@19:02:09.29CEST13-Jul-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @19:02:10.94 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @19:02:11.19 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @19:02:15.39 Polling Can every 10:00 @19:02:17.14 SPE.coast @19:02:17.19 SPE.configure SPEconfig @19:02:17.27 AS.reconfigure ASconfig @19:02:17.31 AS.hold @19:02:17.35 AS.configure ASconfig @19:02:17.44 SS.reconfigure SSconfig @19:02:17.47 SS.hold @19:02:17.52 SS.configure SSconfig @19:02:17.60 CC.reconfigure CCconfig @19:02:17.64 CC.hold @19:02:17.70 CC.configure CCconfig @19:02:17.78 CS.reconfigure CSconfig @19:02:17.82 CS.hold @19:02:17.89 CS.configure CSconfig @19:02:17.97 PC.reconfigure PCconfig @19:02:18.01 PC.hold @19:02:18.42 PC.configure PCconfig @19:02:18.50 PS.configure PSconfig @19:02:18.60 Forearm.reconfigure LooseForearm @19:02:18.64 Forearm.hold @19:02:18.69 Forearm.configure LooseForearm @19:02:18.77 Elbow.reconfigure LooseElbow @19:02:18.80 Elbow.hold @19:02:18.86 Elbow.configure LooseElbow @19:02:18.95 SC.reconfigure SCloose @19:02:18.99 SC.hold @19:02:19.04 SC.configure SCloose @19:02:19.12 SE.reconfigure SEconfig @19:02:19.16 SE.hold @19:02:19.22 SE.configure SEconfig @19:02:19.26 CH.coast @19:02:19.30 CH.configure CHconfig @19:02:19.33 PH.coast @19:02:19.38 PH.configure PHconfig @19:02:19.41 PCR.off @19:02:19.44 PCR.pidtemp= 50,0.1,0,300,25,25 @19:02:19.46 PCR.caltemp= 2.7073,0.0373,0 @19:02:19.49 All dwarf controllers running firmware version 3.75 @19:02:19.60 Cleanly exited at 18:59:23CEST13-Jul-18 @19:02:19.63 Loading /home/esp/esp2/mission/stdMER2017.rb mission @19:02:20.14 AFV.select :carrierSolution @19:02:20.24 delay 2 seconds @19:02:22.27 AS.seek 0.5ml @19:02:51.33 delay 1 second @19:02:52.35 AFV.select :holdingCoil @19:02:52.44 ATV.dial :extSrc @19:02:52.89 AS.reconfigure ASslow1 @19:02:52.93 AS.seek 0.53ml @19:02:59.23 delay 5 seconds @19:03:04.31 AS.reconfigure ASslow2 @19:03:04.35 AS.seek 0.54ml @19:03:14.67 delay 20 seconds @19:03:34.74 AS.reconfigure ASslow1 @19:03:34.78 AS.seek 0.57ml @19:03:41.13 delay 5 seconds @19:03:46.21 AS.reconfigure ASslow2 @19:03:46.25 AS.seek 0.58ml @19:03:56.50 delay 20 seconds @19:04:16.57 AS.reconfigure ASslow1 @19:04:16.61 AS.seek 0.61ml @19:04:22.91 delay 5 seconds @19:04:27.99 AS.reconfigure ASslow2 @19:04:28.03 AS.seek 0.62ml @19:04:38.34 delay 20 seconds @19:04:58.41 AS.reconfigure ASslow1 @19:04:58.45 AS.seek 0.65ml @19:05:04.74 delay 5 seconds @19:05:09.82 AS.reconfigure ASslow2 @19:05:09.86 AS.seek 0.66ml @19:05:20.05 delay 20 seconds @19:05:40.08 AS.reconfigure ASslow1 @19:05:40.12 AS.seek 0.705ml @19:05:49.42 delay 10 seconds @19:05:59.44 ATV.dial :air1 @19:06:01.14 AS.seek 0.725ml @19:06:05.42 delay 3 seconds @19:06:08.44 ATV.dial :waste @19:06:09.08 AS.reconfigure ASslow0 @19:06:09.12 AS.seek :empty @19:07:22.56 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118jul1319h.pcr @19:07:22.58 Building PCR Slug from extSrc with MM2 mix, PP4 primer @19:07:22.59 Priming with carrier @19:07:22.60 ATV.dial ARV @19:07:24.06 ARV.dial :carrier @19:07:24.41 AS.reconfigure ASnorm @19:07:24.45 AS.seek 0.499ml @19:07:50.03 AFV.select :carrierSolution @19:07:50.13 delay 2 seconds @19:07:52.16 AS.reconfigure ASconfig @19:07:52.20 AS.seek 1ml @19:08:21.27 delay 1 second @19:08:22.29 AFV.select :holdingCoil @19:08:22.39 delay 1 second @19:08:23.41 ATV.dial :waste @19:08:25.05 AS.reconfigure ASnorm @19:08:25.10 AS.seek :empty @19:09:15.83 AFV.select :carrierSolution @19:09:15.93 delay 2 seconds @19:09:17.96 AS.reconfigure ASconfig @19:09:18.00 AS.seek 0.5ml @19:09:47.08 delay 1 second @19:09:48.10 AFV.select :holdingCoil @19:09:48.21 Building Slug @19:09:48.22 ATV.dial :ARV @19:09:49.94 delay 2 seconds @19:09:51.96 ARV.dial :air1 @19:09:52.36 delay 2 seconds @19:09:54.43 AS.reconfigure ASslow2 @19:09:54.47 AS.seek 0.506ml @19:10:00.75 delay 2 seconds @19:10:02.77 ARV.dial :MM2 @19:10:04.28 delay 2 seconds @19:10:06.30 Adding MM2 @19:10:06.38 AS.seek 0.524ml @19:10:24.61 delay 25 seconds @19:10:49.63 ARV.dial :PP4 @19:10:50.02 Adding PP4 @19:10:50.03 delay 2 seconds @19:10:52.10 AS.seek 0.53ml @19:10:58.38 delay 10 seconds @19:11:08.41 ARV.dial :air3 @19:11:08.80 adding ARV air @19:11:08.81 delay 2 seconds @19:11:10.88 AS.seek 0.536ml @19:11:17.11 delay 5 seconds @19:11:22.13 ARV.dial :carrier @19:11:23.42 delay 7 seconds @19:11:30.49 AS.seek 0.594ml @19:12:28.99 delay 5 seconds @19:12:34.01 ATV.dial :extSrc @19:12:34.55 delay 2 seconds @19:12:36.62 AS.seek 0.6ml @19:12:42.85 delay 4 seconds @19:12:46.87 ATV.dial ARV @19:12:47.42 delay 2 seconds @19:12:49.44 ARV.dial :carrier @19:12:49.45 delay 2 seconds @19:12:51.52 AS.seek 0.65ml @19:13:42.00 delay 4 seconds @19:13:46.02 ATV.dial PCR @19:13:46.88 ASV.dial :waste1 @19:13:46.96 ARV.dial :air1 @19:13:47.36 Positioning Slug by 0.324ml @19:13:47.44 AS.seek 0.326ml @19:19:13.54 delay 5 seconds @19:19:18.56 ATV.dial ARV @19:19:19.41 ASV.dial :PCRlock @19:19:19.80 delay 5 seconds @19:19:24.82 Running Tt1 Profile60C300 thermocycling @19:19:24.84 PCR.program Profile60C300 @19:19:24.96 Profile60C300 should complete in < 1:45:21 @19:19:24.97 PCR.run 2:16:41.25 @20:40:08.33 PCR.off @20:40:09.83 PCR for Tt1 completed -- closed /var/log/esp/Tt118jul1319h.pcr @20:40:09.89 Cleaning PCR AFV.select :carrierSolution @20:40:09.99 delay 2 seconds @20:40:12.02 AS.reconfigure ASconfig @20:40:12.06 AS.seek 0.5ml @20:40:22.35 delay 1 second @20:40:23.37 AFV.select :holdingCoil @20:40:23.47 ASV.dial :waste1 @20:40:23.87 ATV.dial :air3 @20:40:25.13 AS.reconfigure ASslow1 @20:40:25.17 AS.seek 0.51ml @20:40:27.44 delay 2 seconds @20:40:29.46 ATV.dial :bleach @20:40:30.07 AS.seek 0.81ml @20:41:30.66 delay 3 seconds @20:41:33.68 ATV.dial :air3 @20:41:34.23 delay 3 seconds @20:41:37.30 AS.seek 0.835ml @20:41:42.60 delay 3 seconds @20:41:45.62 ATV.dial :bleach @20:41:46.22 AS.seek 0.82ml @20:41:49.51 delay 3 seconds @20:41:52.53 ARV.dial :waste @20:41:53.15 delay 3 seconds @20:41:56.17 ATV.dial :ARV @20:41:57.14 AS.reconfigure ASconfig @20:41:57.19 AS.seek 0.47ml @20:42:17.59 AFV.select :carrierSolution @20:42:17.69 delay 2 seconds @20:42:19.72 AS.seek 1ml @20:42:50.49 delay 1 second @20:42:51.51 AFV.select :holdingCoil @20:42:51.60 delay 1 second @20:42:52.63 AS.reconfigure ASfast @20:42:52.67 AS.seek 0ml @20:43:20.43 AFV.select :carrierSolution @20:43:20.53 delay 2 seconds @20:43:22.56 AS.reconfigure ASconfig @20:43:22.60 AS.seek 1ml @20:44:20.37 delay 1 second @20:44:21.39 AFV.select :holdingCoil @20:44:21.48 delay 1 second @20:44:22.51 AS.reconfigure ASfast @20:44:22.55 AS.seek 0ml @20:44:50.21 AFV.select :carrierSolution @20:44:50.31 delay 2 seconds @20:44:52.34 AS.reconfigure ASconfig @20:44:52.38 AS.seek 1ml @20:45:50.12 delay 1 second @20:45:51.14 AFV.select :holdingCoil @20:45:51.24 delay 1 second @20:45:52.27 AS.reconfigure ASfast @20:45:52.31 AS.seek 0ml @20:46:19.95 ARV.dial between carrier and air1 @20:46:20.45 ATV.dial :air3 @20:46:21.71 AS.reconfigure ASslow1 @20:46:21.75 AS.seek 0.01ml @20:46:24.49 delay 2 seconds @20:46:26.51 ATV.dial :bleach @20:46:27.12 AS.seek 0.81ml @20:49:08.37 delay 3 seconds @20:49:11.39 ATV.dial :air3 @20:49:11.93 delay 3 seconds @20:49:15.00 AS.seek 0.835ml @20:49:20.33 delay 3 seconds @20:49:23.35 ATV.dial :bleach @20:49:23.96 AS.seek 0.82ml @20:49:27.21 delay 3 seconds @20:49:30.23 ATV.dial PCR @20:49:30.62 AS.reconfigure ASconfig @20:49:30.67 AS.seek :empty @20:50:18.10 PCR.program :bleachProfile @20:50:18.15 PCR program should complete in < 2:45 @20:50:18.16 PCR.run 10:00 @20:53:19.98 PCR.off @20:53:20.01 AFV.select :carrierSolution @20:53:20.11 delay 2 seconds @20:53:22.14 AS.seek 1ml @20:54:19.93 delay 1 second @20:54:20.95 AFV.select :holdingCoil @20:54:21.05 delay 1 second @20:54:22.08 AS.reconfigure ASfast @20:54:22.12 AS.seek 0ml @20:54:49.90 delay 3 seconds @20:54:52.92 AFV.select :carrierSolution @20:54:53.02 delay 2 seconds @20:54:55.05 AS.reconfigure ASconfig @20:54:55.13 AS.seek 1ml @20:55:52.92 delay 1 second @20:55:53.94 AFV.select :holdingCoil @20:55:54.04 delay 1 second @20:55:55.07 AS.reconfigure ASfast @20:55:55.11 AS.seek 0ml @20:56:22.81 delay 3 seconds @20:56:25.83 AFV.select :carrierSolution @20:56:25.93 delay 2 seconds @20:56:27.96 AS.reconfigure ASconfig @20:56:28.01 AS.seek 1ml @20:57:25.76 delay 1 second @20:57:26.78 AFV.select :holdingCoil @20:57:26.88 delay 1 second @20:57:27.91 AS.reconfigure ASfast @20:57:27.95 AS.seek 0ml @20:57:55.67 delay 3 seconds @20:57:58.69 AFV.select :carrierSolution @20:57:58.79 delay 2 seconds @20:58:00.82 AS.reconfigure ASconfig @20:58:00.86 AS.seek 1ml @20:58:58.61 delay 1 second @20:58:59.63 AFV.select :holdingCoil @20:58:59.73 delay 1 second @20:59:00.76 AS.reconfigure ASfast @20:59:00.80 AS.seek 0ml @20:59:28.47 delay 3 seconds @20:59:31.49 AFV.select :carrierSolution @20:59:31.59 delay 2 seconds @20:59:33.62 AS.reconfigure ASconfig @20:59:33.67 AS.seek 1ml @21:00:31.45 delay 1 second @21:00:32.47 AFV.select :holdingCoil @21:00:32.56 delay 1 second @21:00:33.59 AS.reconfigure ASnorm @21:00:33.63 AS.seek 0ml @21:01:24.32
AFV.select :carrierSolution @21:01:24.42 delay 2 seconds @21:01:26.45 AS.reconfigure ASconfig @21:01:26.49 AS.seek 0.5ml @21:01:55.56 delay 1 second @21:01:56.58 AFV.select :holdingCoil @21:01:56.67 ATV.dial :air1 @21:01:58.15 AS.reconfigure ASslow2 @21:01:58.19 AS.seek 0.506ml @21:02:04.61 ATV.dial :extSrc @21:02:06.28 AS.seek 0.512ml @21:02:12.76 delay 5 seconds @21:02:17.78 ATV.dial :air1 @21:02:19.48 AS.reconfigure ASslow1 @21:02:19.52 AS.seek 0.542ml @21:02:25.86 ATV.dial :waste @21:02:26.39 AS.reconfigure ASconfig @21:02:26.43 AS.seek :empty @21:02:58.08 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118jul1321h.pcr @21:02:58.10 Building PCR Slug from extSrc with MM2 mix, PP4 primer @21:02:58.11 Priming with carrier @21:02:58.12 ATV.dial ARV @21:02:59.59 ARV.dial :carrier @21:02:59.93 AS.reconfigure ASnorm @21:02:59.97 AS.seek 0.499ml @21:03:25.53 AFV.select :carrierSolution @21:03:25.63 delay 2 seconds @21:03:27.66 AS.reconfigure ASconfig @21:03:27.70 AS.seek 1ml @21:03:56.76 delay 1 second @21:03:57.78 AFV.select :holdingCoil @21:03:57.88 delay 1 second @21:03:58.90 ATV.dial :waste @21:04:00.54 AS.reconfigure ASnorm @21:04:00.59 AS.seek :empty @21:04:51.33 AFV.select :carrierSolution @21:04:51.43 delay 2 seconds @21:04:53.46 AS.reconfigure ASconfig @21:04:53.50 AS.seek 0.5ml @21:05:22.59 delay 1 second @21:05:23.61 AFV.select :holdingCoil @21:05:23.72 Building Slug @21:05:23.73 ATV.dial :ARV @21:05:25.44 delay 2 seconds @21:05:27.46 ARV.dial :air1 @21:05:27.86 delay 2 seconds @21:05:29.93 AS.reconfigure ASslow2 @21:05:29.98 AS.seek 0.506ml @21:05:36.40 delay 2 seconds @21:05:38.42 ARV.dial :MM2 @21:05:39.92 delay 2 seconds @21:05:41.94 Adding MM2 @21:05:42.00 AS.seek 0.524ml @21:06:00.47 delay 25 seconds @21:06:25.49 ARV.dial :PP4 @21:06:25.87 Adding PP4 @21:06:25.88 delay 2 seconds @21:06:27.95 AS.seek 0.53ml @21:06:34.19 delay 10 seconds @21:06:44.22 ARV.dial :air3 @21:06:44.61 adding ARV air @21:06:44.62 delay 2 seconds @21:06:46.69 AS.seek 0.536ml @21:06:52.94 delay 5 seconds @21:06:57.96 ARV.dial :carrier @21:06:59.25 delay 7 seconds @21:07:06.74 AS.seek 0.594ml @21:08:05.45 delay 5 seconds @21:08:10.47 ATV.dial :extSrc @21:08:11.01 delay 2 seconds @21:08:13.09 AS.seek 0.6ml @21:08:19.33 delay 4 seconds @21:08:23.35 ATV.dial ARV @21:08:23.98 delay 2 seconds @21:08:26.00 ARV.dial :carrier @21:08:26.01 delay 2 seconds @21:08:28.08 AS.seek 0.65ml @21:09:18.62 delay 4 seconds @21:09:22.64 ATV.dial PCR @21:09:23.39 ASV.dial :waste1 @21:09:23.40 ARV.dial :air1 @21:09:23.80 Positioning Slug by 0.324ml @21:09:23.88 AS.seek 0.326ml @21:14:49.92 delay 5 seconds @21:14:54.94 ATV.dial ARV @21:14:55.72 ASV.dial :PCRlock @21:14:56.11 delay 5 seconds @21:15:01.13 Running Tt1 Profile60C300 thermocycling @21:15:01.14 PCR.program Profile60C300 @21:15:01.26 Profile60C300 should complete in < 1:45:21 @21:15:01.27 PCR.run 2:16:41.25 @22:35:42.92 PCR.off @22:35:44.40 PCR for Tt1 completed -- closed /var/log/esp/Tt118jul1321h.pcr @22:35:44.46 Cleaning PCR @22:35:44.47 AFV.select :carrierSolution @22:35:44.57 delay 2 seconds @22:35:46.60 AS.reconfigure ASconfig @22:35:46.64 AS.seek 0.5ml @22:35:56.93 delay 1 second @22:35:57.95 AFV.select :holdingCoil @22:35:58.04 ASV.dial :waste1 @22:35:58.43 ATV.dial :air3 @22:35:59.71 AS.reconfigure ASslow1 @22:35:59.75 AS.seek 0.51ml @22:36:02.02 delay 2 seconds @22:36:04.04 ATV.dial :bleach @22:36:04.64 AS.seek 0.81ml @22:37:05.24 delay 3 seconds @22:37:08.26 ATV.dial :air3 @22:37:08.80 delay 3 seconds @22:37:11.87 AS.seek 0.835ml @22:37:17.08 delay 3 seconds @22:37:20.10 ATV.dial :bleach @22:37:20.71 AS.seek 0.82ml @22:37:23.99 delay 3 seconds @22:37:27.01 ARV.dial :waste @22:37:27.63 delay 3 seconds @22:37:30.65 ATV.dial :ARV @22:37:31.62 AS.reconfigure ASconfig @22:37:31.67 AS.seek 0.47ml @22:37:52.08 AFV.select :carrierSolution @22:37:52.18 delay 2 seconds @22:37:54.21 AS.seek 1ml @22:38:25.35 delay 1 second @22:38:26.37 AFV.select :holdingCoil @22:38:26.47 delay 1 second @22:38:27.50 AS.reconfigure ASfast @22:38:27.54 AS.seek 0ml @22:38:55.32 AFV.select :carrierSolution @22:38:55.42 delay 2 seconds @22:38:57.45 AS.reconfigure ASconfig @22:38:57.49 AS.seek 1ml @22:39:55.22 delay 1 second @22:39:56.24 AFV.select :holdingCoil @22:39:56.34 delay 1 second @22:39:57.37 AS.reconfigure ASfast @22:39:57.41 AS.seek 0ml @22:40:25.07 AFV.select :carrierSolution @22:40:25.17 delay 2 seconds @22:40:27.20 AS.reconfigure ASconfig @22:40:27.24 AS.seek 1ml @22:41:25.02 delay 1 second @22:41:26.04 AFV.select :holdingCoil @22:41:26.14 delay 1 second @22:41:27.17 AS.reconfigure ASfast @22:41:27.21 AS.seek 0ml @22:41:55.01 ARV.dial between carrier and air1 @22:41:55.51 ATV.dial :air3 @22:41:56.77 AS.reconfigure ASslow1 @22:41:56.81 AS.seek 0.01ml @22:41:59.16 delay 2 seconds @22:42:01.18 ATV.dial :bleach @22:42:01.77 AS.seek 0.81ml @22:44:42.91 delay 3 seconds @22:44:45.93 ATV.dial :air3 @22:44:46.46 delay 3 seconds @22:44:49.53 AS.seek 0.835ml @22:44:54.80 delay 3 seconds @22:44:57.82 ATV.dial :bleach @22:44:58.43 AS.seek 0.82ml @22:45:01.71 delay 3 seconds @22:45:04.73 ATV.dial PCR @22:45:05.12 AS.reconfigure ASconfig @22:45:05.16 AS.seek :empty @22:45:52.63 PCR.program :bleachProfile @22:45:52.67 PCR program should complete in < 2:45 @22:45:52.68 PCR.run 10:00 @22:48:54.51 PCR.off @22:48:54.54 AFV.select :carrierSolution @22:48:54.64 delay 2 seconds @22:48:56.67 AS.seek 1ml @22:49:54.49 delay 1 second @22:49:55.51 AFV.select :holdingCoil @22:49:55.61 delay 1 second @22:49:56.64 AS.reconfigure ASfast @22:49:56.68 AS.seek 0ml @22:50:24.27 delay 3 seconds @22:50:27.29 AFV.select :carrierSolution @22:50:27.39 delay 2 seconds @22:50:29.42 AS.reconfigure ASconfig @22:50:29.47 AS.seek 1ml @22:51:27.23 delay 1 second @22:51:28.25 AFV.select :holdingCoil @22:51:28.34 delay 1 second @22:51:29.37 AS.reconfigure ASfast @22:51:29.41 AS.seek 0ml @22:51:57.06 delay 3 seconds @22:52:00.08 AFV.select :carrierSolution @22:52:00.18 delay 2 seconds @22:52:02.21 AS.reconfigure ASconfig @22:52:02.25 AS.seek 1ml @22:52:59.99 delay 1 second @22:53:01.01 AFV.select :holdingCoil @22:53:01.11 delay 1 second @22:53:02.14 AS.reconfigure ASfast @22:53:02.18 AS.seek 0ml @22:53:29.96 delay 3 seconds @22:53:32.98 AFV.select :carrierSolution @22:53:33.08 delay 2 seconds @22:53:35.11 AS.reconfigure ASconfig @22:53:35.16 AS.seek 1ml @22:54:32.93 delay 1 second @22:54:33.95 AFV.select :holdingCoil @22:54:34.05 delay 1 second @22:54:35.08 AS.reconfigure ASfast @22:54:35.12 AS.seek 0ml @22:55:02.98 delay 3 seconds @22:55:06.00 AFV.select :carrierSolution @22:55:06.10 delay 2 seconds @22:55:08.13 AS.reconfigure ASconfig @22:55:08.18 AS.seek 1ml @22:56:05.92 delay 1 second @22:56:06.94 AFV.select :holdingCoil @22:56:07.03 delay 1 second @22:56:08.06 AS.reconfigure ASnorm @22:56:08.10 AS.seek 0ml @22:56:58.82
AFV.select :carrierSolution @22:56:58.92 delay 2 seconds @22:57:00.95 AS.reconfigure ASconfig @22:57:00.99 AS.seek 0.5ml @22:57:30.04 delay 1 second @22:57:31.06 AFV.select :holdingCoil @22:57:31.16 ATV.dial :air1 @22:57:32.64 AS.reconfigure ASslow2 @22:57:32.68 AS.seek 0.51ml @22:57:43.01 delay 5 seconds @22:57:48.03 ATV.dial :extSrc @22:57:49.71 AS.seek 0.53ml @22:58:09.98 delay 10 seconds @22:58:20.00 ATV.dial :air1 @22:58:21.67 AS.reconfigure ASslow1 @22:58:21.71 AS.seek 0.56ml @22:58:28.01 ATV.dial :waste @22:58:28.54 AS.reconfigure ASconfig @22:58:28.58 AS.seek :empty @22:59:01.27 Setting up PCR for Tt2 -- writing /var/log/esp/Tt218jul1322h.pcr @22:59:01.28 Building PCR Slug from extSrc with MM3 mix, PP6 primer @22:59:01.30 Priming with carrier ATV.dial ARV @22:59:02.78 ARV.dial :carrier @22:59:03.12 AS.reconfigure ASnorm @22:59:03.16 AS.seek 0.499ml @22:59:28.74 AFV.select :carrierSolution @22:59:28.84 delay 2 seconds @22:59:30.87 AS.reconfigure ASconfig @22:59:30.91 AS.seek 1ml @22:59:59.98 delay 1 second @23:00:01.00 AFV.select :holdingCoil @23:00:01.10 delay 1 second @23:00:02.12 ATV.dial :waste @23:00:03.76 AS.reconfigure ASnorm @23:00:03.81 AS.seek :empty @23:00:54.86 AFV.select :carrierSolution @23:00:55.03 delay 2 seconds @23:00:57.06 AS.reconfigure ASconfig @23:00:57.10 AS.seek 0.5ml @23:01:26.20 delay 1 second @23:01:27.22 AFV.select :holdingCoil @23:01:27.32 Building Slug @23:01:27.33 ATV.dial :ARV @23:01:29.06 delay 2 seconds @23:01:31.08 ARV.dial :air1 @23:01:31.47 delay 2 seconds @23:01:33.54 AS.reconfigure ASslow2 @23:01:33.59 AS.seek 0.506ml @23:01:39.87 delay 2 seconds @23:01:41.89 ARV.dial :MM3 @23:01:42.95 delay 2 seconds @23:01:44.97 Adding MM3 @23:01:45.05 AS.seek 0.524ml @23:02:03.51 delay 25 seconds @23:02:28.53 ARV.dial :PP6 @23:02:29.01 Adding PP6 @23:02:29.02 delay 2 seconds @23:02:31.09 AS.seek 0.53ml @23:02:37.51 delay 10 seconds @23:02:47.54 ARV.dial :air1 @23:02:48.39 adding ARV air @23:02:48.40 delay 2 seconds @23:02:50.47 AS.seek 0.536ml @23:02:56.67 delay 5 seconds @23:03:01.69 ARV.dial :carrier @23:03:02.09 delay 7 seconds @23:03:09.17 AS.seek 0.594ml @23:04:07.73 delay 5 seconds @23:04:12.75 ATV.dial :extSrc @23:04:13.29 delay 2 seconds @23:04:15.36 AS.seek 0.6ml @23:04:21.59 delay 4 seconds @23:04:25.61 ATV.dial ARV @23:04:26.15 delay 2 seconds @23:04:28.17 ARV.dial :carrier @23:04:28.18 delay 2 seconds @23:04:30.25 AS.seek 0.65ml @23:05:20.75 delay 4 seconds @23:05:24.77 ATV.dial PCR @23:05:25.53 ASV.dial :waste1 @23:05:25.54 ARV.dial :air1 @23:05:25.94 Positioning Slug by 0.324ml @23:05:26.01 AS.seek 0.326ml @23:10:52.09 delay 5 seconds @23:10:57.11 ATV.dial ARV @23:10:57.87 ASV.dial :PCRlock @23:10:58.26 delay 5 seconds @23:11:03.28 Running Tt2 Profile60C300 thermocycling @23:11:03.30 PCR.program Profile60C300 @23:11:03.42 Profile60C300 should complete in < 1:45:21 @23:11:03.43 PCR.run 2:16:41.25 @00:31:55.18CEST14-Jul-18 PCR.off @00:31:56.69 PCR for Tt2 completed -- closed /var/log/esp/Tt218jul1322h.pcr @00:31:56.75 Cleaning PCR @00:31:56.76 AFV.select :carrierSolution @00:31:56.86 delay 2 seconds @00:31:58.89 AS.reconfigure ASconfig @00:31:58.93 AS.seek 0.5ml @00:32:09.25 delay 1 second @00:32:10.27 AFV.select :holdingCoil @00:32:10.36 ASV.dial :waste1 @00:32:10.75 ATV.dial :air3 @00:32:12.03 AS.reconfigure ASslow1 @00:32:12.07 AS.seek 0.51ml @00:32:14.39 delay 2 seconds @00:32:16.40 ATV.dial :bleach @00:32:17.01 AS.seek 0.81ml @00:33:17.58 delay 3 seconds @00:33:20.60 ATV.dial :air3 @00:33:21.14 delay 3 seconds @00:33:24.21 AS.seek 0.835ml @00:33:29.53 delay 3 seconds @00:33:32.55 ATV.dial :bleach @00:33:33.16 AS.seek 0.82ml @00:33:36.41 delay 3 seconds @00:33:39.43 ARV.dial :waste @00:33:40.05 delay 3 seconds @00:33:43.07 ATV.dial :ARV @00:33:44.05 AS.reconfigure ASconfig @00:33:44.09 AS.seek 0.47ml @00:34:04.49 AFV.select :carrierSolution @00:34:04.59 delay 2 seconds @00:34:06.62 AS.seek 1ml @00:34:37.36 delay 1 second @00:34:38.38 AFV.select :holdingCoil @00:34:38.48 delay 1 second @00:34:39.51 AS.reconfigure ASfast @00:34:39.55 AS.seek 0ml @00:35:07.28 AFV.select :carrierSolution @00:35:07.38 delay 2 seconds @00:35:09.41 AS.reconfigure ASconfig @00:35:09.45 AS.seek 1ml @00:36:07.22 delay 1 second @00:36:08.24 AFV.select :holdingCoil @00:36:08.34 delay 1 second @00:36:09.37 AS.reconfigure ASfast @00:36:09.41 AS.seek 0ml @00:36:37.36 AFV.select :carrierSolution @00:36:37.46 delay 2 seconds @00:36:39.49 AS.reconfigure ASconfig @00:36:39.53 AS.seek 1ml @00:37:37.28 delay 1 second @00:37:38.30 AFV.select :holdingCoil @00:37:38.40 delay 1 second @00:37:39.43 AS.reconfigure ASfast @00:37:39.47 AS.seek 0ml @00:38:07.14 ARV.dial between carrier and air1 @00:38:07.64 ATV.dial :air3 @00:38:08.91 AS.reconfigure ASslow1 @00:38:08.95 AS.seek 0.01ml @00:38:11.27 delay 2 seconds @00:38:13.29 ATV.dial :bleach @00:38:13.88 AS.seek 0.81ml @00:40:55.05 delay 3 seconds @00:40:58.07 ATV.dial :air3 @00:40:58.61 delay 3 seconds @00:41:01.68 AS.seek 0.835ml @00:41:06.96 delay 3 seconds @00:41:09.98 ATV.dial :bleach @00:41:10.58 AS.seek 0.82ml @00:41:13.86 delay 3 seconds @00:41:16.88 ATV.dial PCR @00:41:17.27 AS.reconfigure ASconfig @00:41:17.31 AS.seek :empty @00:42:04.77 PCR.program :bleachProfile @00:42:04.82 PCR program should complete in < 2:45 @00:42:04.83 PCR.run 10:00 @00:45:06.66 PCR.off @00:45:06.69 AFV.select :carrierSolution @00:45:06.78 delay 2 seconds @00:45:08.81 AS.seek 1ml @00:46:06.63 delay 1 second @00:46:07.65 AFV.select :holdingCoil @00:46:07.75 delay 1 second @00:46:08.78 AS.reconfigure ASfast @00:46:08.82 AS.seek 0ml @00:46:36.97 delay 3 seconds @00:46:39.99 AFV.select :carrierSolution @00:46:40.09 delay 2 seconds @00:46:42.12 AS.reconfigure ASconfig @00:46:42.16 AS.seek 1ml @00:47:39.91 delay 1 second @00:47:40.93 AFV.select :holdingCoil @00:47:41.03 delay 1 second @00:47:42.06 AS.reconfigure ASfast @00:47:42.10 AS.seek 0ml @00:48:09.77 delay 3 seconds @00:48:12.79 AFV.select :carrierSolution @00:48:12.89 delay 2 seconds @00:48:14.92 AS.reconfigure ASconfig @00:48:14.97 AS.seek 1ml @00:49:12.72 delay 1 second @00:49:13.74 AFV.select :holdingCoil @00:49:13.84 delay 1 second @00:49:14.87 AS.reconfigure ASfast @00:49:14.91 AS.seek 0ml @00:49:42.54 delay 3 seconds @00:49:45.56 AFV.select :carrierSolution @00:49:45.66 delay 2 seconds @00:49:47.69 AS.reconfigure ASconfig @00:49:47.73 AS.seek 1ml @00:50:45.47 delay 1 second @00:50:46.49 AFV.select :holdingCoil @00:50:46.59 delay 1 second @00:50:47.62 AS.reconfigure ASfast @00:50:47.66 AS.seek 0ml @00:51:15.27 delay 3 seconds @00:51:18.29 AFV.select :carrierSolution @00:51:18.39 delay 2 seconds @00:51:20.42 AS.reconfigure ASconfig @00:51:20.47 AS.seek 1ml @00:52:18.26 delay 1 second @00:52:19.28 AFV.select :holdingCoil @00:52:19.38 delay 1 second @00:52:20.41 AS.reconfigure ASnorm @00:52:20.45 AS.seek 0ml @00:53:11.16
AFV.select :carrierSolution @00:53:11.26 delay 2 seconds @00:53:13.29 AS.reconfigure ASconfig @00:53:13.33 AS.seek 0.5ml @00:53:42.38 delay 1 second @00:53:43.40 AFV.select :holdingCoil @00:53:43.49 ATV.dial :air1 @00:53:44.97 AS.reconfigure ASslow2 @00:53:45.01 AS.seek 0.506ml @00:53:51.49 ATV.dial :extSrc @00:53:53.16 AS.seek 0.512ml @00:53:59.43 delay 5 seconds @00:54:04.45 ATV.dial :air1 @00:54:06.14 AS.reconfigure ASslow1 @00:54:06.18 AS.seek 0.542ml @00:54:12.49 ATV.dial :waste @00:54:13.02 AS.reconfigure ASconfig @00:54:13.06 AS.seek :empty @00:54:44.69 Setting up PCR for Tt2 -- writing /var/log/esp/Tt218jul1400h.pcr @00:54:44.71 Building PCR Slug from extSrc with MM3 mix, PP6 primer @00:54:44.72 Priming with carrier @00:54:44.73 ATV.dial ARV @00:54:46.29 ARV.dial :carrier @00:54:46.63 AS.reconfigure ASnorm @00:54:46.67 AS.seek 0.499ml @00:55:12.26 AFV.select :carrierSolution @00:55:12.36 delay 2 seconds @00:55:14.39 AS.reconfigure ASconfig @00:55:14.43 AS.seek 1ml @00:55:43.49 delay 1 second @00:55:44.51 AFV.select :holdingCoil @00:55:44.61 delay 1 second @00:55:45.63 ATV.dial :waste @00:55:47.27 AS.reconfigure ASnorm @00:55:47.32 AS.seek :empty @00:56:38.05 AFV.select :carrierSolution @00:56:38.15 delay 2 seconds @00:56:40.18 AS.reconfigure ASconfig @00:56:40.22 AS.seek 0.5ml @00:57:09.34 delay 1 second @00:57:10.36 AFV.select :holdingCoil @00:57:10.46 Building Slug @00:57:10.47 ATV.dial :ARV @00:57:12.19 delay 2 seconds @00:57:14.21 ARV.dial :air1 @00:57:14.61 delay 2 seconds @00:57:16.68 AS.reconfigure ASslow2 @00:57:16.73 AS.seek 0.506ml @00:57:22.98 delay 2 seconds @00:57:25.00 ARV.dial :MM3 @00:57:26.07 delay 2 seconds @00:57:28.09 Adding MM3 @00:57:28.16 AS.seek 0.524ml @00:57:46.41 delay 25 seconds @00:58:11.43 ARV.dial :PP6 @00:58:11.82 Adding PP6 @00:58:11.83 delay 2 seconds @00:58:13.90 AS.seek 0.53ml @00:58:20.18 delay 10 seconds @00:58:30.21 ARV.dial :air1 @00:58:31.04 adding ARV air @00:58:31.05 delay 2 seconds @00:58:33.12 AS.seek 0.536ml @00:58:39.51 delay 5 seconds @00:58:44.53 ARV.dial :carrier @00:58:44.93 delay 7 seconds @00:58:52.01 AS.seek 0.594ml @00:59:50.60 delay 5 seconds @00:59:55.62 ATV.dial :extSrc @00:59:56.54 delay 2 seconds @00:59:58.61 AS.seek 0.6ml @01:00:04.79 delay 4 seconds @01:00:08.81 ATV.dial ARV @01:00:09.45 delay 2 seconds @01:00:11.47 ARV.dial :carrier @01:00:11.48 delay 2 seconds @01:00:13.55 AS.seek 0.65ml @01:01:04.04 delay 4 seconds @01:01:08.06 ATV.dial PCR @01:01:08.80 ASV.dial :waste1 @01:01:08.81 ARV.dial :air1 @01:01:09.28 Positioning Slug by 0.324ml @01:01:09.36 AS.seek 0.326ml @01:06:35.65 delay 5 seconds @01:06:40.67 ATV.dial ARV @01:06:41.53 ASV.dial :PCRlock @01:06:41.92 delay 5 seconds @01:06:46.94 Running Tt2 Profile60C300 thermocycling @01:06:46.96 PCR.program Profile60C300 @01:06:47.08 Profile60C300 should complete in < 1:45:21 @01:06:47.09 PCR.run 2:16:41.25 @02:27:32.26 PCR.off @02:27:33.74 PCR for Tt2 completed -- closed /var/log/esp/Tt218jul1400h.pcr @02:27:33.80 Cleaning PCR @02:27:33.81 AFV.select :carrierSolution @02:27:33.91 delay 2 seconds @02:27:35.94 AS.reconfigure ASconfig @02:27:35.98 AS.seek 0.5ml @02:27:46.28 delay 1 second @02:27:47.30 AFV.select :holdingCoil @02:27:47.39 ASV.dial :waste1 @02:27:47.78 ATV.dial :air3 @02:27:49.04 AS.reconfigure ASslow1 @02:27:49.08 AS.seek 0.51ml @02:27:51.38 delay 2 seconds @02:27:53.39 ATV.dial :bleach @02:27:54.00 AS.seek 0.81ml @02:28:54.59 delay 3 seconds @02:28:57.61 ATV.dial :air3 @02:28:58.15 delay 3 seconds @02:29:01.22 AS.seek 0.835ml @02:29:06.43 delay 3 seconds @02:29:09.45 ATV.dial :bleach @02:29:10.45 AS.seek 0.82ml @02:29:13.72 delay 3 seconds @02:29:16.74 ARV.dial :waste @02:29:17.36 delay 3 seconds @02:29:20.38 ATV.dial :ARV @02:29:21.35 AS.reconfigure ASconfig @02:29:21.39 AS.seek 0.47ml @02:29:41.80 AFV.select :carrierSolution @02:29:41.90 delay 2 seconds @02:29:43.93 AS.seek 1ml @02:30:14.67 delay 1 second @02:30:15.69 AFV.select :holdingCoil @02:30:15.79 delay 1 second @02:30:16.82 AS.reconfigure ASfast @02:30:16.86 AS.seek 0ml @02:30:44.51 AFV.select :carrierSolution @02:30:44.61 delay 2 seconds @02:30:46.64 AS.reconfigure ASconfig @02:30:46.68 AS.seek 1ml @02:31:44.44 delay 1 second @02:31:45.46 AFV.select :holdingCoil @02:31:45.56 delay 1 second @02:31:46.59 AS.reconfigure ASfast @02:31:46.63 AS.seek 0ml @02:32:14.33 AFV.select :carrierSolution @02:32:14.43 delay 2 seconds @02:32:16.46 AS.reconfigure ASconfig @02:32:16.50 AS.seek 1ml @02:33:14.28 delay 1 second @02:33:15.30 AFV.select :holdingCoil @02:33:15.40 delay 1 second @02:33:16.43 AS.reconfigure ASfast @02:33:16.47 AS.seek 0ml @02:33:44.22 ARV.dial between carrier and air1 @02:33:44.72 ATV.dial :air3 @02:33:45.98 AS.reconfigure ASslow1 @02:33:46.02 AS.seek 0.01ml @02:33:48.33 delay 2 seconds @02:33:50.35 ATV.dial :bleach @02:33:50.93 AS.seek 0.81ml @02:36:32.19 delay 3 seconds @02:36:35.21 ATV.dial :air3 @02:36:35.73 delay 3 seconds @02:36:38.80 AS.seek 0.835ml @02:36:44.13 delay 3 seconds @02:36:47.15 ATV.dial :bleach @02:36:47.76 AS.seek 0.82ml @02:36:51.02 delay 3 seconds @02:36:54.04 ATV.dial PCR @02:36:54.44 AS.reconfigure ASconfig @02:36:54.48 AS.seek :empty @02:37:41.92 PCR.program :bleachProfile @02:37:41.96 PCR program should complete in < 2:45 @02:37:41.97 PCR.run 10:00 @02:40:43.80 PCR.off @02:40:43.82 AFV.select :carrierSolution @02:40:43.92 delay 2 seconds @02:40:45.95 AS.seek 1ml @02:41:43.78 delay 1 second @02:41:44.80 AFV.select :holdingCoil @02:41:44.90 delay 1 second @02:41:45.93 AS.reconfigure ASfast @02:41:45.97 AS.seek 0ml @02:42:13.69 delay 3 seconds @02:42:16.71 AFV.select :carrierSolution @02:42:16.81 delay 2 seconds @02:42:18.84 AS.reconfigure ASconfig @02:42:18.89 AS.seek 1ml @02:43:16.64 delay 1 second @02:43:17.66 AFV.select :holdingCoil @02:43:17.76 delay 1 second @02:43:18.79 AS.reconfigure ASfast @02:43:18.83 AS.seek 0ml @02:43:46.49 delay 3 seconds @02:43:49.51 AFV.select :carrierSolution @02:43:49.61 delay 2 seconds @02:43:51.64 AS.reconfigure ASconfig @02:43:51.68 AS.seek 1ml @02:44:49.44 delay 1 second @02:44:50.46 AFV.select :holdingCoil @02:44:50.56 delay 1 second @02:44:51.59 AS.reconfigure ASfast @02:44:51.63 AS.seek 0ml @02:45:19.30 delay 3 seconds @02:45:22.32 AFV.select :carrierSolution @02:45:22.42 delay 2 seconds @02:45:24.45 AS.reconfigure ASconfig @02:45:24.50 AS.seek 1ml @02:46:22.28 delay 1 second @02:46:23.30 AFV.select :holdingCoil @02:46:23.40 delay 1 second @02:46:24.43 AS.reconfigure ASfast @02:46:24.47 AS.seek 0ml @02:46:52.35 delay 3 seconds @02:46:55.37 AFV.select :carrierSolution @02:46:55.47 delay 2 seconds @02:46:57.50 AS.reconfigure ASconfig @02:46:57.55 AS.seek 1ml @02:47:55.28 delay 1 second @02:47:56.30 AFV.select :holdingCoil @02:47:56.39 delay 1 second @02:47:57.42 AS.reconfigure ASnorm @02:47:57.46 AS.seek 0ml @02:48:48.20
AFV.select :carrierSolution @02:48:48.30 delay 2 seconds @02:48:50.33 AS.reconfigure ASconfig @02:48:50.75 AS.seek 0.5ml @02:49:19.78 delay 1 second @02:49:20.80 AFV.select :holdingCoil @02:49:20.90 ATV.dial :air1 @02:49:22.39 AS.reconfigure ASslow1 @02:49:22.43 AS.seek 0.52ml @02:49:26.77 delay 5 seconds @02:49:31.79 ATV.dial :extSrc @02:49:33.47 AS.reconfigure ASslow2 @02:49:33.51 AS.seek 0.545ml @02:49:58.99 delay 10 seconds @02:50:09.01 ATV.dial :air1 @02:50:10.70 AS.reconfigure ASslow1 @02:50:10.74 AS.seek 0.575ml @02:50:17.00 ATV.dial :waste @02:50:17.53 AS.reconfigure ASconfig @02:50:17.57 AS.seek :empty @02:50:50.97 AFV.select :carrierSolution @02:50:51.06 delay 2 seconds @02:50:53.09 AS.seek 0.5ml @02:51:22.14 delay 1 second @02:51:23.16 AFV.select :holdingCoil @02:51:23.26 ATV.dial :air1 @02:51:23.86 AS.reconfigure ASslow1 @02:51:23.90 AS.seek 0.52ml @02:51:28.21 delay 5 seconds @02:51:33.23 ATV.dial :extSrc @02:51:34.90 AS.reconfigure ASslow2 @02:51:34.95 AS.seek 0.538ml @02:51:53.24 delay 10 seconds @02:52:03.26 ATV.dial :air1 @02:52:04.95 AS.reconfigure ASslow1 @02:52:04.99 AS.seek 0.568ml @02:52:11.36 ATV.dial :waste @02:52:12.00 AS.reconfigure ASconfig @02:52:12.04 AS.seek :empty @02:52:45.02 deconEluateRecoveryLine extSrc @02:52:45.03 ATV.dial :waste @02:52:45.04 AS.reconfigure ASnorm @02:52:45.08 AS.seek 0ml @02:52:45.39 ATV.dial :air3 @02:52:46.06 AS.reconfigure ASslow1 @02:52:46.10 AS.seek 0.01ml @02:52:48.41 delay 2 seconds @02:52:50.43 ATV.dial :bleach @02:52:50.89 AS.seek 0.31ml @02:53:51.52 delay 3 seconds @02:53:54.54 ATV.dial :air3 @02:53:55.18 delay 3 seconds @02:53:58.25 AS.seek 0.335ml @02:54:03.48 delay 3 seconds @02:54:06.50 ATV.dial :bleach @02:54:07.10 AS.seek 0.32ml @02:54:10.39 delay 3 seconds @02:54:13.41 ATV.dial :extSrc @02:54:14.03 AS.seek 0ml @02:55:18.63 AFV.select :carrierSolution @02:55:18.73 delay 2 seconds @02:55:20.76 AS.reconfigure ASconfig @02:55:20.80 AS.seek 0.5ml @02:55:49.83 delay 1 second @02:55:50.85 AFV.select :holdingCoil @02:55:50.95 ATV.dial :extSrc @02:55:50.97 AS.reconfigure ASslow1 @02:55:51.01 AS.seek 0ml @02:57:31.86 AFV.select :carrierSolution @02:57:31.96 delay 2 seconds @02:57:33.99 AS.reconfigure ASconfig @02:57:34.03 AS.seek 0.5ml @02:58:03.05 delay 1 second @02:58:04.07 AFV.select :holdingCoil @02:58:04.17 ATV.dial :extSrc @02:58:04.19 AS.reconfigure ASslow1 @02:58:04.23 AS.seek 0ml @02:59:45.04 AFV.select :carrierSolution @02:59:45.14 delay 2 seconds @02:59:47.17 AS.reconfigure ASconfig @02:59:47.21 AS.seek 0.5ml @03:00:16.29 delay 1 second @03:00:17.31 AFV.select :holdingCoil @03:00:17.41 ATV.dial :extSrc @03:00:17.43 AS.reconfigure ASslow1 @03:00:17.47 AS.seek 0ml @03:01:58.29 AFV.select :carrierSolution @03:01:58.39 delay 2 seconds @03:02:00.42 AS.reconfigure ASconfig @03:02:00.46 AS.seek 0.5ml @03:02:29.49 delay 1 second @03:02:30.51 AFV.select :holdingCoil @03:02:30.61 ATV.dial :extSrc @03:02:30.63 AS.reconfigure ASslow1 @03:02:30.67 AS.seek 0ml @03:04:11.49 ATV.dial :air2 @03:04:12.80 AS.reconfigure ASslow0 @03:04:12.84 AS.seek 0.5ml @03:05:03.40 ATV.dial :extSrc @03:05:04.86 AS.seek 0.02ml @03:05:53.40 delay 1 second @03:05:54.42 ATV.dial :waste @03:05:55.69 AS.reconfigure ASconfig @03:05:55.73 AS.seek :empty @03:05:57.23 deconEluateRecoveryLine extSrc @03:05:57.24 ATV.dial :waste @03:05:57.25 AS.reconfigure ASnorm @03:05:57.29 AS.seek 0ml @03:05:57.63 ATV.dial :air3 @03:05:58.45 AS.reconfigure ASslow1 @03:05:58.49 AS.seek 0.01ml @03:06:00.86 delay 2 seconds @03:06:02.88 ATV.dial :bleach @03:06:03.33 AS.seek 0.31ml @03:07:04.02 delay 3 seconds @03:07:07.04 ATV.dial :air3 @03:07:07.58 delay 3 seconds @03:07:10.65 AS.seek 0.335ml @03:07:15.98 delay 3 seconds @03:07:19.00 ATV.dial :bleach @03:07:19.61 AS.seek 0.32ml @03:07:22.83 delay 3 seconds @03:07:25.85 ATV.dial :extSrc @03:07:26.47 AS.seek 0ml @03:08:31.09 AFV.select :carrierSolution @03:08:31.19 delay 2 seconds @03:08:33.22 AS.reconfigure ASconfig @03:08:33.26 AS.seek 0.5ml @03:09:02.29 delay 1 second @03:09:03.31 AFV.select :holdingCoil @03:09:03.41 ATV.dial :extSrc @03:09:03.43 AS.reconfigure ASslow1 @03:09:03.47 AS.seek 0ml @03:10:44.29 AFV.select :carrierSolution @03:10:44.39 delay 2 seconds @03:10:46.42 AS.reconfigure ASconfig @03:10:46.46 AS.seek 0.5ml @03:11:15.49 delay 1 second @03:11:16.51 AFV.select :holdingCoil @03:11:16.61 ATV.dial :extSrc @03:11:16.63 AS.reconfigure ASslow1 @03:11:16.67 AS.seek 0ml @03:12:57.51 AFV.select :carrierSolution @03:12:57.61 delay 2 seconds @03:12:59.64 AS.reconfigure ASconfig @03:12:59.68 AS.seek 0.5ml @03:13:28.71 delay 1 second @03:13:29.73 AFV.select :holdingCoil @03:13:29.83 ATV.dial :extSrc @03:13:29.85 AS.reconfigure ASslow1 @03:13:29.89 AS.seek 0ml @03:15:10.73 AFV.select :carrierSolution @03:15:10.83 delay 2 seconds @03:15:12.86 AS.reconfigure ASconfig @03:15:12.90 AS.seek 0.5ml @03:15:41.93 delay 1 second @03:15:42.95 AFV.select :holdingCoil @03:15:43.05 ATV.dial :extSrc @03:15:43.07 AS.reconfigure ASslow1 @03:15:43.11 AS.seek 0ml @03:17:23.93 ATV.dial :air2 @03:17:25.24 AS.reconfigure ASslow0 @03:17:25.28 AS.seek 0.5ml @03:18:15.88 ATV.dial :extSrc @03:18:17.35 AS.seek 0.02ml @03:19:05.92 delay 1 second @03:19:06.94 ATV.dial :waste @03:19:08.21 AS.reconfigure ASconfig @03:19:08.25 AS.seek :empty @03:19:09.71 deconEluateRecoveryLine extSrc @03:19:09.72 ATV.dial :waste @03:19:09.73 AS.reconfigure ASnorm @03:19:09.77 AS.seek 0ml @03:19:10.10 ATV.dial :air3 @03:19:10.91 AS.reconfigure ASslow1 @03:19:10.96 AS.seek 0.01ml @03:19:13.24 delay 2 seconds @03:19:15.26 ATV.dial :bleach @03:19:15.71 AS.seek 0.31ml @03:20:16.31 delay 3 seconds @03:20:19.33 ATV.dial :air3 @03:20:19.87 delay 3 seconds @03:20:22.94 AS.seek 0.335ml @03:20:28.24 delay 3 seconds @03:20:31.26 ATV.dial :bleach @03:20:31.87 AS.seek 0.32ml @03:20:35.20 delay 3 seconds @03:20:38.22 ATV.dial :extSrc @03:20:38.93 AS.seek 0ml @03:21:43.54 AFV.select :carrierSolution @03:21:43.64 delay 2 seconds @03:21:45.67 AS.reconfigure ASconfig @03:21:45.71 AS.seek 0.5ml @03:22:14.75 delay 1 second @03:22:15.77 AFV.select :holdingCoil @03:22:15.87 ATV.dial :extSrc @03:22:15.89 AS.reconfigure ASslow1 @03:22:15.93 AS.seek 0ml @03:23:56.75 AFV.select :carrierSolution @03:23:56.85 delay 2 seconds @03:23:58.88 AS.reconfigure ASconfig @03:23:58.92 AS.seek 0.5ml @03:24:27.93 delay 1 second @03:24:28.95 AFV.select :holdingCoil @03:24:29.05 ATV.dial :extSrc @03:24:29.07 AS.reconfigure ASslow1 @03:24:29.11 AS.seek 0ml @03:26:09.95 AFV.select :carrierSolution @03:26:10.05 delay 2 seconds @03:26:12.08 AS.reconfigure ASconfig @03:26:12.12 AS.seek 0.5ml @03:26:41.15 delay 1 second @03:26:42.17 AFV.select :holdingCoil @03:26:42.27 ATV.dial :extSrc @03:26:42.29 AS.reconfigure ASslow1 @03:26:42.33 AS.seek 0ml @03:28:23.12 AFV.select :carrierSolution @03:28:23.22 delay 2 seconds @03:28:25.25 AS.reconfigure ASconfig @03:28:25.29 AS.seek 0.5ml @03:28:54.36 delay 1 second @03:28:55.38 AFV.select :holdingCoil @03:28:55.48 ATV.dial :extSrc @03:28:55.50 AS.reconfigure ASslow1 @03:28:55.54 AS.seek 0ml @03:30:36.37 ATV.dial :air2 @03:30:37.99 AS.reconfigure ASslow0 @03:30:38.10 AS.seek 0.5ml @03:31:28.70 ATV.dial :extSrc @03:31:30.17 AS.seek 0.02ml @03:32:18.75 delay 1 second @03:32:19.77 ATV.dial :waste @03:32:21.04 AS.reconfigure ASconfig @03:32:21.08 AS.seek :empty @15:43:27.25CEST14-Jul-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @15:43:28.89 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @15:43:29.13 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @15:43:33.37 Polling Can every 10:00 @15:43:35.12 SPE.coast @15:43:35.16 SPE.configure SPEconfig @15:43:35.24 AS.reconfigure ASconfig @15:43:35.28 AS.hold @15:43:35.35 AS.configure ASconfig @15:43:35.43 SS.reconfigure SSconfig @15:43:35.47 SS.hold @15:43:35.53 SS.configure SSconfig @15:43:35.61 CC.reconfigure CCconfig @15:43:35.65 CC.hold @15:43:35.72 CC.configure CCconfig @15:43:35.80 CS.reconfigure CSconfig @15:43:35.83 CS.hold @15:43:35.91 CS.configure CSconfig @15:43:35.99 PC.reconfigure PCconfig @15:43:36.33 PC.hold @15:43:36.43 PC.configure PCconfig @15:43:36.51 PS.configure PSconfig @15:43:36.60 Forearm.reconfigure LooseForearm @15:43:36.64 Forearm.hold @15:43:36.69 Forearm.configure LooseForearm @15:43:36.77 Elbow.reconfigure LooseElbow @15:43:36.81 Elbow.hold @15:43:36.87 Elbow.configure LooseElbow @15:43:36.96 SC.reconfigure SCloose @15:43:37.00 SC.hold @15:43:37.07 SC.configure SCloose @15:43:37.15 SE.reconfigure SEconfig @15:43:37.19 SE.hold @15:43:37.23 SE.configure SEconfig @15:43:37.27 CH.coast @15:43:37.32 CH.configure CHconfig @15:43:37.35 PH.coast @15:43:37.40 PH.configure PHconfig @15:43:37.43 PCR.off @15:43:37.46 PCR.pidtemp= 50,0.1,0,300,25,25 @15:43:37.49 PCR.caltemp= 2.7073,0.0373,0 @15:43:37.52 All dwarf controllers running firmware version 3.75 @15:43:37.63 Cleanly exited at 15:42:40CEST14-Jul-18 @15:43:37.66 Loading /home/esp/esp2/mission/stdMER2017.rb mission @15:43:38.19 AFV.select :carrierSolution @15:43:38.29 delay 2 seconds @15:43:40.32 AS.seek 0.5ml @15:44:09.36 delay 1 second @15:44:10.38 AFV.select :holdingCoil @15:44:10.48 ATV.dial :extSrc @15:44:11.91 AS.reconfigure ASslow1 @15:44:11.95 AS.seek 0.53ml @15:44:18.28 delay 5 seconds @15:44:23.36 AS.reconfigure ASslow2 @15:44:23.40 AS.seek 0.54ml @15:44:33.67 delay 20 seconds @15:44:53.74 AS.reconfigure ASslow1 @15:44:53.78 AS.seek 0.57ml @15:45:00.08 delay 5 seconds @15:45:05.16 AS.reconfigure ASslow2 @15:45:05.21 AS.seek 0.58ml @15:45:15.47 delay 20 seconds @15:45:35.54 AS.reconfigure ASslow1 @15:45:35.58 AS.seek 0.61ml @15:45:41.86 delay 5 seconds @15:45:46.94 AS.reconfigure ASslow2 @15:45:46.98 AS.seek 0.62ml @15:45:58.00 delay 20 seconds @15:46:18.07 AS.reconfigure ASslow1 @15:46:18.11 AS.seek 0.65ml @15:46:24.41 delay 5 seconds @15:46:29.49 AS.reconfigure ASslow2 @15:46:29.53 AS.seek 0.66ml @16:09:26.15CEST14-Jul-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @16:09:27.81 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @16:09:28.05 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @16:09:32.31 Polling Can every 10:00 @16:09:34.05 SPE.coast @16:09:34.09 SPE.configure SPEconfig @16:09:34.17 AS.reconfigure ASconfig @16:09:34.21 AS.hold @16:09:34.26 AS.configure ASconfig @16:09:34.34 SS.reconfigure SSconfig @16:09:34.38 SS.hold @16:09:34.43 SS.configure SSconfig @16:09:34.51 CC.reconfigure CCconfig @16:09:34.55 CC.hold @16:09:34.60 CC.configure CCconfig @16:09:34.68 CS.reconfigure CSconfig @16:09:34.72 CS.hold @16:09:34.79 CS.configure CSconfig @16:09:34.88 PC.reconfigure PCconfig @16:09:35.22 PC.hold @16:09:35.31 PC.configure PCconfig @16:09:35.39 PS.configure PSconfig @16:09:35.49 Forearm.reconfigure LooseForearm @16:09:35.53 Forearm.hold @16:09:35.58 Forearm.configure LooseForearm @16:09:35.66 Elbow.reconfigure LooseElbow @16:09:35.70 Elbow.hold @16:09:35.75 Elbow.configure LooseElbow @16:09:35.85 SC.reconfigure SCloose @16:09:35.89 SC.hold @16:09:35.95 SC.configure SCloose @16:09:36.03 SE.reconfigure SEconfig @16:09:36.07 SE.hold @16:09:36.12 SE.configure SEconfig @16:09:36.16 CH.coast @16:09:36.20 CH.configure CHconfig @16:09:36.23 PH.coast @16:09:36.28 PH.configure PHconfig @16:09:36.32 PCR.off @16:09:36.34 PCR.pidtemp= 50,0.1,0,300,25,25 @16:09:36.37 PCR.caltemp= 2.7073,0.0373,0 @16:09:36.40 All dwarf controllers running firmware version 3.75 @16:09:36.51 Cleanly exited at 16:08:35CEST14-Jul-18 @16:09:36.54 Loading /home/esp/esp2/mission/stdMER2017.rb mission @16:09:37.06 AFV.select :carrierSolution @16:09:37.16 delay 2 seconds @16:09:39.19 AS.seek 0.5ml @16:10:08.27 delay 1 second @16:10:09.29 AFV.select :holdingCoil @16:10:09.38 ATV.dial :extSrc @16:10:10.84 AS.reconfigure ASslow1 @16:10:10.89 AS.seek 0.53ml @16:10:17.19 delay 5 seconds @16:10:22.26 AS.reconfigure ASslow2 @16:10:22.30 AS.seek 0.54ml @16:10:32.66 delay 20 seconds @16:10:52.73 AS.reconfigure ASslow1 @16:10:52.77 AS.seek 0.57ml @16:10:59.11 delay 5 seconds @16:11:04.19 AS.reconfigure ASslow2 @16:11:04.23 AS.seek 0.58ml @16:11:14.46 delay 20 seconds @16:11:34.53 AS.reconfigure ASslow1 @16:11:34.57 AS.seek 0.61ml @16:11:40.85 delay 5 seconds @16:11:45.92 AS.reconfigure ASslow2 @16:11:45.96 AS.seek 0.62ml @16:11:56.24 delay 20 seconds @16:12:16.31 AS.reconfigure ASslow1 @16:12:16.35 AS.seek 0.65ml @16:12:22.65 delay 5 seconds @16:12:27.73 AS.reconfigure ASslow2 @16:12:27.77 AS.seek 0.66ml @16:12:38.15 delay 20 seconds @16:12:58.17 AS.reconfigure ASslow1 @16:12:58.22 AS.seek 0.705ml @16:13:07.55 delay 10 seconds @16:13:17.57 ATV.dial :air1 @16:13:19.27 AS.seek 0.725ml @16:13:23.55 delay 3 seconds @16:13:26.57 ATV.dial :waste @16:13:27.21 AS.reconfigure ASslow0 @16:13:27.25 AS.seek :empty @16:14:40.67 Setting up PCR for Bb -- writing /var/log/esp/Bb18jul1416h.pcr @16:14:40.70 Building PCR Slug from extSrc with MM3 mix, PP5 primer @16:14:40.71 Priming with carrier @16:14:40.72 ATV.dial ARV @16:14:42.19 ARV.dial :carrier @16:14:42.53 AS.reconfigure ASnorm @16:14:42.58 AS.seek 0.499ml @16:15:08.14 AFV.select :carrierSolution @16:15:08.25 delay 2 seconds @16:15:10.27 AS.reconfigure ASconfig @16:15:10.32 AS.seek 1ml @16:15:39.37 delay 1 second @16:15:40.39 AFV.select :holdingCoil @16:15:40.49 delay 1 second @16:15:41.51 ATV.dial :waste @16:15:43.15 AS.reconfigure ASnorm @16:15:43.19 AS.seek :empty @16:16:33.92 AFV.select :carrierSolution @16:16:34.03 delay 2 seconds @16:16:36.05 AS.reconfigure ASconfig @16:16:36.10 AS.seek 0.5ml @16:17:05.16 delay 1 second @16:17:06.18 AFV.select :holdingCoil @16:17:06.28 Building Slug @16:17:06.29 ATV.dial :ARV @16:17:08.01 delay 2 seconds @16:17:10.03 ARV.dial :air1 @16:17:10.41 delay 2 seconds @16:17:12.48 AS.reconfigure ASslow2 @16:17:12.52 AS.seek 0.506ml @16:17:18.84 delay 2 seconds @16:17:20.86 ARV.dial :MM3 @16:17:21.93 delay 2 seconds @16:17:23.95 Adding MM3 @16:17:24.02 AS.seek 0.524ml @16:17:42.49 delay 25 seconds @16:18:07.51 ARV.dial :PP5 @16:18:07.90 Adding PP5 @16:18:07.91 delay 2 seconds @16:18:09.98 AS.seek 0.53ml @16:18:16.19 delay 10 seconds @16:18:26.22 ARV.dial :air3 @16:18:26.62 adding ARV air @16:18:26.63 delay 2 seconds @16:18:28.70 AS.seek 0.536ml @16:18:35.09 delay 5 seconds @16:18:40.11 ARV.dial :carrier @16:18:41.40 delay 7 seconds @16:18:48.47 AS.seek 0.594ml @16:19:47.23 delay 5 seconds @16:19:52.25 ATV.dial :extSrc @16:19:52.79 delay 2 seconds @16:19:54.86 AS.seek 0.6ml @16:20:01.18 delay 4 seconds @16:20:05.20 ATV.dial ARV @16:20:05.74 delay 2 seconds @16:20:07.76 ARV.dial :carrier @16:20:07.77 delay 2 seconds @16:20:09.84 AS.seek 0.65ml @16:21:00.35 delay 4 seconds @16:21:04.37 ATV.dial PCR @16:21:05.13 ASV.dial :waste1 @16:21:05.21 ARV.dial :air1 @16:21:05.61 Positioning Slug by 0.324ml @16:21:05.68 AS.seek 0.326ml @16:26:31.92 delay 5 seconds @16:26:36.94 ATV.dial ARV @16:26:37.81 ASV.dial :PCRlock @16:26:38.20 delay 5 seconds @16:26:43.22 Running Bb Profile60C300 thermocycling @16:26:43.23 PCR.program Profile60C300 @16:26:43.36 Profile60C300 should complete in < 1:45:21 @16:26:43.37 PCR.run 2:16:41.25 @17:47:27.93 PCR.off @17:47:29.39 PCR for Bb completed -- closed /var/log/esp/Bb18jul1416h.pcr @17:47:29.45 Cleaning PCR @17:47:29.46 AFV.select :carrierSolution @17:47:29.56 delay 2 seconds @17:47:31.58 AS.reconfigure ASconfig @17:47:31.63 AS.seek 0.5ml @17:47:41.92 delay 1 second @17:47:42.95 AFV.select :holdingCoil @17:47:43.11 ASV.dial :waste1 @17:47:43.53 ATV.dial :air3 @17:47:44.80 AS.reconfigure ASslow1 @17:47:44.85 AS.seek 0.51ml @17:47:47.12 delay 2 seconds @17:47:49.14 ATV.dial :bleach @17:47:49.81 AS.seek 0.81ml @17:48:50.39 delay 3 seconds @17:48:53.42 ATV.dial :air3 @17:48:53.97 delay 3 seconds @17:48:57.04 AS.seek 0.835ml @17:49:02.33 delay 3 seconds @17:49:05.35 ATV.dial :bleach @17:49:05.95 AS.seek 0.82ml @17:49:09.25 delay 3 seconds @17:49:12.28 ARV.dial :waste @17:49:12.89 delay 3 seconds @17:49:15.91 ATV.dial :ARV @17:49:16.78 AS.reconfigure ASconfig @17:49:16.82 AS.seek 0.47ml @17:49:37.22 AFV.select :carrierSolution @17:49:37.33 delay 2 seconds @17:49:39.36 AS.seek 1ml @17:50:10.10 delay 1 second @17:50:11.13 AFV.select :holdingCoil @17:50:11.23 delay 1 second @17:50:12.25 AS.reconfigure ASfast @17:50:12.30 AS.seek 0ml @17:50:40.08 AFV.select :carrierSolution @17:50:40.19 delay 2 seconds @17:50:42.21 AS.reconfigure ASconfig @17:50:42.26 AS.seek 1ml @17:51:40.00 delay 1 second @17:51:41.02 AFV.select :holdingCoil @17:51:41.12 delay 1 second @17:51:42.14 AS.reconfigure ASfast @17:51:42.19 AS.seek 0ml @17:52:09.89 AFV.select :carrierSolution @17:52:10.00 delay 2 seconds @17:52:12.02 AS.reconfigure ASconfig @17:52:12.07 AS.seek 1ml @17:53:09.82 delay 1 second @17:53:10.85 AFV.select :holdingCoil @17:53:10.95 delay 1 second @17:53:11.97 AS.reconfigure ASfast @17:53:12.02 AS.seek 0ml @17:53:39.99 ARV.dial between carrier and air1 @17:53:40.50 ATV.dial :air3 @17:53:41.77 AS.reconfigure ASslow1 @17:53:41.81 AS.seek 0.01ml @17:53:44.55 delay 2 seconds @17:53:46.57 ATV.dial :bleach @17:53:47.18 AS.seek 0.81ml @17:56:28.31 delay 3 seconds @17:56:31.33 ATV.dial :air3 @17:56:31.87 delay 3 seconds @17:56:34.94 AS.seek 0.835ml @17:56:40.23 delay 3 seconds @17:56:43.25 ATV.dial :bleach @17:56:43.86 AS.seek 0.82ml @17:56:47.16 delay 3 seconds @17:56:50.18 ATV.dial PCR @17:56:50.59 AS.reconfigure ASconfig @17:56:50.63 AS.seek :empty @17:57:38.10 PCR.program :bleachProfile @17:57:38.15 PCR program should complete in < 2:45 @17:57:38.16 PCR.run 10:00 @18:00:39.99 PCR.off @18:00:40.01 AFV.select :carrierSolution @18:00:40.12 delay 2 seconds @18:00:42.15 AS.seek 1ml @18:01:39.95 delay 1 second @18:01:40.97 AFV.select :holdingCoil @18:01:41.07 delay 1 second @18:01:42.09 AS.reconfigure ASfast @18:01:42.14 AS.seek 0ml @18:02:09.80 delay 3 seconds @18:02:12.82 AFV.select :carrierSolution @18:02:12.92 delay 2 seconds @18:02:14.94 AS.reconfigure ASconfig @18:02:15.00 AS.seek 1ml @18:03:12.74 delay 1 second @18:03:13.76 AFV.select :holdingCoil @18:03:13.86 delay 1 second @18:03:14.88 AS.reconfigure ASfast @18:03:14.93 AS.seek 0ml @18:03:42.74 delay 3 seconds @18:03:45.76 AFV.select :carrierSolution @18:03:45.86 delay 2 seconds @18:03:47.88 AS.reconfigure ASconfig @18:03:47.94 AS.seek 1ml @18:04:45.67 delay 1 second @18:04:46.69 AFV.select :holdingCoil @18:04:46.79 delay 1 second @18:04:47.81 AS.reconfigure ASfast @18:04:47.86 AS.seek 0ml @18:05:15.63 delay 3 seconds @18:05:18.65 AFV.select :carrierSolution @18:05:18.75 delay 2 seconds @18:05:20.77 AS.reconfigure ASconfig @18:05:20.83 AS.seek 1ml @18:06:18.58 delay 1 second @18:06:19.60 AFV.select :holdingCoil @18:06:19.70 delay 1 second @18:06:20.72 AS.reconfigure ASfast @18:06:20.77 AS.seek 0ml @18:06:48.11 delay 3 seconds @18:06:51.13 AFV.select :carrierSolution @18:06:51.23 delay 2 seconds @18:06:53.25 AS.reconfigure ASconfig @18:06:53.30 AS.seek 1ml @18:07:51.05 delay 1 second @18:07:52.07 AFV.select :holdingCoil @18:07:52.16 delay 1 second @18:07:53.18 AS.reconfigure ASnorm @18:07:53.23 AS.seek 0ml @18:08:43.93
AFV.select :carrierSolution @18:08:44.03 delay 2 seconds @18:08:46.05 AS.reconfigure ASconfig @18:08:46.10 AS.seek 0.5ml @18:09:15.14 delay 1 second @18:09:16.16 AFV.select :holdingCoil @18:09:16.25 ATV.dial :air1 @18:09:17.72 AS.reconfigure ASslow2 @18:09:17.76 AS.seek 0.506ml @18:09:24.03 ATV.dial :extSrc @18:09:25.69 AS.seek 0.512ml @18:09:32.05 delay 5 seconds @18:09:37.07 ATV.dial :air1 @18:09:38.76 AS.reconfigure ASslow1 @18:09:38.80 AS.seek 0.542ml @18:09:45.10 ATV.dial :waste @18:09:45.64 AS.reconfigure ASconfig @18:09:45.68 AS.seek :empty @18:10:17.33 Setting up PCR for Bb -- writing /var/log/esp/Bb18jul1418h.pcr @18:10:17.35 Building PCR Slug from extSrc with MM3 mix, PP5 primer @18:10:17.37 Priming with carrier ATV.dial ARV @18:10:18.95 ARV.dial :carrier @18:10:19.29 AS.reconfigure ASnorm @18:10:19.34 AS.seek 0.499ml @18:10:44.90 AFV.select :carrierSolution @18:10:45.01 delay 2 seconds @18:10:47.03 AS.reconfigure ASconfig @18:10:47.07 AS.seek 1ml @18:11:16.14 delay 1 second @18:11:17.16 AFV.select :holdingCoil @18:11:17.26 delay 1 second @18:11:18.28 ATV.dial :waste @18:11:19.92 AS.reconfigure ASnorm @18:11:19.96 AS.seek :empty @18:12:10.70 AFV.select :carrierSolution @18:12:10.81 delay 2 seconds @18:12:12.83 AS.reconfigure ASconfig @18:12:12.88 AS.seek 0.5ml @18:12:41.97 delay 1 second @18:12:42.99 AFV.select :holdingCoil @18:12:43.09 Building Slug @18:12:43.10 ATV.dial :ARV @18:12:44.82 delay 2 seconds @18:12:46.84 ARV.dial :air1 @18:12:47.24 delay 2 seconds @18:12:49.31 AS.reconfigure ASslow2 @18:12:49.35 AS.seek 0.506ml @18:12:55.82 delay 2 seconds @18:12:57.84 ARV.dial :MM3 @18:12:58.91 delay 2 seconds @18:13:00.93 Adding MM3 @18:13:01.00 AS.seek 0.524ml @18:13:19.35 delay 25 seconds @18:13:44.37 ARV.dial :PP5 @18:13:44.75 Adding PP5 @18:13:44.76 delay 2 seconds @18:13:46.83 AS.seek 0.53ml @18:13:53.17 delay 10 seconds @18:14:03.21 ARV.dial :air3 @18:14:03.60 adding ARV air @18:14:03.61 delay 2 seconds @18:14:05.68 AS.seek 0.536ml @18:14:12.06 delay 5 seconds @18:14:17.09 ARV.dial :carrier @18:14:18.38 delay 7 seconds @18:14:25.86 AS.seek 0.594ml @18:15:24.36 delay 5 seconds @18:15:29.38 ATV.dial :extSrc @18:15:29.96 delay 2 seconds @18:15:32.03 AS.seek 0.6ml @18:15:38.27 delay 4 seconds @18:15:42.29 ATV.dial ARV @18:15:42.93 delay 2 seconds @18:15:44.95 ARV.dial :carrier @18:15:44.96 delay 2 seconds @18:15:47.03 AS.seek 0.65ml @18:16:37.47 delay 4 seconds @18:16:41.49 ATV.dial PCR @18:16:42.24 ASV.dial :waste1 @18:16:42.25 ARV.dial :air1 @18:16:42.65 Positioning Slug by 0.324ml @18:16:42.72 AS.seek 0.326ml @18:22:08.74 delay 5 seconds @18:22:13.76 ATV.dial ARV @18:22:14.63 ASV.dial :PCRlock @18:22:15.02 delay 5 seconds @18:22:20.04 Running Bb Profile60C300 thermocycling @18:22:20.05 PCR.program Profile60C300 @18:22:20.17 Profile60C300 should complete in < 1:45:21 @18:22:20.18 PCR.run 2:16:41.25 @19:43:10.18 PCR.off @19:43:11.68 PCR for Bb completed -- closed /var/log/esp/Bb18jul1418h.pcr @19:43:11.74 Cleaning PCR @19:43:11.75 AFV.select :carrierSolution @19:43:11.85 delay 2 seconds @19:43:13.87 AS.reconfigure ASconfig @19:43:13.92 AS.seek 0.5ml @19:43:24.23 delay 1 second @19:43:25.25 AFV.select :holdingCoil @19:43:25.35 ASV.dial :waste1 @19:43:25.74 ATV.dial :air3 @19:43:27.00 AS.reconfigure ASslow1 @19:43:27.04 AS.seek 0.51ml @19:43:29.32 delay 2 seconds @19:43:31.35 ATV.dial :bleach @19:43:31.96 AS.seek 0.81ml @19:44:32.56 delay 3 seconds @19:44:35.58 ATV.dial :air3 @19:44:36.10 delay 3 seconds @19:44:39.17 AS.seek 0.835ml @19:44:44.37 delay 3 seconds @19:44:47.39 ATV.dial :bleach @19:44:47.98 AS.seek 0.82ml @19:44:51.27 delay 3 seconds @19:44:54.30 ARV.dial :waste @19:44:54.93 delay 3 seconds @19:44:57.95 ATV.dial :ARV @19:44:58.92 AS.reconfigure ASconfig @19:44:58.96 AS.seek 0.47ml @19:45:19.33 AFV.select :carrierSolution @19:45:19.44 delay 2 seconds @19:45:21.46 AS.seek 1ml @19:45:52.59 delay 1 second @19:45:53.61 AFV.select :holdingCoil @19:45:53.71 delay 1 second @19:45:54.73 AS.reconfigure ASfast @19:45:54.78 AS.seek 0ml @19:46:22.47 AFV.select :carrierSolution @19:46:22.58 delay 2 seconds @19:46:24.60 AS.reconfigure ASconfig @19:46:24.65 AS.seek 1ml @19:47:22.43 delay 1 second @19:47:23.45 AFV.select :holdingCoil @19:47:23.55 delay 1 second @19:47:24.57 AS.reconfigure ASfast @19:47:24.62 AS.seek 0ml @19:47:52.28 AFV.select :carrierSolution @19:47:52.39 delay 2 seconds @19:47:54.41 AS.reconfigure ASconfig @19:47:54.46 AS.seek 1ml @19:48:52.21 delay 1 second @19:48:53.23 AFV.select :holdingCoil @19:48:53.33 delay 1 second @19:48:54.35 AS.reconfigure ASfast @19:48:54.40 AS.seek 0ml @19:49:21.98 ARV.dial between carrier and air1 @19:49:22.50 ATV.dial :air3 @19:49:23.76 AS.reconfigure ASslow1 @19:49:23.81 AS.seek 0.01ml @19:49:26.12 delay 2 seconds @19:49:28.14 ATV.dial :bleach @19:49:28.74 AS.seek 0.81ml @19:52:09.85 delay 3 seconds @19:52:12.87 ATV.dial :air3 @19:52:13.42 delay 3 seconds @19:52:16.49 AS.seek 0.835ml @19:52:21.71 delay 3 seconds @19:52:24.73 ATV.dial :bleach @19:52:25.34 AS.seek 0.82ml @19:52:28.62 delay 3 seconds @19:52:31.64 ATV.dial PCR @19:52:32.04 AS.reconfigure ASconfig @19:52:32.08 AS.seek :empty @19:53:19.56 PCR.program :bleachProfile @19:53:19.61 PCR program should complete in < 2:45 @19:53:19.62 PCR.run 10:00 @19:56:21.45 PCR.off @19:56:21.47 AFV.select :carrierSolution @19:56:21.57 delay 2 seconds @19:56:23.60 AS.seek 1ml @19:57:21.37 delay 1 second @19:57:22.39 AFV.select :holdingCoil @19:57:22.49 delay 1 second @19:57:23.51 AS.reconfigure ASfast @19:57:23.56 AS.seek 0ml @19:57:51.22 delay 3 seconds @19:57:54.24 AFV.select :carrierSolution @19:57:54.34 delay 2 seconds @19:57:56.36 AS.reconfigure ASconfig @19:57:56.41 AS.seek 1ml @19:58:54.20 delay 1 second @19:58:55.22 AFV.select :holdingCoil @19:58:55.32 delay 1 second @19:58:56.34 AS.reconfigure ASfast @19:58:56.39 AS.seek 0ml @19:59:24.33 delay 3 seconds @19:59:27.35 AFV.select :carrierSolution @19:59:27.45 delay 2 seconds @19:59:29.47 AS.reconfigure ASconfig @19:59:29.53 AS.seek 1ml @20:00:27.31 delay 1 second @20:00:28.33 AFV.select :holdingCoil @20:00:28.43 delay 1 second @20:00:29.45 AS.reconfigure ASfast @20:00:29.50 AS.seek 0ml @20:00:57.20 delay 3 seconds @20:01:00.22 AFV.select :carrierSolution @20:01:00.32 delay 2 seconds @20:01:02.34 AS.reconfigure ASconfig @20:01:02.39 AS.seek 1ml @20:02:00.12 delay 1 second @20:02:01.14 AFV.select :holdingCoil @20:02:01.23 delay 1 second @20:02:02.25 AS.reconfigure ASfast @20:02:02.30 AS.seek 0ml @20:02:29.94 delay 3 seconds @20:02:32.96 AFV.select :carrierSolution @20:02:33.06 delay 2 seconds @20:02:35.08 AS.reconfigure ASconfig @20:02:35.14 AS.seek 1ml @20:03:32.91 delay 1 second @20:03:33.94 AFV.select :holdingCoil @20:03:34.03 delay 1 second @20:03:35.05 AS.reconfigure ASnorm @20:03:35.10 AS.seek 0ml @20:04:25.81
AFV.select :carrierSolution @20:04:25.91 delay 2 seconds @20:04:27.93 AS.reconfigure ASconfig @20:04:27.98 AS.seek 0.5ml @20:04:57.04 delay 1 second @20:04:58.07 AFV.select :holdingCoil @20:04:58.17 ATV.dial :air1 @20:04:59.64 AS.reconfigure ASslow2 @20:04:59.68 AS.seek 0.51ml @20:05:10.08 delay 5 seconds @20:05:15.10 ATV.dial :extSrc @20:05:16.77 AS.seek 0.53ml @20:05:37.11 delay 10 seconds @20:05:47.13 ATV.dial :air1 @20:05:48.81 AS.reconfigure ASslow1 @20:05:48.85 AS.seek 0.56ml @20:05:55.17 ATV.dial :waste @20:05:55.72 AS.reconfigure ASconfig @20:05:55.76 AS.seek :empty @20:06:28.45 Setting up PCR for Bb -- writing /var/log/esp/Bb18jul1420h.pcr @20:06:28.47 Building PCR Slug from extSrc with MM3 mix, PP5 primer @20:06:28.48 Priming with carrier @20:06:28.49 ATV.dial ARV @20:06:30.05 ARV.dial :carrier @20:06:30.39 AS.reconfigure ASnorm @20:06:30.44 AS.seek 0.499ml @20:06:55.99 AFV.select :carrierSolution @20:06:56.10 delay 2 seconds @20:06:58.12 AS.reconfigure ASconfig @20:06:58.17 AS.seek 1ml @20:07:27.23 delay 1 second @20:07:28.25 AFV.select :holdingCoil @20:07:28.35 delay 1 second @20:07:29.37 ATV.dial :waste @20:07:31.01 AS.reconfigure ASnorm @20:07:31.05 AS.seek :empty @20:08:21.87 AFV.select :carrierSolution @20:08:21.97 delay 2 seconds @20:08:23.99 AS.reconfigure ASconfig @20:08:24.04 AS.seek 0.5ml @20:08:53.14 delay 1 second @20:08:54.16 AFV.select :holdingCoil @20:08:54.26 Building Slug @20:08:54.27 ATV.dial :ARV @20:08:56.00 delay 2 seconds @20:08:58.02 ARV.dial :air1 @20:08:58.41 delay 2 seconds @20:09:00.48 AS.reconfigure ASslow2 @20:09:00.52 AS.seek 0.506ml @20:09:07.17 delay 2 seconds @20:09:09.19 ARV.dial :MM3 @20:09:10.25 delay 2 seconds @20:09:12.27 Adding MM3 @20:09:12.33 AS.seek 0.524ml @20:09:30.58 delay 25 seconds @20:09:55.60 ARV.dial :PP5 @20:09:56.00 Adding PP5 @20:09:56.01 delay 2 seconds @20:09:58.08 AS.seek 0.53ml @20:10:04.30 delay 10 seconds @20:10:14.33 ARV.dial :air3 @20:10:14.70 adding ARV air @20:10:14.71 delay 2 seconds @20:10:16.78 AS.seek 0.536ml @20:10:22.97 delay 5 seconds @20:10:27.99 ARV.dial :carrier @20:10:29.28 delay 7 seconds @20:10:36.36 AS.seek 0.594ml @20:11:34.83 delay 5 seconds @20:11:39.85 ATV.dial :extSrc @20:11:40.39 delay 2 seconds @20:11:42.46 AS.seek 0.6ml @20:11:48.64 delay 4 seconds @20:11:52.66 ATV.dial ARV @20:11:53.30 delay 2 seconds @20:11:55.32 ARV.dial :carrier @20:11:55.33 delay 2 seconds @20:11:57.40 AS.seek 0.65ml @20:12:47.89 delay 4 seconds @20:12:51.91 ATV.dial PCR @20:12:52.66 ASV.dial :waste1 @20:12:52.67 ARV.dial :air1 @20:12:53.06 Positioning Slug by 0.324ml @20:12:53.13 AS.seek 0.326ml @20:18:19.13 delay 5 seconds @20:18:24.15 ATV.dial ARV @20:18:25.01 ASV.dial :PCRlock @20:18:25.41 delay 5 seconds @20:18:30.43 Running Bb Profile60C300 thermocycling @20:18:30.44 PCR.program Profile60C300 @20:18:30.56 Profile60C300 should complete in < 1:45:21 @20:18:30.57 PCR.run 2:16:41.25 @21:39:16.97 PCR.off @21:39:18.55 PCR for Bb completed -- closed /var/log/esp/Bb18jul1420h.pcr @21:39:18.61 Cleaning PCR @21:39:18.62 AFV.select :carrierSolution @21:39:18.72 delay 2 seconds @21:39:20.74 AS.reconfigure ASconfig @21:39:20.79 AS.seek 0.5ml @21:39:31.10 delay 1 second @21:39:32.12 AFV.select :holdingCoil @21:39:32.22 ASV.dial :waste1 @21:39:32.62 ATV.dial :air3 @21:39:33.87 AS.reconfigure ASslow1 @21:39:33.92 AS.seek 0.51ml @21:39:36.22 delay 2 seconds @21:39:38.24 ATV.dial :bleach @21:39:38.85 AS.seek 0.81ml @21:40:39.43 delay 3 seconds @21:40:42.45 ATV.dial :air3 @21:40:42.99 delay 3 seconds @21:40:46.06 AS.seek 0.835ml @21:40:51.28 delay 3 seconds @21:40:54.31 ATV.dial :bleach @21:40:54.91 AS.seek 0.82ml @21:40:58.19 delay 3 seconds @21:41:01.21 ARV.dial :waste @21:41:01.82 delay 3 seconds @21:41:04.84 ATV.dial :ARV @21:41:05.71 AS.reconfigure ASconfig @21:41:05.75 AS.seek 0.47ml @21:41:26.15 AFV.select :carrierSolution @21:41:26.26 delay 2 seconds @21:41:28.29 AS.seek 1ml @21:41:59.03 delay 1 second @21:42:00.06 AFV.select :holdingCoil @21:42:00.16 delay 1 second @21:42:01.18 AS.reconfigure ASfast @21:42:01.23 AS.seek 0ml @21:42:28.97 AFV.select :carrierSolution @21:42:29.08 delay 2 seconds @21:42:31.10 AS.reconfigure ASconfig @21:42:31.15 AS.seek 1ml @21:43:28.96 delay 1 second @21:43:29.98 AFV.select :holdingCoil @21:43:30.08 delay 1 second @21:43:31.10 AS.reconfigure ASfast @21:43:31.15 AS.seek 0ml @21:43:58.89 AFV.select :carrierSolution @21:43:59.00 delay 2 seconds @21:44:01.03 AS.reconfigure ASconfig @21:44:01.07 AS.seek 1ml @21:44:58.79 delay 1 second @21:44:59.82 AFV.select :holdingCoil @21:44:59.92 delay 1 second @21:45:00.94 AS.reconfigure ASfast @21:45:00.99 AS.seek 0ml @21:45:28.89 ARV.dial between carrier and air1 @21:45:29.40 ATV.dial :air3 @21:45:30.67 AS.reconfigure ASslow1 @21:45:30.72 AS.seek 0.01ml @21:45:33.04 delay 2 seconds @21:45:35.07 ATV.dial :bleach @21:45:35.68 AS.seek 0.81ml @21:48:16.80 delay 3 seconds @21:48:19.82 ATV.dial :air3 @21:48:20.35 delay 3 seconds @21:48:23.42 AS.seek 0.835ml @21:48:28.72 delay 3 seconds @21:48:31.74 ATV.dial :bleach @21:48:32.35 AS.seek 0.82ml @21:48:35.59 delay 3 seconds @21:48:38.62 ATV.dial PCR @21:48:39.01 AS.reconfigure ASconfig @21:48:39.05 AS.seek :empty @21:49:26.48 PCR.program :bleachProfile @21:49:26.53 PCR program should complete in < 2:45 @21:49:26.54 PCR.run 10:00 @21:52:28.37 PCR.off @21:52:28.39 AFV.select :carrierSolution @21:52:28.49 delay 2 seconds @21:52:30.52 AS.seek 1ml @21:53:28.33 delay 1 second @21:53:29.36 AFV.select :holdingCoil @21:53:29.46 delay 1 second @21:53:30.48 AS.reconfigure ASfast @21:53:30.91 AS.seek 0ml @21:53:58.68 delay 3 seconds @21:54:01.70 AFV.select :carrierSolution @21:54:01.80 delay 2 seconds @21:54:03.82 AS.reconfigure ASconfig @21:54:03.88 AS.seek 1ml @21:55:01.62 delay 1 second @21:55:02.64 AFV.select :holdingCoil @21:55:02.74 delay 1 second @21:55:03.76 AS.reconfigure ASfast @21:55:03.81 AS.seek 0ml @21:55:31.79 delay 3 seconds @21:55:34.81 AFV.select :carrierSolution @21:55:34.91 delay 2 seconds @21:55:36.93 AS.reconfigure ASconfig @21:55:36.98 AS.seek 1ml @21:56:34.73 delay 1 second @21:56:35.75 AFV.select :holdingCoil @21:56:35.85 delay 1 second @21:56:36.87 AS.reconfigure ASfast @21:56:36.92 AS.seek 0ml @21:57:04.76 delay 3 seconds @21:57:07.78 AFV.select :carrierSolution @21:57:07.88 delay 2 seconds @21:57:09.90 AS.reconfigure ASconfig @21:57:09.96 AS.seek 1ml @21:58:07.71 delay 1 second @21:58:08.73 AFV.select :holdingCoil @21:58:08.83 delay 1 second @21:58:09.85 AS.reconfigure ASfast @21:58:09.90 AS.seek 0ml @21:58:37.53 delay 3 seconds @21:58:40.55 AFV.select :carrierSolution @21:58:40.65 delay 2 seconds @21:58:42.67 AS.reconfigure ASconfig @21:58:42.72 AS.seek 1ml @21:59:40.51 delay 1 second @21:59:41.53 AFV.select :holdingCoil @21:59:41.63 delay 1 second @21:59:42.65 AS.reconfigure ASnorm @21:59:42.70 AS.seek 0ml @22:00:33.40
AFV.select :carrierSolution @22:00:33.50 delay 2 seconds @22:00:35.52 AS.reconfigure ASconfig @22:00:35.57 AS.seek 0.5ml @22:01:04.64 delay 1 second @22:01:05.66 AFV.select :holdingCoil @22:01:05.76 ATV.dial :air1 @22:01:07.23 AS.reconfigure ASslow2 @22:01:07.27 AS.seek 0.506ml @22:01:13.57 ATV.dial :extSrc @22:01:15.35 AS.seek 0.512ml @22:01:21.54 delay 5 seconds @22:01:26.56 ATV.dial :air1 @22:01:28.25 AS.reconfigure ASslow1 @22:01:28.29 AS.seek 0.542ml @22:01:34.61 ATV.dial :waste @22:01:35.14 AS.reconfigure ASconfig @22:01:35.18 AS.seek :empty @22:02:06.84 Setting up PCR for Bb -- writing /var/log/esp/Bb18jul1422h.pcr @22:02:06.86 Building PCR Slug from extSrc with MM3 mix, PP5 primer @22:02:06.87 Priming with carrier ATV.dial ARV @22:02:08.34 ARV.dial :carrier @22:02:08.67 AS.reconfigure ASnorm @22:02:08.72 AS.seek 0.499ml @22:02:34.28 AFV.select :carrierSolution @22:02:34.39 delay 2 seconds @22:02:36.41 AS.reconfigure ASconfig @22:02:36.46 AS.seek 1ml @22:03:05.51 delay 1 second @22:03:06.53 AFV.select :holdingCoil @22:03:06.63 delay 1 second @22:03:07.65 ATV.dial :waste @22:03:09.29 AS.reconfigure ASnorm @22:03:09.33 AS.seek :empty @22:04:00.08 AFV.select :carrierSolution @22:04:00.19 delay 2 seconds @22:04:02.21 AS.reconfigure ASconfig @22:04:02.26 AS.seek 0.5ml @22:04:31.34 delay 1 second @22:04:32.36 AFV.select :holdingCoil @22:04:32.46 Building Slug @22:04:32.47 ATV.dial :ARV @22:04:34.20 delay 2 seconds @22:04:36.22 ARV.dial :air1 @22:04:36.61 delay 2 seconds @22:04:38.68 AS.reconfigure ASslow2 @22:04:38.72 AS.seek 0.506ml @22:04:44.98 delay 2 seconds @22:04:47.00 ARV.dial :MM3 @22:04:48.06 delay 2 seconds @22:04:50.08 Adding MM3 @22:04:50.15 AS.seek 0.524ml @22:05:08.71 delay 25 seconds @22:05:33.73 ARV.dial :PP5 @22:05:34.12 Adding PP5 @22:05:34.13 delay 2 seconds @22:05:36.20 AS.seek 0.53ml @22:05:42.66 delay 10 seconds @22:05:52.70 ARV.dial :air3 @22:05:53.09 adding ARV air @22:05:53.10 delay 2 seconds @22:05:55.17 AS.seek 0.536ml @22:06:01.37 delay 5 seconds @22:06:06.39 ARV.dial :carrier @22:06:07.68 delay 7 seconds @22:06:14.75 AS.seek 0.594ml @22:07:13.23 delay 5 seconds @22:07:18.25 ATV.dial :extSrc @22:07:19.17 delay 2 seconds @22:07:21.24 AS.seek 0.6ml @22:07:27.48 delay 4 seconds @22:07:31.50 ATV.dial ARV @22:07:32.04 delay 2 seconds @22:07:34.06 ARV.dial :carrier @22:07:34.07 delay 2 seconds @22:07:36.14 AS.seek 0.65ml @22:08:26.57 delay 4 seconds @22:08:30.59 ATV.dial PCR @22:08:31.45 ASV.dial :waste1 @22:08:31.46 ARV.dial :air1 @22:08:31.87 Positioning Slug by 0.324ml @22:08:31.94 AS.seek 0.326ml @22:13:58.09 delay 5 seconds @22:14:03.11 ATV.dial ARV @22:14:03.87 ASV.dial :PCRlock @22:14:04.26 delay 5 seconds @22:14:09.28 Running Bb Profile60C300 thermocycling @22:14:09.29 PCR.program Profile60C300 @22:14:09.41 Profile60C300 should complete in < 1:45:21 @22:14:09.42 PCR.run 2:16:41.25 @23:34:46.58 PCR.off @23:34:48.07 PCR for Bb completed -- closed /var/log/esp/Bb18jul1422h.pcr @23:34:48.14 Cleaning PCR @23:34:48.15 AFV.select :carrierSolution @23:34:48.25 delay 2 seconds @23:34:50.27 AS.reconfigure ASconfig @23:34:50.32 AS.seek 0.5ml @23:35:00.62 delay 1 second @23:35:01.65 AFV.select :holdingCoil @23:35:01.75 ASV.dial :waste1 @23:35:02.14 ATV.dial :air3 @23:35:03.41 AS.reconfigure ASslow1 @23:35:03.45 AS.seek 0.51ml @23:35:05.72 delay 2 seconds @23:35:07.74 ATV.dial :bleach @23:35:08.33 AS.seek 0.81ml @23:36:08.96 delay 3 seconds @23:36:11.98 ATV.dial :air3 @23:36:12.53 delay 3 seconds @23:36:15.60 AS.seek 0.835ml @23:36:20.89 delay 3 seconds @23:36:23.91 ATV.dial :bleach @23:36:24.91 AS.seek 0.82ml @23:36:28.19 delay 3 seconds @23:36:31.21 ARV.dial :waste @23:36:31.81 delay 3 seconds @23:36:34.83 ATV.dial :ARV @23:36:35.71 AS.reconfigure ASconfig @23:36:35.76 AS.seek 0.47ml @23:36:56.16 AFV.select :carrierSolution @23:36:56.27 delay 2 seconds @23:36:58.30 AS.seek 1ml @23:37:29.03 delay 1 second @23:37:30.05 AFV.select :holdingCoil @23:37:30.15 delay 1 second @23:37:31.17 AS.reconfigure ASfast @23:37:31.22 AS.seek 0ml @23:37:58.99 AFV.select :carrierSolution @23:37:59.10 delay 2 seconds @23:38:01.13 AS.reconfigure ASconfig @23:38:01.17 AS.seek 1ml @23:38:58.92 delay 1 second @23:38:59.94 AFV.select :holdingCoil @23:39:00.04 delay 1 second @23:39:01.06 AS.reconfigure ASfast @23:39:01.11 AS.seek 0ml @23:39:28.90 AFV.select :carrierSolution @23:39:29.01 delay 2 seconds @23:39:31.03 AS.reconfigure ASconfig @23:39:31.08 AS.seek 1ml @23:40:28.82 delay 1 second @23:40:29.84 AFV.select :holdingCoil @23:40:29.94 delay 1 second @23:40:30.96 AS.reconfigure ASfast @23:40:31.01 AS.seek 0ml @23:40:58.88 ARV.dial between carrier and air1 @23:40:59.39 ATV.dial :air3 @23:41:00.66 AS.reconfigure ASslow1 @23:41:00.71 AS.seek 0.01ml @23:41:03.00 delay 2 seconds @23:41:05.02 ATV.dial :bleach @23:41:05.63 AS.seek 0.81ml @23:43:46.77 delay 3 seconds @23:43:49.79 ATV.dial :air3 @23:43:50.33 delay 3 seconds @23:43:53.40 AS.seek 0.835ml @23:43:58.75 delay 3 seconds @23:44:01.78 ATV.dial :bleach @23:44:02.39 AS.seek 0.82ml @23:44:05.71 delay 3 seconds @23:44:08.73 ATV.dial PCR @23:44:09.13 AS.reconfigure ASconfig @23:44:09.17 AS.seek :empty @23:44:56.63 PCR.program :bleachProfile @23:44:56.68 PCR program should complete in < 2:45 @23:44:56.69 PCR.run 10:00 @23:47:58.52 PCR.off @23:47:58.54 AFV.select :carrierSolution @23:47:58.64 delay 2 seconds @23:48:00.67 AS.seek 1ml @23:48:58.46 delay 1 second @23:48:59.48 AFV.select :holdingCoil @23:48:59.58 delay 1 second @23:49:00.60 AS.reconfigure ASfast @23:49:00.65 AS.seek 0ml @23:49:28.58 delay 3 seconds @23:49:31.60 AFV.select :carrierSolution @23:49:31.70 delay 2 seconds @23:49:33.72 AS.reconfigure ASconfig @23:49:33.78 AS.seek 1ml @23:50:31.51 delay 1 second @23:50:32.54 AFV.select :holdingCoil @23:50:32.64 delay 1 second @23:50:33.66 AS.reconfigure ASfast @23:50:33.71 AS.seek 0ml @23:51:01.49 delay 3 seconds @23:51:04.51 AFV.select :carrierSolution @23:51:04.61 delay 2 seconds @23:51:06.63 AS.reconfigure ASconfig @23:51:06.68 AS.seek 1ml @23:52:04.44 delay 1 second @23:52:05.46 AFV.select :holdingCoil @23:52:05.56 delay 1 second @23:52:06.58 AS.reconfigure ASfast @23:52:06.63 AS.seek 0ml @23:52:34.23 delay 3 seconds @23:52:37.25 AFV.select :carrierSolution @23:52:37.35 delay 2 seconds @23:52:39.37 AS.reconfigure ASconfig @23:52:39.43 AS.seek 1ml @23:53:37.18 delay 1 second @23:53:38.21 AFV.select :holdingCoil @23:53:38.31 delay 1 second @23:53:39.33 AS.reconfigure ASfast @23:53:39.38 AS.seek 0ml @23:54:06.91 delay 3 seconds @23:54:09.93 AFV.select :carrierSolution @23:54:10.03 delay 2 seconds @23:54:12.05 AS.reconfigure ASconfig @23:54:12.10 AS.seek 1ml @23:55:09.88 delay 1 second @23:55:10.90 AFV.select :holdingCoil @23:55:11.00 delay 1 second @23:55:12.02 AS.reconfigure ASnorm @23:55:12.07 AS.seek 0ml @23:56:02.78
AFV.select :carrierSolution @23:56:02.88 delay 2 seconds @23:56:05.28 AS.reconfigure ASconfig @23:56:05.33 AS.seek 0.5ml @23:56:34.37 delay 1 second @23:56:35.39 AFV.select :holdingCoil @23:56:35.49 ATV.dial :air1 @23:56:36.96 AS.reconfigure ASslow1 @23:56:37.00 AS.seek 0.52ml @23:56:41.29 delay 5 seconds @23:56:46.31 ATV.dial :extSrc @23:56:48.09 AS.reconfigure ASslow2 @23:56:48.14 AS.seek 0.545ml @23:57:13.49 delay 10 seconds @23:57:23.51 ATV.dial :air1 @23:57:25.18 AS.reconfigure ASslow1 @23:57:25.23 AS.seek 0.575ml @23:57:31.57 ATV.dial :waste @23:57:32.10 AS.reconfigure ASconfig @23:57:32.14 AS.seek :empty @23:58:05.68 Setting up PCR for Bb -- writing /var/log/esp/Bb18jul1423h.pcr @23:58:05.70 Building PCR Slug from extSrc with MM3 mix, PP5 primer @23:58:05.71 Priming with carrier @23:58:05.72 ATV.dial ARV @23:58:07.19 ARV.dial :carrier @23:58:07.54 AS.reconfigure ASnorm @23:58:07.58 AS.seek 0.499ml @23:58:33.12 AFV.select :carrierSolution @23:58:33.23 delay 2 seconds @23:58:35.25 AS.reconfigure ASconfig @23:58:35.30 AS.seek 1ml @23:59:04.37 delay 1 second @23:59:05.39 AFV.select :holdingCoil @23:59:05.49 delay 1 second @23:59:06.51 ATV.dial :waste @23:59:08.26 AS.reconfigure ASnorm @23:59:08.30 AS.seek :empty @23:59:59.01 AFV.select :carrierSolution @23:59:59.12 delay 2 seconds @00:00:01.14CEST15-Jul-18 AS.reconfigure ASconfig @00:00:01.19 AS.seek 0.5ml @00:00:30.29 delay 1 second @00:00:31.31 AFV.select :holdingCoil @00:00:31.41 Building Slug @00:00:31.42 ATV.dial :ARV @00:00:33.04 delay 2 seconds @00:00:35.06 ARV.dial :air1 @00:00:35.46 delay 2 seconds @00:00:37.53 AS.reconfigure ASslow2 @00:00:37.57 AS.seek 0.506ml @00:00:44.05 delay 2 seconds @00:00:46.07 ARV.dial :MM3 @00:00:47.13 delay 2 seconds @00:00:49.15 Adding MM3 @00:00:49.22 AS.seek 0.524ml @00:01:07.60 delay 25 seconds @00:01:32.62 ARV.dial :PP5 @00:01:33.01 Adding PP5 @00:01:33.02 delay 2 seconds @00:01:35.09 AS.seek 0.53ml @00:01:41.55 delay 10 seconds @00:01:51.58 ARV.dial :air3 @00:01:51.98 adding ARV air @00:01:51.99 delay 2 seconds @00:01:54.06 AS.seek 0.536ml @00:02:00.45 delay 5 seconds @00:02:05.48 ARV.dial :carrier @00:02:06.77 delay 7 seconds @00:02:13.85 AS.seek 0.594ml @00:03:12.48 delay 5 seconds @00:03:17.50 ATV.dial :extSrc @00:03:18.06 delay 2 seconds @00:03:20.13 AS.seek 0.6ml @00:03:26.47 delay 4 seconds @00:03:30.50 ATV.dial ARV @00:03:31.04 delay 2 seconds @00:03:33.06 ARV.dial :carrier @00:03:33.07 delay 2 seconds @00:03:35.14 AS.seek 0.65ml @00:04:25.59 delay 4 seconds @00:04:29.62 ATV.dial PCR @00:04:30.47 ASV.dial :waste1 @00:04:30.49 ARV.dial :air1 @00:04:30.91 Positioning Slug by 0.324ml @00:04:30.99 AS.seek 0.326ml @00:09:56.80 delay 5 seconds @00:10:01.83 ATV.dial ARV @00:10:02.58 ASV.dial :PCRlock @00:10:02.98 delay 5 seconds @00:10:08.00 Running Bb Profile60C300 thermocycling @00:10:08.01 PCR.program Profile60C300 @00:10:08.13 Profile60C300 should complete in < 1:45:21 @00:10:08.14 PCR.run 2:16:41.25 @01:30:56.72 PCR.off @01:30:58.24 PCR for Bb completed -- closed /var/log/esp/Bb18jul1423h.pcr @01:30:58.30 Cleaning PCR @01:30:58.31 AFV.select :carrierSolution @01:30:58.41 delay 2 seconds @01:31:00.43 AS.reconfigure ASconfig @01:31:00.48 AS.seek 0.5ml @01:31:10.77 delay 1 second @01:31:11.80 AFV.select :holdingCoil @01:31:11.90 ASV.dial :waste1 @01:31:12.30 ATV.dial :air3 @01:31:13.56 AS.reconfigure ASslow1 @01:31:13.61 AS.seek 0.51ml @01:31:15.86 delay 2 seconds @01:31:17.89 ATV.dial :bleach @01:31:18.50 AS.seek 0.81ml @01:32:19.09 delay 3 seconds @01:32:22.11 ATV.dial :air3 @01:32:22.65 delay 3 seconds @01:32:25.72 AS.seek 0.835ml @01:32:30.92 delay 3 seconds @01:32:33.95 ATV.dial :bleach @01:32:34.55 AS.seek 0.82ml @01:32:37.83 delay 3 seconds @01:32:40.86 ARV.dial :waste @01:32:41.47 delay 3 seconds @01:32:44.49 ATV.dial :ARV @01:32:45.36 AS.reconfigure ASconfig @01:32:45.40 AS.seek 0.47ml @01:33:05.77 AFV.select :carrierSolution @01:33:05.88 delay 2 seconds @01:33:07.90 AS.seek 1ml @01:33:38.69 delay 1 second @01:33:39.71 AFV.select :holdingCoil @01:33:39.81 delay 1 second @01:33:40.83 AS.reconfigure ASfast @01:33:40.88 AS.seek 0ml @01:34:08.63 AFV.select :carrierSolution @01:34:08.74 delay 2 seconds @01:34:10.76 AS.reconfigure ASconfig @01:34:10.81 AS.seek 1ml @01:35:08.56 delay 1 second @01:35:09.58 AFV.select :holdingCoil @01:35:09.68 delay 1 second @01:35:10.70 AS.reconfigure ASfast @01:35:10.75 AS.seek 0ml @01:35:38.74 AFV.select :carrierSolution @01:35:38.85 delay 2 seconds @01:35:40.87 AS.reconfigure ASconfig @01:35:40.92 AS.seek 1ml @01:36:38.65 delay 1 second @01:36:39.68 AFV.select :holdingCoil @01:36:39.78 delay 1 second @01:36:40.80 AS.reconfigure ASfast @01:36:40.85 AS.seek 0ml @01:37:08.43 ARV.dial between carrier and air1 @01:37:09.31 ATV.dial :air3 @01:37:10.58 AS.reconfigure ASslow1 @01:37:10.63 AS.seek 0.01ml @01:37:12.95 delay 2 seconds @01:37:14.97 ATV.dial :bleach @01:37:15.58 AS.seek 0.81ml @01:39:56.69 delay 3 seconds @01:39:59.71 ATV.dial :air3 @01:40:00.25 delay 3 seconds @01:40:03.32 AS.seek 0.835ml @01:40:08.54 delay 3 seconds @01:40:11.56 ATV.dial :bleach @01:40:12.17 AS.seek 0.82ml @01:40:15.44 delay 3 seconds @01:40:18.47 ATV.dial PCR @01:40:18.86 AS.reconfigure ASconfig @01:40:18.90 AS.seek :empty @01:41:06.37 PCR.program :bleachProfile @01:41:06.42 PCR program should complete in < 2:45 @01:41:06.43 PCR.run 10:00 @01:44:08.26 PCR.off @01:44:08.29 AFV.select :carrierSolution @01:44:08.39 delay 2 seconds @01:44:10.42 AS.seek 1ml @01:45:08.25 delay 1 second @01:45:09.27 AFV.select :holdingCoil @01:45:09.37 delay 1 second @01:45:10.39 AS.reconfigure ASfast @01:45:10.44 AS.seek 0ml @01:45:38.05 delay 3 seconds @01:45:41.07 AFV.select :carrierSolution @01:45:41.17 delay 2 seconds @01:45:43.19 AS.reconfigure ASconfig @01:45:43.24 AS.seek 1ml @01:46:41.03 delay 1 second @01:46:42.05 AFV.select :holdingCoil @01:46:42.15 delay 1 second @01:46:43.17 AS.reconfigure ASfast @01:46:43.22 AS.seek 0ml @01:47:11.10 delay 3 seconds @01:47:14.12 AFV.select :carrierSolution @01:47:14.22 delay 2 seconds @01:47:16.24 AS.reconfigure ASconfig @01:47:16.29 AS.seek 1ml @01:48:14.03 delay 1 second @01:48:15.05 AFV.select :holdingCoil @01:48:15.15 delay 1 second @01:48:16.17 AS.reconfigure ASfast @01:48:16.22 AS.seek 0ml @01:48:43.80 delay 3 seconds @01:48:46.82 AFV.select :carrierSolution @01:48:46.92 delay 2 seconds @01:48:48.94 AS.reconfigure ASconfig @01:48:49.00 AS.seek 1ml @01:49:46.75 delay 1 second @01:49:47.77 AFV.select :holdingCoil @01:49:47.87 delay 1 second @01:49:48.89 AS.reconfigure ASfast @01:49:48.94 AS.seek 0ml @01:50:16.50 delay 3 seconds @01:50:19.52 AFV.select :carrierSolution @01:50:19.62 delay 2 seconds @01:50:21.64 AS.reconfigure ASconfig @01:50:21.69 AS.seek 1ml @01:51:19.42 delay 1 second @01:51:20.44 AFV.select :holdingCoil @01:51:20.54 delay 1 second @01:51:21.56 AS.reconfigure ASnorm @01:51:21.61 AS.seek 0ml @01:52:12.31
AFV.select :carrierSolution @01:52:12.41 delay 2 seconds @01:52:14.43 AS.reconfigure ASconfig @01:52:14.48 AS.seek 0.5ml @01:52:43.52 delay 1 second @01:52:44.55 AFV.select :holdingCoil @01:52:44.64 ATV.dial :air1 @01:52:46.12 AS.reconfigure ASslow2 @01:52:46.16 AS.seek 0.506ml @01:52:52.59 ATV.dial :extSrc @01:52:54.27 AS.seek 0.512ml @01:53:00.63 delay 5 seconds @01:53:05.66 ATV.dial :air1 @01:53:07.37 AS.reconfigure ASslow1 @01:53:07.41 AS.seek 0.542ml @01:53:13.70 ATV.dial :waste @01:53:14.25 AS.reconfigure ASconfig @01:53:14.29 AS.seek :empty @01:53:45.94 Setting up PCR for Bb -- writing /var/log/esp/Bb18jul1501h.pcr @01:53:45.96 Building PCR Slug from extSrc with MM3 mix, PP5 primer @01:53:45.97 Priming with carrier @01:53:45.98 ATV.dial ARV @01:53:47.54 ARV.dial :carrier @01:53:47.88 AS.reconfigure ASnorm @01:53:47.93 AS.seek 0.499ml @01:54:13.43 AFV.select :carrierSolution @01:54:13.54 delay 2 seconds @01:54:15.56 AS.reconfigure ASconfig @01:54:15.61 AS.seek 1ml @01:54:45.02 delay 1 second @01:54:46.04 AFV.select :holdingCoil @01:54:46.14 delay 1 second @01:54:47.16 ATV.dial :waste @01:54:48.80 AS.reconfigure ASnorm @01:54:48.84 AS.seek :empty @01:55:39.57 AFV.select :carrierSolution @01:55:39.68 delay 2 seconds @01:55:41.70 AS.reconfigure ASconfig @01:55:41.75 AS.seek 0.5ml @01:56:10.82 delay 1 second @01:56:11.84 AFV.select :holdingCoil @01:56:11.94 Building Slug @01:56:11.95 ATV.dial :ARV @01:56:13.58 delay 2 seconds @01:56:15.60 ARV.dial :air1 @01:56:16.00 delay 2 seconds @01:56:18.07 AS.reconfigure ASslow2 @01:56:18.11 AS.seek 0.506ml @01:56:24.50 delay 2 seconds @01:56:26.52 ARV.dial :MM3 @01:56:27.58 delay 2 seconds @01:56:29.60 Adding MM3 @01:56:29.67 AS.seek 0.524ml @01:56:48.14 delay 25 seconds @01:57:13.16 ARV.dial :PP5 @01:57:13.55 Adding PP5 @01:57:13.56 delay 2 seconds @01:57:15.63 AS.seek 0.53ml @01:57:21.79 delay 10 seconds @01:57:31.82 ARV.dial :air3 @01:57:32.21 adding ARV air @01:57:32.22 delay 2 seconds @01:57:34.29 AS.seek 0.536ml @01:57:40.66 delay 5 seconds @01:57:45.68 ARV.dial :carrier @01:57:46.97 delay 7 seconds @01:57:54.05 AS.seek 0.594ml @01:58:52.71 delay 5 seconds @01:58:57.73 ATV.dial :extSrc @01:58:58.29 delay 2 seconds @01:59:00.36 AS.seek 0.6ml @01:59:06.83 delay 4 seconds @01:59:10.85 ATV.dial ARV @01:59:11.40 delay 2 seconds @01:59:13.42 ARV.dial :carrier @01:59:13.43 delay 2 seconds @01:59:15.50 AS.seek 0.65ml @02:00:06.05 delay 4 seconds @02:00:10.07 ATV.dial PCR @02:00:10.81 ASV.dial :waste1 @02:00:10.83 ARV.dial :air1 @02:00:11.23 Positioning Slug by 0.324ml @02:00:11.30 AS.seek 0.326ml @02:05:37.30 delay 5 seconds @02:05:42.32 ATV.dial ARV @02:05:43.07 ASV.dial :PCRlock @02:05:43.48 delay 5 seconds @02:05:48.50 Running Bb Profile60C300 thermocycling @02:05:48.51 PCR.program Profile60C300 @02:05:48.63 Profile60C300 should complete in < 1:45:21 @02:05:48.64 PCR.run 2:16:41.25 @03:26:26.32 PCR.off @03:26:27.82 PCR for Bb completed -- closed /var/log/esp/Bb18jul1501h.pcr @03:26:27.88 Cleaning PCR @03:26:27.89 AFV.select :carrierSolution @03:26:27.99 delay 2 seconds @03:26:30.01 AS.reconfigure ASconfig @03:26:30.06 AS.seek 0.5ml @03:26:40.37 delay 1 second @03:26:41.39 AFV.select :holdingCoil @03:26:41.49 ASV.dial :waste1 @03:26:41.88 ATV.dial :air3 @03:26:43.15 AS.reconfigure ASslow1 @03:26:43.20 AS.seek 0.51ml @03:26:45.46 delay 2 seconds @03:26:47.49 ATV.dial :bleach @03:26:48.10 AS.seek 0.81ml @03:27:48.81 delay 3 seconds @03:27:51.83 ATV.dial :air3 @03:27:52.35 delay 3 seconds @03:27:55.42 AS.seek 0.835ml @03:28:00.75 delay 3 seconds @03:28:03.77 ATV.dial :bleach @03:28:04.38 AS.seek 0.82ml @03:28:07.67 delay 3 seconds @03:28:10.70 ARV.dial :waste @03:28:11.31 delay 3 seconds @03:28:14.33 ATV.dial :ARV @03:28:15.20 AS.reconfigure ASconfig @03:28:15.25 AS.seek 0.47ml @03:28:35.64 AFV.select :carrierSolution @03:28:35.75 delay 2 seconds @03:28:37.78 AS.seek 1ml @03:29:08.50 delay 1 second @03:29:09.53 AFV.select :holdingCoil @03:29:09.63 delay 1 second @03:29:10.65 AS.reconfigure ASfast @03:29:10.70 AS.seek 0ml @03:29:38.25 AFV.select :carrierSolution @03:29:38.36 delay 2 seconds @03:29:40.38 AS.reconfigure ASconfig @03:29:40.43 AS.seek 1ml @03:30:38.20 delay 1 second @03:30:39.22 AFV.select :holdingCoil @03:30:39.32 delay 1 second @03:30:40.34 AS.reconfigure ASfast @03:30:40.39 AS.seek 0ml @03:31:08.06 AFV.select :carrierSolution @03:31:08.17 delay 2 seconds @03:31:10.19 AS.reconfigure ASconfig @03:31:10.24 AS.seek 1ml @03:32:07.97 delay 1 second @03:32:09.00 AFV.select :holdingCoil @03:32:09.10 delay 1 second @03:32:10.12 AS.reconfigure ASfast @03:32:10.17 AS.seek 0ml @03:32:37.86 ARV.dial between carrier and air1 @03:32:38.37 ATV.dial :air3 @03:32:39.64 AS.reconfigure ASslow1 @03:32:39.69 AS.seek 0.01ml @03:32:41.92 delay 2 seconds @03:32:43.94 ATV.dial :bleach @03:32:44.54 AS.seek 0.81ml @03:35:25.68 delay 3 seconds @03:35:28.70 ATV.dial :air3 @03:35:29.25 delay 3 seconds @03:35:32.32 AS.seek 0.835ml @03:35:37.58 delay 3 seconds @03:35:40.60 ATV.dial :bleach @03:35:41.20 AS.seek 0.82ml @03:35:44.50 delay 3 seconds @03:35:47.53 ATV.dial PCR @03:35:47.93 AS.reconfigure ASconfig @03:35:47.97 AS.seek :empty @03:36:35.44 PCR.program :bleachProfile @03:36:35.49 PCR program should complete in < 2:45 @03:36:35.50 PCR.run 10:00 @03:39:37.69 PCR.off @03:39:37.72 AFV.select :carrierSolution @03:39:37.82 delay 2 seconds @03:39:39.85 AS.seek 1ml @03:40:37.64 delay 1 second @03:40:38.66 AFV.select :holdingCoil @03:40:38.76 delay 1 second @03:40:39.78 AS.reconfigure ASfast @03:40:39.83 AS.seek 0ml @03:41:07.70 delay 3 seconds @03:41:10.72 AFV.select :carrierSolution @03:41:10.82 delay 2 seconds @03:41:12.84 AS.reconfigure ASconfig @03:41:12.89 AS.seek 1ml @03:42:10.70 delay 1 second @03:42:11.72 AFV.select :holdingCoil @03:42:11.82 delay 1 second @03:42:12.84 AS.reconfigure ASfast @03:42:12.89 AS.seek 0ml @03:42:40.54 delay 3 seconds @03:42:43.56 AFV.select :carrierSolution @03:42:43.66 delay 2 seconds @03:42:45.68 AS.reconfigure ASconfig @03:42:45.74 AS.seek 1ml @03:43:43.50 delay 1 second @03:43:44.52 AFV.select :holdingCoil @03:43:44.62 delay 1 second @03:43:45.64 AS.reconfigure ASfast @03:43:45.69 AS.seek 0ml @03:44:13.34 delay 3 seconds @03:44:16.36 AFV.select :carrierSolution @03:44:16.46 delay 2 seconds @03:44:18.48 AS.reconfigure ASconfig @03:44:18.54 AS.seek 1ml @03:45:16.29 delay 1 second @03:45:17.32 AFV.select :holdingCoil @03:45:17.42 delay 1 second @03:45:18.44 AS.reconfigure ASfast @03:45:18.49 AS.seek 0ml @03:45:46.00 delay 3 seconds @03:45:49.02 AFV.select :carrierSolution @03:45:49.12 delay 2 seconds @03:45:51.14 AS.reconfigure ASconfig @03:45:51.20 AS.seek 1ml @03:46:48.94 delay 1 second @03:46:49.97 AFV.select :holdingCoil @03:46:50.07 delay 1 second @03:46:51.09 AS.reconfigure ASnorm @03:46:51.14 AS.seek 0ml @03:47:41.84
AFV.select :carrierSolution @03:47:41.94 delay 2 seconds @03:47:43.96 AS.reconfigure ASconfig @03:47:44.01 AS.seek 0.5ml @03:48:13.06 delay 1 second @03:48:14.08 AFV.select :holdingCoil @03:48:14.18 ATV.dial :air1 @03:48:15.66 AS.reconfigure ASslow1 @03:48:15.70 AS.seek 0.52ml @03:48:20.01 delay 5 seconds @03:48:25.03 ATV.dial :extSrc @03:48:26.81 AS.reconfigure ASslow2 @03:48:26.85 AS.seek 0.538ml @03:48:45.16 delay 10 seconds @03:48:55.18 ATV.dial :air1 @03:48:56.86 AS.reconfigure ASslow1 @03:48:56.90 AS.seek 0.568ml @03:49:03.18 ATV.dial :waste @03:49:03.83 AS.reconfigure ASconfig @03:49:03.87 AS.seek :empty @03:49:36.86 deconEluateRecoveryLine extSrc @03:49:36.87 ATV.dial :waste @03:49:36.89 AS.reconfigure ASnorm @03:49:36.93 AS.seek 0ml @03:49:37.30 ATV.dial :air3 @03:49:37.97 AS.reconfigure ASslow1 @03:49:38.01 AS.seek 0.01ml @03:49:40.26 delay 2 seconds @03:49:42.28 ATV.dial :bleach @03:49:42.73 AS.seek 0.31ml @03:50:43.33 delay 3 seconds @03:50:46.35 ATV.dial :air3 @03:50:46.89 delay 3 seconds @03:50:49.96 AS.seek 0.335ml @03:50:55.24 delay 3 seconds @03:50:58.27 ATV.dial :bleach @03:50:58.86 AS.seek 0.32ml @03:51:02.16 delay 3 seconds @03:51:05.19 ATV.dial :extSrc @03:51:05.80 AS.seek 0ml @03:52:10.41 AFV.select :carrierSolution @03:52:10.52 delay 2 seconds @03:52:12.54 AS.reconfigure ASconfig @03:52:12.59 AS.seek 0.5ml @03:52:41.61 delay 1 second @03:52:42.64 AFV.select :holdingCoil @03:52:42.76 ATV.dial :extSrc @03:52:42.78 AS.reconfigure ASslow1 @03:52:42.82 AS.seek 0ml @03:54:23.63 AFV.select :carrierSolution @03:54:23.74 delay 2 seconds @03:54:25.76 AS.reconfigure ASconfig @03:54:25.81 AS.seek 0.5ml @03:54:54.83 delay 1 second @03:54:55.85 AFV.select :holdingCoil @03:54:55.95 ATV.dial :extSrc @03:54:55.96 AS.reconfigure ASslow1 @03:54:56.01 AS.seek 0ml @03:56:36.80 AFV.select :carrierSolution @03:56:36.91 delay 2 seconds @03:56:38.93 AS.reconfigure ASconfig @03:56:38.98 AS.seek 0.5ml @03:57:08.01 delay 1 second @03:57:09.03 AFV.select :holdingCoil @03:57:09.13 ATV.dial :extSrc @03:57:09.14 AS.reconfigure ASslow1 @03:57:09.19 AS.seek 0ml @03:58:49.99 AFV.select :carrierSolution @03:58:50.10 delay 2 seconds @03:58:52.12 AS.reconfigure ASconfig @03:58:52.54 AS.seek 0.5ml @03:59:21.58 delay 1 second @03:59:22.60 AFV.select :holdingCoil @03:59:22.70 ATV.dial :extSrc @03:59:22.72 AS.reconfigure ASslow1 @03:59:22.76 AS.seek 0ml @04:01:03.59 ATV.dial :air2 @04:01:04.92 AS.reconfigure ASslow0 @04:01:04.96 AS.seek 0.5ml @04:01:55.54 ATV.dial :extSrc @04:01:57.02 AS.seek 0.02ml @04:02:45.60 delay 1 second @04:02:46.62 ATV.dial :waste @04:02:47.91 AS.reconfigure ASconfig @04:02:47.95 AS.seek :empty @04:02:49.46 deconEluateRecoveryLine extSrc ATV.dial :waste @04:02:49.48 AS.reconfigure ASnorm @04:02:49.52 AS.seek 0ml @04:02:49.91 ATV.dial :air3 @04:02:50.76 AS.reconfigure ASslow1 @04:02:50.80 AS.seek 0.01ml @04:02:53.06 delay 2 seconds @04:02:55.08 ATV.dial :bleach @04:02:55.53 AS.seek 0.31ml @04:03:56.13 delay 3 seconds @04:03:59.15 ATV.dial :air3 @04:03:59.69 delay 3 seconds @04:04:02.76 AS.seek 0.335ml @04:04:08.11 delay 3 seconds @04:04:11.14 ATV.dial :bleach @04:04:11.75 AS.seek 0.32ml @04:04:15.02 delay 3 seconds @04:04:18.04 ATV.dial :extSrc @04:04:18.64 AS.seek 0ml @04:05:23.27 AFV.select :carrierSolution @04:05:23.38 delay 2 seconds @04:05:25.40 AS.reconfigure ASconfig @04:05:25.45 AS.seek 0.5ml @04:05:54.49 delay 1 second @04:05:55.51 AFV.select :holdingCoil @04:05:55.60 ATV.dial :extSrc @04:05:55.61 AS.reconfigure ASslow1 @04:05:55.66 AS.seek 0ml @04:07:36.41 AFV.select :carrierSolution @04:07:36.52 delay 2 seconds @04:07:38.54 AS.reconfigure ASconfig @04:07:38.59 AS.seek 0.5ml @04:08:07.61 delay 1 second @04:08:08.63 AFV.select :holdingCoil @04:08:08.73 ATV.dial :extSrc @04:08:08.75 AS.reconfigure ASslow1 @04:08:08.79 AS.seek 0ml @04:09:49.62 AFV.select :carrierSolution @04:09:49.73 delay 2 seconds @04:09:51.75 AS.reconfigure ASconfig @04:09:51.80 AS.seek 0.5ml @04:10:20.83 delay 1 second @04:10:21.85 AFV.select :holdingCoil @04:10:21.95 ATV.dial :extSrc @04:10:21.96 AS.reconfigure ASslow1 @04:10:22.01 AS.seek 0ml @04:12:02.81 AFV.select :carrierSolution @04:12:02.92 delay 2 seconds @04:12:04.94 AS.reconfigure ASconfig @04:12:04.99 AS.seek 0.5ml @04:12:34.02 delay 1 second @04:12:35.04 AFV.select :holdingCoil @04:12:35.14 ATV.dial :extSrc @04:12:35.15 AS.reconfigure ASslow1 @04:12:35.20 AS.seek 0ml @04:14:16.03 ATV.dial :air2 @04:14:17.34 AS.reconfigure ASslow0 @04:14:17.38 AS.seek 0.5ml @04:15:07.99 ATV.dial :extSrc @04:15:09.45 AS.seek 0.02ml @04:15:58.07 delay 1 second @04:15:59.09 ATV.dial :waste @04:16:00.37 AS.reconfigure ASconfig @04:16:00.41 AS.seek :empty @04:16:01.90 deconEluateRecoveryLine extSrc @04:16:01.91 ATV.dial :waste @04:16:01.93 AS.reconfigure ASnorm @04:16:01.97 AS.seek 0ml @04:16:02.35 ATV.dial :air3 @04:16:03.28 AS.reconfigure ASslow1 @04:16:03.32 AS.seek 0.01ml @04:16:05.63 delay 2 seconds @04:16:07.65 ATV.dial :bleach @04:16:08.09 AS.seek 0.31ml @04:17:08.66 delay 3 seconds @04:17:11.68 ATV.dial :air3 @04:17:12.22 delay 3 seconds @04:17:15.29 AS.seek 0.335ml @04:17:20.58 delay 3 seconds @04:17:23.60 ATV.dial :bleach @04:17:24.20 AS.seek 0.32ml @04:17:27.50 delay 3 seconds @04:17:30.53 ATV.dial :extSrc @04:17:31.15 AS.seek 0ml @04:18:35.78 AFV.select :carrierSolution @04:18:35.89 delay 2 seconds @04:18:37.91 AS.reconfigure ASconfig @04:18:37.96 AS.seek 0.5ml @04:19:06.99 delay 1 second @04:19:08.01 AFV.select :holdingCoil @04:19:08.11 ATV.dial :extSrc @04:19:08.12 AS.reconfigure ASslow1 @04:19:08.53 AS.seek 0ml @04:20:49.36 AFV.select :carrierSolution @04:20:49.47 delay 2 seconds @04:20:51.49 AS.reconfigure ASconfig @04:20:51.54 AS.seek 0.5ml @04:21:20.57 delay 1 second @04:21:21.59 AFV.select :holdingCoil @04:21:21.69 ATV.dial :extSrc @04:21:21.71 AS.reconfigure ASslow1 @04:21:21.75 AS.seek 0ml @04:23:02.56 AFV.select :carrierSolution @04:23:02.67 delay 2 seconds @04:23:04.69 AS.reconfigure ASconfig @04:23:04.74 AS.seek 0.5ml @04:23:33.77 delay 1 second @04:23:34.79 AFV.select :holdingCoil @04:23:34.89 ATV.dial :extSrc @04:23:34.91 AS.reconfigure ASslow1 @04:23:34.95 AS.seek 0ml @04:25:15.78 AFV.select :carrierSolution @04:25:15.89 delay 2 seconds @04:25:17.91 AS.reconfigure ASconfig @04:25:17.96 AS.seek 0.5ml @04:25:46.98 delay 1 second @04:25:48.00 AFV.select :holdingCoil @04:25:48.10 ATV.dial :extSrc @04:25:48.12 AS.reconfigure ASslow1 @04:25:48.16 AS.seek 0ml @04:27:28.97 ATV.dial :air2 @04:27:30.39 AS.reconfigure ASslow0 @04:27:30.43 AS.seek 0.5ml @04:28:21.03 ATV.dial :extSrc @04:28:22.51 AS.seek 0.02ml @04:29:11.12 delay 1 second @04:29:12.14 ATV.dial :waste @04:29:13.42 AS.reconfigure ASconfig @04:29:13.46 AS.seek :empty @17:16:42.59CEST16-Jul-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @17:16:44.66 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @17:16:44.91 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @17:16:50.90 Polling Can every 10:00 @17:16:52.66 SPE.coast @17:16:52.70 SPE.configure SPEconfig @17:16:52.79 AS.reconfigure ASconfig @17:16:52.83 AS.hold @17:16:52.89 AS.configure ASconfig @17:16:52.97 SS.reconfigure SSconfig @17:16:53.01 SS.hold @17:16:53.06 SS.configure SSconfig @17:16:53.14 CC.reconfigure CCconfig @17:16:53.18 CC.hold @17:16:53.24 CC.configure CCconfig @17:16:53.32 CS.reconfigure CSconfig @17:16:53.36 CS.hold @17:16:53.43 CS.configure CSconfig @17:16:53.51 PC.configure PCconfig @17:16:53.94 PS.configure PSconfig @17:16:54.08 Forearm.reconfigure LooseForearm @17:16:54.15 Forearm.hold @17:16:54.25 Forearm.configure LooseForearm @17:16:54.42 Elbow.reconfigure LooseElbow @17:16:54.49 Elbow.hold @17:16:54.59 Elbow.configure LooseElbow @17:16:54.79 SC.reconfigure SCloose @17:16:54.85 SC.hold @17:16:54.91 SC.configure SCloose @17:16:54.99 SE.reconfigure SEconfig @17:16:55.02 SE.hold @17:16:55.07 SE.configure SEconfig @17:16:55.11 CH.coast @17:16:55.16 CH.configure CHconfig @17:16:55.19 PH.coast @17:16:55.24 PH.configure PHconfig @17:16:55.27 PCR.off @17:16:55.30 PCR.pidtemp= 50,0.1,0,300,25,25 @17:16:55.33 PCR.caltemp= 2.7073,0.0373,0 @17:16:55.36 All dwarf controllers running firmware version 3.75 @17:16:55.48 Cleanly exited at 17:15:20CEST16-Jul-18 @17:16:55.54 Loading /home/esp/esp2/mission/stdMER2017.rb mission @17:16:56.04 PSV.dial :air @17:16:56.28 PS.reconfigure PSconfig @17:16:56.43 Session Begun @17:16:56.49 PS.home.seek 0ml @17:16:57.05
Forearm.seek :retract @17:16:57.13 Elbow.seek :hammer @17:16:57.29 Forearm.reconfigure TightForearm @17:16:57.35 Forearm.seek 5 + 350 counts @17:16:59.41 Forearm.seek :retract @17:17:00.57 PC.reconfigure PCconfig @17:17:00.64 PC.home.seek :open @17:17:05.82 PS.coast @17:17:09.19
AFV.select :carrierSolution @17:17:09.29 delay 2 seconds @17:17:11.32 AS.seek 0.5ml @17:17:40.43 delay 1 second @17:17:41.45 AFV.select :holdingCoil @17:17:41.56 ATV.dial :extSrc @17:17:43.03 AS.reconfigure ASslow1 @17:17:43.08 AS.seek 0.53ml @17:17:49.41 delay 5 seconds @17:17:54.49 AS.reconfigure ASslow2 @17:17:54.55 AS.seek 0.54ml @17:18:04.85 delay 20 seconds @17:18:24.92 AS.reconfigure ASslow1 @17:18:24.98 AS.seek 0.57ml @17:18:31.24 delay 5 seconds @17:18:36.36 AS.reconfigure ASslow2 @17:18:36.42 AS.seek 0.58ml @17:18:46.61 delay 20 seconds @17:19:06.68 AS.reconfigure ASslow1 @17:19:06.74 AS.seek 0.61ml @17:19:13.05 delay 5 seconds @17:19:18.13 AS.reconfigure ASslow2 @17:19:18.19 AS.seek 0.62ml @17:19:28.52 delay 20 seconds @17:19:48.59 AS.reconfigure ASslow1 @17:19:48.65 AS.seek 0.65ml @17:19:54.93 delay 5 seconds @17:20:00.01 AS.reconfigure ASslow2 @17:20:00.07 AS.seek 0.66ml @17:20:10.41 delay 20 seconds @17:20:30.44 AS.reconfigure ASslow1 @17:20:30.50 AS.seek 0.705ml @17:20:39.90 delay 10 seconds @17:20:49.92 ATV.dial :air1 @17:20:51.66 AS.seek 0.725ml @17:20:55.96 delay 3 seconds @17:20:58.98 ATV.dial :waste @17:20:59.54 AS.reconfigure ASslow0 @17:20:59.60 AS.seek :empty @17:22:13.05 Setting up PCR for Rp -- writing /var/log/esp/Rp18jul1617h.pcr @17:22:13.08 Building PCR Slug from extSrc with MM2 mix, PP3 primer @17:22:13.10 Priming with carrier @17:22:13.12 ATV.dial ARV @17:22:14.63 ARV.dial :carrier @17:22:14.99 AS.reconfigure ASnorm @17:22:15.05 AS.seek 0.499ml @17:22:40.63 AFV.select :carrierSolution @17:22:40.74 delay 2 seconds @17:22:42.77 AS.reconfigure ASconfig @17:22:42.82 AS.seek 1ml @17:23:11.90 delay 1 second @17:23:12.92 AFV.select :holdingCoil @17:23:13.02 delay 1 second @17:23:14.04 ATV.dial :waste @17:23:15.82 AS.reconfigure ASnorm @17:23:15.88 AS.seek :empty @17:24:06.63 AFV.select :carrierSolution @17:24:06.74 delay 2 seconds @17:24:08.77 AS.reconfigure ASconfig @17:24:08.82 AS.seek 0.5ml @17:24:37.93 delay 1 second @17:24:38.95 AFV.select :holdingCoil @17:24:39.06 Building Slug @17:24:39.07 ATV.dial :ARV @17:24:40.71 delay 2 seconds @17:24:42.73 ARV.dial :air1 @17:24:43.14 delay 2 seconds @17:24:45.21 AS.reconfigure ASslow2 @17:24:45.27 AS.seek 0.506ml @17:24:51.55 delay 2 seconds @17:24:53.57 ARV.dial :MM2 @17:24:55.10 delay 2 seconds @17:24:57.12 Adding MM2 @17:24:57.22 AS.seek 0.524ml @17:25:15.66 delay 25 seconds @17:25:40.68 ARV.dial :PP3 @17:25:41.09 Adding PP3 @17:25:41.10 delay 2 seconds @17:25:43.18 AS.seek 0.53ml @17:25:49.49 delay 10 seconds @17:25:59.52 ARV.dial :air2 @17:25:59.93 adding ARV air @17:25:59.94 delay 2 seconds @17:26:02.02 AS.seek 0.536ml @17:26:08.52 delay 5 seconds @17:26:13.54 ARV.dial :carrier @17:26:14.87 delay 7 seconds @17:26:21.96 AS.seek 0.594ml @17:27:20.89 delay 5 seconds @17:27:25.91 ATV.dial :extSrc @17:27:26.48 delay 2 seconds @17:27:28.56 AS.seek 0.6ml @17:27:34.95 delay 4 seconds @17:27:38.97 ATV.dial ARV @17:27:39.52 delay 2 seconds @17:27:41.54 ARV.dial :carrier @17:27:41.55 delay 2 seconds @17:27:43.64 AS.seek 0.65ml @17:28:34.27 delay 4 seconds @17:28:38.29 ATV.dial PCR @17:28:39.07 ASV.dial :waste1 @17:28:39.15 ARV.dial :air1 @17:28:39.56 Positioning Slug by 0.324ml @17:28:39.65 AS.seek 0.326ml @17:34:05.70 delay 5 seconds @17:34:10.72 ATV.dial ARV @17:34:11.51 ASV.dial :PCRlock @17:34:11.92 delay 5 seconds @17:34:16.94 Running Rp Profile60C300 thermocycling @17:34:16.96 PCR.program Profile60C300 @17:34:17.10 Profile60C300 should complete in < 1:45:21 @17:34:17.11 PCR.run 2:16:41.25 @18:55:04.74 PCR.off @18:55:06.26 PCR for Rp completed -- closed /var/log/esp/Rp18jul1617h.pcr @18:55:06.34 Cleaning PCR @18:55:06.35 AFV.select :carrierSolution @18:55:06.47 delay 2 seconds @18:55:08.50 AS.reconfigure ASconfig @18:55:08.55 AS.seek 0.5ml @18:55:18.86 delay 1 second @18:55:19.88 AFV.select :holdingCoil @18:55:19.99 ASV.dial :waste1 @18:55:20.39 ATV.dial :air3 @18:55:21.68 AS.reconfigure ASslow1 @18:55:21.74 AS.seek 0.51ml @18:55:24.06 delay 2 seconds @18:55:26.08 ATV.dial :bleach @18:55:26.70 AS.seek 0.81ml @18:56:27.36 delay 3 seconds @18:56:30.38 ATV.dial :air3 @18:56:30.94 delay 3 seconds @18:56:34.01 AS.seek 0.835ml @18:56:39.31 delay 3 seconds @18:56:42.33 ATV.dial :bleach @18:56:42.95 AS.seek 0.82ml @18:56:46.28 delay 3 seconds @18:56:49.30 ARV.dial :waste @18:56:49.94 delay 3 seconds @18:56:52.96 ATV.dial :ARV @18:56:53.87 AS.reconfigure ASconfig @18:56:53.93 AS.seek 0.47ml @18:57:14.34 AFV.select :carrierSolution @18:57:14.45 delay 2 seconds @18:57:16.87 AS.seek 1ml @18:57:47.63 delay 1 second @18:57:48.65 AFV.select :holdingCoil @18:57:48.76 delay 1 second @18:57:49.79 AS.reconfigure ASfast @18:57:49.84 AS.seek 0ml @18:58:17.59 AFV.select :carrierSolution @18:58:17.70 delay 2 seconds @18:58:19.73 AS.reconfigure ASconfig @18:58:19.78 AS.seek 1ml @18:59:17.53 delay 1 second @18:59:18.55 AFV.select :holdingCoil @18:59:18.65 delay 1 second @18:59:19.68 AS.reconfigure ASfast @18:59:19.74 AS.seek 0ml @18:59:47.33 AFV.select :carrierSolution @18:59:47.45 delay 2 seconds @18:59:49.48 AS.reconfigure ASconfig @18:59:49.53 AS.seek 1ml @19:00:47.31 delay 1 second @19:00:48.33 AFV.select :holdingCoil @19:00:48.43 delay 1 second @19:00:49.46 AS.reconfigure ASfast @19:00:49.51 AS.seek 0ml @19:01:17.14 ARV.dial between carrier and air1 @19:01:17.66 ATV.dial :air3 @19:01:18.97 AS.reconfigure ASslow1 @19:01:19.03 AS.seek 0.01ml @19:01:21.38 delay 2 seconds @19:01:23.40 ATV.dial :bleach @19:01:24.03 AS.seek 0.81ml @19:04:05.19 delay 3 seconds @19:04:08.21 ATV.dial :air3 @19:04:08.77 delay 3 seconds @19:04:11.84 AS.seek 0.835ml @19:04:17.15 delay 3 seconds @19:04:20.17 ATV.dial :bleach @19:04:20.78 AS.seek 0.82ml @19:04:24.10 delay 3 seconds @19:04:27.12 ATV.dial PCR @19:04:27.55 AS.reconfigure ASconfig @19:04:27.61 AS.seek :empty @19:05:15.10 PCR.program :bleachProfile @19:05:15.15 PCR program should complete in < 2:45 @19:05:15.16 PCR.run 10:00 @19:08:17.00 PCR.off @19:08:17.03 AFV.select :carrierSolution @19:08:17.15 delay 2 seconds @19:08:19.18 AS.seek 1ml @19:09:16.97 delay 1 second @19:09:17.99 AFV.select :holdingCoil @19:09:18.10 delay 1 second @19:09:19.13 AS.reconfigure ASfast @19:09:19.18 AS.seek 0ml @19:09:46.69 delay 3 seconds @19:09:49.71 AFV.select :carrierSolution @19:09:49.81 delay 2 seconds @19:09:51.84 AS.reconfigure ASconfig @19:09:51.90 AS.seek 1ml @19:10:49.68 delay 1 second @19:10:50.70 AFV.select :holdingCoil @19:10:50.81 delay 1 second @19:10:51.84 AS.reconfigure ASfast @19:10:51.89 AS.seek 0ml @19:11:19.38 delay 3 seconds @19:11:22.40 AFV.select :carrierSolution @19:11:22.50 delay 2 seconds @19:11:24.53 AS.reconfigure ASconfig @19:11:24.58 AS.seek 1ml @19:12:22.38 delay 1 second @19:12:23.40 AFV.select :holdingCoil @19:12:23.51 delay 1 second @19:12:24.54 AS.reconfigure ASfast @19:12:24.59 AS.seek 0ml @19:12:52.18 delay 3 seconds @19:12:55.20 AFV.select :carrierSolution @19:12:55.30 delay 2 seconds @19:12:57.33 AS.reconfigure ASconfig @19:12:57.39 AS.seek 1ml @19:13:55.16 delay 1 second @19:13:56.18 AFV.select :holdingCoil @19:13:56.28 delay 1 second @19:13:57.31 AS.reconfigure ASfast @19:13:57.36 AS.seek 0ml @19:14:24.90 delay 3 seconds @19:14:27.92 AFV.select :carrierSolution @19:14:28.02 delay 2 seconds @19:14:30.05 AS.reconfigure ASconfig @19:14:30.11 AS.seek 1ml @19:15:27.87 delay 1 second @19:15:28.89 AFV.select :holdingCoil @19:15:29.00 delay 1 second @19:15:30.03 AS.reconfigure ASnorm @19:15:30.08 AS.seek 0ml @19:16:20.78
AFV.select :carrierSolution @19:16:20.89 delay 2 seconds @19:16:22.92 AS.reconfigure ASconfig @19:16:22.97 AS.seek 0.5ml @19:16:51.99 delay 1 second @19:16:53.01 AFV.select :holdingCoil @19:16:53.12 ATV.dial :air1 @19:16:54.63 AS.reconfigure ASslow2 @19:16:54.69 AS.seek 0.506ml @19:17:00.95 ATV.dial :extSrc @19:17:02.66 AS.seek 0.512ml @19:17:09.01 delay 5 seconds @19:17:14.03 ATV.dial :air1 @19:17:15.76 AS.reconfigure ASslow1 @19:17:15.82 AS.seek 0.542ml @19:17:22.15 ATV.dial :waste @19:17:22.81 AS.reconfigure ASconfig @19:17:22.87 AS.seek :empty @19:17:54.55 Setting up PCR for Rp -- writing /var/log/esp/Rp18jul1619h.pcr @19:17:54.57 Building PCR Slug from extSrc with MM2 mix, PP3 primer @19:17:54.60 Priming with carrier @19:17:54.61 ATV.dial ARV @19:17:56.13 ARV.dial :carrier @19:17:56.49 AS.reconfigure ASnorm @19:17:56.55 AS.seek 0.499ml @19:18:22.10 AFV.select :carrierSolution @19:18:22.21 delay 2 seconds @19:18:24.24 AS.reconfigure ASconfig @19:18:24.29 AS.seek 1ml @19:18:53.37 delay 1 second @19:18:54.39 AFV.select :holdingCoil @19:18:54.49 delay 1 second @19:18:55.51 ATV.dial :waste @19:18:57.18 AS.reconfigure ASnorm @19:18:57.24 AS.seek :empty @19:19:48.01 AFV.select :carrierSolution @19:19:48.12 delay 2 seconds @19:19:50.15 AS.reconfigure ASconfig @19:19:50.21 AS.seek 0.5ml @19:20:19.32 delay 1 second @19:20:20.34 AFV.select :holdingCoil @19:20:20.44 Building Slug @19:20:20.46 ATV.dial :ARV @19:20:22.21 delay 2 seconds @19:20:24.23 ARV.dial :air1 @19:20:25.06 delay 2 seconds @19:20:27.13 AS.reconfigure ASslow2 @19:20:27.20 AS.seek 0.506ml @19:20:33.42 delay 2 seconds @19:20:35.44 ARV.dial :MM2 @19:20:36.98 delay 2 seconds @19:20:39.00 Adding MM2 @19:20:39.08 AS.seek 0.524ml @19:20:57.37 delay 25 seconds @19:21:22.39 ARV.dial :PP3 @19:21:22.81 Adding PP3 @19:21:22.82 delay 2 seconds @19:21:24.91 AS.seek 0.53ml @19:21:31.17 delay 10 seconds @19:21:41.20 ARV.dial :air2 @19:21:41.60 adding ARV air @19:21:41.61 delay 2 seconds @19:21:43.69 AS.seek 0.536ml @19:21:50.06 delay 5 seconds @19:21:55.08 ARV.dial :carrier @19:21:56.42 delay 7 seconds @19:22:03.51 AS.seek 0.594ml @19:23:02.04 delay 5 seconds @19:23:07.06 ATV.dial :extSrc @19:23:07.60 delay 2 seconds @19:23:09.67 AS.seek 0.6ml @19:23:16.12 delay 4 seconds @19:23:20.14 ATV.dial ARV @19:23:20.70 delay 2 seconds @19:23:22.72 ARV.dial :carrier @19:23:22.73 delay 2 seconds @19:23:24.81 AS.seek 0.65ml @19:24:15.36 delay 4 seconds @19:24:19.38 ATV.dial PCR @19:24:20.15 ASV.dial :waste1 @19:24:20.16 ARV.dial :air1 @19:24:20.57 Positioning Slug by 0.324ml @19:24:20.66 AS.seek 0.326ml @19:29:46.73 delay 5 seconds @19:29:51.75 ATV.dial ARV @19:29:52.62 ASV.dial :PCRlock @19:29:53.01 delay 5 seconds @19:29:58.03 Running Rp Profile60C300 thermocycling @19:29:58.04 PCR.program Profile60C300 @19:29:58.18 Profile60C300 should complete in < 1:45:21 @19:29:58.19 PCR.run 2:16:41.25 @20:50:36.15 PCR.off @20:50:37.65 PCR for Rp completed -- closed /var/log/esp/Rp18jul1619h.pcr @20:50:37.72 Cleaning PCR @20:50:37.74 AFV.select :carrierSolution @20:50:37.85 delay 2 seconds @20:50:39.88 AS.reconfigure ASconfig @20:50:39.93 AS.seek 0.5ml @20:50:50.20 delay 1 second @20:50:51.22 AFV.select :holdingCoil @20:50:51.32 ASV.dial :waste1 @20:50:51.72 ATV.dial :air3 @20:50:53.40 AS.reconfigure ASslow1 @20:50:53.46 AS.seek 0.51ml @20:50:55.74 delay 2 seconds @20:50:57.76 ATV.dial :bleach @20:50:58.40 AS.seek 0.81ml @20:51:59.02 delay 3 seconds @20:52:02.04 ATV.dial :air3 @20:52:02.60 delay 3 seconds @20:52:05.67 AS.seek 0.835ml @20:52:11.00 delay 3 seconds @20:52:14.02 ATV.dial :bleach @20:52:14.64 AS.seek 0.82ml @20:52:17.95 delay 3 seconds @20:52:20.97 ARV.dial :waste @20:52:21.61 delay 3 seconds @20:52:24.63 ATV.dial :ARV @20:52:25.55 AS.reconfigure ASconfig @20:52:25.61 AS.seek 0.47ml @20:52:46.00 AFV.select :carrierSolution @20:52:46.11 delay 2 seconds @20:52:48.14 AS.seek 1ml @20:53:18.89 delay 1 second @20:53:19.91 AFV.select :holdingCoil @20:53:20.02 delay 1 second @20:53:21.05 AS.reconfigure ASfast @20:53:21.11 AS.seek 0ml @20:53:48.69 AFV.select :carrierSolution @20:53:48.80 delay 2 seconds @20:53:50.83 AS.reconfigure ASconfig @20:53:50.88 AS.seek 1ml @20:54:48.64 delay 1 second @20:54:49.66 AFV.select :holdingCoil @20:54:49.76 delay 1 second @20:54:50.79 AS.reconfigure ASfast @20:54:50.85 AS.seek 0ml @20:55:18.66 AFV.select :carrierSolution @20:55:18.77 delay 2 seconds @20:55:20.80 AS.reconfigure ASconfig @20:55:20.85 AS.seek 1ml @20:56:18.63 delay 1 second @20:56:19.65 AFV.select :holdingCoil @20:56:19.75 delay 1 second @20:56:20.78 AS.reconfigure ASfast @20:56:20.83 AS.seek 0ml @20:56:48.44 ARV.dial between carrier and air1 @20:56:48.97 ATV.dial :air3 @20:56:50.27 AS.reconfigure ASslow1 @20:56:50.33 AS.seek 0.01ml @20:56:52.61 delay 2 seconds @20:56:54.63 ATV.dial :bleach @20:56:55.25 AS.seek 0.81ml @20:59:36.43 delay 3 seconds @20:59:39.45 ATV.dial :air3 @20:59:40.00 delay 3 seconds @20:59:43.07 AS.seek 0.835ml @20:59:48.30 delay 3 seconds @20:59:51.32 ATV.dial :bleach @20:59:51.94 AS.seek 0.82ml @20:59:55.24 delay 3 seconds @20:59:58.26 ATV.dial PCR @20:59:58.68 AS.reconfigure ASconfig @20:59:58.74 AS.seek :empty @21:00:46.21 PCR.program :bleachProfile @21:00:46.26 PCR program should complete in < 2:45 @21:00:46.28 PCR.run 10:00 @21:03:48.11 PCR.off @21:03:48.14 AFV.select :carrierSolution @21:03:48.25 delay 2 seconds @21:03:50.28 AS.seek 1ml @21:04:48.07 delay 1 second @21:04:49.09 AFV.select :holdingCoil @21:04:49.20 delay 1 second @21:04:50.23 AS.reconfigure ASfast @21:04:50.28 AS.seek 0ml @21:05:17.94 delay 3 seconds @21:05:20.96 AFV.select :carrierSolution @21:05:21.07 delay 2 seconds @21:05:23.10 AS.reconfigure ASconfig @21:05:23.16 AS.seek 1ml @21:06:20.93 delay 1 second @21:06:21.95 AFV.select :holdingCoil @21:06:22.06 delay 1 second @21:06:23.09 AS.reconfigure ASfast @21:06:23.14 AS.seek 0ml @21:06:50.87 delay 3 seconds @21:06:53.89 AFV.select :carrierSolution @21:06:54.00 delay 2 seconds @21:06:56.03 AS.reconfigure ASconfig @21:06:56.09 AS.seek 1ml @21:07:53.85 delay 1 second @21:07:54.87 AFV.select :holdingCoil @21:07:54.97 delay 1 second @21:07:56.00 AS.reconfigure ASfast @21:07:56.05 AS.seek 0ml @21:08:23.57 delay 3 seconds @21:08:26.59 AFV.select :carrierSolution @21:08:26.69 delay 2 seconds @21:08:28.72 AS.reconfigure ASconfig @21:08:28.78 AS.seek 1ml @21:09:26.51 delay 1 second @21:09:27.53 AFV.select :holdingCoil @21:09:27.64 delay 1 second @21:09:28.67 AS.reconfigure ASfast @21:09:28.72 AS.seek 0ml @21:09:56.41 delay 3 seconds @21:09:59.43 AFV.select :carrierSolution @21:09:59.53 delay 2 seconds @21:10:01.56 AS.reconfigure ASconfig @21:10:01.61 AS.seek 1ml @21:10:59.37 delay 1 second @21:11:00.39 AFV.select :holdingCoil @21:11:00.49 delay 1 second @21:11:01.52 AS.reconfigure ASnorm @21:11:01.57 AS.seek 0ml @21:11:52.27
AFV.select :carrierSolution @21:11:52.38 delay 2 seconds @21:11:54.41 AS.reconfigure ASconfig @21:11:54.47 AS.seek 0.5ml @21:12:23.53 delay 1 second @21:12:24.55 AFV.select :holdingCoil @21:12:24.66 ATV.dial :air1 @21:12:26.16 AS.reconfigure ASslow2 @21:12:26.22 AS.seek 0.51ml @21:12:36.65 delay 5 seconds @21:12:41.67 ATV.dial :extSrc @21:12:43.40 AS.seek 0.53ml @21:13:03.83 delay 10 seconds @21:13:13.85 ATV.dial :air1 @21:13:15.57 AS.reconfigure ASslow1 @21:13:16.03 AS.seek 0.56ml @21:13:22.24 ATV.dial :waste @21:13:22.81 AS.reconfigure ASconfig @21:13:22.87 AS.seek :empty @21:13:55.53 Setting up PCR for Rp -- writing /var/log/esp/Rp18jul1621h.pcr @21:13:55.56 Building PCR Slug from extSrc with MM2 mix, PP3 primer @21:13:55.58 Priming with carrier @21:13:55.60 ATV.dial ARV @21:13:57.12 ARV.dial :carrier @21:13:57.48 AS.reconfigure ASnorm @21:13:57.54 AS.seek 0.499ml @21:14:23.12 AFV.select :carrierSolution @21:14:23.23 delay 2 seconds @21:14:25.26 AS.reconfigure ASconfig @21:14:25.32 AS.seek 1ml @21:14:54.40 delay 1 second @21:14:55.42 AFV.select :holdingCoil @21:14:55.52 delay 1 second @21:14:56.54 ATV.dial :waste @21:14:58.31 AS.reconfigure ASnorm @21:14:58.37 AS.seek :empty @21:15:49.12 AFV.select :carrierSolution @21:15:49.24 delay 2 seconds @21:15:51.27 AS.reconfigure ASconfig @21:15:51.33 AS.seek 0.5ml @21:16:20.42 delay 1 second @21:16:21.44 AFV.select :holdingCoil @21:16:21.54 Building Slug @21:16:21.55 ATV.dial :ARV @21:16:23.20 delay 2 seconds @21:16:25.22 ARV.dial :air1 @21:16:25.62 delay 2 seconds @21:16:27.69 AS.reconfigure ASslow2 @21:16:27.75 AS.seek 0.506ml @21:16:34.03 delay 2 seconds @21:16:36.05 ARV.dial :MM2 @21:16:37.57 delay 2 seconds @21:16:39.59 Adding MM2 @21:16:39.67 AS.seek 0.524ml @21:16:57.92 delay 25 seconds @21:17:22.94 ARV.dial :PP3 @21:17:23.34 Adding PP3 @21:17:23.35 delay 2 seconds @21:17:25.43 AS.seek 0.53ml @21:17:31.87 delay 10 seconds @21:17:41.90 ARV.dial :air2 @21:17:42.29 adding ARV air @21:17:42.30 delay 2 seconds @21:17:44.38 AS.seek 0.536ml @21:17:50.68 delay 5 seconds @21:17:55.70 ARV.dial :carrier @21:17:57.03 delay 7 seconds @21:18:04.11 AS.seek 0.594ml @21:19:02.56 delay 5 seconds @21:19:07.58 ATV.dial :extSrc @21:19:08.23 delay 2 seconds @21:19:10.30 AS.seek 0.6ml @21:19:16.54 delay 4 seconds @21:19:20.56 ATV.dial ARV @21:19:21.12 delay 2 seconds @21:19:23.14 ARV.dial :carrier @21:19:23.15 delay 2 seconds @21:19:25.23 AS.seek 0.65ml @21:20:15.67 delay 4 seconds @21:20:19.69 ATV.dial PCR @21:20:20.47 ASV.dial :waste1 @21:20:20.48 ARV.dial :air1 @21:20:20.91 Positioning Slug by 0.324ml @21:20:21.00 AS.seek 0.326ml @21:25:47.02 delay 5 seconds @21:25:52.04 ATV.dial ARV @21:25:52.83 ASV.dial :PCRlock @21:25:53.23 delay 5 seconds @21:25:58.25 Running Rp Profile60C300 thermocycling @21:25:58.26 PCR.program Profile60C300 @21:25:58.40 Profile60C300 should complete in < 1:45:21 @21:25:58.41 PCR.run 2:16:41.25 @22:46:30.94 PCR.off @22:46:32.45 PCR for Rp completed -- closed /var/log/esp/Rp18jul1621h.pcr @22:46:32.53 Cleaning PCR @22:46:32.54 AFV.select :carrierSolution @22:46:32.65 delay 2 seconds @22:46:34.68 AS.reconfigure ASconfig @22:46:34.73 AS.seek 0.5ml @22:46:45.10 delay 1 second @22:46:46.12 AFV.select :holdingCoil @22:46:46.22 ASV.dial :waste1 @22:46:46.63 ATV.dial :air3 @22:46:47.94 AS.reconfigure ASslow1 @22:46:48.00 AS.seek 0.51ml @22:46:50.29 delay 2 seconds @22:46:52.31 ATV.dial :bleach @22:46:52.95 AS.seek 0.81ml @22:47:53.53 delay 3 seconds @22:47:56.55 ATV.dial :air3 @22:47:57.11 delay 3 seconds @22:48:00.18 AS.seek 0.835ml @22:48:05.48 delay 3 seconds @22:48:08.50 ATV.dial :bleach @22:48:09.13 AS.seek 0.82ml @22:48:12.42 delay 3 seconds @22:48:15.44 ARV.dial :waste @22:48:16.08 delay 3 seconds @22:48:19.10 ATV.dial :ARV @22:48:20.11 AS.reconfigure ASconfig @22:48:20.17 AS.seek 0.47ml @22:48:40.57 AFV.select :carrierSolution @22:48:40.68 delay 2 seconds @22:48:42.71 AS.seek 1ml @22:49:13.47 delay 1 second @22:49:14.49 AFV.select :holdingCoil @22:49:14.59 delay 1 second @22:49:15.62 AS.reconfigure ASfast @22:49:15.67 AS.seek 0ml @22:49:43.39 AFV.select :carrierSolution @22:49:43.50 delay 2 seconds @22:49:45.53 AS.reconfigure ASconfig @22:49:45.58 AS.seek 1ml @22:50:43.35 delay 1 second @22:50:44.37 AFV.select :holdingCoil @22:50:44.47 delay 1 second @22:50:45.50 AS.reconfigure ASfast @22:50:45.55 AS.seek 0ml @22:51:13.18 AFV.select :carrierSolution @22:51:13.29 delay 2 seconds @22:51:15.32 AS.reconfigure ASconfig @22:51:15.37 AS.seek 1ml @22:52:13.13 delay 1 second @22:52:14.15 AFV.select :holdingCoil @22:52:14.25 delay 1 second @22:52:15.28 AS.reconfigure ASfast @22:52:15.33 AS.seek 0ml @22:52:42.93 ARV.dial between carrier and air1 @22:52:43.46 ATV.dial :air3 @22:52:44.75 AS.reconfigure ASslow1 @22:52:44.81 AS.seek 0.01ml @22:52:47.11 delay 2 seconds @22:52:49.13 ATV.dial :bleach @22:52:49.77 AS.seek 0.81ml @22:55:30.91 delay 3 seconds @22:55:33.93 ATV.dial :air3 @22:55:34.49 delay 3 seconds @22:55:37.94 AS.seek 0.835ml @22:55:43.33 delay 3 seconds @22:55:46.35 ATV.dial :bleach @22:55:46.97 AS.seek 0.82ml @22:55:50.29 delay 3 seconds @22:55:53.31 ATV.dial PCR @22:55:53.74 AS.reconfigure ASconfig @22:55:53.80 AS.seek :empty @22:56:41.29 PCR.program :bleachProfile @22:56:41.35 PCR program should complete in < 2:45 @22:56:41.36 PCR.run 10:00 @22:59:43.20 PCR.off @22:59:43.23 AFV.select :carrierSolution @22:59:43.35 delay 2 seconds @22:59:45.38 AS.seek 1ml @23:00:43.23 delay 1 second @23:00:44.25 AFV.select :holdingCoil @23:00:44.35 delay 1 second @23:00:45.38 AS.reconfigure ASfast @23:00:45.44 AS.seek 0ml @23:01:13.09 delay 3 seconds @23:01:16.11 AFV.select :carrierSolution @23:01:16.21 delay 2 seconds @23:01:18.24 AS.reconfigure ASconfig @23:01:18.30 AS.seek 1ml @23:02:16.07 delay 1 second @23:02:17.09 AFV.select :holdingCoil @23:02:17.19 delay 1 second @23:02:18.22 AS.reconfigure ASfast @23:02:18.27 AS.seek 0ml @23:02:45.95 delay 3 seconds @23:02:48.97 AFV.select :carrierSolution @23:02:49.07 delay 2 seconds @23:02:51.10 AS.reconfigure ASconfig @23:02:51.16 AS.seek 1ml @23:03:48.95 delay 1 second @23:03:49.97 AFV.select :holdingCoil @23:03:50.07 delay 1 second @23:03:51.10 AS.reconfigure ASfast @23:03:51.15 AS.seek 0ml @23:04:18.57 delay 3 seconds @23:04:21.59 AFV.select :carrierSolution @23:04:21.69 delay 2 seconds @23:04:23.72 AS.reconfigure ASconfig @23:04:23.77 AS.seek 1ml @23:05:21.57 delay 1 second @23:05:22.59 AFV.select :holdingCoil @23:05:22.69 delay 1 second @23:05:23.72 AS.reconfigure ASfast @23:05:23.77 AS.seek 0ml @23:05:51.24 delay 3 seconds @23:05:54.26 AFV.select :carrierSolution @23:05:54.36 delay 2 seconds @23:05:56.39 AS.reconfigure ASconfig @23:05:56.45 AS.seek 1ml @23:06:54.26 delay 1 second @23:06:55.28 AFV.select :holdingCoil @23:06:55.38 delay 1 second @23:06:56.41 AS.reconfigure ASnorm @23:06:56.46 AS.seek 0ml @23:07:47.16
AFV.select :carrierSolution @23:07:47.27 delay 2 seconds @23:07:49.30 AS.reconfigure ASconfig @23:07:49.35 AS.seek 0.5ml @23:08:18.43 delay 1 second @23:08:19.45 AFV.select :holdingCoil @23:08:19.55 ATV.dial :air1 @23:08:21.06 AS.reconfigure ASslow2 @23:08:21.12 AS.seek 0.506ml @23:08:27.37 ATV.dial :extSrc @23:08:29.18 AS.seek 0.512ml @23:08:35.43 delay 5 seconds @23:08:40.45 ATV.dial :air1 @23:08:42.17 AS.reconfigure ASslow1 @23:08:42.22 AS.seek 0.542ml @23:08:48.54 ATV.dial :waste @23:08:49.11 AS.reconfigure ASconfig @23:08:49.17 AS.seek :empty @23:09:20.84 Setting up PCR for Rp -- writing /var/log/esp/Rp18jul1623h.pcr @23:09:20.86 Building PCR Slug from extSrc with MM2 mix, PP3 primer @23:09:20.88 Priming with carrier @23:09:20.90 ATV.dial ARV @23:09:22.42 ARV.dial :carrier @23:09:22.79 AS.reconfigure ASnorm @23:09:22.85 AS.seek 0.499ml @23:09:48.43 AFV.select :carrierSolution @23:09:48.54 delay 2 seconds @23:09:50.57 AS.reconfigure ASconfig @23:09:50.63 AS.seek 1ml @23:10:19.72 delay 1 second @23:10:20.74 AFV.select :holdingCoil @23:10:20.84 delay 1 second @23:10:21.86 ATV.dial :waste @23:10:23.54 AS.reconfigure ASnorm @23:10:23.60 AS.seek :empty @23:11:14.36 AFV.select :carrierSolution @23:11:14.47 delay 2 seconds @23:11:16.50 AS.reconfigure ASconfig @23:11:16.55 AS.seek 0.5ml @23:11:45.65 delay 1 second @23:11:46.67 AFV.select :holdingCoil @23:11:46.78 Building Slug @23:11:47.15 ATV.dial :ARV @23:11:48.92 delay 2 seconds @23:11:50.94 ARV.dial :air1 @23:11:51.36 delay 2 seconds @23:11:53.43 AS.reconfigure ASslow2 @23:11:53.50 AS.seek 0.506ml @23:11:59.75 delay 2 seconds @23:12:01.77 ARV.dial :MM2 @23:12:03.29 delay 2 seconds @23:12:05.31 Adding MM2 @23:12:05.41 AS.seek 0.524ml @23:12:23.72 delay 25 seconds @23:12:48.74 ARV.dial :PP3 @23:12:49.14 Adding PP3 @23:12:49.15 delay 2 seconds @23:12:51.23 AS.seek 0.53ml @23:12:57.47 delay 10 seconds @23:13:07.50 ARV.dial :air2 @23:13:07.90 adding ARV air @23:13:07.91 delay 2 seconds @23:13:09.99 AS.seek 0.536ml @23:13:16.34 delay 5 seconds @23:13:21.36 ARV.dial :carrier @23:13:22.69 delay 7 seconds @23:13:29.77 AS.seek 0.594ml @23:14:28.31 delay 5 seconds @23:14:33.33 ATV.dial :extSrc @23:14:33.94 delay 2 seconds @23:14:36.02 AS.seek 0.6ml @23:14:42.29 delay 4 seconds @23:14:46.31 ATV.dial ARV @23:14:46.97 delay 2 seconds @23:14:48.99 ARV.dial :carrier @23:14:49.00 delay 2 seconds @23:14:51.08 AS.seek 0.65ml @23:15:41.73 delay 4 seconds @23:15:45.75 ATV.dial PCR @23:15:46.51 ASV.dial :waste1 @23:15:46.52 ARV.dial :air1 @23:15:46.95 Positioning Slug by 0.324ml @23:15:47.04 AS.seek 0.326ml @23:21:13.16 delay 5 seconds @23:21:18.18 ATV.dial ARV @23:21:19.06 ASV.dial :PCRlock @23:21:19.47 delay 5 seconds @23:21:24.49 Running Rp Profile60C300 thermocycling @23:21:24.50 PCR.program Profile60C300 @23:21:24.65 Profile60C300 should complete in < 1:45:21 @23:21:24.66 PCR.run 2:16:41.25 @00:42:05.94CEST17-Jul-18 PCR.off @00:42:07.73 PCR for Rp completed -- closed /var/log/esp/Rp18jul1623h.pcr @00:42:07.80 Cleaning PCR @00:42:07.81 AFV.select :carrierSolution @00:42:07.94 delay 2 seconds @00:42:09.97 AS.reconfigure ASconfig @00:42:10.02 AS.seek 0.5ml @00:42:20.33 delay 1 second @00:42:21.35 AFV.select :holdingCoil @00:42:21.45 ASV.dial :waste1 @00:42:21.85 ATV.dial :air3 @00:42:23.14 AS.reconfigure ASslow1 @00:42:23.20 AS.seek 0.51ml @00:42:25.48 delay 2 seconds @00:42:27.50 ATV.dial :bleach @00:42:28.11 AS.seek 0.81ml @00:43:28.74 delay 3 seconds @00:43:31.76 ATV.dial :air3 @00:43:32.32 delay 3 seconds @00:43:35.39 AS.seek 0.835ml @00:43:40.72 delay 3 seconds @00:43:43.74 ATV.dial :bleach @00:43:44.36 AS.seek 0.82ml @00:43:47.66 delay 3 seconds @00:43:50.68 ARV.dial :waste @00:43:51.32 delay 3 seconds @00:43:54.34 ATV.dial :ARV @00:43:55.25 AS.reconfigure ASconfig @00:43:55.31 AS.seek 0.47ml @00:44:15.71 AFV.select :carrierSolution @00:44:15.82 delay 2 seconds @00:44:17.85 AS.seek 1ml @00:44:48.63 delay 1 second @00:44:49.65 AFV.select :holdingCoil @00:44:49.75 delay 1 second @00:44:50.78 AS.reconfigure ASfast @00:44:50.84 AS.seek 0ml @00:45:18.52 AFV.select :carrierSolution @00:45:18.63 delay 2 seconds @00:45:20.66 AS.reconfigure ASconfig @00:45:20.72 AS.seek 1ml @00:46:18.47 delay 1 second @00:46:19.49 AFV.select :holdingCoil @00:46:19.59 delay 1 second @00:46:20.62 AS.reconfigure ASfast @00:46:20.67 AS.seek 0ml @00:46:48.19 AFV.select :carrierSolution @00:46:48.30 delay 2 seconds @00:46:50.33 AS.reconfigure ASconfig @00:46:50.38 AS.seek 1ml @00:47:48.13 delay 1 second @00:47:49.15 AFV.select :holdingCoil @00:47:49.25 delay 1 second @00:47:50.28 AS.reconfigure ASfast @00:47:50.33 AS.seek 0ml @00:48:17.96 ARV.dial between carrier and air1 @00:48:18.47 ATV.dial :air3 @00:48:19.78 AS.reconfigure ASslow1 @00:48:19.84 AS.seek 0.01ml @00:48:22.16 delay 2 seconds @00:48:24.18 ATV.dial :bleach @00:48:24.82 AS.seek 0.81ml @00:51:05.96 delay 3 seconds @00:51:08.98 ATV.dial :air3 @00:51:09.54 delay 3 seconds @00:51:12.61 AS.seek 0.835ml @00:51:17.85 delay 3 seconds @00:51:20.87 ATV.dial :bleach @00:51:21.49 AS.seek 0.82ml @00:51:24.79 delay 3 seconds @00:51:27.81 ATV.dial PCR @00:51:28.23 AS.reconfigure ASconfig @00:51:28.29 AS.seek :empty @00:52:15.77 PCR.program :bleachProfile @00:52:15.82 PCR program should complete in < 2:45 @00:52:15.83 PCR.run 10:00 @00:55:17.67 PCR.off @00:55:17.71 AFV.select :carrierSolution @00:55:17.82 delay 2 seconds @00:55:19.85 AS.seek 1ml @00:56:17.63 delay 1 second @00:56:18.65 AFV.select :holdingCoil @00:56:18.75 delay 1 second @00:56:19.78 AS.reconfigure ASfast @00:56:19.83 AS.seek 0ml @00:56:47.53 delay 3 seconds @00:56:50.88 AFV.select :carrierSolution @00:56:51.04 delay 2 seconds @00:56:53.07 AS.reconfigure ASconfig @00:56:53.14 AS.seek 1ml @00:57:50.92 delay 1 second @00:57:51.94 AFV.select :holdingCoil @00:57:52.04 delay 1 second @00:57:53.07 AS.reconfigure ASfast @00:57:53.13 AS.seek 0ml @00:58:20.57 delay 3 seconds @00:58:23.59 AFV.select :carrierSolution @00:58:23.70 delay 2 seconds @00:58:25.74 AS.reconfigure ASconfig @00:58:25.80 AS.seek 1ml @00:59:23.56 delay 1 second @00:59:24.58 AFV.select :holdingCoil @00:59:24.68 delay 1 second @00:59:25.71 AS.reconfigure ASfast @00:59:25.76 AS.seek 0ml @00:59:53.26 delay 3 seconds @00:59:56.28 AFV.select :carrierSolution @00:59:56.38 delay 2 seconds @00:59:58.41 AS.reconfigure ASconfig @00:59:58.47 AS.seek 1ml @01:00:56.23 delay 1 second @01:00:57.25 AFV.select :holdingCoil @01:00:57.35 delay 1 second @01:00:58.38 AS.reconfigure ASfast @01:00:58.44 AS.seek 0ml @01:01:25.79 delay 3 seconds @01:01:28.81 AFV.select :carrierSolution @01:01:28.91 delay 2 seconds @01:01:30.94 AS.reconfigure ASconfig @01:01:31.00 AS.seek 1ml @01:02:28.76 delay 1 second @01:02:29.78 AFV.select :holdingCoil @01:02:29.88 delay 1 second @01:02:30.91 AS.reconfigure ASnorm @01:02:30.96 AS.seek 0ml @01:03:21.68
AFV.select :carrierSolution @01:03:21.79 delay 2 seconds @01:03:23.82 AS.reconfigure ASconfig @01:03:23.87 AS.seek 0.5ml @01:03:52.92 delay 1 second @01:03:53.94 AFV.select :holdingCoil @01:03:54.04 ATV.dial :air1 @01:03:55.54 AS.reconfigure ASslow1 @01:03:55.60 AS.seek 0.52ml @01:03:59.89 delay 5 seconds @01:04:04.91 ATV.dial :extSrc @01:04:06.73 AS.reconfigure ASslow2 @01:04:06.79 AS.seek 0.545ml @01:04:32.14 delay 10 seconds @01:04:42.16 ATV.dial :air1 @01:04:43.87 AS.reconfigure ASslow1 @01:04:43.93 AS.seek 0.575ml @01:04:50.26 ATV.dial :waste @01:04:50.83 AS.reconfigure ASconfig @01:04:50.89 AS.seek :empty @01:05:24.45 Setting up PCR for Rp -- writing /var/log/esp/Rp18jul1701h.pcr @01:05:24.48 Building PCR Slug from extSrc with MM2 mix, PP3 primer @01:05:24.50 Priming with carrier @01:05:24.52 ATV.dial ARV @01:05:26.03 ARV.dial :carrier @01:05:26.39 AS.reconfigure ASnorm @01:05:26.45 AS.seek 0.499ml @01:05:52.03 AFV.select :carrierSolution @01:05:52.14 delay 2 seconds @01:05:54.17 AS.reconfigure ASconfig @01:05:54.23 AS.seek 1ml @01:06:23.34 delay 1 second @01:06:24.36 AFV.select :holdingCoil @01:06:24.46 delay 1 second @01:06:25.48 ATV.dial :waste @01:06:27.15 AS.reconfigure ASnorm @01:06:27.21 AS.seek :empty @01:07:18.00 AFV.select :carrierSolution @01:07:18.11 delay 2 seconds @01:07:20.14 AS.reconfigure ASconfig @01:07:20.19 AS.seek 0.5ml @01:07:49.30 delay 1 second @01:07:50.32 AFV.select :holdingCoil @01:07:50.42 Building Slug @01:07:50.43 ATV.dial :ARV @01:07:52.19 delay 2 seconds @01:07:54.21 ARV.dial :air1 @01:07:54.62 delay 2 seconds @01:07:56.69 AS.reconfigure ASslow2 @01:07:56.75 AS.seek 0.506ml @01:08:03.01 delay 2 seconds @01:08:05.03 ARV.dial :MM2 @01:08:06.56 delay 2 seconds @01:08:08.58 Adding MM2 @01:08:08.66 AS.seek 0.524ml @01:08:27.00 delay 25 seconds @01:08:52.02 ARV.dial :PP3 @01:08:52.42 Adding PP3 @01:08:52.43 delay 2 seconds @01:08:54.51 AS.seek 0.53ml @01:09:01.11 delay 10 seconds @01:09:11.14 ARV.dial :air2 @01:09:11.55 adding ARV air @01:09:11.56 delay 2 seconds @01:09:13.64 AS.seek 0.536ml @01:09:20.05 delay 5 seconds @01:09:25.07 ARV.dial :carrier @01:09:26.41 delay 7 seconds @01:09:33.49 AS.seek 0.594ml @01:10:31.98 delay 5 seconds @01:10:37.00 ATV.dial :extSrc @01:10:37.94 delay 2 seconds @01:10:40.01 AS.seek 0.6ml @01:10:46.31 delay 4 seconds @01:10:50.33 ATV.dial ARV @01:10:50.89 delay 2 seconds @01:10:52.91 ARV.dial :carrier @01:10:52.92 delay 2 seconds @01:10:55.01 AS.seek 0.65ml @01:11:45.45 delay 4 seconds @01:11:49.47 ATV.dial PCR @01:11:50.34 ASV.dial :waste1 @01:11:50.35 ARV.dial :air1 @01:11:50.78 Positioning Slug by 0.324ml @01:11:50.88 AS.seek 0.326ml @01:17:17.00 delay 5 seconds @01:17:22.02 ATV.dial ARV @01:17:22.80 ASV.dial :PCRlock @01:17:23.21 delay 5 seconds @01:17:28.24 Running Rp Profile60C300 thermocycling @01:17:28.25 PCR.program Profile60C300 @01:17:28.40 Profile60C300 should complete in < 1:45:21 @01:17:28.41 PCR.run 2:16:41.25 @02:38:11.25 PCR.off @02:38:12.76 PCR for Rp completed -- closed /var/log/esp/Rp18jul1701h.pcr @02:38:12.83 Cleaning PCR @02:38:12.84 AFV.select :carrierSolution @02:38:12.96 delay 2 seconds @02:38:14.99 AS.reconfigure ASconfig @02:38:15.04 AS.seek 0.5ml @02:38:25.35 delay 1 second @02:38:26.37 AFV.select :holdingCoil @02:38:26.47 ASV.dial :waste1 @02:38:26.86 ATV.dial :air3 @02:38:28.14 AS.reconfigure ASslow1 @02:38:28.20 AS.seek 0.51ml @02:38:30.47 delay 2 seconds @02:38:32.49 ATV.dial :bleach @02:38:33.11 AS.seek 0.81ml @02:39:33.71 delay 3 seconds @02:39:36.73 ATV.dial :air3 @02:39:37.29 delay 3 seconds @02:39:40.36 AS.seek 0.835ml @02:39:45.63 delay 3 seconds @02:39:49.02 ATV.dial :bleach @02:39:49.67 AS.seek 0.82ml @02:39:52.97 delay 3 seconds @02:39:55.99 ARV.dial :waste @02:39:56.63 delay 3 seconds @02:39:59.65 ATV.dial :ARV @02:40:00.66 AS.reconfigure ASconfig @02:40:00.72 AS.seek 0.47ml @02:40:21.13 AFV.select :carrierSolution @02:40:21.24 delay 2 seconds @02:40:23.27 AS.seek 1ml @02:40:54.02 delay 1 second @02:40:55.04 AFV.select :holdingCoil @02:40:55.14 delay 1 second @02:40:56.17 AS.reconfigure ASfast @02:40:56.22 AS.seek 0ml @02:41:24.15 AFV.select :carrierSolution @02:41:24.26 delay 2 seconds @02:41:26.29 AS.reconfigure ASconfig @02:41:26.34 AS.seek 1ml @02:42:24.10 delay 1 second @02:42:25.12 AFV.select :holdingCoil @02:42:25.22 delay 1 second @02:42:26.25 AS.reconfigure ASfast @02:42:26.31 AS.seek 0ml @02:42:53.94 AFV.select :carrierSolution @02:42:54.05 delay 2 seconds @02:42:56.08 AS.reconfigure ASconfig @02:42:56.13 AS.seek 1ml @02:43:53.90 delay 1 second @02:43:54.92 AFV.select :holdingCoil @02:43:55.02 delay 1 second @02:43:56.05 AS.reconfigure ASfast @02:43:56.10 AS.seek 0ml @02:44:23.68 ARV.dial between carrier and air1 @02:44:24.19 ATV.dial :air3 @02:44:25.49 AS.reconfigure ASslow1 @02:44:25.55 AS.seek 0.01ml @02:44:27.85 delay 2 seconds @02:44:29.87 ATV.dial :bleach @02:44:30.50 AS.seek 0.81ml @02:47:11.62 delay 3 seconds @02:47:14.64 ATV.dial :air3 @02:47:15.20 delay 3 seconds @02:47:18.27 AS.seek 0.835ml @02:47:23.59 delay 3 seconds @02:47:26.61 ATV.dial :bleach @02:47:27.24 AS.seek 0.82ml @02:47:30.59 delay 3 seconds @02:47:33.61 ATV.dial PCR @02:47:34.02 AS.reconfigure ASconfig @02:47:34.08 AS.seek :empty @02:48:21.57 PCR.program :bleachProfile @02:48:21.63 PCR program should complete in < 2:45 @02:48:21.64 PCR.run 10:00 @02:49:25.01 Session Ended @02:51:26.51 PCR.off @02:51:26.54 AFV.select :carrierSolution @02:51:26.64 delay 2 seconds @02:51:28.67 AS.seek 1ml @02:52:26.45 delay 1 second @02:52:27.47 AFV.select :holdingCoil @02:52:27.57 delay 1 second @02:52:28.60 AS.reconfigure ASfast @02:52:28.64 AS.seek 0ml @02:52:56.39 delay 3 seconds @02:52:59.41 AFV.select :carrierSolution @02:52:59.51 delay 2 seconds @02:53:01.54 AS.reconfigure ASconfig @02:53:01.58 AS.seek 1ml @02:53:59.34 delay 1 second @02:54:00.36 AFV.select :holdingCoil @02:54:00.46 delay 1 second @02:54:01.49 AS.reconfigure ASfast @02:54:01.53 AS.seek 0ml @02:54:29.15 delay 3 seconds @02:54:32.17 AFV.select :carrierSolution @02:54:32.27 delay 2 seconds @02:54:34.30 AS.reconfigure ASconfig @02:54:34.34 AS.seek 1ml @02:55:32.09 delay 1 second @02:55:33.11 AFV.select :holdingCoil @02:55:33.21 delay 1 second @02:55:34.24 AS.reconfigure ASfast @02:55:34.28 AS.seek 0ml @02:56:01.84 delay 3 seconds @02:56:04.86 AFV.select :carrierSolution @02:56:04.96 delay 2 seconds @02:56:06.99 AS.reconfigure ASconfig @02:56:07.06 AS.seek 1ml @02:57:04.84 delay 1 second @02:57:05.86 AFV.select :holdingCoil @02:57:05.95 delay 1 second @02:57:06.98 AS.reconfigure ASfast @02:57:07.02 AS.seek 0ml @02:57:34.62 delay 3 seconds @02:57:37.64 AFV.select :carrierSolution @02:57:37.74 delay 2 seconds @02:57:39.77 AS.reconfigure ASconfig @02:57:39.82 AS.seek 1ml @02:58:37.58 delay 1 second @02:58:38.60 AFV.select :holdingCoil @02:58:38.70 delay 1 second @02:58:39.73 AS.reconfigure ASnorm @02:58:40.16 AS.seek 0ml @02:59:30.85
AFV.select :carrierSolution @02:59:30.95 delay 2 seconds @02:59:32.98 AS.reconfigure ASconfig @02:59:33.02 AS.seek 0.5ml @03:00:02.09 delay 1 second @03:00:03.11 AFV.select :holdingCoil @03:00:03.21 ATV.dial :air1 @03:00:04.71 AS.reconfigure ASslow2 @03:00:04.75 AS.seek 0.506ml @03:00:11.00 ATV.dial :extSrc @03:00:12.68 AS.seek 0.512ml @03:00:18.92 delay 5 seconds @03:00:23.94 ATV.dial :air1 @03:00:25.65 AS.reconfigure ASslow1 @03:00:25.69 AS.seek 0.542ml @03:00:32.06 ATV.dial :waste @03:00:32.61 AS.reconfigure ASconfig @03:00:32.66 AS.seek :empty @03:01:04.28 Setting up PCR for Rp -- writing /var/log/esp/Rp18jul1703h.pcr @03:01:04.31 Building PCR Slug from extSrc with MM2 mix, PP3 primer @03:01:04.32 Priming with carrier @03:01:04.33 ATV.dial ARV @03:01:05.90 ARV.dial :carrier @03:01:06.25 AS.reconfigure ASnorm @03:01:06.29 AS.seek 0.499ml @03:01:31.84 AFV.select :carrierSolution @03:01:31.94 delay 2 seconds @03:01:33.97 AS.reconfigure ASconfig @03:01:34.01 AS.seek 1ml @03:02:03.08 delay 1 second @03:02:04.10 AFV.select :holdingCoil @03:02:04.20 delay 1 second @03:02:05.22 ATV.dial :waste @03:02:06.87 AS.reconfigure ASnorm @03:02:06.92 AS.seek :empty @03:02:57.66 AFV.select :carrierSolution @03:02:57.76 delay 2 seconds @03:02:59.79 AS.reconfigure ASconfig @03:02:59.83 AS.seek 0.5ml @03:03:28.94 delay 1 second @03:03:29.96 AFV.select :holdingCoil @03:03:30.06 Building Slug @03:03:30.07 ATV.dial :ARV @03:03:31.81 delay 2 seconds @03:03:33.83 ARV.dial :air1 @03:03:34.23 delay 2 seconds @03:03:36.30 AS.reconfigure ASslow2 @03:03:36.35 AS.seek 0.506ml @03:03:42.61 delay 2 seconds @03:03:44.63 ARV.dial :MM2 @03:03:46.16 delay 2 seconds @03:03:48.18 Adding MM2 @03:03:48.26 AS.seek 0.524ml @03:04:06.45 delay 25 seconds @03:04:31.47 ARV.dial :PP3 @03:04:31.87 Adding PP3 @03:04:31.88 delay 2 seconds @03:04:33.95 AS.seek 0.53ml @03:04:40.17 delay 10 seconds @03:04:50.20 ARV.dial :air2 @03:04:50.59 adding ARV air @03:04:50.60 delay 2 seconds @03:04:52.67 AS.seek 0.536ml @03:04:58.83 delay 5 seconds @03:05:03.85 ARV.dial :carrier @03:05:05.17 delay 7 seconds @03:05:12.25 AS.seek 0.594ml @03:06:10.75 delay 5 seconds @03:06:15.77 ATV.dial :extSrc @03:06:16.31 delay 2 seconds @03:06:18.38 AS.seek 0.6ml @03:06:24.61 delay 4 seconds @03:06:28.63 ATV.dial ARV @03:06:29.17 delay 2 seconds @03:06:31.19 ARV.dial :carrier @03:06:31.20 delay 2 seconds @03:06:33.27 AS.seek 0.65ml @03:07:23.91 delay 4 seconds @03:07:27.93 ATV.dial PCR @03:07:28.69 ASV.dial :waste1 @03:07:28.70 ARV.dial :air1 @03:07:29.14 Positioning Slug by 0.324ml @03:07:29.21 AS.seek 0.326ml @03:12:55.21 delay 5 seconds @03:13:00.23 ATV.dial ARV @03:13:01.00 ASV.dial :PCRlock @03:13:01.39 delay 5 seconds @03:13:06.41 Running Rp Profile60C300 thermocycling @03:13:06.43 PCR.program Profile60C300 @03:13:06.55 Profile60C300 should complete in < 1:45:21 @03:13:06.56 PCR.run 2:16:41.25 @04:33:47.43 PCR.off @04:33:48.93 PCR for Rp completed -- closed /var/log/esp/Rp18jul1703h.pcr @04:33:48.99 Cleaning PCR @04:33:49.00 AFV.select :carrierSolution @04:33:49.10 delay 2 seconds @04:33:51.13 AS.reconfigure ASconfig @04:33:51.17 AS.seek 0.5ml @04:34:01.51 delay 1 second @04:34:02.53 AFV.select :holdingCoil @04:34:02.63 ASV.dial :waste1 @04:34:03.02 ATV.dial :air3 @04:34:04.30 AS.reconfigure ASslow1 @04:34:04.34 AS.seek 0.51ml @04:34:06.66 delay 2 seconds @04:34:08.68 ATV.dial :bleach @04:34:09.28 AS.seek 0.81ml @04:35:09.86 delay 3 seconds @04:35:12.88 ATV.dial :air3 @04:35:13.43 delay 3 seconds @04:35:16.50 AS.seek 0.835ml @04:35:21.83 delay 3 seconds @04:35:24.85 ATV.dial :bleach @04:35:25.46 AS.seek 0.82ml @04:35:28.76 delay 3 seconds @04:35:31.78 ARV.dial :waste @04:35:32.41 delay 3 seconds @04:35:35.43 ATV.dial :ARV @04:35:36.32 AS.reconfigure ASconfig @04:35:36.37 AS.seek 0.47ml @04:35:56.77 AFV.select :carrierSolution @04:35:56.87 delay 2 seconds @04:35:58.90 AS.seek 1ml @04:36:29.63 delay 1 second @04:36:30.65 AFV.select :holdingCoil @04:36:30.74 delay 1 second @04:36:31.77 AS.reconfigure ASfast @04:36:31.81 AS.seek 0ml @04:36:59.60 AFV.select :carrierSolution @04:36:59.70 delay 2 seconds @04:37:01.73 AS.reconfigure ASconfig @04:37:01.77 AS.seek 1ml @04:37:59.51 delay 1 second @04:38:00.53 AFV.select :holdingCoil @04:38:00.63 delay 1 second @04:38:01.66 AS.reconfigure ASfast @04:38:01.70 AS.seek 0ml @04:38:29.52 AFV.select :carrierSolution @04:38:29.62 delay 2 seconds @04:38:31.65 AS.reconfigure ASconfig @04:38:31.69 AS.seek 1ml @04:39:29.44 delay 1 second @04:39:30.46 AFV.select :holdingCoil @04:39:30.56 delay 1 second @04:39:31.59 AS.reconfigure ASfast @04:39:31.63 AS.seek 0ml @04:39:59.63 ARV.dial between carrier and air1 @04:40:00.13 ATV.dial :air3 @04:40:01.41 AS.reconfigure ASslow1 @04:40:01.45 AS.seek 0.01ml @04:40:03.77 delay 2 seconds @04:40:05.79 ATV.dial :bleach @04:40:06.40 AS.seek 0.81ml @04:42:47.54 delay 3 seconds @04:42:50.56 ATV.dial :air3 @04:42:51.10 delay 3 seconds @04:42:54.17 AS.seek 0.835ml @04:42:59.49 delay 3 seconds @04:43:02.51 ATV.dial :bleach @04:43:03.12 AS.seek 0.82ml @04:43:06.41 delay 3 seconds @04:43:09.43 ATV.dial PCR @04:43:09.84 AS.reconfigure ASconfig @04:43:09.89 AS.seek :empty @04:43:57.34 PCR.program :bleachProfile @04:43:57.39 PCR program should complete in < 2:45 @04:43:57.40 PCR.run 10:00 @04:46:59.22 PCR.off @04:46:59.25 AFV.select :carrierSolution @04:46:59.35 delay 2 seconds @04:47:01.38 AS.seek 1ml @04:47:59.26 delay 1 second @04:48:00.28 AFV.select :holdingCoil @04:48:00.38 delay 1 second @04:48:01.41 AS.reconfigure ASfast @04:48:01.45 AS.seek 0ml @04:48:28.98 delay 3 seconds @04:48:32.00 AFV.select :carrierSolution @04:48:32.10 delay 2 seconds @04:48:34.13 AS.reconfigure ASconfig @04:48:34.17 AS.seek 1ml @04:49:31.93 delay 1 second @04:49:32.95 AFV.select :holdingCoil @04:49:33.05 delay 1 second @04:49:34.08 AS.reconfigure ASfast @04:49:34.12 AS.seek 0ml @04:50:01.59 delay 3 seconds @04:50:04.61 AFV.select :carrierSolution @04:50:04.71 delay 2 seconds @04:50:06.74 AS.reconfigure ASconfig @04:50:06.81 AS.seek 1ml @04:51:04.56 delay 1 second @04:51:05.58 AFV.select :holdingCoil @04:51:05.68 delay 1 second @04:51:06.71 AS.reconfigure ASfast @04:51:06.75 AS.seek 0ml @04:51:34.45 delay 3 seconds @04:51:37.47 AFV.select :carrierSolution @04:51:37.57 delay 2 seconds @04:51:39.60 AS.reconfigure ASconfig @04:51:39.64 AS.seek 1ml @04:52:37.39 delay 1 second @04:52:38.41 AFV.select :holdingCoil @04:52:38.50 delay 1 second @04:52:39.53 AS.reconfigure ASfast @04:52:39.57 AS.seek 0ml @04:53:07.12 delay 3 seconds @04:53:10.14 AFV.select :carrierSolution @04:53:10.24 delay 2 seconds @04:53:12.27 AS.reconfigure ASconfig @04:53:12.32 AS.seek 1ml @04:54:10.11 delay 1 second @04:54:11.13 AFV.select :holdingCoil @04:54:11.23 delay 1 second @04:54:12.26 AS.reconfigure ASnorm @04:54:12.30 AS.seek 0ml @04:55:03.01
AFV.select :carrierSolution @04:55:03.11 delay 2 seconds @04:55:05.14 AS.reconfigure ASconfig @04:55:05.18 AS.seek 0.5ml @04:55:34.23 delay 1 second @04:55:35.25 AFV.select :holdingCoil @04:55:35.35 ATV.dial :air1 @04:55:36.85 AS.reconfigure ASslow1 @04:55:36.90 AS.seek 0.52ml @04:55:41.19 delay 5 seconds @04:55:46.21 ATV.dial :extSrc @04:55:48.01 AS.reconfigure ASslow2 @04:55:48.05 AS.seek 0.538ml @04:56:06.45 delay 10 seconds @04:56:16.47 ATV.dial :air1 @04:56:18.27 AS.reconfigure ASslow1 @04:56:18.32 AS.seek 0.568ml @04:56:24.62 ATV.dial :waste @04:56:25.16 AS.reconfigure ASconfig @04:56:25.20 AS.seek :empty @04:56:58.21 deconEluateRecoveryLine extSrc ATV.dial :waste @04:56:58.23 AS.reconfigure ASnorm @04:56:58.27 AS.seek 0ml @04:56:58.67 ATV.dial :air3 @04:56:59.34 AS.reconfigure ASslow1 @04:56:59.38 AS.seek 0.01ml @04:57:01.66 delay 2 seconds @04:57:03.68 ATV.dial :bleach @04:57:04.14 AS.seek 0.31ml @04:58:05.19 delay 3 seconds @04:58:08.21 ATV.dial :air3 @04:58:08.76 delay 3 seconds @04:58:11.83 AS.seek 0.335ml @04:58:17.13 delay 3 seconds @04:58:20.15 ATV.dial :bleach @04:58:20.76 AS.seek 0.32ml @04:58:24.00 delay 3 seconds @04:58:27.02 ATV.dial :extSrc @04:58:27.63 AS.seek 0ml @04:59:32.25 AFV.select :carrierSolution @04:59:32.35 delay 2 seconds @04:59:34.38 AS.reconfigure ASconfig @04:59:34.42 AS.seek 0.5ml @05:00:03.45 delay 1 second @05:00:04.47 AFV.select :holdingCoil @05:00:04.57 ATV.dial :extSrc @05:00:04.59 AS.reconfigure ASslow1 @05:00:04.63 AS.seek 0ml @05:01:45.50 AFV.select :carrierSolution @05:01:45.60 delay 2 seconds @05:01:47.63 AS.reconfigure ASconfig @05:01:47.67 AS.seek 0.5ml @05:02:16.70 delay 1 second @05:02:17.72 AFV.select :holdingCoil @05:02:17.82 ATV.dial :extSrc @05:02:17.84 AS.reconfigure ASslow1 @05:02:17.88 AS.seek 0ml @05:03:58.70 AFV.select :carrierSolution @05:03:58.80 delay 2 seconds @05:04:00.83 AS.reconfigure ASconfig @05:04:00.87 AS.seek 0.5ml @05:04:29.91 delay 1 second @05:04:30.93 AFV.select :holdingCoil @05:04:31.03 ATV.dial :extSrc @05:04:31.05 AS.reconfigure ASslow1 @05:04:31.09 AS.seek 0ml @05:06:11.92 AFV.select :carrierSolution @05:06:12.02 delay 2 seconds @05:06:14.05 AS.reconfigure ASconfig @05:06:14.09 AS.seek 0.5ml @05:06:43.13 delay 1 second @05:06:44.15 AFV.select :holdingCoil @05:06:44.25 ATV.dial :extSrc @05:06:44.27 AS.reconfigure ASslow1 @05:06:44.31 AS.seek 0ml @05:08:25.13 ATV.dial :air2 @05:08:26.45 AS.reconfigure ASslow0 @05:08:26.49 AS.seek 0.5ml @05:09:17.13 ATV.dial :extSrc @05:09:18.60 AS.seek 0.02ml @05:10:07.16 delay 1 second @05:10:08.18 ATV.dial :waste @05:10:09.46 AS.reconfigure ASconfig @05:10:09.50 AS.seek :empty @05:10:10.99 deconEluateRecoveryLine extSrc @05:10:11.00 ATV.dial :waste @05:10:11.01 AS.reconfigure ASnorm @05:10:11.05 AS.seek 0ml @05:10:11.46 ATV.dial :air3 @05:10:12.41 AS.reconfigure ASslow1 @05:10:12.45 AS.seek 0.01ml @05:10:14.64 delay 2 seconds @05:10:16.66 ATV.dial :bleach @05:10:17.11 AS.seek 0.31ml @05:11:17.74 delay 3 seconds @05:11:20.76 ATV.dial :air3 @05:11:21.29 delay 3 seconds @05:11:24.36 AS.seek 0.335ml @05:11:29.68 delay 3 seconds @05:11:32.70 ATV.dial :bleach @05:11:33.30 AS.seek 0.32ml @05:11:36.60 delay 3 seconds @05:11:39.62 ATV.dial :extSrc @05:11:40.23 AS.seek 0ml @05:12:44.79 AFV.select :carrierSolution @05:12:44.89 delay 2 seconds @05:12:46.92 AS.reconfigure ASconfig @05:12:46.96 AS.seek 0.5ml @05:13:16.02 delay 1 second @05:13:17.04 AFV.select :holdingCoil @05:13:17.14 ATV.dial :extSrc @05:13:17.16 AS.reconfigure ASslow1 @05:13:17.20 AS.seek 0ml @05:14:57.97 AFV.select :carrierSolution @05:14:58.07 delay 2 seconds @05:15:00.10 AS.reconfigure ASconfig @05:15:00.14 AS.seek 0.5ml @05:15:29.16 delay 1 second @05:15:30.18 AFV.select :holdingCoil @05:15:30.28 ATV.dial :extSrc @05:15:30.30 AS.reconfigure ASslow1 @05:15:30.34 AS.seek 0ml @05:17:11.18 AFV.select :carrierSolution @05:17:11.28 delay 2 seconds @05:17:13.31 AS.reconfigure ASconfig @05:17:13.35 AS.seek 0.5ml @05:17:42.38 delay 1 second @05:17:43.40 AFV.select :holdingCoil @05:17:43.50 ATV.dial :extSrc @05:17:43.52 AS.reconfigure ASslow1 @05:17:43.56 AS.seek 0ml @05:19:24.43 AFV.select :carrierSolution @05:19:24.53 delay 2 seconds @05:19:26.56 AS.reconfigure ASconfig @05:19:26.60 AS.seek 0.5ml @05:19:55.63 delay 1 second @05:19:56.65 AFV.select :holdingCoil @05:19:56.75 ATV.dial :extSrc @05:19:56.77 AS.reconfigure ASslow1 @05:19:56.81 AS.seek 0ml @05:21:37.63 ATV.dial :air2 @05:21:38.98 AS.reconfigure ASslow0 @05:21:39.02 AS.seek 0.5ml @05:22:29.65 ATV.dial :extSrc @05:22:31.16 AS.seek 0.02ml @05:23:19.79 delay 1 second @05:23:20.81 ATV.dial :waste @05:23:22.09 AS.reconfigure ASconfig @05:23:22.13 AS.seek :empty @05:23:23.62 deconEluateRecoveryLine extSrc @05:23:23.63 ATV.dial :waste @05:23:23.64 AS.reconfigure ASnorm @05:23:23.69 AS.seek 0ml @05:23:24.01 ATV.dial :air3 @05:23:24.94 AS.reconfigure ASslow1 @05:23:24.98 AS.seek 0.01ml @05:23:27.24 delay 2 seconds @05:23:29.26 ATV.dial :bleach @05:23:29.73 AS.seek 0.31ml @05:24:30.31 delay 3 seconds @05:24:33.33 ATV.dial :air3 @05:24:33.87 delay 3 seconds @05:24:36.94 AS.seek 0.335ml @05:24:42.25 delay 3 seconds @05:24:45.27 ATV.dial :bleach @05:24:45.88 AS.seek 0.32ml @05:24:49.18 delay 3 seconds @05:24:52.20 ATV.dial :extSrc @05:24:52.93 AS.seek 0ml @05:25:57.54 AFV.select :carrierSolution @05:25:57.64 delay 2 seconds @05:25:59.67 AS.reconfigure ASconfig @05:25:59.71 AS.seek 0.5ml @05:26:28.78 delay 1 second @05:26:29.80 AFV.select :holdingCoil @05:26:29.90 ATV.dial :extSrc @05:26:29.92 AS.reconfigure ASslow1 @05:26:29.96 AS.seek 0ml @05:28:10.79 AFV.select :carrierSolution @05:28:10.89 delay 2 seconds @05:28:12.92 AS.reconfigure ASconfig @05:28:12.96 AS.seek 0.5ml @05:28:42.00 delay 1 second @05:28:43.02 AFV.select :holdingCoil @05:28:43.12 ATV.dial :extSrc @05:28:43.14 AS.reconfigure ASslow1 @05:28:43.18 AS.seek 0ml @05:30:24.00 AFV.select :carrierSolution @05:30:24.10 delay 2 seconds @05:30:26.13 AS.reconfigure ASconfig @05:30:26.17 AS.seek 0.5ml @05:30:55.20 delay 1 second @05:30:56.22 AFV.select :holdingCoil @05:30:56.32 ATV.dial :extSrc @05:30:56.34 AS.reconfigure ASslow1 @05:30:56.38 AS.seek 0ml @05:32:37.20 AFV.select :carrierSolution @05:32:37.30 delay 2 seconds @05:32:39.33 AS.reconfigure ASconfig @05:32:39.37 AS.seek 0.5ml @05:33:08.40 delay 1 second @05:33:09.42 AFV.select :holdingCoil @05:33:09.52 ATV.dial :extSrc @05:33:09.54 AS.reconfigure ASslow1 @05:33:09.58 AS.seek 0ml @05:34:50.39 ATV.dial :air2 @05:34:51.73 AS.reconfigure ASslow0 @05:34:51.77 AS.seek 0.5ml @05:35:42.40 ATV.dial :extSrc @05:35:43.89 AS.seek 0.02ml @05:36:32.48 delay 1 second @05:36:33.50 ATV.dial :waste @05:36:34.78 AS.reconfigure ASconfig @05:36:34.82 AS.seek :empty @13:09:19.20CEST07-Aug-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @13:09:20.87 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @13:09:21.12 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @13:09:25.32 Polling Can every 10:00 @13:09:27.07 SPE.coast @13:09:27.11 SPE.configure SPEconfig @13:09:27.20 AS.reconfigure ASconfig @13:09:27.23 AS.hold @13:09:27.28 AS.configure ASconfig @13:09:27.37 SS.reconfigure SSconfig @13:09:27.40 SS.hold @13:09:27.46 SS.configure SSconfig @13:09:27.55 CC.reconfigure CCconfig @13:09:27.58 CC.hold @13:09:27.63 CC.configure CCconfig @13:09:27.73 CS.reconfigure CSconfig @13:09:27.76 CS.hold @13:09:27.81 CS.configure CSconfig @13:09:28.23 PC.reconfigure PCconfig @13:09:28.27 PC.hold @13:09:28.31 PC.configure PCconfig @13:09:28.39 PS.configure PSconfig @13:09:28.49 Forearm.reconfigure LooseForearm @13:09:28.53 Forearm.hold @13:09:28.57 Forearm.configure LooseForearm @13:09:28.65 Elbow.reconfigure LooseElbow @13:09:28.69 Elbow.hold @13:09:28.74 Elbow.configure LooseElbow @13:09:28.84 SC.reconfigure SCloose @13:09:28.88 SC.hold @13:09:28.92 SC.configure SCloose @13:09:29.00 SE.reconfigure SEconfig @13:09:29.04 SE.hold @13:09:29.10 SE.configure SEconfig @13:09:29.14 CH.coast @13:09:29.19 CH.configure CHconfig @13:09:29.21 PH.coast @13:09:29.26 PH.configure PHconfig @13:09:29.30 PCR.off @13:09:29.32 PCR.pidtemp= 50,0.1,0,300,25,25 @13:09:29.35 PCR.caltemp= 2.7073,0.0373,0 @13:09:29.38 All dwarf controllers running firmware version 3.75 @13:09:29.53 Cleanly exited at 13:00:56CEST07-Aug-18 @13:09:29.56 Loading /home/esp/esp2/mission/stdMER2017.rb mission @13:09:30.07 AFV.select :carrierSolution @13:09:30.17 delay 2 seconds @13:09:32.20 AS.seek 0.5ml @13:10:01.31 delay 1 second @13:10:02.33 AFV.select :holdingCoil @13:10:02.42 ATV.dial :extSrc @13:10:03.89 AS.reconfigure ASslow1 @13:10:03.94 AS.seek 0.53ml @13:10:10.24 delay 5 seconds @13:10:15.32 AS.reconfigure ASslow2 @13:10:15.36 AS.seek 0.54ml @13:10:25.63 delay 20 seconds @13:10:45.70 AS.reconfigure ASslow1 @13:10:45.74 AS.seek 0.57ml @13:10:52.03 delay 5 seconds @13:15:48.30CEST07-Aug-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @13:15:49.96 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @13:15:50.21 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @13:15:54.48 Polling Can every 10:00 @13:15:56.22 SPE.coast @13:15:56.27 SPE.configure SPEconfig @13:15:56.35 AS.reconfigure ASconfig @13:15:56.39 AS.hold @13:15:56.45 AS.configure ASconfig @13:15:56.53 SS.reconfigure SSconfig @13:15:56.57 SS.hold @13:15:56.61 SS.configure SSconfig @13:15:56.69 CC.reconfigure CCconfig @13:15:56.73 CC.hold @13:15:56.79 CC.configure CCconfig @13:15:56.86 CS.reconfigure CSconfig @13:15:56.90 CS.hold @13:15:56.97 CS.configure CSconfig @13:15:57.39 PC.reconfigure PCconfig @13:15:57.42 PC.hold @13:15:57.50 PC.configure PCconfig @13:15:57.57 PS.configure PSconfig @13:15:57.68 Forearm.reconfigure LooseForearm @13:15:57.72 Forearm.hold @13:15:57.78 Forearm.configure LooseForearm @13:15:57.86 Elbow.reconfigure LooseElbow @13:15:57.89 Elbow.hold @13:15:57.95 Elbow.configure LooseElbow @13:15:58.04 SC.reconfigure SCloose @13:15:58.08 SC.hold @13:15:58.13 SC.configure SCloose @13:15:58.21 SE.reconfigure SEconfig @13:15:58.25 SE.hold @13:15:58.31 SE.configure SEconfig @13:15:58.34 CH.coast @13:15:58.39 CH.configure CHconfig @13:15:58.42 PH.coast @13:15:58.46 PH.configure PHconfig @13:15:58.50 PCR.off @13:15:58.52 PCR.pidtemp= 50,0.1,0,300,25,25 @13:15:58.55 PCR.caltemp= 2.7073,0.0373,0 @13:15:58.58 All dwarf controllers running firmware version 3.75 @13:15:58.74 Cleanly exited at 13:15:14CEST07-Aug-18 @13:15:58.77 Loading /home/esp/esp2/mission/stdMER2017.rb mission @13:15:59.29 AFV.select :carrierSolution @13:15:59.39 delay 2 seconds @13:16:01.42 AS.seek 0.5ml @13:16:30.53 delay 1 second @13:16:31.55 AFV.select :holdingCoil @13:16:31.64 ATV.dial :extSrc @13:16:32.96 AS.reconfigure ASslow1 @13:16:33.00 AS.seek 0.53ml @13:16:39.32 delay 5 seconds @13:16:44.40 AS.reconfigure ASslow2 @13:16:44.44 AS.seek 0.54ml @13:16:54.71 delay 20 seconds @13:17:14.78 AS.reconfigure ASslow1 @13:17:14.82 AS.seek 0.57ml @13:17:21.13 delay 5 seconds @13:17:26.21 AS.reconfigure ASslow2 @13:17:26.25 AS.seek 0.58ml @13:17:36.68 delay 20 seconds @13:17:56.75 AS.reconfigure ASslow1 @13:17:56.79 AS.seek 0.61ml @13:18:03.12 delay 5 seconds @13:18:08.19 AS.reconfigure ASslow2 @13:18:08.24 AS.seek 0.62ml @13:18:18.60 delay 20 seconds @13:18:38.67 AS.reconfigure ASslow1 @13:18:38.71 AS.seek 0.65ml @13:18:45.03 delay 5 seconds @13:18:50.11 AS.reconfigure ASslow2 @13:18:50.15 AS.seek 0.66ml @13:19:00.34 delay 20 seconds @13:19:20.37 AS.reconfigure ASslow1 @13:19:20.41 AS.seek 0.705ml @13:19:29.74 delay 10 seconds @13:19:39.76 ATV.dial :air1 @13:19:41.46 AS.seek 0.725ml @13:19:45.76 delay 3 seconds @13:19:48.78 ATV.dial :waste @13:19:49.32 AS.reconfigure ASslow0 @13:19:49.36 AS.seek :empty @13:21:02.77 Setting up PCR for Ss -- writing /var/log/esp/Ss18aug0713h.pcr @13:21:02.80 Building PCR Slug from extSrc with MM1 mix, PP2 primer @13:21:02.81 Priming with carrier @13:21:02.82 ATV.dial ARV @13:21:04.29 ARV.dial :carrier @13:21:04.63 AS.reconfigure ASnorm @13:21:04.67 AS.seek 0.499ml @13:21:30.23 AFV.select :carrierSolution @13:21:30.33 delay 2 seconds @13:21:32.36 AS.reconfigure ASconfig @13:21:32.40 AS.seek 1ml @13:22:01.45 delay 1 second @13:22:02.47 AFV.select :holdingCoil @13:22:02.57 delay 1 second @13:22:03.59 ATV.dial :waste @13:22:05.24 AS.reconfigure ASnorm @13:22:05.28 AS.seek :empty @13:22:56.04 AFV.select :carrierSolution @13:22:56.14 delay 2 seconds @13:22:58.17 AS.reconfigure ASconfig @13:22:58.21 AS.seek 0.5ml @13:23:27.28 delay 1 second @13:23:28.30 AFV.select :holdingCoil @13:23:28.40 Building Slug @13:23:28.41 ATV.dial :ARV @13:23:30.04 delay 2 seconds @13:23:32.06 ARV.dial :air1 @13:23:32.45 delay 2 seconds @13:23:34.52 AS.reconfigure ASslow2 @13:23:34.56 AS.seek 0.506ml @13:23:40.92 delay 2 seconds @13:23:42.94 ARV.dial :MM1 @13:23:43.53 delay 2 seconds @13:23:45.55 Adding MM1 @13:23:45.61 AS.seek 0.524ml @13:24:03.93 delay 25 seconds @13:24:28.95 ARV.dial :PP2 @13:24:29.35 Adding PP2 @13:24:29.36 delay 2 seconds @13:24:31.43 AS.seek 0.53ml @13:24:37.67 delay 10 seconds @13:24:47.70 ARV.dial :air2 @13:24:48.07 adding ARV air @13:24:48.08 delay 2 seconds @13:24:50.15 AS.seek 0.536ml @13:24:56.40 delay 5 seconds @13:25:01.42 ARV.dial :carrier @13:25:02.70 delay 7 seconds @13:25:09.78 AS.seek 0.594ml @13:26:08.23 delay 5 seconds @13:26:13.25 ATV.dial :extSrc @13:26:13.81 delay 2 seconds @13:26:15.88 AS.seek 0.6ml @13:26:22.09 delay 4 seconds @13:26:26.11 ATV.dial ARV @13:26:26.65 delay 2 seconds @13:26:28.67 ARV.dial :carrier @13:26:28.68 delay 2 seconds @13:26:30.75 AS.seek 0.65ml @13:27:21.20 delay 4 seconds @13:27:25.22 ATV.dial PCR @13:27:25.98 ASV.dial :waste1 @13:27:26.06 ARV.dial :air1 @13:27:26.85 Positioning Slug by 0.324ml @13:27:26.92 AS.seek 0.326ml @13:32:53.00 delay 5 seconds @13:32:58.02 ATV.dial ARV @13:32:58.89 ASV.dial :PCRlock @13:32:59.28 delay 5 seconds @13:33:04.30 Running Ss Profile60C350 thermocycling @13:33:04.31 PCR.program Profile60C350 @13:33:04.44 Profile60C350 should complete in < 1:48:05 @13:33:04.45 PCR.run 2:20:06.25 @13:33:33.38 Session Begun @13:33:52.67 -> AS.stop @13:33:52.68 AS.stop @13:34:08.60 -> PCR.abort @13:34:08.61 PCR.abort @13:34:11.14 PCR.off @13:34:11.27 AnalyticalModule::PCRDataPoint::BadDataFormat in PCR -- Bad data format: "ERROR:No Data Stored" @13:34:11.33 BadNews.email "Bad data format: \"ERROR:No Data Stored\"",Subject: "AnalyticalModule::PCRDataPoint::BadDataFormat in PCR" @13:34:11.43 Thread::ChildDied in MFBPCR -- :PCR @13:34:11.49 Waiting to Resume from Checkpoint ":PCR" @13:34:18.49 BadNews.email ":PCR",Subject: "Thread::ChildDied in MFBPCR" @13:34:24.64 Session Begun @13:34:29.01 -> showlog 100 @13:36:10.98 -> ATV @13:36:25.53 -> ATV.waste @13:36:25.54 ATV.dial :waste @13:36:33.44 -> AS.empty @13:36:33.46 AS.reconfigure ASconfig @13:36:33.55 AS.seek :empty @13:38:05.12 -> ATV.ARV @13:38:05.13 ATV.dial ARV @13:38:44.39 -> ARV.air2 @13:38:44.40 ARV.dial :air2 @13:39:01.40 -> AS.pull 0.2, ASslow1 @13:39:01.49 AS.reconfigure ASslow1 @13:39:01.56 AS.seek 0.199ml @13:39:50.23 -> ATV @13:39:55.69 -> ARV.MM1 @13:39:55.70 ARV.dial :MM1 @13:40:11.28 -> AS.pull 0.006, ASslow1 @13:40:11.38 AS.seek 0.205ml @13:40:27.06 -> ARV.air2 @13:40:27.07 ARV.dial :air2 @13:40:32.85 -> AS.pull 0.03, ASslow1 @13:40:32.95 AS.seek 0.235ml @13:41:07.68 -> ARV.MM1 @13:41:07.69 ARV.dial :MM1 @13:41:12.84 -> AS.pull 0.006, ASslow2 @13:41:12.94 AS.reconfigure ASslow2 @13:41:13.01 AS.seek 0.241ml @13:41:24.40 -> ARV.air2 @13:41:24.42 ARV.dial :air2 @13:41:29.07 -> AS.pull 0.03, ASslow1 @13:41:29.23 AS.reconfigure ASslow1 @13:41:29.30 AS.seek 0.271ml @13:42:06.30 -> ARV.MM1 @13:42:06.31 ARV.dial :MM1 @13:42:10.23 -> AS.pull 0.006, ASslow2 @13:42:10.32 AS.reconfigure ASslow2 @13:42:10.39 AS.seek 0.277ml @13:42:28.11 -> ARV.air2 @13:42:28.12 ARV.dial :air2 @13:42:31.52 -> AS.pull 0.03, ASslow1 @13:42:31.62 AS.reconfigure ASslow1 @13:42:31.69 AS.seek 0.307ml @13:45:19.87 -> ARV.legend @13:45:21.28 -> ARV @13:46:04.16 -> ATV @13:46:10.84 -> AS.pull 0.05, ASslow1 @13:46:10.94 AS.seek 0.357ml @13:46:30.83 -> ARV.MM1 @13:46:30.84 ARV.dial :MM1 @13:46:46.47 -> AS.pull 0.006, ASslow2 @13:46:46.56 AS.reconfigure ASslow2 @13:46:46.63 AS.seek 0.363ml @13:47:18.08 -> ARV.MM1 @13:47:18.09 ARV.dial :MM1 @13:47:26.76 -> ARV.air2 @13:47:26.77 ARV.dial :air2 @13:47:38.18 -> AS.pull 0.05, ASslow1 @13:47:38.28 AS.reconfigure ASslow1 @13:47:38.73 AS.seek 0.413ml @13:47:47.32 AS.hold @13:47:47.58 UserAbort in mwj -- Analytical Syringe seek to 0.413ml Aborted! @13:48:16.32 -> ARV.MM1 @13:48:16.33 ARV.dial :MM1 @13:48:37.57 -> AS.pull 0.010, ASslow2 @13:48:37.67 AS.reconfigure ASslow2 @13:48:37.74 AS.seek 0.416ml @13:49:04.19 -> ARV.air2 @13:49:04.20 ARV.dial :air2 @13:49:18.43 -> AS.pull 0.05, ASslow2 @13:49:18.53 AS.seek 0.466ml @13:50:01.06 AS.hold @13:50:01.27 UserAbort in mwj -- Analytical Syringe seek to 0.466ml Aborted! @13:50:19.02 -> AS.pull 0.05, ASslow1 @13:50:19.11 AS.reconfigure ASslow1 @13:50:19.18 AS.seek 0.509ml @13:52:13.01 -> ARV.PP2 @13:52:13.02 ARV.dial :PP2 @13:52:19.46 -> AS.pull 0.006, ASslow2 @13:52:19.55 AS.reconfigure ASslow2 @13:52:19.62 AS.seek 0.515ml @13:52:37.57 -> ATV.air2 @13:52:37.58 ATV.dial :air2 @13:52:50.74 -> ATV.ARV @13:52:50.75 ATV.dial ARV @13:52:58.63 -> ARV.air2 @13:52:58.64 ARV.dial :air2 @13:53:25.22 -> AS.pull 0.05, ASslow1 @13:53:25.31 AS.reconfigure ASslow1 @13:53:25.38 AS.seek 0.565ml @13:53:51.74 -> ARV @13:53:56.28 -> ARV.legend @13:58:08.18 -> ARV.MM1 @13:58:08.19 ARV.dial :MM1 @13:58:28.77 -> ATV @13:58:34.93 -> AS.pull 0.01, ASslow2 @13:58:35.02 AS.reconfigure ASslow2 @13:58:35.09 AS.seek 0.575ml @14:01:54.04 -> ARV.air2 @14:01:54.05 ARV.dial :air2 @14:02:09.38 -> AS.pull 0.05, ASslow2 @14:02:09.48 AS.seek 0.625ml @14:03:43.05 -> pcrDecon @14:03:43.15 Cleaning PCR @14:03:43.16 AFV.select :carrierSolution @14:03:43.30 delay 2 seconds @14:03:45.33 AS.reconfigure ASconfig @14:03:45.40 AS.seek 0.5ml @14:03:52.88 delay 1 second @14:03:53.90 AFV.select :holdingCoil @14:03:54.02 ASV.dial :waste1 @14:03:54.44 ATV.dial :air3 @14:03:55.58 AS.reconfigure ASslow1 @14:03:55.65 AS.seek 0.51ml @14:03:58.01 delay 2 seconds @14:04:00.03 ATV.dial :bleach @14:04:00.66 AS.seek 0.81ml @14:05:01.28 delay 3 seconds @14:05:04.30 ATV.dial :air3 @14:05:04.86 delay 3 seconds @14:05:07.93 AS.seek 0.835ml @14:05:13.25 delay 3 seconds @14:05:16.27 ATV.dial :bleach @14:05:16.89 AS.seek 0.82ml @14:05:20.17 delay 3 seconds @14:05:23.19 ARV.dial :waste @14:05:24.71 delay 3 seconds @14:05:27.73 ATV.dial :ARV @14:05:28.64 AS.reconfigure ASconfig @14:05:28.71 AS.seek 0.47ml @14:05:49.14 AFV.select :carrierSolution @14:05:49.27 delay 2 seconds @14:05:51.30 AS.seek 1ml @14:06:22.07 delay 1 second @14:06:23.09 AFV.select :holdingCoil @14:06:23.21 delay 1 second @14:06:24.24 AS.reconfigure ASfast @14:06:24.30 AS.seek 0ml @14:06:52.02 AFV.select :carrierSolution @14:06:52.14 delay 2 seconds @14:06:54.17 AS.reconfigure ASconfig @14:06:54.23 AS.seek 1ml @14:07:52.02 delay 1 second @14:07:53.04 AFV.select :holdingCoil @14:07:53.16 delay 1 second @14:07:54.19 AS.reconfigure ASfast @14:07:54.25 AS.seek 0ml @14:08:21.88 AFV.select :carrierSolution @14:08:22.01 delay 2 seconds @14:08:24.03 AS.reconfigure ASconfig @14:08:24.10 AS.seek 1ml @14:09:21.88 delay 1 second @14:09:22.90 AFV.select :holdingCoil @14:09:23.01 delay 1 second @14:09:24.04 AS.reconfigure ASfast @14:09:24.10 AS.seek 0ml @14:09:51.74 ARV.dial between carrier and air1 @14:09:52.27 ATV.dial :air3 @14:09:53.58 AS.reconfigure ASslow1 @14:09:53.65 AS.seek 0.01ml @14:09:55.94 delay 2 seconds @14:09:57.96 ATV.dial :bleach @14:09:58.58 AS.seek 0.81ml @14:12:39.74 delay 3 seconds @14:12:42.76 ATV.dial :air3 @14:12:43.33 delay 3 seconds @14:12:46.40 AS.seek 0.835ml @14:12:51.71 delay 3 seconds @14:12:54.73 ATV.dial :bleach @14:12:55.36 AS.seek 0.82ml @14:12:58.63 delay 3 seconds @14:13:01.65 ATV.dial PCR @14:13:02.05 AS.reconfigure ASconfig @14:13:02.12 AS.seek :empty @14:13:49.63 PCR.program :bleachProfile @14:13:49.70 PCR program should complete in < 2:45 @14:13:49.71 PCR.run 10:00 @14:16:57.62 PCR.off @14:16:57.66 AFV.select :carrierSolution @14:16:57.79 delay 2 seconds @14:16:59.82 AS.seek 1ml @14:17:57.69 delay 1 second @14:17:58.71 AFV.select :holdingCoil @14:17:58.82 delay 1 second @14:17:59.85 AS.reconfigure ASfast @14:17:59.91 AS.seek 0ml @14:18:27.77 delay 3 seconds @14:18:30.79 AFV.select :carrierSolution @14:18:30.91 delay 2 seconds @14:18:32.94 AS.reconfigure ASconfig @14:18:33.01 AS.seek 1ml @14:19:30.79 delay 1 second @14:19:31.81 AFV.select :holdingCoil @14:19:31.93 delay 1 second @14:19:32.96 AS.reconfigure ASfast @14:19:33.02 AS.seek 0ml @14:20:00.68 delay 3 seconds @14:20:03.70 AFV.select :carrierSolution @14:20:03.82 delay 2 seconds @14:20:06.27 AS.reconfigure ASconfig @14:20:07.30 AS.seek 1ml @14:21:05.07 delay 1 second @14:21:06.09 AFV.select :holdingCoil @14:21:06.21 delay 1 second @14:21:07.24 AS.reconfigure ASfast @14:21:07.30 AS.seek 0ml @14:21:34.99 delay 3 seconds @14:21:38.01 AFV.select :carrierSolution @14:21:38.13 delay 2 seconds @14:21:40.16 AS.reconfigure ASconfig @14:21:40.23 AS.seek 1ml @14:22:37.99 delay 1 second @14:22:39.01 AFV.select :holdingCoil @14:22:39.14 delay 1 second @14:22:40.17 AS.reconfigure ASfast @14:22:40.23 AS.seek 0ml @14:23:07.94 delay 3 seconds @14:23:10.96 AFV.select :carrierSolution @14:23:11.07 delay 2 seconds @14:23:13.10 AS.reconfigure ASconfig @14:23:13.17 AS.seek 1ml @14:24:10.98 delay 1 second @14:24:12.00 AFV.select :holdingCoil @14:24:12.12 delay 1 second @14:24:13.15 AS.reconfigure ASnorm @14:24:13.21 AS.seek 0ml @14:41:50.60 -> exit @14:41:50.61 Session Ended @15:42:58.95CEST07-Aug-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @15:43:00.60 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @15:43:00.86 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @15:43:05.10 Polling Can every 10:00 @15:43:06.86 SPE.coast @15:43:06.91 SPE.configure SPEconfig @15:43:06.99 AS.reconfigure ASconfig @15:43:07.03 AS.hold @15:43:07.09 AS.configure ASconfig @15:43:07.18 SS.reconfigure SSconfig @15:43:07.21 SS.hold @15:43:07.26 SS.configure SSconfig @15:43:07.34 CC.reconfigure CCconfig @15:43:07.38 CC.hold @15:43:07.45 CC.configure CCconfig @15:43:07.53 CS.reconfigure CSconfig @15:43:07.57 CS.hold @15:43:07.64 CS.configure CSconfig @15:43:08.06 PC.reconfigure PCconfig @15:43:08.09 PC.hold @15:43:08.14 PC.configure PCconfig @15:43:08.22 PS.configure PSconfig @15:43:08.33 Forearm.reconfigure LooseForearm @15:43:08.37 Forearm.hold @15:43:08.42 Forearm.configure LooseForearm @15:43:08.50 Elbow.reconfigure LooseElbow @15:43:08.54 Elbow.hold @15:43:08.59 Elbow.configure LooseElbow @15:43:08.69 SC.reconfigure SCloose @15:43:08.73 SC.hold @15:43:08.78 SC.configure SCloose @15:43:08.86 SE.reconfigure SEconfig @15:43:08.90 SE.hold @15:43:08.95 SE.configure SEconfig @15:43:08.99 CH.coast @15:43:09.03 CH.configure CHconfig @15:43:09.06 PH.coast @15:43:09.11 PH.configure PHconfig @15:43:09.15 PCR.off @15:43:09.17 PCR.pidtemp= 50,0.1,0,300,25,25 @15:43:09.20 PCR.caltemp= 2.7073,0.0373,0 @15:43:09.23 All dwarf controllers running firmware version 3.75 @15:43:09.39 Cleanly exited at 15:42:20CEST07-Aug-18 @15:43:09.42 Loading /home/esp/esp2/mission/stdMER2017.rb mission @15:43:09.95 AFV.select :carrierSolution @15:43:10.05 delay 2 seconds @15:43:12.08 AS.seek 0.5ml @15:43:41.17 delay 1 second @15:43:42.19 AFV.select :holdingCoil @15:43:42.30 ATV.dial :extSrc @15:43:43.76 AS.reconfigure ASslow1 @15:43:43.80 AS.seek 0.53ml @15:43:50.17 delay 5 seconds @15:43:55.26 AS.reconfigure ASslow2 @15:43:55.30 AS.seek 0.54ml @15:44:05.82 delay 20 seconds @15:44:25.90 AS.reconfigure ASslow1 @15:44:25.94 AS.seek 0.57ml @15:44:32.29 delay 5 seconds @15:44:37.37 AS.reconfigure ASslow2 @15:44:37.41 AS.seek 0.58ml @15:44:47.63 delay 20 seconds @15:45:07.71 AS.reconfigure ASslow1 @15:45:07.75 AS.seek 0.61ml @15:45:14.02 delay 5 seconds @15:45:19.11 AS.reconfigure ASslow2 @15:45:19.15 AS.seek 0.62ml @15:45:29.37 delay 20 seconds @15:45:49.45 AS.reconfigure ASslow1 @15:45:49.49 AS.seek 0.65ml @15:45:55.76 delay 5 seconds @15:46:00.84 AS.reconfigure ASslow2 @15:46:00.89 AS.seek 0.66ml @15:46:11.26 delay 20 seconds @15:46:31.29 AS.reconfigure ASslow1 @15:46:31.34 AS.seek 0.705ml @15:46:40.71 delay 10 seconds @15:46:50.74 ATV.dial :air1 @15:46:52.43 AS.seek 0.725ml @15:46:56.74 delay 3 seconds @15:46:59.77 ATV.dial :waste @15:47:00.32 AS.reconfigure ASslow0 @15:47:00.36 AS.seek :empty @15:48:13.82 Setting up PCR for Ss -- writing /var/log/esp/Ss18aug0715h.pcr @15:48:13.84 Building PCR Slug from extSrc with MM1 mix, PP2 primer @15:48:13.86 Priming with carrier ATV.dial ARV @15:48:15.32 ARV.dial :carrier @15:48:15.66 AS.reconfigure ASnorm @15:48:15.71 AS.seek 0.499ml @15:48:41.21 AFV.select :carrierSolution @15:48:41.32 delay 2 seconds @15:48:43.34 AS.reconfigure ASconfig @15:48:43.39 AS.seek 1ml @15:49:12.45 delay 1 second @15:49:13.48 AFV.select :holdingCoil @15:49:13.58 delay 1 second @15:49:14.60 ATV.dial :waste @15:49:16.25 AS.reconfigure ASnorm @15:49:16.29 AS.seek :empty @15:50:07.03 AFV.select :carrierSolution @15:50:07.14 delay 2 seconds @15:50:09.16 AS.reconfigure ASconfig @15:50:09.21 AS.seek 0.5ml @15:50:38.31 delay 1 second @15:50:39.34 AFV.select :holdingCoil @15:50:39.44 Building Slug @15:50:39.45 ATV.dial :ARV @15:50:41.08 delay 2 seconds @15:50:43.10 ARV.dial :air1 @15:50:43.48 delay 2 seconds @15:50:45.56 AS.reconfigure ASslow2 @15:50:45.60 AS.seek 0.506ml @15:50:51.92 delay 2 seconds @15:50:53.95 ARV.dial :MM1 @15:50:54.54 delay 2 seconds @15:50:56.57 Adding MM1 @15:50:56.63 AS.seek 0.524ml @15:51:15.04 delay 25 seconds @15:51:40.07 ARV.dial :PP2 @15:51:40.46 Adding PP2 @15:51:40.47 delay 2 seconds @15:51:42.54 AS.seek 0.53ml @15:51:48.76 delay 10 seconds @15:51:58.80 ARV.dial :air2 @15:51:59.18 adding ARV air @15:51:59.19 delay 2 seconds @15:52:01.26 AS.seek 0.536ml @15:52:07.48 delay 5 seconds @15:52:12.51 ARV.dial :carrier @15:52:13.80 delay 7 seconds @15:52:20.88 AS.seek 0.594ml @15:53:19.42 delay 5 seconds @15:53:24.45 ATV.dial :extSrc @15:53:25.00 delay 2 seconds @15:53:27.08 AS.seek 0.6ml @15:53:33.32 delay 4 seconds @15:53:37.35 ATV.dial ARV @15:53:37.89 delay 2 seconds @15:53:39.92 ARV.dial :carrier @15:53:39.93 delay 2 seconds @15:53:42.00 AS.seek 0.65ml @15:54:32.71 delay 4 seconds @15:54:36.74 ATV.dial PCR @15:54:37.50 ASV.dial :waste1 @15:54:37.57 ARV.dial :air1 @15:54:38.38 Positioning Slug by 0.324ml @15:54:38.45 AS.seek 0.326ml @15:54:41.66 Session Begun @15:54:48.72 -> AS.stop @15:54:48.73 AS.stop @15:54:48.86 Slide::Error in MFBPCR -- Analytical Syringe aborted at 0.64ml @15:54:48.92 Waiting to Resume from Checkpoint "Analytical Syringe aborted at 0.64ml" @15:54:48.94 BadNews.email "Analytical Syringe aborted at 0.64ml",Subject: "Slide::Error in MFBPCR" @15:55:01.34 -> PCR.abort @15:55:01.35 PCR.abort @15:56:34.35 -> ATV.waste @15:56:34.36 ATV.dial :waste @15:56:42.06 -> AS.empty @15:56:42.08 AS.reconfigure ASconfig @15:56:42.15 AS.seek :empty @15:57:32.47 -> ATV.ARV @15:57:32.48 ATV.dial ARV @15:57:36.95 -> ARV @15:57:48.20 -> ARV.air2 @15:57:48.21 ARV.dial :air2 @15:58:04.34 -> AS.pull 0.20, ASslow1 @15:58:04.42 AS.reconfigure ASslow1 @15:58:04.49 AS.seek 0.199ml @15:59:07.84 -> ARV.MM1 @15:59:07.85 ARV.dial :MM1 @15:59:13.18 -> ATV @15:59:25.26 -> AS.pull 0.006, ASslow2 @15:59:25.34 AS.reconfigure ASslow2 @15:59:25.40 AS.seek 0.205ml @15:59:42.79 -> ARV.air2 @15:59:42.80 ARV.dial :air2 @15:59:54.03 -> AS.pull 0.030, ASslow1 @15:59:54.11 AS.reconfigure ASslow1 @15:59:54.17 AS.seek 0.235ml @16:02:11.86 -> ARV.MM1 @16:02:11.87 ARV.dial :MM1 @16:02:15.23 -> AS.pull 0.006, ASslow2 @16:02:15.31 AS.reconfigure ASslow2 @16:02:15.37 AS.seek 0.241ml @16:02:28.65 -> ARV.air1 @16:02:28.66 ARV.dial :air1 @16:02:37.38 -> AS.pull 0.020, ASslow1 @16:02:37.46 AS.reconfigure ASslow1 @16:02:37.52 AS.seek 0.261ml @16:14:13.10 -> ATV @16:14:28.57 -> ARV.carrierSolution @16:14:28.58 ARV.dial :carrierSolution @16:14:34.45 -> ATV.ARV @16:14:34.46 ATV.dial ARV @16:14:56.00 -> AS.pull 0.100, ASslow1 @16:14:56.08 AS.seek 0.361ml @16:16:04.66 Session Ended @16:16:20.00 Session Begun @16:16:24.66 -> ATV.waste @16:16:24.67 ATV.dial :waste @16:16:29.23 -> AS.empty @16:16:29.25 AS.reconfigure ASconfig @16:16:29.32 AS.seek :empty @16:17:19.95 -> shortmfb([Ss],[:extSrc]){}.finish @16:17:19.97 Waiting for previous MFBPCR to complete... @16:17:24.56 UserAbort in mwj -- @15:17:47.52CEST08-Aug-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @15:17:49.18 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @15:17:49.43 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @15:17:53.69 Polling Can every 10:00 @15:17:55.44 SPE.coast @15:17:55.49 SPE.configure SPEconfig @15:17:55.57 AS.reconfigure ASconfig @15:17:55.61 AS.hold @15:17:55.66 AS.configure ASconfig @15:17:55.74 SS.configure SSconfig @15:17:55.82 CC.configure CCconfig @15:17:55.90 CS.configure CSconfig @15:17:55.98 PC.configure PCconfig @15:17:56.06 PS.configure PSconfig @15:17:56.11 I2C::NodeOffline in MAIN -- manipulator Not Responding @15:17:56.12 MISSING Hand @15:17:56.15 I2C::NodeOffline in MAIN -- manipulator Not Responding MISSING Forearm @15:17:56.17 I2C::NodeOffline in MAIN -- manipulator Not Responding @15:17:56.18 MISSING Elbow @15:17:56.25 SC.configure SCloose @15:17:56.66 SE.configure SEconfig @15:17:56.70 CH.coast @15:17:56.74 CH.configure CHconfig @15:17:56.77 PH.coast @15:17:56.82 PH.configure PHconfig @15:17:56.85 PCR.off @15:17:56.88 PCR.pidtemp= 50,0.1,0,300,25,25 @15:17:56.90 PCR.caltemp= 2.7073,0.0373,0 @15:17:56.93 All dwarf controllers running firmware version 3.75 @15:17:57.04 Cleanly exited at 15:16:19CEST08-Aug-18 @15:17:57.07 Loading /home/esp/esp2/mission/stdMER2017.rb mission @15:17:57.17 Intake.select :close @15:17:57.21 I2C::NodeOffline in MAIN -- manipulator microcontroller never signed on while reading Forearm's status @15:17:57.22 Exhaust.select :close @15:17:57.24
Waiting to Resume from Checkpoint "Loading /home/esp/esp2/mission/stdMER2017.rb mission" @15:17:57.26 BadNews.email "manipulator microcontroller never signed on while reading Forearm's status",Subject: "I2C::NodeOffline in MAIN" @15:17:57.27 CTV.dial :air @15:18:42.33 I2C::Request::Timeout in rdySampler -- No Response to I2C::Shaft::RotateRequest[13:->27] @15:18:42.34 Retry #1 of 3 @15:18:42.38 I2C::UnexpectedReply in core -- I2C::Shaft::RotateReply[13:27->20]aborted at 4369 @15:19:27.37 I2C::Request::Timeout in rdySampler -- No Response to I2C::Shaft::RotateRequest[14:->27] @15:19:27.38 Retry #2 of 3 @15:19:27.42 I2C::UnexpectedReply in core -- I2C::Shaft::RotateReply[14:27->20]aborted at 4369 @15:20:12.41 I2C::Request::Timeout in rdySampler -- No Response to I2C::Shaft::RotateRequest[15:->27] @15:20:12.42 Retry #3 of 3 @15:20:12.46 I2C::UnexpectedReply in core -- I2C::Shaft::RotateReply[15:27->20]aborted at 4369 @15:20:57.45 CTV.stop @15:20:57.49 I2C::UnexpectedReply in core -- I2C::Shaft::RotateReply[16:27->20]aborted at 4369 @15:20:57.58 I2C::Request::Timeout in rdySampler -- No Response to I2C::Shaft::RotateRequest[16:->27] while rotating Collection Top Valve @15:20:57.59 Waiting to Resume from Checkpoint "No Response to I2C::Shaft::RotateRequest[16:->27] while rotating Collection Top Valve" @15:20:57.61 BadNews.email "No Response to I2C::Shaft::RotateRequest[16:->27] while rotating Collection Top Valve",Subject: "I2C::Request::Timeout in rdySampler" @15:22:08.81 Session Begun @15:22:12.86 -> showlog 100 @15:22:35.04 -> #hvad sker der? @15:39:36.20CEST08-Aug-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @15:39:38.14 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @15:39:38.39 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @15:39:42.64 Polling Can every 10:00 @15:39:44.39 SPE.coast @15:39:44.44 SPE.configure SPEconfig @15:39:44.52 AS.reconfigure ASconfig @15:39:44.56 AS.hold @15:39:44.62 AS.configure ASconfig @15:39:44.70 SS.reconfigure SSconfig @15:39:44.74 SS.hold @15:39:44.80 SS.configure SSconfig @15:39:44.88 CC.reconfigure CCconfig @15:39:44.92 CC.hold @15:39:44.96 CC.configure CCconfig @15:39:45.04 CS.reconfigure CSconfig @15:39:45.08 CS.hold @15:39:45.15 CS.configure CSconfig @15:39:45.57 PC.reconfigure PCconfig @15:39:45.60 PC.hold @15:39:45.67 PC.configure PCconfig @15:39:45.75 PS.configure PSconfig @15:39:45.85 Forearm.reconfigure LooseForearm @15:39:45.89 Forearm.hold @15:39:45.93 Forearm.configure LooseForearm @15:39:46.02 Elbow.reconfigure LooseElbow @15:39:46.06 Elbow.hold @15:39:46.10 Elbow.configure LooseElbow @15:39:46.20 SC.reconfigure SCloose @15:39:46.24 SC.hold @15:39:46.29 SC.configure SCloose @15:39:46.37 SE.reconfigure SEconfig @15:39:46.41 SE.hold @15:39:46.46 SE.configure SEconfig @15:39:46.50 CH.coast @15:39:46.54 CH.configure CHconfig @15:39:46.57 PH.coast @15:39:46.62 PH.configure PHconfig @15:39:46.66 PCR.off @15:39:46.69 PCR.pidtemp= 50,0.1,0,300,25,25 @15:39:46.71 PCR.caltemp= 2.7073,0.0373,0 @15:39:46.74 All dwarf controllers running firmware version 3.75 @15:39:46.86 Cleanly exited at 15:38:40CEST08-Aug-18 @15:39:46.89 Loading /home/esp/esp2/mission/stdMER2017.rb mission @15:39:47.42 AFV.select :carrierSolution @15:39:47.52 delay 2 seconds @15:39:49.55 AS.seek 0.5ml @15:40:18.65 delay 1 second @15:40:19.66 AFV.select :holdingCoil @15:40:19.76 ATV.dial :extSrc @15:40:21.07 AS.reconfigure ASslow1 @15:40:21.11 AS.seek 0.53ml @15:40:27.43 delay 5 seconds @15:40:32.51 AS.reconfigure ASslow2 @15:40:32.55 AS.seek 0.54ml @15:40:42.76 delay 20 seconds @15:41:02.83 AS.reconfigure ASslow1 @15:41:02.87 AS.seek 0.57ml @15:41:09.15 delay 5 seconds @15:41:14.23 AS.reconfigure ASslow2 @15:41:14.27 AS.seek 0.58ml @15:41:24.52 delay 20 seconds @15:41:44.59 AS.reconfigure ASslow1 @15:41:44.63 AS.seek 0.61ml @15:41:50.91 delay 5 seconds @15:41:55.99 AS.reconfigure ASslow2 @15:41:56.03 AS.seek 0.62ml @15:42:06.23 delay 20 seconds @15:42:26.30 AS.reconfigure ASslow1 @15:42:26.34 AS.seek 0.65ml @15:42:32.62 delay 5 seconds @15:42:37.70 AS.reconfigure ASslow2 @15:42:37.74 AS.seek 0.66ml @15:42:47.98 delay 20 seconds @15:43:08.02 AS.reconfigure ASslow1 @15:43:08.06 AS.seek 0.705ml @15:43:17.38 delay 10 seconds @15:43:27.40 ATV.dial :air1 @15:43:29.10 AS.seek 0.725ml @15:43:33.40 delay 3 seconds @15:43:36.42 ATV.dial :waste @15:43:36.99 AS.reconfigure ASslow0 @15:43:37.03 AS.seek :empty @15:44:50.47 Setting up PCR for Ss -- writing /var/log/esp/Ss18aug0815h.pcr @15:44:50.50 Building PCR Slug from extSrc with MM1 mix, PP2 primer @15:44:50.51 Priming with carrier @15:44:50.52 ATV.dial ARV @15:44:51.98 ARV.dial :carrier @15:44:52.32 AS.reconfigure ASnorm @15:44:52.36 AS.seek 0.499ml @15:45:17.95 AFV.select :carrierSolution @15:45:18.05 delay 2 seconds @15:45:20.08 AS.reconfigure ASconfig @15:45:20.12 AS.seek 1ml @15:45:49.20 delay 1 second @15:45:50.22 AFV.select :holdingCoil @15:45:50.32 delay 1 second @15:45:51.34 ATV.dial :waste @15:45:52.98 AS.reconfigure ASnorm @15:45:53.03 AS.seek :empty @15:46:43.76 AFV.select :carrierSolution @15:46:43.86 delay 2 seconds @15:46:45.89 AS.reconfigure ASconfig @15:46:45.93 AS.seek 0.5ml @15:47:15.02 delay 1 second @15:47:16.04 AFV.select :holdingCoil @15:47:16.15 Building Slug @15:47:16.16 ATV.dial :ARV @15:47:17.76 delay 2 seconds @15:47:19.78 ARV.dial :air1 @15:47:20.21 delay 2 seconds @15:47:22.28 AS.reconfigure ASslow2 @15:47:22.33 AS.seek 0.506ml @15:47:28.54 delay 2 seconds @15:47:30.56 ARV.dial :MM1 @15:47:31.24 delay 2 seconds @15:47:33.26 Adding MM1 @15:47:33.32 AS.seek 0.524ml @15:47:51.54 delay 25 seconds @15:48:16.56 ARV.dial :PP2 @15:48:16.95 Adding PP2 @15:48:16.96 delay 2 seconds @15:48:19.03 AS.seek 0.53ml @15:48:25.26 delay 10 seconds @15:48:35.29 ARV.dial :air2 @15:48:35.68 adding ARV air @15:48:35.69 delay 2 seconds @15:48:37.76 AS.seek 0.536ml @15:48:43.95 delay 5 seconds @15:48:48.97 ARV.dial :carrier @15:48:50.26 delay 7 seconds @15:48:57.34 AS.seek 0.594ml @15:49:55.98 delay 5 seconds @15:50:01.00 ATV.dial :extSrc @15:50:01.54 delay 2 seconds @15:50:03.61 AS.seek 0.6ml @15:50:10.04 delay 4 seconds @15:50:14.06 ATV.dial ARV @15:50:14.70 delay 2 seconds @15:50:16.72 ARV.dial :carrier @15:50:16.73 delay 2 seconds @15:50:18.80 AS.seek 0.65ml @15:51:09.23 delay 4 seconds @15:51:13.25 ATV.dial PCR @15:51:13.99 ASV.dial :waste1 @15:51:14.07 ARV.dial :air1 @15:51:14.47 Positioning Slug by 0.324ml @15:51:14.94 AS.seek 0.326ml @15:56:41.07 delay 5 seconds @15:56:46.09 ATV.dial ARV @15:56:46.85 ASV.dial :PCRlock @15:56:47.24 delay 5 seconds @15:56:52.26 Running Ss Profile60C350 thermocycling @15:56:52.28 PCR.program Profile60C350 @15:56:52.40 Profile60C350 should complete in < 1:48:05 @15:56:52.41 PCR.run 2:20:06.25 @17:18:09.85 PCR.off @17:18:11.78 PCR for Ss completed -- closed /var/log/esp/Ss18aug0815h.pcr @17:18:11.84 Cleaning PCR @17:18:11.85 AFV.select :carrierSolution @17:18:11.95 delay 2 seconds @17:18:13.98 AS.reconfigure ASconfig @17:18:14.02 AS.seek 0.5ml @17:18:24.32 delay 1 second @17:18:25.34 AFV.select :holdingCoil @17:18:25.43 ASV.dial :waste1 @17:18:25.82 ATV.dial :air3 @17:18:27.09 AS.reconfigure ASslow1 @17:18:27.14 AS.seek 0.51ml @17:18:29.44 delay 2 seconds @17:18:31.45 ATV.dial :bleach @17:18:32.07 AS.seek 0.81ml @17:19:32.67 delay 3 seconds @17:19:35.69 ATV.dial :air3 @17:19:36.23 delay 3 seconds @17:19:39.30 AS.seek 0.835ml @17:19:44.60 delay 3 seconds @17:19:47.62 ATV.dial :bleach @17:19:48.23 AS.seek 0.82ml @17:19:51.48 delay 3 seconds @17:19:54.50 ARV.dial :waste @17:19:55.11 delay 3 seconds @17:19:58.13 ATV.dial :ARV @17:19:59.09 AS.reconfigure ASconfig @17:19:59.14 AS.seek 0.47ml @17:20:19.53 AFV.select :carrierSolution @17:20:19.63 delay 2 seconds @17:20:21.66 AS.seek 1ml @17:20:52.39 delay 1 second @17:20:53.41 AFV.select :holdingCoil @17:20:53.51 delay 1 second @17:20:54.54 AS.reconfigure ASfast @17:20:54.58 AS.seek 0ml @17:21:22.14 AFV.select :carrierSolution @17:21:22.24 delay 2 seconds @17:21:24.65 AS.reconfigure ASconfig @17:21:24.69 AS.seek 1ml @17:22:22.45 delay 1 second @17:22:23.47 AFV.select :holdingCoil @17:22:23.57 delay 1 second @17:22:24.60 AS.reconfigure ASfast @17:22:24.64 AS.seek 0ml @17:22:52.34 AFV.select :carrierSolution @17:22:52.44 delay 2 seconds @17:22:54.47 AS.reconfigure ASconfig @17:22:54.51 AS.seek 1ml @17:23:52.28 delay 1 second @17:23:53.30 AFV.select :holdingCoil @17:23:53.40 delay 1 second @17:23:54.43 AS.reconfigure ASfast @17:23:54.47 AS.seek 0ml @17:24:22.11 ARV.dial between carrier and air1 @17:24:22.61 ATV.dial :air3 @17:24:23.87 AS.reconfigure ASslow1 @17:24:23.91 AS.seek 0.01ml @17:24:26.20 delay 2 seconds @17:24:28.22 ATV.dial :bleach @17:24:28.82 AS.seek 0.81ml @17:27:09.97 delay 3 seconds @17:27:12.99 ATV.dial :air3 @17:27:13.53 delay 3 seconds @17:27:16.60 AS.seek 0.835ml @17:27:21.89 delay 3 seconds @17:27:24.91 ATV.dial :bleach @17:27:25.51 AS.seek 0.82ml @17:27:28.79 delay 3 seconds @17:27:31.81 ATV.dial PCR @17:27:32.20 AS.reconfigure ASconfig @17:27:32.24 AS.seek :empty @17:28:19.72 PCR.program :bleachProfile @17:28:19.77 PCR program should complete in < 2:45 @17:28:19.78 PCR.run 10:00 @17:31:21.60 PCR.off @17:31:21.63 AFV.select :carrierSolution @17:31:21.72 delay 2 seconds @17:31:23.75 AS.seek 1ml @17:32:21.53 delay 1 second @17:32:22.55 AFV.select :holdingCoil @17:32:22.65 delay 1 second @17:32:23.68 AS.reconfigure ASfast @17:32:23.72 AS.seek 0ml @17:32:51.59 delay 3 seconds @17:32:54.61 AFV.select :carrierSolution @17:32:54.71 delay 2 seconds @17:32:56.74 AS.reconfigure ASconfig @17:32:56.79 AS.seek 1ml @17:33:54.53 delay 1 second @17:33:55.55 AFV.select :holdingCoil @17:33:55.65 delay 1 second @17:33:56.68 AS.reconfigure ASfast @17:33:56.72 AS.seek 0ml @17:34:24.45 delay 3 seconds @17:34:27.47 AFV.select :carrierSolution @17:34:27.57 delay 2 seconds @17:34:29.60 AS.reconfigure ASconfig @17:34:29.65 AS.seek 1ml @17:35:27.42 delay 1 second @17:35:28.44 AFV.select :holdingCoil @17:35:28.54 delay 1 second @17:35:29.57 AS.reconfigure ASfast @17:35:29.61 AS.seek 0ml @17:35:57.30 delay 3 seconds @17:36:00.32 AFV.select :carrierSolution @17:36:00.42 delay 2 seconds @17:36:02.45 AS.reconfigure ASconfig @17:36:02.49 AS.seek 1ml @17:37:00.31 delay 1 second @17:37:01.33 AFV.select :holdingCoil @17:37:01.43 delay 1 second @17:37:02.46 AS.reconfigure ASfast @17:37:02.50 AS.seek 0ml @17:37:30.16 delay 3 seconds @17:37:33.18 AFV.select :carrierSolution @17:37:33.27 delay 2 seconds @17:37:35.30 AS.reconfigure ASconfig @17:37:35.35 AS.seek 1ml @17:38:33.14 delay 1 second @17:38:34.16 AFV.select :holdingCoil @17:38:34.25 delay 1 second @17:38:35.28 AS.reconfigure ASnorm @17:38:35.32 AS.seek 0ml @17:39:26.01
AFV.select :carrierSolution @17:39:26.11 delay 2 seconds @17:39:28.14 AS.reconfigure ASconfig @17:39:28.18 AS.seek 0.5ml @17:39:57.23 delay 1 second @17:39:58.24 AFV.select :holdingCoil @17:39:58.33 ATV.dial :air1 @17:39:59.80 AS.reconfigure ASslow2 @17:39:59.84 AS.seek 0.506ml @17:40:06.08 ATV.dial :extSrc @17:40:07.75 AS.seek 0.512ml @17:40:14.14 delay 5 seconds @17:40:19.16 ATV.dial :air1 @17:40:20.85 AS.reconfigure ASslow1 @17:40:20.89 AS.seek 0.542ml @17:40:27.19 ATV.dial :waste @17:40:27.73 AS.reconfigure ASconfig @17:40:27.77 AS.seek :empty @17:40:59.42 Setting up PCR for Ss -- writing /var/log/esp/Ss18aug0817h.pcr @17:40:59.44 Building PCR Slug from extSrc with MM1 mix, PP2 primer @17:40:59.46 Priming with carrier @17:40:59.47 ATV.dial ARV @17:41:00.92 ARV.dial :carrier @17:41:01.26 AS.reconfigure ASnorm @17:41:01.30 AS.seek 0.499ml @17:41:26.83 AFV.select :carrierSolution @17:41:26.93 delay 2 seconds @17:41:28.96 AS.reconfigure ASconfig @17:41:29.00 AS.seek 1ml @17:41:58.06 delay 1 second @17:41:59.08 AFV.select :holdingCoil @17:41:59.18 delay 1 second @17:42:00.20 ATV.dial :waste @17:42:01.84 AS.reconfigure ASnorm @17:42:01.89 AS.seek :empty @17:42:52.61 AFV.select :carrierSolution @17:42:52.71 delay 2 seconds @17:42:54.74 AS.reconfigure ASconfig @17:42:54.78 AS.seek 0.5ml @17:43:23.89 delay 1 second @17:43:24.91 AFV.select :holdingCoil @17:43:25.02 Building Slug @17:43:25.03 ATV.dial :ARV @17:43:26.62 delay 2 seconds @17:43:28.64 ARV.dial :air1 @17:43:29.04 delay 2 seconds @17:43:31.11 AS.reconfigure ASslow2 @17:43:31.16 AS.seek 0.506ml @17:43:37.42 delay 2 seconds @17:43:39.44 ARV.dial :MM1 @17:43:40.03 delay 2 seconds @17:43:42.05 Adding MM1 @17:43:42.52 AS.seek 0.524ml @17:44:00.81 delay 25 seconds @17:44:25.83 ARV.dial :PP2 @17:44:26.23 Adding PP2 @17:44:26.24 delay 2 seconds @17:44:28.31 AS.seek 0.53ml @17:44:34.64 delay 10 seconds @17:44:44.67 ARV.dial :air2 @17:44:45.05 adding ARV air @17:44:45.06 delay 2 seconds @17:44:47.14 AS.seek 0.536ml @17:44:53.55 delay 5 seconds @17:44:58.57 ARV.dial :carrier @17:44:59.86 delay 7 seconds @17:45:06.94 AS.seek 0.594ml @17:46:05.41 delay 5 seconds @17:46:10.43 ATV.dial :extSrc @17:46:10.97 delay 2 seconds @17:46:13.04 AS.seek 0.6ml @17:46:19.24 delay 4 seconds @17:46:23.26 ATV.dial ARV @17:46:23.91 delay 2 seconds @17:46:25.93 ARV.dial :carrier @17:46:25.94 delay 2 seconds @17:46:28.01 AS.seek 0.65ml @17:47:18.50 delay 4 seconds @17:47:22.52 ATV.dial PCR @17:47:23.27 ASV.dial :waste1 @17:47:23.28 ARV.dial :air1 @17:47:23.68 Positioning Slug by 0.324ml @17:47:23.76 AS.seek 0.326ml @17:52:49.77 delay 5 seconds @17:52:54.79 ATV.dial ARV @17:52:55.55 ASV.dial :PCRlock @17:52:55.94 delay 5 seconds @17:53:00.96 Running Ss Profile60C350 thermocycling @17:53:00.97 PCR.program Profile60C350 @17:53:01.10 Profile60C350 should complete in < 1:48:05 @17:53:01.11 PCR.run 2:20:06.25 @18:48:46.04 Session Begun @18:48:50.00 -> ESP::Mode @18:48:52.81 -> can @18:49:26.12 -> quit @18:49:26.14 Session Ended @19:14:21.48 PCR.off @19:14:23.82 PCR for Ss completed -- closed /var/log/esp/Ss18aug0817h.pcr @19:14:23.88 Cleaning PCR @19:14:23.89 AFV.select :carrierSolution @19:14:23.99 delay 2 seconds @19:14:26.02 AS.reconfigure ASconfig @19:14:26.06 AS.seek 0.5ml @19:14:36.36 delay 1 second @19:14:37.38 AFV.select :holdingCoil @19:14:37.48 ASV.dial :waste1 @19:14:37.88 ATV.dial :air3 @19:14:39.16 AS.reconfigure ASslow1 @19:14:39.20 AS.seek 0.51ml @19:14:41.51 delay 2 seconds @19:14:43.53 ATV.dial :bleach @19:14:44.16 AS.seek 0.81ml @19:15:44.74 delay 3 seconds @19:15:47.76 ATV.dial :air3 @19:15:48.30 delay 3 seconds @19:15:51.37 AS.seek 0.835ml @19:15:56.72 delay 3 seconds @19:15:59.74 ATV.dial :bleach @19:16:00.34 AS.seek 0.82ml @19:16:03.61 delay 3 seconds @19:16:06.63 ARV.dial :waste @19:16:07.25 delay 3 seconds @19:16:10.27 ATV.dial :ARV @19:16:11.13 AS.reconfigure ASconfig @19:16:11.18 AS.seek 0.47ml @19:16:31.58 AFV.select :carrierSolution @19:16:31.68 delay 2 seconds @19:16:33.71 AS.seek 1ml @19:17:04.47 delay 1 second @19:17:05.49 AFV.select :holdingCoil @19:17:05.59 delay 1 second @19:17:06.62 AS.reconfigure ASfast @19:17:06.66 AS.seek 0ml @19:17:34.43 AFV.select :carrierSolution @19:17:34.53 delay 2 seconds @19:17:36.56 AS.reconfigure ASconfig @19:17:36.60 AS.seek 1ml @19:18:34.35 delay 1 second @19:18:35.37 AFV.select :holdingCoil @19:18:35.47 delay 1 second @19:18:36.50 AS.reconfigure ASfast @19:18:36.54 AS.seek 0ml @19:19:04.22 AFV.select :carrierSolution @19:19:04.32 delay 2 seconds @19:19:06.35 AS.reconfigure ASconfig @19:19:06.39 AS.seek 1ml @19:20:04.13 delay 1 second @19:20:05.15 AFV.select :holdingCoil @19:20:05.25 delay 1 second @19:20:06.28 AS.reconfigure ASfast @19:20:06.32 AS.seek 0ml @19:20:33.99 ARV.dial between carrier and air1 @19:20:34.49 ATV.dial :air3 @19:20:35.75 AS.reconfigure ASslow1 @19:20:35.79 AS.seek 0.01ml @19:20:38.16 delay 2 seconds @19:20:40.18 ATV.dial :bleach @19:20:40.78 AS.seek 0.81ml @19:23:22.02 delay 3 seconds @19:23:25.04 ATV.dial :air3 @19:23:25.58 delay 3 seconds @19:23:28.65 AS.seek 0.835ml @19:23:33.94 delay 3 seconds @19:23:36.96 ATV.dial :bleach @19:23:37.56 AS.seek 0.82ml @19:23:40.79 delay 3 seconds @19:23:43.81 ATV.dial PCR @19:23:44.19 AS.reconfigure ASconfig @19:23:44.23 AS.seek :empty @19:24:31.72 PCR.program :bleachProfile @19:24:31.77 PCR program should complete in < 2:45 @19:24:31.78 PCR.run 10:00 @19:27:33.60 PCR.off @19:27:33.63 AFV.select :carrierSolution @19:27:33.73 delay 2 seconds @19:27:35.76 AS.seek 1ml @19:28:33.57 delay 1 second @19:28:34.59 AFV.select :holdingCoil @19:28:34.68 delay 1 second @19:28:35.71 AS.reconfigure ASfast @19:28:35.75 AS.seek 0ml @19:29:03.46 delay 3 seconds @19:29:06.48 AFV.select :carrierSolution @19:29:06.58 delay 2 seconds @19:29:08.61 AS.reconfigure ASconfig @19:29:08.65 AS.seek 1ml @19:30:06.44 delay 1 second @19:30:07.46 AFV.select :holdingCoil @19:30:07.56 delay 1 second @19:30:08.59 AS.reconfigure ASfast @19:30:08.63 AS.seek 0ml @19:30:36.49 delay 3 seconds @19:30:39.51 AFV.select :carrierSolution @19:30:39.60 delay 2 seconds @19:30:41.63 AS.reconfigure ASconfig @19:30:41.68 AS.seek 1ml @19:31:39.43 delay 1 second @19:31:40.45 AFV.select :holdingCoil @19:31:40.54 delay 1 second @19:31:41.57 AS.reconfigure ASfast @19:31:41.61 AS.seek 0ml @19:32:09.27 delay 3 seconds @19:32:12.29 AFV.select :carrierSolution @19:32:12.39 delay 2 seconds @19:32:14.42 AS.reconfigure ASconfig @19:32:14.46 AS.seek 1ml @19:33:12.21 delay 1 second @19:33:13.23 AFV.select :holdingCoil @19:33:13.32 delay 1 second @19:33:14.35 AS.reconfigure ASfast @19:33:14.39 AS.seek 0ml @19:33:42.01 delay 3 seconds @19:33:45.03 AFV.select :carrierSolution @19:33:45.13 delay 2 seconds @19:33:47.16 AS.reconfigure ASconfig @19:33:47.20 AS.seek 1ml @19:34:44.99 delay 1 second @19:34:46.01 AFV.select :holdingCoil @19:34:46.10 delay 1 second @19:34:47.13 AS.reconfigure ASnorm @19:34:47.17 AS.seek 0ml @19:35:37.89
AFV.select :carrierSolution @19:35:37.99 delay 2 seconds @19:35:40.02 AS.reconfigure ASconfig @19:35:40.06 AS.seek 0.5ml @19:36:09.13 delay 1 second @19:36:10.15 AFV.select :holdingCoil @19:36:10.24 ATV.dial :air1 @19:36:11.72 AS.reconfigure ASslow2 @19:36:11.76 AS.seek 0.51ml @19:36:22.59 delay 5 seconds @19:36:27.61 ATV.dial :extSrc @19:36:29.29 AS.seek 0.53ml @19:36:49.65 delay 10 seconds @19:36:59.67 ATV.dial :air1 @19:37:01.36 AS.reconfigure ASslow1 @19:37:01.40 AS.seek 0.56ml @19:37:07.73 ATV.dial :waste @19:37:08.27 AS.reconfigure ASconfig @19:37:08.31 AS.seek :empty @19:37:40.83 AFV.select :carrierSolution @19:37:40.93 delay 2 seconds @19:37:42.96 AS.seek 0.5ml @19:38:12.02 delay 1 second @19:38:13.04 AFV.select :holdingCoil @19:38:13.14 ATV.dial :air1 @19:38:13.76 AS.reconfigure ASslow1 @19:38:13.80 AS.seek 0.52ml @19:38:18.10 delay 5 seconds @19:38:23.12 ATV.dial :extSrc @19:38:24.80 AS.reconfigure ASslow2 @19:38:24.84 AS.seek 0.545ml @19:38:50.30 delay 10 seconds @19:39:00.32 ATV.dial :air1 @19:39:02.01 AS.reconfigure ASslow1 @19:39:02.05 AS.seek 0.575ml @19:39:08.40 ATV.dial :waste @19:39:09.04 AS.reconfigure ASconfig @19:39:09.08 AS.seek :empty @19:39:42.49 AFV.select :carrierSolution @19:39:42.58 delay 2 seconds @19:39:44.61 AS.seek 0.5ml @19:40:13.71 delay 1 second @19:40:14.73 AFV.select :holdingCoil @19:40:14.82 ATV.dial :air1 @19:40:15.40 AS.reconfigure ASslow1 @19:40:15.45 AS.seek 0.52ml @19:40:19.76 delay 5 seconds @19:40:24.78 ATV.dial :extSrc @19:40:26.56 AS.reconfigure ASslow2 @19:40:26.61 AS.seek 0.538ml @19:40:44.91 delay 10 seconds @19:40:54.93 ATV.dial :air1 @19:40:56.59 AS.reconfigure ASslow1 @19:40:56.64 AS.seek 0.568ml @19:41:02.90 ATV.dial :waste @19:41:03.54 AS.reconfigure ASconfig @19:41:03.58 AS.seek :empty @19:41:36.59 deconEluateRecoveryLine extSrc @19:41:36.60 ATV.dial :waste @19:41:36.61 AS.reconfigure ASnorm @19:41:36.65 AS.seek 0ml @19:41:36.96 ATV.dial :air3 @19:41:37.63 AS.reconfigure ASslow1 @19:41:37.67 AS.seek 0.01ml @19:41:39.98 delay 2 seconds @19:41:42.00 ATV.dial :bleach @19:41:42.44 AS.seek 0.31ml @19:42:43.04 delay 3 seconds @19:42:46.06 ATV.dial :air3 @19:42:46.60 delay 3 seconds @19:42:49.67 AS.seek 0.335ml @19:42:54.96 delay 3 seconds @19:42:57.98 ATV.dial :bleach @19:42:58.58 AS.seek 0.32ml @19:43:01.87 delay 3 seconds @19:43:04.89 ATV.dial :extSrc @19:43:05.49 AS.seek 0ml @19:44:10.06 AFV.select :carrierSolution @19:44:10.16 delay 2 seconds @19:44:12.19 AS.reconfigure ASconfig @19:44:12.23 AS.seek 0.5ml @19:44:41.29 delay 1 second @19:44:42.31 AFV.select :holdingCoil @19:44:42.41 ATV.dial :extSrc @19:44:42.43 AS.reconfigure ASslow1 @19:44:42.47 AS.seek 0ml @19:46:23.29 AFV.select :carrierSolution @19:46:23.39 delay 2 seconds @19:46:25.42 AS.reconfigure ASconfig @19:46:25.46 AS.seek 0.5ml @19:46:54.49 delay 1 second @19:46:55.51 AFV.select :holdingCoil @19:46:55.61 ATV.dial :extSrc @19:46:55.63 AS.reconfigure ASslow1 @19:46:55.67 AS.seek 0ml @19:48:36.43 AFV.select :carrierSolution @19:48:36.53 delay 2 seconds @19:48:38.56 AS.reconfigure ASconfig @19:48:38.60 AS.seek 0.5ml @19:49:07.67 delay 1 second @19:49:08.69 AFV.select :holdingCoil @19:49:08.79 ATV.dial :extSrc @19:49:08.81 AS.reconfigure ASslow1 @19:49:08.85 AS.seek 0ml @19:50:49.62 AFV.select :carrierSolution @19:50:49.72 delay 2 seconds @19:50:51.75 AS.reconfigure ASconfig @19:50:51.79 AS.seek 0.5ml @19:51:20.85 delay 1 second @19:51:21.87 AFV.select :holdingCoil @19:51:21.97 ATV.dial :extSrc @19:51:21.99 AS.reconfigure ASslow1 @19:51:22.03 AS.seek 0ml @19:53:02.85 ATV.dial :air2 @19:53:04.16 AS.reconfigure ASslow0 @19:53:04.20 AS.seek 0.5ml @19:53:54.81 ATV.dial :extSrc @19:53:56.62 AS.seek 0.02ml @19:54:45.18 delay 1 second @19:54:46.20 ATV.dial :waste @19:54:47.46 AS.reconfigure ASconfig @19:54:47.50 AS.seek :empty @19:54:48.98 deconEluateRecoveryLine extSrc @19:54:48.99 ATV.dial :waste @19:54:49.00 AS.reconfigure ASnorm @19:54:49.04 AS.seek 0ml @19:54:49.37 ATV.dial :air3 @19:54:50.21 AS.reconfigure ASslow1 @19:54:50.25 AS.seek 0.01ml @19:54:52.53 delay 2 seconds @19:54:54.55 ATV.dial :bleach @19:54:54.99 AS.seek 0.31ml @19:55:55.59 delay 3 seconds @19:55:58.61 ATV.dial :air3 @19:55:59.15 delay 3 seconds @19:56:02.22 AS.seek 0.335ml @19:56:07.55 delay 3 seconds @19:56:10.57 ATV.dial :bleach @19:56:11.18 AS.seek 0.32ml @19:56:14.43 delay 3 seconds @19:56:17.45 ATV.dial :extSrc @19:56:18.07 AS.seek 0ml @19:57:22.68 AFV.select :carrierSolution @19:57:22.78 delay 2 seconds @19:57:24.81 AS.reconfigure ASconfig @19:57:24.85 AS.seek 0.5ml @19:57:53.89 delay 1 second @19:57:54.91 AFV.select :holdingCoil @19:57:55.01 ATV.dial :extSrc @19:57:55.03 AS.reconfigure ASslow1 @19:57:55.07 AS.seek 0ml @19:59:35.87 AFV.select :carrierSolution @19:59:35.97 delay 2 seconds @19:59:38.00 AS.reconfigure ASconfig @19:59:38.04 AS.seek 0.5ml @20:00:07.07 delay 1 second @20:00:08.09 AFV.select :holdingCoil @20:00:08.19 ATV.dial :extSrc @20:00:08.21 AS.reconfigure ASslow1 @20:00:08.25 AS.seek 0ml @20:01:49.01 AFV.select :carrierSolution @20:01:49.11 delay 2 seconds @20:01:51.14 AS.reconfigure ASconfig @20:01:51.18 AS.seek 0.5ml @20:02:20.23 delay 1 second @20:02:21.25 AFV.select :holdingCoil @20:02:21.35 ATV.dial :extSrc @20:02:21.37 AS.reconfigure ASslow1 @20:02:21.41 AS.seek 0ml @20:04:02.20 AFV.select :carrierSolution @20:04:02.30 delay 2 seconds @20:04:04.33 AS.reconfigure ASconfig @20:04:04.37 AS.seek 0.5ml @20:04:33.43 delay 1 second @20:04:34.45 AFV.select :holdingCoil @20:04:34.55 ATV.dial :extSrc @20:04:34.57 AS.reconfigure ASslow1 @20:04:34.61 AS.seek 0ml @20:06:15.40 ATV.dial :air2 @20:06:16.70 AS.reconfigure ASslow0 @20:06:16.74 AS.seek 0.5ml @20:07:07.28 ATV.dial :extSrc @20:07:08.84 AS.seek 0.02ml @20:07:57.40 delay 1 second @20:07:58.42 ATV.dial :waste @20:07:59.70 AS.reconfigure ASconfig @20:07:59.74 AS.seek :empty @20:08:01.23 deconEluateRecoveryLine extSrc @20:08:01.24 ATV.dial :waste @20:08:01.25 AS.reconfigure ASnorm @20:08:01.29 AS.seek 0ml @20:08:01.67 ATV.dial :air3 @20:08:02.51 AS.reconfigure ASslow1 @20:08:02.55 AS.seek 0.01ml @20:08:04.81 delay 2 seconds @20:08:06.83 ATV.dial :bleach @20:08:07.28 AS.seek 0.31ml @20:09:07.87 delay 3 seconds @20:09:10.89 ATV.dial :air3 @20:09:11.44 delay 3 seconds @20:09:14.51 AS.seek 0.335ml @20:09:19.85 delay 3 seconds @20:09:22.87 ATV.dial :bleach @20:09:23.48 AS.seek 0.32ml @20:09:26.78 delay 3 seconds @20:09:29.80 ATV.dial :extSrc @20:09:30.51 AS.seek 0ml @20:10:35.12 AFV.select :carrierSolution @20:10:35.22 delay 2 seconds @20:10:37.25 AS.reconfigure ASconfig @20:10:37.29 AS.seek 0.5ml @20:11:06.33 delay 1 second @20:11:07.35 AFV.select :holdingCoil @20:11:07.45 ATV.dial :extSrc @20:11:07.47 AS.reconfigure ASslow1 @20:11:07.52 AS.seek 0ml @20:12:48.33 AFV.select :carrierSolution @20:12:48.43 delay 2 seconds @20:12:50.46 AS.reconfigure ASconfig @20:12:50.50 AS.seek 0.5ml @20:13:19.53 delay 1 second @20:13:20.55 AFV.select :holdingCoil @20:13:20.65 ATV.dial :extSrc @20:13:20.67 AS.reconfigure ASslow1 @20:13:20.71 AS.seek 0ml @20:15:01.48 AFV.select :carrierSolution @20:15:01.58 delay 2 seconds @20:15:03.61 AS.reconfigure ASconfig @20:15:03.65 AS.seek 0.5ml @20:15:33.08 delay 1 second @20:15:34.10 AFV.select :holdingCoil @20:15:34.20 ATV.dial :extSrc @20:15:34.22 AS.reconfigure ASslow1 @20:15:34.26 AS.seek 0ml @20:17:15.08 AFV.select :carrierSolution @20:17:15.18 delay 2 seconds @20:17:17.21 AS.reconfigure ASconfig @20:17:17.25 AS.seek 0.5ml @20:17:46.28 delay 1 second @20:17:47.30 AFV.select :holdingCoil @20:17:47.40 ATV.dial :extSrc @20:17:47.42 AS.reconfigure ASslow1 @20:17:47.46 AS.seek 0ml @20:19:28.28 ATV.dial :air2 @20:19:29.59 AS.reconfigure ASslow0 @20:19:29.63 AS.seek 0.5ml @20:20:20.19 ATV.dial :extSrc @20:20:21.75 AS.seek 0.02ml @20:21:10.36 delay 1 second @20:21:11.38 ATV.dial :waste @20:21:12.66 AS.reconfigure ASconfig @20:21:12.70 AS.seek :empty No '18run.rb' script found on ESPpath: /home/esp/esp2/mission:. @12:48:53.04CEST15-Aug-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @12:48:55.37 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @12:48:55.64 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @12:49:01.19 Polling Can every 10:00 @12:49:03.38 SPE.coast @12:49:03.42 SPE.configure SPEconfig @12:49:03.52 AS.reconfigure ASconfig @12:49:03.56 AS.hold @12:49:03.61 AS.configure ASconfig @12:49:03.70 SS.reconfigure SSconfig @12:49:03.74 SS.hold @12:49:03.78 SS.configure SSconfig @12:49:03.86 CC.reconfigure CCconfig @12:49:03.90 CC.hold @12:49:03.98 CC.configure CCconfig @12:49:04.06 CS.reconfigure CSconfig @12:49:04.10 CS.hold @12:49:04.16 CS.configure CSconfig @12:49:04.70 PC.reconfigure PCconfig @12:49:04.77 PC.hold @12:49:04.87 PC.configure PCconfig @12:49:05.04 PS.configure PSconfig @12:49:05.23 Forearm.reconfigure LooseForearm @12:49:05.31 Forearm.hold @12:49:05.36 Forearm.configure LooseForearm @12:49:05.44 Elbow.reconfigure LooseElbow @12:49:05.48 Elbow.hold @12:49:05.53 Elbow.configure LooseElbow @12:49:05.63 SC.reconfigure SCloose @12:49:05.67 SC.hold @12:49:05.73 SC.configure SCloose @12:49:05.82 SE.reconfigure SEconfig @12:49:05.86 SE.hold @12:49:05.91 SE.configure SEconfig @12:49:05.95 CH.coast @12:49:06.00 CH.configure CHconfig @12:49:06.02 PH.coast @12:49:06.07 PH.configure PHconfig @12:49:06.11 PCR.off @12:49:06.15 PCR.pidtemp= 50,0.1,0,300,25,25 @12:49:06.19 PCR.caltemp= 2.7073,0.0373,0 @12:49:06.23 All dwarf controllers running firmware version 3.75 @12:49:06.39 Cleanly exited at 12:47:22CEST15-Aug-18 @12:49:06.45 Loading /home/esp/esp2/mission/18tun.rb mission @12:49:06.59 Preparing to sleep until +0:00:15 @12:49:06.61 GoodNews.email "Starting 18tun w/pucks in tube 2\n{3 => 0, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\nCan@12:49:01, 16.4C, 66% humidity, 14.6psia, 12.006V, 0.626A, 0.626A avg, 7.51W",Subject: "18tun begun" @12:49:06.75 AS.coast @12:49:06.81
Asleep til +0:00:15 @12:49:06.86 Continuing at 12:49 @12:49:06.91 SS.coast @12:49:07.10 CC.coast @12:49:07.16 Session Begun @12:49:07.24 CS.coast @12:49:07.32 PC.coast @12:49:07.38 Forearm.coast @12:49:07.45 Elbow.coast @12:49:07.51 SC.coast @12:49:07.58 SE.coast @12:49:08.00 Gate.power :core,:OFF @12:49:08.02 Gate.power :raw,:OFF @12:49:08.04 Shutdown @12:49:13.94 GoodNews.email "Then, in tube 2: coreNegNTCphase\n/home/esp/esp2/mission/18tun.rb:106\n{3 => 0, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\nCan@12:49:07, 16.4C, 66% humidity, 14.6psia, 12.011V, 0.619A, 0.626A avg, 7.44W",Subject: "Asleep until 12:49" @12:49:21.46
Powering core bus @12:49:24.50 I2C::DuplicateAddress::Error in core -- Multiple core microcontrollers at address 0x27 @12:49:26.48 Gate.power :raw,:ON @12:49:27.07 SPE.coast @12:49:27.14 SPE.configure SPEconfig @12:49:27.24 AS.configure ASconfig @12:49:27.34 SS.configure SSconfig @12:49:27.45 CC.configure CCconfig @12:49:27.55 CS.configure CSconfig @12:49:27.66 PC.configure PCconfig @12:49:27.75 PS.configure PSconfig @12:49:27.82 I2C::NodeOffline in MAIN -- manipulator Not Responding @12:49:27.84 MISSING Hand @12:49:27.87 I2C::NodeOffline in MAIN -- manipulator Not Responding @12:49:27.90 MISSING Forearm @12:49:27.93 I2C::NodeOffline in MAIN -- manipulator Not Responding @12:49:27.96 MISSING Elbow @12:49:28.06 SC.configure SCloose @12:49:28.16 SE.configure SEconfig @12:49:28.21 CH.coast @12:49:28.27 CH.configure CHconfig @12:49:28.32 PH.coast @12:49:28.39 PH.configure PHconfig @12:49:28.44 PCR.off @12:49:28.48 PCR.pidtemp= 50,0.1,0,300,25,25 @12:49:28.52 PCR.caltemp= 2.7073,0.0373,0 @12:49:28.55 All dwarf controllers running firmware version 3.75 @12:49:28.68 Valve.reset @12:49:28.85 PCR.off @12:49:28.89 PCR.pidtemp= 50,0.1,0,300,25,25 @12:49:28.92 PCR.caltemp= 2.7073,0.0373,0 @12:49:29.05 Intake.select :close @12:49:29.08 I2C::NodeOffline in MAIN -- manipulator microcontroller never signed on while reading Forearm's status @12:49:29.12 Exhaust.select :close @12:49:29.16
Waiting to Resume from Checkpoint "Continuing at 12:49" @12:49:29.18 BadNews.email "manipulator microcontroller never signed on while reading Forearm's status",Subject: "I2C::NodeOffline in MAIN" @12:49:29.20 CTV.dial :air @12:49:29.30 PSV.dial :air @12:49:29.32 CSV.dial :waste1 @12:49:29.45 PS.reconfigure PSconfig @12:49:29.53 PS.home.seek 0ml @12:49:29.55 SSV.dial :spit,avoiding: :exhaust @12:49:30.10 CS.reconfigure CSfast @12:49:30.13 SS.reconfigure SSconfig @12:49:30.24 CS.home.seek 0ml @12:49:30.30 SS.home.seek 0ml @12:49:39.10 PS.coast @12:49:59.86 -> abort! @12:49:59.88 CmdServer::Client::Error in bkha -- Attempt to Abort Mission without specifying a reason! @12:50:10.97 -> abort! "hand not responding again" @12:50:10.98 CmdServer::Client::Error in bkha -- Aborting Mission: hand not responding again @12:50:11.03 BadNews.email "hand not responding again",Subject: "18tun ABORTED!" @12:50:17.42 Session Ended @12:59:55.66CEST15-Aug-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @12:59:57.90 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @12:59:58.15 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @13:00:04.07 Polling Can every 10:00 @13:00:06.27 SPE.coast @13:00:06.31 SPE.configure SPEconfig @13:00:06.41 AS.reconfigure ASconfig @13:00:06.44 AS.hold @13:00:06.49 AS.configure ASconfig @13:00:06.58 SS.reconfigure SSconfig @13:00:06.62 SS.hold @13:00:06.66 SS.configure SSconfig @13:00:06.75 CC.reconfigure CCconfig @13:00:06.79 CC.hold @13:00:06.85 CC.configure CCconfig @13:00:06.96 CS.reconfigure CSconfig @13:00:07.04 CS.hold @13:00:07.12 CS.configure CSconfig @13:00:07.85 PC.reconfigure PCconfig @13:00:07.89 PC.hold @13:00:07.93 PC.configure PCconfig @13:00:08.01 PS.configure PSconfig @13:00:08.12 Forearm.reconfigure LooseForearm @13:00:08.16 Forearm.hold @13:00:08.20 Forearm.configure LooseForearm @13:00:08.28 Elbow.reconfigure LooseElbow @13:00:08.32 Elbow.hold @13:00:08.38 Elbow.configure LooseElbow @13:00:08.59 SC.reconfigure SCloose @13:00:08.66 SC.hold @13:00:08.76 SC.configure SCloose @13:00:08.93 SE.reconfigure SEconfig @13:00:09.00 SE.hold @13:00:09.10 SE.configure SEconfig @13:00:09.18 CH.coast @13:00:09.23 CH.configure CHconfig @13:00:09.26 PH.coast @13:00:09.31 PH.configure PHconfig @13:00:09.34 PCR.off @13:00:09.37 PCR.pidtemp= 50,0.1,0,300,25,25 @13:00:09.40 PCR.caltemp= 2.7073,0.0373,0 @13:00:09.43 All dwarf controllers running firmware version 3.75 @13:00:09.54 Cleanly exited at 12:59:36CEST15-Aug-18 @13:00:09.58 Loading /home/esp/esp2/mission/18tun.rb mission @13:00:09.63 Preparing to sleep until +0:00:15 @13:00:09.65 GoodNews.email "Starting 18tun w/pucks in tube 2\n{3 => 0, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\nCan@13:00:04, 16.7C, 45% humidity, 14.6psia, 12.001V, 0.651A, 0.645A avg, 7.81W",Subject: "18tun begun" @13:00:09.72 AS.coast @13:00:09.75
Asleep til +0:00:15 @13:00:09.79 Continuing at 13:00 @13:00:09.83 SS.coast @13:00:10.03 CC.coast @13:00:10.09 CS.coast @13:00:10.15 PC.coast @13:00:10.21 Forearm.coast @13:00:10.28 Elbow.coast @13:00:10.36 SC.coast @13:00:10.42 SE.coast @13:00:10.67 Session Begun @13:00:10.88 Gate.power :core,:OFF @13:00:10.91 Gate.power :raw,:OFF @13:00:10.93 Shutdown @13:00:16.48 GoodNews.email "Then, in tube 2: coreNegNTCphase\n/home/esp/esp2/mission/18tun.rb:106\n{3 => 0, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\nCan@13:00:09, 16.6C, 45% humidity, 14.6psia, 12.001V, 0.645A, 0.645A avg, 7.74W",Subject: "Asleep until 13:00" @13:00:24.61
Powering core bus @13:00:29.27 Gate.power :raw,:ON @13:00:29.91 SPE.coast @13:00:29.98 SPE.configure SPEconfig @13:00:30.08 AS.configure ASconfig @13:00:30.18 SS.configure SSconfig @13:00:30.28 CC.configure CCconfig @13:00:30.38 CS.configure CSconfig @13:00:30.49 PC.configure PCconfig @13:00:30.58 PS.configure PSconfig @13:00:30.71 Forearm.configure LooseForearm @13:00:30.81 Elbow.configure LooseElbow @13:00:30.93 SC.configure SCloose @13:00:31.04 SE.configure SEconfig @13:00:31.09 CH.coast @13:00:31.16 CH.configure CHconfig @13:00:31.20 PH.coast @13:00:31.27 PH.configure PHconfig @13:00:31.33 PCR.off @13:00:31.36 PCR.pidtemp= 50,0.1,0,300,25,25 @13:00:31.40 PCR.caltemp= 2.7073,0.0373,0 @13:00:31.44 All dwarf controllers running firmware version 3.75 @13:00:31.56 Valve.reset @13:00:31.73 PCR.off @13:00:31.77 PCR.pidtemp= 50,0.1,0,300,25,25 @13:00:31.80 PCR.caltemp= 2.7073,0.0373,0 @13:00:31.99 PSV.dial :air @13:00:32.02 Intake.select :close @13:00:32.07 Exhaust.select :close @13:00:32.09 CTV.dial :air @13:00:32.20 PS.reconfigure PSconfig @13:00:32.32 PS.home.seek 0ml @13:00:32.36
Forearm.reconfigure LooseForearm @13:00:32.39 CSV.dial :waste1 @13:00:32.83
Forearm.home.seek :retract @13:00:32.97 SSV.dial :spit,avoiding: :exhaust @13:00:33.11 CS.reconfigure CSfast @13:00:33.14 SS.reconfigure SSconfig @13:00:33.24 CS.home.seek 0ml @13:00:33.27 SS.home.seek 0ml @13:00:33.65 Hand.close @13:00:33.70
Elbow.jog 1500 @13:00:34.23 Elbow.reconfigure LooseElbow @13:00:34.29 Elbow.home.seek :clear @13:00:39.01 SE.reconfigure SEconfig @13:00:39.07 SE.home.seek :down @13:00:39.68 SC.reconfigure SCsloppy @13:00:39.74 SC.home.seek :initial @13:00:41.74 PS.coast @13:00:43.82
Forearm.seek :retract @13:00:43.92 Elbow.seek :hammer @13:00:45.15 Forearm.reconfigure TightForearm @13:00:45.22 Forearm.seek 5 + 350 counts @13:00:46.27 Forearm.seek :retract @13:00:47.46 CC.reconfigure CCconfig @13:00:47.49 PC.reconfigure PCconfig @13:00:47.59
CC.home.seek :open @13:00:47.62 PC.home.seek :open @13:00:50.94
Phase [coreNegNTCphase] @13:00:50.95 GoodNews.email "At 13:00:50 began in tube 2: coreNegNTCphase\n/home/esp/esp2/mission/18tun.rb:106\n{3 => 0, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\nCan@13:00:50, 16.6C, 45% humidity, 14.6psia, 11.977V, 0.758A, 0.758A avg, 9.08W",Subject: "Started 18tun phase coreNegNTCphase" @13:00:51.00CEST15-Aug-18
==================> STARTED coreNegNTC (phase #1) <=== @13:00:51.02 Starting initialPurge @13:00:51.04 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:00:51.13 Hand.open @13:00:51.99 Elbow.reconfigure TightElbow @13:00:52.05 Elbow.seek FlushPuck::Garage @13:00:54.11 Elbow.reconfigure LooseElbow @13:00:54.21 Forearm.reconfigure LooseForearm @13:00:54.28 Forearm.seek :garage @13:00:55.32 Hand.close @13:00:56.24 Forearm.seek :retract @13:00:57.26 Elbow.reconfigure TightElbow @13:00:57.33 Elbow.seek CC @13:01:00.31 Elbow.reconfigure LooseElbow @13:01:00.42 Forearm.reconfigure TightForearm @13:01:00.49 Forearm.seek CC @13:01:01.70 CC.seek :closed @13:01:05.95 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.36 @13:01:06.03 Hand.open @13:01:06.82 Forearm.reconfigure LooseForearm @13:01:06.88 Forearm.seek :retract @13:01:07.87 Flushing the Collection Valve::Manifold @13:01:08.01 CSV.dial CSR @13:01:09.59 Pulling 5ml of CSR.flush @13:01:09.60 CSR.select :flush @13:01:09.73 CS.reconfigure CSconfig @13:01:09.79 CS.seek 5ml @13:01:23.65 delay 5 seconds @13:01:28.67 CSR.select :air @13:01:28.85 CS.reconfigure CSfast @13:01:28.90 CS.seek 7.5ml @13:01:36.55 CSV.dial :waste @13:01:38.07 CS.reconfigure CSconfig @13:01:38.13 CS.seek 0ml @13:01:58.33 Purging external Intake @13:01:58.46 CSV.dial :flush @13:01:58.99 CS.reconfigure CSfast @13:01:59.05 CS.seek 10ml @13:02:25.86 delay 5 seconds @13:02:30.88 CSV.dial CBV @13:02:30.92
CBV.dial CSV @13:02:31.82 CTV.dial :intake @13:02:32.71 Intake.select :open @13:02:32.83 CS.seek 0ml @13:03:00.70 Intake.select :close @13:03:00.82 CTV.dial :air @13:03:01.71 Flushing upper manifold @13:03:01.84 CSV.dial :flush @13:03:02.69 CS.seek 5ml @13:03:16.55 delay 5 seconds @13:03:21.57 CSV.dial :air @13:03:22.16 CS.seek 7ml @13:03:28.33 CSV.dial CBV @13:03:28.36
CBV.dial CSV @13:03:29.57 CTV.dial :air @13:03:29.59 CS.seek 0ml @13:03:48.46 delay 7 seconds @13:03:55.50 CS.seek 3ml @13:04:04.20 CSV.dial :waste @13:04:04.74 CS.seek 0ml @13:04:13.50 CSV.dial :flush @13:04:14.40 CS.seek 3ml @13:04:23.05 delay 5 seconds @13:04:28.07 CSV.dial :air @13:04:28.65 CS.seek 5ml @13:04:34.74 CSV.dial :air @13:04:34.76 CS.seek 0ml @13:04:48.55 delay 7 seconds @13:04:55.59 CS.seek 3ml @13:05:04.24 CSV.dial :waste @13:05:05.10 CS.seek 0ml @13:05:13.77 Garaging FlushPuck in Collection Clamp @13:05:13.86 Elbow.reconfigure TightElbow @13:05:13.92 Elbow.seek CC @13:05:14.17 Elbow.reconfigure LooseElbow @13:05:14.28 Forearm.seek CC @13:05:15.24 Hand.close @13:05:16.01 CC.seek :open @13:05:20.38 Forearm.seek :retract @13:05:21.39 Elbow.reconfigure TightElbow @13:05:21.45 Elbow.seek :garage @13:05:24.56 Elbow.reconfigure LooseElbow @13:05:24.67 Forearm.reconfigure TightForearm @13:05:24.73 Forearm.seek :garage @13:05:25.90 Hand.open @13:05:26.70 Forearm.reconfigure LooseForearm @13:05:26.76 Forearm.seek :retract @13:05:27.86 Starting mfb @13:05:27.87 pcrBAC wcr sampling at most 1500ml @13:05:27.92 BACfans.email "Started mfb in tube 2 at 13:05:27CEST15-Aug-18\n{3 => 0, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\nCan@13:00:50, 16.6C, 45% humidity, 14.6psia, 11.977V, 0.758A, 0.758A avg, 9.08W",Subject: "pcrBAC wcr sampling at most 1500ml" @13:05:27.99
Loading sh1 puck from tube 2 into Collection Clamp @13:05:28.12 Readying the MFB @13:05:28.16 Homing MFB Syringe @13:05:28.20 AFV.select :holdingCoil @13:05:28.43 ATV.dial :air2 @13:05:28.54
SC.reconfigure SCloose @13:05:28.85 Elbow.reconfigure TightElbow @13:05:28.91 Elbow.seek 2 @13:05:29.71 AS.reconfigure ASconfig @13:05:29.82 AS.home.seek :home @13:05:31.23
Forearm.reconfigure TightForearm @13:05:31.40 Forearm.seek 2 + 700 counts @13:05:32.51 SE.reconfigure SEpick @13:05:32.68 SE.seek beamBreak @13:05:35.43 AFV.select :carrierSolution @13:05:35.54 delay 2 seconds @13:05:35.84
SE.configure SEconfig @13:05:36.02 Elbow.seek 2 @13:05:36.22 Forearm.seek 2 + 300 counts @13:05:36.48 Forearm.reconfigure LooseForearm @13:05:36.54 Hand.close @13:05:37.29 SE.seek :down @13:05:37.50
Forearm.seek :retract @13:05:37.58 AS.seek 1ml @13:05:38.47
Elbow.seek CC @13:05:40.03 SE.coast @13:05:40.58
Elbow.reconfigure LooseElbow @13:05:40.80 Forearm.reconfigure TightForearm @13:05:40.91 Forearm.seek CC @13:05:42.27 CC.seek :closed @13:05:46.49 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.29 @13:05:46.50 Hand.open @13:05:47.32 Forearm.reconfigure LooseForearm @13:05:47.38 Forearm.seek :retract @13:05:48.40 SC.coast @13:05:48.45 skipping prime and sample collection @13:05:48.47 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @13:05:48.48 Intake.select :close @13:05:48.59 CSV.dial :waste @13:05:48.66 CS.seek 0ml @13:05:48.79
Calibrating pressure and temperature sensors @13:05:48.81 CSV.dial :air @13:05:48.89
CTV.dial :air @13:05:49.01 SSV.dial :spit,avoiding: :exhaust @13:05:49.06 SS.reconfigure SSfast @13:05:49.26 SS.seek 5ml @13:05:49.65 Session Begun @13:05:49.92 CS.seek 10ml @13:05:52.16 SSV.dial CBV @13:05:52.19
CBV.dial SSV @13:05:53.76 delay 3.8 seconds @13:05:57.68 SSV.dial :spit @13:05:59.21 SS.seek 0ml @13:06:01.98 SSV.dial CBV @13:06:03.51 SSV.dial CBV,avoiding: :exhaust @13:06:03.55
CBV.dial SSV @13:06:03.57 CTV.dial :air @13:06:03.62 delay 15 seconds @13:06:18.66 SSV.dial :spit,avoiding: :exhaust @13:06:20.18 CTV.dial CSV,avoiding: :intake @13:06:20.26
SS.reconfigure SSexhale @13:06:20.35 SS.seek 0ml @13:06:20.51 SSV.dial CBV,avoiding: :exhaust @13:06:22.12 CBV.dial between SSV and waste2 @13:06:22.57 CTV.dial CSV,avoiding: :air @13:06:22.60
CSV.dial CTV @13:06:23.16 CS.reconfigure CSpressurize @13:06:23.24 CS.seek 0ml @13:06:30.47 delay 1 second @13:06:31.49 AFV.select :holdingCoil @13:06:31.61 delay 1 second @13:06:32.63 ATV.dial :waste @13:06:33.83 AS.reconfigure ASnorm @13:06:33.92 AS.home.seek :empty @13:06:49.40
CSV.dial :air @13:06:49.95 CS.reconfigure CSfast @13:06:50.03 CS.seek 10ml @13:07:16.42 CSV.dial between air and CTV @13:07:16.82 CS.seek 3.421ml @13:07:26.50 ATV.dial :waste @13:07:26.52 ARV.dial between carrier and air1 @13:07:26.65 ASV.dial :waste1 @13:07:34.68 CTV.dial CSV,avoiding: :air @13:07:34.71
CSV.dial CTV @13:07:35.09 CS.reconfigure CSpressurize @13:07:35.17 CS.seek 0ml @13:07:44.68 CSV.dial :air @13:07:45.23 CS.reconfigure CSfast @13:07:45.31 CS.seek 10ml @13:08:11.70 CSV.dial between air and CTV @13:08:12.52 CS.seek 2.155ml @13:08:33.43 CTV.dial CSV,avoiding: :air @13:08:33.46
CSV.dial CTV @13:08:33.84 CS.reconfigure CSpressurize @13:08:33.92 CS.seek 0ml @13:08:35.91 BubblePoint: Intake @70.0psia, Outlet @14.8psia @13:08:35.92 CBV.dial SSV @13:08:36.31 SS.reconfigure SSconfig @13:08:36.38 SS.seek 10ml @13:08:42.46 delay 15 seconds @13:08:57.58 Evacuated: Intake @28.6psia, Outlet @29.0psia @13:08:57.59 SSV.dial :spit,avoiding: :exhaust @13:08:59.16 SS.reconfigure SSexhale @13:08:59.24 SS.seek 0ml @13:09:04.25 SS.reconfigure SSfast @13:09:04.33 SS.seek 5ml @13:09:07.18 SSV.dial CBV,avoiding: :exhaust @13:09:07.21
CBV.dial SSV @13:09:08.75 CTV.dial :air,avoiding: :intake @13:09:10.34 CSV.dial CTV @13:09:10.38
SS.seek 10ml @13:09:11.38 CSV.dial :air @13:09:11.92 CS.reconfigure CSfast @13:09:11.99 CS.seek 0ml @13:09:13.38
SSV.dial :spit,avoiding: :exhaust @13:09:14.91 SS.reconfigure SSexhale @13:09:14.99 SS.seek 0ml @13:09:17.09 CS.coast @13:09:20.02
SS.reconfigure SSfast @13:09:20.10 SS.seek 5ml @13:09:23.24 SSV.dial CBV,avoiding: :exhaust @13:09:23.30
CBV.dial SSV @13:09:25.78 SSV.dial :spit,avoiding: :exhaust @13:09:27.32 SS.reconfigure SSconfig @13:09:27.40 SS.seek 0ml @13:09:30.47 SS.coast @13:09:30.54 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @13:09:30.55 Intake.select :close @13:09:30.67 CSV.dial :waste @13:09:30.82 SSV.dial CBV,avoiding: :exhaust @13:09:30.85
CBV.dial SSV @13:09:31.58 CS.seek 0ml @13:09:31.67 CSV.dial :air @13:09:32.41
CTV.dial :air @13:09:32.42 delay 15 seconds @13:09:32.54 CS.seek 10ml @13:09:47.47
SSV.dial :spit,avoiding: :exhaust @13:09:49.02 CTV.dial CSV,avoiding: :intake @13:09:49.07
SS.reconfigure SSexhale @13:09:49.19 SS.seek 0ml @13:09:49.69 SSV.dial CBV,avoiding: :exhaust @13:09:59.08 CBV.dial between SSV and waste2 @13:09:59.54 CTV.dial CSV,avoiding: :air @13:09:59.57
CSV.dial CTV @13:10:00.10 CS.reconfigure CSpressurize @13:10:00.18 CS.seek 0ml @13:10:26.30 CSV.dial :air @13:10:26.85 CS.reconfigure CSfast @13:10:26.93 CS.seek 10ml @13:10:53.39 CSV.dial between air and CTV @13:10:53.79 CS.seek 3.39ml @13:11:11.59 CTV.dial CSV,avoiding: :air @13:11:11.62
CSV.dial CTV @13:11:12.00 CS.reconfigure CSpressurize @13:11:12.08 CS.seek 0ml @13:11:21.55 CSV.dial :air @13:11:22.11 CS.reconfigure CSfast @13:11:22.19 CS.seek 10ml @13:11:48.45 CSV.dial between air and CTV @13:11:49.40 CS.seek 2.139ml @13:12:10.40 CTV.dial CSV,avoiding: :air @13:12:10.43
CSV.dial CTV @13:12:10.83 CS.reconfigure CSpressurize @13:12:10.91 CS.seek 0ml @13:12:12.94 BubblePoint: Intake @70.7psia, Outlet @16.3psia @13:12:12.95 CBV.dial SSV @13:12:13.36 SS.reconfigure SSconfig @13:12:13.44 SS.seek 10ml @13:12:19.49 delay 15 seconds @13:12:34.61 Evacuated: Intake @28.9psia, Outlet @30.0psia @13:12:34.62 SSV.dial :spit,avoiding: :exhaust @13:12:36.19 SS.reconfigure SSexhale @13:12:36.26 SS.seek 0ml @13:12:41.28 SS.reconfigure SSfast @13:12:41.36 SS.seek 5ml @13:12:44.40 SSV.dial CBV,avoiding: :exhaust @13:12:44.43
CBV.dial SSV @13:12:45.97 CTV.dial :air,avoiding: :intake @13:12:47.56 CSV.dial CTV @13:12:47.60
SS.seek 10ml @13:12:48.60 CSV.dial :air @13:12:49.14 CS.reconfigure CSfast @13:12:49.21 CS.seek 0ml @13:12:50.37
SSV.dial :spit,avoiding: :exhaust @13:12:51.90 SS.reconfigure SSexhale @13:12:51.98 SS.seek 0ml @13:12:54.27 CS.coast @13:12:57.13
SS.reconfigure SSfast @13:12:57.21 SS.seek 5ml @13:13:00.30 SSV.dial CBV,avoiding: :exhaust @13:13:00.33
CBV.dial SSV @13:13:02.89 SSV.dial :spit,avoiding: :exhaust @13:13:04.41 SS.reconfigure SSconfig @13:13:04.49 SS.seek 0ml @13:13:07.53 SS.coast @13:13:07.59 Lysing: Adding 1.5ml lysis @13:13:07.61 CBV.dial between CSV and waste1,avoiding: :DBV @13:13:09.36 CTV.dial CSV @13:13:10.92 CSV.dial CSR @13:13:11.83 Pulling 1ml of CSR.lysis @13:13:11.84 CSR.select :lysis @13:13:12.00 CS.reconfigure CSconfig @13:13:12.07 CS.seek 1ml @13:13:15.72 delay 5 seconds @13:13:20.74 CSR.select :air @13:13:20.92 CS.seek 6ml @13:13:34.65 delay 2 seconds @13:13:36.67 CSV.dial :waste1 @13:13:38.19 CS.reconfigure CSfast @13:13:38.27 CS.seek :empty @13:13:54.52 CSV.dial CSR @13:13:56.09 Pulling 1.5ml of CSR.lysis @13:13:56.10 CSR.select :lysis @13:13:56.26 CS.reconfigure CSconfig @13:13:56.32 CS.seek 1.5ml @13:14:01.16 delay 5 seconds @13:14:06.18 CSR.select :air @13:14:06.38 CS.seek 4.5ml @13:14:15.12 delay 2 seconds @13:14:17.14 CSV.dial CTV @13:14:17.69 CS.seek 1.275ml @13:14:26.91 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @13:14:26.94 CH.seek 85C,8:00 @13:17:49.93 CH.hold 5:00,85C @13:22:50.10 Collection Puck Heater at 85.2C after lysing @13:22:50.12 CS.seek 0ml @13:22:54.51 CTV.dial between spare and air,avoiding: :intake @13:22:55.75 CSV.dial :air @13:22:56.27 CS.seek 5ml @13:23:10.21 CSV.dial CTV @13:23:10.76 delay 2 seconds @13:23:12.78 CSV.dial :air @13:23:13.33 delay 2 seconds @13:23:15.35 CSV.dial CTV @13:23:15.91 delay 2 seconds @13:23:17.93 CSV.dial :air @13:23:18.47 delay 2 seconds @13:23:20.50 CS.seek 0ml @13:23:34.32 Recovering Lysate into Collection syringe @13:23:34.33 CSV.dial CBV @13:23:35.54 CBV.dial CSV @13:23:35.97 CS.seek 5ml @13:23:49.69 delay 5 seconds @13:23:54.71 CTV.dial :air @13:23:55.30 CS.seek 7ml @13:24:01.57 delay 5 seconds @13:24:06.59 CSV.dial :air2 @13:24:07.12 CS.seek 10ml @13:24:15.79 CSV.dial between air and CTV,avoiding: CBV @13:24:17.24 Lysing Complete @13:24:17.34 Storing sh1 puck in Collection Clamp to tube 1 @13:24:17.52 SC.reconfigure SCtight @13:24:17.59 SC.seek 1 @13:24:19.77 SC.reconfigure SCloose @13:24:20.00 Elbow.reconfigure TightElbow @13:24:20.07 Elbow.seek 1 @13:24:22.01 Forearm.reconfigure TightForearm @13:24:22.08 Forearm.seek 1 + 700 counts @13:24:23.20 SE.reconfigure SEpick @13:24:23.30 SE.seek beamBreak @13:24:26.72 SE.configure SEconfig @13:24:26.87 SE.seek :place @13:24:27.39 Forearm.reconfigure LooseForearm @13:24:27.47 Forearm.seek :retract @13:24:28.42 Elbow.seek CC @13:24:30.14 Elbow.reconfigure LooseElbow @13:24:30.25 Forearm.seek CC @13:24:31.28 Hand.close @13:24:32.03 CC.seek :open @13:24:36.54 Forearm.seek :retract @13:24:37.57 Elbow.reconfigure TightElbow @13:24:37.64 Elbow.seek 1 @13:24:39.58 Elbow.reconfigure LimpElbow @13:24:39.66 Forearm.seek 1 @13:24:40.66 Elbow.hold @13:24:40.75 Elbow.reconfigure TightElbow @13:24:40.81 Hand.open @13:24:41.64 Forearm.force -20 @13:24:41.71 Forearm.seek 1 + 350 counts @13:24:42.04 Forearm.reconfigure TightForearm @13:24:42.11 Forearm.seek 1 + 700 counts @13:24:42.52 SE.seek :down @13:24:43.66
Forearm.reconfigure LooseForearm @13:24:43.73 Forearm.seek :retract @13:24:45.36 SE.coast @13:24:45.42
SC.coast @13:24:45.48 Tranferring lysate to PRV @13:24:45.49 CSV.dial :air @13:24:45.88 CS.seek 10ml @13:24:45.97 CSV.dial between air and CTV @13:24:46.33 AIV.dial :bypass @13:24:46.44 Beginning samplePassC @13:24:46.45 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:24:46.56 Hand.open @13:24:46.89 Elbow.seek FlushPuck::Garage @13:24:48.94 Elbow.reconfigure LooseElbow @13:24:49.06 Forearm.seek :garage @13:24:50.09 Hand.close @13:24:51.02 Forearm.seek :retract @13:24:52.02 Elbow.reconfigure TightElbow @13:24:52.09 Elbow.seek CC @13:24:55.07 Elbow.reconfigure LooseElbow @13:24:55.19 Forearm.reconfigure TightForearm @13:24:55.26 Forearm.seek CC @13:24:56.49 CC.seek :closed @13:25:00.80 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.35 @13:25:00.91 Hand.open @13:25:01.72 Forearm.reconfigure LooseForearm @13:25:01.79 Forearm.seek :retract @13:25:02.80 AWV.select :air @13:25:02.82 AIV.dial :bypass @13:25:02.85 PSV.dial PSR @13:25:04.44 Pulling 1.5ml of PSR.flush @13:25:04.45 PSR.select :flush @13:25:04.61 PS.seek 1.5ml @13:25:20.99 delay 5 seconds @13:25:26.01 PSV.dial :waste1 @13:25:26.55 PS.seek 0ml @13:25:43.04 PSV.dial PSR @13:25:43.60 PSR.select :air @13:25:43.74 PS.seek 2.5ml @13:26:10.55 PRV.dial CBV @13:26:10.58
CBV.dial PRV @13:26:11.51 PSV.dial :mixloop @13:26:12.70 CTV.dial :air,avoiding: :intake @13:26:12.71 PTV.dial :PRVmixing @13:26:12.84 PS.seek 0ml @13:26:39.80 PSV.dial :air @13:26:40.34 PS.seek 2.5ml @13:27:07.05 PSV.dial :mixloop @13:27:07.59 PS.seek 0ml @13:27:34.30 PSV.dial :air @13:27:34.84 PS.seek 2.5ml @13:28:01.70 PSV.dial :mixloop @13:28:02.24 PS.seek 0ml @13:28:28.87 Garaging FlushPuck in Collection Clamp @13:28:28.97 Elbow.reconfigure TightElbow @13:28:29.04 Elbow.seek CC @13:28:29.29 Elbow.reconfigure LooseElbow @13:28:29.41 Forearm.seek CC @13:28:30.40 Hand.close @13:28:31.16 CC.seek :open @13:28:35.56 Forearm.seek :retract @13:28:36.58 Elbow.reconfigure TightElbow @13:28:36.65 Elbow.seek :garage @13:28:39.79 Elbow.reconfigure LooseElbow @13:28:39.91 Forearm.reconfigure TightForearm @13:28:39.98 Forearm.seek :garage @13:28:41.17 Hand.open @13:28:41.97 Forearm.reconfigure LooseForearm @13:28:42.04 Forearm.seek :retract @13:28:43.13 Connecting PS to CS to transfer sample @13:28:43.14 CBV.dial AIV @13:28:43.18 AIV.dial :bypass @13:28:43.20 PSV.dial :mixloop @13:28:43.24 PTV.dial :PRVmixing @13:28:43.27 PRV.dial AIV @13:28:43.31 CTV.dial :air,avoiding: :intake @13:28:43.34 Loading lyfil puck from tube 2 into Collection Clamp @13:28:43.60 SC.reconfigure SCtight @13:28:43.67 SC.seek 2 @13:28:45.90 SC.reconfigure SCloose @13:28:46.12 Elbow.reconfigure TightElbow @13:28:46.20 Elbow.seek 2 @13:28:48.74 Forearm.reconfigure TightForearm @13:28:48.82 Forearm.seek 2 + 700 counts @13:28:49.95 SE.reconfigure SEpick @13:28:50.05 SE.seek beamBreak @13:28:53.19 SE.configure SEconfig @13:28:53.36 Elbow.seek 2 @13:28:53.57 Forearm.seek 2 + 300 counts @13:28:53.85 Forearm.reconfigure LooseForearm @13:28:53.92 Hand.close @13:28:54.67 SE.seek :down @13:28:54.90
Forearm.seek :retract @13:28:55.86 Elbow.seek CC @13:28:57.50 SE.coast @13:28:57.64
Elbow.reconfigure LooseElbow @13:28:57.76 Forearm.reconfigure TightForearm @13:28:57.83 Forearm.seek CC @13:28:59.13 CC.seek :closed @13:29:03.43 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.46 @13:29:03.44 Hand.open @13:29:04.21 Forearm.reconfigure LooseForearm @13:29:04.29 Forearm.seek :retract @13:29:05.29 SC.coast @13:29:05.34 CTV.dial CSV,avoiding: :intake @13:29:06.93 CSV.dial CTV @13:29:07.29 CS.seek 5ml @13:29:21.17 delay 1:20 @13:30:41.20 CS.seek 2.5ml @13:30:48.67 delay 40 seconds @13:31:28.71 CS.seek 0ml @13:31:36.17 delay 1:00 @13:32:36.20 PSV.dial :air @13:32:36.73 PS.seek 2.5ml @13:33:03.39 PSV.dial :mixloop @13:33:03.93 delay 3 seconds @13:33:06.95 PSV.dial :air @13:33:07.47 delay 2 seconds @13:33:09.49 PSV.dial :mixloop @13:33:10.01 delay 3 seconds @13:33:13.03 PSV.dial :waste1 @13:33:14.54 PS.seek :empty @13:33:41.14 CBV.dial :waste1 @13:33:41.69 delay 5 seconds @13:33:46.71 CBV.dial AIV @13:33:47.28 delay 10 seconds @13:33:57.31 CBV.dial :waste1 @13:33:57.86 delay 10 seconds @13:34:07.89 CBV.dial AIV @13:34:08.44 delay 10 seconds @13:34:18.47 CTV.dial CSV,avoiding: :intake @13:34:18.48 CBV.dial between AIV and waste1 @13:34:18.91 CSV.dial :air @13:34:19.44 CS.seek 10ml @13:34:45.94 CSV.dial CTV @13:34:46.47 CS.seek 0ml @13:35:13.22 CBV.dial :waste1 @13:35:13.58 delay 10 seconds @13:35:23.61 CTV.dial :air,avoiding: :intake @13:35:25.23 Storing lyfil puck in Collection Clamp to tube 1 @13:35:25.86 SC.reconfigure SCtight @13:35:25.94 SC.seek 1 @13:35:28.16 SC.reconfigure SCloose @13:35:28.39 Elbow.reconfigure TightElbow @13:35:28.46 Elbow.seek 1 @13:35:30.43 Forearm.reconfigure TightForearm @13:35:30.50 Forearm.seek 1 + 700 counts @13:35:31.59 SE.reconfigure SEpick @13:35:31.69 SE.seek beamBreak @13:35:34.82 SE.configure SEconfig @13:35:34.99 Elbow.reconfigure LimpElbow @13:35:35.06 Forearm.reconfigure LooseForearm @13:35:35.14 Forearm.seek 1 @13:35:35.43 Elbow.hold @13:35:35.52 Elbow.reconfigure TightElbow @13:35:35.63 SE.seek 0.03 Pucks below Top Plate @13:35:36.10 Forearm.force -20 @13:35:36.16 Forearm.seek 1 + 350 counts @13:35:36.49 Forearm.reconfigure TightForearm @13:35:36.56 Forearm.seek 1 + 700 counts @13:35:36.85 SE.seek 1.03 Pucks below Top Plate @13:35:37.45 Forearm.reconfigure LooseForearm @13:35:37.52 Forearm.seek :retract @13:35:38.46 Elbow.seek CC @13:35:40.10 Elbow.reconfigure LooseElbow @13:35:40.22 Forearm.seek CC @13:35:41.19 Hand.close @13:35:41.92 CC.seek :open @13:35:46.46 Forearm.seek :retract @13:35:47.48 Elbow.reconfigure TightElbow @13:35:47.55 Elbow.seek 1 @13:35:49.46 Elbow.reconfigure LimpElbow @13:35:49.53 Forearm.seek 1 @13:35:50.56 Elbow.hold @13:35:50.65 Elbow.reconfigure TightElbow @13:35:50.72 Hand.open @13:35:51.66 Forearm.force -20 @13:35:51.73 Forearm.seek 1 + 347 counts @13:35:52.03 Forearm.reconfigure TightForearm @13:35:52.11 Forearm.seek 1 + 700 counts @13:35:52.51 SE.seek :down @13:35:53.64
Forearm.reconfigure LooseForearm @13:35:53.71 Forearm.seek :retract @13:35:55.22 SE.coast @13:35:55.27
SC.coast @13:35:55.33 PSV.dial :air @13:35:56.69 PS.seek 0.499ml @13:36:02.72 delay 3 seconds @13:36:05.74 PSV.dial :mixloop @13:36:06.28 PRV.dial :air @13:36:06.83 delay 3 seconds @13:36:09.85 PRV.dial CBV @13:36:10.39 CBV.dial AIV @13:36:11.38 PRV.dial between CBV and air @13:36:11.75 PRV.dial :air @13:36:12.17 PS.reconfigure PSslow @13:36:12.24 PS.seek 0.599ml @13:36:14.84 PRV.dial :waste2 @13:36:15.69 PS.reconfigure PSconfig @13:36:15.76 PS.seek :empty @13:36:22.69 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:36:22.78 Hand.open @13:36:23.15 Elbow.seek FlushPuck::Garage @13:36:25.17 Elbow.reconfigure LooseElbow @13:36:25.29 Forearm.seek :garage @13:36:26.34 Hand.close @13:36:27.15 Forearm.seek :retract @13:36:28.21 Elbow.reconfigure TightElbow @13:36:28.29 Elbow.seek CC @13:36:31.23 Elbow.reconfigure LooseElbow @13:36:31.35 Forearm.reconfigure TightForearm @13:36:31.42 Forearm.seek CC @13:36:32.63 CC.seek :closed @13:36:36.92 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.37 @13:36:37.03 Hand.open @13:36:37.85 Forearm.reconfigure LooseForearm @13:36:37.92 Forearm.seek :retract @13:36:38.99 Clearing PML for modified lysate @13:36:39.00 CTV.dial :air,avoiding: :intake @13:36:39.04 PRV.dial :waste2 @13:36:39.07 PTV.dial :PRV,avoiding: :puck @13:36:39.94 delay 2 seconds @13:36:41.96 PTV.dial :PRVmixing,avoiding: :puck @13:36:42.83 delay 2 seconds @13:36:44.85 PSV.dial :PML @13:36:44.86 delay 2 seconds @13:36:46.90 PRV.dial :air @13:36:47.76 delay 2 seconds @13:36:49.83 PS.seek 1.499ml @13:37:06.03 delay 5 seconds @13:37:11.06 PS.seek 2.5ml @13:37:22.21 PSV.dial :air @13:37:22.75 PS.seek 0ml @13:37:49.37 mixing lysate with speDil @13:37:49.38 PSV.dial :PML @13:37:49.92 CBV.dial :PRV @13:37:49.93 PRV.dial :air @13:37:49.97 delay 2 seconds @13:37:52.00 PRV.dial :speDil @13:37:52.53 delay 2 seconds @13:37:54.61 PS.reconfigure PSslow1 @13:37:54.69 PS.seek 0.225ml @13:38:18.41 delay 5 seconds @13:38:23.43 PRV.dial CBV @13:38:24.31 delay 2 seconds @13:38:26.38 PS.seek 0.475ml @13:38:52.09 delay 5 seconds @13:38:57.11 PRV.dial :air @13:38:57.66 delay 2 seconds @13:38:59.69 PS.seek 0.965ml @13:39:50.03 delay 10 seconds @13:40:00.06 PRV.dial CBV @13:40:00.61 CTV.dial :air,avoiding: :intake @13:40:00.62 PSV.dial :air @13:40:01.17 PS.reconfigure PSconfig @13:40:01.24 PS.seek 2.5ml @13:40:17.88 delay 3 seconds @13:40:20.90 PSV.dial PTV @13:40:22.30 PTV.dial :PRV,avoiding: :puck @13:40:23.16 PS.seek 0ml @13:40:49.91 PSV.dial :air @13:40:50.77 PS.seek 2.5ml @13:41:17.25 delay 3 seconds @13:41:20.27 PSV.dial PTV @13:41:21.13 PTV.dial :PRV,avoiding: :puck @13:41:21.15 PS.seek 0ml @13:41:47.91 Pushing modified lysate into AIV Injection Loop @13:41:47.92 CBV.dial AIV @13:41:47.96 AIV.dial :bypass @13:41:47.99 PRV.dial CBV @13:41:48.01 PSV.dial :air @13:41:48.88 PS.seek 2.5ml @13:42:15.63 delay 3 seconds @13:42:18.65 PSV.dial :PML @13:42:19.19 PTV.dial :PRVmixing,avoiding: :puck @13:42:20.11 PS.reconfigure PSslow1 @13:42:20.18 PS.seek 1.74ml @13:43:37.60 delay 5 seconds @13:43:42.62 AIV.dial :sample @13:43:43.27 AWV.select :waste @13:43:43.45 PS.reconfigure PSslow2 @13:43:43.53 PS.seek 1.22ml @13:45:30.66 delay 15 seconds @13:45:45.68 AIV.dial :bypass @13:45:46.32 PS.reconfigure PSconfig @13:45:46.39 PS.seek 0ml @13:45:59.74 PSV.dial :air @13:46:00.28 PS.seek 2.5ml @13:46:26.96 PSV.dial :PML @13:46:27.50 PS.seek 0ml @13:46:54.21 AIV.dial :coilToMFB @13:46:54.23 AWV.select :air @13:46:54.43 Extracting Nucleic Acid from AIV @13:46:54.47 SPE.hold 2:46:40,55C @13:46:54.51
Beginning samplePassD @13:46:54.60 ASV.dial :SPEdelivery @13:46:54.71
AWV.select :air @13:46:54.72 AIV.dial :bypass @13:46:54.76 PSV.dial PSR @13:46:55.06 delay 20 seconds @13:46:56.09
Pulling 1.5ml of PSR.killMFB @13:46:56.10 PSR.select :killMFB @13:46:56.27 PS.seek 1.5ml @13:47:13.38 delay 5 seconds @13:47:15.08 primeLysateINT @13:47:15.09 AWV.select :air @13:47:15.13 AIV.dial :coilToMFB @13:47:15.15 ATV.dial AIV @13:47:17.17 AS.reconfigure ASslow1 @13:47:17.24 AS.seek 0.299ml @13:47:18.40
PSV.dial :waste1 @13:47:18.96 PS.seek 0ml @13:47:36.06 PSV.dial PSR @13:47:36.63 PSR.select :air @13:47:36.78 PS.seek 2.5ml @13:48:04.79 PRV.dial CBV @13:48:04.83
CBV.dial PRV @13:48:04.85 PSV.dial :mixloop @13:48:06.11 CTV.dial :air,avoiding: :intake @13:48:06.12 PTV.dial :PRVmixing @13:48:06.18 PS.seek 0ml @13:48:17.94 delay 10 seconds @13:48:27.97 ATV.dial :air2 @13:48:28.85 AS.seek 0.329ml @13:48:34.15
PSV.dial PSR @13:48:35.18 ATV.dial :waste2 @13:48:35.77
Pulling 1.5ml of PSR.flush @13:48:35.78 PSR.select :flush @13:48:35.96 PS.seek 1.5ml @13:48:36.27 AS.reconfigure ASnorm @13:48:36.34 AS.seek :empty @13:48:53.07
delay 5 seconds @13:48:53.39 Lysate primed! @13:48:53.40 AFV.select :holdingCoil @13:48:53.43 ATV.dial :air2 @13:48:54.12 AS.reconfigure ASslow1 @13:48:54.18 AS.seek 0.069ml @13:48:58.09
PSV.dial :waste1 @13:48:58.64 PS.seek 0ml @13:49:08.50 delay 3 seconds @13:49:11.52 ATV.dial :AIV @13:49:12.42 AS.seek 0.469ml @13:49:15.85
PSV.dial PSR @13:49:16.41 PSR.select :air @13:49:16.55 PS.seek 2.5ml @13:49:44.54 PRV.dial CBV @13:49:44.57
CBV.dial PRV @13:49:44.60 PSV.dial :mixloop @13:49:45.85 CTV.dial :air,avoiding: :intake @13:49:45.86 PTV.dial :PRVmixing @13:49:45.93 PS.seek 0ml @13:50:13.78 PSV.dial PSR @13:50:15.07 Pulling 1.5ml of PSR.flush @13:50:15.08 PSR.select :flush @13:50:15.25 PS.seek 1.5ml @13:50:32.19 delay 5 seconds @13:50:33.19 delay 5 seconds @13:50:37.21
PSV.dial :waste1 @13:50:37.77 PS.seek 0ml @13:50:38.25 Solid Phase Extraction Heater at 47.5C @13:50:38.26 ATV.dial ASV @13:50:38.70 ASV.dial :SPEdelivery @13:50:38.80 AS.seek 0.041ml @13:50:54.73
PSV.dial PSR @13:50:55.40 PSR.select :air @13:50:55.54 PS.seek 2.5ml @13:51:22.91 PRV.dial CBV @13:51:22.95
CBV.dial PRV @13:51:22.97 PSV.dial :mixloop @13:51:24.22 CTV.dial :air,avoiding: :intake @13:51:24.23 PTV.dial :PRVmixing @13:51:24.29 PS.seek 0ml @13:51:51.75 PSV.dial PSR @13:51:53.03 Pulling 1.5ml of PSR.flush @13:51:53.04 PSR.select :flush @13:51:53.20 PS.seek 1.5ml @13:52:05.17 delay 10 seconds @13:52:09.85
delay 5 seconds @13:52:14.87 PSV.dial :waste1 @13:52:15.19 rinseLysateLineINT @13:52:15.20 AFV.select :carrierSolution @13:52:15.34 delay 2 seconds @13:52:15.40
PS.seek 0ml @13:52:17.36 AS.reconfigure ASconfig @13:52:17.44 AS.seek 1ml @13:52:31.94
PSV.dial PSR @13:52:32.49 PSR.select :air @13:52:32.63 PS.seek 2.5ml @13:52:59.79 PRV.dial CBV @13:52:59.83
CBV.dial PRV @13:52:59.85 PSV.dial :mixloop @13:53:01.07 CTV.dial :air,avoiding: :intake @13:53:01.08 PTV.dial :PRVmixing @13:53:01.14 PS.reconfigure PSslow @13:53:01.21 PS.seek 1.2ml @13:53:12.86 delay 1 second @13:53:13.88 AFV.select :holdingCoil @13:53:13.98 delay 1 second @13:53:15.00 ATV.dial AIV @13:53:15.67 AIV.dial :coilToMFB @13:53:15.68 AWV.select :waste @13:53:15.83 AS.reconfigure ASslow0 @13:53:15.89 AS.seek 0ml @13:53:28.04
PSV.dial :waste1 @13:53:29.55 PS.reconfigure PSconfig @13:53:29.63 PS.seek 0ml @13:53:43.00 PSV.dial :air @13:53:44.68 PS.seek 2.5ml @13:54:11.69 PSV.dial :mixloop @13:54:12.21 PS.reconfigure PSslow @13:54:12.28 PS.seek 0ml @13:54:56.81 ATV.dial :air2 @13:54:57.50 AS.reconfigure ASslow1 @13:54:57.57 AS.seek 0.05ml @13:55:03.45
PSV.dial :air @13:55:03.98 PS.reconfigure PSconfig @13:55:04.06 PS.seek 2.5ml @13:55:07.91 ATV.dial :AIV @13:55:08.79 AS.seek 0.01ml @13:55:17.17 ATV.dial :waste @13:55:18.83 AS.seek :empty @13:55:21.30 ethanol wash @13:55:21.31 ATV.dial :air2 @13:55:22.71 AS.reconfigure ASslow2 @13:55:22.78 AS.seek 0.009ml @13:55:30.85
PSV.dial :mixloop @13:55:31.41 PS.reconfigure PSslow @13:55:31.48 PS.seek 0ml @13:55:33.12 delay 3 seconds @13:55:36.14 Pulling 0.4ml of Ethanol Wash @13:55:36.15 ATV.dial :ethanol @13:55:37.11 AS.reconfigure ASslow1 @13:55:37.18 AS.seek 0.409ml @13:56:22.54
Cleaning Collection Syringe @13:56:22.55 CSV.dial CSR @13:56:23.13 Pulling 4ml of CSR.flush @13:56:23.14 CSR.select :flush @13:56:23.31 CS.seek 4ml @13:56:34.53 delay 5 seconds @13:56:39.55 CSR.select :air @13:56:39.70 CS.seek 10ml @13:56:56.10 CSV.dial CTV @13:56:56.13
CTV.dial CSV @13:56:57.78 CBV.dial :waste1 @13:56:57.89 delay 3 seconds @13:56:58.34
CS.seek 0ml @13:57:00.91 ATV.dial :air1 @13:57:01.34 delay 3 seconds @13:57:04.42 AS.seek 0.434ml @13:57:09.76 delay 3 seconds @13:57:12.78 ATV.dial :ethanol @13:57:13.40 AS.seek 0.419ml @13:57:16.83 delay 3 seconds @13:57:19.85 ATV.dial ASV @13:57:21.17 AS.seek 0.092ml @13:57:24.80
CSV.dial CSR @13:57:25.40 Pulling 4ml of CSR.flush @13:57:25.41 CSR.select :flush @13:57:25.57 CS.seek 4ml @13:57:36.88 delay 5 seconds @13:57:41.90 CSR.select :air @13:57:42.04 CS.seek 10ml @13:57:58.38 CSV.dial CTV @13:57:58.41
CTV.dial CSV @13:57:58.97 CBV.dial :waste1 @13:57:58.99 CS.seek 0ml @13:58:25.56 Garaging FlushPuck in Collection Clamp @13:58:25.66 Elbow.reconfigure TightElbow @13:58:25.72 Elbow.seek CC @13:58:26.00 Elbow.reconfigure LooseElbow @13:58:26.11 Forearm.seek CC @13:58:27.10 Hand.close @13:58:27.25 delay 3 seconds @13:58:27.82
CC.seek :open @13:58:30.27 ATV.dial :waste2 @13:58:31.16 AS.reconfigure ASconfig @13:58:31.23 AS.seek :empty @13:58:32.21
Forearm.seek :retract @13:58:33.72 Elbow.reconfigure TightElbow @13:58:33.79 Elbow.seek :garage @13:58:36.89 ATV.dial :air2 @13:58:37.04
Elbow.reconfigure LooseElbow @13:58:37.16 Forearm.reconfigure TightForearm @13:58:37.22 Forearm.seek :garage @13:58:37.32 AS.reconfigure ASslow1 @13:58:37.39 AS.seek 1ml @13:58:38.42
Hand.open @13:58:39.25 Forearm.reconfigure LooseForearm @13:58:39.32 Forearm.seek :retract @13:58:40.38 Whole Cell Archive sampling 1500ml @13:58:40.40 Loading wcr puck from tube 2 into Collection Clamp @13:58:40.68 SC.reconfigure SCtight @13:58:40.75 SC.seek 2 @13:58:42.97 SC.reconfigure SCloose @13:58:43.20 Elbow.reconfigure TightElbow @13:58:43.27 Elbow.seek 2 @13:58:45.50 Forearm.reconfigure TightForearm @13:58:45.58 Forearm.seek 2 + 700 counts @13:58:46.78 SE.reconfigure SEpick @13:58:46.88 SE.seek beamBreak @13:58:50.15 SE.configure SEconfig @13:58:50.33 Elbow.seek 2 @13:58:50.54 Forearm.seek 2 + 300 counts @13:58:50.83 Forearm.reconfigure LooseForearm @13:58:50.90 Hand.close @13:58:51.70 Puck::Warning in WCR -- Loaded Last remaining Puck (from Tube 2) @13:58:51.72 SE.seek :down @13:58:51.96 Forearm.seek :retract @13:58:52.96 Elbow.seek CC @13:58:54.66 Elbow.reconfigure LooseElbow @13:58:54.79 SE.coast @13:58:54.83 Forearm.reconfigure TightForearm @13:58:54.94 Forearm.seek CC @13:58:56.19 CC.seek :closed @13:59:00.42 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.39 @13:59:00.43 Hand.open @13:59:01.25 Forearm.reconfigure LooseForearm @13:59:01.32 Forearm.seek :retract @13:59:02.30 SC.coast @13:59:02.36 collectSample 1500ml w/bubble point of 55psi @13:59:02.38 Priming 7ml @13:59:02.40 Gate.power :sampler,:ON @13:59:02.45 Intake.select :close @13:59:02.47 Exhaust.select :close @13:59:02.50 SS.hold @13:59:03.22 Calibrating pressure sensors @13:59:03.23 CTV.dial :air @13:59:04.85 SSV.dial :spit,avoiding: :exhaust @13:59:04.88 SS.reconfigure SSfast @13:59:04.97 SS.seek 5ml @13:59:08.14 SSV.dial CBV @13:59:08.17 CBV.dial SSV @13:59:09.83 delay 3.8 seconds @13:59:13.76 SSV.dial :spit @13:59:15.32 SS.seek 0ml @13:59:18.19 SSV.dial CBV @13:59:19.75 CTV.dial :intake @13:59:20.67 Waiting for external sampler to finish priming... @13:59:20.68 delayUntil "14:04:02.44" @14:01:58.97 delay 5 seconds @14:02:03.99 ATV.dial ASV @14:02:05.02 ASV.dial :SPEdelivery @14:02:05.04 AS.seek 0.02ml @14:04:02.46 SSV.dial CBV @14:04:02.49 CBV.dial SSV @14:04:02.53 Allowing 40s for 7.0ml of remaining 7.0ml @14:04:02.56 Intake.select :open @14:04:04.76 Intake @22.4psia, vacuum from 15.9 to 19.9psi @14:04:04.78 SS.reconfigure SSinhale.with maxSpeed: 150 @14:04:04.87 SS.seek 7ml @14:04:10.40 Stroke completed -- waiting for pressure to equalize ... @14:04:25.73 Intake.select :close @14:04:25.90 SSV.dial :exhaust @14:04:26.79 Exhaust.select :open @14:04:26.93 SS.reconfigure SSexhale @14:04:27.00 SS.seek 0ml @14:04:31.05 Exhaust.select :close @14:04:31.21 Sampling 1500.0ml @14:04:31.24 Intake.select :close @14:04:31.26 Exhaust.select :close @14:04:31.30 SS.reconfigure SSconfig @14:04:31.39 SS.hold @14:04:31.61 Calibrating pressure sensors @14:04:31.63 CTV.dial :air @14:04:32.57 SSV.dial :spit,avoiding: :exhaust @14:04:33.45 SS.reconfigure SSfast @14:04:33.53 SS.seek 5ml @14:04:36.50 SSV.dial CBV @14:04:37.01 CBV.dial SSV @14:04:38.57 delay 3.8 seconds @14:04:42.49 SSV.dial :spit @14:04:44.06 SS.seek 0ml @14:04:46.67 SSV.dial CBV @14:04:48.22 CTV.dial :intake @14:04:49.14 SSV.dial CBV @14:04:49.17 CBV.dial SSV @14:04:49.21 Allowing 130s for 25.0ml of remaining 1500.0ml @14:04:49.24 Intake.select :open @14:04:51.45 Intake @22.5psia, vacuum from 16.0 to 20.0psi @14:04:51.47 SS.reconfigure SSinhale.with maxSpeed: 150 @14:04:51.56 SS.seek 25ml @14:04:59.50 Vacuum too high -- waiting up to 40s @14:05:02.81 Intake @19.1psia, vacuum from 12.6 to 16.6psi @14:05:02.84 SS.reconfigure SSinhale.with maxSpeed: 135 @14:05:02.93 SS.seek 25ml @14:05:08.65 Vacuum too high -- waiting up to 40s @14:05:22.41 delay 5 seconds @14:05:25.18 SS.reconfigure SSinhale.with maxSpeed: 122 @14:05:25.26 SS.seek 25ml @14:05:27.43 ATV.dial :air2 @14:05:28.53 AS.seek 0.52ml @14:05:28.61 Stroke completed -- waiting for pressure to equalize ... @14:05:36.36 Intake.select :close @14:05:36.62 SSV.dial :exhaust @14:05:37.58 Exhaust.select :open @14:05:38.39 SS.reconfigure SSexhale @14:05:38.50 SS.seek 0ml @14:05:48.37 Exhaust.select :close @14:05:48.52 SSV.dial CBV @14:05:48.54 CBV.dial SSV @14:05:49.47 Allowing 130s for 25.0ml of remaining 1475.0ml @14:05:49.49 Intake.select :open @14:05:51.70 Intake @22.1psia, vacuum from 15.6 to 19.6psi @14:05:51.72 SS.reconfigure SSinhale.with maxSpeed: 122 @14:05:51.82 SS.seek 25ml @14:06:02.93 Vacuum too high -- waiting up to 40s @14:06:03.95 Intake @18.9psia, vacuum from 12.4 to 16.4psi @14:06:03.97 SS.reconfigure SSinhale.with maxSpeed: 110 @14:06:04.06 SS.seek 25ml @14:06:08.84 Vacuum too high -- waiting up to 40s @14:06:28.57 Intake @19.4psia, vacuum from 12.9 to 16.9psi @14:06:28.59 SS.reconfigure SSinhale.with maxSpeed: 99 @14:06:28.69 SS.seek 25ml @14:06:30.93 Stroke completed -- waiting for pressure to equalize ... @14:06:35.27 Intake.select :close @14:06:35.45 SSV.dial :exhaust @14:06:36.36 Exhaust.select :open @14:06:36.51 SS.reconfigure SSexhale @14:06:36.58 SS.seek 0ml @14:06:46.18 Exhaust.select :close @14:06:46.32 SSV.dial CBV @14:06:46.35 CBV.dial SSV @14:06:47.28 Allowing 130s for 25.0ml of remaining 1450.0ml @14:06:47.30 Intake.select :open @14:06:49.51 Intake @22.1psia, vacuum from 15.6 to 19.6psi @14:06:49.52 SS.reconfigure SSinhale.with maxSpeed: 99 @14:06:49.62 SS.seek 25ml @14:07:01.15 Vacuum too high -- waiting up to 40s @14:07:09.41 delay 5 seconds @14:07:09.61 Intake @19.2psia, vacuum from 12.7 to 16.7psi @14:07:09.64 SS.reconfigure SSinhale.with maxSpeed: 89 @14:07:09.73 SS.seek 25ml @14:07:14.44 ATV.dial ASV @14:07:15.49 ASV.dial :wasteR @14:07:15.89 AS.seek 0.3ml @14:07:18.88 Stroke completed -- waiting for pressure to equalize ... @14:07:33.50 Intake.select :close @14:07:33.68 SSV.dial :exhaust @14:07:34.59 Exhaust.select :open @14:07:34.73 SS.reconfigure SSexhale @14:07:34.80 SS.seek 0ml @14:07:44.48 Exhaust.select :close @14:07:44.61 SSV.dial CBV @14:07:44.64 CBV.dial SSV @14:07:45.56 Allowing 130s for 25.0ml of remaining 1425.0ml @14:07:45.58 Intake.select :open @14:07:47.79 Intake @22.2psia, vacuum from 15.7 to 19.7psi @14:07:47.81 SS.reconfigure SSinhale.with maxSpeed: 89 @14:07:47.91 SS.seek 25ml @14:07:57.53 Vacuum too high -- waiting up to 40s @14:08:00.48 delay 20 seconds @14:08:02.52 Intake @19.4psia, vacuum from 12.9 to 16.9psi @14:08:02.55 SS.reconfigure SSinhale.with maxSpeed: 80 @14:08:02.65 SS.seek 25ml @14:08:08.12 Vacuum too high -- waiting up to 40s @14:08:15.43 Intake @19.6psia, vacuum from 13.1 to 17.1psi @14:08:15.46 SS.reconfigure SSinhale.with maxSpeed: 72 @14:08:15.55 SS.seek 25ml @14:08:20.52 AS.seek 0.07ml @14:08:24.82 Stroke completed -- waiting for pressure to equalize ... @14:08:34.05 Intake.select :close @14:08:34.23 SSV.dial :exhaust @14:08:35.14 Exhaust.select :open @14:08:35.28 SS.reconfigure SSexhale @14:08:35.35 SS.seek 0ml @14:08:45.08 Exhaust.select :close @14:08:45.19 SSV.dial CBV @14:08:45.22 CBV.dial SSV @14:08:46.14 Allowing 130s for 25.0ml of remaining 1400.0ml @14:08:46.15 Intake.select :open @14:08:48.37 Intake @28.2psia, vacuum from 20.0 to 24.0psi @14:08:48.39 SS.reconfigure SSinhale.with maxSpeed: 72 @14:08:48.48 SS.seek 25ml @14:08:56.03 Vacuum too high -- waiting up to 40s @14:08:57.02 Intake @24.9psia, vacuum from 18.4 to 22.4psi @14:08:57.04 SS.reconfigure SSinhale.with maxSpeed: 65 @14:08:57.13 SS.seek 25ml @14:09:07.06 delay 20 seconds @14:09:17.21 Stroke completed -- waiting for pressure to equalize ... @14:09:24.00 Intake.select :close @14:09:24.16 SSV.dial :exhaust @14:09:25.06 Exhaust.select :open @14:09:25.20 SS.reconfigure SSexhale @14:09:25.27 SS.seek 0ml @14:09:27.08 ATV.dial :waste2 @14:09:27.89 AS.reconfigure ASconfig @14:09:27.97 AS.seek :empty @14:09:32.86 Elution @14:09:32.88 ATV.dial :air1 @14:09:34.06 AS.reconfigure ASslow1 @14:09:34.13 AS.seek 0.049ml @14:09:35.00 Exhaust.select :close @14:09:35.14 SSV.dial CBV @14:09:35.17 CBV.dial SSV @14:09:36.09 Allowing 130s for 25.0ml of remaining 1375.0ml @14:09:36.11 Intake.select :open @14:09:38.32 Intake @28.3psia, vacuum from 20.0 to 24.0psi @14:09:38.34 SS.reconfigure SSinhale.with maxSpeed: 65 @14:09:38.43 SS.seek 25ml @14:09:44.52 delay 3 seconds @14:09:47.54 ATV.dial :ElutionWater @14:09:48.25 AS.seek 0.109ml @14:09:49.88 Vacuum too high -- waiting up to 40s @14:09:50.64 Intake @24.9psia, vacuum from 18.4 to 22.4psi @14:09:50.67 SS.reconfigure SSinhale.with maxSpeed: 59 @14:09:50.76 SS.seek 25ml @14:10:00.61 delay 3 seconds @14:10:03.63 ATV.dial ASV,via: :air2 @14:10:05.84 ASV.dial :SPEelution @14:10:06.49 AFV.select :carrierSolution @14:10:06.65 AS.reconfigure ASnorm @14:10:06.72 AS.seek 0.759ml @14:10:09.19 Stroke completed -- waiting for pressure to equalize ... @14:10:15.37 Intake.select :close @14:10:15.53 SSV.dial :exhaust @14:10:16.45 Exhaust.select :open @14:10:16.59 SS.reconfigure SSexhale @14:10:16.66 SS.seek 0ml @14:10:26.46 Exhaust.select :close @14:10:26.58 SSV.dial CBV @14:10:26.61 CBV.dial SSV @14:10:27.53 Allowing 130s for 25.0ml of remaining 1350.0ml @14:10:27.55 Intake.select :open @14:10:29.81 Intake @28.5psia, vacuum from 20.0 to 24.0psi @14:10:29.83 SS.reconfigure SSinhale.with maxSpeed: 59 @14:10:29.92 SS.seek 25ml @14:10:39.46 Vacuum too high -- waiting up to 40s @14:10:39.74 delay 3 seconds @14:10:40.42 Intake @25.0psia, vacuum from 18.5 to 22.5psi @14:10:40.44 SS.reconfigure SSinhale.with maxSpeed: 53 @14:10:40.54 SS.seek 25ml @14:10:42.77 AFV.select :holdingCoil @14:10:42.94 AS.reconfigure ASslow1 @14:10:43.00 AS.seek 0.474ml @14:11:03.99 Stroke completed -- waiting for pressure to equalize ... @14:11:08.34 Intake.select :close @14:11:08.50 SSV.dial :exhaust @14:11:09.41 Exhaust.select :open @14:11:10.01 SS.reconfigure SSexhale @14:11:10.10 SS.seek 0ml @14:11:19.95 Exhaust.select :close @14:11:20.09 SSV.dial CBV @14:11:20.11 CBV.dial SSV @14:11:21.03 Allowing 130s for 25.0ml of remaining 1325.0ml @14:11:21.05 Intake.select :open @14:11:23.27 Intake @28.7psia, vacuum from 20.0 to 24.0psi @14:11:23.29 SS.reconfigure SSinhale.with maxSpeed: 53 @14:11:23.38 SS.seek 25ml @14:11:24.49 Session Ended @14:11:34.78 Vacuum too high -- waiting up to 40s @14:11:35.32 Intake @25.7psia, vacuum from 19.2 to 23.2psi @14:11:35.34 SS.reconfigure SSinhale.with maxSpeed: 48 @14:11:35.42 SS.seek 25ml @14:11:40.63 delay 5 seconds @14:11:45.71 AS.reconfigure ASslow2 @14:11:45.77 AS.seek 0.439ml @14:12:00.18 Stroke completed -- waiting for pressure to equalize ... @14:12:03.91 Intake.select :close @14:12:04.07 SSV.dial :exhaust @14:12:04.97 Exhaust.select :open @14:12:05.10 SS.reconfigure SSexhale @14:12:05.16 SS.seek 0ml @14:12:14.80 Exhaust.select :close @14:12:14.93 SSV.dial CBV @14:12:14.96 CBV.dial SSV @14:12:15.86 Allowing 130s for 25.0ml of remaining 1300.0ml @14:12:15.88 Intake.select :open @14:12:18.06 Intake @28.7psia, vacuum from 20.0 to 24.0psi @14:12:18.09 SS.reconfigure SSinhale.with maxSpeed: 48 @14:12:18.16 SS.seek 25ml @14:12:21.30 delay 1:00 @14:12:55.46 Stroke completed -- waiting for pressure to equalize ... @14:12:59.19 Intake.select :close @14:12:59.35 SSV.dial :exhaust @14:13:00.25 Exhaust.select :open @14:13:00.38 SS.reconfigure SSexhale @14:13:00.44 SS.seek 0ml @14:13:09.94 Exhaust.select :close @14:13:10.07 SSV.dial CBV @14:13:10.10 CBV.dial SSV @14:13:11.00 Allowing 130s for 25.0ml of remaining 1275.0ml @14:13:11.01 Intake.select :open @14:13:13.21 Intake @28.7psia, vacuum from 20.0 to 24.0psi @14:13:13.22 SS.reconfigure SSinhale.with maxSpeed: 48 @14:13:13.30 SS.seek 25ml @14:13:21.32 pushing eluate to holding coil @14:13:21.40 AS.seek 0.279ml @14:13:43.46 Vacuum too high -- waiting up to 40s @14:13:44.22 Intake @25.7psia, vacuum from 19.2 to 23.2psi @14:13:44.24 SS.reconfigure SSinhale.with maxSpeed: 43 @14:13:44.32 SS.seek 25ml @14:13:52.94 Stroke completed -- waiting for pressure to equalize ... @14:13:56.06 Intake.select :close @14:13:56.69 SSV.dial :exhaust @14:13:57.59 Exhaust.select :open @14:13:57.72 SS.reconfigure SSexhale @14:13:57.78 SS.seek 0ml @14:14:07.59 Exhaust.select :close @14:14:07.72 SSV.dial CBV @14:14:07.74 CBV.dial SSV @14:14:08.64 Allowing 130s for 25.0ml of remaining 1250.0ml @14:14:08.66 Intake.select :open @14:14:10.85 Intake @28.8psia, vacuum from 20.0 to 24.0psi @14:14:10.86 SS.reconfigure SSinhale.with maxSpeed: 43 @14:14:10.95 SS.seek 25ml @14:14:52.11 Stroke completed -- waiting for pressure to equalize ... @14:14:55.23 Intake.select :close @14:14:55.39 SSV.dial :exhaust @14:14:56.28 Exhaust.select :open @14:14:56.41 SS.reconfigure SSexhale @14:14:56.47 SS.seek 0ml @14:15:06.19 Exhaust.select :close @14:15:06.31 SSV.dial CBV @14:15:06.34 CBV.dial SSV @14:15:07.25 Allowing 130s for 25.0ml of remaining 1225.0ml @14:15:07.27 Intake.select :open @14:15:09.44 Intake @28.7psia, vacuum from 20.0 to 24.0psi @14:15:09.46 SS.reconfigure SSinhale.with maxSpeed: 43 @14:15:09.54 SS.seek 25ml @14:15:50.74 Stroke completed -- waiting for pressure to equalize ... @14:15:53.86 Intake.select :close @14:15:54.02 SSV.dial :exhaust @14:15:54.92 Exhaust.select :open @14:15:55.05 SS.reconfigure SSexhale @14:15:55.11 SS.seek 0ml @14:16:02.83 delay 30 seconds @14:16:04.76 Exhaust.select :close @14:16:04.88 SSV.dial CBV @14:16:04.91 CBV.dial SSV @14:16:05.81 Allowing 130s for 25.0ml of remaining 1200.0ml @14:16:05.82 Intake.select :open @14:16:08.02 Intake @28.7psia, vacuum from 20.0 to 24.0psi @14:16:08.03 SS.reconfigure SSinhale.with maxSpeed: 43 @14:16:08.11 SS.seek 25ml @14:16:32.85 ATV.dial :waste,via: :air3 @14:16:34.63 AS.reconfigure ASconfig @14:16:34.69 AS.seek :empty @14:16:49.58 Stroke completed -- waiting for pressure to equalize ... @14:16:51.10 Parking Bleach in LysateSource line and SPEcolumn @14:16:51.12 ATV.dial :air3 @14:16:51.98 AS.reconfigure ASslow1 @14:16:52.05 AS.seek 0.029ml @14:16:52.88 Intake.select :close @14:16:53.04 SSV.dial :exhaust @14:16:53.92 Exhaust.select :open @14:16:54.05 SS.reconfigure SSexhale @14:16:54.11 SS.seek 0ml @14:16:58.34 ATV.dial :ElutionWater @14:16:58.97 AS.seek 0.059ml @14:17:03.78 Exhaust.select :close @14:17:03.91 SSV.dial CBV @14:17:03.94 CBV.dial SSV @14:17:04.84 Allowing 130s for 25.0ml of remaining 1175.0ml @14:17:04.85 Intake.select :open @14:17:05.42 ATV.dial :air3 @14:17:06.05 AS.seek 0.069ml @14:17:07.54 Intake @28.8psia, vacuum from 20.0 to 24.0psi @14:17:07.56 SS.reconfigure SSinhale.with maxSpeed: 43 @14:17:07.64 SS.seek 25ml @14:17:08.35 delay 2 seconds @14:17:10.37 ATV.dial :bleach @14:17:10.83 AS.seek 0.769ml @14:17:46.21 Vacuum too high -- waiting up to 40s @14:17:46.97 Intake @26.0psia, vacuum from 19.5 to 23.5psi @14:17:46.99 SS.reconfigure SSinhale.with maxSpeed: 39 @14:17:47.07 SS.seek 25ml @14:17:50.69 Stroke completed -- waiting for pressure to equalize ... @14:17:53.21 Intake.select :close @14:17:53.37 SSV.dial :exhaust @14:17:54.25 Exhaust.select :open @14:17:54.38 SS.reconfigure SSexhale @14:17:54.44 SS.seek 0ml @14:18:03.93 Exhaust.select :close @14:18:04.05 SSV.dial CBV @14:18:04.08 CBV.dial SSV @14:18:04.98 Allowing 130s for 25.0ml of remaining 1150.0ml @14:18:05.00 Intake.select :open @14:18:07.19 Intake @28.9psia, vacuum from 20.0 to 24.0psi @14:18:07.20 SS.reconfigure SSinhale.with maxSpeed: 39 @14:18:07.28 SS.seek 25ml @14:18:52.65 Stroke completed -- waiting for pressure to equalize ... @14:18:55.77 Intake.select :close @14:18:55.93 SSV.dial :exhaust @14:18:56.81 Exhaust.select :open @14:18:56.94 SS.reconfigure SSexhale @14:18:57.00 SS.seek 0ml @14:19:06.60 Exhaust.select :close @14:19:06.73 SSV.dial CBV @14:19:06.76 CBV.dial SSV @14:19:07.66 Allowing 130s for 25.0ml of remaining 1125.0ml @14:19:07.67 Intake.select :open @14:19:09.86 Intake @28.8psia, vacuum from 20.0 to 24.0psi @14:19:09.89 SS.reconfigure SSinhale.with maxSpeed: 39 @14:19:09.96 SS.seek 25ml @14:19:31.86 delay 3 seconds @14:19:34.89 ATV.dial :air3 @14:19:35.46 delay 3 seconds @14:19:38.53 AS.seek 0.794ml @14:19:43.83 delay 3 seconds @14:19:46.85 ATV.dial :bleach @14:19:47.49 AS.seek 0.779ml @14:19:50.77 delay 3 seconds @14:19:53.79 ATV.dial :AIV @14:19:55.10 AS.reconfigure ASconfig @14:19:55.16 AS.seek 0.015ml @14:19:55.36 Stroke completed -- waiting for pressure to equalize ... @14:19:59.10 Intake.select :close @14:19:59.26 SSV.dial :exhaust @14:20:00.16 Exhaust.select :open @14:20:00.29 SS.reconfigure SSexhale @14:20:00.35 SS.seek 0ml @14:20:09.99 Exhaust.select :close @14:20:10.13 SSV.dial CBV @14:20:10.15 CBV.dial SSV @14:20:11.06 Allowing 130s for 25.0ml of remaining 1100.0ml @14:20:11.08 Intake.select :open @14:20:13.27 Intake @28.7psia, vacuum from 20.0 to 24.0psi @14:20:13.29 SS.reconfigure SSinhale.with maxSpeed: 39 @14:20:13.37 SS.seek 25ml @14:20:39.40 ATV.dial :waste @14:20:40.95 AS.seek :empty @14:20:42.21 ATV.dial :air3 @14:20:42.91 AS.reconfigure ASslow1 @14:20:42.97 AS.seek 0.009ml @14:20:45.32 delay 2 seconds @14:20:47.34 ATV.dial :bleach @14:20:47.46 Vacuum too high -- waiting up to 40s @14:20:47.81 AS.seek 0.579ml @14:20:48.03 Intake @26.0psia, vacuum from 19.5 to 23.5psi @14:20:48.05 SS.reconfigure SSinhale.with maxSpeed: 35 @14:20:48.12 SS.seek 25ml @14:21:02.08 Stroke completed -- waiting for pressure to equalize ... @14:21:05.20 Intake.select :close @14:21:05.36 SSV.dial :exhaust @14:21:06.26 Exhaust.select :open @14:21:06.39 SS.reconfigure SSexhale @14:21:06.45 SS.seek 0ml @14:21:15.91 Exhaust.select :close @14:21:16.03 SSV.dial CBV @14:21:16.06 CBV.dial SSV @14:21:16.97 Allowing 130s for 25.0ml of remaining 1075.0ml @14:21:16.99 Intake.select :open @14:21:19.16 Intake @28.8psia, vacuum from 20.0 to 24.0psi @14:21:19.18 SS.reconfigure SSinhale.with maxSpeed: 35 @14:21:19.26 SS.seek 25ml @14:22:10.16 Stroke completed -- waiting for pressure to equalize ... @14:22:13.28 Intake.select :close @14:22:13.44 SSV.dial :exhaust @14:22:14.34 Exhaust.select :open @14:22:14.47 SS.reconfigure SSexhale @14:22:14.53 SS.seek 0ml @14:22:23.95 Exhaust.select :close @14:22:24.06 SSV.dial CBV @14:22:24.09 CBV.dial SSV @14:22:24.98 Allowing 130s for 25.0ml of remaining 1050.0ml @14:22:24.99 Intake.select :open @14:22:27.18 Intake @28.7psia, vacuum from 20.0 to 24.0psi @14:22:27.21 SS.reconfigure SSinhale.with maxSpeed: 35 @14:22:27.28 SS.seek 25ml @14:22:42.82 delay 3 seconds @14:22:45.84 ATV.dial :air3 @14:22:46.43 delay 3 seconds @14:22:49.50 AS.seek 0.604ml @14:22:54.88 delay 3 seconds @14:22:57.91 ATV.dial :bleach @14:22:58.55 AS.seek 0.589ml @14:23:01.88 delay 3 seconds @14:23:04.90 ATV.dial :ASV @14:23:05.97 ASV.dial :wasteR @14:23:06.60 AS.seek 0ml @14:23:18.13 Stroke completed -- waiting for pressure to equalize ... @14:23:21.11 Intake.select :close @14:23:21.29 SSV.dial :exhaust @14:23:22.19 Exhaust.select :open @14:23:22.32 SS.reconfigure SSexhale @14:23:22.38 SS.seek 0ml @14:23:31.98 Exhaust.select :close @14:23:32.10 SSV.dial CBV @14:23:32.13 CBV.dial SSV @14:23:33.03 Allowing 130s for 25.0ml of remaining 1025.0ml @14:23:33.05 Intake.select :open @14:23:35.24 Intake @28.8psia, vacuum from 20.0 to 24.0psi @14:23:35.26 SS.reconfigure SSinhale.with maxSpeed: 35 @14:23:35.34 SS.seek 25ml @14:24:25.77 Stroke completed -- waiting for pressure to equalize ... @14:24:28.89 Intake.select :close @14:24:29.05 SSV.dial :exhaust @14:24:29.95 Exhaust.select :open @14:24:30.08 SS.reconfigure SSexhale @14:24:30.14 SS.seek 0ml @14:24:39.71 Exhaust.select :close @14:24:39.83 SSV.dial CBV @14:24:39.86 CBV.dial SSV @14:24:40.76 Allowing 130s for 25.0ml of remaining 1000.0ml @14:24:40.78 Intake.select :open @14:24:42.96 Intake @28.7psia, vacuum from 20.0 to 24.0psi @14:24:42.97 SS.reconfigure SSinhale.with maxSpeed: 35 @14:24:43.05 SS.seek 25ml @14:25:05.40 delay 1:00 @14:25:33.43 Stroke completed -- waiting for pressure to equalize ... @14:25:37.16 Intake.select :close @14:25:37.32 SSV.dial :exhaust @14:25:38.22 Exhaust.select :open @14:25:38.35 SS.reconfigure SSexhale @14:25:38.41 SS.seek 0ml @14:25:47.86 Exhaust.select :close @14:25:47.99 SSV.dial CBV @14:25:48.02 CBV.dial SSV @14:25:48.92 Allowing 130s for 25.0ml of remaining 975.0ml @14:25:48.94 Intake.select :open @14:25:51.13 Intake @28.6psia, vacuum from 20.0 to 24.0psi @14:25:51.14 SS.reconfigure SSinhale.with maxSpeed: 35 @14:25:51.22 SS.seek 25ml @14:26:05.42 AFV.select :carrierSolution @14:26:05.53 delay 2 seconds @14:26:07.55 AS.reconfigure ASconfig @14:26:07.61 AS.seek 1ml @14:26:41.63 Stroke completed -- waiting for pressure to equalize ... @14:26:45.97 Intake.select :close @14:26:46.13 SSV.dial :exhaust @14:26:47.03 Exhaust.select :open @14:26:47.16 SS.reconfigure SSexhale @14:26:47.22 SS.seek 0ml @14:26:56.66 Exhaust.select :close @14:26:56.79 SSV.dial CBV @14:26:56.82 CBV.dial SSV @14:26:57.72 Allowing 130s for 25.0ml of remaining 950.0ml @14:26:57.73 Intake.select :open @14:26:59.92 Intake @28.8psia, vacuum from 20.0 to 24.0psi @14:26:59.94 SS.reconfigure SSinhale.with maxSpeed: 35 @14:27:00.49 SS.seek 25ml @14:27:05.40 delay 1 second @14:27:06.42 AFV.select :holdingCoil @14:27:06.52 delay 1 second @14:27:07.54 ATV.dial ASV @14:27:07.55 ASV.dial :wasteR @14:27:07.58 AS.reconfigure ASslow1 @14:27:07.65 AS.seek 0ml @14:27:50.97 Stroke completed -- waiting for pressure to equalize ... @14:27:55.31 Intake.select :close @14:27:55.47 SSV.dial :exhaust @14:27:56.38 Exhaust.select :open @14:27:56.51 SS.reconfigure SSexhale @14:27:56.57 SS.seek 0ml @14:28:06.01 Exhaust.select :close @14:28:06.12 SSV.dial CBV @14:28:06.17 CBV.dial SSV @14:28:07.03 Allowing 130s for 25.0ml of remaining 925.0ml @14:28:07.05 Intake.select :open @14:28:09.22 Intake @28.9psia, vacuum from 20.0 to 24.0psi @14:28:09.24 SS.reconfigure SSinhale.with maxSpeed: 35 @14:28:09.32 SS.seek 25ml @14:28:35.31 Vacuum too high -- waiting up to 40s @14:28:35.84 Intake @26.6psia, vacuum from 20.0 to 24.0psi @14:28:35.86 SS.reconfigure SSinhale.with maxSpeed: 32 @14:28:35.94 SS.seek 25ml @14:29:03.25 Stroke completed -- waiting for pressure to equalize ... @14:29:09.43 Intake.select :close @14:29:09.59 SSV.dial :exhaust @14:29:10.48 Exhaust.select :open @14:29:10.61 SS.reconfigure SSexhale @14:29:10.67 SS.seek 0ml @14:29:19.77 Exhaust.select :close @14:29:19.89 SSV.dial CBV @14:29:19.92 CBV.dial SSV @14:29:20.83 Allowing 130s for 25.0ml of remaining 900.0ml @14:29:20.84 Intake.select :open @14:29:23.03 Intake @29.1psia, vacuum from 20.0 to 24.0psi @14:29:23.06 SS.reconfigure SSinhale.with maxSpeed: 32 @14:29:23.13 SS.seek 25ml @14:30:19.02 Stroke completed -- waiting for pressure to equalize ... @14:30:29.07 delay 20 seconds @14:30:49.09 ATV.dial :air3 @14:30:50.71 AS.seek 0.59ml @14:30:59.38 SS.reconfigure SSconfig @14:30:59.45 SS.hold @14:30:59.58 Intake.select :close @14:30:59.74 ShallowSampler::Clogged in WCR -- Intake @29psia & Outlet @25psia won't equalize after 625.0ml @14:30:59.75 SSV.dial :exhaust @14:31:00.65 Exhaust.select :open @14:31:01.29 SS.reconfigure SSexhale @14:31:01.35 SS.seek 0ml @14:31:10.81 Exhaust.select :close @14:31:10.93 Sampled 625.0ml @14:31:10.95 Drying puck before fixing for 4:00 @14:31:10.97 CBV.dial :waste2b @14:31:11.86 delay 1:00 @14:31:13.94 Gate.power :sampler,:OFF @14:32:11.89 CSV.dial :air @14:32:12.51 CS.reconfigure CSfast @14:32:12.58 CS.seek 10ml @14:32:39.19 Pumping wcr puck from tube 2 up to 55psi... @14:32:39.20 CBV.dial SSV @14:32:40.65 CBV.dial between CSV and waste2b @14:32:41.76 CSV.dial between air and CTV @14:32:42.12 CS.seek 7.35ml @14:32:49.66 delay 3 seconds @14:32:49.84 CTV.dial CSV,avoiding: :air @14:32:49.87 CSV.dial CTV @14:32:50.80 CS.reconfigure CSpressurize @14:32:50.87 CS.seek 0ml @14:32:52.68 ATV.dial :ASV @14:32:54.10 ASV.dial :wasteR @14:32:54.19 AS.seek 0.09ml @14:33:07.60 CBV.dial :waste2b @14:33:08.00 CS.reconfigure CSconfig @14:33:08.07 CS.seek :empty @14:33:11.84 delay 1:00 @14:34:11.87 CTV.dial :air,avoiding: :intake @14:34:13.42 Fix #1 of 2 (with rnal) for 2:00 @14:34:13.44 CSV.dial CSR @14:34:14.02 Pulling 2ml of CSR.rnal @14:34:14.04 CSR.select :rnal @14:34:14.18 CS.seek 2ml @14:34:20.28 delay 5 seconds @14:34:25.30 CSR.select :air @14:34:25.44 CS.reconfigure CSfast @14:34:25.50 CS.seek 10ml @14:34:35.02 delay 30 seconds @14:34:46.88 Pumping wcr puck from tube 2 up to 55psi... @14:34:46.89 CBV.dial SSV @14:34:48.34 CBV.dial between CSV and waste2b @14:34:49.45 CSV.dial between air and CTV @14:34:50.15 CS.seek 7.337ml @14:34:57.88 CTV.dial CSV,avoiding: :air @14:34:57.91 CSV.dial CTV @14:34:59.50 CS.reconfigure CSpressurize @14:34:59.57 CS.seek 0ml @14:35:05.04 ATV.dial :extSrc @14:35:05.82 delay 3 seconds @14:35:08.84 ATV.dial :ElutionWater @14:35:09.96 AS.seek 0.12ml @14:35:16.29 ATV.dial :air3 @14:35:16.93 AS.seek 0.13ml @14:35:18.49 delay 2:00 @14:35:19.27 delay 2 seconds @14:35:21.29 ATV.dial :bleach @14:35:21.77 AS.seek 0.23ml @14:35:42.23 delay 3 seconds @14:35:45.25 ATV.dial :air3 @14:35:45.80 delay 3 seconds @14:35:48.87 AS.seek 0.255ml @14:35:54.19 delay 3 seconds @14:35:57.66 ATV.dial :bleach @14:35:58.29 AS.seek 0.24ml @14:36:01.60 delay 3 seconds @14:36:04.62 ATV.dial :ASV @14:36:05.66 ASV.dial :SPEdelivery @14:36:06.05 AFV.select :carrierSolution @14:36:06.16 delay 2 seconds @14:36:08.18 AS.reconfigure ASconfig @14:36:08.24 AS.seek 0.5ml @14:36:23.49 delay 1 second @14:36:24.51 AFV.select :holdingCoil @14:36:24.66 AS.reconfigure ASslow1 @14:36:24.72 AS.seek 0.17ml @14:37:18.51 CBV.dial :waste2b @14:37:18.89 CS.reconfigure CSconfig @14:37:18.96 CS.seek :empty @14:37:20.75 delay 1:00 @14:37:31.39 SPE.hold 2:46:40,95C @14:37:31.44 delay 3:00 @14:38:20.78 CTV.dial :air,avoiding: :intake @14:38:22.41 Fix #2 of 2 (with rnal) for 2:00 @14:38:22.43 CSV.dial CSR @14:38:23.02 Pulling 2ml of CSR.rnal @14:38:23.04 CSR.select :rnal @14:38:23.18 CS.seek 2ml @14:38:29.63 delay 5 seconds @14:38:34.65 CSR.select :air @14:38:34.79 CS.reconfigure CSfast @14:38:34.85 CS.seek 10ml @14:38:57.28 Pumping wcr puck from tube 2 up to 55psi... @14:38:57.29 CBV.dial SSV @14:38:58.77 CBV.dial between CSV and waste2b @14:38:59.92 CSV.dial between air and CTV @14:39:00.65 CS.seek 7.364ml @14:39:08.66 CTV.dial CSV,avoiding: :air @14:39:08.69 CSV.dial CTV @14:39:10.36 CS.reconfigure CSpressurize @14:39:10.43 CS.seek 0ml @14:39:29.53 delay 2:00 @14:40:31.49 AFV.select :carrierSolution @14:40:31.60 delay 2 seconds @14:40:33.62 AS.reconfigure ASconfig @14:40:33.68 AS.seek 1ml @14:41:21.68 delay 1 second @14:41:22.70 AFV.select :holdingCoil @14:41:22.80 delay 1 second @14:41:23.82 ASV.dial :wasteD @14:41:24.91 AS.seek 0ml @14:41:29.55 CBV.dial :waste2b @14:41:29.93 CS.reconfigure CSconfig @14:41:30.00 CS.seek :empty @14:41:32.26 delay 1:00 @14:42:22.71 Priming Eluate @14:42:22.72 AFV.select :carrierSolution @14:42:22.84 delay 2 seconds @14:42:24.87 AS.seek 0.5ml @14:42:32.28 CTV.dial :air,avoiding: :intake @14:42:33.94 Storing wcr puck in Collection Clamp to tube 1 @14:42:34.12 SC.reconfigure SCtight @14:42:34.19 SC.seek 1 @14:42:36.35 SC.reconfigure SCloose @14:42:36.56 Elbow.reconfigure TightElbow @14:42:36.63 Elbow.seek 1 @14:42:38.55 Forearm.reconfigure TightForearm @14:42:38.62 Forearm.seek 1 + 700 counts @14:42:39.64 SE.reconfigure SEpick @14:42:39.74 SE.seek beamBreak @14:42:43.46 SE.configure SEconfig @14:42:43.65 Elbow.reconfigure LimpElbow @14:42:43.72 Forearm.reconfigure LooseForearm @14:42:43.78 Forearm.seek 1 @14:42:44.09 Elbow.hold @14:42:44.18 Elbow.reconfigure TightElbow @14:42:44.29 SE.seek 1.03 Pucks below Top Plate @14:42:44.73 Forearm.force -20 @14:42:44.79 Forearm.seek 1 + 328 counts @14:42:45.12 Forearm.reconfigure TightForearm @14:42:45.19 Forearm.seek 1 + 700 counts @14:42:45.54 SE.seek 2.03 Pucks below Top Plate @14:42:46.16 Forearm.reconfigure LooseForearm @14:42:46.23 Forearm.seek :retract @14:42:47.19 Elbow.seek CC @14:42:48.92 Elbow.reconfigure LooseElbow @14:42:49.04 Forearm.seek CC @14:42:50.06 Hand.close @14:42:50.86 CC.seek :open @14:42:53.91 delay 1 second @14:42:54.93 AFV.select :holdingCoil @14:42:55.03 ATV.dial ASV @14:42:55.04 ASV.dial :SPErecovery @14:42:55.35 Forearm.seek :retract @14:42:55.70 Swish #1 of 2 @14:42:55.78 AS.reconfigure ASslow0 @14:42:55.84 AS.seek 0.6ml @14:42:56.36 Elbow.reconfigure TightElbow @14:42:56.43 Elbow.seek 1 @14:42:58.33 Elbow.reconfigure LimpElbow @14:42:58.41 Forearm.seek 1 @14:42:59.45 Elbow.hold @14:42:59.54 Elbow.reconfigure TightElbow @14:42:59.60 Hand.open @14:43:00.47 Forearm.force -20 @14:43:00.53 Forearm.seek 1 + 344 counts @14:43:00.85 Forearm.reconfigure TightForearm @14:43:00.92 Forearm.seek 1 + 700 counts @14:43:01.23 SE.seek :down @14:43:02.35 Forearm.reconfigure LooseForearm @14:43:02.42 Forearm.seek :retract @14:43:03.87 SE.coast @14:43:03.92 SC.coast @14:43:03.98 Starting finalPurge @14:43:03.99 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:43:04.09 Hand.open @14:43:04.49 Elbow.seek FlushPuck::Garage @14:43:06.37 AS.seek 0.5ml @14:43:06.69
Elbow.reconfigure LooseElbow @14:43:06.81 Forearm.seek :garage @14:43:07.86 Hand.close @14:43:08.73 Forearm.seek :retract @14:43:09.78 Elbow.reconfigure TightElbow @14:43:09.84 Elbow.seek CC @14:43:12.83 Elbow.reconfigure LooseElbow @14:43:12.94 Forearm.reconfigure TightForearm @14:43:13.00 Forearm.seek CC @14:43:14.27 CC.seek :closed @14:43:16.73 Swish #2 of 2 @14:43:16.84 AS.seek 0.6ml @14:43:18.53
Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.37 @14:43:18.61 Hand.open @14:43:19.42 Forearm.reconfigure LooseForearm @14:43:19.48 Forearm.seek :retract @14:43:20.49 Flushing the Collection Valve::Manifold @14:43:20.63 CSV.dial CSR @14:43:21.20 Pulling 5ml of CSR.flush @14:43:21.21 CSR.select :flush @14:43:21.36 CS.seek 5ml @14:43:27.37 AS.seek 0.5ml @14:43:35.70
delay 5 seconds @14:43:37.76 delay 3 seconds @14:43:40.72
CSR.select :air @14:43:40.85 AS.reconfigure ASslow1 @14:43:40.91 AS.seek 0.684ml @14:43:41.02
CS.reconfigure CSfast @14:43:41.09 CS.seek 7.5ml @14:43:48.70 CSV.dial :waste @14:43:50.35 CS.reconfigure CSconfig @14:43:50.41 CS.seek 0ml @14:44:10.95 Flushing lower manifold's waste1 port @14:44:11.09 CSV.dial :flush @14:44:11.65 CS.reconfigure CSfast @14:44:11.72 CS.seek 5ml @14:44:18.32 delay 3 seconds @14:44:21.34 Eluate primed @14:44:21.35 ATV.dial :waste2 @14:44:21.99 AS.reconfigure ASnorm @14:44:22.05 AS.seek 0ml @14:44:25.70
delay 5 seconds @14:44:30.72 CSV.dial :air @14:44:31.30 CS.seek 7ml @14:44:37.45 CSV.dial CTV @14:44:37.48
CTV.dial CSV @14:44:39.13 CBV.dial :waste1 @14:44:40.03 CS.seek 0ml @14:44:56.84 SPE.coast @14:44:56.93 ASV.dial :SPErecovery @14:44:56.95 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118aug1514h.pcr @14:44:56.97 Building PCR Slug from ASV with MM2 mix, PP4 primer @14:44:57.00 Priming with carrier @14:44:57.02 ATV.dial ARV @14:44:58.01 ARV.dial :carrier @14:44:58.37 AS.seek 0.5ml @14:44:59.29
Flushing lower manifold's waste2b port @14:44:59.93 CSV.dial :flush @14:45:00.80 CS.seek 5ml @14:45:14.67 delay 5 seconds @14:45:19.69 CSV.dial :air @14:45:20.29 CS.seek 7.001ml @14:45:23.94 AFV.select :carrierSolution @14:45:24.06 delay 2 seconds @14:45:26.08 AS.reconfigure ASconfig @14:45:26.15 AS.seek 1ml @14:45:26.42 CSV.dial CTV @14:45:26.45
CTV.dial CSV @14:45:26.96 CBV.dial :waste2b @14:45:27.84 CS.seek 0ml @14:45:46.54 Flushing lower manifold's waste1 port @14:45:46.55 CSV.dial CSR @14:45:47.13 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @14:45:47.28 CS.reconfigure CSconfig @14:45:47.34 CS.seek 4ml @14:45:55.20 delay 1 second @14:45:56.22 AFV.select :holdingCoil @14:45:56.33 delay 1 second @14:45:57.35 ATV.dial :waste @14:45:58.63
delay 5 seconds @14:45:58.82 AS.reconfigure ASnorm @14:45:58.88 AS.seek :empty @14:46:03.65
CSR.select :air @14:46:03.77 CS.reconfigure CSfast @14:46:03.83 CS.seek 10ml @14:46:20.13 CSV.dial CTV @14:46:20.16
CTV.dial CSV @14:46:20.70 CBV.dial :waste1 @14:46:21.60 CS.seek 0ml @14:46:47.95 Flushing lower manifold's waste2b port @14:46:47.96 CSV.dial CSR @14:46:48.54 Pulling 4ml of CSR.mfbkill @14:46:48.55 CSR.select :mfbkill @14:46:48.70 CS.reconfigure CSconfig @14:46:48.76 CS.seek 4ml @14:46:49.61 AFV.select :carrierSolution @14:46:49.74 delay 2 seconds @14:46:51.76 AS.reconfigure ASconfig @14:46:51.83 AS.seek 0.5ml @14:47:00.01
delay 5 seconds @14:47:05.03 CSR.select :air @14:47:05.17 CS.reconfigure CSfast @14:47:05.23 CS.seek 10ml @14:47:20.93 delay 1 second @14:47:21.35 CSV.dial CTV @14:47:21.38
CTV.dial CSV @14:47:21.92 CBV.dial :waste2b @14:47:21.99 AFV.select :holdingCoil @14:47:22.09 Building Slug @14:47:22.10 ATV.dial :ARV @14:47:22.81
CS.seek 0ml @14:47:23.73 delay 2 seconds @14:47:25.75 ARV.dial :air1 @14:47:26.15 delay 2 seconds @14:47:28.22 AS.reconfigure ASslow2 @14:47:28.28 AS.seek 0.506ml @14:47:34.51 delay 2 seconds @14:47:36.53 ARV.dial :MM2 @14:47:38.06 delay 2 seconds @14:47:40.08 Adding MM2 @14:47:40.16 AS.seek 0.524ml @14:47:49.04
Killing upper manifold @14:47:49.05 CSV.dial CSR @14:47:49.64 Pulling 4ml of CSR.mfbkill @14:47:49.65 CSR.select :mfbkill @14:47:49.80 CS.reconfigure CSconfig @14:47:49.86 CS.seek 4ml @14:47:58.60 delay 25 seconds @14:48:01.17
delay 5 seconds @14:48:06.19 CSR.select :air @14:48:06.32 CS.reconfigure CSfast @14:48:06.38 CS.seek 10ml @14:48:22.54 CSV.dial CBV @14:48:22.57
CBV.dial CSV @14:48:23.62 ARV.dial :PP4 @14:48:23.79
CTV.dial :air @14:48:24.03 Adding PP4 @14:48:24.04 delay 2 seconds @14:48:25.32
CS.seek 0ml @14:48:26.61 AS.seek 0.53ml @14:48:33.04 delay 10 seconds @14:48:43.07 ARV.dial :air3 @14:48:43.48 adding ARV air @14:48:43.49 delay 2 seconds @14:48:45.58 AS.seek 0.536ml @14:48:51.61
delay 7 seconds @14:48:51.79 delay 5 seconds @14:48:56.81 ARV.dial :carrier @14:48:58.12 delay 7 seconds @14:48:58.64
CS.seek 3ml @14:49:05.19 AS.seek 0.594ml @14:49:07.29
CSV.dial :waste @14:49:07.79 CS.seek 0ml @14:49:16.39 Flushing upper manifold @14:49:16.53 CSV.dial :flush @14:49:17.07 CS.seek 5ml @14:49:30.79 delay 5 seconds @14:49:35.81 CSV.dial :air @14:49:36.37 CS.seek 7ml @14:49:42.54 CSV.dial CBV @14:49:42.57
CBV.dial CSV @14:49:43.76 CTV.dial :air @14:49:43.78 CS.seek 0ml @14:49:57.36 Session Begun @14:50:02.55
delay 7 seconds @14:50:03.71 delay 5 seconds @14:50:08.73 ATV.dial :ASV @14:50:09.24 delay 2 seconds @14:50:09.58
CS.seek 3ml @14:50:11.31 AS.seek 0.6ml @14:50:12.34 -> showlog 100 @14:50:17.99 delay 4 seconds @14:50:18.23
CSV.dial :waste @14:50:18.76 CS.seek 0ml @14:50:22.01 ATV.dial ARV @14:50:22.65 delay 2 seconds @14:50:24.67 ARV.dial :carrier @14:50:24.68 delay 2 seconds @14:50:26.77 AS.seek 0.65ml @14:50:27.65
CSV.dial :flush @14:50:28.19 CS.seek 3ml @14:50:36.92 delay 5 seconds @14:50:41.95 CSV.dial :air @14:50:42.53 CS.seek 5.001ml @14:50:48.61 CSV.dial :air @14:50:48.63 CS.seek 0ml @14:51:02.29 delay 7 seconds @14:51:09.32 CS.seek 3ml @14:51:17.48 delay 4 seconds @14:51:18.00
CSV.dial :waste @14:51:18.88 CS.seek 0ml @14:51:21.50 ATV.dial PCR @14:51:22.12 ASV.dial :waste1 @14:51:22.94 ARV.dial :air1 @14:51:23.34 Positioning Slug by 0.324ml @14:51:23.44 AS.seek 0.326ml @14:51:27.55
Flushing lower manifold's waste1 port @14:51:27.70 CSV.dial :flush @14:51:28.24 CS.seek 5ml @14:51:41.98 delay 5 seconds @14:51:47.00 CSV.dial :air @14:51:47.59 CS.seek 7ml @14:51:53.61 CSV.dial CTV @14:51:53.64
CTV.dial CSV @14:51:55.28 CBV.dial :waste1 @14:51:55.81 CS.seek 0ml @14:52:14.39 Flushing lower manifold's waste2b port @14:52:14.54 CSV.dial :flush @14:52:15.41 CS.seek 5ml @14:52:29.83 delay 5 seconds @14:52:34.85 CSV.dial :air @14:52:35.43 CS.seek 7ml @14:52:41.45 CSV.dial CTV @14:52:41.48
CTV.dial CSV @14:52:42.01 CBV.dial :waste2b @14:52:42.91 CS.seek 0ml @14:53:01.52 Garaging FlushPuck in Collection Clamp @14:53:01.63 Elbow.reconfigure TightElbow @14:53:01.70 Elbow.seek CC @14:53:01.98 Elbow.reconfigure LooseElbow @14:53:02.10 Forearm.seek CC @14:53:03.06 Hand.close @14:53:03.87 CC.seek :open @14:53:08.28 Forearm.seek :retract @14:53:09.29 Elbow.reconfigure TightElbow @14:53:09.36 Elbow.seek :garage @14:53:12.45 Elbow.reconfigure LooseElbow @14:53:12.58 Forearm.reconfigure TightForearm @14:53:12.65 Forearm.seek :garage @14:53:13.83 Hand.open @14:53:14.68 Forearm.reconfigure LooseForearm @14:53:14.75 Forearm.seek :retract @14:53:15.81 Starting initialPurge @14:53:15.82 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:53:15.93 Hand.open @14:53:16.31 Elbow.reconfigure TightElbow @14:53:16.39 Elbow.seek FlushPuck::Garage @14:53:16.60 Elbow.reconfigure LooseElbow @14:53:16.72 Forearm.seek :garage @14:53:17.76 Hand.close @14:53:18.62 Forearm.seek :retract @14:53:19.68 Elbow.reconfigure TightElbow @14:53:19.75 Elbow.seek CC @14:53:22.69 Elbow.reconfigure LooseElbow @14:53:22.81 Forearm.reconfigure TightForearm @14:53:22.88 Forearm.seek CC @14:53:24.05 CC.seek :closed @14:53:28.32 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.19 @14:53:28.42 Hand.open @14:53:29.18 Forearm.reconfigure LooseForearm @14:53:29.25 Forearm.seek :retract @14:53:30.23 Flushing the Collection Valve::Manifold @14:53:30.38 CSV.dial CSR @14:53:30.97 Pulling 5ml of CSR.flush @14:53:30.98 CSR.select :flush @14:53:31.14 CS.reconfigure CSconfig @14:53:31.20 CS.seek 5ml @14:53:45.05 delay 5 seconds @14:53:50.07 CSR.select :air @14:53:50.26 CS.reconfigure CSfast @14:53:50.33 CS.seek 7.501ml @14:53:57.73 CSV.dial :waste @14:53:59.25 CS.reconfigure CSconfig @14:53:59.33 CS.seek 0ml @14:54:20.00 Purging external Intake @14:54:20.19 CSV.dial :flush @14:54:20.70 CS.reconfigure CSfast @14:54:20.77 CS.seek 10ml @14:54:47.05 delay 5 seconds @14:54:52.07 CSV.dial CBV @14:54:52.11
CBV.dial CSV @14:54:53.03 CTV.dial :intake @14:54:53.91 Intake.select :open @14:54:54.05 CS.seek 0ml @14:55:21.30 Intake.select :close @14:55:21.43 CTV.dial :air @14:55:22.31 Flushing upper manifold @14:55:22.45 CSV.dial :flush @14:55:23.45 CS.seek 5ml @14:55:37.14 delay 5 seconds @14:55:42.16 CSV.dial :air @14:55:42.78 CS.seek 7ml @14:55:48.86 CSV.dial CBV @14:55:48.89
CBV.dial CSV @14:55:50.13 CTV.dial :air @14:55:50.15 CS.seek 0ml @14:56:08.74 delay 7 seconds @14:56:15.77 CS.seek 3ml @14:56:24.42 CSV.dial :waste @14:56:24.97 CS.seek 0ml @14:56:33.63 CSV.dial :flush @14:56:34.15 CS.seek 3ml @14:56:42.74 delay 5 seconds @14:56:47.76 CSV.dial :air @14:56:48.47 CS.seek 5ml @14:56:49.59 delay 5 seconds @14:56:54.46
CSV.dial :air @14:56:54.48 CS.seek 0ml @14:56:54.61 ATV.dial ARV @14:56:55.39 ASV.dial :PCRlock @14:56:55.78 delay 5 seconds @14:57:00.80 Running Tt1 Profile60C300 thermocycling @14:57:00.82 PCR.program Profile60C300 @14:57:00.98 Profile60C300 should complete in < 1:45:21 @14:57:00.99 PCR.run 2:16:41.25 @14:57:08.08
delay 7 seconds @14:57:15.11 CS.seek 3ml @14:57:15.49 Session Ended @14:57:23.76
CSV.dial :waste @14:57:24.61 CS.seek 0ml @14:57:33.18 Garaging FlushPuck in Collection Clamp @14:57:33.27 Elbow.reconfigure TightElbow @14:57:33.33 Elbow.seek CC @14:57:33.60 Elbow.reconfigure LooseElbow @14:57:33.72 Forearm.seek CC @14:57:34.72 Hand.close @14:57:35.50 CC.seek :open @14:57:39.91 Forearm.seek :retract @14:57:40.92 Elbow.reconfigure TightElbow @14:57:40.98 Elbow.seek :garage @14:57:44.21 Elbow.reconfigure LooseElbow @14:57:44.32 Forearm.reconfigure TightForearm @14:57:44.38 Forearm.seek :garage @14:57:45.52 Hand.open @14:57:46.31 Forearm.reconfigure LooseForearm @14:57:46.37 Forearm.seek :retract @14:57:47.36 Completed [coreNegNTCphase] begun 13:00:50 @14:57:47.38 Extending 18tun until completion of MFBPCR @16:17:25.16 PCR.off @16:17:26.67 PCR for Tt1 completed -- closed /var/log/esp/Tt118aug1514h.pcr @16:17:26.75 Cleaning PCR @16:17:26.76 AFV.select :carrierSolution @16:17:26.88 delay 2 seconds @16:17:28.90 AS.reconfigure ASconfig @16:17:28.96 AS.seek 0.5ml @16:17:39.28 delay 1 second @16:17:40.30 AFV.select :holdingCoil @16:17:40.40 ASV.dial :waste1 @16:17:40.79 ATV.dial :air3 @16:17:42.06 AS.reconfigure ASslow1 @16:17:42.12 AS.seek 0.51ml @16:17:44.40 delay 2 seconds @16:17:46.43 ATV.dial :bleach @16:17:47.06 AS.seek 0.81ml @16:18:47.64 delay 3 seconds @16:18:50.66 ATV.dial :air3 @16:18:51.20 delay 3 seconds @16:18:54.27 AS.seek 0.835ml @16:18:59.62 delay 3 seconds @16:19:02.64 ATV.dial :bleach @16:19:03.26 AS.seek 0.82ml @16:19:06.59 delay 3 seconds @16:19:09.61 ARV.dial :waste @16:19:10.73 delay 3 seconds @16:19:13.75 ATV.dial :ARV @16:19:14.62 AS.reconfigure ASconfig @16:19:14.68 AS.seek 0.47ml @16:19:35.08 AFV.select :carrierSolution @16:19:35.20 delay 2 seconds @16:19:37.23 AS.seek 1ml @16:20:08.03 delay 1 second @16:20:09.05 AFV.select :holdingCoil @16:20:09.15 delay 1 second @16:20:10.17 AS.reconfigure ASfast @16:20:10.24 AS.seek 0ml @16:20:37.72 AFV.select :carrierSolution @16:20:37.85 delay 2 seconds @16:20:39.87 AS.reconfigure ASconfig @16:20:39.93 AS.seek 1ml @16:21:37.70 delay 1 second @16:21:38.72 AFV.select :holdingCoil @16:21:38.82 delay 1 second @16:21:39.84 AS.reconfigure ASfast @16:21:39.90 AS.seek 0ml @16:22:07.42 AFV.select :carrierSolution @16:22:07.55 delay 2 seconds @16:22:09.57 AS.reconfigure ASconfig @16:22:09.63 AS.seek 1ml @16:23:07.39 delay 1 second @16:23:08.41 AFV.select :holdingCoil @16:23:08.51 delay 1 second @16:23:09.53 AS.reconfigure ASfast @16:23:09.60 AS.seek 0ml @16:23:36.97 ARV.dial between carrier and air1 @16:23:37.50 ATV.dial :air3 @16:23:38.78 AS.reconfigure ASslow1 @16:23:38.84 AS.seek 0.01ml @16:23:41.15 delay 2 seconds @16:23:43.17 ATV.dial :bleach @16:23:43.79 AS.seek 0.81ml @16:26:24.94 delay 3 seconds @16:26:27.96 ATV.dial :air3 @16:26:28.51 delay 3 seconds @16:26:31.58 AS.seek 0.835ml @16:26:36.90 delay 3 seconds @16:26:39.92 ATV.dial :bleach @16:26:40.54 AS.seek 0.82ml @16:26:43.87 delay 3 seconds @16:26:46.89 ATV.dial PCR @16:26:47.30 AS.reconfigure ASconfig @16:26:47.36 AS.seek :empty @16:27:34.83 PCR.program :bleachProfile @16:27:34.88 PCR program should complete in < 2:45 @16:27:34.89 PCR.run 10:00 @16:30:36.73 PCR.off @16:30:36.76 AFV.select :carrierSolution @16:30:36.88 delay 2 seconds @16:30:38.91 AS.seek 1ml @16:31:36.75 delay 1 second @16:31:37.77 AFV.select :holdingCoil @16:31:37.87 delay 1 second @16:31:38.89 AS.reconfigure ASfast @16:31:38.95 AS.seek 0ml @16:32:06.53 delay 3 seconds @16:32:09.55 AFV.select :carrierSolution @16:32:09.65 delay 2 seconds @16:32:11.67 AS.reconfigure ASconfig @16:32:11.74 AS.seek 1ml @16:33:09.50 delay 1 second @16:33:10.52 AFV.select :holdingCoil @16:33:10.62 delay 1 second @16:33:11.64 AS.reconfigure ASfast @16:33:11.70 AS.seek 0ml @16:33:39.16 delay 3 seconds @16:33:42.18 AFV.select :carrierSolution @16:33:42.28 delay 2 seconds @16:33:44.30 AS.reconfigure ASconfig @16:33:44.38 AS.seek 1ml @16:34:42.19 delay 1 second @16:34:43.21 AFV.select :holdingCoil @16:34:43.31 delay 1 second @16:34:44.33 AS.reconfigure ASfast @16:34:44.39 AS.seek 0ml @16:35:11.84 delay 3 seconds @16:35:14.86 AFV.select :carrierSolution @16:35:14.96 delay 2 seconds @16:35:16.98 AS.reconfigure ASconfig @16:35:17.04 AS.seek 1ml @16:36:14.83 delay 1 second @16:36:15.85 AFV.select :holdingCoil @16:36:15.95 delay 1 second @16:36:16.97 AS.reconfigure ASfast @16:36:17.03 AS.seek 0ml @16:36:44.41 delay 3 seconds @16:36:47.43 AFV.select :carrierSolution @16:36:47.53 delay 2 seconds @16:36:49.55 AS.reconfigure ASconfig @16:36:49.62 AS.seek 1ml @16:37:47.39 delay 1 second @16:37:48.41 AFV.select :holdingCoil @16:37:48.51 delay 1 second @16:37:49.53 AS.reconfigure ASnorm @16:37:49.59 AS.seek 0ml @16:38:40.80 Setting up PCR for IPc -- writing /var/log/esp/IPc18aug1516h.pcr @16:38:40.81 Building PCR Slug from ASV with MM1 mix, PP1 primer @16:38:40.84 Priming with carrier @16:38:40.86 ATV.dial ARV @16:38:41.58 ARV.dial :carrier @16:38:41.94 AS.seek 0.5ml @16:39:07.49 AFV.select :carrierSolution @16:39:07.62 delay 2 seconds @16:39:09.64 AS.reconfigure ASconfig @16:39:09.71 AS.seek 1ml @16:39:38.74 delay 1 second @16:39:39.76 AFV.select :holdingCoil @16:39:39.86 delay 1 second @16:39:40.88 ATV.dial :waste @16:39:42.61 AS.reconfigure ASnorm @16:39:42.67 AS.seek :empty @16:40:33.44 AFV.select :carrierSolution @16:40:33.57 delay 2 seconds @16:40:35.59 AS.reconfigure ASconfig @16:40:35.66 AS.seek 0.5ml @16:41:04.80 delay 1 second @16:41:05.82 AFV.select :holdingCoil @16:41:05.92 Building Slug @16:41:05.94 ATV.dial :ARV @16:41:07.66 delay 2 seconds @16:41:09.68 ARV.dial :air1 @16:41:10.08 delay 2 seconds @16:41:12.15 AS.reconfigure ASslow2 @16:41:12.21 AS.seek 0.506ml @16:41:18.63 delay 2 seconds @16:41:20.65 ARV.dial :MM1 @16:41:21.25 delay 2 seconds @16:41:23.27 Adding MM1 @16:41:23.35 AS.seek 0.524ml @16:41:41.72 delay 25 seconds @16:42:06.74 ARV.dial :PP1 @16:42:07.14 Adding PP1 @16:42:07.15 delay 2 seconds @16:42:09.24 AS.seek 0.53ml @16:42:15.58 delay 10 seconds @16:42:25.61 ARV.dial :air1 @16:42:26.00 adding ARV air @16:42:26.01 delay 2 seconds @16:42:28.09 AS.seek 0.536ml @16:42:34.63 delay 5 seconds @16:42:39.65 ARV.dial :carrier @16:42:40.06 delay 7 seconds @16:42:47.14 AS.seek 0.594ml @16:43:45.77 delay 5 seconds @16:43:50.79 ATV.dial :ASV @16:43:51.21 delay 2 seconds @16:43:53.28 AS.seek 0.6ml @16:43:59.77 delay 4 seconds @16:44:03.79 ATV.dial ARV @16:44:04.33 delay 2 seconds @16:44:06.35 ARV.dial :carrier @16:44:06.36 delay 2 seconds @16:44:08.44 AS.seek 0.65ml @16:44:59.00 delay 4 seconds @16:45:03.02 ATV.dial PCR @16:45:03.63 ASV.dial :waste1 @16:45:03.64 ARV.dial :air1 @16:45:04.07 Positioning Slug by 0.324ml @16:45:04.17 AS.seek 0.326ml @16:50:30.47 delay 5 seconds @16:50:35.49 ATV.dial ARV @16:50:36.25 ASV.dial :PCRlock @16:50:36.64 delay 5 seconds @16:50:41.66 Running IPc ProfileIPc thermocycling @16:50:41.68 PCR.program ProfileIPc @16:50:41.83 ProfileIPc should complete in < 1:50:49 @16:50:41.84 PCR.run 2:23:31.25 @18:13:34.94 PCR.off @18:13:37.94 PCR for IPc completed -- closed /var/log/esp/IPc18aug1516h.pcr @18:13:38.01 Cleaning PCR @18:13:38.02 AFV.select :carrierSolution @18:13:38.15 delay 2 seconds @18:13:40.17 AS.reconfigure ASconfig @18:13:40.24 AS.seek 0.5ml @18:13:50.57 delay 1 second @18:13:51.59 AFV.select :holdingCoil @18:13:51.69 ASV.dial :waste1 @18:13:52.08 ATV.dial :air3 @18:13:53.36 AS.reconfigure ASslow1 @18:13:53.42 AS.seek 0.51ml @18:13:55.71 delay 2 seconds @18:13:57.74 ATV.dial :bleach @18:13:58.36 AS.seek 0.81ml @18:14:58.96 delay 3 seconds @18:15:01.98 ATV.dial :air3 @18:15:02.52 delay 3 seconds @18:15:05.59 AS.seek 0.835ml @18:15:10.89 delay 3 seconds @18:15:13.91 ATV.dial :bleach @18:15:14.53 AS.seek 0.82ml @18:15:17.88 delay 3 seconds @18:15:20.90 ARV.dial :waste @18:15:21.54 delay 3 seconds @18:15:24.56 ATV.dial :ARV @18:15:25.55 AS.reconfigure ASconfig @18:15:25.61 AS.seek 0.47ml @18:15:46.00 AFV.select :carrierSolution @18:15:46.12 delay 2 seconds @18:15:48.15 AS.seek 1ml @18:16:18.90 delay 1 second @18:16:19.92 AFV.select :holdingCoil @18:16:20.02 delay 1 second @18:16:21.04 AS.reconfigure ASfast @18:16:21.10 AS.seek 0ml @18:16:48.73 AFV.select :carrierSolution @18:16:48.85 delay 2 seconds @18:16:50.87 AS.reconfigure ASconfig @18:16:50.93 AS.seek 1ml @18:17:48.71 delay 1 second @18:17:49.73 AFV.select :holdingCoil @18:17:49.83 delay 1 second @18:17:50.85 AS.reconfigure ASfast @18:17:50.92 AS.seek 0ml @18:18:18.37 AFV.select :carrierSolution @18:18:18.49 delay 2 seconds @18:18:20.51 AS.reconfigure ASconfig @18:18:20.57 AS.seek 1ml @18:19:18.33 delay 1 second @18:19:19.35 AFV.select :holdingCoil @18:19:19.45 delay 1 second @18:19:20.47 AS.reconfigure ASfast @18:19:20.54 AS.seek 0ml @18:19:47.95 ARV.dial between carrier and air1 @18:19:48.48 ATV.dial :air3 @18:19:49.74 AS.reconfigure ASslow1 @18:19:49.81 AS.seek 0.01ml @18:19:52.15 delay 2 seconds @18:19:54.17 ATV.dial :bleach @18:19:54.80 AS.seek 0.81ml @18:22:35.93 delay 3 seconds @18:22:38.95 ATV.dial :air3 @18:22:39.60 delay 3 seconds @18:22:42.67 AS.seek 0.835ml @18:22:47.98 delay 3 seconds @18:22:51.00 ATV.dial :bleach @18:22:51.61 AS.seek 0.82ml @18:22:54.88 delay 3 seconds @18:22:57.90 ATV.dial PCR @18:22:58.30 AS.reconfigure ASconfig @18:22:58.36 AS.seek :empty @18:23:45.83 PCR.program :bleachProfile @18:23:45.89 PCR program should complete in < 2:45 @18:23:45.91 PCR.run 10:00 @18:26:47.75 PCR.off @18:26:47.78 AFV.select :carrierSolution @18:26:47.90 delay 2 seconds @18:26:49.93 AS.seek 1ml @18:27:47.73 delay 1 second @18:27:48.75 AFV.select :holdingCoil @18:27:48.85 delay 1 second @18:27:49.87 AS.reconfigure ASfast @18:27:49.93 AS.seek 0ml @18:28:17.71 delay 3 seconds @18:28:20.73 AFV.select :carrierSolution @18:28:20.83 delay 2 seconds @18:28:22.86 AS.reconfigure ASconfig @18:28:23.44 AS.seek 1ml @18:29:21.24 delay 1 second @18:29:22.26 AFV.select :holdingCoil @18:29:22.36 delay 1 second @18:29:23.38 AS.reconfigure ASfast @18:29:23.44 AS.seek 0ml @18:29:50.99 delay 3 seconds @18:29:54.01 AFV.select :carrierSolution @18:29:54.11 delay 2 seconds @18:29:56.13 AS.reconfigure ASconfig @18:29:56.20 AS.seek 1ml @18:30:53.98 delay 1 second @18:30:55.00 AFV.select :holdingCoil @18:30:55.10 delay 1 second @18:30:56.12 AS.reconfigure ASfast @18:30:56.19 AS.seek 0ml @18:31:23.70 delay 3 seconds @18:31:26.72 AFV.select :carrierSolution @18:31:26.82 delay 2 seconds @18:31:28.84 AS.reconfigure ASconfig @18:31:28.91 AS.seek 1ml @18:32:26.70 delay 1 second @18:32:27.72 AFV.select :holdingCoil @18:32:27.82 delay 1 second @18:32:28.84 AS.reconfigure ASfast @18:32:28.91 AS.seek 0ml @18:32:56.29 delay 3 seconds @18:32:59.31 AFV.select :carrierSolution @18:32:59.41 delay 2 seconds @18:33:01.43 AS.reconfigure ASconfig @18:33:01.50 AS.seek 1ml @18:33:59.29 delay 1 second @18:34:00.31 AFV.select :holdingCoil @18:34:00.41 delay 1 second @18:34:01.43 AS.reconfigure ASnorm @18:34:01.49 AS.seek 0ml @18:34:52.23 Setting up PCR for Rp -- writing /var/log/esp/Rp18aug1518h.pcr @18:34:52.24 Building PCR Slug from ASV with MM2 mix, PP3 primer @18:34:52.27 Priming with carrier @18:34:52.29 ATV.dial ARV @18:34:52.92 ARV.dial :carrier @18:34:53.27 AS.seek 0.5ml @18:35:18.84 AFV.select :carrierSolution @18:35:18.97 delay 2 seconds @18:35:20.99 AS.reconfigure ASconfig @18:35:21.06 AS.seek 1ml @18:35:50.10 delay 1 second @18:35:51.12 AFV.select :holdingCoil @18:35:51.22 delay 1 second @18:35:52.24 ATV.dial :waste @18:35:53.87 AS.reconfigure ASnorm @18:35:53.93 AS.seek :empty @18:36:44.70 AFV.select :carrierSolution @18:36:44.83 delay 2 seconds @18:36:46.85 AS.reconfigure ASconfig @18:36:46.92 AS.seek 0.5ml @18:37:16.03 delay 1 second @18:37:17.05 AFV.select :holdingCoil @18:37:17.15 Building Slug @18:37:17.16 ATV.dial :ARV @18:37:18.79 delay 2 seconds @18:37:20.81 ARV.dial :air1 @18:37:21.22 delay 2 seconds @18:37:23.29 AS.reconfigure ASslow2 @18:37:23.36 AS.seek 0.506ml @18:37:29.57 delay 2 seconds @18:37:31.59 ARV.dial :MM2 @18:37:33.10 delay 2 seconds @18:37:35.12 Adding MM2 @18:37:35.20 AS.seek 0.524ml @18:37:53.65 delay 25 seconds @18:38:18.67 ARV.dial :PP3 @18:38:19.07 Adding PP3 @18:38:19.08 delay 2 seconds @18:38:21.16 AS.seek 0.53ml @18:38:27.53 delay 10 seconds @18:38:37.56 ARV.dial :air2 @18:38:37.96 adding ARV air @18:38:37.97 delay 2 seconds @18:38:40.05 AS.seek 0.536ml @18:38:46.46 delay 5 seconds @18:38:51.48 ARV.dial :carrier @18:38:52.78 delay 7 seconds @18:38:59.86 AS.seek 0.594ml @18:39:58.51 delay 5 seconds @18:40:03.53 ATV.dial :ASV @18:40:03.93 delay 2 seconds @18:40:06.00 AS.seek 0.6ml @18:40:12.26 delay 4 seconds @18:40:16.28 ATV.dial ARV @18:40:16.82 delay 2 seconds @18:40:18.84 ARV.dial :carrier @18:40:18.85 delay 2 seconds @18:40:20.93 AS.seek 0.65ml @18:41:11.56 delay 4 seconds @18:41:15.58 ATV.dial PCR @18:41:16.18 ASV.dial :waste1 @18:41:16.19 ARV.dial :air1 @18:41:16.63 Positioning Slug by 0.324ml @18:41:16.72 AS.seek 0.326ml @18:46:43.04 delay 5 seconds @18:46:48.06 ATV.dial ARV @18:46:49.34 ASV.dial :PCRlock @18:46:49.73 delay 5 seconds @18:46:54.75 Running Rp Profile60C300 thermocycling @18:46:54.76 PCR.program Profile60C300 @18:46:54.91 Profile60C300 should complete in < 1:45:21 @18:46:54.92 PCR.run 2:16:41.25 @20:07:45.12 PCR.off @20:07:46.63 PCR for Rp completed -- closed /var/log/esp/Rp18aug1518h.pcr @20:07:46.72 Cleaning PCR @20:07:46.73 AFV.select :carrierSolution @20:07:46.85 delay 2 seconds @20:07:48.87 AS.reconfigure ASconfig @20:07:48.93 AS.seek 0.5ml @20:07:59.25 delay 1 second @20:08:00.27 AFV.select :holdingCoil @20:08:00.38 ASV.dial :waste1 @20:08:00.76 ATV.dial :air3 @20:08:02.03 AS.reconfigure ASslow1 @20:08:02.10 AS.seek 0.51ml @20:08:04.37 delay 2 seconds @20:08:06.40 ATV.dial :bleach @20:08:07.52 AS.seek 0.81ml @20:09:08.14 delay 3 seconds @20:09:11.16 ATV.dial :air3 @20:09:11.70 delay 3 seconds @20:09:14.77 AS.seek 0.835ml @20:09:20.00 delay 3 seconds @20:09:23.02 ATV.dial :bleach @20:09:23.65 AS.seek 0.82ml @20:09:26.97 delay 3 seconds @20:09:29.99 ARV.dial :waste @20:09:30.62 delay 3 seconds @20:09:33.64 ATV.dial :ARV @20:09:34.53 AS.reconfigure ASconfig @20:09:34.59 AS.seek 0.47ml @20:09:54.99 AFV.select :carrierSolution @20:09:55.11 delay 2 seconds @20:09:57.14 AS.seek 1ml @20:10:27.95 delay 1 second @20:10:28.97 AFV.select :holdingCoil @20:10:29.07 delay 1 second @20:10:30.09 AS.reconfigure ASfast @20:10:30.16 AS.seek 0ml @20:10:57.63 AFV.select :carrierSolution @20:10:57.75 delay 2 seconds @20:10:59.77 AS.reconfigure ASconfig @20:10:59.83 AS.seek 1ml @20:11:57.59 delay 1 second @20:11:58.61 AFV.select :holdingCoil @20:11:58.71 delay 1 second @20:11:59.73 AS.reconfigure ASfast @20:11:59.79 AS.seek 0ml @20:12:27.32 AFV.select :carrierSolution @20:12:27.44 delay 2 seconds @20:12:29.46 AS.reconfigure ASconfig @20:12:29.52 AS.seek 1ml @20:13:27.30 delay 1 second @20:13:28.32 AFV.select :holdingCoil @20:13:28.42 delay 1 second @20:13:29.44 AS.reconfigure ASfast @20:13:29.50 AS.seek 0ml @20:13:56.88 ARV.dial between carrier and air1 @20:13:57.39 ATV.dial :air3 @20:13:58.67 AS.reconfigure ASslow1 @20:13:58.73 AS.seek 0.01ml @20:14:01.11 delay 2 seconds @20:14:03.13 ATV.dial :bleach @20:14:03.75 AS.seek 0.81ml @20:16:44.91 delay 3 seconds @20:16:47.93 ATV.dial :air3 @20:16:48.47 delay 3 seconds @20:16:51.54 AS.seek 0.835ml @20:16:56.77 delay 3 seconds @20:16:59.79 ATV.dial :bleach @20:17:00.40 AS.seek 0.82ml @20:17:03.69 delay 3 seconds @20:17:06.71 ATV.dial PCR @20:17:07.12 AS.reconfigure ASconfig @20:17:07.18 AS.seek :empty @20:17:54.66 PCR.program :bleachProfile @20:17:54.72 PCR program should complete in < 2:45 @20:17:54.73 PCR.run 10:00 @20:20:56.57 PCR.off @20:20:56.60 AFV.select :carrierSolution @20:20:56.72 delay 2 seconds @20:20:58.75 AS.seek 1ml @20:21:56.56 delay 1 second @20:21:57.58 AFV.select :holdingCoil @20:21:57.68 delay 1 second @20:21:58.70 AS.reconfigure ASfast @20:21:58.76 AS.seek 0ml @20:22:26.25 delay 3 seconds @20:22:29.27 AFV.select :carrierSolution @20:22:29.37 delay 2 seconds @20:22:31.39 AS.reconfigure ASconfig @20:22:31.46 AS.seek 1ml @20:23:29.22 delay 1 second @20:23:30.24 AFV.select :holdingCoil @20:23:30.34 delay 1 second @20:23:31.36 AS.reconfigure ASfast @20:23:31.42 AS.seek 0ml @20:23:58.77 delay 3 seconds @20:24:01.79 AFV.select :carrierSolution @20:24:01.89 delay 2 seconds @20:24:03.91 AS.reconfigure ASconfig @20:24:03.98 AS.seek 1ml @20:25:01.73 delay 1 second @20:25:02.75 AFV.select :holdingCoil @20:25:02.85 delay 1 second @20:25:03.87 AS.reconfigure ASfast @20:25:03.93 AS.seek 0ml @20:25:31.50 delay 3 seconds @20:25:34.52 AFV.select :carrierSolution @20:25:34.62 delay 2 seconds @20:25:36.64 AS.reconfigure ASconfig @20:25:36.72 AS.seek 1ml @20:26:34.47 delay 1 second @20:26:35.49 AFV.select :holdingCoil @20:26:36.12 delay 1 second @20:26:37.14 AS.reconfigure ASfast @20:26:37.20 AS.seek 0ml @20:27:04.97 delay 3 seconds @20:27:07.99 AFV.select :carrierSolution @20:27:08.09 delay 2 seconds @20:27:10.11 AS.reconfigure ASconfig @20:27:10.18 AS.seek 1ml @20:28:07.94 delay 1 second @20:28:08.96 AFV.select :holdingCoil @20:28:09.06 delay 1 second @20:28:10.08 AS.reconfigure ASnorm @20:28:10.14 AS.seek 0ml @20:29:00.87 Setting up PCR for Ss -- writing /var/log/esp/Ss18aug1520h.pcr @20:29:00.89 Building PCR Slug from ASV with MM1 mix, PP2 primer @20:29:00.91 Priming with carrier @20:29:00.93 ATV.dial ARV @20:29:01.55 ARV.dial :carrier @20:29:01.91 AS.seek 0.5ml @20:29:27.48 AFV.select :carrierSolution @20:29:27.61 delay 2 seconds @20:29:29.63 AS.reconfigure ASconfig @20:29:29.70 AS.seek 1ml @20:29:58.74 delay 1 second @20:29:59.76 AFV.select :holdingCoil @20:29:59.86 delay 1 second @20:30:00.88 ATV.dial :waste @20:30:02.63 AS.reconfigure ASnorm @20:30:02.70 AS.seek :empty @20:30:53.43 AFV.select :carrierSolution @20:30:53.56 delay 2 seconds @20:30:55.58 AS.reconfigure ASconfig @20:30:55.65 AS.seek 0.5ml @20:31:24.74 delay 1 second @20:31:25.76 AFV.select :holdingCoil @20:31:25.86 Building Slug @20:31:25.87 ATV.dial :ARV @20:31:27.60 delay 2 seconds @20:31:29.62 ARV.dial :air1 @20:31:30.03 delay 2 seconds @20:31:32.10 AS.reconfigure ASslow2 @20:31:32.16 AS.seek 0.506ml @20:31:38.55 delay 2 seconds @20:31:40.57 ARV.dial :MM1 @20:31:41.17 delay 2 seconds @20:31:43.19 Adding MM1 @20:31:43.27 AS.seek 0.524ml @20:32:01.81 delay 25 seconds @20:32:26.83 ARV.dial :PP2 @20:32:27.24 Adding PP2 @20:32:27.25 delay 2 seconds @20:32:29.33 AS.seek 0.53ml @20:32:36.17 delay 10 seconds @20:32:46.20 ARV.dial :air2 @20:32:46.58 adding ARV air @20:32:46.59 delay 2 seconds @20:32:48.67 AS.seek 0.536ml @20:32:54.94 delay 5 seconds @20:32:59.96 ARV.dial :carrier @20:33:01.24 delay 7 seconds @20:33:08.32 AS.seek 0.594ml @20:34:06.85 delay 5 seconds @20:34:11.87 ATV.dial :ASV @20:34:12.28 delay 2 seconds @20:34:14.35 AS.seek 0.6ml @20:34:20.77 delay 4 seconds @20:34:24.79 ATV.dial ARV @20:34:25.35 delay 2 seconds @20:34:27.37 ARV.dial :carrier @20:34:27.38 delay 2 seconds @20:34:29.46 AS.seek 0.65ml @20:35:19.92 delay 4 seconds @20:35:23.94 ATV.dial PCR @20:35:24.55 ASV.dial :waste1 @20:35:24.56 ARV.dial :air1 @20:35:24.99 Positioning Slug by 0.324ml @20:35:25.08 AS.seek 0.326ml @20:40:51.10 delay 5 seconds @20:40:56.12 ATV.dial ARV @20:40:56.89 ASV.dial :PCRlock @20:40:57.27 delay 5 seconds @20:41:02.29 Running Ss Profile60C350 thermocycling @20:41:02.31 PCR.program Profile60C350 @20:41:02.46 Profile60C350 should complete in < 1:48:05 @20:41:02.48 PCR.run 2:20:06.25 @22:02:26.37 PCR.off @22:02:28.35 PCR for Ss completed -- closed /var/log/esp/Ss18aug1520h.pcr @22:02:28.43 Cleaning PCR @22:02:28.44 AFV.select :carrierSolution @22:02:28.56 delay 2 seconds @22:02:30.58 AS.reconfigure ASconfig @22:02:30.64 AS.seek 0.5ml @22:02:40.96 delay 1 second @22:02:41.98 AFV.select :holdingCoil @22:02:42.08 ASV.dial :waste1 @22:02:42.47 ATV.dial :air3 @22:02:43.72 AS.reconfigure ASslow1 @22:02:43.79 AS.seek 0.51ml @22:02:46.10 delay 2 seconds @22:02:48.13 ATV.dial :bleach @22:02:48.73 AS.seek 0.81ml @22:03:49.38 delay 3 seconds @22:03:52.40 ATV.dial :air3 @22:03:53.44 delay 3 seconds @22:03:56.51 AS.seek 0.835ml @22:04:01.83 delay 3 seconds @22:04:04.85 ATV.dial :bleach @22:04:05.47 AS.seek 0.82ml @22:04:08.77 delay 3 seconds @22:04:11.79 ARV.dial :waste @22:04:12.41 delay 3 seconds @22:04:15.43 ATV.dial :ARV @22:04:16.29 AS.reconfigure ASconfig @22:04:16.36 AS.seek 0.47ml @22:04:36.77 AFV.select :carrierSolution @22:04:36.89 delay 2 seconds @22:04:38.92 AS.seek 1ml @22:05:09.71 delay 1 second @22:05:10.73 AFV.select :holdingCoil @22:05:10.84 delay 1 second @22:05:11.86 AS.reconfigure ASfast @22:05:11.93 AS.seek 0ml @22:05:39.28 AFV.select :carrierSolution @22:05:39.40 delay 2 seconds @22:05:41.42 AS.reconfigure ASconfig @22:05:41.48 AS.seek 1ml @22:06:39.28 delay 1 second @22:06:40.30 AFV.select :holdingCoil @22:06:40.40 delay 1 second @22:06:41.42 AS.reconfigure ASfast @22:06:41.48 AS.seek 0ml @22:07:08.90 AFV.select :carrierSolution @22:07:09.03 delay 2 seconds @22:07:11.05 AS.reconfigure ASconfig @22:07:11.11 AS.seek 1ml @22:08:08.88 delay 1 second @22:08:09.90 AFV.select :holdingCoil @22:08:10.00 delay 1 second @22:08:11.02 AS.reconfigure ASfast @22:08:11.08 AS.seek 0ml @22:08:38.50 ARV.dial between carrier and air1 @22:08:39.00 ATV.dial :air3 @22:08:40.27 AS.reconfigure ASslow1 @22:08:40.33 AS.seek 0.01ml @22:08:42.61 delay 2 seconds @22:08:44.63 ATV.dial :bleach @22:08:45.25 AS.seek 0.81ml @22:11:26.39 delay 3 seconds @22:11:29.41 ATV.dial :air3 @22:11:29.94 delay 3 seconds @22:11:33.01 AS.seek 0.835ml @22:11:38.33 delay 3 seconds @22:11:41.35 ATV.dial :bleach @22:11:41.95 AS.seek 0.82ml @22:11:45.21 delay 3 seconds @22:11:48.23 ATV.dial PCR @22:11:48.63 AS.reconfigure ASconfig @22:11:48.69 AS.seek :empty @22:12:36.17 PCR.program :bleachProfile @22:12:36.24 PCR program should complete in < 2:45 @22:12:36.25 PCR.run 10:00 @22:15:35.05 PCR.off @22:15:35.08 AFV.select :carrierSolution @22:15:35.20 delay 2 seconds @22:15:37.23 AS.seek 1ml @22:16:35.05 delay 1 second @22:16:36.07 AFV.select :holdingCoil @22:16:36.17 delay 1 second @22:16:37.19 AS.reconfigure ASfast @22:16:37.25 AS.seek 0ml @22:17:04.61 delay 3 seconds @22:17:07.63 AFV.select :carrierSolution @22:17:07.73 delay 2 seconds @22:17:09.75 AS.reconfigure ASconfig @22:17:09.86 AS.seek 1ml @22:18:07.63 delay 1 second @22:18:08.65 AFV.select :holdingCoil @22:18:08.75 delay 1 second @22:18:09.77 AS.reconfigure ASfast @22:18:09.83 AS.seek 0ml @22:18:37.24 delay 3 seconds @22:18:40.26 AFV.select :carrierSolution @22:18:40.36 delay 2 seconds @22:18:42.38 AS.reconfigure ASconfig @22:18:42.45 AS.seek 1ml @22:19:40.25 delay 1 second @22:19:41.27 AFV.select :holdingCoil @22:19:41.37 delay 1 second @22:19:42.39 AS.reconfigure ASfast @22:19:42.45 AS.seek 0ml @22:20:09.80 delay 3 seconds @22:20:12.82 AFV.select :carrierSolution @22:20:12.92 delay 2 seconds @22:20:14.94 AS.reconfigure ASconfig @22:20:15.00 AS.seek 1ml @22:21:12.75 delay 1 second @22:21:13.77 AFV.select :holdingCoil @22:21:13.87 delay 1 second @22:21:14.89 AS.reconfigure ASfast @22:21:14.95 AS.seek 0ml @22:21:42.38 delay 3 seconds @22:21:45.41 AFV.select :carrierSolution @22:21:45.51 delay 2 seconds @22:21:47.53 AS.reconfigure ASconfig @22:21:47.60 AS.seek 1ml @22:22:45.38 delay 1 second @22:22:46.40 AFV.select :holdingCoil @22:22:46.50 delay 1 second @22:22:47.52 AS.reconfigure ASnorm @22:22:47.58 AS.seek 0ml @22:23:38.30 Setting up PCR for Bb -- writing /var/log/esp/Bb18aug1522h.pcr @22:23:38.32 Building PCR Slug from ASV with MM3 mix, PP5 primer @22:23:38.35 Priming with carrier @22:23:38.37 ATV.dial ARV @22:23:38.99 ARV.dial :carrier @22:23:39.35 AS.seek 0.5ml @22:24:04.93 AFV.select :carrierSolution @22:24:05.06 delay 2 seconds @22:24:07.08 AS.reconfigure ASconfig @22:24:07.15 AS.seek 1ml @22:24:36.19 delay 1 second @22:24:37.21 AFV.select :holdingCoil @22:24:37.31 delay 1 second @22:24:38.33 ATV.dial :waste @22:24:40.05 AS.reconfigure ASnorm @22:24:40.11 AS.seek :empty @22:25:30.85 AFV.select :carrierSolution @22:25:30.98 delay 2 seconds @22:25:33.00 AS.reconfigure ASconfig @22:25:33.07 AS.seek 0.5ml @22:26:02.18 delay 1 second @22:26:03.20 AFV.select :holdingCoil @22:26:03.30 Building Slug @22:26:03.31 ATV.dial :ARV @22:26:05.02 delay 2 seconds @22:26:07.04 ARV.dial :air1 @22:26:07.45 delay 2 seconds @22:26:09.52 AS.reconfigure ASslow2 @22:26:09.58 AS.seek 0.506ml @22:26:15.90 delay 2 seconds @22:26:17.92 ARV.dial :MM3 @22:26:18.97 delay 2 seconds @22:26:20.99 Adding MM3 @22:26:21.07 AS.seek 0.524ml @22:26:39.30 delay 25 seconds @22:27:04.32 ARV.dial :PP5 @22:27:04.72 Adding PP5 @22:27:04.73 delay 2 seconds @22:27:06.82 AS.seek 0.53ml @22:27:13.00 delay 10 seconds @22:27:23.03 ARV.dial :air3 @22:27:23.41 adding ARV air @22:27:23.42 delay 2 seconds @22:27:25.50 AS.seek 0.536ml @22:27:31.77 delay 5 seconds @22:27:36.79 ARV.dial :carrier @22:27:38.08 delay 7 seconds @22:27:45.16 AS.seek 0.594ml @22:28:43.83 delay 5 seconds @22:28:48.85 ATV.dial :ASV @22:28:49.25 delay 2 seconds @22:28:51.32 AS.seek 0.6ml @22:28:57.57 delay 4 seconds @22:29:01.59 ATV.dial ARV @22:29:02.13 delay 2 seconds @22:29:04.15 ARV.dial :carrier @22:29:04.16 delay 2 seconds @22:29:06.24 AS.seek 0.65ml @22:29:56.69 delay 4 seconds @22:30:00.71 ATV.dial PCR @22:30:01.32 ASV.dial :waste1 @22:30:01.33 ARV.dial :air1 @22:30:01.86 Positioning Slug by 0.324ml @22:30:01.95 AS.seek 0.326ml @22:35:28.32 delay 5 seconds @22:35:33.34 ATV.dial ARV @22:35:34.11 ASV.dial :PCRlock @22:35:34.49 delay 5 seconds @22:35:39.51 Running Bb Profile60C300 thermocycling @22:35:39.52 PCR.program Profile60C300 @22:35:39.67 Profile60C300 should complete in < 1:45:21 @22:35:39.68 PCR.run 2:16:41.25 @23:56:20.84 PCR.off @23:56:22.36 PCR for Bb completed -- closed /var/log/esp/Bb18aug1522h.pcr @23:56:22.44 Cleaning PCR @23:56:22.45 AFV.select :carrierSolution @23:56:22.57 delay 2 seconds @23:56:24.59 AS.reconfigure ASconfig @23:56:24.65 AS.seek 0.5ml @23:56:34.96 delay 1 second @23:56:35.98 AFV.select :holdingCoil @23:56:36.08 ASV.dial :waste1 @23:56:36.46 ATV.dial :air3 @23:56:37.73 AS.reconfigure ASslow1 @23:56:37.80 AS.seek 0.51ml @23:56:40.10 delay 2 seconds @23:56:42.13 ATV.dial :bleach @23:56:42.75 AS.seek 0.81ml @23:57:43.35 delay 3 seconds @23:57:46.37 ATV.dial :air3 @23:57:46.93 delay 3 seconds @23:57:50.00 AS.seek 0.835ml @23:57:55.27 delay 3 seconds @23:57:58.29 ATV.dial :bleach @23:57:58.91 AS.seek 0.82ml @23:58:02.22 delay 3 seconds @23:58:05.24 ARV.dial :waste @23:58:05.85 delay 3 seconds @23:58:08.87 ATV.dial :ARV @23:58:09.74 AS.reconfigure ASconfig @23:58:09.80 AS.seek 0.47ml @23:58:30.21 AFV.select :carrierSolution @23:58:30.33 delay 2 seconds @23:58:32.36 AS.seek 1ml @23:59:03.11 delay 1 second @23:59:04.13 AFV.select :holdingCoil @23:59:04.75 delay 1 second @23:59:05.77 AS.reconfigure ASfast @23:59:05.83 AS.seek 0ml @23:59:33.39 AFV.select :carrierSolution @23:59:33.51 delay 2 seconds @23:59:35.53 AS.reconfigure ASconfig @23:59:35.59 AS.seek 1ml @00:00:33.37CEST16-Aug-18 delay 1 second @00:00:34.39 AFV.select :holdingCoil @00:00:34.49 delay 1 second @00:00:35.51 AS.reconfigure ASfast @00:00:35.58 AS.seek 0ml @00:01:02.92 AFV.select :carrierSolution @00:01:03.05 delay 2 seconds @00:01:05.07 AS.reconfigure ASconfig @00:01:05.13 AS.seek 1ml @00:02:02.93 delay 1 second @00:02:03.95 AFV.select :holdingCoil @00:02:04.05 delay 1 second @00:02:05.07 AS.reconfigure ASfast @00:02:05.13 AS.seek 0ml @00:02:32.63 ARV.dial between carrier and air1 @00:02:33.13 ATV.dial :air3 @00:02:34.41 AS.reconfigure ASslow1 @00:02:34.47 AS.seek 0.01ml @00:02:36.79 delay 2 seconds @00:02:38.81 ATV.dial :bleach @00:02:39.44 AS.seek 0.81ml @00:05:20.57 delay 3 seconds @00:05:23.59 ATV.dial :air3 @00:05:24.13 delay 3 seconds @00:05:27.20 AS.seek 0.835ml @00:05:32.53 delay 3 seconds @00:05:35.55 ATV.dial :bleach @00:05:36.16 AS.seek 0.82ml @00:05:39.49 delay 3 seconds @00:05:42.51 ATV.dial PCR @00:05:42.91 AS.reconfigure ASconfig @00:05:42.97 AS.seek :empty @00:06:30.43 PCR.program :bleachProfile @00:06:30.50 PCR program should complete in < 2:45 @00:06:30.51 PCR.run 10:00 @00:09:32.33 PCR.off @00:09:32.36 AFV.select :carrierSolution @00:09:32.48 delay 2 seconds @00:09:34.51 AS.seek 1ml @00:10:32.30 delay 1 second @00:10:33.32 AFV.select :holdingCoil @00:10:33.42 delay 1 second @00:10:34.44 AS.reconfigure ASfast @00:10:34.50 AS.seek 0ml @00:11:01.87 delay 3 seconds @00:11:04.89 AFV.select :carrierSolution @00:11:04.99 delay 2 seconds @00:11:07.01 AS.reconfigure ASconfig @00:11:07.07 AS.seek 1ml @00:12:04.85 delay 1 second @00:12:05.87 AFV.select :holdingCoil @00:12:05.97 delay 1 second @00:12:06.99 AS.reconfigure ASfast @00:12:07.05 AS.seek 0ml @00:12:34.57 delay 3 seconds @00:12:37.59 AFV.select :carrierSolution @00:12:37.69 delay 2 seconds @00:12:39.71 AS.reconfigure ASconfig @00:12:39.78 AS.seek 1ml @00:13:37.60 delay 1 second @00:13:38.62 AFV.select :holdingCoil @00:13:38.72 delay 1 second @00:13:39.74 AS.reconfigure ASfast @00:13:39.80 AS.seek 0ml @00:14:07.35 delay 3 seconds @00:14:10.37 AFV.select :carrierSolution @00:14:10.47 delay 2 seconds @00:14:12.49 AS.reconfigure ASconfig @00:14:12.56 AS.seek 1ml @00:15:10.32 delay 1 second @00:15:11.34 AFV.select :holdingCoil @00:15:11.44 delay 1 second @00:15:12.46 AS.reconfigure ASfast @00:15:12.52 AS.seek 0ml @00:15:39.88 delay 3 seconds @00:15:42.90 AFV.select :carrierSolution @00:15:43.00 delay 2 seconds @00:15:45.02 AS.reconfigure ASconfig @00:15:45.08 AS.seek 1ml @00:16:42.85 delay 1 second @00:16:43.87 AFV.select :holdingCoil @00:16:43.97 delay 1 second @00:16:44.99 AS.reconfigure ASnorm @00:16:45.05 AS.seek 0ml @00:17:35.77 Setting up PCR for Tt2 -- writing /var/log/esp/Tt218aug1600h.pcr @00:17:35.79 Building PCR Slug from ASV with MM3 mix, PP6 primer @00:17:35.82 Priming with carrier @00:17:35.83 ATV.dial ARV @00:17:36.46 ARV.dial :carrier @00:17:36.82 AS.seek 0.5ml @00:18:02.34 AFV.select :carrierSolution @00:18:02.46 delay 2 seconds @00:18:04.48 AS.reconfigure ASconfig @00:18:05.07 AS.seek 1ml @00:18:34.09 delay 1 second @00:18:35.11 AFV.select :holdingCoil @00:18:35.21 delay 1 second @00:18:36.23 ATV.dial :waste @00:18:37.88 AS.reconfigure ASnorm @00:18:37.95 AS.seek :empty @00:19:28.69 AFV.select :carrierSolution @00:19:28.82 delay 2 seconds @00:19:30.84 AS.reconfigure ASconfig @00:19:30.91 AS.seek 0.5ml @00:20:00.03 delay 1 second @00:20:01.05 AFV.select :holdingCoil @00:20:01.15 Building Slug @00:20:01.16 ATV.dial :ARV @00:20:02.81 delay 2 seconds @00:20:04.83 ARV.dial :air1 @00:20:05.27 delay 2 seconds @00:20:07.35 AS.reconfigure ASslow2 @00:20:07.41 AS.seek 0.506ml @00:20:13.62 delay 2 seconds @00:20:15.64 ARV.dial :MM3 @00:20:16.71 delay 2 seconds @00:20:18.73 Adding MM3 @00:20:18.83 AS.seek 0.524ml @00:20:37.10 delay 25 seconds @00:21:02.12 ARV.dial :PP6 @00:21:02.53 Adding PP6 @00:21:02.54 delay 2 seconds @00:21:04.62 AS.seek 0.53ml @00:21:10.89 delay 10 seconds @00:21:20.92 ARV.dial :air1 @00:21:21.76 adding ARV air @00:21:21.77 delay 2 seconds @00:21:23.85 AS.seek 0.536ml @00:21:30.12 delay 5 seconds @00:21:35.14 ARV.dial :carrier @00:21:35.56 delay 7 seconds @00:21:42.64 AS.seek 0.594ml @00:22:41.12 delay 5 seconds @00:22:46.14 ATV.dial :ASV @00:22:46.54 delay 2 seconds @00:22:48.61 AS.seek 0.6ml @00:22:54.84 delay 4 seconds @00:22:58.86 ATV.dial ARV @00:22:59.42 delay 2 seconds @00:23:01.44 ARV.dial :carrier @00:23:01.45 delay 2 seconds @00:23:03.53 AS.seek 0.65ml @00:23:54.15 delay 4 seconds @00:23:58.17 ATV.dial PCR @00:23:58.79 ASV.dial :waste1 @00:23:58.80 ARV.dial :air1 @00:23:59.83 Positioning Slug by 0.324ml @00:23:59.93 AS.seek 0.326ml @00:29:25.87 delay 5 seconds @00:29:30.89 ATV.dial ARV @00:29:31.76 ASV.dial :PCRlock @00:29:32.15 delay 5 seconds @00:29:37.17 Running Tt2 Profile60C300 thermocycling @00:29:37.18 PCR.program Profile60C300 @00:29:37.33 Profile60C300 should complete in < 1:45:21 @00:29:37.34 PCR.run 2:16:41.25 @01:50:16.32 PCR.off @01:50:17.84 PCR for Tt2 completed -- closed /var/log/esp/Tt218aug1600h.pcr @01:50:17.86 Full MFB Decontamination @01:50:17.89 Decon extraction column and decon AIV @01:50:17.92 SPE.hold 2:46:40,55C @01:50:17.97 ASV.dial :SPEdelivery @01:50:18.64 delay 20 seconds @01:50:38.67 AFV.select :carrierSolution @01:50:38.77 delay 2 seconds @01:50:40.79 AS.reconfigure ASconfig @01:50:40.85 AS.seek 0.5ml @01:50:51.18 delay 1 second @01:50:52.20 AFV.select :holdingCoil @01:50:52.30 ATV.dial :air1 @01:50:54.26 AS.reconfigure ASslow1 @01:50:54.32 AS.seek 0.52ml @01:50:58.69 ATV.dial :ASV @01:51:00.51 ASV.dial :SPErecovery @01:51:01.20 AS.seek 0.62ml @01:51:21.64 ATV.dial :waste @01:51:23.54 AS.reconfigure ASnorm @01:51:23.60 AS.seek :empty @01:51:55.24 AFV.select :holdingCoil @01:51:55.26 ATV.dial :waste @01:51:55.29 AS.reconfigure ASconfig @01:51:55.36 AS.seek 0ml @01:51:55.69 AFV.select :carrierSolution @01:51:55.81 delay 2 seconds @01:51:57.84 AS.seek 0.5ml @01:52:26.87 delay 1 second @01:52:27.89 AFV.select :holdingCoil @01:52:27.99 ATV.dial :air3 @01:52:28.90 AS.reconfigure ASslow1 @01:52:28.96 AS.seek 0.52ml @01:52:33.25 ATV.dial :air3 @01:52:33.35 AS.seek 0.53ml @01:52:35.58 delay 2 seconds @01:52:37.60 ATV.dial :bleach @01:52:38.09 AS.seek 0.71ml @01:53:14.58 delay 3 seconds @01:53:17.60 ATV.dial :air3 @01:53:18.19 delay 3 seconds @01:53:21.26 AS.seek 0.735ml @01:53:26.57 delay 3 seconds @01:53:29.59 ATV.dial :bleach @01:53:30.25 AS.seek 0.72ml @01:53:33.58 delay 3 seconds @01:53:36.60 ATV.dial ASV @01:53:38.22 ASV.dial :SPErecovery @01:53:38.31 AS.seek 0.365ml @01:54:49.97 ATV.dial :waste @01:54:51.76 AS.reconfigure ASnorm @01:54:51.83 AS.seek :empty @01:55:10.61 ATV.dial :air3 @01:55:11.29 AS.reconfigure ASslow1 @01:55:11.35 AS.seek 0.009ml @01:55:13.73 delay 2 seconds @01:55:15.75 ATV.dial :bleach @01:55:16.23 AS.seek 0.579ml @01:57:11.15 delay 3 seconds @01:57:14.17 ATV.dial :air3 @01:57:14.73 delay 3 seconds @01:57:17.80 AS.seek 0.604ml @01:57:23.19 delay 3 seconds @01:57:26.21 ATV.dial :bleach @01:57:26.84 AS.seek 0.589ml @01:57:30.12 delay 3 seconds @01:57:33.14 ATV.dial :ASV @01:57:34.30 ASV.dial :wasteR @01:57:34.69 AS.seek 0ml @01:59:33.45 delay 1:00 @02:00:33.47 ATV.dial ASV @02:00:33.48 AFV.select :carrierSolution @02:00:33.60 delay 2 seconds @02:00:35.62 AS.reconfigure ASconfig @02:00:35.68 AS.seek 1ml @02:01:33.45 delay 1 second @02:01:34.47 AFV.select :holdingCoil @02:01:34.57 delay 1 second @02:01:35.59 ASV.dial :wasteR @02:01:35.61 AS.reconfigure ASslow1 @02:01:35.68 AS.seek 0ml @02:04:57.08 delay 10 seconds @02:05:07.11 ATV.dial :air3 @02:05:08.61 AS.seek 0.01ml @02:05:10.92 delay 2 seconds @02:05:12.94 ATV.dial :bleach @02:05:13.58 AS.seek 0.58ml @02:07:08.55 delay 3 seconds @02:07:11.57 ATV.dial :air3 @02:07:12.14 delay 3 seconds @02:07:15.21 AS.seek 0.605ml @02:07:20.53 delay 3 seconds @02:07:23.55 ATV.dial :bleach @02:07:24.17 AS.seek 0.59ml @02:07:27.48 delay 3 seconds @02:07:30.50 ATV.dial ASV @02:07:31.55 ASV.dial :SPEdelivery @02:07:31.92 AS.seek 0ml @02:09:30.88 delay 1:00 @02:10:30.90 AFV.select :carrierSolution @02:10:31.01 delay 2 seconds @02:10:33.03 AS.reconfigure ASconfig @02:10:33.09 AS.seek 1ml @02:11:30.86 delay 1 second @02:11:31.88 AFV.select :holdingCoil @02:11:31.98 delay 1 second @02:11:33.00 ATV.dial ASV @02:11:33.01 ASV.dial :SPEdelivery @02:11:33.04 AS.reconfigure ASslow1 @02:11:33.11 AS.seek 0ml @02:14:54.49 delay 10 seconds @02:15:04.52 ATV.dial :air2 @02:15:05.36 AS.reconfigure ASslow0 @02:15:05.42 AS.seek 0.8ml @02:16:26.21 delay 3 seconds @02:16:29.23 ATV.dial :ASV @02:16:30.22 ASV.dial :SPEdelivery @02:16:30.24 AS.reconfigure ASslow1 @02:16:30.31 AS.seek 0.03ml @02:19:05.42 delay 20 seconds @02:19:25.45 ATV.dial :waste,via: :air3 @02:19:27.18 AS.reconfigure ASconfig @02:19:27.24 AS.seek :empty @02:19:29.32 SPE.coast @02:19:29.41 Removing Bleach from Mixing Coil @02:19:29.42 AFV.select :carrierSolution @02:19:29.55 delay 2 seconds @02:19:31.58 AS.seek 0.5ml @02:20:00.69 delay 1 second @02:20:01.71 AFV.select :holdingCoil @02:20:01.82 ATV.dial :ASV @02:20:03.52 ASV.dial :SPErecovery @02:20:04.16 AS.reconfigure ASslow1 @02:20:04.23 AS.seek 0.73ml @02:20:50.74 ASV.dial :wasteD @02:20:51.33 AS.reconfigure ASnorm @02:20:51.39 AS.seek 0ml @02:21:28.54 AFV.select :carrierSolution @02:21:28.66 delay 2 seconds @02:21:30.68 AS.reconfigure ASconfig @02:21:30.74 AS.seek 0.5ml @02:21:59.80 delay 1 second @02:22:00.82 AFV.select :holdingCoil @02:22:00.92 ATV.dial ASV @02:22:00.94 ASV.dial :SPErecovery @02:22:01.59 AS.reconfigure ASslow0 @02:22:01.65 AS.seek 0.3ml @02:22:22.07 ATV.dial :air2 @02:22:23.13 AS.reconfigure ASslow1 @02:22:23.19 AS.seek 0.32ml @02:22:27.58 ATV.dial ASV @02:22:28.61 AS.reconfigure ASslow0 @02:22:28.67 AS.seek 0.57ml @02:22:54.20 ASV.dial :wasteD @02:22:54.80 AS.reconfigure ASnorm @02:22:54.87 AS.seek 0ml @02:23:23.96 AFV.select :carrierSolution @02:23:24.07 delay 2 seconds @02:23:26.09 AS.reconfigure ASconfig @02:23:26.15 AS.seek 0.5ml @02:23:55.24 delay 1 second @02:23:56.26 AFV.select :holdingCoil @02:23:56.36 ATV.dial ASV @02:23:56.37 ASV.dial :SPErecovery @02:23:57.03 AS.reconfigure ASslow0 @02:23:57.09 AS.seek 0.3ml @02:24:17.54 ATV.dial :air2 @02:24:18.58 AS.reconfigure ASslow1 @02:24:18.64 AS.seek 0.32ml @02:24:22.95 ATV.dial ASV @02:24:23.99 AS.reconfigure ASslow0 @02:24:24.05 AS.seek 0.57ml @02:24:49.53 ASV.dial :wasteD @02:24:50.13 AS.reconfigure ASnorm @02:24:50.19 AS.seek 0ml @02:25:19.26 ATV.dial :air2 @02:25:20.25 AS.reconfigure ASslow0 @02:25:20.31 AS.seek 0.3ml @02:25:50.81 ATV.dial :ASV @02:25:51.79 ASV.dial :wasteD @02:25:51.81 AS.reconfigure ASconfig @02:25:51.88 AS.seek 0.05ml @02:26:06.56 ATV.dial :waste @02:26:08.41 AS.seek 0ml @02:26:11.63 ASV.dial :SPEdelivery @02:26:12.63 deconLysateINT @02:26:12.64 ATV.dial :air3 @02:26:13.31 AS.reconfigure ASslow1 @02:26:13.37 AS.seek 0.01ml @02:26:15.74 delay 2 seconds @02:26:17.76 ATV.dial :bleach @02:26:18.24 AS.seek 0.96ml @02:29:29.55 delay 3 seconds @02:29:32.57 ATV.dial :air3 @02:29:33.13 delay 3 seconds @02:29:36.20 AS.seek 0.985ml @02:29:41.50 delay 3 seconds @02:29:44.52 ATV.dial :bleach @02:29:45.13 AS.seek 0.97ml @02:29:48.47 delay 3 seconds @02:29:51.49 ATV.dial AIV @02:29:52.74 AIV.dial :coilToMFB @02:29:52.75 AWV.select :waste @02:29:52.77 AFV.select :carrierSolution @02:29:52.90 delay 2 seconds @02:29:54.92 AS.reconfigure ASconfig @02:29:54.99 AS.seek 1ml @02:29:57.04 delay 1 second @02:29:58.06 AFV.select :holdingCoil @02:29:58.16 delay 1 second @02:29:59.18 AS.reconfigure ASslow0 @02:29:59.24 AS.seek :empty @02:31:40.20 AFV.select :carrierSolution @02:31:40.32 delay 2 seconds @02:31:42.34 AS.reconfigure ASconfig @02:31:42.40 AS.seek 1ml @02:32:40.22 delay 1 second @02:32:41.24 AFV.select :holdingCoil @02:32:41.34 delay 1 second @02:32:42.36 AS.reconfigure ASslow0 @02:32:42.42 AS.seek :empty @02:34:23.32 AFV.select :carrierSolution @02:34:23.44 delay 2 seconds @02:34:25.46 AS.reconfigure ASconfig @02:34:25.52 AS.seek 1ml @02:35:23.33 delay 1 second @02:35:24.35 AFV.select :holdingCoil @02:35:24.45 delay 1 second @02:35:25.97 AS.reconfigure ASslow0 @02:35:26.05 AS.seek :empty @02:37:07.00 ATV.dial :air2 @02:37:07.68 AS.reconfigure ASslow1 @02:37:07.74 AS.seek 0.009ml @02:37:10.10 AS.reconfigure ASslow0 @02:37:10.16 AS.seek 1ml @02:38:50.09 ATV.dial AIV @02:38:50.88 AS.seek 0.02ml @02:40:29.75 ATV.dial :waste @02:40:31.40 AS.seek 0ml @02:40:33.73 AWV.select :air @02:40:33.91 Cleaning PCR @02:40:33.92 AFV.select :carrierSolution @02:40:34.04 delay 2 seconds @02:40:36.06 AS.reconfigure ASconfig @02:40:36.12 AS.seek 0.5ml @02:41:05.16 delay 1 second @02:41:06.18 AFV.select :holdingCoil @02:41:06.28 ASV.dial :waste1 @02:41:06.65 ATV.dial :air3 @02:41:07.43 AS.reconfigure ASslow1 @02:41:07.49 AS.seek 0.51ml @02:41:09.79 delay 2 seconds @02:41:11.81 ATV.dial :bleach @02:41:12.27 AS.seek 0.81ml @02:42:12.88 delay 3 seconds @02:42:15.90 ATV.dial :air3 @02:42:16.46 delay 3 seconds @02:42:19.53 AS.seek 0.835ml @02:42:24.85 delay 3 seconds @02:42:27.87 ATV.dial :bleach @02:42:28.49 AS.seek 0.82ml @02:42:31.80 delay 3 seconds @02:42:34.82 ARV.dial :waste @02:42:35.45 delay 3 seconds @02:42:38.47 ATV.dial :ARV @02:42:39.35 AS.reconfigure ASconfig @02:42:39.41 AS.seek 0.47ml @02:42:59.83 AFV.select :carrierSolution @02:42:59.95 delay 2 seconds @02:43:01.98 AS.seek 1ml @02:43:32.76 delay 1 second @02:43:33.78 AFV.select :holdingCoil @02:43:33.88 delay 1 second @02:43:34.90 AS.reconfigure ASfast @02:43:34.97 AS.seek 0ml @02:44:02.33 AFV.select :carrierSolution @02:44:02.45 delay 2 seconds @02:44:04.47 AS.reconfigure ASconfig @02:44:04.53 AS.seek 1ml @02:45:02.32 delay 1 second @02:45:03.34 AFV.select :holdingCoil @02:45:03.44 delay 1 second @02:45:04.46 AS.reconfigure ASfast @02:45:04.52 AS.seek 0ml @02:45:31.87 AFV.select :carrierSolution @02:45:31.99 delay 2 seconds @02:45:34.02 AS.reconfigure ASconfig @02:45:34.16 AS.seek 1ml @02:46:32.01 delay 1 second @02:46:33.03 AFV.select :holdingCoil @02:46:33.13 delay 1 second @02:46:34.15 AS.reconfigure ASfast @02:46:34.21 AS.seek 0ml @02:47:01.65 ARV.dial between carrier and air1 @02:47:02.18 ATV.dial :air3 @02:47:03.46 AS.reconfigure ASslow1 @02:47:03.52 AS.seek 0.01ml @02:47:05.84 delay 2 seconds @02:47:07.86 ATV.dial :bleach @02:47:08.49 AS.seek 0.81ml @02:49:49.63 delay 3 seconds @02:49:52.65 ATV.dial :air3 @02:49:53.21 delay 3 seconds @02:49:56.28 AS.seek 0.835ml @02:50:01.58 delay 3 seconds @02:50:04.60 ATV.dial :bleach @02:50:05.21 AS.seek 0.82ml @02:50:08.51 delay 3 seconds @02:50:11.53 ATV.dial PCR @02:50:11.93 AS.reconfigure ASconfig @02:50:11.99 AS.seek :empty @02:50:59.45 PCR.program :bleachProfile @02:50:59.51 PCR program should complete in < 2:45 @02:50:59.53 PCR.run 10:00 @02:54:07.93 PCR.off @02:54:07.96 AFV.select :carrierSolution @02:54:08.09 delay 2 seconds @02:54:10.12 AS.seek 1ml @02:55:07.96 delay 1 second @02:55:08.98 AFV.select :holdingCoil @02:55:09.08 delay 1 second @02:55:10.10 AS.reconfigure ASfast @02:55:10.17 AS.seek 0ml @02:55:37.92 delay 3 seconds @02:55:40.94 AFV.select :carrierSolution @02:55:41.04 delay 2 seconds @02:55:43.06 AS.reconfigure ASconfig @02:55:43.12 AS.seek 1ml @02:56:40.91 delay 1 second @02:56:41.93 AFV.select :holdingCoil @02:56:42.03 delay 1 second @02:56:43.05 AS.reconfigure ASfast @02:56:43.12 AS.seek 0ml @02:57:10.53 delay 3 seconds @02:57:13.55 AFV.select :carrierSolution @02:57:13.65 delay 2 seconds @02:57:15.67 AS.reconfigure ASconfig @02:57:15.74 AS.seek 1ml @02:58:13.54 delay 1 second @02:58:14.56 AFV.select :holdingCoil @02:58:14.66 delay 1 second @02:58:15.68 AS.reconfigure ASfast @02:58:15.75 AS.seek 0ml @02:58:43.12 delay 3 seconds @02:58:46.14 AFV.select :carrierSolution @02:58:46.24 delay 2 seconds @02:58:48.26 AS.reconfigure ASconfig @02:58:48.33 AS.seek 1ml @02:59:46.11 delay 1 second @02:59:47.13 AFV.select :holdingCoil @02:59:47.23 delay 1 second @02:59:48.25 AS.reconfigure ASfast @02:59:48.31 AS.seek 0ml @03:00:15.84 delay 3 seconds @03:00:18.86 AFV.select :carrierSolution @03:00:18.96 delay 2 seconds @03:00:20.98 AS.reconfigure ASconfig @03:00:21.04 AS.seek 1ml @03:01:18.84 delay 1 second @03:01:19.86 AFV.select :holdingCoil @03:01:19.97 delay 1 second @03:01:20.99 AS.reconfigure ASnorm @03:01:21.05 AS.seek 0ml @03:02:11.77 AFV.select :carrierSolution @03:02:11.88 delay 2 seconds @03:02:13.90 AS.reconfigure ASconfig @03:02:13.96 AS.seek 1ml @03:03:11.78 delay 1 second @03:03:12.80 AFV.select :holdingCoil @03:03:12.90 delay 1 second @03:03:13.92 ATV.dial :waste @03:03:15.14 AS.reconfigure ASnorm @03:03:15.20 AS.seek 0.5ml @03:03:40.77 ATV.dial :waste2 @03:03:42.05 AS.seek 0ml @03:04:07.57 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118aug1603h.pcr @03:04:07.59 Building PCR Slug from elutionWater with MM2 mix, PP4 primer @03:04:07.61 Priming with carrier @03:04:07.63 ATV.dial ARV @03:04:08.47 ARV.dial :carrier @03:04:08.82 AS.seek 0.5ml @03:04:34.39 AFV.select :carrierSolution @03:04:34.51 delay 2 seconds @03:04:36.53 AS.reconfigure ASconfig @03:04:36.60 AS.seek 1ml @03:05:05.64 delay 1 second @03:05:06.66 AFV.select :holdingCoil @03:05:06.76 delay 1 second @03:05:07.78 ATV.dial :waste @03:05:09.26 AS.reconfigure ASnorm @03:05:09.32 AS.seek :empty @03:06:00.07 AFV.select :carrierSolution @03:06:00.19 delay 2 seconds @03:06:02.21 AS.reconfigure ASconfig @03:06:02.27 AS.seek 0.5ml @03:06:31.36 delay 1 second @03:06:32.38 AFV.select :holdingCoil @03:06:32.48 Building Slug @03:06:32.49 ATV.dial :ARV @03:06:34.14 delay 2 seconds @03:06:36.16 ARV.dial :air1 @03:06:36.57 delay 2 seconds @03:06:38.64 AS.reconfigure ASslow2 @03:06:38.70 AS.seek 0.506ml @03:06:45.00 delay 2 seconds @03:06:47.02 ARV.dial :MM2 @03:06:48.53 delay 2 seconds @03:06:50.55 Adding MM2 @03:06:50.64 AS.seek 0.524ml @03:07:08.89 delay 25 seconds @03:07:33.91 ARV.dial :PP4 @03:07:34.31 Adding PP4 @03:07:34.32 delay 2 seconds @03:07:36.40 AS.seek 0.53ml @03:07:42.61 delay 10 seconds @03:07:52.64 ARV.dial :air3 @03:07:53.04 adding ARV air @03:07:53.05 delay 2 seconds @03:07:55.64 AS.seek 0.536ml @03:08:01.93 delay 5 seconds @03:08:06.95 ARV.dial :carrier @03:08:08.23 delay 7 seconds @03:08:15.31 AS.seek 0.594ml @03:09:13.76 delay 5 seconds @03:09:18.78 ATV.dial :elutionWater @03:09:20.22 delay 2 seconds @03:09:22.42 AS.seek 0.6ml @03:09:28.81 delay 4 seconds @03:09:32.85 ATV.dial ARV @03:09:34.26 delay 2 seconds @03:09:36.28 ARV.dial :carrier @03:09:36.29 delay 2 seconds @03:09:38.37 AS.seek 0.65ml @03:10:28.90 delay 4 seconds @03:10:32.92 ATV.dial PCR @03:10:33.70 ASV.dial :waste1 @03:10:33.71 ARV.dial :air1 @03:10:34.14 Positioning Slug by 0.324ml @03:10:34.23 AS.seek 0.326ml @03:14:43.61 Session Ended @03:16:00.34 delay 5 seconds @03:16:05.36 ATV.dial ARV @03:16:06.12 ASV.dial :PCRlock @03:16:06.50 delay 5 seconds @03:16:11.52 Running Tt1 Profile60C300 thermocycling @03:16:11.53 PCR.program Profile60C300 @03:16:11.66 Profile60C300 should complete in < 1:45:21 @03:16:11.67 PCR.run 2:16:41.25 @04:36:44.83 PCR.off @04:36:46.35 PCR for Tt1 completed -- closed /var/log/esp/Tt118aug1603h.pcr @04:36:46.41 Cleaning PCR @04:36:46.42 AFV.select :carrierSolution @04:36:46.52 delay 2 seconds @04:36:48.54 AS.reconfigure ASconfig @04:36:48.59 AS.seek 0.5ml @04:36:58.87 delay 1 second @04:36:59.89 AFV.select :holdingCoil @04:36:59.99 ASV.dial :waste1 @04:37:00.39 ATV.dial :air3 @04:37:01.76 AS.reconfigure ASslow1 @04:37:01.80 AS.seek 0.51ml @04:37:04.08 delay 2 seconds @04:37:06.10 ATV.dial :bleach @04:37:06.69 AS.seek 0.81ml @04:38:07.28 delay 3 seconds @04:38:10.30 ATV.dial :air3 @04:38:10.84 delay 3 seconds @04:38:13.91 AS.seek 0.835ml @04:38:19.16 delay 3 seconds @04:38:22.18 ATV.dial :bleach @04:38:22.78 AS.seek 0.82ml @04:38:26.02 delay 3 seconds @04:38:29.04 ARV.dial :waste @04:38:29.66 delay 3 seconds @04:38:32.68 ATV.dial :ARV @04:38:33.53 AS.reconfigure ASconfig @04:38:33.58 AS.seek 0.47ml @04:38:53.94 AFV.select :carrierSolution @04:38:54.05 delay 2 seconds @04:38:56.08 AS.seek 1ml @04:39:26.84 delay 1 second @04:39:27.86 AFV.select :holdingCoil @04:39:27.96 delay 1 second @04:39:28.98 AS.reconfigure ASfast @04:39:29.03 AS.seek 0ml @04:39:56.37 AFV.select :carrierSolution @04:39:56.48 delay 2 seconds @04:39:58.50 AS.reconfigure ASconfig @04:39:58.55 AS.seek 1ml @04:40:56.31 delay 1 second @04:40:57.33 AFV.select :holdingCoil @04:40:57.43 delay 1 second @04:40:58.45 AS.reconfigure ASfast @04:40:58.50 AS.seek 0ml @04:41:25.91 AFV.select :carrierSolution @04:41:26.02 delay 2 seconds @04:41:28.04 AS.reconfigure ASconfig @04:41:28.09 AS.seek 1ml @04:42:25.83 delay 1 second @04:42:26.85 AFV.select :holdingCoil @04:42:26.95 delay 1 second @04:42:27.97 AS.reconfigure ASfast @04:42:28.02 AS.seek 0ml @04:42:55.71 ARV.dial between carrier and air1 @04:42:56.22 ATV.dial :air3 @04:42:57.47 AS.reconfigure ASslow1 @04:42:57.52 AS.seek 0.01ml @04:42:59.86 delay 2 seconds @04:43:01.88 ATV.dial :bleach @04:43:02.48 AS.seek 0.81ml @04:45:43.61 delay 3 seconds @04:45:46.63 ATV.dial :air3 @04:45:47.17 delay 3 seconds @04:45:50.24 AS.seek 0.835ml @04:45:55.56 delay 3 seconds @04:45:58.58 ATV.dial :bleach @04:45:59.19 AS.seek 0.82ml @04:46:02.41 delay 3 seconds @04:46:05.43 ATV.dial PCR @04:46:05.81 AS.reconfigure ASconfig @04:46:05.85 AS.seek :empty @04:46:53.30 PCR.program :bleachProfile @04:46:53.36 PCR program should complete in < 2:45 @04:46:53.86 PCR.run 10:00 @04:49:55.67 PCR.off @04:49:55.70 AFV.select :carrierSolution @04:49:55.80 delay 2 seconds @04:49:57.83 AS.seek 1ml @04:50:55.63 delay 1 second @04:50:56.65 AFV.select :holdingCoil @04:50:56.75 delay 1 second @04:50:57.77 AS.reconfigure ASfast @04:50:57.82 AS.seek 0ml @04:51:25.41 delay 3 seconds @04:51:28.43 AFV.select :carrierSolution @04:51:28.53 delay 2 seconds @04:51:30.55 AS.reconfigure ASconfig @04:51:30.61 AS.seek 1ml @04:52:28.35 delay 1 second @04:52:29.37 AFV.select :holdingCoil @04:52:29.47 delay 1 second @04:52:30.49 AS.reconfigure ASfast @04:52:30.54 AS.seek 0ml @04:52:58.02 delay 3 seconds @04:53:01.04 AFV.select :carrierSolution @04:53:01.14 delay 2 seconds @04:53:03.16 AS.reconfigure ASconfig @04:53:03.21 AS.seek 1ml @04:54:01.00 delay 1 second @04:54:02.02 AFV.select :holdingCoil @04:54:02.12 delay 1 second @04:54:03.14 AS.reconfigure ASfast @04:54:03.19 AS.seek 0ml @04:54:30.55 delay 3 seconds @04:54:33.57 AFV.select :carrierSolution @04:54:33.67 delay 2 seconds @04:54:35.69 AS.reconfigure ASconfig @04:54:35.75 AS.seek 1ml @04:55:33.49 delay 1 second @04:55:34.51 AFV.select :holdingCoil @04:55:34.61 delay 1 second @04:55:35.63 AS.reconfigure ASfast @04:55:35.68 AS.seek 0ml @04:56:03.18 delay 3 seconds @04:56:06.20 AFV.select :carrierSolution @04:56:06.30 delay 2 seconds @04:56:08.32 AS.reconfigure ASconfig @04:56:08.37 AS.seek 1ml @04:57:06.16 delay 1 second @04:57:07.18 AFV.select :holdingCoil @04:57:07.28 delay 1 second @04:57:08.30 AS.reconfigure ASnorm @04:57:08.35 AS.seek 0ml @04:57:59.08 Setting up PCR for IPc -- writing /var/log/esp/IPc18aug1604h.pcr @04:57:59.10 Building PCR Slug from elutionWater with MM1 mix, PP1 primer @04:57:59.11 Priming with carrier @04:57:59.12 ATV.dial ARV @04:57:59.71 ARV.dial :carrier @04:58:00.05 AS.seek 0.5ml @04:58:25.57 AFV.select :carrierSolution @04:58:25.68 delay 2 seconds @04:58:27.70 AS.reconfigure ASconfig @04:58:27.75 AS.seek 1ml @04:58:56.77 delay 1 second @04:58:57.79 AFV.select :holdingCoil @04:58:57.89 delay 1 second @04:58:58.91 ATV.dial :waste @04:59:00.55 AS.reconfigure ASnorm @04:59:00.59 AS.seek :empty @04:59:51.56 AFV.select :carrierSolution @04:59:51.67 delay 2 seconds @04:59:53.69 AS.reconfigure ASconfig @04:59:53.74 AS.seek 0.5ml @05:00:22.80 delay 1 second @05:00:23.82 AFV.select :holdingCoil @05:00:23.92 Building Slug @05:00:23.93 ATV.dial :ARV @05:00:25.55 delay 2 seconds @05:00:27.57 ARV.dial :air1 @05:00:27.97 delay 2 seconds @05:00:30.04 AS.reconfigure ASslow2 @05:00:30.08 AS.seek 0.506ml @05:00:36.33 delay 2 seconds @05:00:38.35 ARV.dial :MM1 @05:00:38.94 delay 2 seconds @05:00:40.96 Adding MM1 @05:00:41.02 AS.seek 0.524ml @05:00:59.38 delay 25 seconds @05:01:24.40 ARV.dial :PP1 @05:01:24.79 Adding PP1 @05:01:24.80 delay 2 seconds @05:01:26.87 AS.seek 0.53ml @05:01:33.18 delay 10 seconds @05:01:43.21 ARV.dial :air1 @05:01:43.58 adding ARV air @05:01:43.59 delay 2 seconds @05:01:45.66 AS.seek 0.536ml @05:01:51.80 delay 5 seconds @05:01:56.82 ARV.dial :carrier @05:01:57.22 delay 7 seconds @05:02:04.29 AS.seek 0.594ml @05:03:02.77 delay 5 seconds @05:03:08.28 ATV.dial :elutionWater @05:03:09.71 delay 2 seconds @05:03:11.78 AS.seek 0.6ml @05:03:18.19 delay 4 seconds @05:03:22.21 ATV.dial ARV @05:03:23.62 delay 2 seconds @05:03:25.64 ARV.dial :carrier @05:03:25.65 delay 2 seconds @05:03:27.72 AS.seek 0.65ml @05:04:18.29 delay 4 seconds @05:04:22.31 ATV.dial PCR @05:04:23.18 ASV.dial :waste1 @05:04:23.19 ARV.dial :air1 @05:04:23.59 Positioning Slug by 0.324ml @05:04:23.66 AS.seek 0.326ml @05:09:49.91 delay 5 seconds @05:09:54.93 ATV.dial ARV @05:09:55.79 ASV.dial :PCRlock @05:09:56.18 delay 5 seconds @05:10:01.20 Running IPc ProfileIPc thermocycling @05:10:01.21 PCR.program ProfileIPc @05:10:01.35 ProfileIPc should complete in < 1:50:49 @05:10:01.36 PCR.run 2:23:31.25 @06:32:57.49 PCR.off @06:33:00.07 PCR for IPc completed -- closed /var/log/esp/IPc18aug1604h.pcr @06:33:00.13 Cleaning PCR @06:33:00.14 AFV.select :carrierSolution @06:33:00.24 delay 2 seconds @06:33:02.26 AS.reconfigure ASconfig @06:33:02.31 AS.seek 0.5ml @06:33:12.62 delay 1 second @06:33:13.64 AFV.select :holdingCoil @06:33:13.74 ASV.dial :waste1 @06:33:14.21 ATV.dial :air3 @06:33:15.46 AS.reconfigure ASslow1 @06:33:15.51 AS.seek 0.51ml @06:33:17.79 delay 2 seconds @06:33:19.82 ATV.dial :bleach @06:33:20.43 AS.seek 0.81ml @06:34:20.99 delay 3 seconds @06:34:24.01 ATV.dial :air3 @06:34:24.54 delay 3 seconds @06:34:27.61 AS.seek 0.835ml @06:34:32.91 delay 3 seconds @06:34:35.93 ATV.dial :bleach @06:34:36.54 AS.seek 0.82ml @06:34:39.84 delay 3 seconds @06:34:42.86 ARV.dial :waste @06:34:43.46 delay 3 seconds @06:34:46.48 ATV.dial :ARV @06:34:47.34 AS.reconfigure ASconfig @06:34:47.39 AS.seek 0.47ml @06:35:07.76 AFV.select :carrierSolution @06:35:07.87 delay 2 seconds @06:35:09.90 AS.seek 1ml @06:35:40.67 delay 1 second @06:35:41.69 AFV.select :holdingCoil @06:35:41.79 delay 1 second @06:35:42.81 AS.reconfigure ASfast @06:35:42.86 AS.seek 0ml @06:36:10.28 AFV.select :carrierSolution @06:36:10.39 delay 2 seconds @06:36:12.41 AS.reconfigure ASconfig @06:36:12.46 AS.seek 1ml @06:37:10.21 delay 1 second @06:37:11.23 AFV.select :holdingCoil @06:37:11.33 delay 1 second @06:37:12.35 AS.reconfigure ASfast @06:37:12.40 AS.seek 0ml @06:37:39.98 AFV.select :carrierSolution @06:37:40.09 delay 2 seconds @06:37:42.11 AS.reconfigure ASconfig @06:37:42.16 AS.seek 1ml @06:38:39.90 delay 1 second @06:38:40.92 AFV.select :holdingCoil @06:38:41.02 delay 1 second @06:38:42.04 AS.reconfigure ASfast @06:38:42.09 AS.seek 0ml @06:39:09.70 ARV.dial between carrier and air1 @06:39:10.20 ATV.dial :air3 @06:39:11.45 AS.reconfigure ASslow1 @06:39:11.50 AS.seek 0.01ml @06:39:13.79 delay 2 seconds @06:39:15.81 ATV.dial :bleach @06:39:16.41 AS.seek 0.81ml @06:41:57.60 delay 3 seconds @06:42:00.62 ATV.dial :air3 @06:42:01.17 delay 3 seconds @06:42:04.24 AS.seek 0.835ml @06:42:10.01 delay 3 seconds @06:42:13.03 ATV.dial :bleach @06:42:13.63 AS.seek 0.82ml @06:42:16.89 delay 3 seconds @06:42:19.91 ATV.dial PCR @06:42:20.31 AS.reconfigure ASconfig @06:42:20.35 AS.seek :empty @06:43:07.81 PCR.program :bleachProfile @06:43:07.86 PCR program should complete in < 2:45 @06:43:07.87 PCR.run 10:00 @06:46:09.69 PCR.off @06:46:09.71 AFV.select :carrierSolution @06:46:09.82 delay 2 seconds @06:46:11.85 AS.seek 1ml @06:47:09.67 delay 1 second @06:47:10.69 AFV.select :holdingCoil @06:47:10.79 delay 1 second @06:47:11.81 AS.reconfigure ASfast @06:47:11.86 AS.seek 0ml @06:47:39.68 delay 3 seconds @06:47:42.70 AFV.select :carrierSolution @06:47:42.80 delay 2 seconds @06:47:44.82 AS.reconfigure ASconfig @06:47:44.88 AS.seek 1ml @06:48:42.64 delay 1 second @06:48:43.66 AFV.select :holdingCoil @06:48:43.76 delay 1 second @06:48:44.78 AS.reconfigure ASfast @06:48:44.83 AS.seek 0ml @06:49:12.37 delay 3 seconds @06:49:15.39 AFV.select :carrierSolution @06:49:15.49 delay 2 seconds @06:49:17.51 AS.reconfigure ASconfig @06:49:17.57 AS.seek 1ml @06:50:15.32 delay 1 second @06:50:16.34 AFV.select :holdingCoil @06:50:16.44 delay 1 second @06:50:17.46 AS.reconfigure ASfast @06:50:17.51 AS.seek 0ml @06:50:45.29 delay 3 seconds @06:50:48.31 AFV.select :carrierSolution @06:50:48.41 delay 2 seconds @06:50:50.43 AS.reconfigure ASconfig @06:50:50.49 AS.seek 1ml @06:51:48.25 delay 1 second @06:51:49.27 AFV.select :holdingCoil @06:51:49.37 delay 1 second @06:51:50.39 AS.reconfigure ASfast @06:51:50.44 AS.seek 0ml @06:52:17.98 delay 3 seconds @06:52:21.00 AFV.select :carrierSolution @06:52:21.10 delay 2 seconds @06:52:23.12 AS.reconfigure ASconfig @06:52:23.17 AS.seek 1ml @06:53:20.93 delay 1 second @06:53:21.95 AFV.select :holdingCoil @06:53:22.05 delay 1 second @06:53:23.07 AS.reconfigure ASnorm @06:53:23.12 AS.seek 0ml @06:54:13.80 Setting up PCR for Rp -- writing /var/log/esp/Rp18aug1606h.pcr @06:54:13.82 Building PCR Slug from elutionWater with MM2 mix, PP3 primer @06:54:13.83 Priming with carrier @06:54:13.84 ATV.dial ARV @06:54:14.45 ARV.dial :carrier @06:54:14.79 AS.seek 0.5ml @06:54:40.38 AFV.select :carrierSolution @06:54:40.49 delay 2 seconds @06:54:42.51 AS.reconfigure ASconfig @06:54:42.56 AS.seek 1ml @06:55:11.58 delay 1 second @06:55:12.60 AFV.select :holdingCoil @06:55:12.70 delay 1 second @06:55:13.72 ATV.dial :waste @06:55:15.36 AS.reconfigure ASnorm @06:55:15.40 AS.seek :empty @06:56:06.13 AFV.select :carrierSolution @06:56:06.24 delay 2 seconds @06:56:08.26 AS.reconfigure ASconfig @06:56:08.31 AS.seek 0.5ml @06:56:37.42 delay 1 second @06:56:38.44 AFV.select :holdingCoil @06:56:38.54 Building Slug @06:56:38.55 ATV.dial :ARV @06:56:40.17 delay 2 seconds @06:56:42.19 ARV.dial :air1 @06:56:42.59 delay 2 seconds @06:56:44.66 AS.reconfigure ASslow2 @06:56:44.70 AS.seek 0.506ml @06:56:51.18 delay 2 seconds @06:56:53.20 ARV.dial :MM2 @06:56:54.70 delay 2 seconds @06:56:56.72 Adding MM2 @06:56:56.79 AS.seek 0.524ml @06:57:15.12 delay 25 seconds @06:57:40.14 ARV.dial :PP3 @06:57:40.53 Adding PP3 @06:57:40.54 delay 2 seconds @06:57:42.61 AS.seek 0.53ml @06:57:48.75 delay 10 seconds @06:57:58.78 ARV.dial :air2 @06:57:59.67 adding ARV air @06:57:59.68 delay 2 seconds @06:58:01.75 AS.seek 0.536ml @06:58:08.09 delay 5 seconds @06:58:13.11 ARV.dial :carrier @06:58:14.40 delay 7 seconds @06:58:21.48 AS.seek 0.594ml @06:59:19.98 delay 5 seconds @06:59:25.00 ATV.dial :elutionWater @06:59:26.44 delay 2 seconds @06:59:28.51 AS.seek 0.6ml @06:59:34.70 delay 4 seconds @06:59:38.72 ATV.dial ARV @06:59:40.12 delay 2 seconds @06:59:42.14 ARV.dial :carrier @06:59:42.15 delay 2 seconds @06:59:44.22 AS.seek 0.65ml @07:00:34.72 delay 4 seconds @07:00:38.74 ATV.dial PCR @07:00:39.59 ASV.dial :waste1 @07:00:39.60 ARV.dial :air1 @07:00:40.00 Positioning Slug by 0.324ml @07:00:40.07 AS.seek 0.326ml @07:06:06.16 delay 5 seconds @07:06:11.18 ATV.dial ARV @07:06:11.94 ASV.dial :PCRlock @07:06:12.31 delay 5 seconds @07:06:17.33 Running Rp Profile60C300 thermocycling @07:06:17.34 PCR.program Profile60C300 @07:06:17.47 Profile60C300 should complete in < 1:45:21 @07:06:17.48 PCR.run 2:16:41.25 @08:13:34.93 Session Begun @08:13:39.03 -> showlog 100 @08:26:59.41 PCR.off @08:27:01.02 PCR for Rp completed -- closed /var/log/esp/Rp18aug1606h.pcr @08:27:01.09 Cleaning PCR @08:27:01.10 AFV.select :carrierSolution @08:27:01.23 delay 2 seconds @08:27:03.25 AS.reconfigure ASconfig @08:27:03.34 AS.seek 0.5ml @08:27:13.64 delay 1 second @08:27:14.66 AFV.select :holdingCoil @08:27:14.76 ASV.dial :waste1 @08:27:15.14 ATV.dial :air3 @08:27:16.44 AS.reconfigure ASslow1 @08:27:16.50 AS.seek 0.51ml @08:27:18.81 delay 2 seconds @08:27:20.84 ATV.dial :bleach @08:27:21.45 AS.seek 0.81ml @08:28:22.05 delay 3 seconds @08:28:25.07 ATV.dial :air3 @08:28:25.63 delay 3 seconds @08:28:28.70 AS.seek 0.835ml @08:28:33.93 delay 3 seconds @08:28:36.96 ATV.dial :bleach @08:28:37.58 AS.seek 0.82ml @08:28:40.89 delay 3 seconds @08:28:43.91 ARV.dial :waste @08:28:44.53 delay 3 seconds @08:28:47.55 ATV.dial :ARV @08:28:48.44 AS.reconfigure ASconfig @08:28:48.51 AS.seek 0.47ml @08:29:08.91 AFV.select :carrierSolution @08:29:09.03 delay 2 seconds @08:29:11.06 AS.seek 1ml @08:29:41.83 delay 1 second @08:29:42.85 AFV.select :holdingCoil @08:29:42.95 delay 1 second @08:29:43.97 AS.reconfigure ASfast @08:29:44.03 AS.seek 0ml @08:30:11.90 AFV.select :carrierSolution @08:30:12.02 delay 2 seconds @08:30:14.04 AS.reconfigure ASconfig @08:30:14.10 AS.seek 1ml @08:31:11.89 delay 1 second @08:31:12.91 AFV.select :holdingCoil @08:31:13.01 delay 1 second @08:31:14.03 AS.reconfigure ASfast @08:31:14.09 AS.seek 0ml @08:31:41.46 AFV.select :carrierSolution @08:31:41.58 delay 2 seconds @08:31:43.60 AS.reconfigure ASconfig @08:31:43.66 AS.seek 1ml @08:32:41.42 delay 1 second @08:32:42.44 AFV.select :holdingCoil @08:32:42.54 delay 1 second @08:32:43.56 AS.reconfigure ASfast @08:32:43.62 AS.seek 0ml @08:33:11.20 ARV.dial between carrier and air1 @08:33:11.74 ATV.dial :air3 @08:33:13.05 AS.reconfigure ASslow1 @08:33:13.11 AS.seek 0.01ml @08:33:15.46 delay 2 seconds @08:33:17.48 ATV.dial :bleach @08:33:18.09 AS.seek 0.81ml @08:35:59.22 delay 3 seconds @08:36:02.24 ATV.dial :air3 @08:36:02.78 delay 3 seconds @08:36:05.85 AS.seek 0.835ml @08:36:11.19 delay 3 seconds @08:36:14.21 ATV.dial :bleach @08:36:14.83 AS.seek 0.82ml @08:36:18.10 delay 3 seconds @08:36:21.12 ATV.dial PCR @08:36:21.52 AS.reconfigure ASconfig @08:36:21.58 AS.seek :empty @08:37:09.04 PCR.program :bleachProfile @08:37:09.10 PCR program should complete in < 2:45 @08:37:09.12 PCR.run 10:00 @08:40:11.45 PCR.off @08:40:11.49 AFV.select :carrierSolution @08:40:11.60 delay 2 seconds @08:40:13.63 AS.seek 1ml @08:41:11.44 delay 1 second @08:41:12.46 AFV.select :holdingCoil @08:41:12.56 delay 1 second @08:41:13.58 AS.reconfigure ASfast @08:41:13.64 AS.seek 0ml @08:41:41.49 delay 3 seconds @08:41:44.51 AFV.select :carrierSolution @08:41:44.61 delay 2 seconds @08:41:46.63 AS.reconfigure ASconfig @08:41:46.70 AS.seek 1ml @08:42:44.46 delay 1 second @08:42:45.48 AFV.select :holdingCoil @08:42:45.58 delay 1 second @08:42:46.60 AS.reconfigure ASfast @08:42:46.66 AS.seek 0ml @08:43:14.38 delay 3 seconds @08:43:17.40 AFV.select :carrierSolution @08:43:17.50 delay 2 seconds @08:43:19.52 AS.reconfigure ASconfig @08:43:19.60 AS.seek 1ml @08:44:17.38 delay 1 second @08:44:18.40 AFV.select :holdingCoil @08:44:18.50 delay 1 second @08:44:19.52 AS.reconfigure ASfast @08:44:19.58 AS.seek 0ml @08:44:47.21 delay 3 seconds @08:44:50.23 AFV.select :carrierSolution @08:44:50.33 delay 2 seconds @08:44:52.35 AS.reconfigure ASconfig @08:44:52.41 AS.seek 1ml @08:45:50.22 delay 1 second @08:45:51.24 AFV.select :holdingCoil @08:45:51.34 delay 1 second @08:45:52.37 AS.reconfigure ASfast @08:45:52.43 AS.seek 0ml @08:46:20.11 delay 3 seconds @08:46:23.13 AFV.select :carrierSolution @08:46:23.23 delay 2 seconds @08:46:25.25 AS.reconfigure ASconfig @08:46:25.33 AS.seek 1ml @08:47:23.13 delay 1 second @08:47:24.15 AFV.select :holdingCoil @08:47:24.25 delay 1 second @08:47:25.27 AS.reconfigure ASnorm @08:47:25.33 AS.seek 0ml @08:48:16.05 Setting up PCR for Ss -- writing /var/log/esp/Ss18aug1608h.pcr @08:48:16.07 Building PCR Slug from elutionWater with MM1 mix, PP2 primer @08:48:16.10 Priming with carrier @08:48:16.12 ATV.dial ARV @08:48:16.85 ARV.dial :carrier @08:48:17.21 AS.seek 0.5ml @08:48:42.76 AFV.select :carrierSolution @08:48:42.89 delay 2 seconds @08:48:44.91 AS.reconfigure ASconfig @08:48:44.98 AS.seek 1ml @08:49:14.02 delay 1 second @08:49:15.04 AFV.select :holdingCoil @08:49:15.14 delay 1 second @08:49:16.16 ATV.dial :waste @08:49:17.82 AS.reconfigure ASnorm @08:49:17.88 AS.seek :empty @08:50:08.64 AFV.select :carrierSolution @08:50:08.77 delay 2 seconds @08:50:10.79 AS.reconfigure ASconfig @08:50:10.85 AS.seek 0.5ml @08:50:39.96 delay 1 second @08:50:40.98 AFV.select :holdingCoil @08:50:41.08 Building Slug @08:50:41.09 ATV.dial :ARV @08:50:42.83 delay 2 seconds @08:50:44.85 ARV.dial :air1 @08:50:45.27 delay 2 seconds @08:50:47.34 AS.reconfigure ASslow2 @08:50:47.40 AS.seek 0.506ml @08:50:53.60 delay 2 seconds @08:50:55.62 ARV.dial :MM1 @08:50:56.22 delay 2 seconds @08:50:58.24 Adding MM1 @08:50:58.32 AS.seek 0.524ml @08:51:16.60 delay 25 seconds @08:51:41.62 ARV.dial :PP2 @08:51:42.02 Adding PP2 @08:51:42.03 delay 2 seconds @08:51:44.11 AS.seek 0.53ml @08:51:50.30 delay 10 seconds @08:52:00.33 ARV.dial :air2 @08:52:00.71 adding ARV air @08:52:00.72 delay 2 seconds @08:52:02.80 AS.seek 0.536ml @08:52:09.10 delay 5 seconds @08:52:14.12 ARV.dial :carrier @08:52:15.41 delay 7 seconds @08:52:22.49 AS.seek 0.594ml @08:53:21.21 delay 5 seconds @08:53:26.23 ATV.dial :elutionWater @08:53:27.66 delay 2 seconds @08:53:29.73 AS.seek 0.6ml @08:53:36.15 delay 4 seconds @08:53:40.17 ATV.dial ARV @08:53:41.61 delay 2 seconds @08:53:43.63 ARV.dial :carrier @08:53:43.64 delay 2 seconds @08:53:45.72 AS.seek 0.65ml @08:54:36.77 delay 4 seconds @08:54:40.79 ATV.dial PCR @08:54:41.55 ASV.dial :waste1 @08:54:41.56 ARV.dial :air1 @08:54:41.99 Positioning Slug by 0.324ml @08:54:42.09 AS.seek 0.326ml @09:00:07.99 delay 5 seconds @09:00:13.01 ATV.dial ARV @09:00:13.79 ASV.dial :PCRlock @09:00:14.20 delay 5 seconds @09:00:19.22 Running Ss Profile60C350 thermocycling @09:00:19.23 PCR.program Profile60C350 @09:00:19.39 Profile60C350 should complete in < 1:48:05 @09:00:19.40 PCR.run 2:20:06.25 @09:58:26.58 Session Ended @10:21:21.12 PCR.off @10:21:23.57 PCR for Ss completed -- closed /var/log/esp/Ss18aug1608h.pcr @10:21:23.63 Cleaning PCR @10:21:23.64 AFV.select :carrierSolution @10:21:23.74 delay 2 seconds @10:21:25.76 AS.reconfigure ASconfig @10:21:25.81 AS.seek 0.5ml @10:21:36.13 delay 1 second @10:21:37.15 AFV.select :holdingCoil @10:21:37.25 ASV.dial :waste1 @10:21:37.63 ATV.dial :air3 @10:21:38.90 AS.reconfigure ASslow1 @10:21:38.95 AS.seek 0.51ml @10:21:41.24 delay 2 seconds @10:21:43.27 ATV.dial :bleach @10:21:43.88 AS.seek 0.81ml @10:22:44.46 delay 3 seconds @10:22:47.48 ATV.dial :air3 @10:22:48.02 delay 3 seconds @10:22:51.09 AS.seek 0.835ml @10:22:56.32 delay 3 seconds @10:22:59.34 ATV.dial :bleach @10:22:59.94 AS.seek 0.82ml @10:23:03.23 delay 3 seconds @10:23:06.25 ARV.dial :waste @10:23:06.85 delay 3 seconds @10:23:09.87 ATV.dial :ARV @10:23:10.75 AS.reconfigure ASconfig @10:23:10.80 AS.seek 0.47ml @10:23:31.20 AFV.select :carrierSolution @10:23:31.31 delay 2 seconds @10:23:33.34 AS.seek 1ml @10:24:04.06 delay 1 second @10:24:05.08 AFV.select :holdingCoil @10:24:05.18 delay 1 second @10:24:06.20 AS.reconfigure ASfast @10:24:06.25 AS.seek 0ml @10:24:33.92 AFV.select :carrierSolution @10:24:34.03 delay 2 seconds @10:24:36.05 AS.reconfigure ASconfig @10:24:36.10 AS.seek 1ml @10:25:33.85 delay 1 second @10:25:34.87 AFV.select :holdingCoil @10:25:34.97 delay 1 second @10:25:35.99 AS.reconfigure ASfast @10:25:36.04 AS.seek 0ml @10:26:03.67 AFV.select :carrierSolution @10:26:03.78 delay 2 seconds @10:26:05.80 AS.reconfigure ASconfig @10:26:05.85 AS.seek 1ml @10:27:03.60 delay 1 second @10:27:04.62 AFV.select :holdingCoil @10:27:04.72 delay 1 second @10:27:05.74 AS.reconfigure ASfast @10:27:05.79 AS.seek 0ml @10:27:33.27 ARV.dial between carrier and air1 @10:27:33.78 ATV.dial :air3 @10:27:35.04 AS.reconfigure ASslow1 @10:27:35.09 AS.seek 0.01ml @10:27:37.39 delay 2 seconds @10:27:39.41 ATV.dial :bleach @10:27:40.13 AS.seek 0.81ml @10:30:21.26 delay 3 seconds @10:30:24.28 ATV.dial :air3 @10:30:24.92 delay 3 seconds @10:30:27.99 AS.seek 0.835ml @10:30:33.24 delay 3 seconds @10:30:36.26 ATV.dial :bleach @10:30:36.85 AS.seek 0.82ml @10:30:40.14 delay 3 seconds @10:30:43.16 ATV.dial PCR @10:30:43.57 AS.reconfigure ASconfig @10:30:43.61 AS.seek :empty @10:31:31.08 PCR.program :bleachProfile @10:31:31.13 PCR program should complete in < 2:45 @10:31:31.14 PCR.run 10:00 @10:34:32.97 PCR.off @10:34:33.00 AFV.select :carrierSolution @10:34:33.10 delay 2 seconds @10:34:35.13 AS.seek 1ml @10:35:33.45 delay 1 second @10:35:34.47 AFV.select :holdingCoil @10:35:34.57 delay 1 second @10:35:35.59 AS.reconfigure ASfast @10:35:35.64 AS.seek 0ml @10:36:03.26 delay 3 seconds @10:36:06.28 AFV.select :carrierSolution @10:36:06.38 delay 2 seconds @10:36:08.40 AS.reconfigure ASconfig @10:36:08.45 AS.seek 1ml @10:37:06.21 delay 1 second @10:37:07.23 AFV.select :holdingCoil @10:37:07.33 delay 1 second @10:37:08.35 AS.reconfigure ASfast @10:37:08.40 AS.seek 0ml @10:37:36.12 delay 3 seconds @10:37:39.14 AFV.select :carrierSolution @10:37:39.24 delay 2 seconds @10:37:41.26 AS.reconfigure ASconfig @10:37:41.32 AS.seek 1ml @10:38:39.07 delay 1 second @10:38:40.09 AFV.select :holdingCoil @10:38:40.19 delay 1 second @10:38:41.21 AS.reconfigure ASfast @10:38:41.26 AS.seek 0ml @10:39:09.12 delay 3 seconds @10:39:12.14 AFV.select :carrierSolution @10:39:12.24 delay 2 seconds @10:39:14.26 AS.reconfigure ASconfig @10:39:14.31 AS.seek 1ml @10:40:12.06 delay 1 second @10:40:13.08 AFV.select :holdingCoil @10:40:13.18 delay 1 second @10:40:14.20 AS.reconfigure ASfast @10:40:14.25 AS.seek 0ml @10:40:42.00 delay 3 seconds @10:40:45.02 AFV.select :carrierSolution @10:40:45.12 delay 2 seconds @10:40:47.14 AS.reconfigure ASconfig @10:40:47.20 AS.seek 1ml @10:41:44.95 delay 1 second @10:41:45.97 AFV.select :holdingCoil @10:41:46.07 delay 1 second @10:41:47.09 AS.reconfigure ASnorm @10:41:47.14 AS.seek 0ml @10:42:37.84 Setting up PCR for Bb -- writing /var/log/esp/Bb18aug1610h.pcr @10:42:37.86 Building PCR Slug from elutionWater with MM3 mix, PP5 primer @10:42:37.87 Priming with carrier @10:42:37.88 ATV.dial ARV @10:42:38.48 ARV.dial :carrier @10:42:38.82 AS.seek 0.5ml @10:43:04.38 AFV.select :carrierSolution @10:43:04.49 delay 2 seconds @10:43:06.51 AS.reconfigure ASconfig @10:43:06.56 AS.seek 1ml @10:43:35.58 delay 1 second @10:43:36.60 AFV.select :holdingCoil @10:43:36.70 delay 1 second @10:43:37.72 ATV.dial :waste @10:43:39.36 AS.reconfigure ASnorm @10:43:39.40 AS.seek :empty @10:44:30.13 AFV.select :carrierSolution @10:44:30.24 delay 2 seconds @10:44:32.26 AS.reconfigure ASconfig @10:44:32.31 AS.seek 0.5ml @10:45:01.39 delay 1 second @10:45:02.41 AFV.select :holdingCoil @10:45:02.51 Building Slug @10:45:02.52 ATV.dial :ARV @10:45:04.25 delay 2 seconds @10:45:06.27 ARV.dial :air1 @10:45:06.66 delay 2 seconds @10:45:08.73 AS.reconfigure ASslow2 @10:45:08.77 AS.seek 0.506ml @10:45:15.11 delay 2 seconds @10:45:17.13 ARV.dial :MM3 @10:45:18.19 delay 2 seconds @10:45:20.21 Adding MM3 @10:45:20.28 AS.seek 0.524ml @10:45:38.84 delay 25 seconds @10:46:03.86 ARV.dial :PP5 @10:46:04.25 Adding PP5 @10:46:04.26 delay 2 seconds @10:46:06.33 AS.seek 0.53ml @10:46:12.64 delay 10 seconds @10:46:22.67 ARV.dial :air3 @10:46:23.04 adding ARV air @10:46:23.05 delay 2 seconds @10:46:25.12 AS.seek 0.536ml @10:46:31.36 delay 5 seconds @10:46:36.38 ARV.dial :carrier @10:46:37.65 delay 7 seconds @10:46:44.72 AS.seek 0.594ml @10:47:43.36 delay 5 seconds @10:47:48.38 ATV.dial :elutionWater @10:47:49.80 delay 2 seconds @10:47:51.87 AS.seek 0.6ml @10:47:58.23 delay 4 seconds @10:48:02.25 ATV.dial ARV @10:48:03.66 delay 2 seconds @10:48:05.68 ARV.dial :carrier @10:48:05.69 delay 2 seconds @10:48:07.76 AS.seek 0.65ml @10:48:58.44 delay 4 seconds @10:49:02.46 ATV.dial PCR @10:49:03.20 ASV.dial :waste1 @10:49:03.21 ARV.dial :air1 @10:49:03.62 Positioning Slug by 0.324ml @10:49:04.20 AS.seek 0.326ml @10:54:30.28 delay 5 seconds @10:54:35.30 ATV.dial ARV @10:54:36.06 ASV.dial :PCRlock @10:54:36.44 delay 5 seconds @10:54:41.46 Running Bb Profile60C300 thermocycling @10:54:41.47 PCR.program Profile60C300 @10:54:41.60 Profile60C300 should complete in < 1:45:21 @10:54:41.61 PCR.run 2:16:41.25 @11:44:25.11 Session Begun @11:44:29.80 -> showlog 100 @12:09:49.24 Session Begun @12:15:13.95 PCR.off @12:15:15.47 PCR for Bb completed -- closed /var/log/esp/Bb18aug1610h.pcr @12:15:15.57 Cleaning PCR @12:15:15.58 AFV.select :carrierSolution @12:15:15.72 delay 2 seconds @12:15:17.74 AS.reconfigure ASconfig @12:15:17.82 AS.seek 0.5ml @12:15:28.13 delay 1 second @12:15:29.15 AFV.select :holdingCoil @12:15:29.25 ASV.dial :waste1 @12:15:29.64 ATV.dial :air3 @12:15:31.45 AS.reconfigure ASslow1 @12:15:31.53 AS.seek 0.51ml @12:15:33.83 delay 2 seconds @12:15:35.86 ATV.dial :bleach @12:15:36.48 AS.seek 0.81ml @12:16:37.08 delay 3 seconds @12:16:40.10 ATV.dial :air3 @12:16:40.66 delay 3 seconds @12:16:43.73 AS.seek 0.835ml @12:16:48.97 delay 3 seconds @12:16:51.99 ATV.dial :bleach @12:16:52.61 AS.seek 0.82ml @12:16:55.91 delay 3 seconds @12:16:58.93 ARV.dial :waste @12:16:59.56 delay 3 seconds @12:17:02.58 ATV.dial :ARV @12:17:03.48 AS.reconfigure ASconfig @12:17:03.55 AS.seek 0.47ml @12:17:23.97 AFV.select :carrierSolution @12:17:24.11 delay 2 seconds @12:17:26.14 AS.seek 1ml @12:17:56.89 delay 1 second @12:17:57.91 AFV.select :holdingCoil @12:17:58.01 delay 1 second @12:17:59.04 AS.reconfigure ASfast @12:17:59.12 AS.seek 0ml @12:18:26.91 AFV.select :carrierSolution @12:18:27.05 delay 2 seconds @12:18:29.07 AS.reconfigure ASconfig @12:18:29.15 AS.seek 1ml @12:19:26.92 delay 1 second @12:19:27.94 AFV.select :holdingCoil @12:19:28.04 delay 1 second @12:19:29.07 AS.reconfigure ASfast @12:19:29.14 AS.seek 0ml @12:19:56.71 AFV.select :carrierSolution @12:19:56.85 delay 2 seconds @12:19:58.87 AS.reconfigure ASconfig @12:19:58.95 AS.seek 1ml @12:20:56.72 delay 1 second @12:20:57.74 AFV.select :holdingCoil @12:20:57.84 delay 1 second @12:20:58.86 AS.reconfigure ASfast @12:20:58.94 AS.seek 0ml @12:21:26.55 ARV.dial between carrier and air1 @12:21:27.08 ATV.dial :air3 @12:21:28.39 AS.reconfigure ASslow1 @12:21:28.46 AS.seek 0.01ml @12:21:30.78 delay 2 seconds @12:21:32.80 ATV.dial :bleach @12:21:33.44 AS.seek 0.81ml @12:24:14.58 delay 3 seconds @12:24:17.60 ATV.dial :air3 @12:24:18.16 delay 3 seconds @12:24:21.23 AS.seek 0.835ml @12:24:26.60 delay 3 seconds @12:24:29.63 ATV.dial :bleach @12:24:30.25 AS.seek 0.82ml @12:24:33.57 delay 3 seconds @12:24:36.59 ATV.dial PCR @12:24:37.02 AS.reconfigure ASconfig @12:24:37.09 AS.seek :empty @12:25:24.57 PCR.program :bleachProfile @12:25:24.65 PCR program should complete in < 2:45 @12:25:24.66 PCR.run 10:00 @12:28:26.52 PCR.off @12:28:26.55 AFV.select :carrierSolution @12:28:26.69 delay 2 seconds @12:28:28.72 AS.seek 1ml @12:29:26.55 delay 1 second @12:29:27.57 AFV.select :holdingCoil @12:29:27.67 delay 1 second @12:29:28.69 AS.reconfigure ASfast @12:29:28.77 AS.seek 0ml @12:29:56.49 delay 3 seconds @12:29:59.51 AFV.select :carrierSolution @12:29:59.61 delay 2 seconds @12:30:01.64 AS.reconfigure ASconfig @12:30:01.72 AS.seek 1ml @12:30:59.53 delay 1 second @12:31:00.55 AFV.select :holdingCoil @12:31:00.65 delay 1 second @12:31:01.67 AS.reconfigure ASfast @12:31:01.75 AS.seek 0ml @12:31:29.44 delay 3 seconds @12:31:32.46 AFV.select :carrierSolution @12:31:32.56 delay 2 seconds @12:31:34.58 AS.reconfigure ASconfig @12:31:34.66 AS.seek 1ml @12:32:32.46 delay 1 second @12:32:33.48 AFV.select :holdingCoil @12:32:33.58 delay 1 second @12:32:35.05 AS.reconfigure ASfast @12:32:35.19 AS.seek 0ml @12:33:02.77 delay 3 seconds @12:33:05.79 AFV.select :carrierSolution @12:33:05.89 delay 2 seconds @12:33:07.91 AS.reconfigure ASconfig @12:33:08.00 AS.seek 1ml @12:34:05.80 delay 1 second @12:34:06.82 AFV.select :holdingCoil @12:34:06.92 delay 1 second @12:34:07.95 AS.reconfigure ASfast @12:34:08.02 AS.seek 0ml @12:34:35.50 delay 3 seconds @12:34:38.52 AFV.select :carrierSolution @12:34:38.62 delay 2 seconds @12:34:40.64 AS.reconfigure ASconfig @12:34:40.73 AS.seek 1ml @12:35:38.50 delay 1 second @12:35:39.52 AFV.select :holdingCoil @12:35:39.62 delay 1 second @12:35:40.64 AS.reconfigure ASnorm @12:35:40.71 AS.seek 0ml @12:36:31.45 Setting up PCR for Tt2 -- writing /var/log/esp/Tt218aug1612h.pcr @12:36:31.46 Building PCR Slug from elutionWater with MM3 mix, PP6 primer @12:36:31.50 Priming with carrier @12:36:31.53 ATV.dial ARV @12:36:32.16 ARV.dial :carrier @12:36:32.52 AS.seek 0.5ml @12:36:58.09 AFV.select :carrierSolution @12:36:58.23 delay 2 seconds @12:37:00.25 AS.reconfigure ASconfig @12:37:00.33 AS.seek 1ml @12:37:29.40 delay 1 second @12:37:30.42 AFV.select :holdingCoil @12:37:30.52 delay 1 second @12:37:31.54 ATV.dial :waste @12:37:33.32 AS.reconfigure ASnorm @12:37:33.39 AS.seek :empty @12:38:24.14 AFV.select :carrierSolution @12:38:24.28 delay 2 seconds @12:38:26.30 AS.reconfigure ASconfig @12:38:26.38 AS.seek 0.5ml @12:38:55.51 delay 1 second @12:38:56.53 AFV.select :holdingCoil @12:38:56.64 Building Slug @12:38:56.65 ATV.dial :ARV @12:38:58.30 delay 2 seconds @12:39:00.32 ARV.dial :air1 @12:39:00.73 delay 2 seconds @12:39:02.80 AS.reconfigure ASslow2 @12:39:02.87 AS.seek 0.506ml @12:39:09.10 delay 2 seconds @12:39:11.12 ARV.dial :MM3 @12:39:12.19 delay 2 seconds @12:39:14.21 Adding MM3 @12:39:14.32 AS.seek 0.524ml @12:39:32.57 delay 25 seconds @12:39:57.59 ARV.dial :PP6 @12:39:57.99 Adding PP6 @12:39:58.00 delay 2 seconds @12:40:00.08 AS.seek 0.53ml @12:40:06.29 delay 10 seconds @12:40:16.31 ARV.dial :air1 @12:40:17.15 adding ARV air @12:40:17.16 delay 2 seconds @12:40:19.26 AS.seek 0.536ml @12:40:25.57 delay 5 seconds @12:40:30.59 ARV.dial :carrier @12:40:31.02 delay 7 seconds @12:40:38.10 AS.seek 0.594ml @12:41:36.65 delay 5 seconds @12:41:41.67 ATV.dial :elutionWater @12:41:43.13 delay 2 seconds @12:41:45.20 AS.seek 0.6ml @12:41:51.46 delay 4 seconds @12:41:55.48 ATV.dial ARV @12:41:56.91 delay 2 seconds @12:41:58.93 ARV.dial :carrier @12:41:58.94 delay 2 seconds @12:42:01.02 AS.seek 0.65ml @12:42:51.52 delay 4 seconds @12:42:55.54 ATV.dial PCR @12:42:56.32 ASV.dial :waste1 @12:42:56.33 ARV.dial :air1 @12:42:56.78 Positioning Slug by 0.324ml @12:42:56.88 AS.seek 0.326ml @12:48:22.90 delay 5 seconds @12:48:27.92 ATV.dial ARV @12:48:28.73 ASV.dial :PCRlock @12:48:29.13 delay 5 seconds @12:48:34.15 Running Tt2 Profile60C300 thermocycling @12:48:34.17 PCR.program Profile60C300 @12:48:34.33 Profile60C300 should complete in < 1:45:21 @12:48:34.34 PCR.run 2:16:41.25 @13:33:46.38 -> exit @13:33:46.40 Session Ended @14:09:09.68 PCR.off @14:09:11.19 PCR for Tt2 completed -- closed /var/log/esp/Tt218aug1612h.pcr @14:09:11.27 Cleaning PCR @14:09:11.28 AFV.select :carrierSolution @14:09:11.41 delay 2 seconds @14:09:13.43 AS.reconfigure ASconfig @14:09:13.49 AS.seek 0.5ml @14:09:23.79 delay 1 second @14:09:24.81 AFV.select :holdingCoil @14:09:24.91 ASV.dial :waste1 @14:09:25.29 ATV.dial :air3 @14:09:26.57 AS.reconfigure ASslow1 @14:09:26.64 AS.seek 0.51ml @14:09:28.93 delay 2 seconds @14:09:30.96 ATV.dial :bleach @14:09:31.58 AS.seek 0.81ml @14:10:32.18 delay 3 seconds @14:10:35.20 ATV.dial :air3 @14:10:35.76 delay 3 seconds @14:10:38.83 AS.seek 0.835ml @14:10:44.04 delay 3 seconds @14:10:47.06 ATV.dial :bleach @14:10:47.68 AS.seek 0.82ml @14:10:51.49 delay 3 seconds @14:10:54.51 ARV.dial :waste @14:10:55.13 delay 3 seconds @14:10:58.15 ATV.dial :ARV @14:10:59.05 AS.reconfigure ASconfig @14:10:59.11 AS.seek 0.47ml @14:11:19.52 AFV.select :carrierSolution @14:11:19.64 delay 2 seconds @14:11:21.67 AS.seek 1ml @14:11:52.45 delay 1 second @14:11:53.47 AFV.select :holdingCoil @14:11:53.57 delay 1 second @14:11:54.59 AS.reconfigure ASfast @14:11:54.65 AS.seek 0ml @14:12:22.22 AFV.select :carrierSolution @14:12:22.34 delay 2 seconds @14:12:24.36 AS.reconfigure ASconfig @14:12:24.42 AS.seek 1ml @14:13:22.18 delay 1 second @14:13:23.20 AFV.select :holdingCoil @14:13:23.30 delay 1 second @14:13:24.32 AS.reconfigure ASfast @14:13:24.39 AS.seek 0ml @14:13:52.08 AFV.select :carrierSolution @14:13:52.20 delay 2 seconds @14:13:54.22 AS.reconfigure ASconfig @14:13:54.28 AS.seek 1ml @14:14:52.04 delay 1 second @14:14:53.06 AFV.select :holdingCoil @14:14:53.16 delay 1 second @14:14:54.18 AS.reconfigure ASfast @14:14:54.24 AS.seek 0ml @14:15:21.90 ARV.dial between carrier and air1 @14:15:22.40 ATV.dial :air3 @14:15:23.70 AS.reconfigure ASslow1 @14:15:23.76 AS.seek 0.01ml @14:15:26.09 delay 2 seconds @14:15:28.11 ATV.dial :bleach @14:15:28.73 AS.seek 0.81ml @14:18:09.90 delay 3 seconds @14:18:12.92 ATV.dial :air3 @14:18:13.48 delay 3 seconds @14:18:16.55 AS.seek 0.835ml @14:18:21.90 delay 3 seconds @14:18:24.92 ATV.dial :bleach @14:18:25.54 AS.seek 0.82ml @14:18:28.82 delay 3 seconds @14:18:31.84 ATV.dial PCR @14:18:32.26 AS.reconfigure ASconfig @14:18:32.32 AS.seek :empty @14:19:19.77 PCR.program :bleachProfile @14:19:19.83 PCR program should complete in < 2:45 @14:19:19.84 PCR.run 10:00 @14:22:21.68 PCR.off @14:22:21.71 AFV.select :carrierSolution @14:22:21.83 delay 2 seconds @14:22:23.86 AS.seek 1ml @14:23:21.70 delay 1 second @14:23:22.72 AFV.select :holdingCoil @14:23:22.82 delay 1 second @14:23:23.84 AS.reconfigure ASfast @14:23:23.90 AS.seek 0ml @14:23:51.72 delay 3 seconds @14:23:54.74 AFV.select :carrierSolution @14:23:54.84 delay 2 seconds @14:23:56.86 AS.reconfigure ASconfig @14:23:56.93 AS.seek 1ml @14:24:54.72 delay 1 second @14:24:55.74 AFV.select :holdingCoil @14:24:55.84 delay 1 second @14:24:56.86 AS.reconfigure ASfast @14:24:56.92 AS.seek 0ml @14:25:24.57 delay 3 seconds @14:25:27.59 AFV.select :carrierSolution @14:25:27.69 delay 2 seconds @14:25:29.71 AS.reconfigure ASconfig @14:25:29.78 AS.seek 1ml @14:26:27.56 delay 1 second @14:26:28.58 AFV.select :holdingCoil @14:26:28.68 delay 1 second @14:26:29.70 AS.reconfigure ASfast @14:26:29.76 AS.seek 0ml @14:26:57.26 delay 3 seconds @14:27:00.28 AFV.select :carrierSolution @14:27:00.38 delay 2 seconds @14:27:02.40 AS.reconfigure ASconfig @14:27:02.47 AS.seek 1ml @14:28:00.26 delay 1 second @14:28:01.28 AFV.select :holdingCoil @14:28:01.38 delay 1 second @14:28:02.40 AS.reconfigure ASfast @14:28:02.46 AS.seek 0ml @14:28:30.06 delay 3 seconds @14:28:33.08 AFV.select :carrierSolution @14:28:33.18 delay 2 seconds @14:28:35.20 AS.reconfigure ASconfig @14:28:35.27 AS.seek 1ml @14:29:33.03 delay 1 second @14:29:34.05 AFV.select :holdingCoil @14:29:34.15 delay 1 second @14:29:35.17 AS.reconfigure ASnorm @14:29:35.23 AS.seek 0ml @14:30:26.49
Asleep after completing 18tun assays @14:30:26.50 Mission runtime was 1 day, 1:30:16.895923 @14:30:26.53 GoodNews.email "{1 => 3, 2 => 0, 3 => 0, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\nCan@14:30:12, 16.5C, 47% humidity, 14.6psia, 12.060V, 0.437A, 0.437A avg, 5.27W",Subject: "Asleep after completing 18tun assays" @14:30:38.50 Session Ended @13:53:43.80CEST17-Aug-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @13:53:45.46 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @13:53:45.71 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @13:53:49.89 Polling Can every 10:00 @13:53:51.64 SPE.coast @13:53:51.69 SPE.configure SPEconfig @13:53:51.78 AS.reconfigure ASconfig @13:53:51.81 AS.hold @13:53:51.87 AS.configure ASconfig @13:53:51.95 SS.reconfigure SSconfig @13:53:51.99 SS.hold @13:53:52.04 SS.configure SSconfig @13:53:52.12 CC.reconfigure CCconfig @13:53:52.16 CC.hold @13:53:52.21 CC.configure CCconfig @13:53:52.29 CS.reconfigure CSconfig @13:53:52.33 CS.hold @13:53:52.40 CS.configure CSconfig @13:53:52.81 PC.reconfigure PCconfig @13:53:52.85 PC.hold @13:53:52.91 PC.configure PCconfig @13:53:52.99 PS.configure PSconfig @13:53:53.09 Forearm.reconfigure LooseForearm @13:53:53.13 Forearm.hold @13:53:53.18 Forearm.configure LooseForearm @13:53:53.26 Elbow.reconfigure LooseElbow @13:53:53.29 Elbow.hold @13:53:53.35 Elbow.configure LooseElbow @13:53:53.45 SC.reconfigure SCloose @13:53:53.49 SC.hold @13:53:53.54 SC.configure SCloose @13:53:53.62 SE.reconfigure SEconfig @13:53:53.66 SE.hold @13:53:53.71 SE.configure SEconfig @13:53:53.75 CH.coast @13:53:53.79 CH.configure CHconfig @13:53:53.82 PH.coast @13:53:53.87 PH.configure PHconfig @13:53:53.90 PCR.off @13:53:53.93 PCR.pidtemp= 50,0.1,0,300,25,25 @13:53:53.96 PCR.caltemp= 2.7073,0.0373,0 @13:53:53.99 All dwarf controllers running firmware version 3.75 @13:53:54.10 Cleanly exited at 13:51:12CEST17-Aug-18 @13:53:54.13 Loading /home/esp/esp2/mission/18tun.rb mission @13:53:54.20 Preparing to sleep until +0:00:15 @13:53:54.21 GoodNews.email "Starting 18tun w/pucks in tube 2\n{4 => 0, 5 => 0, 6 => 0, 7 => 0}\nCan@13:53:49, 16.2C, 53% humidity, 14.6psia, 12.016V, 0.632A, 0.626A avg, 7.59W",Subject: "18tun begun" @13:53:54.28 AS.coast @13:53:54.30
Asleep til +0:00:15 @13:53:54.34 Continuing at 13:54 @13:53:54.36 SS.coast @13:53:54.46 CC.coast @13:53:54.49 CS.coast @13:53:54.52 PC.coast @13:53:54.54 Forearm.coast @13:53:54.57 Elbow.coast @13:53:54.60 SC.coast @13:53:54.63 SE.coast @13:53:55.02 Gate.power :core,:OFF @13:53:55.04 Gate.power :raw,:OFF @13:53:55.05 Shutdown @13:54:03.17 GoodNews.email "Then, in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:106\n{4 => 0, 5 => 0, 6 => 0, 7 => 0}\nCan@13:53:54, 16.1C, 53% humidity, 14.6psia, 12.016V, 0.632A, 0.632A avg, 7.59W",Subject: "Asleep until 13:54" @13:54:09.17
Powering core bus @13:54:14.05 Gate.power :raw,:ON @13:54:14.63 SPE.coast @13:54:14.68 SPE.configure SPEconfig @13:54:14.76 AS.configure ASconfig @13:54:14.85 SS.configure SSconfig @13:54:14.94 CC.configure CCconfig @13:54:15.02 CS.configure CSconfig @13:54:15.11 PC.configure PCconfig @13:54:15.20 PS.configure PSconfig @13:54:15.30 Forearm.configure LooseForearm @13:54:15.39 Elbow.configure LooseElbow @13:54:15.49 SC.configure SCloose @13:54:15.57 SE.configure SEconfig @13:54:15.61 CH.coast @13:54:15.66 CH.configure CHconfig @13:54:15.69 PH.coast @13:54:15.74 PH.configure PHconfig @13:54:15.77 PCR.off @13:54:15.80 PCR.pidtemp= 50,0.1,0,300,25,25 @13:54:15.83 PCR.caltemp= 2.7073,0.0373,0 @13:54:15.86 All dwarf controllers running firmware version 3.75 @13:54:15.97 Valve.reset @13:54:16.15 PCR.off @13:54:16.18 PCR.pidtemp= 50,0.1,0,300,25,25 @13:54:16.21 PCR.caltemp= 2.7073,0.0373,0 @13:54:16.34 Intake.select :close @13:54:16.38 Exhaust.select :close @13:54:16.42 CTV.dial :air @13:54:16.51 PSV.dial :air @13:54:16.61 CSV.dial :waste1 @13:54:16.72 PS.reconfigure PSconfig @13:54:16.74
Forearm.reconfigure LooseForearm @13:54:16.82 PS.home.seek 0ml @13:54:16.88
Forearm.home.seek :retract @13:54:16.96 SSV.dial :spit,avoiding: :exhaust @13:54:17.44 CS.reconfigure CSfast @13:54:17.47 SS.reconfigure SSconfig @13:54:17.55 CS.home.seek 0ml @13:54:17.58 SS.home.seek 0ml @13:54:17.69 Hand.close @13:54:17.72
Elbow.jog 1500 @13:54:18.25 Elbow.reconfigure LooseElbow @13:54:18.29 Elbow.home.seek :clear @13:54:22.98 SE.reconfigure SEconfig @13:54:23.02 SE.home.seek :down @13:54:23.63 SC.reconfigure SCsloppy @13:54:23.67 SC.home.seek :initial @13:54:26.19 PS.coast @13:54:33.99
Forearm.seek :retract @13:54:34.05 Elbow.seek :hammer @13:54:35.24 Forearm.reconfigure TightForearm @13:54:35.28 Forearm.seek 5 + 350 counts @13:54:36.34 Forearm.seek :retract @13:54:37.49 PC.reconfigure PCconfig @13:54:37.50
CC.reconfigure CCconfig @13:54:37.59 CC.home.seek :open @13:54:37.61 PC.home.seek :open @13:54:40.90
Phase [coreNEG] @13:54:40.91 GoodNews.email "At 13:54:40 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:106\n{4 => 0, 5 => 0, 6 => 0, 7 => 0}\nCan@13:54:40, 16.1C, 51% humidity, 14.6psia, 12.016V, 0.632A, 0.626A avg, 7.59W",Subject: "Started 18tun phase coreNEG" @13:54:40.93CEST17-Aug-18
==================> STARTED coreNEG (phase #1) <=== @13:54:40.95 Starting initialPurge @13:54:40.96 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:54:41.02 Hand.open @13:54:41.95 Elbow.reconfigure TightElbow @13:54:41.99 Elbow.seek FlushPuck::Garage @13:54:44.07 Elbow.reconfigure LooseElbow @13:54:44.26 Forearm.reconfigure LooseForearm @13:54:44.34 Forearm.seek :garage @13:54:44.64 Session Begun @13:54:45.47
Hand.close @13:54:46.44 Forearm.seek :retract @13:54:47.48 Elbow.reconfigure TightElbow @13:54:47.56 Elbow.seek CC @13:54:50.47 Elbow.reconfigure LooseElbow @13:54:50.58 Forearm.reconfigure TightForearm @13:54:50.65 Forearm.seek CC @13:54:51.91 CC.seek :closed @13:54:54.82 -> showlog 100 @13:54:56.25
Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.29 @13:54:56.34 Hand.open @13:54:57.12 Forearm.reconfigure LooseForearm @13:54:57.19 Forearm.seek :retract @13:54:58.14 Flushing the Collection Valve::Manifold @13:54:58.29 CSV.dial CSR @13:54:59.87 Pulling 5ml of CSR.flush CSR.select :flush @13:55:00.02 CS.reconfigure CSconfig @13:55:00.08 CS.seek 5ml @13:55:13.96 delay 5 seconds @13:55:18.97 CSR.select :air @13:55:19.15 CS.reconfigure CSfast @13:55:19.21 CS.seek 7.5ml @13:55:26.67 CSV.dial :waste @13:55:28.19 CS.reconfigure CSconfig @13:55:28.25 CS.seek 0ml @13:55:48.63 Purging external Intake @13:55:48.76 CSV.dial :flush @13:55:49.30 CS.reconfigure CSfast @13:55:49.35 CS.seek 10ml @13:56:16.10 delay 5 seconds @13:56:21.13 CSV.dial CBV @13:56:21.16
CBV.dial CSV @13:56:22.15 CTV.dial :intake @13:56:23.03 Intake.select :open @13:56:23.16 CS.seek 0ml @13:56:50.92 Intake.select :close @13:56:51.04 CTV.dial :air @13:56:51.92 Flushing upper manifold @13:56:52.06 CSV.dial :flush @13:56:52.90 CS.seek 5ml @13:57:06.60 delay 5 seconds @13:57:11.62 CSV.dial :air @13:57:12.21 CS.seek 7ml @13:57:18.35 CSV.dial CBV @13:57:18.37
CBV.dial CSV @13:57:19.59 CTV.dial :air @13:57:19.62 CS.seek 0ml @13:57:38.48 delay 7 seconds @13:57:45.51 CS.seek 3ml @13:57:54.29 CSV.dial :waste @13:57:54.83 CS.seek 0ml @13:58:03.97 CSV.dial :flush @13:58:04.49 CS.seek 3ml @13:58:13.20 delay 5 seconds @13:58:18.22 CSV.dial :air @13:58:18.93 CS.seek 5ml @13:58:25.01 CSV.dial :air @13:58:25.04 CS.seek 0ml @13:58:38.79 delay 7 seconds @13:58:45.83 CS.seek 3ml @13:58:54.48 CSV.dial :waste @13:58:55.33 CS.seek 0ml @13:59:04.04 Garaging FlushPuck in Collection Clamp @13:59:04.13 Elbow.reconfigure TightElbow @13:59:04.19 Elbow.seek CC @13:59:04.45 Elbow.reconfigure LooseElbow @13:59:04.56 Forearm.seek CC @13:59:05.53 Hand.close @13:59:06.32 CC.seek :open @13:59:10.72 Forearm.seek :retract @13:59:11.74 Elbow.reconfigure TightElbow @13:59:11.81 Elbow.seek :garage @13:59:15.06 Elbow.reconfigure LooseElbow @13:59:15.17 Forearm.reconfigure TightForearm @13:59:15.22 Forearm.seek :garage @13:59:16.42 Hand.open @13:59:17.25 Forearm.reconfigure LooseForearm @13:59:17.31 Forearm.seek :retract @13:59:18.41 Starting mfb @13:59:18.42 pcrBAC wcr sampling at most 0ml @13:59:18.46 BACfans.email "Started mfb in tube 2 at 13:59:18CEST17-Aug-18\n{4 => 0, 5 => 0, 6 => 0, 7 => 0}\nCan@13:54:40, 16.1C, 51% humidity, 14.6psia, 12.016V, 0.632A, 0.626A avg, 7.59W",Subject: "pcrBAC wcr sampling at most 0ml" @13:59:18.53
Loading sh1 puck from tube 2 into Collection Clamp @13:59:18.66 Readying the MFB @13:59:18.70 Homing MFB Syringe @13:59:18.72 AFV.select :holdingCoil @13:59:18.97 ATV.dial :air2 @13:59:19.10
SC.reconfigure SCloose @13:59:19.34 Elbow.reconfigure TightElbow @13:59:19.40 Elbow.seek 2 @13:59:20.25 AS.reconfigure ASconfig @13:59:20.31 AS.home.seek :home @13:59:21.60
Forearm.reconfigure TightForearm @13:59:21.67 Forearm.seek 2 + 700 counts @13:59:22.75 SE.reconfigure SEpick @13:59:22.84 SE.seek beamBreak @13:59:25.88 AFV.select :carrierSolution @13:59:25.99
SE.configure SEconfig @13:59:26.10 delay 2 seconds @13:59:26.20
Elbow.seek 2 @13:59:26.39 Forearm.seek 2 + 300 counts @13:59:26.67 Forearm.reconfigure LooseForearm @13:59:26.73 Hand.close @13:59:27.46 SE.seek :down @13:59:27.68
Forearm.seek :retract @13:59:28.12 AS.seek 1ml @13:59:28.99
Elbow.seek CC @13:59:30.14 SE.coast @13:59:30.73
Elbow.reconfigure LooseElbow @13:59:30.85 Forearm.reconfigure TightForearm @13:59:30.91 Forearm.seek CC @13:59:32.18 CC.seek :closed @13:59:36.53 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.30 @13:59:36.54 Hand.open @13:59:37.38 Forearm.reconfigure LooseForearm @13:59:37.45 Forearm.seek :retract @13:59:38.44 SC.coast @13:59:38.50 skipping prime and sample collection @13:59:38.52 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @13:59:38.53 Intake.select :close @13:59:38.63 CSV.dial :waste @13:59:38.72 CS.seek 0ml @13:59:38.81 CSV.dial :air @13:59:38.87
Calibrating pressure and temperature sensors @13:59:38.91 CTV.dial :air @13:59:38.94 SSV.dial :spit,avoiding: :exhaust @13:59:38.97 SS.reconfigure SSfast @13:59:39.05 SS.seek 5ml @13:59:39.74 CS.seek 10ml @13:59:41.89 SSV.dial CBV @13:59:41.92
CBV.dial SSV @13:59:43.46 delay 3.8 seconds @13:59:47.37 SSV.dial :spit @13:59:48.91 SS.seek 0ml @13:59:51.65 SSV.dial CBV @13:59:53.16 SSV.dial CBV,avoiding: :exhaust @13:59:53.18
CBV.dial SSV @13:59:53.21 CTV.dial :air @13:59:53.24 delay 15 seconds @14:00:08.27 SSV.dial :spit,avoiding: :exhaust @14:00:09.80 CTV.dial CSV,avoiding: :intake @14:00:09.88
SS.reconfigure SSexhale @14:00:09.94 SS.seek 0ml @14:00:10.06 SSV.dial CBV,avoiding: :exhaust @14:00:11.67 CBV.dial between SSV and waste2 @14:00:12.10 CTV.dial CSV,avoiding: :air @14:00:12.13
CSV.dial CTV @14:00:12.66 CS.reconfigure CSpressurize @14:00:12.73 CS.seek 0ml @14:00:21.07 delay 1 second @14:00:22.09 AFV.select :holdingCoil @14:00:22.21 delay 1 second @14:00:23.23 ATV.dial :waste @14:00:24.46 AS.reconfigure ASnorm @14:00:24.51 AS.home.seek :empty @14:00:38.92
CSV.dial :air @14:00:39.46 CS.reconfigure CSfast @14:00:39.52 CS.seek 10ml @14:01:06.07 CSV.dial between air and CTV @14:01:06.45 CS.seek 3.405ml @14:01:17.09 ATV.dial :waste @14:01:17.11 ARV.dial between carrier and air1 @14:01:17.20 ASV.dial :waste1 @14:01:24.26 CTV.dial CSV,avoiding: :air @14:01:24.29
CSV.dial CTV @14:01:24.66 CS.reconfigure CSpressurize @14:01:24.73 CS.seek 0ml @14:01:34.23 CSV.dial :air @14:01:34.77 CS.reconfigure CSfast @14:01:34.83 CS.seek 10ml @14:02:01.20 CSV.dial between air and CTV @14:02:02.00 CS.seek 2.154ml @14:02:23.09 CTV.dial CSV,avoiding: :air @14:02:23.11
CSV.dial CTV @14:02:23.48 CS.reconfigure CSpressurize @14:02:23.54 CS.seek 0ml @14:02:25.46 BubblePoint: Intake @69.9psia, Outlet @14.9psia @14:02:25.47 CBV.dial SSV @14:02:25.87 SS.reconfigure SSconfig @14:02:25.93 SS.seek 10ml @14:02:32.06 delay 15 seconds @14:02:47.19 Evacuated: Intake @28.9psia, Outlet @29.3psia @14:02:47.20 SSV.dial :spit,avoiding: :exhaust @14:02:48.74 SS.reconfigure SSexhale @14:02:48.81 SS.seek 0ml @14:02:53.95 SS.reconfigure SSfast @14:02:54.02 SS.seek 5ml @14:02:56.69 SSV.dial CBV,avoiding: :exhaust @14:02:56.71
CBV.dial SSV @14:02:58.25 CTV.dial :air,avoiding: :intake @14:02:59.82 CSV.dial CTV @14:02:59.86
SS.seek 10ml @14:03:00.85 CSV.dial :air @14:03:01.38 CS.reconfigure CSfast @14:03:01.44 CS.seek 0ml @14:03:02.95
SSV.dial :spit,avoiding: :exhaust @14:03:04.49 SS.reconfigure SSexhale @14:03:04.56 SS.seek 0ml @14:03:06.80 CS.coast @14:03:09.57
SS.reconfigure SSfast @14:03:09.69 SS.seek 5ml @14:03:09.90 Session Begun @14:03:12.61 SSV.dial CBV,avoiding: :exhaust @14:03:12.64
CBV.dial SSV @14:03:15.19 SSV.dial :spit,avoiding: :exhaust @14:03:16.72 SS.reconfigure SSconfig @14:03:16.80 SS.seek 0ml @14:03:19.84 SS.coast @14:03:19.90 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @14:03:19.92 Intake.select :close @14:03:20.03 CSV.dial :waste @14:03:20.16 SSV.dial CBV,avoiding: :exhaust @14:03:20.18
CBV.dial SSV @14:03:20.93 CS.seek 0ml @14:03:21.02 CSV.dial :air @14:03:21.74
CTV.dial :air @14:03:21.75 delay 15 seconds @14:03:21.87 CS.seek 10ml @14:03:36.80
SSV.dial :spit,avoiding: :exhaust @14:03:38.33 CTV.dial CSV,avoiding: :intake @14:03:38.38
SS.reconfigure SSexhale @14:03:38.50 SS.seek 0ml @14:03:38.59 SSV.dial CBV,avoiding: :exhaust @14:03:48.58 CBV.dial between SSV and waste2 @14:03:49.03 CTV.dial CSV,avoiding: :air @14:03:49.06
CSV.dial CTV @14:03:49.60 CS.reconfigure CSpressurize @14:03:49.67 CS.seek 0ml @14:04:15.83 CSV.dial :air @14:04:16.39 CS.reconfigure CSfast @14:04:16.46 CS.seek 10ml @14:04:42.76 CSV.dial between air and CTV @14:04:43.15 CS.seek 3.382ml @14:05:00.93 CTV.dial CSV,avoiding: :air @14:05:00.95
CSV.dial CTV @14:05:01.34 CS.reconfigure CSpressurize @14:05:01.41 CS.seek 0ml @14:05:10.83 CSV.dial :air @14:05:11.39 CS.reconfigure CSfast @14:05:11.46 CS.seek 10ml @14:05:37.83 CSV.dial between air and CTV @14:05:38.63 CS.seek 2.141ml @14:05:59.65 CTV.dial CSV,avoiding: :air @14:05:59.67
CSV.dial CTV @14:06:00.06 CS.reconfigure CSpressurize @14:06:00.14 CS.seek 0ml @14:06:02.22 BubblePoint: Intake @70.9psia, Outlet @15.9psia CBV.dial SSV @14:06:02.64 SS.reconfigure SSconfig @14:06:02.71 SS.seek 10ml @14:06:08.87 delay 15 seconds @14:06:24.00 Evacuated: Intake @29.0psia, Outlet @29.9psia @14:06:24.01 SSV.dial :spit,avoiding: :exhaust @14:06:25.57 SS.reconfigure SSexhale @14:06:25.65 SS.seek 0ml @14:06:30.72 SS.reconfigure SSfast @14:06:30.80 SS.seek 5ml @14:06:33.91 SSV.dial CBV,avoiding: :exhaust @14:06:33.94
CBV.dial SSV @14:06:35.50 CTV.dial :air,avoiding: :intake @14:06:37.09 CSV.dial CTV @14:06:37.14
SS.seek 10ml @14:06:38.13 CSV.dial :air @14:06:38.67 CS.reconfigure CSfast @14:06:38.74 CS.seek 0ml @14:06:40.12
SSV.dial :spit,avoiding: :exhaust @14:06:41.66 SS.reconfigure SSexhale @14:06:41.74 SS.seek 0ml @14:06:43.80 CS.coast @14:06:46.80
SS.reconfigure SSfast @14:06:46.89 SS.seek 5ml @14:06:49.75 SSV.dial CBV,avoiding: :exhaust @14:06:49.77
CBV.dial SSV @14:06:52.35 SSV.dial :spit,avoiding: :exhaust @14:06:53.89 SS.reconfigure SSconfig @14:06:53.97 SS.seek 0ml @14:06:57.18 SS.coast @14:06:57.25 Lysing: Adding 1.5ml lysis @14:06:57.26 CBV.dial between CSV and waste1,avoiding: :DBV @14:06:59.00 CTV.dial CSV @14:07:00.57 CSV.dial CSR @14:07:01.48 Pulling 1ml of CSR.lysis @14:07:01.49 CSR.select :lysis @14:07:01.64 CS.reconfigure CSconfig @14:07:01.71 CS.seek 1ml @14:07:05.30 delay 5 seconds @14:07:10.32 CSR.select :air @14:07:10.52 CS.seek 6ml @14:07:24.27 delay 2 seconds @14:07:26.29 CSV.dial :waste1 @14:07:27.81 CS.reconfigure CSfast @14:07:27.88 CS.seek :empty @14:07:44.05 CSV.dial CSR @14:07:45.64 Pulling 1.5ml of CSR.lysis CSR.select :lysis @14:07:45.80 CS.reconfigure CSconfig @14:07:45.87 CS.seek 1.5ml @14:07:50.74 delay 5 seconds @14:07:55.76 CSR.select :air @14:07:55.96 CS.seek 4.5ml @14:08:04.74 delay 2 seconds @14:08:06.76 CSV.dial CTV @14:08:07.31 CS.seek 1.275ml @14:08:16.56 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @14:08:16.59 CH.seek 85C,8:00 @14:11:36.37 CH.hold 5:00,85C @14:16:36.55 Collection Puck Heater at 85C after lysing @14:16:36.57 CS.seek 0ml @14:16:40.88 CTV.dial between spare and air,avoiding: :intake @14:16:42.10 CSV.dial :air @14:16:42.64 CS.seek 5ml @14:16:56.40 CSV.dial CTV @14:16:56.93 delay 2 seconds @14:16:58.95 CSV.dial :air @14:16:59.50 delay 2 seconds @14:17:01.51 CSV.dial CTV @14:17:02.08 delay 2 seconds @14:17:04.09 CSV.dial :air @14:17:04.62 delay 2 seconds @14:17:06.65 CS.seek 0ml @14:17:20.40 Recovering Lysate into Collection syringe @14:17:20.42 CSV.dial CBV @14:17:21.62 CBV.dial CSV @14:17:22.07 CS.seek 5ml @14:17:36.06 delay 5 seconds @14:17:41.08 CTV.dial :air @14:17:41.71 CS.seek 7ml @14:17:47.81 delay 5 seconds @14:17:52.83 CSV.dial :air2 @14:17:53.36 CS.seek 10ml @14:18:02.00 CSV.dial between air and CTV,avoiding: CBV @14:18:03.36 Lysing Complete @14:18:03.45 Storing sh1 puck in Collection Clamp to tube 1 @14:18:03.63 SC.reconfigure SCtight @14:18:03.70 SC.seek 1 @14:18:06.07 SC.reconfigure SCloose @14:18:06.30 Elbow.reconfigure TightElbow @14:18:06.38 Elbow.seek 1 @14:18:08.31 Forearm.reconfigure TightForearm @14:18:08.38 Forearm.seek 1 + 700 counts @14:18:09.50 SE.reconfigure SEpick @14:18:09.59 SE.seek beamBreak @14:18:12.61 SE.configure SEconfig @14:18:13.12 SE.seek :place @14:18:13.68 Forearm.reconfigure LooseForearm @14:18:13.76 Forearm.seek :retract @14:18:14.69 Elbow.seek CC @14:18:16.42 Elbow.reconfigure LooseElbow @14:18:16.54 Forearm.seek CC @14:18:17.54 Hand.close @14:18:18.35 CC.seek :open @14:18:22.88 Forearm.seek :retract @14:18:23.87 Elbow.reconfigure TightElbow @14:18:23.94 Elbow.seek 1 @14:18:25.88 Elbow.reconfigure LimpElbow @14:18:25.96 Forearm.seek 1 @14:18:27.00 Elbow.hold @14:18:27.10 Elbow.reconfigure TightElbow @14:18:27.16 Hand.open @14:18:28.08 Forearm.force -20 @14:18:28.15 Forearm.seek 1 + 348 counts @14:18:28.46 Forearm.reconfigure TightForearm @14:18:28.53 Forearm.seek 1 + 700 counts @14:18:28.91 SE.seek :down @14:18:30.04
Forearm.reconfigure LooseForearm @14:18:30.11 Forearm.seek :retract @14:18:31.70 SE.coast @14:18:31.76
SC.coast @14:18:31.82 Tranferring lysate to PRV @14:18:31.83 CSV.dial :air @14:18:32.19 CS.seek 10ml @14:18:32.46 CSV.dial between air and CTV @14:18:32.82 AIV.dial :bypass @14:18:32.92 Beginning samplePassC @14:18:32.94 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:18:33.05 Hand.open @14:18:33.45 Elbow.seek FlushPuck::Garage @14:18:35.52 Elbow.reconfigure LooseElbow @14:18:35.64 Forearm.seek :garage @14:18:36.68 Hand.close @14:18:37.58 Forearm.seek :retract @14:18:38.64 Elbow.reconfigure TightElbow @14:18:38.71 Elbow.seek CC @14:18:41.63 Elbow.reconfigure LooseElbow @14:18:41.75 Forearm.reconfigure TightForearm @14:18:41.81 Forearm.seek CC @14:18:43.03 CC.seek :closed @14:18:47.30 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.25 @14:18:47.40 Hand.open @14:18:48.28 Forearm.reconfigure LooseForearm @14:18:48.35 Forearm.seek :retract @14:18:49.35 AWV.select :air @14:18:49.37 AIV.dial :bypass @14:18:49.41 PSV.dial PSR @14:18:50.99 Pulling 1.5ml of PSR.flush @14:18:51.00 PSR.select :flush @14:18:51.18 PS.seek 1.5ml @14:19:07.74 delay 5 seconds @14:19:12.75 PSV.dial :waste1 @14:19:13.30 PS.seek 0ml @14:19:29.67 PSV.dial PSR @14:19:30.21 PSR.select :air @14:19:30.36 PS.seek 2.5ml @14:19:57.27 PRV.dial CBV @14:19:57.29
CBV.dial PRV @14:19:58.22 PSV.dial :mixloop @14:19:59.40 CTV.dial :air,avoiding: :intake @14:19:59.42 PTV.dial :PRVmixing @14:19:59.55 PS.seek 0ml @14:20:26.42 PSV.dial :air @14:20:26.96 PS.seek 2.5ml @14:20:53.73 PSV.dial :mixloop @14:20:54.27 PS.seek 0ml @14:21:21.23 PSV.dial :air @14:21:21.77 PS.seek 2.5ml @14:21:48.58 PSV.dial :mixloop @14:21:49.12 PS.seek 0ml @14:22:16.08 Garaging FlushPuck in Collection Clamp @14:22:16.19 Elbow.reconfigure TightElbow @14:22:16.25 Elbow.seek CC @14:22:16.52 Elbow.reconfigure LooseElbow @14:22:16.64 Forearm.seek CC @14:22:17.63 Hand.close @14:22:18.35 CC.seek :open @14:22:22.74 Forearm.seek :retract @14:22:23.74 Elbow.reconfigure TightElbow @14:22:23.82 Elbow.seek :garage @14:22:26.93 Elbow.reconfigure LooseElbow @14:22:27.05 Forearm.reconfigure TightForearm @14:22:27.12 Forearm.seek :garage @14:22:28.33 Hand.open @14:22:29.15 Forearm.reconfigure LooseForearm @14:22:29.22 Forearm.seek :retract @14:22:30.31 Connecting PS to CS to transfer sample @14:22:30.32 CBV.dial AIV @14:22:30.36 AIV.dial :bypass @14:22:30.39 PSV.dial :mixloop @14:22:30.42 PTV.dial :PRVmixing @14:22:30.45 PRV.dial AIV @14:22:30.47 CTV.dial :air,avoiding: :intake @14:22:30.50 Loading lyfil puck from tube 2 into Collection Clamp @14:22:30.77 SC.reconfigure SCtight @14:22:30.84 SC.seek 2 @14:22:33.06 SC.reconfigure SCloose @14:22:33.29 Elbow.reconfigure TightElbow @14:22:33.36 Elbow.seek 2 @14:22:35.43 Forearm.reconfigure TightForearm @14:22:35.51 Forearm.seek 2 + 700 counts @14:22:37.04 SE.reconfigure SEpick @14:22:37.14 SE.seek beamBreak @14:22:40.27 SE.configure SEconfig @14:22:40.45 Elbow.seek 2 @14:22:40.66 Forearm.seek 2 + 300 counts @14:22:40.85 Forearm.reconfigure LooseForearm @14:22:40.92 Hand.close @14:22:41.86 SE.seek :down @14:22:42.07
Forearm.seek :retract @14:22:43.04 Elbow.seek CC @14:22:44.66 SE.coast @14:22:44.80
Elbow.reconfigure LooseElbow @14:22:44.92 Forearm.reconfigure TightForearm @14:22:44.99 Forearm.seek CC @14:22:46.19 CC.seek :closed @14:22:50.46 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.34 @14:22:50.48 Hand.open @14:22:51.27 Forearm.reconfigure LooseForearm @14:22:51.34 Forearm.seek :retract @14:22:52.35 SC.coast @14:22:52.41 CTV.dial CSV,avoiding: :intake @14:22:53.97 CSV.dial CTV @14:22:54.34 CS.seek 5ml @14:23:08.06 delay 1:20 @14:24:28.09 CS.seek 2.5ml @14:24:35.69 delay 40 seconds @14:25:15.73 CS.seek 0ml @14:25:23.28 delay 1:00 @14:26:23.30 PSV.dial :air @14:26:23.82 PS.seek 2.5ml @14:26:50.67 PSV.dial :mixloop @14:26:51.21 delay 3 seconds @14:26:54.22 PSV.dial :air @14:26:54.73 delay 2 seconds @14:26:56.75 PSV.dial :mixloop @14:26:57.29 delay 3 seconds @14:27:00.30 PSV.dial :waste1 @14:27:01.83 PS.seek :empty @14:27:28.80 CBV.dial :waste1 @14:27:29.34 delay 5 seconds @14:27:34.35 CBV.dial AIV @14:27:34.91 delay 10 seconds @14:27:44.94 CBV.dial :waste1 @14:27:45.49 delay 10 seconds @14:27:55.52 CBV.dial AIV @14:27:56.05 delay 10 seconds @14:28:06.09 CTV.dial CSV,avoiding: :intake @14:28:06.10 CBV.dial between AIV and waste1 @14:28:06.50 CSV.dial :air @14:28:07.05 CS.seek 10ml @14:28:33.60 CSV.dial CTV @14:28:34.15 CS.seek 0ml @14:29:01.00 CBV.dial :waste1 @14:29:01.35 delay 10 seconds @14:29:11.39 CTV.dial :air,avoiding: :intake @14:29:13.01 Storing lyfil puck in Collection Clamp to tube 1 @14:29:13.19 SC.reconfigure SCtight @14:29:13.26 SC.seek 1 @14:29:15.86 SC.reconfigure SCloose @14:29:16.09 Elbow.reconfigure TightElbow @14:29:16.16 Elbow.seek 1 @14:29:18.12 Forearm.reconfigure TightForearm @14:29:18.20 Forearm.seek 1 + 700 counts @14:29:19.25 SE.reconfigure SEpick @14:29:19.35 SE.seek beamBreak @14:29:22.54 SE.configure SEconfig @14:29:22.72 Elbow.reconfigure LimpElbow @14:29:22.79 Forearm.reconfigure LooseForearm @14:29:22.86 Forearm.seek 1 @14:29:23.18 Elbow.hold @14:29:23.27 Elbow.reconfigure TightElbow @14:29:23.39 SE.seek 0.03 Pucks below Top Plate @14:29:23.84 Forearm.force -20 @14:29:23.90 Forearm.seek 1 + 344 counts @14:29:24.21 Forearm.reconfigure TightForearm @14:29:24.28 Forearm.seek 1 + 700 counts @14:29:24.63 SE.seek 1.03 Pucks below Top Plate @14:29:25.25 Forearm.reconfigure LooseForearm @14:29:25.32 Forearm.seek :retract @14:29:26.26 Elbow.seek CC @14:29:27.93 Elbow.reconfigure LooseElbow @14:29:28.05 Forearm.seek CC @14:29:29.02 Hand.close @14:29:29.85 CC.seek :open @14:29:34.36 Forearm.seek :retract @14:29:35.33 Elbow.reconfigure TightElbow @14:29:35.41 Elbow.seek 1 @14:29:37.30 Elbow.reconfigure LimpElbow @14:29:37.38 Forearm.seek 1 @14:29:38.39 Elbow.hold @14:29:38.49 Elbow.reconfigure TightElbow @14:29:38.55 Hand.open @14:29:39.48 Forearm.force -20 @14:29:39.55 Forearm.seek 1 + 340 counts @14:29:39.85 Forearm.reconfigure TightForearm @14:29:39.93 Forearm.seek 1 + 700 counts @14:29:40.30 SE.seek :down @14:29:41.43
Forearm.reconfigure LooseForearm @14:29:41.50 Forearm.seek :retract @14:29:43.00 SE.coast @14:29:43.06
SC.coast @14:29:43.11 PSV.dial :air @14:29:44.49 PS.seek 0.499ml @14:29:50.49 delay 3 seconds @14:29:53.51 PSV.dial :mixloop @14:29:54.05 PRV.dial :air @14:29:54.60 delay 3 seconds @14:29:57.62 PRV.dial CBV @14:29:58.16 CBV.dial AIV @14:29:58.71 PRV.dial between CBV and air @14:29:59.53 PRV.dial :air @14:29:59.94 PS.reconfigure PSslow @14:30:00.02 PS.seek 0.599ml @14:30:02.52 PRV.dial :waste2 @14:30:03.39 PS.reconfigure PSconfig @14:30:03.46 PS.seek :empty @14:30:10.49 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:30:10.59 Hand.open @14:30:10.96 Elbow.seek FlushPuck::Garage @14:30:13.06 Elbow.reconfigure LooseElbow @14:30:13.18 Forearm.seek :garage @14:30:14.22 Hand.close @14:30:15.09 Forearm.seek :retract @14:30:16.14 Elbow.reconfigure TightElbow @14:30:16.21 Elbow.seek CC @14:30:19.04 Elbow.reconfigure LooseElbow @14:30:19.16 Forearm.reconfigure TightForearm @14:30:19.23 Forearm.seek CC @14:30:20.54 CC.seek :closed @14:30:24.81 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.20 @14:30:24.91 Hand.open @14:30:25.78 Forearm.reconfigure LooseForearm @14:30:25.85 Forearm.seek :retract @14:30:26.94 Clearing PML for modified lysate @14:30:26.95 CTV.dial :air,avoiding: :intake @14:30:27.00 PRV.dial :waste2 @14:30:27.03 PTV.dial :PRV,avoiding: :puck @14:30:27.89 delay 2 seconds @14:30:29.92 PTV.dial :PRVmixing,avoiding: :puck @14:30:30.77 delay 2 seconds @14:30:32.80 PSV.dial :PML @14:30:32.81 delay 2 seconds @14:30:34.84 PRV.dial :air @14:30:35.72 delay 2 seconds @14:30:37.79 PS.seek 1.499ml @14:30:54.27 delay 5 seconds @14:30:59.30 PS.seek 2.5ml @14:31:10.55 PSV.dial :air @14:31:11.09 PS.seek 0ml @14:31:38.02 mixing lysate with speDil @14:31:38.03 PSV.dial :PML @14:31:38.58 CBV.dial :PRV @14:31:38.60 PRV.dial :air @14:31:38.64 delay 2 seconds @14:31:40.66 PRV.dial :speDil @14:31:41.21 delay 2 seconds @14:31:43.30 PS.reconfigure PSslow1 @14:31:43.37 PS.seek 0.225ml @14:32:07.02 delay 5 seconds @14:32:12.04 PRV.dial CBV @14:32:12.93 delay 2 seconds @14:32:15.01 PS.seek 0.475ml @14:32:40.74 delay 5 seconds @14:32:45.76 PRV.dial :air @14:32:46.33 delay 2 seconds @14:32:48.35 PS.seek 0.965ml @14:33:38.24 delay 10 seconds @14:33:48.27 PRV.dial CBV @14:33:49.27 CTV.dial :air,avoiding: :intake @14:33:49.29 PSV.dial :air @14:33:49.84 PS.reconfigure PSconfig @14:33:49.92 PS.seek 2.5ml @14:34:06.61 delay 3 seconds @14:34:09.63 PSV.dial PTV @14:34:10.50 PTV.dial :PRV,avoiding: :puck @14:34:11.36 PS.seek 0ml @14:34:38.30 PSV.dial :air @14:34:39.17 PS.seek 2.5ml @14:35:05.80 delay 3 seconds @14:35:08.82 PSV.dial PTV @14:35:09.68 PTV.dial :PRV,avoiding: :puck @14:35:09.71 PS.seek 0ml @14:35:36.33 Pushing modified lysate into AIV Injection Loop @14:35:36.34 CBV.dial AIV @14:35:36.39 AIV.dial :bypass @14:35:36.42 PRV.dial CBV @14:35:36.45 PSV.dial :air @14:35:37.33 PS.seek 2.5ml @14:36:03.90 delay 3 seconds @14:36:06.92 PSV.dial :PML @14:36:07.44 PTV.dial :PRVmixing,avoiding: :puck @14:36:08.36 PS.reconfigure PSslow1 @14:36:08.44 PS.seek 1.74ml @14:37:25.83 delay 5 seconds @14:37:30.85 AIV.dial :sample @14:37:31.51 AWV.select :waste @14:37:31.70 PS.reconfigure PSslow2 @14:37:31.77 PS.seek 1.22ml @14:39:18.87 delay 15 seconds @14:39:33.89 AIV.dial :bypass @14:39:34.55 PS.reconfigure PSconfig @14:39:34.62 PS.seek 0ml @14:39:48.18 PSV.dial :air @14:39:48.72 PS.seek 2.5ml @14:40:15.30 PSV.dial :PML @14:40:15.85 PS.seek 0ml @14:40:42.46 AIV.dial :coilToMFB @14:40:42.48 AWV.select :air @14:40:42.68 Extracting Nucleic Acid from AIV @14:40:42.71
Beginning samplePassD @14:40:42.75 SPE.hold 2:46:40,55C @14:40:42.86 ASV.dial :SPEdelivery @14:40:42.95
AWV.select :air @14:40:42.96 AIV.dial :bypass @14:40:43.00 PSV.dial PSR @14:40:43.30 delay 20 seconds @14:40:44.33
Pulling 1.5ml of PSR.killMFB @14:40:44.34 PSR.select :killMFB @14:40:44.50 PS.seek 1.5ml @14:41:01.60 delay 5 seconds @14:41:03.33 primeLysateINT @14:41:03.34 AWV.select :air @14:41:03.38 AIV.dial :coilToMFB @14:41:03.40 ATV.dial AIV @14:41:05.33 AS.reconfigure ASslow1 @14:41:05.40 AS.seek 0.299ml @14:41:06.61
PSV.dial :waste1 @14:41:07.17 PS.seek 0ml @14:41:24.27 PSV.dial PSR @14:41:24.84 PSR.select :air @14:41:24.99 PS.seek 2.5ml @14:41:53.06 PRV.dial CBV @14:41:53.09
CBV.dial PRV @14:41:53.13 PSV.dial :mixloop @14:41:54.40 CTV.dial :air,avoiding: :intake @14:41:54.41 PTV.dial :PRVmixing @14:41:54.48 PS.seek 0ml @14:42:05.99 delay 10 seconds @14:42:16.02 ATV.dial :air2 @14:42:16.92 AS.seek 0.329ml @14:42:22.43
PSV.dial PSR @14:42:23.25 ATV.dial :waste2 @14:42:24.22
Pulling 1.5ml of PSR.flush @14:42:24.24 AS.reconfigure ASnorm @14:42:24.29
PSR.select :flush @14:42:24.41 AS.seek :empty @14:42:24.50
PS.seek 1.5ml @14:42:41.44 Lysate primed! @14:42:41.45 AFV.select :holdingCoil @14:42:41.49 ATV.dial :air2 @14:42:41.59
delay 5 seconds @14:42:42.18 AS.reconfigure ASslow1 @14:42:42.25 AS.seek 0.069ml @14:42:46.61
PSV.dial :waste1 @14:42:47.16 PS.seek 0ml @14:42:56.66 delay 3 seconds @14:42:59.68 ATV.dial :AIV @14:43:00.58 AS.seek 0.469ml @14:43:04.31
PSV.dial PSR @14:43:04.87 PSR.select :air @14:43:05.02 PS.seek 2.5ml @14:43:33.09 PRV.dial CBV @14:43:33.14
CBV.dial PRV @14:43:33.16 PSV.dial :mixloop @14:43:34.41 CTV.dial :air,avoiding: :intake @14:43:34.42 PTV.dial :PRVmixing @14:43:34.47 PS.seek 0ml @14:44:02.49 PSV.dial PSR @14:44:03.79 Pulling 1.5ml of PSR.flush @14:44:03.80 PSR.select :flush @14:44:03.97 PS.seek 1.5ml @14:44:20.91 delay 5 seconds @14:44:21.30 delay 5 seconds @14:44:25.92
PSV.dial :waste1 @14:44:26.37 Solid Phase Extraction Heater at 47.5C @14:44:26.38 ATV.dial ASV @14:44:26.49
PS.seek 0ml @14:44:26.82 ASV.dial :SPEdelivery @14:44:26.92 AS.seek 0.041ml @14:44:43.37
PSV.dial PSR @14:44:43.93 PSR.select :air @14:44:44.07 PS.seek 2.5ml @14:45:11.49 PRV.dial CBV @14:45:11.54
CBV.dial PRV @14:45:11.56 PSV.dial :mixloop @14:45:12.80 CTV.dial :air,avoiding: :intake @14:45:12.81 PTV.dial :PRVmixing @14:45:12.86 PS.seek 0ml @14:45:39.99 PSV.dial PSR @14:45:41.27 Pulling 1.5ml of PSR.flush @14:45:41.28 PSR.select :flush @14:45:41.44 PS.seek 1.5ml @14:45:53.28 delay 10 seconds @14:45:57.94
delay 5 seconds @14:46:02.95 PSV.dial :waste1 @14:46:03.31 rinseLysateLineINT @14:46:03.32 AFV.select :carrierSolution @14:46:03.46 delay 2 seconds @14:46:03.51
PS.seek 0ml @14:46:05.49 AS.reconfigure ASconfig @14:46:05.56 AS.seek 1ml @14:46:20.09
PSV.dial PSR @14:46:20.63 PSR.select :air @14:46:20.79 PS.seek 2.5ml @14:46:47.96 PRV.dial CBV @14:46:47.99
CBV.dial PRV @14:46:48.03 PSV.dial :mixloop @14:46:49.25 CTV.dial :air,avoiding: :intake @14:46:49.26 PTV.dial :PRVmixing @14:46:49.33 PS.reconfigure PSslow @14:46:49.39 PS.seek 1.2ml @14:47:00.97 delay 1 second @14:47:01.99 AFV.select :holdingCoil @14:47:02.10 delay 1 second @14:47:03.11 ATV.dial AIV @14:47:03.78 AIV.dial :coilToMFB @14:47:03.80 AWV.select :waste @14:47:03.94 AS.reconfigure ASslow0 @14:47:04.02 AS.seek 0ml @14:47:16.21
PSV.dial :waste1 @14:47:17.76 PS.reconfigure PSconfig @14:47:17.84 PS.seek 0ml @14:47:31.25 PSV.dial :air @14:47:32.46 PS.seek 2.5ml @14:47:59.84 PSV.dial :mixloop @14:48:00.36 PS.reconfigure PSslow @14:48:00.44 PS.seek 0ml @14:48:44.97 ATV.dial :air2 @14:48:45.68 AS.reconfigure ASslow1 @14:48:45.75 AS.seek 0.05ml @14:48:51.62
PSV.dial :air @14:48:52.16 PS.reconfigure PSconfig @14:48:52.23 PS.seek 2.5ml @14:48:56.11 ATV.dial :AIV @14:48:56.98 AS.seek 0.01ml @14:49:05.33 ATV.dial :waste @14:49:07.04 AS.seek :empty @14:49:09.52 ethanol wash @14:49:09.53 ATV.dial :air2 @14:49:10.84 AS.reconfigure ASslow2 @14:49:10.92 AS.seek 0.009ml @14:49:19.06
PSV.dial :mixloop @14:49:19.60 PS.reconfigure PSslow @14:49:19.67 PS.seek 0ml @14:49:21.58 delay 3 seconds @14:49:24.61 Pulling 0.4ml of Ethanol Wash @14:49:24.62 ATV.dial :ethanol @14:49:25.50 AS.reconfigure ASslow1 @14:49:25.57 AS.seek 0.409ml @14:50:10.84
Cleaning Collection Syringe @14:50:10.85 CSV.dial CSR @14:50:11.46 Pulling 4ml of CSR.flush @14:50:11.47 CSR.select :flush @14:50:11.65 CS.seek 4ml @14:50:23.06 delay 5 seconds @14:50:28.07 CSR.select :air @14:50:28.22 CS.seek 10ml @14:50:44.71 CSV.dial CTV @14:50:44.74
CTV.dial CSV @14:50:46.31 delay 3 seconds @14:50:46.37
CBV.dial :waste1 @14:50:46.91 CS.seek 0ml @14:50:49.32 ATV.dial :air1 @14:50:49.75 delay 3 seconds @14:50:52.83 AS.seek 0.434ml @14:50:58.14 delay 3 seconds @14:51:01.16 ATV.dial :ethanol @14:51:01.78 AS.seek 0.419ml @14:51:05.10 delay 3 seconds @14:51:08.12 ATV.dial ASV @14:51:09.49 AS.seek 0.092ml @14:51:13.55
CSV.dial CSR @14:51:14.14 Pulling 4ml of CSR.flush @14:51:14.15 CSR.select :flush @14:51:14.31 CS.seek 4ml @14:51:25.66 delay 5 seconds @14:51:30.67 CSR.select :air @14:51:30.81 CS.seek 10ml @14:51:47.15 CSV.dial CTV @14:51:47.19
CTV.dial CSV @14:51:47.73 CBV.dial :waste1 @14:51:47.75 CS.seek 0ml @14:52:14.33 Garaging FlushPuck in Collection Clamp @14:52:14.43 Elbow.reconfigure TightElbow @14:52:14.50 Elbow.seek CC @14:52:14.75 Elbow.reconfigure LooseElbow @14:52:14.87 Forearm.seek CC @14:52:15.54 delay 3 seconds @14:52:15.87
Hand.close @14:52:16.63 CC.seek :open @14:52:18.56 ATV.dial :waste2 @14:52:19.37 AS.reconfigure ASconfig @14:52:19.44 AS.seek :empty @14:52:21.04
Forearm.seek :retract @14:52:22.47 Elbow.reconfigure TightElbow @14:52:22.56 Elbow.seek :garage @14:52:25.11 ATV.dial :air2 @14:52:25.55 AS.reconfigure ASslow1 @14:52:25.62 AS.seek 1ml @14:52:25.88
Elbow.reconfigure LooseElbow @14:52:26.00 Forearm.reconfigure TightForearm @14:52:26.07 Forearm.seek :garage @14:52:27.29 Hand.open @14:52:28.06 Forearm.reconfigure LooseForearm @14:52:28.13 Forearm.seek :retract @14:52:29.13 Whole Cell Archive sampling 0ml @14:52:29.15 Loading wcr puck from tube 2 into Collection Clamp @14:52:29.43 SC.reconfigure SCtight @14:52:29.50 SC.seek 2 @14:52:31.73 SC.reconfigure SCloose @14:52:31.97 Elbow.reconfigure TightElbow @14:52:32.05 Elbow.seek 2 @14:52:34.28 Forearm.reconfigure TightForearm @14:52:34.36 Forearm.seek 2 + 700 counts @14:52:35.37 SE.reconfigure SEpick @14:52:35.46 SE.seek beamBreak @14:52:38.70 SE.configure SEconfig @14:52:38.88 Elbow.seek 2 @14:52:39.09 Forearm.seek 2 + 300 counts @14:52:39.35 Forearm.reconfigure LooseForearm @14:52:39.43 Hand.close @14:52:40.27 SE.seek :down @14:52:40.47 Forearm.seek :retract @14:52:41.44 Elbow.seek CC @14:52:43.06 Elbow.reconfigure LooseElbow @14:52:43.18 Forearm.reconfigure TightForearm @14:52:43.27 SE.coast @14:52:43.30 Forearm.seek CC @14:52:44.66 CC.seek :closed @14:52:48.92 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.42 @14:52:48.94 Hand.open @14:52:49.80 Forearm.reconfigure LooseForearm @14:52:49.88 Forearm.seek :retract @14:52:50.90 SC.coast @14:52:50.97 collectSample 0ml w/bubble point of 55psi @14:52:50.99 Priming 7ml @14:52:51.02 Gate.power :sampler,:ON @14:52:51.06 Intake.select :close @14:52:51.08 Exhaust.select :close @14:52:51.12 SS.hold @14:52:51.36 Calibrating pressure sensors @14:52:51.37 CTV.dial :air @14:52:53.46 SSV.dial :spit,avoiding: :exhaust @14:52:53.49 SS.reconfigure SSfast @14:52:53.58 SS.seek 5ml @14:52:56.47 SSV.dial CBV @14:52:56.50 CBV.dial SSV @14:52:58.15 delay 3.8 seconds @14:53:02.07 SSV.dial :spit @14:53:03.62 SS.seek 0ml @14:53:06.61 SSV.dial CBV @14:53:08.19 CTV.dial :intake @14:53:09.07 Waiting for external sampler to finish priming... @14:53:09.08 delayUntil "14:57:51.05" @14:53:52.02 Session Begun @14:53:56.07 -> showlog 100 @14:55:47.19 delay 5 seconds @14:55:52.21 ATV.dial ASV @14:55:53.25 ASV.dial :SPEdelivery @14:55:53.29 AS.seek 0.02ml @14:57:51.07 SSV.dial CBV @14:57:51.10 CBV.dial SSV @14:57:51.17 Allowing 40s for 7.0ml of remaining 7.0ml @14:57:51.22 Intake.select :open @14:57:53.42 Intake @29.0psia, vacuum from 20.0 to 24.0psi @14:57:53.45 SS.reconfigure SSinhale.with maxSpeed: 150 @14:57:53.57 SS.seek 7ml @14:57:59.64 Stroke completed -- waiting for pressure to equalize ... @14:58:08.25 Intake.select :close @14:58:08.43 SSV.dial :exhaust @14:58:09.33 Exhaust.select :open @14:58:09.48 SS.reconfigure SSexhale @14:58:09.57 SS.seek 0ml @14:58:13.68 Exhaust.select :close @14:58:13.81 Sampling 0.0ml @14:58:13.85 Intake.select :close @14:58:13.87 Exhaust.select :close @14:58:13.89 SS.reconfigure SSconfig @14:58:14.02 SS.hold @14:58:14.25 CTV.dial :intake @14:58:14.31 Sampled 0.0ml @14:58:14.33 Drying puck before fixing for 4:00 @14:58:14.35 CBV.dial :waste2b @14:58:15.29 delay 1:00 @14:58:17.28 Gate.power :sampler,:OFF @14:59:10.69 delay 5 seconds @14:59:15.31 CSV.dial :air @14:59:15.71 ATV.dial :air2 @14:59:15.87 CS.reconfigure CSfast @14:59:15.95 CS.seek 10ml @14:59:16.80 AS.seek 0.52ml @14:59:42.75 Pumping wcr puck from tube 2 up to 55psi... @14:59:42.77 CBV.dial SSV @14:59:44.26 CBV.dial between CSV and waste2b @14:59:45.40 CSV.dial between air and CTV @14:59:45.82 CS.seek 7.397ml @14:59:53.88 CTV.dial CSV,avoiding: :air @14:59:53.91 CSV.dial CTV @14:59:54.84 CS.reconfigure CSpressurize @14:59:54.93 CS.seek 0ml @15:00:14.53 CSV.dial :air @15:00:15.21 CS.reconfigure CSfast @15:00:15.30 CS.seek 10ml @15:00:42.15 CSV.dial between air and CTV @15:00:42.55 CS.seek 2.547ml @15:00:57.69 delay 5 seconds @15:01:02.76 CTV.dial CSV,avoiding: :air @15:01:02.80 CSV.dial CTV @15:01:02.90 ATV.dial ASV @15:01:03.20 CS.reconfigure CSpressurize @15:01:03.29 CS.seek 0ml @15:01:03.91 ASV.dial :wasteR @15:01:04.41 AS.seek 0.3ml @15:01:08.09 CBV.dial :waste2b @15:01:08.51 CS.reconfigure CSconfig @15:01:08.59 CS.seek :empty @15:01:11.94 delay 1:00 @15:01:48.93 delay 20 seconds @15:02:08.97 AS.seek 0.07ml @15:02:11.97 CTV.dial :air,avoiding: :intake @15:02:13.57 Fix #1 of 2 (with rnal) for 2:00 @15:02:13.58 CSV.dial CSR @15:02:14.19 Pulling 2ml of CSR.rnal @15:02:14.20 CSR.select :rnal @15:02:14.38 CS.seek 2ml @15:02:20.75 delay 5 seconds @15:02:25.78 CSR.select :air @15:02:25.94 CS.reconfigure CSfast @15:02:26.03 CS.seek 10ml @15:02:47.44 Pumping wcr puck from tube 2 up to 55psi... @15:02:47.46 CBV.dial SSV @15:02:48.94 CBV.dial between CSV and waste2b @15:02:50.07 CSV.dial between air and CTV @15:02:50.81 CS.seek 7.238ml @15:02:55.47 delay 20 seconds @15:02:58.89 CTV.dial CSV,avoiding: :air @15:02:58.92 CSV.dial CTV @15:03:00.55 CS.reconfigure CSpressurize @15:03:00.64 CS.seek 0ml @15:03:15.49 ATV.dial :waste2 @15:03:16.31 AS.reconfigure ASconfig @15:03:16.39 AS.seek :empty @15:03:19.88 CSV.dial :air @15:03:20.45 CS.reconfigure CSfast @15:03:20.55 CS.seek 10ml @15:03:20.79 Elution @15:03:20.80 ATV.dial :air1 @15:03:21.97 AS.reconfigure ASslow1 @15:03:22.06 AS.seek 0.049ml @15:03:32.46 delay 3 seconds @15:03:35.48 ATV.dial :ElutionWater @15:03:36.20 AS.seek 0.109ml @15:03:47.15 CSV.dial between air and CTV @15:03:48.03 CS.seek 2.17ml @15:03:48.54 delay 3 seconds @15:03:51.57 ATV.dial ASV,via: :air2 @15:03:53.73 ASV.dial :SPEelution @15:03:54.37 AFV.select :carrierSolution @15:03:54.54 AS.reconfigure ASnorm @15:03:54.62 AS.seek 0.759ml @15:04:09.20 CTV.dial CSV,avoiding: :air @15:04:09.23 CSV.dial CTV @15:04:09.64 CS.reconfigure CSpressurize @15:04:09.73 CS.seek 0ml @15:04:11.87 delay 2:00 @15:04:27.69 delay 3 seconds @15:04:30.71 AFV.select :holdingCoil @15:04:30.90 AS.reconfigure ASslow1 @15:04:30.98 AS.seek 0.474ml @15:05:28.62 delay 5 seconds @15:05:33.71 AS.reconfigure ASslow2 @15:05:33.79 AS.seek 0.439ml @15:06:09.23 delay 1:00 @15:06:11.89 CBV.dial :waste2b @15:06:12.31 CS.reconfigure CSconfig @15:06:12.39 CS.seek :empty @15:06:17.72 delay 1:00 @15:07:09.26 pushing eluate to holding coil @15:07:09.36 AS.seek 0.279ml @15:07:17.75 CTV.dial :air,avoiding: :intake @15:07:19.31 Fix #2 of 2 (with rnal) for 2:00 @15:07:19.34 CSV.dial CSR @15:07:19.94 Pulling 2ml of CSR.rnal @15:07:19.96 CSR.select :rnal @15:07:20.14 CS.seek 2ml @15:07:26.47 delay 5 seconds @15:07:31.50 CSR.select :air @15:07:31.67 CS.reconfigure CSfast @15:07:31.76 CS.seek 10ml @15:07:53.33 Pumping wcr puck from tube 2 up to 55psi... @15:07:53.34 CBV.dial SSV @15:07:54.81 CBV.dial between CSV and waste2b @15:07:55.95 CSV.dial between air and CTV @15:07:56.70 CS.seek 7.23ml @15:08:04.89 CTV.dial CSV,avoiding: :air @15:08:04.92 CSV.dial CTV @15:08:06.55 CS.reconfigure CSpressurize @15:08:06.64 CS.seek 0ml @15:08:25.85 CSV.dial :air @15:08:26.42 CS.reconfigure CSfast @15:08:26.51 CS.seek 10ml @15:08:53.19 CSV.dial between air and CTV @15:08:53.59 CS.seek 2.155ml @15:09:14.98 CTV.dial CSV,avoiding: :air @15:09:15.01 CSV.dial CTV @15:09:15.42 CS.reconfigure CSpressurize @15:09:15.51 CS.seek 0ml @15:09:17.46 delay 2:00 @15:09:50.46 delay 30 seconds @15:10:20.50 ATV.dial :waste,via: :air3 @15:10:22.25 AS.reconfigure ASconfig @15:10:22.33 AS.seek :empty @15:10:38.71 Parking Bleach in LysateSource line and SPEcolumn @15:10:38.72 ATV.dial :air3 @15:10:39.60 AS.reconfigure ASslow1 @15:10:39.68 AS.seek 0.029ml @15:10:46.09 ATV.dial :ElutionWater @15:10:47.21 AS.seek 0.059ml @15:10:53.52 ATV.dial :air3 @15:10:54.17 AS.seek 0.069ml @15:10:56.48 delay 2 seconds @15:10:58.50 ATV.dial :bleach @15:10:59.00 AS.seek 0.769ml @15:11:17.48 CBV.dial :waste2b @15:11:17.91 CS.reconfigure CSconfig @15:11:17.99 CS.seek :empty @15:11:23.16 delay 1:00 @15:12:23.20 CTV.dial :air,avoiding: :intake @15:12:24.84 Storing wcr puck in Collection Clamp to tube 1 @15:12:25.05 SC.reconfigure SCtight @15:12:25.13 SC.seek 1 @15:12:27.49 SC.reconfigure SCloose @15:12:27.74 Elbow.reconfigure TightElbow @15:12:27.82 Elbow.seek 1 @15:12:29.76 Forearm.reconfigure TightForearm @15:12:29.85 Forearm.seek 1 + 700 counts @15:12:30.96 SE.reconfigure SEpick @15:12:31.08 SE.seek beamBreak @15:12:34.25 SE.configure SEconfig @15:12:34.44 Elbow.reconfigure LimpElbow @15:12:34.52 Forearm.reconfigure LooseForearm @15:12:34.60 Forearm.seek 1 @15:12:34.92 Elbow.hold @15:12:35.03 Elbow.reconfigure TightElbow @15:12:35.16 SE.seek 1.04 Pucks below Top Plate @15:12:35.60 Forearm.force -20 @15:12:35.68 Forearm.seek 1 + 341 counts @15:12:36.02 Forearm.reconfigure TightForearm @15:12:36.11 Forearm.seek 1 + 700 counts @15:12:36.44 SE.seek 2.04 Pucks below Top Plate @15:12:37.06 Forearm.reconfigure LooseForearm @15:12:37.14 Forearm.seek :retract @15:12:38.12 Elbow.seek CC @15:12:39.88 Elbow.reconfigure LooseElbow @15:12:40.01 Forearm.seek CC @15:12:41.03 Hand.close @15:12:41.77 CC.seek :open @15:12:46.23 Forearm.seek :retract @15:12:47.27 Elbow.reconfigure TightElbow @15:12:47.35 Elbow.seek 1 @15:12:49.37 Elbow.reconfigure LimpElbow @15:12:49.46 Forearm.seek 1 @15:12:50.99 Elbow.hold @15:12:51.12 Elbow.reconfigure TightElbow @15:12:51.19 Hand.open @15:12:52.14 Forearm.force -20 @15:12:52.21 Forearm.seek 1 + 337 counts @15:12:52.55 Forearm.reconfigure TightForearm @15:12:52.64 Forearm.seek 1 + 700 counts @15:12:53.01 SE.seek :down @15:12:54.13 Forearm.reconfigure LooseForearm @15:12:54.22 Forearm.seek :retract @15:12:55.66 SE.coast @15:12:55.73 SC.coast @15:12:55.81 Starting finalPurge @15:12:55.83 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:12:55.95 Hand.open @15:12:56.39 Elbow.seek FlushPuck::Garage @15:12:58.45 Elbow.reconfigure LooseElbow @15:12:58.59 Forearm.seek :garage @15:12:59.61 Hand.close @15:13:00.51 Forearm.seek :retract @15:13:01.56 Elbow.reconfigure TightElbow @15:13:01.64 Elbow.seek CC @15:13:04.59 Elbow.reconfigure LooseElbow @15:13:04.72 Forearm.reconfigure TightForearm @15:13:04.80 Forearm.seek CC @15:13:06.08 CC.seek :closed @15:13:10.39 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.40 @15:13:10.51 Hand.open @15:13:11.32 Forearm.reconfigure LooseForearm @15:13:11.40 Forearm.seek :retract @15:13:12.40 Flushing the Collection Valve::Manifold @15:13:12.56 CSV.dial CSR @15:13:13.13 Pulling 5ml of CSR.flush @15:13:13.15 CSR.select :flush @15:13:13.32 CS.seek 5ml @15:13:20.06 delay 3 seconds @15:13:23.08 ATV.dial :air3 @15:13:23.65 delay 3 seconds @15:13:26.73 AS.seek 0.794ml @15:13:27.24
delay 5 seconds @15:13:32.10 delay 3 seconds @15:13:32.25
CSR.select :air @15:13:32.42 CS.reconfigure CSfast @15:13:32.51 CS.seek 7.5ml @15:13:35.12 ATV.dial :bleach @15:13:35.77 AS.seek 0.779ml @15:13:39.06 delay 3 seconds @15:13:39.88
CSV.dial :waste @15:13:41.92 CS.reconfigure CSconfig @15:13:42.00 CS.seek 0ml @15:13:42.08 ATV.dial :AIV @15:13:43.41 AS.reconfigure ASconfig @15:13:43.49 AS.seek 0.015ml @15:14:02.13
Flushing lower manifold's waste1 port @15:14:02.30 CSV.dial :flush @15:14:02.83 CS.reconfigure CSfast @15:14:02.92 CS.seek 5ml @15:14:16.73 delay 5 seconds @15:14:21.75 CSV.dial :air @15:14:22.38 CS.seek 7ml @15:14:27.71 ATV.dial :waste @15:14:28.54 CSV.dial CTV @15:14:28.58
CTV.dial CSV @15:14:29.25 AS.seek :empty @15:14:30.21
CBV.dial :waste1 @15:14:30.49 ATV.dial :air3 @15:14:31.20
CS.seek 0ml @15:14:31.31 AS.reconfigure ASslow1 @15:14:31.38 AS.seek 0.009ml @15:14:33.74 delay 2 seconds @15:14:35.76 ATV.dial :bleach @15:14:36.25 AS.seek 0.579ml @15:14:50.07
Flushing lower manifold's waste2b port @15:14:50.24 CSV.dial :flush @15:14:51.13 CS.seek 5ml @15:15:04.92 delay 5 seconds @15:15:09.94 CSV.dial :air @15:15:10.69 CS.seek 7ml @15:15:16.76 CSV.dial CTV @15:15:16.80
CTV.dial CSV @15:15:17.38 CBV.dial :waste2b @15:15:18.29 CS.seek 0ml @15:15:37.15 Flushing lower manifold's waste1 port @15:15:37.16 CSV.dial CSR @15:15:37.79 Pulling 4ml of CSR.mfbkill @15:15:37.81 CSR.select :mfbkill @15:15:37.98 CS.reconfigure CSconfig @15:15:38.07 CS.seek 4ml @15:15:49.43 delay 5 seconds @15:15:54.44 CSR.select :air @15:15:54.60 CS.reconfigure CSfast @15:15:54.68 CS.seek 10ml @15:16:11.02 CSV.dial CTV @15:16:11.05
CTV.dial CSV @15:16:11.62 CBV.dial :waste1 @15:16:12.54 CS.seek 0ml @15:16:31.15 delay 3 seconds @15:16:34.17 ATV.dial :air3 @15:16:34.75 delay 3 seconds @15:16:37.82 AS.seek 0.604ml @15:16:39.11
Flushing lower manifold's waste2b port @15:16:39.12 CSV.dial CSR @15:16:39.74 Pulling 4ml of CSR.mfbkill @15:16:39.75 CSR.select :mfbkill @15:16:39.92 CS.reconfigure CSconfig @15:16:40.01 CS.seek 4ml @15:16:43.16 delay 3 seconds @15:16:46.18 ATV.dial :bleach @15:16:46.81 AS.seek 0.589ml @15:16:50.10 delay 3 seconds @15:16:51.32
delay 5 seconds @15:16:53.12 ATV.dial :ASV @15:16:54.20 ASV.dial :wasteR @15:16:54.83 AS.seek 0ml @15:16:56.34
CSR.select :air @15:16:56.50 CS.reconfigure CSfast @15:16:56.58 CS.seek 10ml @15:17:12.92 CSV.dial CTV @15:17:12.96
CTV.dial CSV @15:17:13.54 CBV.dial :waste2b @15:17:14.44 CS.seek 0ml @15:17:41.42 Killing upper manifold @15:17:41.43 CSV.dial CSR @15:17:42.07 Pulling 4ml of CSR.mfbkill @15:17:42.08 CSR.select :mfbkill @15:17:42.27 CS.reconfigure CSconfig @15:17:42.34 CS.seek 4ml @15:17:53.52 delay 5 seconds @15:17:58.53 CSR.select :air @15:17:58.70 CS.reconfigure CSfast @15:17:58.78 CS.seek 10ml @15:18:15.14 CSV.dial CBV @15:18:15.22
CBV.dial CSV @15:18:16.40 CTV.dial :air @15:18:17.99 CS.seek 0ml @15:18:44.48 delay 7 seconds @15:18:51.51 CS.seek 3ml @15:18:53.59 delay 1:00 @15:19:00.26
CSV.dial :waste @15:19:00.93 CS.seek 0ml @15:19:09.58 Flushing upper manifold @15:19:09.74 CSV.dial :flush @15:19:10.29 CS.seek 5ml @15:19:24.04 delay 5 seconds @15:19:29.06 CSV.dial :air @15:19:29.68 CS.seek 7ml @15:19:36.01 CSV.dial CBV @15:19:36.05
CBV.dial CSV @15:19:37.28 CTV.dial :air @15:19:37.31 CS.seek 0ml @15:19:53.61 AFV.select :carrierSolution @15:19:53.76 delay 2 seconds @15:19:55.78 AS.reconfigure ASconfig @15:19:55.86 AS.seek 1ml @15:19:56.08
delay 7 seconds @15:20:03.11 CS.seek 3ml @15:20:11.86 CSV.dial :waste @15:20:12.43 CS.seek 0ml @15:20:21.34 CSV.dial :flush @15:20:21.88 CS.seek 3ml @15:20:30.61 delay 5 seconds @15:20:35.63 CSV.dial :air @15:20:36.24 CS.seek 5ml @15:20:42.30 CSV.dial :air @15:20:42.36 CS.seek 0ml @15:20:53.64 delay 1 second @15:20:54.66 AFV.select :holdingCoil @15:20:54.80 delay 1 second @15:20:55.81 ATV.dial ASV @15:20:55.83 ASV.dial :wasteR @15:20:55.88 AS.reconfigure ASslow1 @15:20:55.98 AS.seek 0ml @15:20:56.07
delay 7 seconds @15:21:03.09 CS.seek 3ml @15:21:11.73 CSV.dial :waste @15:21:12.65 CS.seek 0ml @15:21:21.20 Flushing lower manifold's waste1 port @15:21:21.36 CSV.dial :flush @15:21:21.91 CS.seek 5ml @15:21:35.55 delay 5 seconds @15:21:40.57 CSV.dial :air @15:21:41.16 CS.seek 7ml @15:21:47.30 CSV.dial CTV @15:21:47.34
CTV.dial CSV @15:21:48.98 CBV.dial :waste1 @15:21:49.54 CS.seek 0ml @15:22:08.20 Flushing lower manifold's waste2b port @15:22:08.37 CSV.dial :flush @15:22:09.26 CS.seek 5ml @15:22:23.08 delay 5 seconds @15:22:28.10 CSV.dial :air @15:22:28.74 CS.seek 7ml @15:22:34.89 CSV.dial CTV @15:22:34.93
CTV.dial CSV @15:22:35.47 CBV.dial :waste2b @15:22:36.38 CS.seek 0ml @15:22:55.52 Garaging FlushPuck in Collection Clamp @15:22:55.64 Elbow.reconfigure TightElbow @15:22:55.72 Elbow.seek CC @15:22:55.99 Elbow.reconfigure LooseElbow @15:22:56.13 Forearm.seek CC @15:22:57.15 Hand.close @15:22:57.91 CC.seek :open @15:23:02.32 Forearm.seek :retract @15:23:03.33 Elbow.reconfigure TightElbow @15:23:03.41 Elbow.seek :garage @15:23:06.65 Elbow.reconfigure LooseElbow @15:23:06.78 Forearm.reconfigure TightForearm @15:23:06.86 Forearm.seek :garage @15:23:08.04 Hand.open @15:23:08.84 Forearm.reconfigure LooseForearm @15:23:08.92 Forearm.seek :retract @15:23:09.99 Starting initialPurge @15:23:10.00 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:23:10.12 Hand.open @15:23:10.46 Elbow.reconfigure TightElbow @15:23:10.55 Elbow.seek FlushPuck::Garage @15:23:10.71 Elbow.reconfigure LooseElbow @15:23:10.85 Forearm.seek :garage @15:23:11.88 Hand.close @15:23:12.77 Forearm.seek :retract @15:23:13.78 Elbow.reconfigure TightElbow @15:23:13.86 Elbow.seek CC @15:23:16.79 Elbow.reconfigure LooseElbow @15:23:16.92 Forearm.reconfigure TightForearm @15:23:16.99 Forearm.seek CC @15:23:18.25 CC.seek :closed @15:23:22.56 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.35 @15:23:22.68 Hand.open @15:23:23.46 Forearm.reconfigure LooseForearm @15:23:23.54 Forearm.seek :retract @15:23:24.59 Flushing the Collection Valve::Manifold @15:23:24.75 CSV.dial CSR @15:23:25.34 Pulling 5ml of CSR.flush @15:23:25.36 CSR.select :flush @15:23:25.53 CS.reconfigure CSconfig @15:23:25.60 CS.seek 5ml @15:23:39.46 delay 5 seconds @15:23:44.47 CSR.select :air @15:23:44.66 CS.reconfigure CSfast @15:23:44.74 CS.seek 7.501ml @15:23:52.14 CSV.dial :waste @15:23:53.68 CS.reconfigure CSconfig @15:23:53.77 CS.seek 0ml @15:24:14.02 Purging external Intake @15:24:14.18 CSV.dial :flush @15:24:14.71 CS.reconfigure CSfast @15:24:14.79 CS.seek 10ml @15:24:17.51 delay 20 seconds @15:24:37.54 ATV.dial :air3 @15:24:39.10 AS.seek 0.59ml @15:24:41.24
delay 5 seconds @15:24:46.76 CSV.dial CBV @15:24:46.79
CBV.dial CSV @15:24:47.74 CTV.dial :intake @15:24:48.64 Intake.select :open @15:24:48.79 CS.seek 0ml @15:25:16.42 Intake.select :close @15:25:16.57 CTV.dial :air @15:25:17.47 Flushing upper manifold @15:25:17.62 CSV.dial :flush @15:25:18.50 CS.seek 5ml @15:25:32.21 delay 5 seconds @15:25:37.23 CSV.dial :air @15:25:37.82 CS.seek 7ml @15:25:43.96 CSV.dial CBV @15:25:43.99
CBV.dial CSV @15:25:45.24 CTV.dial :air @15:25:45.27 CS.seek 0ml @15:26:04.21 delay 7 seconds @15:26:11.24 CS.seek 3ml @15:26:20.02 CSV.dial :waste @15:26:20.59 CS.seek 0ml @15:26:29.19 CSV.dial :flush @15:26:29.73 CS.seek 3ml @15:26:38.06 delay 3 seconds @15:26:38.36
delay 5 seconds @15:26:41.08 ATV.dial :ASV @15:26:42.66 ASV.dial :wasteR @15:26:42.77 AS.seek 0.09ml @15:26:43.38
CSV.dial :air @15:26:44.10 CS.seek 5ml @15:26:50.14 CSV.dial :air @15:26:50.20 CS.seek 0ml @15:27:04.05 delay 7 seconds @15:27:11.09 CS.seek 3ml @15:27:19.68 CSV.dial :waste @15:27:20.56 CS.seek 0ml @15:27:29.36 Garaging FlushPuck in Collection Clamp @15:27:29.48 Elbow.reconfigure TightElbow @15:27:29.55 Elbow.seek CC @15:27:29.82 Elbow.reconfigure LooseElbow @15:27:29.95 Forearm.seek CC @15:27:30.93 Hand.close @15:27:31.66 CC.seek :open @15:27:36.05 Forearm.seek :retract @15:27:37.04 Elbow.reconfigure TightElbow @15:27:37.12 Elbow.seek :garage @15:27:40.26 Elbow.reconfigure LooseElbow @15:27:40.39 Forearm.reconfigure TightForearm @15:27:40.46 Forearm.seek :garage @15:27:41.62 Hand.open @15:27:42.46 Forearm.reconfigure LooseForearm @15:27:42.54 Forearm.seek :retract @15:27:43.61 Completed [coreNEG] begun 13:54:40 @15:27:43.63 Extending 18tun until completion of MFBPCR @15:28:23.62 delay 30 seconds @15:28:53.65 ATV.dial :extSrc @15:28:54.44 delay 3 seconds @15:28:57.45 ATV.dial :ElutionWater @15:28:58.59 AS.seek 0.12ml @15:29:04.96 ATV.dial :air3 @15:29:05.59 AS.seek 0.13ml @15:29:07.90 delay 2 seconds @15:29:09.92 ATV.dial :bleach @15:29:10.40 AS.seek 0.23ml @15:29:30.79 delay 3 seconds @15:29:33.81 ATV.dial :air3 @15:29:34.39 delay 3 seconds @15:29:37.47 AS.seek 0.255ml @15:29:42.81 delay 3 seconds @15:29:45.83 ATV.dial :bleach @15:29:46.46 AS.seek 0.24ml @15:29:49.78 delay 3 seconds @15:29:52.80 ATV.dial :ASV @15:29:53.87 ASV.dial :SPEdelivery @15:29:54.75 AFV.select :carrierSolution @15:29:54.90 delay 2 seconds @15:29:56.92 AS.reconfigure ASconfig @15:29:57.00 AS.seek 0.5ml @15:30:12.26 delay 1 second @15:30:12.59 Session Ended @15:30:13.28 AFV.select :holdingCoil @15:30:13.45 AS.reconfigure ASslow1 @15:30:13.52 AS.seek 0.17ml @15:30:31.74 Session Ended @15:31:20.19 SPE.hold 2:46:40,95C @15:31:20.24 delay 3:00 @15:34:20.26 AFV.select :carrierSolution @15:34:20.38 delay 2 seconds @15:34:22.40 AS.reconfigure ASconfig @15:34:22.45 AS.seek 1ml @15:35:10.45 delay 1 second @15:35:11.47 AFV.select :holdingCoil @15:35:11.58 delay 1 second @15:35:12.59 ASV.dial :wasteD @15:35:13.69 AS.seek 0ml @15:35:41.52 Session Begun @15:35:45.43 -> showlog 100 @15:36:11.48 Priming Eluate @15:36:11.49 AFV.select :carrierSolution @15:36:11.65 delay 2 seconds @15:36:13.68 AS.seek 0.5ml @15:36:42.75 delay 1 second @15:36:43.77 AFV.select :holdingCoil @15:36:43.88 ATV.dial ASV @15:36:43.89 ASV.dial :SPErecovery @15:36:44.53 Swish #1 of 2 @15:36:44.63 AS.reconfigure ASslow0 @15:36:44.70 AS.seek 0.6ml @15:36:55.09 AS.seek 0.5ml @15:37:05.45 Swish #2 of 2 @15:37:05.55 AS.seek 0.6ml @15:37:16.04 AS.seek 0.5ml @15:37:26.43 delay 3 seconds @15:37:29.51 AS.reconfigure ASslow1 @15:37:29.58 AS.seek 0.684ml @15:38:06.87 delay 3 seconds @15:38:09.90 Eluate primed ATV.dial :waste2 @15:38:10.57 AS.reconfigure ASnorm @15:38:10.64 AS.seek 0ml @15:38:45.45 SPE.coast @15:38:45.54 ASV.dial :SPErecovery @15:38:45.57 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118aug1715h.pcr @15:38:45.59 Building PCR Slug from ASV with MM2 mix, PP4 primer @15:38:45.63 Priming with carrier @15:38:45.66 ATV.dial ARV @15:38:46.67 ARV.dial :carrier @15:38:47.04 AS.seek 0.5ml @15:39:12.58 AFV.select :carrierSolution @15:39:12.72 delay 2 seconds @15:39:14.74 AS.reconfigure ASconfig @15:39:14.81 AS.seek 1ml @15:39:43.84 delay 1 second @15:39:44.86 AFV.select :holdingCoil @15:39:44.97 delay 1 second @15:39:45.98 ATV.dial :waste @15:39:47.51 AS.reconfigure ASnorm @15:39:47.58 AS.seek :empty @15:40:38.36 AFV.select :carrierSolution @15:40:38.50 delay 2 seconds @15:40:40.52 AS.reconfigure ASconfig @15:40:40.59 AS.seek 0.5ml @15:41:09.65 delay 1 second @15:41:10.67 AFV.select :holdingCoil @15:41:10.79 Building Slug @15:41:10.80 ATV.dial :ARV @15:41:12.47 delay 2 seconds @15:41:14.49 ARV.dial :air1 @15:41:14.91 delay 2 seconds @15:41:16.98 AS.reconfigure ASslow2 @15:41:17.06 AS.seek 0.506ml @15:41:23.47 delay 2 seconds @15:41:25.49 ARV.dial :MM2 @15:41:27.01 delay 2 seconds @15:41:29.04 Adding MM2 @15:41:29.13 AS.seek 0.524ml @15:41:47.65 delay 25 seconds @15:42:12.67 ARV.dial :PP4 @15:42:13.09 Adding PP4 @15:42:13.10 delay 2 seconds @15:42:15.20 AS.seek 0.53ml @15:42:21.62 delay 10 seconds @15:42:31.65 ARV.dial :air3 @15:42:32.06 adding ARV air @15:42:32.07 delay 2 seconds @15:42:34.17 AS.seek 0.536ml @15:42:40.37 delay 5 seconds @15:42:45.39 ARV.dial :carrier @15:42:46.72 delay 7 seconds @15:42:53.81 AS.seek 0.594ml @15:43:52.30 delay 5 seconds @15:43:57.33 ATV.dial :ASV @15:43:57.83 delay 2 seconds @15:44:00.39 AS.seek 0.6ml @15:44:06.80 delay 4 seconds @15:44:10.82 ATV.dial ARV @15:44:11.37 delay 2 seconds @15:44:13.39 ARV.dial :carrier @15:44:13.41 delay 2 seconds @15:44:15.51 AS.seek 0.65ml @15:45:06.26 delay 4 seconds @15:45:10.28 ATV.dial PCR @15:45:10.91 ASV.dial :waste1 @15:45:11.73 ARV.dial :air1 @15:45:12.15 Positioning Slug by 0.324ml @15:45:12.25 AS.seek 0.326ml @15:50:38.14 delay 5 seconds @15:50:43.16 ATV.dial ARV @15:50:43.95 ASV.dial :PCRlock @15:50:44.34 delay 5 seconds @15:50:49.37 Running Tt1 Profile60C300 thermocycling @15:50:49.39 PCR.program Profile60C300 @15:50:49.55 Profile60C300 should complete in < 1:45:21 @15:50:49.56 PCR.run 2:16:41.25 @16:16:55.39 -> exit @16:16:55.40 Session Ended @17:11:45.86 PCR.off @17:11:47.87 PCR for Tt1 completed -- closed /var/log/esp/Tt118aug1715h.pcr @17:11:47.95 Cleaning PCR @17:11:47.96 AFV.select :carrierSolution @17:11:48.09 delay 2 seconds @17:11:50.11 AS.reconfigure ASconfig @17:11:50.17 AS.seek 0.5ml @17:12:00.49 delay 1 second @17:12:01.51 AFV.select :holdingCoil @17:12:01.62 ASV.dial :waste1 @17:12:02.00 ATV.dial :air3 @17:12:03.30 AS.reconfigure ASslow1 @17:12:03.37 AS.seek 0.51ml @17:12:05.64 delay 2 seconds @17:12:07.66 ATV.dial :bleach @17:12:08.28 AS.seek 0.81ml @17:13:08.89 delay 3 seconds @17:13:11.91 ATV.dial :air3 @17:13:12.47 delay 3 seconds @17:13:15.55 AS.seek 0.835ml @17:13:20.77 delay 3 seconds @17:13:23.79 ATV.dial :bleach @17:13:24.42 AS.seek 0.82ml @17:13:27.72 delay 3 seconds @17:13:30.74 ARV.dial :waste @17:13:31.36 delay 3 seconds @17:13:34.38 ATV.dial :ARV @17:13:35.28 AS.reconfigure ASconfig @17:13:35.34 AS.seek 0.47ml @17:13:55.74 AFV.select :carrierSolution @17:13:55.86 delay 2 seconds @17:13:57.88 AS.seek 1ml @17:14:28.67 delay 1 second @17:14:29.69 AFV.select :holdingCoil @17:14:29.80 delay 1 second @17:14:30.82 AS.reconfigure ASfast @17:14:30.88 AS.seek 0ml @17:14:58.31 AFV.select :carrierSolution @17:14:58.43 delay 2 seconds @17:15:00.45 AS.reconfigure ASconfig @17:15:00.50 AS.seek 1ml @17:15:58.28 delay 1 second @17:15:59.30 AFV.select :holdingCoil @17:15:59.41 delay 1 second @17:16:00.43 AS.reconfigure ASfast @17:16:00.49 AS.seek 0ml @17:16:27.86 AFV.select :carrierSolution @17:16:27.99 delay 2 seconds @17:16:30.01 AS.reconfigure ASconfig @17:16:30.06 AS.seek 1ml @17:17:27.80 delay 1 second @17:17:28.82 AFV.select :holdingCoil @17:17:28.93 delay 1 second @17:17:29.95 AS.reconfigure ASfast @17:17:30.01 AS.seek 0ml @17:17:57.36 ARV.dial between carrier and air1 @17:17:57.86 ATV.dial :air3 @17:17:59.16 AS.reconfigure ASslow1 @17:17:59.22 AS.seek 0.01ml @17:18:01.58 delay 2 seconds @17:18:03.60 ATV.dial :bleach @17:18:04.22 AS.seek 0.81ml @17:20:45.38 delay 3 seconds @17:20:48.40 ATV.dial :air3 @17:20:48.96 delay 3 seconds @17:20:52.04 AS.seek 0.835ml @17:20:57.34 delay 3 seconds @17:21:00.36 ATV.dial :bleach @17:21:00.99 AS.seek 0.82ml @17:21:04.30 delay 3 seconds @17:21:07.32 ATV.dial PCR @17:21:07.75 AS.reconfigure ASconfig @17:21:07.80 AS.seek :empty @17:21:55.27 PCR.program :bleachProfile @17:21:55.33 PCR program should complete in < 2:45 @17:21:55.34 PCR.run 10:00 @17:24:54.60 PCR.off @17:24:54.63 AFV.select :carrierSolution @17:24:54.75 delay 2 seconds @17:24:56.77 AS.seek 1ml @17:25:54.57 delay 1 second @17:25:55.59 AFV.select :holdingCoil @17:25:55.70 delay 1 second @17:25:56.72 AS.reconfigure ASfast @17:25:56.78 AS.seek 0ml @17:26:24.21 delay 3 seconds @17:26:27.23 AFV.select :carrierSolution @17:26:27.34 delay 2 seconds @17:26:29.36 AS.reconfigure ASconfig @17:26:29.42 AS.seek 1ml @17:27:27.18 delay 1 second @17:27:28.20 AFV.select :holdingCoil @17:27:28.31 delay 1 second @17:27:29.33 AS.reconfigure ASfast @17:27:29.39 AS.seek 0ml @17:27:56.74 delay 3 seconds @17:27:59.76 AFV.select :carrierSolution @17:27:59.87 delay 2 seconds @17:28:01.89 AS.reconfigure ASconfig @17:28:01.95 AS.seek 1ml @17:28:59.75 delay 1 second @17:29:00.77 AFV.select :holdingCoil @17:29:00.88 delay 1 second @17:29:01.90 AS.reconfigure ASfast @17:29:01.96 AS.seek 0ml @17:29:29.32 delay 3 seconds @17:29:32.34 AFV.select :carrierSolution @17:29:32.45 delay 2 seconds @17:29:34.47 AS.reconfigure ASconfig @17:29:34.53 AS.seek 1ml @17:30:32.33 delay 1 second @17:30:33.35 AFV.select :holdingCoil @17:30:33.46 delay 1 second @17:30:34.48 AS.reconfigure ASfast @17:30:34.54 AS.seek 0ml @17:31:01.91 delay 3 seconds @17:31:04.93 AFV.select :carrierSolution @17:31:05.04 delay 2 seconds @17:31:07.06 AS.reconfigure ASconfig @17:31:07.12 AS.seek 1ml @17:32:04.88 delay 1 second @17:32:05.90 AFV.select :holdingCoil @17:32:06.01 delay 1 second @17:32:07.03 AS.reconfigure ASnorm @17:32:07.09 AS.seek 0ml @17:32:57.81 Setting up PCR for IPc -- writing /var/log/esp/IPc18aug1717h.pcr @17:32:57.83 Building PCR Slug from ASV with MM1 mix, PP1 primer @17:32:57.85 Priming with carrier @17:32:57.88 ATV.dial ARV @17:32:58.49 ARV.dial :carrier @17:32:58.86 AS.seek 0.5ml @17:33:24.41 AFV.select :carrierSolution @17:33:24.54 delay 2 seconds @17:33:26.56 AS.reconfigure ASconfig @17:33:26.63 AS.seek 1ml @17:33:55.66 delay 1 second @17:33:56.68 AFV.select :holdingCoil @17:33:56.79 delay 1 second @17:33:57.80 ATV.dial :waste @17:33:59.56 AS.reconfigure ASnorm @17:33:59.62 AS.seek :empty @17:34:50.35 AFV.select :carrierSolution @17:34:50.47 delay 2 seconds @17:34:52.49 AS.reconfigure ASconfig @17:34:52.55 AS.seek 0.5ml @17:35:21.68 delay 1 second @17:35:22.70 AFV.select :holdingCoil @17:35:22.81 Building Slug @17:35:22.82 ATV.dial :ARV @17:35:24.46 delay 2 seconds @17:35:26.48 ARV.dial :air1 @17:35:26.89 delay 2 seconds @17:35:28.97 AS.reconfigure ASslow2 @17:35:29.03 AS.seek 0.506ml @17:35:35.26 delay 2 seconds @17:35:37.28 ARV.dial :MM1 @17:35:37.88 delay 2 seconds @17:35:39.91 Adding MM1 @17:35:39.99 AS.seek 0.524ml @17:35:58.26 delay 25 seconds @17:36:23.28 ARV.dial :PP1 @17:36:23.69 Adding PP1 @17:36:23.70 delay 2 seconds @17:36:25.78 AS.seek 0.53ml @17:36:32.13 delay 10 seconds @17:36:42.16 ARV.dial :air1 @17:36:42.55 adding ARV air @17:36:42.56 delay 2 seconds @17:36:44.64 AS.seek 0.536ml @17:36:51.13 delay 5 seconds @17:36:56.15 ARV.dial :carrier @17:36:56.57 delay 7 seconds @17:37:03.66 AS.seek 0.594ml @17:38:02.35 delay 5 seconds @17:38:07.37 ATV.dial :ASV @17:38:07.79 delay 2 seconds @17:38:09.87 AS.seek 0.6ml @17:38:16.60 delay 4 seconds @17:38:20.62 ATV.dial ARV @17:38:21.16 delay 2 seconds @17:38:23.18 ARV.dial :carrier @17:38:23.20 delay 2 seconds @17:38:25.28 AS.seek 0.65ml @17:39:15.80 delay 4 seconds @17:39:19.82 ATV.dial PCR @17:39:20.44 ASV.dial :waste1 @17:39:20.46 ARV.dial :air1 @17:39:20.89 Positioning Slug by 0.324ml @17:39:20.98 AS.seek 0.326ml @17:44:47.13 delay 5 seconds @17:44:52.15 ATV.dial ARV @17:44:53.02 ASV.dial :PCRlock @17:44:53.41 delay 5 seconds @17:44:58.44 Running IPc ProfileIPc thermocycling @17:44:58.45 PCR.program ProfileIPc @17:44:58.61 ProfileIPc should complete in < 1:50:49 @17:44:58.62 PCR.run 2:23:31.25 @19:08:00.04 PCR.off @19:08:02.58 PCR for IPc completed -- closed /var/log/esp/IPc18aug1717h.pcr @19:08:02.66 Cleaning PCR @19:08:02.67 AFV.select :carrierSolution @19:08:02.79 delay 2 seconds @19:08:04.81 AS.reconfigure ASconfig @19:08:04.87 AS.seek 0.5ml @19:08:15.21 delay 1 second @19:08:16.23 AFV.select :holdingCoil @19:08:16.34 ASV.dial :waste1 @19:08:16.71 ATV.dial :air3 @19:08:17.99 AS.reconfigure ASslow1 @19:08:18.05 AS.seek 0.51ml @19:08:20.35 delay 2 seconds @19:08:22.37 ATV.dial :bleach @19:08:22.99 AS.seek 0.81ml @19:09:23.59 delay 3 seconds @19:09:26.61 ATV.dial :air3 @19:09:27.18 delay 3 seconds @19:09:30.26 AS.seek 0.835ml @19:09:35.57 delay 3 seconds @19:09:38.59 ATV.dial :bleach @19:09:39.21 AS.seek 0.82ml @19:09:42.46 delay 3 seconds @19:09:45.48 ARV.dial :waste @19:09:46.10 delay 3 seconds @19:09:49.12 ATV.dial :ARV @19:09:50.02 AS.reconfigure ASconfig @19:09:50.08 AS.seek 0.47ml @19:10:10.46 AFV.select :carrierSolution @19:10:10.58 delay 2 seconds @19:10:12.60 AS.seek 1ml @19:10:43.38 delay 1 second @19:10:44.40 AFV.select :holdingCoil @19:10:44.51 delay 1 second @19:10:45.53 AS.reconfigure ASfast @19:10:45.59 AS.seek 0ml @19:11:13.09 AFV.select :carrierSolution @19:11:13.21 delay 2 seconds @19:11:15.23 AS.reconfigure ASconfig @19:11:15.29 AS.seek 1ml @19:12:13.09 delay 1 second @19:12:14.11 AFV.select :holdingCoil @19:12:14.22 delay 1 second @19:12:15.24 AS.reconfigure ASfast @19:12:15.30 AS.seek 0ml @19:12:42.95 AFV.select :carrierSolution @19:12:43.07 delay 2 seconds @19:12:45.09 AS.reconfigure ASconfig @19:12:45.14 AS.seek 1ml @19:13:42.88 delay 1 second @19:13:43.90 AFV.select :holdingCoil @19:13:44.01 delay 1 second @19:13:45.03 AS.reconfigure ASfast @19:13:45.09 AS.seek 0ml @19:14:12.70 ARV.dial between carrier and air1 @19:14:13.21 ATV.dial :air3 @19:14:14.51 AS.reconfigure ASslow1 @19:14:14.57 AS.seek 0.01ml @19:14:16.85 delay 2 seconds @19:14:18.87 ATV.dial :bleach @19:14:19.49 AS.seek 0.81ml @19:17:00.62 delay 3 seconds @19:17:03.64 ATV.dial :air3 @19:17:04.67 delay 3 seconds @19:17:07.75 AS.seek 0.835ml @19:17:13.07 delay 3 seconds @19:17:16.09 ATV.dial :bleach @19:17:16.71 AS.seek 0.82ml @19:17:19.96 delay 3 seconds @19:17:22.98 ATV.dial PCR @19:17:23.41 AS.reconfigure ASconfig @19:17:23.46 AS.seek :empty @19:18:10.93 PCR.program :bleachProfile @19:18:10.99 PCR program should complete in < 2:45 @19:18:11.00 PCR.run 10:00 @19:21:12.82 PCR.off @19:21:12.86 AFV.select :carrierSolution @19:21:12.98 delay 2 seconds @19:21:15.00 AS.seek 1ml @19:22:12.84 delay 1 second @19:22:13.86 AFV.select :holdingCoil @19:22:13.97 delay 1 second @19:22:14.99 AS.reconfigure ASfast @19:22:15.05 AS.seek 0ml @19:22:42.50 delay 3 seconds @19:22:45.52 AFV.select :carrierSolution @19:22:45.63 delay 2 seconds @19:22:47.65 AS.reconfigure ASconfig @19:22:47.71 AS.seek 1ml @19:23:45.45 delay 1 second @19:23:46.47 AFV.select :holdingCoil @19:23:46.58 delay 1 second @19:23:47.60 AS.reconfigure ASfast @19:23:47.66 AS.seek 0ml @19:24:15.17 delay 3 seconds @19:24:18.19 AFV.select :carrierSolution @19:24:18.30 delay 2 seconds @19:24:20.32 AS.reconfigure ASconfig @19:24:20.38 AS.seek 1ml @19:25:18.14 delay 1 second @19:25:19.16 AFV.select :holdingCoil @19:25:19.27 delay 1 second @19:25:20.29 AS.reconfigure ASfast @19:25:20.35 AS.seek 0ml @19:25:47.82 delay 3 seconds @19:25:50.84 AFV.select :carrierSolution @19:25:50.95 delay 2 seconds @19:25:52.97 AS.reconfigure ASconfig @19:25:53.03 AS.seek 1ml @19:26:50.79 delay 1 second @19:26:51.81 AFV.select :holdingCoil @19:26:51.92 delay 1 second @19:26:52.94 AS.reconfigure ASfast @19:26:53.00 AS.seek 0ml @19:27:20.40 delay 3 seconds @19:27:23.42 AFV.select :carrierSolution @19:27:23.53 delay 2 seconds @19:27:25.55 AS.reconfigure ASconfig @19:27:25.61 AS.seek 1ml @19:28:23.36 delay 1 second @19:28:24.38 AFV.select :holdingCoil @19:28:24.49 delay 1 second @19:28:25.51 AS.reconfigure ASnorm @19:28:25.57 AS.seek 0ml @19:29:16.28 Setting up PCR for Rp -- writing /var/log/esp/Rp18aug1719h.pcr @19:29:16.30 Building PCR Slug from ASV with MM2 mix, PP3 primer @19:29:16.31 Priming with carrier @19:29:16.34 ATV.dial ARV @19:29:17.06 ARV.dial :carrier @19:29:17.42 AS.seek 0.5ml @19:29:42.98 AFV.select :carrierSolution @19:29:43.10 delay 2 seconds @19:29:45.12 AS.reconfigure ASconfig @19:29:45.19 AS.seek 1ml @19:30:14.22 delay 1 second @19:30:15.24 AFV.select :holdingCoil @19:30:15.35 delay 1 second @19:30:16.36 ATV.dial :waste @19:30:18.04 AS.reconfigure ASnorm @19:30:18.10 AS.seek :empty @19:31:08.86 AFV.select :carrierSolution @19:31:08.99 delay 2 seconds @19:31:11.01 AS.reconfigure ASconfig @19:31:11.08 AS.seek 0.5ml @19:31:40.19 delay 1 second @19:31:41.21 AFV.select :holdingCoil @19:31:41.32 Building Slug @19:31:41.33 ATV.dial :ARV @19:31:42.98 delay 2 seconds @19:31:45.00 ARV.dial :air1 @19:31:45.41 delay 2 seconds @19:31:47.48 AS.reconfigure ASslow2 @19:31:47.55 AS.seek 0.506ml @19:31:53.76 delay 2 seconds @19:31:55.78 ARV.dial :MM2 @19:31:57.28 delay 2 seconds @19:31:59.31 Adding MM2 @19:31:59.40 AS.seek 0.524ml @19:32:17.92 delay 25 seconds @19:32:42.94 ARV.dial :PP3 @19:32:43.35 Adding PP3 @19:32:43.85 delay 2 seconds @19:32:45.95 AS.seek 0.53ml @19:32:52.12 delay 10 seconds @19:33:02.15 ARV.dial :air2 @19:33:02.56 adding ARV air @19:33:02.57 delay 2 seconds @19:33:04.65 AS.seek 0.536ml @19:33:11.11 delay 5 seconds @19:33:16.13 ARV.dial :carrier @19:33:17.44 delay 7 seconds @19:33:24.53 AS.seek 0.594ml @19:34:23.05 delay 5 seconds @19:34:28.07 ATV.dial :ASV @19:34:28.48 delay 2 seconds @19:34:30.56 AS.seek 0.6ml @19:34:36.87 delay 4 seconds @19:34:40.89 ATV.dial ARV @19:34:41.44 delay 2 seconds @19:34:43.46 ARV.dial :carrier @19:34:43.48 delay 2 seconds @19:34:45.56 AS.seek 0.65ml @19:35:36.42 delay 4 seconds @19:35:40.44 ATV.dial PCR @19:35:41.06 ASV.dial :waste1 @19:35:41.08 ARV.dial :air1 @19:35:41.52 Positioning Slug by 0.324ml @19:35:41.61 AS.seek 0.326ml @19:41:07.70 delay 5 seconds @19:41:12.72 ATV.dial ARV @19:41:13.50 ASV.dial :PCRlock @19:41:13.89 delay 5 seconds @19:41:18.92 Running Rp Profile60C300 thermocycling @19:41:18.93 PCR.program Profile60C300 @19:41:19.08 Profile60C300 should complete in < 1:45:21 @19:41:19.09 PCR.run 2:16:41.25 @21:02:04.43 PCR.off @21:02:05.96 PCR for Rp completed -- closed /var/log/esp/Rp18aug1719h.pcr @21:02:06.04 Cleaning PCR @21:02:06.05 AFV.select :carrierSolution @21:02:06.17 delay 2 seconds @21:02:08.19 AS.reconfigure ASconfig @21:02:08.25 AS.seek 0.5ml @21:02:18.55 delay 1 second @21:02:19.57 AFV.select :holdingCoil @21:02:19.68 ASV.dial :waste1 @21:02:20.05 ATV.dial :air3 @21:02:21.34 AS.reconfigure ASslow1 @21:02:21.40 AS.seek 0.51ml @21:02:23.69 delay 2 seconds @21:02:25.71 ATV.dial :bleach @21:02:26.33 AS.seek 0.81ml @21:03:26.92 delay 3 seconds @21:03:29.94 ATV.dial :air3 @21:03:30.50 delay 3 seconds @21:03:33.58 AS.seek 0.835ml @21:03:38.80 delay 3 seconds @21:03:41.82 ATV.dial :bleach @21:03:42.45 AS.seek 0.82ml @21:03:45.74 delay 3 seconds @21:03:48.76 ARV.dial :waste @21:03:49.39 delay 3 seconds @21:03:52.41 ATV.dial :ARV @21:03:53.31 AS.reconfigure ASconfig @21:03:53.37 AS.seek 0.47ml @21:04:13.78 AFV.select :carrierSolution @21:04:13.90 delay 2 seconds @21:04:15.92 AS.seek 1ml @21:04:46.66 delay 1 second @21:04:47.68 AFV.select :holdingCoil @21:04:47.79 delay 1 second @21:04:48.81 AS.reconfigure ASfast @21:04:48.87 AS.seek 0ml @21:05:16.24 AFV.select :carrierSolution @21:05:16.36 delay 2 seconds @21:05:18.38 AS.reconfigure ASconfig @21:05:18.44 AS.seek 1ml @21:06:16.19 delay 1 second @21:06:17.21 AFV.select :holdingCoil @21:06:17.32 delay 1 second @21:06:18.34 AS.reconfigure ASfast @21:06:18.40 AS.seek 0ml @21:06:46.03 AFV.select :carrierSolution @21:06:46.15 delay 2 seconds @21:06:48.17 AS.reconfigure ASconfig @21:06:48.23 AS.seek 1ml @21:07:45.99 delay 1 second @21:07:47.01 AFV.select :holdingCoil @21:07:47.12 delay 1 second @21:07:48.14 AS.reconfigure ASfast @21:07:48.20 AS.seek 0ml @21:08:15.71 ARV.dial between carrier and air1 @21:08:16.24 ATV.dial :air3 @21:08:17.55 AS.reconfigure ASslow1 @21:08:17.61 AS.seek 0.01ml @21:08:19.97 delay 2 seconds @21:08:21.99 ATV.dial :bleach @21:08:22.61 AS.seek 0.81ml @21:11:03.77 delay 3 seconds @21:11:06.79 ATV.dial :air3 @21:11:07.35 delay 3 seconds @21:11:10.43 AS.seek 0.835ml @21:11:15.64 delay 3 seconds @21:11:18.66 ATV.dial :bleach @21:11:19.28 AS.seek 0.82ml @21:11:22.60 delay 3 seconds @21:11:25.62 ATV.dial PCR @21:11:26.04 AS.reconfigure ASconfig @21:11:26.09 AS.seek :empty @21:12:13.58 PCR.program :bleachProfile @21:12:13.64 PCR program should complete in < 2:45 @21:12:13.65 PCR.run 10:00 @21:15:12.94 PCR.off @21:15:12.98 AFV.select :carrierSolution @21:15:13.10 delay 2 seconds @21:15:15.12 AS.seek 1ml @21:16:12.96 delay 1 second @21:16:13.98 AFV.select :holdingCoil @21:16:14.09 delay 1 second @21:16:15.11 AS.reconfigure ASfast @21:16:15.17 AS.seek 0ml @21:16:42.50 delay 3 seconds @21:16:45.52 AFV.select :carrierSolution @21:16:45.63 delay 2 seconds @21:16:47.65 AS.reconfigure ASconfig @21:16:47.71 AS.seek 1ml @21:17:45.52 delay 1 second @21:17:46.54 AFV.select :holdingCoil @21:17:46.65 delay 1 second @21:17:47.67 AS.reconfigure ASfast @21:17:47.73 AS.seek 0ml @21:18:15.11 delay 3 seconds @21:18:18.13 AFV.select :carrierSolution @21:18:18.24 delay 2 seconds @21:18:20.26 AS.reconfigure ASconfig @21:18:20.33 AS.seek 1ml @21:19:18.10 delay 1 second @21:19:19.12 AFV.select :holdingCoil @21:19:19.23 delay 1 second @21:19:20.25 AS.reconfigure ASfast @21:19:20.31 AS.seek 0ml @21:19:47.65 delay 3 seconds @21:19:50.67 AFV.select :carrierSolution @21:19:50.78 delay 2 seconds @21:19:52.80 AS.reconfigure ASconfig @21:19:52.86 AS.seek 1ml @21:20:50.63 delay 1 second @21:20:51.65 AFV.select :holdingCoil @21:20:51.76 delay 1 second @21:20:52.78 AS.reconfigure ASfast @21:20:52.84 AS.seek 0ml @21:21:20.19 delay 3 seconds @21:21:23.21 AFV.select :carrierSolution @21:21:23.32 delay 2 seconds @21:21:25.34 AS.reconfigure ASconfig @21:21:25.40 AS.seek 1ml @21:22:23.18 delay 1 second @21:22:24.20 AFV.select :holdingCoil @21:22:24.31 delay 1 second @21:22:25.33 AS.reconfigure ASnorm @21:22:25.39 AS.seek 0ml @21:23:16.12 Setting up PCR for Ss -- writing /var/log/esp/Ss18aug1721h.pcr @21:23:16.14 Building PCR Slug from ASV with MM1 mix, PP2 primer @21:23:16.17 Priming with carrier @21:23:16.19 ATV.dial ARV @21:23:16.91 ARV.dial :carrier @21:23:17.27 AS.seek 0.5ml @21:23:42.85 AFV.select :carrierSolution @21:23:42.97 delay 2 seconds @21:23:44.99 AS.reconfigure ASconfig @21:23:45.06 AS.seek 1ml @21:24:14.10 delay 1 second @21:24:15.12 AFV.select :holdingCoil @21:24:15.23 delay 1 second @21:24:16.24 ATV.dial :waste @21:24:17.91 AS.reconfigure ASnorm @21:24:17.97 AS.seek :empty @21:25:08.72 AFV.select :carrierSolution @21:25:08.84 delay 2 seconds @21:25:10.86 AS.reconfigure ASconfig @21:25:10.92 AS.seek 0.5ml @21:25:40.02 delay 1 second @21:25:41.04 AFV.select :holdingCoil @21:25:41.15 Building Slug @21:25:41.16 ATV.dial :ARV @21:25:42.80 delay 2 seconds @21:25:44.82 ARV.dial :air1 @21:25:45.24 delay 2 seconds @21:25:47.30 AS.reconfigure ASslow2 @21:25:47.37 AS.seek 0.506ml @21:25:53.77 delay 2 seconds @21:25:55.79 ARV.dial :MM1 @21:25:56.40 delay 2 seconds @21:25:58.43 Adding MM1 @21:25:58.51 AS.seek 0.524ml @21:26:16.74 delay 25 seconds @21:26:41.76 ARV.dial :PP2 @21:26:42.16 Adding PP2 @21:26:42.18 delay 2 seconds @21:26:44.26 AS.seek 0.53ml @21:26:50.43 delay 10 seconds @21:27:00.46 ARV.dial :air2 @21:27:00.88 adding ARV air @21:27:00.89 delay 2 seconds @21:27:02.97 AS.seek 0.536ml @21:27:09.22 delay 5 seconds @21:27:14.24 ARV.dial :carrier @21:27:15.54 delay 7 seconds @21:27:22.63 AS.seek 0.594ml @21:28:21.07 delay 5 seconds @21:28:26.09 ATV.dial :ASV @21:28:26.51 delay 2 seconds @21:28:29.09 AS.seek 0.6ml @21:28:35.37 delay 4 seconds @21:28:39.39 ATV.dial ARV @21:28:39.93 delay 2 seconds @21:28:41.95 ARV.dial :carrier @21:28:41.97 delay 2 seconds @21:28:44.05 AS.seek 0.65ml @21:29:34.52 delay 4 seconds @21:29:38.54 ATV.dial PCR @21:29:39.16 ASV.dial :waste1 @21:29:39.18 ARV.dial :air1 @21:29:39.61 Positioning Slug by 0.324ml @21:29:39.70 AS.seek 0.326ml @21:35:05.82 delay 5 seconds @21:35:10.84 ATV.dial ARV @21:35:11.63 ASV.dial :PCRlock @21:35:12.02 delay 5 seconds @21:35:17.05 Running Ss Profile60C350 thermocycling @21:35:17.06 PCR.program Profile60C350 @21:35:17.22 Profile60C350 should complete in < 1:48:05 @21:35:17.23 PCR.run 2:20:06.25 @22:56:34.40 PCR.off @22:56:36.56 PCR for Ss completed -- closed /var/log/esp/Ss18aug1721h.pcr @22:56:36.63 Cleaning PCR @22:56:36.64 AFV.select :carrierSolution @22:56:36.77 delay 2 seconds @22:56:38.79 AS.reconfigure ASconfig @22:56:38.85 AS.seek 0.5ml @22:56:49.17 delay 1 second @22:56:50.19 AFV.select :holdingCoil @22:56:50.30 ASV.dial :waste1 @22:56:50.67 ATV.dial :air3 @22:56:51.97 AS.reconfigure ASslow1 @22:56:52.03 AS.seek 0.51ml @22:56:54.36 delay 2 seconds @22:56:56.38 ATV.dial :bleach @22:56:57.00 AS.seek 0.81ml @22:57:57.64 delay 3 seconds @22:58:00.66 ATV.dial :air3 @22:58:01.22 delay 3 seconds @22:58:04.30 AS.seek 0.835ml @22:58:09.58 delay 3 seconds @22:58:12.60 ATV.dial :bleach @22:58:13.22 AS.seek 0.82ml @22:58:16.55 delay 3 seconds @22:58:19.57 ARV.dial :waste @22:58:20.19 delay 3 seconds @22:58:23.21 ATV.dial :ARV @22:58:24.11 AS.reconfigure ASconfig @22:58:24.17 AS.seek 0.47ml @22:58:44.58 AFV.select :carrierSolution @22:58:44.70 delay 2 seconds @22:58:46.72 AS.seek 1ml @22:59:17.49 delay 1 second @22:59:18.51 AFV.select :holdingCoil @22:59:18.62 delay 1 second @22:59:19.64 AS.reconfigure ASfast @22:59:19.70 AS.seek 0ml @22:59:47.03 AFV.select :carrierSolution @22:59:47.15 delay 2 seconds @22:59:49.17 AS.reconfigure ASconfig @22:59:49.23 AS.seek 1ml @23:00:46.99 delay 1 second @23:00:48.01 AFV.select :holdingCoil @23:00:48.12 delay 1 second @23:00:49.14 AS.reconfigure ASfast @23:00:49.20 AS.seek 0ml @23:01:16.53 AFV.select :carrierSolution @23:01:16.65 delay 2 seconds @23:01:18.67 AS.reconfigure ASconfig @23:01:18.73 AS.seek 1ml @23:02:16.49 delay 1 second @23:02:17.51 AFV.select :holdingCoil @23:02:17.62 delay 1 second @23:02:18.64 AS.reconfigure ASfast @23:02:18.70 AS.seek 0ml @23:02:46.18 ARV.dial between carrier and air1 @23:02:46.71 ATV.dial :air3 @23:02:48.00 AS.reconfigure ASslow1 @23:02:48.06 AS.seek 0.01ml @23:02:50.39 delay 2 seconds @23:02:52.41 ATV.dial :bleach @23:02:53.03 AS.seek 0.81ml @23:05:34.22 delay 3 seconds @23:05:37.24 ATV.dial :air3 @23:05:37.80 delay 3 seconds @23:05:40.88 AS.seek 0.835ml @23:05:46.19 delay 3 seconds @23:05:49.21 ATV.dial :bleach @23:05:49.83 AS.seek 0.82ml @23:05:53.13 delay 3 seconds @23:05:56.15 ATV.dial PCR @23:05:56.56 AS.reconfigure ASconfig @23:05:56.62 AS.seek :empty @23:06:44.10 PCR.program :bleachProfile @23:06:44.16 PCR program should complete in < 2:45 @23:06:44.17 PCR.run 10:00 @23:09:42.96 PCR.off @23:09:43.00 AFV.select :carrierSolution @23:09:43.12 delay 2 seconds @23:09:45.14 AS.seek 1ml @23:10:43.44 delay 1 second @23:10:44.46 AFV.select :holdingCoil @23:10:44.57 delay 1 second @23:10:45.59 AS.reconfigure ASfast @23:10:45.65 AS.seek 0ml @23:11:12.99 delay 3 seconds @23:11:16.01 AFV.select :carrierSolution @23:11:16.12 delay 2 seconds @23:11:18.14 AS.reconfigure ASconfig @23:11:18.20 AS.seek 1ml @23:12:16.01 delay 1 second @23:12:17.03 AFV.select :holdingCoil @23:12:17.14 delay 1 second @23:12:18.16 AS.reconfigure ASfast @23:12:18.22 AS.seek 0ml @23:12:45.72 delay 3 seconds @23:12:48.74 AFV.select :carrierSolution @23:12:48.85 delay 2 seconds @23:12:50.87 AS.reconfigure ASconfig @23:12:50.94 AS.seek 1ml @23:13:48.74 delay 1 second @23:13:49.76 AFV.select :holdingCoil @23:13:49.87 delay 1 second @23:13:50.89 AS.reconfigure ASfast @23:13:50.95 AS.seek 0ml @23:14:18.30 delay 3 seconds @23:14:21.32 AFV.select :carrierSolution @23:14:21.43 delay 2 seconds @23:14:23.45 AS.reconfigure ASconfig @23:14:23.51 AS.seek 1ml @23:15:21.29 delay 1 second @23:15:22.31 AFV.select :holdingCoil @23:15:22.42 delay 1 second @23:15:23.44 AS.reconfigure ASfast @23:15:23.50 AS.seek 0ml @23:15:50.85 delay 3 seconds @23:15:53.87 AFV.select :carrierSolution @23:15:53.98 delay 2 seconds @23:15:56.00 AS.reconfigure ASconfig @23:15:56.07 AS.seek 1ml @23:16:53.83 delay 1 second @23:16:54.85 AFV.select :holdingCoil @23:16:54.96 delay 1 second @23:16:55.98 AS.reconfigure ASnorm @23:16:56.04 AS.seek 0ml @23:17:46.78 Setting up PCR for Bb -- writing /var/log/esp/Bb18aug1723h.pcr @23:17:46.80 Building PCR Slug from ASV with MM3 mix, PP5 primer @23:17:46.82 Priming with carrier @23:17:46.84 ATV.dial ARV @23:17:47.48 ARV.dial :carrier @23:17:47.84 AS.seek 0.5ml @23:18:13.40 AFV.select :carrierSolution @23:18:13.52 delay 2 seconds @23:18:15.54 AS.reconfigure ASconfig @23:18:15.61 AS.seek 1ml @23:18:44.65 delay 1 second @23:18:45.67 AFV.select :holdingCoil @23:18:45.78 delay 1 second @23:18:46.79 ATV.dial :waste @23:18:48.47 AS.reconfigure ASnorm @23:18:48.53 AS.seek :empty @23:19:39.27 AFV.select :carrierSolution @23:19:39.39 delay 2 seconds @23:19:41.41 AS.reconfigure ASconfig @23:19:41.47 AS.seek 0.5ml @23:20:10.58 delay 1 second @23:20:11.60 AFV.select :holdingCoil @23:20:11.71 Building Slug @23:20:11.72 ATV.dial :ARV @23:20:13.37 delay 2 seconds @23:20:15.39 ARV.dial :air1 @23:20:15.81 delay 2 seconds @23:20:17.88 AS.reconfigure ASslow2 @23:20:17.95 AS.seek 0.506ml @23:20:24.16 delay 2 seconds @23:20:26.18 ARV.dial :MM3 @23:20:27.26 delay 2 seconds @23:20:29.29 Adding MM3 @23:20:29.38 AS.seek 0.524ml @23:20:47.80 delay 25 seconds @23:21:12.82 ARV.dial :PP5 @23:21:13.23 Adding PP5 @23:21:13.25 delay 2 seconds @23:21:15.33 AS.seek 0.53ml @23:21:21.77 delay 10 seconds @23:21:31.80 ARV.dial :air3 @23:21:32.19 adding ARV air @23:21:32.21 delay 2 seconds @23:21:34.29 AS.seek 0.536ml @23:21:40.73 delay 5 seconds @23:21:45.75 ARV.dial :carrier @23:21:47.04 delay 7 seconds @23:21:54.13 AS.seek 0.594ml @23:22:52.68 delay 5 seconds @23:22:57.70 ATV.dial :ASV @23:22:58.12 delay 2 seconds @23:23:00.20 AS.seek 0.6ml @23:23:06.40 delay 4 seconds @23:23:10.42 ATV.dial ARV @23:23:10.98 delay 2 seconds @23:23:13.00 ARV.dial :carrier @23:23:13.02 delay 2 seconds @23:23:15.10 AS.seek 0.65ml @23:24:05.80 delay 4 seconds @23:24:09.83 ATV.dial PCR @23:24:10.45 ASV.dial :waste1 @23:24:10.47 ARV.dial :air1 @23:24:11.41 Positioning Slug by 0.324ml @23:24:11.50 AS.seek 0.326ml @23:29:37.37 delay 5 seconds @23:29:42.39 ATV.dial ARV @23:29:43.18 ASV.dial :PCRlock @23:29:43.57 delay 5 seconds @23:29:48.60 Running Bb Profile60C300 thermocycling @23:29:48.61 PCR.program Profile60C300 @23:29:48.76 Profile60C300 should complete in < 1:45:21 @23:29:48.77 PCR.run 2:16:41.25 @00:50:10.69CEST18-Aug-18 PCR.off @00:50:12.21 PCR for Bb completed -- closed /var/log/esp/Bb18aug1723h.pcr @00:50:12.29 Cleaning PCR @00:50:12.31 AFV.select :carrierSolution @00:50:12.44 delay 2 seconds @00:50:14.46 AS.reconfigure ASconfig @00:50:14.52 AS.seek 0.5ml @00:50:24.84 delay 1 second @00:50:25.86 AFV.select :holdingCoil @00:50:25.97 ASV.dial :waste1 @00:50:26.35 ATV.dial :air3 @00:50:27.65 AS.reconfigure ASslow1 @00:50:28.22 AS.seek 0.51ml @00:50:30.52 delay 2 seconds @00:50:32.54 ATV.dial :bleach @00:50:33.16 AS.seek 0.81ml @00:51:33.74 delay 3 seconds @00:51:36.76 ATV.dial :air3 @00:51:37.32 delay 3 seconds @00:51:40.40 AS.seek 0.835ml @00:51:45.73 delay 3 seconds @00:51:48.75 ATV.dial :bleach @00:51:49.37 AS.seek 0.82ml @00:51:52.70 delay 3 seconds @00:51:55.72 ARV.dial :waste @00:51:56.34 delay 3 seconds @00:51:59.36 ATV.dial :ARV @00:52:00.26 AS.reconfigure ASconfig @00:52:00.32 AS.seek 0.47ml @00:52:20.74 AFV.select :carrierSolution @00:52:20.86 delay 2 seconds @00:52:22.88 AS.seek 1ml @00:52:53.63 delay 1 second @00:52:54.65 AFV.select :holdingCoil @00:52:54.76 delay 1 second @00:52:55.78 AS.reconfigure ASfast @00:52:55.85 AS.seek 0ml @00:53:23.21 AFV.select :carrierSolution @00:53:23.33 delay 2 seconds @00:53:25.35 AS.reconfigure ASconfig @00:53:25.41 AS.seek 1ml @00:54:23.17 delay 1 second @00:54:24.19 AFV.select :holdingCoil @00:54:24.30 delay 1 second @00:54:25.32 AS.reconfigure ASfast @00:54:25.38 AS.seek 0ml @00:54:52.96 AFV.select :carrierSolution @00:54:53.08 delay 2 seconds @00:54:55.10 AS.reconfigure ASconfig @00:54:55.16 AS.seek 1ml @00:55:52.90 delay 1 second @00:55:53.92 AFV.select :holdingCoil @00:55:54.03 delay 1 second @00:55:55.05 AS.reconfigure ASfast @00:55:55.11 AS.seek 0ml @00:56:22.63 ARV.dial between carrier and air1 @00:56:23.13 ATV.dial :air3 @00:56:24.41 AS.reconfigure ASslow1 @00:56:24.47 AS.seek 0.01ml @00:56:26.78 delay 2 seconds @00:56:28.80 ATV.dial :bleach @00:56:29.41 AS.seek 0.81ml @00:59:10.57 delay 3 seconds @00:59:13.59 ATV.dial :air3 @00:59:14.15 delay 3 seconds @00:59:17.23 AS.seek 0.835ml @00:59:22.55 delay 3 seconds @00:59:25.57 ATV.dial :bleach @00:59:26.20 AS.seek 0.82ml @00:59:29.46 delay 3 seconds @00:59:32.48 ATV.dial PCR @00:59:32.91 AS.reconfigure ASconfig @00:59:32.96 AS.seek :empty @01:00:20.43 PCR.program :bleachProfile @01:00:20.49 PCR program should complete in < 2:45 @01:00:20.50 PCR.run 10:00 @01:03:19.30 PCR.off @01:03:19.34 AFV.select :carrierSolution @01:03:19.46 delay 2 seconds @01:03:21.48 AS.seek 1ml @01:04:19.28 delay 1 second @01:04:20.30 AFV.select :holdingCoil @01:04:20.41 delay 1 second @01:04:21.43 AS.reconfigure ASfast @01:04:21.49 AS.seek 0ml @01:04:49.04 delay 3 seconds @01:04:52.06 AFV.select :carrierSolution @01:04:52.17 delay 2 seconds @01:04:54.19 AS.reconfigure ASconfig @01:04:54.25 AS.seek 1ml @01:05:52.01 delay 1 second @01:05:53.03 AFV.select :holdingCoil @01:05:53.14 delay 1 second @01:05:54.16 AS.reconfigure ASfast @01:05:54.22 AS.seek 0ml @01:06:21.56 delay 3 seconds @01:06:24.58 AFV.select :carrierSolution @01:06:24.69 delay 2 seconds @01:06:26.71 AS.reconfigure ASconfig @01:06:26.77 AS.seek 1ml @01:07:24.54 delay 1 second @01:07:25.56 AFV.select :holdingCoil @01:07:25.67 delay 1 second @01:07:26.69 AS.reconfigure ASfast @01:07:26.75 AS.seek 0ml @01:07:54.11 delay 3 seconds @01:07:57.63 AFV.select :carrierSolution @01:07:57.74 delay 2 seconds @01:07:59.76 AS.reconfigure ASconfig @01:07:59.82 AS.seek 1ml @01:08:57.59 delay 1 second @01:08:58.61 AFV.select :holdingCoil @01:08:58.72 delay 1 second @01:08:59.74 AS.reconfigure ASfast @01:08:59.80 AS.seek 0ml @01:09:27.14 delay 3 seconds @01:09:30.16 AFV.select :carrierSolution @01:09:30.27 delay 2 seconds @01:09:32.29 AS.reconfigure ASconfig @01:09:32.35 AS.seek 1ml @01:10:30.15 delay 1 second @01:10:31.17 AFV.select :holdingCoil @01:10:31.28 delay 1 second @01:10:32.30 AS.reconfigure ASnorm @01:10:32.36 AS.seek 0ml @01:11:23.06 Setting up PCR for Tt2 -- writing /var/log/esp/Tt218aug1801h.pcr @01:11:23.08 Building PCR Slug from ASV with MM3 mix, PP6 primer @01:11:23.10 Priming with carrier @01:11:23.12 ATV.dial ARV @01:11:23.84 ARV.dial :carrier @01:11:24.19 AS.seek 0.5ml @01:11:49.76 AFV.select :carrierSolution @01:11:49.89 delay 2 seconds @01:11:51.91 AS.reconfigure ASconfig @01:11:51.98 AS.seek 1ml @01:12:21.00 delay 1 second @01:12:22.02 AFV.select :holdingCoil @01:12:22.13 delay 1 second @01:12:23.14 ATV.dial :waste @01:12:24.80 AS.reconfigure ASnorm @01:12:24.86 AS.seek :empty @01:13:15.61 AFV.select :carrierSolution @01:13:15.73 delay 2 seconds @01:13:17.75 AS.reconfigure ASconfig @01:13:17.82 AS.seek 0.5ml @01:13:46.89 delay 1 second @01:13:47.91 AFV.select :holdingCoil @01:13:48.02 Building Slug @01:13:48.03 ATV.dial :ARV @01:13:49.67 delay 2 seconds @01:13:51.69 ARV.dial :air1 @01:13:52.20 delay 2 seconds @01:13:54.27 AS.reconfigure ASslow2 @01:13:54.34 AS.seek 0.506ml @01:14:00.70 delay 2 seconds @01:14:02.72 ARV.dial :MM3 @01:14:03.78 delay 2 seconds @01:14:05.81 Adding MM3 @01:14:05.90 AS.seek 0.524ml @01:14:24.23 delay 25 seconds @01:14:49.25 ARV.dial :PP6 @01:14:49.66 Adding PP6 @01:14:49.67 delay 2 seconds @01:14:51.75 AS.seek 0.53ml @01:14:58.05 delay 10 seconds @01:15:08.08 ARV.dial :air1 @01:15:08.93 adding ARV air @01:15:08.94 delay 2 seconds @01:15:11.02 AS.seek 0.536ml @01:15:17.37 delay 5 seconds @01:15:22.39 ARV.dial :carrier @01:15:22.79 delay 7 seconds @01:15:29.88 AS.seek 0.594ml @01:16:28.34 delay 5 seconds @01:16:33.36 ATV.dial :ASV @01:16:33.76 delay 2 seconds @01:16:35.84 AS.seek 0.6ml @01:16:42.06 delay 4 seconds @01:16:46.08 ATV.dial ARV @01:16:46.64 delay 2 seconds @01:16:48.66 ARV.dial :carrier @01:16:48.68 delay 2 seconds @01:16:50.76 AS.seek 0.65ml @01:17:41.22 delay 4 seconds @01:17:45.24 ATV.dial PCR @01:17:45.86 ASV.dial :waste1 @01:17:45.88 ARV.dial :air1 @01:17:46.83 Positioning Slug by 0.324ml @01:17:46.92 AS.seek 0.326ml @01:23:13.16 delay 5 seconds @01:23:18.18 ATV.dial ARV @01:23:18.97 ASV.dial :PCRlock @01:23:19.36 delay 5 seconds @01:23:24.39 Running Tt2 Profile60C300 thermocycling @01:23:24.40 PCR.program Profile60C300 @01:23:24.55 Profile60C300 should complete in < 1:45:21 @01:23:24.56 PCR.run 2:16:41.25 @02:44:09.14 PCR.off @02:44:10.66 PCR for Tt2 completed -- closed /var/log/esp/Tt218aug1801h.pcr @02:44:10.67 Full MFB Decontamination @02:44:10.70 Decon extraction column and decon AIV @02:44:10.73 SPE.hold 2:46:40,55C @02:44:10.79 ASV.dial :SPEdelivery @02:44:11.45 delay 20 seconds @02:44:31.48 AFV.select :carrierSolution @02:44:31.59 delay 2 seconds @02:44:33.61 AS.reconfigure ASconfig @02:44:33.67 AS.seek 0.5ml @02:44:43.97 delay 1 second @02:44:44.99 AFV.select :holdingCoil @02:44:45.10 ATV.dial :air1 @02:44:47.07 AS.reconfigure ASslow1 @02:44:47.13 AS.seek 0.52ml @02:44:51.45 ATV.dial :ASV @02:44:53.25 ASV.dial :SPErecovery @02:44:53.94 AS.seek 0.62ml @02:45:14.33 ATV.dial :waste @02:45:16.40 AS.reconfigure ASnorm @02:45:16.46 AS.seek :empty @02:45:48.08 AFV.select :holdingCoil @02:45:48.09 ATV.dial :waste @02:45:48.13 AS.reconfigure ASconfig @02:45:48.19 AS.seek 0ml @02:45:48.48 AFV.select :carrierSolution @02:45:48.60 delay 2 seconds @02:45:51.13 AS.seek 0.5ml @02:46:20.16 delay 1 second @02:46:21.18 AFV.select :holdingCoil @02:46:21.29 ATV.dial :air3 @02:46:22.03 AS.reconfigure ASslow1 @02:46:22.10 AS.seek 0.52ml @02:46:26.41 ATV.dial :air3 @02:46:26.51 AS.seek 0.53ml @02:46:28.80 delay 2 seconds @02:46:30.82 ATV.dial :bleach @02:46:31.34 AS.seek 0.71ml @02:47:07.78 delay 3 seconds @02:47:10.80 ATV.dial :air3 @02:47:11.41 delay 3 seconds @02:47:14.49 AS.seek 0.735ml @02:47:19.78 delay 3 seconds @02:47:22.80 ATV.dial :bleach @02:47:23.47 AS.seek 0.72ml @02:47:26.80 delay 3 seconds @02:47:29.83 ATV.dial ASV @02:47:30.94 ASV.dial :SPErecovery @02:47:31.03 AS.seek 0.365ml @02:48:42.69 ATV.dial :waste @02:48:44.59 AS.reconfigure ASnorm @02:48:44.65 AS.seek :empty @02:49:03.44 ATV.dial :air3 @02:49:04.14 AS.reconfigure ASslow1 @02:49:04.20 AS.seek 0.009ml @02:49:06.53 delay 2 seconds @02:49:08.55 ATV.dial :bleach @02:49:09.05 AS.seek 0.579ml @02:51:03.94 delay 3 seconds @02:51:06.96 ATV.dial :air3 @02:51:07.53 delay 3 seconds @02:51:10.61 AS.seek 0.604ml @02:51:15.92 delay 3 seconds @02:51:18.94 ATV.dial :bleach @02:51:19.58 AS.seek 0.589ml @02:51:22.89 delay 3 seconds @02:51:25.91 ATV.dial :ASV @02:51:26.97 ASV.dial :wasteR @02:51:27.36 AS.seek 0ml @02:53:26.08 delay 1:00 @02:54:26.10 ATV.dial ASV @02:54:26.12 AFV.select :carrierSolution @02:54:26.24 delay 2 seconds @02:54:28.26 AS.reconfigure ASconfig @02:54:28.32 AS.seek 1ml @02:55:26.08 delay 1 second @02:55:27.10 AFV.select :holdingCoil @02:55:27.21 delay 1 second @02:55:28.22 ASV.dial :wasteR @02:55:28.24 AS.reconfigure ASslow1 @02:55:28.32 AS.seek 0ml @02:58:49.69 delay 10 seconds @02:58:59.73 ATV.dial :air3 @02:59:01.24 AS.seek 0.01ml @02:59:03.57 delay 2 seconds @02:59:05.59 ATV.dial :bleach @02:59:06.24 AS.seek 0.58ml @03:01:01.16 delay 3 seconds @03:01:04.18 ATV.dial :air3 @03:01:04.74 delay 3 seconds @03:01:07.82 AS.seek 0.605ml @03:01:13.19 delay 3 seconds @03:01:16.21 ATV.dial :bleach @03:01:16.83 AS.seek 0.59ml @03:01:20.14 delay 3 seconds @03:01:23.16 ATV.dial ASV @03:01:24.22 ASV.dial :SPEdelivery @03:01:24.59 AS.seek 0ml @03:03:23.54 delay 1:00 @03:04:23.56 AFV.select :carrierSolution @03:04:23.68 delay 2 seconds @03:04:25.70 AS.reconfigure ASconfig @03:04:25.76 AS.seek 1ml @03:05:23.52 delay 1 second @03:05:24.54 AFV.select :holdingCoil @03:05:24.65 delay 1 second @03:05:25.66 ATV.dial ASV @03:05:25.67 ASV.dial :SPEdelivery @03:05:25.71 AS.reconfigure ASslow1 @03:05:25.78 AS.seek 0ml @03:08:47.16 delay 10 seconds @03:08:57.19 ATV.dial :air2 @03:08:58.05 AS.reconfigure ASslow0 @03:08:58.11 AS.seek 0.8ml @03:10:19.43 delay 3 seconds @03:10:22.45 ATV.dial :ASV @03:10:23.44 ASV.dial :SPEdelivery @03:10:23.46 AS.reconfigure ASslow1 @03:10:23.54 AS.seek 0.03ml @03:12:58.68 delay 20 seconds @03:13:18.72 ATV.dial :waste,via: :air3 @03:13:20.45 AS.reconfigure ASconfig @03:13:20.51 AS.seek :empty @03:13:22.60 SPE.coast @03:13:22.69 Removing Bleach from Mixing Coil @03:13:22.70 AFV.select :carrierSolution @03:13:22.82 delay 2 seconds @03:13:24.84 AS.seek 0.5ml @03:13:53.94 delay 1 second @03:13:54.96 AFV.select :holdingCoil @03:13:55.07 ATV.dial :ASV @03:13:56.79 ASV.dial :SPErecovery @03:13:57.44 AS.reconfigure ASslow1 @03:13:57.50 AS.seek 0.73ml @03:14:44.02 ASV.dial :wasteD @03:14:44.72 AS.reconfigure ASnorm @03:14:44.78 AS.seek 0ml @03:15:21.90 AFV.select :carrierSolution @03:15:22.02 delay 2 seconds @03:15:24.04 AS.reconfigure ASconfig @03:15:24.10 AS.seek 0.5ml @03:15:53.18 delay 1 second @03:15:54.20 AFV.select :holdingCoil @03:15:54.31 ATV.dial ASV @03:15:54.32 ASV.dial :SPErecovery @03:15:54.96 AS.reconfigure ASslow0 @03:15:55.03 AS.seek 0.3ml @03:16:15.46 ATV.dial :air2 @03:16:16.50 AS.reconfigure ASslow1 @03:16:16.56 AS.seek 0.32ml @03:16:20.90 ATV.dial ASV @03:16:21.94 AS.reconfigure ASslow0 @03:16:22.00 AS.seek 0.57ml @03:16:47.46 ASV.dial :wasteD @03:16:48.06 AS.reconfigure ASnorm @03:16:48.12 AS.seek 0ml @03:17:17.21 AFV.select :carrierSolution @03:17:17.33 delay 2 seconds @03:17:19.35 AS.reconfigure ASconfig @03:17:19.41 AS.seek 0.5ml @03:17:48.49 delay 1 second @03:17:49.51 AFV.select :holdingCoil @03:17:49.62 ATV.dial ASV @03:17:49.63 ASV.dial :SPErecovery @03:17:50.29 AS.reconfigure ASslow0 @03:17:50.35 AS.seek 0.3ml @03:18:10.77 ATV.dial :air2 @03:18:11.82 AS.reconfigure ASslow1 @03:18:11.88 AS.seek 0.32ml @03:18:16.16 ATV.dial ASV @03:18:17.21 AS.reconfigure ASslow0 @03:18:17.27 AS.seek 0.57ml @03:18:42.74 ASV.dial :wasteD @03:18:43.35 AS.reconfigure ASnorm @03:18:43.41 AS.seek 0ml @03:19:12.49 ATV.dial :air2 @03:19:13.47 AS.reconfigure ASslow0 @03:19:13.53 AS.seek 0.3ml @03:19:44.07 ATV.dial :ASV @03:19:45.05 ASV.dial :wasteD @03:19:45.07 AS.reconfigure ASconfig @03:19:45.14 AS.seek 0.05ml @03:19:59.82 ATV.dial :waste @03:20:01.69 AS.seek 0ml @03:20:04.91 ASV.dial :SPEdelivery @03:20:05.91 deconLysateINT @03:20:05.92 ATV.dial :air3 @03:20:06.61 AS.reconfigure ASslow1 @03:20:06.67 AS.seek 0.01ml @03:20:09.02 delay 2 seconds @03:20:11.04 ATV.dial :bleach @03:20:11.51 AS.seek 0.96ml @03:23:22.85 delay 3 seconds @03:23:25.87 ATV.dial :air3 @03:23:26.43 delay 3 seconds @03:23:29.51 AS.seek 0.985ml @03:23:34.80 delay 3 seconds @03:23:37.82 ATV.dial :bleach @03:23:38.47 AS.seek 0.97ml @03:23:41.77 delay 3 seconds @03:23:44.80 ATV.dial AIV @03:23:46.15 AIV.dial :coilToMFB @03:23:46.17 AWV.select :waste @03:23:46.19 AFV.select :carrierSolution @03:23:46.32 delay 2 seconds @03:23:48.34 AS.reconfigure ASconfig @03:23:48.41 AS.seek 1ml @03:23:50.45 delay 1 second @03:23:51.47 AFV.select :holdingCoil @03:23:51.58 delay 1 second @03:23:52.60 AS.reconfigure ASslow0 @03:23:52.66 AS.seek :empty @03:25:33.62 AFV.select :carrierSolution @03:25:33.74 delay 2 seconds @03:25:35.76 AS.reconfigure ASconfig @03:25:35.83 AS.seek 1ml @03:26:33.65 delay 1 second @03:26:34.67 AFV.select :holdingCoil @03:26:34.78 delay 1 second @03:26:35.80 AS.reconfigure ASslow0 @03:26:35.86 AS.seek :empty @03:28:16.84 AFV.select :carrierSolution @03:28:16.96 delay 2 seconds @03:28:18.98 AS.reconfigure ASconfig @03:28:19.05 AS.seek 1ml @03:29:16.87 delay 1 second @03:29:17.89 AFV.select :holdingCoil @03:29:18.00 delay 1 second @03:29:19.02 AS.reconfigure ASslow0 @03:29:19.08 AS.seek :empty @03:31:00.06 ATV.dial :air2 @03:31:00.74 AS.reconfigure ASslow1 @03:31:00.81 AS.seek 0.009ml @03:31:03.24 AS.reconfigure ASslow0 @03:31:03.30 AS.seek 1ml @03:32:43.28 ATV.dial AIV @03:32:44.05 AS.seek 0.02ml @03:34:22.96 ATV.dial :waste @03:34:24.72 AS.seek 0ml @03:34:27.03 AWV.select :air @03:34:27.20 Cleaning PCR @03:34:27.21 AFV.select :carrierSolution @03:34:27.34 delay 2 seconds @03:34:29.36 AS.reconfigure ASconfig @03:34:29.42 AS.seek 0.5ml @03:34:58.49 delay 1 second @03:34:59.51 AFV.select :holdingCoil @03:34:59.62 ASV.dial :waste1 @03:34:59.99 ATV.dial :air3 @03:35:00.68 AS.reconfigure ASslow1 @03:35:00.74 AS.seek 0.51ml @03:35:03.01 delay 2 seconds @03:35:05.03 ATV.dial :bleach @03:35:05.49 AS.seek 0.81ml @03:36:06.04 delay 3 seconds @03:36:09.06 ATV.dial :air3 @03:36:09.62 delay 3 seconds @03:36:12.70 AS.seek 0.835ml @03:36:18.04 delay 3 seconds @03:36:21.06 ATV.dial :bleach @03:36:21.68 AS.seek 0.82ml @03:36:25.00 delay 3 seconds @03:36:28.02 ARV.dial :waste @03:36:28.64 delay 3 seconds @03:36:31.66 ATV.dial :ARV @03:36:32.56 AS.reconfigure ASconfig @03:36:32.62 AS.seek 0.47ml @03:36:53.01 AFV.select :carrierSolution @03:36:53.13 delay 2 seconds @03:36:55.15 AS.seek 1ml @03:37:25.95 delay 1 second @03:37:26.97 AFV.select :holdingCoil @03:37:27.08 delay 1 second @03:37:28.10 AS.reconfigure ASfast @03:37:28.17 AS.seek 0ml @03:37:55.53 AFV.select :carrierSolution @03:37:55.65 delay 2 seconds @03:37:57.67 AS.reconfigure ASconfig @03:37:57.73 AS.seek 1ml @03:38:55.53 delay 1 second @03:38:56.55 AFV.select :holdingCoil @03:38:56.66 delay 1 second @03:38:57.68 AS.reconfigure ASfast @03:38:57.74 AS.seek 0ml @03:39:25.09 AFV.select :carrierSolution @03:39:25.21 delay 2 seconds @03:39:27.23 AS.reconfigure ASconfig @03:39:27.28 AS.seek 1ml @03:40:25.04 delay 1 second @03:40:26.06 AFV.select :holdingCoil @03:40:26.17 delay 1 second @03:40:27.19 AS.reconfigure ASfast @03:40:27.24 AS.seek 0ml @03:40:54.59 ARV.dial between carrier and air1 @03:40:55.09 ATV.dial :air3 @03:40:56.37 AS.reconfigure ASslow1 @03:40:56.43 AS.seek 0.01ml @03:40:58.78 delay 2 seconds @03:41:01.29 ATV.dial :bleach @03:41:01.94 AS.seek 0.81ml @03:43:43.09 delay 3 seconds @03:43:46.11 ATV.dial :air3 @03:43:46.65 delay 3 seconds @03:43:49.73 AS.seek 0.835ml @03:43:54.96 delay 3 seconds @03:43:57.99 ATV.dial :bleach @03:43:58.61 AS.seek 0.82ml @03:44:01.92 delay 3 seconds @03:44:04.94 ATV.dial PCR @03:44:05.35 AS.reconfigure ASconfig @03:44:05.41 AS.seek :empty @03:44:52.87 PCR.program :bleachProfile @03:44:52.93 PCR program should complete in < 2:45 @03:44:52.94 PCR.run 10:00 @03:47:57.81 PCR.off @03:47:57.84 AFV.select :carrierSolution @03:47:57.96 delay 2 seconds @03:47:59.98 AS.seek 1ml @03:48:57.83 delay 1 second @03:48:58.85 AFV.select :holdingCoil @03:48:58.96 delay 1 second @03:48:59.98 AS.reconfigure ASfast @03:49:00.04 AS.seek 0ml @03:49:27.39 delay 3 seconds @03:49:30.41 AFV.select :carrierSolution @03:49:30.52 delay 2 seconds @03:49:32.54 AS.reconfigure ASconfig @03:49:32.60 AS.seek 1ml @03:50:30.34 delay 1 second @03:50:31.36 AFV.select :holdingCoil @03:50:31.47 delay 1 second @03:50:32.49 AS.reconfigure ASfast @03:50:32.56 AS.seek 0ml @03:51:00.11 delay 3 seconds @03:51:03.13 AFV.select :carrierSolution @03:51:03.24 delay 2 seconds @03:51:05.26 AS.reconfigure ASconfig @03:51:05.33 AS.seek 1ml @03:52:03.12 delay 1 second @03:52:04.14 AFV.select :holdingCoil @03:52:04.25 delay 1 second @03:52:05.27 AS.reconfigure ASfast @03:52:05.33 AS.seek 0ml @03:52:32.67 delay 3 seconds @03:52:35.69 AFV.select :carrierSolution @03:52:35.80 delay 2 seconds @03:52:37.82 AS.reconfigure ASconfig @03:52:37.88 AS.seek 1ml @03:53:35.65 delay 1 second @03:53:36.67 AFV.select :holdingCoil @03:53:36.78 delay 1 second @03:53:37.80 AS.reconfigure ASfast @03:53:37.86 AS.seek 0ml @03:54:05.22 delay 3 seconds @03:54:08.24 AFV.select :carrierSolution @03:54:08.35 delay 2 seconds @03:54:10.37 AS.reconfigure ASconfig @03:54:10.44 AS.seek 1ml @03:55:08.22 delay 1 second @03:55:09.24 AFV.select :holdingCoil @03:55:09.35 delay 1 second @03:55:10.37 AS.reconfigure ASnorm @03:55:10.43 AS.seek 0ml @03:56:01.14 AFV.select :carrierSolution @03:56:01.25 delay 2 seconds @03:56:03.27 AS.reconfigure ASconfig @03:56:03.32 AS.seek 1ml @03:57:01.09 delay 1 second @03:57:02.11 AFV.select :holdingCoil @03:57:02.22 delay 1 second @03:57:03.23 ATV.dial :waste @03:57:04.45 AS.reconfigure ASnorm @03:57:04.51 AS.seek 0.5ml @03:57:30.11 ATV.dial :waste2 @03:57:31.45 AS.seek 0ml @03:57:57.00
Asleep after completing 18tun assays @03:57:57.02 Mission runtime was 14:04:02.855339 @03:57:57.04 GoodNews.email "{1 => 3, 2 => 0, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\nCan@03:53:56, 16.7C, 49% humidity, 14.6psia, 12.065V, 0.430A, 0.424A avg, 5.19W",Subject: "Asleep after completing 18tun assays" @03:58:04.78 Session Ended @12:41:31.46CEST21-Aug-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @12:41:33.12 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @12:41:33.38 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @12:41:37.71 Polling Can every 10:00 @12:41:39.46 SPE.coast @12:41:39.51 SPE.configure SPEconfig @12:41:39.59 AS.reconfigure ASconfig @12:41:39.63 AS.hold @12:41:39.68 AS.configure ASconfig @12:41:39.76 SS.reconfigure SSconfig @12:41:39.80 SS.hold @12:41:39.85 SS.configure SSconfig @12:41:39.93 CC.reconfigure CCconfig @12:41:39.96 CC.hold @12:41:40.02 CC.configure CCconfig @12:41:40.10 CS.reconfigure CSconfig @12:41:40.14 CS.hold @12:41:40.21 CS.configure CSconfig @12:41:40.62 PC.reconfigure PCconfig @12:41:40.66 PC.hold @12:41:40.72 PC.configure PCconfig @12:41:40.80 PS.configure PSconfig @12:41:40.90 Forearm.reconfigure LooseForearm @12:41:40.93 Forearm.hold @12:41:40.99 Forearm.configure LooseForearm @12:41:41.07 Elbow.reconfigure LooseElbow @12:41:41.11 Elbow.hold @12:41:41.17 Elbow.configure LooseElbow @12:41:41.27 SC.reconfigure SCloose @12:41:41.30 SC.hold @12:41:41.36 SC.configure SCloose @12:41:41.44 SE.reconfigure SEconfig @12:41:41.48 SE.hold @12:41:41.54 SE.configure SEconfig @12:41:41.58 CH.coast @12:41:41.63 CH.configure CHconfig @12:41:41.65 PH.coast @12:41:41.70 PH.configure PHconfig @12:41:41.74 PCR.off @12:41:41.77 PCR.pidtemp= 50,0.1,0,300,25,25 @12:41:41.80 PCR.caltemp= 2.7073,0.0373,0 @12:41:41.83 All dwarf controllers running firmware version 3.75 @12:41:41.95 Cleanly exited at 12:39:36CEST21-Aug-18 @12:41:41.98 Loading /home/esp/esp2/mission/18tun1.rb mission @12:41:42.02 RuntimeError in MAIN -- Mission's startTube should be 3, not 2 @12:41:42.04 Waiting to Resume from Checkpoint "Loading /home/esp/esp2/mission/18tun1.rb mission" @12:41:42.05 BadNews.email "Mission's startTube should be 3, not 2",Subject: "RuntimeError in MAIN" @12:44:49.90 Session Begun @12:46:54.78 -> ls @12:46:54.80 NameError in mwj -- undefined local variable or method `ls' for # @12:47:03.36 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @12:49:43.19 Session Begun @12:49:47.38 -> showlog 100 @12:51:40.21 -> exit @12:51:40.23 Session Ended @13:11:17.39CEST21-Aug-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @13:11:19.50 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @13:11:19.76 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @13:11:25.69 Polling Can every 10:00 @13:11:27.88 SPE.coast @13:11:27.92 SPE.configure SPEconfig @13:11:28.01 AS.reconfigure ASconfig @13:11:28.04 AS.hold @13:11:28.11 AS.configure ASconfig @13:11:28.20 SS.reconfigure SSconfig @13:11:28.23 SS.hold @13:11:28.30 SS.configure SSconfig @13:11:28.38 CC.reconfigure CCconfig @13:11:28.46 CC.hold @13:11:28.56 CC.configure CCconfig @13:11:28.72 CS.reconfigure CSconfig @13:11:28.80 CS.hold @13:11:28.91 CS.configure CSconfig @13:11:29.47 PC.reconfigure PCconfig @13:11:29.51 PC.hold @13:11:29.57 PC.configure PCconfig @13:11:29.65 PS.configure PSconfig @13:11:29.75 Forearm.reconfigure LooseForearm @13:11:29.79 Forearm.hold @13:11:29.85 Forearm.configure LooseForearm @13:11:29.93 Elbow.reconfigure LooseElbow @13:11:29.97 Elbow.hold @13:11:30.01 Elbow.configure LooseElbow @13:11:30.17 SC.reconfigure SCloose @13:11:30.23 SC.hold @13:11:30.33 SC.configure SCloose @13:11:30.49 SE.reconfigure SEconfig @13:11:30.57 SE.hold @13:11:30.67 SE.configure SEconfig @13:11:30.73 CH.coast @13:11:30.83 CH.configure CHconfig @13:11:30.89 PH.coast @13:11:30.95 PH.configure PHconfig @13:11:30.99 PCR.off @13:11:31.01 PCR.pidtemp= 50,0.1,0,300,25,25 @13:11:31.04 PCR.caltemp= 2.7073,0.0373,0 @13:11:31.07 All dwarf controllers running firmware version 3.75 @13:11:31.20 Loading /home/esp/esp2/mission/18tun1.rb mission @13:11:31.26 Preparing to sleep until +0:00:15 @13:11:31.27 GoodNews.email "Starting 18tun1 w/pucks in tube 2\n{}\nCan@13:11:25, 17.0C, 71% humidity, 14.7psia, 12.006V, 0.651A, 0.651A avg, 7.82W",Subject: "18tun1 begun" @13:11:31.35 AS.coast @13:11:31.37
Asleep til +0:00:15 @13:11:31.42 Continuing at 13:11 @13:11:31.43 SS.coast @13:11:31.50 CC.coast @13:11:31.53 CS.coast @13:11:31.56 PC.coast @13:11:31.60 Forearm.coast @13:11:31.64 Elbow.coast @13:11:31.70 SC.coast @13:11:31.76 SE.coast @13:11:32.26 Session Begun @13:11:32.38 Gate.power :core,:OFF @13:11:32.40 Gate.power :raw,:OFF @13:11:32.42 Shutdown @13:11:37.27 -> showlog 100 @13:11:38.40 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:106\n{}\nCan@13:11:31, 17.0C, 70% humidity, 14.7psia, 12.006V, 0.651A, 0.645A avg, 7.82W",Subject: "Asleep until 13:11" @13:11:46.23
Powering core bus @13:11:51.08 Gate.power :raw,:ON @13:11:51.69 SPE.coast @13:11:51.75 SPE.configure SPEconfig @13:11:51.85 AS.configure ASconfig @13:11:51.96 SS.configure SSconfig @13:11:52.06 CC.configure CCconfig @13:11:52.16 CS.configure CSconfig @13:11:52.27 PC.configure PCconfig @13:11:52.37 PS.configure PSconfig @13:11:52.49 Forearm.configure LooseForearm @13:11:52.59 Elbow.configure LooseElbow @13:11:52.74 SC.configure SCloose @13:11:52.84 SE.configure SEconfig @13:11:52.90 CH.coast @13:11:52.96 CH.configure CHconfig @13:11:53.00 PH.coast @13:11:53.07 PH.configure PHconfig @13:11:53.12 PCR.off @13:11:53.16 PCR.pidtemp= 50,0.1,0,300,25,25 @13:11:53.19 PCR.caltemp= 2.7073,0.0373,0 @13:11:53.23 All dwarf controllers running firmware version 3.75 @13:11:53.35 Valve.reset @13:11:53.53 PCR.off @13:11:53.56 PCR.pidtemp= 50,0.1,0,300,25,25 @13:11:53.60 PCR.caltemp= 2.7073,0.0373,0 @13:11:53.69 Intake.select :close @13:11:53.71 Exhaust.select :close @13:11:53.75 CTV.dial :air @13:11:53.92 PSV.dial :air @13:11:54.07 CSV.dial :waste1 @13:11:54.10
Forearm.reconfigure LooseForearm @13:11:54.13 PS.reconfigure PSconfig @13:11:54.26
Forearm.home.seek :retract @13:11:54.65 PS.home.seek 0ml @13:11:54.77 SSV.dial :spit,avoiding: :exhaust @13:11:54.92 CS.reconfigure CSfast @13:11:54.95 SS.reconfigure SSconfig @13:11:55.05 CS.home.seek 0ml @13:11:55.08 SS.home.seek 0ml @13:11:55.39 Hand.close @13:11:55.45
Elbow.jog 1500 @13:11:56.03 Elbow.reconfigure LooseElbow @13:11:56.09 Elbow.home.seek :clear @13:12:00.80 SE.reconfigure SEconfig @13:12:00.86 SE.home.seek :down @13:12:01.49 SC.reconfigure SCsloppy @13:12:01.55 SC.home.seek :initial @13:12:03.91 PS.coast @13:12:05.55
Forearm.seek :retract @13:12:05.64 Elbow.seek :hammer @13:12:06.82 Forearm.reconfigure TightForearm @13:12:06.89 Forearm.seek 5 + 350 counts @13:12:07.89 Forearm.seek :retract @13:12:09.03 PC.reconfigure PCconfig @13:12:09.06
CC.reconfigure CCconfig @13:12:09.15 PC.home.seek :open @13:12:09.19
CC.home.seek :open @13:12:12.49 Phase [mbaPhase] @13:12:12.51 GoodNews.email "At 13:12:12 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:106\n{}\nCan@13:12:12, 17.1C, 71% humidity, 14.7psia, 11.977V, 0.771A, 0.771A avg, 9.23W",Subject: "Started 18tun1 phase mbaPhase" @13:12:12.54CEST21-Aug-18
==================> STARTED MBAsuperphase (phase #1) <=== @13:12:12.56 Starting initialPurge @13:12:12.60 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:12:12.68 Hand.open @13:12:13.50 Elbow.reconfigure TightElbow @13:12:13.56 Elbow.seek FlushPuck::Garage @13:12:15.47 Elbow.reconfigure LooseElbow @13:12:15.59 Forearm.reconfigure LooseForearm @13:12:15.67 Forearm.seek :garage @13:12:16.68 Hand.close @13:12:17.63 Forearm.seek :retract @13:12:18.64 Elbow.reconfigure TightElbow @13:12:18.70 Elbow.seek CC @13:12:21.52 Elbow.reconfigure LooseElbow @13:12:21.64 Forearm.reconfigure TightForearm @13:12:21.70 Forearm.seek CC @13:12:22.95 CC.seek :closed @13:12:27.32 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.31 @13:12:27.40 Hand.open @13:12:28.20 Forearm.reconfigure LooseForearm @13:12:28.26 Forearm.seek :retract @13:12:29.27 Flushing the Collection Valve::Manifold @13:12:29.42 CSV.dial CSR @13:12:31.00 Pulling 5ml of CSR.flush @13:12:31.01 CSR.select :flush @13:12:31.15 CS.reconfigure CSconfig @13:12:31.21 CS.seek 5ml @13:12:45.14 delay 5 seconds @13:12:50.16 CSR.select :air @13:12:50.34 CS.reconfigure CSfast @13:12:50.40 CS.seek 7.5ml @13:12:57.86 CSV.dial :waste @13:12:59.38 CS.reconfigure CSconfig @13:12:59.45 CS.seek 0ml @13:13:19.58 Purging external Intake @13:13:19.71 CSV.dial :flush @13:13:20.22 CS.reconfigure CSfast @13:13:20.28 CS.seek 10ml @13:13:46.99 delay 5 seconds @13:13:52.01 CSV.dial CBV @13:13:52.05
CBV.dial CSV @13:13:52.94 CTV.dial :intake @13:13:53.83 Intake.select :open @13:13:53.95 CS.seek 0ml @13:14:21.86 Intake.select :close @13:14:21.97 CTV.dial :air @13:14:22.86 Flushing upper manifold @13:14:22.99 CSV.dial :flush @13:14:23.86 CS.seek 5ml @13:14:36.76 Session Begun @13:14:37.80
delay 5 seconds @13:14:42.82 CSV.dial :air @13:14:43.39 CS.seek 7ml @13:14:49.49 CSV.dial CBV @13:14:49.52
CBV.dial CSV @13:14:50.74 CTV.dial :air @13:14:50.76 CS.seek 0ml @13:15:09.80 delay 7 seconds @13:15:16.83 CS.seek 3ml @13:15:25.58 CSV.dial :waste @13:15:26.14 CS.seek 0ml @13:15:35.12 CSV.dial :flush @13:15:36.04 CS.seek 3ml @13:15:44.74 delay 5 seconds @13:15:49.76 CSV.dial :air @13:15:50.35 CS.seek 5ml @13:15:56.61 CSV.dial :air @13:15:56.63 CS.seek 0ml @13:16:10.46 delay 7 seconds @13:16:17.50 CS.seek 3ml @13:16:26.14 CSV.dial :waste @13:16:27.03 CS.seek 0ml @13:16:35.68 Garaging FlushPuck in Collection Clamp @13:16:35.78 Elbow.reconfigure TightElbow @13:16:35.84 Elbow.seek CC @13:16:36.06 Elbow.reconfigure LooseElbow @13:16:36.18 Forearm.seek CC @13:16:37.20 Hand.close @13:16:38.01 CC.seek :open @13:16:42.41 Forearm.seek :retract @13:16:43.40 Elbow.reconfigure TightElbow @13:16:43.47 Elbow.seek :garage @13:16:46.60 Elbow.reconfigure LooseElbow @13:16:46.72 Forearm.reconfigure TightForearm @13:16:46.79 Forearm.seek :garage @13:16:47.99 Hand.open @13:16:48.82 Forearm.reconfigure LooseForearm @13:16:48.89 Forearm.seek :retract @13:16:50.00 Starting mfb @13:16:50.02 pcrBAC sampling at most 1500ml, wcr at most 1500ml @13:16:50.07 BACfans.email "Started mfb in tube 2 at 13:16:50CEST21-Aug-18\n{}\nCan@13:12:12, 17.1C, 71% humidity, 14.7psia, 11.977V, 0.771A, 0.771A avg, 9.23W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @13:16:50.13
Loading sh1 puck from tube 2 into Collection Clamp @13:16:50.29 Readying the MFB @13:16:50.38 Homing MFB Syringe @13:16:50.40 AFV.select :holdingCoil @13:16:50.64 ATV.dial :air2 @13:16:50.77
SC.reconfigure SCloose @13:16:51.01 Elbow.reconfigure TightElbow @13:16:51.08 Elbow.seek 2 @13:16:51.93 AS.reconfigure ASconfig @13:16:52.00 AS.home.seek :home @13:16:53.34
Forearm.reconfigure TightForearm @13:16:53.42 Forearm.seek 2 + 700 counts @13:16:54.48 SE.reconfigure SEpick @13:16:54.58 SE.seek beamBreak @13:16:57.73 SE.configure SEconfig @13:16:57.91 Elbow.seek 2 @13:16:58.10 AFV.select :carrierSolution @13:16:58.24
Forearm.seek 2 + 300 counts @13:16:58.31 delay 2 seconds @13:16:58.63
Forearm.reconfigure LooseForearm @13:16:58.71 Hand.close @13:16:59.52 SE.seek :down @13:16:59.76
Forearm.seek :retract @13:17:00.34 AS.seek 1ml @13:17:00.74
Elbow.seek CC @13:17:02.24 SE.coast @13:17:02.76
Elbow.reconfigure LooseElbow @13:17:02.89 Forearm.reconfigure TightForearm @13:17:02.96 Forearm.seek CC @13:17:04.23 CC.seek :closed @13:17:08.59 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.42 @13:17:08.61 Hand.open @13:17:09.45 Forearm.reconfigure LooseForearm @13:17:09.52 Forearm.seek :retract @13:17:10.54 SC.coast @13:17:10.60 collectSample 1500ml w/bubble point of 55psi @13:17:10.61 Priming 7ml @13:17:10.63 Gate.power :sampler,:ON @13:17:10.66 Intake.select :close @13:17:10.68 Exhaust.select :close @13:17:10.72 SS.hold @13:17:10.97 Calibrating pressure and temperature sensors @13:17:10.99 CTV.dial :air @13:17:11.03 SSV.dial :spit,avoiding: :exhaust @13:17:11.07 SS.reconfigure SSfast @13:17:11.15 SS.seek 5ml @13:17:14.28 SSV.dial CBV @13:17:14.31
CBV.dial SSV @13:17:15.89 delay 3.8 seconds @13:17:19.82 SSV.dial :spit @13:17:21.37 SS.seek 0ml @13:17:23.97 SSV.dial CBV @13:17:25.53 CTV.dial :intake @13:17:26.43 Waiting for external sampler to finish priming... @13:17:26.44 delayUntil "13:22:10.66" @13:17:53.24 delay 1 second @13:17:54.26 AFV.select :holdingCoil @13:17:54.38 delay 1 second @13:17:55.40 ATV.dial :waste @13:17:56.64 AS.reconfigure ASnorm @13:17:56.71 AS.home.seek :empty @13:18:49.33 ATV.dial :waste @13:18:49.34 ARV.dial between carrier and air1 @13:18:49.46 ASV.dial :waste1 @13:22:10.67 SSV.dial CBV @13:22:10.70
CBV.dial SSV @13:22:10.74 Allowing 40s for 7.0ml of remaining 7.0ml @13:22:10.76 Intake.select :open @13:22:12.96 Intake @29.2psia, vacuum from 20.0 to 24.0psi @13:22:12.98 SS.reconfigure SSinhale.with maxSpeed: 150 @13:22:13.07 SS.seek 7ml @13:22:18.22 Vacuum too high -- waiting up to 40s @13:22:20.37 Intake @26.2psia, vacuum from 19.7 to 23.7psi @13:22:20.39 SS.reconfigure SSinhale.with maxSpeed: 135 @13:22:20.49 SS.seek 7ml @13:22:21.56 Stroke completed -- waiting for pressure to equalize ... @13:22:25.85 Intake.select :close @13:22:26.01 SSV.dial :exhaust @13:22:26.89 Exhaust.select :open @13:22:27.03 SS.reconfigure SSexhale @13:22:27.09 SS.seek 0ml @13:22:30.77 Exhaust.select :close @13:22:30.91 Sampling 1500.0ml @13:22:30.94 Intake.select :close @13:22:30.97 Exhaust.select :close @13:22:31.00 SS.reconfigure SSconfig @13:22:31.09 SS.hold @13:22:31.32 Calibrating pressure sensors @13:22:31.33 CTV.dial :air @13:22:32.71 SSV.dial :spit,avoiding: :exhaust @13:22:33.61 SS.reconfigure SSfast @13:22:33.68 SS.seek 5ml @13:22:36.75 SSV.dial CBV @13:22:36.78
CBV.dial SSV @13:22:38.34 delay 3.8 seconds @13:22:42.26 SSV.dial :spit @13:22:43.82 SS.seek 0ml @13:22:46.49 SSV.dial CBV @13:22:48.02 CTV.dial :intake @13:22:48.93 SSV.dial CBV @13:22:48.96
CBV.dial SSV @13:22:48.98 Allowing 130s for 25.0ml of remaining 1500.0ml @13:22:49.03 Intake.select :open @13:22:51.23 Intake @29.7psia, vacuum from 20.0 to 24.0psi @13:22:51.25 SS.reconfigure SSinhale.with maxSpeed: 135 @13:22:51.34 SS.seek 25ml @13:22:55.12 Vacuum too high -- waiting up to 40s @13:22:56.11 Intake @26.3psia, vacuum from 19.8 to 23.8psi @13:22:56.13 SS.reconfigure SSinhale.with maxSpeed: 122 @13:22:56.22 SS.seek 25ml @13:23:09.31 Vacuum too high -- waiting up to 40s @13:23:19.51 Intake @26.2psia, vacuum from 19.7 to 23.7psi @13:23:19.53 SS.reconfigure SSinhale.with maxSpeed: 110 @13:23:19.63 SS.seek 25ml @13:23:20.06 Stroke completed -- waiting for pressure to equalize ... @13:23:21.94 Intake.select :close @13:23:22.12 SSV.dial :exhaust @13:23:23.00 Exhaust.select :open @13:23:23.14 SS.reconfigure SSexhale @13:23:23.21 SS.seek 0ml @13:23:32.42 Exhaust.select :close @13:23:32.56 SSV.dial CBV @13:23:32.61
CBV.dial SSV @13:23:33.47 Allowing 130s for 25.0ml of remaining 1475.0ml @13:23:33.49 Intake.select :open @13:23:35.69 Intake @30.0psia, vacuum from 20.0 to 24.0psi @13:23:35.71 SS.reconfigure SSinhale.with maxSpeed: 110 @13:23:35.80 SS.seek 25ml @13:23:39.59 Vacuum too high -- waiting up to 40s @13:23:40.35 Intake @26.4psia, vacuum from 19.9 to 23.9psi @13:23:40.36 SS.reconfigure SSinhale.with maxSpeed: 99 @13:23:40.46 SS.seek 25ml @13:23:55.43 Vacuum too high -- waiting up to 40s @13:24:03.12 Intake @26.3psia, vacuum from 19.8 to 23.8psi @13:24:03.14 SS.reconfigure SSinhale.with maxSpeed: 89 @13:24:03.23 SS.seek 25ml @13:24:04.99 Stroke completed -- waiting for pressure to equalize ... @13:24:06.88 Intake.select :close @13:24:07.04 SSV.dial :exhaust @13:24:07.94 Exhaust.select :open @13:24:08.08 SS.reconfigure SSexhale @13:24:08.14 SS.seek 0ml @13:24:17.60 Exhaust.select :close @13:24:17.74 SSV.dial CBV @13:24:17.76
CBV.dial SSV @13:24:19.11 Allowing 130s for 25.0ml of remaining 1450.0ml @13:24:19.13 Intake.select :open @13:24:21.34 Intake @29.8psia, vacuum from 20.0 to 24.0psi @13:24:21.36 SS.reconfigure SSinhale.with maxSpeed: 89 @13:24:21.45 SS.seek 25ml @13:24:25.31 Vacuum too high -- waiting up to 40s @13:24:26.07 Intake @26.5psia, vacuum from 20.0 to 24.0psi @13:24:26.09 SS.reconfigure SSinhale.with maxSpeed: 80 @13:24:26.18 SS.seek 25ml @13:24:43.40 Vacuum too high -- waiting up to 40s @13:24:47.85 Intake @26.5psia, vacuum from 20.0 to 24.0psi @13:24:47.87 SS.reconfigure SSinhale.with maxSpeed: 72 @13:24:47.96 SS.seek 25ml @13:24:51.34 Stroke completed -- waiting for pressure to equalize ... @13:24:53.20 Intake.select :close @13:24:53.36 SSV.dial :exhaust @13:24:54.27 Exhaust.select :open @13:24:54.41 SS.reconfigure SSexhale @13:24:54.47 SS.seek 0ml @13:25:03.47 Exhaust.select :close @13:25:03.61 SSV.dial CBV @13:25:03.63
CBV.dial SSV @13:25:04.55 Allowing 130s for 25.0ml of remaining 1425.0ml @13:25:04.58 Intake.select :open @13:25:06.77 Intake @29.9psia, vacuum from 20.0 to 24.0psi @13:25:06.78 SS.reconfigure SSinhale.with maxSpeed: 72 @13:25:06.88 SS.seek 25ml @13:25:11.97 Vacuum too high -- waiting up to 40s @13:25:12.50 Intake @26.4psia, vacuum from 19.9 to 23.9psi @13:25:12.52 SS.reconfigure SSinhale.with maxSpeed: 65 @13:25:12.61 SS.seek 25ml @13:25:36.27 Stroke completed -- waiting for pressure to equalize ... @13:25:39.37 Intake.select :close @13:25:39.53 SSV.dial :exhaust @13:25:40.44 Exhaust.select :open @13:25:40.58 SS.reconfigure SSexhale @13:25:40.64 SS.seek 0ml @13:25:50.16 Exhaust.select :close @13:25:50.38 SSV.dial CBV @13:25:50.46
CBV.dial SSV @13:25:50.62 Session Ended @13:25:50.71 Errno::EPIPE in mwj's_reader -- Broken pipe @13:25:51.50
Allowing 130s for 25.0ml of remaining 1400.0ml @13:25:51.51 Intake.select :open @13:25:53.70 Intake @29.8psia, vacuum from 20.0 to 24.0psi @13:25:53.72 SS.reconfigure SSinhale.with maxSpeed: 65 @13:25:53.79 SS.seek 25ml @13:26:00.22 Vacuum too high -- waiting up to 40s @13:26:00.74 Intake @26.7psia, vacuum from 20.0 to 24.0psi @13:26:00.76 SS.reconfigure SSinhale.with maxSpeed: 59 @13:26:00.83 SS.seek 25ml @13:26:25.16 Stroke completed -- waiting for pressure to equalize ... @13:26:27.48 Intake.select :close @13:26:27.65 SSV.dial :exhaust @13:26:28.53 Exhaust.select :open @13:26:28.66 SS.reconfigure SSexhale @13:26:28.72 SS.seek 0ml @13:26:37.69 Exhaust.select :close @13:26:37.82 SSV.dial CBV @13:26:37.84
CBV.dial SSV @13:26:38.74 Allowing 130s for 25.0ml of remaining 1375.0ml @13:26:38.75 Intake.select :open @13:26:40.95 Intake @30.1psia, vacuum from 20.0 to 24.0psi @13:26:40.96 SS.reconfigure SSinhale.with maxSpeed: 59 @13:26:41.04 SS.seek 25ml @13:26:50.72 Vacuum too high -- waiting up to 40s @13:26:51.01 Intake @26.5psia, vacuum from 20.0 to 24.0psi @13:26:51.03 SS.reconfigure SSinhale.with maxSpeed: 53 @13:26:51.10 SS.seek 25ml @13:27:15.27 Stroke completed -- waiting for pressure to equalize ... @13:27:16.54 Intake.select :close @13:27:16.70 SSV.dial :exhaust @13:27:17.59 Exhaust.select :open @13:27:17.72 SS.reconfigure SSexhale @13:27:17.78 SS.seek 0ml @13:27:26.77 Exhaust.select :close @13:27:26.90 SSV.dial CBV @13:27:26.92
CBV.dial SSV @13:27:27.81 Allowing 130s for 25.0ml of remaining 1350.0ml @13:27:27.82 Intake.select :open @13:27:30.01 Intake @30.1psia, vacuum from 20.0 to 24.0psi @13:27:30.02 SS.reconfigure SSinhale.with maxSpeed: 53 @13:27:30.10 SS.seek 25ml @13:27:58.15 Session Begun @13:28:01.75 -> showlog 100 @13:28:04.63
Stroke completed -- waiting for pressure to equalize ... @13:28:05.91 Intake.select :close @13:28:06.08 SSV.dial :exhaust @13:28:06.99 Exhaust.select :open @13:28:07.13 SS.reconfigure SSexhale @13:28:07.20 SS.seek 0ml @13:28:16.63 Exhaust.select :close @13:28:16.77 SSV.dial CBV @13:28:16.79
CBV.dial SSV @13:28:17.70 Allowing 130s for 25.0ml of remaining 1325.0ml @13:28:17.73 Intake.select :open @13:28:19.92 Intake @30.1psia, vacuum from 20.0 to 24.0psi @13:28:19.94 SS.reconfigure SSinhale.with maxSpeed: 53 @13:28:20.03 SS.seek 25ml @13:28:53.63 Stroke completed -- waiting for pressure to equalize ... @13:28:54.90 Intake.select :close @13:28:55.06 SSV.dial :exhaust @13:28:55.97 Exhaust.select :open @13:28:56.11 SS.reconfigure SSexhale @13:28:56.17 SS.seek 0ml @13:29:05.61 Exhaust.select :close @13:29:05.75 SSV.dial CBV @13:29:05.77
CBV.dial SSV @13:29:06.69 Allowing 130s for 25.0ml of remaining 1300.0ml @13:29:06.72 Intake.select :open @13:29:08.91 Intake @30.1psia, vacuum from 20.0 to 24.0psi @13:29:08.93 SS.reconfigure SSinhale.with maxSpeed: 53 @13:29:09.02 SS.seek 25ml @13:29:42.63 Stroke completed -- waiting for pressure to equalize ... @13:29:43.90 Intake.select :close @13:29:44.06 SSV.dial :exhaust @13:29:44.97 Exhaust.select :open @13:29:45.11 SS.reconfigure SSexhale @13:29:45.18 SS.seek 0ml @13:29:54.63 Exhaust.select :close @13:29:54.77 SSV.dial CBV @13:29:54.79
CBV.dial SSV @13:29:55.70 Allowing 130s for 25.0ml of remaining 1275.0ml @13:29:55.71 Intake.select :open @13:29:57.92 Intake @30.1psia, vacuum from 20.0 to 24.0psi @13:29:57.94 SS.reconfigure SSinhale.with maxSpeed: 53 @13:29:58.03 SS.seek 25ml @13:30:31.66 Stroke completed -- waiting for pressure to equalize ... @13:30:32.94 Intake.select :close @13:30:33.10 SSV.dial :exhaust @13:30:34.00 Exhaust.select :open @13:30:34.14 SS.reconfigure SSexhale @13:30:34.20 SS.seek 0ml @13:30:43.72 Exhaust.select :close @13:30:43.86 SSV.dial CBV @13:30:43.88
CBV.dial SSV @13:30:44.80 Allowing 130s for 25.0ml of remaining 1250.0ml @13:30:44.83 Intake.select :open @13:30:47.01 Intake @30.1psia, vacuum from 20.0 to 24.0psi @13:30:47.03 SS.reconfigure SSinhale.with maxSpeed: 53 @13:30:47.12 SS.seek 25ml @13:31:21.23 Stroke completed -- waiting for pressure to equalize ... @13:31:22.93 Intake.select :close @13:31:23.11 SSV.dial :exhaust @13:31:24.00 Exhaust.select :open @13:31:24.14 SS.reconfigure SSexhale @13:31:24.21 SS.seek 0ml @13:31:33.48 Exhaust.select :close @13:31:33.62 SSV.dial CBV @13:31:33.64
CBV.dial SSV @13:31:34.55 Allowing 130s for 25.0ml of remaining 1225.0ml @13:31:34.56 Intake.select :open @13:31:36.77 Intake @30.1psia, vacuum from 20.0 to 24.0psi @13:31:36.79 SS.reconfigure SSinhale.with maxSpeed: 53 @13:31:36.89 SS.seek 25ml @13:32:10.72 Stroke completed -- waiting for pressure to equalize ... @13:32:12.60 Intake.select :close @13:32:12.77 SSV.dial :exhaust @13:32:13.67 Exhaust.select :open @13:32:13.81 SS.reconfigure SSexhale @13:32:13.88 SS.seek 0ml @13:32:23.19 Exhaust.select :close @13:32:23.33 SSV.dial CBV @13:32:23.35
CBV.dial SSV @13:32:24.27 Allowing 130s for 25.0ml of remaining 1200.0ml @13:32:24.29 Intake.select :open @13:32:26.49 Intake @30.0psia, vacuum from 20.0 to 24.0psi @13:32:26.51 SS.reconfigure SSinhale.with maxSpeed: 53 @13:32:26.61 SS.seek 25ml @13:33:00.58 Stroke completed -- waiting for pressure to equalize ... @13:33:02.45 Intake.select :close @13:33:02.61 SSV.dial :exhaust @13:33:03.50 Exhaust.select :open @13:33:03.64 SS.reconfigure SSexhale @13:33:03.71 SS.seek 0ml @13:33:12.63 Exhaust.select :close @13:33:12.77 SSV.dial CBV @13:33:12.79
CBV.dial SSV @13:33:13.70 Allowing 130s for 25.0ml of remaining 1175.0ml @13:33:13.73 Intake.select :open @13:33:15.91 Intake @30.0psia, vacuum from 20.0 to 24.0psi @13:33:15.93 SS.reconfigure SSinhale.with maxSpeed: 53 @13:33:16.03 SS.seek 25ml @13:33:50.58 Stroke completed -- waiting for pressure to equalize ... @13:33:52.47 Intake.select :close @13:33:52.63 SSV.dial :exhaust @13:33:53.52 Exhaust.select :open @13:33:53.66 SS.reconfigure SSexhale @13:33:53.73 SS.seek 0ml @13:33:54.46 Session Begun @13:34:03.19
Exhaust.select :close @13:34:03.35 SSV.dial CBV @13:34:03.37
CBV.dial SSV @13:34:04.28 Allowing 130s for 25.0ml of remaining 1150.0ml @13:34:04.30 Intake.select :open @13:34:06.52 Intake @30.1psia, vacuum from 20.0 to 24.0psi @13:34:06.54 SS.reconfigure SSinhale.with maxSpeed: 53 @13:34:06.65 SS.seek 25ml @13:34:40.98 Stroke completed -- waiting for pressure to equalize ... @13:34:42.86 Intake.select :close @13:34:43.03 SSV.dial :exhaust @13:34:43.94 Exhaust.select :open @13:34:44.09 SS.reconfigure SSexhale @13:34:44.16 SS.seek 0ml @13:34:53.53 Exhaust.select :close @13:34:53.68 SSV.dial CBV @13:34:53.70
CBV.dial SSV @13:34:54.62 Allowing 130s for 25.0ml of remaining 1125.0ml @13:34:54.65 Intake.select :open @13:34:56.86 Intake @30.3psia, vacuum from 20.0 to 24.0psi @13:34:56.88 SS.reconfigure SSinhale.with maxSpeed: 53 @13:34:56.99 SS.seek 25ml @13:35:30.62 Stroke completed -- waiting for pressure to equalize ... @13:35:32.32 Intake.select :close @13:35:32.50 SSV.dial :exhaust @13:35:33.41 Exhaust.select :open @13:35:33.56 SS.reconfigure SSexhale @13:35:33.63 SS.seek 0ml @13:35:42.94 Exhaust.select :close @13:35:43.10 SSV.dial CBV @13:35:43.12
CBV.dial SSV @13:35:44.03 Allowing 130s for 25.0ml of remaining 1100.0ml @13:35:44.05 Intake.select :open @13:35:46.27 Intake @30.2psia, vacuum from 20.0 to 24.0psi @13:35:46.29 SS.reconfigure SSinhale.with maxSpeed: 53 @13:35:46.40 SS.seek 25ml @13:36:20.09 Stroke completed -- waiting for pressure to equalize ... @13:36:21.97 Intake.select :close @13:36:22.14 SSV.dial :exhaust @13:36:23.05 Exhaust.select :open @13:36:23.20 SS.reconfigure SSexhale @13:36:23.27 SS.seek 0ml @13:36:32.64 Exhaust.select :close @13:36:32.79 SSV.dial CBV @13:36:32.81
CBV.dial SSV @13:36:33.73 Allowing 130s for 25.0ml of remaining 1075.0ml @13:36:33.75 Intake.select :open @13:36:35.97 Intake @30.2psia, vacuum from 20.0 to 24.0psi @13:36:35.99 SS.reconfigure SSinhale.with maxSpeed: 53 @13:36:36.10 SS.seek 25ml @13:36:59.15 Vacuum too high -- waiting up to 40s @13:36:59.68 Intake @27.2psia, vacuum from 20.0 to 24.0psi @13:36:59.70 SS.reconfigure SSinhale.with maxSpeed: 48 @13:36:59.82 SS.seek 25ml @13:37:11.62 Stroke completed -- waiting for pressure to equalize ... @13:37:12.89 Intake.select :close @13:37:13.06 SSV.dial :exhaust @13:37:13.97 Exhaust.select :open @13:37:14.12 SS.reconfigure SSexhale @13:37:14.20 SS.seek 0ml @13:37:23.59 Exhaust.select :close @13:37:23.74 SSV.dial CBV @13:37:23.76
CBV.dial SSV @13:37:24.67 Allowing 130s for 25.0ml of remaining 1050.0ml @13:37:24.70 Intake.select :open @13:37:26.92 Intake @30.1psia, vacuum from 20.0 to 24.0psi @13:37:26.94 SS.reconfigure SSinhale.with maxSpeed: 48 @13:37:27.05 SS.seek 25ml @13:38:03.95 Stroke completed -- waiting for pressure to equalize ... @13:38:05.22 Intake.select :close @13:38:05.40 SSV.dial :exhaust @13:38:06.30 Exhaust.select :open @13:38:06.46 SS.reconfigure SSexhale @13:38:06.53 SS.seek 0ml @13:38:16.00 Exhaust.select :close @13:38:16.15 SSV.dial CBV @13:38:16.17
CBV.dial SSV @13:38:17.09 Allowing 130s for 25.0ml of remaining 1025.0ml @13:38:17.11 Intake.select :open @13:38:19.33 Intake @30.1psia, vacuum from 20.0 to 24.0psi @13:38:19.35 SS.reconfigure SSinhale.with maxSpeed: 48 @13:38:19.46 SS.seek 25ml @13:38:56.92 Stroke completed -- waiting for pressure to equalize ... @13:38:58.19 Intake.select :close @13:38:58.36 SSV.dial :exhaust @13:38:59.28 Exhaust.select :open @13:38:59.43 SS.reconfigure SSexhale @13:38:59.51 SS.seek 0ml @13:39:08.92 Exhaust.select :close @13:39:09.07 SSV.dial CBV @13:39:09.09
CBV.dial SSV @13:39:10.02 Allowing 130s for 25.0ml of remaining 1000.0ml @13:39:10.04 Intake.select :open @13:39:12.26 Intake @30.2psia, vacuum from 20.0 to 24.0psi @13:39:12.28 SS.reconfigure SSinhale.with maxSpeed: 48 @13:39:12.39 SS.seek 25ml @13:39:50.30 Stroke completed -- waiting for pressure to equalize ... @13:39:51.57 Intake.select :close @13:39:51.74 SSV.dial :exhaust @13:39:52.64 Exhaust.select :open @13:39:52.79 SS.reconfigure SSexhale @13:39:52.86 SS.seek 0ml @13:40:02.12 Exhaust.select :close @13:40:02.27 SSV.dial CBV @13:40:02.29
CBV.dial SSV @13:40:03.22 Allowing 130s for 25.0ml of remaining 975.0ml @13:40:03.24 Intake.select :open @13:40:05.46 Intake @30.1psia, vacuum from 20.0 to 24.0psi @13:40:05.48 SS.reconfigure SSinhale.with maxSpeed: 48 @13:40:05.59 SS.seek 25ml @13:40:42.62 Stroke completed -- waiting for pressure to equalize ... @13:40:43.89 Intake.select :close @13:40:44.06 SSV.dial :exhaust @13:40:44.97 Exhaust.select :open @13:40:45.12 SS.reconfigure SSexhale @13:40:45.20 SS.seek 0ml @13:40:54.09 Exhaust.select :close @13:40:54.24 SSV.dial CBV @13:40:54.26
CBV.dial SSV @13:40:55.19 Allowing 130s for 25.0ml of remaining 950.0ml @13:40:55.22 Intake.select :open @13:40:57.43 Intake @30.1psia, vacuum from 20.0 to 24.0psi @13:40:57.44 SS.reconfigure SSinhale.with maxSpeed: 48 @13:40:57.56 SS.seek 25ml @13:41:35.41 Stroke completed -- waiting for pressure to equalize ... @13:41:36.68 Intake.select :close @13:41:36.85 SSV.dial :exhaust @13:41:37.76 Exhaust.select :open @13:41:37.91 SS.reconfigure SSexhale @13:41:37.98 SS.seek 0ml @13:41:47.22 Exhaust.select :close @13:41:47.37 SSV.dial CBV @13:41:47.39
CBV.dial SSV @13:41:48.31 Allowing 130s for 25.0ml of remaining 925.0ml @13:41:48.33 Intake.select :open @13:41:50.55 Intake @30.2psia, vacuum from 20.0 to 24.0psi @13:41:50.57 SS.reconfigure SSinhale.with maxSpeed: 48 @13:41:50.68 SS.seek 25ml @13:42:27.89 Stroke completed -- waiting for pressure to equalize ... @13:42:29.16 Intake.select :close @13:42:29.34 SSV.dial :exhaust @13:42:30.25 Exhaust.select :open @13:42:30.40 SS.reconfigure SSexhale @13:42:30.48 SS.seek 0ml @13:42:39.89 Exhaust.select :close @13:42:40.04 SSV.dial CBV @13:42:40.06
CBV.dial SSV @13:42:40.98 Allowing 130s for 25.0ml of remaining 900.0ml @13:42:41.00 Intake.select :open @13:42:43.22 Intake @30.0psia, vacuum from 20.0 to 24.0psi @13:42:43.24 SS.reconfigure SSinhale.with maxSpeed: 48 @13:42:43.35 SS.seek 25ml @13:43:08.18 Session Ended @13:43:20.81
Stroke completed -- waiting for pressure to equalize ... @13:43:22.08 Intake.select :close @13:43:22.24 SSV.dial :exhaust @13:43:23.14 Exhaust.select :open @13:43:23.28 SS.reconfigure SSexhale @13:43:23.34 SS.seek 0ml @13:43:32.69 Exhaust.select :close @13:43:32.88 SSV.dial CBV @13:43:32.93
CBV.dial SSV @13:43:33.15 Session Begun @13:43:33.92
Allowing 130s for 25.0ml of remaining 875.0ml @13:43:33.94 Intake.select :open @13:43:36.17 Intake @30.3psia, vacuum from 20.0 to 24.0psi @13:43:36.19 SS.reconfigure SSinhale.with maxSpeed: 48 @13:43:36.30 SS.seek 25ml @13:44:13.28 Stroke completed -- waiting for pressure to equalize ... @13:44:14.96 Intake.select :close @13:44:15.14 SSV.dial :exhaust @13:44:16.05 Exhaust.select :open @13:44:16.20 SS.reconfigure SSexhale @13:44:16.27 SS.seek 0ml @13:44:25.76 Exhaust.select :close @13:44:25.91 SSV.dial CBV @13:44:25.93
CBV.dial SSV @13:44:26.86 Allowing 130s for 25.0ml of remaining 850.0ml @13:44:26.88 Intake.select :open @13:44:29.10 Intake @30.2psia, vacuum from 20.0 to 24.0psi @13:44:29.12 SS.reconfigure SSinhale.with maxSpeed: 48 @13:44:29.23 SS.seek 25ml @13:45:06.98 Stroke completed -- waiting for pressure to equalize ... @13:45:08.25 Intake.select :close @13:45:08.42 SSV.dial :exhaust @13:45:09.34 Exhaust.select :open @13:45:09.49 SS.reconfigure SSexhale @13:45:09.56 SS.seek 0ml @13:45:19.01 Exhaust.select :close @13:45:19.16 SSV.dial CBV @13:45:19.18
CBV.dial SSV @13:45:20.09 Allowing 130s for 25.0ml of remaining 825.0ml @13:45:20.11 Intake.select :open @13:45:22.36 Intake @30.2psia, vacuum from 20.0 to 24.0psi @13:45:22.38 SS.reconfigure SSinhale.with maxSpeed: 48 @13:45:22.49 SS.seek 25ml @13:46:00.01 Stroke completed -- waiting for pressure to equalize ... @13:46:01.28 Intake.select :close @13:46:01.46 SSV.dial :exhaust @13:46:02.37 Exhaust.select :open @13:46:02.52 SS.reconfigure SSexhale @13:46:02.59 SS.seek 0ml @13:46:12.03 Exhaust.select :close @13:46:12.18 SSV.dial CBV @13:46:12.20
CBV.dial SSV @13:46:13.12 Allowing 130s for 25.0ml of remaining 800.0ml @13:46:13.14 Intake.select :open @13:46:15.36 Intake @30.2psia, vacuum from 20.0 to 24.0psi @13:46:15.37 SS.reconfigure SSinhale.with maxSpeed: 48 @13:46:15.49 SS.seek 25ml @13:46:52.95 Stroke completed -- waiting for pressure to equalize ... @13:46:54.22 Intake.select :close @13:46:54.39 SSV.dial :exhaust @13:46:55.30 Exhaust.select :open @13:46:55.45 SS.reconfigure SSexhale @13:46:55.52 SS.seek 0ml @13:47:04.92 Exhaust.select :close @13:47:05.07 SSV.dial CBV @13:47:05.09
CBV.dial SSV @13:47:06.01 Allowing 130s for 25.0ml of remaining 775.0ml @13:47:06.04 Intake.select :open @13:47:08.24 Intake @30.3psia, vacuum from 20.0 to 24.0psi @13:47:08.26 SS.reconfigure SSinhale.with maxSpeed: 48 @13:47:08.37 SS.seek 25ml @13:47:45.33 Stroke completed -- waiting for pressure to equalize ... @13:47:46.60 Intake.select :close @13:47:46.77 SSV.dial :exhaust @13:47:47.69 Exhaust.select :open @13:47:47.84 SS.reconfigure SSexhale @13:47:47.91 SS.seek 0ml @13:47:57.19 Exhaust.select :close @13:47:57.34 SSV.dial CBV @13:47:57.36
CBV.dial SSV @13:47:58.28 Allowing 130s for 25.0ml of remaining 750.0ml @13:47:58.32 Intake.select :open @13:48:00.52 Intake @30.3psia, vacuum from 20.0 to 24.0psi @13:48:00.54 SS.reconfigure SSinhale.with maxSpeed: 48 @13:48:00.65 SS.seek 25ml @13:48:37.70 Stroke completed -- waiting for pressure to equalize ... @13:48:38.97 Intake.select :close @13:48:39.15 SSV.dial :exhaust @13:48:40.06 Exhaust.select :open @13:48:40.21 SS.reconfigure SSexhale @13:48:40.28 SS.seek 0ml @13:48:50.06 Exhaust.select :close @13:48:50.21 SSV.dial CBV @13:48:50.23
CBV.dial SSV @13:48:51.17 Allowing 130s for 25.0ml of remaining 725.0ml @13:48:51.19 Intake.select :open @13:48:53.41 Intake @30.2psia, vacuum from 20.0 to 24.0psi @13:48:53.43 SS.reconfigure SSinhale.with maxSpeed: 48 @13:48:53.54 SS.seek 25ml @13:49:30.54 Stroke completed -- waiting for pressure to equalize ... @13:49:32.42 Intake.select :close @13:49:32.59 SSV.dial :exhaust @13:49:33.50 Exhaust.select :open @13:49:33.65 SS.reconfigure SSexhale @13:49:33.72 SS.seek 0ml @13:49:42.69 Exhaust.select :close @13:49:42.85 SSV.dial CBV @13:49:42.87
CBV.dial SSV @13:49:43.78 Allowing 130s for 25.0ml of remaining 700.0ml @13:49:43.80 Intake.select :open @13:49:46.02 Intake @30.2psia, vacuum from 20.0 to 24.0psi @13:49:46.04 SS.reconfigure SSinhale.with maxSpeed: 48 @13:49:46.15 SS.seek 25ml @13:50:23.36 Stroke completed -- waiting for pressure to equalize ... @13:50:25.24 Intake.select :close @13:50:25.42 SSV.dial :exhaust @13:50:26.31 Exhaust.select :open @13:50:26.45 SS.reconfigure SSexhale @13:50:26.52 SS.seek 0ml @13:50:35.83 Exhaust.select :close @13:50:35.98 SSV.dial CBV @13:50:36.00
CBV.dial SSV @13:50:36.92 Allowing 130s for 25.0ml of remaining 675.0ml @13:50:36.94 Intake.select :open @13:50:39.16 Intake @30.1psia, vacuum from 20.0 to 24.0psi @13:50:39.19 SS.reconfigure SSinhale.with maxSpeed: 48 @13:50:39.30 SS.seek 25ml @13:51:16.33 Stroke completed -- waiting for pressure to equalize ... @13:51:18.20 Intake.select :close @13:51:18.37 SSV.dial :exhaust @13:51:19.28 Exhaust.select :open @13:51:19.43 SS.reconfigure SSexhale @13:51:19.50 SS.seek 0ml @13:51:28.91 Exhaust.select :close @13:51:29.03 SSV.dial CBV @13:51:29.05
CBV.dial SSV @13:51:29.98 Allowing 130s for 25.0ml of remaining 650.0ml @13:51:30.00 Intake.select :open @13:51:32.22 Intake @30.2psia, vacuum from 20.0 to 24.0psi @13:51:32.23 SS.reconfigure SSinhale.with maxSpeed: 48 @13:51:32.35 SS.seek 25ml @13:52:09.84 Stroke completed -- waiting for pressure to equalize ... @13:52:11.71 Intake.select :close @13:52:11.89 SSV.dial :exhaust @13:52:12.79 Exhaust.select :open @13:52:12.95 SS.reconfigure SSexhale @13:52:13.02 SS.seek 0ml @13:52:22.03 Exhaust.select :close @13:52:22.15 SSV.dial CBV @13:52:22.17
CBV.dial SSV @13:52:23.09 Allowing 130s for 25.0ml of remaining 625.0ml @13:52:23.11 Intake.select :open @13:52:25.33 Intake @30.1psia, vacuum from 20.0 to 24.0psi @13:52:25.35 SS.reconfigure SSinhale.with maxSpeed: 48 @13:52:25.46 SS.seek 25ml @13:52:45.06 Vacuum too high -- waiting up to 40s @13:52:45.59 Intake @27.4psia, vacuum from 20.0 to 24.0psi @13:52:45.61 SS.reconfigure SSinhale.with maxSpeed: 43 @13:52:45.71 SS.seek 25ml @13:53:06.20 Stroke completed -- waiting for pressure to equalize ... @13:53:07.90 Intake.select :close @13:53:08.08 SSV.dial :exhaust @13:53:08.98 Exhaust.select :open @13:53:09.13 SS.reconfigure SSexhale @13:53:09.21 SS.seek 0ml @13:53:18.14 Exhaust.select :close @13:53:18.29 SSV.dial CBV @13:53:18.31
CBV.dial SSV @13:53:19.23 Allowing 130s for 25.0ml of remaining 600.0ml @13:53:19.25 Intake.select :open @13:53:21.47 Intake @30.2psia, vacuum from 20.0 to 24.0psi @13:53:21.49 SS.reconfigure SSinhale.with maxSpeed: 43 @13:53:21.60 SS.seek 25ml @13:54:02.95 Stroke completed -- waiting for pressure to equalize ... @13:54:04.22 Intake.select :close @13:54:04.40 SSV.dial :exhaust @13:54:05.33 Exhaust.select :open @13:54:05.48 SS.reconfigure SSexhale @13:54:05.56 SS.seek 0ml @13:54:14.73 Exhaust.select :close @13:54:14.88 SSV.dial CBV @13:54:14.90
CBV.dial SSV @13:54:15.83 Allowing 130s for 25.0ml of remaining 575.0ml @13:54:15.85 Intake.select :open @13:54:18.07 Intake @30.1psia, vacuum from 20.0 to 24.0psi @13:54:18.09 SS.reconfigure SSinhale.with maxSpeed: 43 @13:54:18.20 SS.seek 25ml @13:54:59.34 Stroke completed -- waiting for pressure to equalize ... @13:55:00.61 Intake.select :close @13:55:00.79 SSV.dial :exhaust @13:55:01.70 Exhaust.select :open @13:55:01.85 SS.reconfigure SSexhale @13:55:01.92 SS.seek 0ml @13:55:11.11 Exhaust.select :close @13:55:11.26 SSV.dial CBV @13:55:11.28
CBV.dial SSV @13:55:12.20 Allowing 130s for 25.0ml of remaining 550.0ml @13:55:12.23 Intake.select :open @13:55:14.44 Intake @29.9psia, vacuum from 20.0 to 24.0psi @13:55:14.46 SS.reconfigure SSinhale.with maxSpeed: 43 @13:55:14.57 SS.seek 25ml @13:55:55.78 Stroke completed -- waiting for pressure to equalize ... @13:55:57.05 Intake.select :close @13:55:57.22 SSV.dial :exhaust @13:55:58.14 Exhaust.select :open @13:55:58.29 SS.reconfigure SSexhale @13:55:58.37 SS.seek 0ml @13:56:07.79 Exhaust.select :close @13:56:07.94 SSV.dial CBV @13:56:07.96
CBV.dial SSV @13:56:08.87 Allowing 130s for 25.0ml of remaining 525.0ml @13:56:08.89 Intake.select :open @13:56:11.11 Intake @30.0psia, vacuum from 20.0 to 24.0psi @13:56:11.13 SS.reconfigure SSinhale.with maxSpeed: 43 @13:56:11.24 SS.seek 25ml @13:56:52.84 Stroke completed -- waiting for pressure to equalize ... @13:56:54.11 Intake.select :close @13:56:54.28 SSV.dial :exhaust @13:56:55.20 Exhaust.select :open @13:56:55.35 SS.reconfigure SSexhale @13:56:55.42 SS.seek 0ml @13:57:04.26 Exhaust.select :close @13:57:04.42 SSV.dial CBV @13:57:04.44
CBV.dial SSV @13:57:05.36 Allowing 130s for 25.0ml of remaining 500.0ml @13:57:05.38 Intake.select :open @13:57:07.60 Intake @29.9psia, vacuum from 20.0 to 24.0psi @13:57:07.62 SS.reconfigure SSinhale.with maxSpeed: 43 @13:57:07.74 SS.seek 25ml @13:57:49.67 Stroke completed -- waiting for pressure to equalize ... @13:57:50.94 Intake.select :close @13:57:51.12 SSV.dial :exhaust @13:57:52.03 Exhaust.select :open @13:57:52.61 SS.reconfigure SSexhale @13:57:52.69 SS.seek 0ml @13:58:02.01 Exhaust.select :close @13:58:02.16 SSV.dial CBV @13:58:02.18
CBV.dial SSV @13:58:03.11 Allowing 130s for 25.0ml of remaining 475.0ml @13:58:03.13 Intake.select :open @13:58:05.35 Intake @29.8psia, vacuum from 20.0 to 24.0psi @13:58:05.37 SS.reconfigure SSinhale.with maxSpeed: 43 @13:58:05.48 SS.seek 25ml @13:58:46.57 Stroke completed -- waiting for pressure to equalize ... @13:58:48.45 Intake.select :close @13:58:48.63 SSV.dial :exhaust @13:58:49.53 Exhaust.select :open @13:58:49.69 SS.reconfigure SSexhale @13:58:49.76 SS.seek 0ml @13:58:59.04 Exhaust.select :close @13:58:59.19 SSV.dial CBV @13:58:59.21
CBV.dial SSV @13:59:00.12 Allowing 130s for 25.0ml of remaining 450.0ml @13:59:00.14 Intake.select :open @13:59:02.36 Intake @29.9psia, vacuum from 20.0 to 24.0psi @13:59:02.38 SS.reconfigure SSinhale.with maxSpeed: 43 @13:59:02.49 SS.seek 25ml @13:59:43.84 Stroke completed -- waiting for pressure to equalize ... @13:59:45.72 Intake.select :close @13:59:45.91 SSV.dial :exhaust @13:59:46.83 Exhaust.select :open @13:59:46.98 SS.reconfigure SSexhale @13:59:47.05 SS.seek 0ml @13:59:56.29 Exhaust.select :close @13:59:56.44 SSV.dial CBV @13:59:56.46
CBV.dial SSV @13:59:57.37 Allowing 130s for 25.0ml of remaining 425.0ml @13:59:57.39 Intake.select :open @13:59:59.61 Intake @29.8psia, vacuum from 20.0 to 24.0psi @13:59:59.63 SS.reconfigure SSinhale.with maxSpeed: 43 @13:59:59.74 SS.seek 25ml @14:00:40.89 Stroke completed -- waiting for pressure to equalize ... @14:00:42.76 Intake.select :close @14:00:42.95 SSV.dial :exhaust @14:00:43.86 Exhaust.select :open @14:00:44.02 SS.reconfigure SSexhale @14:00:44.09 SS.seek 0ml @14:00:53.53 Exhaust.select :close @14:00:53.68 SSV.dial CBV @14:00:53.70
CBV.dial SSV @14:00:54.62 Allowing 130s for 25.0ml of remaining 400.0ml @14:00:54.65 Intake.select :open @14:00:56.86 Intake @29.7psia, vacuum from 20.0 to 24.0psi @14:00:56.88 SS.reconfigure SSinhale.with maxSpeed: 43 @14:00:56.99 SS.seek 25ml @14:01:38.59 Stroke completed -- waiting for pressure to equalize ... @14:01:40.48 Intake.select :close @14:01:40.66 SSV.dial :exhaust @14:01:41.56 Exhaust.select :open @14:01:41.70 SS.reconfigure SSexhale @14:01:41.78 SS.seek 0ml @14:01:51.06 Exhaust.select :close @14:01:51.21 SSV.dial CBV @14:01:51.23
CBV.dial SSV @14:01:52.15 Allowing 130s for 25.0ml of remaining 375.0ml @14:01:52.18 Intake.select :open @14:01:54.39 Intake @29.7psia, vacuum from 20.0 to 24.0psi @14:01:54.41 SS.reconfigure SSinhale.with maxSpeed: 43 @14:01:54.52 SS.seek 25ml @14:02:35.82 Stroke completed -- waiting for pressure to equalize ... @14:02:37.68 Intake.select :close @14:02:38.20 SSV.dial :exhaust @14:02:39.18 Exhaust.select :open @14:02:39.33 SS.reconfigure SSexhale @14:02:39.41 SS.seek 0ml @14:02:48.82 Exhaust.select :close @14:02:48.97 SSV.dial CBV @14:02:48.99
CBV.dial SSV @14:02:49.92 Allowing 130s for 25.0ml of remaining 350.0ml @14:02:49.94 Intake.select :open @14:02:52.16 Intake @29.7psia, vacuum from 20.0 to 24.0psi @14:02:52.22 SS.reconfigure SSinhale.with maxSpeed: 43 @14:02:52.29 SS.seek 25ml @14:03:33.90 Stroke completed -- waiting for pressure to equalize ... @14:03:35.78 Intake.select :close @14:03:35.94 SSV.dial :exhaust @14:03:36.84 Exhaust.select :open @14:03:37.00 SS.reconfigure SSexhale @14:03:37.07 SS.seek 0ml @14:03:46.31 Exhaust.select :close @14:03:46.46 SSV.dial CBV @14:03:46.48
CBV.dial SSV @14:03:47.42 Allowing 130s for 25.0ml of remaining 325.0ml @14:03:47.45 Intake.select :open @14:03:49.65 Intake @29.7psia, vacuum from 20.0 to 24.0psi @14:03:49.66 SS.reconfigure SSinhale.with maxSpeed: 43 @14:03:49.78 SS.seek 25ml @14:04:31.09 Stroke completed -- waiting for pressure to equalize ... @14:04:32.97 Intake.select :close @14:04:33.15 SSV.dial :exhaust @14:04:34.06 Exhaust.select :open @14:04:34.21 SS.reconfigure SSexhale @14:04:34.28 SS.seek 0ml @14:04:43.68 Exhaust.select :close @14:04:43.84 SSV.dial CBV @14:04:43.86
CBV.dial SSV @14:04:44.78 Allowing 130s for 25.0ml of remaining 300.0ml @14:04:44.80 Intake.select :open @14:04:47.02 Intake @29.6psia, vacuum from 20.0 to 24.0psi @14:04:47.04 SS.reconfigure SSinhale.with maxSpeed: 43 @14:04:47.15 SS.seek 25ml @14:05:28.78 Stroke completed -- waiting for pressure to equalize ... @14:05:30.65 Intake.select :close @14:05:30.83 SSV.dial :exhaust @14:05:31.73 Exhaust.select :open @14:05:31.88 SS.reconfigure SSexhale @14:05:31.95 SS.seek 0ml @14:05:41.20 Exhaust.select :close @14:05:41.35 SSV.dial CBV @14:05:41.37
CBV.dial SSV @14:05:42.29 Allowing 130s for 25.0ml of remaining 275.0ml @14:05:42.31 Intake.select :open @14:05:44.53 Intake @29.7psia, vacuum from 20.0 to 24.0psi @14:05:44.55 SS.reconfigure SSinhale.with maxSpeed: 43 @14:05:44.67 SS.seek 25ml @14:05:55.62 Vacuum too high -- waiting up to 40s @14:05:56.15 Intake @27.6psia, vacuum from 20.0 to 24.0psi @14:05:56.17 SS.reconfigure SSinhale.with maxSpeed: 39 @14:05:56.28 SS.seek 25ml @14:06:29.92 Stroke completed -- waiting for pressure to equalize ... @14:06:31.79 Intake.select :close @14:06:31.94 SSV.dial :exhaust @14:06:32.86 Exhaust.select :open @14:06:33.01 SS.reconfigure SSexhale @14:06:33.08 SS.seek 0ml @14:06:42.46 Exhaust.select :close @14:06:42.61 SSV.dial CBV @14:06:42.63
CBV.dial SSV @14:06:43.54 Allowing 130s for 25.0ml of remaining 250.0ml @14:06:43.57 Intake.select :open @14:06:45.79 Intake @29.7psia, vacuum from 20.0 to 24.0psi @14:06:46.16 SS.reconfigure SSinhale.with maxSpeed: 39 @14:06:46.34 SS.seek 25ml @14:07:31.93 Stroke completed -- waiting for pressure to equalize ... @14:07:33.81 Intake.select :close @14:07:34.00 SSV.dial :exhaust @14:07:34.90 Exhaust.select :open @14:07:35.05 SS.reconfigure SSexhale @14:07:35.12 SS.seek 0ml @14:07:44.35 Exhaust.select :close @14:07:44.50 SSV.dial CBV @14:07:44.52
CBV.dial SSV @14:07:45.43 Allowing 130s for 25.0ml of remaining 225.0ml @14:07:45.45 Intake.select :open @14:07:47.67 Intake @29.6psia, vacuum from 20.0 to 24.0psi @14:07:47.69 SS.reconfigure SSinhale.with maxSpeed: 39 @14:07:47.81 SS.seek 25ml @14:08:33.00 Stroke completed -- waiting for pressure to equalize ... @14:08:34.88 Intake.select :close @14:08:35.06 SSV.dial :exhaust @14:08:35.96 Exhaust.select :open @14:08:36.11 SS.reconfigure SSexhale @14:08:36.18 SS.seek 0ml @14:08:45.10 Exhaust.select :close @14:08:45.25 SSV.dial CBV @14:08:45.27
CBV.dial SSV @14:08:46.18 Allowing 130s for 25.0ml of remaining 200.0ml @14:08:46.20 Intake.select :open @14:08:48.42 Intake @29.7psia, vacuum from 20.0 to 24.0psi @14:08:48.44 SS.reconfigure SSinhale.with maxSpeed: 39 @14:08:48.55 SS.seek 25ml @14:09:34.56 Stroke completed -- waiting for pressure to equalize ... @14:09:36.43 Intake.select :close @14:09:36.59 SSV.dial :exhaust @14:09:37.51 Exhaust.select :open @14:09:37.66 SS.reconfigure SSexhale @14:09:37.73 SS.seek 0ml @14:09:47.06 Exhaust.select :close @14:09:47.21 SSV.dial CBV @14:09:47.23
CBV.dial SSV @14:09:48.15 Allowing 130s for 25.0ml of remaining 175.0ml @14:09:48.17 Intake.select :open @14:09:50.39 Intake @29.6psia, vacuum from 20.0 to 24.0psi @14:09:50.41 SS.reconfigure SSinhale.with maxSpeed: 39 @14:09:50.52 SS.seek 25ml @14:10:35.70 Stroke completed -- waiting for pressure to equalize ... @14:10:37.58 Intake.select :close @14:10:37.76 SSV.dial :exhaust @14:10:38.67 Exhaust.select :open @14:10:38.82 SS.reconfigure SSexhale @14:10:38.89 SS.seek 0ml @14:10:48.14 Exhaust.select :close @14:10:48.29 SSV.dial CBV @14:10:48.31
CBV.dial SSV @14:10:49.25 Allowing 130s for 25.0ml of remaining 150.0ml @14:10:49.27 Intake.select :open @14:10:51.49 Intake @29.7psia, vacuum from 20.0 to 24.0psi @14:10:51.51 SS.reconfigure SSinhale.with maxSpeed: 39 @14:10:51.62 SS.seek 25ml @14:11:37.12 Stroke completed -- waiting for pressure to equalize ... @14:11:39.00 Intake.select :close @14:11:39.18 SSV.dial :exhaust @14:11:40.09 Exhaust.select :open @14:11:40.24 SS.reconfigure SSexhale @14:11:40.31 SS.seek 0ml @14:11:49.51 Exhaust.select :close @14:11:49.66 SSV.dial CBV @14:11:49.68
CBV.dial SSV @14:11:50.95 Allowing 130s for 25.0ml of remaining 125.0ml @14:11:51.04 Intake.select :open @14:11:53.26 Intake @29.7psia, vacuum from 20.0 to 24.0psi @14:11:53.28 SS.reconfigure SSinhale.with maxSpeed: 39 @14:11:53.39 SS.seek 25ml @14:12:38.70 Stroke completed -- waiting for pressure to equalize ... @14:12:41.19 Intake.select :close @14:12:41.36 SSV.dial :exhaust @14:12:42.28 Exhaust.select :open @14:12:42.43 SS.reconfigure SSexhale @14:12:42.50 SS.seek 0ml @14:12:51.93 Exhaust.select :close @14:12:52.08 SSV.dial CBV @14:12:52.10
CBV.dial SSV @14:12:53.03 Allowing 130s for 25.0ml of remaining 100.0ml @14:12:53.05 Intake.select :open @14:12:55.27 Intake @29.7psia, vacuum from 20.0 to 24.0psi @14:12:55.29 SS.reconfigure SSinhale.with maxSpeed: 39 @14:12:55.40 SS.seek 25ml @14:13:41.03 Stroke completed -- waiting for pressure to equalize ... @14:13:42.91 Intake.select :close @14:13:43.07 SSV.dial :exhaust @14:13:43.98 Exhaust.select :open @14:13:44.14 SS.reconfigure SSexhale @14:13:44.21 SS.seek 0ml @14:13:53.12 Exhaust.select :close @14:13:53.27 SSV.dial CBV @14:13:53.29
CBV.dial SSV @14:13:54.21 Allowing 130s for 25.0ml of remaining 75.0ml @14:13:54.23 Intake.select :open @14:13:56.45 Intake @29.5psia, vacuum from 20.0 to 24.0psi @14:13:56.47 SS.reconfigure SSinhale.with maxSpeed: 39 @14:13:56.58 SS.seek 25ml @14:14:25.15 Session Ended @14:14:42.48
Stroke completed -- waiting for pressure to equalize ... @14:14:44.36 Intake.select :close @14:14:44.52 SSV.dial :exhaust @14:14:45.42 Exhaust.select :open @14:14:45.56 SS.reconfigure SSexhale @14:14:45.63 SS.seek 0ml @14:14:53.04 Session Begun @14:14:54.82
Exhaust.select :close @14:14:54.98 SSV.dial CBV @14:14:55.00
CBV.dial SSV @14:14:55.92 Allowing 130s for 25.0ml of remaining 50.0ml @14:14:55.94 Intake.select :open @14:14:58.21 Intake @29.6psia, vacuum from 20.0 to 24.0psi @14:14:58.23 SS.reconfigure SSinhale.with maxSpeed: 39 @14:14:58.34 SS.seek 25ml @14:15:43.57 Stroke completed -- waiting for pressure to equalize ... @14:15:45.44 Intake.select :close @14:15:45.62 SSV.dial :exhaust @14:15:46.53 Exhaust.select :open @14:15:46.68 SS.reconfigure SSexhale @14:15:46.75 SS.seek 0ml @14:15:56.15 Exhaust.select :close @14:15:56.30 SSV.dial CBV @14:15:56.32
CBV.dial SSV @14:15:57.24 Allowing 130s for 25.0ml of remaining 25.0ml @14:15:57.26 Intake.select :open @14:15:59.48 Intake @29.6psia, vacuum from 20.0 to 24.0psi @14:15:59.50 SS.reconfigure SSinhale.with maxSpeed: 39 @14:15:59.61 SS.seek 24.97ml @14:16:11.93 Vacuum too high -- waiting up to 40s @14:16:12.23 Intake @27.1psia, vacuum from 20.0 to 24.0psi @14:16:12.25 SS.reconfigure SSinhale.with maxSpeed: 35 @14:16:12.35 SS.seek 24.97ml @14:16:49.31 Stroke completed -- waiting for pressure to equalize ... @14:16:51.61 Intake.select :close @14:16:51.79 SSV.dial :exhaust @14:16:52.70 Exhaust.select :open @14:16:52.87 SS.reconfigure SSexhale @14:16:52.95 SS.seek 0ml @14:17:02.23 Exhaust.select :close @14:17:02.38 Sampled 1500.0ml @14:17:02.40 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @14:17:02.42 Intake.select :close @14:17:02.55 CSV.dial :waste @14:17:02.65 CS.seek 0ml @14:17:02.76 SSV.dial CBV,avoiding: :exhaust @14:17:02.79 CSV.dial :air @14:17:02.89
CBV.dial SSV @14:17:03.91 CS.seek 10ml @14:17:05.03
CTV.dial :air @14:17:05.38 Gate.power :sampler,:OFF @14:17:05.94
delay 15 seconds @14:17:20.96 SSV.dial :spit,avoiding: :exhaust @14:17:22.51 CTV.dial CSV,avoiding: :intake @14:17:22.60
SS.seek 0ml @14:17:22.74 SSV.dial CBV,avoiding: :exhaust @14:17:31.28 CBV.dial between SSV and waste2 @14:17:31.72 CTV.dial CSV,avoiding: :air @14:17:31.75
CSV.dial CTV @14:17:32.34 CS.reconfigure CSpressurize @14:17:32.43 CS.seek 0ml @14:17:56.88 BubblePoint: Intake @67.6psia, Outlet @11.9psia @14:17:56.89 CBV.dial SSV @14:17:57.32 SS.reconfigure SSconfig @14:17:57.41 SS.seek 10ml @14:18:03.21 delay 15 seconds @14:18:18.34 Evacuated: Intake @30.4psia, Outlet @-2.2psia @14:18:18.35 SSV.dial :spit,avoiding: :exhaust @14:18:19.93 SS.reconfigure SSexhale @14:18:20.02 SS.seek 0ml @14:18:25.16 SS.reconfigure SSfast @14:18:25.24 SS.seek 5ml @14:18:28.20 SSV.dial CBV,avoiding: :exhaust @14:18:28.23
CBV.dial SSV @14:18:29.80 CTV.dial :air,avoiding: :intake @14:18:31.38 CSV.dial CTV @14:18:31.45
SS.seek 10ml @14:18:32.41 CSV.dial :air @14:18:32.96 CS.reconfigure CSfast @14:18:33.04 CS.seek 0ml @14:18:34.21
SSV.dial :spit,avoiding: :exhaust @14:18:35.78 SS.reconfigure SSexhale @14:18:35.86 SS.seek 0ml @14:18:35.92 CS.coast @14:18:41.38
SS.reconfigure SSfast @14:18:41.46 SS.seek 5ml @14:18:44.15 SSV.dial CBV,avoiding: :exhaust @14:18:44.23
CBV.dial SSV @14:18:46.72 SSV.dial :spit,avoiding: :exhaust @14:18:48.30 SS.reconfigure SSconfig @14:18:48.38 SS.seek 0ml @14:18:51.90 SS.coast @14:18:51.99 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @14:18:52.00 Intake.select :close @14:18:52.12 CSV.dial :waste @14:18:52.26 SSV.dial CBV,avoiding: :exhaust @14:18:52.29
CBV.dial SSV @14:18:53.05 CS.seek 0ml @14:18:53.16 CSV.dial :air @14:18:53.85
CTV.dial :air @14:18:53.87 delay 15 seconds @14:18:54.47 CS.seek 10ml @14:19:08.91
SSV.dial :spit,avoiding: :exhaust @14:19:10.47 CTV.dial CSV,avoiding: :intake @14:19:10.51
SS.reconfigure SSexhale @14:19:10.67 SS.seek 0ml @14:19:10.85 SSV.dial CBV,avoiding: :exhaust @14:19:21.47 CBV.dial between SSV and waste2 @14:19:21.93 CTV.dial CSV,avoiding: :air @14:19:21.96
CSV.dial CTV @14:19:22.53 CS.reconfigure CSpressurize @14:19:22.61 CS.seek 0ml @14:19:48.82 CSV.dial :air @14:19:49.38 CS.reconfigure CSfast @14:19:49.47 CS.seek 10ml @14:20:16.04 CSV.dial between air and CTV @14:20:16.44 CS.seek 3.172ml @14:20:35.01 CTV.dial CSV,avoiding: :air @14:20:35.04
CSV.dial CTV @14:20:35.44 CS.reconfigure CSpressurize @14:20:35.52 CS.seek 0ml @14:20:43.19 BubblePoint: Intake @66.8psia, Outlet @11.8psia @14:20:43.20 CBV.dial SSV @14:20:43.64 SS.reconfigure SSconfig @14:20:43.72 SS.seek 10ml @14:20:49.09 delay 15 seconds @14:21:04.22 Evacuated: Intake @36.0psia, Outlet @14.1psia @14:21:04.23 SSV.dial :spit,avoiding: :exhaust @14:21:05.82 SS.reconfigure SSexhale @14:21:05.90 SS.seek 0ml @14:21:11.23 SS.reconfigure SSfast @14:21:11.31 SS.seek 5ml @14:21:14.35 SSV.dial CBV,avoiding: :exhaust @14:21:14.38
CBV.dial SSV @14:21:15.94 CTV.dial :air,avoiding: :intake @14:21:17.52 CSV.dial CTV @14:21:17.59
SS.seek 10ml @14:21:18.55 CSV.dial :air @14:21:19.10 CS.reconfigure CSfast @14:21:19.18 CS.seek 0ml @14:21:20.54
SSV.dial :spit,avoiding: :exhaust @14:21:21.60 CS.coast @14:21:22.07
SS.reconfigure SSexhale @14:21:22.16 SS.seek 0ml @14:21:27.58 SS.reconfigure SSfast @14:21:27.66 SS.seek 5ml @14:21:30.53 SSV.dial CBV,avoiding: :exhaust @14:21:30.56
CBV.dial SSV @14:21:33.15 SSV.dial :spit,avoiding: :exhaust @14:21:34.71 SS.reconfigure SSconfig @14:21:34.80 SS.seek 0ml @14:21:37.93 SS.coast @14:21:38.00 Lysing: Adding 1.5ml lysis @14:21:38.01 CBV.dial between CSV and waste1,avoiding: :DBV @14:21:39.79 CTV.dial CSV @14:21:41.35 CSV.dial CSR @14:21:42.29 Pulling 1ml of CSR.lysis CSR.select :lysis @14:21:42.91 CS.reconfigure CSconfig @14:21:43.01 CS.seek 1ml @14:21:46.63 delay 5 seconds @14:21:51.65 CSR.select :air @14:21:51.85 CS.seek 6ml @14:22:05.70 delay 2 seconds @14:22:07.72 CSV.dial :waste1 @14:22:09.24 CS.reconfigure CSfast @14:22:09.34 CS.seek :empty @14:22:25.51 CSV.dial CSR @14:22:27.11 Pulling 1.5ml of CSR.lysis @14:22:27.12 CSR.select :lysis @14:22:27.29 CS.reconfigure CSconfig @14:22:27.36 CS.seek 1.5ml @14:22:32.23 delay 5 seconds @14:22:37.25 CSR.select :air @14:22:37.45 CS.seek 4.5ml @14:22:46.20 delay 2 seconds @14:22:48.22 CSV.dial CTV @14:22:48.77 CS.seek 1.275ml @14:22:58.14 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @14:22:58.16 CH.seek 85C,8:00 @14:26:02.81 CH.hold 5:00,85C @14:31:02.99 Collection Puck Heater at 85C after lysing @14:31:03.01 CS.seek 0ml @14:31:07.36 CTV.dial between spare and air,avoiding: :intake @14:31:08.59 CSV.dial :air @14:31:09.13 CS.seek 5ml @14:31:22.89 CSV.dial CTV @14:31:23.45 delay 2 seconds @14:31:25.47 CSV.dial :air @14:31:26.03 delay 2 seconds @14:31:28.05 CSV.dial CTV @14:31:28.72 delay 2 seconds @14:31:30.74 CSV.dial :air @14:31:31.75 delay 2 seconds @14:31:33.78 CS.seek 0ml @14:31:47.64 Recovering Lysate into Collection syringe @14:31:47.65 CSV.dial CBV @14:31:48.88 CBV.dial CSV @14:31:49.34 CS.seek 5ml @14:32:03.17 delay 5 seconds @14:32:08.20 CTV.dial :air @14:32:08.83 CS.seek 7ml @14:32:15.02 delay 5 seconds @14:32:20.05 CSV.dial :air2 @14:32:20.59 CS.seek 10ml @14:32:29.24 CSV.dial between air and CTV,avoiding: CBV @14:32:30.59 Lysing Complete @14:32:30.70 Storing sh1 puck in Collection Clamp to tube 1 @14:32:30.90 SC.reconfigure SCtight @14:32:30.99 SC.seek 1 @14:32:33.13 SC.reconfigure SCloose @14:32:33.37 Elbow.reconfigure TightElbow @14:32:33.46 Elbow.seek 1 @14:32:35.42 Forearm.reconfigure TightForearm @14:32:35.51 Forearm.seek 1 + 700 counts @14:32:36.58 SE.reconfigure SEpick @14:32:36.69 SE.seek beamBreak @14:32:39.71 SE.configure SEconfig @14:32:39.80 SE.seek :place @14:32:40.36 Forearm.reconfigure LooseForearm @14:32:40.44 Forearm.seek :retract @14:32:41.38 Elbow.seek CC @14:32:43.00 Elbow.reconfigure LooseElbow @14:32:43.13 Forearm.seek CC @14:32:44.15 Hand.close @14:32:44.96 CC.seek :open @14:32:49.49 Forearm.seek :retract @14:32:50.52 Elbow.reconfigure TightElbow @14:32:50.60 Elbow.seek 1 @14:32:52.51 Elbow.reconfigure LimpElbow @14:32:52.60 Forearm.seek 1 @14:32:53.64 Elbow.hold @14:32:53.75 Elbow.reconfigure TightElbow @14:32:53.82 Hand.open @14:32:54.69 Forearm.force -20 @14:32:54.78 Forearm.seek 1 + 344 counts @14:32:55.09 Forearm.reconfigure TightForearm @14:32:55.17 Forearm.seek 1 + 700 counts @14:32:55.58 SE.seek :down @14:32:57.16
Forearm.reconfigure LooseForearm @14:32:57.25 Forearm.seek :retract @14:32:58.38 SE.coast @14:32:58.46
SC.coast @14:32:58.53 Tranferring lysate to PRV @14:32:58.54 CSV.dial :air @14:32:58.93 CS.seek 10ml @14:32:59.05 CSV.dial between air and CTV @14:32:59.42 AIV.dial :bypass @14:32:59.54 Beginning samplePassC @14:32:59.55 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:32:59.69 Hand.open @14:33:00.08 Elbow.seek FlushPuck::Garage @14:33:02.07 Elbow.reconfigure LooseElbow @14:33:02.20 Forearm.seek :garage @14:33:03.26 Hand.close @14:33:04.19 Forearm.seek :retract @14:33:05.20 Elbow.reconfigure TightElbow @14:33:05.29 Elbow.seek CC @14:33:08.19 Elbow.reconfigure LooseElbow @14:33:08.32 Forearm.reconfigure TightForearm @14:33:08.39 Forearm.seek CC @14:33:09.67 CC.seek :closed @14:33:13.95 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.25 @14:33:14.06 Hand.open @14:33:14.89 Forearm.reconfigure LooseForearm @14:33:14.97 Forearm.seek :retract @14:33:15.99 AWV.select :air @14:33:16.00 AIV.dial :bypass @14:33:16.05 PSV.dial PSR @14:33:17.63 Pulling 1.5ml of PSR.flush @14:33:17.64 PSR.select :flush @14:33:17.83 PS.seek 1.5ml @14:33:34.24 delay 5 seconds @14:33:39.26 PSV.dial :waste1 @14:33:39.84 PS.seek 0ml @14:33:56.36 PSV.dial PSR @14:33:56.92 PSR.select :air @14:33:57.07 PS.seek 2.5ml @14:34:23.89 PRV.dial CBV @14:34:23.92
CBV.dial PRV @14:34:24.86 PSV.dial :mixloop @14:34:26.05 CTV.dial :air,avoiding: :intake @14:34:26.06 PTV.dial :PRVmixing @14:34:26.22 PS.seek 0ml @14:34:53.17 PSV.dial :air @14:34:53.72 PS.seek 2.5ml @14:35:20.46 PSV.dial :mixloop @14:35:21.01 PS.seek 0ml @14:35:47.71 PSV.dial :air @14:35:48.26 PS.seek 2.5ml @14:36:14.93 PSV.dial :mixloop @14:36:15.93 PS.seek 0ml @14:36:42.68 Garaging FlushPuck in Collection Clamp @14:36:42.80 Elbow.reconfigure TightElbow @14:36:42.87 Elbow.seek CC @14:36:43.15 Elbow.reconfigure LooseElbow @14:36:43.28 Forearm.seek CC @14:36:44.31 Hand.close @14:36:45.09 CC.seek :open @14:36:49.51 Forearm.seek :retract @14:36:50.56 Elbow.reconfigure TightElbow @14:36:50.64 Elbow.seek :garage @14:36:53.79 Elbow.reconfigure LooseElbow @14:36:53.92 Forearm.reconfigure TightForearm @14:36:53.99 Forearm.seek :garage @14:36:55.21 Hand.open @14:36:56.02 Forearm.reconfigure LooseForearm @14:36:56.11 Forearm.seek :retract @14:36:57.18 Connecting PS to CS to transfer sample @14:36:57.19 CBV.dial AIV @14:36:57.24 AIV.dial :bypass @14:36:57.27 PSV.dial :mixloop @14:36:57.31 PTV.dial :PRVmixing @14:36:57.35 PRV.dial AIV @14:36:57.40 CTV.dial :air,avoiding: :intake @14:36:57.45 Loading lyfil puck from tube 2 into Collection Clamp @14:36:57.73 SC.reconfigure SCtight @14:36:57.81 SC.seek 2 @14:37:00.00 SC.reconfigure SCloose @14:37:00.25 Elbow.reconfigure TightElbow @14:37:00.33 Elbow.seek 2 @14:37:02.52 Forearm.reconfigure TightForearm @14:37:02.60 Forearm.seek 2 + 700 counts @14:37:03.66 SE.reconfigure SEpick @14:37:03.77 SE.seek beamBreak @14:37:06.91 SE.configure SEconfig @14:37:07.09 Elbow.seek 2 @14:37:07.31 Forearm.seek 2 + 300 counts @14:37:07.64 Forearm.reconfigure LooseForearm @14:37:07.72 Hand.close @14:37:08.47 SE.seek :down @14:37:08.69
Forearm.seek :retract @14:37:09.70 Elbow.seek CC @14:37:11.30 SE.coast @14:37:11.45
Elbow.reconfigure LooseElbow @14:37:11.57 Forearm.reconfigure TightForearm @14:37:11.65 Forearm.seek CC @14:37:12.92 CC.seek :closed @14:37:17.30 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.35 @14:37:17.31 Hand.open @14:37:18.60 Forearm.reconfigure LooseForearm @14:37:18.70 Forearm.seek :retract @14:37:19.73 SC.coast @14:37:19.80 CTV.dial CSV,avoiding: :intake @14:37:21.38 CSV.dial CTV @14:37:21.75 CS.seek 5ml @14:37:35.58 delay 1:20 @14:38:55.62 CS.seek 2.5ml @14:39:03.02 delay 40 seconds @14:39:43.06 CS.seek 0ml @14:39:50.49 delay 1:00 @14:40:50.52 PSV.dial :air @14:40:51.08 PS.seek 2.5ml @14:41:17.99 PSV.dial :mixloop @14:41:18.52 delay 3 seconds @14:41:21.54 PSV.dial :air @14:41:22.05 delay 2 seconds @14:41:24.07 PSV.dial :mixloop @14:41:24.58 delay 3 seconds @14:41:27.60 PSV.dial :waste1 @14:41:29.13 PS.seek :empty @14:41:56.02 CBV.dial :waste1 @14:41:56.57 delay 5 seconds @14:42:01.59 CBV.dial AIV @14:42:02.12 delay 10 seconds @14:42:12.15 CBV.dial :waste1 @14:42:12.71 delay 10 seconds @14:42:22.74 CBV.dial AIV @14:42:23.29 delay 10 seconds @14:42:33.32 CTV.dial CSV,avoiding: :intake @14:42:33.33 CBV.dial between AIV and waste1 @14:42:33.76 CSV.dial :air @14:42:34.32 CS.seek 10ml @14:43:00.96 CSV.dial CTV @14:43:01.54 CS.seek 0ml @14:43:28.21 CBV.dial :waste1 @14:43:28.57 delay 10 seconds @14:43:38.60 CTV.dial :air,avoiding: :intake @14:43:40.22 Storing lyfil puck in Collection Clamp to tube 1 @14:43:40.42 SC.reconfigure SCtight @14:43:40.50 SC.seek 1 @14:43:42.65 SC.reconfigure SCloose @14:43:42.88 Elbow.reconfigure TightElbow @14:43:42.96 Elbow.seek 1 @14:43:44.89 Forearm.reconfigure TightForearm @14:43:44.97 Forearm.seek 1 + 700 counts @14:43:46.00 SE.reconfigure SEpick @14:43:46.11 SE.seek beamBreak @14:43:49.31 SE.configure SEconfig @14:43:49.49 Elbow.reconfigure LimpElbow @14:43:49.57 Forearm.reconfigure LooseForearm @14:43:49.65 Forearm.seek 1 @14:43:49.99 Elbow.hold @14:43:50.09 Elbow.reconfigure TightElbow @14:43:50.22 SE.seek 0.03 Pucks below Top Plate @14:43:50.68 Forearm.force -20 @14:43:50.76 Forearm.seek 1 + 338 counts @14:43:51.07 Forearm.reconfigure TightForearm @14:43:51.15 Forearm.seek 1 + 700 counts @14:43:51.51 SE.seek 1.03 Pucks below Top Plate @14:43:52.13 Forearm.reconfigure LooseForearm @14:43:52.21 Forearm.seek :retract @14:43:53.17 Elbow.seek CC @14:43:54.94 Elbow.reconfigure LooseElbow @14:43:55.52 Forearm.seek CC @14:43:56.53 Hand.close @14:43:57.35 CC.seek :open @14:44:01.87 Forearm.seek :retract @14:44:02.86 Elbow.reconfigure TightElbow @14:44:02.94 Elbow.seek 1 @14:44:04.98 Elbow.reconfigure LimpElbow @14:44:05.07 Forearm.seek 1 @14:44:06.14 Elbow.hold @14:44:06.24 Elbow.reconfigure TightElbow @14:44:06.32 Hand.open @14:44:07.23 Forearm.force -20 @14:44:07.31 Forearm.seek 1 + 328 counts @14:44:07.65 Forearm.reconfigure TightForearm @14:44:07.74 Forearm.seek 1 + 700 counts @14:44:08.06 SE.seek :down @14:44:09.20
Forearm.reconfigure LooseForearm @14:44:09.29 Forearm.seek :retract @14:44:10.73 SE.coast @14:44:10.82
SC.coast @14:44:10.88 PSV.dial :air @14:44:12.25 PS.seek 0.499ml @14:44:18.24 delay 3 seconds @14:44:21.26 PSV.dial :mixloop @14:44:21.81 PRV.dial :air @14:44:22.37 delay 3 seconds @14:44:25.39 PRV.dial CBV @14:44:25.95 CBV.dial AIV @14:44:26.50 PRV.dial between CBV and air @14:44:26.90 PRV.dial :air @14:44:27.30 PS.reconfigure PSslow @14:44:27.38 PS.seek 0.599ml @14:44:29.87 PRV.dial :waste2 @14:44:30.74 PS.reconfigure PSconfig @14:44:30.82 PS.seek :empty @14:44:37.81 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:44:37.91 Hand.open @14:44:38.34 Elbow.seek FlushPuck::Garage @14:44:40.39 Elbow.reconfigure LooseElbow @14:44:40.52 Forearm.seek :garage @14:44:41.55 Hand.close @14:44:42.47 Forearm.seek :retract @14:44:43.50 Elbow.reconfigure TightElbow @14:44:43.59 Elbow.seek CC @14:44:46.48 Elbow.reconfigure LooseElbow @14:44:46.60 Forearm.reconfigure TightForearm @14:44:46.68 Forearm.seek CC @14:44:47.96 CC.seek :closed @14:44:52.30 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.26 @14:44:52.41 Hand.open @14:44:53.28 Forearm.reconfigure LooseForearm @14:44:53.37 Forearm.seek :retract @14:44:54.45 Clearing PML for modified lysate @14:44:54.46 CTV.dial :air,avoiding: :intake @14:44:54.51 PRV.dial :waste2 @14:44:54.55 PTV.dial :PRV,avoiding: :puck @14:44:55.43 delay 2 seconds @14:44:57.45 PTV.dial :PRVmixing,avoiding: :puck @14:44:58.32 delay 2 seconds @14:45:00.34 PSV.dial :PML @14:45:00.36 delay 2 seconds @14:45:02.41 PRV.dial :air @14:45:03.27 delay 2 seconds @14:45:05.35 PS.seek 1.499ml @14:45:21.84 delay 5 seconds @14:45:26.88 PS.seek 2.5ml @14:45:38.18 PSV.dial :air @14:45:38.72 PS.seek 0ml @14:46:05.46 mixing lysate with speDil @14:46:05.47 PSV.dial :PML @14:46:06.04 CBV.dial :PRV @14:46:06.05 PRV.dial :air @14:46:06.07 delay 2 seconds @14:46:08.12 PRV.dial :speDil @14:46:08.65 delay 2 seconds @14:46:10.73 PS.reconfigure PSslow1 @14:46:10.82 PS.seek 0.225ml @14:46:34.49 delay 5 seconds @14:46:39.51 PRV.dial CBV @14:46:40.40 delay 2 seconds @14:46:42.48 PS.seek 0.475ml @14:47:08.21 delay 5 seconds @14:47:13.24 PRV.dial :air @14:47:13.81 delay 2 seconds @14:47:15.84 PS.seek 0.965ml @14:48:05.84 delay 10 seconds @14:48:15.87 PRV.dial CBV @14:48:16.40 CTV.dial :air,avoiding: :intake @14:48:16.41 PSV.dial :air @14:48:16.96 PS.reconfigure PSconfig @14:48:17.04 PS.seek 2.5ml @14:48:33.78 delay 3 seconds @14:48:36.80 PSV.dial PTV @14:48:37.79 PTV.dial :PRV,avoiding: :puck @14:48:38.66 PS.seek 0ml @14:49:05.37 PSV.dial :air @14:49:06.24 PS.seek 2.5ml @14:49:32.68 delay 3 seconds @14:49:35.70 PSV.dial PTV @14:49:36.57 PTV.dial :PRV,avoiding: :puck @14:49:36.60 PS.seek 0ml @14:50:03.12 Pushing modified lysate into AIV Injection Loop @14:50:03.13 CBV.dial AIV @14:50:03.18 AIV.dial :bypass @14:50:03.22 PRV.dial CBV @14:50:03.26 PSV.dial :air @14:50:04.15 PS.seek 2.5ml @14:50:30.56 delay 3 seconds @14:50:33.58 PSV.dial :PML @14:50:34.12 PTV.dial :PRVmixing,avoiding: :puck @14:50:35.06 PS.reconfigure PSslow1 @14:50:35.15 PS.seek 1.74ml @14:51:52.81 delay 5 seconds @14:51:57.84 AIV.dial :sample @14:51:58.55 AWV.select :waste @14:51:58.75 PS.reconfigure PSslow2 @14:51:58.83 PS.seek 1.22ml @14:53:46.37 delay 15 seconds @14:54:01.40 AIV.dial :bypass @14:54:02.52 PS.reconfigure PSconfig @14:54:02.61 PS.seek 0ml @14:54:15.97 PSV.dial :air @14:54:16.52 PS.seek 2.5ml @14:54:42.90 PSV.dial :PML @14:54:43.46 PS.seek 0ml @14:55:10.12 AIV.dial :coilToMFB @14:55:10.13 AWV.select :air @14:55:10.35 Extracting Nucleic Acid from AIV @14:55:10.38
Beginning samplePassD @14:55:10.43 SPE.hold 2:46:40,55C @14:55:10.58 ASV.dial :SPEdelivery @14:55:10.67
AWV.select :air @14:55:10.68 AIV.dial :bypass @14:55:10.73 PSV.dial PSR @14:55:11.00 delay 20 seconds @14:55:12.09
Pulling 1.5ml of PSR.killMFB @14:55:12.10 PSR.select :killMFB @14:55:12.29 PS.seek 1.5ml @14:55:29.37 delay 5 seconds @14:55:31.02 primeLysateINT @14:55:31.03 AWV.select :air @14:55:31.08 AIV.dial :coilToMFB @14:55:31.12 ATV.dial AIV @14:55:33.05 AS.reconfigure ASslow1 @14:55:33.14 AS.seek 0.299ml @14:55:34.39
PSV.dial :waste1 @14:55:34.96 PS.seek 0ml @14:55:52.16 PSV.dial PSR @14:55:52.73 PSR.select :air @14:55:52.88 PS.seek 2.5ml @14:56:20.87 PRV.dial CBV @14:56:20.90
CBV.dial PRV @14:56:20.95 PSV.dial :mixloop @14:56:22.33 CTV.dial :air,avoiding: :intake @14:56:22.34 PTV.dial :PRVmixing @14:56:22.42 PS.seek 0ml @14:56:33.74 delay 10 seconds @14:56:43.77 ATV.dial :air2 @14:56:44.67 AS.seek 0.329ml @14:56:50.53
PSV.dial PSR @14:56:51.01 ATV.dial :waste2 @14:56:51.65 AS.reconfigure ASnorm @14:56:51.73 AS.seek :empty @14:56:51.90
Pulling 1.5ml of PSR.flush PSR.select :flush @14:56:52.09 PS.seek 1.5ml @14:57:08.82 Lysate primed! @14:57:08.83 AFV.select :holdingCoil @14:57:08.86 ATV.dial :air2 @14:57:09.28
delay 5 seconds @14:57:09.58 AS.reconfigure ASslow1 @14:57:09.67 AS.seek 0.069ml @14:57:14.30
PSV.dial :waste1 @14:57:14.87 PS.seek 0ml @14:57:24.08 delay 3 seconds @14:57:27.10 ATV.dial :AIV @14:57:28.02 AS.seek 0.469ml @14:57:32.00
PSV.dial PSR @14:57:32.58 PSR.select :air @14:57:32.72 PS.seek 2.5ml @14:58:00.75 PRV.dial CBV @14:58:00.78
CBV.dial PRV @14:58:00.83 PSV.dial :mixloop @14:58:02.09 CTV.dial :air,avoiding: :intake @14:58:02.10 PTV.dial :PRVmixing @14:58:02.17 PS.seek 0ml @14:58:30.12 PSV.dial PSR @14:58:31.42 Pulling 1.5ml of PSR.flush PSR.select :flush @14:58:31.61 PS.seek 1.5ml @14:58:48.53 delay 5 seconds @14:58:48.76 delay 5 seconds @14:58:53.55
PSV.dial :waste1 @14:58:53.82 Solid Phase Extraction Heater at 48C @14:58:53.84 ATV.dial ASV @14:58:54.14
PS.seek 0ml @14:58:54.69 ASV.dial :SPEdelivery @14:58:54.87 AS.seek 0.041ml @14:59:10.94
PSV.dial PSR @14:59:11.52 PSR.select :air @14:59:11.68 PS.seek 2.5ml @14:59:39.09 PRV.dial CBV @14:59:39.12
CBV.dial PRV @14:59:39.18 PSV.dial :mixloop @14:59:40.41 CTV.dial :air,avoiding: :intake @14:59:40.43 PTV.dial :PRVmixing @14:59:40.51 PS.seek 0ml @15:00:08.00 PSV.dial PSR @15:00:09.28 Pulling 1.5ml of PSR.flush PSR.select :flush @15:00:09.47 PS.seek 1.5ml @15:00:21.19 delay 10 seconds @15:00:26.06
delay 5 seconds @15:00:31.08 PSV.dial :waste1 @15:00:31.21 rinseLysateLineINT @15:00:31.22 AFV.select :carrierSolution @15:00:31.38 delay 2 seconds @15:00:31.65
PS.seek 0ml @15:00:33.41 AS.reconfigure ASconfig @15:00:33.49 AS.seek 1ml @15:00:48.31
PSV.dial PSR @15:00:48.86 PSR.select :air @15:00:49.02 PS.seek 2.5ml @15:01:05.73 Session Ended @15:01:16.16 PRV.dial CBV @15:01:16.20
CBV.dial PRV @15:01:16.22 PSV.dial :mixloop @15:01:17.56 CTV.dial :air,avoiding: :intake @15:01:17.59 PTV.dial :PRVmixing @15:01:17.68 PS.reconfigure PSslow @15:01:17.84 PS.seek 1.2ml @15:01:28.90 delay 1 second @15:01:29.92 AFV.select :holdingCoil @15:01:30.05 delay 1 second @15:01:31.07 ATV.dial AIV @15:01:31.74 AIV.dial :coilToMFB @15:01:31.75 AWV.select :waste @15:01:31.90 AS.reconfigure ASslow0 @15:01:31.96 AS.seek 0ml @15:01:36.65 Session Begun @15:01:44.76
PSV.dial :waste1 @15:01:46.31 PS.reconfigure PSconfig @15:01:46.40 PS.seek 0ml @15:01:46.83 -> exit @15:01:46.85 Session Ended @15:01:59.75
PSV.dial :air @15:02:00.96 PS.seek 2.5ml @15:02:25.21 Session Begun @15:02:27.75
PSV.dial :mixloop @15:02:28.29 PS.reconfigure PSslow @15:02:28.37 PS.seek 0ml @15:03:12.91 ATV.dial :air2 @15:03:13.63 AS.reconfigure ASslow1 @15:03:13.71 AS.seek 0.05ml @15:03:19.44
PSV.dial :air @15:03:20.00 PS.reconfigure PSconfig @15:03:20.08 PS.seek 2.5ml @15:03:24.08 ATV.dial :AIV @15:03:25.06 AS.seek 0.01ml @15:03:33.43 ATV.dial :waste @15:03:35.13 AS.seek :empty @15:03:37.62 ethanol wash @15:03:37.63 ATV.dial :air2 @15:03:38.97 AS.reconfigure ASslow2 @15:03:39.06 AS.seek 0.009ml @15:03:46.97
PSV.dial :mixloop @15:03:47.51 PS.reconfigure PSslow @15:03:47.59 PS.seek 0ml @15:03:49.35 delay 3 seconds @15:03:52.37 Pulling 0.4ml of Ethanol Wash @15:03:52.38 ATV.dial :ethanol @15:03:53.27 AS.reconfigure ASslow1 @15:03:53.35 AS.seek 0.409ml @15:04:38.75
Cleaning Collection Syringe @15:04:38.76 CSV.dial CSR @15:04:39.37 Pulling 4ml of CSR.flush CSR.select :flush @15:04:39.55 CS.seek 4ml @15:04:51.22 delay 5 seconds @15:04:56.31 CSR.select :air @15:04:56.47 CS.seek 10ml @15:05:12.73 CSV.dial CTV @15:05:12.76
CTV.dial CSV @15:05:14.10 delay 3 seconds @15:05:14.42
CBV.dial :waste1 @15:05:14.99 CS.seek 0ml @15:05:17.12 ATV.dial :air1 @15:05:17.55 delay 3 seconds @15:05:20.63 AS.seek 0.434ml @15:05:25.95 delay 3 seconds @15:05:28.97 ATV.dial :ethanol @15:05:29.61 AS.seek 0.419ml @15:05:32.94 delay 3 seconds @15:05:35.96 ATV.dial ASV @15:05:37.33 AS.seek 0.092ml @15:05:41.54
CSV.dial CSR @15:05:42.27 Pulling 4ml of CSR.flush @15:05:42.28 CSR.select :flush @15:05:42.46 CS.seek 4ml @15:05:53.76 delay 5 seconds @15:05:58.78 CSR.select :air @15:05:58.94 CS.seek 10ml @15:06:15.32 CSV.dial CTV @15:06:15.35
CTV.dial CSV @15:06:15.90 CBV.dial :waste1 @15:06:15.93 CS.seek 0ml @15:06:42.60 Garaging FlushPuck in Collection Clamp @15:06:42.71 Elbow.reconfigure TightElbow @15:06:42.78 Elbow.seek CC @15:06:43.02 Elbow.reconfigure LooseElbow @15:06:43.15 Forearm.seek CC @15:06:43.40 delay 3 seconds @15:06:44.15
Hand.close @15:06:44.87 CC.seek :open @15:06:46.42 ATV.dial :waste2 @15:06:47.24 AS.reconfigure ASconfig @15:06:47.32 AS.seek :empty @15:06:49.29
Forearm.seek :retract @15:06:50.34 Elbow.reconfigure TightElbow @15:06:50.42 Elbow.seek :garage @15:06:52.99 ATV.dial :air2 @15:06:53.41 AS.reconfigure ASslow1 @15:06:53.52 AS.seek 1ml @15:06:53.66
Elbow.reconfigure LooseElbow @15:06:53.79 Forearm.reconfigure TightForearm @15:06:53.86 Forearm.seek :garage @15:06:55.07 Hand.open @15:06:55.93 Forearm.reconfigure LooseForearm @15:06:56.02 Forearm.seek :retract @15:06:57.07 Whole Cell Archive sampling 1500ml @15:06:57.09 Loading wcr puck from tube 2 into Collection Clamp @15:06:57.38 SC.reconfigure SCtight @15:06:57.49 SC.seek 2 @15:06:59.76 SC.reconfigure SCloose @15:07:00.00 Elbow.reconfigure TightElbow @15:07:00.09 Elbow.seek 2 @15:07:02.27 Forearm.reconfigure TightForearm @15:07:02.36 Forearm.seek 2 + 700 counts @15:07:03.48 SE.reconfigure SEpick @15:07:03.59 SE.seek beamBreak @15:07:07.30 SE.configure SEconfig @15:07:07.49 Elbow.seek 2 @15:07:07.71 Forearm.seek 2 + 300 counts @15:07:07.99 Forearm.reconfigure LooseForearm @15:07:08.08 Hand.close @15:07:08.89 SE.seek :down @15:07:09.13 Forearm.seek :retract @15:07:10.09 Elbow.seek CC @15:07:11.74 Elbow.reconfigure LooseElbow @15:07:11.89 Forearm.reconfigure TightForearm @15:07:11.96 Forearm.seek CC @15:07:12.06 SE.coast @15:07:13.25 CC.seek :closed @15:07:17.50 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.34 @15:07:17.51 Hand.open @15:07:18.43 Forearm.reconfigure LooseForearm @15:07:18.51 Forearm.seek :retract @15:07:19.54 SC.coast @15:07:19.61 collectSample 1500ml w/bubble point of 55psi @15:07:19.63 Priming 7ml @15:07:19.65 Gate.power :sampler,:ON @15:07:19.71 Intake.select :close @15:07:19.74 Exhaust.select :close @15:07:19.80 SS.hold @15:07:20.05 Calibrating pressure sensors @15:07:20.07 CTV.dial :air @15:07:21.68 SSV.dial :spit,avoiding: :exhaust @15:07:21.71 SS.reconfigure SSfast @15:07:21.82 SS.seek 5ml @15:07:24.90 SSV.dial CBV @15:07:24.93 CBV.dial SSV @15:07:26.61 delay 3.8 seconds @15:07:30.53 SSV.dial :spit @15:07:32.09 SS.seek 0ml @15:07:34.72 SSV.dial CBV @15:07:36.31 CTV.dial :intake @15:07:37.20 Waiting for external sampler to finish priming... @15:07:37.21 delayUntil "15:12:19.71" @15:10:15.15 delay 5 seconds @15:10:20.17 ATV.dial ASV @15:10:21.32 ASV.dial :SPEdelivery @15:10:21.35 AS.seek 0.02ml @15:12:19.72 SSV.dial CBV @15:12:19.75 CBV.dial SSV @15:12:19.80 Allowing 40s for 7.0ml of remaining 7.0ml @15:12:19.84 Intake.select :open @15:12:22.06 Intake @29.7psia, vacuum from 20.0 to 24.0psi @15:12:22.08 SS.reconfigure SSinhale.with maxSpeed: 150 @15:12:22.19 SS.seek 7ml @15:12:28.27 Stroke completed -- waiting for pressure to equalize ... @15:12:37.96 Intake.select :close @15:12:38.16 SSV.dial :exhaust @15:12:39.08 Exhaust.select :open @15:12:39.23 SS.reconfigure SSexhale @15:12:39.31 SS.seek 0ml @15:12:43.10 Exhaust.select :close @15:12:43.25 Sampling 1500.0ml @15:12:43.29 Intake.select :close @15:12:43.31 Exhaust.select :close @15:12:43.33 SS.reconfigure SSconfig @15:12:43.45 SS.hold @15:12:43.70 Calibrating pressure sensors @15:12:43.71 CTV.dial :air @15:12:44.63 SSV.dial :spit,avoiding: :exhaust @15:12:45.55 SS.reconfigure SSfast @15:12:45.64 SS.seek 5ml @15:12:48.68 SSV.dial CBV @15:12:48.71 CBV.dial SSV @15:12:50.30 delay 3.8 seconds @15:12:54.22 SSV.dial :spit @15:12:55.78 SS.seek 0ml @15:12:58.33 SSV.dial CBV @15:12:59.91 CTV.dial :intake @15:13:00.84 SSV.dial CBV @15:13:00.87 CBV.dial SSV @15:13:00.92 Allowing 130s for 25.0ml of remaining 1500.0ml @15:13:00.96 Intake.select :open @15:13:03.17 Intake @29.8psia, vacuum from 20.0 to 24.0psi @15:13:03.19 SS.reconfigure SSinhale.with maxSpeed: 150 @15:13:03.30 SS.seek 25ml @15:13:07.93 Vacuum too high -- waiting up to 40s @15:13:08.92 Intake @25.7psia, vacuum from 19.2 to 23.2psi @15:13:08.95 SS.reconfigure SSinhale.with maxSpeed: 135 @15:13:09.05 SS.seek 25ml @15:13:20.63 Stroke completed -- waiting for pressure to equalize ... @15:13:33.37 Intake.select :close @15:13:33.57 SSV.dial :exhaust @15:13:34.49 Exhaust.select :open @15:13:34.64 SS.reconfigure SSexhale @15:13:34.72 SS.seek 0ml @15:13:38.76 delay 5 seconds @15:13:43.78 ATV.dial :air2 @15:13:44.04 Exhaust.select :close @15:13:44.19 SSV.dial CBV @15:13:44.21 CBV.dial SSV @15:13:44.87 AS.seek 0.52ml @15:13:45.15 Allowing 130s for 25.0ml of remaining 1475.0ml @15:13:45.17 Intake.select :open @15:13:47.39 Intake @29.7psia, vacuum from 20.0 to 24.0psi @15:13:47.42 SS.reconfigure SSinhale.with maxSpeed: 135 @15:13:47.53 SS.seek 25ml @15:13:51.90 Vacuum too high -- waiting up to 40s @15:13:52.20 Intake @25.5psia, vacuum from 19.0 to 23.0psi @15:13:52.23 SS.reconfigure SSinhale.with maxSpeed: 122 @15:13:52.33 SS.seek 25ml @15:14:05.21 Stroke completed -- waiting for pressure to equalize ... @15:14:16.26 Intake.select :close @15:14:16.44 SSV.dial :exhaust @15:14:17.35 Exhaust.select :open @15:14:17.50 SS.reconfigure SSexhale @15:14:17.58 SS.seek 0ml @15:14:26.91 Exhaust.select :close @15:14:27.03 SSV.dial CBV @15:14:27.05 CBV.dial SSV @15:14:27.99 Allowing 130s for 25.0ml of remaining 1450.0ml @15:14:28.01 Intake.select :open @15:14:30.25 Intake @29.8psia, vacuum from 20.0 to 24.0psi @15:14:30.28 SS.reconfigure SSinhale.with maxSpeed: 122 @15:14:30.40 SS.seek 25ml @15:14:34.52 Vacuum too high -- waiting up to 40s @15:14:35.30 Intake @25.8psia, vacuum from 19.3 to 23.3psi @15:14:35.33 SS.reconfigure SSinhale.with maxSpeed: 110 @15:14:35.43 SS.seek 25ml @15:14:49.43 Stroke completed -- waiting for pressure to equalize ... @15:14:59.14 Intake.select :close @15:14:59.34 SSV.dial :exhaust @15:15:00.26 Exhaust.select :open @15:15:00.40 SS.reconfigure SSexhale @15:15:00.48 SS.seek 0ml @15:15:09.96 Exhaust.select :close @15:15:10.11 SSV.dial CBV @15:15:10.13 CBV.dial SSV @15:15:11.07 Allowing 130s for 25.0ml of remaining 1425.0ml @15:15:11.09 Intake.select :open @15:15:13.31 Intake @29.9psia, vacuum from 20.0 to 24.0psi @15:15:13.33 SS.reconfigure SSinhale.with maxSpeed: 110 @15:15:13.44 SS.seek 25ml @15:15:17.58 Vacuum too high -- waiting up to 40s @15:15:18.58 Intake @26.2psia, vacuum from 19.7 to 23.7psi @15:15:18.61 SS.reconfigure SSinhale.with maxSpeed: 99 @15:15:18.72 SS.seek 25ml @15:15:25.76 delay 5 seconds @15:15:30.78 ATV.dial ASV @15:15:31.85 ASV.dial :wasteR @15:15:32.25 AS.seek 0.3ml @15:15:34.54 Stroke completed -- waiting for pressure to equalize ... @15:15:42.55 Intake.select :close @15:15:42.74 SSV.dial :exhaust @15:15:43.64 Exhaust.select :open @15:15:43.78 SS.reconfigure SSexhale @15:15:43.86 SS.seek 0ml @15:15:53.14 Exhaust.select :close @15:15:53.26 SSV.dial CBV @15:15:53.28 CBV.dial SSV @15:15:54.24 Allowing 130s for 25.0ml of remaining 1400.0ml @15:15:54.26 Intake.select :open @15:15:56.48 Intake @30.0psia, vacuum from 20.0 to 24.0psi @15:15:56.50 SS.reconfigure SSinhale.with maxSpeed: 99 @15:15:56.61 SS.seek 25ml @15:16:00.86 Vacuum too high -- waiting up to 40s @15:16:01.40 Intake @26.2psia, vacuum from 19.7 to 23.7psi @15:16:01.42 SS.reconfigure SSinhale.with maxSpeed: 89 @15:16:01.52 SS.seek 25ml @15:16:16.77 delay 20 seconds @15:16:18.97 Stroke completed -- waiting for pressure to equalize ... @15:16:26.24 Intake.select :close @15:16:26.44 SSV.dial :exhaust @15:16:27.35 Exhaust.select :open @15:16:27.50 SS.reconfigure SSexhale @15:16:27.58 SS.seek 0ml @15:16:36.50 Exhaust.select :close @15:16:36.65 SSV.dial CBV @15:16:36.67 CBV.dial SSV @15:16:36.80 AS.seek 0.07ml @15:16:37.61 Allowing 130s for 25.0ml of remaining 1375.0ml @15:16:37.63 Intake.select :open @15:16:39.85 Intake @30.1psia, vacuum from 20.0 to 24.0psi @15:16:39.87 SS.reconfigure SSinhale.with maxSpeed: 89 @15:16:40.00 SS.seek 25ml @15:16:43.98 Vacuum too high -- waiting up to 40s @15:16:44.28 Intake @26.0psia, vacuum from 19.5 to 23.5psi @15:16:44.31 SS.reconfigure SSinhale.with maxSpeed: 80 @15:16:44.41 SS.seek 25ml @15:17:03.63 Stroke completed -- waiting for pressure to equalize ... @15:17:09.19 Intake.select :close @15:17:09.37 SSV.dial :exhaust @15:17:10.27 Exhaust.select :open @15:17:10.41 SS.reconfigure SSexhale @15:17:10.49 SS.seek 0ml @15:17:19.91 Exhaust.select :close @15:17:20.07 SSV.dial CBV @15:17:20.09 CBV.dial SSV @15:17:21.02 Allowing 130s for 25.0ml of remaining 1350.0ml @15:17:21.04 Intake.select :open @15:17:23.26 Intake @30.0psia, vacuum from 20.0 to 24.0psi @15:17:23.28 SS.reconfigure SSinhale.with maxSpeed: 80 @15:17:23.41 SS.seek 25ml @15:17:23.43 delay 20 seconds @15:17:28.42 Vacuum too high -- waiting up to 40s @15:17:28.95 Intake @26.1psia, vacuum from 19.6 to 23.6psi @15:17:28.98 SS.reconfigure SSinhale.with maxSpeed: 72 @15:17:29.09 SS.seek 25ml @15:17:43.47 ATV.dial :waste2 @15:17:44.40 AS.reconfigure ASconfig @15:17:44.48 AS.seek :empty @15:17:48.89 Elution @15:17:48.90 ATV.dial :air1 @15:17:49.85 Stroke completed -- waiting for pressure to equalize ... @15:17:50.13 AS.reconfigure ASslow1 @15:17:50.21 AS.seek 0.049ml @15:17:54.07 Intake.select :close @15:17:54.27 SSV.dial :exhaust @15:17:55.18 Exhaust.select :open @15:17:55.33 SS.reconfigure SSexhale @15:17:55.41 SS.seek 0ml @15:18:00.63 delay 3 seconds @15:18:03.65 ATV.dial :ElutionWater @15:18:04.40 AS.seek 0.109ml @15:18:05.00 Exhaust.select :close @15:18:05.16 SSV.dial CBV @15:18:05.18 CBV.dial SSV @15:18:06.14 Allowing 130s for 25.0ml of remaining 1325.0ml @15:18:06.16 Intake.select :open @15:18:08.39 Intake @30.0psia, vacuum from 20.0 to 24.0psi @15:18:08.41 SS.reconfigure SSinhale.with maxSpeed: 72 @15:18:08.53 SS.seek 25ml @15:18:14.17 Vacuum too high -- waiting up to 40s @15:18:14.70 Intake @26.7psia, vacuum from 20.0 to 24.0psi @15:18:14.73 SS.reconfigure SSinhale.with maxSpeed: 65 @15:18:14.85 SS.seek 25ml @15:18:16.84 delay 3 seconds @15:18:19.86 ATV.dial ASV,via: :air2 @15:18:22.10 ASV.dial :SPEelution @15:18:22.74 AFV.select :carrierSolution @15:18:22.93 AS.reconfigure ASnorm @15:18:23.01 AS.seek 0.759ml @15:18:37.35 Stroke completed -- waiting for pressure to equalize ... @15:18:39.85 Intake.select :close @15:18:40.05 SSV.dial :exhaust @15:18:40.94 Exhaust.select :open @15:18:41.09 SS.reconfigure SSexhale @15:18:41.18 SS.seek 0ml @15:18:50.69 Exhaust.select :close @15:18:50.85 SSV.dial CBV @15:18:50.87 CBV.dial SSV @15:18:51.79 Allowing 130s for 25.0ml of remaining 1300.0ml @15:18:51.81 Intake.select :open @15:18:54.03 Intake @30.1psia, vacuum from 20.0 to 24.0psi @15:18:54.05 SS.reconfigure SSinhale.with maxSpeed: 65 @15:18:54.16 SS.seek 25ml @15:18:56.11 delay 3 seconds @15:18:59.13 AFV.select :holdingCoil @15:18:59.29 AS.reconfigure ASslow1 @15:18:59.37 AS.seek 0.474ml @15:19:01.48 Vacuum too high -- waiting up to 40s @15:19:02.02 Intake @26.9psia, vacuum from 20.0 to 24.0psi @15:19:02.04 SS.reconfigure SSinhale.with maxSpeed: 59 @15:19:02.15 SS.seek 25ml @15:19:25.32 Stroke completed -- waiting for pressure to equalize ... @15:19:27.21 Intake.select :close @15:19:27.40 SSV.dial :exhaust @15:19:28.30 Exhaust.select :open @15:19:28.44 SS.reconfigure SSexhale @15:19:28.52 SS.seek 0ml @15:19:37.44 Exhaust.select :close @15:19:37.56 SSV.dial CBV @15:19:37.58 CBV.dial SSV @15:19:38.52 Allowing 130s for 25.0ml of remaining 1275.0ml @15:19:38.54 Intake.select :open @15:19:40.77 Intake @30.2psia, vacuum from 20.0 to 24.0psi @15:19:40.79 SS.reconfigure SSinhale.with maxSpeed: 59 @15:19:40.90 SS.seek 25ml @15:19:56.99 delay 5 seconds @15:20:02.07 AS.reconfigure ASslow2 @15:20:02.16 AS.seek 0.439ml @15:20:11.39 Stroke completed -- waiting for pressure to equalize ... @15:20:13.76 Intake.select :close @15:20:13.94 SSV.dial :exhaust @15:20:14.85 Exhaust.select :open @15:20:14.99 SS.reconfigure SSexhale @15:20:15.08 SS.seek 0ml @15:20:24.39 Exhaust.select :close @15:20:24.55 SSV.dial CBV @15:20:24.57 CBV.dial SSV @15:20:25.50 Allowing 130s for 25.0ml of remaining 1250.0ml @15:20:25.52 Intake.select :open @15:20:27.74 Intake @30.0psia, vacuum from 20.0 to 24.0psi @15:20:27.76 SS.reconfigure SSinhale.with maxSpeed: 59 @15:20:27.87 SS.seek 25ml @15:20:37.79 delay 1:00 @15:20:41.33 Vacuum too high -- waiting up to 40s @15:20:41.63 Intake @26.7psia, vacuum from 20.0 to 24.0psi @15:20:41.66 SS.reconfigure SSinhale.with maxSpeed: 53 @15:20:41.77 SS.seek 25ml @15:21:00.77 Stroke completed -- waiting for pressure to equalize ... @15:21:02.66 Intake.select :close @15:21:02.85 SSV.dial :exhaust @15:21:03.77 Exhaust.select :open @15:21:03.92 SS.reconfigure SSexhale @15:21:04.00 SS.seek 0ml @15:21:13.33 Exhaust.select :close @15:21:13.49 SSV.dial CBV @15:21:13.51 CBV.dial SSV @15:21:14.44 Allowing 130s for 25.0ml of remaining 1225.0ml @15:21:14.46 Intake.select :open @15:21:16.69 Intake @30.1psia, vacuum from 20.0 to 24.0psi @15:21:16.71 SS.reconfigure SSinhale.with maxSpeed: 53 @15:21:16.82 SS.seek 25ml @15:21:37.82 pushing eluate to holding coil @15:21:37.93 AS.seek 0.279ml @15:21:50.22 Stroke completed -- waiting for pressure to equalize ... @15:21:52.11 Intake.select :close @15:21:52.30 SSV.dial :exhaust @15:21:53.21 Exhaust.select :open @15:21:53.37 SS.reconfigure SSexhale @15:21:53.45 SS.seek 0ml @15:22:02.94 Exhaust.select :close @15:22:03.09 SSV.dial CBV @15:22:03.11 CBV.dial SSV @15:22:04.05 Allowing 130s for 25.0ml of remaining 1200.0ml @15:22:04.07 Intake.select :open @15:22:06.29 Intake @30.0psia, vacuum from 20.0 to 24.0psi @15:22:06.32 SS.reconfigure SSinhale.with maxSpeed: 53 @15:22:06.43 SS.seek 25ml @15:22:40.41 Stroke completed -- waiting for pressure to equalize ... @15:22:42.30 Intake.select :close @15:22:42.49 SSV.dial :exhaust @15:22:43.39 Exhaust.select :open @15:22:43.53 SS.reconfigure SSexhale @15:22:43.61 SS.seek 0ml @15:22:52.97 Exhaust.select :close @15:22:53.09 SSV.dial CBV @15:22:53.11 CBV.dial SSV @15:22:54.05 Allowing 130s for 25.0ml of remaining 1175.0ml @15:22:54.07 Intake.select :open @15:22:56.30 Intake @30.1psia, vacuum from 20.0 to 24.0psi @15:22:56.32 SS.reconfigure SSinhale.with maxSpeed: 53 @15:22:56.43 SS.seek 25ml @15:23:30.36 Stroke completed -- waiting for pressure to equalize ... @15:23:33.34 Intake.select :close @15:23:33.56 SSV.dial :exhaust @15:23:34.47 Exhaust.select :open @15:23:34.62 SS.reconfigure SSexhale @15:23:34.71 SS.seek 0ml @15:23:44.19 Exhaust.select :close @15:23:44.35 SSV.dial CBV @15:23:44.37 CBV.dial SSV @15:23:45.32 Allowing 130s for 25.0ml of remaining 1150.0ml @15:23:45.34 Intake.select :open @15:23:47.56 Intake @29.9psia, vacuum from 20.0 to 24.0psi @15:23:47.58 SS.reconfigure SSinhale.with maxSpeed: 53 @15:23:47.69 SS.seek 25ml @15:24:19.04 delay 30 seconds @15:24:21.66 Stroke completed -- waiting for pressure to equalize ... @15:24:23.55 Intake.select :close @15:24:23.74 SSV.dial :exhaust @15:24:24.63 Exhaust.select :open @15:24:24.77 SS.reconfigure SSexhale @15:24:24.85 SS.seek 0ml @15:24:34.25 Exhaust.select :close @15:24:34.41 SSV.dial CBV @15:24:34.43 CBV.dial SSV @15:24:35.36 Allowing 130s for 25.0ml of remaining 1125.0ml @15:24:35.38 Intake.select :open @15:24:37.61 Intake @30.0psia, vacuum from 20.0 to 24.0psi @15:24:37.63 SS.reconfigure SSinhale.with maxSpeed: 53 @15:24:37.74 SS.seek 25ml @15:24:49.07 ATV.dial :waste,via: :air3 @15:24:50.90 AS.reconfigure ASconfig @15:24:50.99 AS.seek :empty @15:25:07.40 Parking Bleach in LysateSource line and SPEcolumn @15:25:07.41 ATV.dial :air3 @15:25:08.31 AS.reconfigure ASslow1 @15:25:08.39 AS.seek 0.029ml @15:25:11.72 Stroke completed -- waiting for pressure to equalize ... @15:25:13.62 Intake.select :close @15:25:13.81 SSV.dial :exhaust @15:25:14.73 Exhaust.select :open @15:25:14.76 ATV.dial :ElutionWater @15:25:14.95 SS.reconfigure SSexhale @15:25:15.03 SS.seek 0ml @15:25:15.46 AS.seek 0.059ml @15:25:21.76 ATV.dial :air3 @15:25:22.43 AS.seek 0.069ml @15:25:24.46 Exhaust.select :close @15:25:24.61 SSV.dial CBV @15:25:24.63 CBV.dial SSV @15:25:24.77 delay 2 seconds @15:25:25.60 Allowing 130s for 25.0ml of remaining 1100.0ml @15:25:25.62 Intake.select :open @15:25:26.79 ATV.dial :bleach @15:25:27.41 AS.seek 0.769ml @15:25:27.84 Intake @29.8psia, vacuum from 20.0 to 24.0psi @15:25:27.86 SS.reconfigure SSinhale.with maxSpeed: 53 @15:25:27.97 SS.seek 25ml @15:26:01.75 Stroke completed -- waiting for pressure to equalize ... @15:26:04.25 Intake.select :close @15:26:04.44 SSV.dial :exhaust @15:26:05.36 Exhaust.select :open @15:26:05.50 SS.reconfigure SSexhale @15:26:05.58 SS.seek 0ml @15:26:14.94 Exhaust.select :close @15:26:15.10 SSV.dial CBV @15:26:15.12 CBV.dial SSV @15:26:16.57 Allowing 130s for 25.0ml of remaining 1075.0ml @15:26:16.59 Intake.select :open @15:26:18.81 Intake @29.8psia, vacuum from 20.0 to 24.0psi @15:26:18.83 SS.reconfigure SSinhale.with maxSpeed: 53 @15:26:18.95 SS.seek 25ml @15:26:52.66 Stroke completed -- waiting for pressure to equalize ... @15:26:55.17 Intake.select :close @15:26:55.35 SSV.dial :exhaust @15:26:56.25 Exhaust.select :open @15:26:56.39 SS.reconfigure SSexhale @15:26:56.47 SS.seek 0ml @15:27:06.07 Exhaust.select :close @15:27:06.23 SSV.dial CBV @15:27:06.25 CBV.dial SSV @15:27:07.19 Allowing 130s for 25.0ml of remaining 1050.0ml @15:27:07.21 Intake.select :open @15:27:09.43 Intake @29.9psia, vacuum from 20.0 to 24.0psi @15:27:09.44 SS.reconfigure SSinhale.with maxSpeed: 53 @15:27:09.56 SS.seek 25ml @15:27:43.47 Stroke completed -- waiting for pressure to equalize ... @15:27:45.36 Intake.select :close @15:27:45.55 SSV.dial :exhaust @15:27:46.44 Exhaust.select :open @15:27:46.60 SS.reconfigure SSexhale @15:27:46.68 SS.seek 0ml @15:27:48.52 delay 3 seconds @15:27:51.54 ATV.dial :air3 @15:27:52.14 delay 3 seconds @15:27:55.21 AS.seek 0.794ml @15:27:55.58 Exhaust.select :close @15:27:55.73 SSV.dial CBV @15:27:55.76 CBV.dial SSV @15:27:56.69 Allowing 130s for 25.0ml of remaining 1025.0ml @15:27:56.71 Intake.select :open @15:27:58.93 Intake @29.9psia, vacuum from 20.0 to 24.0psi @15:27:58.94 SS.reconfigure SSinhale.with maxSpeed: 53 @15:27:59.06 SS.seek 25ml @15:28:00.54 delay 3 seconds @15:28:03.56 ATV.dial :bleach @15:28:04.23 AS.seek 0.779ml @15:28:07.53 delay 3 seconds @15:28:10.55 ATV.dial :AIV @15:28:11.89 AS.reconfigure ASconfig @15:28:11.97 AS.seek 0.015ml @15:28:33.36 Stroke completed -- waiting for pressure to equalize ... @15:28:35.86 Intake.select :close @15:28:36.03 SSV.dial :exhaust @15:28:36.92 Exhaust.select :open @15:28:37.07 SS.reconfigure SSexhale @15:28:37.15 SS.seek 0ml @15:28:46.38 Exhaust.select :close @15:28:46.50 SSV.dial CBV @15:28:46.52 CBV.dial SSV @15:28:47.44 Allowing 130s for 25.0ml of remaining 1000.0ml @15:28:47.46 Intake.select :open @15:28:49.67 Intake @29.9psia, vacuum from 20.0 to 24.0psi @15:28:49.69 SS.reconfigure SSinhale.with maxSpeed: 53 @15:28:49.80 SS.seek 25ml @15:28:56.20 ATV.dial :waste @15:28:57.81 AS.seek :empty @15:28:59.07 ATV.dial :air3 @15:28:59.79 AS.reconfigure ASslow1 @15:28:59.87 AS.seek 0.009ml @15:29:02.25 delay 2 seconds @15:29:04.27 ATV.dial :bleach @15:29:04.76 AS.seek 0.579ml @15:29:23.45 Stroke completed -- waiting for pressure to equalize ... @15:29:25.95 Intake.select :close @15:29:26.15 SSV.dial :exhaust @15:29:27.05 Exhaust.select :open @15:29:27.19 SS.reconfigure SSexhale @15:29:27.27 SS.seek 0ml @15:29:36.56 Exhaust.select :close @15:29:36.72 SSV.dial CBV @15:29:36.74 CBV.dial SSV @15:29:37.67 Allowing 130s for 25.0ml of remaining 975.0ml @15:29:37.69 Intake.select :open @15:29:39.92 Intake @29.8psia, vacuum from 20.0 to 24.0psi @15:29:39.94 SS.reconfigure SSinhale.with maxSpeed: 53 @15:29:40.05 SS.seek 25ml @15:30:13.75 Stroke completed -- waiting for pressure to equalize ... @15:30:16.25 Intake.select :close @15:30:16.45 SSV.dial :exhaust @15:30:17.35 Exhaust.select :open @15:30:17.50 SS.reconfigure SSexhale @15:30:17.59 SS.seek 0ml @15:30:26.49 Exhaust.select :close @15:30:26.61 SSV.dial CBV @15:30:26.63 CBV.dial SSV @15:30:27.55 Allowing 130s for 25.0ml of remaining 950.0ml @15:30:27.57 Intake.select :open @15:30:29.79 Intake @29.9psia, vacuum from 20.0 to 24.0psi @15:30:29.81 SS.reconfigure SSinhale.with maxSpeed: 53 @15:30:29.92 SS.seek 25ml @15:30:59.64 delay 3 seconds @15:31:02.66 ATV.dial :air3 @15:31:03.26 delay 3 seconds @15:31:04.24 Stroke completed -- waiting for pressure to equalize ... @15:31:06.34 AS.seek 0.604ml @15:31:06.75 Intake.select :close @15:31:06.94 SSV.dial :exhaust @15:31:07.83 Exhaust.select :open @15:31:07.98 SS.reconfigure SSexhale @15:31:08.06 SS.seek 0ml @15:31:11.68 delay 3 seconds @15:31:14.70 ATV.dial :bleach @15:31:15.34 AS.seek 0.589ml @15:31:17.36 Exhaust.select :close @15:31:17.51 SSV.dial CBV @15:31:17.53 CBV.dial SSV @15:31:18.47 Allowing 130s for 25.0ml of remaining 925.0ml @15:31:18.49 Intake.select :open @15:31:18.68 delay 3 seconds @15:31:20.71 Intake @30.0psia, vacuum from 20.0 to 24.0psi @15:31:20.73 SS.reconfigure SSinhale.with maxSpeed: 53 @15:31:20.84 SS.seek 25ml @15:31:21.70 ATV.dial :ASV @15:31:22.83 ASV.dial :wasteR @15:31:23.48 AS.seek 0ml @15:31:54.44 Stroke completed -- waiting for pressure to equalize ... @15:31:56.94 Intake.select :close @15:31:57.12 SSV.dial :exhaust @15:31:58.02 Exhaust.select :open @15:31:58.17 SS.reconfigure SSexhale @15:31:58.25 SS.seek 0ml @15:32:08.20 Exhaust.select :close @15:32:08.36 SSV.dial CBV @15:32:08.38 CBV.dial SSV @15:32:09.31 Allowing 130s for 25.0ml of remaining 900.0ml @15:32:09.33 Intake.select :open @15:32:11.55 Intake @30.0psia, vacuum from 20.0 to 24.0psi @15:32:11.57 SS.reconfigure SSinhale.with maxSpeed: 53 @15:32:11.68 SS.seek 25ml @15:32:45.72 Stroke completed -- waiting for pressure to equalize ... @15:32:48.22 Intake.select :close @15:32:48.42 SSV.dial :exhaust @15:32:49.33 Exhaust.select :open @15:32:49.47 SS.reconfigure SSexhale @15:32:49.56 SS.seek 0ml @15:32:58.85 Exhaust.select :close @15:32:59.01 SSV.dial CBV @15:32:59.03 CBV.dial SSV @15:32:59.97 Allowing 130s for 25.0ml of remaining 875.0ml @15:32:59.99 Intake.select :open @15:33:02.21 Intake @30.1psia, vacuum from 20.0 to 24.0psi @15:33:02.23 SS.reconfigure SSinhale.with maxSpeed: 53 @15:33:02.34 SS.seek 25ml @15:33:22.29 delay 1:00 @15:33:35.89 Stroke completed -- waiting for pressure to equalize ... @15:33:38.41 Intake.select :close @15:33:38.61 SSV.dial :exhaust @15:33:39.52 Exhaust.select :open @15:33:39.66 SS.reconfigure SSexhale @15:33:39.74 SS.seek 0ml @15:33:49.00 Exhaust.select :close @15:33:49.16 SSV.dial CBV @15:33:49.18 CBV.dial SSV @15:33:50.11 Allowing 130s for 25.0ml of remaining 850.0ml @15:33:50.13 Intake.select :open @15:33:52.36 Intake @30.0psia, vacuum from 20.0 to 24.0psi @15:33:52.37 SS.reconfigure SSinhale.with maxSpeed: 53 @15:33:52.49 SS.seek 25ml @15:34:22.32 AFV.select :carrierSolution @15:34:22.46 delay 2 seconds @15:34:24.49 AS.reconfigure ASconfig @15:34:24.57 AS.seek 1ml @15:34:26.08 Stroke completed -- waiting for pressure to equalize ... @15:34:28.59 Intake.select :close @15:34:28.76 SSV.dial :exhaust @15:34:29.67 Exhaust.select :open @15:34:29.82 SS.reconfigure SSexhale @15:34:29.90 SS.seek 0ml @15:34:39.14 Exhaust.select :close @15:34:39.26 SSV.dial CBV @15:34:39.28 CBV.dial SSV @15:34:40.22 Allowing 130s for 25.0ml of remaining 825.0ml @15:34:40.24 Intake.select :open @15:34:42.46 Intake @30.0psia, vacuum from 20.0 to 24.0psi @15:34:42.48 SS.reconfigure SSinhale.with maxSpeed: 53 @15:34:42.59 SS.seek 25ml @15:35:02.14 Vacuum too high -- waiting up to 40s @15:35:02.44 Intake @26.8psia, vacuum from 20.0 to 24.0psi @15:35:02.46 SS.reconfigure SSinhale.with maxSpeed: 48 @15:35:02.57 SS.seek 25ml @15:35:18.49 Stroke completed -- waiting for pressure to equalize ... @15:35:20.38 Intake.select :close @15:35:20.57 SSV.dial :exhaust @15:35:21.49 Exhaust.select :open @15:35:22.14 SS.reconfigure SSexhale @15:35:22.22 SS.seek 0ml @15:35:22.37 delay 1 second @15:35:23.39 AFV.select :holdingCoil @15:35:23.52 delay 1 second @15:35:24.55 ATV.dial ASV @15:35:24.56 ASV.dial :wasteR @15:35:24.60 AS.reconfigure ASslow1 @15:35:24.70 AS.seek 0ml @15:35:31.14 Exhaust.select :close @15:35:31.29 SSV.dial CBV @15:35:31.31 CBV.dial SSV @15:35:32.25 Allowing 130s for 25.0ml of remaining 800.0ml @15:35:32.27 Intake.select :open @15:35:34.50 Intake @30.0psia, vacuum from 20.0 to 24.0psi @15:35:34.52 SS.reconfigure SSinhale.with maxSpeed: 48 @15:35:34.64 SS.seek 25ml @15:36:12.33 Stroke completed -- waiting for pressure to equalize ... @15:36:14.83 Intake.select :close @15:36:15.02 SSV.dial :exhaust @15:36:15.92 Exhaust.select :open @15:36:16.07 SS.reconfigure SSexhale @15:36:16.15 SS.seek 0ml @15:36:25.35 Exhaust.select :close @15:36:25.51 SSV.dial CBV @15:36:25.53 CBV.dial SSV @15:36:26.47 Allowing 130s for 25.0ml of remaining 775.0ml @15:36:26.49 Intake.select :open @15:36:28.72 Intake @30.0psia, vacuum from 20.0 to 24.0psi @15:36:28.74 SS.reconfigure SSinhale.with maxSpeed: 48 @15:36:28.85 SS.seek 25ml @15:37:05.97 Stroke completed -- waiting for pressure to equalize ... @15:37:08.47 Intake.select :close @15:37:08.66 SSV.dial :exhaust @15:37:09.56 Exhaust.select :open @15:37:09.71 SS.reconfigure SSexhale @15:37:09.79 SS.seek 0ml @15:37:18.88 Exhaust.select :close @15:37:19.00 SSV.dial CBV @15:37:19.02 CBV.dial SSV @15:37:19.97 Allowing 130s for 25.0ml of remaining 750.0ml @15:37:19.99 Intake.select :open @15:37:22.21 Intake @30.1psia, vacuum from 20.0 to 24.0psi @15:37:22.22 SS.reconfigure SSinhale.with maxSpeed: 48 @15:37:22.34 SS.seek 25ml @15:37:59.97 Stroke completed -- waiting for pressure to equalize ... @15:38:02.47 Intake.select :close @15:38:02.65 SSV.dial :exhaust @15:38:03.55 Exhaust.select :open @15:38:03.71 SS.reconfigure SSexhale @15:38:03.79 SS.seek 0ml @15:38:13.19 Exhaust.select :close @15:38:13.35 SSV.dial CBV @15:38:13.37 CBV.dial SSV @15:38:14.33 Allowing 130s for 25.0ml of remaining 725.0ml @15:38:14.35 Intake.select :open @15:38:16.57 Intake @29.9psia, vacuum from 20.0 to 24.0psi @15:38:16.59 SS.reconfigure SSinhale.with maxSpeed: 48 @15:38:16.71 SS.seek 25ml @15:38:46.12 delay 20 seconds @15:38:54.03 Stroke completed -- waiting for pressure to equalize ... @15:38:56.53 Intake.select :close @15:38:56.70 SSV.dial :exhaust @15:38:58.10 Exhaust.select :open @15:38:58.24 SS.reconfigure SSexhale @15:38:58.32 SS.seek 0ml @15:39:06.14 ATV.dial :air3 @15:39:07.61 Exhaust.select :close @15:39:07.78 AS.seek 0.59ml @15:39:07.86 SSV.dial CBV @15:39:07.93 CBV.dial SSV @15:39:08.81 Allowing 130s for 25.0ml of remaining 700.0ml @15:39:08.83 Intake.select :open @15:39:11.05 Intake @30.0psia, vacuum from 20.0 to 24.0psi @15:39:11.07 SS.reconfigure SSinhale.with maxSpeed: 48 @15:39:11.20 SS.seek 25ml @15:39:48.02 Stroke completed -- waiting for pressure to equalize ... @15:39:50.52 Intake.select :close @15:39:50.71 SSV.dial :exhaust @15:39:51.61 Exhaust.select :open @15:39:51.75 SS.reconfigure SSexhale @15:39:51.83 SS.seek 0ml @15:40:01.39 Exhaust.select :close @15:40:01.55 SSV.dial CBV @15:40:01.57 CBV.dial SSV @15:40:02.53 Allowing 130s for 25.0ml of remaining 675.0ml @15:40:02.55 Intake.select :open @15:40:04.77 Intake @29.9psia, vacuum from 20.0 to 24.0psi @15:40:04.80 SS.reconfigure SSinhale.with maxSpeed: 48 @15:40:04.91 SS.seek 25ml @15:40:42.20 Stroke completed -- waiting for pressure to equalize ... @15:40:44.09 Intake.select :close @15:40:44.27 SSV.dial :exhaust @15:40:45.14 Exhaust.select :open @15:40:45.28 SS.reconfigure SSexhale @15:40:45.36 SS.seek 0ml @15:40:54.72 Exhaust.select :close @15:40:54.88 SSV.dial CBV @15:40:54.90 CBV.dial SSV @15:40:55.81 Allowing 130s for 25.0ml of remaining 650.0ml @15:40:55.83 Intake.select :open @15:40:58.05 Intake @29.8psia, vacuum from 20.0 to 24.0psi @15:40:58.08 SS.reconfigure SSinhale.with maxSpeed: 48 @15:40:58.19 SS.seek 25ml @15:41:06.76 delay 3 seconds @15:41:09.78 ATV.dial :ASV @15:41:11.30 ASV.dial :wasteR @15:41:11.42 AS.seek 0.09ml @15:41:35.13 Stroke completed -- waiting for pressure to equalize ... @15:41:37.63 Intake.select :close @15:41:37.83 SSV.dial :exhaust @15:41:38.72 Exhaust.select :open @15:41:38.88 SS.reconfigure SSexhale @15:41:38.96 SS.seek 0ml @15:41:48.39 Exhaust.select :close @15:41:48.55 SSV.dial CBV @15:41:48.57 CBV.dial SSV @15:41:49.49 Allowing 130s for 25.0ml of remaining 625.0ml @15:41:49.51 Intake.select :open @15:41:51.71 Intake @29.9psia, vacuum from 20.0 to 24.0psi @15:41:51.73 SS.reconfigure SSinhale.with maxSpeed: 48 @15:41:51.84 SS.seek 25ml @15:42:29.19 Stroke completed -- waiting for pressure to equalize ... @15:42:31.69 Intake.select :close @15:42:32.34 SSV.dial :exhaust @15:42:33.27 Exhaust.select :open @15:42:33.42 SS.reconfigure SSexhale @15:42:33.50 SS.seek 0ml @15:42:42.78 Exhaust.select :close @15:42:42.94 SSV.dial CBV @15:42:42.96 CBV.dial SSV @15:42:43.89 Allowing 130s for 25.0ml of remaining 600.0ml @15:42:43.91 Intake.select :open @15:42:46.14 Intake @30.0psia, vacuum from 20.0 to 24.0psi @15:42:46.16 SS.reconfigure SSinhale.with maxSpeed: 48 @15:42:46.27 SS.seek 25ml @15:42:52.30 delay 30 seconds @15:43:22.33 ATV.dial :extSrc @15:43:23.14 Stroke completed -- waiting for pressure to equalize ... @15:43:23.22 delay 3 seconds @15:43:25.65 Intake.select :close @15:43:25.85 SSV.dial :exhaust @15:43:26.23 ATV.dial :ElutionWater @15:43:26.77 Exhaust.select :open @15:43:26.91 SS.reconfigure SSexhale @15:43:26.99 SS.seek 0ml @15:43:27.41 AS.seek 0.12ml @15:43:33.75 ATV.dial :air3 @15:43:34.40 AS.seek 0.13ml @15:43:36.24 Exhaust.select :close @15:43:36.39 SSV.dial CBV @15:43:36.41 CBV.dial SSV @15:43:36.75 delay 2 seconds @15:43:37.33 Allowing 130s for 25.0ml of remaining 575.0ml @15:43:37.35 Intake.select :open @15:43:38.77 ATV.dial :bleach @15:43:39.28 AS.seek 0.23ml @15:43:39.58 Intake @29.9psia, vacuum from 20.0 to 24.0psi @15:43:39.60 SS.reconfigure SSinhale.with maxSpeed: 48 @15:43:39.71 SS.seek 25ml @15:43:59.77 delay 3 seconds @15:44:02.79 ATV.dial :air3 @15:44:03.37 delay 3 seconds @15:44:06.44 AS.seek 0.255ml @15:44:11.79 delay 3 seconds @15:44:14.81 ATV.dial :bleach @15:44:15.45 AS.seek 0.24ml @15:44:16.56 Stroke completed -- waiting for pressure to equalize ... @15:44:18.78 delay 3 seconds @15:44:19.07 Intake.select :close @15:44:19.25 SSV.dial :exhaust @15:44:20.16 Exhaust.select :open @15:44:20.31 SS.reconfigure SSexhale @15:44:20.39 SS.seek 0ml @15:44:21.80 ATV.dial :ASV @15:44:22.92 ASV.dial :SPEdelivery @15:44:23.33 AFV.select :carrierSolution @15:44:23.45 delay 2 seconds @15:44:25.49 AS.reconfigure ASconfig @15:44:25.57 AS.seek 0.5ml @15:44:29.86 Exhaust.select :close @15:44:30.02 SSV.dial CBV @15:44:30.04 CBV.dial SSV @15:44:30.99 Allowing 130s for 25.0ml of remaining 550.0ml @15:44:31.01 Intake.select :open @15:44:33.24 Intake @30.0psia, vacuum from 20.0 to 24.0psi @15:44:33.26 SS.reconfigure SSinhale.with maxSpeed: 48 @15:44:33.37 SS.seek 25ml @15:44:40.84 delay 1 second @15:44:41.86 AFV.select :holdingCoil @15:44:42.02 AS.reconfigure ASslow1 @15:44:42.10 AS.seek 0.17ml @15:45:10.77 Stroke completed -- waiting for pressure to equalize ... @15:45:13.75 Intake.select :close @15:45:13.95 SSV.dial :exhaust @15:45:14.86 Exhaust.select :open @15:45:15.02 SS.reconfigure SSexhale @15:45:15.10 SS.seek 0ml @15:45:24.33 Exhaust.select :close @15:45:24.49 SSV.dial CBV @15:45:24.51 CBV.dial SSV @15:45:25.44 Allowing 130s for 25.0ml of remaining 525.0ml @15:45:25.46 Intake.select :open @15:45:27.67 Intake @30.0psia, vacuum from 20.0 to 24.0psi @15:45:27.69 SS.reconfigure SSinhale.with maxSpeed: 48 @15:45:27.80 SS.seek 25ml @15:45:48.79 SPE.hold 2:46:40,95C @15:45:48.86 delay 3:00 @15:46:04.78 Stroke completed -- waiting for pressure to equalize ... @15:46:07.28 Intake.select :close @15:46:07.47 SSV.dial :exhaust @15:46:08.41 Exhaust.select :open @15:46:08.55 SS.reconfigure SSexhale @15:46:08.63 SS.seek 0ml @15:46:18.06 Exhaust.select :close @15:46:18.22 SSV.dial CBV @15:46:18.24 CBV.dial SSV @15:46:19.19 Allowing 130s for 25.0ml of remaining 500.0ml @15:46:19.21 Intake.select :open @15:46:21.04 Session Ended @15:46:21.09 Session Ended @15:46:21.44 Intake @29.7psia, vacuum from 20.0 to 24.0psi @15:46:21.46 SS.reconfigure SSinhale.with maxSpeed: 48 @15:46:21.54 SS.seek 25ml @15:46:47.58 Vacuum too high -- waiting up to 40s @15:46:47.87 Intake @26.7psia, vacuum from 20.0 to 24.0psi @15:46:47.89 SS.reconfigure SSinhale.with maxSpeed: 43 @15:46:47.97 SS.seek 25ml @15:47:00.17 Stroke completed -- waiting for pressure to equalize ... @15:47:02.68 Intake.select :close @15:47:02.85 SSV.dial :exhaust @15:47:03.77 Exhaust.select :open @15:47:03.90 SS.reconfigure SSexhale @15:47:03.96 SS.seek 0ml @15:47:13.28 Exhaust.select :close @15:47:13.40 SSV.dial CBV @15:47:13.42 CBV.dial SSV @15:47:14.34 Allowing 130s for 25.0ml of remaining 475.0ml @15:47:14.36 Intake.select :open @15:47:16.54 Intake @29.8psia, vacuum from 20.0 to 24.0psi @15:47:16.55 SS.reconfigure SSinhale.with maxSpeed: 43 @15:47:16.63 SS.seek 25ml @15:47:57.78 Stroke completed -- waiting for pressure to equalize ... @15:48:00.28 Intake.select :close @15:48:00.44 SSV.dial :exhaust @15:48:01.38 Exhaust.select :open @15:48:01.51 SS.reconfigure SSexhale @15:48:01.57 SS.seek 0ml @15:48:11.02 Exhaust.select :close @15:48:11.14 SSV.dial CBV @15:48:11.16 CBV.dial SSV @15:48:12.09 Allowing 130s for 25.0ml of remaining 450.0ml @15:48:12.11 Intake.select :open @15:48:14.30 Intake @29.8psia, vacuum from 20.0 to 24.0psi @15:48:14.31 SS.reconfigure SSinhale.with maxSpeed: 43 @15:48:14.39 SS.seek 25ml @15:48:48.88 AFV.select :carrierSolution @15:48:48.99 delay 2 seconds @15:48:51.02 AS.reconfigure ASconfig @15:48:51.55 AS.seek 1ml @15:48:55.91 Stroke completed -- waiting for pressure to equalize ... @15:48:58.41 Intake.select :close @15:48:58.58 SSV.dial :exhaust @15:48:59.48 Exhaust.select :open @15:48:59.61 SS.reconfigure SSexhale @15:48:59.67 SS.seek 0ml @15:49:09.11 Exhaust.select :close @15:49:09.24 SSV.dial CBV @15:49:09.26 CBV.dial SSV @15:49:10.20 Allowing 130s for 25.0ml of remaining 425.0ml @15:49:10.22 Intake.select :open @15:49:12.40 Intake @29.8psia, vacuum from 20.0 to 24.0psi @15:49:12.42 SS.reconfigure SSinhale.with maxSpeed: 43 @15:49:12.50 SS.seek 25ml @15:49:39.55 delay 1 second @15:49:40.57 AFV.select :holdingCoil @15:49:40.67 delay 1 second @15:49:41.69 ASV.dial :wasteD @15:49:42.93 AS.seek 0ml @15:49:53.91 Stroke completed -- waiting for pressure to equalize ... @15:49:56.41 Intake.select :close @15:49:56.58 SSV.dial :exhaust @15:49:57.50 Exhaust.select :open @15:49:57.63 SS.reconfigure SSexhale @15:49:57.69 SS.seek 0ml @15:50:06.95 Exhaust.select :close @15:50:07.08 SSV.dial CBV @15:50:07.10 CBV.dial SSV @15:50:08.05 Allowing 130s for 25.0ml of remaining 400.0ml @15:50:08.07 Intake.select :open @15:50:10.26 Intake @29.7psia, vacuum from 20.0 to 24.0psi @15:50:10.28 SS.reconfigure SSinhale.with maxSpeed: 43 @15:50:10.35 SS.seek 25ml @15:50:40.72 Priming Eluate @15:50:40.73 AFV.select :carrierSolution @15:50:40.85 delay 2 seconds @15:50:42.88 AS.seek 0.5ml @15:50:51.48 Stroke completed -- waiting for pressure to equalize ... @15:50:54.59 Intake.select :close @15:50:54.75 SSV.dial :exhaust @15:50:55.67 Exhaust.select :open @15:50:55.80 SS.reconfigure SSexhale @15:50:55.86 SS.seek 0ml @15:51:05.22 Exhaust.select :close @15:51:05.34 SSV.dial CBV @15:51:05.36 CBV.dial SSV @15:51:06.30 Allowing 130s for 25.0ml of remaining 375.0ml @15:51:06.32 Intake.select :open @15:51:08.51 Intake @29.9psia, vacuum from 20.0 to 24.0psi @15:51:08.53 SS.reconfigure SSinhale.with maxSpeed: 43 @15:51:08.60 SS.seek 25ml @15:51:11.91 delay 1 second @15:51:12.93 AFV.select :holdingCoil @15:51:13.03 ATV.dial ASV @15:51:13.04 ASV.dial :SPErecovery @15:51:13.70 Swish #1 of 2 @15:51:13.78 AS.reconfigure ASslow0 @15:51:13.84 AS.seek 0.6ml @15:51:24.33 AS.seek 0.5ml @15:51:34.70 Swish #2 of 2 @15:51:34.80 AS.seek 0.6ml @15:51:45.25 AS.seek 0.5ml @15:51:49.55 Stroke completed -- waiting for pressure to equalize ... @15:51:52.54 Intake.select :close @15:51:52.70 SSV.dial :exhaust @15:51:53.61 Exhaust.select :open @15:51:53.74 SS.reconfigure SSexhale @15:51:53.80 SS.seek 0ml @15:51:55.66 delay 3 seconds @15:51:58.73 AS.reconfigure ASslow1 @15:51:58.79 AS.seek 0.684ml @15:52:03.06 Exhaust.select :close @15:52:03.19 SSV.dial CBV @15:52:03.21 CBV.dial SSV @15:52:04.15 Allowing 130s for 25.0ml of remaining 350.0ml @15:52:04.17 Intake.select :open @15:52:06.35 Intake @30.0psia, vacuum from 20.0 to 24.0psi @15:52:06.37 SS.reconfigure SSinhale.with maxSpeed: 43 @15:52:06.45 SS.seek 25ml @15:52:36.09 delay 3 seconds @15:52:39.11 Eluate primed @15:52:39.12 ATV.dial :waste2 @15:52:39.92 AS.reconfigure ASnorm @15:52:39.98 AS.seek 0ml @15:52:47.42 Stroke completed -- waiting for pressure to equalize ... @15:52:50.63 Intake.select :close @15:52:50.89 SSV.dial :exhaust @15:52:51.78 Exhaust.select :open @15:52:51.91 SS.reconfigure SSexhale @15:52:51.97 SS.seek 0ml @15:53:01.17 Session Begun @15:53:01.44 Exhaust.select :close @15:53:01.59 SSV.dial CBV @15:53:01.61 CBV.dial SSV @15:53:02.55 Allowing 130s for 25.0ml of remaining 325.0ml @15:53:02.57 Intake.select :open @15:53:04.77 Intake @29.9psia, vacuum from 20.0 to 24.0psi @15:53:04.79 SS.reconfigure SSinhale.with maxSpeed: 43 @15:53:04.88 SS.seek 25ml @15:53:14.78 SPE.coast @15:53:14.88 ASV.dial :SPErecovery @15:53:14.90 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118aug2115h1500ml.pcr @15:53:14.93 Building PCR Slug from ASV with MM2 mix, PP4 primer @15:53:14.96 Priming with carrier @15:53:14.98 ATV.dial ARV @15:53:16.02 ARV.dial :carrier @15:53:16.38 AS.seek 0.5ml @15:53:41.97 AFV.select :carrierSolution @15:53:42.10 delay 2 seconds @15:53:44.13 AS.reconfigure ASconfig @15:53:44.20 AS.seek 1ml @15:53:46.34 Stroke completed -- waiting for pressure to equalize ... @15:53:49.45 Intake.select :close @15:53:49.63 SSV.dial :exhaust @15:53:50.53 Exhaust.select :open @15:53:50.67 SS.reconfigure SSexhale @15:53:50.74 SS.seek 0ml @15:53:59.94 Exhaust.select :close @15:54:00.06 SSV.dial CBV @15:54:00.08 CBV.dial SSV @15:54:01.00 Allowing 130s for 25.0ml of remaining 300.0ml @15:54:01.02 Intake.select :open @15:54:03.24 Intake @30.0psia, vacuum from 20.0 to 24.0psi @15:54:03.26 SS.reconfigure SSinhale.with maxSpeed: 43 @15:54:03.36 SS.seek 25ml @15:54:13.25 delay 1 second @15:54:14.27 AFV.select :holdingCoil @15:54:14.38 delay 1 second @15:54:15.40 ATV.dial :waste @15:54:16.99 AS.reconfigure ASnorm @15:54:17.07 AS.seek :empty @15:54:44.39 Stroke completed -- waiting for pressure to equalize ... @15:54:47.99 Intake.select :close @15:54:48.18 SSV.dial :exhaust @15:54:49.08 Exhaust.select :open @15:54:49.22 SS.reconfigure SSexhale @15:54:49.29 SS.seek 0ml @15:54:58.77 Exhaust.select :close @15:54:58.91 SSV.dial CBV @15:54:58.96 CBV.dial SSV @15:54:59.80 Allowing 130s for 25.0ml of remaining 275.0ml @15:54:59.82 Intake.select :open @15:55:02.02 Intake @30.0psia, vacuum from 20.0 to 24.0psi @15:55:02.04 SS.reconfigure SSinhale.with maxSpeed: 43 @15:55:02.13 SS.seek 25ml @15:55:07.83 AFV.select :carrierSolution @15:55:07.96 delay 2 seconds @15:55:09.99 AS.reconfigure ASconfig @15:55:10.06 AS.seek 0.5ml @15:55:39.19 delay 1 second @15:55:40.21 AFV.select :holdingCoil @15:55:40.34 Building Slug @15:55:40.35 ATV.dial :ARV @15:55:42.08 delay 2 seconds @15:55:43.59 Stroke completed -- waiting for pressure to equalize ... @15:55:44.10 ARV.dial :air1 @15:55:44.52 delay 2 seconds @15:55:46.18 Intake.select :close @15:55:46.36 SSV.dial :exhaust @15:55:46.59 AS.reconfigure ASslow2 @15:55:46.67 AS.seek 0.506ml @15:55:47.27 Exhaust.select :open @15:55:47.41 SS.reconfigure SSexhale @15:55:47.48 SS.seek 0ml @15:55:52.86 delay 2 seconds @15:55:54.88 ARV.dial :MM2 @15:55:56.46 delay 2 seconds @15:55:56.73 Exhaust.select :close @15:55:56.87 SSV.dial CBV @15:55:56.89 CBV.dial SSV @15:55:57.81 Allowing 130s for 25.0ml of remaining 250.0ml @15:55:57.83 Intake.select :open @15:55:58.48 Adding MM2 @15:55:58.58 AS.seek 0.524ml @15:56:00.06 Intake @29.9psia, vacuum from 20.0 to 24.0psi @15:56:00.08 SS.reconfigure SSinhale.with maxSpeed: 43 @15:56:00.18 SS.seek 25ml @15:56:16.84 delay 25 seconds @15:56:41.08 Stroke completed -- waiting for pressure to equalize ... @15:56:41.86 ARV.dial :PP4 @15:56:42.33 Adding PP4 @15:56:42.35 delay 2 seconds @15:56:44.43 AS.seek 0.53ml @15:56:44.82 Intake.select :close @15:56:45.00 SSV.dial :exhaust @15:56:45.91 Exhaust.select :open @15:56:46.05 SS.reconfigure SSexhale @15:56:46.13 SS.seek 0ml @15:56:50.63 delay 10 seconds @15:56:55.48 Exhaust.select :close @15:56:55.62 SSV.dial CBV @15:56:55.64 CBV.dial SSV @15:56:56.56 Allowing 130s for 25.0ml of remaining 225.0ml @15:56:56.58 Intake.select :open @15:56:58.78 Intake @29.8psia, vacuum from 20.0 to 24.0psi @15:56:58.79 SS.reconfigure SSinhale.with maxSpeed: 43 @15:56:58.89 SS.seek 25ml @15:57:00.65 ARV.dial :air3 @15:57:01.59 adding ARV air @15:57:01.61 delay 2 seconds @15:57:03.71 AS.seek 0.536ml @15:57:10.03 delay 5 seconds @15:57:15.05 ARV.dial :carrier @15:57:16.42 delay 7 seconds @15:57:23.51 AS.seek 0.594ml @15:57:40.30 Stroke completed -- waiting for pressure to equalize ... @15:57:43.41 Intake.select :close @15:57:43.60 SSV.dial :exhaust @15:57:44.50 Exhaust.select :open @15:57:44.64 SS.reconfigure SSexhale @15:57:44.71 SS.seek 0ml @15:57:54.00 Exhaust.select :close @15:57:54.14 SSV.dial CBV @15:57:54.16 CBV.dial SSV @15:57:55.09 Allowing 130s for 25.0ml of remaining 200.0ml @15:57:55.11 Intake.select :open @15:57:57.32 Intake @29.8psia, vacuum from 20.0 to 24.0psi @15:57:57.34 SS.reconfigure SSinhale.with maxSpeed: 43 @15:57:57.44 SS.seek 25ml @15:58:22.08 delay 5 seconds @15:58:27.10 ATV.dial :ASV @15:58:27.55 delay 2 seconds @15:58:29.63 AS.seek 0.6ml @15:58:36.06 delay 4 seconds @15:58:38.33 Stroke completed -- waiting for pressure to equalize ... @15:58:40.15 ATV.dial ARV @15:58:40.74 delay 2 seconds @15:58:41.54 Intake.select :close @15:58:41.72 SSV.dial :exhaust @15:58:42.62 Exhaust.select :open @15:58:42.76 SS.reconfigure SSexhale @15:58:42.83 ARV.dial :carrier @15:58:42.86 SS.seek 0ml @15:58:42.90 delay 2 seconds @15:58:45.00 AS.seek 0.65ml @15:58:52.12 Exhaust.select :close @15:58:52.26 SSV.dial CBV @15:58:52.28 CBV.dial SSV @15:58:53.19 Allowing 130s for 25.0ml of remaining 175.0ml @15:58:53.21 Intake.select :open @15:58:55.40 Intake @29.9psia, vacuum from 20.0 to 24.0psi @15:58:55.42 SS.reconfigure SSinhale.with maxSpeed: 43 @15:58:55.52 SS.seek 25ml @15:59:17.38 Vacuum too high -- waiting up to 40s @15:59:17.69 Intake @27.0psia, vacuum from 20.0 to 24.0psi @15:59:17.71 SS.reconfigure SSinhale.with maxSpeed: 39 @15:59:17.80 SS.seek 25ml @15:59:35.53 delay 4 seconds @15:59:39.11 Stroke completed -- waiting for pressure to equalize ... @15:59:39.55 ATV.dial PCR @15:59:40.20 ASV.dial :waste1 @15:59:41.05 ARV.dial :air1 @15:59:41.47 Positioning Slug by 0.324ml @15:59:41.58 AS.seek 0.326ml @15:59:41.81 Intake.select :close @15:59:41.99 SSV.dial :exhaust @15:59:42.89 Exhaust.select :open @15:59:43.41 SS.reconfigure SSexhale @15:59:43.56 SS.seek 0ml @15:59:52.78 Exhaust.select :close @15:59:52.92 SSV.dial CBV @15:59:52.94 CBV.dial SSV @15:59:53.86 Allowing 130s for 25.0ml of remaining 150.0ml @15:59:53.88 Intake.select :open @15:59:56.09 Intake @29.8psia, vacuum from 20.0 to 24.0psi @15:59:56.11 SS.reconfigure SSinhale.with maxSpeed: 39 @15:59:56.20 SS.seek 25ml @16:00:41.33 Stroke completed -- waiting for pressure to equalize ... @16:00:45.05 Intake.select :close @16:00:45.22 SSV.dial :exhaust @16:00:46.12 Exhaust.select :open @16:00:46.26 SS.reconfigure SSexhale @16:00:46.33 SS.seek 0ml @16:00:55.51 Exhaust.select :close @16:00:55.65 SSV.dial CBV @16:00:55.67 CBV.dial SSV @16:00:56.59 Allowing 130s for 25.0ml of remaining 125.0ml @16:00:56.61 Intake.select :open @16:00:58.82 Intake @30.0psia, vacuum from 20.0 to 24.0psi @16:00:58.84 SS.reconfigure SSinhale.with maxSpeed: 39 @16:00:58.93 SS.seek 25ml @16:01:44.20 Stroke completed -- waiting for pressure to equalize ... @16:01:49.14 Intake.select :close @16:01:49.32 SSV.dial :exhaust @16:01:50.22 Exhaust.select :open @16:01:50.36 SS.reconfigure SSexhale @16:01:50.43 SS.seek 0ml @16:01:59.28 Exhaust.select :close @16:01:59.42 SSV.dial CBV @16:01:59.44 CBV.dial SSV @16:01:59.66 Session Ended @16:02:00.34 Allowing 130s for 25.0ml of remaining 100.0ml @16:02:00.36 Intake.select :open @16:02:02.55 Intake @29.9psia, vacuum from 20.0 to 24.0psi @16:02:02.56 SS.reconfigure SSinhale.with maxSpeed: 39 @16:02:02.64 SS.seek 25ml @16:02:48.48 Stroke completed -- waiting for pressure to equalize ... @16:03:28.70 SS.reconfigure SSconfig @16:03:28.77 SS.hold @16:03:28.89 Intake.select :close @16:03:29.05 ShallowSampler::Clogged in WCR -- Intake @30psia & Outlet @26psia won't equalize after 1425.0ml @16:03:29.06 SSV.dial :exhaust @16:03:29.95 Exhaust.select :open @16:03:30.08 SS.reconfigure SSexhale @16:03:30.14 SS.seek 0ml @16:03:39.45 Exhaust.select :close @16:03:39.57 Sampled 1425.0ml @16:03:39.59 Drying puck before fixing for 4:00 @16:03:39.61 CBV.dial :waste2b @16:03:40.51 delay 1:00 @16:03:42.58 Gate.power :sampler,:OFF @16:04:40.54 CSV.dial :air @16:04:41.06 CS.reconfigure CSfast @16:04:41.66 CS.seek 10ml @16:05:07.71 delay 5 seconds @16:05:08.25 Pumping wcr puck from tube 2 up to 55psi... @16:05:08.26 CBV.dial SSV @16:05:09.71 CBV.dial between CSV and waste2b @16:05:10.81 CSV.dial between air and CTV @16:05:11.21 CS.seek 7.351ml @16:05:12.73 ATV.dial ARV @16:05:13.63 ASV.dial :PCRlock @16:05:14.02 delay 5 seconds @16:05:19.06 CTV.dial CSV,avoiding: :air @16:05:19.09 CSV.dial CTV @16:05:19.20 Running Tt1 Profile60C300 thermocycling @16:05:19.21 PCR.program Profile60C300 @16:05:19.35 Profile60C300 should complete in < 1:45:21 @16:05:19.36 PCR.run 2:16:41.25 @16:05:20.02 CS.reconfigure CSpressurize @16:05:20.08 CS.seek 0ml @16:05:37.11 CBV.dial :waste2b @16:05:37.48 CS.reconfigure CSconfig @16:05:37.55 CS.seek :empty @16:05:41.12 delay 1:00 @16:06:41.14 CTV.dial :air,avoiding: :intake @16:06:42.70 Fix #1 of 2 (with rnal) for 2:00 @16:06:42.71 CSV.dial CSR @16:06:43.29 Pulling 2ml of CSR.rnal @16:06:43.30 CSR.select :rnal @16:06:43.44 CS.seek 2ml @16:06:49.65 delay 5 seconds @16:06:54.67 CSR.select :air @16:06:54.81 CS.reconfigure CSfast @16:06:54.87 CS.seek 10ml @16:07:16.37 Pumping wcr puck from tube 2 up to 55psi... @16:07:16.38 CBV.dial SSV @16:07:17.82 CBV.dial between CSV and waste2b @16:07:18.92 CSV.dial between air and CTV @16:07:19.63 CS.seek 7.325ml @16:07:27.56 CTV.dial CSV,avoiding: :air @16:07:27.59 CSV.dial CTV @16:07:29.20 CS.reconfigure CSpressurize @16:07:29.27 CS.seek 0ml @16:07:47.52 delay 2:00 @16:09:47.54 CBV.dial :waste2b @16:09:47.91 CS.reconfigure CSconfig @16:09:47.98 CS.seek :empty @16:09:50.09 delay 1:00 @16:10:50.11 CTV.dial :air,avoiding: :intake @16:10:51.64 Fix #2 of 2 (with rnal) for 2:00 @16:10:51.66 CSV.dial CSR @16:10:52.36 Pulling 2ml of CSR.rnal @16:10:52.37 CSR.select :rnal @16:10:52.52 CS.seek 2ml @16:10:58.69 delay 5 seconds @16:11:03.71 CSR.select :air @16:11:03.85 CS.reconfigure CSfast @16:11:03.91 CS.seek 10ml @16:11:25.31 Pumping wcr puck from tube 2 up to 55psi... @16:11:25.32 CBV.dial SSV @16:11:26.76 CBV.dial between CSV and waste2b @16:11:27.87 CSV.dial between air and CTV @16:11:28.57 CS.seek 7.285ml @16:11:36.94 CTV.dial CSV,avoiding: :air @16:11:36.97 CSV.dial CTV @16:11:38.59 CS.reconfigure CSpressurize @16:11:38.66 CS.seek 0ml @16:11:56.05 delay 2:00 @16:13:56.07 CBV.dial :waste2b @16:13:56.44 CS.reconfigure CSconfig @16:13:56.51 CS.seek :empty @16:13:59.41 delay 1:00 @16:14:59.43 CTV.dial :air,avoiding: :intake @16:15:01.05 Storing wcr puck in Collection Clamp to tube 1 @16:15:01.24 SC.reconfigure SCtight @16:15:01.31 SC.seek 1 @16:15:03.47 SC.reconfigure SCloose @16:15:03.69 Elbow.reconfigure TightElbow @16:15:03.75 Elbow.seek 1 @16:15:05.71 Forearm.reconfigure TightForearm @16:15:05.77 Forearm.seek 1 + 700 counts @16:15:06.81 SE.reconfigure SEpick @16:15:06.91 SE.seek beamBreak @16:15:10.08 SE.configure SEconfig @16:15:10.25 Elbow.reconfigure LimpElbow @16:15:10.32 Forearm.reconfigure LooseForearm @16:15:10.38 Forearm.seek 1 @16:15:11.20 Elbow.hold @16:15:11.29 Elbow.reconfigure TightElbow @16:15:11.41 SE.seek 1.04 Pucks below Top Plate @16:15:11.92 Forearm.force -20 @16:15:11.98 Forearm.seek 1 + 333 counts @16:15:12.30 Forearm.reconfigure TightForearm @16:15:12.36 Forearm.seek 1 + 700 counts @16:15:12.74 SE.seek 2.04 Pucks below Top Plate @16:15:13.37 Forearm.reconfigure LooseForearm @16:15:13.43 Forearm.seek :retract @16:15:14.34 Elbow.seek CC @16:15:16.04 Elbow.reconfigure LooseElbow @16:15:16.16 Forearm.seek CC @16:15:17.17 Hand.close @16:15:17.94 CC.seek :open @16:15:22.42 Forearm.seek :retract @16:15:23.42 Elbow.reconfigure TightElbow @16:15:23.49 Elbow.seek 1 @16:15:25.47 Elbow.reconfigure LimpElbow @16:15:25.55 Forearm.seek 1 @16:15:26.53 Elbow.hold @16:15:26.62 Elbow.reconfigure TightElbow @16:15:26.68 Hand.open @16:15:27.56 Forearm.force -20 @16:15:27.61 Forearm.seek 1 + 349 counts @16:15:27.95 Forearm.reconfigure TightForearm @16:15:28.02 Forearm.seek 1 + 700 counts @16:15:28.35 SE.seek :down @16:15:29.48 Forearm.reconfigure LooseForearm @16:15:29.54 Forearm.seek :retract @16:15:30.97 SE.coast @16:15:31.01 SC.coast @16:15:31.07 Starting finalPurge @16:15:31.08 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:15:31.18 Hand.open @16:15:31.56 Elbow.seek FlushPuck::Garage @16:15:33.59 Elbow.reconfigure LooseElbow @16:15:33.71 Forearm.seek :garage @16:15:34.70 Hand.close @16:15:35.56 Forearm.seek :retract @16:15:37.06 Elbow.reconfigure TightElbow @16:15:37.13 Elbow.seek CC @16:15:40.04 Elbow.reconfigure LooseElbow @16:15:40.15 Forearm.reconfigure TightForearm @16:15:40.21 Forearm.seek CC @16:15:41.42 CC.seek :closed @16:15:45.68 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.32 @16:15:45.76 Hand.open @16:15:46.62 Forearm.reconfigure LooseForearm @16:15:46.68 Forearm.seek :retract @16:15:47.67 Flushing the Collection Valve::Manifold @16:15:47.81 CSV.dial CSR @16:15:48.38 Pulling 5ml of CSR.flush CSR.select :flush @16:15:48.53 CS.seek 5ml @16:16:02.44 delay 5 seconds @16:16:07.46 CSR.select :air @16:16:07.63 CS.reconfigure CSfast @16:16:07.69 CS.seek 7.5ml @16:16:15.13 CSV.dial :waste @16:16:16.65 CS.reconfigure CSconfig @16:16:16.72 CS.seek 0ml @16:16:36.88 Flushing lower manifold's waste1 port @16:16:37.02 CSV.dial :flush @16:16:37.65 CS.reconfigure CSfast @16:16:37.72 CS.seek 5ml @16:16:51.54 delay 5 seconds @16:16:56.56 CSV.dial :air @16:16:57.18 CS.seek 7ml @16:17:03.41 CSV.dial CTV @16:17:03.44
CTV.dial CSV @16:17:05.07 CBV.dial :waste1 @16:17:05.95 CS.seek 0ml @16:17:25.04 Flushing lower manifold's waste2b port @16:17:25.18 CSV.dial :flush @16:17:26.06 CS.seek 5ml @16:17:39.76 delay 5 seconds @16:17:44.78 CSV.dial :air @16:17:45.39 CS.seek 7ml @16:17:51.57 CSV.dial CTV @16:17:51.60
CTV.dial CSV @16:17:52.10 CBV.dial :waste2b @16:17:52.99 CS.seek 0ml @16:18:11.69 Flushing lower manifold's waste1 port @16:18:11.70 CSV.dial CSR @16:18:12.30 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @16:18:12.45 CS.reconfigure CSconfig @16:18:12.52 CS.seek 4ml @16:18:23.85 delay 5 seconds @16:18:28.87 CSR.select :air @16:18:29.00 CS.reconfigure CSfast @16:18:29.06 CS.seek 10ml @16:18:45.35 CSV.dial CTV @16:18:45.38
CTV.dial CSV @16:18:45.91 CBV.dial :waste1 @16:18:46.80 CS.seek 0ml @16:19:13.54 Flushing lower manifold's waste2b port @16:19:13.55 CSV.dial CSR @16:19:14.15 Pulling 4ml of CSR.mfbkill @16:19:14.16 CSR.select :mfbkill @16:19:14.31 CS.reconfigure CSconfig @16:19:14.37 CS.seek 4ml @16:19:25.60 delay 5 seconds @16:19:30.62 CSR.select :air @16:19:30.75 CS.reconfigure CSfast @16:19:30.81 CS.seek 10ml @16:19:47.16 CSV.dial CTV @16:19:47.19
CTV.dial CSV @16:19:47.73 CBV.dial :waste2b @16:19:48.63 CS.seek 0ml @16:20:15.20 Killing upper manifold @16:20:15.21 CSV.dial CSR @16:20:15.82 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @16:20:15.96 CS.reconfigure CSconfig @16:20:16.02 CS.seek 4ml @16:20:27.23 delay 5 seconds @16:20:32.25 CSR.select :air @16:20:32.39 CS.reconfigure CSfast @16:20:32.45 CS.seek 10ml @16:20:48.76 CSV.dial CBV @16:20:48.79
CBV.dial CSV @16:20:50.02 CTV.dial :air @16:20:51.58 CS.seek 0ml @16:21:18.13 delay 7 seconds @16:21:25.16 CS.seek 3ml @16:21:33.85 CSV.dial :waste @16:21:34.38 CS.seek 0ml @16:21:43.20 Flushing upper manifold @16:21:43.34 CSV.dial :flush @16:21:43.86 CS.seek 5ml @16:21:57.45 delay 5 seconds @16:22:02.47 CSV.dial :air @16:22:03.05 CS.seek 7ml @16:22:09.07 CSV.dial CBV @16:22:09.10
CBV.dial CSV @16:22:10.31 CTV.dial :air @16:22:10.33 CS.seek 0ml @16:22:29.10 delay 7 seconds @16:22:36.13 CS.seek 3ml @16:22:44.76 CSV.dial :waste @16:22:45.30 CS.seek 0ml @16:22:54.04 CSV.dial :flush @16:22:54.55 CS.seek 3ml @16:23:03.26 delay 5 seconds @16:23:08.28 CSV.dial :air @16:23:09.39 CS.seek 5ml @16:23:15.42 CSV.dial :air @16:23:15.44 CS.seek 0ml @16:23:29.01 delay 7 seconds @16:23:36.04 CS.seek 3ml @16:23:44.64 CSV.dial :waste @16:23:45.52 CS.seek 0ml @16:23:54.17 Flushing lower manifold's waste1 port @16:23:54.31 CSV.dial :flush @16:23:54.87 CS.seek 5ml @16:24:08.54 delay 5 seconds @16:24:13.56 CSV.dial :air @16:24:14.14 CS.seek 7ml @16:24:20.32 CSV.dial CTV @16:24:20.35
CTV.dial CSV @16:24:21.98 CBV.dial :waste1 @16:24:22.52 CS.seek 0ml @16:24:41.32 Flushing lower manifold's waste2b port @16:24:41.46 CSV.dial :flush @16:24:42.35 CS.seek 5ml @16:24:56.11 delay 5 seconds @16:25:01.13 CSV.dial :air @16:25:01.73 CS.seek 7ml @16:25:07.79 CSV.dial CTV @16:25:07.82
CTV.dial CSV @16:25:08.34 CBV.dial :waste2b @16:25:09.23 CS.seek 0ml @16:25:27.83 Garaging FlushPuck in Collection Clamp @16:25:27.92 Elbow.reconfigure TightElbow @16:25:27.98 Elbow.seek CC @16:25:28.21 Elbow.reconfigure LooseElbow @16:25:28.32 Forearm.seek CC @16:25:29.32 Hand.close @16:25:30.07 CC.seek :open @16:25:34.48 Forearm.seek :retract @16:25:35.49 Elbow.reconfigure TightElbow @16:25:35.55 Elbow.seek :garage @16:25:38.87 Elbow.reconfigure LooseElbow @16:25:38.98 Forearm.reconfigure TightForearm @16:25:39.04 Forearm.seek :garage @16:25:40.24 Hand.open @16:25:41.01 Forearm.reconfigure LooseForearm @16:25:41.07 Forearm.seek :retract @16:25:42.10 Starting initialPurge @16:25:42.11 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:25:42.20 Hand.open @16:25:42.51 Elbow.reconfigure TightElbow @16:25:42.57 Elbow.seek FlushPuck::Garage @16:25:42.70 Elbow.reconfigure LooseElbow @16:25:42.81 Forearm.seek :garage @16:25:43.81 Hand.close @16:25:45.12 Forearm.seek :retract @16:25:46.21 Elbow.reconfigure TightElbow @16:25:46.27 Elbow.seek CC @16:25:49.13 Elbow.reconfigure LooseElbow @16:25:49.24 Forearm.reconfigure TightForearm @16:25:49.30 Forearm.seek CC @16:25:50.55 CC.seek :closed @16:25:54.80 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.25 @16:25:54.90 Hand.open @16:25:55.77 Forearm.reconfigure LooseForearm @16:25:55.83 Forearm.seek :retract @16:25:56.82 Flushing the Collection Valve::Manifold @16:25:56.96 CSV.dial CSR @16:25:57.52 Pulling 5ml of CSR.flush @16:25:57.53 CSR.select :flush @16:25:57.68 CS.reconfigure CSconfig @16:25:57.74 CS.seek 5ml @16:26:11.45 delay 5 seconds @16:26:16.47 CSR.select :air @16:26:16.65 CS.reconfigure CSfast @16:26:16.71 CS.seek 7.5ml @16:26:24.04 CSV.dial :waste @16:26:25.56 CS.reconfigure CSconfig @16:26:25.63 CS.seek 0ml @16:26:46.14 Purging external Intake @16:26:46.27 CSV.dial :flush @16:26:46.80 CS.reconfigure CSfast @16:26:46.87 CS.seek 10ml @16:27:13.36 delay 5 seconds @16:27:18.38 CSV.dial CBV @16:27:18.42
CBV.dial CSV @16:27:19.34 CTV.dial :intake @16:27:20.22 Intake.select :open @16:27:20.35 CS.seek 0ml @16:27:47.95 Intake.select :close @16:27:48.07 CTV.dial :air @16:27:48.94 Flushing upper manifold @16:27:49.07 CSV.dial :flush @16:27:49.95 CS.seek 5ml @16:28:03.67 delay 5 seconds @16:28:08.69 CSV.dial :air @16:28:09.26 CS.seek 7ml @16:28:15.30 CSV.dial CBV @16:28:15.33
CBV.dial CSV @16:28:16.66 CTV.dial :air @16:28:16.68 CS.seek 0ml @16:28:35.42 delay 7 seconds @16:28:42.45 CS.seek 3ml @16:28:51.02 CSV.dial :waste @16:28:51.53 CS.seek 0ml @16:29:00.24 CSV.dial :flush @16:29:00.78 CS.seek 3ml @16:29:09.42 delay 5 seconds @16:29:14.44 CSV.dial :air @16:29:15.64 CS.seek 5ml @16:29:21.77 CSV.dial :air @16:29:21.79 CS.seek 0ml @16:29:35.48 delay 7 seconds @16:29:42.51 CS.seek 3ml @16:29:51.08 CSV.dial :waste @16:29:51.95 CS.seek 0ml @16:30:00.61 Garaging FlushPuck in Collection Clamp @16:30:00.70 Elbow.reconfigure TightElbow @16:30:00.76 Elbow.seek CC @16:30:01.00 Elbow.reconfigure LooseElbow @16:30:01.11 Forearm.seek CC @16:30:02.08 Hand.close @16:30:02.83 CC.seek :open @16:30:07.22 Forearm.seek :retract @16:30:08.22 Elbow.reconfigure TightElbow @16:30:08.28 Elbow.seek :garage @16:30:11.36 Elbow.reconfigure LooseElbow @16:30:11.47 Forearm.reconfigure TightForearm @16:30:11.53 Forearm.seek :garage @16:30:12.69 Hand.open @16:30:13.51 Forearm.reconfigure LooseForearm @16:30:13.57 Forearm.seek :retract @16:30:14.61 Completed [mbaPhase] begun 13:12:12 @16:30:14.63 Extending 18tun1 until completion of MFBPCR @17:26:02.24 PCR.off @17:26:03.78 PCR for Tt1 completed -- closed /var/log/esp/Tt118aug2115h1500ml.pcr @17:26:03.86 Cleaning PCR @17:26:03.87 AFV.select :carrierSolution @17:26:04.00 delay 2 seconds @17:26:06.03 AS.reconfigure ASconfig @17:26:06.08 AS.seek 0.5ml @17:26:16.38 delay 1 second @17:26:17.40 AFV.select :holdingCoil @17:26:17.50 ASV.dial :waste1 @17:26:17.89 ATV.dial :air3 @17:26:19.17 AS.reconfigure ASslow1 @17:26:19.23 AS.seek 0.51ml @17:26:21.56 delay 2 seconds @17:26:23.58 ATV.dial :bleach @17:26:24.20 AS.seek 0.81ml @17:27:24.82 delay 3 seconds @17:27:27.84 ATV.dial :air3 @17:27:28.41 delay 3 seconds @17:27:31.48 AS.seek 0.835ml @17:27:36.81 delay 3 seconds @17:27:39.83 ATV.dial :bleach @17:27:40.45 AS.seek 0.82ml @17:27:43.78 delay 3 seconds @17:27:46.80 ARV.dial :waste @17:27:47.43 delay 3 seconds @17:27:50.45 ATV.dial :ARV @17:27:51.34 AS.reconfigure ASconfig @17:27:51.40 AS.seek 0.47ml @17:28:11.80 AFV.select :carrierSolution @17:28:11.91 delay 2 seconds @17:28:13.94 AS.seek 1ml @17:28:44.69 delay 1 second @17:28:45.71 AFV.select :holdingCoil @17:28:45.81 delay 1 second @17:28:46.84 AS.reconfigure ASfast @17:28:46.89 AS.seek 0ml @17:29:14.29 AFV.select :carrierSolution @17:29:14.41 delay 2 seconds @17:29:16.44 AS.reconfigure ASconfig @17:29:16.49 AS.seek 1ml @17:30:14.25 delay 1 second @17:30:15.27 AFV.select :holdingCoil @17:30:15.37 delay 1 second @17:30:16.40 AS.reconfigure ASfast @17:30:16.45 AS.seek 0ml @17:30:43.85 AFV.select :carrierSolution @17:30:43.96 delay 2 seconds @17:30:45.99 AS.reconfigure ASconfig @17:30:46.04 AS.seek 1ml @17:31:43.82 delay 1 second @17:31:44.84 AFV.select :holdingCoil @17:31:44.94 delay 1 second @17:31:45.97 AS.reconfigure ASfast @17:31:46.02 AS.seek 0ml @17:32:13.38 ARV.dial between carrier and air1 @17:32:13.90 ATV.dial :air3 @17:32:15.21 AS.reconfigure ASslow1 @17:32:15.27 AS.seek 0.01ml @17:32:17.58 delay 2 seconds @17:32:19.60 ATV.dial :bleach @17:32:20.22 AS.seek 0.81ml @17:35:01.35 delay 3 seconds @17:35:04.37 ATV.dial :air3 @17:35:04.93 delay 3 seconds @17:35:08.00 AS.seek 0.835ml @17:35:13.31 delay 3 seconds @17:35:16.33 ATV.dial :bleach @17:35:16.96 AS.seek 0.82ml @17:35:20.22 delay 3 seconds @17:35:23.24 ATV.dial PCR @17:35:23.65 AS.reconfigure ASconfig @17:35:23.71 AS.seek :empty @17:36:11.16 PCR.program :bleachProfile @17:36:11.22 PCR program should complete in < 2:45 @17:36:11.23 PCR.run 10:00 @17:39:13.49 PCR.off @17:39:13.59 AFV.select :carrierSolution @17:39:13.70 delay 2 seconds @17:39:15.73 AS.seek 1ml @17:40:13.55 delay 1 second @17:40:14.57 AFV.select :holdingCoil @17:40:14.67 delay 1 second @17:40:15.70 AS.reconfigure ASfast @17:40:15.76 AS.seek 0ml @17:40:43.13 delay 3 seconds @17:40:46.15 AFV.select :carrierSolution @17:40:46.25 delay 2 seconds @17:40:48.28 AS.reconfigure ASconfig @17:40:48.34 AS.seek 1ml @17:41:46.10 delay 1 second @17:41:47.12 AFV.select :holdingCoil @17:41:47.22 delay 1 second @17:41:48.25 AS.reconfigure ASfast @17:41:48.30 AS.seek 0ml @17:42:15.65 delay 3 seconds @17:42:18.67 AFV.select :carrierSolution @17:42:18.77 delay 2 seconds @17:42:20.80 AS.reconfigure ASconfig @17:42:20.86 AS.seek 1ml @17:43:18.63 delay 1 second @17:43:19.65 AFV.select :holdingCoil @17:43:19.75 delay 1 second @17:43:20.78 AS.reconfigure ASfast @17:43:20.83 AS.seek 0ml @17:43:48.18 delay 3 seconds @17:43:51.20 AFV.select :carrierSolution @17:43:51.30 delay 2 seconds @17:43:53.33 AS.reconfigure ASconfig @17:43:53.38 AS.seek 1ml @17:44:51.18 delay 1 second @17:44:52.20 AFV.select :holdingCoil @17:44:52.30 delay 1 second @17:44:53.33 AS.reconfigure ASfast @17:44:53.38 AS.seek 0ml @17:45:20.74 delay 3 seconds @17:45:23.76 AFV.select :carrierSolution @17:45:23.86 delay 2 seconds @17:45:25.89 AS.reconfigure ASconfig @17:45:25.95 AS.seek 1ml @17:46:23.71 delay 1 second @17:46:24.73 AFV.select :holdingCoil @17:46:24.83 delay 1 second @17:46:25.86 AS.reconfigure ASnorm @17:46:25.91 AS.seek 0ml @17:47:16.62 Setting up PCR for IPc -- writing /var/log/esp/IPc18aug2117h1500ml.pcr @17:47:16.64 Building PCR Slug from ASV with MM1 mix, PP1 primer @17:47:16.66 Priming with carrier @17:47:16.68 ATV.dial ARV @17:47:17.32 ARV.dial :carrier @17:47:17.68 AS.seek 0.5ml @17:47:43.23 AFV.select :carrierSolution @17:47:43.35 delay 2 seconds @17:47:45.38 AS.reconfigure ASconfig @17:47:45.44 AS.seek 1ml @17:48:14.46 delay 1 second @17:48:15.48 AFV.select :holdingCoil @17:48:15.58 delay 1 second @17:48:16.60 ATV.dial :waste @17:48:18.27 AS.reconfigure ASnorm @17:48:18.34 AS.seek :empty @17:49:09.09 AFV.select :carrierSolution @17:49:09.20 delay 2 seconds @17:49:11.23 AS.reconfigure ASconfig @17:49:11.28 AS.seek 0.5ml @17:49:40.41 delay 1 second @17:49:41.43 AFV.select :holdingCoil @17:49:41.54 Building Slug @17:49:41.55 ATV.dial :ARV @17:49:43.20 delay 2 seconds @17:49:45.22 ARV.dial :air1 @17:49:45.74 delay 2 seconds @17:49:47.81 AS.reconfigure ASslow2 @17:49:47.87 AS.seek 0.506ml @17:49:54.27 delay 2 seconds @17:49:56.29 ARV.dial :MM1 @17:49:56.91 delay 2 seconds @17:49:58.93 Adding MM1 @17:49:59.02 AS.seek 0.524ml @17:50:17.41 delay 25 seconds @17:50:42.43 ARV.dial :PP1 @17:50:42.84 Adding PP1 @17:50:42.85 delay 2 seconds @17:50:44.93 AS.seek 0.53ml @17:50:51.37 delay 10 seconds @17:51:01.40 ARV.dial :air1 @17:51:01.81 adding ARV air @17:51:01.82 delay 2 seconds @17:51:03.90 AS.seek 0.536ml @17:51:10.34 delay 5 seconds @17:51:15.36 ARV.dial :carrier @17:51:15.77 delay 7 seconds @17:51:22.85 AS.seek 0.594ml @17:52:21.62 delay 5 seconds @17:52:26.64 ATV.dial :ASV @17:52:27.06 delay 2 seconds @17:52:29.13 AS.seek 0.6ml @17:52:35.63 delay 4 seconds @17:52:39.65 ATV.dial ARV @17:52:40.21 delay 2 seconds @17:52:42.23 ARV.dial :carrier @17:52:42.24 delay 2 seconds @17:52:44.32 AS.seek 0.65ml @17:53:35.01 delay 4 seconds @17:53:39.03 ATV.dial PCR @17:53:40.18 ASV.dial :waste1 @17:53:40.19 ARV.dial :air1 @17:53:40.62 Positioning Slug by 0.324ml @17:53:40.72 AS.seek 0.326ml @17:59:07.03 delay 5 seconds @17:59:12.05 ATV.dial ARV @17:59:12.85 ASV.dial :PCRlock @17:59:13.24 delay 5 seconds @17:59:18.26 Running IPc ProfileIPc thermocycling @17:59:18.28 PCR.program ProfileIPc @17:59:18.44 ProfileIPc should complete in < 1:50:49 @17:59:18.45 PCR.run 2:23:31.25 @19:22:10.14 PCR.off @19:22:12.68 PCR for IPc completed -- closed /var/log/esp/IPc18aug2117h1500ml.pcr @19:22:12.75 Cleaning PCR @19:22:12.76 AFV.select :carrierSolution @19:22:12.88 delay 2 seconds @19:22:14.91 AS.reconfigure ASconfig @19:22:14.96 AS.seek 0.5ml @19:22:25.31 delay 1 second @19:22:26.33 AFV.select :holdingCoil @19:22:26.43 ASV.dial :waste1 @19:22:26.82 ATV.dial :air3 @19:22:28.12 AS.reconfigure ASslow1 @19:22:28.18 AS.seek 0.51ml @19:22:30.51 delay 2 seconds @19:22:32.53 ATV.dial :bleach @19:22:33.15 AS.seek 0.81ml @19:23:33.75 delay 3 seconds @19:23:36.77 ATV.dial :air3 @19:23:37.34 delay 3 seconds @19:23:40.41 AS.seek 0.835ml @19:23:45.65 delay 3 seconds @19:23:48.67 ATV.dial :bleach @19:23:49.29 AS.seek 0.82ml @19:23:52.59 delay 3 seconds @19:23:55.61 ARV.dial :waste @19:23:56.25 delay 3 seconds @19:23:59.27 ATV.dial :ARV @19:24:00.18 AS.reconfigure ASconfig @19:24:00.24 AS.seek 0.47ml @19:24:20.67 AFV.select :carrierSolution @19:24:20.78 delay 2 seconds @19:24:22.81 AS.seek 1ml @19:24:53.59 delay 1 second @19:24:54.61 AFV.select :holdingCoil @19:24:54.71 delay 1 second @19:24:55.74 AS.reconfigure ASfast @19:24:55.79 AS.seek 0ml @19:25:23.15 AFV.select :carrierSolution @19:25:23.25 delay 2 seconds @19:25:25.29 AS.reconfigure ASconfig @19:25:25.37 AS.seek 1ml @19:26:23.14 delay 1 second @19:26:24.16 AFV.select :holdingCoil @19:26:24.26 delay 1 second @19:26:25.29 AS.reconfigure ASfast @19:26:25.34 AS.seek 0ml @19:26:52.67 AFV.select :carrierSolution @19:26:52.78 delay 2 seconds @19:26:54.81 AS.reconfigure ASconfig @19:26:54.86 AS.seek 1ml @19:27:52.64 delay 1 second @19:27:53.66 AFV.select :holdingCoil @19:27:53.76 delay 1 second @19:27:54.79 AS.reconfigure ASfast @19:27:54.84 AS.seek 0ml @19:28:22.17 ARV.dial between carrier and air1 @19:28:22.68 ATV.dial :air3 @19:28:23.98 AS.reconfigure ASslow1 @19:28:24.04 AS.seek 0.01ml @19:28:26.39 delay 2 seconds @19:28:28.41 ATV.dial :bleach @19:28:29.03 AS.seek 0.81ml @19:31:10.22 delay 3 seconds @19:31:13.24 ATV.dial :air3 @19:31:13.79 delay 3 seconds @19:31:16.86 AS.seek 0.835ml @19:31:22.26 delay 3 seconds @19:31:25.28 ATV.dial :bleach @19:31:25.90 AS.seek 0.82ml @19:31:29.23 delay 3 seconds @19:31:32.25 ATV.dial PCR @19:31:32.67 AS.reconfigure ASconfig @19:31:32.73 AS.seek :empty @19:32:20.22 PCR.program :bleachProfile @19:32:20.27 PCR program should complete in < 2:45 @19:32:20.28 PCR.run 10:00 @19:35:19.58 PCR.off @19:35:19.61 AFV.select :carrierSolution @19:35:19.73 delay 2 seconds @19:35:21.76 AS.seek 1ml @19:36:19.59 delay 1 second @19:36:20.61 AFV.select :holdingCoil @19:36:20.71 delay 1 second @19:36:21.74 AS.reconfigure ASfast @19:36:21.80 AS.seek 0ml @19:36:49.23 delay 3 seconds @19:36:52.25 AFV.select :carrierSolution @19:36:52.35 delay 2 seconds @19:36:54.38 AS.reconfigure ASconfig @19:36:54.43 AS.seek 1ml @19:37:52.19 delay 1 second @19:37:53.21 AFV.select :holdingCoil @19:37:53.31 delay 1 second @19:37:54.34 AS.reconfigure ASfast @19:37:54.39 AS.seek 0ml @19:38:21.75 delay 3 seconds @19:38:24.77 AFV.select :carrierSolution @19:38:24.87 delay 2 seconds @19:38:26.90 AS.reconfigure ASconfig @19:38:26.96 AS.seek 1ml @19:39:24.72 delay 1 second @19:39:25.74 AFV.select :holdingCoil @19:39:25.84 delay 1 second @19:39:26.87 AS.reconfigure ASfast @19:39:26.92 AS.seek 0ml @19:39:54.28 delay 3 seconds @19:39:57.30 AFV.select :carrierSolution @19:39:57.40 delay 2 seconds @19:39:59.43 AS.reconfigure ASconfig @19:39:59.48 AS.seek 1ml @19:40:57.23 delay 1 second @19:40:58.25 AFV.select :holdingCoil @19:40:58.35 delay 1 second @19:40:59.38 AS.reconfigure ASfast @19:40:59.43 AS.seek 0ml @19:41:26.80 delay 3 seconds @19:41:29.82 AFV.select :carrierSolution @19:41:29.92 delay 2 seconds @19:41:31.96 AS.reconfigure ASconfig @19:41:32.03 AS.seek 1ml @19:42:29.78 delay 1 second @19:42:30.80 AFV.select :holdingCoil @19:42:30.90 delay 1 second @19:42:31.93 AS.reconfigure ASnorm @19:42:31.98 AS.seek 0ml @19:43:22.69 Setting up PCR for Rp -- writing /var/log/esp/Rp18aug2119h1500ml.pcr @19:43:22.70 Building PCR Slug from ASV with MM2 mix, PP3 primer @19:43:22.73 Priming with carrier @19:43:22.75 ATV.dial ARV @19:43:23.37 ARV.dial :carrier @19:43:23.73 AS.seek 0.5ml @19:43:49.31 AFV.select :carrierSolution @19:43:49.43 delay 2 seconds @19:43:51.46 AS.reconfigure ASconfig @19:43:51.52 AS.seek 1ml @19:44:20.56 delay 1 second @19:44:21.58 AFV.select :holdingCoil @19:44:21.68 delay 1 second @19:44:22.70 ATV.dial :waste @19:44:24.38 AS.reconfigure ASnorm @19:44:24.44 AS.seek :empty @19:45:15.20 AFV.select :carrierSolution @19:45:15.31 delay 2 seconds @19:45:17.34 AS.reconfigure ASconfig @19:45:17.40 AS.seek 0.5ml @19:45:46.48 delay 1 second @19:45:47.50 AFV.select :holdingCoil @19:45:47.60 Building Slug @19:45:47.61 ATV.dial :ARV @19:45:49.27 delay 2 seconds @19:45:51.29 ARV.dial :air1 @19:45:51.70 delay 2 seconds @19:45:53.77 AS.reconfigure ASslow2 @19:45:53.83 AS.seek 0.506ml @19:46:00.22 delay 2 seconds @19:46:02.24 ARV.dial :MM2 @19:46:03.77 delay 2 seconds @19:46:05.79 Adding MM2 @19:46:05.87 AS.seek 0.524ml @19:46:24.45 delay 25 seconds @19:46:49.47 ARV.dial :PP3 @19:46:49.88 Adding PP3 @19:46:49.89 delay 2 seconds @19:46:51.97 AS.seek 0.53ml @19:46:58.31 delay 10 seconds @19:47:08.34 ARV.dial :air2 @19:47:08.75 adding ARV air @19:47:08.76 delay 2 seconds @19:47:10.84 AS.seek 0.536ml @19:47:17.28 delay 5 seconds @19:47:22.30 ARV.dial :carrier @19:47:23.59 delay 7 seconds @19:47:30.67 AS.seek 0.594ml @19:48:29.34 delay 5 seconds @19:48:34.36 ATV.dial :ASV @19:48:35.27 delay 2 seconds @19:48:37.34 AS.seek 0.6ml @19:48:43.56 delay 4 seconds @19:48:47.58 ATV.dial ARV @19:48:48.13 delay 2 seconds @19:48:50.15 ARV.dial :carrier @19:48:50.16 delay 2 seconds @19:48:52.24 AS.seek 0.65ml @19:49:42.70 delay 4 seconds @19:49:46.72 ATV.dial PCR @19:49:47.34 ASV.dial :waste1 @19:49:47.35 ARV.dial :air1 @19:49:47.78 Positioning Slug by 0.324ml @19:49:47.87 AS.seek 0.326ml @19:55:13.96 delay 5 seconds @19:55:18.98 ATV.dial ARV @19:55:19.77 ASV.dial :PCRlock @19:55:20.17 delay 5 seconds @19:55:25.19 Running Rp Profile60C300 thermocycling @19:55:25.21 PCR.program Profile60C300 @19:55:25.35 Profile60C300 should complete in < 1:45:21 @19:55:25.36 PCR.run 2:16:41.25 @21:16:07.00 PCR.off @21:16:09.03 PCR for Rp completed -- closed /var/log/esp/Rp18aug2119h1500ml.pcr @21:16:09.11 Cleaning PCR @21:16:09.12 AFV.select :carrierSolution @21:16:09.24 delay 2 seconds @21:16:11.27 AS.reconfigure ASconfig @21:16:11.32 AS.seek 0.5ml @21:16:21.61 delay 1 second @21:16:22.63 AFV.select :holdingCoil @21:16:22.73 ASV.dial :waste1 @21:16:23.11 ATV.dial :air3 @21:16:24.41 AS.reconfigure ASslow1 @21:16:24.47 AS.seek 0.51ml @21:16:26.76 delay 2 seconds @21:16:28.78 ATV.dial :bleach @21:16:29.41 AS.seek 0.81ml @21:17:30.00 delay 3 seconds @21:17:33.02 ATV.dial :air3 @21:17:33.58 delay 3 seconds @21:17:36.65 AS.seek 0.835ml @21:17:41.89 delay 3 seconds @21:17:44.91 ATV.dial :bleach @21:17:45.53 AS.seek 0.82ml @21:17:48.85 delay 3 seconds @21:17:51.87 ARV.dial :waste @21:17:52.49 delay 3 seconds @21:17:55.51 ATV.dial :ARV @21:17:56.41 AS.reconfigure ASconfig @21:17:56.47 AS.seek 0.47ml @21:18:16.88 AFV.select :carrierSolution @21:18:16.99 delay 2 seconds @21:18:19.02 AS.seek 1ml @21:18:49.77 delay 1 second @21:18:50.79 AFV.select :holdingCoil @21:18:50.89 delay 1 second @21:18:51.92 AS.reconfigure ASfast @21:18:51.97 AS.seek 0ml @21:19:19.38 AFV.select :carrierSolution @21:19:19.49 delay 2 seconds @21:19:21.52 AS.reconfigure ASconfig @21:19:21.57 AS.seek 1ml @21:20:19.36 delay 1 second @21:20:20.38 AFV.select :holdingCoil @21:20:20.48 delay 1 second @21:20:21.51 AS.reconfigure ASfast @21:20:21.56 AS.seek 0ml @21:20:48.97 AFV.select :carrierSolution @21:20:49.08 delay 2 seconds @21:20:51.11 AS.reconfigure ASconfig @21:20:51.16 AS.seek 1ml @21:21:48.93 delay 1 second @21:21:49.95 AFV.select :holdingCoil @21:21:50.05 delay 1 second @21:21:51.08 AS.reconfigure ASfast @21:21:51.13 AS.seek 0ml @21:22:18.47 ARV.dial between carrier and air1 @21:22:18.99 ATV.dial :air3 @21:22:20.28 AS.reconfigure ASslow1 @21:22:20.34 AS.seek 0.01ml @21:22:22.66 delay 2 seconds @21:22:24.68 ATV.dial :bleach @21:22:25.30 AS.seek 0.81ml @21:25:06.47 delay 3 seconds @21:25:09.49 ATV.dial :air3 @21:25:10.05 delay 3 seconds @21:25:13.12 AS.seek 0.835ml @21:25:18.47 delay 3 seconds @21:25:21.49 ATV.dial :bleach @21:25:22.11 AS.seek 0.82ml @21:25:25.43 delay 3 seconds @21:25:28.45 ATV.dial PCR @21:25:28.86 AS.reconfigure ASconfig @21:25:28.92 AS.seek :empty @21:26:16.40 PCR.program :bleachProfile @21:26:16.46 PCR program should complete in < 2:45 @21:26:16.47 PCR.run 10:00 @21:29:18.30 PCR.off @21:29:18.33 AFV.select :carrierSolution @21:29:18.45 delay 2 seconds @21:29:20.48 AS.seek 1ml @21:30:18.29 delay 1 second @21:30:19.31 AFV.select :holdingCoil @21:30:19.41 delay 1 second @21:30:20.44 AS.reconfigure ASfast @21:30:20.49 AS.seek 0ml @21:30:48.57 delay 3 seconds @21:30:51.59 AFV.select :carrierSolution @21:30:51.69 delay 2 seconds @21:30:53.72 AS.reconfigure ASconfig @21:30:53.79 AS.seek 1ml @21:31:51.57 delay 1 second @21:31:52.59 AFV.select :holdingCoil @21:31:52.69 delay 1 second @21:31:53.72 AS.reconfigure ASfast @21:31:53.78 AS.seek 0ml @21:32:21.32 delay 3 seconds @21:32:24.34 AFV.select :carrierSolution @21:32:24.44 delay 2 seconds @21:32:26.47 AS.reconfigure ASconfig @21:32:26.53 AS.seek 1ml @21:33:24.32 delay 1 second @21:33:25.34 AFV.select :holdingCoil @21:33:25.44 delay 1 second @21:33:26.47 AS.reconfigure ASfast @21:33:26.52 AS.seek 0ml @21:33:53.88 delay 3 seconds @21:33:56.90 AFV.select :carrierSolution @21:33:57.00 delay 2 seconds @21:33:59.03 AS.reconfigure ASconfig @21:33:59.09 AS.seek 1ml @21:34:56.90 delay 1 second @21:34:57.92 AFV.select :holdingCoil @21:34:58.02 delay 1 second @21:34:59.05 AS.reconfigure ASfast @21:34:59.10 AS.seek 0ml @21:35:26.46 delay 3 seconds @21:35:29.48 AFV.select :carrierSolution @21:35:29.58 delay 2 seconds @21:35:31.61 AS.reconfigure ASconfig @21:35:31.67 AS.seek 1ml @21:36:29.44 delay 1 second @21:36:30.46 AFV.select :holdingCoil @21:36:30.56 delay 1 second @21:36:31.59 AS.reconfigure ASnorm @21:36:31.64 AS.seek 0ml @21:37:22.39 Setting up PCR for Ss -- writing /var/log/esp/Ss18aug2121h1500ml.pcr @21:37:22.40 Building PCR Slug from ASV with MM1 mix, PP2 primer @21:37:22.43 Priming with carrier @21:37:22.45 ATV.dial ARV @21:37:23.09 ARV.dial :carrier @21:37:23.44 AS.seek 0.5ml @21:37:49.02 AFV.select :carrierSolution @21:37:49.14 delay 2 seconds @21:37:51.17 AS.reconfigure ASconfig @21:37:51.23 AS.seek 1ml @21:38:20.30 delay 1 second @21:38:21.32 AFV.select :holdingCoil @21:38:21.42 delay 1 second @21:38:22.44 ATV.dial :waste @21:38:24.10 AS.reconfigure ASnorm @21:38:24.17 AS.seek :empty @21:39:14.91 AFV.select :carrierSolution @21:39:15.03 delay 2 seconds @21:39:17.06 AS.reconfigure ASconfig @21:39:17.12 AS.seek 0.5ml @21:39:46.26 delay 1 second @21:39:47.28 AFV.select :holdingCoil @21:39:47.38 Building Slug @21:39:47.39 ATV.dial :ARV @21:39:49.04 delay 2 seconds @21:39:51.06 ARV.dial :air1 @21:39:51.46 delay 2 seconds @21:39:53.53 AS.reconfigure ASslow2 @21:39:53.59 AS.seek 0.506ml @21:39:59.82 delay 2 seconds @21:40:01.84 ARV.dial :MM1 @21:40:02.45 delay 2 seconds @21:40:04.47 Adding MM1 @21:40:04.55 AS.seek 0.524ml @21:40:22.80 delay 25 seconds @21:40:47.82 ARV.dial :PP2 @21:40:48.23 Adding PP2 @21:40:48.24 delay 2 seconds @21:40:50.32 AS.seek 0.53ml @21:40:56.60 delay 10 seconds @21:41:06.63 ARV.dial :air2 @21:41:07.04 adding ARV air @21:41:07.05 delay 2 seconds @21:41:09.13 AS.seek 0.536ml @21:41:15.40 delay 5 seconds @21:41:20.42 ARV.dial :carrier @21:41:21.71 delay 7 seconds @21:41:28.79 AS.seek 0.594ml @21:42:27.30 delay 5 seconds @21:42:32.32 ATV.dial :ASV @21:42:32.71 delay 2 seconds @21:42:34.78 AS.seek 0.6ml @21:42:40.98 delay 4 seconds @21:42:45.00 ATV.dial ARV @21:42:45.56 delay 2 seconds @21:42:47.58 ARV.dial :carrier @21:42:47.59 delay 2 seconds @21:42:49.67 AS.seek 0.65ml @21:43:40.21 delay 4 seconds @21:43:44.23 ATV.dial PCR @21:43:44.85 ASV.dial :waste1 @21:43:44.86 ARV.dial :air1 @21:43:45.30 Positioning Slug by 0.324ml @21:43:45.39 AS.seek 0.326ml @21:49:12.06 delay 5 seconds @21:49:17.08 ATV.dial ARV @21:49:17.96 ASV.dial :PCRlock @21:49:18.35 delay 5 seconds @21:49:23.37 Running Ss Profile60C350 thermocycling @21:49:23.39 PCR.program Profile60C350 @21:49:23.54 Profile60C350 should complete in < 1:48:05 @21:49:23.55 PCR.run 2:20:06.25 @23:10:34.38 PCR.off @23:10:36.86 PCR for Ss completed -- closed /var/log/esp/Ss18aug2121h1500ml.pcr @23:10:36.94 Cleaning PCR @23:10:36.95 AFV.select :carrierSolution @23:10:37.07 delay 2 seconds @23:10:39.10 AS.reconfigure ASconfig @23:10:39.15 AS.seek 0.5ml @23:10:49.50 delay 1 second @23:10:50.52 AFV.select :holdingCoil @23:10:50.62 ASV.dial :waste1 @23:10:51.00 ATV.dial :air3 @23:10:52.27 AS.reconfigure ASslow1 @23:10:52.33 AS.seek 0.51ml @23:10:54.64 delay 2 seconds @23:10:56.66 ATV.dial :bleach @23:10:57.28 AS.seek 0.81ml @23:11:57.88 delay 3 seconds @23:12:00.90 ATV.dial :air3 @23:12:01.46 delay 3 seconds @23:12:04.53 AS.seek 0.835ml @23:12:09.77 delay 3 seconds @23:12:12.79 ATV.dial :bleach @23:12:13.41 AS.seek 0.82ml @23:12:16.71 delay 3 seconds @23:12:19.73 ARV.dial :waste @23:12:20.35 delay 3 seconds @23:12:23.37 ATV.dial :ARV @23:12:24.27 AS.reconfigure ASconfig @23:12:24.33 AS.seek 0.47ml @23:12:44.74 AFV.select :carrierSolution @23:12:44.85 delay 2 seconds @23:12:46.88 AS.seek 1ml @23:13:17.61 delay 1 second @23:13:18.63 AFV.select :holdingCoil @23:13:18.73 delay 1 second @23:13:19.76 AS.reconfigure ASfast @23:13:19.81 AS.seek 0ml @23:13:47.19 AFV.select :carrierSolution @23:13:47.30 delay 2 seconds @23:13:49.33 AS.reconfigure ASconfig @23:13:49.38 AS.seek 1ml @23:14:47.13 delay 1 second @23:14:48.15 AFV.select :holdingCoil @23:14:48.25 delay 1 second @23:14:49.28 AS.reconfigure ASfast @23:14:49.33 AS.seek 0ml @23:15:16.69 AFV.select :carrierSolution @23:15:16.80 delay 2 seconds @23:15:18.83 AS.reconfigure ASconfig @23:15:18.88 AS.seek 1ml @23:16:16.68 delay 1 second @23:16:17.70 AFV.select :holdingCoil @23:16:17.80 delay 1 second @23:16:18.83 AS.reconfigure ASfast @23:16:18.88 AS.seek 0ml @23:16:46.24 ARV.dial between carrier and air1 @23:16:46.74 ATV.dial :air3 @23:16:48.03 AS.reconfigure ASslow1 @23:16:48.09 AS.seek 0.01ml @23:16:50.38 delay 2 seconds @23:16:52.40 ATV.dial :bleach @23:16:53.02 AS.seek 0.81ml @23:19:34.16 delay 3 seconds @23:19:37.18 ATV.dial :air3 @23:19:37.74 delay 3 seconds @23:19:40.81 AS.seek 0.835ml @23:19:46.13 delay 3 seconds @23:19:49.15 ATV.dial :bleach @23:19:49.77 AS.seek 0.82ml @23:19:53.08 delay 3 seconds @23:19:56.10 ATV.dial PCR @23:19:56.52 AS.reconfigure ASconfig @23:19:56.58 AS.seek :empty @23:20:44.05 PCR.program :bleachProfile @23:20:44.10 PCR program should complete in < 2:45 @23:20:44.11 PCR.run 10:00 @23:23:42.92 PCR.off @23:23:42.95 AFV.select :carrierSolution @23:23:43.07 delay 2 seconds @23:23:45.10 AS.seek 1ml @23:24:42.90 delay 1 second @23:24:43.92 AFV.select :holdingCoil @23:24:44.02 delay 1 second @23:24:45.05 AS.reconfigure ASfast @23:24:45.10 AS.seek 0ml @23:25:12.57 delay 3 seconds @23:25:15.59 AFV.select :carrierSolution @23:25:15.69 delay 2 seconds @23:25:17.72 AS.reconfigure ASconfig @23:25:17.78 AS.seek 1ml @23:26:15.59 delay 1 second @23:26:17.11 AFV.select :holdingCoil @23:26:17.23 delay 1 second @23:26:18.26 AS.reconfigure ASfast @23:26:18.31 AS.seek 0ml @23:26:45.79 delay 3 seconds @23:26:48.81 AFV.select :carrierSolution @23:26:48.91 delay 2 seconds @23:26:50.94 AS.reconfigure ASconfig @23:26:51.00 AS.seek 1ml @23:27:48.76 delay 1 second @23:27:49.78 AFV.select :holdingCoil @23:27:49.88 delay 1 second @23:27:50.91 AS.reconfigure ASfast @23:27:50.96 AS.seek 0ml @23:28:18.32 delay 3 seconds @23:28:21.34 AFV.select :carrierSolution @23:28:21.44 delay 2 seconds @23:28:23.47 AS.reconfigure ASconfig @23:28:23.53 AS.seek 1ml @23:29:21.30 delay 1 second @23:29:22.32 AFV.select :holdingCoil @23:29:22.42 delay 1 second @23:29:23.45 AS.reconfigure ASfast @23:29:23.50 AS.seek 0ml @23:29:50.87 delay 3 seconds @23:29:53.89 AFV.select :carrierSolution @23:29:53.99 delay 2 seconds @23:29:56.02 AS.reconfigure ASconfig @23:29:56.08 AS.seek 1ml @23:30:53.82 delay 1 second @23:30:54.84 AFV.select :holdingCoil @23:30:54.95 delay 1 second @23:30:55.98 AS.reconfigure ASnorm @23:30:56.03 AS.seek 0ml @23:31:46.73 Setting up PCR for Bb -- writing /var/log/esp/Bb18aug2123h1500ml.pcr @23:31:46.74 Building PCR Slug from ASV with MM3 mix, PP5 primer @23:31:46.77 Priming with carrier @23:31:46.79 ATV.dial ARV @23:31:47.52 ARV.dial :carrier @23:31:47.88 AS.seek 0.5ml @23:32:13.46 AFV.select :carrierSolution @23:32:13.58 delay 2 seconds @23:32:15.61 AS.reconfigure ASconfig @23:32:15.67 AS.seek 1ml @23:32:44.71 delay 1 second @23:32:45.73 AFV.select :holdingCoil @23:32:45.83 delay 1 second @23:32:46.85 ATV.dial :waste @23:32:48.49 AS.reconfigure ASnorm @23:32:48.56 AS.seek :empty @23:33:39.34 AFV.select :carrierSolution @23:33:39.45 delay 2 seconds @23:33:41.48 AS.reconfigure ASconfig @23:33:41.54 AS.seek 0.5ml @23:34:10.68 delay 1 second @23:34:11.70 AFV.select :holdingCoil @23:34:11.80 Building Slug @23:34:11.81 ATV.dial :ARV @23:34:13.46 delay 2 seconds @23:34:15.48 ARV.dial :air1 @23:34:15.88 delay 2 seconds @23:34:17.95 AS.reconfigure ASslow2 @23:34:18.02 AS.seek 0.506ml @23:34:24.38 delay 2 seconds @23:34:26.40 ARV.dial :MM3 @23:34:27.48 delay 2 seconds @23:34:29.50 Adding MM3 @23:34:29.59 AS.seek 0.524ml @23:34:47.90 delay 25 seconds @23:35:12.92 ARV.dial :PP5 @23:35:13.32 Adding PP5 @23:35:13.33 delay 2 seconds @23:35:15.41 AS.seek 0.53ml @23:35:21.70 delay 10 seconds @23:35:31.73 ARV.dial :air3 @23:35:32.12 adding ARV air @23:35:32.13 delay 2 seconds @23:35:34.21 AS.seek 0.536ml @23:35:40.53 delay 5 seconds @23:35:45.55 ARV.dial :carrier @23:35:46.84 delay 7 seconds @23:35:53.92 AS.seek 0.594ml @23:36:52.49 delay 5 seconds @23:36:57.51 ATV.dial :ASV @23:36:57.93 delay 2 seconds @23:37:00.00 AS.seek 0.6ml @23:37:06.29 delay 4 seconds @23:37:10.31 ATV.dial ARV @23:37:10.87 delay 2 seconds @23:37:12.89 ARV.dial :carrier @23:37:12.90 delay 2 seconds @23:37:14.98 AS.seek 0.65ml @23:38:05.67 delay 4 seconds @23:38:09.69 ATV.dial PCR @23:38:10.32 ASV.dial :waste1 @23:38:10.33 ARV.dial :air1 @23:38:10.75 Positioning Slug by 0.324ml @23:38:10.85 AS.seek 0.326ml @23:43:36.98 delay 5 seconds @23:43:42.00 ATV.dial ARV @23:43:42.79 ASV.dial :PCRlock @23:43:43.68 delay 5 seconds @23:43:48.70 Running Bb Profile60C300 thermocycling @23:43:48.72 PCR.program Profile60C300 @23:43:48.86 Profile60C300 should complete in < 1:45:21 @23:43:48.87 PCR.run 2:16:41.25 @01:04:42.41CEST22-Aug-18 PCR.off @01:04:43.93 PCR for Bb completed -- closed /var/log/esp/Bb18aug2123h1500ml.pcr @01:04:44.01 Cleaning PCR @01:04:44.02 AFV.select :carrierSolution @01:04:44.14 delay 2 seconds @01:04:46.17 AS.reconfigure ASconfig @01:04:46.22 AS.seek 0.5ml @01:04:56.54 delay 1 second @01:04:57.56 AFV.select :holdingCoil @01:04:57.66 ASV.dial :waste1 @01:04:58.04 ATV.dial :air3 @01:04:59.34 AS.reconfigure ASslow1 @01:04:59.40 AS.seek 0.51ml @01:05:01.71 delay 2 seconds @01:05:03.73 ATV.dial :bleach @01:05:04.35 AS.seek 0.81ml @01:06:04.95 delay 3 seconds @01:06:07.97 ATV.dial :air3 @01:06:09.04 delay 3 seconds @01:06:12.11 AS.seek 0.835ml @01:06:17.48 delay 3 seconds @01:06:20.50 ATV.dial :bleach @01:06:21.12 AS.seek 0.82ml @01:06:24.43 delay 3 seconds @01:06:27.45 ARV.dial :waste @01:06:28.07 delay 3 seconds @01:06:31.09 ATV.dial :ARV @01:06:31.99 AS.reconfigure ASconfig @01:06:32.05 AS.seek 0.47ml @01:06:52.47 AFV.select :carrierSolution @01:06:52.58 delay 2 seconds @01:06:54.61 AS.seek 1ml @01:07:25.36 delay 1 second @01:07:26.38 AFV.select :holdingCoil @01:07:26.48 delay 1 second @01:07:27.51 AS.reconfigure ASfast @01:07:27.56 AS.seek 0ml @01:07:54.92 AFV.select :carrierSolution @01:07:55.04 delay 2 seconds @01:07:57.07 AS.reconfigure ASconfig @01:07:57.12 AS.seek 1ml @01:08:54.89 delay 1 second @01:08:55.91 AFV.select :holdingCoil @01:08:56.01 delay 1 second @01:08:57.04 AS.reconfigure ASfast @01:08:57.09 AS.seek 0ml @01:09:24.45 AFV.select :carrierSolution @01:09:24.56 delay 2 seconds @01:09:26.59 AS.reconfigure ASconfig @01:09:26.64 AS.seek 1ml @01:10:24.39 delay 1 second @01:10:25.41 AFV.select :holdingCoil @01:10:25.51 delay 1 second @01:10:26.54 AS.reconfigure ASfast @01:10:26.59 AS.seek 0ml @01:10:53.95 ARV.dial between carrier and air1 @01:10:54.45 ATV.dial :air3 @01:10:55.75 AS.reconfigure ASslow1 @01:10:55.81 AS.seek 0.01ml @01:10:58.12 delay 2 seconds @01:11:00.14 ATV.dial :bleach @01:11:00.76 AS.seek 0.81ml @01:13:41.95 delay 3 seconds @01:13:44.97 ATV.dial :air3 @01:13:45.53 delay 3 seconds @01:13:48.60 AS.seek 0.835ml @01:13:53.90 delay 3 seconds @01:13:56.92 ATV.dial :bleach @01:13:57.53 AS.seek 0.82ml @01:14:00.84 delay 3 seconds @01:14:03.86 ATV.dial PCR @01:14:04.28 AS.reconfigure ASconfig @01:14:04.34 AS.seek :empty @01:14:51.81 PCR.program :bleachProfile @01:14:51.86 PCR program should complete in < 2:45 @01:14:51.87 PCR.run 10:00 @01:17:50.67 PCR.off @01:17:50.71 AFV.select :carrierSolution @01:17:50.82 delay 2 seconds @01:17:52.85 AS.seek 1ml @01:18:50.69 delay 1 second @01:18:51.71 AFV.select :holdingCoil @01:18:51.81 delay 1 second @01:18:52.84 AS.reconfigure ASfast @01:18:52.89 AS.seek 0ml @01:19:20.23 delay 3 seconds @01:19:23.25 AFV.select :carrierSolution @01:19:23.35 delay 2 seconds @01:19:25.38 AS.reconfigure ASconfig @01:19:25.43 AS.seek 1ml @01:20:23.19 delay 1 second @01:20:24.21 AFV.select :holdingCoil @01:20:24.31 delay 1 second @01:20:25.34 AS.reconfigure ASfast @01:20:25.39 AS.seek 0ml @01:20:52.89 delay 3 seconds @01:20:55.91 AFV.select :carrierSolution @01:20:56.01 delay 2 seconds @01:20:58.04 AS.reconfigure ASconfig @01:20:58.10 AS.seek 1ml @01:21:55.84 delay 1 second @01:21:56.86 AFV.select :holdingCoil @01:21:56.96 delay 1 second @01:21:57.99 AS.reconfigure ASfast @01:21:58.04 AS.seek 0ml @01:22:25.41 delay 3 seconds @01:22:28.43 AFV.select :carrierSolution @01:22:28.53 delay 2 seconds @01:22:30.56 AS.reconfigure ASconfig @01:22:30.62 AS.seek 1ml @01:23:28.37 delay 1 second @01:23:29.39 AFV.select :holdingCoil @01:23:29.49 delay 1 second @01:23:30.52 AS.reconfigure ASfast @01:23:30.57 AS.seek 0ml @01:23:58.45 delay 3 seconds @01:24:01.48 AFV.select :carrierSolution @01:24:01.58 delay 2 seconds @01:24:03.61 AS.reconfigure ASconfig @01:24:03.68 AS.seek 1ml @01:25:01.44 delay 1 second @01:25:02.46 AFV.select :holdingCoil @01:25:02.56 delay 1 second @01:25:03.59 AS.reconfigure ASnorm @01:25:03.64 AS.seek 0ml @01:25:54.36 Setting up PCR for Tt2 -- writing /var/log/esp/Tt218aug2201h1500ml.pcr @01:25:54.37 Building PCR Slug from ASV with MM3 mix, PP6 primer @01:25:54.39 Priming with carrier @01:25:54.42 ATV.dial ARV @01:25:55.03 ARV.dial :carrier @01:25:55.39 AS.seek 0.5ml @01:26:20.94 AFV.select :carrierSolution @01:26:21.06 delay 2 seconds @01:26:23.09 AS.reconfigure ASconfig @01:26:23.15 AS.seek 1ml @01:26:52.17 delay 1 second @01:26:53.19 AFV.select :holdingCoil @01:26:53.29 delay 1 second @01:26:54.31 ATV.dial :waste @01:26:55.97 AS.reconfigure ASnorm @01:26:56.04 AS.seek :empty @01:27:46.77 AFV.select :carrierSolution @01:27:46.89 delay 2 seconds @01:27:48.92 AS.reconfigure ASconfig @01:27:48.98 AS.seek 0.5ml @01:28:18.06 delay 1 second @01:28:19.08 AFV.select :holdingCoil @01:28:19.18 Building Slug @01:28:19.19 ATV.dial :ARV @01:28:20.84 delay 2 seconds @01:28:22.86 ARV.dial :air1 @01:28:23.28 delay 2 seconds @01:28:25.35 AS.reconfigure ASslow2 @01:28:25.42 AS.seek 0.506ml @01:28:31.91 delay 2 seconds @01:28:33.93 ARV.dial :MM3 @01:28:34.99 delay 2 seconds @01:28:37.01 Adding MM3 @01:28:37.10 AS.seek 0.524ml @01:28:55.55 delay 25 seconds @01:29:20.57 ARV.dial :PP6 @01:29:20.95 Adding PP6 @01:29:20.96 delay 2 seconds @01:29:23.04 AS.seek 0.53ml @01:29:29.22 delay 10 seconds @01:29:39.25 ARV.dial :air1 @01:29:40.09 adding ARV air @01:29:40.10 delay 2 seconds @01:29:42.18 AS.seek 0.536ml @01:29:48.42 delay 5 seconds @01:29:53.44 ARV.dial :carrier @01:29:53.86 delay 7 seconds @01:30:00.94 AS.seek 0.594ml @01:30:59.47 delay 5 seconds @01:31:04.49 ATV.dial :ASV @01:31:04.91 delay 2 seconds @01:31:06.98 AS.seek 0.6ml @01:31:13.28 delay 4 seconds @01:31:17.30 ATV.dial ARV @01:31:17.86 delay 2 seconds @01:31:19.88 ARV.dial :carrier @01:31:19.89 delay 2 seconds @01:31:21.97 AS.seek 0.65ml @01:32:12.60 delay 4 seconds @01:32:16.62 ATV.dial PCR @01:32:17.25 ASV.dial :waste1 @01:32:17.26 ARV.dial :air1 @01:32:17.70 Positioning Slug by 0.324ml @01:32:17.79 AS.seek 0.326ml @01:37:43.92 delay 5 seconds @01:37:48.94 ATV.dial ARV @01:37:49.72 ASV.dial :PCRlock @01:37:50.11 delay 5 seconds @01:37:55.13 Running Tt2 Profile60C300 thermocycling @01:37:55.14 PCR.program Profile60C300 @01:37:55.29 Profile60C300 should complete in < 1:45:21 @01:37:55.30 PCR.run 2:16:41.25 @02:58:32.92 PCR.off @02:58:34.44 PCR for Tt2 completed -- closed /var/log/esp/Tt218aug2201h1500ml.pcr @02:58:34.45 Full MFB Decontamination @02:58:34.48 Decon extraction column and decon AIV @02:58:34.51 SPE.hold 2:46:40,55C @02:58:34.57 ASV.dial :SPEdelivery @02:58:35.24 delay 20 seconds @02:58:55.27 AFV.select :carrierSolution @02:58:55.37 delay 2 seconds @02:58:57.40 AS.reconfigure ASconfig @02:58:57.45 AS.seek 0.5ml @02:59:07.75 delay 1 second @02:59:08.77 AFV.select :holdingCoil @02:59:08.87 ATV.dial :air1 @02:59:10.86 AS.reconfigure ASslow1 @02:59:10.92 AS.seek 0.52ml @02:59:15.13 ATV.dial :ASV @02:59:16.96 ASV.dial :SPErecovery @02:59:17.64 AS.seek 0.62ml @02:59:38.04 ATV.dial :waste @02:59:40.11 AS.reconfigure ASnorm @02:59:40.17 AS.seek :empty @03:00:11.79 AFV.select :holdingCoil @03:00:11.80 ATV.dial :waste @03:00:11.83 AS.reconfigure ASconfig @03:00:11.90 AS.seek 0ml @03:00:12.21 AFV.select :carrierSolution @03:00:12.32 delay 2 seconds @03:00:14.35 AS.seek 0.5ml @03:00:43.38 delay 1 second @03:00:44.40 AFV.select :holdingCoil @03:00:44.50 ATV.dial :air3 @03:00:45.22 AS.reconfigure ASslow1 @03:00:45.28 AS.seek 0.52ml @03:00:50.11 ATV.dial :air3 @03:00:50.20 AS.seek 0.53ml @03:00:52.41 delay 2 seconds @03:00:54.43 ATV.dial :bleach @03:00:54.92 AS.seek 0.71ml @03:01:31.41 delay 3 seconds @03:01:34.43 ATV.dial :air3 @03:01:35.04 delay 3 seconds @03:01:38.11 AS.seek 0.735ml @03:01:43.33 delay 3 seconds @03:01:46.35 ATV.dial :bleach @03:01:47.00 AS.seek 0.72ml @03:01:50.33 delay 3 seconds @03:01:53.35 ATV.dial ASV @03:01:54.46 ASV.dial :SPErecovery @03:01:54.55 AS.seek 0.365ml @03:03:06.21 ATV.dial :waste @03:03:08.00 AS.reconfigure ASnorm @03:03:08.07 AS.seek :empty @03:03:26.86 ATV.dial :air3 @03:03:27.56 AS.reconfigure ASslow1 @03:03:27.62 AS.seek 0.009ml @03:03:30.00 delay 2 seconds @03:03:32.02 ATV.dial :bleach @03:03:32.50 AS.seek 0.579ml @03:05:27.36 delay 3 seconds @03:05:30.38 ATV.dial :air3 @03:05:30.94 delay 3 seconds @03:05:34.01 AS.seek 0.604ml @03:05:39.33 delay 3 seconds @03:05:42.35 ATV.dial :bleach @03:05:42.97 AS.seek 0.589ml @03:05:46.29 delay 3 seconds @03:05:49.31 ATV.dial :ASV @03:05:50.35 ASV.dial :wasteR @03:05:50.75 AS.seek 0ml @03:07:49.52 delay 1:00 @03:08:49.55 ATV.dial ASV @03:08:49.56 AFV.select :carrierSolution @03:08:49.68 delay 2 seconds @03:08:51.71 AS.reconfigure ASconfig @03:08:51.76 AS.seek 1ml @03:09:49.55 delay 1 second @03:09:50.57 AFV.select :holdingCoil @03:09:50.67 delay 1 second @03:09:51.69 ASV.dial :wasteR @03:09:51.71 AS.reconfigure ASslow1 @03:09:51.78 AS.seek 0ml @03:13:13.19 delay 10 seconds @03:13:23.22 ATV.dial :air3 @03:13:24.74 AS.seek 0.01ml @03:13:27.05 delay 2 seconds @03:13:29.07 ATV.dial :bleach @03:13:29.71 AS.seek 0.58ml @03:15:24.55 delay 3 seconds @03:15:27.57 ATV.dial :air3 @03:15:28.15 delay 3 seconds @03:15:31.22 AS.seek 0.605ml @03:15:36.52 delay 3 seconds @03:15:39.54 ATV.dial :bleach @03:15:40.18 AS.seek 0.59ml @03:15:43.43 delay 3 seconds @03:15:46.45 ATV.dial ASV @03:15:47.51 ASV.dial :SPEdelivery @03:15:47.89 AS.seek 0ml @03:17:46.84 delay 1:00 @03:18:46.87 AFV.select :carrierSolution @03:18:46.97 delay 2 seconds @03:18:49.00 AS.reconfigure ASconfig @03:18:49.05 AS.seek 1ml @03:19:46.84 delay 1 second @03:19:47.86 AFV.select :holdingCoil @03:19:47.96 delay 1 second @03:19:48.98 ATV.dial ASV @03:19:48.99 ASV.dial :SPEdelivery @03:19:49.02 AS.reconfigure ASslow1 @03:19:49.09 AS.seek 0ml @03:23:10.51 delay 10 seconds @03:23:20.54 ATV.dial :air2 @03:23:21.38 AS.reconfigure ASslow0 @03:23:21.44 AS.seek 0.8ml @03:24:42.18 delay 3 seconds @03:24:45.20 ATV.dial :ASV @03:24:46.20 ASV.dial :SPEdelivery @03:24:46.22 AS.reconfigure ASslow1 @03:24:46.29 AS.seek 0.03ml @03:27:21.43 delay 20 seconds @03:27:41.46 ATV.dial :waste,via: :air3 @03:27:43.82 AS.reconfigure ASconfig @03:27:43.88 AS.seek :empty @03:27:45.98 SPE.coast @03:27:46.06 Removing Bleach from Mixing Coil @03:27:46.07 AFV.select :carrierSolution @03:27:46.19 delay 2 seconds @03:27:48.22 AS.seek 0.5ml @03:28:17.29 delay 1 second @03:28:18.31 AFV.select :holdingCoil @03:28:18.41 ATV.dial :ASV @03:28:20.15 ASV.dial :SPErecovery @03:28:20.80 AS.reconfigure ASslow1 @03:28:20.86 AS.seek 0.73ml @03:29:07.40 ASV.dial :wasteD @03:29:08.07 AS.reconfigure ASnorm @03:29:08.14 AS.seek 0ml @03:29:45.28 AFV.select :carrierSolution @03:29:45.40 delay 2 seconds @03:29:47.43 AS.reconfigure ASconfig @03:29:47.48 AS.seek 0.5ml @03:30:16.54 delay 1 second @03:30:17.56 AFV.select :holdingCoil @03:30:17.66 ATV.dial ASV @03:30:17.68 ASV.dial :SPErecovery @03:30:18.34 AS.reconfigure ASslow0 @03:30:18.40 AS.seek 0.3ml @03:30:38.84 ATV.dial :air2 @03:30:39.88 AS.reconfigure ASslow1 @03:30:39.94 AS.seek 0.32ml @03:30:44.30 ATV.dial ASV @03:30:45.35 AS.reconfigure ASslow0 @03:30:45.41 AS.seek 0.57ml @03:31:10.90 ASV.dial :wasteD @03:31:11.49 AS.reconfigure ASnorm @03:31:11.56 AS.seek 0ml @03:31:40.64 AFV.select :carrierSolution @03:31:40.75 delay 2 seconds @03:31:42.78 AS.reconfigure ASconfig @03:31:42.83 AS.seek 0.5ml @03:32:11.92 delay 1 second @03:32:12.94 AFV.select :holdingCoil @03:32:13.04 ATV.dial ASV @03:32:13.06 ASV.dial :SPErecovery @03:32:13.71 AS.reconfigure ASslow0 @03:32:13.77 AS.seek 0.3ml @03:32:34.20 ATV.dial :air2 @03:32:35.24 AS.reconfigure ASslow1 @03:32:35.30 AS.seek 0.32ml @03:32:39.71 ATV.dial ASV @03:32:40.76 AS.reconfigure ASslow0 @03:32:40.82 AS.seek 0.57ml @03:33:06.21 ASV.dial :wasteD @03:33:06.81 AS.reconfigure ASnorm @03:33:06.87 AS.seek 0ml @03:33:35.95 ATV.dial :air2 @03:33:36.93 AS.reconfigure ASslow0 @03:33:36.99 AS.seek 0.3ml @03:34:07.57 ATV.dial :ASV @03:34:08.57 ASV.dial :wasteD @03:34:08.59 AS.reconfigure ASconfig @03:34:08.66 AS.seek 0.05ml @03:34:23.35 ATV.dial :waste @03:34:25.22 AS.seek 0ml @03:34:28.44 ASV.dial :SPEdelivery @03:34:29.43 deconLysateINT @03:34:29.44 ATV.dial :air3 @03:34:30.13 AS.reconfigure ASslow1 @03:34:30.19 AS.seek 0.01ml @03:34:32.56 delay 2 seconds @03:34:34.58 ATV.dial :bleach @03:34:35.04 AS.seek 0.96ml @03:37:46.40 delay 3 seconds @03:37:49.42 ATV.dial :air3 @03:37:49.98 delay 3 seconds @03:37:53.05 AS.seek 0.985ml @03:37:58.36 delay 3 seconds @03:38:01.38 ATV.dial :bleach @03:38:02.01 AS.seek 0.97ml @03:38:05.34 delay 3 seconds @03:38:08.36 ATV.dial AIV @03:38:09.62 AIV.dial :coilToMFB @03:38:09.63 AWV.select :waste @03:38:09.65 AFV.select :carrierSolution @03:38:09.78 delay 2 seconds @03:38:12.33 AS.reconfigure ASconfig @03:38:12.39 AS.seek 1ml @03:38:14.45 delay 1 second @03:38:15.47 AFV.select :holdingCoil @03:38:15.57 delay 1 second @03:38:16.60 AS.reconfigure ASslow0 @03:38:16.65 AS.seek :empty @03:39:57.61 AFV.select :carrierSolution @03:39:57.73 delay 2 seconds @03:39:59.76 AS.reconfigure ASconfig @03:39:59.82 AS.seek 1ml @03:40:57.67 delay 1 second @03:40:58.69 AFV.select :holdingCoil @03:40:58.79 delay 1 second @03:40:59.82 AS.reconfigure ASslow0 @03:40:59.87 AS.seek :empty @03:42:40.83 AFV.select :carrierSolution @03:42:40.95 delay 2 seconds @03:42:42.98 AS.reconfigure ASconfig @03:42:43.04 AS.seek 1ml @03:43:40.87 delay 1 second @03:43:41.89 AFV.select :holdingCoil @03:43:41.99 delay 1 second @03:43:43.02 AS.reconfigure ASslow0 @03:43:43.07 AS.seek :empty @03:45:24.04 ATV.dial :air2 @03:45:24.71 AS.reconfigure ASslow1 @03:45:24.77 AS.seek 0.009ml @03:45:27.09 AS.reconfigure ASslow0 @03:45:27.16 AS.seek 1ml @03:47:07.09 ATV.dial AIV @03:47:07.87 AS.seek 0.02ml @03:48:46.76 ATV.dial :waste @03:48:48.43 AS.seek 0ml @03:48:50.73 AWV.select :air @03:48:50.89 Cleaning PCR @03:48:50.90 AFV.select :carrierSolution @03:48:51.01 delay 2 seconds @03:48:53.04 AS.reconfigure ASconfig @03:48:53.09 AS.seek 0.5ml @03:49:22.17 delay 1 second @03:49:23.19 AFV.select :holdingCoil @03:49:23.29 ASV.dial :waste1 @03:49:23.69 ATV.dial :air3 @03:49:24.37 AS.reconfigure ASslow1 @03:49:24.43 AS.seek 0.51ml @03:49:26.72 delay 2 seconds @03:49:28.74 ATV.dial :bleach @03:49:29.21 AS.seek 0.81ml @03:50:29.83 delay 3 seconds @03:50:32.85 ATV.dial :air3 @03:50:33.42 delay 3 seconds @03:50:36.49 AS.seek 0.835ml @03:50:41.79 delay 3 seconds @03:50:44.81 ATV.dial :bleach @03:50:45.43 AS.seek 0.82ml @03:50:48.70 delay 3 seconds @03:50:51.72 ARV.dial :waste @03:50:52.34 delay 3 seconds @03:50:55.36 ATV.dial :ARV @03:50:56.23 AS.reconfigure ASconfig @03:50:56.29 AS.seek 0.47ml @03:51:16.72 AFV.select :carrierSolution @03:51:16.83 delay 2 seconds @03:51:18.86 AS.seek 1ml @03:51:49.64 delay 1 second @03:51:50.66 AFV.select :holdingCoil @03:51:50.76 delay 1 second @03:51:51.79 AS.reconfigure ASfast @03:51:51.84 AS.seek 0ml @03:52:19.20 AFV.select :carrierSolution @03:52:19.31 delay 2 seconds @03:52:21.34 AS.reconfigure ASconfig @03:52:21.39 AS.seek 1ml @03:53:19.16 delay 1 second @03:53:20.18 AFV.select :holdingCoil @03:53:20.28 delay 1 second @03:53:21.31 AS.reconfigure ASfast @03:53:21.36 AS.seek 0ml @03:53:48.70 AFV.select :carrierSolution @03:53:48.81 delay 2 seconds @03:53:50.84 AS.reconfigure ASconfig @03:53:50.89 AS.seek 1ml @03:54:48.66 delay 1 second @03:54:49.68 AFV.select :holdingCoil @03:54:49.78 delay 1 second @03:54:50.81 AS.reconfigure ASfast @03:54:50.86 AS.seek 0ml @03:55:18.22 ARV.dial between carrier and air1 @03:55:18.72 ATV.dial :air3 @03:55:20.01 AS.reconfigure ASslow1 @03:55:20.07 AS.seek 0.01ml @03:55:22.39 delay 2 seconds @03:55:24.41 ATV.dial :bleach @03:55:25.03 AS.seek 0.81ml @03:57:49.32 Session Ended @03:58:06.20 delay 3 seconds @03:58:09.22 ATV.dial :air3 @03:58:09.77 delay 3 seconds @03:58:13.35 AS.seek 0.835ml @03:58:18.65 delay 3 seconds @03:58:21.67 ATV.dial :bleach @03:58:22.28 AS.seek 0.82ml @03:58:25.53 delay 3 seconds @03:58:28.55 ATV.dial PCR @03:58:28.94 AS.reconfigure ASconfig @03:58:28.99 AS.seek :empty @03:59:16.42 PCR.program :bleachProfile @03:59:16.47 PCR program should complete in < 2:45 @03:59:16.48 PCR.run 10:00 @04:02:21.31 PCR.off @04:02:21.34 AFV.select :carrierSolution @04:02:21.44 delay 2 seconds @04:02:23.47 AS.seek 1ml @04:03:21.27 delay 1 second @04:03:22.29 AFV.select :holdingCoil @04:03:22.39 delay 1 second @04:03:23.42 AS.reconfigure ASfast @04:03:23.46 AS.seek 0ml @04:03:50.81 delay 3 seconds @04:03:53.83 AFV.select :carrierSolution @04:03:53.93 delay 2 seconds @04:03:55.96 AS.reconfigure ASconfig @04:03:56.00 AS.seek 1ml @04:04:53.74 delay 1 second @04:04:54.76 AFV.select :holdingCoil @04:04:54.86 delay 1 second @04:04:55.89 AS.reconfigure ASfast @04:04:55.93 AS.seek 0ml @04:05:23.38 delay 3 seconds @04:05:26.40 AFV.select :carrierSolution @04:05:26.50 delay 2 seconds @04:05:28.53 AS.reconfigure ASconfig @04:05:28.58 AS.seek 1ml @04:06:26.31 delay 1 second @04:06:27.33 AFV.select :holdingCoil @04:06:27.43 delay 1 second @04:06:28.46 AS.reconfigure ASfast @04:06:28.50 AS.seek 0ml @04:06:55.85 delay 3 seconds @04:06:58.87 AFV.select :carrierSolution @04:06:58.97 delay 2 seconds @04:07:01.00 AS.reconfigure ASconfig @04:07:01.05 AS.seek 1ml @04:07:58.80 delay 1 second @04:07:59.82 AFV.select :holdingCoil @04:07:59.92 delay 1 second @04:08:00.95 AS.reconfigure ASfast @04:08:00.99 AS.seek 0ml @04:08:28.35 delay 3 seconds @04:08:31.37 AFV.select :carrierSolution @04:08:31.47 delay 2 seconds @04:08:33.50 AS.reconfigure ASconfig @04:08:33.55 AS.seek 1ml @04:09:31.31 delay 1 second @04:09:32.33 AFV.select :holdingCoil @04:09:32.43 delay 1 second @04:09:33.46 AS.reconfigure ASnorm @04:09:33.50 AS.seek 0ml @04:10:24.20 AFV.select :carrierSolution @04:10:24.30 delay 2 seconds @04:10:26.33 AS.reconfigure ASconfig @04:10:26.37 AS.seek 1ml @04:11:24.16 delay 1 second @04:11:25.18 AFV.select :holdingCoil @04:11:25.28 delay 1 second @04:11:26.30 ATV.dial :waste @04:11:27.50 AS.reconfigure ASnorm @04:11:27.55 AS.seek 0.5ml @04:11:53.10 ATV.dial :waste2 @04:11:54.37 AS.seek 0ml @04:12:19.91
Asleep after completing 18tun1 assays @04:12:19.92 GoodNews.email "{1 => 3, 2 => 0}\nCan@04:11:32, 16.3C, 41% humidity, 14.7psia, 12.065V, 0.443A, 0.443A avg, 5.34W",Subject: "Asleep after completing 18tun1 assays" @04:12:19.94
Mission runtime was 15:00:48.70517 @10:29:09.32CEST23-Aug-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @10:29:11.02 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @10:29:11.35 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @10:29:16.14 Polling Can every 10:00 @10:29:18.30 SPE.coast @10:29:18.37 SPE.configure SPEconfig @10:29:18.45 AS.configure ASconfig @10:29:18.53 SS.reconfigure SSconfig @10:29:18.57 SS.hold @10:29:18.62 SS.configure SSconfig @10:29:18.71 CC.reconfigure CCconfig @10:29:18.75 CC.hold @10:29:18.81 CC.configure CCconfig @10:29:18.89 CS.reconfigure CSconfig @10:29:18.93 CS.hold @10:29:19.02 CS.configure CSconfig @10:29:19.19 PC.reconfigure PCconfig @10:29:19.25 PC.hold @10:29:19.93 PC.configure PCconfig @10:29:20.01 PS.configure PSconfig @10:29:20.12 Forearm.reconfigure LooseForearm @10:29:20.15 Forearm.hold @10:29:20.20 Forearm.configure LooseForearm @10:29:20.28 Elbow.reconfigure LooseElbow @10:29:20.32 Elbow.hold @10:29:20.41 Elbow.configure LooseElbow @10:29:20.64 SC.reconfigure SCloose @10:29:20.71 SC.hold @10:29:20.81 SC.configure SCloose @10:29:20.97 SE.reconfigure SEconfig @10:29:21.04 SE.hold @10:29:21.14 SE.configure SEconfig @10:29:21.20 CH.coast @10:29:21.25 CH.configure CHconfig @10:29:21.28 PH.coast @10:29:21.33 PH.configure PHconfig @10:29:21.36 PCR.off @10:29:21.38 PCR.pidtemp= 50,0.1,0,300,25,25 @10:29:21.41 PCR.caltemp= 2.7073,0.0373,0 @10:29:21.44 All dwarf controllers running firmware version 3.75 @10:29:21.55 Cleanly exited at 10:28:45CEST23-Aug-18 @10:29:21.58 Loading /home/esp/esp2/mission/18tun.rb mission @10:29:21.64 Preparing to sleep until +0:00:15 @10:29:21.65 GoodNews.email "Starting 18tun w/pucks in tube 2\n{}\nCan@10:29:16, 16.5C, 51% humidity, 14.6psia, 12.011V, 0.638A, 0.638A avg, 7.67W",Subject: "18tun begun" @10:29:21.73 SS.coast @10:29:21.75
Asleep til +0:00:15 @10:29:21.80 Continuing at 10:29 @10:29:21.82 CC.coast @10:29:21.96 CS.coast @10:29:22.06 PC.coast @10:29:22.12 Forearm.coast @10:29:22.18 Elbow.coast @10:29:22.24 SC.coast @10:29:22.30 SE.coast @10:29:22.61 Session Begun @10:29:22.79 Gate.power :core,:OFF @10:29:22.82 Gate.power :raw,:OFF @10:29:22.83 Shutdown @10:29:23.44 -> showlog 0 @10:29:29.14 GoodNews.email "Then, in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:106\n{}\nCan@10:29:21, 16.5C, 51% humidity, 14.6psia, 12.006V, 0.638A, 0.638A avg, 7.66W",Subject: "Asleep until 10:29" @10:29:33.19 -> showlog 100 @10:29:36.61
Powering core bus @10:29:41.43 Gate.power :raw,:ON @10:29:42.09 SPE.coast @10:29:42.16 SPE.configure SPEconfig @10:29:42.25 AS.configure ASconfig @10:29:42.36 SS.configure SSconfig @10:29:42.47 CC.configure CCconfig @10:29:42.57 CS.configure CSconfig @10:29:42.70 PC.configure PCconfig @10:29:42.80 PS.configure PSconfig @10:29:42.93 Forearm.configure LooseForearm @10:29:43.03 Elbow.configure LooseElbow @10:29:43.15 SC.configure SCloose @10:29:43.25 SE.configure SEconfig @10:29:43.31 CH.coast @10:29:43.37 CH.configure CHconfig @10:29:43.42 PH.coast @10:29:43.49 PH.configure PHconfig @10:29:43.54 PCR.off @10:29:43.58 PCR.pidtemp= 50,0.1,0,300,25,25 @10:29:43.61 PCR.caltemp= 2.7073,0.0373,0 @10:29:43.65 All dwarf controllers running firmware version 3.75 @10:29:43.80 Valve.reset @10:29:43.97 PCR.off @10:29:44.01 PCR.pidtemp= 50,0.1,0,300,25,25 @10:29:44.05 PCR.caltemp= 2.7073,0.0373,0 @10:29:44.21 Intake.select :close @10:29:44.25 Exhaust.select :close @10:29:44.28 CTV.dial :air @10:29:44.39 PSV.dial :air @10:29:44.44 CSV.dial :waste1 @10:29:44.57
Forearm.reconfigure LooseForearm @10:29:44.63 Forearm.home.seek :retract @10:29:44.71 SSV.dial :spit,avoiding: :exhaust @10:29:44.73 PS.reconfigure PSconfig @10:29:45.20 PS.home.seek 0ml @10:29:45.36 Hand.close @10:29:45.44
Elbow.jog 1500 @10:29:45.48 CS.reconfigure CSfast @10:29:45.52 SS.reconfigure SSconfig @10:29:45.68 CS.home.seek 0ml @10:29:45.72 SS.home.seek 0ml @10:29:46.13
Elbow.reconfigure LooseElbow @10:29:46.19 Elbow.home.seek :clear @10:29:50.97 SE.reconfigure SEconfig @10:29:51.03 SE.home.seek :down @10:29:51.68 SC.reconfigure SCsloppy @10:29:51.74 SC.home.seek :initial @10:29:54.44 PS.coast @10:30:02.26
Forearm.seek :retract @10:30:02.35 Elbow.seek :hammer @10:30:03.59 Forearm.reconfigure TightForearm @10:30:03.65 Forearm.seek 5 + 350 counts @10:30:04.76 Forearm.seek :retract @10:30:05.92 PC.reconfigure PCconfig @10:30:05.95
CC.reconfigure CCconfig @10:30:06.04 PC.home.seek :open @10:30:06.07
CC.home.seek :open @10:30:09.40 Phase [coreNEG] @10:30:09.42 GoodNews.email "At 10:30:09 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:106\n{}\nCan@10:30:09, 16.4C, 48% humidity, 14.6psia, 12.051V, 0.474A, 0.481A avg, 5.72W",Subject: "Started 18tun phase coreNEG" @10:30:09.45CEST23-Aug-18
==================> STARTED coreNEG (phase #1) <=== @10:30:09.47 Starting initialPurge @10:30:09.50 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:30:09.59 Hand.open @10:30:10.51 Elbow.reconfigure TightElbow @10:30:10.57 Elbow.seek FlushPuck::Garage @10:30:12.56 Elbow.reconfigure LooseElbow @10:30:12.66 Forearm.reconfigure LooseForearm @10:30:12.72 Forearm.seek :garage @10:30:13.74 Hand.close @10:30:14.66 Forearm.seek :retract @10:30:15.71 Elbow.reconfigure TightElbow @10:30:15.77 Elbow.seek CC @10:30:18.72 Elbow.reconfigure LooseElbow @10:30:18.83 Forearm.reconfigure TightForearm @10:30:18.89 Forearm.seek CC @10:30:20.15 CC.seek :closed @10:30:24.51 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.40 @10:30:24.59 Hand.open @10:30:25.43 Forearm.reconfigure LooseForearm @10:30:25.49 Forearm.seek :retract @10:30:26.49 Flushing the Collection Valve::Manifold @10:30:26.63 CSV.dial CSR @10:30:28.20 Pulling 5ml of CSR.flush @10:30:28.21 CSR.select :flush @10:30:28.36 CS.reconfigure CSconfig @10:30:28.42 CS.seek 5ml @10:30:42.24 delay 5 seconds @10:30:47.26 CSR.select :air @10:30:47.44 CS.reconfigure CSfast @10:30:47.49 CS.seek 7.5ml @10:30:54.89 CSV.dial :waste @10:30:56.40 CS.reconfigure CSconfig @10:30:56.46 CS.seek 0ml @10:31:16.68 Purging external Intake @10:31:16.80 CSV.dial :flush @10:31:17.32 CS.reconfigure CSfast @10:31:17.38 CS.seek 10ml @10:31:43.90 delay 5 seconds @10:31:48.92 CSV.dial CBV @10:31:48.96
CBV.dial CSV @10:31:49.84 CTV.dial :intake @10:31:50.70 Intake.select :open @10:31:50.83 CS.seek 0ml @10:32:18.11 Intake.select :close @10:32:18.23 CTV.dial :air @10:32:19.10 Flushing upper manifold @10:32:19.22 CSV.dial :flush @10:32:20.07 CS.seek 5ml @10:32:33.87 delay 5 seconds @10:32:38.89 CSV.dial :air @10:32:39.47 CS.seek 7ml @10:32:45.59 CSV.dial CBV @10:32:45.62
CBV.dial CSV @10:32:46.82 CTV.dial :air @10:32:46.84 CS.seek 0ml @10:33:05.77 delay 7 seconds @10:33:12.80 CS.seek 3ml @10:33:21.48 CSV.dial :waste @10:33:22.02 CS.seek 0ml @10:33:30.66 CSV.dial :flush @10:33:31.55 CS.seek 3ml @10:33:40.18 delay 5 seconds @10:33:45.20 CSV.dial :air @10:33:45.77 CS.seek 5ml @10:33:52.08 CSV.dial :air @10:33:52.11 CS.seek 0ml @10:34:06.12 delay 7 seconds @10:34:13.15 CS.seek 3ml @10:34:21.73 CSV.dial :waste @10:34:22.59 CS.seek 0ml @10:34:31.24 Garaging FlushPuck in Collection Clamp @10:34:31.32 Elbow.reconfigure TightElbow @10:34:31.38 Elbow.seek CC @10:34:31.64 Elbow.reconfigure LooseElbow @10:34:31.75 Forearm.seek CC @10:34:32.74 Hand.close @10:34:33.51 CC.seek :open @10:34:37.89 Forearm.seek :retract @10:34:38.97 Elbow.reconfigure TightElbow @10:34:39.03 Elbow.seek :garage @10:34:42.16 Elbow.reconfigure LooseElbow @10:34:42.26 Forearm.reconfigure TightForearm @10:34:42.32 Forearm.seek :garage @10:34:43.50 Hand.open @10:34:44.31 Forearm.reconfigure LooseForearm @10:34:44.37 Forearm.seek :retract @10:34:45.46 Starting mfb @10:34:45.47 pcrBAC @10:34:45.52 BACfans.email "Started mfb in tube 2 at 10:34:45CEST23-Aug-18\n{}\nCan@10:30:09, 16.4C, 48% humidity, 14.6psia, 12.051V, 0.474A, 0.481A avg, 5.72W",Subject: "pcrBAC" @10:34:45.61
Loading sh1 puck from tube 2 into Collection Clamp @10:34:45.71 Readying the MFB @10:34:45.77 Homing MFB Syringe @10:34:45.79 AFV.select :holdingCoil @10:34:46.01 ATV.dial :air2 @10:34:46.12
SC.reconfigure SCloose @10:34:46.33 Elbow.reconfigure TightElbow @10:34:46.40 Elbow.seek 2 @10:34:46.62 AS.reconfigure ASconfig @10:34:46.68 AS.home.seek :home @10:34:48.50
Forearm.reconfigure TightForearm @10:34:48.58 Forearm.seek 2 + 700 counts @10:34:49.67 SE.reconfigure SEpick @10:34:49.82 SE.seek beamBreak @10:34:52.18 AFV.select :carrierSolution @10:34:52.30 delay 2 seconds @10:34:53.04
SE.configure SEconfig @10:34:53.21 Elbow.seek 2 @10:34:53.40 Forearm.seek 2 + 300 counts @10:34:53.71 Forearm.reconfigure LooseForearm @10:34:53.77 Hand.close @10:34:54.33 AS.seek 1ml @10:34:54.52 SE.seek :down @10:34:54.74
Forearm.seek :retract @10:34:55.73 Elbow.seek CC @10:34:57.31 SE.coast @10:34:57.86
Elbow.reconfigure LooseElbow @10:34:57.98 Forearm.reconfigure TightForearm @10:34:58.04 Forearm.seek CC @10:34:59.30 CC.seek :closed @10:35:03.54 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.53 @10:35:03.55 Hand.open @10:35:04.48 Forearm.reconfigure LooseForearm @10:35:04.54 Forearm.seek :retract @10:35:05.53 SC.coast @10:35:05.58 skipping prime and sample collection @10:35:05.59 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:35:05.61 Intake.select :close @10:35:05.71 CSV.dial :waste @10:35:05.79 CS.seek 0ml @10:35:05.92 CSV.dial :air @10:35:05.94
Calibrating pressure and temperature sensors @10:35:06.02 CTV.dial :air @10:35:06.05 SSV.dial :spit,avoiding: :exhaust @10:35:06.09 SS.reconfigure SSfast @10:35:06.16 SS.seek 5ml @10:35:06.85 CS.seek 10ml @10:35:09.34 SSV.dial CBV @10:35:09.37
CBV.dial SSV @10:35:10.90 delay 3.8 seconds @10:35:14.83 SSV.dial :spit @10:35:16.34 SS.seek 0ml @10:35:19.06 SSV.dial CBV @10:35:20.57 SSV.dial CBV,avoiding: :exhaust @10:35:20.60
CBV.dial SSV @10:35:20.62 CTV.dial :air @10:35:20.64 delay 15 seconds @10:35:35.68 SSV.dial :spit,avoiding: :exhaust @10:35:37.20 CTV.dial CSV,avoiding: :intake @10:35:37.25
SS.reconfigure SSexhale @10:35:37.35 SS.seek 0ml @10:35:37.42 SSV.dial CBV,avoiding: :exhaust @10:35:39.01 CBV.dial between SSV and waste2 @10:35:39.44 CTV.dial CSV,avoiding: :air @10:35:39.47
CSV.dial CTV @10:35:40.10 CS.reconfigure CSpressurize @10:35:40.17 CS.seek 0ml @10:35:47.26 delay 1 second @10:35:48.28 AFV.select :holdingCoil @10:35:48.38 delay 1 second @10:35:49.40 ATV.dial :waste @10:35:50.57 AS.reconfigure ASnorm @10:35:50.63 AS.home.seek :empty @10:36:06.39
CSV.dial :air @10:36:06.92 CS.reconfigure CSfast @10:36:06.99 CS.seek 10ml @10:36:33.30 CSV.dial between air and CTV @10:36:33.80 CS.seek 3.413ml @10:36:41.86 ATV.dial :waste @10:36:41.88 ARV.dial between carrier and air1 @10:36:41.99 ASV.dial :waste1 @10:36:51.53 CTV.dial CSV,avoiding: :air @10:36:51.55
CSV.dial CTV @10:36:51.92 CS.reconfigure CSpressurize @10:36:51.99 CS.seek 0ml @10:37:01.45 CSV.dial :air @10:37:02.10 CS.reconfigure CSfast @10:37:02.17 CS.seek 10ml @10:37:28.67 CSV.dial between air and CTV @10:37:29.46 CS.seek 2.154ml @10:37:50.43 CTV.dial CSV,avoiding: :air @10:37:50.46
CSV.dial CTV @10:37:50.92 CS.reconfigure CSpressurize @10:37:50.99 CS.seek 0ml @10:37:52.93 BubblePoint: Intake @69.7psia, Outlet @14.8psia @10:37:52.94 CBV.dial SSV @10:37:53.32 SS.reconfigure SSconfig @10:37:53.38 SS.seek 10ml @10:37:59.46 delay 15 seconds @10:38:14.58 Evacuated: Intake @28.6psia, Outlet @29.1psia @10:38:14.59 SSV.dial :spit,avoiding: :exhaust @10:38:16.12 SS.reconfigure SSexhale @10:38:16.19 SS.seek 0ml @10:38:21.35 SS.reconfigure SSfast @10:38:21.42 SS.seek 5ml @10:38:24.24 SSV.dial CBV,avoiding: :exhaust @10:38:24.27
CBV.dial SSV @10:38:25.80 CTV.dial :air,avoiding: :intake @10:38:27.37 CSV.dial CTV @10:38:27.41
SS.seek 10ml @10:38:28.41 CSV.dial :air @10:38:28.90 CS.reconfigure CSfast @10:38:28.96 CS.seek 0ml @10:38:30.46
SSV.dial :spit,avoiding: :exhaust @10:38:31.98 SS.reconfigure SSexhale @10:38:32.05 SS.seek 0ml @10:38:34.08 CS.coast @10:38:37.21
SS.reconfigure SSfast @10:38:37.29 SS.seek 5ml @10:38:40.15 SSV.dial CBV,avoiding: :exhaust @10:38:40.18
CBV.dial SSV @10:38:42.72 SSV.dial :spit,avoiding: :exhaust @10:38:44.26 SS.reconfigure SSconfig @10:38:44.33 SS.seek 0ml @10:38:47.36 SS.coast @10:38:47.42 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:38:47.43 Intake.select :close @10:38:47.53 CSV.dial :waste @10:38:47.65 SSV.dial CBV,avoiding: :exhaust @10:38:47.68
CBV.dial SSV @10:38:48.43 CS.seek 0ml @10:38:48.52 CSV.dial :air @10:38:49.19
CTV.dial :air @10:38:49.20 delay 15 seconds @10:38:49.37 CS.seek 10ml @10:39:04.23
SSV.dial :spit,avoiding: :exhaust @10:39:05.76 CTV.dial CSV,avoiding: :intake @10:39:05.80
SS.reconfigure SSexhale @10:39:05.90 SS.seek 0ml @10:39:06.40 SSV.dial CBV,avoiding: :exhaust @10:39:15.89 CBV.dial between SSV and waste2 @10:39:16.31 CTV.dial CSV,avoiding: :air @10:39:16.33
CSV.dial CTV @10:39:16.86 CS.reconfigure CSpressurize @10:39:16.93 CS.seek 0ml @10:39:43.05 CSV.dial :air @10:39:43.59 CS.reconfigure CSfast @10:39:43.65 CS.seek 10ml @10:40:10.02 CSV.dial between air and CTV @10:40:10.40 CS.seek 3.383ml @10:40:28.19 CTV.dial CSV,avoiding: :air @10:40:28.22
CSV.dial CTV @10:40:28.58 CS.reconfigure CSpressurize @10:40:28.65 CS.seek 0ml @10:40:38.02 CSV.dial :air @10:40:38.56 CS.reconfigure CSfast @10:40:38.62 CS.seek 10ml @10:41:04.92 CSV.dial between air and CTV @10:41:05.70 CS.seek 2.142ml @10:41:26.72 CTV.dial CSV,avoiding: :air @10:41:26.75
CSV.dial CTV @10:41:27.09 CS.reconfigure CSpressurize @10:41:27.16 CS.seek 0ml @10:41:29.21 BubblePoint: Intake @70.5psia, Outlet @16.0psia @10:41:29.22 CBV.dial SSV @10:41:29.59 SS.reconfigure SSconfig @10:41:29.65 SS.seek 10ml @10:41:35.74 delay 15 seconds @10:41:50.86 Evacuated: Intake @28.9psia, Outlet @29.7psia @10:41:50.87 SSV.dial :spit,avoiding: :exhaust @10:41:52.40 SS.reconfigure SSexhale @10:41:52.47 SS.seek 0ml @10:41:57.84 SS.reconfigure SSfast @10:41:57.91 SS.seek 5ml @10:42:00.57 SSV.dial CBV,avoiding: :exhaust @10:42:00.60
CBV.dial SSV @10:42:02.13 CTV.dial :air,avoiding: :intake @10:42:03.67 CSV.dial CTV @10:42:03.71
SS.seek 10ml @10:42:04.71 CSV.dial :air @10:42:05.23 CS.reconfigure CSfast @10:42:05.29 CS.seek 0ml @10:42:06.54
SSV.dial :spit,avoiding: :exhaust @10:42:08.05 SS.reconfigure SSexhale @10:42:08.12 SS.seek 0ml @10:42:10.27 CS.coast @10:42:13.31
SS.reconfigure SSfast @10:42:13.38 SS.seek 5ml @10:42:16.38 SSV.dial CBV,avoiding: :exhaust @10:42:16.41
CBV.dial SSV @10:42:18.94 SSV.dial :spit,avoiding: :exhaust @10:42:20.44 SS.reconfigure SSconfig @10:42:20.50 SS.seek 0ml @10:42:23.79 SS.coast @10:42:23.85 Lysing: Adding 1.5ml lysis @10:42:23.86 CBV.dial between CSV and waste1,avoiding: :DBV @10:42:25.58 CTV.dial CSV @10:42:27.11 CSV.dial CSR @10:42:28.02 Pulling 1ml of CSR.lysis @10:42:28.03 CSR.select :lysis @10:42:28.17 CS.reconfigure CSconfig @10:42:28.23 CS.seek 1ml @10:42:31.84 delay 5 seconds @10:42:36.86 CSR.select :air @10:42:37.04 CS.seek 6ml @10:42:50.78 delay 2 seconds @10:42:52.80 CSV.dial :waste1 @10:42:54.28 CS.reconfigure CSfast @10:42:54.34 CS.seek :empty @10:43:10.33 CSV.dial CSR @10:43:11.88 Pulling 1.5ml of CSR.lysis @10:43:11.89 CSR.select :lysis @10:43:12.03 CS.reconfigure CSconfig @10:43:12.09 CS.seek 1.5ml @10:43:16.94 delay 5 seconds @10:43:21.96 CSR.select :air @10:43:22.13 CS.seek 4.5ml @10:43:30.91 delay 2 seconds @10:43:32.93 CSV.dial CTV @10:43:33.44 CS.seek 1.275ml @10:43:42.91 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @10:43:42.94 CH.seek 85C,8:00 @10:47:37.45 CH.hold 5:00,85C @10:52:37.62 Collection Puck Heater at 84.6C after lysing @10:52:37.64 CS.seek 0ml @10:52:41.88 CTV.dial between spare and air,avoiding: :intake @10:52:43.09 CSV.dial :air @10:52:43.60 CS.seek 5ml @10:52:57.34 CSV.dial CTV @10:52:57.87 delay 2 seconds @10:52:59.89 CSV.dial :air @10:53:00.41 delay 2 seconds @10:53:02.43 CSV.dial CTV @10:53:02.96 delay 2 seconds @10:53:04.98 CSV.dial :air @10:53:05.51 delay 2 seconds @10:53:07.54 CS.seek 0ml @10:53:21.32 Recovering Lysate into Collection syringe @10:53:21.33 CSV.dial CBV @10:53:22.52 CBV.dial CSV @10:53:22.94 CS.seek 5ml @10:53:36.91 delay 5 seconds @10:53:41.93 CTV.dial :air @10:53:42.54 CS.seek 7ml @10:53:49.04 delay 5 seconds @10:53:54.06 CSV.dial :air2 @10:53:54.57 CS.seek 10ml @10:54:03.29 CSV.dial between air and CTV,avoiding: CBV @10:54:05.06 Lysing Complete @10:54:05.15 Storing sh1 puck in Collection Clamp to tube 1 @10:54:05.33 SC.reconfigure SCtight @10:54:05.39 SC.seek 1 @10:54:07.52 SC.reconfigure SCloose @10:54:07.73 Elbow.reconfigure TightElbow @10:54:07.80 Elbow.seek 1 @10:54:09.69 Forearm.reconfigure TightForearm @10:54:09.75 Forearm.seek 1 + 700 counts @10:54:10.81 SE.reconfigure SEpick @10:54:10.90 SE.seek beamBreak @10:54:13.91 SE.configure SEconfig @10:54:13.98 SE.seek :place @10:54:14.49 Forearm.reconfigure LooseForearm @10:54:14.55 Forearm.seek :retract @10:54:15.50 Elbow.seek CC @10:54:17.22 Elbow.reconfigure LooseElbow @10:54:17.33 Forearm.seek CC @10:54:18.34 Hand.close @10:54:19.16 CC.seek :open @10:54:23.63 Forearm.seek :retract @10:54:24.66 Elbow.reconfigure TightElbow @10:54:24.72 Elbow.seek 1 @10:54:26.63 Elbow.reconfigure LimpElbow @10:54:26.70 Forearm.seek 1 @10:54:27.74 Elbow.hold @10:54:27.81 Elbow.reconfigure TightElbow @10:54:27.87 Hand.open @10:54:28.78 Forearm.force -20 @10:54:28.83 Forearm.seek 1 + 349 counts @10:54:29.15 Forearm.reconfigure TightForearm @10:54:29.21 Forearm.seek 1 + 700 counts @10:54:29.59 SE.seek :down @10:54:30.72
Forearm.reconfigure LooseForearm @10:54:30.78 Forearm.seek :retract @10:54:32.39 SE.coast @10:54:32.44
SC.coast @10:54:32.49 Tranferring lysate to PRV @10:54:32.50 CSV.dial :air @10:54:32.84 CS.seek 10ml @10:54:32.94 CSV.dial between air and CTV @10:54:33.31 AIV.dial :bypass @10:54:33.40 Beginning samplePassC @10:54:33.41 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:54:33.50 Hand.open @10:54:33.90 Elbow.seek FlushPuck::Garage @10:54:35.94 Elbow.reconfigure LooseElbow @10:54:36.05 Forearm.seek :garage @10:54:37.08 Hand.close @10:54:38.34 Forearm.seek :retract @10:54:39.36 Elbow.reconfigure TightElbow @10:54:39.42 Elbow.seek CC @10:54:42.36 Elbow.reconfigure LooseElbow @10:54:42.47 Forearm.reconfigure TightForearm @10:54:42.53 Forearm.seek CC @10:54:43.76 CC.seek :closed @10:54:48.04 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.43 @10:54:48.14 Hand.open @10:54:48.97 Forearm.reconfigure LooseForearm @10:54:49.03 Forearm.seek :retract @10:54:50.02 AWV.select :air @10:54:50.04 AIV.dial :bypass @10:54:50.07 PSV.dial PSR @10:54:51.62 Pulling 1.5ml of PSR.flush @10:54:51.63 PSR.select :flush @10:54:51.78 PS.seek 1.5ml @10:55:08.31 delay 5 seconds @10:55:13.33 PSV.dial :waste1 @10:55:13.87 PS.seek 0ml @10:55:30.26 PSV.dial PSR @10:55:30.92 PSR.select :air @10:55:31.07 PS.seek 2.5ml @10:55:58.02 PRV.dial CBV @10:55:58.05
CBV.dial PRV @10:55:58.97 PSV.dial :mixloop @10:56:00.15 CTV.dial :air,avoiding: :intake @10:56:00.16 PTV.dial :PRVmixing @10:56:00.26 PS.seek 0ml @10:56:27.23 PSV.dial :air @10:56:27.76 PS.seek 2.5ml @10:56:54.45 PSV.dial :mixloop @10:56:54.98 PS.seek 0ml @10:57:21.70 PSV.dial :air @10:57:22.20 PS.seek 2.5ml @10:57:48.98 PSV.dial :mixloop @10:57:49.50 PS.seek 0ml @10:58:16.37 Garaging FlushPuck in Collection Clamp @10:58:16.45 Elbow.reconfigure TightElbow @10:58:16.51 Elbow.seek CC @10:58:16.77 Elbow.reconfigure LooseElbow @10:58:16.88 Forearm.seek CC @10:58:17.87 Hand.close @10:58:18.66 CC.seek :open @10:58:23.06 Forearm.seek :retract @10:58:24.08 Elbow.reconfigure TightElbow @10:58:24.14 Elbow.seek :garage @10:58:27.34 Elbow.reconfigure LooseElbow @10:58:27.45 Forearm.reconfigure TightForearm @10:58:27.51 Forearm.seek :garage @10:58:28.68 Hand.open @10:58:29.47 Forearm.reconfigure LooseForearm @10:58:29.97 Forearm.seek :retract @10:58:31.01 Connecting PS to CS to transfer sample @10:58:31.02 CBV.dial AIV @10:58:31.04 AIV.dial :bypass @10:58:31.07 PSV.dial :mixloop @10:58:31.09 PTV.dial :PRVmixing @10:58:31.12 PRV.dial AIV @10:58:31.14 CTV.dial :air,avoiding: :intake @10:58:31.17 Loading lyfil puck from tube 2 into Collection Clamp @10:58:31.41 SC.reconfigure SCtight @10:58:31.48 SC.seek 2 @10:58:33.67 SC.reconfigure SCloose @10:58:33.89 Elbow.reconfigure TightElbow @10:58:33.95 Elbow.seek 2 @10:58:35.98 Forearm.reconfigure TightForearm @10:58:36.04 Forearm.seek 2 + 700 counts @10:58:37.08 SE.reconfigure SEpick @10:58:37.17 SE.seek beamBreak @10:58:40.37 SE.configure SEconfig @10:58:40.54 Elbow.seek 2 @10:58:40.72 Forearm.seek 2 + 300 counts @10:58:40.99 Forearm.reconfigure LooseForearm @10:58:41.05 Hand.close @10:58:41.80 SE.seek :down @10:58:42.01
Forearm.seek :retract @10:58:42.96 Elbow.seek CC @10:58:44.68 Elbow.reconfigure LooseElbow @10:58:44.81 SE.coast @10:58:44.83
Forearm.reconfigure TightForearm @10:58:44.92 Forearm.seek CC @10:58:46.19 CC.seek :closed @10:58:50.48 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.48 @10:58:50.49 Hand.open @10:58:51.34 Forearm.reconfigure LooseForearm @10:58:51.40 Forearm.seek :retract @10:58:52.39 SC.coast @10:58:52.44 CTV.dial CSV,avoiding: :intake @10:58:54.00 CSV.dial CTV @10:58:54.34 CS.seek 5ml @10:59:08.14 delay 1:20 @11:00:28.17 CS.seek 2.5ml @11:00:35.51 delay 40 seconds @11:01:15.54 CS.seek 0ml @11:01:22.95 delay 1:00 @11:02:22.97 PSV.dial :air @11:02:23.47 PS.seek 2.5ml @11:02:50.33 PSV.dial :mixloop @11:02:50.86 delay 3 seconds @11:02:53.88 PSV.dial :air @11:02:54.40 delay 2 seconds @11:02:56.42 PSV.dial :mixloop @11:02:56.94 delay 3 seconds @11:02:59.96 PSV.dial :waste1 @11:03:01.50 PS.seek :empty @11:03:28.36 CBV.dial :waste1 @11:03:28.91 delay 5 seconds @11:03:33.93 CBV.dial AIV @11:03:34.47 delay 10 seconds @11:03:44.50 CBV.dial :waste1 @11:03:45.49 delay 10 seconds @11:03:55.52 CBV.dial AIV @11:03:56.05 delay 10 seconds @11:04:06.08 CTV.dial CSV,avoiding: :intake @11:04:06.09 CBV.dial between AIV and waste1 @11:04:06.49 CSV.dial :air @11:04:07.02 CS.seek 10ml @11:04:33.54 CSV.dial CTV @11:04:34.06 CS.seek 0ml @11:05:00.76 CBV.dial :waste1 @11:05:01.13 delay 10 seconds @11:05:11.16 CTV.dial :air,avoiding: :intake @11:05:12.75 Storing lyfil puck in Collection Clamp to tube 1 @11:05:12.92 SC.reconfigure SCtight @11:05:12.98 SC.seek 1 @11:05:15.13 SC.reconfigure SCloose @11:05:15.33 Elbow.reconfigure TightElbow @11:05:15.40 Elbow.seek 1 @11:05:17.29 Forearm.reconfigure TightForearm @11:05:17.35 Forearm.seek 1 + 700 counts @11:05:18.37 SE.reconfigure SEpick @11:05:18.46 SE.seek beamBreak @11:05:21.64 SE.configure SEconfig @11:05:21.80 Elbow.reconfigure LimpElbow @11:05:21.86 Forearm.reconfigure LooseForearm @11:05:21.93 Forearm.seek 1 @11:05:22.23 Elbow.hold @11:05:22.32 Elbow.reconfigure TightElbow @11:05:22.42 SE.seek 0.04 Pucks below Top Plate @11:05:22.84 Forearm.force -20 @11:05:22.89 Forearm.seek 1 + 346 counts @11:05:23.23 Forearm.reconfigure TightForearm @11:05:23.29 Forearm.seek 1 + 700 counts @11:05:23.56 SE.seek 1.04 Pucks below Top Plate @11:05:24.14 Forearm.reconfigure LooseForearm @11:05:24.20 Forearm.seek :retract @11:05:25.14 Elbow.seek CC @11:05:26.88 Elbow.reconfigure LooseElbow @11:05:26.99 Forearm.seek CC @11:05:28.00 Hand.close @11:05:28.79 CC.seek :open @11:05:33.30 Forearm.seek :retract @11:05:34.31 Elbow.reconfigure TightElbow @11:05:34.37 Elbow.seek 1 @11:05:36.25 Elbow.reconfigure LimpElbow @11:05:36.32 Forearm.seek 1 @11:05:37.31 Elbow.hold @11:05:37.39 Elbow.reconfigure TightElbow @11:05:37.44 Hand.open @11:05:38.29 Forearm.force -20 @11:05:38.34 Forearm.seek 1 + 350 counts @11:05:38.65 Forearm.reconfigure TightForearm @11:05:39.16 Forearm.seek 1 + 700 counts @11:05:39.46 SE.seek :down @11:05:40.59
Forearm.reconfigure LooseForearm @11:05:40.65 Forearm.seek :retract @11:05:42.16 SE.coast @11:05:42.20
SC.coast @11:05:42.26 PSV.dial :air @11:05:43.49 PS.seek 0.499ml @11:05:49.47 delay 3 seconds @11:05:52.49 PSV.dial :mixloop @11:05:53.00 PRV.dial :air @11:05:53.51 delay 3 seconds @11:05:56.53 PRV.dial CBV @11:05:57.05 CBV.dial AIV @11:05:57.58 PRV.dial between CBV and air @11:05:57.95 PRV.dial :air @11:05:58.36 PS.reconfigure PSslow @11:05:58.42 PS.seek 0.599ml @11:06:00.99 PRV.dial :waste2 @11:06:01.84 PS.reconfigure PSconfig @11:06:01.90 PS.seek :empty @11:06:08.94 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:06:09.02 Hand.open @11:06:09.41 Elbow.seek FlushPuck::Garage @11:06:11.44 Elbow.reconfigure LooseElbow @11:06:11.55 Forearm.seek :garage @11:06:12.59 Hand.close @11:06:13.40 Forearm.seek :retract @11:06:14.44 Elbow.reconfigure TightElbow @11:06:14.50 Elbow.seek CC @11:06:17.44 Elbow.reconfigure LooseElbow @11:06:17.55 Forearm.reconfigure TightForearm @11:06:17.61 Forearm.seek CC @11:06:18.85 CC.seek :closed @11:06:23.10 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.49 @11:06:23.17 Hand.open @11:06:23.98 Forearm.reconfigure LooseForearm @11:06:24.04 Forearm.seek :retract @11:06:25.14 Clearing PML for modified lysate @11:06:25.16 CTV.dial :air,avoiding: :intake @11:06:25.18 PRV.dial :waste2 @11:06:25.20 PTV.dial :PRV,avoiding: :puck @11:06:26.05 delay 2 seconds @11:06:28.07 PTV.dial :PRVmixing,avoiding: :puck @11:06:28.92 delay 2 seconds @11:06:30.94 PSV.dial :PML @11:06:30.96 delay 2 seconds @11:06:33.00 PRV.dial :air @11:06:33.84 delay 2 seconds @11:06:35.91 PS.seek 1.499ml @11:06:52.31 delay 5 seconds @11:06:57.34 PS.seek 2.5ml @11:07:08.64 PSV.dial :air @11:07:09.61 PS.seek 0ml @11:07:36.53 mixing lysate with speDil @11:07:36.54 PSV.dial :PML @11:07:37.08 CBV.dial :PRV @11:07:37.09 PRV.dial :air @11:07:37.12 delay 2 seconds @11:07:39.15 PRV.dial :speDil @11:07:39.67 delay 2 seconds @11:07:41.75 PS.reconfigure PSslow1 @11:07:41.81 PS.seek 0.225ml @11:08:05.63 delay 5 seconds @11:08:10.65 PRV.dial CBV @11:08:11.52 delay 2 seconds @11:08:13.59 PS.seek 0.475ml @11:08:39.28 delay 5 seconds @11:08:44.30 PRV.dial :air @11:08:44.81 delay 2 seconds @11:08:46.84 PS.seek 0.965ml @11:09:37.06 delay 10 seconds @11:09:47.08 PRV.dial CBV @11:09:47.61 CTV.dial :air,avoiding: :intake @11:09:47.62 PSV.dial :air @11:09:48.14 PS.reconfigure PSconfig @11:09:48.20 PS.seek 2.5ml @11:10:05.03 delay 3 seconds @11:10:08.05 PSV.dial PTV @11:10:08.89 PTV.dial :PRV,avoiding: :puck @11:10:09.74 PS.seek 0ml @11:10:36.65 PSV.dial :air @11:10:37.50 PS.seek 2.5ml @11:11:04.31 delay 3 seconds @11:11:07.33 PSV.dial PTV @11:11:08.17 PTV.dial :PRV,avoiding: :puck @11:11:08.19 PS.seek 0ml @11:11:35.13 Pushing modified lysate into AIV Injection Loop @11:11:35.14 CBV.dial AIV @11:11:35.16 AIV.dial :bypass @11:11:35.19 PRV.dial CBV @11:11:35.21 PSV.dial :air @11:11:36.06 PS.seek 2.5ml @11:12:02.78 delay 3 seconds @11:12:05.80 PSV.dial :PML @11:12:06.31 PTV.dial :PRVmixing,avoiding: :puck @11:12:07.22 PS.reconfigure PSslow1 @11:12:07.28 PS.seek 1.74ml @11:13:24.66 delay 5 seconds @11:13:29.68 AIV.dial :sample @11:13:30.32 AWV.select :waste @11:13:30.49 PS.reconfigure PSslow2 @11:13:30.55 PS.seek 1.22ml @11:15:17.82 delay 15 seconds @11:15:32.84 AIV.dial :bypass @11:15:33.48 PS.reconfigure PSconfig @11:15:33.54 PS.seek 0ml @11:15:47.03 PSV.dial :air @11:15:47.55 PS.seek 2.5ml @11:16:01.88 Session Begun @11:16:13.73 -> showlog 100 @11:16:14.40
PSV.dial :PML @11:16:14.95 PS.seek 0ml @11:16:41.81 AIV.dial :coilToMFB @11:16:41.83 AWV.select :air @11:16:42.03 Extracting Nucleic Acid from AIV @11:16:42.07 SPE.hold 2:46:40,55C @11:16:42.12
Beginning samplePassD @11:16:42.18 ASV.dial :SPEdelivery @11:16:42.35
AWV.select :air @11:16:42.37 AIV.dial :bypass @11:16:42.40 PSV.dial PSR @11:16:42.69 delay 20 seconds @11:16:43.73
Pulling 1.5ml of PSR.killMFB @11:16:43.74 PSR.select :killMFB @11:16:43.90 PS.seek 1.5ml @11:17:01.00 delay 5 seconds @11:17:02.71 primeLysateINT @11:17:02.72 AWV.select :air @11:17:02.74 AIV.dial :coilToMFB @11:17:02.77 ATV.dial AIV @11:17:04.68 AS.reconfigure ASslow1 @11:17:05.20 AS.seek 0.299ml @11:17:06.02
PSV.dial :waste1 @11:17:06.58 PS.seek 0ml @11:17:23.75 PSV.dial PSR @11:17:24.32 PSR.select :air @11:17:24.46 PS.seek 2.5ml @11:17:52.47 PRV.dial CBV @11:17:52.51
CBV.dial PRV @11:17:52.53 PSV.dial :mixloop @11:17:53.79 CTV.dial :air,avoiding: :intake @11:17:53.80 PTV.dial :PRVmixing @11:17:53.86 PS.seek 0ml @11:18:05.87 delay 10 seconds @11:18:15.90 ATV.dial :air2 @11:18:16.81 AS.seek 0.329ml @11:18:21.81
PSV.dial PSR @11:18:23.12 Pulling 1.5ml of PSR.flush @11:18:23.13 ATV.dial :waste2 @11:18:23.18
PSR.select :flush @11:18:23.36 PS.seek 1.5ml @11:18:23.80 AS.reconfigure ASnorm @11:18:23.88 AS.seek :empty @11:18:40.48
delay 5 seconds @11:18:40.94 Lysate primed! @11:18:40.95 AFV.select :holdingCoil @11:18:40.97 ATV.dial :air2 @11:18:41.64 AS.reconfigure ASslow1 @11:18:41.72 AS.seek 0.069ml @11:18:45.50
PSV.dial :waste1 @11:18:46.05 PS.seek 0ml @11:18:56.16 delay 3 seconds @11:18:59.18 ATV.dial :AIV @11:19:00.09 AS.seek 0.469ml @11:19:03.28
PSV.dial PSR @11:19:03.85 PSR.select :air @11:19:04.00 PS.seek 2.5ml @11:19:32.07 PRV.dial CBV @11:19:32.10
CBV.dial PRV @11:19:32.13 PSV.dial :mixloop @11:19:33.38 CTV.dial :air,avoiding: :intake @11:19:33.39 PTV.dial :PRVmixing @11:19:33.44 PS.seek 0ml @11:20:01.50 PSV.dial PSR @11:20:02.79 Pulling 1.5ml of PSR.flush @11:20:02.80 PSR.select :flush @11:20:02.96 PS.seek 1.5ml @11:20:19.94 delay 5 seconds @11:20:20.72 delay 5 seconds @11:20:24.96
PSV.dial :waste1 @11:20:25.51 PS.seek 0ml @11:20:25.78 Solid Phase Extraction Heater at 46.9C @11:20:25.79 ATV.dial ASV @11:20:26.23 ASV.dial :SPEdelivery @11:20:26.33 AS.seek 0.041ml @11:20:42.53
PSV.dial PSR @11:20:43.08 PSR.select :air @11:20:43.23 PS.seek 2.5ml @11:21:10.88 PRV.dial CBV @11:21:10.91
CBV.dial PRV @11:21:10.94 PSV.dial :mixloop @11:21:12.18 CTV.dial :air,avoiding: :intake @11:21:12.19 PTV.dial :PRVmixing @11:21:12.25 PS.seek 0ml @11:21:39.72 PSV.dial PSR @11:21:41.00 Pulling 1.5ml of PSR.flush @11:21:41.01 PSR.select :flush @11:21:41.18 PS.seek 1.5ml @11:21:52.70 delay 10 seconds @11:21:58.01
delay 5 seconds @11:22:02.72 rinseLysateLineINT @11:22:02.73 AFV.select :carrierSolution @11:22:02.87 delay 2 seconds @11:22:03.03
PSV.dial :waste1 @11:22:03.55 PS.seek 0ml @11:22:04.89 AS.reconfigure ASconfig @11:22:04.97 AS.seek 1ml @11:22:20.22
PSV.dial PSR @11:22:21.23 PSR.select :air @11:22:21.38 PS.seek 2.5ml @11:22:48.54 PRV.dial CBV @11:22:48.58
CBV.dial PRV @11:22:48.60 PSV.dial :mixloop @11:22:49.82 CTV.dial :air,avoiding: :intake @11:22:49.83 PTV.dial :PRVmixing @11:22:49.89 PS.reconfigure PSslow @11:22:49.96 PS.seek 1.2ml @11:23:00.42 delay 1 second @11:23:01.44 AFV.select :holdingCoil @11:23:01.54 delay 1 second @11:23:02.56 ATV.dial AIV @11:23:03.23 AIV.dial :coilToMFB @11:23:03.24 AWV.select :waste @11:23:03.39 AS.reconfigure ASslow0 @11:23:03.45 AS.seek 0ml @11:23:16.82
PSV.dial :waste1 @11:23:18.35 PS.reconfigure PSconfig @11:23:18.43 PS.seek 0ml @11:23:31.75 PSV.dial :air @11:23:32.97 PS.seek 2.5ml @11:23:59.62 PSV.dial :mixloop @11:24:00.15 PS.reconfigure PSslow @11:24:00.23 PS.seek 0ml @11:24:44.37 ATV.dial :air2 @11:24:45.09 AS.reconfigure ASslow1 @11:24:45.16 AS.seek 0.05ml @11:24:51.41
PSV.dial :air @11:24:51.95 PS.reconfigure PSconfig @11:24:52.03 PS.seek 2.5ml @11:24:55.51 ATV.dial :AIV @11:24:56.39 AS.seek 0.01ml @11:25:04.75 ATV.dial :waste @11:25:06.44 AS.seek :empty @11:25:08.88 ethanol wash @11:25:08.89 ATV.dial :air2 @11:25:10.21 AS.reconfigure ASslow2 @11:25:10.29 AS.seek 0.009ml @11:25:18.81
PSV.dial :mixloop @11:25:19.35 PS.reconfigure PSslow @11:25:19.42 PS.seek 0ml @11:25:20.97 delay 3 seconds @11:25:23.99 Pulling 0.4ml of Ethanol Wash @11:25:24.00 ATV.dial :ethanol @11:25:24.85 AS.reconfigure ASslow1 @11:25:24.93 AS.seek 0.409ml @11:26:10.76
Cleaning Collection Syringe @11:26:10.77 CSV.dial CSR @11:26:11.35 Pulling 4ml of CSR.flush @11:26:11.36 CSR.select :flush @11:26:11.52 CS.seek 4ml @11:26:22.82 delay 5 seconds @11:26:27.84 CSR.select :air @11:26:27.99 CS.seek 10ml @11:26:44.32 CSV.dial CTV @11:26:44.35
CTV.dial CSV @11:26:45.65 delay 3 seconds @11:26:45.97
CBV.dial :waste1 @11:26:46.52 CS.seek 0ml @11:26:48.68 ATV.dial :air1 @11:26:49.08 delay 3 seconds @11:26:52.15 AS.seek 0.434ml @11:26:57.45 delay 3 seconds @11:27:00.48 ATV.dial :ethanol @11:27:01.11 AS.seek 0.419ml @11:27:04.42 delay 3 seconds @11:27:07.44 ATV.dial ASV @11:27:08.80 AS.seek 0.092ml @11:27:13.03
CSV.dial CSR @11:27:13.63 Pulling 4ml of CSR.flush @11:27:13.64 CSR.select :flush @11:27:13.80 CS.seek 4ml @11:27:25.10 delay 5 seconds @11:27:30.12 CSR.select :air @11:27:30.26 CS.seek 10ml @11:27:46.54 CSV.dial CTV @11:27:46.57
CTV.dial CSV @11:27:47.51 CBV.dial :waste1 @11:27:47.60 CS.seek 0ml @11:28:14.27 Garaging FlushPuck in Collection Clamp @11:28:14.38 Elbow.reconfigure TightElbow @11:28:14.44 Elbow.seek CC @11:28:14.73 Elbow.reconfigure LooseElbow @11:28:14.84 delay 3 seconds @11:28:14.92
Forearm.seek CC @11:28:15.93 Hand.close @11:28:16.69 CC.seek :open @11:28:17.87 ATV.dial :waste2 @11:28:18.66 AS.reconfigure ASconfig @11:28:18.73 AS.seek :empty @11:28:21.08
Forearm.seek :retract @11:28:22.11 Elbow.reconfigure TightElbow @11:28:22.18 Elbow.seek :garage @11:28:24.40 ATV.dial :air2 @11:28:24.83 AS.reconfigure ASslow1 @11:28:24.90 AS.seek 1ml @11:28:25.20
Elbow.reconfigure LooseElbow @11:28:25.32 Forearm.reconfigure TightForearm @11:28:25.39 Forearm.seek :garage @11:28:26.59 Hand.open @11:28:27.38 Forearm.reconfigure LooseForearm @11:28:27.45 Forearm.seek :retract @11:28:28.52 Skipping Whole Cell Archive @11:28:28.53 Starting finalPurge @11:28:28.56 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:28:28.67 Hand.open @11:28:29.00 Elbow.reconfigure TightElbow @11:28:29.07 Elbow.seek FlushPuck::Garage @11:28:29.21 Elbow.reconfigure LooseElbow @11:28:29.34 Forearm.seek :garage @11:28:30.38 Hand.close @11:28:31.30 Forearm.seek :retract @11:28:32.36 Elbow.reconfigure TightElbow @11:28:32.43 Elbow.seek CC @11:28:35.35 Elbow.reconfigure LooseElbow @11:28:35.48 Forearm.reconfigure TightForearm @11:28:35.54 Forearm.seek CC @11:28:36.78 CC.seek :closed @11:28:41.08 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.38 @11:28:41.18 Hand.open @11:28:42.00 Forearm.reconfigure LooseForearm @11:28:42.06 Forearm.seek :retract @11:28:43.07 Flushing the Collection Valve::Manifold @11:28:43.21 CSV.dial CSR @11:28:43.80 Pulling 5ml of CSR.flush @11:28:43.81 CSR.select :flush @11:28:43.97 CS.seek 5ml @11:28:57.72 delay 5 seconds @11:29:02.74 CSR.select :air @11:29:03.41 CS.reconfigure CSfast @11:29:03.48 CS.seek 7.5ml @11:29:10.84 CSV.dial :waste @11:29:12.38 CS.reconfigure CSconfig @11:29:12.46 CS.seek 0ml @11:29:32.60 Flushing lower manifold's waste1 port @11:29:32.75 CSV.dial :flush @11:29:33.27 CS.reconfigure CSfast @11:29:33.35 CS.seek 5ml @11:29:47.07 delay 5 seconds @11:29:52.09 CSV.dial :air @11:29:52.80 CS.seek 7ml @11:29:58.88 CSV.dial CTV @11:29:58.91
CTV.dial CSV @11:29:59.48 CBV.dial :waste1 @11:29:59.50 CS.seek 0ml @11:30:18.44 Flushing lower manifold's waste2b port @11:30:18.58 CSV.dial :flush @11:30:19.48 CS.seek 5ml @11:30:33.29 delay 5 seconds @11:30:38.31 CSV.dial :air @11:30:38.93 CS.seek 7ml @11:30:45.16 CSV.dial CTV @11:30:45.18
CTV.dial CSV @11:30:45.73 CBV.dial :waste2b @11:30:46.60 CS.seek 0ml @11:31:05.32 Flushing lower manifold's waste1 port @11:31:05.33 CSV.dial CSR @11:31:06.05 Pulling 4ml of CSR.mfbkill @11:31:06.06 CSR.select :mfbkill @11:31:06.22 CS.reconfigure CSconfig @11:31:06.29 CS.seek 4ml @11:31:17.48 delay 5 seconds @11:31:22.50 CSR.select :air @11:31:22.64 CS.reconfigure CSfast @11:31:22.71 CS.seek 10ml @11:31:39.01 CSV.dial CTV @11:31:39.04
CTV.dial CSV @11:31:39.59 CBV.dial :waste1 @11:31:40.48 CS.seek 0ml @11:31:46.49 delay 5 seconds @11:31:51.51 ATV.dial ASV @11:31:52.55 ASV.dial :SPEdelivery @11:31:52.57 AS.seek 0.02ml @11:32:07.16
Flushing lower manifold's waste2b port @11:32:07.17 CSV.dial CSR @11:32:07.79 Pulling 4ml of CSR.mfbkill @11:32:07.80 CSR.select :mfbkill @11:32:07.96 CS.reconfigure CSconfig @11:32:08.02 CS.seek 4ml @11:32:19.23 delay 5 seconds @11:32:24.25 CSR.select :air @11:32:24.40 CS.reconfigure CSfast @11:32:24.47 CS.seek 10ml @11:32:40.88 CSV.dial CTV @11:32:40.91
CTV.dial CSV @11:32:41.46 CBV.dial :waste2b @11:32:42.35 CS.seek 0ml @11:33:08.89 Killing upper manifold @11:33:08.90 CSV.dial CSR @11:33:09.50 Pulling 4ml of CSR.mfbkill @11:33:09.51 CSR.select :mfbkill @11:33:09.68 CS.reconfigure CSconfig @11:33:09.74 CS.seek 4ml @11:33:21.01 delay 5 seconds @11:33:26.03 CSR.select :air @11:33:26.17 CS.reconfigure CSfast @11:33:26.24 CS.seek 10ml @11:33:42.67 CSV.dial CBV @11:33:42.70
CBV.dial CSV @11:33:43.93 CTV.dial :air @11:33:45.49 CS.seek 0ml @11:34:11.98 delay 7 seconds @11:34:19.01 CS.seek 3ml @11:34:27.69 CSV.dial :waste @11:34:28.23 CS.seek 0ml @11:34:37.29 Flushing upper manifold @11:34:37.43 CSV.dial :flush @11:34:37.97 CS.seek 5ml @11:34:52.10 delay 5 seconds @11:34:57.12 CSV.dial :air @11:34:57.70 CS.seek 7ml @11:35:03.76 CSV.dial CBV @11:35:03.79
CBV.dial CSV @11:35:05.03 CTV.dial :air @11:35:05.05 CS.seek 0ml @11:35:09.97 delay 5 seconds @11:35:14.99 ATV.dial :air2 @11:35:16.05 AS.seek 0.52ml @11:35:23.82
delay 7 seconds @11:35:30.85 CS.seek 3ml @11:35:39.50 CSV.dial :waste @11:35:40.06 CS.seek 0ml @11:35:48.75 CSV.dial :flush @11:35:49.28 CS.seek 3ml @11:35:58.04 delay 5 seconds @11:36:03.06 CSV.dial :air @11:36:03.65 CS.seek 5ml @11:36:09.72 CSV.dial :air @11:36:09.75 CS.seek 0ml @11:36:23.42 delay 7 seconds @11:36:30.46 CS.seek 3ml @11:36:39.16 CSV.dial :waste @11:36:40.07 CS.seek 0ml @11:36:48.73 Flushing lower manifold's waste1 port @11:36:48.87 CSV.dial :flush @11:36:49.43 CS.seek 5ml @11:36:56.91 delay 5 seconds @11:37:01.93 ATV.dial ASV @11:37:02.96 ASV.dial :wasteR @11:37:03.34
delay 5 seconds @11:37:03.40 AS.seek 0.3ml @11:37:08.36
CSV.dial :air @11:37:08.95 CS.seek 7ml @11:37:15.07 CSV.dial CTV @11:37:15.10
CTV.dial CSV @11:37:16.73 CBV.dial :waste1 @11:37:17.27 CS.seek 0ml @11:37:36.04 Flushing lower manifold's waste2b port @11:37:36.18 CSV.dial :flush @11:37:37.18 CS.seek 5ml @11:37:47.93 delay 20 seconds @11:37:50.98
delay 5 seconds @11:37:56.00 CSV.dial :air @11:37:56.59 CS.seek 7ml @11:38:02.79 CSV.dial CTV @11:38:02.82
CTV.dial CSV @11:38:03.34 CBV.dial :waste2b @11:38:04.22 CS.seek 0ml @11:38:07.96 AS.seek 0.07ml @11:38:22.98
Garaging FlushPuck in Collection Clamp @11:38:23.08 Elbow.reconfigure TightElbow @11:38:23.15 Elbow.seek CC @11:38:23.39 Elbow.reconfigure LooseElbow @11:38:23.51 Forearm.seek CC @11:38:24.49 Hand.close @11:38:25.20 CC.seek :open @11:38:29.59 Forearm.seek :retract @11:38:30.59 Elbow.reconfigure TightElbow @11:38:30.67 Elbow.seek :garage @11:38:34.22 Elbow.reconfigure LooseElbow @11:38:34.35 Forearm.reconfigure TightForearm @11:38:34.41 Forearm.seek :garage @11:38:35.61 Hand.open @11:38:36.39 Forearm.reconfigure LooseForearm @11:38:36.46 Forearm.seek :retract @11:38:37.48 Starting initialPurge @11:38:37.49 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:38:37.59 Hand.open @11:38:37.89 Elbow.reconfigure TightElbow @11:38:37.95 Elbow.seek FlushPuck::Garage @11:38:38.17 Elbow.reconfigure LooseElbow @11:38:38.29 Forearm.seek :garage @11:38:39.33 Hand.close @11:38:40.19 Forearm.seek :retract @11:38:41.25 Elbow.reconfigure TightElbow @11:38:41.32 Elbow.seek CC @11:38:44.25 Elbow.reconfigure LooseElbow @11:38:44.37 Forearm.reconfigure TightForearm @11:38:44.44 Forearm.seek CC @11:38:45.62 CC.seek :closed @11:38:49.92 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.42 @11:38:50.03 Hand.open @11:38:50.89 Forearm.reconfigure LooseForearm @11:38:50.96 Forearm.seek :retract @11:38:51.92 Flushing the Collection Valve::Manifold @11:38:52.07 CSV.dial CSR @11:38:52.64 Pulling 5ml of CSR.flush @11:38:52.65 CSR.select :flush @11:38:52.82 CS.reconfigure CSconfig @11:38:52.89 CS.seek 5ml @11:38:54.50 delay 20 seconds @11:39:06.73
delay 5 seconds @11:39:11.75 CSR.select :air @11:39:11.93 CS.reconfigure CSfast @11:39:12.00 CS.seek 7.5ml @11:39:14.52 ATV.dial :waste2 @11:39:15.30 AS.reconfigure ASconfig @11:39:15.37 AS.seek :empty @11:39:19.47
CSV.dial :waste @11:39:19.77 Elution @11:39:19.78 ATV.dial :air1 @11:39:20.93 AS.reconfigure ASslow1 @11:39:21.03 AS.seek 0.049ml @11:39:21.06
CS.reconfigure CSconfig @11:39:21.17 CS.seek 0ml @11:39:31.52 delay 3 seconds @11:39:34.54 ATV.dial :ElutionWater @11:39:35.24 AS.seek 0.109ml @11:39:41.36
Purging external Intake @11:39:41.50 CSV.dial :flush @11:39:42.03 CS.reconfigure CSfast @11:39:42.10 CS.seek 10ml @11:39:47.58 delay 3 seconds @11:39:50.60 ATV.dial ASV,via: :air2 @11:39:52.75 ASV.dial :SPEelution @11:39:53.37 AFV.select :carrierSolution @11:39:53.55 AS.reconfigure ASnorm @11:39:53.62 AS.seek 0.759ml @11:40:08.55
delay 5 seconds @11:40:13.57 CSV.dial CBV @11:40:13.61
CBV.dial CSV @11:40:14.55 CTV.dial :intake @11:40:15.44 Intake.select :open @11:40:16.05 CS.seek 0ml @11:40:26.71 delay 3 seconds @11:40:29.73 AFV.select :holdingCoil @11:40:29.88 AS.reconfigure ASslow1 @11:40:29.95 AS.seek 0.474ml @11:40:43.29
Intake.select :close @11:40:43.43 CTV.dial :air @11:40:44.31 Flushing upper manifold @11:40:44.45 CSV.dial :flush @11:40:45.34 CS.seek 5ml @11:40:59.17 delay 5 seconds @11:41:04.19 CSV.dial :air @11:41:04.78 CS.seek 7ml @11:41:10.92 CSV.dial CBV @11:41:10.95
CBV.dial CSV @11:41:12.17 CTV.dial :air @11:41:12.19 CS.seek 0ml @11:41:27.58 delay 5 seconds @11:41:30.98
delay 7 seconds @11:41:32.69 AS.reconfigure ASslow2 @11:41:32.76 AS.seek 0.439ml @11:41:38.01
CS.seek 3ml @11:41:46.66 CSV.dial :waste @11:41:47.22 CS.seek 0ml @11:41:56.18 CSV.dial :flush @11:41:56.72 CS.seek 3ml @11:42:05.39 delay 5 seconds @11:42:08.14 delay 1:00 @11:42:10.41
CSV.dial :air @11:42:11.00 CS.seek 5ml @11:42:17.04 CSV.dial :air @11:42:17.07 CS.seek 0ml @11:42:30.77 delay 7 seconds @11:42:37.81 CS.seek 3ml @11:42:46.35 CSV.dial :waste @11:42:47.25 CS.seek 0ml @11:42:56.08 Garaging FlushPuck in Collection Clamp @11:42:56.18 Elbow.reconfigure TightElbow @11:42:56.24 Elbow.seek CC @11:42:56.49 Elbow.reconfigure LooseElbow @11:42:56.61 Forearm.seek CC @11:42:57.60 Hand.close @11:42:58.33 CC.seek :open @11:43:02.72 Forearm.seek :retract @11:43:03.72 Elbow.reconfigure TightElbow @11:43:03.79 Elbow.seek :garage @11:43:06.98 Elbow.reconfigure LooseElbow @11:43:07.10 Forearm.reconfigure TightForearm @11:43:07.17 Forearm.seek :garage @11:43:08.16 pushing eluate to holding coil @11:43:08.25 AS.seek 0.279ml @11:43:08.35
Hand.open @11:43:09.14 Forearm.reconfigure LooseForearm @11:43:09.21 Forearm.seek :retract @11:43:10.23 Completed [coreNEG] begun 10:30:09 @11:43:10.26 Extending 18tun until completion of MFBPCR @11:45:49.60 delay 30 seconds @11:46:19.63 ATV.dial :waste,via: :air3 @11:46:21.49 AS.reconfigure ASconfig @11:46:21.56 AS.seek :empty @11:46:38.00 Parking Bleach in LysateSource line and SPEcolumn @11:46:38.01 ATV.dial :air3 @11:46:38.85 AS.reconfigure ASslow1 @11:46:38.93 AS.seek 0.029ml @11:46:45.32 ATV.dial :ElutionWater @11:46:45.95 AS.seek 0.059ml @11:46:52.32 ATV.dial :air3 @11:46:52.96 AS.seek 0.069ml @11:46:55.25 delay 2 seconds @11:46:57.27 ATV.dial :bleach @11:46:57.75 AS.seek 0.769ml @11:49:18.80 delay 3 seconds @11:49:21.82 ATV.dial :air3 @11:49:22.40 delay 3 seconds @11:49:25.47 AS.seek 0.794ml @11:49:31.26 delay 3 seconds @11:49:34.28 ATV.dial :bleach @11:49:34.91 AS.seek 0.779ml @11:49:38.21 delay 3 seconds @11:49:41.23 ATV.dial :AIV @11:49:42.51 AS.reconfigure ASconfig @11:49:42.58 AS.seek 0.015ml @11:50:26.81 ATV.dial :waste @11:50:28.35 AS.seek :empty @11:50:29.62 ATV.dial :air3 @11:50:30.32 AS.reconfigure ASslow1 @11:50:30.39 AS.seek 0.009ml @11:50:32.76 delay 2 seconds @11:50:34.78 ATV.dial :bleach @11:50:35.25 AS.seek 0.579ml @11:52:30.15 delay 3 seconds @11:52:33.17 ATV.dial :air3 @11:52:33.74 delay 3 seconds @11:52:36.81 AS.seek 0.604ml @11:52:42.15 delay 3 seconds @11:52:45.17 ATV.dial :bleach @11:52:45.80 AS.seek 0.589ml @11:52:49.08 delay 3 seconds @11:52:52.10 ATV.dial :ASV @11:52:53.14 ASV.dial :wasteR @11:52:53.77 AS.seek 0ml @11:54:52.54 delay 1:00 @11:55:52.57 AFV.select :carrierSolution @11:55:52.67 delay 2 seconds @11:55:54.69 AS.reconfigure ASconfig @11:55:54.76 AS.seek 1ml @11:56:52.63 delay 1 second @11:56:53.65 AFV.select :holdingCoil @11:56:53.75 delay 1 second @11:56:54.77 ATV.dial ASV @11:56:54.78 ASV.dial :wasteR @11:56:54.83 AS.reconfigure ASslow1 @11:56:54.90 AS.seek 0ml @11:57:48.37 Session Begun @11:57:58.62 -> showlog 10 @12:00:16.30 delay 20 seconds @12:00:36.33 ATV.dial :air3 @12:00:37.87 AS.seek 0.59ml @12:02:36.85 delay 3 seconds @12:02:39.87 ATV.dial :ASV @12:02:41.32 ASV.dial :wasteR @12:02:41.42 AS.seek 0.09ml @12:04:22.24 delay 30 seconds @12:04:52.27 ATV.dial :extSrc @12:04:53.07 delay 3 seconds @12:04:56.09 ATV.dial :ElutionWater @12:04:57.24 AS.seek 0.12ml @12:05:03.58 ATV.dial :air3 @12:05:04.24 AS.seek 0.13ml @12:05:06.52 delay 2 seconds @12:05:08.54 ATV.dial :bleach @12:05:09.02 AS.seek 0.23ml @12:05:29.45 delay 3 seconds @12:05:32.47 ATV.dial :air3 @12:05:33.04 delay 3 seconds @12:05:36.11 AS.seek 0.255ml @12:05:41.46 delay 3 seconds @12:05:44.48 ATV.dial :bleach @12:05:45.12 AS.seek 0.24ml @12:05:48.45 delay 3 seconds @12:05:51.47 ATV.dial :ASV @12:05:52.54 ASV.dial :SPEdelivery @12:05:52.94 AFV.select :carrierSolution @12:05:53.08 delay 2 seconds @12:05:55.10 AS.reconfigure ASconfig @12:05:55.19 AS.seek 0.5ml @12:06:10.48 delay 1 second @12:06:11.51 AFV.select :holdingCoil @12:06:11.67 AS.reconfigure ASslow1 @12:06:11.74 AS.seek 0.17ml @12:07:18.39 SPE.hold 2:46:40,95C @12:07:18.44 delay 3:00 @12:10:18.50 AFV.select :carrierSolution @12:10:18.63 delay 2 seconds @12:10:20.65 AS.reconfigure ASconfig @12:10:20.73 AS.seek 1ml @12:11:08.74 delay 1 second @12:11:09.76 AFV.select :holdingCoil @12:11:09.87 delay 1 second @12:11:10.89 ASV.dial :wasteD @12:11:11.99 AS.seek 0ml @12:11:30.85 Session Ended @12:12:09.82 Priming Eluate @12:12:09.83 AFV.select :carrierSolution @12:12:09.97 delay 2 seconds @12:12:12.00 AS.seek 0.5ml @12:12:31.99 Session Ended @12:12:41.06 delay 1 second @12:12:42.08 AFV.select :holdingCoil @12:12:42.18 ATV.dial ASV @12:12:42.19 ASV.dial :SPErecovery @12:12:43.30 Swish #1 of 2 @12:12:43.39 AS.reconfigure ASslow0 @12:12:43.45 AS.seek 0.6ml @12:12:53.87 AS.seek 0.5ml @12:13:04.24 Swish #2 of 2 @12:13:04.33 AS.seek 0.6ml @12:13:14.82 AS.seek 0.5ml @12:13:25.20 delay 3 seconds @12:13:28.27 AS.reconfigure ASslow1 @12:13:28.33 AS.seek 0.684ml @12:14:05.71 delay 3 seconds @12:14:08.73 Eluate primed @12:14:08.74 ATV.dial :waste2 @12:14:09.39 AS.reconfigure ASnorm @12:14:09.45 AS.seek 0ml @12:14:44.25 SPE.coast @12:14:44.34 ASV.dial :SPErecovery @12:14:44.36 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118aug2312h.pcr @12:14:44.38 Building PCR Slug from ASV with MM2 mix, PP4 primer @12:14:44.41 Priming with carrier @12:14:44.43 ATV.dial ARV @12:14:45.43 ARV.dial :carrier @12:14:45.77 AS.seek 0.5ml @12:15:11.33 AFV.select :carrierSolution @12:15:11.46 delay 2 seconds @12:15:13.48 AS.reconfigure ASconfig @12:15:13.55 AS.seek 1ml @12:15:42.59 delay 1 second @12:15:43.61 AFV.select :holdingCoil @12:15:43.71 delay 1 second @12:15:44.73 ATV.dial :waste @12:15:46.25 AS.reconfigure ASnorm @12:15:46.32 AS.seek :empty @12:16:37.11 AFV.select :carrierSolution @12:16:37.23 delay 2 seconds @12:16:39.25 AS.reconfigure ASconfig @12:16:39.32 AS.seek 0.5ml @12:17:08.43 delay 1 second @12:17:09.45 AFV.select :holdingCoil @12:17:09.55 Building Slug @12:17:09.57 ATV.dial :ARV @12:17:11.21 delay 2 seconds @12:17:13.23 ARV.dial :air1 @12:17:13.65 delay 2 seconds @12:17:15.72 AS.reconfigure ASslow2 @12:17:15.78 AS.seek 0.506ml @12:17:22.32 delay 2 seconds @12:17:24.34 ARV.dial :MM2 @12:17:25.86 delay 2 seconds @12:17:27.88 Adding MM2 @12:17:27.97 AS.seek 0.524ml @12:17:46.61 delay 25 seconds @12:18:11.63 ARV.dial :PP4 @12:18:12.03 Adding PP4 @12:18:12.04 delay 2 seconds @12:18:14.12 AS.seek 0.53ml @12:18:20.35 delay 10 seconds @12:18:30.38 ARV.dial :air3 @12:18:30.79 adding ARV air @12:18:30.80 delay 2 seconds @12:18:32.88 AS.seek 0.536ml @12:18:39.28 delay 5 seconds @12:18:44.30 ARV.dial :carrier @12:18:45.59 delay 7 seconds @12:18:52.67 AS.seek 0.594ml @12:19:51.28 delay 5 seconds @12:19:56.30 ATV.dial :ASV @12:19:56.70 delay 2 seconds @12:19:58.77 AS.seek 0.6ml @12:20:04.95 delay 4 seconds @12:20:08.97 ATV.dial ARV @12:20:09.51 delay 2 seconds @12:20:11.53 ARV.dial :carrier @12:20:11.54 delay 2 seconds @12:20:13.62 AS.seek 0.65ml @12:21:04.34 delay 4 seconds @12:21:08.36 ATV.dial PCR @12:21:08.98 ASV.dial :waste1 @12:21:09.79 ARV.dial :air1 @12:21:10.20 Positioning Slug by 0.324ml @12:21:10.29 AS.seek 0.326ml @12:26:36.51 delay 5 seconds @12:26:41.53 ATV.dial ARV @12:26:42.40 ASV.dial :PCRlock @12:26:42.76 delay 5 seconds @12:26:47.78 Running Tt1 Profile60C300 thermocycling @12:26:47.80 PCR.program Profile60C300 @12:26:47.94 Profile60C300 should complete in < 1:45:21 @12:26:48.38 PCR.run 2:16:41.25 @12:28:26.11 Session Begun @12:28:32.14 -> showlog 100 @13:42:15.26 Session Begun @13:42:21.06 -> showlog 10 @13:42:25.44 -> exit @13:42:25.45 Session Ended @13:47:41.81 PCR.off @13:47:43.32 PCR for Tt1 completed -- closed /var/log/esp/Tt118aug2312h.pcr @13:47:43.33 Full MFB Decontamination @13:47:43.37 Decon extraction column and decon AIV @13:47:43.40 SPE.hold 2:46:40,55C @13:47:43.48 ASV.dial :SPEdelivery @13:47:44.20 delay 20 seconds @13:48:04.23 AFV.select :carrierSolution @13:48:04.34 delay 2 seconds @13:48:06.37 AS.reconfigure ASconfig @13:48:06.51 AS.seek 0.5ml @13:48:16.83 delay 1 second @13:48:17.85 AFV.select :holdingCoil @13:48:17.95 ATV.dial :air1 @13:48:20.11 AS.reconfigure ASslow1 @13:48:20.18 AS.seek 0.52ml @13:48:24.49 ATV.dial :ASV @13:48:26.17 ASV.dial :SPErecovery @13:48:26.87 AS.seek 0.62ml @13:48:47.25 ATV.dial :waste @13:48:49.34 AS.reconfigure ASnorm @13:48:49.41 AS.seek :empty @13:49:21.08 AFV.select :holdingCoil @13:49:21.10 ATV.dial :waste @13:49:21.14 AS.reconfigure ASconfig @13:49:21.23 AS.seek 0ml @13:49:21.55 AFV.select :carrierSolution @13:49:21.68 delay 2 seconds @13:49:23.71 AS.seek 0.5ml @13:49:52.76 delay 1 second @13:49:53.78 AFV.select :holdingCoil @13:49:53.88 ATV.dial :air3 @13:49:54.64 AS.reconfigure ASslow1 @13:49:54.70 AS.seek 0.52ml @13:49:59.02 ATV.dial :air3 @13:49:59.12 AS.seek 0.53ml @13:50:01.36 delay 2 seconds @13:50:03.38 ATV.dial :bleach @13:50:03.86 AS.seek 0.71ml @13:50:40.37 delay 3 seconds @13:50:43.39 ATV.dial :air3 @13:50:43.98 delay 3 seconds @13:50:47.05 AS.seek 0.735ml @13:50:52.29 delay 3 seconds @13:50:55.31 ATV.dial :bleach @13:50:55.97 AS.seek 0.72ml @13:50:59.28 delay 3 seconds @13:51:02.30 ATV.dial ASV @13:51:03.39 ASV.dial :SPErecovery @13:51:03.49 AS.seek 0.365ml @13:52:15.16 ATV.dial :waste @13:52:16.98 AS.reconfigure ASnorm @13:52:17.05 AS.seek :empty @13:52:35.86 ATV.dial :air3 @13:52:36.59 AS.reconfigure ASslow1 @13:52:36.66 AS.seek 0.009ml @13:52:39.03 delay 2 seconds @13:52:41.05 ATV.dial :bleach @13:52:41.51 AS.seek 0.579ml @13:54:36.44 delay 3 seconds @13:54:39.46 ATV.dial :air3 @13:54:40.03 delay 3 seconds @13:54:43.10 AS.seek 0.604ml @13:54:48.45 delay 3 seconds @13:54:51.47 ATV.dial :bleach @13:54:52.09 AS.seek 0.589ml @13:54:55.44 delay 3 seconds @13:54:58.46 ATV.dial :ASV @13:54:59.52 ASV.dial :wasteR @13:54:59.91 AS.seek 0ml @13:56:58.67 delay 1:00 @13:57:58.70 ATV.dial ASV @13:57:58.71 AFV.select :carrierSolution @13:57:58.85 delay 2 seconds @13:58:00.87 AS.reconfigure ASconfig @13:58:00.95 AS.seek 1ml @13:58:58.77 delay 1 second @13:58:59.79 AFV.select :holdingCoil @13:58:59.89 delay 1 second @13:59:00.91 ASV.dial :wasteR @13:59:00.93 AS.reconfigure ASslow1 @13:59:01.01 AS.seek 0ml @14:02:22.41 delay 10 seconds @14:02:32.44 ATV.dial :air3 @14:02:33.98 AS.seek 0.01ml @14:02:36.28 delay 2 seconds @14:02:38.30 ATV.dial :bleach @14:02:38.92 AS.seek 0.58ml @14:04:33.92 delay 3 seconds @14:04:36.94 ATV.dial :air3 @14:04:37.50 delay 3 seconds @14:04:41.08 AS.seek 0.605ml @14:04:46.44 delay 3 seconds @14:04:49.46 ATV.dial :bleach @14:04:50.09 AS.seek 0.59ml @14:04:53.38 delay 3 seconds @14:04:56.40 ATV.dial ASV @14:04:57.47 ASV.dial :SPEdelivery @14:04:57.85 AS.seek 0ml @14:06:56.77 delay 1:00 @14:07:56.80 AFV.select :carrierSolution @14:07:56.90 delay 2 seconds @14:07:58.92 AS.reconfigure ASconfig @14:07:58.99 AS.seek 1ml @14:08:56.80 delay 1 second @14:08:57.82 AFV.select :holdingCoil @14:08:57.92 delay 1 second @14:08:58.94 ATV.dial ASV @14:08:58.95 ASV.dial :SPEdelivery @14:08:59.00 AS.reconfigure ASslow1 @14:08:59.07 AS.seek 0ml @14:12:20.47 delay 10 seconds @14:12:30.50 ATV.dial :air2 @14:12:31.35 AS.reconfigure ASslow0 @14:12:31.43 AS.seek 0.8ml @14:13:52.27 delay 3 seconds @14:13:55.29 ATV.dial :ASV @14:13:56.29 ASV.dial :SPEdelivery @14:13:56.31 AS.reconfigure ASslow1 @14:13:56.39 AS.seek 0.03ml @14:16:31.54 delay 20 seconds @14:16:51.57 ATV.dial :waste,via: :air3 @14:16:53.33 AS.reconfigure ASconfig @14:16:53.40 AS.seek :empty @14:16:55.53 SPE.coast @14:16:55.63 Removing Bleach from Mixing Coil @14:16:55.64 AFV.select :carrierSolution @14:16:55.78 delay 2 seconds @14:16:57.81 AS.seek 0.5ml @14:17:26.93 delay 1 second @14:17:27.95 AFV.select :holdingCoil @14:17:28.05 ATV.dial :ASV @14:17:29.76 ASV.dial :SPErecovery @14:17:30.41 AS.reconfigure ASslow1 @14:17:30.48 AS.seek 0.73ml @14:18:17.01 ASV.dial :wasteD @14:18:17.71 AS.reconfigure ASnorm @14:18:17.78 AS.seek 0ml @14:18:54.96 AFV.select :carrierSolution @14:18:55.10 delay 2 seconds @14:18:57.12 AS.reconfigure ASconfig @14:18:57.19 AS.seek 0.5ml @14:19:26.27 delay 1 second @14:19:27.29 AFV.select :holdingCoil @14:19:27.40 ATV.dial ASV @14:19:27.41 ASV.dial :SPErecovery @14:19:28.10 AS.reconfigure ASslow0 @14:19:28.16 AS.seek 0.3ml @14:19:48.59 ATV.dial :air2 @14:19:49.65 AS.reconfigure ASslow1 @14:19:49.73 AS.seek 0.32ml @14:19:54.07 ATV.dial ASV @14:19:55.13 AS.reconfigure ASslow0 @14:19:55.20 AS.seek 0.57ml @14:20:20.68 ASV.dial :wasteD @14:20:21.30 AS.reconfigure ASnorm @14:20:21.37 AS.seek 0ml @14:20:50.48 AFV.select :carrierSolution @14:20:50.62 delay 2 seconds @14:20:52.64 AS.reconfigure ASconfig @14:20:52.71 AS.seek 0.5ml @14:21:21.79 delay 1 second @14:21:22.81 AFV.select :holdingCoil @14:21:22.92 ATV.dial ASV @14:21:22.93 ASV.dial :SPErecovery @14:21:23.61 AS.reconfigure ASslow0 @14:21:23.68 AS.seek 0.3ml @14:21:44.14 ATV.dial :air2 @14:21:45.21 AS.reconfigure ASslow1 @14:21:45.28 AS.seek 0.32ml @14:21:49.63 ATV.dial ASV @14:21:50.69 AS.reconfigure ASslow0 @14:21:50.76 AS.seek 0.57ml @14:22:16.25 ASV.dial :wasteD @14:22:16.87 AS.reconfigure ASnorm @14:22:16.95 AS.seek 0ml @14:22:46.04 ATV.dial :air2 @14:22:47.04 AS.reconfigure ASslow0 @14:22:47.11 AS.seek 0.3ml @14:23:17.61 ATV.dial :ASV @14:23:19.12 ASV.dial :wasteD @14:23:19.14 AS.reconfigure ASconfig @14:23:19.22 AS.seek 0.05ml @14:23:33.90 ATV.dial :waste @14:23:35.90 AS.seek 0ml @14:23:39.11 ASV.dial :SPEdelivery @14:23:40.13 deconLysateINT @14:23:40.14 ATV.dial :air3 @14:23:40.86 AS.reconfigure ASslow1 @14:23:40.93 AS.seek 0.01ml @14:23:43.29 delay 2 seconds @14:23:45.31 ATV.dial :bleach @14:23:45.79 AS.seek 0.96ml @14:26:57.10 delay 3 seconds @14:27:00.12 ATV.dial :air3 @14:27:00.68 delay 3 seconds @14:27:03.75 AS.seek 0.985ml @14:27:09.07 delay 3 seconds @14:27:12.09 ATV.dial :bleach @14:27:12.71 AS.seek 0.97ml @14:27:16.02 delay 3 seconds @14:27:19.04 ATV.dial AIV @14:27:20.32 AIV.dial :coilToMFB @14:27:20.33 AWV.select :waste @14:27:20.37 AFV.select :carrierSolution @14:27:20.51 delay 2 seconds @14:27:22.53 AS.reconfigure ASconfig @14:27:22.61 AS.seek 1ml @14:27:24.68 delay 1 second @14:27:25.70 AFV.select :holdingCoil @14:27:25.80 delay 1 second @14:27:26.82 AS.reconfigure ASslow0 @14:27:26.90 AS.seek :empty @14:29:07.92 AFV.select :carrierSolution @14:29:08.06 delay 2 seconds @14:29:10.08 AS.reconfigure ASconfig @14:29:10.16 AS.seek 1ml @14:30:07.98 delay 1 second @14:30:09.00 AFV.select :holdingCoil @14:30:09.10 delay 1 second @14:30:10.12 AS.reconfigure ASslow0 @14:30:10.20 AS.seek :empty @14:31:51.22 AFV.select :carrierSolution @14:31:51.36 delay 2 seconds @14:31:53.38 AS.reconfigure ASconfig @14:31:53.45 AS.seek 1ml @14:32:51.31 delay 1 second @14:32:52.33 AFV.select :holdingCoil @14:32:52.44 delay 1 second @14:32:53.46 AS.reconfigure ASslow0 @14:32:53.53 AS.seek :empty @14:34:34.58 ATV.dial :air2 @14:34:35.27 AS.reconfigure ASslow1 @14:34:35.34 AS.seek 0.009ml @14:34:37.60 AS.reconfigure ASslow0 @14:34:37.67 AS.seek 1ml @14:36:17.62 ATV.dial AIV @14:36:18.51 AS.seek 0.02ml @14:37:57.39 ATV.dial :waste @14:37:59.06 AS.seek 0ml @14:38:01.39 AWV.select :air @14:38:01.58 Cleaning PCR @14:38:01.59 AFV.select :carrierSolution @14:38:01.73 delay 2 seconds @14:38:03.75 AS.reconfigure ASconfig @14:38:03.83 AS.seek 0.5ml @14:38:32.90 delay 1 second @14:38:33.92 AFV.select :holdingCoil @14:38:34.02 ASV.dial :waste1 @14:38:34.42 ATV.dial :air3 @14:38:35.09 AS.reconfigure ASslow1 @14:38:35.17 AS.seek 0.51ml @14:38:37.49 delay 2 seconds @14:38:39.51 ATV.dial :bleach @14:38:39.96 AS.seek 0.81ml @14:39:40.56 delay 3 seconds @14:39:43.58 ATV.dial :air3 @14:39:44.15 delay 3 seconds @14:39:47.22 AS.seek 0.835ml @14:39:52.53 delay 3 seconds @14:39:55.56 ATV.dial :bleach @14:39:56.18 AS.seek 0.82ml @14:39:59.51 delay 3 seconds @14:40:02.53 ARV.dial :waste @14:40:03.15 delay 3 seconds @14:40:06.17 ATV.dial :ARV @14:40:07.04 AS.reconfigure ASconfig @14:40:07.12 AS.seek 0.47ml @14:40:27.55 AFV.select :carrierSolution @14:40:27.69 delay 2 seconds @14:40:29.72 AS.seek 1ml @14:41:00.98 delay 1 second @14:41:02.00 AFV.select :holdingCoil @14:41:02.10 delay 1 second @14:41:03.12 AS.reconfigure ASfast @14:41:03.19 AS.seek 0ml @14:41:30.59 AFV.select :carrierSolution @14:41:30.73 delay 2 seconds @14:41:32.75 AS.reconfigure ASconfig @14:41:32.83 AS.seek 1ml @14:42:30.62 delay 1 second @14:42:31.64 AFV.select :holdingCoil @14:42:31.75 delay 1 second @14:42:32.77 AS.reconfigure ASfast @14:42:32.84 AS.seek 0ml @14:43:00.22 AFV.select :carrierSolution @14:43:00.36 delay 2 seconds @14:43:02.38 AS.reconfigure ASconfig @14:43:02.45 AS.seek 1ml @14:44:00.25 delay 1 second @14:44:01.27 AFV.select :holdingCoil @14:44:01.38 delay 1 second @14:44:02.40 AS.reconfigure ASfast @14:44:02.48 AS.seek 0ml @14:44:29.87 ARV.dial between carrier and air1 @14:44:30.39 ATV.dial :air3 @14:44:31.68 AS.reconfigure ASslow1 @14:44:31.75 AS.seek 0.01ml @14:44:34.14 delay 2 seconds @14:44:36.16 ATV.dial :bleach @14:44:36.79 AS.seek 0.81ml @14:47:17.97 delay 3 seconds @14:47:20.99 ATV.dial :air3 @14:47:21.56 delay 3 seconds @14:47:24.63 AS.seek 0.835ml @14:47:29.96 delay 3 seconds @14:47:32.98 ATV.dial :bleach @14:47:33.60 AS.seek 0.82ml @14:47:36.92 delay 3 seconds @14:47:39.94 ATV.dial PCR @14:47:40.34 AS.reconfigure ASconfig @14:47:40.41 AS.seek :empty @14:48:27.89 PCR.program :bleachProfile @14:48:27.97 PCR program should complete in < 2:45 @14:48:27.98 PCR.run 10:00 @14:51:35.89 PCR.off @14:51:35.94 AFV.select :carrierSolution @14:51:36.06 delay 2 seconds @14:51:38.09 AS.seek 1ml @14:52:35.98 delay 1 second @14:52:37.00 AFV.select :holdingCoil @14:52:37.10 delay 1 second @14:52:38.12 AS.reconfigure ASfast @14:52:38.19 AS.seek 0ml @14:53:05.59 delay 3 seconds @14:53:08.61 AFV.select :carrierSolution @14:53:08.71 delay 2 seconds @14:53:10.73 AS.reconfigure ASconfig @14:53:10.81 AS.seek 1ml @14:54:08.61 delay 1 second @14:54:09.63 AFV.select :holdingCoil @14:54:09.73 delay 1 second @14:54:10.75 AS.reconfigure ASfast @14:54:10.82 AS.seek 0ml @14:54:38.20 delay 3 seconds @14:54:41.22 AFV.select :carrierSolution @14:54:41.33 delay 2 seconds @14:54:43.35 AS.reconfigure ASconfig @14:54:43.44 AS.seek 1ml @14:55:41.22 delay 1 second @14:55:42.24 AFV.select :holdingCoil @14:55:42.34 delay 1 second @14:55:43.36 AS.reconfigure ASfast @14:55:43.44 AS.seek 0ml @14:56:10.84 delay 3 seconds @14:56:13.86 AFV.select :carrierSolution @14:56:13.96 delay 2 seconds @14:56:15.98 AS.reconfigure ASconfig @14:56:16.05 AS.seek 1ml @14:57:13.84 delay 1 second @14:57:14.86 AFV.select :holdingCoil @14:57:14.96 delay 1 second @14:57:15.98 AS.reconfigure ASfast @14:57:16.06 AS.seek 0ml @14:57:43.44 delay 3 seconds @14:57:46.46 AFV.select :carrierSolution @14:57:46.56 delay 2 seconds @14:57:48.58 AS.reconfigure ASconfig @14:57:48.66 AS.seek 1ml @14:58:46.45 delay 1 second @14:58:47.47 AFV.select :holdingCoil @14:58:47.57 delay 1 second @14:58:48.59 AS.reconfigure ASnorm @14:58:48.66 AS.seek 0ml @14:59:39.40 AFV.select :carrierSolution @14:59:39.53 delay 2 seconds @14:59:41.55 AS.reconfigure ASconfig @14:59:41.63 AS.seek 1ml @15:00:39.42 delay 1 second @15:00:40.44 AFV.select :holdingCoil @15:00:40.54 delay 1 second @15:00:41.56 ATV.dial :waste @15:00:43.30 AS.reconfigure ASnorm @15:00:43.38 AS.seek 0.5ml @15:01:08.94 ATV.dial :waste2 @15:01:10.25 AS.seek 0ml @15:01:35.87
Asleep after completing 18tun assays @15:01:35.88 Mission runtime was 4:32:14.273505 @15:01:35.91 GoodNews.email "{1 => 2, 2 => 0}\nCan@14:59:20, 15.9C, 48% humidity, 14.5psia, 12.065V, 0.418A, 0.418A avg, 5.04W",Subject: "Asleep after completing 18tun assays" @15:01:44.13 Session Ended @15:01:44.18 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @12:38:39.83CEST25-Aug-18 LoadError in MAIN_scheduler -- no such file to load -- i2c/gateway in i2c/gateway @12:38:39.86 LoadError in MAIN_scheduler -- no such file to load -- base in base @12:38:41.42
ESP2 v3.30 I2C Gateway v3.06 for core bus @12:38:43.27 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @12:38:43.42 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @12:38:48.53 Polling Can every 10:00 @12:38:48.54 NameError in MAIN -- uninitialized constant ESP::Power @12:38:48.57 NameError in MAIN -- uninitialized constant ESP::CmdServer @12:38:48.60 NoMethodError in MAIN -- undefined method `stop!' for ESP:Module @12:38:48.61 BadNews.email "uninitialized constant ESP::CmdServer",Subject: "NameError in MAIN" @12:38:57.20 TypeError in MAIN -- can't convert nil into String @11:53:52.11CEST26-Aug-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @11:53:54.03 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @11:53:54.55 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @11:54:01.39 Polling Can every 10:00 @11:54:03.14 SPE.coast @11:54:03.18 SPE.configure SPEconfig @11:54:03.26 AS.configure ASconfig @11:54:03.35 SS.reconfigure SSconfig @11:54:03.39 SS.hold @11:54:03.44 SS.configure SSconfig @11:54:03.53 CC.reconfigure CCconfig @11:54:03.57 CC.hold @11:54:03.64 CC.configure CCconfig @11:54:03.72 CS.reconfigure CSconfig @11:54:03.76 CS.hold @11:54:03.82 CS.configure CSconfig @11:54:03.90 PC.reconfigure PCconfig @11:54:03.93 PC.hold @11:54:04.32 PC.configure PCconfig @11:54:04.40 PS.configure PSconfig @11:54:04.51 Forearm.reconfigure LooseForearm @11:54:04.54 Forearm.hold @11:54:04.59 Forearm.configure LooseForearm @11:54:04.67 Elbow.reconfigure LooseElbow @11:54:04.71 Elbow.hold @11:54:04.77 Elbow.configure LooseElbow @11:54:04.86 SC.reconfigure SCloose @11:54:04.90 SC.hold @11:54:04.95 SC.configure SCloose @11:54:05.04 SE.reconfigure SEconfig @11:54:05.08 SE.hold @11:54:05.14 SE.configure SEconfig @11:54:05.17 CH.coast @11:54:05.22 CH.configure CHconfig @11:54:05.24 PH.coast @11:54:05.29 PH.configure PHconfig @11:54:05.33 PCR.off @11:54:05.36 PCR.pidtemp= 50,0.1,0,300,25,25 @11:54:05.39 PCR.caltemp= 2.7073,0.0373,0 @11:54:05.42 All dwarf controllers running firmware version 3.75 @11:54:05.53 Storage::Warning in MAIN -- Uncleanly Exited @11:54:05.56 Loading /home/esp/esp2/mission/18tun.rb mission @11:54:05.62 Preparing to sleep until +0:00:15 @11:54:05.65 GoodNews.email "Starting 18tun w/pucks in tube 2\n{}\nCan@11:54:01, 16.4C, 43% humidity, 14.5psia, 12.001V, 0.657A, 0.657A avg, 7.89W",Subject: "18tun begun" @11:54:05.71 SS.coast @11:54:05.73
Asleep til +0:00:15 @11:54:05.77 Continuing at 11:54 @11:54:05.79 CC.coast @11:54:05.87 CS.coast @11:54:05.90 PC.coast @11:54:05.93 Forearm.coast @11:54:05.96 Elbow.coast @11:54:05.99 SC.coast @11:54:06.02 SE.coast @11:54:06.42 Gate.power :core,:OFF @11:54:06.44 Gate.power :raw,:OFF @11:54:06.45 Shutdown @11:54:13.51 GoodNews.email "Then, in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:106\n{}\nCan@11:54:05, 16.4C, 43% humidity, 14.6psia, 12.001V, 0.657A, 0.657A avg, 7.89W",Subject: "Asleep until 11:54" @11:54:20.59
Powering core bus @11:54:25.43 Gate.power :raw,:ON @11:54:26.06 SPE.coast @11:54:26.11 SPE.configure SPEconfig @11:54:26.19 AS.configure ASconfig @11:54:26.27 SS.configure SSconfig @11:54:26.36 CC.configure CCconfig @11:54:26.44 CS.configure CSconfig @11:54:26.52 PC.configure PCconfig @11:54:26.60 PS.configure PSconfig @11:54:26.71 Forearm.configure LooseForearm @11:54:26.78 Elbow.configure LooseElbow @11:54:26.90 SC.configure SCloose @11:54:27.02 SE.configure SEconfig @11:54:27.06 CH.coast @11:54:27.10 CH.configure CHconfig @11:54:27.13 PH.coast @11:54:27.18 PH.configure PHconfig @11:54:27.22 PCR.off @11:54:27.24 PCR.pidtemp= 50,0.1,0,300,25,25 @11:54:27.27 PCR.caltemp= 2.7073,0.0373,0 @11:54:27.30 All dwarf controllers running firmware version 3.75 @11:54:27.40 Valve.reset @11:54:27.55 PCR.off @11:54:27.58 PCR.pidtemp= 50,0.1,0,300,25,25 @11:54:27.61 PCR.caltemp= 2.7073,0.0373,0 @11:54:27.82 PSV.dial :air @11:54:27.85 Intake.select :close @11:54:27.87 Exhaust.select :close @11:54:27.90 CTV.dial :air @11:54:27.99 CSV.dial :waste1 @11:54:28.13
Forearm.reconfigure LooseForearm @11:54:28.15 PS.reconfigure PSconfig @11:54:28.22
Forearm.home.seek :retract @11:54:28.28 PS.home.seek 0ml @11:54:28.33 SSV.dial :spit,avoiding: :exhaust @11:54:28.82 CS.reconfigure CSfast @11:54:28.84 SS.reconfigure SSconfig @11:54:28.96 CS.home.seek 0ml @11:54:28.99 SS.home.seek 0ml @11:54:29.09 Hand.close @11:54:29.13
Elbow.jog 1500 @11:54:29.64 Elbow.reconfigure LooseElbow @11:54:29.68 Elbow.home.seek :clear @11:54:34.41 SE.reconfigure SEconfig @11:54:34.45 SE.home.seek :down @11:54:35.05 SC.reconfigure SCsloppy @11:54:35.09 SC.home.seek :initial @11:54:37.57 PS.coast @11:54:37.84 Session Begun @11:54:39.13
Forearm.seek :retract @11:54:39.23 Elbow.seek :hammer @11:54:40.38 Forearm.reconfigure TightForearm @11:54:40.45 Forearm.seek 5 + 350 counts @11:54:41.50 Forearm.seek :retract @11:54:42.66 PC.reconfigure PCconfig @11:54:42.68
CC.reconfigure CCconfig @11:54:42.78 PC.home.seek :open @11:54:42.80
CC.home.seek :open @11:54:46.13 Phase [coreNEG] @11:54:46.14 GoodNews.email "At 11:54:46 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:106\n{}\nCan@11:54:46, 16.6C, 42% humidity, 14.6psia, 11.982V, 0.758A, 0.758A avg, 9.08W",Subject: "Started 18tun phase coreNEG" @11:54:46.18CEST26-Aug-18
==================> STARTED coreNEG (phase #1) <=== @11:54:46.20 Starting initialPurge @11:54:46.22 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:54:46.32 Hand.open @11:54:47.25 Elbow.reconfigure TightElbow @11:54:47.31 Elbow.seek FlushPuck::Garage @11:54:49.29 Elbow.reconfigure LooseElbow @11:54:49.40 Forearm.reconfigure LooseForearm @11:54:49.46 Forearm.seek :garage @11:54:50.47 Hand.close @11:54:51.37 Forearm.seek :retract @11:54:52.44 Elbow.reconfigure TightElbow @11:54:52.51 Elbow.seek CC @11:54:55.46 Elbow.reconfigure LooseElbow @11:54:55.57 Forearm.reconfigure TightForearm @11:54:55.63 Forearm.seek CC @11:54:56.82 CC.seek :closed @11:55:01.18 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.27 @11:55:01.26 Hand.open @11:55:02.06 Forearm.reconfigure LooseForearm @11:55:02.12 Forearm.seek :retract @11:55:03.10 Flushing the Collection Valve::Manifold @11:55:03.23 CSV.dial CSR @11:55:04.80 Pulling 5ml of CSR.flush @11:55:04.81 CSR.select :flush @11:55:04.97 CS.reconfigure CSconfig @11:55:05.03 CS.seek 5ml @11:55:19.00 delay 5 seconds @11:55:24.02 CSR.select :air @11:55:24.20 CS.reconfigure CSfast @11:55:24.26 CS.seek 7.5ml @11:55:31.72 CSV.dial :waste @11:55:33.24 CS.reconfigure CSconfig @11:55:33.31 CS.seek 0ml @11:55:53.38 Purging external Intake @11:55:53.51 CSV.dial :flush @11:55:54.02 CS.reconfigure CSfast @11:55:54.08 CS.seek 10ml @11:56:20.60 delay 5 seconds @11:56:25.62 CSV.dial CBV @11:56:25.66
CBV.dial CSV @11:56:26.57 CTV.dial :intake @11:56:27.44 Intake.select :open @11:56:27.56 CS.seek 0ml @11:56:54.85 Intake.select :close @11:56:54.97 CTV.dial :air @11:56:55.85 Flushing upper manifold @11:56:55.98 CSV.dial :flush @11:56:56.84 CS.seek 5ml @11:57:10.94 delay 5 seconds @11:57:15.96 CSV.dial :air @11:57:16.55 CS.seek 7ml @11:57:22.66 CSV.dial CBV @11:57:22.69
CBV.dial CSV @11:57:23.89 CTV.dial :air @11:57:23.91 CS.seek 0ml @11:57:42.85 delay 7 seconds @11:57:49.88 CS.seek 3ml @11:57:58.50 CSV.dial :waste @11:57:59.05 CS.seek 0ml @11:58:07.85 CSV.dial :flush @11:58:08.76 CS.seek 3ml @11:58:17.47 delay 5 seconds @11:58:22.49 CSV.dial :air @11:58:23.06 CS.seek 5ml @11:58:29.10 CSV.dial :air @11:58:29.12 CS.seek 0ml @11:58:42.85 delay 7 seconds @11:58:49.88 CS.seek 3ml @11:58:58.47 CSV.dial :waste @11:58:59.34 CS.seek 0ml @11:59:07.94 Garaging FlushPuck in Collection Clamp @11:59:08.03 Elbow.reconfigure TightElbow @11:59:08.08 Elbow.seek CC @11:59:08.34 Elbow.reconfigure LooseElbow @11:59:08.45 Forearm.seek CC @11:59:09.46 Hand.close @11:59:10.24 CC.seek :open @11:59:14.61 Forearm.seek :retract @11:59:15.62 Elbow.reconfigure TightElbow @11:59:15.68 Elbow.seek :garage @11:59:18.81 Elbow.reconfigure LooseElbow @11:59:18.92 Forearm.reconfigure TightForearm @11:59:18.97 Forearm.seek :garage @11:59:20.16 Hand.open @11:59:20.93 Forearm.reconfigure LooseForearm @11:59:20.98 Forearm.seek :retract @11:59:22.09 Starting mfb @11:59:22.10 pcrBAC @11:59:22.13 BACfans.email "Started mfb in tube 2 at 11:59:22CEST26-Aug-18\n{}\nCan@11:54:46, 16.6C, 42% humidity, 14.6psia, 11.982V, 0.758A, 0.758A avg, 9.08W",Subject: "pcrBAC" @11:59:22.19
Loading sh1 puck from tube 2 into Collection Clamp @11:59:22.34 Readying the MFB @11:59:22.41 Homing MFB Syringe @11:59:22.43 AFV.select :holdingCoil @11:59:22.65 ATV.dial :air2 @11:59:22.78
SC.reconfigure SCloose @11:59:23.01 Elbow.reconfigure TightElbow @11:59:23.07 Elbow.seek 2 @11:59:23.17 AS.reconfigure ASconfig @11:59:23.24 AS.home.seek :home @11:59:25.33
Forearm.reconfigure TightForearm @11:59:25.39 Forearm.seek 2 + 700 counts @11:59:26.47 SE.reconfigure SEpick @11:59:26.56 SE.seek beamBreak @11:59:28.74 AFV.select :carrierSolution @11:59:28.86 delay 2 seconds @11:59:29.76
SE.configure SEconfig @11:59:29.93 Elbow.seek 2 @11:59:30.28 Forearm.seek 2 + 300 counts @11:59:30.62 Forearm.reconfigure LooseForearm @11:59:30.68 Hand.close @11:59:30.90 AS.seek 1ml @11:59:31.50 SE.seek :down @11:59:31.72
Forearm.seek :retract @11:59:32.68 Elbow.seek CC @11:59:34.28 SE.coast @11:59:34.84
Elbow.reconfigure LooseElbow @11:59:34.97 Forearm.reconfigure TightForearm @11:59:35.03 Forearm.seek CC @11:59:36.33 CC.seek :closed @11:59:40.55 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.41 @11:59:40.56 Hand.open @11:59:41.44 Forearm.reconfigure LooseForearm @11:59:41.49 Forearm.seek :retract @11:59:42.49 SC.coast @11:59:42.54 skipping prime and sample collection @11:59:42.55 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @11:59:42.57 Intake.select :close @11:59:42.67 CSV.dial :waste @11:59:42.75 CS.seek 0ml @11:59:42.87
Calibrating pressure and temperature sensors @11:59:42.88 CTV.dial :air @11:59:42.91 SSV.dial :spit,avoiding: :exhaust @11:59:42.94 SS.reconfigure SSfast @11:59:43.03 CSV.dial :air @11:59:43.06
SS.seek 5ml @11:59:44.05 CS.seek 10ml @11:59:45.97 SSV.dial CBV @11:59:46.00
CBV.dial SSV @11:59:47.55 delay 3.8 seconds @11:59:51.47 SSV.dial :spit @11:59:52.98 SS.seek 0ml @11:59:55.74 SSV.dial CBV @11:59:57.25 SSV.dial CBV,avoiding: :exhaust @11:59:57.29
CBV.dial SSV @11:59:57.30 CTV.dial :air @11:59:57.33 delay 15 seconds @12:00:12.36 SSV.dial :spit,avoiding: :exhaust @12:00:13.86 CTV.dial CSV,avoiding: :intake @12:00:13.90
SS.reconfigure SSexhale @12:00:14.00 SS.seek 0ml @12:00:14.09 SSV.dial CBV,avoiding: :exhaust @12:00:15.66 CBV.dial between SSV and waste2 @12:00:16.09 CTV.dial CSV,avoiding: :air @12:00:16.12
CSV.dial CTV @12:00:16.66 CS.reconfigure CSpressurize @12:00:16.73 CS.seek 0ml @12:00:23.80 delay 1 second @12:00:24.82 AFV.select :holdingCoil @12:00:24.92 delay 1 second @12:00:25.94 ATV.dial :waste @12:00:27.02 AS.reconfigure ASnorm @12:00:27.08 AS.home.seek :empty @12:00:42.85
CSV.dial :air @12:00:43.38 CS.reconfigure CSfast @12:00:43.45 CS.seek 10ml @12:01:09.81 CSV.dial between air and CTV @12:01:10.19 CS.seek 3.416ml @12:01:19.66 ATV.dial :waste @12:01:19.67 ARV.dial between carrier and air1 @12:01:19.77 ASV.dial :waste1 @12:01:27.85 CTV.dial CSV,avoiding: :air @12:01:27.87
CSV.dial CTV @12:01:28.24 CS.reconfigure CSpressurize @12:01:28.31 CS.seek 0ml @12:01:37.76 CSV.dial :air @12:01:38.29 CS.reconfigure CSfast @12:01:38.36 CS.seek 10ml @12:02:04.62 CSV.dial between air and CTV @12:02:05.42 CS.seek 2.154ml @12:02:26.54 CTV.dial CSV,avoiding: :air @12:02:26.57
CSV.dial CTV @12:02:26.93 CS.reconfigure CSpressurize @12:02:27.00 CS.seek 0ml @12:02:29.03 BubblePoint: Intake @69.8psia, Outlet @14.8psia @12:02:29.04 CBV.dial SSV @12:02:29.41 SS.reconfigure SSconfig @12:02:29.47 SS.seek 10ml @12:02:35.60 delay 15 seconds @12:02:50.72 Evacuated: Intake @28.4psia, Outlet @28.7psia @12:02:50.73 SSV.dial :spit,avoiding: :exhaust @12:02:52.25 SS.reconfigure SSexhale @12:02:52.32 SS.seek 0ml @12:02:57.40 SS.reconfigure SSfast @12:02:57.46 SS.seek 5ml @12:03:00.49 SSV.dial CBV,avoiding: :exhaust @12:03:00.52
CBV.dial SSV @12:03:02.04 CTV.dial :air,avoiding: :intake @12:03:03.59 CSV.dial CTV @12:03:03.63
SS.seek 10ml @12:03:04.63 CSV.dial :air @12:03:05.15 CS.reconfigure CSfast @12:03:05.21 CS.seek 0ml @12:03:06.60
SSV.dial :spit,avoiding: :exhaust @12:03:08.11 SS.reconfigure SSexhale @12:03:08.18 SS.seek 0ml @12:03:10.23 CS.coast @12:03:13.47
SS.reconfigure SSfast @12:03:13.54 SS.seek 5ml @12:03:16.36 SSV.dial CBV,avoiding: :exhaust @12:03:16.39
CBV.dial SSV @12:03:18.92 SSV.dial :spit,avoiding: :exhaust @12:03:20.44 SS.reconfigure SSconfig @12:03:20.51 SS.seek 0ml @12:03:23.56 SS.coast @12:03:23.61 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @12:03:23.62 Intake.select :close @12:03:23.71 CSV.dial :waste @12:03:23.83 SSV.dial CBV,avoiding: :exhaust @12:03:23.87
CBV.dial SSV @12:03:24.61 CS.seek 0ml @12:03:24.70 CSV.dial :air @12:03:25.35
CTV.dial :air @12:03:25.36 delay 15 seconds @12:03:25.55 CS.seek 10ml @12:03:40.39
SSV.dial :spit,avoiding: :exhaust @12:03:41.91 CTV.dial CSV,avoiding: :intake @12:03:41.95
SS.reconfigure SSexhale @12:03:42.06 SS.seek 0ml @12:03:42.14 SSV.dial CBV,avoiding: :exhaust @12:03:52.06 CBV.dial between SSV and waste2 @12:03:52.49 CTV.dial CSV,avoiding: :air @12:03:52.52
CSV.dial CTV @12:03:53.05 CS.reconfigure CSpressurize @12:03:53.12 CS.seek 0ml @12:04:19.35 CSV.dial :air @12:04:19.88 CS.reconfigure CSfast @12:04:19.94 CS.seek 10ml @12:04:46.37 CSV.dial between air and CTV @12:04:46.74 CS.seek 3.393ml @12:05:04.38 CTV.dial CSV,avoiding: :air @12:05:04.41
CSV.dial CTV @12:05:04.76 CS.reconfigure CSpressurize @12:05:04.83 CS.seek 0ml @12:05:14.23 CSV.dial :air @12:05:14.76 CS.reconfigure CSfast @12:05:14.83 CS.seek 10ml @12:05:41.06 CSV.dial between air and CTV @12:05:41.86 CS.seek 2.145ml @12:06:02.70 CTV.dial CSV,avoiding: :air @12:06:02.73
CSV.dial CTV @12:06:03.10 CS.reconfigure CSpressurize @12:06:03.16 CS.seek 0ml @12:06:05.22 BubblePoint: Intake @70.4psia, Outlet @15.1psia @12:06:05.23 CBV.dial SSV @12:06:05.60 SS.reconfigure SSconfig @12:06:05.66 SS.seek 10ml @12:06:11.75 delay 15 seconds @12:06:26.87 Evacuated: Intake @28.5psia, Outlet @28.9psia @12:06:26.88 SSV.dial :spit,avoiding: :exhaust @12:06:28.39 SS.reconfigure SSexhale @12:06:28.46 SS.seek 0ml @12:06:33.41 SS.reconfigure SSfast @12:06:33.47 SS.seek 5ml @12:06:36.27 SSV.dial CBV,avoiding: :exhaust @12:06:36.30
CBV.dial SSV @12:06:37.81 CTV.dial :air,avoiding: :intake @12:06:39.35 CSV.dial CTV @12:06:39.40
SS.seek 10ml @12:06:40.38 CSV.dial :air @12:06:40.90 CS.reconfigure CSfast @12:06:40.96 CS.seek 0ml @12:06:42.36
SSV.dial :spit,avoiding: :exhaust @12:06:43.86 SS.reconfigure SSexhale @12:06:43.93 SS.seek 0ml @12:06:45.95 CS.coast @12:06:48.97
SS.reconfigure SSfast @12:06:49.03 SS.seek 5ml @12:06:52.08 SSV.dial CBV,avoiding: :exhaust @12:06:52.11
CBV.dial SSV @12:06:54.64 SSV.dial :spit,avoiding: :exhaust @12:06:56.16 SS.reconfigure SSconfig @12:06:56.22 SS.seek 0ml @12:06:59.26 SS.coast @12:06:59.31 Lysing: Adding 1.5ml lysis @12:06:59.33 CBV.dial between CSV and waste1,avoiding: :DBV @12:07:01.04 CTV.dial CSV @12:07:02.57 CSV.dial CSR @12:07:03.48 Pulling 1ml of CSR.lysis @12:07:03.49 CSR.select :lysis @12:07:03.62 CS.reconfigure CSconfig @12:07:03.68 CS.seek 1ml @12:07:07.29 delay 5 seconds @12:07:12.31 CSR.select :air @12:07:12.48 CS.seek 6ml @12:07:26.39 delay 2 seconds @12:07:28.41 CSV.dial :waste1 @12:07:29.90 CS.reconfigure CSfast @12:07:29.96 CS.seek :empty @12:07:45.95 CSV.dial CSR @12:07:47.48 Pulling 1.5ml of CSR.lysis @12:07:47.49 CSR.select :lysis @12:07:47.64 CS.reconfigure CSconfig @12:07:47.70 CS.seek 1.5ml @12:07:52.64 delay 5 seconds @12:07:57.66 CSR.select :air @12:07:57.83 CS.seek 4.5ml @12:08:06.70 delay 2 seconds @12:08:08.72 CSV.dial CTV @12:08:09.23 CS.seek 1.275ml @12:08:18.49 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @12:08:18.51 CH.seek 85C,8:00 @12:12:01.42 CH.hold 5:00,85C @12:17:01.58 Collection Puck Heater at 84.7C after lysing @12:17:01.60 CS.seek 0ml @12:17:05.86 CTV.dial between spare and air,avoiding: :intake @12:17:07.06 CSV.dial :air @12:17:07.57 CS.seek 5ml @12:17:21.36 CSV.dial CTV @12:17:22.00 delay 2 seconds @12:17:24.02 CSV.dial :air @12:17:24.52 delay 2 seconds @12:17:26.54 CSV.dial CTV @12:17:27.07 delay 2 seconds @12:17:29.09 CSV.dial :air @12:17:29.63 delay 2 seconds @12:17:31.66 CS.seek 0ml @12:17:45.52 Recovering Lysate into Collection syringe @12:17:45.53 CSV.dial CBV @12:17:46.72 CBV.dial CSV @12:17:47.14 CS.seek 5ml @12:18:00.93 delay 5 seconds @12:18:05.95 CTV.dial :air @12:18:06.55 CS.seek 6.999ml @12:18:12.64 delay 5 seconds @12:18:17.66 CSV.dial :air2 @12:18:18.16 CS.seek 10ml @12:18:26.83 CSV.dial between air and CTV,avoiding: CBV @12:18:28.14 Lysing Complete @12:18:28.23 Storing sh1 puck in Collection Clamp to tube 1 @12:18:28.40 SC.reconfigure SCtight @12:18:28.46 SC.seek 1 @12:18:30.96 SC.reconfigure SCloose @12:18:31.24 Elbow.reconfigure TightElbow @12:18:31.31 Elbow.seek 1 @12:18:33.29 Forearm.reconfigure TightForearm @12:18:33.35 Forearm.seek 1 + 700 counts @12:18:34.40 SE.reconfigure SEpick @12:18:34.49 SE.seek beamBreak @12:18:37.49 SE.configure SEconfig @12:18:37.56 SE.seek :place @12:18:38.05 Forearm.reconfigure LooseForearm @12:18:38.11 Forearm.seek :retract @12:18:39.06 Elbow.seek CC @12:18:40.73 Elbow.reconfigure LooseElbow @12:18:40.84 Forearm.seek CC @12:18:41.84 Hand.close @12:18:42.64 CC.seek :open @12:18:47.11 Forearm.seek :retract @12:18:48.09 Elbow.reconfigure TightElbow @12:18:48.15 Elbow.seek 1 @12:18:50.07 Elbow.reconfigure LimpElbow @12:18:50.13 Forearm.seek 1 @12:18:51.18 Elbow.hold @12:18:51.26 Elbow.reconfigure TightElbow @12:18:51.32 Hand.open @12:18:52.13 Forearm.force -20 @12:18:52.18 Forearm.seek 1 + 333 counts @12:18:52.47 Forearm.reconfigure TightForearm @12:18:52.54 Forearm.seek 1 + 700 counts @12:18:52.90 SE.seek :down @12:18:54.04
Forearm.reconfigure LooseForearm @12:18:54.10 Forearm.seek :retract @12:18:55.70 SE.coast @12:18:55.75
SC.coast @12:18:55.81 Tranferring lysate to PRV @12:18:55.82 CSV.dial :air @12:18:56.19 CS.seek 10ml @12:18:56.27 CSV.dial between air and CTV @12:18:56.63 AIV.dial :bypass @12:18:56.72 Beginning samplePassC @12:18:56.73 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:18:56.82 Hand.open @12:18:57.26 Elbow.seek FlushPuck::Garage @12:18:59.25 Elbow.reconfigure LooseElbow @12:18:59.37 Forearm.seek :garage @12:19:00.39 Hand.close @12:19:01.25 Forearm.seek :retract @12:19:02.29 Elbow.reconfigure TightElbow @12:19:02.36 Elbow.seek CC @12:19:05.20 Elbow.reconfigure LooseElbow @12:19:05.31 Forearm.reconfigure TightForearm @12:19:05.37 Forearm.seek CC @12:19:07.14 CC.seek :closed @12:19:11.44 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.44 @12:19:11.53 Hand.open @12:19:12.33 Forearm.reconfigure LooseForearm @12:19:12.38 Forearm.seek :retract @12:19:13.32 AWV.select :air @12:19:13.33 AIV.dial :bypass @12:19:13.35 PSV.dial PSR @12:19:14.92 Pulling 1.5ml of PSR.flush PSR.select :flush @12:19:15.07 PS.seek 1.5ml @12:19:31.50 delay 5 seconds @12:19:36.52 PSV.dial :waste1 @12:19:37.03 PS.seek 0ml @12:19:53.50 PSV.dial PSR @12:19:54.02 PSR.select :air @12:19:54.15 PS.seek 2.5ml @12:20:21.00 PRV.dial CBV @12:20:21.05
CBV.dial PRV @12:20:21.94 PSV.dial :mixloop @12:20:23.11 CTV.dial :air,avoiding: :intake @12:20:23.12 PTV.dial :PRVmixing @12:20:23.22 PS.seek 0ml @12:20:50.10 PSV.dial :air @12:20:50.61 PS.seek 2.5ml @12:21:17.50 PSV.dial :mixloop @12:21:18.02 PS.seek 0ml @12:21:44.88 PSV.dial :air @12:21:45.39 PS.seek 2.5ml @12:22:12.35 PSV.dial :mixloop @12:22:12.86 PS.seek 0ml @12:22:39.79 Garaging FlushPuck in Collection Clamp @12:22:39.88 Elbow.reconfigure TightElbow @12:22:39.94 Elbow.seek CC @12:22:40.14 Elbow.reconfigure LooseElbow @12:22:40.25 Forearm.seek CC @12:22:41.23 Hand.close @12:22:42.02 CC.seek :open @12:22:46.41 Forearm.seek :retract @12:22:47.41 Elbow.reconfigure TightElbow @12:22:47.47 Elbow.seek :garage @12:22:50.74 Elbow.reconfigure LooseElbow @12:22:50.85 Forearm.reconfigure TightForearm @12:22:50.91 Forearm.seek :garage @12:22:52.08 Hand.open @12:22:52.95 Forearm.reconfigure LooseForearm @12:22:53.01 Forearm.seek :retract @12:22:54.08 Connecting PS to CS to transfer sample @12:22:54.09 CBV.dial AIV @12:22:54.10 AIV.dial :bypass @12:22:54.12 PSV.dial :mixloop @12:22:54.14 PTV.dial :PRVmixing @12:22:54.17 PRV.dial AIV @12:22:54.19 CTV.dial :air,avoiding: :intake @12:22:54.22 Loading lyfil puck from tube 2 into Collection Clamp @12:22:54.90 SC.reconfigure SCtight @12:22:54.96 SC.seek 2 @12:22:57.19 SC.reconfigure SCloose @12:22:57.40 Elbow.reconfigure TightElbow @12:22:57.46 Elbow.seek 2 @12:22:59.54 Forearm.reconfigure TightForearm @12:22:59.60 Forearm.seek 2 + 700 counts @12:23:00.68 SE.reconfigure SEpick @12:23:00.77 SE.seek beamBreak @12:23:03.96 SE.configure SEconfig @12:23:04.12 Elbow.seek 2 @12:23:04.31 Forearm.seek 2 + 300 counts @12:23:04.58 Forearm.reconfigure LooseForearm @12:23:04.64 Hand.close @12:23:05.40 SE.seek :down @12:23:05.61
Forearm.seek :retract @12:23:06.59 Elbow.seek CC @12:23:08.25 Elbow.reconfigure LooseElbow @12:23:08.37 SE.coast @12:23:08.39
Forearm.reconfigure TightForearm @12:23:08.48 Forearm.seek CC @12:23:09.70 CC.seek :closed @12:23:13.99 Collection Clamp onSpring/offSpring for 6/30 samples, on/off torque 2.50 @12:23:14.00 Hand.open @12:23:14.82 Forearm.reconfigure LooseForearm @12:23:14.88 Forearm.seek :retract @12:23:15.91 SC.coast @12:23:15.95 CTV.dial CSV,avoiding: :intake @12:23:17.49 CSV.dial CTV @12:23:17.85 CS.seek 5ml @12:23:31.59 delay 1:20 @12:24:51.62 CS.seek 2.5ml @12:24:59.09 delay 40 seconds @12:25:39.12 CS.seek 0ml @12:25:46.46 delay 1:00 @12:26:46.48 PSV.dial :air @12:26:46.99 PS.seek 2.5ml @12:27:13.94 PSV.dial :mixloop @12:27:14.46 delay 3 seconds @12:27:17.48 PSV.dial :air @12:27:17.98 delay 2 seconds @12:27:20.00 PSV.dial :mixloop @12:27:20.49 delay 3 seconds @12:27:23.51 PSV.dial :waste1 @12:27:25.02 PS.seek :empty @12:27:51.91 CBV.dial :waste1 @12:27:52.43 delay 5 seconds @12:27:57.45 CBV.dial AIV @12:27:58.00 delay 10 seconds @12:28:08.03 CBV.dial :waste1 @12:28:08.56 delay 10 seconds @12:28:18.59 CBV.dial AIV @12:28:19.12 delay 10 seconds @12:28:29.15 CTV.dial CSV,avoiding: :intake @12:28:29.16 CBV.dial between AIV and waste1 @12:28:29.56 CSV.dial :air @12:28:30.09 CS.seek 10ml @12:28:57.06 CSV.dial CTV @12:28:57.57 CS.seek 0ml @12:29:24.18 CBV.dial :waste1 @12:29:24.53 delay 10 seconds @12:29:34.56 CTV.dial :air,avoiding: :intake @12:29:36.15 Storing lyfil puck in Collection Clamp to tube 1 @12:29:36.33 SC.reconfigure SCtight @12:29:36.39 SC.seek 1 @12:29:38.51 SC.reconfigure SCloose @12:29:38.72 Elbow.reconfigure TightElbow @12:29:38.78 Elbow.seek 1 @12:29:40.71 Forearm.reconfigure TightForearm @12:29:40.77 Forearm.seek 1 + 700 counts @12:29:41.82 SE.reconfigure SEpick @12:29:41.91 SE.seek beamBreak @12:29:45.06 SE.configure SEconfig @12:29:45.22 Elbow.reconfigure LimpElbow @12:29:45.28 Forearm.reconfigure LooseForearm @12:29:45.34 Forearm.seek 1 @12:29:45.64 Elbow.hold @12:29:45.71 Elbow.reconfigure TightElbow @12:29:45.82 SE.seek 0.03 Pucks below Top Plate @12:29:46.26 Forearm.force -20 @12:29:46.31 Forearm.seek 1 + 349 counts @12:29:46.64 Forearm.reconfigure TightForearm @12:29:46.70 Forearm.seek 1 + 700 counts @12:29:46.96 SE.seek 1.03 Pucks below Top Plate @12:29:47.55 Forearm.reconfigure LooseForearm @12:29:47.61 Forearm.seek :retract @12:29:48.52 Elbow.seek CC @12:29:50.23 Elbow.reconfigure LooseElbow @12:29:50.34 Forearm.seek CC @12:29:51.34 Hand.close @12:29:52.15 CC.seek :open @12:29:56.65 Forearm.seek :retract @12:29:57.63 Elbow.reconfigure TightElbow @12:29:57.69 Elbow.seek 1 @12:29:59.70 Elbow.reconfigure LimpElbow @12:29:59.77 Forearm.seek 1 @12:30:00.79 Elbow.hold @12:30:00.87 Elbow.reconfigure TightElbow @12:30:00.92 Hand.open @12:30:01.76 Forearm.force -20 @12:30:01.81 Forearm.seek 1 + 346 counts @12:30:02.19 Forearm.reconfigure TightForearm @12:30:02.25 Forearm.seek 1 + 700 counts @12:30:02.60 SE.seek :down @12:30:03.74
Forearm.reconfigure LooseForearm @12:30:03.80 Forearm.seek :retract @12:30:05.30 SE.coast @12:30:05.79
SC.coast @12:30:05.86 PSV.dial :air @12:30:07.20 PS.seek 0.499ml @12:30:13.23 delay 3 seconds @12:30:16.25 PSV.dial :mixloop @12:30:16.77 PRV.dial :air @12:30:17.31 delay 3 seconds @12:30:20.33 PRV.dial CBV @12:30:20.85 CBV.dial AIV @12:30:21.39 PRV.dial between CBV and air @12:30:21.76 PRV.dial :air @12:30:22.16 PS.reconfigure PSslow @12:30:22.22 PS.seek 0.599ml @12:30:24.76 PRV.dial :waste2 @12:30:25.59 PS.reconfigure PSconfig @12:30:25.65 PS.seek :empty @12:30:32.73 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:30:32.81 Hand.open @12:30:33.13 Elbow.seek FlushPuck::Garage @12:30:35.14 Elbow.reconfigure LooseElbow @12:30:35.25 Forearm.seek :garage @12:30:36.27 Hand.close @12:30:37.13 Forearm.seek :retract @12:30:38.17 Elbow.reconfigure TightElbow @12:30:38.23 Elbow.seek CC @12:30:41.14 Elbow.reconfigure LooseElbow @12:30:41.25 Forearm.reconfigure TightForearm @12:30:41.31 Forearm.seek CC @12:30:42.54 CC.seek :closed @12:30:46.80 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.32 @12:30:46.88 Hand.open @12:30:47.71 Forearm.reconfigure LooseForearm @12:30:47.77 Forearm.seek :retract @12:30:48.79 Clearing PML for modified lysate @12:30:48.80 CTV.dial :air,avoiding: :intake @12:30:48.82 PRV.dial :waste2 @12:30:48.85 PTV.dial :PRV,avoiding: :puck @12:30:49.71 delay 2 seconds @12:30:51.73 PTV.dial :PRVmixing,avoiding: :puck @12:30:52.57 delay 2 seconds @12:30:54.59 PSV.dial :PML @12:30:54.60 delay 2 seconds @12:30:56.63 PRV.dial :air @12:30:57.46 delay 2 seconds @12:30:59.53 PS.seek 1.499ml @12:31:15.98 delay 5 seconds @12:31:21.01 PS.seek 2.5ml @12:31:32.17 PSV.dial :air @12:31:32.67 PS.seek 0ml @12:31:59.51 mixing lysate with speDil @12:31:59.52 PSV.dial :PML @12:32:00.06 CBV.dial :PRV @12:32:00.07 PRV.dial :air @12:32:00.10 delay 2 seconds @12:32:02.13 PRV.dial :speDil @12:32:02.65 delay 2 seconds @12:32:05.15 PS.reconfigure PSslow1 @12:32:05.23 PS.seek 0.225ml @12:32:29.17 delay 5 seconds @12:32:34.19 PRV.dial CBV @12:32:35.06 delay 2 seconds @12:32:37.14 PS.seek 0.475ml @12:33:03.07 delay 5 seconds @12:33:08.09 PRV.dial :air @12:33:08.62 delay 2 seconds @12:33:10.65 PS.seek 0.965ml @12:34:00.70 delay 10 seconds @12:34:10.72 PRV.dial CBV @12:34:11.23 CTV.dial :air,avoiding: :intake @12:34:11.24 PSV.dial :air @12:34:11.78 PS.reconfigure PSconfig @12:34:11.84 PS.seek 2.5ml @12:34:28.67 delay 3 seconds @12:34:31.69 PSV.dial PTV @12:34:32.53 PTV.dial :PRV,avoiding: :puck @12:34:33.38 PS.seek 0ml @12:35:00.20 PSV.dial :air @12:35:01.05 PS.seek 2.5ml @12:35:27.98 delay 3 seconds @12:35:31.00 PSV.dial PTV @12:35:31.84 PTV.dial :PRV,avoiding: :puck @12:35:31.86 PS.seek 0ml @12:35:58.73 Pushing modified lysate into AIV Injection Loop @12:35:58.74 CBV.dial AIV @12:35:58.77 AIV.dial :bypass @12:35:58.79 PRV.dial CBV @12:35:58.81 PSV.dial :air @12:35:59.68 PS.seek 2.5ml @12:36:26.42 delay 3 seconds @12:36:29.44 PSV.dial :PML @12:36:29.95 PTV.dial :PRVmixing,avoiding: :puck @12:36:30.85 PS.reconfigure PSslow1 @12:36:30.91 PS.seek 1.74ml @12:37:48.29 delay 5 seconds @12:37:53.31 AIV.dial :sample @12:37:53.96 AWV.select :waste @12:37:54.13 PS.reconfigure PSslow2 @12:37:54.19 PS.seek 1.22ml @12:39:41.14 delay 15 seconds @12:39:56.16 AIV.dial :bypass @12:39:56.80 PS.reconfigure PSconfig @12:39:56.86 PS.seek 0ml @12:40:10.48 PSV.dial :air @12:40:11.00 PS.seek 2.5ml @12:40:37.70 PSV.dial :PML @12:40:38.23 PS.seek 0ml @12:41:05.26 AIV.dial :coilToMFB @12:41:05.27 AWV.select :air @12:41:05.45 Extracting Nucleic Acid from AIV @12:41:05.48 SPE.hold 2:46:40,55C @12:41:05.51
Beginning samplePassD @12:41:05.58 ASV.dial :SPEdelivery @12:41:05.69
AWV.select :air @12:41:05.71 AIV.dial :bypass @12:41:05.73 PSV.dial PSR @12:41:06.04 delay 20 seconds @12:41:07.05
Pulling 1.5ml of PSR.killMFB @12:41:07.06 PSR.select :killMFB @12:41:07.21 PS.seek 1.5ml @12:41:24.30 delay 5 seconds @12:41:26.06 primeLysateINT @12:41:26.07 AWV.select :air @12:41:26.09 AIV.dial :coilToMFB @12:41:26.12 ATV.dial AIV @12:41:28.04 AS.reconfigure ASslow1 @12:41:28.10 AS.seek 0.299ml @12:41:29.32
PSV.dial :waste1 @12:41:29.88 PS.seek 0ml @12:41:46.95 PSV.dial PSR @12:41:47.50 PSR.select :air @12:41:47.63 PS.seek 2.5ml @12:42:15.67 PRV.dial CBV @12:42:15.69
CBV.dial PRV @12:42:15.72 PSV.dial :mixloop @12:42:17.41 CTV.dial :air,avoiding: :intake @12:42:17.42 PTV.dial :PRVmixing @12:42:17.46 PS.seek 0ml @12:42:28.73 delay 10 seconds @12:42:38.76 ATV.dial :air2 @12:42:39.66 AS.seek 0.329ml @12:42:45.83
PSV.dial PSR @12:42:45.99 ATV.dial :waste2 @12:42:46.70 AS.reconfigure ASnorm @12:42:46.76 AS.seek :empty @12:42:47.13
Pulling 1.5ml of PSR.flush @12:42:47.14 PSR.select :flush @12:42:47.30 PS.seek 1.5ml @12:43:03.77 Lysate primed! @12:43:03.78 AFV.select :holdingCoil @12:43:03.80 ATV.dial :air2 @12:43:04.48
delay 5 seconds @12:43:04.49 AS.reconfigure ASslow1 @12:43:04.57 AS.seek 0.069ml @12:43:09.50
PSV.dial :waste1 @12:43:10.20 PS.seek 0ml @12:43:18.87 delay 3 seconds @12:43:21.89 ATV.dial :AIV @12:43:22.79 AS.seek 0.469ml @12:43:27.30
PSV.dial PSR @12:43:27.84 PSR.select :air @12:43:27.97 PS.seek 2.5ml @12:43:56.02 PRV.dial CBV @12:43:56.05
CBV.dial PRV @12:43:56.07 PSV.dial :mixloop @12:43:57.31 CTV.dial :air,avoiding: :intake @12:43:57.32 PTV.dial :PRVmixing @12:43:57.36 PS.seek 0ml @12:44:25.36 PSV.dial PSR @12:44:26.63 Pulling 1.5ml of PSR.flush @12:44:26.64 PSR.select :flush @12:44:26.79 PS.seek 1.5ml @12:44:43.54 delay 5 seconds @12:44:43.80
delay 5 seconds @12:44:48.60 Solid Phase Extraction Heater at 47.1C @12:44:48.61 ATV.dial ASV @12:44:48.82
PSV.dial :waste1 @12:44:49.04 ASV.dial :SPEdelivery @12:44:49.13 AS.seek 0.041ml @12:44:49.37
PS.seek 0ml @12:45:06.33 PSV.dial PSR @12:45:06.99 PSR.select :air @12:45:07.12 PS.seek 2.5ml @12:45:34.61 PRV.dial CBV @12:45:34.63
CBV.dial PRV @12:45:34.66 PSV.dial :mixloop @12:45:35.89 CTV.dial :air,avoiding: :intake @12:45:35.90 PTV.dial :PRVmixing @12:45:35.94 PS.seek 0ml @12:46:03.46 PSV.dial PSR @12:46:04.72 Pulling 1.5ml of PSR.flush @12:46:04.73 PSR.select :flush @12:46:04.89 PS.seek 1.5ml @12:46:15.49 delay 10 seconds @12:46:21.64
delay 5 seconds @12:46:25.51 rinseLysateLineINT @12:46:25.52 AFV.select :carrierSolution @12:46:25.64 delay 2 seconds @12:46:26.66
PSV.dial :waste1 @12:46:27.20 PS.seek 0ml @12:46:27.66 AS.reconfigure ASconfig @12:46:27.72 AS.seek 1ml @12:46:43.83
PSV.dial PSR @12:46:44.36 PSR.select :air @12:46:44.49 PS.seek 2.5ml @12:47:11.67 PRV.dial CBV @12:47:11.69
CBV.dial PRV @12:47:11.73 PSV.dial :mixloop @12:47:12.92 CTV.dial :air,avoiding: :intake @12:47:12.93 PTV.dial :PRVmixing @12:47:12.96 PS.reconfigure PSslow @12:47:13.04 PS.seek 1.2ml @12:47:23.15 delay 1 second @12:47:24.17 AFV.select :holdingCoil @12:47:24.66 delay 1 second @12:47:25.74 ATV.dial AIV @12:47:26.42 AIV.dial :coilToMFB @12:47:26.43 AWV.select :waste @12:47:26.56 AS.reconfigure ASslow0 @12:47:26.62 AS.seek 0ml @12:47:39.83
PSV.dial :waste1 @12:47:41.33 PS.reconfigure PSconfig @12:47:41.40 PS.seek 0ml @12:47:54.64 PSV.dial :air @12:47:55.95 PS.seek 2.5ml @12:48:22.92 PSV.dial :mixloop @12:48:23.46 PS.reconfigure PSslow @12:48:23.52 PS.seek 0ml @12:49:07.51 ATV.dial :air2 @12:49:08.21 AS.reconfigure ASslow1 @12:49:08.27 AS.seek 0.05ml @12:49:14.71
PSV.dial :air @12:49:15.22 PS.reconfigure PSconfig @12:49:15.28 PS.seek 2.5ml @12:49:18.62 ATV.dial :AIV @12:49:19.57 AS.seek 0.01ml @12:49:27.92 ATV.dial :waste @12:49:29.59 AS.seek :empty @12:49:32.04 ethanol wash @12:49:32.05 ATV.dial :air2 @12:49:33.36 AS.reconfigure ASslow2 @12:49:33.42 AS.seek 0.009ml @12:49:42.18
PSV.dial :mixloop @12:49:42.69 PS.reconfigure PSslow @12:49:42.75 PS.seek 0ml @12:49:43.81 delay 3 seconds @12:49:46.83 Pulling 0.4ml of Ethanol Wash @12:49:46.84 ATV.dial :ethanol @12:49:47.68 AS.reconfigure ASslow1 @12:49:47.74 AS.seek 0.409ml @12:50:33.93
Cleaning Collection Syringe @12:50:33.94 CSV.dial CSR @12:50:34.52 Pulling 4ml of CSR.flush @12:50:34.53 CSR.select :flush @12:50:34.68 CS.seek 4ml @12:50:46.05 delay 5 seconds @12:50:51.07 CSR.select :air @12:50:51.21 CS.seek 10ml @12:51:07.61 CSV.dial CTV @12:51:07.64
CTV.dial CSV @12:51:08.45 delay 3 seconds @12:51:09.23
CBV.dial :waste1 @12:51:09.77 CS.seek 0ml @12:51:11.47 ATV.dial :air1 @12:51:11.87 delay 3 seconds @12:51:14.94 AS.seek 0.434ml @12:51:20.32 delay 3 seconds @12:51:23.34 ATV.dial :ethanol @12:51:23.96 AS.seek 0.419ml @12:51:27.40 delay 3 seconds @12:51:30.42 ATV.dial ASV @12:51:31.79 AS.seek 0.092ml @12:51:36.30
CSV.dial CSR @12:51:36.89 Pulling 4ml of CSR.flush @12:51:36.90 CSR.select :flush @12:51:37.05 CS.seek 4ml @12:51:48.39 delay 5 seconds @12:51:53.41 CSR.select :air @12:51:53.55 CS.seek 10ml @12:52:09.95 CSV.dial CTV @12:52:09.98
CTV.dial CSV @12:52:10.52 CBV.dial :waste1 @12:52:10.54 CS.seek 0ml @12:52:37.35 Garaging FlushPuck in Collection Clamp @12:52:37.44 Elbow.reconfigure TightElbow @12:52:37.50 Elbow.seek CC @12:52:37.75 Elbow.reconfigure LooseElbow @12:52:37.88 delay 3 seconds @12:52:37.90
Forearm.seek CC @12:52:38.92 Hand.close @12:52:39.67 CC.seek :open @12:52:40.91 ATV.dial :waste2 @12:52:41.68 AS.reconfigure ASconfig @12:52:41.74 AS.seek :empty @12:52:44.06
Forearm.seek :retract @12:52:45.06 Elbow.reconfigure TightElbow @12:52:45.12 Elbow.seek :garage @12:52:47.42 ATV.dial :air2 @12:52:47.82 AS.reconfigure ASslow1 @12:52:47.89 AS.seek 1ml @12:52:48.26
Elbow.reconfigure LooseElbow @12:52:48.36 Forearm.reconfigure TightForearm @12:52:48.42 Forearm.seek :garage @12:52:49.59 Hand.open @12:52:50.36 Forearm.reconfigure LooseForearm @12:52:50.42 Forearm.seek :retract @12:52:51.46 Skipping Whole Cell Archive @12:52:51.47 Starting finalPurge @12:52:51.49 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:52:51.59 Hand.open @12:52:51.86 Elbow.reconfigure TightElbow @12:52:51.92 Elbow.seek FlushPuck::Garage @12:52:52.06 Elbow.reconfigure LooseElbow @12:52:52.17 Forearm.seek :garage @12:52:53.20 Hand.close @12:52:54.03 Forearm.seek :retract @12:52:55.09 Elbow.reconfigure TightElbow @12:52:55.15 Elbow.seek CC @12:52:58.08 Elbow.reconfigure LooseElbow @12:52:58.20 Forearm.reconfigure TightForearm @12:52:58.26 Forearm.seek CC @12:52:59.52 CC.seek :closed @12:53:03.79 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.32 @12:53:03.87 Hand.open @12:53:04.73 Forearm.reconfigure LooseForearm @12:53:04.79 Forearm.seek :retract @12:53:05.81 Flushing the Collection Valve::Manifold @12:53:05.94 CSV.dial CSR @12:53:06.50 Pulling 5ml of CSR.flush @12:53:06.51 CSR.select :flush @12:53:06.66 CS.seek 5ml @12:53:20.55 delay 5 seconds @12:53:25.57 CSR.select :air @12:53:25.75 CS.reconfigure CSfast @12:53:25.81 CS.seek 7.501ml @12:53:33.17 CSV.dial :waste @12:53:34.69 CS.reconfigure CSconfig @12:53:34.76 CS.seek 0ml @12:53:54.95 Flushing lower manifold's waste1 port @12:53:55.09 CSV.dial :flush @12:53:55.61 CS.reconfigure CSfast @12:53:55.67 CS.seek 5ml @12:54:09.86 delay 5 seconds @12:54:14.88 CSV.dial :air @12:54:15.47 CS.seek 7ml @12:54:21.64 CSV.dial CTV @12:54:21.67
CTV.dial CSV @12:54:22.17 CBV.dial :waste1 @12:54:22.19 CS.seek 0ml @12:54:41.05 Flushing lower manifold's waste2b port @12:54:41.19 CSV.dial :flush @12:54:42.06 CS.seek 5ml @12:54:55.86 delay 5 seconds @12:55:00.88 CSV.dial :air @12:55:01.48 CS.seek 7ml @12:55:07.64 CSV.dial CTV @12:55:07.67
CTV.dial CSV @12:55:08.17 CBV.dial :waste2b @12:55:09.04 CS.seek 0ml @12:55:27.86 Flushing lower manifold's waste1 port @12:55:27.87 CSV.dial CSR @12:55:28.46 Pulling 4ml of CSR.mfbkill @12:55:28.47 CSR.select :mfbkill @12:55:28.62 CS.reconfigure CSconfig @12:55:28.68 CS.seek 4ml @12:55:39.99 delay 5 seconds @12:55:45.01 CSR.select :air @12:55:45.14 CS.reconfigure CSfast @12:55:45.20 CS.seek 10ml @12:56:01.49 CSV.dial CTV @12:56:01.52
CTV.dial CSV @12:56:02.06 CBV.dial :waste1 @12:56:02.92 CS.seek 0ml @12:56:09.46 delay 5 seconds @12:56:14.48 ATV.dial ASV @12:56:15.50 ASV.dial :SPEdelivery @12:56:15.52 AS.seek 0.02ml @12:56:29.43
Flushing lower manifold's waste2b port @12:56:29.44 CSV.dial CSR @12:56:30.15 Pulling 4ml of CSR.mfbkill @12:56:30.16 CSR.select :mfbkill @12:56:30.31 CS.reconfigure CSconfig @12:56:30.37 CS.seek 4ml @12:56:41.55 delay 5 seconds @12:56:46.57 CSR.select :air @12:56:46.71 CS.reconfigure CSfast @12:56:46.77 CS.seek 10ml @12:57:03.05 CSV.dial CTV @12:57:03.08
CTV.dial CSV @12:57:03.60 CBV.dial :waste2b @12:57:04.48 CS.seek 0ml @12:57:31.08 Killing upper manifold @12:57:31.09 CSV.dial CSR @12:57:31.68 Pulling 4ml of CSR.mfbkill @12:57:31.69 CSR.select :mfbkill @12:57:31.84 CS.reconfigure CSconfig @12:57:31.90 CS.seek 4ml @12:57:43.11 delay 5 seconds @12:57:48.13 CSR.select :air @12:57:48.27 CS.reconfigure CSfast @12:57:48.33 CS.seek 10ml @12:58:04.68 CSV.dial CBV @12:58:04.70
CBV.dial CSV @12:58:05.93 CTV.dial :air @12:58:07.48 CS.seek 0ml @12:58:33.99 delay 7 seconds @12:58:41.02 CS.seek 3ml @12:58:49.74 CSV.dial :waste @12:58:50.26 CS.seek 0ml @12:58:58.93 Flushing upper manifold @12:58:59.06 CSV.dial :flush @12:58:59.58 CS.seek 5ml @12:59:13.30 delay 5 seconds @12:59:18.32 CSV.dial :air @12:59:18.91 CS.seek 7ml @12:59:24.96 CSV.dial CBV @12:59:24.99
CBV.dial CSV @12:59:26.21 CTV.dial :air @12:59:26.70 CS.seek 0ml @12:59:32.89 delay 5 seconds @12:59:37.91 ATV.dial :air2 @12:59:38.98 AS.seek 0.52ml @12:59:45.43
delay 7 seconds @12:59:52.47 CS.seek 3ml @13:00:01.08 CSV.dial :waste @13:00:01.63 CS.seek 0ml @13:00:10.54 CSV.dial :flush @13:00:11.06 CS.seek 3ml @13:00:19.77 delay 5 seconds @13:00:24.79 CSV.dial :air @13:00:25.35 CS.seek 5ml @13:00:31.37 CSV.dial :air @13:00:31.39 CS.seek 0ml @13:00:45.08 delay 7 seconds @13:00:52.11 CS.seek 3ml @13:01:00.74 CSV.dial :waste @13:01:01.61 CS.seek 0ml @13:01:10.18 Flushing lower manifold's waste1 port @13:01:10.32 CSV.dial :flush @13:01:10.86 CS.seek 5ml @13:01:19.75 delay 5 seconds @13:01:24.55
delay 5 seconds @13:01:24.77 ATV.dial ASV @13:01:25.78 ASV.dial :wasteR @13:01:26.18 AS.seek 0.3ml @13:01:29.57
CSV.dial :air @13:01:30.14 CS.seek 7ml @13:01:36.27 CSV.dial CTV @13:01:36.30
CTV.dial CSV @13:01:37.91 CBV.dial :waste1 @13:01:38.44 CS.seek 0ml @13:01:57.09 Flushing lower manifold's waste2b port @13:01:57.23 CSV.dial :flush @13:01:58.10 CS.seek 5ml @13:02:10.67 delay 20 seconds @13:02:11.93
delay 5 seconds @13:02:16.95 CSV.dial :air @13:02:17.54 CS.seek 7ml @13:02:23.62 CSV.dial CTV @13:02:23.65
CTV.dial CSV @13:02:24.16 CBV.dial :waste2b @13:02:25.03 CS.seek 0ml @13:02:30.70 AS.seek 0.07ml @13:02:43.71
Garaging FlushPuck in Collection Clamp @13:02:43.80 Elbow.reconfigure TightElbow @13:02:43.86 Elbow.seek CC @13:02:44.14 Elbow.reconfigure LooseElbow @13:02:44.26 Forearm.seek CC @13:02:45.26 Hand.close @13:02:46.06 CC.seek :open @13:02:50.45 Forearm.seek :retract @13:02:51.46 Elbow.reconfigure TightElbow @13:02:51.52 Elbow.seek :garage @13:02:54.62 Elbow.reconfigure LooseElbow @13:02:54.73 Forearm.reconfigure TightForearm @13:02:54.79 Forearm.seek :garage @13:02:55.95 Hand.open @13:02:56.74 Forearm.reconfigure LooseForearm @13:02:56.80 Forearm.seek :retract @13:02:57.88 Starting initialPurge @13:02:57.89 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:02:57.98 Hand.open @13:02:58.74 Elbow.reconfigure TightElbow @13:02:58.80 Elbow.seek FlushPuck::Garage @13:02:58.99 Elbow.reconfigure LooseElbow @13:02:59.09 Forearm.seek :garage @13:03:00.13 Hand.close @13:03:01.06 Forearm.seek :retract @13:03:02.06 Elbow.reconfigure TightElbow @13:03:02.12 Elbow.seek CC @13:03:05.11 Elbow.reconfigure LooseElbow @13:03:05.23 Forearm.reconfigure TightForearm @13:03:05.29 Forearm.seek CC @13:03:06.57 CC.seek :closed @13:03:10.83 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.32 @13:03:10.91 Hand.open @13:03:11.74 Forearm.reconfigure LooseForearm @13:03:11.80 Forearm.seek :retract @13:03:12.79 Flushing the Collection Valve::Manifold @13:03:12.93 CSV.dial CSR @13:03:13.50 Pulling 5ml of CSR.flush @13:03:13.51 CSR.select :flush @13:03:13.66 CS.reconfigure CSconfig @13:03:13.72 CS.seek 5ml @13:03:17.21 delay 20 seconds @13:03:27.52
delay 5 seconds @13:03:32.54 CSR.select :air @13:03:32.71 CS.reconfigure CSfast @13:03:32.77 CS.seek 7.5ml @13:03:37.23 ATV.dial :waste2 @13:03:38.01 AS.reconfigure ASconfig @13:03:38.07 AS.seek :empty @13:03:40.09
CSV.dial :waste @13:03:41.59 CS.reconfigure CSconfig @13:03:41.65 CS.seek 0ml @13:03:42.47 Elution @13:03:42.48 ATV.dial :air1 @13:03:43.62 AS.reconfigure ASslow1 @13:03:43.69 AS.seek 0.049ml @13:03:54.11 delay 3 seconds @13:03:57.13 ATV.dial :ElutionWater @13:03:57.82 AS.seek 0.109ml @13:04:01.84
Purging external Intake @13:04:01.97 CSV.dial :flush @13:04:02.48 CS.reconfigure CSfast @13:04:02.54 CS.seek 10ml @13:04:10.15 delay 3 seconds @13:04:13.17 ATV.dial ASV,via: :air2 @13:04:15.32 ASV.dial :SPEelution @13:04:15.92 AFV.select :carrierSolution @13:04:16.08 AS.reconfigure ASnorm @13:04:16.14 AS.seek 0.759ml @13:04:28.93
delay 5 seconds @13:04:33.95 CSV.dial CBV @13:04:33.99
CBV.dial CSV @13:04:34.90 CTV.dial :intake @13:04:35.76 Intake.select :open @13:04:35.89 CS.seek 0ml @13:04:49.20 delay 3 seconds @13:04:52.22 AFV.select :holdingCoil @13:04:52.37 AS.reconfigure ASslow1 @13:04:52.43 AS.seek 0.474ml @13:05:03.12
Intake.select :close @13:05:03.25 CTV.dial :air @13:05:04.10 Flushing upper manifold @13:05:04.23 CSV.dial :flush @13:05:05.11 CS.seek 5ml @13:05:18.99 delay 5 seconds @13:05:24.01 CSV.dial :air @13:05:25.19 CS.seek 7ml @13:05:31.31 CSV.dial CBV @13:05:31.33
CBV.dial CSV @13:05:32.51 CTV.dial :air @13:05:32.53 CS.seek 0ml @13:05:49.98 delay 5 seconds @13:05:51.62
delay 7 seconds @13:05:55.06 AS.reconfigure ASslow2 @13:05:55.12 AS.seek 0.439ml @13:05:58.65
CS.seek 3ml @13:06:07.21 CSV.dial :waste @13:06:07.74 CS.seek 0ml @13:06:16.41 CSV.dial :flush @13:06:16.94 CS.seek 3ml @13:06:25.75 delay 5 seconds @13:06:30.76 delay 1:00 @13:06:30.79
CSV.dial :air @13:06:31.35 CS.seek 5ml @13:06:37.40 CSV.dial :air @13:06:37.42 CS.seek 0ml @13:06:51.03 delay 7 seconds @13:06:58.06 CS.seek 3ml @13:07:06.53 CSV.dial :waste @13:07:07.40 CS.seek 0ml @13:07:15.90 Garaging FlushPuck in Collection Clamp @13:07:15.99 Elbow.reconfigure TightElbow @13:07:16.05 Elbow.seek CC @13:07:16.31 Elbow.reconfigure LooseElbow @13:07:16.42 Forearm.seek CC @13:07:17.42 Hand.close @13:07:18.19 CC.seek :open @13:07:22.54 Forearm.seek :retract @13:07:23.54 Elbow.reconfigure TightElbow @13:07:23.60 Elbow.seek :garage @13:07:26.77 Elbow.reconfigure LooseElbow @13:07:26.88 Forearm.reconfigure TightForearm @13:07:26.94 Forearm.seek :garage @13:07:28.11 Hand.open @13:07:28.87 Forearm.reconfigure LooseForearm @13:07:28.93 Forearm.seek :retract @13:07:29.98 Completed [coreNEG] begun 11:54:46 @13:07:29.99 Extending 18tun until completion of MFBPCR @13:07:30.78 pushing eluate to holding coil @13:07:30.86 AS.seek 0.279ml @13:10:11.80 delay 30 seconds @13:10:41.83 ATV.dial :waste,via: :air3 @13:10:43.58 AS.reconfigure ASconfig @13:10:43.64 AS.seek :empty @13:11:00.05 Parking Bleach in LysateSource line and SPEcolumn @13:11:00.06 ATV.dial :air3 @13:11:00.91 AS.reconfigure ASslow1 @13:11:00.98 AS.seek 0.029ml @13:11:07.33 ATV.dial :ElutionWater @13:11:07.93 AS.seek 0.059ml @13:11:14.22 ATV.dial :air3 @13:11:14.84 AS.seek 0.069ml @13:11:17.12 delay 2 seconds @13:11:19.14 ATV.dial :bleach @13:11:19.60 AS.seek 0.769ml @13:12:50.41 Session Ended @13:13:40.64 delay 3 seconds @13:13:43.66 ATV.dial :air3 @13:13:44.20 delay 3 seconds @13:13:47.27 AS.seek 0.794ml @13:13:52.56 delay 3 seconds @13:13:55.58 ATV.dial :bleach @13:13:56.18 AS.seek 0.779ml @13:13:59.44 delay 3 seconds @13:14:02.46 ATV.dial :AIV @13:14:03.72 AS.reconfigure ASconfig @13:14:03.77 AS.seek 0.015ml @13:14:47.98 ATV.dial :waste @13:14:49.48 AS.seek :empty @13:14:50.69 ATV.dial :air3 @13:14:51.35 AS.reconfigure ASslow1 @13:14:51.86 AS.seek 0.009ml @13:14:54.16 delay 2 seconds @13:14:56.18 ATV.dial :bleach @13:14:56.64 AS.seek 0.579ml @13:16:51.50 delay 3 seconds @13:16:54.52 ATV.dial :air3 @13:16:55.06 delay 3 seconds @13:16:58.13 AS.seek 0.604ml @13:17:03.42 delay 3 seconds @13:17:06.44 ATV.dial :bleach @13:17:07.04 AS.seek 0.589ml @13:17:10.34 delay 3 seconds @13:17:13.36 ATV.dial :ASV @13:17:14.41 ASV.dial :wasteR @13:17:15.01 AS.seek 0ml @13:19:13.75 delay 1:00 @13:20:13.78 AFV.select :carrierSolution @13:20:13.88 delay 2 seconds @13:20:15.90 AS.reconfigure ASconfig @13:20:15.95 AS.seek 1ml @13:21:13.70 delay 1 second @13:21:14.72 AFV.select :holdingCoil @13:21:14.82 delay 1 second @13:21:15.84 ATV.dial ASV @13:21:15.85 ASV.dial :wasteR @13:21:15.87 AS.reconfigure ASslow1 @13:21:15.91 AS.seek 0ml @13:24:37.28 delay 20 seconds @13:24:57.31 ATV.dial :air3 @13:24:58.83 AS.seek 0.59ml @13:25:11.93 Session Begun @13:25:17.26 -> showlog 100 @13:26:57.77 delay 3 seconds @13:27:00.79 ATV.dial :ASV @13:27:02.24 ASV.dial :wasteR @13:27:02.33 AS.seek 0.09ml @13:28:43.16 delay 30 seconds @13:29:13.19 ATV.dial :extSrc @13:29:14.08 delay 3 seconds @13:29:17.10 ATV.dial :ElutionWater @13:29:18.24 AS.seek 0.12ml @13:29:24.57 ATV.dial :air3 @13:29:25.19 AS.seek 0.13ml @13:29:27.49 delay 2 seconds @13:29:29.51 ATV.dial :bleach @13:29:30.00 AS.seek 0.23ml @13:29:50.39 delay 3 seconds @13:29:53.41 ATV.dial :air3 @13:29:53.97 delay 3 seconds @13:29:57.04 AS.seek 0.255ml @13:30:02.34 delay 3 seconds @13:30:05.36 ATV.dial :bleach @13:30:05.99 AS.seek 0.24ml @13:30:09.24 delay 3 seconds @13:30:12.26 ATV.dial :ASV @13:30:13.32 ASV.dial :SPEdelivery @13:30:13.71 AFV.select :carrierSolution @13:30:13.82 delay 2 seconds @13:30:15.85 AS.reconfigure ASconfig @13:30:15.90 AS.seek 0.5ml @13:30:31.19 delay 1 second @13:30:32.21 AFV.select :holdingCoil @13:30:32.36 AS.reconfigure ASslow1 @13:30:32.42 AS.seek 0.17ml @13:31:39.08 SPE.hold 2:46:40,95C @13:31:39.15 delay 3:00 @13:34:39.18 AFV.select :carrierSolution @13:34:39.30 delay 2 seconds @13:34:41.32 AS.reconfigure ASconfig @13:34:41.38 AS.seek 1ml @13:35:29.38 delay 1 second @13:35:30.40 AFV.select :holdingCoil @13:35:30.51 delay 1 second @13:35:31.53 ASV.dial :wasteD @13:35:32.63 AS.seek 0ml @13:36:30.43 Priming Eluate @13:36:30.44 AFV.select :carrierSolution @13:36:30.56 delay 2 seconds @13:36:32.59 AS.seek 0.5ml @13:37:01.63 delay 1 second @13:37:02.65 AFV.select :holdingCoil @13:37:02.75 ATV.dial ASV @13:37:02.76 ASV.dial :SPErecovery @13:37:03.40 Swish #1 of 2 @13:37:03.48 AS.reconfigure ASslow0 @13:37:03.54 AS.seek 0.6ml @13:37:13.92 AS.seek 0.5ml @13:37:24.28 Swish #2 of 2 @13:37:24.37 AS.seek 0.6ml @13:37:34.85 AS.seek 0.5ml @13:37:45.22 delay 3 seconds @13:37:48.29 AS.reconfigure ASslow1 @13:37:48.35 AS.seek 0.684ml @13:38:25.68 delay 3 seconds @13:38:28.70 Eluate primed @13:38:28.71 ATV.dial :waste2 @13:38:29.77 AS.reconfigure ASnorm @13:38:29.89 AS.seek 0ml @13:39:04.70 SPE.coast @13:39:04.79 ASV.dial :SPErecovery @13:39:04.81 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118aug2613h.pcr @13:39:04.83 Building PCR Slug from ASV with MM2 mix, PP4 primer @13:39:04.85 Priming with carrier @13:39:04.88 ATV.dial ARV @13:39:05.86 ARV.dial :carrier @13:39:06.23 AS.seek 0.5ml @13:39:31.80 AFV.select :carrierSolution @13:39:31.92 delay 2 seconds @13:39:33.95 AS.reconfigure ASconfig @13:39:34.01 AS.seek 1ml @13:40:03.07 delay 1 second @13:40:04.09 AFV.select :holdingCoil @13:40:04.19 delay 1 second @13:40:05.21 ATV.dial :waste @13:40:06.71 AS.reconfigure ASnorm @13:40:06.77 AS.seek :empty @13:40:57.52 AFV.select :carrierSolution @13:40:57.63 delay 2 seconds @13:40:59.66 AS.reconfigure ASconfig @13:40:59.72 AS.seek 0.5ml @13:41:28.85 delay 1 second @13:41:29.87 AFV.select :holdingCoil @13:41:29.97 Building Slug @13:41:29.99 ATV.dial :ARV @13:41:31.74 delay 2 seconds @13:41:33.76 ARV.dial :air1 @13:41:34.21 delay 2 seconds @13:41:36.28 AS.reconfigure ASslow2 @13:41:36.35 AS.seek 0.506ml @13:41:42.58 delay 2 seconds @13:41:44.60 ARV.dial :MM2 @13:41:46.13 delay 2 seconds @13:41:48.15 Adding MM2 @13:41:48.25 AS.seek 0.524ml @13:42:06.62 delay 25 seconds @13:42:31.64 ARV.dial :PP4 @13:42:32.04 Adding PP4 @13:42:32.05 delay 2 seconds @13:42:34.13 AS.seek 0.53ml @13:42:40.58 delay 10 seconds @13:42:50.61 ARV.dial :air3 @13:42:51.01 adding ARV air @13:42:51.02 delay 2 seconds @13:42:53.10 AS.seek 0.536ml @13:42:59.37 delay 5 seconds @13:43:04.39 ARV.dial :carrier @13:43:05.69 delay 7 seconds @13:43:12.77 AS.seek 0.594ml @13:44:11.46 delay 5 seconds @13:44:16.48 ATV.dial :ASV @13:44:16.90 delay 2 seconds @13:44:18.97 AS.seek 0.6ml @13:44:25.22 delay 4 seconds @13:44:29.24 ATV.dial ARV @13:44:29.79 delay 2 seconds @13:44:31.81 ARV.dial :carrier @13:44:31.82 delay 2 seconds @13:44:33.90 AS.seek 0.65ml @13:45:24.33 delay 4 seconds @13:45:28.35 ATV.dial PCR @13:45:28.98 ASV.dial :waste1 @13:45:29.79 ARV.dial :air1 @13:45:30.19 Positioning Slug by 0.324ml @13:45:30.29 AS.seek 0.326ml @13:50:56.63 delay 5 seconds @13:51:01.65 ATV.dial ARV @13:51:02.43 ASV.dial :PCRlock @13:51:02.90 delay 5 seconds @13:51:07.92 Running Tt1 Profile60C300 thermocycling @13:51:07.94 PCR.program Profile60C300 @13:51:08.08 Profile60C300 should complete in < 1:45:21 @13:51:08.09 PCR.run 2:16:41.25 @15:07:27.49 Session Begun @15:07:31.67 -> showlog 100 @15:11:49.38 PCR.off @15:11:50.88 PCR for Tt1 completed -- closed /var/log/esp/Tt118aug2613h.pcr @15:11:50.97 Cleaning PCR @15:11:50.98 AFV.select :carrierSolution @15:11:51.12 delay 2 seconds @15:11:53.15 AS.reconfigure ASconfig @15:11:53.24 AS.seek 0.5ml @15:12:03.59 delay 1 second @15:12:04.61 AFV.select :holdingCoil @15:12:04.71 ASV.dial :waste1 @15:12:05.10 ATV.dial :air3 @15:12:06.41 AS.reconfigure ASslow1 @15:12:06.48 AS.seek 0.51ml @15:12:08.78 delay 2 seconds @15:12:10.80 ATV.dial :bleach @15:12:11.43 AS.seek 0.81ml @15:13:12.02 delay 3 seconds @15:13:15.04 ATV.dial :air3 @15:13:15.60 delay 3 seconds @15:13:18.67 AS.seek 0.835ml @15:13:24.02 delay 3 seconds @15:13:27.04 ATV.dial :bleach @15:13:28.16 AS.seek 0.82ml @15:13:31.49 delay 3 seconds @15:13:34.51 ARV.dial :waste @15:13:35.15 delay 3 seconds @15:13:38.17 ATV.dial :ARV @15:13:39.07 AS.reconfigure ASconfig @15:13:39.14 AS.seek 0.47ml @15:13:59.57 AFV.select :carrierSolution @15:13:59.70 delay 2 seconds @15:14:01.73 AS.seek 1ml @15:14:32.49 delay 1 second @15:14:33.51 AFV.select :holdingCoil @15:14:33.61 delay 1 second @15:14:34.64 AS.reconfigure ASfast @15:14:34.70 AS.seek 0ml @15:15:02.07 AFV.select :carrierSolution @15:15:02.20 delay 2 seconds @15:15:04.23 AS.reconfigure ASconfig @15:15:04.29 AS.seek 1ml @15:16:02.09 delay 1 second @15:16:03.11 AFV.select :holdingCoil @15:16:03.21 delay 1 second @15:16:04.24 AS.reconfigure ASfast @15:16:04.30 AS.seek 0ml @15:16:31.65 AFV.select :carrierSolution @15:16:31.78 delay 2 seconds @15:16:33.81 AS.reconfigure ASconfig @15:16:33.88 AS.seek 1ml @15:17:31.63 delay 1 second @15:17:32.65 AFV.select :holdingCoil @15:17:32.75 delay 1 second @15:17:33.78 AS.reconfigure ASfast @15:17:33.84 AS.seek 0ml @15:18:01.21 ARV.dial between carrier and air1 @15:18:01.73 ATV.dial :air3 @15:18:03.04 AS.reconfigure ASslow1 @15:18:03.11 AS.seek 0.01ml @15:18:05.48 delay 2 seconds @15:18:07.50 ATV.dial :bleach @15:18:08.12 AS.seek 0.81ml @15:20:44.19 -> AS.stop @15:20:44.20 AS.stop @15:20:44.36 Slide::Error in MFBPCR -- Analytical Syringe aborted at 0.786ml @15:20:44.39 Waiting to Resume from Checkpoint "Analytical Syringe aborted at 0.786ml" @15:20:44.41 BadNews.email "Analytical Syringe aborted at 0.786ml",Subject: "Slide::Error in MFBPCR" @15:21:02.49 -> Abort! "we want the lysate!" @15:21:02.51 NoMethodError in bkha5 -- undefined method `Abort!' for # @15:21:14.22 -> abort! "we want the lysate!" @15:21:14.23 CmdServer::Client::Error in bkha5 -- Aborting Mission: we want the lysate! @15:21:14.31 BadNews.email "we want the lysate!",Subject: "18tun ABORTED!" @15:21:21.11 Session Ended @15:21:21.15 Session Ended @19:41:31.24CEST26-Aug-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @19:41:32.94 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @19:41:33.19 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @19:41:38.21 Polling Can every 10:00 @19:41:39.96 SPE.coast @19:41:40.01 SPE.configure SPEconfig @19:41:40.09 AS.reconfigure ASconfig @19:41:40.13 AS.hold @19:41:40.17 AS.configure ASconfig @19:41:40.26 SS.reconfigure SSconfig @19:41:40.29 SS.hold @19:41:40.35 SS.configure SSconfig @19:41:40.43 CC.reconfigure CCconfig @19:41:40.47 CC.hold @19:41:40.55 CC.configure CCconfig @19:41:40.63 CS.reconfigure CSconfig @19:41:40.67 CS.hold @19:41:40.74 CS.configure CSconfig @19:41:41.16 PC.reconfigure PCconfig @19:41:41.21 PC.hold @19:41:41.31 PC.configure PCconfig @19:41:41.47 PS.configure PSconfig @19:41:41.67 Forearm.reconfigure LooseForearm @19:41:41.75 Forearm.hold @19:41:41.84 Forearm.configure LooseForearm @19:41:42.00 Elbow.reconfigure LooseElbow @19:41:42.06 Elbow.hold @19:41:42.11 Elbow.configure LooseElbow @19:41:42.21 SC.reconfigure SCloose @19:41:42.25 SC.hold @19:41:42.31 SC.configure SCloose @19:41:42.39 SE.reconfigure SEconfig @19:41:42.43 SE.hold @19:41:42.47 SE.configure SEconfig @19:41:42.51 CH.coast @19:41:42.56 CH.configure CHconfig @19:41:42.59 PH.coast @19:41:42.64 PH.configure PHconfig @19:41:42.67 PCR.off @19:41:42.70 PCR.pidtemp= 50,0.1,0,300,25,25 @19:41:42.73 PCR.caltemp= 2.7073,0.0373,0 @19:41:42.76 All dwarf controllers running firmware version 3.75 @19:41:42.86 Cleanly exited at 19:37:58CEST26-Aug-18 @19:41:42.89 Loading /home/esp/esp2/mission/18tun.rb mission @19:41:42.93 RuntimeError in MAIN -- Mission's startTube should be 3, not 2 @19:41:42.95 Waiting to Resume from Checkpoint "Loading /home/esp/esp2/mission/18tun.rb mission" @19:41:42.98 BadNews.email "Mission's startTube should be 3, not 2",Subject: "RuntimeError in MAIN" @19:41:43.51 Session Begun @19:41:48.20 -> showlog 100 @19:42:18.11 -> abort! "fixing starttube" @19:42:18.12 CmdServer::Client::Error in bkha1 -- Aborting Mission: fixing starttube @19:42:18.17 BadNews.email "fixing starttube",Subject: "18tun ABORTED!" @19:42:26.59 Session Ended @19:42:47.07CEST26-Aug-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @19:42:48.77 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @19:42:49.03 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @19:42:53.32 Polling Can every 10:00 @19:42:55.47 SPE.coast @19:42:55.54 SPE.configure SPEconfig @19:42:55.62 AS.reconfigure ASconfig @19:42:55.66 AS.hold @19:42:55.70 AS.configure ASconfig @19:42:55.79 SS.reconfigure SSconfig @19:42:55.83 SS.hold @19:42:55.87 SS.configure SSconfig @19:42:55.96 CC.reconfigure CCconfig @19:42:56.00 CC.hold @19:42:56.05 CC.configure CCconfig @19:42:56.13 CS.reconfigure CSconfig @19:42:56.17 CS.hold @19:42:56.29 CS.configure CSconfig @19:42:57.05 PC.reconfigure PCconfig @19:42:57.09 PC.hold @19:42:57.13 PC.configure PCconfig @19:42:57.21 PS.configure PSconfig @19:42:57.31 Forearm.reconfigure LooseForearm @19:42:57.36 Forearm.hold @19:42:57.41 Forearm.configure LooseForearm @19:42:57.49 Elbow.reconfigure LooseElbow @19:42:57.53 Elbow.hold @19:42:57.59 Elbow.configure LooseElbow @19:42:57.77 SC.reconfigure SCloose @19:42:57.84 SC.hold @19:42:57.94 SC.configure SCloose @19:42:58.10 SE.reconfigure SEconfig @19:42:58.18 SE.hold @19:42:58.26 SE.configure SEconfig @19:42:58.34 CH.coast @19:42:58.43 CH.configure CHconfig @19:42:58.46 PH.coast @19:42:58.51 PH.configure PHconfig @19:42:58.54 PCR.off @19:42:58.57 PCR.pidtemp= 50,0.1,0,300,25,25 @19:42:58.60 PCR.caltemp= 2.7073,0.0373,0 @19:42:58.63 All dwarf controllers running firmware version 3.75 @19:42:58.75 Loading /home/esp/esp2/mission/18tun.rb mission @19:42:58.82 Preparing to sleep until +0:00:15 @19:42:58.83 GoodNews.email "Starting 18tun w/pucks in tube 2\n{}\nCan@19:42:53, 16.4C, 41% humidity, 14.6psia, 12.016V, 0.645A, 0.638A avg, 7.75W",Subject: "18tun begun" @19:42:58.90 AS.coast @19:42:58.92
Asleep til +0:00:15 @19:42:58.95 Continuing at 19:43 @19:42:58.99 SS.coast @19:42:59.14 CC.coast @19:42:59.22 CS.coast @19:42:59.28 PC.coast @19:42:59.34 Forearm.coast @19:42:59.40 Elbow.coast @19:42:59.50 SC.coast @19:42:59.56 SE.coast @19:42:59.84 Session Begun @19:43:00.01 Gate.power :core,:OFF @19:43:00.04 Gate.power :raw,:OFF @19:43:00.05 Shutdown @19:43:04.92 -> showlog 100 @19:43:05.59 GoodNews.email "Then, in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:106\n{}\nCan@19:42:59, 16.4C, 41% humidity, 14.6psia, 12.011V, 0.638A, 0.645A avg, 7.67W",Subject: "Asleep until 19:43" @19:43:13.79
Powering core bus @19:43:18.62 Gate.power :raw,:ON @19:43:19.21 SPE.coast @19:43:19.27 SPE.configure SPEconfig @19:43:19.37 AS.configure ASconfig @19:43:19.48 SS.configure SSconfig @19:43:19.58 CC.configure CCconfig @19:43:19.68 CS.configure CSconfig @19:43:19.78 PC.configure PCconfig @19:43:19.88 PS.configure PSconfig @19:43:20.00 Forearm.configure LooseForearm @19:43:20.10 Elbow.configure LooseElbow @19:43:20.24 SC.configure SCloose @19:43:20.34 SE.configure SEconfig @19:43:20.40 CH.coast @19:43:20.46 CH.configure CHconfig @19:43:20.50 PH.coast @19:43:20.57 PH.configure PHconfig @19:43:20.62 PCR.off @19:43:20.66 PCR.pidtemp= 50,0.1,0,300,25,25 @19:43:20.69 PCR.caltemp= 2.7073,0.0373,0 @19:43:20.73 All dwarf controllers running firmware version 3.75 @19:43:20.85 Valve.reset @19:43:21.02 PCR.off @19:43:21.06 PCR.pidtemp= 50,0.1,0,300,25,25 @19:43:21.10 PCR.caltemp= 2.7073,0.0373,0 @19:43:21.19 Intake.select :close @19:43:21.21 Exhaust.select :close @19:43:21.29 CTV.dial :air @19:43:21.43 PSV.dial :air @19:43:21.54 CSV.dial :waste1 @19:43:21.57
Forearm.reconfigure LooseForearm @19:43:21.67 Forearm.home.seek :retract @19:43:22.07 PS.reconfigure PSconfig @19:43:22.19 PS.home.seek 0ml @19:43:22.22 SSV.dial :spit,avoiding: :exhaust @19:43:22.41 CS.reconfigure CSfast @19:43:22.44 SS.reconfigure SSconfig @19:43:22.56 SS.home.seek 0ml @19:43:22.58 CS.home.seek 0ml @19:43:22.69 Hand.close @19:43:22.72
Elbow.jog 1500 @19:43:23.63 Elbow.reconfigure LooseElbow @19:43:23.70 Elbow.home.seek :clear @19:43:27.18 SE.reconfigure SEconfig @19:43:27.24 SE.home.seek :down @19:43:27.86 SC.reconfigure SCsloppy @19:43:27.92 SC.home.seek :initial @19:43:31.53 PS.coast @19:43:38.31
Forearm.seek :retract @19:43:38.40 Elbow.seek :hammer @19:43:39.64 Forearm.reconfigure TightForearm @19:43:39.71 Forearm.seek 5 + 350 counts @19:43:40.80 Forearm.seek :retract @19:43:41.92 PC.reconfigure PCconfig @19:43:41.94
CC.reconfigure CCconfig @19:43:42.03 PC.home.seek :open @19:43:42.06
CC.home.seek :open @19:43:45.37 Phase [coreNEG] @19:43:45.39 GoodNews.email "At 19:43:45 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:106\n{}\nCan@19:43:45, 16.3C, 40% humidity, 14.6psia, 12.011V, 0.645A, 0.645A avg, 7.74W",Subject: "Started 18tun phase coreNEG" @19:43:45.42CEST26-Aug-18
==================> STARTED coreNEG (phase #1) <=== @19:43:45.45 Starting initialPurge @19:43:45.47 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:43:45.56 Hand.open @19:43:46.45 Elbow.reconfigure TightElbow @19:43:46.51 Elbow.seek FlushPuck::Garage @19:43:48.36 Elbow.reconfigure LooseElbow @19:43:48.49 Forearm.reconfigure LooseForearm @19:43:48.61 Forearm.seek :garage @19:43:49.64 Hand.close @19:43:50.57 Forearm.seek :retract @19:43:51.60 Elbow.reconfigure TightElbow @19:43:51.67 Elbow.seek CC @19:43:54.54 Elbow.reconfigure LooseElbow @19:43:54.65 Forearm.reconfigure TightForearm @19:43:54.71 Forearm.seek CC @19:43:55.96 CC.seek :closed @19:44:00.23 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.42 @19:44:00.31 Hand.open @19:44:01.11 Forearm.reconfigure LooseForearm @19:44:01.17 Forearm.seek :retract @19:44:02.16 Flushing the Collection Valve::Manifold @19:44:02.29 CSV.dial CSR @19:44:03.85 Pulling 5ml of CSR.flush @19:44:03.86 CSR.select :flush @19:44:04.00 CS.reconfigure CSconfig @19:44:04.06 CS.seek 5ml @19:44:17.98 delay 5 seconds @19:44:23.00 CSR.select :air @19:44:23.16 CS.reconfigure CSfast @19:44:23.22 CS.seek 7.5ml @19:44:30.60 CSV.dial :waste @19:44:32.11 CS.reconfigure CSconfig @19:44:32.17 CS.seek 0ml @19:44:52.29 Purging external Intake @19:44:52.42 CSV.dial :flush @19:44:52.94 CS.reconfigure CSfast @19:44:53.00 CS.seek 10ml @19:45:19.47 delay 5 seconds @19:45:24.50 CSV.dial CBV @19:45:24.53
CBV.dial CSV @19:45:25.41 CTV.dial :intake @19:45:26.29 Intake.select :open @19:45:26.42 CS.seek 0ml @19:45:53.88 Intake.select :close @19:45:54.01 CTV.dial :air @19:45:54.88 Flushing upper manifold @19:45:55.00 CSV.dial :flush @19:45:55.97 CS.seek 5ml @19:46:09.73 delay 5 seconds @19:46:14.76 CSV.dial :air @19:46:15.35 CS.seek 7ml @19:46:21.44 CSV.dial CBV @19:46:21.47
CBV.dial CSV @19:46:22.68 CTV.dial :air @19:46:22.71 CS.seek 0ml @19:46:41.66 delay 7 seconds @19:46:48.70 CS.seek 3ml @19:46:57.32 CSV.dial :waste @19:46:57.86 CS.seek 0ml @19:47:06.59 CSV.dial :flush @19:47:07.50 CS.seek 3ml @19:47:16.10 delay 5 seconds @19:47:21.13 CSV.dial :air @19:47:21.70 CS.seek 5ml @19:47:27.85 CSV.dial :air @19:47:27.88 CS.seek 0ml @19:47:41.70 delay 7 seconds @19:47:48.73 CS.seek 3ml @19:47:57.45 CSV.dial :waste @19:47:58.31 CS.seek 0ml @19:48:06.92 Garaging FlushPuck in Collection Clamp @19:48:07.00 Elbow.reconfigure TightElbow @19:48:07.06 Elbow.seek CC @19:48:07.32 Elbow.reconfigure LooseElbow @19:48:07.43 Forearm.seek CC @19:48:08.43 Hand.close @19:48:09.19 CC.seek :open @19:48:13.52 Forearm.seek :retract @19:48:14.54 Elbow.reconfigure TightElbow @19:48:14.60 Elbow.seek :garage @19:48:17.74 Elbow.reconfigure LooseElbow @19:48:17.84 Forearm.reconfigure TightForearm @19:48:17.90 Forearm.seek :garage @19:48:19.04 Hand.open @19:48:19.87 Forearm.reconfigure LooseForearm @19:48:19.93 Forearm.seek :retract @19:48:21.04 Starting mfb @19:48:21.05 pcrBAC @19:48:21.10 BACfans.email "Started mfb in tube 2 at 19:48:21CEST26-Aug-18\n{}\nCan@19:43:45, 16.3C, 40% humidity, 14.6psia, 12.011V, 0.645A, 0.645A avg, 7.74W",Subject: "pcrBAC" @19:48:21.16
Loading sh1 puck from tube 2 into Collection Clamp @19:48:21.29 Readying the MFB @19:48:21.35 Homing MFB Syringe @19:48:21.36 AFV.select :holdingCoil @19:48:21.59 ATV.dial :air2 @19:48:21.71
SC.reconfigure SCloose @19:48:21.94 Elbow.reconfigure TightElbow @19:48:22.00 Elbow.seek 2 @19:48:22.20 AS.reconfigure ASconfig @19:48:22.26 AS.home.seek :home @19:48:24.25
Forearm.reconfigure TightForearm @19:48:24.34 Forearm.seek 2 + 700 counts @19:48:25.36 SE.reconfigure SEpick @19:48:25.45 SE.seek beamBreak @19:48:27.79 AFV.select :carrierSolution @19:48:27.91 delay 2 seconds @19:48:28.73
SE.configure SEconfig @19:48:28.89 Elbow.seek 2 @19:48:29.09 Forearm.seek 2 + 300 counts @19:48:29.42 Forearm.reconfigure LooseForearm @19:48:29.49 Hand.close @19:48:29.94 AS.seek 1ml @19:48:30.20 SE.seek :down @19:48:30.42
Forearm.seek :retract @19:48:31.38 Elbow.seek CC @19:48:32.96 SE.coast @19:48:33.50
Elbow.reconfigure LooseElbow @19:48:33.61 Forearm.reconfigure TightForearm @19:48:33.67 Forearm.seek CC @19:48:34.93 CC.seek :closed @19:48:39.28 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.52 @19:48:39.30 Hand.open @19:48:40.13 Forearm.reconfigure LooseForearm @19:48:40.19 Forearm.seek :retract @19:48:41.18 SC.coast @19:48:41.23 skipping prime and sample collection @19:48:41.25 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @19:48:41.27 Intake.select :close @19:48:41.37 CSV.dial :waste @19:48:41.45 CS.seek 0ml @19:48:41.56
Calibrating pressure and temperature sensors @19:48:41.58 CTV.dial :air @19:48:41.61 SSV.dial :spit,avoiding: :exhaust @19:48:41.65 SS.reconfigure SSfast @19:48:41.71 SS.seek 5ml @19:48:41.78 CSV.dial :air @19:48:42.63 CS.seek 10ml @19:48:44.61 SSV.dial CBV @19:48:44.64
CBV.dial SSV @19:48:46.19 delay 3.8 seconds @19:48:50.10 SSV.dial :spit @19:48:51.62 SS.seek 0ml @19:48:54.21 SSV.dial CBV @19:48:55.73 SSV.dial CBV,avoiding: :exhaust @19:48:55.76
CBV.dial SSV @19:48:55.78 CTV.dial :air @19:48:55.81 delay 15 seconds @19:49:10.84 SSV.dial :spit,avoiding: :exhaust @19:49:12.33 CTV.dial CSV,avoiding: :intake @19:49:12.40
SS.reconfigure SSexhale @19:49:12.47 SS.seek 0ml @19:49:12.58 SSV.dial CBV,avoiding: :exhaust @19:49:14.17 CBV.dial between SSV and waste2 @19:49:14.59 CTV.dial CSV,avoiding: :air @19:49:14.62
CSV.dial CTV @19:49:15.16 CS.reconfigure CSpressurize @19:49:15.23 CS.seek 0ml @19:49:22.85 delay 1 second @19:49:23.88 AFV.select :holdingCoil @19:49:23.98 delay 1 second @19:49:25.00 ATV.dial :waste @19:49:26.06 AS.reconfigure ASnorm @19:49:26.12 AS.home.seek :empty @19:49:41.35
CSV.dial :air @19:49:41.89 CS.reconfigure CSfast @19:49:41.95 CS.seek 10ml @19:50:08.23 CSV.dial between air and CTV @19:50:08.60 CS.seek 3.415ml @19:50:18.68 ATV.dial :waste @19:50:18.70 ARV.dial between carrier and air1 @19:50:18.80 ASV.dial :waste1 @19:50:26.35 CTV.dial CSV,avoiding: :air @19:50:26.38
CSV.dial CTV @19:50:26.74 CS.reconfigure CSpressurize @19:50:26.81 CS.seek 0ml @19:50:36.26 CSV.dial :air @19:50:36.80 CS.reconfigure CSfast @19:50:36.86 CS.seek 10ml @19:51:03.23 CSV.dial between air and CTV @19:51:04.03 CS.seek 2.153ml @19:51:25.05 CTV.dial CSV,avoiding: :air @19:51:25.08
CSV.dial CTV @19:51:25.44 CS.reconfigure CSpressurize @19:51:25.51 CS.seek 0ml @19:51:27.52 BubblePoint: Intake @69.9psia, Outlet @14.8psia @19:51:27.53 CBV.dial SSV @19:51:27.93 SS.reconfigure SSconfig @19:51:27.99 SS.seek 10ml @19:51:34.01 delay 15 seconds @19:51:49.14 Evacuated: Intake @28.6psia, Outlet @29.1psia SSV.dial :spit,avoiding: :exhaust @19:51:50.69 SS.reconfigure SSexhale @19:51:50.76 SS.seek 0ml @19:51:56.06 SS.reconfigure SSfast @19:51:56.13 SS.seek 5ml @19:51:58.72 SSV.dial CBV,avoiding: :exhaust @19:51:58.75
CBV.dial SSV @19:52:00.28 CTV.dial :air,avoiding: :intake @19:52:01.83 CSV.dial CTV @19:52:01.87
SS.seek 10ml @19:52:02.87 CSV.dial :air @19:52:03.40 CS.reconfigure CSfast @19:52:03.46 CS.seek 0ml @19:52:04.88
SSV.dial :spit,avoiding: :exhaust @19:52:06.41 SS.reconfigure SSexhale @19:52:06.48 SS.seek 0ml @19:52:08.67 CS.coast @19:52:11.43
SS.reconfigure SSfast @19:52:11.50 SS.seek 5ml @19:52:14.59 SSV.dial CBV,avoiding: :exhaust @19:52:14.62
CBV.dial SSV @19:52:17.17 SSV.dial :spit,avoiding: :exhaust @19:52:18.69 SS.reconfigure SSconfig @19:52:18.75 SS.seek 0ml @19:52:21.76 SS.coast @19:52:21.82 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @19:52:21.83 Intake.select :close @19:52:21.93 CSV.dial :waste @19:52:22.05 SSV.dial CBV,avoiding: :exhaust @19:52:22.08
CBV.dial SSV @19:52:22.82 CS.seek 0ml @19:52:22.92 CSV.dial :air @19:52:23.61
CTV.dial :air @19:52:23.62 delay 15 seconds @19:52:23.79 CS.seek 10ml @19:52:38.65
SSV.dial :spit,avoiding: :exhaust @19:52:40.17 CTV.dial CSV,avoiding: :intake @19:52:40.22
SS.reconfigure SSexhale @19:52:40.31 SS.seek 0ml @19:52:40.80 SSV.dial CBV,avoiding: :exhaust @19:52:50.38 CBV.dial between SSV and waste2 @19:52:50.81 CTV.dial CSV,avoiding: :air @19:52:50.84
CSV.dial CTV @19:52:51.37 CS.reconfigure CSpressurize @19:52:51.44 CS.seek 0ml @19:53:17.54 CSV.dial :air @19:53:18.07 CS.reconfigure CSfast @19:53:18.13 CS.seek 10ml @19:53:44.57 CSV.dial between air and CTV @19:53:44.94 CS.seek 3.383ml @19:54:02.74 CTV.dial CSV,avoiding: :air @19:54:02.76
CSV.dial CTV @19:54:03.13 CS.reconfigure CSpressurize @19:54:03.20 CS.seek 0ml @19:54:12.57 CSV.dial :air @19:54:13.12 CS.reconfigure CSfast @19:54:13.18 CS.seek 10ml @19:54:39.45 CSV.dial between air and CTV @19:54:40.24 CS.seek 2.14ml @19:55:01.27 CTV.dial CSV,avoiding: :air @19:55:01.30
CSV.dial CTV @19:55:01.66 CS.reconfigure CSpressurize @19:55:01.73 CS.seek 0ml @19:55:03.80 BubblePoint: Intake @70.5psia, Outlet @15.6psia @19:55:03.81 CBV.dial SSV @19:55:04.19 SS.reconfigure SSconfig @19:55:04.25 SS.seek 10ml @19:55:10.29 delay 15 seconds @19:55:25.42 Evacuated: Intake @28.9psia, Outlet @29.5psia SSV.dial :spit,avoiding: :exhaust @19:55:26.95 SS.reconfigure SSexhale @19:55:27.02 SS.seek 0ml @19:55:32.06 SS.reconfigure SSfast @19:55:32.13 SS.seek 5ml @19:55:34.98 SSV.dial CBV,avoiding: :exhaust @19:55:35.01
CBV.dial SSV @19:55:36.53 CTV.dial :air,avoiding: :intake @19:55:38.09 CSV.dial CTV @19:55:38.13
SS.seek 10ml @19:55:39.15 CSV.dial :air @19:55:39.68 CS.reconfigure CSfast @19:55:39.74 CS.seek 0ml @19:55:41.16
SSV.dial :spit,avoiding: :exhaust @19:55:42.67 SS.reconfigure SSexhale @19:55:42.74 SS.seek 0ml @19:55:44.76 CS.coast @19:55:47.76
SS.reconfigure SSfast @19:55:47.84 SS.seek 5ml @19:55:50.69 SSV.dial CBV,avoiding: :exhaust @19:55:50.72
CBV.dial SSV @19:55:53.26 SSV.dial :spit,avoiding: :exhaust @19:55:54.78 SS.reconfigure SSconfig @19:55:54.85 SS.seek 0ml @19:55:58.04 SS.coast @19:55:58.10 Lysing: Adding 1.5ml lysis CBV.dial between CSV and waste1,avoiding: :DBV @19:55:59.84 CTV.dial CSV @19:56:01.36 CSV.dial CSR @19:56:02.26 Pulling 1ml of CSR.lysis @19:56:02.27 CSR.select :lysis @19:56:02.40 CS.reconfigure CSconfig @19:56:02.47 CS.seek 1ml @19:56:06.04 delay 5 seconds @19:56:11.07 CSR.select :air @19:56:11.25 CS.seek 6ml @19:56:24.95 delay 2 seconds @19:56:26.98 CSV.dial :waste1 @19:56:28.48 CS.reconfigure CSfast @19:56:28.54 CS.seek :empty @19:56:44.61 CSV.dial CSR @19:56:46.28 Pulling 1.5ml of CSR.lysis @19:56:46.29 CSR.select :lysis @19:56:46.42 CS.reconfigure CSconfig @19:56:46.49 CS.seek 1.5ml @19:56:51.39 delay 5 seconds @19:56:56.42 CSR.select :air @19:56:56.60 CS.seek 4.5ml @19:57:05.30 delay 2 seconds @19:57:07.33 CSV.dial CTV @19:57:07.82 CS.seek 1.275ml @19:57:17.18 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @19:57:17.20 CH.seek 85C,8:00 @20:01:08.49 CH.hold 5:00,85C @20:06:08.64 Collection Puck Heater at 84.7C after lysing @20:06:08.66 CS.seek 0ml @20:06:13.16 CTV.dial between spare and air,avoiding: :intake @20:06:14.36 CSV.dial :air @20:06:14.88 CS.seek 5ml @20:06:28.71 CSV.dial CTV @20:06:29.25 delay 2 seconds @20:06:31.27 CSV.dial :air @20:06:31.80 delay 2 seconds @20:06:33.82 CSV.dial CTV @20:06:34.33 delay 2 seconds @20:06:36.35 CSV.dial :air @20:06:36.89 delay 2 seconds @20:06:38.91 CS.seek 0ml @20:06:52.93 Recovering Lysate into Collection syringe @20:06:52.94 CSV.dial CBV @20:06:54.13 CBV.dial CSV @20:06:54.56 CS.seek 5ml @20:07:08.43 delay 5 seconds @20:07:13.46 CTV.dial :air @20:07:14.05 CS.seek 7.001ml @20:07:20.15 delay 5 seconds @20:07:25.18 CSV.dial :air2 @20:07:25.69 CS.seek 10ml @20:07:34.40 CSV.dial between air and CTV,avoiding: CBV @20:07:35.74 Lysing Complete @20:07:36.28 Storing sh1 puck in Collection Clamp to tube 1 @20:07:36.46 SC.reconfigure SCtight @20:07:36.52 SC.seek 1 @20:07:38.69 SC.reconfigure SCloose @20:07:38.91 Elbow.reconfigure TightElbow @20:07:38.97 Elbow.seek 1 @20:07:40.84 Forearm.reconfigure TightForearm @20:07:40.91 Forearm.seek 1 + 700 counts @20:07:41.97 SE.reconfigure SEpick @20:07:42.07 SE.seek beamBreak @20:07:45.07 SE.configure SEconfig @20:07:45.14 SE.seek :place @20:07:45.64 Forearm.reconfigure LooseForearm @20:07:45.70 Forearm.seek :retract @20:07:46.62 Elbow.seek CC @20:07:48.32 Elbow.reconfigure LooseElbow @20:07:48.43 Forearm.seek CC @20:07:49.41 Hand.close @20:07:50.21 CC.seek :open @20:07:54.71 Forearm.seek :retract @20:07:55.71 Elbow.reconfigure TightElbow @20:07:55.77 Elbow.seek 1 @20:07:57.70 Elbow.reconfigure LimpElbow @20:07:57.76 Forearm.seek 1 @20:07:58.80 Elbow.hold @20:07:58.88 Elbow.reconfigure TightElbow @20:07:58.94 Hand.open @20:07:59.82 Forearm.force -20 @20:07:59.88 Forearm.seek 1 + 326 counts @20:08:00.18 Forearm.reconfigure TightForearm @20:08:00.25 Forearm.seek 1 + 700 counts @20:08:00.66 SE.seek :down @20:08:01.79
Forearm.reconfigure LooseForearm @20:08:01.86 Forearm.seek :retract @20:08:03.46 SE.coast @20:08:03.51
SC.coast @20:08:03.56 Tranferring lysate to PRV @20:08:03.57 CSV.dial :air @20:08:03.92 CS.seek 10ml @20:08:04.00 CSV.dial between air and CTV @20:08:04.36 AIV.dial :bypass @20:08:04.45 Beginning samplePassC @20:08:04.46 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:08:04.55 Hand.open @20:08:04.95 Elbow.seek FlushPuck::Garage @20:08:07.03 Elbow.reconfigure LooseElbow @20:08:07.14 Forearm.seek :garage @20:08:08.17 Hand.close @20:08:09.07 Forearm.seek :retract @20:08:10.57 Elbow.reconfigure TightElbow @20:08:10.63 Elbow.seek CC @20:08:13.62 Elbow.reconfigure LooseElbow @20:08:13.73 Forearm.reconfigure TightForearm @20:08:13.80 Forearm.seek CC @20:08:15.03 CC.seek :closed @20:08:19.30 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.37 @20:08:19.39 Hand.open @20:08:20.14 Forearm.reconfigure LooseForearm @20:08:20.20 Forearm.seek :retract @20:08:21.22 AWV.select :air @20:08:21.24 AIV.dial :bypass @20:08:21.26 PSV.dial PSR @20:08:22.83 Pulling 1.5ml of PSR.flush @20:08:22.84 PSR.select :flush @20:08:22.99 PS.seek 1.5ml @20:08:39.64 delay 5 seconds @20:08:44.66 PSV.dial :waste1 @20:08:45.17 PS.seek 0ml @20:09:01.51 PSV.dial PSR @20:09:02.02 PSR.select :air @20:09:02.16 PS.seek 2.5ml @20:09:29.05 PRV.dial CBV @20:09:29.08
CBV.dial PRV @20:09:29.98 PSV.dial :mixloop @20:09:31.15 CTV.dial :air,avoiding: :intake @20:09:31.17 PTV.dial :PRVmixing @20:09:31.28 PS.seek 0ml @20:09:58.20 PSV.dial :air @20:09:58.72 PS.seek 2.5ml @20:10:25.57 PSV.dial :mixloop @20:10:26.10 PS.seek 0ml @20:10:52.95 PSV.dial :air @20:10:53.47 PS.seek 2.5ml @20:11:20.35 PSV.dial :mixloop @20:11:20.88 PS.seek 0ml @20:11:47.80 Garaging FlushPuck in Collection Clamp @20:11:47.88 Elbow.reconfigure TightElbow @20:11:47.94 Elbow.seek CC @20:11:48.20 Elbow.reconfigure LooseElbow @20:11:48.31 Forearm.seek CC @20:11:49.28 Hand.close @20:11:50.09 CC.seek :open @20:11:54.48 Forearm.seek :retract @20:11:55.50 Elbow.reconfigure TightElbow @20:11:55.56 Elbow.seek :garage @20:11:58.61 Elbow.reconfigure LooseElbow @20:11:58.72 Forearm.reconfigure TightForearm @20:11:58.78 Forearm.seek :garage @20:11:59.94 Hand.open @20:12:00.77 Forearm.reconfigure LooseForearm @20:12:00.83 Forearm.seek :retract @20:12:01.91 Connecting PS to CS to transfer sample @20:12:01.93 CBV.dial AIV @20:12:02.40 AIV.dial :bypass @20:12:02.43 PSV.dial :mixloop @20:12:02.45 PTV.dial :PRVmixing @20:12:02.47 PRV.dial AIV @20:12:02.50 CTV.dial :air,avoiding: :intake @20:12:02.53 Loading lyfil puck from tube 2 into Collection Clamp @20:12:02.77 SC.reconfigure SCtight @20:12:02.83 SC.seek 2 @20:12:05.02 SC.reconfigure SCloose @20:12:05.23 Elbow.reconfigure TightElbow @20:12:05.29 Elbow.seek 2 @20:12:07.34 Forearm.reconfigure TightForearm @20:12:07.41 Forearm.seek 2 + 700 counts @20:12:08.41 SE.reconfigure SEpick @20:12:08.51 SE.seek beamBreak @20:12:11.65 SE.configure SEconfig @20:12:11.81 Elbow.seek 2 @20:12:12.17 Forearm.seek 2 + 300 counts @20:12:12.47 Forearm.reconfigure LooseForearm @20:12:12.53 Hand.close @20:12:13.35 SE.seek :down @20:12:13.56
Forearm.seek :retract @20:12:14.56 Elbow.seek CC @20:12:16.25 Elbow.reconfigure LooseElbow @20:12:16.38 SE.coast @20:12:16.40
Forearm.reconfigure TightForearm @20:12:16.49 Forearm.seek CC @20:12:17.74 CC.seek :closed @20:12:21.98 Collection Clamp onSpring/offSpring for 5/30 samples, on/off torque 2.52 @20:12:22.00 Hand.open @20:12:22.78 Forearm.reconfigure LooseForearm @20:12:22.83 Forearm.seek :retract @20:12:23.82 SC.coast @20:12:23.87 CTV.dial CSV,avoiding: :intake @20:12:25.40 CSV.dial CTV @20:12:25.76 CS.seek 5ml @20:12:39.50 delay 1:20 @20:13:59.53 CS.seek 2.5ml @20:14:06.93 delay 40 seconds @20:14:46.96 CS.seek 0ml @20:14:54.31 delay 1:00 @20:15:54.34 PSV.dial :air @20:15:54.85 PS.seek 2.5ml @20:16:21.73 PSV.dial :mixloop @20:16:22.25 delay 3 seconds @20:16:25.27 PSV.dial :air @20:16:25.79 delay 2 seconds @20:16:27.81 PSV.dial :mixloop @20:16:28.33 delay 3 seconds @20:16:31.35 PSV.dial :waste1 @20:16:32.86 PS.seek :empty @20:16:59.82 CBV.dial :waste1 @20:17:00.35 delay 5 seconds @20:17:05.37 CBV.dial AIV @20:17:05.90 delay 10 seconds @20:17:15.93 CBV.dial :waste1 @20:17:16.45 delay 10 seconds @20:17:26.49 CBV.dial AIV @20:17:27.48 delay 10 seconds @20:17:37.51 CTV.dial CSV,avoiding: :intake @20:17:37.52 CBV.dial between AIV and waste1 @20:17:37.92 CSV.dial :air @20:17:38.45 CS.seek 10ml @20:18:04.94 CSV.dial CTV @20:18:05.46 CS.seek 0ml @20:18:32.13 CBV.dial :waste1 @20:18:32.48 delay 10 seconds @20:18:42.51 CTV.dial :air,avoiding: :intake @20:18:44.09 Storing lyfil puck in Collection Clamp to tube 1 @20:18:44.27 SC.reconfigure SCtight @20:18:44.33 SC.seek 1 @20:18:46.47 SC.reconfigure SCloose @20:18:46.68 Elbow.reconfigure TightElbow @20:18:46.74 Elbow.seek 1 @20:18:48.65 Forearm.reconfigure TightForearm @20:18:48.72 Forearm.seek 1 + 700 counts @20:18:49.80 SE.reconfigure SEpick @20:18:49.95 SE.seek beamBreak @20:18:53.10 SE.configure SEconfig @20:18:53.26 Elbow.reconfigure LimpElbow @20:18:53.32 Forearm.reconfigure LooseForearm @20:18:53.39 Forearm.seek 1 @20:18:53.70 Elbow.hold @20:18:53.77 Elbow.reconfigure TightElbow @20:18:53.88 SE.seek 0.03 Pucks below Top Plate @20:18:54.31 Forearm.force -20 @20:18:54.36 Forearm.seek 1 + 337 counts @20:18:54.68 Forearm.reconfigure TightForearm @20:18:54.74 Forearm.seek 1 + 700 counts @20:18:54.99 SE.seek 1.03 Pucks below Top Plate @20:18:55.57 Forearm.reconfigure LooseForearm @20:18:55.63 Forearm.seek :retract @20:18:56.55 Elbow.seek CC @20:18:58.24 Elbow.reconfigure LooseElbow @20:18:58.35 Forearm.seek CC @20:18:59.31 Hand.close @20:19:00.10 CC.seek :open @20:19:04.56 Forearm.seek :retract @20:19:05.55 Elbow.reconfigure TightElbow @20:19:05.61 Elbow.seek 1 @20:19:07.50 Elbow.reconfigure LimpElbow @20:19:07.57 Forearm.seek 1 @20:19:08.60 Elbow.hold @20:19:08.69 Elbow.reconfigure TightElbow @20:19:08.74 Hand.open @20:19:09.58 Forearm.force -20 @20:19:09.64 Forearm.seek 1 + 328 counts @20:19:09.94 Forearm.reconfigure TightForearm @20:19:10.01 Forearm.seek 1 + 700 counts @20:19:10.75 SE.seek :down @20:19:11.88
Forearm.reconfigure LooseForearm @20:19:11.94 Forearm.seek :retract @20:19:13.45 SE.coast @20:19:13.50
SC.coast @20:19:13.55 PSV.dial :air @20:19:14.90 PS.seek 0.499ml @20:19:20.89 delay 3 seconds @20:19:23.92 PSV.dial :mixloop @20:19:24.43 PRV.dial :air @20:19:24.96 delay 3 seconds @20:19:27.98 PRV.dial CBV @20:19:28.50 CBV.dial AIV @20:19:29.03 PRV.dial between CBV and air @20:19:29.40 PRV.dial :air @20:19:29.79 PS.reconfigure PSslow @20:19:29.85 PS.seek 0.599ml @20:19:32.42 PRV.dial :waste2 @20:19:33.27 PS.reconfigure PSconfig @20:19:33.33 PS.seek :empty @20:19:40.40 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:19:40.48 Hand.open @20:19:40.83 Elbow.seek FlushPuck::Garage @20:19:42.95 Elbow.reconfigure LooseElbow @20:19:43.06 Forearm.seek :garage @20:19:44.10 Hand.close @20:19:44.96 Forearm.seek :retract @20:19:46.02 Elbow.reconfigure TightElbow @20:19:46.08 Elbow.seek CC @20:19:49.07 Elbow.reconfigure LooseElbow @20:19:49.18 Forearm.reconfigure TightForearm @20:19:49.24 Forearm.seek CC @20:19:50.47 CC.seek :closed @20:19:54.72 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.46 @20:19:54.79 Hand.open @20:19:55.65 Forearm.reconfigure LooseForearm @20:19:55.72 Forearm.seek :retract @20:19:56.74 Clearing PML for modified lysate @20:19:56.75 CTV.dial :air,avoiding: :intake @20:19:56.78 PRV.dial :waste2 @20:19:56.80 PTV.dial :PRV,avoiding: :puck @20:19:57.66 delay 2 seconds @20:19:59.68 PTV.dial :PRVmixing,avoiding: :puck @20:20:00.51 delay 2 seconds @20:20:02.54 PSV.dial :PML @20:20:02.55 delay 2 seconds @20:20:04.58 PRV.dial :air @20:20:05.41 delay 2 seconds @20:20:07.48 PS.seek 1.499ml @20:20:23.95 delay 5 seconds @20:20:28.98 PS.seek 2.5ml @20:20:40.26 PSV.dial :air @20:20:40.79 PS.seek 0ml @20:21:07.77 mixing lysate with speDil @20:21:08.21 PSV.dial :PML @20:21:08.76 CBV.dial :PRV @20:21:08.78 PRV.dial :air @20:21:08.81 delay 2 seconds @20:21:10.84 PRV.dial :speDil @20:21:11.37 delay 2 seconds @20:21:13.44 PS.reconfigure PSslow1 @20:21:13.50 PS.seek 0.225ml @20:21:37.17 delay 5 seconds @20:21:42.20 PRV.dial CBV @20:21:43.07 delay 2 seconds @20:21:45.14 PS.seek 0.475ml @20:22:10.83 delay 5 seconds @20:22:15.86 PRV.dial :air @20:22:16.40 delay 2 seconds @20:22:18.42 PS.seek 0.965ml @20:23:08.51 delay 10 seconds @20:23:18.54 PRV.dial CBV @20:23:19.06 CTV.dial :air,avoiding: :intake @20:23:19.08 PSV.dial :air @20:23:19.62 PS.reconfigure PSconfig @20:23:19.68 PS.seek 2.5ml @20:23:36.42 delay 3 seconds @20:23:39.45 PSV.dial PTV @20:23:40.30 PTV.dial :PRV,avoiding: :puck @20:23:41.15 PS.seek 0ml @20:24:08.11 PSV.dial :air @20:24:08.96 PS.seek 2.5ml @20:24:35.61 delay 3 seconds @20:24:38.64 PSV.dial PTV @20:24:39.47 PTV.dial :PRV,avoiding: :puck @20:24:39.50 PS.seek 0ml @20:25:06.42 Pushing modified lysate into AIV Injection Loop @20:25:06.43 CBV.dial AIV @20:25:06.45 AIV.dial :bypass @20:25:06.48 PRV.dial CBV @20:25:06.50 PSV.dial :air @20:25:07.35 PS.seek 2.5ml @20:25:34.23 delay 3 seconds @20:25:37.26 PSV.dial :PML @20:25:37.78 PTV.dial :PRVmixing,avoiding: :puck @20:25:38.69 PS.reconfigure PSslow1 @20:25:38.75 PS.seek 1.74ml @20:26:56.24 delay 5 seconds @20:27:01.27 AIV.dial :sample @20:27:01.91 AWV.select :waste @20:27:02.09 PS.reconfigure PSslow2 @20:27:02.15 PS.seek 1.22ml @20:28:49.83 delay 15 seconds @20:29:04.86 AIV.dial :bypass @20:29:05.50 PS.reconfigure PSconfig @20:29:05.56 PS.seek 0ml @20:29:18.99 PSV.dial :air @20:29:19.51 PS.seek 2.5ml @20:29:46.24 PSV.dial :PML @20:29:46.76 PS.seek 0ml @20:30:13.46 AIV.dial :coilToMFB @20:30:13.48 AWV.select :air @20:30:13.66 Extracting Nucleic Acid from AIV @20:30:13.69 SPE.hold 2:46:40,55C @20:30:13.72
Beginning samplePassD @20:30:13.81 ASV.dial :SPEdelivery @20:30:13.90
AWV.select :air @20:30:13.91 AIV.dial :bypass @20:30:13.93 PSV.dial PSR @20:30:14.25 delay 20 seconds @20:30:15.24
Pulling 1.5ml of PSR.killMFB @20:30:15.25 PSR.select :killMFB @20:30:15.40 PS.seek 1.5ml @20:30:32.59 delay 5 seconds @20:30:34.28 primeLysateINT @20:30:34.29 AWV.select :air @20:30:34.31 AIV.dial :coilToMFB @20:30:34.33 ATV.dial AIV @20:30:36.24 AS.reconfigure ASslow1 @20:30:36.30 AS.seek 0.299ml @20:30:37.61
PSV.dial :waste1 @20:30:38.15 PS.seek 0ml @20:30:55.68 PSV.dial PSR @20:30:56.22 PSR.select :air @20:30:56.36 PS.seek 2.5ml @20:31:24.31 PRV.dial CBV @20:31:24.34
CBV.dial PRV @20:31:24.36 PSV.dial :mixloop @20:31:25.59 CTV.dial :air,avoiding: :intake @20:31:25.60 PTV.dial :PRVmixing @20:31:25.63 PS.seek 0ml @20:31:36.90 delay 10 seconds @20:31:46.94 ATV.dial :air2 @20:31:47.85 AS.seek 0.329ml @20:31:53.64
PSV.dial PSR @20:31:54.17 ATV.dial :waste2 @20:31:54.79 AS.reconfigure ASnorm @20:31:54.85 AS.seek :empty @20:31:54.99
Pulling 1.5ml of PSR.flush @20:31:55.00 PSR.select :flush @20:31:55.15 PS.seek 1.5ml @20:32:11.88 Lysate primed! @20:32:11.89 AFV.select :holdingCoil @20:32:11.91 ATV.dial :air2 @20:32:12.28
delay 5 seconds @20:32:12.59 AS.reconfigure ASslow1 @20:32:12.66 AS.seek 0.069ml @20:32:17.30
PSV.dial :waste1 @20:32:17.84 PS.seek 0ml @20:32:27.14 delay 3 seconds @20:32:30.17 ATV.dial :AIV @20:32:31.06 AS.seek 0.469ml @20:32:34.93
PSV.dial PSR @20:32:35.47 PSR.select :air @20:32:35.61 PS.seek 2.5ml @20:33:03.62 PRV.dial CBV @20:33:03.65
CBV.dial PRV @20:33:03.67 PSV.dial :mixloop @20:33:04.90 CTV.dial :air,avoiding: :intake @20:33:04.91 PTV.dial :PRVmixing @20:33:04.94 PS.seek 0ml @20:33:33.02 PSV.dial PSR @20:33:34.30 Pulling 1.5ml of PSR.flush @20:33:34.31 PSR.select :flush @20:33:34.46 PS.seek 1.5ml @20:33:51.47 delay 5 seconds @20:33:51.79 delay 5 seconds @20:33:56.49
PSV.dial :waste1 @20:33:56.86 Solid Phase Extraction Heater at 47C @20:33:56.87 ATV.dial ASV @20:33:57.14
PS.seek 0ml @20:33:57.29 ASV.dial :SPEdelivery @20:33:57.38 AS.seek 0.041ml @20:34:14.15
PSV.dial PSR @20:34:14.66 PSR.select :air @20:34:14.80 PS.seek 2.5ml @20:34:42.41 PRV.dial CBV @20:34:42.43
CBV.dial PRV @20:34:42.46 PSV.dial :mixloop @20:34:43.80 CTV.dial :air,avoiding: :intake @20:34:43.81 PTV.dial :PRVmixing @20:34:43.84 PS.seek 0ml @20:35:11.27 PSV.dial PSR @20:35:12.63 Pulling 1.5ml of PSR.flush @20:35:12.64 PSR.select :flush @20:35:12.79 PS.seek 1.5ml @20:35:23.75 delay 10 seconds @20:35:29.37
delay 5 seconds @20:35:33.78 rinseLysateLineINT @20:35:33.79 AFV.select :carrierSolution @20:35:33.92 delay 2 seconds @20:35:34.39
PSV.dial :waste1 @20:35:34.92 PS.seek 0ml @20:35:35.94 AS.reconfigure ASconfig @20:35:36.00 AS.seek 1ml @20:35:51.58
PSV.dial PSR @20:35:52.12 PSR.select :air @20:35:52.26 PS.seek 2.5ml @20:36:19.44 PRV.dial CBV @20:36:19.46
CBV.dial PRV @20:36:19.88 PSV.dial :mixloop @20:36:21.14 CTV.dial :air,avoiding: :intake @20:36:21.15 PTV.dial :PRVmixing @20:36:21.18 PS.reconfigure PSslow @20:36:21.26 PS.seek 1.2ml @20:36:31.40 delay 1 second @20:36:32.43 AFV.select :holdingCoil @20:36:32.53 delay 1 second @20:36:33.55 ATV.dial AIV @20:36:34.23 AIV.dial :coilToMFB @20:36:34.24 AWV.select :waste @20:36:34.37 AS.reconfigure ASslow0 @20:36:34.43 AS.seek 0ml @20:36:48.16
PSV.dial :waste1 @20:36:49.69 PS.reconfigure PSconfig @20:36:49.76 PS.seek 0ml @20:37:03.21 PSV.dial :air @20:37:04.41 PS.seek 2.5ml @20:37:31.59 PSV.dial :mixloop @20:37:32.09 PS.reconfigure PSslow @20:37:32.15 PS.seek 0ml @20:38:15.36 ATV.dial :air2 @20:38:16.04 AS.reconfigure ASslow1 @20:38:16.11 AS.seek 0.05ml @20:38:23.34
PSV.dial :air @20:38:23.86 PS.reconfigure PSconfig @20:38:23.92 PS.seek 2.5ml @20:38:26.53 ATV.dial :AIV @20:38:27.38 AS.seek 0.01ml @20:38:35.69 ATV.dial :waste @20:38:37.38 AS.seek :empty @20:38:39.81 ethanol wash @20:38:39.82 ATV.dial :air2 @20:38:41.12 AS.reconfigure ASslow2 @20:38:41.18 AS.seek 0.009ml @20:38:50.77
PSV.dial :mixloop @20:38:51.30 PS.reconfigure PSslow @20:38:51.36 PS.seek 0ml @20:38:51.62 delay 3 seconds @20:38:54.65 Pulling 0.4ml of Ethanol Wash @20:38:54.66 ATV.dial :ethanol @20:38:55.50 AS.reconfigure ASslow1 @20:38:55.56 AS.seek 0.409ml @20:39:42.69
Cleaning Collection Syringe @20:39:42.70 CSV.dial CSR @20:39:43.26 Pulling 4ml of CSR.flush @20:39:43.27 CSR.select :flush @20:39:43.42 CS.seek 4ml @20:39:54.78 delay 5 seconds @20:39:59.80 CSR.select :air @20:39:59.93 CS.seek 10ml @20:40:16.31 CSV.dial CTV @20:40:16.33
CTV.dial CSV @20:40:16.46 delay 3 seconds @20:40:17.92
CBV.dial :waste1 @20:40:18.47 CS.seek 0ml @20:40:19.48 ATV.dial :air1 @20:40:19.87 delay 3 seconds @20:40:22.95 AS.seek 0.434ml @20:40:28.32 delay 3 seconds @20:40:31.35 ATV.dial :ethanol @20:40:31.97 AS.seek 0.419ml @20:40:35.28 delay 3 seconds @20:40:38.31 ATV.dial ASV @20:40:39.66 AS.seek 0.092ml @20:40:45.24
CSV.dial CSR @20:40:45.94 Pulling 4ml of CSR.flush @20:40:45.95 CSR.select :flush @20:40:46.10 CS.seek 4ml @20:40:57.31 delay 5 seconds @20:41:02.33 CSR.select :air @20:41:02.47 CS.seek 10ml @20:41:18.81 CSV.dial CTV @20:41:18.84
CTV.dial CSV @20:41:19.36 CBV.dial :waste1 @20:41:19.38 CS.seek 0ml @20:41:45.67 delay 3 seconds @20:41:46.05
Garaging FlushPuck in Collection Clamp @20:41:46.59 Elbow.reconfigure TightElbow @20:41:46.66 Elbow.seek CC @20:41:46.92 Elbow.reconfigure LooseElbow @20:41:47.03 Forearm.seek CC @20:41:48.05 Hand.close @20:41:48.70 ATV.dial :waste2 @20:41:48.87
CC.seek :open @20:41:49.48 AS.reconfigure ASconfig @20:41:49.54 AS.seek :empty @20:41:53.23
Forearm.seek :retract @20:41:54.23 Elbow.reconfigure TightElbow @20:41:54.29 Elbow.seek :garage @20:41:55.19 ATV.dial :air2 @20:41:55.60 AS.reconfigure ASslow1 @20:41:55.66 AS.seek 1ml @20:41:57.37
Elbow.reconfigure LooseElbow @20:41:57.48 Forearm.reconfigure TightForearm @20:41:57.54 Forearm.seek :garage @20:41:58.78 Hand.open @20:41:59.56 Forearm.reconfigure LooseForearm @20:41:59.61 Forearm.seek :retract @20:42:00.68 Skipping Whole Cell Archive @20:42:00.69 Starting finalPurge @20:42:00.72 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:42:00.81 Hand.open @20:42:01.17 Elbow.reconfigure TightElbow @20:42:01.24 Elbow.seek FlushPuck::Garage @20:42:01.37 Elbow.reconfigure LooseElbow @20:42:01.48 Forearm.seek :garage @20:42:02.51 Hand.close @20:42:03.36 Forearm.seek :retract @20:42:04.39 Elbow.reconfigure TightElbow @20:42:04.45 Elbow.seek CC @20:42:07.37 Elbow.reconfigure LooseElbow @20:42:07.48 Forearm.reconfigure TightForearm @20:42:07.55 Forearm.seek CC @20:42:08.79 CC.seek :closed @20:42:13.09 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.43 @20:42:13.19 Hand.open @20:42:14.05 Forearm.reconfigure LooseForearm @20:42:14.12 Forearm.seek :retract @20:42:15.09 Flushing the Collection Valve::Manifold @20:42:15.22 CSV.dial CSR @20:42:15.80 Pulling 5ml of CSR.flush @20:42:15.81 CSR.select :flush @20:42:15.95 CS.seek 5ml @20:42:29.75 delay 5 seconds @20:42:34.77 CSR.select :air @20:42:34.95 CS.reconfigure CSfast @20:42:35.01 CS.seek 7.501ml @20:42:42.36 CSV.dial :waste @20:42:43.89 CS.reconfigure CSconfig @20:42:44.43 CS.seek 0ml @20:43:04.59 Flushing lower manifold's waste1 port @20:43:04.72 CSV.dial :flush @20:43:05.25 CS.reconfigure CSfast @20:43:05.32 CS.seek 5ml @20:43:19.02 delay 5 seconds @20:43:24.05 CSV.dial :air @20:43:24.62 CS.seek 7ml @20:43:30.97 CSV.dial CTV @20:43:31.00
CTV.dial CSV @20:43:31.51 CBV.dial :waste1 @20:43:31.53 CS.seek 0ml @20:43:50.50 Flushing lower manifold's waste2b port @20:43:50.63 CSV.dial :flush @20:43:51.48 CS.seek 5ml @20:44:05.22 delay 5 seconds @20:44:10.24 CSV.dial :air @20:44:10.82 CS.seek 7ml @20:44:16.87 CSV.dial CTV @20:44:16.90
CTV.dial CSV @20:44:17.41 CBV.dial :waste2b @20:44:18.26 CS.seek 0ml @20:44:37.12 Flushing lower manifold's waste1 port @20:44:37.13 CSV.dial CSR @20:44:37.81 Pulling 4ml of CSR.mfbkill @20:44:37.82 CSR.select :mfbkill @20:44:37.97 CS.reconfigure CSconfig @20:44:38.03 CS.seek 4ml @20:44:49.22 delay 5 seconds @20:44:54.24 CSR.select :air @20:44:54.37 CS.reconfigure CSfast @20:44:54.44 CS.seek 10ml @20:45:10.88 CSV.dial CTV @20:45:10.91
CTV.dial CSV @20:45:11.42 CBV.dial :waste1 @20:45:12.28 CS.seek 0ml @20:45:17.31 delay 5 seconds @20:45:22.34 ATV.dial ASV @20:45:23.34 ASV.dial :SPEdelivery @20:45:23.37 AS.seek 0.02ml @20:45:38.78
Flushing lower manifold's waste2b port @20:45:38.79 CSV.dial CSR @20:45:39.38 Pulling 4ml of CSR.mfbkill @20:45:39.39 CSR.select :mfbkill @20:45:39.54 CS.reconfigure CSconfig @20:45:39.60 CS.seek 4ml @20:45:50.78 delay 5 seconds @20:45:55.80 CSR.select :air @20:45:55.92 CS.reconfigure CSfast @20:45:55.99 CS.seek 10ml @20:46:12.28 CSV.dial CTV @20:46:12.31
CTV.dial CSV @20:46:12.84 CBV.dial :waste2b @20:46:13.70 CS.seek 0ml @20:46:40.44 Killing upper manifold CSV.dial CSR @20:46:41.02 Pulling 4ml of CSR.mfbkill @20:46:41.03 CSR.select :mfbkill @20:46:41.18 CS.reconfigure CSconfig @20:46:41.24 CS.seek 4ml @20:46:52.44 delay 5 seconds @20:46:57.46 CSR.select :air @20:46:57.59 CS.reconfigure CSfast @20:46:57.66 CS.seek 10ml @20:47:13.91 CSV.dial CBV @20:47:13.94
CBV.dial CSV @20:47:15.16 CTV.dial :air @20:47:16.71 CS.seek 0ml @20:47:43.46 delay 7 seconds @20:47:50.50 CS.seek 3ml @20:47:59.15 CSV.dial :waste @20:47:59.66 CS.seek 0ml @20:48:08.31 Flushing upper manifold @20:48:08.44 CSV.dial :flush @20:48:08.96 CS.seek 5ml @20:48:22.75 delay 5 seconds @20:48:27.78 CSV.dial :air @20:48:28.48 CS.seek 7ml @20:48:34.66 CSV.dial CBV @20:48:34.69
CBV.dial CSV @20:48:35.86 CTV.dial :air @20:48:35.88 CS.seek 0ml @20:48:40.72 delay 5 seconds @20:48:45.75 ATV.dial :air2 @20:48:46.82 AS.seek 0.52ml @20:48:54.62
delay 7 seconds @20:49:01.66 CS.seek 3ml @20:49:10.34 CSV.dial :waste @20:49:10.86 CS.seek 0ml @20:49:19.63 CSV.dial :flush @20:49:20.14 CS.seek 3ml @20:49:28.84 delay 5 seconds @20:49:33.87 CSV.dial :air @20:49:34.45 CS.seek 5ml @20:49:40.68 CSV.dial :air @20:49:40.71 CS.seek 0ml @20:49:54.37 delay 7 seconds @20:50:01.42 CS.seek 3ml @20:50:10.09 CSV.dial :waste @20:50:10.96 CS.seek 0ml @20:50:19.54 Flushing lower manifold's waste1 port @20:50:19.67 CSV.dial :flush @20:50:20.21 CS.seek 5ml @20:50:27.60 delay 5 seconds @20:50:32.63 ATV.dial ASV @20:50:33.63 ASV.dial :wasteR @20:50:33.90
delay 5 seconds @20:50:34.11 AS.seek 0.3ml @20:50:38.93
CSV.dial :air @20:50:39.49 CS.seek 7ml @20:50:45.75 CSV.dial CTV @20:50:45.78
CTV.dial CSV @20:50:47.37 CBV.dial :waste1 @20:50:47.89 CS.seek 0ml @20:51:06.54 Flushing lower manifold's waste2b port @20:51:06.67 CSV.dial :flush @20:51:07.52 CS.seek 5ml @20:51:18.64 delay 20 seconds @20:51:21.15
delay 5 seconds @20:51:26.18 CSV.dial :air @20:51:26.75 CS.seek 7ml @20:51:32.76 CSV.dial CTV @20:51:32.79
CTV.dial CSV @20:51:33.30 CBV.dial :waste2b @20:51:34.16 CS.seek 0ml @20:51:38.67 AS.seek 0.07ml @20:51:52.94
Garaging FlushPuck in Collection Clamp @20:51:53.03 Elbow.reconfigure TightElbow @20:51:53.08 Elbow.seek CC @20:51:53.34 Elbow.reconfigure LooseElbow @20:51:53.45 Forearm.seek CC @20:51:54.43 Hand.close @20:51:55.26 CC.seek :open @20:51:59.65 Forearm.seek :retract @20:52:00.65 Elbow.reconfigure TightElbow @20:52:00.71 Elbow.seek :garage @20:52:03.93 Elbow.reconfigure LooseElbow @20:52:04.04 Forearm.reconfigure TightForearm @20:52:04.09 Forearm.seek :garage @20:52:05.28 Hand.open @20:52:06.57 Forearm.reconfigure LooseForearm @20:52:06.63 Forearm.seek :retract @20:52:07.66 Starting initialPurge @20:52:07.67 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:52:07.76 Hand.open @20:52:08.06 Elbow.reconfigure TightElbow @20:52:08.13 Elbow.seek FlushPuck::Garage @20:52:08.26 Elbow.reconfigure LooseElbow @20:52:08.37 Forearm.seek :garage @20:52:09.41 Hand.close @20:52:10.24 Forearm.seek :retract @20:52:11.27 Elbow.reconfigure TightElbow @20:52:11.33 Elbow.seek CC @20:52:14.31 Elbow.reconfigure LooseElbow @20:52:14.42 Forearm.reconfigure TightForearm @20:52:14.48 Forearm.seek CC @20:52:15.73 CC.seek :closed @20:52:19.99 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.38 @20:52:20.08 Hand.open @20:52:20.93 Forearm.reconfigure LooseForearm @20:52:21.00 Forearm.seek :retract @20:52:21.98 Flushing the Collection Valve::Manifold @20:52:22.11 CSV.dial CSR @20:52:22.68 Pulling 5ml of CSR.flush @20:52:22.69 CSR.select :flush @20:52:22.83 CS.reconfigure CSconfig @20:52:22.89 CS.seek 5ml @20:52:25.19 delay 20 seconds @20:52:36.66
delay 5 seconds @20:52:41.68 CSR.select :air @20:52:41.86 CS.reconfigure CSfast @20:52:41.91 CS.seek 7.5ml @20:52:45.22 ATV.dial :waste2 @20:52:46.00 AS.reconfigure ASconfig @20:52:46.05 AS.seek :empty @20:52:49.25
CSV.dial :waste @20:52:50.42 Elution @20:52:50.43 ATV.dial :air1 @20:52:50.76
CS.reconfigure CSconfig @20:52:50.82 CS.seek 0ml @20:52:51.58 AS.reconfigure ASslow1 @20:52:51.65 AS.seek 0.049ml @20:53:02.00 delay 3 seconds @20:53:05.03 ATV.dial :ElutionWater @20:53:05.72 AS.seek 0.109ml @20:53:10.93
Purging external Intake @20:53:11.07 CSV.dial :flush @20:53:11.58 CS.reconfigure CSfast @20:53:11.64 CS.seek 10ml @20:53:18.03 delay 3 seconds @20:53:21.06 ATV.dial ASV,via: :air2 @20:53:23.20 ASV.dial :SPEelution @20:53:23.80 AFV.select :carrierSolution @20:53:23.96 AS.reconfigure ASnorm @20:53:24.02 AS.seek 0.759ml @20:53:38.18
delay 5 seconds @20:53:43.21 CSV.dial CBV @20:53:43.25
CBV.dial CSV @20:53:44.17 CTV.dial :intake @20:53:45.04 Intake.select :open @20:53:45.17 CS.seek 0ml @20:53:57.06 delay 3 seconds @20:54:00.09 AFV.select :holdingCoil @20:54:00.23 AS.reconfigure ASslow1 @20:54:00.29 AS.seek 0.474ml @20:54:12.47
Intake.select :close @20:54:12.59 CTV.dial :air @20:54:13.94 Flushing upper manifold @20:54:14.07 CSV.dial :flush @20:54:14.91 CS.seek 5ml @20:54:28.62 delay 5 seconds @20:54:33.65 CSV.dial :air @20:54:34.24 CS.seek 7ml @20:54:40.38 CSV.dial CBV @20:54:40.41
CBV.dial CSV @20:54:41.60 CTV.dial :air @20:54:41.62 CS.seek 0ml @20:54:57.88 delay 5 seconds @20:55:00.40
delay 7 seconds @20:55:02.97 AS.reconfigure ASslow2 @20:55:03.03 AS.seek 0.439ml @20:55:07.43
CS.seek 3ml @20:55:16.06 CSV.dial :waste @20:55:16.60 CS.seek 0ml @20:55:25.30 CSV.dial :flush @20:55:25.80 CS.seek 3ml @20:55:34.47 delay 5 seconds @20:55:38.33 delay 1:00 @20:55:39.50
CSV.dial :air @20:55:40.05 CS.seek 5ml @20:55:46.06 CSV.dial :air @20:55:46.09 CS.seek 0ml @20:55:59.94 delay 7 seconds @20:56:06.98 CS.seek 3ml @20:56:15.78 CSV.dial :waste @20:56:16.63 CS.seek 0ml @20:56:25.20 Garaging FlushPuck in Collection Clamp @20:56:25.28 Elbow.reconfigure TightElbow @20:56:25.34 Elbow.seek CC @20:56:25.62 Elbow.reconfigure LooseElbow @20:56:25.73 Forearm.seek CC @20:56:26.72 Hand.close @20:56:27.51 CC.seek :open @20:56:31.90 Forearm.seek :retract @20:56:32.89 Elbow.reconfigure TightElbow @20:56:32.95 Elbow.seek :garage @20:56:36.23 Elbow.reconfigure LooseElbow @20:56:36.34 Forearm.reconfigure TightForearm @20:56:36.39 Forearm.seek :garage @20:56:37.56 Hand.open @20:56:38.38 pushing eluate to holding coil @20:56:38.53
Forearm.reconfigure LooseForearm @20:56:38.56 AS.seek 0.279ml @20:56:38.65
Forearm.seek :retract @20:56:39.69 Completed [coreNEG] begun 19:43:45 @20:56:39.71 Extending 18tun until completion of MFBPCR @20:59:19.66 delay 30 seconds @20:59:49.70 ATV.dial :waste,via: :air3 @20:59:51.43 AS.reconfigure ASconfig @20:59:51.48 AS.seek :empty @21:00:07.88 Parking Bleach in LysateSource line and SPEcolumn @21:00:07.89 ATV.dial :air3 @21:00:08.73 AS.reconfigure ASslow1 @21:00:08.79 AS.seek 0.029ml @21:00:15.19 ATV.dial :ElutionWater @21:00:15.81 AS.seek 0.059ml @21:00:22.21 ATV.dial :air3 @21:00:22.83 AS.seek 0.069ml @21:00:25.13 delay 2 seconds @21:00:27.16 ATV.dial :bleach @21:00:27.63 AS.seek 0.769ml @21:02:48.64 delay 3 seconds @21:02:51.67 ATV.dial :air3 @21:02:52.24 delay 3 seconds @21:02:55.32 AS.seek 0.794ml @21:03:00.59 delay 3 seconds @21:03:03.62 ATV.dial :bleach @21:03:04.25 AS.seek 0.779ml @21:03:07.58 delay 3 seconds @21:03:10.61 ATV.dial :AIV @21:03:12.33 AS.reconfigure ASconfig @21:03:12.40 AS.seek 0.015ml @21:03:56.61 ATV.dial :waste @21:03:58.14 AS.seek :empty @21:03:59.35 ATV.dial :air3 @21:04:00.03 AS.reconfigure ASslow1 @21:04:00.10 AS.seek 0.009ml @21:04:02.39 delay 2 seconds @21:04:04.42 ATV.dial :bleach @21:04:04.89 AS.seek 0.579ml @21:05:59.77 delay 3 seconds @21:06:02.80 ATV.dial :air3 @21:06:03.37 delay 3 seconds @21:06:06.44 AS.seek 0.604ml @21:06:11.78 delay 3 seconds @21:06:14.81 ATV.dial :bleach @21:06:15.42 AS.seek 0.589ml @21:06:18.74 delay 3 seconds @21:06:21.77 ATV.dial :ASV @21:06:22.82 ASV.dial :wasteR @21:06:23.44 AS.seek 0ml @21:08:22.21 delay 1:00 @21:09:22.25 AFV.select :carrierSolution @21:09:22.35 delay 2 seconds @21:09:24.37 AS.reconfigure ASconfig @21:09:24.43 AS.seek 1ml @21:10:22.19 delay 1 second @21:10:23.22 AFV.select :holdingCoil @21:10:23.32 delay 1 second @21:10:24.34 ATV.dial ASV @21:10:24.35 ASV.dial :wasteR @21:10:24.38 AS.reconfigure ASslow1 @21:10:24.45 AS.seek 0ml @21:13:45.79 delay 20 seconds @21:14:05.83 ATV.dial :air3 @21:14:07.35 AS.seek 0.59ml @21:16:06.35 delay 3 seconds @21:16:09.38 ATV.dial :ASV @21:16:10.80 ASV.dial :wasteR @21:16:10.89 AS.seek 0.09ml @21:17:51.72 delay 30 seconds @21:18:21.76 ATV.dial :extSrc @21:18:22.55 delay 3 seconds @21:18:25.57 ATV.dial :ElutionWater @21:18:26.69 AS.seek 0.12ml @21:18:33.04 ATV.dial :air3 @21:18:33.66 AS.seek 0.13ml @21:18:35.98 delay 2 seconds @21:18:38.01 ATV.dial :bleach @21:18:38.48 AS.seek 0.23ml @21:18:58.94 delay 3 seconds @21:19:01.97 ATV.dial :air3 @21:19:02.52 delay 3 seconds @21:19:05.60 AS.seek 0.255ml @21:19:10.88 delay 3 seconds @21:19:13.91 ATV.dial :bleach @21:19:14.52 AS.seek 0.24ml @21:19:17.82 delay 3 seconds @21:19:20.85 ATV.dial :ASV @21:19:21.90 ASV.dial :SPEdelivery @21:19:22.29 AFV.select :carrierSolution @21:19:22.41 delay 2 seconds @21:19:24.43 AS.reconfigure ASconfig @21:19:24.49 AS.seek 0.5ml @21:19:39.74 delay 1 second @21:19:40.77 AFV.select :holdingCoil @21:19:40.92 AS.reconfigure ASslow1 @21:19:40.98 AS.seek 0.17ml @21:20:47.64 SPE.hold 2:46:40,95C @21:20:47.71 delay 3:00 @21:23:47.74 AFV.select :carrierSolution @21:23:47.84 delay 2 seconds @21:23:49.86 AS.reconfigure ASconfig @21:23:49.92 AS.seek 1ml @21:24:37.95 delay 1 second @21:24:38.98 AFV.select :holdingCoil @21:24:39.08 delay 1 second @21:24:40.10 ASV.dial :wasteD @21:24:41.19 AS.seek 0ml @21:25:38.98 Priming Eluate @21:25:38.99 AFV.select :carrierSolution @21:25:39.12 delay 2 seconds @21:25:41.14 AS.seek 0.5ml @21:26:10.15 delay 1 second @21:26:11.18 AFV.select :holdingCoil @21:26:11.29 ATV.dial ASV @21:26:11.30 ASV.dial :SPErecovery @21:26:11.91 Swish #1 of 2 @21:26:12.00 AS.reconfigure ASslow0 @21:26:12.06 AS.seek 0.6ml @21:26:22.44 AS.seek 0.5ml @21:26:32.84 Swish #2 of 2 @21:26:32.93 AS.seek 0.6ml @21:26:43.33 AS.seek 0.5ml @21:26:54.16 delay 3 seconds @21:26:57.24 AS.reconfigure ASslow1 @21:26:57.29 AS.seek 0.684ml @21:27:34.57 delay 3 seconds @21:27:37.60 Eluate primed @21:27:37.61 ATV.dial :waste2 @21:27:38.35 AS.reconfigure ASnorm @21:27:38.41 AS.seek 0ml @21:28:13.23 SPE.coast @21:28:13.31 ASV.dial :SPErecovery @21:28:13.33 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118aug2621h.pcr @21:28:13.36 Building PCR Slug from ASV with MM2 mix, PP4 primer @21:28:13.38 Priming with carrier @21:28:13.41 ATV.dial ARV @21:28:14.38 ARV.dial :carrier @21:28:14.75 AS.seek 0.5ml @21:28:40.32 AFV.select :carrierSolution @21:28:40.45 delay 2 seconds @21:28:42.47 AS.reconfigure ASconfig @21:28:42.54 AS.seek 1ml @21:29:11.57 delay 1 second @21:29:12.60 AFV.select :holdingCoil @21:29:12.70 delay 1 second @21:29:13.72 ATV.dial :waste @21:29:15.24 AS.reconfigure ASnorm @21:29:15.30 AS.seek :empty @21:30:06.04 AFV.select :carrierSolution @21:30:06.17 delay 2 seconds @21:30:08.19 AS.reconfigure ASconfig @21:30:08.26 AS.seek 0.5ml @21:30:37.38 delay 1 second @21:30:38.40 AFV.select :holdingCoil @21:30:38.50 Building Slug @21:30:38.51 ATV.dial :ARV @21:30:40.14 delay 2 seconds @21:30:42.16 ARV.dial :air1 @21:30:42.58 delay 2 seconds @21:30:44.65 AS.reconfigure ASslow2 @21:30:44.71 AS.seek 0.506ml @21:30:50.99 delay 2 seconds @21:30:53.02 ARV.dial :MM2 @21:30:54.53 delay 2 seconds @21:30:56.55 Adding MM2 @21:30:56.64 AS.seek 0.524ml @21:31:14.85 delay 25 seconds @21:31:39.88 ARV.dial :PP4 @21:31:40.28 Adding PP4 @21:31:40.29 delay 2 seconds @21:31:42.37 AS.seek 0.53ml @21:31:48.63 delay 10 seconds @21:31:58.67 ARV.dial :air3 @21:31:59.07 adding ARV air @21:31:59.08 delay 2 seconds @21:32:01.16 AS.seek 0.536ml @21:32:07.37 delay 5 seconds @21:32:12.40 ARV.dial :carrier @21:32:13.69 delay 7 seconds @21:32:20.77 AS.seek 0.594ml @21:33:19.62 delay 5 seconds @21:33:24.65 ATV.dial :ASV @21:33:25.07 delay 2 seconds @21:33:27.14 AS.seek 0.6ml @21:33:33.37 delay 4 seconds @21:33:37.40 ATV.dial ARV @21:33:37.96 delay 2 seconds @21:33:39.98 ARV.dial :carrier @21:33:39.99 delay 2 seconds @21:33:42.07 AS.seek 0.65ml @21:34:32.73 delay 4 seconds @21:34:36.76 ATV.dial PCR @21:34:37.35 ASV.dial :waste1 @21:34:38.13 ARV.dial :air1 @21:34:38.54 Positioning Slug by 0.324ml @21:34:38.63 AS.seek 0.326ml @21:40:04.57 delay 5 seconds @21:40:09.60 ATV.dial ARV @21:40:10.35 ASV.dial :PCRlock @21:40:10.72 delay 5 seconds @21:40:15.74 Running Tt1 Profile60C300 thermocycling @21:40:15.75 PCR.program Profile60C300 @21:40:15.90 Profile60C300 should complete in < 1:45:21 @21:40:15.91 PCR.run 2:16:41.25 @23:01:09.56 PCR.off @23:01:11.05 PCR for Tt1 completed -- closed /var/log/esp/Tt118aug2621h.pcr @23:01:11.06 Full MFB Decontamination @23:01:11.09 Decon extraction column and decon AIV @23:01:11.12 SPE.hold 2:46:40,55C @23:01:11.17 ASV.dial :SPEdelivery @23:01:11.84 delay 20 seconds @23:01:31.87 AFV.select :carrierSolution @23:01:31.97 delay 2 seconds @23:01:33.99 AS.reconfigure ASconfig @23:01:34.05 AS.seek 0.5ml @23:01:44.35 delay 1 second @23:01:45.38 AFV.select :holdingCoil @23:01:45.48 ATV.dial :air1 @23:01:47.46 AS.reconfigure ASslow1 @23:01:47.52 AS.seek 0.52ml @23:01:51.77 ATV.dial :ASV @23:01:53.60 ASV.dial :SPErecovery @23:01:54.81 AS.seek 0.62ml @23:02:15.19 ATV.dial :waste @23:02:17.27 AS.reconfigure ASnorm @23:02:17.33 AS.seek :empty @23:02:48.93 AFV.select :holdingCoil @23:02:48.95 ATV.dial :waste @23:02:48.98 AS.reconfigure ASconfig @23:02:49.05 AS.seek 0ml @23:02:49.35 AFV.select :carrierSolution @23:02:49.47 delay 2 seconds @23:02:51.49 AS.seek 0.5ml @23:03:20.51 delay 1 second @23:03:21.54 AFV.select :holdingCoil @23:03:21.64 ATV.dial :air3 @23:03:22.37 AS.reconfigure ASslow1 @23:03:22.43 AS.seek 0.52ml @23:03:26.72 ATV.dial :air3 @23:03:26.82 AS.seek 0.53ml @23:03:29.13 delay 2 seconds @23:03:31.16 ATV.dial :bleach @23:03:31.64 AS.seek 0.71ml @23:04:08.08 delay 3 seconds @23:04:11.11 ATV.dial :air3 @23:04:11.70 delay 3 seconds @23:04:14.77 AS.seek 0.735ml @23:04:20.13 delay 3 seconds @23:04:23.16 ATV.dial :bleach @23:04:23.81 AS.seek 0.72ml @23:04:27.13 delay 3 seconds @23:04:30.16 ATV.dial ASV @23:04:31.26 ASV.dial :SPErecovery @23:04:31.36 AS.seek 0.365ml @23:05:43.01 ATV.dial :waste @23:05:44.78 AS.reconfigure ASnorm @23:05:44.84 AS.seek :empty @23:06:03.62 ATV.dial :air3 @23:06:04.30 AS.reconfigure ASslow1 @23:06:04.37 AS.seek 0.009ml @23:06:06.72 delay 2 seconds @23:06:08.75 ATV.dial :bleach @23:06:09.23 AS.seek 0.579ml @23:08:04.12 delay 3 seconds @23:08:07.15 ATV.dial :air3 @23:08:07.74 delay 3 seconds @23:08:10.81 AS.seek 0.604ml @23:08:16.13 delay 3 seconds @23:08:19.16 ATV.dial :bleach @23:08:19.78 AS.seek 0.589ml @23:08:23.07 delay 3 seconds @23:08:26.10 ATV.dial :ASV @23:08:27.15 ASV.dial :wasteR @23:08:27.56 AS.seek 0ml @23:10:26.33 delay 1:00 @23:11:26.37 ATV.dial ASV @23:11:26.38 AFV.select :carrierSolution @23:11:26.51 delay 2 seconds @23:11:28.53 AS.reconfigure ASconfig @23:11:28.59 AS.seek 1ml @23:12:26.35 delay 1 second @23:12:27.38 AFV.select :holdingCoil @23:12:27.49 delay 1 second @23:12:28.51 ASV.dial :wasteR @23:12:28.52 AS.reconfigure ASslow1 @23:12:28.59 AS.seek 0ml @23:15:49.99 delay 10 seconds @23:16:00.03 ATV.dial :air3 @23:16:01.55 AS.seek 0.01ml @23:16:03.85 delay 2 seconds @23:16:05.88 ATV.dial :bleach @23:16:06.52 AS.seek 0.58ml @23:18:01.43 delay 3 seconds @23:18:04.46 ATV.dial :air3 @23:18:05.03 delay 3 seconds @23:18:08.10 AS.seek 0.605ml @23:18:13.41 delay 3 seconds @23:18:16.44 ATV.dial :bleach @23:18:17.08 AS.seek 0.59ml @23:18:20.38 delay 3 seconds @23:18:23.41 ATV.dial ASV @23:18:24.56 ASV.dial :SPEdelivery @23:18:24.94 AS.seek 0ml @23:20:23.87 delay 1:00 @23:21:23.91 AFV.select :carrierSolution @23:21:24.01 delay 2 seconds @23:21:26.03 AS.reconfigure ASconfig @23:21:26.09 AS.seek 1ml @23:22:24.34 delay 1 second @23:22:25.37 AFV.select :holdingCoil @23:22:25.47 delay 1 second @23:22:26.49 ATV.dial ASV @23:22:26.50 ASV.dial :SPEdelivery @23:22:26.53 AS.reconfigure ASslow1 @23:22:26.60 AS.seek 0ml @23:25:47.99 delay 10 seconds @23:25:58.03 ATV.dial :air2 @23:25:58.86 AS.reconfigure ASslow0 @23:25:58.92 AS.seek 0.8ml @23:27:19.70 delay 3 seconds @23:27:22.73 ATV.dial :ASV @23:27:23.71 ASV.dial :SPEdelivery @23:27:23.74 AS.reconfigure ASslow1 @23:27:23.81 AS.seek 0.03ml @23:29:58.98 delay 20 seconds @23:30:19.02 ATV.dial :waste,via: :air3 @23:30:20.76 AS.reconfigure ASconfig @23:30:20.82 AS.seek :empty @23:30:22.90 SPE.coast @23:30:22.99 Removing Bleach from Mixing Coil @23:30:23.00 AFV.select :carrierSolution @23:30:23.12 delay 2 seconds @23:30:25.14 AS.seek 0.5ml @23:30:54.24 delay 1 second @23:30:55.27 AFV.select :holdingCoil @23:30:55.37 ATV.dial :ASV @23:30:57.07 ASV.dial :SPErecovery @23:30:57.73 AS.reconfigure ASslow1 @23:30:57.80 AS.seek 0.73ml @23:31:44.37 ASV.dial :wasteD @23:31:45.05 AS.reconfigure ASnorm @23:31:45.11 AS.seek 0ml @23:32:22.18 AFV.select :carrierSolution @23:32:22.30 delay 2 seconds @23:32:24.32 AS.reconfigure ASconfig @23:32:24.38 AS.seek 0.5ml @23:32:53.43 delay 1 second @23:32:54.46 AFV.select :holdingCoil @23:32:54.56 ATV.dial ASV @23:32:54.57 ASV.dial :SPErecovery @23:32:55.23 AS.reconfigure ASslow0 @23:32:55.30 AS.seek 0.3ml @23:33:15.71 ATV.dial :air2 @23:33:16.76 AS.reconfigure ASslow1 @23:33:16.83 AS.seek 0.32ml @23:33:21.20 ATV.dial ASV @23:33:22.24 AS.reconfigure ASslow0 @23:33:22.31 AS.seek 0.57ml @23:33:47.78 ASV.dial :wasteD @23:33:48.38 AS.reconfigure ASnorm @23:33:48.44 AS.seek 0ml @23:34:17.53 AFV.select :carrierSolution @23:34:17.65 delay 2 seconds @23:34:19.67 AS.reconfigure ASconfig @23:34:19.73 AS.seek 0.5ml @23:34:48.79 delay 1 second @23:34:49.82 AFV.select :holdingCoil @23:34:49.92 ATV.dial ASV @23:34:49.93 ASV.dial :SPErecovery @23:34:50.59 AS.reconfigure ASslow0 @23:34:50.65 AS.seek 0.3ml @23:35:11.07 ATV.dial :air2 @23:35:12.12 AS.reconfigure ASslow1 @23:35:12.19 AS.seek 0.32ml @23:35:16.55 ATV.dial ASV @23:35:17.60 AS.reconfigure ASslow0 @23:35:17.66 AS.seek 0.57ml @23:35:43.12 ASV.dial :wasteD @23:35:43.72 AS.reconfigure ASnorm @23:35:43.78 AS.seek 0ml @23:36:12.86 ATV.dial :air2 @23:36:13.85 AS.reconfigure ASslow0 @23:36:13.91 AS.seek 0.3ml @23:36:44.40 ATV.dial :ASV @23:36:45.39 ASV.dial :wasteD @23:36:45.41 AS.reconfigure ASconfig @23:36:45.49 AS.seek 0.05ml @23:37:00.18 ATV.dial :waste @23:37:02.05 AS.seek 0ml @23:37:05.27 ASV.dial :SPEdelivery @23:37:06.27 deconLysateINT @23:37:06.28 ATV.dial :air3 @23:37:06.98 AS.reconfigure ASslow1 @23:37:07.05 AS.seek 0.01ml @23:37:09.39 delay 2 seconds @23:37:11.42 ATV.dial :bleach @23:37:12.38 AS.seek 0.96ml @23:40:23.68 delay 3 seconds @23:40:26.71 ATV.dial :air3 @23:40:27.27 delay 3 seconds @23:40:30.34 AS.seek 0.985ml @23:40:35.69 delay 3 seconds @23:40:38.72 ATV.dial :bleach @23:40:39.35 AS.seek 0.97ml @23:40:42.67 delay 3 seconds @23:40:45.70 ATV.dial AIV @23:40:46.94 AIV.dial :coilToMFB @23:40:46.95 AWV.select :waste @23:40:46.96 AFV.select :carrierSolution @23:40:47.10 delay 2 seconds @23:40:49.12 AS.reconfigure ASconfig @23:40:49.19 AS.seek 1ml @23:40:51.22 delay 1 second @23:40:52.25 AFV.select :holdingCoil @23:40:52.35 delay 1 second @23:40:53.37 AS.reconfigure ASslow0 @23:40:53.43 AS.seek :empty @23:42:34.42 AFV.select :carrierSolution @23:42:34.54 delay 2 seconds @23:42:36.56 AS.reconfigure ASconfig @23:42:36.63 AS.seek 1ml @23:43:34.45 delay 1 second @23:43:35.48 AFV.select :holdingCoil @23:43:35.58 delay 1 second @23:43:36.60 AS.reconfigure ASslow0 @23:43:36.66 AS.seek :empty @23:45:17.65 AFV.select :carrierSolution @23:45:17.78 delay 2 seconds @23:45:19.80 AS.reconfigure ASconfig @23:45:19.87 AS.seek 1ml @23:46:17.70 delay 1 second @23:46:18.73 AFV.select :holdingCoil @23:46:18.83 delay 1 second @23:46:19.85 AS.reconfigure ASslow0 @23:46:19.91 AS.seek :empty @23:48:00.87 ATV.dial :air2 @23:48:01.54 AS.reconfigure ASslow1 @23:48:01.61 AS.seek 0.009ml @23:48:03.95 AS.reconfigure ASslow0 @23:48:04.01 AS.seek 1ml @23:49:43.95 ATV.dial AIV @23:49:44.73 AS.seek 0.02ml @23:51:23.64 ATV.dial :waste @23:51:25.38 AS.seek 0ml @23:51:27.67 AWV.select :air @23:51:27.83 Cleaning PCR @23:51:27.84 AFV.select :carrierSolution @23:51:27.97 delay 2 seconds @23:51:29.99 AS.reconfigure ASconfig @23:51:30.06 AS.seek 0.5ml @23:51:59.14 delay 1 second @23:52:00.17 AFV.select :holdingCoil @23:52:00.27 ASV.dial :waste1 @23:52:00.64 ATV.dial :air3 @23:52:01.32 AS.reconfigure ASslow1 @23:52:01.38 AS.seek 0.51ml @23:52:03.66 delay 2 seconds @23:52:05.69 ATV.dial :bleach @23:52:06.14 AS.seek 0.81ml @23:53:06.72 delay 3 seconds @23:53:09.75 ATV.dial :air3 @23:53:10.32 delay 3 seconds @23:53:13.39 AS.seek 0.835ml @23:53:18.71 delay 3 seconds @23:53:21.74 ATV.dial :bleach @23:53:22.36 AS.seek 0.82ml @23:53:25.62 delay 3 seconds @23:53:28.65 ARV.dial :waste @23:53:29.28 delay 3 seconds @23:53:32.30 ATV.dial :ARV @23:53:33.18 AS.reconfigure ASconfig @23:53:33.24 AS.seek 0.47ml @23:53:53.66 AFV.select :carrierSolution @23:53:53.78 delay 2 seconds @23:53:55.80 AS.seek 1ml @23:54:26.58 delay 1 second @23:54:27.61 AFV.select :holdingCoil @23:54:27.71 delay 1 second @23:54:28.73 AS.reconfigure ASfast @23:54:28.79 AS.seek 0ml @23:54:56.14 AFV.select :carrierSolution @23:54:56.26 delay 2 seconds @23:54:58.28 AS.reconfigure ASconfig @23:54:58.34 AS.seek 1ml @23:55:56.09 delay 1 second @23:55:57.12 AFV.select :holdingCoil @23:55:57.22 delay 1 second @23:55:58.24 AS.reconfigure ASfast @23:55:58.30 AS.seek 0ml @23:56:25.66 AFV.select :carrierSolution @23:56:25.78 delay 2 seconds @23:56:27.80 AS.reconfigure ASconfig @23:56:27.86 AS.seek 1ml @23:57:25.64 delay 1 second @23:57:26.67 AFV.select :holdingCoil @23:57:27.29 delay 1 second @23:57:28.31 AS.reconfigure ASfast @23:57:28.38 AS.seek 0ml @23:57:55.72 ARV.dial between carrier and air1 @23:57:56.23 ATV.dial :air3 @23:57:57.51 AS.reconfigure ASslow1 @23:57:57.57 AS.seek 0.01ml @23:57:59.98 delay 2 seconds @23:58:02.01 ATV.dial :bleach @23:58:02.63 AS.seek 0.81ml @00:00:43.78CEST27-Aug-18 delay 3 seconds @00:00:46.81 ATV.dial :air3 @00:00:47.37 delay 3 seconds @00:00:50.44 AS.seek 0.835ml @00:00:55.75 delay 3 seconds @00:00:58.78 ATV.dial :bleach @00:00:59.40 AS.seek 0.82ml @00:01:02.66 delay 3 seconds @00:01:05.69 ATV.dial PCR @00:01:06.09 AS.reconfigure ASconfig @00:01:06.15 AS.seek :empty @00:01:53.61 PCR.program :bleachProfile @00:01:53.67 PCR program should complete in < 2:45 @00:01:53.69 PCR.run 10:00 @00:05:01.57 PCR.off @00:05:01.61 AFV.select :carrierSolution @00:05:01.72 delay 2 seconds @00:05:03.74 AS.seek 1ml @00:06:01.58 delay 1 second @00:06:02.61 AFV.select :holdingCoil @00:06:02.71 delay 1 second @00:06:03.73 AS.reconfigure ASfast @00:06:03.80 AS.seek 0ml @00:06:31.16 delay 3 seconds @00:06:34.19 AFV.select :carrierSolution @00:06:34.29 delay 2 seconds @00:06:36.31 AS.reconfigure ASconfig @00:06:36.38 AS.seek 1ml @00:07:34.14 delay 1 second @00:07:35.17 AFV.select :holdingCoil @00:07:35.27 delay 1 second @00:07:36.29 AS.reconfigure ASfast @00:07:36.35 AS.seek 0ml @00:08:03.71 delay 3 seconds @00:08:06.74 AFV.select :carrierSolution @00:08:06.84 delay 2 seconds @00:08:08.86 AS.reconfigure ASconfig @00:08:08.92 AS.seek 1ml @00:09:06.66 delay 1 second @00:09:07.69 AFV.select :holdingCoil @00:09:07.79 delay 1 second @00:09:08.81 AS.reconfigure ASfast @00:09:08.87 AS.seek 0ml @00:09:36.22 delay 3 seconds @00:09:39.25 AFV.select :carrierSolution @00:09:39.35 delay 2 seconds @00:09:41.37 AS.reconfigure ASconfig @00:09:41.45 AS.seek 1ml @00:10:39.19 delay 1 second @00:10:40.22 AFV.select :holdingCoil @00:10:40.32 delay 1 second @00:10:41.34 AS.reconfigure ASfast @00:10:41.40 AS.seek 0ml @00:11:08.75 delay 3 seconds @00:11:11.78 AFV.select :carrierSolution @00:11:11.88 delay 2 seconds @00:11:13.90 AS.reconfigure ASconfig @00:11:13.96 AS.seek 1ml @00:12:11.74 delay 1 second @00:12:12.77 AFV.select :holdingCoil @00:12:12.87 delay 1 second @00:12:13.89 AS.reconfigure ASnorm @00:12:13.95 AS.seek 0ml @00:13:04.66 AFV.select :carrierSolution @00:13:04.77 delay 2 seconds @00:13:06.79 AS.reconfigure ASconfig @00:13:06.85 AS.seek 1ml @00:14:04.60 delay 1 second @00:14:05.63 AFV.select :holdingCoil @00:14:05.73 delay 1 second @00:14:06.75 ATV.dial :waste @00:14:07.97 AS.reconfigure ASnorm @00:14:08.03 AS.seek 0.5ml @00:14:33.60 ATV.dial :waste2 @00:14:34.89 AS.seek 0ml @00:15:00.44
Asleep after completing 18tun assays @00:15:00.46 GoodNews.email "{1 => 2, 2 => 0}\nCan@00:12:57, 16.8C, 41% humidity, 14.6psia, 12.065V, 0.418A, 0.418A avg, 5.04W",Subject: "Asleep after completing 18tun assays" @00:15:00.49
Mission runtime was 4:32:01.682239 @00:15:10.37 Session Ended @23:50:14.21CEST28-Aug-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @23:50:15.86 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @23:50:16.11 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @23:50:20.45 Polling Can every 10:00 @23:50:22.20 SPE.coast @23:50:22.25 SPE.configure SPEconfig @23:50:22.33 AS.reconfigure ASconfig @23:50:22.37 AS.hold @23:50:22.41 AS.configure ASconfig @23:50:22.55 SS.reconfigure SSconfig @23:50:22.61 SS.hold @23:50:22.71 SS.configure SSconfig @23:50:22.89 CC.reconfigure CCconfig @23:50:22.95 CC.hold @23:50:23.05 CC.configure CCconfig @23:50:23.21 CS.reconfigure CSconfig @23:50:23.27 CS.hold @23:50:23.33 CS.configure CSconfig @23:50:23.74 PC.reconfigure PCconfig @23:50:23.78 PC.hold @23:50:23.82 PC.configure PCconfig @23:50:23.90 PS.reconfigure PSconfig @23:50:23.94 PS.hold @23:50:24.02 PS.configure PSconfig @23:50:24.12 Forearm.reconfigure LooseForearm @23:50:24.16 Forearm.hold @23:50:24.20 Forearm.configure LooseForearm @23:50:24.28 Elbow.reconfigure LooseElbow @23:50:24.32 Elbow.hold @23:50:24.38 Elbow.configure LooseElbow @23:50:24.47 SC.reconfigure SCloose @23:50:24.51 SC.hold @23:50:24.55 SC.configure SCloose @23:50:24.63 SE.reconfigure SEconfig @23:50:24.67 SE.hold @23:50:24.73 SE.configure SEconfig @23:50:24.77 CH.coast @23:50:24.81 CH.configure CHconfig @23:50:24.84 PH.coast @23:50:24.89 PH.configure PHconfig @23:50:24.92 PCR.off @23:50:24.95 PCR.pidtemp= 50,0.1,0,300,25,25 @23:50:24.98 PCR.caltemp= 2.7073,0.0373,0 @23:50:25.01 All dwarf controllers running firmware version 3.75 @23:50:25.12 Cleanly exited at 23:49:41CEST28-Aug-18 @23:50:25.15 Loading /home/esp/esp2/mission/18tun.rb mission @23:50:25.20 Preparing to sleep until +0:00:15 @23:50:25.22 GoodNews.email "Starting 18tun w/pucks in tube 2\n{}\nCan@23:50:20, 17.2C, 38% humidity, 14.7psia, 12.011V, 0.651A, 0.651A avg, 7.82W",Subject: "18tun begun" @23:50:25.29 AS.coast @23:50:25.32
Asleep til +0:00:15 @23:50:25.36 SS.coast @23:50:25.37
Continuing at 23:50 @23:50:25.46 CC.coast @23:50:25.50 CS.coast @23:50:25.54 PC.coast @23:50:25.57 PS.coast @23:50:25.60 Forearm.coast @23:50:25.63 Elbow.coast @23:50:25.66 SC.coast @23:50:25.70 SE.coast @23:50:26.12 Gate.power :core,:OFF @23:50:26.16 Gate.power :raw,:OFF @23:50:26.20 Shutdown @23:50:26.38 Session Begun @23:50:32.18 GoodNews.email "Then, in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:106\n{}\nCan@23:50:25, 17.2C, 38% humidity, 14.7psia, 12.016V, 0.645A, 0.645A avg, 7.75W",Subject: "Asleep until 23:50" @23:50:40.18
Powering core bus @23:50:45.03 Gate.power :raw,:ON @23:50:45.62 SPE.coast @23:50:45.69 SPE.configure SPEconfig @23:50:45.79 AS.configure ASconfig @23:50:45.90 SS.configure SSconfig @23:50:46.00 CC.configure CCconfig @23:50:46.10 CS.configure CSconfig @23:50:46.20 PC.configure PCconfig @23:50:46.30 PS.configure PSconfig @23:50:46.43 Forearm.configure LooseForearm @23:50:46.54 Elbow.configure LooseElbow @23:50:46.66 SC.configure SCloose @23:50:46.76 SE.configure SEconfig @23:50:46.82 CH.coast @23:50:46.88 CH.configure CHconfig @23:50:46.92 PH.coast @23:50:46.99 PH.configure PHconfig @23:50:47.04 PCR.off @23:50:47.08 PCR.pidtemp= 50,0.1,0,300,25,25 @23:50:47.11 PCR.caltemp= 2.7073,0.0373,0 @23:50:47.15 All dwarf controllers running firmware version 3.75 @23:50:47.27 Valve.reset @23:50:47.44 PCR.off @23:50:47.47 PCR.pidtemp= 50,0.1,0,300,25,25 @23:50:47.50 PCR.caltemp= 2.7073,0.0373,0 @23:50:47.66 Intake.select :close @23:50:47.69 Exhaust.select :close @23:50:47.75 CTV.dial :air @23:50:47.92 CSV.dial :waste1 @23:50:47.94 PSV.dial :air @23:50:48.05
Forearm.reconfigure LooseForearm @23:50:48.47 Forearm.home.seek :retract @23:50:48.55 SSV.dial :spit,avoiding: :exhaust @23:50:48.58 PS.reconfigure PSconfig @23:50:48.69 PS.home.seek 0ml @23:50:48.81 CS.reconfigure CSfast @23:50:48.84 SS.reconfigure SSconfig @23:50:48.93 CS.home.seek 0ml @23:50:48.96 SS.home.seek 0ml @23:50:49.22 Hand.close @23:50:49.27
Elbow.jog 1500 @23:50:50.00 Elbow.reconfigure LooseElbow @23:50:50.06 Elbow.home.seek :clear @23:50:53.39 SE.reconfigure SEconfig @23:50:53.45 SE.home.seek :down @23:50:54.06 SC.reconfigure SCsloppy @23:50:54.12 SC.home.seek :initial @23:50:58.02 PS.coast @23:50:58.24
Forearm.seek :retract @23:50:58.32 Elbow.seek :hammer @23:50:59.53 Forearm.reconfigure TightForearm @23:50:59.60 Forearm.seek 5 + 350 counts @23:51:00.69 Forearm.seek :retract @23:51:01.86 CC.reconfigure CCconfig @23:51:01.88 PC.reconfigure PCconfig @23:51:01.99
CC.home.seek :open @23:51:02.01 PC.home.seek :open @23:51:05.32
Phase [coreNEG] @23:51:05.34 GoodNews.email "At 23:51:05 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:106\n{}\nCan@23:51:05, 17.4C, 38% humidity, 14.7psia, 11.987V, 0.764A, 0.764A avg, 9.16W",Subject: "Started 18tun phase coreNEG" @23:51:05.37CEST28-Aug-18
==================> STARTED coreNEG (phase #1) <=== @23:51:05.40 Starting initialPurge @23:51:05.43 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @23:51:05.51 Hand.open @23:51:06.35 Elbow.reconfigure TightElbow @23:51:06.41 Elbow.seek FlushPuck::Garage @23:51:08.48 Elbow.reconfigure LooseElbow @23:51:08.59 Forearm.reconfigure LooseForearm @23:51:08.65 Forearm.seek :garage @23:51:09.67 Hand.close @23:51:10.58 Forearm.seek :retract @23:51:11.61 Elbow.reconfigure TightElbow @23:51:11.67 Elbow.seek CC @23:51:14.68 Elbow.reconfigure LooseElbow @23:51:14.79 Forearm.reconfigure TightForearm @23:51:14.86 Forearm.seek CC @23:51:16.05 CC.seek :closed @23:51:20.40 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.34 @23:51:20.48 Hand.open @23:51:21.27 Forearm.reconfigure LooseForearm @23:51:21.33 Forearm.seek :retract @23:51:22.31 Flushing the Collection Valve::Manifold @23:51:22.44 CSV.dial CSR @23:51:24.00 Pulling 5ml of CSR.flush @23:51:24.01 CSR.select :flush @23:51:24.15 CS.reconfigure CSconfig @23:51:24.21 CS.seek 5ml @23:51:38.12 delay 5 seconds @23:51:43.14 CSR.select :air @23:51:43.32 CS.reconfigure CSfast @23:51:43.38 CS.seek 7.5ml @23:51:46.80 -> exit @23:51:46.82 Session Ended @23:51:50.84
CSV.dial :waste @23:51:52.31 CS.reconfigure CSconfig @23:51:52.35 CS.seek 0ml @23:52:12.47 Purging external Intake @23:52:12.58 CSV.dial :flush @23:52:13.06 CS.reconfigure CSfast @23:52:13.11 CS.seek 10ml @23:52:39.94 delay 5 seconds @23:52:44.96 CSV.dial CBV @23:52:45.00
CBV.dial CSV @23:52:45.88 CTV.dial :intake @23:52:46.75 Intake.select :open @23:52:46.86 CS.seek 0ml @23:53:14.22 Intake.select :close @23:53:14.32 CTV.dial :air @23:53:15.19 Flushing upper manifold @23:53:15.30 CSV.dial :flush @23:53:16.13 CS.seek 5ml @23:53:29.90 delay 5 seconds @23:53:34.92 CSV.dial :air @23:53:35.50 CS.seek 7ml @23:53:41.62 CSV.dial CBV @23:53:41.64
CBV.dial CSV @23:53:42.83 CTV.dial :air @23:53:42.85 CS.seek 0ml @23:54:02.03 delay 7 seconds @23:54:09.06 CS.seek 3ml @23:54:17.62 CSV.dial :waste @23:54:18.13 CS.seek 0ml @23:54:26.91 CSV.dial :flush @23:54:27.79 CS.seek 3ml @23:54:36.53 delay 5 seconds @23:54:41.55 CSV.dial :air @23:54:42.11 CS.seek 5ml @23:54:48.16 CSV.dial :air @23:54:48.18 CS.seek 0ml @23:55:01.75 delay 7 seconds @23:55:08.78 CS.seek 3ml @23:55:17.44 CSV.dial :waste @23:55:18.28 CS.seek 0ml @23:55:26.91 Garaging FlushPuck in Collection Clamp @23:55:26.98 Elbow.reconfigure TightElbow @23:55:27.02 Elbow.seek CC @23:55:27.26 Elbow.reconfigure LooseElbow @23:55:27.35 Forearm.seek CC @23:55:28.33 Hand.close @23:55:29.08 CC.seek :open @23:55:33.45 Forearm.seek :retract @23:55:34.46 Elbow.reconfigure TightElbow @23:55:34.50 Elbow.seek :garage @23:55:37.55 Elbow.reconfigure LooseElbow @23:55:37.64 Forearm.reconfigure TightForearm @23:55:37.68 Forearm.seek :garage @23:55:38.83 Hand.open @23:55:39.64 Forearm.reconfigure LooseForearm @23:55:39.68 Forearm.seek :retract @23:55:40.79 Starting mfb @23:55:40.81 pcrBAC @23:55:40.83 BACfans.email "Started mfb in tube 2 at 23:55:40CEST28-Aug-18\n{}\nCan@23:51:05, 17.4C, 38% humidity, 14.7psia, 11.987V, 0.764A, 0.764A avg, 9.16W",Subject: "pcrBAC" @23:55:40.89
Loading sh1 puck from tube 2 into Collection Clamp @23:55:40.98 Readying the MFB @23:55:41.04 Homing MFB Syringe @23:55:41.05 AFV.select :holdingCoil @23:55:41.27 ATV.dial :air2 @23:55:41.35
SC.reconfigure SCloose @23:55:41.58 Elbow.reconfigure TightElbow @23:55:41.62 Elbow.seek 2 @23:55:41.85 AS.reconfigure ASconfig @23:55:41.90 AS.home.seek :home @23:55:43.67
Forearm.reconfigure TightForearm @23:55:43.71 Forearm.seek 2 + 700 counts @23:55:44.79 SE.reconfigure SEpick @23:55:44.87 SE.seek beamBreak @23:55:47.44 AFV.select :carrierSolution @23:55:47.54 delay 2 seconds @23:55:48.05
SE.configure SEconfig @23:55:48.20 Elbow.seek 2 @23:55:48.39 Forearm.seek 2 + 300 counts @23:55:48.65 Forearm.reconfigure LooseForearm @23:55:48.69 Hand.close @23:55:49.47 SE.seek :down @23:55:49.67 AS.seek 1ml @23:55:49.75
Forearm.seek :retract @23:55:50.75 Elbow.seek CC @23:55:52.33 SE.coast @23:55:52.81
Elbow.reconfigure LooseElbow @23:55:52.97 Forearm.reconfigure TightForearm @23:55:53.02 Forearm.seek CC @23:55:54.24 CC.seek :closed @23:55:58.49 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.47 @23:55:58.50 Hand.open @23:55:59.27 Forearm.reconfigure LooseForearm @23:55:59.31 Forearm.seek :retract @23:56:00.30 SC.coast @23:56:00.33 skipping prime and sample collection @23:56:00.35 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @23:56:00.42 CSV.dial :waste @23:56:00.47 CS.seek 0ml @23:56:00.59
Calibrating pressure and temperature sensors @23:56:00.61 CTV.dial :air @23:56:00.62 SSV.dial :spit,avoiding: :exhaust @23:56:00.64 SS.reconfigure SSfast @23:56:00.68 SS.seek 5ml @23:56:00.81 CSV.dial :air @23:56:01.65 CS.seek 10ml @23:56:03.80 SSV.dial CBV @23:56:03.83
CBV.dial SSV @23:56:05.37 delay 3.8 seconds @23:56:09.29 SSV.dial :spit @23:56:10.93 SS.seek 0ml @23:56:13.69 SSV.dial CBV @23:56:15.17 SSV.dial CBV,avoiding: :exhaust @23:56:15.20
CBV.dial SSV @23:56:15.21 CTV.dial :air @23:56:15.22 delay 15 seconds @23:56:30.24 SSV.dial :spit,avoiding: :exhaust @23:56:31.73 CTV.dial CSV,avoiding: :intake @23:56:31.77
SS.reconfigure SSexhale @23:56:31.86 SS.seek 0ml @23:56:31.92 SSV.dial CBV,avoiding: :exhaust @23:56:33.48 CBV.dial between SSV and waste2 @23:56:33.89 CTV.dial CSV,avoiding: :air @23:56:33.92
CSV.dial CTV @23:56:34.42 CS.reconfigure CSpressurize @23:56:34.46 CS.seek 0ml @23:56:42.59 delay 1 second @23:56:43.61 AFV.select :holdingCoil @23:56:43.71 delay 1 second @23:56:44.73 ATV.dial :waste @23:56:45.78 AS.reconfigure ASnorm @23:56:45.83 AS.home.seek :empty @23:57:00.56
CSV.dial :air @23:57:01.06 CS.reconfigure CSfast @23:57:01.11 CS.seek 10ml @23:57:27.56 CSV.dial between air and CTV @23:57:27.92 CS.seek 3.419ml @23:57:38.37 ATV.dial :waste @23:57:38.38 ARV.dial between carrier and air1 @23:57:38.45 ASV.dial :waste1 @23:57:45.60 CTV.dial CSV,avoiding: :air @23:57:45.62
CSV.dial CTV @23:57:45.95 CS.reconfigure CSpressurize @23:57:45.99 CS.seek 0ml @23:57:55.47 CSV.dial :air @23:57:55.97 CS.reconfigure CSfast @23:57:56.02 CS.seek 10ml @23:58:22.30 CSV.dial between air and CTV @23:58:22.67 CS.seek 2.165ml @23:58:43.99 CTV.dial CSV,avoiding: :air @23:58:44.01
CSV.dial CTV @23:58:44.36 CS.reconfigure CSpressurize @23:58:44.40 CS.seek 0ml @23:58:46.42 BubblePoint: Intake @70.1psia, Outlet @14.7psia CBV.dial SSV @23:58:46.79 SS.reconfigure SSconfig @23:58:46.83 SS.seek 10ml @23:58:52.97 delay 15 seconds @23:59:08.09 Evacuated: Intake @28.6psia, Outlet @28.9psia @23:59:08.10 SSV.dial :spit,avoiding: :exhaust @23:59:09.60 SS.reconfigure SSexhale @23:59:09.64 SS.seek 0ml @23:59:14.61 SS.reconfigure SSfast @23:59:14.65 SS.seek 5ml @23:59:17.42 SSV.dial CBV,avoiding: :exhaust @23:59:17.45
CBV.dial SSV @23:59:18.94 CTV.dial :air,avoiding: :intake @23:59:20.46 CSV.dial CTV @23:59:20.50
SS.seek 10ml @23:59:21.49 CSV.dial :air @23:59:22.00 CS.reconfigure CSfast @23:59:22.05 CS.seek 0ml @23:59:23.47
SSV.dial :spit,avoiding: :exhaust @23:59:24.96 SS.reconfigure SSexhale @23:59:25.00 SS.seek 0ml @23:59:27.19 CS.coast @23:59:30.02
SS.reconfigure SSfast @23:59:30.06 SS.seek 5ml @23:59:33.09 SSV.dial CBV,avoiding: :exhaust @23:59:33.12
CBV.dial SSV @23:59:35.63 SSV.dial :spit,avoiding: :exhaust @23:59:37.14 SS.reconfigure SSconfig @23:59:37.19 SS.seek 0ml @23:59:40.19 SS.coast @23:59:40.22 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @23:59:40.23 Intake.select :close @23:59:40.30 CSV.dial :waste @23:59:40.41 SSV.dial CBV,avoiding: :exhaust @23:59:40.43
CBV.dial SSV @23:59:41.17 CS.seek 0ml @23:59:41.41 CSV.dial :air @23:59:41.92
CTV.dial :air @23:59:41.93 delay 15 seconds @23:59:42.25 CS.seek 10ml @23:59:56.95
SSV.dial :spit,avoiding: :exhaust @23:59:58.45 CTV.dial CSV,avoiding: :intake @23:59:58.49
SS.reconfigure SSexhale @23:59:58.58 SS.seek 0ml @23:59:58.63 SSV.dial CBV,avoiding: :exhaust @00:00:08.72CEST29-Aug-18 CBV.dial between SSV and waste2 @00:00:09.14 CTV.dial CSV,avoiding: :air @00:00:09.16
CSV.dial CTV @00:00:09.65 CS.reconfigure CSpressurize @00:00:09.69 CS.seek 0ml @00:00:35.79 CSV.dial :air @00:00:36.41 CS.reconfigure CSfast @00:00:36.45 CS.seek 10ml @00:01:02.74 CSV.dial between air and CTV @00:01:03.09 CS.seek 3.408ml @00:01:20.69 CTV.dial CSV,avoiding: :air @00:01:20.71
CSV.dial CTV @00:01:21.05 CS.reconfigure CSpressurize @00:01:21.09 CS.seek 0ml @00:01:30.54 CSV.dial :air @00:01:31.05 CS.reconfigure CSfast @00:01:31.10 CS.seek 10ml @00:01:57.35 CSV.dial between air and CTV @00:01:57.70 CS.seek 2.151ml @00:02:18.69 CTV.dial CSV,avoiding: :air @00:02:19.07
CSV.dial CTV @00:02:19.47 CS.reconfigure CSpressurize @00:02:19.51 CS.seek 0ml @00:02:21.45 BubblePoint: Intake @70.3psia, Outlet @15.2psia @00:02:21.46 CBV.dial SSV @00:02:21.83 SS.reconfigure SSconfig @00:02:21.87 SS.seek 10ml @00:02:28.00 delay 15 seconds @00:02:43.12 Evacuated: Intake @28.7psia, Outlet @29.0psia @00:02:43.13 SSV.dial :spit,avoiding: :exhaust @00:02:44.64 SS.reconfigure SSexhale @00:02:44.69 SS.seek 0ml @00:02:49.69 SS.reconfigure SSfast @00:02:49.73 SS.seek 5ml @00:02:52.72 SSV.dial CBV,avoiding: :exhaust @00:02:52.75
CBV.dial SSV @00:02:54.26 CTV.dial :air,avoiding: :intake @00:02:55.77 CSV.dial CTV @00:02:55.81
SS.seek 10ml @00:02:56.81 CSV.dial :air @00:02:57.33 CS.reconfigure CSfast @00:02:57.38 CS.seek 0ml @00:02:58.48
SSV.dial :spit,avoiding: :exhaust @00:02:59.98 SS.reconfigure SSexhale @00:03:00.02 SS.seek 0ml @00:03:02.44 CS.coast @00:03:05.22
SS.reconfigure SSfast @00:03:05.26 SS.seek 5ml @00:03:08.26 SSV.dial CBV,avoiding: :exhaust @00:03:08.29
CBV.dial SSV @00:03:10.80 SSV.dial :spit,avoiding: :exhaust @00:03:12.31 SS.reconfigure SSconfig @00:03:12.36 SS.seek 0ml @00:03:15.56 SS.coast @00:03:15.59 Lysing: Adding 1.5ml lysis @00:03:15.60 CBV.dial between CSV and waste1,avoiding: :DBV @00:03:17.31 CTV.dial CSV @00:03:18.82 CSV.dial CSR @00:03:19.70 Pulling 1ml of CSR.lysis @00:03:19.71 CSR.select :lysis @00:03:19.82 CS.reconfigure CSconfig @00:03:19.86 CS.seek 1ml @00:03:23.47 delay 5 seconds @00:03:28.49 CSR.select :air @00:03:28.65 CS.seek 6ml @00:03:42.48 delay 2 seconds @00:03:44.50 CSV.dial :waste1 @00:03:45.98 CS.reconfigure CSfast @00:03:46.02 CS.seek :empty @00:04:02.29 CSV.dial CSR @00:04:03.81 Pulling 1.5ml of CSR.lysis @00:04:03.82 CSR.select :lysis @00:04:03.93 CS.reconfigure CSconfig @00:04:03.97 CS.seek 1.5ml @00:04:08.85 delay 5 seconds @00:04:13.87 CSR.select :air @00:04:14.03 CS.seek 4.501ml @00:04:22.66 delay 2 seconds @00:04:24.68 CSV.dial CTV @00:04:25.20 CS.seek 1.275ml @00:04:34.52 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @00:04:34.53 CH.seek 85C,8:00 @00:08:17.39 CH.hold 5:00,85C @00:13:17.55 Collection Puck Heater at 84.9C after lysing @00:13:17.57 CS.seek 0ml @00:13:21.89 CTV.dial between spare and air,avoiding: :intake @00:13:23.08 CSV.dial :air @00:13:23.57 CS.seek 5ml @00:13:37.39 CSV.dial CTV @00:13:37.89 delay 2 seconds @00:13:39.91 CSV.dial :air @00:13:40.53 delay 2 seconds @00:13:42.55 CSV.dial CTV @00:13:43.05 delay 2 seconds @00:13:45.07 CSV.dial :air @00:13:45.59 delay 2 seconds @00:13:47.62 CS.seek 0ml @00:14:01.33 Recovering Lysate into Collection syringe @00:14:01.34 CSV.dial CBV @00:14:02.50 CBV.dial CSV @00:14:02.90 CS.seek 5ml @00:14:16.73 delay 5 seconds @00:14:21.75 CTV.dial :air @00:14:22.34 CS.seek 7ml @00:14:28.51 delay 5 seconds @00:14:33.53 CSV.dial :air2 @00:14:34.03 CS.seek 10ml @00:14:42.73 CSV.dial between air and CTV,avoiding: CBV @00:14:44.03 Lysing Complete @00:14:44.10 Storing sh1 puck in Collection Clamp to tube 1 @00:14:44.26 SC.reconfigure SCtight @00:14:44.30 SC.seek 1 @00:14:46.45 SC.reconfigure SCloose @00:14:47.07 Elbow.reconfigure TightElbow @00:14:47.14 Elbow.seek 1 @00:14:49.03 Forearm.reconfigure TightForearm @00:14:49.07 Forearm.seek 1 + 700 counts @00:14:50.13 SE.reconfigure SEpick @00:14:50.20 SE.seek beamBreak @00:14:53.19 SE.configure SEconfig @00:14:53.24 SE.seek :place @00:14:53.75 Forearm.reconfigure LooseForearm @00:14:53.79 Forearm.seek :retract @00:14:54.72 Elbow.seek CC @00:14:56.31 Elbow.reconfigure LooseElbow @00:14:56.40 Forearm.seek CC @00:14:57.38 Hand.close @00:14:58.10 CC.seek :open @00:15:02.55 Forearm.seek :retract @00:15:03.54 Elbow.reconfigure TightElbow @00:15:03.58 Elbow.seek 1 @00:15:05.45 Elbow.reconfigure LimpElbow @00:15:05.50 Forearm.seek 1 @00:15:06.51 Elbow.hold @00:15:06.57 Elbow.reconfigure TightElbow @00:15:06.61 Hand.open @00:15:07.47 Forearm.force -20 @00:15:07.50 Forearm.seek 1 + 341 counts @00:15:07.82 Forearm.reconfigure TightForearm @00:15:07.86 Forearm.seek 1 + 700 counts @00:15:08.22 SE.seek :down @00:15:09.34
Forearm.reconfigure LooseForearm @00:15:09.39 Forearm.seek :retract @00:15:11.04 SE.coast @00:15:11.08
SC.coast @00:15:11.11 Tranferring lysate to PRV @00:15:11.12 CSV.dial :air @00:15:11.45 CS.seek 10ml @00:15:11.51 CSV.dial between air and CTV @00:15:11.97 AIV.dial :bypass @00:15:12.05 Beginning samplePassC @00:15:12.06 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @00:15:12.13 Hand.open @00:15:12.48 Elbow.seek FlushPuck::Garage @00:15:14.54 Elbow.reconfigure LooseElbow @00:15:14.63 Forearm.seek :garage @00:15:15.63 Hand.close @00:15:16.47 Forearm.seek :retract @00:15:17.47 Elbow.reconfigure TightElbow @00:15:17.51 Elbow.seek CC @00:15:20.42 Elbow.reconfigure LooseElbow @00:15:20.51 Forearm.reconfigure TightForearm @00:15:20.55 Forearm.seek CC @00:15:22.24 CC.seek :closed @00:15:26.51 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.34 @00:15:26.57 Hand.open @00:15:27.41 Forearm.reconfigure LooseForearm @00:15:27.45 Forearm.seek :retract @00:15:28.41 AWV.select :air @00:15:28.42 AIV.dial :bypass @00:15:28.43 PSV.dial PSR @00:15:29.96 Pulling 1.5ml of PSR.flush @00:15:29.97 PSR.select :flush @00:15:30.10 PS.seek 1.5ml @00:15:46.59 delay 5 seconds @00:15:51.61 PSV.dial :waste1 @00:15:52.14 PS.seek 0ml @00:16:08.53 PSV.dial PSR @00:16:09.03 PSR.select :air @00:16:09.14 PS.seek 2.5ml @00:16:36.00 PRV.dial CBV @00:16:36.03
CBV.dial PRV @00:16:36.92 PSV.dial :mixloop @00:16:38.08 CTV.dial :air,avoiding: :intake @00:16:38.09 PTV.dial :PRVmixing @00:16:38.18 PS.seek 0ml @00:17:04.88 PSV.dial :air @00:17:05.39 PS.seek 2.5ml @00:17:31.97 PSV.dial :mixloop @00:17:32.48 PS.seek 0ml @00:17:59.03 PSV.dial :air @00:17:59.54 PS.seek 2.5ml @00:18:26.03 PSV.dial :mixloop @00:18:26.54 PS.seek 0ml @00:18:52.97 Garaging FlushPuck in Collection Clamp @00:18:53.04 Elbow.reconfigure TightElbow @00:18:53.08 Elbow.seek CC @00:18:53.32 Elbow.reconfigure LooseElbow @00:18:53.40 Forearm.seek CC @00:18:54.37 Hand.close @00:18:55.11 CC.seek :open @00:18:59.44 Forearm.seek :retract @00:19:00.42 Elbow.reconfigure TightElbow @00:19:00.46 Elbow.seek :garage @00:19:03.61 Elbow.reconfigure LooseElbow @00:19:03.70 Forearm.reconfigure TightForearm @00:19:03.74 Forearm.seek :garage @00:19:04.90 Hand.open @00:19:05.67 Forearm.reconfigure LooseForearm @00:19:05.71 Forearm.seek :retract @00:19:06.78 Connecting PS to CS to transfer sample @00:19:06.79 CBV.dial AIV @00:19:06.80 AIV.dial :bypass @00:19:06.81 PSV.dial :mixloop @00:19:06.82 PTV.dial :PRVmixing @00:19:06.83 PRV.dial AIV @00:19:06.84 CTV.dial :air,avoiding: :intake @00:19:06.86 Loading lyfil puck from tube 2 into Collection Clamp @00:19:07.07 SC.reconfigure SCtight @00:19:07.11 SC.seek 2 @00:19:09.72 SC.reconfigure SCloose @00:19:09.92 Elbow.reconfigure TightElbow @00:19:09.97 Elbow.seek 2 @00:19:11.98 Forearm.reconfigure TightForearm @00:19:12.02 Forearm.seek 2 + 700 counts @00:19:13.06 SE.reconfigure SEpick @00:19:13.13 SE.seek beamBreak @00:19:16.24 SE.configure SEconfig @00:19:16.38 Elbow.seek 2 @00:19:16.65 Forearm.seek 2 + 300 counts @00:19:16.92 Forearm.reconfigure LooseForearm @00:19:16.96 Hand.close @00:19:17.75 SE.seek :down @00:19:17.94
Forearm.seek :retract @00:19:18.89 Elbow.seek CC @00:19:20.54 Elbow.reconfigure LooseElbow @00:19:20.63 Forearm.reconfigure TightForearm @00:19:20.69 SE.coast @00:19:20.71
Forearm.seek CC @00:19:22.00 CC.seek :closed @00:19:26.26 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.44 @00:19:26.27 Hand.open @00:19:27.04 Forearm.reconfigure LooseForearm @00:19:27.08 Forearm.seek :retract @00:19:28.04 SC.coast @00:19:28.07 CTV.dial CSV,avoiding: :intake @00:19:29.58 CSV.dial CTV @00:19:29.94 CS.seek 5ml @00:19:43.74 delay 1:20 @00:21:03.77 CS.seek 2.5ml @00:21:11.08 delay 40 seconds @00:21:51.11 CS.seek 0ml @00:21:58.46 delay 1:00 @00:22:58.48 PSV.dial :air @00:22:59.00 PS.seek 2.5ml @00:23:25.85 PSV.dial :mixloop @00:23:26.35 delay 3 seconds @00:23:29.37 PSV.dial :air @00:23:29.85 delay 2 seconds @00:23:31.87 PSV.dial :mixloop @00:23:32.35 delay 3 seconds @00:23:35.37 PSV.dial :waste1 @00:23:36.83 PS.seek :empty @00:24:03.69 CBV.dial :waste1 @00:24:04.21 delay 5 seconds @00:24:09.23 CBV.dial AIV @00:24:09.74 delay 10 seconds @00:24:19.77 CBV.dial :waste1 @00:24:20.29 delay 10 seconds @00:24:30.33 CBV.dial AIV @00:24:30.85 delay 10 seconds @00:24:40.88 CTV.dial CSV,avoiding: :intake @00:24:40.89 CBV.dial between AIV and waste1 @00:24:41.26 CSV.dial :air @00:24:41.78 CS.seek 10ml @00:25:08.27 CSV.dial CTV @00:25:08.78 CS.seek 0ml @00:25:35.65 CBV.dial :waste1 @00:25:36.41 delay 10 seconds @00:25:46.44 CTV.dial :air,avoiding: :intake @00:25:48.02 Storing lyfil puck in Collection Clamp to tube 1 @00:25:48.18 SC.reconfigure SCtight @00:25:48.22 SC.seek 1 @00:25:50.35 SC.reconfigure SCloose @00:25:50.54 Elbow.reconfigure TightElbow @00:25:50.59 Elbow.seek 1 @00:25:52.51 Forearm.reconfigure TightForearm @00:25:52.55 Forearm.seek 1 + 700 counts @00:25:53.59 SE.reconfigure SEpick @00:25:53.66 SE.seek beamBreak @00:25:56.84 SE.configure SEconfig @00:25:56.98 Elbow.reconfigure LimpElbow @00:25:57.02 Forearm.reconfigure LooseForearm @00:25:57.07 Forearm.seek 1 @00:25:57.33 Elbow.hold @00:25:57.40 Elbow.reconfigure TightElbow @00:25:57.49 SE.seek 0.04 Pucks below Top Plate @00:25:57.90 Forearm.force -20 @00:25:57.93 Forearm.seek 1 + 347 counts @00:25:58.26 Forearm.reconfigure TightForearm @00:25:58.30 Forearm.seek 1 + 700 counts @00:25:58.72 SE.seek 1.04 Pucks below Top Plate @00:25:59.29 Forearm.reconfigure LooseForearm @00:25:59.33 Forearm.seek :retract @00:26:00.27 Elbow.seek CC @00:26:01.94 Elbow.reconfigure LooseElbow @00:26:02.03 Forearm.seek CC @00:26:02.99 Hand.close @00:26:03.74 CC.seek :open @00:26:08.21 Forearm.seek :retract @00:26:09.19 Elbow.reconfigure TightElbow @00:26:09.23 Elbow.seek 1 @00:26:11.10 Elbow.reconfigure LimpElbow @00:26:11.15 Forearm.seek 1 @00:26:12.14 Elbow.hold @00:26:12.20 Elbow.reconfigure TightElbow @00:26:12.24 Hand.open @00:26:13.11 Forearm.force -20 @00:26:13.14 Forearm.seek 1 + 346 counts @00:26:13.47 Forearm.reconfigure TightForearm @00:26:13.52 Forearm.seek 1 + 700 counts @00:26:13.91 SE.seek :down @00:26:15.05
Forearm.reconfigure LooseForearm @00:26:15.09 Forearm.seek :retract @00:26:16.59 SE.coast @00:26:16.63
SC.coast @00:26:16.66 PSV.dial :air @00:26:17.86 PS.seek 0.499ml @00:26:23.83 delay 3 seconds @00:26:26.85 PSV.dial :mixloop @00:26:27.33 PRV.dial :air @00:26:27.85 delay 3 seconds @00:26:30.87 PRV.dial CBV @00:26:31.38 CBV.dial AIV @00:26:31.90 PRV.dial between CBV and air @00:26:32.26 PRV.dial :air @00:26:32.64 PS.reconfigure PSslow @00:26:32.68 PS.seek 0.599ml @00:26:35.22 PRV.dial :waste2 @00:26:36.05 PS.reconfigure PSconfig @00:26:36.09 PS.seek :empty @00:26:43.10 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @00:26:43.16 Hand.open @00:26:43.47 Elbow.seek FlushPuck::Garage @00:26:45.49 Elbow.reconfigure LooseElbow @00:26:45.58 Forearm.seek :garage @00:26:46.60 Hand.close @00:26:47.47 Forearm.seek :retract @00:26:48.48 Elbow.reconfigure TightElbow @00:26:48.53 Elbow.seek CC @00:26:51.36 Elbow.reconfigure LooseElbow @00:26:51.44 Forearm.reconfigure TightForearm @00:26:51.48 Forearm.seek CC @00:26:52.73 CC.seek :closed @00:26:56.98 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.34 @00:26:57.04 Hand.open @00:26:57.80 Forearm.reconfigure LooseForearm @00:26:57.84 Forearm.seek :retract @00:26:58.91 Clearing PML for modified lysate @00:26:58.92 CTV.dial :air,avoiding: :intake @00:26:58.93 PRV.dial :waste2 @00:26:58.94 PTV.dial :PRV,avoiding: :puck @00:26:59.76 delay 2 seconds @00:27:01.78 PTV.dial :PRVmixing,avoiding: :puck @00:27:02.60 delay 2 seconds @00:27:04.62 PSV.dial :PML @00:27:04.64 delay 2 seconds @00:27:06.66 PRV.dial :air @00:27:07.49 delay 2 seconds @00:27:09.57 PS.seek 1.499ml @00:27:26.07 delay 5 seconds @00:27:31.10 PS.seek 2.5ml @00:27:42.26 PSV.dial :air @00:27:42.77 PS.seek 0ml @00:28:09.44 mixing lysate with speDil @00:28:09.45 PSV.dial :PML @00:28:09.94 CBV.dial :PRV @00:28:09.95 PRV.dial :air @00:28:09.96 delay 2 seconds @00:28:11.98 PRV.dial :speDil @00:28:12.49 delay 2 seconds @00:28:14.57 PS.reconfigure PSslow1 @00:28:14.61 PS.seek 0.225ml @00:28:38.60 delay 5 seconds @00:28:43.62 PRV.dial CBV @00:28:44.48 delay 2 seconds @00:28:47.00 PS.seek 0.475ml @00:29:12.82 delay 5 seconds @00:29:17.84 PRV.dial :air @00:29:18.37 delay 2 seconds @00:29:20.40 PS.seek 0.965ml @00:30:10.60 delay 10 seconds @00:30:20.63 PRV.dial CBV @00:30:21.15 CTV.dial :air,avoiding: :intake @00:30:21.16 PSV.dial :air @00:30:21.77 PS.reconfigure PSconfig @00:30:21.82 PS.seek 2.5ml @00:30:38.48 delay 3 seconds @00:30:41.50 PSV.dial PTV @00:30:42.43 PTV.dial :PRV,avoiding: :puck @00:30:43.25 PS.seek 0ml @00:31:10.20 PSV.dial :air @00:31:11.00 PS.seek 2.5ml @00:31:37.41 delay 3 seconds @00:31:40.43 PSV.dial PTV @00:31:41.37 PTV.dial :PRV,avoiding: :puck @00:31:41.39 PS.seek 0ml @00:32:08.04 Pushing modified lysate into AIV Injection Loop @00:32:08.05 CBV.dial AIV @00:32:08.06 AIV.dial :bypass @00:32:08.07 PRV.dial CBV @00:32:08.08 PSV.dial :air @00:32:08.89 PS.seek 2.5ml @00:32:35.35 delay 3 seconds @00:32:38.37 PSV.dial :PML @00:32:38.85 PTV.dial :PRVmixing,avoiding: :puck @00:32:39.74 PS.reconfigure PSslow1 @00:32:39.78 PS.seek 1.74ml @00:33:57.23 delay 5 seconds @00:34:02.25 AIV.dial :sample @00:34:02.89 AWV.select :waste @00:34:03.04 PS.reconfigure PSslow2 @00:34:03.09 PS.seek 1.22ml @00:35:50.67 delay 15 seconds @00:36:05.69 AIV.dial :bypass @00:36:06.31 PS.reconfigure PSconfig @00:36:06.35 PS.seek 0ml @00:36:19.73 PSV.dial :air @00:36:20.24 PS.seek 2.5ml @00:36:46.85 PSV.dial :PML @00:36:47.36 PS.seek 0ml @00:37:13.98 AIV.dial :coilToMFB @00:37:13.99 AWV.select :air @00:37:14.14 Extracting Nucleic Acid from AIV @00:37:14.17 SPE.hold 2:46:40,55C @00:37:14.19
Beginning samplePassD @00:37:14.26 ASV.dial :SPEdelivery @00:37:14.33
AWV.select :air @00:37:14.34 AIV.dial :bypass @00:37:14.36 PSV.dial PSR @00:37:14.69 delay 20 seconds @00:37:15.62
Pulling 1.5ml of PSR.killMFB @00:37:15.63 PSR.select :killMFB @00:37:15.76 PS.seek 1.5ml @00:37:32.89 delay 5 seconds @00:37:34.71 primeLysateINT @00:37:34.72 AWV.select :air @00:37:34.73 AIV.dial :coilToMFB @00:37:34.75 ATV.dial AIV @00:37:36.63 AS.reconfigure ASslow1 @00:37:36.67 AS.seek 0.299ml @00:37:37.91
PSV.dial :waste1 @00:37:38.44 PS.seek 0ml @00:37:55.48 PSV.dial PSR @00:37:56.01 PSR.select :air @00:37:56.13 PS.seek 2.5ml @00:38:24.14 PRV.dial CBV @00:38:24.16
CBV.dial PRV @00:38:24.18 PSV.dial :mixloop @00:38:25.39 CTV.dial :air,avoiding: :intake @00:38:25.40 PTV.dial :PRVmixing @00:38:25.42 PS.seek 0ml @00:38:37.38 delay 10 seconds @00:38:47.41 ATV.dial :air2 @00:38:48.74 AS.seek 0.329ml @00:38:53.20
PSV.dial PSR @00:38:54.46 Pulling 1.5ml of PSR.flush @00:38:54.47 PSR.select :flush @00:38:54.59 PS.seek 1.5ml @00:38:55.04 ATV.dial :waste2 @00:38:55.63 AS.reconfigure ASnorm @00:38:55.68 AS.seek :empty @00:39:11.67
delay 5 seconds @00:39:12.69 Lysate primed! @00:39:12.70 AFV.select :holdingCoil @00:39:12.71 ATV.dial :air2 @00:39:13.36 AS.reconfigure ASslow1 @00:39:13.40 AS.seek 0.069ml @00:39:16.69
PSV.dial :waste1 @00:39:17.22 PS.seek 0ml @00:39:27.83 delay 3 seconds @00:39:30.85 ATV.dial :AIV @00:39:31.74 AS.seek 0.469ml @00:39:34.29
PSV.dial PSR @00:39:34.82 PSR.select :air @00:39:34.94 PS.seek 2.5ml @00:40:02.76 PRV.dial CBV @00:40:02.78
CBV.dial PRV @00:40:02.80 PSV.dial :mixloop @00:40:03.98 CTV.dial :air,avoiding: :intake @00:40:03.99 PTV.dial :PRVmixing @00:40:04.01 PS.seek 0ml @00:40:32.01 PSV.dial PSR @00:40:33.26 Pulling 1.5ml of PSR.flush PSR.select :flush @00:40:33.39 PS.seek 1.5ml @00:40:50.20 delay 5 seconds @00:40:52.44 delay 5 seconds @00:40:55.22
PSV.dial :waste1 @00:40:55.74 PS.seek 0ml @00:40:57.51 Solid Phase Extraction Heater at 48.1C @00:40:57.52 ATV.dial ASV @00:40:57.93 ASV.dial :SPEdelivery @00:40:58.00 AS.seek 0.041ml @00:41:12.61
PSV.dial PSR @00:41:13.12 PSR.select :air @00:41:13.24 PS.seek 2.5ml @00:41:40.64 PRV.dial CBV @00:41:40.66
CBV.dial PRV @00:41:40.68 PSV.dial :mixloop @00:41:41.86 CTV.dial :air,avoiding: :intake @00:41:41.87 PTV.dial :PRVmixing @00:41:41.89 PS.seek 0ml @00:42:09.11 PSV.dial PSR @00:42:10.33 Pulling 1.5ml of PSR.flush @00:42:10.34 PSR.select :flush @00:42:10.47 PS.seek 1.5ml @00:42:24.32 delay 10 seconds @00:42:27.08
delay 5 seconds @00:42:32.10 PSV.dial :waste1 @00:42:32.62 PS.seek 0ml @00:42:34.34 rinseLysateLineINT @00:42:34.35 AFV.select :carrierSolution @00:42:34.45 delay 2 seconds @00:42:36.48 AS.reconfigure ASconfig @00:42:36.52 AS.seek 1ml @00:42:49.26
PSV.dial PSR @00:42:49.78 PSR.select :air @00:42:49.90 PS.seek 2.5ml @00:43:16.80 PRV.dial CBV @00:43:16.82
CBV.dial PRV @00:43:16.84 PSV.dial :mixloop @00:43:18.00 CTV.dial :air,avoiding: :intake @00:43:18.01 PTV.dial :PRVmixing @00:43:18.03 PS.reconfigure PSslow @00:43:18.07 PS.seek 1.2ml @00:43:31.90 delay 1 second @00:43:32.92 AFV.select :holdingCoil @00:43:33.02 delay 1 second @00:43:34.04 ATV.dial AIV @00:43:34.60 AIV.dial :coilToMFB @00:43:34.61 AWV.select :waste @00:43:34.72 AS.reconfigure ASslow0 @00:43:34.76 AS.seek 0ml @00:43:44.86
PSV.dial :waste1 @00:43:46.75 PS.reconfigure PSconfig @00:43:46.85 PS.seek 0ml @00:44:00.14 PSV.dial :air @00:44:01.32 PS.seek 2.5ml @00:44:28.17 PSV.dial :mixloop @00:44:28.66 PS.reconfigure PSslow @00:44:28.70 PS.seek 0ml @00:45:15.65 ATV.dial :air2 @00:45:16.32 AS.reconfigure ASslow1 @00:45:16.36 AS.seek 0.05ml @00:45:19.77
PSV.dial :air @00:45:20.27 PS.reconfigure PSconfig @00:45:20.32 PS.seek 2.5ml @00:45:26.71 ATV.dial :AIV @00:45:27.54 AS.seek 0.01ml @00:45:35.87 ATV.dial :waste @00:45:37.54 AS.seek :empty @00:45:39.93 ethanol wash @00:45:39.94 ATV.dial :air2 @00:45:41.23 AS.reconfigure ASslow2 @00:45:41.27 AS.seek 0.009ml @00:45:47.23
PSV.dial :mixloop @00:45:47.73 PS.reconfigure PSslow @00:45:47.78 PS.seek 0ml @00:45:51.95 delay 3 seconds @00:45:54.97 Pulling 0.4ml of Ethanol Wash @00:45:54.98 ATV.dial :ethanol @00:45:55.82 AS.reconfigure ASslow1 @00:45:55.86 AS.seek 0.409ml @00:46:38.99
Cleaning Collection Syringe @00:46:39.00 CSV.dial CSR @00:46:39.54 Pulling 4ml of CSR.flush CSR.select :flush @00:46:39.67 CS.seek 4ml @00:46:51.01 delay 5 seconds @00:46:56.03 CSR.select :air @00:46:56.15 CS.seek 10ml @00:47:12.54 CSV.dial CTV @00:47:12.57
CTV.dial CSV @00:47:14.15 CBV.dial :waste1 @00:47:14.68 CS.seek 0ml @00:47:16.55 delay 3 seconds @00:47:19.57 ATV.dial :air1 @00:47:19.96 delay 3 seconds @00:47:23.03 AS.seek 0.434ml @00:47:28.34 delay 3 seconds @00:47:31.36 ATV.dial :ethanol @00:47:31.97 AS.seek 0.419ml @00:47:35.30 delay 3 seconds @00:47:38.32 ATV.dial ASV @00:47:39.66 AS.seek 0.092ml @00:47:41.23
CSV.dial CSR @00:47:41.79 Pulling 4ml of CSR.flush CSR.select :flush @00:47:41.92 CS.seek 4ml @00:47:53.32 delay 5 seconds @00:47:58.34 CSR.select :air @00:47:58.46 CS.seek 10ml @00:48:14.76 CSV.dial CTV @00:48:14.78
CTV.dial CSV @00:48:15.29 CBV.dial :waste1 @00:48:15.31 CS.seek 0ml @00:48:41.95 Garaging FlushPuck in Collection Clamp @00:48:42.01 Elbow.reconfigure TightElbow @00:48:42.05 Elbow.seek CC @00:48:42.31 Elbow.reconfigure LooseElbow @00:48:42.39 Forearm.seek CC @00:48:43.35 Hand.close @00:48:44.14 CC.seek :open @00:48:45.68 delay 3 seconds @00:48:48.50
Forearm.seek :retract @00:48:48.70 ATV.dial :waste2 @00:48:49.50
Elbow.reconfigure TightElbow @00:48:49.54 Elbow.seek :garage @00:48:49.93 AS.reconfigure ASconfig @00:48:49.98 AS.seek :empty @00:48:52.67
Elbow.reconfigure LooseElbow @00:48:52.76 Forearm.reconfigure TightForearm @00:48:52.80 Forearm.seek :garage @00:48:53.96 Hand.open @00:48:54.82 Forearm.reconfigure LooseForearm @00:48:54.86 Forearm.seek :retract @00:48:55.62 ATV.dial :air2 @00:48:55.89
Skipping Whole Cell Archive @00:48:55.90 Starting finalPurge @00:48:55.91 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @00:48:55.99 Hand.open @00:48:56.03 AS.reconfigure ASslow1 @00:48:56.09 AS.seek 1ml @00:48:56.32
Elbow.reconfigure TightElbow @00:48:56.37 Elbow.seek FlushPuck::Garage @00:48:56.49 Elbow.reconfigure LooseElbow @00:48:56.58 Forearm.seek :garage @00:48:57.59 Hand.close @00:48:58.51 Forearm.seek :retract @00:48:59.52 Elbow.reconfigure TightElbow @00:48:59.56 Elbow.seek CC @00:49:02.48 Elbow.reconfigure LooseElbow @00:49:02.57 Forearm.reconfigure TightForearm @00:49:02.61 Forearm.seek CC @00:49:03.85 CC.seek :closed @00:49:08.10 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.33 @00:49:08.17 Hand.open @00:49:08.95 Forearm.reconfigure LooseForearm @00:49:08.99 Forearm.seek :retract @00:49:10.00 Flushing the Collection Valve::Manifold @00:49:10.12 CSV.dial CSR @00:49:10.67 Pulling 5ml of CSR.flush @00:49:10.68 CSR.select :flush @00:49:10.81 CS.seek 5ml @00:49:24.64 delay 5 seconds @00:49:29.66 CSR.select :air @00:49:29.82 CS.reconfigure CSfast @00:49:29.86 CS.seek 7.5ml @00:49:37.20 CSV.dial :waste @00:49:38.79 CS.reconfigure CSconfig @00:49:38.84 CS.seek 0ml @00:49:59.23 Flushing lower manifold's waste1 port @00:49:59.35 CSV.dial :flush @00:49:59.87 CS.reconfigure CSfast @00:49:59.92 CS.seek 5ml @00:50:13.61 delay 5 seconds @00:50:18.63 CSV.dial :air @00:50:19.18 CS.seek 7ml @00:50:25.29 CSV.dial CTV @00:50:25.32
CTV.dial CSV @00:50:25.93 CBV.dial :waste1 @00:50:25.95 CS.seek 0ml @00:50:45.29 Flushing lower manifold's waste2b port @00:50:45.41 CSV.dial :flush @00:50:46.24 CS.seek 5ml @00:50:59.98 delay 5 seconds @00:51:05.00 CSV.dial :air @00:51:05.58 CS.seek 7ml @00:51:11.73 CSV.dial CTV @00:51:11.76
CTV.dial CSV @00:51:12.26 CBV.dial :waste2b @00:51:13.12 CS.seek 0ml @00:51:31.98 Flushing lower manifold's waste1 port @00:51:31.99 CSV.dial CSR @00:51:32.54 Pulling 4ml of CSR.mfbkill @00:51:32.55 CSR.select :mfbkill @00:51:32.69 CS.reconfigure CSconfig @00:51:32.73 CS.seek 4ml @00:51:44.17 delay 5 seconds @00:51:49.19 CSR.select :air @00:51:49.31 CS.reconfigure CSfast @00:51:49.35 CS.seek 10ml @00:52:05.70 CSV.dial CTV @00:52:05.73
CTV.dial CSV @00:52:06.23 CBV.dial :waste1 @00:52:07.10 CS.seek 0ml @00:52:17.68 delay 5 seconds @00:52:22.70 ATV.dial ASV @00:52:23.70 ASV.dial :SPEdelivery @00:52:23.72 AS.seek 0.02ml @00:52:33.67
Flushing lower manifold's waste2b port @00:52:33.68 CSV.dial CSR @00:52:34.25 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @00:52:34.36 CS.reconfigure CSconfig @00:52:34.40 CS.seek 4ml @00:52:45.67 delay 5 seconds @00:52:50.69 CSR.select :air @00:52:50.81 CS.reconfigure CSfast @00:52:50.85 CS.seek 10ml @00:53:07.14 CSV.dial CTV @00:53:07.17
CTV.dial CSV @00:53:07.69 CBV.dial :waste2b @00:53:08.56 CS.seek 0ml @00:53:35.14 Killing upper manifold @00:53:35.15 CSV.dial CSR @00:53:35.71 Pulling 4ml of CSR.mfbkill @00:53:35.72 CSR.select :mfbkill @00:53:35.84 CS.reconfigure CSconfig @00:53:35.88 CS.seek 4ml @00:53:47.14 delay 5 seconds @00:53:52.16 CSR.select :air @00:53:52.28 CS.reconfigure CSfast @00:53:52.32 CS.seek 10ml @00:54:08.67 CSV.dial CBV @00:54:08.71
CBV.dial CSV @00:54:09.87 CTV.dial :air @00:54:11.40 CS.seek 0ml @00:54:37.89 delay 7 seconds @00:54:44.92 CS.seek 3ml @00:54:53.61 CSV.dial :waste @00:54:54.10 CS.seek 0ml @00:55:02.77 Flushing upper manifold @00:55:02.89 CSV.dial :flush @00:55:03.39 CS.seek 5ml @00:55:17.20 delay 5 seconds @00:55:22.22 CSV.dial :air @00:55:22.76 CS.seek 7.001ml @00:55:28.92 CSV.dial CBV @00:55:28.94
CBV.dial CSV @00:55:30.13 CTV.dial :air @00:55:30.15 CS.seek 0ml @00:55:41.07 delay 5 seconds @00:55:46.09 ATV.dial :air2 @00:55:47.15 AS.seek 0.52ml @00:55:48.83
delay 7 seconds @00:55:55.86 CS.seek 3ml @00:56:04.45 CSV.dial :waste @00:56:04.96 CS.seek 0ml @00:56:14.03 CSV.dial :flush @00:56:14.54 CS.seek 3ml @00:56:23.27 delay 5 seconds @00:56:28.29 CSV.dial :air @00:56:28.84 CS.seek 5ml @00:56:34.89 CSV.dial :air @00:56:34.91 CS.seek 0ml @00:56:48.55 delay 7 seconds @00:56:55.58 CS.seek 3ml @00:57:04.27 CSV.dial :waste @00:57:05.11 CS.seek 0ml @00:57:13.67 Flushing lower manifold's waste1 port @00:57:13.79 CSV.dial :flush @00:57:14.29 CS.seek 5ml @00:57:27.99 delay 5 seconds @00:57:28.11
delay 5 seconds @00:57:33.01 ATV.dial ASV @00:57:33.13
CSV.dial :air @00:57:33.80 CS.seek 7.001ml @00:57:34.01 ASV.dial :wasteR @00:57:34.39 AS.seek 0.3ml @00:57:39.83 CSV.dial CTV @00:57:39.87
CTV.dial CSV @00:57:41.42 CBV.dial :waste1 @00:57:41.95 CS.seek 0ml @00:58:00.67 Flushing lower manifold's waste2b port @00:58:00.79 CSV.dial :flush @00:58:01.62 CS.seek 5ml @00:58:15.30 delay 5 seconds @00:58:18.92 delay 20 seconds @00:58:20.32
CSV.dial :air @00:58:20.89 CS.seek 7.001ml @00:58:27.05 CSV.dial CTV @00:58:27.09
CTV.dial CSV @00:58:27.57 CBV.dial :waste2b @00:58:28.44 CS.seek 0ml @00:58:38.95 AS.seek 0.07ml @00:58:47.21
Garaging FlushPuck in Collection Clamp @00:58:47.29 Elbow.reconfigure TightElbow @00:58:47.33 Elbow.seek CC @00:58:47.58 Elbow.reconfigure LooseElbow @00:58:47.67 Forearm.seek CC @00:58:48.65 Hand.close @00:58:49.40 CC.seek :open @00:58:53.75 Forearm.seek :retract @00:58:54.74 Elbow.reconfigure TightElbow @00:58:54.78 Elbow.seek :garage @00:58:57.89 Elbow.reconfigure LooseElbow @00:58:57.97 Forearm.reconfigure TightForearm @00:58:58.01 Forearm.seek :garage @00:58:59.22 Hand.open @00:58:59.96 Forearm.reconfigure LooseForearm @00:59:00.00 Forearm.seek :retract @00:59:01.04 Starting initialPurge @00:59:01.05 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @00:59:01.11 Hand.open @00:59:01.46 Elbow.reconfigure TightElbow @00:59:01.50 Elbow.seek FlushPuck::Garage @00:59:01.63 Elbow.reconfigure LooseElbow @00:59:01.72 Forearm.seek :garage @00:59:02.72 Hand.close @00:59:04.09 Forearm.seek :retract @00:59:05.10 Elbow.reconfigure TightElbow @00:59:05.15 Elbow.seek CC @00:59:08.05 Elbow.reconfigure LooseElbow @00:59:08.14 Forearm.reconfigure TightForearm @00:59:08.18 Forearm.seek CC @00:59:09.47 CC.seek :closed @00:59:13.73 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.31 @00:59:13.79 Hand.open @00:59:14.58 Forearm.reconfigure LooseForearm @00:59:14.62 Forearm.seek :retract @00:59:15.67 Flushing the Collection Valve::Manifold @00:59:15.79 CSV.dial CSR @00:59:16.32 Pulling 5ml of CSR.flush @00:59:16.33 CSR.select :flush @00:59:16.45 CS.reconfigure CSconfig @00:59:16.49 CS.seek 5ml @00:59:25.46 delay 20 seconds @00:59:30.59
delay 5 seconds @00:59:35.61 CSR.select :air @00:59:35.77 CS.reconfigure CSfast @00:59:35.81 CS.seek 7.501ml @00:59:43.15 CSV.dial :waste @00:59:44.65 CS.reconfigure CSconfig @00:59:44.70 CS.seek 0ml @00:59:45.48 ATV.dial :waste2 @00:59:46.24 AS.reconfigure ASconfig @00:59:46.29 AS.seek :empty @00:59:50.68 Elution @00:59:50.69 ATV.dial :air1 @00:59:51.80 AS.reconfigure ASslow1 @00:59:51.84 AS.seek 0.049ml @01:00:02.21 delay 3 seconds @01:00:04.93
Purging external Intake @01:00:05.04 CSV.dial :flush @01:00:05.23 ATV.dial :ElutionWater @01:00:05.55
CS.reconfigure CSfast @01:00:05.60 CS.seek 10ml @01:00:05.91 AS.seek 0.109ml @01:00:18.23 delay 3 seconds @01:00:21.25 ATV.dial ASV,via: :air2 @01:00:23.40 ASV.dial :SPEelution @01:00:24.00 AFV.select :carrierSolution @01:00:24.15 AS.reconfigure ASnorm @01:00:24.19 AS.seek 0.759ml @01:00:32.40
delay 5 seconds @01:00:37.42 CSV.dial CBV @01:00:37.46
CBV.dial CSV @01:00:38.49 CTV.dial :intake @01:00:39.33 Intake.select :open @01:00:39.44 CS.seek 0ml @01:00:57.24 delay 3 seconds @01:01:00.26 AFV.select :holdingCoil @01:01:00.41 AS.reconfigure ASslow1 @01:01:00.45 AS.seek 0.474ml @01:01:06.71
Intake.select :close @01:01:06.81 CTV.dial :air @01:01:07.68 Flushing upper manifold @01:01:07.79 CSV.dial :flush @01:01:08.61 CS.seek 5ml @01:01:22.74 delay 5 seconds @01:01:27.76 CSV.dial :air @01:01:28.32 CS.seek 7ml @01:01:34.62 CSV.dial CBV @01:01:34.65
CBV.dial CSV @01:01:35.93 CTV.dial :air @01:01:35.95 CS.seek 0ml @01:01:54.55 delay 7 seconds @01:01:58.04 delay 5 seconds @01:02:01.58
CS.seek 3ml @01:02:03.11 AS.reconfigure ASslow2 @01:02:03.15 AS.seek 0.439ml @01:02:10.24
CSV.dial :waste @01:02:10.73 CS.seek 0ml @01:02:19.44 CSV.dial :flush @01:02:19.94 CS.seek 3ml @01:02:28.62 delay 5 seconds @01:02:33.64 CSV.dial :air @01:02:34.19 CS.seek 5ml @01:02:39.06 delay 1:00 @01:02:40.27
CSV.dial :air @01:02:40.29 CS.seek 0ml @01:02:53.96 delay 7 seconds @01:03:00.99 CS.seek 3ml @01:03:09.71 CSV.dial :waste @01:03:10.55 CS.seek 0ml @01:03:19.30 Garaging FlushPuck in Collection Clamp @01:03:19.37 Elbow.reconfigure TightElbow @01:03:19.41 Elbow.seek CC @01:03:19.68 Elbow.reconfigure LooseElbow @01:03:19.77 Forearm.seek CC @01:03:20.74 Hand.close @01:03:21.53 CC.seek :open @01:03:25.92 Forearm.seek :retract @01:03:26.96 Elbow.reconfigure TightElbow @01:03:27.01 Elbow.seek :garage @01:03:30.26 Elbow.reconfigure LooseElbow @01:03:30.35 Forearm.reconfigure TightForearm @01:03:30.39 Forearm.seek :garage @01:03:31.56 Hand.open @01:03:32.34 Forearm.reconfigure LooseForearm @01:03:32.38 Forearm.seek :retract @01:03:33.43 Completed [coreNEG] begun 23:51:05 @01:03:33.44 Extending 18tun until completion of MFBPCR @01:03:39.08 pushing eluate to holding coil @01:03:39.14 AS.seek 0.279ml @01:06:20.37 delay 30 seconds @01:06:50.40 ATV.dial :waste,via: :air3 @01:06:52.14 AS.reconfigure ASconfig @01:06:52.19 AS.seek :empty @01:07:08.54 Parking Bleach in LysateSource line and SPEcolumn @01:07:08.55 ATV.dial :air3 @01:07:09.37 AS.reconfigure ASslow1 @01:07:09.41 AS.seek 0.029ml @01:07:15.72 ATV.dial :ElutionWater @01:07:16.32 AS.seek 0.059ml @01:07:22.62 ATV.dial :air3 @01:07:23.23 AS.seek 0.069ml @01:07:25.48 delay 2 seconds @01:07:27.50 ATV.dial :bleach @01:07:27.95 AS.seek 0.769ml @01:09:48.98 delay 3 seconds @01:09:52.00 ATV.dial :air3 @01:09:52.54 delay 3 seconds @01:09:55.61 AS.seek 0.794ml @01:10:00.90 delay 3 seconds @01:10:03.92 ATV.dial :bleach @01:10:04.53 AS.seek 0.779ml @01:10:07.84 delay 3 seconds @01:10:10.86 ATV.dial :AIV @01:10:12.12 AS.reconfigure ASconfig @01:10:12.17 AS.seek 0.015ml @01:10:56.39 ATV.dial :waste @01:10:57.89 AS.seek :empty @01:10:59.11 ATV.dial :air3 @01:10:59.78 AS.reconfigure ASslow1 @01:10:59.82 AS.seek 0.009ml @01:11:02.15 delay 2 seconds @01:11:04.17 ATV.dial :bleach @01:11:04.62 AS.seek 0.579ml @01:12:59.51 delay 3 seconds @01:13:02.53 ATV.dial :air3 @01:13:03.08 delay 3 seconds @01:13:06.15 AS.seek 0.604ml @01:13:11.44 delay 3 seconds @01:13:14.46 ATV.dial :bleach @01:13:15.53 AS.seek 0.589ml @01:13:18.83 delay 3 seconds @01:13:21.85 ATV.dial :ASV @01:13:22.89 ASV.dial :wasteR @01:13:23.49 AS.seek 0ml @01:15:22.26 delay 1:00 @01:16:22.29 AFV.select :carrierSolution @01:16:22.39 delay 2 seconds @01:16:24.42 AS.reconfigure ASconfig @01:16:24.46 AS.seek 1ml @01:17:22.22 delay 1 second @01:17:23.24 AFV.select :holdingCoil @01:17:23.34 delay 1 second @01:17:24.36 ATV.dial ASV @01:17:24.37 ASV.dial :wasteR @01:17:24.39 AS.reconfigure ASslow1 @01:17:24.43 AS.seek 0ml @01:20:45.81 delay 20 seconds @01:21:05.84 ATV.dial :air3 @01:21:07.36 AS.seek 0.59ml @01:23:06.31 delay 3 seconds @01:23:09.33 ATV.dial :ASV @01:23:10.76 ASV.dial :wasteR @01:23:10.83 AS.seek 0.09ml @01:24:51.67 delay 30 seconds @01:25:21.70 ATV.dial :extSrc @01:25:22.48 delay 3 seconds @01:25:25.50 ATV.dial :ElutionWater @01:25:26.62 AS.seek 0.12ml @01:25:32.94 ATV.dial :air3 @01:25:33.54 AS.seek 0.13ml @01:25:35.73 delay 2 seconds @01:25:37.75 ATV.dial :bleach @01:25:38.21 AS.seek 0.23ml @01:25:58.64 delay 3 seconds @01:26:01.66 ATV.dial :air3 @01:26:02.20 delay 3 seconds @01:26:05.27 AS.seek 0.255ml @01:26:10.53 delay 3 seconds @01:26:13.55 ATV.dial :bleach @01:26:14.15 AS.seek 0.24ml @01:26:17.41 delay 3 seconds @01:26:20.43 ATV.dial :ASV @01:26:21.48 ASV.dial :SPEdelivery @01:26:21.86 AFV.select :carrierSolution @01:26:21.96 delay 2 seconds @01:26:23.99 AS.reconfigure ASconfig @01:26:24.03 AS.seek 0.5ml @01:26:39.25 delay 1 second @01:26:40.27 AFV.select :holdingCoil @01:26:40.42 AS.reconfigure ASslow1 @01:26:40.46 AS.seek 0.17ml @01:27:47.14 SPE.hold 2:46:40,95C @01:27:47.18 delay 3:00 @01:30:47.21 AFV.select :carrierSolution @01:30:47.31 delay 2 seconds @01:30:49.34 AS.reconfigure ASconfig @01:30:49.38 AS.seek 1ml @01:31:37.39 delay 1 second @01:31:38.41 AFV.select :holdingCoil @01:31:38.51 delay 1 second @01:31:39.53 ASV.dial :wasteD @01:31:40.62 AS.seek 0ml @01:32:38.39 Priming Eluate @01:32:38.40 AFV.select :carrierSolution @01:32:38.50 delay 2 seconds @01:32:40.53 AS.seek 0.5ml @01:33:09.56 delay 1 second @01:33:10.58 AFV.select :holdingCoil @01:33:10.68 ATV.dial ASV @01:33:10.69 ASV.dial :SPErecovery @01:33:11.30 Swish #1 of 2 @01:33:11.37 AS.reconfigure ASslow0 @01:33:11.41 AS.seek 0.6ml @01:33:21.81 AS.seek 0.5ml @01:33:32.16 Swish #2 of 2 @01:33:32.23 AS.seek 0.6ml @01:33:42.76 AS.seek 0.5ml @01:33:53.13 delay 3 seconds @01:33:56.20 AS.reconfigure ASslow1 @01:33:56.24 AS.seek 0.684ml @01:34:33.66 delay 3 seconds @01:34:36.68 Eluate primed @01:34:36.69 ATV.dial :waste2 @01:34:37.31 AS.reconfigure ASnorm @01:34:37.36 AS.seek 0ml @01:35:12.14 SPE.coast @01:35:12.20 ASV.dial :SPErecovery @01:35:12.22 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118aug2901h.pcr @01:35:12.24 Building PCR Slug from ASV with MM2 mix, PP4 primer @01:35:12.25 Priming with carrier @01:35:12.26 ATV.dial ARV @01:35:13.22 ARV.dial :carrier @01:35:13.56 AS.seek 0.5ml @01:35:39.13 AFV.select :carrierSolution @01:35:39.23 delay 2 seconds @01:35:41.26 AS.reconfigure ASconfig @01:35:41.30 AS.seek 1ml @01:36:10.33 delay 1 second @01:36:11.75 AFV.select :holdingCoil @01:36:11.91 delay 1 second @01:36:12.93 ATV.dial :waste @01:36:14.42 AS.reconfigure ASnorm @01:36:14.47 AS.seek :empty @01:37:05.21 AFV.select :carrierSolution @01:37:05.31 delay 2 seconds @01:37:07.34 AS.reconfigure ASconfig @01:37:07.38 AS.seek 0.5ml @01:37:36.47 delay 1 second @01:37:37.49 AFV.select :holdingCoil @01:37:37.60 Building Slug @01:37:37.61 ATV.dial :ARV @01:37:39.24 delay 2 seconds @01:37:41.26 ARV.dial :air1 @01:37:41.67 delay 2 seconds @01:37:43.74 AS.reconfigure ASslow2 @01:37:43.79 AS.seek 0.506ml @01:37:50.03 delay 2 seconds @01:37:52.05 ARV.dial :MM2 @01:37:53.55 delay 2 seconds @01:37:55.57 Adding MM2 @01:37:55.65 AS.seek 0.524ml @01:38:13.99 delay 25 seconds @01:38:39.01 ARV.dial :PP4 @01:38:39.41 Adding PP4 @01:38:39.43 delay 2 seconds @01:38:41.49 AS.seek 0.53ml @01:38:47.96 delay 10 seconds @01:38:57.99 ARV.dial :air3 @01:38:58.38 adding ARV air @01:38:58.39 delay 2 seconds @01:39:00.46 AS.seek 0.536ml @01:39:06.99 delay 5 seconds @01:39:12.01 ARV.dial :carrier @01:39:13.28 delay 7 seconds @01:39:20.36 AS.seek 0.594ml @01:40:18.91 delay 5 seconds @01:40:23.93 ATV.dial :ASV @01:40:24.33 delay 2 seconds @01:40:26.40 AS.seek 0.6ml @01:40:32.61 delay 4 seconds @01:40:36.63 ATV.dial ARV @01:40:37.17 delay 2 seconds @01:40:39.19 ARV.dial :carrier @01:40:39.20 delay 2 seconds @01:40:41.27 AS.seek 0.65ml @01:41:31.80 delay 4 seconds @01:41:35.82 ATV.dial PCR @01:41:36.43 ASV.dial :waste1 @01:41:37.21 ARV.dial :air1 @01:41:37.60 Positioning Slug by 0.324ml @01:41:37.68 AS.seek 0.326ml @01:47:03.68 delay 5 seconds @01:47:08.70 ATV.dial ARV @01:47:09.47 ASV.dial :PCRlock @01:47:09.83 delay 5 seconds @01:47:14.85 Running Tt1 Profile60C300 thermocycling @01:47:14.87 PCR.program Profile60C300 @01:47:14.99 Profile60C300 should complete in < 1:45:21 @01:47:15.00 PCR.run 2:16:41.25 @03:08:03.18 PCR.off @03:08:04.67 PCR for Tt1 completed -- closed /var/log/esp/Tt118aug2901h.pcr @03:08:04.73 Cleaning PCR @03:08:04.74 AFV.select :carrierSolution @03:08:04.84 delay 2 seconds @03:08:06.87 AS.reconfigure ASconfig @03:08:06.91 AS.seek 0.5ml @03:08:17.24 delay 1 second @03:08:18.26 AFV.select :holdingCoil @03:08:18.36 ASV.dial :waste1 @03:08:18.74 ATV.dial :air3 @03:08:20.02 AS.reconfigure ASslow1 @03:08:20.06 AS.seek 0.51ml @03:08:22.36 delay 2 seconds @03:08:24.38 ATV.dial :bleach @03:08:24.99 AS.seek 0.81ml @03:09:25.57 delay 3 seconds @03:09:28.59 ATV.dial :air3 @03:09:29.13 delay 3 seconds @03:09:32.20 AS.seek 0.835ml @03:09:37.52 delay 3 seconds @03:09:40.54 ATV.dial :bleach @03:09:41.14 AS.seek 0.82ml @03:09:44.46 delay 3 seconds @03:09:47.48 ARV.dial :waste @03:09:48.08 delay 3 seconds @03:09:51.10 ATV.dial :ARV @03:09:51.99 AS.reconfigure ASconfig @03:09:52.03 AS.seek 0.47ml @03:10:12.43 AFV.select :carrierSolution @03:10:12.53 delay 2 seconds @03:10:14.56 AS.seek 1ml @03:10:45.32 delay 1 second @03:10:46.34 AFV.select :holdingCoil @03:10:46.44 delay 1 second @03:10:47.47 AS.reconfigure ASfast @03:10:47.51 AS.seek 0ml @03:11:14.87 AFV.select :carrierSolution @03:11:14.97 delay 2 seconds @03:11:17.00 AS.reconfigure ASconfig @03:11:17.04 AS.seek 1ml @03:12:15.29 delay 1 second @03:12:16.31 AFV.select :holdingCoil @03:12:16.41 delay 1 second @03:12:17.44 AS.reconfigure ASfast @03:12:17.48 AS.seek 0ml @03:12:44.84 AFV.select :carrierSolution @03:12:44.94 delay 2 seconds @03:12:46.97 AS.reconfigure ASconfig @03:12:47.01 AS.seek 1ml @03:13:44.80 delay 1 second @03:13:45.82 AFV.select :holdingCoil @03:13:45.92 delay 1 second @03:13:46.95 AS.reconfigure ASfast @03:13:46.99 AS.seek 0ml @03:14:14.30 ARV.dial between carrier and air1 @03:14:14.80 ATV.dial :air3 @03:14:16.08 AS.reconfigure ASslow1 @03:14:16.12 AS.seek 0.01ml @03:14:18.48 delay 2 seconds @03:14:20.50 ATV.dial :bleach @03:14:21.11 AS.seek 0.81ml @03:17:02.26 delay 3 seconds @03:17:05.28 ATV.dial :air3 @03:17:05.82 delay 3 seconds @03:17:08.90 AS.seek 0.835ml @03:17:14.22 delay 3 seconds @03:17:17.24 ATV.dial :bleach @03:17:17.85 AS.seek 0.82ml @03:17:21.09 delay 3 seconds @03:17:24.11 ATV.dial PCR @03:17:24.51 AS.reconfigure ASconfig @03:17:24.56 AS.seek :empty @03:18:12.01 PCR.program :bleachProfile @03:18:12.06 PCR program should complete in < 2:45 @03:18:12.07 PCR.run 10:00 @03:21:13.89 PCR.off @03:21:13.92 AFV.select :carrierSolution @03:21:14.02 delay 2 seconds @03:21:16.05 AS.seek 1ml @03:22:13.84 delay 1 second @03:22:14.86 AFV.select :holdingCoil @03:22:14.96 delay 1 second @03:22:15.99 AS.reconfigure ASfast @03:22:16.03 AS.seek 0ml @03:22:43.35 delay 3 seconds @03:22:46.37 AFV.select :carrierSolution @03:22:46.47 delay 2 seconds @03:22:48.50 AS.reconfigure ASconfig @03:22:48.54 AS.seek 1ml @03:23:46.31 delay 1 second @03:23:47.33 AFV.select :holdingCoil @03:23:47.43 delay 1 second @03:23:48.46 AS.reconfigure ASfast @03:23:48.50 AS.seek 0ml @03:24:15.85 delay 3 seconds @03:24:18.87 AFV.select :carrierSolution @03:24:18.97 delay 2 seconds @03:24:21.00 AS.reconfigure ASconfig @03:24:21.05 AS.seek 1ml @03:25:18.82 delay 1 second @03:25:19.84 AFV.select :holdingCoil @03:25:19.94 delay 1 second @03:25:20.97 AS.reconfigure ASfast @03:25:21.01 AS.seek 0ml @03:25:48.34 delay 3 seconds @03:25:51.36 AFV.select :carrierSolution @03:25:51.46 delay 2 seconds @03:25:53.49 AS.reconfigure ASconfig @03:25:53.53 AS.seek 1ml @03:26:51.29 delay 1 second @03:26:52.31 AFV.select :holdingCoil @03:26:52.41 delay 1 second @03:26:53.44 AS.reconfigure ASfast @03:26:53.48 AS.seek 0ml @03:27:20.84 delay 3 seconds @03:27:23.86 AFV.select :carrierSolution @03:27:23.96 delay 2 seconds @03:27:25.99 AS.reconfigure ASconfig @03:27:26.04 AS.seek 1ml @03:28:23.81 delay 1 second @03:28:24.83 AFV.select :holdingCoil @03:28:24.93 delay 1 second @03:28:25.96 AS.reconfigure ASnorm @03:28:26.00 AS.seek 0ml @03:29:16.73 Setting up PCR for Rp -- writing /var/log/esp/Rp18aug2903h.pcr @03:29:16.74 Building PCR Slug from ASV with MM2 mix, PP3 primer @03:29:16.76 Priming with carrier ATV.dial ARV @03:29:17.46 ARV.dial :carrier @03:29:17.80 AS.seek 0.5ml @03:29:43.37 AFV.select :carrierSolution @03:29:43.47 delay 2 seconds @03:29:45.50 AS.reconfigure ASconfig @03:29:45.54 AS.seek 1ml @03:30:14.54 delay 1 second @03:30:15.56 AFV.select :holdingCoil @03:30:15.66 delay 1 second @03:30:16.68 ATV.dial :waste @03:30:18.32 AS.reconfigure ASnorm @03:30:18.86 AS.seek :empty @03:31:09.60 AFV.select :carrierSolution @03:31:09.70 delay 2 seconds @03:31:11.73 AS.reconfigure ASconfig @03:31:11.77 AS.seek 0.5ml @03:31:40.84 delay 1 second @03:31:41.86 AFV.select :holdingCoil @03:31:41.96 Building Slug @03:31:41.97 ATV.dial :ARV @03:31:43.60 delay 2 seconds @03:31:45.62 ARV.dial :air1 @03:31:46.02 delay 2 seconds @03:31:48.09 AS.reconfigure ASslow2 @03:31:48.14 AS.seek 0.506ml @03:31:54.35 delay 2 seconds @03:31:56.37 ARV.dial :MM2 @03:31:57.87 delay 2 seconds @03:31:59.89 Adding MM2 @03:31:59.96 AS.seek 0.524ml @03:32:18.23 delay 25 seconds @03:32:43.25 ARV.dial :PP3 @03:32:43.64 Adding PP3 @03:32:43.65 delay 2 seconds @03:32:45.72 AS.seek 0.53ml @03:32:51.95 delay 10 seconds @03:33:01.98 ARV.dial :air2 @03:33:02.37 adding ARV air @03:33:02.38 delay 2 seconds @03:33:04.45 AS.seek 0.536ml @03:33:10.87 delay 5 seconds @03:33:15.89 ARV.dial :carrier @03:33:17.18 delay 7 seconds @03:33:24.26 AS.seek 0.594ml @03:34:22.82 delay 5 seconds @03:34:27.84 ATV.dial :ASV @03:34:29.31 delay 2 seconds @03:34:31.38 AS.seek 0.6ml @03:34:37.84 delay 4 seconds @03:34:41.86 ATV.dial ARV @03:34:42.39 delay 2 seconds @03:34:44.41 ARV.dial :carrier @03:34:44.42 delay 2 seconds @03:34:46.49 AS.seek 0.65ml @03:35:36.97 delay 4 seconds @03:35:40.99 ATV.dial PCR @03:35:41.61 ASV.dial :waste1 @03:35:41.62 ARV.dial :air1 @03:35:42.02 Positioning Slug by 0.324ml @03:35:42.10 AS.seek 0.326ml @03:41:08.33 delay 5 seconds @03:41:13.35 ATV.dial ARV @03:41:14.12 ASV.dial :PCRlock @03:41:14.50 delay 5 seconds @03:41:19.53 Running Rp Profile60C300 thermocycling @03:41:19.54 PCR.program Profile60C300 @03:41:19.66 Profile60C300 should complete in < 1:45:21 @03:41:19.67 PCR.run 2:16:41.25 @05:01:49.55 PCR.off @05:01:51.07 PCR for Rp completed -- closed /var/log/esp/Rp18aug2903h.pcr @05:01:51.14 Cleaning PCR @05:01:51.16 AFV.select :carrierSolution @05:01:51.29 delay 2 seconds @05:01:53.32 AS.reconfigure ASconfig @05:01:53.36 AS.seek 0.5ml @05:02:03.69 delay 1 second @05:02:04.71 AFV.select :holdingCoil @05:02:04.81 ASV.dial :waste1 @05:02:05.19 ATV.dial :air3 @05:02:06.46 AS.reconfigure ASslow1 @05:02:06.50 AS.seek 0.51ml @05:02:08.79 delay 2 seconds @05:02:10.81 ATV.dial :bleach @05:02:11.42 AS.seek 0.81ml @05:03:12.00 delay 3 seconds @05:03:15.02 ATV.dial :air3 @05:03:15.56 delay 3 seconds @05:03:18.63 AS.seek 0.835ml @05:03:23.94 delay 3 seconds @05:03:26.96 ATV.dial :bleach @05:03:27.56 AS.seek 0.82ml @05:03:30.87 delay 3 seconds @05:03:33.89 ARV.dial :waste @05:03:34.51 delay 3 seconds @05:03:37.53 ATV.dial :ARV @05:03:38.42 AS.reconfigure ASconfig @05:03:38.46 AS.seek 0.47ml @05:03:58.87 AFV.select :carrierSolution @05:03:58.97 delay 2 seconds @05:04:01.00 AS.seek 1ml @05:04:31.81 delay 1 second @05:04:32.83 AFV.select :holdingCoil @05:04:32.93 delay 1 second @05:04:33.96 AS.reconfigure ASfast @05:04:34.00 AS.seek 0ml @05:05:01.34 AFV.select :carrierSolution @05:05:01.44 delay 2 seconds @05:05:03.47 AS.reconfigure ASconfig @05:05:03.51 AS.seek 1ml @05:06:01.30 delay 1 second @05:06:02.32 AFV.select :holdingCoil @05:06:02.42 delay 1 second @05:06:03.45 AS.reconfigure ASfast @05:06:03.49 AS.seek 0ml @05:06:30.84 AFV.select :carrierSolution @05:06:30.94 delay 2 seconds @05:06:32.97 AS.reconfigure ASconfig @05:06:33.01 AS.seek 1ml @05:07:30.80 delay 1 second @05:07:31.82 AFV.select :holdingCoil @05:07:31.92 delay 1 second @05:07:32.95 AS.reconfigure ASfast @05:07:32.99 AS.seek 0ml @05:08:00.39 ARV.dial between carrier and air1 @05:08:00.89 ATV.dial :air3 @05:08:02.65 AS.reconfigure ASslow1 @05:08:02.69 AS.seek 0.01ml @05:08:04.98 delay 2 seconds @05:08:07.00 ATV.dial :bleach @05:08:07.61 AS.seek 0.81ml @05:10:48.77 delay 3 seconds @05:10:51.79 ATV.dial :air3 @05:10:52.34 delay 3 seconds @05:10:55.41 AS.seek 0.835ml @05:11:00.71 delay 3 seconds @05:11:03.73 ATV.dial :bleach @05:11:04.34 AS.seek 0.82ml @05:11:07.66 delay 3 seconds @05:11:10.68 ATV.dial PCR @05:11:11.08 AS.reconfigure ASconfig @05:11:11.13 AS.seek :empty @05:11:58.58 PCR.program :bleachProfile @05:11:58.63 PCR program should complete in < 2:45 @05:11:58.64 PCR.run 10:00 @05:15:00.45 PCR.off @05:15:00.48 AFV.select :carrierSolution @05:15:00.58 delay 2 seconds @05:15:02.61 AS.seek 1ml @05:16:00.49 delay 1 second @05:16:01.51 AFV.select :holdingCoil @05:16:01.61 delay 1 second @05:16:02.64 AS.reconfigure ASfast @05:16:02.68 AS.seek 0ml @05:16:30.05 delay 3 seconds @05:16:33.07 AFV.select :carrierSolution @05:16:33.17 delay 2 seconds @05:16:35.20 AS.reconfigure ASconfig @05:16:35.25 AS.seek 1ml @05:17:33.02 delay 1 second @05:17:34.04 AFV.select :holdingCoil @05:17:34.14 delay 1 second @05:17:35.17 AS.reconfigure ASfast @05:17:35.21 AS.seek 0ml @05:18:02.69 delay 3 seconds @05:18:05.71 AFV.select :carrierSolution @05:18:05.81 delay 2 seconds @05:18:07.84 AS.reconfigure ASconfig @05:18:07.88 AS.seek 1ml @05:19:05.63 delay 1 second @05:19:06.65 AFV.select :holdingCoil @05:19:06.75 delay 1 second @05:19:07.78 AS.reconfigure ASfast @05:19:07.82 AS.seek 0ml @05:19:35.14 delay 3 seconds @05:19:38.16 AFV.select :carrierSolution @05:19:38.26 delay 2 seconds @05:19:40.29 AS.reconfigure ASconfig @05:19:40.34 AS.seek 1ml @05:20:38.11 delay 1 second @05:20:39.13 AFV.select :holdingCoil @05:20:39.23 delay 1 second @05:20:40.26 AS.reconfigure ASfast @05:20:40.30 AS.seek 0ml @05:21:07.63 delay 3 seconds @05:21:10.65 AFV.select :carrierSolution @05:21:10.75 delay 2 seconds @05:21:12.78 AS.reconfigure ASconfig @05:21:12.83 AS.seek 1ml @05:22:10.60 delay 1 second @05:22:11.62 AFV.select :holdingCoil @05:22:11.72 delay 1 second @05:22:12.75 AS.reconfigure ASnorm @05:22:12.79 AS.seek 0ml @05:23:03.52 Setting up PCR for Ss -- writing /var/log/esp/Ss18aug2905h.pcr @05:23:03.53 Building PCR Slug from ASV with MM1 mix, PP2 primer @05:23:03.54 Priming with carrier @05:23:03.55 ATV.dial ARV @05:23:04.16 ARV.dial :carrier @05:23:04.50 AS.seek 0.5ml @05:23:30.05 AFV.select :carrierSolution @05:23:30.15 delay 2 seconds @05:23:32.18 AS.reconfigure ASconfig @05:23:32.22 AS.seek 1ml @05:24:01.24 delay 1 second @05:24:02.26 AFV.select :holdingCoil @05:24:02.36 delay 1 second @05:24:03.38 ATV.dial :waste @05:24:05.14 AS.reconfigure ASnorm @05:24:05.19 AS.seek :empty @05:24:55.93 AFV.select :carrierSolution @05:24:56.03 delay 2 seconds @05:24:58.06 AS.reconfigure ASconfig @05:24:58.10 AS.seek 0.5ml @05:25:27.19 delay 1 second @05:25:28.21 AFV.select :holdingCoil @05:25:28.31 Building Slug @05:25:28.32 ATV.dial :ARV @05:25:29.93 delay 2 seconds @05:25:31.95 ARV.dial :air1 @05:25:32.36 delay 2 seconds @05:25:34.43 AS.reconfigure ASslow2 @05:25:34.48 AS.seek 0.506ml @05:25:40.96 delay 2 seconds @05:25:43.47 ARV.dial :MM1 @05:25:44.07 delay 2 seconds @05:25:46.09 Adding MM1 @05:25:46.16 AS.seek 0.524ml @05:26:04.57 delay 25 seconds @05:26:29.59 ARV.dial :PP2 @05:26:29.99 Adding PP2 @05:26:30.00 delay 2 seconds @05:26:32.07 AS.seek 0.53ml @05:26:38.44 delay 10 seconds @05:26:48.47 ARV.dial :air2 @05:26:48.86 adding ARV air @05:26:48.87 delay 2 seconds @05:26:50.95 AS.seek 0.536ml @05:26:57.33 delay 5 seconds @05:27:02.35 ARV.dial :carrier @05:27:03.64 delay 7 seconds @05:27:10.72 AS.seek 0.594ml @05:28:09.41 delay 5 seconds @05:28:14.43 ATV.dial :ASV @05:28:14.83 delay 2 seconds @05:28:16.90 AS.seek 0.6ml @05:28:23.29 delay 4 seconds @05:28:27.31 ATV.dial ARV @05:28:27.86 delay 2 seconds @05:28:29.88 ARV.dial :carrier @05:28:29.89 delay 2 seconds @05:28:31.96 AS.seek 0.65ml @05:29:22.52 delay 4 seconds @05:29:26.54 ATV.dial PCR @05:29:27.15 ASV.dial :waste1 @05:29:27.16 ARV.dial :air1 @05:29:27.56 Positioning Slug by 0.324ml @05:29:27.64 AS.seek 0.326ml @05:34:53.91 delay 5 seconds @05:34:58.93 ATV.dial ARV @05:34:59.71 ASV.dial :PCRlock @05:35:00.08 delay 5 seconds @05:35:05.10 Running Ss Profile60C350 thermocycling @05:35:05.12 PCR.program Profile60C350 @05:35:05.24 Profile60C350 should complete in < 1:48:05 @05:35:05.25 PCR.run 2:20:06.25 @06:56:11.13 PCR.off @06:56:13.12 PCR for Ss completed -- closed /var/log/esp/Ss18aug2905h.pcr @06:56:13.18 Cleaning PCR @06:56:13.19 AFV.select :carrierSolution @06:56:13.29 delay 2 seconds @06:56:15.32 AS.reconfigure ASconfig @06:56:15.36 AS.seek 0.5ml @06:56:25.65 delay 1 second @06:56:26.67 AFV.select :holdingCoil @06:56:26.77 ASV.dial :waste1 @06:56:27.15 ATV.dial :air3 @06:56:28.43 AS.reconfigure ASslow1 @06:56:28.47 AS.seek 0.51ml @06:56:30.77 delay 2 seconds @06:56:32.79 ATV.dial :bleach @06:56:33.40 AS.seek 0.81ml @06:57:33.98 delay 3 seconds @06:57:37.00 ATV.dial :air3 @06:57:37.55 delay 3 seconds @06:57:40.62 AS.seek 0.835ml @06:57:45.98 delay 3 seconds @06:57:49.00 ATV.dial :bleach @06:57:49.60 AS.seek 0.82ml @06:57:52.88 delay 3 seconds @06:57:55.90 ARV.dial :waste @06:57:56.51 delay 3 seconds @06:57:59.53 ATV.dial :ARV @06:58:00.51 AS.reconfigure ASconfig @06:58:00.55 AS.seek 0.47ml @06:58:20.96 AFV.select :carrierSolution @06:58:21.06 delay 2 seconds @06:58:23.09 AS.seek 1ml @06:58:53.87 delay 1 second @06:58:54.89 AFV.select :holdingCoil @06:58:54.99 delay 1 second @06:58:56.02 AS.reconfigure ASfast @06:58:56.06 AS.seek 0ml @06:59:23.52 AFV.select :carrierSolution @06:59:23.62 delay 2 seconds @06:59:25.65 AS.reconfigure ASconfig @06:59:25.69 AS.seek 1ml @07:00:23.48 delay 1 second @07:00:24.50 AFV.select :holdingCoil @07:00:24.60 delay 1 second @07:00:25.63 AS.reconfigure ASfast @07:00:25.67 AS.seek 0ml @07:00:53.01 AFV.select :carrierSolution @07:00:53.11 delay 2 seconds @07:00:55.14 AS.reconfigure ASconfig @07:00:55.18 AS.seek 1ml @07:01:53.01 delay 1 second @07:01:54.03 AFV.select :holdingCoil @07:01:54.13 delay 1 second @07:01:55.16 AS.reconfigure ASfast @07:01:55.20 AS.seek 0ml @07:02:22.56 ARV.dial between carrier and air1 @07:02:23.04 ATV.dial :air3 @07:02:24.30 AS.reconfigure ASslow1 @07:02:24.34 AS.seek 0.01ml @07:02:26.70 delay 2 seconds @07:02:28.72 ATV.dial :bleach @07:02:29.81 AS.seek 0.81ml @07:05:10.93 delay 3 seconds @07:05:13.95 ATV.dial :air3 @07:05:14.49 delay 3 seconds @07:05:17.56 AS.seek 0.835ml @07:05:22.85 delay 3 seconds @07:05:25.87 ATV.dial :bleach @07:05:26.48 AS.seek 0.82ml @07:05:29.79 delay 3 seconds @07:05:32.81 ATV.dial PCR @07:05:33.20 AS.reconfigure ASconfig @07:05:33.24 AS.seek :empty @07:06:20.70 PCR.program :bleachProfile @07:06:20.75 PCR program should complete in < 2:45 @07:06:20.76 PCR.run 10:00 @07:09:22.59 PCR.off @07:09:22.61 AFV.select :carrierSolution @07:09:22.71 delay 2 seconds @07:09:24.74 AS.seek 1ml @07:10:22.54 delay 1 second @07:10:23.56 AFV.select :holdingCoil @07:10:23.66 delay 1 second @07:10:24.69 AS.reconfigure ASfast @07:10:24.73 AS.seek 0ml @07:10:52.07 delay 3 seconds @07:10:55.09 AFV.select :carrierSolution @07:10:55.19 delay 2 seconds @07:10:57.22 AS.reconfigure ASconfig @07:10:57.27 AS.seek 1ml @07:11:55.06 delay 1 second @07:11:56.08 AFV.select :holdingCoil @07:11:56.18 delay 1 second @07:11:57.21 AS.reconfigure ASfast @07:11:57.25 AS.seek 0ml @07:12:24.60 delay 3 seconds @07:12:27.62 AFV.select :carrierSolution @07:12:27.72 delay 2 seconds @07:12:29.75 AS.reconfigure ASconfig @07:12:29.80 AS.seek 1ml @07:13:27.57 delay 1 second @07:13:28.59 AFV.select :holdingCoil @07:13:28.69 delay 1 second @07:13:29.72 AS.reconfigure ASfast @07:13:29.76 AS.seek 0ml @07:13:57.12 delay 3 seconds @07:14:00.14 AFV.select :carrierSolution @07:14:00.24 delay 2 seconds @07:14:02.27 AS.reconfigure ASconfig @07:14:02.32 AS.seek 1ml @07:15:00.12 delay 1 second @07:15:01.14 AFV.select :holdingCoil @07:15:01.24 delay 1 second @07:15:02.27 AS.reconfigure ASfast @07:15:02.31 AS.seek 0ml @07:15:29.67 delay 3 seconds @07:15:32.69 AFV.select :carrierSolution @07:15:32.79 delay 2 seconds @07:15:34.82 AS.reconfigure ASconfig @07:15:34.87 AS.seek 1ml @07:16:32.61 delay 1 second @07:16:33.63 AFV.select :holdingCoil @07:16:33.73 delay 1 second @07:16:34.76 AS.reconfigure ASnorm @07:16:34.80 AS.seek 0ml @07:17:25.52 Setting up PCR for Bb -- writing /var/log/esp/Bb18aug2907h.pcr @07:17:25.53 Building PCR Slug from ASV with MM3 mix, PP5 primer @07:17:25.54 Priming with carrier @07:17:25.55 ATV.dial ARV @07:17:26.15 ARV.dial :carrier @07:17:26.49 AS.seek 0.5ml @07:17:52.06 AFV.select :carrierSolution @07:17:52.16 delay 2 seconds @07:17:54.19 AS.reconfigure ASconfig @07:17:54.23 AS.seek 1ml @07:18:23.29 delay 1 second @07:18:24.31 AFV.select :holdingCoil @07:18:24.41 delay 1 second @07:18:25.43 ATV.dial :waste @07:18:27.09 AS.reconfigure ASnorm @07:18:27.14 AS.seek :empty @07:19:17.87 AFV.select :carrierSolution @07:19:17.97 delay 2 seconds @07:19:20.00 AS.reconfigure ASconfig @07:19:20.04 AS.seek 0.5ml @07:19:49.11 delay 1 second @07:19:50.13 AFV.select :holdingCoil @07:19:50.23 Building Slug @07:19:50.24 ATV.dial :ARV @07:19:51.87 delay 2 seconds @07:19:53.89 ARV.dial :air1 @07:19:54.29 delay 2 seconds @07:19:56.36 AS.reconfigure ASslow2 @07:19:56.40 AS.seek 0.506ml @07:20:02.73 delay 2 seconds @07:20:04.75 ARV.dial :MM3 @07:20:05.81 delay 2 seconds @07:20:07.83 Adding MM3 @07:20:07.89 AS.seek 0.524ml @07:20:26.12 delay 25 seconds @07:20:51.14 ARV.dial :PP5 @07:20:52.03 Adding PP5 @07:20:52.04 delay 2 seconds @07:20:54.11 AS.seek 0.53ml @07:21:00.56 delay 10 seconds @07:21:10.59 ARV.dial :air3 @07:21:10.97 adding ARV air @07:21:10.98 delay 2 seconds @07:21:13.05 AS.seek 0.536ml @07:21:19.29 delay 5 seconds @07:21:24.31 ARV.dial :carrier @07:21:25.59 delay 7 seconds @07:21:32.67 AS.seek 0.594ml @07:22:31.31 delay 5 seconds @07:22:36.33 ATV.dial :ASV @07:22:36.73 delay 2 seconds @07:22:38.80 AS.seek 0.6ml @07:22:45.09 delay 4 seconds @07:22:49.11 ATV.dial ARV @07:22:49.65 delay 2 seconds @07:22:51.67 ARV.dial :carrier @07:22:51.68 delay 2 seconds @07:22:53.75 AS.seek 0.65ml @07:23:44.26 delay 4 seconds @07:23:48.28 ATV.dial PCR @07:23:48.89 ASV.dial :waste1 @07:23:48.90 ARV.dial :air1 @07:23:49.30 Positioning Slug by 0.324ml @07:23:49.38 AS.seek 0.326ml @07:29:15.19 delay 5 seconds @07:29:20.21 ATV.dial ARV @07:29:20.98 ASV.dial :PCRlock @07:29:21.36 delay 5 seconds @07:29:26.38 Running Bb Profile60C300 thermocycling @07:29:26.39 PCR.program Profile60C300 @07:29:26.51 Profile60C300 should complete in < 1:45:21 @07:29:26.52 PCR.run 2:16:41.25 @08:50:04.44 PCR.off @08:50:05.97 PCR for Bb completed -- closed /var/log/esp/Bb18aug2907h.pcr @08:50:06.03 Cleaning PCR @08:50:06.04 AFV.select :carrierSolution @08:50:06.13 delay 2 seconds @08:50:08.16 AS.reconfigure ASconfig @08:50:08.20 AS.seek 0.5ml @08:50:18.50 delay 1 second @08:50:19.52 AFV.select :holdingCoil @08:50:19.62 ASV.dial :waste1 @08:50:20.00 ATV.dial :air3 @08:50:21.28 AS.reconfigure ASslow1 @08:50:21.32 AS.seek 0.51ml @08:50:23.60 delay 2 seconds @08:50:25.62 ATV.dial :bleach @08:50:26.23 AS.seek 0.81ml @08:51:26.81 delay 3 seconds @08:51:29.83 ATV.dial :air3 @08:51:30.38 delay 3 seconds @08:51:33.45 AS.seek 0.835ml @08:51:38.74 delay 3 seconds @08:51:41.76 ATV.dial :bleach @08:51:42.37 AS.seek 0.82ml @08:51:45.69 delay 3 seconds @08:51:48.71 ARV.dial :waste @08:51:49.31 delay 3 seconds @08:51:52.33 ATV.dial :ARV @08:51:53.22 AS.reconfigure ASconfig @08:51:53.26 AS.seek 0.47ml @08:52:13.64 AFV.select :carrierSolution @08:52:13.74 delay 2 seconds @08:52:15.77 AS.seek 1ml @08:52:46.55 delay 1 second @08:52:47.57 AFV.select :holdingCoil @08:52:47.68 delay 1 second @08:52:48.71 AS.reconfigure ASfast @08:52:48.75 AS.seek 0ml @08:53:16.10 AFV.select :carrierSolution @08:53:16.20 delay 2 seconds @08:53:18.23 AS.reconfigure ASconfig @08:53:18.27 AS.seek 1ml @08:54:16.10 delay 1 second @08:54:17.12 AFV.select :holdingCoil @08:54:17.22 delay 1 second @08:54:18.25 AS.reconfigure ASfast @08:54:18.29 AS.seek 0ml @08:54:45.66 AFV.select :carrierSolution @08:54:45.76 delay 2 seconds @08:54:47.79 AS.reconfigure ASconfig @08:54:47.83 AS.seek 1ml @08:55:45.56 delay 1 second @08:55:46.58 AFV.select :holdingCoil @08:55:46.68 delay 1 second @08:55:47.71 AS.reconfigure ASfast @08:55:47.75 AS.seek 0ml @08:56:15.10 ARV.dial between carrier and air1 @08:56:15.58 ATV.dial :air3 @08:56:16.86 AS.reconfigure ASslow1 @08:56:16.90 AS.seek 0.01ml @08:56:19.19 delay 2 seconds @08:56:21.21 ATV.dial :bleach @08:56:21.81 AS.seek 0.81ml @08:59:02.96 delay 3 seconds @08:59:05.98 ATV.dial :air3 @08:59:06.53 delay 3 seconds @08:59:09.60 AS.seek 0.835ml @08:59:14.89 delay 3 seconds @08:59:17.91 ATV.dial :bleach @08:59:18.52 AS.seek 0.82ml @08:59:21.81 delay 3 seconds @08:59:24.83 ATV.dial PCR @08:59:25.22 AS.reconfigure ASconfig @08:59:25.27 AS.seek :empty @09:00:12.83 PCR.program :bleachProfile @09:00:12.87 PCR program should complete in < 2:45 @09:00:12.88 PCR.run 10:00 @09:03:15.19 PCR.off @09:03:15.22 AFV.select :carrierSolution @09:03:15.32 delay 2 seconds @09:03:17.35 AS.seek 1ml @09:04:15.17 delay 1 second @09:04:16.19 AFV.select :holdingCoil @09:04:16.29 delay 1 second @09:04:17.32 AS.reconfigure ASfast @09:04:17.36 AS.seek 0ml @09:04:45.02 delay 3 seconds @09:04:48.04 AFV.select :carrierSolution @09:04:48.14 delay 2 seconds @09:04:50.17 AS.reconfigure ASconfig @09:04:50.22 AS.seek 1ml @09:05:47.99 delay 1 second @09:05:49.01 AFV.select :holdingCoil @09:05:49.11 delay 1 second @09:05:50.14 AS.reconfigure ASfast @09:05:50.18 AS.seek 0ml @09:06:17.60 delay 3 seconds @09:06:20.62 AFV.select :carrierSolution @09:06:20.72 delay 2 seconds @09:06:22.75 AS.reconfigure ASconfig @09:06:22.79 AS.seek 1ml @09:07:20.57 delay 1 second @09:07:21.59 AFV.select :holdingCoil @09:07:21.69 delay 1 second @09:07:22.72 AS.reconfigure ASfast @09:07:22.76 AS.seek 0ml @09:07:50.11 delay 3 seconds @09:07:53.13 AFV.select :carrierSolution @09:07:53.23 delay 2 seconds @09:07:55.26 AS.reconfigure ASconfig @09:07:55.31 AS.seek 1ml @09:08:53.05 delay 1 second @09:08:54.07 AFV.select :holdingCoil @09:08:54.17 delay 1 second @09:08:55.20 AS.reconfigure ASfast @09:08:55.24 AS.seek 0ml @09:09:22.75 delay 3 seconds @09:09:25.77 AFV.select :carrierSolution @09:09:25.87 delay 2 seconds @09:09:27.90 AS.reconfigure ASconfig @09:09:27.94 AS.seek 1ml @09:10:25.72 delay 1 second @09:10:26.74 AFV.select :holdingCoil @09:10:26.84 delay 1 second @09:10:27.87 AS.reconfigure ASnorm @09:10:27.91 AS.seek 0ml @09:11:18.63 Setting up PCR for Tt2 -- writing /var/log/esp/Tt218aug2909h.pcr @09:11:18.64 Building PCR Slug from ASV with MM3 mix, PP6 primer @09:11:18.65 Priming with carrier @09:11:18.66 ATV.dial ARV @09:11:19.27 ARV.dial :carrier @09:11:19.61 AS.seek 0.5ml @09:11:45.13 AFV.select :carrierSolution @09:11:45.23 delay 2 seconds @09:11:47.26 AS.reconfigure ASconfig @09:11:47.30 AS.seek 1ml @09:12:16.30 delay 1 second @09:12:17.32 AFV.select :holdingCoil @09:12:17.42 delay 1 second @09:12:18.44 ATV.dial :waste @09:12:20.10 AS.reconfigure ASnorm @09:12:20.15 AS.seek :empty @09:13:10.86 AFV.select :carrierSolution @09:13:10.96 delay 2 seconds @09:13:12.99 AS.reconfigure ASconfig @09:13:13.03 AS.seek 0.5ml @09:13:42.11 delay 1 second @09:13:43.13 AFV.select :holdingCoil @09:13:43.23 Building Slug @09:13:43.24 ATV.dial :ARV @09:13:44.86 delay 2 seconds @09:13:46.88 ARV.dial :air1 @09:13:47.28 delay 2 seconds @09:13:49.35 AS.reconfigure ASslow2 @09:13:49.39 AS.seek 0.506ml @09:13:55.71 delay 2 seconds @09:13:57.73 ARV.dial :MM3 @09:13:58.77 delay 2 seconds @09:14:00.79 Adding MM3 @09:14:00.86 AS.seek 0.524ml @09:14:19.18 delay 25 seconds @09:14:44.20 ARV.dial :PP6 @09:14:44.57 Adding PP6 @09:14:44.58 delay 2 seconds @09:14:46.65 AS.seek 0.53ml @09:14:52.83 delay 10 seconds @09:15:02.86 ARV.dial :air1 @09:15:03.69 adding ARV air @09:15:03.70 delay 2 seconds @09:15:05.77 AS.seek 0.536ml @09:15:11.99 delay 5 seconds @09:15:17.01 ARV.dial :carrier @09:15:17.41 delay 7 seconds @09:15:24.49 AS.seek 0.594ml @09:16:22.94 delay 5 seconds @09:16:27.96 ATV.dial :ASV @09:16:28.37 delay 2 seconds @09:16:30.44 AS.seek 0.6ml @09:16:37.18 delay 4 seconds @09:16:41.20 ATV.dial ARV @09:16:41.74 delay 2 seconds @09:16:43.76 ARV.dial :carrier @09:16:43.77 delay 2 seconds @09:16:45.84 AS.seek 0.65ml @09:17:36.26 delay 4 seconds @09:17:40.28 ATV.dial PCR @09:17:40.90 ASV.dial :waste1 @09:17:40.91 ARV.dial :air1 @09:17:41.32 Positioning Slug by 0.324ml @09:17:41.39 AS.seek 0.326ml @09:23:07.40 delay 5 seconds @09:23:12.42 ATV.dial ARV @09:23:13.20 ASV.dial :PCRlock @09:23:13.57 delay 5 seconds @09:23:18.59 Running Tt2 Profile60C300 thermocycling @09:23:18.61 PCR.program Profile60C300 @09:23:18.72 Profile60C300 should complete in < 1:45:21 @09:23:18.73 PCR.run 2:16:41.25 @09:36:33.58 Session Begun @09:36:37.13 -> showlog 100 @09:37:02.11 -> exit @09:37:02.12 Session Ended @10:43:52.77 PCR.off @10:43:54.80 PCR for Tt2 completed -- closed /var/log/esp/Tt218aug2909h.pcr @10:43:54.86 Cleaning PCR @10:43:54.87 AFV.select :carrierSolution @10:43:54.98 delay 2 seconds @10:43:57.01 AS.reconfigure ASconfig @10:43:57.05 AS.seek 0.5ml @10:44:07.37 delay 1 second @10:44:08.39 AFV.select :holdingCoil @10:44:08.49 ASV.dial :waste1 @10:44:08.87 ATV.dial :air3 @10:44:10.15 AS.reconfigure ASslow1 @10:44:10.19 AS.seek 0.51ml @10:44:12.47 delay 2 seconds @10:44:14.49 ATV.dial :bleach @10:44:15.11 AS.seek 0.81ml @10:45:15.68 delay 3 seconds @10:45:18.70 ATV.dial :air3 @10:45:19.24 delay 3 seconds @10:45:22.31 AS.seek 0.835ml @10:45:27.60 delay 3 seconds @10:45:30.62 ATV.dial :bleach @10:45:31.23 AS.seek 0.82ml @10:45:34.48 delay 3 seconds @10:45:37.50 ARV.dial :waste @10:45:38.10 delay 3 seconds @10:45:41.12 ATV.dial :ARV @10:45:42.01 AS.reconfigure ASconfig @10:45:42.05 AS.seek 0.47ml @10:46:02.45 AFV.select :carrierSolution @10:46:02.55 delay 2 seconds @10:46:04.58 AS.seek 1ml @10:46:35.35 delay 1 second @10:46:36.37 AFV.select :holdingCoil @10:46:36.47 delay 1 second @10:46:37.50 AS.reconfigure ASfast @10:46:37.54 AS.seek 0ml @10:47:05.01 AFV.select :carrierSolution @10:47:05.11 delay 2 seconds @10:47:07.14 AS.reconfigure ASconfig @10:47:07.18 AS.seek 1ml @10:48:05.01 delay 1 second @10:48:06.03 AFV.select :holdingCoil @10:48:06.13 delay 1 second @10:48:07.16 AS.reconfigure ASfast @10:48:07.20 AS.seek 0ml @10:48:34.56 AFV.select :carrierSolution @10:48:34.66 delay 2 seconds @10:48:36.69 AS.reconfigure ASconfig @10:48:36.73 AS.seek 1ml @10:49:34.46 delay 1 second @10:49:35.48 AFV.select :holdingCoil @10:49:35.58 delay 1 second @10:49:36.61 AS.reconfigure ASfast @10:49:36.65 AS.seek 0ml @10:50:03.99 ARV.dial between carrier and air1 @10:50:04.48 ATV.dial :air3 @10:50:05.76 AS.reconfigure ASslow1 @10:50:05.80 AS.seek 0.01ml @10:50:08.12 delay 2 seconds @10:50:10.14 ATV.dial :bleach @10:50:10.74 AS.seek 0.81ml @10:52:51.96 delay 3 seconds @10:52:54.98 ATV.dial :air3 @10:52:55.53 delay 3 seconds @10:52:58.60 AS.seek 0.835ml @10:53:03.96 delay 3 seconds @10:53:06.98 ATV.dial :bleach @10:53:07.59 AS.seek 0.82ml @10:53:10.87 delay 3 seconds @10:53:13.89 ATV.dial PCR @10:53:14.28 AS.reconfigure ASconfig @10:53:14.32 AS.seek :empty @10:54:01.79 PCR.program :bleachProfile @10:54:01.84 PCR program should complete in < 2:45 @10:54:01.85 PCR.run 10:00 @10:57:03.67 PCR.off @10:57:03.69 AFV.select :carrierSolution @10:57:03.79 delay 2 seconds @10:57:05.82 AS.seek 1ml @10:58:03.61 delay 1 second @10:58:04.63 AFV.select :holdingCoil @10:58:04.73 delay 1 second @10:58:05.76 AS.reconfigure ASfast @10:58:05.80 AS.seek 0ml @10:58:33.18 delay 3 seconds @10:58:36.20 AFV.select :carrierSolution @10:58:36.79 delay 2 seconds @10:58:38.82 AS.reconfigure ASconfig @10:58:38.86 AS.seek 1ml @10:59:36.65 delay 1 second @10:59:37.67 AFV.select :holdingCoil @10:59:37.77 delay 1 second @10:59:38.80 AS.reconfigure ASfast @10:59:38.84 AS.seek 0ml @11:00:06.18 delay 3 seconds @11:00:09.20 AFV.select :carrierSolution @11:00:09.30 delay 2 seconds @11:00:11.33 AS.reconfigure ASconfig @11:00:11.38 AS.seek 1ml @11:01:09.17 delay 1 second @11:01:10.19 AFV.select :holdingCoil @11:01:10.29 delay 1 second @11:01:11.32 AS.reconfigure ASfast @11:01:11.36 AS.seek 0ml @11:01:38.70 delay 3 seconds @11:01:41.72 AFV.select :carrierSolution @11:01:41.82 delay 2 seconds @11:01:43.85 AS.reconfigure ASconfig @11:01:43.89 AS.seek 1ml @11:02:41.65 delay 1 second @11:02:42.67 AFV.select :holdingCoil @11:02:42.77 delay 1 second @11:02:43.80 AS.reconfigure ASfast @11:02:43.84 AS.seek 0ml @11:03:11.26 delay 3 seconds @11:03:14.28 AFV.select :carrierSolution @11:03:14.38 delay 2 seconds @11:03:16.41 AS.reconfigure ASconfig @11:03:16.46 AS.seek 1ml @11:04:14.23 delay 1 second @11:04:15.25 AFV.select :holdingCoil @11:04:15.35 delay 1 second @11:04:16.38 AS.reconfigure ASnorm @11:04:16.42 AS.seek 0ml @11:05:07.13 Setting up PCR for IPc -- writing /var/log/esp/IPc18aug2911h.pcr @11:05:07.14 Building PCR Slug from ASV with MM1 mix, PP1 primer @11:05:07.15 Priming with carrier @11:05:07.16 ATV.dial ARV @11:05:07.78 ARV.dial :carrier @11:05:08.12 AS.seek 0.5ml @11:05:33.69 AFV.select :carrierSolution @11:05:33.79 delay 2 seconds @11:05:35.82 AS.reconfigure ASconfig @11:05:35.86 AS.seek 1ml @11:06:04.94 delay 1 second @11:06:05.96 AFV.select :holdingCoil @11:06:06.06 delay 1 second @11:06:07.08 ATV.dial :waste @11:06:08.73 AS.reconfigure ASnorm @11:06:08.78 AS.seek :empty @11:06:59.51 AFV.select :carrierSolution @11:06:59.61 delay 2 seconds @11:07:01.64 AS.reconfigure ASconfig @11:07:01.68 AS.seek 0.5ml @11:07:30.76 delay 1 second @11:07:31.78 AFV.select :holdingCoil @11:07:31.88 Building Slug @11:07:31.89 ATV.dial :ARV @11:07:33.51 delay 2 seconds @11:07:35.53 ARV.dial :air1 @11:07:35.92 delay 2 seconds @11:07:37.99 AS.reconfigure ASslow2 @11:07:38.04 AS.seek 0.506ml @11:07:44.22 delay 2 seconds @11:07:46.24 ARV.dial :MM1 @11:07:46.83 delay 2 seconds @11:07:48.85 Adding MM1 @11:07:48.91 AS.seek 0.524ml @11:08:07.20 delay 25 seconds @11:08:32.22 ARV.dial :PP1 @11:08:32.61 Adding PP1 @11:08:32.62 delay 2 seconds @11:08:34.69 AS.seek 0.53ml @11:08:40.95 delay 10 seconds @11:08:50.98 ARV.dial :air1 @11:08:51.36 adding ARV air @11:08:51.37 delay 2 seconds @11:08:53.44 AS.seek 0.536ml @11:08:59.91 delay 5 seconds @11:09:04.93 ARV.dial :carrier @11:09:05.33 delay 7 seconds @11:09:12.41 AS.seek 0.594ml @11:10:11.06 delay 5 seconds @11:10:16.08 ATV.dial :ASV @11:10:16.48 delay 2 seconds @11:10:18.55 AS.seek 0.6ml @11:10:24.83 delay 4 seconds @11:10:28.85 ATV.dial ARV @11:10:29.41 delay 2 seconds @11:10:31.43 ARV.dial :carrier @11:10:31.44 delay 2 seconds @11:10:33.51 AS.seek 0.65ml @11:11:24.20 delay 4 seconds @11:11:28.22 ATV.dial PCR @11:11:28.83 ASV.dial :waste1 @11:11:28.84 ARV.dial :air1 @11:11:29.24 Positioning Slug by 0.324ml @11:11:29.32 AS.seek 0.326ml @11:16:55.64 delay 5 seconds @11:17:00.66 ATV.dial ARV @11:17:01.94 ASV.dial :PCRlock @11:17:02.31 delay 5 seconds @11:17:07.33 Running IPc ProfileIPc thermocycling @11:17:07.35 PCR.program ProfileIPc @11:17:07.48 ProfileIPc should complete in < 1:50:49 @11:17:07.49 PCR.run 2:23:31.25 @11:47:26.13 Session Begun @11:47:29.79 -> showlog 100 @21:11:52.55CEST29-Aug-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @21:11:54.28 Copyright 2017 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @21:11:54.54 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @21:11:58.87 Polling Can every 10:00 @21:12:01.14 SPE.coast @21:12:01.23 SPE.configure SPEconfig @21:12:01.39 AS.reconfigure ASconfig @21:12:01.47 AS.hold @21:12:01.56 AS.configure ASconfig @21:12:01.72 SS.reconfigure SSconfig @21:12:01.80 SS.hold @21:12:01.89 SS.configure SSconfig @21:12:02.06 CC.reconfigure CCconfig @21:12:02.12 CC.hold @21:12:02.22 CC.configure CCconfig @21:12:02.38 CS.reconfigure CSconfig @21:12:02.46 CS.hold @21:12:02.56 CS.configure CSconfig @21:12:03.39 PC.reconfigure PCconfig @21:12:03.46 PC.hold @21:12:03.56 PC.configure PCconfig @21:12:03.72 PS.configure PSconfig @21:12:03.90 Forearm.reconfigure LooseForearm @21:12:03.99 Forearm.hold @21:12:04.09 Forearm.configure LooseForearm @21:12:04.23 Elbow.reconfigure LooseElbow @21:12:04.27 Elbow.hold @21:12:04.31 Elbow.configure LooseElbow @21:12:04.41 SC.reconfigure SCloose @21:12:04.45 SC.hold @21:12:04.51 SC.configure SCloose @21:12:04.59 SE.reconfigure SEconfig @21:12:04.63 SE.hold @21:12:04.68 SE.configure SEconfig @21:12:04.72 CH.coast @21:12:04.76 CH.configure CHconfig @21:12:04.79 PH.coast @21:12:04.85 PH.configure PHconfig @21:12:04.88 PCR.off @21:12:04.91 PCR.pidtemp= 50,0.1,0,300,25,25 @21:12:04.93 PCR.caltemp= 2.7073,0.0373,0 @21:12:04.96 All dwarf controllers running firmware version 3.75 @21:12:05.08 Cleanly exited at 21:11:22CEST29-Aug-18 @21:12:05.11 Loading /home/esp/esp2/mission/decontest.rb mission @21:12:05.63 deconEluateRecoveryLine extSrc ATV.dial :waste @21:12:06.88 AS.reconfigure ASnorm @21:12:06.92 AS.seek 0ml @21:12:06.97 ATV.dial :air3 @21:12:07.91 AS.reconfigure ASslow1 @21:12:07.95 AS.seek 0.01ml @21:12:10.30 delay 2 seconds @21:12:12.32 ATV.dial :bleach @21:12:12.78 AS.seek 0.31ml @21:12:16.30 Session Begun @21:12:20.85 -> showlog 100 @21:12:30.61 -> exit @21:12:30.62 Session Ended @21:13:13.43
delay 3 seconds @21:13:16.45 ATV.dial :air3 @21:13:17.01 delay 3 seconds @21:13:20.09 AS.seek 0.335ml @21:13:25.39 delay 3 seconds @21:13:28.41 ATV.dial :bleach @21:13:29.02 AS.seek 0.32ml @21:13:32.32 delay 3 seconds @21:13:35.34 ATV.dial :extSrc @21:13:35.95 AS.seek 0ml @21:14:40.57 AFV.select :carrierSolution @21:14:40.68 delay 2 seconds @21:14:42.70 AS.reconfigure ASconfig @21:14:42.75 AS.seek 0.5ml @21:15:11.74 delay 1 second @21:15:12.76 AFV.select :holdingCoil @21:15:12.86 ATV.dial :extSrc @21:15:12.88 AS.reconfigure ASslow1 @21:15:12.93 AS.seek 0ml @21:16:53.73 AFV.select :carrierSolution @21:16:53.84 delay 2 seconds @21:16:55.86 AS.reconfigure ASconfig @21:16:55.91 AS.seek 0.5ml @21:17:24.91 delay 1 second @21:17:25.93 AFV.select :holdingCoil @21:17:26.03 ATV.dial :extSrc @21:17:26.05 AS.reconfigure ASslow1 @21:17:26.10 AS.seek 0ml @21:19:06.90 AFV.select :carrierSolution @21:19:07.00 delay 2 seconds @21:19:09.02 AS.reconfigure ASconfig @21:19:09.07 AS.seek 0.5ml @21:19:38.10 delay 1 second @21:19:39.11 AFV.select :holdingCoil @21:19:39.21 ATV.dial :extSrc @21:19:39.23 AS.reconfigure ASslow1 @21:19:39.28 AS.seek 0ml @21:21:20.10 AFV.select :carrierSolution @21:21:20.21 delay 2 seconds @21:21:22.23 AS.reconfigure ASconfig @21:21:22.28 AS.seek 0.5ml @21:21:51.30 delay 1 second @21:21:52.31 AFV.select :holdingCoil @21:21:52.41 ATV.dial :extSrc @21:21:52.43 AS.reconfigure ASslow1 @21:21:52.48 AS.seek 0ml @21:23:33.32 ATV.dial :air2 @21:23:34.65 AS.reconfigure ASslow0 @21:23:34.69 AS.seek 0.5ml @21:24:25.24 ATV.dial :extSrc @21:24:26.71 AS.seek 0.02ml @21:25:15.29 delay 1 second @21:25:16.31 ATV.dial :waste @21:25:17.60 AS.reconfigure ASconfig @21:25:17.64 AS.seek :empty @21:25:19.13 delay 20 seconds @21:25:39.15 deconEluateRecoveryLine extSrc @21:25:39.16 ATV.dial :waste @21:25:39.18 AS.reconfigure ASnorm @21:25:39.23 AS.seek 0ml @21:25:39.59 ATV.dial :air3 @21:25:40.41 AS.reconfigure ASslow1 @21:25:40.45 AS.seek 0.01ml @21:25:42.73 delay 2 seconds @21:25:44.75 ATV.dial :bleach @21:25:45.21 AS.seek 0.31ml @21:26:45.82 delay 3 seconds @21:26:48.84 ATV.dial :air3 @21:26:49.38 delay 3 seconds @21:26:52.45 AS.seek 0.335ml @21:26:57.75 delay 3 seconds @21:27:00.77 ATV.dial :bleach @21:27:01.38 AS.seek 0.32ml @21:27:04.66 delay 3 seconds @21:27:07.68 ATV.dial :extSrc @21:27:08.30 AS.seek 0ml @21:28:12.85 AFV.select :carrierSolution @21:28:12.95 delay 2 seconds @21:28:14.97 AS.reconfigure ASconfig @21:28:15.02 AS.seek 0.5ml @21:28:44.07 delay 1 second @21:28:45.08 AFV.select :holdingCoil @21:28:45.18 ATV.dial :extSrc @21:28:45.20 AS.reconfigure ASslow1 @21:28:45.25 AS.seek 0ml @21:30:26.07 AFV.select :carrierSolution @21:30:26.18 delay 2 seconds @21:30:28.20 AS.reconfigure ASconfig @21:30:28.25 AS.seek 0.5ml @21:30:57.27 delay 1 second @21:30:58.28 AFV.select :holdingCoil @21:30:58.38 ATV.dial :extSrc @21:30:58.40 AS.reconfigure ASslow1 @21:30:58.45 AS.seek 0ml @21:32:39.28 AFV.select :carrierSolution @21:32:39.39 delay 2 seconds @21:32:41.41 AS.reconfigure ASconfig @21:32:41.46 AS.seek 0.5ml @21:33:10.45 delay 1 second @21:33:11.47 AFV.select :holdingCoil @21:33:11.57 ATV.dial :extSrc @21:33:11.59 AS.reconfigure ASslow1 @21:33:11.64 AS.seek 0ml @21:34:52.48 AFV.select :carrierSolution @21:34:52.59 delay 2 seconds @21:34:54.61 AS.reconfigure ASconfig @21:34:54.66 AS.seek 0.5ml @21:35:23.68 delay 1 second @21:35:24.70 AFV.select :holdingCoil @21:35:24.80 ATV.dial :extSrc @21:35:24.82 AS.reconfigure ASslow1 @21:35:24.87 AS.seek 0ml @21:37:05.70 ATV.dial :air2 @21:37:07.02 AS.reconfigure ASslow0 @21:37:07.06 AS.seek 0.5ml @21:37:57.62 ATV.dial :extSrc @21:37:59.47 AS.seek 0.02ml @21:38:48.01 delay 1 second @21:38:49.03 ATV.dial :waste @21:38:50.32 AS.reconfigure ASconfig @21:38:50.36 AS.seek :empty @21:38:51.82 delay 20 seconds @21:39:11.84 deconEluateRecoveryLine extSrc @21:39:11.85 ATV.dial :waste @21:39:11.87 AS.reconfigure ASnorm @21:39:11.92 AS.seek 0ml @21:39:12.23 ATV.dial :air3 @21:39:13.06 AS.reconfigure ASslow1 @21:39:13.10 AS.seek 0.01ml @21:39:15.39 delay 2 seconds @21:39:17.41 ATV.dial :bleach @21:39:17.85 AS.seek 0.31ml @21:40:18.46 delay 3 seconds @21:40:21.48 ATV.dial :air3 @21:40:22.03 delay 3 seconds @21:40:25.10 AS.seek 0.335ml @21:40:30.41 delay 3 seconds @21:40:33.43 ATV.dial :bleach @21:40:34.04 AS.seek 0.32ml @21:40:37.34 delay 3 seconds @21:40:40.36 ATV.dial :extSrc @21:40:40.98 AS.seek 0ml @21:41:45.61 AFV.select :carrierSolution @21:41:45.72 delay 2 seconds @21:41:47.74 AS.reconfigure ASconfig @21:41:47.79 AS.seek 0.5ml @21:42:16.81 delay 1 second @21:42:17.83 AFV.select :holdingCoil @21:42:17.93 ATV.dial :extSrc @21:42:17.95 AS.reconfigure ASslow1 @21:42:18.00 AS.seek 0ml @21:43:58.82 AFV.select :carrierSolution @21:43:58.93 delay 2 seconds @21:44:00.95 AS.reconfigure ASconfig @21:44:01.00 AS.seek 0.5ml @21:44:30.03 delay 1 second @21:44:31.04 AFV.select :holdingCoil @21:44:31.14 ATV.dial :extSrc @21:44:31.16 AS.reconfigure ASslow1 @21:44:31.21 AS.seek 0ml @21:46:12.01 AFV.select :carrierSolution @21:46:12.11 delay 2 seconds @21:46:14.13 AS.reconfigure ASconfig @21:46:14.18 AS.seek 0.5ml @21:46:43.20 delay 1 second @21:46:44.22 AFV.select :holdingCoil @21:46:44.31 ATV.dial :extSrc @21:46:44.33 AS.reconfigure ASslow1 @21:46:44.38 AS.seek 0ml @21:48:25.18 AFV.select :carrierSolution @21:48:25.28 delay 2 seconds @21:48:27.30 AS.reconfigure ASconfig @21:48:27.35 AS.seek 0.5ml @21:48:56.37 delay 1 second @21:48:57.39 AFV.select :holdingCoil @21:48:57.49 ATV.dial :extSrc @21:48:57.51 AS.reconfigure ASslow1 @21:48:57.56 AS.seek 0ml @21:50:38.40 ATV.dial :air2 @21:50:39.73 AS.reconfigure ASslow0 @21:50:39.77 AS.seek 0.5ml @21:51:30.40 ATV.dial :extSrc @21:51:31.85 AS.seek 0.02ml @21:52:20.42 delay 1 second @21:52:21.44 ATV.dial :waste @21:52:22.73 AS.reconfigure ASconfig @21:52:22.77 AS.seek :empty @21:52:24.26 delay 20 seconds @21:52:44.33 Cleaning PCR AFV.select :carrierSolution @21:52:44.44 delay 2 seconds @21:52:46.46 AS.seek 0.5ml @21:53:15.55 delay 1 second @21:53:16.56 AFV.select :holdingCoil @21:53:16.65 ASV.dial :waste1 @21:53:16.73 ATV.dial :air3 @21:53:17.56 AS.reconfigure ASslow1 @21:53:17.60 AS.seek 0.51ml @21:53:19.87 delay 2 seconds @21:53:21.89 ATV.dial :bleach @21:53:22.34 AS.seek 0.81ml @21:54:23.03 delay 3 seconds @21:54:26.05 ATV.dial :air3 @21:54:26.59 delay 3 seconds @21:54:29.66 AS.seek 0.835ml @21:54:34.98 delay 3 seconds @21:54:38.00 ATV.dial :bleach @21:54:38.61 AS.seek 0.82ml @21:54:41.92 delay 3 seconds @21:54:44.94 ARV.dial :waste @21:54:45.44 delay 3 seconds @21:54:48.46 ATV.dial :ARV @21:54:49.33 AS.reconfigure ASconfig @21:54:49.38 AS.seek 0.47ml @21:55:09.78 AFV.select :carrierSolution @21:55:09.89 delay 2 seconds @21:55:11.91 AS.seek 1ml @21:55:42.67 delay 1 second @21:55:43.68 AFV.select :holdingCoil @21:55:43.79 delay 1 second @21:55:44.81 AS.reconfigure ASfast @21:55:44.86 AS.seek 0ml @21:56:12.75 AFV.select :carrierSolution @21:56:12.86 delay 2 seconds @21:56:14.88 AS.reconfigure ASconfig @21:56:14.93 AS.seek 1ml @21:57:12.69 delay 1 second @21:57:13.70 AFV.select :holdingCoil @21:57:13.81 delay 1 second @21:57:14.83 AS.reconfigure ASfast @21:57:14.88 AS.seek 0ml @21:57:42.23 AFV.select :carrierSolution @21:57:42.34 delay 2 seconds @21:57:44.36 AS.reconfigure ASconfig @21:57:44.41 AS.seek 1ml @21:58:42.17 delay 1 second @21:58:43.18 AFV.select :holdingCoil @21:58:43.29 delay 1 second @21:58:44.31 AS.reconfigure ASfast @21:58:44.36 AS.seek 0ml @21:59:11.70 ARV.dial between carrier and air1 @21:59:12.20 ATV.dial :air3 @21:59:13.50 AS.reconfigure ASslow1 @21:59:13.54 AS.seek 0.01ml @21:59:15.87 delay 2 seconds @21:59:17.89 ATV.dial :bleach @21:59:18.51 AS.seek 0.81ml @22:01:59.62 delay 3 seconds @22:02:02.64 ATV.dial :air3 @22:02:03.19 delay 3 seconds @22:02:06.26 AS.seek 0.835ml @22:02:11.56 delay 3 seconds @22:02:14.58 ATV.dial :bleach @22:02:15.18 AS.seek 0.82ml @22:02:18.47 delay 3 seconds @22:02:21.49 ATV.dial PCR @22:02:21.87 AS.reconfigure ASconfig @22:02:21.91 AS.seek :empty @22:03:09.36 PCR.program :bleachProfile @22:03:09.40 PCR program should complete in < 2:45 @22:03:09.41 PCR.run 10:00 @22:06:14.66 PCR.off @22:06:14.69 AFV.select :carrierSolution @22:06:14.79 delay 2 seconds @22:06:16.81 AS.seek 1ml @22:07:14.64 delay 1 second @22:07:15.66 AFV.select :holdingCoil @22:07:15.77 delay 1 second @22:07:16.79 AS.reconfigure ASfast @22:07:16.84 AS.seek 0ml @22:07:44.19 delay 3 seconds @22:07:47.21 AFV.select :carrierSolution @22:07:47.32 delay 2 seconds @22:07:49.34 AS.reconfigure ASconfig @22:07:49.39 AS.seek 1ml @22:08:47.13 delay 1 second @22:08:48.15 AFV.select :holdingCoil @22:08:48.26 delay 1 second @22:08:49.28 AS.reconfigure ASfast @22:08:49.33 AS.seek 0ml @22:09:16.67 delay 3 seconds @22:09:19.69 AFV.select :carrierSolution @22:09:19.80 delay 2 seconds @22:09:21.82 AS.reconfigure ASconfig @22:09:21.88 AS.seek 1ml @22:10:19.67 delay 1 second @22:10:20.68 AFV.select :holdingCoil @22:10:20.79 delay 1 second @22:10:21.81 AS.reconfigure ASfast @22:10:21.86 AS.seek 0ml @22:10:49.22 delay 3 seconds @22:10:52.24 AFV.select :carrierSolution @22:10:52.35 delay 2 seconds @22:10:54.37 AS.reconfigure ASconfig @22:10:54.43 AS.seek 1ml @22:11:52.20 delay 1 second @22:11:53.22 AFV.select :holdingCoil @22:11:53.33 delay 1 second @22:11:54.35 AS.reconfigure ASfast @22:11:54.40 AS.seek 0ml @22:12:21.74 delay 3 seconds @22:12:24.76 AFV.select :carrierSolution @22:12:24.87 delay 2 seconds @22:12:26.89 AS.reconfigure ASconfig @22:12:26.95 AS.seek 1ml @22:13:24.72 delay 1 second @22:13:25.74 AFV.select :holdingCoil @22:13:25.85 delay 1 second @22:13:26.87 AS.reconfigure ASnorm @22:13:26.92 AS.seek 0ml @22:14:17.61 delay 20 seconds @22:14:37.68 Cleaning PCR AFV.select :carrierSolution @22:14:37.79 delay 2 seconds @22:14:39.81 AS.reconfigure ASconfig @22:14:39.86 AS.seek 0.5ml @22:15:08.88 delay 1 second @22:15:09.89 AFV.select :holdingCoil @22:15:09.99 ASV.dial :waste1 @22:15:10.00 ATV.dial :air3 @22:15:10.84 AS.reconfigure ASslow1 @22:15:10.88 AS.seek 0.51ml @22:15:13.16 delay 2 seconds @22:15:15.18 ATV.dial :bleach @22:15:15.78 AS.seek 0.81ml @22:16:16.39 delay 3 seconds @22:16:19.41 ATV.dial :air3 @22:16:19.95 delay 3 seconds @22:16:23.02 AS.seek 0.835ml @22:16:28.39 delay 3 seconds @22:16:31.41 ATV.dial :bleach @22:16:32.01 AS.seek 0.82ml @22:16:35.31 delay 3 seconds @22:16:38.33 ARV.dial :waste @22:16:38.83 delay 3 seconds @22:16:41.85 ATV.dial :ARV @22:16:42.73 AS.reconfigure ASconfig @22:16:42.77 AS.seek 0.47ml @22:17:03.17 AFV.select :carrierSolution @22:17:03.27 delay 2 seconds @22:17:05.29 AS.seek 1ml @22:17:36.05 delay 1 second @22:17:37.06 AFV.select :holdingCoil @22:17:37.16 delay 1 second @22:17:38.18 AS.reconfigure ASfast @22:17:38.23 AS.seek 0ml @22:18:05.56 AFV.select :carrierSolution @22:18:05.66 delay 2 seconds @22:18:07.68 AS.reconfigure ASconfig @22:18:07.73 AS.seek 1ml @22:19:05.50 delay 1 second @22:19:06.51 AFV.select :holdingCoil @22:19:06.62 delay 1 second @22:19:07.64 AS.reconfigure ASfast @22:19:07.69 AS.seek 0ml @22:19:35.03 AFV.select :carrierSolution @22:19:35.14 delay 2 seconds @22:19:37.16 AS.reconfigure ASconfig @22:19:37.21 AS.seek 1ml @22:20:34.99 delay 1 second @22:20:36.00 AFV.select :holdingCoil @22:20:36.10 delay 1 second @22:20:37.12 AS.reconfigure ASfast @22:20:37.17 AS.seek 0ml @22:21:04.52 ARV.dial between carrier and air1 @22:21:05.11 ATV.dial :air3 @22:21:06.39 AS.reconfigure ASslow1 @22:21:06.43 AS.seek 0.01ml @22:21:08.75 delay 2 seconds @22:21:10.77 ATV.dial :bleach @22:21:11.37 AS.seek 0.81ml @22:23:52.50 delay 3 seconds @22:23:55.52 ATV.dial :air3 @22:23:56.06 delay 3 seconds @22:23:59.13 AS.seek 0.835ml @22:24:04.40 delay 3 seconds @22:24:07.42 ATV.dial :bleach @22:24:08.03 AS.seek 0.82ml @22:24:11.28 delay 3 seconds @22:24:14.30 ATV.dial PCR @22:24:14.69 AS.reconfigure ASconfig @22:24:14.73 AS.seek :empty @22:25:02.20 PCR.program :bleachProfile @22:25:02.24 PCR program should complete in < 2:45 @22:25:02.25 PCR.run 10:00 @22:28:04.08 PCR.off @22:28:04.11 AFV.select :carrierSolution @22:28:04.21 delay 2 seconds @22:28:06.23 AS.seek 1ml @22:29:04.03 delay 1 second @22:29:05.04 AFV.select :holdingCoil @22:29:05.15 delay 1 second @22:29:06.17 AS.reconfigure ASfast @22:29:06.22 AS.seek 0ml @22:29:33.55 delay 3 seconds @22:29:36.57 AFV.select :carrierSolution @22:29:36.67 delay 2 seconds @22:29:38.69 AS.reconfigure ASconfig @22:29:38.74 AS.seek 1ml @22:30:36.47 delay 1 second @22:30:37.48 AFV.select :holdingCoil @22:30:37.59 delay 1 second @22:30:38.61 AS.reconfigure ASfast @22:30:38.66 AS.seek 0ml @22:31:06.14 delay 3 seconds @22:31:09.16 AFV.select :carrierSolution @22:31:09.26 delay 2 seconds @22:31:11.28 AS.reconfigure ASconfig @22:31:11.34 AS.seek 1ml @22:32:09.11 delay 1 second @22:32:10.13 AFV.select :holdingCoil @22:32:10.24 delay 1 second @22:32:11.26 AS.reconfigure ASfast @22:32:11.31 AS.seek 0ml @22:32:38.64 delay 3 seconds @22:32:41.66 AFV.select :carrierSolution @22:32:41.76 delay 2 seconds @22:32:43.78 AS.reconfigure ASconfig @22:32:43.83 AS.seek 1ml @22:33:41.56 delay 1 second @22:33:42.57 AFV.select :holdingCoil @22:33:42.68 delay 1 second @22:33:43.70 AS.reconfigure ASfast @22:33:43.75 AS.seek 0ml @22:34:11.53 delay 3 seconds @22:34:14.55 AFV.select :carrierSolution @22:34:14.66 delay 2 seconds @22:34:16.68 AS.reconfigure ASconfig @22:34:16.74 AS.seek 1ml @22:35:14.52 delay 1 second @22:35:15.54 AFV.select :holdingCoil @22:35:15.65 delay 1 second @22:35:16.67 AS.reconfigure ASnorm @22:35:16.72 AS.seek 0ml @22:36:07.41 delay 20 seconds @22:36:27.48 Cleaning PCR @22:36:27.49 AFV.select :carrierSolution @22:36:27.59 delay 2 seconds @22:36:29.61 AS.reconfigure ASconfig @22:36:29.66 AS.seek 0.5ml @22:36:58.72 delay 1 second @22:36:59.74 AFV.select :holdingCoil @22:36:59.84 ASV.dial :waste1 @22:36:59.86 ATV.dial :air3 @22:37:00.69 AS.reconfigure ASslow1 @22:37:00.73 AS.seek 0.51ml @22:37:03.00 delay 2 seconds @22:37:05.02 ATV.dial :bleach @22:37:05.63 AS.seek 0.81ml @22:38:06.21 delay 3 seconds @22:38:09.23 ATV.dial :air3 @22:38:09.78 delay 3 seconds @22:38:12.85 AS.seek 0.835ml @22:38:18.21 delay 3 seconds @22:38:21.23 ATV.dial :bleach @22:38:21.84 AS.seek 0.82ml @22:38:25.13 delay 3 seconds @22:38:28.15 ARV.dial :waste @22:38:28.63 delay 3 seconds @22:38:31.65 ATV.dial :ARV @22:38:32.53 AS.reconfigure ASconfig @22:38:32.57 AS.seek 0.47ml @22:38:52.97 AFV.select :carrierSolution @22:38:53.08 delay 2 seconds @22:38:55.10 AS.seek 1ml @22:39:25.88 delay 1 second @22:39:26.90 AFV.select :holdingCoil @22:39:27.01 delay 1 second @22:39:28.03 AS.reconfigure ASfast @22:39:28.08 AS.seek 0ml @22:39:55.41 AFV.select :carrierSolution @22:39:55.52 delay 2 seconds @22:39:57.54 AS.reconfigure ASconfig @22:39:57.59 AS.seek 1ml @22:40:55.32 delay 1 second @22:40:56.34 AFV.select :holdingCoil @22:40:56.45 delay 1 second @22:40:57.47 AS.reconfigure ASfast @22:40:57.52 AS.seek 0ml @22:41:24.85 AFV.select :carrierSolution @22:41:24.96 delay 2 seconds @22:41:26.98 AS.reconfigure ASconfig @22:41:27.03 AS.seek 1ml @22:42:24.80 delay 1 second @22:42:25.82 AFV.select :holdingCoil @22:42:25.93 delay 1 second @22:42:26.95 AS.reconfigure ASfast @22:42:27.00 AS.seek 0ml @22:42:54.35 ARV.dial between carrier and air1 @22:42:54.85 ATV.dial :air3 @22:42:56.14 AS.reconfigure ASslow1 @22:42:56.18 AS.seek 0.01ml @22:42:58.55 delay 2 seconds @22:43:00.57 ATV.dial :bleach @22:43:01.19 AS.seek 0.81ml @22:45:42.33 delay 3 seconds @22:45:45.35 ATV.dial :air3 @22:45:45.91 delay 3 seconds @22:45:48.98 AS.seek 0.835ml @22:45:54.27 delay 3 seconds @22:45:57.29 ATV.dial :bleach @22:45:57.91 AS.seek 0.82ml @22:46:01.21 delay 3 seconds @22:46:04.23 ATV.dial PCR @22:46:04.61 AS.reconfigure ASconfig @22:46:04.65 AS.seek :empty @22:46:52.11 PCR.program :bleachProfile @22:46:52.16 PCR program should complete in < 2:45 PCR.run 10:00 @22:49:57.01 PCR.off @22:49:57.04 AFV.select :carrierSolution @22:49:57.15 delay 2 seconds @22:49:59.17 AS.seek 1ml @22:50:56.97 delay 1 second @22:50:57.99 AFV.select :holdingCoil @22:50:58.10 delay 1 second @22:50:59.12 AS.reconfigure ASfast @22:50:59.17 AS.seek 0ml @22:51:26.57 delay 3 seconds @22:51:29.59 AFV.select :carrierSolution @22:51:29.70 delay 2 seconds @22:51:31.72 AS.reconfigure ASconfig @22:51:31.78 AS.seek 1ml @22:52:29.52 delay 1 second @22:52:30.53 AFV.select :holdingCoil @22:52:30.63 delay 1 second @22:52:31.65 AS.reconfigure ASfast @22:52:31.70 AS.seek 0ml @22:52:59.02 delay 3 seconds @22:53:02.04 AFV.select :carrierSolution @22:53:02.15 delay 2 seconds @22:53:04.17 AS.reconfigure ASconfig @22:53:04.22 AS.seek 1ml @22:54:01.99 delay 1 second @22:54:03.01 AFV.select :holdingCoil @22:54:03.12 delay 1 second @22:54:04.14 AS.reconfigure ASfast @22:54:04.19 AS.seek 0ml @22:54:31.71 delay 3 seconds @22:54:34.73 AFV.select :carrierSolution @22:54:35.07 delay 2 seconds @22:54:37.16 AS.reconfigure ASconfig @22:54:37.21 AS.seek 1ml @22:55:34.99 delay 1 second @22:55:36.01 AFV.select :holdingCoil @22:55:36.12 delay 1 second @22:55:37.14 AS.reconfigure ASfast @22:55:37.19 AS.seek 0ml @22:56:04.52 delay 3 seconds @22:56:07.54 AFV.select :carrierSolution @22:56:07.65 delay 2 seconds @22:56:09.67 AS.reconfigure ASconfig @22:56:09.72 AS.seek 1ml @22:57:07.49 delay 1 second @22:57:08.51 AFV.select :holdingCoil @22:57:08.62 delay 1 second @22:57:09.64 AS.reconfigure ASnorm @22:57:09.69 AS.seek 0ml @22:58:00.37 delay 20 seconds @22:58:20.52 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118aug2922h.pcr @22:58:20.54 Building PCR Slug from elutionWater with MM2 mix, PP4 primer @22:58:20.55 Priming with carrier @22:58:20.56 ATV.dial ARV @22:58:21.16 ARV.dial :carrier @22:58:21.50 AS.seek 0.5ml @22:58:47.05 AFV.select :carrierSolution @22:58:47.16 delay 2 seconds @22:58:49.18 AS.reconfigure ASconfig @22:58:49.23 AS.seek 1ml @22:59:18.24 delay 1 second @22:59:19.26 AFV.select :holdingCoil @22:59:19.37 delay 1 second @22:59:20.38 ATV.dial :waste @22:59:22.05 AS.reconfigure ASnorm @22:59:22.10 AS.seek :empty @23:00:12.84 AFV.select :carrierSolution @23:00:12.95 delay 2 seconds @23:00:14.97 AS.reconfigure ASconfig @23:00:15.02 AS.seek 0.5ml @23:00:44.13 delay 1 second @23:00:45.15 AFV.select :holdingCoil @23:00:45.26 Building Slug @23:00:45.27 ATV.dial :ARV @23:00:46.90 delay 2 seconds @23:00:48.92 ARV.dial :air1 @23:00:49.31 delay 2 seconds @23:00:51.38 AS.reconfigure ASslow2 @23:00:51.43 AS.seek 0.506ml @23:00:57.69 delay 2 seconds @23:00:59.71 ARV.dial :MM2 @23:01:01.21 delay 2 seconds @23:01:03.24 Adding MM2 @23:01:03.30 AS.seek 0.524ml @23:01:21.59 delay 25 seconds @23:01:46.61 ARV.dial :PP4 @23:01:47.01 Adding PP4 @23:01:47.03 delay 2 seconds @23:01:49.09 AS.seek 0.53ml @23:01:55.52 delay 10 seconds @23:02:05.54 ARV.dial :air3 @23:02:05.93 adding ARV air @23:02:05.95 delay 2 seconds @23:02:08.01 AS.seek 0.536ml @23:02:14.55 delay 5 seconds @23:02:19.57 ARV.dial :carrier @23:02:20.87 delay 7 seconds @23:02:27.95 AS.seek 0.594ml @23:03:26.52 delay 5 seconds @23:03:31.54 ATV.dial :elutionWater @23:03:33.01 delay 2 seconds @23:03:35.08 AS.seek 0.6ml @23:03:41.51 delay 4 seconds @23:03:45.53 ATV.dial ARV @23:03:46.96 delay 2 seconds @23:03:48.98 ARV.dial :carrier @23:03:48.99 delay 2 seconds @23:03:51.06 AS.seek 0.65ml @23:04:41.60 delay 4 seconds @23:04:45.62 ATV.dial PCR @23:04:46.38 ASV.dial :waste1 @23:04:46.39 ARV.dial :air1 @23:04:46.78 Positioning Slug by 0.324ml @23:04:46.86 AS.seek 0.326ml @23:10:12.79 delay 5 seconds @23:10:17.81 ATV.dial ARV @23:10:18.59 ASV.dial :PCRlock @23:10:18.96 delay 5 seconds @23:10:23.99 Running Tt1 Profile60C300 thermocycling @23:10:24.00 PCR.program Profile60C300 @23:10:24.13 Profile60C300 should complete in < 1:45:21 @23:10:24.14 PCR.run 2:16:41.25 @00:31:10.70CEST30-Aug-18 PCR.off @00:31:12.21 PCR for Tt1 completed -- closed /var/log/esp/Tt118aug2922h.pcr @00:31:12.27 Cleaning PCR @00:31:12.28 AFV.select :carrierSolution @00:31:12.39 delay 2 seconds @00:31:14.41 AS.reconfigure ASconfig @00:31:14.46 AS.seek 0.5ml @00:31:24.74 delay 1 second @00:31:25.76 AFV.select :holdingCoil @00:31:25.86 ASV.dial :waste1 @00:31:26.24 ATV.dial :air3 @00:31:27.54 AS.reconfigure ASslow1 @00:31:27.58 AS.seek 0.51ml @00:31:29.85 delay 2 seconds @00:31:31.87 ATV.dial :bleach @00:31:32.48 AS.seek 0.81ml @00:32:33.17 delay 3 seconds @00:32:36.19 ATV.dial :air3 @00:32:36.73 delay 3 seconds @00:32:39.80 AS.seek 0.835ml @00:32:45.12 delay 3 seconds @00:32:48.14 ATV.dial :bleach @00:32:48.74 AS.seek 0.82ml @00:32:52.04 delay 3 seconds @00:32:55.06 ARV.dial :waste @00:32:55.68 delay 3 seconds @00:32:58.70 ATV.dial :ARV @00:32:59.59 AS.reconfigure ASconfig @00:32:59.63 AS.seek 0.47ml @00:33:20.03 AFV.select :carrierSolution @00:33:20.14 delay 2 seconds @00:33:22.16 AS.seek 1ml @00:33:52.90 delay 1 second @00:33:53.92 AFV.select :holdingCoil @00:33:54.03 delay 1 second @00:33:55.05 AS.reconfigure ASfast @00:33:55.10 AS.seek 0ml @00:34:22.43 AFV.select :carrierSolution @00:34:22.54 delay 2 seconds @00:34:24.56 AS.reconfigure ASconfig @00:34:24.61 AS.seek 1ml @00:35:22.43 delay 1 second @00:35:23.45 AFV.select :holdingCoil @00:35:23.56 delay 1 second @00:35:24.58 AS.reconfigure ASfast @00:35:24.63 AS.seek 0ml @00:35:52.20 AFV.select :carrierSolution @00:35:52.31 delay 2 seconds @00:35:54.33 AS.reconfigure ASconfig @00:35:54.38 AS.seek 1ml @00:36:52.10 delay 1 second @00:36:53.12 AFV.select :holdingCoil @00:36:53.23 delay 1 second @00:36:54.25 AS.reconfigure ASfast @00:36:54.30 AS.seek 0ml @00:37:22.10 ARV.dial between carrier and air1 @00:37:22.60 ATV.dial :air3 @00:37:23.90 AS.reconfigure ASslow1 @00:37:23.94 AS.seek 0.01ml @00:37:26.21 delay 2 seconds @00:37:28.23 ATV.dial :bleach @00:37:28.91 AS.seek 0.81ml @00:40:10.04 delay 3 seconds @00:40:13.06 ATV.dial :air3 @00:40:13.61 delay 3 seconds @00:40:16.68 AS.seek 0.835ml @00:40:21.97 delay 3 seconds @00:40:24.99 ATV.dial :bleach @00:40:25.60 AS.seek 0.82ml @00:40:28.84 delay 3 seconds @00:40:31.86 ATV.dial PCR @00:40:32.26 AS.reconfigure ASconfig @00:40:32.31 AS.seek :empty @00:41:19.79 PCR.program :bleachProfile @00:41:19.84 PCR program should complete in < 2:45 @00:41:19.85 PCR.run 10:00 @00:44:21.67 PCR.off @00:44:21.70 AFV.select :carrierSolution @00:44:21.80 delay 2 seconds @00:44:23.82 AS.seek 1ml @00:45:21.62 delay 1 second @00:45:22.64 AFV.select :holdingCoil @00:45:22.75 delay 1 second @00:45:23.77 AS.reconfigure ASfast @00:45:23.82 AS.seek 0ml @00:45:51.53 delay 3 seconds @00:45:54.55 AFV.select :carrierSolution @00:45:54.66 delay 2 seconds @00:45:56.68 AS.reconfigure ASconfig @00:45:56.74 AS.seek 1ml @00:46:54.50 delay 1 second @00:46:55.52 AFV.select :holdingCoil @00:46:55.63 delay 1 second @00:46:56.65 AS.reconfigure ASfast @00:46:56.70 AS.seek 0ml @00:47:24.25 delay 3 seconds @00:47:27.27 AFV.select :carrierSolution @00:47:27.38 delay 2 seconds @00:47:29.40 AS.reconfigure ASconfig @00:47:29.45 AS.seek 1ml @00:48:27.28 delay 1 second @00:48:28.30 AFV.select :holdingCoil @00:48:28.41 delay 1 second @00:48:29.43 AS.reconfigure ASfast @00:48:29.48 AS.seek 0ml @00:48:57.22 delay 3 seconds @00:49:00.24 AFV.select :carrierSolution @00:49:00.35 delay 2 seconds @00:49:02.37 AS.reconfigure ASconfig @00:49:02.43 AS.seek 1ml @00:50:00.25 delay 1 second @00:50:01.27 AFV.select :holdingCoil @00:50:01.38 delay 1 second @00:50:02.40 AS.reconfigure ASfast @00:50:02.45 AS.seek 0ml @00:50:30.04 delay 3 seconds @00:50:33.06 AFV.select :carrierSolution @00:50:33.17 delay 2 seconds @00:50:35.19 AS.reconfigure ASconfig @00:50:35.25 AS.seek 1ml @00:51:33.08 delay 1 second @00:51:34.10 AFV.select :holdingCoil @00:51:34.21 delay 1 second @00:51:35.23 AS.reconfigure ASnorm @00:51:35.28 AS.seek 0ml @15:39:56.95CEST06-Sep-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @15:39:59.47 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @15:39:59.76 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @15:40:05.42 Polling Can every 10:00 @15:40:07.27 SPE.coast @15:40:07.33 SPE.configure SPEconfig @15:40:07.42 AS.reconfigure ASconfig @15:40:07.47 AS.hold @15:40:07.54 AS.configure ASconfig @15:40:07.64 SS.reconfigure SSconfig @15:40:07.69 SS.hold @15:40:07.75 SS.configure SSconfig @15:40:07.85 CC.reconfigure CCconfig @15:40:07.90 CC.hold @15:40:07.95 CC.configure CCconfig @15:40:08.05 CS.reconfigure CSconfig @15:40:08.09 CS.hold @15:40:08.18 CS.configure CSconfig @15:40:08.67 PC.reconfigure PCconfig @15:40:08.72 PC.hold @15:40:08.79 PC.configure PCconfig @15:40:08.89 PS.configure PSconfig @15:40:09.00 Forearm.reconfigure LooseForearm @15:40:09.05 Forearm.hold @15:40:09.12 Forearm.configure LooseForearm @15:40:09.22 Elbow.reconfigure LooseElbow @15:40:09.26 Elbow.hold @15:40:09.32 Elbow.configure LooseElbow @15:40:09.43 SC.configure SCloose @15:40:09.53 SE.configure SEconfig @15:40:09.57 CH.coast @15:40:09.63 CH.configure CHconfig @15:40:09.66 PH.coast @15:40:09.72 PH.configure PHconfig @15:40:09.76 PCR.off @15:40:09.79 PCR.pidtemp= 50,0.1,0,300,25,25 @15:40:09.82 PCR.caltemp= 2.7073,0.0373,0 @15:40:09.85 All dwarf controllers running firmware version 3.75 @15:40:10.02 Cleanly exited at 15:01:46CEST06-Sep-18 @15:40:10.06 Waiting for espclients... @15:40:45.90 Session Begun @15:40:56.40 -> pcrDecon @15:40:56.50 Cleaning PCR @15:40:56.51 AFV.select :carrierSolution @15:40:56.67 delay 2 seconds @15:40:58.71 AS.seek 0.5ml @15:41:09.04 delay 1 second @15:41:10.06 AFV.select :holdingCoil @15:41:10.18 ASV.dial :waste1 @15:41:10.60 ATV.dial :air3 @15:41:11.91 AS.reconfigure ASslow1 @15:41:11.99 AS.seek 0.51ml @15:41:14.31 delay 2 seconds @15:41:16.34 ATV.dial :bleach @15:41:16.99 AS.seek 0.81ml @15:42:17.63 delay 3 seconds @15:42:20.66 ATV.dial :air3 @15:42:21.21 delay 3 seconds @15:42:24.30 AS.seek 0.835ml @15:42:29.63 delay 3 seconds @15:42:32.66 ATV.dial :bleach @15:42:33.31 AS.seek 0.82ml @15:42:36.63 delay 3 seconds @15:42:39.66 ARV.dial :waste @15:42:40.29 delay 3 seconds @15:42:43.32 ATV.dial :ARV @15:42:44.34 AS.reconfigure ASconfig @15:42:44.42 AS.seek 0.47ml @15:43:04.87 AFV.select :carrierSolution @15:43:05.00 delay 2 seconds @15:43:07.04 AS.seek 1ml @15:43:37.83 delay 1 second @15:43:38.85 AFV.select :holdingCoil @15:43:38.98 delay 1 second @15:43:40.02 AS.reconfigure ASfast @15:43:40.09 AS.seek 0ml @15:44:07.46 AFV.select :carrierSolution @15:44:07.59 delay 2 seconds @15:44:09.63 AS.reconfigure ASconfig @15:44:09.70 AS.seek 1ml @15:45:07.51 delay 1 second @15:45:08.53 AFV.select :holdingCoil @15:45:08.66 delay 1 second @15:45:09.69 AS.reconfigure ASfast @15:45:09.76 AS.seek 0ml @15:45:37.12 AFV.select :carrierSolution @15:45:37.25 delay 2 seconds @15:45:39.29 AS.reconfigure ASconfig @15:45:39.36 AS.seek 1ml @15:46:37.15 delay 1 second @15:46:38.17 AFV.select :holdingCoil @15:46:38.29 delay 1 second @15:46:39.33 AS.reconfigure ASfast @15:46:39.40 AS.seek 0ml @15:47:06.77 ARV.dial between carrier and air1 @15:47:07.29 ATV.dial :air3 @15:47:08.60 AS.reconfigure ASslow1 @15:47:08.68 AS.seek 0.01ml @15:47:11.08 delay 2 seconds @15:47:13.10 ATV.dial :bleach @15:47:13.74 AS.seek 0.81ml @15:49:54.93 delay 3 seconds @15:49:57.96 ATV.dial :air3 @15:49:58.54 delay 3 seconds @15:50:01.63 AS.seek 0.835ml @15:50:06.93 delay 3 seconds @15:50:09.95 ATV.dial :bleach @15:50:10.59 AS.seek 0.82ml @15:50:13.90 delay 3 seconds @15:50:16.93 ATV.dial PCR @15:50:17.36 AS.reconfigure ASconfig @15:50:17.42 AS.seek :empty @15:51:04.88 PCR.program :bleachProfile @15:51:04.96 PCR program should complete in < 2:45 @15:51:04.97 PCR.run 10:00 @15:54:00.76 PCR.off @15:54:00.80 AFV.select :carrierSolution @15:54:00.94 delay 2 seconds @15:54:02.98 AS.seek 1ml @15:55:00.83 delay 1 second @15:55:01.85 AFV.select :holdingCoil @15:55:01.98 delay 1 second @15:55:03.02 AS.reconfigure ASfast @15:55:03.10 AS.seek 0ml @15:55:30.48 delay 3 seconds @15:55:33.52 AFV.select :carrierSolution @15:55:33.65 delay 2 seconds @15:55:35.69 AS.reconfigure ASconfig @15:55:35.76 AS.seek 1ml @15:56:33.57 delay 1 second @15:56:34.60 AFV.select :holdingCoil @15:56:34.77 delay 1 second @15:56:35.84 AS.reconfigure ASfast @15:56:35.99 AS.seek 0ml @15:57:03.43 delay 3 seconds @15:57:06.48 AFV.select :carrierSolution @15:57:06.66 delay 2 seconds @15:57:08.74 AS.reconfigure ASconfig @15:57:08.88 AS.seek 1ml @15:58:06.74 delay 1 second @15:58:07.77 AFV.select :holdingCoil @15:58:07.92 delay 1 second @15:58:08.99 AS.reconfigure ASfast @15:58:09.11 AS.seek 0ml @15:58:36.55 delay 3 seconds @15:58:39.57 AFV.select :carrierSolution @15:58:39.69 delay 2 seconds @15:58:41.73 AS.reconfigure ASconfig @15:58:41.80 AS.seek 1ml @15:59:39.57 delay 1 second @15:59:40.59 AFV.select :holdingCoil @15:59:40.71 delay 1 second @15:59:41.75 AS.reconfigure ASfast @15:59:41.82 AS.seek 0ml @16:00:09.18 delay 3 seconds @16:00:12.21 AFV.select :carrierSolution @16:00:12.33 delay 2 seconds @16:00:14.37 AS.reconfigure ASconfig @16:00:14.43 AS.seek 1ml @16:01:12.19 delay 1 second @16:01:13.21 AFV.select :holdingCoil @16:01:13.33 delay 1 second @16:01:14.37 AS.reconfigure ASnorm @16:01:14.44 AS.seek 0ml @16:04:58.54 -> shortmfb([Tt1],[:elutionWater]){}.finish @16:04:58.75 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep0616h.pcr @16:04:58.79 Building PCR Slug from elutionWater with MM2 mix, PP4 primer @16:04:58.83 Priming with carrier @16:04:58.85 ATV.dial ARV @16:04:59.49 ARV.dial :carrier @16:04:59.87 AS.seek 0.5ml @16:05:25.44 AFV.select :carrierSolution @16:05:25.58 delay 2 seconds @16:05:27.61 AS.reconfigure ASconfig @16:05:27.68 AS.seek 1ml @16:05:56.72 delay 1 second @16:05:58.12 AFV.select :holdingCoil @16:05:58.34 delay 1 second @16:05:59.36 ATV.dial :waste @16:06:01.03 AS.reconfigure ASnorm @16:06:01.10 AS.seek :empty @16:06:51.90 AFV.select :carrierSolution @16:06:52.03 delay 2 seconds @16:06:54.07 AS.reconfigure ASconfig @16:06:54.13 AS.seek 0.5ml @16:07:23.25 delay 1 second @16:07:24.28 AFV.select :holdingCoil @16:07:24.41 Building Slug @16:07:24.42 ATV.dial :ARV @16:07:26.08 delay 2 seconds @16:07:28.10 ARV.dial :air1 @16:07:28.53 delay 2 seconds @16:07:30.61 AS.reconfigure ASslow2 @16:07:30.69 AS.seek 0.506ml @16:07:37.07 delay 2 seconds @16:07:39.12 ARV.dial :MM2 @16:07:40.68 delay 2 seconds @16:07:42.72 Adding MM2 @16:07:42.81 AS.seek 0.524ml @16:07:53.98 Session Begun @16:07:55.86 -> can @16:08:01.47 delay 25 seconds @16:08:14.00 -> exit @16:08:14.02 Session Ended @16:08:26.50 ARV.dial :PP4 @16:08:26.94 Adding PP4 @16:08:26.95 delay 2 seconds @16:08:29.04 AS.seek 0.53ml @16:08:35.47 delay 10 seconds @16:08:45.50 ARV.dial :air3 @16:08:45.95 adding ARV air @16:08:45.96 delay 2 seconds @16:08:48.05 AS.seek 0.536ml @16:08:54.40 delay 5 seconds @16:08:59.43 ARV.dial :carrier @16:09:00.74 delay 7 seconds @16:09:07.83 AS.seek 0.594ml @16:10:06.44 delay 5 seconds @16:10:11.47 ATV.dial :elutionWater @16:10:12.91 delay 2 seconds @16:10:14.99 AS.seek 0.6ml @16:10:21.46 delay 4 seconds @16:10:25.49 ATV.dial ARV @16:10:27.00 delay 2 seconds @16:10:29.03 ARV.dial :carrier @16:10:29.05 delay 2 seconds @16:10:31.14 AS.seek 0.65ml @16:11:21.94 delay 4 seconds @16:11:25.97 ATV.dial PCR @16:11:26.74 ASV.dial :waste1 @16:11:26.76 ARV.dial :air1 @16:11:27.21 Positioning Slug by 0.324ml @16:11:27.32 AS.seek 0.326ml @16:16:53.24 delay 5 seconds @16:16:58.27 ATV.dial ARV @16:16:59.10 ASV.dial :PCRlock @16:16:59.52 delay 5 seconds @16:17:04.56 Running Tt1 Profile60C300 thermocycling @16:17:04.57 PCR.program Profile60C300 @16:17:04.74 Profile60C300 should complete in < 1:45:21 @16:17:04.76 PCR.run 2:16:41.25 @17:41:59.34 PCR.off @17:41:59.38 Errno::EPIPE in bkha's_reader -- Broken pipe @17:41:59.42 Session Ended @17:42:00.95 PCR for Tt1 completed -- closed /var/log/esp/Tt118sep0616h.pcr @17:42:01.03 Cleaning PCR @17:42:01.04 AFV.select :carrierSolution @17:42:01.14 delay 2 seconds @17:42:03.18 AS.reconfigure ASconfig @17:42:03.22 AS.seek 0.5ml @17:42:13.55 delay 1 second @17:42:14.57 AFV.select :holdingCoil @17:42:14.67 ASV.dial :waste1 @17:42:15.14 ATV.dial :air3 @17:42:16.43 AS.reconfigure ASslow1 @17:42:16.48 AS.seek 0.51ml @17:42:18.76 delay 2 seconds @17:42:20.78 ATV.dial :bleach @17:42:21.40 AS.seek 0.81ml @17:43:22.00 delay 3 seconds @17:43:25.03 ATV.dial :air3 @17:43:25.56 delay 3 seconds @17:43:28.65 AS.seek 0.835ml @17:43:34.01 delay 3 seconds @17:43:37.03 ATV.dial :bleach @17:43:37.65 AS.seek 0.82ml @17:43:40.95 delay 3 seconds @17:43:43.98 ARV.dial :waste @17:43:44.59 delay 3 seconds @17:43:47.62 ATV.dial :ARV @17:43:48.51 AS.reconfigure ASconfig @17:43:48.56 AS.seek 0.47ml @17:44:08.97 AFV.select :carrierSolution @17:44:09.08 delay 2 seconds @17:44:11.12 AS.seek 1ml @17:44:42.34 delay 1 second @17:44:43.36 AFV.select :holdingCoil @17:44:43.46 delay 1 second @17:44:44.50 AS.reconfigure ASfast @17:44:44.54 AS.seek 0ml @17:45:11.87 AFV.select :carrierSolution @17:45:11.98 delay 2 seconds @17:45:14.02 AS.reconfigure ASconfig @17:45:14.06 AS.seek 1ml @17:46:11.86 delay 1 second @17:46:12.88 AFV.select :holdingCoil @17:46:12.98 delay 1 second @17:46:14.02 AS.reconfigure ASfast @17:46:14.07 AS.seek 0ml @17:46:41.42 AFV.select :carrierSolution @17:46:41.53 delay 2 seconds @17:46:43.57 AS.reconfigure ASconfig @17:46:43.61 AS.seek 1ml @17:47:41.40 delay 1 second @17:47:42.42 AFV.select :holdingCoil @17:47:42.52 delay 1 second @17:47:43.56 AS.reconfigure ASfast @17:47:43.60 AS.seek 0ml @17:48:10.95 ARV.dial between carrier and air1 @17:48:11.45 ATV.dial :air3 @17:48:12.74 AS.reconfigure ASslow1 @17:48:12.79 AS.seek 0.01ml @17:48:15.15 delay 2 seconds @17:48:17.18 ATV.dial :bleach @17:48:17.80 AS.seek 0.81ml @17:50:59.11 delay 3 seconds @17:51:02.13 ATV.dial :air3 @17:51:02.67 delay 3 seconds @17:51:05.76 AS.seek 0.835ml @17:51:11.12 delay 3 seconds @17:51:14.15 ATV.dial :bleach @17:51:14.78 AS.seek 0.82ml @17:51:18.09 delay 3 seconds @17:51:21.12 ATV.dial PCR @17:51:21.52 AS.reconfigure ASconfig @17:51:21.56 AS.seek :empty @17:52:09.05 PCR.program :bleachProfile @17:52:09.11 PCR program should complete in < 2:45 @17:52:09.12 PCR.run 10:00 @17:55:01.85 PCR.off @17:55:01.88 AFV.select :carrierSolution @17:55:01.99 delay 2 seconds @17:55:04.03 AS.seek 1ml @17:56:01.83 delay 1 second @17:56:02.85 AFV.select :holdingCoil @17:56:02.95 delay 1 second @17:56:03.99 AS.reconfigure ASfast @17:56:04.03 AS.seek 0ml @17:56:31.39 delay 3 seconds @17:56:34.42 AFV.select :carrierSolution @17:56:34.52 delay 2 seconds @17:56:36.56 AS.reconfigure ASconfig @17:56:36.61 AS.seek 1ml @17:57:34.39 delay 1 second @17:57:35.41 AFV.select :holdingCoil @17:57:35.51 delay 1 second @17:57:36.55 AS.reconfigure ASfast @17:57:36.59 AS.seek 0ml @17:58:03.95 delay 3 seconds @17:58:06.98 AFV.select :carrierSolution @17:58:07.08 delay 2 seconds @17:58:09.12 AS.reconfigure ASconfig @17:58:09.18 AS.seek 1ml @17:59:06.97 delay 1 second @17:59:07.99 AFV.select :holdingCoil @17:59:08.09 delay 1 second @17:59:09.13 AS.reconfigure ASfast @17:59:09.17 AS.seek 0ml @17:59:36.53 delay 3 seconds @17:59:39.56 AFV.select :carrierSolution @17:59:39.66 delay 2 seconds @17:59:41.70 AS.reconfigure ASconfig @17:59:41.74 AS.seek 1ml @18:00:39.53 delay 1 second @18:00:40.55 AFV.select :holdingCoil @18:00:40.65 delay 1 second @18:00:41.69 AS.reconfigure ASfast @18:00:41.73 AS.seek 0ml @18:01:09.08 delay 3 seconds @18:01:12.11 AFV.select :carrierSolution @18:01:12.21 delay 2 seconds @18:01:14.25 AS.reconfigure ASconfig @18:01:14.30 AS.seek 1ml @18:02:12.04 delay 1 second @18:02:13.06 AFV.select :holdingCoil @18:02:13.16 delay 1 second @18:02:14.20 AS.reconfigure ASnorm @18:02:14.24 AS.seek 0ml @19:08:58.75 Session Begun @19:09:20.08 -> exit @19:09:20.10 Session Ended @19:10:40.45 Session Begun @19:10:59.50 -> execute "18tun.rb" @19:10:59.61 GoodNews.email "Starting 18tun w/pucks in tube 4\n{1 => 0, 2 => 0, 3 => 0, 4 => 2, 5 => 0, 6 => 0, 7 => 0}\nCan@19:10:06, 24.3C, 63% humidity, 14.7psia, 11.996V, 0.695A, 0.695A avg, 8.34W",Subject: "18tun begun" @19:10:59.66 Preparing to sleep until +0:00:15 @19:10:59.75 AS.coast @19:10:59.78 Asleep til +0:00:15 @19:10:59.87 Continuing at 19:11 @19:10:59.89 SS.coast @19:11:00.05 CC.coast @19:11:00.10 CS.coast @19:11:00.17 PC.coast @19:11:00.23 Forearm.coast @19:11:00.29 Elbow.coast @19:11:00.72 Gate.power :core,:OFF @19:11:00.74 Gate.power :raw,:OFF @19:11:00.76 Shutdown @19:11:05.77 Powering core bus @19:11:08.71 GoodNews.email "Then, in tube 4: coreNEG\n/home/esp/esp2/mission/18tun.rb:106\n{1 => 0, 2 => 0, 3 => 0, 4 => 2, 5 => 0, 6 => 0, 7 => 0}\nCan@19:10:59, 24.3C, 63% humidity, 14.7psia, 12.001V, 0.676A, 0.676A avg, 8.11W",Subject: "Asleep until 19:11" @19:11:10.95 Gate.power :raw,:ON @19:11:11.73 SPE.coast @19:11:11.84 SPE.configure SPEconfig @19:11:11.97 AS.configure ASconfig @19:11:12.12 SS.configure SSconfig @19:11:12.26 CC.configure CCconfig @19:11:12.41 CS.configure CSconfig @19:11:12.58 PC.configure PCconfig @19:11:12.73 PS.configure PSconfig @19:11:12.92 Forearm.configure LooseForearm @19:11:13.07 Elbow.configure LooseElbow @19:11:13.22 SC.configure SCloose @19:11:13.38 SE.configure SEconfig @19:11:13.47 CH.coast @19:11:13.54 CH.configure CHconfig @19:11:13.63 PH.coast @19:11:13.73 PH.configure PHconfig @19:11:13.80 PCR.off @19:11:13.91 PCR.pidtemp= 50,0.1,0,300,25,25 @19:11:14.00 PCR.caltemp= 2.7073,0.0373,0 @19:11:14.04 All dwarf controllers running firmware version 3.75 @19:11:14.24 Valve.reset @19:11:14.46 PCR.off @19:11:14.53 PCR.pidtemp= 50,0.1,0,300,25,25 @19:11:14.57 PCR.caltemp= 2.7073,0.0373,0 @19:11:14.70 Intake.select :close @19:11:14.82 Exhaust.select :close @19:11:14.86 CTV.dial :air @19:11:15.01 PSV.dial :air @19:11:15.06 CSV.dial :waste1 @19:11:15.30 SSV.dial :spit,avoiding: :exhaust @19:11:15.38 PS.reconfigure PSconfig @19:11:15.41 Forearm.reconfigure LooseForearm @19:11:15.66 PS.home.seek 0ml @19:11:15.70 Forearm.home.seek :retract @19:11:15.96 CS.reconfigure CSfast @19:11:16.02 SS.reconfigure SSconfig @19:11:16.14 CS.home.seek 0ml @19:11:16.18 SS.home.seek 0ml @19:11:16.57 Hand.close @19:11:16.61 Elbow.jog 1500 @19:11:17.22 Elbow.reconfigure LooseElbow @19:11:17.30 Elbow.home.seek :clear @19:11:21.99 SE.reconfigure SEconfig @19:11:22.06 SE.home.seek :down @19:11:22.80 SC.reconfigure SCsloppy @19:11:22.87 SC.home.seek :initial @19:11:25.01 PS.coast @19:11:34.75 Forearm.seek :retract @19:11:34.87 Elbow.seek :hammer @19:11:36.05 Forearm.reconfigure TightForearm @19:11:36.13 Forearm.seek 5 + 350 counts @19:11:37.21 Forearm.seek :retract @19:11:38.47 CC.reconfigure CCconfig @19:11:38.49 PC.reconfigure PCconfig @19:11:38.62 CC.home.seek :open @19:11:38.66 PC.home.seek :open @19:11:42.15 Phase [coreNEG] @19:11:42.17 GoodNews.email "At 19:11:42 began in tube 4: coreNEG\n/home/esp/esp2/mission/18tun.rb:106\n{1 => 0, 2 => 0, 3 => 0, 4 => 2, 5 => 0, 6 => 0, 7 => 0}\nCan@19:11:42, 24.4C, 63% humidity, 14.7psia, 11.972V, 0.802A, 0.802A avg, 9.60W",Subject: "Started 18tun phase coreNEG" @19:11:42.23CEST06-Sep-18 ==================> STARTED coreNEG (phase #1) <=== @19:11:42.26 Starting initialPurge @19:11:42.29 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:11:42.40 Hand.open @19:11:43.32 Elbow.reconfigure TightElbow @19:11:43.41 Elbow.seek FlushPuck::Garage @19:11:45.34 Elbow.reconfigure LooseElbow @19:11:45.51 Forearm.reconfigure LooseForearm @19:11:45.59 Forearm.seek :garage @19:11:46.67 Hand.close @19:11:47.59 Forearm.seek :retract @19:11:48.71 Elbow.reconfigure TightElbow @19:11:48.78 Elbow.seek CC @19:11:51.71 Elbow.reconfigure LooseElbow @19:11:51.85 Forearm.reconfigure TightForearm @19:11:51.92 Forearm.seek CC @19:11:53.26 CC.seek :closed @19:11:57.60 Collection Clamp onSpring/offSpring for 5/30 samples, on/off torque 2.34 @19:11:57.73 Hand.open @19:11:58.62 Forearm.reconfigure LooseForearm @19:11:58.70 Forearm.seek :retract @19:11:59.74 Flushing the Collection Valve::Manifold @19:11:59.92 CSV.dial CSR @19:12:01.48 Pulling 5ml of CSR.flush @19:12:01.49 CSR.select :flush @19:12:01.66 CS.reconfigure CSconfig @19:12:01.75 CS.seek 5ml @19:12:15.56 delay 5 seconds @19:12:20.58 CSR.select :air @19:12:20.77 CS.reconfigure CSfast @19:12:20.85 CS.seek 7.5ml @19:12:28.27 CSV.dial :waste @19:12:29.77 CS.reconfigure CSconfig @19:12:29.85 CS.seek 0ml @19:12:50.05 Purging external Intake @19:12:50.22 CSV.dial :flush @19:12:50.74 CS.reconfigure CSfast @19:12:50.82 CS.seek 10ml @19:13:17.40 delay 5 seconds @19:13:17.43 Errno::EPIPE in mwj's_reader -- Broken pipe @19:13:17.45 Session Ended @19:13:34.47 Session Begun @19:14:44.14 Session Ended @19:26:22.53 Session Begun @19:26:33.10 -> showlog 20 @19:28:18.17 -> showlog 50 @19:38:52.61 Session Begun @19:38:56.48 -> showlog 100 @19:58:41.39 Session Begun @19:58:50.90 -> showlog 100 @20:02:41.59 -> Flush @20:07:31.35 -> CBV @20:08:43.08 -> Intake @20:08:49.67 -> CBV.waste1 @20:08:49.70 CBV.dial :waste1 @20:09:01.92 -> Flush @20:10:25.43 Session Begun @20:10:35.01 -> #Hi! @20:11:18.94 -> #I've moved the CBV to :waste1 to relieve pressure. @20:11:37.71 -> #Did the nub pop off the intake? @20:12:01.88 -> #hi @20:12:12.35 -> #If not, you prabably need to uncan to determine whether anything broke inside the ESP @20:12:20.18 -> #no, the nubs are still on and i do not see any fluid @20:12:46.92 -> # i did not expect to either as it stopped right before it would push flush out the intake @20:13:10.03 -> #Yes, you were quite lucky! @20:13:11.20 -> showlog 100 @20:13:55.55 -> #OK. No big deal then. Take the nubs off and verify that we can push the slug of flush out the intake @20:14:43.65 Session Ended @20:14:47.25 -> #yes! for future references would the pressure sensors not overcurrent if it had pushed? @20:15:42.79 -> #Probably, but the CS is small enough to generate a lot of pressure before then. It might pop a fitting. @20:16:37.26 -> #When you take the nubs off, I'll try to push the flush out. Watch the intake as I do. @20:17:06.33 -> #Jesus, this was a close one then! @20:17:25.22 -> #okay give me 5 mins Ill plug up the ESP @20:17:32.33 -> #wait for my go @20:18:03.54 -> #fine. But, we're trying to unplug the ESP, not plug it up more. Right? @20:18:54.92 -> CS @20:24:51.64 -> #okay, intake and exhaust have been opened with coilsleading to a waste bucket @20:25:09.26 -> #correct, we are opening the intake and exhaust. @20:25:33.59 -> CBV.connect @20:25:33.62 ArgumentError in brent -- wrong number of arguments (0 for 1) @20:25:38.04 -> CBV.connect CSV @20:25:38.06 CBV.dial CSV @20:25:38.12 CSV.dial CBV @20:25:44.99 -> #no pressure was on the intake and exhaust @20:25:49.59 -> CTV.intake @20:25:49.61 CTV.dial :intake @20:25:57.73 -> Intake.open @20:25:57.75 Intake.select :open @20:26:33.82 -> #Watch the Intake now! @20:26:39.59 -> CS.empty @20:26:39.63 CS.reconfigure CSconfig @20:26:39.76 CS.seek :empty @20:27:11.42 -> Intake.close @20:27:11.44 Intake.select :close @20:27:37.06 -> #Any fluid come out? @20:28:32.16 -> #yes @20:29:05.77 -> # did not see from which, but I can see that some is in the bucket @20:32:14.28 -> #Good. I'll just garage the puck and you can proceed. @20:32:21.62 -> Flush.garage @20:32:21.65 ThreadError in brent -- mwj thread died holding FlushPuck @20:32:21.72 Garaging FlushPuck in Collection Clamp @20:32:21.87 Elbow.reconfigure TightElbow @20:32:21.99 Elbow.seek CC @20:32:22.35 Elbow.reconfigure LooseElbow @20:32:22.52 Forearm.seek CC @20:32:23.56 Hand.close @20:32:24.39 CC.seek :open @20:32:28.88 Forearm.seek :retract @20:32:29.93 Elbow.reconfigure TightElbow @20:32:30.02 Elbow.seek :garage @20:32:33.26 Elbow.reconfigure LooseElbow @20:32:33.42 Forearm.reconfigure TightForearm @20:32:33.51 Forearm.seek :garage @20:32:34.75 Hand.open @20:32:35.58 Forearm.reconfigure LooseForearm @20:32:35.68 Forearm.seek :retract @20:32:55.33 -> Thread[:mwj] @20:33:04.46 -> #Who is mwj? @20:33:15.00 -> # its Magnus @20:33:28.91 -> # my collegue, yousaw him yesterday @20:34:10.05 -> #Yes, I thought it might be he. Ok, you're good to proceed. @20:34:16.63 -> #Bye! @20:34:19.07 -> # Ugh, this was a close one, than Brent. I'll stop this session and start a new one @20:34:27.24 -> exit @20:34:27.26 Session Ended @20:35:12.57 -> abort! "ESP reset, starting new session" @20:35:12.59 CmdServer::Client::Error in bkha2 -- Aborting Mission: ESP reset, starting new session @20:35:12.69 BadNews.email "ESP reset, starting new session",Subject: "18tun ABORTED!" @20:35:20.26 Session Ended @20:35:20.31 Session Ended @20:37:27.92CEST06-Sep-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @20:37:30.44 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @20:37:30.74 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @20:37:36.91 Polling Can every 10:00 @20:37:38.88 SPE.coast @20:37:39.00 SPE.configure SPEconfig @20:37:39.22 AS.configure ASconfig @20:37:39.42 SS.reconfigure SSconfig @20:37:39.52 SS.hold @20:37:39.64 SS.configure SSconfig @20:37:39.77 CC.reconfigure CCconfig @20:37:39.82 CC.hold @20:37:39.88 CC.configure CCconfig @20:37:39.97 CS.reconfigure CSconfig @20:37:40.02 CS.hold @20:37:40.10 CS.configure CSconfig @20:37:40.20 PC.reconfigure PCconfig @20:37:40.25 PC.hold @20:37:40.72 PC.configure PCconfig @20:37:40.82 PS.configure PSconfig @20:37:40.96 Forearm.reconfigure LooseForearm @20:37:41.00 Forearm.hold @20:37:41.06 Forearm.configure LooseForearm @20:37:41.16 Elbow.reconfigure LooseElbow @20:37:41.21 Elbow.hold @20:37:41.27 Elbow.configure LooseElbow @20:37:41.39 SC.reconfigure SCloose @20:37:41.44 SC.hold @20:37:41.50 SC.configure SCloose @20:37:41.60 SE.reconfigure SEconfig @20:37:41.64 SE.hold @20:37:41.73 SE.configure SEconfig @20:37:41.83 CH.coast @20:37:41.95 CH.configure CHconfig @20:37:42.01 PH.coast @20:37:42.14 PH.configure PHconfig @20:37:42.23 PCR.off @20:37:42.29 PCR.pidtemp= 50,0.1,0,300,25,25 @20:37:42.33 PCR.caltemp= 2.7073,0.0373,0 @20:37:42.37 All dwarf controllers running firmware version 3.75 @20:37:42.65 Cleanly exited at 20:35:20CEST06-Sep-18 @20:37:42.69 Loading /home/esp/esp2/mission/18tun.rb mission @20:37:42.76 Preparing to sleep until +0:00:15 @20:37:42.78 GoodNews.email "Starting 18tun w/pucks in tube 4\n{1 => 0, 2 => 0, 3 => 0, 4 => 2, 5 => 0, 6 => 0, 7 => 0}\nCan@20:37:36, 24.7C, 62% humidity, 14.7psia, 12.001V, 0.701A, 0.695A avg, 8.42W",Subject: "18tun begun" @20:37:42.87 SS.coast @20:37:42.90
Asleep til +0:00:15 @20:37:42.93 Continuing at 20:37 @20:37:42.97 CC.coast @20:37:43.07 CS.coast @20:37:43.14 PC.coast @20:37:43.18 Forearm.coast @20:37:43.21 Elbow.coast @20:37:43.25 SC.coast @20:37:43.30 SE.coast @20:37:43.70 Gate.power :core,:OFF @20:37:43.73 Gate.power :raw,:OFF @20:37:43.74 Shutdown @20:37:45.00 Session Begun @20:37:49.73 GoodNews.email "Then, in tube 4: coreNEG\n/home/esp/esp2/mission/18tun.rb:106\n{1 => 0, 2 => 0, 3 => 0, 4 => 2, 5 => 0, 6 => 0, 7 => 0}\nCan@20:37:43, 24.7C, 62% humidity, 14.7psia, 11.996V, 0.695A, 0.695A avg, 8.34W",Subject: "Asleep until 20:37" @20:37:52.32 -> showlog 100 @20:37:57.73
Powering core bus @20:38:02.58 Gate.power :raw,:ON @20:38:03.30 SPE.coast @20:38:03.39 SPE.configure SPEconfig @20:38:03.50 AS.configure ASconfig @20:38:03.63 SS.configure SSconfig @20:38:03.76 CC.configure CCconfig @20:38:03.88 CS.configure CSconfig @20:38:04.01 PC.configure PCconfig @20:38:04.14 PS.configure PSconfig @20:38:04.29 Forearm.configure LooseForearm @20:38:04.41 Elbow.configure LooseElbow @20:38:04.59 SC.configure SCloose @20:38:04.71 SE.configure SEconfig @20:38:04.78 CH.coast @20:38:04.85 CH.configure CHconfig @20:38:04.90 PH.coast @20:38:04.99 PH.configure PHconfig @20:38:05.05 PCR.off @20:38:05.10 PCR.pidtemp= 50,0.1,0,300,25,25 @20:38:05.13 PCR.caltemp= 2.7073,0.0373,0 @20:38:05.19 All dwarf controllers running firmware version 3.75 @20:38:05.31 Valve.reset @20:38:05.51 PCR.off @20:38:05.55 PCR.pidtemp= 50,0.1,0,300,25,25 @20:38:05.60 PCR.caltemp= 2.7073,0.0373,0 @20:38:05.77 Intake.select :close @20:38:05.87 Exhaust.select :close @20:38:05.88 CTV.dial :air @20:38:06.03 PSV.dial :air @20:38:06.14
Forearm.reconfigure LooseForearm @20:38:06.20 Forearm.home.seek :retract @20:38:06.29 PS.reconfigure PSconfig @20:38:06.37 PS.home.seek 0ml @20:38:06.84 CSV.dial :waste1 @20:38:06.96 Hand.close @20:38:07.01
Elbow.jog 1500 @20:38:07.38 SSV.dial :spit,avoiding: :exhaust @20:38:07.57 CS.reconfigure CSfast @20:38:07.61 SS.reconfigure SSconfig @20:38:07.77 CS.home.seek 0ml @20:38:07.80 SS.home.seek 0ml @20:38:07.95
Elbow.reconfigure LooseElbow @20:38:08.03 Elbow.home.seek :clear @20:38:11.43 SE.reconfigure SEconfig @20:38:11.50 SE.home.seek :down @20:38:12.32 SC.reconfigure SCsloppy @20:38:12.38 SC.home.seek :initial @20:38:16.09 PS.coast @20:38:16.41
Forearm.seek :retract @20:38:16.52 Elbow.seek :hammer @20:38:17.71 Forearm.reconfigure TightForearm @20:38:17.80 Forearm.seek 5 + 350 counts @20:38:18.90 Forearm.seek :retract @20:38:20.29 PC.reconfigure PCconfig @20:38:20.31
CC.reconfigure CCconfig @20:38:20.40 PC.home.seek :open @20:38:20.48
CC.home.seek :open @20:38:23.81 Phase [coreNEG] @20:38:23.83 GoodNews.email "At 20:38:23 began in tube 4: coreNEG\n/home/esp/esp2/mission/18tun.rb:106\n{1 => 0, 2 => 0, 3 => 0, 4 => 2, 5 => 0, 6 => 0, 7 => 0}\nCan@20:38:23, 24.8C, 62% humidity, 14.7psia, 11.913V, 1.036A, 1.029A avg, 12.34W",Subject: "Started 18tun phase coreNEG" @20:38:23.87CEST06-Sep-18
==================> STARTED coreNEG (phase #1) <=== @20:38:23.92 Starting initialPurge @20:38:23.95 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:38:24.05 Hand.open @20:38:25.05 Elbow.reconfigure TightElbow @20:38:25.13 Elbow.seek FlushPuck::Garage @20:38:27.51 Elbow.reconfigure LooseElbow @20:38:27.68 Forearm.reconfigure LooseForearm @20:38:27.74 Forearm.seek :garage @20:38:28.83 Hand.close @20:38:29.78 Forearm.seek :retract @20:38:30.83 Elbow.reconfigure TightElbow @20:38:30.91 Elbow.seek CC @20:38:33.84 Elbow.reconfigure LooseElbow @20:38:33.98 Forearm.reconfigure TightForearm @20:38:34.05 Forearm.seek CC @20:38:35.38 CC.seek :closed @20:38:39.62 Collection Clamp onSpring/offSpring for 6/30 samples, on/off torque 2.38 @20:38:39.74 Hand.open @20:38:40.57 Forearm.reconfigure LooseForearm @20:38:40.64 Forearm.seek :retract @20:38:41.67 Flushing the Collection Valve::Manifold @20:38:41.83 CSV.dial CSR @20:38:43.36 Pulling 5ml of CSR.flush @20:38:43.37 CSR.select :flush @20:38:43.53 CS.reconfigure CSconfig @20:38:43.60 CS.seek 5ml @20:38:57.32 delay 5 seconds @20:39:02.35 CSR.select :air @20:39:02.55 CS.reconfigure CSfast @20:39:02.62 CS.seek 7.5ml @20:39:10.01 CSV.dial :waste @20:39:11.50 CS.reconfigure CSconfig @20:39:11.57 CS.seek 0ml @20:39:31.70 Purging external Intake @20:39:31.86 CSV.dial :flush @20:39:32.38 CS.reconfigure CSfast @20:39:32.45 CS.seek 10ml @20:39:59.01 delay 5 seconds @20:40:04.04 CSV.dial CBV @20:40:04.09
CBV.dial CSV @20:40:04.95 CTV.dial :intake @20:40:05.82 Intake.select :open @20:40:05.96 CS.seek 0ml @20:40:33.29 Intake.select :close @20:40:33.42 CTV.dial :air @20:40:34.30 Flushing upper manifold @20:40:34.45 CSV.dial :flush @20:40:35.32 CS.seek 5ml @20:40:49.04 delay 5 seconds @20:40:54.07 CSV.dial :air @20:40:54.65 CS.seek 7ml @20:41:00.76 CSV.dial CBV @20:41:00.80
CBV.dial CSV @20:41:01.98 CTV.dial :air @20:41:02.01 CS.seek 0ml @20:41:20.70 delay 7 seconds @20:41:27.74 CS.seek 3ml @20:41:36.39 CSV.dial :waste @20:41:36.93 CS.seek 0ml @20:41:45.67 CSV.dial :flush @20:41:46.63 CS.seek 3ml @20:41:55.23 delay 5 seconds @20:42:00.26 CSV.dial :air @20:42:00.86 CS.seek 5ml @20:42:07.11 CSV.dial :air @20:42:07.14 CS.seek 0ml @20:42:20.76 delay 7 seconds @20:42:27.80 CS.seek 3ml @20:42:36.67 CSV.dial :waste @20:42:37.52 CS.seek 0ml @20:42:46.33 Garaging FlushPuck in Collection Clamp @20:42:46.43 Elbow.reconfigure TightElbow @20:42:46.50 Elbow.seek CC @20:42:46.80 Elbow.reconfigure LooseElbow @20:42:46.93 Forearm.seek CC @20:42:47.94 Hand.close @20:42:48.76 CC.seek :open @20:42:53.15 Forearm.seek :retract @20:42:54.23 Elbow.reconfigure TightElbow @20:42:54.30 Elbow.seek :garage @20:42:57.41 Elbow.reconfigure LooseElbow @20:42:57.54 Forearm.reconfigure TightForearm @20:42:57.61 Forearm.seek :garage @20:42:58.80 Hand.open @20:42:59.69 Forearm.reconfigure LooseForearm @20:42:59.77 Forearm.seek :retract @20:43:00.92 Starting mfb @20:43:00.93 pcrBAC @20:43:00.99 BACfans.email "Started mfb in tube 4 at 20:43:00CEST06-Sep-18\n{1 => 0, 2 => 0, 3 => 0, 4 => 2, 5 => 0, 6 => 0, 7 => 0}\nCan@20:38:23, 24.8C, 62% humidity, 14.7psia, 11.913V, 1.036A, 1.029A avg, 12.34W",Subject: "pcrBAC" @20:43:01.10
Loading sh1 puck from tube 4 into Collection Clamp @20:43:01.24 Readying the MFB @20:43:01.31 Homing MFB Syringe @20:43:01.33 AFV.select :holdingCoil @20:43:01.62 ATV.dial :air2 @20:43:01.65
SC.reconfigure SCtight @20:43:01.80 SC.seek 4 @20:43:03.22 AS.reconfigure ASconfig @20:43:03.29 AS.home.seek :home @20:43:05.44
SC.reconfigure SCloose @20:43:05.72 Elbow.reconfigure TightElbow @20:43:05.81 Elbow.seek 4 @20:43:08.00 Forearm.reconfigure TightForearm @20:43:08.08 Forearm.seek 4 + 700 counts @20:43:08.82 AFV.select :carrierSolution @20:43:08.95 delay 2 seconds @20:43:09.21
SE.reconfigure SEpick @20:43:09.33 SE.seek beamBreak @20:43:10.99 AS.seek 1ml @20:43:12.42
SE.configure SEconfig @20:43:12.64 Elbow.seek 4 @20:43:13.07 Forearm.seek 4 + 300 counts @20:43:13.37 Forearm.reconfigure LooseForearm @20:43:13.44 Hand.close @20:43:14.27 SE.seek :down @20:43:14.53
Forearm.seek :retract @20:43:15.51 Elbow.seek CC @20:43:17.21 SE.coast @20:43:17.37
Elbow.reconfigure LooseElbow @20:43:17.52 Forearm.reconfigure TightForearm @20:43:17.59 Forearm.seek CC @20:43:18.93 CC.seek :closed @20:43:23.25 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.36 @20:43:23.26 Hand.open @20:43:24.08 Forearm.reconfigure LooseForearm @20:43:24.15 Forearm.seek :retract @20:43:25.16 SC.coast @20:43:25.22 skipping prime and sample collection @20:43:25.24 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @20:43:25.27 Intake.select :close @20:43:25.38 CSV.dial :waste @20:43:25.48 CS.seek 0ml @20:43:25.59 CSV.dial :air @20:43:25.67
Calibrating pressure and temperature sensors @20:43:25.73 CTV.dial :air @20:43:25.75 SSV.dial :spit,avoiding: :exhaust @20:43:25.79 SS.reconfigure SSfast @20:43:25.89 SS.seek 5ml @20:43:26.52 CS.seek 10ml @20:43:29.10 SSV.dial CBV @20:43:29.13
CBV.dial SSV @20:43:30.66 delay 3.8 seconds @20:43:34.61 SSV.dial :spit @20:43:36.14 SS.seek 0ml @20:43:38.98 SSV.dial CBV @20:43:40.48 SSV.dial CBV,avoiding: :exhaust @20:43:40.52
CBV.dial SSV @20:43:40.55 CTV.dial :air @20:43:40.59 delay 15 seconds @20:43:43.21 -> can @20:43:55.62
SSV.dial :spit,avoiding: :exhaust @20:43:57.16 CTV.dial CSV,avoiding: :intake @20:43:57.20
SS.reconfigure SSexhale @20:43:57.34 SS.seek 0ml @20:43:57.51 SSV.dial CBV,avoiding: :exhaust @20:43:59.12 CBV.dial between SSV and waste2 @20:43:59.58 CTV.dial CSV,avoiding: :air @20:43:59.61
CSV.dial CTV @20:44:00.26 CS.reconfigure CSpressurize @20:44:00.34 CS.seek 0ml @20:44:03.91 delay 1 second @20:44:04.93 AFV.select :holdingCoil @20:44:05.03 delay 1 second @20:44:06.06 ATV.dial :waste @20:44:07.13 AS.reconfigure ASnorm @20:44:07.20 AS.home.seek :empty @20:44:26.48
CSV.dial :air @20:44:27.12 CS.reconfigure CSfast @20:44:27.20 CS.seek 10ml @20:44:53.61 CSV.dial between air and CTV @20:44:53.99 CS.seek 3.429ml @20:44:59.82 ATV.dial :waste @20:44:59.83 ARV.dial between carrier and air1 @20:44:59.95 ASV.dial :waste1 @20:45:11.58 CTV.dial CSV,avoiding: :air @20:45:11.62
CSV.dial CTV @20:45:11.98 CS.reconfigure CSpressurize @20:45:12.06 CS.seek 0ml @20:45:21.61 CSV.dial :air @20:45:22.16 CS.reconfigure CSfast @20:45:22.24 CS.seek 10ml @20:45:48.65 CSV.dial between air and CTV @20:45:49.53 CS.seek 2.166ml @20:46:10.62 CTV.dial CSV,avoiding: :air @20:46:10.65
CSV.dial CTV @20:46:11.02 CS.reconfigure CSpressurize @20:46:11.10 CS.seek 0ml @20:46:13.08 BubblePoint: Intake @69.9psia, Outlet @14.6psia @20:46:13.09 CBV.dial SSV @20:46:13.48 SS.reconfigure SSconfig @20:46:13.56 SS.seek 10ml @20:46:19.57 delay 15 seconds @20:46:34.73 Evacuated: Intake @28.5psia, Outlet @28.8psia @20:46:34.74 SSV.dial :spit,avoiding: :exhaust @20:46:36.28 SS.reconfigure SSexhale @20:46:36.36 SS.seek 0ml @20:46:41.23 SS.reconfigure SSfast @20:46:41.31 SS.seek 5ml @20:46:44.53 SSV.dial CBV,avoiding: :exhaust @20:46:44.56
CBV.dial SSV @20:46:46.12 CTV.dial :air,avoiding: :intake @20:46:47.65 CSV.dial CTV @20:46:47.70
SS.seek 10ml @20:46:48.69 CSV.dial :air @20:46:49.23 CS.reconfigure CSfast @20:46:49.31 CS.seek 0ml @20:46:50.71
SSV.dial :spit,avoiding: :exhaust @20:46:52.25 SS.reconfigure SSexhale @20:46:52.33 SS.seek 0ml @20:46:54.45 CS.coast @20:46:57.37
SS.reconfigure SSfast @20:46:57.46 SS.seek 5ml @20:47:00.44 SSV.dial CBV,avoiding: :exhaust @20:47:00.47
CBV.dial SSV @20:47:03.01 SSV.dial :spit,avoiding: :exhaust @20:47:04.53 SS.reconfigure SSconfig @20:47:04.61 SS.seek 0ml @20:47:07.93 SS.coast @20:47:08.00 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @20:47:08.01 Intake.select :close @20:47:08.14 CSV.dial :waste @20:47:08.29 SSV.dial CBV,avoiding: :exhaust @20:47:08.32
CBV.dial SSV @20:47:09.04 CS.seek 0ml @20:47:09.15 CSV.dial :air @20:47:09.84
CTV.dial :air @20:47:09.86 delay 15 seconds @20:47:09.99 CS.seek 10ml @20:47:15.97 -> exit @20:47:16.00 Session Ended @20:47:24.90
SSV.dial :spit,avoiding: :exhaust @20:47:26.41 CTV.dial CSV,avoiding: :intake @20:47:26.45
SS.reconfigure SSexhale @20:47:26.56 SS.seek 0ml @20:47:27.01 SSV.dial CBV,avoiding: :exhaust @20:47:36.46 CBV.dial between SSV and waste2 @20:47:36.89 CTV.dial CSV,avoiding: :air @20:47:36.92
CSV.dial CTV @20:47:37.40 CS.reconfigure CSpressurize @20:47:37.45 CS.seek 0ml @20:48:03.55 CSV.dial :air @20:48:04.07 CS.reconfigure CSfast @20:48:04.12 CS.seek 10ml @20:48:30.43 CSV.dial between air and CTV @20:48:30.80 CS.seek 3.403ml @20:48:48.43 CTV.dial CSV,avoiding: :air @20:48:48.46
CSV.dial CTV @20:48:48.79 CS.reconfigure CSpressurize @20:48:48.84 CS.seek 0ml @20:48:58.23 CSV.dial :air @20:48:58.74 CS.reconfigure CSfast @20:48:58.79 CS.seek 10ml @20:49:25.02 CSV.dial between air and CTV @20:49:25.86 CS.seek 2.161ml @20:49:46.65 CTV.dial CSV,avoiding: :air @20:49:46.68
CSV.dial CTV @20:49:47.13 CS.reconfigure CSpressurize @20:49:47.18 CS.seek 0ml @20:49:49.30 BubblePoint: Intake @70.6psia, Outlet @15.3psia @20:49:49.31 CBV.dial SSV @20:49:49.67 SS.reconfigure SSconfig @20:49:49.72 SS.seek 10ml @20:49:55.77 delay 15 seconds @20:50:10.92 Evacuated: Intake @28.8psia, Outlet @29.3psia @20:50:10.93 SSV.dial :spit,avoiding: :exhaust @20:50:12.42 SS.reconfigure SSexhale @20:50:12.47 SS.seek 0ml @20:50:17.67 SS.reconfigure SSfast @20:50:17.72 SS.seek 5ml @20:50:20.61 SSV.dial CBV,avoiding: :exhaust @20:50:20.64
CBV.dial SSV @20:50:22.14 CTV.dial :air,avoiding: :intake @20:50:23.65 CSV.dial CTV @20:50:23.69
SS.seek 10ml @20:50:24.68 CSV.dial :air @20:50:25.18 CS.reconfigure CSfast @20:50:25.23 CS.seek 0ml @20:50:26.46
SSV.dial :spit,avoiding: :exhaust @20:50:27.95 SS.reconfigure SSexhale @20:50:28.00 SS.seek 0ml @20:50:30.27 CS.coast @20:50:33.23
SS.reconfigure SSfast @20:50:33.29 SS.seek 5ml @20:50:36.22 SSV.dial CBV,avoiding: :exhaust @20:50:36.25
CBV.dial SSV @20:50:38.77 SSV.dial :spit,avoiding: :exhaust @20:50:40.28 SS.reconfigure SSconfig @20:50:40.33 SS.seek 0ml @20:50:43.60 SS.coast @20:50:43.64 Lysing: Adding 1.5ml lysis @20:50:43.65 CBV.dial between CSV and waste1,avoiding: :DBV @20:50:45.34 CTV.dial CSV @20:50:46.81 CSV.dial CSR @20:50:47.69 Pulling 1ml of CSR.lysis @20:50:47.70 CSR.select :lysis @20:50:47.82 CS.reconfigure CSconfig @20:50:47.87 CS.seek 1ml @20:50:51.48 delay 5 seconds @20:50:56.51 CSR.select :air @20:50:56.69 CS.seek 6.001ml @20:51:10.42 delay 2 seconds @20:51:12.44 CSV.dial :waste1 @20:51:13.90 CS.reconfigure CSfast @20:51:13.95 CS.seek :empty @20:51:29.92 CSV.dial CSR @20:51:31.43 Pulling 1.5ml of CSR.lysis @20:51:31.44 CSR.select :lysis @20:51:31.56 CS.reconfigure CSconfig @20:51:31.61 CS.seek 1.5ml @20:51:36.45 delay 5 seconds @20:51:41.48 CSR.select :air @20:51:41.66 CS.seek 4.5ml @20:51:50.33 delay 2 seconds @20:51:52.35 CSV.dial CTV @20:51:52.87 CS.seek 1.275ml @20:52:02.09 Heating sh1 puck from tube 4 in Collection Clamp to 85C for 5:00 @20:52:02.11 CH.seek 85C,8:00 @20:54:57.44 CH.hold 5:00,85C @20:59:57.62 Collection Puck Heater at 85.1C after lysing @20:59:57.64 CS.seek 0ml @21:00:01.87 CTV.dial between spare and air,avoiding: :intake @21:00:03.03 CSV.dial :air @21:00:03.53 CS.seek 5ml @21:00:17.27 CSV.dial CTV @21:00:17.79 delay 2 seconds @21:00:19.82 CSV.dial :air @21:00:20.33 delay 2 seconds @21:00:22.36 CSV.dial CTV @21:00:22.88 delay 2 seconds @21:00:24.91 CSV.dial :air @21:00:25.41 delay 2 seconds @21:00:27.44 CS.seek 0ml @21:00:41.18 Recovering Lysate into Collection syringe @21:00:41.19 CSV.dial CBV @21:00:42.32 CBV.dial CSV @21:00:42.76 CS.seek 5ml @21:00:56.90 delay 5 seconds @21:01:01.93 CTV.dial :air @21:01:02.51 CS.seek 7ml @21:01:08.61 delay 5 seconds @21:01:13.64 CSV.dial :air2 @21:01:14.13 CS.seek 10ml @21:01:22.74 CSV.dial between air and CTV,avoiding: CBV @21:01:24.05 Lysing Complete @21:01:24.13 Storing sh1 puck in Collection Clamp to tube 3 @21:01:24.32 SC.reconfigure SCtight @21:01:24.37 SC.seek 3 @21:01:26.54 SC.reconfigure SCloose @21:01:26.71 SE.reconfigure SEplace @21:01:26.79
Elbow.reconfigure TightElbow @21:01:26.85 Elbow.seek CC @21:01:26.95 SE.seek beamBreak @21:01:27.02
Elbow.reconfigure LooseElbow @21:01:27.12 Forearm.seek CC @21:01:28.11 Hand.close @21:01:28.90 CC.seek :open @21:01:29.90 SE.configure SEconfig @21:01:29.96 SE.seek :place @21:01:33.45
Forearm.seek :retract @21:01:34.45 Elbow.reconfigure TightElbow @21:01:34.50 Elbow.seek 3 @21:01:36.50 Elbow.reconfigure LimpElbow @21:01:36.56 Forearm.seek 3 @21:01:37.58 Elbow.hold @21:01:37.66 Elbow.reconfigure TightElbow @21:01:37.71 Hand.open @21:01:38.54 Forearm.force -20 @21:01:39.01 Forearm.seek 3 + 348 counts @21:01:39.43 Forearm.reconfigure TightForearm @21:01:39.48 Forearm.seek 3 + 700 counts @21:01:40.00 SE.seek :down @21:01:41.15
Forearm.reconfigure LooseForearm @21:01:41.20 Forearm.seek :retract @21:01:42.80 SE.coast @21:01:42.84
SC.coast @21:01:42.88 Tranferring lysate to PRV @21:01:42.89 CSV.dial :air @21:01:43.22 CS.seek 10ml @21:01:43.30 CSV.dial between air and CTV @21:01:43.78 AIV.dial :bypass @21:01:43.88 Beginning samplePassC @21:01:43.89 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:01:43.97 Hand.open @21:01:44.42 Elbow.seek FlushPuck::Garage @21:01:46.42 Elbow.reconfigure LooseElbow @21:01:46.54 Forearm.seek :garage @21:01:47.58 Hand.close @21:01:48.41 Forearm.seek :retract @21:01:49.49 Elbow.reconfigure TightElbow @21:01:49.53 Elbow.seek CC @21:01:52.43 Elbow.reconfigure LooseElbow @21:01:52.54 Forearm.reconfigure TightForearm @21:01:52.59 Forearm.seek CC @21:01:53.90 CC.seek :closed @21:01:58.16 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.36 @21:01:58.24 Hand.open @21:01:59.10 Forearm.reconfigure LooseForearm @21:01:59.15 Forearm.seek :retract @21:02:00.11 AWV.select :air @21:02:00.12 AIV.dial :bypass @21:02:00.14 PSV.dial PSR @21:02:01.68 Pulling 1.5ml of PSR.flush @21:02:01.69 PSR.select :flush @21:02:01.82 PS.seek 1.5ml @21:02:17.98 delay 5 seconds @21:02:23.00 PSV.dial :waste1 @21:02:23.52 PS.seek 0ml @21:02:39.82 PSV.dial PSR @21:02:40.33 PSR.select :air @21:02:40.46 PS.seek 2.5ml @21:03:07.02 PRV.dial CBV @21:03:07.05
CBV.dial PRV @21:03:07.94 PSV.dial :mixloop @21:03:09.10 CTV.dial :air,avoiding: :intake @21:03:09.12 PTV.dial :PRVmixing @21:03:09.20 PS.seek 0ml @21:03:35.63 PSV.dial :air @21:03:36.12 PS.seek 2.5ml @21:04:02.32 PSV.dial :mixloop @21:04:02.84 PS.seek 0ml @21:04:29.54 PSV.dial :air @21:04:30.06 PS.seek 2.5ml @21:04:56.51 PSV.dial :mixloop @21:04:57.02 PS.seek 0ml @21:05:23.69 Garaging FlushPuck in Collection Clamp @21:05:23.78 Elbow.reconfigure TightElbow @21:05:23.83 Elbow.seek CC @21:05:24.08 Elbow.reconfigure LooseElbow @21:05:24.19 Forearm.seek CC @21:05:25.15 Hand.close @21:05:25.91 CC.seek :open @21:05:30.32 Forearm.seek :retract @21:05:31.30 Elbow.reconfigure TightElbow @21:05:31.35 Elbow.seek :garage @21:05:34.44 Elbow.reconfigure LooseElbow @21:05:34.55 Forearm.reconfigure TightForearm @21:05:34.60 Forearm.seek :garage @21:05:35.76 Hand.open @21:05:36.60 Forearm.reconfigure LooseForearm @21:05:36.65 Forearm.seek :retract @21:05:37.73 Connecting PS to CS to transfer sample @21:05:37.74 CBV.dial AIV @21:05:37.76 AIV.dial :bypass @21:05:37.77 PSV.dial :mixloop @21:05:37.78 PTV.dial :PRVmixing @21:05:37.80 PRV.dial AIV @21:05:37.81 CTV.dial :air,avoiding: :intake @21:05:37.83 Loading lyfil puck from tube 4 into Collection Clamp @21:05:38.09 SC.reconfigure SCtight @21:05:38.15 SC.seek 4 @21:05:40.24 SC.reconfigure SCloose @21:05:40.48 Elbow.reconfigure TightElbow @21:05:40.53 Elbow.seek 4 @21:05:42.69 Forearm.reconfigure TightForearm @21:05:42.74 Forearm.seek 4 + 700 counts @21:05:43.79 SE.reconfigure SEpick @21:05:43.87 SE.seek beamBreak @21:05:46.91 SE.configure SEconfig @21:05:47.08 Elbow.seek 4 @21:05:47.48 Forearm.seek 4 + 300 counts @21:05:47.74 Forearm.reconfigure LooseForearm @21:05:47.79 Hand.close @21:05:48.53 Puck::Warning in MAIN -- Loaded Last remaining Puck (from Tube 4) @21:05:48.56 SE.seek :down @21:05:48.81
Forearm.seek :retract @21:05:49.77 Elbow.seek CC @21:05:51.55 SE.coast @21:05:51.58
Elbow.reconfigure LooseElbow @21:05:51.72 Forearm.reconfigure TightForearm @21:05:51.78 Forearm.seek CC @21:05:53.03 CC.seek :closed @21:05:57.30 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.46 @21:05:57.32 Hand.open @21:05:58.10 Forearm.reconfigure LooseForearm @21:05:58.15 Forearm.seek :retract @21:05:59.13 SC.coast @21:05:59.17 CTV.dial CSV,avoiding: :intake @21:06:00.65 CSV.dial CTV @21:06:01.02 CS.seek 5ml @21:06:14.77 delay 1:20 @21:07:34.81 CS.seek 2.5ml @21:07:42.15 delay 40 seconds @21:08:22.19 CS.seek 0ml @21:08:29.53 delay 1:00 @21:09:29.56 PSV.dial :air @21:09:30.08 PS.seek 2.5ml @21:09:56.44 PSV.dial :mixloop @21:09:56.94 delay 3 seconds @21:09:59.97 PSV.dial :air @21:10:00.49 delay 2 seconds @21:10:02.51 PSV.dial :mixloop @21:10:03.03 delay 3 seconds @21:10:06.05 PSV.dial :waste1 @21:10:07.66 PS.seek :empty @21:10:33.98 CBV.dial :waste1 @21:10:34.51 delay 5 seconds @21:10:39.54 CBV.dial AIV @21:10:40.07 delay 10 seconds @21:10:50.10 CBV.dial :waste1 @21:10:50.62 delay 10 seconds @21:11:00.65 CBV.dial AIV @21:11:01.17 delay 10 seconds @21:11:11.20 CTV.dial CSV,avoiding: :intake @21:11:11.22 CBV.dial between AIV and waste1 @21:11:11.60 CSV.dial :air @21:11:12.11 CS.seek 10ml @21:11:38.65 CSV.dial CTV @21:11:39.27 CS.seek 0ml @21:12:05.84 CBV.dial :waste1 @21:12:06.17 delay 10 seconds @21:12:16.20 CTV.dial :air,avoiding: :intake @21:12:17.77 Storing lyfil puck in Collection Clamp to tube 3 @21:12:17.97 SC.reconfigure SCtight @21:12:18.02 SC.seek 3 @21:12:20.14 SC.reconfigure SCloose @21:12:20.38 Elbow.reconfigure TightElbow @21:12:20.43 Elbow.seek 3 @21:12:22.37 Forearm.reconfigure TightForearm @21:12:22.42 Forearm.seek 3 + 700 counts @21:12:23.49 SE.reconfigure SEpick @21:12:23.57 SE.seek beamBreak @21:12:26.68 SE.configure SEconfig @21:12:26.85 Elbow.reconfigure LimpElbow @21:12:26.91 Forearm.reconfigure LooseForearm @21:12:26.95 Forearm.seek 3 @21:12:27.26 Elbow.hold @21:12:27.34 Elbow.reconfigure TightElbow @21:12:27.44 SE.seek 0.06 Pucks below Top Plate @21:12:27.91 Forearm.force -20 @21:12:27.95 Forearm.seek 3 + 341 counts @21:12:28.29 Forearm.reconfigure TightForearm @21:12:28.34 Forearm.seek 3 + 700 counts @21:12:28.76 SE.seek 1.06 Pucks below Top Plate @21:12:29.37 Forearm.reconfigure LooseForearm @21:12:29.43 Forearm.seek :retract @21:12:30.35 Elbow.seek CC @21:12:32.58 Elbow.reconfigure LooseElbow @21:12:32.69 Forearm.seek CC @21:12:33.66 Hand.close @21:12:34.42 CC.seek :open @21:12:38.98 Forearm.seek :retract @21:12:39.96 Elbow.reconfigure TightElbow @21:12:40.01 Elbow.seek 3 @21:12:41.98 Elbow.reconfigure LimpElbow @21:12:42.04 Forearm.seek 3 @21:12:43.08 Elbow.hold @21:12:43.16 Elbow.reconfigure TightElbow @21:12:43.21 Hand.open @21:12:44.06 Forearm.force -20 @21:12:44.10 Forearm.seek 3 + 343 counts @21:12:44.45 Forearm.reconfigure TightForearm @21:12:44.50 Forearm.seek 3 + 700 counts @21:12:44.80 SE.seek :down @21:12:45.97
Forearm.reconfigure LooseForearm @21:12:46.02 Forearm.seek :retract @21:12:47.56 SE.coast @21:12:47.60
SC.coast @21:12:47.64 PSV.dial :air @21:12:48.88 PS.seek 0.499ml @21:12:54.85 delay 3 seconds @21:12:57.87 PSV.dial :mixloop @21:12:58.34 PRV.dial :air @21:12:58.86 delay 3 seconds @21:13:01.88 PRV.dial CBV @21:13:02.38 CBV.dial AIV @21:13:02.90 PRV.dial between CBV and air @21:13:03.26 PRV.dial :air @21:13:03.68 PS.reconfigure PSslow @21:13:03.73 PS.seek 0.599ml @21:13:06.26 PRV.dial :waste2 @21:13:07.07 PS.reconfigure PSconfig @21:13:07.11 PS.seek :empty @21:13:14.11 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:13:14.18 Hand.open @21:13:14.55 Elbow.seek FlushPuck::Garage @21:13:16.60 Elbow.reconfigure LooseElbow @21:13:16.71 Forearm.seek :garage @21:13:17.77 Hand.close @21:13:18.68 Forearm.seek :retract @21:13:19.70 Elbow.reconfigure TightElbow @21:13:19.74 Elbow.seek CC @21:13:22.63 Elbow.reconfigure LooseElbow @21:13:22.74 Forearm.reconfigure TightForearm @21:13:22.79 Forearm.seek CC @21:13:24.61 CC.seek :closed @21:13:28.97 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.31 @21:13:29.05 Hand.open @21:13:29.86 Forearm.reconfigure LooseForearm @21:13:29.91 Forearm.seek :retract @21:13:30.99 Clearing PML for modified lysate @21:13:31.00 CTV.dial :air,avoiding: :intake @21:13:31.01 PRV.dial :waste2 @21:13:31.02 PTV.dial :PRV,avoiding: :puck @21:13:31.86 delay 2 seconds @21:13:33.87 PTV.dial :PRVmixing,avoiding: :puck @21:13:34.70 delay 2 seconds @21:13:36.73 PSV.dial :PML @21:13:36.75 delay 2 seconds @21:13:38.77 PRV.dial :air @21:13:39.60 delay 2 seconds @21:13:41.70 PS.seek 1.499ml @21:13:57.85 delay 5 seconds @21:14:02.89 PS.seek 2.5ml @21:14:13.82 PSV.dial :air @21:14:14.35 PS.seek 0ml @21:14:40.54 mixing lysate with speDil @21:14:40.55 PSV.dial :PML @21:14:41.05 CBV.dial :PRV @21:14:41.07 PRV.dial :air @21:14:41.08 delay 2 seconds @21:14:43.10 PRV.dial :speDil @21:14:43.63 delay 2 seconds @21:14:45.71 PS.reconfigure PSslow1 @21:14:45.77 PS.seek 0.225ml @21:15:09.95 delay 5 seconds @21:15:14.98 PRV.dial CBV @21:15:15.82 delay 2 seconds @21:15:17.92 PS.seek 0.475ml @21:15:43.76 delay 5 seconds @21:15:48.79 PRV.dial :air @21:15:49.32 delay 2 seconds @21:15:51.35 PS.seek 0.965ml @21:16:41.48 delay 10 seconds @21:16:51.51 PRV.dial CBV @21:16:52.04 CTV.dial :air,avoiding: :intake @21:16:52.06 PSV.dial :air @21:16:52.58 PS.reconfigure PSconfig @21:16:52.63 PS.seek 2.5ml @21:17:08.98 delay 3 seconds @21:17:12.00 PSV.dial PTV @21:17:12.84 PTV.dial :PRV,avoiding: :puck @21:17:13.68 PS.seek 0ml @21:17:39.88 PSV.dial :air @21:17:40.71 PS.seek 2.5ml @21:18:07.04 delay 3 seconds @21:18:10.06 PSV.dial PTV @21:18:10.88 PTV.dial :PRV,avoiding: :puck @21:18:10.91 PS.seek 0ml @21:18:37.07 Pushing modified lysate into AIV Injection Loop @21:18:37.08 CBV.dial AIV @21:18:37.10 AIV.dial :bypass @21:18:37.11 PRV.dial CBV @21:18:37.12 PSV.dial :air @21:18:37.96 PS.seek 2.5ml @21:19:04.29 delay 3 seconds @21:19:07.31 PSV.dial :PML @21:19:07.81 PTV.dial :PRVmixing,avoiding: :puck @21:19:08.71 PS.reconfigure PSslow1 @21:19:08.76 PS.seek 1.74ml @21:20:26.17 delay 5 seconds @21:20:31.20 AIV.dial :sample @21:20:31.83 AWV.select :waste @21:20:32.00 PS.reconfigure PSslow2 @21:20:32.06 PS.seek 1.22ml @21:22:20.64 delay 15 seconds @21:22:35.69 AIV.dial :bypass @21:22:36.32 PS.reconfigure PSconfig @21:22:36.37 PS.seek 0ml @21:22:49.51 PSV.dial :air @21:22:50.02 PS.seek 2.5ml @21:23:16.14 PSV.dial :PML @21:23:16.65 PS.seek 0ml @21:23:42.79 AIV.dial :coilToMFB @21:23:42.80 AWV.select :air @21:23:42.98 Extracting Nucleic Acid from AIV @21:23:43.01
Beginning samplePassD @21:23:43.03 SPE.hold 2:46:40,55C @21:23:43.14 ASV.dial :SPEdelivery @21:23:43.21
AWV.select :air @21:23:43.22 AIV.dial :bypass @21:23:43.24 PSV.dial PSR @21:23:43.54 delay 20 seconds @21:23:44.53
Pulling 1.5ml of PSR.killMFB @21:23:44.54 PSR.select :killMFB @21:23:44.68 PS.seek 1.5ml @21:24:01.55 delay 5 seconds @21:24:03.57 primeLysateINT @21:24:03.58 AWV.select :air @21:24:03.60 AIV.dial :coilToMFB @21:24:03.61 ATV.dial AIV @21:24:05.49 AS.reconfigure ASslow1 @21:24:05.54 AS.seek 0.299ml @21:24:06.57
PSV.dial :waste1 @21:24:07.11 PS.seek 0ml @21:24:23.95 PSV.dial PSR @21:24:24.49 PSR.select :air @21:24:24.62 PS.seek 2.5ml @21:24:52.18 PRV.dial CBV @21:24:52.21
CBV.dial PRV @21:24:52.23 PSV.dial :mixloop @21:24:53.42 CTV.dial :air,avoiding: :intake @21:24:53.44 PTV.dial :PRVmixing @21:24:53.46 PS.seek 0ml @21:25:06.26 delay 10 seconds @21:25:16.29 ATV.dial :air2 @21:25:17.21 AS.seek 0.329ml @21:25:21.17
PSV.dial PSR @21:25:22.56 Pulling 1.5ml of PSR.flush @21:25:22.57 PSR.select :flush @21:25:22.70 PS.seek 1.5ml @21:25:23.51 ATV.dial :waste2 @21:25:24.13 AS.reconfigure ASnorm @21:25:24.18 AS.seek :empty @21:25:39.52
delay 5 seconds @21:25:41.21 Lysate primed! @21:25:41.23 AFV.select :holdingCoil @21:25:41.24 ATV.dial :air2 @21:25:41.91 AS.reconfigure ASslow1 @21:25:41.96 AS.seek 0.069ml @21:25:44.54
PSV.dial :waste1 @21:25:45.10 PS.seek 0ml @21:25:56.33 delay 3 seconds @21:25:59.36 ATV.dial :AIV @21:26:00.26 AS.seek 0.469ml @21:26:02.11
PSV.dial PSR @21:26:02.65 PSR.select :air @21:26:02.78 PS.seek 2.5ml @21:26:30.23 PRV.dial CBV @21:26:30.26
CBV.dial PRV @21:26:30.28 PSV.dial :mixloop @21:26:31.47 CTV.dial :air,avoiding: :intake @21:26:31.48 PTV.dial :PRVmixing @21:26:31.50 PS.seek 0ml @21:26:58.67 PSV.dial PSR @21:26:59.94 Pulling 1.5ml of PSR.flush @21:26:59.95 PSR.select :flush @21:27:00.08 PS.seek 1.5ml @21:27:16.58 delay 5 seconds @21:27:20.98 delay 5 seconds @21:27:21.59
PSV.dial :waste1 @21:27:22.12 PS.seek 0ml @21:27:26.05 Solid Phase Extraction Heater at 50.2C @21:27:26.06 ATV.dial ASV @21:27:26.99 ASV.dial :SPEdelivery @21:27:27.09 AS.seek 0.041ml @21:27:38.76
PSV.dial PSR @21:27:39.29 PSR.select :air @21:27:39.44 PS.seek 2.5ml @21:28:06.33 PRV.dial CBV @21:28:06.35
CBV.dial PRV @21:28:06.37 PSV.dial :mixloop @21:28:07.53 CTV.dial :air,avoiding: :intake @21:28:07.55 PTV.dial :PRVmixing @21:28:07.57 PS.seek 0ml @21:28:34.42 PSV.dial PSR @21:28:35.66 Pulling 1.5ml of PSR.flush @21:28:35.67 PSR.select :flush @21:28:35.80 PS.seek 1.5ml @21:28:52.12 delay 5 seconds @21:28:53.46 delay 10 seconds @21:28:57.14
PSV.dial :waste1 @21:28:57.66 PS.seek 0ml @21:29:03.49 rinseLysateLineINT @21:29:03.50 AFV.select :carrierSolution @21:29:03.61 delay 2 seconds @21:29:05.65 AS.reconfigure ASconfig @21:29:05.70 AS.seek 1ml @21:29:14.07
PSV.dial PSR @21:29:14.59 PSR.select :air @21:29:14.72 PS.seek 2.5ml @21:29:41.51 PRV.dial CBV @21:29:41.54
CBV.dial PRV @21:29:41.56 PSV.dial :mixloop @21:29:42.73 CTV.dial :air,avoiding: :intake @21:29:42.75 PTV.dial :PRVmixing @21:29:42.77 PS.reconfigure PSslow @21:29:42.82 PS.seek 1.2ml @21:30:01.10 delay 1 second @21:30:02.13 AFV.select :holdingCoil @21:30:02.23 delay 1 second @21:30:03.26 ATV.dial AIV @21:30:03.93 AIV.dial :coilToMFB @21:30:03.95 AWV.select :waste @21:30:04.07 AS.reconfigure ASslow0 @21:30:04.12 AS.seek 0ml @21:30:09.61
PSV.dial :waste1 @21:30:11.12 PS.reconfigure PSconfig @21:30:11.17 PS.seek 0ml @21:30:24.23 PSV.dial :air @21:30:25.41 PS.seek 2.5ml @21:30:51.98 PSV.dial :mixloop @21:30:52.49 PS.reconfigure PSslow @21:30:52.54 PS.seek 0ml @21:31:43.67 PSV.dial :air @21:31:44.19 PS.reconfigure PSconfig @21:31:44.24 PS.seek 2.5ml @21:31:44.99 ATV.dial :air2 @21:31:45.69 AS.reconfigure ASslow1 @21:31:45.75 AS.seek 0.05ml @21:31:56.15 ATV.dial :AIV @21:31:56.99 AS.seek 0.01ml @21:32:05.32 ATV.dial :waste @21:32:06.98 AS.seek :empty @21:32:09.43 ethanol wash @21:32:09.45 ATV.dial :air2 @21:32:10.72 AS.reconfigure ASslow2 @21:32:10.80 AS.seek 0.009ml @21:32:10.83
PSV.dial :mixloop @21:32:11.41 PS.reconfigure PSslow @21:32:11.46 PS.seek 0ml @21:32:21.27 delay 3 seconds @21:32:24.29 Pulling 0.4ml of Ethanol Wash @21:32:24.30 ATV.dial :ethanol @21:32:25.12 AS.reconfigure ASslow1 @21:32:25.18 AS.seek 0.409ml @21:33:02.61
Cleaning Collection Syringe @21:33:02.63 CSV.dial CSR @21:33:03.31 Pulling 4ml of CSR.flush @21:33:03.32 CSR.select :flush @21:33:03.45 CS.seek 4ml @21:33:15.08 delay 5 seconds @21:33:20.10 CSR.select :air @21:33:20.23 CS.seek 10ml @21:33:36.60 CSV.dial CTV @21:33:36.64
CTV.dial CSV @21:33:38.69 CBV.dial :waste1 @21:33:39.22 CS.seek 0ml @21:33:45.94 delay 3 seconds @21:33:48.97 ATV.dial :air1 @21:33:49.38 delay 3 seconds @21:33:52.46 AS.seek 0.434ml @21:33:57.77 delay 3 seconds @21:34:00.80 ATV.dial :ethanol @21:34:01.43 AS.seek 0.419ml @21:34:04.72 delay 3 seconds @21:34:05.76
CSV.dial CSR @21:34:06.33 Pulling 4ml of CSR.flush @21:34:06.34 CSR.select :flush @21:34:06.47 CS.seek 4ml @21:34:07.75 ATV.dial ASV @21:34:09.10 AS.seek 0.092ml @21:34:17.74
delay 5 seconds @21:34:22.76 CSR.select :air @21:34:22.89 CS.seek 10ml @21:34:39.21 CSV.dial CTV @21:34:39.24
CTV.dial CSV @21:34:39.77 CBV.dial :waste1 @21:34:39.79 CS.seek 0ml @21:35:06.33 Garaging FlushPuck in Collection Clamp @21:35:06.41 Elbow.reconfigure TightElbow @21:35:06.46 Elbow.seek CC @21:35:06.73 Elbow.reconfigure LooseElbow @21:35:06.84 Forearm.seek CC @21:35:07.81 Hand.close @21:35:08.57 CC.seek :open @21:35:12.93 Forearm.seek :retract @21:35:13.91 Elbow.reconfigure TightElbow @21:35:13.95 Elbow.seek :garage @21:35:15.12 delay 3 seconds @21:35:17.14
Elbow.reconfigure LooseElbow @21:35:17.25 Forearm.reconfigure TightForearm @21:35:17.30 Forearm.seek :garage @21:35:18.14 ATV.dial :waste2 @21:35:18.46
Hand.open @21:35:18.92 AS.reconfigure ASconfig @21:35:18.97 AS.seek :empty @21:35:19.26
Forearm.reconfigure LooseForearm @21:35:19.31 Forearm.seek :retract @21:35:20.35 Skipping Whole Cell Archive @21:35:20.37 Starting finalPurge @21:35:20.38 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:35:20.46 Hand.open @21:35:20.76 Elbow.reconfigure TightElbow @21:35:20.81 Elbow.seek FlushPuck::Garage @21:35:20.95 Elbow.reconfigure LooseElbow @21:35:21.06 Forearm.seek :garage @21:35:22.10 Hand.close @21:35:22.95 Forearm.seek :retract @21:35:24.00 Elbow.reconfigure TightElbow @21:35:24.05 Elbow.seek CC @21:35:24.63 ATV.dial :air2 @21:35:25.05 AS.reconfigure ASslow1 @21:35:25.10 AS.seek 1ml @21:35:26.95
Elbow.reconfigure LooseElbow @21:35:27.06 Forearm.reconfigure TightForearm @21:35:27.11 Forearm.seek CC @21:35:28.88 CC.seek :closed @21:35:33.14 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.33 @21:35:33.22 Hand.open @21:35:34.01 Forearm.reconfigure LooseForearm @21:35:34.06 Forearm.seek :retract @21:35:35.04 Flushing the Collection Valve::Manifold @21:35:35.19 CSV.dial CSR @21:35:35.77 Pulling 5ml of CSR.flush @21:35:35.78 CSR.select :flush @21:35:35.91 CS.seek 5ml @21:35:49.61 delay 5 seconds @21:35:54.63 CSR.select :air @21:35:54.80 CS.reconfigure CSfast @21:35:54.86 CS.seek 7.5ml @21:36:02.29 CSV.dial :waste @21:36:03.75 CS.reconfigure CSconfig @21:36:03.80 CS.seek 0ml @21:36:24.04 Flushing lower manifold's waste1 port @21:36:24.19 CSV.dial :flush @21:36:24.69 CS.reconfigure CSfast @21:36:24.74 CS.seek 5ml @21:36:38.35 delay 5 seconds @21:36:43.38 CSV.dial :air @21:36:44.07 CS.seek 7ml @21:36:50.17 CSV.dial CTV @21:36:50.21
CTV.dial CSV @21:36:50.73 CBV.dial :waste1 @21:36:50.76 CS.seek 0ml @21:37:09.64 Flushing lower manifold's waste2b port @21:37:09.78 CSV.dial :flush @21:37:10.61 CS.seek 5ml @21:37:24.20 delay 5 seconds @21:37:29.23 CSV.dial :air @21:37:29.82 CS.seek 7ml @21:37:35.82 CSV.dial CTV @21:37:35.86
CTV.dial CSV @21:37:36.34 CBV.dial :waste2b @21:37:37.19 CS.seek 0ml @21:37:55.86 Flushing lower manifold's waste1 port @21:37:55.87 CSV.dial CSR @21:37:56.53 Pulling 4ml of CSR.mfbkill @21:37:56.54 CSR.select :mfbkill @21:37:56.66 CS.reconfigure CSconfig @21:37:56.71 CS.seek 4ml @21:38:07.89 delay 5 seconds @21:38:12.91 CSR.select :air @21:38:13.04 CS.reconfigure CSfast @21:38:13.09 CS.seek 10ml @21:38:29.33 CSV.dial CTV @21:38:29.36
CTV.dial CSV @21:38:29.85 CBV.dial :waste1 @21:38:30.71 CS.seek 0ml @21:38:46.66 delay 5 seconds @21:38:51.69 ATV.dial ASV @21:38:53.08 ASV.dial :SPEdelivery @21:38:53.11 AS.seek 0.02ml @21:38:57.89
Flushing lower manifold's waste2b port @21:38:57.91 CSV.dial CSR @21:38:58.47 Pulling 4ml of CSR.mfbkill @21:38:58.48 CSR.select :mfbkill @21:38:58.60 CS.reconfigure CSconfig @21:38:58.65 CS.seek 4ml @21:39:09.83 delay 5 seconds @21:39:14.85 CSR.select :air @21:39:14.98 CS.reconfigure CSfast @21:39:15.03 CS.seek 10ml @21:39:31.21 CSV.dial CTV @21:39:31.78
CTV.dial CSV @21:39:32.30 CBV.dial :waste2b @21:39:33.16 CS.seek 0ml @21:39:59.36 Killing upper manifold @21:39:59.37 CSV.dial CSR @21:39:59.94 Pulling 4ml of CSR.mfbkill @21:39:59.95 CSR.select :mfbkill @21:40:00.07 CS.reconfigure CSconfig @21:40:00.12 CS.seek 4ml @21:40:11.46 delay 5 seconds @21:40:16.49 CSR.select :air @21:40:16.62 CS.reconfigure CSfast @21:40:16.67 CS.seek 10ml @21:40:32.77 CSV.dial CBV @21:40:32.80
CBV.dial CSV @21:40:33.98 CTV.dial :air @21:40:35.47 CS.seek 0ml @21:41:01.73 delay 7 seconds @21:41:08.77 CS.seek 3ml @21:41:17.57 CSV.dial :waste @21:41:18.05 CS.seek 0ml @21:41:26.54 Flushing upper manifold @21:41:26.69 CSV.dial :flush @21:41:27.19 CS.seek 5ml @21:41:40.82 delay 5 seconds @21:41:45.85 CSV.dial :air @21:41:46.44 CS.seek 7ml @21:41:52.45 CSV.dial CBV @21:41:52.49
CBV.dial CSV @21:41:53.65 CTV.dial :air @21:41:53.67 CS.seek 0ml @21:42:10.49 delay 5 seconds @21:42:12.29
delay 7 seconds @21:42:15.52 ATV.dial :air2 @21:42:16.59 AS.seek 0.52ml @21:42:19.32
CS.seek 3ml @21:42:27.89 CSV.dial :waste @21:42:28.52 CS.seek 0ml @21:42:37.25 CSV.dial :flush @21:42:37.76 CS.seek 3ml @21:42:46.57 delay 5 seconds @21:42:51.60 CSV.dial :air @21:42:52.29 CS.seek 5ml @21:42:58.32 CSV.dial :air @21:42:58.34 CS.seek 0ml @21:43:11.92 delay 7 seconds @21:43:18.96 CS.seek 3ml @21:43:27.48 CSV.dial :waste @21:43:28.29 CS.seek 0ml @21:43:36.86 Flushing lower manifold's waste1 port @21:43:37.01 CSV.dial :flush @21:43:37.52 CS.seek 5ml @21:43:51.36 delay 5 seconds @21:43:56.39 CSV.dial :air @21:43:56.95 CS.seek 7ml @21:43:57.35 delay 5 seconds @21:44:02.38 ATV.dial ASV @21:44:02.98 CSV.dial CTV @21:44:03.02
CTV.dial CSV @21:44:03.35 ASV.dial :wasteR @21:44:03.83 AS.seek 0.3ml @21:44:04.58
CBV.dial :waste1 @21:44:05.10 CS.seek 0ml @21:44:23.73 Flushing lower manifold's waste2b port @21:44:23.88 CSV.dial :flush @21:44:24.71 CS.seek 5ml @21:44:38.42 delay 5 seconds @21:44:43.45 CSV.dial :air @21:44:44.05 CS.seek 7.001ml @21:44:48.38 delay 20 seconds @21:44:50.86 CSV.dial CTV @21:44:50.89
CTV.dial CSV @21:44:51.38 CBV.dial :waste2b @21:44:52.24 CS.seek 0ml @21:45:08.42 AS.seek 0.07ml @21:45:10.84
Garaging FlushPuck in Collection Clamp @21:45:10.93 Elbow.reconfigure TightElbow @21:45:10.98 Elbow.seek CC @21:45:11.24 Elbow.reconfigure LooseElbow @21:45:11.35 Forearm.seek CC @21:45:12.33 Hand.close @21:45:13.08 CC.seek :open @21:45:17.50 Forearm.seek :retract @21:45:18.53 Elbow.reconfigure TightElbow @21:45:18.58 Elbow.seek :garage @21:45:21.76 Elbow.reconfigure LooseElbow @21:45:21.88 Forearm.reconfigure TightForearm @21:45:21.93 Forearm.seek :garage @21:45:23.11 Hand.open @21:45:23.90 Forearm.reconfigure LooseForearm @21:45:23.95 Forearm.seek :retract @21:45:25.00 Starting initialPurge @21:45:25.02 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:45:25.09 Hand.open @21:45:25.40 Elbow.reconfigure TightElbow @21:45:25.45 Elbow.seek FlushPuck::Garage @21:45:25.59 Elbow.reconfigure LooseElbow @21:45:25.70 Forearm.seek :garage @21:45:26.72 Hand.close @21:45:27.58 Forearm.seek :retract @21:45:28.63 Elbow.reconfigure TightElbow @21:45:28.67 Elbow.seek CC @21:45:31.65 Elbow.reconfigure LooseElbow @21:45:31.76 Forearm.reconfigure TightForearm @21:45:31.81 Forearm.seek CC @21:45:33.16 CC.seek :closed @21:45:37.42 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.30 @21:45:37.51 Hand.open @21:45:38.26 Forearm.reconfigure LooseForearm @21:45:38.31 Forearm.seek :retract @21:45:39.32 Flushing the Collection Valve::Manifold @21:45:39.47 CSV.dial CSR @21:45:40.04 Pulling 5ml of CSR.flush @21:45:40.05 CSR.select :flush @21:45:40.17 CS.reconfigure CSconfig @21:45:40.22 CS.seek 5ml @21:45:54.01 delay 5 seconds @21:45:54.94 delay 20 seconds @21:45:59.04
CSR.select :air @21:45:59.22 CS.reconfigure CSfast @21:45:59.27 CS.seek 7.5ml @21:46:06.64 CSV.dial :waste @21:46:08.10 CS.reconfigure CSconfig @21:46:08.15 CS.seek 0ml @21:46:14.97 ATV.dial :waste2 @21:46:15.75 AS.reconfigure ASconfig @21:46:15.80 AS.seek :empty @21:46:20.19 Elution @21:46:20.20 ATV.dial :air1 @21:46:21.87 AS.reconfigure ASslow1 @21:46:21.93 AS.seek 0.049ml @21:46:28.36
Purging external Intake @21:46:28.51 CSV.dial :flush @21:46:29.01 CS.reconfigure CSfast @21:46:29.06 CS.seek 10ml @21:46:32.33 delay 3 seconds @21:46:35.36 ATV.dial :ElutionWater @21:46:36.05 AS.seek 0.109ml @21:46:48.36 delay 3 seconds @21:46:51.39 ATV.dial ASV,via: :air2 @21:46:53.60 ASV.dial :SPEelution @21:46:54.19 AFV.select :carrierSolution @21:46:54.36 AS.reconfigure ASnorm @21:46:54.41 AS.seek 0.759ml @21:46:55.26
delay 5 seconds @21:47:00.29 CSV.dial CBV @21:47:00.33
CBV.dial CSV @21:47:01.19 CTV.dial :intake @21:47:02.03 Intake.select :open @21:47:02.15 CS.seek 0ml @21:47:27.47 delay 3 seconds @21:47:29.55
Intake.select :close @21:47:29.66 CTV.dial :air @21:47:30.51 Flushing upper manifold @21:47:30.56 AFV.select :holdingCoil @21:47:30.69
CSV.dial :flush @21:47:30.80 AS.reconfigure ASslow1 @21:47:30.86 AS.seek 0.474ml @21:47:31.51
CS.seek 5ml @21:47:45.11 delay 5 seconds @21:47:50.14 CSV.dial :air @21:47:50.72 CS.seek 7ml @21:47:56.76 CSV.dial CBV @21:47:56.79
CBV.dial CSV @21:47:57.95 CTV.dial :air @21:47:57.98 CS.seek 0ml @21:48:16.86 delay 7 seconds @21:48:23.90 CS.seek 3ml @21:48:28.43 delay 5 seconds @21:48:32.36
CSV.dial :waste @21:48:32.99 CS.seek 0ml @21:48:33.51 AS.reconfigure ASslow2 @21:48:33.57 AS.seek 0.439ml @21:48:41.61
CSV.dial :flush @21:48:42.13 CS.seek 3ml @21:48:50.70 delay 5 seconds @21:48:55.73 CSV.dial :air @21:48:56.42 CS.seek 5ml @21:49:02.39 CSV.dial :air @21:49:02.42 CS.seek 0ml @21:49:08.97 delay 1:00 @21:49:16.17
delay 7 seconds @21:49:23.21 CS.seek 3ml @21:49:31.98 CSV.dial :waste @21:49:32.79 CS.seek 0ml @21:49:41.36 Garaging FlushPuck in Collection Clamp @21:49:41.45 Elbow.reconfigure TightElbow @21:49:41.50 Elbow.seek CC @21:49:41.73 Elbow.reconfigure LooseElbow @21:49:41.84 Forearm.seek CC @21:49:42.82 Hand.close @21:49:43.58 CC.seek :open @21:49:47.97 Forearm.seek :retract @21:49:48.96 Elbow.reconfigure TightElbow @21:49:49.00 Elbow.seek :garage @21:49:52.19 Elbow.reconfigure LooseElbow @21:49:52.30 Forearm.reconfigure TightForearm @21:49:52.35 Forearm.seek :garage @21:49:53.66 Hand.open @21:49:54.53 Forearm.reconfigure LooseForearm @21:49:54.58 Forearm.seek :retract @21:49:55.60 Completed [coreNEG] begun 20:38:23 @21:49:55.62 Extending 18tun until completion of MFBPCR @21:50:09.00 pushing eluate to holding coil @21:50:09.08 AS.seek 0.279ml @21:52:50.00 delay 30 seconds @21:53:20.53 ATV.dial :waste,via: :air3 @21:53:22.31 AS.reconfigure ASconfig @21:53:22.36 AS.seek :empty @21:53:38.75 Parking Bleach in LysateSource line and SPEcolumn @21:53:38.77 ATV.dial :air3 @21:53:39.62 AS.reconfigure ASslow1 @21:53:39.67 AS.seek 0.029ml @21:53:46.11 ATV.dial :ElutionWater @21:53:46.72 AS.seek 0.059ml @21:53:53.08 ATV.dial :air3 @21:53:53.71 AS.seek 0.069ml @21:53:55.97 delay 2 seconds @21:53:58.00 ATV.dial :bleach @21:53:58.51 AS.seek 0.769ml @21:56:19.61 delay 3 seconds @21:56:22.64 ATV.dial :air3 @21:56:23.20 delay 3 seconds @21:56:26.28 AS.seek 0.794ml @21:56:31.55 delay 3 seconds @21:56:34.58 ATV.dial :bleach @21:56:35.22 AS.seek 0.779ml @21:56:38.52 delay 3 seconds @21:56:41.55 ATV.dial :AIV @21:56:42.81 AS.reconfigure ASconfig @21:56:42.86 AS.seek 0.015ml @21:57:27.08 ATV.dial :waste @21:57:28.58 AS.seek :empty @21:57:29.82 ATV.dial :air3 @21:57:30.51 AS.reconfigure ASslow1 @21:57:30.56 AS.seek 0.009ml @21:57:32.91 delay 2 seconds @21:57:34.93 ATV.dial :bleach @21:57:35.41 AS.seek 0.579ml @21:59:30.30 delay 3 seconds @21:59:33.33 ATV.dial :air3 @21:59:33.88 delay 3 seconds @21:59:36.97 AS.seek 0.604ml @21:59:42.28 delay 3 seconds @21:59:45.31 ATV.dial :bleach @21:59:45.93 AS.seek 0.589ml @21:59:49.24 delay 3 seconds @21:59:52.26 ATV.dial :ASV @21:59:53.30 ASV.dial :wasteR @21:59:53.89 AS.seek 0ml @22:01:52.64 delay 1:00 @22:02:52.67 AFV.select :carrierSolution @22:02:52.77 delay 2 seconds @22:02:54.80 AS.reconfigure ASconfig @22:02:54.85 AS.seek 1ml @22:03:52.63 delay 1 second @22:03:53.65 AFV.select :holdingCoil @22:03:53.75 delay 1 second @22:03:54.78 ATV.dial ASV @22:03:54.79 ASV.dial :wasteR @22:03:54.81 AS.reconfigure ASslow1 @22:03:54.86 AS.seek 0ml @22:07:16.25 delay 20 seconds @22:07:36.28 ATV.dial :air3 @22:07:37.80 AS.seek 0.59ml @22:09:36.69 delay 3 seconds @22:09:39.72 ATV.dial :ASV @22:09:41.13 ASV.dial :wasteR @22:09:41.22 AS.seek 0.09ml @22:11:22.03 delay 30 seconds @22:11:52.06 ATV.dial :extSrc @22:11:52.93 delay 3 seconds @22:11:55.96 ATV.dial :ElutionWater @22:11:57.11 AS.seek 0.12ml @22:12:03.52 ATV.dial :air3 @22:12:04.15 AS.seek 0.13ml @22:12:06.36 delay 2 seconds @22:12:08.38 ATV.dial :bleach @22:12:08.85 AS.seek 0.23ml @22:12:29.25 delay 3 seconds @22:12:32.28 ATV.dial :air3 @22:12:32.83 delay 3 seconds @22:12:35.92 AS.seek 0.255ml @22:12:41.14 delay 3 seconds @22:12:44.17 ATV.dial :bleach @22:12:44.82 AS.seek 0.24ml @22:12:48.10 delay 3 seconds @22:12:51.12 ATV.dial :ASV @22:12:52.16 ASV.dial :SPEdelivery @22:12:52.55 AFV.select :carrierSolution @22:12:52.66 delay 2 seconds @22:12:54.70 AS.reconfigure ASconfig @22:12:54.74 AS.seek 0.5ml @22:13:10.50 delay 1 second @22:13:11.52 AFV.select :holdingCoil @22:13:11.68 AS.reconfigure ASslow1 @22:13:11.73 AS.seek 0.17ml @22:14:18.38 SPE.hold 2:46:40,95C @22:14:18.42 delay 3:00 @22:17:18.45 AFV.select :carrierSolution @22:17:18.55 delay 2 seconds @22:17:20.59 AS.reconfigure ASconfig @22:17:20.64 AS.seek 1ml @22:18:08.63 delay 1 second @22:18:09.65 AFV.select :holdingCoil @22:18:09.75 delay 1 second @22:18:10.78 ASV.dial :wasteD @22:18:11.87 AS.seek 0ml @22:19:09.66 Priming Eluate @22:19:09.67 AFV.select :carrierSolution @22:19:09.78 delay 2 seconds @22:19:11.82 AS.seek 0.5ml @22:19:40.88 delay 1 second @22:19:41.91 AFV.select :holdingCoil @22:19:42.01 ATV.dial ASV @22:19:42.03 ASV.dial :SPErecovery @22:19:42.64 Swish #1 of 2 @22:19:42.73 AS.reconfigure ASslow0 @22:19:42.78 AS.seek 0.6ml @22:19:53.18 AS.seek 0.5ml @22:20:03.56 Swish #2 of 2 @22:20:03.64 AS.seek 0.6ml @22:20:14.07 AS.seek 0.5ml @22:20:24.42 delay 3 seconds @22:20:27.51 AS.reconfigure ASslow1 @22:20:27.57 AS.seek 0.684ml @22:21:04.91 delay 3 seconds @22:21:07.94 Eluate primed @22:21:07.95 ATV.dial :waste2 @22:21:08.59 AS.reconfigure ASnorm @22:21:08.64 AS.seek 0ml @22:21:43.46 SPE.coast @22:21:43.54 ASV.dial :SPErecovery @22:21:43.57 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep0622h.pcr @22:21:43.58 Building PCR Slug from ASV with MM2 mix, PP4 primer @22:21:43.60 Priming with carrier @22:21:43.61 ATV.dial ARV @22:21:44.56 ARV.dial :carrier @22:21:44.91 AS.seek 0.5ml @22:22:10.47 AFV.select :carrierSolution @22:22:10.58 delay 2 seconds @22:22:12.62 AS.reconfigure ASconfig @22:22:12.66 AS.seek 1ml @22:22:41.69 delay 1 second @22:22:42.72 AFV.select :holdingCoil @22:22:42.82 delay 1 second @22:22:43.85 ATV.dial :waste @22:22:45.36 AS.reconfigure ASnorm @22:22:45.41 AS.seek :empty @22:23:36.14 AFV.select :carrierSolution @22:23:36.25 delay 2 seconds @22:23:38.29 AS.reconfigure ASconfig @22:23:38.33 AS.seek 0.5ml @22:24:07.44 delay 1 second @22:24:08.47 AFV.select :holdingCoil @22:24:08.58 Building Slug @22:24:08.59 ATV.dial :ARV @22:24:10.20 delay 2 seconds @22:24:12.22 ARV.dial :air1 @22:24:12.62 delay 2 seconds @22:24:14.70 AS.reconfigure ASslow2 @22:24:14.76 AS.seek 0.506ml @22:24:21.11 delay 2 seconds @22:24:23.14 ARV.dial :MM2 @22:24:24.66 delay 2 seconds @22:24:26.70 Adding MM2 @22:24:26.77 AS.seek 0.524ml @22:24:45.05 delay 25 seconds @22:25:10.08 ARV.dial :PP4 @22:25:10.48 Adding PP4 @22:25:10.49 delay 2 seconds @22:25:12.58 AS.seek 0.53ml @22:25:19.08 delay 10 seconds @22:25:29.11 ARV.dial :air3 @22:25:29.51 adding ARV air @22:25:29.52 delay 2 seconds @22:25:31.60 AS.seek 0.536ml @22:25:38.03 delay 5 seconds @22:25:43.06 ARV.dial :carrier @22:25:44.37 delay 7 seconds @22:25:51.46 AS.seek 0.594ml @22:26:50.06 delay 5 seconds @22:26:55.09 ATV.dial :ASV @22:26:55.60 delay 2 seconds @22:26:57.70 AS.seek 0.6ml @22:27:04.05 delay 4 seconds @22:27:08.08 ATV.dial ARV @22:27:08.71 delay 2 seconds @22:27:10.74 ARV.dial :carrier @22:27:10.76 delay 2 seconds @22:27:13.38 AS.seek 0.65ml @22:28:04.02 delay 4 seconds @22:28:08.05 ATV.dial PCR @22:28:08.67 ASV.dial :waste1 @22:28:09.47 ARV.dial :air1 @22:28:09.98 Positioning Slug by 0.324ml @22:28:10.07 AS.seek 0.326ml @22:33:36.08 delay 5 seconds @22:33:41.11 ATV.dial ARV @22:33:41.99 ASV.dial :PCRlock @22:33:42.37 delay 5 seconds @22:33:47.40 Running Tt1 Profile60C300 thermocycling @22:33:47.41 PCR.program Profile60C300 @22:33:47.54 Profile60C300 should complete in < 1:45:21 @22:33:47.56 PCR.run 2:16:41.25 @23:58:48.16 PCR.off @23:58:49.73 PCR for Tt1 completed -- closed /var/log/esp/Tt118sep0622h.pcr @23:58:49.81 Cleaning PCR @23:58:49.82 AFV.select :carrierSolution @23:58:49.93 delay 2 seconds @23:58:51.97 AS.reconfigure ASconfig @23:58:52.01 AS.seek 0.5ml @23:59:02.31 delay 1 second @23:59:03.33 AFV.select :holdingCoil @23:59:03.43 ASV.dial :waste1 @23:59:03.81 ATV.dial :air3 @23:59:05.10 AS.reconfigure ASslow1 @23:59:05.16 AS.seek 0.51ml @23:59:07.46 delay 2 seconds @23:59:09.48 ATV.dial :bleach @23:59:10.11 AS.seek 0.81ml @00:00:10.79CEST07-Sep-18 delay 3 seconds @00:00:13.82 ATV.dial :air3 @00:00:14.36 delay 3 seconds @00:00:17.46 AS.seek 0.835ml @00:00:22.78 delay 3 seconds @00:00:25.81 ATV.dial :bleach @00:00:26.44 AS.seek 0.82ml @00:00:29.75 delay 3 seconds @00:00:32.78 ARV.dial :waste @00:00:33.39 delay 3 seconds @00:00:36.42 ATV.dial :ARV @00:00:37.32 AS.reconfigure ASconfig @00:00:37.38 AS.seek 0.47ml @00:00:57.79 AFV.select :carrierSolution @00:00:57.90 delay 2 seconds @00:00:59.94 AS.seek 1ml @00:01:30.73 delay 1 second @00:01:31.75 AFV.select :holdingCoil @00:01:31.85 delay 1 second @00:01:32.89 AS.reconfigure ASfast @00:01:32.95 AS.seek 0ml @00:02:00.29 AFV.select :carrierSolution @00:02:00.40 delay 2 seconds @00:02:02.44 AS.reconfigure ASconfig @00:02:02.48 AS.seek 1ml @00:03:00.23 delay 1 second @00:03:01.26 AFV.select :holdingCoil @00:03:01.36 delay 1 second @00:03:02.40 AS.reconfigure ASfast @00:03:02.45 AS.seek 0ml @00:03:29.78 AFV.select :carrierSolution @00:03:29.89 delay 2 seconds @00:03:31.93 AS.reconfigure ASconfig @00:03:31.98 AS.seek 1ml @00:04:29.76 delay 1 second @00:04:30.78 AFV.select :holdingCoil @00:04:30.88 delay 1 second @00:04:31.92 AS.reconfigure ASfast @00:04:31.97 AS.seek 0ml @00:04:59.34 ARV.dial between carrier and air1 @00:04:59.87 ATV.dial :air3 @00:05:01.14 AS.reconfigure ASslow1 @00:05:01.20 AS.seek 0.01ml @00:05:03.59 delay 2 seconds @00:05:05.62 ATV.dial :bleach @00:05:06.25 AS.seek 0.81ml @00:07:47.56 delay 3 seconds @00:07:50.59 ATV.dial :air3 @00:07:51.12 delay 3 seconds @00:07:54.21 AS.seek 0.835ml @00:07:59.54 delay 3 seconds @00:08:02.57 ATV.dial :bleach @00:08:03.20 AS.seek 0.82ml @00:08:06.51 delay 3 seconds @00:08:10.06 ATV.dial PCR @00:08:10.49 AS.reconfigure ASconfig @00:08:10.54 AS.seek :empty @00:08:58.00 PCR.program :bleachProfile @00:08:58.06 PCR program should complete in < 2:45 @00:08:58.08 PCR.run 10:00 @00:11:50.79 PCR.off @00:11:50.82 AFV.select :carrierSolution @00:11:50.92 delay 2 seconds @00:11:52.96 AS.seek 1ml @00:12:50.78 delay 1 second @00:12:51.80 AFV.select :holdingCoil @00:12:51.90 delay 1 second @00:12:52.94 AS.reconfigure ASfast @00:12:52.98 AS.seek 0ml @00:13:20.34 delay 3 seconds @00:13:23.37 AFV.select :carrierSolution @00:13:23.47 delay 2 seconds @00:13:25.51 AS.reconfigure ASconfig @00:13:25.56 AS.seek 1ml @00:14:23.32 delay 1 second @00:14:24.34 AFV.select :holdingCoil @00:14:24.44 delay 1 second @00:14:25.48 AS.reconfigure ASfast @00:14:25.53 AS.seek 0ml @00:14:52.89 delay 3 seconds @00:14:55.92 AFV.select :carrierSolution @00:14:56.02 delay 2 seconds @00:14:58.06 AS.reconfigure ASconfig @00:14:58.11 AS.seek 1ml @00:15:55.89 delay 1 second @00:15:56.91 AFV.select :holdingCoil @00:15:57.01 delay 1 second @00:15:58.05 AS.reconfigure ASfast @00:15:58.10 AS.seek 0ml @00:16:25.45 delay 3 seconds @00:16:28.48 AFV.select :carrierSolution @00:16:28.58 delay 2 seconds @00:16:30.63 AS.reconfigure ASconfig @00:16:30.68 AS.seek 1ml @00:17:28.43 delay 1 second @00:17:29.45 AFV.select :holdingCoil @00:17:29.55 delay 1 second @00:17:30.59 AS.reconfigure ASfast @00:17:30.64 AS.seek 0ml @00:17:57.99 delay 3 seconds @00:18:01.02 AFV.select :carrierSolution @00:18:01.12 delay 2 seconds @00:18:03.16 AS.reconfigure ASconfig @00:18:03.21 AS.seek 1ml @00:19:00.99 delay 1 second @00:19:02.01 AFV.select :holdingCoil @00:19:02.11 delay 1 second @00:19:03.15 AS.reconfigure ASnorm @00:19:03.19 AS.seek 0ml @00:19:53.93 Setting up PCR for Rp -- writing /var/log/esp/Rp18sep0700h.pcr @00:19:53.95 Building PCR Slug from ASV with MM2 mix, PP3 primer @00:19:53.96 Priming with carrier @00:19:53.97 ATV.dial ARV @00:19:54.57 ARV.dial :carrier @00:19:54.95 AS.seek 0.5ml @00:20:20.48 AFV.select :carrierSolution @00:20:20.59 delay 2 seconds @00:20:22.63 AS.reconfigure ASconfig @00:20:22.68 AS.seek 1ml @00:20:51.68 delay 1 second @00:20:52.71 AFV.select :holdingCoil @00:20:52.81 delay 1 second @00:20:53.84 ATV.dial :waste @00:20:55.50 AS.reconfigure ASnorm @00:20:55.55 AS.seek :empty @00:21:46.29 AFV.select :carrierSolution @00:21:46.40 delay 2 seconds @00:21:48.44 AS.reconfigure ASconfig @00:21:48.48 AS.seek 0.5ml @00:22:17.54 delay 1 second @00:22:18.56 AFV.select :holdingCoil @00:22:18.67 Building Slug @00:22:18.68 ATV.dial :ARV @00:22:20.30 delay 2 seconds @00:22:22.32 ARV.dial :air1 @00:22:22.71 delay 2 seconds @00:22:24.79 AS.reconfigure ASslow2 @00:22:24.85 AS.seek 0.506ml @00:22:31.12 delay 2 seconds @00:22:33.15 ARV.dial :MM2 @00:22:34.67 delay 2 seconds @00:22:36.71 Adding MM2 @00:22:36.78 AS.seek 0.524ml @00:22:54.99 delay 25 seconds @00:23:20.02 ARV.dial :PP3 @00:23:20.43 Adding PP3 @00:23:20.44 delay 2 seconds @00:23:22.52 AS.seek 0.53ml @00:23:28.96 delay 10 seconds @00:23:38.99 ARV.dial :air2 @00:23:39.40 adding ARV air @00:23:39.41 delay 2 seconds @00:23:41.49 AS.seek 0.536ml @00:23:47.93 delay 5 seconds @00:23:53.51 ARV.dial :carrier @00:23:54.84 delay 7 seconds @00:24:01.94 AS.seek 0.594ml @00:25:00.67 delay 5 seconds @00:25:05.70 ATV.dial :ASV @00:25:06.09 delay 2 seconds @00:25:08.19 AS.seek 0.6ml @00:25:14.45 delay 4 seconds @00:25:18.48 ATV.dial ARV @00:25:19.02 delay 2 seconds @00:25:21.04 ARV.dial :carrier @00:25:21.06 delay 2 seconds @00:25:23.15 AS.seek 0.65ml @00:26:13.81 delay 4 seconds @00:26:17.84 ATV.dial PCR @00:26:18.45 ASV.dial :waste1 @00:26:18.47 ARV.dial :air1 @00:26:18.89 Positioning Slug by 0.324ml @00:26:18.98 AS.seek 0.326ml @00:31:45.04 delay 5 seconds @00:31:50.06 ATV.dial ARV @00:31:50.82 ASV.dial :PCRlock @00:31:51.24 delay 5 seconds @00:31:56.26 Running Rp Profile60C300 thermocycling @00:31:56.28 PCR.program Profile60C300 @00:31:56.41 Profile60C300 should complete in < 1:45:21 @00:31:56.43 PCR.run 2:16:41.25 @01:57:03.28 PCR.off @01:57:04.88 PCR for Rp completed -- closed /var/log/esp/Rp18sep0700h.pcr @01:57:04.96 Cleaning PCR @01:57:04.97 AFV.select :carrierSolution @01:57:05.07 delay 2 seconds @01:57:07.11 AS.reconfigure ASconfig @01:57:07.15 AS.seek 0.5ml @01:57:17.47 delay 1 second @01:57:18.49 AFV.select :holdingCoil @01:57:18.59 ASV.dial :waste1 @01:57:18.99 ATV.dial :air3 @01:57:20.28 AS.reconfigure ASslow1 @01:57:20.34 AS.seek 0.51ml @01:57:22.64 delay 2 seconds @01:57:24.66 ATV.dial :bleach @01:57:25.29 AS.seek 0.81ml @01:58:25.89 delay 3 seconds @01:58:28.92 ATV.dial :air3 @01:58:29.46 delay 3 seconds @01:58:32.55 AS.seek 0.835ml @01:58:37.77 delay 3 seconds @01:58:40.80 ATV.dial :bleach @01:58:41.43 AS.seek 0.82ml @01:58:44.72 delay 3 seconds @01:58:47.75 ARV.dial :waste @01:58:48.36 delay 3 seconds @01:58:51.39 ATV.dial :ARV @01:58:52.28 AS.reconfigure ASconfig @01:58:52.34 AS.seek 0.47ml @01:59:12.75 AFV.select :carrierSolution @01:59:12.86 delay 2 seconds @01:59:14.90 AS.seek 1ml @01:59:45.72 delay 1 second @01:59:46.74 AFV.select :holdingCoil @01:59:46.84 delay 1 second @01:59:47.88 AS.reconfigure ASfast @01:59:47.92 AS.seek 0ml @02:00:15.28 AFV.select :carrierSolution @02:00:15.39 delay 2 seconds @02:00:17.43 AS.reconfigure ASconfig @02:00:17.48 AS.seek 1ml @02:01:15.24 delay 1 second @02:01:16.26 AFV.select :holdingCoil @02:01:16.36 delay 1 second @02:01:17.40 AS.reconfigure ASfast @02:01:17.44 AS.seek 0ml @02:01:44.80 AFV.select :carrierSolution @02:01:44.91 delay 2 seconds @02:01:46.95 AS.reconfigure ASconfig @02:01:46.99 AS.seek 1ml @02:02:44.78 delay 1 second @02:02:45.80 AFV.select :holdingCoil @02:02:45.90 delay 1 second @02:02:46.94 AS.reconfigure ASfast @02:02:46.98 AS.seek 0ml @02:03:14.32 ARV.dial between carrier and air1 @02:03:14.83 ATV.dial :air3 @02:03:16.12 AS.reconfigure ASslow1 @02:03:16.18 AS.seek 0.01ml @02:03:18.53 delay 2 seconds @02:03:20.55 ATV.dial :bleach @02:03:21.17 AS.seek 0.81ml @02:06:02.33 delay 3 seconds @02:06:05.36 ATV.dial :air3 @02:06:05.89 delay 3 seconds @02:06:08.98 AS.seek 0.835ml @02:06:14.82 delay 3 seconds @02:06:17.84 ATV.dial :bleach @02:06:18.47 AS.seek 0.82ml @02:06:21.77 delay 3 seconds @02:06:24.80 ATV.dial PCR @02:06:25.20 AS.reconfigure ASconfig @02:06:25.25 AS.seek :empty @02:07:12.72 PCR.program :bleachProfile @02:07:12.78 PCR program should complete in < 2:45 @02:07:12.79 PCR.run 10:00 @02:10:05.52 PCR.off @02:10:05.55 AFV.select :carrierSolution @02:10:05.66 delay 2 seconds @02:10:07.70 AS.seek 1ml @02:11:05.55 delay 1 second @02:11:06.57 AFV.select :holdingCoil @02:11:06.67 delay 1 second @02:11:07.71 AS.reconfigure ASfast @02:11:07.75 AS.seek 0ml @02:11:35.13 delay 3 seconds @02:11:38.16 AFV.select :carrierSolution @02:11:38.26 delay 2 seconds @02:11:40.30 AS.reconfigure ASconfig @02:11:40.35 AS.seek 1ml @02:12:38.10 delay 1 second @02:12:39.13 AFV.select :holdingCoil @02:12:39.23 delay 1 second @02:12:40.27 AS.reconfigure ASfast @02:12:40.31 AS.seek 0ml @02:13:07.67 delay 3 seconds @02:13:10.70 AFV.select :carrierSolution @02:13:10.80 delay 2 seconds @02:13:12.84 AS.reconfigure ASconfig @02:13:12.90 AS.seek 1ml @02:14:10.64 delay 1 second @02:14:11.66 AFV.select :holdingCoil @02:14:11.76 delay 1 second @02:14:12.80 AS.reconfigure ASfast @02:14:12.84 AS.seek 0ml @02:14:40.17 delay 3 seconds @02:14:43.20 AFV.select :carrierSolution @02:14:43.30 delay 2 seconds @02:14:45.34 AS.reconfigure ASconfig @02:14:45.39 AS.seek 1ml @02:15:43.17 delay 1 second @02:15:44.19 AFV.select :holdingCoil @02:15:44.29 delay 1 second @02:15:45.33 AS.reconfigure ASfast @02:15:45.38 AS.seek 0ml @02:16:12.72 delay 3 seconds @02:16:15.75 AFV.select :carrierSolution @02:16:15.85 delay 2 seconds @02:16:17.89 AS.reconfigure ASconfig @02:16:17.94 AS.seek 1ml @02:17:15.72 delay 1 second @02:17:16.74 AFV.select :holdingCoil @02:17:16.84 delay 1 second @02:17:17.87 AS.reconfigure ASnorm @02:17:17.91 AS.seek 0ml @02:18:08.64 Setting up PCR for Ss -- writing /var/log/esp/Ss18sep0702h.pcr @02:18:08.65 Building PCR Slug from ASV with MM1 mix, PP2 primer @02:18:08.67 Priming with carrier @02:18:08.68 ATV.dial ARV @02:18:09.30 ARV.dial :carrier @02:18:09.68 AS.seek 0.5ml @02:18:35.22 AFV.select :carrierSolution @02:18:35.33 delay 2 seconds @02:18:37.37 AS.reconfigure ASconfig @02:18:37.42 AS.seek 1ml @02:19:06.44 delay 1 second @02:19:07.46 AFV.select :holdingCoil @02:19:07.56 delay 1 second @02:19:08.59 ATV.dial :waste @02:19:10.26 AS.reconfigure ASnorm @02:19:10.31 AS.seek :empty @02:20:01.05 AFV.select :carrierSolution @02:20:01.19 delay 2 seconds @02:20:03.23 AS.reconfigure ASconfig @02:20:03.27 AS.seek 0.5ml @02:20:32.39 delay 1 second @02:20:33.41 AFV.select :holdingCoil @02:20:33.52 Building Slug @02:20:33.53 ATV.dial :ARV @02:20:35.15 delay 2 seconds @02:20:37.17 ARV.dial :air1 @02:20:37.66 delay 2 seconds @02:20:39.76 AS.reconfigure ASslow2 @02:20:39.81 AS.seek 0.506ml @02:20:46.21 delay 2 seconds @02:20:48.24 ARV.dial :MM1 @02:20:48.86 delay 2 seconds @02:20:50.90 Adding MM1 @02:20:50.97 AS.seek 0.524ml @02:21:09.31 delay 25 seconds @02:21:34.34 ARV.dial :PP2 @02:21:34.75 Adding PP2 @02:21:34.76 delay 2 seconds @02:21:36.84 AS.seek 0.53ml @02:21:43.08 delay 10 seconds @02:21:53.11 ARV.dial :air2 @02:21:54.07 adding ARV air @02:21:54.08 delay 2 seconds @02:21:56.17 AS.seek 0.536ml @02:22:02.58 delay 5 seconds @02:22:07.61 ARV.dial :carrier @02:22:08.91 delay 7 seconds @02:22:16.01 AS.seek 0.594ml @02:23:14.58 delay 5 seconds @02:23:19.61 ATV.dial :ASV @02:23:20.00 delay 2 seconds @02:23:22.10 AS.seek 0.6ml @02:23:28.25 delay 4 seconds @02:23:32.28 ATV.dial ARV @02:23:32.81 delay 2 seconds @02:23:34.84 ARV.dial :carrier @02:23:34.86 delay 2 seconds @02:23:36.94 AS.seek 0.65ml @02:24:27.46 delay 4 seconds @02:24:31.49 ATV.dial PCR @02:24:32.10 ASV.dial :waste1 @02:24:32.12 ARV.dial :air1 @02:24:32.54 Positioning Slug by 0.324ml @02:24:32.63 AS.seek 0.326ml @02:29:58.70 delay 5 seconds @02:30:03.73 ATV.dial ARV @02:30:04.50 ASV.dial :PCRlock @02:30:04.92 delay 5 seconds @02:30:09.94 Running Ss Profile60C350 thermocycling @02:30:09.96 PCR.program Profile60C350 @02:30:10.09 Profile60C350 should complete in < 1:48:05 @02:30:10.11 PCR.run 2:20:06.25 @03:55:27.35 PCR.off @03:55:29.44 PCR for Ss completed -- closed /var/log/esp/Ss18sep0702h.pcr @03:55:29.52 Cleaning PCR @03:55:29.53 AFV.select :carrierSolution @03:55:29.64 delay 2 seconds @03:55:31.68 AS.reconfigure ASconfig @03:55:31.73 AS.seek 0.5ml @03:55:42.03 delay 1 second @03:55:43.05 AFV.select :holdingCoil @03:55:43.15 ASV.dial :waste1 @03:55:43.54 ATV.dial :air3 @03:55:44.83 AS.reconfigure ASslow1 @03:55:44.89 AS.seek 0.51ml @03:55:47.18 delay 2 seconds @03:55:49.20 ATV.dial :bleach @03:55:49.83 AS.seek 0.81ml @03:56:50.51 delay 3 seconds @03:56:53.54 ATV.dial :air3 @03:56:54.07 delay 3 seconds @03:56:57.16 AS.seek 0.835ml @03:57:02.51 delay 3 seconds @03:57:05.54 ATV.dial :bleach @03:57:06.17 AS.seek 0.82ml @03:57:09.46 delay 3 seconds @03:57:12.49 ARV.dial :waste @03:57:13.11 delay 3 seconds @03:57:16.14 ATV.dial :ARV @03:57:17.14 AS.reconfigure ASconfig @03:57:17.19 AS.seek 0.47ml @03:57:37.59 AFV.select :carrierSolution @03:57:37.70 delay 2 seconds @03:57:39.74 AS.seek 1ml @03:58:10.51 delay 1 second @03:58:11.53 AFV.select :holdingCoil @03:58:11.63 delay 1 second @03:58:12.67 AS.reconfigure ASfast @03:58:12.72 AS.seek 0ml @03:58:40.06 AFV.select :carrierSolution @03:58:40.17 delay 2 seconds @03:58:42.21 AS.reconfigure ASconfig @03:58:42.26 AS.seek 1ml @03:59:40.01 delay 1 second @03:59:41.03 AFV.select :holdingCoil @03:59:41.13 delay 1 second @03:59:42.17 AS.reconfigure ASfast @03:59:42.21 AS.seek 0ml @04:00:09.56 AFV.select :carrierSolution @04:00:09.67 delay 2 seconds @04:00:11.71 AS.reconfigure ASconfig @04:00:11.76 AS.seek 1ml @04:01:09.59 delay 1 second @04:01:10.61 AFV.select :holdingCoil @04:01:10.71 delay 1 second @04:01:11.75 AS.reconfigure ASfast @04:01:11.79 AS.seek 0ml @04:01:39.17 ARV.dial between carrier and air1 @04:01:39.69 ATV.dial :air3 @04:01:41.52 AS.reconfigure ASslow1 @04:01:41.58 AS.seek 0.01ml @04:01:43.93 delay 2 seconds @04:01:45.96 ATV.dial :bleach @04:01:46.59 AS.seek 0.81ml @04:04:27.76 delay 3 seconds @04:04:30.79 ATV.dial :air3 @04:04:31.32 delay 3 seconds @04:04:34.41 AS.seek 0.835ml @04:04:39.68 delay 3 seconds @04:04:42.71 ATV.dial :bleach @04:04:43.34 AS.seek 0.82ml @04:04:46.64 delay 3 seconds @04:04:49.67 ATV.dial PCR @04:04:50.10 AS.reconfigure ASconfig @04:04:50.15 AS.seek :empty @04:05:37.61 PCR.program :bleachProfile @04:05:37.66 PCR program should complete in < 2:45 @04:05:37.68 PCR.run 10:00 @04:08:30.39 PCR.off @04:08:30.42 AFV.select :carrierSolution @04:08:30.53 delay 2 seconds @04:08:32.57 AS.seek 1ml @04:09:30.40 delay 1 second @04:09:31.42 AFV.select :holdingCoil @04:09:31.52 delay 1 second @04:09:32.56 AS.reconfigure ASfast @04:09:32.61 AS.seek 0ml @04:09:59.96 delay 3 seconds @04:10:02.99 AFV.select :carrierSolution @04:10:03.09 delay 2 seconds @04:10:05.13 AS.reconfigure ASconfig @04:10:05.18 AS.seek 1ml @04:11:02.96 delay 1 second @04:11:03.98 AFV.select :holdingCoil @04:11:04.08 delay 1 second @04:11:05.12 AS.reconfigure ASfast @04:11:05.17 AS.seek 0ml @04:11:32.51 delay 3 seconds @04:11:35.53 AFV.select :carrierSolution @04:11:35.63 delay 2 seconds @04:11:37.67 AS.reconfigure ASconfig @04:11:37.71 AS.seek 1ml @04:12:35.53 delay 1 second @04:12:36.55 AFV.select :holdingCoil @04:12:36.65 delay 1 second @04:12:37.69 AS.reconfigure ASfast @04:12:37.74 AS.seek 0ml @04:13:05.10 delay 3 seconds @04:13:08.13 AFV.select :carrierSolution @04:13:08.23 delay 2 seconds @04:13:10.27 AS.reconfigure ASconfig @04:13:10.33 AS.seek 1ml @04:14:08.09 delay 1 second @04:14:09.11 AFV.select :holdingCoil @04:14:09.21 delay 1 second @04:14:10.25 AS.reconfigure ASfast @04:14:10.29 AS.seek 0ml @04:14:37.65 delay 3 seconds @04:14:40.68 AFV.select :carrierSolution @04:14:40.78 delay 2 seconds @04:14:42.82 AS.reconfigure ASconfig @04:14:42.87 AS.seek 1ml @04:15:40.65 delay 1 second @04:15:41.67 AFV.select :holdingCoil @04:15:41.77 delay 1 second @04:15:42.81 AS.reconfigure ASnorm @04:15:42.85 AS.seek 0ml @04:16:33.58 Setting up PCR for Tt2 -- writing /var/log/esp/Tt218sep0704h.pcr @04:16:33.60 Building PCR Slug from ASV with MM3 mix, PP6 primer @04:16:33.62 Priming with carrier @04:16:33.63 ATV.dial ARV @04:16:34.23 ARV.dial :carrier @04:16:34.61 AS.seek 0.5ml @04:17:00.21 AFV.select :carrierSolution @04:17:00.32 delay 2 seconds @04:17:02.36 AS.reconfigure ASconfig @04:17:02.40 AS.seek 1ml @04:17:31.42 delay 1 second @04:17:32.45 AFV.select :holdingCoil @04:17:32.55 delay 1 second @04:17:33.58 ATV.dial :waste @04:17:35.24 AS.reconfigure ASnorm @04:17:35.29 AS.seek :empty @04:18:26.03 AFV.select :carrierSolution @04:18:26.14 delay 2 seconds @04:18:28.18 AS.reconfigure ASconfig @04:18:28.22 AS.seek 0.5ml @04:18:57.31 delay 1 second @04:18:58.33 AFV.select :holdingCoil @04:18:58.44 Building Slug @04:18:58.45 ATV.dial :ARV @04:19:00.07 delay 2 seconds @04:19:02.09 ARV.dial :air1 @04:19:02.50 delay 2 seconds @04:19:04.58 AS.reconfigure ASslow2 @04:19:04.64 AS.seek 0.506ml @04:19:10.85 delay 2 seconds @04:19:12.88 ARV.dial :MM3 @04:19:14.53 delay 2 seconds @04:19:16.55 Adding MM3 @04:19:16.64 AS.seek 0.524ml @04:19:34.89 delay 25 seconds @04:19:59.92 ARV.dial :PP6 @04:20:00.33 Adding PP6 @04:20:00.34 delay 2 seconds @04:20:02.43 AS.seek 0.53ml @04:20:08.60 delay 10 seconds @04:20:18.63 ARV.dial :air1 @04:20:19.47 adding ARV air @04:20:19.48 delay 2 seconds @04:20:21.57 AS.seek 0.536ml @04:20:27.85 delay 5 seconds @04:20:32.88 ARV.dial :carrier @04:20:33.29 delay 7 seconds @04:20:40.39 AS.seek 0.594ml @04:21:39.21 delay 5 seconds @04:21:44.24 ATV.dial :ASV @04:21:44.65 delay 2 seconds @04:21:46.75 AS.seek 0.6ml @04:21:53.12 delay 4 seconds @04:21:57.15 ATV.dial ARV @04:21:57.68 delay 2 seconds @04:21:59.71 ARV.dial :carrier @04:21:59.73 delay 2 seconds @04:22:01.82 AS.seek 0.65ml @04:22:52.28 delay 4 seconds @04:22:56.31 ATV.dial PCR @04:22:57.03 ASV.dial :waste1 @04:22:57.05 ARV.dial :air1 @04:22:57.48 Positioning Slug by 0.324ml @04:22:57.58 AS.seek 0.326ml @04:28:23.85 delay 5 seconds @04:28:28.88 ATV.dial ARV @04:28:29.74 ASV.dial :PCRlock @04:28:30.16 delay 5 seconds @04:28:35.18 Running Tt2 Profile60C300 thermocycling @04:28:35.20 PCR.program Profile60C300 @04:28:35.33 Profile60C300 should complete in < 1:45:21 @04:28:35.35 PCR.run 2:16:41.25 @05:53:26.57 PCR.off @05:53:28.16 PCR for Tt2 completed -- closed /var/log/esp/Tt218sep0704h.pcr @05:53:28.23 Cleaning PCR @05:53:28.24 AFV.select :carrierSolution @05:53:28.35 delay 2 seconds @05:53:30.39 AS.reconfigure ASconfig @05:53:30.43 AS.seek 0.5ml @05:53:40.72 delay 1 second @05:53:41.74 AFV.select :holdingCoil @05:53:41.84 ASV.dial :waste1 @05:53:42.32 ATV.dial :air3 @05:53:43.61 AS.reconfigure ASslow1 @05:53:43.67 AS.seek 0.51ml @05:53:45.98 delay 2 seconds @05:53:48.00 ATV.dial :bleach @05:53:48.63 AS.seek 0.81ml @05:54:49.21 delay 3 seconds @05:54:52.24 ATV.dial :air3 @05:54:52.77 delay 3 seconds @05:54:55.86 AS.seek 0.835ml @05:55:01.08 delay 3 seconds @05:55:04.11 ATV.dial :bleach @05:55:04.74 AS.seek 0.82ml @05:55:08.04 delay 3 seconds @05:55:11.07 ARV.dial :waste @05:55:11.69 delay 3 seconds @05:55:14.72 ATV.dial :ARV @05:55:15.72 AS.reconfigure ASconfig @05:55:15.78 AS.seek 0.47ml @05:55:36.18 AFV.select :carrierSolution @05:55:36.29 delay 2 seconds @05:55:38.33 AS.seek 1ml @05:56:09.10 delay 1 second @05:56:10.12 AFV.select :holdingCoil @05:56:10.22 delay 1 second @05:56:11.26 AS.reconfigure ASfast @05:56:11.31 AS.seek 0ml @05:56:38.66 AFV.select :carrierSolution @05:56:38.77 delay 2 seconds @05:56:40.81 AS.reconfigure ASconfig @05:56:40.86 AS.seek 1ml @05:57:38.63 delay 1 second @05:57:39.65 AFV.select :holdingCoil @05:57:39.75 delay 1 second @05:57:40.79 AS.reconfigure ASfast @05:57:40.84 AS.seek 0ml @05:58:08.18 AFV.select :carrierSolution @05:58:08.29 delay 2 seconds @05:58:10.33 AS.reconfigure ASconfig @05:58:10.37 AS.seek 1ml @05:59:08.16 delay 1 second @05:59:09.18 AFV.select :holdingCoil @05:59:09.28 delay 1 second @05:59:10.32 AS.reconfigure ASfast @05:59:10.37 AS.seek 0ml @05:59:37.72 ARV.dial between carrier and air1 @05:59:38.24 ATV.dial :air3 @05:59:40.09 AS.reconfigure ASslow1 @05:59:40.14 AS.seek 0.01ml @05:59:42.47 delay 2 seconds @05:59:44.49 ATV.dial :bleach @05:59:45.13 AS.seek 0.81ml @06:02:26.32 delay 3 seconds @06:02:29.35 ATV.dial :air3 @06:02:29.90 delay 3 seconds @06:02:32.98 AS.seek 0.835ml @06:02:38.28 delay 3 seconds @06:02:41.31 ATV.dial :bleach @06:02:41.94 AS.seek 0.82ml @06:02:45.25 delay 3 seconds @06:02:48.28 ATV.dial PCR @06:02:48.70 AS.reconfigure ASconfig @06:02:48.75 AS.seek :empty @06:03:36.22 PCR.program :bleachProfile @06:03:36.28 PCR program should complete in < 2:45 @06:03:36.29 PCR.run 10:00 @06:06:32.05 PCR.off @06:06:32.08 AFV.select :carrierSolution @06:06:32.19 delay 2 seconds @06:06:34.23 AS.seek 1ml @06:07:32.07 delay 1 second @06:07:33.09 AFV.select :holdingCoil @06:07:33.19 delay 1 second @06:07:34.23 AS.reconfigure ASfast @06:07:34.28 AS.seek 0ml @06:08:01.63 delay 3 seconds @06:08:04.66 AFV.select :carrierSolution @06:08:04.76 delay 2 seconds @06:08:06.80 AS.reconfigure ASconfig @06:08:06.86 AS.seek 1ml @06:09:04.64 delay 1 second @06:09:05.67 AFV.select :holdingCoil @06:09:05.77 delay 1 second @06:09:06.81 AS.reconfigure ASfast @06:09:06.85 AS.seek 0ml @06:09:34.21 delay 3 seconds @06:09:37.24 AFV.select :carrierSolution @06:09:37.34 delay 2 seconds @06:09:39.38 AS.reconfigure ASconfig @06:09:39.43 AS.seek 1ml @06:10:37.21 delay 1 second @06:10:38.23 AFV.select :holdingCoil @06:10:38.33 delay 1 second @06:10:39.37 AS.reconfigure ASfast @06:10:39.42 AS.seek 0ml @06:11:06.77 delay 3 seconds @06:11:09.80 AFV.select :carrierSolution @06:11:09.90 delay 2 seconds @06:11:11.94 AS.reconfigure ASconfig @06:11:11.98 AS.seek 1ml @06:12:09.77 delay 1 second @06:12:10.79 AFV.select :holdingCoil @06:12:10.89 delay 1 second @06:12:11.93 AS.reconfigure ASfast @06:12:11.98 AS.seek 0ml @06:12:39.33 delay 3 seconds @06:12:42.36 AFV.select :carrierSolution @06:12:42.46 delay 2 seconds @06:12:44.50 AS.reconfigure ASconfig @06:12:44.55 AS.seek 1ml @06:13:42.35 delay 1 second @06:13:43.37 AFV.select :holdingCoil @06:13:43.47 delay 1 second @06:13:44.51 AS.reconfigure ASnorm @06:13:44.55 AS.seek 0ml @06:14:35.30 Setting up PCR for Bb -- writing /var/log/esp/Bb18sep0706h.pcr @06:14:35.32 Building PCR Slug from ASV with MM3 mix, PP5 primer @06:14:35.33 Priming with carrier @06:14:35.34 ATV.dial ARV @06:14:35.94 ARV.dial :carrier @06:14:36.32 AS.seek 0.5ml @06:15:01.88 AFV.select :carrierSolution @06:15:01.99 delay 2 seconds @06:15:04.03 AS.reconfigure ASconfig @06:15:04.08 AS.seek 1ml @06:15:33.10 delay 1 second @06:15:34.13 AFV.select :holdingCoil @06:15:34.23 delay 1 second @06:15:35.26 ATV.dial :waste @06:15:36.92 AS.reconfigure ASnorm @06:15:36.97 AS.seek :empty @06:16:27.69 AFV.select :carrierSolution @06:16:27.80 delay 2 seconds @06:16:29.84 AS.reconfigure ASconfig @06:16:29.89 AS.seek 0.5ml @06:16:58.97 delay 1 second @06:16:59.99 AFV.select :holdingCoil @06:17:00.10 Building Slug @06:17:00.11 ATV.dial :ARV @06:17:01.73 delay 2 seconds @06:17:03.75 ARV.dial :air1 @06:17:04.14 delay 2 seconds @06:17:06.23 AS.reconfigure ASslow2 @06:17:06.28 AS.seek 0.506ml @06:17:12.80 delay 2 seconds @06:17:14.83 ARV.dial :MM3 @06:17:15.88 delay 2 seconds @06:17:18.48 Adding MM3 @06:17:18.57 AS.seek 0.524ml @06:17:36.85 delay 25 seconds @06:18:01.87 ARV.dial :PP5 @06:18:02.28 Adding PP5 @06:18:02.29 delay 2 seconds @06:18:04.38 AS.seek 0.53ml @06:18:10.77 delay 10 seconds @06:18:20.81 ARV.dial :air3 @06:18:21.20 adding ARV air @06:18:21.21 delay 2 seconds @06:18:23.29 AS.seek 0.536ml @06:18:29.72 delay 5 seconds @06:18:34.75 ARV.dial :carrier @06:18:36.03 delay 7 seconds @06:18:43.12 AS.seek 0.594ml @06:19:41.86 delay 5 seconds @06:19:46.89 ATV.dial :ASV @06:19:47.40 delay 2 seconds @06:19:49.48 AS.seek 0.6ml @06:19:55.74 delay 4 seconds @06:19:59.77 ATV.dial ARV @06:20:00.41 delay 2 seconds @06:20:02.44 ARV.dial :carrier @06:20:02.46 delay 2 seconds @06:20:04.55 AS.seek 0.65ml @06:20:55.27 delay 4 seconds @06:20:59.30 ATV.dial PCR @06:20:59.91 ASV.dial :waste1 @06:20:59.93 ARV.dial :air1 @06:21:00.35 Positioning Slug by 0.324ml @06:21:00.44 AS.seek 0.326ml @06:26:26.41 delay 5 seconds @06:26:31.44 ATV.dial ARV @06:26:32.21 ASV.dial :PCRlock @06:26:32.60 delay 5 seconds @06:26:37.63 Running Bb Profile60C300 thermocycling @06:26:37.65 PCR.program Profile60C300 @06:26:37.78 Profile60C300 should complete in < 1:45:21 @06:26:37.80 PCR.run 2:16:41.25 @07:51:34.43 PCR.off @07:51:36.03 PCR for Bb completed -- closed /var/log/esp/Bb18sep0706h.pcr @07:51:36.10 Cleaning PCR @07:51:36.11 AFV.select :carrierSolution @07:51:36.22 delay 2 seconds @07:51:38.26 AS.reconfigure ASconfig @07:51:38.30 AS.seek 0.5ml @07:51:48.61 delay 1 second @07:51:49.63 AFV.select :holdingCoil @07:51:49.73 ASV.dial :waste1 @07:51:50.12 ATV.dial :air3 @07:51:51.41 AS.reconfigure ASslow1 @07:51:51.47 AS.seek 0.51ml @07:51:53.75 delay 2 seconds @07:51:55.78 ATV.dial :bleach @07:51:56.41 AS.seek 0.81ml @07:52:57.11 delay 3 seconds @07:53:00.14 ATV.dial :air3 @07:53:00.68 delay 3 seconds @07:53:03.77 AS.seek 0.835ml @07:53:09.10 delay 3 seconds @07:53:12.13 ATV.dial :bleach @07:53:12.75 AS.seek 0.82ml @07:53:16.06 delay 3 seconds @07:53:19.09 ARV.dial :waste @07:53:19.70 delay 3 seconds @07:53:22.73 ATV.dial :ARV @07:53:23.72 AS.reconfigure ASconfig @07:53:23.77 AS.seek 0.47ml @07:53:44.18 AFV.select :carrierSolution @07:53:44.29 delay 2 seconds @07:53:46.33 AS.seek 1ml @07:54:17.12 delay 1 second @07:54:18.14 AFV.select :holdingCoil @07:54:18.24 delay 1 second @07:54:19.28 AS.reconfigure ASfast @07:54:19.32 AS.seek 0ml @07:54:46.67 AFV.select :carrierSolution @07:54:46.78 delay 2 seconds @07:54:48.82 AS.reconfigure ASconfig @07:54:48.86 AS.seek 1ml @07:55:46.62 delay 1 second @07:55:47.64 AFV.select :holdingCoil @07:55:47.74 delay 1 second @07:55:48.78 AS.reconfigure ASfast @07:55:48.82 AS.seek 0ml @07:56:16.17 AFV.select :carrierSolution @07:56:16.28 delay 2 seconds @07:56:18.32 AS.reconfigure ASconfig @07:56:18.37 AS.seek 1ml @07:57:16.11 delay 1 second @07:57:17.13 AFV.select :holdingCoil @07:57:17.23 delay 1 second @07:57:18.27 AS.reconfigure ASfast @07:57:18.31 AS.seek 0ml @07:57:45.67 ARV.dial between carrier and air1 @07:57:46.19 ATV.dial :air3 @07:57:47.47 AS.reconfigure ASslow1 @07:57:48.08 AS.seek 0.01ml @07:57:50.42 delay 2 seconds @07:57:52.44 ATV.dial :bleach @07:57:53.08 AS.seek 0.81ml @08:00:34.29 delay 3 seconds @08:00:37.31 ATV.dial :air3 @08:00:37.86 delay 3 seconds @08:00:40.95 AS.seek 0.835ml @08:00:46.26 delay 3 seconds @08:00:49.29 ATV.dial :bleach @08:00:49.92 AS.seek 0.82ml @08:00:53.17 delay 3 seconds @08:00:56.19 ATV.dial PCR @08:00:56.62 AS.reconfigure ASconfig @08:00:56.67 AS.seek :empty @08:01:44.11 PCR.program :bleachProfile @08:01:44.17 PCR program should complete in < 2:45 @08:01:44.18 PCR.run 10:00 @08:04:36.91 PCR.off @08:04:36.94 AFV.select :carrierSolution @08:04:37.04 delay 2 seconds @08:04:39.08 AS.seek 1ml @08:05:36.90 delay 1 second @08:05:37.92 AFV.select :holdingCoil @08:05:38.02 delay 1 second @08:05:39.06 AS.reconfigure ASfast @08:05:39.11 AS.seek 0ml @08:06:06.45 delay 3 seconds @08:06:09.48 AFV.select :carrierSolution @08:06:09.58 delay 2 seconds @08:06:11.63 AS.reconfigure ASconfig @08:06:11.68 AS.seek 1ml @08:07:09.42 delay 1 second @08:07:10.44 AFV.select :holdingCoil @08:07:10.54 delay 1 second @08:07:11.58 AS.reconfigure ASfast @08:07:11.62 AS.seek 0ml @08:07:38.98 delay 3 seconds @08:07:42.00 AFV.select :carrierSolution @08:07:42.10 delay 2 seconds @08:07:44.14 AS.reconfigure ASconfig @08:07:44.19 AS.seek 1ml @08:08:41.96 delay 1 second @08:08:42.98 AFV.select :holdingCoil @08:08:43.08 delay 1 second @08:08:44.12 AS.reconfigure ASfast @08:08:44.16 AS.seek 0ml @08:09:11.51 delay 3 seconds @08:09:14.54 AFV.select :carrierSolution @08:09:14.64 delay 2 seconds @08:09:16.68 AS.reconfigure ASconfig @08:09:16.72 AS.seek 1ml @08:10:14.50 delay 1 second @08:10:15.52 AFV.select :holdingCoil @08:10:15.62 delay 1 second @08:10:16.66 AS.reconfigure ASfast @08:10:16.70 AS.seek 0ml @08:10:44.04 delay 3 seconds @08:10:47.07 AFV.select :carrierSolution @08:10:47.17 delay 2 seconds @08:10:49.21 AS.reconfigure ASconfig @08:10:49.26 AS.seek 1ml @08:11:47.04 delay 1 second @08:11:48.06 AFV.select :holdingCoil @08:11:48.16 delay 1 second @08:11:49.20 AS.reconfigure ASnorm @08:11:49.24 AS.seek 0ml @08:12:39.96 Setting up PCR for IPc -- writing /var/log/esp/IPc18sep0708h.pcr @08:12:39.97 Building PCR Slug from ASV with MM1 mix, PP1 primer @08:12:39.99 Priming with carrier @08:12:40.00 ATV.dial ARV @08:12:40.59 ARV.dial :carrier @08:12:40.97 AS.seek 0.5ml @08:13:06.54 AFV.select :carrierSolution @08:13:06.65 delay 2 seconds @08:13:08.69 AS.reconfigure ASconfig @08:13:08.74 AS.seek 1ml @08:13:37.75 delay 1 second @08:13:38.78 AFV.select :holdingCoil @08:13:38.88 delay 1 second @08:13:39.91 ATV.dial :waste @08:13:41.57 AS.reconfigure ASnorm @08:13:41.62 AS.seek :empty @08:14:32.34 AFV.select :carrierSolution @08:14:32.45 delay 2 seconds @08:14:34.49 AS.reconfigure ASconfig @08:14:34.54 AS.seek 0.5ml @08:15:03.65 delay 1 second @08:15:04.67 AFV.select :holdingCoil @08:15:04.78 Building Slug @08:15:04.79 ATV.dial :ARV @08:15:06.40 delay 2 seconds @08:15:08.43 ARV.dial :air1 @08:15:08.83 delay 2 seconds @08:15:10.91 AS.reconfigure ASslow2 @08:15:10.96 AS.seek 0.506ml @08:15:17.25 delay 2 seconds @08:15:19.28 ARV.dial :MM1 @08:15:19.90 delay 2 seconds @08:15:21.94 Adding MM1 @08:15:22.59 AS.seek 0.524ml @08:15:40.87 delay 25 seconds @08:16:05.90 ARV.dial :PP1 @08:16:06.30 Adding PP1 @08:16:06.31 delay 2 seconds @08:16:08.39 AS.seek 0.53ml @08:16:14.54 delay 10 seconds @08:16:24.57 ARV.dial :air1 @08:16:24.97 adding ARV air @08:16:24.98 delay 2 seconds @08:16:27.07 AS.seek 0.536ml @08:16:33.34 delay 5 seconds @08:16:38.37 ARV.dial :carrier @08:16:38.79 delay 7 seconds @08:16:45.87 AS.seek 0.594ml @08:17:44.76 delay 5 seconds @08:17:49.79 ATV.dial :ASV @08:17:50.20 delay 2 seconds @08:17:52.30 AS.seek 0.6ml @08:17:58.57 delay 4 seconds @08:18:02.59 ATV.dial ARV @08:18:03.14 delay 2 seconds @08:18:05.17 ARV.dial :carrier @08:18:05.19 delay 2 seconds @08:18:07.28 AS.seek 0.65ml @08:18:57.75 delay 4 seconds @08:19:01.78 ATV.dial PCR @08:19:02.39 ASV.dial :waste1 @08:19:02.41 ARV.dial :air1 @08:19:02.83 Positioning Slug by 0.324ml @08:19:02.92 AS.seek 0.326ml @08:24:28.71 delay 5 seconds @08:24:33.74 ATV.dial ARV @08:24:34.60 ASV.dial :PCRlock @08:24:34.98 delay 5 seconds @08:24:40.01 Running IPc ProfileIPc thermocycling @08:24:40.03 PCR.program ProfileIPc @08:24:40.18 ProfileIPc should complete in < 1:50:49 @08:24:40.20 PCR.run 2:23:31.25 @09:50:33.99 Session Begun @09:50:48.69 -> showlog 20 @09:51:20.99 -> can @09:51:46.88 PCR.off @09:51:49.54 PCR for IPc completed -- closed /var/log/esp/IPc18sep0708h.pcr @09:51:49.56 Full MFB Decontamination @09:51:49.59 Decon extraction column and decon AIV @09:51:49.63 SPE.hold 2:46:40,55C @09:51:49.70 ASV.dial :SPEdelivery @09:51:50.38 delay 20 seconds @09:52:10.41 AFV.select :carrierSolution @09:52:10.54 delay 2 seconds @09:52:12.58 AS.reconfigure ASconfig @09:52:12.65 AS.seek 0.5ml @09:52:22.97 delay 1 second @09:52:23.99 AFV.select :holdingCoil @09:52:24.10 ATV.dial :air1 @09:52:26.28 AS.reconfigure ASslow1 @09:52:26.36 AS.seek 0.52ml @09:52:30.71 ATV.dial :ASV @09:52:32.41 ASV.dial :SPErecovery @09:52:33.58 AS.seek 0.62ml @09:52:54.07 ATV.dial :waste @09:52:56.20 AS.reconfigure ASnorm @09:52:56.28 AS.seek :empty @09:53:27.94 AFV.select :holdingCoil @09:53:27.96 ATV.dial :waste @09:53:28.00 AS.reconfigure ASconfig @09:53:28.08 AS.seek 0ml @09:53:28.43 AFV.select :carrierSolution @09:53:28.56 delay 2 seconds @09:53:30.60 AS.seek 0.5ml @09:53:59.63 delay 1 second @09:54:00.66 AFV.select :holdingCoil @09:54:00.78 ATV.dial :air3 @09:54:01.54 AS.reconfigure ASslow1 @09:54:01.61 AS.seek 0.52ml @09:54:05.94 ATV.dial :air3 @09:54:06.06 AS.seek 0.53ml @09:54:08.36 delay 2 seconds @09:54:10.39 ATV.dial :bleach @09:54:10.91 AS.seek 0.71ml @09:54:47.41 delay 3 seconds @09:54:50.44 ATV.dial :air3 @09:54:51.04 delay 3 seconds @09:54:54.13 AS.seek 0.735ml @09:54:59.47 delay 3 seconds @09:55:02.50 ATV.dial :bleach @09:55:03.18 AS.seek 0.72ml @09:55:06.52 delay 3 seconds @09:55:09.55 ATV.dial ASV @09:55:10.68 ASV.dial :SPErecovery @09:55:10.79 AS.seek 0.365ml @09:56:22.47 ATV.dial :waste @09:56:24.41 AS.reconfigure ASnorm @09:56:24.48 AS.seek :empty @09:56:43.30 ATV.dial :air3 @09:56:44.04 AS.reconfigure ASslow1 @09:56:44.12 AS.seek 0.009ml @09:56:46.49 delay 2 seconds @09:56:48.52 ATV.dial :bleach @09:56:49.01 AS.seek 0.579ml @09:58:43.93 delay 3 seconds @09:58:46.96 ATV.dial :air3 @09:58:47.54 delay 3 seconds @09:58:50.63 AS.seek 0.604ml @09:58:55.86 delay 3 seconds @09:58:58.89 ATV.dial :bleach @09:58:59.54 AS.seek 0.589ml @09:59:02.86 delay 3 seconds @09:59:05.89 ATV.dial :ASV @09:59:06.96 ASV.dial :wasteR @09:59:07.47 AS.seek 0ml @10:01:06.25 delay 1:00 @10:02:06.28 ATV.dial ASV @10:02:06.29 AFV.select :carrierSolution @10:02:06.42 delay 2 seconds @10:02:08.46 AS.reconfigure ASconfig @10:02:08.53 AS.seek 1ml @10:03:06.30 delay 1 second @10:03:07.32 AFV.select :holdingCoil @10:03:07.45 delay 1 second @10:03:08.48 ASV.dial :wasteR @10:03:08.50 AS.reconfigure ASslow1 @10:03:08.59 AS.seek 0ml @10:06:29.99 delay 10 seconds @10:06:40.02 ATV.dial :air3 @10:06:41.58 AS.seek 0.01ml @10:06:43.93 delay 2 seconds @10:06:45.96 ATV.dial :bleach @10:06:46.62 AS.seek 0.58ml @10:08:41.54 delay 3 seconds @10:08:44.57 ATV.dial :air3 @10:08:45.14 delay 3 seconds @10:08:48.24 AS.seek 0.605ml @10:08:53.55 delay 3 seconds @10:08:56.58 ATV.dial :bleach @10:08:57.22 AS.seek 0.59ml @10:09:00.55 delay 3 seconds @10:09:03.58 ATV.dial ASV @10:09:04.64 ASV.dial :SPEdelivery @10:09:05.05 AS.seek 0ml @10:11:03.97 delay 1:00 @10:12:04.00 AFV.select :carrierSolution @10:12:04.13 delay 2 seconds @10:12:06.17 AS.reconfigure ASconfig @10:12:06.24 AS.seek 1ml @10:13:04.61 delay 1 second @10:13:05.64 AFV.select :holdingCoil @10:13:05.76 delay 1 second @10:13:06.79 ATV.dial ASV @10:13:06.80 ASV.dial :SPEdelivery @10:13:06.85 AS.reconfigure ASslow1 @10:13:06.93 AS.seek 0ml @10:16:28.33 delay 10 seconds @10:16:38.36 ATV.dial :air2 @10:16:39.22 AS.reconfigure ASslow0 @10:16:39.29 AS.seek 0.8ml @10:18:00.11 delay 3 seconds @10:18:03.14 ATV.dial :ASV @10:18:04.16 ASV.dial :SPEdelivery @10:18:04.19 AS.reconfigure ASslow1 @10:18:04.28 AS.seek 0.03ml @10:20:39.44 delay 20 seconds @10:20:59.47 ATV.dial :waste,via: :air3 @10:21:01.25 AS.reconfigure ASconfig @10:21:01.33 AS.seek :empty @10:21:03.41 SPE.coast @10:21:03.52 Removing Bleach from Mixing Coil @10:21:03.53 AFV.select :carrierSolution @10:21:03.67 delay 2 seconds @10:21:05.71 AS.seek 0.5ml @10:21:34.83 delay 1 second @10:21:35.86 AFV.select :holdingCoil @10:21:35.97 ATV.dial :ASV @10:21:37.69 ASV.dial :SPErecovery @10:21:38.36 AS.reconfigure ASslow1 @10:21:38.44 AS.seek 0.73ml @10:22:24.99 ASV.dial :wasteD @10:22:25.61 AS.reconfigure ASnorm @10:22:25.69 AS.seek 0ml @10:23:02.82 AFV.select :carrierSolution @10:23:02.95 delay 2 seconds @10:23:04.99 AS.reconfigure ASconfig @10:23:05.06 AS.seek 0.5ml @10:23:34.13 delay 1 second @10:23:35.16 AFV.select :holdingCoil @10:23:35.29 ATV.dial ASV @10:23:35.31 ASV.dial :SPErecovery @10:23:36.00 AS.reconfigure ASslow0 @10:23:36.08 AS.seek 0.3ml @10:23:56.52 ATV.dial :air2 @10:23:57.60 AS.reconfigure ASslow1 @10:23:57.67 AS.seek 0.32ml @10:24:02.02 ATV.dial ASV @10:24:03.18 AS.reconfigure ASslow0 @10:24:03.26 AS.seek 0.57ml @10:24:28.75 ASV.dial :wasteD @10:24:29.37 AS.reconfigure ASnorm @10:24:29.45 AS.seek 0ml @10:24:58.53 AFV.select :carrierSolution @10:24:58.66 delay 2 seconds @10:25:00.70 AS.reconfigure ASconfig @10:25:00.77 AS.seek 0.5ml @10:25:29.85 delay 1 second @10:25:30.88 AFV.select :holdingCoil @10:25:30.99 ATV.dial ASV @10:25:31.01 ASV.dial :SPErecovery @10:25:31.78 AS.reconfigure ASslow0 @10:25:31.85 AS.seek 0.3ml @10:25:52.30 ATV.dial :air2 @10:25:53.35 AS.reconfigure ASslow1 @10:25:53.43 AS.seek 0.32ml @10:25:57.80 ATV.dial ASV @10:25:58.85 AS.reconfigure ASslow0 @10:25:58.93 AS.seek 0.57ml @10:26:24.41 ASV.dial :wasteD @10:26:25.12 AS.reconfigure ASnorm @10:26:25.20 AS.seek 0ml @10:26:54.30 ATV.dial :air2 @10:26:55.29 AS.reconfigure ASslow0 @10:26:55.36 AS.seek 0.3ml @10:27:25.82 ATV.dial :ASV @10:27:26.80 ASV.dial :wasteD @10:27:26.83 AS.reconfigure ASconfig @10:27:26.91 AS.seek 0.05ml @10:27:41.61 ATV.dial :waste @10:27:43.59 AS.seek 0ml @10:27:46.75 ASV.dial :SPEdelivery @10:27:47.76 deconLysateINT @10:27:47.77 ATV.dial :air3 @10:27:48.49 AS.reconfigure ASslow1 @10:27:48.57 AS.seek 0.01ml @10:27:50.96 delay 2 seconds @10:27:52.99 ATV.dial :bleach @10:27:54.07 AS.seek 0.96ml @10:30:32.01 -> exit @10:30:32.03 Session Ended @10:31:05.50 delay 3 seconds @10:31:08.53 ATV.dial :air3 @10:31:09.11 delay 3 seconds @10:31:12.21 AS.seek 0.985ml @10:31:17.59 delay 3 seconds @10:31:20.61 ATV.dial :bleach @10:31:21.24 AS.seek 0.97ml @10:31:24.55 delay 3 seconds @10:31:27.57 ATV.dial AIV @10:31:28.82 AIV.dial :coilToMFB @10:31:28.84 AWV.select :waste @10:31:28.85 AFV.select :carrierSolution @10:31:28.96 delay 2 seconds @10:31:31.00 AS.reconfigure ASconfig @10:31:31.05 AS.seek 1ml @10:31:33.07 delay 1 second @10:31:34.09 AFV.select :holdingCoil @10:31:34.20 delay 1 second @10:31:35.24 AS.reconfigure ASslow0 @10:31:35.28 AS.seek :empty @10:33:16.25 AFV.select :carrierSolution @10:33:16.36 delay 2 seconds @10:33:18.40 AS.reconfigure ASconfig @10:33:18.44 AS.seek 1ml @10:34:16.25 delay 1 second @10:34:17.28 AFV.select :holdingCoil @10:34:17.38 delay 1 second @10:34:18.42 AS.reconfigure ASslow0 @10:34:18.46 AS.seek :empty @10:35:59.44 AFV.select :carrierSolution @10:35:59.55 delay 2 seconds @10:36:01.59 AS.reconfigure ASconfig @10:36:01.65 AS.seek 1ml @10:36:59.45 delay 1 second @10:37:00.48 AFV.select :holdingCoil @10:37:00.58 delay 1 second @10:37:01.62 AS.reconfigure ASslow0 @10:37:01.66 AS.seek :empty @10:38:42.61 ATV.dial :air2 @10:38:43.28 AS.reconfigure ASslow1 @10:38:43.34 AS.seek 0.009ml @10:38:45.68 AS.reconfigure ASslow0 @10:38:45.73 AS.seek 1ml @10:40:25.67 ATV.dial AIV @10:40:26.43 AS.seek 0.02ml @10:42:05.35 ATV.dial :waste @10:42:06.98 AS.seek 0ml @10:42:09.27 AWV.select :air @10:42:09.44 Cleaning PCR @10:42:09.45 AFV.select :carrierSolution @10:42:09.55 delay 2 seconds @10:42:11.59 AS.reconfigure ASconfig @10:42:11.63 AS.seek 0.5ml @10:42:40.66 delay 1 second @10:42:41.68 AFV.select :holdingCoil @10:42:41.78 ASV.dial :waste1 @10:42:42.14 ATV.dial :air3 @10:42:42.84 AS.reconfigure ASslow1 @10:42:42.90 AS.seek 0.51ml @10:42:45.17 delay 2 seconds @10:42:47.19 ATV.dial :bleach @10:42:47.66 AS.seek 0.81ml @10:43:48.27 delay 3 seconds @10:43:51.30 ATV.dial :air3 @10:43:51.85 delay 3 seconds @10:43:54.94 AS.seek 0.835ml @10:44:00.29 delay 3 seconds @10:44:03.32 ATV.dial :bleach @10:44:03.96 AS.seek 0.82ml @10:44:07.27 delay 3 seconds @10:44:10.30 ARV.dial :waste @10:44:10.91 delay 3 seconds @10:44:13.94 ATV.dial :ARV @10:44:14.82 AS.reconfigure ASconfig @10:44:14.88 AS.seek 0.47ml @10:44:35.30 AFV.select :carrierSolution @10:44:35.41 delay 2 seconds @10:44:37.45 AS.seek 1ml @10:45:08.24 delay 1 second @10:45:09.26 AFV.select :holdingCoil @10:45:09.36 delay 1 second @10:45:10.40 AS.reconfigure ASfast @10:45:10.44 AS.seek 0ml @10:45:20.82 Session Begun @10:45:37.80 AFV.select :carrierSolution @10:45:37.94 delay 2 seconds @10:45:39.98 AS.reconfigure ASconfig @10:45:40.05 AS.seek 1ml @10:46:37.85 delay 1 second @10:46:38.87 AFV.select :holdingCoil @10:46:38.99 delay 1 second @10:46:40.03 AS.reconfigure ASfast @10:46:40.10 AS.seek 0ml @10:47:07.47 AFV.select :carrierSolution @10:47:07.60 delay 2 seconds @10:47:09.64 AS.reconfigure ASconfig @10:47:09.71 AS.seek 1ml @10:48:07.52 delay 1 second @10:48:08.54 AFV.select :holdingCoil @10:48:09.23 delay 1 second @10:48:10.27 AS.reconfigure ASfast @10:48:10.34 AS.seek 0ml @10:48:37.69 ARV.dial between carrier and air1 @10:48:38.23 ATV.dial :air3 @10:48:39.52 AS.reconfigure ASslow1 @10:48:39.60 AS.seek 0.01ml @10:48:41.99 delay 2 seconds @10:48:44.02 ATV.dial :bleach @10:48:44.66 AS.seek 0.81ml @10:51:25.81 delay 3 seconds @10:51:28.84 ATV.dial :air3 @10:51:29.41 delay 3 seconds @10:51:32.50 AS.seek 0.835ml @10:51:37.84 delay 3 seconds @10:51:40.87 ATV.dial :bleach @10:51:41.52 AS.seek 0.82ml @10:51:44.82 delay 3 seconds @10:51:47.86 ATV.dial PCR @10:51:48.28 AS.reconfigure ASconfig @10:51:48.35 AS.seek :empty @10:52:35.83 PCR.program :bleachProfile @10:52:35.90 PCR program should complete in < 2:45 @10:52:35.92 PCR.run 10:00 @10:55:34.73 PCR.off @10:55:34.77 AFV.select :carrierSolution @10:55:34.90 delay 2 seconds @10:55:36.94 AS.seek 1ml @10:56:34.78 delay 1 second @10:56:35.81 AFV.select :holdingCoil @10:56:35.93 delay 1 second @10:56:36.97 AS.reconfigure ASfast @10:56:37.04 AS.seek 0ml @10:57:04.39 delay 3 seconds @10:57:07.42 AFV.select :carrierSolution @10:57:07.55 delay 2 seconds @10:57:09.59 AS.reconfigure ASconfig @10:57:09.67 AS.seek 1ml @10:58:07.48 delay 1 second @10:58:08.50 AFV.select :holdingCoil @10:58:08.63 delay 1 second @10:58:09.66 AS.reconfigure ASfast @10:58:09.73 AS.seek 0ml @10:58:37.09 delay 3 seconds @10:58:40.12 AFV.select :carrierSolution @10:58:40.24 delay 2 seconds @10:58:42.28 AS.reconfigure ASconfig @10:58:42.36 AS.seek 1ml @10:59:40.16 delay 1 second @10:59:41.19 AFV.select :holdingCoil @10:59:41.31 delay 1 second @10:59:42.35 AS.reconfigure ASfast @10:59:42.42 AS.seek 0ml @11:00:09.78 delay 3 seconds @11:00:12.81 AFV.select :carrierSolution @11:00:12.94 delay 2 seconds @11:00:14.98 AS.reconfigure ASconfig @11:00:15.06 AS.seek 1ml @11:01:12.84 delay 1 second @11:01:13.86 AFV.select :holdingCoil @11:01:13.99 delay 1 second @11:01:15.02 AS.reconfigure ASfast @11:01:15.09 AS.seek 0ml @11:01:42.45 delay 3 seconds @11:01:45.48 AFV.select :carrierSolution @11:01:45.60 delay 2 seconds @11:01:47.64 AS.reconfigure ASconfig @11:01:47.71 AS.seek 1ml @11:02:45.48 delay 1 second @11:02:46.51 AFV.select :holdingCoil @11:02:46.63 delay 1 second @11:02:47.67 AS.reconfigure ASnorm @11:02:47.74 AS.seek 0ml @11:03:38.46 AFV.select :carrierSolution @11:03:38.58 delay 2 seconds @11:03:40.62 AS.reconfigure ASconfig @11:03:40.68 AS.seek 1ml @11:04:38.50 delay 1 second @11:04:39.53 AFV.select :holdingCoil @11:04:39.65 delay 1 second @11:04:40.68 ATV.dial :waste @11:04:41.92 AS.reconfigure ASnorm @11:04:42.00 AS.seek 0.5ml @11:05:07.58 ATV.dial :waste2 @11:05:08.89 AS.seek 0ml @11:05:34.47
Asleep after completing 18tun assays @11:05:34.48 Mission runtime was 14:27:51.760132 @11:05:34.51 GoodNews.email "{1 => 0, 2 => 0, 3 => 2, 4 => 0, 5 => 0, 6 => 0, 7 => 0}\nCan@10:57:42, 24.5C, 69% humidity, 14.7psia, 12.051V, 0.500A, 0.500A avg, 6.02W",Subject: "Asleep after completing 18tun assays" @11:05:43.31 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log nohup: ESP: No such file or directory @22:24:19.17CEST07-Sep-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @22:24:21.60 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @22:24:21.91 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @22:24:27.33 Polling Can every 10:00 @22:24:29.15 SPE.coast @22:24:29.21 SPE.configure SPEconfig @22:24:29.31 AS.reconfigure ASconfig @22:24:29.35 AS.hold @22:24:29.41 AS.configure ASconfig @22:24:29.51 SS.reconfigure SSconfig @22:24:29.56 SS.hold @22:24:29.61 SS.configure SSconfig @22:24:29.72 CC.reconfigure CCconfig @22:24:29.76 CC.hold @22:24:29.82 CC.configure CCconfig @22:24:29.92 CS.reconfigure CSconfig @22:24:29.96 CS.hold @22:24:30.04 CS.configure CSconfig @22:24:30.52 PC.reconfigure PCconfig @22:24:30.56 PC.hold @22:24:30.62 PC.configure PCconfig @22:24:30.72 PS.reconfigure PSconfig @22:24:30.76 PS.hold @22:24:30.84 PS.configure PSconfig @22:24:30.96 Forearm.reconfigure LooseForearm @22:24:31.01 Forearm.hold @22:24:31.06 Forearm.configure LooseForearm @22:24:31.15 Elbow.reconfigure LooseElbow @22:24:31.20 Elbow.hold @22:24:31.25 Elbow.configure LooseElbow @22:24:31.36 SC.reconfigure SCloose @22:24:31.41 SC.hold @22:24:31.47 SC.configure SCloose @22:24:31.57 SE.reconfigure SEconfig @22:24:31.62 SE.hold @22:24:31.67 SE.configure SEconfig @22:24:31.72 CH.coast @22:24:31.77 CH.configure CHconfig @22:24:31.80 PH.coast @22:24:31.86 PH.configure PHconfig @22:24:31.90 PCR.off @22:24:31.93 PCR.pidtemp= 50,0.1,0,300,25,25 @22:24:31.96 PCR.caltemp= 2.7073,0.0373,0 @22:24:31.99 All dwarf controllers running firmware version 3.75 @22:24:32.13 Loading /home/esp/esp2/mission/18tun1.rb mission @22:24:32.20 Preparing to sleep until +0:00:15 @22:24:32.22 GoodNews.email "Starting 18tun1 w/pucks in tube 2\n{}\nCan@22:24:27, 23.9C, 49% humidity, 14.5psia, 11.957V, 0.960A, 0.960A avg, 11.48W",Subject: "18tun1 begun" @22:24:32.31 AS.coast @22:24:32.33
Asleep til +0:00:15 @22:24:32.38 Continuing at 22:24 @22:24:32.40 SS.coast @22:24:32.48 CC.coast @22:24:32.53 CS.coast @22:24:32.57 PC.coast @22:24:32.60 PS.coast @22:24:32.64 Forearm.coast @22:24:32.68 Elbow.coast @22:24:32.72 SC.coast @22:24:32.76 SE.coast @22:24:33.15 Gate.power :core,:OFF @22:24:33.16 Gate.power :raw,:OFF @22:24:33.17 Shutdown @22:24:40.04 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:106\n{}\nCan@22:24:32, 24.0C, 49% humidity, 14.5psia, 11.982V, 0.846A, 0.846A avg, 10.14W",Subject: "Asleep until 22:24" @22:24:47.16
Powering core bus @22:24:52.19 Gate.power :raw,:ON @22:24:52.84 SPE.coast @22:24:52.90 SPE.configure SPEconfig @22:24:52.99 AS.configure ASconfig @22:24:53.09 SS.configure SSconfig @22:24:53.19 CC.configure CCconfig @22:24:53.29 CS.configure CSconfig @22:24:53.39 PC.configure PCconfig @22:24:53.48 PS.configure PSconfig @22:24:53.60 Forearm.configure LooseForearm @22:24:53.69 Elbow.configure LooseElbow @22:24:53.81 SC.configure SCloose @22:24:53.91 SE.configure SEconfig @22:24:53.95 CH.coast @22:24:54.00 CH.configure CHconfig @22:24:54.04 PH.coast @22:24:54.10 PH.configure PHconfig @22:24:54.14 PCR.off @22:24:54.17 PCR.pidtemp= 50,0.1,0,300,25,25 @22:24:54.20 PCR.caltemp= 2.7073,0.0373,0 @22:24:54.23 All dwarf controllers running firmware version 3.75 @22:24:54.34 Valve.reset @22:24:54.51 PCR.off @22:24:54.55 PCR.pidtemp= 50,0.1,0,300,25,25 @22:24:54.58 PCR.caltemp= 2.7073,0.0373,0 @22:24:54.75 Intake.select :close @22:24:54.78 Exhaust.select :close @22:24:54.81 CTV.dial :air @22:24:54.91 PSV.dial :air @22:24:54.94 CSV.dial :waste1 @22:24:55.10
Forearm.reconfigure LooseForearm @22:24:55.16 Forearm.home.seek :retract @22:24:55.19 SSV.dial :spit,avoiding: :exhaust @22:24:55.77 CS.reconfigure CSfast @22:24:55.80 SS.reconfigure SSconfig @22:24:55.90 CS.home.seek 0ml @22:24:55.94 SS.home.seek 0ml @22:24:56.25 PS.reconfigure PSconfig @22:24:56.30 PS.home.seek 0ml @22:24:56.37 Hand.close @22:24:56.41
Elbow.jog 1500 @22:24:56.90 Elbow.reconfigure LooseElbow @22:24:56.94 Elbow.home.seek :clear @22:25:01.55 SE.reconfigure SEconfig @22:25:01.60 SE.home.seek :down @22:25:02.32 SC.reconfigure SCsloppy @22:25:02.37 SC.home.seek :initial @22:25:05.52 PS.coast @22:25:12.92
Forearm.seek :retract @22:25:13.00 Elbow.seek :hammer @22:25:14.10 Forearm.reconfigure TightForearm @22:25:14.15 Forearm.seek 5 + 350 counts @22:25:15.23 Forearm.seek :retract @22:25:16.44 PC.reconfigure PCconfig @22:25:16.45
CC.reconfigure CCconfig @22:25:16.56 CC.home.seek :open @22:25:16.58 PC.home.seek :open @22:25:18.25 Session Begun @22:25:19.99
Phase [mbaPhase] @22:25:20.00 GoodNews.email "At 22:25:19 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:106\n{}\nCan@22:25:19, 23.9C, 49% humidity, 14.5psia, 12.006V, 0.752A, 0.752A avg, 9.03W",Subject: "Started 18tun1 phase mbaPhase" @22:25:20.05CEST07-Sep-18
==================> STARTED MBAsuperphase (phase #1) <=== @22:25:20.07 Starting initialPurge @22:25:20.11 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:25:20.22 Hand.open @22:25:21.09 Elbow.reconfigure TightElbow @22:25:21.16 Elbow.seek FlushPuck::Garage @22:25:23.18 Elbow.reconfigure LooseElbow @22:25:23.38 Forearm.reconfigure LooseForearm @22:25:23.46 Forearm.seek :garage @22:25:24.52 Hand.close @22:25:25.47 Forearm.seek :retract @22:25:26.51 Elbow.reconfigure TightElbow @22:25:26.58 Elbow.seek CC @22:25:29.56 Elbow.reconfigure LooseElbow @22:25:29.69 Forearm.reconfigure TightForearm @22:25:29.77 Forearm.seek CC @22:25:31.10 CC.seek :closed @22:25:35.43 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.24 @22:25:35.55 Hand.open @22:25:36.38 Forearm.reconfigure LooseForearm @22:25:36.44 Forearm.seek :retract @22:25:37.46 Flushing the Collection Valve::Manifold @22:25:37.62 CSV.dial CSR @22:25:39.16 Pulling 5ml of CSR.flush @22:25:39.17 CSR.select :flush @22:25:39.33 CS.reconfigure CSconfig @22:25:39.40 CS.seek 5ml @22:25:53.23 delay 5 seconds @22:25:58.26 CSR.select :air @22:25:58.45 CS.reconfigure CSfast @22:25:58.52 CS.seek 7.501ml @22:26:05.97 CSV.dial :waste @22:26:07.46 CS.reconfigure CSconfig @22:26:07.54 CS.seek 0ml @22:26:27.63 Purging external Intake @22:26:27.78 CSV.dial :flush @22:26:28.32 CS.reconfigure CSfast @22:26:28.39 CS.seek 10ml @22:26:54.88 delay 5 seconds @22:26:59.91 CSV.dial CBV @22:26:59.95
CBV.dial CSV @22:27:00.67 -> exit @22:27:00.69 Session Ended @22:27:00.82
CTV.dial :intake @22:27:01.68 Intake.select :open @22:27:01.80 CS.seek 0ml @22:27:28.84 Intake.select :close @22:27:28.96 CTV.dial :air @22:27:29.81 Flushing upper manifold @22:27:29.94 CSV.dial :flush @22:27:30.77 CS.seek 5ml @22:27:44.54 delay 5 seconds @22:27:49.57 CSV.dial :air @22:27:50.14 CS.seek 7ml @22:27:56.20 CSV.dial CBV @22:27:56.22
CBV.dial CSV @22:27:57.39 CTV.dial :air @22:27:57.41 CS.seek 0ml @22:28:16.10 delay 7 seconds @22:28:23.14 CS.seek 3ml @22:28:31.82 CSV.dial :waste @22:28:32.33 CS.seek 0ml @22:28:41.10 CSV.dial :flush @22:28:42.02 CS.seek 3ml @22:28:50.67 delay 5 seconds @22:28:55.70 CSV.dial :air @22:28:56.27 CS.seek 5ml @22:29:02.48 CSV.dial :air @22:29:02.50 CS.seek 0ml @22:29:16.04 delay 7 seconds @22:29:23.08 CS.seek 3ml @22:29:31.67 CSV.dial :waste @22:29:32.50 CS.seek 0ml @22:29:41.07 Garaging FlushPuck in Collection Clamp @22:29:41.15 Elbow.reconfigure TightElbow @22:29:41.20 Elbow.seek CC @22:29:41.46 Elbow.reconfigure LooseElbow @22:29:41.56 Forearm.seek CC @22:29:42.55 Hand.close @22:29:43.31 CC.seek :open @22:29:47.70 Forearm.seek :retract @22:29:48.70 Elbow.reconfigure TightElbow @22:29:48.75 Elbow.seek :garage @22:29:51.93 Elbow.reconfigure LooseElbow @22:29:52.03 Forearm.reconfigure TightForearm @22:29:52.08 Forearm.seek :garage @22:29:53.25 Hand.open @22:29:54.13 Forearm.reconfigure LooseForearm @22:29:54.17 Forearm.seek :retract @22:29:55.28 Starting mfb @22:29:55.30 pcrBAC sampling at most 1500ml, wcr at most 1500ml @22:29:55.33 BACfans.email "Started mfb in tube 2 at 22:29:55CEST07-Sep-18\n{}\nCan@22:25:19, 23.9C, 49% humidity, 14.5psia, 12.006V, 0.752A, 0.752A avg, 9.03W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @22:29:55.41
Loading sh1 puck from tube 2 into Collection Clamp @22:29:55.53 Readying the MFB @22:29:55.59 Homing MFB Syringe @22:29:55.60 AFV.select :holdingCoil @22:29:55.84 ATV.dial :air2 @22:29:55.95
SC.reconfigure SCloose @22:29:56.18 Elbow.reconfigure TightElbow @22:29:56.23 Elbow.seek 2 @22:29:56.44 AS.reconfigure ASconfig @22:29:56.48 AS.home.seek :home @22:29:58.33
Forearm.reconfigure TightForearm @22:29:58.39 Forearm.seek 2 + 700 counts @22:29:59.48 SE.reconfigure SEpick @22:29:59.56 SE.seek beamBreak @22:30:02.00 AFV.select :carrierSolution @22:30:02.12 delay 2 seconds @22:30:02.54
SE.configure SEconfig @22:30:02.71 Elbow.seek 2 @22:30:03.11 Forearm.seek 2 + 300 counts @22:30:03.37 Forearm.reconfigure LooseForearm @22:30:03.42 Hand.close @22:30:04.18 AS.seek 1ml @22:30:04.25 SE.seek :down @22:30:04.52
Forearm.seek :retract @22:30:05.51 Elbow.seek CC @22:30:06.96 SE.coast @22:30:07.69
Elbow.reconfigure LooseElbow @22:30:07.82 Forearm.reconfigure TightForearm @22:30:07.87 Forearm.seek CC @22:30:09.27 CC.seek :closed @22:30:13.56 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.47 @22:30:13.57 Hand.open @22:30:14.39 Forearm.reconfigure LooseForearm @22:30:14.44 Forearm.seek :retract @22:30:15.45 SC.coast @22:30:15.49 collectSample 1500ml w/bubble point of 55psi @22:30:15.50 Priming 7ml @22:30:15.51 Gate.power :sampler,:ON @22:30:15.52 Intake.select :close @22:30:15.53 Exhaust.select :close @22:30:15.55 SS.hold @22:30:15.78 Calibrating pressure and temperature sensors @22:30:15.79 CTV.dial :air @22:30:15.81 SSV.dial :spit,avoiding: :exhaust @22:30:15.83 SS.reconfigure SSfast @22:30:15.87 SS.seek 5ml @22:30:19.02 SSV.dial CBV @22:30:19.05
CBV.dial SSV @22:30:20.60 delay 3.8 seconds @22:30:24.54 SSV.dial :spit @22:30:26.07 SS.seek 0ml @22:30:28.92 SSV.dial CBV @22:30:30.41 CTV.dial :intake @22:30:31.29 Waiting for external sampler to finish priming... @22:30:31.30 delayUntil "22:35:15.52" @22:30:57.16 delay 1 second @22:30:58.17 AFV.select :holdingCoil @22:30:58.28 delay 1 second @22:30:59.31 ATV.dial :waste @22:31:00.48 AS.reconfigure ASnorm @22:31:00.53 AS.home.seek :empty @22:31:53.11 ATV.dial :waste @22:31:53.13 ARV.dial between carrier and air1 @22:31:53.21 ASV.dial :waste1 @22:35:15.54 SSV.dial CBV @22:35:15.57
CBV.dial SSV @22:35:15.58 Allowing 40s for 7.0ml of remaining 7.0ml @22:35:15.59 Intake.select :open @22:35:17.76 Intake @29.4psia, vacuum from 20.0 to 24.0psi @22:35:17.78 SS.reconfigure SSinhale.with maxSpeed: 150 @22:35:17.83 SS.seek 7ml @22:35:23.34 Stroke completed -- waiting for pressure to equalize ... @22:35:30.29 Intake.select :close @22:35:30.44 SSV.dial :exhaust @22:35:31.29 Exhaust.select :open @22:35:31.40 SS.reconfigure SSexhale @22:35:31.46 SS.seek 0ml @22:35:35.37 Exhaust.select :close @22:35:35.50 Sampling 1500.0ml @22:35:35.52 Intake.select :close @22:35:35.54 Exhaust.select :close @22:35:35.55 SS.reconfigure SSconfig @22:35:35.60 SS.hold @22:35:35.81 CTV.dial :intake @22:35:35.82 SSV.dial CBV @22:35:35.85
CBV.dial SSV @22:35:36.73 Allowing 130s for 25.0ml of remaining 1500.0ml @22:35:36.74 Intake.select :open @22:35:38.91 Intake @29.9psia, vacuum from 20.0 to 24.0psi @22:35:38.93 SS.reconfigure SSinhale.with maxSpeed: 150 @22:35:38.97 SS.seek 25ml @22:35:42.05 Vacuum too high -- waiting up to 40s @22:35:43.12 Intake @27.1psia, vacuum from 20.0 to 24.0psi @22:35:43.62 SS.reconfigure SSinhale.with maxSpeed: 135 @22:35:43.69 SS.seek 25ml @22:35:48.11 Vacuum too high -- waiting up to 40s @22:35:50.70 Intake @27.8psia, vacuum from 20.0 to 24.0psi @22:35:50.71 SS.reconfigure SSinhale.with maxSpeed: 122 @22:35:50.76 SS.seek 25ml @22:35:53.69 Vacuum too high -- waiting up to 40s @22:35:55.51 Intake @27.9psia, vacuum from 20.0 to 24.0psi @22:35:55.53 SS.reconfigure SSinhale.with maxSpeed: 110 @22:35:55.58 SS.seek 25ml @22:35:58.68 Vacuum too high -- waiting up to 40s @22:36:00.50 Intake @28.3psia, vacuum from 20.0 to 24.0psi @22:36:00.52 SS.reconfigure SSinhale.with maxSpeed: 99 @22:36:00.56 SS.seek 25ml @22:36:03.64 Vacuum too high -- waiting up to 40s @22:36:05.22 Intake @28.2psia, vacuum from 20.0 to 24.0psi @22:36:05.24 SS.reconfigure SSinhale.with maxSpeed: 89 @22:36:05.28 SS.seek 25ml @22:36:08.84 Vacuum too high -- waiting up to 40s @22:36:10.41 Intake @27.8psia, vacuum from 20.0 to 24.0psi @22:36:10.43 SS.reconfigure SSinhale.with maxSpeed: 80 @22:36:10.48 SS.seek 25ml @22:36:12.95 Stroke completed -- waiting for pressure to equalize ... @22:36:14.89 Intake.select :close @22:36:15.05 SSV.dial :exhaust @22:36:15.91 Exhaust.select :open @22:36:16.02 SS.reconfigure SSexhale @22:36:16.07 SS.seek 0ml @22:36:24.95 Exhaust.select :close @22:36:25.08 SSV.dial CBV @22:36:25.10
CBV.dial SSV @22:36:25.99 Allowing 130s for 25.0ml of remaining 1475.0ml @22:36:26.00 Intake.select :open @22:36:28.19 Intake @29.9psia, vacuum from 20.0 to 24.0psi @22:36:28.21 SS.reconfigure SSinhale.with maxSpeed: 80 @22:36:28.25 SS.seek 25ml @22:36:32.14 Vacuum too high -- waiting up to 40s @22:36:32.97 Intake @27.6psia, vacuum from 20.0 to 24.0psi @22:36:33.49 SS.reconfigure SSinhale.with maxSpeed: 72 @22:36:33.55 SS.seek 25ml @22:36:40.81 Vacuum too high -- waiting up to 40s @22:36:42.40 Intake @28.0psia, vacuum from 20.0 to 24.0psi @22:36:42.41 SS.reconfigure SSinhale.with maxSpeed: 65 @22:36:42.46 SS.seek 25ml @22:36:50.33 Vacuum too high -- waiting up to 40s @22:36:51.65 Intake @27.9psia, vacuum from 20.0 to 24.0psi @22:36:51.67 SS.reconfigure SSinhale.with maxSpeed: 59 @22:36:51.72 SS.seek 25ml @22:37:03.06 Stroke completed -- waiting for pressure to equalize ... @22:37:05.63 Intake.select :close @22:37:05.79 SSV.dial :exhaust @22:37:06.66 Exhaust.select :open @22:37:06.77 SS.reconfigure SSexhale @22:37:06.82 SS.seek 0ml @22:37:16.27 Exhaust.select :close @22:37:16.38 SSV.dial CBV @22:37:16.41
CBV.dial SSV @22:37:17.30 Allowing 130s for 25.0ml of remaining 1450.0ml @22:37:17.31 Intake.select :open @22:37:19.49 Intake @29.9psia, vacuum from 20.0 to 24.0psi @22:37:19.50 SS.reconfigure SSinhale.with maxSpeed: 59 @22:37:19.55 SS.seek 25ml @22:37:25.05 Vacuum too high -- waiting up to 40s @22:37:25.62 Intake @27.9psia, vacuum from 20.0 to 24.0psi @22:37:25.64 SS.reconfigure SSinhale.with maxSpeed: 53 @22:37:25.68 SS.seek 25ml @22:37:53.92 Stroke completed -- waiting for pressure to equalize ... @22:37:56.50 Intake.select :close @22:37:56.66 SSV.dial :exhaust @22:37:57.52 Exhaust.select :open @22:37:57.63 SS.reconfigure SSexhale @22:37:57.68 SS.seek 0ml @22:38:07.20 Exhaust.select :close @22:38:07.32 SSV.dial CBV @22:38:07.35
CBV.dial SSV @22:38:08.22 Allowing 130s for 25.0ml of remaining 1425.0ml @22:38:08.23 Intake.select :open @22:38:10.40 Intake @29.7psia, vacuum from 20.0 to 24.0psi @22:38:10.42 SS.reconfigure SSinhale.with maxSpeed: 53 @22:38:10.47 SS.seek 25ml @22:38:20.42 Vacuum too high -- waiting up to 40s @22:38:21.24 Intake @28.4psia, vacuum from 20.0 to 24.0psi @22:38:21.26 SS.reconfigure SSinhale.with maxSpeed: 48 @22:38:21.31 SS.seek 25ml @22:38:47.68 Stroke completed -- waiting for pressure to equalize ... @22:38:49.63 Intake.select :close @22:38:49.78 SSV.dial :exhaust @22:38:50.65 Exhaust.select :open @22:38:50.76 SS.reconfigure SSexhale @22:38:50.81 SS.seek 0ml @22:39:00.32 Exhaust.select :close @22:39:00.44 SSV.dial CBV @22:39:00.48
CBV.dial SSV @22:39:01.35 Allowing 130s for 25.0ml of remaining 1400.0ml @22:39:01.36 Intake.select :open @22:39:03.53 Intake @29.9psia, vacuum from 20.0 to 24.0psi @22:39:03.55 SS.reconfigure SSinhale.with maxSpeed: 48 @22:39:03.59 SS.seek 25ml @22:39:22.42 Vacuum too high -- waiting up to 40s @22:39:22.99 Intake @27.9psia, vacuum from 20.0 to 24.0psi @22:39:23.01 SS.reconfigure SSinhale.with maxSpeed: 43 @22:39:23.06 SS.seek 25ml @22:39:44.52 Stroke completed -- waiting for pressure to equalize ... @22:39:45.85 Intake.select :close @22:39:46.01 SSV.dial :exhaust @22:39:46.88 Exhaust.select :open @22:39:46.99 SS.reconfigure SSexhale @22:39:47.04 SS.seek 0ml @22:39:56.42 Exhaust.select :close @22:39:56.54 SSV.dial CBV @22:39:56.57
CBV.dial SSV @22:39:57.47 Allowing 130s for 25.0ml of remaining 1375.0ml @22:39:57.48 Intake.select :open @22:39:59.66 Intake @30.0psia, vacuum from 20.0 to 24.0psi @22:39:59.67 SS.reconfigure SSinhale.with maxSpeed: 43 @22:39:59.73 SS.seek 25ml @22:40:41.21 Stroke completed -- waiting for pressure to equalize ... @22:40:43.16 Intake.select :close @22:40:43.32 SSV.dial :exhaust @22:40:44.19 Exhaust.select :open @22:40:44.30 SS.reconfigure SSexhale @22:40:44.35 SS.seek 0ml @22:40:53.57 Exhaust.select :close @22:40:53.69 SSV.dial CBV @22:40:53.72
CBV.dial SSV @22:40:54.61 Allowing 130s for 25.0ml of remaining 1350.0ml @22:40:54.62 Intake.select :open @22:40:56.79 Intake @30.0psia, vacuum from 20.0 to 24.0psi @22:40:56.81 SS.reconfigure SSinhale.with maxSpeed: 43 @22:40:56.86 SS.seek 25ml @22:41:38.26 Stroke completed -- waiting for pressure to equalize ... @22:41:40.20 Intake.select :close @22:41:40.36 SSV.dial :exhaust @22:41:41.22 Exhaust.select :open @22:41:41.33 SS.reconfigure SSexhale @22:41:41.38 SS.seek 0ml @22:41:50.76 Exhaust.select :close @22:41:50.88 SSV.dial CBV @22:41:50.91
CBV.dial SSV @22:41:51.79 Allowing 130s for 25.0ml of remaining 1325.0ml @22:41:51.80 Intake.select :open @22:41:53.98 Intake @29.9psia, vacuum from 20.0 to 24.0psi @22:41:53.99 SS.reconfigure SSinhale.with maxSpeed: 43 @22:41:54.04 SS.seek 25ml @22:42:35.37 Stroke completed -- waiting for pressure to equalize ... @22:42:37.96 Intake.select :close @22:42:38.12 SSV.dial :exhaust @22:42:38.99 Exhaust.select :open @22:42:39.10 SS.reconfigure SSexhale @22:42:39.15 SS.seek 0ml @22:42:48.96 Exhaust.select :close @22:42:49.07 SSV.dial CBV @22:42:49.10
CBV.dial SSV @22:42:50.00 Allowing 130s for 25.0ml of remaining 1300.0ml @22:42:50.01 Intake.select :open @22:42:52.18 Intake @29.9psia, vacuum from 20.0 to 24.0psi @22:42:52.20 SS.reconfigure SSinhale.with maxSpeed: 43 @22:42:52.25 SS.seek 25ml @22:43:15.52 Vacuum too high -- waiting up to 40s @22:43:16.09 Intake @28.2psia, vacuum from 20.0 to 24.0psi @22:43:16.10 SS.reconfigure SSinhale.with maxSpeed: 39 @22:43:16.15 SS.seek 25ml @22:43:36.59 Stroke completed -- waiting for pressure to equalize ... @22:43:38.54 Intake.select :close @22:43:38.70 SSV.dial :exhaust @22:43:39.57 Exhaust.select :open @22:43:39.69 SS.reconfigure SSexhale @22:43:39.73 SS.seek 0ml @22:43:49.01 Exhaust.select :close @22:43:49.13 SSV.dial CBV @22:43:49.16
CBV.dial SSV @22:43:50.05 Allowing 130s for 25.0ml of remaining 1275.0ml @22:43:50.06 Intake.select :open @22:43:52.23 Intake @29.8psia, vacuum from 20.0 to 24.0psi @22:43:52.25 SS.reconfigure SSinhale.with maxSpeed: 39 @22:43:52.29 SS.seek 25ml @22:44:37.87 Stroke completed -- waiting for pressure to equalize ... @22:44:39.81 Intake.select :close @22:44:39.97 SSV.dial :exhaust @22:44:40.83 Exhaust.select :open @22:44:40.95 SS.reconfigure SSexhale @22:44:40.99 SS.seek 0ml @22:44:50.99 Exhaust.select :close @22:44:51.10 SSV.dial CBV @22:44:51.13
CBV.dial SSV @22:44:52.02 Allowing 130s for 25.0ml of remaining 1250.0ml @22:44:52.03 Intake.select :open @22:44:54.21 Intake @29.9psia, vacuum from 20.0 to 24.0psi @22:44:54.22 SS.reconfigure SSinhale.with maxSpeed: 39 @22:44:54.28 SS.seek 25ml @22:45:39.56 Stroke completed -- waiting for pressure to equalize ... @22:45:42.15 Intake.select :close @22:45:42.31 SSV.dial :exhaust @22:45:43.17 Exhaust.select :open @22:45:43.28 SS.reconfigure SSexhale @22:45:43.33 SS.seek 0ml @22:45:52.32 Exhaust.select :close @22:45:52.43 SSV.dial CBV @22:45:52.46
CBV.dial SSV @22:45:53.36 Allowing 130s for 25.0ml of remaining 1225.0ml @22:45:53.37 Intake.select :open @22:45:55.54 Intake @29.9psia, vacuum from 20.0 to 24.0psi @22:45:55.56 SS.reconfigure SSinhale.with maxSpeed: 39 @22:45:55.61 SS.seek 25ml @22:46:41.79 Stroke completed -- waiting for pressure to equalize ... @22:46:44.37 Intake.select :close @22:46:44.53 SSV.dial :exhaust @22:46:45.39 Exhaust.select :open @22:46:45.51 SS.reconfigure SSexhale @22:46:45.55 SS.seek 0ml @22:46:54.96 Exhaust.select :close @22:46:55.08 SSV.dial CBV @22:46:55.11
CBV.dial SSV @22:46:56.00 Allowing 130s for 25.0ml of remaining 1200.0ml @22:46:56.01 Intake.select :open @22:46:58.19 Intake @30.1psia, vacuum from 20.0 to 24.0psi @22:46:58.20 SS.reconfigure SSinhale.with maxSpeed: 39 @22:46:58.25 SS.seek 25ml @22:47:43.60 Stroke completed -- waiting for pressure to equalize ... @22:47:46.18 Intake.select :close @22:47:46.34 SSV.dial :exhaust @22:47:47.21 Exhaust.select :open @22:47:47.32 SS.reconfigure SSexhale @22:47:47.37 SS.seek 0ml @22:47:56.78 Exhaust.select :close @22:47:56.89 SSV.dial CBV @22:47:56.92
CBV.dial SSV @22:47:57.80 Allowing 130s for 25.0ml of remaining 1175.0ml @22:47:57.81 Intake.select :open @22:47:59.99 Intake @29.9psia, vacuum from 20.0 to 24.0psi @22:48:00.01 SS.reconfigure SSinhale.with maxSpeed: 39 @22:48:00.05 SS.seek 25ml @22:48:42.51 Vacuum too high -- waiting up to 40s @22:48:43.34 Intake @28.6psia, vacuum from 20.0 to 24.0psi @22:48:43.35 SS.reconfigure SSinhale.with maxSpeed: 35 @22:48:43.40 SS.seek 25ml @22:48:46.87 Stroke completed -- waiting for pressure to equalize ... @22:48:48.82 Intake.select :close @22:48:48.99 SSV.dial :exhaust @22:48:49.86 Exhaust.select :open @22:48:49.97 SS.reconfigure SSexhale @22:48:50.03 SS.seek 0ml @22:48:59.09 Exhaust.select :close @22:48:59.21 SSV.dial CBV @22:48:59.24
CBV.dial SSV @22:49:00.14 Allowing 130s for 25.0ml of remaining 1150.0ml @22:49:00.15 Intake.select :open @22:49:02.33 Intake @30.0psia, vacuum from 20.0 to 24.0psi @22:49:02.35 SS.reconfigure SSinhale.with maxSpeed: 35 @22:49:02.39 SS.seek 25ml @22:49:54.08 Stroke completed -- waiting for pressure to equalize ... @22:49:56.69 Intake.select :close @22:49:56.84 SSV.dial :exhaust @22:49:57.70 Exhaust.select :open @22:49:57.81 SS.reconfigure SSexhale @22:49:57.87 SS.seek 0ml @22:50:06.90 Exhaust.select :close @22:50:07.02 SSV.dial CBV @22:50:07.05
CBV.dial SSV @22:50:07.95 Allowing 130s for 25.0ml of remaining 1125.0ml @22:50:07.96 Intake.select :open @22:50:10.14 Intake @29.8psia, vacuum from 20.0 to 24.0psi @22:50:10.15 SS.reconfigure SSinhale.with maxSpeed: 35 @22:50:10.20 SS.seek 25ml @22:51:01.38 Stroke completed -- waiting for pressure to equalize ... @22:51:03.95 Intake.select :close @22:51:04.11 SSV.dial :exhaust @22:51:04.97 Exhaust.select :open @22:51:05.08 SS.reconfigure SSexhale @22:51:05.13 SS.seek 0ml @22:51:14.77 Exhaust.select :close @22:51:14.89 SSV.dial CBV @22:51:14.92
CBV.dial SSV @22:51:15.80 Allowing 130s for 25.0ml of remaining 1100.0ml @22:51:15.81 Intake.select :open @22:51:17.99 Intake @30.0psia, vacuum from 20.0 to 24.0psi @22:51:18.01 SS.reconfigure SSinhale.with maxSpeed: 35 @22:51:18.06 SS.seek 25ml @22:52:08.41 Stroke completed -- waiting for pressure to equalize ... @22:52:10.99 Intake.select :close @22:52:11.15 SSV.dial :exhaust @22:52:12.02 Exhaust.select :open @22:52:12.13 SS.reconfigure SSexhale @22:52:12.19 SS.seek 0ml @22:52:21.62 Exhaust.select :close @22:52:21.74 SSV.dial CBV @22:52:21.77
CBV.dial SSV @22:52:22.67 Allowing 130s for 25.0ml of remaining 1075.0ml @22:52:22.68 Intake.select :open @22:52:24.85 Intake @30.0psia, vacuum from 20.0 to 24.0psi @22:52:24.87 SS.reconfigure SSinhale.with maxSpeed: 35 @22:52:24.92 SS.seek 25ml @22:53:15.30 Stroke completed -- waiting for pressure to equalize ... @22:53:17.86 Intake.select :close @22:53:18.02 SSV.dial :exhaust @22:53:18.89 Exhaust.select :open @22:53:19.00 SS.reconfigure SSexhale @22:53:19.05 SS.seek 0ml @22:53:28.50 Exhaust.select :close @22:53:28.61 SSV.dial CBV @22:53:28.64
CBV.dial SSV @22:53:29.53 Allowing 130s for 25.0ml of remaining 1050.0ml @22:53:29.54 Intake.select :open @22:53:31.71 Intake @30.0psia, vacuum from 20.0 to 24.0psi @22:53:31.73 SS.reconfigure SSinhale.with maxSpeed: 35 @22:53:31.78 SS.seek 25ml @22:54:22.13 Stroke completed -- waiting for pressure to equalize ... @22:54:24.70 Intake.select :close @22:54:24.85 SSV.dial :exhaust @22:54:25.72 Exhaust.select :open @22:54:25.83 SS.reconfigure SSexhale @22:54:26.36 SS.seek 0ml @22:54:35.96 Exhaust.select :close @22:54:36.08 SSV.dial CBV @22:54:36.11
CBV.dial SSV @22:54:37.00 Allowing 130s for 25.0ml of remaining 1025.0ml @22:54:37.01 Intake.select :open @22:54:39.19 Intake @30.0psia, vacuum from 20.0 to 24.0psi @22:54:39.20 SS.reconfigure SSinhale.with maxSpeed: 35 @22:54:39.25 SS.seek 25ml @22:55:29.49 Stroke completed -- waiting for pressure to equalize ... @22:55:32.09 Intake.select :close @22:55:32.25 SSV.dial :exhaust @22:55:33.12 Exhaust.select :open @22:55:33.23 SS.reconfigure SSexhale @22:55:33.28 SS.seek 0ml @22:55:42.25 Exhaust.select :close @22:55:42.38 SSV.dial CBV @22:55:42.40
CBV.dial SSV @22:55:43.30 Allowing 130s for 25.0ml of remaining 1000.0ml @22:55:43.31 Intake.select :open @22:55:45.49 Intake @29.7psia, vacuum from 20.0 to 24.0psi @22:55:45.50 SS.reconfigure SSinhale.with maxSpeed: 35 @22:55:45.55 SS.seek 25ml @22:56:36.89 Stroke completed -- waiting for pressure to equalize ... @22:56:39.47 Intake.select :close @22:56:39.63 SSV.dial :exhaust @22:56:40.50 Exhaust.select :open @22:56:40.61 SS.reconfigure SSexhale @22:56:40.66 SS.seek 0ml @22:56:50.33 Exhaust.select :close @22:56:50.45 SSV.dial CBV @22:56:50.48
CBV.dial SSV @22:56:51.37 Allowing 130s for 25.0ml of remaining 975.0ml @22:56:51.38 Intake.select :open @22:56:53.55 Intake @29.9psia, vacuum from 20.0 to 24.0psi @22:56:53.57 SS.reconfigure SSinhale.with maxSpeed: 35 @22:56:53.62 SS.seek 25ml @22:57:35.92 Vacuum too high -- waiting up to 40s @22:57:36.75 Intake @28.7psia, vacuum from 20.0 to 24.0psi @22:57:36.76 SS.reconfigure SSinhale.with maxSpeed: 32 @22:57:36.81 SS.seek 25ml @22:57:46.00 Stroke completed -- waiting for pressure to equalize ... @22:57:47.94 Intake.select :close @22:57:48.09 SSV.dial :exhaust @22:57:48.95 Exhaust.select :open @22:57:49.06 SS.reconfigure SSexhale @22:57:49.11 SS.seek 0ml @22:57:58.66 Exhaust.select :close @22:57:58.79 SSV.dial CBV @22:57:58.81
CBV.dial SSV @22:57:59.69 Allowing 130s for 25.0ml of remaining 950.0ml @22:57:59.70 Intake.select :open @22:58:01.87 Intake @30.0psia, vacuum from 20.0 to 24.0psi @22:58:01.89 SS.reconfigure SSinhale.with maxSpeed: 32 @22:58:01.94 SS.seek 25ml @22:58:56.78 Stroke completed -- waiting for pressure to equalize ... @22:58:59.36 Intake.select :close @22:58:59.52 SSV.dial :exhaust @22:59:00.39 Exhaust.select :open @22:59:00.51 SS.reconfigure SSexhale @22:59:00.55 SS.seek 0ml @22:59:09.89 Exhaust.select :close @22:59:10.01 SSV.dial CBV @22:59:10.04
CBV.dial SSV @22:59:11.39 Allowing 130s for 25.0ml of remaining 925.0ml @22:59:11.40 Intake.select :open @22:59:13.58 Intake @29.8psia, vacuum from 20.0 to 24.0psi @22:59:13.60 SS.reconfigure SSinhale.with maxSpeed: 32 @22:59:13.65 SS.seek 25ml @23:00:08.67 Stroke completed -- waiting for pressure to equalize ... @23:00:11.25 Intake.select :close @23:00:11.41 SSV.dial :exhaust @23:00:12.26 Exhaust.select :open @23:00:12.37 SS.reconfigure SSexhale @23:00:12.42 SS.seek 0ml @23:00:21.75 Exhaust.select :close @23:00:21.87 SSV.dial CBV @23:00:21.90
CBV.dial SSV @23:00:22.78 Allowing 130s for 25.0ml of remaining 900.0ml @23:00:22.79 Intake.select :open @23:00:24.96 Intake @29.9psia, vacuum from 20.0 to 24.0psi @23:00:24.98 SS.reconfigure SSinhale.with maxSpeed: 32 @23:00:25.03 SS.seek 25ml @23:01:20.28 Stroke completed -- waiting for pressure to equalize ... @23:01:22.87 Intake.select :close @23:01:23.03 SSV.dial :exhaust @23:01:23.90 Exhaust.select :open @23:01:24.01 SS.reconfigure SSexhale @23:01:24.06 SS.seek 0ml @23:01:33.56 Exhaust.select :close @23:01:33.68 SSV.dial CBV @23:01:33.71
CBV.dial SSV @23:01:34.59 Allowing 130s for 25.0ml of remaining 875.0ml @23:01:34.60 Intake.select :open @23:01:36.77 Intake @29.9psia, vacuum from 20.0 to 24.0psi @23:01:36.79 SS.reconfigure SSinhale.with maxSpeed: 32 @23:01:36.84 SS.seek 25ml @23:02:31.72 Stroke completed -- waiting for pressure to equalize ... @23:02:34.30 Intake.select :close @23:02:34.46 SSV.dial :exhaust @23:02:35.32 Exhaust.select :open @23:02:35.44 SS.reconfigure SSexhale @23:02:35.48 SS.seek 0ml @23:02:45.16 Exhaust.select :close @23:02:45.28 SSV.dial CBV @23:02:45.31
CBV.dial SSV @23:02:46.20 Allowing 130s for 25.0ml of remaining 850.0ml @23:02:46.21 Intake.select :open @23:02:48.39 Intake @29.6psia, vacuum from 20.0 to 24.0psi @23:02:48.40 SS.reconfigure SSinhale.with maxSpeed: 32 @23:02:48.45 SS.seek 25ml @23:03:43.81 Stroke completed -- waiting for pressure to equalize ... @23:03:46.38 Intake.select :close @23:03:46.54 SSV.dial :exhaust @23:03:47.40 Exhaust.select :open @23:03:47.51 SS.reconfigure SSexhale @23:03:47.56 SS.seek 0ml @23:03:56.97 Exhaust.select :close @23:03:57.09 SSV.dial CBV @23:03:57.12
CBV.dial SSV @23:03:58.01 Allowing 130s for 25.0ml of remaining 825.0ml @23:03:58.02 Intake.select :open @23:04:00.19 Intake @29.7psia, vacuum from 20.0 to 24.0psi @23:04:00.21 SS.reconfigure SSinhale.with maxSpeed: 32 @23:04:00.26 SS.seek 25ml @23:04:55.28 Stroke completed -- waiting for pressure to equalize ... @23:04:58.97 Intake.select :close @23:04:59.13 SSV.dial :exhaust @23:05:00.00 Exhaust.select :open @23:05:00.12 SS.reconfigure SSexhale @23:05:00.16 SS.seek 0ml @23:05:09.62 Exhaust.select :close @23:05:09.73 SSV.dial CBV @23:05:09.76
CBV.dial SSV @23:05:10.64 Allowing 130s for 25.0ml of remaining 800.0ml @23:05:10.65 Intake.select :open @23:05:12.82 Intake @30.0psia, vacuum from 20.0 to 24.0psi @23:05:12.84 SS.reconfigure SSinhale.with maxSpeed: 32 @23:05:12.89 SS.seek 25ml @23:06:08.08 Stroke completed -- waiting for pressure to equalize ... @23:06:10.65 Intake.select :close @23:06:10.81 SSV.dial :exhaust @23:06:11.67 Exhaust.select :open @23:06:11.78 SS.reconfigure SSexhale @23:06:11.83 SS.seek 0ml @23:06:21.28 Exhaust.select :close @23:06:21.39 SSV.dial CBV @23:06:21.42
CBV.dial SSV @23:06:22.31 Allowing 130s for 25.0ml of remaining 775.0ml @23:06:22.32 Intake.select :open @23:06:24.49 Intake @29.8psia, vacuum from 20.0 to 24.0psi @23:06:24.51 SS.reconfigure SSinhale.with maxSpeed: 32 @23:06:24.56 SS.seek 25ml @23:07:19.66 Stroke completed -- waiting for pressure to equalize ... @23:07:22.86 Intake.select :close @23:07:23.02 SSV.dial :exhaust @23:07:23.89 Exhaust.select :open @23:07:24.00 SS.reconfigure SSexhale @23:07:24.06 SS.seek 0ml @23:07:33.66 Exhaust.select :close @23:07:33.78 SSV.dial CBV @23:07:33.81
CBV.dial SSV @23:07:34.71 Allowing 130s for 25.0ml of remaining 750.0ml @23:07:34.72 Intake.select :open @23:07:36.89 Intake @29.7psia, vacuum from 20.0 to 24.0psi @23:07:36.91 SS.reconfigure SSinhale.with maxSpeed: 32 @23:07:36.95 SS.seek 25ml @23:08:14.82 Vacuum too high -- waiting up to 40s @23:08:15.64 Intake @28.6psia, vacuum from 20.0 to 24.0psi @23:08:15.66 SS.reconfigure SSinhale.with maxSpeed: 29 @23:08:15.71 SS.seek 25ml @23:08:34.84 Stroke completed -- waiting for pressure to equalize ... @23:08:37.40 Intake.select :close @23:08:37.56 SSV.dial :exhaust @23:08:38.43 Exhaust.select :open @23:08:38.54 SS.reconfigure SSexhale @23:08:38.60 SS.seek 0ml @23:08:48.23 Exhaust.select :close @23:08:48.35 SSV.dial CBV @23:08:48.38
CBV.dial SSV @23:08:49.26 Allowing 130s for 25.0ml of remaining 725.0ml @23:08:49.27 Intake.select :open @23:08:51.44 Intake @29.8psia, vacuum from 20.0 to 24.0psi @23:08:51.46 SS.reconfigure SSinhale.with maxSpeed: 29 @23:08:51.51 SS.seek 25ml @23:09:52.50 Stroke completed -- waiting for pressure to equalize ... @23:09:55.07 Intake.select :close @23:09:55.70 SSV.dial :exhaust @23:09:56.57 Exhaust.select :open @23:09:56.68 SS.reconfigure SSexhale @23:09:56.73 SS.seek 0ml @23:10:06.06 Exhaust.select :close @23:10:06.18 SSV.dial CBV @23:10:06.20
CBV.dial SSV @23:10:07.10 Allowing 130s for 25.0ml of remaining 700.0ml @23:10:07.11 Intake.select :open @23:10:09.29 Intake @29.8psia, vacuum from 20.0 to 24.0psi @23:10:09.30 SS.reconfigure SSinhale.with maxSpeed: 29 @23:10:09.35 SS.seek 25ml @23:11:10.73 Stroke completed -- waiting for pressure to equalize ... @23:11:13.29 Intake.select :close @23:11:13.45 SSV.dial :exhaust @23:11:14.32 Exhaust.select :open @23:11:14.44 SS.reconfigure SSexhale @23:11:14.48 SS.seek 0ml @23:11:24.01 Exhaust.select :close @23:11:24.14 SSV.dial CBV @23:11:24.16
CBV.dial SSV @23:11:25.04 Allowing 130s for 25.0ml of remaining 675.0ml @23:11:25.05 Intake.select :open @23:11:27.22 Intake @29.8psia, vacuum from 20.0 to 24.0psi @23:11:27.24 SS.reconfigure SSinhale.with maxSpeed: 29 @23:11:27.29 SS.seek 25ml @23:12:28.55 Stroke completed -- waiting for pressure to equalize ... @23:12:31.11 Intake.select :close @23:12:31.27 SSV.dial :exhaust @23:12:32.13 Exhaust.select :open @23:12:32.24 SS.reconfigure SSexhale @23:12:32.29 SS.seek 0ml @23:12:41.76 Exhaust.select :close @23:12:41.87 SSV.dial CBV @23:12:41.90
CBV.dial SSV @23:12:42.79 Allowing 130s for 25.0ml of remaining 650.0ml @23:12:42.80 Intake.select :open @23:12:44.97 Intake @29.7psia, vacuum from 20.0 to 24.0psi @23:12:44.99 SS.reconfigure SSinhale.with maxSpeed: 29 @23:12:45.04 SS.seek 25ml @23:13:46.06 Stroke completed -- waiting for pressure to equalize ... @23:13:48.62 Intake.select :close @23:13:48.77 SSV.dial :exhaust @23:13:49.65 Exhaust.select :open @23:13:49.76 SS.reconfigure SSexhale @23:13:49.82 SS.seek 0ml @23:13:59.31 Exhaust.select :close @23:13:59.43 SSV.dial CBV @23:13:59.46
CBV.dial SSV @23:14:00.34 Allowing 130s for 25.0ml of remaining 625.0ml @23:14:00.35 Intake.select :open @23:14:02.52 Intake @29.8psia, vacuum from 20.0 to 24.0psi @23:14:02.54 SS.reconfigure SSinhale.with maxSpeed: 29 @23:14:02.59 SS.seek 25ml @23:15:03.15 Stroke completed -- waiting for pressure to equalize ... @23:15:06.34 Intake.select :close @23:15:06.49 SSV.dial :exhaust @23:15:07.35 Exhaust.select :open @23:15:07.46 SS.reconfigure SSexhale @23:15:07.51 SS.seek 0ml @23:15:16.81 Exhaust.select :close @23:15:16.93 SSV.dial CBV @23:15:16.95
CBV.dial SSV @23:15:17.85 Allowing 130s for 25.0ml of remaining 600.0ml @23:15:17.86 Intake.select :open @23:15:20.03 Intake @29.7psia, vacuum from 20.0 to 24.0psi @23:15:20.48 SS.reconfigure SSinhale.with maxSpeed: 29 @23:15:20.57 SS.seek 25ml @23:16:21.43 Stroke completed -- waiting for pressure to equalize ... @23:16:24.03 Intake.select :close @23:16:24.19 SSV.dial :exhaust @23:16:25.05 Exhaust.select :open @23:16:25.16 SS.reconfigure SSexhale @23:16:25.21 SS.seek 0ml @23:16:34.70 Exhaust.select :close @23:16:34.82 SSV.dial CBV @23:16:34.85
CBV.dial SSV @23:16:35.74 Allowing 130s for 25.0ml of remaining 575.0ml @23:16:35.75 Intake.select :open @23:16:37.92 Intake @29.7psia, vacuum from 20.0 to 24.0psi @23:16:37.94 SS.reconfigure SSinhale.with maxSpeed: 29 @23:16:37.99 SS.seek 25ml @23:17:38.61 Stroke completed -- waiting for pressure to equalize ... @23:17:41.81 Intake.select :close @23:17:41.97 SSV.dial :exhaust @23:17:42.83 Exhaust.select :open @23:17:42.94 SS.reconfigure SSexhale @23:17:42.99 SS.seek 0ml @23:17:52.34 Exhaust.select :close @23:17:52.46 SSV.dial CBV @23:17:52.49
CBV.dial SSV @23:17:53.37 Allowing 130s for 25.0ml of remaining 550.0ml @23:17:53.38 Intake.select :open @23:17:55.55 Intake @29.6psia, vacuum from 20.0 to 24.0psi @23:17:55.57 SS.reconfigure SSinhale.with maxSpeed: 29 @23:17:55.62 SS.seek 25ml @23:18:56.45 Stroke completed -- waiting for pressure to equalize ... @23:18:59.66 Intake.select :close @23:18:59.81 SSV.dial :exhaust @23:19:00.68 Exhaust.select :open @23:19:00.79 SS.reconfigure SSexhale @23:19:00.84 SS.seek 0ml @23:19:10.53 Exhaust.select :close @23:19:10.65 SSV.dial CBV @23:19:10.68
CBV.dial SSV @23:19:11.57 Allowing 130s for 25.0ml of remaining 525.0ml @23:19:11.58 Intake.select :open @23:19:13.75 Intake @29.7psia, vacuum from 20.0 to 24.0psi @23:19:13.77 SS.reconfigure SSinhale.with maxSpeed: 29 @23:19:13.81 SS.seek 25ml @23:20:14.79 Stroke completed -- waiting for pressure to equalize ... @23:20:17.99 Intake.select :close @23:20:18.15 SSV.dial :exhaust @23:20:19.02 Exhaust.select :open @23:20:19.14 SS.reconfigure SSexhale @23:20:19.18 SS.seek 0ml @23:20:28.62 Exhaust.select :close @23:20:28.74 SSV.dial CBV @23:20:28.77
CBV.dial SSV @23:20:29.66 Allowing 130s for 25.0ml of remaining 500.0ml @23:20:29.67 Intake.select :open @23:20:31.84 Intake @29.7psia, vacuum from 20.0 to 24.0psi @23:20:31.86 SS.reconfigure SSinhale.with maxSpeed: 29 @23:20:31.90 SS.seek 25ml @23:21:02.28 Vacuum too high -- waiting up to 40s @23:21:03.58 Intake @29.0psia, vacuum from 20.0 to 24.0psi @23:21:03.60 SS.reconfigure SSinhale.with maxSpeed: 26 @23:21:03.65 SS.seek 25ml @23:21:37.65 Stroke completed -- waiting for pressure to equalize ... @23:21:40.26 Intake.select :close @23:21:40.42 SSV.dial :exhaust @23:21:41.27 Exhaust.select :open @23:21:41.38 SS.reconfigure SSexhale @23:21:41.43 SS.seek 0ml @23:21:50.73 Exhaust.select :close @23:21:50.85 SSV.dial CBV @23:21:50.88
CBV.dial SSV @23:21:51.76 Allowing 130s for 25.0ml of remaining 475.0ml @23:21:51.77 Intake.select :open @23:21:53.95 Intake @29.8psia, vacuum from 20.0 to 24.0psi @23:21:53.96 SS.reconfigure SSinhale.with maxSpeed: 26 @23:21:54.01 SS.seek 25ml @23:23:01.56 Stroke completed -- waiting for pressure to equalize ... @23:23:04.12 Intake.select :close @23:23:04.28 SSV.dial :exhaust @23:23:05.15 Exhaust.select :open @23:23:05.26 SS.reconfigure SSexhale @23:23:05.32 SS.seek 0ml @23:23:14.68 Exhaust.select :close @23:23:14.80 SSV.dial CBV @23:23:14.83
CBV.dial SSV @23:23:15.72 Allowing 130s for 25.0ml of remaining 450.0ml @23:23:15.73 Intake.select :open @23:23:17.90 Intake @29.6psia, vacuum from 20.0 to 24.0psi @23:23:17.92 SS.reconfigure SSinhale.with maxSpeed: 26 @23:23:17.97 SS.seek 25ml @23:24:25.62 Stroke completed -- waiting for pressure to equalize ... @23:24:28.19 Intake.select :close @23:24:28.34 SSV.dial :exhaust @23:24:29.21 Exhaust.select :open @23:24:29.32 SS.reconfigure SSexhale @23:24:29.38 SS.seek 0ml @23:24:38.80 Exhaust.select :close @23:24:38.91 SSV.dial CBV @23:24:38.94
CBV.dial SSV @23:24:39.82 Allowing 130s for 25.0ml of remaining 425.0ml @23:24:39.83 Intake.select :open @23:24:42.01 Intake @29.5psia, vacuum from 20.0 to 24.0psi @23:24:42.02 SS.reconfigure SSinhale.with maxSpeed: 26 @23:24:42.07 SS.seek 25ml @23:25:49.70 Stroke completed -- waiting for pressure to equalize ... @23:25:52.88 Intake.select :close @23:25:53.02 SSV.dial :exhaust @23:25:53.89 Exhaust.select :open @23:25:54.00 SS.reconfigure SSexhale @23:25:54.05 SS.seek 0ml @23:26:03.46 Exhaust.select :close @23:26:03.57 SSV.dial CBV @23:26:03.60
CBV.dial SSV @23:26:04.50 Allowing 130s for 25.0ml of remaining 400.0ml @23:26:04.51 Intake.select :open @23:26:06.68 Intake @29.5psia, vacuum from 20.0 to 24.0psi @23:26:06.70 SS.reconfigure SSinhale.with maxSpeed: 26 @23:26:06.75 SS.seek 25ml @23:27:14.21 Stroke completed -- waiting for pressure to equalize ... @23:27:17.88 Intake.select :close @23:27:18.04 SSV.dial :exhaust @23:27:18.91 Exhaust.select :open @23:27:19.03 SS.reconfigure SSexhale @23:27:19.07 SS.seek 0ml @23:27:28.58 Exhaust.select :close @23:27:28.69 SSV.dial CBV @23:27:28.72
CBV.dial SSV @23:27:29.63 Allowing 130s for 25.0ml of remaining 375.0ml @23:27:29.64 Intake.select :open @23:27:31.82 Intake @29.2psia, vacuum from 20.0 to 24.0psi @23:27:31.83 SS.reconfigure SSinhale.with maxSpeed: 26 @23:27:31.88 SS.seek 25ml @23:28:39.65 Stroke completed -- waiting for pressure to equalize ... @23:28:42.85 Intake.select :close @23:28:43.01 SSV.dial :exhaust @23:28:43.88 Exhaust.select :open @23:28:43.99 SS.reconfigure SSexhale @23:28:44.04 SS.seek 0ml @23:28:53.53 Exhaust.select :close @23:28:53.64 SSV.dial CBV @23:28:53.67
CBV.dial SSV @23:28:54.57 Allowing 130s for 25.0ml of remaining 350.0ml @23:28:54.58 Intake.select :open @23:28:56.75 Intake @29.4psia, vacuum from 20.0 to 24.0psi @23:28:56.77 SS.reconfigure SSinhale.with maxSpeed: 26 @23:28:56.82 SS.seek 25ml @23:30:04.16 Stroke completed -- waiting for pressure to equalize ... @23:30:07.35 Intake.select :close @23:30:07.51 SSV.dial :exhaust @23:30:08.38 Exhaust.select :open @23:30:08.49 SS.reconfigure SSexhale @23:30:08.54 SS.seek 0ml @23:30:18.32 Exhaust.select :close @23:30:18.44 SSV.dial CBV @23:30:18.47
CBV.dial SSV @23:30:19.35 Allowing 130s for 25.0ml of remaining 325.0ml @23:30:19.36 Intake.select :open @23:30:21.53 Intake @29.4psia, vacuum from 20.0 to 24.0psi @23:30:21.55 SS.reconfigure SSinhale.with maxSpeed: 26 @23:30:21.60 SS.seek 25ml @23:31:29.41 Stroke completed -- waiting for pressure to equalize ... @23:31:31.98 Intake.select :close @23:31:32.14 SSV.dial :exhaust @23:31:33.00 Exhaust.select :open @23:31:33.11 SS.reconfigure SSexhale @23:31:33.16 SS.seek 0ml @23:31:42.69 Exhaust.select :close @23:31:42.81 SSV.dial CBV @23:31:42.84
CBV.dial SSV @23:31:43.72 Allowing 130s for 25.0ml of remaining 300.0ml @23:31:43.73 Intake.select :open @23:31:45.90 Intake @29.4psia, vacuum from 20.0 to 24.0psi @23:31:45.92 SS.reconfigure SSinhale.with maxSpeed: 26 @23:31:45.96 SS.seek 25ml @23:32:53.43 Stroke completed -- waiting for pressure to equalize ... @23:32:56.62 Intake.select :close @23:32:56.78 SSV.dial :exhaust @23:32:57.63 Exhaust.select :open @23:32:57.74 SS.reconfigure SSexhale @23:32:57.80 SS.seek 0ml @23:33:07.62 Exhaust.select :close @23:33:07.74 SSV.dial CBV @23:33:07.77
CBV.dial SSV @23:33:08.66 Allowing 130s for 25.0ml of remaining 275.0ml @23:33:08.67 Intake.select :open @23:33:10.84 Intake @29.3psia, vacuum from 20.0 to 24.0psi @23:33:10.86 SS.reconfigure SSinhale.with maxSpeed: 26 @23:33:10.91 SS.seek 25ml @23:34:18.57 Stroke completed -- waiting for pressure to equalize ... @23:34:21.79 Intake.select :close @23:34:21.95 SSV.dial :exhaust @23:34:22.80 Exhaust.select :open @23:34:22.92 SS.reconfigure SSexhale @23:34:22.96 SS.seek 0ml @23:34:32.33 Exhaust.select :close @23:34:32.46 SSV.dial CBV @23:34:32.48
CBV.dial SSV @23:34:33.36 Allowing 130s for 25.0ml of remaining 250.0ml @23:34:33.37 Intake.select :open @23:34:35.54 Intake @29.3psia, vacuum from 20.0 to 24.0psi @23:34:35.56 SS.reconfigure SSinhale.with maxSpeed: 26 @23:34:35.61 SS.seek 25ml @23:35:43.69 Stroke completed -- waiting for pressure to equalize ... @23:35:48.11 Intake.select :close @23:35:48.27 SSV.dial :exhaust @23:35:49.14 Exhaust.select :open @23:35:49.25 SS.reconfigure SSexhale @23:35:49.31 SS.seek 0ml @23:35:58.69 Exhaust.select :close @23:35:58.81 SSV.dial CBV @23:35:58.84
CBV.dial SSV @23:35:59.73 Allowing 130s for 25.0ml of remaining 225.0ml @23:35:59.74 Intake.select :open @23:36:01.91 Intake @29.3psia, vacuum from 20.0 to 24.0psi @23:36:01.93 SS.reconfigure SSinhale.with maxSpeed: 26 @23:36:01.98 SS.seek 25ml @23:37:09.94 Stroke completed -- waiting for pressure to equalize ... @23:37:13.75 Intake.select :close @23:37:13.90 SSV.dial :exhaust @23:37:14.78 Exhaust.select :open @23:37:14.89 SS.reconfigure SSexhale @23:37:14.96 SS.seek 0ml @23:37:24.33 Exhaust.select :close @23:37:24.45 SSV.dial CBV @23:37:24.48
CBV.dial SSV @23:37:25.36 Allowing 130s for 25.0ml of remaining 200.0ml @23:37:25.37 Intake.select :open @23:37:27.54 Intake @29.1psia, vacuum from 20.0 to 24.0psi @23:37:27.56 SS.reconfigure SSinhale.with maxSpeed: 26 @23:37:27.60 SS.seek 25ml @23:38:35.21 Stroke completed -- waiting for pressure to equalize ... @23:38:39.02 Intake.select :close @23:38:39.18 SSV.dial :exhaust @23:38:40.05 Exhaust.select :open @23:38:40.16 SS.reconfigure SSexhale @23:38:40.21 SS.seek 0ml @23:38:49.71 Exhaust.select :close @23:38:50.31 SSV.dial CBV @23:38:50.34
CBV.dial SSV @23:38:51.23 Allowing 130s for 25.0ml of remaining 175.0ml @23:38:51.24 Intake.select :open @23:38:53.42 Intake @29.3psia, vacuum from 20.0 to 24.0psi @23:38:53.43 SS.reconfigure SSinhale.with maxSpeed: 26 @23:38:53.49 SS.seek 25ml @23:39:48.16 Vacuum too high -- waiting up to 40s @23:39:49.00 Intake @28.0psia, vacuum from 20.0 to 24.0psi @23:39:49.02 SS.reconfigure SSinhale.with maxSpeed: 23 @23:39:49.07 SS.seek 25ml @23:40:04.30 Stroke completed -- waiting for pressure to equalize ... @23:40:07.48 Intake.select :close @23:40:07.64 SSV.dial :exhaust @23:40:08.50 Exhaust.select :open @23:40:08.61 SS.reconfigure SSexhale @23:40:08.67 SS.seek 0ml @23:40:18.07 Exhaust.select :close @23:40:18.19 SSV.dial CBV @23:40:18.22
CBV.dial SSV @23:40:19.11 Allowing 130s for 25.0ml of remaining 150.0ml @23:40:19.12 Intake.select :open @23:40:21.29 Intake @29.1psia, vacuum from 20.0 to 24.0psi @23:40:21.31 SS.reconfigure SSinhale.with maxSpeed: 23 @23:40:21.36 SS.seek 25ml @23:41:37.91 Stroke completed -- waiting for pressure to equalize ... @23:41:41.09 Intake.select :close @23:41:41.24 SSV.dial :exhaust @23:41:42.11 Exhaust.select :open @23:41:42.23 SS.reconfigure SSexhale @23:41:42.27 SS.seek 0ml @23:41:51.76 Exhaust.select :close @23:41:51.87 SSV.dial CBV @23:41:51.90
CBV.dial SSV @23:41:52.80 Allowing 130s for 25.0ml of remaining 125.0ml @23:41:52.81 Intake.select :open @23:41:54.98 Intake @29.0psia, vacuum from 20.0 to 24.0psi @23:41:55.00 SS.reconfigure SSinhale.with maxSpeed: 23 @23:41:55.05 SS.seek 25ml @23:43:11.50 Stroke completed -- waiting for pressure to equalize ... @23:43:14.07 Intake.select :close @23:43:14.22 SSV.dial :exhaust @23:43:15.09 Exhaust.select :open @23:43:15.20 SS.reconfigure SSexhale @23:43:15.26 SS.seek 0ml @23:43:24.65 Exhaust.select :close @23:43:24.76 SSV.dial CBV @23:43:24.79
CBV.dial SSV @23:43:25.69 Allowing 130s for 25.0ml of remaining 100.0ml @23:43:25.70 Intake.select :open @23:43:27.87 Intake @28.9psia, vacuum from 20.0 to 24.0psi @23:43:27.89 SS.reconfigure SSinhale.with maxSpeed: 23 @23:43:27.93 SS.seek 25ml @23:44:44.77 Stroke completed -- waiting for pressure to equalize ... @23:44:47.96 Intake.select :close @23:44:48.10 SSV.dial :exhaust @23:44:48.97 Exhaust.select :open @23:44:49.08 SS.reconfigure SSexhale @23:44:49.13 SS.seek 0ml @23:44:59.20 Exhaust.select :close @23:44:59.32 SSV.dial CBV @23:44:59.34
CBV.dial SSV @23:45:00.23 Allowing 130s for 25.0ml of remaining 75.0ml @23:45:00.24 Intake.select :open @23:45:02.42 Intake @28.9psia, vacuum from 20.0 to 24.0psi @23:45:02.43 SS.reconfigure SSinhale.with maxSpeed: 23 @23:45:02.48 SS.seek 25ml @23:46:18.85 Stroke completed -- waiting for pressure to equalize ... @23:46:22.07 Intake.select :close @23:46:22.23 SSV.dial :exhaust @23:46:23.09 Exhaust.select :open @23:46:23.20 SS.reconfigure SSexhale @23:46:23.25 SS.seek 0ml @23:46:32.66 Exhaust.select :close @23:46:32.78 SSV.dial CBV @23:46:32.81
CBV.dial SSV @23:46:33.68 Allowing 130s for 25.0ml of remaining 50.0ml @23:46:33.69 Intake.select :open @23:46:35.86 Intake @28.9psia, vacuum from 20.0 to 24.0psi @23:46:35.88 SS.reconfigure SSinhale.with maxSpeed: 23 @23:46:35.93 SS.seek 25ml @23:47:52.42 Stroke completed -- waiting for pressure to equalize ... @23:47:55.60 Intake.select :close @23:47:55.75 SSV.dial :exhaust @23:47:56.61 Exhaust.select :open @23:47:56.72 SS.reconfigure SSexhale @23:47:56.77 SS.seek 0ml @23:48:06.44 Exhaust.select :close @23:48:06.56 SSV.dial CBV @23:48:06.59
CBV.dial SSV @23:48:07.46 Allowing 130s for 25.0ml of remaining 25.0ml @23:48:07.47 Intake.select :open @23:48:09.64 Intake @28.9psia, vacuum from 20.0 to 24.0psi @23:48:09.66 SS.reconfigure SSinhale.with maxSpeed: 23 @23:48:09.71 SS.seek 24.95ml @23:49:25.88 Stroke completed -- waiting for pressure to equalize ... @23:49:29.68 Intake.select :close @23:49:29.83 SSV.dial :exhaust @23:49:30.70 Exhaust.select :open @23:49:30.81 SS.reconfigure SSexhale @23:49:30.87 SS.seek 0ml @23:49:40.38 Exhaust.select :close @23:49:40.51 Sampled 1500.0ml @23:49:40.53 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @23:49:40.54 Intake.select :close @23:49:40.60 CSV.dial :waste @23:49:40.68 CS.seek 0ml @23:49:40.78 CSV.dial :air @23:49:40.82 SSV.dial CBV,avoiding: :exhaust @23:49:40.86
CBV.dial SSV @23:49:41.69 CS.seek 10ml @23:49:43.04
CTV.dial :air @23:49:43.52 Gate.power :sampler,:OFF @23:49:43.90
delay 15 seconds @23:49:58.93 SSV.dial :spit,avoiding: :exhaust @23:50:00.42 CTV.dial CSV,avoiding: :intake @23:50:00.47
SS.seek 0ml @23:50:00.59 SSV.dial CBV,avoiding: :exhaust @23:50:08.83 CBV.dial between SSV and waste2 @23:50:09.73 CTV.dial CSV,avoiding: :air @23:50:09.75
CSV.dial CTV @23:50:10.27 CS.reconfigure CSpressurize @23:50:10.31 CS.seek 0ml @23:50:36.20 BubblePoint: Intake @67.2psia, Outlet @11.6psia @23:50:36.21 CBV.dial SSV @23:50:36.57 SS.reconfigure SSconfig @23:50:36.62 SS.seek 10ml @23:50:42.03 delay 15 seconds @23:50:57.17 Evacuated: Intake @34.5psia, Outlet @-2.1psia @23:50:57.18 SSV.dial :spit,avoiding: :exhaust @23:50:58.68 SS.reconfigure SSexhale @23:50:58.73 SS.seek 0ml @23:51:03.85 SS.reconfigure SSfast @23:51:03.90 SS.seek 5ml @23:51:06.81 SSV.dial CBV,avoiding: :exhaust @23:51:06.84
CBV.dial SSV @23:51:08.35 CTV.dial :air,avoiding: :intake @23:51:09.87 CSV.dial CTV @23:51:09.92
SS.seek 10ml @23:51:10.92 CSV.dial :air @23:51:11.43 CS.reconfigure CSfast @23:51:11.48 CS.seek 0ml @23:51:12.48
SSV.dial :spit,avoiding: :exhaust @23:51:12.70 CS.coast @23:51:13.97
SS.reconfigure SSexhale @23:51:14.02 SS.seek 0ml @23:51:19.15 SS.reconfigure SSfast @23:51:19.21 SS.seek 5ml @23:51:22.17 SSV.dial CBV,avoiding: :exhaust @23:51:22.20
CBV.dial SSV @23:51:24.72 SSV.dial :spit,avoiding: :exhaust @23:51:26.23 SS.reconfigure SSconfig @23:51:26.28 SS.seek 0ml @23:51:29.59 SS.coast @23:51:29.64 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @23:51:29.65 Intake.select :close @23:51:29.73 CSV.dial :waste @23:51:29.88 SSV.dial CBV,avoiding: :exhaust @23:51:29.90
CBV.dial SSV @23:51:30.64 CS.seek 0ml @23:51:30.71 CSV.dial :air @23:51:31.40
CTV.dial :air @23:51:31.42 delay 15 seconds @23:51:31.53 CS.seek 10ml @23:51:46.44
SSV.dial :spit,avoiding: :exhaust @23:51:47.93 CTV.dial CSV,avoiding: :intake @23:51:47.97
SS.reconfigure SSexhale @23:51:48.08 SS.seek 0ml @23:51:48.14 SSV.dial CBV,avoiding: :exhaust @23:51:58.05 CBV.dial between SSV and waste2 @23:51:58.48 CTV.dial CSV,avoiding: :air @23:51:58.51
CSV.dial CTV @23:51:59.02 CS.reconfigure CSpressurize @23:51:59.07 CS.seek 0ml @23:52:25.21 CSV.dial :air @23:52:25.71 CS.reconfigure CSfast @23:52:25.76 CS.seek 10ml @23:52:52.05 CSV.dial between air and CTV @23:52:52.42 CS.seek 3.207ml @23:53:11.18 CTV.dial CSV,avoiding: :air @23:53:11.21
CSV.dial CTV @23:53:11.55 CS.reconfigure CSpressurize @23:53:11.59 CS.seek 0ml @23:53:19.58 BubblePoint: Intake @66.4psia, Outlet @11.1psia @23:53:19.59 CBV.dial SSV @23:53:19.94 SS.reconfigure SSconfig @23:53:19.99 SS.seek 10ml @23:53:25.57 delay 15 seconds @23:53:40.71 Evacuated: Intake @41.3psia, Outlet @10.7psia @23:53:40.72 SSV.dial :spit,avoiding: :exhaust @23:53:42.21 SS.reconfigure SSexhale @23:53:42.26 SS.seek 0ml @23:53:47.85 SS.reconfigure SSfast @23:53:47.90 SS.seek 5ml @23:53:50.65 SSV.dial CBV,avoiding: :exhaust @23:53:50.68
CBV.dial SSV @23:53:52.18 CTV.dial :air,avoiding: :intake @23:53:53.68 CSV.dial CTV @23:53:53.72
SS.seek 10ml @23:53:54.72 CSV.dial :air @23:53:55.23 CS.reconfigure CSfast @23:53:55.29 CS.seek 0ml @23:53:56.46
SSV.dial :spit,avoiding: :exhaust @23:53:57.23 CS.coast @23:53:57.95
SS.reconfigure SSexhale @23:53:58.00 SS.seek 0ml @23:54:03.52 SS.reconfigure SSfast @23:54:03.57 SS.seek 5ml @23:54:06.48 SSV.dial CBV,avoiding: :exhaust @23:54:06.51
CBV.dial SSV @23:54:09.03 SSV.dial :spit,avoiding: :exhaust @23:54:10.51 SS.reconfigure SSconfig @23:54:10.56 SS.seek 0ml @23:54:13.68 SS.coast @23:54:13.72 Lysing: Adding 1.5ml lysis @23:54:13.73 CBV.dial between CSV and waste1,avoiding: :DBV @23:54:15.40 CTV.dial CSV @23:54:16.88 CSV.dial CSR @23:54:17.76 Pulling 1ml of CSR.lysis @23:54:17.77 CSR.select :lysis @23:54:17.89 CS.reconfigure CSconfig @23:54:17.94 CS.seek 1ml @23:54:21.56 delay 5 seconds @23:54:26.59 CSR.select :air @23:54:26.77 CS.seek 6ml @23:54:40.49 delay 2 seconds @23:54:42.51 CSV.dial :waste1 @23:54:43.96 CS.reconfigure CSfast @23:54:44.01 CS.seek :empty @23:55:00.34 CSV.dial CSR @23:55:01.87 Pulling 1.5ml of CSR.lysis @23:55:01.88 CSR.select :lysis @23:55:02.00 CS.reconfigure CSconfig @23:55:02.05 CS.seek 1.5ml @23:55:06.90 delay 5 seconds @23:55:11.93 CSR.select :air @23:55:12.10 CS.seek 4.5ml @23:55:20.77 delay 2 seconds @23:55:22.79 CSV.dial CTV @23:55:23.30 CS.seek 1.275ml @23:55:32.60 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @23:55:32.62 CH.seek 85C,8:00 @23:58:56.45 CH.hold 5:00,85C @00:03:56.62CEST08-Sep-18 Collection Puck Heater at 84.9C after lysing @00:03:56.64 CS.seek 0ml @00:04:00.84 CTV.dial between spare and air,avoiding: :intake @00:04:02.01 CSV.dial :air @00:04:02.51 CS.seek 5ml @00:04:16.30 CSV.dial CTV @00:04:16.81 delay 2 seconds @00:04:18.83 CSV.dial :air @00:04:19.34 delay 2 seconds @00:04:21.36 CSV.dial CTV @00:04:21.87 delay 2 seconds @00:04:23.89 CSV.dial :air @00:04:24.40 delay 2 seconds @00:04:26.42 CS.seek 0ml @00:04:40.44 Recovering Lysate into Collection syringe @00:04:40.45 CSV.dial CBV @00:04:41.70 CBV.dial CSV @00:04:42.11 CS.seek 5ml @00:04:55.90 delay 5 seconds @00:05:00.92 CTV.dial :air @00:05:01.49 CS.seek 7ml @00:05:08.12 delay 5 seconds @00:05:13.14 CSV.dial :air2 @00:05:13.66 CS.seek 10ml @00:05:22.40 CSV.dial between air and CTV,avoiding: CBV @00:05:23.69 Lysing Complete @00:05:23.77 Storing sh1 puck in Collection Clamp to tube 1 @00:05:24.48 SC.reconfigure SCtight @00:05:24.53 SC.seek 1 @00:05:26.65 SC.reconfigure SCloose @00:05:26.88 Elbow.reconfigure TightElbow @00:05:26.93 Elbow.seek 1 @00:05:28.86 Forearm.reconfigure TightForearm @00:05:28.91 Forearm.seek 1 + 700 counts @00:05:30.00 SE.reconfigure SEpick @00:05:30.09 SE.seek beamBreak @00:05:33.02 SE.configure SEconfig @00:05:33.07 SE.seek :place @00:05:33.79 Forearm.reconfigure LooseForearm @00:05:33.84 Forearm.seek :retract @00:05:34.76 Elbow.seek CC @00:05:36.36 Elbow.reconfigure LooseElbow @00:05:36.47 Forearm.seek CC @00:05:37.45 Hand.close @00:05:38.16 CC.seek :open @00:05:42.69 Forearm.seek :retract @00:05:43.69 Elbow.reconfigure TightElbow @00:05:43.74 Elbow.seek 1 @00:05:45.67 Elbow.reconfigure LimpElbow @00:05:45.72 Forearm.seek 1 @00:05:46.79 Elbow.hold @00:05:46.86 Elbow.reconfigure TightElbow @00:05:46.91 Hand.open @00:05:47.80 Forearm.force -20 @00:05:47.84 Forearm.seek 1 + 336 counts @00:05:48.17 Forearm.reconfigure TightForearm @00:05:48.22 Forearm.seek 1 + 700 counts @00:05:48.62 SE.seek :down @00:05:49.77
Forearm.reconfigure LooseForearm @00:05:49.82 Forearm.seek :retract @00:05:51.45 SE.coast @00:05:51.50
SC.coast @00:05:51.54 Tranferring lysate to PRV @00:05:51.55 CSV.dial :air @00:05:51.88 CS.seek 10ml @00:05:51.97 CSV.dial between air and CTV @00:05:52.33 AIV.dial :bypass @00:05:52.42 Beginning samplePassC @00:05:52.43 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @00:05:52.51 Hand.open @00:05:52.94 Elbow.seek FlushPuck::Garage @00:05:54.90 Elbow.reconfigure LooseElbow @00:05:55.51 Forearm.seek :garage @00:05:56.52 Hand.close @00:05:57.42 Forearm.seek :retract @00:05:58.46 Elbow.reconfigure TightElbow @00:05:58.51 Elbow.seek CC @00:06:01.36 Elbow.reconfigure LooseElbow @00:06:01.47 Forearm.reconfigure TightForearm @00:06:01.52 Forearm.seek CC @00:06:02.83 CC.seek :closed @00:06:07.09 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.37 @00:06:07.16 Hand.open @00:06:07.98 Forearm.reconfigure LooseForearm @00:06:08.02 Forearm.seek :retract @00:06:09.02 AWV.select :air @00:06:09.03 AIV.dial :bypass @00:06:09.04 PSV.dial PSR @00:06:10.57 Pulling 1.5ml of PSR.flush @00:06:10.58 PSR.select :flush @00:06:10.71 PS.seek 1.5ml @00:06:26.96 delay 5 seconds @00:06:31.99 PSV.dial :waste1 @00:06:32.50 PS.seek 0ml @00:06:48.73 PSV.dial PSR @00:06:49.24 PSR.select :air @00:06:49.36 PS.seek 2.5ml @00:07:16.05 PRV.dial CBV @00:07:16.10
CBV.dial PRV @00:07:16.98 PSV.dial :mixloop @00:07:18.13 CTV.dial :air,avoiding: :intake @00:07:18.14 PTV.dial :PRVmixing @00:07:18.97 PS.seek 0ml @00:07:45.58 PSV.dial :air @00:07:46.10 PS.seek 2.5ml @00:08:12.64 PSV.dial :mixloop @00:08:13.17 PS.seek 0ml @00:08:39.83 PSV.dial :air @00:08:40.35 PS.seek 2.5ml @00:09:06.65 PSV.dial :mixloop @00:09:07.16 PS.seek 0ml @00:09:33.74 Garaging FlushPuck in Collection Clamp @00:09:33.82 Elbow.reconfigure TightElbow @00:09:33.87 Elbow.seek CC @00:09:34.12 Elbow.reconfigure LooseElbow @00:09:34.23 Forearm.seek CC @00:09:35.24 Hand.close @00:09:36.04 CC.seek :open @00:09:40.41 Forearm.seek :retract @00:09:41.39 Elbow.reconfigure TightElbow @00:09:41.44 Elbow.seek :garage @00:09:44.56 Elbow.reconfigure LooseElbow @00:09:44.66 Forearm.reconfigure TightForearm @00:09:44.71 Forearm.seek :garage @00:09:46.36 Hand.open @00:09:47.24 Forearm.reconfigure LooseForearm @00:09:47.29 Forearm.seek :retract @00:09:48.31 Connecting PS to CS to transfer sample @00:09:48.32 CBV.dial AIV @00:09:48.33 AIV.dial :bypass @00:09:48.34 PSV.dial :mixloop @00:09:48.36 PTV.dial :PRVmixing @00:09:48.37 PRV.dial AIV @00:09:48.38 CTV.dial :air,avoiding: :intake @00:09:48.40 Loading lyfil puck from tube 2 into Collection Clamp @00:09:48.66 SC.reconfigure SCtight @00:09:48.70 SC.seek 2 @00:09:51.10 SC.reconfigure SCloose @00:09:51.32 Elbow.reconfigure TightElbow @00:09:51.37 Elbow.seek 2 @00:09:53.45 Forearm.reconfigure TightForearm @00:09:53.50 Forearm.seek 2 + 700 counts @00:09:54.54 SE.reconfigure SEpick @00:09:54.62 SE.seek beamBreak @00:09:57.65 SE.configure SEconfig @00:09:57.83 Elbow.seek 2 @00:09:58.03 Forearm.seek 2 + 300 counts @00:09:58.26 Forearm.reconfigure LooseForearm @00:09:58.30 Hand.close @00:09:59.06 SE.seek :down @00:09:59.28
Forearm.seek :retract @00:10:00.23 Elbow.seek CC @00:10:01.81 Elbow.reconfigure LooseElbow @00:10:01.93 SE.coast @00:10:01.96
Forearm.reconfigure TightForearm @00:10:02.04 Forearm.seek CC @00:10:03.35 CC.seek :closed @00:10:07.62 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.42 @00:10:07.63 Hand.open @00:10:08.52 Forearm.reconfigure LooseForearm @00:10:08.57 Forearm.seek :retract @00:10:09.56 SC.coast @00:10:09.59 CTV.dial CSV,avoiding: :intake @00:10:11.10 CSV.dial CTV @00:10:11.43 CS.seek 5ml @00:10:25.41 delay 1:20 @00:11:45.45 CS.seek 2.5ml @00:11:52.81 delay 40 seconds @00:12:32.84 CS.seek 0ml @00:12:40.44 delay 1:00 @00:13:40.47 PSV.dial :air @00:13:40.99 PS.seek 2.5ml @00:14:07.36 PSV.dial :mixloop @00:14:07.85 delay 3 seconds @00:14:10.87 PSV.dial :air @00:14:11.35 delay 2 seconds @00:14:13.37 PSV.dial :mixloop @00:14:13.85 delay 3 seconds @00:14:16.88 PSV.dial :waste1 @00:14:18.35 PS.seek :empty @00:14:44.98 CBV.dial :waste1 @00:14:46.02 delay 5 seconds @00:14:51.05 CBV.dial AIV @00:14:51.57 delay 10 seconds @00:15:01.60 CBV.dial :waste1 @00:15:02.13 delay 10 seconds @00:15:12.16 CBV.dial AIV @00:15:12.68 delay 10 seconds @00:15:22.71 CTV.dial CSV,avoiding: :intake @00:15:22.72 CBV.dial between AIV and waste1 @00:15:23.10 CSV.dial :air @00:15:23.62 CS.seek 10ml @00:15:50.34 CSV.dial CTV @00:15:50.86 CS.seek 0ml @00:16:17.49 CBV.dial :waste1 @00:16:17.83 delay 10 seconds @00:16:27.86 CTV.dial :air,avoiding: :intake @00:16:29.42 Storing lyfil puck in Collection Clamp to tube 1 @00:16:29.60 SC.reconfigure SCtight @00:16:29.65 SC.seek 1 @00:16:31.79 SC.reconfigure SCloose @00:16:32.02 Elbow.reconfigure TightElbow @00:16:32.07 Elbow.seek 1 @00:16:33.96 Forearm.reconfigure TightForearm @00:16:34.01 Forearm.seek 1 + 700 counts @00:16:35.09 SE.reconfigure SEpick @00:16:35.17 SE.seek beamBreak @00:16:38.30 SE.configure SEconfig @00:16:38.47 Elbow.reconfigure LimpElbow @00:16:38.52 Forearm.reconfigure LooseForearm @00:16:38.58 Forearm.seek 1 @00:16:38.87 Elbow.hold @00:16:38.94 Elbow.reconfigure TightElbow @00:16:39.04 SE.seek 0.06 Pucks below Top Plate @00:16:39.51 Forearm.force -20 @00:16:39.55 Forearm.seek 1 + 350 counts @00:16:39.89 Forearm.reconfigure TightForearm @00:16:39.94 Forearm.seek 1 + 700 counts @00:16:40.17 SE.seek 1.06 Pucks below Top Plate @00:16:40.76 Forearm.reconfigure LooseForearm @00:16:40.80 Forearm.seek :retract @00:16:41.71 Elbow.seek CC @00:16:43.30 Elbow.reconfigure LooseElbow @00:16:43.40 Forearm.seek CC @00:16:44.40 Hand.close @00:16:45.18 CC.seek :open @00:16:49.70 Forearm.seek :retract @00:16:50.66 Elbow.reconfigure TightElbow @00:16:50.71 Elbow.seek 1 @00:16:52.55 Elbow.reconfigure LimpElbow @00:16:52.61 Forearm.seek 1 @00:16:53.66 Elbow.hold @00:16:53.73 Elbow.reconfigure TightElbow @00:16:53.78 Hand.open @00:16:54.69 Forearm.force -20 @00:16:54.73 Forearm.seek 1 + 349 counts @00:16:55.57 Forearm.reconfigure TightForearm @00:16:55.62 Forearm.seek 1 + 700 counts @00:16:55.91 SE.seek :down @00:16:57.06
Forearm.reconfigure LooseForearm @00:16:57.11 Forearm.seek :retract @00:16:58.63 SE.coast @00:16:58.67
SC.coast @00:16:58.71 PSV.dial :air @00:16:59.96 PS.seek 0.499ml @00:17:05.90 delay 3 seconds @00:17:08.92 PSV.dial :mixloop @00:17:09.40 PRV.dial :air @00:17:09.88 delay 3 seconds @00:17:12.91 PRV.dial CBV @00:17:13.42 CBV.dial AIV @00:17:13.94 PRV.dial between CBV and air @00:17:14.29 PRV.dial :air @00:17:14.69 PS.reconfigure PSslow @00:17:14.74 PS.seek 0.599ml @00:17:17.37 PRV.dial :waste2 @00:17:18.18 PS.reconfigure PSconfig @00:17:18.23 PS.seek :empty @00:17:25.18 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @00:17:25.25 Hand.open @00:17:25.68 Elbow.seek FlushPuck::Garage @00:17:27.60 Elbow.reconfigure LooseElbow @00:17:27.70 Forearm.seek :garage @00:17:28.72 Hand.close @00:17:29.56 Forearm.seek :retract @00:17:30.59 Elbow.reconfigure TightElbow @00:17:30.64 Elbow.seek CC @00:17:33.44 Elbow.reconfigure LooseElbow @00:17:33.54 Forearm.reconfigure TightForearm @00:17:33.59 Forearm.seek CC @00:17:34.89 CC.seek :closed @00:17:39.16 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.32 @00:17:39.23 Hand.open @00:17:40.00 Forearm.reconfigure LooseForearm @00:17:40.04 Forearm.seek :retract @00:17:41.10 Clearing PML for modified lysate @00:17:41.12 CTV.dial :air,avoiding: :intake @00:17:41.13 PRV.dial :waste2 @00:17:41.14 PTV.dial :PRV,avoiding: :puck @00:17:41.96 delay 2 seconds @00:17:43.98 PTV.dial :PRVmixing,avoiding: :puck @00:17:44.81 delay 2 seconds @00:17:46.83 PSV.dial :PML @00:17:46.85 delay 2 seconds @00:17:48.87 PRV.dial :air @00:17:49.70 delay 2 seconds @00:17:51.78 PS.seek 1.499ml @00:18:07.81 delay 5 seconds @00:18:12.84 PS.seek 2.5ml @00:18:24.52 PSV.dial :air @00:18:25.05 PS.seek 0ml @00:18:51.62 mixing lysate with speDil @00:18:51.63 PSV.dial :PML @00:18:52.13 CBV.dial :PRV @00:18:52.14 PRV.dial :air @00:18:52.16 delay 2 seconds @00:18:54.18 PRV.dial :speDil @00:18:54.68 delay 2 seconds @00:18:56.77 PS.reconfigure PSslow1 @00:18:56.82 PS.seek 0.225ml @00:19:20.99 delay 5 seconds @00:19:26.02 PRV.dial CBV @00:19:26.87 delay 2 seconds @00:19:28.95 PS.seek 0.475ml @00:19:54.74 delay 5 seconds @00:19:59.77 PRV.dial :air @00:20:00.29 delay 2 seconds @00:20:02.32 PS.seek 0.965ml @00:20:52.87 delay 10 seconds @00:21:02.90 PRV.dial CBV @00:21:03.40 CTV.dial :air,avoiding: :intake @00:21:03.41 PSV.dial :air @00:21:03.92 PS.reconfigure PSconfig @00:21:03.97 PS.seek 2.5ml @00:21:20.37 delay 3 seconds @00:21:23.39 PSV.dial PTV @00:21:24.23 PTV.dial :PRV,avoiding: :puck @00:21:25.07 PS.seek 0ml @00:21:51.20 PSV.dial :air @00:21:52.04 PS.seek 2.5ml @00:22:18.40 delay 3 seconds @00:22:21.42 PSV.dial PTV @00:22:22.26 PTV.dial :PRV,avoiding: :puck @00:22:22.28 PS.seek 0ml @00:22:48.68 Pushing modified lysate into AIV Injection Loop @00:22:48.69 CBV.dial AIV @00:22:48.70 AIV.dial :bypass @00:22:48.71 PRV.dial CBV @00:22:48.73 PSV.dial :air @00:22:49.57 PS.seek 2.5ml @00:23:15.93 delay 3 seconds @00:23:18.95 PSV.dial :PML @00:23:19.45 PTV.dial :PRVmixing,avoiding: :puck @00:23:20.36 PS.reconfigure PSslow1 @00:23:20.41 PS.seek 1.74ml @00:24:37.84 delay 5 seconds @00:24:42.87 AIV.dial :sample @00:24:43.49 AWV.select :waste @00:24:43.66 PS.reconfigure PSslow2 @00:24:43.71 PS.seek 1.22ml @00:26:31.53 delay 15 seconds @00:26:46.56 AIV.dial :bypass @00:26:47.18 PS.reconfigure PSconfig @00:26:47.23 PS.seek 0ml @00:27:00.49 PSV.dial :air @00:27:01.02 PS.seek 2.5ml @00:27:27.17 PSV.dial :PML @00:27:27.69 PS.seek 0ml @00:27:53.84 AIV.dial :coilToMFB @00:27:53.85 AWV.select :air @00:27:54.02 Extracting Nucleic Acid from AIV @00:27:54.05
Beginning samplePassD @00:27:54.08 SPE.hold 2:46:40,55C @00:27:54.17 ASV.dial :SPEdelivery @00:27:54.25
AWV.select :air @00:27:54.26 AIV.dial :bypass @00:27:54.27 PSV.dial PSR @00:27:54.58 delay 20 seconds @00:27:55.54
Pulling 1.5ml of PSR.killMFB @00:27:55.55 PSR.select :killMFB @00:27:55.68 PS.seek 1.5ml @00:28:12.68 delay 5 seconds @00:28:14.61 primeLysateINT @00:28:14.62 AWV.select :air @00:28:14.63 AIV.dial :coilToMFB @00:28:14.65 ATV.dial AIV @00:28:16.52 AS.reconfigure ASslow1 @00:28:16.57 AS.seek 0.299ml @00:28:17.70
PSV.dial :waste1 @00:28:18.23 PS.seek 0ml @00:28:35.31 PSV.dial PSR @00:28:35.97 PSR.select :air @00:28:36.10 PS.seek 2.5ml @00:29:03.81 PRV.dial CBV @00:29:03.84
CBV.dial PRV @00:29:03.85 PSV.dial :mixloop @00:29:05.20 CTV.dial :air,avoiding: :intake @00:29:05.21 PTV.dial :PRVmixing @00:29:05.23 PS.seek 0ml @00:29:17.67 delay 10 seconds @00:29:27.70 ATV.dial :air2 @00:29:28.58 AS.seek 0.329ml @00:29:32.95
PSV.dial PSR @00:29:34.20 Pulling 1.5ml of PSR.flush @00:29:34.21 PSR.select :flush @00:29:34.34 PS.seek 1.5ml @00:29:34.88 ATV.dial :waste2 @00:29:35.47 AS.reconfigure ASnorm @00:29:35.52 AS.seek :empty @00:29:51.44
delay 5 seconds @00:29:52.48 Lysate primed! @00:29:52.49 AFV.select :holdingCoil @00:29:52.50 ATV.dial :air2 @00:29:53.16 AS.reconfigure ASslow1 @00:29:53.21 AS.seek 0.069ml @00:29:56.47
PSV.dial :waste1 @00:29:57.01 PS.seek 0ml @00:30:07.61 delay 3 seconds @00:30:10.64 ATV.dial :AIV @00:30:11.51 AS.seek 0.469ml @00:30:14.12
PSV.dial PSR @00:30:14.76 PSR.select :air @00:30:14.89 PS.seek 2.5ml @00:30:42.28 PRV.dial CBV @00:30:42.31
CBV.dial PRV @00:30:42.32 PSV.dial :mixloop @00:30:43.53 CTV.dial :air,avoiding: :intake @00:30:43.54 PTV.dial :PRVmixing @00:30:43.56 PS.seek 0ml @00:31:11.03 PSV.dial PSR @00:31:12.29 Pulling 1.5ml of PSR.flush @00:31:12.30 PSR.select :flush @00:31:12.43 PS.seek 1.5ml @00:31:29.15 delay 5 seconds @00:31:32.23 delay 5 seconds @00:31:34.18
PSV.dial :waste1 @00:31:34.71 PS.seek 0ml @00:31:37.30 Solid Phase Extraction Heater at 50.6C @00:31:37.31 ATV.dial ASV @00:31:37.72 ASV.dial :SPEdelivery @00:31:37.80 AS.seek 0.041ml @00:31:51.31
PSV.dial PSR @00:31:51.83 PSR.select :air @00:31:51.95 PS.seek 2.5ml @00:32:18.90 PRV.dial CBV @00:32:18.93
CBV.dial PRV @00:32:18.94 PSV.dial :mixloop @00:32:20.11 CTV.dial :air,avoiding: :intake @00:32:20.12 PTV.dial :PRVmixing @00:32:20.14 PS.seek 0ml @00:32:47.06 PSV.dial PSR @00:32:48.28 Pulling 1.5ml of PSR.flush @00:32:48.29 PSR.select :flush @00:32:48.42 PS.seek 1.5ml @00:33:04.17 delay 10 seconds @00:33:04.81
delay 5 seconds @00:33:09.84 PSV.dial :waste1 @00:33:10.36 PS.seek 0ml @00:33:14.19 rinseLysateLineINT @00:33:14.20 AFV.select :carrierSolution @00:33:14.31 delay 2 seconds @00:33:16.34 AS.reconfigure ASconfig @00:33:16.39 AS.seek 1ml @00:33:26.72
PSV.dial PSR @00:33:27.23 PSR.select :air @00:33:27.35 PS.seek 2.5ml @00:33:54.47 PRV.dial CBV @00:33:54.50
CBV.dial PRV @00:33:54.51 PSV.dial :mixloop @00:33:55.69 CTV.dial :air,avoiding: :intake @00:33:55.70 PTV.dial :PRVmixing @00:33:55.72 PS.reconfigure PSslow @00:33:55.77 PS.seek 1.2ml @00:34:11.80 delay 1 second @00:34:12.82 AFV.select :holdingCoil @00:34:12.92 delay 1 second @00:34:13.95 ATV.dial AIV @00:34:14.50 AIV.dial :coilToMFB @00:34:14.51 AWV.select :waste @00:34:14.62 AS.reconfigure ASslow0 @00:34:14.67 AS.seek 0ml @00:34:22.62
PSV.dial :waste1 @00:34:24.11 PS.reconfigure PSconfig @00:34:24.17 PS.seek 0ml @00:34:37.33 PSV.dial :air @00:34:38.51 PS.seek 2.5ml @00:35:05.00 PSV.dial :mixloop @00:35:05.50 PS.reconfigure PSslow @00:35:05.55 PS.seek 0ml @00:35:55.56 ATV.dial :air2 @00:35:56.24 AS.reconfigure ASslow1 @00:35:56.29 AS.seek 0.05ml @00:35:56.69
PSV.dial :air @00:35:57.20 PS.reconfigure PSconfig @00:35:57.25 PS.seek 2.5ml @00:36:06.69 ATV.dial :AIV @00:36:07.63 AS.seek 0.01ml @00:36:15.97 ATV.dial :waste @00:36:17.61 AS.seek :empty @00:36:20.05 ethanol wash @00:36:20.06 ATV.dial :air2 @00:36:21.34 AS.reconfigure ASslow2 @00:36:21.39 AS.seek 0.009ml @00:36:23.87
PSV.dial :mixloop @00:36:24.37 PS.reconfigure PSslow @00:36:24.42 PS.seek 0ml @00:36:32.11 delay 3 seconds @00:36:35.14 Pulling 0.4ml of Ethanol Wash @00:36:35.15 ATV.dial :ethanol @00:36:35.99 AS.reconfigure ASslow1 @00:36:36.04 AS.seek 0.409ml @00:37:15.56
Cleaning Collection Syringe @00:37:15.57 CSV.dial CSR @00:37:16.12 Pulling 4ml of CSR.flush @00:37:16.13 CSR.select :flush @00:37:16.26 CS.seek 4ml @00:37:27.48 delay 5 seconds @00:37:32.51 CSR.select :air @00:37:32.64 CS.seek 10ml @00:37:49.11 CSV.dial CTV @00:37:49.14
CTV.dial CSV @00:37:50.70 CBV.dial :waste1 @00:37:51.23 CS.seek 0ml @00:37:56.75 delay 3 seconds @00:37:59.78 ATV.dial :air1 @00:38:00.17 delay 3 seconds @00:38:03.26 AS.seek 0.434ml @00:38:08.62 delay 3 seconds @00:38:11.65 ATV.dial :ethanol @00:38:12.27 AS.seek 0.419ml @00:38:15.55 delay 3 seconds @00:38:17.79
CSV.dial CSR @00:38:18.37 Pulling 4ml of CSR.flush @00:38:18.38 CSR.select :flush @00:38:18.50 CS.seek 4ml @00:38:18.57 ATV.dial ASV @00:38:19.91 AS.seek 0.092ml @00:38:29.70
delay 5 seconds @00:38:34.73 CSR.select :air @00:38:34.86 CS.seek 10ml @00:38:51.20 CSV.dial CTV @00:38:51.23
CTV.dial CSV @00:38:51.75 CBV.dial :waste1 @00:38:51.77 CS.seek 0ml @00:39:18.83 Garaging FlushPuck in Collection Clamp @00:39:18.91 Elbow.reconfigure TightElbow @00:39:18.96 Elbow.seek CC @00:39:19.21 Elbow.reconfigure LooseElbow @00:39:19.32 Forearm.seek CC @00:39:20.29 Hand.close @00:39:21.08 CC.seek :open @00:39:25.52 Forearm.seek :retract @00:39:25.95 delay 3 seconds @00:39:26.50
Elbow.reconfigure TightElbow @00:39:26.55 Elbow.seek :garage @00:39:28.97 ATV.dial :waste2 @00:39:29.80
Elbow.reconfigure LooseElbow @00:39:29.82 AS.reconfigure ASconfig @00:39:29.94 AS.seek :empty @00:39:30.06
Forearm.reconfigure TightForearm @00:39:30.11 Forearm.seek :garage @00:39:31.28 Hand.open @00:39:32.15 Forearm.reconfigure LooseForearm @00:39:32.20 Forearm.seek :retract @00:39:33.25 Whole Cell Archive sampling 1500ml @00:39:33.27 Loading wcr puck from tube 2 into Collection Clamp @00:39:33.53 SC.reconfigure SCtight @00:39:33.58 SC.seek 2 @00:39:35.64 ATV.dial :air2 @00:39:35.77 SC.reconfigure SCloose @00:39:36.00 Elbow.reconfigure TightElbow @00:39:36.08 Elbow.seek 2 @00:39:36.12 AS.reconfigure ASslow1 @00:39:36.21 AS.seek 1ml @00:39:38.29 Forearm.reconfigure TightForearm @00:39:38.34 Forearm.seek 2 + 700 counts @00:39:39.40 SE.reconfigure SEpick @00:39:39.48 SE.seek beamBreak @00:39:42.53 SE.configure SEconfig @00:39:42.71 Elbow.seek 2 @00:39:43.08 Forearm.seek 2 + 300 counts @00:39:43.27 Forearm.reconfigure LooseForearm @00:39:43.31 Hand.close @00:39:44.10 SE.seek :down @00:39:44.34 Forearm.seek :retract @00:39:45.33 Elbow.seek CC @00:39:46.97 Elbow.reconfigure LooseElbow @00:39:47.07 SE.coast @00:39:47.17 Forearm.reconfigure TightForearm @00:39:47.22 Forearm.seek CC @00:39:48.59 CC.seek :closed @00:39:52.86 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.41 @00:39:52.88 Hand.open @00:39:53.66 Forearm.reconfigure LooseForearm @00:39:53.71 Forearm.seek :retract @00:39:54.73 SC.coast @00:39:54.77 collectSample 1500ml w/bubble point of 55psi @00:39:54.79 Priming 7ml @00:39:54.80 Gate.power :sampler,:ON @00:39:54.81 Intake.select :close @00:39:54.82 Exhaust.select :close @00:39:54.84 SS.hold @00:39:55.06 Calibrating pressure sensors @00:39:55.08 CTV.dial :air @00:39:56.61 SSV.dial :spit,avoiding: :exhaust @00:39:56.63 SS.reconfigure SSfast @00:39:56.68 SS.seek 5ml @00:39:59.70 SSV.dial CBV @00:39:59.73 CBV.dial SSV @00:40:01.33 delay 3.8 seconds @00:40:05.27 SSV.dial :spit @00:40:06.78 SS.seek 0ml @00:40:09.66 SSV.dial CBV @00:40:11.16 CTV.dial :intake @00:40:12.02 Waiting for external sampler to finish priming... @00:40:12.03 delayUntil "00:44:54.81" @00:42:57.80 delay 5 seconds @00:43:02.83 ATV.dial ASV @00:43:03.83 ASV.dial :SPEdelivery @00:43:03.85 AS.seek 0.02ml @00:44:54.83 SSV.dial CBV @00:44:54.86 CBV.dial SSV @00:44:54.88 Allowing 40s for 7.0ml of remaining 7.0ml @00:44:54.89 Intake.select :open @00:44:57.07 Intake @29.6psia, vacuum from 20.0 to 24.0psi @00:44:57.09 SS.reconfigure SSinhale.with maxSpeed: 150 @00:44:57.13 SS.seek 7ml @00:45:02.62 Stroke completed -- waiting for pressure to equalize ... @00:45:12.11 Intake.select :close @00:45:12.27 SSV.dial :exhaust @00:45:13.13 Exhaust.select :open @00:45:13.24 SS.reconfigure SSexhale @00:45:13.29 SS.seek 0ml @00:45:17.33 Exhaust.select :close @00:45:17.45 Sampling 1500.0ml @00:45:17.48 Intake.select :close @00:45:17.49 Exhaust.select :close @00:45:17.50 SS.reconfigure SSconfig @00:45:17.56 SS.hold @00:45:17.78 CTV.dial :intake @00:45:17.80 SSV.dial CBV @00:45:17.85 CBV.dial SSV @00:45:18.72 Allowing 130s for 25.0ml of remaining 1500.0ml @00:45:18.73 Intake.select :open @00:45:20.90 Intake @30.0psia, vacuum from 20.0 to 24.0psi @00:45:20.92 SS.reconfigure SSinhale.with maxSpeed: 150 @00:45:20.97 SS.seek 25ml @00:45:25.13 Vacuum too high -- waiting up to 40s @00:45:26.52 Intake @27.1psia, vacuum from 20.0 to 24.0psi @00:45:26.54 SS.reconfigure SSinhale.with maxSpeed: 135 @00:45:26.59 SS.seek 25ml @00:45:32.38 Vacuum too high -- waiting up to 40s @00:45:37.54 Intake @27.3psia, vacuum from 20.0 to 24.0psi @00:45:37.56 SS.reconfigure SSinhale.with maxSpeed: 122 @00:45:37.61 SS.seek 25ml @00:45:44.13 Vacuum too high -- waiting up to 40s @00:45:49.47 Intake @27.5psia, vacuum from 20.0 to 24.0psi @00:45:49.49 SS.reconfigure SSinhale.with maxSpeed: 110 @00:45:49.53 SS.seek 25ml @00:45:50.67 Stroke completed -- waiting for pressure to equalize ... @00:45:53.84 Intake.select :close @00:45:54.00 SSV.dial :exhaust @00:45:54.89 Exhaust.select :open @00:45:55.00 SS.reconfigure SSexhale @00:45:55.06 SS.seek 0ml @00:46:04.40 Exhaust.select :close @00:46:04.52 SSV.dial CBV @00:46:04.54 CBV.dial SSV @00:46:05.43 Allowing 130s for 25.0ml of remaining 1475.0ml @00:46:05.44 Intake.select :open @00:46:07.63 Intake @29.8psia, vacuum from 20.0 to 24.0psi @00:46:07.65 SS.reconfigure SSinhale.with maxSpeed: 110 @00:46:07.70 SS.seek 25ml @00:46:10.94 Vacuum too high -- waiting up to 40s @00:46:12.04 Intake @27.1psia, vacuum from 20.0 to 24.0psi @00:46:12.06 SS.reconfigure SSinhale.with maxSpeed: 99 @00:46:12.11 SS.seek 25ml @00:46:19.00 Vacuum too high -- waiting up to 40s @00:46:21.25 delay 5 seconds @00:46:22.89 Intake @27.5psia, vacuum from 20.0 to 24.0psi @00:46:22.91 SS.reconfigure SSinhale.with maxSpeed: 89 @00:46:22.95 SS.seek 25ml @00:46:26.28 ATV.dial :air2 @00:46:27.36 AS.seek 0.52ml @00:46:31.13 Vacuum too high -- waiting up to 40s @00:46:34.76 Intake @27.5psia, vacuum from 20.0 to 24.0psi @00:46:34.78 SS.reconfigure SSinhale.with maxSpeed: 80 @00:46:34.82 SS.seek 25ml @00:46:37.65 Stroke completed -- waiting for pressure to equalize ... @00:46:40.23 Intake.select :close @00:46:40.39 SSV.dial :exhaust @00:46:41.80 Exhaust.select :open @00:46:41.92 SS.reconfigure SSexhale @00:46:41.97 SS.seek 0ml @00:46:50.77 Exhaust.select :close @00:46:50.89 SSV.dial CBV @00:46:50.91 CBV.dial SSV @00:46:51.82 Allowing 130s for 25.0ml of remaining 1450.0ml @00:46:51.83 Intake.select :open @00:46:54.02 Intake @29.8psia, vacuum from 20.0 to 24.0psi @00:46:54.04 SS.reconfigure SSinhale.with maxSpeed: 80 @00:46:54.09 SS.seek 25ml @00:46:58.95 Vacuum too high -- waiting up to 40s @00:46:59.79 Intake @27.3psia, vacuum from 20.0 to 24.0psi @00:46:59.81 SS.reconfigure SSinhale.with maxSpeed: 72 @00:46:59.86 SS.seek 25ml @00:47:15.76 Vacuum too high -- waiting up to 40s @00:47:18.89 Intake @27.5psia, vacuum from 20.0 to 24.0psi @00:47:18.91 SS.reconfigure SSinhale.with maxSpeed: 65 @00:47:18.96 SS.seek 25ml @00:47:24.78 Stroke completed -- waiting for pressure to equalize ... @00:47:27.99 Intake.select :close @00:47:28.15 SSV.dial :exhaust @00:47:29.04 Exhaust.select :open @00:47:29.15 SS.reconfigure SSexhale @00:47:29.21 SS.seek 0ml @00:47:38.69 Exhaust.select :close @00:47:38.81 SSV.dial CBV @00:47:38.83 CBV.dial SSV @00:47:39.71 Allowing 130s for 25.0ml of remaining 1425.0ml @00:47:39.72 Intake.select :open @00:47:41.91 Intake @29.9psia, vacuum from 20.0 to 24.0psi @00:47:41.92 SS.reconfigure SSinhale.with maxSpeed: 65 @00:47:41.97 SS.seek 25ml @00:47:48.97 Vacuum too high -- waiting up to 40s @00:47:49.80 Intake @27.6psia, vacuum from 20.0 to 24.0psi @00:47:49.82 SS.reconfigure SSinhale.with maxSpeed: 59 @00:47:49.87 SS.seek 25ml @00:48:08.23 delay 5 seconds @00:48:13.09 Stroke completed -- waiting for pressure to equalize ... @00:48:13.25 ATV.dial ASV @00:48:14.25 ASV.dial :wasteR @00:48:14.64 AS.seek 0.3ml @00:48:16.98 Intake.select :close @00:48:17.14 SSV.dial :exhaust @00:48:18.56 Exhaust.select :open @00:48:18.68 SS.reconfigure SSexhale @00:48:18.73 SS.seek 0ml @00:48:28.10 Exhaust.select :close @00:48:28.22 SSV.dial CBV @00:48:28.24 CBV.dial SSV @00:48:29.15 Allowing 130s for 25.0ml of remaining 1400.0ml @00:48:29.16 Intake.select :open @00:48:31.35 Intake @30.0psia, vacuum from 20.0 to 24.0psi @00:48:31.37 SS.reconfigure SSinhale.with maxSpeed: 59 @00:48:31.42 SS.seek 25ml @00:48:41.09 Vacuum too high -- waiting up to 40s @00:48:41.93 Intake @27.7psia, vacuum from 20.0 to 24.0psi @00:48:41.95 SS.reconfigure SSinhale.with maxSpeed: 53 @00:48:42.01 SS.seek 25ml @00:48:59.15 delay 20 seconds @00:49:05.10 Stroke completed -- waiting for pressure to equalize ... @00:49:07.70 Intake.select :close @00:49:07.86 SSV.dial :exhaust @00:49:08.74 Exhaust.select :open @00:49:08.86 SS.reconfigure SSexhale @00:49:08.91 SS.seek 0ml @00:49:18.24 Exhaust.select :close @00:49:18.36 SSV.dial CBV @00:49:18.38 CBV.dial SSV @00:49:19.18 AS.seek 0.07ml @00:49:19.27 Allowing 130s for 25.0ml of remaining 1375.0ml @00:49:19.28 Intake.select :open @00:49:21.47 Intake @29.9psia, vacuum from 20.0 to 24.0psi @00:49:21.49 SS.reconfigure SSinhale.with maxSpeed: 53 @00:49:21.53 SS.seek 25ml @00:49:54.93 Stroke completed -- waiting for pressure to equalize ... @00:49:58.15 Intake.select :close @00:49:58.31 SSV.dial :exhaust @00:49:59.18 Exhaust.select :open @00:49:59.29 SS.reconfigure SSexhale @00:49:59.34 SS.seek 0ml @00:50:05.64 delay 20 seconds @00:50:08.37 Exhaust.select :close @00:50:08.49 SSV.dial CBV @00:50:08.52 CBV.dial SSV @00:50:09.43 Allowing 130s for 25.0ml of remaining 1350.0ml @00:50:09.44 Intake.select :open @00:50:11.63 Intake @30.0psia, vacuum from 20.0 to 24.0psi @00:50:11.64 SS.reconfigure SSinhale.with maxSpeed: 53 @00:50:11.69 SS.seek 25ml @00:50:25.66 ATV.dial :waste2 @00:50:26.45 AS.reconfigure ASconfig @00:50:26.52 AS.seek :empty @00:50:30.88 Elution @00:50:30.89 ATV.dial :air1 @00:50:32.07 AS.reconfigure ASslow1 @00:50:32.12 AS.seek 0.049ml @00:50:39.42 Vacuum too high -- waiting up to 40s @00:50:40.25 Intake @27.7psia, vacuum from 20.0 to 24.0psi @00:50:40.27 SS.reconfigure SSinhale.with maxSpeed: 48 @00:50:40.32 SS.seek 25ml @00:50:42.51 delay 3 seconds @00:50:45.53 ATV.dial :ElutionWater @00:50:46.22 AS.seek 0.109ml @00:50:48.03 Stroke completed -- waiting for pressure to equalize ... @00:50:50.54 Intake.select :close @00:50:50.70 SSV.dial :exhaust @00:50:51.57 Exhaust.select :open @00:50:51.69 SS.reconfigure SSexhale @00:50:51.74 SS.seek 0ml @00:50:58.62 delay 3 seconds @00:51:00.56 Exhaust.select :close @00:51:00.68 SSV.dial CBV @00:51:00.71 CBV.dial SSV @00:51:01.60 Allowing 130s for 25.0ml of remaining 1325.0ml @00:51:01.61 Intake.select :open @00:51:01.67 ATV.dial ASV,via: :air2 @00:51:03.80 Intake @29.7psia, vacuum from 20.0 to 24.0psi @00:51:03.82 SS.reconfigure SSinhale.with maxSpeed: 48 @00:51:03.88 SS.seek 25ml @00:51:03.95 ASV.dial :SPEelution @00:51:04.56 AFV.select :carrierSolution @00:51:04.72 AS.reconfigure ASnorm @00:51:04.78 AS.seek 0.759ml @00:51:37.92 delay 3 seconds @00:51:40.95 AFV.select :holdingCoil @00:51:41.10 AS.reconfigure ASslow1 @00:51:41.16 AS.seek 0.474ml @00:51:41.59 Stroke completed -- waiting for pressure to equalize ... @00:51:44.20 Intake.select :close @00:51:44.36 SSV.dial :exhaust @00:51:45.24 Exhaust.select :open @00:51:45.35 SS.reconfigure SSexhale @00:51:45.40 SS.seek 0ml @00:51:54.43 Exhaust.select :close @00:51:54.54 SSV.dial CBV @00:51:54.57 CBV.dial SSV @00:51:55.47 Allowing 130s for 25.0ml of remaining 1300.0ml @00:51:55.48 Intake.select :open @00:51:57.67 Intake @29.7psia, vacuum from 20.0 to 24.0psi @00:51:57.69 SS.reconfigure SSinhale.with maxSpeed: 48 @00:51:57.74 SS.seek 25ml @00:52:35.45 Stroke completed -- waiting for pressure to equalize ... @00:52:38.05 Intake.select :close @00:52:38.21 SSV.dial :exhaust @00:52:38.78 delay 5 seconds @00:52:39.10 Exhaust.select :open @00:52:39.21 SS.reconfigure SSexhale @00:52:39.26 SS.seek 0ml @00:52:43.86 AS.reconfigure ASslow2 @00:52:43.91 AS.seek 0.439ml @00:52:48.70 Exhaust.select :close @00:52:48.82 SSV.dial CBV @00:52:48.86 CBV.dial SSV @00:52:49.75 Allowing 130s for 25.0ml of remaining 1275.0ml @00:52:49.76 Intake.select :open @00:52:51.95 Intake @29.7psia, vacuum from 20.0 to 24.0psi @00:52:51.96 SS.reconfigure SSinhale.with maxSpeed: 48 @00:52:52.01 SS.seek 25ml @00:53:19.25 delay 1:00 @00:53:28.96 Stroke completed -- waiting for pressure to equalize ... @00:53:31.56 Intake.select :close @00:53:31.72 SSV.dial :exhaust @00:53:32.61 Exhaust.select :open @00:53:32.72 SS.reconfigure SSexhale @00:53:32.77 SS.seek 0ml @00:53:42.10 Exhaust.select :close @00:53:42.22 SSV.dial CBV @00:53:42.25 CBV.dial SSV @00:53:43.13 Allowing 130s for 25.0ml of remaining 1250.0ml @00:53:43.14 Intake.select :open @00:53:45.32 Intake @29.8psia, vacuum from 20.0 to 24.0psi @00:53:45.86 SS.reconfigure SSinhale.with maxSpeed: 48 @00:53:45.93 SS.seek 25ml @00:54:19.27 pushing eluate to holding coil @00:54:19.34 AS.seek 0.279ml @00:54:22.99 Stroke completed -- waiting for pressure to equalize ... @00:54:26.24 Intake.select :close @00:54:26.40 SSV.dial :exhaust @00:54:27.27 Exhaust.select :open @00:54:27.38 SS.reconfigure SSexhale @00:54:27.44 SS.seek 0ml @00:54:36.77 Exhaust.select :close @00:54:36.88 SSV.dial CBV @00:54:36.91 CBV.dial SSV @00:54:37.80 Allowing 130s for 25.0ml of remaining 1225.0ml @00:54:37.81 Intake.select :open @00:54:39.99 Intake @29.8psia, vacuum from 20.0 to 24.0psi @00:54:40.01 SS.reconfigure SSinhale.with maxSpeed: 48 @00:54:40.06 SS.seek 25ml @00:55:17.10 Stroke completed -- waiting for pressure to equalize ... @00:55:20.33 Intake.select :close @00:55:20.49 SSV.dial :exhaust @00:55:21.36 Exhaust.select :open @00:55:21.48 SS.reconfigure SSexhale @00:55:21.53 SS.seek 0ml @00:55:31.05 Exhaust.select :close @00:55:31.16 SSV.dial CBV @00:55:31.19 CBV.dial SSV @00:55:32.10 Allowing 130s for 25.0ml of remaining 1200.0ml @00:55:32.11 Intake.select :open @00:55:34.29 Intake @29.8psia, vacuum from 20.0 to 24.0psi @00:55:34.31 SS.reconfigure SSinhale.with maxSpeed: 48 @00:55:34.37 SS.seek 25ml @00:56:11.62 Stroke completed -- waiting for pressure to equalize ... @00:56:14.84 Intake.select :close @00:56:15.00 SSV.dial :exhaust @00:56:15.89 Exhaust.select :open @00:56:16.00 SS.reconfigure SSexhale @00:56:16.05 SS.seek 0ml @00:56:25.49 Exhaust.select :close @00:56:25.61 SSV.dial CBV @00:56:25.64 CBV.dial SSV @00:56:26.53 Allowing 130s for 25.0ml of remaining 1175.0ml @00:56:26.54 Intake.select :open @00:56:28.72 Intake @29.8psia, vacuum from 20.0 to 24.0psi @00:56:28.74 SS.reconfigure SSinhale.with maxSpeed: 48 @00:56:28.79 SS.seek 25ml @00:57:00.48 delay 30 seconds @00:57:05.88 Stroke completed -- waiting for pressure to equalize ... @00:57:09.10 Intake.select :close @00:57:09.27 SSV.dial :exhaust @00:57:10.16 Exhaust.select :open @00:57:10.27 SS.reconfigure SSexhale @00:57:10.32 SS.seek 0ml @00:57:19.83 Exhaust.select :close @00:57:19.94 SSV.dial CBV @00:57:20.42 CBV.dial SSV @00:57:21.41 Allowing 130s for 25.0ml of remaining 1150.0ml @00:57:21.42 Intake.select :open @00:57:23.60 Intake @29.9psia, vacuum from 20.0 to 24.0psi @00:57:23.62 SS.reconfigure SSinhale.with maxSpeed: 48 @00:57:23.67 SS.seek 25ml @00:57:30.51 ATV.dial :waste,via: :air3 @00:57:32.33 AS.reconfigure ASconfig @00:57:32.38 AS.seek :empty @00:57:48.78 Parking Bleach in LysateSource line and SPEcolumn @00:57:48.79 ATV.dial :air3 @00:57:49.66 AS.reconfigure ASslow1 @00:57:49.71 AS.seek 0.029ml @00:57:51.48 Vacuum too high -- waiting up to 40s @00:57:52.33 Intake @27.9psia, vacuum from 20.0 to 24.0psi @00:57:52.35 SS.reconfigure SSinhale.with maxSpeed: 43 @00:57:52.39 SS.seek 25ml @00:57:55.95 ATV.dial :ElutionWater @00:57:56.57 AS.seek 0.059ml @00:58:02.68 Stroke completed -- waiting for pressure to equalize ... @00:58:02.83 ATV.dial :air3 @00:58:03.46 AS.seek 0.069ml @00:58:05.30 Intake.select :close @00:58:05.46 SSV.dial :exhaust @00:58:05.76 delay 2 seconds @00:58:06.35 Exhaust.select :open @00:58:06.47 SS.reconfigure SSexhale @00:58:06.52 SS.seek 0ml @00:58:07.78 ATV.dial :bleach @00:58:08.25 AS.seek 0.769ml @00:58:15.94 Exhaust.select :close @00:58:16.05 SSV.dial CBV @00:58:16.08 CBV.dial SSV @00:58:16.97 Allowing 130s for 25.0ml of remaining 1125.0ml @00:58:16.98 Intake.select :open @00:58:19.17 Intake @29.9psia, vacuum from 20.0 to 24.0psi @00:58:19.19 SS.reconfigure SSinhale.with maxSpeed: 43 @00:58:19.23 SS.seek 25ml @00:59:00.19 Stroke completed -- waiting for pressure to equalize ... @00:59:02.78 Intake.select :close @00:59:02.94 SSV.dial :exhaust @00:59:03.81 Exhaust.select :open @00:59:03.92 SS.reconfigure SSexhale @00:59:03.97 SS.seek 0ml @00:59:13.52 Exhaust.select :close @00:59:13.63 SSV.dial CBV @00:59:13.66 CBV.dial SSV @00:59:14.55 Allowing 130s for 25.0ml of remaining 1100.0ml @00:59:14.56 Intake.select :open @00:59:16.74 Intake @29.8psia, vacuum from 20.0 to 24.0psi @00:59:16.76 SS.reconfigure SSinhale.with maxSpeed: 43 @00:59:16.81 SS.seek 25ml @00:59:57.85 Stroke completed -- waiting for pressure to equalize ... @01:00:00.43 Intake.select :close @01:00:00.59 SSV.dial :exhaust @01:00:01.45 Exhaust.select :open @01:00:01.57 SS.reconfigure SSexhale @01:00:01.61 SS.seek 0ml @01:00:10.99 Exhaust.select :close @01:00:11.10 SSV.dial CBV @01:00:11.59 CBV.dial SSV @01:00:12.56 Allowing 130s for 25.0ml of remaining 1075.0ml @01:00:12.57 Intake.select :open @01:00:14.75 Intake @29.8psia, vacuum from 20.0 to 24.0psi @01:00:14.77 SS.reconfigure SSinhale.with maxSpeed: 43 @01:00:14.82 SS.seek 25ml @01:00:29.28 delay 3 seconds @01:00:32.31 ATV.dial :air3 @01:00:32.87 delay 3 seconds @01:00:35.96 AS.seek 0.794ml @01:00:41.28 delay 3 seconds @01:00:44.31 ATV.dial :bleach @01:00:44.94 AS.seek 0.779ml @01:00:48.25 delay 3 seconds @01:00:51.28 ATV.dial :AIV @01:00:52.59 AS.reconfigure ASconfig @01:00:52.65 AS.seek 0.015ml @01:00:56.27 Stroke completed -- waiting for pressure to equalize ... @01:00:59.51 Intake.select :close @01:00:59.67 SSV.dial :exhaust @01:01:00.56 Exhaust.select :open @01:01:00.68 SS.reconfigure SSexhale @01:01:00.72 SS.seek 0ml @01:01:10.06 Exhaust.select :close @01:01:10.18 SSV.dial CBV @01:01:10.20 CBV.dial SSV @01:01:11.10 Allowing 130s for 25.0ml of remaining 1050.0ml @01:01:11.11 Intake.select :open @01:01:13.30 Intake @29.8psia, vacuum from 20.0 to 24.0psi @01:01:13.31 SS.reconfigure SSinhale.with maxSpeed: 43 @01:01:13.36 SS.seek 25ml @01:01:36.85 ATV.dial :waste @01:01:38.42 AS.seek :empty @01:01:39.65 ATV.dial :air3 @01:01:40.35 AS.reconfigure ASslow1 @01:01:40.40 AS.seek 0.009ml @01:01:42.79 delay 2 seconds @01:01:44.81 ATV.dial :bleach @01:01:45.27 AS.seek 0.579ml @01:01:54.71 Stroke completed -- waiting for pressure to equalize ... @01:01:57.92 Intake.select :close @01:01:58.09 SSV.dial :exhaust @01:01:58.97 Exhaust.select :open @01:01:59.09 SS.reconfigure SSexhale @01:01:59.14 SS.seek 0ml @01:02:08.59 Exhaust.select :close @01:02:08.70 SSV.dial CBV @01:02:08.73 CBV.dial SSV @01:02:09.61 Allowing 130s for 25.0ml of remaining 1025.0ml @01:02:09.62 Intake.select :open @01:02:11.81 Intake @29.9psia, vacuum from 20.0 to 24.0psi @01:02:11.83 SS.reconfigure SSinhale.with maxSpeed: 43 @01:02:11.87 SS.seek 25ml @01:02:53.01 Stroke completed -- waiting for pressure to equalize ... @01:02:56.23 Intake.select :close @01:02:56.39 SSV.dial :exhaust @01:02:57.27 Exhaust.select :open @01:02:57.39 SS.reconfigure SSexhale @01:02:57.43 SS.seek 0ml @01:03:06.74 Exhaust.select :close @01:03:06.86 SSV.dial CBV @01:03:06.89 CBV.dial SSV @01:03:08.31 Allowing 130s for 25.0ml of remaining 1000.0ml @01:03:08.32 Intake.select :open @01:03:10.51 Intake @29.7psia, vacuum from 20.0 to 24.0psi @01:03:10.53 SS.reconfigure SSinhale.with maxSpeed: 43 @01:03:10.57 SS.seek 25ml @01:03:40.15 delay 3 seconds @01:03:43.18 ATV.dial :air3 @01:03:43.75 delay 3 seconds @01:03:46.84 AS.seek 0.604ml @01:03:51.99 Stroke completed -- waiting for pressure to equalize ... @01:03:52.15 delay 3 seconds @01:03:55.22 ATV.dial :bleach @01:03:55.29 Intake.select :close @01:03:55.45 SSV.dial :exhaust @01:03:55.85 AS.seek 0.589ml @01:03:56.33 Exhaust.select :open @01:03:56.44 SS.reconfigure SSexhale @01:03:56.50 SS.seek 0ml @01:03:59.15 delay 3 seconds @01:04:02.18 ATV.dial :ASV @01:04:03.25 ASV.dial :wasteR @01:04:03.94 AS.seek 0ml @01:04:05.88 Exhaust.select :close @01:04:06.00 SSV.dial CBV @01:04:06.02 CBV.dial SSV @01:04:06.92 Allowing 130s for 25.0ml of remaining 975.0ml @01:04:06.93 Intake.select :open @01:04:09.12 Intake @29.8psia, vacuum from 20.0 to 24.0psi @01:04:09.14 SS.reconfigure SSinhale.with maxSpeed: 43 @01:04:09.18 SS.seek 25ml @01:04:50.35 Stroke completed -- waiting for pressure to equalize ... @01:04:54.21 Intake.select :close @01:04:54.37 SSV.dial :exhaust @01:04:55.23 Exhaust.select :open @01:04:55.34 SS.reconfigure SSexhale @01:04:55.41 SS.seek 0ml @01:05:04.84 Exhaust.select :close @01:05:04.95 SSV.dial CBV @01:05:04.98 CBV.dial SSV @01:05:05.87 Allowing 130s for 25.0ml of remaining 950.0ml @01:05:05.88 Intake.select :open @01:05:08.07 Intake @29.7psia, vacuum from 20.0 to 24.0psi @01:05:08.09 SS.reconfigure SSinhale.with maxSpeed: 43 @01:05:08.14 SS.seek 25ml @01:05:49.30 Stroke completed -- waiting for pressure to equalize ... @01:05:53.15 Intake.select :close @01:05:53.31 SSV.dial :exhaust @01:05:54.20 Exhaust.select :open @01:05:54.31 SS.reconfigure SSexhale @01:05:54.38 SS.seek 0ml @01:06:02.73 delay 1:00 @01:06:03.97 Exhaust.select :close @01:06:04.09 SSV.dial CBV @01:06:04.12 CBV.dial SSV @01:06:05.00 Allowing 130s for 25.0ml of remaining 925.0ml @01:06:05.01 Intake.select :open @01:06:07.19 Intake @29.9psia, vacuum from 20.0 to 24.0psi @01:06:07.21 SS.reconfigure SSinhale.with maxSpeed: 43 @01:06:07.26 SS.seek 25ml @01:06:48.71 Stroke completed -- waiting for pressure to equalize ... @01:06:52.50 Intake.select :close @01:06:52.66 SSV.dial :exhaust @01:06:53.53 Exhaust.select :open @01:06:53.65 SS.reconfigure SSexhale @01:06:53.71 SS.seek 0ml @01:07:02.76 AFV.select :carrierSolution @01:07:02.86 delay 2 seconds @01:07:03.23 Exhaust.select :close @01:07:03.34 SSV.dial CBV @01:07:03.37 CBV.dial SSV @01:07:04.26 Allowing 130s for 25.0ml of remaining 900.0ml @01:07:04.27 Intake.select :open @01:07:04.88 AS.reconfigure ASconfig @01:07:04.93 AS.seek 1ml @01:07:06.46 Intake @29.8psia, vacuum from 20.0 to 24.0psi @01:07:06.48 SS.reconfigure SSinhale.with maxSpeed: 43 @01:07:06.53 SS.seek 25ml @01:07:40.62 Vacuum too high -- waiting up to 40s @01:07:41.47 Intake @28.0psia, vacuum from 20.0 to 24.0psi @01:07:41.49 SS.reconfigure SSinhale.with maxSpeed: 39 @01:07:41.54 SS.seek 25ml @01:07:49.25 Stroke completed -- waiting for pressure to equalize ... @01:07:51.83 Intake.select :close @01:07:52.00 SSV.dial :exhaust @01:07:52.89 Exhaust.select :open @01:07:53.01 SS.reconfigure SSexhale @01:07:53.06 SS.seek 0ml @01:08:02.49 Exhaust.select :close @01:08:02.61 SSV.dial CBV @01:08:02.64 CBV.dial SSV @01:08:02.71 delay 1 second @01:08:03.56 Allowing 130s for 25.0ml of remaining 875.0ml @01:08:03.57 Intake.select :open @01:08:03.75 AFV.select :holdingCoil @01:08:03.85 delay 1 second @01:08:04.88 ATV.dial ASV @01:08:04.89 ASV.dial :wasteR @01:08:04.91 AS.reconfigure ASslow1 @01:08:04.95 AS.seek 0ml @01:08:05.76 Intake @29.9psia, vacuum from 20.0 to 24.0psi @01:08:05.78 SS.reconfigure SSinhale.with maxSpeed: 39 @01:08:05.82 SS.seek 25ml @01:08:51.08 Stroke completed -- waiting for pressure to equalize ... @01:08:54.28 Intake.select :close @01:08:54.45 SSV.dial :exhaust @01:08:55.33 Exhaust.select :open @01:08:55.44 SS.reconfigure SSexhale @01:08:55.49 SS.seek 0ml @01:09:04.97 Exhaust.select :close @01:09:05.09 SSV.dial CBV @01:09:05.11 CBV.dial SSV @01:09:06.01 Allowing 130s for 25.0ml of remaining 850.0ml @01:09:06.02 Intake.select :open @01:09:08.21 Intake @29.8psia, vacuum from 20.0 to 24.0psi @01:09:08.23 SS.reconfigure SSinhale.with maxSpeed: 39 @01:09:08.28 SS.seek 25ml @01:09:53.41 Stroke completed -- waiting for pressure to equalize ... @01:09:56.62 Intake.select :close @01:09:57.33 SSV.dial :exhaust @01:09:58.22 Exhaust.select :open @01:09:58.34 SS.reconfigure SSexhale @01:09:58.39 SS.seek 0ml @01:10:07.76 Exhaust.select :close @01:10:07.87 SSV.dial CBV @01:10:07.90 CBV.dial SSV @01:10:08.78 Allowing 130s for 25.0ml of remaining 825.0ml @01:10:08.79 Intake.select :open @01:10:10.98 Intake @29.7psia, vacuum from 20.0 to 24.0psi @01:10:11.00 SS.reconfigure SSinhale.with maxSpeed: 39 @01:10:11.04 SS.seek 25ml @01:10:56.36 Stroke completed -- waiting for pressure to equalize ... @01:11:00.24 Intake.select :close @01:11:00.40 SSV.dial :exhaust @01:11:01.28 Exhaust.select :open @01:11:01.40 SS.reconfigure SSexhale @01:11:01.46 SS.seek 0ml @01:11:11.00 Exhaust.select :close @01:11:11.11 SSV.dial CBV @01:11:11.14 CBV.dial SSV @01:11:12.03 Allowing 130s for 25.0ml of remaining 800.0ml @01:11:12.04 Intake.select :open @01:11:14.23 Intake @29.6psia, vacuum from 20.0 to 24.0psi @01:11:14.25 SS.reconfigure SSinhale.with maxSpeed: 39 @01:11:14.29 SS.seek 25ml @01:11:26.34 delay 20 seconds @01:11:46.37 ATV.dial :air3 @01:11:47.97 AS.seek 0.59ml @01:11:59.30 Stroke completed -- waiting for pressure to equalize ... @01:12:02.51 Intake.select :close @01:12:02.67 SSV.dial :exhaust @01:12:03.56 Exhaust.select :open @01:12:03.67 SS.reconfigure SSexhale @01:12:03.72 SS.seek 0ml @01:12:13.12 Exhaust.select :close @01:12:13.23 SSV.dial CBV @01:12:13.26 CBV.dial SSV @01:12:14.14 Allowing 130s for 25.0ml of remaining 775.0ml @01:12:14.15 Intake.select :open @01:12:16.34 Intake @29.6psia, vacuum from 20.0 to 24.0psi @01:12:16.36 SS.reconfigure SSinhale.with maxSpeed: 39 @01:12:16.41 SS.seek 25ml @01:13:01.78 Stroke completed -- waiting for pressure to equalize ... @01:13:05.62 Intake.select :close @01:13:05.78 SSV.dial :exhaust @01:13:06.65 Exhaust.select :open @01:13:06.77 SS.reconfigure SSexhale @01:13:06.83 SS.seek 0ml @01:13:16.19 Exhaust.select :close @01:13:16.31 SSV.dial CBV @01:13:16.34 CBV.dial SSV @01:13:17.25 Allowing 130s for 25.0ml of remaining 750.0ml @01:13:17.26 Intake.select :open @01:13:19.44 Intake @29.7psia, vacuum from 20.0 to 24.0psi @01:13:19.46 SS.reconfigure SSinhale.with maxSpeed: 39 @01:13:19.51 SS.seek 25ml @01:13:46.93 delay 3 seconds @01:13:49.96 ATV.dial :ASV @01:13:51.45 ASV.dial :wasteR @01:13:52.08 AS.seek 0.09ml @01:14:04.80 Stroke completed -- waiting for pressure to equalize ... @01:14:08.71 Intake.select :close @01:14:08.87 SSV.dial :exhaust @01:14:09.75 Exhaust.select :open @01:14:09.87 SS.reconfigure SSexhale @01:14:09.93 SS.seek 0ml @01:14:18.90 Exhaust.select :close @01:14:19.02 SSV.dial CBV @01:14:19.04 CBV.dial SSV @01:14:19.93 Allowing 130s for 25.0ml of remaining 725.0ml @01:14:19.94 Intake.select :open @01:14:22.13 Intake @29.4psia, vacuum from 20.0 to 24.0psi @01:14:22.15 SS.reconfigure SSinhale.with maxSpeed: 39 @01:14:22.19 SS.seek 25ml @01:15:08.61 Stroke completed -- waiting for pressure to equalize ... @01:15:12.45 Intake.select :close @01:15:12.62 SSV.dial :exhaust @01:15:13.50 Exhaust.select :open @01:15:13.61 SS.reconfigure SSexhale @01:15:13.67 SS.seek 0ml @01:15:23.21 Exhaust.select :close @01:15:23.32 SSV.dial CBV @01:15:23.35 CBV.dial SSV @01:15:24.25 Allowing 130s for 25.0ml of remaining 700.0ml @01:15:24.26 Intake.select :open @01:15:26.45 Intake @29.8psia, vacuum from 20.0 to 24.0psi @01:15:26.47 SS.reconfigure SSinhale.with maxSpeed: 39 @01:15:26.52 SS.seek 25ml @01:15:32.92 delay 30 seconds @01:15:53.96 Vacuum too high -- waiting up to 40s @01:15:54.79 Intake @28.0psia, vacuum from 20.0 to 24.0psi @01:15:54.81 SS.reconfigure SSinhale.with maxSpeed: 35 @01:15:54.86 SS.seek 25ml @01:16:02.95 ATV.dial :extSrc @01:16:03.76 delay 3 seconds @01:16:06.79 ATV.dial :ElutionWater @01:16:07.97 AS.seek 0.12ml @01:16:14.36 ATV.dial :air3 @01:16:14.63 Stroke completed -- waiting for pressure to equalize ... @01:16:15.00 AS.seek 0.13ml @01:16:17.26 delay 2 seconds @01:16:17.86 Intake.select :close @01:16:18.03 SSV.dial :exhaust @01:16:18.92 Exhaust.select :open @01:16:19.03 SS.reconfigure SSexhale @01:16:19.09 SS.seek 0ml @01:16:19.28 ATV.dial :bleach @01:16:19.76 AS.seek 0.23ml @01:16:28.49 Exhaust.select :close @01:16:28.63 SSV.dial CBV @01:16:28.65 CBV.dial SSV @01:16:30.08 Allowing 130s for 25.0ml of remaining 675.0ml @01:16:30.12 Intake.select :open @01:16:32.30 Intake @29.6psia, vacuum from 20.0 to 24.0psi @01:16:32.32 SS.reconfigure SSinhale.with maxSpeed: 35 @01:16:32.37 SS.seek 25ml @01:16:40.17 delay 3 seconds @01:16:43.20 ATV.dial :air3 @01:16:43.76 delay 3 seconds @01:16:46.85 AS.seek 0.255ml @01:16:52.17 delay 3 seconds @01:16:55.20 ATV.dial :bleach @01:16:55.83 AS.seek 0.24ml @01:16:59.14 delay 3 seconds @01:17:02.17 ATV.dial :ASV @01:17:03.28 ASV.dial :SPEdelivery @01:17:03.67 AFV.select :carrierSolution @01:17:03.78 delay 2 seconds @01:17:05.81 AS.reconfigure ASconfig @01:17:05.86 AS.seek 0.5ml @01:17:21.12 delay 1 second @01:17:22.14 AFV.select :holdingCoil @01:17:22.29 AS.reconfigure ASslow1 @01:17:22.35 AS.seek 0.17ml @01:17:22.86 Stroke completed -- waiting for pressure to equalize ... @01:17:26.10 Intake.select :close @01:17:26.27 SSV.dial :exhaust @01:17:27.15 Exhaust.select :open @01:17:27.26 SS.reconfigure SSexhale @01:17:27.31 SS.seek 0ml @01:17:36.82 Exhaust.select :close @01:17:36.93 SSV.dial CBV @01:17:36.96 CBV.dial SSV @01:17:37.85 Allowing 130s for 25.0ml of remaining 650.0ml @01:17:37.86 Intake.select :open @01:17:40.04 Intake @29.6psia, vacuum from 20.0 to 24.0psi @01:17:40.06 SS.reconfigure SSinhale.with maxSpeed: 35 @01:17:40.11 SS.seek 25ml @18:03:06.16CEST08-Sep-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @18:03:08.22 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @18:03:08.52 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @18:03:13.77 Polling Can every 10:00 @18:03:15.92 SPE.coast @18:03:16.04 SPE.configure SPEconfig @18:03:16.23 AS.reconfigure ASconfig @18:03:16.32 AS.hold @18:03:16.44 AS.configure ASconfig @18:03:16.64 SS.configure SSconfig @18:03:16.84 CC.reconfigure CCconfig @18:03:16.93 CC.hold @18:03:17.04 CC.configure CCconfig @18:03:17.23 CS.configure CSconfig @18:03:17.43 PC.reconfigure PCconfig @18:03:17.51 PC.hold @18:03:17.64 PC.configure PCconfig @18:03:18.53 PS.reconfigure PSconfig @18:03:18.66 PS.hold @18:03:18.79 PS.configure PSconfig @18:03:19.03 Forearm.reconfigure LooseForearm @18:03:19.11 Forearm.hold @18:03:19.23 Forearm.configure LooseForearm @18:03:19.41 Elbow.reconfigure LooseElbow @18:03:19.50 Elbow.hold @18:03:19.62 Elbow.configure LooseElbow @18:03:19.86 SC.reconfigure SCloose @18:03:19.95 SC.hold @18:03:20.06 SC.configure SCloose @18:03:20.26 SE.reconfigure SEconfig @18:03:20.34 SE.hold @18:03:20.46 SE.configure SEconfig @18:03:20.55 CH.coast @18:03:20.67 CH.configure CHconfig @18:03:20.73 PH.coast @18:03:20.85 PH.configure PHconfig @18:03:20.93 PCR.off @18:03:20.98 PCR.pidtemp= 50,0.1,0,300,25,25 @18:03:21.04 PCR.caltemp= 2.7073,0.0373,0 @18:03:21.08 All dwarf controllers running firmware version 3.75 @18:03:21.28 Loading /home/esp/esp2/mission/18tun1.rb mission @18:03:21.40 Preparing to sleep until +0:00:15 @18:03:21.42 GoodNews.email "Starting 18tun1 w/pucks in tube 2\n{}\nCan@18:03:13, 23.7C, 46% humidity, 14.6psia, 11.992V, 0.727A, 0.727A avg, 8.71W",Subject: "18tun1 begun" @18:03:21.50 AS.coast @18:03:21.53
Asleep til +0:00:15 @18:03:21.57 Continuing at 18:03 @18:03:21.60 CC.coast @18:03:21.70 PC.coast @18:03:21.74 PS.coast @18:03:21.78 Forearm.coast @18:03:21.81 Elbow.coast @18:03:21.85 SC.coast @18:03:21.89 SE.coast @18:03:22.28 Gate.power :core,:OFF @18:03:22.30 Gate.power :raw,:OFF @18:03:22.31 Shutdown @18:03:30.44 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:106\n{}\nCan@18:03:21, 23.7C, 46% humidity, 14.6psia, 11.972V, 0.802A, 0.802A avg, 9.60W",Subject: "Asleep until 18:03" @18:03:36.30
Powering core bus @18:03:41.37 Gate.power :raw,:ON @18:03:42.10 SPE.coast @18:03:42.17 SPE.configure SPEconfig @18:03:42.26 AS.configure ASconfig @18:03:42.38 SS.configure SSconfig @18:03:42.49 CC.configure CCconfig @18:03:42.59 CS.configure CSconfig @18:03:42.69 PC.configure PCconfig @18:03:42.80 PS.configure PSconfig @18:03:42.95 Forearm.configure LooseForearm @18:03:43.05 Elbow.configure LooseElbow @18:03:43.19 SC.configure SCloose @18:03:43.28 SE.configure SEconfig @18:03:43.33 CH.coast @18:03:43.39 CH.configure CHconfig @18:03:43.42 PH.coast @18:03:43.49 PH.configure PHconfig @18:03:43.53 PCR.off @18:03:43.56 PCR.pidtemp= 50,0.1,0,300,25,25 @18:03:43.59 PCR.caltemp= 2.7073,0.0373,0 @18:03:43.62 All dwarf controllers running firmware version 3.75 @18:03:43.74 Valve.reset @18:03:43.93 PCR.off @18:03:43.96 PCR.pidtemp= 50,0.1,0,300,25,25 @18:03:43.98 PCR.caltemp= 2.7073,0.0373,0 @18:03:44.21 Intake.select :close @18:03:44.22 Exhaust.select :close @18:03:44.24 CTV.dial :air @18:03:44.33 PSV.dial :air @18:03:44.40 CSV.dial :waste1 @18:03:44.54
Forearm.reconfigure LooseForearm @18:03:44.58 Forearm.home.seek :retract @18:03:45.29 PS.reconfigure PSconfig @18:03:45.37 Hand.close @18:03:45.42 SSV.dial :spit,avoiding: :exhaust @18:03:45.45 PS.home.seek 0ml @18:03:45.88
Elbow.jog 1500 @18:03:46.11 CS.reconfigure CSfast @18:03:46.15 SS.reconfigure SSconfig @18:03:46.25 CS.home.seek 0ml @18:03:46.28 SS.home.seek 0ml @18:03:46.47
Elbow.reconfigure LooseElbow @18:03:46.52 Elbow.home.seek :clear @18:03:47.36 Session Begun @18:03:50.85 -> showlog 100 @18:03:51.18
SE.reconfigure SEconfig @18:03:51.25 SE.home.seek :down @18:03:51.99 SC.reconfigure SCsloppy @18:03:52.07 SC.home.seek :initial @18:03:55.19 PS.coast @18:04:02.40
Forearm.seek :retract @18:04:02.54 Elbow.seek :hammer @18:04:03.72 Forearm.reconfigure TightForearm @18:04:03.80 Forearm.seek 5 + 350 counts @18:04:04.86 Forearm.seek :retract @18:04:06.08 PC.reconfigure PCconfig @18:04:06.09
CC.reconfigure CCconfig @18:04:06.21 PC.home.seek :open @18:04:06.25
CC.home.seek :open @18:04:09.64 Phase [mbaPhase] @18:04:09.65 GoodNews.email "At 18:04:09 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:106\n{}\nCan@18:04:09, 23.6C, 46% humidity, 14.6psia, 11.898V, 1.080A, 1.080A avg, 12.85W",Subject: "Started 18tun1 phase mbaPhase" @18:04:09.69CEST08-Sep-18
==================> STARTED MBAsuperphase (phase #1) <=== @18:04:09.73 Starting initialPurge @18:04:09.76 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:04:09.86 Hand.open @18:04:10.72 Elbow.reconfigure TightElbow @18:04:10.80 Elbow.seek FlushPuck::Garage @18:04:12.85 Elbow.reconfigure LooseElbow @18:04:12.98 Forearm.reconfigure LooseForearm @18:04:13.06 Forearm.seek :garage @18:04:14.10 Hand.close @18:04:15.09 Forearm.seek :retract @18:04:16.16 Elbow.reconfigure TightElbow @18:04:16.24 Elbow.seek CC @18:04:19.21 Elbow.reconfigure LooseElbow @18:04:19.33 Forearm.reconfigure TightForearm @18:04:19.42 Forearm.seek CC @18:04:20.74 CC.seek :closed @18:04:25.11 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.43 @18:04:25.21 Hand.open @18:04:26.00 Forearm.reconfigure LooseForearm @18:04:26.08 Forearm.seek :retract @18:04:27.07 Flushing the Collection Valve::Manifold @18:04:27.23 CSV.dial CSR @18:04:28.78 Pulling 5ml of CSR.flush @18:04:28.79 CSR.select :flush @18:04:28.95 CS.reconfigure CSconfig @18:04:29.02 CS.seek 5ml @18:04:42.96 delay 5 seconds @18:04:47.98 CSR.select :air @18:04:48.17 CS.reconfigure CSfast @18:04:48.24 CS.seek 7.5ml @18:04:55.64 CSV.dial :waste @18:04:57.15 CS.reconfigure CSconfig @18:04:57.22 CS.seek 0ml @18:05:17.62 Purging external Intake @18:05:17.77 CSV.dial :flush @18:05:18.30 CS.reconfigure CSfast @18:05:18.37 CS.seek 10ml @18:05:45.12 delay 5 seconds @18:05:50.15 CSV.dial CBV @18:05:50.18
CBV.dial CSV @18:05:51.43 CTV.dial :intake @18:05:52.30 Intake.select :open @18:05:52.44 CS.seek 0ml @18:06:20.17 Intake.select :close @18:06:20.31 CTV.dial :air @18:06:21.18 Flushing upper manifold @18:06:21.34 CSV.dial :flush @18:06:22.20 CS.seek 5ml @18:06:35.93 delay 5 seconds @18:06:40.95 CSV.dial :air @18:06:41.55 CS.seek 7ml @18:06:47.62 CSV.dial CBV @18:06:47.64
CBV.dial CSV @18:06:48.83 CTV.dial :air @18:06:48.86 CS.seek 0ml @18:07:07.80 delay 7 seconds @18:07:14.84 CS.seek 3ml @18:07:23.48 CSV.dial :waste @18:07:24.14 CS.seek 0ml @18:07:32.99 CSV.dial :flush @18:07:33.97 CS.seek 3ml @18:07:42.73 delay 5 seconds @18:07:47.75 CSV.dial :air @18:07:48.47 CS.seek 5ml @18:07:54.54 CSV.dial :air @18:07:54.57 CS.seek 0ml @18:08:08.68 delay 7 seconds @18:08:15.72 CS.seek 3ml @18:08:24.33 CSV.dial :waste @18:08:25.20 CS.seek 0ml @18:08:33.87 Garaging FlushPuck in Collection Clamp @18:08:33.97 Elbow.reconfigure TightElbow @18:08:34.05 Elbow.seek CC @18:08:34.32 Elbow.reconfigure LooseElbow @18:08:34.46 Forearm.seek CC @18:08:35.46 Hand.close @18:08:36.21 CC.seek :open @18:08:40.62 Forearm.seek :retract @18:08:41.65 Elbow.reconfigure TightElbow @18:08:41.72 Elbow.seek :garage @18:08:45.05 Elbow.reconfigure LooseElbow @18:08:45.18 Forearm.reconfigure TightForearm @18:08:45.25 Forearm.seek :garage @18:08:46.42 Hand.open @18:08:47.26 Forearm.reconfigure LooseForearm @18:08:47.34 Forearm.seek :retract @18:08:48.46 Starting mfb @18:08:48.48 pcrBAC sampling at most 1500ml, wcr at most 1500ml @18:08:48.53 BACfans.email "Started mfb in tube 2 at 18:08:48CEST08-Sep-18\n{}\nCan@18:04:09, 23.6C, 46% humidity, 14.6psia, 11.898V, 1.080A, 1.080A avg, 12.85W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @18:08:48.59
Loading sh1 puck from tube 2 into Collection Clamp @18:08:48.80 Readying the MFB @18:08:48.82 Homing MFB Syringe @18:08:48.85 AFV.select :holdingCoil @18:08:49.12 ATV.dial :air2 @18:08:49.28
SC.reconfigure SCloose @18:08:49.59 Elbow.reconfigure TightElbow @18:08:49.66 Elbow.seek 2 @18:08:49.78 AS.reconfigure ASconfig @18:08:49.85 AS.home.seek :home @18:08:51.92
Forearm.reconfigure TightForearm @18:08:51.99 Forearm.seek 2 + 700 counts @18:08:53.03 SE.reconfigure SEpick @18:08:53.15 SE.seek beamBreak @18:08:55.38 AFV.select :carrierSolution @18:08:55.51 delay 2 seconds @18:08:56.20
SE.configure SEconfig @18:08:56.41 Elbow.seek 2 @18:08:56.65 Forearm.seek 2 + 300 counts @18:08:56.95 Forearm.reconfigure LooseForearm @18:08:57.02 Hand.close @18:08:57.54 AS.seek 1ml @18:08:57.85 SE.seek :down @18:08:58.09
Forearm.seek :retract @18:08:59.09 Elbow.seek CC @18:09:01.03 SE.coast @18:09:01.27
Elbow.reconfigure LooseElbow @18:09:01.39 Forearm.reconfigure TightForearm @18:09:01.47 Forearm.seek CC @18:09:02.84 CC.seek :closed @18:09:07.09 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.57 @18:09:07.10 Hand.open @18:09:08.03 Forearm.reconfigure LooseForearm @18:09:08.10 Forearm.seek :retract @18:09:09.13 SC.coast @18:09:09.19 collectSample 1500ml w/bubble point of 55psi @18:09:09.21 Priming 7ml @18:09:09.24 Gate.power :sampler,:ON @18:09:09.26 Intake.select :close @18:09:09.29 Exhaust.select :close @18:09:09.32 SS.hold @18:09:09.59 Calibrating pressure and temperature sensors @18:09:09.61 CTV.dial :air @18:09:09.64 SSV.dial :spit,avoiding: :exhaust @18:09:09.68 SS.reconfigure SSfast @18:09:09.76 SS.seek 5ml @18:09:12.88 SSV.dial CBV @18:09:12.91
CBV.dial SSV @18:09:14.47 delay 3.8 seconds @18:09:18.41 SSV.dial :spit @18:09:19.92 SS.seek 0ml @18:09:22.57 SSV.dial CBV @18:09:24.10 CTV.dial :intake @18:09:24.98 Waiting for external sampler to finish priming... @18:09:24.99 delayUntil "18:14:09.26" @18:09:50.49 delay 1 second @18:09:51.50 AFV.select :holdingCoil @18:09:51.63 delay 1 second @18:09:52.65 ATV.dial :waste @18:09:53.74 AS.reconfigure ASnorm @18:09:53.81 AS.home.seek :empty @18:10:44.40 Session Begun @18:10:46.39 ATV.dial :waste @18:10:46.42 ARV.dial between carrier and air1 @18:10:46.55 ASV.dial :waste1 @18:10:47.01 -> pucks @18:10:52.96 -> exit @18:10:52.97 Session Ended @18:14:09.28 SSV.dial CBV @18:14:09.31
CBV.dial SSV @18:14:09.35 Allowing 40s for 7.0ml of remaining 7.0ml @18:14:09.37 Intake.select :open @18:14:11.57 Intake @25.1psia, vacuum from 18.6 to 22.6psi @18:14:11.60 SS.reconfigure SSinhale.with maxSpeed: 150 @18:14:11.68 SS.seek 7ml @18:14:17.90 Stroke completed -- waiting for pressure to equalize ... @18:14:26.73 Intake.select :close @18:14:26.91 SSV.dial :exhaust @18:14:27.78 Exhaust.select :open @18:14:27.91 SS.reconfigure SSexhale @18:14:28.00 SS.seek 0ml @18:14:32.20 Exhaust.select :close @18:14:32.35 Sampling 1500.0ml @18:14:32.38 Intake.select :close @18:14:32.40 Exhaust.select :close @18:14:32.43 SS.reconfigure SSconfig @18:14:32.52 SS.hold @18:14:32.76 CTV.dial :intake @18:14:32.78 SSV.dial CBV @18:14:32.82
CBV.dial SSV @18:14:33.71 Allowing 130s for 25.0ml of remaining 1500.0ml @18:14:33.72 Intake.select :open @18:14:35.93 Intake @26.0psia, vacuum from 19.5 to 23.5psi @18:14:35.96 SS.reconfigure SSinhale.with maxSpeed: 150 @18:14:36.05 SS.seek 25ml @18:14:40.88 Vacuum too high -- waiting up to 40s @18:14:44.46 Intake @23.6psia, vacuum from 17.1 to 21.1psi @18:14:44.49 SS.reconfigure SSinhale.with maxSpeed: 135 @18:14:44.58 SS.seek 25ml @18:14:48.40 Vacuum too high -- waiting up to 40s @18:14:53.51 Intake @24.1psia, vacuum from 17.6 to 21.6psi @18:14:53.53 SS.reconfigure SSinhale.with maxSpeed: 122 @18:14:53.62 SS.seek 25ml @18:14:58.00 Vacuum too high -- waiting up to 40s @18:15:02.09 Intake @24.3psia, vacuum from 17.8 to 21.8psi @18:15:02.11 SS.reconfigure SSinhale.with maxSpeed: 110 @18:15:02.20 SS.seek 25ml @18:15:07.35 Stroke completed -- waiting for pressure to equalize ... @18:15:12.96 Intake.select :close @18:15:13.19 SSV.dial :exhaust @18:15:14.10 Exhaust.select :open @18:15:14.24 SS.reconfigure SSexhale @18:15:14.31 SS.seek 0ml @18:15:23.57 Exhaust.select :close @18:15:23.71 SSV.dial CBV @18:15:23.74
CBV.dial SSV @18:15:24.63 Allowing 130s for 25.0ml of remaining 1475.0ml @18:15:24.64 Intake.select :open @18:15:26.85 Intake @26.3psia, vacuum from 19.8 to 23.8psi @18:15:26.87 SS.reconfigure SSinhale.with maxSpeed: 110 @18:15:26.96 SS.seek 25ml @18:15:32.44 Vacuum too high -- waiting up to 40s @18:15:34.77 Intake @23.8psia, vacuum from 17.3 to 21.3psi @18:15:34.80 SS.reconfigure SSinhale.with maxSpeed: 99 @18:15:34.88 SS.seek 25ml @18:15:40.06 Vacuum too high -- waiting up to 40s @18:15:44.18 Intake @24.3psia, vacuum from 17.8 to 21.8psi @18:15:44.20 SS.reconfigure SSinhale.with maxSpeed: 89 @18:15:44.29 SS.seek 25ml @18:15:50.31 Vacuum too high -- waiting up to 40s @18:15:54.16 Intake @24.3psia, vacuum from 17.8 to 21.8psi @18:15:54.18 SS.reconfigure SSinhale.with maxSpeed: 80 @18:15:54.27 SS.seek 25ml @18:16:00.41 Stroke completed -- waiting for pressure to equalize ... @18:16:05.38 Intake.select :close @18:16:05.56 SSV.dial :exhaust @18:16:06.42 Exhaust.select :open @18:16:06.55 SS.reconfigure SSexhale @18:16:06.63 SS.seek 0ml @18:16:16.26 Exhaust.select :close @18:16:16.39 SSV.dial CBV @18:16:16.42
CBV.dial SSV @18:16:17.32 Allowing 130s for 25.0ml of remaining 1450.0ml @18:16:17.33 Intake.select :open @18:16:19.54 Intake @26.3psia, vacuum from 19.8 to 23.8psi @18:16:19.56 SS.reconfigure SSinhale.with maxSpeed: 80 @18:16:19.66 SS.seek 25ml @18:16:27.12 Vacuum too high -- waiting up to 40s @18:16:29.49 Intake @24.0psia, vacuum from 17.5 to 21.5psi @18:16:29.52 SS.reconfigure SSinhale.with maxSpeed: 72 @18:16:29.61 SS.seek 25ml @18:16:36.87 Vacuum too high -- waiting up to 40s @18:16:40.49 Intake @24.5psia, vacuum from 18.0 to 22.0psi @18:16:40.51 SS.reconfigure SSinhale.with maxSpeed: 65 @18:16:40.60 SS.seek 25ml @18:16:42.90 -> exit @18:16:42.93 Session Ended @18:16:51.81
Vacuum too high -- waiting up to 40s @18:16:55.13 Intake @24.4psia, vacuum from 17.9 to 21.9psi @18:16:55.15 SS.reconfigure SSinhale.with maxSpeed: 59 @18:16:55.20 SS.seek 25ml @18:16:56.86 Stroke completed -- waiting for pressure to equalize ... @18:16:58.19 Intake.select :close @18:16:58.35 SSV.dial :exhaust @18:16:59.21 Exhaust.select :open @18:16:59.32 SS.reconfigure SSexhale @18:16:59.37 SS.seek 0ml @18:17:08.73 Exhaust.select :close @18:17:08.85 SSV.dial CBV @18:17:08.88
CBV.dial SSV @18:17:09.75 Allowing 130s for 25.0ml of remaining 1425.0ml @18:17:09.76 Intake.select :open @18:17:11.93 Intake @26.2psia, vacuum from 19.7 to 23.7psi @18:17:11.95 SS.reconfigure SSinhale.with maxSpeed: 59 @18:17:12.00 SS.seek 25ml @18:17:31.00 Vacuum too high -- waiting up to 40s @18:17:33.85 Intake @24.2psia, vacuum from 17.7 to 21.7psi @18:17:33.87 SS.reconfigure SSinhale.with maxSpeed: 53 @18:17:33.92 SS.seek 25ml @18:17:46.84 Stroke completed -- waiting for pressure to equalize ... @18:17:51.32 Intake.select :close @18:17:51.47 SSV.dial :exhaust @18:17:52.35 Exhaust.select :open @18:17:52.46 SS.reconfigure SSexhale @18:17:52.51 SS.seek 0ml @18:18:01.98 Exhaust.select :close @18:18:02.10 SSV.dial CBV @18:18:02.13
CBV.dial SSV @18:18:02.99 Allowing 130s for 25.0ml of remaining 1400.0ml @18:18:03.00 Intake.select :open @18:18:05.18 Intake @26.1psia, vacuum from 19.6 to 23.6psi @18:18:05.19 SS.reconfigure SSinhale.with maxSpeed: 53 @18:18:05.24 SS.seek 25ml @18:18:39.12 Stroke completed -- waiting for pressure to equalize ... @18:18:45.47 Intake.select :close @18:18:45.64 SSV.dial :exhaust @18:18:46.48 Exhaust.select :open @18:18:46.60 SS.reconfigure SSexhale @18:18:46.65 SS.seek 0ml @18:18:56.05 Exhaust.select :close @18:18:56.17 SSV.dial CBV @18:18:56.19
CBV.dial SSV @18:18:57.07 Allowing 130s for 25.0ml of remaining 1375.0ml @18:18:57.09 Intake.select :open @18:18:59.26 Intake @25.9psia, vacuum from 19.4 to 23.4psi @18:18:59.28 SS.reconfigure SSinhale.with maxSpeed: 53 @18:18:59.33 SS.seek 25ml @18:19:25.87 Vacuum too high -- waiting up to 40s @18:19:28.94 Intake @24.1psia, vacuum from 17.6 to 21.6psi @18:19:28.96 SS.reconfigure SSinhale.with maxSpeed: 48 @18:19:29.01 SS.seek 25ml @18:19:37.18 Stroke completed -- waiting for pressure to equalize ... @18:19:41.52 Intake.select :close @18:19:41.66 SSV.dial :exhaust @18:19:42.52 Exhaust.select :open @18:19:42.64 SS.reconfigure SSexhale @18:19:42.70 SS.seek 0ml @18:19:52.10 Exhaust.select :close @18:19:52.22 SSV.dial CBV @18:19:52.25
CBV.dial SSV @18:19:53.11 Allowing 130s for 25.0ml of remaining 1350.0ml @18:19:53.12 Intake.select :open @18:19:55.30 Intake @26.0psia, vacuum from 19.5 to 23.5psi @18:19:55.31 SS.reconfigure SSinhale.with maxSpeed: 48 @18:19:55.36 SS.seek 25ml @18:20:32.55 Stroke completed -- waiting for pressure to equalize ... @18:20:37.74 Intake.select :close @18:20:37.90 SSV.dial :exhaust @18:20:38.75 Exhaust.select :open @18:20:38.86 SS.reconfigure SSexhale @18:20:38.91 SS.seek 0ml @18:20:48.33 Exhaust.select :close @18:20:48.45 SSV.dial CBV @18:20:48.48
CBV.dial SSV @18:20:49.36 Allowing 130s for 25.0ml of remaining 1325.0ml @18:20:49.37 Intake.select :open @18:20:51.54 Intake @26.2psia, vacuum from 19.7 to 23.7psi @18:20:51.56 SS.reconfigure SSinhale.with maxSpeed: 48 @18:20:51.61 SS.seek 25ml @18:21:29.02 Stroke completed -- waiting for pressure to equalize ... @18:21:35.45 Intake.select :close @18:21:35.60 SSV.dial :exhaust @18:21:36.47 Exhaust.select :open @18:21:36.58 SS.reconfigure SSexhale @18:21:36.63 SS.seek 0ml @18:21:46.04 Exhaust.select :close @18:21:46.16 SSV.dial CBV @18:21:46.19
CBV.dial SSV @18:21:47.05 Allowing 130s for 25.0ml of remaining 1300.0ml Intake.select :open @18:21:49.24 Intake @26.1psia, vacuum from 19.6 to 23.6psi @18:21:49.25 SS.reconfigure SSinhale.with maxSpeed: 48 @18:21:49.30 SS.seek 25ml @18:22:16.75 Vacuum too high -- waiting up to 40s @18:22:19.35 Intake @24.3psia, vacuum from 17.8 to 21.8psi @18:22:19.36 SS.reconfigure SSinhale.with maxSpeed: 43 @18:22:19.41 SS.seek 25ml @18:22:30.93 Stroke completed -- waiting for pressure to equalize ... @18:22:34.74 Intake.select :close @18:22:34.90 SSV.dial :exhaust @18:22:35.74 Exhaust.select :open @18:22:35.86 SS.reconfigure SSexhale @18:22:36.41 SS.seek 0ml @18:22:45.47 Exhaust.select :close @18:22:45.59 SSV.dial CBV @18:22:45.62
CBV.dial SSV @18:22:46.50 Allowing 130s for 25.0ml of remaining 1275.0ml @18:22:46.51 Intake.select :open @18:22:48.69 Intake @26.1psia, vacuum from 19.6 to 23.6psi @18:22:48.70 SS.reconfigure SSinhale.with maxSpeed: 43 @18:22:48.75 SS.seek 25ml @18:23:30.77 Stroke completed -- waiting for pressure to equalize ... @18:23:35.90 Intake.select :close @18:23:36.06 SSV.dial :exhaust @18:23:36.91 Exhaust.select :open @18:23:37.02 SS.reconfigure SSexhale @18:23:37.07 SS.seek 0ml @18:23:46.54 Exhaust.select :close @18:23:46.66 SSV.dial CBV @18:23:46.71
CBV.dial SSV @18:23:47.56 Allowing 130s for 25.0ml of remaining 1250.0ml @18:23:47.57 Intake.select :open @18:23:49.74 Intake @25.9psia, vacuum from 19.4 to 23.4psi @18:23:49.76 SS.reconfigure SSinhale.with maxSpeed: 43 @18:23:49.81 SS.seek 25ml @18:24:30.85 Stroke completed -- waiting for pressure to equalize ... @18:24:36.57 Intake.select :close @18:24:36.73 SSV.dial :exhaust @18:24:37.58 Exhaust.select :open @18:24:37.70 SS.reconfigure SSexhale @18:24:37.75 SS.seek 0ml @18:24:47.18 Exhaust.select :close @18:24:47.30 SSV.dial CBV @18:24:47.33
CBV.dial SSV @18:24:48.20 Allowing 130s for 25.0ml of remaining 1225.0ml @18:24:48.21 Intake.select :open @18:24:50.38 Intake @26.0psia, vacuum from 19.5 to 23.5psi @18:24:50.40 SS.reconfigure SSinhale.with maxSpeed: 43 @18:24:50.45 SS.seek 25ml @18:25:32.04 Stroke completed -- waiting for pressure to equalize ... @18:25:38.40 Intake.select :close @18:25:38.55 SSV.dial :exhaust @18:25:39.40 Exhaust.select :open @18:25:39.52 SS.reconfigure SSexhale @18:25:39.57 SS.seek 0ml @18:25:48.54 Exhaust.select :close @18:25:48.66 SSV.dial CBV @18:25:48.69
CBV.dial SSV @18:25:49.58 Allowing 130s for 25.0ml of remaining 1200.0ml @18:25:49.59 Intake.select :open @18:25:51.76 Intake @26.0psia, vacuum from 19.5 to 23.5psi @18:25:51.78 SS.reconfigure SSinhale.with maxSpeed: 43 @18:25:51.83 SS.seek 25ml @18:26:33.97 Stroke completed -- waiting for pressure to equalize ... @18:26:41.42 Intake.select :close @18:26:41.58 SSV.dial :exhaust @18:26:42.45 Exhaust.select :open @18:26:42.56 SS.reconfigure SSexhale @18:26:42.61 SS.seek 0ml @18:26:51.50 Exhaust.select :close @18:26:51.62 SSV.dial CBV @18:26:51.65
CBV.dial SSV @18:26:52.53 Allowing 130s for 25.0ml of remaining 1175.0ml @18:26:52.54 Intake.select :open @18:26:54.73 Intake @25.9psia, vacuum from 19.4 to 23.4psi @18:26:54.75 SS.reconfigure SSinhale.with maxSpeed: 43 @18:26:54.80 SS.seek 25ml @18:27:30.90 Vacuum too high -- waiting up to 40s @18:27:34.78 Intake @24.2psia, vacuum from 17.7 to 21.7psi @18:27:34.80 SS.reconfigure SSinhale.with maxSpeed: 39 @18:27:34.85 SS.seek 25ml @18:27:41.85 Stroke completed -- waiting for pressure to equalize ... @18:27:45.69 Intake.select :close @18:27:45.84 SSV.dial :exhaust @18:27:46.69 Exhaust.select :open @18:27:46.81 SS.reconfigure SSexhale @18:27:46.87 SS.seek 0ml @18:27:56.27 Exhaust.select :close @18:27:56.39 SSV.dial CBV @18:27:56.42
CBV.dial SSV @18:27:57.30 Allowing 130s for 25.0ml of remaining 1150.0ml @18:27:57.31 Intake.select :open @18:27:59.48 Intake @26.0psia, vacuum from 19.5 to 23.5psi @18:27:59.50 SS.reconfigure SSinhale.with maxSpeed: 39 @18:27:59.55 SS.seek 25ml @18:28:44.88 Stroke completed -- waiting for pressure to equalize ... @18:28:50.65 Intake.select :close @18:28:50.81 SSV.dial :exhaust @18:28:51.65 Exhaust.select :open @18:28:52.24 SS.reconfigure SSexhale @18:28:52.29 SS.seek 0ml @18:29:01.71 Exhaust.select :close @18:29:01.83 SSV.dial CBV @18:29:01.86
CBV.dial SSV @18:29:02.72 Allowing 130s for 25.0ml of remaining 1125.0ml @18:29:02.73 Intake.select :open @18:29:04.92 Intake @25.9psia, vacuum from 19.4 to 23.4psi @18:29:04.93 SS.reconfigure SSinhale.with maxSpeed: 39 @18:29:04.98 SS.seek 25ml @18:29:50.44 Stroke completed -- waiting for pressure to equalize ... @18:29:56.24 Intake.select :close @18:29:56.39 SSV.dial :exhaust @18:29:57.24 Exhaust.select :open @18:29:57.36 SS.reconfigure SSexhale @18:29:57.42 SS.seek 0ml @18:30:06.85 Exhaust.select :close @18:30:06.97 SSV.dial CBV @18:30:07.00
CBV.dial SSV @18:30:07.89 Allowing 130s for 25.0ml of remaining 1100.0ml @18:30:07.90 Intake.select :open @18:30:10.07 Intake @26.1psia, vacuum from 19.6 to 23.6psi @18:30:10.09 SS.reconfigure SSinhale.with maxSpeed: 39 @18:30:10.14 SS.seek 25ml @18:30:50.62 Vacuum too high -- waiting up to 40s @18:30:53.45 Intake @24.2psia, vacuum from 17.7 to 21.7psi @18:30:53.47 SS.reconfigure SSinhale.with maxSpeed: 35 @18:30:53.52 SS.seek 25ml @18:30:59.88 Stroke completed -- waiting for pressure to equalize ... @18:31:03.70 Intake.select :close @18:31:03.86 SSV.dial :exhaust @18:31:04.72 Exhaust.select :open @18:31:04.83 SS.reconfigure SSexhale @18:31:04.88 SS.seek 0ml @18:31:14.63 Exhaust.select :close @18:31:14.75 SSV.dial CBV @18:31:14.78
CBV.dial SSV @18:31:15.67 Allowing 130s for 25.0ml of remaining 1075.0ml @18:31:15.68 Intake.select :open @18:31:17.86 Intake @25.7psia, vacuum from 19.2 to 23.2psi @18:31:17.87 SS.reconfigure SSinhale.with maxSpeed: 35 @18:31:17.92 SS.seek 25ml @18:32:08.19 Stroke completed -- waiting for pressure to equalize ... @18:32:13.10 Intake.select :close @18:32:13.29 SSV.dial :exhaust @18:32:14.15 Exhaust.select :open @18:32:14.26 SS.reconfigure SSexhale @18:32:14.31 SS.seek 0ml @18:32:23.55 Exhaust.select :close @18:32:23.67 SSV.dial CBV @18:32:23.72
CBV.dial SSV @18:32:24.54 Allowing 130s for 25.0ml of remaining 1050.0ml @18:32:24.55 Intake.select :open @18:32:26.73 Intake @25.9psia, vacuum from 19.4 to 23.4psi @18:32:26.74 SS.reconfigure SSinhale.with maxSpeed: 35 @18:32:26.79 SS.seek 25ml @18:33:17.58 Stroke completed -- waiting for pressure to equalize ... @18:33:23.35 Intake.select :close @18:33:23.50 SSV.dial :exhaust @18:33:24.36 Exhaust.select :open @18:33:24.47 SS.reconfigure SSexhale @18:33:24.52 SS.seek 0ml @18:33:34.05 Exhaust.select :close @18:33:34.17 SSV.dial CBV @18:33:34.20
CBV.dial SSV @18:33:35.07 Allowing 130s for 25.0ml of remaining 1025.0ml @18:33:35.08 Intake.select :open @18:33:37.25 Intake @25.7psia, vacuum from 19.2 to 23.2psi @18:33:37.26 SS.reconfigure SSinhale.with maxSpeed: 35 @18:33:37.31 SS.seek 25ml @18:34:27.89 Stroke completed -- waiting for pressure to equalize ... @18:34:34.25 Intake.select :close @18:34:34.40 SSV.dial :exhaust @18:34:35.26 Exhaust.select :open @18:34:35.37 SS.reconfigure SSexhale @18:34:35.42 SS.seek 0ml @18:34:44.66 Exhaust.select :close @18:34:44.78 SSV.dial CBV @18:34:44.81
CBV.dial SSV @18:34:45.70 Allowing 130s for 25.0ml of remaining 1000.0ml @18:34:45.71 Intake.select :open @18:34:47.88 Intake @26.0psia, vacuum from 19.5 to 23.5psi @18:34:47.90 SS.reconfigure SSinhale.with maxSpeed: 35 @18:34:47.95 SS.seek 25ml @18:35:38.77 Stroke completed -- waiting for pressure to equalize ... @18:35:45.77 Intake.select :close @18:35:45.93 SSV.dial :exhaust @18:35:46.79 Exhaust.select :open @18:35:47.37 SS.reconfigure SSexhale @18:35:47.42 SS.seek 0ml @18:35:56.74 Exhaust.select :close @18:35:56.86 SSV.dial CBV @18:35:56.89
CBV.dial SSV @18:35:57.76 Allowing 130s for 25.0ml of remaining 975.0ml @18:35:57.77 Intake.select :open @18:35:59.94 Intake @25.6psia, vacuum from 19.1 to 23.1psi @18:35:59.96 SS.reconfigure SSinhale.with maxSpeed: 35 @18:36:00.01 SS.seek 25ml @18:36:38.68 Vacuum too high -- waiting up to 40s @18:36:41.80 Intake @24.0psia, vacuum from 17.5 to 21.5psi @18:36:41.82 SS.reconfigure SSinhale.with maxSpeed: 32 @18:36:41.87 SS.seek 25ml @18:36:55.20 Stroke completed -- waiting for pressure to equalize ... @18:36:59.70 Intake.select :close @18:36:59.86 SSV.dial :exhaust @18:37:00.72 Exhaust.select :open @18:37:00.83 SS.reconfigure SSexhale @18:37:00.88 SS.seek 0ml @18:37:10.47 Exhaust.select :close @18:37:10.59 SSV.dial CBV @18:37:10.62
CBV.dial SSV @18:37:11.50 Allowing 130s for 25.0ml of remaining 950.0ml @18:37:11.51 Intake.select :open @18:37:13.68 Intake @25.9psia, vacuum from 19.4 to 23.4psi @18:37:13.70 SS.reconfigure SSinhale.with maxSpeed: 32 @18:37:13.75 SS.seek 25ml @18:38:08.66 Stroke completed -- waiting for pressure to equalize ... @18:38:13.74 Intake.select :close @18:38:13.90 SSV.dial :exhaust @18:38:14.73 Exhaust.select :open @18:38:14.85 SS.reconfigure SSexhale @18:38:14.91 SS.seek 0ml @18:38:24.34 Exhaust.select :close @18:38:24.46 SSV.dial CBV @18:38:24.49
CBV.dial SSV @18:38:25.37 Allowing 130s for 25.0ml of remaining 925.0ml @18:38:25.38 Intake.select :open @18:38:27.55 Intake @25.9psia, vacuum from 19.4 to 23.4psi @18:38:27.57 SS.reconfigure SSinhale.with maxSpeed: 32 @18:38:27.62 SS.seek 25ml @18:39:22.73 Stroke completed -- waiting for pressure to equalize ... @18:39:28.95 Intake.select :close @18:39:29.11 SSV.dial :exhaust @18:39:29.98 Exhaust.select :open @18:39:30.09 SS.reconfigure SSexhale @18:39:30.18 SS.seek 0ml @18:39:39.84 Exhaust.select :close @18:39:39.96 SSV.dial CBV @18:39:39.99
CBV.dial SSV @18:39:40.85 Allowing 130s for 25.0ml of remaining 900.0ml @18:39:40.86 Intake.select :open @18:39:43.03 Intake @25.5psia, vacuum from 19.0 to 23.0psi @18:39:43.05 SS.reconfigure SSinhale.with maxSpeed: 32 @18:39:43.10 SS.seek 25ml @18:40:32.15 Vacuum too high -- waiting up to 40s @18:40:34.76 Intake @24.6psia, vacuum from 18.1 to 22.1psi @18:40:34.78 SS.reconfigure SSinhale.with maxSpeed: 29 @18:40:34.83 SS.seek 25ml @18:40:41.98 Stroke completed -- waiting for pressure to equalize ... @18:40:45.83 Intake.select :close @18:40:45.98 SSV.dial :exhaust @18:40:46.84 Exhaust.select :open @18:40:46.95 SS.reconfigure SSexhale @18:40:47.02 SS.seek 0ml @18:40:56.39 Exhaust.select :close @18:40:56.51 SSV.dial CBV @18:40:56.54
CBV.dial SSV @18:40:57.40 Allowing 130s for 25.0ml of remaining 875.0ml @18:40:57.41 Intake.select :open @18:40:59.58 Intake @25.4psia, vacuum from 18.9 to 22.9psi @18:40:59.60 SS.reconfigure SSinhale.with maxSpeed: 29 @18:40:59.65 SS.seek 25ml @18:42:00.81 Stroke completed -- waiting for pressure to equalize ... @18:42:05.91 Intake.select :close @18:42:06.07 SSV.dial :exhaust @18:42:06.91 Exhaust.select :open @18:42:07.03 SS.reconfigure SSexhale @18:42:07.08 SS.seek 0ml @18:42:16.61 Exhaust.select :close @18:42:16.73 SSV.dial CBV @18:42:16.76
CBV.dial SSV @18:42:17.65 Allowing 130s for 25.0ml of remaining 850.0ml @18:42:17.66 Intake.select :open @18:42:19.83 Intake @25.8psia, vacuum from 19.3 to 23.3psi @18:42:19.85 SS.reconfigure SSinhale.with maxSpeed: 29 @18:42:19.90 SS.seek 25ml @18:43:20.70 Stroke completed -- waiting for pressure to equalize ... @18:43:26.31 Intake.select :close @18:43:26.47 SSV.dial :exhaust @18:43:27.32 Exhaust.select :open @18:43:27.43 SS.reconfigure SSexhale @18:43:27.48 SS.seek 0ml @18:43:36.98 Exhaust.select :close @18:43:37.10 SSV.dial CBV @18:43:37.13
CBV.dial SSV @18:43:37.99 Allowing 130s for 25.0ml of remaining 825.0ml @18:43:38.00 Intake.select :open @18:43:40.17 Intake @25.5psia, vacuum from 19.0 to 23.0psi @18:43:40.19 SS.reconfigure SSinhale.with maxSpeed: 29 @18:43:40.24 SS.seek 25ml @18:44:41.37 Stroke completed -- waiting for pressure to equalize ... @18:44:47.73 Intake.select :close @18:44:47.89 SSV.dial :exhaust @18:44:48.73 Exhaust.select :open @18:44:48.85 SS.reconfigure SSexhale @18:44:48.90 SS.seek 0ml @18:44:58.34 Exhaust.select :close @18:44:58.46 SSV.dial CBV @18:44:58.49
CBV.dial SSV @18:44:59.35 Allowing 130s for 25.0ml of remaining 800.0ml @18:44:59.36 Intake.select :open @18:45:01.53 Intake @25.7psia, vacuum from 19.2 to 23.2psi @18:45:01.55 SS.reconfigure SSinhale.with maxSpeed: 29 @18:45:01.60 SS.seek 25ml @18:46:02.59 Stroke completed -- waiting for pressure to equalize ... @18:46:08.94 Intake.select :close @18:46:09.10 SSV.dial :exhaust @18:46:09.93 Exhaust.select :open @18:46:10.05 SS.reconfigure SSexhale @18:46:10.10 SS.seek 0ml @18:46:19.59 Exhaust.select :close @18:46:19.71 SSV.dial CBV @18:46:19.74
CBV.dial SSV @18:46:20.62 Allowing 130s for 25.0ml of remaining 775.0ml @18:46:20.63 Intake.select :open @18:46:22.80 Intake @25.7psia, vacuum from 19.2 to 23.2psi @18:46:22.82 SS.reconfigure SSinhale.with maxSpeed: 29 @18:46:22.87 SS.seek 25ml @18:47:07.39 Vacuum too high -- waiting up to 40s @18:47:09.94 Intake @24.5psia, vacuum from 18.0 to 22.0psi @18:47:09.96 SS.reconfigure SSinhale.with maxSpeed: 26 @18:47:10.01 SS.seek 25ml @18:47:28.93 Stroke completed -- waiting for pressure to equalize ... @18:47:33.43 Intake.select :close @18:47:33.58 SSV.dial :exhaust @18:47:34.44 Exhaust.select :open @18:47:34.55 SS.reconfigure SSexhale @18:47:34.60 SS.seek 0ml @18:47:44.17 Exhaust.select :close @18:47:44.29 SSV.dial CBV @18:47:44.32
CBV.dial SSV @18:47:45.18 Allowing 130s for 25.0ml of remaining 750.0ml @18:47:45.19 Intake.select :open @18:47:47.36 Intake @25.6psia, vacuum from 19.1 to 23.1psi @18:47:47.38 SS.reconfigure SSinhale.with maxSpeed: 26 @18:47:47.43 SS.seek 25ml @18:48:55.51 Stroke completed -- waiting for pressure to equalize ... @18:49:00.62 Intake.select :close @18:49:00.78 SSV.dial :exhaust @18:49:01.63 Exhaust.select :open @18:49:01.74 SS.reconfigure SSexhale @18:49:01.79 SS.seek 0ml @18:49:11.20 Exhaust.select :close @18:49:11.31 SSV.dial CBV @18:49:11.34
CBV.dial SSV @18:49:12.23 Allowing 130s for 25.0ml of remaining 725.0ml @18:49:12.24 Intake.select :open @18:49:14.41 Intake @25.7psia, vacuum from 19.2 to 23.2psi @18:49:14.42 SS.reconfigure SSinhale.with maxSpeed: 26 @18:49:14.47 SS.seek 25ml @18:50:22.59 Stroke completed -- waiting for pressure to equalize ... @18:50:28.32 Intake.select :close @18:50:28.47 SSV.dial :exhaust @18:50:29.33 Exhaust.select :open @18:50:29.44 SS.reconfigure SSexhale @18:50:29.49 SS.seek 0ml @18:50:39.32 Exhaust.select :close @18:50:39.44 SSV.dial CBV @18:50:39.47
CBV.dial SSV @18:50:40.33 Allowing 130s for 25.0ml of remaining 700.0ml @18:50:40.34 Intake.select :open @18:50:42.51 Intake @25.7psia, vacuum from 19.2 to 23.2psi @18:50:42.53 SS.reconfigure SSinhale.with maxSpeed: 26 @18:50:42.58 SS.seek 25ml @18:51:50.71 Stroke completed -- waiting for pressure to equalize ... @18:51:56.30 Intake.select :close @18:51:56.46 SSV.dial :exhaust @18:51:57.32 Exhaust.select :open @18:51:57.43 SS.reconfigure SSexhale @18:51:57.48 SS.seek 0ml @18:52:07.15 Exhaust.select :close @18:52:07.27 SSV.dial CBV @18:52:07.30
CBV.dial SSV @18:52:08.18 Allowing 130s for 25.0ml of remaining 675.0ml @18:52:08.19 Intake.select :open @18:52:10.38 Intake @25.4psia, vacuum from 18.9 to 22.9psi @18:52:10.39 SS.reconfigure SSinhale.with maxSpeed: 26 @18:52:10.44 SS.seek 25ml @18:53:09.14 Vacuum too high -- waiting up to 40s @18:53:11.26 Intake @24.6psia, vacuum from 18.1 to 22.1psi @18:53:11.28 SS.reconfigure SSinhale.with maxSpeed: 23 @18:53:11.33 SS.seek 25ml @18:53:21.79 Stroke completed -- waiting for pressure to equalize ... @18:53:25.62 Intake.select :close @18:53:25.78 SSV.dial :exhaust @18:53:26.65 Exhaust.select :open @18:53:26.76 SS.reconfigure SSexhale @18:53:26.81 SS.seek 0ml @18:53:36.46 Exhaust.select :close @18:53:36.58 SSV.dial CBV @18:53:36.61
CBV.dial SSV @18:53:37.47 Allowing 130s for 25.0ml of remaining 650.0ml @18:53:37.48 Intake.select :open @18:53:39.65 Intake @25.3psia, vacuum from 18.8 to 22.8psi @18:53:39.67 SS.reconfigure SSinhale.with maxSpeed: 23 @18:53:39.72 SS.seek 25ml @18:54:56.29 Stroke completed -- waiting for pressure to equalize ... @18:55:00.74 Intake.select :close @18:55:00.89 SSV.dial :exhaust @18:55:01.76 Exhaust.select :open @18:55:01.87 SS.reconfigure SSexhale @18:55:01.92 SS.seek 0ml @18:55:11.32 Exhaust.select :close @18:55:11.44 SSV.dial CBV @18:55:11.47
CBV.dial SSV @18:55:12.35 Allowing 130s for 25.0ml of remaining 625.0ml @18:55:12.36 Intake.select :open @18:55:14.53 Intake @24.4psia, vacuum from 17.9 to 21.9psi @18:55:14.54 SS.reconfigure SSinhale.with maxSpeed: 23 @18:55:14.60 SS.seek 25ml @18:56:23.80 Vacuum too high -- waiting up to 40s @18:56:26.35 Intake @22.6psia, vacuum from 16.1 to 20.1psi @18:56:26.37 SS.reconfigure SSinhale.with maxSpeed: 21 @18:56:26.42 SS.seek 25ml @18:56:36.26 Stroke completed -- waiting for pressure to equalize ... @18:56:40.74 Intake.select :close @18:56:40.90 SSV.dial :exhaust @18:56:41.77 Exhaust.select :open @18:56:41.89 SS.reconfigure SSexhale @18:56:41.94 SS.seek 0ml @18:56:51.62 Exhaust.select :close @18:56:51.74 SSV.dial CBV @18:56:51.77
CBV.dial SSV @18:56:52.63 Allowing 130s for 25.0ml of remaining 600.0ml @18:56:52.64 Intake.select :open @18:56:54.81 Intake @23.6psia, vacuum from 17.1 to 21.1psi @18:56:54.83 SS.reconfigure SSinhale.with maxSpeed: 21 @18:56:54.88 SS.seek 25ml @18:58:19.60 Stroke completed -- waiting for pressure to equalize ... @18:58:24.70 Intake.select :close @18:58:24.85 SSV.dial :exhaust @18:58:25.71 Exhaust.select :open @18:58:25.82 SS.reconfigure SSexhale @18:58:25.87 SS.seek 0ml @18:58:35.51 Exhaust.select :close @18:58:35.63 SSV.dial CBV @18:58:35.66
CBV.dial SSV @18:58:36.53 Allowing 130s for 25.0ml of remaining 575.0ml @18:58:36.54 Intake.select :open @18:58:38.71 Intake @23.8psia, vacuum from 17.3 to 21.3psi @18:58:38.73 SS.reconfigure SSinhale.with maxSpeed: 21 @18:58:38.78 SS.seek 25ml @19:00:03.29 Stroke completed -- waiting for pressure to equalize ... @19:00:08.37 Intake.select :close @19:00:08.53 SSV.dial :exhaust @19:00:09.37 Exhaust.select :open @19:00:09.49 SS.reconfigure SSexhale @19:00:09.54 SS.seek 0ml @19:00:19.01 Exhaust.select :close @19:00:19.13 SSV.dial CBV @19:00:19.16
CBV.dial SSV @19:00:20.03 Allowing 130s for 25.0ml of remaining 550.0ml @19:00:20.04 Intake.select :open @19:00:22.22 Intake @24.4psia, vacuum from 17.9 to 21.9psi @19:00:22.23 SS.reconfigure SSinhale.with maxSpeed: 21 @19:00:22.28 SS.seek 25ml @19:01:46.87 Stroke completed -- waiting for pressure to equalize ... @19:01:52.44 Intake.select :close @19:01:52.60 SSV.dial :exhaust @19:01:53.46 Exhaust.select :open @19:01:53.57 SS.reconfigure SSexhale @19:01:53.62 SS.seek 0ml @19:02:03.34 Exhaust.select :close @19:02:03.46 SSV.dial CBV @19:02:03.49
CBV.dial SSV @19:02:04.36 Allowing 130s for 25.0ml of remaining 525.0ml @19:02:04.37 Intake.select :open @19:02:06.54 Intake @24.4psia, vacuum from 17.9 to 21.9psi @19:02:06.56 SS.reconfigure SSinhale.with maxSpeed: 21 @19:02:06.61 SS.seek 25ml @19:03:30.91 Stroke completed -- waiting for pressure to equalize ... @19:03:36.66 Intake.select :close @19:03:36.82 SSV.dial :exhaust @19:03:37.67 Exhaust.select :open @19:03:37.78 SS.reconfigure SSexhale @19:03:37.83 SS.seek 0ml @19:03:47.35 Exhaust.select :close @19:03:47.47 SSV.dial CBV @19:03:47.50
CBV.dial SSV @19:03:48.36 Allowing 130s for 25.0ml of remaining 500.0ml @19:03:48.37 Intake.select :open @19:03:50.54 Intake @24.3psia, vacuum from 17.8 to 21.8psi @19:03:50.56 SS.reconfigure SSinhale.with maxSpeed: 21 @19:03:50.61 SS.seek 25ml @19:05:15.38 Stroke completed -- waiting for pressure to equalize ... @19:05:21.10 Intake.select :close @19:05:21.26 SSV.dial :exhaust @19:05:22.10 Exhaust.select :open @19:05:22.22 SS.reconfigure SSexhale @19:05:22.27 SS.seek 0ml @19:05:31.97 Exhaust.select :close @19:05:32.09 SSV.dial CBV @19:05:32.12
CBV.dial SSV @19:05:33.00 Allowing 130s for 25.0ml of remaining 475.0ml @19:05:33.01 Intake.select :open @19:05:35.18 Intake @24.4psia, vacuum from 17.9 to 21.9psi @19:05:35.20 SS.reconfigure SSinhale.with maxSpeed: 21 @19:05:35.25 SS.seek 25ml @19:06:20.48 Vacuum too high -- waiting up to 40s @19:06:22.05 Intake @23.5psia, vacuum from 17.0 to 21.0psi @19:06:22.07 SS.reconfigure SSinhale.with maxSpeed: 19 @19:06:22.12 SS.seek 25ml @19:07:05.41 Stroke completed -- waiting for pressure to equalize ... @19:07:10.36 Intake.select :close @19:07:10.53 SSV.dial :exhaust @19:07:11.40 Exhaust.select :open @19:07:11.52 SS.reconfigure SSexhale @19:07:11.57 SS.seek 0ml @19:07:21.01 Exhaust.select :close @19:07:21.13 SSV.dial CBV @19:07:21.16
CBV.dial SSV @19:07:22.03 Allowing 130s for 25.0ml of remaining 450.0ml @19:07:22.04 Intake.select :open @19:07:24.21 Intake @24.4psia, vacuum from 17.9 to 21.9psi @19:07:24.23 SS.reconfigure SSinhale.with maxSpeed: 19 @19:07:24.28 SS.seek 25ml @19:08:57.94 Stroke completed -- waiting for pressure to equalize ... @19:09:03.05 Intake.select :close @19:09:03.21 SSV.dial :exhaust @19:09:04.05 Exhaust.select :open @19:09:04.17 SS.reconfigure SSexhale @19:09:04.22 SS.seek 0ml @19:09:13.66 Exhaust.select :close @19:09:13.78 SSV.dial CBV @19:09:13.81
CBV.dial SSV @19:09:14.67 Allowing 130s for 25.0ml of remaining 425.0ml @19:09:14.68 Intake.select :open @19:09:16.85 Intake @24.6psia, vacuum from 18.1 to 22.1psi @19:09:16.87 SS.reconfigure SSinhale.with maxSpeed: 19 @19:09:16.92 SS.seek 25ml @19:10:50.54 Stroke completed -- waiting for pressure to equalize ... @19:10:56.30 Intake.select :close @19:10:56.46 SSV.dial :exhaust @19:10:57.30 Exhaust.select :open @19:10:57.42 SS.reconfigure SSexhale @19:10:57.47 SS.seek 0ml @19:11:07.19 Exhaust.select :close @19:11:07.30 SSV.dial CBV @19:11:07.33
CBV.dial SSV @19:11:08.19 Allowing 130s for 25.0ml of remaining 400.0ml @19:11:08.20 Intake.select :open @19:11:10.38 Intake @24.6psia, vacuum from 18.1 to 22.1psi @19:11:10.39 SS.reconfigure SSinhale.with maxSpeed: 19 @19:11:10.44 SS.seek 25ml @19:12:43.83 Stroke completed -- waiting for pressure to equalize ... @19:12:48.94 Intake.select :close @19:12:49.09 SSV.dial :exhaust @19:12:49.95 Exhaust.select :open @19:12:50.06 SS.reconfigure SSexhale @19:12:50.11 SS.seek 0ml @19:12:59.63 Exhaust.select :close @19:12:59.75 SSV.dial CBV @19:13:00.36
CBV.dial SSV @19:13:01.20 Allowing 130s for 25.0ml of remaining 375.0ml @19:13:01.21 Intake.select :open @19:13:03.38 Intake @24.4psia, vacuum from 17.9 to 21.9psi @19:13:03.40 SS.reconfigure SSinhale.with maxSpeed: 19 @19:13:03.45 SS.seek 25ml @19:14:23.01 Vacuum too high -- waiting up to 40s @19:14:24.61 Intake @23.2psia, vacuum from 16.7 to 20.7psi @19:14:24.63 SS.reconfigure SSinhale.with maxSpeed: 17 @19:14:24.68 SS.seek 25ml @19:14:41.08 Stroke completed -- waiting for pressure to equalize ... @19:14:46.20 Intake.select :close @19:14:46.36 SSV.dial :exhaust @19:14:47.22 Exhaust.select :open @19:14:47.33 SS.reconfigure SSexhale @19:14:47.38 SS.seek 0ml @19:14:56.76 Exhaust.select :close @19:14:56.92 SSV.dial CBV @19:14:56.95
CBV.dial SSV @19:14:57.83 Allowing 130s for 25.0ml of remaining 350.0ml @19:14:57.84 Intake.select :open @19:15:00.26 Intake @24.5psia, vacuum from 18.0 to 22.0psi @19:15:00.27 SS.reconfigure SSinhale.with maxSpeed: 17 @19:15:00.32 SS.seek 25ml @19:16:45.47 Stroke completed -- waiting for pressure to equalize ... @19:16:50.56 Intake.select :close @19:16:50.72 SSV.dial :exhaust @19:16:51.57 Exhaust.select :open @19:16:51.68 SS.reconfigure SSexhale @19:16:51.73 SS.seek 0ml @19:17:01.14 Exhaust.select :close @19:17:01.26 SSV.dial CBV @19:17:01.29
CBV.dial SSV @19:17:02.17 Allowing 130s for 25.0ml of remaining 325.0ml @19:17:02.18 Intake.select :open @19:17:04.35 Intake @24.3psia, vacuum from 17.8 to 21.8psi @19:17:04.37 SS.reconfigure SSinhale.with maxSpeed: 17 @19:17:04.42 SS.seek 25ml @19:18:49.77 Stroke completed -- waiting for pressure to equalize ... @19:18:55.50 Intake.select :close @19:18:55.66 SSV.dial :exhaust @19:18:56.53 Exhaust.select :open @19:18:56.64 SS.reconfigure SSexhale @19:18:56.69 SS.seek 0ml @19:19:06.24 Exhaust.select :close @19:19:06.84 SSV.dial CBV @19:19:06.87
CBV.dial SSV @19:19:07.73 Allowing 130s for 25.0ml of remaining 300.0ml @19:19:07.74 Intake.select :open @19:19:09.92 Intake @24.2psia, vacuum from 17.7 to 21.7psi @19:19:09.94 SS.reconfigure SSinhale.with maxSpeed: 17 @19:19:09.99 SS.seek 25ml @19:20:54.91 Stroke completed -- waiting for pressure to equalize ... @19:21:00.67 Intake.select :close @19:21:00.83 SSV.dial :exhaust @19:21:01.68 Exhaust.select :open @19:21:01.79 SS.reconfigure SSexhale @19:21:01.84 SS.seek 0ml @19:21:11.34 Exhaust.select :close @19:21:11.46 SSV.dial CBV @19:21:11.49
CBV.dial SSV @19:21:12.35 Allowing 130s for 25.0ml of remaining 275.0ml @19:21:12.36 Intake.select :open @19:21:14.53 Intake @24.1psia, vacuum from 17.6 to 21.6psi @19:21:14.55 SS.reconfigure SSinhale.with maxSpeed: 17 @19:21:14.60 SS.seek 25ml @19:22:59.88 Stroke completed -- waiting for pressure to equalize ... @19:23:06.23 Intake.select :close @19:23:06.39 SSV.dial :exhaust @19:23:07.24 Exhaust.select :open @19:23:07.35 SS.reconfigure SSexhale @19:23:07.40 SS.seek 0ml @19:23:16.80 Exhaust.select :close @19:23:16.92 SSV.dial CBV @19:23:16.95
CBV.dial SSV @19:23:17.82 Allowing 130s for 25.0ml of remaining 250.0ml @19:23:17.83 Intake.select :open @19:23:20.00 Intake @24.1psia, vacuum from 17.6 to 21.6psi @19:23:20.02 SS.reconfigure SSinhale.with maxSpeed: 17 @19:23:20.07 SS.seek 25ml @19:25:05.62 Stroke completed -- waiting for pressure to equalize ... @19:25:13.21 Intake.select :close @19:25:13.36 SSV.dial :exhaust @19:25:14.23 Exhaust.select :open @19:25:14.34 SS.reconfigure SSexhale @19:25:14.39 SS.seek 0ml @19:25:23.75 Exhaust.select :close @19:25:23.87 SSV.dial CBV @19:25:23.90
CBV.dial SSV @19:25:24.76 Allowing 130s for 25.0ml of remaining 225.0ml @19:25:24.77 Intake.select :open @19:25:26.94 Intake @24.3psia, vacuum from 17.8 to 21.8psi @19:25:26.96 SS.reconfigure SSinhale.with maxSpeed: 17 @19:25:27.01 SS.seek 25ml @19:26:38.50 Vacuum too high -- waiting up to 40s @19:26:40.10 Intake @22.8psia, vacuum from 16.3 to 20.3psi @19:26:40.12 SS.reconfigure SSinhale.with maxSpeed: 15 @19:26:40.17 SS.seek 25ml @19:27:19.26 Stroke completed -- waiting for pressure to equalize ... @19:27:25.63 Intake.select :close @19:27:25.79 SSV.dial :exhaust @19:27:26.65 Exhaust.select :open @19:27:26.76 SS.reconfigure SSexhale @19:27:26.81 SS.seek 0ml @19:27:36.40 Exhaust.select :close @19:27:36.52 SSV.dial CBV @19:27:36.55
CBV.dial SSV @19:27:37.41 Allowing 130s for 25.0ml of remaining 200.0ml @19:27:37.42 Intake.select :open @19:27:39.59 Intake @24.1psia, vacuum from 17.6 to 21.6psi @19:27:39.61 SS.reconfigure SSinhale.with maxSpeed: 15 @19:27:39.66 SS.seek 25ml @19:29:38.72 Stroke completed -- waiting for pressure to equalize ... @19:29:45.09 Intake.select :close @19:29:45.25 SSV.dial :exhaust @19:29:46.09 Exhaust.select :open @19:29:46.21 SS.reconfigure SSexhale @19:29:46.26 SS.seek 0ml @19:29:55.59 Exhaust.select :close @19:29:55.71 SSV.dial CBV @19:29:55.74
CBV.dial SSV @19:29:56.60 Allowing 130s for 25.0ml of remaining 175.0ml @19:29:56.61 Intake.select :open @19:29:58.79 Intake @24.1psia, vacuum from 17.6 to 21.6psi @19:29:58.80 SS.reconfigure SSinhale.with maxSpeed: 15 @19:29:58.85 SS.seek 25ml @19:31:58.62 Stroke completed -- waiting for pressure to equalize ... @19:32:06.08 Intake.select :close @19:32:06.24 SSV.dial :exhaust @19:32:07.10 Exhaust.select :open @19:32:07.21 SS.reconfigure SSexhale @19:32:07.26 SS.seek 0ml @19:32:16.75 Exhaust.select :close @19:32:16.87 SSV.dial CBV @19:32:16.90
CBV.dial SSV @19:32:17.77 Allowing 130s for 25.0ml of remaining 150.0ml @19:32:17.78 Intake.select :open @19:32:19.96 Intake @24.1psia, vacuum from 17.6 to 21.6psi @19:32:19.97 SS.reconfigure SSinhale.with maxSpeed: 15 @19:32:20.02 SS.seek 25ml @19:34:20.39 Stroke completed -- waiting for pressure to equalize ... @19:34:27.40 Intake.select :close @19:34:27.55 SSV.dial :exhaust @19:34:28.41 Exhaust.select :open @19:34:28.52 SS.reconfigure SSexhale @19:34:28.57 SS.seek 0ml @19:34:38.09 Exhaust.select :close @19:34:38.21 SSV.dial CBV @19:34:38.24
CBV.dial SSV @19:34:39.12 Allowing 130s for 25.0ml of remaining 125.0ml @19:34:39.13 Intake.select :open @19:34:41.31 Intake @23.7psia, vacuum from 17.2 to 21.2psi @19:34:41.32 SS.reconfigure SSinhale.with maxSpeed: 15 @19:34:41.37 SS.seek 25ml @19:36:29.84 Vacuum too high -- waiting up to 40s @19:36:31.16 Intake @22.6psia, vacuum from 16.1 to 20.1psi @19:36:31.18 SS.reconfigure SSinhale.with maxSpeed: 14 @19:36:31.24 SS.seek 25ml @19:36:44.03 Stroke completed -- waiting for pressure to equalize ... @19:36:49.14 SS.reconfigure SSconfig @19:36:49.19 SS.hold @19:36:49.31 Intake.select :close @19:36:49.47 ShallowSampler::Clogged in MAIN -- progress too slow after 1400.1ml @19:36:49.48 SSV.dial :exhaust @19:36:50.33 Exhaust.select :open @19:36:50.44 SS.reconfigure SSexhale @19:36:50.49 SS.seek 0ml @19:36:59.92 Exhaust.select :close @19:37:00.05 Sampled 1400.1ml @19:37:00.07 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @19:37:00.08 Intake.select :close @19:37:00.14 CSV.dial :waste @19:37:00.21 CS.seek 0ml @19:37:00.31 SSV.dial CBV,avoiding: :exhaust @19:37:00.34
CBV.dial SSV @19:37:00.41 CSV.dial :air @19:37:01.38 CS.seek 10ml @19:37:02.46
CTV.dial :air @19:37:03.05 Gate.power :sampler,:OFF @19:37:03.59
delay 15 seconds @19:37:18.62 SSV.dial :spit,avoiding: :exhaust @19:37:20.10 CTV.dial CSV,avoiding: :intake @19:37:20.15
SS.seek 0ml @19:37:20.29 SSV.dial CBV,avoiding: :exhaust @19:37:28.38 CBV.dial between SSV and waste2 @19:37:28.80 CTV.dial CSV,avoiding: :air @19:37:28.83
CSV.dial CTV @19:37:29.35 CS.reconfigure CSpressurize @19:37:29.40 CS.seek 0ml @19:37:55.18 BubblePoint: Intake @66.3psia, Outlet @10.7psia @19:37:55.19 CBV.dial SSV @19:37:55.57 SS.reconfigure SSconfig @19:37:55.62 SS.seek 10ml @19:38:01.11 delay 15 seconds @19:38:16.25 Evacuated: Intake @34.4psia, Outlet @-3.2psia @19:38:16.26 SSV.dial :spit,avoiding: :exhaust @19:38:17.74 SS.reconfigure SSexhale @19:38:17.79 SS.seek 0ml @19:38:22.86 SS.reconfigure SSfast @19:38:22.91 SS.seek 5ml @19:38:25.90 SSV.dial CBV,avoiding: :exhaust @19:38:25.93
CBV.dial SSV @19:38:27.45 CTV.dial :air,avoiding: :intake @19:38:28.96 CSV.dial CTV @19:38:29.01
SS.seek 10ml @19:38:30.00 CSV.dial :air @19:38:30.51 CS.reconfigure CSfast @19:38:30.57 CS.seek 0ml @19:38:31.55
SSV.dial :spit,avoiding: :exhaust @19:38:31.87 CS.coast @19:38:33.06
SS.reconfigure SSexhale @19:38:33.11 SS.seek 0ml @19:38:38.31 SS.reconfigure SSfast @19:38:38.36 SS.seek 5ml @19:38:41.58 SSV.dial CBV,avoiding: :exhaust @19:38:41.61
CBV.dial SSV @19:38:44.14 SSV.dial :spit,avoiding: :exhaust @19:38:45.65 SS.reconfigure SSconfig @19:38:45.70 SS.seek 0ml @19:38:48.79 SS.coast @19:38:48.84 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @19:38:48.85 Intake.select :close @19:38:48.93 CSV.dial :waste @19:38:49.07 SSV.dial CBV,avoiding: :exhaust @19:38:49.10
CBV.dial SSV @19:38:49.82 CS.seek 0ml @19:38:49.89 CSV.dial :air @19:38:50.60
CTV.dial :air @19:38:50.61 delay 15 seconds @19:38:50.72 CS.seek 10ml @19:39:05.64
SSV.dial :spit,avoiding: :exhaust @19:39:07.11 CTV.dial CSV,avoiding: :intake @19:39:07.15
SS.reconfigure SSexhale @19:39:07.26 SS.seek 0ml @19:39:07.75 SSV.dial CBV,avoiding: :exhaust @19:39:17.45 CBV.dial between SSV and waste2 @19:39:17.89 CTV.dial CSV,avoiding: :air @19:39:17.92
CSV.dial CTV @19:39:18.84 CS.reconfigure CSpressurize @19:39:18.96 CS.seek 0ml @19:39:45.20 CSV.dial :air @19:39:45.71 CS.reconfigure CSfast @19:39:45.76 CS.seek 10ml @19:40:12.13 CSV.dial between air and CTV @19:40:12.50 CS.seek 3.212ml @19:40:30.73 CTV.dial CSV,avoiding: :air @19:40:30.76
CSV.dial CTV @19:40:31.10 CS.reconfigure CSpressurize @19:40:31.15 CS.seek 0ml @19:40:38.88 BubblePoint: Intake @65.4psia, Outlet @10.3psia @19:40:38.89 CBV.dial SSV @19:40:39.24 SS.reconfigure SSconfig @19:40:39.30 SS.seek 10ml @19:40:45.04 delay 15 seconds @19:41:00.19 Evacuated: Intake @39.0psia, Outlet @10.4psia @19:41:00.20 SSV.dial :spit,avoiding: :exhaust @19:41:01.69 SS.reconfigure SSexhale @19:41:01.75 SS.seek 0ml @19:41:07.44 SS.reconfigure SSfast @19:41:07.49 SS.seek 5ml @19:41:10.10 SSV.dial CBV,avoiding: :exhaust @19:41:10.12
CBV.dial SSV @19:41:11.61 CTV.dial :air,avoiding: :intake @19:41:13.10 CSV.dial CTV @19:41:13.15
SS.seek 10ml @19:41:14.14 CSV.dial :air @19:41:14.65 CS.reconfigure CSfast @19:41:14.70 CS.seek 0ml @19:41:15.92
SSV.dial :spit,avoiding: :exhaust @19:41:16.94 CS.coast @19:41:17.41
SS.reconfigure SSexhale @19:41:17.46 SS.seek 0ml @19:41:22.64 SS.reconfigure SSfast @19:41:22.70 SS.seek 5ml @19:41:25.57 SSV.dial CBV,avoiding: :exhaust @19:41:25.60
CBV.dial SSV @19:41:28.11 SSV.dial :spit,avoiding: :exhaust @19:41:29.58 SS.reconfigure SSconfig @19:41:29.63 SS.seek 0ml @19:41:32.90 SS.coast @19:41:32.95 Lysing: Adding 1.5ml lysis @19:41:32.96 CBV.dial between CSV and waste1,avoiding: :DBV @19:41:34.64 CTV.dial CSV @19:41:36.14 CSV.dial CSR @19:41:37.02 Pulling 1ml of CSR.lysis @19:41:37.03 CSR.select :lysis @19:41:37.15 CS.reconfigure CSconfig @19:41:37.19 CS.seek 1ml @19:41:40.73 delay 5 seconds @19:41:45.75 CSR.select :air @19:41:45.94 CS.seek 6ml @19:41:59.73 delay 2 seconds @19:42:01.75 CSV.dial :waste1 @19:42:03.21 CS.reconfigure CSfast @19:42:03.26 CS.seek :empty @19:42:19.31 CSV.dial CSR @19:42:20.84 Pulling 1.5ml of CSR.lysis @19:42:20.85 CSR.select :lysis @19:42:20.96 CS.reconfigure CSconfig @19:42:21.02 CS.seek 1.5ml @19:42:25.89 delay 5 seconds @19:42:30.91 CSR.select :air @19:42:31.10 CS.seek 4.5ml @19:42:39.69 delay 2 seconds @19:42:41.71 CSV.dial CTV @19:42:42.72 CS.seek 1.275ml @19:42:51.92 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @19:42:51.95 CH.seek 85C,8:00 @19:46:11.06 CH.hold 5:00,85C @19:51:11.23 Collection Puck Heater at 85C after lysing @19:51:11.24 CS.seek 0ml @19:51:15.77 CTV.dial between spare and air,avoiding: :intake @19:51:16.94 CSV.dial :air @19:51:17.44 CS.seek 5ml @19:51:31.19 CSV.dial CTV @19:51:31.70 delay 2 seconds @19:51:33.72 CSV.dial :air @19:51:34.24 delay 2 seconds @19:51:36.26 CSV.dial CTV @19:51:36.77 delay 2 seconds @19:51:38.79 CSV.dial :air @19:51:39.30 delay 2 seconds @19:51:41.32 CS.seek 0ml @19:51:55.30 Recovering Lysate into Collection syringe @19:51:55.31 CSV.dial CBV @19:51:56.45 CBV.dial CSV @19:51:56.87 CS.seek 5ml @19:52:10.57 delay 5 seconds @19:52:15.59 CTV.dial :air @19:52:16.18 CS.seek 7ml @19:52:22.49 delay 5 seconds @19:52:27.95 CSV.dial :air2 @19:52:28.51 CS.seek 10ml @19:52:37.13 CSV.dial between air and CTV,avoiding: CBV @19:52:38.44 Lysing Complete @19:52:38.52 Storing sh1 puck in Collection Clamp to tube 1 @19:52:38.72 SC.reconfigure SCtight @19:52:38.77 SC.seek 1 @19:52:40.93 SC.reconfigure SCloose @19:52:41.16 Elbow.reconfigure TightElbow @19:52:41.21 Elbow.seek 1 @19:52:43.14 Forearm.reconfigure TightForearm @19:52:43.19 Forearm.seek 1 + 700 counts @19:52:44.26 SE.reconfigure SEpick @19:52:44.33 SE.seek beamBreak @19:52:47.26 SE.configure SEconfig @19:52:47.32 SE.seek :place @19:52:47.98 Forearm.reconfigure LooseForearm @19:52:48.04 Forearm.seek :retract @19:52:48.98 Elbow.seek CC @19:52:50.70 Elbow.reconfigure LooseElbow @19:52:50.81 Forearm.seek CC @19:52:51.78 Hand.close @19:52:52.56 CC.seek :open @19:52:57.08 Forearm.seek :retract @19:52:58.05 Elbow.reconfigure TightElbow @19:52:58.10 Elbow.seek 1 @19:52:59.97 Elbow.reconfigure LimpElbow @19:53:00.02 Forearm.seek 1 @19:53:01.09 Elbow.hold @19:53:01.16 Elbow.reconfigure TightElbow @19:53:01.21 Hand.open @19:53:02.07 Forearm.force -20 @19:53:02.12 Forearm.seek 1 + 348 counts @19:53:02.44 Forearm.reconfigure TightForearm @19:53:02.49 Forearm.seek 1 + 700 counts @19:53:02.87 SE.seek :down @19:53:04.01
Forearm.reconfigure LooseForearm @19:53:04.06 Forearm.seek :retract @19:53:05.67 SE.coast @19:53:05.71
SC.coast @19:53:05.75 Tranferring lysate to PRV @19:53:05.76 CSV.dial :air @19:53:06.10 CS.seek 10ml @19:53:06.17 CSV.dial between air and CTV @19:53:06.53 AIV.dial :bypass @19:53:06.61 Beginning samplePassC @19:53:06.62 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:53:07.18 Hand.open @19:53:07.58 Elbow.seek FlushPuck::Garage @19:53:09.59 Elbow.reconfigure LooseElbow @19:53:09.70 Forearm.seek :garage @19:53:10.75 Hand.close @19:53:11.69 Forearm.seek :retract @19:53:12.76 Elbow.reconfigure TightElbow @19:53:12.81 Elbow.seek CC @19:53:15.65 Elbow.reconfigure LooseElbow @19:53:15.76 Forearm.reconfigure TightForearm @19:53:15.81 Forearm.seek CC @19:53:17.08 CC.seek :closed @19:53:21.35 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.31 @19:53:21.43 Hand.open @19:53:22.26 Forearm.reconfigure LooseForearm @19:53:22.31 Forearm.seek :retract @19:53:23.36 AWV.select :air @19:53:23.38 AIV.dial :bypass @19:53:23.39 PSV.dial PSR @19:53:24.93 Pulling 1.5ml of PSR.flush @19:53:24.94 PSR.select :flush @19:53:25.07 PS.seek 1.5ml @19:53:41.35 delay 5 seconds @19:53:46.37 PSV.dial :waste1 @19:53:46.90 PS.seek 0ml @19:54:03.22 PSV.dial PSR @19:54:03.87 PSR.select :air @19:54:04.00 PS.seek 2.5ml @19:54:30.54 PRV.dial CBV @19:54:30.58
CBV.dial PRV @19:54:31.47 PSV.dial :mixloop @19:54:32.63 CTV.dial :air,avoiding: :intake @19:54:32.64 PTV.dial :PRVmixing @19:54:33.46 PS.seek 0ml @19:55:00.12 PSV.dial :air @19:55:00.63 PS.seek 2.5ml @19:55:27.34 PSV.dial :mixloop @19:55:27.85 PS.seek 0ml @19:55:54.19 PSV.dial :air @19:55:54.72 PS.seek 2.5ml @19:56:21.28 PSV.dial :mixloop @19:56:21.79 PS.seek 0ml @19:56:48.32 Garaging FlushPuck in Collection Clamp @19:56:48.40 Elbow.reconfigure TightElbow @19:56:48.45 Elbow.seek CC @19:56:48.67 Elbow.reconfigure LooseElbow @19:56:48.78 Forearm.seek CC @19:56:49.73 Hand.close @19:56:50.44 CC.seek :open @19:56:55.31 Forearm.seek :retract @19:56:56.30 Elbow.reconfigure TightElbow @19:56:56.35 Elbow.seek :garage @19:56:59.48 Elbow.reconfigure LooseElbow @19:56:59.59 Forearm.reconfigure TightForearm @19:56:59.64 Forearm.seek :garage @19:57:00.82 Hand.open @19:57:01.64 Forearm.reconfigure LooseForearm @19:57:01.69 Forearm.seek :retract @19:57:02.72 Connecting PS to CS to transfer sample @19:57:02.73 CBV.dial AIV @19:57:02.74 AIV.dial :bypass @19:57:02.75 PSV.dial :mixloop @19:57:02.77 PTV.dial :PRVmixing @19:57:02.78 PRV.dial AIV @19:57:02.79 CTV.dial :air,avoiding: :intake @19:57:02.81 Loading lyfil puck from tube 2 into Collection Clamp @19:57:03.07 SC.reconfigure SCtight @19:57:03.12 SC.seek 2 @19:57:05.29 SC.reconfigure SCloose @19:57:05.52 Elbow.reconfigure TightElbow @19:57:05.57 Elbow.seek 2 @19:57:07.66 Forearm.reconfigure TightForearm @19:57:07.71 Forearm.seek 2 + 700 counts @19:57:08.74 SE.reconfigure SEpick @19:57:08.81 SE.seek beamBreak @19:57:11.91 SE.configure SEconfig @19:57:12.08 Elbow.seek 2 @19:57:12.29 Forearm.seek 2 + 300 counts @19:57:12.53 Forearm.reconfigure LooseForearm @19:57:12.57 Hand.close @19:57:13.34 SE.seek :down @19:57:13.58
Forearm.seek :retract @19:57:14.55 Elbow.seek CC @19:57:16.22 SE.coast @19:57:16.37
Elbow.reconfigure LooseElbow @19:57:16.48 Forearm.reconfigure TightForearm @19:57:16.53 Forearm.seek CC @19:57:17.75 CC.seek :closed @19:57:22.02 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.46 @19:57:22.03 Hand.open @19:57:22.90 Forearm.reconfigure LooseForearm @19:57:22.95 Forearm.seek :retract @19:57:23.91 SC.coast @19:57:23.95 CTV.dial CSV,avoiding: :intake @19:57:25.47 CSV.dial CTV @19:57:25.79 CS.seek 5ml @19:57:39.58 delay 1:20 @19:58:59.61 CS.seek 2.5ml @19:59:07.02 delay 40 seconds @19:59:47.05 CS.seek 0ml @19:59:54.49 delay 1:00 @20:00:54.51 PSV.dial :air @20:00:55.02 PS.seek 2.5ml @20:01:21.53 PSV.dial :mixloop @20:01:22.52 delay 3 seconds @20:01:25.58 PSV.dial :air @20:01:26.09 delay 2 seconds @20:01:28.10 PSV.dial :mixloop @20:01:28.62 delay 3 seconds @20:01:31.65 PSV.dial :waste1 @20:01:33.16 PS.seek :empty @20:01:59.72 CBV.dial :waste1 @20:02:00.26 delay 5 seconds @20:02:05.27 CBV.dial AIV @20:02:05.80 delay 10 seconds @20:02:15.82 CBV.dial :waste1 @20:02:16.35 delay 10 seconds @20:02:26.37 CBV.dial AIV @20:02:26.90 delay 10 seconds @20:02:36.93 CTV.dial CSV,avoiding: :intake @20:02:36.94 CBV.dial between AIV and waste1 @20:02:37.32 CSV.dial :air @20:02:37.85 CS.seek 10ml @20:03:04.29 CSV.dial CTV @20:03:04.80 CS.seek 0ml @20:03:31.54 CBV.dial :waste1 @20:03:31.88 delay 10 seconds @20:03:38.18 Session Begun @20:03:41.91
CTV.dial :air,avoiding: :intake @20:03:42.25 -> showlog 100 @20:03:43.52
Storing lyfil puck in Collection Clamp to tube 1 @20:03:43.73 SC.reconfigure SCtight @20:03:43.80 SC.seek 1 @20:03:45.98 SC.reconfigure SCloose @20:03:46.27 Elbow.reconfigure TightElbow @20:03:46.34 Elbow.seek 1 @20:03:48.26 Forearm.reconfigure TightForearm @20:03:48.34 Forearm.seek 1 + 700 counts @20:03:49.39 SE.reconfigure SEpick @20:03:49.48 SE.seek beamBreak @20:03:52.69 SE.configure SEconfig @20:03:52.89 Elbow.reconfigure LimpElbow @20:03:52.96 Forearm.reconfigure LooseForearm @20:03:53.03 Forearm.seek 1 @20:03:53.34 Elbow.hold @20:03:53.44 Elbow.reconfigure TightElbow @20:03:53.57 SE.seek 0.07 Pucks below Top Plate @20:03:54.07 Forearm.force -20 @20:03:54.13 Forearm.seek 1 + 350 counts @20:03:54.50 Forearm.reconfigure TightForearm @20:03:54.57 Forearm.seek 1 + 700 counts @20:03:54.88 SE.seek 1.07 Pucks below Top Plate @20:03:55.50 Forearm.reconfigure LooseForearm @20:03:55.57 Forearm.seek :retract @20:03:56.53 Elbow.seek CC @20:03:58.29 Elbow.reconfigure LooseElbow @20:03:58.43 Forearm.seek CC @20:03:59.45 Hand.close @20:04:00.20 CC.seek :open @20:04:04.75 Forearm.seek :retract @20:04:05.74 Elbow.reconfigure TightElbow @20:04:05.81 Elbow.seek 1 @20:04:07.72 Elbow.reconfigure LimpElbow @20:04:08.24 Forearm.seek 1 @20:04:09.40 Elbow.hold @20:04:09.51 Elbow.reconfigure TightElbow @20:04:09.58 Hand.open @20:04:10.45 Forearm.force -20 @20:04:10.52 Forearm.seek 1 + 343 counts @20:04:10.87 Forearm.reconfigure TightForearm @20:04:10.94 Forearm.seek 1 + 700 counts @20:04:11.28 SE.seek :down @20:04:12.44
Forearm.reconfigure LooseForearm @20:04:12.52 Forearm.seek :retract @20:04:14.03 SE.coast @20:04:14.09
SC.coast @20:04:14.14 PSV.dial :air @20:04:15.49 PS.seek 0.499ml @20:04:21.47 delay 3 seconds @20:04:24.49 PSV.dial :mixloop @20:04:25.02 PRV.dial :air @20:04:25.53 delay 3 seconds @20:04:28.55 PRV.dial CBV @20:04:29.09 CBV.dial AIV @20:04:29.63 PRV.dial between CBV and air @20:04:30.01 PRV.dial :air @20:04:30.44 PS.reconfigure PSslow @20:04:30.51 PS.seek 0.599ml @20:04:33.06 PRV.dial :waste2 @20:04:33.93 PS.reconfigure PSconfig @20:04:34.00 PS.seek :empty @20:04:40.98 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:04:41.08 Hand.open @20:04:41.45 Elbow.seek FlushPuck::Garage @20:04:43.45 Elbow.reconfigure LooseElbow @20:04:43.58 Forearm.seek :garage @20:04:44.65 Hand.close @20:04:45.58 Forearm.seek :retract @20:04:46.68 Elbow.reconfigure TightElbow @20:04:46.75 Elbow.seek CC @20:04:49.66 Elbow.reconfigure LooseElbow @20:04:49.79 Forearm.reconfigure TightForearm @20:04:49.86 Forearm.seek CC @20:04:51.24 CC.seek :closed @20:04:55.56 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.30 @20:04:55.67 Hand.open @20:04:56.52 Forearm.reconfigure LooseForearm @20:04:56.59 Forearm.seek :retract @20:04:57.66 Clearing PML for modified lysate @20:04:57.67 CTV.dial :air,avoiding: :intake @20:04:57.70 PRV.dial :waste2 @20:04:57.73 PTV.dial :PRV,avoiding: :puck @20:04:59.12 delay 2 seconds @20:05:01.14 PTV.dial :PRVmixing,avoiding: :puck @20:05:01.98 delay 2 seconds @20:05:04.00 PSV.dial :PML @20:05:04.01 delay 2 seconds @20:05:06.06 PRV.dial :air @20:05:06.92 delay 2 seconds @20:05:09.01 PS.seek 1.499ml @20:05:25.35 delay 5 seconds @20:05:30.38 PS.seek 2.5ml @20:05:38.22 -> showlog 1000 @20:05:41.50
PSV.dial :air @20:05:42.04 PS.seek 0ml @20:06:08.60 mixing lysate with speDil @20:06:08.61 PSV.dial :PML @20:06:09.15 CBV.dial :PRV @20:06:09.16 PRV.dial :air @20:06:09.20 delay 2 seconds @20:06:11.24 PRV.dial :speDil @20:06:11.79 delay 2 seconds @20:06:13.89 PS.reconfigure PSslow1 @20:06:13.96 PS.seek 0.225ml @20:06:38.10 delay 5 seconds @20:06:43.13 PRV.dial CBV @20:06:44.01 delay 2 seconds @20:06:46.10 PS.seek 0.475ml @20:07:12.04 delay 5 seconds @20:07:17.07 PRV.dial :air @20:07:17.62 delay 2 seconds @20:07:19.66 PS.seek 0.965ml @20:08:09.79 delay 10 seconds @20:08:19.81 PRV.dial CBV @20:08:20.37 CTV.dial :air,avoiding: :intake @20:08:20.38 PSV.dial :air @20:08:20.92 PS.reconfigure PSconfig @20:08:21.00 PS.seek 2.5ml @20:08:37.35 delay 3 seconds @20:08:40.36 PSV.dial PTV @20:08:41.21 PTV.dial :PRV,avoiding: :puck @20:08:42.07 PS.seek 0ml @20:09:08.38 PSV.dial :air @20:09:09.25 PS.seek 2.5ml @20:09:35.48 delay 3 seconds @20:09:38.51 PSV.dial PTV @20:09:39.37 PTV.dial :PRV,avoiding: :puck @20:09:39.40 PS.seek 0ml @20:10:05.67 Pushing modified lysate into AIV Injection Loop @20:10:05.68 CBV.dial AIV @20:10:05.72 AIV.dial :bypass @20:10:05.75 PRV.dial CBV @20:10:05.77 PSV.dial :air @20:10:06.65 PS.seek 2.5ml @20:10:33.04 delay 3 seconds @20:10:36.05 PSV.dial :PML @20:10:36.58 PTV.dial :PRVmixing,avoiding: :puck @20:10:37.50 PS.reconfigure PSslow1 @20:10:37.57 PS.seek 1.74ml @20:11:55.04 delay 5 seconds @20:12:00.07 AIV.dial :sample @20:12:00.72 AWV.select :waste @20:12:00.92 PS.reconfigure PSslow2 @20:12:00.99 PS.seek 1.22ml @20:13:49.07 delay 15 seconds @20:14:04.09 AIV.dial :bypass @20:14:04.73 PS.reconfigure PSconfig @20:14:04.81 PS.seek 0ml @20:14:18.10 PSV.dial :air @20:14:18.64 PS.seek 2.5ml @20:14:44.91 PSV.dial :PML @20:14:45.45 PS.seek 0ml @20:15:11.69 AIV.dial :coilToMFB @20:15:11.71 AWV.select :air @20:15:11.92 Extracting Nucleic Acid from AIV @20:15:11.95
Beginning samplePassD @20:15:12.50 SPE.hold 2:46:40,55C @20:15:12.59 ASV.dial :SPEdelivery @20:15:12.70
AWV.select :air @20:15:12.71 AIV.dial :bypass @20:15:12.75 PSV.dial PSR @20:15:13.06 delay 20 seconds @20:15:14.07
Pulling 1.5ml of PSR.killMFB @20:15:14.08 PSR.select :killMFB @20:15:14.25 PS.seek 1.5ml @20:15:31.20 delay 5 seconds @20:15:33.09 primeLysateINT @20:15:33.10 AWV.select :air @20:15:33.13 AIV.dial :coilToMFB @20:15:33.16 ATV.dial AIV @20:15:35.08 AS.reconfigure ASslow1 @20:15:35.16 AS.seek 0.299ml @20:15:36.22
PSV.dial :waste1 @20:15:36.76 PS.seek 0ml @20:15:53.86 PSV.dial PSR @20:15:54.43 PSR.select :air @20:15:54.58 PS.seek 2.5ml @20:16:22.42 PRV.dial CBV @20:16:22.45
CBV.dial PRV @20:16:22.49 PSV.dial :mixloop @20:16:23.84 CTV.dial :air,avoiding: :intake @20:16:23.85 PTV.dial :PRVmixing @20:16:23.91 PS.seek 0ml @20:16:35.89 delay 10 seconds @20:16:45.92 ATV.dial :air2 @20:16:46.82 AS.seek 0.329ml @20:16:51.72
PSV.dial PSR @20:16:53.01 Pulling 1.5ml of PSR.flush @20:16:53.02 PSR.select :flush @20:16:53.14 ATV.dial :waste2 @20:16:53.23
PS.seek 1.5ml @20:16:53.76 AS.reconfigure ASnorm @20:16:53.83 AS.seek :empty @20:17:10.14
delay 5 seconds @20:17:10.87 Lysate primed! @20:17:10.88 AFV.select :holdingCoil @20:17:10.91 ATV.dial :air2 @20:17:11.61 AS.reconfigure ASslow1 @20:17:11.68 AS.seek 0.069ml @20:17:15.16
PSV.dial :waste1 @20:17:15.84 PS.seek 0ml @20:17:26.09 delay 3 seconds @20:17:29.11 ATV.dial :AIV @20:17:30.04 AS.seek 0.469ml @20:17:33.03
PSV.dial PSR @20:17:33.70 PSR.select :air @20:17:33.86 PS.seek 2.5ml @20:18:01.58 PRV.dial CBV @20:18:01.61
CBV.dial PRV @20:18:01.65 PSV.dial :mixloop @20:18:03.00 CTV.dial :air,avoiding: :intake @20:18:03.01 PTV.dial :PRVmixing @20:18:03.06 PS.seek 0ml @20:18:30.35 PSV.dial PSR @20:18:31.62 Pulling 1.5ml of PSR.flush @20:18:31.63 PSR.select :flush @20:18:31.80 PS.seek 1.5ml @20:18:48.45 delay 5 seconds @20:18:50.79 delay 5 seconds @20:18:53.47
PSV.dial :waste1 @20:18:54.04 PS.seek 0ml @20:18:55.87 Solid Phase Extraction Heater at 50.2C @20:18:55.88 ATV.dial ASV @20:18:56.32 ASV.dial :SPEdelivery @20:18:56.42 AS.seek 0.041ml @20:19:10.72
PSV.dial PSR @20:19:11.28 PSR.select :air @20:19:11.43 PS.seek 2.5ml @20:19:38.39 PRV.dial CBV @20:19:38.42
CBV.dial PRV @20:19:38.46 PSV.dial :mixloop @20:19:39.78 CTV.dial :air,avoiding: :intake @20:19:39.79 PTV.dial :PRVmixing @20:19:39.84 PS.seek 0ml @20:20:07.04 PSV.dial PSR @20:20:08.28 Pulling 1.5ml of PSR.flush @20:20:08.29 PSR.select :flush @20:20:08.46 PS.seek 1.5ml @20:20:22.75 delay 10 seconds @20:20:24.89
delay 5 seconds @20:20:29.92 PSV.dial :waste1 @20:20:30.46 PS.seek 0ml @20:20:32.78 rinseLysateLineINT @20:20:32.79 AFV.select :carrierSolution @20:20:32.93 delay 2 seconds @20:20:34.96 AS.reconfigure ASconfig @20:20:35.04 AS.seek 1ml @20:20:47.04
PSV.dial PSR @20:20:47.70 PSR.select :air @20:20:47.85 PS.seek 2.5ml @20:21:14.67 PRV.dial CBV @20:21:14.70
CBV.dial PRV @20:21:14.73 PSV.dial :mixloop @20:21:15.95 CTV.dial :air,avoiding: :intake @20:21:15.96 PTV.dial :PRVmixing @20:21:16.02 PS.reconfigure PSslow @20:21:16.09 PS.seek 1.2ml @20:21:30.45 delay 1 second @20:21:31.46 AFV.select :holdingCoil @20:21:31.59 delay 1 second @20:21:32.62 ATV.dial AIV @20:21:33.18 AIV.dial :coilToMFB @20:21:33.20 AWV.select :waste @20:21:33.34 AS.reconfigure ASslow0 @20:21:33.42 AS.seek 0ml @20:21:42.92
PSV.dial :waste1 @20:21:44.45 PS.reconfigure PSconfig @20:21:44.53 PS.seek 0ml @20:21:58.04 PSV.dial :air @20:21:59.24 PS.seek 2.5ml @20:22:25.97 PSV.dial :mixloop @20:22:26.50 PS.reconfigure PSslow @20:22:26.58 PS.seek 0ml @20:23:14.30 ATV.dial :air2 @20:23:15.02 AS.reconfigure ASslow1 @20:23:15.09 AS.seek 0.05ml @20:23:17.85
PSV.dial :air @20:23:18.39 PS.reconfigure PSconfig @20:23:18.47 PS.seek 2.5ml @20:23:25.50 ATV.dial :AIV @20:23:26.38 AS.seek 0.01ml @20:23:34.75 ATV.dial :waste @20:23:36.42 AS.seek :empty @20:23:38.89 ethanol wash @20:23:38.90 ATV.dial :air2 @20:23:40.22 AS.reconfigure ASslow2 @20:23:40.29 AS.seek 0.009ml @20:23:45.07
PSV.dial :mixloop @20:23:45.61 PS.reconfigure PSslow @20:23:45.69 PS.seek 0ml @20:23:50.70 delay 3 seconds @20:23:53.73 Pulling 0.4ml of Ethanol Wash @20:23:53.74 ATV.dial :ethanol @20:23:54.61 AS.reconfigure ASslow1 @20:23:54.68 AS.seek 0.409ml @20:24:37.05
Cleaning Collection Syringe @20:24:37.06 CSV.dial CSR @20:24:37.66 Pulling 4ml of CSR.flush @20:24:37.67 CSR.select :flush @20:24:37.83 CS.seek 4ml @20:24:49.07 delay 5 seconds @20:24:54.09 CSR.select :air @20:24:54.25 CS.seek 10ml @20:25:10.69 CSV.dial CTV @20:25:10.72
CTV.dial CSV @20:25:12.33 CBV.dial :waste1 @20:25:12.88 CS.seek 0ml @20:25:15.46 delay 3 seconds @20:25:18.48 ATV.dial :air1 @20:25:18.90 delay 3 seconds @20:25:21.99 AS.seek 0.434ml @20:25:27.31 delay 3 seconds @20:25:30.34 ATV.dial :ethanol @20:25:30.97 AS.seek 0.419ml @20:25:34.29 delay 3 seconds @20:25:37.77 ATV.dial ASV @20:25:39.21 AS.seek 0.092ml @20:25:39.42
CSV.dial CSR @20:25:40.02 Pulling 4ml of CSR.flush @20:25:40.03 CSR.select :flush @20:25:40.19 CS.seek 4ml @20:25:51.41 delay 5 seconds @20:25:56.43 CSR.select :air @20:25:56.60 CS.seek 10ml @20:26:13.00 CSV.dial CTV @20:26:13.03
CTV.dial CSV @20:26:13.58 CBV.dial :waste1 @20:26:13.62 CS.seek 0ml @20:26:40.32 Garaging FlushPuck in Collection Clamp @20:26:40.42 Elbow.reconfigure TightElbow @20:26:40.50 Elbow.seek CC @20:26:40.78 Elbow.reconfigure LooseElbow @20:26:40.91 Forearm.seek CC @20:26:41.93 Hand.close @20:26:42.72 CC.seek :open @20:26:45.29 delay 3 seconds @20:26:47.17
Forearm.seek :retract @20:26:48.19 Elbow.reconfigure TightElbow @20:26:48.29 Elbow.seek :garage @20:26:48.42 ATV.dial :waste2 @20:26:49.20 AS.reconfigure ASconfig @20:26:49.27 AS.seek :empty @20:26:51.45
Elbow.reconfigure LooseElbow @20:26:51.58 Forearm.reconfigure TightForearm @20:26:51.67 Forearm.seek :garage @20:26:52.86 Hand.open @20:26:53.67 Forearm.reconfigure LooseForearm @20:26:53.75 Forearm.seek :retract @20:26:54.83 Whole Cell Archive sampling 1500ml @20:26:54.85 Loading wcr puck from tube 2 into Collection Clamp @20:26:54.97 ATV.dial :air2 @20:26:55.23 SC.reconfigure SCtight @20:26:55.31 SC.seek 2 @20:26:55.45 AS.reconfigure ASslow1 @20:26:55.53 AS.seek 1ml @20:26:57.50 SC.reconfigure SCloose @20:26:57.77 Elbow.reconfigure TightElbow @20:26:57.85 Elbow.seek 2 @20:27:00.10 Forearm.reconfigure TightForearm @20:27:00.17 Forearm.seek 2 + 700 counts @20:27:01.25 SE.reconfigure SEpick @20:27:01.36 SE.seek beamBreak @20:27:04.51 SE.configure SEconfig @20:27:04.71 Elbow.seek 2 @20:27:05.00 Forearm.seek 2 + 300 counts @20:27:05.29 Forearm.reconfigure LooseForearm @20:27:05.36 Hand.close @20:27:06.12 SE.seek :down @20:27:06.37 Forearm.seek :retract @20:27:07.89 Elbow.seek CC @20:27:09.09 SE.coast @20:27:09.75 Elbow.reconfigure LooseElbow @20:27:09.89 Forearm.reconfigure TightForearm @20:27:09.97 Forearm.seek CC @20:27:11.33 CC.seek :closed @20:27:15.69 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.52 @20:27:15.70 Hand.open @20:27:16.54 Forearm.reconfigure LooseForearm @20:27:16.61 Forearm.seek :retract @20:27:17.66 SC.coast @20:27:17.72 collectSample 1500ml w/bubble point of 55psi @20:27:17.74 Priming 7ml @20:27:17.77 Gate.power :sampler,:ON @20:27:17.79 Intake.select :close @20:27:17.82 Exhaust.select :close @20:27:17.86 SS.hold @20:27:18.13 Calibrating pressure sensors @20:27:18.14 CTV.dial :air @20:27:19.70 SSV.dial :spit,avoiding: :exhaust @20:27:19.73 SS.reconfigure SSfast @20:27:19.82 SS.seek 5ml @20:27:22.85 SSV.dial CBV @20:27:22.88 CBV.dial SSV @20:27:24.49 delay 3.8 seconds @20:27:28.43 SSV.dial :spit @20:27:29.94 SS.seek 0ml @20:27:32.56 SSV.dial CBV @20:27:34.10 CTV.dial :intake @20:27:34.97 Waiting for external sampler to finish priming... @20:27:34.98 delayUntil "20:32:17.78" @20:30:17.20 delay 5 seconds @20:30:22.22 ATV.dial ASV @20:30:23.22 ASV.dial :SPEdelivery @20:30:23.25 AS.seek 0.02ml @20:32:17.80 SSV.dial CBV @20:32:17.83 CBV.dial SSV @20:32:17.87 Allowing 40s for 7.0ml of remaining 7.0ml @20:32:17.90 Intake.select :open @20:32:20.10 Intake @25.0psia, vacuum from 18.5 to 22.5psi @20:32:20.13 SS.reconfigure SSinhale.with maxSpeed: 150 @20:32:20.22 SS.seek 7ml @20:32:26.33 Stroke completed -- waiting for pressure to equalize ... @20:32:36.49 Intake.select :close @20:32:37.18 SSV.dial :exhaust @20:32:38.08 Exhaust.select :open @20:32:38.22 SS.reconfigure SSexhale @20:32:38.30 SS.seek 0ml @20:32:42.40 Exhaust.select :close @20:32:42.56 Sampling 1500.0ml @20:32:42.60 Intake.select :close @20:32:42.61 Exhaust.select :close @20:32:42.64 SS.reconfigure SSconfig @20:32:42.73 SS.hold @20:32:42.98 CTV.dial :intake @20:32:43.00 SSV.dial CBV @20:32:43.07 CBV.dial SSV @20:32:43.92 Allowing 130s for 25.0ml of remaining 1500.0ml @20:32:43.94 Intake.select :open @20:32:46.16 Intake @25.4psia, vacuum from 18.9 to 22.9psi @20:32:46.18 SS.reconfigure SSinhale.with maxSpeed: 150 @20:32:46.27 SS.seek 25ml @20:33:00.18 Stroke completed -- waiting for pressure to equalize ... @20:33:18.02 Intake.select :close @20:33:18.20 SSV.dial :exhaust @20:33:19.09 Exhaust.select :open @20:33:19.22 SS.reconfigure SSexhale @20:33:19.29 SS.seek 0ml @20:33:28.77 Exhaust.select :close @20:33:28.92 SSV.dial CBV @20:33:28.95 CBV.dial SSV @20:33:29.83 Allowing 130s for 25.0ml of remaining 1475.0ml @20:33:29.86 Intake.select :open @20:33:32.06 Intake @24.8psia, vacuum from 18.3 to 22.3psi @20:33:32.09 SS.reconfigure SSinhale.with maxSpeed: 150 @20:33:32.18 SS.seek 25ml @20:33:40.57 delay 5 seconds @20:33:45.59 ATV.dial :air2 @20:33:46.72 AS.seek 0.52ml @20:33:46.80 Stroke completed -- waiting for pressure to equalize ... @20:34:03.86 Intake.select :close @20:34:04.04 SSV.dial :exhaust @20:34:04.91 Exhaust.select :open @20:34:05.05 SS.reconfigure SSexhale @20:34:05.13 SS.seek 0ml @20:34:14.54 Exhaust.select :close @20:34:14.69 SSV.dial CBV @20:34:14.72 CBV.dial SSV @20:34:15.63 Allowing 130s for 25.0ml of remaining 1450.0ml @20:34:15.66 Intake.select :open @20:34:17.86 Intake @25.0psia, vacuum from 18.5 to 22.5psi @20:34:17.89 SS.reconfigure SSinhale.with maxSpeed: 150 @20:34:17.97 SS.seek 25ml @20:34:31.82 Vacuum too high -- waiting up to 40s @20:34:32.68 Intake @21.6psia, vacuum from 15.1 to 19.1psi @20:34:32.71 SS.reconfigure SSinhale.with maxSpeed: 135 @20:34:32.79 SS.seek 25ml @20:34:32.89 Stroke completed -- waiting for pressure to equalize ... @20:34:49.27 Intake.select :close @20:34:49.45 SSV.dial :exhaust @20:34:50.33 Exhaust.select :open @20:34:50.49 SS.reconfigure SSexhale @20:34:50.56 SS.seek 0ml @20:34:59.66 Exhaust.select :close @20:34:59.81 SSV.dial CBV @20:34:59.84 CBV.dial SSV @20:35:00.73 Allowing 130s for 25.0ml of remaining 1425.0ml @20:35:00.75 Intake.select :open @20:35:02.96 Intake @24.8psia, vacuum from 18.3 to 22.3psi @20:35:02.99 SS.reconfigure SSinhale.with maxSpeed: 135 @20:35:03.08 SS.seek 25ml @20:35:15.14 Vacuum too high -- waiting up to 40s @20:35:19.05 Intake @21.7psia, vacuum from 15.2 to 19.2psi @20:35:19.08 SS.reconfigure SSinhale.with maxSpeed: 122 @20:35:19.17 SS.seek 25ml @20:35:23.82 Stroke completed -- waiting for pressure to equalize ... @20:35:27.64 delay 5 seconds @20:35:32.72 ATV.dial ASV @20:35:33.77 ASV.dial :wasteR @20:35:34.17 AS.seek 0.3ml @20:35:35.94 Intake.select :close @20:35:36.13 SSV.dial :exhaust @20:35:37.01 Exhaust.select :open @20:35:37.15 SS.reconfigure SSexhale @20:35:37.23 SS.seek 0ml @20:35:46.63 Exhaust.select :close @20:35:46.78 SSV.dial CBV @20:35:46.83 CBV.dial SSV @20:35:48.22 Allowing 130s for 25.0ml of remaining 1400.0ml @20:35:48.24 Intake.select :open @20:35:50.45 Intake @24.9psia, vacuum from 18.4 to 22.4psi @20:35:50.48 SS.reconfigure SSinhale.with maxSpeed: 122 @20:35:50.57 SS.seek 25ml @20:35:59.89 Vacuum too high -- waiting up to 40s @20:36:03.91 Intake @21.9psia, vacuum from 15.4 to 19.4psi @20:36:03.94 SS.reconfigure SSinhale.with maxSpeed: 110 @20:36:04.03 SS.seek 25ml @20:36:11.14 Vacuum too high -- waiting up to 40s @20:36:18.76 delay 20 seconds @20:36:19.18 Intake @22.1psia, vacuum from 15.6 to 19.6psi @20:36:19.21 SS.reconfigure SSinhale.with maxSpeed: 99 @20:36:19.30 SS.seek 25ml @20:36:21.72 Stroke completed -- waiting for pressure to equalize ... @20:36:24.87 Intake.select :close @20:36:25.05 SSV.dial :exhaust @20:36:25.94 Exhaust.select :open @20:36:26.07 SS.reconfigure SSexhale @20:36:26.15 SS.seek 0ml @20:36:35.24 Exhaust.select :close @20:36:35.39 SSV.dial CBV @20:36:35.42 CBV.dial SSV @20:36:36.33 Allowing 130s for 25.0ml of remaining 1375.0ml @20:36:36.36 Intake.select :open @20:36:38.56 Intake @25.0psia, vacuum from 18.5 to 22.5psi @20:36:38.59 SS.reconfigure SSinhale.with maxSpeed: 99 @20:36:38.68 SS.seek 25ml @20:36:38.80 AS.seek 0.07ml @20:36:49.07 Vacuum too high -- waiting up to 40s @20:36:51.99 Intake @22.1psia, vacuum from 15.6 to 19.6psi @20:36:52.02 SS.reconfigure SSinhale.with maxSpeed: 89 @20:36:52.11 SS.seek 25ml @20:37:02.42 Vacuum too high -- waiting up to 40s @20:37:10.17 Intake @22.5psia, vacuum from 16.0 to 20.0psi @20:37:10.20 SS.reconfigure SSinhale.with maxSpeed: 80 @20:37:10.29 SS.seek 25ml @20:37:11.60 Stroke completed -- waiting for pressure to equalize ... @20:37:14.16 Intake.select :close @20:37:14.35 SSV.dial :exhaust @20:37:15.23 Exhaust.select :open @20:37:15.37 SS.reconfigure SSexhale @20:37:15.45 SS.seek 0ml @20:37:25.21 Exhaust.select :close @20:37:25.34 delay 20 seconds @20:37:25.38 SSV.dial CBV @20:37:25.41 CBV.dial SSV @20:37:26.31 Allowing 130s for 25.0ml of remaining 1350.0ml @20:37:26.34 Intake.select :open @20:37:28.54 Intake @25.0psia, vacuum from 18.5 to 22.5psi @20:37:28.57 SS.reconfigure SSinhale.with maxSpeed: 80 @20:37:28.65 SS.seek 25ml @20:37:39.92 Vacuum too high -- waiting up to 40s @20:37:41.79 Intake @22.0psia, vacuum from 15.5 to 19.5psi @20:37:41.82 SS.reconfigure SSinhale.with maxSpeed: 72 @20:37:41.90 SS.seek 25ml @20:37:45.36 ATV.dial :waste2 @20:37:46.15 AS.reconfigure ASconfig @20:37:46.22 AS.seek :empty @20:37:50.63 Elution @20:37:50.64 ATV.dial :air1 @20:37:51.83 AS.reconfigure ASslow1 @20:37:51.90 AS.seek 0.049ml @20:37:55.64 Stroke completed -- waiting for pressure to equalize ... @20:38:02.31 delay 3 seconds @20:38:03.92 Intake.select :close @20:38:04.11 SSV.dial :exhaust @20:38:05.00 Exhaust.select :open @20:38:05.14 SS.reconfigure SSexhale @20:38:05.21 SS.seek 0ml @20:38:05.33 ATV.dial :ElutionWater @20:38:06.14 AS.seek 0.109ml @20:38:14.85 Exhaust.select :close @20:38:15.00 SSV.dial CBV @20:38:15.03 CBV.dial SSV @20:38:15.94 Allowing 130s for 25.0ml of remaining 1325.0ml @20:38:15.95 Intake.select :open @20:38:18.16 Intake @25.0psia, vacuum from 18.5 to 22.5psi @20:38:18.19 SS.reconfigure SSinhale.with maxSpeed: 72 @20:38:18.28 SS.seek 25ml @20:38:18.47 delay 3 seconds @20:38:21.50 ATV.dial ASV,via: :air2 @20:38:23.68 ASV.dial :SPEelution @20:38:24.31 AFV.select :carrierSolution @20:38:24.48 AS.reconfigure ASnorm @20:38:24.56 AS.seek 0.759ml @20:38:30.17 Vacuum too high -- waiting up to 40s @20:38:30.76 Intake @22.2psia, vacuum from 15.7 to 19.7psi @20:38:30.78 SS.reconfigure SSinhale.with maxSpeed: 65 @20:38:31.43 SS.seek 25ml @20:38:39.76 Vacuum too high -- waiting up to 40s @20:38:43.95 Intake @22.3psia, vacuum from 15.8 to 19.8psi @20:38:43.97 SS.reconfigure SSinhale.with maxSpeed: 59 @20:38:44.07 SS.seek 25ml @20:38:51.63 Stroke completed -- waiting for pressure to equalize ... @20:38:55.49 Intake.select :close @20:38:55.66 SSV.dial :exhaust @20:38:56.55 Exhaust.select :open @20:38:56.69 SS.reconfigure SSexhale @20:38:56.83 SS.seek 0ml @20:38:57.63 delay 3 seconds @20:39:00.65 AFV.select :holdingCoil @20:39:00.83 AS.reconfigure ASslow1 @20:39:00.90 AS.seek 0.474ml @20:39:06.36 Exhaust.select :close @20:39:06.51 SSV.dial CBV @20:39:06.54 CBV.dial SSV @20:39:07.42 Allowing 130s for 25.0ml of remaining 1300.0ml @20:39:07.45 Intake.select :open @20:39:09.66 Intake @25.2psia, vacuum from 18.7 to 22.7psi @20:39:09.68 SS.reconfigure SSinhale.with maxSpeed: 59 @20:39:09.77 SS.seek 25ml @20:39:40.41 Stroke completed -- waiting for pressure to equalize ... @20:39:48.67 Intake.select :close @20:39:48.86 SSV.dial :exhaust @20:39:49.74 Exhaust.select :open @20:39:49.87 SS.reconfigure SSexhale @20:39:49.95 SS.seek 0ml @20:39:58.53 delay 5 seconds @20:39:59.46 Exhaust.select :close @20:39:59.60 SSV.dial CBV @20:39:59.63 CBV.dial SSV @20:40:00.55 Allowing 130s for 25.0ml of remaining 1275.0ml @20:40:00.57 Intake.select :open @20:40:03.32 Intake @25.3psia, vacuum from 18.8 to 22.8psi @20:40:03.35 SS.reconfigure SSinhale.with maxSpeed: 59 @20:40:03.44 SS.seek 25ml @20:40:03.62 AS.reconfigure ASslow2 @20:40:03.69 AS.seek 0.439ml @20:40:18.32 Vacuum too high -- waiting up to 40s @20:40:19.17 Intake @22.3psia, vacuum from 15.8 to 19.8psi @20:40:19.20 SS.reconfigure SSinhale.with maxSpeed: 53 @20:40:19.29 SS.seek 25ml @20:40:36.94 Stroke completed -- waiting for pressure to equalize ... @20:40:39.27 delay 1:00 @20:40:43.33 Intake.select :close @20:40:43.50 SSV.dial :exhaust @20:40:44.38 Exhaust.select :open @20:40:44.53 SS.reconfigure SSexhale @20:40:44.61 SS.seek 0ml @20:40:54.10 Exhaust.select :close @20:40:54.25 SSV.dial CBV @20:40:54.28 CBV.dial SSV @20:40:55.17 Allowing 130s for 25.0ml of remaining 1250.0ml @20:40:55.20 Intake.select :open @20:40:57.41 Intake @25.2psia, vacuum from 18.7 to 22.7psi @20:40:57.43 SS.reconfigure SSinhale.with maxSpeed: 53 @20:40:57.52 SS.seek 25ml @20:41:31.39 Stroke completed -- waiting for pressure to equalize ... @20:41:39.03 Intake.select :close @20:41:39.22 SSV.dial :exhaust @20:41:39.29 pushing eluate to holding coil @20:41:39.39 AS.seek 0.279ml @20:41:40.11 Exhaust.select :open @20:41:40.25 SS.reconfigure SSexhale @20:41:40.33 SS.seek 0ml @20:41:50.10 Exhaust.select :close @20:41:50.25 SSV.dial CBV @20:41:50.28 CBV.dial SSV @20:41:51.19 Allowing 130s for 25.0ml of remaining 1225.0ml @20:41:51.22 Intake.select :open @20:41:53.42 Intake @25.1psia, vacuum from 18.6 to 22.6psi @20:41:53.45 SS.reconfigure SSinhale.with maxSpeed: 53 @20:41:53.54 SS.seek 25ml @20:42:13.23 Vacuum too high -- waiting up to 40s @20:42:15.41 Intake @22.6psia, vacuum from 16.1 to 20.1psi @20:42:15.44 SS.reconfigure SSinhale.with maxSpeed: 48 @20:42:15.52 SS.seek 25ml @20:42:31.03 Stroke completed -- waiting for pressure to equalize ... @20:42:36.15 Intake.select :close @20:42:36.34 SSV.dial :exhaust @20:42:37.22 Exhaust.select :open @20:42:37.36 SS.reconfigure SSexhale @20:42:37.44 SS.seek 0ml @20:42:47.08 Exhaust.select :close @20:42:47.23 SSV.dial CBV @20:42:47.26 CBV.dial SSV @20:42:48.15 Allowing 130s for 25.0ml of remaining 1200.0ml @20:42:48.17 Intake.select :open @20:42:50.39 Intake @25.1psia, vacuum from 18.6 to 22.6psi @20:42:50.42 SS.reconfigure SSinhale.with maxSpeed: 48 @20:42:50.51 SS.seek 25ml @20:43:19.85 Vacuum too high -- waiting up to 40s @20:43:21.99 Intake @22.7psia, vacuum from 16.2 to 20.2psi @20:43:22.02 SS.reconfigure SSinhale.with maxSpeed: 43 @20:43:22.11 SS.seek 25ml @20:43:31.07 Stroke completed -- waiting for pressure to equalize ... @20:43:35.55 Intake.select :close @20:43:35.73 SSV.dial :exhaust @20:43:36.62 Exhaust.select :open @20:43:36.75 SS.reconfigure SSexhale @20:43:36.82 SS.seek 0ml @20:43:46.58 Exhaust.select :close @20:43:46.73 SSV.dial CBV @20:43:46.76 CBV.dial SSV @20:43:47.65 Allowing 130s for 25.0ml of remaining 1175.0ml @20:43:47.68 Intake.select :open @20:43:49.87 Intake @25.1psia, vacuum from 18.6 to 22.6psi @20:43:49.89 SS.reconfigure SSinhale.with maxSpeed: 43 @20:43:49.98 SS.seek 25ml @20:44:20.54 delay 30 seconds @20:44:31.07 Stroke completed -- waiting for pressure to equalize ... @20:44:36.81 Intake.select :close @20:44:37.55 SSV.dial :exhaust @20:44:38.44 Exhaust.select :open @20:44:38.59 SS.reconfigure SSexhale @20:44:38.66 SS.seek 0ml @20:44:48.27 Exhaust.select :close @20:44:48.41 SSV.dial CBV @20:44:48.44 CBV.dial SSV @20:44:49.34 Allowing 130s for 25.0ml of remaining 1150.0ml @20:44:49.36 Intake.select :open @20:44:50.57 ATV.dial :waste,via: :air3 @20:44:51.57 Intake @25.1psia, vacuum from 18.6 to 22.6psi @20:44:51.61 SS.reconfigure SSinhale.with maxSpeed: 43 @20:44:51.70 SS.seek 25ml @20:44:52.35 AS.reconfigure ASconfig @20:44:52.42 AS.seek :empty @20:45:08.83 Parking Bleach in LysateSource line and SPEcolumn @20:45:08.85 ATV.dial :air3 @20:45:09.74 AS.reconfigure ASslow1 @20:45:09.82 AS.seek 0.029ml @20:45:16.22 ATV.dial :ElutionWater @20:45:16.88 AS.seek 0.059ml @20:45:23.22 ATV.dial :air3 @20:45:23.86 AS.seek 0.069ml @20:45:26.26 delay 2 seconds @20:45:28.28 ATV.dial :bleach @20:45:28.78 AS.seek 0.769ml @20:45:32.96 Stroke completed -- waiting for pressure to equalize ... @20:45:39.38 Intake.select :close @20:45:39.55 SSV.dial :exhaust @20:45:40.45 Exhaust.select :open @20:45:40.59 SS.reconfigure SSexhale @20:45:40.67 SS.seek 0ml @20:45:50.31 Exhaust.select :close @20:45:50.46 SSV.dial CBV @20:45:50.49 CBV.dial SSV @20:45:51.39 Allowing 130s for 25.0ml of remaining 1125.0ml @20:45:51.42 Intake.select :open @20:45:53.63 Intake @25.1psia, vacuum from 18.6 to 22.6psi @20:45:53.65 SS.reconfigure SSinhale.with maxSpeed: 43 @20:45:53.75 SS.seek 25ml @20:46:22.23 Vacuum too high -- waiting up to 40s @20:46:24.09 Intake @22.9psia, vacuum from 16.4 to 20.4psi @20:46:24.12 SS.reconfigure SSinhale.with maxSpeed: 39 @20:46:24.21 SS.seek 25ml @20:46:38.58 Stroke completed -- waiting for pressure to equalize ... @20:46:43.76 Intake.select :close @20:46:44.50 SSV.dial :exhaust @20:46:45.36 Exhaust.select :open @20:46:45.50 SS.reconfigure SSexhale @20:46:45.58 SS.seek 0ml @20:46:55.08 Exhaust.select :close @20:46:55.22 SSV.dial CBV @20:46:55.25 CBV.dial SSV @20:46:56.15 Allowing 130s for 25.0ml of remaining 1100.0ml @20:46:56.17 Intake.select :open @20:46:58.38 Intake @25.1psia, vacuum from 18.6 to 22.6psi @20:46:58.40 SS.reconfigure SSinhale.with maxSpeed: 39 @20:46:58.49 SS.seek 25ml @20:47:43.99 Stroke completed -- waiting for pressure to equalize ... @20:47:49.83 delay 3 seconds @20:47:51.02 Intake.select :close @20:47:51.20 SSV.dial :exhaust @20:47:52.10 Exhaust.select :open @20:47:52.23 SS.reconfigure SSexhale @20:47:52.31 SS.seek 0ml @20:47:52.85 ATV.dial :air3 @20:47:53.43 delay 3 seconds @20:47:56.52 AS.seek 0.794ml @20:48:01.84 delay 3 seconds @20:48:02.24 Exhaust.select :close @20:48:02.38 SSV.dial CBV @20:48:02.41 CBV.dial SSV @20:48:03.31 Allowing 130s for 25.0ml of remaining 1075.0ml @20:48:03.34 Intake.select :open @20:48:04.87 ATV.dial :bleach @20:48:05.50 AS.seek 0.779ml @20:48:05.64 Intake @25.1psia, vacuum from 18.6 to 22.6psi @20:48:05.67 SS.reconfigure SSinhale.with maxSpeed: 39 @20:48:05.75 SS.seek 25ml @20:48:08.85 delay 3 seconds @20:48:11.87 ATV.dial :AIV @20:48:13.18 AS.reconfigure ASconfig @20:48:13.26 AS.seek 0.015ml @20:48:31.38 Vacuum too high -- waiting up to 40s @20:48:33.01 Intake @22.9psia, vacuum from 16.4 to 20.4psi @20:48:33.04 SS.reconfigure SSinhale.with maxSpeed: 35 @20:48:33.13 SS.seek 25ml @20:48:55.81 Stroke completed -- waiting for pressure to equalize ... @20:48:57.47 ATV.dial :waste @20:48:59.04 AS.seek :empty @20:49:00.30 ATV.dial :air3 @20:49:01.00 Intake.select :close @20:49:01.14 AS.reconfigure ASslow1 @20:49:01.78 AS.seek 0.009ml @20:49:01.90 SSV.dial :exhaust @20:49:02.81 Exhaust.select :open @20:49:02.95 SS.reconfigure SSexhale @20:49:03.03 SS.seek 0ml @20:49:04.15 delay 2 seconds @20:49:06.16 ATV.dial :bleach @20:49:06.67 AS.seek 0.579ml @20:49:12.50 Exhaust.select :close @20:49:12.65 SSV.dial CBV @20:49:12.68 CBV.dial SSV @20:49:13.59 Allowing 130s for 25.0ml of remaining 1050.0ml @20:49:13.62 Intake.select :open @20:49:15.83 Intake @25.2psia, vacuum from 18.7 to 22.7psi @20:49:15.85 SS.reconfigure SSinhale.with maxSpeed: 35 @20:49:15.94 SS.seek 25ml @20:50:06.94 Stroke completed -- waiting for pressure to equalize ... @20:50:13.35 Intake.select :close @20:50:13.53 SSV.dial :exhaust @20:50:14.40 Exhaust.select :open @20:50:14.54 SS.reconfigure SSexhale @20:50:14.62 SS.seek 0ml @20:50:24.36 Exhaust.select :close @20:50:24.51 SSV.dial CBV @20:50:24.54 CBV.dial SSV @20:50:25.45 Allowing 130s for 25.0ml of remaining 1025.0ml @20:50:25.46 Intake.select :open @20:50:27.68 Intake @25.0psia, vacuum from 18.5 to 22.5psi @20:50:27.70 SS.reconfigure SSinhale.with maxSpeed: 35 @20:50:27.79 SS.seek 25ml @20:51:01.59 delay 3 seconds @20:51:04.61 ATV.dial :air3 @20:51:05.20 delay 3 seconds @20:51:08.28 AS.seek 0.604ml @20:51:12.07 Vacuum too high -- waiting up to 40s @20:51:13.66 delay 3 seconds @20:51:13.97 Intake @23.1psia, vacuum from 16.6 to 20.6psi @20:51:14.00 SS.reconfigure SSinhale.with maxSpeed: 32 @20:51:14.08 SS.seek 25ml @20:51:16.68 ATV.dial :bleach @20:51:17.35 AS.seek 0.589ml @20:51:20.68 delay 3 seconds @20:51:21.33 Stroke completed -- waiting for pressure to equalize ... @20:51:23.69 ATV.dial :ASV @20:51:24.77 ASV.dial :wasteR @20:51:25.40 AS.seek 0ml @20:51:25.84 Intake.select :close @20:51:26.02 SSV.dial :exhaust @20:51:27.48 Exhaust.select :open @20:51:27.62 SS.reconfigure SSexhale @20:51:27.70 SS.seek 0ml @20:51:37.27 Exhaust.select :close @20:51:37.41 SSV.dial CBV @20:51:37.44 CBV.dial SSV @20:51:38.32 Allowing 130s for 25.0ml of remaining 1000.0ml @20:51:38.34 Intake.select :open @20:51:40.55 Intake @25.1psia, vacuum from 18.6 to 22.6psi @20:51:40.57 SS.reconfigure SSinhale.with maxSpeed: 32 @20:51:40.67 SS.seek 25ml @20:52:35.78 Stroke completed -- waiting for pressure to equalize ... @20:52:38.67 -> exit @20:52:38.69 Session Ended @20:52:42.17 Intake.select :close @20:52:42.33 SSV.dial :exhaust @20:52:43.20 Exhaust.select :open @20:52:43.32 SS.reconfigure SSexhale @20:52:43.39 SS.seek 0ml @20:52:53.05 Exhaust.select :close @20:52:53.17 SSV.dial CBV @20:52:53.20 CBV.dial SSV @20:52:54.07 Allowing 130s for 25.0ml of remaining 975.0ml @20:52:54.08 Intake.select :open @20:52:56.27 Intake @25.1psia, vacuum from 18.6 to 22.6psi @20:52:56.29 SS.reconfigure SSinhale.with maxSpeed: 32 @20:52:56.34 SS.seek 25ml @20:53:20.85 Vacuum too high -- waiting up to 40s @20:53:21.70 Intake @23.2psia, vacuum from 16.7 to 20.7psi @20:53:21.72 SS.reconfigure SSinhale.with maxSpeed: 29 @20:53:21.77 SS.seek 25ml @20:53:24.20 delay 1:00 @20:53:55.51 Stroke completed -- waiting for pressure to equalize ... @20:54:00.08 Intake.select :close @20:54:00.24 SSV.dial :exhaust @20:54:01.11 Exhaust.select :open @20:54:01.22 SS.reconfigure SSexhale @20:54:01.28 SS.seek 0ml @20:54:10.94 Exhaust.select :close @20:54:11.06 SSV.dial CBV @20:54:11.09 CBV.dial SSV @20:54:11.96 Allowing 130s for 25.0ml of remaining 950.0ml @20:54:11.97 Intake.select :open @20:54:14.15 Intake @25.0psia, vacuum from 18.5 to 22.5psi @20:54:14.17 SS.reconfigure SSinhale.with maxSpeed: 29 @20:54:14.22 SS.seek 25ml @20:54:24.22 AFV.select :carrierSolution @20:54:24.33 delay 2 seconds @20:54:26.36 AS.reconfigure ASconfig @20:54:26.40 AS.seek 1ml @20:55:14.87 Stroke completed -- waiting for pressure to equalize ... @20:55:21.17 Intake.select :close @20:55:21.34 SSV.dial :exhaust @20:55:22.19 Exhaust.select :open @20:55:22.31 SS.reconfigure SSexhale @20:55:22.37 SS.seek 0ml @20:55:24.18 delay 1 second @20:55:25.19 AFV.select :holdingCoil @20:55:25.30 delay 1 second @20:55:26.32 ATV.dial ASV @20:55:26.34 ASV.dial :wasteR @20:55:26.36 AS.reconfigure ASslow1 @20:55:26.40 AS.seek 0ml @20:55:32.10 Exhaust.select :close @20:55:32.22 SSV.dial CBV @20:55:32.25 CBV.dial SSV @20:55:33.12 Allowing 130s for 25.0ml of remaining 925.0ml @20:55:33.13 Intake.select :open @20:55:35.31 Intake @25.1psia, vacuum from 18.6 to 22.6psi @20:55:35.33 SS.reconfigure SSinhale.with maxSpeed: 29 @20:55:35.38 SS.seek 25ml @20:56:35.98 Stroke completed -- waiting for pressure to equalize ... @20:56:42.39 Intake.select :close @20:56:42.55 SSV.dial :exhaust @20:56:43.41 Exhaust.select :open @20:56:43.52 SS.reconfigure SSexhale @20:56:43.57 SS.seek 0ml @20:56:53.29 Exhaust.select :close @20:56:53.41 SSV.dial CBV @20:56:53.44 CBV.dial SSV @20:56:54.29 Allowing 130s for 25.0ml of remaining 900.0ml @20:56:54.31 Intake.select :open @20:56:56.49 Intake @25.0psia, vacuum from 18.5 to 22.5psi @20:56:56.51 SS.reconfigure SSinhale.with maxSpeed: 29 @20:56:56.56 SS.seek 25ml @20:57:49.85 Vacuum too high -- waiting up to 40s @20:57:51.46 Intake @23.2psia, vacuum from 16.7 to 20.7psi @20:57:51.48 SS.reconfigure SSinhale.with maxSpeed: 26 @20:57:51.53 SS.seek 25ml @20:58:00.23 Stroke completed -- waiting for pressure to equalize ... @20:58:05.36 Intake.select :close @20:58:05.53 SSV.dial :exhaust @20:58:06.38 Exhaust.select :open @20:58:06.49 SS.reconfigure SSexhale @20:58:06.54 SS.seek 0ml @20:58:15.98 Exhaust.select :close @20:58:16.10 SSV.dial CBV @20:58:16.13 CBV.dial SSV @20:58:16.99 Allowing 130s for 25.0ml of remaining 875.0ml @20:58:17.00 Intake.select :open @20:58:19.72 Intake @24.9psia, vacuum from 18.4 to 22.4psi @20:58:19.74 SS.reconfigure SSinhale.with maxSpeed: 26 @20:58:19.79 SS.seek 25ml @20:58:47.77 delay 20 seconds @20:59:07.79 ATV.dial :air3 @20:59:09.34 AS.seek 0.59ml @20:59:27.67 Stroke completed -- waiting for pressure to equalize ... @20:59:34.09 Intake.select :close @20:59:34.25 SSV.dial :exhaust @20:59:35.10 Exhaust.select :open @20:59:35.21 SS.reconfigure SSexhale @20:59:35.27 SS.seek 0ml @20:59:44.72 Exhaust.select :close @20:59:44.84 SSV.dial CBV @20:59:44.87 CBV.dial SSV @20:59:45.74 Allowing 130s for 25.0ml of remaining 850.0ml @20:59:45.75 Intake.select :open @20:59:47.94 Intake @25.0psia, vacuum from 18.5 to 22.5psi @20:59:47.96 SS.reconfigure SSinhale.with maxSpeed: 26 @20:59:48.01 SS.seek 25ml @21:00:56.23 Stroke completed -- waiting for pressure to equalize ... @21:01:03.88 Intake.select :close @21:01:04.04 SSV.dial :exhaust @21:01:04.90 Exhaust.select :open @21:01:05.01 SS.reconfigure SSexhale @21:01:05.06 SS.seek 0ml @21:01:08.31 delay 3 seconds @21:01:11.33 ATV.dial :ASV @21:01:12.77 ASV.dial :wasteR @21:01:12.86 AS.seek 0.09ml @21:01:14.62 Exhaust.select :close @21:01:14.74 SSV.dial CBV @21:01:14.77 CBV.dial SSV @21:01:15.65 Allowing 130s for 25.0ml of remaining 825.0ml @21:01:15.66 Intake.select :open @21:01:17.84 Intake @25.0psia, vacuum from 18.5 to 22.5psi @21:01:17.86 SS.reconfigure SSinhale.with maxSpeed: 26 @21:01:17.91 SS.seek 25ml @21:02:26.03 Stroke completed -- waiting for pressure to equalize ... @21:02:34.22 Intake.select :close @21:02:34.38 SSV.dial :exhaust @21:02:35.24 Exhaust.select :open @21:02:35.36 SS.reconfigure SSexhale @21:02:35.42 SS.seek 0ml @21:02:44.95 Exhaust.select :close @21:02:45.07 SSV.dial CBV @21:02:45.10 CBV.dial SSV @21:02:45.97 Allowing 130s for 25.0ml of remaining 800.0ml @21:02:45.98 Intake.select :open @21:02:48.17 Intake @24.9psia, vacuum from 18.4 to 22.4psi @21:02:48.18 SS.reconfigure SSinhale.with maxSpeed: 26 @21:02:48.24 SS.seek 25ml @21:02:53.71 delay 30 seconds @21:03:10.44 Vacuum too high -- waiting up to 40s @21:03:11.54 Intake @23.2psia, vacuum from 16.7 to 20.7psi @21:03:11.57 SS.reconfigure SSinhale.with maxSpeed: 23 @21:03:11.62 SS.seek 25ml @21:03:23.73 ATV.dial :extSrc @21:03:24.52 delay 3 seconds @21:03:27.54 ATV.dial :ElutionWater @21:03:28.70 AS.seek 0.12ml @21:03:35.01 ATV.dial :air3 @21:03:35.64 AS.seek 0.13ml @21:03:37.96 delay 2 seconds @21:03:39.98 ATV.dial :bleach @21:03:40.47 AS.seek 0.23ml @21:04:00.84 delay 3 seconds @21:04:03.60 Stroke completed -- waiting for pressure to equalize ... @21:04:03.85 ATV.dial :air3 @21:04:04.41 delay 3 seconds @21:04:07.49 AS.seek 0.255ml @21:04:10.63 Intake.select :close @21:04:10.80 SSV.dial :exhaust @21:04:11.66 Exhaust.select :open @21:04:11.82 SS.reconfigure SSexhale @21:04:11.88 SS.seek 0ml @21:04:12.74 delay 3 seconds @21:04:15.75 ATV.dial :bleach @21:04:16.38 AS.seek 0.24ml @21:04:19.66 delay 3 seconds @21:04:21.54 Exhaust.select :close @21:04:21.66 SSV.dial CBV @21:04:21.69 CBV.dial SSV @21:04:22.57 Allowing 130s for 25.0ml of remaining 775.0ml @21:04:22.58 Intake.select :open @21:04:22.67 ATV.dial :ASV @21:04:23.73 ASV.dial :SPEdelivery @21:04:24.11 AFV.select :carrierSolution @21:04:24.23 delay 2 seconds @21:04:24.81 Intake @24.8psia, vacuum from 18.3 to 22.3psi @21:04:24.83 SS.reconfigure SSinhale.with maxSpeed: 23 @21:04:24.88 SS.seek 25ml @21:04:26.26 AS.reconfigure ASconfig @21:04:26.31 AS.seek 0.5ml @21:04:41.57 delay 1 second @21:04:42.58 AFV.select :holdingCoil @21:04:42.74 AS.reconfigure ASslow1 @21:04:42.79 AS.seek 0.17ml @21:05:41.57 Stroke completed -- waiting for pressure to equalize ... @21:05:49.15 Intake.select :close @21:05:49.32 SSV.dial :exhaust @21:05:49.46 SPE.hold 2:46:40,95C @21:05:49.50 delay 3:00 @21:05:50.21 Exhaust.select :open @21:05:50.32 SS.reconfigure SSexhale @21:05:50.38 SS.seek 0ml @21:06:00.07 Exhaust.select :close @21:06:00.19 SSV.dial CBV @21:06:00.22 CBV.dial SSV @21:06:01.13 Allowing 130s for 25.0ml of remaining 750.0ml @21:06:01.14 Intake.select :open @21:06:03.32 Intake @25.0psia, vacuum from 18.5 to 22.5psi @21:06:03.34 SS.reconfigure SSinhale.with maxSpeed: 23 @21:06:03.39 SS.seek 25ml @21:07:19.99 Stroke completed -- waiting for pressure to equalize ... @21:07:28.28 Intake.select :close @21:07:28.44 SSV.dial :exhaust @21:07:29.35 Exhaust.select :open @21:07:29.46 SS.reconfigure SSexhale @21:07:29.51 SS.seek 0ml @21:07:39.17 Exhaust.select :close @21:07:39.29 SSV.dial CBV @21:07:39.32 CBV.dial SSV @21:07:40.24 Allowing 130s for 25.0ml of remaining 725.0ml @21:07:40.25 Intake.select :open @21:07:42.43 Intake @24.7psia, vacuum from 18.2 to 22.2psi @21:07:42.45 SS.reconfigure SSinhale.with maxSpeed: 23 @21:07:42.50 SS.seek 25ml @21:08:23.44 Vacuum too high -- waiting up to 40s @21:08:25.04 Intake @23.4psia, vacuum from 16.9 to 20.9psi @21:08:25.06 SS.reconfigure SSinhale.with maxSpeed: 21 @21:08:25.11 SS.seek 25ml @21:08:49.52 AFV.select :carrierSolution @21:08:49.63 delay 2 seconds @21:08:51.66 AS.reconfigure ASconfig @21:08:51.71 AS.seek 1ml @21:09:04.67 Stroke completed -- waiting for pressure to equalize ... @21:09:11.63 Intake.select :close @21:09:11.79 SSV.dial :exhaust @21:09:12.68 Exhaust.select :open @21:09:12.80 SS.reconfigure SSexhale @21:09:12.86 SS.seek 0ml @21:09:22.42 Exhaust.select :close @21:09:22.54 SSV.dial CBV @21:09:22.57 CBV.dial SSV @21:09:23.47 Allowing 130s for 25.0ml of remaining 700.0ml @21:09:23.48 Intake.select :open @21:09:25.67 Intake @24.7psia, vacuum from 18.2 to 22.2psi @21:09:25.69 SS.reconfigure SSinhale.with maxSpeed: 21 @21:09:25.74 SS.seek 25ml @21:09:39.71 delay 1 second @21:09:40.72 AFV.select :holdingCoil @21:09:40.83 delay 1 second @21:09:41.85 ASV.dial :wasteD @21:09:42.98 AS.seek 0ml @21:10:40.76 Priming Eluate @21:10:40.77 AFV.select :carrierSolution @21:10:40.88 delay 2 seconds @21:10:42.91 AS.seek 0.5ml @21:10:50.02 Stroke completed -- waiting for pressure to equalize ... @21:10:58.98 Intake.select :close @21:10:59.14 SSV.dial :exhaust @21:11:00.02 Exhaust.select :open @21:11:00.15 SS.reconfigure SSexhale @21:11:00.21 SS.seek 0ml @21:11:09.65 Exhaust.select :close @21:11:09.77 SSV.dial CBV @21:11:09.80 CBV.dial SSV @21:11:10.67 Allowing 130s for 25.0ml of remaining 675.0ml @21:11:10.68 Intake.select :open @21:11:11.94 delay 1 second @21:11:12.86 Intake @24.5psia, vacuum from 18.0 to 22.0psi @21:11:12.89 SS.reconfigure SSinhale.with maxSpeed: 21 @21:11:12.94 SS.seek 25ml @21:11:12.99 AFV.select :holdingCoil @21:11:13.10 ATV.dial ASV @21:11:13.11 ASV.dial :SPErecovery @21:11:13.74 Swish #1 of 2 @21:11:13.82 AS.reconfigure ASslow0 @21:11:13.87 AS.seek 0.6ml @21:11:24.27 AS.seek 0.5ml @21:11:34.65 Swish #2 of 2 @21:11:34.74 AS.seek 0.6ml @21:11:45.22 AS.seek 0.5ml @21:11:55.57 delay 3 seconds @21:11:58.65 AS.reconfigure ASslow1 @21:11:58.70 AS.seek 0.684ml @21:12:20.68 Session Begun @21:12:25.74 -> showlog 100 @21:12:36.05 delay 3 seconds @21:12:37.34 Stroke completed -- waiting for pressure to equalize ... @21:12:39.08 Eluate primed @21:12:39.09 ATV.dial :waste2 @21:12:39.87 AS.reconfigure ASnorm @21:12:39.95 AS.seek 0ml @21:12:48.17 Intake.select :close @21:12:48.36 SSV.dial :exhaust @21:12:49.25 Exhaust.select :open @21:12:49.39 SS.reconfigure SSexhale @21:12:49.47 SS.seek 0ml @21:12:59.09 Exhaust.select :close @21:12:59.24 SSV.dial CBV @21:12:59.27 CBV.dial SSV @21:13:00.17 Allowing 130s for 25.0ml of remaining 650.0ml @21:13:00.20 Intake.select :open @21:13:02.40 Intake @24.9psia, vacuum from 18.4 to 22.4psi @21:13:02.43 SS.reconfigure SSinhale.with maxSpeed: 21 @21:13:02.52 SS.seek 25ml @21:13:14.76 SPE.coast @21:13:14.87 ASV.dial :SPErecovery @21:13:14.89 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep0821h1400ml.pcr @21:13:14.93 Building PCR Slug from ASV with MM2 mix, PP4 primer @21:13:14.96 Priming with carrier @21:13:14.98 ATV.dial ARV @21:13:16.00 ARV.dial :carrier @21:13:16.39 AS.seek 0.5ml @21:13:35.22 Vacuum too high -- waiting up to 40s @21:13:36.57 Intake @23.5psia, vacuum from 17.0 to 21.0psi @21:13:36.60 SS.reconfigure SSinhale.with maxSpeed: 19 @21:13:36.69 SS.seek 25ml @21:13:41.98 AFV.select :carrierSolution @21:13:42.12 delay 2 seconds @21:13:44.15 AS.reconfigure ASconfig @21:13:44.22 AS.seek 1ml @21:14:13.27 delay 1 second @21:14:14.29 AFV.select :holdingCoil @21:14:14.43 delay 1 second @21:14:15.45 ATV.dial :waste @21:14:16.99 AS.reconfigure ASnorm @21:14:17.07 AS.seek :empty @21:14:33.84 Stroke completed -- waiting for pressure to equalize ... @21:14:54.75 Intake.select :close @21:14:54.94 SSV.dial :exhaust @21:14:55.81 Exhaust.select :open @21:14:55.94 SS.reconfigure SSexhale @21:14:56.02 SS.seek 0ml @21:15:05.62 Exhaust.select :close @21:15:05.77 SSV.dial CBV @21:15:05.80 CBV.dial SSV @21:15:06.72 Allowing 130s for 25.0ml of remaining 625.0ml @21:15:06.74 Intake.select :open @21:15:07.83 AFV.select :carrierSolution @21:15:07.97 delay 2 seconds @21:15:08.95 Intake @24.9psia, vacuum from 18.4 to 22.4psi @21:15:08.97 SS.reconfigure SSinhale.with maxSpeed: 19 @21:15:09.06 SS.seek 25ml @21:15:10.00 AS.reconfigure ASconfig @21:15:10.07 AS.seek 0.5ml @21:15:39.21 delay 1 second @21:15:40.22 AFV.select :holdingCoil @21:15:40.35 Building Slug @21:15:40.36 ATV.dial :ARV @21:15:42.15 delay 2 seconds @21:15:44.17 ARV.dial :air1 @21:15:44.60 delay 2 seconds @21:15:46.69 AS.reconfigure ASslow2 @21:15:46.77 AS.seek 0.506ml @21:15:53.10 delay 2 seconds @21:15:55.13 ARV.dial :MM2 @21:15:56.71 delay 2 seconds @21:15:58.74 Adding MM2 @21:15:58.84 AS.seek 0.524ml @21:16:17.09 delay 25 seconds @21:16:42.09 Stroke completed -- waiting for pressure to equalize ... @21:16:42.13 ARV.dial :PP4 @21:16:42.57 Adding PP4 @21:16:42.58 delay 2 seconds @21:16:44.73 AS.seek 0.53ml @21:16:51.05 delay 10 seconds @21:17:01.14 ARV.dial :air3 @21:17:01.57 adding ARV air @21:17:01.59 delay 2 seconds @21:17:03.76 AS.seek 0.536ml @21:17:08.22 Intake.select :close @21:17:08.40 SSV.dial :exhaust @21:17:09.28 Exhaust.select :open @21:17:09.42 SS.reconfigure SSexhale @21:17:09.50 SS.seek 0ml @21:17:10.19 delay 5 seconds @21:17:15.21 ARV.dial :carrier @21:17:16.52 delay 7 seconds @21:17:19.29 Exhaust.select :close @21:17:19.44 SSV.dial CBV @21:17:19.47 CBV.dial SSV @21:17:20.37 Allowing 130s for 25.0ml of remaining 600.0ml @21:17:20.39 Intake.select :open @21:17:22.59 Intake @24.7psia, vacuum from 18.2 to 22.2psi @21:17:22.61 SS.reconfigure SSinhale.with maxSpeed: 19 @21:17:22.71 SS.seek 25ml @21:17:23.60 AS.seek 0.594ml @21:18:22.32 delay 5 seconds @21:18:27.34 ATV.dial :ASV @21:18:27.77 delay 2 seconds @21:18:29.86 AS.seek 0.6ml @21:18:36.38 delay 4 seconds @21:18:40.40 ATV.dial ARV @21:18:40.98 delay 2 seconds @21:18:41.06 Vacuum too high -- waiting up to 40s @21:18:42.40 Intake @23.0psia, vacuum from 16.5 to 20.5psi @21:18:42.43 SS.reconfigure SSinhale.with maxSpeed: 17 @21:18:42.52 SS.seek 25ml @21:18:43.01 ARV.dial :carrier @21:18:43.03 delay 2 seconds @21:18:45.14 AS.seek 0.65ml @21:18:59.41 Stroke completed -- waiting for pressure to equalize ... @21:19:30.43 SS.reconfigure SSconfig @21:19:30.51 SS.hold @21:19:30.67 Intake.select :close @21:19:30.86 ShallowSampler::Clogged in WCR -- progress too slow after 925.0ml @21:19:30.88 SSV.dial :exhaust @21:19:31.77 Exhaust.select :open @21:19:31.91 SS.reconfigure SSexhale @21:19:31.99 SS.seek 0ml @21:19:35.78 delay 4 seconds @21:19:39.81 ATV.dial PCR @21:19:40.45 ASV.dial :waste1 @21:19:41.25 ARV.dial :air1 @21:19:41.70 Positioning Slug by 0.324ml @21:19:41.81 Exhaust.select :close @21:19:41.86 AS.seek 0.326ml @21:19:42.04 Sampled 925.0ml @21:19:42.07 Drying puck before fixing for 4:00 @21:19:42.09 CBV.dial :waste2b @21:19:42.98 delay 1:00 @21:19:45.04 Gate.power :sampler,:OFF @21:20:43.01 CSV.dial :air @21:20:44.13 CS.reconfigure CSfast @21:20:44.21 CS.seek 10ml @21:20:49.16 -> exit @21:20:49.18 Session Ended @21:21:10.72 Pumping wcr puck from tube 2 up to 55psi... @21:21:10.74 CBV.dial SSV @21:21:12.17 CBV.dial between CSV and waste2b @21:21:13.26 CSV.dial between air and CTV @21:21:13.64 CS.seek 7.49ml @21:21:21.19 CTV.dial CSV,avoiding: :air @21:21:21.22 CSV.dial CTV @21:21:22.12 CS.reconfigure CSpressurize @21:21:22.17 CS.seek 0ml @21:21:42.03 CSV.dial :air @21:21:42.55 CS.reconfigure CSfast @21:21:42.61 CS.seek 10ml @21:22:08.91 CSV.dial between air and CTV @21:22:09.28 CS.seek 2.16ml @21:22:30.22 CTV.dial CSV,avoiding: :air @21:22:30.25 CSV.dial CTV @21:22:30.70 CS.reconfigure CSpressurize @21:22:30.76 CS.seek 0ml @21:22:32.21 CBV.dial :waste2b @21:22:32.58 CS.reconfigure CSconfig @21:22:32.63 CS.seek :empty @21:22:38.47 delay 1:00 @21:23:38.50 CTV.dial :air,avoiding: :intake @21:23:40.00 Fix #1 of 2 (with rnal) for 2:00 @21:23:40.02 CSV.dial CSR @21:23:40.59 Pulling 2ml of CSR.rnal @21:23:40.61 CSR.select :rnal @21:23:40.73 CS.seek 2ml @21:23:46.82 delay 5 seconds @21:23:51.84 CSR.select :air @21:23:51.97 CS.reconfigure CSfast @21:23:52.02 CS.seek 10ml @21:24:13.28 Pumping wcr puck from tube 2 up to 55psi... @21:24:13.29 CBV.dial SSV @21:24:14.70 CBV.dial between CSV and waste2b @21:24:15.79 CSV.dial between air and CTV @21:24:16.48 CS.seek 7.431ml @21:24:24.07 CTV.dial CSV,avoiding: :air @21:24:24.10 CSV.dial CTV @21:24:25.64 CS.reconfigure CSpressurize @21:24:25.70 CS.seek 0ml @21:24:44.90 delay 2:00 @21:25:08.04 delay 5 seconds @21:25:13.06 ATV.dial ARV @21:25:13.83 ASV.dial :PCRlock @21:25:14.21 delay 5 seconds @21:25:19.24 Running Tt1 Profile60C300 thermocycling @21:25:19.25 PCR.program Profile60C300 @21:25:19.39 Profile60C300 should complete in < 1:45:21 @21:25:19.40 PCR.run 2:16:41.25 @21:26:44.93 CBV.dial :waste2b @21:26:45.29 CS.reconfigure CSconfig @21:26:45.34 CS.seek :empty @21:26:46.79 delay 1:00 @21:27:46.82 CTV.dial :air,avoiding: :intake @21:27:48.32 Fix #2 of 2 (with rnal) for 2:00 @21:27:48.34 CSV.dial CSR @21:27:48.88 Pulling 2ml of CSR.rnal @21:27:48.90 CSR.select :rnal @21:27:49.02 CS.seek 2ml @21:27:55.13 delay 5 seconds @21:28:00.15 CSR.select :air @21:28:00.28 CS.reconfigure CSfast @21:28:00.34 CS.seek 10ml @21:28:21.82 Pumping wcr puck from tube 2 up to 55psi... @21:28:21.83 CBV.dial SSV @21:28:23.24 CBV.dial between CSV and waste2b @21:28:24.34 CSV.dial between air and CTV @21:28:25.03 CS.seek 7.376ml @21:28:32.63 CTV.dial CSV,avoiding: :air @21:28:32.66 CSV.dial CTV @21:28:34.21 CS.reconfigure CSpressurize @21:28:34.27 CS.seek 0ml @21:28:52.37 delay 2:00 @21:30:52.39 CBV.dial :waste2b @21:30:52.77 CS.reconfigure CSconfig @21:30:52.83 CS.seek :empty @21:30:55.19 delay 1:00 @21:31:55.21 CTV.dial :air,avoiding: :intake @21:31:56.78 Storing wcr puck in Collection Clamp to tube 1 @21:31:56.98 SC.reconfigure SCtight @21:31:57.03 SC.seek 1 @21:31:59.20 SC.reconfigure SCloose @21:31:59.44 Elbow.reconfigure TightElbow @21:31:59.49 Elbow.seek 1 @21:32:01.43 Forearm.reconfigure TightForearm @21:32:01.49 Forearm.seek 1 + 700 counts @21:32:02.55 SE.reconfigure SEpick @21:32:02.62 SE.seek beamBreak @21:32:06.24 SE.configure SEconfig @21:32:06.45 Elbow.reconfigure LimpElbow @21:32:06.50 Forearm.reconfigure LooseForearm @21:32:06.55 Forearm.seek 1 @21:32:06.89 Elbow.hold @21:32:06.97 Elbow.reconfigure TightElbow @21:32:07.08 SE.seek 1.08 Pucks below Top Plate @21:32:07.61 Forearm.force -20 @21:32:07.66 Forearm.seek 1 + 326 counts @21:32:08.00 Forearm.reconfigure TightForearm @21:32:08.06 Forearm.seek 1 + 700 counts @21:32:08.37 SE.seek 2.08 Pucks below Top Plate @21:32:09.19 Forearm.reconfigure LooseForearm @21:32:09.25 Forearm.seek :retract @21:32:10.20 Elbow.seek CC @21:32:11.90 Elbow.reconfigure LooseElbow @21:32:12.02 Forearm.seek CC @21:32:12.99 Hand.close @21:32:13.79 CC.seek :open @21:32:18.34 Forearm.seek :retract @21:32:19.36 Elbow.reconfigure TightElbow @21:32:19.41 Elbow.seek 1 @21:32:21.40 Elbow.reconfigure LimpElbow @21:32:21.47 Forearm.seek 1 @21:32:22.48 Elbow.hold @21:32:22.56 Elbow.reconfigure TightElbow @21:32:22.61 Hand.open @21:32:23.56 Forearm.force -20 @21:32:23.61 Forearm.seek 1 + 348 counts @21:32:23.95 Forearm.reconfigure TightForearm @21:32:24.01 Forearm.seek 1 + 700 counts @21:32:24.39 SE.seek :down @21:32:25.54 Forearm.reconfigure LooseForearm @21:32:25.59 Forearm.seek :retract @21:32:27.17 SE.coast @21:32:27.21 SC.coast @21:32:27.26 Starting finalPurge @21:32:27.27 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:32:27.35 Hand.open @21:32:27.68 Elbow.seek FlushPuck::Garage @21:32:29.63 Elbow.reconfigure LooseElbow @21:32:29.74 Forearm.seek :garage @21:32:31.25 Hand.close @21:32:32.18 Forearm.seek :retract @21:32:33.20 Elbow.reconfigure TightElbow @21:32:33.25 Elbow.seek CC @21:32:36.10 Elbow.reconfigure LooseElbow @21:32:36.21 Forearm.reconfigure TightForearm @21:32:36.26 Forearm.seek CC @21:32:37.61 CC.seek :closed @21:32:41.88 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.29 @21:32:41.96 Hand.open @21:32:42.74 Forearm.reconfigure LooseForearm @21:32:42.79 Forearm.seek :retract @21:32:43.81 Flushing the Collection Valve::Manifold @21:32:43.98 CSV.dial CSR @21:32:44.52 Pulling 5ml of CSR.flush @21:32:44.53 CSR.select :flush @21:32:44.66 CS.seek 5ml @21:32:58.85 delay 5 seconds @21:33:03.87 CSR.select :air @21:33:04.04 CS.reconfigure CSfast @21:33:04.09 CS.seek 7.5ml @21:33:11.40 CSV.dial :waste @21:33:12.88 CS.reconfigure CSconfig @21:33:12.93 CS.seek 0ml @21:33:33.10 Flushing lower manifold's waste1 port @21:33:33.24 CSV.dial :flush @21:33:33.87 CS.reconfigure CSfast @21:33:33.93 CS.seek 5ml @21:33:47.79 delay 5 seconds @21:33:52.81 CSV.dial :air @21:33:53.39 CS.seek 7ml @21:33:59.50 CSV.dial CTV @21:33:59.53
CTV.dial CSV @21:34:01.10 CBV.dial :waste1 @21:34:01.96 CS.seek 0ml @21:34:20.85 Flushing lower manifold's waste2b port @21:34:20.99 CSV.dial :flush @21:34:21.83 CS.seek 5ml @21:34:35.63 delay 5 seconds @21:34:40.65 CSV.dial :air @21:34:41.25 CS.seek 7ml @21:34:47.29 CSV.dial CTV @21:34:47.32
CTV.dial CSV @21:34:47.82 CBV.dial :waste2b @21:34:48.69 CS.seek 0ml @21:35:07.51 Flushing lower manifold's waste1 port @21:35:07.52 CSV.dial CSR @21:35:08.69 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @21:35:08.82 CS.reconfigure CSconfig @21:35:08.87 CS.seek 4ml @21:35:20.10 delay 5 seconds @21:35:25.11 CSR.select :air @21:35:25.24 CS.reconfigure CSfast @21:35:25.29 CS.seek 10ml @21:35:41.60 CSV.dial CTV @21:35:41.63
CTV.dial CSV @21:35:42.17 CBV.dial :waste1 @21:35:43.03 CS.seek 0ml @21:36:09.67 Flushing lower manifold's waste2b port @21:36:09.68 CSV.dial CSR @21:36:10.37 Pulling 4ml of CSR.mfbkill @21:36:10.38 CSR.select :mfbkill @21:36:10.51 CS.reconfigure CSconfig @21:36:10.56 CS.seek 4ml @21:36:21.85 delay 5 seconds @21:36:26.87 CSR.select :air @21:36:27.00 CS.reconfigure CSfast @21:36:27.05 CS.seek 10ml @21:36:43.32 CSV.dial CTV @21:36:43.35
CTV.dial CSV @21:36:43.85 CBV.dial :waste2b @21:36:44.72 CS.seek 0ml @21:37:11.39 Killing upper manifold @21:37:11.40 CSV.dial CSR @21:37:11.98 Pulling 4ml of CSR.mfbkill @21:37:11.99 CSR.select :mfbkill @21:37:12.12 CS.reconfigure CSconfig @21:37:12.16 CS.seek 4ml @21:37:23.35 delay 5 seconds @21:37:28.36 CSR.select :air @21:37:28.49 CS.reconfigure CSfast @21:37:28.54 CS.seek 10ml @21:37:44.67 CSV.dial CBV @21:37:44.70
CBV.dial CSV @21:37:45.89 CTV.dial :air @21:37:47.40 CS.seek 0ml @21:38:13.70 delay 7 seconds @21:38:20.73 CS.seek 3ml @21:38:29.31 CSV.dial :waste @21:38:29.81 CS.seek 0ml @21:38:38.39 Flushing upper manifold @21:38:38.53 CSV.dial :flush @21:38:39.04 CS.seek 5ml @21:38:52.76 delay 5 seconds @21:38:57.77 CSV.dial :air @21:38:58.47 CS.seek 7ml @21:39:04.60 CSV.dial CBV @21:39:04.62
CBV.dial CSV @21:39:05.79 CTV.dial :air @21:39:05.81 CS.seek 0ml @21:39:24.45 delay 7 seconds @21:39:31.48 CS.seek 3ml @21:39:40.03 CSV.dial :waste @21:39:40.54 CS.seek 0ml @21:39:49.33 CSV.dial :flush @21:39:50.41 CS.seek 3ml @21:39:58.95 delay 5 seconds @21:40:03.96 CSV.dial :air @21:40:04.53 CS.seek 5ml @21:40:10.51 CSV.dial :air @21:40:10.53 CS.seek 0ml @21:40:24.07 delay 7 seconds @21:40:31.10 CS.seek 3ml @21:40:39.72 CSV.dial :waste @21:40:40.57 CS.seek 0ml @21:40:49.36 Flushing lower manifold's waste1 port @21:40:49.50 CSV.dial :flush @21:40:50.02 CS.seek 5ml @21:41:03.73 delay 5 seconds @21:41:08.74 CSV.dial :air @21:41:09.32 CS.seek 7ml @21:41:15.39 CSV.dial CTV @21:41:15.42
CTV.dial CSV @21:41:16.98 CBV.dial :waste1 @21:41:17.51 CS.seek 0ml @21:41:36.14 Flushing lower manifold's waste2b port @21:41:36.28 CSV.dial :flush @21:41:37.13 CS.seek 5ml @21:41:50.83 delay 5 seconds @21:41:55.84 CSV.dial :air @21:41:56.44 CS.seek 7ml @21:42:02.45 CSV.dial CTV @21:42:02.47
CTV.dial CSV @21:42:02.98 CBV.dial :waste2b @21:42:03.85 CS.seek 0ml @21:42:22.45 Garaging FlushPuck in Collection Clamp @21:42:22.53 Elbow.reconfigure TightElbow @21:42:22.58 Elbow.seek CC @21:42:22.81 Elbow.reconfigure LooseElbow @21:42:22.92 Forearm.seek CC @21:42:23.87 Hand.close @21:42:24.70 CC.seek :open @21:42:29.11 Forearm.seek :retract @21:42:30.12 Elbow.reconfigure TightElbow @21:42:30.17 Elbow.seek :garage @21:42:33.35 Elbow.reconfigure LooseElbow @21:42:33.46 Forearm.reconfigure TightForearm @21:42:33.51 Forearm.seek :garage @21:42:34.67 Hand.open @21:42:35.50 Forearm.reconfigure LooseForearm @21:42:35.55 Forearm.seek :retract @21:42:36.62 Starting initialPurge @21:42:36.63 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:42:36.71 Hand.open @21:42:37.00 Elbow.reconfigure TightElbow @21:42:37.05 Elbow.seek FlushPuck::Garage @21:42:37.17 Elbow.reconfigure LooseElbow @21:42:37.28 Forearm.seek :garage @21:42:38.32 Hand.close @21:42:39.83 Forearm.seek :retract @21:42:40.91 Elbow.reconfigure TightElbow @21:42:40.96 Elbow.seek CC @21:42:43.87 Elbow.reconfigure LooseElbow @21:42:43.97 Forearm.reconfigure TightForearm @21:42:44.02 Forearm.seek CC @21:42:45.33 CC.seek :closed @21:42:49.61 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.46 @21:42:49.69 Hand.open @21:42:50.50 Forearm.reconfigure LooseForearm @21:42:50.55 Forearm.seek :retract @21:42:51.54 Flushing the Collection Valve::Manifold @21:42:51.69 CSV.dial CSR @21:42:52.24 Pulling 5ml of CSR.flush @21:42:52.25 CSR.select :flush @21:42:52.38 CS.reconfigure CSconfig @21:42:52.43 CS.seek 5ml @21:43:06.23 delay 5 seconds @21:43:11.25 CSR.select :air @21:43:11.43 CS.reconfigure CSfast @21:43:11.49 CS.seek 7.5ml @21:43:18.75 CSV.dial :waste @21:43:20.33 CS.reconfigure CSconfig @21:43:20.38 CS.seek 0ml @21:43:40.51 Purging external Intake @21:43:40.66 CSV.dial :flush @21:43:41.18 CS.reconfigure CSfast @21:43:41.23 CS.seek 10ml @21:44:07.45 delay 5 seconds @21:44:12.47 CSV.dial CBV @21:44:12.51
CBV.dial CSV @21:44:13.53 CTV.dial :intake @21:44:14.37 Intake.select :open @21:44:14.51 CS.seek 0ml @21:44:41.47 Intake.select :close @21:44:41.59 CTV.dial :air @21:44:42.44 Flushing upper manifold @21:44:42.57 CSV.dial :flush @21:44:43.41 CS.seek 5ml @21:44:56.98 delay 5 seconds @21:45:01.99 CSV.dial :air @21:45:02.57 CS.seek 7ml @21:45:08.58 CSV.dial CBV @21:45:08.61
CBV.dial CSV @21:45:09.90 CTV.dial :air @21:45:09.92 CS.seek 0ml @21:45:28.70 delay 7 seconds @21:45:35.72 CS.seek 3ml @21:45:44.32 CSV.dial :waste @21:45:44.82 CS.seek 0ml @21:45:53.48 CSV.dial :flush @21:45:53.99 CS.seek 3ml @21:46:02.51 delay 5 seconds @21:46:08.12 CSV.dial :air @21:46:08.69 CS.seek 5ml @21:46:14.80 CSV.dial :air @21:46:14.82 CS.seek 0ml @21:46:28.67 delay 7 seconds @21:46:35.70 CS.seek 3ml @21:46:44.35 CSV.dial :waste @21:46:45.21 CS.seek 0ml @21:46:53.95 Garaging FlushPuck in Collection Clamp @21:46:54.03 Elbow.reconfigure TightElbow @21:46:54.08 Elbow.seek CC @21:46:54.32 Elbow.reconfigure LooseElbow @21:46:54.43 Forearm.seek CC @21:46:55.43 Hand.close @21:46:56.18 CC.seek :open @21:47:00.55 Forearm.seek :retract @21:47:01.53 Elbow.reconfigure TightElbow @21:47:01.58 Elbow.seek :garage @21:47:04.74 Elbow.reconfigure LooseElbow @21:47:04.85 Forearm.reconfigure TightForearm @21:47:04.90 Forearm.seek :garage @21:47:06.08 Hand.open @21:47:06.88 Forearm.reconfigure LooseForearm @21:47:06.93 Forearm.seek :retract @21:47:07.99 Completed [mbaPhase] begun 18:04:09 @21:47:08.01 Extending 18tun1 until completion of MFBPCR @22:20:29.81 Session Begun @22:20:55.75 -> showlog @22:21:12.33 -> exit @22:21:12.34 Session Ended @22:49:56.35 PCR.off @22:49:57.96 PCR for Tt1 completed -- closed /var/log/esp/Tt118sep0821h1400ml.pcr @22:49:58.03 Cleaning PCR @22:49:58.04 AFV.select :carrierSolution @22:49:58.16 delay 2 seconds @22:50:00.19 AS.reconfigure ASconfig @22:50:00.24 AS.seek 0.5ml @22:50:10.55 delay 1 second @22:50:11.56 AFV.select :holdingCoil @22:50:11.67 ASV.dial :waste1 @22:50:12.05 ATV.dial :air3 @22:50:13.34 AS.reconfigure ASslow1 @22:50:13.39 AS.seek 0.51ml @22:50:15.69 delay 2 seconds @22:50:17.71 ATV.dial :bleach @22:50:18.34 AS.seek 0.81ml @22:51:19.05 delay 3 seconds @22:51:22.07 ATV.dial :air3 @22:51:22.63 delay 3 seconds @22:51:25.72 AS.seek 0.835ml @22:51:31.07 delay 3 seconds @22:51:34.09 ATV.dial :bleach @22:51:34.73 AS.seek 0.82ml @22:51:38.06 delay 3 seconds @22:51:41.07 ARV.dial :waste @22:51:41.69 delay 3 seconds @22:51:44.71 ATV.dial :ARV @22:51:45.59 AS.reconfigure ASconfig @22:51:45.65 AS.seek 0.47ml @22:52:06.04 AFV.select :carrierSolution @22:52:06.16 delay 2 seconds @22:52:08.19 AS.seek 1ml @22:52:38.99 delay 1 second @22:52:40.00 AFV.select :holdingCoil @22:52:40.11 delay 1 second @22:52:41.14 AS.reconfigure ASfast @22:52:41.19 AS.seek 0ml @22:53:02.40 Slide::Error in MFBPCR -- Analytical Syringe speedErr at 0.25ml @22:53:02.42 Waiting to Resume from Checkpoint "Analytical Syringe speedErr at 0.25ml" @22:53:02.43 BadNews.email "Analytical Syringe speedErr at 0.25ml",Subject: "Slide::Error in MFBPCR" @10:18:21.03CEST09-Sep-18 Session Begun @10:18:44.20 -> showlog 100 @10:19:48.99 -> MFBPCRThread.resume @10:19:49.01 NameError in bkha -- uninitialized constant MFBPCRThread @10:20:15.07 -> MFBPCR.resume @10:20:15.10 NameError in bkha -- uninitialized constant MFBPCR @10:20:30.32 -> MFBPCR.Thread resume @10:20:30.34 NameError in bkha -- uninitialized constant MFBPCR @10:20:50.97 -> MFBPCRthread.resume @10:20:50.99 NameError in bkha -- uninitialized constant MFBPCRthread @10:21:22.95 -> MFBPCR.thread.resume @10:21:22.97 NameError in bkha -- uninitialized constant MFBPCR @10:21:34.84 -> MFBPCRThread.resume @10:21:34.86 NameError in bkha -- uninitialized constant MFBPCRThread @10:23:21.59 -> Thread[:MFBPCR].recover @10:23:21.63 Recovering Thread after Analytical Syringe speedErr at 0.25ml at 22:52:54 @10:23:21.70 AS.seek 0ml @10:23:22.12 Slide::Error in MFBPCR -- Analytical Syringe speedErr at 0.25ml @10:23:22.14 Waiting to Resume from Checkpoint "Analytical Syringe speedErr at 0.25ml" @10:23:22.17 BadNews.email "Analytical Syringe speedErr at 0.25ml",Subject: "Slide::Error in MFBPCR" @10:23:32.22 -> AFV @10:23:39.03 -> ATV @10:23:43.30 -> ARV @10:24:06.60 -> AFV.legnd @10:24:06.61 AFV.select :legnd @10:24:06.65 Solenoid::Error in bkha -- Invalid Analytical Fluidic Valve State: legnd @10:24:12.64 -> AFV.legend @10:24:30.36 -> AFV.carrier @10:24:30.38 AFV.select :carrier @10:24:37.94 -> AFV.coil @10:24:37.96 AFV.select :coil @10:24:58.23 -> Thread[:MFBPCR].recover @10:24:58.26 Recovering Thread after Analytical Syringe speedErr at 0.25ml at 10:23:21 @10:24:58.30 AS.seek 0ml @10:24:58.76 Slide::Error in MFBPCR -- Analytical Syringe speedErr at 0.25ml @10:24:58.77 Waiting to Resume from Checkpoint "Analytical Syringe speedErr at 0.25ml" @10:24:58.80 BadNews.email "Analytical Syringe speedErr at 0.25ml",Subject: "Slide::Error in MFBPCR" @10:25:16.33 -> ATV @10:25:27.17 -> ATV.waste @10:25:27.19 ATV.dial :waste @10:25:39.67 -> AS.empty @10:25:39.70 AS.reconfigure ASconfig @10:25:39.78 AS.seek :empty @10:25:42.61 AS.hold @10:25:42.92 UserAbort in bkha -- Analytical Syringe seek to empty Aborted! @10:25:57.84 -> ATV.ARV @10:25:57.85 ATV.dial ARV @10:26:07.80 -> ARV @10:26:22.12 -> ARV @10:26:26.27 -> ARV.lengend @10:26:26.29 NoMethodError in bkha -- undefined method `lengend' for ARV:Shaft @10:26:32.29 -> ARV.legend @10:27:04.63 -> ARV.carrier @10:27:04.65 ARV.dial :carrier @10:27:11.10 -> ARV.waste @10:27:11.13 ARV.dial :waste @10:27:34.02 -> Thread[:MFBPCR].recover @10:27:34.05 Recovering Thread after Analytical Syringe speedErr at 0.25ml at 10:24:58 @10:27:34.09 AS.reconfigure ASfast @10:27:34.20 AS.seek 0ml @10:27:40.13 AFV.select :carrierSolution @10:27:40.27 delay 2 seconds @10:27:42.30 AS.reconfigure ASconfig @10:27:42.38 AS.seek 1ml @10:28:40.18 delay 1 second @10:28:41.21 AFV.select :holdingCoil @10:28:41.34 delay 1 second @10:28:42.37 AS.reconfigure ASfast @10:28:42.44 AS.seek 0ml @10:29:09.82 AFV.select :carrierSolution @10:29:09.96 delay 2 seconds @10:29:11.99 AS.reconfigure ASconfig @10:29:12.06 AS.seek 1ml @10:30:09.92 delay 1 second @10:30:10.94 AFV.select :holdingCoil @10:30:11.06 delay 1 second @10:30:12.09 AS.reconfigure ASfast @10:30:12.16 AS.seek 0ml @10:30:39.65 ARV.dial between carrier and air1 @10:30:40.19 ATV.dial :air3 @10:30:41.48 AS.reconfigure ASslow1 @10:30:41.55 AS.seek 0.01ml @10:30:44.00 delay 2 seconds @10:30:46.02 ATV.dial :bleach @10:30:46.66 AS.seek 0.81ml @10:33:27.86 delay 3 seconds @10:33:30.88 ATV.dial :air3 @10:33:31.45 delay 3 seconds @10:33:34.54 AS.seek 0.835ml @10:33:39.88 delay 3 seconds @10:33:42.90 ATV.dial :bleach @10:33:43.54 AS.seek 0.82ml @10:33:46.86 delay 3 seconds @10:33:49.89 ATV.dial PCR @10:33:50.30 AS.reconfigure ASconfig @10:33:50.37 AS.seek :empty @10:34:37.86 PCR.program :bleachProfile @10:34:37.94 PCR program should complete in < 2:45 @10:34:37.95 PCR.run 10:00 @10:37:36.77 PCR.off @10:37:36.81 AFV.select :carrierSolution @10:37:36.93 delay 2 seconds @10:37:38.96 AS.seek 1ml @10:38:36.87 delay 1 second @10:38:37.88 AFV.select :holdingCoil @10:38:38.01 delay 1 second @10:38:39.04 AS.reconfigure ASfast @10:38:39.11 AS.seek 0ml @10:39:06.53 delay 3 seconds @10:39:09.55 AFV.select :carrierSolution @10:39:09.68 delay 2 seconds @10:39:11.71 AS.reconfigure ASconfig @10:39:11.80 AS.seek 1ml @10:40:09.58 delay 1 second @10:40:10.59 AFV.select :holdingCoil @10:40:10.72 delay 1 second @10:40:11.75 AS.reconfigure ASfast @10:40:11.82 AS.seek 0ml @10:40:39.31 delay 3 seconds @10:40:42.33 AFV.select :carrierSolution @10:40:42.46 delay 2 seconds @10:40:44.49 AS.reconfigure ASconfig @10:40:44.57 AS.seek 1ml @10:41:42.39 delay 1 second @10:41:43.40 AFV.select :holdingCoil @10:41:43.53 delay 1 second @10:41:44.56 AS.reconfigure ASfast @10:41:44.63 AS.seek 0ml @10:42:12.30 delay 3 seconds @10:42:15.32 AFV.select :carrierSolution @10:42:15.45 delay 2 seconds @10:42:17.48 AS.reconfigure ASconfig @10:42:17.56 AS.seek 1ml @10:43:15.39 delay 1 second @10:43:16.41 AFV.select :holdingCoil @10:43:16.54 delay 1 second @10:43:17.57 AS.reconfigure ASfast @10:43:17.64 AS.seek 0ml @10:43:45.02 delay 3 seconds @10:43:48.04 AFV.select :carrierSolution @10:43:48.15 delay 2 seconds @10:43:50.18 AS.reconfigure ASconfig @10:43:50.26 AS.seek 1ml @10:44:48.08 delay 1 second @10:44:49.09 AFV.select :holdingCoil @10:44:49.22 delay 1 second @10:44:50.25 AS.reconfigure ASnorm @10:44:50.32 AS.seek 0ml @10:45:41.07 Setting up PCR for Rp -- writing /var/log/esp/Rp18sep0910h1400ml.pcr @10:45:41.09 Building PCR Slug from ASV with MM2 mix, PP3 primer @10:45:41.13 Priming with carrier @10:45:41.15 ATV.dial ARV @10:45:41.78 ARV.dial :carrier @10:45:42.16 AS.seek 0.5ml @10:46:07.75 AFV.select :carrierSolution @10:46:07.90 delay 2 seconds @10:46:09.93 AS.reconfigure ASconfig @10:46:10.01 AS.seek 1ml @10:46:39.06 delay 1 second @10:46:40.07 AFV.select :holdingCoil @10:46:40.21 delay 1 second @10:46:41.23 ATV.dial :waste @10:46:42.91 AS.reconfigure ASnorm @10:46:42.99 AS.seek :empty @10:47:33.77 AFV.select :carrierSolution @10:47:33.90 delay 2 seconds @10:47:35.93 AS.reconfigure ASconfig @10:47:36.01 AS.seek 0.5ml @10:48:05.12 delay 1 second @10:48:06.14 AFV.select :holdingCoil @10:48:06.27 Building Slug @10:48:06.28 ATV.dial :ARV @10:48:07.94 delay 2 seconds @10:48:09.96 ARV.dial :air1 @10:48:10.39 delay 2 seconds @10:48:12.48 AS.reconfigure ASslow2 @10:48:12.56 AS.seek 0.506ml @10:48:18.92 delay 2 seconds @10:48:20.95 ARV.dial :MM2 @10:48:22.50 delay 2 seconds @10:48:24.53 Adding MM2 @10:48:24.63 AS.seek 0.524ml @10:48:31.98 -> PC.close @10:48:32.01 Arm::Error in bkha -- Claim Arm, then retry closing Processing Clamp @10:48:43.00 delay 25 seconds @10:49:08.02 ARV.dial :PP3 @10:49:08.45 Adding PP3 @10:49:08.47 delay 2 seconds @10:49:10.56 AS.seek 0.53ml @10:49:13.90 -> PC.loadFlush @10:49:13.92 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @10:49:14.02 Hand.open @10:49:14.36 Elbow.reconfigure TightElbow @10:49:14.44 Elbow.seek FlushPuck::Garage @10:49:14.68 Elbow.reconfigure LooseElbow @10:49:14.82 Forearm.seek :garage @10:49:15.87 Hand.close @10:49:16.76 delay 10 seconds @10:49:16.88 Forearm.seek :retract @10:49:17.96 Elbow.reconfigure TightElbow @10:49:18.03 Elbow.seek PC @10:49:19.42 Elbow.reconfigure LooseElbow @10:49:19.55 Forearm.reconfigure TightForearm @10:49:19.62 Forearm.seek PC @10:49:20.89 PC.seek :closed @10:49:25.04 Processing Clamp onSpring/offSpring for 6/29 samples, on/off torque 1.71 @10:49:25.16 Hand.open @10:49:26.00 Forearm.reconfigure LooseForearm @10:49:26.07 Forearm.seek :retract @10:49:26.79 ARV.dial :air2 @10:49:27.22 adding ARV air @10:49:27.24 delay 2 seconds @10:49:29.33 AS.seek 0.536ml @10:49:35.57 delay 5 seconds @10:49:40.59 ARV.dial :carrier @10:49:41.92 delay 7 seconds @10:49:49.01 AS.seek 0.594ml @10:50:31.29 -> PS.unloadFlush @10:50:31.31 NoMethodError in bkha -- undefined method `unloadFlush' for PS:Syringe @10:50:47.61 delay 5 seconds @10:50:53.25 ATV.dial :ASV @10:50:54.12 delay 2 seconds @10:50:56.21 AS.seek 0.6ml @10:51:02.67 delay 4 seconds @10:51:06.70 ATV.dial ARV @10:51:07.26 delay 2 seconds @10:51:09.28 ARV.dial :carrier @10:51:09.30 delay 2 seconds @10:51:11.40 AS.seek 0.65ml @10:51:14.16 -> PC.loadFlushPuck @10:51:25.51 -> PC.unloadFlushPuck @10:51:25.53 Garaging FlushPuck in Processing Clamp @10:51:25.64 Elbow.reconfigure TightElbow @10:51:25.71 Elbow.seek PC @10:51:25.96 Elbow.reconfigure LooseElbow @10:51:26.10 Forearm.seek PC @10:51:27.08 Hand.close @10:51:27.81 PC.seek :open @10:51:32.03 Forearm.seek :retract @10:51:33.06 Elbow.reconfigure TightElbow @10:51:33.13 Elbow.seek :garage @10:51:34.47 Elbow.reconfigure LooseElbow @10:51:34.61 Forearm.reconfigure TightForearm @10:51:34.69 Forearm.seek :garage @10:51:35.86 Hand.open @10:51:36.74 Forearm.reconfigure LooseForearm @10:51:36.82 Forearm.seek :retract @10:51:52.25 -> CC.loadFlush @10:51:52.27 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:51:52.37 Hand.open @10:51:52.74 Elbow.reconfigure TightElbow @10:51:52.82 Elbow.seek FlushPuck::Garage @10:51:52.97 Elbow.reconfigure LooseElbow @10:51:53.11 Forearm.seek :garage @10:51:54.18 Hand.close @10:51:55.06 Forearm.seek :retract @10:51:56.15 Elbow.reconfigure TightElbow @10:51:56.22 Elbow.seek CC @10:51:59.07 Elbow.reconfigure LooseElbow @10:51:59.21 Forearm.reconfigure TightForearm @10:51:59.28 Forearm.seek CC @10:52:00.62 CC.seek :closed @10:52:01.95 delay 4 seconds @10:52:04.89 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.42 @10:52:05.00 Hand.open @10:52:05.88 Forearm.reconfigure LooseForearm @10:52:05.95 Forearm.seek :retract @10:52:06.02 ATV.dial PCR @10:52:06.66 ASV.dial :waste1 @10:52:06.68 ARV.dial :air1 @10:52:07.11 Positioning Slug by 0.324ml @10:52:07.22 AS.seek 0.326ml @10:52:19.53 -> CC.unloadFlush @10:52:19.55 Garaging FlushPuck in Collection Clamp @10:52:19.66 Elbow.reconfigure TightElbow @10:52:19.73 Elbow.seek CC @10:52:19.99 Elbow.reconfigure LooseElbow @10:52:20.12 Forearm.seek CC @10:52:21.12 Hand.close @10:52:21.93 CC.seek :open @10:52:26.36 Forearm.seek :retract @10:52:27.43 Elbow.reconfigure TightElbow @10:52:27.50 Elbow.seek :garage @10:52:30.54 Elbow.reconfigure LooseElbow @10:52:30.68 Forearm.reconfigure TightForearm @10:52:30.75 Forearm.seek :garage @10:52:31.96 Hand.open @10:52:32.75 Forearm.reconfigure LooseForearm @10:52:32.82 Forearm.seek :retract @10:52:59.58 -> CC.loadFlush @10:52:59.60 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:52:59.70 Hand.open @10:53:00.00 Elbow.reconfigure TightElbow @10:53:00.07 Elbow.seek FlushPuck::Garage @10:53:00.23 Elbow.reconfigure LooseElbow @10:53:00.36 Forearm.seek :garage @10:53:01.43 Hand.close @10:53:02.32 Forearm.seek :retract @10:53:03.38 Elbow.reconfigure TightElbow @10:53:03.45 Elbow.seek CC @10:53:06.41 Elbow.reconfigure LooseElbow @10:53:06.55 Forearm.reconfigure TightForearm @10:53:06.62 Forearm.seek CC @10:53:07.93 CC.seek :closed @10:53:12.24 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.53 @10:53:12.35 Hand.open @10:53:13.12 Forearm.reconfigure LooseForearm @10:53:13.20 Forearm.seek :retract @10:53:51.13 -> CC.unloadFlush @10:53:51.16 Garaging FlushPuck in Collection Clamp @10:53:51.26 Elbow.reconfigure TightElbow @10:53:51.34 Elbow.seek CC @10:53:51.62 Elbow.reconfigure LooseElbow @10:53:51.77 Forearm.seek CC @10:53:52.75 Hand.close @10:53:53.56 CC.seek :open @10:53:57.96 Forearm.seek :retract @10:53:58.96 Elbow.reconfigure TightElbow @10:53:59.03 Elbow.seek :garage @10:54:02.20 Elbow.reconfigure LooseElbow @10:54:02.33 Forearm.reconfigure TightForearm @10:54:02.40 Forearm.seek :garage @10:54:03.56 Hand.open @10:54:04.38 Forearm.reconfigure LooseForearm @10:54:04.45 Forearm.seek :retract @10:54:19.14 -> PC.loadFlushPuck @10:54:19.17 Loading FlushPuck into Processing Clamp from FlushPuck::Garage @10:54:19.27 Hand.open @10:54:19.62 Elbow.reconfigure TightElbow @10:54:19.70 Elbow.seek FlushPuck::Garage @10:54:19.88 Elbow.reconfigure LooseElbow @10:54:20.01 Forearm.seek :garage @10:54:21.11 Hand.close @10:54:22.07 Forearm.seek :retract @10:54:23.17 Elbow.reconfigure TightElbow @10:54:23.26 Elbow.seek PC @10:54:24.63 Elbow.reconfigure LooseElbow @10:54:24.76 Forearm.reconfigure TightForearm @10:54:24.84 Forearm.seek PC @10:54:26.20 PC.seek :closed @10:54:30.37 Processing Clamp onSpring/offSpring for 6/29 samples, on/off torque 1.82 @10:54:30.47 Hand.open @10:54:31.24 Forearm.reconfigure LooseForearm @10:54:31.31 Forearm.seek :retract @10:55:59.79 -> PC.unloadFlushPuck @10:55:59.82 Garaging FlushPuck in Processing Clamp @10:55:59.93 Elbow.reconfigure TightElbow @10:56:00.00 Elbow.seek PC @10:56:00.15 Elbow.reconfigure LooseElbow @10:56:00.28 Forearm.seek PC @10:56:01.83 Hand.close @10:56:02.69 PC.seek :open @10:56:06.97 Forearm.seek :retract @10:56:07.97 Elbow.reconfigure TightElbow @10:56:08.04 Elbow.seek :garage @10:56:09.36 Elbow.reconfigure LooseElbow @10:56:09.50 Forearm.reconfigure TightForearm @10:56:09.57 Forearm.seek :garage @10:56:10.75 Hand.open @10:56:11.63 Forearm.reconfigure LooseForearm @10:56:11.70 Forearm.seek :retract @10:57:33.24 delay 5 seconds @10:57:38.27 ATV.dial ARV @10:57:39.17 ASV.dial :PCRlock @10:57:39.58 delay 5 seconds @10:57:44.61 Running Rp Profile60C300 thermocycling @10:57:44.62 PCR.program Profile60C300 @10:57:44.79 Profile60C300 should complete in < 1:45:21 @10:57:44.80 PCR.run 2:16:41.25 @10:58:59.44 -> SSV.air @10:58:59.46 SSV.dial :air @10:59:32.31 -> SS.fill @10:59:32.34 SS.reconfigure SSconfig @10:59:32.43 SS.seek 25ml @10:59:50.33 -> SSV.legend @11:00:14.24 -> SS.empty @11:00:14.26 SS.seek :empty @11:00:42.98 -> CS.air @11:00:43.00 NoMethodError in bkha -- undefined method `air' for CS:Syringe @11:00:50.84 -> CSV.air @11:00:50.86 CSV.dial :air @11:00:59.29 -> CS.fill @11:00:59.32 CS.reconfigure CSconfig @11:00:59.41 CS.seek 10ml @11:01:51.29 -> CS.waste @11:01:51.31 NoMethodError in bkha -- undefined method `waste' for CS:Syringe @11:01:56.07 -> CS.waste1 @11:01:56.09 NoMethodError in bkha -- undefined method `waste1' for CS:Syringe @11:02:04.46 -> CSV.waste1 @11:02:04.48 CSV.dial :waste1 @11:02:24.60 -> CS.empty @11:02:24.62 CS.seek :empty @11:02:31.47 CS.hold @11:02:32.33 UserAbort in bkha -- Collection Syringe seek to empty Aborted! @11:02:49.93 -> CSV.air1 @11:02:49.95 NoMethodError in bkha -- undefined method `air1' for CSV:Shaft @11:03:02.67 -> CS.fill @11:03:02.70 CS.seek 10ml @11:14:36.45 -> CSV @11:14:40.19 -> CSV.air @11:14:40.21 CSV.dial :air @11:14:51.86 -> CS.empty @11:14:51.90 CS.seek :empty @11:15:23.76 -> CS.fill @11:15:23.79 CS.seek 10ml @11:16:06.62 -> PSV.air @11:16:06.63 PSV.dial :air @11:16:16.07 -> PS.fill @11:16:16.11 PS.reconfigure PSconfig @11:16:16.19 PS.seek 2.5ml @11:17:07.00 -> PS.empty @11:17:07.02 PS.seek :empty @11:18:46.60 -> CC.close @11:18:46.65 Arm::Error in bkha -- Claim Arm, then retry closing Collection Clamp @11:19:29.63 -> CTV @11:36:22.22 -> CSV.air @11:36:22.24 CSV.dial :air @11:36:27.67 -> CTV.air @11:36:27.70 CTV.dial :air @11:39:01.70 -> Intake @11:50:31.36 -> CTV @11:53:41.03 -> SSV @11:54:57.09 -> exhaust.open @11:54:57.11 NameError in bkha -- undefined local variable or method `exhaust' for # @11:55:03.76 -> Exhaust.open @11:55:03.78 Exhaust.select :open @11:55:55.74 -> SSV.exhaust @11:55:55.76 SSV.dial :exhaust @11:56:05.92 -> SS.fill @11:56:05.96 SS.seek 25ml @11:56:32.05 -> SSV.empty @11:56:32.08 NoMethodError in bkha -- undefined method `empty' for SSV:Shaft @11:56:36.00 -> SS.empty @11:56:36.03 SS.seek :empty @11:57:18.20 -> SSV.air @11:57:18.22 SSV.dial :air @11:57:24.16 -> Exhaust.close @11:57:24.18 Exhaust.select :close @12:22:34.76 PCR.off @12:22:36.39 PCR for Rp completed -- closed /var/log/esp/Rp18sep0910h1400ml.pcr @12:22:36.48 Cleaning PCR @12:22:36.50 AFV.select :carrierSolution @12:22:36.64 delay 2 seconds @12:22:38.67 AS.reconfigure ASconfig @12:22:38.74 AS.seek 0.5ml @12:22:49.06 delay 1 second @12:22:50.08 AFV.select :holdingCoil @12:22:50.21 ASV.dial :waste1 @12:22:50.60 ATV.dial :air3 @12:22:51.91 AS.reconfigure ASslow1 @12:22:51.98 AS.seek 0.51ml @12:22:54.29 delay 2 seconds @12:22:56.30 ATV.dial :bleach @12:22:56.95 AS.seek 0.81ml @12:23:57.57 delay 3 seconds @12:24:00.59 ATV.dial :air3 @12:24:01.15 delay 3 seconds @12:24:04.23 AS.seek 0.835ml @12:24:09.53 delay 3 seconds @12:24:12.55 ATV.dial :bleach @12:24:13.19 AS.seek 0.82ml @12:24:16.67 delay 3 seconds @12:24:19.69 ARV.dial :waste @12:24:20.97 delay 3 seconds @12:24:23.99 ATV.dial :ARV @12:24:24.90 AS.reconfigure ASconfig @12:24:24.98 AS.seek 0.47ml @12:24:45.41 AFV.select :carrierSolution @12:24:45.55 delay 2 seconds @12:24:47.58 AS.seek 1ml @12:25:18.44 delay 1 second @12:25:19.46 AFV.select :holdingCoil @12:25:19.59 delay 1 second @12:25:20.62 AS.reconfigure ASfast @12:25:20.69 AS.seek 0ml @12:25:48.14 AFV.select :carrierSolution @12:25:48.28 delay 2 seconds @12:25:50.31 AS.reconfigure ASconfig @12:25:50.38 AS.seek 1ml @12:26:48.20 delay 1 second @12:26:49.21 AFV.select :holdingCoil @12:26:49.34 delay 1 second @12:26:50.37 AS.reconfigure ASfast @12:26:50.44 AS.seek 0ml @12:27:17.84 AFV.select :carrierSolution @12:27:17.98 delay 2 seconds @12:27:20.01 AS.reconfigure ASconfig @12:27:20.08 AS.seek 1ml @12:28:17.88 delay 1 second @12:28:18.89 AFV.select :holdingCoil @12:28:19.02 delay 1 second @12:28:20.05 AS.reconfigure ASfast @12:28:20.12 AS.seek 0ml @12:28:47.51 ARV.dial between carrier and air1 @12:28:48.06 ATV.dial :air3 @12:28:49.36 AS.reconfigure ASslow1 @12:28:49.43 AS.seek 0.01ml @12:28:51.78 delay 2 seconds @12:28:53.80 ATV.dial :bleach @12:28:54.45 AS.seek 0.81ml @12:31:35.62 delay 3 seconds @12:31:38.64 ATV.dial :air3 @12:31:39.20 delay 3 seconds @12:31:42.29 AS.seek 0.835ml @12:31:47.62 delay 3 seconds @12:31:50.64 ATV.dial :bleach @12:31:51.28 AS.seek 0.82ml @12:31:54.60 delay 3 seconds @12:31:57.62 ATV.dial PCR @12:31:58.04 AS.reconfigure ASconfig @12:31:58.11 AS.seek :empty @12:32:45.65 PCR.program :bleachProfile @12:32:45.73 PCR program should complete in < 2:45 @12:32:45.74 PCR.run 10:00 @12:35:38.50 PCR.off @12:35:38.54 AFV.select :carrierSolution @12:35:38.67 delay 2 seconds @12:35:40.70 AS.seek 1ml @12:36:38.55 delay 1 second @12:36:39.57 AFV.select :holdingCoil @12:36:39.68 delay 1 second @12:36:40.71 AS.reconfigure ASfast @12:36:40.78 AS.seek 0ml @12:37:08.15 delay 3 seconds @12:37:11.17 AFV.select :carrierSolution @12:37:11.30 delay 2 seconds @12:37:13.33 AS.reconfigure ASconfig @12:37:13.40 AS.seek 1ml @12:38:11.20 delay 1 second @12:38:12.21 AFV.select :holdingCoil @12:38:12.34 delay 1 second @12:38:13.37 AS.reconfigure ASfast @12:38:13.44 AS.seek 0ml @12:38:40.83 delay 3 seconds @12:38:43.85 AFV.select :carrierSolution @12:38:43.98 delay 2 seconds @12:38:46.01 AS.reconfigure ASconfig @12:38:46.10 AS.seek 1ml @12:39:43.90 delay 1 second @12:39:44.91 AFV.select :holdingCoil @12:39:45.04 delay 1 second @12:39:46.07 AS.reconfigure ASfast @12:39:46.14 AS.seek 0ml @12:40:13.52 delay 3 seconds @12:40:16.54 AFV.select :carrierSolution @12:40:16.67 delay 2 seconds @12:40:18.71 AS.reconfigure ASconfig @12:40:18.79 AS.seek 1ml @12:41:16.59 delay 1 second @12:41:17.60 AFV.select :holdingCoil @12:41:17.73 delay 1 second @12:41:18.76 AS.reconfigure ASfast @12:41:18.83 AS.seek 0ml @12:41:46.24 delay 3 seconds @12:41:49.26 AFV.select :carrierSolution @12:41:49.39 delay 2 seconds @12:41:51.42 AS.reconfigure ASconfig @12:41:51.50 AS.seek 1ml @12:42:49.30 delay 1 second @12:42:50.31 AFV.select :holdingCoil @12:42:50.44 delay 1 second @12:42:51.47 AS.reconfigure ASnorm @12:42:51.53 AS.seek 0ml @12:43:42.32 Setting up PCR for Ss -- writing /var/log/esp/Ss18sep0912h1400ml.pcr @12:43:42.34 Building PCR Slug from ASV with MM1 mix, PP2 primer @12:43:42.37 Priming with carrier @12:43:42.39 ATV.dial ARV @12:43:43.66 ARV.dial :carrier @12:43:44.05 AS.seek 0.5ml @12:44:09.62 AFV.select :carrierSolution @12:44:09.77 delay 2 seconds @12:44:11.80 AS.reconfigure ASconfig @12:44:11.88 AS.seek 1ml @12:44:40.90 delay 1 second @12:44:41.92 AFV.select :holdingCoil @12:44:42.05 delay 1 second @12:44:43.07 ATV.dial :waste @12:44:44.74 AS.reconfigure ASnorm @12:44:44.82 AS.seek :empty @12:45:35.61 AFV.select :carrierSolution @12:45:35.75 delay 2 seconds @12:45:37.78 AS.reconfigure ASconfig @12:45:37.86 AS.seek 0.5ml @12:46:06.99 delay 1 second @12:46:08.01 AFV.select :holdingCoil @12:46:08.14 Building Slug @12:46:08.15 ATV.dial :ARV @12:46:09.80 delay 2 seconds @12:46:11.82 ARV.dial :air1 @12:46:12.24 delay 2 seconds @12:46:14.33 AS.reconfigure ASslow2 @12:46:14.40 AS.seek 0.506ml @12:46:20.99 delay 2 seconds @12:46:23.02 ARV.dial :MM1 @12:46:23.65 delay 2 seconds @12:46:25.68 Adding MM1 @12:46:25.77 AS.seek 0.524ml @12:46:44.10 delay 25 seconds @12:47:09.12 ARV.dial :PP2 @12:47:09.54 Adding PP2 @12:47:09.55 delay 2 seconds @12:47:11.66 AS.seek 0.53ml @12:47:18.23 delay 10 seconds @12:47:28.25 ARV.dial :air2 @12:47:28.68 adding ARV air @12:47:28.70 delay 2 seconds @12:47:30.79 AS.seek 0.536ml @12:47:37.04 delay 5 seconds @12:47:42.07 ARV.dial :carrier @12:47:43.40 delay 7 seconds @12:47:50.50 AS.seek 0.594ml @12:48:49.09 delay 5 seconds @12:48:54.11 ATV.dial :ASV @12:48:54.52 delay 2 seconds @12:48:56.61 AS.seek 0.6ml @12:49:03.16 delay 4 seconds @12:49:07.18 ATV.dial ARV @12:49:07.74 delay 2 seconds @12:49:09.76 ARV.dial :carrier @12:49:09.78 delay 2 seconds @12:49:11.89 AS.seek 0.65ml @12:50:02.60 delay 4 seconds @12:50:06.62 ATV.dial PCR @12:50:07.26 ASV.dial :waste1 @12:50:07.28 ARV.dial :air1 @12:50:07.81 Positioning Slug by 0.324ml @12:50:07.92 AS.seek 0.326ml @12:55:34.18 delay 5 seconds @12:55:39.20 ATV.dial ARV @12:55:40.10 ASV.dial :PCRlock @12:55:40.60 delay 5 seconds @12:55:45.63 Running Ss Profile60C350 thermocycling @12:55:45.65 PCR.program Profile60C350 @12:55:45.82 Profile60C350 should complete in < 1:48:05 @12:55:45.85 PCR.run 2:20:06.25 @13:31:41.64 Session Ended @14:12:55.74 Session Begun @14:21:17.15 PCR.off @14:21:19.28 PCR for Ss completed -- closed /var/log/esp/Ss18sep0912h1400ml.pcr @14:21:19.38 Cleaning PCR @14:21:19.39 AFV.select :carrierSolution @14:21:19.53 delay 2 seconds @14:21:21.56 AS.reconfigure ASconfig @14:21:21.63 AS.seek 0.5ml @14:21:31.94 delay 1 second @14:21:32.95 AFV.select :holdingCoil @14:21:33.08 ASV.dial :waste1 @14:21:33.49 ATV.dial :air3 @14:21:35.37 AS.reconfigure ASslow1 @14:21:35.45 AS.seek 0.51ml @14:21:37.77 delay 2 seconds @14:21:39.79 ATV.dial :bleach @14:21:40.45 AS.seek 0.81ml @14:21:49.11 Session Begun @14:21:52.76 -> showlog 100 @14:22:40.80 -> abort! "Resetting ESP and restarting" @14:22:40.82 CmdServer::Client::Error in bkha -- Aborting Mission: Resetting ESP and restarting @14:22:40.88
AS.stop @14:22:41.00 BadNews.email "Resetting ESP and restarting",Subject: "18tun1 ABORTED!" @14:22:47.75 Session Ended @14:22:47.80 Session Ended @17:46:06.03CEST09-Sep-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @17:46:08.57 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @17:46:08.86 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @17:46:14.92 Polling Can every 10:00 @17:46:16.81 SPE.coast @17:46:16.86 SPE.configure SPEconfig @17:46:16.96 AS.reconfigure ASconfig @17:46:17.01 AS.hold @17:46:17.07 AS.configure ASconfig @17:46:17.17 SS.reconfigure SSconfig @17:46:17.22 SS.hold @17:46:17.27 SS.configure SSconfig @17:46:17.37 CC.reconfigure CCconfig @17:46:17.41 CC.hold @17:46:17.47 CC.configure CCconfig @17:46:17.57 CS.reconfigure CSconfig @17:46:17.61 CS.hold @17:46:17.69 CS.configure CSconfig @17:46:18.16 PC.reconfigure PCconfig @17:46:18.21 PC.hold @17:46:18.28 PC.configure PCconfig @17:46:18.38 PS.reconfigure PSconfig @17:46:18.42 PS.hold @17:46:18.50 PS.configure PSconfig @17:46:18.62 Forearm.reconfigure LooseForearm @17:46:18.67 Forearm.hold @17:46:18.73 Forearm.configure LooseForearm @17:46:18.83 Elbow.reconfigure LooseElbow @17:46:18.87 Elbow.hold @17:46:18.93 Elbow.configure LooseElbow @17:46:19.05 SC.reconfigure SCloose @17:46:19.09 SC.hold @17:46:19.15 SC.configure SCloose @17:46:19.24 SE.reconfigure SEconfig @17:46:19.29 SE.hold @17:46:19.35 SE.configure SEconfig @17:46:19.40 CH.coast @17:46:19.45 CH.configure CHconfig @17:46:19.48 PH.coast @17:46:19.54 PH.configure PHconfig @17:46:19.58 PCR.off @17:46:19.61 PCR.pidtemp= 50,0.1,0,300,25,25 @17:46:19.64 PCR.caltemp= 2.7073,0.0373,0 @17:46:19.66 All dwarf controllers running firmware version 3.75 @17:46:19.81 Loading /home/esp/esp2/mission/18tun1.rb mission @17:46:19.87 Preparing to sleep until +0:00:15 @17:46:19.90 GoodNews.email "Starting 18tun1 w/pucks in tube 2\n{}\nCan@17:46:14, 24.3C, 49% humidity, 14.6psia, 11.928V, 0.985A, 0.979A avg, 11.75W",Subject: "18tun1 begun" @17:46:19.98 AS.coast @17:46:20.02
Asleep til +0:00:15 @17:46:20.06 Continuing at 17:46 @17:46:20.10 SS.coast @17:46:20.16 CC.coast @17:46:20.21 CS.coast @17:46:20.26 PC.coast @17:46:20.29 PS.coast @17:46:20.33 Forearm.coast @17:46:20.37 Elbow.coast @17:46:20.41 SC.coast @17:46:20.44 SE.coast @17:46:20.84 Gate.power :core,:OFF @17:46:20.85 Gate.power :raw,:OFF @17:46:20.86 Shutdown @17:46:25.92 Session Begun @17:46:28.61 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:105\n{}\nCan@17:46:20, 24.3C, 49% humidity, 14.6psia, 11.933V, 0.979A, 0.979A avg, 11.68W",Subject: "Asleep until 17:46" @17:46:34.87
Powering core bus @17:46:39.68 Gate.power :raw,:ON @17:46:40.38 SPE.coast @17:46:40.47 SPE.configure SPEconfig @17:46:40.59 AS.configure ASconfig @17:46:40.72 SS.configure SSconfig @17:46:40.84 CC.configure CCconfig @17:46:40.99 CS.configure CSconfig @17:46:41.14 PC.configure PCconfig @17:46:41.26 PS.configure PSconfig @17:46:41.44 Forearm.configure LooseForearm @17:46:41.57 Elbow.configure LooseElbow @17:46:41.72 SC.configure SCloose @17:46:41.84 SE.configure SEconfig @17:46:41.91 CH.coast @17:46:41.98 CH.configure CHconfig @17:46:42.04 PH.coast @17:46:42.12 PH.configure PHconfig @17:46:42.18 PCR.off @17:46:42.23 PCR.pidtemp= 50,0.1,0,300,25,25 @17:46:42.27 PCR.caltemp= 2.7073,0.0373,0 @17:46:42.31 All dwarf controllers running firmware version 3.75 @17:46:42.45 Valve.reset @17:46:42.64 PCR.off @17:46:42.69 PCR.pidtemp= 50,0.1,0,300,25,25 @17:46:42.73 PCR.caltemp= 2.7073,0.0373,0 @17:46:42.97 Intake.select :close @17:46:42.98 Exhaust.select :close @17:46:43.01 CTV.dial :air @17:46:43.13 PSV.dial :air @17:46:43.17 CSV.dial :waste1 @17:46:43.36
Forearm.reconfigure LooseForearm @17:46:43.90 Forearm.home.seek :retract @17:46:44.00 SSV.dial :spit,avoiding: :exhaust @17:46:44.02 PS.reconfigure PSconfig @17:46:44.14 PS.home.seek 0ml @17:46:44.31 CS.reconfigure CSfast @17:46:44.35 SS.reconfigure SSconfig @17:46:44.51 CS.home.seek 0ml @17:46:44.55 SS.home.seek 0ml @17:46:44.69 Hand.close @17:46:44.73
Elbow.jog 1500 @17:46:45.39 Elbow.reconfigure LooseElbow @17:46:45.47 Elbow.home.seek :clear @17:46:48.88 SE.reconfigure SEconfig @17:46:48.95 SE.home.seek :down @17:46:49.64 SC.reconfigure SCsloppy @17:46:49.72 SC.home.seek :initial @17:46:53.42 PS.coast @17:46:56.58 -> showlog 100 @17:47:00.21
Forearm.seek :retract @17:47:00.31 Elbow.seek :hammer @17:47:01.48 Forearm.reconfigure TightForearm @17:47:01.56 Forearm.seek 5 + 350 counts @17:47:02.63 Forearm.seek :retract @17:47:03.89 PC.reconfigure PCconfig @17:47:03.91
CC.reconfigure CCconfig @17:47:04.02 PC.home.seek :open @17:47:04.06
CC.home.seek :open @17:47:07.43 Phase [mbaPhase] @17:47:07.45 GoodNews.email "At 17:47:07 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:105\n{}\nCan@17:47:07, 24.3C, 48% humidity, 14.6psia, 11.992V, 0.739A, 0.739A avg, 8.86W",Subject: "Started 18tun1 phase mbaPhase" @17:47:07.49CEST09-Sep-18
==================> STARTED MBAsuperphase (phase #1) <=== @17:47:07.62 Starting mfb @17:47:07.63 pcrBAC sampling at most 1500ml @17:47:07.76 Loading sh1 puck from tube 2 into Collection Clamp @17:47:07.88 Readying the MFB @17:47:07.90
Hand.open @17:47:07.93 Homing MFB Syringe @17:47:08.02 AFV.select :holdingCoil @17:47:08.20 ATV.dial :air2 @17:47:09.04
SC.reconfigure SCloose @17:47:09.23 Elbow.reconfigure TightElbow @17:47:09.31 Elbow.seek 2 @17:47:09.73 AS.reconfigure ASconfig @17:47:09.90 AS.home.seek :home @17:47:10.07
Forearm.seek 2 + 700 counts @17:47:11.16 SE.reconfigure SEpick @17:47:11.29 SE.seek beamBreak @17:47:13.76 BACfans.email "Started mfb in tube 2 at 17:47:07CEST09-Sep-18\n{}\nCan@17:47:07, 24.3C, 48% humidity, 14.6psia, 11.992V, 0.739A, 0.739A avg, 8.86W",Subject: "pcrBAC sampling at most 1500ml" @17:47:14.39
SE.configure SEconfig @17:47:14.63 Elbow.seek 2 @17:47:14.89 Forearm.seek 2 + 300 counts @17:47:15.09 Forearm.reconfigure LooseForearm @17:47:15.16 Hand.close @17:47:15.91 SE.seek :down @17:47:16.19
Forearm.seek :retract @17:47:16.64 AFV.select :carrierSolution @17:47:16.79 delay 2 seconds @17:47:17.18
Elbow.seek CC @17:47:18.91 SE.coast @17:47:18.93
Elbow.reconfigure LooseElbow @17:47:19.08 AS.seek 1ml @17:47:19.20
Forearm.reconfigure TightForearm @17:47:19.27 Forearm.seek CC @17:47:20.61 CC.seek :closed @17:47:24.95 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.57 @17:47:24.96 Hand.open @17:47:25.82 Forearm.reconfigure LooseForearm @17:47:25.89 Forearm.seek :retract @17:47:26.94 SC.coast @17:47:27.00 collectSample 1500ml w/bubble point of 55psi @17:47:27.01 Priming 7ml @17:47:27.03 Gate.power :sampler,:ON @17:47:27.07 Intake.select :close @17:47:27.09 Exhaust.select :close @17:47:27.12 SS.hold @17:47:27.40 Calibrating pressure and temperature sensors @17:47:27.42 CTV.dial :air @17:47:27.45 SSV.dial :spit,avoiding: :exhaust @17:47:27.50 SS.reconfigure SSfast @17:47:27.57 SS.seek 5ml @17:47:30.66 SSV.dial CBV @17:47:30.69
CBV.dial SSV @17:47:32.22 delay 3.8 seconds @17:47:36.16 SSV.dial :spit @17:47:37.70 SS.seek 0ml @17:47:40.32 SSV.dial CBV @17:47:41.81 CTV.dial :intake @17:47:42.69 Waiting for external sampler to finish priming... @17:47:42.70 delayUntil "17:52:27.06" @17:48:11.99 delay 1 second @17:48:13.01 AFV.select :holdingCoil @17:48:13.13 delay 1 second @17:48:14.16 ATV.dial :waste @17:48:15.23 AS.reconfigure ASnorm @17:48:15.30 AS.home.seek :empty @17:49:07.88 ATV.dial :waste @17:49:07.90 ARV.dial between carrier and air1 @17:49:08.03 ASV.dial :waste1 @17:52:27.09 SSV.dial CBV @17:52:27.12
CBV.dial SSV @17:52:27.15 Allowing 40s for 7.0ml of remaining 7.0ml @17:52:27.18 Intake.select :open @17:52:29.39 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:52:29.41 SS.reconfigure SSinhale.with maxSpeed: 150 @17:52:29.50 SS.seek 7ml @17:52:35.80 Stroke completed -- waiting for pressure to equalize ... @17:52:49.09 Intake.select :close @17:52:49.28 SSV.dial :exhaust @17:52:50.14 Exhaust.select :open @17:52:50.27 SS.reconfigure SSexhale @17:52:50.35 SS.seek 0ml @17:52:54.60 Exhaust.select :close @17:52:54.75 Sampling 1500.0ml @17:52:54.78 Intake.select :close @17:52:54.80 Exhaust.select :close @17:52:54.83 SS.reconfigure SSconfig @17:52:54.91 SS.hold @17:52:55.15 CTV.dial :intake @17:52:55.17 SSV.dial CBV @17:52:55.21
CBV.dial SSV @17:52:56.11 Allowing 130s for 25.0ml of remaining 1500.0ml @17:52:56.12 Intake.select :open @17:52:58.33 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:52:58.36 SS.reconfigure SSinhale.with maxSpeed: 150 @17:52:58.45 SS.seek 25ml @17:53:02.13 Vacuum too high -- waiting up to 40s @17:53:07.23 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:53:07.25 SS.reconfigure SSinhale.with maxSpeed: 135 @17:53:07.34 SS.seek 25ml @17:53:10.10 Vacuum too high -- waiting up to 40s @17:53:15.24 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:53:15.26 SS.reconfigure SSinhale.with maxSpeed: 122 @17:53:15.35 SS.seek 25ml @17:53:18.26 Vacuum too high -- waiting up to 40s @17:53:23.90 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:53:23.93 SS.reconfigure SSinhale.with maxSpeed: 110 @17:53:24.02 SS.seek 25ml @17:53:27.51 Vacuum too high -- waiting up to 40s @17:53:32.37 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:53:32.39 SS.reconfigure SSinhale.with maxSpeed: 99 @17:53:32.48 SS.seek 25ml @17:53:35.95 Vacuum too high -- waiting up to 40s @17:53:40.57 SS.reconfigure SSinhale.with maxSpeed: 89 @17:53:40.65 SS.seek 25ml @17:53:44.38 Vacuum too high -- waiting up to 40s @17:53:49.46 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:53:49.48 SS.reconfigure SSinhale.with maxSpeed: 80 @17:53:49.57 SS.seek 25ml @17:53:51.32 Stroke completed -- waiting for pressure to equalize ... @17:53:53.92 Intake.select :close @17:53:54.10 SSV.dial :exhaust @17:53:54.98 Exhaust.select :open @17:53:55.12 SS.reconfigure SSexhale @17:53:55.20 SS.seek 0ml @17:54:04.81 Exhaust.select :close @17:54:04.94 SSV.dial CBV @17:54:04.97
CBV.dial SSV @17:54:05.86 Allowing 130s for 25.0ml of remaining 1475.0ml @17:54:05.87 Intake.select :open @17:54:08.08 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:54:08.10 SS.reconfigure SSinhale.with maxSpeed: 80 @17:54:08.20 SS.seek 25ml @17:54:12.51 Vacuum too high -- waiting up to 40s @17:54:16.35 Intake @15.5psia, vacuum from 9.0 to 13.0psi @17:54:16.37 SS.reconfigure SSinhale.with maxSpeed: 72 @17:54:16.46 SS.seek 25ml @17:54:20.70 Vacuum too high -- waiting up to 40s @17:54:24.61 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:54:24.64 SS.reconfigure SSinhale.with maxSpeed: 65 @17:54:24.72 SS.seek 25ml @17:54:29.82 Vacuum too high -- waiting up to 40s @17:54:33.64 SS.reconfigure SSinhale.with maxSpeed: 59 @17:54:33.71 SS.seek 25ml @17:54:40.38 Vacuum too high -- waiting up to 40s @17:54:44.21 Intake @15.5psia, vacuum from 9.0 to 13.0psi @17:54:44.24 SS.reconfigure SSinhale.with maxSpeed: 53 @17:54:44.33 SS.seek 25ml @17:54:51.80 Vacuum too high -- waiting up to 40s @17:54:55.91 SS.reconfigure SSinhale.with maxSpeed: 48 @17:54:55.98 SS.seek 25ml @17:55:00.50 Stroke completed -- waiting for pressure to equalize ... @17:55:04.36 Intake.select :close @17:55:04.54 SSV.dial :exhaust @17:55:05.42 Exhaust.select :open @17:55:05.54 SS.reconfigure SSexhale @17:55:05.61 SS.seek 0ml @17:55:15.47 Exhaust.select :close @17:55:15.61 SSV.dial CBV @17:55:15.64
CBV.dial SSV @17:55:16.54 Allowing 130s for 25.0ml of remaining 1450.0ml @17:55:16.55 Intake.select :open @17:55:18.78 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:55:18.80 SS.reconfigure SSinhale.with maxSpeed: 48 @17:55:18.88 SS.seek 25ml @17:55:28.60 Vacuum too high -- waiting up to 40s @17:55:31.91 Intake @15.5psia, vacuum from 9.0 to 13.0psi @17:55:31.93 SS.reconfigure SSinhale.with maxSpeed: 43 @17:55:32.02 SS.seek 25ml @17:56:02.80 Stroke completed -- waiting for pressure to equalize ... @17:56:07.29 Intake.select :close @17:56:07.46 SSV.dial :exhaust @17:56:08.33 Exhaust.select :open @17:56:08.47 SS.reconfigure SSexhale @17:56:08.54 SS.seek 0ml @17:56:18.22 Exhaust.select :close @17:56:18.36 SSV.dial CBV @17:56:18.39
CBV.dial SSV @17:56:19.29 Allowing 130s for 25.0ml of remaining 1425.0ml @17:56:19.30 Intake.select :open @17:56:21.51 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:56:21.53 SS.reconfigure SSinhale.with maxSpeed: 43 @17:56:21.63 SS.seek 25ml @17:56:37.44 Vacuum too high -- waiting up to 40s @17:56:40.79 Intake @15.4psia, vacuum from 8.9 to 12.9psi @17:56:40.81 SS.reconfigure SSinhale.with maxSpeed: 39 @17:56:40.90 SS.seek 25ml @17:57:09.63 Stroke completed -- waiting for pressure to equalize ... @17:57:12.86 Intake.select :close @17:57:13.04 SSV.dial :exhaust @17:57:13.93 Exhaust.select :open @17:57:14.06 SS.reconfigure SSexhale @17:57:14.14 SS.seek 0ml @17:57:23.42 Exhaust.select :close @17:57:23.56 SSV.dial CBV @17:57:23.59
CBV.dial SSV @17:57:24.47 Allowing 130s for 25.0ml of remaining 1400.0ml @17:57:24.49 Intake.select :open @17:57:26.71 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:57:26.73 SS.reconfigure SSinhale.with maxSpeed: 39 @17:57:26.82 SS.seek 25ml @17:58:13.13 Stroke completed -- waiting for pressure to equalize ... @17:58:16.98 Intake.select :close @17:58:17.15 SSV.dial :exhaust @17:58:18.03 Exhaust.select :open @17:58:18.16 SS.reconfigure SSexhale @17:58:18.25 SS.seek 0ml @17:58:28.02 Exhaust.select :close @17:58:28.17 SSV.dial CBV @17:58:28.20
CBV.dial SSV @17:58:29.10 Allowing 130s for 25.0ml of remaining 1375.0ml @17:58:29.11 Intake.select :open @17:58:31.32 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:58:31.34 SS.reconfigure SSinhale.with maxSpeed: 39 @17:58:31.43 SS.seek 25ml @17:59:17.30 Stroke completed -- waiting for pressure to equalize ... @17:59:20.98 Intake.select :close @17:59:21.16 SSV.dial :exhaust @17:59:22.04 Exhaust.select :open @17:59:22.17 SS.reconfigure SSexhale @17:59:22.25 SS.seek 0ml @17:59:31.92 Exhaust.select :close @17:59:32.07 SSV.dial CBV @17:59:32.10
CBV.dial SSV @17:59:32.98 Allowing 130s for 25.0ml of remaining 1350.0ml @17:59:32.99 Intake.select :open @17:59:35.20 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:59:35.22 SS.reconfigure SSinhale.with maxSpeed: 39 @17:59:35.31 SS.seek 25ml @18:00:21.23 Stroke completed -- waiting for pressure to equalize ... @18:00:25.07 Intake.select :close @18:00:25.25 SSV.dial :exhaust @18:00:26.12 Exhaust.select :open @18:00:26.25 SS.reconfigure SSexhale @18:00:26.34 SS.seek 0ml @18:00:36.06 Exhaust.select :close @18:00:36.19 SSV.dial CBV @18:00:36.22
CBV.dial SSV @18:00:37.13 Allowing 130s for 25.0ml of remaining 1325.0ml @18:00:37.14 Intake.select :open @18:00:39.35 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:00:39.37 SS.reconfigure SSinhale.with maxSpeed: 39 @18:00:39.47 SS.seek 25ml @18:01:25.47 Stroke completed -- waiting for pressure to equalize ... @18:01:29.31 Intake.select :close @18:01:29.48 SSV.dial :exhaust @18:01:30.36 Exhaust.select :open @18:01:30.49 SS.reconfigure SSexhale @18:01:30.58 SS.seek 0ml @18:01:40.34 Exhaust.select :close @18:01:40.48 SSV.dial CBV @18:01:40.51
CBV.dial SSV @18:01:41.39 Allowing 130s for 25.0ml of remaining 1300.0ml @18:01:41.41 Intake.select :open @18:01:43.62 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:01:43.65 SS.reconfigure SSinhale.with maxSpeed: 39 @18:01:43.74 SS.seek 25ml @18:02:29.33 Stroke completed -- waiting for pressure to equalize ... @18:02:32.56 Intake.select :close @18:02:32.73 SSV.dial :exhaust @18:02:33.60 Exhaust.select :open @18:02:33.73 SS.reconfigure SSexhale @18:02:33.80 SS.seek 0ml @18:02:43.64 Exhaust.select :close @18:02:43.79 SSV.dial CBV @18:02:43.82
CBV.dial SSV @18:02:44.73 Allowing 130s for 25.0ml of remaining 1275.0ml @18:02:44.74 Intake.select :open @18:02:46.95 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:02:46.98 SS.reconfigure SSinhale.with maxSpeed: 39 @18:02:47.06 SS.seek 25ml @18:03:32.61 Stroke completed -- waiting for pressure to equalize ... @18:03:35.83 Intake.select :close @18:03:36.00 SSV.dial :exhaust @18:03:36.88 Exhaust.select :open @18:03:37.01 SS.reconfigure SSexhale @18:03:37.09 SS.seek 0ml @18:03:46.95 Exhaust.select :close @18:03:47.09 SSV.dial CBV @18:03:47.12
CBV.dial SSV @18:03:48.01 Allowing 130s for 25.0ml of remaining 1250.0ml @18:03:48.02 Intake.select :open @18:03:50.24 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:03:50.26 SS.reconfigure SSinhale.with maxSpeed: 39 @18:03:50.35 SS.seek 25ml @18:04:35.92 Stroke completed -- waiting for pressure to equalize ... @18:04:38.99 Intake.select :close @18:04:39.17 SSV.dial :exhaust @18:04:40.04 Exhaust.select :open @18:04:40.17 SS.reconfigure SSexhale @18:04:40.25 SS.seek 0ml @18:04:50.09 Exhaust.select :close @18:04:50.23 SSV.dial CBV @18:04:50.26
CBV.dial SSV @18:04:51.16 Allowing 130s for 25.0ml of remaining 1225.0ml @18:04:51.17 Intake.select :open @18:04:53.38 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:04:53.41 SS.reconfigure SSinhale.with maxSpeed: 39 @18:04:53.50 SS.seek 25ml @18:05:39.06 Stroke completed -- waiting for pressure to equalize ... @18:05:42.28 Intake.select :close @18:05:42.44 SSV.dial :exhaust @18:05:43.32 Exhaust.select :open @18:05:43.45 SS.reconfigure SSexhale @18:05:43.53 SS.seek 0ml @18:05:53.20 Exhaust.select :close @18:05:53.35 SSV.dial CBV @18:05:53.38
CBV.dial SSV @18:05:54.26 Allowing 130s for 25.0ml of remaining 1200.0ml @18:05:54.27 Intake.select :open @18:05:56.48 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:05:56.50 SS.reconfigure SSinhale.with maxSpeed: 39 @18:05:56.59 SS.seek 25ml @18:06:42.40 Stroke completed -- waiting for pressure to equalize ... @18:06:46.23 Intake.select :close @18:06:46.42 SSV.dial :exhaust @18:06:47.29 Exhaust.select :open @18:06:47.43 SS.reconfigure SSexhale @18:06:47.52 SS.seek 0ml @18:06:57.23 Exhaust.select :close @18:06:57.37 SSV.dial CBV @18:06:57.40
CBV.dial SSV @18:06:58.30 Allowing 130s for 25.0ml of remaining 1175.0ml @18:06:58.31 Intake.select :open @18:07:00.53 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:07:00.56 SS.reconfigure SSinhale.with maxSpeed: 39 @18:07:00.65 SS.seek 25ml @18:07:07.46 -> exit @18:07:07.48 Session Ended @18:07:46.33
Stroke completed -- waiting for pressure to equalize ... @18:07:49.55 Intake.select :close @18:07:49.71 SSV.dial :exhaust @18:07:50.56 Exhaust.select :open @18:07:50.68 SS.reconfigure SSexhale @18:07:50.73 SS.seek 0ml @18:08:00.25 Exhaust.select :close @18:08:00.37 SSV.dial CBV @18:08:00.40
CBV.dial SSV @18:08:01.27 Allowing 130s for 25.0ml of remaining 1150.0ml @18:08:01.28 Intake.select :open @18:08:03.45 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:08:03.47 SS.reconfigure SSinhale.with maxSpeed: 39 @18:08:03.52 SS.seek 25ml @18:08:49.43 Stroke completed -- waiting for pressure to equalize ... @18:08:53.26 Intake.select :close @18:08:53.41 SSV.dial :exhaust @18:08:54.27 Exhaust.select :open @18:08:54.38 SS.reconfigure SSexhale @18:08:54.43 SS.seek 0ml @18:09:04.03 Exhaust.select :close @18:09:04.15 SSV.dial CBV @18:09:04.18
CBV.dial SSV @18:09:05.04 Allowing 130s for 25.0ml of remaining 1125.0ml @18:09:05.05 Intake.select :open @18:09:07.22 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:09:07.23 SS.reconfigure SSinhale.with maxSpeed: 39 @18:09:07.76 SS.seek 25ml @18:09:53.68 Stroke completed -- waiting for pressure to equalize ... @18:09:56.93 Intake.select :close @18:09:57.09 SSV.dial :exhaust @18:09:57.94 Exhaust.select :open @18:09:58.05 SS.reconfigure SSexhale @18:09:58.10 SS.seek 0ml @18:10:07.72 Exhaust.select :close @18:10:07.84 SSV.dial CBV @18:10:07.87
CBV.dial SSV @18:10:08.74 Allowing 130s for 25.0ml of remaining 1100.0ml @18:10:08.75 Intake.select :open @18:10:10.92 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:10:10.94 SS.reconfigure SSinhale.with maxSpeed: 39 @18:10:10.99 SS.seek 25ml @18:10:57.06 Stroke completed -- waiting for pressure to equalize ... @18:11:00.28 Intake.select :close @18:11:00.44 SSV.dial :exhaust @18:11:01.29 Exhaust.select :open @18:11:01.40 SS.reconfigure SSexhale @18:11:01.46 SS.seek 0ml @18:11:11.18 Exhaust.select :close @18:11:11.30 SSV.dial CBV @18:11:11.33
CBV.dial SSV @18:11:12.19 Allowing 130s for 25.0ml of remaining 1075.0ml @18:11:12.20 Intake.select :open @18:11:14.38 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:11:14.39 SS.reconfigure SSinhale.with maxSpeed: 39 @18:11:14.44 SS.seek 25ml @18:12:00.12 Stroke completed -- waiting for pressure to equalize ... @18:12:03.33 Intake.select :close @18:12:03.49 SSV.dial :exhaust @18:12:04.34 Exhaust.select :open @18:12:04.46 SS.reconfigure SSexhale @18:12:04.52 SS.seek 0ml @18:12:14.20 Exhaust.select :close @18:12:14.32 SSV.dial CBV @18:12:14.35
CBV.dial SSV @18:12:15.24 Allowing 130s for 25.0ml of remaining 1050.0ml @18:12:15.25 Intake.select :open @18:12:17.43 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:12:17.44 SS.reconfigure SSinhale.with maxSpeed: 39 @18:12:17.49 SS.seek 25ml @18:13:03.17 Stroke completed -- waiting for pressure to equalize ... @18:13:06.39 Intake.select :close @18:13:06.55 SSV.dial :exhaust @18:13:07.40 Exhaust.select :open @18:13:07.51 SS.reconfigure SSexhale @18:13:07.56 SS.seek 0ml @18:13:17.12 Exhaust.select :close @18:13:17.24 SSV.dial CBV @18:13:17.27
CBV.dial SSV @18:13:18.17 Allowing 130s for 25.0ml of remaining 1025.0ml @18:13:18.20 Intake.select :open @18:13:20.39 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:13:20.41 SS.reconfigure SSinhale.with maxSpeed: 39 @18:13:20.46 SS.seek 25ml @18:14:06.40 Stroke completed -- waiting for pressure to equalize ... @18:14:09.61 Intake.select :close @18:14:10.23 SSV.dial :exhaust @18:14:11.07 Exhaust.select :open @18:14:11.19 SS.reconfigure SSexhale @18:14:11.24 SS.seek 0ml @18:14:20.90 Exhaust.select :close @18:14:21.02 SSV.dial CBV @18:14:21.05
CBV.dial SSV @18:14:21.91 Allowing 130s for 25.0ml of remaining 1000.0ml @18:14:21.92 Intake.select :open @18:14:24.10 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:14:24.11 SS.reconfigure SSinhale.with maxSpeed: 39 @18:14:24.16 SS.seek 25ml @18:15:10.15 Stroke completed -- waiting for pressure to equalize ... @18:15:14.03 Intake.select :close @18:15:14.19 SSV.dial :exhaust @18:15:15.05 Exhaust.select :open @18:15:15.16 SS.reconfigure SSexhale @18:15:15.22 SS.seek 0ml @18:15:24.93 Exhaust.select :close @18:15:25.05 SSV.dial CBV @18:15:25.08
CBV.dial SSV @18:15:25.94 Allowing 130s for 25.0ml of remaining 975.0ml @18:15:25.95 Intake.select :open @18:15:28.12 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:15:28.14 SS.reconfigure SSinhale.with maxSpeed: 39 @18:15:28.19 SS.seek 25ml @18:16:13.82 Stroke completed -- waiting for pressure to equalize ... @18:16:17.03 Intake.select :close @18:16:17.19 SSV.dial :exhaust @18:16:18.04 Exhaust.select :open @18:16:18.16 SS.reconfigure SSexhale @18:16:18.21 SS.seek 0ml @18:16:27.79 Exhaust.select :close @18:16:27.91 SSV.dial CBV @18:16:27.94
CBV.dial SSV @18:16:28.82 Allowing 130s for 25.0ml of remaining 950.0ml @18:16:28.83 Intake.select :open @18:16:31.00 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:16:31.02 SS.reconfigure SSinhale.with maxSpeed: 39 @18:16:31.07 SS.seek 25ml @18:17:17.06 Stroke completed -- waiting for pressure to equalize ... @18:17:20.92 Intake.select :close @18:17:21.08 SSV.dial :exhaust @18:17:21.93 Exhaust.select :open @18:17:22.04 SS.reconfigure SSexhale @18:17:22.10 SS.seek 0ml @18:17:31.82 Exhaust.select :close @18:17:31.94 SSV.dial CBV @18:17:31.97
CBV.dial SSV @18:17:32.83 Allowing 130s for 25.0ml of remaining 925.0ml @18:17:32.84 Intake.select :open @18:17:35.02 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:17:35.03 SS.reconfigure SSinhale.with maxSpeed: 39 @18:17:35.08 SS.seek 25ml @18:18:20.62 Stroke completed -- waiting for pressure to equalize ... @18:18:23.82 Intake.select :close @18:18:23.97 SSV.dial :exhaust @18:18:25.28 Exhaust.select :open @18:18:25.39 SS.reconfigure SSexhale @18:18:25.44 SS.seek 0ml @18:18:35.01 Exhaust.select :close @18:18:35.13 SSV.dial CBV @18:18:35.16
CBV.dial SSV @18:18:36.05 Allowing 130s for 25.0ml of remaining 900.0ml @18:18:36.06 Intake.select :open @18:18:38.24 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:18:38.26 SS.reconfigure SSinhale.with maxSpeed: 39 @18:18:38.31 SS.seek 25ml @18:19:24.20 Stroke completed -- waiting for pressure to equalize ... @18:19:27.43 Intake.select :close @18:19:27.59 SSV.dial :exhaust @18:19:28.44 Exhaust.select :open @18:19:28.55 SS.reconfigure SSexhale @18:19:28.61 SS.seek 0ml @18:19:38.13 Exhaust.select :close @18:19:38.25 SSV.dial CBV @18:19:38.28
CBV.dial SSV @18:19:39.16 Allowing 130s for 25.0ml of remaining 875.0ml @18:19:39.17 Intake.select :open @18:19:41.34 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:19:41.36 SS.reconfigure SSinhale.with maxSpeed: 39 @18:19:41.41 SS.seek 25ml @18:20:27.41 Stroke completed -- waiting for pressure to equalize ... @18:20:30.63 Intake.select :close @18:20:30.78 SSV.dial :exhaust @18:20:31.64 Exhaust.select :open @18:20:31.75 SS.reconfigure SSexhale @18:20:31.80 SS.seek 0ml @18:20:41.37 Exhaust.select :close @18:20:41.49 SSV.dial CBV @18:20:41.52
CBV.dial SSV @18:20:42.38 Allowing 130s for 25.0ml of remaining 850.0ml @18:20:42.39 Intake.select :open @18:20:44.56 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:20:44.58 SS.reconfigure SSinhale.with maxSpeed: 39 @18:20:44.63 SS.seek 25ml @18:21:30.54 Stroke completed -- waiting for pressure to equalize ... @18:21:34.38 Intake.select :close @18:21:34.54 SSV.dial :exhaust @18:21:35.39 Exhaust.select :open @18:21:35.50 SS.reconfigure SSexhale @18:21:35.56 SS.seek 0ml @18:21:45.13 Exhaust.select :close @18:21:45.25 SSV.dial CBV @18:21:45.28
CBV.dial SSV @18:21:46.16 Allowing 130s for 25.0ml of remaining 825.0ml @18:21:46.17 Intake.select :open @18:21:48.34 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:21:48.36 SS.reconfigure SSinhale.with maxSpeed: 39 @18:21:48.41 SS.seek 25ml @18:22:34.26 Stroke completed -- waiting for pressure to equalize ... @18:22:38.10 Intake.select :close @18:22:38.27 SSV.dial :exhaust @18:22:39.13 Exhaust.select :open @18:22:39.24 SS.reconfigure SSexhale @18:22:39.30 SS.seek 0ml @18:22:49.74 Exhaust.select :close @18:22:49.86 SSV.dial CBV @18:22:49.89
CBV.dial SSV @18:22:50.75 Allowing 130s for 25.0ml of remaining 800.0ml @18:22:50.76 Intake.select :open @18:22:52.93 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:22:52.95 SS.reconfigure SSinhale.with maxSpeed: 39 @18:22:53.00 SS.seek 25ml @18:23:38.13 Stroke completed -- waiting for pressure to equalize ... @18:23:40.72 Intake.select :close @18:23:40.87 SSV.dial :exhaust @18:23:41.73 Exhaust.select :open @18:23:41.85 SS.reconfigure SSexhale @18:23:41.91 SS.seek 0ml @18:23:51.73 Exhaust.select :close @18:23:51.85 SSV.dial CBV @18:23:51.88
CBV.dial SSV @18:23:52.77 Allowing 130s for 25.0ml of remaining 775.0ml @18:23:52.78 Intake.select :open @18:23:54.96 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:23:54.98 SS.reconfigure SSinhale.with maxSpeed: 39 @18:23:55.03 SS.seek 25ml @18:24:40.51 Stroke completed -- waiting for pressure to equalize ... @18:24:43.74 Intake.select :close @18:24:43.90 SSV.dial :exhaust @18:24:44.75 Exhaust.select :open @18:24:44.86 SS.reconfigure SSexhale @18:24:44.91 SS.seek 0ml @18:24:54.69 Exhaust.select :close @18:24:54.81 SSV.dial CBV @18:24:54.84
CBV.dial SSV @18:24:55.72 Allowing 130s for 25.0ml of remaining 750.0ml @18:24:55.73 Intake.select :open @18:24:57.90 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:24:57.92 SS.reconfigure SSinhale.with maxSpeed: 39 @18:24:57.97 SS.seek 25ml @18:25:43.44 Stroke completed -- waiting for pressure to equalize ... @18:25:46.65 Intake.select :close @18:25:46.81 SSV.dial :exhaust @18:25:47.66 Exhaust.select :open @18:25:47.77 SS.reconfigure SSexhale @18:25:47.82 SS.seek 0ml @18:25:57.35 Exhaust.select :close @18:25:57.47 SSV.dial CBV @18:25:57.50
CBV.dial SSV @18:25:58.38 Allowing 130s for 25.0ml of remaining 725.0ml @18:25:58.39 Intake.select :open @18:26:00.56 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:26:00.58 SS.reconfigure SSinhale.with maxSpeed: 39 @18:26:00.63 SS.seek 25ml @18:26:46.44 Stroke completed -- waiting for pressure to equalize ... @18:26:49.65 Intake.select :close @18:26:49.80 SSV.dial :exhaust @18:26:50.66 Exhaust.select :open @18:26:50.78 SS.reconfigure SSexhale @18:26:50.82 SS.seek 0ml @18:27:00.38 Exhaust.select :close @18:27:00.50 SSV.dial CBV @18:27:00.53
CBV.dial SSV @18:27:01.41 Allowing 130s for 25.0ml of remaining 700.0ml @18:27:01.42 Intake.select :open @18:27:03.59 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:27:03.61 SS.reconfigure SSinhale.with maxSpeed: 39 @18:27:03.66 SS.seek 25ml @18:27:49.97 Stroke completed -- waiting for pressure to equalize ... @18:27:53.22 Intake.select :close @18:27:53.38 SSV.dial :exhaust @18:27:54.22 Exhaust.select :open @18:27:54.34 SS.reconfigure SSexhale @18:27:54.40 SS.seek 0ml @18:28:03.90 Exhaust.select :close @18:28:04.02 SSV.dial CBV @18:28:04.05
CBV.dial SSV @18:28:04.94 Allowing 130s for 25.0ml of remaining 675.0ml @18:28:04.95 Intake.select :open @18:28:07.13 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:28:07.14 SS.reconfigure SSinhale.with maxSpeed: 39 @18:28:07.19 SS.seek 25ml @18:28:53.05 Stroke completed -- waiting for pressure to equalize ... @18:28:56.25 Intake.select :close @18:28:56.40 SSV.dial :exhaust @18:28:57.26 Exhaust.select :open @18:28:57.38 SS.reconfigure SSexhale @18:28:57.43 SS.seek 0ml @18:29:07.08 Exhaust.select :close @18:29:07.20 SSV.dial CBV @18:29:07.23
CBV.dial SSV @18:29:08.09 Allowing 130s for 25.0ml of remaining 650.0ml @18:29:08.10 Intake.select :open @18:29:10.28 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:29:10.30 SS.reconfigure SSinhale.with maxSpeed: 39 @18:29:10.35 SS.seek 25ml @18:29:56.37 Stroke completed -- waiting for pressure to equalize ... @18:30:00.18 Intake.select :close @18:30:00.33 SSV.dial :exhaust @18:30:01.18 Exhaust.select :open @18:30:01.30 SS.reconfigure SSexhale @18:30:01.36 SS.seek 0ml @18:30:10.99 Exhaust.select :close @18:30:11.11 SSV.dial CBV @18:30:11.14
CBV.dial SSV @18:30:12.01 Allowing 130s for 25.0ml of remaining 625.0ml @18:30:12.02 Intake.select :open @18:30:14.19 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:30:14.21 SS.reconfigure SSinhale.with maxSpeed: 39 @18:30:14.26 SS.seek 25ml @18:30:59.96 Stroke completed -- waiting for pressure to equalize ... @18:31:03.17 Intake.select :close @18:31:03.33 SSV.dial :exhaust @18:31:04.19 Exhaust.select :open @18:31:04.30 SS.reconfigure SSexhale @18:31:04.35 SS.seek 0ml @18:31:14.08 Exhaust.select :close @18:31:14.20 SSV.dial CBV @18:31:14.23
CBV.dial SSV @18:31:15.09 Allowing 130s for 25.0ml of remaining 600.0ml @18:31:15.10 Intake.select :open @18:31:17.27 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:31:17.29 SS.reconfigure SSinhale.with maxSpeed: 39 @18:31:17.34 SS.seek 25ml @18:32:02.86 Stroke completed -- waiting for pressure to equalize ... @18:32:06.53 Intake.select :close @18:32:06.69 SSV.dial :exhaust @18:32:07.55 Exhaust.select :open @18:32:07.66 SS.reconfigure SSexhale @18:32:07.71 SS.seek 0ml @18:32:17.43 Exhaust.select :close @18:32:17.55 SSV.dial CBV @18:32:17.58
CBV.dial SSV @18:32:18.45 Allowing 130s for 25.0ml of remaining 575.0ml @18:32:18.46 Intake.select :open @18:32:20.63 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:32:20.65 SS.reconfigure SSinhale.with maxSpeed: 39 @18:32:20.70 SS.seek 25ml @18:33:06.21 Stroke completed -- waiting for pressure to equalize ... @18:33:09.44 Intake.select :close @18:33:09.59 SSV.dial :exhaust @18:33:10.45 Exhaust.select :open @18:33:10.56 SS.reconfigure SSexhale @18:33:10.61 SS.seek 0ml @18:33:20.53 Exhaust.select :close @18:33:20.65 SSV.dial CBV @18:33:20.68
CBV.dial SSV @18:33:21.56 Allowing 130s for 25.0ml of remaining 550.0ml @18:33:21.57 Intake.select :open @18:33:23.74 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:33:23.76 SS.reconfigure SSinhale.with maxSpeed: 39 @18:33:23.81 SS.seek 25ml @18:34:08.83 Stroke completed -- waiting for pressure to equalize ... @18:34:12.12 Intake.select :close @18:34:12.28 SSV.dial :exhaust @18:34:13.14 Exhaust.select :open @18:34:13.25 SS.reconfigure SSexhale @18:34:13.30 SS.seek 0ml @18:34:22.88 Exhaust.select :close @18:34:23.00 SSV.dial CBV @18:34:23.03
CBV.dial SSV @18:34:23.89 Allowing 130s for 25.0ml of remaining 525.0ml @18:34:23.90 Intake.select :open @18:34:26.07 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:34:26.09 SS.reconfigure SSinhale.with maxSpeed: 39 @18:34:26.14 SS.seek 25ml @18:35:11.78 Stroke completed -- waiting for pressure to equalize ... @18:35:14.99 Intake.select :close @18:35:15.14 SSV.dial :exhaust @18:35:15.99 Exhaust.select :open @18:35:16.11 SS.reconfigure SSexhale @18:35:16.17 SS.seek 0ml @18:35:25.89 Exhaust.select :close @18:35:26.01 SSV.dial CBV @18:35:26.04
CBV.dial SSV @18:35:26.90 Allowing 130s for 25.0ml of remaining 500.0ml @18:35:26.91 Intake.select :open @18:35:29.08 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:35:29.10 SS.reconfigure SSinhale.with maxSpeed: 39 @18:35:29.15 SS.seek 25ml @18:36:14.58 Stroke completed -- waiting for pressure to equalize ... @18:36:17.79 Intake.select :close @18:36:18.40 SSV.dial :exhaust @18:36:19.26 Exhaust.select :open @18:36:19.37 SS.reconfigure SSexhale @18:36:19.42 SS.seek 0ml @18:36:28.91 Exhaust.select :close @18:36:29.03 SSV.dial CBV @18:36:29.06
CBV.dial SSV @18:36:29.93 Allowing 130s for 25.0ml of remaining 475.0ml @18:36:29.94 Intake.select :open @18:36:32.13 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:36:32.14 SS.reconfigure SSinhale.with maxSpeed: 39 @18:36:32.20 SS.seek 25ml @18:37:17.89 Stroke completed -- waiting for pressure to equalize ... @18:37:21.75 Intake.select :close @18:37:21.91 SSV.dial :exhaust @18:37:22.76 Exhaust.select :open @18:37:22.87 SS.reconfigure SSexhale @18:37:22.93 SS.seek 0ml @18:37:32.50 Exhaust.select :close @18:37:32.62 SSV.dial CBV @18:37:32.65
CBV.dial SSV @18:37:33.51 Allowing 130s for 25.0ml of remaining 450.0ml @18:37:33.52 Intake.select :open @18:37:35.69 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:37:35.71 SS.reconfigure SSinhale.with maxSpeed: 39 @18:37:35.76 SS.seek 25ml @18:38:21.78 Stroke completed -- waiting for pressure to equalize ... @18:38:24.99 Intake.select :close @18:38:25.15 SSV.dial :exhaust @18:38:26.00 Exhaust.select :open @18:38:26.11 SS.reconfigure SSexhale @18:38:26.16 SS.seek 0ml @18:38:35.77 Exhaust.select :close @18:38:35.89 SSV.dial CBV @18:38:35.92
CBV.dial SSV @18:38:36.78 Allowing 130s for 25.0ml of remaining 425.0ml @18:38:36.79 Intake.select :open @18:38:38.96 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:38:38.98 SS.reconfigure SSinhale.with maxSpeed: 39 @18:38:39.03 SS.seek 25ml @18:39:24.70 Stroke completed -- waiting for pressure to equalize ... @18:39:28.53 Intake.select :close @18:39:28.69 SSV.dial :exhaust @18:39:29.53 Exhaust.select :open @18:39:29.65 SS.reconfigure SSexhale @18:39:29.70 SS.seek 0ml @18:39:39.40 Exhaust.select :close @18:39:39.51 SSV.dial CBV @18:39:39.54
CBV.dial SSV @18:39:40.40 Allowing 130s for 25.0ml of remaining 400.0ml @18:39:40.41 Intake.select :open @18:39:42.58 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:39:42.60 SS.reconfigure SSinhale.with maxSpeed: 39 @18:39:42.65 SS.seek 25ml @18:40:28.06 Stroke completed -- waiting for pressure to equalize ... @18:40:31.27 Intake.select :close @18:40:31.43 SSV.dial :exhaust @18:40:32.65 Exhaust.select :open @18:40:32.84 SS.reconfigure SSexhale @18:40:32.89 SS.seek 0ml @18:40:42.45 Exhaust.select :close @18:40:42.57 SSV.dial CBV @18:40:42.60
CBV.dial SSV @18:40:43.46 Allowing 130s for 25.0ml of remaining 375.0ml @18:40:43.47 Intake.select :open @18:40:45.64 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:40:45.66 SS.reconfigure SSinhale.with maxSpeed: 39 @18:40:45.71 SS.seek 25ml @18:41:31.36 Stroke completed -- waiting for pressure to equalize ... @18:41:34.60 Intake.select :close @18:41:34.76 SSV.dial :exhaust @18:41:35.62 Exhaust.select :open @18:41:35.73 SS.reconfigure SSexhale @18:41:35.78 SS.seek 0ml @18:41:45.30 Exhaust.select :close @18:41:45.42 SSV.dial CBV @18:41:45.45
CBV.dial SSV @18:41:46.32 Allowing 130s for 25.0ml of remaining 350.0ml @18:41:46.33 Intake.select :open @18:41:48.50 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:41:48.52 SS.reconfigure SSinhale.with maxSpeed: 39 @18:41:48.57 SS.seek 25ml @18:42:34.41 Stroke completed -- waiting for pressure to equalize ... @18:42:38.23 Intake.select :close @18:42:38.39 SSV.dial :exhaust @18:42:39.24 Exhaust.select :open @18:42:39.35 SS.reconfigure SSexhale @18:42:39.40 SS.seek 0ml @18:42:49.00 Exhaust.select :close @18:42:49.12 SSV.dial CBV @18:42:49.15
CBV.dial SSV @18:42:50.01 Allowing 130s for 25.0ml of remaining 325.0ml @18:42:50.02 Intake.select :open @18:42:52.19 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:42:52.20 SS.reconfigure SSinhale.with maxSpeed: 39 @18:42:52.25 SS.seek 25ml @18:43:37.87 Stroke completed -- waiting for pressure to equalize ... @18:43:41.08 Intake.select :close @18:43:41.24 SSV.dial :exhaust @18:43:42.08 Exhaust.select :open @18:43:42.20 SS.reconfigure SSexhale @18:43:42.25 SS.seek 0ml @18:43:51.89 Exhaust.select :close @18:43:52.01 SSV.dial CBV @18:43:52.04
CBV.dial SSV @18:43:52.90 Allowing 130s for 25.0ml of remaining 300.0ml @18:43:52.91 Intake.select :open @18:43:55.08 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:43:55.10 SS.reconfigure SSinhale.with maxSpeed: 39 @18:43:55.15 SS.seek 25ml @18:44:40.66 Stroke completed -- waiting for pressure to equalize ... @18:44:43.88 Intake.select :close @18:44:44.03 SSV.dial :exhaust @18:44:44.88 Exhaust.select :open @18:44:44.99 SS.reconfigure SSexhale @18:44:45.04 SS.seek 0ml @18:44:54.59 Exhaust.select :close @18:44:54.71 SSV.dial CBV @18:44:55.19
CBV.dial SSV @18:44:56.07 Allowing 130s for 25.0ml of remaining 275.0ml @18:44:56.08 Intake.select :open @18:44:58.25 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:44:58.27 SS.reconfigure SSinhale.with maxSpeed: 39 @18:44:58.33 SS.seek 25ml @18:45:44.06 Stroke completed -- waiting for pressure to equalize ... @18:45:47.92 Intake.select :close @18:45:48.08 SSV.dial :exhaust @18:45:48.93 Exhaust.select :open @18:45:49.04 SS.reconfigure SSexhale @18:45:49.10 SS.seek 0ml @18:45:58.66 Exhaust.select :close @18:45:58.78 SSV.dial CBV @18:45:58.81
CBV.dial SSV @18:45:59.70 Allowing 130s for 25.0ml of remaining 250.0ml @18:45:59.71 Intake.select :open @18:46:01.88 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:46:01.90 SS.reconfigure SSinhale.with maxSpeed: 39 @18:46:01.95 SS.seek 25ml @18:46:47.64 Stroke completed -- waiting for pressure to equalize ... @18:46:51.54 Intake.select :close @18:46:51.70 SSV.dial :exhaust @18:46:52.56 Exhaust.select :open @18:46:52.67 SS.reconfigure SSexhale @18:46:52.77 SS.seek 0ml @18:47:02.39 Exhaust.select :close @18:47:02.51 SSV.dial CBV @18:47:02.54
CBV.dial SSV @18:47:03.40 Allowing 130s for 25.0ml of remaining 225.0ml @18:47:03.41 Intake.select :open @18:47:05.58 Intake @15.4psia, vacuum from 8.9 to 12.9psi @18:47:05.60 SS.reconfigure SSinhale.with maxSpeed: 39 @18:47:05.65 SS.seek 25ml @18:47:51.23 Stroke completed -- waiting for pressure to equalize ... @18:47:54.45 Intake.select :close @18:47:54.60 SSV.dial :exhaust @18:47:55.46 Exhaust.select :open @18:47:55.57 SS.reconfigure SSexhale @18:47:55.62 SS.seek 0ml @18:48:05.34 Exhaust.select :close @18:48:05.46 SSV.dial CBV @18:48:05.49
CBV.dial SSV @18:48:06.35 Allowing 130s for 25.0ml of remaining 200.0ml @18:48:06.36 Intake.select :open @18:48:08.53 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:48:08.54 SS.reconfigure SSinhale.with maxSpeed: 39 @18:48:08.59 SS.seek 25ml @18:48:53.98 Stroke completed -- waiting for pressure to equalize ... @18:48:57.20 Intake.select :close @18:48:57.36 SSV.dial :exhaust @18:48:58.21 Exhaust.select :open @18:48:58.32 SS.reconfigure SSexhale @18:48:58.37 SS.seek 0ml @18:49:08.31 Exhaust.select :close @18:49:08.43 SSV.dial CBV @18:49:08.46
CBV.dial SSV @18:49:09.34 Allowing 130s for 25.0ml of remaining 175.0ml @18:49:09.35 Intake.select :open @18:49:11.99 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:49:12.00 SS.reconfigure SSinhale.with maxSpeed: 39 @18:49:12.05 SS.seek 25ml @18:49:57.67 Stroke completed -- waiting for pressure to equalize ... @18:50:00.27 Intake.select :close @18:50:00.43 SSV.dial :exhaust @18:50:01.28 Exhaust.select :open @18:50:01.40 SS.reconfigure SSexhale @18:50:01.45 SS.seek 0ml @18:50:11.09 Exhaust.select :close @18:50:11.21 SSV.dial CBV @18:50:11.24
CBV.dial SSV @18:50:12.10 Allowing 130s for 25.0ml of remaining 150.0ml @18:50:12.13 Intake.select :open @18:50:14.34 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:50:14.36 SS.reconfigure SSinhale.with maxSpeed: 39 @18:50:14.42 SS.seek 25ml @18:50:59.78 Stroke completed -- waiting for pressure to equalize ... @18:51:03.00 Intake.select :close @18:51:03.15 SSV.dial :exhaust @18:51:04.01 Exhaust.select :open @18:51:04.12 SS.reconfigure SSexhale @18:51:04.17 SS.seek 0ml @18:51:13.70 Exhaust.select :close @18:51:13.82 SSV.dial CBV @18:51:13.85
CBV.dial SSV @18:51:14.73 Allowing 130s for 25.0ml of remaining 125.0ml @18:51:14.74 Intake.select :open @18:51:16.91 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:51:16.93 SS.reconfigure SSinhale.with maxSpeed: 39 @18:51:16.98 SS.seek 25ml @18:52:02.61 Stroke completed -- waiting for pressure to equalize ... @18:52:06.45 Intake.select :close @18:52:06.61 SSV.dial :exhaust @18:52:07.46 Exhaust.select :open @18:52:07.57 SS.reconfigure SSexhale @18:52:07.63 SS.seek 0ml @18:52:17.22 Exhaust.select :close @18:52:17.34 SSV.dial CBV @18:52:17.37
CBV.dial SSV @18:52:18.24 Allowing 130s for 25.0ml of remaining 100.0ml @18:52:18.25 Intake.select :open @18:52:20.42 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:52:20.44 SS.reconfigure SSinhale.with maxSpeed: 39 @18:52:20.49 SS.seek 25ml @18:53:06.25 Stroke completed -- waiting for pressure to equalize ... @18:53:10.08 Intake.select :close @18:53:10.24 SSV.dial :exhaust @18:53:11.10 Exhaust.select :open @18:53:11.21 SS.reconfigure SSexhale @18:53:11.27 SS.seek 0ml @18:53:20.92 Exhaust.select :close @18:53:21.04 SSV.dial CBV @18:53:21.07
CBV.dial SSV @18:53:21.93 Allowing 130s for 25.0ml of remaining 74.9ml @18:53:21.94 Intake.select :open @18:53:24.11 Intake @15.4psia, vacuum from 8.9 to 12.9psi @18:53:24.13 SS.reconfigure SSinhale.with maxSpeed: 39 @18:53:24.18 SS.seek 25ml @18:54:09.54 Stroke completed -- waiting for pressure to equalize ... @18:54:13.23 Intake.select :close @18:54:13.39 SSV.dial :exhaust @18:54:14.23 Exhaust.select :open @18:54:14.35 SS.reconfigure SSexhale @18:54:14.40 SS.seek 0ml @18:54:24.01 Exhaust.select :close @18:54:24.13 SSV.dial CBV @18:54:24.16
CBV.dial SSV @18:54:25.02 Allowing 130s for 25.0ml of remaining 49.9ml @18:54:25.03 Intake.select :open @18:54:27.21 Intake @15.4psia, vacuum from 8.9 to 12.9psi @18:54:27.22 SS.reconfigure SSinhale.with maxSpeed: 39 @18:54:27.27 SS.seek 25ml @18:55:12.70 Stroke completed -- waiting for pressure to equalize ... @18:55:16.54 Intake.select :close @18:55:16.70 SSV.dial :exhaust @18:55:17.54 Exhaust.select :open @18:55:17.65 SS.reconfigure SSexhale @18:55:17.70 SS.seek 0ml @18:55:27.35 Exhaust.select :close @18:55:27.47 SSV.dial CBV @18:55:27.50
CBV.dial SSV @18:55:28.37 Allowing 130s for 24.9ml of remaining 24.9ml @18:55:28.38 Intake.select :open @18:55:30.56 Intake @15.4psia, vacuum from 8.9 to 12.9psi @18:55:30.57 SS.reconfigure SSinhale.with maxSpeed: 39 @18:55:30.62 SS.seek 24.95ml @18:56:15.98 Stroke completed -- waiting for pressure to equalize ... @18:56:19.19 Intake.select :close @18:56:19.35 SSV.dial :exhaust @18:56:20.21 Exhaust.select :open @18:56:20.32 SS.reconfigure SSexhale @18:56:20.37 SS.seek 0ml @18:56:29.93 Exhaust.select :close @18:56:30.06 Sampled 1500.0ml @18:56:30.08 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @18:56:30.15 CSV.dial :waste @18:56:30.23 CS.seek 0ml @18:56:30.33 SSV.dial CBV,avoiding: :exhaust @18:56:30.36 CSV.dial :air @18:56:30.43
CBV.dial SSV @18:56:31.28 CS.seek 10ml @18:56:32.51
CTV.dial :air @18:56:33.06 Gate.power :sampler,:OFF @18:56:33.36
delay 15 seconds @18:56:48.39 SSV.dial :spit,avoiding: :exhaust @18:56:49.86 CTV.dial CSV,avoiding: :intake @18:56:49.92
SS.seek 0ml @18:56:50.03 SSV.dial CBV,avoiding: :exhaust @18:56:58.09 CBV.dial between SSV and waste2 @18:56:58.53 CTV.dial CSV,avoiding: :air @18:56:58.56
CSV.dial CTV @18:56:59.05 CS.reconfigure CSpressurize @18:56:59.10 CS.seek 0ml @18:57:25.72 CSV.dial :air @18:57:26.22 CS.reconfigure CSfast @18:57:26.28 CS.seek 10ml @18:57:52.72 CSV.dial between air and CTV @18:57:53.09 CS.seek 2.516ml @18:58:13.13 CTV.dial CSV,avoiding: :air @18:58:13.16
CSV.dial CTV @18:58:13.50 CS.reconfigure CSpressurize @18:58:13.55 CS.seek 0ml @18:58:17.19 BubblePoint: Intake @68.9psia, Outlet @13.5psia @18:58:17.20 CBV.dial SSV @18:58:17.55 SS.reconfigure SSconfig @18:58:17.60 SS.seek 10ml @18:58:23.57 delay 15 seconds @18:58:38.70 Evacuated: Intake @38.1psia, Outlet @10.4psia @18:58:38.72 SSV.dial :spit,avoiding: :exhaust @18:58:40.20 SS.reconfigure SSexhale @18:58:40.25 SS.seek 0ml @18:58:45.53 SS.reconfigure SSfast @18:58:45.58 SS.seek 5ml @18:58:48.63 SSV.dial CBV,avoiding: :exhaust @18:58:48.66
CBV.dial SSV @18:58:50.16 CTV.dial :air,avoiding: :intake @18:58:51.66 CSV.dial CTV @18:58:51.71
SS.seek 10ml @18:58:52.70 CSV.dial :air @18:58:53.22 CS.reconfigure CSfast @18:58:53.27 CS.seek 0ml @18:58:54.38
SSV.dial :spit,avoiding: :exhaust @18:58:55.86 SS.reconfigure SSexhale @18:58:55.91 SS.seek 0ml @18:58:57.78 CS.coast @18:59:01.42
SS.reconfigure SSfast @18:59:01.47 SS.seek 5ml @18:59:04.33 SSV.dial CBV,avoiding: :exhaust @18:59:04.36
CBV.dial SSV @18:59:06.86 SSV.dial :spit,avoiding: :exhaust @18:59:08.33 SS.reconfigure SSconfig @18:59:08.38 SS.seek 0ml @18:59:11.53 SS.coast @18:59:11.58 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @18:59:11.59 Intake.select :close @18:59:11.67 CSV.dial :waste @18:59:11.82 SSV.dial CBV,avoiding: :exhaust @18:59:11.85
CBV.dial SSV @18:59:12.55 CS.seek 0ml @18:59:12.62 CSV.dial :air @18:59:13.36
CTV.dial :air @18:59:13.37 delay 15 seconds @18:59:13.47 CS.seek 10ml @18:59:28.40
SSV.dial :spit,avoiding: :exhaust @18:59:29.88 CTV.dial CSV,avoiding: :intake @18:59:29.92
SS.reconfigure SSexhale @18:59:30.03 SS.seek 0ml @18:59:30.54 SSV.dial CBV,avoiding: :exhaust @18:59:40.03 CBV.dial between SSV and waste2 @18:59:40.46 CSV.dial between air and CTV @18:59:40.80 CS.seek 8.277ml @18:59:46.19 CTV.dial CSV,avoiding: :air @18:59:46.22
CSV.dial CTV @18:59:47.02 CS.reconfigure CSpressurize @18:59:47.07 CS.seek 0ml @19:00:08.85 CSV.dial :air @19:00:09.37 CS.reconfigure CSfast @19:00:09.42 CS.seek 10ml @19:00:36.03 CSV.dial between air and CTV @19:00:36.39 CS.seek 3.073ml @19:00:55.19 CTV.dial CSV,avoiding: :air @19:00:55.22
CSV.dial CTV @19:00:55.69 CS.reconfigure CSpressurize @19:00:55.73 CS.seek 0ml @19:01:03.25 BubblePoint: Intake @68.3psia, Outlet @13.1psia @19:01:03.26 CBV.dial SSV @19:01:03.61 SS.reconfigure SSconfig @19:01:03.66 SS.seek 10ml @19:01:09.27 delay 15 seconds @19:01:24.41 Evacuated: Intake @40.6psia, Outlet @14.0psia @19:01:24.42 SSV.dial :spit,avoiding: :exhaust @19:01:25.93 SS.reconfigure SSexhale @19:01:25.99 SS.seek 0ml @19:01:31.29 SS.reconfigure SSfast @19:01:31.34 SS.seek 5ml @19:01:34.08 SSV.dial CBV,avoiding: :exhaust @19:01:34.11
CBV.dial SSV @19:01:35.60 CTV.dial :air,avoiding: :intake @19:01:37.10 CSV.dial CTV @19:01:37.15
SS.seek 10ml @19:01:38.14 CSV.dial :air @19:01:38.65 CS.reconfigure CSfast @19:01:38.70 CS.seek 0ml @19:01:39.68
SSV.dial :spit,avoiding: :exhaust @19:01:40.75 CS.coast @19:01:41.16
SS.reconfigure SSexhale @19:01:41.21 SS.seek 0ml @19:01:46.78 SS.reconfigure SSfast @19:01:46.84 SS.seek 5ml @19:01:49.49 SSV.dial CBV,avoiding: :exhaust @19:01:49.52
CBV.dial SSV @19:01:52.03 SSV.dial :spit,avoiding: :exhaust @19:01:53.52 SS.reconfigure SSconfig @19:01:53.57 SS.seek 0ml @19:01:56.88 SS.coast @19:01:56.93 Lysing: Adding 1.5ml lysis @19:01:56.94 CBV.dial between CSV and waste1,avoiding: :DBV @19:01:58.61 CTV.dial CSV @19:02:00.09 CSV.dial CSR @19:02:01.11 Pulling 1ml of CSR.lysis @19:02:01.12 CSR.select :lysis @19:02:01.24 CS.reconfigure CSconfig @19:02:01.29 CS.seek 1ml @19:02:04.82 delay 5 seconds @19:02:09.84 CSR.select :air @19:02:10.02 CS.seek 6ml @19:02:24.01 delay 2 seconds @19:02:26.02 CSV.dial :waste1 @19:02:27.49 CS.reconfigure CSfast @19:02:27.54 CS.seek :empty @19:02:43.68 CSV.dial CSR @19:02:45.21 Pulling 1.5ml of CSR.lysis @19:02:45.22 CSR.select :lysis @19:02:45.34 CS.reconfigure CSconfig @19:02:45.39 CS.seek 1.5ml @19:02:50.32 delay 5 seconds @19:02:55.34 CSR.select :air @19:02:55.53 CS.seek 4.501ml @19:03:04.12 delay 2 seconds @19:03:06.14 CSV.dial CTV @19:03:07.10 CS.seek 1.275ml @19:03:16.41 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @19:03:16.43 CH.seek 85C,8:00 @19:06:28.08 CH.hold 5:00,85C @19:11:28.26 Collection Puck Heater at 85.2C after lysing @19:11:28.28 CS.seek 0ml @19:11:32.51 CTV.dial between spare and air,avoiding: :intake @19:11:33.68 CSV.dial :air @19:11:34.19 CS.seek 5ml @19:11:48.00 CSV.dial CTV @19:11:48.51 delay 2 seconds @19:11:50.53 CSV.dial :air @19:11:51.04 delay 2 seconds @19:11:53.06 CSV.dial CTV @19:11:53.59 delay 2 seconds @19:11:55.61 CSV.dial :air @19:11:56.12 delay 2 seconds @19:11:58.14 CS.seek 0ml @19:12:11.89 Recovering Lysate into Collection syringe @19:12:11.90 CSV.dial CBV @19:12:13.15 CBV.dial CSV @19:12:13.56 CS.seek 5ml @19:12:27.32 delay 5 seconds @19:12:32.34 CTV.dial :air @19:12:32.94 CS.seek 7.001ml @19:12:39.04 delay 5 seconds @19:12:44.06 CSV.dial :air2 @19:12:44.59 CS.seek 10ml @19:12:53.23 CSV.dial between air and CTV,avoiding: CBV @19:12:55.03 Lysing Complete @19:12:55.12 Storing sh1 puck in Collection Clamp to tube 1 @19:12:55.31 SC.reconfigure SCtight @19:12:55.36 SC.seek 1 @19:12:57.51 SC.reconfigure SCloose @19:12:57.74 Elbow.reconfigure TightElbow @19:12:57.79 Elbow.seek 1 @19:12:59.70 Forearm.reconfigure TightForearm @19:12:59.75 Forearm.seek 1 + 700 counts @19:13:00.73 SE.reconfigure SEpick @19:13:00.80 SE.seek beamBreak @19:13:03.78 SE.configure SEconfig @19:13:03.84 SE.seek :place @19:13:04.44 Forearm.reconfigure LooseForearm @19:13:04.49 Forearm.seek :retract @19:13:05.42 Elbow.seek CC @19:13:07.12 Elbow.reconfigure LooseElbow @19:13:07.23 Forearm.seek CC @19:13:08.25 Hand.close @19:13:08.98 CC.seek :open @19:13:13.51 Forearm.seek :retract @19:13:14.51 Elbow.reconfigure TightElbow @19:13:14.56 Elbow.seek 1 @19:13:16.55 Elbow.reconfigure LimpElbow @19:13:16.61 Forearm.seek 1 @19:13:17.67 Elbow.hold @19:13:17.74 Elbow.reconfigure TightElbow @19:13:17.79 Hand.open @19:13:18.62 Forearm.force -20 @19:13:18.66 Forearm.seek 1 + 349 counts @19:13:18.98 Forearm.reconfigure TightForearm @19:13:19.03 Forearm.seek 1 + 700 counts @19:13:19.36 SE.seek :down @19:13:20.51
Forearm.reconfigure LooseForearm @19:13:20.56 Forearm.seek :retract @19:13:22.15 SE.coast @19:13:22.19
SC.coast @19:13:22.23 Tranferring lysate to PRV @19:13:22.24 CSV.dial :air @19:13:22.57 CS.seek 10ml @19:13:22.64 CSV.dial between air and CTV @19:13:23.00 AIV.dial :bypass @19:13:23.09 Beginning samplePassC @19:13:23.10 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:13:23.18 Hand.open @19:13:23.63 Elbow.seek FlushPuck::Garage @19:13:26.10 Elbow.reconfigure LooseElbow @19:13:26.21 Forearm.seek :garage @19:13:27.26 Hand.close @19:13:28.12 Forearm.seek :retract @19:13:29.18 Elbow.reconfigure TightElbow @19:13:29.23 Elbow.seek CC @19:13:32.13 Elbow.reconfigure LooseElbow @19:13:32.24 Forearm.reconfigure TightForearm @19:13:32.29 Forearm.seek CC @19:13:33.63 CC.seek :closed @19:13:37.92 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.40 @19:13:37.99 Hand.open @19:13:38.80 Forearm.reconfigure LooseForearm @19:13:38.85 Forearm.seek :retract @19:13:39.84 AWV.select :air @19:13:39.85 AIV.dial :bypass @19:13:39.87 PSV.dial PSR @19:13:41.40 Pulling 1.5ml of PSR.flush @19:13:41.41 PSR.select :flush @19:13:41.54 PS.seek 1.5ml @19:13:57.70 delay 5 seconds @19:14:02.72 PSV.dial :waste1 @19:14:03.36 PS.seek 0ml @19:14:19.62 PSV.dial PSR @19:14:20.12 PSR.select :air @19:14:20.25 PS.seek 2.5ml @19:14:46.85 PRV.dial CBV @19:14:46.88
CBV.dial PRV @19:14:47.78 PSV.dial :mixloop @19:14:48.91 CTV.dial :air,avoiding: :intake @19:14:48.92 PTV.dial :PRVmixing @19:14:49.02 PS.seek 0ml @19:15:15.53 PSV.dial :air @19:15:16.05 PS.seek 2.5ml @19:15:42.69 PSV.dial :mixloop @19:15:43.22 PS.seek 0ml @19:16:09.84 PSV.dial :air @19:16:10.36 PS.seek 2.5ml @19:16:36.97 PSV.dial :mixloop @19:16:37.50 PS.seek 0ml @19:17:04.13 Garaging FlushPuck in Collection Clamp @19:17:04.21 Elbow.reconfigure TightElbow @19:17:04.26 Elbow.seek CC @19:17:04.51 Elbow.reconfigure LooseElbow @19:17:04.62 Forearm.seek CC @19:17:05.58 Hand.close @19:17:06.36 CC.seek :open @19:17:10.77 Forearm.seek :retract @19:17:11.80 Elbow.reconfigure TightElbow @19:17:11.84 Elbow.seek :garage @19:17:15.42 Elbow.reconfigure LooseElbow @19:17:15.54 Forearm.reconfigure TightForearm @19:17:15.59 Forearm.seek :garage @19:17:16.74 Hand.open @19:17:17.55 Forearm.reconfigure LooseForearm @19:17:17.60 Forearm.seek :retract @19:17:18.67 Connecting PS to CS to transfer sample @19:17:18.68 CBV.dial AIV @19:17:18.69 AIV.dial :bypass @19:17:18.70 PSV.dial :mixloop @19:17:18.72 PTV.dial :PRVmixing @19:17:18.73 PRV.dial AIV @19:17:18.74 CTV.dial :air,avoiding: :intake @19:17:18.76 Loading lyfil puck from tube 2 into Collection Clamp @19:17:19.02 SC.reconfigure SCtight @19:17:19.07 SC.seek 2 @19:17:21.27 SC.reconfigure SCloose @19:17:21.50 Elbow.reconfigure TightElbow @19:17:21.54 Elbow.seek 2 @19:17:23.70 Forearm.reconfigure TightForearm @19:17:23.75 Forearm.seek 2 + 700 counts @19:17:24.99 SE.reconfigure SEpick @19:17:25.06 SE.seek beamBreak @19:17:28.18 SE.configure SEconfig @19:17:28.36 Elbow.seek 2 @19:17:28.58 Forearm.seek 2 + 300 counts @19:17:29.09 Forearm.reconfigure LooseForearm @19:17:29.14 Hand.close @19:17:29.88 SE.seek :down @19:17:30.10
Forearm.seek :retract @19:17:31.06 Elbow.seek CC @19:17:32.76 Elbow.reconfigure LooseElbow @19:17:32.84 SE.coast @19:17:32.96
Forearm.reconfigure TightForearm @19:17:33.01 Forearm.seek CC @19:17:34.35 CC.seek :closed @19:17:38.61 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.47 @19:17:38.63 Hand.open @19:17:39.43 Forearm.reconfigure LooseForearm @19:17:39.48 Forearm.seek :retract @19:17:40.49 SC.coast @19:17:40.52 CTV.dial CSV,avoiding: :intake @19:17:42.04 CSV.dial CTV @19:17:42.38 CS.seek 5ml @19:17:56.17 delay 1:20 @19:19:16.21 CS.seek 2.5ml @19:19:23.58 delay 40 seconds @19:20:03.61 CS.seek 0ml @19:20:10.95 delay 1:00 @19:21:10.97 PSV.dial :air @19:21:11.49 PS.seek 2.5ml @19:21:37.91 PSV.dial :mixloop @19:21:38.42 delay 3 seconds @19:21:41.44 PSV.dial :air @19:21:41.96 delay 2 seconds @19:21:43.97 PSV.dial :mixloop @19:21:44.48 delay 3 seconds @19:21:47.97 PSV.dial :waste1 @19:21:49.49 PS.seek :empty @19:22:16.16 CBV.dial :waste1 @19:22:16.69 delay 5 seconds @19:22:21.71 CBV.dial AIV @19:22:22.24 delay 10 seconds @19:22:32.26 CBV.dial :waste1 @19:22:32.80 delay 10 seconds @19:22:42.82 CBV.dial AIV @19:22:43.36 delay 10 seconds @19:22:53.39 CTV.dial CSV,avoiding: :intake @19:22:53.40 CBV.dial between AIV and waste1 @19:22:53.77 CSV.dial :air @19:22:54.28 CS.seek 10ml @19:23:20.88 CSV.dial CTV @19:23:21.40 CS.seek 0ml @19:23:48.26 CBV.dial :waste1 @19:23:48.61 delay 10 seconds @19:23:58.64 CTV.dial :air,avoiding: :intake @19:24:00.20 Storing lyfil puck in Collection Clamp to tube 1 @19:24:00.39 SC.reconfigure SCtight @19:24:00.45 SC.seek 1 @19:24:02.60 SC.reconfigure SCloose @19:24:02.82 Elbow.reconfigure TightElbow @19:24:02.87 Elbow.seek 1 @19:24:04.76 Forearm.reconfigure TightForearm @19:24:04.82 Forearm.seek 1 + 700 counts @19:24:05.87 SE.reconfigure SEpick @19:24:05.94 SE.seek beamBreak @19:24:09.01 SE.configure SEconfig @19:24:09.19 Elbow.reconfigure LimpElbow @19:24:09.25 Forearm.reconfigure LooseForearm @19:24:09.29 Forearm.seek 1 @19:24:09.62 Elbow.hold @19:24:09.70 Elbow.reconfigure TightElbow @19:24:09.81 SE.seek 0.06 Pucks below Top Plate @19:24:10.28 Forearm.force -20 @19:24:10.32 Forearm.seek 1 + 347 counts @19:24:10.65 Forearm.reconfigure TightForearm @19:24:10.71 Forearm.seek 1 + 700 counts @19:24:11.06 SE.seek 1.06 Pucks below Top Plate @19:24:11.67 Forearm.reconfigure LooseForearm @19:24:11.72 Forearm.seek :retract @19:24:12.66 Elbow.seek CC @19:24:14.38 Elbow.reconfigure LooseElbow @19:24:14.49 Forearm.seek CC @19:24:15.47 Hand.close @19:24:16.24 CC.seek :open @19:24:20.78 Forearm.seek :retract @19:24:21.79 Elbow.reconfigure TightElbow @19:24:21.84 Elbow.seek 1 @19:24:23.75 Elbow.reconfigure LimpElbow @19:24:23.80 Forearm.seek 1 @19:24:24.84 Elbow.hold @19:24:24.91 Elbow.reconfigure TightElbow @19:24:24.96 Hand.open @19:24:26.38 Forearm.force -20 @19:24:26.42 Forearm.seek 1 + 348 counts @19:24:26.76 Forearm.reconfigure TightForearm @19:24:26.82 Forearm.seek 1 + 700 counts @19:24:27.29 SE.seek :down @19:24:28.44
Forearm.reconfigure LooseForearm @19:24:28.49 Forearm.seek :retract @19:24:29.99 SE.coast @19:24:30.03
SC.coast @19:24:30.07 PSV.dial :air @19:24:31.29 PS.seek 0.499ml @19:24:37.26 delay 3 seconds @19:24:40.27 PSV.dial :mixloop @19:24:40.78 PRV.dial :air @19:24:41.29 delay 3 seconds @19:24:44.30 PRV.dial CBV @19:24:44.82 CBV.dial AIV @19:24:45.34 PRV.dial between CBV and air @19:24:45.70 PRV.dial :air @19:24:46.11 PS.reconfigure PSslow @19:24:46.16 PS.seek 0.599ml @19:24:48.69 PRV.dial :waste2 @19:24:49.54 PS.reconfigure PSconfig @19:24:49.59 PS.seek :empty @19:24:56.51 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:24:56.58 Hand.open @19:24:57.00 Elbow.seek FlushPuck::Garage @19:24:58.90 Elbow.reconfigure LooseElbow @19:24:59.01 Forearm.seek :garage @19:25:00.05 Hand.close @19:25:00.87 Forearm.seek :retract @19:25:01.91 Elbow.reconfigure TightElbow @19:25:01.96 Elbow.seek CC @19:25:04.85 Elbow.reconfigure LooseElbow @19:25:04.96 Forearm.reconfigure TightForearm @19:25:05.00 Forearm.seek CC @19:25:06.30 CC.seek :closed @19:25:10.58 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.31 @19:25:10.65 Hand.open @19:25:11.44 Forearm.reconfigure LooseForearm @19:25:11.48 Forearm.seek :retract @19:25:12.54 Clearing PML for modified lysate @19:25:12.55 CTV.dial :air,avoiding: :intake @19:25:12.57 PRV.dial :waste2 @19:25:12.58 PTV.dial :PRV,avoiding: :puck @19:25:13.42 delay 2 seconds @19:25:15.44 PTV.dial :PRVmixing,avoiding: :puck @19:25:16.28 delay 2 seconds @19:25:18.30 PSV.dial :PML @19:25:18.31 delay 2 seconds @19:25:20.34 PRV.dial :air @19:25:21.68 delay 2 seconds @19:25:23.79 PS.seek 1.499ml @19:25:40.01 delay 5 seconds @19:25:45.03 PS.seek 2.5ml @19:25:56.20 PSV.dial :air @19:25:56.72 PS.seek 0ml @19:26:23.39 mixing lysate with speDil @19:26:23.40 PSV.dial :PML @19:26:24.03 CBV.dial :PRV @19:26:24.04 PRV.dial :air @19:26:24.06 delay 2 seconds @19:26:26.08 PRV.dial :speDil @19:26:26.60 delay 2 seconds @19:26:28.70 PS.reconfigure PSslow1 @19:26:28.75 PS.seek 0.225ml @19:26:52.82 delay 5 seconds @19:26:57.85 PRV.dial CBV @19:26:58.71 delay 2 seconds @19:27:00.80 PS.seek 0.475ml @19:27:26.54 delay 5 seconds @19:27:31.57 PRV.dial :air @19:27:32.09 delay 2 seconds @19:27:34.13 PS.seek 0.965ml @19:28:24.23 delay 10 seconds @19:28:34.26 PRV.dial CBV @19:28:34.75 CTV.dial :air,avoiding: :intake @19:28:34.77 PSV.dial :air @19:28:35.25 PS.reconfigure PSconfig @19:28:35.30 PS.seek 2.5ml @19:28:51.82 delay 3 seconds @19:28:54.84 PSV.dial PTV @19:28:55.68 PTV.dial :PRV,avoiding: :puck @19:28:56.54 PS.seek 0ml @19:29:23.10 PSV.dial :air @19:29:23.96 PS.seek 2.5ml @19:29:50.57 delay 3 seconds @19:29:53.59 PSV.dial PTV @19:29:54.43 PTV.dial :PRV,avoiding: :puck @19:29:54.45 PS.seek 0ml @19:30:21.11 Pushing modified lysate into AIV Injection Loop @19:30:21.12 CBV.dial AIV @19:30:21.13 AIV.dial :bypass @19:30:21.15 PRV.dial CBV @19:30:21.16 PSV.dial :air @19:30:21.99 PS.seek 2.5ml @19:30:48.56 delay 3 seconds @19:30:51.59 PSV.dial :PML @19:30:52.07 PTV.dial :PRVmixing,avoiding: :puck @19:30:52.97 PS.reconfigure PSslow1 @19:30:53.02 PS.seek 1.74ml @19:32:10.72 delay 5 seconds @19:32:15.75 AIV.dial :sample @19:32:16.38 AWV.select :waste @19:32:16.55 PS.reconfigure PSslow2 @19:32:16.60 PS.seek 1.22ml @19:34:04.76 delay 15 seconds @19:34:19.79 AIV.dial :bypass @19:34:20.41 PS.reconfigure PSconfig @19:34:20.46 PS.seek 0ml @19:34:33.72 PSV.dial :air @19:34:34.35 PS.seek 2.5ml @19:35:00.75 PSV.dial :PML @19:35:01.23 PS.seek 0ml @19:35:27.57 AIV.dial :coilToMFB @19:35:27.59 AWV.select :air @19:35:27.76 Extracting Nucleic Acid from AIV @19:35:27.78
Beginning samplePassD @19:35:27.86 SPE.hold 2:46:40,55C @19:35:27.90 ASV.dial :SPEdelivery @19:35:27.99
AWV.select :air @19:35:28.00 AIV.dial :bypass @19:35:28.02 PSV.dial PSR @19:35:28.35 delay 20 seconds @19:35:29.29
Pulling 1.5ml of PSR.killMFB @19:35:29.30 PSR.select :killMFB @19:35:29.43 PS.seek 1.5ml @19:35:46.39 delay 5 seconds @19:35:48.38 primeLysateINT @19:35:48.39 AWV.select :air @19:35:48.82 AIV.dial :coilToMFB @19:35:48.91 ATV.dial AIV @19:35:50.79 AS.reconfigure ASslow1 @19:35:50.84 AS.seek 0.299ml @19:35:51.42
PSV.dial :waste1 @19:35:51.96 PS.seek 0ml @19:36:08.94 PSV.dial PSR @19:36:09.48 PSR.select :air @19:36:09.61 PS.seek 2.5ml @19:36:37.29 PRV.dial CBV @19:36:37.32
CBV.dial PRV @19:36:37.33 PSV.dial :mixloop @19:36:38.53 CTV.dial :air,avoiding: :intake @19:36:38.54 PTV.dial :PRVmixing @19:36:38.56 PS.seek 0ml @19:36:51.57 delay 10 seconds @19:37:01.59 ATV.dial :air2 @19:37:02.48 AS.seek 0.329ml @19:37:06.38
PSV.dial PSR @19:37:07.67 Pulling 1.5ml of PSR.flush @19:37:07.68 PSR.select :flush @19:37:07.81 PS.seek 1.5ml @19:37:08.88 ATV.dial :waste2 @19:37:09.47 AS.reconfigure ASnorm @19:37:09.53 AS.seek :empty @19:37:24.73
delay 5 seconds @19:37:26.54 Lysate primed! @19:37:26.55 AFV.select :holdingCoil @19:37:26.56 ATV.dial :air2 @19:37:27.22 AS.reconfigure ASslow1 @19:37:27.27 AS.seek 0.069ml @19:37:29.76
PSV.dial :waste1 @19:37:30.41 PS.seek 0ml @19:37:41.83 delay 3 seconds @19:37:44.85 ATV.dial :AIV @19:37:45.75 AS.seek 0.469ml @19:37:47.38
PSV.dial PSR @19:37:48.03 PSR.select :air @19:37:48.16 PS.seek 2.5ml @19:38:15.79 PRV.dial CBV @19:38:15.82
CBV.dial PRV @19:38:15.83 PSV.dial :mixloop @19:38:17.03 CTV.dial :air,avoiding: :intake @19:38:17.04 PTV.dial :PRVmixing @19:38:17.06 PS.seek 0ml @19:38:44.45 PSV.dial PSR @19:38:45.71 Pulling 1.5ml of PSR.flush PSR.select :flush @19:38:45.84 PS.seek 1.5ml @19:39:02.51 delay 5 seconds @19:39:06.49 delay 5 seconds @19:39:07.54
PSV.dial :waste1 @19:39:08.07 PS.seek 0ml @19:39:11.56 Solid Phase Extraction Heater at 50.7C @19:39:11.57 ATV.dial ASV @19:39:11.99 ASV.dial :SPEdelivery @19:39:12.08 AS.seek 0.041ml @19:39:24.70
PSV.dial PSR @19:39:25.34 PSR.select :air @19:39:25.47 PS.seek 2.5ml @19:39:52.67 PRV.dial CBV @19:39:52.69
CBV.dial PRV @19:39:52.72 PSV.dial :mixloop @19:39:53.90 CTV.dial :air,avoiding: :intake @19:39:53.91 PTV.dial :PRVmixing @19:39:53.93 PS.seek 0ml @19:40:20.75 PSV.dial PSR @19:40:21.98 Pulling 1.5ml of PSR.flush @19:40:21.99 PSR.select :flush @19:40:22.12 PS.seek 1.5ml @19:40:38.46 delay 10 seconds @19:40:38.58
delay 5 seconds @19:40:43.61 PSV.dial :waste1 @19:40:44.15 PS.seek 0ml @19:40:48.48 rinseLysateLineINT @19:40:48.49 AFV.select :carrierSolution @19:40:48.59 delay 2 seconds @19:40:50.62 AS.reconfigure ASconfig @19:40:50.67 AS.seek 1ml @19:41:00.42
PSV.dial PSR @19:41:01.06 PSR.select :air @19:41:01.19 PS.seek 2.5ml @19:41:28.14 PRV.dial CBV @19:41:28.17
CBV.dial PRV @19:41:28.18 PSV.dial :mixloop @19:41:29.37 CTV.dial :air,avoiding: :intake @19:41:29.38 PTV.dial :PRVmixing @19:41:29.40 PS.reconfigure PSslow @19:41:29.45 PS.seek 1.2ml @19:41:46.58 delay 1 second @19:41:47.60 AFV.select :holdingCoil @19:41:47.71 delay 1 second @19:41:48.74 ATV.dial AIV @19:41:49.29 AIV.dial :coilToMFB @19:41:49.30 AWV.select :waste @19:41:49.42 AS.reconfigure ASslow0 @19:41:49.47 AS.seek 0ml @19:41:56.23
PSV.dial :waste1 @19:41:57.73 PS.reconfigure PSconfig @19:41:57.79 PS.seek 0ml @19:42:11.05 PSV.dial :air @19:42:12.35 PS.seek 2.5ml @19:42:39.07 PSV.dial :mixloop @19:42:39.72 PS.reconfigure PSslow @19:42:39.78 PS.seek 0ml @19:43:30.37 ATV.dial :air2 @19:43:30.99
PSV.dial :air @19:43:31.13 AS.reconfigure ASslow1 @19:43:31.18 AS.seek 0.05ml @19:43:31.48
PS.reconfigure PSconfig @19:43:31.54 PS.seek 2.5ml @19:43:41.59 ATV.dial :AIV @19:43:42.43 AS.seek 0.01ml @19:43:50.74 ATV.dial :waste @19:43:52.40 AS.seek :empty @19:43:54.83 ethanol wash @19:43:54.84 ATV.dial :air2 @19:43:56.13 AS.reconfigure ASslow2 @19:43:56.18 AS.seek 0.009ml @19:43:58.08
PSV.dial :mixloop @19:43:58.58 PS.reconfigure PSslow @19:43:58.63 PS.seek 0ml @19:44:06.55 delay 3 seconds @19:44:09.58 Pulling 0.4ml of Ethanol Wash @19:44:09.59 ATV.dial :ethanol @19:44:10.43 AS.reconfigure ASslow1 @19:44:10.48 AS.seek 0.409ml @19:44:49.89
Cleaning Collection Syringe @19:44:49.90 CSV.dial CSR @19:44:50.57 Pulling 4ml of CSR.flush @19:44:50.58 CSR.select :flush @19:44:50.71 CS.seek 4ml @19:45:01.87 delay 5 seconds @19:45:06.89 CSR.select :air @19:45:07.03 CS.seek 10ml @19:45:23.53 CSV.dial CTV @19:45:23.56
CTV.dial CSV @19:45:25.11 CBV.dial :waste1 @19:45:25.65 CS.seek 0ml @19:45:31.19 delay 3 seconds @19:45:34.21 ATV.dial :air1 @19:45:34.61 delay 3 seconds @19:45:37.70 AS.seek 0.434ml @19:45:43.01 delay 3 seconds @19:45:46.03 ATV.dial :ethanol @19:45:46.67 AS.seek 0.419ml @19:45:49.96 delay 3 seconds @19:45:52.15
CSV.dial CSR @19:45:52.71 Pulling 4ml of CSR.flush @19:45:52.72 CSR.select :flush @19:45:52.85 CS.seek 4ml @19:45:52.97 ATV.dial ASV @19:45:54.34 AS.seek 0.092ml @19:46:04.37
delay 5 seconds @19:46:09.39 CSR.select :air @19:46:09.53 CS.seek 10ml @19:46:25.90 CSV.dial CTV @19:46:25.92
CTV.dial CSV @19:46:26.97 CBV.dial :waste1 @19:46:26.99 CS.seek 0ml @19:46:53.60 Garaging FlushPuck in Collection Clamp @19:46:53.68 Elbow.reconfigure TightElbow @19:46:53.73 Elbow.seek CC @19:46:53.99 Elbow.reconfigure LooseElbow @19:46:54.10 Forearm.seek CC @19:46:55.10 Hand.close @19:46:55.89 CC.seek :open @19:47:00.28 Forearm.seek :retract @19:47:00.38 delay 3 seconds @19:47:01.28
Elbow.reconfigure TightElbow @19:47:01.33 Elbow.seek :garage @19:47:03.39 ATV.dial :waste2 @19:47:04.16 AS.reconfigure ASconfig @19:47:04.21 AS.seek :empty @19:47:04.48
Elbow.reconfigure LooseElbow @19:47:04.58 Forearm.reconfigure TightForearm @19:47:04.63 Forearm.seek :garage @19:47:05.81 Hand.open @19:47:06.59 Forearm.reconfigure LooseForearm @19:47:06.64 Forearm.seek :retract @19:47:07.72 Skipping Whole Cell Archive @19:47:07.73 Starting finalPurge @19:47:07.75 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:47:07.82 Hand.open @19:47:08.09 Elbow.reconfigure TightElbow @19:47:08.14 Elbow.seek FlushPuck::Garage @19:47:08.29 Elbow.reconfigure LooseElbow @19:47:08.40 Forearm.seek :garage @19:47:09.44 Hand.close @19:47:09.85 ATV.dial :air2 @19:47:10.31 AS.reconfigure ASslow1 @19:47:10.34
Forearm.seek :retract @19:47:10.45 AS.seek 1ml @19:47:11.45
Elbow.reconfigure TightElbow @19:47:11.50 Elbow.seek CC @19:47:14.31 Elbow.reconfigure LooseElbow @19:47:14.42 Forearm.reconfigure TightForearm @19:47:14.47 Forearm.seek CC @19:47:15.70 CC.seek :closed @19:47:19.97 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.38 @19:47:20.04 Hand.open @19:47:20.84 Forearm.reconfigure LooseForearm @19:47:20.89 Forearm.seek :retract @19:47:21.89 Flushing the Collection Valve::Manifold @19:47:22.03 CSV.dial CSR @19:47:22.61 Pulling 5ml of CSR.flush @19:47:22.62 CSR.select :flush @19:47:22.74 CS.seek 5ml @19:47:36.47 delay 5 seconds @19:47:41.49 CSR.select :air @19:47:42.21 CS.reconfigure CSfast @19:47:42.26 CS.seek 7.5ml @19:47:49.61 CSV.dial :waste @19:47:51.08 CS.reconfigure CSconfig @19:47:51.13 CS.seek 0ml @19:48:11.31 Flushing lower manifold's waste1 port @19:48:11.45 CSV.dial :flush @19:48:11.95 CS.reconfigure CSfast @19:48:12.00 CS.seek 5ml @19:48:25.78 delay 5 seconds @19:48:30.80 CSV.dial :air @19:48:31.37 CS.seek 7ml @19:48:37.47 CSV.dial CTV @19:48:37.49
CTV.dial CSV @19:48:38.00 CBV.dial :waste1 @19:48:38.02 CS.seek 0ml @19:48:56.78 Flushing lower manifold's waste2b port @19:48:56.92 CSV.dial :flush @19:48:57.77 CS.seek 5ml @19:49:11.44 delay 5 seconds @19:49:16.46 CSV.dial :air @19:49:17.06 CS.seek 7ml @19:49:23.09 CSV.dial CTV @19:49:23.12
CTV.dial CSV @19:49:23.62 CBV.dial :waste2b @19:49:24.46 CS.seek 0ml @19:49:43.28 Flushing lower manifold's waste1 port @19:49:43.29 CSV.dial CSR @19:49:43.97 Pulling 4ml of CSR.mfbkill @19:49:43.98 CSR.select :mfbkill @19:49:44.11 CS.reconfigure CSconfig @19:49:44.16 CS.seek 4ml @19:49:55.44 delay 5 seconds @19:50:00.46 CSR.select :air @19:50:00.59 CS.reconfigure CSfast @19:50:00.64 CS.seek 10ml @19:50:16.87 CSV.dial CTV @19:50:16.89
CTV.dial CSV @19:50:17.41 CBV.dial :waste1 @19:50:18.27 CS.seek 0ml @19:50:32.08 delay 5 seconds @19:50:37.10 ATV.dial ASV @19:50:38.21 ASV.dial :SPEdelivery @19:50:38.23 AS.seek 0.02ml @19:50:44.50
Flushing lower manifold's waste2b port @19:50:44.51 CSV.dial CSR @19:50:45.09 Pulling 4ml of CSR.mfbkill @19:50:45.10 CSR.select :mfbkill @19:50:45.23 CS.reconfigure CSconfig @19:50:45.27 CS.seek 4ml @19:50:56.53 delay 5 seconds @19:51:01.55 CSR.select :air @19:51:01.71 CS.reconfigure CSfast @19:51:01.76 CS.seek 10ml @19:51:17.97 CSV.dial CTV @19:51:18.00
CTV.dial CSV @19:51:18.52 CBV.dial :waste2b @19:51:19.39 CS.seek 0ml @19:51:45.69 Killing upper manifold @19:51:45.70 CSV.dial CSR @19:51:46.28 Pulling 4ml of CSR.mfbkill @19:51:46.29 CSR.select :mfbkill @19:51:46.42 CS.reconfigure CSconfig @19:51:46.47 CS.seek 4ml @19:51:57.63 delay 5 seconds @19:52:02.65 CSR.select :air @19:52:02.78 CS.reconfigure CSfast @19:52:02.83 CS.seek 10ml @19:52:19.13 CSV.dial CBV @19:52:19.15
CBV.dial CSV @19:52:20.38 CTV.dial :air @19:52:21.89 CS.seek 0ml @19:52:48.25 delay 7 seconds @19:52:55.29 CS.seek 3ml @19:53:03.84 CSV.dial :waste @19:53:04.36 CS.seek 0ml @19:53:12.85 Flushing upper manifold @19:53:13.50 CSV.dial :flush @19:53:14.01 CS.seek 5ml @19:53:28.00 delay 5 seconds @19:53:33.03 CSV.dial :air @19:53:33.61 CS.seek 7ml @19:53:39.66 CSV.dial CBV @19:53:39.69
CBV.dial CSV @19:53:40.86 CTV.dial :air @19:53:40.88 CS.seek 0ml @19:53:55.57 delay 5 seconds @19:53:59.53
delay 7 seconds @19:54:00.59 ATV.dial :air2 @19:54:01.65 AS.seek 0.52ml @19:54:06.55
CS.seek 3ml @19:54:15.12 CSV.dial :waste @19:54:15.65 CS.seek 0ml @19:54:24.31 CSV.dial :flush @19:54:24.82 CS.seek 3ml @19:54:33.50 delay 5 seconds @19:54:38.53 CSV.dial :air @19:54:39.10 CS.seek 5ml @19:54:45.09 CSV.dial :air @19:54:45.12 CS.seek 0ml @19:54:58.69 delay 7 seconds @19:55:05.73 CS.seek 3ml @19:55:14.44 CSV.dial :waste @19:55:15.29 CS.seek 0ml @19:55:23.85 Flushing lower manifold's waste1 port @19:55:23.99 CSV.dial :flush @19:55:24.51 CS.seek 5ml @19:55:38.10 delay 5 seconds @19:55:42.49 delay 5 seconds @19:55:43.12
CSV.dial :air @19:55:43.81 CS.seek 7ml @19:55:47.51 ATV.dial ASV @19:55:48.50 ASV.dial :wasteR @19:55:48.87 AS.seek 0.3ml @19:55:50.28 CSV.dial CTV @19:55:50.31
CTV.dial CSV @19:55:51.86 CBV.dial :waste1 @19:55:52.39 CS.seek 0ml @19:56:11.07 Flushing lower manifold's waste2b port @19:56:11.21 CSV.dial :flush @19:56:12.04 CS.seek 5ml @19:56:25.87 delay 5 seconds @19:56:30.90 CSV.dial :air @19:56:31.49 CS.seek 6.999ml @19:56:33.39 delay 20 seconds @19:56:37.72 CSV.dial CTV @19:56:37.74
CTV.dial CSV @19:56:38.25 CBV.dial :waste2b @19:56:39.11 CS.seek 0ml @19:56:53.42 AS.seek 0.07ml @19:56:57.82
Garaging FlushPuck in Collection Clamp @19:56:57.90 Elbow.reconfigure TightElbow @19:56:57.95 Elbow.seek CC @19:56:58.21 Elbow.reconfigure LooseElbow @19:56:58.31 Forearm.seek CC @19:56:59.30 Hand.close @19:57:00.03 CC.seek :open @19:57:04.46 Forearm.seek :retract @19:57:05.47 Elbow.reconfigure TightElbow @19:57:05.52 Elbow.seek :garage @19:57:08.53 Elbow.reconfigure LooseElbow @19:57:09.17 Forearm.reconfigure TightForearm @19:57:09.22 Forearm.seek :garage @19:57:10.40 Hand.open @19:57:11.22 Forearm.reconfigure LooseForearm @19:57:11.27 Forearm.seek :retract @19:57:12.34 Starting finalPurge @19:57:12.35 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:57:12.43 Hand.open @19:57:12.72 Elbow.reconfigure TightElbow @19:57:12.77 Elbow.seek FlushPuck::Garage @19:57:12.99 Elbow.reconfigure LooseElbow @19:57:13.10 Forearm.seek :garage @19:57:14.15 Hand.close @19:57:15.04 Forearm.seek :retract @19:57:16.08 Elbow.reconfigure TightElbow @19:57:16.13 Elbow.seek CC @19:57:19.07 Elbow.reconfigure LooseElbow @19:57:19.18 Forearm.reconfigure TightForearm @19:57:19.23 Forearm.seek CC @19:57:20.58 CC.seek :closed @19:57:24.86 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.34 @19:57:24.93 Hand.open @19:57:25.72 Forearm.reconfigure LooseForearm @19:57:25.77 Forearm.seek :retract @19:57:26.76 Flushing the Collection Valve::Manifold @19:57:26.90 CSV.dial CSR @19:57:27.58 Pulling 5ml of CSR.flush @19:57:27.59 CSR.select :flush @19:57:27.72 CS.reconfigure CSconfig @19:57:27.77 CS.seek 5ml @19:57:39.93 delay 20 seconds @19:57:41.66
delay 5 seconds @19:57:46.68 CSR.select :air @19:57:46.84 CS.reconfigure CSfast @19:57:46.89 CS.seek 7.501ml @19:57:54.27 CSV.dial :waste @19:57:55.74 CS.reconfigure CSconfig @19:57:55.79 CS.seek 0ml @19:57:59.95 ATV.dial :waste2 @19:58:00.72 AS.reconfigure ASconfig @19:58:00.77 AS.seek :empty @19:58:05.15 Elution @19:58:05.16 ATV.dial :air1 @19:58:06.29 AS.reconfigure ASslow1 @19:58:06.34 AS.seek 0.049ml @19:58:15.94
Flushing lower manifold's waste1 port @19:58:16.07 CSV.dial :flush @19:58:16.58 CS.reconfigure CSfast @19:58:16.63 CS.seek 5ml @19:58:16.72 delay 3 seconds @19:58:19.73 ATV.dial :ElutionWater @19:58:20.42 AS.seek 0.109ml @19:58:30.35
delay 5 seconds @19:58:32.74 delay 3 seconds @19:58:35.37
CSV.dial :air @19:58:35.76 ATV.dial ASV,via: :air2 @19:58:36.06
CS.seek 7.001ml @19:58:37.86 ASV.dial :SPEelution @19:58:38.46 AFV.select :carrierSolution @19:58:38.63 AS.reconfigure ASnorm @19:58:38.68 AS.seek 0.759ml @19:58:42.13 CSV.dial CTV @19:58:42.16
CTV.dial CSV @19:58:42.80 CBV.dial :waste1 @19:58:44.18 CS.seek 0ml @19:59:03.19 Flushing lower manifold's waste2b port @19:59:03.33 CSV.dial :flush @19:59:04.27 CS.seek 5ml @19:59:11.72 delay 3 seconds @19:59:14.74 AFV.select :holdingCoil @19:59:14.91 AS.reconfigure ASslow1 @19:59:14.96 AS.seek 0.474ml @19:59:17.85
delay 5 seconds @19:59:22.88 CSV.dial :air @19:59:23.45 CS.seek 7ml @19:59:29.44 CSV.dial CTV @19:59:29.47
CTV.dial CSV @19:59:29.97 CBV.dial :waste2b @19:59:30.83 CS.seek 0ml @19:59:49.50 Disinfecting external Intake with kill @19:59:49.64 CSV.dial CSR @19:59:50.19 Pulling 8ml of CSR.kill @19:59:50.20 CSR.select :kill @19:59:50.33 CS.seek 8ml @20:00:11.72 delay 5 seconds @20:00:12.52 delay 5 seconds @20:00:16.74
CSR.select :air @20:00:16.88 CS.seek 10ml @20:00:17.60 AS.reconfigure ASslow2 @20:00:17.66 AS.seek 0.439ml @20:00:22.94 CSV.dial CBV @20:00:22.96
CBV.dial CSV @20:00:24.28 CTV.dial :intake @20:00:25.15 Intake.select :open @20:00:25.27 CS.seek 2ml @20:00:47.06 Intake.select :close @20:00:47.18 CTV.dial :air @20:00:48.02 CS.reconfigure CSconfig @20:00:48.07 CS.seek 0ml @20:00:53.07 delay 1:00 @20:00:54.13
CSV.dial CSR @20:00:55.33 Pulling 5ml of CSR.flush @20:00:55.34 CSR.select :flush @20:00:55.47 CS.seek 5ml @20:01:09.29 delay 5 seconds @20:01:14.31 CSR.select :air @20:01:14.44 CS.seek 10ml @20:01:28.15 CSV.dial CBV @20:01:29.31 CS.seek 0ml @20:01:53.09 pushing eluate to holding coil @20:01:53.16 AS.seek 0.279ml @20:01:55.88
Garaging FlushPuck in Collection Clamp @20:01:55.96 Elbow.reconfigure TightElbow @20:01:56.01 Elbow.seek CC @20:01:56.28 Elbow.reconfigure LooseElbow @20:01:56.38 Forearm.seek CC @20:01:57.37 Hand.close @20:01:58.18 CC.seek :open @20:02:02.57 Forearm.seek :retract @20:02:03.57 Elbow.reconfigure TightElbow @20:02:03.62 Elbow.seek :garage @20:02:06.77 Elbow.reconfigure LooseElbow @20:02:06.88 Forearm.reconfigure TightForearm @20:02:06.93 Forearm.seek :garage @20:02:08.10 Hand.open @20:02:08.99 Forearm.reconfigure LooseForearm @20:02:09.04 Forearm.seek :retract @20:02:10.07 Completed [mbaPhase] begun 17:47:07 @20:02:10.09 Extending 18tun1 until completion of MFBPCR @20:04:34.25 delay 30 seconds @20:05:04.28 ATV.dial :waste,via: :air3 @20:05:06.00 AS.reconfigure ASconfig @20:05:06.05 AS.seek :empty @20:05:22.46 Parking Bleach in LysateSource line and SPEcolumn @20:05:22.47 ATV.dial :air3 @20:05:23.32 AS.reconfigure ASslow1 @20:05:23.37 AS.seek 0.029ml @20:05:29.71 ATV.dial :ElutionWater @20:05:30.32 AS.seek 0.059ml @20:05:36.62 ATV.dial :air3 @20:05:37.24 AS.seek 0.069ml @20:05:39.57 delay 2 seconds @20:05:41.59 ATV.dial :bleach @20:05:42.09 AS.seek 0.769ml @20:08:03.17 delay 3 seconds @20:08:06.18 ATV.dial :air3 @20:08:06.74 delay 3 seconds @20:08:09.83 AS.seek 0.794ml @20:08:15.12 delay 3 seconds @20:08:18.14 ATV.dial :bleach @20:08:18.78 AS.seek 0.779ml @20:08:22.13 delay 3 seconds @20:08:25.15 ATV.dial :AIV @20:08:26.41 AS.reconfigure ASconfig @20:08:26.46 AS.seek 0.015ml @20:09:10.68 ATV.dial :waste @20:09:12.19 AS.seek :empty @20:09:13.41 ATV.dial :air3 @20:09:14.10 AS.reconfigure ASslow1 @20:09:14.15 AS.seek 0.009ml @20:09:16.46 delay 2 seconds @20:09:18.47 ATV.dial :bleach @20:09:19.03 AS.seek 0.579ml @20:11:13.93 delay 3 seconds @20:11:16.95 ATV.dial :air3 @20:11:17.55 delay 3 seconds @20:11:20.64 AS.seek 0.604ml @20:11:26.01 delay 3 seconds @20:11:29.03 ATV.dial :bleach @20:11:29.67 AS.seek 0.589ml @20:11:32.99 delay 3 seconds @20:11:36.02 ATV.dial :ASV @20:11:37.14 ASV.dial :wasteR @20:11:37.73 AS.seek 0ml @20:13:36.49 delay 1:00 @20:14:36.51 AFV.select :carrierSolution @20:14:36.62 delay 2 seconds @20:14:38.65 AS.reconfigure ASconfig @20:14:38.70 AS.seek 1ml @20:15:36.46 delay 1 second @20:15:37.47 AFV.select :holdingCoil @20:15:37.58 delay 1 second @20:15:38.60 ATV.dial ASV @20:15:38.62 ASV.dial :wasteR @20:15:38.64 AS.reconfigure ASslow1 @20:15:38.68 AS.seek 0ml @20:19:00.05 delay 20 seconds @20:19:20.07 ATV.dial :air3 @20:19:21.59 AS.seek 0.59ml @20:21:20.53 delay 3 seconds @20:21:23.55 ATV.dial :ASV @20:21:24.97 ASV.dial :wasteR @20:21:25.06 AS.seek 0.09ml @20:23:05.91 delay 30 seconds @20:23:35.93 ATV.dial :extSrc @20:23:36.69 delay 3 seconds @20:23:39.71 ATV.dial :ElutionWater @20:23:40.83 AS.seek 0.12ml @20:23:47.16 ATV.dial :air3 @20:23:47.79 AS.seek 0.13ml @20:23:50.08 delay 2 seconds @20:23:52.09 ATV.dial :bleach @20:23:52.57 AS.seek 0.23ml @20:24:12.94 delay 3 seconds @20:24:15.96 ATV.dial :air3 @20:24:16.52 delay 3 seconds @20:24:19.61 AS.seek 0.255ml @20:24:24.84 delay 3 seconds @20:24:27.86 ATV.dial :bleach @20:24:28.49 AS.seek 0.24ml @20:24:31.80 delay 3 seconds @20:24:34.82 ATV.dial :ASV @20:24:35.86 ASV.dial :SPEdelivery @20:24:36.24 AFV.select :carrierSolution @20:24:36.36 delay 2 seconds @20:24:38.39 AS.reconfigure ASconfig @20:24:38.44 AS.seek 0.5ml @20:24:53.69 delay 1 second @20:24:54.70 AFV.select :holdingCoil @20:24:54.86 AS.reconfigure ASslow1 @20:24:54.91 AS.seek 0.17ml @20:26:01.51 SPE.hold 2:46:40,95C @20:26:01.57 delay 3:00 @20:29:01.60 AFV.select :carrierSolution @20:29:01.71 delay 2 seconds @20:29:03.74 AS.reconfigure ASconfig @20:29:03.79 AS.seek 1ml @20:29:51.80 delay 1 second @20:29:52.82 AFV.select :holdingCoil @20:29:52.93 delay 1 second @20:29:53.95 ASV.dial :wasteD @20:29:55.06 AS.seek 0ml @20:30:52.84 Priming Eluate @20:30:52.85 AFV.select :carrierSolution @20:30:52.96 delay 2 seconds @20:30:54.99 AS.seek 0.5ml @20:31:24.53 delay 1 second @20:31:25.54 AFV.select :holdingCoil @20:31:25.65 ATV.dial ASV @20:31:25.66 ASV.dial :SPErecovery @20:31:26.27 Swish #1 of 2 @20:31:26.36 AS.reconfigure ASslow0 @20:31:26.41 AS.seek 0.6ml @20:31:36.91 AS.seek 0.5ml @20:31:47.29 Swish #2 of 2 @20:31:47.38 AS.seek 0.6ml @20:31:57.81 AS.seek 0.5ml @20:32:08.22 delay 3 seconds @20:32:11.31 AS.reconfigure ASslow1 @20:32:11.36 AS.seek 0.684ml @20:32:48.67 delay 3 seconds @20:32:51.70 Eluate primed @20:32:51.71 ATV.dial :waste2 @20:32:52.33 AS.reconfigure ASnorm @20:32:52.39 AS.seek 0ml @20:33:27.21 SPE.coast @20:33:27.29 ASV.dial :SPErecovery @20:33:27.32 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep0920h1500ml.pcr @20:33:27.33 Building PCR Slug from ASV with MM2 mix, PP4 primer @20:33:27.35 Priming with carrier @20:33:27.36 ATV.dial ARV @20:33:28.32 ARV.dial :carrier @20:33:28.70 AS.seek 0.5ml @20:33:54.21 AFV.select :carrierSolution @20:33:54.33 delay 2 seconds @20:33:56.36 AS.reconfigure ASconfig @20:33:56.41 AS.seek 1ml @20:34:25.47 delay 1 second @20:34:26.48 AFV.select :holdingCoil @20:34:26.59 delay 1 second @20:34:27.61 ATV.dial :waste @20:34:29.13 AS.reconfigure ASnorm @20:34:29.18 AS.seek :empty @20:35:19.91 AFV.select :carrierSolution @20:35:20.03 delay 2 seconds @20:35:22.06 AS.reconfigure ASconfig @20:35:22.11 AS.seek 0.5ml @20:35:51.22 delay 1 second @20:35:52.23 AFV.select :holdingCoil @20:35:52.34 Building Slug @20:35:52.35 ATV.dial :ARV @20:35:54.06 delay 2 seconds @20:35:56.08 ARV.dial :air1 @20:35:56.50 delay 2 seconds @20:35:58.59 AS.reconfigure ASslow2 @20:35:58.64 AS.seek 0.506ml @20:36:05.25 delay 2 seconds @20:36:07.27 ARV.dial :MM2 @20:36:08.78 delay 2 seconds @20:36:10.81 Adding MM2 @20:36:10.89 AS.seek 0.524ml @20:36:29.24 delay 25 seconds @20:36:54.26 ARV.dial :PP4 @20:36:54.67 Adding PP4 @20:36:54.69 delay 2 seconds @20:36:56.77 AS.seek 0.53ml @20:37:03.21 delay 10 seconds @20:37:13.24 ARV.dial :air3 @20:37:13.63 adding ARV air @20:37:13.65 delay 2 seconds @20:37:15.74 AS.seek 0.536ml @20:37:22.02 delay 5 seconds @20:37:27.04 ARV.dial :carrier @20:37:28.34 delay 7 seconds @20:37:35.42 AS.seek 0.594ml @20:38:34.09 delay 5 seconds @20:38:39.11 ATV.dial :ASV @20:38:39.53 delay 2 seconds @20:38:41.62 AS.seek 0.6ml @20:38:47.86 delay 4 seconds @20:38:51.88 ATV.dial ARV @20:38:52.42 delay 2 seconds @20:38:54.44 ARV.dial :carrier @20:38:54.46 delay 2 seconds @20:38:56.55 AS.seek 0.65ml @20:39:47.09 delay 4 seconds @20:39:51.11 ATV.dial PCR @20:39:51.72 ASV.dial :waste1 @20:39:52.50 ARV.dial :air1 @20:39:52.91 Positioning Slug by 0.324ml @20:39:53.00 AS.seek 0.326ml @20:45:18.82 delay 5 seconds @20:45:23.85 ATV.dial ARV @20:45:24.62 ASV.dial :PCRlock @20:45:25.00 delay 5 seconds @20:45:30.57 Running Tt1 Profile60C300 thermocycling @20:45:30.59 PCR.program Profile60C300 @20:45:30.72 Profile60C300 should complete in < 1:45:21 @20:45:30.73 PCR.run 2:16:41.25 @22:10:24.29 PCR.off @22:10:26.07 PCR for Tt1 completed -- closed /var/log/esp/Tt118sep0920h1500ml.pcr @22:10:26.14 Cleaning PCR @22:10:26.15 AFV.select :carrierSolution @22:10:26.26 delay 2 seconds @22:10:28.29 AS.reconfigure ASconfig @22:10:28.34 AS.seek 0.5ml @22:10:38.65 delay 1 second @22:10:39.67 AFV.select :holdingCoil @22:10:39.78 ASV.dial :waste1 @22:10:40.17 ATV.dial :air3 @22:10:41.46 AS.reconfigure ASslow1 @22:10:41.51 AS.seek 0.51ml @22:10:43.93 delay 2 seconds @22:10:45.94 ATV.dial :bleach @22:10:46.57 AS.seek 0.81ml @22:11:47.20 delay 3 seconds @22:11:50.22 ATV.dial :air3 @22:11:50.78 delay 3 seconds @22:11:53.87 AS.seek 0.835ml @22:11:59.10 delay 3 seconds @22:12:02.12 ATV.dial :bleach @22:12:02.75 AS.seek 0.82ml @22:12:06.06 delay 3 seconds @22:12:09.08 ARV.dial :waste @22:12:09.70 delay 3 seconds @22:12:12.72 ATV.dial :ARV @22:12:13.61 AS.reconfigure ASconfig @22:12:13.66 AS.seek 0.47ml @22:12:34.06 AFV.select :carrierSolution @22:12:34.18 delay 2 seconds @22:12:36.21 AS.seek 1ml @22:13:06.96 delay 1 second @22:13:07.97 AFV.select :holdingCoil @22:13:08.08 delay 1 second @22:13:09.10 AS.reconfigure ASfast @22:13:09.15 AS.seek 0ml @22:13:36.50 AFV.select :carrierSolution @22:13:36.62 delay 2 seconds @22:13:38.65 AS.reconfigure ASconfig @22:13:38.70 AS.seek 1ml @22:14:36.45 delay 1 second @22:14:37.46 AFV.select :holdingCoil @22:14:37.57 delay 1 second @22:14:38.60 AS.reconfigure ASfast @22:14:38.65 AS.seek 0ml @22:15:05.98 AFV.select :carrierSolution @22:15:06.10 delay 2 seconds @22:15:08.13 AS.reconfigure ASconfig @22:15:08.18 AS.seek 1ml @22:16:05.95 delay 1 second @22:16:06.96 AFV.select :holdingCoil @22:16:07.07 delay 1 second @22:16:08.10 AS.reconfigure ASfast @22:16:08.15 AS.seek 0ml @22:16:35.57 ARV.dial between carrier and air1 @22:16:36.07 ATV.dial :air3 @22:16:37.38 AS.reconfigure ASslow1 @22:16:37.43 AS.seek 0.01ml @22:16:39.77 delay 2 seconds @22:16:41.79 ATV.dial :bleach @22:16:42.42 AS.seek 0.81ml @22:19:23.62 delay 3 seconds @22:19:26.64 ATV.dial :air3 @22:19:27.22 delay 3 seconds @22:19:30.31 AS.seek 0.835ml @22:19:35.63 delay 3 seconds @22:19:38.65 ATV.dial :bleach @22:19:39.28 AS.seek 0.82ml @22:19:42.53 delay 3 seconds @22:19:45.55 ATV.dial PCR @22:19:45.95 AS.reconfigure ASconfig @22:19:46.00 AS.seek :empty @22:20:33.47 PCR.program :bleachProfile @22:20:33.54 PCR program should complete in < 2:45 @22:20:33.55 PCR.run 10:00 @22:23:29.29 PCR.off @22:23:29.32 AFV.select :carrierSolution @22:23:29.43 delay 2 seconds @22:23:31.46 AS.seek 1ml @22:24:29.31 delay 1 second @22:24:30.32 AFV.select :holdingCoil @22:24:30.91 delay 1 second @22:24:32.01 AS.reconfigure ASfast @22:24:32.06 AS.seek 0ml @22:24:59.42 delay 3 seconds @22:25:02.44 AFV.select :carrierSolution @22:25:02.55 delay 2 seconds @22:25:04.58 AS.reconfigure ASconfig @22:25:04.63 AS.seek 1ml @22:26:02.43 delay 1 second @22:26:03.44 AFV.select :holdingCoil @22:26:03.55 delay 1 second @22:26:04.58 AS.reconfigure ASfast @22:26:04.63 AS.seek 0ml @22:26:31.98 delay 3 seconds @22:26:35.00 AFV.select :carrierSolution @22:26:35.11 delay 2 seconds @22:26:37.14 AS.reconfigure ASconfig @22:26:37.20 AS.seek 1ml @22:27:34.96 delay 1 second @22:27:35.97 AFV.select :holdingCoil @22:27:36.08 delay 1 second @22:27:37.11 AS.reconfigure ASfast @22:27:37.16 AS.seek 0ml @22:28:04.51 delay 3 seconds @22:28:07.53 AFV.select :carrierSolution @22:28:07.64 delay 2 seconds @22:28:09.67 AS.reconfigure ASconfig @22:28:09.72 AS.seek 1ml @22:29:07.53 delay 1 second @22:29:08.54 AFV.select :holdingCoil @22:29:08.65 delay 1 second @22:29:09.68 AS.reconfigure ASfast @22:29:09.73 AS.seek 0ml @22:29:37.06 delay 3 seconds @22:29:40.08 AFV.select :carrierSolution @22:29:40.19 delay 2 seconds @22:29:42.22 AS.reconfigure ASconfig @22:29:42.27 AS.seek 1ml @22:30:40.03 delay 1 second @22:30:41.04 AFV.select :holdingCoil @22:30:41.15 delay 1 second @22:30:42.18 AS.reconfigure ASnorm @22:30:42.23 AS.seek 0ml @22:31:32.94 Setting up PCR for Rp -- writing /var/log/esp/Rp18sep0922h1500ml.pcr @22:31:32.96 Building PCR Slug from ASV with MM2 mix, PP3 primer @22:31:32.97 Priming with carrier @22:31:32.98 ATV.dial ARV @22:31:33.59 ARV.dial :carrier @22:31:33.95 AS.seek 0.5ml @22:31:59.52 AFV.select :carrierSolution @22:31:59.64 delay 2 seconds @22:32:01.67 AS.reconfigure ASconfig @22:32:01.72 AS.seek 1ml @22:32:30.73 delay 1 second @22:32:31.74 AFV.select :holdingCoil @22:32:31.85 delay 1 second @22:32:32.87 ATV.dial :waste @22:32:34.53 AS.reconfigure ASnorm @22:32:34.58 AS.seek :empty @22:33:25.28 AFV.select :carrierSolution @22:33:25.40 delay 2 seconds @22:33:27.43 AS.reconfigure ASconfig @22:33:27.48 AS.seek 0.5ml @22:33:56.59 delay 1 second @22:33:57.60 AFV.select :holdingCoil @22:33:57.71 Building Slug @22:33:57.72 ATV.dial :ARV @22:33:59.34 delay 2 seconds @22:34:01.36 ARV.dial :air1 @22:34:01.75 delay 2 seconds @22:34:03.83 AS.reconfigure ASslow2 @22:34:03.89 AS.seek 0.506ml @22:34:10.52 delay 2 seconds @22:34:12.54 ARV.dial :MM2 @22:34:14.06 delay 2 seconds @22:34:16.09 Adding MM2 @22:34:16.17 AS.seek 0.524ml @22:34:34.40 delay 25 seconds @22:34:59.43 ARV.dial :PP3 @22:34:59.82 Adding PP3 @22:34:59.83 delay 2 seconds @22:35:01.92 AS.seek 0.53ml @22:35:08.18 delay 10 seconds @22:35:18.20 ARV.dial :air2 @22:35:18.60 adding ARV air @22:35:18.61 delay 2 seconds @22:35:20.70 AS.seek 0.536ml @22:35:27.12 delay 5 seconds @22:35:32.14 ARV.dial :carrier @22:35:33.45 delay 7 seconds @22:35:40.54 AS.seek 0.594ml @22:36:39.26 delay 5 seconds @22:36:44.28 ATV.dial :ASV @22:36:44.68 delay 2 seconds @22:36:46.76 AS.seek 0.6ml @22:36:53.29 delay 4 seconds @22:36:57.31 ATV.dial ARV @22:36:57.85 delay 2 seconds @22:36:59.87 ARV.dial :carrier @22:36:59.89 delay 2 seconds @22:37:01.97 AS.seek 0.65ml @22:37:52.63 delay 4 seconds @22:37:56.65 ATV.dial PCR @22:37:57.26 ASV.dial :waste1 @22:37:57.27 ARV.dial :air1 @22:37:57.77 Positioning Slug by 0.324ml @22:37:58.41 AS.seek 0.326ml @22:43:24.57 delay 5 seconds @22:43:29.59 ATV.dial ARV @22:43:30.37 ASV.dial :PCRlock @22:43:30.76 delay 5 seconds @22:43:35.79 Running Rp Profile60C300 thermocycling @22:43:35.80 PCR.program Profile60C300 @22:43:35.94 Profile60C300 should complete in < 1:45:21 @22:43:35.95 PCR.run 2:16:41.25 @00:08:13.47CEST10-Sep-18 PCR.off @00:08:15.45 PCR for Rp completed -- closed /var/log/esp/Rp18sep0922h1500ml.pcr @00:08:15.53 Cleaning PCR @00:08:15.54 AFV.select :carrierSolution @00:08:15.65 delay 2 seconds @00:08:17.68 AS.reconfigure ASconfig @00:08:17.73 AS.seek 0.5ml @00:08:28.05 delay 1 second @00:08:29.06 AFV.select :holdingCoil @00:08:29.17 ASV.dial :waste1 @00:08:29.55 ATV.dial :air3 @00:08:30.83 AS.reconfigure ASslow1 @00:08:30.89 AS.seek 0.51ml @00:08:33.19 delay 2 seconds @00:08:35.21 ATV.dial :bleach @00:08:35.82 AS.seek 0.81ml @00:09:36.52 delay 3 seconds @00:09:39.54 ATV.dial :air3 @00:09:40.08 delay 3 seconds @00:09:43.17 AS.seek 0.835ml @00:09:48.49 delay 3 seconds @00:09:51.51 ATV.dial :bleach @00:09:52.14 AS.seek 0.82ml @00:09:55.57 delay 3 seconds @00:09:58.59 ARV.dial :waste @00:09:59.19 delay 3 seconds @00:10:02.21 ATV.dial :ARV @00:10:03.11 AS.reconfigure ASconfig @00:10:03.16 AS.seek 0.47ml @00:10:23.59 AFV.select :carrierSolution @00:10:23.71 delay 2 seconds @00:10:25.74 AS.seek 1ml @00:10:56.52 delay 1 second @00:10:57.53 AFV.select :holdingCoil @00:10:57.64 delay 1 second @00:10:58.67 AS.reconfigure ASfast @00:10:58.72 AS.seek 0ml @00:11:26.09 AFV.select :carrierSolution @00:11:26.21 delay 2 seconds @00:11:28.24 AS.reconfigure ASconfig @00:11:28.29 AS.seek 1ml @00:12:26.04 delay 1 second @00:12:27.06 AFV.select :holdingCoil @00:12:27.17 delay 1 second @00:12:28.20 AS.reconfigure ASfast @00:12:28.25 AS.seek 0ml @00:12:55.73 AFV.select :carrierSolution @00:12:55.85 delay 2 seconds @00:12:57.88 AS.reconfigure ASconfig @00:12:57.93 AS.seek 1ml @00:13:55.69 delay 1 second @00:13:56.70 AFV.select :holdingCoil @00:13:56.81 delay 1 second @00:13:57.84 AS.reconfigure ASfast @00:13:57.89 AS.seek 0ml @00:14:25.23 ARV.dial between carrier and air1 @00:14:25.74 ATV.dial :air3 @00:14:27.03 AS.reconfigure ASslow1 @00:14:27.08 AS.seek 0.01ml @00:14:29.38 delay 2 seconds @00:14:31.39 ATV.dial :bleach @00:14:32.01 AS.seek 0.81ml @00:17:13.24 delay 3 seconds @00:17:16.26 ATV.dial :air3 @00:17:16.82 delay 3 seconds @00:17:19.91 AS.seek 0.835ml @00:17:25.24 delay 3 seconds @00:17:28.26 ATV.dial :bleach @00:17:28.89 AS.seek 0.82ml @00:17:32.14 delay 3 seconds @00:17:35.16 ATV.dial PCR @00:17:35.57 AS.reconfigure ASconfig @00:17:35.62 AS.seek :empty @00:18:23.10 PCR.program :bleachProfile @00:18:23.17 PCR program should complete in < 2:45 @00:18:23.18 PCR.run 10:00 @00:21:15.91 PCR.off @00:21:15.93 AFV.select :carrierSolution @00:21:16.04 delay 2 seconds @00:21:18.07 AS.seek 1ml @00:22:15.89 delay 1 second @00:22:16.90 AFV.select :holdingCoil @00:22:17.01 delay 1 second @00:22:18.04 AS.reconfigure ASfast @00:22:18.65 AS.seek 0ml @00:22:45.99 delay 3 seconds @00:22:49.01 AFV.select :carrierSolution @00:22:49.12 delay 2 seconds @00:22:51.15 AS.reconfigure ASconfig @00:22:51.22 AS.seek 1ml @00:23:49.02 delay 1 second @00:23:50.04 AFV.select :holdingCoil @00:23:50.15 delay 1 second @00:23:51.18 AS.reconfigure ASfast @00:23:51.23 AS.seek 0ml @00:24:18.58 delay 3 seconds @00:24:21.60 AFV.select :carrierSolution @00:24:21.71 delay 2 seconds @00:24:23.74 AS.reconfigure ASconfig @00:24:23.80 AS.seek 1ml @00:25:21.55 delay 1 second @00:25:22.57 AFV.select :holdingCoil @00:25:22.67 delay 1 second @00:25:23.69 AS.reconfigure ASfast @00:25:23.74 AS.seek 0ml @00:25:51.10 delay 3 seconds @00:25:54.12 AFV.select :carrierSolution @00:25:54.23 delay 2 seconds @00:25:56.26 AS.reconfigure ASconfig @00:25:56.31 AS.seek 1ml @00:26:54.14 delay 1 second @00:26:55.15 AFV.select :holdingCoil @00:26:55.26 delay 1 second @00:26:56.29 AS.reconfigure ASfast @00:26:56.34 AS.seek 0ml @00:27:23.69 delay 3 seconds @00:27:26.71 AFV.select :carrierSolution @00:27:26.82 delay 2 seconds @00:27:28.85 AS.reconfigure ASconfig @00:27:28.90 AS.seek 1ml @00:28:26.75 delay 1 second @00:28:27.77 AFV.select :holdingCoil @00:28:27.88 delay 1 second @00:28:28.90 AS.reconfigure ASnorm @00:28:28.95 AS.seek 0ml @00:29:19.68 Setting up PCR for Ss -- writing /var/log/esp/Ss18sep1000h1500ml.pcr @00:29:19.70 Building PCR Slug from ASV with MM1 mix, PP2 primer @00:29:19.71 Priming with carrier @00:29:19.72 ATV.dial ARV @00:29:20.33 ARV.dial :carrier @00:29:20.68 AS.seek 0.5ml @00:29:46.24 AFV.select :carrierSolution @00:29:46.36 delay 2 seconds @00:29:48.39 AS.reconfigure ASconfig @00:29:48.44 AS.seek 1ml @00:30:17.46 delay 1 second @00:30:18.48 AFV.select :holdingCoil @00:30:18.59 delay 1 second @00:30:19.61 ATV.dial :waste @00:30:21.27 AS.reconfigure ASnorm @00:30:21.32 AS.seek :empty @00:31:12.02 AFV.select :carrierSolution @00:31:12.14 delay 2 seconds @00:31:14.17 AS.reconfigure ASconfig @00:31:14.22 AS.seek 0.5ml @00:31:43.33 delay 1 second @00:31:44.34 AFV.select :holdingCoil @00:31:44.45 Building Slug @00:31:44.46 ATV.dial :ARV @00:31:46.18 delay 2 seconds @00:31:48.20 ARV.dial :air1 @00:31:48.61 delay 2 seconds @00:31:50.69 AS.reconfigure ASslow2 @00:31:50.75 AS.seek 0.506ml @00:31:57.13 delay 2 seconds @00:31:59.14 ARV.dial :MM1 @00:31:59.75 delay 2 seconds @00:32:01.79 Adding MM1 @00:32:01.86 AS.seek 0.524ml @00:32:20.13 delay 25 seconds @00:32:45.15 ARV.dial :PP2 @00:32:45.55 Adding PP2 @00:32:45.56 delay 2 seconds @00:32:47.65 AS.seek 0.53ml @00:32:54.08 delay 10 seconds @00:33:04.10 ARV.dial :air2 @00:33:04.49 adding ARV air @00:33:04.50 delay 2 seconds @00:33:06.59 AS.seek 0.536ml @00:33:12.92 delay 5 seconds @00:33:17.94 ARV.dial :carrier @00:33:19.24 delay 7 seconds @00:33:26.33 AS.seek 0.594ml @00:34:25.02 delay 5 seconds @00:34:30.04 ATV.dial :ASV @00:34:30.46 delay 2 seconds @00:34:32.54 AS.seek 0.6ml @00:34:38.82 delay 4 seconds @00:34:42.84 ATV.dial ARV @00:34:43.38 delay 2 seconds @00:34:45.40 ARV.dial :carrier @00:34:45.42 delay 2 seconds @00:34:47.50 AS.seek 0.65ml @00:35:37.91 delay 4 seconds @00:35:41.94 ATV.dial PCR @00:35:42.57 ASV.dial :waste1 @00:35:42.58 ARV.dial :air1 @00:35:43.09 Positioning Slug by 0.324ml @00:35:43.18 AS.seek 0.326ml @00:41:09.24 delay 5 seconds @00:41:14.27 ATV.dial ARV @00:41:15.16 ASV.dial :PCRlock @00:41:15.55 delay 5 seconds @00:41:20.58 Running Ss Profile60C350 thermocycling @00:41:20.59 PCR.program Profile60C350 @00:41:20.74 Profile60C350 should complete in < 1:48:05 @00:41:20.75 PCR.run 2:20:06.25 @02:06:19.12 PCR.off @02:06:21.46 PCR for Ss completed -- closed /var/log/esp/Ss18sep1000h1500ml.pcr @02:06:21.53 Cleaning PCR @02:06:21.55 AFV.select :carrierSolution @02:06:21.66 delay 2 seconds @02:06:23.69 AS.reconfigure ASconfig @02:06:23.74 AS.seek 0.5ml @02:06:34.04 delay 1 second @02:06:35.06 AFV.select :holdingCoil @02:06:35.17 ASV.dial :waste1 @02:06:35.54 ATV.dial :air3 @02:06:36.82 AS.reconfigure ASslow1 @02:06:36.87 AS.seek 0.51ml @02:06:39.28 delay 2 seconds @02:06:41.30 ATV.dial :bleach @02:06:41.91 AS.seek 0.81ml @02:07:42.53 delay 3 seconds @02:07:45.55 ATV.dial :air3 @02:07:46.09 delay 3 seconds @02:07:49.18 AS.seek 0.835ml @02:07:54.39 delay 3 seconds @02:07:57.41 ATV.dial :bleach @02:07:58.04 AS.seek 0.82ml @02:08:01.37 delay 3 seconds @02:08:04.39 ARV.dial :waste @02:08:05.00 delay 3 seconds @02:08:08.02 ATV.dial :ARV @02:08:08.91 AS.reconfigure ASconfig @02:08:08.96 AS.seek 0.47ml @02:08:29.36 AFV.select :carrierSolution @02:08:29.48 delay 2 seconds @02:08:31.51 AS.seek 1ml @02:09:02.28 delay 1 second @02:09:03.29 AFV.select :holdingCoil @02:09:03.40 delay 1 second @02:09:04.43 AS.reconfigure ASfast @02:09:04.48 AS.seek 0ml @02:09:31.94 AFV.select :carrierSolution @02:09:32.06 delay 2 seconds @02:09:34.09 AS.reconfigure ASconfig @02:09:34.14 AS.seek 1ml @02:10:31.94 delay 1 second @02:10:32.95 AFV.select :holdingCoil @02:10:33.06 delay 1 second @02:10:34.08 AS.reconfigure ASfast @02:10:34.13 AS.seek 0ml @02:11:01.49 AFV.select :carrierSolution @02:11:01.61 delay 2 seconds @02:11:03.64 AS.reconfigure ASconfig @02:11:03.69 AS.seek 1ml @02:12:01.47 delay 1 second @02:12:02.48 AFV.select :holdingCoil @02:12:02.59 delay 1 second @02:12:03.62 AS.reconfigure ASfast @02:12:03.67 AS.seek 0ml @02:12:31.02 ARV.dial between carrier and air1 @02:12:31.53 ATV.dial :air3 @02:12:32.82 AS.reconfigure ASslow1 @02:12:32.87 AS.seek 0.01ml @02:12:35.23 delay 2 seconds @02:12:37.24 ATV.dial :bleach @02:12:37.85 AS.seek 0.81ml @02:15:19.11 delay 3 seconds @02:15:22.13 ATV.dial :air3 @02:15:22.67 delay 3 seconds @02:15:25.76 AS.seek 0.835ml @02:15:31.07 delay 3 seconds @02:15:34.09 ATV.dial :bleach @02:15:34.72 AS.seek 0.82ml @02:15:38.03 delay 3 seconds @02:15:41.05 ATV.dial PCR @02:15:41.45 AS.reconfigure ASconfig @02:15:41.50 AS.seek :empty @02:16:28.96 PCR.program :bleachProfile @02:16:29.02 PCR program should complete in < 2:45 @02:16:29.03 PCR.run 10:00 @02:19:21.76 PCR.off @02:19:21.78 AFV.select :carrierSolution @02:19:21.89 delay 2 seconds @02:19:23.92 AS.seek 1ml @02:20:21.76 delay 1 second @02:20:23.24 AFV.select :holdingCoil @02:20:23.37 delay 1 second @02:20:24.40 AS.reconfigure ASfast @02:20:24.45 AS.seek 0ml @02:20:51.79 delay 3 seconds @02:20:54.81 AFV.select :carrierSolution @02:20:54.92 delay 2 seconds @02:20:56.95 AS.reconfigure ASconfig @02:20:57.01 AS.seek 1ml @02:21:54.81 delay 1 second @02:21:55.83 AFV.select :holdingCoil @02:21:55.94 delay 1 second @02:21:56.97 AS.reconfigure ASfast @02:21:57.02 AS.seek 0ml @02:22:24.39 delay 3 seconds @02:22:27.40 AFV.select :carrierSolution @02:22:27.51 delay 2 seconds @02:22:29.53 AS.reconfigure ASconfig @02:22:29.59 AS.seek 1ml @02:23:27.34 delay 1 second @02:23:28.35 AFV.select :holdingCoil @02:23:28.46 delay 1 second @02:23:29.49 AS.reconfigure ASfast @02:23:29.54 AS.seek 0ml @02:23:56.87 delay 3 seconds @02:23:59.89 AFV.select :carrierSolution @02:24:00.00 delay 2 seconds @02:24:02.03 AS.reconfigure ASconfig @02:24:02.09 AS.seek 1ml @02:24:59.93 delay 1 second @02:25:00.94 AFV.select :holdingCoil @02:25:01.05 delay 1 second @02:25:02.08 AS.reconfigure ASfast @02:25:02.14 AS.seek 0ml @02:25:29.51 delay 3 seconds @02:25:32.53 AFV.select :carrierSolution @02:25:32.64 delay 2 seconds @02:25:34.67 AS.reconfigure ASconfig @02:25:34.73 AS.seek 1ml @02:26:32.58 delay 1 second @02:26:33.59 AFV.select :holdingCoil @02:26:33.70 delay 1 second @02:26:34.73 AS.reconfigure ASnorm @02:26:34.78 AS.seek 0ml @02:27:25.50 Setting up PCR for Bb -- writing /var/log/esp/Bb18sep1002h1500ml.pcr @02:27:25.52 Building PCR Slug from ASV with MM3 mix, PP5 primer @02:27:25.53 Priming with carrier @02:27:25.54 ATV.dial ARV @02:27:26.25 ARV.dial :carrier @02:27:26.61 AS.seek 0.5ml @02:27:52.19 AFV.select :carrierSolution @02:27:52.31 delay 2 seconds @02:27:54.34 AS.reconfigure ASconfig @02:27:54.40 AS.seek 1ml @02:28:23.43 delay 1 second @02:28:24.45 AFV.select :holdingCoil @02:28:24.56 delay 1 second @02:28:25.57 ATV.dial :waste @02:28:27.23 AS.reconfigure ASnorm @02:28:27.28 AS.seek :empty @02:29:18.05 AFV.select :carrierSolution @02:29:18.17 delay 2 seconds @02:29:20.20 AS.reconfigure ASconfig @02:29:20.25 AS.seek 0.5ml @02:29:49.31 delay 1 second @02:29:50.32 AFV.select :holdingCoil @02:29:50.43 Building Slug @02:29:50.44 ATV.dial :ARV @02:29:52.06 delay 2 seconds @02:29:54.08 ARV.dial :air1 @02:29:54.49 delay 2 seconds @02:29:56.57 AS.reconfigure ASslow2 @02:29:56.63 AS.seek 0.506ml @02:30:02.91 delay 2 seconds @02:30:04.93 ARV.dial :MM3 @02:30:06.01 delay 2 seconds @02:30:08.04 Adding MM3 @02:30:08.12 AS.seek 0.524ml @02:30:26.39 delay 25 seconds @02:30:51.41 ARV.dial :PP5 @02:30:51.81 Adding PP5 @02:30:51.82 delay 2 seconds @02:30:53.91 AS.seek 0.53ml @02:31:00.06 delay 10 seconds @02:31:10.08 ARV.dial :air3 @02:31:10.47 adding ARV air @02:31:10.48 delay 2 seconds @02:31:12.56 AS.seek 0.536ml @02:31:18.95 delay 5 seconds @02:31:23.97 ARV.dial :carrier @02:31:25.26 delay 7 seconds @02:31:32.35 AS.seek 0.594ml @02:32:30.93 delay 5 seconds @02:32:35.95 ATV.dial :ASV @02:32:36.36 delay 2 seconds @02:32:38.45 AS.seek 0.6ml @02:32:44.65 delay 4 seconds @02:32:48.67 ATV.dial ARV @02:32:49.22 delay 2 seconds @02:32:51.24 ARV.dial :carrier @02:32:51.26 delay 2 seconds @02:32:53.34 AS.seek 0.65ml @02:33:43.82 delay 4 seconds @02:33:47.84 ATV.dial PCR @02:33:48.45 ASV.dial :waste1 @02:33:48.46 ARV.dial :air1 @02:33:48.87 Positioning Slug by 0.324ml @02:33:49.52 AS.seek 0.326ml @02:39:15.79 delay 5 seconds @02:39:20.81 ATV.dial ARV @02:39:21.59 ASV.dial :PCRlock @02:39:21.98 delay 5 seconds @02:39:27.01 Running Bb Profile60C300 thermocycling @02:39:27.02 PCR.program Profile60C300 @02:39:27.16 Profile60C300 should complete in < 1:45:21 @02:39:27.17 PCR.run 2:16:41.25 @04:04:03.85 PCR.off @04:04:06.01 PCR for Bb completed -- closed /var/log/esp/Bb18sep1002h1500ml.pcr @04:04:06.08 Cleaning PCR @04:04:06.09 AFV.select :carrierSolution @04:04:06.21 delay 2 seconds @04:04:08.24 AS.reconfigure ASconfig @04:04:08.29 AS.seek 0.5ml @04:04:18.59 delay 1 second @04:04:19.60 AFV.select :holdingCoil @04:04:19.71 ASV.dial :waste1 @04:04:20.09 ATV.dial :air3 @04:04:21.36 AS.reconfigure ASslow1 @04:04:21.42 AS.seek 0.51ml @04:04:23.69 delay 2 seconds @04:04:25.71 ATV.dial :bleach @04:04:26.33 AS.seek 0.81ml @04:05:26.93 delay 3 seconds @04:05:29.95 ATV.dial :air3 @04:05:30.49 delay 3 seconds @04:05:33.58 AS.seek 0.835ml @04:05:38.88 delay 3 seconds @04:05:41.90 ATV.dial :bleach @04:05:42.53 AS.seek 0.82ml @04:05:45.84 delay 3 seconds @04:05:48.86 ARV.dial :waste @04:05:49.49 delay 3 seconds @04:05:52.51 ATV.dial :ARV @04:05:53.41 AS.reconfigure ASconfig @04:05:53.46 AS.seek 0.47ml @04:06:13.87 AFV.select :carrierSolution @04:06:13.99 delay 2 seconds @04:06:16.02 AS.seek 1ml @04:06:46.82 delay 1 second @04:06:47.83 AFV.select :holdingCoil @04:06:47.94 delay 1 second @04:06:48.97 AS.reconfigure ASfast @04:06:49.02 AS.seek 0ml @04:07:16.39 AFV.select :carrierSolution @04:07:16.51 delay 2 seconds @04:07:18.54 AS.reconfigure ASconfig @04:07:18.59 AS.seek 1ml @04:08:16.38 delay 1 second @04:08:17.39 AFV.select :holdingCoil @04:08:17.50 delay 1 second @04:08:18.53 AS.reconfigure ASfast @04:08:18.58 AS.seek 0ml @04:08:45.90 AFV.select :carrierSolution @04:08:46.02 delay 2 seconds @04:08:48.05 AS.reconfigure ASconfig @04:08:48.10 AS.seek 1ml @04:09:45.90 delay 1 second @04:09:46.91 AFV.select :holdingCoil @04:09:47.02 delay 1 second @04:09:48.04 AS.reconfigure ASfast @04:09:48.09 AS.seek 0ml @04:10:15.42 ARV.dial between carrier and air1 @04:10:15.93 ATV.dial :air3 @04:10:17.22 AS.reconfigure ASslow1 @04:10:17.27 AS.seek 0.01ml @04:10:19.73 delay 2 seconds @04:10:21.74 ATV.dial :bleach @04:10:22.36 AS.seek 0.81ml @04:13:03.51 delay 3 seconds @04:13:06.53 ATV.dial :air3 @04:13:07.07 delay 3 seconds @04:13:10.16 AS.seek 0.835ml @04:13:15.43 delay 3 seconds @04:13:18.45 ATV.dial :bleach @04:13:19.08 AS.seek 0.82ml @04:13:22.40 delay 3 seconds @04:13:25.42 ATV.dial PCR @04:13:25.82 AS.reconfigure ASconfig @04:13:25.87 AS.seek :empty @04:14:13.34 PCR.program :bleachProfile @04:14:13.41 PCR program should complete in < 2:45 @04:14:13.42 PCR.run 10:00 @04:17:06.14 PCR.off @04:17:06.16 AFV.select :carrierSolution @04:17:06.28 delay 2 seconds @04:17:08.31 AS.seek 1ml @04:18:06.13 delay 1 second @04:18:07.14 AFV.select :holdingCoil @04:18:07.25 delay 1 second @04:18:08.28 AS.reconfigure ASfast @04:18:08.33 AS.seek 0ml @04:18:35.70 delay 3 seconds @04:18:38.72 AFV.select :carrierSolution @04:18:38.83 delay 2 seconds @04:18:40.86 AS.reconfigure ASconfig @04:18:40.92 AS.seek 1ml @04:19:38.74 delay 1 second @04:19:39.75 AFV.select :holdingCoil @04:19:39.86 delay 1 second @04:19:40.89 AS.reconfigure ASfast @04:19:40.94 AS.seek 0ml @04:20:08.30 delay 3 seconds @04:20:11.32 AFV.select :carrierSolution @04:20:11.43 delay 2 seconds @04:20:13.46 AS.reconfigure ASconfig @04:20:13.53 AS.seek 1ml @04:21:11.32 delay 1 second @04:21:12.33 AFV.select :holdingCoil @04:21:12.44 delay 1 second @04:21:13.47 AS.reconfigure ASfast @04:21:13.52 AS.seek 0ml @04:21:40.88 delay 3 seconds @04:21:43.90 AFV.select :carrierSolution @04:21:44.01 delay 2 seconds @04:21:46.04 AS.reconfigure ASconfig @04:21:46.10 AS.seek 1ml @04:22:43.84 delay 1 second @04:22:44.86 AFV.select :holdingCoil @04:22:44.97 delay 1 second @04:22:46.00 AS.reconfigure ASfast @04:22:46.05 AS.seek 0ml @04:23:13.41 delay 3 seconds @04:23:16.43 AFV.select :carrierSolution @04:23:16.54 delay 2 seconds @04:23:18.57 AS.reconfigure ASconfig @04:23:18.63 AS.seek 1ml @04:24:16.41 delay 1 second @04:24:17.42 AFV.select :holdingCoil @04:24:17.53 delay 1 second @04:24:18.56 AS.reconfigure ASnorm @04:24:18.61 AS.seek 0ml @04:25:09.32 Setting up PCR for IPc -- writing /var/log/esp/IPc18sep1004h1500ml.pcr @04:25:09.34 Building PCR Slug from ASV with MM1 mix, PP1 primer @04:25:09.35 Priming with carrier @04:25:09.36 ATV.dial ARV @04:25:09.96 ARV.dial :carrier @04:25:10.33 AS.seek 0.5ml @04:25:35.92 AFV.select :carrierSolution @04:25:36.04 delay 2 seconds @04:25:38.07 AS.reconfigure ASconfig @04:25:38.13 AS.seek 1ml @04:26:07.15 delay 1 second @04:26:08.16 AFV.select :holdingCoil @04:26:08.27 delay 1 second @04:26:09.29 ATV.dial :waste @04:26:10.94 AS.reconfigure ASnorm @04:26:10.99 AS.seek :empty @04:27:01.72 AFV.select :carrierSolution @04:27:01.84 delay 2 seconds @04:27:03.87 AS.reconfigure ASconfig @04:27:03.92 AS.seek 0.5ml @04:27:33.04 delay 1 second @04:27:34.05 AFV.select :holdingCoil @04:27:34.16 Building Slug @04:27:34.17 ATV.dial :ARV @04:27:35.79 delay 2 seconds @04:27:37.81 ARV.dial :air1 @04:27:38.23 delay 2 seconds @04:27:40.32 AS.reconfigure ASslow2 @04:27:40.37 AS.seek 0.506ml @04:27:46.93 delay 2 seconds @04:27:48.95 ARV.dial :MM1 @04:27:49.55 delay 2 seconds @04:27:51.58 Adding MM1 @04:27:51.65 AS.seek 0.524ml @04:28:09.96 delay 25 seconds @04:28:34.98 ARV.dial :PP1 @04:28:35.38 Adding PP1 @04:28:35.39 delay 2 seconds @04:28:37.48 AS.seek 0.53ml @04:28:43.94 delay 10 seconds @04:28:53.96 ARV.dial :air1 @04:28:54.35 adding ARV air @04:28:54.36 delay 2 seconds @04:28:56.45 AS.seek 0.536ml @04:29:02.80 delay 5 seconds @04:29:07.82 ARV.dial :carrier @04:29:08.24 delay 7 seconds @04:29:15.33 AS.seek 0.594ml @04:30:13.93 delay 5 seconds @04:30:18.95 ATV.dial :ASV @04:30:19.35 delay 2 seconds @04:30:21.44 AS.seek 0.6ml @04:30:27.63 delay 4 seconds @04:30:31.65 ATV.dial ARV @04:30:32.19 delay 2 seconds @04:30:34.21 ARV.dial :carrier @04:30:34.23 delay 2 seconds @04:30:36.32 AS.seek 0.65ml @04:31:27.03 delay 4 seconds @04:31:31.05 ATV.dial PCR @04:31:31.68 ASV.dial :waste1 @04:31:31.69 ARV.dial :air1 @04:31:32.09 Positioning Slug by 0.324ml @04:31:32.18 AS.seek 0.326ml @04:36:58.60 delay 5 seconds @04:37:03.62 ATV.dial ARV @04:37:05.07 ASV.dial :PCRlock @04:37:05.46 delay 5 seconds @04:37:10.49 Running IPc ProfileIPc thermocycling @04:37:10.50 PCR.program ProfileIPc @04:37:10.65 ProfileIPc should complete in < 1:50:49 @04:37:10.66 PCR.run 2:23:31.25 @06:04:01.92 PCR.off @06:04:04.74 PCR for IPc completed -- closed /var/log/esp/IPc18sep1004h1500ml.pcr @06:04:04.75 Full MFB Decontamination @06:04:04.77 Decon extraction column and decon AIV @06:04:04.79 SPE.hold 2:46:40,55C @06:04:04.83 ASV.dial :SPEdelivery @06:04:05.50 delay 20 seconds @06:04:25.53 AFV.select :carrierSolution @06:04:25.64 delay 2 seconds @06:04:27.67 AS.reconfigure ASconfig @06:04:27.72 AS.seek 0.5ml @06:04:38.06 delay 1 second @06:04:39.07 AFV.select :holdingCoil @06:04:39.18 ATV.dial :air1 @06:04:41.32 AS.reconfigure ASslow1 @06:04:41.37 AS.seek 0.52ml @06:04:45.69 ATV.dial :ASV @06:04:47.34 ASV.dial :SPErecovery @06:04:48.04 AS.seek 0.62ml @06:05:08.40 ATV.dial :waste @06:05:10.47 AS.reconfigure ASnorm @06:05:10.52 AS.seek :empty @06:05:42.15 AFV.select :holdingCoil @06:05:42.17 ATV.dial :waste @06:05:42.19 AS.reconfigure ASconfig @06:05:42.24 AS.seek 0ml @06:05:42.52 AFV.select :carrierSolution @06:05:42.64 delay 2 seconds @06:05:44.67 AS.seek 0.5ml @06:06:13.69 delay 1 second @06:06:14.70 AFV.select :holdingCoil @06:06:14.81 ATV.dial :air3 @06:06:15.54 AS.reconfigure ASslow1 @06:06:15.59 AS.seek 0.52ml @06:06:19.88 ATV.dial :air3 @06:06:19.98 AS.seek 0.53ml @06:06:22.28 delay 2 seconds @06:06:24.29 ATV.dial :bleach @06:06:24.77 AS.seek 0.71ml @06:07:01.24 delay 3 seconds @06:07:04.26 ATV.dial :air3 @06:07:04.85 delay 3 seconds @06:07:07.94 AS.seek 0.735ml @06:07:13.17 delay 3 seconds @06:07:16.20 ATV.dial :bleach @06:07:16.84 AS.seek 0.72ml @06:07:20.14 delay 3 seconds @06:07:23.17 ATV.dial ASV @06:07:24.25 ASV.dial :SPErecovery @06:07:24.34 AS.seek 0.365ml @06:08:35.98 ATV.dial :waste @06:08:37.75 AS.reconfigure ASnorm @06:08:37.80 AS.seek :empty @06:08:56.60 ATV.dial :air3 @06:08:57.29 AS.reconfigure ASslow1 @06:08:57.34 AS.seek 0.009ml @06:08:59.69 delay 2 seconds @06:09:01.71 ATV.dial :bleach @06:09:02.20 AS.seek 0.579ml @06:10:57.06 delay 3 seconds @06:11:00.08 ATV.dial :air3 @06:11:00.64 delay 3 seconds @06:11:04.27 AS.seek 0.604ml @06:11:09.56 delay 3 seconds @06:11:12.58 ATV.dial :bleach @06:11:13.21 AS.seek 0.589ml @06:11:16.52 delay 3 seconds @06:11:19.54 ATV.dial :ASV @06:11:20.58 ASV.dial :wasteR @06:11:20.98 AS.seek 0ml @06:13:19.74 delay 1:00 @06:14:19.76 ATV.dial ASV @06:14:19.78 AFV.select :carrierSolution @06:14:19.89 delay 2 seconds @06:14:21.92 AS.reconfigure ASconfig @06:14:21.97 AS.seek 1ml @06:15:19.74 delay 1 second @06:15:20.75 AFV.select :holdingCoil @06:15:20.86 delay 1 second @06:15:21.88 ASV.dial :wasteR @06:15:21.90 AS.reconfigure ASslow1 @06:15:21.95 AS.seek 0ml @06:18:43.36 delay 10 seconds @06:18:53.38 ATV.dial :air3 @06:18:54.90 AS.seek 0.01ml @06:18:57.22 delay 2 seconds @06:18:59.23 ATV.dial :bleach @06:18:59.87 AS.seek 0.58ml @06:20:54.77 delay 3 seconds @06:20:57.79 ATV.dial :air3 @06:20:58.36 delay 3 seconds @06:21:01.45 AS.seek 0.605ml @06:21:06.84 delay 3 seconds @06:21:09.86 ATV.dial :bleach @06:21:10.49 AS.seek 0.59ml @06:21:13.77 delay 3 seconds @06:21:16.79 ATV.dial ASV @06:21:17.83 ASV.dial :SPEdelivery @06:21:18.21 AS.seek 0ml @06:23:17.13 delay 1:00 @06:24:17.15 AFV.select :carrierSolution @06:24:17.26 delay 2 seconds @06:24:19.29 AS.reconfigure ASconfig @06:24:19.34 AS.seek 1ml @06:25:17.16 delay 1 second @06:25:18.18 AFV.select :holdingCoil @06:25:18.29 delay 1 second @06:25:19.31 ATV.dial ASV @06:25:19.33 ASV.dial :SPEdelivery @06:25:19.35 AS.reconfigure ASslow1 @06:25:19.39 AS.seek 0ml @06:28:40.80 delay 10 seconds @06:28:50.82 ATV.dial :air2 @06:28:51.64 AS.reconfigure ASslow0 @06:28:51.70 AS.seek 0.8ml @06:30:12.50 delay 3 seconds @06:30:15.52 ATV.dial :ASV @06:30:16.50 ASV.dial :SPEdelivery @06:30:16.51 AS.reconfigure ASslow1 @06:30:16.56 AS.seek 0.03ml @06:32:51.72 delay 20 seconds @06:33:11.75 ATV.dial :waste,via: :air3 @06:33:13.48 AS.reconfigure ASconfig @06:33:13.53 AS.seek :empty @06:33:15.62 SPE.coast @06:33:15.71 Removing Bleach from Mixing Coil @06:33:15.72 AFV.select :carrierSolution @06:33:15.83 delay 2 seconds @06:33:17.86 AS.seek 0.5ml @06:33:46.97 delay 1 second @06:33:47.98 AFV.select :holdingCoil @06:33:48.09 ATV.dial :ASV @06:33:49.78 ASV.dial :SPErecovery @06:33:50.45 AS.reconfigure ASslow1 @06:33:50.50 AS.seek 0.73ml @06:34:37.04 ASV.dial :wasteD @06:34:37.63 AS.reconfigure ASnorm @06:34:37.69 AS.seek 0ml @06:35:14.82 AFV.select :carrierSolution @06:35:14.94 delay 2 seconds @06:35:16.97 AS.reconfigure ASconfig @06:35:17.02 AS.seek 0.5ml @06:35:46.08 delay 1 second @06:35:47.09 AFV.select :holdingCoil @06:35:47.20 ATV.dial ASV @06:35:47.21 ASV.dial :SPErecovery @06:35:47.87 AS.reconfigure ASslow0 @06:35:47.92 AS.seek 0.3ml @06:36:08.38 ATV.dial :air2 @06:36:09.42 AS.reconfigure ASslow1 @06:36:09.48 AS.seek 0.32ml @06:36:13.79 ATV.dial ASV @06:36:14.93 AS.reconfigure ASslow0 @06:36:14.98 AS.seek 0.57ml @06:36:40.46 ASV.dial :wasteD @06:36:41.14 AS.reconfigure ASnorm @06:36:41.77 AS.seek 0ml @06:37:10.86 AFV.select :carrierSolution @06:37:10.98 delay 2 seconds @06:37:13.01 AS.reconfigure ASconfig @06:37:13.07 AS.seek 0.5ml @06:37:42.14 delay 1 second @06:37:43.16 AFV.select :holdingCoil @06:37:43.27 ATV.dial ASV @06:37:43.28 ASV.dial :SPErecovery @06:37:43.93 AS.reconfigure ASslow0 @06:37:43.98 AS.seek 0.3ml @06:38:04.37 ATV.dial :air2 @06:38:05.40 AS.reconfigure ASslow1 @06:38:05.45 AS.seek 0.32ml @06:38:09.82 ATV.dial ASV @06:38:10.87 AS.reconfigure ASslow0 @06:38:10.92 AS.seek 0.57ml @06:38:36.38 ASV.dial :wasteD @06:38:36.97 AS.reconfigure ASnorm @06:38:37.03 AS.seek 0ml @06:39:06.11 ATV.dial :air2 @06:39:07.08 AS.reconfigure ASslow0 @06:39:07.14 AS.seek 0.3ml @06:39:37.69 ATV.dial :ASV @06:39:38.66 ASV.dial :wasteD @06:39:38.68 AS.reconfigure ASconfig @06:39:38.73 AS.seek 0.05ml @06:39:53.41 ATV.dial :waste @06:39:55.26 AS.seek 0ml @06:39:58.45 ASV.dial :SPEdelivery @06:39:59.42 deconLysateINT @06:39:59.43 ATV.dial :air3 @06:40:00.10 AS.reconfigure ASslow1 @06:40:00.15 AS.seek 0.01ml @06:40:02.58 delay 2 seconds @06:40:04.59 ATV.dial :bleach @06:40:05.07 AS.seek 0.96ml @06:43:16.40 delay 3 seconds @06:43:19.42 ATV.dial :air3 @06:43:19.98 delay 3 seconds @06:43:23.06 AS.seek 0.985ml @06:43:28.47 delay 3 seconds @06:43:31.49 ATV.dial :bleach @06:43:32.12 AS.seek 0.97ml @06:43:35.41 delay 3 seconds @06:43:38.44 ATV.dial AIV @06:43:39.67 AIV.dial :coilToMFB @06:43:39.68 AWV.select :waste @06:43:39.69 AFV.select :carrierSolution @06:43:39.81 delay 2 seconds @06:43:41.84 AS.reconfigure ASconfig @06:43:41.89 AS.seek 1ml @06:43:43.92 delay 1 second @06:43:44.93 AFV.select :holdingCoil @06:43:45.04 delay 1 second @06:43:46.07 AS.reconfigure ASslow0 @06:43:46.11 AS.seek :empty @06:45:27.08 AFV.select :carrierSolution @06:45:27.20 delay 2 seconds @06:45:29.23 AS.reconfigure ASconfig @06:45:29.28 AS.seek 1ml @06:46:27.09 delay 1 second @06:46:28.10 AFV.select :holdingCoil @06:46:28.21 delay 1 second @06:46:29.24 AS.reconfigure ASslow0 @06:46:29.28 AS.seek :empty @06:48:10.25 AFV.select :carrierSolution @06:48:10.37 delay 2 seconds @06:48:12.40 AS.reconfigure ASconfig @06:48:12.45 AS.seek 1ml @06:49:10.26 delay 1 second @06:49:11.27 AFV.select :holdingCoil @06:49:11.38 delay 1 second @06:49:12.41 AS.reconfigure ASslow0 @06:49:12.46 AS.seek :empty @06:50:53.43 ATV.dial :air2 @06:50:54.12 AS.reconfigure ASslow1 @06:50:54.17 AS.seek 0.009ml @06:50:56.53 AS.reconfigure ASslow0 @06:50:56.58 AS.seek 1ml @06:52:36.52 ATV.dial AIV @06:52:37.31 AS.seek 0.02ml @06:54:16.21 ATV.dial :waste @06:54:17.87 AS.seek 0ml @06:54:20.15 AWV.select :air @06:54:20.32 Cleaning PCR @06:54:20.34 AFV.select :carrierSolution @06:54:20.44 delay 2 seconds @06:54:22.47 AS.reconfigure ASconfig @06:54:22.52 AS.seek 0.5ml @06:54:51.59 delay 1 second @06:54:52.60 AFV.select :holdingCoil @06:54:52.71 ASV.dial :waste1 @06:54:53.64 ATV.dial :air3 @06:54:54.32 AS.reconfigure ASslow1 @06:54:54.37 AS.seek 0.51ml @06:54:56.79 delay 2 seconds @06:54:58.81 ATV.dial :bleach @06:54:59.29 AS.seek 0.81ml @06:56:00.02 delay 3 seconds @06:56:03.04 ATV.dial :air3 @06:56:03.61 delay 3 seconds @06:56:06.70 AS.seek 0.835ml @06:56:12.02 delay 3 seconds @06:56:15.04 ATV.dial :bleach @06:56:15.66 AS.seek 0.82ml @06:56:18.97 delay 3 seconds @06:56:22.00 ARV.dial :waste @06:56:22.62 delay 3 seconds @06:56:25.64 ATV.dial :ARV @06:56:26.54 AS.reconfigure ASconfig @06:56:26.59 AS.seek 0.47ml @06:56:47.00 AFV.select :carrierSolution @06:56:47.12 delay 2 seconds @06:56:49.15 AS.seek 1ml @06:57:19.93 delay 1 second @06:57:20.95 AFV.select :holdingCoil @06:57:21.06 delay 1 second @06:57:22.09 AS.reconfigure ASfast @06:57:22.14 AS.seek 0ml @06:57:49.49 AFV.select :carrierSolution @06:57:49.61 delay 2 seconds @06:57:51.64 AS.reconfigure ASconfig @06:57:51.69 AS.seek 1ml @06:58:49.44 delay 1 second @06:58:50.45 AFV.select :holdingCoil @06:58:50.56 delay 1 second @06:58:51.59 AS.reconfigure ASfast @06:58:51.64 AS.seek 0ml @06:59:18.97 AFV.select :carrierSolution @06:59:19.09 delay 2 seconds @06:59:21.12 AS.reconfigure ASconfig @06:59:21.17 AS.seek 1ml @07:00:18.94 delay 1 second @07:00:19.95 AFV.select :holdingCoil @07:00:20.06 delay 1 second @07:00:21.09 AS.reconfigure ASfast @07:00:21.14 AS.seek 0ml @07:00:48.49 ARV.dial between carrier and air1 @07:00:49.00 ATV.dial :air3 @07:00:50.29 AS.reconfigure ASslow1 @07:00:50.34 AS.seek 0.01ml @07:00:52.72 delay 2 seconds @07:00:54.73 ATV.dial :bleach @07:00:55.37 AS.seek 0.81ml @07:03:36.65 delay 3 seconds @07:03:39.67 ATV.dial :air3 @07:03:40.23 delay 3 seconds @07:03:43.31 AS.seek 0.835ml @07:03:48.63 delay 3 seconds @07:03:51.65 ATV.dial :bleach @07:03:52.29 AS.seek 0.82ml @07:03:55.65 delay 3 seconds @07:03:58.67 ATV.dial PCR @07:03:59.08 AS.reconfigure ASconfig @07:03:59.13 AS.seek :empty @07:04:46.60 PCR.program :bleachProfile @07:04:46.67 PCR program should complete in < 2:45 @07:04:46.68 PCR.run 10:00 @07:07:45.46 PCR.off @07:07:45.49 AFV.select :carrierSolution @07:07:45.60 delay 2 seconds @07:07:47.63 AS.seek 1ml @07:08:45.42 delay 1 second @07:08:46.43 AFV.select :holdingCoil @07:08:46.54 delay 1 second @07:08:47.57 AS.reconfigure ASfast @07:08:47.61 AS.seek 0ml @07:09:15.08 delay 3 seconds @07:09:18.10 AFV.select :carrierSolution @07:09:18.21 delay 2 seconds @07:09:20.24 AS.reconfigure ASconfig @07:09:20.29 AS.seek 1ml @07:10:18.04 delay 1 second @07:10:19.05 AFV.select :holdingCoil @07:10:19.16 delay 1 second @07:10:20.19 AS.reconfigure ASfast @07:10:20.24 AS.seek 0ml @07:10:47.65 delay 3 seconds @07:10:50.67 AFV.select :carrierSolution @07:10:50.78 delay 2 seconds @07:10:53.31 AS.reconfigure ASconfig @07:10:53.43 AS.seek 1ml @07:11:51.25 delay 1 second @07:11:52.26 AFV.select :holdingCoil @07:11:52.37 delay 1 second @07:11:53.40 AS.reconfigure ASfast @07:11:53.45 AS.seek 0ml @07:12:20.81 delay 3 seconds @07:12:23.83 AFV.select :carrierSolution @07:12:23.94 delay 2 seconds @07:12:25.97 AS.reconfigure ASconfig @07:12:26.02 AS.seek 1ml @07:13:23.83 delay 1 second @07:13:24.85 AFV.select :holdingCoil @07:13:24.96 delay 1 second @07:13:25.99 AS.reconfigure ASfast @07:13:26.04 AS.seek 0ml @07:13:53.40 delay 3 seconds @07:13:56.42 AFV.select :carrierSolution @07:13:56.53 delay 2 seconds @07:13:58.56 AS.reconfigure ASconfig @07:13:58.62 AS.seek 1ml @07:14:56.39 delay 1 second @07:14:57.40 AFV.select :holdingCoil @07:14:57.51 delay 1 second @07:14:58.55 AS.reconfigure ASnorm @07:14:58.60 AS.seek 0ml @07:15:49.30 AFV.select :carrierSolution @07:15:49.41 delay 2 seconds @07:15:51.44 AS.reconfigure ASconfig @07:15:51.49 AS.seek 1ml @07:16:49.23 delay 1 second @07:16:50.24 AFV.select :holdingCoil @07:16:50.35 delay 1 second @07:16:51.37 ATV.dial :waste @07:16:52.59 AS.reconfigure ASnorm @07:16:52.64 AS.seek 0.5ml @07:17:18.20 ATV.dial :waste2 @07:17:19.47 AS.seek 0ml @07:17:45.04
Asleep after completing 18tun1 assays @07:17:45.06 Mission runtime was 13:31:25.219224 @07:17:45.08 GoodNews.email "{1 => 2, 2 => 0}\nCan@07:16:20, 23.5C, 52% humidity, 14.6psia, 12.060V, 0.487A, 0.481A avg, 5.87W",Subject: "Asleep after completing 18tun1 assays" @14:25:54.85CEST12-Sep-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @14:25:56.95 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @14:25:57.24 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @14:26:03.08 Polling Can every 10:00 @14:26:04.92 SPE.coast @14:26:04.99 SPE.configure SPEconfig @14:26:05.09 AS.reconfigure ASconfig @14:26:05.13 AS.hold @14:26:05.19 AS.configure ASconfig @14:26:05.31 SS.reconfigure SSconfig @14:26:05.35 SS.hold @14:26:05.41 SS.configure SSconfig @14:26:05.51 CC.reconfigure CCconfig @14:26:05.57 CC.hold @14:26:05.65 CC.configure CCconfig @14:26:05.75 CS.reconfigure CSconfig @14:26:05.79 CS.hold @14:26:05.87 CS.configure CSconfig @14:26:06.36 PC.reconfigure PCconfig @14:26:06.41 PC.hold @14:26:06.46 PC.configure PCconfig @14:26:06.57 PS.configure PSconfig @14:26:06.70 Forearm.reconfigure LooseForearm @14:26:06.75 Forearm.hold @14:26:06.81 Forearm.configure LooseForearm @14:26:06.91 Elbow.reconfigure LooseElbow @14:26:06.95 Elbow.hold @14:26:07.01 Elbow.configure LooseElbow @14:26:07.13 SC.reconfigure SCloose @14:26:07.18 SC.hold @14:26:07.24 SC.configure SCloose @14:26:07.34 SE.reconfigure SEconfig @14:26:07.38 SE.hold @14:26:07.44 SE.configure SEconfig @14:26:07.49 CH.coast @14:26:07.54 CH.configure CHconfig @14:26:07.58 PH.coast @14:26:07.63 PH.configure PHconfig @14:26:07.67 PCR.off @14:26:07.71 PCR.pidtemp= 50,0.1,0,300,25,25 @14:26:07.74 PCR.caltemp= 2.7073,0.0373,0 @14:26:07.77 All dwarf controllers running firmware version 3.75 @14:26:07.91 Loading /home/esp/esp2/mission/18tun1.rb mission @14:26:07.98 Preparing to sleep until +0:00:15 @14:26:08.00 GoodNews.email "Starting 18tun1 w/pucks in tube 2\n{}\nCan@14:26:03, 22.3C, 34% humidity, 14.7psia, 11.937V, 0.909A, 0.903A avg, 10.86W",Subject: "18tun1 begun" @14:26:08.15 AS.coast @14:26:08.23
Asleep til +0:00:15 @14:26:08.31 Continuing at 14:26 @14:26:08.37 SS.coast @14:26:08.57 CC.coast @14:26:08.64 CS.coast @14:26:08.71 PC.coast @14:26:08.81 Forearm.coast @14:26:08.91 Elbow.coast @14:26:08.98 SC.coast @14:26:09.04 SE.coast @14:26:09.26 Session Begun @14:26:09.59 Gate.power :core,:OFF @14:26:09.61 Gate.power :raw,:OFF @14:26:09.64 Shutdown @14:26:16.10 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:105\n{}\nCan@14:26:08, 22.3C, 34% humidity, 14.7psia, 11.928V, 0.922A, 0.922A avg, 11.00W",Subject: "Asleep until 14:26" @14:26:22.96
Powering core bus @14:26:28.00 Gate.power :raw,:ON @14:26:28.77 SPE.coast @14:26:28.88 SPE.configure SPEconfig @14:26:28.99 AS.configure ASconfig @14:26:29.11 SS.configure SSconfig @14:26:29.23 CC.configure CCconfig @14:26:29.35 CS.configure CSconfig @14:26:29.52 PC.configure PCconfig @14:26:29.64 PS.configure PSconfig @14:26:29.79 Forearm.configure LooseForearm @14:26:29.92 Elbow.configure LooseElbow @14:26:30.12 SC.configure SCloose @14:26:30.27 SE.configure SEconfig @14:26:30.34 CH.coast @14:26:30.42 CH.configure CHconfig @14:26:30.47 PH.coast @14:26:30.56 PH.configure PHconfig @14:26:30.62 PCR.off @14:26:30.67 PCR.pidtemp= 50,0.1,0,300,25,25 @14:26:30.72 PCR.caltemp= 2.7073,0.0373,0 @14:26:30.76 All dwarf controllers running firmware version 3.75 @14:26:30.90 Valve.reset @14:26:31.09 PCR.off @14:26:31.14 PCR.pidtemp= 50,0.1,0,300,25,25 @14:26:31.17 PCR.caltemp= 2.7073,0.0373,0 @14:26:31.39 Intake.select :close @14:26:31.41 Exhaust.select :close @14:26:31.43 CTV.dial :air @14:26:31.55 PSV.dial :air @14:26:31.58 CSV.dial :waste1 @14:26:31.80 SSV.dial :spit,avoiding: :exhaust @14:26:31.85 PS.reconfigure PSconfig @14:26:31.88
Forearm.reconfigure LooseForearm @14:26:32.46 PS.home.seek 0ml @14:26:32.49
Forearm.home.seek :retract @14:26:32.71 CS.reconfigure CSfast @14:26:32.74 SS.reconfigure SSconfig @14:26:32.87 CS.home.seek 0ml @14:26:32.90 SS.home.seek 0ml @14:26:33.30 Hand.close @14:26:33.34
Elbow.jog 1500 @14:26:33.92 Elbow.reconfigure LooseElbow @14:26:33.99 Elbow.home.seek :clear @14:26:38.64 SE.reconfigure SEconfig @14:26:38.72 SE.home.seek :down @14:26:39.42 SC.reconfigure SCsloppy @14:26:39.49 SC.home.seek :initial @14:26:41.82 PS.coast @14:26:43.70
Forearm.seek :retract @14:26:43.80 Elbow.seek :hammer @14:26:44.99 Forearm.reconfigure TightForearm @14:26:45.07 Forearm.seek 5 + 350 counts @14:26:46.19 Forearm.seek :retract @14:26:47.40 PC.reconfigure PCconfig @14:26:47.43
CC.reconfigure CCconfig @14:26:47.54 PC.home.seek :open @14:26:47.58
CC.home.seek :open @14:26:50.96 Phase [mbaPhase] @14:26:50.97 GoodNews.email "At 14:26:50 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:105\n{}\nCan@14:26:50, 22.3C, 34% humidity, 14.7psia, 11.972V, 0.758A, 0.758A avg, 9.08W",Subject: "Started 18tun1 phase mbaPhase" @14:26:51.01CEST12-Sep-18
==================> STARTED MBAsuperphase (phase #1) <=== @14:26:51.12 Starting mfb @14:26:51.14 pcrBAC sampling at most 1500ml, wcr at most 1200ml @14:26:51.23 Loading sh1 puck from tube 2 into Collection Clamp @14:26:51.37 Readying the MFB @14:26:51.40
Hand.open @14:26:51.43 Homing MFB Syringe @14:26:51.48 AFV.select :holdingCoil @14:26:51.67 ATV.dial :air2 @14:26:52.48
SC.reconfigure SCloose @14:26:52.67 Elbow.reconfigure TightElbow @14:26:52.74 Elbow.seek 2 @14:26:53.00 AS.reconfigure ASconfig @14:26:53.10 AS.home.seek :home @14:26:53.32
Forearm.seek 2 + 700 counts @14:26:54.32 SE.reconfigure SEpick @14:26:54.42 SE.seek beamBreak @14:26:55.45 SE.configure SEconfig @14:26:55.67 Elbow.seek 2 @14:26:55.93 Forearm.seek 2 + 300 counts @14:26:56.21 Forearm.reconfigure LooseForearm @14:26:56.28 Hand.close @14:26:57.13 SE.seek :down @14:26:57.37
Forearm.seek :retract @14:26:57.96 SE.coast @14:26:58.35
Elbow.seek CC @14:26:58.71 AFV.select :carrierSolution @14:26:58.83 delay 2 seconds @14:27:00.04
Elbow.reconfigure LooseElbow @14:27:00.17 Forearm.reconfigure TightForearm @14:27:00.25 Forearm.seek CC @14:27:00.87 AS.seek 1ml @14:27:01.55
CC.seek :closed @14:27:05.86 Collection Clamp onSpring/offSpring for 6/30 samples, on/off torque 2.58 @14:27:05.88 Hand.open @14:27:06.79 Forearm.reconfigure LooseForearm @14:27:06.87 Forearm.seek :retract @14:27:07.86 SC.coast @14:27:07.92 collectSample 1500ml w/bubble point of 55psi @14:27:07.94 Priming 7ml @14:27:07.96 Gate.power :sampler,:ON @14:27:07.99 Intake.select :close @14:27:08.01 Exhaust.select :close @14:27:08.04 SS.hold @14:27:08.31 Calibrating pressure and temperature sensors @14:27:08.34 CTV.dial :air @14:27:08.38 SSV.dial :spit,avoiding: :exhaust @14:27:08.46 SS.reconfigure SSfast @14:27:08.55 SS.seek 5ml @14:27:11.44 SSV.dial CBV @14:27:11.48
CBV.dial SSV @14:27:13.03 delay 3.8 seconds @14:27:16.97 SSV.dial :spit @14:27:18.94 SS.seek 0ml @14:27:21.95 SSV.dial CBV @14:27:23.47 CTV.dial :intake @14:27:24.36 Waiting for external sampler to finish priming... @14:27:24.37 delayUntil "14:32:07.99" @14:27:24.54 BACfans.email "Started mfb in tube 2 at 14:26:51CEST12-Sep-18\n{}\nCan@14:26:50, 22.3C, 34% humidity, 14.7psia, 11.972V, 0.758A, 0.758A avg, 9.08W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1200ml" @14:27:53.82 delay 1 second @14:27:54.85 AFV.select :holdingCoil @14:27:54.97 delay 1 second @14:27:56.00 ATV.dial :waste @14:27:57.22 AS.reconfigure ASnorm @14:27:57.30 AS.home.seek :empty @14:28:19.13 -> abort! "1" @14:28:19.15 CmdServer::Client::Error in bkha -- Aborting Mission: 1 @14:28:19.21
AS.stop @14:28:19.30 BadNews.email "1",Subject: "18tun1 ABORTED!" @14:28:27.43 Storage::Warning in MAIN -- Exiting with Collection Clamp holding Puck.from 2, :sh1 @14:28:27.48 Session Ended @14:42:42.32CEST12-Sep-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @14:42:44.81 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @14:42:45.17 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @14:42:51.63 Polling Can every 10:00 @14:42:53.50 SPE.coast @14:42:53.58 SPE.configure SPEconfig @14:42:53.72 AS.configure ASconfig @14:42:53.94 SS.reconfigure SSconfig @14:42:54.03 SS.hold @14:42:54.14 SS.configure SSconfig @14:42:54.34 CC.reconfigure CCconfig @14:42:54.42 CC.hold @14:42:54.54 CC.configure CCconfig @14:42:54.67 CS.reconfigure CSconfig @14:42:54.72 CS.hold @14:42:54.77 CS.configure CSconfig @14:42:54.87 PC.reconfigure PCconfig @14:42:54.92 PC.hold @14:42:55.39 PC.configure PCconfig @14:42:55.48 PS.configure PSconfig @14:42:55.60 Forearm.reconfigure LooseForearm @14:42:55.65 Forearm.hold @14:42:55.70 Forearm.configure LooseForearm @14:42:55.80 Elbow.reconfigure LooseElbow @14:42:55.85 Elbow.hold @14:42:55.90 Elbow.configure LooseElbow @14:42:56.02 SC.configure SCloose @14:42:56.12 SE.configure SEconfig @14:42:56.16 CH.coast @14:42:56.22 CH.configure CHconfig @14:42:56.25 PH.coast @14:42:56.32 PH.configure PHconfig @14:42:56.36 PCR.off @14:42:56.39 PCR.pidtemp= 50,0.1,0,300,25,25 @14:42:56.42 PCR.caltemp= 2.7073,0.0373,0 @14:42:56.45 All dwarf controllers running firmware version 3.75 @14:42:56.58 Cleanly exited at 14:42:17CEST12-Sep-18 @14:42:56.61 Loading /home/esp/esp2/mission/18tun1.rb mission @14:42:56.69 Preparing to sleep until +0:00:15 @14:42:56.71 GoodNews.email "Starting 18tun1 w/pucks in tube 2\n{}\nCan@14:42:51, 22.6C, 34% humidity, 14.7psia, 11.992V, 0.670A, 0.670A avg, 8.03W",Subject: "18tun1 begun" @14:42:56.80 SS.coast @14:42:56.83
Asleep til +0:00:15 @14:42:56.88 Continuing at 14:43 @14:42:56.89 CC.coast @14:42:56.98 CS.coast @14:42:57.04 PC.coast @14:42:57.10 Forearm.coast @14:42:57.17 Elbow.coast @14:42:57.66 Gate.power :core,:OFF @14:42:57.69 Gate.power :raw,:OFF @14:42:57.71 Gate.power :sampler,:OFF @14:42:57.73 Shutdown @14:42:57.85 Session Begun @14:43:06.64 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:106\n{}\nCan@14:42:56, 22.6C, 34% humidity, 14.7psia, 11.987V, 0.676A, 0.676A avg, 8.11W",Subject: "Asleep until 14:43" @14:43:06.70 -> showlog 100 @14:43:11.65
Powering core bus @14:43:16.42 Gate.power :raw,:ON @14:43:17.23 SPE.coast @14:43:17.32 SPE.configure SPEconfig @14:43:17.47 AS.configure ASconfig @14:43:17.63 SS.configure SSconfig @14:43:17.77 CC.configure CCconfig @14:43:17.91 CS.configure CSconfig @14:43:18.04 PC.configure PCconfig @14:43:18.17 PS.configure PSconfig @14:43:18.42 Forearm.configure LooseForearm @14:43:18.55 Elbow.configure LooseElbow @14:43:18.73 SC.configure SCloose @14:43:18.87 SE.configure SEconfig @14:43:18.93 CH.coast @14:43:19.02 CH.configure CHconfig @14:43:19.09 PH.coast @14:43:19.18 PH.configure PHconfig @14:43:19.24 PCR.off @14:43:19.28 PCR.pidtemp= 50,0.1,0,300,25,25 @14:43:19.33 PCR.caltemp= 2.7073,0.0373,0 @14:43:19.38 All dwarf controllers running firmware version 3.75 @14:43:19.52 Valve.reset @14:43:19.72 PCR.off @14:43:19.77 PCR.pidtemp= 50,0.1,0,300,25,25 @14:43:19.82 PCR.caltemp= 2.7073,0.0373,0 @14:43:20.08 Intake.select :close @14:43:20.10 Exhaust.select :close @14:43:20.14 CTV.dial :air @14:43:20.25 PSV.dial :air @14:43:20.42
Forearm.reconfigure LooseForearm @14:43:20.49 Forearm.home.seek :retract @14:43:20.58 PS.reconfigure PSconfig @14:43:20.65 PS.home.seek 0ml @14:43:21.01 CSV.dial :waste1 @14:43:21.26 Hand.close @14:43:21.30
Elbow.jog 1500 @14:43:21.59 SSV.dial :spit,avoiding: :exhaust @14:43:22.15
Elbow.reconfigure LooseElbow @14:43:22.32 Elbow.home.seek :clear @14:43:23.77 CS.reconfigure CSfast @14:43:23.81 SS.reconfigure SSconfig @14:43:23.91 CS.home.seek 0ml @14:43:24.04 SS.home.seek 0ml @14:43:26.87
SE.reconfigure SEconfig @14:43:26.94 SE.home.seek :down @14:43:27.61 SC.reconfigure SCsloppy @14:43:27.68 SC.home.seek :initial @14:43:29.83 PS.coast @14:43:31.93
Forearm.seek :retract @14:43:32.04 Elbow.seek :hammer @14:43:33.21 Forearm.reconfigure TightForearm @14:43:33.28 Forearm.seek 5 + 350 counts @14:43:34.35 Forearm.seek :retract @14:43:35.67 PC.reconfigure PCconfig @14:43:35.70
CC.reconfigure CCconfig @14:43:35.82 PC.home.seek :open @14:43:35.86
CC.home.seek :open @14:43:39.24 Phase [mbaPhase] @14:43:39.26 GoodNews.email "At 14:43:39 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:106\n{}\nCan@14:43:39, 22.6C, 34% humidity, 14.7psia, 11.982V, 0.701A, 0.701A avg, 8.40W",Subject: "Started 18tun1 phase mbaPhase" @14:43:39.30CEST12-Sep-18
==================> STARTED MBAsuperphase (phase #1) <=== @14:43:39.34 Starting initialPurge @14:43:39.36 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:43:39.47 Hand.open @14:43:40.44 Elbow.reconfigure TightElbow @14:43:40.53 Elbow.seek FlushPuck::Garage @14:43:42.44 Elbow.reconfigure LooseElbow @14:43:42.57 Forearm.reconfigure LooseForearm @14:43:42.65 Forearm.seek :garage @14:43:43.70 Hand.close @14:43:44.60 Forearm.seek :retract @14:43:45.71 Elbow.reconfigure TightElbow @14:43:45.79 Elbow.seek CC @14:43:48.64 Elbow.reconfigure LooseElbow @14:43:48.78 Forearm.reconfigure TightForearm @14:43:48.85 Forearm.seek CC @14:43:50.18 CC.seek :closed @14:43:54.44 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.50 @14:43:54.54 Hand.open @14:43:55.36 Forearm.reconfigure LooseForearm @14:43:55.43 Forearm.seek :retract @14:43:56.43 Flushing the Collection Valve::Manifold @14:43:56.59 CSV.dial CSR @14:43:58.16 Pulling 5ml of CSR.flush @14:43:58.17 CSR.select :flush @14:43:58.32 CS.reconfigure CSconfig @14:43:58.39 CS.seek 5ml @14:44:12.53 delay 5 seconds @14:44:17.56 CSR.select :air @14:44:17.76 CS.reconfigure CSfast @14:44:17.83 CS.seek 7.5ml @14:44:25.25 CSV.dial :waste @14:44:26.77 CS.reconfigure CSconfig @14:44:26.84 CS.seek 0ml @14:44:47.03 Purging external Intake @14:44:47.19 CSV.dial :flush @14:44:47.72 CS.reconfigure CSfast @14:44:47.79 CS.seek 10ml @14:45:14.34 delay 5 seconds @14:45:19.37 CSV.dial CBV @14:45:19.41
CBV.dial CSV @14:45:20.68 CTV.dial :intake @14:45:21.56 Intake.select :open @14:45:21.70 CS.seek 0ml @14:45:48.93 Intake.select :close @14:45:49.06 CTV.dial :air @14:45:49.92 Flushing upper manifold @14:45:50.08 CSV.dial :flush @14:45:50.94 CS.seek 5ml @14:46:04.72 delay 5 seconds @14:46:09.75 CSV.dial :air @14:46:10.48 CS.seek 7ml @14:46:16.65 CSV.dial CBV @14:46:16.68
CBV.dial CSV @14:46:17.98 CTV.dial :air @14:46:18.01 CS.seek 0ml @14:46:36.93 delay 7 seconds @14:46:43.97 CS.seek 3ml @14:46:52.72 CSV.dial :waste @14:46:53.24 CS.seek 0ml @14:47:02.02 CSV.dial :flush @14:47:03.01 CS.seek 3ml @14:47:11.68 delay 5 seconds @14:47:16.71 CSV.dial :air @14:47:17.31 CS.seek 5ml @14:47:23.43 CSV.dial :air @14:47:23.46 CS.seek 0ml @14:47:37.12 delay 7 seconds @14:47:44.16 CS.seek 3ml @14:47:52.81 CSV.dial :waste @14:47:53.68 CS.seek 0ml @14:48:02.65 Garaging FlushPuck in Collection Clamp @14:48:02.76 Elbow.reconfigure TightElbow @14:48:02.83 Elbow.seek CC @14:48:03.08 Elbow.reconfigure LooseElbow @14:48:03.21 Forearm.seek CC @14:48:04.21 Hand.close @14:48:04.93 CC.seek :open @14:48:09.32 Forearm.seek :retract @14:48:10.34 Elbow.reconfigure TightElbow @14:48:10.42 Elbow.seek :garage @14:48:13.51 Elbow.reconfigure LooseElbow @14:48:13.64 Forearm.reconfigure TightForearm @14:48:13.71 Forearm.seek :garage @14:48:14.92 Hand.open @14:48:15.74 Forearm.reconfigure LooseForearm @14:48:15.81 Forearm.seek :retract @14:48:16.99 Starting mfb @14:48:17.01 pcrBAC sampling at most 1500ml, wcr at most 1200ml @14:48:17.06 BACfans.email "Started mfb in tube 2 at 14:48:17CEST12-Sep-18\n{}\nCan@14:43:39, 22.6C, 34% humidity, 14.7psia, 11.982V, 0.701A, 0.701A avg, 8.40W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1200ml" @14:48:17.13
Loading sh1 puck from tube 2 into Collection Clamp @14:48:17.32 Readying the MFB @14:48:17.40 Homing MFB Syringe @14:48:17.43 AFV.select :holdingCoil @14:48:17.69 ATV.dial :waste2 @14:48:17.87
SC.reconfigure SCloose @14:48:18.17 Elbow.reconfigure TightElbow @14:48:18.24 Elbow.seek 2 @14:48:19.24 AS.reconfigure ASconfig @14:48:19.31 AS.home.seek :home @14:48:20.43
Forearm.reconfigure TightForearm @14:48:20.51 Forearm.seek 2 + 700 counts @14:48:21.61 SE.reconfigure SEpick @14:48:21.73 SE.seek beamBreak @14:48:22.91 SE.configure SEconfig @14:48:23.13 Elbow.seek 2 @14:48:23.37 Forearm.seek 2 + 300 counts @14:48:23.66 Forearm.reconfigure LooseForearm @14:48:23.74 Hand.close @14:48:24.57 SE.seek :down @14:48:24.84
Forearm.seek :retract @14:48:25.50 SE.coast @14:48:25.84
Elbow.seek CC @14:48:27.46 Elbow.reconfigure LooseElbow @14:48:28.06 Forearm.reconfigure TightForearm @14:48:28.13 Forearm.seek CC @14:48:29.47 CC.seek :closed @14:48:33.75 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.51 @14:48:33.77 Hand.open @14:48:34.62 Forearm.reconfigure LooseForearm @14:48:34.69 Forearm.seek :retract @14:48:35.72 SC.coast @14:48:35.78 collectSample 1500ml w/bubble point of 55psi @14:48:35.79 Priming 7ml @14:48:35.82 Intake.select :close @14:48:35.84 Exhaust.select :close @14:48:35.88 SS.hold @14:48:36.16 Calibrating pressure and temperature sensors @14:48:36.18 CTV.dial :air @14:48:36.22 SSV.dial :spit,avoiding: :exhaust @14:48:36.26 SS.reconfigure SSfast @14:48:36.34 SS.seek 5ml @14:48:39.07 SSV.dial CBV @14:48:39.11
CBV.dial SSV @14:48:40.66 delay 3.8 seconds @14:48:44.60 SSV.dial :spit @14:48:46.11 SS.seek 0ml @14:48:48.74 SSV.dial CBV @14:48:49.04 AFV.select :carrierSolution @14:48:49.17 delay 2 seconds @14:48:50.24
CTV.dial :intake @14:48:51.11 SSV.dial CBV @14:48:51.14
CBV.dial SSV @14:48:51.18 Allowing 40s for 7.0ml of remaining 7.0ml @14:48:51.21 Intake.select :open @14:48:51.31 AS.seek 1ml @14:48:53.44
Intake @15.7psia, vacuum from 9.2 to 13.2psi @14:48:53.47 SS.reconfigure SSinhale.with maxSpeed: 150 @14:48:53.56 SS.seek 7ml @14:48:59.59 Stroke completed -- waiting for pressure to equalize ... @14:49:12.92 Intake.select :close @14:49:13.10 SSV.dial :exhaust @14:49:13.98 Exhaust.select :open @14:49:14.12 SS.reconfigure SSexhale @14:49:14.19 SS.seek 0ml @14:49:18.71 Exhaust.select :close @14:49:18.83 Sampling 1500.0ml @14:49:18.85 Intake.select :close @14:49:18.87 Exhaust.select :close @14:49:18.89 SS.reconfigure SSconfig @14:49:18.97 SS.hold @14:49:19.21 CTV.dial :intake @14:49:19.23 SSV.dial CBV @14:49:19.27
CBV.dial SSV @14:49:20.15 Allowing 130s for 25.0ml of remaining 1500.0ml @14:49:20.17 Intake.select :open @14:49:22.78 Intake @15.6psia, vacuum from 9.1 to 13.1psi @14:49:22.90 SS.reconfigure SSinhale.with maxSpeed: 150 @14:49:22.99 SS.seek 25ml @14:49:26.39 Vacuum too high -- waiting up to 40s @14:49:31.54 Intake @15.2psia, vacuum from 8.7 to 12.7psi @14:49:31.57 SS.reconfigure SSinhale.with maxSpeed: 135 @14:49:31.66 SS.seek 25ml @14:49:34.83 Vacuum too high -- waiting up to 40s @14:49:40.79 Intake @15.4psia, vacuum from 8.9 to 12.9psi @14:49:40.81 SS.reconfigure SSinhale.with maxSpeed: 122 @14:49:40.89 SS.seek 25ml @14:49:44.14 Vacuum too high -- waiting up to 40s @14:49:44.21 delay 1 second @14:49:45.23 AFV.select :holdingCoil @14:49:45.43 delay 1 second @14:49:46.46 ATV.dial :waste @14:49:48.04 AS.reconfigure ASnorm @14:49:48.11 AS.home.seek :empty @14:49:49.65
Intake @15.4psia, vacuum from 8.9 to 12.9psi @14:49:49.67 SS.reconfigure SSinhale.with maxSpeed: 110 @14:49:49.76 SS.seek 25ml @14:49:53.14 Vacuum too high -- waiting up to 40s @14:49:58.29 Intake @15.3psia, vacuum from 8.8 to 12.8psi @14:49:58.31 SS.reconfigure SSinhale.with maxSpeed: 99 @14:49:58.40 SS.seek 25ml @14:50:01.89 Vacuum too high -- waiting up to 40s @14:50:07.78 Intake @15.3psia, vacuum from 8.8 to 12.8psi @14:50:07.80 SS.reconfigure SSinhale.with maxSpeed: 89 @14:50:07.89 SS.seek 25ml @14:50:11.67 Vacuum too high -- waiting up to 40s @14:50:17.33 Intake @15.4psia, vacuum from 8.9 to 12.9psi @14:50:17.35 SS.reconfigure SSinhale.with maxSpeed: 80 @14:50:17.44 SS.seek 25ml @14:50:18.59 Stroke completed -- waiting for pressure to equalize ... @14:50:20.56 Intake.select :close @14:50:20.73 SSV.dial :exhaust @14:50:21.60 Exhaust.select :open @14:50:21.74 SS.reconfigure SSexhale @14:50:21.86 SS.seek 0ml @14:50:31.35 Exhaust.select :close @14:50:31.49 SSV.dial CBV @14:50:31.53
CBV.dial SSV @14:50:32.43 Allowing 130s for 25.0ml of remaining 1475.0ml @14:50:32.45 Intake.select :open @14:50:34.65 Intake @15.6psia, vacuum from 9.1 to 13.1psi @14:50:34.68 SS.reconfigure SSinhale.with maxSpeed: 80 @14:50:34.77 SS.seek 25ml @14:50:39.31 ATV.dial :waste @14:50:39.33 ARV.dial between carrier and air1 @14:50:39.45 ASV.dial :waste1 @14:50:39.75
Vacuum too high -- waiting up to 40s @14:50:44.62 Intake @15.3psia, vacuum from 8.8 to 12.8psi @14:50:44.63 SS.reconfigure SSinhale.with maxSpeed: 72 @14:50:44.73 SS.seek 25ml @14:50:49.36 Vacuum too high -- waiting up to 40s @14:50:54.49 Intake @15.4psia, vacuum from 8.9 to 12.9psi @14:50:54.51 SS.reconfigure SSinhale.with maxSpeed: 65 @14:50:54.61 SS.seek 25ml @14:50:59.82 Vacuum too high -- waiting up to 40s @14:51:04.71 SS.reconfigure SSinhale.with maxSpeed: 59 @14:51:04.79 SS.seek 25ml @14:51:11.95 Vacuum too high -- waiting up to 40s @14:51:17.09 Intake @15.4psia, vacuum from 8.9 to 12.9psi @14:51:17.12 SS.reconfigure SSinhale.with maxSpeed: 53 @14:51:17.21 SS.seek 25ml @14:51:26.29 Stroke completed -- waiting for pressure to equalize ... @14:51:33.17 Intake.select :close @14:51:33.36 SSV.dial :exhaust @14:51:34.24 Exhaust.select :open @14:51:34.38 SS.reconfigure SSexhale @14:51:34.45 SS.seek 0ml @14:51:43.71 Exhaust.select :close @14:51:43.84 SSV.dial CBV @14:51:43.87
CBV.dial SSV @14:51:44.74 Allowing 130s for 25.0ml of remaining 1450.0ml @14:51:44.76 Intake.select :open @14:51:46.98 Intake @15.5psia, vacuum from 9.0 to 13.0psi @14:51:47.00 SS.reconfigure SSinhale.with maxSpeed: 53 @14:51:47.09 SS.seek 25ml @14:51:57.36 Vacuum too high -- waiting up to 40s @14:52:02.04 Intake @15.4psia, vacuum from 8.9 to 12.9psi @14:52:02.06 SS.reconfigure SSinhale.with maxSpeed: 48 @14:52:02.15 SS.seek 25ml @14:52:14.80 Vacuum too high -- waiting up to 40s @14:52:19.44 Intake @15.3psia, vacuum from 8.8 to 12.8psi @14:52:19.47 SS.reconfigure SSinhale.with maxSpeed: 43 @14:52:19.56 SS.seek 25ml @14:52:36.36 Stroke completed -- waiting for pressure to equalize ... @14:52:41.47 Intake.select :close @14:52:41.65 SSV.dial :exhaust @14:52:42.52 Exhaust.select :open @14:52:42.67 SS.reconfigure SSexhale @14:52:42.74 SS.seek 0ml @14:52:52.37 Exhaust.select :close @14:52:52.51 SSV.dial CBV @14:52:52.54
CBV.dial SSV @14:52:53.43 Allowing 130s for 25.0ml of remaining 1425.0ml @14:52:53.45 Intake.select :open @14:52:55.66 Intake @15.6psia, vacuum from 9.1 to 13.1psi @14:52:55.68 SS.reconfigure SSinhale.with maxSpeed: 43 @14:52:55.78 SS.seek 25ml @14:53:21.23 Vacuum too high -- waiting up to 40s @14:53:25.37 Intake @15.3psia, vacuum from 8.8 to 12.8psi @14:53:25.39 SS.reconfigure SSinhale.with maxSpeed: 39 @14:53:25.48 SS.seek 25ml @14:53:43.05 Stroke completed -- waiting for pressure to equalize ... @14:53:47.52 Intake.select :close @14:53:47.71 SSV.dial :exhaust @14:53:48.60 Exhaust.select :open @14:53:48.74 SS.reconfigure SSexhale @14:53:48.82 SS.seek 0ml @14:53:58.07 Exhaust.select :close @14:53:58.22 SSV.dial CBV @14:53:58.25
CBV.dial SSV @14:53:59.13 Allowing 130s for 25.0ml of remaining 1400.0ml @14:53:59.15 Intake.select :open @14:54:01.36 Intake @15.7psia, vacuum from 9.2 to 13.2psi @14:54:01.38 SS.reconfigure SSinhale.with maxSpeed: 39 @14:54:01.47 SS.seek 25ml @14:54:47.52 Stroke completed -- waiting for pressure to equalize ... @14:54:51.99 Intake.select :close @14:54:52.16 SSV.dial :exhaust @14:54:53.04 Exhaust.select :open @14:54:53.18 SS.reconfigure SSexhale @14:54:53.25 SS.seek 0ml @14:55:02.79 Exhaust.select :close @14:55:02.93 SSV.dial CBV @14:55:02.96
CBV.dial SSV @14:55:03.84 Allowing 130s for 25.0ml of remaining 1375.0ml @14:55:03.86 Intake.select :open @14:55:06.07 Intake @15.5psia, vacuum from 9.0 to 13.0psi @14:55:06.10 SS.reconfigure SSinhale.with maxSpeed: 39 @14:55:06.20 SS.seek 25ml @14:55:51.63 Stroke completed -- waiting for pressure to equalize ... @14:55:55.98 Intake.select :close @14:55:56.16 SSV.dial :exhaust @14:55:57.04 Exhaust.select :open @14:55:57.18 SS.reconfigure SSexhale @14:55:57.25 SS.seek 0ml @14:56:06.55 Exhaust.select :close @14:56:06.68 SSV.dial CBV @14:56:06.71
CBV.dial SSV @14:56:07.65 Allowing 130s for 25.0ml of remaining 1350.0ml @14:56:07.67 Intake.select :open @14:56:09.88 Intake @15.5psia, vacuum from 9.0 to 13.0psi @14:56:09.90 SS.reconfigure SSinhale.with maxSpeed: 39 @14:56:10.00 SS.seek 25ml @14:56:55.54 Stroke completed -- waiting for pressure to equalize ... @14:57:00.02 Intake.select :close @14:57:00.21 SSV.dial :exhaust @14:57:01.08 Exhaust.select :open @14:57:01.22 SS.reconfigure SSexhale @14:57:01.29 SS.seek 0ml @14:57:10.52 Exhaust.select :close @14:57:10.67 SSV.dial CBV @14:57:10.70
CBV.dial SSV @14:57:11.58 Allowing 130s for 25.0ml of remaining 1325.0ml @14:57:11.60 Intake.select :open @14:57:13.80 Intake @15.6psia, vacuum from 9.1 to 13.1psi @14:57:13.83 SS.reconfigure SSinhale.with maxSpeed: 39 @14:57:13.92 SS.seek 25ml @14:57:59.94 Stroke completed -- waiting for pressure to equalize ... @14:58:04.40 Intake.select :close @14:58:04.58 SSV.dial :exhaust @14:58:05.45 Exhaust.select :open @14:58:05.59 SS.reconfigure SSexhale @14:58:05.66 SS.seek 0ml @14:58:14.99 Exhaust.select :close @14:58:15.14 SSV.dial CBV @14:58:15.17
CBV.dial SSV @14:58:16.07 Allowing 130s for 25.0ml of remaining 1300.0ml @14:58:16.09 Intake.select :open @14:58:18.30 Intake @15.6psia, vacuum from 9.1 to 13.1psi @14:58:18.32 SS.reconfigure SSinhale.with maxSpeed: 39 @14:58:18.41 SS.seek 25ml @14:59:04.46 Stroke completed -- waiting for pressure to equalize ... @14:59:08.93 Intake.select :close @14:59:09.12 SSV.dial :exhaust @14:59:10.01 Exhaust.select :open @14:59:10.16 SS.reconfigure SSexhale @14:59:10.23 SS.seek 0ml @14:59:19.88 Exhaust.select :close @14:59:20.03 SSV.dial CBV @14:59:20.06
CBV.dial SSV @14:59:20.94 Allowing 130s for 25.0ml of remaining 1275.0ml @14:59:20.96 Intake.select :open @14:59:23.17 Intake @15.5psia, vacuum from 9.0 to 13.0psi @14:59:23.20 SS.reconfigure SSinhale.with maxSpeed: 39 @14:59:23.29 SS.seek 25ml @15:00:08.66 Stroke completed -- waiting for pressure to equalize ... @15:00:12.50 Intake.select :close @15:00:12.68 SSV.dial :exhaust @15:00:13.55 Exhaust.select :open @15:00:13.70 SS.reconfigure SSexhale @15:00:13.77 SS.seek 0ml @15:00:23.02 Exhaust.select :close @15:00:23.16 SSV.dial CBV @15:00:23.70
CBV.dial SSV @15:00:24.55 Allowing 130s for 25.0ml of remaining 1250.0ml @15:00:24.57 Intake.select :open @15:00:26.78 Intake @15.6psia, vacuum from 9.1 to 13.1psi @15:00:26.80 SS.reconfigure SSinhale.with maxSpeed: 39 @15:00:26.90 SS.seek 25ml @15:01:12.97 Stroke completed -- waiting for pressure to equalize ... @15:01:17.52 Intake.select :close @15:01:17.70 SSV.dial :exhaust @15:01:18.61 Exhaust.select :open @15:01:18.75 SS.reconfigure SSexhale @15:01:18.82 SS.seek 0ml @15:01:28.10 Exhaust.select :close @15:01:28.26 SSV.dial CBV @15:01:28.29
CBV.dial SSV @15:01:29.18 Allowing 130s for 25.0ml of remaining 1225.0ml @15:01:29.20 Intake.select :open @15:01:31.41 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:01:31.43 SS.reconfigure SSinhale.with maxSpeed: 39 @15:01:31.52 SS.seek 25ml @15:02:17.60 Stroke completed -- waiting for pressure to equalize ... @15:02:21.44 Intake.select :close @15:02:21.63 SSV.dial :exhaust @15:02:22.52 Exhaust.select :open @15:02:22.67 SS.reconfigure SSexhale @15:02:22.74 SS.seek 0ml @15:02:32.38 Exhaust.select :close @15:02:32.53 SSV.dial CBV @15:02:32.56
CBV.dial SSV @15:02:33.44 Allowing 130s for 25.0ml of remaining 1200.0ml @15:02:33.46 Intake.select :open @15:02:35.66 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:02:35.69 SS.reconfigure SSinhale.with maxSpeed: 39 @15:02:35.78 SS.seek 25ml @15:03:21.13 Stroke completed -- waiting for pressure to equalize ... @15:03:24.98 Intake.select :close @15:03:25.17 SSV.dial :exhaust @15:03:26.04 Exhaust.select :open @15:03:26.18 SS.reconfigure SSexhale @15:03:26.30 SS.seek 0ml @15:03:35.52 Exhaust.select :close @15:03:35.67 SSV.dial CBV @15:03:35.70
CBV.dial SSV @15:03:36.58 Allowing 130s for 25.0ml of remaining 1175.0ml @15:03:36.60 Intake.select :open @15:03:38.81 Intake @15.6psia, vacuum from 9.1 to 13.1psi @15:03:38.83 SS.reconfigure SSinhale.with maxSpeed: 39 @15:03:38.92 SS.seek 25ml @15:04:24.44 Stroke completed -- waiting for pressure to equalize ... @15:04:28.28 Intake.select :close @15:04:28.45 SSV.dial :exhaust @15:04:29.32 Exhaust.select :open @15:04:29.46 SS.reconfigure SSexhale @15:04:29.54 SS.seek 0ml @15:04:39.14 Exhaust.select :close @15:04:39.28 SSV.dial CBV @15:04:39.31
CBV.dial SSV @15:04:40.21 Allowing 130s for 25.0ml of remaining 1150.0ml @15:04:40.23 Intake.select :open @15:04:42.44 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:04:42.46 SS.reconfigure SSinhale.with maxSpeed: 39 @15:04:43.04 SS.seek 25ml @15:05:28.46 Stroke completed -- waiting for pressure to equalize ... @15:05:32.32 Intake.select :close @15:05:32.51 SSV.dial :exhaust @15:05:33.39 Exhaust.select :open @15:05:33.53 SS.reconfigure SSexhale @15:05:33.62 SS.seek 0ml @15:05:43.21 Exhaust.select :close @15:05:43.36 SSV.dial CBV @15:05:43.39
CBV.dial SSV @15:05:44.29 Allowing 130s for 25.0ml of remaining 1125.0ml @15:05:44.31 Intake.select :open @15:05:46.52 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:05:46.54 SS.reconfigure SSinhale.with maxSpeed: 39 @15:05:46.63 SS.seek 25ml @15:06:32.07 Stroke completed -- waiting for pressure to equalize ... @15:06:35.91 Intake.select :close @15:06:36.08 SSV.dial :exhaust @15:06:36.97 Exhaust.select :open @15:06:37.11 SS.reconfigure SSexhale @15:06:37.19 SS.seek 0ml @15:06:46.42 Exhaust.select :close @15:06:46.57 SSV.dial CBV @15:06:46.60
CBV.dial SSV @15:06:47.49 Allowing 130s for 25.0ml of remaining 1100.0ml @15:06:47.51 Intake.select :open @15:06:49.71 Intake @15.6psia, vacuum from 9.1 to 13.1psi @15:06:49.74 SS.reconfigure SSinhale.with maxSpeed: 39 @15:06:49.83 SS.seek 25ml @15:07:35.89 Stroke completed -- waiting for pressure to equalize ... @15:07:39.74 Intake.select :close @15:07:39.93 SSV.dial :exhaust @15:07:40.80 Exhaust.select :open @15:07:40.94 SS.reconfigure SSexhale @15:07:41.02 SS.seek 0ml @15:07:50.85 Exhaust.select :close @15:07:51.00 SSV.dial CBV @15:07:51.03
CBV.dial SSV @15:07:51.92 Allowing 130s for 25.0ml of remaining 1075.0ml @15:07:51.94 Intake.select :open @15:07:54.15 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:07:54.17 SS.reconfigure SSinhale.with maxSpeed: 39 @15:07:54.26 SS.seek 25ml @15:08:39.50 Stroke completed -- waiting for pressure to equalize ... @15:08:42.75 Intake.select :close @15:08:42.94 SSV.dial :exhaust @15:08:43.82 Exhaust.select :open @15:08:43.96 SS.reconfigure SSexhale @15:08:44.03 SS.seek 0ml @15:08:53.58 Exhaust.select :close @15:08:53.72 SSV.dial CBV @15:08:53.75
CBV.dial SSV @15:08:54.64 Allowing 130s for 25.0ml of remaining 1050.0ml @15:08:54.66 Intake.select :open @15:08:56.87 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:08:56.89 SS.reconfigure SSinhale.with maxSpeed: 39 @15:08:57.46 SS.seek 25ml @15:09:42.96 Stroke completed -- waiting for pressure to equalize ... @15:09:46.82 Intake.select :close @15:09:47.01 SSV.dial :exhaust @15:09:47.89 Exhaust.select :open @15:09:48.03 SS.reconfigure SSexhale @15:09:48.11 SS.seek 0ml @15:09:57.75 Exhaust.select :close @15:09:57.88 SSV.dial CBV @15:09:57.92
CBV.dial SSV @15:09:58.78 Allowing 130s for 25.0ml of remaining 1025.0ml @15:09:58.80 Intake.select :open @15:10:01.01 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:10:01.04 SS.reconfigure SSinhale.with maxSpeed: 39 @15:10:01.14 SS.seek 25ml @15:10:46.85 Stroke completed -- waiting for pressure to equalize ... @15:10:50.71 Intake.select :close @15:10:50.89 SSV.dial :exhaust @15:10:51.77 Exhaust.select :open @15:10:51.91 SS.reconfigure SSexhale @15:10:51.99 SS.seek 0ml @15:11:01.49 Exhaust.select :close @15:11:01.63 SSV.dial CBV @15:11:01.66
CBV.dial SSV @15:11:02.55 Allowing 130s for 25.0ml of remaining 1000.0ml @15:11:02.57 Intake.select :open @15:11:04.77 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:11:04.80 SS.reconfigure SSinhale.with maxSpeed: 39 @15:11:04.88 SS.seek 25ml @15:11:50.36 Stroke completed -- waiting for pressure to equalize ... @15:11:54.23 Intake.select :close @15:11:54.42 SSV.dial :exhaust @15:11:55.28 Exhaust.select :open @15:11:55.42 SS.reconfigure SSexhale @15:11:55.50 SS.seek 0ml @15:12:04.78 Exhaust.select :close @15:12:04.93 SSV.dial CBV @15:12:04.96
CBV.dial SSV @15:12:05.86 Allowing 130s for 25.0ml of remaining 975.0ml @15:12:05.88 Intake.select :open @15:12:08.10 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:12:08.12 SS.reconfigure SSinhale.with maxSpeed: 39 @15:12:08.21 SS.seek 25ml @15:12:53.70 Stroke completed -- waiting for pressure to equalize ... @15:12:57.55 Intake.select :close @15:12:57.72 SSV.dial :exhaust @15:12:58.61 Exhaust.select :open @15:12:58.75 SS.reconfigure SSexhale @15:12:58.83 SS.seek 0ml @15:13:08.64 Exhaust.select :close @15:13:08.79 SSV.dial CBV @15:13:08.82
CBV.dial SSV @15:13:09.70 Allowing 130s for 25.0ml of remaining 950.0ml @15:13:09.72 Intake.select :open @15:13:12.32 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:13:12.42 SS.reconfigure SSinhale.with maxSpeed: 39 @15:13:12.52 SS.seek 25ml @15:13:57.80 Stroke completed -- waiting for pressure to equalize ... @15:14:01.03 Intake.select :close @15:14:01.21 SSV.dial :exhaust @15:14:02.08 Exhaust.select :open @15:14:02.22 SS.reconfigure SSexhale @15:14:02.29 SS.seek 0ml @15:14:11.89 Exhaust.select :close @15:14:12.04 SSV.dial CBV @15:14:12.07
CBV.dial SSV @15:14:12.95 Allowing 130s for 25.0ml of remaining 925.0ml @15:14:12.97 Intake.select :open @15:14:15.17 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:14:15.20 SS.reconfigure SSinhale.with maxSpeed: 39 @15:14:15.29 SS.seek 25ml @15:15:00.70 Stroke completed -- waiting for pressure to equalize ... @15:15:03.95 Intake.select :close @15:15:04.14 SSV.dial :exhaust @15:15:05.02 Exhaust.select :open @15:15:05.16 SS.reconfigure SSexhale @15:15:05.23 SS.seek 0ml @15:15:14.47 Exhaust.select :close @15:15:14.62 SSV.dial CBV @15:15:14.65
CBV.dial SSV @15:15:15.55 Allowing 130s for 25.0ml of remaining 900.0ml @15:15:15.57 Intake.select :open @15:15:17.78 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:15:17.80 SS.reconfigure SSinhale.with maxSpeed: 39 @15:15:17.90 SS.seek 25ml @15:16:03.91 Stroke completed -- waiting for pressure to equalize ... @15:16:07.75 Intake.select :close @15:16:07.93 SSV.dial :exhaust @15:16:08.81 Exhaust.select :open @15:16:08.96 SS.reconfigure SSexhale @15:16:09.05 SS.seek 0ml @15:16:18.34 Exhaust.select :close @15:16:18.49 SSV.dial CBV @15:16:18.52
CBV.dial SSV @15:16:19.41 Allowing 130s for 25.0ml of remaining 875.0ml @15:16:19.43 Intake.select :open @15:16:21.64 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:16:21.67 SS.reconfigure SSinhale.with maxSpeed: 39 @15:16:21.76 SS.seek 25ml @15:17:08.09 Stroke completed -- waiting for pressure to equalize ... @15:17:11.31 Intake.select :close @15:17:11.49 SSV.dial :exhaust @15:17:12.38 Exhaust.select :open @15:17:12.52 SS.reconfigure SSexhale @15:17:12.59 SS.seek 0ml @15:17:21.87 Exhaust.select :close @15:17:22.02 SSV.dial CBV @15:17:22.05
CBV.dial SSV @15:17:22.94 Allowing 130s for 25.0ml of remaining 850.0ml @15:17:22.96 Intake.select :open @15:17:25.17 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:17:25.19 SS.reconfigure SSinhale.with maxSpeed: 39 @15:17:25.28 SS.seek 25ml @15:18:11.34 Stroke completed -- waiting for pressure to equalize ... @15:18:15.03 Intake.select :close @15:18:15.21 SSV.dial :exhaust @15:18:16.08 Exhaust.select :open @15:18:16.22 SS.reconfigure SSexhale @15:18:16.29 SS.seek 0ml @15:18:25.92 Exhaust.select :close @15:18:26.07 SSV.dial CBV @15:18:26.10
CBV.dial SSV @15:18:26.98 Allowing 130s for 25.0ml of remaining 825.0ml @15:18:27.00 Intake.select :open @15:18:29.21 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:18:29.23 SS.reconfigure SSinhale.with maxSpeed: 39 @15:18:29.32 SS.seek 25ml @15:19:14.75 Stroke completed -- waiting for pressure to equalize ... @15:19:18.61 Intake.select :close @15:19:18.79 SSV.dial :exhaust @15:19:19.66 Exhaust.select :open @15:19:19.80 SS.reconfigure SSexhale @15:19:19.88 SS.seek 0ml @15:19:29.78 Exhaust.select :close @15:19:29.93 SSV.dial CBV @15:19:29.96
CBV.dial SSV @15:19:30.86 Allowing 130s for 25.0ml of remaining 800.0ml @15:19:30.88 Intake.select :open @15:19:33.09 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:19:33.10 SS.reconfigure SSinhale.with maxSpeed: 39 @15:19:33.20 SS.seek 25ml @15:20:18.25 Stroke completed -- waiting for pressure to equalize ... @15:20:21.47 Intake.select :close @15:20:21.64 SSV.dial :exhaust @15:20:22.51 Exhaust.select :open @15:20:22.65 SS.reconfigure SSexhale @15:20:22.72 SS.seek 0ml @15:20:32.31 Exhaust.select :close @15:20:32.45 SSV.dial CBV @15:20:32.48
CBV.dial SSV @15:20:33.36 Allowing 130s for 25.0ml of remaining 775.0ml @15:20:33.38 Intake.select :open @15:20:35.59 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:20:35.61 SS.reconfigure SSinhale.with maxSpeed: 39 @15:20:35.70 SS.seek 25ml @15:21:21.15 Stroke completed -- waiting for pressure to equalize ... @15:21:25.01 Intake.select :close @15:21:25.20 SSV.dial :exhaust @15:21:26.08 Exhaust.select :open @15:21:26.23 SS.reconfigure SSexhale @15:21:26.31 SS.seek 0ml @15:21:35.58 Exhaust.select :close @15:21:35.73 SSV.dial CBV @15:21:35.76
CBV.dial SSV @15:21:36.65 Allowing 130s for 25.0ml of remaining 750.0ml @15:21:36.67 Intake.select :open @15:21:38.87 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:21:38.90 SS.reconfigure SSinhale.with maxSpeed: 39 @15:21:38.99 SS.seek 25ml @15:22:25.01 Stroke completed -- waiting for pressure to equalize ... @15:22:28.72 Intake.select :close @15:22:28.90 SSV.dial :exhaust @15:22:29.80 Exhaust.select :open @15:22:29.94 SS.reconfigure SSexhale @15:22:30.02 SS.seek 0ml @15:22:39.79 Exhaust.select :close @15:22:39.94 SSV.dial CBV @15:22:39.97
CBV.dial SSV @15:22:40.86 Allowing 130s for 25.0ml of remaining 725.0ml @15:22:40.88 Intake.select :open @15:22:43.09 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:22:43.11 SS.reconfigure SSinhale.with maxSpeed: 39 @15:22:43.20 SS.seek 25ml @15:23:28.75 Stroke completed -- waiting for pressure to equalize ... @15:23:32.63 Intake.select :close @15:23:32.81 SSV.dial :exhaust @15:23:33.69 Exhaust.select :open @15:23:33.84 SS.reconfigure SSexhale @15:23:33.92 SS.seek 0ml @15:23:43.15 Exhaust.select :close @15:23:43.28 SSV.dial CBV @15:23:43.31
CBV.dial SSV @15:23:44.22 Allowing 130s for 25.0ml of remaining 700.0ml @15:23:44.24 Intake.select :open @15:23:46.46 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:23:46.48 SS.reconfigure SSinhale.with maxSpeed: 39 @15:23:46.58 SS.seek 25ml @15:24:32.62 Stroke completed -- waiting for pressure to equalize ... @15:24:36.48 Intake.select :close @15:24:36.66 SSV.dial :exhaust @15:24:37.54 Exhaust.select :open @15:24:37.69 SS.reconfigure SSexhale @15:24:37.76 SS.seek 0ml @15:24:47.01 Exhaust.select :close @15:24:47.16 SSV.dial CBV @15:24:47.19
CBV.dial SSV @15:24:48.08 Allowing 130s for 25.0ml of remaining 675.0ml @15:24:48.10 Intake.select :open @15:24:50.31 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:24:50.33 SS.reconfigure SSinhale.with maxSpeed: 39 @15:24:50.42 SS.seek 25ml @15:25:36.40 Stroke completed -- waiting for pressure to equalize ... @15:25:40.26 Intake.select :close @15:25:40.44 SSV.dial :exhaust @15:25:41.31 Exhaust.select :open @15:25:41.45 SS.reconfigure SSexhale @15:25:41.53 SS.seek 0ml @15:25:50.76 Exhaust.select :close @15:25:50.89 SSV.dial CBV @15:25:50.92
CBV.dial SSV @15:25:51.83 Allowing 130s for 25.0ml of remaining 650.0ml @15:25:51.85 Intake.select :open @15:25:54.06 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:25:54.08 SS.reconfigure SSinhale.with maxSpeed: 39 @15:25:54.17 SS.seek 25ml @15:26:40.22 Stroke completed -- waiting for pressure to equalize ... @15:26:43.91 Intake.select :close @15:26:44.08 SSV.dial :exhaust @15:26:44.95 Exhaust.select :open @15:26:45.09 SS.reconfigure SSexhale @15:26:45.16 SS.seek 0ml @15:26:54.81 Exhaust.select :close @15:26:54.96 SSV.dial CBV @15:26:54.99
CBV.dial SSV @15:26:55.87 Allowing 130s for 25.0ml of remaining 625.0ml @15:26:55.89 Intake.select :open @15:26:58.10 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:26:58.13 SS.reconfigure SSinhale.with maxSpeed: 39 @15:26:58.22 SS.seek 25ml @15:27:43.59 Stroke completed -- waiting for pressure to equalize ... @15:27:46.83 Intake.select :close @15:27:47.02 SSV.dial :exhaust @15:27:47.90 Exhaust.select :open @15:27:48.04 SS.reconfigure SSexhale @15:27:48.11 SS.seek 0ml @15:27:57.67 Exhaust.select :close @15:27:57.81 SSV.dial CBV @15:27:57.84
CBV.dial SSV @15:27:58.73 Allowing 130s for 25.0ml of remaining 600.0ml @15:27:58.75 Intake.select :open @15:28:00.96 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:28:00.98 SS.reconfigure SSinhale.with maxSpeed: 39 @15:28:01.07 SS.seek 25ml @15:28:46.48 Stroke completed -- waiting for pressure to equalize ... @15:28:50.33 Intake.select :close @15:28:50.52 SSV.dial :exhaust @15:28:51.39 Exhaust.select :open @15:28:51.53 SS.reconfigure SSexhale @15:28:51.61 SS.seek 0ml @15:29:00.82 Exhaust.select :close @15:29:00.96 SSV.dial CBV @15:29:00.99
CBV.dial SSV @15:29:01.89 Allowing 130s for 25.0ml of remaining 575.0ml @15:29:01.91 Intake.select :open @15:29:04.12 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:29:04.14 SS.reconfigure SSinhale.with maxSpeed: 39 @15:29:04.23 SS.seek 25ml @15:29:50.21 Stroke completed -- waiting for pressure to equalize ... @15:29:54.04 Intake.select :close @15:29:54.22 SSV.dial :exhaust @15:29:55.09 Exhaust.select :open @15:29:55.22 SS.reconfigure SSexhale @15:29:55.30 SS.seek 0ml @15:30:04.53 Exhaust.select :close @15:30:04.68 SSV.dial CBV @15:30:04.71
CBV.dial SSV @15:30:05.59 Allowing 130s for 25.0ml of remaining 550.0ml @15:30:05.61 Intake.select :open @15:30:07.81 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:30:07.84 SS.reconfigure SSinhale.with maxSpeed: 39 @15:30:07.93 SS.seek 25ml @15:30:53.98 Stroke completed -- waiting for pressure to equalize ... @15:30:57.69 Intake.select :close @15:30:57.88 SSV.dial :exhaust @15:30:58.76 Exhaust.select :open @15:30:58.90 SS.reconfigure SSexhale @15:30:58.97 SS.seek 0ml @15:31:08.21 Exhaust.select :close @15:31:08.35 SSV.dial CBV @15:31:08.38
CBV.dial SSV @15:31:09.28 Allowing 130s for 25.0ml of remaining 525.0ml @15:31:09.30 Intake.select :open @15:31:11.51 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:31:11.53 SS.reconfigure SSinhale.with maxSpeed: 39 @15:31:11.62 SS.seek 25ml @15:31:57.64 Stroke completed -- waiting for pressure to equalize ... @15:32:01.50 Intake.select :close @15:32:01.68 SSV.dial :exhaust @15:32:02.57 Exhaust.select :open @15:32:02.71 SS.reconfigure SSexhale @15:32:02.78 SS.seek 0ml @15:32:12.45 Exhaust.select :close @15:32:12.59 SSV.dial CBV @15:32:12.62
CBV.dial SSV @15:32:13.51 Allowing 130s for 25.0ml of remaining 500.0ml @15:32:13.53 Intake.select :open @15:32:15.74 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:32:15.76 SS.reconfigure SSinhale.with maxSpeed: 39 @15:32:15.85 SS.seek 25ml @15:33:01.15 Stroke completed -- waiting for pressure to equalize ... @15:33:05.03 Intake.select :close @15:33:05.21 SSV.dial :exhaust @15:33:06.09 Exhaust.select :open @15:33:06.23 SS.reconfigure SSexhale @15:33:06.31 SS.seek 0ml @15:33:15.53 Exhaust.select :close @15:33:15.68 SSV.dial CBV @15:33:15.71
CBV.dial SSV @15:33:16.60 Allowing 130s for 25.0ml of remaining 475.0ml @15:33:16.62 Intake.select :open @15:33:18.83 Intake @15.3psia, vacuum from 8.8 to 12.8psi @15:33:18.85 SS.reconfigure SSinhale.with maxSpeed: 39 @15:33:18.94 SS.seek 25ml @15:34:05.03 Stroke completed -- waiting for pressure to equalize ... @15:34:08.90 Intake.select :close @15:34:09.07 SSV.dial :exhaust @15:34:09.95 Exhaust.select :open @15:34:10.09 SS.reconfigure SSexhale @15:34:10.17 SS.seek 0ml @15:34:19.93 Exhaust.select :close @15:34:20.06 SSV.dial CBV @15:34:20.09
CBV.dial SSV @15:34:20.98 Allowing 130s for 25.0ml of remaining 450.0ml @15:34:21.00 Intake.select :open @15:34:23.20 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:34:23.23 SS.reconfigure SSinhale.with maxSpeed: 39 @15:34:23.32 SS.seek 25ml @15:35:08.57 Stroke completed -- waiting for pressure to equalize ... @15:35:12.26 Intake.select :close @15:35:12.44 SSV.dial :exhaust @15:35:13.31 Exhaust.select :open @15:35:13.45 SS.reconfigure SSexhale @15:35:13.52 SS.seek 0ml @15:35:23.12 Exhaust.select :close @15:35:23.27 SSV.dial CBV @15:35:23.30
CBV.dial SSV @15:35:24.20 Allowing 130s for 25.0ml of remaining 425.0ml @15:35:24.22 Intake.select :open @15:35:26.44 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:35:26.46 SS.reconfigure SSinhale.with maxSpeed: 39 @15:35:26.55 SS.seek 25ml @15:36:12.04 Stroke completed -- waiting for pressure to equalize ... @15:36:15.93 Intake.select :close @15:36:16.11 SSV.dial :exhaust @15:36:16.99 Exhaust.select :open @15:36:17.13 SS.reconfigure SSexhale @15:36:17.22 SS.seek 0ml @15:36:27.01 Exhaust.select :close @15:36:27.15 SSV.dial CBV @15:36:27.18
CBV.dial SSV @15:36:28.06 Allowing 130s for 25.0ml of remaining 400.0ml @15:36:28.08 Intake.select :open @15:36:30.29 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:36:30.31 SS.reconfigure SSinhale.with maxSpeed: 39 @15:36:30.41 SS.seek 25ml @15:37:15.74 Stroke completed -- waiting for pressure to equalize ... @15:37:18.95 Intake.select :close @15:37:19.14 SSV.dial :exhaust @15:37:20.01 Exhaust.select :open @15:37:20.16 SS.reconfigure SSexhale @15:37:20.23 SS.seek 0ml @15:37:29.52 Exhaust.select :close @15:37:29.67 SSV.dial CBV @15:37:29.70
CBV.dial SSV @15:37:30.60 Allowing 130s for 25.0ml of remaining 375.0ml @15:37:30.62 Intake.select :open @15:37:32.82 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:37:32.85 SS.reconfigure SSinhale.with maxSpeed: 39 @15:37:32.94 SS.seek 25ml @15:38:19.02 Stroke completed -- waiting for pressure to equalize ... @15:38:22.88 Intake.select :close @15:38:23.06 SSV.dial :exhaust @15:38:23.95 Exhaust.select :open @15:38:24.09 SS.reconfigure SSexhale @15:38:24.17 SS.seek 0ml @15:38:33.76 Exhaust.select :close @15:38:33.91 SSV.dial CBV @15:38:33.94
CBV.dial SSV @15:38:34.82 Allowing 130s for 25.0ml of remaining 350.0ml @15:38:34.84 Intake.select :open @15:38:37.05 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:38:37.07 SS.reconfigure SSinhale.with maxSpeed: 39 @15:38:37.17 SS.seek 25ml @15:39:22.59 Stroke completed -- waiting for pressure to equalize ... @15:39:26.31 Intake.select :close @15:39:26.49 SSV.dial :exhaust @15:39:27.37 Exhaust.select :open @15:39:27.51 SS.reconfigure SSexhale @15:39:27.58 SS.seek 0ml @15:39:37.16 Exhaust.select :close @15:39:37.29 SSV.dial CBV @15:39:37.32
CBV.dial SSV @15:39:38.21 Allowing 130s for 25.0ml of remaining 325.0ml @15:39:38.23 Intake.select :open @15:39:40.44 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:39:40.46 SS.reconfigure SSinhale.with maxSpeed: 39 @15:39:40.55 SS.seek 25ml @15:40:26.10 Stroke completed -- waiting for pressure to equalize ... @15:40:29.98 Intake.select :close @15:40:30.17 SSV.dial :exhaust @15:40:31.05 Exhaust.select :open @15:40:31.19 SS.reconfigure SSexhale @15:40:31.27 SS.seek 0ml @15:40:40.80 Exhaust.select :close @15:40:40.95 SSV.dial CBV @15:40:40.98
CBV.dial SSV @15:40:41.87 Allowing 130s for 25.0ml of remaining 300.0ml @15:40:41.89 Intake.select :open @15:40:44.10 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:40:44.12 SS.reconfigure SSinhale.with maxSpeed: 39 @15:40:44.21 SS.seek 25ml @15:41:29.68 Stroke completed -- waiting for pressure to equalize ... @15:41:33.50 Intake.select :close @15:41:33.68 SSV.dial :exhaust @15:41:34.55 Exhaust.select :open @15:41:34.70 SS.reconfigure SSexhale @15:41:34.78 SS.seek 0ml @15:41:44.02 Exhaust.select :close @15:41:44.16 SSV.dial CBV @15:41:44.19
CBV.dial SSV @15:41:45.07 Allowing 130s for 25.0ml of remaining 275.0ml @15:41:45.09 Intake.select :open @15:41:47.29 Intake @15.3psia, vacuum from 8.8 to 12.8psi @15:41:47.32 SS.reconfigure SSinhale.with maxSpeed: 39 @15:41:47.41 SS.seek 25ml @15:42:33.52 Stroke completed -- waiting for pressure to equalize ... @15:42:37.35 Intake.select :close @15:42:37.55 SSV.dial :exhaust @15:42:38.43 Exhaust.select :open @15:42:38.57 SS.reconfigure SSexhale @15:42:38.65 SS.seek 0ml @15:42:48.33 Exhaust.select :close @15:42:48.48 SSV.dial CBV @15:42:48.51
CBV.dial SSV @15:42:49.38 Allowing 130s for 25.0ml of remaining 250.0ml @15:42:49.40 Intake.select :open @15:42:51.61 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:42:51.63 SS.reconfigure SSinhale.with maxSpeed: 39 @15:42:51.72 SS.seek 25ml @15:43:37.01 Stroke completed -- waiting for pressure to equalize ... @15:43:40.70 Intake.select :close @15:43:40.88 SSV.dial :exhaust @15:43:41.76 Exhaust.select :open @15:43:41.90 SS.reconfigure SSexhale @15:43:41.97 SS.seek 0ml @15:43:51.66 Exhaust.select :close @15:43:51.79 SSV.dial CBV @15:43:51.85
CBV.dial SSV @15:43:52.69 Allowing 130s for 25.0ml of remaining 225.0ml @15:43:52.71 Intake.select :open @15:43:54.92 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:43:54.94 SS.reconfigure SSinhale.with maxSpeed: 39 @15:43:55.04 SS.seek 25ml @15:44:40.69 Stroke completed -- waiting for pressure to equalize ... @15:44:44.56 Intake.select :close @15:44:44.74 SSV.dial :exhaust @15:44:45.63 Exhaust.select :open @15:44:45.77 SS.reconfigure SSexhale @15:44:45.85 SS.seek 0ml @15:44:55.51 Exhaust.select :close @15:44:55.65 SSV.dial CBV @15:44:55.68
CBV.dial SSV @15:44:56.57 Allowing 130s for 25.0ml of remaining 200.0ml @15:44:56.59 Intake.select :open @15:44:58.80 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:44:58.82 SS.reconfigure SSinhale.with maxSpeed: 39 @15:44:58.92 SS.seek 25ml @15:45:44.24 Stroke completed -- waiting for pressure to equalize ... @15:45:48.10 Intake.select :close @15:45:48.29 SSV.dial :exhaust @15:45:49.16 Exhaust.select :open @15:45:49.30 SS.reconfigure SSexhale @15:45:49.38 SS.seek 0ml @15:45:59.08 Exhaust.select :close @15:45:59.22 SSV.dial CBV @15:45:59.25
CBV.dial SSV @15:46:00.13 Allowing 130s for 25.0ml of remaining 175.0ml @15:46:00.15 Intake.select :open @15:46:02.36 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:46:02.38 SS.reconfigure SSinhale.with maxSpeed: 39 @15:46:02.48 SS.seek 25ml @15:46:47.80 Stroke completed -- waiting for pressure to equalize ... @15:46:51.04 Intake.select :close @15:46:51.23 SSV.dial :exhaust @15:46:52.10 Exhaust.select :open @15:46:52.23 SS.reconfigure SSexhale @15:46:52.30 SS.seek 0ml @15:47:01.60 Exhaust.select :close @15:47:01.74 SSV.dial CBV @15:47:01.77
CBV.dial SSV @15:47:02.68 Allowing 130s for 25.0ml of remaining 150.0ml @15:47:02.70 Intake.select :open @15:47:04.92 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:47:04.95 SS.reconfigure SSinhale.with maxSpeed: 39 @15:47:05.04 SS.seek 25ml @15:47:51.15 Stroke completed -- waiting for pressure to equalize ... @15:47:55.48 Intake.select :close @15:47:55.67 SSV.dial :exhaust @15:47:56.55 Exhaust.select :open @15:47:56.69 SS.reconfigure SSexhale @15:47:56.76 SS.seek 0ml @15:48:05.97 Exhaust.select :close @15:48:06.12 SSV.dial CBV @15:48:06.15
CBV.dial SSV @15:48:07.04 Allowing 130s for 25.0ml of remaining 125.0ml @15:48:07.06 Intake.select :open @15:48:09.27 Intake @15.2psia, vacuum from 8.7 to 12.7psi @15:48:09.29 SS.reconfigure SSinhale.with maxSpeed: 39 @15:48:09.38 SS.seek 25ml @15:48:55.72 Stroke completed -- waiting for pressure to equalize ... @15:48:59.60 Intake.select :close @15:48:59.78 SSV.dial :exhaust @15:49:00.65 Exhaust.select :open @15:49:00.79 SS.reconfigure SSexhale @15:49:00.87 SS.seek 0ml @15:49:10.50 Exhaust.select :close @15:49:10.65 SSV.dial CBV @15:49:10.68
CBV.dial SSV @15:49:11.58 Allowing 130s for 25.0ml of remaining 100.0ml @15:49:11.60 Intake.select :open @15:49:13.81 Intake @15.4psia, vacuum from 8.9 to 12.9psi @15:49:13.83 SS.reconfigure SSinhale.with maxSpeed: 39 @15:49:13.92 SS.seek 25ml @15:49:59.69 Stroke completed -- waiting for pressure to equalize ... @15:50:02.89 Intake.select :close @15:50:03.08 SSV.dial :exhaust @15:50:03.96 Exhaust.select :open @15:50:04.11 SS.reconfigure SSexhale @15:50:04.18 SS.seek 0ml @15:50:13.93 Exhaust.select :close @15:50:14.08 SSV.dial CBV @15:50:14.11
CBV.dial SSV @15:50:14.99 Allowing 130s for 25.0ml of remaining 74.9ml @15:50:15.01 Intake.select :open @15:50:17.22 Intake @15.3psia, vacuum from 8.8 to 12.8psi @15:50:17.25 SS.reconfigure SSinhale.with maxSpeed: 39 @15:50:17.34 SS.seek 25ml @15:51:02.71 Stroke completed -- waiting for pressure to equalize ... @15:51:06.56 Intake.select :close @15:51:06.74 SSV.dial :exhaust @15:51:07.61 Exhaust.select :open @15:51:07.74 SS.reconfigure SSexhale @15:51:07.84 SS.seek 0ml @15:51:17.36 Exhaust.select :close @15:51:17.50 SSV.dial CBV @15:51:17.52
CBV.dial SSV @15:51:18.43 Allowing 130s for 25.0ml of remaining 49.9ml @15:51:18.45 Intake.select :open @15:51:20.65 Intake @15.3psia, vacuum from 8.8 to 12.8psi @15:51:20.68 SS.reconfigure SSinhale.with maxSpeed: 39 @15:51:20.78 SS.seek 25ml @15:52:06.32 Stroke completed -- waiting for pressure to equalize ... @15:52:10.63 Intake.select :close @15:52:10.82 SSV.dial :exhaust @15:52:11.69 Exhaust.select :open @15:52:11.83 SS.reconfigure SSexhale @15:52:11.90 SS.seek 0ml @15:52:21.19 Exhaust.select :close @15:52:21.34 SSV.dial CBV @15:52:21.37
CBV.dial SSV @15:52:22.25 Allowing 130s for 24.9ml of remaining 24.9ml @15:52:22.27 Intake.select :open @15:52:24.48 Intake @15.3psia, vacuum from 8.8 to 12.8psi @15:52:24.50 SS.reconfigure SSinhale.with maxSpeed: 39 @15:52:24.59 SS.seek 24.95ml @15:53:10.61 Stroke completed -- waiting for pressure to equalize ... @15:53:14.49 Intake.select :close @15:53:14.67 SSV.dial :exhaust @15:53:15.55 Exhaust.select :open @15:53:15.70 SS.reconfigure SSexhale @15:53:15.77 SS.seek 0ml @15:53:25.52 Exhaust.select :close @15:53:25.66 Sampled 1500.0ml @15:53:25.67 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @15:53:25.69 Intake.select :close @15:53:25.80 CSV.dial :waste @15:53:25.88 CS.seek 0ml @15:53:26.00 SSV.dial CBV,avoiding: :exhaust @15:53:26.04
CBV.dial SSV @15:53:26.13 CSV.dial :air @15:53:27.00 CS.seek 10ml @15:53:28.19
CTV.dial :air @15:53:29.07 delay 15 seconds @15:53:44.10 SSV.dial :spit,avoiding: :exhaust @15:53:45.63 CTV.dial CSV,avoiding: :intake @15:53:45.69
SS.seek 0ml @15:53:45.86 SSV.dial CBV,avoiding: :exhaust @15:53:53.82 CBV.dial between SSV and waste2 @15:53:54.35 CTV.dial CSV,avoiding: :air @15:53:54.38
CSV.dial CTV @15:53:54.92 CS.reconfigure CSpressurize @15:53:54.99 CS.seek 0ml @15:54:21.22 CSV.dial :air @15:54:21.76 CS.reconfigure CSfast @15:54:21.83 CS.seek 10ml @15:54:48.51 CSV.dial between air and CTV @15:54:48.90 CS.seek 2.667ml @15:55:08.70 CTV.dial CSV,avoiding: :air @15:55:08.73
CSV.dial CTV @15:55:09.12 CS.reconfigure CSpressurize @15:55:09.19 CS.seek 0ml @15:55:13.88 BubblePoint: Intake @69.6psia, Outlet @14.1psia @15:55:13.89 CBV.dial SSV @15:55:14.29 SS.reconfigure SSconfig @15:55:14.36 SS.seek 10ml @15:55:20.33 delay 15 seconds @15:55:35.47 Evacuated: Intake @41.1psia, Outlet @10.4psia @15:55:35.48 SSV.dial :spit,avoiding: :exhaust @15:55:37.03 SS.reconfigure SSexhale @15:55:37.11 SS.seek 0ml @15:55:42.79 SS.reconfigure SSfast @15:55:42.94 SS.seek 5ml @15:55:46.03 SSV.dial CBV,avoiding: :exhaust @15:55:46.06
CBV.dial SSV @15:55:47.62 CTV.dial :air,avoiding: :intake @15:55:49.17 CSV.dial CTV @15:55:49.23
SS.seek 10ml @15:55:50.21 CSV.dial :air @15:55:50.75 CS.reconfigure CSfast @15:55:50.87 CS.seek 0ml @15:55:52.17
SSV.dial :spit,avoiding: :exhaust @15:55:53.70 SS.reconfigure SSexhale @15:55:53.78 SS.seek 0ml @15:55:54.97 CS.coast @15:55:58.93
SS.reconfigure SSfast @15:55:59.05 SS.seek 5ml @15:56:02.15 SSV.dial CBV,avoiding: :exhaust @15:56:02.18
CBV.dial SSV @15:56:04.70 SSV.dial :spit,avoiding: :exhaust @15:56:06.22 SS.reconfigure SSconfig @15:56:06.29 SS.seek 0ml @15:56:09.42 SS.coast @15:56:09.48 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @15:56:09.50 Intake.select :close @15:56:09.61 CSV.dial :waste @15:56:09.77 SSV.dial CBV,avoiding: :exhaust @15:56:09.81
CBV.dial SSV @15:56:10.54 CS.seek 0ml @15:56:10.63 CSV.dial :air @15:56:11.33
CTV.dial :air @15:56:11.35 delay 15 seconds @15:56:11.62 CS.seek 10ml @15:56:26.38
SSV.dial :spit,avoiding: :exhaust @15:56:27.90 CTV.dial CSV,avoiding: :intake @15:56:27.94
SS.reconfigure SSexhale @15:56:28.08 SS.seek 0ml @15:56:28.17 SSV.dial CBV,avoiding: :exhaust @15:56:38.29 CBV.dial between SSV and waste2 @15:56:38.73 CSV.dial between air and CTV @15:56:39.13 CS.seek 8.211ml @15:56:44.64 CTV.dial CSV,avoiding: :air @15:56:44.67
CSV.dial CTV @15:56:45.06 CS.reconfigure CSpressurize @15:56:45.14 CS.seek 0ml @15:57:06.85 CSV.dial :air @15:57:07.51 CS.reconfigure CSfast @15:57:07.59 CS.seek 10ml @15:57:34.17 CSV.dial between air and CTV @15:57:34.55 CS.seek 3.051ml @15:57:53.14 CTV.dial CSV,avoiding: :air @15:57:53.17
CSV.dial CTV @15:57:53.53 CS.reconfigure CSpressurize @15:57:53.60 CS.seek 0ml @15:58:01.00 BubblePoint: Intake @69.1psia, Outlet @13.7psia @15:58:01.01 CBV.dial SSV @15:58:01.42 SS.reconfigure SSconfig @15:58:01.49 SS.seek 10ml @15:58:06.94 delay 15 seconds @15:58:22.09 Evacuated: Intake @43.0psia, Outlet @13.7psia @15:58:22.10 SSV.dial :spit,avoiding: :exhaust @15:58:24.12 SS.reconfigure SSexhale @15:58:24.20 SS.seek 0ml @15:58:29.88 SS.reconfigure SSfast @15:58:29.96 SS.seek 5ml @15:58:32.64 SSV.dial CBV,avoiding: :exhaust @15:58:32.67
CBV.dial SSV @15:58:34.20 CTV.dial :air,avoiding: :intake @15:58:35.74 CSV.dial CTV @15:58:35.81
SS.seek 10ml @15:58:36.79 CSV.dial :air @15:58:37.44 CS.reconfigure CSfast @15:58:37.51 CS.seek 0ml @15:58:38.35
SSV.dial :spit,avoiding: :exhaust @15:58:39.72 CS.coast @15:58:39.89
SS.reconfigure SSexhale @15:58:39.96 SS.seek 0ml @15:58:45.16 SS.reconfigure SSfast @15:58:45.24 SS.seek 5ml @15:58:48.12 SSV.dial CBV,avoiding: :exhaust @15:58:48.15
CBV.dial SSV @15:58:50.68 SSV.dial :spit,avoiding: :exhaust @15:58:52.20 SS.reconfigure SSconfig @15:58:52.28 SS.seek 0ml @15:58:55.58 SS.coast @15:58:55.65 Lysing: Adding 1.5ml lysis @15:58:55.66 CBV.dial between CSV and waste1,avoiding: :DBV @15:58:57.38 CTV.dial CSV @15:58:58.90 CSV.dial CSR @15:58:59.81 Pulling 1ml of CSR.lysis @15:58:59.82 CSR.select :lysis @15:58:59.98 CS.reconfigure CSconfig @15:59:00.05 CS.seek 1ml @15:59:03.72 delay 5 seconds @15:59:08.75 CSR.select :air @15:59:08.96 CS.seek 6ml @15:59:22.82 delay 2 seconds @15:59:24.84 CSV.dial :waste1 @15:59:26.45 CS.reconfigure CSfast @15:59:26.52 CS.seek :empty @15:59:42.97 CSV.dial CSR @15:59:44.65 Pulling 1.5ml of CSR.lysis @15:59:44.66 CSR.select :lysis @15:59:44.81 CS.reconfigure CSconfig @15:59:44.88 CS.seek 1.5ml @15:59:49.82 delay 5 seconds @15:59:54.85 CSR.select :air @15:59:55.05 CS.seek 4.5ml @16:00:03.72 delay 2 seconds @16:00:05.74 CSV.dial CTV @16:00:06.28 CS.seek 1.275ml @16:00:15.58 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @16:00:15.61 CH.seek 85C,8:00 @16:03:46.94 CH.hold 5:00,85C @16:08:47.13 Collection Puck Heater at 83.4C after lysing @16:08:47.16 CS.seek 0ml @16:08:51.52 CTV.dial between spare and air,avoiding: :intake @16:08:52.73 CSV.dial :air @16:08:53.25 CS.seek 5ml @16:09:05.41 -> exit @16:09:05.43 Session Ended @16:09:07.14
CSV.dial CTV @16:09:07.66 delay 2 seconds @16:09:09.68 CSV.dial :air @16:09:10.31 delay 2 seconds @16:09:12.33 CSV.dial CTV @16:09:12.82 delay 2 seconds @16:09:14.84 CSV.dial :air @16:09:15.46 delay 2 seconds @16:09:17.49 CS.seek 0ml @16:09:31.23 Recovering Lysate into Collection syringe @16:09:31.24 CSV.dial CBV @16:09:32.42 CBV.dial CSV @16:09:32.85 CS.seek 5ml @16:09:46.64 delay 5 seconds @16:09:51.67 CTV.dial :air @16:09:52.25 CS.seek 7ml @16:09:58.35 delay 5 seconds @16:10:03.38 CSV.dial :air2 @16:10:03.90 CS.seek 10ml @16:10:12.48 CSV.dial between air and CTV,avoiding: CBV @16:10:13.80 Lysing Complete @16:10:13.88 Storing sh1 puck in Collection Clamp to tube 1 @16:10:14.07 SC.reconfigure SCtight @16:10:14.12 SC.seek 1 @16:10:16.30 SC.reconfigure SCloose @16:10:16.53 Elbow.reconfigure TightElbow @16:10:17.09 Elbow.seek 1 @16:10:19.11 Forearm.reconfigure TightForearm @16:10:19.16 Forearm.seek 1 + 700 counts @16:10:20.23 SE.reconfigure SEpick @16:10:20.31 SE.seek beamBreak @16:10:23.27 SE.configure SEconfig @16:10:23.33 SE.seek :place @16:10:24.02 Forearm.reconfigure LooseForearm @16:10:24.07 Forearm.seek :retract @16:10:25.01 Elbow.seek CC @16:10:26.69 Elbow.reconfigure LooseElbow @16:10:26.80 Forearm.seek CC @16:10:27.78 Hand.close @16:10:28.52 CC.seek :open @16:10:33.06 Forearm.seek :retract @16:10:34.07 Elbow.reconfigure TightElbow @16:10:34.13 Elbow.seek 1 @16:10:36.03 Elbow.reconfigure LimpElbow @16:10:36.09 Forearm.seek 1 @16:10:37.14 Elbow.hold @16:10:37.22 Elbow.reconfigure TightElbow @16:10:37.27 Hand.open @16:10:38.16 Forearm.force -20 @16:10:38.20 Forearm.seek 1 + 346 counts @16:10:38.54 Forearm.reconfigure TightForearm @16:10:38.58 Forearm.seek 1 + 700 counts @16:10:38.91 SE.seek :down @16:10:40.05
Forearm.reconfigure LooseForearm @16:10:40.10 Forearm.seek :retract @16:10:41.75 SE.coast @16:10:41.79
SC.coast @16:10:41.84 Tranferring lysate to PRV @16:10:41.85 CSV.dial :air @16:10:42.30 CS.seek 10ml @16:10:42.39 CSV.dial between air and CTV @16:10:42.84 AIV.dial :bypass @16:10:42.93 Beginning samplePassC @16:10:42.95 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:10:43.03 Hand.open @16:10:43.40 Elbow.seek FlushPuck::Garage @16:10:45.35 Elbow.reconfigure LooseElbow @16:10:45.46 Forearm.seek :garage @16:10:46.46 Hand.close @16:10:47.39 Forearm.seek :retract @16:10:48.46 Elbow.reconfigure TightElbow @16:10:48.51 Elbow.seek CC @16:10:51.87 Elbow.reconfigure LooseElbow @16:10:51.99 Forearm.reconfigure TightForearm @16:10:52.04 Forearm.seek CC @16:10:53.36 CC.seek :closed @16:10:57.65 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.32 @16:10:57.72 Hand.open @16:10:58.59 Forearm.reconfigure LooseForearm @16:10:58.65 Forearm.seek :retract @16:10:59.65 AWV.select :air @16:10:59.66 AIV.dial :bypass @16:10:59.67 PSV.dial PSR @16:11:01.33 Pulling 1.5ml of PSR.flush @16:11:01.34 PSR.select :flush @16:11:01.47 PS.seek 1.5ml @16:11:17.79 delay 5 seconds @16:11:22.82 PSV.dial :waste1 @16:11:23.47 PS.seek 0ml @16:11:39.82 PSV.dial PSR @16:11:40.30 PSR.select :air @16:11:40.43 PS.seek 2.5ml @16:12:07.08 PRV.dial CBV @16:12:07.11
CBV.dial PRV @16:12:08.01 PSV.dial :mixloop @16:12:09.18 CTV.dial :air,avoiding: :intake @16:12:09.19 PTV.dial :PRVmixing @16:12:09.28 PS.seek 0ml @16:12:35.95 PSV.dial :air @16:12:36.46 PS.seek 2.5ml @16:13:03.04 PSV.dial :mixloop @16:13:03.53 PS.seek 0ml @16:13:29.66 PSV.dial :air @16:13:30.16 PS.seek 2.5ml @16:13:56.63 PSV.dial :mixloop @16:13:57.13 PS.seek 0ml @16:14:23.79 Garaging FlushPuck in Collection Clamp @16:14:23.88 Elbow.reconfigure TightElbow @16:14:23.93 Elbow.seek CC @16:14:24.18 Elbow.reconfigure LooseElbow @16:14:24.28 Forearm.seek CC @16:14:25.30 Hand.close @16:14:26.03 CC.seek :open @16:14:30.42 Forearm.seek :retract @16:14:31.47 Elbow.reconfigure TightElbow @16:14:31.52 Elbow.seek :garage @16:14:34.63 Elbow.reconfigure LooseElbow @16:14:34.73 Forearm.reconfigure TightForearm @16:14:34.78 Forearm.seek :garage @16:14:35.94 Hand.open @16:14:36.71 Forearm.reconfigure LooseForearm @16:14:36.76 Forearm.seek :retract @16:14:37.81 Connecting PS to CS to transfer sample @16:14:37.82 CBV.dial AIV @16:14:37.83 AIV.dial :bypass @16:14:37.85 PSV.dial :mixloop @16:14:37.86 PTV.dial :PRVmixing @16:14:37.87 PRV.dial AIV @16:14:38.38 CTV.dial :air,avoiding: :intake @16:14:38.41 Loading lyfil puck from tube 2 into Collection Clamp @16:14:38.67 SC.reconfigure SCtight @16:14:38.72 SC.seek 2 @16:14:40.90 SC.reconfigure SCloose @16:14:41.14 Elbow.reconfigure TightElbow @16:14:41.20 Elbow.seek 2 @16:14:43.23 Forearm.reconfigure TightForearm @16:14:43.28 Forearm.seek 2 + 700 counts @16:14:44.37 SE.reconfigure SEpick @16:14:44.45 SE.seek beamBreak @16:14:45.57 SE.configure SEconfig @16:14:45.74 Elbow.seek 2 @16:14:45.97 Forearm.seek 2 + 300 counts @16:14:46.25 Forearm.reconfigure LooseForearm @16:14:46.30 Hand.close @16:14:47.04 SE.seek :down @16:14:47.35
Forearm.seek :retract @16:14:48.07 SE.coast @16:14:48.34
Elbow.seek CC @16:14:50.04 Elbow.reconfigure LooseElbow @16:14:50.15 Forearm.reconfigure TightForearm @16:14:50.20 Forearm.seek CC @16:14:51.45 CC.seek :closed @16:14:55.75 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.54 @16:14:55.76 Hand.open @16:14:56.60 Forearm.reconfigure LooseForearm @16:14:56.65 Forearm.seek :retract @16:14:57.63 SC.coast @16:14:57.67 CTV.dial CSV,avoiding: :intake @16:14:59.19 CSV.dial CTV @16:14:59.56 CS.seek 5ml @16:15:13.30 delay 1:20 @16:16:33.34 CS.seek 2.5ml @16:16:40.73 delay 40 seconds @16:17:20.77 CS.seek 0ml @16:17:28.20 delay 1:00 @16:18:28.23 PSV.dial :air @16:18:28.74 PS.seek 2.5ml @16:18:55.42 PSV.dial :mixloop @16:18:55.92 delay 3 seconds @16:18:58.95 PSV.dial :air @16:18:59.45 delay 2 seconds @16:19:01.47 PSV.dial :mixloop @16:19:01.96 delay 3 seconds @16:19:04.99 PSV.dial :waste1 @16:19:06.47 PS.seek :empty @16:19:33.04 CBV.dial :waste1 @16:19:33.56 delay 5 seconds @16:19:38.59 CBV.dial AIV @16:19:39.13 delay 10 seconds @16:19:49.16 CBV.dial :waste1 @16:19:49.69 delay 10 seconds @16:19:59.72 CBV.dial AIV @16:20:00.24 delay 10 seconds @16:20:10.27 CTV.dial CSV,avoiding: :intake @16:20:10.28 CBV.dial between AIV and waste1 @16:20:11.14 CSV.dial :air @16:20:11.70 CS.seek 10ml @16:20:38.24 CSV.dial CTV @16:20:38.76 CS.seek 0ml @16:21:05.42 CBV.dial :waste1 @16:21:05.75 delay 10 seconds @16:21:15.78 CTV.dial :air,avoiding: :intake @16:21:17.34 Storing lyfil puck in Collection Clamp to tube 1 @16:21:17.53 SC.reconfigure SCtight @16:21:17.58 SC.seek 1 @16:21:19.70 SC.reconfigure SCloose @16:21:19.93 Elbow.reconfigure TightElbow @16:21:19.98 Elbow.seek 1 @16:21:21.87 Forearm.reconfigure TightForearm @16:21:21.93 Forearm.seek 1 + 700 counts @16:21:22.98 SE.reconfigure SEpick @16:21:23.06 SE.seek beamBreak @16:21:26.24 SE.configure SEconfig @16:21:26.41 Elbow.reconfigure LimpElbow @16:21:26.46 Forearm.reconfigure LooseForearm @16:21:26.51 Forearm.seek 1 @16:21:26.81 Elbow.hold @16:21:26.88 Elbow.reconfigure TightElbow @16:21:26.99 SE.seek 0.06 Pucks below Top Plate @16:21:27.44 Forearm.force -20 @16:21:27.48 Forearm.seek 1 + 348 counts @16:21:27.80 Forearm.reconfigure TightForearm @16:21:27.86 Forearm.seek 1 + 700 counts @16:21:28.16 SE.seek 1.06 Pucks below Top Plate @16:21:28.76 Forearm.reconfigure LooseForearm @16:21:28.81 Forearm.seek :retract @16:21:29.76 Elbow.seek CC @16:21:31.47 Elbow.reconfigure LooseElbow @16:21:31.57 Forearm.seek CC @16:21:32.55 Hand.close @16:21:33.28 CC.seek :open @16:21:37.82 Forearm.seek :retract @16:21:38.82 Elbow.reconfigure TightElbow @16:21:38.87 Elbow.seek 1 @16:21:40.80 Elbow.reconfigure LimpElbow @16:21:40.85 Forearm.seek 1 @16:21:41.90 Elbow.hold @16:21:41.97 Elbow.reconfigure TightElbow @16:21:42.02 Hand.open @16:21:42.90 Forearm.force -20 @16:21:42.95 Forearm.seek 1 + 342 counts @16:21:43.27 Forearm.reconfigure TightForearm @16:21:43.32 Forearm.seek 1 + 700 counts @16:21:43.69 SE.seek :down @16:21:44.84
Forearm.reconfigure LooseForearm @16:21:44.89 Forearm.seek :retract @16:21:46.43 SE.coast @16:21:46.47
SC.coast @16:21:46.51 PSV.dial :air @16:21:47.73 PS.seek 0.499ml @16:21:53.70 delay 3 seconds @16:21:56.72 PSV.dial :mixloop @16:21:57.20 PRV.dial :air @16:21:57.71 delay 3 seconds @16:22:00.74 PRV.dial CBV @16:22:01.24 CBV.dial AIV @16:22:01.77 PRV.dial between CBV and air @16:22:02.12 PRV.dial :air @16:22:02.53 PS.reconfigure PSslow @16:22:02.58 PS.seek 0.599ml @16:22:05.17 PRV.dial :waste2 @16:22:06.00 PS.reconfigure PSconfig @16:22:06.05 PS.seek :empty @16:22:12.92 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:22:13.00 Hand.open @16:22:13.41 Elbow.seek FlushPuck::Garage @16:22:15.38 Elbow.reconfigure LooseElbow @16:22:15.49 Forearm.seek :garage @16:22:16.52 Hand.close @16:22:17.41 Forearm.seek :retract @16:22:18.46 Elbow.reconfigure TightElbow @16:22:18.51 Elbow.seek CC @16:22:21.45 Elbow.reconfigure LooseElbow @16:22:21.55 Forearm.reconfigure TightForearm @16:22:21.61 Forearm.seek CC @16:22:22.96 CC.seek :closed @16:22:27.25 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.30 @16:22:27.33 Hand.open @16:22:28.09 Forearm.reconfigure LooseForearm @16:22:28.14 Forearm.seek :retract @16:22:29.24 Clearing PML for modified lysate @16:22:29.25 CTV.dial :air,avoiding: :intake @16:22:29.26 PRV.dial :waste2 @16:22:29.28 PTV.dial :PRV,avoiding: :puck @16:22:30.10 delay 2 seconds @16:22:32.12 PTV.dial :PRVmixing,avoiding: :puck @16:22:32.95 delay 2 seconds @16:22:34.98 PSV.dial :PML @16:22:34.99 delay 2 seconds @16:22:37.02 PRV.dial :air @16:22:37.85 delay 2 seconds @16:22:39.94 PS.seek 1.499ml @16:22:56.17 delay 5 seconds @16:23:01.20 PS.seek 2.5ml @16:23:12.20 PSV.dial :air @16:23:12.71 PS.seek 0ml @16:23:39.23 mixing lysate with speDil @16:23:39.24 PSV.dial :PML @16:23:39.73 CBV.dial :PRV @16:23:39.74 PRV.dial :air @16:23:39.76 delay 2 seconds @16:23:41.78 PRV.dial :speDil @16:23:42.81 delay 2 seconds @16:23:44.89 PS.reconfigure PSslow1 @16:23:44.94 PS.seek 0.225ml @16:24:09.26 delay 5 seconds @16:24:14.29 PRV.dial CBV @16:24:15.15 delay 2 seconds @16:24:17.24 PS.seek 0.475ml @16:24:43.14 delay 5 seconds @16:24:48.17 PRV.dial :air @16:24:48.68 delay 2 seconds @16:24:50.72 PS.seek 0.965ml @16:25:40.79 delay 10 seconds @16:25:50.82 PRV.dial CBV @16:25:51.32 CTV.dial :air,avoiding: :intake @16:25:51.34 PSV.dial :air @16:25:51.83 PS.reconfigure PSconfig @16:25:51.88 PS.seek 2.5ml @16:26:08.32 delay 3 seconds @16:26:11.34 PSV.dial PTV @16:26:12.18 PTV.dial :PRV,avoiding: :puck @16:26:13.02 PS.seek 0ml @16:26:39.29 PSV.dial :air @16:26:40.13 PS.seek 2.5ml @16:27:06.39 delay 3 seconds @16:27:09.42 PSV.dial PTV @16:27:10.36 PTV.dial :PRV,avoiding: :puck @16:27:10.39 PS.seek 0ml @16:27:36.70 Pushing modified lysate into AIV Injection Loop @16:27:36.71 CBV.dial AIV @16:27:36.72 AIV.dial :bypass @16:27:36.74 PRV.dial CBV @16:27:36.75 PSV.dial :air @16:27:37.58 PS.seek 2.5ml @16:28:03.79 delay 3 seconds @16:28:06.81 PSV.dial :PML @16:28:07.31 PTV.dial :PRVmixing,avoiding: :puck @16:28:08.21 PS.reconfigure PSslow1 @16:28:08.27 PS.seek 1.74ml @16:29:25.70 delay 5 seconds @16:29:30.73 AIV.dial :sample @16:29:31.37 AWV.select :waste @16:29:31.54 PS.reconfigure PSslow2 @16:29:31.59 PS.seek 1.22ml @16:31:20.11 delay 15 seconds @16:31:35.14 AIV.dial :bypass @16:31:35.78 PS.reconfigure PSconfig @16:31:35.82 PS.seek 0ml @16:31:49.11 PSV.dial :air @16:31:49.63 PS.seek 2.5ml @16:32:15.70 PSV.dial :PML @16:32:16.21 PS.seek 0ml @16:32:42.29 AIV.dial :coilToMFB @16:32:42.30 AWV.select :air @16:32:42.47 Extracting Nucleic Acid from AIV @16:32:42.50
Beginning samplePassD @16:32:42.57 SPE.hold 2:46:40,55C @16:32:42.62 ASV.dial :SPEdelivery @16:32:42.70
AWV.select :air @16:32:42.72 AIV.dial :bypass @16:32:42.73 PSV.dial PSR @16:32:43.05 delay 20 seconds @16:32:44.00
Pulling 1.5ml of PSR.killMFB @16:32:44.01 PSR.select :killMFB @16:32:44.14 PS.seek 1.5ml @16:33:00.98 delay 5 seconds @16:33:03.08 primeLysateINT @16:33:03.09 AWV.select :air @16:33:03.11 AIV.dial :coilToMFB @16:33:03.12 ATV.dial AIV @16:33:05.01 AS.reconfigure ASslow1 @16:33:05.06 AS.seek 0.299ml @16:33:06.01
PSV.dial :waste1 @16:33:06.56 PS.seek 0ml @16:33:23.48 PSV.dial PSR @16:33:24.01 PSR.select :air @16:33:24.14 PS.seek 2.5ml @16:33:51.64 PRV.dial CBV @16:33:51.67
CBV.dial PRV @16:33:52.11 PSV.dial :mixloop @16:33:53.40 CTV.dial :air,avoiding: :intake @16:33:53.42 PTV.dial :PRVmixing @16:33:53.44 PS.seek 0ml @16:34:05.74 delay 10 seconds @16:34:15.77 ATV.dial :air2 @16:34:16.67 AS.seek 0.329ml @16:34:20.84
PSV.dial PSR @16:34:22.13 Pulling 1.5ml of PSR.flush @16:34:22.14 PSR.select :flush @16:34:22.27 PS.seek 1.5ml @16:34:22.99 ATV.dial :waste2 @16:34:23.61 AS.reconfigure ASnorm @16:34:23.65 AS.seek :empty @16:34:39.31
delay 5 seconds @16:34:40.68 Lysate primed! @16:34:40.69 AFV.select :holdingCoil @16:34:40.71 ATV.dial :air2 @16:34:41.37 AS.reconfigure ASslow1 @16:34:41.43 AS.seek 0.069ml @16:34:44.34
PSV.dial :waste1 @16:34:44.99 PS.seek 0ml @16:34:55.86 delay 3 seconds @16:34:58.89 ATV.dial :AIV @16:34:59.79 AS.seek 0.469ml @16:35:01.86
PSV.dial PSR @16:35:02.37 PSR.select :air @16:35:02.50 PS.seek 2.5ml @16:35:30.14 PRV.dial CBV @16:35:30.17
CBV.dial PRV @16:35:30.19 PSV.dial :mixloop @16:35:31.50 CTV.dial :air,avoiding: :intake @16:35:31.52 PTV.dial :PRVmixing @16:35:31.54 PS.seek 0ml @16:35:58.86 PSV.dial PSR @16:36:00.12 Pulling 1.5ml of PSR.flush @16:36:00.13 PSR.select :flush @16:36:00.26 PS.seek 1.5ml @16:36:16.86 delay 5 seconds @16:36:20.50 delay 5 seconds @16:36:21.89
PSV.dial :waste1 @16:36:22.54 PS.seek 0ml @16:36:25.58 Solid Phase Extraction Heater at 49.2C @16:36:25.59 ATV.dial ASV @16:36:25.99 ASV.dial :SPEdelivery @16:36:26.08 AS.seek 0.041ml @16:36:39.48
PSV.dial PSR @16:36:39.98 PSR.select :air @16:36:40.10 PS.seek 2.5ml @16:37:07.05 PRV.dial CBV @16:37:07.08
CBV.dial PRV @16:37:07.10 PSV.dial :mixloop @16:37:08.26 CTV.dial :air,avoiding: :intake @16:37:08.27 PTV.dial :PRVmixing @16:37:08.30 PS.seek 0ml @16:37:35.17 PSV.dial PSR @16:37:36.44 Pulling 1.5ml of PSR.flush @16:37:36.45 PSR.select :flush @16:37:36.59 PS.seek 1.5ml @16:37:52.43 delay 10 seconds @16:37:53.01
delay 5 seconds @16:37:58.04 PSV.dial :waste1 @16:37:58.56 PS.seek 0ml @16:38:02.45 rinseLysateLineINT @16:38:02.46 AFV.select :carrierSolution @16:38:02.57 delay 2 seconds @16:38:04.60 AS.reconfigure ASconfig @16:38:04.65 AS.seek 1ml @16:38:14.92
PSV.dial PSR @16:38:15.45 PSR.select :air @16:38:15.57 PS.seek 2.5ml @16:38:42.39 PRV.dial CBV @16:38:42.42
CBV.dial PRV @16:38:42.44 PSV.dial :mixloop @16:38:43.59 CTV.dial :air,avoiding: :intake @16:38:43.60 PTV.dial :PRVmixing @16:38:43.63 PS.reconfigure PSslow @16:38:43.67 PS.seek 1.2ml @16:39:00.05 delay 1 second @16:39:01.07 AFV.select :holdingCoil @16:39:01.17 delay 1 second @16:39:02.20 ATV.dial AIV @16:39:03.27 AIV.dial :coilToMFB @16:39:03.29 AWV.select :waste @16:39:03.41 AS.reconfigure ASslow0 @16:39:03.46 AS.seek 0ml @16:39:10.48
PSV.dial :waste1 @16:39:11.98 PS.reconfigure PSconfig @16:39:12.03 PS.seek 0ml @16:39:25.20 PSV.dial :air @16:39:26.37 PS.seek 2.5ml @16:39:52.83 PSV.dial :mixloop @16:39:53.34 PS.reconfigure PSslow @16:39:53.39 PS.seek 0ml @16:40:44.36 ATV.dial :air2 @16:40:44.52
PSV.dial :air @16:40:45.07 AS.reconfigure ASslow1 @16:40:45.10
PS.reconfigure PSconfig @16:40:45.21 AS.seek 0.05ml @16:40:45.24
PS.seek 2.5ml @16:40:55.68 ATV.dial :AIV @16:40:56.53 AS.seek 0.01ml @16:41:04.89 ATV.dial :waste @16:41:06.55 AS.seek :empty @16:41:08.96 ethanol wash @16:41:08.97 ATV.dial :air2 @16:41:10.26 AS.reconfigure ASslow2 @16:41:10.31 AS.seek 0.009ml @16:41:11.73
PSV.dial :mixloop @16:41:12.24 PS.reconfigure PSslow @16:41:12.29 PS.seek 0ml @16:41:20.69 delay 3 seconds @16:41:23.72 Pulling 0.4ml of Ethanol Wash @16:41:23.73 ATV.dial :ethanol @16:41:24.56 AS.reconfigure ASslow1 @16:41:24.61 AS.seek 0.409ml @16:42:03.61
Cleaning Collection Syringe @16:42:03.62 CSV.dial CSR @16:42:04.19 Pulling 4ml of CSR.flush CSR.select :flush @16:42:04.33 CS.seek 4ml @16:42:15.62 delay 5 seconds @16:42:20.65 CSR.select :air @16:42:20.78 CS.seek 10ml @16:42:37.03 CSV.dial CTV @16:42:37.07
CTV.dial CSV @16:42:38.64 CBV.dial :waste1 @16:42:39.18 CS.seek 0ml @16:42:45.32 delay 3 seconds @16:42:48.35 ATV.dial :air1 @16:42:48.75 delay 3 seconds @16:42:51.84 AS.seek 0.434ml @16:42:57.15 delay 3 seconds @16:43:00.18 ATV.dial :ethanol @16:43:00.81 AS.seek 0.419ml @16:43:04.10 delay 3 seconds @16:43:05.69
CSV.dial CSR @16:43:06.27 Pulling 4ml of CSR.flush @16:43:06.28 CSR.select :flush @16:43:06.40 CS.seek 4ml @16:43:07.13 ATV.dial ASV @16:43:08.48 AS.seek 0.092ml @16:43:17.75
delay 5 seconds @16:43:22.78 CSR.select :air @16:43:22.91 CS.seek 10ml @16:43:39.22 CSV.dial CTV @16:43:39.26
CTV.dial CSV @16:43:39.76 CBV.dial :waste1 @16:43:39.78 CS.seek 0ml @16:44:06.38 Garaging FlushPuck in Collection Clamp @16:44:06.46 Elbow.reconfigure TightElbow @16:44:06.51 Elbow.seek CC @16:44:06.79 Elbow.reconfigure LooseElbow @16:44:06.90 Forearm.seek CC @16:44:07.91 Hand.close @16:44:09.18 CC.seek :open @16:44:13.58 Forearm.seek :retract @16:44:14.58 Elbow.reconfigure TightElbow @16:44:14.64 Elbow.seek :garage @16:44:14.71 delay 3 seconds @16:44:17.68
Elbow.reconfigure LooseElbow @16:44:17.79 Forearm.reconfigure TightForearm @16:44:17.85 ATV.dial :waste2 @16:44:17.88
Forearm.seek :garage @16:44:18.68 AS.reconfigure ASconfig @16:44:18.73 AS.seek :empty @16:44:19.10
Hand.open @16:44:19.88 Forearm.reconfigure LooseForearm @16:44:19.93 Forearm.seek :retract @16:44:21.00 Whole Cell Archive sampling 1200ml @16:44:21.02 Loading wcr puck from tube 2 into Collection Clamp @16:44:21.29 SC.reconfigure SCtight @16:44:21.34 SC.seek 2 @16:44:23.51 SC.reconfigure SCloose @16:44:23.76 Elbow.reconfigure TightElbow @16:44:23.82 Elbow.seek 2 @16:44:24.39 ATV.dial :air2 @16:44:24.78 AS.reconfigure ASslow1 @16:44:24.83 AS.seek 1ml @16:44:25.85 Forearm.reconfigure TightForearm @16:44:25.91 Forearm.seek 2 + 700 counts @16:44:26.99 SE.reconfigure SEpick @16:44:27.07 SE.seek beamBreak @16:44:28.30 SE.configure SEconfig @16:44:28.48 Elbow.seek 2 @16:44:28.70 Forearm.seek 2 + 300 counts @16:44:28.91 Forearm.reconfigure LooseForearm @16:44:28.96 Hand.close @16:44:29.69 SE.seek :down @16:44:29.92 Forearm.seek :retract @16:44:30.73 SE.coast @16:44:30.93 Elbow.seek CC @16:44:32.59 Elbow.reconfigure LooseElbow @16:44:32.70 Forearm.reconfigure TightForearm @16:44:32.76 Forearm.seek CC @16:44:34.12 CC.seek :closed @16:44:38.42 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.55 @16:44:38.43 Hand.open @16:44:39.25 Forearm.reconfigure LooseForearm @16:44:39.82 Forearm.seek :retract @16:44:40.83 SC.coast @16:44:40.87 collectSample 1200ml w/bubble point of 55psi @16:44:40.89 Priming 7ml @16:44:40.90 Intake.select :close @16:44:40.91 Exhaust.select :close @16:44:40.93 SS.hold @16:44:41.16 Calibrating pressure sensors @16:44:41.18 CTV.dial :air @16:44:42.69 SSV.dial :spit,avoiding: :exhaust @16:44:42.72 SS.reconfigure SSfast @16:44:42.77 SS.seek 5ml @16:44:45.81 SSV.dial CBV @16:44:45.84 CBV.dial SSV @16:44:47.43 delay 3.8 seconds @16:44:51.39 SSV.dial :spit @16:44:52.89 SS.seek 0ml @16:44:55.50 SSV.dial CBV @16:44:57.00 CTV.dial :intake @16:44:57.85 SSV.dial CBV @16:44:57.88 CBV.dial SSV @16:44:57.89 Allowing 40s for 7.0ml of remaining 7.0ml @16:44:57.90 Intake.select :open @16:45:00.09 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:45:00.11 SS.reconfigure SSinhale.with maxSpeed: 150 @16:45:00.16 SS.seek 7ml @16:45:06.09 Stroke completed -- waiting for pressure to equalize ... @16:45:24.57 Intake.select :close @16:45:24.73 SSV.dial :exhaust @16:45:25.61 Exhaust.select :open @16:45:25.73 SS.reconfigure SSexhale @16:45:25.78 SS.seek 0ml @16:45:29.86 Exhaust.select :close @16:45:29.97 Sampling 1200.0ml @16:45:29.99 Intake.select :close @16:45:30.00 Exhaust.select :close @16:45:30.01 SS.reconfigure SSconfig @16:45:30.06 SS.hold @16:45:30.29 CTV.dial :intake @16:45:30.85 SSV.dial CBV @16:45:30.88 CBV.dial SSV @16:45:31.75 Allowing 130s for 25.0ml of remaining 1200.0ml @16:45:31.76 Intake.select :open @16:45:33.95 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:45:33.97 SS.reconfigure SSinhale.with maxSpeed: 150 @16:45:34.02 SS.seek 25ml @16:45:39.23 Vacuum too high -- waiting up to 40s @16:45:50.87 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:45:50.89 SS.reconfigure SSinhale.with maxSpeed: 135 @16:45:50.94 SS.seek 25ml @16:45:54.70 Vacuum too high -- waiting up to 40s @16:46:07.65 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:46:07.67 SS.reconfigure SSinhale.with maxSpeed: 122 @16:46:07.72 SS.seek 25ml @16:46:11.91 Vacuum too high -- waiting up to 40s @16:46:24.97 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:46:24.99 SS.reconfigure SSinhale.with maxSpeed: 110 @16:46:25.04 SS.seek 25ml @16:46:29.47 Vacuum too high -- waiting up to 40s @16:46:42.23 SS.reconfigure SSinhale.with maxSpeed: 99 @16:46:42.28 SS.seek 25ml @16:46:44.33 Stroke completed -- waiting for pressure to equalize ... @16:46:52.67 Intake.select :close @16:46:52.83 SSV.dial :exhaust @16:46:53.70 Exhaust.select :open @16:46:53.82 SS.reconfigure SSexhale @16:46:53.87 SS.seek 0ml @16:47:03.69 Exhaust.select :close @16:47:03.81 SSV.dial CBV @16:47:03.84 CBV.dial SSV @16:47:04.72 Allowing 130s for 25.0ml of remaining 1175.0ml @16:47:04.74 Intake.select :open @16:47:06.91 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:47:06.93 SS.reconfigure SSinhale.with maxSpeed: 99 @16:47:06.99 SS.seek 25ml @16:47:10.32 Vacuum too high -- waiting up to 40s @16:47:16.37 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:47:16.39 SS.reconfigure SSinhale.with maxSpeed: 89 @16:47:16.44 SS.seek 25ml @16:47:19.22 Vacuum too high -- waiting up to 40s @16:47:25.48 SS.reconfigure SSinhale.with maxSpeed: 80 @16:47:25.53 SS.seek 25ml @16:47:29.13 Vacuum too high -- waiting up to 40s @16:47:36.46 SS.reconfigure SSinhale.with maxSpeed: 72 @16:47:36.51 SS.seek 25ml @16:47:40.51 Vacuum too high -- waiting up to 40s @16:47:46.51 delay 5 seconds @16:47:47.88 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:47:47.90 SS.reconfigure SSinhale.with maxSpeed: 65 @16:47:47.95 SS.seek 25ml @16:47:51.55 ATV.dial ASV @16:47:52.57 ASV.dial :SPEdelivery @16:47:52.60 AS.seek 0.02ml @16:47:52.70 Vacuum too high -- waiting up to 40s @16:48:00.57 SS.reconfigure SSinhale.with maxSpeed: 59 @16:48:00.63 SS.seek 25ml @16:48:06.51 Vacuum too high -- waiting up to 40s @16:48:15.30 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:48:15.32 SS.reconfigure SSinhale.with maxSpeed: 53 @16:48:15.37 SS.seek 25ml @16:48:19.28 Stroke completed -- waiting for pressure to equalize ... @16:48:27.52 Intake.select :close @16:48:27.68 SSV.dial :exhaust @16:48:28.56 Exhaust.select :open @16:48:28.68 SS.reconfigure SSexhale @16:48:28.73 SS.seek 0ml @16:48:38.27 Exhaust.select :close @16:48:38.39 SSV.dial CBV @16:48:38.42 CBV.dial SSV @16:48:39.28 Allowing 130s for 25.0ml of remaining 1150.0ml @16:48:39.30 Intake.select :open @16:48:41.48 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:48:41.50 SS.reconfigure SSinhale.with maxSpeed: 53 @16:48:41.55 SS.seek 25ml @16:48:48.07 Vacuum too high -- waiting up to 40s @16:48:54.35 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:48:54.37 SS.reconfigure SSinhale.with maxSpeed: 48 @16:48:54.42 SS.seek 25ml @16:49:00.82 Vacuum too high -- waiting up to 40s @16:49:07.89 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:49:07.91 SS.reconfigure SSinhale.with maxSpeed: 43 @16:49:07.96 SS.seek 25ml @16:49:16.54 Vacuum too high -- waiting up to 40s @16:49:24.56 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:49:24.58 SS.reconfigure SSinhale.with maxSpeed: 39 @16:49:24.63 SS.seek 25ml @16:49:35.69 Vacuum too high -- waiting up to 40s @16:49:44.06 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:49:44.08 SS.reconfigure SSinhale.with maxSpeed: 35 @16:49:44.13 SS.seek 25ml @16:49:55.00 Stroke completed -- waiting for pressure to equalize ... @16:50:07.12 Intake.select :close @16:50:07.28 SSV.dial :exhaust @16:50:08.14 Exhaust.select :open @16:50:08.26 SS.reconfigure SSexhale @16:50:08.31 SS.seek 0ml @16:50:17.86 Exhaust.select :close @16:50:17.98 SSV.dial CBV @16:50:18.01 CBV.dial SSV @16:50:18.87 Allowing 130s for 25.0ml of remaining 1125.0ml @16:50:18.89 Intake.select :open @16:50:21.07 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:50:21.09 SS.reconfigure SSinhale.with maxSpeed: 35 @16:50:21.14 SS.seek 25ml @16:50:35.19 Vacuum too high -- waiting up to 40s @16:50:41.74 Intake @15.4psia, vacuum from 8.9 to 12.9psi @16:50:41.76 SS.reconfigure SSinhale.with maxSpeed: 32 @16:50:41.81 SS.seek 25ml @16:50:57.97 Vacuum too high -- waiting up to 40s @16:51:06.02 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:51:06.04 SS.reconfigure SSinhale.with maxSpeed: 29 @16:51:06.09 SS.seek 25ml @16:51:09.96 delay 5 seconds @16:51:14.99 ATV.dial :air2 @16:51:16.07 AS.seek 0.52ml @16:51:32.80 Stroke completed -- waiting for pressure to equalize ... @16:51:47.36 Intake.select :close @16:51:47.51 SSV.dial :exhaust @16:51:48.39 Exhaust.select :open @16:51:48.51 SS.reconfigure SSexhale @16:51:48.56 SS.seek 0ml @16:51:57.87 Exhaust.select :close @16:51:57.99 SSV.dial CBV @16:51:58.02 CBV.dial SSV @16:51:58.87 Allowing 130s for 25.0ml of remaining 1100.0ml @16:51:58.89 Intake.select :open @16:52:01.07 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:52:01.09 SS.reconfigure SSinhale.with maxSpeed: 29 @16:52:01.13 SS.seek 25ml @16:52:23.79 Vacuum too high -- waiting up to 40s @16:52:30.85 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:52:30.87 SS.reconfigure SSinhale.with maxSpeed: 26 @16:52:30.92 SS.seek 25ml @16:52:56.92 delay 5 seconds @16:53:01.95 ATV.dial ASV @16:53:02.96 ASV.dial :wasteR @16:53:03.36 AS.seek 0.3ml @16:53:14.32 Vacuum too high -- waiting up to 40s @16:53:23.48 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:53:23.50 SS.reconfigure SSinhale.with maxSpeed: 23 @16:53:23.55 SS.seek 25ml @16:53:24.09 Stroke completed -- waiting for pressure to equalize ... @16:53:29.24 Intake.select :close @16:53:29.40 SSV.dial :exhaust @16:53:30.28 Exhaust.select :open @16:53:30.40 SS.reconfigure SSexhale @16:53:30.45 SS.seek 0ml @16:53:39.72 Exhaust.select :close @16:53:39.84 SSV.dial CBV @16:53:39.87 CBV.dial SSV @16:53:40.75 Allowing 130s for 25.0ml of remaining 1075.0ml @16:53:40.77 Intake.select :open @16:53:42.94 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:53:42.96 SS.reconfigure SSinhale.with maxSpeed: 23 @16:53:43.02 SS.seek 25ml @16:53:47.91 delay 20 seconds @16:54:07.95 AS.seek 0.07ml @16:54:54.57 delay 20 seconds @16:55:00.58 Stroke completed -- waiting for pressure to equalize ... @16:55:13.24 Intake.select :close @16:55:13.40 SSV.dial :exhaust @16:55:14.28 Exhaust.select :open @16:55:14.41 SS.reconfigure SSexhale @16:55:14.46 SS.seek 0ml @16:55:14.59 ATV.dial :waste2 @16:55:15.36 AS.reconfigure ASconfig @16:55:15.40 AS.seek :empty @16:55:19.78 Elution @16:55:19.79 ATV.dial :air1 @16:55:20.93 AS.reconfigure ASslow1 @16:55:20.99 AS.seek 0.049ml @16:55:24.08 Exhaust.select :close @16:55:24.20 SSV.dial CBV @16:55:24.23 CBV.dial SSV @16:55:25.12 Allowing 130s for 25.0ml of remaining 1050.0ml @16:55:25.14 Intake.select :open @16:55:27.32 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:55:27.34 SS.reconfigure SSinhale.with maxSpeed: 23 @16:55:27.39 SS.seek 25ml @16:55:31.36 delay 3 seconds @16:55:34.39 ATV.dial :ElutionWater @16:55:35.07 AS.seek 0.109ml @16:55:47.39 delay 3 seconds @16:55:50.41 ATV.dial ASV,via: :air2 @16:55:52.56 ASV.dial :SPEelution @16:55:53.17 AFV.select :carrierSolution @16:55:53.34 AS.reconfigure ASnorm @16:55:53.39 AS.seek 0.759ml @16:56:26.42 delay 3 seconds @16:56:29.45 AFV.select :holdingCoil @16:56:29.61 AS.reconfigure ASslow1 @16:56:29.66 AS.seek 0.474ml @16:56:44.59 Stroke completed -- waiting for pressure to equalize ... @16:56:57.96 Intake.select :close @16:56:58.12 SSV.dial :exhaust @16:56:58.98 Exhaust.select :open @16:56:59.55 SS.reconfigure SSexhale @16:56:59.70 SS.seek 0ml @16:57:08.97 Exhaust.select :close @16:57:09.09 SSV.dial CBV @16:57:09.14 CBV.dial SSV @16:57:10.00 Allowing 130s for 25.0ml of remaining 1025.0ml @16:57:10.02 Intake.select :open @16:57:12.20 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:57:12.22 SS.reconfigure SSinhale.with maxSpeed: 23 @16:57:12.27 SS.seek 25ml @16:57:27.30 delay 5 seconds @16:57:32.40 AS.reconfigure ASslow2 @16:57:32.45 AS.seek 0.439ml @16:58:07.97 delay 1:00 @16:58:30.11 Stroke completed -- waiting for pressure to equalize ... @16:58:44.72 Intake.select :close @16:58:44.88 SSV.dial :exhaust @16:58:45.73 Exhaust.select :open @16:58:45.85 SS.reconfigure SSexhale @16:58:45.90 SS.seek 0ml @16:58:55.64 Exhaust.select :close @16:58:55.76 SSV.dial CBV @16:58:55.79 CBV.dial SSV @16:58:56.65 Allowing 130s for 25.0ml of remaining 1000.0ml @16:58:56.67 Intake.select :open @16:58:58.85 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:58:58.87 SS.reconfigure SSinhale.with maxSpeed: 23 @16:58:58.92 SS.seek 25ml @16:59:08.00 pushing eluate to holding coil @16:59:08.08 AS.seek 0.279ml @17:00:16.40 Stroke completed -- waiting for pressure to equalize ... @17:00:31.00 Intake.select :close @17:00:31.17 SSV.dial :exhaust @17:00:32.04 Exhaust.select :open @17:00:32.16 SS.reconfigure SSexhale @17:00:32.21 SS.seek 0ml @17:00:41.85 Exhaust.select :close @17:00:41.97 SSV.dial CBV @17:00:42.00 CBV.dial SSV @17:00:42.86 Allowing 130s for 25.0ml of remaining 975.0ml @17:00:42.88 Intake.select :open @17:00:45.05 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:00:45.08 SS.reconfigure SSinhale.with maxSpeed: 23 @17:00:45.12 SS.seek 25ml @17:01:49.16 delay 30 seconds @17:02:02.42 Stroke completed -- waiting for pressure to equalize ... @17:02:17.67 Intake.select :close @17:02:17.83 SSV.dial :exhaust @17:02:18.68 Exhaust.select :open @17:02:18.80 SS.reconfigure SSexhale @17:02:18.85 SS.seek 0ml @17:02:19.18 ATV.dial :waste,via: :air3 @17:02:20.92 AS.reconfigure ASconfig @17:02:20.96 AS.seek :empty @17:02:28.51 Exhaust.select :close @17:02:28.63 SSV.dial CBV @17:02:28.66 CBV.dial SSV @17:02:29.54 Allowing 130s for 25.0ml of remaining 950.0ml @17:02:29.55 Intake.select :open @17:02:31.74 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:02:31.76 SS.reconfigure SSinhale.with maxSpeed: 23 @17:02:31.81 SS.seek 25ml @17:02:37.36 Parking Bleach in LysateSource line and SPEcolumn @17:02:37.37 ATV.dial :air3 @17:02:38.21 AS.reconfigure ASslow1 @17:02:38.26 AS.seek 0.029ml @17:02:44.60 ATV.dial :ElutionWater @17:02:45.21 AS.seek 0.059ml @17:02:51.50 ATV.dial :air3 @17:02:52.12 AS.seek 0.069ml @17:02:54.94 delay 2 seconds @17:02:56.96 ATV.dial :bleach @17:02:57.44 AS.seek 0.769ml @17:03:49.09 Stroke completed -- waiting for pressure to equalize ... @17:04:05.63 Intake.select :close @17:04:05.79 SSV.dial :exhaust @17:04:06.67 Exhaust.select :open @17:04:06.79 SS.reconfigure SSexhale @17:04:06.84 SS.seek 0ml @17:04:16.59 Exhaust.select :close @17:04:16.71 SSV.dial CBV @17:04:16.74 CBV.dial SSV @17:04:17.59 Allowing 130s for 25.0ml of remaining 925.0ml @17:04:17.61 Intake.select :open @17:04:19.78 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:04:19.80 SS.reconfigure SSinhale.with maxSpeed: 23 @17:04:19.85 SS.seek 25ml @17:05:18.47 delay 3 seconds @17:05:21.50 ATV.dial :air3 @17:05:22.06 delay 3 seconds @17:05:25.15 AS.seek 0.794ml @17:05:30.61 delay 3 seconds @17:05:33.64 ATV.dial :bleach @17:05:34.28 AS.seek 0.779ml @17:05:37.60 delay 3 seconds @17:05:37.64 Stroke completed -- waiting for pressure to equalize ... @17:05:40.63 ATV.dial :AIV @17:05:42.05 AS.reconfigure ASconfig @17:05:42.13 AS.seek 0.015ml @17:05:53.72 Intake.select :close @17:05:53.88 SSV.dial :exhaust @17:05:54.74 Exhaust.select :open @17:05:54.86 SS.reconfigure SSexhale @17:05:54.91 SS.seek 0ml @17:06:04.21 Exhaust.select :close @17:06:04.33 SSV.dial CBV @17:06:04.36 CBV.dial SSV @17:06:05.23 Allowing 130s for 25.0ml of remaining 900.0ml @17:06:05.25 Intake.select :open @17:06:07.42 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:06:07.45 SS.reconfigure SSinhale.with maxSpeed: 23 @17:06:07.49 SS.seek 25ml @17:06:26.41 ATV.dial :waste @17:06:27.94 AS.seek :empty @17:06:29.13 ATV.dial :air3 @17:06:29.82 AS.reconfigure ASslow1 @17:06:29.87 AS.seek 0.009ml @17:06:32.17 delay 2 seconds @17:06:34.19 ATV.dial :bleach @17:06:34.65 AS.seek 0.579ml @17:07:24.88 Stroke completed -- waiting for pressure to equalize ... @17:07:42.81 Intake.select :close @17:07:42.98 SSV.dial :exhaust @17:07:43.84 Exhaust.select :open @17:07:44.52 SS.reconfigure SSexhale @17:07:44.57 SS.seek 0ml @17:07:53.85 Exhaust.select :close @17:07:53.97 SSV.dial CBV @17:07:54.00 CBV.dial SSV @17:07:54.87 Allowing 130s for 25.0ml of remaining 875.0ml @17:07:54.89 Intake.select :open @17:07:57.07 Intake @15.9psia, vacuum from 9.4 to 13.4psi @17:07:57.09 SS.reconfigure SSinhale.with maxSpeed: 23 @17:07:57.14 SS.seek 25ml @17:08:29.53 delay 3 seconds @17:08:32.56 ATV.dial :air3 @17:08:33.13 delay 3 seconds @17:08:36.23 AS.seek 0.604ml @17:08:41.56 delay 3 seconds @17:08:44.59 ATV.dial :bleach @17:08:45.24 AS.seek 0.589ml @17:08:48.67 delay 3 seconds @17:08:51.70 ATV.dial :ASV @17:08:52.74 ASV.dial :wasteR @17:08:53.36 AS.seek 0ml @17:09:03.75 Vacuum too high -- waiting up to 40s @17:09:14.18 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:09:14.20 SS.reconfigure SSinhale.with maxSpeed: 21 @17:09:14.25 SS.seek 25ml @17:09:26.77 Stroke completed -- waiting for pressure to equalize ... @17:09:35.60 Intake.select :close @17:09:35.76 SSV.dial :exhaust @17:09:36.63 Exhaust.select :open @17:09:36.75 SS.reconfigure SSexhale @17:09:36.80 SS.seek 0ml @17:09:46.57 Exhaust.select :close @17:09:46.69 SSV.dial CBV @17:09:46.75 CBV.dial SSV @17:09:47.57 Allowing 130s for 25.0ml of remaining 850.0ml @17:09:47.59 Intake.select :open @17:09:49.76 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:09:49.78 SS.reconfigure SSinhale.with maxSpeed: 21 @17:09:49.84 SS.seek 25ml @17:10:52.16 delay 1:00 @17:11:15.34 Stroke completed -- waiting for pressure to equalize ... @17:11:29.38 Intake.select :close @17:11:29.54 SSV.dial :exhaust @17:11:30.42 Exhaust.select :open @17:11:30.54 SS.reconfigure SSexhale @17:11:30.59 SS.seek 0ml @17:11:40.29 Exhaust.select :close @17:11:40.41 SSV.dial CBV @17:11:40.44 CBV.dial SSV @17:11:41.30 Allowing 130s for 25.0ml of remaining 825.0ml @17:11:41.32 Intake.select :open @17:11:43.50 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:11:43.52 SS.reconfigure SSinhale.with maxSpeed: 21 @17:11:43.57 SS.seek 25ml @17:11:52.19 AFV.select :carrierSolution @17:11:52.29 delay 2 seconds @17:11:54.32 AS.reconfigure ASconfig @17:11:54.37 AS.seek 1ml @17:12:52.13 delay 1 second @17:12:53.15 AFV.select :holdingCoil @17:12:53.25 delay 1 second @17:12:54.28 ATV.dial ASV @17:12:54.29 ASV.dial :wasteR @17:12:54.31 AS.reconfigure ASslow1 @17:12:54.36 AS.seek 0ml @17:13:08.38 Stroke completed -- waiting for pressure to equalize ... @17:13:22.88 Intake.select :close @17:13:23.04 SSV.dial :exhaust @17:13:23.91 Exhaust.select :open @17:13:24.04 SS.reconfigure SSexhale @17:13:24.09 SS.seek 0ml @17:13:33.79 Exhaust.select :close @17:13:33.91 SSV.dial CBV @17:13:33.94 CBV.dial SSV @17:13:34.80 Allowing 130s for 25.0ml of remaining 800.0ml @17:13:34.82 Intake.select :open @17:13:37.00 Intake @15.9psia, vacuum from 9.4 to 13.4psi @17:13:37.02 SS.reconfigure SSinhale.with maxSpeed: 21 @17:13:37.07 SS.seek 25ml @17:15:01.96 Stroke completed -- waiting for pressure to equalize ... @17:15:17.23 Intake.select :close @17:15:17.39 SSV.dial :exhaust @17:15:18.24 Exhaust.select :open @17:15:18.36 SS.reconfigure SSexhale @17:15:18.97 SS.seek 0ml @17:15:28.66 Exhaust.select :close @17:15:28.78 SSV.dial CBV @17:15:28.87 CBV.dial SSV @17:15:29.68 Allowing 130s for 25.0ml of remaining 775.0ml @17:15:29.70 Intake.select :open @17:15:31.88 Intake @15.9psia, vacuum from 9.4 to 13.4psi @17:15:31.90 SS.reconfigure SSinhale.with maxSpeed: 21 @17:15:31.95 SS.seek 25ml @17:16:15.77 delay 20 seconds @17:16:35.80 ATV.dial :air3 @17:16:37.36 AS.seek 0.59ml @17:16:56.66 Stroke completed -- waiting for pressure to equalize ... @17:17:11.95 Intake.select :close @17:17:12.11 SSV.dial :exhaust @17:17:12.99 Exhaust.select :open @17:17:13.11 SS.reconfigure SSexhale @17:17:13.16 SS.seek 0ml @17:17:22.82 Exhaust.select :close @17:17:22.94 SSV.dial CBV @17:17:22.97 CBV.dial SSV @17:17:23.83 Allowing 130s for 25.0ml of remaining 750.0ml @17:17:23.85 Intake.select :open @17:17:26.03 Intake @16.0psia, vacuum from 9.5 to 13.5psi @17:17:26.05 SS.reconfigure SSinhale.with maxSpeed: 21 @17:17:26.10 SS.seek 25ml @17:18:36.31 delay 3 seconds @17:18:39.34 ATV.dial :ASV @17:18:40.78 ASV.dial :wasteR @17:18:40.87 AS.seek 0.09ml @17:18:51.02 Stroke completed -- waiting for pressure to equalize ... @17:19:06.27 Intake.select :close @17:19:06.43 SSV.dial :exhaust @17:19:07.30 Exhaust.select :open @17:19:07.42 SS.reconfigure SSexhale @17:19:07.47 SS.seek 0ml @17:19:17.11 Exhaust.select :close @17:19:17.23 SSV.dial CBV @17:19:17.26 CBV.dial SSV @17:19:18.11 Allowing 130s for 25.0ml of remaining 725.0ml @17:19:18.13 Intake.select :open @17:19:20.31 Intake @15.9psia, vacuum from 9.4 to 13.4psi @17:19:20.33 SS.reconfigure SSinhale.with maxSpeed: 21 @17:19:20.38 SS.seek 25ml @17:20:21.72 delay 30 seconds @17:20:45.38 Stroke completed -- waiting for pressure to equalize ... @17:20:51.75 ATV.dial :extSrc @17:20:52.55 delay 3 seconds @17:20:55.57 ATV.dial :ElutionWater @17:20:56.71 AS.seek 0.12ml @17:21:01.39 Intake.select :close @17:21:01.55 SSV.dial :exhaust @17:21:02.41 Exhaust.select :open @17:21:02.53 SS.reconfigure SSexhale @17:21:02.58 SS.seek 0ml @17:21:03.13 ATV.dial :air3 @17:21:03.76 AS.seek 0.13ml @17:21:06.06 delay 2 seconds @17:21:08.09 ATV.dial :bleach @17:21:08.56 AS.seek 0.23ml @17:21:12.35 Exhaust.select :close @17:21:12.47 SSV.dial CBV @17:21:12.50 CBV.dial SSV @17:21:13.93 Allowing 130s for 25.0ml of remaining 700.0ml @17:21:13.95 Intake.select :open @17:21:16.13 Intake @16.0psia, vacuum from 9.5 to 13.5psi @17:21:16.15 SS.reconfigure SSinhale.with maxSpeed: 21 @17:21:16.20 SS.seek 25ml @17:21:28.97 delay 3 seconds @17:21:32.00 ATV.dial :air3 @17:21:32.56 delay 3 seconds @17:21:35.65 AS.seek 0.255ml @17:21:41.08 delay 3 seconds @17:21:44.11 ATV.dial :bleach @17:21:44.75 AS.seek 0.24ml @17:21:48.16 delay 3 seconds @17:21:51.19 ATV.dial :ASV @17:21:52.24 ASV.dial :SPEdelivery @17:21:52.72 AFV.select :carrierSolution @17:21:52.83 delay 2 seconds @17:21:54.87 AS.reconfigure ASconfig @17:21:54.91 AS.seek 0.5ml @17:22:10.14 delay 1 second @17:22:11.16 AFV.select :holdingCoil @17:22:11.32 AS.reconfigure ASslow1 @17:22:11.37 AS.seek 0.17ml @17:22:41.64 Stroke completed -- waiting for pressure to equalize ... @17:22:56.91 Intake.select :close @17:22:57.07 SSV.dial :exhaust @17:22:57.93 Exhaust.select :open @17:22:58.05 SS.reconfigure SSexhale @17:22:58.10 SS.seek 0ml @17:23:07.83 Exhaust.select :close @17:23:07.96 SSV.dial CBV @17:23:07.99 CBV.dial SSV @17:23:08.85 Allowing 130s for 25.0ml of remaining 675.0ml @17:23:08.87 Intake.select :open @17:23:11.05 Intake @16.0psia, vacuum from 9.5 to 13.5psi @17:23:11.07 SS.reconfigure SSinhale.with maxSpeed: 21 @17:23:11.12 SS.seek 25ml @17:23:18.03 SPE.hold 2:46:40,95C @17:23:18.08 delay 3:00 @17:24:36.03 Stroke completed -- waiting for pressure to equalize ... @17:24:51.92 Intake.select :close @17:24:52.08 SSV.dial :exhaust @17:24:52.97 Exhaust.select :open @17:24:53.09 SS.reconfigure SSexhale @17:24:53.14 SS.seek 0ml @17:25:02.96 Exhaust.select :close @17:25:03.08 SSV.dial CBV @17:25:03.11 CBV.dial SSV @17:25:04.00 Allowing 130s for 25.0ml of remaining 650.0ml @17:25:04.02 Intake.select :open @17:25:06.20 Intake @16.0psia, vacuum from 9.5 to 13.5psi @17:25:06.22 SS.reconfigure SSinhale.with maxSpeed: 21 @17:25:06.27 SS.seek 25ml @17:26:18.11 AFV.select :carrierSolution @17:26:18.21 delay 2 seconds @17:26:20.24 AS.reconfigure ASconfig @17:26:20.29 AS.seek 1ml @17:26:31.00 Stroke completed -- waiting for pressure to equalize ... @17:26:47.53 Intake.select :close @17:26:47.68 SSV.dial :exhaust @17:26:48.58 Exhaust.select :open @17:26:48.70 SS.reconfigure SSexhale @17:26:48.75 SS.seek 0ml @17:26:58.60 Exhaust.select :close @17:26:58.72 SSV.dial CBV @17:26:58.75 CBV.dial SSV @17:26:59.66 Allowing 130s for 25.0ml of remaining 625.0ml @17:27:00.23 Intake.select :open @17:27:02.41 Intake @16.0psia, vacuum from 9.5 to 13.5psi @17:27:02.43 SS.reconfigure SSinhale.with maxSpeed: 21 @17:27:02.48 SS.seek 25ml @17:27:08.30 delay 1 second @17:27:09.33 AFV.select :holdingCoil @17:27:09.43 delay 1 second @17:27:10.46 ASV.dial :wasteD @17:27:11.57 AS.seek 0ml @17:28:09.34 Priming Eluate @17:28:09.35 AFV.select :carrierSolution @17:28:09.46 delay 2 seconds @17:28:11.50 AS.seek 0.5ml @17:28:27.66 Stroke completed -- waiting for pressure to equalize ... @17:28:40.53 delay 1 second @17:28:41.61 AFV.select :holdingCoil @17:28:41.71 ATV.dial ASV @17:28:41.73 ASV.dial :SPErecovery @17:28:42.36 Swish #1 of 2 @17:28:42.44 AS.reconfigure ASslow0 @17:28:42.49 AS.seek 0.6ml @17:28:43.57 Intake.select :close @17:28:43.73 SSV.dial :exhaust @17:28:44.63 Exhaust.select :open @17:28:44.75 SS.reconfigure SSexhale @17:28:44.80 SS.seek 0ml @17:28:52.87 AS.seek 0.5ml @17:28:54.50 Exhaust.select :close @17:28:54.61 SSV.dial CBV @17:28:54.65 CBV.dial SSV @17:28:55.53 Allowing 130s for 25.0ml of remaining 600.0ml @17:28:55.55 Intake.select :open @17:28:57.73 Intake @16.1psia, vacuum from 9.6 to 13.6psi @17:28:57.75 SS.reconfigure SSinhale.with maxSpeed: 21 @17:28:57.80 SS.seek 25ml @17:29:03.26 Swish #2 of 2 @17:29:03.34 AS.seek 0.6ml @17:29:13.81 AS.seek 0.5ml @17:29:24.23 delay 3 seconds @17:29:27.32 AS.reconfigure ASslow1 @17:29:27.37 AS.seek 0.684ml @17:30:04.69 delay 3 seconds @17:30:07.72 Eluate primed @17:30:07.73 ATV.dial :waste2 @17:30:08.37 AS.reconfigure ASnorm @17:30:08.42 AS.seek 0ml @17:30:22.61 Stroke completed -- waiting for pressure to equalize ... @17:30:39.16 Intake.select :close @17:30:39.32 SSV.dial :exhaust @17:30:40.19 Exhaust.select :open @17:30:40.32 SS.reconfigure SSexhale @17:30:40.37 SS.seek 0ml @17:30:43.24 SPE.coast @17:30:43.33 ASV.dial :SPErecovery @17:30:43.36 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep1217h1500ml.pcr @17:30:43.38 Building PCR Slug from ASV with MM2 mix, PP4 primer @17:30:43.39 Priming with carrier @17:30:43.40 ATV.dial ARV @17:30:44.47 ARV.dial :carrier @17:30:44.85 AS.seek 0.5ml @17:30:49.67 Exhaust.select :close @17:30:49.79 SSV.dial CBV @17:30:49.82 CBV.dial SSV @17:30:50.67 Allowing 130s for 25.0ml of remaining 575.0ml @17:30:50.69 Intake.select :open @17:30:52.87 Intake @16.0psia, vacuum from 9.5 to 13.5psi @17:30:52.89 SS.reconfigure SSinhale.with maxSpeed: 21 @17:30:52.94 SS.seek 25ml @17:31:10.42 AFV.select :carrierSolution @17:31:10.53 delay 2 seconds @17:31:12.57 AS.reconfigure ASconfig @17:31:12.61 AS.seek 1ml @17:31:41.64 delay 1 second @17:31:42.67 AFV.select :holdingCoil @17:31:42.77 delay 1 second @17:31:43.80 ATV.dial :waste @17:31:45.34 AS.reconfigure ASnorm @17:31:45.39 AS.seek :empty @17:32:18.42 Stroke completed -- waiting for pressure to equalize ... @17:32:35.53 Intake.select :close @17:32:35.69 SSV.dial :exhaust @17:32:36.16 AFV.select :carrierSolution @17:32:36.27 delay 2 seconds @17:32:36.55 Exhaust.select :open @17:32:36.68 SS.reconfigure SSexhale @17:32:36.73 SS.seek 0ml @17:32:38.31 AS.reconfigure ASconfig @17:32:38.36 AS.seek 0.5ml @17:32:46.58 Exhaust.select :close @17:32:46.70 SSV.dial CBV @17:32:46.73 CBV.dial SSV @17:32:47.61 Allowing 130s for 25.0ml of remaining 550.0ml @17:32:47.63 Intake.select :open @17:32:49.80 Intake @16.0psia, vacuum from 9.5 to 13.5psi @17:32:49.83 SS.reconfigure SSinhale.with maxSpeed: 21 @17:32:49.87 SS.seek 25ml @17:33:07.45 delay 1 second @17:33:08.48 AFV.select :holdingCoil @17:33:08.58 Building Slug @17:33:08.60 ATV.dial :ARV @17:33:10.24 delay 2 seconds @17:33:12.26 ARV.dial :air1 @17:33:12.67 delay 2 seconds @17:33:14.77 AS.reconfigure ASslow2 @17:33:14.82 AS.seek 0.506ml @17:33:21.05 delay 2 seconds @17:33:23.08 ARV.dial :MM2 @17:33:24.66 delay 2 seconds @17:33:26.69 Adding MM2 @17:33:26.77 AS.seek 0.524ml @17:33:45.28 delay 25 seconds @17:34:10.31 ARV.dial :PP4 @17:34:10.71 Adding PP4 @17:34:10.72 delay 2 seconds @17:34:12.80 AS.seek 0.53ml @17:34:14.86 Stroke completed -- waiting for pressure to equalize ... @17:34:19.06 delay 10 seconds @17:34:29.08 ARV.dial :air3 @17:34:29.49 adding ARV air @17:34:29.51 delay 2 seconds @17:34:31.37 Intake.select :close @17:34:31.54 SSV.dial :exhaust @17:34:31.66 AS.seek 0.536ml @17:34:32.39 Exhaust.select :open @17:34:32.52 SS.reconfigure SSexhale @17:34:32.57 SS.seek 0ml @17:34:38.03 delay 5 seconds @17:34:42.36 Exhaust.select :close @17:34:42.48 SSV.dial CBV @17:34:42.51 CBV.dial SSV @17:34:43.06 ARV.dial :carrier @17:34:43.37 Allowing 130s for 25.0ml of remaining 525.0ml @17:34:43.39 Intake.select :open @17:34:44.38 delay 7 seconds @17:34:45.57 Intake @16.1psia, vacuum from 9.6 to 13.6psi @17:34:45.59 SS.reconfigure SSinhale.with maxSpeed: 21 @17:34:45.64 SS.seek 25ml @17:34:51.48 AS.seek 0.594ml @17:35:50.13 delay 5 seconds @17:35:55.16 ATV.dial :ASV @17:35:55.55 delay 2 seconds @17:35:57.64 AS.seek 0.6ml @17:36:03.92 delay 4 seconds @17:36:07.95 ATV.dial ARV @17:36:08.59 delay 2 seconds @17:36:10.22 Stroke completed -- waiting for pressure to equalize ... @17:36:10.62 ARV.dial :carrier @17:36:10.64 delay 2 seconds @17:36:12.83 AS.seek 0.65ml @17:36:26.93 Intake.select :close @17:36:27.09 SSV.dial :exhaust @17:36:27.95 Exhaust.select :open @17:36:28.07 SS.reconfigure SSexhale @17:36:28.12 SS.seek 0ml @17:36:37.86 Exhaust.select :close @17:36:37.98 SSV.dial CBV @17:36:38.01 CBV.dial SSV @17:36:38.87 Allowing 130s for 25.0ml of remaining 500.0ml @17:36:38.89 Intake.select :open @17:36:41.07 Intake @16.1psia, vacuum from 9.6 to 13.6psi @17:36:41.09 SS.reconfigure SSinhale.with maxSpeed: 21 @17:36:41.14 SS.seek 25ml @17:37:03.34 delay 4 seconds @17:37:07.37 ATV.dial PCR @17:37:08.57 ASV.dial :waste1 @17:37:09.36 ARV.dial :air1 @17:37:09.78 Positioning Slug by 0.324ml @17:37:09.87 AS.seek 0.326ml @17:38:05.80 Stroke completed -- waiting for pressure to equalize ... @17:38:22.99 Intake.select :close @17:38:23.14 SSV.dial :exhaust @17:38:24.00 Exhaust.select :open @17:38:24.12 SS.reconfigure SSexhale @17:38:24.17 SS.seek 0ml @17:38:33.90 Exhaust.select :close @17:38:34.02 SSV.dial CBV @17:38:34.05 CBV.dial SSV @17:38:34.90 Allowing 130s for 25.0ml of remaining 475.0ml @17:38:34.92 Intake.select :open @17:38:37.10 Intake @16.2psia, vacuum from 9.7 to 13.7psi @17:38:37.12 SS.reconfigure SSinhale.with maxSpeed: 21 @17:38:37.16 SS.seek 25ml @17:40:01.97 Stroke completed -- waiting for pressure to equalize ... @17:40:19.08 Intake.select :close @17:40:19.24 SSV.dial :exhaust @17:40:20.11 Exhaust.select :open @17:40:20.23 SS.reconfigure SSexhale @17:40:20.28 SS.seek 0ml @17:40:29.54 Exhaust.select :close @17:40:29.66 SSV.dial CBV @17:40:29.69 CBV.dial SSV @17:40:30.54 Allowing 130s for 25.0ml of remaining 450.0ml @17:40:30.56 Intake.select :open @17:40:32.74 Intake @16.2psia, vacuum from 9.7 to 13.7psi @17:40:32.76 SS.reconfigure SSinhale.with maxSpeed: 21 @17:40:32.80 SS.seek 25ml @17:41:58.48 Stroke completed -- waiting for pressure to equalize ... @17:42:16.31 Intake.select :close @17:42:16.47 SSV.dial :exhaust @17:42:17.33 Exhaust.select :open @17:42:17.45 SS.reconfigure SSexhale @17:42:17.50 SS.seek 0ml @17:42:27.25 Exhaust.select :close @17:42:27.37 SSV.dial CBV @17:42:27.40 CBV.dial SSV @17:42:28.28 Allowing 130s for 25.0ml of remaining 425.0ml @17:42:28.30 Intake.select :open @17:42:30.48 Intake @16.2psia, vacuum from 9.7 to 13.7psi @17:42:30.50 SS.reconfigure SSinhale.with maxSpeed: 21 @17:42:30.55 SS.seek 25ml @17:42:35.95 delay 5 seconds @17:42:40.98 ATV.dial ARV @17:42:41.86 ASV.dial :PCRlock @17:42:42.83 delay 5 seconds @17:42:47.86 Running Tt1 Profile60C300 thermocycling @17:42:47.88 PCR.program Profile60C300 @17:42:48.01 Profile60C300 should complete in < 1:45:21 @17:42:48.03 PCR.run 2:16:41.25 @17:43:55.15 Stroke completed -- waiting for pressure to equalize ... @17:44:12.39 Intake.select :close @17:44:12.55 SSV.dial :exhaust @17:44:13.40 Exhaust.select :open @17:44:13.52 SS.reconfigure SSexhale @17:44:13.57 SS.seek 0ml @17:44:23.28 Exhaust.select :close @17:44:23.40 SSV.dial CBV @17:44:23.43 CBV.dial SSV @17:44:24.31 Allowing 130s for 25.0ml of remaining 400.0ml @17:44:24.33 Intake.select :open @17:44:26.51 Intake @16.2psia, vacuum from 9.7 to 13.7psi @17:44:26.53 SS.reconfigure SSinhale.with maxSpeed: 21 @17:44:26.57 SS.seek 25ml @17:45:51.45 Stroke completed -- waiting for pressure to equalize ... @17:46:08.55 Intake.select :close @17:46:08.70 SSV.dial :exhaust @17:46:09.56 Exhaust.select :open @17:46:09.69 SS.reconfigure SSexhale @17:46:09.74 SS.seek 0ml @17:46:19.46 Exhaust.select :close @17:46:19.58 SSV.dial CBV @17:46:19.61 CBV.dial SSV @17:46:20.48 Allowing 130s for 25.0ml of remaining 375.0ml @17:46:20.50 Intake.select :open @17:46:22.68 Intake @16.2psia, vacuum from 9.7 to 13.7psi @17:46:22.70 SS.reconfigure SSinhale.with maxSpeed: 21 @17:46:22.76 SS.seek 25ml @17:47:47.60 Stroke completed -- waiting for pressure to equalize ... @17:48:05.35 Intake.select :close @17:48:05.52 SSV.dial :exhaust @17:48:06.37 Exhaust.select :open @17:48:06.50 SS.reconfigure SSexhale @17:48:06.56 SS.seek 0ml @17:48:16.39 Exhaust.select :close @17:48:16.51 SSV.dial CBV @17:48:16.56 CBV.dial SSV @17:48:17.39 Allowing 130s for 25.0ml of remaining 350.0ml @17:48:17.41 Intake.select :open @17:48:19.58 Intake @16.2psia, vacuum from 9.7 to 13.7psi @17:48:19.61 SS.reconfigure SSinhale.with maxSpeed: 21 @17:48:19.65 SS.seek 25ml @17:49:44.56 Stroke completed -- waiting for pressure to equalize ... @17:50:01.73 Intake.select :close @17:50:01.89 SSV.dial :exhaust @17:50:02.74 Exhaust.select :open @17:50:02.86 SS.reconfigure SSexhale @17:50:02.91 SS.seek 0ml @17:50:12.13 Exhaust.select :close @17:50:12.25 SSV.dial CBV @17:50:12.28 CBV.dial SSV @17:50:13.14 Allowing 130s for 25.0ml of remaining 325.0ml @17:50:13.16 Intake.select :open @17:50:15.34 Intake @16.3psia, vacuum from 9.8 to 13.8psi @17:50:15.36 SS.reconfigure SSinhale.with maxSpeed: 21 @17:50:15.41 SS.seek 25ml @17:51:40.90 Stroke completed -- waiting for pressure to equalize ... @17:51:59.32 Intake.select :close @17:51:59.48 SSV.dial :exhaust @17:52:00.34 Exhaust.select :open @17:52:00.46 SS.reconfigure SSexhale @17:52:00.51 SS.seek 0ml @17:52:10.18 Exhaust.select :close @17:52:10.30 SSV.dial CBV @17:52:10.33 CBV.dial SSV @17:52:11.20 Allowing 130s for 25.0ml of remaining 300.0ml @17:52:11.22 Intake.select :open @17:52:13.40 Intake @16.3psia, vacuum from 9.8 to 13.8psi @17:52:13.42 SS.reconfigure SSinhale.with maxSpeed: 21 @17:52:13.48 SS.seek 25ml @17:53:38.20 Stroke completed -- waiting for pressure to equalize ... @17:53:56.68 Intake.select :close @17:53:56.84 SSV.dial :exhaust @17:53:57.70 Exhaust.select :open @17:53:57.83 SS.reconfigure SSexhale @17:53:57.88 SS.seek 0ml @17:54:07.51 Exhaust.select :close @17:54:07.63 SSV.dial CBV @17:54:07.66 CBV.dial SSV @17:54:08.52 Allowing 130s for 25.0ml of remaining 275.0ml @17:54:08.54 Intake.select :open @17:54:10.72 Intake @16.3psia, vacuum from 9.8 to 13.8psi @17:54:10.74 SS.reconfigure SSinhale.with maxSpeed: 21 @17:54:10.79 SS.seek 25ml @17:55:35.54 Stroke completed -- waiting for pressure to equalize ... @17:55:54.05 Intake.select :close @17:55:54.21 SSV.dial :exhaust @17:55:55.07 Exhaust.select :open @17:55:55.19 SS.reconfigure SSexhale @17:55:55.84 SS.seek 0ml @17:56:05.55 Exhaust.select :close @17:56:05.67 SSV.dial CBV @17:56:05.73 CBV.dial SSV @17:56:06.54 Allowing 130s for 25.0ml of remaining 250.0ml @17:56:06.56 Intake.select :open @17:56:08.74 Intake @16.3psia, vacuum from 9.8 to 13.8psi @17:56:08.76 SS.reconfigure SSinhale.with maxSpeed: 21 @17:56:08.81 SS.seek 25ml @17:57:33.62 Stroke completed -- waiting for pressure to equalize ... @17:57:52.06 Intake.select :close @17:57:52.22 SSV.dial :exhaust @17:57:53.09 Exhaust.select :open @17:57:53.21 SS.reconfigure SSexhale @17:57:53.26 SS.seek 0ml @17:58:03.05 Exhaust.select :close @17:58:03.17 SSV.dial CBV @17:58:03.20 CBV.dial SSV @17:58:04.07 Allowing 130s for 25.0ml of remaining 225.0ml @17:58:04.09 Intake.select :open @17:58:06.27 Intake @16.2psia, vacuum from 9.7 to 13.7psi @17:58:06.29 SS.reconfigure SSinhale.with maxSpeed: 21 @17:58:06.34 SS.seek 25ml @17:59:31.38 Stroke completed -- waiting for pressure to equalize ... @17:59:49.85 Intake.select :close @17:59:50.02 SSV.dial :exhaust @17:59:50.88 Exhaust.select :open @17:59:51.00 SS.reconfigure SSexhale @17:59:51.05 SS.seek 0ml @18:00:00.66 Exhaust.select :close @18:00:00.78 SSV.dial CBV @18:00:00.81 CBV.dial SSV @18:00:01.66 Allowing 130s for 25.0ml of remaining 200.0ml @18:00:01.68 Intake.select :open @18:00:03.86 Intake @16.2psia, vacuum from 9.7 to 13.7psi @18:00:03.88 SS.reconfigure SSinhale.with maxSpeed: 21 @18:00:03.94 SS.seek 25ml @18:01:29.04 Stroke completed -- waiting for pressure to equalize ... @18:01:47.48 Intake.select :close @18:01:47.64 SSV.dial :exhaust @18:01:48.51 Exhaust.select :open @18:01:48.64 SS.reconfigure SSexhale @18:01:48.69 SS.seek 0ml @18:01:58.44 Exhaust.select :close @18:01:58.56 SSV.dial CBV @18:01:58.59 CBV.dial SSV @18:01:59.44 Allowing 130s for 25.0ml of remaining 175.0ml @18:01:59.46 Intake.select :open @18:02:01.64 Intake @16.3psia, vacuum from 9.8 to 13.8psi @18:02:01.66 SS.reconfigure SSinhale.with maxSpeed: 21 @18:02:01.71 SS.seek 25ml @18:03:26.30 Stroke completed -- waiting for pressure to equalize ... @18:03:44.22 Intake.select :close @18:03:44.37 SSV.dial :exhaust @18:03:45.22 Exhaust.select :open @18:03:45.34 SS.reconfigure SSexhale @18:03:45.39 SS.seek 0ml @18:03:55.16 Exhaust.select :close @18:03:55.87 SSV.dial CBV @18:03:55.90 CBV.dial SSV @18:03:56.77 Allowing 130s for 25.0ml of remaining 150.0ml @18:03:56.79 Intake.select :open @18:03:58.97 Intake @16.4psia, vacuum from 9.9 to 13.9psi @18:03:58.99 SS.reconfigure SSinhale.with maxSpeed: 21 @18:03:59.04 SS.seek 25ml @18:05:24.05 Stroke completed -- waiting for pressure to equalize ... @18:05:42.52 Intake.select :close @18:05:42.68 SSV.dial :exhaust @18:05:43.54 Exhaust.select :open @18:05:43.67 SS.reconfigure SSexhale @18:05:43.72 SS.seek 0ml @18:05:53.49 Exhaust.select :close @18:05:53.61 SSV.dial CBV @18:05:53.64 CBV.dial SSV @18:05:54.50 Allowing 130s for 25.0ml of remaining 125.0ml @18:05:54.52 Intake.select :open @18:05:56.69 Intake @16.4psia, vacuum from 9.9 to 13.9psi @18:05:56.72 SS.reconfigure SSinhale.with maxSpeed: 21 @18:05:56.76 SS.seek 25ml @18:07:21.36 Stroke completed -- waiting for pressure to equalize ... @18:07:39.29 Intake.select :close @18:07:39.45 SSV.dial :exhaust @18:07:40.30 Exhaust.select :open @18:07:40.42 SS.reconfigure SSexhale @18:07:40.47 SS.seek 0ml @18:07:50.13 Exhaust.select :close @18:07:50.25 SSV.dial CBV @18:07:50.28 CBV.dial SSV @18:07:51.13 Allowing 130s for 25.0ml of remaining 100.0ml @18:07:51.15 Intake.select :open @18:07:53.33 Intake @16.4psia, vacuum from 9.9 to 13.9psi @18:07:53.35 SS.reconfigure SSinhale.with maxSpeed: 21 @18:07:53.40 SS.seek 25ml @18:09:18.33 Stroke completed -- waiting for pressure to equalize ... @18:09:37.39 Intake.select :close @18:09:37.55 SSV.dial :exhaust @18:09:38.42 Exhaust.select :open @18:09:38.54 SS.reconfigure SSexhale @18:09:38.59 SS.seek 0ml @18:09:48.19 Exhaust.select :close @18:09:48.31 SSV.dial CBV @18:09:48.34 CBV.dial SSV @18:09:49.22 Allowing 130s for 25.0ml of remaining 75.0ml @18:09:49.24 Intake.select :open @18:09:51.42 Intake @16.4psia, vacuum from 9.9 to 13.9psi @18:09:51.44 SS.reconfigure SSinhale.with maxSpeed: 21 @18:09:51.48 SS.seek 25ml @18:11:16.28 Stroke completed -- waiting for pressure to equalize ... @18:11:34.78 Intake.select :close @18:11:34.94 SSV.dial :exhaust @18:11:35.80 Exhaust.select :open @18:11:35.92 SS.reconfigure SSexhale @18:11:35.97 SS.seek 0ml @18:11:45.69 Exhaust.select :close @18:11:45.81 SSV.dial CBV @18:11:45.84 CBV.dial SSV @18:11:47.30 Allowing 130s for 25.0ml of remaining 50.0ml @18:11:47.32 Intake.select :open @18:11:49.50 Intake @16.4psia, vacuum from 9.9 to 13.9psi @18:11:49.52 SS.reconfigure SSinhale.with maxSpeed: 21 @18:11:49.57 SS.seek 25ml @18:13:14.30 Stroke completed -- waiting for pressure to equalize ... @18:13:32.74 Intake.select :close @18:13:32.91 SSV.dial :exhaust @18:13:33.77 Exhaust.select :open @18:13:33.90 SS.reconfigure SSexhale @18:13:33.95 SS.seek 0ml @18:13:43.72 Exhaust.select :close @18:13:43.84 SSV.dial CBV @18:13:43.87 CBV.dial SSV @18:13:44.75 Allowing 130s for 25.0ml of remaining 25.0ml @18:13:44.77 Intake.select :open @18:13:46.94 Intake @16.4psia, vacuum from 9.9 to 13.9psi @18:13:46.96 SS.reconfigure SSinhale.with maxSpeed: 21 @18:13:47.01 SS.seek 24.96ml @18:15:11.56 Stroke completed -- waiting for pressure to equalize ... @18:15:29.96 Intake.select :close @18:15:30.12 SSV.dial :exhaust @18:15:30.99 Exhaust.select :open @18:15:31.11 SS.reconfigure SSexhale @18:15:31.16 SS.seek 0ml @18:15:40.72 Exhaust.select :close @18:15:40.83 Sampled 1200.0ml @18:15:40.85 Drying puck before fixing for 4:00 @18:15:40.86 CBV.dial :waste2b @18:15:41.75 delay 1:00 @18:16:41.78 CSV.dial :air @18:16:42.29 CS.reconfigure CSfast @18:16:42.34 CS.seek 10ml @18:17:09.05 Pumping wcr puck from tube 2 up to 55psi... @18:17:09.07 CBV.dial SSV @18:17:10.51 CBV.dial between CSV and waste2b @18:17:11.62 CSV.dial between air and CTV @18:17:12.00 CS.seek 7.018ml @18:17:20.52 CTV.dial CSV,avoiding: :air @18:17:20.56 CSV.dial CTV @18:17:21.45 CS.reconfigure CSpressurize @18:17:21.50 CS.seek 0ml @18:17:37.23 CBV.dial :waste2b @18:17:37.61 CS.reconfigure CSconfig @18:17:37.66 CS.seek :empty @18:17:41.64 delay 1:00 @18:18:41.67 CTV.dial :air,avoiding: :intake @18:18:43.18 Fix #1 of 2 (with rnal) for 2:00 @18:18:43.19 CSV.dial CSR @18:18:44.34 Pulling 2ml of CSR.rnal @18:18:44.35 CSR.select :rnal @18:18:44.49 CS.seek 2ml @18:18:50.58 delay 5 seconds @18:18:55.61 CSR.select :air @18:18:55.74 CS.reconfigure CSfast @18:18:55.79 CS.seek 10ml @18:19:17.14 Pumping wcr puck from tube 2 up to 55psi... @18:19:17.16 CBV.dial SSV @18:19:18.57 CBV.dial between CSV and waste2b @18:19:19.66 CSV.dial between air and CTV @18:19:20.37 CS.seek 6.982ml @18:19:29.22 CTV.dial CSV,avoiding: :air @18:19:29.25 CSV.dial CTV @18:19:30.83 CS.reconfigure CSpressurize @18:19:30.88 CS.seek 0ml @18:19:47.92 delay 2:00 @18:21:47.95 CBV.dial :waste2b @18:21:48.33 CS.reconfigure CSconfig @18:21:48.38 CS.seek :empty @18:21:50.80 delay 1:00 @18:22:50.83 CTV.dial :air,avoiding: :intake @18:22:52.34 Fix #2 of 2 (with rnal) for 2:00 @18:22:52.35 CSV.dial CSR @18:22:52.92 Pulling 2ml of CSR.rnal @18:22:52.93 CSR.select :rnal @18:22:53.06 CS.seek 2ml @18:22:59.55 delay 5 seconds @18:23:04.58 CSR.select :air @18:23:04.71 CS.reconfigure CSfast @18:23:04.76 CS.seek 10ml @18:23:26.21 Pumping wcr puck from tube 2 up to 55psi... @18:23:26.23 CBV.dial SSV @18:23:27.64 CBV.dial between CSV and waste2b @18:23:28.73 CSV.dial between air and CTV @18:23:29.44 CS.seek 6.974ml @18:23:38.41 CTV.dial CSV,avoiding: :air @18:23:38.44 CSV.dial CTV @18:23:40.00 CS.reconfigure CSpressurize @18:23:40.05 CS.seek 0ml @18:23:56.35 delay 2:00 @18:25:56.38 CBV.dial :waste2b @18:25:56.74 CS.reconfigure CSconfig @18:25:56.80 CS.seek :empty @18:26:00.21 delay 1:00 @18:27:00.24 CTV.dial :air,avoiding: :intake @18:27:01.83 Storing wcr puck in Collection Clamp to tube 1 @18:27:02.03 SC.reconfigure SCtight @18:27:02.09 SC.seek 1 @18:27:04.27 SC.reconfigure SCloose @18:27:04.52 Elbow.reconfigure TightElbow @18:27:04.57 Elbow.seek 1 @18:27:06.48 Forearm.reconfigure TightForearm @18:27:06.53 Forearm.seek 1 + 700 counts @18:27:07.57 SE.reconfigure SEpick @18:27:07.65 SE.seek beamBreak @18:27:10.79 SE.configure SEconfig @18:27:10.97 Elbow.reconfigure LimpElbow @18:27:11.03 Forearm.reconfigure LooseForearm @18:27:11.08 Forearm.seek 1 @18:27:11.41 Elbow.hold @18:27:11.49 Elbow.reconfigure TightElbow @18:27:11.61 SE.seek 1.07 Pucks below Top Plate @18:27:12.15 Forearm.force -20 @18:27:12.20 Forearm.seek 1 + 339 counts @18:27:12.53 Forearm.reconfigure TightForearm @18:27:12.59 Forearm.seek 1 + 700 counts @18:27:12.83 SE.seek 2.07 Pucks below Top Plate @18:27:13.67 Forearm.reconfigure LooseForearm @18:27:13.72 Forearm.seek :retract @18:27:14.66 Elbow.seek CC @18:27:16.36 Elbow.reconfigure LooseElbow @18:27:16.48 Forearm.seek CC @18:27:17.47 Hand.close @18:27:18.17 CC.seek :open @18:27:22.70 Forearm.seek :retract @18:27:23.76 Elbow.reconfigure TightElbow @18:27:23.81 Elbow.seek 1 @18:27:26.36 Elbow.reconfigure LimpElbow @18:27:26.42 Forearm.seek 1 @18:27:27.46 Elbow.hold @18:27:27.54 Elbow.reconfigure TightElbow @18:27:27.59 Hand.open @18:27:28.44 Forearm.force -20 @18:27:28.49 Forearm.seek 1 + 349 counts @18:27:28.83 Forearm.reconfigure TightForearm @18:27:28.88 Forearm.seek 1 + 700 counts @18:27:29.20 SE.seek :down @18:27:30.37 Forearm.reconfigure LooseForearm @18:27:30.43 Forearm.seek :retract @18:27:31.98 SE.coast @18:27:32.02 SC.coast @18:27:32.06 Starting finalPurge @18:27:32.08 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:27:32.16 Hand.open @18:27:32.56 Elbow.seek FlushPuck::Garage @18:27:34.50 Elbow.reconfigure LooseElbow @18:27:34.61 Forearm.seek :garage @18:27:35.63 Hand.close @18:27:36.55 Forearm.seek :retract @18:27:37.60 Elbow.reconfigure TightElbow @18:27:37.64 Elbow.seek CC @18:27:40.56 Elbow.reconfigure LooseElbow @18:27:40.67 Forearm.reconfigure TightForearm @18:27:40.72 Forearm.seek CC @18:27:42.00 CC.seek :closed @18:27:46.27 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.28 @18:27:46.35 Hand.open @18:27:47.12 Forearm.reconfigure LooseForearm @18:27:47.17 Forearm.seek :retract @18:27:48.15 Flushing the Collection Valve::Manifold @18:27:48.30 CSV.dial CSR @18:27:48.88 Pulling 5ml of CSR.flush @18:27:48.89 CSR.select :flush @18:27:49.01 CS.seek 5ml @18:28:02.80 delay 5 seconds @18:28:07.83 CSR.select :air @18:28:08.00 CS.reconfigure CSfast @18:28:08.05 CS.seek 7.5ml @18:28:15.37 CSV.dial :waste @18:28:16.96 CS.reconfigure CSconfig @18:28:17.59 CS.seek 0ml @18:28:37.65 Flushing lower manifold's waste1 port @18:28:37.80 CSV.dial :flush @18:28:38.33 CS.reconfigure CSfast @18:28:38.38 CS.seek 5ml @18:28:52.43 delay 5 seconds @18:28:57.45 CSV.dial :air @18:28:58.04 CS.seek 7ml @18:29:04.12 CSV.dial CTV @18:29:04.16
CTV.dial CSV @18:29:05.76 CBV.dial :waste1 @18:29:06.64 CS.seek 0ml @18:29:25.52 Flushing lower manifold's waste2b port @18:29:25.67 CSV.dial :flush @18:29:26.50 CS.seek 5ml @18:29:40.12 delay 5 seconds @18:29:45.14 CSV.dial :air @18:29:45.74 CS.seek 7ml @18:29:51.74 CSV.dial CTV @18:29:51.78
CTV.dial CSV @18:29:52.27 CBV.dial :waste2b @18:29:53.14 CS.seek 0ml @18:30:11.87 Flushing lower manifold's waste1 port @18:30:11.89 CSV.dial CSR @18:30:12.45 Pulling 4ml of CSR.mfbkill @18:30:12.46 CSR.select :mfbkill @18:30:12.58 CS.reconfigure CSconfig @18:30:12.63 CS.seek 4ml @18:30:24.02 delay 5 seconds @18:30:29.05 CSR.select :air @18:30:29.18 CS.reconfigure CSfast @18:30:29.23 CS.seek 10ml @18:30:45.50 CSV.dial CTV @18:30:45.53
CTV.dial CSV @18:30:46.06 CBV.dial :waste1 @18:30:46.92 CS.seek 0ml @18:31:13.24 Flushing lower manifold's waste2b port @18:31:13.26 CSV.dial CSR @18:31:13.83 Pulling 4ml of CSR.mfbkill @18:31:13.84 CSR.select :mfbkill @18:31:13.97 CS.reconfigure CSconfig @18:31:14.06 CS.seek 4ml @18:31:25.37 delay 5 seconds @18:31:30.39 CSR.select :air @18:31:30.52 CS.reconfigure CSfast @18:31:30.57 CS.seek 10ml @18:31:46.82 CSV.dial CTV @18:31:46.85
CTV.dial CSV @18:31:47.37 CBV.dial :waste2b @18:31:48.24 CS.seek 0ml @18:32:14.53 Killing upper manifold @18:32:14.54 CSV.dial CSR @18:32:15.13 Pulling 4ml of CSR.mfbkill @18:32:15.14 CSR.select :mfbkill @18:32:15.26 CS.reconfigure CSconfig @18:32:15.31 CS.seek 4ml @18:32:26.71 delay 5 seconds @18:32:31.73 CSR.select :air @18:32:31.86 CS.reconfigure CSfast @18:32:31.91 CS.seek 10ml @18:32:48.24 CSV.dial CBV @18:32:48.28
CBV.dial CSV @18:32:49.48 CTV.dial :air @18:32:51.00 CS.seek 0ml @18:33:17.59 delay 7 seconds @18:33:24.62 CS.seek 3ml @18:33:33.28 CSV.dial :waste @18:33:33.79 CS.seek 0ml @18:33:42.34 Flushing upper manifold @18:33:42.48 CSV.dial :flush @18:33:42.99 CS.seek 5ml @18:33:56.59 delay 5 seconds @18:34:01.61 CSV.dial :air @18:34:02.31 CS.seek 7.001ml @18:34:08.44 CSV.dial CBV @18:34:08.47
CBV.dial CSV @18:34:09.62 CTV.dial :air @18:34:09.65 CS.seek 0ml @18:34:28.25 delay 7 seconds @18:34:35.28 CS.seek 3ml @18:34:43.90 CSV.dial :waste @18:34:44.54 CS.seek 0ml @18:34:53.22 CSV.dial :flush @18:34:53.74 CS.seek 3ml @18:35:02.37 delay 5 seconds @18:35:07.39 CSV.dial :air @18:35:08.09 CS.seek 5ml @18:35:14.15 CSV.dial :air @18:35:14.17 CS.seek 0ml @18:35:27.75 delay 7 seconds @18:35:34.78 CS.seek 3ml @18:35:43.28 CSV.dial :waste @18:35:44.12 CS.seek 0ml @18:35:52.65 Flushing lower manifold's waste1 port @18:35:52.79 CSV.dial :flush @18:35:53.32 CS.seek 5ml @18:36:07.03 delay 5 seconds @18:36:12.05 CSV.dial :air @18:36:12.64 CS.seek 7ml @18:36:18.76 CSV.dial CTV @18:36:18.79
CTV.dial CSV @18:36:20.36 CBV.dial :waste1 @18:36:20.88 CS.seek 0ml @18:36:39.53 Flushing lower manifold's waste2b port @18:36:39.68 CSV.dial :flush @18:36:40.52 CS.seek 5ml @18:36:54.18 delay 5 seconds @18:36:59.20 CSV.dial :air @18:36:59.80 CS.seek 7ml @18:37:05.81 CSV.dial CTV @18:37:05.85
CTV.dial CSV @18:37:06.34 CBV.dial :waste2b @18:37:07.78 CS.seek 0ml @18:37:26.47 Garaging FlushPuck in Collection Clamp @18:37:26.56 Elbow.reconfigure TightElbow @18:37:26.61 Elbow.seek CC @18:37:26.91 Elbow.reconfigure LooseElbow @18:37:27.02 Forearm.seek CC @18:37:27.99 Hand.close @18:37:28.81 CC.seek :open @18:37:33.23 Forearm.seek :retract @18:37:34.25 Elbow.reconfigure TightElbow @18:37:34.30 Elbow.seek :garage @18:37:37.32 Elbow.reconfigure LooseElbow @18:37:37.44 Forearm.reconfigure TightForearm @18:37:37.49 Forearm.seek :garage @18:37:38.67 Hand.open @18:37:39.50 Forearm.reconfigure LooseForearm @18:37:39.55 Forearm.seek :retract @18:37:40.63 Starting finalPurge @18:37:40.65 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:37:40.72 Hand.open @18:37:40.99 Elbow.reconfigure TightElbow @18:37:41.04 Elbow.seek FlushPuck::Garage @18:37:41.17 Elbow.reconfigure LooseElbow @18:37:41.29 Forearm.seek :garage @18:37:42.34 Hand.close @18:37:43.19 Forearm.seek :retract @18:37:44.25 Elbow.reconfigure TightElbow @18:37:44.30 Elbow.seek CC @18:37:47.20 Elbow.reconfigure LooseElbow @18:37:47.30 Forearm.reconfigure TightForearm @18:37:47.35 Forearm.seek CC @18:37:48.68 CC.seek :closed @18:37:52.92 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.32 @18:37:53.01 Hand.open @18:37:53.87 Forearm.reconfigure LooseForearm @18:37:53.92 Forearm.seek :retract @18:37:54.99 Flushing the Collection Valve::Manifold @18:37:55.13 CSV.dial CSR @18:37:55.69 Pulling 5ml of CSR.flush @18:37:55.70 CSR.select :flush @18:37:55.82 CS.reconfigure CSconfig @18:37:55.87 CS.seek 5ml @18:38:09.69 delay 5 seconds @18:38:14.72 CSR.select :air @18:38:14.90 CS.reconfigure CSfast @18:38:14.95 CS.seek 7.5ml @18:38:22.25 CSV.dial :waste @18:38:23.73 CS.reconfigure CSconfig @18:38:23.78 CS.seek 0ml @18:38:44.00 Flushing lower manifold's waste1 port @18:38:44.14 CSV.dial :flush @18:38:45.22 CS.reconfigure CSfast @18:38:45.29 CS.seek 5ml @18:38:58.90 delay 5 seconds @18:39:03.92 CSV.dial :air @18:39:04.50 CS.seek 7ml @18:39:10.57 CSV.dial CTV @18:39:10.60
CTV.dial CSV @18:39:11.09 CBV.dial :waste1 @18:39:11.96 CS.seek 0ml @18:39:30.65 Flushing lower manifold's waste2b port @18:39:30.79 CSV.dial :flush @18:39:31.65 CS.seek 5ml @18:39:45.31 delay 5 seconds @18:39:50.33 CSV.dial :air @18:39:50.92 CS.seek 7ml @18:39:56.90 CSV.dial CTV @18:39:56.94
CTV.dial CSV @18:39:57.44 CBV.dial :waste2b @18:39:58.30 CS.seek 0ml @18:40:16.78 Disinfecting external Intake with kill @18:40:16.93 CSV.dial CSR @18:40:17.51 Pulling 8ml of CSR.kill @18:40:17.52 CSR.select :kill @18:40:17.64 CS.seek 8ml @18:40:39.03 delay 5 seconds @18:40:44.06 CSR.select :air @18:40:44.19 CS.seek 10ml @18:40:50.25 CSV.dial CBV @18:40:50.29
CBV.dial CSV @18:40:51.48 CTV.dial :intake @18:40:52.33 Intake.select :open @18:40:52.45 CS.seek 2ml @18:41:14.31 Intake.select :close @18:41:14.42 CTV.dial :air @18:41:15.26 CS.reconfigure CSconfig @18:41:15.32 CS.seek 0ml @18:41:21.34 CSV.dial CSR @18:41:22.55 Pulling 5ml of CSR.flush CSR.select :flush @18:41:22.68 CS.seek 5ml @18:41:36.47 delay 5 seconds @18:41:41.49 CSR.select :air @18:41:41.62 CS.seek 10ml @18:41:55.53 CSV.dial CBV @18:41:56.68 CS.seek 0ml @18:42:23.25 Garaging FlushPuck in Collection Clamp @18:42:23.33 Elbow.reconfigure TightElbow @18:42:23.38 Elbow.seek CC @18:42:23.65 Elbow.reconfigure LooseElbow @18:42:23.76 Forearm.seek CC @18:42:24.76 Hand.close @18:42:25.56 CC.seek :open @18:42:29.95 Forearm.seek :retract @18:42:30.91 Elbow.reconfigure TightElbow @18:42:30.96 Elbow.seek :garage @18:42:34.01 Elbow.reconfigure LooseElbow @18:42:34.12 Forearm.reconfigure TightForearm @18:42:34.17 Forearm.seek :garage @18:42:35.34 Hand.open @18:42:36.12 Forearm.reconfigure LooseForearm @18:42:36.17 Forearm.seek :retract @18:42:37.80 Completed [mbaPhase] begun 14:43:39 @18:42:37.82 Extending 18tun1 until completion of MFBPCR @19:08:26.28 PCR.off @19:08:27.88 PCR for Tt1 completed -- closed /var/log/esp/Tt118sep1217h1500ml.pcr @19:08:27.96 Cleaning PCR @19:08:27.97 AFV.select :carrierSolution @19:08:28.08 delay 2 seconds @19:08:30.12 AS.reconfigure ASconfig @19:08:30.16 AS.seek 0.5ml @19:08:40.48 delay 1 second @19:08:41.50 AFV.select :holdingCoil @19:08:41.60 ASV.dial :waste1 @19:08:41.98 ATV.dial :air3 @19:08:43.25 AS.reconfigure ASslow1 @19:08:43.30 AS.seek 0.51ml @19:08:45.70 delay 2 seconds @19:08:47.72 ATV.dial :bleach @19:08:48.35 AS.seek 0.81ml @19:09:49.06 delay 3 seconds @19:09:52.09 ATV.dial :air3 @19:09:52.63 delay 3 seconds @19:09:55.72 AS.seek 0.835ml @19:10:01.14 delay 3 seconds @19:10:04.17 ATV.dial :bleach @19:10:04.80 AS.seek 0.82ml @19:10:08.10 delay 3 seconds @19:10:11.13 ARV.dial :waste @19:10:11.74 delay 3 seconds @19:10:14.77 ATV.dial :ARV @19:10:15.64 AS.reconfigure ASconfig @19:10:15.69 AS.seek 0.47ml @19:10:36.12 AFV.select :carrierSolution @19:10:36.23 delay 2 seconds @19:10:38.27 AS.seek 1ml @19:11:09.04 delay 1 second @19:11:10.06 AFV.select :holdingCoil @19:11:10.16 delay 1 second @19:11:11.20 AS.reconfigure ASfast @19:11:11.24 AS.seek 0ml @19:11:38.62 AFV.select :carrierSolution @19:11:38.73 delay 2 seconds @19:11:40.77 AS.reconfigure ASconfig @19:11:40.81 AS.seek 1ml @19:12:38.63 delay 1 second @19:12:39.65 AFV.select :holdingCoil @19:12:39.75 delay 1 second @19:12:40.79 AS.reconfigure ASfast @19:12:40.83 AS.seek 0ml @19:13:08.21 AFV.select :carrierSolution @19:13:08.32 delay 2 seconds @19:13:10.36 AS.reconfigure ASconfig @19:13:10.40 AS.seek 1ml @19:14:08.20 delay 1 second @19:14:09.22 AFV.select :holdingCoil @19:14:09.32 delay 1 second @19:14:10.36 AS.reconfigure ASfast @19:14:10.40 AS.seek 0ml @19:14:37.76 ARV.dial between carrier and air1 @19:14:38.28 ATV.dial :air3 @19:14:39.55 AS.reconfigure ASslow1 @19:14:39.60 AS.seek 0.01ml @19:14:41.85 delay 2 seconds @19:14:43.87 ATV.dial :bleach @19:14:45.04 AS.seek 0.81ml @19:17:26.23 delay 3 seconds @19:17:29.26 ATV.dial :air3 @19:17:29.82 delay 3 seconds @19:17:32.92 AS.seek 0.835ml @19:17:38.25 delay 3 seconds @19:17:41.28 ATV.dial :bleach @19:17:41.92 AS.seek 0.82ml @19:17:45.23 delay 3 seconds @19:17:48.26 ATV.dial PCR @19:17:48.68 AS.reconfigure ASconfig @19:17:48.73 AS.seek :empty @19:18:36.20 PCR.program :bleachProfile @19:18:36.26 PCR program should complete in < 2:45 @19:18:36.27 PCR.run 10:00 @19:21:41.12 PCR.off @19:21:41.16 AFV.select :carrierSolution @19:21:41.27 delay 2 seconds @19:21:43.31 AS.seek 1ml @19:22:41.13 delay 1 second @19:22:42.15 AFV.select :holdingCoil @19:22:42.25 delay 1 second @19:22:43.29 AS.reconfigure ASfast @19:22:43.33 AS.seek 0ml @19:23:10.68 delay 3 seconds @19:23:13.71 AFV.select :carrierSolution @19:23:13.81 delay 2 seconds @19:23:15.85 AS.reconfigure ASconfig @19:23:15.90 AS.seek 1ml @19:24:13.73 delay 1 second @19:24:14.75 AFV.select :holdingCoil @19:24:14.85 delay 1 second @19:24:15.89 AS.reconfigure ASfast @19:24:15.93 AS.seek 0ml @19:24:43.31 delay 3 seconds @19:24:46.34 AFV.select :carrierSolution @19:24:46.44 delay 2 seconds @19:24:48.48 AS.reconfigure ASconfig @19:24:48.53 AS.seek 1ml @19:25:46.27 delay 1 second @19:25:47.29 AFV.select :holdingCoil @19:25:47.39 delay 1 second @19:25:48.43 AS.reconfigure ASfast @19:25:48.47 AS.seek 0ml @19:26:15.82 delay 3 seconds @19:26:18.85 AFV.select :carrierSolution @19:26:18.95 delay 2 seconds @19:26:20.99 AS.reconfigure ASconfig @19:26:21.04 AS.seek 1ml @19:27:18.85 delay 1 second @19:27:19.87 AFV.select :holdingCoil @19:27:19.97 delay 1 second @19:27:21.00 AS.reconfigure ASfast @19:27:21.05 AS.seek 0ml @19:27:48.40 delay 3 seconds @19:27:51.43 AFV.select :carrierSolution @19:27:51.53 delay 2 seconds @19:27:53.57 AS.reconfigure ASconfig @19:27:53.62 AS.seek 1ml @19:28:51.41 delay 1 second @19:28:52.43 AFV.select :holdingCoil @19:28:52.53 delay 1 second @19:28:53.57 AS.reconfigure ASnorm @19:28:53.61 AS.seek 0ml @19:29:44.34 Setting up PCR for Rp -- writing /var/log/esp/Rp18sep1219h1500ml.pcr @19:29:44.36 Building PCR Slug from ASV with MM2 mix, PP3 primer @19:29:44.38 Priming with carrier @19:29:44.39 ATV.dial ARV @19:29:44.99 ARV.dial :carrier @19:29:45.34 AS.seek 0.5ml @19:30:10.96 AFV.select :carrierSolution @19:30:11.07 delay 2 seconds @19:30:13.10 AS.reconfigure ASconfig @19:30:13.15 AS.seek 1ml @19:30:42.23 delay 1 second @19:30:43.25 AFV.select :holdingCoil @19:30:43.35 delay 1 second @19:30:44.38 ATV.dial :waste @19:30:46.03 AS.reconfigure ASnorm @19:30:46.07 AS.seek :empty @19:31:36.77 AFV.select :carrierSolution @19:31:36.88 delay 2 seconds @19:31:38.92 AS.reconfigure ASconfig @19:31:38.96 AS.seek 0.5ml @19:32:08.09 delay 1 second @19:32:09.11 AFV.select :holdingCoil @19:32:09.21 Building Slug @19:32:09.23 ATV.dial :ARV @19:32:10.82 delay 2 seconds @19:32:12.85 ARV.dial :air1 @19:32:13.24 delay 2 seconds @19:32:15.33 AS.reconfigure ASslow2 @19:32:15.38 AS.seek 0.506ml @19:32:21.63 delay 2 seconds @19:32:23.66 ARV.dial :MM2 @19:32:25.16 delay 2 seconds @19:32:27.19 Adding MM2 @19:32:27.27 AS.seek 0.524ml @19:32:45.50 delay 25 seconds @19:33:10.53 ARV.dial :PP3 @19:33:10.93 Adding PP3 @19:33:10.95 delay 2 seconds @19:33:13.04 AS.seek 0.53ml @19:33:19.32 delay 10 seconds @19:33:29.35 ARV.dial :air2 @19:33:29.74 adding ARV air @19:33:29.76 delay 2 seconds @19:33:31.85 AS.seek 0.536ml @19:33:38.01 delay 5 seconds @19:33:43.04 ARV.dial :carrier @19:33:44.34 delay 7 seconds @19:33:51.44 AS.seek 0.594ml @19:34:49.99 delay 5 seconds @19:34:55.02 ATV.dial :ASV @19:34:55.41 delay 2 seconds @19:34:57.50 AS.seek 0.6ml @19:35:04.12 delay 4 seconds @19:35:08.15 ATV.dial ARV @19:35:08.70 delay 2 seconds @19:35:10.73 ARV.dial :carrier @19:35:10.74 delay 2 seconds @19:35:12.84 AS.seek 0.65ml @19:36:03.41 delay 4 seconds @19:36:07.44 ATV.dial PCR @19:36:08.04 ASV.dial :waste1 @19:36:08.06 ARV.dial :air1 @19:36:08.48 Positioning Slug by 0.324ml @19:36:08.57 AS.seek 0.326ml @19:41:34.66 delay 5 seconds @19:41:39.69 ATV.dial ARV @19:41:40.43 ASV.dial :PCRlock @19:41:40.82 delay 5 seconds @19:41:45.85 Running Rp Profile60C300 thermocycling @19:41:45.87 PCR.program Profile60C300 @19:41:46.00 Profile60C300 should complete in < 1:45:21 @19:41:46.02 PCR.run 2:16:41.25 @21:07:00.30 PCR.off @21:07:01.91 PCR for Rp completed -- closed /var/log/esp/Rp18sep1219h1500ml.pcr @21:07:01.98 Cleaning PCR @21:07:01.99 AFV.select :carrierSolution @21:07:02.10 delay 2 seconds @21:07:04.14 AS.reconfigure ASconfig @21:07:04.18 AS.seek 0.5ml @21:07:14.49 delay 1 second @21:07:15.51 AFV.select :holdingCoil @21:07:15.61 ASV.dial :waste1 @21:07:15.99 ATV.dial :air3 @21:07:17.26 AS.reconfigure ASslow1 @21:07:17.31 AS.seek 0.51ml @21:07:19.69 delay 2 seconds @21:07:21.71 ATV.dial :bleach @21:07:22.34 AS.seek 0.81ml @21:08:22.96 delay 3 seconds @21:08:25.99 ATV.dial :air3 @21:08:26.54 delay 3 seconds @21:08:29.63 AS.seek 0.835ml @21:08:34.97 delay 3 seconds @21:08:38.00 ATV.dial :bleach @21:08:38.62 AS.seek 0.82ml @21:08:41.96 delay 3 seconds @21:08:44.98 ARV.dial :waste @21:08:45.60 delay 3 seconds @21:08:48.63 ATV.dial :ARV @21:08:49.51 AS.reconfigure ASconfig @21:08:49.56 AS.seek 0.47ml @21:09:09.96 AFV.select :carrierSolution @21:09:10.07 delay 2 seconds @21:09:12.11 AS.seek 1ml @21:09:42.88 delay 1 second @21:09:43.90 AFV.select :holdingCoil @21:09:44.00 delay 1 second @21:09:45.04 AS.reconfigure ASfast @21:09:45.08 AS.seek 0ml @21:10:12.46 AFV.select :carrierSolution @21:10:12.57 delay 2 seconds @21:10:14.61 AS.reconfigure ASconfig @21:10:14.65 AS.seek 1ml @21:11:12.44 delay 1 second @21:11:13.46 AFV.select :holdingCoil @21:11:13.56 delay 1 second @21:11:14.60 AS.reconfigure ASfast @21:11:14.64 AS.seek 0ml @21:11:42.00 AFV.select :carrierSolution @21:11:42.11 delay 2 seconds @21:11:44.15 AS.reconfigure ASconfig @21:11:44.19 AS.seek 1ml @21:12:41.99 delay 1 second @21:12:43.01 AFV.select :holdingCoil @21:12:43.11 delay 1 second @21:12:44.15 AS.reconfigure ASfast @21:12:44.19 AS.seek 0ml @21:13:11.55 ARV.dial between carrier and air1 @21:13:12.61 ATV.dial :air3 @21:13:13.90 AS.reconfigure ASslow1 @21:13:13.95 AS.seek 0.01ml @21:13:16.25 delay 2 seconds @21:13:18.27 ATV.dial :bleach @21:13:18.90 AS.seek 0.81ml @21:16:00.03 delay 3 seconds @21:16:03.06 ATV.dial :air3 @21:16:03.60 delay 3 seconds @21:16:06.69 AS.seek 0.835ml @21:16:12.00 delay 3 seconds @21:16:15.03 ATV.dial :bleach @21:16:15.65 AS.seek 0.82ml @21:16:19.05 delay 3 seconds @21:16:22.08 ATV.dial PCR @21:16:22.46 AS.reconfigure ASconfig @21:16:22.51 AS.seek :empty @21:17:09.99 PCR.program :bleachProfile @21:17:10.05 PCR program should complete in < 2:45 @21:17:10.06 PCR.run 10:00 @21:20:14.92 PCR.off @21:20:14.95 AFV.select :carrierSolution @21:20:15.05 delay 2 seconds @21:20:17.09 AS.seek 1ml @21:21:14.88 delay 1 second @21:21:15.90 AFV.select :holdingCoil @21:21:16.00 delay 1 second @21:21:17.03 AS.reconfigure ASfast @21:21:17.08 AS.seek 0ml @21:21:44.44 delay 3 seconds @21:21:47.47 AFV.select :carrierSolution @21:21:47.57 delay 2 seconds @21:21:49.61 AS.reconfigure ASconfig @21:21:49.66 AS.seek 1ml @21:22:47.50 delay 1 second @21:22:48.52 AFV.select :holdingCoil @21:22:48.62 delay 1 second @21:22:49.66 AS.reconfigure ASfast @21:22:49.70 AS.seek 0ml @21:23:17.05 delay 3 seconds @21:23:20.08 AFV.select :carrierSolution @21:23:20.18 delay 2 seconds @21:23:22.22 AS.reconfigure ASconfig @21:23:22.27 AS.seek 1ml @21:24:20.02 delay 1 second @21:24:21.04 AFV.select :holdingCoil @21:24:21.14 delay 1 second @21:24:22.17 AS.reconfigure ASfast @21:24:22.21 AS.seek 0ml @21:24:49.55 delay 3 seconds @21:24:52.58 AFV.select :carrierSolution @21:24:52.68 delay 2 seconds @21:24:54.72 AS.reconfigure ASconfig @21:24:54.76 AS.seek 1ml @21:25:52.60 delay 1 second @21:25:53.62 AFV.select :holdingCoil @21:25:53.72 delay 1 second @21:25:54.76 AS.reconfigure ASfast @21:25:54.80 AS.seek 0ml @21:26:22.18 delay 3 seconds @21:26:25.20 AFV.select :carrierSolution @21:26:25.30 delay 2 seconds @21:26:27.33 AS.reconfigure ASconfig @21:26:27.39 AS.seek 1ml @21:27:25.22 delay 1 second @21:27:26.25 AFV.select :holdingCoil @21:27:26.35 delay 1 second @21:27:27.39 AS.reconfigure ASnorm @21:27:27.43 AS.seek 0ml @21:28:18.18 Setting up PCR for Ss -- writing /var/log/esp/Ss18sep1221h1500ml.pcr @21:28:18.20 Building PCR Slug from ASV with MM1 mix, PP2 primer @21:28:18.21 Priming with carrier @21:28:18.22 ATV.dial ARV @21:28:18.82 ARV.dial :carrier @21:28:19.17 AS.seek 0.5ml @21:28:44.72 AFV.select :carrierSolution @21:28:44.83 delay 2 seconds @21:28:46.86 AS.reconfigure ASconfig @21:28:46.91 AS.seek 1ml @21:29:15.99 delay 1 second @21:29:17.01 AFV.select :holdingCoil @21:29:17.11 delay 1 second @21:29:18.14 ATV.dial :waste @21:29:19.79 AS.reconfigure ASnorm @21:29:19.84 AS.seek :empty @21:30:10.61 AFV.select :carrierSolution @21:30:10.72 delay 2 seconds @21:30:12.76 AS.reconfigure ASconfig @21:30:12.81 AS.seek 0.5ml @21:30:41.94 delay 1 second @21:30:42.96 AFV.select :holdingCoil @21:30:43.06 Building Slug @21:30:43.07 ATV.dial :ARV @21:30:44.68 delay 2 seconds @21:30:46.71 ARV.dial :air1 @21:30:47.11 delay 2 seconds @21:30:49.20 AS.reconfigure ASslow2 @21:30:49.25 AS.seek 0.506ml @21:30:56.44 delay 2 seconds @21:30:58.46 ARV.dial :MM1 @21:30:59.07 delay 2 seconds @21:31:01.10 Adding MM1 @21:31:01.18 AS.seek 0.524ml @21:31:19.68 delay 25 seconds @21:31:44.71 ARV.dial :PP2 @21:31:45.11 Adding PP2 @21:31:45.13 delay 2 seconds @21:31:47.21 AS.seek 0.53ml @21:31:53.71 delay 10 seconds @21:32:03.74 ARV.dial :air2 @21:32:04.14 adding ARV air @21:32:04.16 delay 2 seconds @21:32:06.24 AS.seek 0.536ml @21:32:12.75 delay 5 seconds @21:32:17.78 ARV.dial :carrier @21:32:19.08 delay 7 seconds @21:32:26.17 AS.seek 0.594ml @21:33:24.69 delay 5 seconds @21:33:29.72 ATV.dial :ASV @21:33:30.11 delay 2 seconds @21:33:32.20 AS.seek 0.6ml @21:33:38.41 delay 4 seconds @21:33:42.44 ATV.dial ARV @21:33:42.97 delay 2 seconds @21:33:45.00 ARV.dial :carrier @21:33:45.01 delay 2 seconds @21:33:47.10 AS.seek 0.65ml @21:34:37.60 delay 4 seconds @21:34:41.63 ATV.dial PCR @21:34:42.24 ASV.dial :waste1 @21:34:42.26 ARV.dial :air1 @21:34:42.68 Positioning Slug by 0.324ml @21:34:42.76 AS.seek 0.326ml @21:40:09.10 delay 5 seconds @21:40:14.13 ATV.dial ARV @21:40:14.89 ASV.dial :PCRlock @21:40:15.27 delay 5 seconds @21:40:20.30 Running Ss Profile60C350 thermocycling @21:40:20.32 PCR.program Profile60C350 @21:40:20.46 Profile60C350 should complete in < 1:48:05 @21:40:20.47 PCR.run 2:20:06.25 @23:05:48.27 PCR.off @23:05:50.35 PCR for Ss completed -- closed /var/log/esp/Ss18sep1221h1500ml.pcr @23:05:50.42 Cleaning PCR @23:05:50.43 AFV.select :carrierSolution @23:05:50.54 delay 2 seconds @23:05:52.57 AS.reconfigure ASconfig @23:05:52.62 AS.seek 0.5ml @23:06:02.92 delay 1 second @23:06:03.94 AFV.select :holdingCoil @23:06:04.04 ASV.dial :waste1 @23:06:04.42 ATV.dial :air3 @23:06:05.69 AS.reconfigure ASslow1 @23:06:05.74 AS.seek 0.51ml @23:06:08.03 delay 2 seconds @23:06:10.05 ATV.dial :bleach @23:06:10.68 AS.seek 0.81ml @23:07:11.27 delay 3 seconds @23:07:14.30 ATV.dial :air3 @23:07:14.86 delay 3 seconds @23:07:17.96 AS.seek 0.835ml @23:07:23.34 delay 3 seconds @23:07:26.37 ATV.dial :bleach @23:07:26.99 AS.seek 0.82ml @23:07:30.28 delay 3 seconds @23:07:33.30 ARV.dial :waste @23:07:33.92 delay 3 seconds @23:07:36.95 ATV.dial :ARV @23:07:37.93 AS.reconfigure ASconfig @23:07:37.98 AS.seek 0.47ml @23:07:58.37 AFV.select :carrierSolution @23:07:58.48 delay 2 seconds @23:08:00.52 AS.seek 1ml @23:08:31.31 delay 1 second @23:08:32.33 AFV.select :holdingCoil @23:08:32.43 delay 1 second @23:08:33.47 AS.reconfigure ASfast @23:08:33.51 AS.seek 0ml @23:09:00.87 AFV.select :carrierSolution @23:09:00.98 delay 2 seconds @23:09:03.02 AS.reconfigure ASconfig @23:09:03.06 AS.seek 1ml @23:10:01.41 delay 1 second @23:10:02.44 AFV.select :holdingCoil @23:10:02.54 delay 1 second @23:10:03.58 AS.reconfigure ASfast @23:10:03.62 AS.seek 0ml @23:10:30.98 AFV.select :carrierSolution @23:10:31.09 delay 2 seconds @23:10:33.13 AS.reconfigure ASconfig @23:10:33.17 AS.seek 1ml @23:11:30.98 delay 1 second @23:11:32.00 AFV.select :holdingCoil @23:11:32.10 delay 1 second @23:11:33.14 AS.reconfigure ASfast @23:11:33.18 AS.seek 0ml @23:12:00.55 ARV.dial between carrier and air1 @23:12:01.05 ATV.dial :air3 @23:12:02.32 AS.reconfigure ASslow1 @23:12:02.37 AS.seek 0.01ml @23:12:04.81 delay 2 seconds @23:12:06.84 ATV.dial :bleach @23:12:07.46 AS.seek 0.81ml @23:14:48.64 delay 3 seconds @23:14:51.67 ATV.dial :air3 @23:14:52.22 delay 3 seconds @23:14:55.31 AS.seek 0.835ml @23:15:00.72 delay 3 seconds @23:15:03.74 ATV.dial :bleach @23:15:04.37 AS.seek 0.82ml @23:15:07.78 delay 3 seconds @23:15:10.81 ATV.dial PCR @23:15:11.21 AS.reconfigure ASconfig @23:15:11.25 AS.seek :empty @23:15:58.70 PCR.program :bleachProfile @23:15:58.76 PCR program should complete in < 2:45 @23:15:58.77 PCR.run 10:00 @23:19:03.62 PCR.off @23:19:03.65 AFV.select :carrierSolution @23:19:03.75 delay 2 seconds @23:19:05.79 AS.seek 1ml @23:20:03.59 delay 1 second @23:20:04.61 AFV.select :holdingCoil @23:20:04.71 delay 1 second @23:20:05.75 AS.reconfigure ASfast @23:20:05.79 AS.seek 0ml @23:20:33.14 delay 3 seconds @23:20:36.17 AFV.select :carrierSolution @23:20:36.27 delay 2 seconds @23:20:38.31 AS.reconfigure ASconfig @23:20:38.36 AS.seek 1ml @23:21:36.16 delay 1 second @23:21:37.18 AFV.select :holdingCoil @23:21:37.28 delay 1 second @23:21:38.31 AS.reconfigure ASfast @23:21:38.36 AS.seek 0ml @23:22:05.72 delay 3 seconds @23:22:08.75 AFV.select :carrierSolution @23:22:08.85 delay 2 seconds @23:22:10.89 AS.reconfigure ASconfig @23:22:10.94 AS.seek 1ml @23:23:08.73 delay 1 second @23:23:09.75 AFV.select :holdingCoil @23:23:09.85 delay 1 second @23:23:10.89 AS.reconfigure ASfast @23:23:10.93 AS.seek 0ml @23:23:38.28 delay 3 seconds @23:23:41.30 AFV.select :carrierSolution @23:23:41.40 delay 2 seconds @23:23:43.44 AS.reconfigure ASconfig @23:23:43.49 AS.seek 1ml @23:24:41.28 delay 1 second @23:24:42.30 AFV.select :holdingCoil @23:24:42.40 delay 1 second @23:24:43.43 AS.reconfigure ASfast @23:24:43.48 AS.seek 0ml @23:25:10.84 delay 3 seconds @23:25:13.87 AFV.select :carrierSolution @23:25:13.97 delay 2 seconds @23:25:16.01 AS.reconfigure ASconfig @23:25:16.06 AS.seek 1ml @23:26:13.80 delay 1 second @23:26:14.82 AFV.select :holdingCoil @23:26:14.92 delay 1 second @23:26:15.96 AS.reconfigure ASnorm @23:26:16.01 AS.seek 0ml @23:27:06.74 Setting up PCR for Bb -- writing /var/log/esp/Bb18sep1223h1500ml.pcr @23:27:06.76 Building PCR Slug from ASV with MM3 mix, PP5 primer @23:27:06.77 Priming with carrier @23:27:06.78 ATV.dial ARV @23:27:07.37 ARV.dial :carrier @23:27:07.71 AS.seek 0.5ml @23:27:33.30 AFV.select :carrierSolution @23:27:33.41 delay 2 seconds @23:27:35.45 AS.reconfigure ASconfig @23:27:35.49 AS.seek 1ml @23:28:04.50 delay 1 second @23:28:05.52 AFV.select :holdingCoil @23:28:05.62 delay 1 second @23:28:06.65 ATV.dial :waste @23:28:08.31 AS.reconfigure ASnorm @23:28:08.36 AS.seek :empty @23:28:59.67 AFV.select :carrierSolution @23:28:59.78 delay 2 seconds @23:29:01.82 AS.reconfigure ASconfig @23:29:01.87 AS.seek 0.5ml @23:29:30.95 delay 1 second @23:29:31.97 AFV.select :holdingCoil @23:29:32.07 Building Slug @23:29:32.09 ATV.dial :ARV @23:29:33.80 delay 2 seconds @23:29:35.82 ARV.dial :air1 @23:29:36.32 delay 2 seconds @23:29:38.41 AS.reconfigure ASslow2 @23:29:38.47 AS.seek 0.506ml @23:29:44.94 delay 2 seconds @23:29:46.97 ARV.dial :MM3 @23:29:48.03 delay 2 seconds @23:29:50.06 Adding MM3 @23:29:50.14 AS.seek 0.524ml @23:30:08.42 delay 25 seconds @23:30:33.45 ARV.dial :PP5 @23:30:33.84 Adding PP5 @23:30:33.86 delay 2 seconds @23:30:35.94 AS.seek 0.53ml @23:30:42.33 delay 10 seconds @23:30:52.36 ARV.dial :air3 @23:30:52.75 adding ARV air @23:30:52.77 delay 2 seconds @23:30:54.85 AS.seek 0.536ml @23:31:01.22 delay 5 seconds @23:31:06.25 ARV.dial :carrier @23:31:07.53 delay 7 seconds @23:31:14.62 AS.seek 0.594ml @23:32:13.37 delay 5 seconds @23:32:18.40 ATV.dial :ASV @23:32:18.81 delay 2 seconds @23:32:20.90 AS.seek 0.6ml @23:32:27.19 delay 4 seconds @23:32:31.22 ATV.dial ARV @23:32:31.76 delay 2 seconds @23:32:33.79 ARV.dial :carrier @23:32:33.80 delay 2 seconds @23:32:35.88 AS.seek 0.65ml @23:33:26.26 delay 4 seconds @23:33:30.29 ATV.dial PCR @23:33:30.89 ASV.dial :waste1 @23:33:30.91 ARV.dial :air1 @23:33:31.32 Positioning Slug by 0.324ml @23:33:31.41 AS.seek 0.326ml @23:38:57.78 delay 5 seconds @23:39:02.81 ATV.dial ARV @23:39:03.58 ASV.dial :PCRlock @23:39:03.97 delay 5 seconds @23:39:09.00 Running Bb Profile60C300 thermocycling @23:39:09.02 PCR.program Profile60C300 @23:39:09.15 Profile60C300 should complete in < 1:45:21 @23:39:09.17 PCR.run 2:16:41.25 @01:04:01.88CEST13-Sep-18 PCR.off @01:04:03.47 PCR for Bb completed -- closed /var/log/esp/Bb18sep1223h1500ml.pcr @01:04:03.55 Cleaning PCR @01:04:03.56 AFV.select :carrierSolution @01:04:03.67 delay 2 seconds @01:04:05.71 AS.reconfigure ASconfig @01:04:05.75 AS.seek 0.5ml @01:04:16.04 delay 1 second @01:04:17.06 AFV.select :holdingCoil @01:04:17.16 ASV.dial :waste1 @01:04:17.54 ATV.dial :air3 @01:04:18.82 AS.reconfigure ASslow1 @01:04:18.87 AS.seek 0.51ml @01:04:21.18 delay 2 seconds @01:04:23.20 ATV.dial :bleach @01:04:23.82 AS.seek 0.81ml @01:05:24.42 delay 3 seconds @01:05:27.45 ATV.dial :air3 @01:05:28.00 delay 3 seconds @01:05:31.09 AS.seek 0.835ml @01:05:36.41 delay 3 seconds @01:05:39.43 ATV.dial :bleach @01:05:40.05 AS.seek 0.82ml @01:05:43.37 delay 3 seconds @01:05:46.39 ARV.dial :waste @01:05:47.01 delay 3 seconds @01:05:50.04 ATV.dial :ARV @01:05:50.93 AS.reconfigure ASconfig @01:05:50.98 AS.seek 0.47ml @01:06:11.39 AFV.select :carrierSolution @01:06:11.50 delay 2 seconds @01:06:13.54 AS.seek 1ml @01:06:44.31 delay 1 second @01:06:45.33 AFV.select :holdingCoil @01:06:45.43 delay 1 second @01:06:46.47 AS.reconfigure ASfast @01:06:46.51 AS.seek 0ml @01:07:13.86 AFV.select :carrierSolution @01:07:13.98 delay 2 seconds @01:07:16.02 AS.reconfigure ASconfig @01:07:16.06 AS.seek 1ml @01:08:13.88 delay 1 second @01:08:14.91 AFV.select :holdingCoil @01:08:15.01 delay 1 second @01:08:16.05 AS.reconfigure ASfast @01:08:16.09 AS.seek 0ml @01:08:43.45 AFV.select :carrierSolution @01:08:43.56 delay 2 seconds @01:08:45.60 AS.reconfigure ASconfig @01:08:45.65 AS.seek 1ml @01:09:43.45 delay 1 second @01:09:44.47 AFV.select :holdingCoil @01:09:44.57 delay 1 second @01:09:45.61 AS.reconfigure ASfast @01:09:45.65 AS.seek 0ml @01:10:13.01 ARV.dial between carrier and air1 @01:10:13.51 ATV.dial :air3 @01:10:14.80 AS.reconfigure ASslow1 @01:10:14.85 AS.seek 0.01ml @01:10:17.17 delay 2 seconds @01:10:19.19 ATV.dial :bleach @01:10:19.82 AS.seek 0.81ml @01:13:00.96 delay 3 seconds @01:13:03.99 ATV.dial :air3 @01:13:04.53 delay 3 seconds @01:13:07.62 AS.seek 0.835ml @01:13:12.84 delay 3 seconds @01:13:15.87 ATV.dial :bleach @01:13:16.49 AS.seek 0.82ml @01:13:19.90 delay 3 seconds @01:13:22.93 ATV.dial PCR @01:13:23.33 AS.reconfigure ASconfig @01:13:23.38 AS.seek :empty @01:14:10.84 PCR.program :bleachProfile @01:14:10.90 PCR program should complete in < 2:45 @01:14:10.91 PCR.run 10:00 @01:17:15.77 PCR.off @01:17:15.80 AFV.select :carrierSolution @01:17:15.91 delay 2 seconds @01:17:17.95 AS.seek 1ml @01:18:15.78 delay 1 second @01:18:16.80 AFV.select :holdingCoil @01:18:16.90 delay 1 second @01:18:17.94 AS.reconfigure ASfast @01:18:17.98 AS.seek 0ml @01:18:45.36 delay 3 seconds @01:18:48.39 AFV.select :carrierSolution @01:18:48.49 delay 2 seconds @01:18:50.53 AS.reconfigure ASconfig @01:18:50.59 AS.seek 1ml @01:19:48.42 delay 1 second @01:19:49.44 AFV.select :holdingCoil @01:19:49.54 delay 1 second @01:19:50.58 AS.reconfigure ASfast @01:19:50.62 AS.seek 0ml @01:20:18.00 delay 3 seconds @01:20:21.03 AFV.select :carrierSolution @01:20:21.13 delay 2 seconds @01:20:23.17 AS.reconfigure ASconfig @01:20:23.22 AS.seek 1ml @01:21:20.98 delay 1 second @01:21:22.00 AFV.select :holdingCoil @01:21:22.10 delay 1 second @01:21:23.14 AS.reconfigure ASfast @01:21:23.18 AS.seek 0ml @01:21:50.53 delay 3 seconds @01:21:53.56 AFV.select :carrierSolution @01:21:53.66 delay 2 seconds @01:21:55.70 AS.reconfigure ASconfig @01:21:55.74 AS.seek 1ml @01:22:53.48 delay 1 second @01:22:54.50 AFV.select :holdingCoil @01:22:54.60 delay 1 second @01:22:55.64 AS.reconfigure ASfast @01:22:55.68 AS.seek 0ml @01:23:23.04 delay 3 seconds @01:23:26.07 AFV.select :carrierSolution @01:23:26.17 delay 2 seconds @01:23:28.21 AS.reconfigure ASconfig @01:23:28.26 AS.seek 1ml @01:24:26.09 delay 1 second @01:24:27.11 AFV.select :holdingCoil @01:24:27.21 delay 1 second @01:24:28.25 AS.reconfigure ASnorm @01:24:28.29 AS.seek 0ml @01:25:19.03 Setting up PCR for IPc -- writing /var/log/esp/IPc18sep1301h1500ml.pcr @01:25:19.05 Building PCR Slug from ASV with MM1 mix, PP1 primer @01:25:19.06 Priming with carrier @01:25:19.07 ATV.dial ARV @01:25:19.67 ARV.dial :carrier @01:25:20.03 AS.seek 0.5ml @01:25:45.56 AFV.select :carrierSolution @01:25:45.67 delay 2 seconds @01:25:47.71 AS.reconfigure ASconfig @01:25:47.75 AS.seek 1ml @01:26:16.76 delay 1 second @01:26:17.78 AFV.select :holdingCoil @01:26:17.88 delay 1 second @01:26:18.91 ATV.dial :waste @01:26:20.55 AS.reconfigure ASnorm @01:26:20.60 AS.seek :empty @01:27:11.92 AFV.select :carrierSolution @01:27:12.03 delay 2 seconds @01:27:14.07 AS.reconfigure ASconfig @01:27:14.11 AS.seek 0.5ml @01:27:43.18 delay 1 second @01:27:44.20 AFV.select :holdingCoil @01:27:44.30 Building Slug @01:27:44.32 ATV.dial :ARV @01:27:46.01 delay 2 seconds @01:27:48.04 ARV.dial :air1 @01:27:48.44 delay 2 seconds @01:27:50.53 AS.reconfigure ASslow2 @01:27:50.59 AS.seek 0.506ml @01:27:56.90 delay 2 seconds @01:27:58.92 ARV.dial :MM1 @01:27:59.53 delay 2 seconds @01:28:01.56 Adding MM1 @01:28:01.63 AS.seek 0.524ml @01:28:19.87 delay 25 seconds @01:28:44.90 ARV.dial :PP1 @01:28:45.29 Adding PP1 @01:28:45.31 delay 2 seconds @01:28:47.40 AS.seek 0.53ml @01:28:53.64 delay 10 seconds @01:29:03.67 ARV.dial :air1 @01:29:04.06 adding ARV air @01:29:04.08 delay 2 seconds @01:29:06.16 AS.seek 0.536ml @01:29:12.34 delay 5 seconds @01:29:17.36 ARV.dial :carrier @01:29:17.76 delay 7 seconds @01:29:24.86 AS.seek 0.594ml @01:30:23.76 delay 5 seconds @01:30:28.79 ATV.dial :ASV @01:30:29.20 delay 2 seconds @01:30:31.29 AS.seek 0.6ml @01:30:37.64 delay 4 seconds @01:30:41.67 ATV.dial ARV @01:30:42.21 delay 2 seconds @01:30:44.24 ARV.dial :carrier @01:30:44.25 delay 2 seconds @01:30:46.34 AS.seek 0.65ml @01:31:37.01 delay 4 seconds @01:31:41.04 ATV.dial PCR @01:31:41.64 ASV.dial :waste1 @01:31:41.66 ARV.dial :air1 @01:31:42.07 Positioning Slug by 0.324ml @01:31:42.16 AS.seek 0.326ml @01:37:08.25 delay 5 seconds @01:37:13.28 ATV.dial ARV @01:37:14.04 ASV.dial :PCRlock @01:37:14.42 delay 5 seconds @01:37:19.45 Running IPc ProfileIPc thermocycling @01:37:19.47 PCR.program ProfileIPc @01:37:19.62 ProfileIPc should complete in < 1:50:49 @01:37:19.63 PCR.run 2:23:31.25 @03:04:36.60 PCR.off @03:04:39.25 PCR for IPc completed -- closed /var/log/esp/IPc18sep1301h1500ml.pcr @03:04:39.26 Full MFB Decontamination @03:04:39.28 Decon extraction column and decon AIV @03:04:39.30 SPE.hold 2:46:40,55C @03:04:39.89 ASV.dial :SPEdelivery @03:04:40.54 delay 20 seconds @03:05:00.58 AFV.select :carrierSolution @03:05:00.68 delay 2 seconds @03:05:02.71 AS.reconfigure ASconfig @03:05:02.76 AS.seek 0.5ml @03:05:13.09 delay 1 second @03:05:14.11 AFV.select :holdingCoil @03:05:14.21 ATV.dial :air1 @03:05:16.16 AS.reconfigure ASslow1 @03:05:16.21 AS.seek 0.52ml @03:05:20.51 ATV.dial :ASV @03:05:22.31 ASV.dial :SPErecovery @03:05:23.01 AS.seek 0.62ml @03:05:43.40 ATV.dial :waste @03:05:45.44 AS.reconfigure ASnorm @03:05:45.49 AS.seek :empty @03:06:17.13 AFV.select :holdingCoil @03:06:17.14 ATV.dial :waste @03:06:17.16 AS.reconfigure ASconfig @03:06:17.21 AS.seek 0ml @03:06:17.56 AFV.select :carrierSolution @03:06:17.67 delay 2 seconds @03:06:19.71 AS.seek 0.5ml @03:06:48.71 delay 1 second @03:06:49.74 AFV.select :holdingCoil @03:06:49.84 ATV.dial :air3 @03:06:50.56 AS.reconfigure ASslow1 @03:06:50.61 AS.seek 0.52ml @03:06:54.93 ATV.dial :air3 @03:06:55.02 AS.seek 0.53ml @03:06:57.34 delay 2 seconds @03:06:59.36 ATV.dial :bleach @03:06:59.84 AS.seek 0.71ml @03:07:36.32 delay 3 seconds @03:07:39.35 ATV.dial :air3 @03:07:39.93 delay 3 seconds @03:07:43.02 AS.seek 0.735ml @03:07:48.33 delay 3 seconds @03:07:51.35 ATV.dial :bleach @03:07:52.00 AS.seek 0.72ml @03:07:55.31 delay 3 seconds @03:07:58.33 ATV.dial ASV @03:07:59.40 ASV.dial :SPErecovery @03:07:59.49 AS.seek 0.365ml @03:09:11.15 ATV.dial :waste @03:09:12.89 AS.reconfigure ASnorm @03:09:12.94 AS.seek :empty @03:09:31.73 ATV.dial :air3 @03:09:32.50 AS.reconfigure ASslow1 @03:09:32.55 AS.seek 0.009ml @03:09:34.88 delay 2 seconds @03:09:36.90 ATV.dial :bleach @03:09:37.36 AS.seek 0.579ml @03:11:32.24 delay 3 seconds @03:11:35.26 ATV.dial :air3 @03:11:35.82 delay 3 seconds @03:11:38.91 AS.seek 0.604ml @03:11:44.14 delay 3 seconds @03:11:47.17 ATV.dial :bleach @03:11:47.80 AS.seek 0.589ml @03:11:51.10 delay 3 seconds @03:11:54.13 ATV.dial :ASV @03:11:55.15 ASV.dial :wasteR @03:11:55.55 AS.seek 0ml @03:13:54.32 delay 1:00 @03:14:54.35 ATV.dial ASV @03:14:54.36 AFV.select :carrierSolution @03:14:54.47 delay 2 seconds @03:14:56.51 AS.reconfigure ASconfig @03:14:56.55 AS.seek 1ml @03:15:54.32 delay 1 second @03:15:55.34 AFV.select :holdingCoil @03:15:55.44 delay 1 second @03:15:56.47 ASV.dial :wasteR @03:15:56.49 AS.reconfigure ASslow1 @03:15:56.53 AS.seek 0ml @03:19:17.91 delay 10 seconds @03:19:27.94 ATV.dial :air3 @03:19:29.44 AS.seek 0.01ml @03:19:31.77 delay 2 seconds @03:19:33.79 ATV.dial :bleach @03:19:34.41 AS.seek 0.58ml @03:21:29.32 delay 3 seconds @03:21:32.35 ATV.dial :air3 @03:21:32.90 delay 3 seconds @03:21:35.99 AS.seek 0.605ml @03:21:42.01 delay 3 seconds @03:21:45.04 ATV.dial :bleach @03:21:45.67 AS.seek 0.59ml @03:21:48.95 delay 3 seconds @03:21:51.97 ATV.dial ASV @03:21:52.99 ASV.dial :SPEdelivery @03:21:53.38 AS.seek 0ml @03:23:52.27 delay 1:00 @03:24:52.30 AFV.select :carrierSolution @03:24:52.40 delay 2 seconds @03:24:54.44 AS.reconfigure ASconfig @03:24:54.48 AS.seek 1ml @03:25:52.24 delay 1 second @03:25:53.27 AFV.select :holdingCoil @03:25:53.37 delay 1 second @03:25:54.40 ATV.dial ASV @03:25:54.41 ASV.dial :SPEdelivery @03:25:54.43 AS.reconfigure ASslow1 @03:25:54.48 AS.seek 0ml @03:29:15.87 delay 10 seconds @03:29:25.90 ATV.dial :air2 @03:29:26.74 AS.reconfigure ASslow0 @03:29:26.79 AS.seek 0.8ml @03:30:47.60 delay 3 seconds @03:30:50.62 ATV.dial :ASV @03:30:51.62 ASV.dial :SPEdelivery @03:30:51.64 AS.reconfigure ASslow1 @03:30:51.69 AS.seek 0.03ml @03:33:26.80 delay 20 seconds @03:33:46.83 ATV.dial :waste,via: :air3 @03:33:48.53 AS.reconfigure ASconfig @03:33:48.58 AS.seek :empty @03:33:50.65 SPE.coast @03:33:50.74 Removing Bleach from Mixing Coil @03:33:50.75 AFV.select :carrierSolution @03:33:50.86 delay 2 seconds @03:33:52.90 AS.seek 0.5ml @03:34:21.99 delay 1 second @03:34:23.01 AFV.select :holdingCoil @03:34:23.11 ATV.dial :ASV @03:34:24.76 ASV.dial :SPErecovery @03:34:25.41 AS.reconfigure ASslow1 @03:34:25.46 AS.seek 0.73ml @03:35:12.04 ASV.dial :wasteD @03:35:12.64 AS.reconfigure ASnorm @03:35:12.69 AS.seek 0ml @03:35:49.79 AFV.select :carrierSolution @03:35:49.90 delay 2 seconds @03:35:51.94 AS.reconfigure ASconfig @03:35:51.99 AS.seek 0.5ml @03:36:21.05 delay 1 second @03:36:22.07 AFV.select :holdingCoil @03:36:22.17 ATV.dial ASV @03:36:22.19 ASV.dial :SPErecovery @03:36:22.84 AS.reconfigure ASslow0 @03:36:22.90 AS.seek 0.3ml @03:36:43.37 ATV.dial :air2 @03:36:44.39 AS.reconfigure ASslow1 @03:36:44.44 AS.seek 0.32ml @03:36:48.88 ATV.dial ASV @03:36:49.91 AS.reconfigure ASslow0 @03:36:49.96 AS.seek 0.57ml @03:37:15.48 ASV.dial :wasteD @03:37:16.08 AS.reconfigure ASnorm @03:37:16.13 AS.seek 0ml @03:37:45.21 AFV.select :carrierSolution @03:37:45.32 delay 2 seconds @03:37:47.36 AS.reconfigure ASconfig @03:37:47.40 AS.seek 0.5ml @03:38:16.46 delay 1 second @03:38:17.49 AFV.select :holdingCoil @03:38:17.59 ATV.dial ASV @03:38:17.61 ASV.dial :SPErecovery @03:38:18.27 AS.reconfigure ASslow0 @03:38:18.32 AS.seek 0.3ml @03:38:38.79 ATV.dial :air2 @03:38:39.83 AS.reconfigure ASslow1 @03:38:39.88 AS.seek 0.32ml @03:38:44.25 ATV.dial ASV @03:38:45.28 AS.reconfigure ASslow0 @03:38:45.33 AS.seek 0.57ml @03:39:10.76 ASV.dial :wasteD @03:39:11.36 AS.reconfigure ASnorm @03:39:11.41 AS.seek 0ml @03:39:40.48 ATV.dial :air2 @03:39:41.44 AS.reconfigure ASslow0 @03:39:41.49 AS.seek 0.3ml @03:40:11.98 ATV.dial :ASV @03:40:12.95 ASV.dial :wasteD @03:40:12.97 AS.reconfigure ASconfig @03:40:13.60 AS.seek 0.05ml @03:40:28.27 ATV.dial :waste @03:40:30.08 AS.seek 0ml @03:40:33.26 ASV.dial :SPEdelivery @03:40:34.26 deconLysateINT @03:40:34.28 ATV.dial :air3 @03:40:34.95 AS.reconfigure ASslow1 @03:40:35.00 AS.seek 0.01ml @03:40:37.45 delay 2 seconds @03:40:39.48 ATV.dial :bleach @03:40:39.95 AS.seek 0.96ml @03:43:51.38 delay 3 seconds @03:43:54.41 ATV.dial :air3 @03:43:54.96 delay 3 seconds @03:43:58.05 AS.seek 0.985ml @03:44:03.36 delay 3 seconds @03:44:06.39 ATV.dial :bleach @03:44:07.03 AS.seek 0.97ml @03:44:10.40 delay 3 seconds @03:44:13.43 ATV.dial AIV @03:44:14.65 AIV.dial :coilToMFB @03:44:14.67 AWV.select :waste @03:44:14.68 AFV.select :carrierSolution @03:44:14.79 delay 2 seconds @03:44:16.83 AS.reconfigure ASconfig @03:44:16.87 AS.seek 1ml @03:44:18.88 delay 1 second @03:44:19.90 AFV.select :holdingCoil @03:44:20.01 delay 1 second @03:44:21.04 AS.reconfigure ASslow0 @03:44:21.09 AS.seek :empty @03:46:02.10 AFV.select :carrierSolution @03:46:02.21 delay 2 seconds @03:46:04.25 AS.reconfigure ASconfig @03:46:04.29 AS.seek 1ml @03:47:02.09 delay 1 second @03:47:03.11 AFV.select :holdingCoil @03:47:03.21 delay 1 second @03:47:04.24 AS.reconfigure ASslow0 @03:47:04.29 AS.seek :empty @03:48:45.29 AFV.select :carrierSolution @03:48:45.40 delay 2 seconds @03:48:47.44 AS.reconfigure ASconfig @03:48:47.48 AS.seek 1ml @03:49:45.29 delay 1 second @03:49:46.31 AFV.select :holdingCoil @03:49:46.41 delay 1 second @03:49:47.44 AS.reconfigure ASslow0 @03:49:47.49 AS.seek :empty @03:51:28.48 ATV.dial :air2 @03:51:29.16 AS.reconfigure ASslow1 @03:51:29.21 AS.seek 0.009ml @03:51:31.63 AS.reconfigure ASslow0 @03:51:31.68 AS.seek 1ml @03:53:11.65 ATV.dial AIV @03:53:12.42 AS.seek 0.02ml @03:54:51.34 ATV.dial :waste @03:54:52.95 AS.seek 0ml @03:54:55.27 AWV.select :air @03:54:55.44 Cleaning PCR @03:54:55.45 AFV.select :carrierSolution @03:54:55.55 delay 2 seconds @03:54:57.59 AS.reconfigure ASconfig @03:54:57.63 AS.seek 0.5ml @03:55:26.68 delay 1 second @03:55:27.70 AFV.select :holdingCoil @03:55:27.80 ASV.dial :waste1 @03:55:28.26 ATV.dial :air3 @03:55:28.94 AS.reconfigure ASslow1 @03:55:28.99 AS.seek 0.51ml @03:55:31.37 delay 2 seconds @03:55:33.39 ATV.dial :bleach @03:55:33.84 AS.seek 0.81ml @03:56:34.46 delay 3 seconds @03:56:37.49 ATV.dial :air3 @03:56:38.04 delay 3 seconds @03:56:41.13 AS.seek 0.835ml @03:56:46.55 delay 3 seconds @03:56:49.58 ATV.dial :bleach @03:56:50.20 AS.seek 0.82ml @03:56:53.49 delay 3 seconds @03:56:56.52 ARV.dial :waste @03:56:57.13 delay 3 seconds @03:57:00.15 ATV.dial :ARV @03:57:01.12 AS.reconfigure ASconfig @03:57:01.17 AS.seek 0.47ml @03:57:21.59 AFV.select :carrierSolution @03:57:21.70 delay 2 seconds @03:57:23.74 AS.seek 1ml @03:57:54.52 delay 1 second @03:57:55.54 AFV.select :holdingCoil @03:57:55.64 delay 1 second @03:57:56.68 AS.reconfigure ASfast @03:57:56.72 AS.seek 0ml @03:58:24.10 AFV.select :carrierSolution @03:58:24.78 delay 2 seconds @03:58:26.82 AS.reconfigure ASconfig @03:58:26.86 AS.seek 1ml @03:59:24.68 delay 1 second @03:59:25.70 AFV.select :holdingCoil @03:59:25.80 delay 1 second @03:59:26.84 AS.reconfigure ASfast @03:59:26.88 AS.seek 0ml @03:59:54.24 AFV.select :carrierSolution @03:59:54.35 delay 2 seconds @03:59:56.39 AS.reconfigure ASconfig @03:59:56.43 AS.seek 1ml @04:00:54.23 delay 1 second @04:00:55.25 AFV.select :holdingCoil @04:00:55.35 delay 1 second @04:00:56.39 AS.reconfigure ASfast @04:00:56.44 AS.seek 0ml @04:01:23.80 ARV.dial between carrier and air1 @04:01:24.30 ATV.dial :air3 @04:01:25.56 AS.reconfigure ASslow1 @04:01:25.61 AS.seek 0.01ml @04:01:27.98 delay 2 seconds @04:01:30.01 ATV.dial :bleach @04:01:30.65 AS.seek 0.81ml @04:04:11.79 delay 3 seconds @04:04:14.82 ATV.dial :air3 @04:04:15.37 delay 3 seconds @04:04:18.46 AS.seek 0.835ml @04:04:23.79 delay 3 seconds @04:04:26.82 ATV.dial :bleach @04:04:27.44 AS.seek 0.82ml @04:04:30.76 delay 3 seconds @04:04:33.78 ATV.dial PCR @04:04:34.17 AS.reconfigure ASconfig @04:04:34.22 AS.seek :empty @04:05:21.68 PCR.program :bleachProfile @04:05:21.74 PCR program should complete in < 2:45 @04:05:21.75 PCR.run 10:00 @04:08:35.69 PCR.off @04:08:35.72 AFV.select :carrierSolution @04:08:35.82 delay 2 seconds @04:08:37.86 AS.seek 1ml @04:09:35.68 delay 1 second @04:09:36.70 AFV.select :holdingCoil @04:09:36.80 delay 1 second @04:09:37.84 AS.reconfigure ASfast @04:09:37.88 AS.seek 0ml @04:10:05.24 delay 3 seconds @04:10:08.26 AFV.select :carrierSolution @04:10:08.36 delay 2 seconds @04:10:10.40 AS.reconfigure ASconfig @04:10:10.45 AS.seek 1ml @04:11:08.24 delay 1 second @04:11:09.26 AFV.select :holdingCoil @04:11:09.36 delay 1 second @04:11:10.39 AS.reconfigure ASfast @04:11:10.44 AS.seek 0ml @04:11:37.77 delay 3 seconds @04:11:40.79 AFV.select :carrierSolution @04:11:40.89 delay 2 seconds @04:11:42.93 AS.reconfigure ASconfig @04:11:42.98 AS.seek 1ml @04:12:40.77 delay 1 second @04:12:41.79 AFV.select :holdingCoil @04:12:41.89 delay 1 second @04:12:42.93 AS.reconfigure ASfast @04:12:42.97 AS.seek 0ml @04:13:10.30 delay 3 seconds @04:13:13.33 AFV.select :carrierSolution @04:13:13.43 delay 2 seconds @04:13:15.47 AS.reconfigure ASconfig @04:13:15.52 AS.seek 1ml @04:14:13.32 delay 1 second @04:14:14.34 AFV.select :holdingCoil @04:14:14.44 delay 1 second @04:14:15.48 AS.reconfigure ASfast @04:14:15.52 AS.seek 0ml @04:14:42.90 delay 3 seconds @04:14:45.93 AFV.select :carrierSolution @04:14:46.03 delay 2 seconds @04:14:48.07 AS.reconfigure ASconfig @04:14:48.12 AS.seek 1ml @04:15:45.90 delay 1 second @04:15:46.92 AFV.select :holdingCoil @04:15:47.02 delay 1 second @04:15:48.06 AS.reconfigure ASnorm @04:15:48.10 AS.seek 0ml @04:16:38.82 AFV.select :carrierSolution @04:16:38.92 delay 2 seconds @04:16:40.96 AS.reconfigure ASconfig @04:16:41.00 AS.seek 1ml @04:17:38.85 delay 1 second @04:17:39.87 AFV.select :holdingCoil @04:17:39.97 delay 1 second @04:17:40.99 ATV.dial :waste @04:17:42.19 AS.reconfigure ASnorm @04:17:42.24 AS.seek 0.5ml @04:18:07.80 ATV.dial :waste2 @04:18:09.05 AS.seek 0ml @04:18:35.17
Asleep after completing 18tun1 assays @04:18:35.18 Mission runtime was 13:35:38.53681 @04:18:35.21 GoodNews.email "{1 => 3, 2 => 19}\nCan@04:12:57, 22.6C, 46% humidity, 14.8psia, 12.011V, 0.682A, 0.682A avg, 8.20W",Subject: "Asleep after completing 18tun1 assays" @16:28:38.18CEST13-Sep-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @16:28:41.18 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @16:28:41.78 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @16:28:48.67 Polling Can every 10:00 @16:28:50.50 SPE.coast @16:28:50.56 SPE.configure SPEconfig @16:28:50.66 AS.reconfigure ASconfig @16:28:50.71 AS.hold @16:28:50.76 AS.configure ASconfig @16:28:50.86 SS.reconfigure SSconfig @16:28:50.91 SS.hold @16:28:50.97 SS.configure SSconfig @16:28:51.07 CC.reconfigure CCconfig @16:28:51.11 CC.hold @16:28:51.20 CC.configure CCconfig @16:28:51.29 CS.reconfigure CSconfig @16:28:51.35 CS.hold @16:28:51.41 CS.configure CSconfig @16:28:51.88 PC.reconfigure PCconfig @16:28:51.93 PC.hold @16:28:51.99 PC.configure PCconfig @16:28:52.08 PS.configure PSconfig @16:28:52.21 Forearm.reconfigure LooseForearm @16:28:52.25 Forearm.hold @16:28:52.31 Forearm.configure LooseForearm @16:28:52.41 Elbow.reconfigure LooseElbow @16:28:52.45 Elbow.hold @16:28:52.51 Elbow.configure LooseElbow @16:28:52.63 SC.reconfigure SCloose @16:28:52.68 SC.hold @16:28:52.76 SC.configure SCloose @16:28:52.86 SE.reconfigure SEconfig @16:28:52.91 SE.hold @16:28:52.98 SE.configure SEconfig @16:28:53.02 CH.coast @16:28:53.08 CH.configure CHconfig @16:28:53.11 PH.coast @16:28:53.18 PH.configure PHconfig @16:28:53.22 PCR.off @16:28:53.25 PCR.pidtemp= 50,0.1,0,300,25,25 @16:28:53.28 PCR.caltemp= 2.7073,0.0373,0 @16:28:53.31 All dwarf controllers running firmware version 3.75 @16:28:53.45 Cleanly exited at 16:21:21CEST13-Sep-18 @16:28:53.49 Loading /home/esp/esp2/mission/18tun1.rb mission @16:28:53.59 Preparing to sleep until +0:00:15 @16:28:53.64 GoodNews.email "Starting 18tun1 w/pucks in tube 2\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 4, 7 => 6}\nCan@16:28:48, 21.7C, 51% humidity, 14.8psia, 11.996V, 0.720A, 0.720A avg, 8.64W",Subject: "18tun1 begun" @16:28:53.79 AS.coast @16:28:53.84
Asleep til +0:00:15 @16:28:53.95 Continuing at 16:29 @16:28:54.03 SS.coast @16:28:54.15 CC.coast @16:28:54.23 CS.coast @16:28:54.27 PC.coast @16:28:54.31 Forearm.coast @16:28:54.36 Elbow.coast @16:28:54.40 SC.coast @16:28:54.44 SE.coast @16:28:54.84 Gate.power :core,:OFF @16:28:54.85 Gate.power :raw,:OFF @16:28:54.86 Shutdown @16:28:59.96 Session Begun @16:29:01.38 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:105\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 4, 7 => 6}\nCan@16:28:54, 21.7C, 51% humidity, 14.8psia, 11.992V, 0.720A, 0.733A avg, 8.64W",Subject: "Asleep until 16:29" @16:29:04.49 -> showlog 100 @16:29:08.54
Powering core bus @16:29:13.46 Gate.power :raw,:ON @16:29:14.19 SPE.coast @16:29:14.30 SPE.configure SPEconfig @16:29:14.42 AS.configure ASconfig @16:29:14.56 SS.configure SSconfig @16:29:14.68 CC.configure CCconfig @16:29:14.81 CS.configure CSconfig @16:29:14.95 PC.configure PCconfig @16:29:15.07 PS.configure PSconfig @16:29:15.25 Forearm.configure LooseForearm @16:29:15.37 Elbow.configure LooseElbow @16:29:15.53 SC.configure SCloose @16:29:15.64 SE.configure SEconfig @16:29:15.71 CH.coast @16:29:15.79 CH.configure CHconfig @16:29:15.84 PH.coast @16:29:15.92 PH.configure PHconfig @16:29:15.98 PCR.off @16:29:16.04 PCR.pidtemp= 50,0.1,0,300,25,25 @16:29:16.08 PCR.caltemp= 2.7073,0.0373,0 @16:29:16.13 All dwarf controllers running firmware version 3.75 @16:29:16.27 Valve.reset @16:29:16.47 PCR.off @16:29:16.51 PCR.pidtemp= 50,0.1,0,300,25,25 @16:29:16.56 PCR.caltemp= 2.7073,0.0373,0 @16:29:16.77 Intake.select :close @16:29:16.83 Exhaust.select :close @16:29:16.85 CTV.dial :air @16:29:16.98 PSV.dial :air @16:29:17.01 CSV.dial :waste1 @16:29:17.23 SSV.dial :spit,avoiding: :exhaust @16:29:17.69
Forearm.reconfigure LooseForearm @16:29:17.72 PS.reconfigure PSconfig @16:29:17.90
Forearm.home.seek :retract @16:29:17.92 PS.home.seek 0ml @16:29:18.14 CS.reconfigure CSfast @16:29:18.17 SS.reconfigure SSconfig @16:29:18.31 CS.home.seek 0ml @16:29:18.34 SS.home.seek 0ml @16:29:18.70 Hand.close @16:29:18.74
Elbow.jog 1500 @16:29:19.31 Elbow.reconfigure LooseElbow @16:29:19.38 Elbow.home.seek :clear @16:29:24.04 SE.reconfigure SEconfig @16:29:24.11 SE.home.seek :down @16:29:24.86 SC.reconfigure SCsloppy @16:29:24.93 SC.home.seek :initial @16:29:27.25 PS.coast @16:29:28.90
Forearm.seek :retract @16:29:29.01 Elbow.seek :hammer @16:29:30.19 Forearm.reconfigure TightForearm @16:29:30.27 Forearm.seek 5 + 350 counts @16:29:31.33 Forearm.seek :retract @16:29:32.50 PC.reconfigure PCconfig @16:29:32.53
CC.reconfigure CCconfig @16:29:32.62 PC.home.seek :open @16:29:32.69
CC.home.seek :open @16:29:36.06 Phase [mbaPhase] @16:29:36.08 GoodNews.email "At 16:29:35 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:105\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 4, 7 => 6}\nCan@16:29:36, 21.8C, 51% humidity, 14.8psia, 11.913V, 1.029A, 1.029A avg, 12.26W",Subject: "Started 18tun1 phase mbaPhase" @16:29:36.14CEST13-Sep-18
==================> STARTED MBAsuperphase (phase #1) <=== @16:29:36.24 Starting mfb @16:29:36.26 pcrBAC sampling at most 400ml, wcr at most 400ml @16:29:36.39 Loading sh1 puck from tube 2 into Collection Clamp @16:29:36.51 Readying the MFB @16:29:36.53
Hand.open @16:29:36.57 Homing MFB Syringe @16:29:36.62 AFV.select :holdingCoil @16:29:36.84 ATV.dial :air2 @16:29:37.59
SC.reconfigure SCloose @16:29:37.78 Elbow.reconfigure TightElbow @16:29:37.86 Elbow.seek 2 @16:29:38.41 AS.reconfigure ASconfig @16:29:38.53 AS.home.seek :home @16:29:38.67
Forearm.seek 2 + 700 counts @16:29:39.76 SE.reconfigure SEpick @16:29:39.86 SE.seek beamBreak @16:29:41.21 SE.configure SEconfig @16:29:41.42 Elbow.seek 2 @16:29:41.67 Forearm.seek 2 + 300 counts @16:29:42.14 Forearm.reconfigure LooseForearm @16:29:42.24 Hand.close @16:29:42.48 BACfans.email "Started mfb in tube 2 at 16:29:36CEST13-Sep-18\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 4, 7 => 6}\nCan@16:29:36, 21.8C, 51% humidity, 14.8psia, 11.913V, 1.029A, 1.029A avg, 12.26W",Subject: "pcrBAC sampling at most 400ml, wcr at most 400ml" @16:29:43.09 SE.seek :down @16:29:43.41
Forearm.seek :retract @16:29:44.09 AFV.select :carrierSolution @16:29:44.22 SE.coast @16:29:44.30 delay 2 seconds @16:29:44.41
Elbow.seek CC @16:29:46.04 Elbow.reconfigure LooseElbow @16:29:46.20 Forearm.reconfigure TightForearm @16:29:46.29 Forearm.seek CC @16:29:46.38 AS.seek 1ml @16:29:47.58
CC.seek :closed @16:29:51.92 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.50 @16:29:51.94 Hand.open @16:29:52.77 Forearm.reconfigure LooseForearm @16:29:52.84 Forearm.seek :retract @16:29:53.90 SC.coast @16:29:53.97 collectSample 400ml w/bubble point of 55psi @16:29:53.99 Priming 7ml @16:29:54.01 Intake.select :close @16:29:54.03 Exhaust.select :close @16:29:54.07 SS.hold @16:29:54.35 Calibrating pressure and temperature sensors @16:29:54.37 CTV.dial :air @16:29:54.40 SSV.dial :spit,avoiding: :exhaust @16:29:54.44 SS.reconfigure SSfast @16:29:54.53 SS.seek 5ml @16:29:57.42 SSV.dial CBV @16:29:57.45
CBV.dial SSV @16:29:59.02 delay 3.8 seconds @16:30:02.97 SSV.dial :spit @16:30:04.48 SS.seek 0ml @16:30:07.44 SSV.dial CBV @16:30:08.95 CTV.dial :intake @16:30:09.84 SSV.dial CBV @16:30:09.87
CBV.dial SSV @16:30:09.90 Allowing 40s for 7.0ml of remaining 7.0ml @16:30:09.94 Intake.select :open @16:30:12.15 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:30:12.17 SS.reconfigure SSinhale.with maxSpeed: 150 @16:30:12.26 SS.seek 7ml @16:30:17.73 Stroke completed -- waiting for pressure to equalize ... @16:30:37.35 Intake.select :close @16:30:37.53 SSV.dial :exhaust @16:30:38.41 Exhaust.select :open @16:30:38.56 SS.reconfigure SSexhale @16:30:38.63 SS.seek 0ml @16:30:39.28 delay 1 second @16:30:40.31 AFV.select :holdingCoil @16:30:40.45 delay 1 second @16:30:41.48 ATV.dial :waste @16:30:42.54 AS.reconfigure ASnorm @16:30:42.61 AS.home.seek :empty @16:30:43.01
Exhaust.select :close @16:30:43.14 Sampling 400.0ml @16:30:43.16 Intake.select :close @16:30:43.18 Exhaust.select :close @16:30:43.22 SS.reconfigure SSconfig @16:30:43.29 SS.hold @16:30:44.03 CTV.dial :intake @16:30:44.05 SSV.dial CBV @16:30:44.09
CBV.dial SSV @16:30:44.97 Allowing 130s for 25.0ml of remaining 400.0ml @16:30:44.99 Intake.select :open @16:30:47.19 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:30:47.22 SS.reconfigure SSinhale.with maxSpeed: 150 @16:30:47.31 SS.seek 25ml @16:30:50.35 Vacuum too high -- waiting up to 40s @16:30:55.31 Intake @16.0psia, vacuum from 9.5 to 13.5psi @16:30:55.34 SS.reconfigure SSinhale.with maxSpeed: 135 @16:30:55.43 SS.seek 25ml @16:30:57.78 Vacuum too high -- waiting up to 40s @16:31:01.71 Intake @15.9psia, vacuum from 9.4 to 13.4psi @16:31:01.76 SS.reconfigure SSinhale.with maxSpeed: 122 @16:31:01.90 SS.seek 25ml @16:31:04.68 Vacuum too high -- waiting up to 40s @16:31:09.56 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:31:09.59 SS.reconfigure SSinhale.with maxSpeed: 110 @16:31:09.68 SS.seek 25ml @16:31:12.16 Vacuum too high -- waiting up to 40s @16:31:16.58 SS.reconfigure SSinhale.with maxSpeed: 99 @16:31:16.66 SS.seek 25ml @16:31:19.91 Vacuum too high -- waiting up to 40s @16:31:24.31 Intake @15.9psia, vacuum from 9.4 to 13.4psi @16:31:24.33 SS.reconfigure SSinhale.with maxSpeed: 89 @16:31:24.42 SS.seek 25ml @16:31:27.57 Vacuum too high -- waiting up to 40s @16:31:31.98 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:31:32.00 SS.reconfigure SSinhale.with maxSpeed: 80 @16:31:32.09 SS.seek 25ml @16:31:35.25 ATV.dial :waste @16:31:35.27 ARV.dial between carrier and air1 @16:31:35.87 ASV.dial :waste1 @16:31:36.03
Vacuum too high -- waiting up to 40s @16:31:40.47 Intake @15.9psia, vacuum from 9.4 to 13.4psi @16:31:40.49 SS.reconfigure SSinhale.with maxSpeed: 72 @16:31:40.58 SS.seek 25ml @16:31:44.38 Vacuum too high -- waiting up to 40s @16:31:48.50 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:31:48.52 SS.reconfigure SSinhale.with maxSpeed: 65 @16:31:48.61 SS.seek 25ml @16:31:48.87 Stroke completed -- waiting for pressure to equalize ... @16:31:50.81 Intake.select :close @16:31:50.99 SSV.dial :exhaust @16:31:51.89 Exhaust.select :open @16:31:52.03 SS.reconfigure SSexhale @16:31:52.10 SS.seek 0ml @16:32:01.64 Exhaust.select :close @16:32:01.79 SSV.dial CBV @16:32:01.82
CBV.dial SSV @16:32:02.72 Allowing 130s for 25.0ml of remaining 375.0ml @16:32:02.74 Intake.select :open @16:32:04.95 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:32:04.97 SS.reconfigure SSinhale.with maxSpeed: 65 @16:32:05.06 SS.seek 25ml @16:32:10.59 Vacuum too high -- waiting up to 40s @16:32:14.93 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:32:14.95 SS.reconfigure SSinhale.with maxSpeed: 59 @16:32:15.04 SS.seek 25ml @16:32:19.16 Vacuum too high -- waiting up to 40s @16:32:22.56 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:32:22.59 SS.reconfigure SSinhale.with maxSpeed: 53 @16:32:22.68 SS.seek 25ml @16:32:28.57 Vacuum too high -- waiting up to 40s @16:32:32.73 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:32:32.76 SS.reconfigure SSinhale.with maxSpeed: 48 @16:32:32.85 SS.seek 25ml @16:32:41.00 Vacuum too high -- waiting up to 40s @16:32:45.12 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:32:45.14 SS.reconfigure SSinhale.with maxSpeed: 43 @16:32:45.23 SS.seek 25ml @16:32:57.38 Vacuum too high -- waiting up to 40s @16:33:01.77 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:33:01.80 SS.reconfigure SSinhale.with maxSpeed: 39 @16:33:01.89 SS.seek 25ml @16:33:03.24 Stroke completed -- waiting for pressure to equalize ... @16:33:05.83 Intake.select :close @16:33:06.01 SSV.dial :exhaust @16:33:06.89 Exhaust.select :open @16:33:07.03 SS.reconfigure SSexhale @16:33:07.10 SS.seek 0ml @16:33:16.78 Exhaust.select :close @16:33:16.93 SSV.dial CBV @16:33:16.96
CBV.dial SSV @16:33:17.84 Allowing 130s for 25.0ml of remaining 350.0ml @16:33:17.86 Intake.select :open @16:33:20.06 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:33:20.09 SS.reconfigure SSinhale.with maxSpeed: 39 @16:33:20.18 SS.seek 25ml @16:33:34.41 Vacuum too high -- waiting up to 40s @16:33:37.59 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:33:37.61 SS.reconfigure SSinhale.with maxSpeed: 35 @16:33:37.70 SS.seek 25ml @16:34:12.69 Stroke completed -- waiting for pressure to equalize ... @16:34:18.43 Intake.select :close @16:34:18.61 SSV.dial :exhaust @16:34:19.48 Exhaust.select :open @16:34:19.62 SS.reconfigure SSexhale @16:34:19.68 SS.seek 0ml @16:34:28.95 Exhaust.select :close @16:34:29.09 SSV.dial CBV @16:34:29.12
CBV.dial SSV @16:34:30.01 Allowing 130s for 25.0ml of remaining 325.0ml @16:34:30.02 Intake.select :open @16:34:32.23 Intake @15.9psia, vacuum from 9.4 to 13.4psi @16:34:32.25 SS.reconfigure SSinhale.with maxSpeed: 35 @16:34:32.35 SS.seek 25ml @16:34:40.58 -> exit @16:34:40.60 Session Ended @16:35:23.81
Stroke completed -- waiting for pressure to equalize ... @16:35:30.67 Intake.select :close @16:35:30.83 SSV.dial :exhaust @16:35:31.68 Exhaust.select :open @16:35:31.80 SS.reconfigure SSexhale @16:35:31.85 SS.seek 0ml @16:35:41.39 Exhaust.select :close @16:35:41.52 SSV.dial CBV @16:35:41.55
CBV.dial SSV @16:35:42.40 Allowing 130s for 25.0ml of remaining 300.0ml @16:35:42.41 Intake.select :open @16:35:44.58 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:35:44.60 SS.reconfigure SSinhale.with maxSpeed: 35 @16:35:44.65 SS.seek 25ml @16:36:35.64 Stroke completed -- waiting for pressure to equalize ... @16:36:42.03 Intake.select :close @16:36:42.18 SSV.dial :exhaust @16:36:43.04 Exhaust.select :open @16:36:43.16 SS.reconfigure SSexhale @16:36:43.21 SS.seek 0ml @16:36:52.90 Exhaust.select :close @16:36:53.02 SSV.dial CBV @16:36:53.04
CBV.dial SSV @16:36:53.93 Allowing 130s for 25.0ml of remaining 275.0ml @16:36:53.94 Intake.select :open @16:36:56.11 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:36:56.13 SS.reconfigure SSinhale.with maxSpeed: 35 @16:36:56.18 SS.seek 25ml @16:37:46.75 Stroke completed -- waiting for pressure to equalize ... @16:37:53.13 Intake.select :close @16:37:53.29 SSV.dial :exhaust @16:37:54.15 Exhaust.select :open @16:37:54.27 SS.reconfigure SSexhale @16:37:54.32 SS.seek 0ml @16:38:03.54 Exhaust.select :close @16:38:03.66 SSV.dial CBV @16:38:03.68
CBV.dial SSV @16:38:04.54 Allowing 130s for 25.0ml of remaining 250.0ml @16:38:04.55 Intake.select :open @16:38:06.72 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:38:06.74 SS.reconfigure SSinhale.with maxSpeed: 35 @16:38:06.79 SS.seek 25ml @16:38:58.34 Stroke completed -- waiting for pressure to equalize ... @16:39:05.32 Intake.select :close @16:39:05.48 SSV.dial :exhaust @16:39:06.34 Exhaust.select :open @16:39:06.46 SS.reconfigure SSexhale @16:39:06.50 SS.seek 0ml @16:39:15.71 Exhaust.select :close @16:39:15.83 SSV.dial CBV @16:39:16.24
CBV.dial SSV @16:39:17.20 Allowing 130s for 25.0ml of remaining 225.0ml @16:39:17.21 Intake.select :open @16:39:19.40 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:39:19.41 SS.reconfigure SSinhale.with maxSpeed: 35 @16:39:19.47 SS.seek 25ml @16:40:10.95 Stroke completed -- waiting for pressure to equalize ... @16:40:17.98 Intake.select :close @16:40:18.13 SSV.dial :exhaust @16:40:18.98 Exhaust.select :open @16:40:19.10 SS.reconfigure SSexhale @16:40:19.15 SS.seek 0ml @16:40:28.62 Exhaust.select :close @16:40:28.74 SSV.dial CBV @16:40:28.76
CBV.dial SSV @16:40:29.62 Allowing 130s for 25.0ml of remaining 200.0ml @16:40:29.63 Intake.select :open @16:40:31.80 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:40:31.82 SS.reconfigure SSinhale.with maxSpeed: 35 @16:40:31.87 SS.seek 25ml @16:41:22.62 Stroke completed -- waiting for pressure to equalize ... @16:41:29.01 Intake.select :close @16:41:29.16 SSV.dial :exhaust @16:41:30.01 Exhaust.select :open @16:41:30.13 SS.reconfigure SSexhale @16:41:30.17 SS.seek 0ml @16:41:39.90 Exhaust.select :close @16:41:40.02 SSV.dial CBV @16:41:40.05
CBV.dial SSV @16:41:40.90 Allowing 130s for 25.0ml of remaining 175.0ml @16:41:40.91 Intake.select :open @16:41:43.08 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:41:43.10 SS.reconfigure SSinhale.with maxSpeed: 35 @16:41:43.15 SS.seek 25ml @16:42:33.74 Stroke completed -- waiting for pressure to equalize ... @16:42:39.47 Intake.select :close @16:42:39.63 SSV.dial :exhaust @16:42:40.49 Exhaust.select :open @16:42:40.61 SS.reconfigure SSexhale @16:42:40.65 SS.seek 0ml @16:42:50.42 Exhaust.select :close @16:42:50.54 SSV.dial CBV @16:42:50.56
CBV.dial SSV @16:42:51.45 Allowing 130s for 25.0ml of remaining 150.0ml @16:42:51.46 Intake.select :open @16:42:53.63 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:42:53.65 SS.reconfigure SSinhale.with maxSpeed: 35 @16:42:53.70 SS.seek 25ml @16:43:44.18 Stroke completed -- waiting for pressure to equalize ... @16:43:49.77 Intake.select :close @16:43:49.93 SSV.dial :exhaust @16:43:50.81 Exhaust.select :open @16:43:50.93 SS.reconfigure SSexhale @16:43:50.98 SS.seek 0ml @16:44:00.51 Exhaust.select :close @16:44:00.63 SSV.dial CBV @16:44:00.65
CBV.dial SSV @16:44:01.51 Allowing 130s for 25.0ml of remaining 125.0ml @16:44:01.52 Intake.select :open @16:44:03.71 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:44:03.72 SS.reconfigure SSinhale.with maxSpeed: 35 @16:44:03.77 SS.seek 25ml @16:44:54.46 Stroke completed -- waiting for pressure to equalize ... @16:45:00.23 Intake.select :close @16:45:00.39 SSV.dial :exhaust @16:45:01.24 Exhaust.select :open @16:45:01.36 SS.reconfigure SSexhale @16:45:01.40 SS.seek 0ml @16:45:10.95 Exhaust.select :close @16:45:11.07 SSV.dial CBV @16:45:11.09
CBV.dial SSV @16:45:11.96 Allowing 130s for 25.0ml of remaining 100.0ml @16:45:11.97 Intake.select :open @16:45:14.15 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:45:14.16 SS.reconfigure SSinhale.with maxSpeed: 35 @16:45:14.21 SS.seek 25ml @16:46:04.96 Stroke completed -- waiting for pressure to equalize ... @16:46:11.37 Intake.select :close @16:46:11.53 SSV.dial :exhaust @16:46:12.40 Exhaust.select :open @16:46:12.52 SS.reconfigure SSexhale @16:46:12.56 SS.seek 0ml @16:46:22.10 Exhaust.select :close @16:46:22.22 SSV.dial CBV @16:46:22.24
CBV.dial SSV @16:46:23.10 Allowing 130s for 25.0ml of remaining 75.0ml @16:46:23.11 Intake.select :open @16:46:25.28 Intake @15.9psia, vacuum from 9.4 to 13.4psi @16:46:25.30 SS.reconfigure SSinhale.with maxSpeed: 35 @16:46:25.35 SS.seek 25ml @16:47:16.04 Stroke completed -- waiting for pressure to equalize ... @16:47:22.42 Intake.select :close @16:47:22.57 SSV.dial :exhaust @16:47:23.42 Exhaust.select :open @16:47:23.54 SS.reconfigure SSexhale @16:47:23.58 SS.seek 0ml @16:47:32.71 Exhaust.select :close @16:47:32.83 SSV.dial CBV @16:47:32.85
CBV.dial SSV @16:47:33.71 Allowing 130s for 25.0ml of remaining 50.0ml @16:47:33.72 Intake.select :open @16:47:35.90 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:47:35.91 SS.reconfigure SSinhale.with maxSpeed: 35 @16:47:35.96 SS.seek 25ml @16:48:27.45 Stroke completed -- waiting for pressure to equalize ... @16:48:34.29 Intake.select :close @16:48:34.45 SSV.dial :exhaust @16:48:35.31 Exhaust.select :open @16:48:35.43 SS.reconfigure SSexhale @16:48:35.47 SS.seek 0ml @16:48:44.66 Exhaust.select :close @16:48:44.78 SSV.dial CBV @16:48:44.80
CBV.dial SSV @16:48:45.66 Allowing 130s for 25.0ml of remaining 25.0ml @16:48:45.67 Intake.select :open @16:48:47.85 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:48:47.87 SS.reconfigure SSinhale.with maxSpeed: 35 @16:48:47.92 SS.seek 24.99ml @16:49:39.35 Stroke completed -- waiting for pressure to equalize ... @16:49:45.70 Intake.select :close @16:49:45.86 SSV.dial :exhaust @16:49:46.71 Exhaust.select :open @16:49:46.83 SS.reconfigure SSexhale @16:49:46.88 SS.seek 0ml @16:49:56.02 Exhaust.select :close @16:49:56.13 Sampled 400.0ml @16:49:56.15 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @16:49:56.16 Intake.select :close @16:49:56.22 CSV.dial :waste @16:49:56.31 CS.seek 0ml @16:49:56.39 SSV.dial CBV,avoiding: :exhaust @16:49:56.41
CBV.dial SSV @16:49:56.49 CSV.dial :air @16:49:57.32 CS.seek 10ml @16:49:58.54
CTV.dial :air @16:49:59.40 delay 15 seconds @16:50:14.43 SSV.dial :spit,avoiding: :exhaust @16:50:15.94 CTV.dial CSV,avoiding: :intake @16:50:15.98
SS.seek 0ml @16:50:16.12 SSV.dial CBV,avoiding: :exhaust @16:50:24.03 CBV.dial between SSV and waste2 @16:50:24.46 CTV.dial CSV,avoiding: :air @16:50:24.48
CSV.dial CTV @16:50:24.99 CS.reconfigure CSpressurize @16:50:25.04 CS.seek 0ml @16:50:51.21 CSV.dial :air @16:50:51.72 CS.reconfigure CSfast @16:50:51.76 CS.seek 10ml @16:51:18.05 CSV.dial between air and CTV @16:51:18.43 CS.seek 2.473ml @16:51:38.57 CTV.dial CSV,avoiding: :air @16:51:38.59
CSV.dial CTV @16:51:38.93 CS.reconfigure CSpressurize @16:51:38.97 CS.seek 0ml @16:51:42.98 BubblePoint: Intake @70.3psia, Outlet @15.3psia @16:51:42.99 CBV.dial SSV @16:51:43.36 SS.reconfigure SSconfig @16:51:43.41 SS.seek 10ml @16:51:49.10 delay 15 seconds @16:52:04.25 Evacuated: Intake @48.3psia, Outlet @4.5psia @16:52:04.26 SSV.dial :spit,avoiding: :exhaust @16:52:05.75 SS.reconfigure SSexhale @16:52:05.80 SS.seek 0ml @16:52:10.89 SS.reconfigure SSfast @16:52:10.94 SS.seek 5ml @16:52:13.83 SSV.dial CBV,avoiding: :exhaust @16:52:13.85
CBV.dial SSV @16:52:15.34 CTV.dial :air,avoiding: :intake @16:52:16.88 CSV.dial CTV @16:52:16.93
SS.seek 10ml @16:52:17.93 CSV.dial :air @16:52:18.44 CS.reconfigure CSfast @16:52:18.49 CS.seek 0ml @16:52:19.63
SSV.dial :spit,avoiding: :exhaust @16:52:21.15 SS.reconfigure SSexhale @16:52:21.20 SS.seek 0ml @16:52:22.87 CS.coast @16:52:26.37
SS.reconfigure SSfast @16:52:26.42 SS.seek 5ml @16:52:29.52 SSV.dial CBV,avoiding: :exhaust @16:52:29.55
CBV.dial SSV @16:52:32.06 SSV.dial :spit,avoiding: :exhaust @16:52:33.60 SS.reconfigure SSconfig @16:52:33.65 SS.seek 0ml @16:52:36.77 SS.coast @16:52:36.81 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @16:52:36.82 Intake.select :close @16:52:36.91 CSV.dial :waste @16:52:37.05 SSV.dial CBV,avoiding: :exhaust @16:52:37.16
CBV.dial SSV @16:52:37.79 CS.seek 0ml @16:52:37.86 CSV.dial :air @16:52:38.66
CTV.dial :air @16:52:38.68 delay 15 seconds @16:52:38.72 CS.seek 10ml @16:52:53.70
SSV.dial :spit,avoiding: :exhaust @16:52:55.19 CTV.dial CSV,avoiding: :intake @16:52:55.23
SS.reconfigure SSexhale @16:52:55.33 SS.seek 0ml @16:52:55.40 SSV.dial CBV,avoiding: :exhaust @16:53:05.16 CBV.dial between SSV and waste2 @16:53:05.60 CSV.dial between air and CTV @16:53:05.96 CS.seek 7.88ml @16:53:12.44 CTV.dial CSV,avoiding: :air @16:53:12.46
CSV.dial CTV @16:53:12.79 CS.reconfigure CSpressurize @16:53:12.84 CS.seek 0ml @16:53:33.56 CSV.dial :air @16:53:34.07 CS.reconfigure CSfast @16:53:34.13 CS.seek 10ml @16:54:00.53 CSV.dial between air and CTV @16:54:00.90 CS.seek 3.066ml @16:54:19.66 CTV.dial CSV,avoiding: :air @16:54:19.68
CSV.dial CTV @16:54:20.60 CS.reconfigure CSpressurize @16:54:20.72 CS.seek 0ml @16:54:28.44 BubblePoint: Intake @70.1psia, Outlet @14.9psia @16:54:28.45 CBV.dial SSV @16:54:28.82 SS.reconfigure SSconfig @16:54:28.87 SS.seek 10ml @16:54:34.46 delay 15 seconds @16:54:49.62 Evacuated: Intake @45.4psia, Outlet @14.0psia @16:54:49.63 SSV.dial :spit,avoiding: :exhaust @16:54:51.14 SS.reconfigure SSexhale @16:54:51.19 SS.seek 0ml @16:54:56.43 SS.reconfigure SSfast @16:54:56.47 SS.seek 5ml @16:54:59.52 SSV.dial CBV,avoiding: :exhaust @16:54:59.55
CBV.dial SSV @16:55:01.09 CTV.dial :air,avoiding: :intake @16:55:02.60 CSV.dial CTV @16:55:02.65
SS.seek 10ml @16:55:03.65 CSV.dial :air @16:55:04.15 CS.reconfigure CSfast @16:55:04.20 CS.seek 0ml @16:55:05.57
SSV.dial :spit,avoiding: :exhaust @16:55:06.10 CS.coast @16:55:07.08
SS.reconfigure SSexhale @16:55:07.13 SS.seek 0ml @16:55:12.56 SS.reconfigure SSfast @16:55:12.60 SS.seek 5ml @16:55:15.23 SSV.dial CBV,avoiding: :exhaust @16:55:15.25
CBV.dial SSV @16:55:17.77 SSV.dial :spit,avoiding: :exhaust @16:55:19.28 SS.reconfigure SSconfig @16:55:19.33 SS.seek 0ml @16:55:22.63 SS.coast @16:55:22.67 Lysing: Adding 1.5ml lysis @16:55:22.68 CBV.dial between CSV and waste1,avoiding: :DBV @16:55:24.37 CTV.dial CSV @16:55:25.87 CSV.dial CSR @16:55:26.77 Pulling 1ml of CSR.lysis @16:55:26.78 CSR.select :lysis @16:55:26.90 CS.reconfigure CSconfig @16:55:26.95 CS.seek 1ml @16:55:30.56 delay 5 seconds @16:55:35.59 CSR.select :air @16:55:35.76 CS.seek 6ml @16:55:49.50 delay 2 seconds @16:55:51.52 CSV.dial :waste1 @16:55:52.98 CS.reconfigure CSfast @16:55:53.03 CS.seek :empty @16:56:09.09 CSV.dial CSR @16:56:10.60 Pulling 1.5ml of CSR.lysis @16:56:10.61 CSR.select :lysis @16:56:10.74 CS.reconfigure CSconfig @16:56:10.79 CS.seek 1.5ml @16:56:15.65 delay 5 seconds @16:56:20.68 CSR.select :air @16:56:20.86 CS.seek 4.5ml @16:56:29.50 delay 2 seconds @16:56:31.52 CSV.dial CTV @16:56:32.04 CS.seek 1.275ml @16:56:41.26 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @16:56:41.28 CH.seek 85C,8:00 @16:59:42.88 CH.hold 5:00,85C @17:04:43.05 Collection Puck Heater at 85.1C after lysing @17:04:43.07 CS.seek 0ml @17:04:47.31 CTV.dial between spare and air,avoiding: :intake @17:04:48.49 CSV.dial :air @17:04:49.00 CS.seek 5ml @17:05:02.78 CSV.dial CTV @17:05:03.28 delay 2 seconds @17:05:05.31 CSV.dial :air @17:05:05.81 delay 2 seconds @17:05:07.83 CSV.dial CTV @17:05:08.35 delay 2 seconds @17:05:10.37 CSV.dial :air @17:05:10.88 delay 2 seconds @17:05:12.92 CS.seek 0ml @17:05:26.69 Recovering Lysate into Collection syringe @17:05:26.70 CSV.dial CBV @17:05:27.85 CBV.dial CSV @17:05:28.28 CS.seek 5ml @17:05:42.00 delay 5 seconds @17:05:47.03 CTV.dial :air @17:05:47.62 CS.seek 7ml @17:05:53.75 delay 5 seconds @17:05:58.77 CSV.dial :air2 @17:05:59.26 CS.seek 10ml @17:06:07.94 CSV.dial between air and CTV,avoiding: CBV @17:06:09.36 Lysing Complete @17:06:09.44 Storing sh1 puck in Collection Clamp to tube 1 @17:06:09.63 SC.reconfigure SCtight @17:06:09.69 SC.seek 1 @17:06:11.83 SC.reconfigure SCloose @17:06:12.06 Elbow.reconfigure TightElbow @17:06:12.11 Elbow.seek 1 @17:06:14.12 Forearm.reconfigure TightForearm @17:06:14.17 Forearm.seek 1 + 700 counts @17:06:15.23 SE.reconfigure SEpick @17:06:15.31 SE.seek beamBreak @17:06:18.33 SE.configure SEconfig @17:06:19.03 Elbow.reconfigure LimpElbow @17:06:19.08 Forearm.reconfigure LooseForearm @17:06:19.13 Forearm.seek 1 @17:06:19.44 Elbow.hold @17:06:19.52 Elbow.reconfigure TightElbow @17:06:19.63 SE.seek 2.07 Pucks below Top Plate @17:06:20.24 Forearm.force -20 @17:06:20.29 Forearm.seek 1 + 333 counts @17:06:20.61 Forearm.reconfigure TightForearm @17:06:20.66 Forearm.seek 1 + 700 counts @17:06:20.95 SE.seek 3.07 Pucks below Top Plate @17:06:21.56 Forearm.reconfigure LooseForearm @17:06:21.61 Forearm.seek :retract @17:06:22.53 Elbow.seek CC @17:06:24.21 Elbow.reconfigure LooseElbow @17:06:24.32 Forearm.seek CC @17:06:25.29 Hand.close @17:06:26.11 CC.seek :open @17:06:30.61 Forearm.seek :retract @17:06:31.60 Elbow.reconfigure TightElbow @17:06:31.65 Elbow.seek 1 @17:06:33.61 Elbow.reconfigure LimpElbow @17:06:33.67 Forearm.seek 1 @17:06:34.68 Elbow.hold @17:06:34.75 Elbow.reconfigure TightElbow @17:06:34.80 Hand.open @17:06:35.73 Forearm.force -20 @17:06:35.78 Forearm.seek 1 + 346 counts @17:06:36.11 Forearm.reconfigure TightForearm @17:06:36.15 Forearm.seek 1 + 700 counts @17:06:36.54 SE.seek :down @17:06:37.69
Forearm.reconfigure LooseForearm @17:06:37.74 Forearm.seek :retract @17:06:39.06 SE.coast @17:06:39.10
SC.coast @17:06:39.14 Tranferring lysate to PRV @17:06:39.15 CSV.dial :air @17:06:39.51 CS.seek 10ml @17:06:39.60 CSV.dial between air and CTV @17:06:39.96 AIV.dial :bypass @17:06:40.05 Beginning samplePassC @17:06:40.07 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:06:40.15 Hand.open @17:06:40.49 Elbow.seek FlushPuck::Garage @17:06:42.56 Elbow.reconfigure LooseElbow @17:06:42.66 Forearm.seek :garage @17:06:43.70 Hand.close @17:06:45.12 Forearm.seek :retract @17:06:46.16 Elbow.reconfigure TightElbow @17:06:46.21 Elbow.seek CC @17:06:49.23 Elbow.reconfigure LooseElbow @17:06:49.33 Forearm.reconfigure TightForearm @17:06:49.39 Forearm.seek CC @17:06:50.75 CC.seek :closed @17:06:54.99 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.33 @17:06:55.08 Hand.open @17:06:55.92 Forearm.reconfigure LooseForearm @17:06:55.97 Forearm.seek :retract @17:06:56.98 AWV.select :air @17:06:56.99 AIV.dial :bypass @17:06:57.01 PSV.dial PSR @17:06:58.54 Pulling 1.5ml of PSR.flush @17:06:58.55 PSR.select :flush @17:06:58.69 PS.seek 1.5ml @17:07:14.99 delay 5 seconds @17:07:20.02 PSV.dial :waste1 @17:07:20.56 PS.seek 0ml @17:07:36.89 PSV.dial PSR @17:07:37.39 PSR.select :air @17:07:37.51 PS.seek 2.5ml @17:08:04.21 PRV.dial CBV @17:08:04.25
CBV.dial PRV @17:08:05.11 PSV.dial :mixloop @17:08:06.39 CTV.dial :air,avoiding: :intake @17:08:06.40 PTV.dial :PRVmixing @17:08:06.49 PS.seek 0ml @17:08:33.14 PSV.dial :air @17:08:33.68 PS.seek 2.5ml @17:09:00.14 PSV.dial :mixloop @17:09:00.66 PS.seek 0ml @17:09:27.30 PSV.dial :air @17:09:27.81 PS.seek 2.5ml @17:09:54.23 PSV.dial :mixloop @17:09:54.73 PS.seek 0ml @17:10:21.01 Garaging FlushPuck in Collection Clamp @17:10:21.09 Elbow.reconfigure TightElbow @17:10:21.15 Elbow.seek CC @17:10:21.43 Elbow.reconfigure LooseElbow @17:10:21.54 Forearm.seek CC @17:10:22.56 Hand.close @17:10:23.36 CC.seek :open @17:10:27.76 Forearm.seek :retract @17:10:28.78 Elbow.reconfigure TightElbow @17:10:28.83 Elbow.seek :garage @17:10:31.80 Elbow.reconfigure LooseElbow @17:10:31.90 Forearm.reconfigure TightForearm @17:10:31.95 Forearm.seek :garage @17:10:33.15 Hand.open @17:10:34.36 Forearm.reconfigure LooseForearm @17:10:34.49 Forearm.seek :retract @17:10:35.53 Connecting PS to CS to transfer sample @17:10:35.54 CBV.dial AIV @17:10:35.56 AIV.dial :bypass @17:10:35.57 PSV.dial :mixloop @17:10:35.58 PTV.dial :PRVmixing @17:10:35.60 PRV.dial AIV @17:10:35.61 CTV.dial :air,avoiding: :intake @17:10:35.63 Loading lyfil puck from tube 2 into Collection Clamp @17:10:35.90 SC.reconfigure SCtight @17:10:35.95 SC.seek 2 @17:10:38.28 SC.reconfigure SCloose @17:10:38.52 Elbow.reconfigure TightElbow @17:10:38.57 Elbow.seek 2 @17:10:40.71 Forearm.reconfigure TightForearm @17:10:40.76 Forearm.seek 2 + 700 counts @17:10:41.79 SE.reconfigure SEpick @17:10:41.87 SE.seek beamBreak @17:10:43.36 SE.configure SEconfig @17:10:43.53 Elbow.seek 2 @17:10:43.74 Forearm.seek 2 + 300 counts @17:10:43.98 Forearm.reconfigure LooseForearm @17:10:44.03 Hand.close @17:10:44.75 SE.seek :down @17:10:44.99
Forearm.seek :retract @17:10:45.96 Elbow.seek CC @17:10:46.03 SE.coast @17:10:47.66
Elbow.reconfigure LooseElbow @17:10:47.77 Forearm.reconfigure TightForearm @17:10:47.82 Forearm.seek CC @17:10:49.10 CC.seek :closed @17:10:53.40 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.44 @17:10:53.41 Hand.open @17:10:54.18 Forearm.reconfigure LooseForearm @17:10:54.23 Forearm.seek :retract @17:10:55.23 SC.coast @17:10:55.27 CTV.dial CSV,avoiding: :intake @17:10:56.79 CSV.dial CTV @17:10:57.12 CS.seek 5ml @17:11:10.88 delay 1:20 @17:12:30.92 CS.seek 2.5ml @17:12:38.38 delay 40 seconds @17:13:18.42 CS.seek 0ml @17:13:25.85 delay 1:00 @17:14:25.88 PSV.dial :air @17:14:26.39 PS.seek 2.5ml @17:14:52.86 PSV.dial :mixloop @17:14:53.34 delay 3 seconds @17:14:56.36 PSV.dial :air @17:14:56.84 delay 2 seconds @17:14:58.86 PSV.dial :mixloop @17:14:59.34 delay 3 seconds @17:15:02.37 PSV.dial :waste1 @17:15:03.85 PS.seek :empty @17:15:30.02 CBV.dial :waste1 @17:15:30.54 delay 5 seconds @17:15:35.57 CBV.dial AIV @17:15:36.09 delay 10 seconds @17:15:46.59 CBV.dial :waste1 @17:15:47.15 delay 10 seconds @17:15:57.18 CBV.dial AIV @17:15:57.70 delay 10 seconds @17:16:07.73 CTV.dial CSV,avoiding: :intake @17:16:07.74 CBV.dial between AIV and waste1 @17:16:08.12 CSV.dial :air @17:16:08.64 CS.seek 10ml @17:16:35.23 CSV.dial CTV @17:16:35.75 CS.seek 0ml @17:17:02.32 CBV.dial :waste1 @17:17:02.65 delay 10 seconds @17:17:12.68 CTV.dial :air,avoiding: :intake @17:17:14.27 Storing lyfil puck in Collection Clamp to tube 1 @17:17:14.46 SC.reconfigure SCtight @17:17:14.51 SC.seek 1 @17:17:16.66 SC.reconfigure SCloose @17:17:16.90 Elbow.reconfigure TightElbow @17:17:16.95 Elbow.seek 1 @17:17:18.91 Forearm.reconfigure TightForearm @17:17:18.96 Forearm.seek 1 + 700 counts @17:17:20.15 SE.reconfigure SEpick @17:17:20.23 SE.seek beamBreak @17:17:23.18 SE.configure SEconfig @17:17:23.35 Elbow.reconfigure LimpElbow @17:17:23.40 Forearm.reconfigure LooseForearm @17:17:23.45 Forearm.seek 1 @17:17:23.75 Elbow.hold @17:17:23.84 Elbow.reconfigure TightElbow @17:17:23.95 SE.seek 3.08 Pucks below Top Plate @17:17:24.43 Forearm.force -20 @17:17:24.48 Forearm.seek 1 + 317 counts @17:17:24.83 Forearm.reconfigure TightForearm @17:17:24.88 Forearm.seek 1 + 700 counts @17:17:25.09 SE.seek 4.08 Pucks below Top Plate @17:17:25.69 Forearm.reconfigure LooseForearm @17:17:25.74 Forearm.seek :retract @17:17:26.69 Elbow.seek CC @17:17:28.40 Elbow.reconfigure LooseElbow @17:17:28.50 Forearm.seek CC @17:17:29.49 Hand.close @17:17:30.24 CC.seek :open @17:17:34.78 Forearm.seek :retract @17:17:35.77 Elbow.reconfigure TightElbow @17:17:35.82 Elbow.seek 1 @17:17:37.73 Elbow.reconfigure LimpElbow @17:17:37.78 Forearm.seek 1 @17:17:38.82 Elbow.hold @17:17:38.90 Elbow.reconfigure TightElbow @17:17:38.95 Hand.open @17:17:39.88 Forearm.force -20 @17:17:39.92 Forearm.seek 1 + 348 counts @17:17:40.26 Forearm.reconfigure TightForearm @17:17:40.79 Forearm.seek 1 + 700 counts @17:17:41.16 SE.seek :down @17:17:42.31
Forearm.reconfigure LooseForearm @17:17:42.36 Forearm.seek :retract @17:17:43.61 SE.coast @17:17:43.65
SC.coast @17:17:43.69 PSV.dial :air @17:17:44.93 PS.seek 0.499ml @17:17:50.80 delay 3 seconds @17:17:53.82 PSV.dial :mixloop @17:17:54.31 PRV.dial :air @17:17:54.81 delay 3 seconds @17:17:57.84 PRV.dial CBV @17:17:58.34 CBV.dial AIV @17:17:58.87 PRV.dial between CBV and air @17:17:59.22 PRV.dial :air @17:17:59.63 PS.reconfigure PSslow @17:17:59.68 PS.seek 0.599ml @17:18:02.19 PRV.dial :waste2 @17:18:03.04 PS.reconfigure PSconfig @17:18:03.09 PS.seek :empty @17:18:09.95 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:18:10.03 Hand.open @17:18:10.38 Elbow.seek FlushPuck::Garage @17:18:12.37 Elbow.reconfigure LooseElbow @17:18:12.47 Forearm.seek :garage @17:18:13.54 Hand.close @17:18:14.38 Forearm.seek :retract @17:18:15.38 Elbow.reconfigure TightElbow @17:18:15.43 Elbow.seek CC @17:18:18.28 Elbow.reconfigure LooseElbow @17:18:18.38 Forearm.reconfigure TightForearm @17:18:18.43 Forearm.seek CC @17:18:19.66 CC.seek :closed @17:18:23.91 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.33 @17:18:23.98 Hand.open @17:18:24.81 Forearm.reconfigure LooseForearm @17:18:24.87 Forearm.seek :retract @17:18:25.93 Clearing PML for modified lysate @17:18:25.94 CTV.dial :air,avoiding: :intake @17:18:25.95 PRV.dial :waste2 @17:18:25.97 PTV.dial :PRV,avoiding: :puck @17:18:26.80 delay 2 seconds @17:18:28.82 PTV.dial :PRVmixing,avoiding: :puck @17:18:29.64 delay 2 seconds @17:18:31.67 PSV.dial :PML @17:18:31.68 delay 2 seconds @17:18:33.70 PRV.dial :air @17:18:34.53 delay 2 seconds @17:18:36.62 PS.seek 1.499ml @17:18:52.86 delay 5 seconds @17:18:57.90 PS.seek 2.5ml @17:19:08.86 PSV.dial :air @17:19:09.88 PS.seek 0ml @17:19:36.36 mixing lysate with speDil @17:19:36.37 PSV.dial :PML @17:19:36.88 CBV.dial :PRV @17:19:36.89 PRV.dial :air @17:19:36.91 delay 2 seconds @17:19:38.93 PRV.dial :speDil @17:19:39.44 delay 2 seconds @17:19:41.54 PS.reconfigure PSslow1 @17:19:41.59 PS.seek 0.225ml @17:20:05.52 delay 5 seconds @17:20:10.55 PRV.dial CBV @17:20:11.39 delay 2 seconds @17:20:13.48 PS.seek 0.475ml @17:20:39.24 delay 5 seconds @17:20:44.27 PRV.dial :air @17:20:44.78 delay 2 seconds @17:20:46.82 PS.seek 0.965ml @17:21:36.86 delay 10 seconds @17:21:46.89 PRV.dial CBV @17:21:47.39 CTV.dial :air,avoiding: :intake @17:21:47.41 PSV.dial :air @17:21:47.92 PS.reconfigure PSconfig @17:21:47.97 PS.seek 2.5ml @17:22:04.30 delay 3 seconds @17:22:07.33 PSV.dial PTV @17:22:08.16 PTV.dial :PRV,avoiding: :puck @17:22:09.01 PS.seek 0ml @17:22:35.17 PSV.dial :air @17:22:36.00 PS.seek 2.5ml @17:23:02.27 delay 3 seconds @17:23:05.29 PSV.dial PTV @17:23:06.11 PTV.dial :PRV,avoiding: :puck @17:23:06.13 PS.seek 0ml @17:23:32.33 Pushing modified lysate into AIV Injection Loop @17:23:32.34 CBV.dial AIV @17:23:32.35 AIV.dial :bypass @17:23:32.37 PRV.dial CBV @17:23:32.38 PSV.dial :air @17:23:33.22 PS.seek 2.5ml @17:23:59.55 delay 3 seconds @17:24:02.57 PSV.dial :PML @17:24:03.07 PTV.dial :PRVmixing,avoiding: :puck @17:24:03.96 PS.reconfigure PSslow1 @17:24:04.01 PS.seek 1.74ml @17:25:21.77 delay 5 seconds @17:25:26.80 AIV.dial :sample @17:25:27.40 AWV.select :waste @17:25:27.57 PS.reconfigure PSslow2 @17:25:27.62 PS.seek 1.22ml @17:27:16.08 delay 15 seconds @17:27:31.11 AIV.dial :bypass @17:27:31.74 PS.reconfigure PSconfig @17:27:31.79 PS.seek 0ml @17:27:44.93 PSV.dial :air @17:27:45.44 PS.seek 2.5ml @17:28:11.77 PSV.dial :PML @17:28:12.26 PS.seek 0ml @17:28:38.43 AIV.dial :coilToMFB @17:28:38.44 AWV.select :air @17:28:38.61 Extracting Nucleic Acid from AIV @17:28:38.64
Beginning samplePassD @17:28:38.71 SPE.hold 2:46:40,55C @17:28:38.77 ASV.dial :SPEdelivery @17:28:38.85
AWV.select :air @17:28:38.86 AIV.dial :bypass @17:28:38.87 PSV.dial PSR @17:28:39.20 delay 20 seconds @17:28:40.17
Pulling 1.5ml of PSR.killMFB @17:28:40.18 PSR.select :killMFB @17:28:40.30 PS.seek 1.5ml @17:28:57.11 delay 5 seconds @17:28:59.23 primeLysateINT @17:28:59.24 AWV.select :air @17:28:59.26 AIV.dial :coilToMFB @17:28:59.27 ATV.dial AIV @17:29:01.16 AS.reconfigure ASslow1 @17:29:01.21 AS.seek 0.299ml @17:29:02.14
PSV.dial :waste1 @17:29:02.69 PS.seek 0ml @17:29:20.11 PSV.dial PSR @17:29:20.65 PSR.select :air @17:29:20.78 PS.seek 2.5ml @17:29:48.28 PRV.dial CBV @17:29:48.30
CBV.dial PRV @17:29:48.33 PSV.dial :mixloop @17:29:49.54 CTV.dial :air,avoiding: :intake @17:29:49.55 PTV.dial :PRVmixing @17:29:49.58 PS.seek 0ml @17:30:01.90 delay 10 seconds @17:30:11.93 ATV.dial :air2 @17:30:12.83 AS.seek 0.329ml @17:30:17.34
PSV.dial PSR @17:30:18.62 Pulling 1.5ml of PSR.flush @17:30:18.63 PSR.select :flush @17:30:18.77 PS.seek 1.5ml @17:30:19.23 ATV.dial :waste2 @17:30:19.85 AS.reconfigure ASnorm @17:30:19.90 AS.seek :empty @17:30:35.68
delay 5 seconds @17:30:36.89 Lysate primed! @17:30:36.90 AFV.select :holdingCoil @17:30:36.91 ATV.dial :air2 @17:30:37.57 AS.reconfigure ASslow1 @17:30:37.63 AS.seek 0.069ml @17:30:40.71
PSV.dial :waste1 @17:30:41.25 PS.seek 0ml @17:30:52.03 delay 3 seconds @17:30:55.06 ATV.dial :AIV @17:30:55.94 AS.seek 0.469ml @17:30:58.11
PSV.dial PSR @17:30:58.65 PSR.select :air @17:30:58.78 PS.seek 2.5ml @17:31:26.21 PRV.dial CBV @17:31:26.24
CBV.dial PRV @17:31:26.26 PSV.dial :mixloop @17:31:27.46 CTV.dial :air,avoiding: :intake @17:31:27.48 PTV.dial :PRVmixing @17:31:27.50 PS.seek 0ml @17:31:54.96 PSV.dial PSR @17:31:56.23 Pulling 1.5ml of PSR.flush @17:31:56.24 PSR.select :flush @17:31:56.37 PS.seek 1.5ml @17:32:13.02 delay 5 seconds @17:32:16.70 delay 5 seconds @17:32:18.05
PSV.dial :waste1 @17:32:18.58 PS.seek 0ml @17:32:21.77 Solid Phase Extraction Heater at 49.4C @17:32:21.78 ATV.dial ASV @17:32:22.18 ASV.dial :SPEdelivery @17:32:22.27 AS.seek 0.041ml @17:32:35.12
PSV.dial PSR @17:32:35.65 PSR.select :air @17:32:35.78 PS.seek 2.5ml @17:33:02.62 PRV.dial CBV @17:33:02.65
CBV.dial PRV @17:33:02.67 PSV.dial :mixloop @17:33:03.83 CTV.dial :air,avoiding: :intake @17:33:03.84 PTV.dial :PRVmixing @17:33:03.87 PS.seek 0ml @17:33:31.02 PSV.dial PSR @17:33:32.26 Pulling 1.5ml of PSR.flush @17:33:32.27 PSR.select :flush @17:33:32.40 PS.seek 1.5ml @17:33:48.64 delay 10 seconds @17:33:48.83
delay 5 seconds @17:33:53.86 PSV.dial :waste1 @17:33:54.39 PS.seek 0ml @17:33:58.66 rinseLysateLineINT @17:33:58.67 AFV.select :carrierSolution @17:33:58.78 delay 2 seconds @17:34:00.81 AS.reconfigure ASconfig @17:34:00.86 AS.seek 1ml @17:34:10.93
PSV.dial PSR @17:34:11.46 PSR.select :air @17:34:11.58 PS.seek 2.5ml @17:34:38.18 PRV.dial CBV @17:34:38.21
CBV.dial PRV @17:34:38.23 PSV.dial :mixloop @17:34:39.91 CTV.dial :air,avoiding: :intake @17:34:39.93 PTV.dial :PRVmixing @17:34:39.95 PS.reconfigure PSslow @17:34:40.00 PS.seek 1.2ml @17:34:56.26 delay 1 second @17:34:57.28 AFV.select :holdingCoil @17:34:57.38 delay 1 second @17:34:58.41 ATV.dial AIV @17:34:58.96 AIV.dial :coilToMFB @17:34:58.97 AWV.select :waste @17:34:59.09 AS.reconfigure ASslow0 @17:34:59.14 AS.seek 0ml @17:35:06.87
PSV.dial :waste1 @17:35:08.39 PS.reconfigure PSconfig @17:35:08.44 PS.seek 0ml @17:35:21.65 PSV.dial :air @17:35:22.83 PS.seek 2.5ml @17:35:49.18 PSV.dial :mixloop @17:35:49.69 PS.reconfigure PSslow @17:35:49.74 PS.seek 0ml @17:36:40.06 ATV.dial :air2 @17:36:40.74 AS.reconfigure ASslow1 @17:36:40.79 AS.seek 0.05ml @17:36:40.93
PSV.dial :air @17:36:41.44 PS.reconfigure PSconfig @17:36:41.50 PS.seek 2.5ml @17:36:51.17 ATV.dial :AIV @17:36:52.02 AS.seek 0.01ml @17:37:00.37 ATV.dial :waste @17:37:02.02 AS.seek :empty @17:37:04.45 ethanol wash @17:37:04.46 ATV.dial :air2 @17:37:05.73 AS.reconfigure ASslow2 @17:37:05.78 AS.seek 0.009ml @17:37:08.18
PSV.dial :mixloop @17:37:08.70 PS.reconfigure PSslow @17:37:08.75 PS.seek 0ml @17:37:16.15 delay 3 seconds @17:37:19.18 Pulling 0.4ml of Ethanol Wash @17:37:19.19 ATV.dial :ethanol @17:37:20.04 AS.reconfigure ASslow1 @17:37:20.10 AS.seek 0.409ml @17:37:59.90
Cleaning Collection Syringe @17:37:59.91 CSV.dial CSR @17:38:00.48 Pulling 4ml of CSR.flush @17:38:00.49 CSR.select :flush @17:38:00.62 CS.seek 4ml @17:38:12.08 delay 5 seconds @17:38:17.11 CSR.select :air @17:38:17.24 CS.seek 10ml @17:38:33.65 CSV.dial CTV @17:38:33.69
CTV.dial CSV @17:38:35.25 CBV.dial :waste1 @17:38:35.78 CS.seek 0ml @17:38:40.87 delay 3 seconds @17:38:43.90 ATV.dial :air1 @17:38:44.28 delay 3 seconds @17:38:47.37 AS.seek 0.434ml @17:38:52.70 delay 3 seconds @17:38:55.73 ATV.dial :ethanol @17:38:56.35 AS.seek 0.419ml @17:38:59.65 delay 3 seconds @17:39:02.30
CSV.dial CSR @17:39:02.67 ATV.dial ASV @17:39:02.98
Pulling 4ml of CSR.flush @17:39:02.99 CSR.select :flush @17:39:03.12 CS.seek 4ml @17:39:04.00 AS.seek 0.092ml @17:39:14.38
delay 5 seconds @17:39:19.40 CSR.select :air @17:39:19.53 CS.seek 10ml @17:39:35.93 CSV.dial CTV @17:39:35.97
CTV.dial CSV @17:39:36.47 CBV.dial :waste1 @17:39:36.50 CS.seek 0ml @17:40:03.09 Garaging FlushPuck in Collection Clamp @17:40:03.17 Elbow.reconfigure TightElbow @17:40:03.73 Elbow.seek CC @17:40:04.01 Elbow.reconfigure LooseElbow @17:40:04.12 Forearm.seek CC @17:40:05.09 Hand.close @17:40:05.90 CC.seek :open @17:40:10.03 delay 3 seconds @17:40:10.32
Forearm.seek :retract @17:40:11.30 Elbow.reconfigure TightElbow @17:40:11.35 Elbow.seek :garage @17:40:13.06 ATV.dial :waste2 @17:40:13.83 AS.reconfigure ASconfig @17:40:13.88 AS.seek :empty @17:40:14.41
Elbow.reconfigure LooseElbow @17:40:14.52 Forearm.reconfigure TightForearm @17:40:14.57 Forearm.seek :garage @17:40:15.73 Hand.open @17:40:16.58 Forearm.reconfigure LooseForearm @17:40:16.63 Forearm.seek :retract @17:40:17.69 Whole Cell Archive sampling 400ml @17:40:17.71 Loading wcr puck from tube 2 into Collection Clamp @17:40:17.98 SC.reconfigure SCtight @17:40:18.03 SC.seek 2 @17:40:19.53 ATV.dial :air2 @17:40:19.93 AS.reconfigure ASslow1 @17:40:19.98 AS.seek 1ml @17:40:20.22 SC.reconfigure SCloose @17:40:20.46 Elbow.reconfigure TightElbow @17:40:20.51 Elbow.seek 2 @17:40:22.76 Forearm.reconfigure TightForearm @17:40:22.82 Forearm.seek 2 + 700 counts @17:40:23.88 SE.reconfigure SEpick @17:40:23.97 SE.seek beamBreak @17:40:25.56 SE.configure SEconfig @17:40:25.75 Elbow.seek 2 @17:40:25.97 Forearm.seek 2 + 300 counts @17:40:26.15 Forearm.reconfigure LooseForearm @17:40:26.20 Hand.close @17:40:27.03 SE.seek :down @17:40:27.26 Forearm.seek :retract @17:40:28.22 Elbow.seek CC @17:40:28.32 SE.coast @17:40:29.91 Elbow.reconfigure LooseElbow @17:40:30.02 Forearm.reconfigure TightForearm @17:40:30.07 Forearm.seek CC @17:40:31.43 CC.seek :closed @17:40:35.73 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.50 @17:40:35.75 Hand.open @17:40:36.58 Forearm.reconfigure LooseForearm @17:40:36.64 Forearm.seek :retract @17:40:37.67 SC.coast @17:40:37.72 collectSample 400ml w/bubble point of 55psi @17:40:37.73 Priming 7ml @17:40:37.74 Intake.select :close @17:40:37.75 Exhaust.select :close @17:40:37.77 SS.hold @17:40:38.00 Calibrating pressure sensors @17:40:38.01 CTV.dial :air @17:40:39.52 SSV.dial :spit,avoiding: :exhaust @17:40:39.55 SS.reconfigure SSfast @17:40:39.60 SS.seek 5ml @17:40:42.39 SSV.dial CBV @17:40:42.42 CBV.dial SSV @17:40:44.01 delay 3.8 seconds @17:40:47.97 SSV.dial :spit @17:40:49.46 SS.seek 0ml @17:40:52.54 SSV.dial CBV @17:40:54.04 CTV.dial :intake @17:40:54.91 SSV.dial CBV @17:40:54.93 CBV.dial SSV @17:40:54.96 Allowing 40s for 7.0ml of remaining 7.0ml @17:40:54.97 Intake.select :open @17:40:57.15 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:40:57.17 SS.reconfigure SSinhale.with maxSpeed: 150 @17:40:57.22 SS.seek 7ml @17:41:02.41 Stroke completed -- waiting for pressure to equalize ... @17:41:18.87 Intake.select :close @17:41:19.03 SSV.dial :exhaust @17:41:19.90 Exhaust.select :open @17:41:20.02 SS.reconfigure SSexhale @17:41:20.07 SS.seek 0ml @17:41:24.19 Exhaust.select :close @17:41:24.30 Sampling 400.0ml @17:41:24.32 Intake.select :close @17:41:24.33 Exhaust.select :close @17:41:24.35 SS.reconfigure SSconfig @17:41:24.39 SS.hold @17:41:25.16 CTV.dial :intake @17:41:25.18 SSV.dial CBV @17:41:25.20 CBV.dial SSV @17:41:26.09 Allowing 130s for 25.0ml of remaining 400.0ml @17:41:26.11 Intake.select :open @17:41:28.29 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:41:28.31 SS.reconfigure SSinhale.with maxSpeed: 150 @17:41:28.36 SS.seek 25ml @17:41:33.23 Vacuum too high -- waiting up to 40s @17:41:45.45 Intake @16.0psia, vacuum from 9.5 to 13.5psi @17:41:45.48 SS.reconfigure SSinhale.with maxSpeed: 135 @17:41:45.52 SS.seek 25ml @17:41:49.54 Vacuum too high -- waiting up to 40s @17:42:02.51 Intake @16.0psia, vacuum from 9.5 to 13.5psi @17:42:02.53 SS.reconfigure SSinhale.with maxSpeed: 122 @17:42:02.58 SS.seek 25ml @17:42:06.64 Vacuum too high -- waiting up to 40s @17:42:20.42 Intake @16.0psia, vacuum from 9.5 to 13.5psi @17:42:20.44 SS.reconfigure SSinhale.with maxSpeed: 110 @17:42:20.50 SS.seek 25ml @17:42:25.29 Vacuum too high -- waiting up to 40s @17:42:39.85 Intake @16.1psia, vacuum from 9.6 to 13.6psi @17:42:39.87 SS.reconfigure SSinhale.with maxSpeed: 99 @17:42:39.92 SS.seek 25ml @17:42:40.47 Stroke completed -- waiting for pressure to equalize ... @17:42:46.88 Intake.select :close @17:42:47.04 SSV.dial :exhaust @17:42:47.91 Exhaust.select :open @17:42:48.03 SS.reconfigure SSexhale @17:42:48.08 SS.seek 0ml @17:42:57.62 Exhaust.select :close @17:42:57.73 SSV.dial CBV @17:42:57.77 CBV.dial SSV @17:42:58.65 Allowing 130s for 25.0ml of remaining 375.0ml @17:42:58.67 Intake.select :open @17:43:00.85 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:43:01.33 SS.reconfigure SSinhale.with maxSpeed: 99 @17:43:01.47 SS.seek 25ml @17:43:05.82 Vacuum too high -- waiting up to 40s @17:43:13.89 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:43:13.91 SS.reconfigure SSinhale.with maxSpeed: 89 @17:43:13.96 SS.seek 25ml @17:43:18.44 Vacuum too high -- waiting up to 40s @17:43:28.16 Intake @15.9psia, vacuum from 9.4 to 13.4psi @17:43:28.18 SS.reconfigure SSinhale.with maxSpeed: 80 @17:43:28.22 SS.seek 25ml @17:43:32.64 Vacuum too high -- waiting up to 40s @17:43:41.51 delay 5 seconds @17:43:42.50 Intake @15.9psia, vacuum from 9.4 to 13.4psi @17:43:42.52 SS.reconfigure SSinhale.with maxSpeed: 72 @17:43:42.57 SS.seek 25ml @17:43:46.53 ATV.dial ASV @17:43:47.53 ASV.dial :SPEdelivery @17:43:47.56 AS.seek 0.02ml @17:43:47.86 Vacuum too high -- waiting up to 40s @17:43:58.61 Intake @15.9psia, vacuum from 9.4 to 13.4psi @17:43:58.63 SS.reconfigure SSinhale.with maxSpeed: 65 @17:43:58.68 SS.seek 25ml @17:44:05.32 Vacuum too high -- waiting up to 40s @17:44:17.27 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:44:17.29 SS.reconfigure SSinhale.with maxSpeed: 59 @17:44:17.33 SS.seek 25ml @17:44:18.94 Stroke completed -- waiting for pressure to equalize ... @17:44:26.08 Intake.select :close @17:44:26.24 SSV.dial :exhaust @17:44:27.12 Exhaust.select :open @17:44:27.24 SS.reconfigure SSexhale @17:44:27.29 SS.seek 0ml @17:44:36.52 Exhaust.select :close @17:44:36.64 SSV.dial CBV @17:44:36.66 CBV.dial SSV @17:44:37.54 Allowing 130s for 25.0ml of remaining 350.0ml @17:44:37.56 Intake.select :open @17:44:39.74 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:44:39.76 SS.reconfigure SSinhale.with maxSpeed: 59 @17:44:39.81 SS.seek 25ml @17:44:48.01 Vacuum too high -- waiting up to 40s @17:44:56.81 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:44:56.83 SS.reconfigure SSinhale.with maxSpeed: 53 @17:44:56.88 SS.seek 25ml @17:45:03.95 Vacuum too high -- waiting up to 40s @17:45:13.59 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:45:13.61 SS.reconfigure SSinhale.with maxSpeed: 48 @17:45:13.66 SS.seek 25ml @17:45:24.23 Vacuum too high -- waiting up to 40s @17:45:35.94 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:45:35.96 SS.reconfigure SSinhale.with maxSpeed: 43 @17:45:36.00 SS.seek 25ml @17:45:47.66 Vacuum too high -- waiting up to 40s @17:45:59.16 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:45:59.18 SS.reconfigure SSinhale.with maxSpeed: 39 @17:45:59.23 SS.seek 25ml @17:46:00.45 Stroke completed -- waiting for pressure to equalize ... @17:46:06.88 Intake.select :close @17:46:07.04 SSV.dial :exhaust @17:46:07.92 Exhaust.select :open @17:46:08.04 SS.reconfigure SSexhale @17:46:08.09 SS.seek 0ml @17:46:17.79 Exhaust.select :close @17:46:17.91 SSV.dial CBV @17:46:17.93 CBV.dial SSV @17:46:18.82 Allowing 130s for 25.0ml of remaining 325.0ml @17:46:18.84 Intake.select :open @17:46:21.01 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:46:21.03 SS.reconfigure SSinhale.with maxSpeed: 39 @17:46:21.08 SS.seek 25ml @17:46:32.81 Vacuum too high -- waiting up to 40s @17:46:40.47 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:46:40.49 SS.reconfigure SSinhale.with maxSpeed: 35 @17:46:40.53 SS.seek 25ml @17:46:54.64 Vacuum too high -- waiting up to 40s @17:47:03.74 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:47:03.76 SS.reconfigure SSinhale.with maxSpeed: 32 @17:47:03.81 SS.seek 25ml @17:47:05.00 delay 5 seconds @17:47:10.02 ATV.dial :air2 @17:47:11.11 AS.seek 0.52ml @17:47:26.32 Vacuum too high -- waiting up to 40s @17:47:38.02 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:47:38.04 SS.reconfigure SSinhale.with maxSpeed: 29 @17:47:38.08 SS.seek 25ml @17:47:42.43 Stroke completed -- waiting for pressure to equalize ... @17:47:50.10 Intake.select :close @17:47:50.26 SSV.dial :exhaust @17:47:51.11 Exhaust.select :open @17:47:51.23 SS.reconfigure SSexhale @17:47:51.28 SS.seek 0ml @17:48:00.85 Exhaust.select :close @17:48:00.96 SSV.dial CBV @17:48:01.00 CBV.dial SSV @17:48:01.86 Allowing 130s for 25.0ml of remaining 300.0ml @17:48:01.88 Intake.select :open @17:48:04.05 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:48:04.07 SS.reconfigure SSinhale.with maxSpeed: 29 @17:48:04.12 SS.seek 25ml @17:48:33.51 Vacuum too high -- waiting up to 40s @17:48:43.72 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:48:43.74 SS.reconfigure SSinhale.with maxSpeed: 26 @17:48:43.79 SS.seek 25ml @17:48:52.04 delay 5 seconds @17:48:57.07 ATV.dial ASV @17:48:58.53 ASV.dial :wasteR @17:48:59.02 AS.seek 0.3ml @17:49:19.24 Stroke completed -- waiting for pressure to equalize ... @17:49:35.19 Intake.select :close @17:49:35.35 SSV.dial :exhaust @17:49:36.22 Exhaust.select :open @17:49:36.35 SS.reconfigure SSexhale @17:49:36.40 SS.seek 0ml @17:49:43.48 delay 20 seconds @17:49:46.07 Exhaust.select :close @17:49:46.19 SSV.dial CBV @17:49:46.22 CBV.dial SSV @17:49:47.10 Allowing 130s for 25.0ml of remaining 275.0ml @17:49:47.12 Intake.select :open @17:49:49.31 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:49:49.33 SS.reconfigure SSinhale.with maxSpeed: 26 @17:49:49.38 SS.seek 25ml @17:50:03.51 AS.seek 0.07ml @17:50:40.48 Vacuum too high -- waiting up to 40s @17:50:49.99 delay 20 seconds @17:50:52.48 SS.reconfigure SSinhale.with maxSpeed: 23 @17:50:52.54 SS.seek 25ml @17:51:10.03 ATV.dial :waste2 @17:51:10.81 AS.reconfigure ASconfig @17:51:10.86 AS.seek :empty @17:51:11.57 Stroke completed -- waiting for pressure to equalize ... @17:51:15.21 Elution @17:51:15.23 ATV.dial :air1 @17:51:16.35 AS.reconfigure ASslow1 @17:51:16.41 AS.seek 0.049ml @17:51:22.45 Intake.select :close @17:51:22.61 SSV.dial :exhaust @17:51:23.47 Exhaust.select :open @17:51:23.59 SS.reconfigure SSexhale @17:51:23.64 SS.seek 0ml @17:51:26.82 delay 3 seconds @17:51:29.85 ATV.dial :ElutionWater @17:51:30.53 AS.seek 0.109ml @17:51:33.24 Exhaust.select :close @17:51:33.36 SSV.dial CBV @17:51:33.38 CBV.dial SSV @17:51:34.25 Allowing 130s for 25.0ml of remaining 250.0ml @17:51:34.27 Intake.select :open @17:51:36.45 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:51:36.47 SS.reconfigure SSinhale.with maxSpeed: 23 @17:51:36.53 SS.seek 25ml @17:51:42.89 delay 3 seconds @17:51:45.92 ATV.dial ASV,via: :air2 @17:51:48.07 ASV.dial :SPEelution @17:51:48.68 AFV.select :carrierSolution @17:51:48.85 AS.reconfigure ASnorm @17:51:48.90 AS.seek 0.759ml @17:52:21.95 delay 3 seconds @17:52:24.98 AFV.select :holdingCoil @17:52:25.14 AS.reconfigure ASslow1 @17:52:25.19 AS.seek 0.474ml @17:52:53.83 Stroke completed -- waiting for pressure to equalize ... @17:53:11.00 Intake.select :close @17:53:11.16 SSV.dial :exhaust @17:53:12.03 Exhaust.select :open @17:53:12.15 SS.reconfigure SSexhale @17:53:12.20 SS.seek 0ml @17:53:21.82 Exhaust.select :close @17:53:21.94 SSV.dial CBV @17:53:21.96 CBV.dial SSV @17:53:22.79 delay 5 seconds @17:53:22.85 Allowing 130s for 25.0ml of remaining 225.0ml @17:53:22.87 Intake.select :open @17:53:25.05 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:53:25.07 SS.reconfigure SSinhale.with maxSpeed: 23 @17:53:25.11 SS.seek 25ml @17:53:27.88 AS.reconfigure ASslow2 @17:53:27.94 AS.seek 0.439ml @17:54:03.67 delay 1:00 @17:54:42.05 Stroke completed -- waiting for pressure to equalize ... @17:55:00.44 Intake.select :close @17:55:00.60 SSV.dial :exhaust @17:55:01.47 Exhaust.select :open @17:55:01.59 SS.reconfigure SSexhale @17:55:01.64 SS.seek 0ml @17:55:03.71 pushing eluate to holding coil @17:55:03.79 AS.seek 0.279ml @17:55:11.21 Exhaust.select :close @17:55:11.33 SSV.dial CBV @17:55:11.35 CBV.dial SSV @17:55:12.24 Allowing 130s for 25.0ml of remaining 200.0ml @17:55:12.26 Intake.select :open @17:55:14.44 Intake @15.9psia, vacuum from 9.4 to 13.4psi @17:55:14.46 SS.reconfigure SSinhale.with maxSpeed: 23 @17:55:14.51 SS.seek 25ml @17:56:31.28 Stroke completed -- waiting for pressure to equalize ... @17:56:49.74 Intake.select :close @17:56:49.91 SSV.dial :exhaust @17:56:50.78 Exhaust.select :open @17:56:51.36 SS.reconfigure SSexhale @17:56:51.51 SS.seek 0ml @17:57:01.11 Exhaust.select :close @17:57:01.23 SSV.dial CBV @17:57:01.26 CBV.dial SSV @17:57:02.13 Allowing 130s for 25.0ml of remaining 175.0ml @17:57:02.15 Intake.select :open @17:57:04.32 Intake @16.0psia, vacuum from 9.5 to 13.5psi @17:57:04.34 SS.reconfigure SSinhale.with maxSpeed: 23 @17:57:04.39 SS.seek 25ml @17:57:44.89 delay 30 seconds @17:58:14.92 ATV.dial :waste,via: :air3 @17:58:16.66 AS.reconfigure ASconfig @17:58:16.71 AS.seek :empty @17:58:21.17 Stroke completed -- waiting for pressure to equalize ... @17:58:33.09 Parking Bleach in LysateSource line and SPEcolumn @17:58:33.10 ATV.dial :air3 @17:58:34.00 AS.reconfigure ASslow1 @17:58:34.05 AS.seek 0.029ml @17:58:40.43 ATV.dial :ElutionWater @17:58:40.96 Intake.select :close @17:58:41.10 AS.seek 0.059ml @17:58:41.21 SSV.dial :exhaust @17:58:42.07 Exhaust.select :open @17:58:42.19 SS.reconfigure SSexhale @17:58:42.24 SS.seek 0ml @17:58:47.40 ATV.dial :air3 @17:58:48.03 AS.seek 0.069ml @17:58:50.35 delay 2 seconds @17:58:52.03 Exhaust.select :close @17:58:52.15 SSV.dial CBV @17:58:52.17 CBV.dial SSV @17:58:52.37 ATV.dial :bleach @17:58:52.83 AS.seek 0.769ml @17:58:53.05 Allowing 130s for 25.0ml of remaining 150.0ml @17:58:53.07 Intake.select :open @17:58:55.24 Intake @16.0psia, vacuum from 9.5 to 13.5psi @17:58:55.26 SS.reconfigure SSinhale.with maxSpeed: 23 @17:58:55.32 SS.seek 25ml @18:00:12.47 Stroke completed -- waiting for pressure to equalize ... @18:00:30.94 Intake.select :close @18:00:31.10 SSV.dial :exhaust @18:00:31.95 Exhaust.select :open @18:00:32.08 SS.reconfigure SSexhale @18:00:32.13 SS.seek 0ml @18:00:41.77 Exhaust.select :close @18:00:41.89 SSV.dial CBV @18:00:41.91 CBV.dial SSV @18:00:42.78 Allowing 130s for 25.0ml of remaining 125.0ml @18:00:42.80 Intake.select :open @18:00:44.98 Intake @16.0psia, vacuum from 9.5 to 13.5psi @18:00:45.00 SS.reconfigure SSinhale.with maxSpeed: 23 @18:00:45.04 SS.seek 25ml @18:01:13.98 delay 3 seconds @18:01:17.01 ATV.dial :air3 @18:01:17.56 delay 3 seconds @18:01:20.67 AS.seek 0.794ml @18:01:26.13 delay 3 seconds @18:01:29.16 ATV.dial :bleach @18:01:29.78 AS.seek 0.779ml @18:01:33.09 delay 3 seconds @18:01:36.12 ATV.dial :AIV @18:01:37.37 AS.reconfigure ASconfig @18:01:37.43 AS.seek 0.015ml @18:02:01.87 Stroke completed -- waiting for pressure to equalize ... @18:02:21.64 ATV.dial :waste @18:02:22.17 Intake.select :close @18:02:22.33 SSV.dial :exhaust @18:02:23.13 AS.seek :empty @18:02:23.20 Exhaust.select :open @18:02:23.32 SS.reconfigure SSexhale @18:02:23.37 SS.seek 0ml @18:02:24.37 ATV.dial :air3 @18:02:25.05 AS.reconfigure ASslow1 @18:02:25.11 AS.seek 0.009ml @18:02:27.42 delay 2 seconds @18:02:29.44 ATV.dial :bleach @18:02:29.89 AS.seek 0.579ml @18:02:32.95 Exhaust.select :close @18:02:33.07 SSV.dial CBV @18:02:33.09 CBV.dial SSV @18:02:33.95 Allowing 130s for 25.0ml of remaining 100.0ml @18:02:33.97 Intake.select :open @18:02:36.15 Intake @16.1psia, vacuum from 9.6 to 13.6psi @18:02:36.17 SS.reconfigure SSinhale.with maxSpeed: 23 @18:02:36.22 SS.seek 25ml @18:03:53.06 Stroke completed -- waiting for pressure to equalize ... @18:04:14.00 Intake.select :close @18:04:14.16 SSV.dial :exhaust @18:04:15.03 Exhaust.select :open @18:04:15.15 SS.reconfigure SSexhale @18:04:15.20 SS.seek 0ml @18:04:24.81 Exhaust.select :close @18:04:24.90 delay 3 seconds @18:04:24.95 SSV.dial CBV @18:04:24.97 CBV.dial SSV @18:04:25.86 Allowing 130s for 25.0ml of remaining 75.0ml @18:04:25.88 Intake.select :open @18:04:27.92 ATV.dial :air3 @18:04:28.06 Intake @16.1psia, vacuum from 9.6 to 13.6psi @18:04:28.08 SS.reconfigure SSinhale.with maxSpeed: 23 @18:04:28.13 SS.seek 25ml @18:04:28.48 delay 3 seconds @18:04:31.57 AS.seek 0.604ml @18:04:37.00 delay 3 seconds @18:04:40.03 ATV.dial :bleach @18:04:40.66 AS.seek 0.589ml @18:04:43.95 delay 3 seconds @18:04:46.98 ATV.dial :ASV @18:04:48.01 ASV.dial :wasteR @18:04:48.63 AS.seek 0ml @18:05:44.97 Stroke completed -- waiting for pressure to equalize ... @18:06:06.48 Intake.select :close @18:06:06.64 SSV.dial :exhaust @18:06:07.51 Exhaust.select :open @18:06:07.63 SS.reconfigure SSexhale @18:06:07.68 SS.seek 0ml @18:06:17.26 Exhaust.select :close @18:06:17.37 SSV.dial CBV @18:06:17.41 CBV.dial SSV @18:06:18.28 Allowing 130s for 25.0ml of remaining 50.0ml @18:06:18.30 Intake.select :open @18:06:20.48 Intake @16.1psia, vacuum from 9.6 to 13.6psi @18:06:20.50 SS.reconfigure SSinhale.with maxSpeed: 23 @18:06:20.55 SS.seek 25ml @18:06:47.37 delay 1:00 @18:07:27.69 Vacuum too high -- waiting up to 40s @18:07:40.75 Intake @16.0psia, vacuum from 9.5 to 13.5psi @18:07:40.77 SS.reconfigure SSinhale.with maxSpeed: 21 @18:07:40.81 SS.seek 25ml @18:07:47.40 AFV.select :carrierSolution @18:07:47.50 delay 2 seconds @18:07:49.53 AS.reconfigure ASconfig @18:07:49.58 AS.seek 1ml @18:07:51.69 Stroke completed -- waiting for pressure to equalize ... @18:08:01.95 Intake.select :close @18:08:02.11 SSV.dial :exhaust @18:08:02.97 Exhaust.select :open @18:08:03.11 SS.reconfigure SSexhale @18:08:03.16 SS.seek 0ml @18:08:12.83 Exhaust.select :close @18:08:12.95 SSV.dial CBV @18:08:12.97 CBV.dial SSV @18:08:13.84 Allowing 130s for 25.0ml of remaining 25.0ml @18:08:13.86 Intake.select :open @18:08:16.04 Intake @16.1psia, vacuum from 9.6 to 13.6psi @18:08:16.06 SS.reconfigure SSinhale.with maxSpeed: 21 @18:08:16.11 SS.seek 24.99ml @18:08:47.35 delay 1 second @18:08:48.38 AFV.select :holdingCoil @18:08:48.48 delay 1 second @18:08:49.51 ATV.dial ASV @18:08:49.52 ASV.dial :wasteR @18:08:49.54 AS.reconfigure ASslow1 @18:08:49.59 AS.seek 0ml @18:09:40.19 Stroke completed -- waiting for pressure to equalize ... @18:09:57.93 Intake.select :close @18:09:58.09 SSV.dial :exhaust @18:09:58.97 Exhaust.select :open @18:09:59.10 SS.reconfigure SSexhale @18:09:59.15 SS.seek 0ml @18:10:08.81 Exhaust.select :close @18:10:08.92 Sampled 400.0ml @18:10:08.95 Drying puck before fixing for 4:00 CBV.dial :waste2b @18:10:09.80 delay 1:00 @18:11:09.84 CSV.dial :air @18:11:10.35 CS.reconfigure CSfast @18:11:10.40 CS.seek 10ml @18:11:37.20 Pumping wcr puck from tube 2 up to 55psi... @18:11:37.22 CBV.dial SSV @18:11:38.64 CBV.dial between CSV and waste2b @18:11:39.73 CSV.dial between air and CTV @18:11:40.10 CS.seek 7.183ml @18:11:48.36 CTV.dial CSV,avoiding: :air @18:11:48.38 CSV.dial CTV @18:11:49.30 CS.reconfigure CSpressurize @18:11:49.35 CS.seek 0ml @18:12:05.03 CBV.dial :waste2b @18:12:05.38 CS.reconfigure CSconfig @18:12:05.44 CS.seek :empty @18:12:10.01 delay 1:00 @18:12:10.99 delay 20 seconds @18:12:31.02 ATV.dial :air3 @18:12:32.52 AS.seek 0.59ml @18:13:10.05 CTV.dial :air,avoiding: :intake @18:13:11.58 Fix #1 of 2 (with rnal) for 2:00 @18:13:11.59 CSV.dial CSR @18:13:12.14 Pulling 2ml of CSR.rnal @18:13:12.15 CSR.select :rnal @18:13:12.27 CS.seek 2ml @18:13:18.35 delay 5 seconds @18:13:23.38 CSR.select :air @18:13:23.50 CS.reconfigure CSfast @18:13:23.55 CS.seek 10ml @18:13:44.98 Pumping wcr puck from tube 2 up to 55psi... @18:13:45.00 CBV.dial SSV @18:13:46.41 CBV.dial between CSV and waste2b @18:13:47.50 CSV.dial between air and CTV @18:13:48.19 CS.seek 7.047ml @18:13:56.71 CTV.dial CSV,avoiding: :air @18:13:56.73 CSV.dial CTV @18:13:58.32 CS.reconfigure CSpressurize @18:13:58.37 CS.seek 0ml @18:14:15.50 delay 2:00 @18:14:31.48 delay 3 seconds @18:14:34.51 ATV.dial :ASV @18:14:35.92 ASV.dial :wasteR @18:14:36.01 AS.seek 0.09ml @18:16:15.53 CBV.dial :waste2b @18:16:15.90 CS.reconfigure CSconfig @18:16:15.96 CS.seek :empty @18:16:16.85 delay 30 seconds @18:16:18.51 delay 1:00 @18:16:46.88 ATV.dial :extSrc @18:16:47.74 delay 3 seconds @18:16:50.77 ATV.dial :ElutionWater @18:16:52.42 AS.seek 0.12ml @18:16:58.81 ATV.dial :air3 @18:16:59.53 AS.seek 0.13ml @18:17:01.87 delay 2 seconds @18:17:03.90 ATV.dial :bleach @18:17:04.38 AS.seek 0.23ml @18:17:18.54 CTV.dial :air,avoiding: :intake @18:17:20.07 Fix #2 of 2 (with rnal) for 2:00 @18:17:20.08 CSV.dial CSR @18:17:20.63 Pulling 2ml of CSR.rnal @18:17:20.64 CSR.select :rnal @18:17:20.76 CS.seek 2ml @18:17:24.82 delay 3 seconds @18:17:26.98 delay 5 seconds @18:17:27.85 ATV.dial :air3 @18:17:28.40 delay 3 seconds @18:17:31.49 AS.seek 0.255ml @18:17:32.01 CSR.select :air @18:17:32.13 CS.reconfigure CSfast @18:17:32.18 CS.seek 10ml @18:17:36.82 delay 3 seconds @18:17:39.85 ATV.dial :bleach @18:17:40.47 AS.seek 0.24ml @18:17:43.73 delay 3 seconds @18:17:46.76 ATV.dial :ASV @18:17:47.78 ASV.dial :SPEdelivery @18:17:48.17 AFV.select :carrierSolution @18:17:48.28 delay 2 seconds @18:17:50.31 AS.reconfigure ASconfig @18:17:50.36 AS.seek 0.5ml @18:17:53.82 Pumping wcr puck from tube 2 up to 55psi... @18:17:53.84 CBV.dial SSV @18:17:55.24 CBV.dial between CSV and waste2b @18:17:56.33 CSV.dial between air and CTV @18:17:57.02 CS.seek 6.987ml @18:18:05.63 delay 1 second @18:18:05.74 CTV.dial CSV,avoiding: :air @18:18:05.77 CSV.dial CTV @18:18:06.65 AFV.select :holdingCoil @18:18:06.81 AS.reconfigure ASslow1 @18:18:06.86 AS.seek 0.17ml @18:18:07.33 CS.reconfigure CSpressurize @18:18:07.38 CS.seek 0ml @18:18:23.19 delay 2:00 @18:19:13.50 SPE.hold 2:46:40,95C @18:19:13.55 delay 3:00 @18:20:23.23 CBV.dial :waste2b @18:20:23.62 CS.reconfigure CSconfig @18:20:23.67 CS.seek :empty @18:20:27.48 delay 1:00 @18:21:27.52 CTV.dial :air,avoiding: :intake @18:21:29.16 Storing wcr puck in Collection Clamp to tube 1 @18:21:29.36 SC.reconfigure SCtight @18:21:29.42 SC.seek 1 @18:21:31.55 SC.reconfigure SCloose @18:21:31.79 Elbow.reconfigure TightElbow @18:21:31.85 Elbow.seek 1 @18:21:33.82 Forearm.reconfigure TightForearm @18:21:33.87 Forearm.seek 1 + 700 counts @18:21:34.92 SE.reconfigure SEpick @18:21:35.00 SE.seek beamBreak @18:21:37.96 SE.configure SEconfig @18:21:38.67 Elbow.reconfigure LimpElbow @18:21:38.74 Forearm.reconfigure LooseForearm @18:21:38.79 Forearm.seek 1 @18:21:39.14 Elbow.hold @18:21:39.22 Elbow.reconfigure TightElbow @18:21:39.34 SE.seek 4.08 Pucks below Top Plate @18:21:39.84 Forearm.force -20 @18:21:39.89 Forearm.seek 1 + 320 counts @18:21:40.24 Forearm.reconfigure TightForearm @18:21:40.30 Forearm.seek 1 + 700 counts @18:21:40.62 SE.seek 5.08 Pucks below Top Plate @18:21:41.38 Forearm.reconfigure LooseForearm @18:21:41.43 Forearm.seek :retract @18:21:42.35 Elbow.seek CC @18:21:44.06 Elbow.reconfigure LooseElbow @18:21:44.18 Forearm.seek CC @18:21:45.14 Hand.close @18:21:45.94 CC.seek :open @18:21:50.47 Forearm.seek :retract @18:21:51.45 Elbow.reconfigure TightElbow @18:21:51.50 Elbow.seek 1 @18:21:53.40 Elbow.reconfigure LimpElbow @18:21:53.46 Forearm.seek 1 @18:21:54.50 Elbow.hold @18:21:54.58 Elbow.reconfigure TightElbow @18:21:54.63 Hand.open @18:21:55.58 Forearm.force -20 @18:21:55.63 Forearm.seek 1 + 345 counts @18:21:55.97 Forearm.reconfigure TightForearm @18:21:56.03 Forearm.seek 1 + 700 counts @18:21:56.35 SE.seek :down @18:21:57.50 Forearm.reconfigure LooseForearm @18:21:57.56 Forearm.seek :retract @18:21:58.84 SE.coast @18:21:58.88 SC.coast @18:21:58.93 Starting finalPurge @18:21:58.94 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:21:59.02 Hand.open @18:21:59.46 Elbow.seek FlushPuck::Garage @18:22:02.17 Elbow.reconfigure LooseElbow @18:22:02.28 Forearm.seek :garage @18:22:03.34 Hand.close @18:22:04.73 Forearm.seek :retract @18:22:05.81 Elbow.reconfigure TightElbow @18:22:05.86 Elbow.seek CC @18:22:08.82 Elbow.reconfigure LooseElbow @18:22:08.94 Forearm.reconfigure TightForearm @18:22:08.98 Forearm.seek CC @18:22:10.34 CC.seek :closed @18:22:13.61 AFV.select :carrierSolution @18:22:13.72 delay 2 seconds @18:22:14.60
Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.68 @18:22:14.70 Hand.open @18:22:15.51 Forearm.reconfigure LooseForearm @18:22:15.56 Forearm.seek :retract @18:22:15.74 AS.reconfigure ASconfig @18:22:15.80 AS.seek 1ml @18:22:16.58
Flushing the Collection Valve::Manifold @18:22:16.73 CSV.dial CSR @18:22:17.29 Pulling 5ml of CSR.flush @18:22:17.30 CSR.select :flush @18:22:17.43 CS.seek 5ml @18:22:31.89 delay 5 seconds @18:22:36.92 CSR.select :air @18:22:37.09 CS.reconfigure CSfast @18:22:37.14 CS.seek 7.501ml @18:22:44.86 CSV.dial :waste @18:22:46.41 CS.reconfigure CSconfig @18:22:46.45 CS.seek 0ml @18:23:03.79 delay 1 second @18:23:04.81 AFV.select :holdingCoil @18:23:04.91 delay 1 second @18:23:05.94 ASV.dial :wasteD @18:23:07.04 AS.seek 0ml @18:23:07.45
Flushing lower manifold's waste1 port @18:23:07.60 CSV.dial :flush @18:23:08.12 CS.reconfigure CSfast @18:23:08.17 CS.seek 5ml @18:23:22.45 delay 5 seconds @18:23:27.48 CSV.dial :air @18:23:28.08 CS.seek 7ml @18:23:34.43 CSV.dial CTV @18:23:34.45
CTV.dial CSV @18:23:36.12 CBV.dial :waste1 @18:23:37.01 CS.seek 0ml @18:23:56.61 Flushing lower manifold's waste2b port @18:23:56.75 CSV.dial :flush @18:23:57.62 CS.seek 5ml @18:24:04.82 Priming Eluate @18:24:04.83 AFV.select :carrierSolution @18:24:04.94 delay 2 seconds @18:24:06.98 AS.seek 0.5ml @18:24:11.98
delay 5 seconds @18:24:17.01 CSV.dial :air @18:24:17.63 CS.seek 7.001ml @18:24:23.93 CSV.dial CTV @18:24:23.96
CTV.dial CSV @18:24:24.45 CBV.dial :waste2b @18:24:25.34 CS.seek 0ml @18:24:35.99 delay 1 second @18:24:37.01 AFV.select :holdingCoil @18:24:37.11 ATV.dial ASV @18:24:37.12 ASV.dial :SPErecovery @18:24:37.74 Swish #1 of 2 @18:24:37.82 AS.reconfigure ASslow0 @18:24:37.87 AS.seek 0.6ml @18:24:44.58
Flushing lower manifold's waste1 port @18:24:45.12 CSV.dial CSR @18:24:45.73 Pulling 4ml of CSR.mfbkill @18:24:45.74 CSR.select :mfbkill @18:24:45.86 CS.reconfigure CSconfig @18:24:45.91 CS.seek 4ml @18:24:48.35 AS.seek 0.5ml @18:24:57.39
delay 5 seconds @18:24:58.79 Swish #2 of 2 @18:24:58.88 AS.seek 0.6ml @18:25:02.42
CSR.select :air @18:25:02.55 CS.reconfigure CSfast @18:25:02.60 CS.seek 10ml @18:25:09.31 AS.seek 0.5ml @18:25:19.31 CSV.dial CTV @18:25:19.34
CTV.dial CSV @18:25:19.71 delay 3 seconds @18:25:19.87
CBV.dial :waste1 @18:25:20.76 CS.seek 0ml @18:25:22.80 AS.reconfigure ASslow1 @18:25:22.85 AS.seek 0.684ml @18:25:47.89
Flushing lower manifold's waste2b port @18:25:47.90 CSV.dial CSR @18:25:48.60 Pulling 4ml of CSR.mfbkill @18:25:48.61 CSR.select :mfbkill @18:25:48.73 CS.reconfigure CSconfig @18:25:48.78 CS.seek 4ml @18:26:00.18 delay 3 seconds @18:26:00.23
delay 5 seconds @18:26:03.21 Eluate primed @18:26:03.22 ATV.dial :waste2 @18:26:03.85 AS.reconfigure ASnorm @18:26:03.90 AS.seek 0ml @18:26:05.26
CSR.select :air @18:26:05.39 CS.reconfigure CSfast @18:26:05.44 CS.seek 10ml @18:26:22.09 CSV.dial CTV @18:26:22.11
CTV.dial CSV @18:26:22.64 CBV.dial :waste2b @18:26:23.53 CS.seek 0ml @18:26:38.71 SPE.coast @18:26:38.79 ASV.dial :SPErecovery @18:26:38.82 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep1318h400ml.pcr @18:26:38.83 Building PCR Slug from ASV with MM2 mix, PP4 primer @18:26:38.85 Priming with carrier @18:26:38.86 ATV.dial ARV @18:26:39.82 ARV.dial :carrier @18:26:40.18 AS.seek 0.5ml @18:26:50.05
Killing upper manifold @18:26:50.06 CSV.dial CSR @18:26:50.65 Pulling 4ml of CSR.mfbkill @18:26:50.66 CSR.select :mfbkill @18:26:50.78 CS.reconfigure CSconfig @18:26:50.83 CS.seek 4ml @18:27:02.14 delay 5 seconds @18:27:05.74 AFV.select :carrierSolution @18:27:05.85 delay 2 seconds @18:27:07.17
CSR.select :air @18:27:07.34 CS.reconfigure CSfast @18:27:07.38 CS.seek 10ml @18:27:07.89 AS.reconfigure ASconfig @18:27:07.94 AS.seek 1ml @18:27:23.59 CSV.dial CBV @18:27:23.62
CBV.dial CSV @18:27:24.92 CTV.dial :air @18:27:26.43 CS.seek 0ml @18:27:36.95 delay 1 second @18:27:37.98 AFV.select :holdingCoil @18:27:38.08 delay 1 second @18:27:39.11 ATV.dial :waste @18:27:40.69 AS.reconfigure ASnorm @18:27:40.73 AS.seek :empty @18:27:52.49
delay 7 seconds @18:27:59.53 CS.seek 3ml @18:28:08.05 CSV.dial :waste @18:28:08.70 CS.seek 0ml @18:28:17.14 Flushing upper manifold @18:28:17.28 CSV.dial :flush @18:28:17.81 CS.seek 5ml @18:28:31.42 delay 5 seconds @18:28:31.51 AFV.select :carrierSolution @18:28:31.62 delay 2 seconds @18:28:33.65 AS.reconfigure ASconfig @18:28:33.70 AS.seek 0.5ml @18:28:36.45
CSV.dial :air @18:28:37.57 CS.seek 7ml @18:28:43.59 CSV.dial CBV @18:28:43.61
CBV.dial CSV @18:28:44.77 CTV.dial :air @18:28:44.80 CS.seek 0ml @18:29:02.79 delay 1 second @18:29:03.39
delay 7 seconds @18:29:03.82 AFV.select :holdingCoil @18:29:03.93 Building Slug @18:29:03.95 ATV.dial :ARV @18:29:05.63 delay 2 seconds @18:29:07.66 ARV.dial :air1 @18:29:08.07 delay 2 seconds @18:29:10.16 AS.reconfigure ASslow2 @18:29:10.22 AS.seek 0.506ml @18:29:10.42
CS.seek 3ml @18:29:16.59 delay 2 seconds @18:29:18.62 ARV.dial :MM2 @18:29:18.86
CSV.dial :waste @18:29:19.37 CS.seek 0ml @18:29:20.15 delay 2 seconds @18:29:22.18 Adding MM2 @18:29:22.27 AS.seek 0.524ml @18:29:28.09
CSV.dial :flush @18:29:28.70 CS.seek 3ml @18:29:37.17 delay 5 seconds @18:29:40.56 delay 25 seconds @18:29:42.19
CSV.dial :air @18:29:42.79 CS.seek 5ml @18:29:48.86 CSV.dial :air @18:29:48.88 CS.seek 0ml @18:30:02.39 delay 7 seconds @18:30:05.59 ARV.dial :PP4 @18:30:05.99 Adding PP4 @18:30:06.01 delay 2 seconds @18:30:08.10 AS.seek 0.53ml @18:30:09.42
CS.seek 3ml @18:30:14.43 delay 10 seconds @18:30:17.99
CSV.dial :waste @18:30:18.82 CS.seek 0ml @18:30:24.46 ARV.dial :air3 @18:30:24.87 adding ARV air @18:30:24.89 delay 2 seconds @18:30:26.97 AS.seek 0.536ml @18:30:27.30
Flushing lower manifold's waste1 port @18:30:27.44 CSV.dial :flush @18:30:27.97 CS.seek 5ml @18:30:33.42 delay 5 seconds @18:30:38.45 ARV.dial :carrier @18:30:39.74 delay 7 seconds @18:30:41.71
delay 5 seconds @18:30:46.74 CSV.dial :air @18:30:46.85 AS.seek 0.594ml @18:30:47.30
CS.seek 7ml @18:30:53.36 CSV.dial CTV @18:30:53.40
CTV.dial CSV @18:30:54.99 CBV.dial :waste1 @18:30:55.51 CS.seek 0ml @18:31:14.02 Flushing lower manifold's waste2b port @18:31:14.16 CSV.dial :flush @18:31:14.99 CS.seek 5ml @18:31:28.46 delay 5 seconds @18:31:33.49 CSV.dial :air @18:31:34.08 CS.seek 7ml @18:31:40.12 CSV.dial CTV @18:31:40.14
CTV.dial CSV @18:31:40.64 CBV.dial :waste2b @18:31:41.51 CS.seek 0ml @18:31:45.38 delay 5 seconds @18:31:50.41 ATV.dial :ASV @18:31:50.81 delay 2 seconds @18:31:52.91 AS.seek 0.6ml @18:31:59.15 delay 4 seconds @18:32:00.11
Garaging FlushPuck in Collection Clamp @18:32:00.74 Elbow.reconfigure TightElbow @18:32:00.79 Elbow.seek CC @18:32:01.06 Elbow.reconfigure LooseElbow @18:32:01.17 Forearm.seek CC @18:32:02.17 Hand.close @18:32:02.96 CC.seek :open @18:32:03.17 ATV.dial ARV @18:32:03.71 delay 2 seconds @18:32:05.74 ARV.dial :carrier @18:32:05.75 delay 2 seconds @18:32:07.35
Forearm.seek :retract @18:32:07.85 AS.seek 0.65ml @18:32:08.40
Elbow.reconfigure TightElbow @18:32:08.45 Elbow.seek :garage @18:32:11.61 Elbow.reconfigure LooseElbow @18:32:11.72 Forearm.reconfigure TightForearm @18:32:11.77 Forearm.seek :garage @18:32:12.93 Hand.open @18:32:13.77 Forearm.reconfigure LooseForearm @18:32:13.82 Forearm.seek :retract @18:32:14.88 Starting finalPurge @18:32:14.90 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:32:14.97 Hand.open @18:32:15.27 Elbow.reconfigure TightElbow @18:32:15.32 Elbow.seek FlushPuck::Garage @18:32:15.47 Elbow.reconfigure LooseElbow @18:32:15.58 Forearm.seek :garage @18:32:16.61 Hand.close @18:32:17.46 Forearm.seek :retract @18:32:18.51 Elbow.reconfigure TightElbow @18:32:18.56 Elbow.seek CC @18:32:21.45 Elbow.reconfigure LooseElbow @18:32:21.55 Forearm.reconfigure TightForearm @18:32:21.61 Forearm.seek CC @18:32:22.88 CC.seek :closed @18:32:27.15 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.30 @18:32:27.22 Hand.open @18:32:28.02 Forearm.reconfigure LooseForearm @18:32:28.07 Forearm.seek :retract @18:32:29.09 Flushing the Collection Valve::Manifold @18:32:29.24 CSV.dial CSR @18:32:29.80 Pulling 5ml of CSR.flush @18:32:29.81 CSR.select :flush @18:32:29.94 CS.reconfigure CSconfig @18:32:29.99 CS.seek 5ml @18:32:43.83 delay 5 seconds @18:32:48.86 CSR.select :air @18:32:49.04 CS.reconfigure CSfast @18:32:49.09 CS.seek 7.5ml @18:32:56.43 CSV.dial :waste @18:32:57.91 CS.reconfigure CSconfig @18:32:57.96 CS.seek 0ml @18:32:58.34 delay 4 seconds @18:33:02.37 ATV.dial PCR @18:33:02.98 ASV.dial :waste1 @18:33:03.77 ARV.dial :air1 @18:33:04.15 Positioning Slug by 0.324ml @18:33:04.82 AS.seek 0.326ml @18:33:18.18
Flushing lower manifold's waste1 port @18:33:18.32 CSV.dial :flush @18:33:18.83 CS.reconfigure CSfast @18:33:18.88 CS.seek 5ml @18:33:32.52 delay 5 seconds @18:33:37.55 CSV.dial :air @18:33:38.13 CS.seek 7ml @18:33:44.19 CSV.dial CTV @18:33:44.21
CTV.dial CSV @18:33:44.72 CBV.dial :waste1 @18:33:45.59 CS.seek 0ml @18:34:04.52 Flushing lower manifold's waste2b port @18:34:04.66 CSV.dial :flush @18:34:05.51 CS.seek 5ml @18:34:19.08 delay 5 seconds @18:34:24.11 CSV.dial :air @18:34:24.71 CS.seek 7ml @18:34:30.72 CSV.dial CTV @18:34:30.74
CTV.dial CSV @18:34:31.24 CBV.dial :waste2b @18:34:32.11 CS.seek 0ml @18:34:50.55 Disinfecting external Intake with kill @18:34:50.69 CSV.dial CSR @18:34:51.37 Pulling 8ml of CSR.kill @18:34:51.38 CSR.select :kill @18:34:51.50 CS.seek 8ml @18:35:12.68 delay 5 seconds @18:35:17.71 CSR.select :air @18:35:17.84 CS.seek 10ml @18:35:23.83 CSV.dial CBV @18:35:23.87
CBV.dial CSV @18:35:25.19 CTV.dial :intake @18:35:26.04 Intake.select :open @18:35:26.16 CS.seek 2ml @18:35:47.74 Intake.select :close @18:35:47.85 CTV.dial :air @18:35:48.72 CS.reconfigure CSconfig @18:35:48.76 CS.seek 0ml @18:35:54.96 CSV.dial CSR @18:35:56.17 Pulling 5ml of CSR.flush CSR.select :flush @18:35:56.30 CS.seek 5ml @18:36:10.08 delay 5 seconds @18:36:15.11 CSR.select :air @18:36:15.24 CS.seek 10ml @18:36:29.08 CSV.dial CBV @18:36:30.23 CS.seek 0ml @18:36:56.77 Garaging FlushPuck in Collection Clamp @18:36:56.86 Elbow.reconfigure TightElbow @18:36:56.91 Elbow.seek CC @18:36:57.16 Elbow.reconfigure LooseElbow @18:36:57.27 Forearm.seek CC @18:36:58.23 Hand.close @18:36:58.96 CC.seek :open @18:37:03.38 Forearm.seek :retract @18:37:04.37 Elbow.reconfigure TightElbow @18:37:04.42 Elbow.seek :garage @18:37:07.48 Elbow.reconfigure LooseElbow @18:37:07.59 Forearm.reconfigure TightForearm @18:37:07.64 Forearm.seek :garage @18:37:08.81 Hand.open @18:37:09.65 Forearm.reconfigure LooseForearm @18:37:09.70 Forearm.seek :retract @18:37:10.78 Completed [mbaPhase] begun 16:29:35 @18:37:10.80 Extending 18tun1 until completion of MFBPCR @18:38:31.13 delay 5 seconds @18:38:36.16 ATV.dial ARV @18:38:37.02 ASV.dial :PCRlock @18:38:37.38 delay 5 seconds @18:38:42.41 Running Tt1 Profile60C300 thermocycling @18:38:42.42 PCR.program Profile60C300 @18:38:42.56 Profile60C300 should complete in < 1:45:21 @18:38:42.57 PCR.run 2:16:41.25 @20:04:16.77 PCR.off @20:04:18.72 PCR for Tt1 completed -- closed /var/log/esp/Tt118sep1318h400ml.pcr @20:04:18.80 Cleaning PCR @20:04:18.81 AFV.select :carrierSolution @20:04:18.92 delay 2 seconds @20:04:20.96 AS.reconfigure ASconfig @20:04:21.00 AS.seek 0.5ml @20:04:31.29 delay 1 second @20:04:32.31 AFV.select :holdingCoil @20:04:32.41 ASV.dial :waste1 @20:04:32.80 ATV.dial :air3 @20:04:34.06 AS.reconfigure ASslow1 @20:04:34.12 AS.seek 0.51ml @20:04:36.40 delay 2 seconds @20:04:38.43 ATV.dial :bleach @20:04:39.06 AS.seek 0.81ml @20:05:39.69 delay 3 seconds @20:05:42.72 ATV.dial :air3 @20:05:43.26 delay 3 seconds @20:05:46.35 AS.seek 0.835ml @20:05:51.63 delay 3 seconds @20:05:54.66 ATV.dial :bleach @20:05:55.26 AS.seek 0.82ml @20:05:58.52 delay 3 seconds @20:06:01.55 ARV.dial :waste @20:06:02.16 delay 3 seconds @20:06:05.19 ATV.dial :ARV @20:06:06.06 AS.reconfigure ASconfig @20:06:06.11 AS.seek 0.47ml @20:06:26.54 AFV.select :carrierSolution @20:06:26.65 delay 2 seconds @20:06:28.69 AS.seek 1ml @20:06:59.47 delay 1 second @20:07:00.49 AFV.select :holdingCoil @20:07:00.59 delay 1 second @20:07:01.62 AS.reconfigure ASfast @20:07:01.68 AS.seek 0ml @20:07:29.00 AFV.select :carrierSolution @20:07:29.11 delay 2 seconds @20:07:31.14 AS.reconfigure ASconfig @20:07:31.19 AS.seek 1ml @20:08:28.96 delay 1 second @20:08:29.98 AFV.select :holdingCoil @20:08:30.08 delay 1 second @20:08:31.11 AS.reconfigure ASfast @20:08:31.16 AS.seek 0ml @20:08:58.50 AFV.select :carrierSolution @20:08:58.61 delay 2 seconds @20:09:00.64 AS.reconfigure ASconfig @20:09:00.69 AS.seek 1ml @20:09:58.44 delay 1 second @20:09:59.46 AFV.select :holdingCoil @20:09:59.56 delay 1 second @20:10:00.59 AS.reconfigure ASfast @20:10:00.64 AS.seek 0ml @20:10:27.99 ARV.dial between carrier and air1 @20:10:28.49 ATV.dial :air3 @20:10:29.76 AS.reconfigure ASslow1 @20:10:29.82 AS.seek 0.01ml @20:10:32.13 delay 2 seconds @20:10:34.16 ATV.dial :bleach @20:10:34.78 AS.seek 0.81ml @20:13:16.05 delay 3 seconds @20:13:19.07 ATV.dial :air3 @20:13:19.61 delay 3 seconds @20:13:22.70 AS.seek 0.835ml @20:13:28.06 delay 3 seconds @20:13:31.09 ATV.dial :bleach @20:13:31.70 AS.seek 0.82ml @20:13:34.97 delay 3 seconds @20:13:38.00 ATV.dial PCR @20:13:38.40 AS.reconfigure ASconfig @20:13:38.45 AS.seek :empty @20:14:25.90 PCR.program :bleachProfile @20:14:25.96 PCR program should complete in < 2:45 @20:14:25.97 PCR.run 10:00 @20:17:30.82 PCR.off @20:17:30.85 AFV.select :carrierSolution @20:17:30.96 delay 2 seconds @20:17:33.00 AS.seek 1ml @20:18:30.83 delay 1 second @20:18:32.34 AFV.select :holdingCoil @20:18:32.51 delay 1 second @20:18:33.55 AS.reconfigure ASfast @20:18:33.59 AS.seek 0ml @20:19:00.94 delay 3 seconds @20:19:03.97 AFV.select :carrierSolution @20:19:04.07 delay 2 seconds @20:19:06.10 AS.reconfigure ASconfig @20:19:06.16 AS.seek 1ml @20:20:03.91 delay 1 second @20:20:04.94 AFV.select :holdingCoil @20:20:05.04 delay 1 second @20:20:06.07 AS.reconfigure ASfast @20:20:06.12 AS.seek 0ml @20:20:33.44 delay 3 seconds @20:20:36.47 AFV.select :carrierSolution @20:20:36.57 delay 2 seconds @20:20:38.60 AS.reconfigure ASconfig @20:20:38.66 AS.seek 1ml @20:21:36.41 delay 1 second @20:21:37.44 AFV.select :holdingCoil @20:21:37.54 delay 1 second @20:21:38.57 AS.reconfigure ASfast @20:21:38.62 AS.seek 0ml @20:22:05.96 delay 3 seconds @20:22:08.99 AFV.select :carrierSolution @20:22:09.09 delay 2 seconds @20:22:11.12 AS.reconfigure ASconfig @20:22:11.18 AS.seek 1ml @20:23:08.96 delay 1 second @20:23:09.98 AFV.select :holdingCoil @20:23:10.08 delay 1 second @20:23:11.12 AS.reconfigure ASfast @20:23:11.16 AS.seek 0ml @20:23:38.50 delay 3 seconds @20:23:41.53 AFV.select :carrierSolution @20:23:41.63 delay 2 seconds @20:23:43.66 AS.reconfigure ASconfig @20:23:43.72 AS.seek 1ml @20:24:41.49 delay 1 second @20:24:42.51 AFV.select :holdingCoil @20:24:42.61 delay 1 second @20:24:43.64 AS.reconfigure ASnorm @20:24:43.69 AS.seek 0ml @20:25:34.41 Setting up PCR for Rp -- writing /var/log/esp/Rp18sep1320h400ml.pcr @20:25:34.43 Building PCR Slug from ASV with MM2 mix, PP3 primer @20:25:34.45 Priming with carrier @20:25:34.46 ATV.dial ARV @20:25:35.05 ARV.dial :carrier @20:25:35.40 AS.seek 0.5ml @20:26:01.04 AFV.select :carrierSolution @20:26:01.15 delay 2 seconds @20:26:03.19 AS.reconfigure ASconfig @20:26:03.23 AS.seek 1ml @20:26:32.24 delay 1 second @20:26:33.26 AFV.select :holdingCoil @20:26:33.36 delay 1 second @20:26:34.39 ATV.dial :waste @20:26:36.00 AS.reconfigure ASnorm @20:26:36.05 AS.seek :empty @20:27:26.79 AFV.select :carrierSolution @20:27:26.90 delay 2 seconds @20:27:28.94 AS.reconfigure ASconfig @20:27:29.03 AS.seek 0.5ml @20:27:58.16 delay 1 second @20:27:59.21 AFV.select :holdingCoil @20:27:59.31 Building Slug @20:27:59.33 ATV.dial :ARV @20:28:00.91 delay 2 seconds @20:28:02.94 ARV.dial :air1 @20:28:03.33 delay 2 seconds @20:28:05.42 AS.reconfigure ASslow2 @20:28:05.47 AS.seek 0.506ml @20:28:11.82 delay 2 seconds @20:28:13.85 ARV.dial :MM2 @20:28:15.36 delay 2 seconds @20:28:17.39 Adding MM2 @20:28:17.47 AS.seek 0.524ml @20:28:35.82 delay 25 seconds @20:29:00.85 ARV.dial :PP3 @20:29:01.25 Adding PP3 @20:29:01.27 delay 2 seconds @20:29:03.35 AS.seek 0.53ml @20:29:09.71 delay 10 seconds @20:29:19.74 ARV.dial :air2 @20:29:20.14 adding ARV air @20:29:20.16 delay 2 seconds @20:29:22.24 AS.seek 0.536ml @20:29:28.61 delay 5 seconds @20:29:33.64 ARV.dial :carrier @20:29:34.94 delay 7 seconds @20:29:42.03 AS.seek 0.594ml @20:30:40.58 delay 5 seconds @20:30:45.61 ATV.dial :ASV @20:30:46.00 delay 2 seconds @20:30:48.09 AS.seek 0.6ml @20:30:54.44 delay 4 seconds @20:30:58.47 ATV.dial ARV @20:30:59.00 delay 2 seconds @20:31:01.03 ARV.dial :carrier @20:31:01.04 delay 2 seconds @20:31:03.12 AS.seek 0.65ml @20:31:53.63 delay 4 seconds @20:31:57.66 ATV.dial PCR @20:31:58.35 ASV.dial :waste1 @20:31:58.37 ARV.dial :air1 @20:31:58.79 Positioning Slug by 0.324ml @20:31:59.44 AS.seek 0.326ml @20:37:25.52 delay 5 seconds @20:37:30.55 ATV.dial ARV @20:37:31.30 ASV.dial :PCRlock @20:37:31.69 delay 5 seconds @20:37:36.72 Running Rp Profile60C300 thermocycling @20:37:36.73 PCR.program Profile60C300 @20:37:36.87 Profile60C300 should complete in < 1:45:21 @20:37:36.88 PCR.run 2:16:41.25 @22:02:36.07 PCR.off @22:02:37.96 PCR for Rp completed -- closed /var/log/esp/Rp18sep1320h400ml.pcr @22:02:38.03 Cleaning PCR @22:02:38.04 AFV.select :carrierSolution @22:02:38.15 delay 2 seconds @22:02:40.18 AS.reconfigure ASconfig @22:02:40.24 AS.seek 0.5ml @22:02:50.55 delay 1 second @22:02:51.57 AFV.select :holdingCoil @22:02:51.67 ASV.dial :waste1 @22:02:52.05 ATV.dial :air3 @22:02:53.32 AS.reconfigure ASslow1 @22:02:53.37 AS.seek 0.51ml @22:02:55.67 delay 2 seconds @22:02:57.69 ATV.dial :bleach @22:02:58.32 AS.seek 0.81ml @22:03:59.00 delay 3 seconds @22:04:02.03 ATV.dial :air3 @22:04:02.56 delay 3 seconds @22:04:05.65 AS.seek 0.835ml @22:04:10.98 delay 3 seconds @22:04:14.01 ATV.dial :bleach @22:04:14.73 AS.seek 0.82ml @22:04:18.03 delay 3 seconds @22:04:21.06 ARV.dial :waste @22:04:21.67 delay 3 seconds @22:04:24.70 ATV.dial :ARV @22:04:25.57 AS.reconfigure ASconfig @22:04:25.62 AS.seek 0.47ml @22:04:46.03 AFV.select :carrierSolution @22:04:46.14 delay 2 seconds @22:04:48.18 AS.seek 1ml @22:05:18.92 delay 1 second @22:05:19.94 AFV.select :holdingCoil @22:05:20.05 delay 1 second @22:05:21.07 AS.reconfigure ASfast @22:05:21.12 AS.seek 0ml @22:05:48.45 AFV.select :carrierSolution @22:05:48.56 delay 2 seconds @22:05:50.59 AS.reconfigure ASconfig @22:05:50.64 AS.seek 1ml @22:06:48.41 delay 1 second @22:06:49.43 AFV.select :holdingCoil @22:06:49.53 delay 1 second @22:06:50.56 AS.reconfigure ASfast @22:06:50.61 AS.seek 0ml @22:07:17.95 AFV.select :carrierSolution @22:07:18.06 delay 2 seconds @22:07:20.09 AS.reconfigure ASconfig @22:07:20.14 AS.seek 1ml @22:08:17.89 delay 1 second @22:08:18.91 AFV.select :holdingCoil @22:08:19.01 delay 1 second @22:08:20.04 AS.reconfigure ASfast @22:08:20.09 AS.seek 0ml @22:08:47.44 ARV.dial between carrier and air1 @22:08:48.05 ATV.dial :air3 @22:08:49.32 AS.reconfigure ASslow1 @22:08:49.37 AS.seek 0.01ml @22:08:51.73 delay 2 seconds @22:08:53.75 ATV.dial :bleach @22:08:54.38 AS.seek 0.81ml @22:11:35.64 delay 3 seconds @22:11:38.67 ATV.dial :air3 @22:11:39.20 delay 3 seconds @22:11:42.29 AS.seek 0.835ml @22:11:47.67 delay 3 seconds @22:11:50.70 ATV.dial :bleach @22:11:51.32 AS.seek 0.82ml @22:11:54.59 delay 3 seconds @22:11:57.62 ATV.dial PCR @22:11:58.00 AS.reconfigure ASconfig @22:11:58.05 AS.seek :empty @22:12:45.52 PCR.program :bleachProfile @22:12:45.58 PCR program should complete in < 2:45 @22:12:45.59 PCR.run 10:00 @22:15:50.44 PCR.off @22:15:50.46 AFV.select :carrierSolution @22:15:50.57 delay 2 seconds @22:15:52.60 AS.seek 1ml @22:16:50.41 delay 1 second @22:16:51.43 AFV.select :holdingCoil @22:16:51.53 delay 1 second @22:16:53.11 AS.reconfigure ASfast @22:16:53.17 AS.seek 0ml @22:17:20.51 delay 3 seconds @22:17:23.53 AFV.select :carrierSolution @22:17:23.63 delay 2 seconds @22:17:25.66 AS.reconfigure ASconfig @22:17:25.71 AS.seek 1ml @22:18:23.48 delay 1 second @22:18:24.51 AFV.select :holdingCoil @22:18:24.61 delay 1 second @22:18:25.65 AS.reconfigure ASfast @22:18:25.69 AS.seek 0ml @22:18:53.05 delay 3 seconds @22:18:56.08 AFV.select :carrierSolution @22:18:56.18 delay 2 seconds @22:18:58.21 AS.reconfigure ASconfig @22:18:58.28 AS.seek 1ml @22:19:56.03 delay 1 second @22:19:57.05 AFV.select :holdingCoil @22:19:57.15 delay 1 second @22:19:58.17 AS.reconfigure ASfast @22:19:58.22 AS.seek 0ml @22:20:25.56 delay 3 seconds @22:20:28.59 AFV.select :carrierSolution @22:20:28.69 delay 2 seconds @22:20:30.72 AS.reconfigure ASconfig @22:20:30.78 AS.seek 1ml @22:21:28.52 delay 1 second @22:21:29.54 AFV.select :holdingCoil @22:21:29.64 delay 1 second @22:21:30.67 AS.reconfigure ASfast @22:21:30.72 AS.seek 0ml @22:21:58.06 delay 3 seconds @22:22:01.09 AFV.select :carrierSolution @22:22:01.19 delay 2 seconds @22:22:03.22 AS.reconfigure ASconfig @22:22:03.28 AS.seek 1ml @22:23:01.08 delay 1 second @22:23:02.10 AFV.select :holdingCoil @22:23:02.20 delay 1 second @22:23:03.23 AS.reconfigure ASnorm @22:23:03.29 AS.seek 0ml @22:23:54.00 Setting up PCR for Ss -- writing /var/log/esp/Ss18sep1322h400ml.pcr @22:23:54.02 Building PCR Slug from ASV with MM1 mix, PP2 primer @22:23:54.03 Priming with carrier @22:23:54.04 ATV.dial ARV @22:23:54.73 ARV.dial :carrier @22:23:55.10 AS.seek 0.5ml @22:24:20.66 AFV.select :carrierSolution @22:24:20.77 delay 2 seconds @22:24:22.81 AS.reconfigure ASconfig @22:24:22.85 AS.seek 1ml @22:24:51.86 delay 1 second @22:24:52.89 AFV.select :holdingCoil @22:24:52.99 delay 1 second @22:24:54.01 ATV.dial :waste @22:24:55.72 AS.reconfigure ASnorm @22:24:55.77 AS.seek :empty @22:25:46.50 AFV.select :carrierSolution @22:25:46.61 delay 2 seconds @22:25:48.65 AS.reconfigure ASconfig @22:25:48.70 AS.seek 0.5ml @22:26:17.81 delay 1 second @22:26:18.83 AFV.select :holdingCoil @22:26:18.93 Building Slug @22:26:18.94 ATV.dial :ARV @22:26:20.52 delay 2 seconds @22:26:22.55 ARV.dial :air1 @22:26:23.06 delay 2 seconds @22:26:25.14 AS.reconfigure ASslow2 @22:26:25.20 AS.seek 0.506ml @22:26:31.50 delay 2 seconds @22:26:33.53 ARV.dial :MM1 @22:26:34.14 delay 2 seconds @22:26:36.17 Adding MM1 @22:26:36.24 AS.seek 0.524ml @22:26:54.50 delay 25 seconds @22:27:19.53 ARV.dial :PP2 @22:27:19.92 Adding PP2 @22:27:19.94 delay 2 seconds @22:27:22.02 AS.seek 0.53ml @22:27:28.22 delay 10 seconds @22:27:38.25 ARV.dial :air2 @22:27:38.62 adding ARV air @22:27:38.64 delay 2 seconds @22:27:40.72 AS.seek 0.536ml @22:27:46.98 delay 5 seconds @22:27:52.01 ARV.dial :carrier @22:27:53.30 delay 7 seconds @22:28:00.39 AS.seek 0.594ml @22:28:58.91 delay 5 seconds @22:29:03.94 ATV.dial :ASV @22:29:04.33 delay 2 seconds @22:29:06.42 AS.seek 0.6ml @22:29:12.91 delay 4 seconds @22:29:16.94 ATV.dial ARV @22:29:17.48 delay 2 seconds @22:29:19.51 ARV.dial :carrier @22:29:19.52 delay 2 seconds @22:29:21.61 AS.seek 0.65ml @22:30:12.31 delay 4 seconds @22:30:16.34 ATV.dial PCR @22:30:17.03 ASV.dial :waste1 @22:30:17.05 ARV.dial :air1 @22:30:17.44 Positioning Slug by 0.324ml @22:30:18.09 AS.seek 0.326ml @22:35:44.11 delay 5 seconds @22:35:49.14 ATV.dial ARV @22:35:49.98 ASV.dial :PCRlock @22:35:50.37 delay 5 seconds @22:35:55.40 Running Ss Profile60C350 thermocycling @22:35:55.42 PCR.program Profile60C350 @22:35:55.56 Profile60C350 should complete in < 1:48:05 @22:35:55.57 PCR.run 2:20:06.25 @00:01:35.40CEST14-Sep-18 PCR.off @00:01:37.60 PCR for Ss completed -- closed /var/log/esp/Ss18sep1322h400ml.pcr @00:01:37.67 Cleaning PCR @00:01:37.69 AFV.select :carrierSolution @00:01:37.79 delay 2 seconds @00:01:39.82 AS.reconfigure ASconfig @00:01:39.87 AS.seek 0.5ml @00:01:50.18 delay 1 second @00:01:51.20 AFV.select :holdingCoil @00:01:51.30 ASV.dial :waste1 @00:01:51.68 ATV.dial :air3 @00:01:52.94 AS.reconfigure ASslow1 @00:01:52.99 AS.seek 0.51ml @00:01:55.29 delay 2 seconds @00:01:57.31 ATV.dial :bleach @00:01:57.93 AS.seek 0.81ml @00:02:58.51 delay 3 seconds @00:03:01.54 ATV.dial :air3 @00:03:02.09 delay 3 seconds @00:03:05.18 AS.seek 0.835ml @00:03:10.39 delay 3 seconds @00:03:13.42 ATV.dial :bleach @00:03:14.04 AS.seek 0.82ml @00:03:17.34 delay 3 seconds @00:03:20.37 ARV.dial :waste @00:03:20.98 delay 3 seconds @00:03:24.01 ATV.dial :ARV @00:03:24.88 AS.reconfigure ASconfig @00:03:24.93 AS.seek 0.47ml @00:03:45.36 AFV.select :carrierSolution @00:03:45.47 delay 2 seconds @00:03:47.51 AS.seek 1ml @00:04:18.28 delay 1 second @00:04:19.30 AFV.select :holdingCoil @00:04:19.40 delay 1 second @00:04:20.43 AS.reconfigure ASfast @00:04:20.48 AS.seek 0ml @00:04:47.82 AFV.select :carrierSolution @00:04:47.93 delay 2 seconds @00:04:49.96 AS.reconfigure ASconfig @00:04:50.01 AS.seek 1ml @00:05:47.78 delay 1 second @00:05:48.81 AFV.select :holdingCoil @00:05:48.91 delay 1 second @00:05:49.94 AS.reconfigure ASfast @00:05:49.99 AS.seek 0ml @00:06:17.33 AFV.select :carrierSolution @00:06:17.44 delay 2 seconds @00:06:19.47 AS.reconfigure ASconfig @00:06:19.52 AS.seek 1ml @00:07:17.31 delay 1 second @00:07:18.33 AFV.select :holdingCoil @00:07:18.43 delay 1 second @00:07:19.46 AS.reconfigure ASfast @00:07:19.51 AS.seek 0ml @00:07:46.87 ARV.dial between carrier and air1 @00:07:47.48 ATV.dial :air3 @00:07:48.75 AS.reconfigure ASslow1 @00:07:48.80 AS.seek 0.01ml @00:07:51.18 delay 2 seconds @00:07:53.21 ATV.dial :bleach @00:07:53.83 AS.seek 0.81ml @00:10:35.06 delay 3 seconds @00:10:38.09 ATV.dial :air3 @00:10:38.64 delay 3 seconds @00:10:41.73 AS.seek 0.835ml @00:10:47.07 delay 3 seconds @00:10:50.10 ATV.dial :bleach @00:10:50.72 AS.seek 0.82ml @00:10:54.15 delay 3 seconds @00:10:57.17 ATV.dial PCR @00:10:57.56 AS.reconfigure ASconfig @00:10:57.61 AS.seek :empty @00:11:45.09 PCR.program :bleachProfile @00:11:45.14 PCR program should complete in < 2:45 @00:11:45.15 PCR.run 10:00 @00:14:50.00 PCR.off @00:14:50.03 AFV.select :carrierSolution @00:14:50.14 delay 2 seconds @00:14:52.18 AS.seek 1ml @00:15:50.54 delay 1 second @00:15:51.56 AFV.select :holdingCoil @00:15:51.66 delay 1 second @00:15:52.70 AS.reconfigure ASfast @00:15:52.74 AS.seek 0ml @00:16:20.09 delay 3 seconds @00:16:23.12 AFV.select :carrierSolution @00:16:23.22 delay 2 seconds @00:16:25.25 AS.reconfigure ASconfig @00:16:25.30 AS.seek 1ml @00:17:23.06 delay 1 second @00:17:24.09 AFV.select :holdingCoil @00:17:24.19 delay 1 second @00:17:25.23 AS.reconfigure ASfast @00:17:25.27 AS.seek 0ml @00:17:52.61 delay 3 seconds @00:17:55.64 AFV.select :carrierSolution @00:17:55.74 delay 2 seconds @00:17:57.77 AS.reconfigure ASconfig @00:17:57.83 AS.seek 1ml @00:18:55.61 delay 1 second @00:18:56.63 AFV.select :holdingCoil @00:18:56.73 delay 1 second @00:18:57.76 AS.reconfigure ASfast @00:18:57.81 AS.seek 0ml @00:19:25.17 delay 3 seconds @00:19:28.20 AFV.select :carrierSolution @00:19:28.30 delay 2 seconds @00:19:30.33 AS.reconfigure ASconfig @00:19:30.39 AS.seek 1ml @00:20:28.23 delay 1 second @00:20:29.26 AFV.select :holdingCoil @00:20:29.36 delay 1 second @00:20:30.39 AS.reconfigure ASfast @00:20:30.44 AS.seek 0ml @00:20:57.81 delay 3 seconds @00:21:00.84 AFV.select :carrierSolution @00:21:00.94 delay 2 seconds @00:21:02.97 AS.reconfigure ASconfig @00:21:03.03 AS.seek 1ml @00:22:00.78 delay 1 second @00:22:01.80 AFV.select :holdingCoil @00:22:01.90 delay 1 second @00:22:02.93 AS.reconfigure ASnorm @00:22:02.98 AS.seek 0ml @00:22:53.75 Setting up PCR for Bb -- writing /var/log/esp/Bb18sep1400h400ml.pcr @00:22:53.77 Building PCR Slug from ASV with MM3 mix, PP5 primer @00:22:53.79 Priming with carrier @00:22:53.80 ATV.dial ARV @00:22:54.39 ARV.dial :carrier @00:22:54.74 AS.seek 0.5ml @00:23:20.31 AFV.select :carrierSolution @00:23:20.42 delay 2 seconds @00:23:22.46 AS.reconfigure ASconfig @00:23:22.50 AS.seek 1ml @00:23:51.54 delay 1 second @00:23:52.57 AFV.select :holdingCoil @00:23:52.67 delay 1 second @00:23:53.70 ATV.dial :waste @00:23:55.32 AS.reconfigure ASnorm @00:23:55.41 AS.seek :empty @00:24:46.23 AFV.select :carrierSolution @00:24:46.34 delay 2 seconds @00:24:48.37 AS.reconfigure ASconfig @00:24:48.43 AS.seek 0.5ml @00:25:17.53 delay 1 second @00:25:18.55 AFV.select :holdingCoil @00:25:18.65 Building Slug @00:25:18.66 ATV.dial :ARV @00:25:20.25 delay 2 seconds @00:25:22.28 ARV.dial :air1 @00:25:22.69 delay 2 seconds @00:25:24.78 AS.reconfigure ASslow2 @00:25:24.83 AS.seek 0.506ml @00:25:31.17 delay 2 seconds @00:25:33.20 ARV.dial :MM3 @00:25:34.25 delay 2 seconds @00:25:36.28 Adding MM3 @00:25:36.37 AS.seek 0.524ml @00:25:54.67 delay 25 seconds @00:26:19.70 ARV.dial :PP5 @00:26:20.09 Adding PP5 @00:26:20.11 delay 2 seconds @00:26:22.19 AS.seek 0.53ml @00:26:28.61 delay 10 seconds @00:26:38.64 ARV.dial :air3 @00:26:39.03 adding ARV air @00:26:39.05 delay 2 seconds @00:26:41.13 AS.seek 0.536ml @00:26:47.46 delay 5 seconds @00:26:52.49 ARV.dial :carrier @00:26:53.78 delay 7 seconds @00:27:00.87 AS.seek 0.594ml @00:27:59.54 delay 5 seconds @00:28:04.57 ATV.dial :ASV @00:28:04.96 delay 2 seconds @00:28:07.05 AS.seek 0.6ml @00:28:13.29 delay 4 seconds @00:28:17.32 ATV.dial ARV @00:28:17.86 delay 2 seconds @00:28:19.89 ARV.dial :carrier @00:28:19.90 delay 2 seconds @00:28:21.99 AS.seek 0.65ml @00:29:12.46 delay 4 seconds @00:29:16.49 ATV.dial PCR @00:29:17.18 ASV.dial :waste1 @00:29:17.19 ARV.dial :air1 @00:29:18.14 Positioning Slug by 0.324ml @00:29:18.23 AS.seek 0.326ml @00:34:44.32 delay 5 seconds @00:34:49.35 ATV.dial ARV @00:34:50.11 ASV.dial :PCRlock @00:34:50.50 delay 5 seconds @00:34:55.53 Running Bb Profile60C300 thermocycling @00:34:55.54 PCR.program Profile60C300 @00:34:55.68 Profile60C300 should complete in < 1:45:21 @00:34:55.69 PCR.run 2:16:41.25 @01:59:50.32 PCR.off @01:59:52.40 PCR for Bb completed -- closed /var/log/esp/Bb18sep1400h400ml.pcr @01:59:52.48 Cleaning PCR @01:59:52.49 AFV.select :carrierSolution @01:59:52.60 delay 2 seconds @01:59:54.63 AS.reconfigure ASconfig @01:59:54.68 AS.seek 0.5ml @02:00:04.99 delay 1 second @02:00:06.01 AFV.select :holdingCoil @02:00:06.11 ASV.dial :waste1 @02:00:06.49 ATV.dial :air3 @02:00:07.78 AS.reconfigure ASslow1 @02:00:07.83 AS.seek 0.51ml @02:00:10.13 delay 2 seconds @02:00:12.15 ATV.dial :bleach @02:00:12.77 AS.seek 0.81ml @02:01:13.35 delay 3 seconds @02:01:16.38 ATV.dial :air3 @02:01:16.92 delay 3 seconds @02:01:20.01 AS.seek 0.835ml @02:01:25.32 delay 3 seconds @02:01:28.35 ATV.dial :bleach @02:01:28.97 AS.seek 0.82ml @02:01:32.27 delay 3 seconds @02:01:35.30 ARV.dial :waste @02:01:35.90 delay 3 seconds @02:01:38.93 ATV.dial :ARV @02:01:39.80 AS.reconfigure ASconfig @02:01:39.85 AS.seek 0.47ml @02:02:00.26 AFV.select :carrierSolution @02:02:00.37 delay 2 seconds @02:02:02.41 AS.seek 1ml @02:02:33.16 delay 1 second @02:02:34.18 AFV.select :holdingCoil @02:02:34.28 delay 1 second @02:02:35.31 AS.reconfigure ASfast @02:02:35.36 AS.seek 0ml @02:03:02.71 AFV.select :carrierSolution @02:03:02.82 delay 2 seconds @02:03:04.85 AS.reconfigure ASconfig @02:03:04.90 AS.seek 1ml @02:04:02.66 delay 1 second @02:04:03.68 AFV.select :holdingCoil @02:04:03.78 delay 1 second @02:04:04.81 AS.reconfigure ASfast @02:04:04.86 AS.seek 0ml @02:04:32.20 AFV.select :carrierSolution @02:04:32.31 delay 2 seconds @02:04:34.34 AS.reconfigure ASconfig @02:04:34.39 AS.seek 1ml @02:05:32.15 delay 1 second @02:05:33.17 AFV.select :holdingCoil @02:05:33.27 delay 1 second @02:05:34.30 AS.reconfigure ASfast @02:05:34.35 AS.seek 0ml @02:06:01.71 ARV.dial between carrier and air1 @02:06:02.32 ATV.dial :air3 @02:06:03.59 AS.reconfigure ASslow1 @02:06:03.64 AS.seek 0.01ml @02:06:05.95 delay 2 seconds @02:06:07.97 ATV.dial :bleach @02:06:08.59 AS.seek 0.81ml @02:08:49.73 delay 3 seconds @02:08:52.75 ATV.dial :air3 @02:08:53.29 delay 3 seconds @02:08:56.38 AS.seek 0.835ml @02:09:01.79 delay 3 seconds @02:09:04.82 ATV.dial :bleach @02:09:05.42 AS.seek 0.82ml @02:09:08.71 delay 3 seconds @02:09:11.74 ATV.dial PCR @02:09:12.13 AS.reconfigure ASconfig @02:09:12.19 AS.seek :empty @02:09:59.65 PCR.program :bleachProfile @02:09:59.71 PCR program should complete in < 2:45 @02:09:59.72 PCR.run 10:00 @02:13:04.57 PCR.off @02:13:04.60 AFV.select :carrierSolution @02:13:04.71 delay 2 seconds @02:13:06.75 AS.seek 1ml @02:14:04.57 delay 1 second @02:14:05.59 AFV.select :holdingCoil @02:14:05.69 delay 1 second @02:14:07.28 AS.reconfigure ASfast @02:14:07.34 AS.seek 0ml @02:14:34.70 delay 3 seconds @02:14:37.72 AFV.select :carrierSolution @02:14:37.82 delay 2 seconds @02:14:39.85 AS.reconfigure ASconfig @02:14:39.92 AS.seek 1ml @02:15:37.68 delay 1 second @02:15:38.71 AFV.select :holdingCoil @02:15:38.81 delay 1 second @02:15:39.84 AS.reconfigure ASfast @02:15:39.90 AS.seek 0ml @02:16:07.23 delay 3 seconds @02:16:10.25 AFV.select :carrierSolution @02:16:10.35 delay 2 seconds @02:16:12.38 AS.reconfigure ASconfig @02:16:12.43 AS.seek 1ml @02:17:10.29 delay 1 second @02:17:11.31 AFV.select :holdingCoil @02:17:11.41 delay 1 second @02:17:12.44 AS.reconfigure ASfast @02:17:12.49 AS.seek 0ml @02:17:39.85 delay 3 seconds @02:17:42.88 AFV.select :carrierSolution @02:17:42.98 delay 2 seconds @02:17:45.01 AS.reconfigure ASconfig @02:17:45.07 AS.seek 1ml @02:18:42.84 delay 1 second @02:18:43.86 AFV.select :holdingCoil @02:18:43.96 delay 1 second @02:18:44.99 AS.reconfigure ASfast @02:18:45.04 AS.seek 0ml @02:19:12.40 delay 3 seconds @02:19:15.43 AFV.select :carrierSolution @02:19:15.53 delay 2 seconds @02:19:17.56 AS.reconfigure ASconfig @02:19:17.65 AS.seek 1ml @02:20:15.43 delay 1 second @02:20:16.45 AFV.select :holdingCoil @02:20:16.55 delay 1 second @02:20:17.58 AS.reconfigure ASnorm @02:20:17.63 AS.seek 0ml @02:21:08.34 Setting up PCR for IPc -- writing /var/log/esp/IPc18sep1402h400ml.pcr @02:21:08.36 Building PCR Slug from ASV with MM1 mix, PP1 primer @02:21:08.38 Priming with carrier ATV.dial ARV @02:21:09.07 ARV.dial :carrier @02:21:09.43 AS.seek 0.5ml @02:21:34.98 AFV.select :carrierSolution @02:21:35.09 delay 2 seconds @02:21:37.12 AS.reconfigure ASconfig @02:21:37.17 AS.seek 1ml @02:22:06.20 delay 1 second @02:22:07.23 AFV.select :holdingCoil @02:22:07.33 delay 1 second @02:22:08.36 ATV.dial :waste @02:22:10.08 AS.reconfigure ASnorm @02:22:10.13 AS.seek :empty @02:23:00.90 AFV.select :carrierSolution @02:23:01.01 delay 2 seconds @02:23:03.04 AS.reconfigure ASconfig @02:23:03.09 AS.seek 0.5ml @02:23:32.18 delay 1 second @02:23:33.20 AFV.select :holdingCoil @02:23:33.30 Building Slug @02:23:33.31 ATV.dial :ARV @02:23:34.90 delay 2 seconds @02:23:36.93 ARV.dial :air1 @02:23:37.32 delay 2 seconds @02:23:39.41 AS.reconfigure ASslow2 @02:23:39.46 AS.seek 0.506ml @02:23:46.02 delay 2 seconds @02:23:48.04 ARV.dial :MM1 @02:23:48.65 delay 2 seconds @02:23:50.68 Adding MM1 @02:23:50.75 AS.seek 0.524ml @02:24:09.16 delay 25 seconds @02:24:34.19 ARV.dial :PP1 @02:24:34.59 Adding PP1 @02:24:34.61 delay 2 seconds @02:24:36.69 AS.seek 0.53ml @02:24:43.04 delay 10 seconds @02:24:53.08 ARV.dial :air1 @02:24:53.46 adding ARV air @02:24:53.48 delay 2 seconds @02:24:55.56 AS.seek 0.536ml @02:25:01.93 delay 5 seconds @02:25:06.96 ARV.dial :carrier @02:25:07.37 delay 7 seconds @02:25:14.46 AS.seek 0.594ml @02:26:13.02 delay 5 seconds @02:26:18.05 ATV.dial :ASV @02:26:18.45 delay 2 seconds @02:26:20.54 AS.seek 0.6ml @02:26:26.79 delay 4 seconds @02:26:30.82 ATV.dial ARV @02:26:31.35 delay 2 seconds @02:26:33.37 ARV.dial :carrier @02:26:33.38 delay 2 seconds @02:26:35.47 AS.seek 0.65ml @02:27:25.99 delay 4 seconds @02:27:30.02 ATV.dial PCR @02:27:30.62 ASV.dial :waste1 @02:27:30.64 ARV.dial :air1 @02:27:31.05 Positioning Slug by 0.324ml @02:27:31.14 AS.seek 0.326ml @02:32:57.60 delay 5 seconds @02:33:02.63 ATV.dial ARV @02:33:03.40 ASV.dial :PCRlock @02:33:03.79 delay 5 seconds @02:33:08.82 Running IPc ProfileIPc thermocycling @02:33:08.83 PCR.program ProfileIPc @02:33:08.98 ProfileIPc should complete in < 1:50:49 @02:33:08.99 PCR.run 2:23:31.25 @04:00:38.21 PCR.off @04:00:40.94 PCR for IPc completed -- closed /var/log/esp/IPc18sep1402h400ml.pcr @04:00:40.95 Full MFB Decontamination @04:00:40.97 Decon extraction column and decon AIV @04:00:40.99 SPE.hold 2:46:40,55C @04:00:41.04 ASV.dial :SPEdelivery @04:00:41.71 delay 20 seconds @04:01:01.75 AFV.select :carrierSolution @04:01:01.85 delay 2 seconds @04:01:03.88 AS.reconfigure ASconfig @04:01:03.93 AS.seek 0.5ml @04:01:14.26 delay 1 second @04:01:15.29 AFV.select :holdingCoil @04:01:15.41 ATV.dial :air1 @04:01:17.33 AS.reconfigure ASslow1 @04:01:17.38 AS.seek 0.52ml @04:01:21.69 ATV.dial :ASV @04:01:23.47 ASV.dial :SPErecovery @04:01:24.15 AS.seek 0.62ml @04:01:44.52 ATV.dial :waste @04:01:46.39 AS.reconfigure ASnorm @04:01:46.44 AS.seek :empty @04:02:18.07 AFV.select :holdingCoil @04:02:18.08 ATV.dial :waste @04:02:18.10 AS.reconfigure ASconfig @04:02:18.15 AS.seek 0ml @04:02:18.46 AFV.select :carrierSolution @04:02:18.57 delay 2 seconds @04:02:20.61 AS.seek 0.5ml @04:02:49.63 delay 1 second @04:02:50.65 AFV.select :holdingCoil @04:02:50.75 ATV.dial :air3 @04:02:51.64 AS.reconfigure ASslow1 @04:02:51.69 AS.seek 0.52ml @04:02:55.99 ATV.dial :air3 @04:02:56.08 AS.seek 0.53ml @04:02:58.30 delay 2 seconds @04:03:00.32 ATV.dial :bleach @04:03:00.81 AS.seek 0.71ml @04:03:37.38 delay 3 seconds @04:03:40.41 ATV.dial :air3 @04:03:40.97 delay 3 seconds @04:03:44.06 AS.seek 0.735ml @04:03:49.38 delay 3 seconds @04:03:52.41 ATV.dial :bleach @04:03:53.04 AS.seek 0.72ml @04:03:56.38 delay 3 seconds @04:03:59.41 ATV.dial ASV @04:04:00.49 ASV.dial :SPErecovery @04:04:00.58 AS.seek 0.365ml @04:05:12.26 ATV.dial :waste @04:05:14.00 AS.reconfigure ASnorm @04:05:14.05 AS.seek :empty @04:05:32.83 ATV.dial :air3 @04:05:33.51 AS.reconfigure ASslow1 @04:05:34.10 AS.seek 0.009ml @04:05:36.47 delay 2 seconds @04:05:38.50 ATV.dial :bleach @04:05:38.96 AS.seek 0.579ml @04:07:33.83 delay 3 seconds @04:07:36.86 ATV.dial :air3 @04:07:37.41 delay 3 seconds @04:07:40.51 AS.seek 0.604ml @04:07:45.83 delay 3 seconds @04:07:48.86 ATV.dial :bleach @04:07:49.48 AS.seek 0.589ml @04:07:52.79 delay 3 seconds @04:07:55.82 ATV.dial :ASV @04:07:56.85 ASV.dial :wasteR @04:07:57.34 AS.seek 0ml @04:09:56.11 delay 1:00 @04:10:56.14 ATV.dial ASV @04:10:56.15 AFV.select :carrierSolution @04:10:56.26 delay 2 seconds @04:10:58.29 AS.reconfigure ASconfig @04:10:58.34 AS.seek 1ml @04:11:56.08 delay 1 second @04:11:57.10 AFV.select :holdingCoil @04:11:57.20 delay 1 second @04:11:58.23 ASV.dial :wasteR @04:11:58.25 AS.reconfigure ASslow1 @04:11:58.29 AS.seek 0ml @04:15:19.71 delay 10 seconds @04:15:29.74 ATV.dial :air3 @04:15:31.24 AS.seek 0.01ml @04:15:33.55 delay 2 seconds @04:15:35.58 ATV.dial :bleach @04:15:36.20 AS.seek 0.58ml @04:17:31.10 delay 3 seconds @04:17:34.12 ATV.dial :air3 @04:17:34.68 delay 3 seconds @04:17:37.77 AS.seek 0.605ml @04:17:43.17 delay 3 seconds @04:17:46.20 ATV.dial :bleach @04:17:46.83 AS.seek 0.59ml @04:17:50.13 delay 3 seconds @04:17:53.16 ATV.dial ASV @04:17:54.18 ASV.dial :SPEdelivery @04:17:54.56 AS.seek 0ml @04:19:53.61 delay 1:00 @04:20:53.64 AFV.select :carrierSolution @04:20:53.74 delay 2 seconds @04:20:55.78 AS.reconfigure ASconfig @04:20:55.82 AS.seek 1ml @04:21:53.57 delay 1 second @04:21:54.59 AFV.select :holdingCoil @04:21:54.69 delay 1 second @04:21:55.72 ATV.dial ASV @04:21:55.73 ASV.dial :SPEdelivery @04:21:55.75 AS.reconfigure ASslow1 @04:21:55.80 AS.seek 0ml @04:25:17.30 delay 10 seconds @04:25:27.33 ATV.dial :air2 @04:25:28.14 AS.reconfigure ASslow0 @04:25:28.19 AS.seek 0.8ml @04:26:49.01 delay 3 seconds @04:26:52.04 ATV.dial :ASV @04:26:53.11 ASV.dial :SPEdelivery @04:26:53.13 AS.reconfigure ASslow1 @04:26:53.18 AS.seek 0.03ml @04:29:28.26 delay 20 seconds @04:29:48.29 ATV.dial :waste,via: :air3 @04:29:49.99 AS.reconfigure ASconfig @04:29:50.05 AS.seek :empty @04:29:52.11 SPE.coast @04:29:52.19 Removing Bleach from Mixing Coil @04:29:52.21 AFV.select :carrierSolution @04:29:52.31 delay 2 seconds @04:29:54.35 AS.seek 0.5ml @04:30:23.44 delay 1 second @04:30:24.46 AFV.select :holdingCoil @04:30:24.56 ATV.dial :ASV @04:30:26.22 ASV.dial :SPErecovery @04:30:26.86 AS.reconfigure ASslow1 @04:30:26.91 AS.seek 0.73ml @04:31:13.44 ASV.dial :wasteD @04:31:14.11 AS.reconfigure ASnorm @04:31:14.16 AS.seek 0ml @04:31:51.29 AFV.select :carrierSolution @04:31:51.40 delay 2 seconds @04:31:53.43 AS.reconfigure ASconfig @04:31:53.48 AS.seek 0.5ml @04:32:22.54 delay 1 second @04:32:23.56 AFV.select :holdingCoil @04:32:23.66 ATV.dial ASV @04:32:23.67 ASV.dial :SPErecovery @04:32:24.33 AS.reconfigure ASslow0 @04:32:24.38 AS.seek 0.3ml @04:32:44.85 ATV.dial :air2 @04:32:46.44 AS.reconfigure ASslow1 @04:32:46.52 AS.seek 0.32ml @04:32:50.86 ATV.dial ASV @04:32:51.89 AS.reconfigure ASslow0 @04:32:51.95 AS.seek 0.57ml @04:33:17.43 ASV.dial :wasteD @04:33:18.03 AS.reconfigure ASnorm @04:33:18.08 AS.seek 0ml @04:33:47.15 AFV.select :carrierSolution @04:33:47.26 delay 2 seconds @04:33:49.29 AS.reconfigure ASconfig @04:33:49.34 AS.seek 0.5ml @04:34:18.40 delay 1 second @04:34:19.43 AFV.select :holdingCoil @04:34:19.53 ATV.dial ASV @04:34:19.54 ASV.dial :SPErecovery @04:34:20.20 AS.reconfigure ASslow0 @04:34:20.26 AS.seek 0.3ml @04:34:40.68 ATV.dial :air2 @04:34:41.72 AS.reconfigure ASslow1 @04:34:41.77 AS.seek 0.32ml @04:34:46.14 ATV.dial ASV @04:34:47.17 AS.reconfigure ASslow0 @04:34:47.22 AS.seek 0.57ml @04:35:12.61 ASV.dial :wasteD @04:35:13.20 AS.reconfigure ASnorm @04:35:13.25 AS.seek 0ml @04:35:42.29 ATV.dial :air2 @04:35:43.36 AS.reconfigure ASslow0 @04:35:43.41 AS.seek 0.3ml @04:36:14.02 ATV.dial :ASV @04:36:14.97 ASV.dial :wasteD @04:36:14.99 AS.reconfigure ASconfig @04:36:15.04 AS.seek 0.05ml @04:36:29.72 ATV.dial :waste @04:36:31.53 AS.seek 0ml @04:36:34.71 ASV.dial :SPEdelivery @04:36:35.74 deconLysateINT @04:36:35.75 ATV.dial :air3 @04:36:36.42 AS.reconfigure ASslow1 @04:36:36.47 AS.seek 0.01ml @04:36:38.86 delay 2 seconds @04:36:40.89 ATV.dial :bleach @04:36:41.34 AS.seek 0.96ml @04:39:52.72 delay 3 seconds @04:39:55.75 ATV.dial :air3 @04:39:56.29 delay 3 seconds @04:39:59.38 AS.seek 0.985ml @04:40:04.72 delay 3 seconds @04:40:07.75 ATV.dial :bleach @04:40:08.36 AS.seek 0.97ml @04:40:11.80 delay 3 seconds @04:40:14.83 ATV.dial AIV @04:40:16.04 AIV.dial :coilToMFB @04:40:16.06 AWV.select :waste @04:40:16.07 AFV.select :carrierSolution @04:40:16.18 delay 2 seconds @04:40:18.22 AS.reconfigure ASconfig @04:40:18.26 AS.seek 1ml @04:40:20.29 delay 1 second @04:40:21.31 AFV.select :holdingCoil @04:40:21.41 delay 1 second @04:40:22.44 AS.reconfigure ASslow0 @04:40:22.49 AS.seek :empty @04:42:03.47 AFV.select :carrierSolution @04:42:03.58 delay 2 seconds @04:42:05.61 AS.reconfigure ASconfig @04:42:05.66 AS.seek 1ml @04:43:03.47 delay 1 second @04:43:04.49 AFV.select :holdingCoil @04:43:04.59 delay 1 second @04:43:05.62 AS.reconfigure ASslow0 @04:43:05.67 AS.seek :empty @04:44:46.66 AFV.select :carrierSolution @04:44:46.77 delay 2 seconds @04:44:48.80 AS.reconfigure ASconfig @04:44:48.85 AS.seek 1ml @04:45:46.66 delay 1 second @04:45:47.69 AFV.select :holdingCoil @04:45:47.79 delay 1 second @04:45:48.82 AS.reconfigure ASslow0 @04:45:48.87 AS.seek :empty @04:47:29.85 ATV.dial :air2 @04:47:30.51 AS.reconfigure ASslow1 @04:47:30.56 AS.seek 0.009ml @04:47:32.86 AS.reconfigure ASslow0 @04:47:32.91 AS.seek 1ml @04:49:12.88 ATV.dial AIV @04:49:13.75 AS.seek 0.02ml @04:50:52.66 ATV.dial :waste @04:50:54.83 AS.seek 0ml @04:50:57.13 AWV.select :air @04:50:57.33 Cleaning PCR @04:50:57.34 AFV.select :carrierSolution @04:50:57.45 delay 2 seconds @04:50:59.48 AS.reconfigure ASconfig @04:50:59.53 AS.seek 0.5ml @04:51:28.57 delay 1 second @04:51:29.59 AFV.select :holdingCoil @04:51:29.69 ASV.dial :waste1 @04:51:30.07 ATV.dial :air3 @04:51:30.75 AS.reconfigure ASslow1 @04:51:30.80 AS.seek 0.51ml @04:51:33.08 delay 2 seconds @04:51:35.10 ATV.dial :bleach @04:51:35.56 AS.seek 0.81ml @04:52:36.18 delay 3 seconds @04:52:39.21 ATV.dial :air3 @04:52:39.77 delay 3 seconds @04:52:42.86 AS.seek 0.835ml @04:52:48.26 delay 3 seconds @04:52:51.28 ATV.dial :bleach @04:52:51.89 AS.seek 0.82ml @04:52:55.30 delay 3 seconds @04:52:58.33 ARV.dial :waste @04:52:58.94 delay 3 seconds @04:53:01.97 ATV.dial :ARV @04:53:02.84 AS.reconfigure ASconfig @04:53:02.89 AS.seek 0.47ml @04:53:23.29 AFV.select :carrierSolution @04:53:23.40 delay 2 seconds @04:53:25.44 AS.seek 1ml @04:53:56.21 delay 1 second @04:53:57.23 AFV.select :holdingCoil @04:53:57.33 delay 1 second @04:53:58.36 AS.reconfigure ASfast @04:53:58.41 AS.seek 0ml @04:54:25.75 AFV.select :carrierSolution @04:54:25.86 delay 2 seconds @04:54:27.89 AS.reconfigure ASconfig @04:54:27.94 AS.seek 1ml @04:55:25.72 delay 1 second @04:55:26.74 AFV.select :holdingCoil @04:55:26.84 delay 1 second @04:55:27.88 AS.reconfigure ASfast @04:55:27.92 AS.seek 0ml @04:55:55.25 AFV.select :carrierSolution @04:55:55.36 delay 2 seconds @04:55:57.39 AS.reconfigure ASconfig @04:55:57.44 AS.seek 1ml @04:56:55.21 delay 1 second @04:56:56.23 AFV.select :holdingCoil @04:56:56.33 delay 1 second @04:56:57.36 AS.reconfigure ASfast @04:56:57.41 AS.seek 0ml @04:57:24.75 ARV.dial between carrier and air1 @04:57:25.24 ATV.dial :air3 @04:57:26.50 AS.reconfigure ASslow1 @04:57:26.55 AS.seek 0.01ml @04:57:28.93 delay 2 seconds @04:57:30.95 ATV.dial :bleach @04:57:31.56 AS.seek 0.81ml @05:00:12.72 delay 3 seconds @05:00:15.75 ATV.dial :air3 @05:00:16.29 delay 3 seconds @05:00:19.38 AS.seek 0.835ml @05:00:24.69 delay 3 seconds @05:00:27.72 ATV.dial :bleach @05:00:28.43 AS.seek 0.82ml @05:00:31.74 delay 3 seconds @05:00:34.77 ATV.dial PCR @05:00:35.16 AS.reconfigure ASconfig @05:00:35.21 AS.seek :empty @05:01:22.69 PCR.program :bleachProfile @05:01:22.75 PCR program should complete in < 2:45 @05:01:22.76 PCR.run 10:00 @05:04:33.67 PCR.off @05:04:33.70 AFV.select :carrierSolution @05:04:33.80 delay 2 seconds @05:04:35.84 AS.seek 1ml @05:05:33.69 delay 1 second @05:05:34.71 AFV.select :holdingCoil @05:05:34.81 delay 1 second @05:05:35.84 AS.reconfigure ASfast @05:05:35.89 AS.seek 0ml @05:06:03.79 delay 3 seconds @05:06:06.82 AFV.select :carrierSolution @05:06:06.92 delay 2 seconds @05:06:08.95 AS.reconfigure ASconfig @05:06:09.01 AS.seek 1ml @05:07:06.80 delay 1 second @05:07:07.83 AFV.select :holdingCoil @05:07:07.93 delay 1 second @05:07:08.97 AS.reconfigure ASfast @05:07:09.01 AS.seek 0ml @05:07:36.35 delay 3 seconds @05:07:39.38 AFV.select :carrierSolution @05:07:39.48 delay 2 seconds @05:07:41.51 AS.reconfigure ASconfig @05:07:41.56 AS.seek 1ml @05:08:39.30 delay 1 second @05:08:40.32 AFV.select :holdingCoil @05:08:40.42 delay 1 second @05:08:41.45 AS.reconfigure ASfast @05:08:41.50 AS.seek 0ml @05:09:08.85 delay 3 seconds @05:09:11.88 AFV.select :carrierSolution @05:09:11.98 delay 2 seconds @05:09:14.02 AS.reconfigure ASconfig @05:09:14.07 AS.seek 1ml @05:10:11.83 delay 1 second @05:10:12.86 AFV.select :holdingCoil @05:10:12.96 delay 1 second @05:10:13.99 AS.reconfigure ASfast @05:10:14.04 AS.seek 0ml @05:10:41.38 delay 3 seconds @05:10:44.41 AFV.select :carrierSolution @05:10:44.51 delay 2 seconds @05:10:46.54 AS.reconfigure ASconfig @05:10:46.60 AS.seek 1ml @05:11:44.35 delay 1 second @05:11:45.38 AFV.select :holdingCoil @05:11:45.48 delay 1 second @05:11:46.51 AS.reconfigure ASnorm @05:11:46.56 AS.seek 0ml @05:12:37.26 AFV.select :carrierSolution @05:12:37.37 delay 2 seconds @05:12:39.40 AS.reconfigure ASconfig @05:12:39.45 AS.seek 1ml @05:13:37.18 delay 1 second @05:13:38.20 AFV.select :holdingCoil @05:13:38.30 delay 1 second @05:13:39.33 ATV.dial :waste @05:13:40.62 AS.reconfigure ASnorm @05:13:40.67 AS.seek 0.5ml @05:14:06.24 ATV.dial :waste2 @05:14:07.51 AS.seek 0ml @05:14:33.06
Asleep after completing 18tun1 assays @05:14:33.08 GoodNews.email "{1 => 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 4, 7 => 6}\nCan@05:08:53, 22.6C, 53% humidity, 14.8psia, 12.060V, 0.506A, 0.506A avg, 6.10W",Subject: "Asleep after completing 18tun1 assays" @05:14:33.10
Mission runtime was 12:45:39.556208 @05:15:13.28 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @05:15:13.31 Retry #1 of 10 after 10:00 @05:15:33.35
Sending Email @05:18:33.39 Email::Error in MAIN -- flush failed! @18:05:15.21CEST14-Sep-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @18:05:17.24 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @18:05:17.91 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @18:05:23.63 Polling Can every 10:00 @18:05:25.47 SPE.coast @18:05:25.52 SPE.configure SPEconfig @18:05:25.63 AS.reconfigure ASconfig @18:05:25.68 AS.hold @18:05:25.73 AS.configure ASconfig @18:05:25.83 SS.reconfigure SSconfig @18:05:25.88 SS.hold @18:05:25.93 SS.configure SSconfig @18:05:26.03 CC.reconfigure CCconfig @18:05:26.08 CC.hold @18:05:26.14 CC.configure CCconfig @18:05:26.24 CS.reconfigure CSconfig @18:05:26.28 CS.hold @18:05:26.37 CS.configure CSconfig @18:05:26.85 PC.reconfigure PCconfig @18:05:26.89 PC.hold @18:05:26.95 PC.configure PCconfig @18:05:27.04 PS.configure PSconfig @18:05:27.16 Forearm.reconfigure LooseForearm @18:05:27.21 Forearm.hold @18:05:27.26 Forearm.configure LooseForearm @18:05:27.35 Elbow.reconfigure LooseElbow @18:05:27.40 Elbow.hold @18:05:27.47 Elbow.configure LooseElbow @18:05:27.58 SC.reconfigure SCloose @18:05:27.63 SC.hold @18:05:27.68 SC.configure SCloose @18:05:27.78 SE.reconfigure SEconfig @18:05:27.82 SE.hold @18:05:27.89 SE.configure SEconfig @18:05:27.93 CH.coast @18:05:27.98 CH.configure CHconfig @18:05:28.02 PH.coast @18:05:28.08 PH.configure PHconfig @18:05:28.11 PCR.off @18:05:28.14 PCR.pidtemp= 50,0.1,0,300,25,25 @18:05:28.18 PCR.caltemp= 2.7073,0.0373,0 @18:05:28.21 All dwarf controllers running firmware version 3.75 @18:05:28.36 Cleanly exited at 17:57:21CEST14-Sep-18 @18:05:28.40 Loading /home/esp/esp2/mission/18tun1.rb mission @18:05:28.47 Preparing to sleep until +0:00:15 @18:05:28.49 GoodNews.email "Starting 18tun1 w/pucks in tube 2\n{1 => 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 4, 7 => 6}\nCan@18:05:23, 21.8C, 54% humidity, 14.6psia, 11.933V, 0.985A, 0.985A avg, 11.76W",Subject: "18tun1 begun" @18:05:28.58 AS.coast @18:05:28.61
Asleep til +0:00:15 @18:05:28.65 Continuing at 18:05 @18:05:28.68 SS.coast @18:05:28.76 CC.coast @18:05:28.83 CS.coast @18:05:28.87 PC.coast @18:05:28.91 Forearm.coast @18:05:28.95 Elbow.coast @18:05:28.99 SC.coast @18:05:29.03 SE.coast @18:05:29.43 Gate.power :core,:OFF @18:05:29.44 Gate.power :raw,:OFF @18:05:29.45 Shutdown @18:05:36.46 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:105\n{1 => 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 4, 7 => 6}\nCan@18:05:28, 21.8C, 54% humidity, 14.6psia, 11.933V, 0.973A, 0.966A avg, 11.60W",Subject: "Asleep until 18:05" @18:05:43.45
Powering core bus @18:05:48.25 Gate.power :raw,:ON @18:05:48.92 SPE.coast @18:05:48.98 SPE.configure SPEconfig @18:05:49.09 AS.configure ASconfig @18:05:49.20 SS.configure SSconfig @18:05:49.30 CC.configure CCconfig @18:05:49.41 CS.configure CSconfig @18:05:49.51 PC.configure PCconfig @18:05:49.64 PS.configure PSconfig @18:05:49.76 Forearm.configure LooseForearm @18:05:49.88 Elbow.configure LooseElbow @18:05:50.01 SC.configure SCloose @18:05:50.13 SE.configure SEconfig @18:05:50.17 CH.coast @18:05:50.21 CH.configure CHconfig @18:05:50.25 PH.coast @18:05:50.31 PH.configure PHconfig @18:05:50.35 PCR.off @18:05:50.38 PCR.pidtemp= 50,0.1,0,300,25,25 @18:05:50.42 PCR.caltemp= 2.7073,0.0373,0 @18:05:50.45 All dwarf controllers running firmware version 3.75 @18:05:50.58 Valve.reset @18:05:50.75 PCR.off @18:05:50.79 PCR.pidtemp= 50,0.1,0,300,25,25 @18:05:50.82 PCR.caltemp= 2.7073,0.0373,0 @18:05:51.06 Intake.select :close @18:05:51.08 Exhaust.select :close @18:05:51.09 CTV.dial :air @18:05:51.19 PSV.dial :air @18:05:51.34 CSV.dial :waste1 @18:05:51.38 PS.reconfigure PSconfig @18:05:51.40
Forearm.reconfigure LooseForearm @18:05:51.57 PS.home.seek 0ml @18:05:51.59
Forearm.home.seek :retract @18:05:52.08 SSV.dial :spit,avoiding: :exhaust @18:05:52.23 CS.reconfigure CSfast @18:05:52.26 SS.reconfigure SSconfig @18:05:52.37 CS.home.seek 0ml @18:05:52.41 SS.home.seek 0ml @18:05:52.77 Hand.close @18:05:52.82
Elbow.jog 1500 @18:05:53.40 Elbow.reconfigure LooseElbow @18:05:53.44 Elbow.home.seek :clear @18:05:56.84 SE.reconfigure SEconfig @18:05:56.89 SE.home.seek :down @18:05:57.59 SC.reconfigure SCsloppy @18:05:57.69 SC.home.seek :initial @18:05:57.99 Session Begun @18:06:01.29 PS.coast @18:06:01.94
Forearm.seek :retract @18:06:02.05 Elbow.seek :hammer @18:06:03.30 Forearm.reconfigure TightForearm @18:06:03.38 Forearm.seek 5 + 350 counts @18:06:04.51 Forearm.seek :retract @18:06:05.78 PC.reconfigure PCconfig @18:06:05.80
CC.reconfigure CCconfig @18:06:05.92 PC.home.seek :open @18:06:05.94
CC.home.seek :open @18:06:09.37 Phase [mbaPhase] @18:06:09.39 GoodNews.email "At 18:06:09 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:105\n{1 => 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 4, 7 => 6}\nCan@18:06:09, 21.8C, 54% humidity, 14.6psia, 11.987V, 0.752A, 0.752A avg, 9.01W",Subject: "Started 18tun1 phase mbaPhase" @18:06:09.43CEST14-Sep-18
==================> STARTED MBAsuperphase (phase #1) <=== @18:06:09.56 Starting mfb @18:06:09.58 pcrBAC sampling at most 200ml, wcr at most 200ml @18:06:09.70 Loading sh1 puck from tube 2 into Collection Clamp @18:06:09.83 Readying the MFB @18:06:09.85
Hand.open @18:06:09.89 Homing MFB Syringe @18:06:09.94 AFV.select :holdingCoil @18:06:10.13 ATV.dial :air2 @18:06:10.99
SC.reconfigure SCloose @18:06:11.19 Elbow.reconfigure TightElbow @18:06:11.27 Elbow.seek 2 @18:06:11.58 AS.reconfigure ASconfig @18:06:11.69 AS.home.seek :home @18:06:11.96
Forearm.seek 2 + 700 counts @18:06:13.08 SE.reconfigure SEpick @18:06:13.19 SE.seek beamBreak @18:06:14.95 SE.configure SEconfig @18:06:15.16 Elbow.seek 2 @18:06:15.43 Forearm.seek 2 + 300 counts @18:06:15.75 Forearm.reconfigure LooseForearm @18:06:15.84 Hand.close @18:06:16.34 BACfans.email "Started mfb in tube 2 at 18:06:09CEST14-Sep-18\n{1 => 6, 2 => 16, 3 => 22, 4 => 22, 5 => 22, 6 => 4, 7 => 6}\nCan@18:06:09, 21.8C, 54% humidity, 14.6psia, 11.987V, 0.752A, 0.752A avg, 9.01W",Subject: "pcrBAC sampling at most 200ml, wcr at most 200ml" @18:06:16.62 SE.seek :down @18:06:16.90
Forearm.seek :retract @18:06:17.36 AFV.select :carrierSolution @18:06:17.49 delay 2 seconds @18:06:17.90
Elbow.seek CC @18:06:18.07 SE.coast @18:06:19.55
Elbow.reconfigure LooseElbow @18:06:19.69 AS.seek 1ml @18:06:19.78
Forearm.reconfigure TightForearm @18:06:19.87 Forearm.seek CC @18:06:21.22 CC.seek :closed @18:06:25.51 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.51 @18:06:25.52 Hand.open @18:06:26.42 Forearm.reconfigure LooseForearm @18:06:26.49 Forearm.seek :retract @18:06:27.47 SC.coast @18:06:27.53 collectSample 200ml w/bubble point of 55psi @18:06:27.55 Priming 7ml @18:06:27.57 Intake.select :close @18:06:27.60 Exhaust.select :close @18:06:27.63 SS.hold @18:06:27.90 Calibrating pressure and temperature sensors @18:06:27.92 CTV.dial :air @18:06:27.95 SSV.dial :spit,avoiding: :exhaust @18:06:27.99 SS.reconfigure SSfast @18:06:28.08 SS.seek 5ml @18:06:30.99 SSV.dial CBV @18:06:31.03
CBV.dial SSV @18:06:32.55 delay 3.8 seconds @18:06:36.50 SSV.dial :spit @18:06:38.03 SS.seek 0ml @18:06:40.79 SSV.dial CBV @18:06:42.29 CTV.dial :intake @18:06:43.16 SSV.dial CBV @18:06:43.18
CBV.dial SSV @18:06:43.21 Allowing 40s for 7.0ml of remaining 7.0ml @18:06:43.24 Intake.select :open @18:06:45.44 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:06:45.46 SS.reconfigure SSinhale.with maxSpeed: 150 @18:06:45.55 SS.seek 7ml @18:06:51.55 Stroke completed -- waiting for pressure to equalize ... @18:07:06.14 Intake.select :close @18:07:06.33 SSV.dial :exhaust @18:07:07.21 Exhaust.select :open @18:07:07.35 SS.reconfigure SSexhale @18:07:07.42 SS.seek 0ml @18:07:11.56 Exhaust.select :close @18:07:11.70 Sampling 200.0ml @18:07:11.72 Intake.select :close @18:07:11.74 Exhaust.select :close @18:07:11.77 SS.reconfigure SSconfig @18:07:11.85 SS.hold @18:07:12.10 CTV.dial :intake @18:07:12.13 SSV.dial CBV @18:07:12.16
CBV.dial SSV @18:07:12.60 delay 1 second @18:07:13.07
Allowing 130s for 25.0ml of remaining 200.0ml @18:07:13.09 Intake.select :open @18:07:13.62 AFV.select :holdingCoil @18:07:13.74 delay 1 second @18:07:14.77 ATV.dial :waste @18:07:15.29
Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:07:15.32 SS.reconfigure SSinhale.with maxSpeed: 150 @18:07:15.40 SS.seek 25ml @18:07:15.83 AS.reconfigure ASnorm @18:07:15.90 AS.home.seek :empty @18:07:19.39
Vacuum too high -- waiting up to 40s @18:07:26.54 Intake @15.2psia, vacuum from 8.7 to 12.7psi @18:07:26.57 SS.reconfigure SSinhale.with maxSpeed: 135 @18:07:26.65 SS.seek 25ml @18:07:30.10 Vacuum too high -- waiting up to 40s @18:07:37.83 Intake @15.4psia, vacuum from 8.9 to 12.9psi @18:07:37.85 SS.reconfigure SSinhale.with maxSpeed: 122 @18:07:37.94 SS.seek 25ml @18:07:41.70 Vacuum too high -- waiting up to 40s @18:07:49.43 Intake @15.4psia, vacuum from 8.9 to 12.9psi @18:07:49.46 SS.reconfigure SSinhale.with maxSpeed: 110 @18:07:49.54 SS.seek 25ml @18:07:53.59 Vacuum too high -- waiting up to 40s @18:08:01.55 Intake @15.3psia, vacuum from 8.8 to 12.8psi @18:08:01.57 SS.reconfigure SSinhale.with maxSpeed: 99 @18:08:02.16 SS.seek 25ml @18:08:05.84 Vacuum too high -- waiting up to 40s @18:08:08.51 ATV.dial :waste @18:08:08.53 ARV.dial between carrier and air1 @18:08:08.64 ASV.dial :waste1 @18:08:13.23
Intake @15.3psia, vacuum from 8.8 to 12.8psi @18:08:13.26 SS.reconfigure SSinhale.with maxSpeed: 89 @18:08:13.35 SS.seek 25ml @18:08:16.10 Stroke completed -- waiting for pressure to equalize ... @18:08:22.45 Intake.select :close @18:08:22.64 SSV.dial :exhaust @18:08:23.52 Exhaust.select :open @18:08:23.66 SS.reconfigure SSexhale @18:08:23.72 SS.seek 0ml @18:08:33.22 Exhaust.select :close @18:08:33.35 SSV.dial CBV @18:08:33.38
CBV.dial SSV @18:08:34.26 Allowing 130s for 25.0ml of remaining 175.0ml @18:08:34.27 Intake.select :open @18:08:36.48 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:08:36.51 SS.reconfigure SSinhale.with maxSpeed: 89 @18:08:36.60 SS.seek 25ml @18:08:41.17 Vacuum too high -- waiting up to 40s @18:08:46.89 Intake @15.4psia, vacuum from 8.9 to 12.9psi @18:08:46.92 SS.reconfigure SSinhale.with maxSpeed: 80 @18:08:47.00 SS.seek 25ml @18:08:50.94 Vacuum too high -- waiting up to 40s @18:08:56.13 Intake @15.3psia, vacuum from 8.8 to 12.8psi @18:08:56.16 SS.reconfigure SSinhale.with maxSpeed: 72 @18:08:56.25 SS.seek 25ml @18:09:01.00 Vacuum too high -- waiting up to 40s @18:09:06.39 SS.reconfigure SSinhale.with maxSpeed: 65 @18:09:06.47 SS.seek 25ml @18:09:11.81 Vacuum too high -- waiting up to 40s @18:09:17.72 Intake @15.3psia, vacuum from 8.8 to 12.8psi @18:09:17.75 SS.reconfigure SSinhale.with maxSpeed: 59 @18:09:17.83 SS.seek 25ml @18:09:24.69 Vacuum too high -- waiting up to 40s @18:09:30.12 SS.reconfigure SSinhale.with maxSpeed: 53 @18:09:30.20 SS.seek 25ml @18:09:32.62 -> exit @18:09:32.64 Session Ended @18:09:34.83
Stroke completed -- waiting for pressure to equalize ... @18:09:40.61 Intake.select :close @18:09:40.77 SSV.dial :exhaust @18:09:41.65 Exhaust.select :open @18:09:41.77 SS.reconfigure SSexhale @18:09:41.81 SS.seek 0ml @18:09:51.43 Exhaust.select :close @18:09:51.54 SSV.dial CBV @18:09:51.57
CBV.dial SSV @18:09:52.44 Allowing 130s for 25.0ml of remaining 150.0ml @18:09:52.45 Intake.select :open @18:09:54.62 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:09:54.64 SS.reconfigure SSinhale.with maxSpeed: 53 @18:09:54.69 SS.seek 25ml @18:10:02.03 Vacuum too high -- waiting up to 40s @18:10:06.14 Intake @15.2psia, vacuum from 8.7 to 12.7psi @18:10:06.16 SS.reconfigure SSinhale.with maxSpeed: 48 @18:10:06.21 SS.seek 25ml @18:10:17.03 Vacuum too high -- waiting up to 40s @18:10:21.92 Intake @15.2psia, vacuum from 8.7 to 12.7psi @18:10:21.93 SS.reconfigure SSinhale.with maxSpeed: 43 @18:10:21.98 SS.seek 25ml @18:10:38.63 Vacuum too high -- waiting up to 40s @18:10:43.46 Intake @15.1psia, vacuum from 8.6 to 12.6psi @18:10:43.47 SS.reconfigure SSinhale.with maxSpeed: 39 @18:10:43.53 SS.seek 25ml @18:10:49.24 Stroke completed -- waiting for pressure to equalize ... @18:10:52.96 Intake.select :close @18:10:53.12 SSV.dial :exhaust @18:10:53.97 Exhaust.select :open @18:10:54.09 SS.reconfigure SSexhale @18:10:54.14 SS.seek 0ml @18:11:03.36 Exhaust.select :close @18:11:03.47 SSV.dial CBV @18:11:03.50
CBV.dial SSV @18:11:04.38 Allowing 130s for 25.0ml of remaining 125.0ml @18:11:04.39 Intake.select :open @18:11:06.57 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:11:06.58 SS.reconfigure SSinhale.with maxSpeed: 39 @18:11:06.63 SS.seek 25ml @18:11:40.38 Vacuum too high -- waiting up to 40s @18:11:45.52 Intake @15.3psia, vacuum from 8.8 to 12.8psi @18:11:45.53 SS.reconfigure SSinhale.with maxSpeed: 35 @18:11:45.59 SS.seek 25ml @18:11:59.71 Stroke completed -- waiting for pressure to equalize ... @18:12:04.20 Intake.select :close @18:12:04.36 SSV.dial :exhaust @18:12:05.22 Exhaust.select :open @18:12:05.35 SS.reconfigure SSexhale @18:12:05.43 SS.seek 0ml @18:12:14.99 Exhaust.select :close @18:12:15.11 SSV.dial CBV @18:12:15.13
CBV.dial SSV @18:12:16.00 Allowing 130s for 25.0ml of remaining 100.0ml @18:12:16.01 Intake.select :open @18:12:18.19 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:12:18.20 SS.reconfigure SSinhale.with maxSpeed: 35 @18:12:18.25 SS.seek 25ml @18:13:08.67 Stroke completed -- waiting for pressure to equalize ... @18:13:13.81 Intake.select :close @18:13:13.97 SSV.dial :exhaust @18:13:14.82 Exhaust.select :open @18:13:14.94 SS.reconfigure SSexhale @18:13:14.98 SS.seek 0ml @18:13:24.57 Exhaust.select :close @18:13:24.69 SSV.dial CBV @18:13:24.71
CBV.dial SSV @18:13:25.60 Allowing 130s for 25.0ml of remaining 75.0ml @18:13:25.61 Intake.select :open @18:13:27.78 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:13:27.80 SS.reconfigure SSinhale.with maxSpeed: 35 @18:13:27.85 SS.seek 25ml @18:14:18.32 Stroke completed -- waiting for pressure to equalize ... @18:14:23.30 Intake.select :close @18:14:23.46 SSV.dial :exhaust @18:14:24.33 Exhaust.select :open @18:14:24.45 SS.reconfigure SSexhale @18:14:24.49 SS.seek 0ml @18:14:34.24 Exhaust.select :close @18:14:34.35 SSV.dial CBV @18:14:34.40
CBV.dial SSV @18:14:35.24 Allowing 130s for 25.0ml of remaining 50.0ml @18:14:35.25 Intake.select :open @18:14:37.43 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:14:37.44 SS.reconfigure SSinhale.with maxSpeed: 35 @18:14:37.49 SS.seek 25ml @18:15:27.74 Stroke completed -- waiting for pressure to equalize ... @18:15:32.85 Intake.select :close @18:15:33.01 SSV.dial :exhaust @18:15:33.86 Exhaust.select :open @18:15:33.98 SS.reconfigure SSexhale @18:15:34.02 SS.seek 0ml @18:15:43.58 Exhaust.select :close @18:15:43.69 SSV.dial CBV @18:15:43.72
CBV.dial SSV @18:15:44.60 Allowing 130s for 25.0ml of remaining 25.0ml @18:15:44.61 Intake.select :open @18:15:46.78 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:15:46.80 SS.reconfigure SSinhale.with maxSpeed: 35 @18:15:46.85 SS.seek 24.99ml @18:16:37.24 Stroke completed -- waiting for pressure to equalize ... @18:16:42.36 Intake.select :close @18:16:42.52 SSV.dial :exhaust @18:16:43.39 Exhaust.select :open @18:16:43.51 SS.reconfigure SSexhale @18:16:43.55 SS.seek 0ml @18:16:53.11 Exhaust.select :close @18:16:53.22 Sampled 200.0ml @18:16:53.24 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @18:16:53.25 Intake.select :close @18:16:53.32 CSV.dial :waste @18:16:53.35 CS.seek 0ml @18:16:53.48 SSV.dial CBV,avoiding: :exhaust @18:16:53.51
CBV.dial SSV @18:16:53.58 CSV.dial :air @18:16:54.43 CS.seek 10ml @18:16:55.64
CTV.dial :air @18:16:56.49 delay 15 seconds @18:17:11.51 SSV.dial :spit,avoiding: :exhaust @18:17:13.01 CTV.dial CSV,avoiding: :intake @18:17:13.09
SS.seek 0ml @18:17:13.16 SSV.dial CBV,avoiding: :exhaust @18:17:21.22 CBV.dial between SSV and waste2 @18:17:21.65 CTV.dial CSV,avoiding: :air @18:17:21.67
CSV.dial CTV @18:17:22.18 CS.reconfigure CSpressurize @18:17:22.23 CS.seek 0ml @18:17:48.40 CSV.dial :air @18:17:48.91 CS.reconfigure CSfast @18:17:48.96 CS.seek 10ml @18:18:15.81 CSV.dial between air and CTV @18:18:16.18 CS.seek 2.593ml @18:18:36.01 CTV.dial CSV,avoiding: :air @18:18:36.03
CSV.dial CTV @18:18:36.35 CS.reconfigure CSpressurize @18:18:36.40 CS.seek 0ml @18:18:40.80 BubblePoint: Intake @70.2psia, Outlet @14.7psia @18:18:40.81 CBV.dial SSV @18:18:41.18 SS.reconfigure SSconfig @18:18:41.23 SS.seek 10ml @18:18:47.33 delay 15 seconds @18:19:02.49 Evacuated: Intake @40.2psia, Outlet @10.4psia @18:19:02.50 SSV.dial :spit,avoiding: :exhaust @18:19:04.00 SS.reconfigure SSexhale @18:19:04.05 SS.seek 0ml @18:19:09.60 SS.reconfigure SSfast @18:19:09.65 SS.seek 5ml @18:19:12.17 SSV.dial CBV,avoiding: :exhaust @18:19:12.20
CBV.dial SSV @18:19:13.70 CTV.dial :air,avoiding: :intake @18:19:15.23 CSV.dial CTV @18:19:15.28
SS.seek 10ml @18:19:16.28 CSV.dial :air @18:19:16.79 CS.reconfigure CSfast @18:19:16.84 CS.seek 0ml @18:19:17.81
SSV.dial :spit,avoiding: :exhaust @18:19:19.31 SS.reconfigure SSexhale @18:19:19.36 SS.seek 0ml @18:19:20.97 CS.coast @18:19:24.48
SS.reconfigure SSfast @18:19:24.54 SS.seek 5ml @18:19:27.58 SSV.dial CBV,avoiding: :exhaust @18:19:27.61
CBV.dial SSV @18:19:30.14 SSV.dial :spit,avoiding: :exhaust @18:19:31.64 SS.reconfigure SSconfig @18:19:31.69 SS.seek 0ml @18:19:34.72 SS.coast @18:19:34.76 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @18:19:34.77 Intake.select :close @18:19:34.85 CSV.dial :waste @18:19:34.99 SSV.dial CBV,avoiding: :exhaust @18:19:35.02
CBV.dial SSV @18:19:35.74 CS.seek 0ml @18:19:35.81 CSV.dial :air @18:19:36.53
CTV.dial :air @18:19:36.55 delay 15 seconds @18:19:36.65 CS.seek 10ml @18:19:51.57
SSV.dial :spit,avoiding: :exhaust @18:19:53.07 CTV.dial CSV,avoiding: :intake @18:19:53.10
SS.reconfigure SSexhale @18:19:53.21 SS.seek 0ml @18:19:53.28 SSV.dial CBV,avoiding: :exhaust @18:20:03.07 CBV.dial between SSV and waste2 @18:20:03.49 CSV.dial between air and CTV @18:20:03.83 CS.seek 8.203ml @18:20:09.51 CTV.dial CSV,avoiding: :air @18:20:09.53
CSV.dial CTV @18:20:09.87 CS.reconfigure CSpressurize @18:20:09.91 CS.seek 0ml @18:20:31.43 CSV.dial :air @18:20:31.93 CS.reconfigure CSfast @18:20:31.98 CS.seek 10ml @18:20:58.31 CSV.dial between air and CTV @18:20:58.68 CS.seek 3.123ml @18:21:17.29 CTV.dial CSV,avoiding: :air @18:21:17.31
CSV.dial CTV @18:21:17.64 CS.reconfigure CSpressurize @18:21:17.69 CS.seek 0ml @18:21:26.34 BubblePoint: Intake @69.6psia, Outlet @14.3psia @18:21:26.35 CBV.dial SSV @18:21:26.71 SS.reconfigure SSconfig @18:21:26.76 SS.seek 10ml @18:21:32.27 delay 15 seconds @18:21:47.42 Evacuated: Intake @43.1psia, Outlet @14.4psia @18:21:47.43 SSV.dial :spit,avoiding: :exhaust @18:21:48.95 SS.reconfigure SSexhale @18:21:49.00 SS.seek 0ml @18:21:54.06 SS.reconfigure SSfast @18:21:54.11 SS.seek 5ml @18:21:57.24 SSV.dial CBV,avoiding: :exhaust @18:21:57.26
CBV.dial SSV @18:21:58.79 CTV.dial :air,avoiding: :intake @18:22:00.31 CSV.dial CTV @18:22:00.36
SS.seek 10ml @18:22:01.36 CSV.dial :air @18:22:01.87 CS.reconfigure CSfast @18:22:01.92 CS.seek 0ml @18:22:03.08
SSV.dial :spit,avoiding: :exhaust @18:22:03.61 CS.coast @18:22:04.59
SS.reconfigure SSexhale @18:22:04.64 SS.seek 0ml @18:22:09.71 SS.reconfigure SSfast @18:22:09.77 SS.seek 5ml @18:22:12.89 SSV.dial CBV,avoiding: :exhaust @18:22:12.91
CBV.dial SSV @18:22:15.44 SSV.dial :spit,avoiding: :exhaust @18:22:16.94 SS.reconfigure SSconfig @18:22:16.99 SS.seek 0ml @18:22:20.06 SS.coast @18:22:20.10 Lysing: Adding 1.5ml lysis @18:22:20.11 CBV.dial between CSV and waste1,avoiding: :DBV @18:22:21.78 CTV.dial CSV @18:22:23.28 CSV.dial CSR @18:22:24.17 Pulling 1ml of CSR.lysis @18:22:24.18 CSR.select :lysis @18:22:24.31 CS.reconfigure CSconfig @18:22:24.35 CS.seek 1ml @18:22:27.87 delay 5 seconds @18:22:32.90 CSR.select :air @18:22:33.08 CS.seek 6ml @18:22:46.90 delay 2 seconds @18:22:48.92 CSV.dial :waste1 @18:22:50.51 CS.reconfigure CSfast @18:22:50.56 CS.seek :empty @18:23:06.62 CSV.dial CSR @18:23:08.14 Pulling 1.5ml of CSR.lysis @18:23:08.15 CSR.select :lysis @18:23:08.28 CS.reconfigure CSconfig @18:23:08.33 CS.seek 1.5ml @18:23:13.19 delay 5 seconds @18:23:18.22 CSR.select :air @18:23:18.40 CS.seek 4.5ml @18:23:27.03 delay 2 seconds @18:23:29.05 CSV.dial CTV @18:23:29.55 CS.seek 1.275ml @18:23:38.79 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @18:23:38.81 CH.seek 85C,8:00 @18:27:11.21 CH.hold 5:00,85C @18:32:11.39 Collection Puck Heater at 80.9C after lysing @18:32:11.41 CS.seek 0ml @18:32:15.69 CTV.dial between spare and air,avoiding: :intake @18:32:16.87 CSV.dial :air @18:32:17.37 CS.seek 5ml @18:32:31.15 CSV.dial CTV @18:32:31.66 delay 2 seconds @18:32:33.69 CSV.dial :air @18:32:34.19 delay 2 seconds @18:32:36.21 CSV.dial CTV @18:32:36.72 delay 2 seconds @18:32:38.75 CSV.dial :air @18:32:39.25 delay 2 seconds @18:32:41.29 CS.seek 0ml @18:32:55.47 Recovering Lysate into Collection syringe @18:32:55.48 CSV.dial CBV @18:32:56.63 CBV.dial CSV @18:32:57.06 CS.seek 4.999ml @18:33:10.75 delay 5 seconds @18:33:15.78 CTV.dial :air @18:33:16.37 CS.seek 6.999ml @18:33:22.47 delay 5 seconds @18:33:27.50 CSV.dial :air2 @18:33:28.01 CS.seek 10ml @18:33:36.66 CSV.dial between air and CTV,avoiding: CBV @18:33:38.08 Lysing Complete @18:33:38.17 Storing sh1 puck in Collection Clamp to tube 1 @18:33:38.36 SC.reconfigure SCtight @18:33:38.41 SC.seek 1 @18:33:40.53 SC.reconfigure SCloose @18:33:40.76 Elbow.reconfigure TightElbow @18:33:41.31 Elbow.seek 1 @18:33:43.31 Forearm.reconfigure TightForearm @18:33:43.37 Forearm.seek 1 + 700 counts @18:33:44.39 SE.reconfigure SEpick @18:33:44.48 SE.seek beamBreak @18:33:47.20 SE.configure SEconfig @18:33:47.37 Elbow.reconfigure LimpElbow @18:33:47.43 Forearm.reconfigure LooseForearm @18:33:47.48 Forearm.seek 1 @18:33:47.78 Elbow.hold @18:33:47.85 Elbow.reconfigure TightElbow @18:33:47.96 SE.seek 5.09 Pucks below Top Plate @18:33:48.49 Forearm.force -20 @18:33:48.53 Forearm.seek 1 + 344 counts @18:33:48.86 Forearm.reconfigure TightForearm @18:33:48.91 Forearm.seek 1 + 700 counts @18:33:49.23 SE.seek 6.09 Pucks below Top Plate @18:33:49.90 Forearm.reconfigure LooseForearm @18:33:49.95 Forearm.seek :retract @18:33:50.89 Elbow.seek CC @18:33:52.59 Elbow.reconfigure LooseElbow @18:33:52.69 Forearm.seek CC @18:33:53.72 Hand.close @18:33:54.50 CC.seek :open @18:33:59.02 Forearm.seek :retract @18:34:00.02 Elbow.reconfigure TightElbow @18:34:00.07 Elbow.seek 1 @18:34:02.03 Elbow.reconfigure LimpElbow @18:34:02.08 Forearm.seek 1 @18:34:03.10 Elbow.hold @18:34:03.17 Elbow.reconfigure TightElbow @18:34:03.22 Hand.open @18:34:04.15 Forearm.force -20 @18:34:04.19 Forearm.seek 1 + 350 counts @18:34:04.50 Forearm.reconfigure TightForearm @18:34:04.55 Forearm.seek 1 + 700 counts @18:34:04.94 SE.seek :down @18:34:06.09
Forearm.reconfigure LooseForearm @18:34:06.14 Forearm.seek :retract @18:34:07.17 SE.coast @18:34:07.22
SC.coast @18:34:07.26 Tranferring lysate to PRV @18:34:07.27 CSV.dial :air @18:34:07.62 CS.seek 10ml @18:34:07.70 CSV.dial between air and CTV @18:34:08.05 AIV.dial :bypass @18:34:08.14 Beginning samplePassC @18:34:08.16 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:34:08.70 Hand.open @18:34:09.16 Elbow.seek FlushPuck::Garage @18:34:11.12 Elbow.reconfigure LooseElbow @18:34:11.23 Forearm.seek :garage @18:34:12.25 Hand.close @18:34:13.14 Forearm.seek :retract @18:34:14.19 Elbow.reconfigure TightElbow @18:34:14.24 Elbow.seek CC @18:34:17.17 Elbow.reconfigure LooseElbow @18:34:17.27 Forearm.reconfigure TightForearm @18:34:17.33 Forearm.seek CC @18:34:18.61 CC.seek :closed @18:34:22.88 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.36 @18:34:22.96 Hand.open @18:34:23.82 Forearm.reconfigure LooseForearm @18:34:23.87 Forearm.seek :retract @18:34:24.86 AWV.select :air @18:34:24.87 AIV.dial :bypass @18:34:24.88 PSV.dial PSR @18:34:26.42 Pulling 1.5ml of PSR.flush @18:34:26.43 PSR.select :flush @18:34:26.57 PS.seek 1.5ml @18:34:42.77 delay 5 seconds @18:34:47.80 PSV.dial :waste1 @18:34:48.32 PS.seek 0ml @18:35:04.55 PSV.dial PSR @18:35:05.06 PSR.select :air @18:35:05.19 PS.seek 2.5ml @18:35:31.61 PRV.dial CBV @18:35:31.66
CBV.dial PRV @18:35:32.55 PSV.dial :mixloop @18:35:33.83 CTV.dial :air,avoiding: :intake @18:35:33.84 PTV.dial :PRVmixing @18:35:33.93 PS.seek 0ml @18:36:00.39 PSV.dial :air @18:36:00.92 PS.seek 2.5ml @18:36:27.38 PSV.dial :mixloop @18:36:28.01 PS.seek 0ml @18:36:54.42 PSV.dial :air @18:36:54.90 PS.seek 2.5ml @18:37:21.49 PSV.dial :mixloop @18:37:22.00 PS.seek 0ml @18:37:48.58 Garaging FlushPuck in Collection Clamp @18:37:48.66 Elbow.reconfigure TightElbow @18:37:48.71 Elbow.seek CC @18:37:48.96 Elbow.reconfigure LooseElbow @18:37:49.07 Forearm.seek CC @18:37:50.04 Hand.close @18:37:50.76 CC.seek :open @18:37:55.57 Forearm.seek :retract @18:37:56.65 Elbow.reconfigure TightElbow @18:37:56.70 Elbow.seek :garage @18:37:59.91 Elbow.reconfigure LooseElbow @18:38:00.02 Forearm.reconfigure TightForearm @18:38:00.07 Forearm.seek :garage @18:38:01.26 Hand.open @18:38:02.08 Forearm.reconfigure LooseForearm @18:38:02.13 Forearm.seek :retract @18:38:03.18 Connecting PS to CS to transfer sample @18:38:03.19 CBV.dial AIV @18:38:03.20 AIV.dial :bypass @18:38:03.22 PSV.dial :mixloop @18:38:03.23 PTV.dial :PRVmixing @18:38:03.24 PRV.dial AIV @18:38:03.26 CTV.dial :air,avoiding: :intake @18:38:03.28 Loading lyfil puck from tube 2 into Collection Clamp @18:38:03.54 SC.reconfigure SCtight @18:38:03.59 SC.seek 2 @18:38:05.77 SC.reconfigure SCloose @18:38:06.00 Elbow.reconfigure TightElbow @18:38:06.05 Elbow.seek 2 @18:38:08.15 Forearm.reconfigure TightForearm @18:38:08.20 Forearm.seek 2 + 700 counts @18:38:09.20 SE.reconfigure SEpick @18:38:09.28 SE.seek beamBreak @18:38:11.11 SE.configure SEconfig @18:38:11.29 Elbow.seek 2 @18:38:11.49 Forearm.seek 2 + 300 counts @18:38:11.77 Forearm.reconfigure LooseForearm @18:38:11.82 Hand.close @18:38:12.65 SE.seek :down @18:38:12.89
Forearm.seek :retract @18:38:13.87 Elbow.seek CC @18:38:14.19 SE.coast @18:38:15.53
Elbow.reconfigure LooseElbow @18:38:15.63 Forearm.reconfigure TightForearm @18:38:15.68 Forearm.seek CC @18:38:17.00 CC.seek :closed @18:38:21.24 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.45 @18:38:21.25 Hand.open @18:38:22.09 Forearm.reconfigure LooseForearm @18:38:22.14 Forearm.seek :retract @18:38:23.16 SC.coast @18:38:23.20 CTV.dial CSV,avoiding: :intake @18:38:24.71 CSV.dial CTV @18:38:25.03 CS.seek 5ml @18:38:38.76 delay 1:20 @18:39:58.80 CS.seek 2.5ml @18:40:06.13 delay 40 seconds @18:40:46.17 CS.seek 0ml @18:40:53.51 delay 1:00 @18:41:53.54 PSV.dial :air @18:41:54.03 PS.seek 2.5ml @18:42:20.71 PSV.dial :mixloop @18:42:21.71 delay 3 seconds @18:42:24.74 PSV.dial :air @18:42:25.24 delay 2 seconds @18:42:27.27 PSV.dial :mixloop @18:42:27.75 delay 3 seconds @18:42:30.77 PSV.dial :waste1 @18:42:32.25 PS.seek :empty @18:42:58.83 CBV.dial :waste1 @18:42:59.35 delay 5 seconds @18:43:04.38 CBV.dial AIV @18:43:04.90 delay 10 seconds @18:43:14.93 CBV.dial :waste1 @18:43:15.45 delay 10 seconds @18:43:25.48 CBV.dial AIV @18:43:26.01 delay 10 seconds @18:43:36.04 CTV.dial CSV,avoiding: :intake @18:43:36.05 CBV.dial between AIV and waste1 @18:43:36.42 CSV.dial :air @18:43:36.94 CS.seek 10ml @18:44:03.42 CSV.dial CTV @18:44:03.94 CS.seek 0ml @18:44:30.60 CBV.dial :waste1 @18:44:30.93 delay 10 seconds @18:44:40.96 CTV.dial :air,avoiding: :intake @18:44:42.52 Storing lyfil puck in Collection Clamp to tube 1 @18:44:42.71 SC.reconfigure SCtight @18:44:42.76 SC.seek 1 @18:44:44.93 SC.reconfigure SCloose @18:44:45.16 Elbow.reconfigure TightElbow @18:44:45.21 Elbow.seek 1 @18:44:47.16 Forearm.reconfigure TightForearm @18:44:47.21 Forearm.seek 1 + 700 counts @18:44:48.29 SE.reconfigure SEpick @18:44:48.37 SE.seek beamBreak @18:44:51.08 SE.configure SEconfig @18:44:51.26 Elbow.reconfigure LimpElbow @18:44:51.31 Forearm.reconfigure LooseForearm @18:44:51.36 Forearm.seek 1 @18:44:51.66 Elbow.hold @18:44:51.73 Elbow.reconfigure TightElbow @18:44:51.84 SE.seek 6.08 Pucks below Top Plate @18:44:52.44 Forearm.force -20 @18:44:52.48 Forearm.seek 1 + 343 counts @18:44:52.80 Forearm.reconfigure TightForearm @18:44:52.85 Forearm.seek 1 + 700 counts @18:44:53.17 SE.seek 7.08 Pucks below Top Plate @18:44:53.92 Forearm.reconfigure LooseForearm @18:44:53.97 Forearm.seek :retract @18:44:54.91 Elbow.seek CC @18:44:56.65 Elbow.reconfigure LooseElbow @18:44:56.75 Forearm.seek CC @18:44:57.73 Hand.close @18:44:58.52 CC.seek :open @18:45:03.05 Forearm.seek :retract @18:45:04.06 Elbow.reconfigure TightElbow @18:45:04.11 Elbow.seek 1 @18:45:06.05 Elbow.reconfigure LimpElbow @18:45:06.10 Forearm.seek 1 @18:45:07.58 Elbow.hold @18:45:07.73 Elbow.reconfigure TightElbow @18:45:07.78 Hand.open @18:45:08.66 Forearm.force -20 @18:45:08.70 Forearm.seek 1 + 346 counts @18:45:09.03 Forearm.reconfigure TightForearm @18:45:09.08 Forearm.seek 1 + 700 counts @18:45:09.42 SE.seek :down @18:45:10.58
Forearm.reconfigure LooseForearm @18:45:10.63 Forearm.seek :retract @18:45:11.58 SE.coast @18:45:11.62
SC.coast @18:45:11.66 PSV.dial :air @18:45:12.91 PS.seek 0.499ml @18:45:18.90 delay 3 seconds @18:45:21.92 PSV.dial :mixloop @18:45:22.41 PRV.dial :air @18:45:22.93 delay 3 seconds @18:45:25.96 PRV.dial CBV @18:45:26.49 CBV.dial AIV @18:45:27.01 PRV.dial between CBV and air @18:45:27.36 PRV.dial :air @18:45:27.76 PS.reconfigure PSslow @18:45:27.81 PS.seek 0.599ml @18:45:30.40 PRV.dial :waste2 @18:45:31.22 PS.reconfigure PSconfig @18:45:31.27 PS.seek :empty @18:45:38.27 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:45:38.35 Hand.open @18:45:38.77 Elbow.seek FlushPuck::Garage @18:45:40.73 Elbow.reconfigure LooseElbow @18:45:40.83 Forearm.seek :garage @18:45:41.88 Hand.close @18:45:42.78 Forearm.seek :retract @18:45:43.84 Elbow.reconfigure TightElbow @18:45:43.89 Elbow.seek CC @18:45:46.81 Elbow.reconfigure LooseElbow @18:45:46.92 Forearm.reconfigure TightForearm @18:45:46.97 Forearm.seek CC @18:45:48.31 CC.seek :closed @18:45:52.57 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.32 @18:45:52.65 Hand.open @18:45:53.46 Forearm.reconfigure LooseForearm @18:45:53.51 Forearm.seek :retract @18:45:54.54 Clearing PML for modified lysate @18:45:54.55 CTV.dial :air,avoiding: :intake @18:45:54.56 PRV.dial :waste2 @18:45:54.58 PTV.dial :PRV,avoiding: :puck @18:45:55.41 delay 2 seconds @18:45:57.43 PTV.dial :PRVmixing,avoiding: :puck @18:45:58.77 delay 2 seconds @18:46:00.80 PSV.dial :PML @18:46:00.81 delay 2 seconds @18:46:02.84 PRV.dial :air @18:46:03.68 delay 2 seconds @18:46:05.77 PS.seek 1.499ml @18:46:22.08 delay 5 seconds @18:46:27.12 PS.seek 2.5ml @18:46:38.15 PSV.dial :air @18:46:38.67 PS.seek 0ml @18:47:05.27 mixing lysate with speDil @18:47:05.28 PSV.dial :PML @18:47:05.79 CBV.dial :PRV @18:47:05.80 PRV.dial :air @18:47:05.82 delay 2 seconds @18:47:07.84 PRV.dial :speDil @18:47:08.37 delay 2 seconds @18:47:10.47 PS.reconfigure PSslow1 @18:47:10.52 PS.seek 0.225ml @18:47:34.77 delay 5 seconds @18:47:39.80 PRV.dial CBV @18:47:40.66 delay 2 seconds @18:47:42.75 PS.seek 0.475ml @18:48:08.65 delay 5 seconds @18:48:13.68 PRV.dial :air @18:48:14.19 delay 2 seconds @18:48:16.23 PS.seek 0.965ml @18:49:06.24 delay 10 seconds @18:49:16.27 PRV.dial CBV @18:49:16.77 CTV.dial :air,avoiding: :intake @18:49:16.78 PSV.dial :air @18:49:17.29 PS.reconfigure PSconfig @18:49:17.34 PS.seek 2.5ml @18:49:33.74 delay 3 seconds @18:49:36.76 PSV.dial PTV @18:49:37.60 PTV.dial :PRV,avoiding: :puck @18:49:38.44 PS.seek 0ml @18:50:04.93 PSV.dial :air @18:50:05.78 PS.seek 2.5ml @18:50:32.30 delay 3 seconds @18:50:35.32 PSV.dial PTV @18:50:36.16 PTV.dial :PRV,avoiding: :puck @18:50:36.18 PS.seek 0ml @18:51:02.90 Pushing modified lysate into AIV Injection Loop @18:51:02.91 CBV.dial AIV @18:51:02.92 AIV.dial :bypass @18:51:02.94 PRV.dial CBV @18:51:02.95 PSV.dial :air @18:51:03.80 PS.seek 2.5ml @18:51:30.21 delay 3 seconds @18:51:33.23 PSV.dial :PML @18:51:33.74 PTV.dial :PRVmixing,avoiding: :puck @18:51:34.64 PS.reconfigure PSslow1 @18:51:34.69 PS.seek 1.74ml @18:52:52.45 delay 5 seconds @18:52:57.49 AIV.dial :sample @18:52:58.12 AWV.select :waste @18:52:58.29 PS.reconfigure PSslow2 @18:52:58.34 PS.seek 1.22ml @18:54:47.02 delay 15 seconds @18:55:02.05 AIV.dial :bypass @18:55:02.67 PS.reconfigure PSconfig @18:55:02.72 PS.seek 0ml @18:55:16.02 PSV.dial :air @18:55:16.53 PS.seek 2.5ml @18:55:42.76 PSV.dial :PML @18:55:43.28 PS.seek 0ml @18:56:09.87 AIV.dial :coilToMFB @18:56:09.88 AWV.select :air @18:56:10.05 Extracting Nucleic Acid from AIV @18:56:10.08
Beginning samplePassD @18:56:10.11 SPE.hold 2:46:40,55C @18:56:10.20 ASV.dial :SPEdelivery @18:56:10.28
AWV.select :air @18:56:10.30 AIV.dial :bypass @18:56:10.31 PSV.dial PSR @18:56:10.63 delay 20 seconds @18:56:11.61
Pulling 1.5ml of PSR.killMFB @18:56:11.62 PSR.select :killMFB @18:56:11.76 PS.seek 1.5ml @18:56:28.87 delay 5 seconds @18:56:31.15 primeLysateINT @18:56:31.16 AWV.select :air @18:56:31.18 AIV.dial :coilToMFB @18:56:31.19 ATV.dial AIV @18:56:33.07 AS.reconfigure ASslow1 @18:56:33.12 AS.seek 0.299ml @18:56:33.90
PSV.dial :waste1 @18:56:34.44 PS.seek 0ml @18:56:51.49 PSV.dial PSR @18:56:52.02 PSR.select :air @18:56:52.15 PS.seek 2.5ml @18:57:19.87 PRV.dial CBV @18:57:19.90
CBV.dial PRV @18:57:19.92 PSV.dial :mixloop @18:57:21.13 CTV.dial :air,avoiding: :intake @18:57:21.14 PTV.dial :PRVmixing @18:57:21.17 PS.seek 0ml @18:57:33.86 delay 10 seconds @18:57:43.90 ATV.dial :air2 @18:57:44.79 AS.seek 0.329ml @18:57:49.18
PSV.dial PSR @18:57:50.47 Pulling 1.5ml of PSR.flush @18:57:50.48 PSR.select :flush @18:57:50.62 PS.seek 1.5ml @18:57:51.10 ATV.dial :waste2 @18:57:51.69 AS.reconfigure ASnorm @18:57:51.75 AS.seek :empty @18:58:07.71
delay 5 seconds @18:58:08.75 Lysate primed! @18:58:08.76 AFV.select :holdingCoil @18:58:08.77 ATV.dial :air2 @18:58:09.43 AS.reconfigure ASslow1 @18:58:09.48 AS.seek 0.069ml @18:58:12.74
PSV.dial :waste1 @18:58:13.28 PS.seek 0ml @18:58:23.96 delay 3 seconds @18:58:26.99 ATV.dial :AIV @18:58:27.87 AS.seek 0.469ml @18:58:30.34
PSV.dial PSR @18:58:30.88 PSR.select :air @18:58:31.01 PS.seek 2.5ml @18:58:58.68 PRV.dial CBV @18:58:58.71
CBV.dial PRV @18:58:58.72 PSV.dial :mixloop @18:58:59.93 CTV.dial :air,avoiding: :intake @18:58:59.94 PTV.dial :PRVmixing @18:58:59.97 PS.seek 0ml @18:59:27.65 PSV.dial PSR @18:59:28.92 Pulling 1.5ml of PSR.flush @18:59:28.93 PSR.select :flush @18:59:29.06 PS.seek 1.5ml @18:59:45.81 delay 5 seconds @18:59:48.58 delay 5 seconds @18:59:50.84
PSV.dial :waste1 @18:59:51.49 PS.seek 0ml @18:59:53.66 Solid Phase Extraction Heater at 49C @18:59:53.67 ATV.dial ASV @18:59:54.07 ASV.dial :SPEdelivery @18:59:54.16 AS.seek 0.041ml @19:00:08.28
PSV.dial PSR @19:00:08.92 PSR.select :air @19:00:09.05 PS.seek 2.5ml @19:00:36.15 PRV.dial CBV @19:00:36.18
CBV.dial PRV @19:00:36.19 PSV.dial :mixloop @19:00:37.40 CTV.dial :air,avoiding: :intake @19:00:37.41 PTV.dial :PRVmixing @19:00:37.44 PS.seek 0ml @19:01:04.53 PSV.dial PSR @19:01:05.77 Pulling 1.5ml of PSR.flush @19:01:05.78 PSR.select :flush @19:01:05.92 PS.seek 1.5ml @19:01:20.55 delay 10 seconds @19:01:22.53
delay 5 seconds @19:01:27.56 PSV.dial :waste1 @19:01:28.58 PS.seek 0ml @19:01:30.58 rinseLysateLineINT @19:01:30.59 AFV.select :carrierSolution @19:01:30.70 delay 2 seconds @19:01:32.73 AS.reconfigure ASconfig @19:01:32.79 AS.seek 1ml @19:01:45.05
PSV.dial PSR @19:01:45.59 PSR.select :air @19:01:45.72 PS.seek 2.5ml @19:02:12.53 PRV.dial CBV @19:02:12.56
CBV.dial PRV @19:02:12.58 PSV.dial :mixloop @19:02:13.74 CTV.dial :air,avoiding: :intake @19:02:13.75 PTV.dial :PRVmixing @19:02:13.78 PS.reconfigure PSslow @19:02:13.82 PS.seek 1.2ml @19:02:28.19 delay 1 second @19:02:29.21 AFV.select :holdingCoil @19:02:29.31 delay 1 second @19:02:30.34 ATV.dial AIV @19:02:30.89 AIV.dial :coilToMFB @19:02:30.90 AWV.select :waste @19:02:31.03 AS.reconfigure ASslow0 @19:02:31.07 AS.seek 0ml @19:02:40.65
PSV.dial :waste1 @19:02:42.18 PS.reconfigure PSconfig @19:02:42.22 PS.seek 0ml @19:02:55.59 PSV.dial :air @19:02:56.89 PS.seek 2.5ml @19:03:23.68 PSV.dial :mixloop @19:03:24.19 PS.reconfigure PSslow @19:03:24.24 PS.seek 0ml @19:04:11.99 ATV.dial :air2 @19:04:12.67 AS.reconfigure ASslow1 @19:04:12.72 AS.seek 0.05ml @19:04:15.56
PSV.dial :air @19:04:16.08 PS.reconfigure PSconfig @19:04:16.13 PS.seek 2.5ml @19:04:23.07 ATV.dial :AIV @19:04:23.90 AS.seek 0.01ml @19:04:32.36 ATV.dial :waste @19:04:34.11 AS.seek :empty @19:04:36.66 ethanol wash @19:04:36.67 ATV.dial :air2 @19:04:37.94 AS.reconfigure ASslow2 @19:04:38.00 AS.seek 0.009ml @19:04:42.91
PSV.dial :mixloop @19:04:43.43 PS.reconfigure PSslow @19:04:43.48 PS.seek 0ml @19:04:48.39 delay 3 seconds @19:04:51.42 Pulling 0.4ml of Ethanol Wash @19:04:51.43 ATV.dial :ethanol @19:04:52.28 AS.reconfigure ASslow1 @19:04:52.33 AS.seek 0.409ml @19:05:34.81
Cleaning Collection Syringe @19:05:34.82 CSV.dial CSR @19:05:35.39 Pulling 4ml of CSR.flush @19:05:35.40 CSR.select :flush @19:05:35.53 CS.seek 4ml @19:05:46.77 delay 5 seconds @19:05:51.80 CSR.select :air @19:05:51.93 CS.seek 10ml @19:06:08.30 CSV.dial CTV @19:06:08.33
CTV.dial CSV @19:06:09.91 CBV.dial :waste1 @19:06:10.44 CS.seek 0ml @19:06:13.08 delay 3 seconds @19:06:16.11 ATV.dial :air1 @19:06:16.49 delay 3 seconds @19:06:19.58 AS.seek 0.434ml @19:06:24.92 delay 3 seconds @19:06:27.95 ATV.dial :ethanol @19:06:28.65 AS.seek 0.419ml @19:06:31.96 delay 3 seconds @19:06:34.99 ATV.dial ASV @19:06:36.31 AS.seek 0.092ml @19:06:37.00
CSV.dial CSR @19:06:37.58 Pulling 4ml of CSR.flush CSR.select :flush @19:06:37.71 CS.seek 4ml @19:06:49.68 delay 5 seconds @19:06:54.71 CSR.select :air @19:06:54.84 CS.seek 10ml @19:07:11.12 CSV.dial CTV @19:07:11.15
CTV.dial CSV @19:07:11.66 CBV.dial :waste1 @19:07:11.68 CS.seek 0ml @19:07:38.31 Garaging FlushPuck in Collection Clamp @19:07:38.39 Elbow.reconfigure TightElbow @19:07:38.44 Elbow.seek CC @19:07:38.72 Elbow.reconfigure LooseElbow @19:07:38.82 Forearm.seek CC @19:07:39.82 Hand.close @19:07:40.55 CC.seek :open @19:07:42.33 delay 3 seconds @19:07:44.91
Forearm.seek :retract @19:07:45.35 ATV.dial :waste2 @19:07:45.92
Elbow.reconfigure TightElbow @19:07:45.97 Elbow.seek :garage @19:07:46.11 AS.reconfigure ASconfig @19:07:46.17 AS.seek :empty @19:07:49.05
Elbow.reconfigure LooseElbow @19:07:49.16 Forearm.reconfigure TightForearm @19:07:49.21 Forearm.seek :garage @19:07:50.41 Hand.open @19:07:51.24 Forearm.reconfigure LooseForearm @19:07:51.29 Forearm.seek :retract @19:07:51.82 ATV.dial :air2 @19:07:52.22 AS.reconfigure ASslow1 @19:07:52.27 AS.seek 1ml @19:07:52.33 Whole Cell Archive sampling 200ml @19:07:52.37 Loading wcr puck from tube 2 into Collection Clamp @19:07:52.66 SC.reconfigure SCtight @19:07:52.71 SC.seek 2 @19:07:54.88 SC.reconfigure SCloose @19:07:55.12 Elbow.reconfigure TightElbow @19:07:55.17 Elbow.seek 2 @19:07:57.24 Forearm.reconfigure TightForearm @19:07:57.29 Forearm.seek 2 + 700 counts @19:07:58.36 SE.reconfigure SEpick @19:07:58.45 SE.seek beamBreak @19:08:00.35 SE.configure SEconfig @19:08:00.54 Elbow.seek 2 @19:08:00.76 Forearm.seek 2 + 300 counts @19:08:01.04 Forearm.reconfigure LooseForearm @19:08:01.09 Hand.close @19:08:01.81 SE.seek :down @19:08:02.04 Forearm.seek :retract @19:08:03.03 Elbow.seek CC @19:08:03.48 SE.coast @19:08:04.74 Elbow.reconfigure LooseElbow @19:08:04.86 Forearm.reconfigure TightForearm @19:08:04.91 Forearm.seek CC @19:08:06.80 CC.seek :closed @19:08:11.11 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.53 @19:08:11.12 Hand.open @19:08:11.99 Forearm.reconfigure LooseForearm @19:08:12.05 Forearm.seek :retract @19:08:13.07 SC.coast @19:08:13.13 collectSample 200ml w/bubble point of 55psi @19:08:13.14 Priming 7ml @19:08:13.15 Intake.select :close @19:08:13.16 Exhaust.select :close @19:08:13.18 SS.hold @19:08:13.41 Calibrating pressure sensors @19:08:13.43 CTV.dial :air @19:08:14.93 SSV.dial :spit,avoiding: :exhaust @19:08:14.96 SS.reconfigure SSfast @19:08:15.00 SS.seek 5ml @19:08:17.94 SSV.dial CBV @19:08:17.97 CBV.dial SSV @19:08:19.55 delay 3.8 seconds @19:08:23.50 SSV.dial :spit @19:08:25.02 SS.seek 0ml @19:08:27.61 SSV.dial CBV @19:08:29.13 CTV.dial :intake @19:08:29.99 SSV.dial CBV @19:08:30.03 CBV.dial SSV @19:08:30.05 Allowing 40s for 7.0ml of remaining 7.0ml @19:08:30.06 Intake.select :open @19:08:32.24 Intake @15.7psia, vacuum from 9.2 to 13.2psi @19:08:32.26 SS.reconfigure SSinhale.with maxSpeed: 150 @19:08:32.31 SS.seek 7ml @19:08:38.31 Stroke completed -- waiting for pressure to equalize ... @19:08:56.06 Intake.select :close @19:08:56.23 SSV.dial :exhaust @19:08:57.10 Exhaust.select :open @19:08:57.23 SS.reconfigure SSexhale @19:08:57.28 SS.seek 0ml @19:09:01.47 Exhaust.select :close @19:09:01.58 Sampling 200.0ml @19:09:01.60 Intake.select :close @19:09:01.61 Exhaust.select :close @19:09:01.63 SS.reconfigure SSconfig @19:09:01.67 SS.hold @19:09:01.91 CTV.dial :intake @19:09:01.93 SSV.dial CBV @19:09:01.95 CBV.dial SSV @19:09:02.81 Allowing 130s for 25.0ml of remaining 200.0ml @19:09:02.82 Intake.select :open @19:09:05.00 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:09:05.02 SS.reconfigure SSinhale.with maxSpeed: 150 @19:09:05.08 SS.seek 25ml @19:09:09.48 Vacuum too high -- waiting up to 40s @19:09:21.56 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:09:21.58 SS.reconfigure SSinhale.with maxSpeed: 135 @19:09:21.64 SS.seek 25ml @19:09:25.87 Vacuum too high -- waiting up to 40s @19:09:38.81 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:09:38.83 SS.reconfigure SSinhale.with maxSpeed: 122 @19:09:38.88 SS.seek 25ml @19:09:43.24 Vacuum too high -- waiting up to 40s @19:09:55.78 Intake @15.4psia, vacuum from 8.9 to 12.9psi @19:09:55.80 SS.reconfigure SSinhale.with maxSpeed: 110 @19:09:55.85 SS.seek 25ml @19:10:00.37 Vacuum too high -- waiting up to 40s @19:10:13.64 SS.reconfigure SSinhale.with maxSpeed: 99 @19:10:13.69 SS.seek 25ml @19:10:15.78 Stroke completed -- waiting for pressure to equalize ... @19:10:24.72 Intake.select :close @19:10:24.88 SSV.dial :exhaust @19:10:25.75 Exhaust.select :open @19:10:25.87 SS.reconfigure SSexhale @19:10:25.93 SS.seek 0ml @19:10:35.05 Exhaust.select :close @19:10:35.16 SSV.dial CBV @19:10:35.19 CBV.dial SSV @19:10:36.63 Allowing 130s for 25.0ml of remaining 175.0ml @19:10:36.65 Intake.select :open @19:10:38.83 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:10:38.85 SS.reconfigure SSinhale.with maxSpeed: 99 @19:10:38.90 SS.seek 25ml @19:10:44.15 Vacuum too high -- waiting up to 40s @19:10:51.29 Intake @15.3psia, vacuum from 8.8 to 12.8psi @19:10:51.31 SS.reconfigure SSinhale.with maxSpeed: 89 @19:10:51.36 SS.seek 25ml @19:10:55.96 Vacuum too high -- waiting up to 40s @19:11:04.29 Intake @15.4psia, vacuum from 8.9 to 12.9psi @19:11:04.31 SS.reconfigure SSinhale.with maxSpeed: 80 @19:11:04.36 SS.seek 25ml @19:11:09.33 Vacuum too high -- waiting up to 40s @19:11:13.93 delay 5 seconds @19:11:17.43 SS.reconfigure SSinhale.with maxSpeed: 72 @19:11:17.49 SS.seek 25ml @19:11:18.97 ATV.dial ASV @19:11:19.98 ASV.dial :SPEdelivery @19:11:20.01 AS.seek 0.02ml @19:11:22.80 Vacuum too high -- waiting up to 40s @19:11:31.22 SS.reconfigure SSinhale.with maxSpeed: 65 @19:11:31.27 SS.seek 25ml @19:11:37.66 Stroke completed -- waiting for pressure to equalize ... @19:11:49.80 Intake.select :close @19:11:49.97 SSV.dial :exhaust @19:11:50.83 Exhaust.select :open @19:11:50.95 SS.reconfigure SSexhale @19:11:51.00 SS.seek 0ml @19:12:00.58 Exhaust.select :close @19:12:00.70 SSV.dial CBV @19:12:00.72 CBV.dial SSV @19:12:01.59 Allowing 130s for 25.0ml of remaining 150.0ml @19:12:01.60 Intake.select :open @19:12:03.79 Intake @15.4psia, vacuum from 8.9 to 12.9psi @19:12:03.81 SS.reconfigure SSinhale.with maxSpeed: 65 @19:12:03.86 SS.seek 25ml @19:12:09.76 Vacuum too high -- waiting up to 40s @19:12:15.04 Intake @15.3psia, vacuum from 8.8 to 12.8psi @19:12:15.06 SS.reconfigure SSinhale.with maxSpeed: 59 @19:12:15.11 SS.seek 25ml @19:12:22.12 Vacuum too high -- waiting up to 40s @19:12:28.65 Intake @15.3psia, vacuum from 8.8 to 12.8psi @19:12:28.67 SS.reconfigure SSinhale.with maxSpeed: 53 @19:12:28.72 SS.seek 25ml @19:12:37.99 Vacuum too high -- waiting up to 40s @19:12:45.33 Intake @15.3psia, vacuum from 8.8 to 12.8psi @19:12:45.35 SS.reconfigure SSinhale.with maxSpeed: 48 @19:12:45.40 SS.seek 25ml @19:12:56.33 Vacuum too high -- waiting up to 40s @19:13:04.15 Intake @15.2psia, vacuum from 8.7 to 12.7psi @19:13:04.17 SS.reconfigure SSinhale.with maxSpeed: 43 @19:13:04.22 SS.seek 25ml @19:13:06.59 Stroke completed -- waiting for pressure to equalize ... @19:13:11.76 Intake.select :close @19:13:11.92 SSV.dial :exhaust @19:13:12.78 Exhaust.select :open @19:13:12.91 SS.reconfigure SSexhale @19:13:12.96 SS.seek 0ml @19:13:22.17 Exhaust.select :close @19:13:22.28 SSV.dial CBV @19:13:22.31 CBV.dial SSV @19:13:23.19 Allowing 130s for 25.0ml of remaining 125.0ml @19:13:23.20 Intake.select :open @19:13:25.39 Intake @15.4psia, vacuum from 8.9 to 12.9psi @19:13:25.41 SS.reconfigure SSinhale.with maxSpeed: 43 @19:13:25.46 SS.seek 25ml @19:13:39.43 Vacuum too high -- waiting up to 40s @19:13:44.96 Intake @15.2psia, vacuum from 8.7 to 12.7psi @19:13:44.98 SS.reconfigure SSinhale.with maxSpeed: 39 @19:13:45.03 SS.seek 25ml @19:14:02.21 Vacuum too high -- waiting up to 40s @19:14:09.23 Intake @15.3psia, vacuum from 8.8 to 12.8psi @19:14:09.25 SS.reconfigure SSinhale.with maxSpeed: 35 @19:14:09.31 SS.seek 25ml @19:14:25.24 Stroke completed -- waiting for pressure to equalize ... @19:14:34.77 Intake.select :close @19:14:34.94 SSV.dial :exhaust @19:14:35.81 Exhaust.select :open @19:14:35.94 SS.reconfigure SSexhale @19:14:35.99 SS.seek 0ml @19:14:37.36 delay 5 seconds @19:14:42.39 ATV.dial :air2 @19:14:43.57 AS.seek 0.52ml @19:14:45.24 Exhaust.select :close @19:14:45.37 SSV.dial CBV @19:14:45.39 CBV.dial SSV @19:14:46.28 Allowing 130s for 25.0ml of remaining 100.0ml @19:14:46.30 Intake.select :open @19:14:48.47 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:14:48.49 SS.reconfigure SSinhale.with maxSpeed: 35 @19:14:48.54 SS.seek 25ml @19:15:10.68 Vacuum too high -- waiting up to 40s @19:15:17.72 Intake @15.3psia, vacuum from 8.8 to 12.8psi @19:15:17.74 SS.reconfigure SSinhale.with maxSpeed: 32 @19:15:17.79 SS.seek 25ml @19:15:43.61 Vacuum too high -- waiting up to 40s @19:15:52.25 Intake @15.3psia, vacuum from 8.8 to 12.8psi @19:15:52.27 SS.reconfigure SSinhale.with maxSpeed: 29 @19:15:52.33 SS.seek 25ml @19:15:59.58 Stroke completed -- waiting for pressure to equalize ... @19:16:07.32 Intake.select :close @19:16:07.48 SSV.dial :exhaust @19:16:08.36 Exhaust.select :open @19:16:08.48 SS.reconfigure SSexhale @19:16:08.53 SS.seek 0ml @19:16:18.11 Exhaust.select :close @19:16:18.23 SSV.dial CBV @19:16:18.25 CBV.dial SSV @19:16:19.14 Allowing 130s for 25.0ml of remaining 75.0ml @19:16:19.16 Intake.select :open @19:16:21.33 Intake @15.4psia, vacuum from 8.9 to 12.9psi @19:16:21.35 SS.reconfigure SSinhale.with maxSpeed: 29 @19:16:21.40 SS.seek 25ml @19:16:24.41 delay 5 seconds @19:16:29.44 ATV.dial ASV @19:16:30.44 ASV.dial :wasteR @19:16:30.84 AS.seek 0.3ml @19:16:51.92 Vacuum too high -- waiting up to 40s @19:16:59.50 Intake @15.3psia, vacuum from 8.8 to 12.8psi @19:16:59.52 SS.reconfigure SSinhale.with maxSpeed: 26 @19:16:59.57 SS.seek 25ml @19:17:15.41 delay 20 seconds @19:17:33.47 Stroke completed -- waiting for pressure to equalize ... @19:17:35.44 AS.seek 0.07ml @19:17:45.63 Intake.select :close @19:17:45.79 SSV.dial :exhaust @19:17:46.66 Exhaust.select :open @19:17:46.78 SS.reconfigure SSexhale @19:17:46.83 SS.seek 0ml @19:17:56.42 Exhaust.select :close @19:17:56.54 SSV.dial CBV @19:17:56.56 CBV.dial SSV @19:17:57.45 Allowing 130s for 25.0ml of remaining 50.0ml @19:17:57.46 Intake.select :open @19:17:59.64 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:17:59.66 SS.reconfigure SSinhale.with maxSpeed: 26 @19:17:59.71 SS.seek 25ml @19:18:22.02 delay 20 seconds @19:18:39.95 Vacuum too high -- waiting up to 40s @19:18:42.04 ATV.dial :waste2 @19:18:42.82 AS.reconfigure ASconfig @19:18:42.87 AS.seek :empty @19:18:47.31 Elution @19:18:47.33 Intake @15.3psia, vacuum from 8.8 to 12.8psi @19:18:47.34 ATV.dial :air1 @19:18:47.38 SS.reconfigure SSinhale.with maxSpeed: 23 @19:18:47.48 SS.seek 25ml @19:18:48.54 AS.reconfigure ASslow1 @19:18:48.59 AS.seek 0.049ml @19:18:59.00 delay 3 seconds @19:19:02.03 ATV.dial :ElutionWater @19:19:02.73 AS.seek 0.109ml @19:19:15.13 delay 3 seconds @19:19:18.16 ATV.dial ASV,via: :air2 @19:19:18.92 Stroke completed -- waiting for pressure to equalize ... @19:19:20.30 ASV.dial :SPEelution @19:19:20.91 AFV.select :carrierSolution @19:19:21.07 AS.reconfigure ASnorm @19:19:21.13 AS.seek 0.759ml @19:19:29.10 Intake.select :close @19:19:29.27 SSV.dial :exhaust @19:19:30.15 Exhaust.select :open @19:19:30.27 SS.reconfigure SSexhale @19:19:30.32 SS.seek 0ml @19:19:39.90 Exhaust.select :close @19:19:40.02 SSV.dial CBV @19:19:40.04 CBV.dial SSV @19:19:40.92 Allowing 130s for 25.0ml of remaining 25.0ml @19:19:40.93 Intake.select :open @19:19:43.12 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:19:43.14 SS.reconfigure SSinhale.with maxSpeed: 23 @19:19:43.19 SS.seek 24.99ml @19:19:54.19 delay 3 seconds @19:19:57.22 AFV.select :holdingCoil @19:19:57.38 AS.reconfigure ASslow1 @19:19:57.43 AS.seek 0.474ml @19:20:55.03 delay 5 seconds @19:20:59.61 Stroke completed -- waiting for pressure to equalize ... @19:21:00.12 AS.reconfigure ASslow2 @19:21:00.17 AS.seek 0.439ml @19:21:13.65 Intake.select :close @19:21:13.82 SSV.dial :exhaust @19:21:14.67 Exhaust.select :open @19:21:14.79 SS.reconfigure SSexhale @19:21:14.84 SS.seek 0ml @19:21:24.45 Exhaust.select :close @19:21:24.56 Sampled 200.0ml @19:21:24.58 Drying puck before fixing for 4:00 @19:21:24.59 CBV.dial :waste2b @19:21:25.45 delay 1:00 @19:21:35.63 delay 1:00 @19:22:25.49 CSV.dial :air @19:22:26.00 CS.reconfigure CSfast @19:22:26.05 CS.seek 10ml @19:22:35.66 pushing eluate to holding coil @19:22:35.74 AS.seek 0.279ml @19:22:52.53 Pumping wcr puck from tube 2 up to 55psi... @19:22:52.55 CBV.dial SSV @19:22:53.98 CBV.dial between CSV and waste2b @19:22:55.09 CSV.dial between air and CTV @19:22:55.46 CS.seek 7.315ml @19:23:03.66 CTV.dial CSV,avoiding: :air @19:23:03.69 CSV.dial CTV @19:23:04.59 CS.reconfigure CSpressurize @19:23:04.64 CS.seek 0ml @19:23:20.65 CBV.dial :waste2b @19:23:21.02 CS.reconfigure CSconfig @19:23:21.07 CS.seek :empty @19:23:25.60 delay 1:00 @19:24:25.63 CTV.dial :air,avoiding: :intake @19:24:27.14 Fix #1 of 2 (with rnal) for 2:00 @19:24:27.15 CSV.dial CSR @19:24:27.70 Pulling 2ml of CSR.rnal @19:24:27.72 CSR.select :rnal @19:24:27.84 CS.seek 2ml @19:24:34.19 delay 5 seconds @19:24:39.22 CSR.select :air @19:24:39.34 CS.reconfigure CSfast @19:24:39.40 CS.seek 10ml @19:25:00.82 Pumping wcr puck from tube 2 up to 55psi... @19:25:00.84 CBV.dial SSV @19:25:02.23 CBV.dial between CSV and waste2b @19:25:03.32 CSV.dial between air and CTV @19:25:04.67 CS.seek 7.223ml @19:25:12.94 CTV.dial CSV,avoiding: :air @19:25:12.97 CSV.dial CTV @19:25:14.58 CS.reconfigure CSpressurize @19:25:14.63 CS.seek 0ml @19:25:16.77 delay 30 seconds @19:25:32.37 delay 2:00 @19:25:46.80 ATV.dial :waste,via: :air3 @19:25:48.52 AS.reconfigure ASconfig @19:25:48.58 AS.seek :empty @19:26:04.94 Parking Bleach in LysateSource line and SPEcolumn @19:26:04.95 ATV.dial :air3 @19:26:05.79 AS.reconfigure ASslow1 @19:26:05.84 AS.seek 0.029ml @19:26:12.19 ATV.dial :ElutionWater @19:26:12.82 AS.seek 0.059ml @19:26:19.10 ATV.dial :air3 @19:26:19.73 AS.seek 0.069ml @19:26:22.02 delay 2 seconds @19:26:24.04 ATV.dial :bleach @19:26:24.49 AS.seek 0.769ml @19:27:32.40 CBV.dial :waste2b @19:27:32.77 CS.reconfigure CSconfig @19:27:32.83 CS.seek :empty @19:27:35.19 delay 1:00 @19:28:35.22 CTV.dial :air,avoiding: :intake @19:28:36.72 Fix #2 of 2 (with rnal) for 2:00 @19:28:36.74 CSV.dial CSR @19:28:37.30 Pulling 2ml of CSR.rnal @19:28:37.32 CSR.select :rnal @19:28:37.44 CS.seek 2ml @19:28:43.54 delay 5 seconds @19:28:45.58 delay 3 seconds @19:28:48.57 CSR.select :air @19:28:48.63 ATV.dial :air3 @19:28:48.70 CS.reconfigure CSfast @19:28:48.75 CS.seek 10ml @19:28:49.19 delay 3 seconds @19:28:52.28 AS.seek 0.794ml @19:28:57.60 delay 3 seconds @19:29:00.63 ATV.dial :bleach @19:29:01.24 AS.seek 0.779ml @19:29:04.47 delay 3 seconds @19:29:07.50 ATV.dial :AIV @19:29:08.75 AS.reconfigure ASconfig @19:29:08.81 AS.seek 0.015ml @19:29:10.07 Pumping wcr puck from tube 2 up to 55psi... @19:29:10.09 CBV.dial SSV @19:29:11.50 CBV.dial between CSV and waste2b @19:29:12.61 CSV.dial between air and CTV @19:29:13.31 CS.seek 7.184ml @19:29:21.48 CTV.dial CSV,avoiding: :air @19:29:21.50 CSV.dial CTV @19:29:23.08 CS.reconfigure CSpressurize @19:29:23.14 CS.seek 0ml @19:29:40.03 delay 2:00 @19:29:53.00 ATV.dial :waste @19:29:54.47 AS.seek :empty @19:29:55.67 ATV.dial :air3 @19:29:56.34 AS.reconfigure ASslow1 @19:29:56.39 AS.seek 0.009ml @19:29:58.75 delay 2 seconds @19:30:00.77 ATV.dial :bleach @19:30:01.21 AS.seek 0.579ml @19:31:40.06 CBV.dial :waste2b @19:31:40.43 CS.reconfigure CSconfig @19:31:40.48 CS.seek :empty @19:31:43.53 delay 1:00 @19:31:56.64 delay 3 seconds @19:31:59.67 ATV.dial :air3 @19:32:00.22 delay 3 seconds @19:32:03.31 AS.seek 0.604ml @19:32:08.64 delay 3 seconds @19:32:11.67 ATV.dial :bleach @19:32:12.32 AS.seek 0.589ml @19:32:15.72 delay 3 seconds @19:32:18.75 ATV.dial :ASV @19:32:19.78 ASV.dial :wasteR @19:32:20.47 AS.seek 0ml @19:32:43.56 CTV.dial :air,avoiding: :intake @19:32:45.14 Storing wcr puck in Collection Clamp to tube 1 @19:32:45.34 SC.reconfigure SCtight @19:32:45.39 SC.seek 1 @19:32:47.52 SC.reconfigure SCloose @19:32:47.76 Elbow.reconfigure TightElbow @19:32:47.82 Elbow.seek 1 @19:32:49.77 Forearm.reconfigure TightForearm @19:32:49.82 Forearm.seek 1 + 700 counts @19:32:50.89 SE.reconfigure SEpick @19:32:50.98 SE.seek beamBreak @19:32:53.58 SE.configure SEconfig @19:32:53.76 Elbow.reconfigure LimpElbow @19:32:53.81 Forearm.reconfigure LooseForearm @19:32:53.86 Forearm.seek 1 @19:32:54.18 Elbow.hold @19:32:54.26 Elbow.reconfigure TightElbow @19:32:54.37 SE.seek 7.08 Pucks below Top Plate @19:32:54.91 Forearm.force -20 @19:32:54.96 Forearm.seek 1 + 347 counts @19:32:55.30 Forearm.reconfigure TightForearm @19:32:55.36 Forearm.seek 1 + 700 counts @19:32:55.69 SE.seek 8.08 Pucks below Top Plate @19:32:56.35 Forearm.reconfigure LooseForearm @19:32:56.41 Forearm.seek :retract @19:32:57.35 Elbow.seek CC @19:32:59.07 Elbow.reconfigure LooseElbow @19:32:59.19 Forearm.seek CC @19:33:00.18 Hand.close @19:33:00.95 CC.seek :open @19:33:05.50 Forearm.seek :retract @19:33:06.48 Elbow.reconfigure TightElbow @19:33:06.54 Elbow.seek 1 @19:33:08.45 Elbow.reconfigure LimpElbow @19:33:09.05 Forearm.seek 1 @19:33:10.07 Elbow.hold @19:33:10.15 Elbow.reconfigure TightElbow @19:33:10.20 Hand.open @19:33:11.10 Forearm.force -20 @19:33:11.14 Forearm.seek 1 + 346 counts @19:33:11.48 Forearm.reconfigure TightForearm @19:33:11.54 Forearm.seek 1 + 700 counts @19:33:11.90 SE.seek :down @19:33:13.05 Forearm.reconfigure LooseForearm @19:33:13.11 Forearm.seek :retract @19:33:13.88 SE.coast @19:33:14.06 SC.coast @19:33:14.11 Starting finalPurge @19:33:14.12 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:33:14.20 Hand.open @19:33:14.60 Elbow.seek FlushPuck::Garage @19:33:16.75 Elbow.reconfigure LooseElbow @19:33:16.86 Forearm.seek :garage @19:33:17.88 Hand.close @19:33:18.71 Forearm.seek :retract @19:33:19.77 Elbow.reconfigure TightElbow @19:33:19.82 Elbow.seek CC @19:33:22.77 Elbow.reconfigure LooseElbow @19:33:22.87 Forearm.reconfigure TightForearm @19:33:22.92 Forearm.seek CC @19:33:24.24 CC.seek :closed @19:33:28.51 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.30 @19:33:28.60 Hand.open @19:33:29.39 Forearm.reconfigure LooseForearm @19:33:29.44 Forearm.seek :retract @19:33:30.43 Flushing the Collection Valve::Manifold @19:33:30.57 CSV.dial CSR @19:33:31.14 Pulling 5ml of CSR.flush CSR.select :flush @19:33:31.27 CS.seek 5ml @19:33:45.04 delay 5 seconds @19:33:50.07 CSR.select :air @19:33:50.25 CS.reconfigure CSfast @19:33:50.30 CS.seek 7.5ml @19:33:57.70 CSV.dial :waste @19:33:59.16 CS.reconfigure CSconfig @19:33:59.21 CS.seek 0ml @19:34:19.25 delay 1:00 @19:34:19.38
Flushing lower manifold's waste1 port @19:34:19.52 CSV.dial :flush @19:34:20.57 CS.reconfigure CSfast @19:34:20.62 CS.seek 5ml @19:34:34.32 delay 5 seconds @19:34:39.35 CSV.dial :air @19:34:39.93 CS.seek 7ml @19:34:45.98 CSV.dial CTV @19:34:46.01
CTV.dial CSV @19:34:47.60 CBV.dial :waste1 @19:34:48.49 CS.seek 0ml @19:35:07.35 Flushing lower manifold's waste2b port @19:35:07.49 CSV.dial :flush @19:35:08.33 CS.seek 5ml @19:35:19.28 AFV.select :carrierSolution @19:35:19.38 delay 2 seconds @19:35:21.41 AS.reconfigure ASconfig @19:35:21.46 AS.seek 1ml @19:35:22.13
delay 5 seconds @19:35:27.15 CSV.dial :air @19:35:27.86 CS.seek 7ml @19:35:33.98 CSV.dial CTV @19:35:34.01
CTV.dial CSV @19:35:34.54 CBV.dial :waste2b @19:35:35.41 CS.seek 0ml @19:35:54.17 Flushing lower manifold's waste1 port @19:35:54.18 CSV.dial CSR @19:35:54.75 Pulling 4ml of CSR.mfbkill @19:35:54.76 CSR.select :mfbkill @19:35:54.88 CS.reconfigure CSconfig @19:35:54.93 CS.seek 4ml @19:36:06.10 delay 5 seconds @19:36:11.13 CSR.select :air @19:36:11.25 CS.reconfigure CSfast @19:36:11.30 CS.seek 10ml @19:36:19.22 delay 1 second @19:36:20.23 AFV.select :holdingCoil @19:36:20.34 delay 1 second @19:36:21.37 ATV.dial ASV @19:36:21.38 ASV.dial :wasteR @19:36:21.40 AS.reconfigure ASslow1 @19:36:21.45 AS.seek 0ml @19:36:27.60 CSV.dial CTV @19:36:27.63
CTV.dial CSV @19:36:28.15 CBV.dial :waste1 @19:36:29.02 CS.seek 0ml @19:36:55.57 Flushing lower manifold's waste2b port @19:36:55.58 CSV.dial CSR @19:36:56.15 Pulling 4ml of CSR.mfbkill @19:36:56.16 CSR.select :mfbkill @19:36:56.29 CS.reconfigure CSconfig @19:36:56.34 CS.seek 4ml @19:37:07.54 delay 5 seconds @19:37:12.57 CSR.select :air @19:37:12.69 CS.reconfigure CSfast @19:37:12.74 CS.seek 10ml @19:37:29.01 CSV.dial CTV @19:37:29.04
CTV.dial CSV @19:37:29.56 CBV.dial :waste2b @19:37:30.43 CS.seek 0ml @19:37:56.92 Killing upper manifold @19:37:56.93 CSV.dial CSR @19:37:57.51 Pulling 4ml of CSR.mfbkill @19:37:57.52 CSR.select :mfbkill @19:37:57.65 CS.reconfigure CSconfig @19:37:57.70 CS.seek 4ml @19:38:09.01 delay 5 seconds @19:38:14.04 CSR.select :air @19:38:14.17 CS.reconfigure CSfast @19:38:14.21 CS.seek 10ml @19:38:30.35 CSV.dial CBV @19:38:30.38
CBV.dial CSV @19:38:31.59 CTV.dial :air @19:38:33.11 CS.seek 0ml @19:38:59.64 delay 7 seconds @19:39:06.68 CS.seek 3ml @19:39:15.54 CSV.dial :waste @19:39:16.04 CS.seek 0ml @19:39:24.57 Flushing upper manifold @19:39:24.71 CSV.dial :flush @19:39:25.21 CS.seek 5ml @19:39:38.95 delay 5 seconds @19:39:43.36 delay 20 seconds @19:39:43.97
CSV.dial :air @19:39:44.55 CS.seek 7ml @19:39:50.79 CSV.dial CBV @19:39:50.82
CBV.dial CSV @19:39:52.00 CTV.dial :air @19:39:52.02 CS.seek 0ml @19:40:03.39 ATV.dial :air3 @19:40:04.90 AS.seek 0.59ml @19:40:10.73
delay 7 seconds @19:40:17.77 CS.seek 3ml @19:40:26.45 CSV.dial :waste @19:40:26.97 CS.seek 0ml @19:40:35.67 CSV.dial :flush @19:40:36.18 CS.seek 3ml @19:40:44.79 delay 5 seconds @19:40:49.82 CSV.dial :air @19:40:50.39 CS.seek 5ml @19:40:56.55 CSV.dial :air @19:40:56.60 CS.seek 0ml @19:41:10.17 delay 7 seconds @19:41:17.21 CS.seek 3ml @19:41:25.92 CSV.dial :waste @19:41:26.77 CS.seek 0ml @19:41:35.33 Flushing lower manifold's waste1 port @19:41:35.47 CSV.dial :flush @19:41:35.99 CS.seek 5ml @19:41:49.76 delay 5 seconds @19:41:54.79 CSV.dial :air @19:41:55.36 CS.seek 7ml @19:42:01.45 CSV.dial CTV @19:42:01.48
CTV.dial CSV @19:42:03.08 CBV.dial :waste1 @19:42:03.60 CS.seek 0ml @19:42:03.85 delay 3 seconds @19:42:06.87 ATV.dial :ASV @19:42:08.30 ASV.dial :wasteR @19:42:08.39 AS.seek 0.09ml @19:42:22.33
Flushing lower manifold's waste2b port @19:42:22.47 CSV.dial :flush @19:42:23.32 CS.seek 5ml @19:42:37.11 delay 5 seconds @19:42:42.15 CSV.dial :air @19:42:42.72 CS.seek 7ml @19:42:48.79 CSV.dial CTV @19:42:48.82
CTV.dial CSV @19:42:49.33 CBV.dial :waste2b @19:42:50.19 CS.seek 0ml @19:43:08.98 Garaging FlushPuck in Collection Clamp @19:43:09.06 Elbow.reconfigure TightElbow @19:43:09.11 Elbow.seek CC @19:43:09.37 Elbow.reconfigure LooseElbow @19:43:09.47 Forearm.seek CC @19:43:10.46 Hand.close @19:43:11.21 CC.seek :open @19:43:15.61 Forearm.seek :retract @19:43:16.61 Elbow.reconfigure TightElbow @19:43:16.66 Elbow.seek :garage @19:43:19.76 Elbow.reconfigure LooseElbow @19:43:19.86 Forearm.reconfigure TightForearm @19:43:19.91 Forearm.seek :garage @19:43:21.09 Hand.open @19:43:22.44 Forearm.reconfigure LooseForearm @19:43:22.57 Forearm.seek :retract @19:43:23.64 Starting finalPurge @19:43:23.65 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:43:23.73 Hand.open @19:43:24.03 Elbow.reconfigure TightElbow @19:43:24.08 Elbow.seek FlushPuck::Garage @19:43:24.23 Elbow.reconfigure LooseElbow @19:43:24.34 Forearm.seek :garage @19:43:25.39 Hand.close @19:43:26.23 Forearm.seek :retract @19:43:27.25 Elbow.reconfigure TightElbow @19:43:27.30 Elbow.seek CC @19:43:30.14 Elbow.reconfigure LooseElbow @19:43:30.24 Forearm.reconfigure TightForearm @19:43:30.29 Forearm.seek CC @19:43:31.60 CC.seek :closed @19:43:35.87 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.22 @19:43:35.95 Hand.open @19:43:36.79 Forearm.reconfigure LooseForearm @19:43:36.84 Forearm.seek :retract @19:43:37.85 Flushing the Collection Valve::Manifold @19:43:37.99 CSV.dial CSR @19:43:38.54 Pulling 5ml of CSR.flush @19:43:38.55 CSR.select :flush @19:43:38.68 CS.reconfigure CSconfig @19:43:38.73 CS.seek 5ml @19:43:49.16 delay 30 seconds @19:43:52.55
delay 5 seconds @19:43:57.58 CSR.select :air @19:43:57.76 CS.reconfigure CSfast @19:43:57.81 CS.seek 7.501ml @19:44:05.17 CSV.dial :waste @19:44:06.75 CS.reconfigure CSconfig @19:44:06.80 CS.seek 0ml @19:44:19.19 ATV.dial :extSrc @19:44:19.96 delay 3 seconds @19:44:22.99 ATV.dial :ElutionWater @19:44:24.10 AS.seek 0.12ml @19:44:27.20
Flushing lower manifold's waste1 port @19:44:27.33 CSV.dial :flush @19:44:27.84 CS.reconfigure CSfast @19:44:27.89 CS.seek 5ml @19:44:30.42 ATV.dial :air3 @19:44:31.04 AS.seek 0.13ml @19:44:33.39 delay 2 seconds @19:44:35.41 ATV.dial :bleach @19:44:35.85 AS.seek 0.23ml @19:44:41.55
delay 5 seconds @19:44:46.58 CSV.dial :air @19:44:47.15 CS.seek 7ml @19:44:53.20 CSV.dial CTV @19:44:53.22
CTV.dial CSV @19:44:53.73 CBV.dial :waste1 @19:44:54.59 CS.seek 0ml @19:44:56.27 delay 3 seconds @19:44:59.30 ATV.dial :air3 @19:44:59.85 delay 3 seconds @19:45:02.94 AS.seek 0.255ml @19:45:08.16 delay 3 seconds @19:45:11.19 ATV.dial :bleach @19:45:11.81 AS.seek 0.24ml @19:45:13.28
Flushing lower manifold's waste2b port @19:45:13.42 CSV.dial :flush @19:45:14.82 CS.seek 5ml @19:45:15.13 delay 3 seconds @19:45:18.15 ATV.dial :ASV @19:45:19.19 ASV.dial :SPEdelivery @19:45:19.58 AFV.select :carrierSolution @19:45:19.69 delay 2 seconds @19:45:21.72 AS.reconfigure ASconfig @19:45:21.77 AS.seek 0.5ml @19:45:28.61
delay 5 seconds @19:45:33.64 CSV.dial :air @19:45:34.36 CS.seek 7ml @19:45:37.02 delay 1 second @19:45:38.04 AFV.select :holdingCoil @19:45:38.20 AS.reconfigure ASslow1 @19:45:38.25 AS.seek 0.17ml @19:45:40.42 CSV.dial CTV @19:45:40.45
CTV.dial CSV @19:45:40.97 CBV.dial :waste2b @19:45:41.84 CS.seek 0ml @19:46:00.48 Disinfecting external Intake with kill @19:46:00.62 CSV.dial CSR @19:46:01.18 Pulling 8ml of CSR.kill CSR.select :kill @19:46:01.31 CS.seek 8ml @19:46:22.70 delay 5 seconds @19:46:27.73 CSR.select :air @19:46:27.86 CS.seek 10ml @19:46:33.92 CSV.dial CBV @19:46:33.95
CBV.dial CSV @19:46:35.14 CTV.dial :intake @19:46:36.00 Intake.select :open @19:46:36.12 CS.seek 2ml @19:46:44.86 SPE.hold 2:46:40,95C @19:46:44.92 delay 3:00 @19:46:58.54
Intake.select :close @19:46:58.66 CTV.dial :air @19:46:59.55 CS.reconfigure CSconfig @19:46:59.60 CS.seek 0ml @19:47:05.83 CSV.dial CSR @19:47:07.11 Pulling 5ml of CSR.flush @19:47:07.12 CSR.select :flush @19:47:07.25 CS.seek 5ml @19:47:21.45 delay 5 seconds @19:47:26.48 CSR.select :air @19:47:26.61 CS.seek 10ml @19:47:40.82 CSV.dial CBV @19:47:42.06 CS.seek 0ml @19:48:09.58 Garaging FlushPuck in Collection Clamp @19:48:09.66 Elbow.reconfigure TightElbow @19:48:09.71 Elbow.seek CC @19:48:09.94 Elbow.reconfigure LooseElbow @19:48:10.04 Forearm.seek CC @19:48:11.01 Hand.close @19:48:11.72 CC.seek :open @19:48:16.12 Forearm.seek :retract @19:48:17.10 Elbow.reconfigure TightElbow @19:48:17.15 Elbow.seek :garage @19:48:20.36 Elbow.reconfigure LooseElbow @19:48:20.46 Forearm.reconfigure TightForearm @19:48:20.51 Forearm.seek :garage @19:48:21.70 Hand.open @19:48:22.53 Forearm.reconfigure LooseForearm @19:48:22.58 Forearm.seek :retract @19:48:23.65 Completed [mbaPhase] begun 18:06:09 @19:48:23.66 Extending 18tun1 until completion of MFBPCR @19:49:44.96 AFV.select :carrierSolution @19:49:45.07 delay 2 seconds @19:49:47.10 AS.reconfigure ASconfig @19:49:47.15 AS.seek 1ml @19:50:35.17 delay 1 second @19:50:36.19 AFV.select :holdingCoil @19:50:36.29 delay 1 second @19:50:37.32 ASV.dial :wasteD @19:50:38.42 AS.seek 0ml @19:51:36.19 Priming Eluate @19:51:36.20 AFV.select :carrierSolution @19:51:36.31 delay 2 seconds @19:51:38.35 AS.seek 0.5ml @19:52:07.38 delay 1 second @19:52:08.40 AFV.select :holdingCoil @19:52:08.50 ATV.dial ASV @19:52:08.51 ASV.dial :SPErecovery @19:52:09.11 Swish #1 of 2 @19:52:09.19 AS.reconfigure ASslow0 @19:52:09.24 AS.seek 0.6ml @19:52:19.64 AS.seek 0.5ml @19:52:30.06 Swish #2 of 2 @19:52:30.15 AS.seek 0.6ml @19:52:41.20 AS.seek 0.5ml @19:52:51.58 delay 3 seconds @19:52:54.67 AS.reconfigure ASslow1 @19:52:54.73 AS.seek 0.684ml @19:53:32.16 delay 3 seconds @19:53:35.19 Eluate primed @19:53:35.20 ATV.dial :waste2 @19:53:35.83 AS.reconfigure ASnorm @19:53:35.88 AS.seek 0ml @19:54:10.67 SPE.coast @19:54:10.76 ASV.dial :SPErecovery @19:54:10.78 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep1419h200ml.pcr @19:54:10.79 Building PCR Slug from ASV with MM2 mix, PP4 primer @19:54:10.81 Priming with carrier @19:54:10.82 ATV.dial ARV @19:54:11.78 ARV.dial :carrier @19:54:12.14 AS.seek 0.5ml @19:54:37.71 AFV.select :carrierSolution @19:54:37.82 delay 2 seconds @19:54:39.85 AS.reconfigure ASconfig @19:54:39.90 AS.seek 1ml @19:55:08.92 delay 1 second @19:55:09.94 AFV.select :holdingCoil @19:55:10.04 delay 1 second @19:55:11.07 ATV.dial :waste @19:55:12.55 AS.reconfigure ASnorm @19:55:12.61 AS.seek :empty @19:56:03.33 AFV.select :carrierSolution @19:56:03.44 delay 2 seconds @19:56:05.47 AS.reconfigure ASconfig @19:56:05.52 AS.seek 0.5ml @19:56:34.60 delay 1 second @19:56:35.62 AFV.select :holdingCoil @19:56:35.72 Building Slug @19:56:35.73 ATV.dial :ARV @19:56:37.32 delay 2 seconds @19:56:39.35 ARV.dial :air1 @19:56:39.86 delay 2 seconds @19:56:41.95 AS.reconfigure ASslow2 @19:56:42.00 AS.seek 0.506ml @19:56:48.33 delay 2 seconds @19:56:50.36 ARV.dial :MM2 @19:56:51.88 delay 2 seconds @19:56:53.91 Adding MM2 @19:56:54.00 AS.seek 0.524ml @19:57:12.21 delay 25 seconds @19:57:37.24 ARV.dial :PP4 @19:57:37.64 Adding PP4 @19:57:37.66 delay 2 seconds @19:57:39.74 AS.seek 0.53ml @19:57:46.08 delay 10 seconds @19:57:56.11 ARV.dial :air3 @19:57:56.50 adding ARV air @19:57:56.52 delay 2 seconds @19:57:58.60 AS.seek 0.536ml @19:58:04.83 delay 5 seconds @19:58:09.86 ARV.dial :carrier @19:58:11.14 delay 7 seconds @19:58:18.23 AS.seek 0.594ml @19:59:16.77 delay 5 seconds @19:59:21.80 ATV.dial :ASV @19:59:22.19 delay 2 seconds @19:59:24.28 AS.seek 0.6ml @19:59:30.57 delay 4 seconds @19:59:34.60 ATV.dial ARV @19:59:35.14 delay 2 seconds @19:59:37.17 ARV.dial :carrier @19:59:37.18 delay 2 seconds @19:59:39.28 AS.seek 0.65ml @20:00:30.00 delay 4 seconds @20:00:34.03 ATV.dial PCR @20:00:34.63 ASV.dial :waste1 @20:00:35.42 ARV.dial :air1 @20:00:35.80 Positioning Slug by 0.324ml @20:00:35.89 AS.seek 0.326ml @20:06:01.85 delay 5 seconds @20:06:06.88 ATV.dial ARV @20:06:07.64 ASV.dial :PCRlock @20:06:08.02 delay 5 seconds @20:06:13.05 Running Tt1 Profile60C300 thermocycling @20:06:13.06 PCR.program Profile60C300 @20:06:13.20 Profile60C300 should complete in < 1:45:21 @20:06:13.21 PCR.run 2:16:41.25 @21:29:37.74 PCR.off @21:29:39.29 PCR for Tt1 completed -- closed /var/log/esp/Tt118sep1419h200ml.pcr @21:29:39.36 Cleaning PCR @21:29:39.37 AFV.select :carrierSolution @21:29:39.48 delay 2 seconds @21:29:41.51 AS.reconfigure ASconfig @21:29:41.56 AS.seek 0.5ml @21:29:51.86 delay 1 second @21:29:53.45 AFV.select :holdingCoil @21:29:53.55 ASV.dial :waste1 @21:29:53.94 ATV.dial :air3 @21:29:55.23 AS.reconfigure ASslow1 @21:29:55.28 AS.seek 0.51ml @21:29:57.58 delay 2 seconds @21:29:59.60 ATV.dial :bleach @21:30:00.23 AS.seek 0.81ml @21:31:00.86 delay 3 seconds @21:31:03.89 ATV.dial :air3 @21:31:04.42 delay 3 seconds @21:31:07.51 AS.seek 0.835ml @21:31:12.98 delay 3 seconds @21:31:16.01 ATV.dial :bleach @21:31:16.62 AS.seek 0.82ml @21:31:19.85 delay 3 seconds @21:31:22.88 ARV.dial :waste @21:31:23.50 delay 3 seconds @21:31:26.53 ATV.dial :ARV @21:31:27.42 AS.reconfigure ASconfig @21:31:27.47 AS.seek 0.47ml @21:31:47.89 AFV.select :carrierSolution @21:31:48.00 delay 2 seconds @21:31:50.04 AS.seek 1ml @21:32:20.83 delay 1 second @21:32:21.85 AFV.select :holdingCoil @21:32:21.95 delay 1 second @21:32:22.98 AS.reconfigure ASfast @21:32:23.03 AS.seek 0ml @21:32:50.38 AFV.select :carrierSolution @21:32:50.49 delay 2 seconds @21:32:52.52 AS.reconfigure ASconfig @21:32:52.57 AS.seek 1ml @21:33:50.32 delay 1 second @21:33:51.34 AFV.select :holdingCoil @21:33:51.44 delay 1 second @21:33:52.47 AS.reconfigure ASfast @21:33:52.52 AS.seek 0ml @21:34:19.87 AFV.select :carrierSolution @21:34:19.99 delay 2 seconds @21:34:22.02 AS.reconfigure ASconfig @21:34:22.07 AS.seek 1ml @21:35:19.83 delay 1 second @21:35:20.85 AFV.select :holdingCoil @21:35:20.95 delay 1 second @21:35:21.98 AS.reconfigure ASfast @21:35:22.03 AS.seek 0ml @21:35:49.38 ARV.dial between carrier and air1 @21:35:49.90 ATV.dial :air3 @21:35:51.17 AS.reconfigure ASslow1 @21:35:51.22 AS.seek 0.01ml @21:35:53.58 delay 2 seconds @21:35:55.60 ATV.dial :bleach @21:35:56.21 AS.seek 0.81ml @21:38:37.36 delay 3 seconds @21:38:40.39 ATV.dial :air3 @21:38:40.92 delay 3 seconds @21:38:44.01 AS.seek 0.835ml @21:38:49.33 delay 3 seconds @21:38:52.36 ATV.dial :bleach @21:38:52.98 AS.seek 0.82ml @21:38:56.44 delay 3 seconds @21:38:59.47 ATV.dial PCR @21:38:59.86 AS.reconfigure ASconfig @21:38:59.91 AS.seek :empty @21:39:47.39 PCR.program :bleachProfile @21:39:47.45 PCR program should complete in < 2:45 @21:39:47.46 PCR.run 10:00 @21:42:43.22 PCR.off @21:42:43.26 AFV.select :carrierSolution @21:42:43.37 delay 2 seconds @21:42:45.41 AS.seek 1ml @21:43:43.24 delay 1 second @21:43:44.26 AFV.select :holdingCoil @21:43:44.36 delay 1 second @21:43:45.39 AS.reconfigure ASfast @21:43:45.44 AS.seek 0ml @21:44:12.78 delay 3 seconds @21:44:15.81 AFV.select :carrierSolution @21:44:15.91 delay 2 seconds @21:44:17.94 AS.reconfigure ASconfig @21:44:18.00 AS.seek 1ml @21:45:15.78 delay 1 second @21:45:16.80 AFV.select :holdingCoil @21:45:16.90 delay 1 second @21:45:17.93 AS.reconfigure ASfast @21:45:17.98 AS.seek 0ml @21:45:45.31 delay 3 seconds @21:45:48.34 AFV.select :carrierSolution @21:45:48.44 delay 2 seconds @21:45:50.96 AS.reconfigure ASconfig @21:45:51.10 AS.seek 1ml @21:46:48.90 delay 1 second @21:46:49.93 AFV.select :holdingCoil @21:46:50.03 delay 1 second @21:46:51.06 AS.reconfigure ASfast @21:46:51.11 AS.seek 0ml @21:47:18.49 delay 3 seconds @21:47:21.52 AFV.select :carrierSolution @21:47:21.62 delay 2 seconds @21:47:23.65 AS.reconfigure ASconfig @21:47:23.72 AS.seek 1ml @21:48:21.53 delay 1 second @21:48:22.55 AFV.select :holdingCoil @21:48:22.65 delay 1 second @21:48:23.68 AS.reconfigure ASfast @21:48:23.73 AS.seek 0ml @21:48:51.08 delay 3 seconds @21:48:54.11 AFV.select :carrierSolution @21:48:54.21 delay 2 seconds @21:48:56.24 AS.reconfigure ASconfig @21:48:56.29 AS.seek 1ml @21:49:54.07 delay 1 second @21:49:55.09 AFV.select :holdingCoil @21:49:55.19 delay 1 second @21:49:56.22 AS.reconfigure ASnorm @21:49:56.27 AS.seek 0ml @21:50:47.01 Setting up PCR for Rp -- writing /var/log/esp/Rp18sep1421h200ml.pcr @21:50:47.03 Building PCR Slug from ASV with MM2 mix, PP3 primer @21:50:47.05 Priming with carrier @21:50:47.06 ATV.dial ARV @21:50:47.66 ARV.dial :carrier @21:50:48.04 AS.seek 0.5ml @21:51:13.61 AFV.select :carrierSolution @21:51:13.72 delay 2 seconds @21:51:15.75 AS.reconfigure ASconfig @21:51:15.80 AS.seek 1ml @21:51:44.88 delay 1 second @21:51:45.90 AFV.select :holdingCoil @21:51:46.00 delay 1 second @21:51:47.03 ATV.dial :waste @21:51:48.66 AS.reconfigure ASnorm @21:51:48.71 AS.seek :empty @21:52:39.44 AFV.select :carrierSolution @21:52:39.55 delay 2 seconds @21:52:41.58 AS.reconfigure ASconfig @21:52:41.63 AS.seek 0.5ml @21:53:10.74 delay 1 second @21:53:11.76 AFV.select :holdingCoil @21:53:11.86 Building Slug @21:53:11.87 ATV.dial :ARV @21:53:13.57 delay 2 seconds @21:53:15.60 ARV.dial :air1 @21:53:16.01 delay 2 seconds @21:53:18.10 AS.reconfigure ASslow2 @21:53:18.15 AS.seek 0.506ml @21:53:24.55 delay 2 seconds @21:53:26.57 ARV.dial :MM2 @21:53:28.07 delay 2 seconds @21:53:30.10 Adding MM2 @21:53:30.18 AS.seek 0.524ml @21:53:48.47 delay 25 seconds @21:54:13.50 ARV.dial :PP3 @21:54:13.89 Adding PP3 @21:54:13.91 delay 2 seconds @21:54:15.99 AS.seek 0.53ml @21:54:22.27 delay 10 seconds @21:54:32.30 ARV.dial :air2 @21:54:32.69 adding ARV air @21:54:32.70 delay 2 seconds @21:54:34.79 AS.seek 0.536ml @21:54:41.14 delay 5 seconds @21:54:46.17 ARV.dial :carrier @21:54:47.46 delay 7 seconds @21:54:54.55 AS.seek 0.594ml @21:55:53.11 delay 5 seconds @21:55:58.14 ATV.dial :ASV @21:55:58.55 delay 2 seconds @21:56:00.64 AS.seek 0.6ml @21:56:07.07 delay 4 seconds @21:56:11.10 ATV.dial ARV @21:56:11.64 delay 2 seconds @21:56:13.67 ARV.dial :carrier @21:56:13.68 delay 2 seconds @21:56:15.77 AS.seek 0.65ml @21:57:06.32 delay 4 seconds @21:57:10.35 ATV.dial PCR @21:57:10.96 ASV.dial :waste1 @21:57:10.97 ARV.dial :air1 @21:57:11.39 Positioning Slug by 0.324ml @21:57:11.48 AS.seek 0.326ml @22:02:37.58 delay 5 seconds @22:02:42.61 ATV.dial ARV @22:02:43.41 ASV.dial :PCRlock @22:02:43.80 delay 5 seconds @22:02:48.83 Running Rp Profile60C300 thermocycling @22:02:48.84 PCR.program Profile60C300 @22:02:48.98 Profile60C300 should complete in < 1:45:21 @22:02:48.99 PCR.run 2:16:41.25 @23:25:58.92 PCR.off @23:26:00.50 PCR for Rp completed -- closed /var/log/esp/Rp18sep1421h200ml.pcr @23:26:00.57 Cleaning PCR @23:26:00.58 AFV.select :carrierSolution @23:26:00.69 delay 2 seconds @23:26:02.72 AS.reconfigure ASconfig @23:26:02.77 AS.seek 0.5ml @23:26:13.11 delay 1 second @23:26:14.13 AFV.select :holdingCoil @23:26:14.23 ASV.dial :waste1 @23:26:14.61 ATV.dial :air3 @23:26:16.46 AS.reconfigure ASslow1 @23:26:16.51 AS.seek 0.51ml @23:26:18.80 delay 2 seconds @23:26:20.82 ATV.dial :bleach @23:26:21.45 AS.seek 0.81ml @23:27:22.13 delay 3 seconds @23:27:25.16 ATV.dial :air3 @23:27:25.69 delay 3 seconds @23:27:28.78 AS.seek 0.835ml @23:27:34.12 delay 3 seconds @23:27:37.15 ATV.dial :bleach @23:27:37.78 AS.seek 0.82ml @23:27:41.10 delay 3 seconds @23:27:44.13 ARV.dial :waste @23:27:44.75 delay 3 seconds @23:27:47.77 ATV.dial :ARV @23:27:48.75 AS.reconfigure ASconfig @23:27:48.81 AS.seek 0.47ml @23:28:09.22 AFV.select :carrierSolution @23:28:09.33 delay 2 seconds @23:28:11.37 AS.seek 1ml @23:28:42.13 delay 1 second @23:28:43.15 AFV.select :holdingCoil @23:28:43.25 delay 1 second @23:28:44.28 AS.reconfigure ASfast @23:28:44.33 AS.seek 0ml @23:29:11.67 AFV.select :carrierSolution @23:29:11.78 delay 2 seconds @23:29:13.81 AS.reconfigure ASconfig @23:29:13.86 AS.seek 1ml @23:30:11.63 delay 1 second @23:30:12.65 AFV.select :holdingCoil @23:30:12.75 delay 1 second @23:30:13.78 AS.reconfigure ASfast @23:30:13.83 AS.seek 0ml @23:30:41.14 AFV.select :carrierSolution @23:30:41.25 delay 2 seconds @23:30:43.28 AS.reconfigure ASconfig @23:30:43.33 AS.seek 1ml @23:31:41.08 delay 1 second @23:31:42.10 AFV.select :holdingCoil @23:31:42.20 delay 1 second @23:31:43.23 AS.reconfigure ASfast @23:31:43.28 AS.seek 0ml @23:32:10.62 ARV.dial between carrier and air1 @23:32:11.13 ATV.dial :air3 @23:32:12.40 AS.reconfigure ASslow1 @23:32:12.45 AS.seek 0.01ml @23:32:14.74 delay 2 seconds @23:32:16.76 ATV.dial :bleach @23:32:17.39 AS.seek 0.81ml @23:34:58.53 delay 3 seconds @23:35:01.56 ATV.dial :air3 @23:35:02.10 delay 3 seconds @23:35:05.19 AS.seek 0.835ml @23:35:10.50 delay 3 seconds @23:35:13.53 ATV.dial :bleach @23:35:14.14 AS.seek 0.82ml @23:35:17.44 delay 3 seconds @23:35:20.47 ATV.dial PCR @23:35:20.86 AS.reconfigure ASconfig @23:35:20.91 AS.seek :empty @23:36:08.38 PCR.program :bleachProfile @23:36:08.43 PCR program should complete in < 2:45 @23:36:08.44 PCR.run 10:00 @23:39:04.20 PCR.off @23:39:04.23 AFV.select :carrierSolution @23:39:04.34 delay 2 seconds @23:39:06.38 AS.seek 1ml @23:40:04.21 delay 1 second @23:40:05.23 AFV.select :holdingCoil @23:40:05.33 delay 1 second @23:40:06.36 AS.reconfigure ASfast @23:40:06.41 AS.seek 0ml @23:40:33.77 delay 3 seconds @23:40:36.80 AFV.select :carrierSolution @23:40:36.90 delay 2 seconds @23:40:38.93 AS.reconfigure ASconfig @23:40:38.99 AS.seek 1ml @23:41:36.75 delay 1 second @23:41:37.77 AFV.select :holdingCoil @23:41:37.87 delay 1 second @23:41:38.90 AS.reconfigure ASfast @23:41:38.95 AS.seek 0ml @23:42:06.30 delay 3 seconds @23:42:09.32 AFV.select :carrierSolution @23:42:09.42 delay 2 seconds @23:42:11.45 AS.reconfigure ASconfig @23:42:11.51 AS.seek 1ml @23:43:09.27 delay 1 second @23:43:10.28 AFV.select :holdingCoil @23:43:10.39 delay 1 second @23:43:11.42 AS.reconfigure ASfast @23:43:11.48 AS.seek 0ml @23:43:39.38 delay 3 seconds @23:43:42.40 AFV.select :carrierSolution @23:43:42.50 delay 2 seconds @23:43:44.53 AS.reconfigure ASconfig @23:43:44.59 AS.seek 1ml @23:44:42.35 delay 1 second @23:44:43.37 AFV.select :holdingCoil @23:44:43.47 delay 1 second @23:44:44.50 AS.reconfigure ASfast @23:44:44.56 AS.seek 0ml @23:45:11.93 delay 3 seconds @23:45:14.96 AFV.select :carrierSolution @23:45:15.06 delay 2 seconds @23:45:17.09 AS.reconfigure ASconfig @23:45:17.15 AS.seek 1ml @23:46:14.94 delay 1 second @23:46:15.96 AFV.select :holdingCoil @23:46:16.06 delay 1 second @23:46:17.09 AS.reconfigure ASnorm @23:46:17.14 AS.seek 0ml @23:47:07.87 Setting up PCR for Ss -- writing /var/log/esp/Ss18sep1423h200ml.pcr @23:47:07.89 Building PCR Slug from ASV with MM1 mix, PP2 primer @23:47:07.90 Priming with carrier @23:47:07.91 ATV.dial ARV @23:47:08.61 ARV.dial :carrier @23:47:08.96 AS.seek 0.5ml @23:47:34.52 AFV.select :carrierSolution @23:47:34.63 delay 2 seconds @23:47:36.66 AS.reconfigure ASconfig @23:47:36.71 AS.seek 1ml @23:48:05.74 delay 1 second @23:48:06.76 AFV.select :holdingCoil @23:48:06.86 delay 1 second @23:48:07.89 ATV.dial :waste @23:48:09.52 AS.reconfigure ASnorm @23:48:09.58 AS.seek :empty @23:49:00.35 AFV.select :carrierSolution @23:49:00.47 delay 2 seconds @23:49:02.50 AS.reconfigure ASconfig @23:49:02.55 AS.seek 0.5ml @23:49:31.64 delay 1 second @23:49:32.66 AFV.select :holdingCoil @23:49:32.76 Building Slug @23:49:32.77 ATV.dial :ARV @23:49:34.38 delay 2 seconds @23:49:36.41 ARV.dial :air1 @23:49:36.81 delay 2 seconds @23:49:38.90 AS.reconfigure ASslow2 @23:49:38.95 AS.seek 0.506ml @23:49:45.22 delay 2 seconds @23:49:47.25 ARV.dial :MM1 @23:49:47.86 delay 2 seconds @23:49:49.89 Adding MM1 @23:49:49.96 AS.seek 0.524ml @23:50:08.17 delay 25 seconds @23:50:33.20 ARV.dial :PP2 @23:50:33.58 Adding PP2 @23:50:33.60 delay 2 seconds @23:50:35.68 AS.seek 0.53ml @23:50:41.94 delay 10 seconds @23:50:51.97 ARV.dial :air2 @23:50:52.36 adding ARV air @23:50:52.38 delay 2 seconds @23:50:54.46 AS.seek 0.536ml @23:51:00.69 delay 5 seconds @23:51:05.72 ARV.dial :carrier @23:51:07.02 delay 7 seconds @23:51:14.11 AS.seek 0.594ml @23:52:12.61 delay 5 seconds @23:52:17.64 ATV.dial :ASV @23:52:18.07 delay 2 seconds @23:52:20.16 AS.seek 0.6ml @23:52:26.35 delay 4 seconds @23:52:30.38 ATV.dial ARV @23:52:31.03 delay 2 seconds @23:52:33.06 ARV.dial :carrier @23:52:33.07 delay 2 seconds @23:52:35.16 AS.seek 0.65ml @23:53:25.60 delay 4 seconds @23:53:29.63 ATV.dial PCR @23:53:30.24 ASV.dial :waste1 @23:53:30.25 ARV.dial :air1 @23:53:30.67 Positioning Slug by 0.324ml @23:53:30.76 AS.seek 0.326ml @23:58:56.77 delay 5 seconds @23:59:01.80 ATV.dial ARV @23:59:02.57 ASV.dial :PCRlock @23:59:02.96 delay 5 seconds @23:59:07.99 Running Ss Profile60C350 thermocycling @23:59:08.00 PCR.program Profile60C350 @23:59:08.14 Profile60C350 should complete in < 1:48:05 @23:59:08.16 PCR.run 2:20:06.25 @01:22:43.21CEST15-Sep-18 PCR.off @01:22:45.27 PCR for Ss completed -- closed /var/log/esp/Ss18sep1423h200ml.pcr @01:22:45.34 Cleaning PCR @01:22:45.35 AFV.select :carrierSolution @01:22:45.46 delay 2 seconds @01:22:47.49 AS.reconfigure ASconfig @01:22:47.54 AS.seek 0.5ml @01:22:58.39 delay 1 second @01:22:59.41 AFV.select :holdingCoil @01:22:59.51 ASV.dial :waste1 @01:22:59.89 ATV.dial :air3 @01:23:01.18 AS.reconfigure ASslow1 @01:23:01.23 AS.seek 0.51ml @01:23:03.50 delay 2 seconds @01:23:05.52 ATV.dial :bleach @01:23:06.14 AS.seek 0.81ml @01:24:06.85 delay 3 seconds @01:24:09.88 ATV.dial :air3 @01:24:10.43 delay 3 seconds @01:24:13.52 AS.seek 0.835ml @01:24:18.87 delay 3 seconds @01:24:21.90 ATV.dial :bleach @01:24:22.53 AS.seek 0.82ml @01:24:25.86 delay 3 seconds @01:24:28.89 ARV.dial :waste @01:24:29.50 delay 3 seconds @01:24:32.53 ATV.dial :ARV @01:24:33.42 AS.reconfigure ASconfig @01:24:33.47 AS.seek 0.47ml @01:24:53.85 AFV.select :carrierSolution @01:24:53.97 delay 2 seconds @01:24:56.01 AS.seek 1ml @01:25:26.80 delay 1 second @01:25:27.82 AFV.select :holdingCoil @01:25:27.92 delay 1 second @01:25:28.95 AS.reconfigure ASfast @01:25:29.00 AS.seek 0ml @01:25:56.35 AFV.select :carrierSolution @01:25:56.46 delay 2 seconds @01:25:58.49 AS.reconfigure ASconfig @01:25:58.54 AS.seek 1ml @01:26:56.28 delay 1 second @01:26:57.30 AFV.select :holdingCoil @01:26:57.40 delay 1 second @01:26:58.42 AS.reconfigure ASfast @01:26:58.47 AS.seek 0ml @01:27:25.82 AFV.select :carrierSolution @01:27:25.93 delay 2 seconds @01:27:27.96 AS.reconfigure ASconfig @01:27:28.01 AS.seek 1ml @01:28:25.75 delay 1 second @01:28:26.77 AFV.select :holdingCoil @01:28:26.87 delay 1 second @01:28:27.90 AS.reconfigure ASfast @01:28:27.95 AS.seek 0ml @01:28:55.34 ARV.dial between carrier and air1 @01:28:55.86 ATV.dial :air3 @01:28:57.15 AS.reconfigure ASslow1 @01:28:57.20 AS.seek 0.01ml @01:28:59.49 delay 2 seconds @01:29:01.51 ATV.dial :bleach @01:29:02.14 AS.seek 0.81ml @01:31:43.27 delay 3 seconds @01:31:46.30 ATV.dial :air3 @01:31:46.83 delay 3 seconds @01:31:49.92 AS.seek 0.835ml @01:31:55.19 delay 3 seconds @01:31:58.22 ATV.dial :bleach @01:31:58.82 AS.seek 0.82ml @01:32:02.11 delay 3 seconds @01:32:05.14 ATV.dial PCR @01:32:05.53 AS.reconfigure ASconfig @01:32:05.58 AS.seek :empty @01:32:53.07 PCR.program :bleachProfile @01:32:53.13 PCR program should complete in < 2:45 @01:32:53.14 PCR.run 10:00 @01:35:48.91 PCR.off @01:35:48.94 AFV.select :carrierSolution @01:35:49.05 delay 2 seconds @01:35:51.08 AS.seek 1ml @01:36:48.94 delay 1 second @01:36:49.96 AFV.select :holdingCoil @01:36:50.06 delay 1 second @01:36:51.09 AS.reconfigure ASfast @01:36:51.14 AS.seek 0ml @01:37:18.49 delay 3 seconds @01:37:21.52 AFV.select :carrierSolution @01:37:21.62 delay 2 seconds @01:37:23.65 AS.reconfigure ASconfig @01:37:23.71 AS.seek 1ml @01:38:21.46 delay 1 second @01:38:22.48 AFV.select :holdingCoil @01:38:22.58 delay 1 second @01:38:23.61 AS.reconfigure ASfast @01:38:23.66 AS.seek 0ml @01:38:51.02 delay 3 seconds @01:38:54.05 AFV.select :carrierSolution @01:38:54.15 delay 2 seconds @01:38:56.18 AS.reconfigure ASconfig @01:38:56.80 AS.seek 1ml @01:39:54.60 delay 1 second @01:39:55.62 AFV.select :holdingCoil @01:39:55.72 delay 1 second @01:39:56.75 AS.reconfigure ASfast @01:39:56.80 AS.seek 0ml @01:40:24.16 delay 3 seconds @01:40:27.19 AFV.select :carrierSolution @01:40:27.29 delay 2 seconds @01:40:29.32 AS.reconfigure ASconfig @01:40:29.38 AS.seek 1ml @01:41:27.14 delay 1 second @01:41:28.16 AFV.select :holdingCoil @01:41:28.26 delay 1 second @01:41:29.29 AS.reconfigure ASfast @01:41:29.34 AS.seek 0ml @01:41:56.72 delay 3 seconds @01:41:59.74 AFV.select :carrierSolution @01:41:59.85 delay 2 seconds @01:42:01.88 AS.reconfigure ASconfig @01:42:01.94 AS.seek 1ml @01:42:59.77 delay 1 second @01:43:00.79 AFV.select :holdingCoil @01:43:00.89 delay 1 second @01:43:01.92 AS.reconfigure ASnorm @01:43:01.97 AS.seek 0ml @01:43:52.73 Setting up PCR for Bb -- writing /var/log/esp/Bb18sep1501h200ml.pcr @01:43:52.75 Building PCR Slug from ASV with MM3 mix, PP5 primer @01:43:52.76 Priming with carrier @01:43:52.77 ATV.dial ARV @01:43:53.38 ARV.dial :carrier @01:43:53.76 AS.seek 0.5ml @01:44:19.33 AFV.select :carrierSolution @01:44:19.44 delay 2 seconds @01:44:21.47 AS.reconfigure ASconfig @01:44:21.52 AS.seek 1ml @01:44:50.53 delay 1 second @01:44:51.56 AFV.select :holdingCoil @01:44:51.66 delay 1 second @01:44:52.69 ATV.dial :waste @01:44:54.33 AS.reconfigure ASnorm @01:44:54.38 AS.seek :empty @01:45:45.13 AFV.select :carrierSolution @01:45:45.24 delay 2 seconds @01:45:47.27 AS.reconfigure ASconfig @01:45:47.32 AS.seek 0.5ml @01:46:16.39 delay 1 second @01:46:17.41 AFV.select :holdingCoil @01:46:17.51 Building Slug @01:46:17.52 ATV.dial :ARV @01:46:19.14 delay 2 seconds @01:46:21.17 ARV.dial :air1 @01:46:21.58 delay 2 seconds @01:46:23.66 AS.reconfigure ASslow2 @01:46:23.72 AS.seek 0.506ml @01:46:30.25 delay 2 seconds @01:46:32.27 ARV.dial :MM3 @01:46:33.33 delay 2 seconds @01:46:35.36 Adding MM3 @01:46:35.44 AS.seek 0.524ml @01:46:53.99 delay 25 seconds @01:47:19.02 ARV.dial :PP5 @01:47:19.41 Adding PP5 @01:47:19.42 delay 2 seconds @01:47:21.51 AS.seek 0.53ml @01:47:28.36 delay 10 seconds @01:47:38.39 ARV.dial :air3 @01:47:38.77 adding ARV air @01:47:38.78 delay 2 seconds @01:47:40.87 AS.seek 0.536ml @01:47:47.30 delay 5 seconds @01:47:52.33 ARV.dial :carrier @01:47:53.61 delay 7 seconds @01:48:00.70 AS.seek 0.594ml @01:48:59.32 delay 5 seconds @01:49:04.35 ATV.dial :ASV @01:49:04.74 delay 2 seconds @01:49:06.83 AS.seek 0.6ml @01:49:13.19 delay 4 seconds @01:49:17.22 ATV.dial ARV @01:49:17.75 delay 2 seconds @01:49:19.78 ARV.dial :carrier @01:49:19.79 delay 2 seconds @01:49:21.88 AS.seek 0.65ml @01:50:12.32 delay 4 seconds @01:50:16.35 ATV.dial PCR @01:50:16.96 ASV.dial :waste1 @01:50:16.97 ARV.dial :air1 @01:50:17.39 Positioning Slug by 0.324ml @01:50:17.48 AS.seek 0.326ml @01:55:43.47 delay 5 seconds @01:55:48.50 ATV.dial ARV @01:55:49.26 ASV.dial :PCRlock @01:55:49.66 delay 5 seconds @01:55:54.69 Running Bb Profile60C300 thermocycling @01:55:54.70 PCR.program Profile60C300 @01:55:54.84 Profile60C300 should complete in < 1:45:21 @01:55:54.85 PCR.run 2:16:41.25 @03:18:53.65 PCR.off @03:18:55.22 PCR for Bb completed -- closed /var/log/esp/Bb18sep1501h200ml.pcr @03:18:55.30 Cleaning PCR @03:18:55.31 AFV.select :carrierSolution @03:18:55.42 delay 2 seconds @03:18:57.45 AS.reconfigure ASconfig @03:18:57.50 AS.seek 0.5ml @03:19:07.80 delay 1 second @03:19:08.82 AFV.select :holdingCoil @03:19:08.92 ASV.dial :waste1 @03:19:09.30 ATV.dial :air3 @03:19:10.59 AS.reconfigure ASslow1 @03:19:10.64 AS.seek 0.51ml @03:19:12.94 delay 2 seconds @03:19:14.96 ATV.dial :bleach @03:19:16.18 AS.seek 0.81ml @03:20:16.77 delay 3 seconds @03:20:19.79 ATV.dial :air3 @03:20:20.33 delay 3 seconds @03:20:23.42 AS.seek 0.835ml @03:20:28.70 delay 3 seconds @03:20:31.73 ATV.dial :bleach @03:20:32.36 AS.seek 0.82ml @03:20:35.68 delay 3 seconds @03:20:38.71 ARV.dial :waste @03:20:39.33 delay 3 seconds @03:20:42.36 ATV.dial :ARV @03:20:43.33 AS.reconfigure ASconfig @03:20:43.39 AS.seek 0.47ml @03:21:03.80 AFV.select :carrierSolution @03:21:03.91 delay 2 seconds @03:21:05.95 AS.seek 1ml @03:21:36.71 delay 1 second @03:21:37.74 AFV.select :holdingCoil @03:21:37.84 delay 1 second @03:21:38.87 AS.reconfigure ASfast @03:21:38.93 AS.seek 0ml @03:22:06.28 AFV.select :carrierSolution @03:22:06.39 delay 2 seconds @03:22:08.42 AS.reconfigure ASconfig @03:22:08.47 AS.seek 1ml @03:23:06.22 delay 1 second @03:23:07.25 AFV.select :holdingCoil @03:23:07.35 delay 1 second @03:23:08.38 AS.reconfigure ASfast @03:23:08.43 AS.seek 0ml @03:23:35.82 AFV.select :carrierSolution @03:23:35.93 delay 2 seconds @03:23:37.96 AS.reconfigure ASconfig @03:23:38.01 AS.seek 1ml @03:24:35.77 delay 1 second @03:24:36.80 AFV.select :holdingCoil @03:24:36.90 delay 1 second @03:24:37.93 AS.reconfigure ASfast @03:24:37.98 AS.seek 0ml @03:25:05.33 ARV.dial between carrier and air1 @03:25:05.83 ATV.dial :air3 @03:25:07.11 AS.reconfigure ASslow1 @03:25:07.16 AS.seek 0.01ml @03:25:09.53 delay 2 seconds @03:25:11.55 ATV.dial :bleach @03:25:12.17 AS.seek 0.81ml @03:27:53.38 delay 3 seconds @03:27:56.41 ATV.dial :air3 @03:27:56.94 delay 3 seconds @03:28:00.03 AS.seek 0.835ml @03:28:05.33 delay 3 seconds @03:28:08.36 ATV.dial :bleach @03:28:08.98 AS.seek 0.82ml @03:28:12.28 delay 3 seconds @03:28:15.31 ATV.dial PCR @03:28:15.71 AS.reconfigure ASconfig @03:28:15.76 AS.seek :empty @03:29:03.24 PCR.program :bleachProfile @03:29:03.29 PCR program should complete in < 2:45 @03:29:03.31 PCR.run 10:00 @03:31:59.07 PCR.off @03:31:59.09 AFV.select :carrierSolution @03:31:59.20 delay 2 seconds @03:32:01.24 AS.seek 1ml @03:32:59.01 delay 1 second @03:33:00.04 AFV.select :holdingCoil @03:33:00.14 delay 1 second @03:33:01.17 AS.reconfigure ASfast @03:33:01.22 AS.seek 0ml @03:33:28.57 delay 3 seconds @03:33:31.59 AFV.select :carrierSolution @03:33:31.69 delay 2 seconds @03:33:33.72 AS.reconfigure ASconfig @03:33:33.79 AS.seek 1ml @03:34:31.60 delay 1 second @03:34:32.62 AFV.select :holdingCoil @03:34:32.72 delay 1 second @03:34:33.75 AS.reconfigure ASfast @03:34:33.80 AS.seek 0ml @03:35:01.16 delay 3 seconds @03:35:04.19 AFV.select :carrierSolution @03:35:04.29 delay 2 seconds @03:35:06.32 AS.reconfigure ASconfig @03:35:06.38 AS.seek 1ml @03:36:04.20 delay 1 second @03:36:05.23 AFV.select :holdingCoil @03:36:05.33 delay 1 second @03:36:06.36 AS.reconfigure ASfast @03:36:06.41 AS.seek 0ml @03:36:33.77 delay 3 seconds @03:36:36.80 AFV.select :carrierSolution @03:36:36.90 delay 2 seconds @03:36:38.93 AS.reconfigure ASconfig @03:36:39.00 AS.seek 1ml @03:37:36.75 delay 1 second @03:37:37.76 AFV.select :holdingCoil @03:37:37.87 delay 1 second @03:37:38.90 AS.reconfigure ASfast @03:37:39.52 AS.seek 0ml @03:38:06.88 delay 3 seconds @03:38:09.90 AFV.select :carrierSolution @03:38:10.01 delay 2 seconds @03:38:12.04 AS.reconfigure ASconfig @03:38:12.10 AS.seek 1ml @03:39:09.88 delay 1 second @03:39:10.91 AFV.select :holdingCoil @03:39:11.01 delay 1 second @03:39:12.04 AS.reconfigure ASnorm @03:39:12.10 AS.seek 0ml @03:40:02.82 Setting up PCR for IPc -- writing /var/log/esp/IPc18sep1503h200ml.pcr @03:40:02.84 Building PCR Slug from ASV with MM1 mix, PP1 primer @03:40:02.85 Priming with carrier @03:40:02.86 ATV.dial ARV @03:40:03.47 ARV.dial :carrier @03:40:03.81 AS.seek 0.5ml @03:40:29.39 AFV.select :carrierSolution @03:40:29.50 delay 2 seconds @03:40:31.53 AS.reconfigure ASconfig @03:40:31.58 AS.seek 1ml @03:41:00.60 delay 1 second @03:41:01.62 AFV.select :holdingCoil @03:41:01.72 delay 1 second @03:41:02.75 ATV.dial :waste @03:41:04.39 AS.reconfigure ASnorm @03:41:04.45 AS.seek :empty @03:41:55.18 AFV.select :carrierSolution @03:41:55.29 delay 2 seconds @03:41:57.32 AS.reconfigure ASconfig @03:41:57.38 AS.seek 0.5ml @03:42:26.50 delay 1 second @03:42:27.52 AFV.select :holdingCoil @03:42:27.62 Building Slug @03:42:27.63 ATV.dial :ARV @03:42:29.24 delay 2 seconds @03:42:31.27 ARV.dial :air1 @03:42:31.66 delay 2 seconds @03:42:33.74 AS.reconfigure ASslow2 @03:42:33.81 AS.seek 0.506ml @03:42:39.99 delay 2 seconds @03:42:42.02 ARV.dial :MM1 @03:42:42.63 delay 2 seconds @03:42:44.66 Adding MM1 @03:42:44.73 AS.seek 0.524ml @03:43:03.08 delay 25 seconds @03:43:28.11 ARV.dial :PP1 @03:43:28.50 Adding PP1 @03:43:28.52 delay 2 seconds @03:43:30.60 AS.seek 0.53ml @03:43:37.02 delay 10 seconds @03:43:47.05 ARV.dial :air1 @03:43:47.44 adding ARV air @03:43:47.45 delay 2 seconds @03:43:49.54 AS.seek 0.536ml @03:43:55.74 delay 5 seconds @03:44:00.77 ARV.dial :carrier @03:44:01.18 delay 7 seconds @03:44:08.27 AS.seek 0.594ml @03:45:06.85 delay 5 seconds @03:45:11.88 ATV.dial :ASV @03:45:12.27 delay 2 seconds @03:45:14.36 AS.seek 0.6ml @03:45:20.66 delay 4 seconds @03:45:24.69 ATV.dial ARV @03:45:25.22 delay 2 seconds @03:45:27.25 ARV.dial :carrier @03:45:27.26 delay 2 seconds @03:45:29.35 AS.seek 0.65ml @03:46:20.07 delay 4 seconds @03:46:24.10 ATV.dial PCR @03:46:24.71 ASV.dial :waste1 @03:46:24.72 ARV.dial :air1 @03:46:25.14 Positioning Slug by 0.324ml @03:46:25.23 AS.seek 0.326ml @03:51:51.54 delay 5 seconds @03:51:56.57 ATV.dial ARV @03:51:57.33 ASV.dial :PCRlock @03:51:57.72 delay 5 seconds @03:52:02.75 Running IPc ProfileIPc thermocycling @03:52:02.76 PCR.program ProfileIPc @03:52:02.91 ProfileIPc should complete in < 1:50:49 @03:52:02.92 PCR.run 2:23:31.25 @05:17:16.02 PCR.off @05:17:19.14 PCR for IPc completed -- closed /var/log/esp/IPc18sep1503h200ml.pcr @05:17:19.15 Full MFB Decontamination @05:17:19.17 Decon extraction column and decon AIV @05:17:19.19 SPE.hold 2:46:40,55C @05:17:19.23 ASV.dial :SPEdelivery @05:17:19.89 delay 20 seconds @05:17:39.92 AFV.select :carrierSolution @05:17:40.02 delay 2 seconds @05:17:42.05 AS.reconfigure ASconfig @05:17:42.11 AS.seek 0.5ml @05:17:52.41 delay 1 second @05:17:53.43 AFV.select :holdingCoil @05:17:53.53 ATV.dial :air1 @05:17:55.53 AS.reconfigure ASslow1 @05:17:55.58 AS.seek 0.52ml @05:17:59.87 ATV.dial :ASV @05:18:01.67 ASV.dial :SPErecovery @05:18:02.39 AS.seek 0.62ml @05:18:22.80 ATV.dial :waste @05:18:24.83 AS.reconfigure ASnorm @05:18:24.88 AS.seek :empty @05:18:56.52 AFV.select :holdingCoil @05:18:56.53 ATV.dial :waste @05:18:56.55 AS.reconfigure ASconfig @05:18:56.60 AS.seek 0ml @05:18:56.88 AFV.select :carrierSolution @05:18:57.00 delay 2 seconds @05:18:59.04 AS.seek 0.5ml @05:19:28.07 delay 1 second @05:19:29.08 AFV.select :holdingCoil @05:19:29.19 ATV.dial :air3 @05:19:29.91 AS.reconfigure ASslow1 @05:19:29.97 AS.seek 0.52ml @05:19:34.30 ATV.dial :air3 @05:19:34.39 AS.seek 0.53ml @05:19:36.80 delay 2 seconds @05:19:38.82 ATV.dial :bleach @05:19:39.32 AS.seek 0.71ml @05:20:15.82 delay 3 seconds @05:20:18.85 ATV.dial :air3 @05:20:19.44 delay 3 seconds @05:20:22.53 AS.seek 0.735ml @05:20:27.86 delay 3 seconds @05:20:30.88 ATV.dial :bleach @05:20:31.54 AS.seek 0.72ml @05:20:34.88 delay 3 seconds @05:20:37.91 ATV.dial ASV @05:20:38.99 ASV.dial :SPErecovery @05:20:39.08 AS.seek 0.365ml @05:21:50.77 ATV.dial :waste @05:21:52.52 AS.reconfigure ASnorm @05:21:52.57 AS.seek :empty @05:22:11.35 ATV.dial :air3 @05:22:12.06 AS.reconfigure ASslow1 @05:22:12.11 AS.seek 0.009ml @05:22:14.42 delay 2 seconds @05:22:16.45 ATV.dial :bleach @05:22:16.93 AS.seek 0.579ml @05:24:11.83 delay 3 seconds @05:24:14.85 ATV.dial :air3 @05:24:15.43 delay 3 seconds @05:24:18.51 AS.seek 0.604ml @05:24:23.81 delay 3 seconds @05:24:26.84 ATV.dial :bleach @05:24:27.46 AS.seek 0.589ml @05:24:30.77 delay 3 seconds @05:24:33.80 ATV.dial :ASV @05:24:34.82 ASV.dial :wasteR @05:24:35.23 AS.seek 0ml @05:26:33.99 delay 1:00 @05:27:34.02 ATV.dial ASV @05:27:34.03 AFV.select :carrierSolution @05:27:34.13 delay 2 seconds @05:27:36.16 AS.reconfigure ASconfig @05:27:36.21 AS.seek 1ml @05:28:33.97 delay 1 second @05:28:34.99 AFV.select :holdingCoil @05:28:35.09 delay 1 second @05:28:36.12 ASV.dial :wasteR @05:28:36.14 AS.reconfigure ASslow1 @05:28:36.75 AS.seek 0ml @05:31:58.16 delay 10 seconds @05:32:08.19 ATV.dial :air3 @05:32:09.68 AS.seek 0.01ml @05:32:12.10 delay 2 seconds @05:32:14.13 ATV.dial :bleach @05:32:14.76 AS.seek 0.58ml @05:34:09.69 delay 3 seconds @05:34:12.72 ATV.dial :air3 @05:34:13.27 delay 3 seconds @05:34:16.36 AS.seek 0.605ml @05:34:21.67 delay 3 seconds @05:34:24.70 ATV.dial :bleach @05:34:25.32 AS.seek 0.59ml @05:34:28.64 delay 3 seconds @05:34:31.67 ATV.dial ASV @05:34:32.80 ASV.dial :SPEdelivery @05:34:33.19 AS.seek 0ml @05:36:32.10 delay 1:00 @05:37:32.12 AFV.select :carrierSolution @05:37:32.23 delay 2 seconds @05:37:34.26 AS.reconfigure ASconfig @05:37:34.31 AS.seek 1ml @05:38:32.08 delay 1 second @05:38:33.10 AFV.select :holdingCoil @05:38:33.20 delay 1 second @05:38:34.23 ATV.dial ASV @05:38:34.24 ASV.dial :SPEdelivery @05:38:34.26 AS.reconfigure ASslow1 @05:38:34.31 AS.seek 0ml @05:41:55.71 delay 10 seconds @05:42:05.74 ATV.dial :air2 @05:42:06.56 AS.reconfigure ASslow0 @05:42:06.61 AS.seek 0.8ml @05:43:27.39 delay 3 seconds @05:43:30.42 ATV.dial :ASV @05:43:31.39 ASV.dial :SPEdelivery @05:43:31.41 AS.reconfigure ASslow1 @05:43:31.46 AS.seek 0.03ml @05:46:06.61 delay 20 seconds @05:46:26.64 ATV.dial :waste,via: :air3 @05:46:28.35 AS.reconfigure ASconfig @05:46:28.40 AS.seek :empty @05:46:30.47 SPE.coast @05:46:30.56 Removing Bleach from Mixing Coil @05:46:30.57 AFV.select :carrierSolution @05:46:30.67 delay 2 seconds @05:46:32.71 AS.seek 0.5ml @05:47:01.80 delay 1 second @05:47:02.82 AFV.select :holdingCoil @05:47:02.92 ATV.dial :ASV @05:47:04.58 ASV.dial :SPErecovery @05:47:05.23 AS.reconfigure ASslow1 @05:47:05.28 AS.seek 0.73ml @05:47:51.86 ASV.dial :wasteD @05:47:52.43 AS.reconfigure ASnorm @05:47:52.49 AS.seek 0ml @05:48:29.58 AFV.select :carrierSolution @05:48:29.69 delay 2 seconds @05:48:31.72 AS.reconfigure ASconfig @05:48:31.77 AS.seek 0.5ml @05:49:00.83 delay 1 second @05:49:01.85 AFV.select :holdingCoil @05:49:01.95 ATV.dial ASV @05:49:01.96 ASV.dial :SPErecovery @05:49:02.62 AS.reconfigure ASslow0 @05:49:02.67 AS.seek 0.3ml @05:49:23.11 ATV.dial :air2 @05:49:24.14 AS.reconfigure ASslow1 @05:49:24.19 AS.seek 0.32ml @05:49:28.54 ATV.dial ASV @05:49:29.57 AS.reconfigure ASslow0 @05:49:29.62 AS.seek 0.57ml @05:49:55.07 ASV.dial :wasteD @05:49:55.67 AS.reconfigure ASnorm @05:49:55.72 AS.seek 0ml @05:50:24.80 AFV.select :carrierSolution @05:50:24.91 delay 2 seconds @05:50:26.94 AS.reconfigure ASconfig @05:50:26.99 AS.seek 0.5ml @05:50:56.02 delay 1 second @05:50:57.04 AFV.select :holdingCoil @05:50:57.14 ATV.dial ASV @05:50:57.15 ASV.dial :SPErecovery @05:50:57.81 AS.reconfigure ASslow0 @05:50:57.86 AS.seek 0.3ml @05:51:18.32 ATV.dial :air2 @05:51:19.36 AS.reconfigure ASslow1 @05:51:19.41 AS.seek 0.32ml @05:51:23.76 ATV.dial ASV @05:51:25.37 AS.reconfigure ASslow0 @05:51:25.42 AS.seek 0.57ml @05:51:50.83 ASV.dial :wasteD @05:51:51.53 AS.reconfigure ASnorm @05:51:51.58 AS.seek 0ml @05:52:20.66 ATV.dial :air2 @05:52:21.63 AS.reconfigure ASslow0 @05:52:21.69 AS.seek 0.3ml @05:52:52.21 ATV.dial :ASV @05:52:53.18 ASV.dial :wasteD @05:52:53.20 AS.reconfigure ASconfig @05:52:53.25 AS.seek 0.05ml @05:53:07.96 ATV.dial :waste @05:53:09.80 AS.seek 0ml @05:53:13.03 ASV.dial :SPEdelivery @05:53:14.08 deconLysateINT @05:53:14.09 ATV.dial :air3 @05:53:14.76 AS.reconfigure ASslow1 @05:53:14.81 AS.seek 0.01ml @05:53:17.21 delay 2 seconds @05:53:19.24 ATV.dial :bleach @05:53:19.68 AS.seek 0.96ml @05:56:31.02 delay 3 seconds @05:56:34.05 ATV.dial :air3 @05:56:34.58 delay 3 seconds @05:56:37.67 AS.seek 0.985ml @05:56:42.98 delay 3 seconds @05:56:46.01 ATV.dial :bleach @05:56:46.62 AS.seek 0.97ml @05:56:49.91 delay 3 seconds @05:56:52.93 ATV.dial AIV @05:56:54.16 AIV.dial :coilToMFB @05:56:54.18 AWV.select :waste @05:56:54.19 AFV.select :carrierSolution @05:56:54.30 delay 2 seconds @05:56:56.33 AS.reconfigure ASconfig @05:56:56.38 AS.seek 1ml @05:56:58.41 delay 1 second @05:56:59.43 AFV.select :holdingCoil @05:56:59.53 delay 1 second @05:57:00.56 AS.reconfigure ASslow0 @05:57:00.61 AS.seek :empty @05:58:41.55 AFV.select :carrierSolution @05:58:41.66 delay 2 seconds @05:58:43.69 AS.reconfigure ASconfig @05:58:43.74 AS.seek 1ml @05:59:41.58 delay 1 second @05:59:42.60 AFV.select :holdingCoil @05:59:42.70 delay 1 second @05:59:43.73 AS.reconfigure ASslow0 @05:59:43.78 AS.seek :empty @06:01:24.70 AFV.select :carrierSolution @06:01:24.82 delay 2 seconds @06:01:26.85 AS.reconfigure ASconfig @06:01:26.90 AS.seek 1ml @06:02:24.74 delay 1 second @06:02:25.76 AFV.select :holdingCoil @06:02:25.87 delay 1 second @06:02:26.90 AS.reconfigure ASslow0 @06:02:26.95 AS.seek :empty @06:04:07.88 ATV.dial :air2 @06:04:08.54 AS.reconfigure ASslow1 @06:04:08.59 AS.seek 0.009ml @06:04:10.94 AS.reconfigure ASslow0 @06:04:11.00 AS.seek 1ml @06:05:50.94 ATV.dial AIV @06:05:51.70 AS.seek 0.02ml @06:07:30.60 ATV.dial :waste @06:07:32.23 AS.seek 0ml @06:07:34.53 AWV.select :air @06:07:34.71 Cleaning PCR @06:07:34.72 AFV.select :carrierSolution @06:07:34.83 delay 2 seconds @06:07:36.86 AS.reconfigure ASconfig @06:07:36.91 AS.seek 0.5ml @06:08:05.94 delay 1 second @06:08:06.96 AFV.select :holdingCoil @06:08:07.06 ASV.dial :waste1 @06:08:07.44 ATV.dial :air3 @06:08:08.11 AS.reconfigure ASslow1 @06:08:08.16 AS.seek 0.51ml @06:08:10.43 delay 2 seconds @06:08:12.45 ATV.dial :bleach @06:08:12.93 AS.seek 0.81ml @06:09:13.55 delay 3 seconds @06:09:16.57 ATV.dial :air3 @06:09:17.11 delay 3 seconds @06:09:20.19 AS.seek 0.835ml @06:09:25.55 delay 3 seconds @06:09:28.58 ATV.dial :bleach @06:09:29.18 AS.seek 0.82ml @06:09:33.07 delay 3 seconds @06:09:36.10 ARV.dial :waste @06:09:36.72 delay 3 seconds @06:09:39.75 ATV.dial :ARV @06:09:40.62 AS.reconfigure ASconfig @06:09:40.67 AS.seek 0.47ml @06:10:01.08 AFV.select :carrierSolution @06:10:01.19 delay 2 seconds @06:10:03.23 AS.seek 1ml @06:10:34.00 delay 1 second @06:10:35.02 AFV.select :holdingCoil @06:10:35.12 delay 1 second @06:10:36.15 AS.reconfigure ASfast @06:10:36.20 AS.seek 0ml @06:11:03.54 AFV.select :carrierSolution @06:11:03.65 delay 2 seconds @06:11:05.68 AS.reconfigure ASconfig @06:11:05.73 AS.seek 1ml @06:12:03.52 delay 1 second @06:12:04.55 AFV.select :holdingCoil @06:12:04.65 delay 1 second @06:12:05.68 AS.reconfigure ASfast @06:12:05.73 AS.seek 0ml @06:12:33.07 AFV.select :carrierSolution @06:12:33.18 delay 2 seconds @06:12:35.21 AS.reconfigure ASconfig @06:12:35.26 AS.seek 1ml @06:13:33.08 delay 1 second @06:13:34.10 AFV.select :holdingCoil @06:13:34.20 delay 1 second @06:13:35.23 AS.reconfigure ASfast @06:13:35.28 AS.seek 0ml @06:14:02.71 ARV.dial between carrier and air1 @06:14:03.21 ATV.dial :air3 @06:14:04.48 AS.reconfigure ASslow1 @06:14:04.53 AS.seek 0.01ml @06:14:06.88 delay 2 seconds @06:14:08.91 ATV.dial :bleach @06:14:09.53 AS.seek 0.81ml @06:16:50.69 delay 3 seconds @06:16:53.72 ATV.dial :air3 @06:16:54.27 delay 3 seconds @06:16:57.44 AS.seek 0.835ml @06:17:02.88 delay 3 seconds @06:17:05.91 ATV.dial :bleach @06:17:06.53 AS.seek 0.82ml @06:17:09.89 delay 3 seconds @06:17:12.92 ATV.dial PCR @06:17:13.32 AS.reconfigure ASconfig @06:17:13.37 AS.seek :empty @06:18:00.85 PCR.program :bleachProfile @06:18:00.91 PCR program should complete in < 2:45 @06:18:00.92 PCR.run 10:00 @06:21:02.74 PCR.off @06:21:02.77 AFV.select :carrierSolution @06:21:02.88 delay 2 seconds @06:21:04.92 AS.seek 1ml @06:22:02.75 delay 1 second @06:22:03.77 AFV.select :holdingCoil @06:22:03.87 delay 1 second @06:22:04.90 AS.reconfigure ASfast @06:22:04.95 AS.seek 0ml @06:22:32.29 delay 3 seconds @06:22:35.32 AFV.select :carrierSolution @06:22:35.42 delay 2 seconds @06:22:37.45 AS.reconfigure ASconfig @06:22:37.51 AS.seek 1ml @06:23:35.33 delay 1 second @06:23:36.35 AFV.select :holdingCoil @06:23:36.45 delay 1 second @06:23:37.48 AS.reconfigure ASfast @06:23:37.53 AS.seek 0ml @06:24:04.88 delay 3 seconds @06:24:07.91 AFV.select :carrierSolution @06:24:08.01 delay 2 seconds @06:24:10.04 AS.reconfigure ASconfig @06:24:10.11 AS.seek 1ml @06:25:07.91 delay 1 second @06:25:08.93 AFV.select :holdingCoil @06:25:09.03 delay 1 second @06:25:10.06 AS.reconfigure ASfast @06:25:10.12 AS.seek 0ml @06:25:37.58 delay 3 seconds @06:25:40.61 AFV.select :carrierSolution @06:25:40.71 delay 2 seconds @06:25:42.74 AS.reconfigure ASconfig @06:25:42.80 AS.seek 1ml @06:26:40.60 delay 1 second @06:26:41.62 AFV.select :holdingCoil @06:26:41.72 delay 1 second @06:26:42.75 AS.reconfigure ASfast @06:26:42.80 AS.seek 0ml @06:27:10.16 delay 3 seconds @06:27:13.18 AFV.select :carrierSolution @06:27:13.28 delay 2 seconds @06:27:15.31 AS.reconfigure ASconfig @06:27:15.36 AS.seek 1ml @06:28:13.16 delay 1 second @06:28:14.17 AFV.select :holdingCoil @06:28:14.28 delay 1 second @06:28:15.80 AS.reconfigure ASnorm @06:28:15.92 AS.seek 0ml @06:29:06.67 AFV.select :carrierSolution @06:29:06.78 delay 2 seconds @06:29:08.81 AS.reconfigure ASconfig @06:29:08.86 AS.seek 1ml @06:30:06.63 delay 1 second @06:30:07.65 AFV.select :holdingCoil @06:30:07.75 delay 1 second @06:30:08.77 ATV.dial :waste @06:30:09.98 AS.reconfigure ASnorm @06:30:10.03 AS.seek 0.5ml @06:30:35.60 ATV.dial :waste2 @06:30:36.87 AS.seek 0ml @06:31:02.42
Asleep after completing 18tun1 assays @06:31:02.44 GoodNews.email "{1 => 9, 2 => 13, 3 => 22, 4 => 22, 5 => 22, 6 => 4, 7 => 6}\nCan@06:25:28, 21.5C, 51% humidity, 14.6psia, 12.001V, 0.727A, 0.727A avg, 8.72W",Subject: "Asleep after completing 18tun1 assays" @06:31:02.46
Mission runtime was 12:25:34.0038 @21:35:42.75CEST15-Sep-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @21:35:45.71 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @21:35:46.29 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @21:35:53.30 Polling Can every 10:00 @21:35:55.13 SPE.coast @21:35:55.20 SPE.configure SPEconfig @21:35:55.30 AS.configure ASconfig @21:35:55.40 SS.reconfigure SSconfig @21:35:55.45 SS.hold @21:35:55.50 SS.configure SSconfig @21:35:55.60 CC.reconfigure CCconfig @21:35:55.66 CC.hold @21:35:55.73 CC.configure CCconfig @21:35:55.83 CS.reconfigure CSconfig @21:35:55.87 CS.hold @21:35:55.95 CS.configure CSconfig @21:35:56.05 PC.reconfigure PCconfig @21:35:56.10 PC.hold @21:35:56.52 PC.configure PCconfig @21:35:56.62 PS.configure PSconfig @21:35:56.73 Forearm.reconfigure LooseForearm @21:35:56.78 Forearm.hold @21:35:56.83 Forearm.configure LooseForearm @21:35:56.93 Elbow.reconfigure LooseElbow @21:35:56.97 Elbow.hold @21:35:57.04 Elbow.configure LooseElbow @21:35:57.16 SC.reconfigure SCloose @21:35:57.21 SC.hold @21:35:57.26 SC.configure SCloose @21:35:57.35 SE.reconfigure SEconfig @21:35:57.40 SE.hold @21:35:57.46 SE.configure SEconfig @21:35:57.51 CH.coast @21:35:57.56 CH.configure CHconfig @21:35:57.59 PH.coast @21:35:57.65 PH.configure PHconfig @21:35:57.69 PCR.off @21:35:57.72 PCR.pidtemp= 50,0.1,0,300,25,25 @21:35:57.75 PCR.caltemp= 2.7073,0.0373,0 @21:35:57.78 All dwarf controllers running firmware version 3.75 @21:35:57.89 Cleanly exited at 21:19:43CEST15-Sep-18 @21:35:57.93 Loading /home/esp/esp2/mission/18tun1.rb mission @21:35:58.00 Preparing to sleep until +0:00:15 @21:35:58.03 GoodNews.email "Starting 18tun1 w/pucks in tube 2\n{3 => 22, 4 => 22, 5 => 22}\nCan@21:35:53, 19.4C, 32% humidity, 14.7psia, 11.987V, 0.733A, 0.727A avg, 8.79W",Subject: "18tun1 begun" @21:35:58.11 SS.coast @21:35:58.14
Asleep til +0:00:15 @21:35:58.18 Continuing at 21:36 @21:35:58.20 CC.coast @21:35:58.29 CS.coast @21:35:58.36 PC.coast @21:35:58.40 Forearm.coast @21:35:58.43 Elbow.coast @21:35:58.47 SC.coast @21:35:58.51 SE.coast @21:35:58.90 Gate.power :core,:OFF @21:35:58.91 Gate.power :raw,:OFF @21:35:58.92 Shutdown @21:36:06.79 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:105\n{3 => 22, 4 => 22, 5 => 22}\nCan@21:35:58, 19.3C, 32% humidity, 14.7psia, 11.992V, 0.720A, 0.720A avg, 8.64W",Subject: "Asleep until 21:36" @21:36:12.96
Powering core bus @21:36:17.97 Gate.power :raw,:ON @21:36:18.63 SPE.coast @21:36:18.72 SPE.configure SPEconfig @21:36:18.81 AS.configure ASconfig @21:36:18.91 SS.configure SSconfig @21:36:19.05 CC.configure CCconfig @21:36:19.19 CS.configure CSconfig @21:36:19.29 PC.configure PCconfig @21:36:19.38 PS.configure PSconfig @21:36:19.50 Forearm.configure LooseForearm @21:36:19.60 Elbow.configure LooseElbow @21:36:19.72 SC.configure SCloose @21:36:19.82 SE.configure SEconfig @21:36:19.87 CH.coast @21:36:19.92 CH.configure CHconfig @21:36:19.96 PH.coast @21:36:20.02 PH.configure PHconfig @21:36:20.06 PCR.off @21:36:20.09 PCR.pidtemp= 50,0.1,0,300,25,25 @21:36:20.11 PCR.caltemp= 2.7073,0.0373,0 @21:36:20.14 All dwarf controllers running firmware version 3.75 @21:36:20.26 Valve.reset @21:36:20.43 PCR.off @21:36:20.46 PCR.pidtemp= 50,0.1,0,300,25,25 @21:36:20.49 PCR.caltemp= 2.7073,0.0373,0 @21:36:20.67 Intake.select :close @21:36:20.70 Exhaust.select :close @21:36:20.77 CTV.dial :air @21:36:20.86 PSV.dial :air @21:36:20.89 CSV.dial :waste1 @21:36:21.07 PS.reconfigure PSconfig @21:36:21.10
Forearm.reconfigure LooseForearm @21:36:21.21 PS.home.seek 0ml @21:36:21.22
Forearm.home.seek :retract @21:36:21.33 SSV.dial :spit,avoiding: :exhaust @21:36:21.47 CS.reconfigure CSfast @21:36:21.51 SS.reconfigure SSconfig @21:36:21.59 CS.home.seek 0ml @21:36:21.64 SS.home.seek 0ml @21:36:22.02 Hand.close @21:36:22.05
Elbow.jog 1500 @21:36:22.80 Elbow.reconfigure LooseElbow @21:36:22.86 Elbow.home.seek :clear @21:36:26.26 SE.reconfigure SEconfig @21:36:26.31 SE.home.seek :down @21:36:27.00 SC.reconfigure SCsloppy @21:36:27.05 SC.home.seek :initial @21:36:30.68 PS.coast @21:36:31.07
Forearm.seek :retract @21:36:31.49 Elbow.seek :hammer @21:36:32.70 Forearm.reconfigure TightForearm @21:36:32.75 Forearm.seek 5 + 350 counts @21:36:33.87 Forearm.seek :retract @21:36:35.08 PC.reconfigure PCconfig @21:36:35.10
CC.reconfigure CCconfig @21:36:35.19 PC.home.seek :open @21:36:35.26
CC.home.seek :open @21:36:38.56 Phase [mbaPhase] @21:36:38.57CEST15-Sep-18 ==================> STARTED MBAsuperphase (phase #1) <=== @21:36:38.66 Starting mfb @21:36:38.67 pcrBAC sampling at most 200ml, wcr at most 200ml @21:36:38.76 Loading sh1 puck from tube 2 into Collection Clamp @21:36:38.85 Hand.open @21:36:38.88 Readying the MFB @21:36:38.92 Homing MFB Syringe @21:36:38.94 AFV.select :holdingCoil @21:36:39.10 ATV.dial :air2 @21:36:39.86
SC.reconfigure SCloose @21:36:40.04 Elbow.reconfigure TightElbow @21:36:40.09 Elbow.seek 2 @21:36:40.53 Forearm.seek 2 + 700 counts @21:36:40.61 AS.reconfigure ASconfig @21:36:40.66 AS.home.seek :home @21:36:41.72 Session Begun @21:36:41.84
SE.reconfigure SEpick @21:36:41.96 SE.seek beamBreak @21:36:43.00 SE.configure SEconfig @21:36:43.21 Elbow.seek 2 @21:36:43.44 Forearm.seek 2 + 300 counts @21:36:43.74 Forearm.reconfigure LooseForearm @21:36:43.81 Hand.close @21:36:44.63 SE.seek :down @21:36:44.88
Forearm.seek :retract @21:36:45.54 SE.coast @21:36:45.86
Elbow.seek CC @21:36:46.17 AFV.select :carrierSolution @21:36:46.31 delay 2 seconds @21:36:47.56
Elbow.reconfigure LooseElbow @21:36:47.69 Forearm.reconfigure TightForearm @21:36:47.77 Forearm.seek CC @21:36:48.34 AS.seek 1ml @21:36:49.08
CC.seek :closed @21:36:53.45 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.57 @21:36:53.46 Hand.open @21:36:54.31 Forearm.reconfigure LooseForearm @21:36:54.38 Forearm.seek :retract @21:36:55.40 SC.coast @21:36:55.47 collectSample 200ml w/bubble point of 55psi @21:36:55.49 Priming 7ml @21:36:55.51 Intake.select :close @21:36:55.53 Exhaust.select :close @21:36:55.57 SS.hold @21:36:55.87 Calibrating pressure and temperature sensors @21:36:55.89 CTV.dial :air @21:36:55.96 SSV.dial :spit,avoiding: :exhaust @21:36:56.01 SS.reconfigure SSfast @21:36:56.10 SS.seek 5ml @21:36:59.14 SSV.dial CBV @21:36:59.17
CBV.dial SSV @21:37:00.71 delay 3.8 seconds @21:37:04.65 SSV.dial :spit @21:37:06.64 SS.seek 0ml @21:37:09.42 SSV.dial CBV @21:37:10.95 CTV.dial :intake @21:37:11.85 SSV.dial CBV @21:37:11.88
CBV.dial SSV @21:37:11.91 Allowing 40s for 7.0ml of remaining 7.0ml @21:37:11.94 Intake.select :open @21:37:14.16 Intake @15.9psia, vacuum from 9.4 to 13.4psi @21:37:14.18 SS.reconfigure SSinhale.with maxSpeed: 150 @21:37:14.27 SS.seek 7ml @21:37:19.78 Stroke completed -- waiting for pressure to equalize ... @21:37:24.65 GoodNews.email "At 21:36:38 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:105\n{3 => 22, 4 => 22, 5 => 22}\nCan@21:36:38, 19.4C, 31% humidity, 14.7psia, 11.962V, 0.834A, 0.834A avg, 9.97W",Subject: "Started 18tun1 phase mbaPhase" @21:37:38.26
Intake.select :close @21:37:38.45 SSV.dial :exhaust @21:37:39.34 Exhaust.select :open @21:37:39.48 SS.reconfigure SSexhale @21:37:39.56 SS.seek 0ml @21:37:40.10 BACfans.email "Started mfb in tube 2 at 21:36:38CEST15-Sep-18\n{3 => 22, 4 => 22, 5 => 22}\nCan@21:36:38, 19.4C, 31% humidity, 14.7psia, 11.962V, 0.834A, 0.834A avg, 9.97W",Subject: "pcrBAC sampling at most 200ml, wcr at most 200ml" @21:37:41.27 delay 1 second @21:37:42.28 AFV.select :holdingCoil @21:37:42.41 delay 1 second @21:37:43.82
Exhaust.select :close @21:37:43.92 ATV.dial :waste @21:37:44.00
Sampling 200.0ml @21:37:44.01 Intake.select :close @21:37:44.04 Exhaust.select :close @21:37:44.07 SS.reconfigure SSconfig @21:37:44.15 SS.hold @21:37:44.43 CTV.dial :intake @21:37:44.45 SSV.dial CBV @21:37:44.55
CBV.dial SSV @21:37:45.00 AS.reconfigure ASnorm @21:37:45.07 AS.home.seek :empty @21:37:45.44
Allowing 130s for 25.0ml of remaining 200.0ml @21:37:45.49 Intake.select :open @21:37:47.73 Intake @15.8psia, vacuum from 9.3 to 13.3psi @21:37:47.75 SS.reconfigure SSinhale.with maxSpeed: 150 @21:37:47.85 SS.seek 25ml @21:37:50.95 Vacuum too high -- waiting up to 40s @21:37:56.44 Intake @16.0psia, vacuum from 9.5 to 13.5psi @21:37:56.47 SS.reconfigure SSinhale.with maxSpeed: 135 @21:37:56.56 SS.seek 25ml @21:37:59.45 Vacuum too high -- waiting up to 40s @21:38:04.55 -> exit @21:38:04.58 Session Ended @21:38:04.95
Intake @15.9psia, vacuum from 9.4 to 13.4psi @21:38:04.97 SS.reconfigure SSinhale.with maxSpeed: 122 @21:38:05.02 SS.seek 25ml @21:38:07.77 Vacuum too high -- waiting up to 40s @21:38:13.19 Intake @15.8psia, vacuum from 9.3 to 13.3psi @21:38:13.20 SS.reconfigure SSinhale.with maxSpeed: 110 @21:38:13.25 SS.seek 25ml @21:38:15.73 Vacuum too high -- waiting up to 40s @21:38:21.41 SS.reconfigure SSinhale.with maxSpeed: 99 @21:38:21.46 SS.seek 25ml @21:38:24.23 Vacuum too high -- waiting up to 40s @21:38:30.16 Intake @15.8psia, vacuum from 9.3 to 13.3psi @21:38:30.18 SS.reconfigure SSinhale.with maxSpeed: 89 @21:38:30.23 SS.seek 25ml @21:38:32.95 Vacuum too high -- waiting up to 40s @21:38:37.70 ATV.dial :waste @21:38:37.72 ARV.dial between carrier and air1 @21:38:37.80 ASV.dial :waste1 @21:38:38.81
Intake @15.8psia, vacuum from 9.3 to 13.3psi @21:38:38.83 SS.reconfigure SSinhale.with maxSpeed: 80 @21:38:38.88 SS.seek 25ml @21:38:41.51 Vacuum too high -- waiting up to 40s @21:38:46.72 Intake @15.8psia, vacuum from 9.3 to 13.3psi @21:38:46.74 SS.reconfigure SSinhale.with maxSpeed: 72 @21:38:46.79 SS.seek 25ml @21:38:49.98 Vacuum too high -- waiting up to 40s @21:38:55.36 Intake @15.8psia, vacuum from 9.3 to 13.3psi @21:38:55.37 SS.reconfigure SSinhale.with maxSpeed: 65 @21:38:55.42 SS.seek 25ml @21:38:58.31 Stroke completed -- waiting for pressure to equalize ... @21:39:04.72 Intake.select :close @21:39:04.87 SSV.dial :exhaust @21:39:05.73 Exhaust.select :open @21:39:05.85 SS.reconfigure SSexhale @21:39:05.90 SS.seek 0ml @21:39:15.46 Exhaust.select :close @21:39:15.58 SSV.dial CBV @21:39:15.61
CBV.dial SSV @21:39:16.47 Allowing 130s for 25.0ml of remaining 175.0ml @21:39:16.48 Intake.select :open @21:39:18.65 Intake @15.8psia, vacuum from 9.3 to 13.3psi @21:39:18.66 SS.reconfigure SSinhale.with maxSpeed: 65 @21:39:18.71 SS.seek 25ml @21:39:22.89 Vacuum too high -- waiting up to 40s @21:39:27.33 SS.reconfigure SSinhale.with maxSpeed: 59 @21:39:27.38 SS.seek 25ml @21:39:31.51 Vacuum too high -- waiting up to 40s @21:39:36.15 Intake @15.8psia, vacuum from 9.3 to 13.3psi @21:39:36.17 SS.reconfigure SSinhale.with maxSpeed: 53 @21:39:36.22 SS.seek 25ml @21:39:40.55 Vacuum too high -- waiting up to 40s @21:39:44.75 Intake @15.6psia, vacuum from 9.1 to 13.1psi @21:39:44.77 SS.reconfigure SSinhale.with maxSpeed: 48 @21:39:44.82 SS.seek 25ml @21:39:49.26 Vacuum too high -- waiting up to 40s @21:39:53.63 SS.reconfigure SSinhale.with maxSpeed: 43 @21:39:53.68 SS.seek 25ml @21:40:00.64 Vacuum too high -- waiting up to 40s @21:40:05.23 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:40:05.25 SS.reconfigure SSinhale.with maxSpeed: 39 @21:40:05.30 SS.seek 25ml @21:40:14.98 Vacuum too high -- waiting up to 40s @21:40:20.13 Intake @15.6psia, vacuum from 9.1 to 13.1psi @21:40:20.15 SS.reconfigure SSinhale.with maxSpeed: 35 @21:40:20.20 SS.seek 25ml @21:40:28.63 Stroke completed -- waiting for pressure to equalize ... @21:40:35.01 Intake.select :close @21:40:35.16 SSV.dial :exhaust @21:40:36.01 Exhaust.select :open @21:40:36.13 SS.reconfigure SSexhale @21:40:36.18 SS.seek 0ml @21:40:45.88 Exhaust.select :close @21:40:46.00 SSV.dial CBV @21:40:46.03
CBV.dial SSV @21:40:46.89 Allowing 130s for 25.0ml of remaining 150.0ml @21:40:46.90 Intake.select :open @21:40:49.07 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:40:49.09 SS.reconfigure SSinhale.with maxSpeed: 35 @21:40:49.14 SS.seek 25ml @21:40:59.83 Vacuum too high -- waiting up to 40s @21:41:03.46 Intake @15.5psia, vacuum from 9.0 to 13.0psi @21:41:03.48 SS.reconfigure SSinhale.with maxSpeed: 32 @21:41:03.53 SS.seek 25ml @21:41:16.86 Vacuum too high -- waiting up to 40s @21:41:20.49 Intake @15.5psia, vacuum from 9.0 to 13.0psi @21:41:20.51 SS.reconfigure SSinhale.with maxSpeed: 29 @21:41:20.56 SS.seek 25ml @21:41:54.31 Stroke completed -- waiting for pressure to equalize ... @21:42:00.70 Intake.select :close @21:42:00.85 SSV.dial :exhaust @21:42:01.70 Exhaust.select :open @21:42:01.82 SS.reconfigure SSexhale @21:42:01.87 SS.seek 0ml @21:42:11.10 Exhaust.select :close @21:42:11.22 SSV.dial CBV @21:42:11.25
CBV.dial SSV @21:42:12.13 Allowing 130s for 25.0ml of remaining 125.0ml @21:42:12.14 Intake.select :open @21:42:14.31 Intake @15.6psia, vacuum from 9.1 to 13.1psi @21:42:14.33 SS.reconfigure SSinhale.with maxSpeed: 29 @21:42:14.38 SS.seek 25ml @21:43:15.51 Stroke completed -- waiting for pressure to equalize ... @21:43:21.23 Intake.select :close @21:43:21.38 SSV.dial :exhaust @21:43:22.23 Exhaust.select :open @21:43:22.35 SS.reconfigure SSexhale @21:43:22.40 SS.seek 0ml @21:43:32.20 Exhaust.select :close @21:43:32.32 SSV.dial CBV @21:43:32.35
CBV.dial SSV @21:43:33.23 Allowing 130s for 25.0ml of remaining 100.0ml @21:43:33.25 Intake.select :open @21:43:35.42 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:43:35.44 SS.reconfigure SSinhale.with maxSpeed: 29 @21:43:35.49 SS.seek 25ml @21:44:36.12 Stroke completed -- waiting for pressure to equalize ... @21:44:41.23 Intake.select :close @21:44:41.38 SSV.dial :exhaust @21:44:42.24 Exhaust.select :open @21:44:42.36 SS.reconfigure SSexhale @21:44:42.41 SS.seek 0ml @21:44:51.96 Exhaust.select :close @21:44:52.08 SSV.dial CBV @21:44:52.11
CBV.dial SSV @21:44:52.99 Allowing 130s for 25.0ml of remaining 75.0ml @21:44:53.00 Intake.select :open @21:44:55.64 Intake @15.6psia, vacuum from 9.1 to 13.1psi @21:44:55.66 SS.reconfigure SSinhale.with maxSpeed: 29 @21:44:55.71 SS.seek 25ml @21:45:56.27 Stroke completed -- waiting for pressure to equalize ... @21:46:00.79 Intake.select :close @21:46:00.94 SSV.dial :exhaust @21:46:01.79 Exhaust.select :open @21:46:01.93 SS.reconfigure SSexhale @21:46:01.98 SS.seek 0ml @21:46:11.23 Exhaust.select :close @21:46:11.35 SSV.dial CBV @21:46:11.38
CBV.dial SSV @21:46:12.25 Allowing 130s for 25.0ml of remaining 50.0ml @21:46:12.26 Intake.select :open @21:46:14.43 Intake @15.5psia, vacuum from 9.0 to 13.0psi @21:46:14.45 SS.reconfigure SSinhale.with maxSpeed: 29 @21:46:14.50 SS.seek 25ml @21:47:15.66 Stroke completed -- waiting for pressure to equalize ... @21:47:20.78 Intake.select :close @21:47:20.93 SSV.dial :exhaust @21:47:21.79 Exhaust.select :open @21:47:21.91 SS.reconfigure SSexhale @21:47:21.96 SS.seek 0ml @21:47:31.77 Exhaust.select :close @21:47:31.88 SSV.dial CBV @21:47:31.91
CBV.dial SSV @21:47:32.80 Allowing 130s for 25.0ml of remaining 25.0ml @21:47:32.81 Intake.select :open @21:47:34.98 Intake @15.5psia, vacuum from 9.0 to 13.0psi @21:47:35.00 SS.reconfigure SSinhale.with maxSpeed: 29 @21:47:35.05 SS.seek 24.99ml @21:48:35.63 Stroke completed -- waiting for pressure to equalize ... @21:48:40.10 Intake.select :close @21:48:40.25 SSV.dial :exhaust @21:48:41.14 Exhaust.select :open @21:48:41.25 SS.reconfigure SSexhale @21:48:41.30 SS.seek 0ml @21:48:50.77 Exhaust.select :close @21:48:50.89 Sampled 200.0ml @21:48:50.90 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @21:48:50.91 Intake.select :close @21:48:50.98 CSV.dial :waste @21:48:51.04 CS.seek 0ml @21:48:51.16 SSV.dial CBV,avoiding: :exhaust @21:48:51.19
CBV.dial SSV @21:48:51.26 CSV.dial :air @21:48:52.10 CS.seek 10ml @21:48:53.33
CTV.dial :air @21:48:54.20 delay 15 seconds @21:49:09.22 SSV.dial :spit,avoiding: :exhaust @21:49:10.72 CTV.dial CSV,avoiding: :intake @21:49:10.76
SS.seek 0ml @21:49:10.91 SSV.dial CBV,avoiding: :exhaust @21:49:18.92 CBV.dial between SSV and waste2 @21:49:19.35 CTV.dial CSV,avoiding: :air @21:49:19.38
CSV.dial CTV @21:49:19.90 CS.reconfigure CSpressurize @21:49:19.95 CS.seek 0ml @21:49:46.13 CSV.dial :air @21:49:47.14 CS.reconfigure CSfast @21:49:47.19 CS.seek 10ml @21:50:13.76 CSV.dial between air and CTV @21:50:14.13 CS.seek 2.496ml @21:50:34.20 CTV.dial CSV,avoiding: :air @21:50:34.23
CSV.dial CTV @21:50:34.57 CS.reconfigure CSpressurize @21:50:34.63 CS.seek 0ml @21:50:38.22 BubblePoint: Intake @69.7psia, Outlet @14.1psia @21:50:38.23 CBV.dial SSV @21:50:38.61 SS.reconfigure SSconfig @21:50:38.66 SS.seek 10ml @21:50:44.72 delay 15 seconds @21:50:59.86 Evacuated: Intake @32.8psia, Outlet @14.3psia @21:50:59.87 SSV.dial :spit,avoiding: :exhaust @21:51:01.38 SS.reconfigure SSexhale @21:51:01.43 SS.seek 0ml @21:51:06.49 SS.reconfigure SSfast @21:51:06.55 SS.seek 5ml @21:51:09.41 SSV.dial CBV,avoiding: :exhaust @21:51:09.44
CBV.dial SSV @21:51:10.95 CTV.dial :air,avoiding: :intake @21:51:12.48 CSV.dial CTV @21:51:12.52
SS.seek 10ml @21:51:13.51 CSV.dial :air @21:51:14.04 CS.reconfigure CSfast @21:51:14.09 CS.seek 0ml @21:51:15.07
SSV.dial :spit,avoiding: :exhaust @21:51:16.58 SS.reconfigure SSexhale @21:51:16.63 SS.seek 0ml @21:51:18.69 CS.coast @21:51:21.63
SS.reconfigure SSfast @21:51:21.69 SS.seek 5ml @21:51:24.53 SSV.dial CBV,avoiding: :exhaust @21:51:24.55
CBV.dial SSV @21:51:27.05 SSV.dial :spit,avoiding: :exhaust @21:51:28.56 SS.reconfigure SSconfig @21:51:28.61 SS.seek 0ml @21:51:31.63 SS.coast @21:51:31.67 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @21:51:31.68 Intake.select :close @21:51:31.77 CSV.dial :waste @21:51:31.91 SSV.dial CBV,avoiding: :exhaust @21:51:31.96
CBV.dial SSV @21:51:32.68 CS.seek 0ml @21:51:32.94 CSV.dial :air @21:51:33.45
CTV.dial :air @21:51:33.46 delay 15 seconds @21:51:33.92 CS.seek 10ml @21:51:48.48
SSV.dial :spit,avoiding: :exhaust @21:51:49.98 CTV.dial CSV,avoiding: :intake @21:51:50.02
SS.reconfigure SSexhale @21:51:50.12 SS.seek 0ml @21:51:50.19 SSV.dial CBV,avoiding: :exhaust @21:52:00.76 CBV.dial between SSV and waste2 @21:52:01.20 CSV.dial between air and CTV @21:52:01.57 CS.seek 8.475ml @21:52:06.51 CTV.dial CSV,avoiding: :air @21:52:06.54
CSV.dial CTV @21:52:06.89 CS.reconfigure CSpressurize @21:52:06.94 CS.seek 0ml @21:52:29.16 CSV.dial :air @21:52:29.68 CS.reconfigure CSfast @21:52:29.73 CS.seek 10ml @21:52:56.07 CSV.dial between air and CTV @21:52:56.43 CS.seek 3.16ml @21:53:14.83 CTV.dial CSV,avoiding: :air @21:53:14.86
CSV.dial CTV @21:53:15.57 CS.reconfigure CSpressurize @21:53:15.70 CS.seek 0ml @21:53:23.82 BubblePoint: Intake @68.7psia, Outlet @13.5psia @21:53:23.83 CBV.dial SSV @21:53:24.20 SS.reconfigure SSconfig @21:53:24.25 SS.seek 10ml @21:53:29.67 delay 15 seconds @21:53:44.81 Evacuated: Intake @35.4psia, Outlet @17.0psia @21:53:44.82 SSV.dial :spit,avoiding: :exhaust @21:53:46.33 SS.reconfigure SSexhale @21:53:46.38 SS.seek 0ml @21:53:51.68 SS.reconfigure SSfast @21:53:51.73 SS.seek 5ml @21:53:54.33 SSV.dial CBV,avoiding: :exhaust @21:53:54.36
CBV.dial SSV @21:53:55.87 CTV.dial :air,avoiding: :intake @21:53:57.40 CSV.dial CTV @21:53:57.44
SS.seek 10ml @21:53:58.44 CSV.dial :air @21:53:58.95 CS.reconfigure CSfast @21:53:59.00 CS.seek 0ml @21:54:00.19
SSV.dial :spit,avoiding: :exhaust @21:54:00.72 CS.coast @21:54:01.69
SS.reconfigure SSexhale @21:54:01.75 SS.seek 0ml @21:54:06.79 SS.reconfigure SSfast @21:54:06.84 SS.seek 5ml @21:54:09.87 SSV.dial CBV,avoiding: :exhaust @21:54:09.90
CBV.dial SSV @21:54:12.40 SSV.dial :spit,avoiding: :exhaust @21:54:13.91 SS.reconfigure SSconfig @21:54:13.96 SS.seek 0ml @21:54:17.00 SS.coast @21:54:17.04 Lysing: Adding 1.5ml lysis @21:54:17.06 CBV.dial between CSV and waste1,avoiding: :DBV @21:54:18.77 CTV.dial CSV @21:54:20.28 CSV.dial CSR @21:54:21.18 Pulling 1ml of CSR.lysis @21:54:21.19 CSR.select :lysis @21:54:21.30 CS.reconfigure CSconfig @21:54:21.35 CS.seek 1ml @21:54:24.86 delay 5 seconds @21:54:29.88 CSR.select :air @21:54:30.05 CS.seek 6ml @21:54:43.91 delay 2 seconds @21:54:45.93 CSV.dial :waste1 @21:54:47.42 CS.reconfigure CSfast @21:54:47.47 CS.seek :empty @21:55:03.50 CSV.dial CSR @21:55:05.04 Pulling 1.5ml of CSR.lysis @21:55:05.05 CSR.select :lysis @21:55:05.16 CS.reconfigure CSconfig @21:55:05.21 CS.seek 1.5ml @21:55:10.17 delay 5 seconds @21:55:15.20 CSR.select :air @21:55:15.37 CS.seek 4.501ml @21:55:24.11 delay 2 seconds @21:55:26.12 CSV.dial CTV @21:55:26.64 CS.seek 1.275ml @21:55:35.92 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @21:55:35.94 CH.seek 85C,8:00 @22:03:36.08 Thermal::Error in MAIN -- Collection Puck Heater could not reach 85C in 8:00 @22:03:36.10 Waiting to Resume from Checkpoint "Collection Puck Heater could not reach 85C in 8:00" @22:03:36.11 BadNews.email "Collection Puck Heater could not reach 85C in 8:00",Subject: "Thermal::Error in MAIN" @23:16:55.22 Session Begun @23:17:04.45 -> CH @23:17:13.74 -> PH @23:19:25.86 -> exit @23:19:25.88 Session Ended @10:09:33.42CEST16-Sep-18 Session Begun @10:09:38.09 -> showlog 100 @10:09:57.93 -> CSV @10:10:14.22 -> CTV @10:10:25.31 -> CVV @10:10:25.33 NameError in bkha -- uninitialized constant CVV @10:10:34.33 -> CBV @10:10:44.69 -> CBV.waste1 @10:10:44.71 CBV.dial :waste1 @10:10:56.75 -> CS.empty @10:10:56.78 CS.seek :empty @10:11:19.07 -> puckEvac 15 @10:11:19.11 Evacuating Puck (bubblePt=15psi, evacVolume=10ml, delay 15 seconds) @10:11:19.13 Intake.select :close @10:11:19.24 CSV.dial :waste @10:11:19.41 SSV.dial CBV,avoiding: :exhaust @10:11:19.44 CBV.dial SSV @10:11:20.48 CS.reconfigure CSfast @10:11:20.55 CS.seek 0ml @10:11:21.05 CTV.dial :air @10:11:21.07 CSV.dial :air @10:11:21.99 CS.seek 10ml @10:11:22.65 delay 15 seconds @10:11:37.67 SSV.dial :spit,avoiding: :exhaust @10:11:39.20 CTV.dial CSV,avoiding: :intake @10:11:39.24 SS.reconfigure SSexhale @10:11:39.37 SS.seek 0ml @10:11:39.46 SSV.dial CBV,avoiding: :exhaust @10:11:48.88 CBV.dial between SSV and waste2 @10:11:49.34 CTV.dial CSV,avoiding: :air @10:11:49.37 CSV.dial CTV @10:11:49.92 CS.reconfigure CSpressurize @10:11:50.00 CS.seek 0ml @10:12:11.06 BubblePoint: Intake @32.4psia, Outlet @17.4psia @10:12:11.08 CBV.dial SSV @10:12:11.48 SS.reconfigure SSconfig @10:12:11.55 SS.seek 10ml @10:12:17.21 delay 15 seconds @10:12:32.36 Evacuated: Intake @30.9psia, Outlet @4.9psia @10:12:32.37 SSV.dial :spit,avoiding: :exhaust @10:12:33.90 SS.reconfigure SSexhale @10:12:33.99 SS.seek 0ml @10:12:39.02 SS.reconfigure SSfast @10:12:39.10 SS.seek 5ml @10:12:42.15 SSV.dial CBV,avoiding: :exhaust @10:12:42.17 CBV.dial SSV @10:12:43.71 CTV.dial :air,avoiding: :intake @10:12:45.23 CSV.dial CTV @10:12:45.28 SS.seek 10ml @10:12:46.28 CSV.dial :air @10:12:46.93 CS.reconfigure CSfast @10:12:47.00 CS.seek 0ml @10:12:48.03 SSV.dial :spit,avoiding: :exhaust @10:12:49.57 SS.reconfigure SSexhale @10:12:49.66 SS.seek 0ml @10:12:53.28 CS.coast @10:12:54.76 SS.reconfigure SSfast @10:12:54.85 SS.seek 5ml @10:12:57.65 SSV.dial CBV,avoiding: :exhaust @10:12:57.68 CBV.dial SSV @10:13:00.21 SSV.dial :spit,avoiding: :exhaust @10:13:01.73 SS.reconfigure SSconfig @10:13:01.81 SS.seek 0ml @10:13:05.03 SS.coast @10:13:28.88 -> CC.unloadPuck 1,1 @10:13:28.98 Puck::Warning in bkha -- Unloading sh1 puck from tube 2 to Storage Carousel @10:13:29.01 Storing 1 puck in Collection Clamp to tube 1 @10:13:29.23 SC.reconfigure SCtight @10:13:29.30 SC.seek 1 @10:13:31.47 SC.reconfigure SCloose @10:13:31.74 Elbow.reconfigure TightElbow @10:13:31.81 Elbow.seek 1 @10:13:33.73 Forearm.reconfigure TightForearm @10:13:33.80 Forearm.seek 1 + 700 counts @10:13:34.83 SE.reconfigure SEpick @10:13:34.93 SE.seek beamBreak @10:13:38.42 SE.configure SEconfig @10:13:38.50 SE.seek :place @10:13:39.19 Forearm.reconfigure LooseForearm @10:13:39.26 Forearm.seek :retract @10:13:40.21 Elbow.seek CC @10:13:41.90 Elbow.reconfigure LooseElbow @10:13:42.03 Forearm.seek CC @10:13:43.05 Hand.close @10:13:43.85 CC.seek :open @10:13:48.39 Forearm.seek :retract @10:13:49.42 Elbow.reconfigure TightElbow @10:13:49.49 Elbow.seek 1 @10:13:51.41 Elbow.reconfigure LimpElbow @10:13:51.49 Forearm.seek 1 @10:13:52.57 Elbow.hold @10:13:52.66 Elbow.reconfigure TightElbow @10:13:52.73 Hand.open @10:13:53.61 Forearm.force -20 @10:13:53.68 Forearm.seek 1 + 347 counts @10:13:54.03 Forearm.reconfigure TightForearm @10:13:54.11 Forearm.seek 1 + 700 counts @10:13:54.51 SE.seek :down @10:13:55.67 Forearm.reconfigure LooseForearm @10:13:55.74 Forearm.seek :retract @10:13:57.36 SE.coast @10:13:57.42 SC.coast @10:14:34.72 -> abort! "heater down, fixing heater" @10:14:34.75 CmdServer::Client::Error in bkha -- Aborting Mission: heater down, fixing heater @10:14:34.82 BadNews.email "heater down, fixing heater",Subject: "18tun1 ABORTED!" @10:14:44.87 Session Ended @18:33:16.39CEST16-Sep-18
>RESET< ESP2 v3.30 I2C Gateway v3.06 for core bus @18:33:18.59 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @18:33:18.92 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @18:33:24.50 Polling Can every 10:00 @18:33:24.53 Storage::Warning in MAIN -- Uncleanly Exited @18:33:24.54 Storage::Warning in MAIN -- Hand.holds FlushPuck @18:33:24.55 ESP.ready! will restore puck positions @18:33:24.59 Loading /home/esp/esp2/mission/18tun1.rb mission @18:33:24.66 Preparing to sleep until +0:00:15 @18:33:24.71 GoodNews.email "Starting 18tun1 w/pucks in tube 2\n{3 => 22, 4 => 22, 5 => 22}\nCan@18:33:24, 23.3C, 33% humidity, 14.7psia, 12.075V, 0.367A, 0.367A avg, 4.43W",Subject: "18tun1 begun" @18:33:24.77 Shutdown @18:33:24.80
Asleep til +0:00:15 @18:33:24.82 Continuing at 18:33 @18:33:26.68 Session Begun @18:33:32.62 -> showlog 100 @18:33:34.32 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:105\n{3 => 22, 4 => 22, 5 => 22}\nCan@18:33:24, 23.3C, 33% humidity, 14.7psia, 12.075V, 0.367A, 0.367A avg, 4.43W",Subject: "Asleep until 18:33" @18:33:39.64
Powering core bus @18:33:44.59 Gate.power :raw,:ON @18:33:45.32 SPE.coast @18:33:45.41 SPE.configure SPEconfig @18:33:45.53 AS.configure ASconfig @18:33:45.67 SS.configure SSconfig @18:33:45.80 CC.configure CCconfig @18:33:45.93 CS.configure CSconfig @18:33:46.05 PC.configure PCconfig @18:33:46.18 PS.configure PSconfig @18:33:46.32 Forearm.configure LooseForearm @18:33:46.45 Elbow.configure LooseElbow @18:33:46.60 SC.configure SCloose @18:33:46.73 SE.configure SEconfig @18:33:46.79 CH.coast @18:33:46.90 CH.configure CHconfig @18:33:46.95 PH.coast @18:33:47.03 PH.configure PHconfig @18:33:47.09 PCR.off @18:33:47.15 PCR.pidtemp= 50,0.1,0,300,25,25 @18:33:47.20 PCR.caltemp= 2.7073,0.0373,0 @18:33:47.23 All dwarf controllers running firmware version 3.75 @18:33:47.39 Valve.reset @18:33:47.59 PCR.off @18:33:47.65 PCR.pidtemp= 50,0.1,0,300,25,25 @18:33:47.72 PCR.caltemp= 2.7073,0.0373,0 @18:33:47.95 Intake.select :close @18:33:48.00 PSV.dial :air @18:33:48.03 Exhaust.select :close @18:33:48.05 CTV.dial :air @18:33:48.25 CSV.dial :waste1 @18:33:48.31 PS.reconfigure PSconfig @18:33:48.46 PS.home.seek 0ml @18:33:48.50
Forearm.reconfigure LooseForearm @18:33:48.66 SSV.dial :spit,avoiding: :exhaust @18:33:48.68
Forearm.home.seek :retract @18:33:48.91 CS.reconfigure CSfast @18:33:48.95 SS.reconfigure SSconfig @18:33:49.06 CS.home.seek 0ml @18:33:49.11 SS.home.seek 0ml @18:33:49.50 Hand.close @18:33:49.55
Elbow.jog 1500 @18:33:50.31 Elbow.reconfigure LooseElbow @18:33:50.38 Elbow.home.seek :clear @18:33:53.74 SE.reconfigure SEconfig @18:33:53.82 SE.home.seek :down @18:33:54.52 SC.reconfigure SCsloppy @18:33:54.60 SC.home.seek :initial @18:33:57.81 PS.coast @18:33:58.77
Forearm.seek :retract @18:33:58.88 Elbow.seek :hammer @18:34:00.08 Forearm.reconfigure TightForearm @18:34:00.16 Forearm.seek 5 + 350 counts @18:34:01.25 Forearm.seek :retract @18:34:02.35 Hand.holds FlushPuck @18:34:02.37 Garaging FlushPuck in Hand @18:34:02.54 Elbow.reconfigure TightElbow @18:34:02.62 Elbow.seek :garage @18:34:04.87 Elbow.reconfigure LooseElbow @18:34:05.00 Forearm.seek :garage @18:34:06.18 Hand.open @18:34:06.98 Forearm.reconfigure LooseForearm @18:34:07.06 Forearm.seek :retract @18:34:08.28 PC.reconfigure PCconfig @18:34:08.30
CC.reconfigure CCconfig @18:34:08.43 PC.home.seek :open @18:34:08.45
CC.home.seek :open @18:34:11.83 Phase [mbaPhase] @18:34:11.85 GoodNews.email "At 18:34:11 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tun1.rb:105\n{3 => 22, 4 => 22, 5 => 22}\nCan@18:34:11, 23.4C, 33% humidity, 14.7psia, 11.952V, 0.834A, 0.827A avg, 9.97W",Subject: "Started 18tun1 phase mbaPhase" @18:34:11.90CEST16-Sep-18
==================> STARTED MBAsuperphase (phase #1) <=== @18:34:12.02 Starting mfb @18:34:12.04 pcrBAC sampling at most 200ml, wcr at most 200ml @18:34:12.15 Loading sh1 puck from tube 2 into Collection Clamp @18:34:12.30 Readying the MFB @18:34:12.34 Homing MFB Syringe @18:34:12.36 AFV.select :holdingCoil @18:34:12.66 ATV.dial :air2 @18:34:12.84
SC.reconfigure SCloose @18:34:13.12 Elbow.reconfigure TightElbow @18:34:13.22 Elbow.seek 2 @18:34:13.32 AS.reconfigure ASconfig @18:34:13.38 AS.home.seek :home @18:34:15.29
Forearm.reconfigure TightForearm @18:34:15.37 Forearm.seek 2 + 700 counts @18:34:16.56 SE.reconfigure SEpick @18:34:16.66 SE.seek beamBreak @18:34:17.85 SE.configure SEconfig @18:34:18.29 Elbow.seek 2 @18:34:18.55 Forearm.seek 2 + 300 counts @18:34:18.83 Forearm.reconfigure LooseForearm @18:34:18.92 Hand.close @18:34:18.98 BACfans.email "Started mfb in tube 2 at 18:34:12CEST16-Sep-18\n{3 => 22, 4 => 22, 5 => 22}\nCan@18:34:11, 23.4C, 33% humidity, 14.7psia, 11.952V, 0.834A, 0.827A avg, 9.97W",Subject: "pcrBAC sampling at most 200ml, wcr at most 200ml" @18:34:19.08 AFV.select :carrierSolution @18:34:19.21 delay 2 seconds @18:34:19.67 SE.seek :down @18:34:19.93
Forearm.seek :retract @18:34:20.58 SE.coast @18:34:20.90
Elbow.seek CC @18:34:21.23 AS.seek 1ml @18:34:22.51
Elbow.reconfigure LooseElbow @18:34:22.66 Forearm.reconfigure TightForearm @18:34:22.74 Forearm.seek CC @18:34:24.11 CC.seek :closed @18:34:28.46 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.58 @18:34:28.47 Hand.open @18:34:29.36 Forearm.reconfigure LooseForearm @18:34:29.43 Forearm.seek :retract @18:34:30.42 SC.coast @18:34:30.48 collectSample 200ml w/bubble point of 55psi @18:34:30.50 Priming 7ml @18:34:30.52 Intake.select :close @18:34:30.54 Exhaust.select :close @18:34:30.58 SS.hold @18:34:30.88 Calibrating pressure and temperature sensors @18:34:30.90 CTV.dial :air @18:34:30.93 SSV.dial :spit,avoiding: :exhaust @18:34:31.00 SS.reconfigure SSfast @18:34:31.07 SS.seek 5ml @18:34:33.94 SSV.dial CBV @18:34:33.97
CBV.dial SSV @18:34:35.52 delay 3.8 seconds @18:34:39.48 SSV.dial :spit @18:34:40.99 SS.seek 0ml @18:34:43.79 SSV.dial CBV @18:34:45.29 CTV.dial :intake @18:34:46.18 SSV.dial CBV @18:34:46.21
CBV.dial SSV @18:34:46.25 Allowing 40s for 7.0ml of remaining 7.0ml @18:34:46.27 Intake.select :open @18:34:48.48 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:34:48.50 SS.reconfigure SSinhale.with maxSpeed: 150 @18:34:48.59 SS.seek 7ml @18:34:54.57 Stroke completed -- waiting for pressure to equalize ... @18:35:09.11 Intake.select :close @18:35:09.29 SSV.dial :exhaust @18:35:10.17 Exhaust.select :open @18:35:10.31 SS.reconfigure SSexhale @18:35:10.38 SS.seek 0ml @18:35:14.15 delay 1 second @18:35:14.70
Exhaust.select :close @18:35:14.84 Sampling 200.0ml @18:35:14.85 Intake.select :close @18:35:14.88 Exhaust.select :close @18:35:14.91 SS.reconfigure SSconfig @18:35:14.99 SS.hold @18:35:15.24 CTV.dial :intake @18:35:15.26 SSV.dial CBV @18:35:15.30
CBV.dial SSV @18:35:15.38 AFV.select :holdingCoil @18:35:15.52 delay 1 second @18:35:16.20
Allowing 130s for 25.0ml of remaining 200.0ml @18:35:16.22 Intake.select :open @18:35:16.53 ATV.dial :waste @18:35:17.62 AS.reconfigure ASnorm @18:35:17.69 AS.home.seek :empty @18:35:18.43
Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:35:18.45 SS.reconfigure SSinhale.with maxSpeed: 150 @18:35:18.54 SS.seek 25ml @18:35:22.22 Vacuum too high -- waiting up to 40s @18:35:27.14 SS.reconfigure SSinhale.with maxSpeed: 135 @18:35:27.21 SS.seek 25ml @18:35:30.41 Vacuum too high -- waiting up to 40s @18:35:35.04 SS.reconfigure SSinhale.with maxSpeed: 122 @18:35:35.11 SS.seek 25ml @18:35:37.84 Vacuum too high -- waiting up to 40s @18:35:41.95 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:35:41.97 SS.reconfigure SSinhale.with maxSpeed: 110 @18:35:42.06 SS.seek 25ml @18:35:44.97 Vacuum too high -- waiting up to 40s @18:35:48.86 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:35:48.88 SS.reconfigure SSinhale.with maxSpeed: 99 @18:35:48.98 SS.seek 25ml @18:35:52.16 Vacuum too high -- waiting up to 40s @18:35:56.58 SS.reconfigure SSinhale.with maxSpeed: 89 @18:35:56.66 SS.seek 25ml @18:36:00.63 Vacuum too high -- waiting up to 40s @18:36:05.51 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:36:05.53 SS.reconfigure SSinhale.with maxSpeed: 80 @18:36:05.63 SS.seek 25ml @18:36:08.81 Stroke completed -- waiting for pressure to equalize ... @18:36:10.36 ATV.dial :waste @18:36:10.38 ARV.dial between carrier and air1 @18:36:10.50 ASV.dial :waste1 @18:36:12.08
Intake.select :close @18:36:12.25 SSV.dial :exhaust @18:36:13.14 Exhaust.select :open @18:36:13.28 SS.reconfigure SSexhale @18:36:13.36 SS.seek 0ml @18:36:22.73 Exhaust.select :close @18:36:23.36 SSV.dial CBV @18:36:23.39
CBV.dial SSV @18:36:24.30 Allowing 130s for 25.0ml of remaining 175.0ml @18:36:24.31 Intake.select :open @18:36:26.52 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:36:26.54 SS.reconfigure SSinhale.with maxSpeed: 80 @18:36:26.62 SS.seek 25ml @18:36:32.34 Vacuum too high -- waiting up to 40s @18:36:36.45 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:36:36.47 SS.reconfigure SSinhale.with maxSpeed: 72 @18:36:36.56 SS.seek 25ml @18:36:41.81 Vacuum too high -- waiting up to 40s @18:36:45.96 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:36:45.98 SS.reconfigure SSinhale.with maxSpeed: 65 @18:36:46.07 SS.seek 25ml @18:36:52.84 Vacuum too high -- waiting up to 40s @18:36:57.46 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:36:57.48 SS.reconfigure SSinhale.with maxSpeed: 59 @18:36:57.58 SS.seek 25ml @18:37:05.09 Vacuum too high -- waiting up to 40s @18:37:09.43 SS.reconfigure SSinhale.with maxSpeed: 53 @18:37:09.51 SS.seek 25ml @18:37:15.42 Stroke completed -- waiting for pressure to equalize ... @18:37:20.55 Intake.select :close @18:37:20.72 SSV.dial :exhaust @18:37:21.59 Exhaust.select :open @18:37:21.73 SS.reconfigure SSexhale @18:37:21.80 SS.seek 0ml @18:37:31.28 Exhaust.select :close @18:37:31.43 SSV.dial CBV @18:37:31.46
CBV.dial SSV @18:37:32.36 Allowing 130s for 25.0ml of remaining 150.0ml @18:37:32.37 Intake.select :open @18:37:34.58 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:37:34.60 SS.reconfigure SSinhale.with maxSpeed: 53 @18:37:34.70 SS.seek 25ml @18:37:43.44 Vacuum too high -- waiting up to 40s @18:37:46.56 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:37:46.58 SS.reconfigure SSinhale.with maxSpeed: 48 @18:37:46.67 SS.seek 25ml @18:38:02.78 Vacuum too high -- waiting up to 40s @18:38:07.39 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:38:07.41 SS.reconfigure SSinhale.with maxSpeed: 43 @18:38:07.50 SS.seek 25ml @18:38:21.90 Stroke completed -- waiting for pressure to equalize ... @18:38:27.03 Intake.select :close @18:38:27.20 SSV.dial :exhaust @18:38:28.09 Exhaust.select :open @18:38:28.22 SS.reconfigure SSexhale @18:38:28.30 SS.seek 0ml @18:38:38.10 Exhaust.select :close @18:38:38.24 SSV.dial CBV @18:38:38.27
CBV.dial SSV @18:38:39.16 Allowing 130s for 25.0ml of remaining 125.0ml @18:38:39.17 Intake.select :open @18:38:41.38 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:38:41.40 SS.reconfigure SSinhale.with maxSpeed: 43 @18:38:41.50 SS.seek 25ml @18:39:02.41 Vacuum too high -- waiting up to 40s @18:39:05.54 Intake @15.3psia, vacuum from 8.8 to 12.8psi @18:39:05.56 SS.reconfigure SSinhale.with maxSpeed: 39 @18:39:05.65 SS.seek 25ml @18:39:28.48 Stroke completed -- waiting for pressure to equalize ... @18:39:32.93 Intake.select :close @18:39:33.11 SSV.dial :exhaust @18:39:33.98 Exhaust.select :open @18:39:34.12 SS.reconfigure SSexhale @18:39:34.20 SS.seek 0ml @18:39:44.01 Exhaust.select :close @18:39:44.15 SSV.dial CBV @18:39:44.18
CBV.dial SSV @18:39:45.08 Allowing 130s for 25.0ml of remaining 100.0ml @18:39:45.09 Intake.select :open @18:39:47.30 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:39:47.32 SS.reconfigure SSinhale.with maxSpeed: 39 @18:39:47.41 SS.seek 25ml @18:40:32.59 Stroke completed -- waiting for pressure to equalize ... @18:40:37.08 Intake.select :close @18:40:37.26 SSV.dial :exhaust @18:40:38.12 Exhaust.select :open @18:40:38.26 SS.reconfigure SSexhale @18:40:38.33 SS.seek 0ml @18:40:47.98 Exhaust.select :close @18:40:48.12 SSV.dial CBV @18:40:48.15
CBV.dial SSV @18:40:49.04 Allowing 130s for 25.0ml of remaining 75.0ml @18:40:49.05 Intake.select :open @18:40:51.26 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:40:51.28 SS.reconfigure SSinhale.with maxSpeed: 39 @18:40:51.38 SS.seek 25ml @18:41:37.43 Stroke completed -- waiting for pressure to equalize ... @18:41:42.54 Intake.select :close @18:41:42.72 SSV.dial :exhaust @18:41:43.60 Exhaust.select :open @18:41:43.74 SS.reconfigure SSexhale @18:41:43.82 SS.seek 0ml @18:41:53.13 Exhaust.select :close @18:41:53.27 SSV.dial CBV @18:41:53.30
CBV.dial SSV @18:41:54.19 Allowing 130s for 25.0ml of remaining 50.0ml @18:41:54.20 Intake.select :open @18:41:56.41 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:41:56.44 SS.reconfigure SSinhale.with maxSpeed: 39 @18:41:56.53 SS.seek 25ml @18:42:42.54 Stroke completed -- waiting for pressure to equalize ... @18:42:47.64 Intake.select :close @18:42:47.81 SSV.dial :exhaust @18:42:48.68 Exhaust.select :open @18:42:48.83 SS.reconfigure SSexhale @18:42:48.91 SS.seek 0ml @18:42:58.60 Exhaust.select :close @18:42:58.74 SSV.dial CBV @18:42:58.77
CBV.dial SSV @18:42:59.63 Allowing 130s for 25.0ml of remaining 25.0ml @18:42:59.65 Intake.select :open @18:43:01.86 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:43:01.88 SS.reconfigure SSinhale.with maxSpeed: 39 @18:43:01.98 SS.seek 24.99ml @18:43:07.65 -> exit @18:43:07.67 Session Ended @18:43:47.32
Stroke completed -- waiting for pressure to equalize ... @18:43:51.79 Intake.select :close @18:43:51.94 SSV.dial :exhaust @18:43:52.79 Exhaust.select :open @18:43:52.91 SS.reconfigure SSexhale @18:43:52.96 SS.seek 0ml @18:44:02.66 Exhaust.select :close @18:44:02.78 Sampled 200.0ml @18:44:02.79 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @18:44:02.80 Intake.select :close @18:44:02.88 CSV.dial :waste @18:44:02.96 CS.seek 0ml @18:44:03.04 SSV.dial CBV,avoiding: :exhaust @18:44:03.07
CBV.dial SSV @18:44:03.14 CSV.dial :air @18:44:04.07 CS.seek 10ml @18:44:05.22
CTV.dial :air @18:44:06.06 delay 15 seconds @18:44:21.08 SSV.dial :spit,avoiding: :exhaust @18:44:22.58 CTV.dial CSV,avoiding: :intake @18:44:22.62
SS.seek 0ml @18:44:22.76 SSV.dial CBV,avoiding: :exhaust @18:44:30.90 CBV.dial between SSV and waste2 @18:44:31.33 CTV.dial CSV,avoiding: :air @18:44:31.36
CSV.dial CTV @18:44:32.01 CS.reconfigure CSpressurize @18:44:32.06 CS.seek 0ml @18:44:58.27 CSV.dial :air @18:44:59.29 CS.reconfigure CSfast @18:44:59.34 CS.seek 10ml @18:45:25.68 CSV.dial between air and CTV @18:45:26.05 CS.seek 2.615ml @18:45:45.71 CTV.dial CSV,avoiding: :air @18:45:45.74
CSV.dial CTV @18:45:46.20 CS.reconfigure CSpressurize @18:45:46.25 CS.seek 0ml @18:45:50.78 BubblePoint: Intake @70.4psia, Outlet @14.9psia @18:45:50.79 CBV.dial SSV @18:45:51.15 SS.reconfigure SSconfig @18:45:51.20 SS.seek 10ml @18:45:57.05 delay 15 seconds @18:46:12.20 Evacuated: Intake @36.9psia, Outlet @13.1psia @18:46:12.21 SSV.dial :spit,avoiding: :exhaust @18:46:13.71 SS.reconfigure SSexhale @18:46:13.76 SS.seek 0ml @18:46:19.20 SS.reconfigure SSfast @18:46:19.25 SS.seek 5ml @18:46:21.85 SSV.dial CBV,avoiding: :exhaust @18:46:21.88
CBV.dial SSV @18:46:23.39 CTV.dial :air,avoiding: :intake @18:46:24.90 CSV.dial CTV @18:46:24.94
SS.seek 10ml @18:46:25.94 CSV.dial :air @18:46:26.45 CS.reconfigure CSfast @18:46:26.50 CS.seek 0ml @18:46:27.68
SSV.dial :spit,avoiding: :exhaust @18:46:29.19 SS.reconfigure SSexhale @18:46:29.24 SS.seek 0ml @18:46:30.28 CS.coast @18:46:34.34
SS.reconfigure SSfast @18:46:34.39 SS.seek 5ml @18:46:37.27 SSV.dial CBV,avoiding: :exhaust @18:46:37.30
CBV.dial SSV @18:46:39.81 SSV.dial :spit,avoiding: :exhaust @18:46:41.30 SS.reconfigure SSconfig @18:46:41.35 SS.seek 0ml @18:46:44.38 SS.coast @18:46:44.42 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @18:46:44.43 Intake.select :close @18:46:44.51 CSV.dial :waste @18:46:44.64 SSV.dial CBV,avoiding: :exhaust @18:46:44.67
CBV.dial SSV @18:46:45.42 CS.seek 0ml @18:46:45.49 CSV.dial :air @18:46:46.16
CTV.dial :air @18:46:46.18 delay 15 seconds @18:46:46.34 CS.seek 10ml @18:47:01.20
SSV.dial :spit,avoiding: :exhaust @18:47:02.70 CTV.dial CSV,avoiding: :intake @18:47:02.74
SS.reconfigure SSexhale @18:47:02.85 SS.seek 0ml @18:47:03.29 SSV.dial CBV,avoiding: :exhaust @18:47:12.93 CBV.dial between SSV and waste2 @18:47:13.37 CSV.dial between air and CTV @18:47:13.73 CS.seek 8.219ml @18:47:19.43 CTV.dial CSV,avoiding: :air @18:47:19.46
CSV.dial CTV @18:47:19.79 CS.reconfigure CSpressurize @18:47:19.84 CS.seek 0ml @18:47:41.42 CSV.dial :air @18:47:41.93 CS.reconfigure CSfast @18:47:41.98 CS.seek 10ml @18:48:08.24 CSV.dial between air and CTV @18:48:08.62 CS.seek 3.147ml @18:48:27.00 CTV.dial CSV,avoiding: :air @18:48:27.03
CSV.dial CTV @18:48:27.87 CS.reconfigure CSpressurize @18:48:28.00 CS.seek 0ml @18:48:36.30 BubblePoint: Intake @69.7psia, Outlet @14.2psia @18:48:36.31 CBV.dial SSV @18:48:36.68 SS.reconfigure SSconfig @18:48:36.73 SS.seek 10ml @18:48:42.49 delay 15 seconds @18:48:57.64 Evacuated: Intake @40.0psia, Outlet @16.0psia @18:48:57.66 SSV.dial :spit,avoiding: :exhaust @18:48:59.16 SS.reconfigure SSexhale @18:48:59.21 SS.seek 0ml @18:49:04.29 SS.reconfigure SSfast @18:49:04.34 SS.seek 5ml @18:49:07.41 SSV.dial CBV,avoiding: :exhaust @18:49:07.44
CBV.dial SSV @18:49:08.96 CTV.dial :air,avoiding: :intake @18:49:10.48 CSV.dial CTV @18:49:10.52
SS.seek 10ml @18:49:11.51 CSV.dial :air @18:49:12.14 CS.reconfigure CSfast @18:49:12.19 CS.seek 0ml @18:49:13.18
SSV.dial :spit,avoiding: :exhaust @18:49:13.68 CS.coast @18:49:14.69
SS.reconfigure SSexhale @18:49:14.74 SS.seek 0ml @18:49:20.00 SS.reconfigure SSfast @18:49:20.05 SS.seek 5ml @18:49:22.97 SSV.dial CBV,avoiding: :exhaust @18:49:23.00
CBV.dial SSV @18:49:25.52 SSV.dial :spit,avoiding: :exhaust @18:49:27.03 SS.reconfigure SSconfig @18:49:27.08 SS.seek 0ml @18:49:30.43 SS.coast @18:49:30.47 Lysing: Adding 1.5ml lysis @18:49:30.48 CBV.dial between CSV and waste1,avoiding: :DBV @18:49:32.17 CTV.dial CSV @18:49:33.66 CSV.dial CSR @18:49:34.56 Pulling 1ml of CSR.lysis @18:49:34.57 CSR.select :lysis @18:49:34.68 CS.reconfigure CSconfig @18:49:34.74 CS.seek 1ml @18:49:38.27 delay 5 seconds @18:49:43.30 CSR.select :air @18:49:43.48 CS.seek 6ml @18:49:57.30 delay 2 seconds @18:49:59.32 CSV.dial :waste1 @18:50:00.79 CS.reconfigure CSfast @18:50:00.84 CS.seek :empty @18:50:16.86 CSV.dial CSR @18:50:18.40 Pulling 1.5ml of CSR.lysis @18:50:18.41 CSR.select :lysis @18:50:18.52 CS.reconfigure CSconfig @18:50:18.58 CS.seek 1.5ml @18:50:23.50 delay 5 seconds @18:50:28.52 CSR.select :air @18:50:28.69 CS.seek 4.5ml @18:50:37.36 delay 2 seconds @18:50:39.38 CSV.dial CTV @18:50:39.90 CS.seek 1.275ml @18:50:49.09 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @18:50:49.11 CH.seek 85C,8:00 @18:53:56.04 CH.hold 5:00,85C @18:58:56.22 Collection Puck Heater at 85C after lysing @18:58:56.24 CS.seek 0ml @18:59:00.47 CTV.dial between spare and air,avoiding: :intake @18:59:01.65 CSV.dial :air @18:59:02.16 CS.seek 5ml @18:59:15.96 CSV.dial CTV @18:59:16.49 delay 2 seconds @18:59:18.51 CSV.dial :air @18:59:19.03 delay 2 seconds @18:59:21.04 CSV.dial CTV @18:59:21.57 delay 2 seconds @18:59:23.58 CSV.dial :air @18:59:24.21 delay 2 seconds @18:59:26.24 CS.seek 0ml @18:59:40.00 Recovering Lysate into Collection syringe @18:59:40.01 CSV.dial CBV @18:59:41.29 CBV.dial CSV @18:59:41.71 CS.seek 5ml @18:59:55.52 delay 5 seconds @19:00:00.54 CTV.dial :air @19:00:01.14 CS.seek 7ml @19:00:07.18 delay 5 seconds @19:00:12.20 CSV.dial :air2 @19:00:12.72 CS.seek 10ml @19:00:21.46 CSV.dial between air and CTV,avoiding: CBV @19:00:22.78 Lysing Complete @19:00:22.86 Storing sh1 puck in Collection Clamp to tube 1 @19:00:23.05 SC.reconfigure SCtight @19:00:23.10 SC.seek 1 @19:00:25.24 SC.reconfigure SCloose @19:00:25.47 Elbow.reconfigure TightElbow @19:00:25.52 Elbow.seek 1 @19:00:27.51 Forearm.reconfigure TightForearm @19:00:27.56 Forearm.seek 1 + 700 counts @19:00:28.67 SE.reconfigure SEpick @19:00:28.75 SE.seek beamBreak @19:00:31.71 SE.configure SEconfig @19:00:31.76 SE.seek :place @19:00:32.41 Forearm.reconfigure LooseForearm @19:00:32.46 Forearm.seek :retract @19:00:33.37 Elbow.seek CC @19:00:35.11 Elbow.reconfigure LooseElbow @19:00:35.22 Forearm.seek CC @19:00:36.16 Hand.close @19:00:37.36 CC.seek :open @19:00:41.99 Forearm.seek :retract @19:00:42.97 Elbow.reconfigure TightElbow @19:00:43.02 Elbow.seek 1 @19:00:44.96 Elbow.reconfigure LimpElbow @19:00:45.02 Forearm.seek 1 @19:00:46.08 Elbow.hold @19:00:46.15 Elbow.reconfigure TightElbow @19:00:46.20 Hand.open @19:00:47.07 Forearm.force -20 @19:00:47.11 Forearm.seek 1 + 347 counts @19:00:47.44 Forearm.reconfigure TightForearm @19:00:47.49 Forearm.seek 1 + 700 counts @19:00:47.90 SE.seek :down @19:00:49.07
Forearm.reconfigure LooseForearm @19:00:49.12 Forearm.seek :retract @19:00:50.71 SE.coast @19:00:50.75
SC.coast @19:00:50.79 Tranferring lysate to PRV @19:00:50.80 CSV.dial :air @19:00:51.14 CS.seek 10ml @19:00:51.22 CSV.dial between air and CTV @19:00:51.58 AIV.dial :bypass @19:00:51.68 Beginning samplePassC @19:00:51.69 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:00:51.77 Hand.open @19:00:52.21 Elbow.seek FlushPuck::Garage @19:00:54.28 Elbow.reconfigure LooseElbow @19:00:54.39 Forearm.seek :garage @19:00:55.42 Hand.close @19:00:56.32 Forearm.seek :retract @19:00:57.36 Elbow.reconfigure TightElbow @19:00:57.41 Elbow.seek CC @19:01:00.31 Elbow.reconfigure LooseElbow @19:01:00.42 Forearm.reconfigure TightForearm @19:01:00.47 Forearm.seek CC @19:01:01.77 CC.seek :closed @19:01:06.02 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.43 @19:01:06.10 Hand.open @19:01:06.89 Forearm.reconfigure LooseForearm @19:01:06.94 Forearm.seek :retract @19:01:07.90 AWV.select :air @19:01:08.34 AIV.dial :bypass @19:01:08.43 PSV.dial PSR @19:01:10.09 Pulling 1.5ml of PSR.flush @19:01:10.10 PSR.select :flush @19:01:10.23 PS.seek 1.5ml @19:01:26.39 delay 5 seconds @19:01:31.41 PSV.dial :waste1 @19:01:31.91 PS.seek 0ml @19:01:48.16 PSV.dial PSR @19:01:48.66 PSR.select :air @19:01:48.79 PS.seek 2.5ml @19:02:15.41 PRV.dial CBV @19:02:15.46
CBV.dial PRV @19:02:16.37 PSV.dial :mixloop @19:02:17.52 CTV.dial :air,avoiding: :intake @19:02:17.54 PTV.dial :PRVmixing @19:02:17.62 PS.seek 0ml @19:02:44.29 PSV.dial :air @19:02:44.81 PS.seek 2.5ml @19:03:11.45 PSV.dial :mixloop @19:03:11.97 PS.seek 0ml @19:03:38.41 PSV.dial :air @19:03:38.92 PS.seek 2.5ml @19:04:05.29 PSV.dial :mixloop @19:04:05.81 PS.seek 0ml @19:04:32.49 Garaging FlushPuck in Collection Clamp @19:04:32.57 Elbow.reconfigure TightElbow @19:04:32.62 Elbow.seek CC @19:04:32.86 Elbow.reconfigure LooseElbow @19:04:32.97 Forearm.seek CC @19:04:33.98 Hand.close @19:04:34.69 CC.seek :open @19:04:39.12 Forearm.seek :retract @19:04:40.12 Elbow.reconfigure TightElbow @19:04:40.17 Elbow.seek :garage @19:04:43.32 Elbow.reconfigure LooseElbow @19:04:43.43 Forearm.reconfigure TightForearm @19:04:43.48 Forearm.seek :garage @19:04:44.63 Hand.open @19:04:45.51 Forearm.reconfigure LooseForearm @19:04:45.56 Forearm.seek :retract @19:04:46.62 Connecting PS to CS to transfer sample @19:04:46.63 CBV.dial AIV @19:04:46.65 AIV.dial :bypass @19:04:46.66 PSV.dial :mixloop @19:04:46.67 PTV.dial :PRVmixing @19:04:46.69 PRV.dial AIV @19:04:46.70 CTV.dial :air,avoiding: :intake @19:04:46.72 Loading lyfil puck from tube 2 into Collection Clamp @19:04:46.97 SC.reconfigure SCtight @19:04:47.03 SC.seek 2 @19:04:49.31 SC.reconfigure SCloose @19:04:49.54 Elbow.reconfigure TightElbow @19:04:49.59 Elbow.seek 2 @19:04:51.61 Forearm.reconfigure TightForearm @19:04:51.66 Forearm.seek 2 + 700 counts @19:04:52.67 SE.reconfigure SEpick @19:04:52.75 SE.seek beamBreak @19:04:53.99 SE.configure SEconfig @19:04:54.16 Elbow.seek 2 @19:04:54.86 Forearm.seek 2 + 300 counts @19:04:55.11 Forearm.reconfigure LooseForearm @19:04:55.16 Hand.close @19:04:55.95 SE.seek :down @19:04:56.19
Forearm.seek :retract @19:04:56.87 SE.coast @19:04:57.18
Elbow.seek CC @19:04:58.90 Elbow.reconfigure LooseElbow @19:04:59.01 Forearm.reconfigure TightForearm @19:04:59.06 Forearm.seek CC @19:05:00.37 CC.seek :closed @19:05:04.62 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.38 @19:05:04.64 Hand.open @19:05:05.52 Forearm.reconfigure LooseForearm @19:05:05.57 Forearm.seek :retract @19:05:06.58 SC.coast @19:05:06.62 CTV.dial CSV,avoiding: :intake @19:05:08.16 CSV.dial CTV @19:05:08.52 CS.seek 5ml @19:05:22.46 delay 1:20 @19:06:42.50 CS.seek 2.5ml @19:06:49.93 delay 40 seconds @19:07:29.97 CS.seek 0ml @19:07:37.36 delay 1:00 @19:08:37.40 PSV.dial :air @19:08:37.91 PS.seek 2.5ml @19:09:04.35 PSV.dial :mixloop @19:09:04.86 delay 3 seconds @19:09:07.88 PSV.dial :air @19:09:08.39 delay 2 seconds @19:09:10.40 PSV.dial :mixloop @19:09:10.91 delay 3 seconds @19:09:13.93 PSV.dial :waste1 @19:09:15.47 PS.seek :empty @19:09:41.89 CBV.dial :waste1 @19:09:42.40 delay 5 seconds @19:09:47.43 CBV.dial AIV @19:09:47.96 delay 10 seconds @19:09:57.98 CBV.dial :waste1 @19:09:58.51 delay 10 seconds @19:10:08.53 CBV.dial AIV @19:10:09.06 delay 10 seconds @19:10:19.08 CTV.dial CSV,avoiding: :intake @19:10:19.10 CBV.dial between AIV and waste1 @19:10:19.48 CSV.dial :air @19:10:20.12 CS.seek 10ml @19:10:46.62 CSV.dial CTV @19:10:47.12 CS.seek 0ml @19:11:13.69 CBV.dial :waste1 @19:11:14.03 delay 10 seconds @19:11:24.06 CTV.dial :air,avoiding: :intake @19:11:25.63 Storing lyfil puck in Collection Clamp to tube 1 @19:11:25.82 SC.reconfigure SCtight @19:11:25.87 SC.seek 1 @19:11:28.01 SC.reconfigure SCloose @19:11:28.24 Elbow.reconfigure TightElbow @19:11:28.29 Elbow.seek 1 @19:11:30.16 Forearm.reconfigure TightForearm @19:11:30.21 Forearm.seek 1 + 700 counts @19:11:31.72 SE.reconfigure SEpick @19:11:31.88 SE.seek beamBreak @19:11:35.01 SE.configure SEconfig @19:11:35.19 Elbow.reconfigure LimpElbow @19:11:35.25 Forearm.reconfigure LooseForearm @19:11:35.29 Forearm.seek 1 @19:11:35.63 Elbow.hold @19:11:35.71 Elbow.reconfigure TightElbow @19:11:35.82 SE.seek 0.06 Pucks below Top Plate @19:11:36.27 Forearm.force -20 @19:11:36.31 Forearm.seek 1 + 319 counts @19:11:36.64 Forearm.reconfigure TightForearm @19:11:36.69 Forearm.seek 1 + 700 counts @19:11:37.00 SE.seek 1.06 Pucks below Top Plate @19:11:37.62 Forearm.reconfigure LooseForearm @19:11:37.67 Forearm.seek :retract @19:11:38.62 Elbow.seek CC @19:11:40.32 Elbow.reconfigure LooseElbow @19:11:40.42 Forearm.seek CC @19:11:41.41 Hand.close @19:11:42.20 CC.seek :open @19:11:46.73 Forearm.seek :retract @19:11:47.75 Elbow.reconfigure TightElbow @19:11:47.80 Elbow.seek 1 @19:11:49.73 Elbow.reconfigure LimpElbow @19:11:49.79 Forearm.seek 1 @19:11:50.82 Elbow.hold @19:11:50.90 Elbow.reconfigure TightElbow @19:11:50.95 Hand.open @19:11:51.84 Forearm.force -20 @19:11:51.88 Forearm.seek 1 + 340 counts @19:11:52.19 Forearm.reconfigure TightForearm @19:11:52.24 Forearm.seek 1 + 700 counts @19:11:52.56 SE.seek :down @19:11:53.70
Forearm.reconfigure LooseForearm @19:11:53.75 Forearm.seek :retract @19:11:55.30 SE.coast @19:11:55.34
SC.coast @19:11:55.38 PSV.dial :air @19:11:56.73 PS.seek 0.499ml @19:12:02.76 delay 3 seconds @19:12:05.78 PSV.dial :mixloop @19:12:06.29 PRV.dial :air @19:12:06.80 delay 3 seconds @19:12:09.82 PRV.dial CBV @19:12:10.32 CBV.dial AIV @19:12:10.84 PRV.dial between CBV and air @19:12:11.21 PRV.dial :air @19:12:11.61 PS.reconfigure PSslow @19:12:11.66 PS.seek 0.599ml @19:12:14.17 PRV.dial :waste2 @19:12:15.01 PS.reconfigure PSconfig @19:12:15.06 PS.seek :empty @19:12:22.02 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:12:22.09 Hand.open @19:12:22.96 Elbow.seek FlushPuck::Garage @19:12:24.84 Elbow.reconfigure LooseElbow @19:12:24.95 Forearm.seek :garage @19:12:26.01 Hand.close @19:12:26.84 Forearm.seek :retract @19:12:27.89 Elbow.reconfigure TightElbow @19:12:27.93 Elbow.seek CC @19:12:30.79 Elbow.reconfigure LooseElbow @19:12:30.90 Forearm.reconfigure TightForearm @19:12:30.95 Forearm.seek CC @19:12:32.27 CC.seek :closed @19:12:36.54 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.37 @19:12:36.61 Hand.open @19:12:37.39 Forearm.reconfigure LooseForearm @19:12:37.44 Forearm.seek :retract @19:12:38.48 Clearing PML for modified lysate @19:12:38.50 CTV.dial :air,avoiding: :intake @19:12:38.51 PRV.dial :waste2 @19:12:38.52 PTV.dial :PRV,avoiding: :puck @19:12:39.35 delay 2 seconds @19:12:41.36 PTV.dial :PRVmixing,avoiding: :puck @19:12:42.21 delay 2 seconds @19:12:44.22 PSV.dial :PML @19:12:44.24 delay 2 seconds @19:12:46.26 PRV.dial :air @19:12:47.11 delay 2 seconds @19:12:49.21 PS.seek 1.499ml @19:13:05.35 delay 5 seconds @19:13:10.38 PS.seek 2.5ml @19:13:21.48 PSV.dial :air @19:13:22.00 PS.seek 0ml @19:13:48.35 mixing lysate with speDil @19:13:48.37 PSV.dial :PML @19:13:48.87 CBV.dial :PRV @19:13:48.89 PRV.dial :air @19:13:48.90 delay 2 seconds @19:13:50.92 PRV.dial :speDil @19:13:51.46 delay 2 seconds @19:13:53.55 PS.reconfigure PSslow1 @19:13:53.60 PS.seek 0.225ml @19:14:17.70 delay 5 seconds @19:14:22.73 PRV.dial CBV @19:14:23.60 delay 2 seconds @19:14:25.68 PS.seek 0.475ml @19:14:51.42 delay 5 seconds @19:14:56.45 PRV.dial :air @19:14:56.97 delay 2 seconds @19:14:58.99 PS.seek 0.965ml @19:15:49.10 delay 10 seconds @19:15:59.13 PRV.dial CBV @19:15:59.64 CTV.dial :air,avoiding: :intake @19:15:59.66 PSV.dial :air @19:16:00.18 PS.reconfigure PSconfig @19:16:00.23 PS.seek 2.5ml @19:16:16.54 delay 3 seconds @19:16:19.56 PSV.dial PTV @19:16:20.40 PTV.dial :PRV,avoiding: :puck @19:16:21.24 PS.seek 0ml @19:16:47.95 PSV.dial :air @19:16:48.83 PS.seek 2.5ml @19:17:15.07 delay 3 seconds @19:17:18.09 PSV.dial PTV @19:17:19.05 PTV.dial :PRV,avoiding: :puck @19:17:19.07 PS.seek 0ml @19:17:45.29 Pushing modified lysate into AIV Injection Loop @19:17:45.30 CBV.dial AIV @19:17:45.32 AIV.dial :bypass @19:17:45.33 PRV.dial CBV @19:17:45.34 PSV.dial :air @19:17:46.16 PS.seek 2.5ml @19:18:12.42 delay 3 seconds @19:18:15.44 PSV.dial :PML @19:18:15.94 PTV.dial :PRVmixing,avoiding: :puck @19:18:16.84 PS.reconfigure PSslow1 @19:18:16.89 PS.seek 1.74ml @19:19:34.76 delay 5 seconds @19:19:39.79 AIV.dial :sample @19:19:40.41 AWV.select :waste @19:19:40.59 PS.reconfigure PSslow2 @19:19:40.64 PS.seek 1.22ml @19:21:28.92 delay 15 seconds @19:21:43.95 AIV.dial :bypass @19:21:44.59 PS.reconfigure PSconfig @19:21:44.64 PS.seek 0ml @19:21:57.92 PSV.dial :air @19:21:58.43 PS.seek 2.5ml @19:22:24.79 PSV.dial :PML @19:22:25.29 PS.seek 0ml @19:22:51.51 AIV.dial :coilToMFB @19:22:51.52 AWV.select :air @19:22:51.69 Extracting Nucleic Acid from AIV @19:22:51.72
Beginning samplePassD @19:22:51.75 SPE.hold 2:46:40,55C @19:22:51.86 ASV.dial :SPEdelivery @19:22:51.93
AWV.select :air @19:22:51.94 AIV.dial :bypass @19:22:51.96 PSV.dial PSR @19:22:52.26 delay 20 seconds @19:22:53.25
Pulling 1.5ml of PSR.killMFB @19:22:53.26 PSR.select :killMFB @19:22:53.39 PS.seek 1.5ml @19:23:10.27 delay 5 seconds @19:23:12.30 primeLysateINT @19:23:12.31 AWV.select :air @19:23:12.32 AIV.dial :coilToMFB @19:23:12.33 ATV.dial AIV @19:23:14.20 AS.reconfigure ASslow1 @19:23:14.25 AS.seek 0.299ml @19:23:15.29
PSV.dial :waste1 @19:23:15.84 PS.seek 0ml @19:23:32.95 PSV.dial PSR @19:23:33.48 PSR.select :air @19:23:33.61 PS.seek 2.5ml @19:24:01.42 PRV.dial CBV @19:24:01.45
CBV.dial PRV @19:24:01.47 PSV.dial :mixloop @19:24:02.69 CTV.dial :air,avoiding: :intake @19:24:02.70 PTV.dial :PRVmixing @19:24:02.72 PS.seek 0ml @19:24:14.90 delay 10 seconds @19:24:24.93 ATV.dial :air2 @19:24:25.82 AS.seek 0.329ml @19:24:30.71
PSV.dial PSR @19:24:32.00 Pulling 1.5ml of PSR.flush @19:24:32.01 PSR.select :flush @19:24:32.13 ATV.dial :waste2 @19:24:32.19
PS.seek 1.5ml @19:24:32.81 AS.reconfigure ASnorm @19:24:32.86 AS.seek :empty @19:24:49.21
delay 5 seconds @19:24:49.82 Lysate primed! @19:24:49.84 AFV.select :holdingCoil @19:24:49.85 ATV.dial :air2 @19:24:50.50 AS.reconfigure ASslow1 @19:24:50.56 AS.seek 0.069ml @19:24:54.23
PSV.dial :waste1 @19:24:54.76 PS.seek 0ml @19:25:05.04 delay 3 seconds @19:25:08.07 ATV.dial :AIV @19:25:08.96 AS.seek 0.469ml @19:25:12.14
PSV.dial PSR @19:25:12.67 PSR.select :air @19:25:12.80 PS.seek 2.5ml @19:25:40.46 PRV.dial CBV @19:25:40.49
CBV.dial PRV @19:25:40.51 PSV.dial :mixloop @19:25:41.82 CTV.dial :air,avoiding: :intake @19:25:41.83 PTV.dial :PRVmixing @19:25:41.85 PS.seek 0ml @19:26:09.20 PSV.dial PSR @19:26:10.44 Pulling 1.5ml of PSR.flush @19:26:10.45 PSR.select :flush @19:26:10.58 PS.seek 1.5ml @19:26:27.18 delay 5 seconds @19:26:29.66 delay 5 seconds @19:26:32.20
PSV.dial :waste1 @19:26:32.73 PS.seek 0ml @19:26:34.75 Solid Phase Extraction Heater at 50.4C @19:26:34.76 ATV.dial ASV @19:26:35.15 ASV.dial :SPEdelivery @19:26:35.24 AS.seek 0.041ml @19:26:49.39
PSV.dial PSR @19:26:49.92 PSR.select :air @19:26:50.05 PS.seek 2.5ml @19:27:17.02 PRV.dial CBV @19:27:17.05
CBV.dial PRV @19:27:17.06 PSV.dial :mixloop @19:27:18.37 CTV.dial :air,avoiding: :intake @19:27:18.38 PTV.dial :PRVmixing @19:27:18.40 PS.seek 0ml @19:27:45.58 PSV.dial PSR @19:27:46.80 Pulling 1.5ml of PSR.flush @19:27:46.81 PSR.select :flush @19:27:46.94 PS.seek 1.5ml @19:28:01.62 delay 10 seconds @19:28:03.37
delay 5 seconds @19:28:08.39 PSV.dial :waste1 @19:28:08.91 PS.seek 0ml @19:28:11.65 rinseLysateLineINT @19:28:11.66 AFV.select :carrierSolution @19:28:11.77 delay 2 seconds @19:28:13.80 AS.reconfigure ASconfig @19:28:13.85 AS.seek 1ml @19:28:25.33
PSV.dial PSR @19:28:25.97 PSR.select :air @19:28:26.10 PS.seek 2.5ml @19:28:52.84 PRV.dial CBV @19:28:52.86
CBV.dial PRV @19:28:52.88 PSV.dial :mixloop @19:28:54.07 CTV.dial :air,avoiding: :intake @19:28:54.08 PTV.dial :PRVmixing @19:28:54.10 PS.reconfigure PSslow @19:28:54.15 PS.seek 1.2ml @19:29:09.30 delay 1 second @19:29:10.32 AFV.select :holdingCoil @19:29:10.43 delay 1 second @19:29:11.45 ATV.dial AIV @19:29:12.02 AIV.dial :coilToMFB @19:29:12.03 AWV.select :waste @19:29:12.15 AS.reconfigure ASslow0 @19:29:12.20 AS.seek 0ml @19:29:21.02
PSV.dial :waste1 @19:29:22.53 PS.reconfigure PSconfig @19:29:22.58 PS.seek 0ml @19:29:35.80 PSV.dial :air @19:29:36.98 PS.seek 2.5ml @19:30:03.42 PSV.dial :mixloop @19:30:03.92 PS.reconfigure PSslow @19:30:03.97 PS.seek 0ml @19:30:53.10 ATV.dial :air2 @19:30:53.76 AS.reconfigure ASslow1 @19:30:53.81 AS.seek 0.05ml @19:30:55.02
PSV.dial :air @19:30:55.54 PS.reconfigure PSconfig @19:30:55.59 PS.seek 2.5ml @19:31:04.15 ATV.dial :AIV @19:31:05.49 AS.seek 0.01ml @19:31:13.82 ATV.dial :waste @19:31:15.47 AS.seek :empty @19:31:18.05 ethanol wash @19:31:18.07 ATV.dial :air2 @19:31:19.34 AS.reconfigure ASslow2 @19:31:19.40 AS.seek 0.009ml @19:31:22.08
PSV.dial :mixloop @19:31:22.60 PS.reconfigure PSslow @19:31:22.65 PS.seek 0ml @19:31:29.68 delay 3 seconds @19:31:32.72 Pulling 0.4ml of Ethanol Wash @19:31:32.73 ATV.dial :ethanol @19:31:33.56 AS.reconfigure ASslow1 @19:31:33.61 AS.seek 0.409ml @19:32:13.80
Cleaning Collection Syringe @19:32:13.81 CSV.dial CSR @19:32:14.38 Pulling 4ml of CSR.flush @19:32:14.39 CSR.select :flush @19:32:14.52 CS.seek 4ml @19:32:25.71 delay 5 seconds @19:32:30.74 CSR.select :air @19:32:30.86 CS.seek 10ml @19:32:47.21 CSV.dial CTV @19:32:47.24
CTV.dial CSV @19:32:48.79 CBV.dial :waste1 @19:32:49.34 CS.seek 0ml @19:32:54.42 delay 3 seconds @19:32:57.44 ATV.dial :air1 @19:32:57.85 delay 3 seconds @19:33:00.93 AS.seek 0.434ml @19:33:06.33 delay 3 seconds @19:33:09.35 ATV.dial :ethanol @19:33:09.98 AS.seek 0.419ml @19:33:13.19 delay 3 seconds @19:33:15.79
CSV.dial CSR @19:33:16.21 ATV.dial ASV @19:33:16.36
Pulling 4ml of CSR.flush @19:33:16.37 CSR.select :flush @19:33:16.50 CS.seek 4ml @19:33:17.57 AS.seek 0.092ml @19:33:27.65
delay 5 seconds @19:33:32.67 CSR.select :air @19:33:32.80 CS.seek 10ml @19:33:49.21 CSV.dial CTV @19:33:49.24
CTV.dial CSV @19:33:49.76 CBV.dial :waste1 @19:33:49.78 CS.seek 0ml @19:34:16.43 Garaging FlushPuck in Collection Clamp @19:34:16.51 Elbow.reconfigure TightElbow @19:34:16.56 Elbow.seek CC @19:34:16.79 Elbow.reconfigure LooseElbow @19:34:16.90 Forearm.seek CC @19:34:17.89 Hand.close @19:34:18.60 CC.seek :open @19:34:23.00 Forearm.seek :retract @19:34:23.59 delay 3 seconds @19:34:23.99
Elbow.reconfigure TightElbow @19:34:24.04 Elbow.seek :garage @19:34:26.61 ATV.dial :waste2 @19:34:27.21
Elbow.reconfigure LooseElbow @19:34:27.32 Forearm.reconfigure TightForearm @19:34:27.40 Forearm.seek :garage @19:34:27.43 AS.reconfigure ASconfig @19:34:27.53 AS.seek :empty @19:34:28.63
Hand.open @19:34:29.42 Forearm.reconfigure LooseForearm @19:34:29.47 Forearm.seek :retract @19:34:30.54 Whole Cell Archive sampling 200ml @19:34:30.55 Loading wcr puck from tube 2 into Collection Clamp @19:34:31.34 SC.reconfigure SCtight @19:34:31.40 SC.seek 2 @19:34:33.18 ATV.dial :air2 @19:34:33.59 AS.reconfigure ASslow1 @19:34:33.67 AS.seek 1ml @19:34:33.70 SC.reconfigure SCloose @19:34:34.00 Elbow.reconfigure TightElbow @19:34:34.05 Elbow.seek 2 @19:34:36.21 Forearm.reconfigure TightForearm @19:34:36.26 Forearm.seek 2 + 700 counts @19:34:37.38 SE.reconfigure SEpick @19:34:37.45 SE.seek beamBreak @19:34:38.80 SE.configure SEconfig @19:34:38.98 Elbow.seek 2 @19:34:39.21 Forearm.seek 2 + 300 counts @19:34:39.48 Forearm.reconfigure LooseForearm @19:34:39.53 Hand.close @19:34:40.24 SE.seek :down @19:34:40.49 Forearm.seek :retract @19:34:41.34 SE.coast @19:34:41.43 Elbow.seek CC @19:34:43.10 Elbow.reconfigure LooseElbow @19:34:43.22 Forearm.reconfigure TightForearm @19:34:43.27 Forearm.seek CC @19:34:44.60 CC.seek :closed @19:34:48.81 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.47 @19:34:48.83 Hand.open @19:34:49.67 Forearm.reconfigure LooseForearm @19:34:49.72 Forearm.seek :retract @19:34:50.73 SC.coast @19:34:50.78 collectSample 200ml w/bubble point of 55psi @19:34:50.79 Priming 7ml @19:34:50.80 Intake.select :close @19:34:50.81 Exhaust.select :close @19:34:50.83 SS.hold @19:34:51.06 Calibrating pressure sensors @19:34:51.07 CTV.dial :air @19:34:52.58 SSV.dial :spit,avoiding: :exhaust @19:34:52.61 SS.reconfigure SSfast @19:34:52.66 SS.seek 5ml @19:34:55.41 SSV.dial CBV @19:34:55.44 CBV.dial SSV @19:34:57.04 delay 3.8 seconds @19:35:00.99 SSV.dial :spit @19:35:02.49 SS.seek 0ml @19:35:05.29 SSV.dial CBV @19:35:06.79 CTV.dial :intake @19:35:08.16 SSV.dial CBV @19:35:08.19 CBV.dial SSV @19:35:08.21 Allowing 40s for 7.0ml of remaining 7.0ml @19:35:08.22 Intake.select :open @19:35:10.40 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:35:10.43 SS.reconfigure SSinhale.with maxSpeed: 150 @19:35:10.48 SS.seek 7ml @19:35:16.98 Stroke completed -- waiting for pressure to equalize ... @19:35:41.12 Intake.select :close @19:35:41.27 SSV.dial :exhaust @19:35:42.16 Exhaust.select :open @19:35:42.28 SS.reconfigure SSexhale @19:35:42.33 SS.seek 0ml @19:35:46.66 Exhaust.select :close @19:35:46.78 Sampling 200.0ml @19:35:46.79 Intake.select :close @19:35:46.80 Exhaust.select :close @19:35:46.82 SS.reconfigure SSconfig @19:35:46.86 SS.hold @19:35:47.09 CTV.dial :intake @19:35:47.11 SSV.dial CBV @19:35:47.14 CBV.dial SSV @19:35:48.03 Allowing 130s for 25.0ml of remaining 200.0ml @19:35:48.04 Intake.select :open @19:35:50.22 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:35:50.24 SS.reconfigure SSinhale.with maxSpeed: 150 @19:35:50.29 SS.seek 25ml @19:35:54.85 Vacuum too high -- waiting up to 40s @19:36:05.96 Intake @16.2psia, vacuum from 9.7 to 13.7psi @19:36:05.98 SS.reconfigure SSinhale.with maxSpeed: 135 @19:36:06.03 SS.seek 25ml @19:36:09.73 Vacuum too high -- waiting up to 40s @19:36:19.72 Intake @16.1psia, vacuum from 9.6 to 13.6psi @19:36:19.74 SS.reconfigure SSinhale.with maxSpeed: 122 @19:36:19.79 SS.seek 25ml @19:36:24.73 Vacuum too high -- waiting up to 40s @19:36:36.43 Intake @16.0psia, vacuum from 9.5 to 13.5psi @19:36:36.45 SS.reconfigure SSinhale.with maxSpeed: 110 @19:36:36.51 SS.seek 25ml @19:36:41.20 Vacuum too high -- waiting up to 40s @19:36:52.11 Intake @16.0psia, vacuum from 9.5 to 13.5psi @19:36:52.13 SS.reconfigure SSinhale.with maxSpeed: 99 @19:36:52.18 SS.seek 25ml @19:36:52.77 Stroke completed -- waiting for pressure to equalize ... @19:36:58.46 Intake.select :close @19:36:58.62 SSV.dial :exhaust @19:36:59.49 Exhaust.select :open @19:36:59.61 SS.reconfigure SSexhale @19:36:59.66 SS.seek 0ml @19:37:09.20 Exhaust.select :close @19:37:09.32 SSV.dial CBV @19:37:09.35 CBV.dial SSV @19:37:10.23 Allowing 130s for 25.0ml of remaining 175.0ml @19:37:10.24 Intake.select :open @19:37:12.42 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:37:12.44 SS.reconfigure SSinhale.with maxSpeed: 99 @19:37:12.49 SS.seek 25ml @19:37:16.64 Vacuum too high -- waiting up to 40s @19:37:22.38 Intake @15.8psia, vacuum from 9.3 to 13.3psi @19:37:22.40 SS.reconfigure SSinhale.with maxSpeed: 89 @19:37:22.45 SS.seek 25ml @19:37:26.42 Vacuum too high -- waiting up to 40s @19:37:32.98 Intake @16.0psia, vacuum from 9.5 to 13.5psi @19:37:33.00 SS.reconfigure SSinhale.with maxSpeed: 80 @19:37:33.05 SS.seek 25ml @19:37:36.99 Vacuum too high -- waiting up to 40s @19:37:42.54 Intake @15.9psia, vacuum from 9.4 to 13.4psi @19:37:42.57 SS.reconfigure SSinhale.with maxSpeed: 72 @19:37:42.62 SS.seek 25ml @19:37:46.79 Vacuum too high -- waiting up to 40s @19:37:52.54 SS.reconfigure SSinhale.with maxSpeed: 65 @19:37:52.60 SS.seek 25ml @19:37:55.41 delay 5 seconds @19:37:58.39 Vacuum too high -- waiting up to 40s @19:38:00.77 ATV.dial ASV @19:38:01.81 ASV.dial :SPEdelivery @19:38:01.84 AS.seek 0.02ml @19:38:04.72 Intake @15.8psia, vacuum from 9.3 to 13.3psi @19:38:04.74 SS.reconfigure SSinhale.with maxSpeed: 59 @19:38:04.79 SS.seek 25ml @19:38:10.26 Stroke completed -- waiting for pressure to equalize ... @19:38:19.22 Intake.select :close @19:38:19.39 SSV.dial :exhaust @19:38:20.24 Exhaust.select :open @19:38:20.36 SS.reconfigure SSexhale @19:38:20.42 SS.seek 0ml @19:38:29.66 Exhaust.select :close @19:38:29.78 SSV.dial CBV @19:38:29.81 CBV.dial SSV @19:38:30.70 Allowing 130s for 25.0ml of remaining 150.0ml @19:38:30.71 Intake.select :open @19:38:32.89 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:38:32.91 SS.reconfigure SSinhale.with maxSpeed: 59 @19:38:32.96 SS.seek 25ml @19:38:39.98 Vacuum too high -- waiting up to 40s @19:38:45.01 Intake @15.7psia, vacuum from 9.2 to 13.2psi @19:38:45.03 SS.reconfigure SSinhale.with maxSpeed: 53 @19:38:45.08 SS.seek 25ml @19:38:52.13 Vacuum too high -- waiting up to 40s @19:38:57.66 SS.reconfigure SSinhale.with maxSpeed: 48 @19:38:57.72 SS.seek 25ml @19:39:09.61 Vacuum too high -- waiting up to 40s @19:39:16.89 Intake @15.8psia, vacuum from 9.3 to 13.3psi @19:39:16.91 SS.reconfigure SSinhale.with maxSpeed: 43 @19:39:16.96 SS.seek 25ml @19:39:28.97 Stroke completed -- waiting for pressure to equalize ... @19:39:39.08 Intake.select :close @19:39:39.24 SSV.dial :exhaust @19:39:40.11 Exhaust.select :open @19:39:40.23 SS.reconfigure SSexhale @19:39:40.28 SS.seek 0ml @19:39:49.85 Exhaust.select :close @19:39:49.97 SSV.dial CBV @19:39:50.00 CBV.dial SSV @19:39:50.89 Allowing 130s for 25.0ml of remaining 125.0ml @19:39:50.90 Intake.select :open @19:39:53.08 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:39:53.10 SS.reconfigure SSinhale.with maxSpeed: 43 @19:39:53.15 SS.seek 25ml @19:40:07.76 Vacuum too high -- waiting up to 40s @19:40:13.53 SS.reconfigure SSinhale.with maxSpeed: 39 @19:40:13.59 SS.seek 25ml @19:40:32.45 Vacuum too high -- waiting up to 40s @19:40:38.54 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:40:38.56 SS.reconfigure SSinhale.with maxSpeed: 35 @19:40:38.62 SS.seek 25ml @19:40:51.32 Stroke completed -- waiting for pressure to equalize ... @19:40:59.01 Intake.select :close @19:40:59.17 SSV.dial :exhaust @19:41:00.02 Exhaust.select :open @19:41:00.14 SS.reconfigure SSexhale @19:41:00.19 SS.seek 0ml @19:41:09.89 Exhaust.select :close @19:41:10.01 SSV.dial CBV @19:41:10.04 CBV.dial SSV @19:41:10.91 Allowing 130s for 25.0ml of remaining 100.0ml @19:41:10.92 Intake.select :open @19:41:13.10 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:41:13.12 SS.reconfigure SSinhale.with maxSpeed: 35 @19:41:13.17 SS.seek 25ml @19:41:19.19 delay 5 seconds @19:41:24.21 ATV.dial :air2 @19:41:25.29 AS.seek 0.52ml @19:41:52.38 Vacuum too high -- waiting up to 40s @19:41:58.94 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:41:58.97 SS.reconfigure SSinhale.with maxSpeed: 32 @19:41:59.02 SS.seek 25ml @19:42:11.43 Stroke completed -- waiting for pressure to equalize ... @19:42:18.50 Intake.select :close @19:42:18.66 SSV.dial :exhaust @19:42:19.54 Exhaust.select :open @19:42:19.66 SS.reconfigure SSexhale @19:42:19.71 SS.seek 0ml @19:42:29.43 Exhaust.select :close @19:42:29.55 SSV.dial CBV @19:42:29.58 CBV.dial SSV @19:42:30.47 Allowing 130s for 25.0ml of remaining 75.0ml @19:42:30.48 Intake.select :open @19:42:32.66 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:42:32.68 SS.reconfigure SSinhale.with maxSpeed: 32 @19:42:32.74 SS.seek 25ml @19:43:06.23 delay 5 seconds @19:43:11.26 ATV.dial ASV @19:43:12.26 ASV.dial :wasteR @19:43:12.66 AS.seek 0.3ml @19:43:27.74 Stroke completed -- waiting for pressure to equalize ... @19:43:37.50 Intake.select :close @19:43:37.65 SSV.dial :exhaust @19:43:38.54 Exhaust.select :open @19:43:38.66 SS.reconfigure SSexhale @19:43:38.71 SS.seek 0ml @19:43:47.96 Exhaust.select :close @19:43:48.08 SSV.dial CBV @19:43:48.11 CBV.dial SSV @19:43:49.00 Allowing 130s for 25.0ml of remaining 50.0ml @19:43:49.01 Intake.select :open @19:43:51.19 Intake @15.7psia, vacuum from 9.2 to 13.2psi @19:43:51.21 SS.reconfigure SSinhale.with maxSpeed: 32 @19:43:51.26 SS.seek 25ml @19:43:57.14 delay 20 seconds @19:44:17.17 AS.seek 0.07ml @19:44:46.99 Stroke completed -- waiting for pressure to equalize ... @19:44:57.11 Intake.select :close @19:44:57.31 SSV.dial :exhaust @19:44:58.16 Exhaust.select :open @19:44:58.28 SS.reconfigure SSexhale @19:44:58.33 SS.seek 0ml @19:45:03.68 delay 20 seconds @19:45:07.92 Exhaust.select :close @19:45:08.04 SSV.dial CBV @19:45:08.09 CBV.dial SSV @19:45:08.93 Allowing 130s for 25.0ml of remaining 25.0ml @19:45:08.94 Intake.select :open @19:45:11.12 Intake @15.7psia, vacuum from 9.2 to 13.2psi @19:45:11.14 SS.reconfigure SSinhale.with maxSpeed: 32 @19:45:11.19 SS.seek 24.99ml @19:45:23.70 ATV.dial :waste2 @19:45:24.58 AS.reconfigure ASconfig @19:45:24.63 AS.seek :empty @19:45:28.99 Elution @19:45:29.01 ATV.dial :air1 @19:45:30.16 AS.reconfigure ASslow1 @19:45:30.21 AS.seek 0.049ml @19:45:40.62 delay 3 seconds @19:45:43.65 ATV.dial :ElutionWater @19:45:44.35 AS.seek 0.109ml @19:45:56.68 delay 3 seconds @19:45:59.71 ATV.dial ASV,via: :air2 @19:46:01.83 ASV.dial :SPEelution @19:46:02.43 AFV.select :carrierSolution @19:46:02.60 AS.reconfigure ASnorm @19:46:02.65 AS.seek 0.759ml @19:46:06.28 Stroke completed -- waiting for pressure to equalize ... @19:46:17.77 Intake.select :close @19:46:17.92 SSV.dial :exhaust @19:46:18.80 Exhaust.select :open @19:46:18.92 SS.reconfigure SSexhale @19:46:18.97 SS.seek 0ml @19:46:28.28 Exhaust.select :close @19:46:28.40 Sampled 200.0ml @19:46:28.42 Drying puck before fixing for 4:00 @19:46:28.43 CBV.dial :waste2b @19:46:29.28 delay 1:00 @19:46:35.77 delay 3 seconds @19:46:38.80 AFV.select :holdingCoil @19:46:38.96 AS.reconfigure ASslow1 @19:46:39.01 AS.seek 0.474ml @19:47:29.30 CSV.dial :air @19:47:29.81 CS.reconfigure CSfast @19:47:29.87 CS.seek 10ml @19:47:37.27 delay 5 seconds @19:47:42.38 AS.reconfigure ASslow2 @19:47:42.43 AS.seek 0.439ml @19:47:56.34 Pumping wcr puck from tube 2 up to 55psi... @19:47:56.35 CBV.dial SSV @19:47:57.78 CBV.dial between CSV and waste2b @19:47:58.90 CSV.dial between air and CTV @19:47:59.27 CS.seek 7.266ml @19:48:07.31 CTV.dial CSV,avoiding: :air @19:48:07.34 CSV.dial CTV @19:48:08.23 CS.reconfigure CSpressurize @19:48:08.29 CS.seek 0ml @19:48:17.95 delay 1:00 @19:48:24.04 CBV.dial :waste2b @19:48:24.42 CS.reconfigure CSconfig @19:48:24.47 CS.seek :empty @19:48:29.02 delay 1:00 @19:49:17.98 pushing eluate to holding coil @19:49:18.05 AS.seek 0.279ml @19:49:29.06 CTV.dial :air,avoiding: :intake @19:49:30.58 Fix #1 of 2 (with rnal) for 2:00 @19:49:30.60 CSV.dial CSR @19:49:31.15 Pulling 2ml of CSR.rnal @19:49:31.17 CSR.select :rnal @19:49:31.29 CS.seek 2ml @19:49:37.50 delay 5 seconds @19:49:42.52 CSR.select :air @19:49:42.65 CS.reconfigure CSfast @19:49:42.70 CS.seek 10ml @19:50:04.06 Pumping wcr puck from tube 2 up to 55psi... @19:50:04.07 CBV.dial SSV @19:50:05.48 CBV.dial between CSV and waste2b @19:50:06.57 CSV.dial between air and CTV @19:50:07.38 CS.seek 7.189ml @19:50:15.50 CTV.dial CSV,avoiding: :air @19:50:15.53 CSV.dial CTV @19:50:17.09 CS.reconfigure CSpressurize @19:50:17.15 CS.seek 0ml @19:50:34.93 delay 2:00 @19:51:59.27 delay 30 seconds @19:52:29.30 ATV.dial :waste,via: :air3 @19:52:31.11 AS.reconfigure ASconfig @19:52:31.16 AS.seek :empty @19:52:34.95 CBV.dial :waste2b @19:52:35.33 CS.reconfigure CSconfig @19:52:35.38 CS.seek :empty @19:52:37.63 delay 1:00 @19:52:47.57 Parking Bleach in LysateSource line and SPEcolumn @19:52:47.59 ATV.dial :air3 @19:52:48.41 AS.reconfigure ASslow1 @19:52:48.46 AS.seek 0.029ml @19:52:54.74 ATV.dial :ElutionWater @19:52:55.37 AS.seek 0.059ml @19:53:01.68 ATV.dial :air3 @19:53:02.32 AS.seek 0.069ml @19:53:04.60 delay 2 seconds @19:53:06.62 ATV.dial :bleach @19:53:07.09 AS.seek 0.769ml @19:53:37.65 CTV.dial :air,avoiding: :intake @19:53:39.16 Fix #2 of 2 (with rnal) for 2:00 @19:53:39.18 CSV.dial CSR @19:53:39.73 Pulling 2ml of CSR.rnal @19:53:39.74 CSR.select :rnal @19:53:39.87 CS.seek 2ml @19:53:46.21 delay 5 seconds @19:53:51.24 CSR.select :air @19:53:51.37 CS.reconfigure CSfast @19:53:51.42 CS.seek 10ml @19:54:12.72 Pumping wcr puck from tube 2 up to 55psi... @19:54:12.73 CBV.dial SSV @19:54:14.14 CBV.dial between CSV and waste2b @19:54:15.24 CSV.dial between air and CTV @19:54:15.95 CS.seek 7.172ml @19:54:24.16 CTV.dial CSV,avoiding: :air @19:54:24.20 CSV.dial CTV @19:54:25.75 CS.reconfigure CSpressurize @19:54:25.81 CS.seek 0ml @19:54:42.90 delay 2:00 @19:55:28.68 delay 3 seconds @19:55:31.71 ATV.dial :air3 @19:55:32.26 delay 3 seconds @19:55:35.36 AS.seek 0.794ml @19:55:40.72 delay 3 seconds @19:55:43.75 ATV.dial :bleach @19:55:44.38 AS.seek 0.779ml @19:55:47.66 delay 3 seconds @19:55:50.69 ATV.dial :AIV @19:55:51.94 AS.reconfigure ASconfig @19:55:51.99 AS.seek 0.015ml @19:56:36.19 ATV.dial :waste @19:56:37.66 AS.seek :empty @19:56:38.85 ATV.dial :air3 @19:56:39.54 AS.reconfigure ASslow1 @19:56:39.59 AS.seek 0.009ml @19:56:42.07 delay 2 seconds @19:56:42.92 CBV.dial :waste2b @19:56:43.30 CS.reconfigure CSconfig @19:56:43.35 CS.seek :empty @19:56:44.09 ATV.dial :bleach @19:56:44.55 AS.seek 0.579ml @19:56:46.19 delay 1:00 @19:57:46.21 CTV.dial :air,avoiding: :intake @19:57:47.78 Storing wcr puck in Collection Clamp to tube 1 @19:57:47.98 SC.reconfigure SCtight @19:57:48.03 SC.seek 1 @19:57:50.19 SC.reconfigure SCloose @19:57:50.44 Elbow.reconfigure TightElbow @19:57:50.49 Elbow.seek 1 @19:57:52.41 Forearm.reconfigure TightForearm @19:57:52.46 Forearm.seek 1 + 700 counts @19:57:53.54 SE.reconfigure SEpick @19:57:53.61 SE.seek beamBreak @19:57:56.76 SE.configure SEconfig @19:57:56.94 Elbow.reconfigure LimpElbow @19:57:56.99 Forearm.reconfigure LooseForearm @19:57:57.04 Forearm.seek 1 @19:57:57.90 Elbow.hold @19:57:58.00 Elbow.reconfigure TightElbow @19:57:58.12 SE.seek 1.07 Pucks below Top Plate @19:57:58.60 Forearm.force -20 @19:57:58.65 Forearm.seek 1 + 316 counts @19:57:58.99 Forearm.reconfigure TightForearm @19:57:59.05 Forearm.seek 1 + 700 counts @19:57:59.33 SE.seek 2.07 Pucks below Top Plate @19:57:59.95 Forearm.reconfigure LooseForearm @19:58:00.01 Forearm.seek :retract @19:58:00.97 Elbow.seek CC @19:58:02.73 Elbow.reconfigure LooseElbow @19:58:02.85 Forearm.seek CC @19:58:03.81 Hand.close @19:58:04.63 CC.seek :open @19:58:09.15 Forearm.seek :retract @19:58:10.18 Elbow.reconfigure TightElbow @19:58:10.23 Elbow.seek 1 @19:58:12.23 Elbow.reconfigure LimpElbow @19:58:12.29 Forearm.seek 1 @19:58:13.33 Elbow.hold @19:58:13.40 Elbow.reconfigure TightElbow @19:58:13.46 Hand.open @19:58:14.39 Forearm.force -20 @19:58:14.43 Forearm.seek 1 + 349 counts @19:58:14.77 Forearm.reconfigure TightForearm @19:58:14.83 Forearm.seek 1 + 700 counts @19:58:15.15 SE.seek :down @19:58:16.31 Forearm.reconfigure LooseForearm @19:58:16.36 Forearm.seek :retract @19:58:17.79 SE.coast @19:58:17.83 SC.coast @19:58:17.87 Starting finalPurge @19:58:17.89 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:58:17.97 Hand.open @19:58:18.39 Elbow.seek FlushPuck::Garage @19:58:20.28 Elbow.reconfigure LooseElbow @19:58:20.39 Forearm.seek :garage @19:58:21.43 Hand.close @19:58:22.27 Forearm.seek :retract @19:58:23.30 Elbow.reconfigure TightElbow @19:58:23.35 Elbow.seek CC @19:58:26.82 Elbow.reconfigure LooseElbow @19:58:26.94 Forearm.reconfigure TightForearm @19:58:26.99 Forearm.seek CC @19:58:28.28 CC.seek :closed @19:58:32.48 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.53 @19:58:32.58 Hand.open @19:58:33.45 Forearm.reconfigure LooseForearm @19:58:33.50 Forearm.seek :retract @19:58:34.51 Flushing the Collection Valve::Manifold @19:58:34.66 CSV.dial CSR @19:58:35.22 Pulling 5ml of CSR.flush @19:58:35.23 CSR.select :flush @19:58:35.36 CS.seek 5ml @19:58:39.52 delay 3 seconds @19:58:42.55 ATV.dial :air3 @19:58:43.11 delay 3 seconds @19:58:46.19 AS.seek 0.604ml @19:58:49.22
delay 5 seconds @19:58:51.66 delay 3 seconds @19:58:54.24
CSR.select :air @19:58:54.42 CS.reconfigure CSfast @19:58:54.47 CS.seek 7.501ml @19:58:54.68 ATV.dial :bleach @19:58:55.32 AS.seek 0.589ml @19:58:58.62 delay 3 seconds @19:59:01.65 ATV.dial :ASV @19:59:01.84
CSV.dial :waste @19:59:02.68 ASV.dial :wasteR @19:59:03.30 AS.seek 0ml @19:59:03.46
CS.reconfigure CSconfig @19:59:03.51 CS.seek 0ml @19:59:23.88 Flushing lower manifold's waste1 port @19:59:24.02 CSV.dial :flush @19:59:24.55 CS.reconfigure CSfast @19:59:24.60 CS.seek 5ml @19:59:38.21 delay 5 seconds @19:59:43.24 CSV.dial :air @19:59:43.82 CS.seek 7ml @19:59:49.87 CSV.dial CTV @19:59:49.91
CTV.dial CSV @19:59:51.48 CBV.dial :waste1 @19:59:52.35 CS.seek 0ml @20:00:11.04 Flushing lower manifold's waste2b port @20:00:11.18 CSV.dial :flush @20:00:12.02 CS.seek 5ml @20:00:25.59 delay 5 seconds @20:00:30.62 CSV.dial :air @20:00:31.34 CS.seek 7ml @20:00:37.41 CSV.dial CTV @20:00:37.43
CTV.dial CSV @20:00:37.96 CBV.dial :waste2b @20:00:38.82 CS.seek 0ml @20:00:57.47 Flushing lower manifold's waste1 port @20:00:57.48 CSV.dial CSR @20:00:58.03 Pulling 4ml of CSR.mfbkill @20:00:58.04 CSR.select :mfbkill @20:00:58.16 CS.reconfigure CSconfig @20:00:58.21 CS.seek 4ml @20:01:02.19 delay 1:00 @20:01:09.54
delay 5 seconds @20:01:14.56 CSR.select :air @20:01:14.69 CS.reconfigure CSfast @20:01:14.74 CS.seek 10ml @20:01:31.54 CSV.dial CTV @20:01:31.57
CTV.dial CSV @20:01:32.10 CBV.dial :waste1 @20:01:32.97 CS.seek 0ml @20:01:59.51 Flushing lower manifold's waste2b port @20:01:59.52 CSV.dial CSR @20:02:00.10 Pulling 4ml of CSR.mfbkill @20:02:00.11 CSR.select :mfbkill @20:02:00.24 CS.reconfigure CSconfig @20:02:00.28 CS.seek 4ml @20:02:02.22 AFV.select :carrierSolution @20:02:02.33 delay 2 seconds @20:02:04.36 AS.reconfigure ASconfig @20:02:04.40 AS.seek 1ml @20:02:11.69
delay 5 seconds @20:02:16.71 CSR.select :air @20:02:16.85 CS.reconfigure CSfast @20:02:16.90 CS.seek 10ml @20:02:33.10 CSV.dial CTV @20:02:33.14
CTV.dial CSV @20:02:33.64 CBV.dial :waste2b @20:02:34.51 CS.seek 0ml @20:03:00.91 Killing upper manifold @20:03:00.92 CSV.dial CSR @20:03:01.49 Pulling 4ml of CSR.mfbkill @20:03:01.50 CSR.select :mfbkill @20:03:01.63 CS.reconfigure CSconfig @20:03:01.67 CS.seek 4ml @20:03:02.16 delay 1 second @20:03:03.18 AFV.select :holdingCoil @20:03:03.29 delay 1 second @20:03:04.31 ATV.dial ASV @20:03:04.33 ASV.dial :wasteR @20:03:04.35 AS.reconfigure ASslow1 @20:03:04.39 AS.seek 0ml @20:03:12.85
delay 5 seconds @20:03:17.87 CSR.select :air @20:03:18.00 CS.reconfigure CSfast @20:03:18.06 CS.seek 10ml @20:03:34.23 CSV.dial CBV @20:03:34.26
CBV.dial CSV @20:03:35.57 CTV.dial :air @20:03:37.08 CS.seek 0ml @20:04:03.37 delay 7 seconds @20:04:10.41 CS.seek 3ml @20:04:19.12 CSV.dial :waste @20:04:19.63 CS.seek 0ml @20:04:28.19 Flushing upper manifold @20:04:28.33 CSV.dial :flush @20:04:28.82 CS.seek 5ml @20:04:42.48 delay 5 seconds @20:04:47.50 CSV.dial :air @20:04:48.08 CS.seek 7ml @20:04:54.10 CSV.dial CBV @20:04:54.13
CBV.dial CSV @20:04:55.43 CTV.dial :air @20:04:55.45 CS.seek 0ml @20:05:14.20 delay 7 seconds @20:05:21.23 CS.seek 3ml @20:05:30.06 CSV.dial :waste @20:05:30.55 CS.seek 0ml @20:05:39.19 CSV.dial :flush @20:05:39.70 CS.seek 3ml @20:05:48.37 delay 5 seconds @20:05:53.40 CSV.dial :air @20:05:54.11 CS.seek 5ml @20:06:00.15 CSV.dial :air @20:06:00.17 CS.seek 0ml @20:06:14.01 delay 7 seconds @20:06:21.04 CS.seek 3ml @20:06:25.79 delay 20 seconds @20:06:29.69
CSV.dial :waste @20:06:30.52 CS.seek 0ml @20:06:38.98 Flushing lower manifold's waste1 port @20:06:39.12 CSV.dial :flush @20:06:39.63 CS.seek 5ml @20:06:45.82 ATV.dial :air3 @20:06:47.33 AS.seek 0.59ml @20:06:53.44
delay 5 seconds @20:06:58.47 CSV.dial :air @20:06:59.05 CS.seek 7ml @20:07:05.13 CSV.dial CTV @20:07:05.16
CTV.dial CSV @20:07:06.75 CBV.dial :waste1 @20:07:07.27 CS.seek 0ml @20:07:25.95 Flushing lower manifold's waste2b port @20:07:26.09 CSV.dial :flush @20:07:26.92 CS.seek 5ml @20:07:40.62 delay 5 seconds @20:07:45.64 CSV.dial :air @20:07:46.26 CS.seek 7.001ml @20:07:52.35 CSV.dial CTV @20:07:52.38
CTV.dial CSV @20:07:52.87 CBV.dial :waste2b @20:07:53.74 CS.seek 0ml @20:08:12.67 Garaging FlushPuck in Collection Clamp @20:08:12.75 Elbow.reconfigure TightElbow @20:08:12.80 Elbow.seek CC @20:08:13.06 Elbow.reconfigure LooseElbow @20:08:13.17 Forearm.seek CC @20:08:14.18 Hand.close @20:08:14.89 CC.seek :open @20:08:19.26 Forearm.seek :retract @20:08:20.25 Elbow.reconfigure TightElbow @20:08:20.29 Elbow.seek :garage @20:08:23.48 Elbow.reconfigure LooseElbow @20:08:23.59 Forearm.reconfigure TightForearm @20:08:23.64 Forearm.seek :garage @20:08:24.80 Hand.open @20:08:25.59 Forearm.reconfigure LooseForearm @20:08:25.64 Forearm.seek :retract @20:08:26.67 Starting finalPurge @20:08:26.68 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:08:26.75 Hand.open @20:08:27.07 Elbow.reconfigure TightElbow @20:08:27.12 Elbow.seek FlushPuck::Garage @20:08:27.28 Elbow.reconfigure LooseElbow @20:08:27.39 Forearm.seek :garage @20:08:28.45 Hand.close @20:08:29.27 Forearm.seek :retract @20:08:30.29 Elbow.reconfigure TightElbow @20:08:30.33 Elbow.seek CC @20:08:33.31 Elbow.reconfigure LooseElbow @20:08:33.42 Forearm.reconfigure TightForearm @20:08:33.46 Forearm.seek CC @20:08:34.76 CC.seek :closed @20:08:38.99 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.53 @20:08:39.08 Hand.open @20:08:39.95 Forearm.reconfigure LooseForearm @20:08:40.00 Forearm.seek :retract @20:08:41.55 Flushing the Collection Valve::Manifold @20:08:41.69 CSV.dial CSR @20:08:42.26 Pulling 5ml of CSR.flush @20:08:42.27 CSR.select :flush @20:08:42.39 CS.reconfigure CSconfig @20:08:42.44 CS.seek 5ml @20:08:46.91 delay 3 seconds @20:08:49.94 ATV.dial :ASV @20:08:51.44 ASV.dial :wasteR @20:08:51.53 AS.seek 0.09ml @20:08:56.42
delay 5 seconds @20:09:01.44 CSR.select :air @20:09:01.62 CS.reconfigure CSfast @20:09:01.67 CS.seek 7.5ml @20:09:09.03 CSV.dial :waste @20:09:10.51 CS.reconfigure CSconfig @20:09:10.56 CS.seek 0ml @20:09:30.67 Flushing lower manifold's waste1 port @20:09:30.80 CSV.dial :flush @20:09:31.31 CS.reconfigure CSfast @20:09:31.36 CS.seek 5ml @20:09:45.14 delay 5 seconds @20:09:50.16 CSV.dial :air @20:09:50.75 CS.seek 7.001ml @20:09:56.89 CSV.dial CTV @20:09:56.92
CTV.dial CSV @20:09:57.42 CBV.dial :waste1 @20:09:58.28 CS.seek 0ml @20:10:17.01 Flushing lower manifold's waste2b port @20:10:17.15 CSV.dial :flush @20:10:17.99 CS.seek 5ml @20:10:31.56 delay 5 seconds @20:10:32.35 delay 30 seconds @20:10:36.59
CSV.dial :air @20:10:37.19 CS.seek 7ml @20:10:43.26 CSV.dial CTV @20:10:43.29
CTV.dial CSV @20:10:43.78 CBV.dial :waste2b @20:10:44.64 CS.seek 0ml @20:11:02.38 ATV.dial :extSrc @20:11:03.16 delay 3 seconds @20:11:03.35
Disinfecting external Intake with kill @20:11:03.48 CSV.dial CSR @20:11:04.04 Pulling 8ml of CSR.kill @20:11:04.05 CSR.select :kill @20:11:04.18 CS.seek 8ml @20:11:06.18 ATV.dial :ElutionWater @20:11:07.30 AS.seek 0.12ml @20:11:13.72 ATV.dial :air3 @20:11:14.34 AS.seek 0.13ml @20:11:16.66 delay 2 seconds @20:11:18.68 ATV.dial :bleach @20:11:19.15 AS.seek 0.23ml @20:11:25.53
delay 5 seconds @20:11:30.56 CSR.select :air @20:11:30.68 CS.seek 10ml @20:11:36.82 CSV.dial CBV @20:11:36.85
CBV.dial CSV @20:11:38.03 CTV.dial :intake @20:11:38.88 Intake.select :open @20:11:39.00 CS.seek 2ml @20:11:39.61 delay 3 seconds @20:11:42.63 ATV.dial :air3 @20:11:43.22 delay 3 seconds @20:11:46.30 AS.seek 0.255ml @20:11:51.75 delay 3 seconds @20:11:54.78 ATV.dial :bleach @20:11:55.42 AS.seek 0.24ml @20:11:58.79 delay 3 seconds @20:12:01.00
Intake.select :close @20:12:01.11 CTV.dial :air @20:12:01.81 ATV.dial :ASV @20:12:01.98
CS.reconfigure CSconfig @20:12:02.03 CS.seek 0ml @20:12:02.83 ASV.dial :SPEdelivery @20:12:03.21 AFV.select :carrierSolution @20:12:03.33 delay 2 seconds @20:12:05.35 AS.reconfigure ASconfig @20:12:05.40 AS.seek 0.5ml @20:12:08.16
CSV.dial CSR @20:12:09.40 Pulling 5ml of CSR.flush @20:12:09.92 CSR.select :flush @20:12:10.06 CS.seek 5ml @20:12:20.61 delay 1 second @20:12:21.63 AFV.select :holdingCoil @20:12:21.79 AS.reconfigure ASslow1 @20:12:21.85 AS.seek 0.17ml @20:12:23.89
delay 5 seconds @20:12:28.91 CSR.select :air @20:12:29.03 CS.seek 10ml @20:12:42.81 CSV.dial CBV @20:12:43.98 CS.seek 0ml @20:13:10.53 Garaging FlushPuck in Collection Clamp @20:13:10.62 Elbow.reconfigure TightElbow @20:13:10.67 Elbow.seek CC @20:13:10.91 Elbow.reconfigure LooseElbow @20:13:11.02 Forearm.seek CC @20:13:12.03 Hand.close @20:13:12.77 CC.seek :open @20:13:17.14 Forearm.seek :retract @20:13:18.18 Elbow.reconfigure TightElbow @20:13:18.23 Elbow.seek :garage @20:13:21.38 Elbow.reconfigure LooseElbow @20:13:21.48 Forearm.reconfigure TightForearm @20:13:21.53 Forearm.seek :garage @20:13:22.74 Hand.open @20:13:23.59 Forearm.reconfigure LooseForearm @20:13:23.64 Forearm.seek :retract @20:13:24.70 Completed [mbaPhase] begun 18:34:11 @20:13:24.72 Extending 18tun1 until completion of MFBPCR @20:13:28.53 SPE.hold 2:46:40,95C @20:13:28.56 delay 3:00 @20:16:28.60 AFV.select :carrierSolution @20:16:28.71 delay 2 seconds @20:16:30.74 AS.reconfigure ASconfig @20:16:30.79 AS.seek 1ml @20:17:18.79 delay 1 second @20:17:19.81 AFV.select :holdingCoil @20:17:19.92 delay 1 second @20:17:20.94 ASV.dial :wasteD @20:17:22.01 AS.seek 0ml @20:18:19.82 Priming Eluate @20:18:19.84 AFV.select :carrierSolution @20:18:19.95 delay 2 seconds @20:18:21.98 AS.seek 0.5ml @20:18:51.02 delay 1 second @20:18:52.04 AFV.select :holdingCoil @20:18:52.14 ATV.dial ASV @20:18:52.16 ASV.dial :SPErecovery @20:18:52.75 Swish #1 of 2 @20:18:52.84 AS.reconfigure ASslow0 @20:18:52.89 AS.seek 0.6ml @20:19:03.37 AS.seek 0.5ml @20:19:13.79 Swish #2 of 2 @20:19:13.88 AS.seek 0.6ml @20:19:24.35 AS.seek 0.5ml @20:19:34.75 delay 3 seconds @20:19:37.83 AS.reconfigure ASslow1 @20:19:37.88 AS.seek 0.684ml @20:20:15.30 delay 3 seconds @20:20:18.33 Eluate primed @20:20:18.34 ATV.dial :waste2 @20:20:18.97 AS.reconfigure ASnorm @20:20:19.02 AS.seek 0ml @20:20:53.80 SPE.coast @20:20:53.88 ASV.dial :SPErecovery @20:20:53.91 Setting up PCR for Rp -- writing /var/log/esp/Rp18sep1620h200ml.pcr @20:20:53.92 Building PCR Slug from ASV with MM2 mix, PP3 primer @20:20:53.94 Priming with carrier @20:20:53.95 ATV.dial ARV @20:20:54.90 ARV.dial :carrier @20:20:55.27 AS.seek 0.5ml @20:21:20.83 AFV.select :carrierSolution @20:21:20.95 delay 2 seconds @20:21:22.98 AS.reconfigure ASconfig @20:21:23.03 AS.seek 1ml @20:21:52.10 delay 1 second @20:21:53.13 AFV.select :holdingCoil @20:21:53.24 delay 1 second @20:21:54.26 ATV.dial :waste @20:21:55.75 AS.reconfigure ASnorm @20:21:55.80 AS.seek :empty @20:22:46.52 AFV.select :carrierSolution @20:22:46.64 delay 2 seconds @20:22:48.67 AS.reconfigure ASconfig @20:22:48.72 AS.seek 0.5ml @20:23:17.82 delay 1 second @20:23:18.84 AFV.select :holdingCoil @20:23:18.95 Building Slug @20:23:18.96 ATV.dial :ARV @20:23:20.55 delay 2 seconds @20:23:22.57 ARV.dial :air1 @20:23:22.99 delay 2 seconds @20:23:25.53 AS.reconfigure ASslow2 @20:23:25.66 AS.seek 0.506ml @20:23:31.87 delay 2 seconds @20:23:33.89 ARV.dial :MM2 @20:23:35.42 delay 2 seconds @20:23:37.45 Adding MM2 @20:23:37.53 AS.seek 0.524ml @20:23:55.81 delay 25 seconds @20:24:20.83 ARV.dial :PP3 @20:24:21.23 Adding PP3 @20:24:21.24 delay 2 seconds @20:24:23.33 AS.seek 0.53ml @20:24:29.70 delay 10 seconds @20:24:39.72 ARV.dial :air2 @20:24:40.12 adding ARV air @20:24:40.13 delay 2 seconds @20:24:42.22 AS.seek 0.536ml @20:24:48.62 delay 5 seconds @20:24:53.64 ARV.dial :carrier @20:24:54.95 delay 7 seconds @20:25:02.04 AS.seek 0.594ml @20:26:00.62 delay 5 seconds @20:26:05.64 ATV.dial :ASV @20:26:06.05 delay 2 seconds @20:26:08.14 AS.seek 0.6ml @20:26:14.36 delay 4 seconds @20:26:18.39 ATV.dial ARV @20:26:18.94 delay 2 seconds @20:26:20.96 ARV.dial :carrier @20:26:20.98 delay 2 seconds @20:26:23.07 AS.seek 0.65ml @20:27:13.68 delay 4 seconds @20:27:17.71 ATV.dial PCR @20:27:18.32 ASV.dial :waste1 @20:27:19.11 ARV.dial :air1 @20:27:19.53 Positioning Slug by 0.324ml @20:27:19.62 AS.seek 0.326ml @20:32:45.85 delay 5 seconds @20:32:50.88 ATV.dial ARV @20:32:51.64 ASV.dial :PCRlock @20:32:52.01 delay 5 seconds @20:32:57.04 Running Rp Profile60C300 thermocycling @20:32:57.05 PCR.program Profile60C300 @20:32:57.19 Profile60C300 should complete in < 1:45:21 @20:32:57.20 PCR.run 2:16:41.25 @21:57:50.40 PCR.off @21:57:51.97 PCR for Rp completed -- closed /var/log/esp/Rp18sep1620h200ml.pcr @21:57:52.04 Cleaning PCR @21:57:52.05 AFV.select :carrierSolution @21:57:52.16 delay 2 seconds @21:57:54.19 AS.reconfigure ASconfig @21:57:54.24 AS.seek 0.5ml @21:58:04.53 delay 1 second @21:58:05.55 AFV.select :holdingCoil @21:58:05.65 ASV.dial :waste1 @21:58:06.03 ATV.dial :air3 @21:58:07.30 AS.reconfigure ASslow1 @21:58:07.36 AS.seek 0.51ml @21:58:09.64 delay 2 seconds @21:58:11.66 ATV.dial :bleach @21:58:12.29 AS.seek 0.81ml @21:59:12.89 delay 3 seconds @21:59:15.92 ATV.dial :air3 @21:59:16.48 delay 3 seconds @21:59:19.56 AS.seek 0.835ml @21:59:24.86 delay 3 seconds @21:59:27.89 ATV.dial :bleach @21:59:28.51 AS.seek 0.82ml @21:59:31.84 delay 3 seconds @21:59:34.87 ARV.dial :waste @21:59:35.51 delay 3 seconds @21:59:38.53 ATV.dial :ARV @21:59:39.40 AS.reconfigure ASconfig @21:59:39.46 AS.seek 0.47ml @21:59:59.91 AFV.select :carrierSolution @22:00:00.03 delay 2 seconds @22:00:02.06 AS.seek 1ml @22:00:32.84 delay 1 second @22:00:33.86 AFV.select :holdingCoil @22:00:33.97 delay 1 second @22:00:35.00 AS.reconfigure ASfast @22:00:35.04 AS.seek 0ml @22:01:02.41 AFV.select :carrierSolution @22:01:03.08 delay 2 seconds @22:01:05.11 AS.reconfigure ASconfig @22:01:05.16 AS.seek 1ml @22:02:02.94 delay 1 second @22:02:03.96 AFV.select :holdingCoil @22:02:04.07 delay 1 second @22:02:05.10 AS.reconfigure ASfast @22:02:05.15 AS.seek 0ml @22:02:32.50 AFV.select :carrierSolution @22:02:32.61 delay 2 seconds @22:02:34.65 AS.reconfigure ASconfig @22:02:34.70 AS.seek 1ml @22:03:32.52 delay 1 second @22:03:33.55 AFV.select :holdingCoil @22:03:33.67 delay 1 second @22:03:34.70 AS.reconfigure ASfast @22:03:34.75 AS.seek 0ml @22:04:02.09 ARV.dial between carrier and air1 @22:04:02.62 ATV.dial :air3 @22:04:03.91 AS.reconfigure ASslow1 @22:04:03.96 AS.seek 0.01ml @22:04:06.41 delay 2 seconds @22:04:08.44 ATV.dial :bleach @22:04:09.08 AS.seek 0.81ml @22:06:50.33 delay 3 seconds @22:06:53.36 ATV.dial :air3 @22:06:53.92 delay 3 seconds @22:06:57.00 AS.seek 0.835ml @22:07:02.46 delay 3 seconds @22:07:05.48 ATV.dial :bleach @22:07:06.11 AS.seek 0.82ml @22:07:09.37 delay 3 seconds @22:07:12.40 ATV.dial PCR @22:07:12.79 AS.reconfigure ASconfig @22:07:12.84 AS.seek :empty @22:08:00.31 PCR.program :bleachProfile @22:08:00.37 PCR program should complete in < 2:45 @22:08:00.38 PCR.run 10:00 @22:10:59.09 PCR.off @22:10:59.11 AFV.select :carrierSolution @22:10:59.23 delay 2 seconds @22:11:01.26 AS.seek 1ml @22:11:59.09 delay 1 second @22:12:00.11 AFV.select :holdingCoil @22:12:00.22 delay 1 second @22:12:01.25 AS.reconfigure ASfast @22:12:01.30 AS.seek 0ml @22:12:28.62 delay 3 seconds @22:12:31.65 AFV.select :carrierSolution @22:12:31.76 delay 2 seconds @22:12:33.79 AS.reconfigure ASconfig @22:12:33.84 AS.seek 1ml @22:13:31.58 delay 1 second @22:13:32.61 AFV.select :holdingCoil @22:13:32.72 delay 1 second @22:13:33.75 AS.reconfigure ASfast @22:13:33.80 AS.seek 0ml @22:14:01.12 delay 3 seconds @22:14:04.15 AFV.select :carrierSolution @22:14:04.26 delay 2 seconds @22:14:06.29 AS.reconfigure ASconfig @22:14:06.34 AS.seek 1ml @22:15:04.22 delay 1 second @22:15:05.24 AFV.select :holdingCoil @22:15:05.35 delay 1 second @22:15:06.38 AS.reconfigure ASfast @22:15:06.44 AS.seek 0ml @22:15:33.78 delay 3 seconds @22:15:36.81 AFV.select :carrierSolution @22:15:36.92 delay 2 seconds @22:15:38.95 AS.reconfigure ASconfig @22:15:39.00 AS.seek 1ml @22:16:36.75 delay 1 second @22:16:37.77 AFV.select :holdingCoil @22:16:37.88 delay 1 second @22:16:38.90 AS.reconfigure ASfast @22:16:38.95 AS.seek 0ml @22:17:06.30 delay 3 seconds @22:17:09.33 AFV.select :carrierSolution @22:17:09.44 delay 2 seconds @22:17:11.47 AS.reconfigure ASconfig @22:17:11.53 AS.seek 1ml @22:18:09.34 delay 1 second @22:18:10.36 AFV.select :holdingCoil @22:18:10.47 delay 1 second @22:18:11.50 AS.reconfigure ASnorm @22:18:11.55 AS.seek 0ml @22:19:02.27 Setting up PCR for Ss -- writing /var/log/esp/Ss18sep1622h200ml.pcr @22:19:02.29 Building PCR Slug from ASV with MM1 mix, PP2 primer @22:19:02.31 Priming with carrier @22:19:02.32 ATV.dial ARV @22:19:02.92 ARV.dial :carrier @22:19:03.28 AS.seek 0.5ml @22:19:28.84 AFV.select :carrierSolution @22:19:28.96 delay 2 seconds @22:19:30.99 AS.reconfigure ASconfig @22:19:31.04 AS.seek 1ml @22:20:00.09 delay 1 second @22:20:01.11 AFV.select :holdingCoil @22:20:01.23 delay 1 second @22:20:02.25 ATV.dial :waste @22:20:04.45 AS.reconfigure ASnorm @22:20:04.50 AS.seek :empty @22:20:55.22 AFV.select :carrierSolution @22:20:55.34 delay 2 seconds @22:20:57.37 AS.reconfigure ASconfig @22:20:57.42 AS.seek 0.5ml @22:21:26.53 delay 1 second @22:21:27.55 AFV.select :holdingCoil @22:21:27.66 Building Slug @22:21:27.67 ATV.dial :ARV @22:21:29.26 delay 2 seconds @22:21:31.28 ARV.dial :air1 @22:21:31.69 delay 2 seconds @22:21:33.78 AS.reconfigure ASslow2 @22:21:33.83 AS.seek 0.506ml @22:21:40.22 delay 2 seconds @22:21:42.25 ARV.dial :MM1 @22:21:42.85 delay 2 seconds @22:21:44.88 Adding MM1 @22:21:44.95 AS.seek 0.524ml @22:22:03.26 delay 25 seconds @22:22:28.29 ARV.dial :PP2 @22:22:28.70 Adding PP2 @22:22:28.71 delay 2 seconds @22:22:30.80 AS.seek 0.53ml @22:22:37.03 delay 10 seconds @22:22:47.06 ARV.dial :air2 @22:22:47.46 adding ARV air @22:22:47.47 delay 2 seconds @22:22:49.55 AS.seek 0.536ml @22:22:55.73 delay 5 seconds @22:23:00.76 ARV.dial :carrier @22:23:02.06 delay 7 seconds @22:23:09.15 AS.seek 0.594ml @22:24:07.73 delay 5 seconds @22:24:12.76 ATV.dial :ASV @22:24:13.15 delay 2 seconds @22:24:15.24 AS.seek 0.6ml @22:24:21.72 delay 4 seconds @22:24:25.75 ATV.dial ARV @22:24:26.29 delay 2 seconds @22:24:28.31 ARV.dial :carrier @22:24:28.33 delay 2 seconds @22:24:30.41 AS.seek 0.65ml @22:25:21.34 delay 4 seconds @22:25:25.37 ATV.dial PCR @22:25:25.97 ASV.dial :waste1 @22:25:25.99 ARV.dial :air1 @22:25:26.41 Positioning Slug by 0.324ml @22:25:26.50 AS.seek 0.326ml @22:30:52.70 delay 5 seconds @22:30:57.72 ATV.dial ARV @22:30:58.48 ASV.dial :PCRlock @22:30:58.88 delay 5 seconds @22:31:03.91 Running Ss Profile60C350 thermocycling @22:31:03.92 PCR.program Profile60C350 @22:31:04.06 Profile60C350 should complete in < 1:48:05 @22:31:04.07 PCR.run 2:20:06.25 @23:56:24.58 PCR.off @23:56:26.73 PCR for Ss completed -- closed /var/log/esp/Ss18sep1622h200ml.pcr @23:56:26.80 Cleaning PCR @23:56:26.81 AFV.select :carrierSolution @23:56:26.91 delay 2 seconds @23:56:28.95 AS.reconfigure ASconfig @23:56:29.00 AS.seek 0.5ml @23:56:39.28 delay 1 second @23:56:40.30 AFV.select :holdingCoil @23:56:40.40 ASV.dial :waste1 @23:56:40.78 ATV.dial :air3 @23:56:42.07 AS.reconfigure ASslow1 @23:56:42.12 AS.seek 0.51ml @23:56:44.41 delay 2 seconds @23:56:46.43 ATV.dial :bleach @23:56:47.06 AS.seek 0.81ml @23:57:47.64 delay 3 seconds @23:57:50.66 ATV.dial :air3 @23:57:51.21 delay 3 seconds @23:57:54.85 AS.seek 0.835ml @23:58:00.12 delay 3 seconds @23:58:03.15 ATV.dial :bleach @23:58:03.75 AS.seek 0.82ml @23:58:07.06 delay 3 seconds @23:58:10.09 ARV.dial :waste @23:58:10.70 delay 3 seconds @23:58:13.72 ATV.dial :ARV @23:58:14.60 AS.reconfigure ASconfig @23:58:14.66 AS.seek 0.47ml @23:58:35.06 AFV.select :carrierSolution @23:58:35.18 delay 2 seconds @23:58:37.21 AS.seek 1ml @23:59:08.00 delay 1 second @23:59:09.02 AFV.select :holdingCoil @23:59:09.13 delay 1 second @23:59:10.16 AS.reconfigure ASfast @23:59:10.21 AS.seek 0ml @23:59:37.56 AFV.select :carrierSolution @23:59:37.68 delay 2 seconds @23:59:39.71 AS.reconfigure ASconfig @23:59:39.76 AS.seek 1ml @00:00:37.59CEST17-Sep-18 delay 1 second @00:00:38.61 AFV.select :holdingCoil @00:00:38.72 delay 1 second @00:00:39.75 AS.reconfigure ASfast @00:00:39.80 AS.seek 0ml @00:01:07.17 AFV.select :carrierSolution @00:01:07.29 delay 2 seconds @00:01:09.32 AS.reconfigure ASconfig @00:01:09.37 AS.seek 1ml @00:02:07.14 delay 1 second @00:02:08.16 AFV.select :holdingCoil @00:02:08.27 delay 1 second @00:02:09.30 AS.reconfigure ASfast @00:02:09.34 AS.seek 0ml @00:02:36.70 ARV.dial between carrier and air1 @00:02:37.21 ATV.dial :air3 @00:02:38.47 AS.reconfigure ASslow1 @00:02:38.53 AS.seek 0.01ml @00:02:40.89 delay 2 seconds @00:02:42.92 ATV.dial :bleach @00:02:43.56 AS.seek 0.81ml @00:05:24.78 delay 3 seconds @00:05:27.81 ATV.dial :air3 @00:05:28.36 delay 3 seconds @00:05:31.44 AS.seek 0.835ml @00:05:36.72 delay 3 seconds @00:05:39.75 ATV.dial :bleach @00:05:40.38 AS.seek 0.82ml @00:05:43.68 delay 3 seconds @00:05:46.71 ATV.dial PCR @00:05:47.10 AS.reconfigure ASconfig @00:05:47.15 AS.seek :empty @00:06:34.60 PCR.program :bleachProfile @00:06:34.65 PCR program should complete in < 2:45 @00:06:34.67 PCR.run 10:00 @00:09:33.42 PCR.off @00:09:33.45 AFV.select :carrierSolution @00:09:33.57 delay 2 seconds @00:09:35.60 AS.seek 1ml @00:10:33.39 delay 1 second @00:10:34.41 AFV.select :holdingCoil @00:10:34.52 delay 1 second @00:10:35.55 AS.reconfigure ASfast @00:10:35.60 AS.seek 0ml @00:11:02.93 delay 3 seconds @00:11:05.96 AFV.select :carrierSolution @00:11:06.07 delay 2 seconds @00:11:08.10 AS.reconfigure ASconfig @00:11:08.15 AS.seek 1ml @00:12:05.90 delay 1 second @00:12:06.92 AFV.select :holdingCoil @00:12:07.03 delay 1 second @00:12:08.06 AS.reconfigure ASfast @00:12:08.10 AS.seek 0ml @00:12:35.46 delay 3 seconds @00:12:38.49 AFV.select :carrierSolution @00:12:38.60 delay 2 seconds @00:12:40.63 AS.reconfigure ASconfig @00:12:40.68 AS.seek 1ml @00:13:38.43 delay 1 second @00:13:39.45 AFV.select :holdingCoil @00:13:39.56 delay 1 second @00:13:40.59 AS.reconfigure ASfast @00:13:40.64 AS.seek 0ml @00:14:07.96 delay 3 seconds @00:14:10.99 AFV.select :carrierSolution @00:14:11.10 delay 2 seconds @00:14:13.13 AS.reconfigure ASconfig @00:14:13.19 AS.seek 1ml @00:15:10.98 delay 1 second @00:15:12.00 AFV.select :holdingCoil @00:15:12.11 delay 1 second @00:15:13.14 AS.reconfigure ASfast @00:15:13.19 AS.seek 0ml @00:15:40.54 delay 3 seconds @00:15:43.56 AFV.select :carrierSolution @00:15:43.67 delay 2 seconds @00:15:45.70 AS.reconfigure ASconfig @00:15:45.75 AS.seek 1ml @00:16:44.09 delay 1 second @00:16:45.11 AFV.select :holdingCoil @00:16:45.22 delay 1 second @00:16:46.25 AS.reconfigure ASnorm @00:16:46.30 AS.seek 0ml @00:17:37.03 Setting up PCR for Bb -- writing /var/log/esp/Bb18sep1700h200ml.pcr @00:17:37.05 Building PCR Slug from ASV with MM3 mix, PP5 primer @00:17:37.06 Priming with carrier @00:17:37.07 ATV.dial ARV @00:17:37.67 ARV.dial :carrier @00:17:38.04 AS.seek 0.5ml @00:18:03.68 AFV.select :carrierSolution @00:18:03.80 delay 2 seconds @00:18:05.83 AS.reconfigure ASconfig @00:18:05.88 AS.seek 1ml @00:18:34.89 delay 1 second @00:18:35.91 AFV.select :holdingCoil @00:18:36.02 delay 1 second @00:18:37.04 ATV.dial :waste @00:18:38.68 AS.reconfigure ASnorm @00:18:38.73 AS.seek :empty @00:19:29.51 AFV.select :carrierSolution @00:19:29.63 delay 2 seconds @00:19:31.66 AS.reconfigure ASconfig @00:19:31.70 AS.seek 0.5ml @00:20:00.79 delay 1 second @00:20:01.81 AFV.select :holdingCoil @00:20:01.92 Building Slug @00:20:01.93 ATV.dial :ARV @00:20:03.54 delay 2 seconds @00:20:05.56 ARV.dial :air1 @00:20:06.02 delay 2 seconds @00:20:08.10 AS.reconfigure ASslow2 @00:20:08.16 AS.seek 0.506ml @00:20:14.53 delay 2 seconds @00:20:16.56 ARV.dial :MM3 @00:20:17.65 delay 2 seconds @00:20:19.68 Adding MM3 @00:20:19.76 AS.seek 0.524ml @00:20:38.31 delay 25 seconds @00:21:03.34 ARV.dial :PP5 @00:21:03.74 Adding PP5 @00:21:03.75 delay 2 seconds @00:21:05.84 AS.seek 0.53ml @00:21:12.03 delay 10 seconds @00:21:22.06 ARV.dial :air3 @00:21:22.46 adding ARV air @00:21:22.47 delay 2 seconds @00:21:24.55 AS.seek 0.536ml @00:21:30.81 delay 5 seconds @00:21:35.84 ARV.dial :carrier @00:21:37.12 delay 7 seconds @00:21:44.21 AS.seek 0.594ml @00:22:42.85 delay 5 seconds @00:22:47.88 ATV.dial :ASV @00:22:48.29 delay 2 seconds @00:22:50.37 AS.seek 0.6ml @00:22:56.81 delay 4 seconds @00:23:00.84 ATV.dial ARV @00:23:01.38 delay 2 seconds @00:23:03.40 ARV.dial :carrier @00:23:03.42 delay 2 seconds @00:23:05.50 AS.seek 0.65ml @00:23:56.24 delay 4 seconds @00:24:00.27 ATV.dial PCR @00:24:00.87 ASV.dial :waste1 @00:24:00.89 ARV.dial :air1 @00:24:01.34 Positioning Slug by 0.324ml @00:24:01.42 AS.seek 0.326ml @00:29:27.68 delay 5 seconds @00:29:32.71 ATV.dial ARV @00:29:33.48 ASV.dial :PCRlock @00:29:33.88 delay 5 seconds @00:29:38.91 Running Bb Profile60C300 thermocycling @00:29:38.92 PCR.program Profile60C300 @00:29:39.06 Profile60C300 should complete in < 1:45:21 @00:29:39.07 PCR.run 2:16:41.25 @01:54:16.20 PCR.off @01:54:17.81 PCR for Bb completed -- closed /var/log/esp/Bb18sep1700h200ml.pcr @01:54:17.88 Cleaning PCR @01:54:17.89 AFV.select :carrierSolution @01:54:18.01 delay 2 seconds @01:54:20.04 AS.reconfigure ASconfig @01:54:20.09 AS.seek 0.5ml @01:54:30.37 delay 1 second @01:54:31.39 AFV.select :holdingCoil @01:54:31.49 ASV.dial :waste1 @01:54:31.87 ATV.dial :air3 @01:54:33.15 AS.reconfigure ASslow1 @01:54:33.21 AS.seek 0.51ml @01:54:35.49 delay 2 seconds @01:54:37.51 ATV.dial :bleach @01:54:38.15 AS.seek 0.81ml @01:55:38.74 delay 3 seconds @01:55:41.77 ATV.dial :air3 @01:55:42.33 delay 3 seconds @01:55:45.41 AS.seek 0.835ml @01:55:50.63 delay 3 seconds @01:55:53.66 ATV.dial :bleach @01:55:54.85 AS.seek 0.82ml @01:55:58.13 delay 3 seconds @01:56:01.16 ARV.dial :waste @01:56:01.78 delay 3 seconds @01:56:04.80 ATV.dial :ARV @01:56:05.78 AS.reconfigure ASconfig @01:56:05.83 AS.seek 0.47ml @01:56:26.26 AFV.select :carrierSolution @01:56:26.38 delay 2 seconds @01:56:28.41 AS.seek 1ml @01:56:59.15 delay 1 second @01:57:00.17 AFV.select :holdingCoil @01:57:00.28 delay 1 second @01:57:01.31 AS.reconfigure ASfast @01:57:01.36 AS.seek 0ml @01:57:28.68 AFV.select :carrierSolution @01:57:28.80 delay 2 seconds @01:57:30.83 AS.reconfigure ASconfig @01:57:30.88 AS.seek 1ml @01:58:28.66 delay 1 second @01:58:29.68 AFV.select :holdingCoil @01:58:29.79 delay 1 second @01:58:30.82 AS.reconfigure ASfast @01:58:30.87 AS.seek 0ml @01:58:58.21 AFV.select :carrierSolution @01:58:58.33 delay 2 seconds @01:59:00.36 AS.reconfigure ASconfig @01:59:00.41 AS.seek 1ml @01:59:58.24 delay 1 second @01:59:59.26 AFV.select :holdingCoil @01:59:59.37 delay 1 second @02:00:00.40 AS.reconfigure ASfast @02:00:00.45 AS.seek 0ml @02:00:27.80 ARV.dial between carrier and air1 @02:00:28.30 ATV.dial :air3 @02:00:29.55 AS.reconfigure ASslow1 @02:00:29.61 AS.seek 0.01ml @02:00:31.98 delay 2 seconds @02:00:34.00 ATV.dial :bleach @02:00:34.63 AS.seek 0.81ml @02:03:15.79 delay 3 seconds @02:03:18.82 ATV.dial :air3 @02:03:19.37 delay 3 seconds @02:03:22.46 AS.seek 0.835ml @02:03:27.87 delay 3 seconds @02:03:30.90 ATV.dial :bleach @02:03:31.54 AS.seek 0.82ml @02:03:34.90 delay 3 seconds @02:03:37.93 ATV.dial PCR @02:03:38.34 AS.reconfigure ASconfig @02:03:38.43 AS.seek :empty @02:04:25.91 PCR.program :bleachProfile @02:04:25.96 PCR program should complete in < 2:45 @02:04:25.97 PCR.run 10:00 @02:07:24.73 PCR.off @02:07:24.76 AFV.select :carrierSolution @02:07:24.87 delay 2 seconds @02:07:26.90 AS.seek 1ml @02:08:24.77 delay 1 second @02:08:25.79 AFV.select :holdingCoil @02:08:25.90 delay 1 second @02:08:26.93 AS.reconfigure ASfast @02:08:26.98 AS.seek 0ml @02:08:54.32 delay 3 seconds @02:08:57.34 AFV.select :carrierSolution @02:08:57.45 delay 2 seconds @02:08:59.48 AS.reconfigure ASconfig @02:08:59.53 AS.seek 1ml @02:09:57.27 delay 1 second @02:09:58.29 AFV.select :holdingCoil @02:09:58.40 delay 1 second @02:09:59.43 AS.reconfigure ASfast @02:09:59.48 AS.seek 0ml @02:10:26.82 delay 3 seconds @02:10:29.85 AFV.select :carrierSolution @02:10:29.96 delay 2 seconds @02:10:31.99 AS.reconfigure ASconfig @02:10:32.05 AS.seek 1ml @02:11:29.79 delay 1 second @02:11:30.81 AFV.select :holdingCoil @02:11:30.92 delay 1 second @02:11:31.95 AS.reconfigure ASfast @02:11:32.00 AS.seek 0ml @02:11:59.35 delay 3 seconds @02:12:02.38 AFV.select :carrierSolution @02:12:02.49 delay 2 seconds @02:12:04.52 AS.reconfigure ASconfig @02:12:04.57 AS.seek 1ml @02:13:02.30 delay 1 second @02:13:03.32 AFV.select :holdingCoil @02:13:03.43 delay 1 second @02:13:04.46 AS.reconfigure ASfast @02:13:04.51 AS.seek 0ml @02:13:31.85 delay 3 seconds @02:13:34.88 AFV.select :carrierSolution @02:13:34.99 delay 2 seconds @02:13:37.02 AS.reconfigure ASconfig @02:13:37.07 AS.seek 1ml @02:14:34.85 delay 1 second @02:14:35.87 AFV.select :holdingCoil @02:14:35.98 delay 1 second @02:14:37.01 AS.reconfigure ASnorm @02:14:37.63 AS.seek 0ml @02:15:28.34 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118sep1702h200ml.pcr @02:15:28.36 Building PCR Slug from ASV with MM2 mix, PP4 primer @02:15:28.37 Priming with carrier @02:15:28.38 ATV.dial ARV @02:15:28.97 ARV.dial :carrier @02:15:29.34 AS.seek 0.5ml @02:15:54.93 AFV.select :carrierSolution @02:15:55.05 delay 2 seconds @02:15:57.08 AS.reconfigure ASconfig @02:15:57.13 AS.seek 1ml @02:16:26.15 delay 1 second @02:16:27.18 AFV.select :holdingCoil @02:16:27.29 delay 1 second @02:16:28.31 ATV.dial :waste @02:16:29.95 AS.reconfigure ASnorm @02:16:30.00 AS.seek :empty @02:17:20.74 AFV.select :carrierSolution @02:17:20.86 delay 2 seconds @02:17:22.89 AS.reconfigure ASconfig @02:17:22.94 AS.seek 0.5ml @02:17:52.04 delay 1 second @02:17:53.06 AFV.select :holdingCoil @02:17:53.17 Building Slug @02:17:53.18 ATV.dial :ARV @02:17:54.78 delay 2 seconds @02:17:56.80 ARV.dial :air1 @02:17:57.21 delay 2 seconds @02:17:59.29 AS.reconfigure ASslow2 @02:17:59.35 AS.seek 0.506ml @02:18:05.72 delay 2 seconds @02:18:07.75 ARV.dial :MM2 @02:18:09.27 delay 2 seconds @02:18:11.30 Adding MM2 @02:18:11.37 AS.seek 0.524ml @02:18:29.76 delay 25 seconds @02:18:54.79 ARV.dial :PP4 @02:18:55.20 Adding PP4 @02:18:55.21 delay 2 seconds @02:18:57.30 AS.seek 0.53ml @02:19:03.82 delay 10 seconds @02:19:13.85 ARV.dial :air3 @02:19:14.25 adding ARV air @02:19:14.26 delay 2 seconds @02:19:16.35 AS.seek 0.536ml @02:19:22.60 delay 5 seconds @02:19:27.63 ARV.dial :carrier @02:19:28.92 delay 7 seconds @02:19:36.01 AS.seek 0.594ml @02:20:34.65 delay 5 seconds @02:20:39.68 ATV.dial :ASV @02:20:40.08 delay 2 seconds @02:20:42.17 AS.seek 0.6ml @02:20:48.54 delay 4 seconds @02:20:52.57 ATV.dial ARV @02:20:53.10 delay 2 seconds @02:20:55.12 ARV.dial :carrier @02:20:55.14 delay 2 seconds @02:20:57.22 AS.seek 0.65ml @02:21:47.69 delay 4 seconds @02:21:51.72 ATV.dial PCR @02:21:52.32 ASV.dial :waste1 @02:21:52.34 ARV.dial :air1 @02:21:52.76 Positioning Slug by 0.324ml @02:21:52.84 AS.seek 0.326ml @02:27:18.96 delay 5 seconds @02:27:23.99 ATV.dial ARV @02:27:24.76 ASV.dial :PCRlock @02:27:25.16 delay 5 seconds @02:27:30.18 Running Tt1 Profile60C300 thermocycling @02:27:30.20 PCR.program Profile60C300 @02:27:30.32 Profile60C300 should complete in < 1:45:21 @02:27:30.34 PCR.run 2:16:41.25 @03:52:13.83 PCR.off @03:52:15.41 PCR for Tt1 completed -- closed /var/log/esp/Tt118sep1702h200ml.pcr @03:52:15.43 Full MFB Decontamination @03:52:15.44 Decon extraction column and decon AIV @03:52:15.46 SPE.hold 2:46:40,55C @03:52:15.51 ASV.dial :SPEdelivery @03:52:16.17 delay 20 seconds @03:52:36.19 AFV.select :carrierSolution @03:52:36.30 delay 2 seconds @03:52:38.33 AS.reconfigure ASconfig @03:52:38.38 AS.seek 0.5ml @03:52:48.69 delay 1 second @03:52:49.71 AFV.select :holdingCoil @03:52:49.81 ATV.dial :air1 @03:52:51.71 AS.reconfigure ASslow1 @03:52:51.76 AS.seek 0.52ml @03:52:56.08 ATV.dial :ASV @03:52:57.85 ASV.dial :SPErecovery @03:52:58.55 AS.seek 0.62ml @03:53:18.91 ATV.dial :waste @03:53:20.92 AS.reconfigure ASnorm @03:53:20.97 AS.seek :empty @03:53:52.60 AFV.select :holdingCoil @03:53:52.62 ATV.dial :waste @03:53:53.19 AS.reconfigure ASconfig @03:53:53.26 AS.seek 0ml @03:53:53.57 AFV.select :carrierSolution @03:53:53.69 delay 2 seconds @03:53:55.72 AS.seek 0.5ml @03:54:24.71 delay 1 second @03:54:25.73 AFV.select :holdingCoil @03:54:25.83 ATV.dial :air3 @03:54:26.57 AS.reconfigure ASslow1 @03:54:26.62 AS.seek 0.52ml @03:54:30.92 ATV.dial :air3 @03:54:31.02 AS.seek 0.53ml @03:54:33.35 delay 2 seconds @03:54:35.38 ATV.dial :bleach @03:54:35.86 AS.seek 0.71ml @03:55:12.47 delay 3 seconds @03:55:15.50 ATV.dial :air3 @03:55:16.08 delay 3 seconds @03:55:19.17 AS.seek 0.735ml @03:55:24.51 delay 3 seconds @03:55:27.53 ATV.dial :bleach @03:55:28.18 AS.seek 0.72ml @03:55:31.63 delay 3 seconds @03:55:34.66 ATV.dial ASV @03:55:35.74 ASV.dial :SPErecovery @03:55:35.83 AS.seek 0.365ml @03:56:47.53 ATV.dial :waste @03:56:49.25 AS.reconfigure ASnorm @03:56:49.30 AS.seek :empty @03:57:08.06 ATV.dial :air3 @03:57:08.75 AS.reconfigure ASslow1 @03:57:08.80 AS.seek 0.009ml @03:57:11.13 delay 2 seconds @03:57:13.15 ATV.dial :bleach @03:57:13.61 AS.seek 0.579ml @03:59:08.49 delay 3 seconds @03:59:11.52 ATV.dial :air3 @03:59:12.09 delay 3 seconds @03:59:15.18 AS.seek 0.604ml @03:59:20.59 delay 3 seconds @03:59:23.61 ATV.dial :bleach @03:59:24.24 AS.seek 0.589ml @03:59:27.61 delay 3 seconds @03:59:30.64 ATV.dial :ASV @03:59:31.66 ASV.dial :wasteR @03:59:32.15 AS.seek 0ml @04:01:30.94 delay 1:00 @04:02:30.97 ATV.dial ASV @04:02:30.98 AFV.select :carrierSolution @04:02:31.10 delay 2 seconds @04:02:33.13 AS.reconfigure ASconfig @04:02:33.18 AS.seek 1ml @04:03:31.00 delay 1 second @04:03:32.02 AFV.select :holdingCoil @04:03:32.13 delay 1 second @04:03:33.15 ASV.dial :wasteR @04:03:33.17 AS.reconfigure ASslow1 @04:03:33.23 AS.seek 0ml @04:06:54.61 delay 10 seconds @04:07:04.64 ATV.dial :air3 @04:07:06.16 AS.seek 0.01ml @04:07:08.39 delay 2 seconds @04:07:10.41 ATV.dial :bleach @04:07:11.04 AS.seek 0.58ml @04:09:05.97 delay 3 seconds @04:09:09.00 ATV.dial :air3 @04:09:09.55 delay 3 seconds @04:09:12.65 AS.seek 0.605ml @04:09:17.97 delay 3 seconds @04:09:21.00 ATV.dial :bleach @04:09:21.63 AS.seek 0.59ml @04:09:24.88 delay 3 seconds @04:09:27.90 ATV.dial ASV @04:09:28.92 ASV.dial :SPEdelivery @04:09:29.29 AS.seek 0ml @04:11:28.22 delay 1:00 @04:12:28.25 AFV.select :carrierSolution @04:12:28.36 delay 2 seconds @04:12:30.38 AS.reconfigure ASconfig @04:12:30.43 AS.seek 1ml @04:13:28.20 delay 1 second @04:13:29.22 AFV.select :holdingCoil @04:13:29.33 delay 1 second @04:13:30.35 ATV.dial ASV @04:13:30.37 ASV.dial :SPEdelivery @04:13:30.38 AS.reconfigure ASslow1 @04:13:30.43 AS.seek 0ml @04:16:51.85 delay 10 seconds @04:17:01.88 ATV.dial :air2 @04:17:03.20 AS.reconfigure ASslow0 @04:17:03.33 AS.seek 0.8ml @04:18:24.06 delay 3 seconds @04:18:27.08 ATV.dial :ASV @04:18:28.05 ASV.dial :SPEdelivery @04:18:28.08 AS.reconfigure ASslow1 @04:18:28.13 AS.seek 0.03ml @04:21:03.36 delay 20 seconds @04:21:23.39 ATV.dial :waste,via: :air3 @04:21:25.12 AS.reconfigure ASconfig @04:21:25.17 AS.seek :empty @04:21:27.25 SPE.coast @04:21:27.34 Removing Bleach from Mixing Coil @04:21:27.35 AFV.select :carrierSolution @04:21:27.46 delay 2 seconds @04:21:29.49 AS.seek 0.5ml @04:21:58.58 delay 1 second @04:21:59.60 AFV.select :holdingCoil @04:21:59.70 ATV.dial :ASV @04:22:01.36 ASV.dial :SPErecovery @04:22:02.01 AS.reconfigure ASslow1 @04:22:02.07 AS.seek 0.73ml @04:22:48.61 ASV.dial :wasteD @04:22:49.21 AS.reconfigure ASnorm @04:22:49.27 AS.seek 0ml @04:23:26.38 AFV.select :carrierSolution @04:23:26.50 delay 2 seconds @04:23:28.53 AS.reconfigure ASconfig @04:23:28.58 AS.seek 0.5ml @04:23:57.60 delay 1 second @04:23:58.62 AFV.select :holdingCoil @04:23:58.72 ATV.dial ASV @04:23:58.74 ASV.dial :SPErecovery @04:23:59.38 AS.reconfigure ASslow0 @04:23:59.44 AS.seek 0.3ml @04:24:19.89 ATV.dial :air2 @04:24:20.92 AS.reconfigure ASslow1 @04:24:20.97 AS.seek 0.32ml @04:24:25.31 ATV.dial ASV @04:24:26.46 AS.reconfigure ASslow0 @04:24:26.51 AS.seek 0.57ml @04:24:52.00 ASV.dial :wasteD @04:24:52.59 AS.reconfigure ASnorm @04:24:52.64 AS.seek 0ml @04:25:21.72 AFV.select :carrierSolution @04:25:21.84 delay 2 seconds @04:25:23.87 AS.reconfigure ASconfig @04:25:23.92 AS.seek 0.5ml @04:25:52.99 delay 1 second @04:25:54.01 AFV.select :holdingCoil @04:25:54.11 ATV.dial ASV @04:25:54.13 ASV.dial :SPErecovery @04:25:54.78 AS.reconfigure ASslow0 @04:25:54.83 AS.seek 0.3ml @04:26:15.30 ATV.dial :air2 @04:26:16.32 AS.reconfigure ASslow1 @04:26:16.38 AS.seek 0.32ml @04:26:20.70 ATV.dial ASV @04:26:21.74 AS.reconfigure ASslow0 @04:26:21.79 AS.seek 0.57ml @04:26:47.28 ASV.dial :wasteD @04:26:47.96 AS.reconfigure ASnorm @04:26:48.01 AS.seek 0ml @04:27:17.08 ATV.dial :air2 @04:27:18.04 AS.reconfigure ASslow0 @04:27:18.09 AS.seek 0.3ml @04:27:48.63 ATV.dial :ASV @04:27:49.60 ASV.dial :wasteD @04:27:49.63 AS.reconfigure ASconfig @04:27:49.67 AS.seek 0.05ml @04:28:04.36 ATV.dial :waste @04:28:06.18 AS.seek 0ml @04:28:09.37 ASV.dial :SPEdelivery @04:28:10.34 deconLysateINT @04:28:10.35 ATV.dial :air3 @04:28:11.01 AS.reconfigure ASslow1 @04:28:11.07 AS.seek 0.01ml @04:28:13.42 delay 2 seconds @04:28:15.44 ATV.dial :bleach @04:28:15.89 AS.seek 0.96ml @04:31:27.28 delay 3 seconds @04:31:30.31 ATV.dial :air3 @04:31:30.86 delay 3 seconds @04:31:33.95 AS.seek 0.985ml @04:31:39.29 delay 3 seconds @04:31:42.31 ATV.dial :bleach @04:31:43.58 AS.seek 0.97ml @04:31:46.89 delay 3 seconds @04:31:49.92 ATV.dial AIV @04:31:51.25 AIV.dial :coilToMFB @04:31:51.26 AWV.select :waste @04:31:51.27 AFV.select :carrierSolution @04:31:51.39 delay 2 seconds @04:31:53.42 AS.reconfigure ASconfig @04:31:53.47 AS.seek 1ml @04:31:55.55 delay 1 second @04:31:56.57 AFV.select :holdingCoil @04:31:56.68 delay 1 second @04:31:57.71 AS.reconfigure ASslow0 @04:31:57.75 AS.seek :empty @04:33:38.70 AFV.select :carrierSolution @04:33:38.82 delay 2 seconds @04:33:40.85 AS.reconfigure ASconfig @04:33:40.90 AS.seek 1ml @04:34:38.69 delay 1 second @04:34:39.72 AFV.select :holdingCoil @04:34:39.83 delay 1 second @04:34:40.86 AS.reconfigure ASslow0 @04:34:40.90 AS.seek :empty @04:36:21.89 AFV.select :carrierSolution @04:36:22.00 delay 2 seconds @04:36:24.04 AS.reconfigure ASconfig @04:36:24.09 AS.seek 1ml @04:37:21.90 delay 1 second @04:37:22.92 AFV.select :holdingCoil @04:37:23.03 delay 1 second @04:37:24.06 AS.reconfigure ASslow0 @04:37:24.10 AS.seek :empty @04:39:05.09 ATV.dial :air2 @04:39:05.76 AS.reconfigure ASslow1 @04:39:05.82 AS.seek 0.009ml @04:39:08.12 AS.reconfigure ASslow0 @04:39:08.17 AS.seek 1ml @04:40:48.10 ATV.dial AIV @04:40:48.85 AS.seek 0.02ml @04:42:27.77 ATV.dial :waste @04:42:29.40 AS.seek 0ml @04:42:31.75 AWV.select :air @04:42:31.92 Cleaning PCR @04:42:31.93 AFV.select :carrierSolution @04:42:32.04 delay 2 seconds @04:42:34.07 AS.reconfigure ASconfig @04:42:34.12 AS.seek 0.5ml @04:43:03.16 delay 1 second @04:43:04.18 AFV.select :holdingCoil @04:43:04.28 ASV.dial :waste1 @04:43:04.66 ATV.dial :air3 @04:43:05.34 AS.reconfigure ASslow1 @04:43:05.40 AS.seek 0.51ml @04:43:07.67 delay 2 seconds @04:43:09.69 ATV.dial :bleach @04:43:10.14 AS.seek 0.81ml @04:44:10.70 delay 3 seconds @04:44:13.72 ATV.dial :air3 @04:44:14.28 delay 3 seconds @04:44:17.36 AS.seek 0.835ml @04:44:22.69 delay 3 seconds @04:44:25.71 ATV.dial :bleach @04:44:26.35 AS.seek 0.82ml @04:44:29.64 delay 3 seconds @04:44:32.67 ARV.dial :waste @04:44:33.29 delay 3 seconds @04:44:36.31 ATV.dial :ARV @04:44:37.18 AS.reconfigure ASconfig @04:44:37.24 AS.seek 0.47ml @04:44:57.64 AFV.select :carrierSolution @04:44:57.76 delay 2 seconds @04:44:59.79 AS.seek 1ml @04:45:30.63 delay 1 second @04:45:31.65 AFV.select :holdingCoil @04:45:31.76 delay 1 second @04:45:32.79 AS.reconfigure ASfast @04:45:32.84 AS.seek 0ml @04:46:00.20 AFV.select :carrierSolution @04:46:00.32 delay 2 seconds @04:46:02.35 AS.reconfigure ASconfig @04:46:02.40 AS.seek 1ml @04:47:00.17 delay 1 second @04:47:01.19 AFV.select :holdingCoil @04:47:01.30 delay 1 second @04:47:02.33 AS.reconfigure ASfast @04:47:02.38 AS.seek 0ml @04:47:29.74 AFV.select :carrierSolution @04:47:29.86 delay 2 seconds @04:47:31.89 AS.reconfigure ASconfig @04:47:31.94 AS.seek 1ml @04:48:29.78 delay 1 second @04:48:30.80 AFV.select :holdingCoil @04:48:30.91 delay 1 second @04:48:31.94 AS.reconfigure ASfast @04:48:31.99 AS.seek 0ml @04:48:59.36 ARV.dial between carrier and air1 @04:48:59.87 ATV.dial :air3 @04:49:01.13 AS.reconfigure ASslow1 @04:49:01.19 AS.seek 0.01ml @04:49:03.50 delay 2 seconds @04:49:05.52 ATV.dial :bleach @04:49:06.82 AS.seek 0.81ml @04:51:47.97 delay 3 seconds @04:51:51.00 ATV.dial :air3 @04:51:51.53 delay 3 seconds @04:51:54.63 AS.seek 0.835ml @04:52:00.02 delay 3 seconds @04:52:03.04 ATV.dial :bleach @04:52:03.67 AS.seek 0.82ml @04:52:07.06 delay 3 seconds @04:52:10.09 ATV.dial PCR @04:52:10.48 AS.reconfigure ASconfig @04:52:10.53 AS.seek :empty @04:52:58.00 PCR.program :bleachProfile @04:52:58.05 PCR program should complete in < 2:45 @04:52:58.07 PCR.run 10:00 @04:56:05.87 PCR.off @04:56:05.90 AFV.select :carrierSolution @04:56:06.02 delay 2 seconds @04:56:08.05 AS.seek 1ml @04:57:05.84 delay 1 second @04:57:06.86 AFV.select :holdingCoil @04:57:06.97 delay 1 second @04:57:08.00 AS.reconfigure ASfast @04:57:08.05 AS.seek 0ml @04:57:35.39 delay 3 seconds @04:57:38.41 AFV.select :carrierSolution @04:57:38.52 delay 2 seconds @04:57:40.55 AS.reconfigure ASconfig @04:57:40.60 AS.seek 1ml @04:58:38.42 delay 1 second @04:58:39.44 AFV.select :holdingCoil @04:58:39.55 delay 1 second @04:58:40.58 AS.reconfigure ASfast @04:58:40.63 AS.seek 0ml @04:59:08.00 delay 3 seconds @04:59:11.03 AFV.select :carrierSolution @04:59:11.14 delay 2 seconds @04:59:13.17 AS.reconfigure ASconfig @04:59:13.23 AS.seek 1ml @05:00:11.00 delay 1 second @05:00:12.02 AFV.select :holdingCoil @05:00:12.13 delay 1 second @05:00:13.16 AS.reconfigure ASfast @05:00:13.22 AS.seek 0ml @05:00:40.56 delay 3 seconds @05:00:43.59 AFV.select :carrierSolution @05:00:43.70 delay 2 seconds @05:00:45.73 AS.reconfigure ASconfig @05:00:45.78 AS.seek 1ml @05:01:43.56 delay 1 second @05:01:44.58 AFV.select :holdingCoil @05:01:44.69 delay 1 second @05:01:45.72 AS.reconfigure ASfast @05:01:45.77 AS.seek 0ml @05:02:13.13 delay 3 seconds @05:02:16.16 AFV.select :carrierSolution @05:02:16.27 delay 2 seconds @05:02:18.30 AS.reconfigure ASconfig @05:02:18.41 AS.seek 1ml @05:03:16.14 delay 1 second @05:03:17.16 AFV.select :holdingCoil @05:03:17.27 delay 1 second @05:03:18.30 AS.reconfigure ASnorm @05:03:18.34 AS.seek 0ml @05:04:09.06 AFV.select :carrierSolution @05:04:09.18 delay 2 seconds @05:04:11.21 AS.reconfigure ASconfig @05:04:11.26 AS.seek 1ml @05:05:09.03 delay 1 second @05:05:10.05 AFV.select :holdingCoil @05:05:10.16 delay 1 second @05:05:11.18 ATV.dial :waste @05:05:12.39 AS.reconfigure ASnorm @05:05:12.44 AS.seek 0.5ml @05:05:38.00 ATV.dial :waste2 @05:05:39.24 AS.seek 0ml @05:06:04.77
Asleep after completing 18tun1 assays @05:06:04.79 GoodNews.email "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22}\nCan@05:03:29, 22.9C, 43% humidity, 14.7psia, 12.046V, 0.544A, 0.544A avg, 6.55W",Subject: "Asleep after completing 18tun1 assays" @05:06:04.81
Mission runtime was 10:32:40.16194 @17:56:13.18CEST01-Oct-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @17:56:15.22 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @17:56:15.51 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @17:56:20.78 Polling Can every 10:00 @17:56:20.83 Loading /home/esp/esp2/mission/18tundeployv2.rb mission @17:56:20.91 Preparing to sleep until +0:00:10 @17:56:20.93 GoodNews.email "Starting 18tundeployv2 w/pucks in tube 2\n{}\nCan@17:56:20, 16.4C, 65% humidity, 14.5psia, 15.505V, 0.184A, 0.184A avg, 2.86W",Subject: "18tundeployv2 begun" @17:56:21.01 Shutdown @17:56:21.03
Asleep til +0:00:10 @17:56:21.05 Continuing at 17:56 @17:56:30.86 Powering core bus @17:56:35.79 Gate.power :raw,:ON @17:56:36.47 SPE.coast @17:56:36.53 SPE.configure SPEconfig @17:56:36.63 AS.configure ASconfig @17:56:36.74 SS.configure SSconfig @17:56:36.84 CC.configure CCconfig @17:56:36.94 CS.configure CSconfig @17:56:37.04 PC.configure PCconfig @17:56:37.14 PS.configure PSconfig @17:56:37.27 Forearm.configure LooseForearm @17:56:37.36 Elbow.configure LooseElbow @17:56:37.49 SC.configure SCloose @17:56:37.58 SE.configure SEconfig @17:56:37.63 CH.coast @17:56:37.69 CH.configure CHconfig @17:56:37.72 PH.coast @17:56:37.79 PH.configure PHconfig @17:56:37.83 PCR.off @17:56:37.86 PCR.pidtemp= 50,0.1,0,300,25,25 @17:56:37.89 PCR.caltemp= 2.7073,0.0373,0 @17:56:37.92 All dwarf controllers running firmware version 3.75 @17:56:38.05 Valve.reset @17:56:38.22 PCR.off @17:56:38.25 PCR.pidtemp= 50,0.1,0,300,25,25 @17:56:38.28 PCR.caltemp= 2.7073,0.0373,0 @17:56:38.52 PSV.dial :air @17:56:38.54 Intake.select :close @17:56:38.57 Exhaust.select :close @17:56:38.60 CTV.dial :air @17:56:38.77 PS.reconfigure PSconfig @17:56:38.86 PS.home.seek 0ml @17:56:38.90 CSV.dial :waste1 @17:56:38.96
Forearm.reconfigure LooseForearm @17:56:39.08 Forearm.home.seek :retract @17:56:39.09 SSV.dial :spit,avoiding: :exhaust @17:56:39.30 CS.reconfigure CSfast @17:56:39.33 SS.reconfigure SSconfig @17:56:39.43 CS.home.seek 0ml @17:56:39.46 SS.home.seek 0ml @17:56:39.84 Hand.close @17:56:39.87
Elbow.jog 1500 @17:56:40.42 Elbow.reconfigure LooseElbow @17:56:40.47 Elbow.home.seek :clear @17:56:45.03 SE.reconfigure SEconfig @17:56:45.08 SE.home.seek :down @17:56:45.76 SC.reconfigure SCsloppy @17:56:45.81 SC.home.seek :initial @17:56:47.91 PS.coast @17:56:49.73
Forearm.seek :retract @17:56:49.81 Elbow.seek :hammer @17:56:50.95 Forearm.reconfigure TightForearm @17:56:51.00 Forearm.seek 5 + 350 counts @17:56:52.08 Forearm.seek :retract @17:56:53.29 PC.reconfigure PCconfig @17:56:53.31
CC.reconfigure CCconfig @17:56:53.44 PC.home.seek :open @17:56:53.46
CC.home.seek :open @17:56:56.80 Phase [mbaPhase] @17:56:56.82CEST01-Oct-18 ==================> STARTED Superphase (phase #1) <=== @17:56:56.84 Starting initialPurge @17:56:56.86 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:56:56.99 Hand.open @17:56:57.77 Elbow.reconfigure TightElbow @17:56:57.82 Elbow.seek FlushPuck::Garage @17:56:59.70 Elbow.reconfigure LooseElbow @17:56:59.81 Forearm.reconfigure LooseForearm @17:56:59.86 Forearm.seek :garage @17:57:00.87 Hand.close @17:57:01.65 Forearm.seek :retract @17:57:02.68 Elbow.reconfigure TightElbow @17:57:02.73 Elbow.seek CC @17:57:05.68 Elbow.reconfigure LooseElbow @17:57:05.79 Forearm.reconfigure TightForearm @17:57:05.84 Forearm.seek CC @17:57:07.15 CC.seek :closed @17:57:11.42 Collection Clamp onSpring/offSpring for 6/32 samples, on/off torque 1.60 @17:57:11.50 Hand.open @17:57:12.21 Forearm.reconfigure LooseForearm @17:57:12.26 Forearm.seek :retract @17:57:13.23 Flushing the Collection Valve::Manifold @17:57:13.37 CSV.dial CSR @17:57:14.62 Pulling 5ml of CSR.flush @17:57:14.63 CSR.select :flush @17:57:14.76 CS.reconfigure CSconfig @17:57:14.81 CS.seek 5ml @17:57:28.53 delay 5 seconds @17:57:33.55 CSR.select :air @17:57:33.73 CS.reconfigure CSfast @17:57:33.78 CS.seek 7.5ml @17:57:39.99 CSV.dial :waste @17:57:41.18 CS.reconfigure CSconfig @17:57:41.23 CS.seek 0ml @17:58:01.40 Purging external Intake @17:58:01.53 CSV.dial :flush @17:58:01.98 CS.reconfigure CSfast @17:58:02.03 CS.seek 10ml @17:58:23.03 delay 5 seconds @17:58:28.06 CSV.dial CBV @17:58:28.09
CBV.dial CSV @17:58:28.82 CTV.dial :intake @17:58:29.50 Intake.select :open @17:58:29.62 CS.seek 0ml @17:58:51.30 Intake.select :close @17:58:51.42 CTV.dial :air @17:58:52.14 Flushing upper manifold @17:58:52.27 CSV.dial :flush @17:58:52.96 CS.seek 5ml @17:59:04.06 delay 5 seconds @17:59:09.08 CSV.dial :air @17:59:09.60 CS.seek 7ml @17:59:14.73 CSV.dial CBV @17:59:14.75
CBV.dial CSV @17:59:15.72 CTV.dial :air @17:59:15.74 CS.seek 0ml @17:59:24.51 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/18tundeployv2.rb:106\n{}\nCan@17:56:21, 16.5C, 65% humidity, 14.5psia, 15.495V, 0.247A, 0.247A avg, 3.83W",Subject: "Asleep until 17:56" @17:59:30.75
delay 7 seconds @17:59:31.91 GoodNews.email "At 17:56:56 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tundeployv2.rb:106\n{}\nCan@17:56:56, 16.4C, 65% humidity, 14.4psia, 15.367V, 0.651A, 0.657A avg, 10.00W",Subject: "Started 18tundeployv2 phase mbaPhase" @17:59:37.80
CS.seek 3ml @17:59:45.03 CSV.dial :waste @17:59:45.52 CS.seek 0ml @17:59:52.75 CSV.dial :flush @17:59:53.24 CS.seek 3ml @18:00:00.32 delay 5 seconds @18:00:05.34 CSV.dial :air @18:00:05.96 CS.seek 5ml @18:00:11.23 CSV.dial :air @18:00:11.26 CS.seek 0ml @18:00:22.31 delay 7 seconds @18:00:29.35 CS.seek 3ml @18:00:36.43 CSV.dial :waste @18:00:37.15 CS.seek 0ml @18:00:44.25 Garaging FlushPuck in Collection Clamp @18:00:44.33 Elbow.reconfigure TightElbow @18:00:44.38 Elbow.seek CC @18:00:44.62 Elbow.reconfigure LooseElbow @18:00:44.73 Forearm.seek CC @18:00:45.71 Hand.close @18:00:46.39 CC.seek :open @18:00:50.72 Forearm.seek :retract @18:00:51.70 Elbow.reconfigure TightElbow @18:00:51.75 Elbow.seek :garage @18:00:54.87 Elbow.reconfigure LooseElbow @18:00:54.98 Forearm.reconfigure TightForearm @18:00:55.03 Forearm.seek :garage @18:00:56.19 Hand.open @18:00:56.84 Forearm.reconfigure LooseForearm @18:00:56.88 Forearm.seek :retract @18:00:57.97 Starting mfb @18:00:57.99 pcrBAC sampling at most 1500ml, wcr at most 1500ml @18:00:58.03 BACfans.email "Started mfb in tube 2 at 18:00:58CEST01-Oct-18\n{}\nCan@17:56:56, 16.4C, 65% humidity, 14.4psia, 15.367V, 0.651A, 0.657A avg, 10.00W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @18:00:58.12
Loading sh1 puck from tube 2 into Collection Clamp @18:00:58.21 Readying the MFB @18:00:58.28 Homing MFB Syringe @18:00:58.29 AFV.select :holdingCoil @18:00:58.54 ATV.dial :air2 @18:00:58.67
SC.reconfigure SCloose @18:00:58.91 Elbow.reconfigure TightElbow @18:00:58.96 Elbow.seek 2 @18:01:00.07 AS.reconfigure ASconfig @18:01:00.14 AS.home.seek :home @18:01:01.00
Forearm.reconfigure TightForearm @18:01:01.05 Forearm.seek 2 + 700 counts @18:01:02.12 SE.reconfigure SEpick @18:01:02.19 SE.seek beamBreak @18:01:03.48 SE.configure SEconfig @18:01:03.66 Elbow.seek 2 @18:01:03.87 Forearm.seek 2 + 300 counts @18:01:04.12 Forearm.reconfigure LooseForearm @18:01:04.17 Hand.close @18:01:05.08 SE.seek :down @18:01:05.33
Forearm.seek :retract @18:01:05.72 AFV.select :carrierSolution @18:01:05.92 delay 2 seconds @18:01:06.31 SE.coast @18:01:06.39
Elbow.seek CC @18:01:07.97 AS.seek 1ml @18:01:08.15
Elbow.reconfigure LooseElbow @18:01:08.29 Forearm.reconfigure TightForearm @18:01:08.34 Forearm.seek CC @18:01:09.65 CC.seek :closed @18:01:13.90 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.65 @18:01:13.91 Hand.open @18:01:14.59 Forearm.reconfigure LooseForearm @18:01:14.64 Forearm.seek :retract @18:01:15.63 SC.coast @18:01:15.67 collectSample 1500ml w/bubble point of 55psi @18:01:15.68 Priming 7ml @18:01:15.69 Intake.select :close @18:01:15.70 Exhaust.select :close @18:01:15.71 SS.hold @18:01:15.99 Calibrating pressure and temperature sensors @18:01:16.01 CTV.dial :air @18:01:16.02 SSV.dial :spit,avoiding: :exhaust @18:01:16.05 SS.reconfigure SSfast @18:01:16.10 SS.seek 5ml @18:01:18.66 SSV.dial CBV @18:01:18.68
CBV.dial SSV @18:01:19.93 delay 3.8 seconds @18:01:23.88 SSV.dial :spit @18:01:25.12 SS.seek 0ml @18:01:27.89 SSV.dial CBV @18:01:29.12 CTV.dial :intake @18:01:29.83 SSV.dial CBV @18:01:29.86
CBV.dial SSV @18:01:29.87 Allowing 40s for 7.0ml of remaining 7.0ml @18:01:29.89 Intake.select :open @18:01:32.06 Intake @34.5psia, vacuum from 20.0 to 24.0psi @18:01:32.08 SS.reconfigure SSinhale.with maxSpeed: 150 @18:01:32.13 SS.seek 7ml @18:01:35.13 Vacuum too high -- waiting up to 40s @18:01:36.75 Intake @34.7psia, vacuum from 20.0 to 24.0psi @18:01:36.77 SS.reconfigure SSinhale.with maxSpeed: 135 @18:01:36.82 SS.seek 7ml @18:01:39.35 Vacuum too high -- waiting up to 40s @18:01:40.73 Intake @35.1psia, vacuum from 20.0 to 24.0psi @18:01:40.75 SS.reconfigure SSinhale.with maxSpeed: 122 @18:01:40.80 SS.seek 7ml @18:01:43.26 Vacuum too high -- waiting up to 40s @18:01:44.60 Intake @34.7psia, vacuum from 20.0 to 24.0psi @18:01:44.62 SS.reconfigure SSinhale.with maxSpeed: 110 @18:01:44.67 SS.seek 7ml @18:01:44.91 Stroke completed -- waiting for pressure to equalize ... @18:01:46.27 Intake.select :close @18:01:46.43 SSV.dial :exhaust @18:01:47.14 Exhaust.select :open @18:01:47.25 SS.reconfigure SSexhale @18:01:47.30 SS.seek 0ml @18:01:51.05 Exhaust.select :close @18:01:51.17 Sampling 1500.0ml @18:01:51.18 Intake.select :close @18:01:51.19 Exhaust.select :close @18:01:51.21 SS.reconfigure SSconfig @18:01:51.25 SS.hold @18:01:51.47 CTV.dial :intake @18:01:51.49 SSV.dial CBV @18:01:51.52
CBV.dial SSV @18:01:52.28 Allowing 130s for 25.0ml of remaining 1500.0ml @18:01:52.29 Intake.select :open @18:01:54.47 Intake @33.9psia, vacuum from 20.0 to 24.0psi @18:01:54.48 SS.reconfigure SSinhale.with maxSpeed: 110 @18:01:54.53 SS.seek 25ml @18:01:57.38 Vacuum too high -- waiting up to 40s @18:01:58.75 Intake @34.9psia, vacuum from 20.0 to 24.0psi @18:01:58.77 SS.reconfigure SSinhale.with maxSpeed: 99 @18:01:58.82 SS.seek 25ml @18:02:00.97 delay 1 second @18:02:01.20
Vacuum too high -- waiting up to 40s @18:02:02.00 AFV.select :holdingCoil @18:02:02.11 delay 1 second @18:02:02.31
Intake @34.6psia, vacuum from 20.0 to 24.0psi @18:02:02.33 SS.reconfigure SSinhale.with maxSpeed: 89 @18:02:02.38 SS.seek 25ml @18:02:03.13 ATV.dial :waste @18:02:04.11 AS.reconfigure ASnorm @18:02:04.16 AS.home.seek :empty @18:02:04.76
Vacuum too high -- waiting up to 40s @18:02:05.86 Intake @34.9psia, vacuum from 20.0 to 24.0psi @18:02:05.87 SS.reconfigure SSinhale.with maxSpeed: 80 @18:02:05.92 SS.seek 25ml @18:02:08.48 Vacuum too high -- waiting up to 40s @18:02:09.34 Intake @34.6psia, vacuum from 20.0 to 24.0psi @18:02:09.36 SS.reconfigure SSinhale.with maxSpeed: 72 @18:02:09.41 SS.seek 25ml @18:02:11.85 Vacuum too high -- waiting up to 40s @18:02:12.91 SS.reconfigure SSinhale.with maxSpeed: 65 @18:02:13.01 SS.seek 25ml @18:02:16.35 Vacuum too high -- waiting up to 40s @18:02:17.21 Intake @34.7psia, vacuum from 20.0 to 24.0psi @18:02:17.23 SS.reconfigure SSinhale.with maxSpeed: 59 @18:02:17.29 SS.seek 25ml @18:02:20.94 Vacuum too high -- waiting up to 40s @18:02:21.78 Intake @34.4psia, vacuum from 20.0 to 24.0psi @18:02:21.80 SS.reconfigure SSinhale.with maxSpeed: 53 @18:02:21.85 SS.seek 25ml @18:02:28.04 Vacuum too high -- waiting up to 40s @18:02:28.89 Intake @34.8psia, vacuum from 20.0 to 24.0psi @18:02:28.91 SS.reconfigure SSinhale.with maxSpeed: 48 @18:02:28.96 SS.seek 25ml @18:02:36.92 Stroke completed -- waiting for pressure to equalize ... @18:02:39.41 Intake.select :close @18:02:39.57 SSV.dial :exhaust @18:02:40.29 Exhaust.select :open @18:02:40.40 SS.reconfigure SSexhale @18:02:40.45 SS.seek 0ml @18:02:49.53 Exhaust.select :close @18:02:49.65 SSV.dial CBV @18:02:49.68
CBV.dial SSV @18:02:50.43 Allowing 130s for 25.0ml of remaining 1475.0ml @18:02:50.44 Intake.select :open @18:02:52.62 Intake @33.9psia, vacuum from 20.0 to 24.0psi @18:02:52.63 SS.reconfigure SSinhale.with maxSpeed: 48 @18:02:52.69 SS.seek 25ml @18:02:56.69 ATV.dial :waste @18:02:56.71 ARV.dial between carrier and air1 @18:02:56.79 ASV.dial :waste1 @18:03:29.72
Stroke completed -- waiting for pressure to equalize ... @18:03:32.33 Intake.select :close @18:03:32.49 SSV.dial :exhaust @18:03:33.23 Exhaust.select :open @18:03:33.34 SS.reconfigure SSexhale @18:03:33.39 SS.seek 0ml @18:03:42.53 Exhaust.select :close @18:03:42.65 SSV.dial CBV @18:03:42.68
CBV.dial SSV @18:03:43.43 Allowing 130s for 25.0ml of remaining 1450.0ml @18:03:43.44 Intake.select :open @18:03:44.26 Session Begun @18:03:45.61
Intake @34.2psia, vacuum from 20.0 to 24.0psi @18:03:45.64 SS.reconfigure SSinhale.with maxSpeed: 48 @18:03:45.73 SS.seek 25ml @18:04:18.55 -> exit @18:04:18.57 Session Ended @18:04:22.49
Stroke completed -- waiting for pressure to equalize ... @18:04:25.09 Intake.select :close @18:04:25.24 SSV.dial :exhaust @18:04:26.00 Exhaust.select :open @18:04:26.11 SS.reconfigure SSexhale @18:04:26.16 SS.seek 0ml @18:04:35.13 Exhaust.select :close @18:04:35.25 SSV.dial CBV @18:04:35.30
CBV.dial SSV @18:04:36.04 Allowing 130s for 25.0ml of remaining 1425.0ml @18:04:36.05 Intake.select :open @18:04:38.22 Intake @33.8psia, vacuum from 20.0 to 24.0psi @18:04:38.24 SS.reconfigure SSinhale.with maxSpeed: 48 @18:04:38.29 SS.seek 25ml @18:05:15.50 Stroke completed -- waiting for pressure to equalize ... @18:05:18.08 Intake.select :close @18:05:18.24 SSV.dial :exhaust @18:05:18.98 Exhaust.select :open @18:05:19.09 SS.reconfigure SSexhale @18:05:19.14 SS.seek 0ml @18:05:27.85 Exhaust.select :close @18:05:27.97 SSV.dial CBV @18:05:28.00
CBV.dial SSV @18:05:28.76 Allowing 130s for 25.0ml of remaining 1400.0ml @18:05:28.77 Intake.select :open @18:05:30.94 Intake @33.9psia, vacuum from 20.0 to 24.0psi @18:05:30.96 SS.reconfigure SSinhale.with maxSpeed: 48 @18:05:31.01 SS.seek 25ml @18:06:08.41 Stroke completed -- waiting for pressure to equalize ... @18:06:11.00 Intake.select :close @18:06:11.16 SSV.dial :exhaust @18:06:11.89 Exhaust.select :open @18:06:12.00 SS.reconfigure SSexhale @18:06:12.05 SS.seek 0ml @18:06:20.81 Exhaust.select :close @18:06:20.93 SSV.dial CBV @18:06:20.96
CBV.dial SSV @18:06:21.71 Allowing 130s for 25.0ml of remaining 1375.0ml @18:06:21.72 Intake.select :open @18:06:23.89 Intake @33.6psia, vacuum from 20.0 to 24.0psi @18:06:23.91 SS.reconfigure SSinhale.with maxSpeed: 48 @18:06:23.96 SS.seek 25ml @18:07:01.31 Stroke completed -- waiting for pressure to equalize ... @18:07:04.40 Intake.select :close @18:07:04.56 SSV.dial :exhaust @18:07:05.29 Exhaust.select :open @18:07:05.40 SS.reconfigure SSexhale @18:07:05.45 SS.seek 0ml @18:07:14.69 Exhaust.select :close @18:07:14.81 SSV.dial CBV @18:07:14.84
CBV.dial SSV @18:07:15.60 Allowing 130s for 25.0ml of remaining 1350.0ml @18:07:15.61 Intake.select :open @18:07:17.79 Intake @33.7psia, vacuum from 20.0 to 24.0psi @18:07:17.80 SS.reconfigure SSinhale.with maxSpeed: 48 @18:07:17.85 SS.seek 25ml @18:07:54.99 Stroke completed -- waiting for pressure to equalize ... @18:07:57.61 Intake.select :close @18:07:57.76 SSV.dial :exhaust @18:07:58.48 Exhaust.select :open @18:07:58.59 SS.reconfigure SSexhale @18:07:58.64 SS.seek 0ml @18:08:07.78 Exhaust.select :close @18:08:07.90 SSV.dial CBV @18:08:07.93
CBV.dial SSV @18:08:08.68 Allowing 130s for 25.0ml of remaining 1325.0ml @18:08:08.69 Intake.select :open @18:08:10.86 Intake @33.8psia, vacuum from 20.0 to 24.0psi @18:08:10.88 SS.reconfigure SSinhale.with maxSpeed: 48 @18:08:10.93 SS.seek 25ml @18:08:48.06 Stroke completed -- waiting for pressure to equalize ... @18:08:50.67 Intake.select :close @18:08:50.83 SSV.dial :exhaust @18:08:51.56 Exhaust.select :open @18:08:51.68 SS.reconfigure SSexhale @18:08:51.73 SS.seek 0ml @18:09:00.89 Exhaust.select :close @18:09:01.01 SSV.dial CBV @18:09:01.04
CBV.dial SSV @18:09:01.78 Allowing 130s for 25.0ml of remaining 1300.0ml @18:09:01.79 Intake.select :open @18:09:03.97 Intake @33.6psia, vacuum from 20.0 to 24.0psi @18:09:03.98 SS.reconfigure SSinhale.with maxSpeed: 48 @18:09:04.03 SS.seek 25ml @18:09:41.20 Stroke completed -- waiting for pressure to equalize ... @18:09:44.42 Intake.select :close @18:09:44.58 SSV.dial :exhaust @18:09:45.31 Exhaust.select :open @18:09:45.43 SS.reconfigure SSexhale @18:09:45.48 SS.seek 0ml @18:09:54.23 Exhaust.select :close @18:09:54.35 SSV.dial CBV @18:09:54.38
CBV.dial SSV @18:09:55.12 Allowing 130s for 25.0ml of remaining 1275.0ml @18:09:55.13 Intake.select :open @18:09:57.30 Intake @34.1psia, vacuum from 20.0 to 24.0psi @18:09:57.32 SS.reconfigure SSinhale.with maxSpeed: 48 @18:09:57.37 SS.seek 25ml @18:10:29.04 Vacuum too high -- waiting up to 40s @18:10:29.87 Intake @33.7psia, vacuum from 20.0 to 24.0psi @18:10:29.89 SS.reconfigure SSinhale.with maxSpeed: 43 @18:10:29.94 SS.seek 25ml @18:10:36.58 Stroke completed -- waiting for pressure to equalize ... @18:10:39.17 Intake.select :close @18:10:39.33 SSV.dial :exhaust @18:10:40.07 Exhaust.select :open @18:10:40.18 SS.reconfigure SSexhale @18:10:40.23 SS.seek 0ml @18:10:49.23 Exhaust.select :close @18:10:49.35 SSV.dial CBV @18:10:49.38
CBV.dial SSV @18:10:50.12 Allowing 130s for 25.0ml of remaining 1250.0ml @18:10:50.13 Intake.select :open @18:10:52.30 Intake @33.8psia, vacuum from 20.0 to 24.0psi @18:10:52.32 SS.reconfigure SSinhale.with maxSpeed: 43 @18:10:52.37 SS.seek 25ml @18:11:33.63 Stroke completed -- waiting for pressure to equalize ... @18:11:36.71 Intake.select :close @18:11:36.87 SSV.dial :exhaust @18:11:37.62 Exhaust.select :open @18:11:37.73 SS.reconfigure SSexhale @18:11:37.78 SS.seek 0ml @18:11:46.89 Exhaust.select :close @18:11:47.01 SSV.dial CBV @18:11:47.04
CBV.dial SSV @18:11:47.78 Allowing 130s for 25.0ml of remaining 1225.0ml @18:11:47.79 Intake.select :open @18:11:49.97 Intake @34.3psia, vacuum from 20.0 to 24.0psi @18:11:49.98 SS.reconfigure SSinhale.with maxSpeed: 43 @18:11:50.03 SS.seek 25ml @18:12:31.36 Stroke completed -- waiting for pressure to equalize ... @18:12:33.96 Intake.select :close @18:12:34.12 SSV.dial :exhaust @18:12:34.87 Exhaust.select :open @18:12:34.98 SS.reconfigure SSexhale @18:12:35.03 SS.seek 0ml @18:12:44.11 Exhaust.select :close @18:12:44.23 SSV.dial CBV @18:12:44.26
CBV.dial SSV @18:12:45.03 Allowing 130s for 25.0ml of remaining 1200.0ml @18:12:45.04 Intake.select :open @18:12:47.21 Intake @33.9psia, vacuum from 20.0 to 24.0psi @18:12:47.23 SS.reconfigure SSinhale.with maxSpeed: 43 @18:12:47.28 SS.seek 25ml @18:13:28.64 Stroke completed -- waiting for pressure to equalize ... @18:13:31.25 Intake.select :close @18:13:31.40 SSV.dial :exhaust @18:13:32.14 Exhaust.select :open @18:13:32.25 SS.reconfigure SSexhale @18:13:32.30 SS.seek 0ml @18:13:41.11 Exhaust.select :close @18:13:41.23 SSV.dial CBV @18:13:41.26
CBV.dial SSV @18:13:41.99 Allowing 130s for 25.0ml of remaining 1175.0ml @18:13:42.00 Intake.select :open @18:13:44.17 Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:13:44.19 SS.reconfigure SSinhale.with maxSpeed: 43 @18:13:44.24 SS.seek 25ml @18:14:25.79 Stroke completed -- waiting for pressure to equalize ... @18:14:28.38 Intake.select :close @18:14:28.54 SSV.dial :exhaust @18:14:29.27 Exhaust.select :open @18:14:29.39 SS.reconfigure SSexhale @18:14:29.44 SS.seek 0ml @18:14:38.39 Exhaust.select :close @18:14:38.51 SSV.dial CBV @18:14:38.54
CBV.dial SSV @18:14:39.28 Allowing 130s for 25.0ml of remaining 1150.0ml @18:14:39.29 Intake.select :open @18:14:41.46 Intake @33.8psia, vacuum from 20.0 to 24.0psi @18:14:41.48 SS.reconfigure SSinhale.with maxSpeed: 43 @18:14:41.53 SS.seek 25ml @18:15:22.87 Stroke completed -- waiting for pressure to equalize ... @18:15:25.47 Intake.select :close @18:15:25.62 SSV.dial :exhaust @18:15:26.37 Exhaust.select :open @18:15:26.48 SS.reconfigure SSexhale @18:15:26.53 SS.seek 0ml @18:15:35.47 Exhaust.select :close @18:15:35.59 SSV.dial CBV @18:15:35.62
CBV.dial SSV @18:15:36.37 Allowing 130s for 25.0ml of remaining 1125.0ml @18:15:36.38 Intake.select :open @18:15:38.55 Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:15:38.57 SS.reconfigure SSinhale.with maxSpeed: 43 @18:15:38.62 SS.seek 25ml @18:16:19.89 Stroke completed -- waiting for pressure to equalize ... @18:16:23.11 Intake.select :close @18:16:23.67 SSV.dial :exhaust @18:16:24.50 Exhaust.select :open @18:16:24.62 SS.reconfigure SSexhale @18:16:24.67 SS.seek 0ml @18:16:33.58 Exhaust.select :close @18:16:33.70 SSV.dial CBV @18:16:33.73
CBV.dial SSV @18:16:34.48 Allowing 130s for 25.0ml of remaining 1100.0ml @18:16:34.49 Intake.select :open @18:16:36.67 Intake @33.9psia, vacuum from 20.0 to 24.0psi @18:16:36.68 SS.reconfigure SSinhale.with maxSpeed: 43 @18:16:36.73 SS.seek 25ml @18:17:17.91 Stroke completed -- waiting for pressure to equalize ... @18:17:21.14 Intake.select :close @18:17:21.30 SSV.dial :exhaust @18:17:22.03 Exhaust.select :open @18:17:22.15 SS.reconfigure SSexhale @18:17:22.20 SS.seek 0ml @18:17:31.20 Exhaust.select :close @18:17:31.32 SSV.dial CBV @18:17:31.35
CBV.dial SSV @18:17:32.12 Allowing 130s for 25.0ml of remaining 1075.0ml @18:17:32.13 Intake.select :open @18:17:34.30 Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:17:34.32 SS.reconfigure SSinhale.with maxSpeed: 43 @18:17:34.37 SS.seek 25ml @18:18:15.70 Stroke completed -- waiting for pressure to equalize ... @18:18:19.00 Intake.select :close @18:18:19.16 SSV.dial :exhaust @18:18:19.89 Exhaust.select :open @18:18:20.01 SS.reconfigure SSexhale @18:18:20.06 SS.seek 0ml @18:18:29.17 Exhaust.select :close @18:18:29.29 SSV.dial CBV @18:18:29.32
CBV.dial SSV @18:18:30.07 Allowing 130s for 25.0ml of remaining 1050.0ml @18:18:30.08 Intake.select :open @18:18:32.25 Intake @34.1psia, vacuum from 20.0 to 24.0psi @18:18:32.27 SS.reconfigure SSinhale.with maxSpeed: 43 @18:18:32.32 SS.seek 25ml @18:19:13.37 Stroke completed -- waiting for pressure to equalize ... @18:19:16.58 Intake.select :close @18:19:16.74 SSV.dial :exhaust @18:19:17.48 Exhaust.select :open @18:19:17.59 SS.reconfigure SSexhale @18:19:17.64 SS.seek 0ml @18:19:26.59 Exhaust.select :close @18:19:26.71 SSV.dial CBV @18:19:26.74
CBV.dial SSV @18:19:27.48 Allowing 130s for 25.0ml of remaining 1025.0ml @18:19:27.49 Intake.select :open @18:19:29.66 Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:19:29.68 SS.reconfigure SSinhale.with maxSpeed: 43 @18:19:29.73 SS.seek 25ml @18:20:11.03 Stroke completed -- waiting for pressure to equalize ... @18:20:14.24 Intake.select :close @18:20:14.39 SSV.dial :exhaust @18:20:15.13 Exhaust.select :open @18:20:15.24 SS.reconfigure SSexhale @18:20:15.29 SS.seek 0ml @18:20:24.22 Exhaust.select :close @18:20:24.34 SSV.dial CBV @18:20:24.86
CBV.dial SSV @18:20:25.59 Allowing 130s for 25.0ml of remaining 1000.0ml @18:20:25.60 Intake.select :open @18:20:27.78 Intake @34.4psia, vacuum from 20.0 to 24.0psi @18:20:27.79 SS.reconfigure SSinhale.with maxSpeed: 43 @18:20:27.84 SS.seek 25ml @18:20:53.07 Vacuum too high -- waiting up to 40s @18:20:54.18 Intake @34.0psia, vacuum from 20.0 to 24.0psi @18:20:54.20 SS.reconfigure SSinhale.with maxSpeed: 39 @18:20:54.25 SS.seek 25ml @18:21:12.26 Stroke completed -- waiting for pressure to equalize ... @18:21:15.48 Intake.select :close @18:21:15.64 SSV.dial :exhaust @18:21:16.37 Exhaust.select :open @18:21:16.49 SS.reconfigure SSexhale @18:21:16.54 SS.seek 0ml @18:21:25.58 Exhaust.select :close @18:21:25.70 SSV.dial CBV @18:21:25.73
CBV.dial SSV @18:21:26.51 Allowing 130s for 25.0ml of remaining 975.0ml @18:21:26.52 Intake.select :open @18:21:28.70 Intake @34.1psia, vacuum from 20.0 to 24.0psi @18:21:28.72 SS.reconfigure SSinhale.with maxSpeed: 39 @18:21:28.77 SS.seek 25ml @18:22:14.22 Stroke completed -- waiting for pressure to equalize ... @18:22:17.44 Intake.select :close @18:22:17.59 SSV.dial :exhaust @18:22:18.33 Exhaust.select :open @18:22:18.45 SS.reconfigure SSexhale @18:22:18.50 SS.seek 0ml @18:22:27.45 Exhaust.select :close @18:22:27.57 SSV.dial CBV @18:22:27.60
CBV.dial SSV @18:22:28.37 Allowing 130s for 25.0ml of remaining 950.0ml @18:22:28.38 Intake.select :open @18:22:30.55 Intake @34.1psia, vacuum from 20.0 to 24.0psi @18:22:30.57 SS.reconfigure SSinhale.with maxSpeed: 39 @18:22:30.62 SS.seek 25ml @18:23:15.89 Stroke completed -- waiting for pressure to equalize ... @18:23:19.11 Intake.select :close @18:23:19.26 SSV.dial :exhaust @18:23:20.01 Exhaust.select :open @18:23:20.12 SS.reconfigure SSexhale @18:23:20.17 SS.seek 0ml @18:23:28.94 Exhaust.select :close @18:23:29.06 SSV.dial CBV @18:23:29.09
CBV.dial SSV @18:23:29.85 Allowing 130s for 25.0ml of remaining 925.0ml @18:23:29.86 Intake.select :open @18:23:32.03 Intake @34.3psia, vacuum from 20.0 to 24.0psi @18:23:32.05 SS.reconfigure SSinhale.with maxSpeed: 39 @18:23:32.10 SS.seek 25ml @18:24:17.76 Stroke completed -- waiting for pressure to equalize ... @18:24:21.58 Intake.select :close @18:24:21.73 SSV.dial :exhaust @18:24:22.96 Exhaust.select :open @18:24:23.08 SS.reconfigure SSexhale @18:24:23.13 SS.seek 0ml @18:24:31.89 Exhaust.select :close @18:24:32.01 SSV.dial CBV @18:24:32.04
CBV.dial SSV @18:24:32.79 Allowing 130s for 25.0ml of remaining 900.0ml @18:24:32.80 Intake.select :open @18:24:34.97 Intake @34.4psia, vacuum from 20.0 to 24.0psi @18:24:34.99 SS.reconfigure SSinhale.with maxSpeed: 39 @18:24:35.04 SS.seek 25ml @18:25:20.79 Stroke completed -- waiting for pressure to equalize ... @18:25:24.66 Intake.select :close @18:25:24.82 SSV.dial :exhaust @18:25:25.55 Exhaust.select :open @18:25:25.66 SS.reconfigure SSexhale @18:25:25.71 SS.seek 0ml @18:25:34.87 Exhaust.select :close @18:25:34.99 SSV.dial CBV @18:25:35.02
CBV.dial SSV @18:25:35.76 Allowing 130s for 25.0ml of remaining 875.0ml @18:25:35.77 Intake.select :open @18:25:37.94 Intake @34.3psia, vacuum from 20.0 to 24.0psi @18:25:37.96 SS.reconfigure SSinhale.with maxSpeed: 39 @18:25:38.01 SS.seek 25ml @18:26:14.60 Vacuum too high -- waiting up to 40s @18:26:15.70 Intake @34.1psia, vacuum from 20.0 to 24.0psi @18:26:15.71 SS.reconfigure SSinhale.with maxSpeed: 35 @18:26:15.76 SS.seek 25ml @18:26:25.76 Stroke completed -- waiting for pressure to equalize ... @18:26:28.99 Intake.select :close @18:26:29.14 SSV.dial :exhaust @18:26:29.90 Exhaust.select :open @18:26:30.01 SS.reconfigure SSexhale @18:26:30.06 SS.seek 0ml @18:26:39.08 Exhaust.select :close @18:26:39.20 SSV.dial CBV @18:26:39.23
CBV.dial SSV @18:26:39.98 Allowing 130s for 25.0ml of remaining 850.0ml @18:26:39.99 Intake.select :open @18:26:42.16 Intake @34.4psia, vacuum from 20.0 to 24.0psi @18:26:42.18 SS.reconfigure SSinhale.with maxSpeed: 35 @18:26:42.23 SS.seek 25ml @18:27:32.92 Stroke completed -- waiting for pressure to equalize ... @18:27:36.15 Intake.select :close @18:27:36.30 SSV.dial :exhaust @18:27:37.04 Exhaust.select :open @18:27:37.16 SS.reconfigure SSexhale @18:27:37.21 SS.seek 0ml @18:27:46.00 Exhaust.select :close @18:27:46.12 SSV.dial CBV @18:27:46.15
CBV.dial SSV @18:27:46.88 Allowing 130s for 25.0ml of remaining 825.0ml @18:27:46.89 Intake.select :open @18:27:49.06 Intake @34.3psia, vacuum from 20.0 to 24.0psi @18:27:49.08 SS.reconfigure SSinhale.with maxSpeed: 35 @18:27:49.13 SS.seek 25ml @18:28:39.90 Stroke completed -- waiting for pressure to equalize ... @18:28:43.61 Intake.select :close @18:28:43.77 SSV.dial :exhaust @18:28:44.51 Exhaust.select :open @18:28:44.62 SS.reconfigure SSexhale @18:28:44.67 SS.seek 0ml @18:28:53.54 Exhaust.select :close @18:28:53.66 SSV.dial CBV @18:28:53.69
CBV.dial SSV @18:28:54.43 Allowing 130s for 25.0ml of remaining 800.0ml @18:28:54.44 Intake.select :open @18:28:56.62 Intake @34.1psia, vacuum from 20.0 to 24.0psi @18:28:56.63 SS.reconfigure SSinhale.with maxSpeed: 35 @18:28:56.68 SS.seek 25ml @18:29:47.43 Stroke completed -- waiting for pressure to equalize ... @18:29:51.29 Intake.select :close @18:29:51.45 SSV.dial :exhaust @18:29:52.19 Exhaust.select :open @18:29:52.30 SS.reconfigure SSexhale @18:29:52.35 SS.seek 0ml @18:30:01.42 Exhaust.select :close @18:30:01.54 SSV.dial CBV @18:30:01.57
CBV.dial SSV @18:30:02.30 Allowing 130s for 25.0ml of remaining 775.0ml @18:30:02.31 Intake.select :open @18:30:04.48 Intake @34.1psia, vacuum from 20.0 to 24.0psi @18:30:04.50 SS.reconfigure SSinhale.with maxSpeed: 35 @18:30:04.55 SS.seek 25ml @18:30:55.04 Stroke completed -- waiting for pressure to equalize ... @18:30:58.94 Intake.select :close @18:30:59.10 SSV.dial :exhaust @18:30:59.84 Exhaust.select :open @18:30:59.95 SS.reconfigure SSexhale @18:31:00.00 SS.seek 0ml @18:31:09.00 Exhaust.select :close @18:31:09.12 SSV.dial CBV @18:31:09.15
CBV.dial SSV @18:31:09.93 Allowing 130s for 25.0ml of remaining 750.0ml @18:31:09.94 Intake.select :open @18:31:12.11 Intake @34.1psia, vacuum from 20.0 to 24.0psi @18:31:12.13 SS.reconfigure SSinhale.with maxSpeed: 35 @18:31:12.18 SS.seek 25ml @18:32:02.43 Stroke completed -- waiting for pressure to equalize ... @18:32:06.90 Intake.select :close @18:32:07.05 SSV.dial :exhaust @18:32:07.81 Exhaust.select :open @18:32:07.92 SS.reconfigure SSexhale @18:32:07.97 SS.seek 0ml @18:32:16.89 Exhaust.select :close @18:32:17.01 SSV.dial CBV @18:32:17.04
CBV.dial SSV @18:32:17.79 Allowing 130s for 25.0ml of remaining 725.0ml @18:32:17.80 Intake.select :open @18:32:19.97 Intake @34.2psia, vacuum from 20.0 to 24.0psi @18:32:19.99 SS.reconfigure SSinhale.with maxSpeed: 35 @18:32:20.04 SS.seek 25ml @18:32:50.81 Vacuum too high -- waiting up to 40s @18:32:52.12 Intake @34.4psia, vacuum from 20.0 to 24.0psi @18:32:52.14 SS.reconfigure SSinhale.with maxSpeed: 32 @18:32:52.19 SS.seek 25ml @18:33:13.93 Stroke completed -- waiting for pressure to equalize ... @18:33:17.80 Intake.select :close @18:33:17.96 SSV.dial :exhaust @18:33:18.71 Exhaust.select :open @18:33:18.82 SS.reconfigure SSexhale @18:33:18.87 SS.seek 0ml @18:33:27.73 Exhaust.select :close @18:33:27.85 SSV.dial CBV @18:33:27.88
CBV.dial SSV @18:33:28.62 Allowing 130s for 25.0ml of remaining 700.0ml @18:33:28.63 Intake.select :open @18:33:30.80 Intake @34.3psia, vacuum from 20.0 to 24.0psi @18:33:30.82 SS.reconfigure SSinhale.with maxSpeed: 32 @18:33:30.87 SS.seek 25ml @18:34:26.20 Stroke completed -- waiting for pressure to equalize ... @18:34:30.68 Intake.select :close @18:34:30.84 SSV.dial :exhaust @18:34:31.59 Exhaust.select :open @18:34:31.70 SS.reconfigure SSexhale @18:34:31.75 SS.seek 0ml @18:34:40.37 Exhaust.select :close @18:34:40.49 SSV.dial CBV @18:34:40.52
CBV.dial SSV @18:34:41.26 Allowing 130s for 25.0ml of remaining 675.0ml @18:34:41.27 Intake.select :open @18:34:43.44 Intake @34.3psia, vacuum from 20.0 to 24.0psi @18:34:43.46 SS.reconfigure SSinhale.with maxSpeed: 32 @18:34:43.51 SS.seek 25ml @18:35:38.90 Stroke completed -- waiting for pressure to equalize ... @18:35:44.00 Intake.select :close @18:35:44.16 SSV.dial :exhaust @18:35:44.90 Exhaust.select :open @18:35:45.01 SS.reconfigure SSexhale @18:35:45.06 SS.seek 0ml @18:35:54.14 Exhaust.select :close @18:35:54.26 SSV.dial CBV @18:35:54.29
CBV.dial SSV @18:35:55.04 Allowing 130s for 25.0ml of remaining 650.0ml @18:35:55.05 Intake.select :open @18:35:57.22 Intake @34.3psia, vacuum from 20.0 to 24.0psi @18:35:57.24 SS.reconfigure SSinhale.with maxSpeed: 32 @18:35:57.29 SS.seek 25ml @18:36:37.94 Vacuum too high -- waiting up to 40s @18:36:39.28 Intake @34.4psia, vacuum from 20.0 to 24.0psi @18:36:39.30 SS.reconfigure SSinhale.with maxSpeed: 29 @18:36:39.35 SS.seek 25ml @18:36:55.43 Stroke completed -- waiting for pressure to equalize ... @18:36:59.77 Intake.select :close @18:36:59.93 SSV.dial :exhaust @18:37:00.69 Exhaust.select :open @18:37:00.80 SS.reconfigure SSexhale @18:37:00.85 SS.seek 0ml @18:37:09.59 Exhaust.select :close @18:37:09.71 SSV.dial CBV @18:37:09.74
CBV.dial SSV @18:37:10.48 Allowing 130s for 25.0ml of remaining 625.0ml @18:37:10.49 Intake.select :open @18:37:12.67 Intake @34.2psia, vacuum from 20.0 to 24.0psi @18:37:12.68 SS.reconfigure SSinhale.with maxSpeed: 29 @18:37:12.73 SS.seek 25ml @18:38:13.67 Stroke completed -- waiting for pressure to equalize ... @18:38:18.16 Intake.select :close @18:38:18.32 SSV.dial :exhaust @18:38:19.07 Exhaust.select :open @18:38:19.18 SS.reconfigure SSexhale @18:38:19.23 SS.seek 0ml @18:38:28.21 Exhaust.select :close @18:38:28.33 SSV.dial CBV @18:38:28.36
CBV.dial SSV @18:38:29.10 Allowing 130s for 25.0ml of remaining 600.0ml @18:38:29.11 Intake.select :open @18:38:31.29 Intake @34.2psia, vacuum from 20.0 to 24.0psi @18:38:31.30 SS.reconfigure SSinhale.with maxSpeed: 29 @18:38:31.35 SS.seek 25ml @18:39:31.75 Stroke completed -- waiting for pressure to equalize ... @18:39:36.89 Intake.select :close @18:39:37.05 SSV.dial :exhaust @18:39:37.79 Exhaust.select :open @18:39:37.90 SS.reconfigure SSexhale @18:39:37.95 SS.seek 0ml @18:39:46.70 Exhaust.select :close @18:39:46.82 SSV.dial CBV @18:39:46.85
CBV.dial SSV @18:39:47.58 Allowing 130s for 25.0ml of remaining 575.0ml @18:39:47.59 Intake.select :open @18:39:49.76 Intake @34.3psia, vacuum from 20.0 to 24.0psi @18:39:49.78 SS.reconfigure SSinhale.with maxSpeed: 29 @18:39:49.83 SS.seek 25ml @18:40:37.35 Vacuum too high -- waiting up to 40s @18:40:38.95 Intake @34.4psia, vacuum from 20.0 to 24.0psi @18:40:38.98 SS.reconfigure SSinhale.with maxSpeed: 26 @18:40:39.07 SS.seek 25ml @18:40:54.40 Stroke completed -- waiting for pressure to equalize ... @18:40:58.73 Intake.select :close @18:40:58.89 SSV.dial :exhaust @18:40:59.64 Exhaust.select :open @18:40:59.75 SS.reconfigure SSexhale @18:40:59.80 SS.seek 0ml @18:41:08.62 Exhaust.select :close @18:41:08.74 SSV.dial CBV @18:41:08.77
CBV.dial SSV @18:41:09.51 Allowing 130s for 25.0ml of remaining 550.0ml @18:41:09.52 Intake.select :open @18:41:11.69 Intake @34.6psia, vacuum from 20.0 to 24.0psi @18:41:11.71 SS.reconfigure SSinhale.with maxSpeed: 26 @18:41:11.76 SS.seek 25ml @18:42:19.53 Stroke completed -- waiting for pressure to equalize ... @18:42:24.68 Intake.select :close @18:42:24.83 SSV.dial :exhaust @18:42:25.58 Exhaust.select :open @18:42:25.69 SS.reconfigure SSexhale @18:42:25.74 SS.seek 0ml @18:42:34.67 Exhaust.select :close @18:42:34.79 SSV.dial CBV @18:42:34.82
CBV.dial SSV @18:42:35.59 Allowing 130s for 25.0ml of remaining 525.0ml @18:42:35.62 Intake.select :open @18:42:37.82 Intake @34.4psia, vacuum from 20.0 to 24.0psi @18:42:37.83 SS.reconfigure SSinhale.with maxSpeed: 26 @18:42:37.88 SS.seek 25ml @18:43:45.73 Stroke completed -- waiting for pressure to equalize ... @18:43:51.48 Intake.select :close @18:43:51.63 SSV.dial :exhaust @18:43:52.37 Exhaust.select :open @18:43:52.49 SS.reconfigure SSexhale @18:43:52.54 SS.seek 0ml @18:44:01.56 Exhaust.select :close @18:44:01.68 SSV.dial CBV @18:44:01.71
CBV.dial SSV @18:44:02.44 Allowing 130s for 25.0ml of remaining 500.0ml @18:44:02.45 Intake.select :open @18:44:04.62 Intake @33.9psia, vacuum from 20.0 to 24.0psi @18:44:04.64 SS.reconfigure SSinhale.with maxSpeed: 26 @18:44:04.69 SS.seek 25ml @18:45:12.28 Stroke completed -- waiting for pressure to equalize ... @18:45:18.65 Intake.select :close @18:45:18.80 SSV.dial :exhaust @18:45:19.54 Exhaust.select :open @18:45:19.65 SS.reconfigure SSexhale @18:45:19.70 SS.seek 0ml @18:45:29.00 Exhaust.select :close @18:45:29.12 SSV.dial CBV @18:45:29.15
CBV.dial SSV @18:45:29.90 Allowing 130s for 25.0ml of remaining 475.0ml @18:45:29.91 Intake.select :open @18:45:32.09 Intake @34.6psia, vacuum from 20.0 to 24.0psi @18:45:32.11 SS.reconfigure SSinhale.with maxSpeed: 26 @18:45:32.16 SS.seek 25ml @18:46:15.38 Vacuum too high -- waiting up to 40s @18:46:16.99 Intake @34.5psia, vacuum from 20.0 to 24.0psi @18:46:17.01 SS.reconfigure SSinhale.with maxSpeed: 23 @18:46:17.06 SS.seek 25ml @18:46:44.95 Stroke completed -- waiting for pressure to equalize ... @18:46:50.09 Intake.select :close @18:46:50.25 SSV.dial :exhaust @18:46:50.99 Exhaust.select :open @18:46:51.10 SS.reconfigure SSexhale @18:46:51.15 SS.seek 0ml @18:47:00.34 Exhaust.select :close @18:47:00.46 SSV.dial CBV @18:47:00.49
CBV.dial SSV @18:47:01.22 Allowing 130s for 25.0ml of remaining 450.0ml @18:47:01.23 Intake.select :open @18:47:03.40 Intake @34.3psia, vacuum from 20.0 to 24.0psi @18:47:03.42 SS.reconfigure SSinhale.with maxSpeed: 23 @18:47:03.47 SS.seek 25ml @18:48:19.74 Stroke completed -- waiting for pressure to equalize ... @18:48:25.45 Intake.select :close @18:48:25.60 SSV.dial :exhaust @18:48:26.35 Exhaust.select :open @18:48:26.46 SS.reconfigure SSexhale @18:48:26.52 SS.seek 0ml @18:48:35.67 Exhaust.select :close @18:48:35.79 SSV.dial CBV @18:48:35.82
CBV.dial SSV @18:48:36.55 Allowing 130s for 25.0ml of remaining 425.0ml @18:48:36.56 Intake.select :open @18:48:38.73 Intake @34.9psia, vacuum from 20.0 to 24.0psi @18:48:38.75 SS.reconfigure SSinhale.with maxSpeed: 23 @18:48:38.80 SS.seek 25ml @18:49:54.99 Stroke completed -- waiting for pressure to equalize ... @18:50:01.36 Intake.select :close @18:50:02.00 SSV.dial :exhaust @18:50:02.75 Exhaust.select :open @18:50:02.86 SS.reconfigure SSexhale @18:50:02.91 SS.seek 0ml @18:50:11.79 Exhaust.select :close @18:50:11.91 SSV.dial CBV @18:50:11.94
CBV.dial SSV @18:50:12.68 Allowing 130s for 25.0ml of remaining 400.0ml @18:50:12.69 Intake.select :open @18:50:14.86 Intake @34.3psia, vacuum from 20.0 to 24.0psi @18:50:14.88 SS.reconfigure SSinhale.with maxSpeed: 23 @18:50:14.93 SS.seek 25ml @18:51:31.17 Stroke completed -- waiting for pressure to equalize ... @18:51:38.20 Intake.select :close @18:51:38.36 SSV.dial :exhaust @18:51:39.10 Exhaust.select :open @18:51:39.21 SS.reconfigure SSexhale @18:51:39.26 SS.seek 0ml @18:51:48.15 Exhaust.select :close @18:51:48.27 SSV.dial CBV @18:51:48.30
CBV.dial SSV @18:51:49.04 Allowing 130s for 25.0ml of remaining 375.0ml @18:51:49.05 Intake.select :open @18:51:51.25 Intake @34.4psia, vacuum from 20.0 to 24.0psi @18:51:51.27 SS.reconfigure SSinhale.with maxSpeed: 23 @18:51:51.32 SS.seek 25ml @18:52:45.25 Vacuum too high -- waiting up to 40s @18:52:47.08 Intake @34.1psia, vacuum from 20.0 to 24.0psi @18:52:47.09 SS.reconfigure SSinhale.with maxSpeed: 21 @18:52:47.14 SS.seek 25ml @18:53:12.18 Stroke completed -- waiting for pressure to equalize ... @18:53:18.54 Intake.select :close @18:53:18.69 SSV.dial :exhaust @18:53:19.43 Exhaust.select :open @18:53:19.55 SS.reconfigure SSexhale @18:53:19.61 SS.seek 0ml @18:53:28.43 Exhaust.select :close @18:53:28.54 SSV.dial CBV @18:53:28.57
CBV.dial SSV @18:53:29.30 Allowing 130s for 25.0ml of remaining 350.0ml @18:53:29.31 Intake.select :open @18:53:31.48 Intake @34.7psia, vacuum from 20.0 to 24.0psi @18:53:31.49 SS.reconfigure SSinhale.with maxSpeed: 21 @18:53:31.54 SS.seek 25ml @18:54:55.28 Stroke completed -- waiting for pressure to equalize ... @18:55:02.13 Intake.select :close @18:55:02.28 SSV.dial :exhaust @18:55:03.03 Exhaust.select :open @18:55:03.15 SS.reconfigure SSexhale @18:55:03.21 SS.seek 0ml @18:55:12.24 Exhaust.select :close @18:55:12.36 SSV.dial CBV @18:55:12.39
CBV.dial SSV @18:55:13.15 Allowing 130s for 25.0ml of remaining 325.0ml @18:55:13.16 Intake.select :open @18:55:15.33 Intake @34.3psia, vacuum from 20.0 to 24.0psi @18:55:15.35 SS.reconfigure SSinhale.with maxSpeed: 21 @18:55:15.40 SS.seek 25ml @18:56:25.50 Vacuum too high -- waiting up to 40s @18:56:27.57 Intake @34.2psia, vacuum from 20.0 to 24.0psi @18:56:27.58 SS.reconfigure SSinhale.with maxSpeed: 19 @18:56:27.64 SS.seek 25ml @18:56:42.90 Stroke completed -- waiting for pressure to equalize ... @18:56:49.23 Intake.select :close @18:56:49.38 SSV.dial :exhaust @18:56:50.12 Exhaust.select :open @18:56:50.24 SS.reconfigure SSexhale @18:56:50.29 SS.seek 0ml @18:56:59.32 Exhaust.select :close @18:56:59.44 SSV.dial CBV @18:56:59.47
CBV.dial SSV @18:57:00.21 Allowing 130s for 25.0ml of remaining 300.0ml @18:57:00.22 Intake.select :open @18:57:02.39 Intake @34.4psia, vacuum from 20.0 to 24.0psi @18:57:02.41 SS.reconfigure SSinhale.with maxSpeed: 19 @18:57:02.45 SS.seek 25ml @18:58:34.74 Stroke completed -- waiting for pressure to equalize ... @18:58:42.27 Intake.select :close @18:58:42.42 SSV.dial :exhaust @18:58:43.65 Exhaust.select :open @18:58:43.77 SS.reconfigure SSexhale @18:58:43.82 SS.seek 0ml @18:58:52.65 Exhaust.select :close @18:58:52.77 SSV.dial CBV @18:58:52.80
CBV.dial SSV @18:58:53.53 Allowing 130s for 25.0ml of remaining 275.0ml @18:58:53.54 Intake.select :open @18:58:55.72 Intake @34.4psia, vacuum from 20.0 to 24.0psi @18:58:55.73 SS.reconfigure SSinhale.with maxSpeed: 19 @18:58:55.79 SS.seek 25ml @19:00:28.43 Stroke completed -- waiting for pressure to equalize ... @19:00:36.63 Intake.select :close @19:00:36.78 SSV.dial :exhaust @19:00:37.52 Exhaust.select :open @19:00:37.64 SS.reconfigure SSexhale @19:00:37.70 SS.seek 0ml @19:00:46.87 Exhaust.select :close @19:00:46.99 SSV.dial CBV @19:00:47.02
CBV.dial SSV @19:00:47.75 Allowing 130s for 25.0ml of remaining 250.0ml @19:00:47.76 Intake.select :open @19:00:49.93 Intake @34.2psia, vacuum from 20.0 to 24.0psi @19:00:49.95 SS.reconfigure SSinhale.with maxSpeed: 19 @19:00:49.99 SS.seek 25ml @19:02:03.84 Vacuum too high -- waiting up to 40s @19:02:06.16 Intake @34.5psia, vacuum from 20.0 to 24.0psi @19:02:06.18 SS.reconfigure SSinhale.with maxSpeed: 17 @19:02:06.23 SS.seek 25ml @19:02:27.01 Stroke completed -- waiting for pressure to equalize ... @19:02:33.94 Intake.select :close @19:02:34.09 SSV.dial :exhaust @19:02:34.84 Exhaust.select :open @19:02:34.96 SS.reconfigure SSexhale @19:02:35.02 SS.seek 0ml @19:02:44.20 Exhaust.select :close @19:02:44.32 SSV.dial CBV @19:02:44.35
CBV.dial SSV @19:02:45.10 Allowing 130s for 25.0ml of remaining 225.0ml @19:02:45.11 Intake.select :open @19:02:47.28 Intake @34.4psia, vacuum from 20.0 to 24.0psi @19:02:47.30 SS.reconfigure SSinhale.with maxSpeed: 17 @19:02:47.35 SS.seek 25ml @19:04:31.23 Stroke completed -- waiting for pressure to equalize ... @19:04:39.44 Intake.select :close @19:04:39.59 SSV.dial :exhaust @19:04:40.34 Exhaust.select :open @19:04:40.46 SS.reconfigure SSexhale @19:04:40.52 SS.seek 0ml @19:04:49.46 Exhaust.select :close @19:04:49.58 SSV.dial CBV @19:04:49.61
CBV.dial SSV @19:04:50.35 Allowing 130s for 25.0ml of remaining 200.0ml @19:04:50.36 Intake.select :open @19:04:52.53 Intake @34.7psia, vacuum from 20.0 to 24.0psi @19:04:52.55 SS.reconfigure SSinhale.with maxSpeed: 17 @19:04:52.60 SS.seek 25ml @19:06:31.44 Vacuum too high -- waiting up to 40s @19:06:33.76 Intake @34.5psia, vacuum from 20.0 to 24.0psi @19:06:33.78 SS.reconfigure SSinhale.with maxSpeed: 15 @19:06:33.83 SS.seek 25ml @19:06:39.45 Stroke completed -- waiting for pressure to equalize ... @19:06:46.39 Intake.select :close @19:06:46.54 SSV.dial :exhaust @19:06:47.27 Exhaust.select :open @19:06:47.39 SS.reconfigure SSexhale @19:06:47.45 SS.seek 0ml @19:06:56.30 Exhaust.select :close @19:06:56.42 SSV.dial CBV @19:06:56.46
CBV.dial SSV @19:06:57.17 Allowing 130s for 25.0ml of remaining 175.0ml @19:06:57.18 Intake.select :open @19:06:59.35 Intake @34.3psia, vacuum from 20.0 to 24.0psi @19:06:59.36 SS.reconfigure SSinhale.with maxSpeed: 15 @19:06:59.41 SS.seek 25ml @19:08:56.52 Stroke completed -- waiting for pressure to equalize ... @19:09:04.61 Intake.select :close @19:09:04.76 SSV.dial :exhaust @19:09:05.49 Exhaust.select :open @19:09:05.61 SS.reconfigure SSexhale @19:09:05.67 SS.seek 0ml @19:09:14.71 Exhaust.select :close @19:09:14.83 SSV.dial CBV @19:09:14.86
CBV.dial SSV @19:09:15.60 Allowing 130s for 25.0ml of remaining 150.0ml @19:09:15.61 Intake.select :open @19:09:17.82 Intake @34.9psia, vacuum from 20.0 to 24.0psi @19:09:17.83 SS.reconfigure SSinhale.with maxSpeed: 15 @19:09:17.88 SS.seek 25ml @19:11:14.60 Stroke completed -- waiting for pressure to equalize ... @19:11:23.40 Intake.select :close @19:11:23.55 SSV.dial :exhaust @19:11:24.29 Exhaust.select :open @19:11:24.41 SS.reconfigure SSexhale @19:11:24.47 SS.seek 0ml @19:11:33.43 Exhaust.select :close @19:11:33.55 SSV.dial CBV @19:11:33.60
CBV.dial SSV @19:11:34.30 Allowing 130s for 25.0ml of remaining 125.0ml @19:11:34.31 Intake.select :open @19:11:36.48 Intake @34.4psia, vacuum from 20.0 to 24.0psi @19:11:36.50 SS.reconfigure SSinhale.with maxSpeed: 15 @19:11:36.55 SS.seek 25ml @19:13:33.87 Stroke completed -- waiting for pressure to equalize ... @19:13:43.87 Intake.select :close @19:13:44.02 SSV.dial :exhaust @19:13:44.77 Exhaust.select :open @19:13:44.89 SS.reconfigure SSexhale @19:13:44.94 SS.seek 0ml @19:13:53.82 Exhaust.select :close @19:13:53.94 SSV.dial CBV @19:13:54.43
CBV.dial SSV @19:13:55.20 Allowing 130s for 25.0ml of remaining 100.0ml @19:13:55.21 Intake.select :open @19:13:57.38 Intake @34.6psia, vacuum from 20.0 to 24.0psi @19:13:57.40 SS.reconfigure SSinhale.with maxSpeed: 15 @19:13:57.45 SS.seek 25ml @19:15:00.97 Vacuum too high -- waiting up to 40s @19:15:03.29 Intake @34.6psia, vacuum from 20.0 to 24.0psi @19:15:03.31 SS.reconfigure SSinhale.with maxSpeed: 14 @19:15:03.36 SS.seek 25ml @19:16:01.58 Stroke completed -- waiting for pressure to equalize ... @19:16:05.22 SS.reconfigure SSconfig @19:16:05.27 SS.hold @19:16:05.40 Intake.select :close @19:16:05.57 ShallowSampler::Clogged in MAIN -- progress too slow after 1425.0ml @19:16:05.58 SSV.dial :exhaust @19:16:06.32 Exhaust.select :open @19:16:06.44 SS.reconfigure SSexhale @19:16:06.49 SS.seek 0ml @19:16:15.21 Exhaust.select :close @19:16:15.33 Sampled 1425.0ml @19:16:15.35 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @19:16:15.42 CSV.dial :waste @19:16:15.47 CS.seek 0ml @19:16:15.58 SSV.dial CBV,avoiding: :exhaust @19:16:15.62
CBV.dial SSV @19:16:15.67 CSV.dial :air @19:16:16.37 CS.seek 10ml @19:16:17.35
CTV.dial :air @19:16:18.06 delay 15 seconds @19:16:33.08 SSV.dial :spit,avoiding: :exhaust @19:16:34.31 CTV.dial CSV,avoiding: :intake @19:16:34.35
SS.seek 0ml @19:16:34.50 SSV.dial CBV,avoiding: :exhaust @19:16:37.71 CBV.dial between SSV and waste2 @19:16:38.12 CTV.dial CSV,avoiding: :air @19:16:38.15
CSV.dial CTV @19:16:38.59 CS.reconfigure CSpressurize @19:16:38.64 CS.seek 0ml @19:17:04.67 BubblePoint: Intake @71.4psia, Outlet @15.8psia @19:17:04.68 CBV.dial SSV @19:17:05.03 SS.reconfigure SSconfig @19:17:05.08 SS.seek 10ml @19:17:10.45 delay 15 seconds @19:17:25.59 Evacuated: Intake @36.8psia, Outlet @2.2psia @19:17:25.60 SSV.dial :spit,avoiding: :exhaust @19:17:26.83 SS.reconfigure SSexhale @19:17:26.88 SS.seek 0ml @19:17:31.68 SS.reconfigure SSfast @19:17:31.73 SS.seek 5ml @19:17:34.38 SSV.dial CBV,avoiding: :exhaust @19:17:34.41
CBV.dial SSV @19:17:35.64 CTV.dial :air,avoiding: :intake @19:17:37.35 CSV.dial CTV @19:17:37.40
SS.seek 10ml @19:17:38.39 CSV.dial :air @19:17:38.85 CS.reconfigure CSfast @19:17:38.90 CS.seek 0ml @19:17:39.55 CS.coast @19:17:39.80
SSV.dial :spit,avoiding: :exhaust @19:17:41.05 SS.reconfigure SSexhale @19:17:41.10 SS.seek 0ml @19:17:46.17 SS.reconfigure SSfast @19:17:46.22 SS.seek 5ml @19:17:48.70 SSV.dial CBV,avoiding: :exhaust @19:17:48.73
CBV.dial SSV @19:17:51.00 SSV.dial :spit,avoiding: :exhaust @19:17:52.25 SS.reconfigure SSconfig @19:17:52.30 SS.seek 0ml @19:17:55.41 SS.coast @19:17:55.46 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @19:17:55.47 Intake.select :close @19:17:55.55 CSV.dial :waste @19:17:55.67 SSV.dial CBV,avoiding: :exhaust @19:17:55.70
CBV.dial SSV @19:17:56.31 CS.seek 0ml @19:17:56.40 CSV.dial :air @19:17:56.95
CTV.dial :air @19:17:56.96 delay 15 seconds @19:17:57.10 CS.seek 10ml @19:18:11.99
SSV.dial :spit,avoiding: :exhaust @19:18:13.22 CTV.dial CSV,avoiding: :intake @19:18:13.27
SS.reconfigure SSexhale @19:18:13.36 SS.seek 0ml @19:18:13.71 SSV.dial CBV,avoiding: :exhaust @19:18:18.34 CBV.dial between SSV and waste2 @19:18:18.74 CSV.dial between air and CTV @19:18:19.07 CS.seek 7.892ml @19:18:24.49 CTV.dial CSV,avoiding: :air @19:18:24.52
CSV.dial CTV @19:18:24.82 CS.reconfigure CSpressurize @19:18:24.87 CS.seek 0ml @19:18:45.70 CSV.dial :air @19:18:46.15 CS.reconfigure CSfast @19:18:46.20 CS.seek 10ml @19:19:07.21 CSV.dial between air and CTV @19:19:07.55 CS.seek 3.068ml @19:19:22.53 CTV.dial CSV,avoiding: :air @19:19:22.56
CSV.dial CTV @19:19:22.85 CS.reconfigure CSpressurize @19:19:22.90 CS.seek 0ml @19:19:30.79 BubblePoint: Intake @70.9psia, Outlet @15.7psia @19:19:30.80 CBV.dial SSV @19:19:31.14 SS.reconfigure SSconfig @19:19:31.19 SS.seek 10ml @19:19:36.66 delay 15 seconds @19:19:51.79 Evacuated: Intake @40.8psia, Outlet @16.5psia @19:19:51.80 SSV.dial :spit,avoiding: :exhaust @19:19:53.03 SS.reconfigure SSexhale @19:19:53.08 SS.seek 0ml @19:19:58.17 SS.reconfigure SSfast @19:19:58.22 SS.seek 5ml @19:20:00.69 SSV.dial CBV,avoiding: :exhaust @19:20:01.22
CBV.dial SSV @19:20:02.47 CTV.dial :air,avoiding: :intake @19:20:03.70 CSV.dial CTV @19:20:03.75
SS.seek 10ml @19:20:04.74 CSV.dial :air @19:20:05.20 CS.reconfigure CSfast @19:20:05.25 CS.seek 0ml @19:20:06.16
SSV.dial :spit,avoiding: :exhaust @19:20:06.71 CS.coast @19:20:07.39
SS.reconfigure SSexhale @19:20:07.44 SS.seek 0ml @19:20:12.49 SS.reconfigure SSfast @19:20:12.54 SS.seek 5ml @19:20:15.03 SSV.dial CBV,avoiding: :exhaust @19:20:15.06
CBV.dial SSV @19:20:17.34 SSV.dial :spit,avoiding: :exhaust @19:20:18.58 SS.reconfigure SSconfig @19:20:18.63 SS.seek 0ml @19:20:21.74 SS.coast @19:20:21.78 Lysing: Adding 1.5ml lysis @19:20:21.80 CBV.dial between CSV and waste1,avoiding: :DBV @19:20:23.15 CTV.dial CSV @19:20:24.36 CSV.dial CSR @19:20:25.12 Pulling 1ml of CSR.lysis @19:20:25.13 CSR.select :lysis @19:20:25.24 CS.reconfigure CSconfig @19:20:25.30 CS.seek 1ml @19:20:28.88 delay 5 seconds @19:20:33.90 CSR.select :air @19:20:34.08 CS.seek 6ml @19:20:47.87 delay 2 seconds @19:20:49.88 CSV.dial :waste1 @19:20:51.07 CS.reconfigure CSfast @19:20:51.12 CS.seek :empty @19:21:04.17 CSV.dial CSR @19:21:05.43 Pulling 1.5ml of CSR.lysis @19:21:05.44 CSR.select :lysis @19:21:05.55 CS.reconfigure CSconfig @19:21:05.61 CS.seek 1.5ml @19:21:10.50 delay 5 seconds @19:21:15.52 CSR.select :air @19:21:15.70 CS.seek 4.5ml @19:21:24.42 delay 2 seconds @19:21:26.44 CSV.dial CTV @19:21:26.89 CS.seek 1.275ml @19:21:36.15 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @19:21:36.17 CH.seek 85C,8:00 @19:23:52.63 CH.hold 5:00,85C @19:28:52.80 Collection Puck Heater at 85C after lysing @19:28:52.82 CS.seek 0ml @19:28:57.10 CTV.dial between spare and air,avoiding: :intake @19:28:58.07 CSV.dial :air @19:28:58.50 CS.seek 5ml @19:29:12.21 CSV.dial CTV @19:29:12.66 delay 2 seconds @19:29:14.67 CSV.dial :air @19:29:15.13 delay 2 seconds @19:29:17.14 CSV.dial CTV @19:29:17.61 delay 2 seconds @19:29:19.63 CSV.dial :air @19:29:20.61 delay 2 seconds @19:29:22.63 CS.seek 0ml @19:29:36.32 Recovering Lysate into Collection syringe @19:29:36.33 CSV.dial CBV @19:29:37.29 CBV.dial CSV @19:29:37.68 CS.seek 5ml @19:29:51.44 delay 5 seconds @19:29:56.46 CTV.dial :air @19:29:56.98 CS.seek 7ml @19:30:03.13 delay 5 seconds @19:30:08.14 CSV.dial :air2 @19:30:08.58 CS.seek 10ml @19:30:17.32 CSV.dial between air and CTV,avoiding: CBV @19:30:18.38 Lysing Complete @19:30:18.46 Storing sh1 puck in Collection Clamp to tube 1 @19:30:18.65 SC.reconfigure SCtight @19:30:18.69 SC.seek 1 @19:30:20.85 SC.reconfigure SCloose @19:30:21.07 Elbow.reconfigure TightElbow @19:30:21.12 Elbow.seek 1 @19:30:23.09 Forearm.reconfigure TightForearm @19:30:23.14 Forearm.seek 1 + 700 counts @19:30:24.16 SE.reconfigure SEpick @19:30:24.23 SE.seek beamBreak @19:30:27.02 SE.configure SEconfig @19:30:27.20 Elbow.reconfigure LimpElbow @19:30:27.25 Forearm.reconfigure LooseForearm @19:30:27.29 Forearm.seek 1 @19:30:27.60 Elbow.hold @19:30:27.67 Elbow.reconfigure TightElbow @19:30:27.77 SE.seek 2.07 Pucks below Top Plate @19:30:28.25 Forearm.force -20 @19:30:28.29 Forearm.seek 1 + 332 counts @19:30:28.61 Forearm.reconfigure TightForearm @19:30:28.66 Forearm.seek 1 + 700 counts @19:30:28.90 SE.seek 3.07 Pucks below Top Plate @19:30:29.52 Forearm.reconfigure LooseForearm @19:30:29.57 Forearm.seek :retract @19:30:30.51 Elbow.seek CC @19:30:32.23 Elbow.reconfigure LooseElbow @19:30:32.34 Forearm.seek CC @19:30:33.31 Hand.close @19:30:33.88 CC.seek :open @19:30:38.36 Forearm.seek :retract @19:30:39.31 Elbow.reconfigure TightElbow @19:30:39.36 Elbow.seek 1 @19:30:41.19 Elbow.reconfigure LimpElbow @19:30:41.24 Forearm.seek 1 @19:30:42.27 Elbow.hold @19:30:42.85 Elbow.reconfigure TightElbow @19:30:42.90 Hand.open @19:30:43.64 Forearm.force -20 @19:30:43.68 Forearm.seek 1 + 349 counts @19:30:44.01 Forearm.reconfigure TightForearm @19:30:44.05 Forearm.seek 1 + 700 counts @19:30:44.46 SE.seek :down @19:30:45.61
Forearm.reconfigure LooseForearm @19:30:45.65 Forearm.seek :retract @19:30:47.03 SE.coast @19:30:47.07
SC.coast @19:30:47.11 Tranferring lysate to PRV @19:30:47.12 CSV.dial :air @19:30:47.43 CS.seek 10ml @19:30:47.51 CSV.dial between air and CTV @19:30:47.83 AIV.dial :bypass @19:30:48.02 Beginning samplePassC @19:30:48.03 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:30:48.11 Hand.open @19:30:48.53 Elbow.seek FlushPuck::Garage @19:30:50.46 Elbow.reconfigure LooseElbow @19:30:50.57 Forearm.seek :garage @19:30:51.56 Hand.close @19:30:52.26 Forearm.seek :retract @19:30:53.26 Elbow.reconfigure TightElbow @19:30:53.31 Elbow.seek CC @19:30:56.24 Elbow.reconfigure LooseElbow @19:30:56.34 Forearm.reconfigure TightForearm @19:30:56.39 Forearm.seek CC @19:30:57.64 CC.seek :closed @19:31:01.95 Collection Clamp onSpring/offSpring for 6/32 samples, on/off torque 1.68 @19:31:02.04 Hand.open @19:31:02.69 Forearm.reconfigure LooseForearm @19:31:02.74 Forearm.seek :retract @19:31:03.70 AWV.select :air @19:31:03.72 AIV.dial :bypass @19:31:03.73 PSV.dial PSR @19:31:04.98 Pulling 1.5ml of PSR.flush @19:31:04.99 PSR.select :flush @19:31:05.12 PS.seek 1.5ml @19:31:21.13 delay 5 seconds @19:31:26.15 PSV.dial :waste1 @19:31:26.61 PS.seek 0ml @19:31:42.68 PSV.dial PSR @19:31:43.12 PSR.select :air @19:31:43.25 PS.seek 2.5ml @19:32:09.38 PRV.dial CBV @19:32:09.42
CBV.dial PRV @19:32:10.69 PSV.dial :mixloop @19:32:11.67 CTV.dial :air,avoiding: :intake @19:32:11.68 PTV.dial :PRVmixing @19:32:11.77 PS.seek 0ml @19:32:37.93 PSV.dial :air @19:32:38.38 PS.seek 2.5ml @19:33:04.59 PSV.dial :mixloop @19:33:05.05 PS.seek 0ml @19:33:31.15 PSV.dial :air @19:33:31.60 PS.seek 2.5ml @19:33:57.68 PSV.dial :mixloop @19:33:58.13 PS.seek 0ml @19:34:24.32 Garaging FlushPuck in Collection Clamp @19:34:24.40 Elbow.reconfigure TightElbow @19:34:24.45 Elbow.seek CC @19:34:24.71 Elbow.reconfigure LooseElbow @19:34:24.81 Forearm.seek CC @19:34:25.79 Hand.close @19:34:26.39 CC.seek :open @19:34:30.73 Forearm.seek :retract @19:34:31.73 Elbow.reconfigure TightElbow @19:34:31.78 Elbow.seek :garage @19:34:34.87 Elbow.reconfigure LooseElbow @19:34:34.97 Forearm.reconfigure TightForearm @19:34:35.02 Forearm.seek :garage @19:34:36.18 Hand.open @19:34:36.83 Forearm.reconfigure LooseForearm @19:34:36.87 Forearm.seek :retract @19:34:37.90 Connecting PS to CS to transfer sample @19:34:37.91 CBV.dial AIV @19:34:37.92 AIV.dial :bypass @19:34:37.93 PSV.dial :mixloop @19:34:37.94 PTV.dial :PRVmixing @19:34:37.96 PRV.dial AIV @19:34:37.97 CTV.dial :air,avoiding: :intake @19:34:37.99 Loading lyfil puck from tube 2 into Collection Clamp @19:34:38.24 SC.reconfigure SCtight @19:34:38.29 SC.seek 2 @19:34:40.54 SC.reconfigure SCloose @19:34:40.77 Elbow.reconfigure TightElbow @19:34:40.82 Elbow.seek 2 @19:34:42.97 Forearm.reconfigure TightForearm @19:34:43.02 Forearm.seek 2 + 700 counts @19:34:44.06 SE.reconfigure SEpick @19:34:44.13 SE.seek beamBreak @19:34:45.51 SE.configure SEconfig @19:34:45.68 Elbow.seek 2 @19:34:45.93 Forearm.seek 2 + 300 counts @19:34:46.18 Forearm.reconfigure LooseForearm @19:34:46.23 Hand.close @19:34:46.90 SE.seek :down @19:34:47.13
Forearm.seek :retract @19:34:48.06 Elbow.seek CC @19:34:48.14 SE.coast @19:34:49.76
Elbow.reconfigure LooseElbow @19:34:49.87 Forearm.reconfigure TightForearm @19:34:49.92 Forearm.seek CC @19:34:51.70 CC.seek :closed @19:34:55.96 Collection Clamp onSpring/offSpring for 6/32 samples, on/off torque 1.73 @19:34:55.97 Hand.open @19:34:56.70 Forearm.reconfigure LooseForearm @19:34:56.75 Forearm.seek :retract @19:34:57.74 SC.coast @19:34:57.78 CTV.dial CSV,avoiding: :intake @19:34:59.01 CSV.dial CTV @19:34:59.32 CS.seek 5ml @19:35:13.07 delay 1:20 @19:36:33.09 CS.seek 2.5ml @19:36:40.51 delay 40 seconds @19:37:20.54 CS.seek 0ml @19:37:27.95 delay 1:00 @19:38:27.97 PSV.dial :air @19:38:28.43 PS.seek 2.5ml @19:38:54.53 PSV.dial :mixloop @19:38:54.98 delay 3 seconds @19:38:57.99 PSV.dial :air @19:38:58.44 delay 2 seconds @19:39:00.46 PSV.dial :mixloop @19:39:00.91 delay 3 seconds @19:39:03.92 PSV.dial :waste1 @19:39:05.15 PS.seek :empty @19:39:31.34 CBV.dial :waste1 @19:39:31.81 delay 5 seconds @19:39:36.83 CBV.dial AIV @19:39:37.31 delay 10 seconds @19:39:47.33 CBV.dial :waste1 @19:39:47.79 delay 10 seconds @19:39:57.81 CBV.dial AIV @19:39:58.28 delay 10 seconds @19:40:08.31 CTV.dial CSV,avoiding: :intake @19:40:08.32 CBV.dial between AIV and waste1 @19:40:08.65 CSV.dial :air @19:40:09.09 CS.seek 10ml @19:40:35.60 CSV.dial CTV @19:40:36.06 CS.seek 0ml @19:41:02.63 CBV.dial :waste1 @19:41:02.95 delay 10 seconds @19:41:12.98 CTV.dial :air,avoiding: :intake @19:41:14.26 Storing lyfil puck in Collection Clamp to tube 1 @19:41:14.44 SC.reconfigure SCtight @19:41:14.49 SC.seek 1 @19:41:16.61 SC.reconfigure SCloose @19:41:16.84 Elbow.reconfigure TightElbow @19:41:16.88 Elbow.seek 1 @19:41:18.83 Forearm.reconfigure TightForearm @19:41:18.88 Forearm.seek 1 + 700 counts @19:41:19.91 SE.reconfigure SEpick @19:41:20.03 SE.seek beamBreak @19:41:22.70 SE.configure SEconfig @19:41:22.86 Elbow.reconfigure LimpElbow @19:41:22.91 Forearm.reconfigure LooseForearm @19:41:22.97 Forearm.seek 1 @19:41:23.30 Elbow.hold @19:41:23.37 Elbow.reconfigure TightElbow @19:41:23.47 SE.seek 3.09 Pucks below Top Plate @19:41:23.94 Forearm.force -20 @19:41:23.98 Forearm.seek 1 + 317 counts @19:41:24.87 Forearm.reconfigure TightForearm @19:41:24.92 Forearm.seek 1 + 700 counts @19:41:25.24 SE.seek 4.09 Pucks below Top Plate @19:41:25.89 Forearm.reconfigure LooseForearm @19:41:25.94 Forearm.seek :retract @19:41:26.84 Elbow.seek CC @19:41:28.52 Elbow.reconfigure LooseElbow @19:41:28.63 Forearm.seek CC @19:41:29.61 Hand.close @19:41:30.27 CC.seek :open @19:41:34.74 Forearm.seek :retract @19:41:35.71 Elbow.reconfigure TightElbow @19:41:35.76 Elbow.seek 1 @19:41:37.66 Elbow.reconfigure LimpElbow @19:41:37.72 Forearm.seek 1 @19:41:38.74 Elbow.hold @19:41:38.81 Elbow.reconfigure TightElbow @19:41:38.86 Hand.open @19:41:39.66 Forearm.force -20 @19:41:39.70 Forearm.seek 1 + 350 counts @19:41:40.01 Forearm.reconfigure TightForearm @19:41:40.06 Forearm.seek 1 + 700 counts @19:41:40.38 SE.seek :down @19:41:41.53
Forearm.reconfigure LooseForearm @19:41:41.58 Forearm.seek :retract @19:41:42.78 SE.coast @19:41:42.82
SC.coast @19:41:42.86 PSV.dial :air @19:41:44.00 PS.seek 0.499ml @19:41:49.84 delay 3 seconds @19:41:52.86 PSV.dial :mixloop @19:41:53.31 PRV.dial :air @19:41:53.76 delay 3 seconds @19:41:56.77 PRV.dial CBV @19:41:57.22 CBV.dial AIV @19:41:57.67 PRV.dial between CBV and air @19:41:58.01 PRV.dial :air @19:41:58.38 PS.reconfigure PSslow @19:41:58.43 PS.seek 0.599ml @19:42:00.94 PRV.dial :waste2 @19:42:01.65 PS.reconfigure PSconfig @19:42:01.70 PS.seek :empty @19:42:08.66 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:42:08.73 Hand.open @19:42:09.14 Elbow.seek FlushPuck::Garage @19:42:11.15 Elbow.reconfigure LooseElbow @19:42:11.26 Forearm.seek :garage @19:42:12.26 Hand.close @19:42:13.03 Forearm.seek :retract @19:42:14.06 Elbow.reconfigure TightElbow @19:42:14.11 Elbow.seek CC @19:42:17.60 Elbow.reconfigure LooseElbow @19:42:17.70 Forearm.reconfigure TightForearm @19:42:17.75 Forearm.seek CC @19:42:19.03 CC.seek :closed @19:42:23.28 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.67 @19:42:23.36 Hand.open @19:42:24.09 Forearm.reconfigure LooseForearm @19:42:24.14 Forearm.seek :retract @19:42:25.19 Clearing PML for modified lysate @19:42:25.21 CTV.dial :air,avoiding: :intake @19:42:25.22 PRV.dial :waste2 @19:42:25.23 PTV.dial :PRV,avoiding: :puck @19:42:25.91 delay 2 seconds @19:42:27.93 PTV.dial :PRVmixing,avoiding: :puck @19:42:28.63 delay 2 seconds @19:42:30.64 PSV.dial :PML @19:42:30.66 delay 2 seconds @19:42:32.68 PRV.dial :air @19:42:33.38 delay 2 seconds @19:42:35.46 PS.seek 1.499ml @19:42:51.53 delay 5 seconds @19:42:56.56 PS.seek 2.5ml @19:43:07.51 PSV.dial :air @19:43:07.97 PS.seek 0ml @19:43:34.10 mixing lysate with speDil @19:43:34.11 PSV.dial :PML @19:43:34.55 CBV.dial :PRV @19:43:34.57 PRV.dial :air @19:43:34.58 delay 2 seconds @19:43:36.61 PRV.dial :speDil @19:43:37.06 delay 2 seconds @19:43:39.14 PS.reconfigure PSslow1 @19:43:39.19 PS.seek 0.225ml @19:44:02.98 delay 5 seconds @19:44:08.00 PRV.dial CBV @19:44:08.73 delay 2 seconds @19:44:10.82 PS.seek 0.475ml @19:44:36.73 delay 5 seconds @19:44:41.75 PRV.dial :air @19:44:42.20 delay 2 seconds @19:44:44.22 PS.seek 0.965ml @19:45:34.17 delay 10 seconds @19:45:44.19 PRV.dial CBV @19:45:44.65 CTV.dial :air,avoiding: :intake @19:45:44.66 PSV.dial :air @19:45:45.10 PS.reconfigure PSconfig @19:45:45.15 PS.seek 2.5ml @19:46:01.60 delay 3 seconds @19:46:04.61 PSV.dial PTV @19:46:05.31 PTV.dial :PRV,avoiding: :puck @19:46:06.01 PS.seek 0ml @19:46:32.19 PSV.dial :air @19:46:32.90 PS.seek 2.5ml @19:46:58.98 delay 3 seconds @19:47:01.99 PSV.dial PTV @19:47:02.70 PTV.dial :PRV,avoiding: :puck @19:47:02.72 PS.seek 0ml @19:47:28.82 Pushing modified lysate into AIV Injection Loop @19:47:28.83 CBV.dial AIV @19:47:28.84 AIV.dial :bypass @19:47:28.85 PRV.dial CBV @19:47:28.86 PSV.dial :air @19:47:29.56 PS.seek 2.5ml @19:47:55.85 delay 3 seconds @19:47:58.87 PSV.dial :PML @19:47:59.78 PTV.dial :PRVmixing,avoiding: :puck @19:48:00.57 PS.reconfigure PSslow1 @19:48:00.62 PS.seek 1.74ml @19:49:18.36 delay 5 seconds @19:49:23.39 AIV.dial :sample @19:49:23.92 AWV.select :waste @19:49:24.10 PS.reconfigure PSslow2 @19:49:24.14 PS.seek 1.22ml @19:51:12.61 delay 15 seconds @19:51:27.63 AIV.dial :bypass @19:51:28.18 PS.reconfigure PSconfig @19:51:28.23 PS.seek 0ml @19:51:41.47 PSV.dial :air @19:51:41.90 PS.seek 2.5ml @19:52:07.97 PSV.dial :PML @19:52:08.40 PS.seek 0ml @19:52:34.47 AIV.dial :coilToMFB @19:52:34.49 AWV.select :air @19:52:34.65 Extracting Nucleic Acid from AIV @19:52:34.69
Beginning samplePassD @19:52:34.75 SPE.hold 2:46:40,55C @19:52:34.81 ASV.dial :SPEdelivery @19:52:34.89
AWV.select :air @19:52:34.90 AIV.dial :bypass @19:52:34.91 PSV.dial PSR @19:52:35.21 delay 20 seconds @19:52:35.96
Pulling 1.5ml of PSR.killMFB @19:52:35.97 PSR.select :killMFB @19:52:36.10 PS.seek 1.5ml @19:52:52.17 delay 5 seconds @19:52:55.24 primeLysateINT @19:52:55.25 AWV.select :air @19:52:55.26 AIV.dial :coilToMFB @19:52:55.27 ATV.dial AIV @19:52:56.98 AS.reconfigure ASslow1 @19:52:57.03 AS.seek 0.299ml @19:52:57.18
PSV.dial :waste1 @19:52:57.64 PS.seek 0ml @19:53:13.60 PSV.dial PSR @19:53:14.08 PSR.select :air @19:53:14.20 PS.seek 2.5ml @19:53:40.23 PRV.dial CBV @19:53:40.26
CBV.dial PRV @19:53:40.27 PSV.dial :mixloop @19:53:41.25 CTV.dial :air,avoiding: :intake @19:53:41.26 PTV.dial :PRVmixing @19:53:41.28 PS.seek 0ml @19:53:57.68 delay 10 seconds @19:54:07.35
PSV.dial PSR @19:54:07.69 ATV.dial :air2 @19:54:08.43
Pulling 1.5ml of PSR.flush @19:54:08.44 PSR.select :flush @19:54:08.58 AS.seek 0.329ml @19:54:08.65
PS.seek 1.5ml @19:54:14.86 ATV.dial :waste2 @19:54:15.40 AS.reconfigure ASnorm @19:54:15.45 AS.seek :empty @19:54:24.64
delay 5 seconds @19:54:29.66 PSV.dial :waste1 @19:54:30.12 PS.seek 0ml @19:54:32.46 Lysate primed! @19:54:32.47 AFV.select :holdingCoil @19:54:32.48 ATV.dial :air2 @19:54:33.07 AS.reconfigure ASslow1 @19:54:33.12 AS.seek 0.069ml @19:54:46.13
PSV.dial PSR @19:54:46.58 PSR.select :air @19:54:46.70 PS.seek 2.5ml @19:54:47.52 delay 3 seconds @19:54:50.53 ATV.dial :AIV @19:54:51.34 AS.seek 0.469ml @19:55:12.83 PRV.dial CBV @19:55:12.86
CBV.dial PRV @19:55:12.87 PSV.dial :mixloop @19:55:13.84 CTV.dial :air,avoiding: :intake @19:55:13.85 PTV.dial :PRVmixing @19:55:13.87 PS.seek 0ml @19:55:40.01 PSV.dial PSR @19:55:41.56 Pulling 1.5ml of PSR.flush @19:55:41.57 PSR.select :flush @19:55:41.70 PS.seek 1.5ml @19:55:57.70 delay 5 seconds @19:56:02.72 PSV.dial :waste1 @19:56:03.20 PS.seek 0ml @19:56:12.04 delay 5 seconds @19:56:17.12 Solid Phase Extraction Heater at 53.8C @19:56:17.14 ATV.dial ASV @19:56:17.51 ASV.dial :SPEdelivery @19:56:17.59 AS.seek 0.041ml @19:56:19.26
PSV.dial PSR @19:56:19.72 PSR.select :air @19:56:19.85 PS.seek 2.5ml @19:56:45.92 PRV.dial CBV @19:56:45.95
CBV.dial PRV @19:56:45.96 PSV.dial :mixloop @19:56:46.92 CTV.dial :air,avoiding: :intake @19:56:46.93 PTV.dial :PRVmixing @19:56:46.95 PS.seek 0ml @19:57:13.13 PSV.dial PSR @19:57:14.15 Pulling 1.5ml of PSR.flush @19:57:14.16 PSR.select :flush @19:57:14.29 PS.seek 1.5ml @19:57:30.30 delay 5 seconds @19:57:35.32 PSV.dial :waste1 @19:57:35.77 PS.seek 0ml @19:57:43.96 delay 10 seconds @19:57:51.73
PSV.dial PSR @19:57:52.18 PSR.select :air @19:57:52.31 PS.seek 2.5ml @19:57:53.98 rinseLysateLineINT @19:57:53.99 AFV.select :carrierSolution @19:57:54.10 delay 2 seconds @19:57:56.13 AS.reconfigure ASconfig @19:57:56.18 AS.seek 1ml @19:58:18.49 PRV.dial CBV @19:58:18.51
CBV.dial PRV @19:58:18.54 PSV.dial :mixloop @19:58:19.49 CTV.dial :air,avoiding: :intake @19:58:19.50 PTV.dial :PRVmixing @19:58:19.52 PS.reconfigure PSslow @19:58:19.57 PS.seek 1.2ml @19:58:46.45 PSV.dial :waste1 @19:58:47.66 PS.reconfigure PSconfig @19:58:47.71 PS.seek 0ml @19:58:51.58 delay 1 second @19:58:52.59 AFV.select :holdingCoil @19:58:52.70 delay 1 second @19:58:53.72 ATV.dial AIV @19:58:54.26 AIV.dial :coilToMFB @19:58:54.27 AWV.select :waste @19:58:54.38 AS.reconfigure ASslow0 @19:58:54.43 AS.seek 0ml @19:59:00.69
PSV.dial :air @19:59:01.64 PS.seek 2.5ml @19:59:27.73 PSV.dial :mixloop @19:59:28.17 PS.reconfigure PSslow @19:59:28.22 PS.seek 0ml @20:00:19.32 PSV.dial :air @20:00:19.75 PS.reconfigure PSconfig @20:00:19.80 PS.seek 2.5ml @20:00:35.33 ATV.dial :air2 @20:00:35.98 AS.reconfigure ASslow1 @20:00:36.03 AS.seek 0.05ml @20:00:45.98
PSV.dial :mixloop @20:00:46.36 ATV.dial :AIV @20:00:46.44
PS.reconfigure PSslow @20:00:46.49 PS.seek 0ml @20:00:47.27 AS.seek 0.01ml @20:00:55.61 ATV.dial :waste @20:00:57.13 AS.seek :empty @20:00:59.66 ethanol wash @20:00:59.67 ATV.dial :air2 @20:01:00.86 AS.reconfigure ASslow2 @20:01:00.92 AS.seek 0.009ml @20:01:11.16 delay 3 seconds @20:01:14.18 Pulling 0.4ml of Ethanol Wash @20:01:14.20 ATV.dial :ethanol @20:01:14.97 AS.reconfigure ASslow1 @20:01:15.02 AS.seek 0.409ml @20:01:37.74
Cleaning Collection Syringe @20:01:37.75 CSV.dial CSR @20:01:38.24 Pulling 4ml of CSR.flush @20:01:38.25 CSR.select :flush @20:01:38.39 CS.seek 4ml @20:01:49.53 delay 5 seconds @20:01:54.55 CSR.select :air @20:01:54.69 CS.seek 10ml @20:02:10.97 CSV.dial CTV @20:02:11.00
CTV.dial CSV @20:02:12.31 CBV.dial :waste1 @20:02:12.79 CS.seek 0ml @20:02:36.27 delay 3 seconds @20:02:39.30
CSV.dial CSR @20:02:39.35 ATV.dial :air1 @20:02:39.76 delay 3 seconds @20:02:39.85
Pulling 4ml of CSR.flush @20:02:39.86 CSR.select :flush @20:02:39.99 CS.seek 4ml @20:02:42.83 AS.seek 0.434ml @20:02:48.15 delay 3 seconds @20:02:51.17 ATV.dial :ethanol @20:02:51.24
delay 5 seconds @20:02:51.77 AS.seek 0.419ml @20:02:55.07 delay 3 seconds @20:02:56.27
CSR.select :air @20:02:56.39 CS.seek 10ml @20:02:58.08 ATV.dial ASV @20:02:59.34 AS.seek 0.092ml @20:03:12.69 CSV.dial CTV @20:03:12.71
CTV.dial CSV @20:03:13.17 CBV.dial :waste1 @20:03:13.19 CS.seek 0ml @20:03:39.93 Garaging FlushPuck in Collection Clamp @20:03:40.02 Elbow.reconfigure TightElbow @20:03:40.06 Elbow.seek CC @20:03:40.30 Elbow.reconfigure LooseElbow @20:03:40.41 Forearm.seek CC @20:03:41.37 Hand.close @20:03:42.05 CC.seek :open @20:03:46.38 Forearm.seek :retract @20:03:47.37 Elbow.reconfigure TightElbow @20:03:47.42 Elbow.seek :garage @20:03:50.55 Elbow.reconfigure LooseElbow @20:03:50.65 Forearm.reconfigure TightForearm @20:03:50.70 Forearm.seek :garage @20:03:51.87 Hand.open @20:03:52.62 Forearm.reconfigure LooseForearm @20:03:52.66 Forearm.seek :retract @20:03:53.68 Whole Cell Archive sampling 1500ml @20:03:53.70 Loading wcr puck from tube 2 into Collection Clamp @20:03:53.95 SC.reconfigure SCtight @20:03:54.01 SC.seek 2 @20:03:56.10 SC.reconfigure SCloose @20:03:56.34 Elbow.reconfigure TightElbow @20:03:56.39 Elbow.seek 2 @20:03:58.46 Forearm.reconfigure TightForearm @20:03:58.51 Forearm.seek 2 + 700 counts @20:03:59.59 SE.reconfigure SEpick @20:03:59.66 SE.seek beamBreak @20:04:01.10 SE.configure SEconfig @20:04:01.82 Elbow.seek 2 @20:04:02.03 Forearm.seek 2 + 300 counts @20:04:02.29 Forearm.reconfigure LooseForearm @20:04:02.34 Hand.close @20:04:02.95 SE.seek :down @20:04:03.18 Forearm.seek :retract @20:04:04.12 Elbow.seek CC @20:04:04.30 SE.coast @20:04:05.38 delay 3 seconds @20:04:05.81 Elbow.reconfigure LooseElbow @20:04:05.92 Forearm.reconfigure TightForearm @20:04:05.97 Forearm.seek CC @20:04:07.26 CC.seek :closed @20:04:08.39 ATV.dial :waste2 @20:04:09.14 AS.reconfigure ASconfig @20:04:09.22 AS.seek :empty @20:04:11.57 Collection Clamp onSpring/offSpring for 6/32 samples, on/off torque 1.70 @20:04:11.59 Hand.open @20:04:12.25 Forearm.reconfigure LooseForearm @20:04:12.30 Forearm.seek :retract @20:04:13.28 SC.coast @20:04:13.33 collectSample 1500ml w/bubble point of 55psi @20:04:13.34 Priming 7ml @20:04:13.35 Intake.select :close @20:04:13.36 Exhaust.select :close @20:04:13.38 SS.hold @20:04:13.61 Calibrating pressure sensors @20:04:13.62 CTV.dial :air @20:04:14.88 SSV.dial :spit,avoiding: :exhaust @20:04:14.91 SS.reconfigure SSfast @20:04:14.98 SS.seek 5ml @20:04:14.99 ATV.dial :air2 @20:04:15.41 AS.reconfigure ASslow1 @20:04:15.46 AS.seek 1ml @20:04:17.65 SSV.dial CBV @20:04:17.68 CBV.dial SSV @20:04:19.01 delay 3.8 seconds @20:04:22.95 SSV.dial :spit @20:04:24.18 SS.seek 0ml @20:04:26.92 SSV.dial CBV @20:04:28.18 CTV.dial :intake @20:04:28.89 SSV.dial CBV @20:04:28.92 CBV.dial SSV @20:04:28.93 Allowing 40s for 7.0ml of remaining 7.0ml @20:04:28.95 Intake.select :open @20:04:31.12 Intake @34.2psia, vacuum from 20.0 to 24.0psi @20:04:31.14 SS.reconfigure SSinhale.with maxSpeed: 150 @20:04:31.19 SS.seek 7ml @20:04:35.52 Vacuum too high -- waiting up to 40s @20:04:39.25 Intake @35.1psia, vacuum from 20.0 to 24.0psi @20:04:39.27 SS.reconfigure SSinhale.with maxSpeed: 135 @20:04:39.32 SS.seek 7ml @20:04:41.38 Stroke completed -- waiting for pressure to equalize ... @20:04:47.79 Intake.select :close @20:04:47.95 SSV.dial :exhaust @20:04:48.68 Exhaust.select :open @20:04:48.79 SS.reconfigure SSexhale @20:04:48.84 SS.seek 0ml @20:04:52.72 Exhaust.select :close @20:04:52.84 Sampling 1500.0ml @20:04:52.85 Intake.select :close @20:04:52.87 Exhaust.select :close @20:04:52.88 SS.reconfigure SSconfig @20:04:52.93 SS.hold @20:04:53.15 CTV.dial :intake @20:04:53.17 SSV.dial CBV @20:04:53.19 CBV.dial SSV @20:04:53.95 Allowing 130s for 25.0ml of remaining 1500.0ml @20:04:53.96 Intake.select :open @20:04:56.14 Intake @33.9psia, vacuum from 20.0 to 24.0psi @20:04:56.16 SS.reconfigure SSinhale.with maxSpeed: 135 @20:04:56.21 SS.seek 25ml @20:04:59.52 Vacuum too high -- waiting up to 40s @20:05:01.37 Intake @34.5psia, vacuum from 20.0 to 24.0psi @20:05:01.39 SS.reconfigure SSinhale.with maxSpeed: 122 @20:05:01.44 SS.seek 25ml @20:05:03.93 Vacuum too high -- waiting up to 40s @20:05:05.29 Intake @34.4psia, vacuum from 20.0 to 24.0psi @20:05:05.31 SS.reconfigure SSinhale.with maxSpeed: 110 @20:05:05.36 SS.seek 25ml @20:05:07.52 Vacuum too high -- waiting up to 40s @20:05:08.85 Intake @34.4psia, vacuum from 20.0 to 24.0psi @20:05:08.87 SS.reconfigure SSinhale.with maxSpeed: 99 @20:05:08.92 SS.seek 25ml @20:05:11.15 Vacuum too high -- waiting up to 40s @20:05:12.30 Intake @34.3psia, vacuum from 20.0 to 24.0psi @20:05:12.32 SS.reconfigure SSinhale.with maxSpeed: 89 @20:05:12.37 SS.seek 25ml @20:05:14.80 Vacuum too high -- waiting up to 40s @20:05:16.20 Intake @34.5psia, vacuum from 20.0 to 24.0psi @20:05:16.22 SS.reconfigure SSinhale.with maxSpeed: 80 @20:05:16.27 SS.seek 25ml @20:05:18.99 Vacuum too high -- waiting up to 40s @20:05:20.10 Intake @34.1psia, vacuum from 20.0 to 24.0psi @20:05:20.12 SS.reconfigure SSinhale.with maxSpeed: 72 @20:05:20.17 SS.seek 25ml @20:05:23.27 Vacuum too high -- waiting up to 40s @20:05:24.37 Intake @34.5psia, vacuum from 20.0 to 24.0psi @20:05:24.39 SS.reconfigure SSinhale.with maxSpeed: 65 @20:05:24.44 SS.seek 25ml @20:05:27.74 Vacuum too high -- waiting up to 40s @20:05:28.85 Intake @34.0psia, vacuum from 20.0 to 24.0psi @20:05:28.87 SS.reconfigure SSinhale.with maxSpeed: 59 @20:05:28.92 SS.seek 25ml @20:05:34.43 Vacuum too high -- waiting up to 40s @20:05:35.26 Intake @34.4psia, vacuum from 20.0 to 24.0psi @20:05:35.28 SS.reconfigure SSinhale.with maxSpeed: 53 @20:05:35.33 SS.seek 25ml @20:05:35.55 Stroke completed -- waiting for pressure to equalize ... @20:05:38.15 Intake.select :close @20:05:38.31 SSV.dial :exhaust @20:05:39.05 Exhaust.select :open @20:05:39.17 SS.reconfigure SSexhale @20:05:39.22 SS.seek 0ml @20:05:48.16 Exhaust.select :close @20:05:48.28 SSV.dial CBV @20:05:48.31 CBV.dial SSV @20:05:49.07 Allowing 130s for 25.0ml of remaining 1475.0ml @20:05:49.08 Intake.select :open @20:05:51.26 Intake @33.6psia, vacuum from 20.0 to 24.0psi @20:05:51.28 SS.reconfigure SSinhale.with maxSpeed: 53 @20:05:51.33 SS.seek 25ml @20:06:11.21 Vacuum too high -- waiting up to 40s @20:06:12.29 Intake @34.1psia, vacuum from 20.0 to 24.0psi @20:06:12.31 SS.reconfigure SSinhale.with maxSpeed: 48 @20:06:12.36 SS.seek 25ml @20:06:27.81 Stroke completed -- waiting for pressure to equalize ... @20:06:30.32 Intake.select :close @20:06:30.49 SSV.dial :exhaust @20:06:31.22 Exhaust.select :open @20:06:31.33 SS.reconfigure SSexhale @20:06:31.38 SS.seek 0ml @20:06:40.06 Exhaust.select :close @20:06:40.18 SSV.dial CBV @20:06:40.21 CBV.dial SSV @20:06:40.97 Allowing 130s for 25.0ml of remaining 1450.0ml @20:06:40.98 Intake.select :open @20:06:43.16 Intake @33.9psia, vacuum from 20.0 to 24.0psi @20:06:43.18 SS.reconfigure SSinhale.with maxSpeed: 48 @20:06:43.23 SS.seek 25ml @20:07:20.58 Stroke completed -- waiting for pressure to equalize ... @20:07:23.19 Intake.select :close @20:07:23.35 SSV.dial :exhaust @20:07:24.07 Exhaust.select :open @20:07:24.18 SS.reconfigure SSexhale @20:07:24.23 SS.seek 0ml @20:07:33.02 Exhaust.select :close @20:07:33.14 SSV.dial CBV @20:07:33.17 CBV.dial SSV @20:07:33.93 Allowing 130s for 25.0ml of remaining 1425.0ml @20:07:33.94 Intake.select :open @20:07:36.12 Intake @34.0psia, vacuum from 20.0 to 24.0psi @20:07:36.14 SS.reconfigure SSinhale.with maxSpeed: 48 @20:07:36.19 SS.seek 25ml @20:07:37.06 delay 5 seconds @20:07:42.08 ATV.dial ASV @20:07:42.98 ASV.dial :SPEdelivery @20:07:43.01 AS.seek 0.02ml @20:08:13.56 Stroke completed -- waiting for pressure to equalize ... @20:08:16.17 Intake.select :close @20:08:16.32 SSV.dial :exhaust @20:08:17.05 Exhaust.select :open @20:08:17.17 SS.reconfigure SSexhale @20:08:17.22 SS.seek 0ml @20:08:26.20 Exhaust.select :close @20:08:26.32 SSV.dial CBV @20:08:26.35 CBV.dial SSV @20:08:27.11 Allowing 130s for 25.0ml of remaining 1400.0ml @20:08:27.12 Intake.select :open @20:08:29.30 Intake @33.7psia, vacuum from 20.0 to 24.0psi @20:08:29.32 SS.reconfigure SSinhale.with maxSpeed: 48 @20:08:29.37 SS.seek 25ml @20:09:06.52 Stroke completed -- waiting for pressure to equalize ... @20:09:09.11 Intake.select :close @20:09:09.26 SSV.dial :exhaust @20:09:09.99 Exhaust.select :open @20:09:10.11 SS.reconfigure SSexhale @20:09:10.16 SS.seek 0ml @20:09:19.05 Exhaust.select :close @20:09:19.17 SSV.dial CBV @20:09:19.20 CBV.dial SSV @20:09:19.95 Allowing 130s for 25.0ml of remaining 1375.0ml @20:09:19.96 Intake.select :open @20:09:22.14 Intake @34.3psia, vacuum from 20.0 to 24.0psi @20:09:22.16 SS.reconfigure SSinhale.with maxSpeed: 48 @20:09:22.21 SS.seek 25ml @20:09:59.19 Stroke completed -- waiting for pressure to equalize ... @20:10:02.31 Intake.select :close @20:10:02.47 SSV.dial :exhaust @20:10:03.21 Exhaust.select :open @20:10:03.32 SS.reconfigure SSexhale @20:10:03.38 SS.seek 0ml @20:10:12.28 Exhaust.select :close @20:10:12.40 SSV.dial CBV @20:10:12.43 CBV.dial SSV @20:10:13.21 Allowing 130s for 25.0ml of remaining 1350.0ml @20:10:13.22 Intake.select :open @20:10:15.40 Intake @34.3psia, vacuum from 20.0 to 24.0psi @20:10:15.43 SS.reconfigure SSinhale.with maxSpeed: 48 @20:10:15.48 SS.seek 25ml @20:10:52.66 Stroke completed -- waiting for pressure to equalize ... @20:10:55.89 Intake.select :close @20:10:56.05 SSV.dial :exhaust @20:10:56.81 Exhaust.select :open @20:10:56.92 SS.reconfigure SSexhale @20:10:56.97 SS.seek 0ml @20:11:00.37 delay 5 seconds @20:11:05.39 ATV.dial :air2 @20:11:06.02 Exhaust.select :close @20:11:06.14 SSV.dial CBV @20:11:06.17 CBV.dial SSV @20:11:06.39 AS.seek 0.52ml @20:11:06.93 Allowing 130s for 25.0ml of remaining 1325.0ml @20:11:06.94 Intake.select :open @20:11:09.13 Intake @34.2psia, vacuum from 20.0 to 24.0psi @20:11:09.15 SS.reconfigure SSinhale.with maxSpeed: 48 @20:11:09.20 SS.seek 25ml @20:11:46.28 Stroke completed -- waiting for pressure to equalize ... @20:11:49.49 Intake.select :close @20:11:49.64 SSV.dial :exhaust @20:11:50.38 Exhaust.select :open @20:11:50.50 SS.reconfigure SSexhale @20:11:50.55 SS.seek 0ml @20:11:59.52 Exhaust.select :close @20:11:59.64 SSV.dial CBV @20:11:59.67 CBV.dial SSV @20:12:00.42 Allowing 130s for 25.0ml of remaining 1300.0ml @20:12:00.43 Intake.select :open @20:12:02.61 Intake @33.9psia, vacuum from 20.0 to 24.0psi @20:12:02.63 SS.reconfigure SSinhale.with maxSpeed: 48 @20:12:02.68 SS.seek 25ml @20:12:39.67 Stroke completed -- waiting for pressure to equalize ... @20:12:42.90 Intake.select :close @20:12:43.05 SSV.dial :exhaust @20:12:43.79 Exhaust.select :open @20:12:43.90 SS.reconfigure SSexhale @20:12:43.95 SS.seek 0ml @20:12:47.24 delay 5 seconds @20:12:52.26 ATV.dial ASV @20:12:52.95 Exhaust.select :close @20:12:53.07 SSV.dial CBV @20:12:53.10 CBV.dial SSV @20:12:53.30 ASV.dial :wasteR @20:12:53.68 AS.seek 0.3ml @20:12:53.86 Allowing 130s for 25.0ml of remaining 1275.0ml @20:12:53.87 Intake.select :open @20:12:56.04 Intake @33.8psia, vacuum from 20.0 to 24.0psi @20:12:56.06 SS.reconfigure SSinhale.with maxSpeed: 48 @20:12:56.65 SS.seek 25ml @20:13:33.84 Stroke completed -- waiting for pressure to equalize ... @20:13:37.08 Intake.select :close @20:13:37.24 SSV.dial :exhaust @20:13:37.96 Exhaust.select :open @20:13:38.07 SS.reconfigure SSexhale @20:13:38.12 SS.seek 0ml @20:13:38.21 delay 20 seconds @20:13:47.27 Exhaust.select :close @20:13:47.39 SSV.dial CBV @20:13:47.42 CBV.dial SSV @20:13:48.18 Allowing 130s for 25.0ml of remaining 1250.0ml @20:13:48.19 Intake.select :open @20:13:50.37 Intake @33.8psia, vacuum from 20.0 to 24.0psi @20:13:50.39 SS.reconfigure SSinhale.with maxSpeed: 48 @20:13:50.44 SS.seek 25ml @20:13:58.24 AS.seek 0.07ml @20:14:27.64 Stroke completed -- waiting for pressure to equalize ... @20:14:31.51 Intake.select :close @20:14:31.66 SSV.dial :exhaust @20:14:32.39 Exhaust.select :open @20:14:32.51 SS.reconfigure SSexhale @20:14:32.56 SS.seek 0ml @20:14:41.52 Exhaust.select :close @20:14:41.64 SSV.dial CBV @20:14:41.67 CBV.dial SSV @20:14:42.43 Allowing 130s for 25.0ml of remaining 1225.0ml @20:14:42.44 Intake.select :open @20:14:44.62 Intake @34.2psia, vacuum from 20.0 to 24.0psi @20:14:44.64 SS.reconfigure SSinhale.with maxSpeed: 48 @20:14:44.69 SS.seek 25ml @20:14:44.77 delay 20 seconds @20:15:04.79 ATV.dial :waste2 @20:15:05.50 AS.reconfigure ASconfig @20:15:05.55 AS.seek :empty @20:15:09.94 Elution @20:15:09.95 ATV.dial :air1 @20:15:10.99 AS.reconfigure ASslow1 @20:15:11.04 AS.seek 0.049ml @20:15:17.97 Vacuum too high -- waiting up to 40s @20:15:19.13 Intake @33.8psia, vacuum from 20.0 to 24.0psi @20:15:19.15 SS.reconfigure SSinhale.with maxSpeed: 43 @20:15:19.20 SS.seek 25ml @20:15:21.42 delay 3 seconds @20:15:23.71 Stroke completed -- waiting for pressure to equalize ... @20:15:24.43 ATV.dial :ElutionWater @20:15:25.09 AS.seek 0.109ml @20:15:26.30 Intake.select :close @20:15:26.46 SSV.dial :exhaust @20:15:27.21 Exhaust.select :open @20:15:27.32 SS.reconfigure SSexhale @20:15:27.37 SS.seek 0ml @20:15:36.09 Exhaust.select :close @20:15:36.21 SSV.dial CBV @20:15:36.24 CBV.dial SSV @20:15:37.01 Allowing 130s for 25.0ml of remaining 1200.0ml @20:15:37.02 Intake.select :open @20:15:37.42 delay 3 seconds @20:15:39.20 Intake @33.8psia, vacuum from 20.0 to 24.0psi @20:15:39.22 SS.reconfigure SSinhale.with maxSpeed: 43 @20:15:39.26 SS.seek 25ml @20:15:40.99 ATV.dial ASV,via: :air2 @20:15:42.90 ASV.dial :SPEelution @20:15:43.45 AFV.select :carrierSolution @20:15:43.62 AS.reconfigure ASnorm @20:15:43.67 AS.seek 0.759ml @20:16:16.73 delay 3 seconds @20:16:19.75 AFV.select :holdingCoil @20:16:19.91 AS.reconfigure ASslow1 @20:16:19.97 AS.seek 0.474ml @20:16:20.88 Stroke completed -- waiting for pressure to equalize ... @20:16:24.12 Intake.select :close @20:16:24.29 SSV.dial :exhaust @20:16:25.03 Exhaust.select :open @20:16:25.14 SS.reconfigure SSexhale @20:16:25.19 SS.seek 0ml @20:16:33.91 Exhaust.select :close @20:16:34.03 SSV.dial CBV @20:16:34.06 CBV.dial SSV @20:16:34.82 Allowing 130s for 25.0ml of remaining 1175.0ml @20:16:34.83 Intake.select :open @20:16:37.02 Intake @34.0psia, vacuum from 20.0 to 24.0psi @20:16:37.04 SS.reconfigure SSinhale.with maxSpeed: 43 @20:16:37.08 SS.seek 25ml @20:17:17.56 delay 5 seconds @20:17:18.56 Stroke completed -- waiting for pressure to equalize ... @20:17:21.79 Intake.select :close @20:17:21.95 SSV.dial :exhaust @20:17:22.64 AS.reconfigure ASslow2 @20:17:22.72 AS.seek 0.439ml @20:17:22.75 Exhaust.select :open @20:17:22.91 SS.reconfigure SSexhale @20:17:22.96 SS.seek 0ml @20:17:31.97 Exhaust.select :close @20:17:32.09 SSV.dial CBV @20:17:32.12 CBV.dial SSV @20:17:32.90 Allowing 130s for 25.0ml of remaining 1150.0ml @20:17:32.91 Intake.select :open @20:17:35.10 Intake @34.0psia, vacuum from 20.0 to 24.0psi @20:17:35.12 SS.reconfigure SSinhale.with maxSpeed: 43 @20:17:35.17 SS.seek 25ml @20:17:58.12 delay 1:00 @20:18:16.52 Stroke completed -- waiting for pressure to equalize ... @20:18:19.75 Intake.select :close @20:18:19.90 SSV.dial :exhaust @20:18:20.65 Exhaust.select :open @20:18:20.76 SS.reconfigure SSexhale @20:18:20.81 SS.seek 0ml @20:18:29.58 Exhaust.select :close @20:18:29.70 SSV.dial CBV @20:18:29.73 CBV.dial SSV @20:18:30.51 Allowing 130s for 25.0ml of remaining 1125.0ml @20:18:30.52 Intake.select :open @20:18:32.70 Intake @34.3psia, vacuum from 20.0 to 24.0psi @20:18:32.72 SS.reconfigure SSinhale.with maxSpeed: 43 @20:18:32.77 SS.seek 25ml @20:18:58.15 pushing eluate to holding coil @20:18:58.22 AS.seek 0.279ml @20:19:14.33 Stroke completed -- waiting for pressure to equalize ... @20:19:18.09 Intake.select :close @20:19:18.26 SSV.dial :exhaust @20:19:19.01 Exhaust.select :open @20:19:19.12 SS.reconfigure SSexhale @20:19:19.17 SS.seek 0ml @20:19:27.78 Exhaust.select :close @20:19:27.90 SSV.dial CBV @20:19:27.93 CBV.dial SSV @20:19:28.71 Allowing 130s for 25.0ml of remaining 1100.0ml @20:19:28.72 Intake.select :open @20:19:30.91 Intake @34.3psia, vacuum from 20.0 to 24.0psi @20:19:30.93 SS.reconfigure SSinhale.with maxSpeed: 43 @20:19:30.98 SS.seek 25ml @20:20:12.58 Stroke completed -- waiting for pressure to equalize ... @20:20:15.80 Intake.select :close @20:20:15.97 SSV.dial :exhaust @20:20:16.70 Exhaust.select :open @20:20:16.82 SS.reconfigure SSexhale @20:20:16.87 SS.seek 0ml @20:20:25.69 Exhaust.select :close @20:20:25.81 SSV.dial CBV @20:20:25.84 CBV.dial SSV @20:20:26.62 Allowing 130s for 25.0ml of remaining 1075.0ml @20:20:26.63 Intake.select :open @20:20:28.81 Intake @34.5psia, vacuum from 20.0 to 24.0psi @20:20:28.83 SS.reconfigure SSinhale.with maxSpeed: 43 @20:20:28.88 SS.seek 25ml @20:21:10.44 Stroke completed -- waiting for pressure to equalize ... @20:21:14.28 Intake.select :close @20:21:14.43 SSV.dial :exhaust @20:21:15.17 Exhaust.select :open @20:21:15.29 SS.reconfigure SSexhale @20:21:15.34 SS.seek 0ml @20:21:24.25 Exhaust.select :close @20:21:24.37 SSV.dial CBV @20:21:24.40 CBV.dial SSV @20:21:25.17 Allowing 130s for 25.0ml of remaining 1050.0ml @20:21:25.18 Intake.select :open @20:21:27.37 Intake @34.2psia, vacuum from 20.0 to 24.0psi @20:21:27.38 SS.reconfigure SSinhale.with maxSpeed: 43 @20:21:27.43 SS.seek 25ml @20:21:39.31 delay 30 seconds @20:22:08.64 Stroke completed -- waiting for pressure to equalize ... @20:22:09.33 ATV.dial :waste,via: :air3 @20:22:10.90 AS.reconfigure ASconfig @20:22:10.95 AS.seek :empty @20:22:12.50 Intake.select :close @20:22:12.66 SSV.dial :exhaust @20:22:13.39 Exhaust.select :open @20:22:13.51 SS.reconfigure SSexhale @20:22:13.56 SS.seek 0ml @20:22:22.30 Exhaust.select :close @20:22:22.42 SSV.dial CBV @20:22:22.45 CBV.dial SSV @20:22:23.74 Allowing 130s for 25.0ml of remaining 1025.0ml @20:22:23.75 Intake.select :open @20:22:25.94 Intake @34.0psia, vacuum from 20.0 to 24.0psi @20:22:25.96 SS.reconfigure SSinhale.with maxSpeed: 43 @20:22:26.01 SS.seek 25ml @20:22:27.37 Parking Bleach in LysateSource line and SPEcolumn @20:22:27.38 ATV.dial :air3 @20:22:28.16 AS.reconfigure ASslow1 @20:22:28.21 AS.seek 0.029ml @20:22:34.56 ATV.dial :ElutionWater @20:22:35.15 AS.seek 0.059ml @20:22:41.46 ATV.dial :air3 @20:22:42.05 AS.seek 0.069ml @20:22:44.34 delay 2 seconds @20:22:46.35 ATV.dial :bleach @20:22:46.83 AS.seek 0.769ml @20:23:07.58 Stroke completed -- waiting for pressure to equalize ... @20:23:12.08 Intake.select :close @20:23:12.23 SSV.dial :exhaust @20:23:12.96 Exhaust.select :open @20:23:13.08 SS.reconfigure SSexhale @20:23:13.13 SS.seek 0ml @20:23:21.86 Exhaust.select :close @20:23:21.98 SSV.dial CBV @20:23:22.01 CBV.dial SSV @20:23:22.78 Allowing 130s for 25.0ml of remaining 1000.0ml @20:23:22.79 Intake.select :open @20:23:24.97 Intake @34.7psia, vacuum from 20.0 to 24.0psi @20:23:24.99 SS.reconfigure SSinhale.with maxSpeed: 43 @20:23:25.04 SS.seek 25ml @20:23:59.80 Vacuum too high -- waiting up to 40s @20:24:01.14 Intake @34.1psia, vacuum from 20.0 to 24.0psi @20:24:01.16 SS.reconfigure SSinhale.with maxSpeed: 39 @20:24:01.21 SS.seek 25ml @20:24:08.98 Stroke completed -- waiting for pressure to equalize ... @20:24:12.22 Intake.select :close @20:24:12.38 SSV.dial :exhaust @20:24:13.10 Exhaust.select :open @20:24:13.21 SS.reconfigure SSexhale @20:24:13.26 SS.seek 0ml @20:24:21.95 Exhaust.select :close @20:24:22.07 SSV.dial CBV @20:24:22.10 CBV.dial SSV @20:24:22.85 Allowing 130s for 25.0ml of remaining 975.0ml @20:24:22.86 Intake.select :open @20:24:25.04 Intake @34.2psia, vacuum from 20.0 to 24.0psi @20:24:25.06 SS.reconfigure SSinhale.with maxSpeed: 39 @20:24:25.11 SS.seek 25ml @20:24:53.01 Session Begun @20:24:57.30 -> showlog 100 @20:25:07.84 delay 3 seconds @20:25:10.86 ATV.dial :air3 @20:25:10.95 Stroke completed -- waiting for pressure to equalize ... @20:25:11.43 delay 3 seconds @20:25:14.85 AS.seek 0.794ml @20:25:14.98 -> exit @20:25:15.01 Intake.select :close @20:25:15.05 Session Ended @20:25:15.23 SSV.dial :exhaust @20:25:15.98 Exhaust.select :open @20:25:16.09 SS.reconfigure SSexhale @20:25:16.15 SS.seek 0ml @20:25:20.17 delay 3 seconds @20:25:23.19 ATV.dial :bleach @20:25:23.80 AS.seek 0.779ml @20:25:24.92 Exhaust.select :close @20:25:25.04 SSV.dial CBV @20:25:25.07 CBV.dial SSV @20:25:25.84 Allowing 130s for 25.0ml of remaining 950.0ml @20:25:25.85 Intake.select :open @20:25:27.11 delay 3 seconds @20:25:28.04 Intake @34.7psia, vacuum from 20.0 to 24.0psi @20:25:28.06 SS.reconfigure SSinhale.with maxSpeed: 39 @20:25:28.11 SS.seek 25ml @20:25:30.12 ATV.dial :AIV @20:25:31.39 AS.reconfigure ASconfig @20:25:31.44 AS.seek 0.015ml @20:26:13.80 Stroke completed -- waiting for pressure to equalize ... @20:26:15.73 ATV.dial :waste @20:26:17.23 AS.seek :empty @20:26:17.70 Intake.select :close @20:26:17.86 SSV.dial :exhaust @20:26:18.44 ATV.dial :air3 @20:26:18.60 Exhaust.select :open @20:26:18.71 SS.reconfigure SSexhale @20:26:18.76 SS.seek 0ml @20:26:19.10 AS.reconfigure ASslow1 @20:26:19.15 AS.seek 0.009ml @20:26:21.45 delay 2 seconds @20:26:23.47 ATV.dial :bleach @20:26:23.91 AS.seek 0.579ml @20:26:27.48 Exhaust.select :close @20:26:27.60 SSV.dial CBV @20:26:27.63 CBV.dial SSV @20:26:28.40 Allowing 130s for 25.0ml of remaining 925.0ml @20:26:28.41 Intake.select :open @20:26:30.59 Intake @34.3psia, vacuum from 20.0 to 24.0psi @20:26:30.61 SS.reconfigure SSinhale.with maxSpeed: 39 @20:26:30.66 SS.seek 25ml @20:27:16.25 Stroke completed -- waiting for pressure to equalize ... @20:27:20.71 Intake.select :close @20:27:20.88 SSV.dial :exhaust @20:27:21.63 Exhaust.select :open @20:27:21.74 SS.reconfigure SSexhale @20:27:21.79 SS.seek 0ml @20:27:30.52 Exhaust.select :close @20:27:30.64 SSV.dial CBV @20:27:30.67 CBV.dial SSV @20:27:31.45 Allowing 130s for 25.0ml of remaining 900.0ml @20:27:31.46 Intake.select :open @20:27:33.63 Intake @34.8psia, vacuum from 20.0 to 24.0psi @20:27:33.65 SS.reconfigure SSinhale.with maxSpeed: 39 @20:27:33.70 SS.seek 25ml @20:28:18.78 delay 3 seconds @20:28:19.45 Stroke completed -- waiting for pressure to equalize ... @20:28:21.87 ATV.dial :air3 @20:28:22.40 delay 3 seconds @20:28:23.92 Intake.select :close @20:28:24.09 SSV.dial :exhaust @20:28:24.84 Exhaust.select :open @20:28:24.95 SS.reconfigure SSexhale @20:28:25.00 SS.seek 0ml @20:28:25.53 AS.seek 0.604ml @20:28:30.83 delay 3 seconds @20:28:33.66 Exhaust.select :close @20:28:33.78 SSV.dial CBV @20:28:33.81 CBV.dial SSV @20:28:33.87 ATV.dial :bleach @20:28:34.47 AS.seek 0.589ml @20:28:34.59 Allowing 130s for 25.0ml of remaining 875.0ml @20:28:34.60 Intake.select :open @20:28:36.79 Intake @34.7psia, vacuum from 20.0 to 24.0psi @20:28:36.81 SS.reconfigure SSinhale.with maxSpeed: 39 @20:28:36.86 SS.seek 25ml @20:28:37.75 delay 3 seconds @20:28:40.76 ATV.dial :ASV @20:28:41.84 ASV.dial :wasteR @20:28:42.40 AS.seek 0ml @20:29:18.99 Vacuum too high -- waiting up to 40s @20:29:20.35 Intake @34.5psia, vacuum from 20.0 to 24.0psi @20:29:20.37 SS.reconfigure SSinhale.with maxSpeed: 35 @20:29:20.42 SS.seek 25ml @20:29:24.62 Stroke completed -- waiting for pressure to equalize ... @20:29:28.45 Intake.select :close @20:29:28.61 SSV.dial :exhaust @20:29:29.33 Exhaust.select :open @20:29:29.45 SS.reconfigure SSexhale @20:29:29.50 SS.seek 0ml @20:29:38.55 Exhaust.select :close @20:29:38.67 SSV.dial CBV @20:29:38.70 CBV.dial SSV @20:29:39.46 Allowing 130s for 25.0ml of remaining 850.0ml @20:29:39.47 Intake.select :open @20:29:41.65 Intake @34.7psia, vacuum from 20.0 to 24.0psi @20:29:41.67 SS.reconfigure SSinhale.with maxSpeed: 35 @20:29:41.72 SS.seek 25ml @20:30:32.20 Stroke completed -- waiting for pressure to equalize ... @20:30:36.69 Intake.select :close @20:30:36.85 SSV.dial :exhaust @20:30:37.61 Exhaust.select :open @20:30:37.72 SS.reconfigure SSexhale @20:30:37.77 SS.seek 0ml @20:30:41.17 delay 1:00 @20:30:46.69 Exhaust.select :close @20:30:46.81 SSV.dial CBV @20:30:46.84 CBV.dial SSV @20:30:48.17 Allowing 130s for 25.0ml of remaining 825.0ml @20:30:48.19 Intake.select :open @20:30:50.36 Intake @35.2psia, vacuum from 20.0 to 24.0psi @20:30:50.39 SS.reconfigure SSinhale.with maxSpeed: 35 @20:30:50.43 SS.seek 25ml @20:31:40.83 Stroke completed -- waiting for pressure to equalize ... @20:31:41.18 AFV.select :carrierSolution @20:31:41.29 delay 2 seconds @20:31:43.37 AS.reconfigure ASconfig @20:31:43.45 AS.seek 1ml @20:31:44.78 Intake.select :close @20:31:44.94 SSV.dial :exhaust @20:31:45.68 Exhaust.select :open @20:31:45.79 SS.reconfigure SSexhale @20:31:45.86 SS.seek 0ml @20:31:54.56 Exhaust.select :close @20:31:54.68 SSV.dial CBV @20:31:54.71 CBV.dial SSV @20:31:55.48 Allowing 130s for 25.0ml of remaining 800.0ml @20:31:55.49 Intake.select :open @20:31:57.67 Intake @34.4psia, vacuum from 20.0 to 24.0psi @20:31:57.69 SS.reconfigure SSinhale.with maxSpeed: 35 @20:31:57.74 SS.seek 25ml @20:32:41.20 delay 1 second @20:32:42.21 AFV.select :holdingCoil @20:32:42.32 delay 1 second @20:32:43.33 ATV.dial ASV @20:32:43.35 ASV.dial :wasteR @20:32:43.37 AS.reconfigure ASslow1 @20:32:43.41 AS.seek 0ml @20:32:48.51 Stroke completed -- waiting for pressure to equalize ... @20:32:53.01 Intake.select :close @20:32:53.17 SSV.dial :exhaust @20:32:53.93 Exhaust.select :open @20:32:54.05 SS.reconfigure SSexhale @20:32:54.10 SS.seek 0ml @20:33:02.76 Exhaust.select :close @20:33:02.88 SSV.dial CBV @20:33:02.91 CBV.dial SSV @20:33:03.68 Allowing 130s for 25.0ml of remaining 775.0ml @20:33:03.69 Intake.select :open @20:33:05.87 Intake @34.7psia, vacuum from 20.0 to 24.0psi @20:33:05.89 SS.reconfigure SSinhale.with maxSpeed: 35 @20:33:05.94 SS.seek 25ml @20:33:56.76 Stroke completed -- waiting for pressure to equalize ... @20:34:01.86 Intake.select :close @20:34:02.02 SSV.dial :exhaust @20:34:02.76 Exhaust.select :open @20:34:02.87 SS.reconfigure SSexhale @20:34:02.92 SS.seek 0ml @20:34:11.56 Exhaust.select :close @20:34:11.68 SSV.dial CBV @20:34:11.71 CBV.dial SSV @20:34:12.48 Allowing 130s for 25.0ml of remaining 750.0ml @20:34:12.49 Intake.select :open @20:34:14.67 Intake @34.8psia, vacuum from 20.0 to 24.0psi @20:34:14.69 SS.reconfigure SSinhale.with maxSpeed: 35 @20:34:14.74 SS.seek 25ml @20:34:57.21 Vacuum too high -- waiting up to 40s @20:34:58.86 Intake @34.6psia, vacuum from 20.0 to 24.0psi @20:34:58.89 SS.reconfigure SSinhale.with maxSpeed: 32 @20:34:58.94 SS.seek 25ml @20:35:08.20 Stroke completed -- waiting for pressure to equalize ... @20:35:12.72 Intake.select :close @20:35:12.88 SSV.dial :exhaust @20:35:13.63 Exhaust.select :open @20:35:13.75 SS.reconfigure SSexhale @20:35:13.80 SS.seek 0ml @20:35:22.70 Exhaust.select :close @20:35:22.82 SSV.dial CBV @20:35:22.85 CBV.dial SSV @20:35:23.62 Allowing 130s for 25.0ml of remaining 725.0ml @20:35:23.63 Intake.select :open @20:35:25.81 Intake @34.7psia, vacuum from 20.0 to 24.0psi @20:35:25.83 SS.reconfigure SSinhale.with maxSpeed: 32 @20:35:25.88 SS.seek 25ml @20:36:04.77 delay 20 seconds @20:36:20.87 Stroke completed -- waiting for pressure to equalize ... @20:36:24.78 ATV.dial :air3 @20:36:26.03 Intake.select :close @20:36:26.20 SSV.dial :exhaust @20:36:26.33 AS.seek 0.59ml @20:36:27.01 Exhaust.select :open @20:36:27.12 SS.reconfigure SSexhale @20:36:27.17 SS.seek 0ml @20:36:35.84 Exhaust.select :close @20:36:35.96 SSV.dial CBV @20:36:35.99 CBV.dial SSV @20:36:36.76 Allowing 130s for 25.0ml of remaining 700.0ml @20:36:36.77 Intake.select :open @20:36:38.95 Intake @34.8psia, vacuum from 20.0 to 24.0psi @20:36:38.98 SS.reconfigure SSinhale.with maxSpeed: 32 @20:36:39.02 SS.seek 25ml @20:37:34.39 Stroke completed -- waiting for pressure to equalize ... @20:37:40.13 Intake.select :close @20:37:40.28 SSV.dial :exhaust @20:37:41.03 Exhaust.select :open @20:37:41.15 SS.reconfigure SSexhale @20:37:41.20 SS.seek 0ml @20:37:50.25 Exhaust.select :close @20:37:50.37 SSV.dial CBV @20:37:50.40 CBV.dial SSV @20:37:51.74 Allowing 130s for 25.0ml of remaining 675.0ml @20:37:51.75 Intake.select :open @20:37:53.93 Intake @34.8psia, vacuum from 20.0 to 24.0psi @20:37:53.96 SS.reconfigure SSinhale.with maxSpeed: 32 @20:37:54.00 SS.seek 25ml @20:38:25.27 delay 3 seconds @20:38:28.29 ATV.dial :ASV @20:38:29.59 ASV.dial :wasteR @20:38:29.67 AS.seek 0.09ml @20:38:48.80 Stroke completed -- waiting for pressure to equalize ... @20:38:54.58 Intake.select :close @20:38:54.75 SSV.dial :exhaust @20:38:55.49 Exhaust.select :open @20:38:55.60 SS.reconfigure SSexhale @20:38:55.65 SS.seek 0ml @20:39:04.61 Exhaust.select :close @20:39:04.73 SSV.dial CBV @20:39:04.76 CBV.dial SSV @20:39:05.52 Allowing 130s for 25.0ml of remaining 650.0ml @20:39:05.53 Intake.select :open @20:39:07.71 Intake @34.5psia, vacuum from 20.0 to 24.0psi @20:39:07.73 SS.reconfigure SSinhale.with maxSpeed: 32 @20:39:07.78 SS.seek 25ml @20:39:52.74 Vacuum too high -- waiting up to 40s @20:39:54.84 Intake @35.2psia, vacuum from 20.0 to 24.0psi @20:39:54.86 SS.reconfigure SSinhale.with maxSpeed: 29 @20:39:54.91 SS.seek 25ml @20:40:06.03 Stroke completed -- waiting for pressure to equalize ... @20:40:10.54 Intake.select :close @20:40:10.56 delay 30 seconds @20:40:10.73 SSV.dial :exhaust @20:40:11.47 Exhaust.select :open @20:40:11.59 SS.reconfigure SSexhale @20:40:11.64 SS.seek 0ml @20:40:20.34 Exhaust.select :close @20:40:20.46 SSV.dial CBV @20:40:20.49 CBV.dial SSV @20:40:21.24 Allowing 130s for 25.0ml of remaining 625.0ml @20:40:21.25 Intake.select :open @20:40:23.44 Intake @34.4psia, vacuum from 20.0 to 24.0psi @20:40:23.46 SS.reconfigure SSinhale.with maxSpeed: 29 @20:40:23.51 SS.seek 25ml @20:40:40.59 ATV.dial :extSrc @20:40:41.31 delay 3 seconds @20:40:44.33 ATV.dial :ElutionWater @20:40:45.38 AS.seek 0.12ml @20:40:51.72 ATV.dial :air3 @20:40:52.32 AS.seek 0.13ml @20:40:54.59 delay 2 seconds @20:40:56.60 ATV.dial :bleach @20:40:57.70 AS.seek 0.23ml @20:41:18.11 delay 3 seconds @20:41:21.13 ATV.dial :air3 @20:41:21.64 delay 3 seconds @20:41:24.45 Stroke completed -- waiting for pressure to equalize ... @20:41:24.72 AS.seek 0.255ml @20:41:30.02 delay 3 seconds @20:41:30.22 Intake.select :close @20:41:30.39 SSV.dial :exhaust @20:41:31.13 Exhaust.select :open @20:41:31.24 SS.reconfigure SSexhale @20:41:31.29 SS.seek 0ml @20:41:33.04 ATV.dial :bleach @20:41:33.65 AS.seek 0.24ml @20:41:36.94 delay 3 seconds @20:41:39.96 ATV.dial :ASV @20:41:40.06 Exhaust.select :close @20:41:40.18 SSV.dial CBV @20:41:40.21 CBV.dial SSV @20:41:40.99 Allowing 130s for 25.0ml of remaining 600.0ml @20:41:41.01 Intake.select :open @20:41:41.07 ASV.dial :SPEdelivery @20:41:41.44 AFV.select :carrierSolution @20:41:41.56 delay 2 seconds @20:41:43.19 Intake @35.0psia, vacuum from 20.0 to 24.0psi @20:41:43.21 SS.reconfigure SSinhale.with maxSpeed: 29 @20:41:43.26 SS.seek 25ml @20:41:43.58 AS.reconfigure ASconfig @20:41:43.63 AS.seek 0.5ml @20:41:58.88 delay 1 second @20:41:59.89 AFV.select :holdingCoil @20:42:00.05 AS.reconfigure ASslow1 @20:42:00.10 AS.seek 0.17ml @20:42:44.14 Stroke completed -- waiting for pressure to equalize ... @20:42:50.50 Intake.select :close @20:42:50.65 SSV.dial :exhaust @20:42:51.40 Exhaust.select :open @20:42:51.51 SS.reconfigure SSexhale @20:42:51.56 SS.seek 0ml @20:43:00.92 Exhaust.select :close @20:43:01.04 SSV.dial CBV @20:43:01.07 CBV.dial SSV @20:43:01.84 Allowing 130s for 25.0ml of remaining 575.0ml @20:43:01.85 Intake.select :open @20:43:04.03 Intake @35.3psia, vacuum from 20.0 to 24.0psi @20:43:04.05 SS.reconfigure SSinhale.with maxSpeed: 29 @20:43:04.10 SS.seek 25ml @20:43:06.70 SPE.hold 2:46:40,95C @20:43:06.74 delay 3:00 @20:43:42.83 Vacuum too high -- waiting up to 40s @20:43:44.99 Intake @34.9psia, vacuum from 20.0 to 24.0psi @20:43:45.01 SS.reconfigure SSinhale.with maxSpeed: 26 @20:43:45.06 SS.seek 25ml @20:44:09.81 Stroke completed -- waiting for pressure to equalize ... @20:44:15.63 Intake.select :close @20:44:15.79 SSV.dial :exhaust @20:44:16.55 Exhaust.select :open @20:44:16.66 SS.reconfigure SSexhale @20:44:16.71 SS.seek 0ml @20:44:25.79 Exhaust.select :close @20:44:25.91 SSV.dial CBV @20:44:25.94 CBV.dial SSV @20:44:26.73 Allowing 130s for 25.0ml of remaining 550.0ml @20:44:26.74 Intake.select :open @20:44:28.92 Intake @35.0psia, vacuum from 20.0 to 24.0psi @20:44:28.94 SS.reconfigure SSinhale.with maxSpeed: 26 @20:44:28.99 SS.seek 25ml @20:45:36.51 Stroke completed -- waiting for pressure to equalize ... @20:45:42.91 Intake.select :close @20:45:43.08 SSV.dial :exhaust @20:45:43.82 Exhaust.select :open @20:45:43.93 SS.reconfigure SSexhale @20:45:43.98 SS.seek 0ml @20:45:52.87 Exhaust.select :close @20:45:52.99 SSV.dial CBV @20:45:53.02 CBV.dial SSV @20:45:53.79 Allowing 130s for 25.0ml of remaining 525.0ml @20:45:53.80 Intake.select :open @20:45:55.98 Intake @35.0psia, vacuum from 20.0 to 24.0psi @20:45:56.00 SS.reconfigure SSinhale.with maxSpeed: 26 @20:45:56.05 SS.seek 25ml @20:46:06.77 AFV.select :carrierSolution @20:46:06.88 delay 2 seconds @20:46:08.91 AS.reconfigure ASconfig @20:46:08.96 AS.seek 1ml @20:46:56.99 delay 1 second @20:46:58.00 AFV.select :holdingCoil @20:46:58.11 delay 1 second @20:46:59.13 ASV.dial :wasteD @20:47:00.16 AS.seek 0ml @20:47:03.84 Stroke completed -- waiting for pressure to equalize ... @20:47:10.83 Intake.select :close @20:47:10.99 SSV.dial :exhaust @20:47:12.28 Exhaust.select :open @20:47:12.42 SS.reconfigure SSexhale @20:47:12.47 SS.seek 0ml @20:47:21.35 Exhaust.select :close @20:47:21.50 SSV.dial CBV @20:47:21.53 CBV.dial SSV @20:47:22.30 Allowing 130s for 25.0ml of remaining 500.0ml @20:47:22.31 Intake.select :open @20:47:24.50 Intake @35.0psia, vacuum from 20.0 to 24.0psi @20:47:24.52 SS.reconfigure SSinhale.with maxSpeed: 26 @20:47:24.57 SS.seek 25ml @20:47:57.94 Priming Eluate @20:47:57.95 AFV.select :carrierSolution @20:47:58.06 delay 2 seconds @20:48:00.09 AS.seek 0.5ml @20:48:24.02 Vacuum too high -- waiting up to 40s @20:48:26.17 SS.reconfigure SSinhale.with maxSpeed: 23 @20:48:26.23 SS.seek 25ml @20:48:29.16 delay 1 second @20:48:30.18 AFV.select :holdingCoil @20:48:30.29 ATV.dial ASV @20:48:30.30 ASV.dial :SPErecovery @20:48:30.85 Swish #1 of 2 @20:48:30.94 AS.reconfigure ASslow0 @20:48:30.99 AS.seek 0.6ml @20:48:35.87 Stroke completed -- waiting for pressure to equalize ... @20:48:41.44 AS.seek 0.5ml @20:48:41.78 Intake.select :close @20:48:41.94 SSV.dial :exhaust @20:48:42.69 Exhaust.select :open @20:48:42.81 SS.reconfigure SSexhale @20:48:42.86 SS.seek 0ml @20:48:51.65 Exhaust.select :close @20:48:51.77 SSV.dial CBV @20:48:51.80 CBV.dial SSV @20:48:51.85 Swish #2 of 2 @20:48:51.97 AS.seek 0.6ml @20:48:52.62 Allowing 130s for 25.0ml of remaining 475.0ml @20:48:52.63 Intake.select :open @20:48:54.81 Intake @35.2psia, vacuum from 20.0 to 24.0psi @20:48:54.83 SS.reconfigure SSinhale.with maxSpeed: 23 @20:48:54.88 SS.seek 25ml @20:49:02.37 AS.seek 0.5ml @20:49:12.75 delay 3 seconds @20:49:15.83 AS.reconfigure ASslow1 @20:49:15.88 AS.seek 0.684ml @20:49:53.17 delay 3 seconds @20:49:56.20 Eluate primed @20:49:56.21 ATV.dial :waste2 @20:49:56.78 AS.reconfigure ASnorm @20:49:56.83 AS.seek 0ml @20:50:11.37 Stroke completed -- waiting for pressure to equalize ... @20:50:17.69 Intake.select :close @20:50:17.86 SSV.dial :exhaust @20:50:18.62 Exhaust.select :open @20:50:18.73 SS.reconfigure SSexhale @20:50:18.78 SS.seek 0ml @20:50:27.89 Exhaust.select :close @20:50:28.01 SSV.dial CBV @20:50:28.04 CBV.dial SSV @20:50:28.81 Allowing 130s for 25.0ml of remaining 450.0ml @20:50:28.82 Intake.select :open @20:50:31.01 Intake @34.8psia, vacuum from 20.0 to 24.0psi @20:50:31.03 SS.reconfigure SSinhale.with maxSpeed: 23 @20:50:31.07 SS.seek 25ml @20:50:31.65 SPE.coast @20:50:31.74 ASV.dial :SPErecovery @20:50:31.76 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0120h1425ml.pcr @20:50:31.78 Building PCR Slug from ASV with MM2 mix, PP4 primer @20:50:31.79 Priming with carrier @20:50:31.80 ATV.dial ARV @20:50:32.71 ARV.dial :carrier @20:50:33.07 AS.seek 0.5ml @20:50:58.59 AFV.select :carrierSolution @20:50:58.71 delay 2 seconds @20:51:00.74 AS.reconfigure ASconfig @20:51:00.79 AS.seek 1ml @20:51:29.85 delay 1 second @20:51:30.86 AFV.select :holdingCoil @20:51:30.97 delay 1 second @20:51:31.99 ATV.dial :waste @20:51:33.38 AS.reconfigure ASnorm @20:51:33.44 AS.seek :empty @20:51:47.31 Stroke completed -- waiting for pressure to equalize ... @20:51:54.34 Intake.select :close @20:51:54.49 SSV.dial :exhaust @20:51:55.23 Exhaust.select :open @20:51:55.35 SS.reconfigure SSexhale @20:51:55.40 SS.seek 0ml @20:52:04.56 Exhaust.select :close @20:52:04.68 SSV.dial CBV @20:52:04.71 CBV.dial SSV @20:52:05.49 Allowing 130s for 25.0ml of remaining 425.0ml @20:52:05.50 Intake.select :open @20:52:07.68 Intake @35.2psia, vacuum from 20.0 to 24.0psi @20:52:07.70 SS.reconfigure SSinhale.with maxSpeed: 23 @20:52:07.75 SS.seek 25ml @20:52:24.27 AFV.select :carrierSolution @20:52:24.39 delay 2 seconds @20:52:26.42 AS.reconfigure ASconfig @20:52:26.47 AS.seek 0.5ml @20:52:55.57 delay 1 second @20:52:56.58 AFV.select :holdingCoil @20:52:56.69 Building Slug @20:52:56.70 ATV.dial :ARV @20:52:58.19 delay 2 seconds @20:53:00.21 ARV.dial :air1 @20:53:00.61 delay 2 seconds @20:53:02.69 AS.reconfigure ASslow2 @20:53:02.75 AS.seek 0.506ml @20:53:08.94 delay 2 seconds @20:53:10.96 ARV.dial :MM2 @20:53:12.38 delay 2 seconds @20:53:14.41 Adding MM2 @20:53:14.48 AS.seek 0.524ml @20:53:18.86 Vacuum too high -- waiting up to 40s @20:53:21.04 Intake @35.1psia, vacuum from 20.0 to 24.0psi @20:53:21.06 SS.reconfigure SSinhale.with maxSpeed: 21 @20:53:21.11 SS.seek 25ml @20:53:26.56 Stroke completed -- waiting for pressure to equalize ... @20:53:32.36 Intake.select :close @20:53:32.52 SSV.dial :exhaust @20:53:32.85 delay 25 seconds @20:53:33.27 Exhaust.select :open @20:53:33.38 SS.reconfigure SSexhale @20:53:33.43 SS.seek 0ml @20:53:42.37 Exhaust.select :close @20:53:42.49 SSV.dial CBV @20:53:42.52 CBV.dial SSV @20:53:43.29 Allowing 130s for 25.0ml of remaining 400.0ml @20:53:43.30 Intake.select :open @20:53:45.49 Intake @35.0psia, vacuum from 20.0 to 24.0psi @20:53:45.51 SS.reconfigure SSinhale.with maxSpeed: 21 @20:53:45.56 SS.seek 25ml @20:53:57.87 ARV.dial :PP4 @20:53:58.27 Adding PP4 @20:53:58.29 delay 2 seconds @20:54:00.37 AS.seek 0.53ml @20:54:06.57 delay 10 seconds @20:54:16.59 ARV.dial :air3 @20:54:16.99 adding ARV air @20:54:17.00 delay 2 seconds @20:54:19.09 AS.seek 0.536ml @20:54:25.49 delay 5 seconds @20:54:30.51 ARV.dial :carrier @20:54:31.72 delay 7 seconds @20:54:38.81 AS.seek 0.594ml @20:55:08.90 Stroke completed -- waiting for pressure to equalize ... @20:55:16.57 Intake.select :close @20:55:16.72 SSV.dial :exhaust @20:55:17.48 Exhaust.select :open @20:55:17.59 SS.reconfigure SSexhale @20:55:17.64 SS.seek 0ml @20:55:26.86 Exhaust.select :close @20:55:26.98 SSV.dial CBV @20:55:27.01 CBV.dial SSV @20:55:27.79 Allowing 130s for 25.0ml of remaining 375.0ml @20:55:27.80 Intake.select :open @20:55:29.99 Intake @35.1psia, vacuum from 20.0 to 24.0psi @20:55:30.01 SS.reconfigure SSinhale.with maxSpeed: 21 @20:55:30.05 SS.seek 25ml @20:55:37.57 delay 5 seconds @20:55:42.59 ATV.dial :ASV @20:55:42.97 delay 2 seconds @20:55:45.06 AS.seek 0.6ml @20:55:51.36 delay 4 seconds @20:55:55.38 ATV.dial ARV @20:55:55.89 delay 2 seconds @20:55:57.91 ARV.dial :carrier @20:55:58.42 delay 2 seconds @20:56:00.60 AS.seek 0.65ml @20:56:51.24 delay 4 seconds @20:56:53.53 Stroke completed -- waiting for pressure to equalize ... @20:56:55.25 ATV.dial PCR @20:56:55.82 ASV.dial :waste1 @20:56:56.76 ARV.dial :air1 @20:56:57.14 Positioning Slug by 0.324ml @20:56:57.23 AS.seek 0.326ml @20:57:02.55 Intake.select :close @20:57:02.71 SSV.dial :exhaust @20:57:03.46 Exhaust.select :open @20:57:03.57 SS.reconfigure SSexhale @20:57:03.62 SS.seek 0ml @20:57:12.89 Exhaust.select :close @20:57:13.01 SSV.dial CBV @20:57:13.04 CBV.dial SSV @20:57:13.80 Allowing 130s for 25.0ml of remaining 350.0ml @20:57:13.81 Intake.select :open @20:57:16.00 Intake @35.4psia, vacuum from 20.0 to 24.0psi @20:57:16.02 SS.reconfigure SSinhale.with maxSpeed: 21 @20:57:16.07 SS.seek 25ml @20:58:08.89 Vacuum too high -- waiting up to 40s @20:58:11.00 Intake @35.6psia, vacuum from 20.0 to 24.0psi @20:58:11.02 SS.reconfigure SSinhale.with maxSpeed: 19 @20:58:11.07 SS.seek 25ml @20:58:44.84 Stroke completed -- waiting for pressure to equalize ... @20:58:53.11 Intake.select :close @20:58:53.27 SSV.dial :exhaust @20:58:54.01 Exhaust.select :open @20:58:54.64 SS.reconfigure SSexhale @20:58:54.78 SS.seek 0ml @20:59:03.92 Exhaust.select :close @20:59:04.04 SSV.dial CBV @20:59:04.07 CBV.dial SSV @20:59:04.85 Allowing 130s for 25.0ml of remaining 325.0ml @20:59:04.86 Intake.select :open @20:59:07.04 Intake @34.9psia, vacuum from 20.0 to 24.0psi @20:59:07.06 SS.reconfigure SSinhale.with maxSpeed: 19 @20:59:07.11 SS.seek 25ml @21:00:39.62 Stroke completed -- waiting for pressure to equalize ... @21:00:48.58 Intake.select :close @21:00:48.74 SSV.dial :exhaust @21:00:49.49 Exhaust.select :open @21:00:49.60 SS.reconfigure SSexhale @21:00:49.65 SS.seek 0ml @21:00:58.75 Exhaust.select :close @21:00:58.87 SSV.dial CBV @21:00:58.90 CBV.dial SSV @21:00:59.68 Allowing 130s for 25.0ml of remaining 300.0ml @21:00:59.69 Intake.select :open @21:01:01.88 Intake @35.0psia, vacuum from 20.0 to 24.0psi @21:01:01.90 SS.reconfigure SSinhale.with maxSpeed: 19 @21:01:01.94 SS.seek 25ml @21:02:23.38 delay 5 seconds @21:02:28.40 ATV.dial ARV @21:02:29.24 ASV.dial :PCRlock @21:02:29.61 delay 5 seconds @21:02:34.09 Stroke completed -- waiting for pressure to equalize ... @21:02:34.72 Running Tt1 Profile60C300 thermocycling @21:02:34.74 PCR.program Profile60C300 @21:02:34.90 Profile60C300 should complete in < 1:45:21 @21:02:34.91 PCR.run 2:16:41.25 @21:02:44.36 Intake.select :close @21:02:44.52 SSV.dial :exhaust @21:02:45.27 Exhaust.select :open @21:02:45.38 SS.reconfigure SSexhale @21:02:45.43 SS.seek 0ml @21:02:54.32 Exhaust.select :close @21:02:54.44 SSV.dial CBV @21:02:54.47 CBV.dial SSV @21:02:55.82 Allowing 130s for 25.0ml of remaining 275.0ml @21:02:55.83 Intake.select :open @21:02:58.01 Intake @34.9psia, vacuum from 20.0 to 24.0psi @21:02:58.03 SS.reconfigure SSinhale.with maxSpeed: 19 @21:02:58.09 SS.seek 25ml @21:04:08.99 Vacuum too high -- waiting up to 40s @21:04:11.66 Intake @35.1psia, vacuum from 20.0 to 24.0psi @21:04:11.68 SS.reconfigure SSinhale.with maxSpeed: 17 @21:04:11.72 SS.seek 25ml @21:04:35.81 Stroke completed -- waiting for pressure to equalize ... @21:04:45.40 Intake.select :close @21:04:45.56 SSV.dial :exhaust @21:04:46.32 Exhaust.select :open @21:04:46.43 SS.reconfigure SSexhale @21:04:46.48 SS.seek 0ml @21:04:55.37 Exhaust.select :close @21:04:55.49 SSV.dial CBV @21:04:55.52 CBV.dial SSV @21:04:56.30 Allowing 130s for 25.0ml of remaining 250.0ml @21:04:56.31 Intake.select :open @21:04:58.49 Intake @35.3psia, vacuum from 20.0 to 24.0psi @21:04:58.51 SS.reconfigure SSinhale.with maxSpeed: 17 @21:04:58.56 SS.seek 25ml @21:06:41.93 Stroke completed -- waiting for pressure to equalize ... @21:06:52.72 Intake.select :close @21:06:52.88 SSV.dial :exhaust @21:06:53.63 Exhaust.select :open @21:06:53.74 SS.reconfigure SSexhale @21:06:53.79 SS.seek 0ml @21:07:02.96 Exhaust.select :close @21:07:03.08 SSV.dial CBV @21:07:03.11 CBV.dial SSV @21:07:03.88 Allowing 130s for 25.0ml of remaining 225.0ml @21:07:03.89 Intake.select :open @21:07:06.07 Intake @35.5psia, vacuum from 20.0 to 24.0psi @21:07:06.09 SS.reconfigure SSinhale.with maxSpeed: 17 @21:07:06.14 SS.seek 25ml @21:08:21.27 Vacuum too high -- waiting up to 40s @21:08:23.87 Intake @35.3psia, vacuum from 20.0 to 24.0psi @21:08:23.89 SS.reconfigure SSinhale.with maxSpeed: 15 @21:08:23.94 SS.seek 25ml @21:08:55.64 Stroke completed -- waiting for pressure to equalize ... @21:09:05.85 Intake.select :close @21:09:06.01 SSV.dial :exhaust @21:09:06.75 Exhaust.select :open @21:09:06.87 SS.reconfigure SSexhale @21:09:06.92 SS.seek 0ml @21:09:15.85 Exhaust.select :close @21:09:15.97 SSV.dial CBV @21:09:16.00 CBV.dial SSV @21:09:16.77 Allowing 130s for 25.0ml of remaining 200.0ml @21:09:16.78 Intake.select :open @21:09:18.97 Intake @35.2psia, vacuum from 20.0 to 24.0psi @21:09:18.99 SS.reconfigure SSinhale.with maxSpeed: 15 @21:09:19.03 SS.seek 25ml @21:11:16.06 Stroke completed -- waiting for pressure to equalize ... @21:11:26.86 SS.reconfigure SSconfig @21:11:26.93 SS.hold @21:11:27.06 Intake.select :close @21:11:27.22 ShallowSampler::Clogged in WCR -- progress too slow after 1325.0ml @21:11:27.23 SSV.dial :exhaust @21:11:27.97 Exhaust.select :open @21:11:28.08 SS.reconfigure SSexhale @21:11:28.13 SS.seek 0ml @21:11:36.96 Exhaust.select :close @21:11:37.08 Sampled 1325.0ml @21:11:37.10 Drying puck before fixing for 4:00 @21:11:37.11 CBV.dial :waste2b @21:11:37.83 delay 1:00 @21:12:37.85 CSV.dial :air @21:12:38.29 CS.reconfigure CSfast @21:12:38.34 CS.seek 10ml @21:13:00.05 Pumping wcr puck from tube 2 up to 55psi... @21:13:00.06 CBV.dial SSV @21:13:01.35 CBV.dial between CSV and waste2b @21:13:02.29 CSV.dial between air and CTV @21:13:02.63 CS.seek 6.683ml @21:13:10.65 CTV.dial CSV,avoiding: :air @21:13:10.68 CSV.dial CTV @21:13:11.46 CS.reconfigure CSpressurize @21:13:11.51 CS.seek 0ml @21:13:28.45 CBV.dial :waste2b @21:13:28.81 CS.reconfigure CSconfig @21:13:28.87 CS.seek :empty @21:13:31.15 delay 1:00 @21:14:31.18 CTV.dial :air,avoiding: :intake @21:14:32.44 Fix #1 of 2 (with rnal) for 2:00 @21:14:32.47 CSV.dial CSR @21:14:32.96 Pulling 2ml of CSR.rnal @21:14:32.97 CSR.select :rnal @21:14:33.10 CS.seek 2ml @21:14:39.18 delay 5 seconds @21:14:44.20 CSR.select :air @21:14:44.33 CS.reconfigure CSfast @21:14:44.39 CS.seek 10ml @21:15:02.09 Pumping wcr puck from tube 2 up to 55psi... @21:15:02.10 CBV.dial SSV @21:15:03.38 CBV.dial between CSV and waste2b @21:15:04.33 CSV.dial between air and CTV @21:15:04.92 CS.seek 6.74ml @21:15:12.90 CTV.dial CSV,avoiding: :air @21:15:12.93 CSV.dial CTV @21:15:14.24 CS.reconfigure CSpressurize @21:15:14.30 CS.seek 0ml @21:15:31.11 delay 2:00 @21:17:31.13 CBV.dial :waste2b @21:17:31.48 CS.reconfigure CSconfig @21:17:31.53 CS.seek :empty @21:17:33.37 delay 1:00 @21:18:33.39 CTV.dial :air,avoiding: :intake @21:18:34.64 Fix #2 of 2 (with rnal) for 2:00 @21:18:34.66 CSV.dial CSR @21:18:35.15 Pulling 2ml of CSR.rnal @21:18:35.16 CSR.select :rnal @21:18:35.29 CS.seek 2ml @21:18:41.59 delay 5 seconds @21:18:46.61 CSR.select :air @21:18:46.74 CS.reconfigure CSfast @21:18:46.79 CS.seek 10ml @21:19:04.25 Pumping wcr puck from tube 2 up to 55psi... @21:19:04.26 CBV.dial SSV @21:19:05.56 CBV.dial between CSV and waste2b @21:19:06.50 CSV.dial between air and CTV @21:19:07.08 CS.seek 6.654ml @21:19:15.59 CTV.dial CSV,avoiding: :air @21:19:15.62 CSV.dial CTV @21:19:16.90 CS.reconfigure CSpressurize @21:19:16.96 CS.seek 0ml @21:19:32.55 delay 2:00 @21:21:32.57 CBV.dial :waste2b @21:21:32.92 CS.reconfigure CSconfig @21:21:32.97 CS.seek :empty @21:21:36.03 delay 1:00 @21:22:36.06 CTV.dial :air,avoiding: :intake @21:22:37.36 Storing wcr puck in Collection Clamp to tube 1 @21:22:37.55 SC.reconfigure SCtight @21:22:37.60 SC.seek 1 @21:22:39.72 SC.reconfigure SCloose @21:22:39.95 Elbow.reconfigure TightElbow @21:22:40.00 Elbow.seek 1 @21:22:41.87 Forearm.reconfigure TightForearm @21:22:41.92 Forearm.seek 1 + 700 counts @21:22:42.93 SE.reconfigure SEpick @21:22:43.01 SE.seek beamBreak @21:22:45.66 SE.configure SEconfig @21:22:45.84 Elbow.reconfigure LimpElbow @21:22:45.89 Forearm.reconfigure LooseForearm @21:22:45.94 Forearm.seek 1 @21:22:46.27 Elbow.hold @21:22:46.34 Elbow.reconfigure TightElbow @21:22:46.45 SE.seek 4.08 Pucks below Top Plate @21:22:46.94 Forearm.force -20 @21:22:46.98 Forearm.seek 1 + 321 counts @21:22:47.35 Forearm.reconfigure TightForearm @21:22:47.41 Forearm.seek 1 + 700 counts @21:22:47.73 SE.seek 5.08 Pucks below Top Plate @21:22:48.33 Forearm.reconfigure LooseForearm @21:22:48.38 Forearm.seek :retract @21:22:49.33 Elbow.seek CC @21:22:51.00 Elbow.reconfigure LooseElbow @21:22:51.11 Forearm.seek CC @21:22:52.09 Hand.close @21:22:52.78 CC.seek :open @21:22:57.89 Forearm.seek :retract @21:22:58.89 Elbow.reconfigure TightElbow @21:22:58.94 Elbow.seek 1 @21:23:00.88 Elbow.reconfigure LimpElbow @21:23:00.94 Forearm.seek 1 @21:23:01.97 Elbow.hold @21:23:02.05 Elbow.reconfigure TightElbow @21:23:02.10 Hand.open @21:23:02.92 Forearm.force -20 @21:23:02.97 Forearm.seek 1 + 346 counts @21:23:03.30 Forearm.reconfigure TightForearm @21:23:03.36 Forearm.seek 1 + 700 counts @21:23:03.74 SE.seek :down @21:23:04.89 Forearm.reconfigure LooseForearm @21:23:04.94 Forearm.seek :retract @21:23:06.01 SE.coast @21:23:06.05 SC.coast @21:23:06.10 Starting finalPurge @21:23:06.11 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:23:06.19 Hand.open @21:23:06.55 Elbow.seek FlushPuck::Garage @21:23:08.59 Elbow.reconfigure LooseElbow @21:23:08.70 Forearm.seek :garage @21:23:09.71 Hand.close @21:23:10.42 Forearm.seek :retract @21:23:11.43 Elbow.reconfigure TightElbow @21:23:11.48 Elbow.seek CC @21:23:14.45 Elbow.reconfigure LooseElbow @21:23:14.56 Forearm.reconfigure TightForearm @21:23:14.61 Forearm.seek CC @21:23:15.85 CC.seek :closed @21:23:20.10 Collection Clamp onSpring/offSpring for 6/32 samples, on/off torque 1.69 @21:23:20.19 Hand.open @21:23:20.86 Forearm.reconfigure LooseForearm @21:23:20.91 Forearm.seek :retract @21:23:21.94 Flushing the Collection Valve::Manifold @21:23:22.08 CSV.dial CSR @21:23:23.17 Pulling 5ml of CSR.flush @21:23:23.18 CSR.select :flush @21:23:23.31 CS.seek 5ml @21:23:37.50 delay 5 seconds @21:23:42.52 CSR.select :air @21:23:42.69 CS.reconfigure CSfast @21:23:42.75 CS.seek 7.5ml @21:23:49.05 CSV.dial :waste @21:23:50.25 CS.reconfigure CSconfig @21:23:50.30 CS.seek 0ml @21:24:10.47 Flushing lower manifold's waste1 port @21:24:10.63 CSV.dial :flush @21:24:11.06 CS.reconfigure CSfast @21:24:11.11 CS.seek 5ml @21:24:22.41 delay 5 seconds @21:24:27.43 CSV.dial :air @21:24:27.95 CS.seek 7ml @21:24:33.16 CSV.dial CTV @21:24:33.20
CTV.dial CSV @21:24:34.50 CBV.dial :waste1 @21:24:35.22 CS.seek 0ml @21:24:50.66 Flushing lower manifold's waste2b port @21:24:50.79 CSV.dial :flush @21:24:51.51 CS.seek 5ml @21:25:02.91 delay 5 seconds @21:25:07.92 CSV.dial :air @21:25:08.46 CS.seek 7ml @21:25:13.66 CSV.dial CTV @21:25:13.70
CTV.dial CSV @21:25:14.14 CBV.dial :waste2b @21:25:14.86 CS.seek 0ml @21:25:30.16 Flushing upper manifold @21:25:30.29 CSV.dial :flush @21:25:31.00 CS.seek 5ml @21:25:42.35 delay 5 seconds @21:25:47.36 CSV.dial :air @21:25:47.90 CS.seek 7ml @21:25:53.09 CSV.dial CBV @21:25:53.12
CBV.dial CSV @21:25:54.13 CTV.dial :air @21:25:55.38 CS.seek 0ml @21:26:10.75 delay 7 seconds @21:26:17.77 CS.seek 3ml @21:26:24.99 CSV.dial :waste @21:26:25.44 CS.seek 0ml @21:26:32.81 CSV.dial :flush @21:26:33.25 CS.seek 3ml @21:26:40.60 delay 5 seconds @21:26:45.61 CSV.dial :air @21:26:46.13 CS.seek 5.001ml @21:26:51.30 CSV.dial :air @21:26:51.33 CS.seek 0ml @21:27:02.78 delay 7 seconds @21:27:09.80 CS.seek 3ml @21:27:16.99 CSV.dial :waste @21:27:18.29 CS.seek 0ml @21:27:25.47 Disinfecting external Intake with mfbkill @21:27:25.61 CSV.dial CSR @21:27:26.89 Pulling 8ml of CSR.mfbkill @21:27:26.90 CSR.select :mfbkill @21:27:27.03 CS.seek 8ml @21:27:44.69 delay 5 seconds @21:27:49.70 CSR.select :air @21:27:49.84 CS.seek 10ml @21:27:55.10 CSV.dial CBV @21:27:55.13
CBV.dial CSV @21:27:56.11 CTV.dial :intake @21:27:56.83 Intake.select :open @21:27:56.95 CS.seek 2ml @21:28:15.21 Intake.select :close @21:28:15.33 CTV.dial :air @21:28:16.03 CS.reconfigure CSconfig @21:28:16.08 CS.seek 0ml @21:28:22.16 Flushing lower manifold's waste1 port @21:28:22.17 CSV.dial CSR @21:28:23.19 Pulling 4ml of CSR.mfbkill @21:28:23.20 CSR.select :mfbkill @21:28:23.33 CS.seek 4ml @21:28:34.53 delay 5 seconds @21:28:39.55 CSR.select :air @21:28:39.68 CS.reconfigure CSfast @21:28:39.73 CS.seek 10ml @21:28:53.07 CSV.dial CTV @21:28:53.10
CTV.dial CSV @21:28:54.42 CBV.dial :waste1 @21:28:54.87 CS.seek 0ml @21:29:16.22 Flushing lower manifold's waste2b port @21:29:16.23 CSV.dial CSR @21:29:16.74 Pulling 4ml of CSR.mfbkill @21:29:16.75 CSR.select :mfbkill @21:29:16.88 CS.reconfigure CSconfig @21:29:16.92 CS.seek 4ml @21:29:28.16 delay 5 seconds @21:29:33.17 CSR.select :air @21:29:33.30 CS.reconfigure CSfast @21:29:33.35 CS.seek 10ml @21:29:46.79 CSV.dial CTV @21:29:46.82
CTV.dial CSV @21:29:47.29 CBV.dial :waste2b @21:29:48.03 CS.seek 0ml @21:30:09.50 Killing upper manifold @21:30:09.51 CSV.dial CSR @21:30:10.02 Pulling 4ml of CSR.mfbkill @21:30:10.03 CSR.select :mfbkill @21:30:10.16 CS.reconfigure CSconfig @21:30:10.20 CS.seek 4ml @21:30:21.35 delay 5 seconds @21:30:26.37 CSR.select :air @21:30:26.50 CS.reconfigure CSfast @21:30:26.55 CS.seek 10ml @21:30:39.85 CSV.dial CBV @21:30:39.88
CBV.dial CSV @21:30:40.87 CTV.dial :air @21:30:42.12 CS.seek 0ml @21:31:03.44 delay 7 seconds @21:31:10.46 CS.seek 3ml @21:31:17.62 CSV.dial :waste @21:31:18.06 CS.seek 0ml @21:31:25.44 Flushing upper manifold @21:31:26.16 CSV.dial :flush @21:31:26.61 CS.seek 5ml @21:31:37.91 delay 5 seconds @21:31:42.92 CSV.dial :air @21:31:43.43 CS.seek 7.001ml @21:31:48.69 CSV.dial CBV @21:31:48.72
CBV.dial CSV @21:31:49.69 CTV.dial :air @21:31:49.71 CS.seek 0ml @21:32:05.01 delay 7 seconds @21:32:12.03 CS.seek 3ml @21:32:19.37 CSV.dial :waste @21:32:19.83 CS.seek 0ml @21:32:27.22 CSV.dial :flush @21:32:27.67 CS.seek 3ml @21:32:35.13 delay 5 seconds @21:32:40.14 CSV.dial :air @21:32:40.70 CS.seek 5ml @21:32:45.85 CSV.dial :air @21:32:45.87 CS.seek 0ml @21:32:57.04 delay 7 seconds @21:33:04.06 CS.seek 3ml @21:33:11.22 CSV.dial :waste @21:33:11.93 CS.seek 0ml @21:33:19.10 Flushing lower manifold's waste1 port @21:33:19.24 CSV.dial :flush @21:33:19.70 CS.seek 5ml @21:33:31.04 delay 5 seconds @21:33:36.05 CSV.dial :air @21:33:36.58 CS.seek 7.001ml @21:33:41.76 CSV.dial CTV @21:33:41.79
CTV.dial CSV @21:33:43.09 CBV.dial :waste1 @21:33:43.56 CS.seek 0ml @21:33:58.85 Flushing lower manifold's waste2b port @21:33:58.99 CSV.dial :flush @21:33:59.70 CS.seek 5ml @21:34:10.91 delay 5 seconds @21:34:15.92 CSV.dial :air @21:34:16.45 CS.seek 7ml @21:34:21.73 CSV.dial CTV @21:34:21.75
CTV.dial CSV @21:34:22.20 CBV.dial :waste2b @21:34:22.94 CS.seek 0ml @21:34:38.20 Disinfecting external Intake with kill @21:34:38.33 CSV.dial CSR @21:34:38.83 Pulling 8ml of CSR.kill @21:34:38.84 CSR.select :kill @21:34:38.97 CS.seek 8ml @21:34:56.20 delay 5 seconds @21:35:01.21 CSR.select :air @21:35:01.35 CS.seek 10ml @21:35:06.74 CSV.dial CBV @21:35:06.77
CBV.dial CSV @21:35:07.90 CTV.dial :intake @21:35:08.61 Intake.select :open @21:35:08.74 CS.seek 2ml @21:35:26.87 Intake.select :close @21:35:26.99 CTV.dial :air @21:35:27.71 CS.reconfigure CSconfig @21:35:27.76 CS.seek 0ml @21:35:33.87 CSV.dial CSR @21:35:34.88 Pulling 5ml of CSR.flush @21:35:34.89 CSR.select :flush @21:35:35.02 CS.seek 5ml @21:35:49.10 delay 5 seconds @21:35:54.12 CSR.select :air @21:35:54.26 CS.seek 10ml @21:36:07.97 CSV.dial CBV @21:36:08.95 CS.seek 0ml @21:36:35.42 Garaging FlushPuck in Collection Clamp @21:36:35.50 Elbow.reconfigure TightElbow @21:36:35.55 Elbow.seek CC @21:36:35.80 Elbow.reconfigure LooseElbow @21:36:35.91 Forearm.seek CC @21:36:36.89 Hand.close @21:36:37.55 CC.seek :open @21:36:41.92 Forearm.seek :retract @21:36:42.93 Elbow.reconfigure TightElbow @21:36:42.98 Elbow.seek :garage @21:36:46.09 Elbow.reconfigure LooseElbow @21:36:46.19 Forearm.reconfigure TightForearm @21:36:46.24 Forearm.seek :garage @21:36:47.39 Hand.open @21:36:48.12 Forearm.reconfigure LooseForearm @21:36:48.17 Forearm.seek :retract @21:36:49.19 Starting initialPurge @21:36:49.20 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @21:36:49.28 Hand.open @21:36:49.62 Elbow.reconfigure TightElbow @21:36:49.66 Elbow.seek FlushPuck::Garage @21:36:49.80 Elbow.reconfigure LooseElbow @21:36:49.91 Forearm.seek :garage @21:36:50.93 Hand.close @21:36:51.68 Forearm.seek :retract @21:36:52.70 Elbow.reconfigure TightElbow @21:36:52.75 Elbow.seek CC @21:36:55.60 Elbow.reconfigure LooseElbow @21:36:55.70 Forearm.reconfigure TightForearm @21:36:55.75 Forearm.seek CC @21:36:56.99 CC.seek :closed @21:37:01.24 Collection Clamp onSpring/offSpring for 5/32 samples, on/off torque 1.69 @21:37:01.33 Hand.open @21:37:02.00 Forearm.reconfigure LooseForearm @21:37:02.04 Forearm.seek :retract @21:37:03.60 Flushing the Collection Valve::Manifold @21:37:03.78 CSV.dial CSR @21:37:04.80 Pulling 5ml of CSR.flush @21:37:04.81 CSR.select :flush @21:37:04.94 CS.seek 5ml @21:37:18.73 delay 5 seconds @21:37:23.75 CSR.select :air @21:37:23.93 CS.reconfigure CSfast @21:37:23.98 CS.seek 7.5ml @21:37:30.19 CSV.dial :waste @21:37:31.42 CS.reconfigure CSconfig @21:37:31.47 CS.seek 0ml @21:37:51.64 Purging external Intake @21:37:51.77 CSV.dial :flush @21:37:52.23 CS.reconfigure CSfast @21:37:52.28 CS.seek 10ml @21:38:13.67 delay 5 seconds @21:38:18.69 CSV.dial CBV @21:38:18.72
CBV.dial CSV @21:38:19.45 CTV.dial :intake @21:38:20.16 Intake.select :open @21:38:20.29 CS.seek 0ml @21:38:42.31 Intake.select :close @21:38:42.43 CTV.dial :air @21:38:43.15 Flushing upper manifold @21:38:43.28 CSV.dial :flush @21:38:43.99 CS.seek 5ml @21:38:55.23 delay 5 seconds @21:39:00.24 CSV.dial :air @21:39:00.75 CS.seek 7ml @21:39:05.93 CSV.dial CBV @21:39:05.95
CBV.dial CSV @21:39:06.93 CTV.dial :air @21:39:06.95 CS.seek 0ml @21:39:22.29 delay 7 seconds @21:39:29.31 CS.seek 3ml @21:39:36.50 CSV.dial :waste @21:39:36.95 CS.seek 0ml @21:39:44.38 CSV.dial :flush @21:39:44.82 CS.seek 3ml @21:39:52.07 delay 5 seconds @21:39:57.08 CSV.dial :air @21:39:57.60 CS.seek 5ml @21:40:02.76 CSV.dial :air @21:40:02.79 CS.seek 0ml @21:40:14.17 delay 7 seconds @21:40:21.19 CS.seek 3ml @21:40:28.47 CSV.dial :waste @21:40:29.18 CS.seek 0ml @21:40:36.48 Garaging FlushPuck in Collection Clamp @21:40:36.56 Elbow.reconfigure TightElbow @21:40:36.61 Elbow.seek CC @21:40:36.84 Elbow.reconfigure LooseElbow @21:40:36.95 Forearm.seek CC @21:40:37.92 Hand.close @21:40:38.56 CC.seek :open @21:40:42.91 Forearm.seek :retract @21:40:43.92 Elbow.reconfigure TightElbow @21:40:43.97 Elbow.seek :garage @21:40:46.98 Elbow.reconfigure LooseElbow @21:40:47.09 Forearm.reconfigure TightForearm @21:40:47.14 Forearm.seek :garage @21:40:48.92 Hand.open @21:40:49.63 Forearm.reconfigure LooseForearm @21:40:49.68 Forearm.seek :retract @21:40:50.74 Completed [mbaPhase] begun 17:56:56 @21:40:50.76 Preparing to sleep until 14:00 2-oct-18 @21:40:50.80 deferred until MFBPCR finish @21:40:50.82
Waiting for MFBPCR til 14:00 2-oct-18 @21:40:50.86 Continuing at 14:00CEST02-Oct-18 @21:40:50.93 GoodNews.email "Then, in tube 2: coreNEG\n/home/esp/esp2/mission/18tundeployv2.rb:110\n{1 => 6, 2 => 16}\nCan@21:40:50, 15.8C, 67% humidity, 14.4psia, 14.940V, 0.714A, 0.714A avg, 10.67W",Subject: "Waiting until 14:00CEST02-Oct-18" @22:24:58.60 PCR.off @22:25:00.18 PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0120h1425ml.pcr @22:25:00.25 Cleaning PCR @22:25:00.26 AFV.select :carrierSolution @22:25:00.37 delay 2 seconds @22:25:02.40 AS.reconfigure ASconfig @22:25:02.45 AS.seek 0.5ml @22:25:12.77 delay 1 second @22:25:13.78 AFV.select :holdingCoil @22:25:13.89 ASV.dial :waste1 @22:25:14.27 ATV.dial :air3 @22:25:15.45 AS.reconfigure ASslow1 @22:25:15.50 AS.seek 0.51ml @22:25:17.79 delay 2 seconds @22:25:19.81 ATV.dial :bleach @22:25:20.41 AS.seek 0.81ml @22:26:20.98 delay 3 seconds @22:26:24.00 ATV.dial :air3 @22:26:24.52 delay 3 seconds @22:26:27.61 AS.seek 0.835ml @22:26:32.91 delay 3 seconds @22:26:35.93 ATV.dial :bleach @22:26:36.53 AS.seek 0.82ml @22:26:39.84 delay 3 seconds @22:26:42.86 ARV.dial :waste @22:26:43.46 delay 3 seconds @22:26:46.48 ATV.dial :ARV @22:26:47.41 AS.reconfigure ASconfig @22:26:47.47 AS.seek 0.47ml @22:27:07.87 AFV.select :carrierSolution @22:27:07.99 delay 2 seconds @22:27:10.02 AS.seek 1ml @22:27:40.79 delay 1 second @22:27:41.80 AFV.select :holdingCoil @22:27:41.91 delay 1 second @22:27:42.94 AS.reconfigure ASfast @22:27:42.99 AS.seek 0ml @22:28:10.33 AFV.select :carrierSolution @22:28:10.45 delay 2 seconds @22:28:12.48 AS.reconfigure ASconfig @22:28:12.53 AS.seek 1ml @22:29:10.31 delay 1 second @22:29:11.32 AFV.select :holdingCoil @22:29:11.43 delay 1 second @22:29:12.46 AS.reconfigure ASfast @22:29:12.51 AS.seek 0ml @22:29:39.86 AFV.select :carrierSolution @22:29:39.98 delay 2 seconds @22:29:42.51 AS.reconfigure ASconfig @22:29:42.63 AS.seek 1ml @22:30:40.43 delay 1 second @22:30:41.44 AFV.select :holdingCoil @22:30:41.55 delay 1 second @22:30:42.58 AS.reconfigure ASfast @22:30:42.63 AS.seek 0ml @22:31:09.98 ARV.dial between carrier and air1 @22:31:10.48 ATV.dial :air3 @22:31:11.67 AS.reconfigure ASslow1 @22:31:11.72 AS.seek 0.01ml @22:31:13.98 delay 2 seconds @22:31:15.99 ATV.dial :bleach @22:31:16.59 AS.seek 0.81ml @22:33:57.71 delay 3 seconds @22:34:00.73 ATV.dial :air3 @22:34:01.26 delay 3 seconds @22:34:04.35 AS.seek 0.835ml @22:34:09.68 delay 3 seconds @22:34:12.70 ATV.dial :bleach @22:34:13.30 AS.seek 0.82ml @22:34:16.60 delay 3 seconds @22:34:19.63 ATV.dial PCR @22:34:20.01 AS.reconfigure ASconfig @22:34:20.06 AS.seek :empty @22:35:07.52 PCR.program :bleachProfile @22:35:07.58 PCR program should complete in < 2:45 @22:35:07.59 PCR.run 10:00 @22:38:15.47 PCR.off @22:38:15.49 AFV.select :carrierSolution @22:38:15.61 delay 2 seconds @22:38:17.64 AS.seek 1ml @22:39:15.46 delay 1 second @22:39:16.47 AFV.select :holdingCoil @22:39:16.58 delay 1 second @22:39:17.61 AS.reconfigure ASfast @22:39:17.66 AS.seek 0ml @22:39:45.00 delay 3 seconds @22:39:48.02 AFV.select :carrierSolution @22:39:48.13 delay 2 seconds @22:39:50.16 AS.reconfigure ASconfig @22:39:50.21 AS.seek 1ml @22:40:48.00 delay 1 second @22:40:49.01 AFV.select :holdingCoil @22:40:49.12 delay 1 second @22:40:50.15 AS.reconfigure ASfast @22:40:50.20 AS.seek 0ml @22:41:17.54 delay 3 seconds @22:41:20.56 AFV.select :carrierSolution @22:41:20.67 delay 2 seconds @22:41:22.70 AS.reconfigure ASconfig @22:41:22.76 AS.seek 1ml @22:42:20.53 delay 1 second @22:42:21.55 AFV.select :holdingCoil @22:42:21.66 delay 1 second @22:42:22.69 AS.reconfigure ASfast @22:42:22.74 AS.seek 0ml @22:42:50.09 delay 3 seconds @22:42:53.11 AFV.select :carrierSolution @22:42:53.25 delay 2 seconds @22:42:55.28 AS.reconfigure ASconfig @22:42:55.33 AS.seek 1ml @22:43:53.16 delay 1 second @22:43:54.18 AFV.select :holdingCoil @22:43:54.29 delay 1 second @22:43:55.32 AS.reconfigure ASfast @22:43:55.37 AS.seek 0ml @22:44:22.73 delay 3 seconds @22:44:25.75 AFV.select :carrierSolution @22:44:25.86 delay 2 seconds @22:44:27.89 AS.reconfigure ASconfig @22:44:27.95 AS.seek 1ml @22:45:25.75 delay 1 second @22:45:26.76 AFV.select :holdingCoil @22:45:26.87 delay 1 second @22:45:27.90 AS.reconfigure ASnorm @22:45:27.95 AS.seek 0ml @22:46:18.69 Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0122h1425ml.pcr @22:46:18.71 Building PCR Slug from ASV with MM2 mix, PP3 primer @22:46:18.72 Priming with carrier @22:46:18.73 ATV.dial ARV @22:46:19.39 ARV.dial :carrier @22:46:19.74 AS.seek 0.5ml @22:46:45.28 AFV.select :carrierSolution @22:46:45.40 delay 2 seconds @22:46:47.43 AS.reconfigure ASconfig @22:46:47.48 AS.seek 1ml @22:47:16.50 delay 1 second @22:47:17.51 AFV.select :holdingCoil @22:47:17.62 delay 1 second @22:47:18.64 ATV.dial :waste @22:47:20.15 AS.reconfigure ASnorm @22:47:20.20 AS.seek :empty @22:48:10.96 AFV.select :carrierSolution @22:48:11.08 delay 2 seconds @22:48:13.11 AS.reconfigure ASconfig @22:48:13.16 AS.seek 0.5ml @22:48:42.90 delay 1 second @22:48:43.91 AFV.select :holdingCoil @22:48:44.02 Building Slug @22:48:44.03 ATV.dial :ARV @22:48:45.53 delay 2 seconds @22:48:47.55 ARV.dial :air1 @22:48:47.95 delay 2 seconds @22:48:50.04 AS.reconfigure ASslow2 @22:48:50.09 AS.seek 0.506ml @22:48:56.45 delay 2 seconds @22:48:58.47 ARV.dial :MM2 @22:48:59.86 delay 2 seconds @22:49:01.89 Adding MM2 @22:49:01.97 AS.seek 0.524ml @22:49:20.28 delay 25 seconds @22:49:45.30 ARV.dial :PP3 @22:49:45.69 Adding PP3 @22:49:45.70 delay 2 seconds @22:49:47.79 AS.seek 0.53ml @22:49:53.98 delay 10 seconds @22:50:04.00 ARV.dial :air2 @22:50:04.37 adding ARV air @22:50:04.38 delay 2 seconds @22:50:06.47 AS.seek 0.536ml @22:50:12.86 delay 5 seconds @22:50:17.88 ARV.dial :carrier @22:50:19.09 delay 7 seconds @22:50:26.18 AS.seek 0.594ml @22:51:25.01 delay 5 seconds @22:51:30.03 ATV.dial :ASV @22:51:30.42 delay 2 seconds @22:51:32.51 AS.seek 0.6ml @22:51:38.89 delay 4 seconds @22:51:42.91 ATV.dial ARV @22:51:43.42 delay 2 seconds @22:51:45.44 ARV.dial :carrier @22:51:45.46 delay 2 seconds @22:51:47.55 AS.seek 0.65ml @22:52:38.19 delay 4 seconds @22:52:42.21 ATV.dial PCR @22:52:42.78 ASV.dial :waste1 @22:52:42.79 ARV.dial :air1 @22:52:43.20 Positioning Slug by 0.324ml @22:52:43.29 AS.seek 0.326ml @22:58:09.55 delay 5 seconds @22:58:14.57 ATV.dial ARV @22:58:15.29 ASV.dial :PCRlock @22:58:15.69 delay 5 seconds @22:58:20.72 Running Rp Profile60C300 thermocycling @22:58:20.74 PCR.program Profile60C300 @22:58:20.87 Profile60C300 should complete in < 1:45:21 @22:58:20.88 PCR.run 2:16:41.25 @00:20:23.83CEST02-Oct-18 PCR.off @00:20:25.39 PCR for Rp completed -- closed /var/log/esp/Rp18oct0122h1425ml.pcr @00:20:25.46 Cleaning PCR @00:20:25.47 AFV.select :carrierSolution @00:20:25.58 delay 2 seconds @00:20:27.61 AS.reconfigure ASconfig @00:20:27.66 AS.seek 0.5ml @00:20:37.96 delay 1 second @00:20:38.97 AFV.select :holdingCoil @00:20:39.08 ASV.dial :waste1 @00:20:39.46 ATV.dial :air3 @00:20:40.75 AS.reconfigure ASslow1 @00:20:40.80 AS.seek 0.51ml @00:20:43.12 delay 2 seconds @00:20:45.14 ATV.dial :bleach @00:20:45.72 AS.seek 0.81ml @00:21:46.32 delay 3 seconds @00:21:49.34 ATV.dial :air3 @00:21:49.87 delay 3 seconds @00:21:52.96 AS.seek 0.835ml @00:21:58.26 delay 3 seconds @00:22:01.28 ATV.dial :bleach @00:22:01.87 AS.seek 0.82ml @00:22:05.15 delay 3 seconds @00:22:08.18 ARV.dial :waste @00:22:08.76 delay 3 seconds @00:22:11.78 ATV.dial :ARV @00:22:12.61 AS.reconfigure ASconfig @00:22:12.66 AS.seek 0.47ml @00:22:33.04 AFV.select :carrierSolution @00:22:33.16 delay 2 seconds @00:22:35.19 AS.seek 1ml @00:23:05.93 delay 1 second @00:23:06.95 AFV.select :holdingCoil @00:23:07.06 delay 1 second @00:23:08.09 AS.reconfigure ASfast @00:23:08.14 AS.seek 0ml @00:23:35.45 AFV.select :carrierSolution @00:23:35.57 delay 2 seconds @00:23:37.60 AS.reconfigure ASconfig @00:23:37.65 AS.seek 1ml @00:24:35.39 delay 1 second @00:24:36.41 AFV.select :holdingCoil @00:24:36.52 delay 1 second @00:24:37.55 AS.reconfigure ASfast @00:24:37.60 AS.seek 0ml @00:25:04.94 AFV.select :carrierSolution @00:25:05.06 delay 2 seconds @00:25:07.67 AS.reconfigure ASconfig @00:25:07.73 AS.seek 1ml @00:26:05.51 delay 1 second @00:26:06.52 AFV.select :holdingCoil @00:26:06.63 delay 1 second @00:26:07.66 AS.reconfigure ASfast @00:26:07.71 AS.seek 0ml @00:26:35.05 ARV.dial between carrier and air1 @00:26:35.54 ATV.dial :air3 @00:26:36.75 AS.reconfigure ASslow1 @00:26:36.80 AS.seek 0.01ml @00:26:39.15 delay 2 seconds @00:26:41.16 ATV.dial :bleach @00:26:41.77 AS.seek 0.81ml @00:29:22.92 delay 3 seconds @00:29:25.93 ATV.dial :air3 @00:29:26.46 delay 3 seconds @00:29:29.55 AS.seek 0.835ml @00:29:34.96 delay 3 seconds @00:29:37.98 ATV.dial :bleach @00:29:38.58 AS.seek 0.82ml @00:29:41.84 delay 3 seconds @00:29:44.86 ATV.dial PCR @00:29:45.24 AS.reconfigure ASconfig @00:29:45.29 AS.seek :empty @00:30:32.75 PCR.program :bleachProfile @00:30:32.81 PCR program should complete in < 2:45 @00:30:32.82 PCR.run 10:00 @00:33:40.71 PCR.off @00:33:40.73 AFV.select :carrierSolution @00:33:40.84 delay 2 seconds @00:33:42.87 AS.seek 1ml @00:34:40.73 delay 1 second @00:34:41.74 AFV.select :holdingCoil @00:34:41.85 delay 1 second @00:34:42.88 AS.reconfigure ASfast @00:34:42.93 AS.seek 0ml @00:35:10.29 delay 3 seconds @00:35:13.30 AFV.select :carrierSolution @00:35:13.41 delay 2 seconds @00:35:15.44 AS.reconfigure ASconfig @00:35:15.49 AS.seek 1ml @00:36:13.26 delay 1 second @00:36:14.27 AFV.select :holdingCoil @00:36:14.38 delay 1 second @00:36:15.41 AS.reconfigure ASfast @00:36:15.46 AS.seek 0ml @00:36:42.81 delay 3 seconds @00:36:45.82 AFV.select :carrierSolution @00:36:45.93 delay 2 seconds @00:36:47.96 AS.reconfigure ASconfig @00:36:48.01 AS.seek 1ml @00:37:45.84 delay 1 second @00:37:46.85 AFV.select :holdingCoil @00:37:46.96 delay 1 second @00:37:47.99 AS.reconfigure ASfast @00:37:48.04 AS.seek 0ml @00:38:15.40 delay 3 seconds @00:38:18.42 AFV.select :carrierSolution @00:38:18.53 delay 2 seconds @00:38:20.56 AS.reconfigure ASconfig @00:38:20.61 AS.seek 1ml @00:39:18.40 delay 1 second @00:39:19.41 AFV.select :holdingCoil @00:39:19.52 delay 1 second @00:39:20.55 AS.reconfigure ASfast @00:39:20.60 AS.seek 0ml @00:39:47.93 delay 3 seconds @00:39:50.95 AFV.select :carrierSolution @00:39:51.06 delay 2 seconds @00:39:53.09 AS.reconfigure ASconfig @00:39:53.14 AS.seek 1ml @00:40:50.92 delay 1 second @00:40:51.94 AFV.select :holdingCoil @00:40:52.05 delay 1 second @00:40:53.08 AS.reconfigure ASnorm @00:40:53.13 AS.seek 0ml @00:41:43.84 Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0200h1425ml.pcr @00:41:43.86 Building PCR Slug from ASV with MM3 mix, PP5 primer @00:41:43.87 Priming with carrier @00:41:43.88 ATV.dial ARV @00:41:44.56 ARV.dial :carrier @00:41:44.91 AS.seek 0.5ml @00:42:10.50 AFV.select :carrierSolution @00:42:10.62 delay 2 seconds @00:42:12.65 AS.reconfigure ASconfig @00:42:12.70 AS.seek 1ml @00:42:41.72 delay 1 second @00:42:42.73 AFV.select :holdingCoil @00:42:42.84 delay 1 second @00:42:43.86 ATV.dial :waste @00:42:45.37 AS.reconfigure ASnorm @00:42:45.42 AS.seek :empty @00:43:36.14 AFV.select :carrierSolution @00:43:36.26 delay 2 seconds @00:43:38.29 AS.reconfigure ASconfig @00:43:38.34 AS.seek 0.5ml @00:44:07.98 delay 1 second @00:44:08.99 AFV.select :holdingCoil @00:44:09.10 Building Slug @00:44:09.11 ATV.dial :ARV @00:44:10.61 delay 2 seconds @00:44:12.63 ARV.dial :air1 @00:44:13.13 delay 2 seconds @00:44:15.21 AS.reconfigure ASslow2 @00:44:15.27 AS.seek 0.506ml @00:44:21.43 delay 2 seconds @00:44:23.45 ARV.dial :MM3 @00:44:24.44 delay 2 seconds @00:44:26.47 Adding MM3 @00:44:26.55 AS.seek 0.524ml @00:44:44.84 delay 25 seconds @00:45:09.86 ARV.dial :PP5 @00:45:10.25 Adding PP5 @00:45:10.26 delay 2 seconds @00:45:12.35 AS.seek 0.53ml @00:45:18.51 delay 10 seconds @00:45:28.53 ARV.dial :air3 @00:45:28.90 adding ARV air @00:45:28.91 delay 2 seconds @00:45:31.00 AS.seek 0.536ml @00:45:37.33 delay 5 seconds @00:45:42.35 ARV.dial :carrier @00:45:43.54 delay 7 seconds @00:45:50.63 AS.seek 0.594ml @00:46:49.31 delay 5 seconds @00:46:54.33 ATV.dial :ASV @00:46:54.83 delay 2 seconds @00:46:56.92 AS.seek 0.6ml @00:47:03.09 delay 4 seconds @00:47:07.11 ATV.dial ARV @00:47:07.62 delay 2 seconds @00:47:09.64 ARV.dial :carrier @00:47:09.66 delay 2 seconds @00:47:11.75 AS.seek 0.65ml @00:48:02.26 delay 4 seconds @00:48:06.28 ATV.dial PCR @00:48:06.86 ASV.dial :waste1 @00:48:06.87 ARV.dial :air1 @00:48:07.27 Positioning Slug by 0.324ml @00:48:07.36 AS.seek 0.326ml @00:53:33.42 delay 5 seconds @00:53:38.44 ATV.dial ARV @00:53:39.27 ASV.dial :PCRlock @00:53:39.67 delay 5 seconds @00:53:44.70 Running Bb Profile60C300 thermocycling @00:53:44.71 PCR.program Profile60C300 @00:53:44.85 Profile60C300 should complete in < 1:45:21 @00:53:44.86 PCR.run 2:16:41.25 @02:15:24.53 PCR.off @02:15:26.09 PCR for Bb completed -- closed /var/log/esp/Bb18oct0200h1425ml.pcr @02:15:26.16 Cleaning PCR @02:15:26.17 AFV.select :carrierSolution @02:15:26.28 delay 2 seconds @02:15:28.31 AS.reconfigure ASconfig @02:15:28.36 AS.seek 0.5ml @02:15:38.65 delay 1 second @02:15:39.67 AFV.select :holdingCoil @02:15:39.78 ASV.dial :waste1 @02:15:40.15 ATV.dial :air3 @02:15:41.33 AS.reconfigure ASslow1 @02:15:41.38 AS.seek 0.51ml @02:15:43.68 delay 2 seconds @02:15:45.70 ATV.dial :bleach @02:15:46.30 AS.seek 0.81ml @02:16:46.87 delay 3 seconds @02:16:49.89 ATV.dial :air3 @02:16:50.43 delay 3 seconds @02:16:53.51 AS.seek 0.835ml @02:16:58.81 delay 3 seconds @02:17:01.83 ATV.dial :bleach @02:17:02.44 AS.seek 0.82ml @02:17:05.72 delay 3 seconds @02:17:08.75 ARV.dial :waste @02:17:09.33 delay 3 seconds @02:17:12.35 ATV.dial :ARV @02:17:13.29 AS.reconfigure ASconfig @02:17:13.34 AS.seek 0.47ml @02:17:33.73 AFV.select :carrierSolution @02:17:33.85 delay 2 seconds @02:17:35.88 AS.seek 1ml @02:18:06.62 delay 1 second @02:18:07.64 AFV.select :holdingCoil @02:18:07.75 delay 1 second @02:18:08.78 AS.reconfigure ASfast @02:18:08.83 AS.seek 0ml @02:18:36.14 AFV.select :carrierSolution @02:18:36.26 delay 2 seconds @02:18:38.29 AS.reconfigure ASconfig @02:18:38.34 AS.seek 1ml @02:19:36.09 delay 1 second @02:19:37.10 AFV.select :holdingCoil @02:19:37.21 delay 1 second @02:19:38.24 AS.reconfigure ASfast @02:19:38.29 AS.seek 0ml @02:20:05.64 AFV.select :carrierSolution @02:20:05.76 delay 2 seconds @02:20:07.79 AS.reconfigure ASconfig @02:20:07.84 AS.seek 1ml @02:21:06.16 delay 1 second @02:21:07.17 AFV.select :holdingCoil @02:21:07.28 delay 1 second @02:21:08.31 AS.reconfigure ASfast @02:21:08.36 AS.seek 0ml @02:21:35.69 ARV.dial between carrier and air1 @02:21:36.18 ATV.dial :air3 @02:21:37.37 AS.reconfigure ASslow1 @02:21:37.42 AS.seek 0.01ml @02:21:39.80 delay 2 seconds @02:21:41.81 ATV.dial :bleach @02:21:42.41 AS.seek 0.81ml @02:24:23.63 delay 3 seconds @02:24:26.65 ATV.dial :air3 @02:24:27.18 delay 3 seconds @02:24:30.27 AS.seek 0.835ml @02:24:35.57 delay 3 seconds @02:24:38.59 ATV.dial :bleach @02:24:39.19 AS.seek 0.82ml @02:24:42.49 delay 3 seconds @02:24:45.50 ATV.dial PCR @02:24:45.88 AS.reconfigure ASconfig @02:24:45.93 AS.seek :empty @02:25:33.41 PCR.program :bleachProfile @02:25:33.48 PCR program should complete in < 2:45 @02:25:33.49 PCR.run 10:00 @02:28:41.37 PCR.off @02:28:41.39 AFV.select :carrierSolution @02:28:41.51 delay 2 seconds @02:28:43.54 AS.seek 1ml @02:29:41.39 delay 1 second @02:29:42.40 AFV.select :holdingCoil @02:29:42.51 delay 1 second @02:29:43.54 AS.reconfigure ASfast @02:29:43.59 AS.seek 0ml @02:30:10.93 delay 3 seconds @02:30:13.95 AFV.select :carrierSolution @02:30:14.06 delay 2 seconds @02:30:16.09 AS.reconfigure ASconfig @02:30:16.14 AS.seek 1ml @02:31:13.92 delay 1 second @02:31:14.93 AFV.select :holdingCoil @02:31:15.04 delay 1 second @02:31:16.07 AS.reconfigure ASfast @02:31:16.12 AS.seek 0ml @02:31:43.57 delay 3 seconds @02:31:46.59 AFV.select :carrierSolution @02:31:46.70 delay 2 seconds @02:31:48.73 AS.reconfigure ASconfig @02:31:48.78 AS.seek 1ml @02:32:46.56 delay 1 second @02:32:47.57 AFV.select :holdingCoil @02:32:47.68 delay 1 second @02:32:48.71 AS.reconfigure ASfast @02:32:48.76 AS.seek 0ml @02:33:16.10 delay 3 seconds @02:33:19.12 AFV.select :carrierSolution @02:33:19.23 delay 2 seconds @02:33:21.26 AS.reconfigure ASconfig @02:33:21.32 AS.seek 1ml @02:34:19.11 delay 1 second @02:34:20.12 AFV.select :holdingCoil @02:34:20.23 delay 1 second @02:34:21.26 AS.reconfigure ASfast @02:34:21.31 AS.seek 0ml @02:34:48.65 delay 3 seconds @02:34:51.67 AFV.select :carrierSolution @02:34:51.78 delay 2 seconds @02:34:53.81 AS.reconfigure ASconfig @02:34:53.86 AS.seek 1ml @02:35:51.65 delay 1 second @02:35:52.66 AFV.select :holdingCoil @02:35:52.77 delay 1 second @02:35:53.80 AS.reconfigure ASnorm @02:35:53.85 AS.seek 0ml @02:36:44.54 Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0202h1425ml.pcr @02:36:44.56 Building PCR Slug from ASV with MM1 mix, PP2 primer @02:36:44.58 Priming with carrier ATV.dial ARV @02:36:45.24 ARV.dial :carrier @02:36:45.60 AS.seek 0.5ml @02:37:11.19 AFV.select :carrierSolution @02:37:11.31 delay 2 seconds @02:37:13.34 AS.reconfigure ASconfig @02:37:13.39 AS.seek 1ml @02:37:42.43 delay 1 second @02:37:43.44 AFV.select :holdingCoil @02:37:43.55 delay 1 second @02:37:44.58 ATV.dial :waste @02:37:46.09 AS.reconfigure ASnorm @02:37:46.14 AS.seek :empty @02:38:36.86 AFV.select :carrierSolution @02:38:36.98 delay 2 seconds @02:38:39.01 AS.reconfigure ASconfig @02:38:39.06 AS.seek 0.5ml @02:39:08.16 delay 1 second @02:39:09.18 AFV.select :holdingCoil @02:39:09.29 Building Slug @02:39:09.90 ATV.dial :ARV @02:39:11.40 delay 2 seconds @02:39:13.42 ARV.dial :air1 @02:39:13.83 delay 2 seconds @02:39:15.92 AS.reconfigure ASslow2 @02:39:15.97 AS.seek 0.506ml @02:39:22.18 delay 2 seconds @02:39:24.20 ARV.dial :MM1 @02:39:24.78 delay 2 seconds @02:39:26.81 Adding MM1 @02:39:26.88 AS.seek 0.524ml @02:39:45.26 delay 25 seconds @02:40:10.28 ARV.dial :PP2 @02:40:10.67 Adding PP2 @02:40:10.68 delay 2 seconds @02:40:12.77 AS.seek 0.53ml @02:40:19.33 delay 10 seconds @02:40:29.35 ARV.dial :air2 @02:40:29.73 adding ARV air @02:40:29.74 delay 2 seconds @02:40:31.83 AS.seek 0.536ml @02:40:38.03 delay 5 seconds @02:40:43.05 ARV.dial :carrier @02:40:44.26 delay 7 seconds @02:40:51.35 AS.seek 0.594ml @02:41:50.12 delay 5 seconds @02:41:55.14 ATV.dial :ASV @02:41:55.54 delay 2 seconds @02:41:57.63 AS.seek 0.6ml @02:42:03.89 delay 4 seconds @02:42:07.91 ATV.dial ARV @02:42:08.53 delay 2 seconds @02:42:10.55 ARV.dial :carrier @02:42:10.57 delay 2 seconds @02:42:12.66 AS.seek 0.65ml @02:43:03.12 delay 4 seconds @02:43:07.14 ATV.dial PCR @02:43:07.82 ASV.dial :waste1 @02:43:07.84 ARV.dial :air1 @02:43:08.24 Positioning Slug by 0.324ml @02:43:08.33 AS.seek 0.326ml @02:48:34.61 delay 5 seconds @02:48:39.63 ATV.dial ARV @02:48:40.44 ASV.dial :PCRlock @02:48:40.83 delay 5 seconds @02:48:45.86 Running Ss Profile60C350 thermocycling @02:48:45.87 PCR.program Profile60C350 @02:48:46.01 Profile60C350 should complete in < 1:48:05 @02:48:46.02 PCR.run 2:20:06.25 @04:11:14.09 PCR.off @04:11:16.13 PCR for Ss completed -- closed /var/log/esp/Ss18oct0202h1425ml.pcr @04:11:16.20 Cleaning PCR @04:11:16.21 AFV.select :carrierSolution @04:11:16.32 delay 2 seconds @04:11:18.35 AS.reconfigure ASconfig @04:11:18.40 AS.seek 0.5ml @04:11:28.71 delay 1 second @04:11:29.73 AFV.select :holdingCoil @04:11:29.84 ASV.dial :waste1 @04:11:30.23 ATV.dial :air3 @04:11:31.43 AS.reconfigure ASslow1 @04:11:31.48 AS.seek 0.51ml @04:11:33.79 delay 2 seconds @04:11:35.81 ATV.dial :bleach @04:11:36.40 AS.seek 0.81ml @04:12:37.06 delay 3 seconds @04:12:40.08 ATV.dial :air3 @04:12:40.62 delay 3 seconds @04:12:43.70 AS.seek 0.835ml @04:12:49.08 delay 3 seconds @04:12:52.09 ATV.dial :bleach @04:12:52.69 AS.seek 0.82ml @04:12:55.96 delay 3 seconds @04:12:58.98 ARV.dial :waste @04:12:59.57 delay 3 seconds @04:13:02.59 ATV.dial :ARV @04:13:03.43 AS.reconfigure ASconfig @04:13:03.48 AS.seek 0.47ml @04:13:23.89 AFV.select :carrierSolution @04:13:24.01 delay 2 seconds @04:13:26.04 AS.seek 1ml @04:13:56.78 delay 1 second @04:13:58.35 AFV.select :holdingCoil @04:13:58.48 delay 1 second @04:13:59.51 AS.reconfigure ASfast @04:13:59.56 AS.seek 0ml @04:14:26.89 AFV.select :carrierSolution @04:14:27.01 delay 2 seconds @04:14:29.04 AS.reconfigure ASconfig @04:14:29.09 AS.seek 1ml @04:15:26.88 delay 1 second @04:15:27.89 AFV.select :holdingCoil @04:15:28.00 delay 1 second @04:15:29.03 AS.reconfigure ASfast @04:15:29.08 AS.seek 0ml @04:15:56.42 AFV.select :carrierSolution @04:15:56.54 delay 2 seconds @04:15:58.57 AS.reconfigure ASconfig @04:15:58.62 AS.seek 1ml @04:16:56.45 delay 1 second @04:16:57.46 AFV.select :holdingCoil @04:16:57.57 delay 1 second @04:16:58.60 AS.reconfigure ASfast @04:16:58.65 AS.seek 0ml @04:17:26.00 ARV.dial between carrier and air1 @04:17:26.49 ATV.dial :air3 @04:17:27.70 AS.reconfigure ASslow1 @04:17:27.75 AS.seek 0.01ml @04:17:30.09 delay 2 seconds @04:17:32.10 ATV.dial :bleach @04:17:32.70 AS.seek 0.81ml @04:20:13.84 delay 3 seconds @04:20:16.86 ATV.dial :air3 @04:20:17.38 delay 3 seconds @04:20:20.47 AS.seek 0.835ml @04:20:25.74 delay 3 seconds @04:20:28.76 ATV.dial :bleach @04:20:29.36 AS.seek 0.82ml @04:20:32.66 delay 3 seconds @04:20:35.67 ATV.dial PCR @04:20:36.06 AS.reconfigure ASconfig @04:20:36.11 AS.seek :empty @04:21:23.58 PCR.program :bleachProfile @04:21:23.64 PCR program should complete in < 2:45 @04:21:23.65 PCR.run 10:00 @04:24:31.53 PCR.off @04:24:31.55 AFV.select :carrierSolution @04:24:31.66 delay 2 seconds @04:24:33.69 AS.seek 1ml @04:25:31.53 delay 1 second @04:25:32.55 AFV.select :holdingCoil @04:25:32.66 delay 1 second @04:25:33.69 AS.reconfigure ASfast @04:25:33.74 AS.seek 0ml @04:26:01.10 delay 3 seconds @04:26:04.12 AFV.select :carrierSolution @04:26:04.23 delay 2 seconds @04:26:06.26 AS.reconfigure ASconfig @04:26:06.32 AS.seek 1ml @04:27:04.11 delay 1 second @04:27:05.13 AFV.select :holdingCoil @04:27:05.24 delay 1 second @04:27:06.27 AS.reconfigure ASfast @04:27:06.32 AS.seek 0ml @04:27:33.67 delay 3 seconds @04:27:36.69 AFV.select :carrierSolution @04:27:36.80 delay 2 seconds @04:27:38.83 AS.reconfigure ASconfig @04:27:38.88 AS.seek 1ml @04:28:36.64 delay 1 second @04:28:37.66 AFV.select :holdingCoil @04:28:37.77 delay 1 second @04:28:38.80 AS.reconfigure ASfast @04:28:38.85 AS.seek 0ml @04:29:06.18 delay 3 seconds @04:29:09.20 AFV.select :carrierSolution @04:29:09.31 delay 2 seconds @04:29:11.34 AS.reconfigure ASconfig @04:29:11.40 AS.seek 1ml @04:30:09.20 delay 1 second @04:30:10.21 AFV.select :holdingCoil @04:30:10.32 delay 1 second @04:30:11.35 AS.reconfigure ASfast @04:30:11.40 AS.seek 0ml @04:30:38.76 delay 3 seconds @04:30:41.78 AFV.select :carrierSolution @04:30:41.89 delay 2 seconds @04:30:43.92 AS.reconfigure ASconfig @04:30:43.97 AS.seek 1ml @04:31:41.74 delay 1 second @04:31:42.76 AFV.select :holdingCoil @04:31:42.87 delay 1 second @04:31:43.90 AS.reconfigure ASnorm @04:31:43.95 AS.seek 0ml @04:32:34.65 Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0204h1425ml.pcr @04:32:34.67 Building PCR Slug from ASV with MM1 mix, PP1 primer @04:32:34.69 Priming with carrier ATV.dial ARV @04:32:35.25 ARV.dial :carrier @04:32:35.60 AS.seek 0.5ml @04:33:01.19 AFV.select :carrierSolution @04:33:01.31 delay 2 seconds @04:33:03.92 AS.reconfigure ASconfig @04:33:03.98 AS.seek 1ml @04:33:33.00 delay 1 second @04:33:34.02 AFV.select :holdingCoil @04:33:34.13 delay 1 second @04:33:35.15 ATV.dial :waste @04:33:36.68 AS.reconfigure ASnorm @04:33:36.73 AS.seek :empty @04:34:27.47 AFV.select :carrierSolution @04:34:27.59 delay 2 seconds @04:34:29.62 AS.reconfigure ASconfig @04:34:29.67 AS.seek 0.5ml @04:34:58.75 delay 1 second @04:34:59.77 AFV.select :holdingCoil @04:34:59.88 Building Slug @04:34:59.89 ATV.dial :ARV @04:35:01.39 delay 2 seconds @04:35:03.41 ARV.dial :air1 @04:35:03.80 delay 2 seconds @04:35:05.89 AS.reconfigure ASslow2 @04:35:05.94 AS.seek 0.506ml @04:35:12.25 delay 2 seconds @04:35:14.26 ARV.dial :MM1 @04:35:14.84 delay 2 seconds @04:35:16.87 Adding MM1 @04:35:17.00 AS.seek 0.524ml @04:35:35.37 delay 25 seconds @04:36:00.39 ARV.dial :PP1 @04:36:00.78 Adding PP1 @04:36:00.80 delay 2 seconds @04:36:02.88 AS.seek 0.53ml @04:36:09.34 delay 10 seconds @04:36:19.36 ARV.dial :air1 @04:36:19.73 adding ARV air @04:36:19.74 delay 2 seconds @04:36:21.83 AS.seek 0.536ml @04:36:27.98 delay 5 seconds @04:36:33.00 ARV.dial :carrier @04:36:33.39 delay 7 seconds @04:36:40.48 AS.seek 0.594ml @04:37:39.20 delay 5 seconds @04:37:44.22 ATV.dial :ASV @04:37:44.62 delay 2 seconds @04:37:46.71 AS.seek 0.6ml @04:37:52.97 delay 4 seconds @04:37:56.99 ATV.dial ARV @04:37:57.62 delay 2 seconds @04:37:59.64 ARV.dial :carrier @04:37:59.66 delay 2 seconds @04:38:01.75 AS.seek 0.65ml @04:38:52.39 delay 4 seconds @04:38:56.41 ATV.dial PCR @04:38:56.97 ASV.dial :waste1 @04:38:56.99 ARV.dial :air1 @04:38:57.37 Positioning Slug by 0.324ml @04:38:57.46 AS.seek 0.326ml @04:44:23.66 delay 5 seconds @04:44:28.68 ATV.dial ARV @04:44:29.41 ASV.dial :PCRlock @04:44:29.80 delay 5 seconds @04:44:34.83 Running IPc ProfileIPc thermocycling @04:44:34.84 PCR.program ProfileIPc @04:44:35.00 ProfileIPc should complete in < 1:50:49 @04:44:35.01 PCR.run 2:23:31.25 @06:08:51.38 PCR.off @06:08:54.53 PCR for IPc completed -- closed /var/log/esp/IPc18oct0204h1425ml.pcr @06:08:54.55 Full MFB Decontamination @06:08:54.56 Decon extraction column and decon AIV @06:08:54.58 SPE.hold 2:46:40,55C @06:08:54.63 ASV.dial :SPEdelivery @06:08:55.23 delay 20 seconds @06:09:15.25 AFV.select :carrierSolution @06:09:15.36 delay 2 seconds @06:09:17.39 AS.reconfigure ASconfig @06:09:17.44 AS.seek 0.5ml @06:09:27.79 delay 1 second @06:09:28.80 AFV.select :holdingCoil @06:09:28.92 ATV.dial :air1 @06:09:30.55 AS.reconfigure ASslow1 @06:09:30.60 AS.seek 0.52ml @06:09:34.89 ATV.dial :ASV @06:09:36.40 ASV.dial :SPErecovery @06:09:37.01 AS.seek 0.62ml @06:09:57.37 ATV.dial :waste @06:09:59.07 AS.reconfigure ASnorm @06:09:59.12 AS.seek :empty @06:10:30.75 AFV.select :holdingCoil @06:10:30.77 ATV.dial :waste @06:10:30.79 AS.reconfigure ASconfig @06:10:30.84 AS.seek 0ml @06:10:31.14 AFV.select :carrierSolution @06:10:31.26 delay 2 seconds @06:10:33.29 AS.seek 0.5ml @06:11:02.30 delay 1 second @06:11:03.31 AFV.select :holdingCoil @06:11:03.42 ATV.dial :air3 @06:11:04.06 AS.reconfigure ASslow1 @06:11:04.11 AS.seek 0.52ml @06:11:08.37 ATV.dial :air3 @06:11:08.46 AS.seek 0.53ml @06:11:10.82 delay 2 seconds @06:11:12.83 ATV.dial :bleach @06:11:13.30 AS.seek 0.71ml @06:11:49.87 delay 3 seconds @06:11:52.89 ATV.dial :air3 @06:11:53.41 delay 3 seconds @06:11:56.49 AS.seek 0.735ml @06:12:01.92 delay 3 seconds @06:12:04.94 ATV.dial :bleach @06:12:05.55 AS.seek 0.72ml @06:12:08.90 delay 3 seconds @06:12:11.92 ATV.dial ASV @06:12:12.97 ASV.dial :SPErecovery @06:12:13.06 AS.seek 0.365ml @06:13:24.75 ATV.dial :waste @06:13:26.32 AS.reconfigure ASnorm @06:13:26.37 AS.seek :empty @06:13:45.15 ATV.dial :air3 @06:13:45.80 AS.reconfigure ASslow1 @06:13:45.85 AS.seek 0.009ml @06:13:48.13 delay 2 seconds @06:13:50.14 ATV.dial :bleach @06:13:50.61 AS.seek 0.579ml @06:15:45.56 delay 3 seconds @06:15:48.59 ATV.dial :air3 @06:15:49.12 delay 3 seconds @06:15:52.21 AS.seek 0.604ml @06:15:57.52 delay 3 seconds @06:16:00.54 ATV.dial :bleach @06:16:01.14 AS.seek 0.589ml @06:16:04.45 delay 3 seconds @06:16:07.47 ATV.dial :ASV @06:16:08.40 ASV.dial :wasteR @06:16:08.78 AS.seek 0ml @06:18:07.56 delay 1:00 @06:19:07.58 ATV.dial ASV @06:19:07.60 AFV.select :carrierSolution @06:19:07.70 delay 2 seconds @06:19:09.73 AS.reconfigure ASconfig @06:19:09.77 AS.seek 1ml @06:20:07.54 delay 1 second @06:20:08.55 AFV.select :holdingCoil @06:20:08.66 delay 1 second @06:20:09.68 ASV.dial :wasteR @06:20:09.70 AS.reconfigure ASslow1 @06:20:09.75 AS.seek 0ml @06:23:31.12 delay 10 seconds @06:23:41.14 ATV.dial :air3 @06:23:42.53 AS.seek 0.01ml @06:23:44.84 delay 2 seconds @06:23:46.85 ATV.dial :bleach @06:23:48.10 AS.seek 0.58ml @06:25:43.06 delay 3 seconds @06:25:46.08 ATV.dial :air3 @06:25:46.60 delay 3 seconds @06:25:49.69 AS.seek 0.605ml @06:25:54.98 delay 3 seconds @06:25:58.00 ATV.dial :bleach @06:25:58.60 AS.seek 0.59ml @06:26:01.93 delay 3 seconds @06:26:04.96 ATV.dial ASV @06:26:06.00 ASV.dial :SPEdelivery @06:26:06.38 AS.seek 0ml @06:28:05.31 delay 1:00 @06:29:05.33 AFV.select :carrierSolution @06:29:05.44 delay 2 seconds @06:29:07.47 AS.reconfigure ASconfig @06:29:07.52 AS.seek 1ml @06:30:05.28 delay 1 second @06:30:06.29 AFV.select :holdingCoil @06:30:06.40 delay 1 second @06:30:07.42 ATV.dial ASV @06:30:07.44 ASV.dial :SPEdelivery @06:30:07.46 AS.reconfigure ASslow1 @06:30:07.50 AS.seek 0ml @06:33:28.89 delay 10 seconds @06:33:38.91 ATV.dial :air2 @06:33:39.68 AS.reconfigure ASslow0 @06:33:39.74 AS.seek 0.8ml @06:35:00.51 delay 3 seconds @06:35:03.53 ATV.dial :ASV @06:35:04.44 ASV.dial :SPEdelivery @06:35:04.47 AS.reconfigure ASslow1 @06:35:04.52 AS.seek 0.03ml @06:37:39.62 delay 20 seconds @06:37:59.65 ATV.dial :waste,via: :air3 @06:38:01.22 AS.reconfigure ASconfig @06:38:01.27 AS.seek :empty @06:38:03.33 SPE.coast @06:38:03.42 Removing Bleach from Mixing Coil @06:38:03.43 AFV.select :carrierSolution @06:38:03.54 delay 2 seconds @06:38:05.57 AS.seek 0.5ml @06:38:34.69 delay 1 second @06:38:35.70 AFV.select :holdingCoil @06:38:35.81 ATV.dial :ASV @06:38:37.36 ASV.dial :SPErecovery @06:38:37.98 AS.reconfigure ASslow1 @06:38:38.04 AS.seek 0.73ml @06:39:24.57 ASV.dial :wasteD @06:39:25.12 AS.reconfigure ASnorm @06:39:25.17 AS.seek 0ml @06:40:02.30 AFV.select :carrierSolution @06:40:02.42 delay 2 seconds @06:40:04.45 AS.reconfigure ASconfig @06:40:04.50 AS.seek 0.5ml @06:40:33.53 delay 1 second @06:40:34.54 AFV.select :holdingCoil @06:40:34.65 ATV.dial ASV @06:40:34.66 ASV.dial :SPErecovery @06:40:35.28 AS.reconfigure ASslow0 @06:40:35.34 AS.seek 0.3ml @06:40:55.79 ATV.dial :air2 @06:40:56.76 AS.reconfigure ASslow1 @06:40:56.82 AS.seek 0.32ml @06:41:01.16 ATV.dial ASV @06:41:02.12 AS.reconfigure ASslow0 @06:41:02.18 AS.seek 0.57ml @06:41:27.68 ASV.dial :wasteD @06:41:28.25 AS.reconfigure ASnorm @06:41:28.30 AS.seek 0ml @06:41:57.36 AFV.select :carrierSolution @06:41:57.48 delay 2 seconds @06:41:59.51 AS.reconfigure ASconfig @06:41:59.56 AS.seek 0.5ml @06:42:28.61 delay 1 second @06:42:29.63 AFV.select :holdingCoil @06:42:29.74 ATV.dial ASV @06:42:29.75 ASV.dial :SPErecovery @06:42:30.37 AS.reconfigure ASslow0 @06:42:30.42 AS.seek 0.3ml @06:42:50.86 ATV.dial :air2 @06:42:51.93 AS.reconfigure ASslow1 @06:42:51.99 AS.seek 0.32ml @06:42:56.30 ATV.dial ASV @06:42:57.26 AS.reconfigure ASslow0 @06:42:57.32 AS.seek 0.57ml @06:43:22.77 ASV.dial :wasteD @06:43:23.33 AS.reconfigure ASnorm @06:43:23.38 AS.seek 0ml @06:43:52.44 ATV.dial :air2 @06:43:54.06 AS.reconfigure ASslow0 @06:43:54.11 AS.seek 0.3ml @06:44:24.63 ATV.dial :ASV @06:44:25.64 ASV.dial :wasteD @06:44:25.66 AS.reconfigure ASconfig @06:44:25.71 AS.seek 0.05ml @06:44:40.38 ATV.dial :waste @06:44:42.08 AS.seek 0ml @06:44:45.22 ASV.dial :SPEdelivery @06:44:46.16 deconLysateINT @06:44:46.17 ATV.dial :air3 @06:44:46.82 AS.reconfigure ASslow1 @06:44:46.87 AS.seek 0.01ml @06:44:49.22 delay 2 seconds @06:44:51.23 ATV.dial :bleach @06:44:51.69 AS.seek 0.96ml @06:48:02.98 delay 3 seconds @06:48:06.00 ATV.dial :air3 @06:48:06.53 delay 3 seconds @06:48:09.62 AS.seek 0.985ml @06:48:14.90 delay 3 seconds @06:48:17.92 ATV.dial :bleach @06:48:18.52 AS.seek 0.97ml @06:48:21.83 delay 3 seconds @06:48:24.86 ATV.dial AIV @06:48:26.00 AIV.dial :coilToMFB @06:48:26.01 AWV.select :waste @06:48:26.02 AFV.select :carrierSolution @06:48:26.14 delay 2 seconds @06:48:28.17 AS.reconfigure ASconfig @06:48:28.22 AS.seek 1ml @06:48:30.28 delay 1 second @06:48:31.29 AFV.select :holdingCoil @06:48:31.40 delay 1 second @06:48:32.43 AS.reconfigure ASslow0 @06:48:32.47 AS.seek :empty @06:50:13.43 AFV.select :carrierSolution @06:50:13.55 delay 2 seconds @06:50:15.58 AS.reconfigure ASconfig @06:50:15.63 AS.seek 1ml @06:51:13.42 delay 1 second @06:51:14.43 AFV.select :holdingCoil @06:51:14.54 delay 1 second @06:51:15.57 AS.reconfigure ASslow0 @06:51:15.61 AS.seek :empty @06:52:56.58 AFV.select :carrierSolution @06:52:56.70 delay 2 seconds @06:52:58.73 AS.reconfigure ASconfig @06:52:58.78 AS.seek 1ml @06:53:56.59 delay 1 second @06:53:57.60 AFV.select :holdingCoil @06:53:57.71 delay 1 second @06:53:58.74 AS.reconfigure ASslow0 @06:53:58.78 AS.seek :empty @06:55:39.73 ATV.dial :air2 @06:55:40.38 AS.reconfigure ASslow1 @06:55:40.43 AS.seek 0.009ml @06:55:42.72 AS.reconfigure ASslow0 @06:55:42.77 AS.seek 1ml @06:57:22.71 ATV.dial AIV @06:57:23.53 AS.seek 0.02ml @06:59:02.40 ATV.dial :waste @06:59:03.91 AS.seek 0ml @06:59:06.21 AWV.select :air @06:59:06.38 Cleaning PCR @06:59:06.39 AFV.select :carrierSolution @06:59:06.50 delay 2 seconds @06:59:08.53 AS.reconfigure ASconfig @06:59:08.58 AS.seek 0.5ml @06:59:37.61 delay 1 second @06:59:38.62 AFV.select :holdingCoil @06:59:38.73 ASV.dial :waste1 @06:59:39.17 ATV.dial :air3 @06:59:39.80 AS.reconfigure ASslow1 @06:59:39.85 AS.seek 0.51ml @06:59:42.13 delay 2 seconds @06:59:44.14 ATV.dial :bleach @06:59:44.60 AS.seek 0.81ml @07:00:45.24 delay 3 seconds @07:00:48.26 ATV.dial :air3 @07:00:48.78 delay 3 seconds @07:00:51.87 AS.seek 0.835ml @07:00:57.16 delay 3 seconds @07:01:00.17 ATV.dial :bleach @07:01:00.78 AS.seek 0.82ml @07:01:04.14 delay 3 seconds @07:01:07.15 ARV.dial :waste @07:01:07.74 delay 3 seconds @07:01:10.76 ATV.dial :ARV @07:01:11.58 AS.reconfigure ASconfig @07:01:11.63 AS.seek 0.47ml @07:01:32.63 AFV.select :carrierSolution @07:01:32.75 delay 2 seconds @07:01:34.78 AS.seek 1ml @07:02:05.57 delay 1 second @07:02:06.58 AFV.select :holdingCoil @07:02:06.69 delay 1 second @07:02:07.72 AS.reconfigure ASfast @07:02:07.77 AS.seek 0ml @07:02:35.10 AFV.select :carrierSolution @07:02:35.22 delay 2 seconds @07:02:37.25 AS.reconfigure ASconfig @07:02:37.30 AS.seek 1ml @07:03:35.14 delay 1 second @07:03:36.15 AFV.select :holdingCoil @07:03:36.26 delay 1 second @07:03:37.29 AS.reconfigure ASfast @07:03:37.34 AS.seek 0ml @07:04:04.67 AFV.select :carrierSolution @07:04:04.79 delay 2 seconds @07:04:06.82 AS.reconfigure ASconfig @07:04:06.87 AS.seek 1ml @07:05:04.72 delay 1 second @07:05:05.73 AFV.select :holdingCoil @07:05:05.84 delay 1 second @07:05:06.87 AS.reconfigure ASfast @07:05:06.92 AS.seek 0ml @07:05:34.25 ARV.dial between carrier and air1 @07:05:34.74 ATV.dial :air3 @07:05:35.93 AS.reconfigure ASslow1 @07:05:35.98 AS.seek 0.01ml @07:05:38.33 delay 2 seconds @07:05:40.34 ATV.dial :bleach @07:05:40.93 AS.seek 0.81ml @07:08:22.07 delay 3 seconds @07:08:25.09 ATV.dial :air3 @07:08:25.61 delay 3 seconds @07:08:28.70 AS.seek 0.835ml @07:08:33.92 delay 3 seconds @07:08:36.94 ATV.dial :bleach @07:08:37.54 AS.seek 0.82ml @07:08:40.82 delay 3 seconds @07:08:43.84 ATV.dial PCR @07:08:44.22 AS.reconfigure ASconfig @07:08:44.27 AS.seek :empty @07:09:31.73 PCR.program :bleachProfile @07:09:31.80 PCR program should complete in < 2:45 @07:09:31.81 PCR.run 10:00 @07:12:51.81 PCR.off @07:12:51.83 AFV.select :carrierSolution @07:12:51.94 delay 2 seconds @07:12:53.97 AS.seek 1ml @07:13:51.80 delay 1 second @07:13:52.81 AFV.select :holdingCoil @07:13:52.92 delay 1 second @07:13:53.95 AS.reconfigure ASfast @07:13:54.00 AS.seek 0ml @07:14:21.33 delay 3 seconds @07:14:24.35 AFV.select :carrierSolution @07:14:24.46 delay 2 seconds @07:14:26.49 AS.reconfigure ASconfig @07:14:26.55 AS.seek 1ml @07:15:24.36 delay 1 second @07:15:25.38 AFV.select :holdingCoil @07:15:25.49 delay 1 second @07:15:26.52 AS.reconfigure ASfast @07:15:26.57 AS.seek 0ml @07:15:53.93 delay 3 seconds @07:15:56.95 AFV.select :carrierSolution @07:15:57.06 delay 2 seconds @07:15:59.09 AS.reconfigure ASconfig @07:15:59.14 AS.seek 1ml @07:16:56.91 delay 1 second @07:16:57.92 AFV.select :holdingCoil @07:16:58.03 delay 1 second @07:16:59.06 AS.reconfigure ASfast @07:16:59.11 AS.seek 0ml @07:17:26.43 delay 3 seconds @07:17:29.45 AFV.select :carrierSolution @07:17:29.56 delay 2 seconds @07:17:31.59 AS.reconfigure ASconfig @07:17:31.65 AS.seek 1ml @07:18:29.41 delay 1 second @07:18:30.42 AFV.select :holdingCoil @07:18:30.53 delay 1 second @07:18:31.56 AS.reconfigure ASfast @07:18:31.61 AS.seek 0ml @07:18:58.96 delay 3 seconds @07:19:01.98 AFV.select :carrierSolution @07:19:02.09 delay 2 seconds @07:19:04.12 AS.reconfigure ASconfig @07:19:04.17 AS.seek 1ml @07:20:01.96 delay 1 second @07:20:02.97 AFV.select :holdingCoil @07:20:03.08 delay 1 second @07:20:04.11 AS.reconfigure ASnorm @07:20:04.16 AS.seek 0ml @07:20:54.89 AFV.select :carrierSolution @07:20:55.00 delay 2 seconds @07:20:57.03 AS.reconfigure ASconfig @07:20:57.08 AS.seek 1ml @07:21:55.35 delay 1 second @07:21:56.44 AFV.select :holdingCoil @07:21:56.55 delay 1 second @07:21:57.57 ATV.dial :waste @07:21:58.70 AS.reconfigure ASnorm @07:21:58.76 AS.seek 0.5ml @07:22:24.34 ATV.dial :waste2 @07:22:25.51 AS.seek 0ml @07:22:51.04 AS.coast @07:22:51.08 SS.coast @07:22:51.12 CC.coast @07:22:51.16 CS.coast @07:22:51.20 PC.coast @07:22:51.24 PS.coast @07:22:51.28 Forearm.coast @07:22:51.32 Elbow.coast @07:22:51.72 Gate.power :core,:OFF @07:22:51.73 Gate.power :raw,:OFF @07:22:51.74 Shutdown @07:22:51.76 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @14:00:00.05
Powering core bus @14:00:05.16 Gate.power :raw,:ON @14:00:05.81 SPE.coast @14:00:05.87 SPE.configure SPEconfig @14:00:05.96 AS.configure ASconfig @14:00:06.06 SS.configure SSconfig @14:00:06.16 CC.configure CCconfig @14:00:06.26 CS.configure CSconfig @14:00:06.36 PC.configure PCconfig @14:00:06.46 PS.configure PSconfig @14:00:06.58 Forearm.configure LooseForearm @14:00:06.68 Elbow.configure LooseElbow @14:00:06.80 SC.configure SCloose @14:00:06.90 SE.configure SEconfig @14:00:06.95 CH.coast @14:00:07.00 CH.configure CHconfig @14:00:07.04 PH.coast @14:00:07.10 PH.configure PHconfig @14:00:07.14 PCR.off @14:00:07.17 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:07.20 PCR.caltemp= 2.7073,0.0373,0 @14:00:07.23 All dwarf controllers running firmware version 3.75 @14:00:07.35 Valve.reset @14:00:07.52 PCR.off @14:00:07.55 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:07.58 PCR.caltemp= 2.7073,0.0373,0 @14:00:07.82 PSV.dial :air @14:00:07.84 Intake.select :close @14:00:07.86 Exhaust.select :close @14:00:07.89 CTV.dial :air @14:00:08.00 CSV.dial :waste1 @14:00:08.14 SSV.dial :spit,avoiding: :exhaust @14:00:08.16
Forearm.reconfigure LooseForearm @14:00:08.28 Forearm.home.seek :retract @14:00:08.39 PS.reconfigure PSconfig @14:00:08.44 PS.home.seek 0ml @14:00:09.08 CS.reconfigure CSfast @14:00:09.10 SS.reconfigure SSconfig @14:00:09.20 CS.home.seek 0ml @14:00:09.23 SS.home.seek 0ml @14:00:09.87 Hand.close @14:00:09.91
Elbow.jog 1500 @14:00:10.52 Elbow.reconfigure LooseElbow @14:00:10.58 Elbow.home.seek :clear @14:00:13.94 SE.reconfigure SEconfig @14:00:13.98 SE.home.seek :down @14:00:14.62 SC.reconfigure SCsloppy @14:00:14.67 SC.home.seek :initial @14:00:16.87 Forearm.seek :retract @14:00:16.95 Elbow.seek :hammer @14:00:17.38 PS.coast @14:00:18.09
Forearm.reconfigure TightForearm @14:00:18.14 Forearm.seek 5 + 350 counts @14:00:19.19 Forearm.seek :retract @14:00:20.38 PC.reconfigure PCconfig @14:00:20.40
CC.reconfigure CCconfig @14:00:20.51 CC.home.seek :open @14:00:20.53 PC.home.seek :open @14:00:23.92
Phase [coreNEG] @14:00:23.93 GoodNews.email "At 14:00:23 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tundeployv2.rb:110\n{1 => 6, 2 => 16}\nCan@14:00:23, 14.6C, 67% humidity, 14.3psia, 14.699V, 0.682A, 0.676A avg, 10.03W",Subject: "Started 18tundeployv2 phase coreNEG" @14:00:23.96CEST02-Oct-18
==================> STARTED coreNEG (phase #2) <=== @14:00:23.98 Starting initialPurge @14:01:04.15 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:01:04.17 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:01:04.20 Retry #1 of 10 after 10:00 @14:01:04.30
Hand.open @14:01:05.08 Elbow.reconfigure TightElbow @14:01:05.12 Elbow.seek FlushPuck::Garage @14:01:07.02 Elbow.reconfigure LooseElbow @14:01:07.13 Forearm.reconfigure LooseForearm @14:01:07.18 Forearm.seek :garage @14:01:08.17 Hand.close @14:01:08.95 Forearm.seek :retract @14:01:09.97 Elbow.reconfigure TightElbow @14:01:10.02 Elbow.seek CC @14:01:12.95 Elbow.reconfigure LooseElbow @14:01:13.06 Forearm.reconfigure TightForearm @14:01:13.11 Forearm.seek CC @14:01:14.47 CC.seek :closed @14:01:18.72 Collection Clamp onSpring/offSpring for 6/32 samples, on/off torque 1.67 @14:01:18.81 Hand.open @14:01:19.51 Forearm.reconfigure LooseForearm @14:01:19.56 Forearm.seek :retract @14:01:20.55 Flushing the Collection Valve::Manifold @14:01:20.68 CSV.dial CSR @14:01:21.98 Pulling 5ml of CSR.flush @14:01:21.99 CSR.select :flush @14:01:22.11 CS.reconfigure CSconfig @14:01:22.15 CS.seek 5ml @14:01:35.87 delay 5 seconds @14:01:40.89 CSR.select :air @14:01:41.06 CS.reconfigure CSfast @14:01:41.11 CS.seek 7.5ml @14:01:47.52 CSV.dial :waste @14:01:48.76 CS.reconfigure CSconfig @14:01:48.81 CS.seek 0ml @14:02:08.93 Purging external Intake @14:02:09.06 CSV.dial :flush @14:02:09.51 CS.reconfigure CSfast @14:02:09.56 CS.seek 10ml @14:02:31.72 delay 5 seconds @14:02:36.74 CSV.dial CBV @14:02:36.77
CBV.dial CSV @14:02:37.51 CTV.dial :intake @14:02:38.24 Intake.select :open @14:02:38.37 CS.seek 0ml @14:03:01.30 Intake.select :close @14:03:01.42 CTV.dial :air @14:03:02.15 Flushing upper manifold @14:03:02.28 CSV.dial :flush @14:03:03.00 CS.seek 5ml @14:03:14.65 delay 5 seconds @14:03:19.67 CSV.dial :air @14:03:20.21 CS.seek 7ml @14:03:25.56 CSV.dial CBV @14:03:25.58
CBV.dial CSV @14:03:26.70 CTV.dial :air @14:03:26.72 CS.seek 0ml @14:03:42.50 delay 7 seconds @14:03:49.53 CS.seek 3ml @14:03:56.93 CSV.dial :waste @14:03:57.37 CS.seek 0ml @14:04:04.90 CSV.dial :flush @14:04:05.33 CS.seek 3ml @14:04:12.75 delay 5 seconds @14:04:17.77 CSV.dial :air @14:04:18.83 CS.seek 5ml @14:04:24.21 CSV.dial :air @14:04:24.24 CS.seek 0ml @14:04:35.72 delay 7 seconds @14:04:42.75 CS.seek 3ml @14:04:50.24 CSV.dial :waste @14:04:50.97 CS.seek 0ml @14:04:58.34 Garaging FlushPuck in Collection Clamp @14:04:58.42 Elbow.reconfigure TightElbow @14:04:58.47 Elbow.seek CC @14:04:58.73 Elbow.reconfigure LooseElbow @14:04:58.84 Forearm.seek CC @14:04:59.80 Hand.close @14:05:00.45 CC.seek :open @14:05:04.79 Forearm.seek :retract @14:05:05.78 Elbow.reconfigure TightElbow @14:05:05.83 Elbow.seek :garage @14:05:08.95 Elbow.reconfigure LooseElbow @14:05:09.05 Forearm.reconfigure TightForearm @14:05:09.10 Forearm.seek :garage @14:05:10.26 Hand.open @14:05:10.90 Forearm.reconfigure LooseForearm @14:05:10.94 Forearm.seek :retract @14:05:12.07 Starting mfb @14:05:12.09 pcrBAC wcr sampling at most 1500ml @14:05:12.18 Loading sh1 puck from tube 2 into Collection Clamp @14:05:12.27 Readying the MFB @14:05:12.33 Homing MFB Syringe @14:05:12.35 AFV.select :holdingCoil @14:05:12.58 ATV.dial :air2 @14:05:12.71
SC.reconfigure SCloose @14:05:12.94 Elbow.reconfigure TightElbow @14:05:12.99 Elbow.seek 2 @14:05:13.17 AS.reconfigure ASconfig @14:05:13.22 AS.home.seek :home @14:05:15.05
Forearm.reconfigure TightForearm @14:05:15.10 Forearm.seek 2 + 700 counts @14:05:16.12 SE.reconfigure SEpick @14:05:16.19 SE.seek beamBreak @14:05:17.68 SE.configure SEconfig @14:05:17.84 Elbow.seek 2 @14:05:18.05 Forearm.seek 2 + 300 counts @14:05:18.30 Forearm.reconfigure LooseForearm @14:05:18.34 Hand.close @14:05:18.75 AFV.select :carrierSolution @14:05:18.87 delay 2 seconds @14:05:18.97 SE.seek :down @14:05:19.22
Forearm.seek :retract @14:05:20.17 Elbow.seek CC @14:05:20.33 SE.coast @14:05:20.89 AS.seek 1ml @14:05:21.88
Elbow.reconfigure LooseElbow @14:05:21.99 Forearm.reconfigure TightForearm @14:05:22.04 Forearm.seek CC @14:05:23.87 CC.seek :closed @14:05:28.12 Collection Clamp onSpring/offSpring for 6/32 samples, on/off torque 1.74 @14:05:28.13 Hand.open @14:05:28.77 Forearm.reconfigure LooseForearm @14:05:28.82 Forearm.seek :retract @14:05:29.78 SC.coast @14:05:29.82 skipping prime and sample collection @14:05:29.83 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @14:05:29.84 Intake.select :close @14:05:29.92 CSV.dial :waste @14:05:30.01 CS.seek 0ml @14:05:30.10 CSV.dial :air @14:05:30.17
Calibrating pressure and temperature sensors @14:05:30.22 CTV.dial :air @14:05:30.23 SSV.dial :spit,avoiding: :exhaust @14:05:30.25 SS.reconfigure SSfast @14:05:30.30 SS.seek 5ml @14:05:30.90 CS.seek 10ml @14:05:33.14 SSV.dial CBV @14:05:33.17
CBV.dial SSV @14:05:34.46 delay 3.8 seconds @14:05:38.92 SSV.dial :spit @14:05:40.22 SS.seek 0ml @14:05:42.80 SSV.dial CBV @14:05:44.09 SSV.dial CBV,avoiding: :exhaust @14:05:44.12
CBV.dial SSV @14:05:44.14 CTV.dial :air @14:05:44.15 delay 15 seconds @14:05:59.19 SSV.dial :spit,avoiding: :exhaust @14:06:00.48 CTV.dial CSV,avoiding: :intake @14:06:00.52
SS.reconfigure SSexhale @14:06:00.63 SS.seek 0ml @14:06:00.75 SSV.dial CBV,avoiding: :exhaust @14:06:02.09 CBV.dial between SSV and waste2 @14:06:02.49 CTV.dial CSV,avoiding: :air @14:06:02.52
CSV.dial CTV @14:06:02.95 CS.reconfigure CSpressurize @14:06:03.00 CS.seek 0ml @14:06:13.87 delay 1 second @14:06:14.88 AFV.select :holdingCoil @14:06:14.99 delay 1 second @14:06:16.02 ATV.dial :waste @14:06:17.00 AS.reconfigure ASnorm @14:06:17.05 AS.home.seek :empty @14:06:29.21
CSV.dial :air @14:06:29.67 CS.reconfigure CSfast @14:06:29.72 CS.seek 10ml @14:06:51.69 CSV.dial between air and CTV @14:06:52.03 CS.seek 3.416ml @14:07:06.94 CTV.dial CSV,avoiding: :air @14:07:06.97
CSV.dial CTV @14:07:07.28 CS.reconfigure CSpressurize @14:07:07.33 CS.seek 0ml @14:07:09.61 ATV.dial :waste @14:07:09.63 ARV.dial between carrier and air1 @14:07:09.72 ASV.dial :waste1 @14:07:16.74
CSV.dial :air @14:07:17.19 CS.reconfigure CSfast @14:07:17.24 CS.seek 10ml @14:07:39.69 CSV.dial between air and CTV @14:07:40.03 CS.seek 2.149ml @14:07:57.38 CTV.dial CSV,avoiding: :air @14:07:57.41
CSV.dial CTV @14:07:57.72 CS.reconfigure CSpressurize @14:07:57.77 CS.seek 0ml @14:07:59.89 BubblePoint: Intake @69.6psia, Outlet @14.6psia @14:07:59.90 CBV.dial SSV @14:08:00.22 SS.reconfigure SSconfig @14:08:00.27 SS.seek 10ml @14:08:06.18 delay 15 seconds @14:08:21.32 Evacuated: Intake @28.4psia, Outlet @28.8psia @14:08:21.34 SSV.dial :spit,avoiding: :exhaust @14:08:22.64 SS.reconfigure SSexhale @14:08:22.69 SS.seek 0ml @14:08:27.55 SS.reconfigure SSfast @14:08:27.60 SS.seek 5ml @14:08:30.26 SSV.dial CBV,avoiding: :exhaust @14:08:30.29
CBV.dial SSV @14:08:31.59 CTV.dial :air,avoiding: :intake @14:08:32.89 CSV.dial CTV @14:08:32.93
SS.seek 10ml @14:08:33.92 CSV.dial :air @14:08:34.38 CS.reconfigure CSfast @14:08:34.43 CS.seek 0ml @14:08:35.57
SSV.dial :spit,avoiding: :exhaust @14:08:36.87 SS.reconfigure SSexhale @14:08:36.92 SS.seek 0ml @14:08:38.84 CS.coast @14:08:42.12
SS.reconfigure SSfast @14:08:42.17 SS.seek 5ml @14:08:44.65 SSV.dial CBV,avoiding: :exhaust @14:08:44.68
CBV.dial SSV @14:08:46.97 SSV.dial :spit,avoiding: :exhaust @14:08:48.26 SS.reconfigure SSconfig @14:08:48.31 SS.seek 0ml @14:08:51.36 SS.coast @14:08:51.41 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @14:08:51.42 Intake.select :close @14:08:51.50 CSV.dial :waste @14:08:51.63 SSV.dial CBV,avoiding: :exhaust @14:08:51.66
CBV.dial SSV @14:08:52.28 CS.seek 0ml @14:08:52.37 CSV.dial :air @14:08:52.95
CTV.dial :air @14:08:52.96 delay 15 seconds @14:08:53.10 CS.seek 10ml @14:09:07.99
SSV.dial :spit,avoiding: :exhaust @14:09:09.28 CTV.dial CSV,avoiding: :intake @14:09:09.32
SS.reconfigure SSexhale @14:09:09.43 SS.seek 0ml @14:09:09.49 SSV.dial CBV,avoiding: :exhaust @14:09:15.03 CBV.dial between SSV and waste2 @14:09:15.45 CTV.dial CSV,avoiding: :air @14:09:15.48
CSV.dial CTV @14:09:15.92 CS.reconfigure CSpressurize @14:09:15.97 CS.seek 0ml @14:09:42.18 CSV.dial :air @14:09:42.64 CS.reconfigure CSfast @14:09:42.69 CS.seek 10ml @14:10:04.99 CSV.dial between air and CTV @14:10:05.34 CS.seek 3.39ml @14:10:20.28 CTV.dial CSV,avoiding: :air @14:10:20.31
CSV.dial CTV @14:10:20.63 CS.reconfigure CSpressurize @14:10:20.68 CS.seek 0ml @14:10:30.02 CSV.dial :air @14:10:30.47 CS.reconfigure CSfast @14:10:30.52 CS.seek 10ml @14:10:52.35 CSV.dial between air and CTV @14:10:52.69 CS.seek 2.142ml @14:11:04.25 GoodNews.email "At 14:00:23 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tundeployv2.rb:110\n{1 => 6, 2 => 16}\nCan@14:00:23, 14.6C, 67% humidity, 14.3psia, 14.699V, 0.682A, 0.676A avg, 10.03W",Subject: "Started 18tundeployv2 phase coreNEG" @14:11:44.36 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:11:44.41 CTV.dial CSV,avoiding: :air @14:11:44.44 Retry #2 of 10 after 10:00 @14:11:44.46
CSV.dial CTV @14:11:44.77 CS.reconfigure CSpressurize @14:11:44.82 CS.seek 0ml @14:11:47.08 BubblePoint: Intake @70.4psia, Outlet @15.1psia @14:11:47.09 CBV.dial SSV @14:11:47.43 SS.reconfigure SSconfig @14:11:47.48 SS.seek 10ml @14:11:53.40 delay 15 seconds @14:12:08.54 Evacuated: Intake @28.4psia, Outlet @28.8psia @14:12:08.56 SSV.dial :spit,avoiding: :exhaust @14:12:09.84 SS.reconfigure SSexhale @14:12:09.89 SS.seek 0ml @14:12:14.99 SS.reconfigure SSfast @14:12:15.04 SS.seek 5ml @14:12:17.51 SSV.dial CBV,avoiding: :exhaust @14:12:17.54
CBV.dial SSV @14:12:18.81 CTV.dial :air,avoiding: :intake @14:12:20.07 CSV.dial CTV @14:12:20.12
SS.seek 10ml @14:12:21.11 CSV.dial :air @14:12:21.57 CS.reconfigure CSfast @14:12:21.62 CS.seek 0ml @14:12:22.76
SSV.dial :spit,avoiding: :exhaust @14:12:24.04 SS.reconfigure SSexhale @14:12:24.11 SS.seek 0ml @14:12:25.83 CS.coast @14:12:29.11
SS.reconfigure SSfast @14:12:29.16 SS.seek 5ml @14:12:31.81 SSV.dial CBV,avoiding: :exhaust @14:12:31.84
CBV.dial SSV @14:12:34.16 SSV.dial :spit,avoiding: :exhaust @14:12:35.45 SS.reconfigure SSconfig @14:12:35.50 SS.seek 0ml @14:12:38.77 SS.coast @14:12:38.81 Lysing: Adding 1.5ml lysis @14:12:38.83 CBV.dial between CSV and waste1,avoiding: :DBV @14:12:40.26 CTV.dial CSV @14:12:41.52 CSV.dial CSR @14:12:42.28 Pulling 1ml of CSR.lysis @14:12:42.29 CSR.select :lysis @14:12:42.40 CS.reconfigure CSconfig @14:12:42.45 CS.seek 1ml @14:12:45.97 delay 5 seconds @14:12:50.99 CSR.select :air @14:12:51.16 CS.seek 6ml @14:13:04.88 delay 2 seconds @14:13:06.89 CSV.dial :waste1 @14:13:08.13 CS.reconfigure CSfast @14:13:08.18 CS.seek :empty @14:13:21.59 CSV.dial CSR @14:13:23.39 Pulling 1.5ml of CSR.lysis @14:13:23.40 CSR.select :lysis @14:13:23.52 CS.reconfigure CSconfig @14:13:23.56 CS.seek 1.5ml @14:13:28.44 delay 5 seconds @14:13:33.46 CSR.select :air @14:13:33.64 CS.seek 4.501ml @14:13:42.29 delay 2 seconds @14:13:44.30 CSV.dial CTV @14:13:44.77 CS.seek 1.275ml @14:13:53.98 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @14:13:54.00 CH.seek 85C,8:00 @14:16:24.33 CH.hold 5:00,85C @14:21:24.51 Collection Puck Heater at 85C after lysing @14:21:24.53 CS.seek 0ml @14:21:28.92 CTV.dial between spare and air,avoiding: :intake @14:21:29.93 CSV.dial :air @14:21:30.38 CS.seek 5ml @14:21:44.03 CSV.dial CTV @14:21:44.48 delay 2 seconds @14:21:44.50 GoodNews.email "At 14:00:23 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tundeployv2.rb:110\n{1 => 6, 2 => 16}\nCan@14:00:23, 14.6C, 67% humidity, 14.3psia, 14.699V, 0.682A, 0.676A avg, 10.03W",Subject: "Started 18tundeployv2 phase coreNEG" @14:22:24.61 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:22:24.62 Retry #3 of 10 after 10:00 @14:22:24.65
CSV.dial :air @14:22:25.09 delay 2 seconds @14:22:27.10 CSV.dial CTV @14:22:27.56 delay 2 seconds @14:22:29.57 CSV.dial :air @14:22:30.03 delay 2 seconds @14:22:32.05 CS.seek 0ml @14:22:45.76 Recovering Lysate into Collection syringe @14:22:45.77 CSV.dial CBV @14:22:46.74 CBV.dial CSV @14:22:47.13 CS.seek 5ml @14:23:00.95 delay 5 seconds @14:23:05.96 CTV.dial :air @14:23:06.49 CS.seek 7ml @14:23:12.55 delay 5 seconds @14:23:17.57 CSV.dial :air2 @14:23:18.01 CS.seek 10ml @14:23:26.72 CSV.dial between air and CTV,avoiding: CBV @14:23:27.83 Lysing Complete @14:23:27.91 Storing sh1 puck in Collection Clamp to tube 1 @14:23:28.09 SC.reconfigure SCtight @14:23:28.14 SC.seek 1 @14:23:30.28 SC.reconfigure SCloose @14:23:30.51 Elbow.reconfigure TightElbow @14:23:30.56 Elbow.seek 1 @14:23:32.48 Forearm.reconfigure TightForearm @14:23:33.06 Forearm.seek 1 + 700 counts @14:23:34.17 SE.reconfigure SEpick @14:23:34.25 SE.seek beamBreak @14:23:36.85 SE.configure SEconfig @14:23:37.03 Elbow.reconfigure LimpElbow @14:23:37.08 Forearm.reconfigure LooseForearm @14:23:37.13 Forearm.seek 1 @14:23:37.44 Elbow.hold @14:23:37.51 Elbow.reconfigure TightElbow @14:23:37.62 SE.seek 5.09 Pucks below Top Plate @14:23:38.09 Forearm.force -20 @14:23:38.14 Forearm.seek 1 + 344 counts @14:23:38.46 Forearm.reconfigure TightForearm @14:23:38.51 Forearm.seek 1 + 700 counts @14:23:38.78 SE.seek 6.09 Pucks below Top Plate @14:23:39.44 Forearm.reconfigure LooseForearm @14:23:39.49 Forearm.seek :retract @14:23:40.42 Elbow.seek CC @14:23:42.13 Elbow.reconfigure LooseElbow @14:23:42.24 Forearm.seek CC @14:23:43.22 Hand.close @14:23:43.86 CC.seek :open @14:23:48.35 Forearm.seek :retract @14:23:49.39 Elbow.reconfigure TightElbow @14:23:49.44 Elbow.seek 1 @14:23:51.37 Elbow.reconfigure LimpElbow @14:23:51.43 Forearm.seek 1 @14:23:52.45 Elbow.hold @14:23:52.52 Elbow.reconfigure TightElbow @14:23:52.57 Hand.open @14:23:53.37 Forearm.force -20 @14:23:53.41 Forearm.seek 1 + 350 counts @14:23:53.72 Forearm.reconfigure TightForearm @14:23:53.77 Forearm.seek 1 + 700 counts @14:23:54.09 SE.seek :down @14:23:55.25
Forearm.reconfigure LooseForearm @14:23:55.29 Forearm.seek :retract @14:23:56.30 SE.coast @14:23:56.34
SC.coast @14:23:56.38 Tranferring lysate to PRV @14:23:56.39 CSV.dial :air @14:23:56.70 CS.seek 10ml @14:23:56.79 CSV.dial between air and CTV @14:23:57.12 AIV.dial :bypass @14:23:57.31 Beginning samplePassC @14:23:57.32 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:23:57.40 Hand.open @14:23:58.25 Elbow.seek FlushPuck::Garage @14:24:00.15 Elbow.reconfigure LooseElbow @14:24:00.26 Forearm.seek :garage @14:24:01.25 Hand.close @14:24:01.99 Forearm.seek :retract @14:24:03.01 Elbow.reconfigure TightElbow @14:24:03.06 Elbow.seek CC @14:24:05.99 Elbow.reconfigure LooseElbow @14:24:06.10 Forearm.reconfigure TightForearm @14:24:06.15 Forearm.seek CC @14:24:07.45 CC.seek :closed @14:24:11.68 Collection Clamp onSpring/offSpring for 6/32 samples, on/off torque 1.73 @14:24:11.77 Hand.open @14:24:12.43 Forearm.reconfigure LooseForearm @14:24:12.47 Forearm.seek :retract @14:24:13.47 AWV.select :air @14:24:13.49 AIV.dial :bypass @14:24:13.50 PSV.dial PSR @14:24:14.85 Pulling 1.5ml of PSR.flush PSR.select :flush @14:24:14.98 PS.seek 1.5ml @14:24:31.00 delay 5 seconds @14:24:36.02 PSV.dial :waste1 @14:24:36.48 PS.seek 0ml @14:24:52.52 PSV.dial PSR @14:24:52.96 PSR.select :air @14:24:53.09 PS.seek 2.5ml @14:25:19.28 PRV.dial CBV @14:25:19.31
CBV.dial PRV @14:25:20.10 PSV.dial :mixloop @14:25:21.09 CTV.dial :air,avoiding: :intake @14:25:21.10 PTV.dial :PRVmixing @14:25:21.19 PS.seek 0ml @14:25:47.27 PSV.dial :air @14:25:47.73 PS.seek 2.5ml @14:26:13.86 PSV.dial :mixloop @14:26:14.31 PS.seek 0ml @14:26:40.49 PSV.dial :air @14:26:40.94 PS.seek 2.5ml @14:27:07.08 PSV.dial :mixloop @14:27:07.53 PS.seek 0ml @14:27:33.75 Garaging FlushPuck in Collection Clamp @14:27:33.83 Elbow.reconfigure TightElbow @14:27:33.88 Elbow.seek CC @14:27:34.15 Elbow.reconfigure LooseElbow @14:27:34.26 Forearm.seek CC @14:27:35.26 Hand.close @14:27:35.86 CC.seek :open @14:27:40.21 Forearm.seek :retract @14:27:41.19 Elbow.reconfigure TightElbow @14:27:41.24 Elbow.seek :garage @14:27:44.89 Elbow.reconfigure LooseElbow @14:27:45.00 Forearm.reconfigure TightForearm @14:27:45.05 Forearm.seek :garage @14:27:46.21 Hand.open @14:27:46.93 Forearm.reconfigure LooseForearm @14:27:46.97 Forearm.seek :retract @14:27:48.02 Connecting PS to CS to transfer sample @14:27:48.03 CBV.dial AIV @14:27:48.04 AIV.dial :bypass @14:27:48.05 PSV.dial :mixloop @14:27:48.07 PTV.dial :PRVmixing @14:27:48.08 PRV.dial AIV @14:27:48.09 CTV.dial :air,avoiding: :intake @14:27:48.11 Loading lyfil puck from tube 2 into Collection Clamp @14:27:48.36 SC.reconfigure SCtight @14:27:48.41 SC.seek 2 @14:27:50.55 SC.reconfigure SCloose @14:27:50.78 Elbow.reconfigure TightElbow @14:27:50.83 Elbow.seek 2 @14:27:53.01 Forearm.reconfigure TightForearm @14:27:53.06 Forearm.seek 2 + 700 counts @14:27:54.09 SE.reconfigure SEpick @14:27:54.16 SE.seek beamBreak @14:27:55.86 SE.configure SEconfig @14:27:56.03 Elbow.seek 2 @14:27:56.30 Forearm.seek 2 + 300 counts @14:27:56.56 Forearm.reconfigure LooseForearm @14:27:56.60 Hand.close @14:27:57.25 SE.seek :down @14:27:57.48
Forearm.seek :retract @14:27:58.44 Elbow.seek CC @14:27:58.74 SE.coast @14:28:00.00
Elbow.reconfigure LooseElbow @14:28:00.11 Forearm.reconfigure TightForearm @14:28:00.16 Forearm.seek CC @14:28:01.45 CC.seek :closed @14:28:05.75 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.76 @14:28:05.76 Hand.open @14:28:06.43 Forearm.reconfigure LooseForearm @14:28:06.48 Forearm.seek :retract @14:28:07.45 SC.coast @14:28:07.49 CTV.dial CSV,avoiding: :intake @14:28:08.77 CSV.dial CTV @14:28:09.09 CS.seek 5ml @14:28:22.86 delay 1:20 @14:29:42.89 CS.seek 2.5ml @14:29:50.26 delay 40 seconds @14:30:30.30 CS.seek 0ml @14:30:37.65 delay 1:00 @14:31:37.67 PSV.dial :air @14:31:38.14 PS.seek 2.5ml @14:32:04.43 PSV.dial :mixloop @14:32:04.88 delay 3 seconds @14:32:07.89 PSV.dial :air @14:32:08.35 delay 2 seconds @14:32:10.36 PSV.dial :mixloop @14:32:11.35 delay 3 seconds @14:32:14.36 PSV.dial :waste1 @14:32:15.64 PS.seek :empty @14:32:24.65 GoodNews.email "At 14:00:23 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tundeployv2.rb:110\n{1 => 6, 2 => 16}\nCan@14:00:23, 14.6C, 67% humidity, 14.3psia, 14.699V, 0.682A, 0.676A avg, 10.03W",Subject: "Started 18tundeployv2 phase coreNEG" @14:33:04.76 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:33:04.80
CBV.dial :waste1 @14:33:04.82 Retry #4 of 10 after 10:00 @14:33:05.30
delay 5 seconds @14:33:10.32 CBV.dial AIV @14:33:10.79 delay 10 seconds @14:33:20.82 CBV.dial :waste1 @14:33:21.29 delay 10 seconds @14:33:31.31 CBV.dial AIV @14:33:31.77 delay 10 seconds @14:33:41.80 CTV.dial CSV,avoiding: :intake @14:33:41.81 CBV.dial between AIV and waste1 @14:33:42.16 CSV.dial :air @14:33:42.73 CS.seek 10ml @14:34:09.26 CSV.dial CTV @14:34:09.70 CS.seek 0ml @14:34:36.20 CBV.dial :waste1 @14:34:36.52 delay 10 seconds @14:34:46.55 CTV.dial :air,avoiding: :intake @14:34:47.87 Storing lyfil puck in Collection Clamp to tube 1 @14:34:48.06 SC.reconfigure SCtight @14:34:48.11 SC.seek 1 @14:34:50.22 SC.reconfigure SCloose @14:34:50.46 Elbow.reconfigure TightElbow @14:34:50.50 Elbow.seek 1 @14:34:52.42 Forearm.reconfigure TightForearm @14:34:52.47 Forearm.seek 1 + 700 counts @14:34:53.47 SE.reconfigure SEpick @14:34:53.54 SE.seek beamBreak @14:34:55.95 SE.configure SEconfig @14:34:56.12 Elbow.reconfigure LimpElbow @14:34:56.17 Forearm.reconfigure LooseForearm @14:34:56.21 Forearm.seek 1 @14:34:56.54 Elbow.hold @14:34:56.61 Elbow.reconfigure TightElbow @14:34:56.72 SE.seek 6.08 Pucks below Top Plate @14:34:57.27 Forearm.force -20 @14:34:57.32 Forearm.seek 1 + 318 counts @14:34:57.64 Forearm.reconfigure TightForearm @14:34:57.69 Forearm.seek 1 + 700 counts @14:34:57.95 SE.seek 7.08 Pucks below Top Plate @14:34:58.59 Forearm.reconfigure LooseForearm @14:34:58.63 Forearm.seek :retract @14:34:59.59 Elbow.seek CC @14:35:01.29 Elbow.reconfigure LooseElbow @14:35:01.39 Forearm.seek CC @14:35:02.36 Hand.close @14:35:03.00 CC.seek :open @14:35:07.53 Forearm.seek :retract @14:35:08.50 Elbow.reconfigure TightElbow @14:35:08.55 Elbow.seek 1 @14:35:10.49 Elbow.reconfigure LimpElbow @14:35:10.55 Forearm.seek 1 @14:35:11.57 Elbow.hold @14:35:12.16 Elbow.reconfigure TightElbow @14:35:12.22 Hand.open @14:35:13.01 Forearm.force -20 @14:35:13.05 Forearm.seek 1 + 347 counts @14:35:13.36 Forearm.reconfigure TightForearm @14:35:13.41 Forearm.seek 1 + 700 counts @14:35:13.73 SE.seek :down @14:35:14.88
Forearm.reconfigure LooseForearm @14:35:14.93 Forearm.seek :retract @14:35:15.83 SE.coast @14:35:15.90
SC.coast @14:35:15.94 PSV.dial :air @14:35:17.00 PS.seek 0.499ml @14:35:22.88 delay 3 seconds @14:35:25.90 PSV.dial :mixloop @14:35:26.32 PRV.dial :air @14:35:26.79 delay 3 seconds @14:35:29.81 PRV.dial CBV @14:35:30.25 CBV.dial AIV @14:35:30.73 PRV.dial between CBV and air @14:35:31.05 PRV.dial :air @14:35:31.42 PS.reconfigure PSslow @14:35:31.47 PS.seek 0.599ml @14:35:34.00 PRV.dial :waste2 @14:35:34.71 PS.reconfigure PSconfig @14:35:34.76 PS.seek :empty @14:35:41.66 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:35:41.73 Hand.open @14:35:42.13 Elbow.seek FlushPuck::Garage @14:35:44.10 Elbow.reconfigure LooseElbow @14:35:44.22 Forearm.seek :garage @14:35:45.25 Hand.close @14:35:46.00 Forearm.seek :retract @14:35:47.04 Elbow.reconfigure TightElbow @14:35:47.09 Elbow.seek CC @14:35:50.02 Elbow.reconfigure LooseElbow @14:35:50.13 Forearm.reconfigure TightForearm @14:35:50.18 Forearm.seek CC @14:35:51.48 CC.seek :closed @14:35:55.73 Collection Clamp onSpring/offSpring for 5/32 samples, on/off torque 1.70 @14:35:55.81 Hand.open @14:35:56.57 Forearm.reconfigure LooseForearm @14:35:56.61 Forearm.seek :retract @14:35:57.62 Clearing PML for modified lysate @14:35:57.63 CTV.dial :air,avoiding: :intake @14:35:57.65 PRV.dial :waste2 @14:35:57.66 PTV.dial :PRV,avoiding: :puck @14:35:58.38 delay 2 seconds @14:36:00.40 PTV.dial :PRVmixing,avoiding: :puck @14:36:01.68 delay 2 seconds @14:36:03.69 PSV.dial :PML @14:36:03.71 delay 2 seconds @14:36:05.73 PRV.dial :air @14:36:06.46 delay 2 seconds @14:36:08.54 PS.seek 1.499ml @14:36:24.63 delay 5 seconds @14:36:29.66 PS.seek 2.5ml @14:36:40.68 PSV.dial :air @14:36:41.13 PS.seek 0ml @14:37:07.32 mixing lysate with speDil @14:37:07.33 PSV.dial :PML @14:37:07.77 CBV.dial :PRV @14:37:07.78 PRV.dial :air @14:37:07.79 delay 2 seconds @14:37:09.81 PRV.dial :speDil @14:37:10.27 delay 2 seconds @14:37:12.36 PS.reconfigure PSslow1 @14:37:12.41 PS.seek 0.225ml @14:37:35.94 delay 5 seconds @14:37:40.96 PRV.dial CBV @14:37:41.69 delay 2 seconds @14:37:43.77 PS.seek 0.475ml @14:38:09.44 delay 5 seconds @14:38:14.46 PRV.dial :air @14:38:14.94 delay 2 seconds @14:38:16.96 PS.seek 0.965ml @14:39:06.91 delay 10 seconds @14:39:16.94 PRV.dial CBV @14:39:17.38 CTV.dial :air,avoiding: :intake @14:39:17.39 PSV.dial :air @14:39:17.84 PS.reconfigure PSconfig @14:39:17.89 PS.seek 2.5ml @14:39:34.29 delay 3 seconds @14:39:37.30 PSV.dial PTV @14:39:38.02 PTV.dial :PRV,avoiding: :puck @14:39:38.74 PS.seek 0ml @14:40:04.81 PSV.dial :air @14:40:05.52 PS.seek 2.5ml @14:40:31.66 delay 3 seconds @14:40:34.68 PSV.dial PTV @14:40:35.38 PTV.dial :PRV,avoiding: :puck @14:40:35.40 PS.seek 0ml @14:41:01.48 Pushing modified lysate into AIV Injection Loop @14:41:01.49 CBV.dial AIV @14:41:01.50 AIV.dial :bypass @14:41:01.51 PRV.dial CBV @14:41:01.52 PSV.dial :air @14:41:02.24 PS.seek 2.5ml @14:41:28.45 delay 3 seconds @14:41:31.46 PSV.dial :PML @14:41:31.92 PTV.dial :PRVmixing,avoiding: :puck @14:41:32.70 PS.reconfigure PSslow1 @14:41:32.75 PS.seek 1.74ml @14:42:50.13 delay 5 seconds @14:42:55.15 AIV.dial :sample @14:42:55.69 AWV.select :waste @14:42:55.87 PS.reconfigure PSslow2 @14:42:55.92 PS.seek 1.22ml @14:43:04.86 GoodNews.email "At 14:00:23 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tundeployv2.rb:110\n{1 => 6, 2 => 16}\nCan@14:00:23, 14.6C, 67% humidity, 14.3psia, 14.699V, 0.682A, 0.676A avg, 10.03W",Subject: "Started 18tundeployv2 phase coreNEG" @14:43:44.97 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:43:44.98 Retry #5 of 10 after 10:00 @14:44:44.35
delay 15 seconds @14:44:59.37 AIV.dial :bypass @14:44:59.94 PS.reconfigure PSconfig @14:44:59.99 PS.seek 0ml @14:45:13.19 PSV.dial :air @14:45:13.64 PS.seek 2.5ml @14:45:39.84 PSV.dial :PML @14:45:40.29 PS.seek 0ml @14:46:06.41 AIV.dial :coilToMFB @14:46:06.43 AWV.select :air @14:46:06.60 Extracting Nucleic Acid from AIV @14:46:06.63
Beginning samplePassD @14:46:06.66 SPE.hold 2:46:40,55C @14:46:06.74 ASV.dial :SPEdelivery @14:46:06.84
AWV.select :air @14:46:06.85 AIV.dial :bypass @14:46:06.86 PSV.dial PSR @14:46:07.45 delay 20 seconds @14:46:08.48
Pulling 1.5ml of PSR.killMFB @14:46:08.49 PSR.select :killMFB @14:46:08.62 PS.seek 1.5ml @14:46:24.73 delay 5 seconds @14:46:27.48 primeLysateINT @14:46:27.49 AWV.select :air @14:46:27.50 AIV.dial :coilToMFB @14:46:27.51 ATV.dial AIV @14:46:29.11 AS.reconfigure ASslow1 @14:46:29.16 AS.seek 0.299ml @14:46:29.74
PSV.dial :waste1 @14:46:30.20 PS.seek 0ml @14:46:46.19 PSV.dial PSR @14:46:46.78 PSR.select :air @14:46:46.92 PS.seek 2.5ml @14:47:13.01 PRV.dial CBV @14:47:13.04
CBV.dial PRV @14:47:13.05 PSV.dial :mixloop @14:47:14.07 CTV.dial :air,avoiding: :intake @14:47:14.08 PTV.dial :PRVmixing @14:47:14.10 PS.seek 0ml @14:47:29.82 delay 10 seconds @14:47:39.84 ATV.dial :air2 @14:47:40.28
PSV.dial PSR @14:47:40.64 AS.seek 0.329ml @14:47:41.35
Pulling 1.5ml of PSR.flush @14:47:41.36 PSR.select :flush @14:47:41.49 PS.seek 1.5ml @14:47:46.97 ATV.dial :waste2 @14:47:47.51 AS.reconfigure ASnorm @14:47:47.56 AS.seek :empty @14:47:57.45
delay 5 seconds @14:48:02.47 PSV.dial :waste1 @14:48:02.96 PS.seek 0ml @14:48:04.55 Lysate primed! @14:48:04.56 AFV.select :holdingCoil @14:48:04.57 ATV.dial :air2 @14:48:05.17 AS.reconfigure ASslow1 @14:48:05.22 AS.seek 0.069ml @14:48:18.94
PSV.dial PSR @14:48:19.40 PSR.select :air @14:48:19.57 PS.seek 2.5ml @14:48:19.63 delay 3 seconds @14:48:22.66 ATV.dial :AIV @14:48:23.56 AS.seek 0.469ml @14:48:45.70 PRV.dial CBV @14:48:45.73
CBV.dial PRV @14:48:45.74 PSV.dial :mixloop @14:48:46.73 CTV.dial :air,avoiding: :intake @14:48:46.74 PTV.dial :PRVmixing @14:48:46.76 PS.seek 0ml @14:49:12.85 PSV.dial PSR @14:49:13.92 Pulling 1.5ml of PSR.flush @14:49:13.93 PSR.select :flush @14:49:14.06 PS.seek 1.5ml @14:49:30.04 delay 5 seconds @14:49:35.07 PSV.dial :waste1 @14:49:35.65 PS.seek 0ml @14:49:44.27 delay 5 seconds @14:49:49.36 Solid Phase Extraction Heater at 51.3C @14:49:49.37 ATV.dial ASV @14:49:49.75 ASV.dial :SPEdelivery @14:49:49.84 AS.seek 0.041ml @14:49:51.66
PSV.dial PSR @14:49:52.11 PSR.select :air @14:49:52.24 PS.seek 2.5ml @14:50:18.36 PRV.dial CBV @14:50:18.39
CBV.dial PRV @14:50:18.40 PSV.dial :mixloop @14:50:19.39 CTV.dial :air,avoiding: :intake @14:50:19.40 PTV.dial :PRVmixing @14:50:19.42 PS.seek 0ml @14:50:45.60 PSV.dial PSR @14:50:46.65 Pulling 1.5ml of PSR.flush @14:50:46.66 PSR.select :flush @14:50:46.79 PS.seek 1.5ml @14:51:03.38 delay 5 seconds @14:51:08.47 PSV.dial :waste1 @14:51:08.92 PS.seek 0ml @14:51:16.20 delay 10 seconds @14:51:25.00
PSV.dial PSR @14:51:25.46 PSR.select :air @14:51:25.60 PS.seek 2.5ml @14:51:26.22 rinseLysateLineINT @14:51:26.23 AFV.select :carrierSolution @14:51:26.34 delay 2 seconds @14:51:28.37 AS.reconfigure ASconfig @14:51:28.42 AS.seek 1ml @14:51:51.73 PRV.dial CBV @14:51:51.76
CBV.dial PRV @14:51:51.77 PSV.dial :mixloop @14:51:52.75 CTV.dial :air,avoiding: :intake @14:51:52.76 PTV.dial :PRVmixing @14:51:52.78 PS.reconfigure PSslow @14:51:52.83 PS.seek 1.2ml @14:52:19.67 PSV.dial :waste1 @14:52:20.94 PS.reconfigure PSconfig @14:52:20.99 PS.seek 0ml @14:52:23.87 delay 1 second @14:52:24.88 AFV.select :holdingCoil @14:52:25.00 delay 1 second @14:52:26.02 ATV.dial AIV @14:52:26.56 AIV.dial :coilToMFB @14:52:26.57 AWV.select :waste @14:52:26.68 AS.reconfigure ASslow0 @14:52:26.73 AS.seek 0ml @14:52:34.01
PSV.dial :air @14:52:35.13 PS.seek 2.5ml @14:53:01.32 PSV.dial :mixloop @14:53:01.78 PS.reconfigure PSslow @14:53:01.83 PS.seek 0ml @14:53:45.00 GoodNews.email "At 14:00:23 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tundeployv2.rb:110\n{1 => 6, 2 => 16}\nCan@14:00:23, 14.6C, 67% humidity, 14.3psia, 14.699V, 0.682A, 0.676A avg, 10.03W",Subject: "Started 18tundeployv2 phase coreNEG" @14:54:25.11 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:54:25.15 Retry #6 of 10 after 10:00 @14:54:25.20 ATV.dial :air2 @14:54:25.22
PSV.dial :air @14:54:25.70 PS.reconfigure PSconfig @14:54:25.75 PS.seek 2.5ml @14:54:25.90 AS.reconfigure ASslow1 @14:54:25.95 AS.seek 0.05ml @14:54:36.30 ATV.dial :AIV @14:54:37.20 AS.seek 0.01ml @14:54:45.52 ATV.dial :waste @14:54:47.12 AS.seek :empty @14:54:49.56 ethanol wash @14:54:49.57 ATV.dial :air2 @14:54:50.75 AS.reconfigure ASslow2 @14:54:50.80 AS.seek 0.009ml @14:54:51.98
PSV.dial :mixloop @14:54:52.44 PS.reconfigure PSslow @14:54:52.49 PS.seek 0ml @14:55:01.23 delay 3 seconds @14:55:04.26 Pulling 0.4ml of Ethanol Wash @14:55:04.27 ATV.dial :ethanol @14:55:05.04 AS.reconfigure ASslow1 @14:55:05.09 AS.seek 0.409ml @14:55:43.80
Cleaning Collection Syringe @14:55:43.81 CSV.dial CSR @14:55:44.30 Pulling 4ml of CSR.flush @14:55:44.31 CSR.select :flush @14:55:44.44 CS.seek 4ml @14:55:55.57 delay 5 seconds @14:56:00.59 CSR.select :air @14:56:00.73 CS.seek 10ml @14:56:16.98 CSV.dial CTV @14:56:17.01
CTV.dial CSV @14:56:18.35 CBV.dial :waste1 @14:56:18.82 CS.seek 0ml @14:56:25.82 delay 3 seconds @14:56:28.84 ATV.dial :air1 @14:56:29.23 delay 3 seconds @14:56:32.31 AS.seek 0.434ml @14:56:38.35 delay 3 seconds @14:56:41.37 ATV.dial :ethanol @14:56:41.97 AS.seek 0.419ml @14:56:45.26 delay 3 seconds @14:56:45.38
CSV.dial CSR @14:56:45.90 Pulling 4ml of CSR.flush @14:56:45.91 CSR.select :flush @14:56:46.04 CS.seek 4ml @14:56:48.27 ATV.dial ASV @14:56:49.52 AS.seek 0.092ml @14:56:57.76
delay 5 seconds @14:57:02.78 CSR.select :air @14:57:02.92 CS.seek 10ml @14:57:19.26 CSV.dial CTV @14:57:19.29
CTV.dial CSV @14:57:19.76 CBV.dial :waste1 @14:57:19.78 CS.seek 0ml @14:57:46.30 Garaging FlushPuck in Collection Clamp @14:57:46.38 Elbow.reconfigure TightElbow @14:57:46.43 Elbow.seek CC @14:57:46.70 Elbow.reconfigure LooseElbow @14:57:46.82 Forearm.seek CC @14:57:47.81 Hand.close @14:57:48.40 CC.seek :open @14:57:52.77 Forearm.seek :retract @14:57:53.76 Elbow.reconfigure TightElbow @14:57:53.81 Elbow.seek :garage @14:57:55.53 delay 3 seconds @14:57:56.85
Elbow.reconfigure LooseElbow @14:57:56.96 Forearm.reconfigure TightForearm @14:57:57.01 Forearm.seek :garage @14:57:58.19 Hand.open @14:57:58.54 ATV.dial :waste2 @14:57:58.85
Forearm.reconfigure LooseForearm @14:57:58.90 Forearm.seek :retract @14:57:59.26 AS.reconfigure ASconfig @14:57:59.31 AS.seek :empty @14:57:59.93 Whole Cell Archive sampling 1500ml @14:57:59.95 Loading wcr puck from tube 2 into Collection Clamp @14:58:00.21 SC.reconfigure SCtight @14:58:00.26 SC.seek 2 @14:58:02.45 SC.reconfigure SCloose @14:58:02.68 Elbow.reconfigure TightElbow @14:58:02.73 Elbow.seek 2 @14:58:04.75 Forearm.reconfigure TightForearm @14:58:04.81 Forearm.seek 2 + 700 counts @14:58:04.95 ATV.dial :air2 @14:58:05.32 AS.reconfigure ASslow1 @14:58:05.37 AS.seek 1ml @14:58:05.90 SE.reconfigure SEpick @14:58:05.97 SE.seek beamBreak @14:58:07.71 SE.configure SEconfig @14:58:07.88 Elbow.seek 2 @14:58:08.08 Forearm.seek 2 + 300 counts @14:58:08.42 Forearm.reconfigure LooseForearm @14:58:08.47 Hand.close @14:58:09.17 SE.seek :down @14:58:09.41 Forearm.seek :retract @14:58:10.39 Elbow.seek CC @14:58:10.77 SE.coast @14:58:12.06 Elbow.reconfigure LooseElbow @14:58:12.73 Forearm.reconfigure TightForearm @14:58:12.78 Forearm.seek CC @14:58:14.09 CC.seek :closed @14:58:18.34 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.79 @14:58:18.36 Hand.open @14:58:19.10 Forearm.reconfigure LooseForearm @14:58:19.15 Forearm.seek :retract @14:58:20.11 SC.coast @14:58:20.15 collectSample 1500ml w/bubble point of 55psi @14:58:20.16 Priming 7ml @14:58:20.17 Intake.select :close @14:58:20.19 Exhaust.select :close @14:58:20.20 SS.hold @14:58:20.44 Calibrating pressure sensors @14:58:20.45 CTV.dial :air @14:58:21.72 SSV.dial :spit,avoiding: :exhaust @14:58:21.74 SS.reconfigure SSfast @14:58:21.79 SS.seek 5ml @14:58:24.42 SSV.dial CBV @14:58:24.45 CBV.dial SSV @14:58:25.82 delay 3.8 seconds @14:58:29.77 SSV.dial :spit @14:58:31.11 SS.seek 0ml @14:58:33.89 SSV.dial CBV @14:58:35.20 CTV.dial :intake @14:58:35.93 SSV.dial CBV @14:58:35.96 CBV.dial SSV @14:58:35.99 Allowing 40s for 7.0ml of remaining 7.0ml @14:58:36.00 Intake.select :open @14:58:38.17 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:58:38.19 SS.reconfigure SSinhale.with maxSpeed: 150 @14:58:38.24 SS.seek 7ml @14:58:41.41 Vacuum too high -- waiting up to 40s @14:58:43.77 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:58:43.79 SS.reconfigure SSinhale.with maxSpeed: 135 @14:58:43.84 SS.seek 7ml @14:58:46.87 Stroke completed -- waiting for pressure to equalize ... @14:58:51.38 Intake.select :close @14:58:51.54 SSV.dial :exhaust @14:58:52.30 Exhaust.select :open @14:58:52.42 SS.reconfigure SSexhale @14:58:52.47 SS.seek 0ml @14:58:56.04 Exhaust.select :close @14:58:56.16 Sampling 1500.0ml @14:58:56.18 Intake.select :close @14:58:56.19 Exhaust.select :close @14:58:56.20 SS.reconfigure SSconfig @14:58:56.25 SS.hold @14:58:56.47 CTV.dial :intake @14:58:56.49 SSV.dial CBV @14:58:56.52 CBV.dial SSV @14:58:57.31 Allowing 130s for 25.0ml of remaining 1500.0ml @14:58:57.32 Intake.select :open @14:58:59.51 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:58:59.53 SS.reconfigure SSinhale.with maxSpeed: 135 @14:58:59.58 SS.seek 25ml @14:59:03.16 Vacuum too high -- waiting up to 40s @14:59:04.79 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:59:04.81 SS.reconfigure SSinhale.with maxSpeed: 122 @14:59:04.86 SS.seek 25ml @14:59:07.35 Vacuum too high -- waiting up to 40s @14:59:08.72 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:59:08.74 SS.reconfigure SSinhale.with maxSpeed: 110 @14:59:08.79 SS.seek 25ml @14:59:11.50 Vacuum too high -- waiting up to 40s @14:59:13.12 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:59:13.14 SS.reconfigure SSinhale.with maxSpeed: 99 @14:59:13.18 SS.seek 25ml @14:59:15.97 Vacuum too high -- waiting up to 40s @14:59:17.32 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:59:17.34 SS.reconfigure SSinhale.with maxSpeed: 89 @14:59:17.39 SS.seek 25ml @14:59:20.35 Vacuum too high -- waiting up to 40s @14:59:21.71 Intake @32.9psia, vacuum from 20.0 to 24.0psi @14:59:21.73 SS.reconfigure SSinhale.with maxSpeed: 80 @14:59:21.78 SS.seek 25ml @14:59:25.63 Vacuum too high -- waiting up to 40s @14:59:27.00 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:59:27.02 SS.reconfigure SSinhale.with maxSpeed: 72 @14:59:27.07 SS.seek 25ml @14:59:31.08 Vacuum too high -- waiting up to 40s @14:59:32.50 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:59:32.52 SS.reconfigure SSinhale.with maxSpeed: 65 @14:59:32.57 SS.seek 25ml @14:59:36.84 Stroke completed -- waiting for pressure to equalize ... @14:59:39.45 Intake.select :close @14:59:39.61 SSV.dial :exhaust @14:59:40.37 Exhaust.select :open @14:59:40.48 SS.reconfigure SSexhale @14:59:40.53 SS.seek 0ml @14:59:49.14 Exhaust.select :close @14:59:49.26 SSV.dial CBV @14:59:49.29 CBV.dial SSV @14:59:50.07 Allowing 130s for 25.0ml of remaining 1475.0ml @14:59:50.08 Intake.select :open @14:59:52.27 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:59:52.29 SS.reconfigure SSinhale.with maxSpeed: 65 @14:59:52.34 SS.seek 25ml @14:59:57.85 Vacuum too high -- waiting up to 40s @14:59:58.76 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:59:58.78 SS.reconfigure SSinhale.with maxSpeed: 59 @14:59:58.83 SS.seek 25ml @15:00:04.04 Vacuum too high -- waiting up to 40s @15:00:04.90 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:00:04.92 SS.reconfigure SSinhale.with maxSpeed: 53 @15:00:04.97 SS.seek 25ml @15:00:19.41 Vacuum too high -- waiting up to 40s @15:00:20.27 Intake @33.2psia, vacuum from 20.0 to 24.0psi @15:00:20.29 SS.reconfigure SSinhale.with maxSpeed: 48 @15:00:20.33 SS.seek 25ml @15:00:29.73 Stroke completed -- waiting for pressure to equalize ... @15:00:32.33 Intake.select :close @15:00:32.50 SSV.dial :exhaust @15:00:33.26 Exhaust.select :open @15:00:33.37 SS.reconfigure SSexhale @15:00:33.42 SS.seek 0ml @15:00:42.09 Exhaust.select :close @15:00:42.21 SSV.dial CBV @15:00:42.24 CBV.dial SSV @15:00:43.02 Allowing 130s for 25.0ml of remaining 1450.0ml @15:00:43.03 Intake.select :open @15:00:45.21 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:00:45.23 SS.reconfigure SSinhale.with maxSpeed: 48 @15:00:45.28 SS.seek 25ml @15:01:22.66 Stroke completed -- waiting for pressure to equalize ... @15:01:25.26 Intake.select :close @15:01:25.42 SSV.dial :exhaust @15:01:26.17 Exhaust.select :open @15:01:26.29 SS.reconfigure SSexhale @15:01:26.34 SS.seek 0ml @15:01:26.96 delay 5 seconds @15:01:31.98 ATV.dial ASV @15:01:32.90 ASV.dial :SPEdelivery @15:01:33.48 AS.seek 0.02ml @15:01:35.22 Exhaust.select :close @15:01:35.34 SSV.dial CBV @15:01:35.37 CBV.dial SSV @15:01:36.17 Allowing 130s for 25.0ml of remaining 1425.0ml @15:01:36.18 Intake.select :open @15:01:38.37 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:01:38.39 SS.reconfigure SSinhale.with maxSpeed: 48 @15:01:38.44 SS.seek 25ml @15:02:15.51 Stroke completed -- waiting for pressure to equalize ... @15:02:18.12 Intake.select :close @15:02:18.28 SSV.dial :exhaust @15:02:19.04 Exhaust.select :open @15:02:19.15 SS.reconfigure SSexhale @15:02:19.20 SS.seek 0ml @15:02:27.91 Exhaust.select :close @15:02:28.03 SSV.dial CBV @15:02:28.06 CBV.dial SSV @15:02:28.85 Allowing 130s for 25.0ml of remaining 1400.0ml @15:02:28.86 Intake.select :open @15:02:31.05 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:02:31.07 SS.reconfigure SSinhale.with maxSpeed: 48 @15:02:31.11 SS.seek 25ml @15:03:08.56 Stroke completed -- waiting for pressure to equalize ... @15:03:11.17 Intake.select :close @15:03:11.33 SSV.dial :exhaust @15:03:12.09 Exhaust.select :open @15:03:12.20 SS.reconfigure SSexhale @15:03:12.25 SS.seek 0ml @15:03:21.48 Exhaust.select :close @15:03:21.60 SSV.dial CBV @15:03:21.63 CBV.dial SSV @15:03:22.42 Allowing 130s for 25.0ml of remaining 1375.0ml @15:03:22.43 Intake.select :open @15:03:24.64 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:03:24.66 SS.reconfigure SSinhale.with maxSpeed: 48 @15:03:24.71 SS.seek 25ml @15:04:01.41 Stroke completed -- waiting for pressure to equalize ... @15:04:04.03 Intake.select :close @15:04:04.19 SSV.dial :exhaust @15:04:04.95 Exhaust.select :open @15:04:05.06 SS.reconfigure SSexhale @15:04:05.11 SS.seek 0ml @15:04:13.86 Exhaust.select :close @15:04:13.98 SSV.dial CBV @15:04:14.01 CBV.dial SSV @15:04:14.81 Allowing 130s for 25.0ml of remaining 1350.0ml @15:04:14.82 Intake.select :open @15:04:17.00 Intake @32.3psia, vacuum from 20.0 to 24.0psi @15:04:17.02 SS.reconfigure SSinhale.with maxSpeed: 48 @15:04:17.07 SS.seek 25ml @15:04:25.19 GoodNews.email "At 14:00:23 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tundeployv2.rb:110\n{1 => 6, 2 => 16}\nCan@14:00:23, 14.6C, 67% humidity, 14.3psia, 14.699V, 0.682A, 0.676A avg, 10.03W",Subject: "Started 18tundeployv2 phase coreNEG" @15:05:05.30 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:05:05.34 Retry #7 of 10 after 10:00 @15:05:05.38 delay 5 seconds @15:05:05.41 Stroke completed -- waiting for pressure to equalize ... @15:05:06.11 Intake.select :close @15:05:06.26 SSV.dial :exhaust @15:05:07.02 Exhaust.select :open @15:05:07.13 SS.reconfigure SSexhale @15:05:07.18 SS.seek 0ml @15:05:10.41 ATV.dial :air2 @15:05:11.43 AS.seek 0.52ml @15:05:16.17 Exhaust.select :close @15:05:16.29 SSV.dial CBV @15:05:16.32 CBV.dial SSV @15:05:17.67 Allowing 130s for 25.0ml of remaining 1325.0ml @15:05:17.68 Intake.select :open @15:05:19.87 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:05:19.89 SS.reconfigure SSinhale.with maxSpeed: 48 @15:05:19.93 SS.seek 25ml @15:05:57.17 Stroke completed -- waiting for pressure to equalize ... @15:05:59.77 Intake.select :close @15:05:59.94 SSV.dial :exhaust @15:06:00.70 Exhaust.select :open @15:06:00.81 SS.reconfigure SSexhale @15:06:00.87 SS.seek 0ml @15:06:10.05 Exhaust.select :close @15:06:10.17 SSV.dial CBV @15:06:10.20 CBV.dial SSV @15:06:10.98 Allowing 130s for 25.0ml of remaining 1300.0ml @15:06:10.99 Intake.select :open @15:06:13.18 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:06:13.20 SS.reconfigure SSinhale.with maxSpeed: 48 @15:06:13.24 SS.seek 25ml @15:06:49.92 Stroke completed -- waiting for pressure to equalize ... @15:06:52.30 delay 5 seconds @15:06:52.53 Intake.select :close @15:06:52.69 SSV.dial :exhaust @15:06:53.45 Exhaust.select :open @15:06:53.56 SS.reconfigure SSexhale @15:06:53.61 SS.seek 0ml @15:06:57.32 ATV.dial ASV @15:06:58.24 ASV.dial :wasteR @15:06:58.61 AS.seek 0.3ml @15:07:02.70 Exhaust.select :close @15:07:02.82 SSV.dial CBV @15:07:02.85 CBV.dial SSV @15:07:03.63 Allowing 130s for 25.0ml of remaining 1275.0ml @15:07:03.64 Intake.select :open @15:07:05.83 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:07:05.85 SS.reconfigure SSinhale.with maxSpeed: 48 @15:07:05.89 SS.seek 25ml @15:07:43.03 Stroke completed -- waiting for pressure to equalize ... @15:07:43.12 delay 20 seconds @15:07:46.26 Intake.select :close @15:07:46.42 SSV.dial :exhaust @15:07:47.18 Exhaust.select :open @15:07:47.29 SS.reconfigure SSexhale @15:07:47.34 SS.seek 0ml @15:07:56.34 Exhaust.select :close @15:07:56.46 SSV.dial CBV @15:07:56.49 CBV.dial SSV @15:07:57.27 Allowing 130s for 25.0ml of remaining 1250.0ml @15:07:57.28 Intake.select :open @15:07:59.47 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:07:59.49 SS.reconfigure SSinhale.with maxSpeed: 48 @15:07:59.53 SS.seek 25ml @15:08:03.14 AS.seek 0.07ml @15:08:36.70 Stroke completed -- waiting for pressure to equalize ... @15:08:39.94 Intake.select :close @15:08:40.09 SSV.dial :exhaust @15:08:40.85 Exhaust.select :open @15:08:40.96 SS.reconfigure SSexhale @15:08:41.57 SS.seek 0ml @15:08:49.65 delay 20 seconds @15:08:50.55 Exhaust.select :close @15:08:50.67 SSV.dial CBV @15:08:50.70 CBV.dial SSV @15:08:51.49 Allowing 130s for 25.0ml of remaining 1225.0ml @15:08:51.50 Intake.select :open @15:08:53.69 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:08:53.71 SS.reconfigure SSinhale.with maxSpeed: 48 @15:08:53.76 SS.seek 25ml @15:09:09.67 ATV.dial :waste2 @15:09:10.39 AS.reconfigure ASconfig @15:09:10.44 AS.seek :empty @15:09:14.82 Elution @15:09:14.83 ATV.dial :air1 @15:09:15.88 AS.reconfigure ASslow1 @15:09:15.93 AS.seek 0.049ml @15:09:26.31 delay 3 seconds @15:09:29.33 ATV.dial :ElutionWater @15:09:30.08 AS.seek 0.109ml @15:09:30.91 Stroke completed -- waiting for pressure to equalize ... @15:09:34.16 Intake.select :close @15:09:34.32 SSV.dial :exhaust @15:09:35.09 Exhaust.select :open @15:09:35.20 SS.reconfigure SSexhale @15:09:35.25 SS.seek 0ml @15:09:42.42 delay 3 seconds @15:09:44.03 Exhaust.select :close @15:09:44.15 SSV.dial CBV @15:09:44.20 CBV.dial SSV @15:09:44.96 Allowing 130s for 25.0ml of remaining 1200.0ml @15:09:44.97 Intake.select :open @15:09:45.44 ATV.dial ASV,via: :air2 @15:09:47.15 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:09:47.17 SS.reconfigure SSinhale.with maxSpeed: 48 @15:09:47.22 SS.seek 25ml @15:09:47.46 ASV.dial :SPEelution @15:09:48.11 AFV.select :carrierSolution @15:09:48.27 AS.reconfigure ASnorm @15:09:48.32 AS.seek 0.759ml @15:10:19.60 Vacuum too high -- waiting up to 40s @15:10:20.71 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:10:20.73 SS.reconfigure SSinhale.with maxSpeed: 43 @15:10:20.78 SS.seek 25ml @15:10:21.37 delay 3 seconds @15:10:24.38 AFV.select :holdingCoil @15:10:24.54 AS.reconfigure ASslow1 @15:10:24.59 AS.seek 0.474ml @15:10:26.65 Stroke completed -- waiting for pressure to equalize ... @15:10:29.89 Intake.select :close @15:10:30.04 SSV.dial :exhaust @15:10:30.81 Exhaust.select :open @15:10:30.92 SS.reconfigure SSexhale @15:10:30.97 SS.seek 0ml @15:10:39.80 Exhaust.select :close @15:10:39.92 SSV.dial CBV @15:10:39.95 CBV.dial SSV @15:10:40.74 Allowing 130s for 25.0ml of remaining 1175.0ml @15:10:40.75 Intake.select :open @15:10:42.93 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:10:42.95 SS.reconfigure SSinhale.with maxSpeed: 43 @15:10:43.00 SS.seek 25ml @15:11:22.20 delay 5 seconds @15:11:24.75 Stroke completed -- waiting for pressure to equalize ... @15:11:27.28 AS.reconfigure ASslow2 @15:11:27.33 AS.seek 0.439ml @15:11:27.90 Intake.select :close @15:11:28.06 SSV.dial :exhaust @15:11:28.82 Exhaust.select :open @15:11:28.93 SS.reconfigure SSexhale @15:11:28.98 SS.seek 0ml @15:11:37.98 Exhaust.select :close @15:11:38.10 SSV.dial CBV @15:11:38.13 CBV.dial SSV @15:11:38.92 Allowing 130s for 25.0ml of remaining 1150.0ml @15:11:38.93 Intake.select :open @15:11:41.12 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:11:41.14 SS.reconfigure SSinhale.with maxSpeed: 43 @15:11:41.19 SS.seek 25ml @15:12:02.87 delay 1:00 @15:12:22.55 Stroke completed -- waiting for pressure to equalize ... @15:12:26.41 Intake.select :close @15:12:26.57 SSV.dial :exhaust @15:12:27.34 Exhaust.select :open @15:12:27.45 SS.reconfigure SSexhale @15:12:27.50 SS.seek 0ml @15:12:36.58 Exhaust.select :close @15:12:36.70 SSV.dial CBV @15:12:36.73 CBV.dial SSV @15:12:37.52 Allowing 130s for 25.0ml of remaining 1125.0ml @15:12:37.53 Intake.select :open @15:12:39.73 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:12:39.75 SS.reconfigure SSinhale.with maxSpeed: 43 @15:12:39.80 SS.seek 25ml @15:13:02.89 pushing eluate to holding coil @15:13:02.96 AS.seek 0.279ml @15:13:21.08 Stroke completed -- waiting for pressure to equalize ... @15:13:24.32 Intake.select :close @15:13:24.48 SSV.dial :exhaust @15:13:25.23 Exhaust.select :open @15:13:25.35 SS.reconfigure SSexhale @15:13:25.40 SS.seek 0ml @15:13:34.72 Exhaust.select :close @15:13:34.84 SSV.dial CBV @15:13:34.87 CBV.dial SSV @15:13:35.65 Allowing 130s for 25.0ml of remaining 1100.0ml @15:13:35.66 Intake.select :open @15:13:37.85 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:13:37.87 SS.reconfigure SSinhale.with maxSpeed: 43 @15:13:37.91 SS.seek 25ml @15:14:18.72 Stroke completed -- waiting for pressure to equalize ... @15:14:21.96 Intake.select :close @15:14:22.13 SSV.dial :exhaust @15:14:22.87 Exhaust.select :open @15:14:22.99 SS.reconfigure SSexhale @15:14:23.04 SS.seek 0ml @15:14:32.11 Exhaust.select :close @15:14:32.23 SSV.dial CBV @15:14:32.26 CBV.dial SSV @15:14:33.09 Allowing 130s for 25.0ml of remaining 1075.0ml @15:14:33.10 Intake.select :open @15:14:35.85 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:14:35.87 SS.reconfigure SSinhale.with maxSpeed: 43 @15:14:35.92 SS.seek 25ml @15:15:05.38 GoodNews.email "At 14:00:23 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tundeployv2.rb:110\n{1 => 6, 2 => 16}\nCan@14:00:23, 14.6C, 67% humidity, 14.3psia, 14.699V, 0.682A, 0.676A avg, 10.03W",Subject: "Started 18tundeployv2 phase coreNEG" @15:15:45.52 Stroke completed -- waiting for pressure to equalize ... @15:15:45.55 delay 30 seconds @15:15:45.57 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:15:45.59 Retry #8 of 10 after 10:00 @15:15:46.24 Intake.select :close @15:15:46.40 SSV.dial :exhaust @15:15:47.16 Exhaust.select :open @15:15:47.27 SS.reconfigure SSexhale @15:15:47.32 SS.seek 0ml @15:15:56.47 Exhaust.select :close @15:15:56.59 SSV.dial CBV @15:15:56.62 CBV.dial SSV @15:15:57.41 Allowing 130s for 25.0ml of remaining 1050.0ml @15:15:57.42 Intake.select :open @15:15:59.61 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:15:59.63 SS.reconfigure SSinhale.with maxSpeed: 43 @15:15:59.68 SS.seek 25ml @15:16:15.58 ATV.dial :waste,via: :air3 @15:16:17.17 AS.reconfigure ASconfig @15:16:17.22 AS.seek :empty @15:16:33.62 Parking Bleach in LysateSource line and SPEcolumn @15:16:33.63 ATV.dial :air3 @15:16:34.52 AS.reconfigure ASslow1 @15:16:34.57 AS.seek 0.029ml @15:16:40.90 ATV.dial :ElutionWater @15:16:41.05 Stroke completed -- waiting for pressure to equalize ... @15:16:41.49 AS.seek 0.059ml @15:16:44.95 Intake.select :close @15:16:45.12 SSV.dial :exhaust @15:16:45.88 Exhaust.select :open @15:16:46.00 SS.reconfigure SSexhale @15:16:46.05 SS.seek 0ml @15:16:47.80 ATV.dial :air3 @15:16:48.40 AS.seek 0.069ml @15:16:50.67 delay 2 seconds @15:16:52.68 ATV.dial :bleach @15:16:53.13 AS.seek 0.769ml @15:16:54.90 Exhaust.select :close @15:16:55.02 SSV.dial CBV @15:16:55.05 CBV.dial SSV @15:16:55.84 Allowing 130s for 25.0ml of remaining 1025.0ml @15:16:55.85 Intake.select :open @15:16:58.10 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:16:58.13 SS.reconfigure SSinhale.with maxSpeed: 43 @15:16:58.18 SS.seek 25ml @15:17:25.82 Vacuum too high -- waiting up to 40s @15:17:26.67 Intake @32.9psia, vacuum from 20.0 to 24.0psi @15:17:26.69 SS.reconfigure SSinhale.with maxSpeed: 39 @15:17:26.74 SS.seek 25ml @15:17:42.37 Stroke completed -- waiting for pressure to equalize ... @15:17:45.62 Intake.select :close @15:17:45.78 SSV.dial :exhaust @15:17:46.54 Exhaust.select :open @15:17:46.65 SS.reconfigure SSexhale @15:17:46.70 SS.seek 0ml @15:17:55.97 Exhaust.select :close @15:17:56.09 SSV.dial CBV @15:17:56.12 CBV.dial SSV @15:17:57.55 Allowing 130s for 25.0ml of remaining 1000.0ml @15:17:57.56 Intake.select :open @15:17:59.75 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:17:59.77 SS.reconfigure SSinhale.with maxSpeed: 39 @15:17:59.82 SS.seek 25ml @15:18:44.69 Stroke completed -- waiting for pressure to equalize ... @15:18:48.56 Intake.select :close @15:18:48.73 SSV.dial :exhaust @15:18:49.49 Exhaust.select :open @15:18:49.60 SS.reconfigure SSexhale @15:18:49.65 SS.seek 0ml @15:18:58.98 Exhaust.select :close @15:18:59.10 SSV.dial CBV @15:18:59.13 CBV.dial SSV @15:18:59.91 Allowing 130s for 25.0ml of remaining 975.0ml @15:18:59.92 Intake.select :open @15:19:02.10 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:19:02.12 SS.reconfigure SSinhale.with maxSpeed: 39 @15:19:02.17 SS.seek 25ml @15:19:14.19 delay 3 seconds @15:19:17.21 ATV.dial :air3 @15:19:17.73 delay 3 seconds @15:19:20.82 AS.seek 0.794ml @15:19:26.12 delay 3 seconds @15:19:29.14 ATV.dial :bleach @15:19:29.74 AS.seek 0.779ml @15:19:33.05 delay 3 seconds @15:19:36.07 ATV.dial :AIV @15:19:37.33 AS.reconfigure ASconfig @15:19:37.38 AS.seek 0.015ml @15:19:47.50 Stroke completed -- waiting for pressure to equalize ... @15:19:51.37 Intake.select :close @15:19:51.52 SSV.dial :exhaust @15:19:52.28 Exhaust.select :open @15:19:52.40 SS.reconfigure SSexhale @15:19:52.45 SS.seek 0ml @15:20:01.25 Exhaust.select :close @15:20:01.37 SSV.dial CBV @15:20:01.40 CBV.dial SSV @15:20:02.20 Allowing 130s for 25.0ml of remaining 950.0ml @15:20:02.21 Intake.select :open @15:20:04.39 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:20:04.41 SS.reconfigure SSinhale.with maxSpeed: 39 @15:20:04.46 SS.seek 25ml @15:20:21.58 ATV.dial :waste @15:20:22.97 AS.seek :empty @15:20:24.20 ATV.dial :air3 @15:20:24.85 AS.reconfigure ASslow1 @15:20:24.90 AS.seek 0.009ml @15:20:27.19 delay 2 seconds @15:20:29.20 ATV.dial :bleach @15:20:29.76 AS.seek 0.579ml @15:20:50.26 Stroke completed -- waiting for pressure to equalize ... @15:20:54.76 Intake.select :close @15:20:54.91 SSV.dial :exhaust @15:20:56.24 Exhaust.select :open @15:20:56.35 SS.reconfigure SSexhale @15:20:56.41 SS.seek 0ml @15:21:05.42 Exhaust.select :close @15:21:05.60 SSV.dial CBV @15:21:05.64 CBV.dial SSV @15:21:06.43 Allowing 130s for 25.0ml of remaining 925.0ml @15:21:06.45 Intake.select :open @15:21:08.63 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:21:08.65 SS.reconfigure SSinhale.with maxSpeed: 39 @15:21:08.72 SS.seek 25ml @15:21:54.14 Stroke completed -- waiting for pressure to equalize ... @15:21:58.78 Intake.select :close @15:21:58.94 SSV.dial :exhaust @15:21:59.71 Exhaust.select :open @15:21:59.83 SS.reconfigure SSexhale @15:21:59.88 SS.seek 0ml @15:22:09.17 Exhaust.select :close @15:22:09.29 SSV.dial CBV @15:22:09.32 CBV.dial SSV @15:22:10.12 Allowing 130s for 25.0ml of remaining 900.0ml @15:22:10.13 Intake.select :open @15:22:12.31 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:22:12.33 SS.reconfigure SSinhale.with maxSpeed: 39 @15:22:12.38 SS.seek 25ml @15:22:24.58 delay 3 seconds @15:22:27.61 ATV.dial :air3 @15:22:28.13 delay 3 seconds @15:22:31.22 AS.seek 0.604ml @15:22:36.56 delay 3 seconds @15:22:39.03 Vacuum too high -- waiting up to 40s @15:22:39.58 ATV.dial :bleach @15:22:39.89 Intake @32.6psia, vacuum from 20.0 to 24.0psi @15:22:39.91 SS.reconfigure SSinhale.with maxSpeed: 35 @15:22:39.96 SS.seek 25ml @15:22:40.18 AS.seek 0.589ml @15:22:43.47 delay 3 seconds @15:22:46.48 ATV.dial :ASV @15:22:47.45 ASV.dial :wasteR @15:22:48.08 AS.seek 0ml @15:23:00.89 Stroke completed -- waiting for pressure to equalize ... @15:23:04.77 Intake.select :close @15:23:04.93 SSV.dial :exhaust @15:23:05.69 Exhaust.select :open @15:23:05.81 SS.reconfigure SSexhale @15:23:05.86 SS.seek 0ml @15:23:14.81 Exhaust.select :close @15:23:14.93 SSV.dial CBV @15:23:14.96 CBV.dial SSV @15:23:15.76 Allowing 130s for 25.0ml of remaining 875.0ml @15:23:15.77 Intake.select :open @15:23:17.95 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:23:17.97 SS.reconfigure SSinhale.with maxSpeed: 35 @15:23:18.02 SS.seek 25ml @15:24:08.25 Stroke completed -- waiting for pressure to equalize ... @15:24:12.68 Intake.select :close @15:24:12.84 SSV.dial :exhaust @15:24:13.61 Exhaust.select :open @15:24:13.72 SS.reconfigure SSexhale @15:24:13.77 SS.seek 0ml @15:24:22.86 Exhaust.select :close @15:24:22.98 SSV.dial CBV @15:24:23.01 CBV.dial SSV @15:24:23.79 Allowing 130s for 25.0ml of remaining 850.0ml @15:24:23.80 Intake.select :open @15:24:25.99 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:24:26.01 SS.reconfigure SSinhale.with maxSpeed: 35 @15:24:26.06 SS.seek 25ml @15:24:46.83 delay 1:00 @15:25:16.51 Stroke completed -- waiting for pressure to equalize ... @15:25:21.04 Intake.select :close @15:25:21.20 SSV.dial :exhaust @15:25:21.97 Exhaust.select :open @15:25:22.08 SS.reconfigure SSexhale @15:25:22.13 SS.seek 0ml @15:25:31.04 Exhaust.select :close @15:25:31.16 SSV.dial CBV @15:25:31.19 CBV.dial SSV @15:25:31.98 Allowing 130s for 25.0ml of remaining 825.0ml @15:25:31.99 Intake.select :open @15:25:34.18 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:25:34.20 SS.reconfigure SSinhale.with maxSpeed: 35 @15:25:34.24 SS.seek 25ml @15:25:45.62 GoodNews.email "At 14:00:23 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tundeployv2.rb:110\n{1 => 6, 2 => 16}\nCan@14:00:23, 14.6C, 67% humidity, 14.3psia, 14.699V, 0.682A, 0.676A avg, 10.03W",Subject: "Started 18tundeployv2 phase coreNEG" @15:26:25.74 Stroke completed -- waiting for pressure to equalize ... @15:26:25.77 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:26:25.79 Retry #9 of 10 after 10:00 @15:26:25.81 AFV.select :carrierSolution @15:26:25.92 delay 2 seconds @15:26:27.94 AS.reconfigure ASconfig @15:26:27.99 AS.seek 1ml @15:26:29.63 Intake.select :close @15:26:29.79 SSV.dial :exhaust @15:26:30.55 Exhaust.select :open @15:26:30.66 SS.reconfigure SSexhale @15:26:30.71 SS.seek 0ml @15:26:39.56 Exhaust.select :close @15:26:39.68 SSV.dial CBV @15:26:39.71 CBV.dial SSV @15:26:40.51 Allowing 130s for 25.0ml of remaining 800.0ml @15:26:40.52 Intake.select :open @15:26:42.70 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:26:42.72 SS.reconfigure SSinhale.with maxSpeed: 35 @15:26:42.77 SS.seek 25ml @15:27:25.77 delay 1 second @15:27:26.78 AFV.select :holdingCoil @15:27:26.89 delay 1 second @15:27:27.91 ATV.dial ASV @15:27:27.93 ASV.dial :wasteR @15:27:27.95 AS.reconfigure ASslow1 @15:27:27.99 AS.seek 0ml @15:27:33.54 Stroke completed -- waiting for pressure to equalize ... @15:27:38.70 Intake.select :close @15:27:39.50 SSV.dial :exhaust @15:27:40.26 Exhaust.select :open @15:27:40.37 SS.reconfigure SSexhale @15:27:40.43 SS.seek 0ml @15:27:49.12 Exhaust.select :close @15:27:49.24 SSV.dial CBV @15:27:49.27 CBV.dial SSV @15:27:50.07 Allowing 130s for 25.0ml of remaining 775.0ml @15:27:50.08 Intake.select :open @15:27:52.27 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:27:52.29 SS.reconfigure SSinhale.with maxSpeed: 35 @15:27:52.34 SS.seek 25ml @15:28:42.13 Vacuum too high -- waiting up to 40s @15:28:43.76 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:28:43.78 SS.reconfigure SSinhale.with maxSpeed: 32 @15:28:43.83 SS.seek 25ml @15:28:45.01 Stroke completed -- waiting for pressure to equalize ... @15:28:48.89 Intake.select :close @15:28:49.05 SSV.dial :exhaust @15:28:49.80 Exhaust.select :open @15:28:49.91 SS.reconfigure SSexhale @15:28:49.97 SS.seek 0ml @15:28:59.06 Exhaust.select :close @15:28:59.18 SSV.dial CBV @15:28:59.21 CBV.dial SSV @15:28:59.99 Allowing 130s for 25.0ml of remaining 750.0ml @15:29:00.00 Intake.select :open @15:29:02.19 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:29:02.21 SS.reconfigure SSinhale.with maxSpeed: 32 @15:29:02.26 SS.seek 25ml @15:29:57.31 Stroke completed -- waiting for pressure to equalize ... @15:30:02.46 Intake.select :close @15:30:02.62 SSV.dial :exhaust @15:30:03.38 Exhaust.select :open @15:30:03.49 SS.reconfigure SSexhale @15:30:03.54 SS.seek 0ml @15:30:12.72 Exhaust.select :close @15:30:12.84 SSV.dial CBV @15:30:12.87 CBV.dial SSV @15:30:13.66 Allowing 130s for 25.0ml of remaining 725.0ml @15:30:13.67 Intake.select :open @15:30:15.86 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:30:15.88 SS.reconfigure SSinhale.with maxSpeed: 32 @15:30:15.92 SS.seek 25ml @15:30:49.39 delay 20 seconds @15:31:09.41 ATV.dial :air3 @15:31:10.75 Stroke completed -- waiting for pressure to equalize ... @15:31:10.86 AS.seek 0.59ml @15:31:15.88 Intake.select :close @15:31:16.04 SSV.dial :exhaust @15:31:16.80 Exhaust.select :open @15:31:16.91 SS.reconfigure SSexhale @15:31:16.96 SS.seek 0ml @15:31:26.04 Exhaust.select :close @15:31:26.16 SSV.dial CBV @15:31:26.19 CBV.dial SSV @15:31:26.98 Allowing 130s for 25.0ml of remaining 700.0ml @15:31:26.99 Intake.select :open @15:31:29.17 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:31:29.20 SS.reconfigure SSinhale.with maxSpeed: 32 @15:31:29.24 SS.seek 25ml @15:32:24.29 Stroke completed -- waiting for pressure to equalize ... @15:32:30.09 Intake.select :close @15:32:30.26 SSV.dial :exhaust @15:32:31.07 Exhaust.select :open @15:32:31.18 SS.reconfigure SSexhale @15:32:31.23 SS.seek 0ml @15:32:40.11 Exhaust.select :close @15:32:40.23 SSV.dial CBV @15:32:40.26 CBV.dial SSV @15:32:41.04 Allowing 130s for 25.0ml of remaining 675.0ml @15:32:41.05 Intake.select :open @15:32:43.23 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:32:43.25 SS.reconfigure SSinhale.with maxSpeed: 32 @15:32:43.30 SS.seek 25ml @15:33:09.78 delay 3 seconds @15:33:12.80 ATV.dial :ASV @15:33:14.21 ASV.dial :wasteR @15:33:14.29 AS.seek 0.09ml @15:33:20.69 Vacuum too high -- waiting up to 40s @15:33:22.30 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:33:22.32 SS.reconfigure SSinhale.with maxSpeed: 29 @15:33:22.37 SS.seek 25ml @15:33:42.54 Stroke completed -- waiting for pressure to equalize ... @15:33:47.68 Intake.select :close @15:33:47.84 SSV.dial :exhaust @15:33:48.60 Exhaust.select :open @15:33:48.71 SS.reconfigure SSexhale @15:33:48.76 SS.seek 0ml @15:33:57.96 Exhaust.select :close @15:33:58.07 SSV.dial CBV @15:33:58.10 CBV.dial SSV @15:33:58.90 Allowing 130s for 25.0ml of remaining 650.0ml @15:33:58.91 Intake.select :open @15:34:01.09 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:34:01.65 SS.reconfigure SSinhale.with maxSpeed: 29 @15:34:01.72 SS.seek 25ml @15:34:55.11 delay 30 seconds @15:35:02.15 Stroke completed -- waiting for pressure to equalize ... @15:35:07.30 Intake.select :close @15:35:07.46 SSV.dial :exhaust @15:35:08.23 Exhaust.select :open @15:35:08.34 SS.reconfigure SSexhale @15:35:08.39 SS.seek 0ml @15:35:17.25 Exhaust.select :close @15:35:17.37 SSV.dial CBV @15:35:17.40 CBV.dial SSV @15:35:18.19 Allowing 130s for 25.0ml of remaining 625.0ml @15:35:18.20 Intake.select :open @15:35:20.39 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:35:20.41 SS.reconfigure SSinhale.with maxSpeed: 29 @15:35:20.45 SS.seek 25ml @15:35:25.12 ATV.dial :extSrc @15:35:25.94 delay 3 seconds @15:35:28.96 ATV.dial :ElutionWater @15:35:30.01 AS.seek 0.12ml @15:35:36.32 ATV.dial :air3 @15:35:36.90 AS.seek 0.13ml @15:35:39.18 delay 2 seconds @15:35:41.19 ATV.dial :bleach @15:35:41.65 AS.seek 0.23ml @15:36:02.03 delay 3 seconds @15:36:05.05 ATV.dial :air3 @15:36:05.58 delay 3 seconds @15:36:08.66 AS.seek 0.255ml @15:36:13.99 delay 3 seconds @15:36:17.01 ATV.dial :bleach @15:36:17.61 AS.seek 0.24ml @15:36:20.93 delay 3 seconds @15:36:21.14 Stroke completed -- waiting for pressure to equalize ... @15:36:23.94 ATV.dial :ASV @15:36:24.93 ASV.dial :SPEdelivery @15:36:25.35 AFV.select :carrierSolution @15:36:25.47 delay 2 seconds @15:36:25.81 GoodNews.email "At 14:00:23 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tundeployv2.rb:110\n{1 => 6, 2 => 16}\nCan@14:00:23, 14.6C, 67% humidity, 14.3psia, 14.699V, 0.682A, 0.676A avg, 10.03W",Subject: "Started 18tundeployv2 phase coreNEG" @15:37:05.92 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:37:05.95 Retry #10 of 10 after 10:00 @15:37:06.08 AS.reconfigure ASconfig @15:37:06.16 AS.seek 0.5ml @15:37:06.27 Intake.select :close @15:37:06.43 SSV.dial :exhaust @15:37:07.19 Exhaust.select :open @15:37:07.30 SS.reconfigure SSexhale @15:37:07.35 SS.seek 0ml @15:37:16.29 Exhaust.select :close @15:37:16.41 SSV.dial CBV @15:37:16.44 CBV.dial SSV @15:37:17.24 Allowing 130s for 25.0ml of remaining 600.0ml @15:37:17.25 Intake.select :open @15:37:19.43 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:37:19.45 SS.reconfigure SSinhale.with maxSpeed: 29 @15:37:19.50 SS.seek 25ml @15:37:21.45 delay 1 second @15:37:22.47 AFV.select :holdingCoil @15:37:22.63 AS.reconfigure ASslow1 @15:37:22.68 AS.seek 0.17ml @15:38:20.09 Stroke completed -- waiting for pressure to equalize ... @15:38:27.09 Intake.select :close @15:38:27.26 SSV.dial :exhaust @15:38:28.04 Exhaust.select :open @15:38:28.15 SS.reconfigure SSexhale @15:38:28.20 SS.seek 0ml @15:38:29.36 SPE.hold 2:46:40,95C @15:38:29.43 delay 3:00 @15:38:37.04 Exhaust.select :close @15:38:37.16 SSV.dial CBV @15:38:37.19 CBV.dial SSV @15:38:37.98 Allowing 130s for 25.0ml of remaining 575.0ml @15:38:37.99 Intake.select :open @15:38:40.18 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:38:40.20 SS.reconfigure SSinhale.with maxSpeed: 29 @15:38:40.25 SS.seek 25ml @15:39:31.38 Vacuum too high -- waiting up to 40s @15:39:33.26 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:39:33.28 SS.reconfigure SSinhale.with maxSpeed: 26 @15:39:33.33 SS.seek 25ml @15:39:44.46 Stroke completed -- waiting for pressure to equalize ... @15:39:50.26 Intake.select :close @15:39:50.42 SSV.dial :exhaust @15:39:51.19 Exhaust.select :open @15:39:51.30 SS.reconfigure SSexhale @15:39:51.35 SS.seek 0ml @15:40:00.65 Exhaust.select :close @15:40:00.77 SSV.dial CBV @15:40:00.80 CBV.dial SSV @15:40:01.60 Allowing 130s for 25.0ml of remaining 550.0ml @15:40:01.61 Intake.select :open @15:40:03.79 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:40:03.81 SS.reconfigure SSinhale.with maxSpeed: 26 @15:40:03.86 SS.seek 25ml @15:41:11.37 Stroke completed -- waiting for pressure to equalize ... @15:41:18.40 Intake.select :close @15:41:18.56 SSV.dial :exhaust @15:41:19.33 Exhaust.select :open @15:41:19.44 SS.reconfigure SSexhale @15:41:19.49 SS.seek 0ml @15:41:28.43 Exhaust.select :close @15:41:28.55 SSV.dial CBV @15:41:28.58 CBV.dial SSV @15:41:29.38 Allowing 130s for 25.0ml of remaining 525.0ml @15:41:29.39 Intake.select :open @15:41:29.46 AFV.select :carrierSolution @15:41:29.57 delay 2 seconds @15:41:31.59 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:41:31.61 SS.reconfigure SSinhale.with maxSpeed: 26 @15:41:31.66 SS.seek 25ml @15:41:31.72 AS.reconfigure ASconfig @15:41:31.76 AS.seek 1ml @15:42:19.77 delay 1 second @15:42:20.78 AFV.select :holdingCoil @15:42:20.89 delay 1 second @15:42:21.91 ASV.dial :wasteD @15:42:22.86 AS.seek 0ml @15:42:39.43 Stroke completed -- waiting for pressure to equalize ... @15:42:46.56 Intake.select :close @15:42:46.72 SSV.dial :exhaust @15:42:47.49 Exhaust.select :open @15:42:47.60 SS.reconfigure SSexhale @15:42:47.65 SS.seek 0ml @15:42:56.75 Exhaust.select :close @15:42:56.87 SSV.dial CBV @15:42:56.90 CBV.dial SSV @15:42:57.69 Allowing 130s for 25.0ml of remaining 500.0ml @15:42:57.70 Intake.select :open @15:42:59.90 Intake @34.8psia, vacuum from 20.0 to 24.0psi @15:42:59.92 SS.reconfigure SSinhale.with maxSpeed: 26 @15:42:59.97 SS.seek 25ml @15:43:20.65 Priming Eluate @15:43:20.66 AFV.select :carrierSolution @15:43:20.77 delay 2 seconds @15:43:22.80 AS.seek 0.5ml @15:43:35.56 Vacuum too high -- waiting up to 40s @15:43:37.20 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:43:37.22 SS.reconfigure SSinhale.with maxSpeed: 23 @15:43:37.27 SS.seek 25ml @15:43:51.86 delay 1 second @15:43:52.87 AFV.select :holdingCoil @15:43:52.98 ATV.dial ASV @15:43:52.99 ASV.dial :SPErecovery @15:43:53.55 Swish #1 of 2 @15:43:53.63 AS.reconfigure ASslow0 @15:43:53.68 AS.seek 0.6ml @15:44:04.14 AS.seek 0.5ml @15:44:13.45 Stroke completed -- waiting for pressure to equalize ... @15:44:14.53 Swish #2 of 2 @15:44:14.61 AS.seek 0.6ml @15:44:19.88 Intake.select :close @15:44:20.04 SSV.dial :exhaust @15:44:20.82 Exhaust.select :open @15:44:20.93 SS.reconfigure SSexhale @15:44:20.99 SS.seek 0ml @15:44:25.11 AS.seek 0.5ml @15:44:30.47 Exhaust.select :close @15:44:30.59 SSV.dial CBV @15:44:30.62 CBV.dial SSV @15:44:31.40 Allowing 130s for 25.0ml of remaining 475.0ml @15:44:31.41 Intake.select :open @15:44:33.60 Intake @35.2psia, vacuum from 20.0 to 24.0psi @15:44:33.62 SS.reconfigure SSinhale.with maxSpeed: 23 @15:44:33.67 SS.seek 25ml @15:44:35.53 delay 3 seconds @15:44:38.64 AS.reconfigure ASslow1 @15:44:38.69 AS.seek 0.684ml @15:45:15.99 delay 3 seconds @15:45:19.02 Eluate primed @15:45:19.03 ATV.dial :waste2 @15:45:19.69 AS.reconfigure ASnorm @15:45:19.74 AS.seek 0ml @15:45:49.92 Stroke completed -- waiting for pressure to equalize ... @15:45:54.54 SPE.coast @15:45:54.63 ASV.dial :SPErecovery @15:45:54.65 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0215h.pcr @15:45:54.66 Building PCR Slug from ASV with MM2 mix, PP4 primer @15:45:54.68 Priming with carrier @15:45:54.69 ATV.dial ARV @15:45:55.61 ARV.dial :carrier @15:45:55.97 AS.seek 0.5ml @15:45:56.44 Intake.select :close @15:45:56.61 SSV.dial :exhaust @15:45:57.37 Exhaust.select :open @15:45:57.48 SS.reconfigure SSexhale @15:45:57.54 SS.seek 0ml @15:46:06.53 Exhaust.select :close @15:46:06.65 SSV.dial CBV @15:46:06.68 CBV.dial SSV @15:46:07.47 Allowing 130s for 25.0ml of remaining 450.0ml @15:46:07.48 Intake.select :open @15:46:09.67 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:46:09.69 SS.reconfigure SSinhale.with maxSpeed: 23 @15:46:09.74 SS.seek 25ml @15:46:21.56 AFV.select :carrierSolution @15:46:21.68 delay 2 seconds @15:46:23.71 AS.reconfigure ASconfig @15:46:23.76 AS.seek 1ml @15:46:52.82 delay 1 second @15:46:53.83 AFV.select :holdingCoil @15:46:53.94 delay 1 second @15:46:54.96 ATV.dial :waste @15:46:56.33 AS.reconfigure ASnorm @15:46:56.38 AS.seek :empty @15:47:05.99 GoodNews.email "At 14:00:23 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tundeployv2.rb:110\n{1 => 6, 2 => 16}\nCan@14:00:23, 14.6C, 67% humidity, 14.3psia, 14.699V, 0.682A, 0.676A avg, 10.03W",Subject: "Started 18tundeployv2 phase coreNEG" @15:47:46.11 Stroke completed -- waiting for pressure to equalize ... @15:47:46.14 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:47:46.16 BACfans.email "Started mfb in tube 2 at 14:05:12CEST02-Oct-18\n{1 => 6, 2 => 16}\nCan@14:00:23, 14.6C, 67% humidity, 14.3psia, 14.699V, 0.682A, 0.676A avg, 10.03W",Subject: "pcrBAC wcr sampling at most 1500ml" @15:48:16.24 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:48:16.26 Retry #1 of 10 after 10:00 @15:48:16.29 AFV.select :carrierSolution @15:48:16.48 delay 2 seconds @15:48:16.52 Intake.select :close @15:48:16.66 SSV.dial :exhaust @15:48:17.50 Exhaust.select :open @15:48:17.70 SS.reconfigure SSexhale @15:48:17.79 SS.seek 0ml @15:48:18.61 AS.reconfigure ASconfig @15:48:18.95 AS.seek 0.5ml @15:48:27.03 Exhaust.select :close @15:48:30.34 SSV.dial CBV @15:48:30.38 CBV.dial SSV @15:48:31.16 Allowing 130s for 25.0ml of remaining 425.0ml @15:48:31.17 Intake.select :open @15:48:33.36 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:48:33.38 SS.reconfigure SSinhale.with maxSpeed: 23 @15:48:33.43 SS.seek 25ml @15:48:48.38 delay 1 second @15:48:49.40 AFV.select :holdingCoil @15:48:49.51 Building Slug @15:48:49.52 ATV.dial :ARV @15:48:51.02 delay 2 seconds @15:48:53.04 ARV.dial :air1 @15:48:53.44 delay 2 seconds @15:48:55.53 AS.reconfigure ASslow2 @15:48:55.58 AS.seek 0.506ml @15:49:01.85 delay 2 seconds @15:49:03.87 ARV.dial :MM2 @15:49:05.27 delay 2 seconds @15:49:07.30 Adding MM2 @15:49:07.37 AS.seek 0.524ml @15:49:25.69 delay 25 seconds @15:49:49.57 Stroke completed -- waiting for pressure to equalize ... @15:49:50.70 ARV.dial :PP4 @15:49:51.10 Adding PP4 @15:49:51.11 delay 2 seconds @15:49:53.20 AS.seek 0.53ml @15:49:57.32 Intake.select :close @15:49:57.48 SSV.dial :exhaust @15:49:58.24 Exhaust.select :open @15:49:58.35 SS.reconfigure SSexhale @15:49:58.40 SS.seek 0ml @15:49:59.37 delay 10 seconds @15:50:07.29 Exhaust.select :close @15:50:07.41 SSV.dial CBV @15:50:07.44 CBV.dial SSV @15:50:08.24 Allowing 130s for 25.0ml of remaining 400.0ml @15:50:08.25 Intake.select :open @15:50:09.39 ARV.dial :air3 @15:50:09.79 adding ARV air @15:50:09.80 delay 2 seconds @15:50:10.44 Intake @33.2psia, vacuum from 20.0 to 24.0psi @15:50:10.46 SS.reconfigure SSinhale.with maxSpeed: 23 @15:50:10.51 SS.seek 25ml @15:50:11.89 AS.seek 0.536ml @15:50:18.19 delay 5 seconds @15:50:23.21 ARV.dial :carrier @15:50:24.41 delay 7 seconds @15:50:31.50 AS.seek 0.594ml @15:51:27.03 Stroke completed -- waiting for pressure to equalize ... @15:51:30.26 delay 5 seconds @15:51:35.93 ATV.dial :ASV @15:51:36.00 Intake.select :close @15:51:36.16 SSV.dial :exhaust @15:51:36.30 delay 2 seconds @15:51:36.93 Exhaust.select :open @15:51:37.05 SS.reconfigure SSexhale @15:51:37.12 SS.seek 0ml @15:51:38.40 AS.seek 0.6ml @15:51:44.98 delay 4 seconds @15:51:46.31 Exhaust.select :close @15:51:46.43 SSV.dial CBV @15:51:46.46 CBV.dial SSV @15:51:47.26 Allowing 130s for 25.0ml of remaining 375.0ml @15:51:47.27 Intake.select :open @15:51:49.00 ATV.dial ARV @15:51:49.46 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:51:49.48 SS.reconfigure SSinhale.with maxSpeed: 23 @15:51:49.53 SS.seek 25ml @15:51:49.63 delay 2 seconds @15:51:51.65 ARV.dial :carrier @15:51:51.67 delay 2 seconds @15:51:53.76 AS.seek 0.65ml @15:52:26.09 Vacuum too high -- waiting up to 40s @15:52:27.73 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:52:27.75 SS.reconfigure SSinhale.with maxSpeed: 21 @15:52:27.80 SS.seek 25ml @15:52:44.35 delay 4 seconds @15:52:48.37 ATV.dial PCR @15:52:49.05 ASV.dial :waste1 @15:52:49.80 ARV.dial :air1 @15:52:50.18 Positioning Slug by 0.324ml @15:52:50.27 AS.seek 0.326ml @15:53:11.68 Stroke completed -- waiting for pressure to equalize ... @15:53:20.99 Intake.select :close @15:53:21.15 SSV.dial :exhaust @15:53:21.92 Exhaust.select :open @15:53:22.04 SS.reconfigure SSexhale @15:53:22.10 SS.seek 0ml @15:53:30.90 Exhaust.select :close @15:53:31.02 SSV.dial CBV @15:53:31.05 CBV.dial SSV @15:53:31.83 Allowing 130s for 25.0ml of remaining 350.0ml @15:53:31.84 Intake.select :open @15:53:34.03 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:53:34.05 SS.reconfigure SSinhale.with maxSpeed: 21 @15:53:34.09 SS.seek 25ml @15:54:52.78 Vacuum too high -- waiting up to 40s @15:54:54.77 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:54:54.79 SS.reconfigure SSinhale.with maxSpeed: 19 @15:54:54.84 SS.seek 25ml @15:55:00.74 Stroke completed -- waiting for pressure to equalize ... @15:55:07.84 Intake.select :close @15:55:08.00 SSV.dial :exhaust @15:55:08.77 Exhaust.select :open @15:55:08.88 SS.reconfigure SSexhale @15:55:08.94 SS.seek 0ml @15:55:17.97 Exhaust.select :close @15:55:18.09 SSV.dial CBV @15:55:18.12 CBV.dial SSV @15:55:18.91 Allowing 130s for 25.0ml of remaining 325.0ml @15:55:18.92 Intake.select :open @15:55:21.11 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:55:21.13 SS.reconfigure SSinhale.with maxSpeed: 19 @15:55:21.18 SS.seek 25ml @15:56:53.53 Stroke completed -- waiting for pressure to equalize ... @15:57:01.92 Intake.select :close @15:57:02.09 SSV.dial :exhaust @15:57:02.86 Exhaust.select :open @15:57:02.97 SS.reconfigure SSexhale @15:57:03.02 SS.seek 0ml @15:57:12.14 Exhaust.select :close @15:57:12.26 SSV.dial CBV @15:57:12.29 CBV.dial SSV @15:57:13.07 Allowing 130s for 25.0ml of remaining 300.0ml @15:57:13.08 Intake.select :open @15:57:15.27 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:57:15.29 SS.reconfigure SSinhale.with maxSpeed: 19 @15:57:15.34 SS.seek 25ml @15:58:16.32 delay 5 seconds @15:58:16.34 BACfans.email "Started mfb in tube 2 at 14:05:12CEST02-Oct-18\n{1 => 6, 2 => 16}\nCan@14:00:23, 14.6C, 67% humidity, 14.3psia, 14.699V, 0.682A, 0.676A avg, 10.03W",Subject: "pcrBAC wcr sampling at most 1500ml" @15:58:21.34 ATV.dial ARV @15:58:22.06 ASV.dial :PCRlock @15:58:22.51 delay 5 seconds @15:58:27.54 Running Tt1 Profile60C300 thermocycling @15:58:27.55 PCR.program Profile60C300 @15:58:27.70 Profile60C300 should complete in < 1:45:21 @15:58:27.71 PCR.run 2:16:41.25 @15:58:47.35 Stroke completed -- waiting for pressure to equalize ... @15:58:57.04 Intake.select :close @15:58:57.20 SSV.dial :exhaust @15:58:57.97 Exhaust.select :open @15:58:58.09 SS.reconfigure SSexhale @15:58:58.15 SS.seek 0ml @15:59:07.09 Exhaust.select :close @15:59:07.22 SSV.dial CBV @15:59:07.25 CBV.dial SSV @15:59:08.04 Allowing 130s for 25.0ml of remaining 275.0ml @15:59:08.05 Intake.select :open @15:59:10.24 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:59:10.26 SS.reconfigure SSinhale.with maxSpeed: 19 @15:59:10.30 SS.seek 25ml @16:00:42.68 Stroke completed -- waiting for pressure to equalize ... @16:00:52.96 Intake.select :close @16:00:53.12 SSV.dial :exhaust @16:00:53.88 Exhaust.select :open @16:00:53.99 SS.reconfigure SSexhale @16:00:54.04 SS.seek 0ml @16:01:03.73 Exhaust.select :close @16:01:03.85 SSV.dial CBV @16:01:03.88 CBV.dial SSV @16:01:04.68 Allowing 130s for 25.0ml of remaining 250.0ml @16:01:04.69 Intake.select :open @16:01:06.88 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:01:06.90 SS.reconfigure SSinhale.with maxSpeed: 19 @16:01:06.95 SS.seek 25ml @16:01:55.75 Vacuum too high -- waiting up to 40s @16:01:57.42 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:01:57.44 SS.reconfigure SSinhale.with maxSpeed: 17 @16:01:57.49 SS.seek 25ml @16:02:46.03 Stroke completed -- waiting for pressure to equalize ... @16:02:55.61 Intake.select :close @16:02:55.78 SSV.dial :exhaust @16:02:56.55 Exhaust.select :open @16:02:56.67 SS.reconfigure SSexhale @16:02:56.72 SS.seek 0ml @16:03:05.54 Exhaust.select :close @16:03:05.66 SSV.dial CBV @16:03:05.69 CBV.dial SSV @16:03:06.49 Allowing 130s for 25.0ml of remaining 225.0ml @16:03:06.50 Intake.select :open @16:03:08.69 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:03:08.71 SS.reconfigure SSinhale.with maxSpeed: 17 @16:03:08.76 SS.seek 25ml @16:04:52.01 Stroke completed -- waiting for pressure to equalize ... @16:05:02.87 Intake.select :close @16:05:03.03 SSV.dial :exhaust @16:05:03.80 Exhaust.select :open @16:05:03.91 SS.reconfigure SSexhale @16:05:03.97 SS.seek 0ml @16:05:12.98 Exhaust.select :close @16:05:13.10 SSV.dial CBV @16:05:13.13 CBV.dial SSV @16:05:13.93 Allowing 130s for 25.0ml of remaining 200.0ml @16:05:13.94 Intake.select :open @16:05:16.12 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:05:16.14 SS.reconfigure SSinhale.with maxSpeed: 17 @16:05:16.19 SS.seek 25ml @16:06:59.43 Stroke completed -- waiting for pressure to equalize ... @16:07:10.32 Intake.select :close @16:07:10.49 SSV.dial :exhaust @16:07:11.27 Exhaust.select :open @16:07:11.38 SS.reconfigure SSexhale @16:07:11.43 SS.seek 0ml @16:07:20.85 Exhaust.select :close @16:07:20.97 SSV.dial CBV @16:07:21.00 CBV.dial SSV @16:07:21.77 Allowing 130s for 25.0ml of remaining 175.0ml @16:07:21.78 Intake.select :open @16:07:23.96 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:07:23.98 SS.reconfigure SSinhale.with maxSpeed: 17 @16:07:24.03 SS.seek 25ml @16:08:41.06 Vacuum too high -- waiting up to 40s @16:08:43.57 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:08:43.59 SS.reconfigure SSinhale.with maxSpeed: 15 @16:08:43.64 SS.seek 25ml @16:09:13.17 Stroke completed -- waiting for pressure to equalize ... @16:09:24.09 Intake.select :close @16:09:24.30 SSV.dial :exhaust @16:09:25.07 Exhaust.select :open @16:09:25.18 SS.reconfigure SSexhale @16:09:25.24 SS.seek 0ml @16:09:34.35 Exhaust.select :close @16:09:34.47 SSV.dial CBV @16:09:34.50 CBV.dial SSV @16:09:35.28 Allowing 130s for 25.0ml of remaining 150.0ml @16:09:35.29 Intake.select :open @16:09:37.47 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:09:37.49 SS.reconfigure SSinhale.with maxSpeed: 15 @16:09:37.54 SS.seek 25ml @16:11:34.24 Stroke completed -- waiting for pressure to equalize ... @16:11:45.84 SS.reconfigure SSconfig @16:11:45.98 SS.hold @16:11:46.11 Intake.select :close @16:11:46.27 ShallowSampler::Clogged in WCR -- progress too slow after 1375.0ml @16:11:46.29 SSV.dial :exhaust @16:11:47.05 Exhaust.select :open @16:11:47.16 SS.reconfigure SSexhale @16:11:47.21 SS.seek 0ml @16:11:56.40 Exhaust.select :close @16:11:56.52 Sampled 1375.0ml @16:11:56.54 Drying puck before fixing for 4:00 @16:11:56.55 CBV.dial :waste2b @16:11:57.29 delay 1:00 @16:12:57.32 CSV.dial :air @16:12:57.87 CS.reconfigure CSfast @16:12:57.93 CS.seek 10ml @16:13:20.51 Pumping wcr puck from tube 2 up to 55psi... @16:13:20.52 CBV.dial SSV @16:13:21.84 CBV.dial between CSV and waste2b @16:13:22.80 CSV.dial between air and CTV @16:13:23.13 CS.seek 6.859ml @16:13:31.04 CTV.dial CSV,avoiding: :air @16:13:31.07 CSV.dial CTV @16:13:31.87 CS.reconfigure CSpressurize @16:13:31.93 CS.seek 0ml @16:13:50.06 CSV.dial :air @16:13:50.53 CS.reconfigure CSfast @16:13:50.59 CS.seek 10ml @16:14:12.98 CSV.dial between air and CTV @16:14:13.32 CS.seek 2.251ml @16:14:31.01 CTV.dial CSV,avoiding: :air @16:14:31.04 CSV.dial CTV @16:14:31.37 CS.reconfigure CSpressurize @16:14:31.42 CS.seek 0ml @16:14:34.13 CBV.dial :waste2b @16:14:34.47 CS.reconfigure CSconfig @16:14:34.52 CS.seek :empty @16:14:39.08 delay 1:00 @16:15:39.11 CTV.dial :air,avoiding: :intake @16:15:40.39 Fix #1 of 2 (with rnal) for 2:00 @16:15:40.41 CSV.dial CSR @16:15:40.90 Pulling 2ml of CSR.rnal @16:15:40.91 CSR.select :rnal @16:15:41.04 CS.seek 2ml @16:15:47.20 delay 5 seconds @16:15:52.22 CSR.select :air @16:15:52.35 CS.reconfigure CSfast @16:15:52.41 CS.seek 10ml @16:16:10.42 Pumping wcr puck from tube 2 up to 55psi... @16:16:11.01 CBV.dial SSV @16:16:12.33 CBV.dial between CSV and waste2b @16:16:13.29 CSV.dial between air and CTV @16:16:13.90 CS.seek 6.789ml @16:16:21.83 CTV.dial CSV,avoiding: :air @16:16:21.86 CSV.dial CTV @16:16:23.23 CS.reconfigure CSpressurize @16:16:23.29 CS.seek 0ml @16:16:41.13 delay 2:00 @16:18:41.16 CBV.dial :waste2b @16:18:41.50 CS.reconfigure CSconfig @16:18:41.56 CS.seek :empty @16:18:42.05 delay 1:00 @16:19:42.08 CTV.dial :air,avoiding: :intake @16:19:43.34 Fix #2 of 2 (with rnal) for 2:00 @16:19:43.36 CSV.dial CSR @16:19:43.86 Pulling 2ml of CSR.rnal @16:19:43.88 CSR.select :rnal @16:19:44.00 CS.seek 2ml @16:19:50.11 delay 5 seconds @16:19:55.13 CSR.select :air @16:19:55.26 CS.reconfigure CSfast @16:19:55.32 CS.seek 10ml @16:20:13.30 Pumping wcr puck from tube 2 up to 55psi... @16:20:13.31 CBV.dial SSV @16:20:14.62 CBV.dial between CSV and waste2b @16:20:15.59 CSV.dial between air and CTV @16:20:16.20 CS.seek 6.793ml @16:20:24.30 CTV.dial CSV,avoiding: :air @16:20:24.33 CSV.dial CTV @16:20:25.66 CS.reconfigure CSpressurize @16:20:25.72 CS.seek 0ml @16:20:42.20 delay 2:00 @16:22:42.22 CBV.dial :waste2b @16:22:42.57 CS.reconfigure CSconfig @16:22:42.62 CS.seek :empty @16:22:44.89 delay 1:00 @16:23:44.91 CTV.dial :air,avoiding: :intake @16:23:46.26 Storing wcr puck in Collection Clamp to tube 1 @16:23:46.45 SC.reconfigure SCtight @16:23:46.51 SC.seek 1 @16:23:48.61 SC.reconfigure SCloose @16:23:48.85 Elbow.reconfigure TightElbow @16:23:48.90 Elbow.seek 1 @16:23:50.83 Forearm.reconfigure TightForearm @16:23:50.89 Forearm.seek 1 + 700 counts @16:23:51.93 SE.reconfigure SEpick @16:23:52.00 SE.seek beamBreak @16:23:54.39 SE.configure SEconfig @16:23:54.56 Elbow.reconfigure LimpElbow @16:23:54.62 Forearm.reconfigure LooseForearm @16:23:54.67 Forearm.seek 1 @16:23:54.99 Elbow.hold @16:23:55.06 Elbow.reconfigure TightElbow @16:23:55.17 SE.seek 7.09 Pucks below Top Plate @16:23:55.64 Forearm.force -20 @16:23:55.69 Forearm.seek 1 + 334 counts @16:23:56.04 Forearm.reconfigure TightForearm @16:23:56.10 Forearm.seek 1 + 700 counts @16:23:56.36 SE.seek 8.09 Pucks below Top Plate @16:23:57.04 Forearm.reconfigure LooseForearm @16:23:57.09 Forearm.seek :retract @16:23:58.01 Elbow.seek CC @16:23:59.72 Elbow.reconfigure LooseElbow @16:23:59.83 Forearm.seek CC @16:24:00.80 Hand.close @16:24:01.40 CC.seek :open @16:24:05.88 Forearm.seek :retract @16:24:06.85 Elbow.reconfigure TightElbow @16:24:06.90 Elbow.seek 1 @16:24:08.82 Elbow.reconfigure LimpElbow @16:24:08.88 Forearm.seek 1 @16:24:09.90 Elbow.hold @16:24:09.98 Elbow.reconfigure TightElbow @16:24:10.03 Hand.open @16:24:10.78 Forearm.force -20 @16:24:11.42 Forearm.seek 1 + 346 counts @16:24:11.77 Forearm.reconfigure TightForearm @16:24:11.83 Forearm.seek 1 + 700 counts @16:24:12.16 SE.seek :down @16:24:13.34 Forearm.reconfigure LooseForearm @16:24:13.40 Forearm.seek :retract @16:24:14.12 SE.coast @16:24:14.35 SC.coast @16:24:14.39 Starting finalPurge @16:24:14.41 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:24:14.49 Hand.open @16:24:14.92 Elbow.seek FlushPuck::Garage @16:24:16.87 Elbow.reconfigure LooseElbow @16:24:16.98 Forearm.seek :garage @16:24:17.99 Hand.close @16:24:18.79 Forearm.seek :retract @16:24:19.79 Elbow.reconfigure TightElbow @16:24:19.84 Elbow.seek CC @16:24:22.76 Elbow.reconfigure LooseElbow @16:24:22.86 Forearm.reconfigure TightForearm @16:24:22.91 Forearm.seek CC @16:24:24.18 CC.seek :closed @16:24:28.46 Collection Clamp onSpring/offSpring for 6/32 samples, on/off torque 1.78 @16:24:28.53 Hand.open @16:24:29.22 Forearm.reconfigure LooseForearm @16:24:29.27 Forearm.seek :retract @16:24:30.24 Flushing the Collection Valve::Manifold @16:24:30.38 CSV.dial CSR @16:24:30.88 Pulling 5ml of CSR.flush @16:24:30.89 CSR.select :flush @16:24:31.02 CS.seek 5ml @16:24:45.02 delay 5 seconds @16:24:50.04 CSR.select :air @16:24:50.22 CS.reconfigure CSfast @16:24:50.26 CS.seek 7.5ml @16:24:56.60 CSV.dial :waste @16:24:57.85 CS.reconfigure CSconfig @16:24:57.90 CS.seek 0ml @16:25:18.08 Flushing lower manifold's waste1 port @16:25:18.22 CSV.dial :flush @16:25:18.66 CS.reconfigure CSfast @16:25:18.71 CS.seek 5ml @16:25:30.40 delay 5 seconds @16:25:35.41 CSV.dial :air @16:25:36.56 CS.seek 7ml @16:25:41.96 CSV.dial CTV @16:25:41.99
CTV.dial CSV @16:25:43.35 CBV.dial :waste1 @16:25:44.13 CS.seek 0ml @16:25:59.96 Flushing lower manifold's waste2b port @16:26:00.10 CSV.dial :flush @16:26:00.84 CS.seek 5ml @16:26:12.43 delay 5 seconds @16:26:17.44 CSV.dial :air @16:26:17.99 CS.seek 7ml @16:26:23.27 CSV.dial CTV @16:26:23.30
CTV.dial CSV @16:26:23.76 CBV.dial :waste2b @16:26:24.52 CS.seek 0ml @16:26:40.34 Flushing upper manifold @16:26:40.48 CSV.dial :flush @16:26:41.22 CS.seek 5ml @16:26:52.90 delay 5 seconds @16:26:57.92 CSV.dial :air @16:26:58.58 CS.seek 7ml @16:27:03.90 CSV.dial CBV @16:27:03.93
CBV.dial CSV @16:27:04.98 CTV.dial :air @16:27:06.27 CS.seek 0ml @16:27:22.40 delay 7 seconds @16:27:29.43 CS.seek 3ml @16:27:36.83 CSV.dial :waste @16:27:37.30 CS.seek 0ml @16:27:44.77 CSV.dial :flush @16:27:45.23 CS.seek 3ml @16:27:52.74 delay 5 seconds @16:27:57.75 CSV.dial :air @16:27:58.28 CS.seek 5.001ml @16:28:03.70 CSV.dial :air @16:28:03.73 CS.seek 0ml @16:28:15.43 delay 7 seconds @16:28:22.46 CS.seek 3ml @16:28:29.92 CSV.dial :waste @16:28:30.66 CS.seek 0ml @16:28:38.06 Disinfecting external Intake with mfbkill @16:28:38.19 CSV.dial CSR @16:28:39.53 Pulling 8ml of CSR.mfbkill @16:28:39.54 CSR.select :mfbkill @16:28:39.67 CS.seek 8ml @16:28:57.46 delay 5 seconds @16:29:02.48 CSR.select :air @16:29:02.62 CS.seek 10ml @16:29:07.97 CSV.dial CBV @16:29:07.99
CBV.dial CSV @16:29:08.98 CTV.dial :intake @16:29:09.70 Intake.select :open @16:29:09.83 CS.seek 2ml @16:29:28.58 Intake.select :close @16:29:28.70 CTV.dial :air @16:29:29.45 CS.reconfigure CSconfig @16:29:29.50 CS.seek 0ml @16:29:36.27 Flushing lower manifold's waste1 port @16:29:36.28 CSV.dial CSR @16:29:37.35 Pulling 4ml of CSR.mfbkill @16:29:37.37 CSR.select :mfbkill @16:29:37.51 CS.seek 4ml @16:29:48.74 delay 5 seconds @16:29:53.76 CSR.select :air @16:29:53.89 CS.reconfigure CSfast @16:29:53.94 CS.seek 10ml @16:30:07.65 CSV.dial CTV @16:30:07.68
CTV.dial CSV @16:30:09.06 CBV.dial :waste1 @16:30:09.54 CS.seek 0ml @16:30:31.65 Flushing lower manifold's waste2b port @16:30:31.66 CSV.dial CSR @16:30:32.19 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @16:30:32.32 CS.reconfigure CSconfig @16:30:32.37 CS.seek 4ml @16:30:43.68 delay 5 seconds @16:30:48.70 CSR.select :air @16:30:48.83 CS.reconfigure CSfast @16:30:48.88 CS.seek 10ml @16:31:02.62 CSV.dial CTV @16:31:02.65
CTV.dial CSV @16:31:03.14 CBV.dial :waste2b @16:31:03.90 CS.seek 0ml @16:31:25.93 Killing upper manifold @16:31:25.94 CSV.dial CSR @16:31:26.45 Pulling 4ml of CSR.mfbkill @16:31:26.46 CSR.select :mfbkill @16:31:26.59 CS.reconfigure CSconfig @16:31:26.63 CS.seek 4ml @16:31:37.87 delay 5 seconds @16:31:42.88 CSR.select :air @16:31:43.01 CS.reconfigure CSfast @16:31:43.06 CS.seek 10ml @16:31:56.84 CSV.dial CBV @16:31:56.87
CBV.dial CSV @16:31:57.92 CTV.dial :air @16:31:59.21 CS.seek 0ml @16:32:21.31 delay 7 seconds @16:32:28.33 CS.seek 3ml @16:32:35.70 CSV.dial :waste @16:32:36.27 CS.seek 0ml @16:32:43.72 Flushing upper manifold @16:32:43.86 CSV.dial :flush @16:32:44.33 CS.seek 5ml @16:32:56.03 delay 5 seconds @16:33:01.04 CSV.dial :air @16:33:01.60 CS.seek 7ml @16:33:07.53 CSV.dial CBV @16:33:07.57
CBV.dial CSV @16:33:08.56 CTV.dial :air @16:33:08.58 CS.seek 0ml @16:33:24.40 delay 7 seconds @16:33:31.42 CS.seek 3ml @16:33:38.86 CSV.dial :waste @16:33:39.32 CS.seek 0ml @16:33:46.84 CSV.dial :flush @16:33:47.91 CS.seek 3ml @16:33:55.40 delay 5 seconds @16:34:00.41 CSV.dial :air @16:34:00.94 CS.seek 5ml @16:34:06.34 CSV.dial :air @16:34:06.36 CS.seek 0ml @16:34:17.97 delay 7 seconds @16:34:24.99 CS.seek 3ml @16:34:32.39 CSV.dial :waste @16:34:33.12 CS.seek 0ml @16:34:40.56 Flushing lower manifold's waste1 port @16:34:40.70 CSV.dial :flush @16:34:41.16 CS.seek 5ml @16:34:52.72 delay 5 seconds @16:34:57.74 CSV.dial :air @16:34:58.27 CS.seek 7ml @16:35:03.59 CSV.dial CTV @16:35:03.62
CTV.dial CSV @16:35:05.00 CBV.dial :waste1 @16:35:05.48 CS.seek 0ml @16:35:21.28 Flushing lower manifold's waste2b port @16:35:21.42 CSV.dial :flush @16:35:22.16 CS.seek 5ml @16:35:33.84 delay 5 seconds @16:35:38.85 CSV.dial :air @16:35:39.38 CS.seek 7ml @16:35:44.72 CSV.dial CTV @16:35:44.75
CTV.dial CSV @16:35:45.33 CBV.dial :waste2b @16:35:46.09 CS.seek 0ml @16:36:01.78 Disinfecting external Intake with kill @16:36:01.92 CSV.dial CSR @16:36:02.45 Pulling 8ml of CSR.kill @16:36:02.46 CSR.select :kill @16:36:02.59 CS.seek 8ml @16:36:20.53 delay 5 seconds @16:36:25.55 CSR.select :air @16:36:25.69 CS.seek 10ml @16:36:31.09 CSV.dial CBV @16:36:31.12
CBV.dial CSV @16:36:32.17 CTV.dial :intake @16:36:32.89 Intake.select :open @16:36:33.01 CS.seek 2ml @16:36:51.58 Intake.select :close @16:36:51.70 CTV.dial :air @16:36:52.42 CS.reconfigure CSconfig @16:36:52.47 CS.seek 0ml @16:36:58.66 CSV.dial CSR @16:36:59.72 Pulling 5ml of CSR.flush @16:36:59.73 CSR.select :flush @16:36:59.86 CS.seek 5ml @16:37:13.59 delay 5 seconds @16:37:18.61 CSR.select :air @16:37:18.75 CS.seek 10ml @16:37:32.46 CSV.dial CBV @16:37:33.45 CS.seek 0ml @16:37:59.91 Garaging FlushPuck in Collection Clamp @16:38:00.60 Elbow.reconfigure TightElbow @16:38:00.65 Elbow.seek CC @16:38:00.91 Elbow.reconfigure LooseElbow @16:38:01.01 Forearm.seek CC @16:38:02.01 Hand.close @16:38:02.67 CC.seek :open @16:38:07.04 Forearm.seek :retract @16:38:08.06 Elbow.reconfigure TightElbow @16:38:08.11 Elbow.seek :garage @16:38:11.23 Elbow.reconfigure LooseElbow @16:38:11.34 Forearm.reconfigure TightForearm @16:38:11.39 Forearm.seek :garage @16:38:12.55 Hand.open @16:38:13.24 Forearm.reconfigure LooseForearm @16:38:13.29 Forearm.seek :retract @16:38:14.35 Starting initialPurge @16:38:14.36 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:38:14.44 Hand.open @16:38:14.74 Elbow.reconfigure TightElbow @16:38:14.78 Elbow.seek FlushPuck::Garage @16:38:15.04 Elbow.reconfigure LooseElbow @16:38:15.15 Forearm.seek :garage @16:38:16.17 Hand.close @16:38:16.93 Forearm.seek :retract @16:38:17.97 Elbow.reconfigure TightElbow @16:38:18.02 Elbow.seek CC @16:38:21.01 Elbow.reconfigure LooseElbow @16:38:21.12 Forearm.reconfigure TightForearm @16:38:21.17 Forearm.seek CC @16:38:22.48 CC.seek :closed @16:38:26.74 Collection Clamp onSpring/offSpring for 6/32 samples, on/off torque 1.77 @16:38:26.82 Hand.open @16:38:27.49 Forearm.reconfigure LooseForearm @16:38:27.53 Forearm.seek :retract @16:38:28.50 Flushing the Collection Valve::Manifold @16:38:28.63 CSV.dial CSR @16:38:29.79 Pulling 5ml of CSR.flush @16:38:29.80 CSR.select :flush @16:38:29.93 CS.seek 5ml @16:38:43.72 delay 5 seconds @16:38:48.74 CSR.select :air @16:38:48.92 CS.reconfigure CSfast @16:38:48.96 CS.seek 7.501ml @16:38:55.24 CSV.dial :waste @16:38:56.49 CS.reconfigure CSconfig @16:38:56.55 CS.seek 0ml @16:39:16.69 Purging external Intake @16:39:16.82 CSV.dial :flush @16:39:17.27 CS.reconfigure CSfast @16:39:17.32 CS.seek 10ml @16:39:39.90 delay 5 seconds @16:39:44.97 CSV.dial CBV @16:39:45.00
CBV.dial CSV @16:39:45.74 CTV.dial :intake @16:39:46.49 Intake.select :open @16:39:46.61 CS.seek 0ml @16:40:09.30 Intake.select :close @16:40:09.42 CTV.dial :air @16:40:10.14 Flushing upper manifold @16:40:10.28 CSV.dial :flush @16:40:11.01 CS.seek 5ml @16:40:22.63 delay 5 seconds @16:40:27.64 CSV.dial :air @16:40:28.18 CS.seek 7ml @16:40:33.57 CSV.dial CBV @16:40:33.60
CBV.dial CSV @16:40:34.60 CTV.dial :air @16:40:34.62 CS.seek 0ml @16:40:50.41 delay 7 seconds @16:40:57.43 CS.seek 3ml @16:41:04.81 CSV.dial :waste @16:41:05.27 CS.seek 0ml @16:41:13.15 CSV.dial :flush @16:41:13.61 CS.seek 3ml @16:41:21.07 delay 5 seconds @16:41:26.08 CSV.dial :air @16:41:26.59 CS.seek 5ml @16:41:31.86 CSV.dial :air @16:41:31.88 CS.seek 0ml @16:41:43.38 delay 7 seconds @16:41:50.40 CS.seek 3ml @16:41:57.84 CSV.dial :waste @16:41:58.58 CS.seek 0ml @16:42:06.04 Garaging FlushPuck in Collection Clamp @16:42:06.12 Elbow.reconfigure TightElbow @16:42:06.17 Elbow.seek CC @16:42:06.43 Elbow.reconfigure LooseElbow @16:42:06.54 Forearm.seek CC @16:42:07.54 Hand.close @16:42:08.17 CC.seek :open @16:42:12.51 Forearm.seek :retract @16:42:13.51 Elbow.reconfigure TightElbow @16:42:13.56 Elbow.seek :garage @16:42:16.65 Elbow.reconfigure LooseElbow @16:42:16.76 Forearm.reconfigure TightForearm @16:42:16.81 Forearm.seek :garage @16:42:17.97 Hand.open @16:42:18.62 Forearm.reconfigure LooseForearm @16:42:18.67 Forearm.seek :retract @16:42:19.66 Completed [coreNEG] begun 14:00:23 @16:42:19.68 Preparing to sleep until 14PM @16:42:19.72 deferred until MFBPCR finish @16:42:19.75
Waiting for MFBPCR til 14PM @16:42:19.78 Continuing at 14:00CEST03-Oct-18 @16:42:19.83 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/18tundeployv2.rb:115\n{1 => 9, 2 => 13}\nCan@16:42:19, 15.6C, 67% humidity, 14.4psia, 14.488V, 0.689A, 0.682A avg, 9.98W",Subject: "Waiting until 14:00CEST03-Oct-18" @17:20:44.00 PCR.off @17:20:46.16 PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0215h.pcr @17:20:46.23 Cleaning PCR @17:20:46.24 AFV.select :carrierSolution @17:20:46.36 delay 2 seconds @17:20:48.39 AS.reconfigure ASconfig @17:20:48.44 AS.seek 0.5ml @17:20:58.73 delay 1 second @17:20:59.74 AFV.select :holdingCoil @17:20:59.85 ASV.dial :waste1 @17:21:00.21 ATV.dial :air3 @17:21:01.39 AS.reconfigure ASslow1 @17:21:01.44 AS.seek 0.51ml @17:21:03.71 delay 2 seconds @17:21:05.73 ATV.dial :bleach @17:21:06.33 AS.seek 0.81ml @17:22:06.92 delay 3 seconds @17:22:09.94 ATV.dial :air3 @17:22:10.46 delay 3 seconds @17:22:13.55 AS.seek 0.835ml @17:22:18.83 delay 3 seconds @17:22:21.85 ATV.dial :bleach @17:22:22.46 AS.seek 0.82ml @17:22:25.76 delay 3 seconds @17:22:28.78 ARV.dial :waste @17:22:29.37 delay 3 seconds @17:22:32.39 ATV.dial :ARV @17:22:33.32 AS.reconfigure ASconfig @17:22:33.37 AS.seek 0.47ml @17:22:53.75 AFV.select :carrierSolution @17:22:53.87 delay 2 seconds @17:22:55.90 AS.seek 1ml @17:23:26.64 delay 1 second @17:23:27.65 AFV.select :holdingCoil @17:23:27.76 delay 1 second @17:23:28.79 AS.reconfigure ASfast @17:23:28.84 AS.seek 0ml @17:23:56.17 AFV.select :carrierSolution @17:23:56.29 delay 2 seconds @17:23:58.32 AS.reconfigure ASconfig @17:23:58.37 AS.seek 1ml @17:24:56.12 delay 1 second @17:24:57.13 AFV.select :holdingCoil @17:24:57.24 delay 1 second @17:24:58.27 AS.reconfigure ASfast @17:24:58.32 AS.seek 0ml @17:25:25.66 AFV.select :carrierSolution @17:25:25.78 delay 2 seconds @17:25:27.81 AS.reconfigure ASconfig @17:25:27.86 AS.seek 1ml @17:26:25.64 delay 1 second @17:26:26.65 AFV.select :holdingCoil @17:26:26.76 delay 1 second @17:26:27.79 AS.reconfigure ASfast @17:26:27.84 AS.seek 0ml @17:26:55.16 ARV.dial between carrier and air1 @17:26:55.65 ATV.dial :air3 @17:26:56.85 AS.reconfigure ASslow1 @17:26:56.90 AS.seek 0.01ml @17:26:59.25 delay 2 seconds @17:27:01.27 ATV.dial :bleach @17:27:01.86 AS.seek 0.81ml @17:29:43.00 delay 3 seconds @17:29:46.02 ATV.dial :air3 @17:29:46.54 delay 3 seconds @17:29:49.63 AS.seek 0.835ml @17:29:54.93 delay 3 seconds @17:29:57.95 ATV.dial :bleach @17:29:58.55 AS.seek 0.82ml @17:30:01.89 delay 3 seconds @17:30:04.91 ATV.dial PCR @17:30:05.29 AS.reconfigure ASconfig @17:30:05.34 AS.seek :empty @17:30:52.80 PCR.program :bleachProfile @17:30:52.87 PCR program should complete in < 2:45 @17:30:52.88 PCR.run 10:00 @17:34:00.76 PCR.off @17:34:00.78 AFV.select :carrierSolution @17:34:00.90 delay 2 seconds @17:34:02.93 AS.seek 1ml @17:35:00.76 delay 1 second @17:35:01.77 AFV.select :holdingCoil @17:35:01.88 delay 1 second @17:35:02.91 AS.reconfigure ASfast @17:35:02.96 AS.seek 0ml @17:35:30.91 delay 3 seconds @17:35:33.93 AFV.select :carrierSolution @17:35:34.04 delay 2 seconds @17:35:36.07 AS.reconfigure ASconfig @17:35:36.12 AS.seek 1ml @17:36:33.91 delay 1 second @17:36:34.92 AFV.select :holdingCoil @17:36:35.03 delay 1 second @17:36:36.06 AS.reconfigure ASfast @17:36:36.12 AS.seek 0ml @17:37:03.47 delay 3 seconds @17:37:06.49 AFV.select :carrierSolution @17:37:06.60 delay 2 seconds @17:37:08.63 AS.reconfigure ASconfig @17:37:08.69 AS.seek 1ml @17:38:06.45 delay 1 second @17:38:07.46 AFV.select :holdingCoil @17:38:07.57 delay 1 second @17:38:08.60 AS.reconfigure ASfast @17:38:08.65 AS.seek 0ml @17:38:36.00 delay 3 seconds @17:38:39.02 AFV.select :carrierSolution @17:38:39.13 delay 2 seconds @17:38:41.16 AS.reconfigure ASconfig @17:38:41.21 AS.seek 1ml @17:39:39.05 delay 1 second @17:39:40.06 AFV.select :holdingCoil @17:39:40.17 delay 1 second @17:39:41.20 AS.reconfigure ASfast @17:39:41.25 AS.seek 0ml @17:40:08.61 delay 3 seconds @17:40:11.63 AFV.select :carrierSolution @17:40:11.74 delay 2 seconds @17:40:13.78 AS.reconfigure ASconfig @17:40:13.84 AS.seek 1ml @17:41:11.64 delay 1 second @17:41:12.65 AFV.select :holdingCoil @17:41:12.76 delay 1 second @17:41:13.79 AS.reconfigure ASnorm @17:41:13.84 AS.seek 0ml @17:42:04.57 Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0217h.pcr @17:42:04.59 Building PCR Slug from ASV with MM2 mix, PP3 primer @17:42:04.60 Priming with carrier @17:42:04.61 ATV.dial ARV @17:42:05.28 ARV.dial :carrier @17:42:05.63 AS.seek 0.5ml @17:42:31.18 AFV.select :carrierSolution @17:42:31.30 delay 2 seconds @17:42:33.33 AS.reconfigure ASconfig @17:42:33.38 AS.seek 1ml @17:43:02.39 delay 1 second @17:43:03.40 AFV.select :holdingCoil @17:43:03.51 delay 1 second @17:43:04.53 ATV.dial :waste @17:43:06.05 AS.reconfigure ASnorm @17:43:06.10 AS.seek :empty @17:43:56.82 AFV.select :carrierSolution @17:43:56.94 delay 2 seconds @17:43:58.97 AS.reconfigure ASconfig @17:43:59.02 AS.seek 0.5ml @17:44:28.13 delay 1 second @17:44:29.14 AFV.select :holdingCoil @17:44:29.25 Building Slug @17:44:29.26 ATV.dial :ARV @17:44:30.75 delay 2 seconds @17:44:32.77 ARV.dial :air1 @17:44:33.26 delay 2 seconds @17:44:35.35 AS.reconfigure ASslow2 @17:44:35.40 AS.seek 0.506ml @17:44:41.67 delay 2 seconds @17:44:43.68 ARV.dial :MM2 @17:44:45.08 delay 2 seconds @17:44:47.11 Adding MM2 @17:44:47.18 AS.seek 0.524ml @17:45:05.42 delay 25 seconds @17:45:30.44 ARV.dial :PP3 @17:45:30.83 Adding PP3 @17:45:30.85 delay 2 seconds @17:45:32.93 AS.seek 0.53ml @17:45:39.40 delay 10 seconds @17:45:49.43 ARV.dial :air2 @17:45:49.81 adding ARV air @17:45:49.83 delay 2 seconds @17:45:51.91 AS.seek 0.536ml @17:45:58.22 delay 5 seconds @17:46:03.24 ARV.dial :carrier @17:46:04.44 delay 7 seconds @17:46:11.53 AS.seek 0.594ml @17:47:10.19 delay 5 seconds @17:47:15.21 ATV.dial :ASV @17:47:15.60 delay 2 seconds @17:47:17.69 AS.seek 0.6ml @17:47:24.00 delay 4 seconds @17:47:28.02 ATV.dial ARV @17:47:28.53 delay 2 seconds @17:47:30.55 ARV.dial :carrier @17:47:30.57 delay 2 seconds @17:47:32.66 AS.seek 0.65ml @17:48:23.30 delay 4 seconds @17:48:27.32 ATV.dial PCR @17:48:27.88 ASV.dial :waste1 @17:48:27.90 ARV.dial :air1 @17:48:28.28 Positioning Slug by 0.324ml @17:48:28.38 AS.seek 0.326ml @17:53:54.35 delay 5 seconds @17:53:59.37 ATV.dial ARV @17:54:00.10 ASV.dial :PCRlock @17:54:00.47 delay 5 seconds @17:54:05.50 Running Rp Profile60C300 thermocycling @17:54:05.51 PCR.program Profile60C300 @17:54:05.65 Profile60C300 should complete in < 1:45:21 @17:54:05.66 PCR.run 2:16:41.25 @19:16:04.90 PCR.off @19:16:07.08 PCR for Rp completed -- closed /var/log/esp/Rp18oct0217h.pcr @19:16:07.15 Cleaning PCR @19:16:07.16 AFV.select :carrierSolution @19:16:07.27 delay 2 seconds @19:16:09.30 AS.reconfigure ASconfig @19:16:09.35 AS.seek 0.5ml @19:16:19.65 delay 1 second @19:16:20.66 AFV.select :holdingCoil @19:16:20.77 ASV.dial :waste1 @19:16:21.13 ATV.dial :air3 @19:16:22.33 AS.reconfigure ASslow1 @19:16:22.38 AS.seek 0.51ml @19:16:24.62 delay 2 seconds @19:16:26.63 ATV.dial :bleach @19:16:27.24 AS.seek 0.81ml @19:17:27.84 delay 3 seconds @19:17:30.86 ATV.dial :air3 @19:17:31.39 delay 3 seconds @19:17:34.48 AS.seek 0.835ml @19:17:39.80 delay 3 seconds @19:17:42.82 ATV.dial :bleach @19:17:43.42 AS.seek 0.82ml @19:17:46.79 delay 3 seconds @19:17:49.81 ARV.dial :waste @19:17:50.40 delay 3 seconds @19:17:53.42 ATV.dial :ARV @19:17:54.35 AS.reconfigure ASconfig @19:17:54.41 AS.seek 0.47ml @19:18:14.80 AFV.select :carrierSolution @19:18:14.92 delay 2 seconds @19:18:16.95 AS.seek 1ml @19:18:47.70 delay 1 second @19:18:48.71 AFV.select :holdingCoil @19:18:48.82 delay 1 second @19:18:49.85 AS.reconfigure ASfast @19:18:49.90 AS.seek 0ml @19:19:17.25 AFV.select :carrierSolution @19:19:17.37 delay 2 seconds @19:19:19.40 AS.reconfigure ASconfig @19:19:19.45 AS.seek 1ml @19:20:17.20 delay 1 second @19:20:18.21 AFV.select :holdingCoil @19:20:18.32 delay 1 second @19:20:19.35 AS.reconfigure ASfast @19:20:19.40 AS.seek 0ml @19:20:46.74 AFV.select :carrierSolution @19:20:46.86 delay 2 seconds @19:20:48.89 AS.reconfigure ASconfig @19:20:48.94 AS.seek 1ml @19:21:46.68 delay 1 second @19:21:47.69 AFV.select :holdingCoil @19:21:47.80 delay 1 second @19:21:48.83 AS.reconfigure ASfast @19:21:48.88 AS.seek 0ml @19:22:16.20 ARV.dial between carrier and air1 @19:22:16.68 ATV.dial :air3 @19:22:17.88 AS.reconfigure ASslow1 @19:22:17.93 AS.seek 0.01ml @19:22:20.28 delay 2 seconds @19:22:22.29 ATV.dial :bleach @19:22:22.89 AS.seek 0.81ml @19:25:04.04 delay 3 seconds @19:25:07.06 ATV.dial :air3 @19:25:07.59 delay 3 seconds @19:25:10.68 AS.seek 0.835ml @19:25:15.97 delay 3 seconds @19:25:19.00 ATV.dial :bleach @19:25:19.60 AS.seek 0.82ml @19:25:22.90 delay 3 seconds @19:25:25.92 ATV.dial PCR @19:25:26.31 AS.reconfigure ASconfig @19:25:26.36 AS.seek :empty @19:26:13.82 PCR.program :bleachProfile @19:26:13.88 PCR program should complete in < 2:45 @19:26:13.89 PCR.run 10:00 @19:29:21.77 PCR.off @19:29:21.80 AFV.select :carrierSolution @19:29:21.91 delay 2 seconds @19:29:23.94 AS.seek 1ml @19:30:21.76 delay 1 second @19:30:22.77 AFV.select :holdingCoil @19:30:22.88 delay 1 second @19:30:23.91 AS.reconfigure ASfast @19:30:23.96 AS.seek 0ml @19:30:51.97 delay 3 seconds @19:30:54.99 AFV.select :carrierSolution @19:30:55.10 delay 2 seconds @19:30:57.13 AS.reconfigure ASconfig @19:30:57.19 AS.seek 1ml @19:31:54.98 delay 1 second @19:31:55.99 AFV.select :holdingCoil @19:31:56.10 delay 1 second @19:31:57.13 AS.reconfigure ASfast @19:31:57.18 AS.seek 0ml @19:32:24.51 delay 3 seconds @19:32:27.53 AFV.select :carrierSolution @19:32:27.64 delay 2 seconds @19:32:29.67 AS.reconfigure ASconfig @19:32:29.72 AS.seek 1ml @19:33:27.51 delay 1 second @19:33:28.52 AFV.select :holdingCoil @19:33:28.63 delay 1 second @19:33:29.66 AS.reconfigure ASfast @19:33:29.71 AS.seek 0ml @19:33:57.06 delay 3 seconds @19:34:00.08 AFV.select :carrierSolution @19:34:00.19 delay 2 seconds @19:34:02.22 AS.reconfigure ASconfig @19:34:02.30 AS.seek 1ml @19:35:00.09 delay 1 second @19:35:01.10 AFV.select :holdingCoil @19:35:01.21 delay 1 second @19:35:02.24 AS.reconfigure ASfast @19:35:02.29 AS.seek 0ml @19:35:29.62 delay 3 seconds @19:35:32.64 AFV.select :carrierSolution @19:35:32.75 delay 2 seconds @19:35:34.78 AS.reconfigure ASconfig @19:35:34.83 AS.seek 1ml @19:36:32.62 delay 1 second @19:36:33.63 AFV.select :holdingCoil @19:36:33.74 delay 1 second @19:36:34.77 AS.reconfigure ASnorm @19:36:34.82 AS.seek 0ml @19:37:25.53 Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0219h.pcr @19:37:25.55 Building PCR Slug from ASV with MM3 mix, PP5 primer @19:37:25.57 Priming with carrier @19:37:25.58 ATV.dial ARV @19:37:26.16 ARV.dial :carrier @19:37:26.51 AS.seek 0.5ml @19:37:52.08 AFV.select :carrierSolution @19:37:52.20 delay 2 seconds @19:37:54.23 AS.reconfigure ASconfig @19:37:54.28 AS.seek 1ml @19:38:23.28 delay 1 second @19:38:24.29 AFV.select :holdingCoil @19:38:24.40 delay 1 second @19:38:25.42 ATV.dial :waste @19:38:26.95 AS.reconfigure ASnorm @19:38:27.00 AS.seek :empty @19:39:17.72 AFV.select :carrierSolution @19:39:17.84 delay 2 seconds @19:39:19.87 AS.reconfigure ASconfig @19:39:19.92 AS.seek 0.5ml @19:39:49.03 delay 1 second @19:39:50.04 AFV.select :holdingCoil @19:39:50.15 Building Slug @19:39:50.16 ATV.dial :ARV @19:39:51.66 delay 2 seconds @19:39:53.68 ARV.dial :air1 @19:39:54.07 delay 2 seconds @19:39:56.16 AS.reconfigure ASslow2 @19:39:56.21 AS.seek 0.506ml @19:40:02.44 delay 2 seconds @19:40:04.46 ARV.dial :MM3 @19:40:05.45 delay 2 seconds @19:40:07.48 Adding MM3 @19:40:07.56 AS.seek 0.524ml @19:40:25.95 delay 25 seconds @19:40:50.97 ARV.dial :PP5 @19:40:51.36 Adding PP5 @19:40:51.37 delay 2 seconds @19:40:53.46 AS.seek 0.53ml @19:40:59.91 delay 10 seconds @19:41:09.93 ARV.dial :air3 @19:41:10.31 adding ARV air @19:41:10.32 delay 2 seconds @19:41:12.41 AS.seek 0.536ml @19:41:18.69 delay 5 seconds @19:41:23.71 ARV.dial :carrier @19:41:24.91 delay 7 seconds @19:41:32.00 AS.seek 0.594ml @19:42:30.77 delay 5 seconds @19:42:35.79 ATV.dial :ASV @19:42:36.28 delay 2 seconds @19:42:38.37 AS.seek 0.6ml @19:42:44.75 delay 4 seconds @19:42:48.77 ATV.dial ARV @19:42:49.28 delay 2 seconds @19:42:51.30 ARV.dial :carrier @19:42:51.32 delay 2 seconds @19:42:53.41 AS.seek 0.65ml @19:43:43.89 delay 4 seconds @19:43:47.91 ATV.dial PCR @19:43:48.49 ASV.dial :waste1 @19:43:48.50 ARV.dial :air1 @19:43:48.99 Positioning Slug by 0.324ml @19:43:49.08 AS.seek 0.326ml @19:49:15.94 delay 5 seconds @19:49:20.96 ATV.dial ARV @19:49:21.67 ASV.dial :PCRlock @19:49:22.03 delay 5 seconds @19:49:27.06 Running Bb Profile60C300 thermocycling @19:49:27.07 PCR.program Profile60C300 @19:49:27.21 Profile60C300 should complete in < 1:45:21 @19:49:27.22 PCR.run 2:16:41.25 @21:11:21.45 PCR.off @21:11:23.70 PCR for Bb completed -- closed /var/log/esp/Bb18oct0219h.pcr @21:11:23.77 Cleaning PCR @21:11:23.78 AFV.select :carrierSolution @21:11:23.89 delay 2 seconds @21:11:25.92 AS.reconfigure ASconfig @21:11:25.97 AS.seek 0.5ml @21:11:36.26 delay 1 second @21:11:37.27 AFV.select :holdingCoil @21:11:37.38 ASV.dial :waste1 @21:11:37.74 ATV.dial :air3 @21:11:38.92 AS.reconfigure ASslow1 @21:11:38.97 AS.seek 0.51ml @21:11:41.26 delay 2 seconds @21:11:43.28 ATV.dial :bleach @21:11:43.88 AS.seek 0.81ml @21:12:44.46 delay 3 seconds @21:12:47.48 ATV.dial :air3 @21:12:48.01 delay 3 seconds @21:12:51.10 AS.seek 0.835ml @21:12:56.41 delay 3 seconds @21:12:59.43 ATV.dial :bleach @21:13:00.03 AS.seek 0.82ml @21:13:03.34 delay 3 seconds @21:13:06.36 ARV.dial :waste @21:13:06.93 delay 3 seconds @21:13:09.95 ATV.dial :ARV @21:13:10.89 AS.reconfigure ASconfig @21:13:10.94 AS.seek 0.47ml @21:13:31.34 AFV.select :carrierSolution @21:13:31.46 delay 2 seconds @21:13:33.49 AS.seek 1ml @21:14:04.23 delay 1 second @21:14:05.24 AFV.select :holdingCoil @21:14:05.35 delay 1 second @21:14:06.38 AS.reconfigure ASfast @21:14:06.43 AS.seek 0ml @21:14:33.78 AFV.select :carrierSolution @21:14:33.90 delay 2 seconds @21:14:35.93 AS.reconfigure ASconfig @21:14:35.98 AS.seek 1ml @21:15:33.76 delay 1 second @21:15:34.77 AFV.select :holdingCoil @21:15:34.88 delay 1 second @21:15:35.91 AS.reconfigure ASfast @21:15:35.96 AS.seek 0ml @21:16:03.30 AFV.select :carrierSolution @21:16:03.42 delay 2 seconds @21:16:05.45 AS.reconfigure ASconfig @21:16:05.50 AS.seek 1ml @21:17:03.29 delay 1 second @21:17:04.30 AFV.select :holdingCoil @21:17:04.41 delay 1 second @21:17:05.44 AS.reconfigure ASfast @21:17:05.49 AS.seek 0ml @21:17:32.81 ARV.dial between carrier and air1 @21:17:33.29 ATV.dial :air3 @21:17:34.49 AS.reconfigure ASslow1 @21:17:34.54 AS.seek 0.01ml @21:17:36.89 delay 2 seconds @21:17:38.90 ATV.dial :bleach @21:17:39.50 AS.seek 0.81ml @21:20:20.64 delay 3 seconds @21:20:23.66 ATV.dial :air3 @21:20:24.18 delay 3 seconds @21:20:27.26 AS.seek 0.835ml @21:20:32.57 delay 3 seconds @21:20:35.59 ATV.dial :bleach @21:20:36.19 AS.seek 0.82ml @21:20:39.51 delay 3 seconds @21:20:42.53 ATV.dial PCR @21:20:42.92 AS.reconfigure ASconfig @21:20:42.97 AS.seek :empty @21:21:30.44 PCR.program :bleachProfile @21:21:30.51 PCR program should complete in < 2:45 @21:21:30.52 PCR.run 10:00 @21:24:38.40 PCR.off @21:24:38.42 AFV.select :carrierSolution @21:24:38.53 delay 2 seconds @21:24:40.56 AS.seek 1ml @21:25:38.40 delay 1 second @21:25:39.41 AFV.select :holdingCoil @21:25:39.52 delay 1 second @21:25:40.55 AS.reconfigure ASfast @21:25:40.60 AS.seek 0ml @21:26:07.95 delay 3 seconds @21:26:10.97 AFV.select :carrierSolution @21:26:11.08 delay 2 seconds @21:26:13.73 AS.reconfigure ASconfig @21:26:13.78 AS.seek 1ml @21:27:11.56 delay 1 second @21:27:12.57 AFV.select :holdingCoil @21:27:12.68 delay 1 second @21:27:13.71 AS.reconfigure ASfast @21:27:13.76 AS.seek 0ml @21:27:41.11 delay 3 seconds @21:27:44.13 AFV.select :carrierSolution @21:27:44.24 delay 2 seconds @21:27:46.27 AS.reconfigure ASconfig @21:27:46.32 AS.seek 1ml @21:28:44.06 delay 1 second @21:28:45.07 AFV.select :holdingCoil @21:28:45.18 delay 1 second @21:28:46.21 AS.reconfigure ASfast @21:28:46.26 AS.seek 0ml @21:29:13.61 delay 3 seconds @21:29:16.63 AFV.select :carrierSolution @21:29:16.74 delay 2 seconds @21:29:18.77 AS.reconfigure ASconfig @21:29:18.83 AS.seek 1ml @21:30:16.62 delay 1 second @21:30:17.63 AFV.select :holdingCoil @21:30:17.74 delay 1 second @21:30:18.77 AS.reconfigure ASfast @21:30:18.82 AS.seek 0ml @21:30:46.16 delay 3 seconds @21:30:49.18 AFV.select :carrierSolution @21:30:49.29 delay 2 seconds @21:30:51.32 AS.reconfigure ASconfig @21:30:51.37 AS.seek 1ml @21:31:49.17 delay 1 second @21:31:50.18 AFV.select :holdingCoil @21:31:50.29 delay 1 second @21:31:51.32 AS.reconfigure ASnorm @21:31:51.37 AS.seek 0ml @21:32:42.10 Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0221h.pcr @21:32:42.12 Building PCR Slug from ASV with MM1 mix, PP2 primer @21:32:42.14 Priming with carrier @21:32:42.15 ATV.dial ARV @21:32:42.72 ARV.dial :carrier @21:32:43.07 AS.seek 0.5ml @21:33:08.60 AFV.select :carrierSolution @21:33:08.72 delay 2 seconds @21:33:10.75 AS.reconfigure ASconfig @21:33:10.80 AS.seek 1ml @21:33:39.84 delay 1 second @21:33:40.85 AFV.select :holdingCoil @21:33:40.96 delay 1 second @21:33:41.98 ATV.dial :waste @21:33:43.54 AS.reconfigure ASnorm @21:33:43.64 AS.seek :empty @21:34:34.39 AFV.select :carrierSolution @21:34:34.50 delay 2 seconds @21:34:36.53 AS.reconfigure ASconfig @21:34:36.58 AS.seek 0.5ml @21:35:05.69 delay 1 second @21:35:06.70 AFV.select :holdingCoil @21:35:06.81 Building Slug @21:35:06.82 ATV.dial :ARV @21:35:08.31 delay 2 seconds @21:35:10.33 ARV.dial :air1 @21:35:10.71 delay 2 seconds @21:35:12.79 AS.reconfigure ASslow2 @21:35:12.85 AS.seek 0.506ml @21:35:19.13 delay 2 seconds @21:35:21.15 ARV.dial :MM1 @21:35:21.73 delay 2 seconds @21:35:23.76 Adding MM1 @21:35:23.83 AS.seek 0.524ml @21:35:42.14 delay 25 seconds @21:36:07.16 ARV.dial :PP2 @21:36:07.55 Adding PP2 @21:36:07.56 delay 2 seconds @21:36:09.65 AS.seek 0.53ml @21:36:16.02 delay 10 seconds @21:36:26.04 ARV.dial :air2 @21:36:26.42 adding ARV air @21:36:26.43 delay 2 seconds @21:36:28.52 AS.seek 0.536ml @21:36:35.03 delay 5 seconds @21:36:40.05 ARV.dial :carrier @21:36:41.24 delay 7 seconds @21:36:48.33 AS.seek 0.594ml @21:37:47.06 delay 5 seconds @21:37:52.08 ATV.dial :ASV @21:37:52.47 delay 2 seconds @21:37:54.56 AS.seek 0.6ml @21:38:01.05 delay 4 seconds @21:38:05.07 ATV.dial ARV @21:38:05.58 delay 2 seconds @21:38:07.60 ARV.dial :carrier @21:38:07.62 delay 2 seconds @21:38:09.71 AS.seek 0.65ml @21:39:00.19 delay 4 seconds @21:39:04.21 ATV.dial PCR @21:39:04.78 ASV.dial :waste1 @21:39:04.79 ARV.dial :air1 @21:39:05.17 Positioning Slug by 0.324ml @21:39:05.27 AS.seek 0.326ml @21:44:31.38 delay 5 seconds @21:44:36.40 ATV.dial ARV @21:44:37.74 ASV.dial :PCRlock @21:44:38.11 delay 5 seconds @21:44:43.14 Running Ss Profile60C350 thermocycling @21:44:43.15 PCR.program Profile60C350 @21:44:43.30 Profile60C350 should complete in < 1:48:05 @21:44:43.31 PCR.run 2:20:06.25 @23:07:11.77 PCR.off @23:07:14.19 PCR for Ss completed -- closed /var/log/esp/Ss18oct0221h.pcr @23:07:14.26 Cleaning PCR @23:07:14.27 AFV.select :carrierSolution @23:07:14.38 delay 2 seconds @23:07:16.41 AS.reconfigure ASconfig @23:07:16.46 AS.seek 0.5ml @23:07:26.79 delay 1 second @23:07:27.80 AFV.select :holdingCoil @23:07:27.91 ASV.dial :waste1 @23:07:28.27 ATV.dial :air3 @23:07:29.48 AS.reconfigure ASslow1 @23:07:29.54 AS.seek 0.51ml @23:07:31.84 delay 2 seconds @23:07:33.86 ATV.dial :bleach @23:07:34.56 AS.seek 0.81ml @23:08:35.15 delay 3 seconds @23:08:38.17 ATV.dial :air3 @23:08:38.70 delay 3 seconds @23:08:41.79 AS.seek 0.835ml @23:08:47.07 delay 3 seconds @23:08:50.09 ATV.dial :bleach @23:08:50.69 AS.seek 0.82ml @23:08:53.99 delay 3 seconds @23:08:57.01 ARV.dial :waste @23:08:57.60 delay 3 seconds @23:09:00.62 ATV.dial :ARV @23:09:01.46 AS.reconfigure ASconfig @23:09:01.52 AS.seek 0.47ml @23:09:21.92 AFV.select :carrierSolution @23:09:22.04 delay 2 seconds @23:09:24.07 AS.seek 1ml @23:09:54.89 delay 1 second @23:09:55.91 AFV.select :holdingCoil @23:09:56.02 delay 1 second @23:09:57.05 AS.reconfigure ASfast @23:09:57.10 AS.seek 0ml @23:10:24.45 AFV.select :carrierSolution @23:10:24.57 delay 2 seconds @23:10:26.60 AS.reconfigure ASconfig @23:10:26.65 AS.seek 1ml @23:11:24.42 delay 1 second @23:11:25.44 AFV.select :holdingCoil @23:11:25.55 delay 1 second @23:11:26.58 AS.reconfigure ASfast @23:11:26.63 AS.seek 0ml @23:11:53.95 AFV.select :carrierSolution @23:11:54.07 delay 2 seconds @23:11:56.10 AS.reconfigure ASconfig @23:11:56.15 AS.seek 1ml @23:12:53.92 delay 1 second @23:12:54.94 AFV.select :holdingCoil @23:12:55.05 delay 1 second @23:12:56.08 AS.reconfigure ASfast @23:12:56.13 AS.seek 0ml @23:13:23.47 ARV.dial between carrier and air1 @23:13:23.97 ATV.dial :air3 @23:13:25.17 AS.reconfigure ASslow1 @23:13:25.23 AS.seek 0.01ml @23:13:27.56 delay 2 seconds @23:13:29.57 ATV.dial :bleach @23:13:30.29 AS.seek 0.81ml @23:16:11.42 delay 3 seconds @23:16:14.44 ATV.dial :air3 @23:16:14.97 delay 3 seconds @23:16:18.05 AS.seek 0.835ml @23:16:23.38 delay 3 seconds @23:16:26.40 ATV.dial :bleach @23:16:27.00 AS.seek 0.82ml @23:16:30.28 delay 3 seconds @23:16:33.31 ATV.dial PCR @23:16:33.70 AS.reconfigure ASconfig @23:16:33.75 AS.seek :empty @23:17:21.30 PCR.program :bleachProfile @23:17:21.36 PCR program should complete in < 2:45 @23:17:21.37 PCR.run 10:00 @23:20:29.27 PCR.off @23:20:29.29 AFV.select :carrierSolution @23:20:29.40 delay 2 seconds @23:20:32.05 AS.seek 1ml @23:21:29.88 delay 1 second @23:21:30.90 AFV.select :holdingCoil @23:21:31.01 delay 1 second @23:21:32.04 AS.reconfigure ASfast @23:21:32.09 AS.seek 0ml @23:21:59.45 delay 3 seconds @23:22:02.47 AFV.select :carrierSolution @23:22:02.58 delay 2 seconds @23:22:04.61 AS.reconfigure ASconfig @23:22:04.66 AS.seek 1ml @23:23:02.44 delay 1 second @23:23:03.46 AFV.select :holdingCoil @23:23:03.57 delay 1 second @23:23:04.60 AS.reconfigure ASfast @23:23:04.65 AS.seek 0ml @23:23:31.98 delay 3 seconds @23:23:35.00 AFV.select :carrierSolution @23:23:35.11 delay 2 seconds @23:23:37.14 AS.reconfigure ASconfig @23:23:37.20 AS.seek 1ml @23:24:35.05 delay 1 second @23:24:36.06 AFV.select :holdingCoil @23:24:36.17 delay 1 second @23:24:37.20 AS.reconfigure ASfast @23:24:37.25 AS.seek 0ml @23:25:04.61 delay 3 seconds @23:25:07.63 AFV.select :carrierSolution @23:25:07.74 delay 2 seconds @23:25:09.77 AS.reconfigure ASconfig @23:25:09.82 AS.seek 1ml @23:26:07.62 delay 1 second @23:26:08.64 AFV.select :holdingCoil @23:26:08.75 delay 1 second @23:26:09.78 AS.reconfigure ASfast @23:26:09.83 AS.seek 0ml @23:26:37.16 delay 3 seconds @23:26:40.17 AFV.select :carrierSolution @23:26:40.28 delay 2 seconds @23:26:42.31 AS.reconfigure ASconfig @23:26:42.36 AS.seek 1ml @23:27:40.17 delay 1 second @23:27:41.18 AFV.select :holdingCoil @23:27:41.29 delay 1 second @23:27:42.32 AS.reconfigure ASnorm @23:27:42.37 AS.seek 0ml @23:28:33.07 Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0223h.pcr @23:28:33.09 Building PCR Slug from ASV with MM1 mix, PP1 primer @23:28:33.10 Priming with carrier @23:28:33.11 ATV.dial ARV @23:28:33.68 ARV.dial :carrier @23:28:34.04 AS.seek 0.5ml @23:28:59.54 AFV.select :carrierSolution @23:28:59.66 delay 2 seconds @23:29:01.69 AS.reconfigure ASconfig @23:29:01.74 AS.seek 1ml @23:29:30.81 delay 1 second @23:29:31.83 AFV.select :holdingCoil @23:29:31.94 delay 1 second @23:29:32.96 ATV.dial :waste @23:29:34.49 AS.reconfigure ASnorm @23:29:34.54 AS.seek :empty @23:30:25.27 AFV.select :carrierSolution @23:30:25.39 delay 2 seconds @23:30:27.42 AS.reconfigure ASconfig @23:30:27.47 AS.seek 0.5ml @23:30:56.58 delay 1 second @23:30:57.59 AFV.select :holdingCoil @23:30:57.70 Building Slug @23:30:57.71 ATV.dial :ARV @23:30:59.20 delay 2 seconds @23:31:01.22 ARV.dial :air1 @23:31:01.71 delay 2 seconds @23:31:03.79 AS.reconfigure ASslow2 @23:31:03.84 AS.seek 0.506ml @23:31:10.10 delay 2 seconds @23:31:12.12 ARV.dial :MM1 @23:31:12.80 delay 2 seconds @23:31:14.83 Adding MM1 @23:31:14.90 AS.seek 0.524ml @23:31:33.31 delay 25 seconds @23:31:58.33 ARV.dial :PP1 @23:31:58.72 Adding PP1 @23:31:58.73 delay 2 seconds @23:32:00.82 AS.seek 0.53ml @23:32:07.08 delay 10 seconds @23:32:17.10 ARV.dial :air1 @23:32:17.47 adding ARV air @23:32:17.48 delay 2 seconds @23:32:19.56 AS.seek 0.536ml @23:32:25.79 delay 5 seconds @23:32:30.82 ARV.dial :carrier @23:32:31.22 delay 7 seconds @23:32:38.31 AS.seek 0.594ml @23:33:37.04 delay 5 seconds @23:33:42.07 ATV.dial :ASV @23:33:42.60 delay 2 seconds @23:33:44.68 AS.seek 0.6ml @23:33:50.98 delay 4 seconds @23:33:55.01 ATV.dial ARV @23:33:55.52 delay 2 seconds @23:33:57.54 ARV.dial :carrier @23:33:57.56 delay 2 seconds @23:33:59.65 AS.seek 0.65ml @23:34:50.03 delay 4 seconds @23:34:54.05 ATV.dial PCR @23:34:54.63 ASV.dial :waste1 @23:34:54.64 ARV.dial :air1 @23:34:55.64 Positioning Slug by 0.324ml @23:34:55.73 AS.seek 0.326ml @23:40:21.74 delay 5 seconds @23:40:26.76 ATV.dial ARV @23:40:27.48 ASV.dial :PCRlock @23:40:27.86 delay 5 seconds @23:40:32.89 Running IPc ProfileIPc thermocycling @23:40:32.90 PCR.program ProfileIPc @23:40:33.05 ProfileIPc should complete in < 1:50:49 @23:40:33.06 PCR.run 2:23:31.25 @01:04:48.13CEST03-Oct-18 PCR.off @01:04:50.85 PCR for IPc completed -- closed /var/log/esp/IPc18oct0223h.pcr @01:04:50.86 Full MFB Decontamination @01:04:50.87 Decon extraction column and decon AIV @01:04:50.90 SPE.hold 2:46:40,55C @01:04:50.94 ASV.dial :SPEdelivery @01:04:51.62 delay 20 seconds @01:05:11.64 AFV.select :carrierSolution @01:05:11.75 delay 2 seconds @01:05:13.78 AS.reconfigure ASconfig @01:05:13.83 AS.seek 0.5ml @01:05:24.14 delay 1 second @01:05:25.15 AFV.select :holdingCoil @01:05:25.26 ATV.dial :air1 @01:05:26.90 AS.reconfigure ASslow1 @01:05:26.96 AS.seek 0.52ml @01:05:31.24 ATV.dial :ASV @01:05:32.77 ASV.dial :SPErecovery @01:05:33.41 AS.seek 0.62ml @01:05:53.77 ATV.dial :waste @01:05:55.36 AS.reconfigure ASnorm @01:05:55.41 AS.seek :empty @01:06:27.02 AFV.select :holdingCoil @01:06:27.04 ATV.dial :waste @01:06:27.06 AS.reconfigure ASconfig @01:06:27.10 AS.seek 0ml @01:06:27.39 AFV.select :carrierSolution @01:06:27.51 delay 2 seconds @01:06:29.54 AS.seek 0.5ml @01:06:58.58 delay 1 second @01:06:59.59 AFV.select :holdingCoil @01:06:59.71 ATV.dial :air3 @01:07:00.48 AS.reconfigure ASslow1 @01:07:00.53 AS.seek 0.52ml @01:07:04.82 ATV.dial :air3 @01:07:04.91 AS.seek 0.53ml @01:07:07.15 delay 2 seconds @01:07:09.16 ATV.dial :bleach @01:07:09.61 AS.seek 0.71ml @01:07:46.00 delay 3 seconds @01:07:49.02 ATV.dial :air3 @01:07:49.54 delay 3 seconds @01:07:52.62 AS.seek 0.735ml @01:07:57.93 delay 3 seconds @01:08:00.95 ATV.dial :bleach @01:08:01.55 AS.seek 0.72ml @01:08:04.89 delay 3 seconds @01:08:07.92 ATV.dial ASV @01:08:08.87 ASV.dial :SPErecovery @01:08:08.95 AS.seek 0.365ml @01:09:20.60 ATV.dial :waste @01:09:22.28 AS.reconfigure ASnorm @01:09:22.33 AS.seek :empty @01:09:41.11 ATV.dial :air3 @01:09:41.85 AS.reconfigure ASslow1 @01:09:41.91 AS.seek 0.009ml @01:09:44.79 delay 2 seconds @01:09:46.81 ATV.dial :bleach @01:09:47.29 AS.seek 0.579ml @01:11:42.19 delay 3 seconds @01:11:45.21 ATV.dial :air3 @01:11:45.75 delay 3 seconds @01:11:48.84 AS.seek 0.604ml @01:11:54.14 delay 3 seconds @01:11:57.16 ATV.dial :bleach @01:11:57.76 AS.seek 0.589ml @01:12:01.08 delay 3 seconds @01:12:04.10 ATV.dial :ASV @01:12:05.06 ASV.dial :wasteR @01:12:05.53 AS.seek 0ml @01:14:04.26 delay 1:00 @01:15:04.28 ATV.dial ASV @01:15:04.30 AFV.select :carrierSolution @01:15:04.40 delay 2 seconds @01:15:06.43 AS.reconfigure ASconfig @01:15:06.48 AS.seek 1ml @01:16:04.25 delay 1 second @01:16:05.26 AFV.select :holdingCoil @01:16:05.37 delay 1 second @01:16:06.39 ASV.dial :wasteR @01:16:06.41 AS.reconfigure ASslow1 @01:16:06.46 AS.seek 0ml @01:19:27.84 delay 10 seconds @01:19:37.86 ATV.dial :air3 @01:19:39.26 AS.seek 0.01ml @01:19:41.56 delay 2 seconds @01:19:43.57 ATV.dial :bleach @01:19:44.16 AS.seek 0.58ml @01:21:39.11 delay 3 seconds @01:21:42.13 ATV.dial :air3 @01:21:42.66 delay 3 seconds @01:21:45.74 AS.seek 0.605ml @01:21:51.05 delay 3 seconds @01:21:54.07 ATV.dial :bleach @01:21:54.66 AS.seek 0.59ml @01:21:57.99 delay 3 seconds @01:22:01.01 ATV.dial ASV @01:22:02.07 ASV.dial :SPEdelivery @01:22:02.44 AS.seek 0ml @01:24:01.39 delay 1:00 @01:25:01.41 AFV.select :carrierSolution @01:25:01.52 delay 2 seconds @01:25:03.55 AS.reconfigure ASconfig @01:25:03.60 AS.seek 1ml @01:26:01.35 delay 1 second @01:26:02.36 AFV.select :holdingCoil @01:26:02.47 delay 1 second @01:26:03.49 ATV.dial ASV @01:26:03.51 ASV.dial :SPEdelivery @01:26:03.53 AS.reconfigure ASslow1 @01:26:03.57 AS.seek 0ml @01:29:24.96 delay 10 seconds @01:29:34.98 ATV.dial :air2 @01:29:35.77 AS.reconfigure ASslow0 @01:29:35.82 AS.seek 0.8ml @01:30:33.43 Session Begun @01:30:56.63 delay 3 seconds @01:30:59.65 ATV.dial :ASV @01:31:00.69 ASV.dial :SPEdelivery @01:31:00.72 AS.reconfigure ASslow1 @01:31:00.81 AS.seek 0.03ml @01:31:12.72 -> GoodNews.recipients @01:33:34.31 -> showlog 50 @01:33:35.97 delay 20 seconds @01:33:52.38 -> quit @01:33:52.40 Session Ended @01:33:56.00 ATV.dial :waste,via: :air3 @01:33:57.61 AS.reconfigure ASconfig @01:33:57.66 AS.seek :empty @01:33:59.73 SPE.coast @01:33:59.82 Removing Bleach from Mixing Coil @01:33:59.83 AFV.select :carrierSolution @01:33:59.94 delay 2 seconds @01:34:01.97 AS.seek 0.5ml @01:34:31.08 delay 1 second @01:34:32.09 AFV.select :holdingCoil @01:34:32.20 ATV.dial :ASV @01:34:33.85 ASV.dial :SPErecovery @01:34:34.47 AS.reconfigure ASslow1 @01:34:34.53 AS.seek 0.73ml @01:35:21.06 ASV.dial :wasteD @01:35:21.61 AS.reconfigure ASnorm @01:35:21.66 AS.seek 0ml @01:35:58.78 AFV.select :carrierSolution @01:35:58.90 delay 2 seconds @01:36:00.93 AS.reconfigure ASconfig @01:36:00.98 AS.seek 0.5ml @01:36:30.03 delay 1 second @01:36:31.05 AFV.select :holdingCoil @01:36:31.16 ATV.dial ASV @01:36:31.17 ASV.dial :SPErecovery @01:36:31.78 AS.reconfigure ASslow0 @01:36:31.84 AS.seek 0.3ml @01:36:52.87 ATV.dial :air2 @01:36:53.95 AS.reconfigure ASslow1 @01:36:54.00 AS.seek 0.32ml @01:36:58.35 ATV.dial ASV @01:36:59.32 AS.reconfigure ASslow0 @01:36:59.37 AS.seek 0.57ml @01:37:24.81 ASV.dial :wasteD @01:37:25.38 AS.reconfigure ASnorm @01:37:25.43 AS.seek 0ml @01:37:54.48 AFV.select :carrierSolution @01:37:54.60 delay 2 seconds @01:37:56.63 AS.reconfigure ASconfig @01:37:56.68 AS.seek 0.5ml @01:38:25.79 delay 1 second @01:38:26.80 AFV.select :holdingCoil @01:38:26.91 ATV.dial ASV @01:38:26.92 ASV.dial :SPErecovery @01:38:27.54 AS.reconfigure ASslow0 @01:38:27.59 AS.seek 0.3ml @01:38:48.03 ATV.dial :air2 @01:38:49.12 AS.reconfigure ASslow1 @01:38:49.17 AS.seek 0.32ml @01:38:53.48 ATV.dial ASV @01:38:54.45 AS.reconfigure ASslow0 @01:38:54.50 AS.seek 0.57ml @01:39:19.97 ASV.dial :wasteD @01:39:20.54 AS.reconfigure ASnorm @01:39:20.59 AS.seek 0ml @01:39:49.67 ATV.dial :air2 @01:39:50.60 AS.reconfigure ASslow0 @01:39:50.70 AS.seek 0.3ml @01:40:21.28 ATV.dial :ASV @01:40:22.20 ASV.dial :wasteD @01:40:22.23 AS.reconfigure ASconfig @01:40:22.27 AS.seek 0.05ml @01:40:36.96 ATV.dial :waste @01:40:38.77 AS.seek 0ml @01:40:41.95 ASV.dial :SPEdelivery @01:40:43.00 deconLysateINT @01:40:43.04 ATV.dial :air3 @01:40:43.88 AS.reconfigure ASslow1 @01:40:43.94 AS.seek 0.01ml @01:40:46.33 delay 2 seconds @01:40:48.34 ATV.dial :bleach @01:40:48.79 AS.seek 0.96ml @01:44:00.18 delay 3 seconds @01:44:03.20 ATV.dial :air3 @01:44:03.72 delay 3 seconds @01:44:06.81 AS.seek 0.985ml @01:44:12.07 delay 3 seconds @01:44:15.09 ATV.dial :bleach @01:44:15.69 AS.seek 0.97ml @01:44:18.99 delay 3 seconds @01:44:22.02 ATV.dial AIV @01:44:23.27 AIV.dial :coilToMFB @01:44:23.28 AWV.select :waste @01:44:23.29 AFV.select :carrierSolution @01:44:23.41 delay 2 seconds @01:44:25.44 AS.reconfigure ASconfig @01:44:25.49 AS.seek 1ml @01:44:27.52 delay 1 second @01:44:28.53 AFV.select :holdingCoil @01:44:28.64 delay 1 second @01:44:29.67 AS.reconfigure ASslow0 @01:44:29.72 AS.seek :empty @01:46:10.65 AFV.select :carrierSolution @01:46:10.77 delay 2 seconds @01:46:12.80 AS.reconfigure ASconfig @01:46:12.85 AS.seek 1ml @01:47:10.73 delay 1 second @01:47:11.74 AFV.select :holdingCoil @01:47:11.85 delay 1 second @01:47:12.88 AS.reconfigure ASslow0 @01:47:12.92 AS.seek :empty @01:48:53.90 AFV.select :carrierSolution @01:48:54.02 delay 2 seconds @01:48:56.05 AS.reconfigure ASconfig @01:48:56.10 AS.seek 1ml @01:49:53.98 delay 1 second @01:49:54.99 AFV.select :holdingCoil @01:49:55.10 delay 1 second @01:49:56.13 AS.reconfigure ASslow0 @01:49:56.17 AS.seek :empty @01:51:37.14 ATV.dial :air2 @01:51:37.79 AS.reconfigure ASslow1 @01:51:37.84 AS.seek 0.009ml @01:51:40.16 AS.reconfigure ASslow0 @01:51:40.21 AS.seek 1ml @01:53:20.14 ATV.dial AIV @01:53:21.57 AS.seek 0.02ml @01:55:00.47 ATV.dial :waste @01:55:01.98 AS.seek 0ml @01:55:04.29 AWV.select :air @01:55:04.46 Cleaning PCR @01:55:04.48 AFV.select :carrierSolution @01:55:04.58 delay 2 seconds @01:55:06.61 AS.reconfigure ASconfig @01:55:06.66 AS.seek 0.5ml @01:55:35.70 delay 1 second @01:55:36.71 AFV.select :holdingCoil @01:55:36.82 ASV.dial :waste1 @01:55:37.16 ATV.dial :air3 @01:55:37.90 AS.reconfigure ASslow1 @01:55:37.96 AS.seek 0.51ml @01:55:40.32 delay 2 seconds @01:55:42.33 ATV.dial :bleach @01:55:42.80 AS.seek 0.81ml @01:56:43.40 delay 3 seconds @01:56:46.42 ATV.dial :air3 @01:56:46.95 delay 3 seconds @01:56:50.06 AS.seek 0.835ml @01:56:55.41 delay 3 seconds @01:56:58.43 ATV.dial :bleach @01:56:59.03 AS.seek 0.82ml @01:57:02.29 delay 3 seconds @01:57:05.31 ARV.dial :waste @01:57:05.90 delay 3 seconds @01:57:08.92 ATV.dial :ARV @01:57:09.85 AS.reconfigure ASconfig @01:57:09.90 AS.seek 0.47ml @01:57:30.30 AFV.select :carrierSolution @01:57:30.42 delay 2 seconds @01:57:32.45 AS.seek 1ml @01:58:03.23 delay 1 second @01:58:04.24 AFV.select :holdingCoil @01:58:04.35 delay 1 second @01:58:05.38 AS.reconfigure ASfast @01:58:05.43 AS.seek 0ml @01:58:32.78 AFV.select :carrierSolution @01:58:32.90 delay 2 seconds @01:58:34.93 AS.reconfigure ASconfig @01:58:34.98 AS.seek 1ml @01:59:32.76 delay 1 second @01:59:33.77 AFV.select :holdingCoil @01:59:33.88 delay 1 second @01:59:34.91 AS.reconfigure ASfast @01:59:34.96 AS.seek 0ml @02:00:02.31 AFV.select :carrierSolution @02:00:02.43 delay 2 seconds @02:00:04.46 AS.reconfigure ASconfig @02:00:04.51 AS.seek 1ml @02:01:02.29 delay 1 second @02:01:03.30 AFV.select :holdingCoil @02:01:03.41 delay 1 second @02:01:04.44 AS.reconfigure ASfast @02:01:04.49 AS.seek 0ml @02:01:31.83 ARV.dial between carrier and air1 @02:01:32.32 ATV.dial :air3 @02:01:33.51 AS.reconfigure ASslow1 @02:01:33.56 AS.seek 0.01ml @02:01:35.93 delay 2 seconds @02:01:37.94 ATV.dial :bleach @02:01:38.55 AS.seek 0.81ml @02:04:19.70 delay 3 seconds @02:04:22.72 ATV.dial :air3 @02:04:23.24 delay 3 seconds @02:04:26.32 AS.seek 0.835ml @02:04:31.63 delay 3 seconds @02:04:34.65 ATV.dial :bleach @02:04:35.25 AS.seek 0.82ml @02:04:38.53 delay 3 seconds @02:04:41.56 ATV.dial PCR @02:04:41.95 AS.reconfigure ASconfig @02:04:42.00 AS.seek :empty @02:05:29.45 PCR.program :bleachProfile @02:05:29.52 PCR program should complete in < 2:45 @02:05:29.53 PCR.run 10:00 @02:08:49.53 PCR.off @02:08:49.56 AFV.select :carrierSolution @02:08:49.68 delay 2 seconds @02:08:52.33 AS.seek 1ml @02:09:50.15 delay 1 second @02:09:51.16 AFV.select :holdingCoil @02:09:51.27 delay 1 second @02:09:52.30 AS.reconfigure ASfast @02:09:52.35 AS.seek 0ml @02:10:19.68 delay 3 seconds @02:10:22.70 AFV.select :carrierSolution @02:10:22.81 delay 2 seconds @02:10:24.84 AS.reconfigure ASconfig @02:10:24.89 AS.seek 1ml @02:11:22.67 delay 1 second @02:11:23.68 AFV.select :holdingCoil @02:11:23.80 delay 1 second @02:11:24.84 AS.reconfigure ASfast @02:11:24.89 AS.seek 0ml @02:11:52.23 delay 3 seconds @02:11:55.25 AFV.select :carrierSolution @02:11:55.36 delay 2 seconds @02:11:57.39 AS.reconfigure ASconfig @02:11:57.45 AS.seek 1ml @02:12:55.23 delay 1 second @02:12:56.24 AFV.select :holdingCoil @02:12:56.35 delay 1 second @02:12:57.38 AS.reconfigure ASfast @02:12:57.43 AS.seek 0ml @02:13:24.79 delay 3 seconds @02:13:27.81 AFV.select :carrierSolution @02:13:27.92 delay 2 seconds @02:13:29.96 AS.reconfigure ASconfig @02:13:30.00 AS.seek 1ml @02:14:27.78 delay 1 second @02:14:28.79 AFV.select :holdingCoil @02:14:28.90 delay 1 second @02:14:29.93 AS.reconfigure ASfast @02:14:29.98 AS.seek 0ml @02:14:57.33 delay 3 seconds @02:15:00.35 AFV.select :carrierSolution @02:15:00.46 delay 2 seconds @02:15:02.49 AS.reconfigure ASconfig @02:15:02.55 AS.seek 1ml @02:16:00.33 delay 1 second @02:16:01.34 AFV.select :holdingCoil @02:16:01.45 delay 1 second @02:16:02.48 AS.reconfigure ASnorm @02:16:02.53 AS.seek 0ml @02:16:53.24 AFV.select :carrierSolution @02:16:53.35 delay 2 seconds @02:16:55.38 AS.reconfigure ASconfig @02:16:55.43 AS.seek 1ml @02:17:53.23 delay 1 second @02:17:54.24 AFV.select :holdingCoil @02:17:54.35 delay 1 second @02:17:55.37 ATV.dial :waste @02:17:56.51 AS.reconfigure ASnorm @02:17:56.56 AS.seek 0.5ml @02:18:22.11 ATV.dial :waste2 @02:18:23.28 AS.seek 0ml @02:18:48.81 AS.coast @02:18:48.85 SS.coast @02:18:48.89 CC.coast @02:18:48.93 CS.coast @02:18:48.97 PC.coast @02:18:49.01 PS.coast @02:18:49.05 Forearm.coast @02:18:49.09 Elbow.coast @02:18:49.49 Gate.power :core,:OFF @02:18:49.50 Gate.power :raw,:OFF @02:18:49.51 Shutdown @02:18:49.53 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @02:19:29.64 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @02:19:29.65 Retry #1 of 10 after 10:00 @02:29:29.68 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @14:00:00.08
Powering core bus @14:00:04.81 Gate.power :raw,:ON @14:00:06.04 SPE.coast @14:00:06.11 SPE.configure SPEconfig @14:00:06.21 AS.configure ASconfig @14:00:06.31 SS.configure SSconfig @14:00:06.41 CC.configure CCconfig @14:00:06.51 CS.configure CSconfig @14:00:06.61 PC.configure PCconfig @14:00:06.71 PS.configure PSconfig @14:00:06.83 Forearm.configure LooseForearm @14:00:06.93 Elbow.configure LooseElbow @14:00:07.05 SC.configure SCloose @14:00:07.15 SE.configure SEconfig @14:00:07.20 CH.coast @14:00:07.26 CH.configure CHconfig @14:00:07.29 PH.coast @14:00:07.35 PH.configure PHconfig @14:00:07.40 PCR.off @14:00:07.43 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:07.45 PCR.caltemp= 2.7073,0.0373,0 @14:00:07.48 All dwarf controllers running firmware version 3.75 @14:00:07.61 Valve.reset @14:00:07.78 PCR.off @14:00:07.81 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:07.84 PCR.caltemp= 2.7073,0.0373,0 @14:00:08.00 Intake.select :close @14:00:08.03 Exhaust.select :close @14:00:08.06 CTV.dial :air @14:00:08.19 PSV.dial :air @14:00:08.23 CSV.dial :waste1 @14:00:08.41 SSV.dial :spit,avoiding: :exhaust @14:00:08.43
Forearm.reconfigure LooseForearm @14:00:08.55 Forearm.home.seek :retract @14:00:08.72 PS.reconfigure PSconfig @14:00:08.77 PS.home.seek 0ml @14:00:09.36 Hand.close @14:00:09.42 CS.reconfigure CSfast @14:00:09.44
Elbow.jog 1500 @14:00:09.47 SS.reconfigure SSconfig @14:00:09.67 CS.home.seek 0ml @14:00:09.74 SS.home.seek 0ml @14:00:10.17
Elbow.reconfigure LooseElbow @14:00:10.21 Elbow.home.seek :clear @14:00:13.61 SE.reconfigure SEconfig @14:00:13.66 SE.home.seek :down @14:00:14.32 SC.reconfigure SCsloppy @14:00:14.36 SC.home.seek :initial @14:00:16.54 Forearm.seek :retract @14:00:16.62 Elbow.seek :hammer @14:00:17.79 PS.coast @14:00:17.90
Forearm.reconfigure TightForearm @14:00:17.95 Forearm.seek 5 + 350 counts @14:00:19.03 Forearm.seek :retract @14:00:20.23 CC.reconfigure CCconfig @14:00:20.25 PC.reconfigure PCconfig @14:00:20.33
CC.home.seek :open @14:00:20.37 PC.home.seek :open @14:00:23.85
Phase [mbaPhase] @14:00:23.87 GoodNews.email "At 14:00:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tundeployv2.rb:115\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "Started 18tundeployv2 phase mbaPhase" @14:00:23.89CEST03-Oct-18
==================> STARTED Superphase (phase #3) <=== @14:00:23.91 Starting initialPurge @14:00:23.93 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:01:04.05 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:01:04.08 Retry #1 of 10 after 10:00 @14:01:04.15
Hand.open @14:01:04.86 Elbow.reconfigure TightElbow @14:01:04.91 Elbow.seek FlushPuck::Garage @14:01:06.74 Elbow.reconfigure LooseElbow @14:01:06.85 Forearm.reconfigure LooseForearm @14:01:06.90 Forearm.seek :garage @14:01:07.86 Hand.close @14:01:08.61 Forearm.seek :retract @14:01:09.60 Elbow.reconfigure TightElbow @14:01:09.65 Elbow.seek CC @14:01:12.59 Elbow.reconfigure LooseElbow @14:01:12.70 Forearm.reconfigure TightForearm @14:01:12.75 Forearm.seek CC @14:01:14.02 CC.seek :closed @14:01:18.26 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.75 @14:01:18.35 Hand.open @14:01:19.04 Forearm.reconfigure LooseForearm @14:01:19.09 Forearm.seek :retract @14:01:20.04 Flushing the Collection Valve::Manifold @14:01:20.17 CSV.dial CSR @14:01:21.49 Pulling 5ml of CSR.flush @14:01:21.50 CSR.select :flush @14:01:21.62 CS.reconfigure CSconfig @14:01:21.66 CS.seek 5ml @14:01:35.40 delay 5 seconds @14:01:40.42 CSR.select :air @14:01:40.60 CS.reconfigure CSfast @14:01:40.65 CS.seek 7.5ml @14:01:47.14 CSV.dial :waste @14:01:48.42 CS.reconfigure CSconfig @14:01:48.47 CS.seek 0ml @14:02:08.56 Purging external Intake @14:02:08.69 CSV.dial :flush @14:02:09.15 CS.reconfigure CSfast @14:02:09.20 CS.seek 10ml @14:02:31.72 delay 5 seconds @14:02:36.75 CSV.dial CBV @14:02:36.78
CBV.dial CSV @14:02:37.72 CTV.dial :intake @14:02:38.51 Intake.select :open @14:02:38.68 CS.seek 0ml @14:03:02.02 Intake.select :close @14:03:02.14 CTV.dial :air @14:03:02.89 Flushing upper manifold @14:03:03.02 CSV.dial :flush @14:03:03.73 CS.seek 5ml @14:03:15.59 delay 5 seconds @14:03:20.61 CSV.dial :air @14:03:21.13 CS.seek 7ml @14:03:26.53 CSV.dial CBV @14:03:26.55
CBV.dial CSV @14:03:27.58 CTV.dial :air @14:03:27.60 CS.seek 0ml @14:03:43.65 delay 7 seconds @14:03:50.68 CS.seek 3ml @14:03:58.18 CSV.dial :waste @14:03:58.65 CS.seek 0ml @14:04:06.24 CSV.dial :flush @14:04:07.23 CS.seek 3ml @14:04:14.72 delay 5 seconds @14:04:19.74 CSV.dial :air @14:04:20.27 CS.seek 5ml @14:04:25.77 CSV.dial :air @14:04:25.80 CS.seek 0ml @14:04:37.62 delay 7 seconds @14:04:44.66 CS.seek 3ml @14:04:52.18 CSV.dial :waste @14:04:52.93 CS.seek 0ml @14:05:00.47 Garaging FlushPuck in Collection Clamp @14:05:00.55 Elbow.reconfigure TightElbow @14:05:00.60 Elbow.seek CC @14:05:00.86 Elbow.reconfigure LooseElbow @14:05:00.97 Forearm.seek CC @14:05:01.97 Hand.close @14:05:02.61 CC.seek :open @14:05:06.94 Forearm.seek :retract @14:05:07.91 Elbow.reconfigure TightElbow @14:05:07.96 Elbow.seek :garage @14:05:11.00 Elbow.reconfigure LooseElbow @14:05:11.11 Forearm.reconfigure TightForearm @14:05:11.15 Forearm.seek :garage @14:05:12.30 Hand.open @14:05:13.06 Forearm.reconfigure LooseForearm @14:05:13.10 Forearm.seek :retract @14:05:14.19 Starting mfb @14:05:14.20 pcrBAC sampling at most 1500ml, wcr at most 1500ml @14:05:14.25 Loading sh1 puck from tube 2 into Collection Clamp @14:05:14.40 Readying the MFB @14:05:14.46 Homing MFB Syringe @14:05:14.48 AFV.select :holdingCoil @14:05:14.74 ATV.dial :air2 @14:05:14.83
SC.reconfigure SCloose @14:05:15.06 Elbow.reconfigure TightElbow @14:05:15.11 Elbow.seek 2 @14:05:15.29 AS.reconfigure ASconfig @14:05:15.34 AS.home.seek :home @14:05:17.19
Forearm.reconfigure TightForearm @14:05:17.24 Forearm.seek 2 + 700 counts @14:05:18.29 SE.reconfigure SEpick @14:05:18.36 SE.seek beamBreak @14:05:20.20 SE.configure SEconfig @14:05:20.37 Elbow.seek 2 @14:05:20.67 Forearm.seek 2 + 300 counts @14:05:20.90 AFV.select :carrierSolution @14:05:20.96
Forearm.reconfigure LooseForearm @14:05:21.01 Hand.close @14:05:21.05 delay 2 seconds @14:05:21.63 SE.seek :down @14:05:21.86
Forearm.seek :retract @14:05:22.83 Elbow.seek CC @14:05:23.08 AS.seek 1ml @14:05:23.31 SE.coast @14:05:24.45
Elbow.reconfigure LooseElbow @14:05:24.55 Forearm.reconfigure TightForearm @14:05:24.60 Forearm.seek CC @14:05:26.44 CC.seek :closed @14:05:30.66 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.82 @14:05:30.67 Hand.open @14:05:31.43 Forearm.reconfigure LooseForearm @14:05:31.47 Forearm.seek :retract @14:05:32.46 SC.coast @14:05:32.51 collectSample 1500ml w/bubble point of 55psi @14:05:32.52 Priming 7ml @14:05:32.53 Intake.select :close @14:05:32.54 Exhaust.select :close @14:05:32.55 SS.hold @14:05:32.78 Calibrating pressure and temperature sensors @14:05:32.80 CTV.dial :air @14:05:32.81 SSV.dial :spit,avoiding: :exhaust @14:05:32.83 SS.reconfigure SSfast @14:05:32.88 SS.seek 5ml @14:05:35.45 SSV.dial CBV @14:05:35.48
CBV.dial SSV @14:05:36.86 delay 3.8 seconds @14:05:40.82 SSV.dial :spit @14:05:42.12 SS.seek 0ml @14:05:44.71 SSV.dial CBV @14:05:46.03 CTV.dial :intake @14:05:46.76 SSV.dial CBV @14:05:46.79
CBV.dial SSV @14:05:46.81 Allowing 40s for 7.0ml of remaining 7.0ml @14:05:46.82 Intake.select :open @14:05:48.99 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:05:49.01 SS.reconfigure SSinhale.with maxSpeed: 150 @14:05:49.06 SS.seek 7ml @14:05:52.16 Vacuum too high -- waiting up to 40s @14:05:53.49 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:05:53.51 SS.reconfigure SSinhale.with maxSpeed: 135 @14:05:53.56 SS.seek 7ml @14:05:56.23 Vacuum too high -- waiting up to 40s @14:05:57.83 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:05:57.85 SS.reconfigure SSinhale.with maxSpeed: 122 @14:05:57.90 SS.seek 7ml @14:06:00.17 Stroke completed -- waiting for pressure to equalize ... @14:06:01.66 Intake.select :close @14:06:01.82 SSV.dial :exhaust @14:06:02.62 Exhaust.select :open @14:06:02.78 SS.reconfigure SSexhale @14:06:02.88 SS.seek 0ml @14:06:06.41 Exhaust.select :close @14:06:06.53 Sampling 1500.0ml @14:06:06.54 Intake.select :close @14:06:06.55 Exhaust.select :close @14:06:06.57 SS.reconfigure SSconfig @14:06:06.61 SS.hold @14:06:06.83 CTV.dial :intake @14:06:07.41 SSV.dial CBV @14:06:07.47
CBV.dial SSV @14:06:08.22 Allowing 130s for 25.0ml of remaining 1500.0ml @14:06:08.23 Intake.select :open @14:06:10.50 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:06:10.53 SS.reconfigure SSinhale.with maxSpeed: 122 @14:06:10.63 SS.seek 25ml @14:06:14.38 Vacuum too high -- waiting up to 40s @14:06:15.74 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:06:15.76 SS.reconfigure SSinhale.with maxSpeed: 110 @14:06:15.81 SS.seek 25ml @14:06:16.03 delay 1 second @14:06:17.04 AFV.select :holdingCoil @14:06:17.15 delay 1 second @14:06:18.18 ATV.dial :waste @14:06:18.29
Vacuum too high -- waiting up to 40s @14:06:19.15 AS.reconfigure ASnorm @14:06:19.20 AS.home.seek :empty @14:06:19.69
Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:06:19.71 SS.reconfigure SSinhale.with maxSpeed: 99 @14:06:19.76 SS.seek 25ml @14:06:22.61 Vacuum too high -- waiting up to 40s @14:06:23.85 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:06:23.87 SS.reconfigure SSinhale.with maxSpeed: 89 @14:06:23.92 SS.seek 25ml @14:06:26.85 Vacuum too high -- waiting up to 40s @14:06:28.39 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:06:28.42 SS.reconfigure SSinhale.with maxSpeed: 80 @14:06:28.57 SS.seek 25ml @14:06:32.29 Vacuum too high -- waiting up to 40s @14:06:33.53 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:06:33.57 SS.reconfigure SSinhale.with maxSpeed: 72 @14:06:33.67 SS.seek 25ml @14:06:37.82 Vacuum too high -- waiting up to 40s @14:06:38.90 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:06:38.92 SS.reconfigure SSinhale.with maxSpeed: 65 @14:06:38.96 SS.seek 25ml @14:06:43.38 Vacuum too high -- waiting up to 40s @14:06:44.21 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:06:44.23 SS.reconfigure SSinhale.with maxSpeed: 59 @14:06:44.28 SS.seek 25ml @14:06:48.64 Stroke completed -- waiting for pressure to equalize ... @14:06:51.21 Intake.select :close @14:06:51.91 SSV.dial :exhaust @14:06:52.68 Exhaust.select :open @14:06:52.79 SS.reconfigure SSexhale @14:06:52.84 SS.seek 0ml @14:07:01.75 Exhaust.select :close @14:07:01.87 SSV.dial CBV @14:07:01.90
CBV.dial SSV @14:07:02.68 Allowing 130s for 25.0ml of remaining 1475.0ml @14:07:02.69 Intake.select :open @14:07:04.93 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:07:04.96 SS.reconfigure SSinhale.with maxSpeed: 59 @14:07:05.06 SS.seek 25ml @14:07:11.77 ATV.dial :waste @14:07:11.80 ARV.dial between carrier and air1 @14:07:11.89
Vacuum too high -- waiting up to 40s @14:07:11.92 ASV.dial :waste1 @14:07:12.71
Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:07:12.73 SS.reconfigure SSinhale.with maxSpeed: 53 @14:07:12.78 SS.seek 25ml @14:07:39.69 Stroke completed -- waiting for pressure to equalize ... @14:07:42.27 Intake.select :close @14:07:42.42 SSV.dial :exhaust @14:07:43.17 Exhaust.select :open @14:07:43.29 SS.reconfigure SSexhale @14:07:43.34 SS.seek 0ml @14:07:51.99 Exhaust.select :close @14:07:52.11 SSV.dial CBV @14:07:52.14
CBV.dial SSV @14:07:52.95 Allowing 130s for 25.0ml of remaining 1450.0ml @14:07:52.97 Intake.select :open @14:07:55.25 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:07:55.29 SS.reconfigure SSinhale.with maxSpeed: 53 @14:07:55.39 SS.seek 25ml @14:08:29.49 Stroke completed -- waiting for pressure to equalize ... @14:08:32.05 Intake.select :close @14:08:32.21 SSV.dial :exhaust @14:08:32.96 Exhaust.select :open @14:08:33.08 SS.reconfigure SSexhale @14:08:33.13 SS.seek 0ml @14:08:42.06 Exhaust.select :close @14:08:42.18 SSV.dial CBV @14:08:42.21
CBV.dial SSV @14:08:43.00 Allowing 130s for 25.0ml of remaining 1425.0ml @14:08:43.01 Intake.select :open @14:08:45.19 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:08:45.20 SS.reconfigure SSinhale.with maxSpeed: 53 @14:08:45.25 SS.seek 25ml @14:09:05.29 Vacuum too high -- waiting up to 40s @14:09:06.11 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:09:06.13 SS.reconfigure SSinhale.with maxSpeed: 48 @14:09:06.18 SS.seek 25ml @14:09:21.61 Stroke completed -- waiting for pressure to equalize ... @14:09:24.19 Intake.select :close @14:09:24.34 SSV.dial :exhaust @14:09:25.10 Exhaust.select :open @14:09:25.22 SS.reconfigure SSexhale @14:09:25.27 SS.seek 0ml @14:09:34.30 Exhaust.select :close @14:09:34.47 SSV.dial CBV @14:09:34.53
CBV.dial SSV @14:09:35.34 Allowing 130s for 25.0ml of remaining 1400.0ml @14:09:35.35 Intake.select :open @14:09:38.08 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:09:38.10 SS.reconfigure SSinhale.with maxSpeed: 48 @14:09:38.15 SS.seek 25ml @14:10:15.43 Stroke completed -- waiting for pressure to equalize ... @14:10:17.62 Intake.select :close @14:10:17.78 SSV.dial :exhaust @14:10:18.56 Exhaust.select :open @14:10:18.67 SS.reconfigure SSexhale @14:10:18.73 SS.seek 0ml @14:10:27.50 Exhaust.select :close @14:10:27.62 SSV.dial CBV @14:10:27.65
CBV.dial SSV @14:10:28.43 Allowing 130s for 25.0ml of remaining 1375.0ml @14:10:28.44 Intake.select :open @14:10:30.61 Intake @34.4psia, vacuum from 20.0 to 24.0psi @14:10:30.63 SS.reconfigure SSinhale.with maxSpeed: 48 @14:10:30.68 SS.seek 25ml @14:11:04.13 GoodNews.email "At 14:00:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tundeployv2.rb:115\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "Started 18tundeployv2 phase mbaPhase" @14:11:44.25
Stroke completed -- waiting for pressure to equalize ... @14:11:44.28 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:11:44.30 Retry #2 of 10 after 10:00 @14:11:44.96
Intake.select :close @14:11:45.11 SSV.dial :exhaust @14:11:45.86 Exhaust.select :open @14:11:45.98 SS.reconfigure SSexhale @14:11:46.03 SS.seek 0ml @14:11:54.97 Exhaust.select :close @14:11:55.09 SSV.dial CBV @14:11:55.12
CBV.dial SSV @14:11:55.90 Allowing 130s for 25.0ml of remaining 1350.0ml @14:11:55.91 Intake.select :open @14:11:58.08 Intake @34.4psia, vacuum from 20.0 to 24.0psi @14:11:58.10 SS.reconfigure SSinhale.with maxSpeed: 48 @14:11:58.15 SS.seek 25ml @14:12:35.38 Stroke completed -- waiting for pressure to equalize ... @14:12:37.96 Intake.select :close @14:12:38.12 SSV.dial :exhaust @14:12:38.86 Exhaust.select :open @14:12:38.98 SS.reconfigure SSexhale @14:12:39.03 SS.seek 0ml @14:12:47.92 Exhaust.select :close @14:12:48.04 SSV.dial CBV @14:12:48.07
CBV.dial SSV @14:12:48.86 Allowing 130s for 25.0ml of remaining 1325.0ml @14:12:48.87 Intake.select :open @14:12:51.04 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:12:51.06 SS.reconfigure SSinhale.with maxSpeed: 48 @14:12:51.10 SS.seek 25ml @14:13:28.55 Stroke completed -- waiting for pressure to equalize ... @14:13:31.11 Intake.select :close @14:13:31.27 SSV.dial :exhaust @14:13:32.02 Exhaust.select :open @14:13:32.14 SS.reconfigure SSexhale @14:13:32.19 SS.seek 0ml @14:13:40.89 Exhaust.select :close @14:13:41.01 SSV.dial CBV @14:13:41.04
CBV.dial SSV @14:13:41.82 Allowing 130s for 25.0ml of remaining 1300.0ml @14:13:41.83 Intake.select :open @14:13:44.00 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:13:44.02 SS.reconfigure SSinhale.with maxSpeed: 48 @14:13:44.07 SS.seek 25ml @14:14:21.52 Stroke completed -- waiting for pressure to equalize ... @14:14:24.11 Intake.select :close @14:14:24.26 SSV.dial :exhaust @14:14:25.03 Exhaust.select :open @14:14:25.59 SS.reconfigure SSexhale @14:14:25.72 SS.seek 0ml @14:14:34.44 Exhaust.select :close @14:14:34.56 SSV.dial CBV @14:14:34.59
CBV.dial SSV @14:14:35.39 Allowing 130s for 25.0ml of remaining 1275.0ml @14:14:35.40 Intake.select :open @14:14:37.58 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:14:37.59 SS.reconfigure SSinhale.with maxSpeed: 48 @14:14:37.64 SS.seek 25ml @14:15:15.17 Stroke completed -- waiting for pressure to equalize ... @14:15:17.77 Intake.select :close @14:15:17.93 SSV.dial :exhaust @14:15:18.67 Exhaust.select :open @14:15:18.79 SS.reconfigure SSexhale @14:15:18.84 SS.seek 0ml @14:15:28.08 Exhaust.select :close @14:15:28.20 SSV.dial CBV @14:15:28.23
CBV.dial SSV @14:15:29.01 Allowing 130s for 25.0ml of remaining 1250.0ml @14:15:29.02 Intake.select :open @14:15:31.19 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:15:31.21 SS.reconfigure SSinhale.with maxSpeed: 48 @14:15:31.26 SS.seek 25ml @14:16:08.53 Stroke completed -- waiting for pressure to equalize ... @14:16:11.17 Intake.select :close @14:16:11.32 SSV.dial :exhaust @14:16:12.08 Exhaust.select :open @14:16:12.20 SS.reconfigure SSexhale @14:16:12.25 SS.seek 0ml @14:16:21.13 Exhaust.select :close @14:16:21.25 SSV.dial CBV @14:16:21.28
CBV.dial SSV @14:16:22.07 Allowing 130s for 25.0ml of remaining 1225.0ml @14:16:22.08 Intake.select :open @14:16:24.25 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:16:24.27 SS.reconfigure SSinhale.with maxSpeed: 48 @14:16:24.32 SS.seek 25ml @14:17:01.83 Stroke completed -- waiting for pressure to equalize ... @14:17:04.39 Intake.select :close @14:17:04.55 SSV.dial :exhaust @14:17:05.30 Exhaust.select :open @14:17:05.42 SS.reconfigure SSexhale @14:17:05.47 SS.seek 0ml @14:17:14.28 Exhaust.select :close @14:17:14.40 SSV.dial CBV @14:17:14.43
CBV.dial SSV @14:17:15.22 Allowing 130s for 25.0ml of remaining 1200.0ml @14:17:15.23 Intake.select :open @14:17:17.40 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:17:17.42 SS.reconfigure SSinhale.with maxSpeed: 48 @14:17:17.47 SS.seek 25ml @14:17:40.04 Vacuum too high -- waiting up to 40s @14:17:40.87 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:17:40.89 SS.reconfigure SSinhale.with maxSpeed: 43 @14:17:40.94 SS.seek 25ml @14:17:57.38 Stroke completed -- waiting for pressure to equalize ... @14:17:59.96 Intake.select :close @14:18:00.11 SSV.dial :exhaust @14:18:00.86 Exhaust.select :open @14:18:00.98 SS.reconfigure SSexhale @14:18:01.56 SS.seek 0ml @14:18:10.33 Exhaust.select :close @14:18:10.45 SSV.dial CBV @14:18:10.48
CBV.dial SSV @14:18:11.28 Allowing 130s for 25.0ml of remaining 1175.0ml @14:18:11.29 Intake.select :open @14:18:13.46 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:18:13.48 SS.reconfigure SSinhale.with maxSpeed: 43 @14:18:13.53 SS.seek 25ml @14:18:55.17 Stroke completed -- waiting for pressure to equalize ... @14:18:57.77 Intake.select :close @14:18:57.92 SSV.dial :exhaust @14:18:58.67 Exhaust.select :open @14:18:58.79 SS.reconfigure SSexhale @14:18:58.84 SS.seek 0ml @14:19:07.64 Exhaust.select :close @14:19:07.76 SSV.dial CBV @14:19:07.79
CBV.dial SSV @14:19:08.57 Allowing 130s for 25.0ml of remaining 1150.0ml @14:19:08.58 Intake.select :open @14:19:10.75 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:19:10.77 SS.reconfigure SSinhale.with maxSpeed: 43 @14:19:10.83 SS.seek 25ml @14:19:52.49 Stroke completed -- waiting for pressure to equalize ... @14:19:55.07 Intake.select :close @14:19:55.23 SSV.dial :exhaust @14:19:55.99 Exhaust.select :open @14:19:56.11 SS.reconfigure SSexhale @14:19:56.16 SS.seek 0ml @14:20:04.95 Exhaust.select :close @14:20:05.07 SSV.dial CBV @14:20:05.10
CBV.dial SSV @14:20:05.89 Allowing 130s for 25.0ml of remaining 1125.0ml @14:20:05.90 Intake.select :open @14:20:08.07 Intake @34.5psia, vacuum from 20.0 to 24.0psi @14:20:08.09 SS.reconfigure SSinhale.with maxSpeed: 43 @14:20:08.14 SS.seek 25ml @14:20:49.77 Stroke completed -- waiting for pressure to equalize ... @14:20:52.34 Intake.select :close @14:20:52.49 SSV.dial :exhaust @14:20:53.25 Exhaust.select :open @14:20:53.37 SS.reconfigure SSexhale @14:20:53.42 SS.seek 0ml @14:21:02.19 Exhaust.select :close @14:21:02.31 SSV.dial CBV @14:21:02.34
CBV.dial SSV @14:21:03.15 Allowing 130s for 25.0ml of remaining 1100.0ml @14:21:03.16 Intake.select :open @14:21:05.33 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:21:05.35 SS.reconfigure SSinhale.with maxSpeed: 43 @14:21:05.39 SS.seek 25ml @14:21:44.33 GoodNews.email "At 14:00:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tundeployv2.rb:115\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "Started 18tundeployv2 phase mbaPhase" @14:22:24.45
Stroke completed -- waiting for pressure to equalize ... @14:22:24.48 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:22:24.50 Retry #3 of 10 after 10:00 @14:22:25.16
Intake.select :close @14:22:25.31 SSV.dial :exhaust @14:22:26.07 Exhaust.select :open @14:22:26.18 SS.reconfigure SSexhale @14:22:26.23 SS.seek 0ml @14:22:34.92 Exhaust.select :close @14:22:35.04 SSV.dial CBV @14:22:35.07
CBV.dial SSV @14:22:35.86 Allowing 130s for 25.0ml of remaining 1075.0ml @14:22:35.87 Intake.select :open @14:22:38.04 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:22:38.06 SS.reconfigure SSinhale.with maxSpeed: 43 @14:22:38.11 SS.seek 25ml @14:23:19.74 Stroke completed -- waiting for pressure to equalize ... @14:23:22.83 Intake.select :close @14:23:22.99 SSV.dial :exhaust @14:23:23.74 Exhaust.select :open @14:23:23.86 SS.reconfigure SSexhale @14:23:23.91 SS.seek 0ml @14:23:32.61 Exhaust.select :close @14:23:32.73 SSV.dial CBV @14:23:32.76
CBV.dial SSV @14:23:33.53 Allowing 130s for 25.0ml of remaining 1050.0ml @14:23:33.54 Intake.select :open @14:23:35.71 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:23:35.73 SS.reconfigure SSinhale.with maxSpeed: 43 @14:23:35.78 SS.seek 25ml @14:24:17.44 Stroke completed -- waiting for pressure to equalize ... @14:24:20.66 Intake.select :close @14:24:20.82 SSV.dial :exhaust @14:24:21.58 Exhaust.select :open @14:24:21.70 SS.reconfigure SSexhale @14:24:21.75 SS.seek 0ml @14:24:30.58 Exhaust.select :close @14:24:30.70 SSV.dial CBV @14:24:30.73
CBV.dial SSV @14:24:31.50 Allowing 130s for 25.0ml of remaining 1025.0ml @14:24:31.51 Intake.select :open @14:24:33.68 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:24:33.70 SS.reconfigure SSinhale.with maxSpeed: 43 @14:24:33.75 SS.seek 25ml @14:25:01.91 Vacuum too high -- waiting up to 40s @14:25:02.73 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:25:02.75 SS.reconfigure SSinhale.with maxSpeed: 39 @14:25:02.79 SS.seek 25ml @14:25:17.63 Stroke completed -- waiting for pressure to equalize ... @14:25:20.21 Intake.select :close @14:25:20.37 SSV.dial :exhaust @14:25:21.13 Exhaust.select :open @14:25:21.25 SS.reconfigure SSexhale @14:25:21.30 SS.seek 0ml @14:25:30.16 Exhaust.select :close @14:25:30.28 SSV.dial CBV @14:25:30.31
CBV.dial SSV @14:25:31.09 Allowing 130s for 25.0ml of remaining 1000.0ml @14:25:31.10 Intake.select :open @14:25:33.27 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:25:33.29 SS.reconfigure SSinhale.with maxSpeed: 39 @14:25:33.34 SS.seek 25ml @14:26:19.10 Stroke completed -- waiting for pressure to equalize ... @14:26:22.30 Intake.select :close @14:26:22.45 SSV.dial :exhaust @14:26:23.22 Exhaust.select :open @14:26:23.34 SS.reconfigure SSexhale @14:26:23.39 SS.seek 0ml @14:26:32.52 Exhaust.select :close @14:26:32.64 SSV.dial CBV @14:26:32.67
CBV.dial SSV @14:26:33.45 Allowing 130s for 25.0ml of remaining 975.0ml @14:26:33.46 Intake.select :open @14:26:35.63 Intake @34.5psia, vacuum from 20.0 to 24.0psi @14:26:35.65 SS.reconfigure SSinhale.with maxSpeed: 39 @14:26:35.70 SS.seek 25ml @14:27:21.13 Stroke completed -- waiting for pressure to equalize ... @14:27:24.25 Intake.select :close @14:27:24.40 SSV.dial :exhaust @14:27:25.17 Exhaust.select :open @14:27:25.29 SS.reconfigure SSexhale @14:27:25.34 SS.seek 0ml @14:27:34.10 Exhaust.select :close @14:27:34.22 SSV.dial CBV @14:27:34.25
CBV.dial SSV @14:27:35.03 Allowing 130s for 25.0ml of remaining 950.0ml @14:27:35.04 Intake.select :open @14:27:37.21 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:27:37.23 SS.reconfigure SSinhale.with maxSpeed: 39 @14:27:37.28 SS.seek 25ml @14:28:22.99 Stroke completed -- waiting for pressure to equalize ... @14:28:26.20 Intake.select :close @14:28:26.36 SSV.dial :exhaust @14:28:27.14 Exhaust.select :open @14:28:27.25 SS.reconfigure SSexhale @14:28:27.30 SS.seek 0ml @14:28:36.05 Exhaust.select :close @14:28:36.17 SSV.dial CBV @14:28:36.20
CBV.dial SSV @14:28:36.96 Allowing 130s for 25.0ml of remaining 925.0ml @14:28:36.97 Intake.select :open @14:28:39.14 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:28:39.16 SS.reconfigure SSinhale.with maxSpeed: 39 @14:28:39.21 SS.seek 25ml @14:29:23.41 Vacuum too high -- waiting up to 40s @14:29:24.23 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:29:24.25 SS.reconfigure SSinhale.with maxSpeed: 35 @14:29:24.30 SS.seek 25ml @14:29:26.14 Stroke completed -- waiting for pressure to equalize ... @14:29:28.73 Intake.select :close @14:29:28.88 SSV.dial :exhaust @14:29:29.66 Exhaust.select :open @14:29:29.78 SS.reconfigure SSexhale @14:29:29.83 SS.seek 0ml @14:29:39.11 Exhaust.select :close @14:29:39.23 SSV.dial CBV @14:29:39.26
CBV.dial SSV @14:29:40.03 Allowing 130s for 25.0ml of remaining 900.0ml @14:29:40.04 Intake.select :open @14:29:42.21 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:29:42.23 SS.reconfigure SSinhale.with maxSpeed: 35 @14:29:42.27 SS.seek 25ml @14:30:32.72 Stroke completed -- waiting for pressure to equalize ... @14:30:35.90 Intake.select :close @14:30:36.05 SSV.dial :exhaust @14:30:36.83 Exhaust.select :open @14:30:36.95 SS.reconfigure SSexhale @14:30:37.00 SS.seek 0ml @14:30:45.99 Exhaust.select :close @14:30:46.11 SSV.dial CBV @14:30:46.14
CBV.dial SSV @14:30:46.92 Allowing 130s for 25.0ml of remaining 875.0ml @14:30:46.93 Intake.select :open @14:30:49.10 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:30:49.63 SS.reconfigure SSinhale.with maxSpeed: 35 @14:30:49.71 SS.seek 25ml @14:31:40.14 Stroke completed -- waiting for pressure to equalize ... @14:31:43.36 Intake.select :close @14:31:43.52 SSV.dial :exhaust @14:31:44.29 Exhaust.select :open @14:31:44.40 SS.reconfigure SSexhale @14:31:44.45 SS.seek 0ml @14:31:53.52 Exhaust.select :close @14:31:53.64 SSV.dial CBV @14:31:53.67
CBV.dial SSV @14:31:54.45 Allowing 130s for 25.0ml of remaining 850.0ml @14:31:54.46 Intake.select :open @14:31:56.63 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:31:56.65 SS.reconfigure SSinhale.with maxSpeed: 35 @14:31:56.70 SS.seek 25ml @14:32:24.53 GoodNews.email "At 14:00:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tundeployv2.rb:115\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "Started 18tundeployv2 phase mbaPhase" @14:33:04.64 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:33:04.67
Stroke completed -- waiting for pressure to equalize ... @14:33:04.70 Retry #4 of 10 after 10:00 @14:33:05.39
Intake.select :close @14:33:05.55 SSV.dial :exhaust @14:33:06.32 Exhaust.select :open @14:33:06.43 SS.reconfigure SSexhale @14:33:06.48 SS.seek 0ml @14:33:15.20 Exhaust.select :close @14:33:15.32 SSV.dial CBV @14:33:15.35
CBV.dial SSV @14:33:16.12 Allowing 130s for 25.0ml of remaining 825.0ml @14:33:16.13 Intake.select :open @14:33:18.30 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:33:18.32 SS.reconfigure SSinhale.with maxSpeed: 35 @14:33:18.37 SS.seek 25ml @14:34:09.09 Stroke completed -- waiting for pressure to equalize ... @14:34:12.91 Intake.select :close @14:34:13.06 SSV.dial :exhaust @14:34:13.83 Exhaust.select :open @14:34:13.95 SS.reconfigure SSexhale @14:34:14.00 SS.seek 0ml @14:34:22.74 Exhaust.select :close @14:34:22.86 SSV.dial CBV @14:34:22.89
CBV.dial SSV @14:34:23.67 Allowing 130s for 25.0ml of remaining 800.0ml @14:34:23.68 Intake.select :open @14:34:25.85 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:34:25.87 SS.reconfigure SSinhale.with maxSpeed: 35 @14:34:25.92 SS.seek 25ml @14:35:16.66 Stroke completed -- waiting for pressure to equalize ... @14:35:20.47 Intake.select :close @14:35:20.63 SSV.dial :exhaust @14:35:21.39 Exhaust.select :open @14:35:21.51 SS.reconfigure SSexhale @14:35:21.56 SS.seek 0ml @14:35:30.66 Exhaust.select :close @14:35:30.78 SSV.dial CBV @14:35:30.81
CBV.dial SSV @14:35:31.59 Allowing 130s for 25.0ml of remaining 775.0ml @14:35:31.60 Intake.select :open @14:35:33.78 Intake @34.6psia, vacuum from 20.0 to 24.0psi @14:35:33.80 SS.reconfigure SSinhale.with maxSpeed: 35 @14:35:33.85 SS.seek 25ml @14:36:01.38 Vacuum too high -- waiting up to 40s @14:36:02.49 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:36:02.51 SS.reconfigure SSinhale.with maxSpeed: 32 @14:36:02.56 SS.seek 25ml @14:36:27.34 Stroke completed -- waiting for pressure to equalize ... @14:36:30.57 Intake.select :close @14:36:30.73 SSV.dial :exhaust @14:36:31.50 Exhaust.select :open @14:36:31.62 SS.reconfigure SSexhale @14:36:31.67 SS.seek 0ml @14:36:40.73 Exhaust.select :close @14:36:40.85 SSV.dial CBV @14:36:40.88
CBV.dial SSV @14:36:41.65 Allowing 130s for 25.0ml of remaining 750.0ml @14:36:41.66 Intake.select :open @14:36:43.83 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:36:43.85 SS.reconfigure SSinhale.with maxSpeed: 32 @14:36:43.90 SS.seek 25ml @14:37:38.81 Stroke completed -- waiting for pressure to equalize ... @14:37:42.63 Intake.select :close @14:37:42.78 SSV.dial :exhaust @14:37:43.56 Exhaust.select :open @14:37:43.67 SS.reconfigure SSexhale @14:37:43.72 SS.seek 0ml @14:37:52.77 Exhaust.select :close @14:37:52.89 SSV.dial CBV @14:37:52.92
CBV.dial SSV @14:37:53.70 Allowing 130s for 25.0ml of remaining 725.0ml @14:37:53.71 Intake.select :open @14:37:55.88 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:37:55.90 SS.reconfigure SSinhale.with maxSpeed: 32 @14:37:55.95 SS.seek 25ml @14:38:50.99 Stroke completed -- waiting for pressure to equalize ... @14:38:54.81 Intake.select :close @14:38:54.96 SSV.dial :exhaust @14:38:55.72 Exhaust.select :open @14:38:55.84 SS.reconfigure SSexhale @14:38:55.89 SS.seek 0ml @14:39:04.97 Exhaust.select :close @14:39:05.09 SSV.dial CBV @14:39:05.12
CBV.dial SSV @14:39:05.89 Allowing 130s for 25.0ml of remaining 700.0ml @14:39:05.90 Intake.select :open @14:39:08.07 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:39:08.09 SS.reconfigure SSinhale.with maxSpeed: 32 @14:39:08.14 SS.seek 25ml @14:40:03.17 Stroke completed -- waiting for pressure to equalize ... @14:40:07.59 Intake.select :close @14:40:07.73 SSV.dial :exhaust @14:40:08.52 Exhaust.select :open @14:40:08.63 SS.reconfigure SSexhale @14:40:08.68 SS.seek 0ml @14:40:18.03 Exhaust.select :close @14:40:18.15 SSV.dial CBV @14:40:18.18
CBV.dial SSV @14:40:18.95 Allowing 130s for 25.0ml of remaining 675.0ml @14:40:18.96 Intake.select :open @14:40:21.14 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:40:21.15 SS.reconfigure SSinhale.with maxSpeed: 32 @14:40:21.20 SS.seek 25ml @14:40:59.73 Vacuum too high -- waiting up to 40s @14:41:00.82 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:41:00.84 SS.reconfigure SSinhale.with maxSpeed: 29 @14:41:00.89 SS.seek 25ml @14:41:19.53 Stroke completed -- waiting for pressure to equalize ... @14:41:23.38 Intake.select :close @14:41:23.54 SSV.dial :exhaust @14:41:24.31 Exhaust.select :open @14:41:24.42 SS.reconfigure SSexhale @14:41:24.47 SS.seek 0ml @14:41:33.45 Exhaust.select :close @14:41:33.57 SSV.dial CBV @14:41:33.60
CBV.dial SSV @14:41:34.37 Allowing 130s for 25.0ml of remaining 650.0ml @14:41:34.38 Intake.select :open @14:41:36.55 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:41:36.57 SS.reconfigure SSinhale.with maxSpeed: 29 @14:41:36.62 SS.seek 25ml @14:42:37.19 Stroke completed -- waiting for pressure to equalize ... @14:42:41.64 Intake.select :close @14:42:41.79 SSV.dial :exhaust @14:42:42.58 Exhaust.select :open @14:42:42.69 SS.reconfigure SSexhale @14:42:42.74 SS.seek 0ml @14:42:51.59 Exhaust.select :close @14:42:51.71 SSV.dial CBV @14:42:51.74
CBV.dial SSV @14:42:52.51 Allowing 130s for 25.0ml of remaining 625.0ml @14:42:52.52 Intake.select :open @14:42:54.69 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:42:54.71 SS.reconfigure SSinhale.with maxSpeed: 29 @14:42:54.76 SS.seek 25ml @14:43:04.73 GoodNews.email "At 14:00:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tundeployv2.rb:115\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "Started 18tundeployv2 phase mbaPhase" @14:43:44.84 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:43:44.85 Retry #5 of 10 after 10:00 @14:43:55.38
Stroke completed -- waiting for pressure to equalize ... @14:43:59.82 Intake.select :close @14:43:59.97 SSV.dial :exhaust @14:44:00.76 Exhaust.select :open @14:44:00.87 SS.reconfigure SSexhale @14:44:00.92 SS.seek 0ml @14:44:09.94 Exhaust.select :close @14:44:10.06 SSV.dial CBV @14:44:10.09
CBV.dial SSV @14:44:10.87 Allowing 130s for 25.0ml of remaining 600.0ml @14:44:10.88 Intake.select :open @14:44:13.59 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:44:13.60 SS.reconfigure SSinhale.with maxSpeed: 29 @14:44:13.65 SS.seek 25ml @14:45:14.27 Stroke completed -- waiting for pressure to equalize ... @14:45:18.79 Intake.select :close @14:45:18.95 SSV.dial :exhaust @14:45:19.74 Exhaust.select :open @14:45:19.85 SS.reconfigure SSexhale @14:45:19.90 SS.seek 0ml @14:45:29.05 Exhaust.select :close @14:45:29.17 SSV.dial CBV @14:45:29.20
CBV.dial SSV @14:45:29.98 Allowing 130s for 25.0ml of remaining 575.0ml @14:45:29.99 Intake.select :open @14:45:32.16 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:45:32.18 SS.reconfigure SSinhale.with maxSpeed: 29 @14:45:32.23 SS.seek 25ml @14:46:09.01 Vacuum too high -- waiting up to 40s @14:46:10.10 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:46:10.12 SS.reconfigure SSinhale.with maxSpeed: 26 @14:46:10.17 SS.seek 25ml @14:46:36.77 Stroke completed -- waiting for pressure to equalize ... @14:46:41.21 Intake.select :close @14:46:41.37 SSV.dial :exhaust @14:46:42.13 Exhaust.select :open @14:46:42.25 SS.reconfigure SSexhale @14:46:42.30 SS.seek 0ml @14:46:51.44 Exhaust.select :close @14:46:51.56 SSV.dial CBV @14:46:51.59
CBV.dial SSV @14:46:52.37 Allowing 130s for 25.0ml of remaining 550.0ml @14:46:52.38 Intake.select :open @14:46:54.55 Intake @34.6psia, vacuum from 20.0 to 24.0psi @14:46:54.57 SS.reconfigure SSinhale.with maxSpeed: 26 @14:46:54.62 SS.seek 25ml @14:48:02.02 Stroke completed -- waiting for pressure to equalize ... @14:48:06.44 Intake.select :close @14:48:06.59 SSV.dial :exhaust @14:48:07.38 Exhaust.select :open @14:48:07.49 SS.reconfigure SSexhale @14:48:07.54 SS.seek 0ml @14:48:16.66 Exhaust.select :close @14:48:16.78 SSV.dial CBV @14:48:16.81
CBV.dial SSV @14:48:17.59 Allowing 130s for 25.0ml of remaining 525.0ml @14:48:17.60 Intake.select :open @14:48:19.77 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:48:19.79 SS.reconfigure SSinhale.with maxSpeed: 26 @14:48:19.84 SS.seek 25ml @14:49:27.06 Stroke completed -- waiting for pressure to equalize ... @14:49:32.08 Intake.select :close @14:49:32.24 SSV.dial :exhaust @14:49:32.98 Exhaust.select :open @14:49:33.10 SS.reconfigure SSexhale @14:49:33.15 SS.seek 0ml @14:49:42.24 Exhaust.select :close @14:49:42.36 SSV.dial CBV @14:49:42.39
CBV.dial SSV @14:49:43.17 Allowing 130s for 25.0ml of remaining 500.0ml @14:49:43.18 Intake.select :open @14:49:45.35 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:49:45.37 SS.reconfigure SSinhale.with maxSpeed: 26 @14:49:45.42 SS.seek 25ml @14:50:36.91 Vacuum too high -- waiting up to 40s @14:50:38.00 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:50:38.02 SS.reconfigure SSinhale.with maxSpeed: 23 @14:50:38.07 SS.seek 25ml @14:50:56.22 Stroke completed -- waiting for pressure to equalize ... @14:51:00.69 Intake.select :close @14:51:00.85 SSV.dial :exhaust @14:51:01.63 Exhaust.select :open @14:51:01.74 SS.reconfigure SSexhale @14:51:01.79 SS.seek 0ml @14:51:10.63 Exhaust.select :close @14:51:10.75 SSV.dial CBV @14:51:10.78
CBV.dial SSV @14:51:11.56 Allowing 130s for 25.0ml of remaining 475.0ml @14:51:11.57 Intake.select :open @14:51:13.74 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:51:13.76 SS.reconfigure SSinhale.with maxSpeed: 23 @14:51:13.81 SS.seek 25ml @14:52:30.17 Stroke completed -- waiting for pressure to equalize ... @14:52:34.63 Intake.select :close @14:52:34.78 SSV.dial :exhaust @14:52:35.57 Exhaust.select :open @14:52:35.68 SS.reconfigure SSexhale @14:52:35.73 SS.seek 0ml @14:52:44.83 Exhaust.select :close @14:52:44.95 SSV.dial CBV @14:52:44.98
CBV.dial SSV @14:52:45.76 Allowing 130s for 25.0ml of remaining 450.0ml @14:52:45.77 Intake.select :open @14:52:47.94 Intake @34.4psia, vacuum from 20.0 to 24.0psi @14:52:47.96 SS.reconfigure SSinhale.with maxSpeed: 23 @14:52:48.01 SS.seek 25ml @14:53:44.88 GoodNews.email "At 14:00:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tundeployv2.rb:115\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "Started 18tundeployv2 phase mbaPhase" @14:54:24.99 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:54:25.02
Stroke completed -- waiting for pressure to equalize ... @14:54:25.07 Retry #6 of 10 after 10:00 @14:54:25.75
Intake.select :close @14:54:25.91 SSV.dial :exhaust @14:54:26.66 Exhaust.select :open @14:54:27.29 SS.reconfigure SSexhale @14:54:27.37 SS.seek 0ml @14:54:36.47 Exhaust.select :close @14:54:36.59 SSV.dial CBV @14:54:36.64
CBV.dial SSV @14:54:37.39 Allowing 130s for 25.0ml of remaining 425.0ml @14:54:37.40 Intake.select :open @14:54:39.57 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:54:39.59 SS.reconfigure SSinhale.with maxSpeed: 23 @14:54:39.64 SS.seek 25ml @14:55:55.72 Stroke completed -- waiting for pressure to equalize ... @14:56:01.48 Intake.select :close @14:56:01.64 SSV.dial :exhaust @14:56:02.40 Exhaust.select :open @14:56:02.51 SS.reconfigure SSexhale @14:56:02.56 SS.seek 0ml @14:56:11.38 Exhaust.select :close @14:56:11.50 SSV.dial CBV @14:56:11.53
CBV.dial SSV @14:56:12.32 Allowing 130s for 25.0ml of remaining 400.0ml @14:56:12.33 Intake.select :open @14:56:14.50 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:56:14.52 SS.reconfigure SSinhale.with maxSpeed: 23 @14:56:14.56 SS.seek 25ml @14:57:31.02 Stroke completed -- waiting for pressure to equalize ... @14:57:36.69 Intake.select :close @14:57:36.84 SSV.dial :exhaust @14:57:37.60 Exhaust.select :open @14:57:37.71 SS.reconfigure SSexhale @14:57:37.76 SS.seek 0ml @14:57:46.56 Exhaust.select :close @14:57:46.68 SSV.dial CBV @14:57:46.71
CBV.dial SSV @14:57:47.48 Allowing 130s for 25.0ml of remaining 375.0ml @14:57:47.49 Intake.select :open @14:57:49.66 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:57:49.68 SS.reconfigure SSinhale.with maxSpeed: 23 @14:57:49.73 SS.seek 25ml @14:58:47.79 Vacuum too high -- waiting up to 40s @14:58:49.12 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:58:49.14 SS.reconfigure SSinhale.with maxSpeed: 21 @14:58:49.19 SS.seek 25ml @14:59:09.48 Stroke completed -- waiting for pressure to equalize ... @14:59:15.73 Intake.select :close @14:59:15.89 SSV.dial :exhaust @14:59:16.66 Exhaust.select :open @14:59:16.78 SS.reconfigure SSexhale @14:59:16.83 SS.seek 0ml @14:59:25.95 Exhaust.select :close @14:59:26.07 SSV.dial CBV @14:59:26.10
CBV.dial SSV @14:59:26.87 Allowing 130s for 25.0ml of remaining 350.0ml @14:59:26.88 Intake.select :open @14:59:29.05 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:59:29.07 SS.reconfigure SSinhale.with maxSpeed: 21 @14:59:29.13 SS.seek 25ml @15:00:52.41 Stroke completed -- waiting for pressure to equalize ... @15:00:58.79 Intake.select :close @15:00:58.95 SSV.dial :exhaust @15:00:59.72 Exhaust.select :open @15:00:59.84 SS.reconfigure SSexhale @15:00:59.89 SS.seek 0ml @15:01:08.81 Exhaust.select :close @15:01:08.93 SSV.dial CBV @15:01:08.96
CBV.dial SSV @15:01:09.73 Allowing 130s for 25.0ml of remaining 325.0ml @15:01:09.74 Intake.select :open @15:01:11.91 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:01:11.93 SS.reconfigure SSinhale.with maxSpeed: 21 @15:01:11.98 SS.seek 25ml @15:02:35.66 Stroke completed -- waiting for pressure to equalize ... @15:02:42.57 Intake.select :close @15:02:42.71 SSV.dial :exhaust @15:02:43.48 Exhaust.select :open @15:02:43.60 SS.reconfigure SSexhale @15:02:43.65 SS.seek 0ml @15:02:52.89 Exhaust.select :close @15:02:53.01 SSV.dial CBV @15:02:53.04
CBV.dial SSV @15:02:53.81 Allowing 130s for 25.0ml of remaining 300.0ml @15:02:53.82 Intake.select :open @15:02:55.99 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:02:56.01 SS.reconfigure SSinhale.with maxSpeed: 21 @15:02:56.06 SS.seek 25ml @15:03:39.29 Vacuum too high -- waiting up to 40s @15:03:40.61 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:03:40.63 SS.reconfigure SSinhale.with maxSpeed: 19 @15:03:40.68 SS.seek 25ml @15:04:25.11 GoodNews.email "At 14:00:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tundeployv2.rb:115\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "Started 18tundeployv2 phase mbaPhase" @15:05:05.23
Stroke completed -- waiting for pressure to equalize ... @15:05:05.26 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:05:05.28 Retry #7 of 10 after 10:00 @15:05:05.31
SS.reconfigure SSconfig @15:05:05.91 SS.hold @15:05:06.03 Intake.select :close @15:05:06.19 ShallowSampler::Clogged in MAIN -- progress too slow after 1225.0ml @15:05:06.20 SSV.dial :exhaust @15:05:06.98 Exhaust.select :open @15:05:07.09 SS.reconfigure SSexhale @15:05:07.14 SS.seek 0ml @15:05:16.02 Exhaust.select :close @15:05:16.14 Sampled 1225.0ml @15:05:16.16 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @15:05:16.17 Intake.select :close @15:05:16.23 CSV.dial :waste @15:05:16.31 CS.seek 0ml @15:05:16.40 CSV.dial :air @15:05:16.44 SSV.dial CBV,avoiding: :exhaust @15:05:16.48
CBV.dial SSV @15:05:17.23 CS.seek 10ml @15:05:18.37
CTV.dial :air @15:05:19.13 delay 15 seconds @15:05:34.16 SSV.dial :spit,avoiding: :exhaust @15:05:35.45 CTV.dial CSV,avoiding: :intake @15:05:35.50
SS.seek 0ml @15:05:35.63 SSV.dial CBV,avoiding: :exhaust @15:05:40.11 CBV.dial between SSV and waste2 @15:05:40.51 CTV.dial CSV,avoiding: :air @15:05:40.54
CSV.dial CTV @15:05:40.99 CS.reconfigure CSpressurize @15:05:41.04 CS.seek 0ml @15:06:06.51 BubblePoint: Intake @68.9psia, Outlet @13.1psia @15:06:06.52 CBV.dial SSV @15:06:06.87 SS.reconfigure SSconfig @15:06:06.92 SS.seek 10ml @15:06:12.31 delay 15 seconds @15:06:27.45 Evacuated: Intake @33.3psia, Outlet @-0.2psia @15:06:27.46 SSV.dial :spit,avoiding: :exhaust @15:06:28.78 SS.reconfigure SSexhale @15:06:28.83 SS.seek 0ml @15:06:33.97 SS.reconfigure SSfast @15:06:34.02 SS.seek 5ml @15:06:36.68 SSV.dial CBV,avoiding: :exhaust @15:06:36.71
CBV.dial SSV @15:06:38.01 CTV.dial :air,avoiding: :intake @15:06:39.30 CSV.dial CTV @15:06:39.35
SS.seek 10ml @15:06:40.34 CSV.dial :air @15:06:40.82 CS.reconfigure CSfast @15:06:40.87 CS.seek 0ml @15:06:42.10
SSV.dial :spit,avoiding: :exhaust @15:06:42.45 CS.coast @15:06:43.39
SS.reconfigure SSexhale @15:06:43.44 SS.seek 0ml @15:06:48.38 SS.reconfigure SSfast @15:06:48.43 SS.seek 5ml @15:06:51.10 SSV.dial CBV,avoiding: :exhaust @15:06:51.13
CBV.dial SSV @15:06:53.46 SSV.dial :spit,avoiding: :exhaust @15:06:54.74 SS.reconfigure SSconfig @15:06:54.79 SS.seek 0ml @15:06:57.94 SS.coast @15:06:57.99 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @15:06:58.00 Intake.select :close @15:06:58.08 CSV.dial :waste @15:06:58.22 SSV.dial CBV,avoiding: :exhaust @15:06:58.25
CBV.dial SSV @15:06:58.89 CS.seek 0ml @15:06:58.95 CSV.dial :air @15:06:59.70 CS.seek 10ml @15:07:00.12
CTV.dial :air @15:07:00.13 delay 15 seconds @15:07:15.16 SSV.dial :spit,avoiding: :exhaust @15:07:16.45 CTV.dial CSV,avoiding: :intake @15:07:16.49
SS.reconfigure SSexhale @15:07:16.60 SS.seek 0ml @15:07:16.67 SSV.dial CBV,avoiding: :exhaust @15:07:22.39 CBV.dial between SSV and waste2 @15:07:22.81 CSV.dial between air and CTV @15:07:23.13 CS.seek 8.328ml @15:07:27.81 CTV.dial CSV,avoiding: :air @15:07:27.84
CSV.dial CTV @15:07:28.15 CS.reconfigure CSpressurize @15:07:28.20 CS.seek 0ml @15:07:50.14 CSV.dial :air @15:07:50.62 CS.reconfigure CSfast @15:07:50.67 CS.seek 10ml @15:08:13.27 CSV.dial between air and CTV @15:08:13.61 CS.seek 3.15ml @15:08:29.37 CTV.dial CSV,avoiding: :air @15:08:29.40
CSV.dial CTV @15:08:29.71 CS.reconfigure CSpressurize @15:08:29.76 CS.seek 0ml @15:08:37.73 BubblePoint: Intake @68.0psia, Outlet @12.7psia @15:08:37.74 CBV.dial SSV @15:08:38.09 SS.reconfigure SSconfig @15:08:38.14 SS.seek 10ml @15:08:43.56 delay 15 seconds @15:08:58.69 Evacuated: Intake @37.6psia, Outlet @14.7psia @15:08:58.70 SSV.dial :spit,avoiding: :exhaust @15:09:00.01 SS.reconfigure SSexhale @15:09:00.07 SS.seek 0ml @15:09:05.22 SS.reconfigure SSfast @15:09:05.27 SS.seek 5ml @15:09:07.76 SSV.dial CBV,avoiding: :exhaust @15:09:07.79
CBV.dial SSV @15:09:09.10 CTV.dial :air,avoiding: :intake @15:09:10.40 CSV.dial CTV @15:09:10.44
SS.seek 10ml @15:09:11.43 CSV.dial :air @15:09:11.90 CS.reconfigure CSfast @15:09:11.95 CS.seek 0ml @15:09:12.85
SSV.dial :spit,avoiding: :exhaust @15:09:13.55 CS.coast @15:09:14.14
SS.reconfigure SSexhale @15:09:14.19 SS.seek 0ml @15:09:19.38 SS.reconfigure SSfast @15:09:19.43 SS.seek 5ml @15:09:21.88 SSV.dial CBV,avoiding: :exhaust @15:09:21.90
CBV.dial SSV @15:09:24.24 SSV.dial :spit,avoiding: :exhaust @15:09:25.53 SS.reconfigure SSconfig @15:09:25.59 SS.seek 0ml @15:09:28.75 SS.coast @15:09:28.80 Lysing: Adding 1.5ml lysis @15:09:29.27 CBV.dial between CSV and waste1,avoiding: :DBV @15:09:30.84 CTV.dial CSV @15:09:32.13 CSV.dial CSR @15:09:32.91 Pulling 1ml of CSR.lysis @15:09:32.92 CSR.select :lysis @15:09:33.03 CS.reconfigure CSconfig @15:09:33.08 CS.seek 1ml @15:09:36.84 delay 5 seconds @15:09:41.86 CSR.select :air @15:09:42.05 CS.seek 6ml @15:09:55.84 delay 2 seconds @15:09:57.85 CSV.dial :waste1 @15:09:59.12 CS.reconfigure CSfast @15:09:59.17 CS.seek :empty @15:10:13.08 CSV.dial CSR @15:10:14.41 Pulling 1.5ml of CSR.lysis @15:10:14.42 CSR.select :lysis @15:10:14.53 CS.reconfigure CSconfig @15:10:14.58 CS.seek 1.5ml @15:10:19.40 delay 5 seconds @15:10:24.42 CSR.select :air @15:10:24.61 CS.seek 4.5ml @15:10:33.28 delay 2 seconds @15:10:35.30 CSV.dial CTV @15:10:35.76 CS.seek 1.275ml @15:10:45.18 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @15:10:45.21 CH.seek 85C,8:00 @15:13:20.86 CH.hold 5:00,85C @15:15:05.31 GoodNews.email "At 14:00:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tundeployv2.rb:115\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "Started 18tundeployv2 phase mbaPhase" @15:15:45.42 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:15:45.43 Retry #8 of 10 after 10:00 @15:18:21.03
Collection Puck Heater at 85C after lysing @15:18:21.05 CS.seek 0ml @15:18:25.31 CTV.dial between spare and air,avoiding: :intake @15:18:26.34 CSV.dial :air @15:18:26.79 CS.seek 5ml @15:18:40.49 CSV.dial CTV @15:18:40.95 delay 2 seconds @15:18:42.96 CSV.dial :air @15:18:43.44 delay 2 seconds @15:18:45.46 CSV.dial CTV @15:18:45.92 delay 2 seconds @15:18:47.93 CSV.dial :air @15:18:48.41 delay 2 seconds @15:18:50.43 CS.seek 0ml @15:19:04.13 Recovering Lysate into Collection syringe @15:19:04.14 CSV.dial CBV @15:19:05.22 CBV.dial CSV @15:19:05.60 CS.seek 5ml @15:19:19.31 delay 5 seconds @15:19:24.33 CTV.dial :air @15:19:24.88 CS.seek 7ml @15:19:31.03 delay 5 seconds @15:19:36.05 CSV.dial :air2 @15:19:36.52 CS.seek 10ml @15:19:45.13 CSV.dial between air and CTV,avoiding: CBV @15:19:46.25 Lysing Complete @15:19:46.36 Storing sh1 puck in Collection Clamp to tube 1 @15:19:46.59 SC.reconfigure SCtight @15:19:46.64 SC.seek 1 @15:19:48.75 SC.reconfigure SCloose @15:19:48.98 Elbow.reconfigure TightElbow @15:19:49.03 Elbow.seek 1 @15:19:50.95 Forearm.reconfigure TightForearm @15:19:51.00 Forearm.seek 1 + 700 counts @15:19:52.05 SE.reconfigure SEpick @15:19:52.12 SE.seek beamBreak @15:19:54.46 SE.configure SEconfig @15:19:54.63 Elbow.reconfigure LimpElbow @15:19:54.69 Forearm.reconfigure LooseForearm @15:19:54.73 Forearm.seek 1 @15:19:55.06 Elbow.hold @15:19:55.13 Elbow.reconfigure TightElbow @15:19:55.24 SE.seek 8.09 Pucks below Top Plate @15:19:55.79 Forearm.force -20 @15:19:55.85 Forearm.seek 1 + 337 counts @15:19:56.17 Forearm.reconfigure TightForearm @15:19:56.21 Forearm.seek 1 + 700 counts @15:19:56.51 SE.seek 9.09 Pucks below Top Plate @15:19:57.19 Forearm.reconfigure LooseForearm @15:19:57.24 Forearm.seek :retract @15:19:58.14 Elbow.seek CC @15:19:59.78 Elbow.reconfigure LooseElbow @15:19:59.89 Forearm.seek CC @15:20:00.87 Hand.close @15:20:01.47 CC.seek :open @15:20:05.95 Forearm.seek :retract @15:20:06.91 Elbow.reconfigure TightElbow @15:20:06.96 Elbow.seek 1 @15:20:08.85 Elbow.reconfigure LimpElbow @15:20:08.90 Forearm.seek 1 @15:20:09.87 Elbow.hold @15:20:09.94 Elbow.reconfigure TightElbow @15:20:10.52 Hand.open @15:20:11.35 Forearm.force -20 @15:20:11.40 Forearm.seek 1 + 348 counts @15:20:11.72 Forearm.reconfigure TightForearm @15:20:11.77 Forearm.seek 1 + 700 counts @15:20:12.17 SE.seek :down @15:20:13.34
Forearm.reconfigure LooseForearm @15:20:13.39 Forearm.seek :retract @15:20:14.15 SE.coast @15:20:14.37
SC.coast @15:20:14.41 Tranferring lysate to PRV @15:20:14.42 CSV.dial :air @15:20:14.73 CS.seek 10ml @15:20:14.81 CSV.dial between air and CTV @15:20:15.13 AIV.dial :bypass @15:20:15.33 Beginning samplePassC @15:20:15.34 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:20:15.42 Hand.open @15:20:15.86 Elbow.seek FlushPuck::Garage @15:20:17.95 Elbow.reconfigure LooseElbow @15:20:18.07 Forearm.seek :garage @15:20:19.03 Hand.close @15:20:19.72 Forearm.seek :retract @15:20:20.70 Elbow.reconfigure TightElbow @15:20:20.75 Elbow.seek CC @15:20:23.61 Elbow.reconfigure LooseElbow @15:20:23.72 Forearm.reconfigure TightForearm @15:20:23.77 Forearm.seek CC @15:20:25.05 CC.seek :closed @15:20:29.35 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 1.76 @15:20:29.44 Hand.open @15:20:30.16 Forearm.reconfigure LooseForearm @15:20:30.20 Forearm.seek :retract @15:20:31.16 AWV.select :air @15:20:31.18 AIV.dial :bypass @15:20:31.19 PSV.dial PSR @15:20:32.53 Pulling 1.5ml of PSR.flush @15:20:32.54 PSR.select :flush @15:20:32.67 PS.seek 1.5ml @15:20:48.69 delay 5 seconds @15:20:53.71 PSV.dial :waste1 @15:20:54.19 PS.seek 0ml @15:21:10.28 PSV.dial PSR @15:21:10.75 PSR.select :air @15:21:10.88 PS.seek 2.5ml @15:21:37.10 PRV.dial CBV @15:21:37.13
CBV.dial PRV @15:21:38.52 PSV.dial :mixloop @15:21:39.54 CTV.dial :air,avoiding: :intake @15:21:39.55 PTV.dial :PRVmixing @15:21:39.64 PS.seek 0ml @15:22:05.74 PSV.dial :air @15:22:06.19 PS.seek 2.5ml @15:22:32.43 PSV.dial :mixloop @15:22:32.88 PS.seek 0ml @15:22:59.06 PSV.dial :air @15:22:59.54 PS.seek 2.5ml @15:23:25.71 PSV.dial :mixloop @15:23:26.18 PS.seek 0ml @15:23:52.26 Garaging FlushPuck in Collection Clamp @15:23:52.34 Elbow.reconfigure TightElbow @15:23:52.39 Elbow.seek CC @15:23:52.63 Elbow.reconfigure LooseElbow @15:23:52.74 Forearm.seek CC @15:23:53.68 Hand.close @15:23:54.35 CC.seek :open @15:23:58.76 Forearm.seek :retract @15:23:59.76 Elbow.reconfigure TightElbow @15:23:59.81 Elbow.seek :garage @15:24:02.89 Elbow.reconfigure LooseElbow @15:24:03.01 Forearm.reconfigure TightForearm @15:24:03.05 Forearm.seek :garage @15:24:04.23 Hand.open @15:24:04.92 Forearm.reconfigure LooseForearm @15:24:04.97 Forearm.seek :retract @15:24:06.01 Connecting PS to CS to transfer sample @15:24:06.02 CBV.dial AIV @15:24:06.03 AIV.dial :bypass @15:24:06.04 PSV.dial :mixloop @15:24:06.06 PTV.dial :PRVmixing @15:24:06.07 PRV.dial AIV @15:24:06.08 CTV.dial :air,avoiding: :intake @15:24:06.10 Loading lyfil puck from tube 2 into Collection Clamp @15:24:06.37 SC.reconfigure SCtight @15:24:06.42 SC.seek 2 @15:24:08.57 SC.reconfigure SCloose @15:24:08.79 Elbow.reconfigure TightElbow @15:24:08.84 Elbow.seek 2 @15:24:10.99 Forearm.reconfigure TightForearm @15:24:11.04 Forearm.seek 2 + 700 counts @15:24:12.08 SE.reconfigure SEpick @15:24:12.16 SE.seek beamBreak @15:24:14.10 SE.configure SEconfig @15:24:14.28 Elbow.seek 2 @15:24:14.47 Forearm.seek 2 + 300 counts @15:24:14.66 Forearm.reconfigure LooseForearm @15:24:14.70 Hand.close @15:24:15.36 SE.seek :down @15:24:15.60
Forearm.seek :retract @15:24:16.55 Elbow.seek CC @15:24:17.18 SE.coast @15:24:18.24
Elbow.reconfigure LooseElbow @15:24:18.35 Forearm.reconfigure TightForearm @15:24:18.40 Forearm.seek CC @15:24:19.61 CC.seek :closed @15:24:23.88 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.82 @15:24:23.89 Hand.open @15:24:24.54 Forearm.reconfigure LooseForearm @15:24:24.59 Forearm.seek :retract @15:24:25.56 SC.coast @15:24:25.60 CTV.dial CSV,avoiding: :intake @15:24:26.91 CSV.dial CTV @15:24:27.22 CS.seek 5ml @15:24:41.07 delay 1:20 @15:25:45.46 GoodNews.email "At 14:00:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tundeployv2.rb:115\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "Started 18tundeployv2 phase mbaPhase" @15:26:25.57 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:26:25.58 Retry #9 of 10 after 10:00 @15:26:25.61
CS.seek 2.5ml @15:26:32.95 delay 40 seconds @15:27:12.97 CS.seek 0ml @15:27:20.41 delay 1:00 @15:28:20.43 PSV.dial :air @15:28:20.90 PS.seek 2.5ml @15:28:47.00 PSV.dial :mixloop @15:28:47.46 delay 3 seconds @15:28:50.47 PSV.dial :air @15:28:50.95 delay 2 seconds @15:28:52.96 PSV.dial :mixloop @15:28:53.43 delay 3 seconds @15:28:56.45 PSV.dial :waste1 @15:28:57.74 PS.seek :empty @15:29:23.91 CBV.dial :waste1 @15:29:24.40 delay 5 seconds @15:29:29.42 CBV.dial AIV @15:29:29.90 delay 10 seconds @15:29:39.93 CBV.dial :waste1 @15:29:40.40 delay 10 seconds @15:29:50.42 CBV.dial AIV @15:29:50.90 delay 10 seconds @15:30:00.93 CTV.dial CSV,avoiding: :intake @15:30:00.94 CBV.dial between AIV and waste1 @15:30:01.28 CSV.dial :air @15:30:01.74 CS.seek 10ml @15:30:28.28 CSV.dial CTV @15:30:28.74 CS.seek 0ml @15:30:55.28 CBV.dial :waste1 @15:30:55.61 delay 10 seconds @15:31:05.64 CTV.dial :air,avoiding: :intake @15:31:06.99 Storing lyfil puck in Collection Clamp to tube 1 @15:31:07.18 SC.reconfigure SCtight @15:31:07.22 SC.seek 1 @15:31:09.33 SC.reconfigure SCloose @15:31:09.56 Elbow.reconfigure TightElbow @15:31:09.61 Elbow.seek 1 @15:31:11.49 Forearm.reconfigure TightForearm @15:31:11.54 Forearm.seek 1 + 700 counts @15:31:12.57 SE.reconfigure SEpick @15:31:12.63 SE.seek beamBreak @15:31:14.80 SE.configure SEconfig @15:31:14.96 Elbow.reconfigure LimpElbow @15:31:15.54 Forearm.reconfigure LooseForearm @15:31:15.62 Forearm.seek 1 @15:31:15.95 Elbow.hold @15:31:16.02 Elbow.reconfigure TightElbow @15:31:16.13 SE.seek 9.08 Pucks below Top Plate @15:31:16.64 Forearm.force -20 @15:31:16.68 Forearm.seek 1 + 327 counts @15:31:17.00 Forearm.reconfigure TightForearm @15:31:17.05 Forearm.seek 1 + 700 counts @15:31:17.34 SE.seek 10.08 Pucks below Top Plate @15:31:18.01 Forearm.reconfigure LooseForearm @15:31:18.06 Forearm.seek :retract @15:31:19.00 Elbow.seek CC @15:31:20.67 Elbow.reconfigure LooseElbow @15:31:20.78 Forearm.seek CC @15:31:21.78 Hand.close @15:31:22.36 CC.seek :open @15:31:26.87 Forearm.seek :retract @15:31:27.86 Elbow.reconfigure TightElbow @15:31:27.91 Elbow.seek 1 @15:31:29.82 Elbow.reconfigure LimpElbow @15:31:29.87 Forearm.seek 1 @15:31:30.88 Elbow.hold @15:31:30.95 Elbow.reconfigure TightElbow @15:31:31.00 Hand.open @15:31:31.74 Forearm.force -20 @15:31:31.78 Forearm.seek 1 + 350 counts @15:31:32.10 Forearm.reconfigure TightForearm @15:31:32.15 Forearm.seek 1 + 700 counts @15:31:32.47 SE.seek :down @15:31:33.62
Forearm.reconfigure LooseForearm @15:31:33.66 Forearm.seek :retract @15:31:34.24 SE.coast @15:31:34.55
SC.coast @15:31:34.59 PSV.dial :air @15:31:35.78 PS.seek 0.499ml @15:31:41.64 delay 3 seconds @15:31:44.65 PSV.dial :mixloop @15:31:45.11 PRV.dial :air @15:31:45.58 delay 3 seconds @15:31:48.61 PRV.dial CBV @15:31:49.08 CBV.dial AIV @15:31:49.55 PRV.dial between CBV and air @15:31:49.90 PRV.dial :air @15:31:50.27 PS.reconfigure PSslow @15:31:50.32 PS.seek 0.599ml @15:31:52.84 PRV.dial :waste2 @15:31:53.57 PS.reconfigure PSconfig @15:31:53.62 PS.seek :empty @15:32:00.51 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:32:00.58 Hand.open @15:32:01.46 Elbow.seek FlushPuck::Garage @15:32:03.50 Elbow.reconfigure LooseElbow @15:32:03.61 Forearm.seek :garage @15:32:04.64 Hand.close @15:32:05.37 Forearm.seek :retract @15:32:06.38 Elbow.reconfigure TightElbow @15:32:06.43 Elbow.seek CC @15:32:09.39 Elbow.reconfigure LooseElbow @15:32:09.50 Forearm.reconfigure TightForearm @15:32:09.55 Forearm.seek CC @15:32:10.81 CC.seek :closed @15:32:15.07 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.83 @15:32:15.14 Hand.open @15:32:15.81 Forearm.reconfigure LooseForearm @15:32:15.86 Forearm.seek :retract @15:32:16.91 Clearing PML for modified lysate @15:32:16.92 CTV.dial :air,avoiding: :intake @15:32:16.93 PRV.dial :waste2 @15:32:16.95 PTV.dial :PRV,avoiding: :puck @15:32:17.68 delay 2 seconds @15:32:19.71 PTV.dial :PRVmixing,avoiding: :puck @15:32:20.45 delay 2 seconds @15:32:22.46 PSV.dial :PML @15:32:22.48 delay 2 seconds @15:32:24.50 PRV.dial :air @15:32:25.24 delay 2 seconds @15:32:27.32 PS.seek 1.499ml @15:32:43.32 delay 5 seconds @15:32:48.35 PS.seek 2.5ml @15:32:59.38 PSV.dial :air @15:32:59.86 PS.seek 0ml @15:33:25.98 mixing lysate with speDil @15:33:25.99 PSV.dial :PML @15:33:26.44 CBV.dial :PRV @15:33:26.46 PRV.dial :air @15:33:26.47 delay 2 seconds @15:33:28.49 PRV.dial :speDil @15:33:28.98 delay 2 seconds @15:33:31.07 PS.reconfigure PSslow1 @15:33:31.12 PS.seek 0.225ml @15:33:54.92 delay 5 seconds @15:33:59.95 PRV.dial CBV @15:34:00.70 delay 2 seconds @15:34:02.78 PS.seek 0.475ml @15:34:28.57 delay 5 seconds @15:34:33.59 PRV.dial :air @15:34:34.07 delay 2 seconds @15:34:36.09 PS.seek 0.965ml @15:35:26.04 delay 10 seconds @15:35:36.06 PRV.dial CBV @15:35:36.53 CTV.dial :air,avoiding: :intake @15:35:36.54 PSV.dial :air @15:35:37.00 PS.reconfigure PSconfig @15:35:37.05 PS.seek 2.5ml @15:35:53.36 delay 3 seconds @15:35:56.37 PSV.dial PTV @15:35:57.11 PTV.dial :PRV,avoiding: :puck @15:35:57.84 PS.seek 0ml @15:36:24.44 PSV.dial :air @15:36:25.20 PS.seek 2.5ml @15:36:25.61 GoodNews.email "At 14:00:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tundeployv2.rb:115\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "Started 18tundeployv2 phase mbaPhase" @15:37:05.72 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:37:05.78
delay 3 seconds @15:37:05.80 Retry #10 of 10 after 10:00 @15:37:08.80
PSV.dial PTV @15:37:09.53 PTV.dial :PRV,avoiding: :puck @15:37:09.55 PS.seek 0ml @15:37:35.67 Pushing modified lysate into AIV Injection Loop @15:37:35.68 CBV.dial AIV @15:37:35.69 AIV.dial :bypass @15:37:35.70 PRV.dial CBV @15:37:35.72 PSV.dial :air @15:37:36.45 PS.seek 2.5ml @15:38:02.58 delay 3 seconds @15:38:05.59 PSV.dial :PML @15:38:06.05 PTV.dial :PRVmixing,avoiding: :puck @15:38:06.85 PS.reconfigure PSslow1 @15:38:06.90 PS.seek 1.74ml @15:39:24.30 delay 5 seconds @15:39:29.32 AIV.dial :sample @15:39:29.88 AWV.select :waste @15:39:30.06 PS.reconfigure PSslow2 @15:39:30.11 PS.seek 1.22ml @15:41:18.52 delay 15 seconds @15:41:33.54 AIV.dial :bypass @15:41:34.11 PS.reconfigure PSconfig @15:41:34.16 PS.seek 0ml @15:41:47.38 PSV.dial :air @15:41:47.83 PS.seek 2.5ml @15:42:13.98 PSV.dial :PML @15:42:14.44 PS.seek 0ml @15:42:40.60 AIV.dial :coilToMFB @15:42:40.62 AWV.select :air @15:42:40.78 Extracting Nucleic Acid from AIV @15:42:40.82
Beginning samplePassD @15:42:40.85 SPE.hold 2:46:40,55C @15:42:40.93 ASV.dial :SPEdelivery @15:42:41.02
AWV.select :air @15:42:41.03 AIV.dial :bypass @15:42:41.04 PSV.dial PSR @15:42:41.32 delay 20 seconds @15:42:42.15
Pulling 1.5ml of PSR.killMFB @15:42:42.16 PSR.select :killMFB @15:42:42.29 PS.seek 1.5ml @15:42:58.27 delay 5 seconds @15:43:01.35 primeLysateINT @15:43:01.36 AWV.select :air @15:43:01.37 AIV.dial :coilToMFB @15:43:01.38 ATV.dial AIV @15:43:02.99 AS.reconfigure ASslow1 @15:43:03.04 AS.seek 0.299ml @15:43:03.28
PSV.dial :waste1 @15:43:03.77 PS.seek 0ml @15:43:19.85 PSV.dial PSR @15:43:20.44 PSR.select :air @15:43:20.57 PS.seek 2.5ml @15:43:46.67 PRV.dial CBV @15:43:46.70
CBV.dial PRV @15:43:46.71 PSV.dial :mixloop @15:43:47.75 CTV.dial :air,avoiding: :intake @15:43:47.76 PTV.dial :PRVmixing @15:43:47.78 PS.seek 0ml @15:44:03.70 delay 10 seconds @15:44:13.72 ATV.dial :air2 @15:44:13.91
PSV.dial PSR @15:44:14.50 AS.seek 0.329ml @15:44:15.00
Pulling 1.5ml of PSR.flush @15:44:15.01 PSR.select :flush @15:44:15.14 PS.seek 1.5ml @15:44:20.80 ATV.dial :waste2 @15:44:21.43 AS.reconfigure ASnorm @15:44:21.48 AS.seek :empty @15:44:31.27
delay 5 seconds @15:44:36.29 PSV.dial :waste1 @15:44:36.78 PS.seek 0ml @15:44:38.48 Lysate primed! @15:44:38.49 AFV.select :holdingCoil @15:44:38.50 ATV.dial :air2 @15:44:39.08 AS.reconfigure ASslow1 @15:44:39.13 AS.seek 0.069ml @15:44:53.48 delay 3 seconds @15:44:53.63
PSV.dial PSR @15:44:54.11 PSR.select :air @15:44:54.24 PS.seek 2.5ml @15:44:56.50 ATV.dial :AIV @15:44:57.28 AS.seek 0.469ml @15:45:20.33 PRV.dial CBV @15:45:20.36
CBV.dial PRV @15:45:20.38 PSV.dial :mixloop @15:45:21.41 CTV.dial :air,avoiding: :intake @15:45:21.42 PTV.dial :PRVmixing @15:45:21.44 PS.seek 0ml @15:45:47.54 PSV.dial PSR @15:45:48.63 Pulling 1.5ml of PSR.flush @15:45:48.64 PSR.select :flush @15:45:48.77 PS.seek 1.5ml @15:46:04.80 delay 5 seconds @15:46:09.82 PSV.dial :waste1 @15:46:10.28 PS.seek 0ml @15:46:18.00 delay 5 seconds @15:46:23.08 Solid Phase Extraction Heater at 50.8C @15:46:23.09 ATV.dial ASV @15:46:23.56 ASV.dial :SPEdelivery @15:46:23.65 AS.seek 0.041ml @15:46:26.26
PSV.dial PSR @15:46:26.73 PSR.select :air @15:46:26.87 PS.seek 2.5ml @15:46:53.08 PRV.dial CBV @15:46:53.11
CBV.dial PRV @15:46:53.13 PSV.dial :mixloop @15:46:54.14 CTV.dial :air,avoiding: :intake @15:46:54.15 PTV.dial :PRVmixing @15:46:54.17 PS.seek 0ml @15:47:05.83 GoodNews.email "At 14:00:23 began in tube 2: mbaPhase\n/home/esp/esp2/mission/18tundeployv2.rb:115\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "Started 18tundeployv2 phase mbaPhase" @15:47:45.94 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:47:45.98
PSV.dial PSR @15:47:46.00 BACfans.email "Started mfb in tube 2 at 14:05:14CEST03-Oct-18\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @15:48:26.13 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:48:26.19 Retry #1 of 10 after 10:00 @15:48:26.23 delay 10 seconds @15:48:27.22
Pulling 1.5ml of PSR.flush @15:48:27.23 PSR.select :flush @15:48:27.36 PS.seek 1.5ml @15:48:36.27 rinseLysateLineINT @15:48:36.28 AFV.select :carrierSolution @15:48:36.39 delay 2 seconds @15:48:38.42 AS.reconfigure ASconfig @15:48:38.47 AS.seek 1ml @15:48:43.43
delay 5 seconds @15:48:48.45 PSV.dial :waste1 @15:48:48.93 PS.seek 0ml @15:49:05.23 PSV.dial PSR @15:49:05.82 PSR.select :air @15:49:05.95 PS.seek 2.5ml @15:49:32.05 PRV.dial CBV @15:49:32.08
CBV.dial PRV @15:49:32.09 PSV.dial :mixloop @15:49:33.10 CTV.dial :air,avoiding: :intake @15:49:33.11 PTV.dial :PRVmixing @15:49:33.13 PS.reconfigure PSslow @15:49:33.18 PS.seek 1.2ml @15:49:33.89 delay 1 second @15:49:34.90 AFV.select :holdingCoil @15:49:35.01 delay 1 second @15:49:36.02 ATV.dial AIV @15:49:36.53 AIV.dial :coilToMFB @15:49:36.54 AWV.select :waste @15:49:36.65 AS.reconfigure ASslow0 @15:49:36.70 AS.seek 0ml @15:49:59.99
PSV.dial :waste1 @15:50:01.30 PS.reconfigure PSconfig @15:50:01.35 PS.seek 0ml @15:50:14.42 PSV.dial :air @15:50:15.44 PS.seek 2.5ml @15:50:41.51 PSV.dial :mixloop @15:50:41.96 PS.reconfigure PSslow @15:50:42.01 PS.seek 0ml @15:51:17.60 ATV.dial :air2 @15:51:18.26 AS.reconfigure ASslow1 @15:51:18.31 AS.seek 0.05ml @15:51:28.66 ATV.dial :AIV @15:51:29.46 AS.seek 0.01ml @15:51:33.61
PSV.dial :air @15:51:34.09 PS.reconfigure PSconfig @15:51:34.14 PS.seek 2.5ml @15:51:37.79 ATV.dial :waste @15:51:39.42 AS.seek :empty @15:51:41.87 ethanol wash @15:51:41.88 ATV.dial :air2 @15:51:43.05 AS.reconfigure ASslow2 @15:51:43.10 AS.seek 0.009ml @15:51:53.50 delay 3 seconds @15:51:56.53 Pulling 0.4ml of Ethanol Wash @15:51:56.54 ATV.dial :ethanol @15:51:57.33 AS.reconfigure ASslow1 @15:51:57.38 AS.seek 0.409ml @15:52:00.17
PSV.dial :mixloop @15:52:00.65 PS.reconfigure PSslow @15:52:00.70 PS.seek 0ml @15:52:51.74 Cleaning Collection Syringe @15:52:51.75 CSV.dial CSR @15:52:52.26 Pulling 4ml of CSR.flush @15:52:52.27 CSR.select :flush @15:52:52.40 CS.seek 4ml @15:53:03.59 delay 5 seconds @15:53:08.61 CSR.select :air @15:53:08.75 CS.seek 10ml @15:53:18.08 delay 3 seconds @15:53:21.10 ATV.dial :air1 @15:53:21.47 delay 3 seconds @15:53:24.55 AS.seek 0.434ml @15:53:25.03 CSV.dial CTV @15:53:25.06
CTV.dial CSV @15:53:26.44 CBV.dial :waste1 @15:53:26.92 CS.seek 0ml @15:53:29.87 delay 3 seconds @15:53:32.89 ATV.dial :ethanol @15:53:33.49 AS.seek 0.419ml @15:53:36.78 delay 3 seconds @15:53:39.79 ATV.dial ASV @15:53:41.05 AS.seek 0.092ml @15:53:53.46
CSV.dial CSR @15:53:54.00 Pulling 4ml of CSR.flush @15:53:54.01 CSR.select :flush @15:53:54.14 CS.seek 4ml @15:54:05.31 delay 5 seconds @15:54:10.33 CSR.select :air @15:54:10.46 CS.seek 10ml @15:54:26.72 CSV.dial CTV @15:54:26.75
CTV.dial CSV @15:54:27.22 CBV.dial :waste1 @15:54:27.24 CS.seek 0ml @15:54:47.09 delay 3 seconds @15:54:50.11 ATV.dial :waste2 @15:54:50.83 AS.reconfigure ASconfig @15:54:50.88 AS.seek :empty @15:54:53.75
Garaging FlushPuck in Collection Clamp @15:54:53.84 Elbow.reconfigure TightElbow @15:54:53.89 Elbow.seek CC @15:54:54.14 Elbow.reconfigure LooseElbow @15:54:54.25 Forearm.seek CC @15:54:55.24 Hand.close @15:54:55.89 CC.seek :open @15:54:56.60 ATV.dial :air2 @15:54:56.98 AS.reconfigure ASslow1 @15:54:57.03 AS.seek 1ml @15:55:00.26
Forearm.seek :retract @15:55:01.82 Elbow.reconfigure TightElbow @15:55:01.90 Elbow.seek :garage @15:55:04.97 Elbow.reconfigure LooseElbow @15:55:05.08 Forearm.reconfigure TightForearm @15:55:05.14 Forearm.seek :garage @15:55:06.31 Hand.open @15:55:06.96 Forearm.reconfigure LooseForearm @15:55:07.01 Forearm.seek :retract @15:55:08.03 Whole Cell Archive sampling 1500ml @15:55:08.05 Loading wcr puck from tube 2 into Collection Clamp @15:55:08.32 SC.reconfigure SCtight @15:55:08.37 SC.seek 2 @15:55:10.53 SC.reconfigure SCloose @15:55:10.77 Elbow.reconfigure TightElbow @15:55:10.82 Elbow.seek 2 @15:55:12.92 Forearm.reconfigure TightForearm @15:55:12.97 Forearm.seek 2 + 700 counts @15:55:13.99 SE.reconfigure SEpick @15:55:14.06 SE.seek beamBreak @15:55:16.11 SE.configure SEconfig @15:55:16.28 Elbow.seek 2 @15:55:16.58 Forearm.seek 2 + 300 counts @15:55:16.85 Forearm.reconfigure LooseForearm @15:55:16.90 Hand.close @15:55:17.54 SE.seek :down @15:55:17.77 Forearm.seek :retract @15:55:18.74 Elbow.seek CC @15:55:19.43 SE.coast @15:55:20.46 Elbow.reconfigure LooseElbow @15:55:20.57 Forearm.reconfigure TightForearm @15:55:20.62 Forearm.seek CC @15:55:21.93 CC.seek :closed @15:55:26.19 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.83 @15:55:26.21 Hand.open @15:55:26.97 Forearm.reconfigure LooseForearm @15:55:27.02 Forearm.seek :retract @15:55:27.97 SC.coast @15:55:28.01 collectSample 1500ml w/bubble point of 55psi @15:55:28.03 Priming 7ml Intake.select :close @15:55:28.04 Exhaust.select :close @15:55:28.06 SS.hold @15:55:28.29 Calibrating pressure sensors @15:55:28.30 CTV.dial :air @15:55:29.60 SSV.dial :spit,avoiding: :exhaust @15:55:30.22 SS.reconfigure SSfast @15:55:30.27 SS.seek 5ml @15:55:32.90 SSV.dial CBV @15:55:32.93 CBV.dial SSV @15:55:34.31 delay 3.8 seconds @15:55:38.26 SSV.dial :spit @15:55:39.58 SS.seek 0ml @15:55:42.44 SSV.dial CBV @15:55:43.78 CTV.dial :intake @15:55:44.53 SSV.dial CBV @15:55:44.56 CBV.dial SSV @15:55:44.58 Allowing 40s for 7.0ml of remaining 7.0ml @15:55:44.60 Intake.select :open @15:55:46.77 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:55:46.80 SS.reconfigure SSinhale.with maxSpeed: 150 @15:55:46.84 SS.seek 7ml @15:55:50.38 Vacuum too high -- waiting up to 40s @15:55:52.78 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:55:52.80 SS.reconfigure SSinhale.with maxSpeed: 135 @15:55:52.85 SS.seek 7ml @15:55:55.98 Stroke completed -- waiting for pressure to equalize ... @15:56:01.13 Intake.select :close @15:56:01.29 SSV.dial :exhaust @15:56:02.06 Exhaust.select :open @15:56:02.18 SS.reconfigure SSexhale @15:56:02.23 SS.seek 0ml @15:56:06.07 Exhaust.select :close @15:56:06.19 Sampling 1500.0ml @15:56:06.20 Intake.select :close @15:56:06.22 Exhaust.select :close @15:56:06.23 SS.reconfigure SSconfig @15:56:06.28 SS.hold @15:56:06.50 CTV.dial :intake @15:56:06.52 SSV.dial CBV @15:56:06.55 CBV.dial SSV @15:56:07.34 Allowing 130s for 25.0ml of remaining 1500.0ml @15:56:07.35 Intake.select :open @15:56:09.53 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:56:09.55 SS.reconfigure SSinhale.with maxSpeed: 135 @15:56:09.60 SS.seek 25ml @15:56:13.16 Vacuum too high -- waiting up to 40s @15:56:15.05 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:56:15.07 SS.reconfigure SSinhale.with maxSpeed: 122 @15:56:15.72 SS.seek 25ml @15:56:18.86 Vacuum too high -- waiting up to 40s @15:56:20.49 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:56:20.51 SS.reconfigure SSinhale.with maxSpeed: 110 @15:56:20.56 SS.seek 25ml @15:56:23.19 Vacuum too high -- waiting up to 40s @15:56:24.84 SS.reconfigure SSinhale.with maxSpeed: 99 @15:56:24.90 SS.seek 25ml @15:56:27.63 Vacuum too high -- waiting up to 40s @15:56:29.02 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:56:29.04 SS.reconfigure SSinhale.with maxSpeed: 89 @15:56:29.09 SS.seek 25ml @15:56:31.79 Vacuum too high -- waiting up to 40s @15:56:33.16 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:56:33.18 SS.reconfigure SSinhale.with maxSpeed: 80 @15:56:33.23 SS.seek 25ml @15:56:36.44 Vacuum too high -- waiting up to 40s @15:56:37.81 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:56:37.83 SS.reconfigure SSinhale.with maxSpeed: 72 @15:56:37.88 SS.seek 25ml @15:56:41.55 Vacuum too high -- waiting up to 40s @15:56:42.63 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:56:42.65 SS.reconfigure SSinhale.with maxSpeed: 65 @15:56:42.70 SS.seek 25ml @15:56:47.06 Stroke completed -- waiting for pressure to equalize ... @15:56:50.96 Intake.select :close @15:56:51.12 SSV.dial :exhaust @15:56:51.88 Exhaust.select :open @15:56:52.59 SS.reconfigure SSexhale @15:56:52.67 SS.seek 0ml @15:57:01.69 Exhaust.select :close @15:57:01.81 SSV.dial CBV @15:57:01.84 CBV.dial SSV @15:57:02.63 Allowing 130s for 25.0ml of remaining 1475.0ml @15:57:02.64 Intake.select :open @15:57:04.83 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:57:04.85 SS.reconfigure SSinhale.with maxSpeed: 65 @15:57:04.90 SS.seek 25ml @15:57:10.75 Vacuum too high -- waiting up to 40s @15:57:12.11 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:57:12.13 SS.reconfigure SSinhale.with maxSpeed: 59 @15:57:12.18 SS.seek 25ml @15:57:19.25 Vacuum too high -- waiting up to 40s @15:57:20.37 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:57:20.39 SS.reconfigure SSinhale.with maxSpeed: 53 @15:57:20.44 SS.seek 25ml @15:57:39.75 Vacuum too high -- waiting up to 40s @15:57:40.87 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:57:40.89 SS.reconfigure SSinhale.with maxSpeed: 48 @15:57:40.94 SS.seek 25ml @15:57:41.72 Stroke completed -- waiting for pressure to equalize ... @15:57:43.69 Intake.select :close @15:57:43.86 SSV.dial :exhaust @15:57:44.62 Exhaust.select :open @15:57:44.75 SS.reconfigure SSexhale @15:57:44.82 SS.seek 0ml @15:57:53.76 Exhaust.select :close @15:57:53.88 SSV.dial CBV @15:57:53.91 CBV.dial SSV @15:57:54.70 Allowing 130s for 25.0ml of remaining 1450.0ml @15:57:54.71 Intake.select :open @15:57:56.90 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:57:56.92 SS.reconfigure SSinhale.with maxSpeed: 48 @15:57:56.96 SS.seek 25ml @15:58:18.64 delay 5 seconds @15:58:23.66 ATV.dial ASV @15:58:24.59 ASV.dial :SPEdelivery @15:58:24.61 AS.seek 0.02ml @15:58:26.22 BACfans.email "Started mfb in tube 2 at 14:05:14CEST03-Oct-18\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @15:59:06.33 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:59:06.36 Stroke completed -- waiting for pressure to equalize ... @15:59:06.39 Retry #2 of 10 after 10:00 @15:59:07.08 Intake.select :close @15:59:07.24 SSV.dial :exhaust @15:59:08.01 Exhaust.select :open @15:59:08.12 SS.reconfigure SSexhale @15:59:08.17 SS.seek 0ml @15:59:17.12 Exhaust.select :close @15:59:17.24 SSV.dial CBV @15:59:17.27 CBV.dial SSV @15:59:18.06 Allowing 130s for 25.0ml of remaining 1425.0ml @15:59:18.07 Intake.select :open @15:59:20.26 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:59:20.27 SS.reconfigure SSinhale.with maxSpeed: 48 @15:59:20.33 SS.seek 25ml @15:59:57.25 Stroke completed -- waiting for pressure to equalize ... @15:59:59.80 Intake.select :close @15:59:59.97 SSV.dial :exhaust @16:00:00.74 Exhaust.select :open @16:00:00.85 SS.reconfigure SSexhale @16:00:00.91 SS.seek 0ml @16:00:09.95 Exhaust.select :close @16:00:10.07 SSV.dial CBV @16:00:10.10 CBV.dial SSV @16:00:10.90 Allowing 130s for 25.0ml of remaining 1400.0ml @16:00:10.91 Intake.select :open @16:00:13.09 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:00:13.11 SS.reconfigure SSinhale.with maxSpeed: 48 @16:00:13.16 SS.seek 25ml @16:00:50.33 Stroke completed -- waiting for pressure to equalize ... @16:00:53.57 Intake.select :close @16:00:53.73 SSV.dial :exhaust @16:00:54.49 Exhaust.select :open @16:00:54.60 SS.reconfigure SSexhale @16:00:54.66 SS.seek 0ml @16:01:03.59 Exhaust.select :close @16:01:03.71 SSV.dial CBV @16:01:03.74 CBV.dial SSV @16:01:04.52 Allowing 130s for 25.0ml of remaining 1375.0ml @16:01:04.53 Intake.select :open @16:01:06.72 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:01:06.74 SS.reconfigure SSinhale.with maxSpeed: 48 @16:01:06.79 SS.seek 25ml @16:01:42.05 delay 5 seconds @16:01:43.89 Stroke completed -- waiting for pressure to equalize ... @16:01:47.13 ATV.dial :air2 @16:01:47.20 Intake.select :close @16:01:47.36 SSV.dial :exhaust @16:01:48.13 AS.seek 0.52ml @16:01:48.19 Exhaust.select :open @16:01:48.31 SS.reconfigure SSexhale @16:01:48.36 SS.seek 0ml @16:01:57.67 Exhaust.select :close @16:01:57.79 SSV.dial CBV @16:01:57.82 CBV.dial SSV @16:01:58.62 Allowing 130s for 25.0ml of remaining 1350.0ml @16:01:58.63 Intake.select :open @16:02:00.81 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:02:00.83 SS.reconfigure SSinhale.with maxSpeed: 48 @16:02:00.88 SS.seek 25ml @16:02:38.09 Stroke completed -- waiting for pressure to equalize ... @16:02:41.34 Intake.select :close @16:02:41.50 SSV.dial :exhaust @16:02:42.26 Exhaust.select :open @16:02:42.37 SS.reconfigure SSexhale @16:02:42.42 SS.seek 0ml @16:02:51.19 Exhaust.select :close @16:02:51.31 SSV.dial CBV @16:02:51.34 CBV.dial SSV @16:02:52.13 Allowing 130s for 25.0ml of remaining 1325.0ml @16:02:52.14 Intake.select :open @16:02:54.33 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:02:54.35 SS.reconfigure SSinhale.with maxSpeed: 48 @16:02:54.40 SS.seek 25ml @16:03:29.05 delay 5 seconds @16:03:32.47 Stroke completed -- waiting for pressure to equalize ... @16:03:34.06 ATV.dial ASV @16:03:35.04 ASV.dial :wasteR @16:03:35.42 AS.seek 0.3ml @16:03:35.74 Intake.select :close @16:03:35.91 SSV.dial :exhaust @16:03:36.70 Exhaust.select :open @16:03:36.81 SS.reconfigure SSexhale @16:03:36.87 SS.seek 0ml @16:03:45.59 Exhaust.select :close @16:03:45.71 SSV.dial CBV @16:03:45.74 CBV.dial SSV @16:03:46.54 Allowing 130s for 25.0ml of remaining 1300.0ml @16:03:46.55 Intake.select :open @16:03:48.73 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:03:48.75 SS.reconfigure SSinhale.with maxSpeed: 48 @16:03:48.80 SS.seek 25ml @16:04:19.92 delay 20 seconds @16:04:26.23 Stroke completed -- waiting for pressure to equalize ... @16:04:29.47 Intake.select :close @16:04:29.64 SSV.dial :exhaust @16:04:30.41 Exhaust.select :open @16:04:30.52 SS.reconfigure SSexhale @16:04:30.57 SS.seek 0ml @16:04:39.54 Exhaust.select :close @16:04:39.66 SSV.dial CBV @16:04:39.69 CBV.dial SSV @16:04:39.95 AS.seek 0.07ml @16:04:40.48 Allowing 130s for 25.0ml of remaining 1275.0ml @16:04:40.49 Intake.select :open @16:04:42.67 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:04:42.69 SS.reconfigure SSinhale.with maxSpeed: 48 @16:04:42.74 SS.seek 25ml @16:05:19.89 Stroke completed -- waiting for pressure to equalize ... @16:05:23.76 Intake.select :close @16:05:23.96 SSV.dial :exhaust @16:05:24.76 Exhaust.select :open @16:05:24.87 SS.reconfigure SSexhale @16:05:24.93 SS.seek 0ml @16:05:26.45 delay 20 seconds @16:05:33.97 Exhaust.select :close @16:05:34.09 SSV.dial CBV @16:05:34.12 CBV.dial SSV @16:05:34.91 Allowing 130s for 25.0ml of remaining 1250.0ml @16:05:34.92 Intake.select :open @16:05:37.10 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:05:37.12 SS.reconfigure SSinhale.with maxSpeed: 48 @16:05:37.17 SS.seek 25ml @16:05:46.47 ATV.dial :waste2 @16:05:47.19 AS.reconfigure ASconfig @16:05:47.24 AS.seek :empty @16:05:51.64 Elution @16:05:51.65 ATV.dial :air1 @16:05:52.70 AS.reconfigure ASslow1 @16:05:52.75 AS.seek 0.049ml @16:06:03.08 delay 3 seconds @16:06:06.10 ATV.dial :ElutionWater @16:06:06.77 AS.seek 0.109ml @16:06:07.35 Vacuum too high -- waiting up to 40s @16:06:07.77 Intake @33.4psia, vacuum from 20.0 to 24.0psi @16:06:07.79 SS.reconfigure SSinhale.with maxSpeed: 43 @16:06:07.84 SS.seek 25ml @16:06:16.44 Stroke completed -- waiting for pressure to equalize ... @16:06:19.10 delay 3 seconds @16:06:19.67 Intake.select :close @16:06:19.84 SSV.dial :exhaust @16:06:20.62 Exhaust.select :open @16:06:20.73 SS.reconfigure SSexhale @16:06:20.79 SS.seek 0ml @16:06:22.13 ATV.dial ASV,via: :air2 @16:06:24.06 ASV.dial :SPEelution @16:06:24.60 AFV.select :carrierSolution @16:06:24.77 AS.reconfigure ASnorm @16:06:24.82 AS.seek 0.759ml @16:06:29.86 Exhaust.select :close @16:06:29.98 SSV.dial CBV @16:06:30.01 CBV.dial SSV @16:06:30.81 Allowing 130s for 25.0ml of remaining 1225.0ml @16:06:30.82 Intake.select :open @16:06:33.01 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:06:33.03 SS.reconfigure SSinhale.with maxSpeed: 43 @16:06:33.08 SS.seek 25ml @16:06:57.86 delay 3 seconds @16:07:00.88 AFV.select :holdingCoil @16:07:01.04 AS.reconfigure ASslow1 @16:07:01.09 AS.seek 0.474ml @16:07:14.12 Stroke completed -- waiting for pressure to equalize ... @16:07:17.36 Intake.select :close @16:07:17.52 SSV.dial :exhaust @16:07:18.29 Exhaust.select :open @16:07:18.40 SS.reconfigure SSexhale @16:07:18.45 SS.seek 0ml @16:07:27.15 Exhaust.select :close @16:07:27.27 SSV.dial CBV @16:07:27.30 CBV.dial SSV @16:07:28.09 Allowing 130s for 25.0ml of remaining 1200.0ml @16:07:28.10 Intake.select :open @16:07:30.28 Intake @33.5psia, vacuum from 20.0 to 24.0psi @16:07:30.30 SS.reconfigure SSinhale.with maxSpeed: 43 @16:07:30.35 SS.seek 25ml @16:07:58.66 delay 5 seconds @16:08:03.75 AS.reconfigure ASslow2 @16:08:03.81 AS.seek 0.439ml @16:08:11.95 Stroke completed -- waiting for pressure to equalize ... @16:08:15.18 Intake.select :close @16:08:15.34 SSV.dial :exhaust @16:08:16.12 Exhaust.select :open @16:08:16.23 SS.reconfigure SSexhale @16:08:16.28 SS.seek 0ml @16:08:25.34 Exhaust.select :close @16:08:25.46 SSV.dial CBV @16:08:25.49 CBV.dial SSV @16:08:26.27 Allowing 130s for 25.0ml of remaining 1175.0ml @16:08:26.28 Intake.select :open @16:08:28.47 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:08:28.49 SS.reconfigure SSinhale.with maxSpeed: 43 @16:08:28.53 SS.seek 25ml @16:08:39.21 delay 1:00 @16:09:06.42 BACfans.email "Started mfb in tube 2 at 14:05:14CEST03-Oct-18\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @16:09:46.54 Stroke completed -- waiting for pressure to equalize ... @16:09:47.15 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @16:09:47.18 Retry #3 of 10 after 10:00 @16:09:47.21 pushing eluate to holding coil @16:09:47.29 AS.seek 0.279ml @16:09:47.85 Intake.select :close @16:09:48.02 SSV.dial :exhaust @16:09:48.79 Exhaust.select :open @16:09:48.90 SS.reconfigure SSexhale @16:09:48.96 SS.seek 0ml @16:09:57.64 Exhaust.select :close @16:09:57.76 SSV.dial CBV @16:09:57.79 CBV.dial SSV @16:09:58.59 Allowing 130s for 25.0ml of remaining 1150.0ml @16:09:58.60 Intake.select :open @16:10:00.79 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:10:00.81 SS.reconfigure SSinhale.with maxSpeed: 43 @16:10:00.86 SS.seek 25ml @16:10:42.47 Stroke completed -- waiting for pressure to equalize ... @16:10:46.40 Intake.select :close @16:10:46.56 SSV.dial :exhaust @16:10:47.33 Exhaust.select :open @16:10:47.45 SS.reconfigure SSexhale @16:10:47.52 SS.seek 0ml @16:10:56.39 Exhaust.select :close @16:10:56.51 SSV.dial CBV @16:10:56.54 CBV.dial SSV @16:10:57.34 Allowing 130s for 25.0ml of remaining 1125.0ml @16:10:57.35 Intake.select :open @16:10:59.53 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:10:59.55 SS.reconfigure SSinhale.with maxSpeed: 43 @16:10:59.60 SS.seek 25ml @16:11:41.20 Stroke completed -- waiting for pressure to equalize ... @16:11:45.07 Intake.select :close @16:11:45.23 SSV.dial :exhaust @16:11:46.01 Exhaust.select :open @16:11:46.12 SS.reconfigure SSexhale @16:11:46.17 SS.seek 0ml @16:11:55.40 Exhaust.select :close @16:11:55.52 SSV.dial CBV @16:11:55.55 CBV.dial SSV @16:11:56.35 Allowing 130s for 25.0ml of remaining 1100.0ml @16:11:56.36 Intake.select :open @16:11:58.54 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:11:58.56 SS.reconfigure SSinhale.with maxSpeed: 43 @16:11:58.61 SS.seek 25ml @16:12:28.41 delay 30 seconds @16:12:40.01 Stroke completed -- waiting for pressure to equalize ... @16:12:43.89 Intake.select :close @16:12:44.05 SSV.dial :exhaust @16:12:44.82 Exhaust.select :open @16:12:44.93 SS.reconfigure SSexhale @16:12:44.98 SS.seek 0ml @16:12:53.94 Exhaust.select :close @16:12:54.06 SSV.dial CBV @16:12:54.09 CBV.dial SSV @16:12:55.49 Allowing 130s for 25.0ml of remaining 1075.0ml @16:12:55.50 Intake.select :open @16:12:57.68 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:12:57.70 SS.reconfigure SSinhale.with maxSpeed: 43 @16:12:57.75 SS.seek 25ml @16:12:58.43 ATV.dial :waste,via: :air3 @16:13:00.00 AS.reconfigure ASconfig @16:13:00.05 AS.seek :empty @16:13:16.46 Parking Bleach in LysateSource line and SPEcolumn @16:13:16.47 ATV.dial :air3 @16:13:17.26 AS.reconfigure ASslow1 @16:13:17.31 AS.seek 0.029ml @16:13:23.65 ATV.dial :ElutionWater @16:13:24.23 AS.seek 0.059ml @16:13:30.51 ATV.dial :air3 @16:13:31.21 AS.seek 0.069ml @16:13:33.49 delay 2 seconds @16:13:35.50 ATV.dial :bleach @16:13:35.96 AS.seek 0.769ml @16:13:39.07 Vacuum too high -- waiting up to 40s @16:13:40.18 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:13:40.20 SS.reconfigure SSinhale.with maxSpeed: 39 @16:13:40.25 SS.seek 25ml @16:13:40.76 Stroke completed -- waiting for pressure to equalize ... @16:13:43.37 Intake.select :close @16:13:43.54 SSV.dial :exhaust @16:13:44.32 Exhaust.select :open @16:13:44.43 SS.reconfigure SSexhale @16:13:44.48 SS.seek 0ml @16:13:53.19 Exhaust.select :close @16:13:53.31 SSV.dial CBV @16:13:53.34 CBV.dial SSV @16:13:54.15 Allowing 130s for 25.0ml of remaining 1050.0ml @16:13:54.16 Intake.select :open @16:13:56.35 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:13:56.37 SS.reconfigure SSinhale.with maxSpeed: 39 @16:13:56.42 SS.seek 25ml @16:14:42.12 Stroke completed -- waiting for pressure to equalize ... @16:14:46.00 Intake.select :close @16:14:46.16 SSV.dial :exhaust @16:14:46.95 Exhaust.select :open @16:14:47.06 SS.reconfigure SSexhale @16:14:47.11 SS.seek 0ml @16:14:56.06 Exhaust.select :close @16:14:56.18 SSV.dial CBV @16:14:56.21 CBV.dial SSV @16:14:57.01 Allowing 130s for 25.0ml of remaining 1025.0ml @16:14:57.02 Intake.select :open @16:14:59.20 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:14:59.22 SS.reconfigure SSinhale.with maxSpeed: 39 @16:14:59.27 SS.seek 25ml @16:15:44.72 Stroke completed -- waiting for pressure to equalize ... @16:15:49.18 Intake.select :close @16:15:49.34 SSV.dial :exhaust @16:15:50.10 Exhaust.select :open @16:15:50.22 SS.reconfigure SSexhale @16:15:50.28 SS.seek 0ml @16:15:57.00 delay 3 seconds @16:15:59.47 Exhaust.select :close @16:15:59.59 SSV.dial CBV @16:15:59.62 CBV.dial SSV @16:16:00.01 ATV.dial :air3 @16:16:00.41 Allowing 130s for 25.0ml of remaining 1000.0ml @16:16:00.42 Intake.select :open @16:16:00.55 delay 3 seconds @16:16:02.65 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:16:02.67 SS.reconfigure SSinhale.with maxSpeed: 39 @16:16:02.72 SS.seek 25ml @16:16:03.64 AS.seek 0.794ml @16:16:08.94 delay 3 seconds @16:16:11.96 ATV.dial :bleach @16:16:12.56 AS.seek 0.779ml @16:16:15.85 delay 3 seconds @16:16:18.87 ATV.dial :AIV @16:16:20.04 AS.reconfigure ASconfig @16:16:20.09 AS.seek 0.015ml @16:16:47.92 Stroke completed -- waiting for pressure to equalize ... @16:16:51.79 Intake.select :close @16:16:51.95 SSV.dial :exhaust @16:16:52.73 Exhaust.select :open @16:16:52.84 SS.reconfigure SSexhale @16:16:52.90 SS.seek 0ml @16:17:01.58 Exhaust.select :close @16:17:01.70 SSV.dial CBV @16:17:01.76 CBV.dial SSV @16:17:02.52 Allowing 130s for 25.0ml of remaining 975.0ml @16:17:02.53 Intake.select :open @16:17:04.31 ATV.dial :waste @16:17:04.72 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:17:04.74 SS.reconfigure SSinhale.with maxSpeed: 39 @16:17:04.79 SS.seek 25ml @16:17:05.69 AS.seek :empty @16:17:06.91 ATV.dial :air3 @16:17:07.56 AS.reconfigure ASslow1 @16:17:07.61 AS.seek 0.009ml @16:17:09.97 delay 2 seconds @16:17:11.98 ATV.dial :bleach @16:17:12.43 AS.seek 0.579ml @16:17:50.58 Stroke completed -- waiting for pressure to equalize ... @16:17:55.12 Intake.select :close @16:17:55.28 SSV.dial :exhaust @16:17:56.05 Exhaust.select :open @16:17:56.16 SS.reconfigure SSexhale @16:17:56.21 SS.seek 0ml @16:18:05.50 Exhaust.select :close @16:18:05.62 SSV.dial CBV @16:18:05.65 CBV.dial SSV @16:18:06.46 Allowing 130s for 25.0ml of remaining 950.0ml @16:18:06.47 Intake.select :open @16:18:08.66 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:18:08.68 SS.reconfigure SSinhale.with maxSpeed: 39 @16:18:08.72 SS.seek 25ml @16:18:54.15 Stroke completed -- waiting for pressure to equalize ... @16:18:58.61 Intake.select :close @16:18:58.77 SSV.dial :exhaust @16:18:59.58 Exhaust.select :open @16:18:59.74 SS.reconfigure SSexhale @16:18:59.79 SS.seek 0ml @16:19:07.30 delay 3 seconds @16:19:08.86 Exhaust.select :close @16:19:08.98 SSV.dial CBV @16:19:09.01 CBV.dial SSV @16:19:09.80 Allowing 130s for 25.0ml of remaining 925.0ml @16:19:09.81 Intake.select :open @16:19:10.31 ATV.dial :air3 @16:19:10.85 delay 3 seconds @16:19:12.00 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:19:12.02 SS.reconfigure SSinhale.with maxSpeed: 39 @16:19:12.07 SS.seek 25ml @16:19:13.94 AS.seek 0.604ml @16:19:19.25 delay 3 seconds @16:19:22.27 ATV.dial :bleach @16:19:22.87 AS.seek 0.589ml @16:19:26.13 delay 3 seconds @16:19:29.15 ATV.dial :ASV @16:19:30.11 ASV.dial :wasteR @16:19:30.68 AS.seek 0ml @16:19:47.21 BACfans.email "Started mfb in tube 2 at 14:05:14CEST03-Oct-18\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @16:20:27.32 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @16:20:27.35 Stroke completed -- waiting for pressure to equalize ... @16:20:27.38 Retry #4 of 10 after 10:00 @16:20:28.06 Intake.select :close @16:20:28.23 SSV.dial :exhaust @16:20:29.01 Exhaust.select :open @16:20:29.12 SS.reconfigure SSexhale @16:20:29.17 SS.seek 0ml @16:20:38.17 Exhaust.select :close @16:20:38.29 SSV.dial CBV @16:20:38.32 CBV.dial SSV @16:20:39.13 Allowing 130s for 25.0ml of remaining 900.0ml @16:20:39.14 Intake.select :open @16:20:41.32 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:20:41.34 SS.reconfigure SSinhale.with maxSpeed: 39 @16:20:41.39 SS.seek 25ml @16:21:09.94 Vacuum too high -- waiting up to 40s @16:21:11.31 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:21:11.33 SS.reconfigure SSinhale.with maxSpeed: 35 @16:21:11.38 SS.seek 25ml @16:21:29.43 delay 1:00 @16:21:30.23 Stroke completed -- waiting for pressure to equalize ... @16:21:34.13 Intake.select :close @16:21:34.29 SSV.dial :exhaust @16:21:35.06 Exhaust.select :open @16:21:35.18 SS.reconfigure SSexhale @16:21:35.23 SS.seek 0ml @16:21:44.14 Exhaust.select :close @16:21:44.26 SSV.dial CBV @16:21:44.29 CBV.dial SSV @16:21:45.12 Allowing 130s for 25.0ml of remaining 875.0ml @16:21:45.15 Intake.select :open @16:21:48.43 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:21:48.47 SS.reconfigure SSinhale.with maxSpeed: 35 @16:21:48.53 SS.seek 25ml @16:22:29.45 AFV.select :carrierSolution @16:22:29.56 delay 2 seconds @16:22:31.59 AS.reconfigure ASconfig @16:22:31.64 AS.seek 1ml @16:22:39.22 Stroke completed -- waiting for pressure to equalize ... @16:22:43.76 Intake.select :close @16:22:43.93 SSV.dial :exhaust @16:22:44.72 Exhaust.select :open @16:22:44.83 SS.reconfigure SSexhale @16:22:44.89 SS.seek 0ml @16:22:53.94 Exhaust.select :close @16:22:54.06 SSV.dial CBV @16:22:54.09 CBV.dial SSV @16:22:54.90 Allowing 130s for 25.0ml of remaining 850.0ml @16:22:54.91 Intake.select :open @16:22:57.10 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:22:57.12 SS.reconfigure SSinhale.with maxSpeed: 35 @16:22:57.17 SS.seek 25ml @16:23:29.38 delay 1 second @16:23:30.39 AFV.select :holdingCoil @16:23:30.50 delay 1 second @16:23:31.52 ATV.dial ASV @16:23:31.54 ASV.dial :wasteR @16:23:31.56 AS.reconfigure ASslow1 @16:23:31.60 AS.seek 0ml @16:23:47.59 Stroke completed -- waiting for pressure to equalize ... @16:23:52.12 Intake.select :close @16:23:52.28 SSV.dial :exhaust @16:23:53.05 Exhaust.select :open @16:23:53.16 SS.reconfigure SSexhale @16:23:53.21 SS.seek 0ml @16:24:02.14 Exhaust.select :close @16:24:02.26 SSV.dial CBV @16:24:02.29 CBV.dial SSV @16:24:03.10 Allowing 130s for 25.0ml of remaining 825.0ml @16:24:03.11 Intake.select :open @16:24:05.29 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:24:05.31 SS.reconfigure SSinhale.with maxSpeed: 35 @16:24:05.36 SS.seek 25ml @16:24:55.86 Stroke completed -- waiting for pressure to equalize ... @16:25:01.00 Intake.select :close @16:25:01.16 SSV.dial :exhaust @16:25:01.92 Exhaust.select :open @16:25:02.04 SS.reconfigure SSexhale @16:25:02.10 SS.seek 0ml @16:25:10.90 Exhaust.select :close @16:25:11.02 SSV.dial CBV @16:25:11.05 CBV.dial SSV @16:25:11.87 Allowing 130s for 25.0ml of remaining 800.0ml @16:25:11.88 Intake.select :open @16:25:14.06 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:25:14.59 SS.reconfigure SSinhale.with maxSpeed: 35 @16:25:14.72 SS.seek 25ml @16:26:05.47 Stroke completed -- waiting for pressure to equalize ... @16:26:10.65 Intake.select :close @16:26:10.81 SSV.dial :exhaust @16:26:11.59 Exhaust.select :open @16:26:11.70 SS.reconfigure SSexhale @16:26:11.76 SS.seek 0ml @16:26:20.56 Exhaust.select :close @16:26:20.68 SSV.dial CBV @16:26:20.71 CBV.dial SSV @16:26:21.51 Allowing 130s for 25.0ml of remaining 775.0ml @16:26:21.52 Intake.select :open @16:26:23.71 Intake @35.0psia, vacuum from 20.0 to 24.0psi @16:26:23.73 SS.reconfigure SSinhale.with maxSpeed: 35 @16:26:23.78 SS.seek 25ml @16:26:48.91 Vacuum too high -- waiting up to 40s @16:26:50.02 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:26:50.04 SS.reconfigure SSinhale.with maxSpeed: 32 @16:26:50.09 SS.seek 25ml @16:26:53.02 delay 20 seconds @16:27:13.04 ATV.dial :air3 @16:27:14.45 AS.seek 0.59ml @16:27:18.24 Stroke completed -- waiting for pressure to equalize ... @16:27:23.42 Intake.select :close @16:27:23.58 SSV.dial :exhaust @16:27:24.36 Exhaust.select :open @16:27:24.48 SS.reconfigure SSexhale @16:27:24.53 SS.seek 0ml @16:27:33.65 Exhaust.select :close @16:27:33.77 SSV.dial CBV @16:27:33.83 CBV.dial SSV @16:27:34.59 Allowing 130s for 25.0ml of remaining 750.0ml @16:27:34.60 Intake.select :open @16:27:36.79 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:27:36.81 SS.reconfigure SSinhale.with maxSpeed: 32 @16:27:36.85 SS.seek 25ml @16:28:31.81 Stroke completed -- waiting for pressure to equalize ... @16:28:37.59 Intake.select :close @16:28:37.75 SSV.dial :exhaust @16:28:39.13 Exhaust.select :open @16:28:39.24 SS.reconfigure SSexhale @16:28:39.30 SS.seek 0ml @16:28:48.50 Exhaust.select :close @16:28:48.62 SSV.dial CBV @16:28:48.65 CBV.dial SSV @16:28:49.46 Allowing 130s for 25.0ml of remaining 725.0ml @16:28:49.47 Intake.select :open @16:28:51.65 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:28:51.67 SS.reconfigure SSinhale.with maxSpeed: 32 @16:28:51.72 SS.seek 25ml @16:29:13.40 delay 3 seconds @16:29:16.42 ATV.dial :ASV @16:29:17.73 ASV.dial :wasteR @16:29:17.81 AS.seek 0.09ml @16:29:46.54 Stroke completed -- waiting for pressure to equalize ... @16:29:52.36 Intake.select :close @16:29:52.52 SSV.dial :exhaust @16:29:53.30 Exhaust.select :open @16:29:53.41 SS.reconfigure SSexhale @16:29:53.46 SS.seek 0ml @16:30:02.57 Exhaust.select :close @16:30:02.69 SSV.dial CBV @16:30:02.72 CBV.dial SSV @16:30:03.52 Allowing 130s for 25.0ml of remaining 700.0ml @16:30:03.53 Intake.select :open @16:30:05.71 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:30:05.73 SS.reconfigure SSinhale.with maxSpeed: 32 @16:30:05.78 SS.seek 25ml @16:30:27.41 BACfans.email "Started mfb in tube 2 at 14:05:14CEST03-Oct-18\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @16:31:07.57 delay 30 seconds @16:31:07.60 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @16:31:07.62 Vacuum too high -- waiting up to 40s @16:31:07.64 Retry #5 of 10 after 10:00 @16:31:07.97 Intake @35.1psia, vacuum from 20.0 to 24.0psi @16:31:08.00 SS.reconfigure SSinhale.with maxSpeed: 29 @16:31:08.04 SS.seek 25ml @16:31:37.61 ATV.dial :extSrc @16:31:38.00 Stroke completed -- waiting for pressure to equalize ... @16:31:38.35 delay 3 seconds @16:31:41.36 ATV.dial :ElutionWater @16:31:42.51 AS.seek 0.12ml @16:31:42.65 Intake.select :close @16:31:42.81 SSV.dial :exhaust @16:31:43.58 Exhaust.select :open @16:31:43.69 SS.reconfigure SSexhale @16:31:43.74 SS.seek 0ml @16:31:48.83 ATV.dial :air3 @16:31:49.44 AS.seek 0.13ml @16:31:51.71 delay 2 seconds @16:31:52.78 Exhaust.select :close @16:31:52.90 SSV.dial CBV @16:31:52.93 CBV.dial SSV @16:31:53.73 Allowing 130s for 25.0ml of remaining 675.0ml @16:31:53.74 Intake.select :open @16:31:53.79 ATV.dial :bleach @16:31:54.25 AS.seek 0.23ml @16:31:56.50 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:31:56.52 SS.reconfigure SSinhale.with maxSpeed: 29 @16:31:56.57 SS.seek 25ml @16:32:14.65 delay 3 seconds @16:32:17.67 ATV.dial :air3 @16:32:18.20 delay 3 seconds @16:32:21.29 AS.seek 0.255ml @16:32:26.63 delay 3 seconds @16:32:29.65 ATV.dial :bleach @16:32:30.25 AS.seek 0.24ml @16:32:33.57 delay 3 seconds @16:32:36.59 ATV.dial :ASV @16:32:37.56 ASV.dial :SPEdelivery @16:32:38.01 AFV.select :carrierSolution @16:32:38.12 delay 2 seconds @16:32:40.15 AS.reconfigure ASconfig @16:32:40.20 AS.seek 0.5ml @16:32:55.45 delay 1 second @16:32:56.46 AFV.select :holdingCoil @16:32:56.62 AS.reconfigure ASslow1 @16:32:56.67 AS.seek 0.17ml @16:32:57.14 Stroke completed -- waiting for pressure to equalize ... @16:33:02.92 Intake.select :close @16:33:03.09 SSV.dial :exhaust @16:33:03.87 Exhaust.select :open @16:33:03.98 SS.reconfigure SSexhale @16:33:04.03 SS.seek 0ml @16:33:12.84 Exhaust.select :close @16:33:12.96 SSV.dial CBV @16:33:12.99 CBV.dial SSV @16:33:13.80 Allowing 130s for 25.0ml of remaining 650.0ml @16:33:13.81 Intake.select :open @16:33:16.00 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:33:16.02 SS.reconfigure SSinhale.with maxSpeed: 29 @16:33:16.06 SS.seek 25ml @16:34:03.32 SPE.hold 2:46:40,95C @16:34:03.39 delay 3:00 @16:34:16.95 Stroke completed -- waiting for pressure to equalize ... @16:34:23.41 Intake.select :close @16:34:23.57 SSV.dial :exhaust @16:34:24.37 Exhaust.select :open @16:34:24.48 SS.reconfigure SSexhale @16:34:24.53 SS.seek 0ml @16:34:33.67 Exhaust.select :close @16:34:33.79 SSV.dial CBV @16:34:33.82 CBV.dial SSV @16:34:34.65 Allowing 130s for 25.0ml of remaining 625.0ml @16:34:34.66 Intake.select :open @16:34:36.84 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:34:36.86 SS.reconfigure SSinhale.with maxSpeed: 29 @16:34:36.91 SS.seek 25ml @16:35:36.00 Vacuum too high -- waiting up to 40s @16:35:37.98 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:35:38.00 SS.reconfigure SSinhale.with maxSpeed: 26 @16:35:38.05 SS.seek 25ml @16:35:39.82 Stroke completed -- waiting for pressure to equalize ... @16:35:44.34 Intake.select :close @16:35:44.50 SSV.dial :exhaust @16:35:45.29 Exhaust.select :open @16:35:45.41 SS.reconfigure SSexhale @16:35:45.46 SS.seek 0ml @16:35:54.67 Exhaust.select :close @16:35:54.79 SSV.dial CBV @16:35:54.82 CBV.dial SSV @16:35:55.65 Allowing 130s for 25.0ml of remaining 600.0ml @16:35:55.66 Intake.select :open @16:35:57.84 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:35:57.86 SS.reconfigure SSinhale.with maxSpeed: 26 @16:35:57.91 SS.seek 25ml @16:37:03.42 AFV.select :carrierSolution @16:37:03.53 delay 2 seconds @16:37:05.34 Stroke completed -- waiting for pressure to equalize ... @16:37:05.55 AS.reconfigure ASconfig @16:37:05.60 AS.seek 1ml @16:37:11.15 Intake.select :close @16:37:11.32 SSV.dial :exhaust @16:37:12.12 Exhaust.select :open @16:37:12.23 SS.reconfigure SSexhale @16:37:12.28 SS.seek 0ml @16:37:21.26 Exhaust.select :close @16:37:21.38 SSV.dial CBV @16:37:21.41 CBV.dial SSV @16:37:22.23 Allowing 130s for 25.0ml of remaining 575.0ml @16:37:22.24 Intake.select :open @16:37:24.43 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:37:24.44 SS.reconfigure SSinhale.with maxSpeed: 26 @16:37:24.49 SS.seek 25ml @16:37:53.59 delay 1 second @16:37:54.60 AFV.select :holdingCoil @16:37:54.71 delay 1 second @16:37:55.72 ASV.dial :wasteD @16:37:56.65 AS.seek 0ml @16:38:32.28 Stroke completed -- waiting for pressure to equalize ... @16:38:39.35 Intake.select :close @16:38:39.51 SSV.dial :exhaust @16:38:40.90 Exhaust.select :open @16:38:41.01 SS.reconfigure SSexhale @16:38:41.06 SS.seek 0ml @16:38:50.04 Exhaust.select :close @16:38:50.16 SSV.dial CBV @16:38:50.19 CBV.dial SSV @16:38:50.99 Allowing 130s for 25.0ml of remaining 550.0ml @16:38:51.00 Intake.select :open @16:38:53.19 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:38:53.21 SS.reconfigure SSinhale.with maxSpeed: 26 @16:38:53.26 SS.seek 25ml @16:38:54.45 Priming Eluate @16:38:54.46 AFV.select :carrierSolution @16:38:54.57 delay 2 seconds @16:38:56.59 AS.seek 0.5ml @16:39:25.65 delay 1 second @16:39:26.66 AFV.select :holdingCoil @16:39:26.77 ATV.dial ASV @16:39:26.78 ASV.dial :SPErecovery @16:39:27.34 Swish #1 of 2 @16:39:27.42 AS.reconfigure ASslow0 @16:39:27.47 AS.seek 0.6ml @16:39:37.95 AS.seek 0.5ml @16:39:48.36 Swish #2 of 2 @16:39:48.45 AS.seek 0.6ml @16:39:58.94 AS.seek 0.5ml @16:40:00.96 Stroke completed -- waiting for pressure to equalize ... @16:40:08.67 Intake.select :close @16:40:08.83 SSV.dial :exhaust @16:40:09.32 delay 3 seconds @16:40:09.62 Exhaust.select :open @16:40:09.73 SS.reconfigure SSexhale @16:40:09.78 SS.seek 0ml @16:40:12.41 AS.reconfigure ASslow1 @16:40:12.46 AS.seek 0.684ml @16:40:19.04 Exhaust.select :close @16:40:19.16 SSV.dial CBV @16:40:19.19 CBV.dial SSV @16:40:19.97 Allowing 130s for 25.0ml of remaining 525.0ml @16:40:19.98 Intake.select :open @16:40:22.16 Intake @35.0psia, vacuum from 20.0 to 24.0psi @16:40:22.18 SS.reconfigure SSinhale.with maxSpeed: 26 @16:40:22.23 SS.seek 25ml @16:40:49.76 delay 3 seconds @16:40:52.79 Eluate primed @16:40:52.80 ATV.dial :waste2 @16:40:53.46 AS.reconfigure ASnorm @16:40:53.51 AS.seek 0ml @16:41:02.16 Vacuum too high -- waiting up to 40s @16:41:03.79 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:41:03.81 SS.reconfigure SSinhale.with maxSpeed: 23 @16:41:03.86 SS.seek 25ml @16:41:07.67 BACfans.email "Started mfb in tube 2 at 14:05:14CEST03-Oct-18\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @16:41:47.79 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @16:41:47.83 Retry #6 of 10 after 10:00 @16:41:47.87 SPE.coast @16:41:47.89 Stroke completed -- waiting for pressure to equalize ... @16:41:47.98 ASV.dial :SPErecovery @16:41:48.00 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0316h1225ml.pcr @16:41:48.02 Building PCR Slug from ASV with MM2 mix, PP4 primer @16:41:48.03 Priming with carrier @16:41:48.04 ATV.dial ARV @16:41:49.25 ARV.dial :carrier @16:41:49.38 Intake.select :close @16:41:49.55 SSV.dial :exhaust @16:41:49.70 AS.seek 0.5ml @16:41:50.33 Exhaust.select :open @16:41:50.45 SS.reconfigure SSexhale @16:41:50.51 SS.seek 0ml @16:41:59.57 Exhaust.select :close @16:41:59.69 SSV.dial CBV @16:41:59.72 CBV.dial SSV @16:42:00.52 Allowing 130s for 25.0ml of remaining 500.0ml @16:42:00.53 Intake.select :open @16:42:02.71 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:42:02.73 SS.reconfigure SSinhale.with maxSpeed: 23 @16:42:02.78 SS.seek 25ml @16:42:15.25 AFV.select :carrierSolution @16:42:15.37 delay 2 seconds @16:42:17.40 AS.reconfigure ASconfig @16:42:17.45 AS.seek 1ml @16:42:46.53 delay 1 second @16:42:47.54 AFV.select :holdingCoil @16:42:47.65 delay 1 second @16:42:48.67 ATV.dial :waste @16:42:50.06 AS.reconfigure ASnorm @16:42:50.11 AS.seek :empty @16:43:18.89 Stroke completed -- waiting for pressure to equalize ... @16:43:26.00 Intake.select :close @16:43:26.17 SSV.dial :exhaust @16:43:26.94 Exhaust.select :open @16:43:27.05 SS.reconfigure SSexhale @16:43:27.11 SS.seek 0ml @16:43:36.02 Exhaust.select :close @16:43:36.13 SSV.dial CBV @16:43:36.16 CBV.dial SSV @16:43:36.97 Allowing 130s for 25.0ml of remaining 475.0ml @16:43:36.98 Intake.select :open @16:43:39.16 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:43:39.18 SS.reconfigure SSinhale.with maxSpeed: 23 @16:43:39.23 SS.seek 25ml @16:43:40.83 AFV.select :carrierSolution @16:43:40.95 delay 2 seconds @16:43:42.98 AS.reconfigure ASconfig @16:43:43.03 AS.seek 0.5ml @16:44:12.12 delay 1 second @16:44:13.13 AFV.select :holdingCoil @16:44:13.24 Building Slug @16:44:13.25 ATV.dial :ARV @16:44:14.75 delay 2 seconds @16:44:16.77 ARV.dial :air1 @16:44:17.18 delay 2 seconds @16:44:19.26 AS.reconfigure ASslow2 @16:44:19.32 AS.seek 0.506ml @16:44:25.51 delay 2 seconds @16:44:27.53 ARV.dial :MM2 @16:44:28.92 delay 2 seconds @16:44:30.96 Adding MM2 @16:44:31.03 AS.seek 0.524ml @16:44:49.39 delay 25 seconds @16:44:55.68 Stroke completed -- waiting for pressure to equalize ... @16:45:03.38 Intake.select :close @16:45:03.54 SSV.dial :exhaust @16:45:04.32 Exhaust.select :open @16:45:04.43 SS.reconfigure SSexhale @16:45:04.48 SS.seek 0ml @16:45:13.35 Exhaust.select :close @16:45:13.47 SSV.dial CBV @16:45:13.50 CBV.dial SSV @16:45:14.30 Allowing 130s for 25.0ml of remaining 450.0ml @16:45:14.31 Intake.select :open @16:45:14.40 ARV.dial :PP4 @16:45:14.79 Adding PP4 @16:45:14.80 delay 2 seconds @16:45:16.53 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:45:16.55 SS.reconfigure SSinhale.with maxSpeed: 23 @16:45:16.60 SS.seek 25ml @16:45:16.89 AS.seek 0.53ml @16:45:23.20 delay 10 seconds @16:45:33.22 ARV.dial :air3 @16:45:33.59 adding ARV air @16:45:33.60 delay 2 seconds @16:45:35.69 AS.seek 0.536ml @16:45:42.00 delay 5 seconds @16:45:47.02 ARV.dial :carrier @16:45:48.21 delay 7 seconds @16:45:55.30 AS.seek 0.594ml @16:46:17.78 Vacuum too high -- waiting up to 40s @16:46:19.41 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:46:19.43 SS.reconfigure SSinhale.with maxSpeed: 21 @16:46:19.48 SS.seek 25ml @16:46:36.35 Stroke completed -- waiting for pressure to equalize ... @16:46:44.06 Intake.select :close @16:46:44.22 SSV.dial :exhaust @16:46:44.99 Exhaust.select :open @16:46:45.10 SS.reconfigure SSexhale @16:46:45.15 SS.seek 0ml @16:46:53.94 Exhaust.select :close @16:46:54.01 delay 5 seconds @16:46:54.06 SSV.dial CBV @16:46:54.09 CBV.dial SSV @16:46:54.90 Allowing 130s for 25.0ml of remaining 425.0ml @16:46:54.91 Intake.select :open @16:46:57.10 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:46:57.12 SS.reconfigure SSinhale.with maxSpeed: 21 @16:46:57.16 SS.seek 25ml @16:46:59.03 ATV.dial :ASV @16:46:59.40 delay 2 seconds @16:47:01.49 AS.seek 0.6ml @16:47:07.81 delay 4 seconds @16:47:11.83 ATV.dial ARV @16:47:12.98 delay 2 seconds @16:47:15.00 ARV.dial :carrier @16:47:15.02 delay 2 seconds @16:47:17.11 AS.seek 0.65ml @16:48:07.65 delay 4 seconds @16:48:11.67 ATV.dial PCR @16:48:12.25 ASV.dial :waste1 @16:48:12.98 ARV.dial :air1 @16:48:13.36 Positioning Slug by 0.324ml @16:48:13.45 AS.seek 0.326ml @16:48:20.54 Stroke completed -- waiting for pressure to equalize ... @16:48:28.86 Intake.select :close @16:48:29.03 SSV.dial :exhaust @16:48:29.80 Exhaust.select :open @16:48:29.91 SS.reconfigure SSexhale @16:48:29.97 SS.seek 0ml @16:48:39.37 Exhaust.select :close @16:48:39.49 SSV.dial CBV @16:48:39.52 CBV.dial SSV @16:48:40.32 Allowing 130s for 25.0ml of remaining 400.0ml @16:48:40.34 Intake.select :open @16:48:42.51 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:48:42.53 SS.reconfigure SSinhale.with maxSpeed: 21 @16:48:42.58 SS.seek 25ml @16:50:05.95 Stroke completed -- waiting for pressure to equalize ... @16:50:14.90 Intake.select :close @16:50:15.06 SSV.dial :exhaust @16:50:15.83 Exhaust.select :open @16:50:15.94 SS.reconfigure SSexhale @16:50:16.01 SS.seek 0ml @16:50:25.00 Exhaust.select :close @16:50:25.12 SSV.dial CBV @16:50:25.15 CBV.dial SSV @16:50:25.96 Allowing 130s for 25.0ml of remaining 375.0ml @16:50:25.97 Intake.select :open @16:50:28.15 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:50:28.17 SS.reconfigure SSinhale.with maxSpeed: 21 @16:50:28.22 SS.seek 25ml @16:51:34.69 Vacuum too high -- waiting up to 40s @16:51:36.58 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:51:36.60 SS.reconfigure SSinhale.with maxSpeed: 19 @16:51:36.65 SS.seek 25ml @16:51:47.88 BACfans.email "Started mfb in tube 2 at 14:05:14CEST03-Oct-18\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @16:52:27.99 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @16:52:28.02 Stroke completed -- waiting for pressure to equalize ... @16:52:28.05 Retry #7 of 10 after 10:00 @16:52:28.74 Intake.select :close @16:52:28.90 SSV.dial :exhaust @16:52:29.68 Exhaust.select :open @16:52:29.79 SS.reconfigure SSexhale @16:52:29.85 SS.seek 0ml @16:52:38.96 Exhaust.select :close @16:52:39.08 SSV.dial CBV @16:52:39.11 CBV.dial SSV @16:52:39.91 Allowing 130s for 25.0ml of remaining 350.0ml @16:52:39.92 Intake.select :open @16:52:42.10 Intake @35.0psia, vacuum from 20.0 to 24.0psi @16:52:42.12 SS.reconfigure SSinhale.with maxSpeed: 19 @16:52:42.17 SS.seek 25ml @16:53:39.56 delay 5 seconds @16:53:44.58 ATV.dial ARV @16:53:45.31 ASV.dial :PCRlock @16:53:45.68 delay 5 seconds @16:53:50.71 Running Tt1 Profile60C300 thermocycling @16:53:50.72 PCR.program Profile60C300 @16:53:50.86 Profile60C300 should complete in < 1:45:21 @16:53:50.87 PCR.run 2:16:41.25 @16:54:14.23 Stroke completed -- waiting for pressure to equalize ... @16:54:23.23 Intake.select :close @16:54:23.39 SSV.dial :exhaust @16:54:24.16 Exhaust.select :open @16:54:24.27 SS.reconfigure SSexhale @16:54:24.34 SS.seek 0ml @16:54:33.48 Exhaust.select :close @16:54:33.60 SSV.dial CBV @16:54:33.63 CBV.dial SSV @16:54:34.43 Allowing 130s for 25.0ml of remaining 325.0ml @16:54:34.44 Intake.select :open @16:54:36.62 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:54:36.64 SS.reconfigure SSinhale.with maxSpeed: 19 @16:54:36.69 SS.seek 25ml @16:56:08.71 Stroke completed -- waiting for pressure to equalize ... @16:56:18.92 Intake.select :close @16:56:19.09 SSV.dial :exhaust @16:56:19.86 Exhaust.select :open @16:56:19.97 SS.reconfigure SSexhale @16:56:20.03 SS.seek 0ml @16:56:29.39 Exhaust.select :close @16:56:29.51 SSV.dial CBV @16:56:29.54 CBV.dial SSV @16:56:30.35 Allowing 130s for 25.0ml of remaining 300.0ml @16:56:30.36 Intake.select :open @16:56:32.55 Intake @35.2psia, vacuum from 20.0 to 24.0psi @16:56:32.57 SS.reconfigure SSinhale.with maxSpeed: 19 @16:56:32.61 SS.seek 25ml @16:57:29.22 Vacuum too high -- waiting up to 40s @16:57:31.13 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:57:31.15 SS.reconfigure SSinhale.with maxSpeed: 17 @16:57:31.20 SS.seek 25ml @16:58:10.56 Stroke completed -- waiting for pressure to equalize ... @16:58:20.24 Intake.select :close @16:58:20.41 SSV.dial :exhaust @16:58:21.19 Exhaust.select :open @16:58:21.30 SS.reconfigure SSexhale @16:58:21.35 SS.seek 0ml @16:58:30.26 Exhaust.select :close @16:58:30.38 SSV.dial CBV @16:58:30.41 CBV.dial SSV @16:58:31.22 Allowing 130s for 25.0ml of remaining 275.0ml @16:58:31.23 Intake.select :open @16:58:33.42 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:58:33.44 SS.reconfigure SSinhale.with maxSpeed: 17 @16:58:33.49 SS.seek 25ml @17:00:16.71 Stroke completed -- waiting for pressure to equalize ... @17:00:27.62 Intake.select :close @17:00:27.79 SSV.dial :exhaust @17:00:28.57 Exhaust.select :open @17:00:28.71 SS.reconfigure SSexhale @17:00:28.76 SS.seek 0ml @17:00:38.03 Exhaust.select :close @17:00:38.15 SSV.dial CBV @17:00:38.18 CBV.dial SSV @17:00:38.99 Allowing 130s for 25.0ml of remaining 250.0ml @17:00:39.00 Intake.select :open @17:00:41.18 Intake @34.9psia, vacuum from 20.0 to 24.0psi @17:00:41.20 SS.reconfigure SSinhale.with maxSpeed: 17 @17:00:41.25 SS.seek 25ml @17:02:23.95 Stroke completed -- waiting for pressure to equalize ... @17:02:28.08 BACfans.email "Started mfb in tube 2 at 14:05:14CEST03-Oct-18\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @17:03:08.19 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @17:03:08.20 Retry #8 of 10 after 10:00 @17:03:08.36 SS.reconfigure SSconfig @17:03:08.43 SS.hold @17:03:08.56 Intake.select :close @17:03:08.73 ShallowSampler::Clogged in WCR -- progress too slow after 1275.0ml @17:03:08.74 SSV.dial :exhaust @17:03:09.52 Exhaust.select :open @17:03:09.63 SS.reconfigure SSexhale @17:03:10.41 SS.seek 0ml @17:03:19.34 Exhaust.select :close @17:03:19.46 Sampled 1275.0ml @17:03:19.48 Drying puck before fixing for 4:00 @17:03:19.49 CBV.dial :waste2b @17:03:20.28 delay 1:00 @17:04:20.30 CSV.dial :air @17:04:20.76 CS.reconfigure CSfast @17:04:20.82 CS.seek 10ml @17:04:43.90 Pumping wcr puck from tube 2 up to 55psi... @17:04:43.91 CBV.dial SSV @17:04:45.25 CBV.dial between CSV and waste2b @17:04:46.22 CSV.dial between air and CTV @17:04:46.57 CS.seek 6.927ml @17:04:54.34 CTV.dial CSV,avoiding: :air @17:04:54.42 CSV.dial CTV @17:04:55.14 CS.reconfigure CSpressurize @17:04:55.20 CS.seek 0ml @17:05:13.55 CSV.dial :air @17:05:14.03 CS.reconfigure CSfast @17:05:14.09 CS.seek 10ml @17:05:36.87 CSV.dial between air and CTV @17:05:37.21 CS.seek 2.51ml @17:05:55.03 CTV.dial CSV,avoiding: :air @17:05:55.06 CSV.dial CTV @17:05:55.37 CS.reconfigure CSpressurize @17:05:55.43 CS.seek 0ml @17:06:00.02 CBV.dial :waste2b @17:06:00.39 CS.reconfigure CSconfig @17:06:00.44 CS.seek :empty @17:06:03.84 delay 1:00 @17:07:03.86 CTV.dial :air,avoiding: :intake @17:07:05.16 Fix #1 of 2 (with rnal) for 2:00 @17:07:05.18 CSV.dial CSR @17:07:05.69 Pulling 2ml of CSR.rnal @17:07:05.70 CSR.select :rnal @17:07:05.83 CS.seek 2ml @17:07:12.09 delay 5 seconds @17:07:17.11 CSR.select :air @17:07:17.24 CS.reconfigure CSfast @17:07:17.29 CS.seek 10ml @17:07:35.69 Pumping wcr puck from tube 2 up to 55psi... @17:07:35.70 CBV.dial SSV @17:07:37.01 CBV.dial between CSV and waste2b @17:07:38.62 CSV.dial between air and CTV @17:07:39.24 CS.seek 6.82ml @17:07:47.32 CTV.dial CSV,avoiding: :air @17:07:47.34 CSV.dial CTV @17:07:48.72 CS.reconfigure CSpressurize @17:07:48.78 CS.seek 0ml @17:08:06.90 CSV.dial :air @17:08:07.37 CS.reconfigure CSfast @17:08:07.43 CS.seek 10ml @17:08:30.25 CSV.dial between air and CTV @17:08:30.64 CS.seek 1.989ml @17:08:49.16 CTV.dial CSV,avoiding: :air @17:08:49.19 CSV.dial CTV @17:08:49.50 CS.reconfigure CSpressurize @17:08:49.56 CS.seek 0ml @17:08:50.68 delay 2:00 @17:10:50.70 CBV.dial :waste2b @17:10:51.05 CS.reconfigure CSconfig @17:10:51.10 CS.seek :empty @17:10:56.81 delay 1:00 @17:11:56.84 CTV.dial :air,avoiding: :intake @17:11:58.14 Fix #2 of 2 (with rnal) for 2:00 @17:11:58.16 CSV.dial CSR @17:11:58.67 Pulling 2ml of CSR.rnal @17:11:58.69 CSR.select :rnal @17:11:58.81 CS.seek 2ml @17:12:04.88 delay 5 seconds @17:12:09.90 CSR.select :air @17:12:10.03 CS.reconfigure CSfast @17:12:10.08 CS.seek 10ml @17:12:28.53 Pumping wcr puck from tube 2 up to 55psi... @17:12:28.54 CBV.dial SSV @17:12:29.85 CBV.dial between CSV and waste2b @17:12:30.84 CSV.dial between air and CTV @17:12:31.47 CS.seek 6.82ml @17:12:39.53 CTV.dial CSV,avoiding: :air @17:12:39.56 CSV.dial CTV @17:12:41.60 CS.reconfigure CSpressurize @17:12:41.66 CS.seek 0ml @17:12:59.75 CSV.dial :air @17:13:00.22 CS.reconfigure CSfast @17:13:00.28 CS.seek 10ml @17:13:08.23 BACfans.email "Started mfb in tube 2 at 14:05:14CEST03-Oct-18\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @17:13:48.37 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @17:13:48.44 Retry #9 of 10 after 10:00 @17:13:48.50 CSV.dial between air and CTV @17:13:48.84 CS.seek 2.056ml @17:14:07.29 CTV.dial CSV,avoiding: :air @17:14:07.32 CSV.dial CTV @17:14:07.64 CS.reconfigure CSpressurize @17:14:07.70 CS.seek 0ml @17:14:09.12 delay 2:00 @17:16:09.14 CBV.dial :waste2b @17:16:09.51 CS.reconfigure CSconfig @17:16:09.56 CS.seek :empty @17:16:14.89 delay 1:00 @17:17:14.92 CTV.dial :air,avoiding: :intake @17:17:16.33 Storing wcr puck in Collection Clamp to tube 1 @17:17:16.53 SC.reconfigure SCtight @17:17:16.58 SC.seek 1 @17:17:18.74 SC.reconfigure SCloose @17:17:18.97 Elbow.reconfigure TightElbow @17:17:19.02 Elbow.seek 1 @17:17:21.00 Forearm.reconfigure TightForearm @17:17:21.06 Forearm.seek 1 + 700 counts @17:17:22.11 SE.reconfigure SEpick @17:17:22.18 SE.seek beamBreak @17:17:24.24 SE.configure SEconfig @17:17:24.41 Elbow.reconfigure LimpElbow @17:17:24.46 Forearm.reconfigure LooseForearm @17:17:24.51 Forearm.seek 1 @17:17:24.80 Elbow.hold @17:17:24.88 Elbow.reconfigure TightElbow @17:17:24.99 SE.seek 10.09 Pucks below Top Plate @17:17:25.50 Forearm.force -20 @17:17:25.55 Forearm.seek 1 + 337 counts @17:17:26.51 Forearm.reconfigure TightForearm @17:17:26.57 Forearm.seek 1 + 700 counts @17:17:26.91 SE.seek 11.09 Pucks below Top Plate @17:17:27.64 Forearm.reconfigure LooseForearm @17:17:27.69 Forearm.seek :retract @17:17:28.60 Elbow.seek CC @17:17:30.33 Elbow.reconfigure LooseElbow @17:17:30.44 Forearm.seek CC @17:17:31.43 Hand.close @17:17:32.13 CC.seek :open @17:17:36.66 Forearm.seek :retract @17:17:37.63 Elbow.reconfigure TightElbow @17:17:37.68 Elbow.seek 1 @17:17:39.62 Elbow.reconfigure LimpElbow @17:17:39.68 Forearm.seek 1 @17:17:40.69 Elbow.hold @17:17:40.77 Elbow.reconfigure TightElbow @17:17:40.82 Hand.open @17:17:41.65 Forearm.force -20 @17:17:41.70 Forearm.seek 1 + 345 counts @17:17:42.02 Forearm.reconfigure TightForearm @17:17:42.08 Forearm.seek 1 + 700 counts @17:17:42.40 SE.seek :down @17:17:43.57 Forearm.reconfigure LooseForearm @17:17:43.62 Forearm.seek :retract @17:17:44.06 SE.coast @17:17:44.57 SC.coast @17:17:44.61 Starting finalPurge @17:17:44.63 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:17:44.71 Hand.open @17:17:45.15 Elbow.seek FlushPuck::Garage @17:17:47.19 Elbow.reconfigure LooseElbow @17:17:47.30 Forearm.seek :garage @17:17:48.35 Hand.close @17:17:49.14 Forearm.seek :retract @17:17:50.15 Elbow.reconfigure TightElbow @17:17:50.20 Elbow.seek CC @17:17:53.13 Elbow.reconfigure LooseElbow @17:17:53.24 Forearm.reconfigure TightForearm @17:17:53.29 Forearm.seek CC @17:17:55.20 CC.seek :closed @17:17:59.47 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.84 @17:17:59.54 Hand.open @17:18:00.20 Forearm.reconfigure LooseForearm @17:18:00.25 Forearm.seek :retract @17:18:01.23 Flushing the Collection Valve::Manifold @17:18:01.37 CSV.dial CSR @17:18:01.88 Pulling 5ml of CSR.flush @17:18:01.89 CSR.select :flush @17:18:02.02 CS.seek 5ml @17:18:15.79 delay 5 seconds @17:18:20.81 CSR.select :air @17:18:20.99 CS.reconfigure CSfast @17:18:21.05 CS.seek 7.5ml @17:18:27.53 CSV.dial :waste @17:18:28.82 CS.reconfigure CSconfig @17:18:28.87 CS.seek 0ml @17:18:49.04 Flushing lower manifold's waste1 port @17:18:49.18 CSV.dial :flush @17:18:49.63 CS.reconfigure CSfast @17:18:49.68 CS.seek 5ml @17:19:01.57 delay 5 seconds @17:19:06.59 CSV.dial :air @17:19:07.11 CS.seek 7ml @17:19:12.63 CSV.dial CTV @17:19:12.66
CTV.dial CSV @17:19:14.03 CBV.dial :waste1 @17:19:14.83 CS.seek 0ml @17:19:31.01 Flushing lower manifold's waste2b port @17:19:31.15 CSV.dial :flush @17:19:31.91 CS.seek 5ml @17:19:43.76 delay 5 seconds @17:19:48.77 CSV.dial :air @17:19:49.31 CS.seek 7ml @17:19:55.20 CSV.dial CTV @17:19:55.23
CTV.dial CSV @17:19:55.68 CBV.dial :waste2b @17:19:56.46 CS.seek 0ml @17:20:12.79 Flushing upper manifold @17:20:12.93 CSV.dial :flush @17:20:13.69 CS.seek 5ml @17:20:25.67 delay 5 seconds @17:20:30.68 CSV.dial :air @17:20:31.24 CS.seek 7ml @17:20:36.73 CSV.dial CBV @17:20:36.76
CBV.dial CSV @17:20:38.45 CTV.dial :air @17:20:39.80 CS.seek 0ml @17:20:55.85 delay 7 seconds @17:21:02.87 CS.seek 3ml @17:21:10.50 CSV.dial :waste @17:21:10.97 CS.seek 0ml @17:21:18.60 CSV.dial :flush @17:21:19.07 CS.seek 3ml @17:21:26.73 delay 5 seconds @17:21:31.74 CSV.dial :air @17:21:32.28 CS.seek 5ml @17:21:37.69 CSV.dial :air @17:21:37.72 CS.seek 0ml @17:21:49.51 delay 7 seconds @17:21:56.53 CS.seek 3ml @17:22:04.09 CSV.dial :waste @17:22:04.85 CS.seek 0ml @17:22:12.70 Disinfecting external Intake with mfbkill @17:22:12.84 CSV.dial CSR @17:22:14.18 Pulling 8ml of CSR.mfbkill @17:22:14.19 CSR.select :mfbkill @17:22:14.32 CS.seek 8ml @17:22:32.61 delay 5 seconds @17:22:37.62 CSR.select :air @17:22:37.76 CS.seek 10ml @17:22:43.23 CSV.dial CBV @17:22:43.26
CBV.dial CSV @17:22:44.28 CTV.dial :intake @17:22:45.02 Intake.select :open @17:22:45.15 CS.seek 2ml @17:23:04.13 Intake.select :close @17:23:04.25 CTV.dial :air @17:23:05.01 CS.reconfigure CSconfig @17:23:05.06 CS.seek 0ml @17:23:11.14 Flushing lower manifold's waste1 port @17:23:11.15 CSV.dial CSR @17:23:12.23 Pulling 4ml of CSR.mfbkill @17:23:12.24 CSR.select :mfbkill @17:23:12.37 CS.seek 4ml @17:23:23.64 delay 5 seconds @17:23:28.66 CSR.select :air @17:23:28.79 CS.reconfigure CSfast @17:23:28.84 CS.seek 10ml @17:23:42.82 CSV.dial CTV @17:23:42.85
CTV.dial CSV @17:23:44.25 CBV.dial :waste1 @17:23:44.74 CS.seek 0ml @17:23:48.46 BACfans.email "Started mfb in tube 2 at 14:05:14CEST03-Oct-18\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @17:24:28.57 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @17:24:28.62
Flushing lower manifold's waste2b port @17:24:28.63 Retry #10 of 10 after 10:00 @17:24:28.65
CSV.dial CSR @17:24:29.18 Pulling 4ml of CSR.mfbkill @17:24:29.19 CSR.select :mfbkill @17:24:29.32 CS.reconfigure CSconfig @17:24:29.36 CS.seek 4ml @17:24:40.61 delay 5 seconds @17:24:45.63 CSR.select :air @17:24:45.75 CS.reconfigure CSfast @17:24:45.80 CS.seek 10ml @17:24:59.83 CSV.dial CTV @17:24:59.86
CTV.dial CSV @17:25:00.34 CBV.dial :waste2b @17:25:01.76 CS.seek 0ml @17:25:24.42 Killing upper manifold @17:25:24.43 CSV.dial CSR @17:25:24.95 Pulling 4ml of CSR.mfbkill @17:25:24.96 CSR.select :mfbkill @17:25:25.09 CS.reconfigure CSconfig @17:25:25.13 CS.seek 4ml @17:25:36.39 delay 5 seconds @17:25:41.41 CSR.select :air @17:25:41.54 CS.reconfigure CSfast @17:25:41.59 CS.seek 10ml @17:25:55.64 CSV.dial CBV @17:25:55.67
CBV.dial CSV @17:25:56.75 CTV.dial :air @17:25:58.06 CS.seek 0ml @17:26:20.73 delay 7 seconds @17:26:27.75 CS.seek 3ml @17:26:35.38 CSV.dial :waste @17:26:35.84 CS.seek 0ml @17:26:43.42 Flushing upper manifold @17:26:43.56 CSV.dial :flush @17:26:44.02 CS.seek 5ml @17:26:55.95 delay 5 seconds @17:27:00.97 CSV.dial :air @17:27:01.49 CS.seek 7ml @17:27:06.96 CSV.dial CBV @17:27:06.99
CBV.dial CSV @17:27:08.03 CTV.dial :air @17:27:08.05 CS.seek 0ml @17:27:24.14 delay 7 seconds @17:27:31.16 CS.seek 3ml @17:27:38.76 CSV.dial :waste @17:27:39.24 CS.seek 0ml @17:27:46.89 CSV.dial :flush @17:27:47.35 CS.seek 3ml @17:27:54.89 delay 5 seconds @17:27:59.90 CSV.dial :air @17:28:00.43 CS.seek 5ml @17:28:05.88 CSV.dial :air @17:28:05.91 CS.seek 0ml @17:28:17.67 delay 7 seconds @17:28:24.69 CS.seek 3ml @17:28:32.26 CSV.dial :waste @17:28:33.02 CS.seek 0ml @17:28:40.64 Flushing lower manifold's waste1 port @17:28:40.78 CSV.dial :flush @17:28:41.25 CS.seek 5ml @17:28:53.20 delay 5 seconds @17:28:58.22 CSV.dial :air @17:28:58.74 CS.seek 7.001ml @17:29:04.24 CSV.dial CTV @17:29:04.27
CTV.dial CSV @17:29:05.68 CBV.dial :waste1 @17:29:06.17 CS.seek 0ml @17:29:22.30 Flushing lower manifold's waste2b port @17:29:22.44 CSV.dial :flush @17:29:23.18 CS.seek 5ml @17:29:34.95 delay 5 seconds @17:29:39.96 CSV.dial :air @17:29:40.49 CS.seek 7ml @17:29:45.91 CSV.dial CTV @17:29:45.94
CTV.dial CSV @17:29:46.41 CBV.dial :waste2b @17:29:47.18 CS.seek 0ml @17:30:03.27 Disinfecting external Intake with kill @17:30:03.41 CSV.dial CSR @17:30:03.92 Pulling 8ml of CSR.kill @17:30:03.93 CSR.select :kill @17:30:04.06 CS.seek 8ml @17:30:22.33 delay 5 seconds @17:30:27.35 CSR.select :air @17:30:27.49 CS.seek 10ml @17:30:32.97 CSV.dial CBV @17:30:33.00
CBV.dial CSV @17:30:34.06 CTV.dial :intake @17:30:34.80 Intake.select :open @17:30:34.93 CS.seek 2ml @17:30:53.95 Intake.select :close @17:30:54.07 CTV.dial :air @17:30:54.81 CS.reconfigure CSconfig @17:30:54.86 CS.seek 0ml @17:31:00.95 CSV.dial CSR @17:31:02.03 Pulling 5ml of CSR.flush @17:31:02.04 CSR.select :flush @17:31:02.17 CS.seek 5ml @17:31:16.11 delay 5 seconds @17:31:21.13 CSR.select :air @17:31:21.27 CS.seek 10ml @17:31:35.04 CSV.dial CBV @17:31:36.05 CS.seek 0ml @17:32:02.74 Garaging FlushPuck in Collection Clamp @17:32:02.82 Elbow.reconfigure TightElbow @17:32:02.87 Elbow.seek CC @17:32:03.18 Elbow.reconfigure LooseElbow @17:32:03.28 Forearm.seek CC @17:32:04.24 Hand.close @17:32:04.90 CC.seek :open @17:32:09.24 Forearm.seek :retract @17:32:10.23 Elbow.reconfigure TightElbow @17:32:10.28 Elbow.seek :garage @17:32:13.43 Elbow.reconfigure LooseElbow @17:32:13.54 Forearm.reconfigure TightForearm @17:32:13.59 Forearm.seek :garage @17:32:14.77 Hand.open @17:32:15.47 Forearm.reconfigure LooseForearm @17:32:15.52 Forearm.seek :retract @17:32:16.51 Starting initialPurge @17:32:16.52 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:32:16.60 Hand.open @17:32:16.97 Elbow.reconfigure TightElbow @17:32:17.65 Elbow.seek FlushPuck::Garage @17:32:17.83 Elbow.reconfigure LooseElbow @17:32:17.94 Forearm.seek :garage @17:32:18.93 Hand.close @17:32:19.67 Forearm.seek :retract @17:32:20.67 Elbow.reconfigure TightElbow @17:32:20.72 Elbow.seek CC @17:32:23.56 Elbow.reconfigure LooseElbow @17:32:23.67 Forearm.reconfigure TightForearm @17:32:23.72 Forearm.seek CC @17:32:25.03 CC.seek :closed @17:32:29.34 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.86 @17:32:29.43 Hand.open @17:32:30.10 Forearm.reconfigure LooseForearm @17:32:30.15 Forearm.seek :retract @17:32:31.12 Flushing the Collection Valve::Manifold @17:32:31.25 CSV.dial CSR @17:32:32.33 Pulling 5ml of CSR.flush @17:32:32.34 CSR.select :flush @17:32:32.47 CS.seek 5ml @17:32:46.24 delay 5 seconds @17:32:51.25 CSR.select :air @17:32:51.42 CS.reconfigure CSfast @17:32:51.47 CS.seek 7.5ml @17:32:57.98 CSV.dial :waste @17:32:59.25 CS.reconfigure CSconfig @17:32:59.30 CS.seek 0ml @17:33:19.49 Purging external Intake @17:33:19.62 CSV.dial :flush @17:33:20.08 CS.reconfigure CSfast @17:33:20.13 CS.seek 10ml @17:33:42.58 delay 5 seconds @17:33:47.60 CSV.dial CBV @17:33:47.64
CBV.dial CSV @17:33:48.40 CTV.dial :intake @17:33:49.15 Intake.select :open @17:33:49.28 CS.seek 0ml @17:34:12.76 Intake.select :close @17:34:12.88 CTV.dial :air @17:34:13.63 Flushing upper manifold @17:34:13.76 CSV.dial :flush @17:34:14.50 CS.seek 5ml @17:34:26.37 delay 5 seconds @17:34:28.66 BACfans.email "Started mfb in tube 2 at 14:05:14CEST03-Oct-18\n{1 => 9, 2 => 13}\nCan@14:00:23, 14.1C, 68% humidity, 14.3psia, 14.355V, 0.651A, 0.651A avg, 9.34W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @17:35:08.77 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @17:35:08.78
CSV.dial :air @17:35:09.31 CS.seek 7ml @17:35:14.68 CSV.dial CBV @17:35:14.70
CBV.dial CSV @17:35:15.72 CTV.dial :air @17:35:15.74 CS.seek 0ml @17:35:31.87 delay 7 seconds @17:35:38.89 CS.seek 3ml @17:35:46.48 CSV.dial :waste @17:35:47.59 CS.seek 0ml @17:35:55.27 CSV.dial :flush @17:35:55.74 CS.seek 3ml @17:36:03.46 delay 5 seconds @17:36:08.47 CSV.dial :air @17:36:09.01 CS.seek 5ml @17:36:14.51 CSV.dial :air @17:36:14.54 CS.seek 0ml @17:36:26.49 delay 7 seconds @17:36:33.51 CS.seek 3ml @17:36:41.11 CSV.dial :waste @17:36:41.88 CS.seek 0ml @17:36:49.40 Garaging FlushPuck in Collection Clamp @17:36:49.48 Elbow.reconfigure TightElbow @17:36:49.53 Elbow.seek CC @17:36:49.80 Elbow.reconfigure LooseElbow @17:36:49.91 Forearm.seek CC @17:36:50.88 Hand.close @17:36:51.53 CC.seek :open @17:36:55.93 Forearm.seek :retract @17:36:56.89 Elbow.reconfigure TightElbow @17:36:56.94 Elbow.seek :garage @17:37:00.08 Elbow.reconfigure LooseElbow @17:37:00.19 Forearm.reconfigure TightForearm @17:37:00.24 Forearm.seek :garage @17:37:01.38 Hand.open @17:37:02.10 Forearm.reconfigure LooseForearm @17:37:02.15 Forearm.seek :retract @17:37:03.13 Completed [mbaPhase] begun 14:00:23 @17:37:03.15 Preparing to sleep until 14PM @17:37:03.19 deferred until MFBPCR finish @17:37:03.21
Waiting for MFBPCR til 14PM @17:37:03.24 Continuing at 14:00CEST04-Oct-18 @17:37:03.31 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/18tundeployv2.rb:115\n{1 => 12, 2 => 10}\nCan@17:37:03, 15.3C, 67% humidity, 14.4psia, 14.060V, 0.985A, 0.985A avg, 13.85W",Subject: "Waiting until 14:00CEST04-Oct-18" @17:37:43.42 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @17:37:43.43 Retry #1 of 10 after 10:00 @17:47:43.46 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/18tundeployv2.rb:115\n{1 => 12, 2 => 10}\nCan@17:37:03, 15.3C, 67% humidity, 14.4psia, 14.060V, 0.985A, 0.985A avg, 13.85W",Subject: "Waiting until 14:00CEST04-Oct-18" @17:48:23.57 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @17:48:23.58 Retry #2 of 10 after 10:00 @17:58:23.61 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/18tundeployv2.rb:115\n{1 => 12, 2 => 10}\nCan@17:37:03, 15.3C, 67% humidity, 14.4psia, 14.060V, 0.985A, 0.985A avg, 13.85W",Subject: "Waiting until 14:00CEST04-Oct-18" @17:59:03.72 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @17:59:03.73 Retry #3 of 10 after 10:00 @18:09:03.76 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/18tundeployv2.rb:115\n{1 => 12, 2 => 10}\nCan@17:37:03, 15.3C, 67% humidity, 14.4psia, 14.060V, 0.985A, 0.985A avg, 13.85W",Subject: "Waiting until 14:00CEST04-Oct-18" @18:09:43.87 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @18:09:43.88 Retry #4 of 10 after 10:00 @18:16:03.55 PCR.off @18:16:05.12 PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0316h1225ml.pcr @18:16:05.20 Cleaning PCR @18:16:05.21 AFV.select :carrierSolution @18:16:05.32 delay 2 seconds @18:16:07.35 AS.reconfigure ASconfig @18:16:07.40 AS.seek 0.5ml @18:16:17.69 delay 1 second @18:16:18.70 AFV.select :holdingCoil @18:16:18.81 ASV.dial :waste1 @18:16:19.19 ATV.dial :air3 @18:16:20.40 AS.reconfigure ASslow1 @18:16:20.45 AS.seek 0.51ml @18:16:22.74 delay 2 seconds @18:16:24.76 ATV.dial :bleach @18:16:25.35 AS.seek 0.81ml @18:17:25.99 delay 3 seconds @18:17:29.01 ATV.dial :air3 @18:17:29.55 delay 3 seconds @18:17:32.64 AS.seek 0.835ml @18:17:38.02 delay 3 seconds @18:17:41.04 ATV.dial :bleach @18:17:41.64 AS.seek 0.82ml @18:17:44.94 delay 3 seconds @18:17:47.96 ARV.dial :waste @18:17:48.55 delay 3 seconds @18:17:51.57 ATV.dial :ARV @18:17:52.51 AS.reconfigure ASconfig @18:17:52.56 AS.seek 0.47ml @18:18:12.96 AFV.select :carrierSolution @18:18:13.08 delay 2 seconds @18:18:15.11 AS.seek 1ml @18:18:45.89 delay 1 second @18:18:46.90 AFV.select :holdingCoil @18:18:47.01 delay 1 second @18:18:48.04 AS.reconfigure ASfast @18:18:48.09 AS.seek 0ml @18:19:15.42 AFV.select :carrierSolution @18:19:15.54 delay 2 seconds @18:19:17.57 AS.reconfigure ASconfig @18:19:17.62 AS.seek 1ml @18:19:43.91 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/18tundeployv2.rb:115\n{1 => 12, 2 => 10}\nCan@17:37:03, 15.3C, 67% humidity, 14.4psia, 14.060V, 0.985A, 0.985A avg, 13.85W",Subject: "Waiting until 14:00CEST04-Oct-18" @18:20:15.46 delay 1 second @18:20:16.48 AFV.select :holdingCoil @18:20:16.61 delay 1 second @18:20:17.64 AS.reconfigure ASfast @18:20:17.70 AS.seek 0ml @18:20:45.04 AFV.select :carrierSolution @18:20:45.16 delay 2 seconds @18:20:47.19 AS.reconfigure ASconfig @18:20:47.24 AS.seek 1ml @18:21:44.99 delay 1 second @18:21:46.00 AFV.select :holdingCoil @18:21:46.11 delay 1 second @18:21:47.14 AS.reconfigure ASfast @18:21:47.19 AS.seek 0ml @18:22:14.54 ARV.dial between carrier and air1 @18:22:15.02 ATV.dial :air3 @18:22:16.21 AS.reconfigure ASslow1 @18:22:16.26 AS.seek 0.01ml @18:22:18.58 delay 2 seconds @18:22:20.59 ATV.dial :bleach @18:22:21.20 AS.seek 0.81ml @18:25:02.36 delay 3 seconds @18:25:05.38 ATV.dial :air3 @18:25:05.91 delay 3 seconds @18:25:09.00 AS.seek 0.835ml @18:25:14.33 delay 3 seconds @18:25:17.35 ATV.dial :bleach @18:25:17.95 AS.seek 0.82ml @18:25:21.27 delay 3 seconds @18:25:24.29 ATV.dial PCR @18:25:24.68 AS.reconfigure ASconfig @18:25:24.73 AS.seek :empty @18:26:12.21 PCR.program :bleachProfile @18:26:12.27 PCR program should complete in < 2:45 @18:26:12.28 PCR.run 10:00 @18:29:20.16 PCR.off @18:29:20.19 AFV.select :carrierSolution @18:29:20.31 delay 2 seconds @18:29:22.34 AS.seek 1ml @18:30:20.16 delay 1 second @18:30:21.17 AFV.select :holdingCoil @18:30:21.28 delay 1 second @18:30:22.31 AS.reconfigure ASfast @18:30:22.36 AS.seek 0ml @18:30:49.71 delay 3 seconds @18:30:52.73 AFV.select :carrierSolution @18:30:52.84 delay 2 seconds @18:30:54.87 AS.reconfigure ASconfig @18:30:54.93 AS.seek 1ml @18:31:52.73 delay 1 second @18:31:53.74 AFV.select :holdingCoil @18:31:53.85 delay 1 second @18:31:54.88 AS.reconfigure ASfast @18:31:54.93 AS.seek 0ml @18:32:22.27 delay 3 seconds @18:32:25.29 AFV.select :carrierSolution @18:32:25.40 delay 2 seconds @18:32:27.43 AS.reconfigure ASconfig @18:32:27.49 AS.seek 1ml @18:33:25.29 delay 1 second @18:33:26.30 AFV.select :holdingCoil @18:33:26.41 delay 1 second @18:33:27.44 AS.reconfigure ASfast @18:33:27.49 AS.seek 0ml @18:33:54.84 delay 3 seconds @18:33:57.86 AFV.select :carrierSolution @18:33:57.97 delay 2 seconds @18:34:00.00 AS.reconfigure ASconfig @18:34:00.05 AS.seek 1ml @18:34:57.84 delay 1 second @18:34:58.85 AFV.select :holdingCoil @18:34:58.96 delay 1 second @18:34:59.99 AS.reconfigure ASfast @18:35:00.04 AS.seek 0ml @18:35:27.37 delay 3 seconds @18:35:30.39 AFV.select :carrierSolution @18:35:30.50 delay 2 seconds @18:35:32.53 AS.reconfigure ASconfig @18:35:32.59 AS.seek 1ml @18:36:30.43 delay 1 second @18:36:31.44 AFV.select :holdingCoil @18:36:31.55 delay 1 second @18:36:33.12 AS.reconfigure ASnorm @18:36:33.24 AS.seek 0ml @18:37:23.97 Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0318h1225ml.pcr @18:37:23.99 Building PCR Slug from ASV with MM2 mix, PP3 primer @18:37:24.00 Priming with carrier @18:37:24.01 ATV.dial ARV @18:37:24.68 ARV.dial :carrier @18:37:25.03 AS.seek 0.5ml @18:37:50.58 AFV.select :carrierSolution @18:37:50.70 delay 2 seconds @18:37:52.73 AS.reconfigure ASconfig @18:37:52.78 AS.seek 1ml @18:38:21.82 delay 1 second @18:38:22.83 AFV.select :holdingCoil @18:38:22.94 delay 1 second @18:38:23.96 ATV.dial :waste @18:38:25.48 AS.reconfigure ASnorm @18:38:25.54 AS.seek :empty @18:39:16.28 AFV.select :carrierSolution @18:39:16.40 delay 2 seconds @18:39:18.43 AS.reconfigure ASconfig @18:39:18.48 AS.seek 0.5ml @18:39:47.63 delay 1 second @18:39:48.64 AFV.select :holdingCoil @18:39:48.75 Building Slug @18:39:48.76 ATV.dial :ARV @18:39:50.26 delay 2 seconds @18:39:52.28 ARV.dial :air1 @18:39:52.66 delay 2 seconds @18:39:54.75 AS.reconfigure ASslow2 @18:39:54.80 AS.seek 0.506ml @18:40:01.07 delay 2 seconds @18:40:03.09 ARV.dial :MM2 @18:40:04.48 delay 2 seconds @18:40:06.51 Adding MM2 @18:40:06.60 AS.seek 0.524ml @18:40:24.82 delay 25 seconds @18:40:49.84 ARV.dial :PP3 @18:40:50.23 Adding PP3 @18:40:50.25 delay 2 seconds @18:40:52.33 AS.seek 0.53ml @18:40:58.79 delay 10 seconds @18:41:08.81 ARV.dial :air2 @18:41:09.18 adding ARV air @18:41:09.20 delay 2 seconds @18:41:11.28 AS.seek 0.536ml @18:41:17.53 delay 5 seconds @18:41:22.55 ARV.dial :carrier @18:41:23.74 delay 7 seconds @18:41:30.83 AS.seek 0.594ml @18:42:29.54 delay 5 seconds @18:42:34.56 ATV.dial :ASV @18:42:34.95 delay 2 seconds @18:42:37.04 AS.seek 0.6ml @18:42:43.35 delay 4 seconds @18:42:47.37 ATV.dial ARV @18:42:47.90 delay 2 seconds @18:42:49.92 ARV.dial :carrier @18:42:49.94 delay 2 seconds @18:42:52.03 AS.seek 0.65ml @18:43:42.49 delay 4 seconds @18:43:46.51 ATV.dial PCR @18:43:47.09 ASV.dial :waste1 @18:43:47.10 ARV.dial :air1 @18:43:47.61 Positioning Slug by 0.324ml @18:43:47.70 AS.seek 0.326ml @18:49:13.93 delay 5 seconds @18:49:18.95 ATV.dial ARV @18:49:19.67 ASV.dial :PCRlock @18:49:20.06 delay 5 seconds @18:49:25.09 Running Rp Profile60C300 thermocycling @18:49:25.10 PCR.program Profile60C300 @18:49:25.24 Profile60C300 should complete in < 1:45:21 @18:49:25.25 PCR.run 2:16:41.25 @20:11:25.12 PCR.off @20:11:26.68 PCR for Rp completed -- closed /var/log/esp/Rp18oct0318h1225ml.pcr @20:11:26.76 Cleaning PCR @20:11:26.77 AFV.select :carrierSolution @20:11:26.88 delay 2 seconds @20:11:28.91 AS.reconfigure ASconfig @20:11:28.96 AS.seek 0.5ml @20:11:39.24 delay 1 second @20:11:40.26 AFV.select :holdingCoil @20:11:40.37 ASV.dial :waste1 @20:11:40.75 ATV.dial :air3 @20:11:41.92 AS.reconfigure ASslow1 @20:11:41.98 AS.seek 0.51ml @20:11:44.27 delay 2 seconds @20:11:46.29 ATV.dial :bleach @20:11:46.89 AS.seek 0.81ml @20:12:48.13 delay 3 seconds @20:12:51.15 ATV.dial :air3 @20:12:51.68 delay 3 seconds @20:12:54.77 AS.seek 0.835ml @20:13:00.09 delay 3 seconds @20:13:03.12 ATV.dial :bleach @20:13:03.72 AS.seek 0.82ml @20:13:07.02 delay 3 seconds @20:13:10.04 ARV.dial :waste @20:13:10.61 delay 3 seconds @20:13:13.63 ATV.dial :ARV @20:13:14.47 AS.reconfigure ASconfig @20:13:14.53 AS.seek 0.47ml @20:13:34.93 AFV.select :carrierSolution @20:13:35.05 delay 2 seconds @20:13:37.08 AS.seek 1ml @20:14:07.85 delay 1 second @20:14:08.86 AFV.select :holdingCoil @20:14:08.97 delay 1 second @20:14:10.00 AS.reconfigure ASfast @20:14:10.05 AS.seek 0ml @20:14:37.40 AFV.select :carrierSolution @20:14:37.52 delay 2 seconds @20:14:39.55 AS.reconfigure ASconfig @20:14:39.60 AS.seek 1ml @20:15:37.40 delay 1 second @20:15:38.41 AFV.select :holdingCoil @20:15:38.52 delay 1 second @20:15:39.56 AS.reconfigure ASfast @20:15:39.65 AS.seek 0ml @20:16:07.01 AFV.select :carrierSolution @20:16:07.13 delay 2 seconds @20:16:09.16 AS.reconfigure ASconfig @20:16:09.21 AS.seek 1ml @20:17:07.01 delay 1 second @20:17:08.02 AFV.select :holdingCoil @20:17:08.13 delay 1 second @20:17:09.16 AS.reconfigure ASfast @20:17:09.22 AS.seek 0ml @20:17:36.56 ARV.dial between carrier and air1 @20:17:37.05 ATV.dial :air3 @20:17:38.25 AS.reconfigure ASslow1 @20:17:38.30 AS.seek 0.01ml @20:17:40.66 delay 2 seconds @20:17:42.67 ATV.dial :bleach @20:17:43.26 AS.seek 0.81ml @20:20:24.40 delay 3 seconds @20:20:27.42 ATV.dial :air3 @20:20:27.94 delay 3 seconds @20:20:31.03 AS.seek 0.835ml @20:20:36.31 delay 3 seconds @20:20:39.33 ATV.dial :bleach @20:20:39.94 AS.seek 0.82ml @20:20:43.24 delay 3 seconds @20:20:46.26 ATV.dial PCR @20:20:46.65 AS.reconfigure ASconfig @20:20:46.70 AS.seek :empty @20:21:34.15 PCR.program :bleachProfile @20:21:34.22 PCR program should complete in < 2:45 @20:21:34.23 PCR.run 10:00 @20:24:42.11 PCR.off @20:24:42.13 AFV.select :carrierSolution @20:24:42.25 delay 2 seconds @20:24:44.28 AS.seek 1ml @20:25:42.07 delay 1 second @20:25:43.08 AFV.select :holdingCoil @20:25:43.19 delay 1 second @20:25:44.22 AS.reconfigure ASfast @20:25:44.27 AS.seek 0ml @20:26:11.62 delay 3 seconds @20:26:14.64 AFV.select :carrierSolution @20:26:14.75 delay 2 seconds @20:26:16.78 AS.reconfigure ASconfig @20:26:16.83 AS.seek 1ml @20:27:14.67 delay 1 second @20:27:15.68 AFV.select :holdingCoil @20:27:15.79 delay 1 second @20:27:16.82 AS.reconfigure ASfast @20:27:16.87 AS.seek 0ml @20:27:44.23 delay 3 seconds @20:27:47.25 AFV.select :carrierSolution @20:27:47.36 delay 2 seconds @20:27:49.39 AS.reconfigure ASconfig @20:27:49.45 AS.seek 1ml @20:28:47.23 delay 1 second @20:28:48.24 AFV.select :holdingCoil @20:28:48.35 delay 1 second @20:28:49.38 AS.reconfigure ASfast @20:28:49.43 AS.seek 0ml @20:29:16.78 delay 3 seconds @20:29:19.80 AFV.select :carrierSolution @20:29:19.91 delay 2 seconds @20:29:21.95 AS.reconfigure ASconfig @20:29:21.99 AS.seek 1ml @20:30:20.42 delay 1 second @20:30:21.43 AFV.select :holdingCoil @20:30:21.54 delay 1 second @20:30:22.57 AS.reconfigure ASfast @20:30:22.62 AS.seek 0ml @20:30:49.96 delay 3 seconds @20:30:52.98 AFV.select :carrierSolution @20:30:53.09 delay 2 seconds @20:30:55.12 AS.reconfigure ASconfig @20:30:55.18 AS.seek 1ml @20:31:52.96 delay 1 second @20:31:53.97 AFV.select :holdingCoil @20:31:54.08 delay 1 second @20:31:55.11 AS.reconfigure ASnorm @20:31:55.16 AS.seek 0ml @20:32:45.87 Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0320h1225ml.pcr @20:32:45.89 Building PCR Slug from ASV with MM3 mix, PP5 primer @20:32:45.90 Priming with carrier @20:32:45.91 ATV.dial ARV @20:32:46.57 ARV.dial :carrier @20:32:46.92 AS.seek 0.5ml @20:33:12.47 AFV.select :carrierSolution @20:33:12.59 delay 2 seconds @20:33:14.62 AS.reconfigure ASconfig @20:33:14.67 AS.seek 1ml @20:33:43.70 delay 1 second @20:33:44.71 AFV.select :holdingCoil @20:33:44.82 delay 1 second @20:33:45.84 ATV.dial :waste @20:33:47.35 AS.reconfigure ASnorm @20:33:47.40 AS.seek :empty @20:34:38.17 AFV.select :carrierSolution @20:34:38.29 delay 2 seconds @20:34:40.32 AS.reconfigure ASconfig @20:34:40.37 AS.seek 0.5ml @20:35:09.45 delay 1 second @20:35:10.46 AFV.select :holdingCoil @20:35:10.57 Building Slug @20:35:10.58 ATV.dial :ARV @20:35:12.07 delay 2 seconds @20:35:14.09 ARV.dial :air1 @20:35:14.58 delay 2 seconds @20:35:16.66 AS.reconfigure ASslow2 @20:35:16.72 AS.seek 0.506ml @20:35:22.90 delay 2 seconds @20:35:24.92 ARV.dial :MM3 @20:35:25.90 delay 2 seconds @20:35:27.93 Adding MM3 @20:35:28.02 AS.seek 0.524ml @20:35:46.40 delay 25 seconds @20:36:11.42 ARV.dial :PP5 @20:36:11.79 Adding PP5 @20:36:11.81 delay 2 seconds @20:36:13.89 AS.seek 0.53ml @20:36:20.31 delay 10 seconds @20:36:30.34 ARV.dial :air3 @20:36:30.71 adding ARV air @20:36:30.73 delay 2 seconds @20:36:32.81 AS.seek 0.536ml @20:36:39.17 delay 5 seconds @20:36:44.20 ARV.dial :carrier @20:36:45.39 delay 7 seconds @20:36:52.48 AS.seek 0.594ml @20:37:51.31 delay 5 seconds @20:37:56.33 ATV.dial :ASV @20:37:56.72 delay 2 seconds @20:37:58.81 AS.seek 0.6ml @20:38:05.15 delay 4 seconds @20:38:09.17 ATV.dial ARV @20:38:09.79 delay 2 seconds @20:38:11.81 ARV.dial :carrier @20:38:11.83 delay 2 seconds @20:38:13.92 AS.seek 0.65ml @20:39:04.32 delay 4 seconds @20:39:08.34 ATV.dial PCR @20:39:08.93 ASV.dial :waste1 @20:39:08.94 ARV.dial :air1 @20:39:09.31 Positioning Slug by 0.324ml @20:39:09.41 AS.seek 0.326ml @20:44:35.47 delay 5 seconds @20:44:40.49 ATV.dial ARV @20:44:41.33 ASV.dial :PCRlock @20:44:41.72 delay 5 seconds @20:44:46.75 Running Bb Profile60C300 thermocycling @20:44:46.76 PCR.program Profile60C300 @20:44:46.90 Profile60C300 should complete in < 1:45:21 @20:44:46.91 PCR.run 2:16:41.25 @22:06:40.61 PCR.off @22:06:42.20 PCR for Bb completed -- closed /var/log/esp/Bb18oct0320h1225ml.pcr @22:06:42.27 Cleaning PCR @22:06:42.28 AFV.select :carrierSolution @22:06:42.40 delay 2 seconds @22:06:44.43 AS.reconfigure ASconfig @22:06:44.48 AS.seek 0.5ml @22:06:54.79 delay 1 second @22:06:55.80 AFV.select :holdingCoil @22:06:55.91 ASV.dial :waste1 @22:06:56.29 ATV.dial :air3 @22:06:58.12 AS.reconfigure ASslow1 @22:06:58.17 AS.seek 0.51ml @22:07:00.46 delay 2 seconds @22:07:02.47 ATV.dial :bleach @22:07:03.08 AS.seek 0.81ml @22:08:03.69 delay 3 seconds @22:08:06.71 ATV.dial :air3 @22:08:07.24 delay 3 seconds @22:08:10.33 AS.seek 0.835ml @22:08:15.63 delay 3 seconds @22:08:18.65 ATV.dial :bleach @22:08:19.25 AS.seek 0.82ml @22:08:22.54 delay 3 seconds @22:08:25.56 ARV.dial :waste @22:08:26.13 delay 3 seconds @22:08:29.15 ATV.dial :ARV @22:08:29.99 AS.reconfigure ASconfig @22:08:30.05 AS.seek 0.47ml @22:08:50.42 AFV.select :carrierSolution @22:08:50.54 delay 2 seconds @22:08:52.57 AS.seek 1ml @22:09:23.37 delay 1 second @22:09:24.38 AFV.select :holdingCoil @22:09:24.49 delay 1 second @22:09:25.52 AS.reconfigure ASfast @22:09:25.57 AS.seek 0ml @22:09:52.90 AFV.select :carrierSolution @22:09:53.02 delay 2 seconds @22:09:55.05 AS.reconfigure ASconfig @22:09:55.10 AS.seek 1ml @22:10:52.88 delay 1 second @22:10:53.90 AFV.select :holdingCoil @22:10:54.01 delay 1 second @22:10:55.04 AS.reconfigure ASfast @22:10:55.09 AS.seek 0ml @22:11:22.43 AFV.select :carrierSolution @22:11:22.55 delay 2 seconds @22:11:24.58 AS.reconfigure ASconfig @22:11:24.63 AS.seek 1ml @22:12:22.41 delay 1 second @22:12:23.42 AFV.select :holdingCoil @22:12:23.53 delay 1 second @22:12:24.56 AS.reconfigure ASfast @22:12:24.61 AS.seek 0ml @22:12:51.96 ARV.dial between carrier and air1 @22:12:52.45 ATV.dial :air3 @22:12:53.66 AS.reconfigure ASslow1 @22:12:53.71 AS.seek 0.01ml @22:12:56.04 delay 2 seconds @22:12:58.05 ATV.dial :bleach @22:12:58.66 AS.seek 0.81ml @22:15:39.85 delay 3 seconds @22:15:42.87 ATV.dial :air3 @22:15:43.40 delay 3 seconds @22:15:46.49 AS.seek 0.835ml @22:15:51.78 delay 3 seconds @22:15:54.80 ATV.dial :bleach @22:15:55.41 AS.seek 0.82ml @22:15:58.73 delay 3 seconds @22:16:01.75 ATV.dial PCR @22:16:02.13 AS.reconfigure ASconfig @22:16:02.18 AS.seek :empty @22:16:49.64 PCR.program :bleachProfile @22:16:49.71 PCR program should complete in < 2:45 @22:16:49.72 PCR.run 10:00 @22:19:57.60 PCR.off @22:19:57.62 AFV.select :carrierSolution @22:19:57.73 delay 2 seconds @22:19:59.76 AS.seek 1ml @22:20:57.62 delay 1 second @22:20:58.63 AFV.select :holdingCoil @22:20:58.74 delay 1 second @22:20:59.77 AS.reconfigure ASfast @22:20:59.82 AS.seek 0ml @22:21:27.17 delay 3 seconds @22:21:30.19 AFV.select :carrierSolution @22:21:30.30 delay 2 seconds @22:21:32.33 AS.reconfigure ASconfig @22:21:32.38 AS.seek 1ml @22:22:30.23 delay 1 second @22:22:31.24 AFV.select :holdingCoil @22:22:31.35 delay 1 second @22:22:32.37 AS.reconfigure ASfast @22:22:32.42 AS.seek 0ml @22:22:59.76 delay 3 seconds @22:23:02.78 AFV.select :carrierSolution @22:23:02.89 delay 2 seconds @22:23:04.92 AS.reconfigure ASconfig @22:23:04.97 AS.seek 1ml @22:24:03.39 delay 1 second @22:24:04.41 AFV.select :holdingCoil @22:24:04.52 delay 1 second @22:24:05.55 AS.reconfigure ASfast @22:24:05.60 AS.seek 0ml @22:24:32.93 delay 3 seconds @22:24:35.95 AFV.select :carrierSolution @22:24:36.06 delay 2 seconds @22:24:38.09 AS.reconfigure ASconfig @22:24:38.14 AS.seek 1ml @22:25:35.92 delay 1 second @22:25:36.93 AFV.select :holdingCoil @22:25:37.04 delay 1 second @22:25:38.07 AS.reconfigure ASfast @22:25:38.12 AS.seek 0ml @22:26:05.47 delay 3 seconds @22:26:08.49 AFV.select :carrierSolution @22:26:08.60 delay 2 seconds @22:26:10.63 AS.reconfigure ASconfig @22:26:10.68 AS.seek 1ml @22:27:08.47 delay 1 second @22:27:09.48 AFV.select :holdingCoil @22:27:09.59 delay 1 second @22:27:10.62 AS.reconfigure ASnorm @22:27:10.67 AS.seek 0ml @22:28:01.39 Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0322h1225ml.pcr @22:28:01.41 Building PCR Slug from ASV with MM1 mix, PP2 primer @22:28:01.42 Priming with carrier @22:28:01.43 ATV.dial ARV @22:28:02.01 ARV.dial :carrier @22:28:02.38 AS.seek 0.5ml @22:28:27.91 AFV.select :carrierSolution @22:28:28.03 delay 2 seconds @22:28:30.06 AS.reconfigure ASconfig @22:28:30.11 AS.seek 1ml @22:28:59.12 delay 1 second @22:29:00.14 AFV.select :holdingCoil @22:29:00.25 delay 1 second @22:29:01.27 ATV.dial :waste @22:29:02.81 AS.reconfigure ASnorm @22:29:02.86 AS.seek :empty @22:29:53.60 AFV.select :carrierSolution @22:29:53.72 delay 2 seconds @22:29:55.75 AS.reconfigure ASconfig @22:29:55.80 AS.seek 0.5ml @22:30:24.90 delay 1 second @22:30:25.91 AFV.select :holdingCoil @22:30:26.02 Building Slug @22:30:26.03 ATV.dial :ARV @22:30:27.64 delay 2 seconds @22:30:29.66 ARV.dial :air1 @22:30:30.04 delay 2 seconds @22:30:32.12 AS.reconfigure ASslow2 @22:30:32.18 AS.seek 0.506ml @22:30:38.54 delay 2 seconds @22:30:40.55 ARV.dial :MM1 @22:30:41.12 delay 2 seconds @22:30:43.15 Adding MM1 @22:30:43.22 AS.seek 0.524ml @22:31:01.67 delay 25 seconds @22:31:26.69 ARV.dial :PP2 @22:31:27.06 Adding PP2 @22:31:27.07 delay 2 seconds @22:31:29.16 AS.seek 0.53ml @22:31:35.63 delay 10 seconds @22:31:45.66 ARV.dial :air2 @22:31:46.03 adding ARV air @22:31:46.04 delay 2 seconds @22:31:48.13 AS.seek 0.536ml @22:31:54.36 delay 5 seconds @22:31:59.39 ARV.dial :carrier @22:32:00.59 delay 7 seconds @22:32:07.68 AS.seek 0.594ml @22:33:06.15 delay 5 seconds @22:33:11.17 ATV.dial :ASV @22:33:11.58 delay 2 seconds @22:33:13.67 AS.seek 0.6ml @22:33:20.03 delay 4 seconds @22:33:24.05 ATV.dial ARV @22:33:24.58 delay 2 seconds @22:33:26.60 ARV.dial :carrier @22:33:26.62 delay 2 seconds @22:33:28.71 AS.seek 0.65ml @22:34:19.25 delay 4 seconds @22:34:23.28 ATV.dial PCR @22:34:23.84 ASV.dial :waste1 @22:34:23.85 ARV.dial :air1 @22:34:24.23 Positioning Slug by 0.324ml @22:34:24.33 AS.seek 0.326ml @22:39:50.39 delay 5 seconds @22:39:55.42 ATV.dial ARV @22:39:56.25 ASV.dial :PCRlock @22:39:56.64 delay 5 seconds @22:40:01.67 Running Ss Profile60C350 thermocycling @22:40:01.68 PCR.program Profile60C350 @22:40:01.83 Profile60C350 should complete in < 1:48:05 @22:40:01.84 PCR.run 2:20:06.25 @00:02:30.90CEST04-Oct-18 PCR.off @00:02:33.42 PCR for Ss completed -- closed /var/log/esp/Ss18oct0322h1225ml.pcr @00:02:33.49 Cleaning PCR @00:02:33.50 AFV.select :carrierSolution @00:02:33.61 delay 2 seconds @00:02:35.64 AS.reconfigure ASconfig @00:02:35.69 AS.seek 0.5ml @00:02:45.98 delay 1 second @00:02:47.00 AFV.select :holdingCoil @00:02:47.11 ASV.dial :waste1 @00:02:47.49 ATV.dial :air3 @00:02:48.66 AS.reconfigure ASslow1 @00:02:48.72 AS.seek 0.51ml @00:02:51.01 delay 2 seconds @00:02:53.03 ATV.dial :bleach @00:02:53.63 AS.seek 0.81ml @00:03:54.26 delay 3 seconds @00:03:57.28 ATV.dial :air3 @00:03:57.81 delay 3 seconds @00:04:00.89 AS.seek 0.835ml @00:04:06.19 delay 3 seconds @00:04:09.22 ATV.dial :bleach @00:04:09.83 AS.seek 0.82ml @00:04:13.13 delay 3 seconds @00:04:16.15 ARV.dial :waste @00:04:16.74 delay 3 seconds @00:04:19.76 ATV.dial :ARV @00:04:20.60 AS.reconfigure ASconfig @00:04:20.65 AS.seek 0.47ml @00:04:41.05 AFV.select :carrierSolution @00:04:41.17 delay 2 seconds @00:04:43.20 AS.seek 1ml @00:05:13.95 delay 1 second @00:05:14.96 AFV.select :holdingCoil @00:05:15.07 delay 1 second @00:05:16.10 AS.reconfigure ASfast @00:05:16.15 AS.seek 0ml @00:05:43.47 AFV.select :carrierSolution @00:05:43.59 delay 2 seconds @00:05:45.62 AS.reconfigure ASconfig @00:05:45.67 AS.seek 1ml @00:06:43.45 delay 1 second @00:06:44.47 AFV.select :holdingCoil @00:06:44.58 delay 1 second @00:06:45.61 AS.reconfigure ASfast @00:06:45.66 AS.seek 0ml @00:07:12.98 AFV.select :carrierSolution @00:07:13.10 delay 2 seconds @00:07:15.13 AS.reconfigure ASconfig @00:07:15.18 AS.seek 1ml @00:08:12.92 delay 1 second @00:08:13.94 AFV.select :holdingCoil @00:08:14.05 delay 1 second @00:08:15.08 AS.reconfigure ASfast @00:08:15.13 AS.seek 0ml @00:08:42.45 ARV.dial between carrier and air1 @00:08:42.93 ATV.dial :air3 @00:08:44.13 AS.reconfigure ASslow1 @00:08:44.18 AS.seek 0.01ml @00:08:46.47 delay 2 seconds @00:08:48.49 ATV.dial :bleach @00:08:49.10 AS.seek 0.81ml @00:11:30.22 delay 3 seconds @00:11:33.25 ATV.dial :air3 @00:11:33.78 delay 3 seconds @00:11:36.86 AS.seek 0.835ml @00:11:42.15 delay 3 seconds @00:11:45.17 ATV.dial :bleach @00:11:45.77 AS.seek 0.82ml @00:11:49.14 delay 3 seconds @00:11:52.16 ATV.dial PCR @00:11:52.54 AS.reconfigure ASconfig @00:11:52.59 AS.seek :empty @00:12:40.06 PCR.program :bleachProfile @00:12:40.13 PCR program should complete in < 2:45 @00:12:40.14 PCR.run 10:00 @00:15:48.40 PCR.off @00:15:49.03 AFV.select :carrierSolution @00:15:49.16 delay 2 seconds @00:15:51.19 AS.seek 1ml @00:16:49.03 delay 1 second @00:16:50.04 AFV.select :holdingCoil @00:16:50.15 delay 1 second @00:16:51.18 AS.reconfigure ASfast @00:16:51.23 AS.seek 0ml @00:17:18.57 delay 3 seconds @00:17:21.59 AFV.select :carrierSolution @00:17:21.70 delay 2 seconds @00:17:23.73 AS.reconfigure ASconfig @00:17:23.83 AS.seek 1ml @00:18:21.58 delay 1 second @00:18:22.59 AFV.select :holdingCoil @00:18:22.70 delay 1 second @00:18:23.73 AS.reconfigure ASfast @00:18:23.78 AS.seek 0ml @00:18:51.12 delay 3 seconds @00:18:54.14 AFV.select :carrierSolution @00:18:54.25 delay 2 seconds @00:18:56.28 AS.reconfigure ASconfig @00:18:56.33 AS.seek 1ml @00:19:54.12 delay 1 second @00:19:55.13 AFV.select :holdingCoil @00:19:55.24 delay 1 second @00:19:56.27 AS.reconfigure ASfast @00:19:56.32 AS.seek 0ml @00:20:23.67 delay 3 seconds @00:20:26.69 AFV.select :carrierSolution @00:20:26.80 delay 2 seconds @00:20:28.83 AS.reconfigure ASconfig @00:20:28.89 AS.seek 1ml @00:21:26.67 delay 1 second @00:21:27.68 AFV.select :holdingCoil @00:21:27.79 delay 1 second @00:21:28.82 AS.reconfigure ASfast @00:21:28.87 AS.seek 0ml @00:21:56.22 delay 3 seconds @00:21:59.24 AFV.select :carrierSolution @00:21:59.35 delay 2 seconds @00:22:01.38 AS.reconfigure ASconfig @00:22:01.43 AS.seek 1ml @00:22:59.19 delay 1 second @00:23:00.20 AFV.select :holdingCoil @00:23:00.31 delay 1 second @00:23:01.34 AS.reconfigure ASnorm @00:23:01.39 AS.seek 0ml @00:23:52.13 Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0400h1225ml.pcr @00:23:52.14 Building PCR Slug from ASV with MM1 mix, PP1 primer @00:23:52.16 Priming with carrier @00:23:52.17 ATV.dial ARV @00:23:52.73 ARV.dial :carrier @00:23:53.07 AS.seek 0.5ml @00:24:18.60 AFV.select :carrierSolution @00:24:18.72 delay 2 seconds @00:24:20.75 AS.reconfigure ASconfig @00:24:20.80 AS.seek 1ml @00:24:49.81 delay 1 second @00:24:50.82 AFV.select :holdingCoil @00:24:50.93 delay 1 second @00:24:51.95 ATV.dial :waste @00:24:53.48 AS.reconfigure ASnorm @00:24:53.53 AS.seek :empty @00:25:44.26 AFV.select :carrierSolution @00:25:44.38 delay 2 seconds @00:25:46.41 AS.reconfigure ASconfig @00:25:46.46 AS.seek 0.5ml @00:26:15.55 delay 1 second @00:26:16.57 AFV.select :holdingCoil @00:26:16.68 Building Slug @00:26:16.69 ATV.dial :ARV @00:26:18.30 delay 2 seconds @00:26:20.32 ARV.dial :air1 @00:26:20.81 delay 2 seconds @00:26:22.89 AS.reconfigure ASslow2 @00:26:22.94 AS.seek 0.506ml @00:26:29.36 delay 2 seconds @00:26:31.37 ARV.dial :MM1 @00:26:31.95 delay 2 seconds @00:26:33.98 Adding MM1 @00:26:34.05 AS.seek 0.524ml @00:26:52.31 delay 25 seconds @00:27:17.33 ARV.dial :PP1 @00:27:17.70 Adding PP1 @00:27:17.71 delay 2 seconds @00:27:19.79 AS.seek 0.53ml @00:27:26.11 delay 10 seconds @00:27:36.14 ARV.dial :air1 @00:27:36.53 adding ARV air @00:27:36.54 delay 2 seconds @00:27:38.63 AS.seek 0.536ml @00:27:44.89 delay 5 seconds @00:27:49.91 ARV.dial :carrier @00:27:50.30 delay 7 seconds @00:27:57.39 AS.seek 0.594ml @00:28:56.22 delay 5 seconds @00:29:01.24 ATV.dial :ASV @00:29:01.64 delay 2 seconds @00:29:03.73 AS.seek 0.6ml @00:29:10.16 delay 4 seconds @00:29:14.19 ATV.dial ARV @00:29:14.72 delay 2 seconds @00:29:16.74 ARV.dial :carrier @00:29:16.76 delay 2 seconds @00:29:18.85 AS.seek 0.65ml @00:30:09.41 delay 4 seconds @00:30:13.43 ATV.dial PCR @00:30:14.61 ASV.dial :waste1 @00:30:14.66 ARV.dial :air1 @00:30:15.03 Positioning Slug by 0.324ml @00:30:15.12 AS.seek 0.326ml @00:35:41.24 delay 5 seconds @00:35:46.26 ATV.dial ARV @00:35:46.99 ASV.dial :PCRlock @00:35:47.38 delay 5 seconds @00:35:52.41 Running IPc ProfileIPc thermocycling @00:35:52.42 PCR.program ProfileIPc @00:35:52.57 ProfileIPc should complete in < 1:50:49 @00:35:52.58 PCR.run 2:23:31.25 @02:00:02.70 PCR.off @02:00:05.33 PCR for IPc completed -- closed /var/log/esp/IPc18oct0400h1225ml.pcr @02:00:05.35 Full MFB Decontamination @02:00:05.36 Decon extraction column and decon AIV @02:00:05.38 SPE.hold 2:46:40,55C @02:00:05.44 ASV.dial :SPEdelivery @02:00:06.11 delay 20 seconds @02:00:26.13 AFV.select :carrierSolution @02:00:26.24 delay 2 seconds @02:00:28.27 AS.reconfigure ASconfig @02:00:28.32 AS.seek 0.5ml @02:00:38.64 delay 1 second @02:00:39.65 AFV.select :holdingCoil @02:00:39.76 ATV.dial :air1 @02:00:41.52 AS.reconfigure ASslow1 @02:00:41.57 AS.seek 0.52ml @02:00:45.86 ATV.dial :ASV @02:00:47.36 ASV.dial :SPErecovery @02:00:47.99 AS.seek 0.62ml @02:01:08.36 ATV.dial :waste @02:01:10.17 AS.reconfigure ASnorm @02:01:10.22 AS.seek :empty @02:01:41.83 AFV.select :holdingCoil @02:01:41.85 ATV.dial :waste @02:01:41.87 AS.reconfigure ASconfig @02:01:41.91 AS.seek 0ml @02:01:42.24 AFV.select :carrierSolution @02:01:42.36 delay 2 seconds @02:01:44.39 AS.seek 0.5ml @02:02:13.42 delay 1 second @02:02:14.43 AFV.select :holdingCoil @02:02:14.54 ATV.dial :air3 @02:02:15.28 AS.reconfigure ASslow1 @02:02:15.34 AS.seek 0.52ml @02:02:19.61 ATV.dial :air3 @02:02:19.70 AS.seek 0.53ml @02:02:21.97 delay 2 seconds @02:02:23.99 ATV.dial :bleach @02:02:24.46 AS.seek 0.71ml @02:03:00.92 delay 3 seconds @02:03:03.94 ATV.dial :air3 @02:03:04.47 delay 3 seconds @02:03:07.55 AS.seek 0.735ml @02:03:12.87 delay 3 seconds @02:03:16.48 ATV.dial :bleach @02:03:17.08 AS.seek 0.72ml @02:03:20.48 delay 3 seconds @02:03:23.51 ATV.dial ASV @02:03:24.46 ASV.dial :SPErecovery @02:03:24.55 AS.seek 0.365ml @02:04:36.21 ATV.dial :waste @02:04:37.78 AS.reconfigure ASnorm @02:04:37.83 AS.seek :empty @02:04:56.61 ATV.dial :air3 @02:04:57.25 AS.reconfigure ASslow1 @02:04:57.30 AS.seek 0.009ml @02:04:59.65 delay 2 seconds @02:05:01.66 ATV.dial :bleach @02:05:02.13 AS.seek 0.579ml @02:06:57.03 delay 3 seconds @02:07:00.05 ATV.dial :air3 @02:07:00.58 delay 3 seconds @02:07:03.67 AS.seek 0.604ml @02:07:08.98 delay 3 seconds @02:07:12.00 ATV.dial :bleach @02:07:12.60 AS.seek 0.589ml @02:07:15.95 delay 3 seconds @02:07:18.97 ATV.dial :ASV @02:07:20.02 ASV.dial :wasteR @02:07:20.49 AS.seek 0ml @02:09:19.19 delay 1:00 @02:10:19.21 ATV.dial ASV @02:10:19.23 AFV.select :carrierSolution @02:10:19.33 delay 2 seconds @02:10:21.36 AS.reconfigure ASconfig @02:10:21.41 AS.seek 1ml @02:11:19.17 delay 1 second @02:11:20.18 AFV.select :holdingCoil @02:11:20.29 delay 1 second @02:11:21.31 ASV.dial :wasteR @02:11:21.33 AS.reconfigure ASslow1 @02:11:21.38 AS.seek 0ml @02:14:42.75 delay 10 seconds @02:14:52.77 ATV.dial :air3 @02:14:54.16 AS.seek 0.01ml @02:14:56.47 delay 2 seconds @02:14:58.48 ATV.dial :bleach @02:14:59.09 AS.seek 0.58ml @02:16:53.95 delay 3 seconds @02:16:56.97 ATV.dial :air3 @02:16:57.50 delay 3 seconds @02:17:00.59 AS.seek 0.605ml @02:17:05.87 delay 3 seconds @02:17:08.89 ATV.dial :bleach @02:17:09.49 AS.seek 0.59ml @02:17:12.83 delay 3 seconds @02:17:15.85 ATV.dial ASV @02:17:16.90 ASV.dial :SPEdelivery @02:17:17.27 AS.seek 0ml @02:19:16.20 delay 1:00 @02:20:16.22 AFV.select :carrierSolution @02:20:16.33 delay 2 seconds @02:20:18.36 AS.reconfigure ASconfig @02:20:18.41 AS.seek 1ml @02:21:16.17 delay 1 second @02:21:17.18 AFV.select :holdingCoil @02:21:17.29 delay 1 second @02:21:18.31 ATV.dial ASV @02:21:18.33 ASV.dial :SPEdelivery @02:21:18.34 AS.reconfigure ASslow1 @02:21:18.39 AS.seek 0ml @02:24:39.78 delay 10 seconds @02:24:49.81 ATV.dial :air2 @02:24:50.58 AS.reconfigure ASslow0 @02:24:50.63 AS.seek 0.8ml @02:26:11.42 delay 3 seconds @02:26:14.44 ATV.dial :ASV @02:26:15.36 ASV.dial :SPEdelivery @02:26:15.38 AS.reconfigure ASslow1 @02:26:15.43 AS.seek 0.03ml @02:28:50.55 delay 20 seconds @02:29:10.58 ATV.dial :waste,via: :air3 @02:29:12.16 AS.reconfigure ASconfig @02:29:12.21 AS.seek :empty @02:29:14.31 SPE.coast @02:29:14.40 Removing Bleach from Mixing Coil @02:29:14.41 AFV.select :carrierSolution @02:29:14.52 delay 2 seconds @02:29:16.55 AS.seek 0.5ml @02:29:45.66 delay 1 second @02:29:46.67 AFV.select :holdingCoil @02:29:46.78 ATV.dial :ASV @02:29:48.35 ASV.dial :SPErecovery @02:29:49.60 AS.reconfigure ASslow1 @02:29:49.66 AS.seek 0.73ml @02:30:36.18 ASV.dial :wasteD @02:30:36.74 AS.reconfigure ASnorm @02:30:36.79 AS.seek 0ml @02:31:13.92 AFV.select :carrierSolution @02:31:14.04 delay 2 seconds @02:31:16.07 AS.reconfigure ASconfig @02:31:16.12 AS.seek 0.5ml @02:31:45.18 delay 1 second @02:31:46.19 AFV.select :holdingCoil @02:31:46.30 ATV.dial ASV @02:31:46.31 ASV.dial :SPErecovery @02:31:46.93 AS.reconfigure ASslow0 @02:31:46.98 AS.seek 0.3ml @02:32:07.43 ATV.dial :air2 @02:32:08.42 AS.reconfigure ASslow1 @02:32:08.47 AS.seek 0.32ml @02:32:12.80 ATV.dial ASV @02:32:13.77 AS.reconfigure ASslow0 @02:32:13.83 AS.seek 0.57ml @02:32:39.29 ASV.dial :wasteD @02:32:39.85 AS.reconfigure ASnorm @02:32:39.90 AS.seek 0ml @02:33:08.96 AFV.select :carrierSolution @02:33:09.08 delay 2 seconds @02:33:11.11 AS.reconfigure ASconfig @02:33:11.16 AS.seek 0.5ml @02:33:40.22 delay 1 second @02:33:41.23 AFV.select :holdingCoil @02:33:41.34 ATV.dial ASV @02:33:41.35 ASV.dial :SPErecovery @02:33:41.98 AS.reconfigure ASslow0 @02:33:42.03 AS.seek 0.3ml @02:34:02.45 ATV.dial :air2 @02:34:03.43 AS.reconfigure ASslow1 @02:34:03.48 AS.seek 0.32ml @02:34:07.81 ATV.dial ASV @02:34:08.77 AS.reconfigure ASslow0 @02:34:08.82 AS.seek 0.57ml @02:34:34.29 ASV.dial :wasteD @02:34:34.85 AS.reconfigure ASnorm @02:34:34.90 AS.seek 0ml @02:35:03.96 ATV.dial :air2 @02:35:04.88 AS.reconfigure ASslow0 @02:35:04.94 AS.seek 0.3ml @02:35:35.43 ATV.dial :ASV @02:35:36.32 ASV.dial :wasteD @02:35:36.35 AS.reconfigure ASconfig @02:35:36.39 AS.seek 0.05ml @02:35:51.07 ATV.dial :waste @02:35:52.88 AS.seek 0ml @02:35:56.12 ASV.dial :SPEdelivery @02:35:57.05 deconLysateINT @02:35:57.06 ATV.dial :air3 @02:35:57.81 AS.reconfigure ASslow1 @02:35:57.86 AS.seek 0.01ml @02:36:00.21 delay 2 seconds @02:36:02.22 ATV.dial :bleach @02:36:02.68 AS.seek 0.96ml @02:39:14.01 delay 3 seconds @02:39:17.03 ATV.dial :air3 @02:39:17.57 delay 3 seconds @02:39:20.66 AS.seek 0.985ml @02:39:25.95 delay 3 seconds @02:39:28.97 ATV.dial :bleach @02:39:29.58 AS.seek 0.97ml @02:39:32.90 delay 3 seconds @02:39:35.93 ATV.dial AIV @02:39:37.08 AIV.dial :coilToMFB @02:39:37.09 AWV.select :waste @02:39:37.10 AFV.select :carrierSolution @02:39:37.22 delay 2 seconds @02:39:39.25 AS.reconfigure ASconfig @02:39:39.30 AS.seek 1ml @02:39:41.33 delay 1 second @02:39:42.34 AFV.select :holdingCoil @02:39:42.45 delay 1 second @02:39:43.48 AS.reconfigure ASslow0 @02:39:43.52 AS.seek :empty @02:41:24.43 AFV.select :carrierSolution @02:41:24.55 delay 2 seconds @02:41:26.58 AS.reconfigure ASconfig @02:41:26.63 AS.seek 1ml @02:42:24.48 delay 1 second @02:42:25.49 AFV.select :holdingCoil @02:42:25.60 delay 1 second @02:42:26.63 AS.reconfigure ASslow0 @02:42:26.67 AS.seek :empty @02:44:08.28 AFV.select :carrierSolution @02:44:08.40 delay 2 seconds @02:44:10.43 AS.reconfigure ASconfig @02:44:10.48 AS.seek 1ml @02:45:08.34 delay 1 second @02:45:09.35 AFV.select :holdingCoil @02:45:09.46 delay 1 second @02:45:10.49 AS.reconfigure ASslow0 @02:45:10.54 AS.seek :empty @02:46:51.48 ATV.dial :air2 @02:46:52.12 AS.reconfigure ASslow1 @02:46:52.17 AS.seek 0.009ml @02:46:54.49 AS.reconfigure ASslow0 @02:46:54.54 AS.seek 1ml @02:48:34.51 ATV.dial AIV @02:48:35.35 AS.seek 0.02ml @02:50:14.20 ATV.dial :waste @02:50:15.71 AS.seek 0ml @02:50:18.03 AWV.select :air @02:50:18.21 Cleaning PCR @02:50:18.22 AFV.select :carrierSolution @02:50:18.32 delay 2 seconds @02:50:20.35 AS.reconfigure ASconfig @02:50:20.40 AS.seek 0.5ml @02:50:49.43 delay 1 second @02:50:50.44 AFV.select :holdingCoil @02:50:50.55 ASV.dial :waste1 @02:50:50.90 ATV.dial :air3 @02:50:51.55 AS.reconfigure ASslow1 @02:50:51.60 AS.seek 0.51ml @02:50:53.88 delay 2 seconds @02:50:55.89 ATV.dial :bleach @02:50:56.33 AS.seek 0.81ml @02:51:56.90 delay 3 seconds @02:51:59.92 ATV.dial :air3 @02:52:00.52 delay 3 seconds @02:52:03.60 AS.seek 0.835ml @02:52:08.92 delay 3 seconds @02:52:11.94 ATV.dial :bleach @02:52:12.55 AS.seek 0.82ml @02:52:15.90 delay 3 seconds @02:52:18.92 ARV.dial :waste @02:52:19.49 delay 3 seconds @02:52:22.51 ATV.dial :ARV @02:52:23.33 AS.reconfigure ASconfig @02:52:23.38 AS.seek 0.47ml @02:52:43.80 AFV.select :carrierSolution @02:52:43.92 delay 2 seconds @02:52:45.95 AS.seek 1ml @02:53:16.74 delay 1 second @02:53:17.75 AFV.select :holdingCoil @02:53:17.86 delay 1 second @02:53:18.89 AS.reconfigure ASfast @02:53:18.94 AS.seek 0ml @02:53:46.28 AFV.select :carrierSolution @02:53:46.40 delay 2 seconds @02:53:48.43 AS.reconfigure ASconfig @02:53:48.48 AS.seek 1ml @02:54:46.26 delay 1 second @02:54:47.28 AFV.select :holdingCoil @02:54:47.38 delay 1 second @02:54:48.41 AS.reconfigure ASfast @02:54:48.46 AS.seek 0ml @02:55:15.80 AFV.select :carrierSolution @02:55:15.92 delay 2 seconds @02:55:17.95 AS.reconfigure ASconfig @02:55:18.00 AS.seek 1ml @02:56:15.82 delay 1 second @02:56:16.83 AFV.select :holdingCoil @02:56:16.94 delay 1 second @02:56:17.97 AS.reconfigure ASfast @02:56:18.02 AS.seek 0ml @02:56:45.36 ARV.dial between carrier and air1 @02:56:45.85 ATV.dial :air3 @02:56:47.05 AS.reconfigure ASslow1 @02:56:47.10 AS.seek 0.01ml @02:56:49.40 delay 2 seconds @02:56:51.41 ATV.dial :bleach @02:56:52.02 AS.seek 0.81ml @02:59:33.14 delay 3 seconds @02:59:36.16 ATV.dial :air3 @02:59:36.70 delay 3 seconds @02:59:39.78 AS.seek 0.835ml @02:59:45.06 delay 3 seconds @02:59:48.08 ATV.dial :bleach @02:59:48.67 AS.seek 0.82ml @02:59:51.98 delay 3 seconds @02:59:55.00 ATV.dial PCR @02:59:55.39 AS.reconfigure ASconfig @02:59:55.44 AS.seek :empty @03:00:42.89 PCR.program :bleachProfile @03:00:42.95 PCR program should complete in < 2:45 @03:00:42.96 PCR.run 10:00 @03:04:03.60 PCR.off @03:04:03.62 AFV.select :carrierSolution @03:04:03.73 delay 2 seconds @03:04:05.76 AS.seek 1ml @03:05:03.61 delay 1 second @03:05:04.62 AFV.select :holdingCoil @03:05:04.73 delay 1 second @03:05:05.76 AS.reconfigure ASfast @03:05:05.81 AS.seek 0ml @03:05:33.17 delay 3 seconds @03:05:36.19 AFV.select :carrierSolution @03:05:36.30 delay 2 seconds @03:05:38.33 AS.reconfigure ASconfig @03:05:38.39 AS.seek 1ml @03:06:36.17 delay 1 second @03:06:37.18 AFV.select :holdingCoil @03:06:37.29 delay 1 second @03:06:38.32 AS.reconfigure ASfast @03:06:38.37 AS.seek 0ml @03:07:05.70 delay 3 seconds @03:07:08.72 AFV.select :carrierSolution @03:07:08.83 delay 2 seconds @03:07:10.86 AS.reconfigure ASconfig @03:07:10.91 AS.seek 1ml @03:08:08.75 delay 1 second @03:08:09.76 AFV.select :holdingCoil @03:08:09.87 delay 1 second @03:08:10.90 AS.reconfigure ASfast @03:08:10.95 AS.seek 0ml @03:08:38.31 delay 3 seconds @03:08:41.33 AFV.select :carrierSolution @03:08:41.44 delay 2 seconds @03:08:43.47 AS.reconfigure ASconfig @03:08:43.53 AS.seek 1ml @03:09:41.37 delay 1 second @03:09:42.38 AFV.select :holdingCoil @03:09:42.49 delay 1 second @03:09:43.52 AS.reconfigure ASfast @03:09:43.57 AS.seek 0ml @03:10:10.90 delay 3 seconds @03:10:13.92 AFV.select :carrierSolution @03:10:14.03 delay 2 seconds @03:10:16.06 AS.reconfigure ASconfig @03:10:16.11 AS.seek 1ml @03:11:13.90 delay 1 second @03:11:14.91 AFV.select :holdingCoil @03:11:15.02 delay 1 second @03:11:16.05 AS.reconfigure ASnorm @03:11:16.10 AS.seek 0ml @03:12:06.81 AFV.select :carrierSolution @03:12:06.92 delay 2 seconds @03:12:08.95 AS.reconfigure ASconfig @03:12:09.00 AS.seek 1ml @03:13:06.80 delay 1 second @03:13:07.81 AFV.select :holdingCoil @03:13:07.92 delay 1 second @03:13:08.94 ATV.dial :waste @03:13:10.17 AS.reconfigure ASnorm @03:13:10.22 AS.seek 0.5ml @03:13:35.79 ATV.dial :waste2 @03:13:36.98 AS.seek 0ml @03:14:02.51 AS.coast @03:14:02.55 SS.coast @03:14:02.59 CC.coast @03:14:02.63 CS.coast @03:14:02.67 PC.coast @03:14:02.71 PS.coast @03:14:02.75 Forearm.coast @03:14:02.79 Elbow.coast @03:14:03.18 Gate.power :core,:OFF @03:14:03.19 Gate.power :raw,:OFF @03:14:03.20 Shutdown @03:14:03.22 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @10:09:25.05 Session Begun @10:09:34.49 -> ESP.ready? @10:09:46.41 -> ESP.ready! @10:09:46.47 Powering core bus @10:09:51.60 Gate.power :raw,:ON @10:09:52.30 SPE.coast @10:09:52.40 SPE.configure SPEconfig @10:09:52.51 AS.configure ASconfig @10:09:52.64 SS.configure SSconfig @10:09:52.77 CC.configure CCconfig @10:09:52.90 CS.configure CSconfig @10:09:53.02 PC.configure PCconfig @10:09:53.14 PS.configure PSconfig @10:09:53.29 Forearm.configure LooseForearm @10:09:53.42 Elbow.configure LooseElbow @10:09:53.57 SC.configure SCloose @10:09:53.68 SE.configure SEconfig @10:09:53.76 CH.coast @10:09:53.84 CH.configure CHconfig @10:09:53.90 PH.coast @10:09:53.99 PH.configure PHconfig @10:09:54.05 PCR.off @10:09:54.10 PCR.pidtemp= 50,0.1,0,300,25,25 @10:09:54.14 PCR.caltemp= 2.7073,0.0373,0 @10:09:54.18 All dwarf controllers running firmware version 3.75 @10:09:54.46 Intake.select :close @10:09:54.51 Exhaust.select :close @10:09:54.53 CTV.dial :air @10:09:54.63 PSV.dial :air @10:09:54.79 CSV.dial :waste1 @10:09:54.81 Forearm.reconfigure LooseForearm @10:09:54.94 Forearm.home.seek :retract @10:09:55.05 SSV.dial :spit,avoiding: :exhaust @10:09:55.20 PS.reconfigure PSconfig @10:09:55.27 PS.home.seek 0ml @10:09:55.69 Hand.close @10:09:55.72 Elbow.jog 1500 @10:09:55.91 CS.reconfigure CSfast @10:09:55.95 SS.reconfigure SSconfig @10:09:56.07 CS.home.seek 0ml @10:09:56.12 SS.home.seek 0ml @10:09:56.41 Elbow.reconfigure LooseElbow @10:09:56.48 Elbow.home.seek :clear @10:09:59.90 SE.reconfigure SEconfig @10:09:59.97 SE.home.seek :down @10:10:00.60 SC.reconfigure SCsloppy @10:10:00.67 SC.home.seek :initial @10:10:02.88 Forearm.seek :retract @10:10:03.00 Elbow.seek :hammer @10:10:04.35 Forearm.reconfigure TightForearm @10:10:04.46 Forearm.seek 5 + 350 counts @10:10:04.47 PS.coast @10:10:05.64 Forearm.seek :retract @10:10:06.87 PC.reconfigure PCconfig @10:10:06.89 CC.reconfigure CCconfig @10:10:06.99 PC.home.seek :open @10:10:07.10 CC.home.seek :open @10:10:25.57 -> MFB.ready? @10:10:30.62 -> MFB.ready! @10:10:30.76 Readying the MFB @10:10:30.77 Homing MFB Syringe @10:10:30.79 AFV.select :holdingCoil @10:10:30.98 ATV.dial :air2 @10:10:31.52 AS.reconfigure ASconfig @10:10:31.60 AS.home.seek :home @10:10:37.14 AFV.select :carrierSolution @10:10:37.28 delay 2 seconds @10:10:39.31 AS.seek 1ml @10:11:32.32 delay 1 second @10:11:33.33 AFV.select :holdingCoil @10:11:33.47 delay 1 second @10:11:34.50 ATV.dial :waste @10:11:35.49 AS.reconfigure ASnorm @10:11:35.57 AS.home.seek :empty @10:12:28.15 ATV.dial :waste @10:12:28.17 ARV.dial between carrier and air1 @10:12:28.30 ASV.dial :waste1 @10:12:38.37 -> ATV @10:12:44.81 -> ATV.ARV @10:12:45.43 ATV.dial ARV @10:12:52.27 -> ARV.air3 @10:12:52.29 ARV.dial :air3 @10:13:16.75 -> AS.pull 0.2, ASslow1 @10:13:16.86 AS.reconfigure ASslow1 @10:13:16.94 AS.seek 0.199ml @10:14:04.22 -> ATV @10:14:13.64 -> ARV.PP5 @10:14:13.66 ARV.dial :PP5 @10:14:31.12 -> AS.pull 0.05, ASslow1 @10:14:31.24 AS.seek 0.249ml @10:15:08.91 -> ARV.air3 @10:15:08.94 ARV.dial :air3 @10:15:36.79 -> AS.pull 0.15, ASslow1 @10:15:36.90 AS.seek 0.399ml @10:16:16.50 -> ATV.waste @10:16:16.52 ATV.dial :waste @10:16:23.42 -> AS.empty @10:16:23.45 AS.reconfigure ASconfig @10:16:23.53 AS.seek :empty @10:17:04.70 -> pcrDecon @10:17:04.79 Cleaning PCR @10:17:04.81 AFV.select :carrierSolution @10:17:04.96 delay 2 seconds @10:17:06.99 AS.seek 0.5ml @10:17:36.07 delay 1 second @10:17:37.08 AFV.select :holdingCoil @10:17:37.21 ASV.dial :waste1 @10:17:37.23 ATV.dial :air3 @10:17:38.03 AS.reconfigure ASslow1 @10:17:38.10 AS.seek 0.51ml @10:17:40.40 delay 2 seconds @10:17:42.41 ATV.dial :bleach @10:17:42.89 AS.seek 0.81ml @10:18:43.54 delay 3 seconds @10:18:46.56 ATV.dial :air3 @10:18:47.10 delay 3 seconds @10:18:50.19 AS.seek 0.835ml @10:18:55.52 delay 3 seconds @10:18:58.54 ATV.dial :bleach @10:18:59.16 AS.seek 0.82ml @10:19:02.49 delay 3 seconds @10:19:05.51 ARV.dial :waste @10:19:06.53 delay 3 seconds @10:19:09.55 ATV.dial :ARV @10:19:10.42 AS.reconfigure ASconfig @10:19:10.49 AS.seek 0.47ml @10:19:30.92 AFV.select :carrierSolution @10:19:31.06 delay 2 seconds @10:19:33.09 AS.seek 1ml @10:20:03.85 delay 1 second @10:20:04.86 AFV.select :holdingCoil @10:20:05.00 delay 1 second @10:20:06.03 AS.reconfigure ASfast @10:20:06.10 AS.seek 0ml @10:20:33.45 AFV.select :carrierSolution @10:20:33.59 delay 2 seconds @10:20:35.62 AS.reconfigure ASconfig @10:20:35.69 AS.seek 1ml @10:21:33.49 delay 1 second @10:21:34.50 AFV.select :holdingCoil @10:21:34.63 delay 1 second @10:21:35.66 AS.reconfigure ASfast @10:21:35.73 AS.seek 0ml @10:22:03.06 AFV.select :carrierSolution @10:22:03.20 delay 2 seconds @10:22:05.23 AS.reconfigure ASconfig @10:22:05.30 AS.seek 1ml @10:23:03.10 delay 1 second @10:23:04.11 AFV.select :holdingCoil @10:23:04.24 delay 1 second @10:23:05.27 AS.reconfigure ASfast @10:23:05.34 AS.seek 0ml @10:23:32.70 ARV.dial between carrier and air1 @10:23:33.20 ATV.dial :air3 @10:23:34.41 AS.reconfigure ASslow1 @10:23:34.49 AS.seek 0.01ml @10:23:36.85 delay 2 seconds @10:23:38.86 ATV.dial :bleach @10:23:39.49 AS.seek 0.81ml @10:26:20.71 delay 3 seconds @10:26:23.73 ATV.dial :air3 @10:26:24.28 delay 3 seconds @10:26:27.37 AS.seek 0.835ml @10:26:32.66 delay 3 seconds @10:26:35.68 ATV.dial :bleach @10:26:36.30 AS.seek 0.82ml @10:26:39.67 delay 3 seconds @10:26:42.69 ATV.dial PCR @10:26:43.11 AS.reconfigure ASconfig @10:26:43.18 AS.seek :empty @10:27:30.67 PCR.program :bleachProfile @10:27:30.75 PCR program should complete in < 2:45 @10:27:30.77 PCR.run 10:00 @10:31:02.93 PCR.off @10:31:02.97 AFV.select :carrierSolution @10:31:03.11 delay 2 seconds @10:31:05.14 AS.seek 1ml @10:32:02.98 delay 1 second @10:32:03.99 AFV.select :holdingCoil @10:32:04.70 delay 1 second @10:32:05.73 AS.reconfigure ASfast @10:32:05.80 AS.seek 0ml @10:32:33.19 delay 3 seconds @10:32:36.21 AFV.select :carrierSolution @10:32:36.35 delay 2 seconds @10:32:38.38 AS.reconfigure ASconfig @10:32:38.46 AS.seek 1ml @10:33:36.26 delay 1 second @10:33:37.28 AFV.select :holdingCoil @10:33:37.41 delay 1 second @10:33:38.44 AS.reconfigure ASfast @10:33:38.51 AS.seek 0ml @10:34:05.87 delay 3 seconds @10:34:08.89 AFV.select :carrierSolution @10:34:09.03 delay 2 seconds @10:34:11.06 AS.reconfigure ASconfig @10:34:11.14 AS.seek 1ml @10:35:08.97 delay 1 second @10:35:09.99 AFV.select :holdingCoil @10:35:10.12 delay 1 second @10:35:11.15 AS.reconfigure ASfast @10:35:11.22 AS.seek 0ml @10:35:38.58 delay 3 seconds @10:35:41.60 AFV.select :carrierSolution @10:35:41.74 delay 2 seconds @10:35:43.77 AS.reconfigure ASconfig @10:35:43.85 AS.seek 1ml @10:36:41.69 delay 1 second @10:36:42.70 AFV.select :holdingCoil @10:36:42.84 delay 1 second @10:36:43.87 AS.reconfigure ASfast @10:36:43.95 AS.seek 0ml @10:37:11.33 delay 3 seconds @10:37:14.35 AFV.select :carrierSolution @10:37:14.49 delay 2 seconds @10:37:16.52 AS.reconfigure ASconfig @10:37:16.59 AS.seek 1ml @10:38:14.36 delay 1 second @10:38:15.37 AFV.select :holdingCoil @10:38:15.50 delay 1 second @10:38:16.53 AS.reconfigure ASnorm @10:38:16.60 AS.seek 0ml @10:40:06.53 -> exit @10:40:06.55 Session Ended @11:29:46.22 Session Begun @11:31:02.48 -> showlog 100 @11:32:53.52 -> abort! "expecting samples are inhibited, retrying with dilution.BKHA" @11:32:53.55 CmdServer::Client::Error in bkha -- Aborting Mission: expecting samples are inhibited, retrying with dilution.BKHA @11:32:53.62 BadNews.email "expecting samples are inhibited, retrying with dilution.BKHA",Subject: "18tundeployv2 ABORTED!" @11:33:03.21 Session Ended @11:36:06.10CEST04-Oct-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @11:36:08.22 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @11:36:08.52 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @11:36:13.63 Polling Can every 10:00 @11:36:15.48 SPE.coast @11:36:15.54 SPE.configure SPEconfig @11:36:15.64 AS.reconfigure ASconfig @11:36:15.69 AS.hold @11:36:15.74 AS.configure ASconfig @11:36:15.84 SS.reconfigure SSconfig @11:36:15.89 SS.hold @11:36:15.95 SS.configure SSconfig @11:36:16.05 CC.reconfigure CCconfig @11:36:16.10 CC.hold @11:36:16.15 CC.configure CCconfig @11:36:16.25 CS.reconfigure CSconfig @11:36:16.29 CS.hold @11:36:16.37 CS.configure CSconfig @11:36:16.85 PC.reconfigure PCconfig @11:36:16.90 PC.hold @11:36:16.96 PC.configure PCconfig @11:36:17.06 PS.configure PSconfig @11:36:17.18 Forearm.reconfigure LooseForearm @11:36:17.23 Forearm.hold @11:36:17.29 Forearm.configure LooseForearm @11:36:17.39 Elbow.reconfigure LooseElbow @11:36:17.44 Elbow.hold @11:36:17.55 Elbow.configure LooseElbow @11:36:17.79 SC.reconfigure SCloose @11:36:17.88 SC.hold @11:36:17.99 SC.configure SCloose @11:36:18.19 SE.reconfigure SEconfig @11:36:18.28 SE.hold @11:36:18.39 SE.configure SEconfig @11:36:18.46 CH.coast @11:36:18.51 CH.configure CHconfig @11:36:18.55 PH.coast @11:36:18.61 PH.configure PHconfig @11:36:18.66 PCR.off @11:36:18.69 PCR.pidtemp= 50,0.1,0,300,25,25 @11:36:18.72 PCR.caltemp= 2.7073,0.0373,0 @11:36:18.74 All dwarf controllers running firmware version 3.75 @11:36:18.89 Cleanly exited at 11:33:03CEST04-Oct-18 @11:36:18.93 Loading /home/esp/esp2/mission/18tundeployv2dil.rb mission @11:36:19.01 Preparing to sleep until 14:00 04-oct-18 @11:36:19.02 GoodNews.email "Starting 18tundeployv2dil w/pucks in tube 2\n{1 => 12, 2 => 10}\nCan@11:36:13, 14.7C, 67% humidity, 14.3psia, 14.001V, 0.777A, 0.777A avg, 10.88W",Subject: "18tundeployv2dil begun" @11:36:19.12 AS.coast @11:36:19.14
Asleep til 14:00 04-oct-18 @11:36:19.19 SS.coast @11:36:19.25
Continuing at 14:00 @11:36:19.30 CC.coast @11:36:19.39 CS.coast @11:36:19.43 PC.coast @11:36:19.47 Forearm.coast @11:36:19.50 Elbow.coast @11:36:19.54 SC.coast @11:36:19.58 SE.coast @11:36:19.98 Gate.power :core,:OFF @11:36:19.99 Gate.power :raw,:OFF @11:36:20.00 Shutdown @11:36:21.81 Session Begun @11:36:26.20 GoodNews.email "Then, in tube 2: phasewithdil\n/home/esp/esp2/mission/18tundeployv2dil.rb:113\n{1 => 12, 2 => 10}\nCan@11:36:19, 14.6C, 67% humidity, 14.3psia, 14.001V, 0.771A, 0.771A avg, 10.79W",Subject: "Asleep until 14:00" @11:36:57.92 -> exit @11:36:57.94 Session Ended @14:00:00.06
Powering core bus @14:00:04.72 Gate.power :raw,:ON @14:00:05.44 SPE.coast @14:00:05.51 SPE.configure SPEconfig @14:00:05.68 AS.configure ASconfig @14:00:05.78 SS.configure SSconfig @14:00:05.89 CC.configure CCconfig @14:00:05.99 CS.configure CSconfig @14:00:06.10 PC.configure PCconfig @14:00:06.19 PS.configure PSconfig @14:00:06.32 Forearm.configure LooseForearm @14:00:06.41 Elbow.configure LooseElbow @14:00:06.54 SC.configure SCloose @14:00:06.64 SE.configure SEconfig @14:00:06.69 CH.coast @14:00:06.75 CH.configure CHconfig @14:00:06.78 PH.coast @14:00:06.85 PH.configure PHconfig @14:00:06.89 PCR.off @14:00:06.92 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:06.94 PCR.caltemp= 2.7073,0.0373,0 @14:00:06.97 All dwarf controllers running firmware version 3.75 @14:00:07.09 Valve.reset @14:00:07.26 PCR.off @14:00:07.29 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:07.32 PCR.caltemp= 2.7073,0.0373,0 @14:00:07.56 Intake.select :close @14:00:07.60 PSV.dial :air @14:00:07.63 Exhaust.select :close @14:00:07.65 CTV.dial :air @14:00:08.20 CSV.dial :waste1 @14:00:08.26 PS.reconfigure PSconfig @14:00:08.37 PS.home.seek 0ml @14:00:08.45 SSV.dial :spit,avoiding: :exhaust @14:00:08.48
Forearm.reconfigure LooseForearm @14:00:08.59 Forearm.home.seek :retract @14:00:08.75 CS.reconfigure CSfast @14:00:08.79 SS.reconfigure SSconfig @14:00:08.89 CS.home.seek 0ml @14:00:08.92 SS.home.seek 0ml @14:00:09.39 Hand.close @14:00:09.44
Elbow.jog 1500 @14:00:09.99 Elbow.reconfigure LooseElbow @14:00:10.04 Elbow.home.seek :clear @14:00:14.71 SE.reconfigure SEconfig @14:00:14.76 SE.home.seek :down @14:00:15.50 SC.reconfigure SCsloppy @14:00:15.55 SC.home.seek :initial @14:00:17.45 PS.coast @14:00:19.50
Forearm.seek :retract @14:00:19.58 Elbow.seek :hammer @14:00:20.79 Forearm.reconfigure TightForearm @14:00:20.84 Forearm.seek 5 + 350 counts @14:00:21.87 Forearm.seek :retract @14:00:23.02 PC.reconfigure PCconfig @14:00:23.03
CC.reconfigure CCconfig @14:00:23.12 CC.home.seek :open @14:00:23.16 PC.home.seek :open @14:00:26.48
Phase [phasewithdil] @14:00:26.50 GoodNews.email "At 14:00:26 began in tube 2: phasewithdil\n/home/esp/esp2/mission/18tundeployv2dil.rb:113\n{1 => 12, 2 => 10}\nCan@14:00:26, 14.2C, 68% humidity, 14.3psia, 13.996V, 0.998A, 0.998A avg, 13.96W",Subject: "Started 18tundeployv2dil phase phasewithdil" @14:00:26.51CEST04-Oct-18
==================> STARTED Superphasewdil (phase #1) <=== @14:00:26.55 Starting initialPurge @14:00:26.56 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:00:26.64 Hand.open @14:00:27.37 Elbow.reconfigure TightElbow @14:00:27.42 Elbow.seek FlushPuck::Garage @14:00:29.32 Elbow.reconfigure LooseElbow @14:00:29.43 Forearm.reconfigure LooseForearm @14:00:29.48 Forearm.seek :garage @14:00:30.47 Hand.close @14:00:31.23 Forearm.seek :retract @14:00:32.21 Elbow.reconfigure TightElbow @14:00:32.27 Elbow.seek CC @14:00:35.22 Elbow.reconfigure LooseElbow @14:00:35.34 Forearm.reconfigure TightForearm @14:00:35.39 Forearm.seek CC @14:00:36.63 CC.seek :closed @14:00:40.90 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.79 @14:00:40.98 Hand.open @14:00:41.66 Forearm.reconfigure LooseForearm @14:00:41.71 Forearm.seek :retract @14:00:42.67 Flushing the Collection Valve::Manifold @14:00:42.80 CSV.dial CSR @14:00:44.14 Pulling 5ml of CSR.flush @14:00:44.15 CSR.select :flush @14:00:44.27 CS.reconfigure CSconfig @14:00:44.31 CS.seek 5ml @14:00:58.00 delay 5 seconds @14:01:03.02 CSR.select :air @14:01:03.19 CS.reconfigure CSfast @14:01:03.24 CS.seek 7.5ml @14:01:09.84 CSV.dial :waste @14:01:11.15 CS.reconfigure CSconfig @14:01:11.20 CS.seek 0ml @14:01:31.41 Purging external Intake @14:01:31.54 CSV.dial :flush @14:01:31.99 CS.reconfigure CSfast @14:01:32.04 CS.seek 10ml @14:01:55.07 delay 5 seconds @14:02:00.10 CSV.dial CBV @14:02:00.14
CBV.dial CSV @14:02:00.90 CTV.dial :intake @14:02:01.64 Intake.select :open @14:02:01.77 CS.seek 0ml @14:02:25.46 Intake.select :close @14:02:25.58 CTV.dial :air @14:02:26.33 Flushing upper manifold @14:02:26.47 CSV.dial :flush @14:02:27.22 CS.seek 5ml @14:02:39.26 delay 5 seconds @14:02:44.28 CSV.dial :air @14:02:44.81 CS.seek 7ml @14:02:50.29 CSV.dial CBV @14:02:50.31
CBV.dial CSV @14:02:51.35 CTV.dial :air @14:02:51.37 CS.seek 0ml @14:03:07.69 delay 7 seconds @14:03:14.72 CS.seek 3ml @14:03:22.34 CSV.dial :waste @14:03:22.81 CS.seek 0ml @14:03:30.91 CSV.dial :flush @14:03:31.38 CS.seek 3ml @14:03:39.48 delay 5 seconds @14:03:44.50 CSV.dial :air @14:03:45.03 CS.seek 5ml @14:03:50.56 CSV.dial :air @14:03:50.59 CS.seek 0ml @14:04:02.57 delay 7 seconds @14:04:09.61 CS.seek 3ml @14:04:17.19 CSV.dial :waste @14:04:17.96 CS.seek 0ml @14:04:25.57 Garaging FlushPuck in Collection Clamp @14:04:25.65 Elbow.reconfigure TightElbow @14:04:25.70 Elbow.seek CC @14:04:25.94 Elbow.reconfigure LooseElbow @14:04:26.05 Forearm.seek CC @14:04:27.03 Hand.close @14:04:27.71 CC.seek :open @14:04:32.08 Forearm.seek :retract @14:04:33.04 Elbow.reconfigure TightElbow @14:04:33.09 Elbow.seek :garage @14:04:36.21 Elbow.reconfigure LooseElbow @14:04:36.32 Forearm.reconfigure TightForearm @14:04:36.37 Forearm.seek :garage @14:04:37.53 Hand.open @14:04:38.28 Forearm.reconfigure LooseForearm @14:04:38.33 Forearm.seek :retract @14:04:39.45 Starting mfb @14:04:39.47 pcrBAC sampling at most 1500ml, wcr at most 1500ml @14:04:39.51 BACfans.email "Started mfb in tube 2 at 14:04:39CEST04-Oct-18\n{1 => 12, 2 => 10}\nCan@14:00:26, 14.2C, 68% humidity, 14.3psia, 13.996V, 0.998A, 0.998A avg, 13.96W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @14:04:39.57
Loading sh1 puck from tube 2 into Collection Clamp @14:04:39.70 Readying the MFB @14:04:39.76 Homing MFB Syringe @14:04:39.77 AFV.select :holdingCoil @14:04:40.02 ATV.dial :air2 @14:04:40.18
SC.reconfigure SCloose @14:04:40.43 Elbow.reconfigure TightElbow @14:04:40.48 Elbow.seek 2 @14:04:41.45 AS.reconfigure ASconfig @14:04:41.50 AS.home.seek :home @14:04:42.53
Forearm.reconfigure TightForearm @14:04:42.59 Forearm.seek 2 + 700 counts @14:04:43.60 SE.reconfigure SEpick @14:04:43.70 SE.seek beamBreak @14:04:45.85 SE.configure SEconfig @14:04:46.05 Elbow.seek 2 @14:04:46.26 Forearm.seek 2 + 300 counts @14:04:46.52 Forearm.reconfigure LooseForearm @14:04:46.57 Hand.close @14:04:47.03 AFV.select :carrierSolution @14:04:47.15 delay 2 seconds @14:04:47.23 SE.seek :down @14:04:47.48
Forearm.seek :retract @14:04:48.46 Elbow.seek CC @14:04:49.19 AS.seek 1ml @14:04:49.26 SE.coast @14:04:50.52
Elbow.reconfigure LooseElbow @14:04:50.71 Forearm.reconfigure TightForearm @14:04:50.76 Forearm.seek CC @14:04:52.08 CC.seek :closed @14:04:56.35 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.87 @14:04:56.36 Hand.open @14:04:57.06 Forearm.reconfigure LooseForearm @14:04:57.11 Forearm.seek :retract @14:04:58.07 SC.coast @14:04:58.11 collectSample 1500ml w/bubble point of 55psi @14:04:58.12 Priming 7ml @14:04:58.13 Intake.select :close @14:04:58.14 Exhaust.select :close @14:04:58.16 SS.hold @14:04:58.40 Calibrating pressure and temperature sensors @14:04:58.42 CTV.dial :air @14:04:58.43 SSV.dial :spit,avoiding: :exhaust @14:04:58.45 SS.reconfigure SSfast @14:04:58.52 SS.seek 5ml @14:05:01.22 SSV.dial CBV @14:05:01.25
CBV.dial SSV @14:05:02.63 delay 3.8 seconds @14:05:06.62 SSV.dial :spit @14:05:07.97 SS.seek 0ml @14:05:10.53 SSV.dial CBV @14:05:11.87 CTV.dial :intake @14:05:12.63 SSV.dial CBV @14:05:12.66
CBV.dial SSV @14:05:12.67 Allowing 40s for 7.0ml of remaining 7.0ml @14:05:12.68 Intake.select :open @14:05:14.86 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:05:14.88 SS.reconfigure SSinhale.with maxSpeed: 150 @14:05:14.93 SS.seek 7ml @14:05:18.03 Vacuum too high -- waiting up to 40s @14:05:19.67 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:05:19.69 SS.reconfigure SSinhale.with maxSpeed: 135 @14:05:19.74 SS.seek 7ml @14:05:22.58 Vacuum too high -- waiting up to 40s @14:05:24.22 SS.reconfigure SSinhale.with maxSpeed: 122 @14:05:24.27 SS.seek 7ml @14:05:26.45 Stroke completed -- waiting for pressure to equalize ... @14:05:28.43 Intake.select :close @14:05:28.59 SSV.dial :exhaust @14:05:29.36 Exhaust.select :open @14:05:29.47 SS.reconfigure SSexhale @14:05:29.52 SS.seek 0ml @14:05:33.20 Exhaust.select :close @14:05:33.32 Sampling 1500.0ml @14:05:33.35 Intake.select :close @14:05:33.36 Exhaust.select :close @14:05:33.37 SS.reconfigure SSconfig @14:05:33.42 SS.hold @14:05:33.64 CTV.dial :intake @14:05:33.66 SSV.dial CBV @14:05:33.68
CBV.dial SSV @14:05:34.47 Allowing 130s for 25.0ml of remaining 1500.0ml @14:05:34.48 Intake.select :open @14:05:36.65 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:05:36.67 SS.reconfigure SSinhale.with maxSpeed: 122 @14:05:36.72 SS.seek 25ml @14:05:40.17 Vacuum too high -- waiting up to 40s @14:05:41.54 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:05:41.56 SS.reconfigure SSinhale.with maxSpeed: 110 @14:05:41.61 SS.seek 25ml @14:05:42.10 delay 1 second @14:05:43.11 AFV.select :holdingCoil @14:05:43.22 delay 1 second @14:05:44.03
Vacuum too high -- waiting up to 40s @14:05:44.31 ATV.dial :waste @14:05:45.39 AS.reconfigure ASnorm @14:05:45.47 AS.home.seek :empty @14:05:45.54
Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:05:45.56 SS.reconfigure SSinhale.with maxSpeed: 99 @14:05:45.61 SS.seek 25ml @14:05:48.33 Vacuum too high -- waiting up to 40s @14:05:49.73 SS.reconfigure SSinhale.with maxSpeed: 89 @14:05:49.78 SS.seek 25ml @14:05:52.68 Vacuum too high -- waiting up to 40s @14:05:54.01 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:05:54.03 SS.reconfigure SSinhale.with maxSpeed: 80 @14:05:54.08 SS.seek 25ml @14:05:57.23 Vacuum too high -- waiting up to 40s @14:05:58.33 SS.reconfigure SSinhale.with maxSpeed: 72 @14:05:58.38 SS.seek 25ml @14:06:01.48 Vacuum too high -- waiting up to 40s @14:06:02.59 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:06:02.61 SS.reconfigure SSinhale.with maxSpeed: 65 @14:06:02.66 SS.seek 25ml @14:06:06.34 Vacuum too high -- waiting up to 40s @14:06:07.43 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:06:07.45 SS.reconfigure SSinhale.with maxSpeed: 59 @14:06:07.50 SS.seek 25ml @14:06:13.15 Vacuum too high -- waiting up to 40s @14:06:14.25 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:06:14.27 SS.reconfigure SSinhale.with maxSpeed: 53 @14:06:14.31 SS.seek 25ml @14:06:16.49 Stroke completed -- waiting for pressure to equalize ... @14:06:19.11 Intake.select :close @14:06:19.27 SSV.dial :exhaust @14:06:20.07 Exhaust.select :open @14:06:20.18 SS.reconfigure SSexhale @14:06:20.23 SS.seek 0ml @14:06:28.93 Exhaust.select :close @14:06:29.05 SSV.dial CBV @14:06:29.08
CBV.dial SSV @14:06:29.88 Allowing 130s for 25.0ml of remaining 1475.0ml @14:06:29.89 Intake.select :open @14:06:32.07 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:06:32.08 SS.reconfigure SSinhale.with maxSpeed: 53 @14:06:32.13 SS.seek 25ml @14:06:38.55 ATV.dial :waste @14:06:38.57 ARV.dial between carrier and air1 @14:06:38.66 ASV.dial :waste1 @14:06:42.11
Vacuum too high -- waiting up to 40s @14:06:42.96 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:06:42.98 SS.reconfigure SSinhale.with maxSpeed: 48 @14:06:43.03 SS.seek 25ml @14:07:10.03 Stroke completed -- waiting for pressure to equalize ... @14:07:12.63 Intake.select :close @14:07:12.79 SSV.dial :exhaust @14:07:13.58 Exhaust.select :open @14:07:13.69 SS.reconfigure SSexhale @14:07:13.74 SS.seek 0ml @14:07:22.39 Exhaust.select :close @14:07:22.51 SSV.dial CBV @14:07:22.54
CBV.dial SSV @14:07:23.33 Allowing 130s for 25.0ml of remaining 1450.0ml @14:07:23.34 Intake.select :open @14:07:25.51 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:07:25.53 SS.reconfigure SSinhale.with maxSpeed: 48 @14:07:25.58 SS.seek 25ml @14:08:03.09 Stroke completed -- waiting for pressure to equalize ... @14:08:05.69 Intake.select :close @14:08:05.85 SSV.dial :exhaust @14:08:06.63 Exhaust.select :open @14:08:06.74 SS.reconfigure SSexhale @14:08:06.79 SS.seek 0ml @14:08:15.57 Exhaust.select :close @14:08:15.69 SSV.dial CBV @14:08:15.72
CBV.dial SSV @14:08:16.50 Allowing 130s for 25.0ml of remaining 1425.0ml @14:08:16.51 Intake.select :open @14:08:18.69 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:08:18.70 SS.reconfigure SSinhale.with maxSpeed: 48 @14:08:18.75 SS.seek 25ml @14:08:56.26 Stroke completed -- waiting for pressure to equalize ... @14:08:58.88 Intake.select :close @14:08:59.04 SSV.dial :exhaust @14:08:59.82 Exhaust.select :open @14:08:59.93 SS.reconfigure SSexhale @14:08:59.98 SS.seek 0ml @14:09:08.87 Exhaust.select :close @14:09:08.99 SSV.dial CBV @14:09:09.02
CBV.dial SSV @14:09:09.82 Allowing 130s for 25.0ml of remaining 1400.0ml @14:09:09.83 Intake.select :open @14:09:12.01 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:09:12.02 SS.reconfigure SSinhale.with maxSpeed: 48 @14:09:12.08 SS.seek 25ml @14:09:49.24 Stroke completed -- waiting for pressure to equalize ... @14:09:52.42 Intake.select :close @14:09:52.58 SSV.dial :exhaust @14:09:53.36 Exhaust.select :open @14:09:53.47 SS.reconfigure SSexhale @14:09:53.53 SS.seek 0ml @14:10:02.23 Exhaust.select :close @14:10:02.35 SSV.dial CBV @14:10:02.38
CBV.dial SSV @14:10:03.16 Allowing 130s for 25.0ml of remaining 1375.0ml @14:10:03.17 Intake.select :open @14:10:05.34 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:10:05.36 SS.reconfigure SSinhale.with maxSpeed: 48 @14:10:05.41 SS.seek 25ml @14:10:42.95 Stroke completed -- waiting for pressure to equalize ... @14:10:46.18 Intake.select :close @14:10:46.34 SSV.dial :exhaust @14:10:47.11 Exhaust.select :open @14:10:47.22 SS.reconfigure SSexhale @14:10:47.28 SS.seek 0ml @14:10:56.53 Exhaust.select :close @14:10:56.65 SSV.dial CBV @14:10:56.68
CBV.dial SSV @14:10:57.49 Allowing 130s for 25.0ml of remaining 1350.0ml @14:10:57.50 Intake.select :open @14:10:59.68 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:10:59.69 SS.reconfigure SSinhale.with maxSpeed: 48 @14:10:59.75 SS.seek 25ml @14:11:28.42 Vacuum too high -- waiting up to 40s @14:11:29.27 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:11:29.29 SS.reconfigure SSinhale.with maxSpeed: 43 @14:11:29.34 SS.seek 25ml @14:11:39.28 Stroke completed -- waiting for pressure to equalize ... @14:11:41.88 Intake.select :close @14:11:42.04 SSV.dial :exhaust @14:11:42.82 Exhaust.select :open @14:11:42.93 SS.reconfigure SSexhale @14:11:42.98 SS.seek 0ml @14:11:52.15 Exhaust.select :close @14:11:52.27 SSV.dial CBV @14:11:52.30
CBV.dial SSV @14:11:53.10 Allowing 130s for 25.0ml of remaining 1325.0ml @14:11:53.11 Intake.select :open @14:11:55.28 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:11:55.30 SS.reconfigure SSinhale.with maxSpeed: 43 @14:11:55.35 SS.seek 25ml @14:12:36.17 Stroke completed -- waiting for pressure to equalize ... @14:12:38.78 Intake.select :close @14:12:38.94 SSV.dial :exhaust @14:12:39.74 Exhaust.select :open @14:12:39.85 SS.reconfigure SSexhale @14:12:39.90 SS.seek 0ml @14:12:48.92 Exhaust.select :close @14:12:49.04 SSV.dial CBV @14:12:49.07
CBV.dial SSV @14:12:49.86 Allowing 130s for 25.0ml of remaining 1300.0ml @14:12:49.87 Intake.select :open @14:12:52.04 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:12:52.06 SS.reconfigure SSinhale.with maxSpeed: 43 @14:12:52.11 SS.seek 25ml @14:13:33.26 Stroke completed -- waiting for pressure to equalize ... @14:13:35.85 Intake.select :close @14:13:36.01 SSV.dial :exhaust @14:13:36.79 Exhaust.select :open @14:13:36.90 SS.reconfigure SSexhale @14:13:36.95 SS.seek 0ml @14:13:45.82 Exhaust.select :close @14:13:45.94 SSV.dial CBV @14:13:45.97
CBV.dial SSV @14:13:46.77 Allowing 130s for 25.0ml of remaining 1275.0ml @14:13:46.78 Intake.select :open @14:13:48.95 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:13:48.97 SS.reconfigure SSinhale.with maxSpeed: 43 @14:13:49.02 SS.seek 25ml @14:14:30.70 Stroke completed -- waiting for pressure to equalize ... @14:14:33.28 Intake.select :close @14:14:33.43 SSV.dial :exhaust @14:14:34.21 Exhaust.select :open @14:14:34.33 SS.reconfigure SSexhale @14:14:34.38 SS.seek 0ml @14:14:43.49 Exhaust.select :close @14:14:43.61 SSV.dial CBV @14:14:43.64
CBV.dial SSV @14:14:44.43 Allowing 130s for 25.0ml of remaining 1250.0ml @14:14:44.44 Intake.select :open @14:14:46.61 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:14:46.63 SS.reconfigure SSinhale.with maxSpeed: 43 @14:14:46.68 SS.seek 25ml @14:15:27.98 Stroke completed -- waiting for pressure to equalize ... @14:15:30.58 Intake.select :close @14:15:30.74 SSV.dial :exhaust @14:15:31.52 Exhaust.select :open @14:15:31.63 SS.reconfigure SSexhale @14:15:31.68 SS.seek 0ml @14:15:40.44 Exhaust.select :close @14:15:40.56 SSV.dial CBV @14:15:40.61
CBV.dial SSV @14:15:41.38 Allowing 130s for 25.0ml of remaining 1225.0ml @14:15:41.39 Intake.select :open @14:15:43.57 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:15:43.58 SS.reconfigure SSinhale.with maxSpeed: 43 @14:15:43.64 SS.seek 25ml @14:16:25.01 Stroke completed -- waiting for pressure to equalize ... @14:16:27.62 Intake.select :close @14:16:27.78 SSV.dial :exhaust @14:16:28.55 Exhaust.select :open @14:16:28.66 SS.reconfigure SSexhale @14:16:28.71 SS.seek 0ml @14:16:37.42 Exhaust.select :close @14:16:37.54 SSV.dial CBV @14:16:37.57
CBV.dial SSV @14:16:38.38 Allowing 130s for 25.0ml of remaining 1200.0ml @14:16:38.39 Intake.select :open @14:16:40.57 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:16:40.58 SS.reconfigure SSinhale.with maxSpeed: 43 @14:16:40.63 SS.seek 25ml @14:17:22.24 Stroke completed -- waiting for pressure to equalize ... @14:17:24.85 Intake.select :close @14:17:25.01 SSV.dial :exhaust @14:17:25.80 Exhaust.select :open @14:17:25.91 SS.reconfigure SSexhale @14:17:25.96 SS.seek 0ml @14:17:34.76 Exhaust.select :close @14:17:34.88 SSV.dial CBV @14:17:34.91
CBV.dial SSV @14:17:35.71 Allowing 130s for 25.0ml of remaining 1175.0ml @14:17:35.72 Intake.select :open @14:17:37.89 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:17:37.91 SS.reconfigure SSinhale.with maxSpeed: 43 @14:17:37.96 SS.seek 25ml @14:18:19.62 Stroke completed -- waiting for pressure to equalize ... @14:18:22.85 Intake.select :close @14:18:23.01 SSV.dial :exhaust @14:18:23.80 Exhaust.select :open @14:18:23.91 SS.reconfigure SSexhale @14:18:23.96 SS.seek 0ml @14:18:32.92 Exhaust.select :close @14:18:33.04 SSV.dial CBV @14:18:33.07
CBV.dial SSV @14:18:33.86 Allowing 130s for 25.0ml of remaining 1150.0ml @14:18:33.87 Intake.select :open @14:18:36.04 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:18:36.06 SS.reconfigure SSinhale.with maxSpeed: 43 @14:18:36.11 SS.seek 25ml @14:19:17.38 Stroke completed -- waiting for pressure to equalize ... @14:19:20.62 Intake.select :close @14:19:20.78 SSV.dial :exhaust @14:19:21.57 Exhaust.select :open @14:19:21.68 SS.reconfigure SSexhale @14:19:21.73 SS.seek 0ml @14:19:30.71 Exhaust.select :close @14:19:30.83 SSV.dial CBV @14:19:30.94
CBV.dial SSV @14:19:31.68 Allowing 130s for 25.0ml of remaining 1125.0ml @14:19:31.69 Intake.select :open @14:19:33.86 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:19:33.88 SS.reconfigure SSinhale.with maxSpeed: 43 @14:19:33.93 SS.seek 25ml @14:20:15.29 Stroke completed -- waiting for pressure to equalize ... @14:20:18.36 Intake.select :close @14:20:18.52 SSV.dial :exhaust @14:20:19.30 Exhaust.select :open @14:20:19.41 SS.reconfigure SSexhale @14:20:19.46 SS.seek 0ml @14:20:28.48 Exhaust.select :close @14:20:28.60 SSV.dial CBV @14:20:28.63
CBV.dial SSV @14:20:29.44 Allowing 130s for 25.0ml of remaining 1100.0ml @14:20:29.45 Intake.select :open @14:20:31.64 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:20:31.66 SS.reconfigure SSinhale.with maxSpeed: 43 @14:20:31.71 SS.seek 25ml @14:21:12.99 Stroke completed -- waiting for pressure to equalize ... @14:21:16.23 Intake.select :close @14:21:16.39 SSV.dial :exhaust @14:21:17.18 Exhaust.select :open @14:21:17.29 SS.reconfigure SSexhale @14:21:17.34 SS.seek 0ml @14:21:26.52 Exhaust.select :close @14:21:26.64 SSV.dial CBV @14:21:26.67
CBV.dial SSV @14:21:27.48 Allowing 130s for 25.0ml of remaining 1075.0ml @14:21:27.51 Intake.select :open @14:21:29.73 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:21:29.74 SS.reconfigure SSinhale.with maxSpeed: 43 @14:21:29.80 SS.seek 25ml @14:21:59.92 Vacuum too high -- waiting up to 40s @14:22:00.76 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:22:00.78 SS.reconfigure SSinhale.with maxSpeed: 39 @14:22:00.83 SS.seek 25ml @14:22:12.87 Stroke completed -- waiting for pressure to equalize ... @14:22:15.46 Intake.select :close @14:22:15.62 SSV.dial :exhaust @14:22:16.39 Exhaust.select :open @14:22:16.50 SS.reconfigure SSexhale @14:22:16.55 SS.seek 0ml @14:22:25.32 Exhaust.select :close @14:22:25.44 SSV.dial CBV @14:22:25.47
CBV.dial SSV @14:22:26.27 Allowing 130s for 25.0ml of remaining 1050.0ml @14:22:26.28 Intake.select :open @14:22:28.46 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:22:28.48 SS.reconfigure SSinhale.with maxSpeed: 39 @14:22:28.53 SS.seek 25ml @14:23:14.23 Stroke completed -- waiting for pressure to equalize ... @14:23:17.46 Intake.select :close @14:23:17.62 SSV.dial :exhaust @14:23:18.39 Exhaust.select :open @14:23:18.50 SS.reconfigure SSexhale @14:23:18.55 SS.seek 0ml @14:23:27.48 Exhaust.select :close @14:23:27.60 SSV.dial CBV @14:23:27.63
CBV.dial SSV @14:23:28.44 Allowing 130s for 25.0ml of remaining 1025.0ml @14:23:28.45 Intake.select :open @14:23:30.63 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:23:30.64 SS.reconfigure SSinhale.with maxSpeed: 39 @14:23:30.69 SS.seek 25ml @14:24:16.04 Stroke completed -- waiting for pressure to equalize ... @14:24:19.12 Intake.select :close @14:24:19.28 SSV.dial :exhaust @14:24:20.08 Exhaust.select :open @14:24:20.19 SS.reconfigure SSexhale @14:24:20.24 SS.seek 0ml @14:24:29.06 Exhaust.select :close @14:24:29.18 SSV.dial CBV @14:24:29.21
CBV.dial SSV @14:24:30.02 Allowing 130s for 25.0ml of remaining 1000.0ml @14:24:30.03 Intake.select :open @14:24:32.22 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:24:32.23 SS.reconfigure SSinhale.with maxSpeed: 39 @14:24:32.28 SS.seek 25ml @14:25:18.01 Stroke completed -- waiting for pressure to equalize ... @14:25:21.27 Intake.select :close @14:25:21.43 SSV.dial :exhaust @14:25:22.22 Exhaust.select :open @14:25:22.33 SS.reconfigure SSexhale @14:25:22.38 SS.seek 0ml @14:25:31.15 Exhaust.select :close @14:25:31.27 SSV.dial CBV @14:25:31.30
CBV.dial SSV @14:25:32.08 Allowing 130s for 25.0ml of remaining 975.0ml @14:25:32.09 Intake.select :open @14:25:34.26 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:25:34.28 SS.reconfigure SSinhale.with maxSpeed: 39 @14:25:34.33 SS.seek 25ml @14:26:20.06 Stroke completed -- waiting for pressure to equalize ... @14:26:23.29 Intake.select :close @14:26:23.45 SSV.dial :exhaust @14:26:24.24 Exhaust.select :open @14:26:24.35 SS.reconfigure SSexhale @14:26:24.40 SS.seek 0ml @14:26:33.17 Exhaust.select :close @14:26:33.29 SSV.dial CBV @14:26:33.32
CBV.dial SSV @14:26:34.13 Allowing 130s for 25.0ml of remaining 950.0ml @14:26:34.14 Intake.select :open @14:26:36.31 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:26:36.33 SS.reconfigure SSinhale.with maxSpeed: 39 @14:26:36.38 SS.seek 25ml @14:27:22.10 Stroke completed -- waiting for pressure to equalize ... @14:27:25.33 Intake.select :close @14:27:25.49 SSV.dial :exhaust @14:27:26.28 Exhaust.select :open @14:27:26.39 SS.reconfigure SSexhale @14:27:26.45 SS.seek 0ml @14:27:35.27 Exhaust.select :close @14:27:35.39 SSV.dial CBV @14:27:35.42
CBV.dial SSV @14:27:36.21 Allowing 130s for 25.0ml of remaining 925.0ml @14:27:36.22 Intake.select :open @14:27:38.40 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:27:38.41 SS.reconfigure SSinhale.with maxSpeed: 39 @14:27:38.46 SS.seek 25ml @14:28:23.26 Vacuum too high -- waiting up to 40s @14:28:24.08 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:28:24.10 SS.reconfigure SSinhale.with maxSpeed: 35 @14:28:24.15 SS.seek 25ml @14:28:25.35 Stroke completed -- waiting for pressure to equalize ... @14:28:27.81 Intake.select :close @14:28:27.97 SSV.dial :exhaust @14:28:28.76 Exhaust.select :open @14:28:28.87 SS.reconfigure SSexhale @14:28:28.92 SS.seek 0ml @14:28:37.87 Exhaust.select :close @14:28:37.99 SSV.dial CBV @14:28:38.02
CBV.dial SSV @14:28:38.83 Allowing 130s for 25.0ml of remaining 900.0ml @14:28:38.84 Intake.select :open @14:28:41.03 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:28:41.04 SS.reconfigure SSinhale.with maxSpeed: 35 @14:28:41.10 SS.seek 25ml @14:29:31.88 Stroke completed -- waiting for pressure to equalize ... @14:29:35.10 Intake.select :close @14:29:35.26 SSV.dial :exhaust @14:29:36.05 Exhaust.select :open @14:29:36.16 SS.reconfigure SSexhale @14:29:36.21 SS.seek 0ml @14:29:45.07 Exhaust.select :close @14:29:45.19 SSV.dial CBV @14:29:45.22
CBV.dial SSV @14:29:46.02 Allowing 130s for 25.0ml of remaining 875.0ml @14:29:46.03 Intake.select :open @14:29:48.20 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:29:48.22 SS.reconfigure SSinhale.with maxSpeed: 35 @14:29:48.27 SS.seek 25ml @14:30:38.99 Stroke completed -- waiting for pressure to equalize ... @14:30:42.23 Intake.select :close @14:30:42.39 SSV.dial :exhaust @14:30:43.18 Exhaust.select :open @14:30:43.29 SS.reconfigure SSexhale @14:30:43.34 SS.seek 0ml @14:30:52.16 Exhaust.select :close @14:30:52.28 SSV.dial CBV @14:30:52.31
CBV.dial SSV @14:30:53.10 Allowing 130s for 25.0ml of remaining 850.0ml @14:30:53.11 Intake.select :open @14:30:55.29 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:30:55.30 SS.reconfigure SSinhale.with maxSpeed: 35 @14:30:55.36 SS.seek 25ml @14:31:46.06 Stroke completed -- waiting for pressure to equalize ... @14:31:49.30 Intake.select :close @14:31:49.46 SSV.dial :exhaust @14:31:50.24 Exhaust.select :open @14:31:50.35 SS.reconfigure SSexhale @14:31:50.40 SS.seek 0ml @14:31:59.23 Exhaust.select :close @14:31:59.35 SSV.dial CBV @14:31:59.38
CBV.dial SSV @14:32:00.19 Allowing 130s for 25.0ml of remaining 825.0ml @14:32:00.20 Intake.select :open @14:32:02.37 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:32:02.39 SS.reconfigure SSinhale.with maxSpeed: 35 @14:32:02.44 SS.seek 25ml @14:32:53.16 Stroke completed -- waiting for pressure to equalize ... @14:32:57.51 Intake.select :close @14:32:57.67 SSV.dial :exhaust @14:32:58.48 Exhaust.select :open @14:32:58.60 SS.reconfigure SSexhale @14:32:58.65 SS.seek 0ml @14:33:07.34 Exhaust.select :close @14:33:07.46 SSV.dial CBV @14:33:07.49
CBV.dial SSV @14:33:08.30 Allowing 130s for 25.0ml of remaining 800.0ml @14:33:08.31 Intake.select :open @14:33:10.50 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:33:10.52 SS.reconfigure SSinhale.with maxSpeed: 35 @14:33:10.57 SS.seek 25ml @14:34:01.27 Stroke completed -- waiting for pressure to equalize ... @14:34:05.16 Intake.select :close @14:34:05.32 SSV.dial :exhaust @14:34:06.11 Exhaust.select :open @14:34:06.22 SS.reconfigure SSexhale @14:34:06.27 SS.seek 0ml @14:34:15.34 Exhaust.select :close @14:34:15.46 SSV.dial CBV @14:34:15.49
CBV.dial SSV @14:34:16.28 Allowing 130s for 25.0ml of remaining 775.0ml @14:34:16.29 Intake.select :open @14:34:18.46 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:34:18.48 SS.reconfigure SSinhale.with maxSpeed: 35 @14:34:18.53 SS.seek 25ml @14:35:08.60 Stroke completed -- waiting for pressure to equalize ... @14:35:12.46 Intake.select :close @14:35:12.62 SSV.dial :exhaust @14:35:13.41 Exhaust.select :open @14:35:13.52 SS.reconfigure SSexhale @14:35:13.57 SS.seek 0ml @14:35:22.26 Exhaust.select :close @14:35:22.38 SSV.dial CBV @14:35:22.41
CBV.dial SSV @14:35:23.22 Allowing 130s for 25.0ml of remaining 750.0ml @14:35:23.23 Intake.select :open @14:35:25.42 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:35:25.43 SS.reconfigure SSinhale.with maxSpeed: 35 @14:35:25.49 SS.seek 25ml @14:36:08.26 Vacuum too high -- waiting up to 40s @14:36:09.37 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:36:09.39 SS.reconfigure SSinhale.with maxSpeed: 32 @14:36:09.44 SS.seek 25ml @14:36:18.32 Stroke completed -- waiting for pressure to equalize ... @14:36:21.58 Intake.select :close @14:36:21.75 SSV.dial :exhaust @14:36:22.55 Exhaust.select :open @14:36:22.66 SS.reconfigure SSexhale @14:36:22.72 SS.seek 0ml @14:36:31.66 Exhaust.select :close @14:36:31.78 SSV.dial CBV @14:36:31.81
CBV.dial SSV @14:36:32.60 Allowing 130s for 25.0ml of remaining 725.0ml @14:36:32.61 Intake.select :open @14:36:34.78 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:36:34.80 SS.reconfigure SSinhale.with maxSpeed: 32 @14:36:34.85 SS.seek 25ml @14:37:30.20 Stroke completed -- waiting for pressure to equalize ... @14:37:33.92 Intake.select :close @14:37:34.08 SSV.dial :exhaust @14:37:34.86 Exhaust.select :open @14:37:34.97 SS.reconfigure SSexhale @14:37:35.02 SS.seek 0ml @14:37:43.97 Exhaust.select :close @14:37:44.08 SSV.dial CBV @14:37:44.11
CBV.dial SSV @14:37:44.91 Allowing 130s for 25.0ml of remaining 700.0ml @14:37:44.92 Intake.select :open @14:37:47.10 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:37:47.12 SS.reconfigure SSinhale.with maxSpeed: 32 @14:37:47.17 SS.seek 25ml @14:38:42.04 Stroke completed -- waiting for pressure to equalize ... @14:38:45.92 Intake.select :close @14:38:46.08 SSV.dial :exhaust @14:38:46.88 Exhaust.select :open @14:38:46.99 SS.reconfigure SSexhale @14:38:47.04 SS.seek 0ml @14:38:55.98 Exhaust.select :close @14:38:56.10 SSV.dial CBV @14:38:56.13
CBV.dial SSV @14:38:56.94 Allowing 130s for 25.0ml of remaining 675.0ml @14:38:56.95 Intake.select :open @14:38:59.14 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:38:59.15 SS.reconfigure SSinhale.with maxSpeed: 32 @14:38:59.21 SS.seek 25ml @14:39:54.29 Stroke completed -- waiting for pressure to equalize ... @14:39:58.16 Intake.select :close @14:39:58.32 SSV.dial :exhaust @14:39:59.11 Exhaust.select :open @14:39:59.22 SS.reconfigure SSexhale @14:39:59.28 SS.seek 0ml @14:40:08.29 Exhaust.select :close @14:40:08.41 SSV.dial CBV @14:40:08.44
CBV.dial SSV @14:40:09.24 Allowing 130s for 25.0ml of remaining 650.0ml @14:40:09.25 Intake.select :open @14:40:11.43 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:40:11.44 SS.reconfigure SSinhale.with maxSpeed: 32 @14:40:11.50 SS.seek 25ml @14:41:03.01 Vacuum too high -- waiting up to 40s @14:41:03.87 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:41:03.89 SS.reconfigure SSinhale.with maxSpeed: 29 @14:41:03.94 SS.seek 25ml @14:41:08.42 Stroke completed -- waiting for pressure to equalize ... @14:41:11.66 Intake.select :close @14:41:11.82 SSV.dial :exhaust @14:41:12.61 Exhaust.select :open @14:41:12.72 SS.reconfigure SSexhale @14:41:12.77 SS.seek 0ml @14:41:21.84 Exhaust.select :close @14:41:21.97 SSV.dial CBV @14:41:22.52
CBV.dial SSV @14:41:23.27 Allowing 130s for 25.0ml of remaining 625.0ml @14:41:23.28 Intake.select :open @14:41:25.47 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:41:25.48 SS.reconfigure SSinhale.with maxSpeed: 29 @14:41:25.54 SS.seek 25ml @14:42:26.13 Stroke completed -- waiting for pressure to equalize ... @14:42:30.02 Intake.select :close @14:42:30.18 SSV.dial :exhaust @14:42:30.97 Exhaust.select :open @14:42:31.08 SS.reconfigure SSexhale @14:42:31.13 SS.seek 0ml @14:42:40.10 Exhaust.select :close @14:42:40.22 SSV.dial CBV @14:42:40.25
CBV.dial SSV @14:42:41.05 Allowing 130s for 25.0ml of remaining 600.0ml @14:42:41.06 Intake.select :open @14:42:43.25 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:42:43.26 SS.reconfigure SSinhale.with maxSpeed: 29 @14:42:43.32 SS.seek 25ml @14:43:44.20 Stroke completed -- waiting for pressure to equalize ... @14:43:48.07 Intake.select :close @14:43:48.23 SSV.dial :exhaust @14:43:49.02 Exhaust.select :open @14:43:49.13 SS.reconfigure SSexhale @14:43:49.18 SS.seek 0ml @14:43:58.49 Exhaust.select :close @14:43:58.61 SSV.dial CBV @14:43:58.64
CBV.dial SSV @14:43:59.42 Allowing 130s for 25.0ml of remaining 575.0ml @14:43:59.43 Intake.select :open @14:44:01.61 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:44:01.62 SS.reconfigure SSinhale.with maxSpeed: 29 @14:44:01.67 SS.seek 25ml @14:45:02.29 Stroke completed -- waiting for pressure to equalize ... @14:45:06.14 Intake.select :close @14:45:06.30 SSV.dial :exhaust @14:45:07.08 Exhaust.select :open @14:45:07.19 SS.reconfigure SSexhale @14:45:07.24 SS.seek 0ml @14:45:16.43 Exhaust.select :close @14:45:16.55 SSV.dial CBV @14:45:16.58
CBV.dial SSV @14:45:17.38 Allowing 130s for 25.0ml of remaining 550.0ml @14:45:17.39 Intake.select :open @14:45:19.57 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:45:19.58 SS.reconfigure SSinhale.with maxSpeed: 29 @14:45:19.63 SS.seek 25ml @14:46:20.27 Stroke completed -- waiting for pressure to equalize ... @14:46:24.75 Intake.select :close @14:46:24.91 SSV.dial :exhaust @14:46:25.71 Exhaust.select :open @14:46:25.82 SS.reconfigure SSexhale @14:46:25.87 SS.seek 0ml @14:46:35.12 Exhaust.select :close @14:46:35.24 SSV.dial CBV @14:46:35.27
CBV.dial SSV @14:46:36.08 Allowing 130s for 25.0ml of remaining 525.0ml @14:46:36.09 Intake.select :open @14:46:38.26 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:46:38.28 SS.reconfigure SSinhale.with maxSpeed: 29 @14:46:38.33 SS.seek 25ml @14:47:39.20 Stroke completed -- waiting for pressure to equalize ... @14:47:43.71 Intake.select :close @14:47:43.87 SSV.dial :exhaust @14:47:44.67 Exhaust.select :open @14:47:44.78 SS.reconfigure SSexhale @14:47:44.84 SS.seek 0ml @14:47:53.88 Exhaust.select :close @14:47:54.00 SSV.dial CBV @14:47:54.03
CBV.dial SSV @14:47:54.81 Allowing 130s for 25.0ml of remaining 500.0ml @14:47:54.82 Intake.select :open @14:47:56.99 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:47:57.01 SS.reconfigure SSinhale.with maxSpeed: 29 @14:47:57.06 SS.seek 25ml @14:48:37.40 Vacuum too high -- waiting up to 40s @14:48:38.50 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:48:38.52 SS.reconfigure SSinhale.with maxSpeed: 26 @14:48:38.57 SS.seek 25ml @14:49:01.32 Stroke completed -- waiting for pressure to equalize ... @14:49:05.82 Intake.select :close @14:49:05.98 SSV.dial :exhaust @14:49:06.76 Exhaust.select :open @14:49:06.87 SS.reconfigure SSexhale @14:49:06.92 SS.seek 0ml @14:49:15.71 Exhaust.select :close @14:49:15.83 SSV.dial CBV @14:49:15.86
CBV.dial SSV @14:49:16.66 Allowing 130s for 25.0ml of remaining 475.0ml @14:49:16.67 Intake.select :open @14:49:18.84 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:49:18.86 SS.reconfigure SSinhale.with maxSpeed: 26 @14:49:18.91 SS.seek 25ml @14:50:26.73 Stroke completed -- waiting for pressure to equalize ... @14:50:31.22 Intake.select :close @14:50:31.38 SSV.dial :exhaust @14:50:32.17 Exhaust.select :open @14:50:32.28 SS.reconfigure SSexhale @14:50:32.33 SS.seek 0ml @14:50:41.66 Exhaust.select :close @14:50:41.78 SSV.dial CBV @14:50:41.81
CBV.dial SSV @14:50:42.61 Allowing 130s for 25.0ml of remaining 450.0ml @14:50:42.62 Intake.select :open @14:50:44.79 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:50:44.81 SS.reconfigure SSinhale.with maxSpeed: 26 @14:50:44.86 SS.seek 25ml @14:51:52.46 Stroke completed -- waiting for pressure to equalize ... @14:51:56.96 Intake.select :close @14:51:57.12 SSV.dial :exhaust @14:51:57.92 Exhaust.select :open @14:51:58.03 SS.reconfigure SSexhale @14:51:58.08 SS.seek 0ml @14:52:07.25 Exhaust.select :close @14:52:07.36 SSV.dial CBV @14:52:07.39
CBV.dial SSV @14:52:08.19 Allowing 130s for 25.0ml of remaining 425.0ml @14:52:08.20 Intake.select :open @14:52:10.38 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:52:10.40 SS.reconfigure SSinhale.with maxSpeed: 26 @14:52:10.45 SS.seek 25ml @14:53:17.51 Stroke completed -- waiting for pressure to equalize ... @14:53:22.66 Intake.select :close @14:53:22.82 SSV.dial :exhaust @14:53:23.61 Exhaust.select :open @14:53:23.72 SS.reconfigure SSexhale @14:53:23.77 SS.seek 0ml @14:53:32.82 Exhaust.select :close @14:53:32.94 SSV.dial CBV @14:53:32.97
CBV.dial SSV @14:53:33.77 Allowing 130s for 25.0ml of remaining 400.0ml @14:53:33.78 Intake.select :open @14:53:35.95 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:53:35.97 SS.reconfigure SSinhale.with maxSpeed: 26 @14:53:36.02 SS.seek 25ml @14:54:43.32 Stroke completed -- waiting for pressure to equalize ... @14:54:48.45 Intake.select :close @14:54:48.61 SSV.dial :exhaust @14:54:49.41 Exhaust.select :open @14:54:49.52 SS.reconfigure SSexhale @14:54:49.57 SS.seek 0ml @14:54:58.88 Exhaust.select :close @14:54:59.00 SSV.dial CBV @14:54:59.03
CBV.dial SSV @14:54:59.83 Allowing 130s for 25.0ml of remaining 375.0ml @14:54:59.84 Intake.select :open @14:55:02.02 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:55:02.04 SS.reconfigure SSinhale.with maxSpeed: 26 @14:55:02.09 SS.seek 25ml @14:55:33.33 Vacuum too high -- waiting up to 40s @14:55:34.45 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:55:34.47 SS.reconfigure SSinhale.with maxSpeed: 23 @14:55:34.52 SS.seek 25ml @14:56:15.62 Stroke completed -- waiting for pressure to equalize ... @14:56:20.61 Intake.select :close @14:56:20.78 SSV.dial :exhaust @14:56:21.58 Exhaust.select :open @14:56:21.69 SS.reconfigure SSexhale @14:56:21.74 SS.seek 0ml @14:56:30.62 Exhaust.select :close @14:56:30.74 SSV.dial CBV @14:56:30.77
CBV.dial SSV @14:56:31.56 Allowing 130s for 25.0ml of remaining 350.0ml @14:56:31.57 Intake.select :open @14:56:33.75 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:56:33.76 SS.reconfigure SSinhale.with maxSpeed: 23 @14:56:33.82 SS.seek 25ml @14:57:50.23 Stroke completed -- waiting for pressure to equalize ... @14:57:55.37 Intake.select :close @14:57:55.53 SSV.dial :exhaust @14:57:56.31 Exhaust.select :open @14:57:56.42 SS.reconfigure SSexhale @14:57:56.47 SS.seek 0ml @14:58:05.65 Exhaust.select :close @14:58:05.77 SSV.dial CBV @14:58:05.80
CBV.dial SSV @14:58:06.61 Allowing 130s for 25.0ml of remaining 325.0ml @14:58:06.62 Intake.select :open @14:58:08.80 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:58:08.81 SS.reconfigure SSinhale.with maxSpeed: 23 @14:58:08.86 SS.seek 25ml @14:59:25.09 Stroke completed -- waiting for pressure to equalize ... @14:59:30.25 Intake.select :close @14:59:30.41 SSV.dial :exhaust @14:59:31.20 Exhaust.select :open @14:59:31.31 SS.reconfigure SSexhale @14:59:31.36 SS.seek 0ml @14:59:40.21 Exhaust.select :close @14:59:40.33 SSV.dial CBV @14:59:40.36
CBV.dial SSV @14:59:41.14 Allowing 130s for 25.0ml of remaining 300.0ml @14:59:41.15 Intake.select :open @14:59:43.32 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:59:43.34 SS.reconfigure SSinhale.with maxSpeed: 23 @14:59:43.39 SS.seek 25ml @15:00:59.84 Stroke completed -- waiting for pressure to equalize ... @15:01:05.61 Intake.select :close @15:01:05.77 SSV.dial :exhaust @15:01:06.55 Exhaust.select :open @15:01:06.66 SS.reconfigure SSexhale @15:01:07.19 SS.seek 0ml @15:01:16.07 Exhaust.select :close @15:01:16.19 SSV.dial CBV @15:01:16.22
CBV.dial SSV @15:01:17.05 Allowing 130s for 25.0ml of remaining 275.0ml @15:01:17.06 Intake.select :open @15:01:19.24 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:01:19.25 SS.reconfigure SSinhale.with maxSpeed: 23 @15:01:19.31 SS.seek 25ml @15:02:34.24 Vacuum too high -- waiting up to 40s @15:02:35.60 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:02:35.62 SS.reconfigure SSinhale.with maxSpeed: 21 @15:02:35.67 SS.seek 25ml @15:02:37.82 Stroke completed -- waiting for pressure to equalize ... @15:02:42.33 Intake.select :close @15:02:42.49 SSV.dial :exhaust @15:02:43.28 Exhaust.select :open @15:02:43.39 SS.reconfigure SSexhale @15:02:43.44 SS.seek 0ml @15:02:52.79 Exhaust.select :close @15:02:52.91 SSV.dial CBV @15:02:52.94
CBV.dial SSV @15:02:53.74 Allowing 130s for 25.0ml of remaining 250.0ml @15:02:53.75 Intake.select :open @15:02:55.92 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:02:55.94 SS.reconfigure SSinhale.with maxSpeed: 21 @15:02:55.99 SS.seek 25ml @15:04:19.35 Stroke completed -- waiting for pressure to equalize ... @15:04:25.12 Intake.select :close @15:04:25.28 SSV.dial :exhaust @15:04:26.06 Exhaust.select :open @15:04:26.17 SS.reconfigure SSexhale @15:04:26.22 SS.seek 0ml @15:04:35.38 Exhaust.select :close @15:04:35.50 SSV.dial CBV @15:04:35.53
CBV.dial SSV @15:04:36.31 Allowing 130s for 25.0ml of remaining 225.0ml @15:04:36.32 Intake.select :open @15:04:38.50 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:04:38.51 SS.reconfigure SSinhale.with maxSpeed: 21 @15:04:38.56 SS.seek 25ml @15:06:01.87 Stroke completed -- waiting for pressure to equalize ... @15:06:08.29 Intake.select :close @15:06:08.45 SSV.dial :exhaust @15:06:09.72 Exhaust.select :open @15:06:09.83 SS.reconfigure SSexhale @15:06:09.88 SS.seek 0ml @15:06:19.09 Exhaust.select :close @15:06:19.21 SSV.dial CBV @15:06:19.24
CBV.dial SSV @15:06:20.03 Allowing 130s for 25.0ml of remaining 200.0ml @15:06:20.04 Intake.select :open @15:06:22.22 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:06:22.24 SS.reconfigure SSinhale.with maxSpeed: 21 @15:06:22.29 SS.seek 25ml @15:07:45.63 Stroke completed -- waiting for pressure to equalize ... @15:07:52.02 Intake.select :close @15:07:52.18 SSV.dial :exhaust @15:07:52.96 Exhaust.select :open @15:07:53.08 SS.reconfigure SSexhale @15:07:53.13 SS.seek 0ml @15:08:02.26 Exhaust.select :close @15:08:02.38 SSV.dial CBV @15:08:02.41
CBV.dial SSV @15:08:03.22 Allowing 130s for 25.0ml of remaining 175.0ml @15:08:03.23 Intake.select :open @15:08:05.41 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:08:05.42 SS.reconfigure SSinhale.with maxSpeed: 21 @15:08:05.47 SS.seek 25ml @15:09:04.80 Vacuum too high -- waiting up to 40s @15:09:06.14 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:09:06.16 SS.reconfigure SSinhale.with maxSpeed: 19 @15:09:06.21 SS.seek 25ml @15:09:33.23 Stroke completed -- waiting for pressure to equalize ... @15:09:39.67 Intake.select :close @15:09:39.83 SSV.dial :exhaust @15:09:40.63 Exhaust.select :open @15:09:40.74 SS.reconfigure SSexhale @15:09:40.79 SS.seek 0ml @15:09:49.66 Exhaust.select :close @15:09:49.78 SSV.dial CBV @15:09:49.81
CBV.dial SSV @15:09:50.59 Allowing 130s for 25.0ml of remaining 150.0ml @15:09:50.60 Intake.select :open @15:09:52.77 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:09:52.79 SS.reconfigure SSinhale.with maxSpeed: 19 @15:09:52.84 SS.seek 25ml @15:11:25.27 Stroke completed -- waiting for pressure to equalize ... @15:11:32.16 Intake.select :close @15:11:32.32 SSV.dial :exhaust @15:11:33.11 Exhaust.select :open @15:11:33.22 SS.reconfigure SSexhale @15:11:33.27 SS.seek 0ml @15:11:42.19 Exhaust.select :close @15:11:42.30 SSV.dial CBV @15:11:42.33
CBV.dial SSV @15:11:43.14 Allowing 130s for 25.0ml of remaining 125.0ml @15:11:43.15 Intake.select :open @15:11:45.34 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:11:45.35 SS.reconfigure SSinhale.with maxSpeed: 19 @15:11:45.40 SS.seek 25ml @15:13:17.80 Stroke completed -- waiting for pressure to equalize ... @15:13:24.83 Intake.select :close @15:13:24.99 SSV.dial :exhaust @15:13:25.78 Exhaust.select :open @15:13:25.89 SS.reconfigure SSexhale @15:13:25.94 SS.seek 0ml @15:13:35.01 Exhaust.select :close @15:13:35.13 SSV.dial CBV @15:13:35.16
CBV.dial SSV @15:13:35.95 Allowing 130s for 25.0ml of remaining 100.0ml @15:13:35.96 Intake.select :open @15:13:38.13 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:13:38.15 SS.reconfigure SSinhale.with maxSpeed: 19 @15:13:38.20 SS.seek 25ml @15:14:54.92 Vacuum too high -- waiting up to 40s @15:14:56.28 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:14:56.30 SS.reconfigure SSinhale.with maxSpeed: 17 @15:14:56.35 SS.seek 25ml @15:15:13.51 Stroke completed -- waiting for pressure to equalize ... @15:15:20.55 Intake.select :close @15:15:20.71 SSV.dial :exhaust @15:15:21.50 Exhaust.select :open @15:15:21.61 SS.reconfigure SSexhale @15:15:21.67 SS.seek 0ml @15:15:30.52 Exhaust.select :close @15:15:30.64 SSV.dial CBV @15:15:30.67
CBV.dial SSV @15:15:31.45 Allowing 130s for 25.0ml of remaining 75.0ml @15:15:31.46 Intake.select :open @15:15:33.64 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:15:33.65 SS.reconfigure SSinhale.with maxSpeed: 17 @15:15:33.70 SS.seek 25ml @15:17:16.68 Stroke completed -- waiting for pressure to equalize ... @15:17:23.62 Intake.select :close @15:17:23.78 SSV.dial :exhaust @15:17:24.56 Exhaust.select :open @15:17:24.67 SS.reconfigure SSexhale @15:17:24.72 SS.seek 0ml @15:17:33.87 Exhaust.select :close @15:17:33.99 SSV.dial CBV @15:17:34.02
CBV.dial SSV @15:17:34.83 Allowing 130s for 25.0ml of remaining 50.0ml @15:17:34.84 Intake.select :open @15:17:37.01 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:17:37.03 SS.reconfigure SSinhale.with maxSpeed: 17 @15:17:37.08 SS.seek 25ml @15:19:19.65 Stroke completed -- waiting for pressure to equalize ... @15:19:27.31 Intake.select :close @15:19:27.47 SSV.dial :exhaust @15:19:28.27 Exhaust.select :open @15:19:28.38 SS.reconfigure SSexhale @15:19:28.44 SS.seek 0ml @15:19:37.38 Exhaust.select :close @15:19:37.50 SSV.dial CBV @15:19:37.53
CBV.dial SSV @15:19:38.31 Allowing 130s for 25.0ml of remaining 25.0ml @15:19:38.32 Intake.select :open @15:19:40.49 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:19:40.51 SS.reconfigure SSinhale.with maxSpeed: 17 @15:19:40.56 SS.seek 24.97ml @15:21:23.71 Stroke completed -- waiting for pressure to equalize ... @15:21:32.03 Intake.select :close @15:21:32.19 SSV.dial :exhaust @15:21:32.98 Exhaust.select :open @15:21:33.09 SS.reconfigure SSexhale @15:21:33.15 SS.seek 0ml @15:21:42.21 Exhaust.select :close @15:21:42.33 Sampled 1500.0ml @15:21:42.35 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @15:21:42.42 CSV.dial :waste @15:21:42.99 CS.seek 0ml @15:21:43.09 CSV.dial :air @15:21:43.13 SSV.dial CBV,avoiding: :exhaust @15:21:43.16
CBV.dial SSV @15:21:43.94 CS.seek 10ml @15:21:45.09
CTV.dial :air @15:21:45.86 delay 15 seconds @15:22:00.89 SSV.dial :spit,avoiding: :exhaust @15:22:02.22 CTV.dial CSV,avoiding: :intake @15:22:02.28
SS.seek 0ml @15:22:02.41 SSV.dial CBV,avoiding: :exhaust @15:22:07.36 CBV.dial between SSV and waste2 @15:22:07.78 CTV.dial CSV,avoiding: :air @15:22:07.80
CSV.dial CTV @15:22:08.27 CS.reconfigure CSpressurize @15:22:08.32 CS.seek 0ml @15:22:33.98 BubblePoint: Intake @68.6psia, Outlet @12.9psia @15:22:33.99 CBV.dial SSV @15:22:34.34 SS.reconfigure SSconfig @15:22:34.39 SS.seek 10ml @15:22:39.91 delay 15 seconds @15:22:55.06 Evacuated: Intake @34.4psia, Outlet @-0.8psia @15:22:55.08 SSV.dial :spit,avoiding: :exhaust @15:22:56.42 SS.reconfigure SSexhale @15:22:56.47 SS.seek 0ml @15:23:01.49 SS.reconfigure SSfast @15:23:01.54 SS.seek 5ml @15:23:04.22 SSV.dial CBV,avoiding: :exhaust @15:23:04.25
CBV.dial SSV @15:23:05.59 CTV.dial :air,avoiding: :intake @15:23:06.92 CSV.dial CTV @15:23:06.97
SS.seek 10ml @15:23:07.96 CSV.dial :air @15:23:08.43 CS.reconfigure CSfast @15:23:08.48 CS.seek 0ml @15:23:09.41
SSV.dial :spit,avoiding: :exhaust @15:23:10.04 CS.coast @15:23:10.75
SS.reconfigure SSexhale @15:23:10.81 SS.seek 0ml @15:23:15.73 SS.reconfigure SSfast @15:23:15.79 SS.seek 5ml @15:23:18.45 SSV.dial CBV,avoiding: :exhaust @15:23:18.48
CBV.dial SSV @15:23:20.82 SSV.dial :spit,avoiding: :exhaust @15:23:22.17 SS.reconfigure SSconfig @15:23:22.22 SS.seek 0ml @15:23:25.37 SS.coast @15:23:25.42 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @15:23:25.43 Intake.select :close @15:23:25.51 CSV.dial :waste @15:23:25.64 SSV.dial CBV,avoiding: :exhaust @15:23:25.67
CBV.dial SSV @15:23:26.32 CS.seek 0ml @15:23:26.39 CSV.dial :air @15:23:27.03
CTV.dial :air @15:23:27.04 delay 15 seconds @15:23:27.15 CS.seek 10ml @15:23:42.07
SSV.dial :spit,avoiding: :exhaust @15:23:43.41 CTV.dial CSV,avoiding: :intake @15:23:43.45
SS.reconfigure SSexhale @15:23:43.56 SS.seek 0ml @15:23:43.64 SSV.dial CBV,avoiding: :exhaust @15:23:50.86 CBV.dial between SSV and waste2 @15:23:51.29 CSV.dial between air and CTV @15:23:51.62 CS.seek 8.38ml @15:23:56.20 CTV.dial CSV,avoiding: :air @15:23:56.23
CSV.dial CTV @15:23:56.57 CS.reconfigure CSpressurize @15:23:56.62 CS.seek 0ml @15:24:18.70 CSV.dial :air @15:24:19.19 CS.reconfigure CSfast @15:24:19.24 CS.seek 10ml @15:24:42.17 CSV.dial between air and CTV @15:24:42.52 CS.seek 3.123ml @15:24:58.83 CTV.dial CSV,avoiding: :air @15:24:58.86
CSV.dial CTV @15:24:59.17 CS.reconfigure CSpressurize @15:24:59.23 CS.seek 0ml @15:25:06.95 BubblePoint: Intake @67.8psia, Outlet @12.5psia @15:25:06.97 CBV.dial SSV @15:25:07.33 SS.reconfigure SSconfig @15:25:07.38 SS.seek 10ml @15:25:12.80 delay 15 seconds @15:25:27.95 Evacuated: Intake @38.6psia, Outlet @13.7psia @15:25:27.97 SSV.dial :spit,avoiding: :exhaust @15:25:29.30 SS.reconfigure SSexhale @15:25:29.35 SS.seek 0ml @15:25:34.28 SS.reconfigure SSfast @15:25:34.33 SS.seek 5ml @15:25:37.02 SSV.dial CBV,avoiding: :exhaust @15:25:37.05
CBV.dial SSV @15:25:38.39 CTV.dial :air,avoiding: :intake @15:25:39.72 CSV.dial CTV @15:25:39.77
SS.seek 10ml @15:25:40.76 CSV.dial :air @15:25:41.23 CS.reconfigure CSfast @15:25:41.29 CS.seek 0ml @15:25:42.33
SSV.dial :spit,avoiding: :exhaust @15:25:43.22 CS.coast @15:25:43.68
SS.reconfigure SSexhale @15:25:43.73 SS.seek 0ml @15:25:48.95 SS.reconfigure SSfast @15:25:49.01 SS.seek 5ml @15:25:51.47 SSV.dial CBV,avoiding: :exhaust @15:25:51.50
CBV.dial SSV @15:25:53.84 SSV.dial :spit,avoiding: :exhaust @15:25:55.19 SS.reconfigure SSconfig @15:25:55.24 SS.seek 0ml @15:25:58.41 SS.coast @15:25:58.46 Lysing: Adding 1.5ml lysis @15:25:58.47 CBV.dial between CSV and waste1,avoiding: :DBV @15:25:59.95 CTV.dial CSV @15:26:01.28 CSV.dial CSR @15:26:02.15 Pulling 1ml of CSR.lysis @15:26:02.16 CSR.select :lysis @15:26:02.27 CS.reconfigure CSconfig @15:26:02.33 CS.seek 1ml @15:26:05.86 delay 5 seconds @15:26:10.88 CSR.select :air @15:26:11.07 CS.seek 6ml @15:26:25.02 delay 2 seconds @15:26:27.03 CSV.dial :waste1 @15:26:28.74 CS.reconfigure CSfast @15:26:28.86 CS.seek :empty @15:26:43.02 CSV.dial CSR @15:26:44.37 Pulling 1.5ml of CSR.lysis @15:26:44.38 CSR.select :lysis @15:26:44.51 CS.reconfigure CSconfig @15:26:44.56 CS.seek 1.5ml @15:26:49.42 delay 5 seconds @15:26:54.44 CSR.select :air @15:26:54.63 CS.seek 4.5ml @15:27:03.20 delay 2 seconds @15:27:05.22 CSV.dial CTV @15:27:05.70 CS.seek 1.275ml @15:27:14.90 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @15:27:14.92 CH.seek 85C,8:00 @15:29:49.46 CH.hold 5:00,85C @15:34:49.63 Collection Puck Heater at 85.1C after lysing @15:34:49.65 CS.seek 0ml @15:34:53.87 CTV.dial between spare and air,avoiding: :intake @15:34:54.94 CSV.dial :air @15:34:55.41 CS.seek 5ml @15:35:09.20 CSV.dial CTV @15:35:09.66 delay 2 seconds @15:35:11.68 CSV.dial :air @15:35:12.15 delay 2 seconds @15:35:14.18 CSV.dial CTV @15:35:14.65 delay 2 seconds @15:35:16.67 CSV.dial :air @15:35:17.15 delay 2 seconds @15:35:19.18 CS.seek 0ml @15:35:32.90 Recovering Lysate into Collection syringe @15:35:32.91 CSV.dial CBV @15:35:34.04 CBV.dial CSV @15:35:34.45 CS.seek 5ml @15:35:48.24 delay 5 seconds @15:35:53.26 CTV.dial :air @15:35:53.84 CS.seek 7ml @15:36:00.15 delay 5 seconds @15:36:05.17 CSV.dial :air2 @15:36:05.66 CS.seek 10ml @15:36:14.27 CSV.dial between air and CTV,avoiding: CBV @15:36:15.43 Lysing Complete @15:36:15.51 Storing sh1 puck in Collection Clamp to tube 1 @15:36:15.70 SC.reconfigure SCtight @15:36:15.75 SC.seek 1 @15:36:17.88 SC.reconfigure SCloose @15:36:18.11 Elbow.reconfigure TightElbow @15:36:18.16 Elbow.seek 1 @15:36:20.64 Forearm.reconfigure TightForearm @15:36:20.69 Forearm.seek 1 + 700 counts @15:36:21.75 SE.reconfigure SEpick @15:36:21.83 SE.seek beamBreak @15:36:23.79 SE.configure SEconfig @15:36:23.97 Elbow.reconfigure LimpElbow @15:36:24.02 Forearm.reconfigure LooseForearm @15:36:24.07 Forearm.seek 1 @15:36:24.40 Elbow.hold @15:36:24.47 Elbow.reconfigure TightElbow @15:36:24.58 SE.seek 11.08 Pucks below Top Plate @15:36:25.17 Forearm.force -20 @15:36:25.21 Forearm.seek 1 + 332 counts @15:36:25.54 Forearm.reconfigure TightForearm @15:36:25.59 Forearm.seek 1 + 700 counts @15:36:25.85 SE.seek 12.08 Pucks below Top Plate @15:36:26.49 Forearm.reconfigure LooseForearm @15:36:26.54 Forearm.seek :retract @15:36:27.47 Elbow.seek CC @15:36:29.08 Elbow.reconfigure LooseElbow @15:36:29.18 Forearm.seek CC @15:36:30.14 Hand.close @15:36:30.73 CC.seek :open @15:36:35.22 Forearm.seek :retract @15:36:36.22 Elbow.reconfigure TightElbow @15:36:36.27 Elbow.seek 1 @15:36:38.17 Elbow.reconfigure LimpElbow @15:36:38.22 Forearm.seek 1 @15:36:39.26 Elbow.hold @15:36:39.33 Elbow.reconfigure TightElbow @15:36:39.38 Hand.open @15:36:40.10 Forearm.force -20 @15:36:40.14 Forearm.seek 1 + 349 counts @15:36:40.46 Forearm.reconfigure TightForearm @15:36:40.51 Forearm.seek 1 + 700 counts @15:36:40.92 SE.seek :down @15:36:42.08
Forearm.reconfigure LooseForearm @15:36:42.13 Forearm.seek :retract @15:36:42.59 SE.coast @15:36:43.08
SC.coast @15:36:43.12 Tranferring lysate to PRV @15:36:43.13 CSV.dial :air @15:36:43.44 CS.seek 10ml @15:36:43.52 CSV.dial between air and CTV @15:36:43.84 AIV.dial :bypass @15:36:43.93 Beginning samplePassC @15:36:43.94 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:36:44.02 Hand.open @15:36:44.86 Elbow.seek FlushPuck::Garage @15:36:46.91 Elbow.reconfigure LooseElbow @15:36:47.03 Forearm.seek :garage @15:36:48.04 Hand.close @15:36:48.86 Forearm.seek :retract @15:36:49.90 Elbow.reconfigure TightElbow @15:36:49.95 Elbow.seek CC @15:36:52.86 Elbow.reconfigure LooseElbow @15:36:52.97 Forearm.reconfigure TightForearm @15:36:53.02 Forearm.seek CC @15:36:54.28 CC.seek :closed @15:36:58.54 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.85 @15:36:58.63 Hand.open @15:36:59.29 Forearm.reconfigure LooseForearm @15:36:59.34 Forearm.seek :retract @15:37:00.33 AWV.select :air @15:37:00.35 AIV.dial :bypass @15:37:00.36 PSV.dial PSR @15:37:01.72 Pulling 1.5ml of PSR.flush @15:37:01.73 PSR.select :flush @15:37:01.86 PS.seek 1.5ml @15:37:17.96 delay 5 seconds @15:37:22.98 PSV.dial :waste1 @15:37:23.45 PS.seek 0ml @15:37:39.48 PSV.dial PSR @15:37:39.96 PSR.select :air @15:37:40.09 PS.seek 2.5ml @15:38:06.18 PRV.dial CBV @15:38:06.21
CBV.dial PRV @15:38:07.04 PSV.dial :mixloop @15:38:08.07 CTV.dial :air,avoiding: :intake @15:38:08.08 PTV.dial :PRVmixing @15:38:08.19 PS.seek 0ml @15:38:34.39 PSV.dial :air @15:38:34.87 PS.seek 2.5ml @15:39:00.95 PSV.dial :mixloop @15:39:01.43 PS.seek 0ml @15:39:27.61 PSV.dial :air @15:39:28.09 PS.seek 2.5ml @15:39:54.26 PSV.dial :mixloop @15:39:54.73 PS.seek 0ml @15:40:20.84 Garaging FlushPuck in Collection Clamp @15:40:20.92 Elbow.reconfigure TightElbow @15:40:20.97 Elbow.seek CC @15:40:21.22 Elbow.reconfigure LooseElbow @15:40:21.33 Forearm.seek CC @15:40:22.32 Hand.close @15:40:22.97 CC.seek :open @15:40:27.85 Forearm.seek :retract @15:40:28.86 Elbow.reconfigure TightElbow @15:40:28.91 Elbow.seek :garage @15:40:32.02 Elbow.reconfigure LooseElbow @15:40:32.13 Forearm.reconfigure TightForearm @15:40:32.18 Forearm.seek :garage @15:40:33.37 Hand.open @15:40:34.04 Forearm.reconfigure LooseForearm @15:40:34.09 Forearm.seek :retract @15:40:35.16 Connecting PS to CS to transfer sample @15:40:35.17 CBV.dial AIV @15:40:35.18 AIV.dial :bypass @15:40:35.20 PSV.dial :mixloop @15:40:35.21 PTV.dial :PRVmixing @15:40:35.22 PRV.dial AIV @15:40:35.24 CTV.dial :air,avoiding: :intake @15:40:35.25 Loading lyfil puck from tube 2 into Collection Clamp @15:40:35.52 SC.reconfigure SCtight @15:40:35.57 SC.seek 2 @15:40:37.71 SC.reconfigure SCloose @15:40:37.94 Elbow.reconfigure TightElbow @15:40:37.99 Elbow.seek 2 @15:40:40.05 Forearm.reconfigure TightForearm @15:40:40.10 Forearm.seek 2 + 700 counts @15:40:41.15 SE.reconfigure SEpick @15:40:41.22 SE.seek beamBreak @15:40:43.53 SE.configure SEconfig @15:40:43.70 Elbow.seek 2 @15:40:43.92 Forearm.seek 2 + 300 counts @15:40:44.18 Forearm.reconfigure LooseForearm @15:40:44.23 Hand.close @15:40:44.87 SE.seek :down @15:40:45.10
Forearm.seek :retract @15:40:46.05 Elbow.seek CC @15:40:47.00 SE.coast @15:40:47.68
Elbow.reconfigure LooseElbow @15:40:47.79 Forearm.reconfigure TightForearm @15:40:47.84 Forearm.seek CC @15:40:49.11 CC.seek :closed @15:40:53.36 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 1.84 @15:40:53.38 Hand.open @15:40:54.05 Forearm.reconfigure LooseForearm @15:40:54.09 Forearm.seek :retract @15:40:55.06 SC.coast @15:40:55.10 CTV.dial CSV,avoiding: :intake @15:40:56.43 CSV.dial CTV @15:40:56.74 CS.seek 5ml @15:41:10.50 delay 1:20 @15:42:30.53 CS.seek 2.5ml @15:42:37.90 delay 40 seconds @15:43:17.93 CS.seek 0ml @15:43:25.34 delay 1:00 @15:44:25.36 PSV.dial :air @15:44:25.85 PS.seek 2.5ml @15:44:52.05 PSV.dial :mixloop @15:44:53.01 delay 3 seconds @15:44:56.06 PSV.dial :air @15:44:56.53 delay 2 seconds @15:44:58.55 PSV.dial :mixloop @15:44:59.03 delay 3 seconds @15:45:02.05 PSV.dial :waste1 @15:45:03.37 PS.seek :empty @15:45:29.48 CBV.dial :waste1 @15:45:29.97 delay 5 seconds @15:45:34.99 CBV.dial AIV @15:45:35.48 delay 10 seconds @15:45:45.50 CBV.dial :waste1 @15:45:45.98 delay 10 seconds @15:45:56.00 CBV.dial AIV @15:45:56.49 delay 10 seconds @15:46:06.52 CTV.dial CSV,avoiding: :intake @15:46:06.53 CBV.dial between AIV and waste1 @15:46:06.89 CSV.dial :air @15:46:07.37 CS.seek 10ml @15:46:33.87 CSV.dial CTV @15:46:34.36 CS.seek 0ml @15:47:00.80 CBV.dial :waste1 @15:47:01.13 delay 10 seconds @15:47:11.16 CTV.dial :air,avoiding: :intake @15:47:12.54 Storing lyfil puck in Collection Clamp to tube 1 @15:47:12.73 SC.reconfigure SCtight @15:47:12.78 SC.seek 1 @15:47:14.91 SC.reconfigure SCloose @15:47:15.14 Elbow.reconfigure TightElbow @15:47:15.19 Elbow.seek 1 @15:47:17.08 Forearm.reconfigure TightForearm @15:47:17.13 Forearm.seek 1 + 700 counts @15:47:18.18 SE.reconfigure SEpick @15:47:18.26 SE.seek beamBreak @15:47:20.18 SE.configure SEconfig @15:47:20.36 Elbow.reconfigure LimpElbow @15:47:20.41 Forearm.reconfigure LooseForearm @15:47:20.46 Forearm.seek 1 @15:47:20.76 Elbow.hold @15:47:20.83 Elbow.reconfigure TightElbow @15:47:20.94 SE.seek 12.08 Pucks below Top Plate @15:47:21.38 Forearm.force -20 @15:47:21.42 Forearm.seek 1 + 345 counts @15:47:21.75 Forearm.reconfigure TightForearm @15:47:21.80 Forearm.seek 1 + 700 counts @15:47:22.12 SE.seek 13.08 Pucks below Top Plate @15:47:22.77 Forearm.reconfigure LooseForearm @15:47:22.82 Forearm.seek :retract @15:47:23.75 Elbow.seek CC @15:47:25.46 Elbow.reconfigure LooseElbow @15:47:25.57 Forearm.seek CC @15:47:26.53 Hand.close @15:47:27.12 CC.seek :open @15:47:31.63 Forearm.seek :retract @15:47:32.59 Elbow.reconfigure TightElbow @15:47:32.64 Elbow.seek 1 @15:47:34.52 Elbow.reconfigure LimpElbow @15:47:34.58 Forearm.seek 1 @15:47:35.61 Elbow.hold @15:47:36.18 Elbow.reconfigure TightElbow @15:47:36.24 Hand.open @15:47:36.99 Forearm.force -20 @15:47:37.04 Forearm.seek 1 + 348 counts @15:47:37.36 Forearm.reconfigure TightForearm @15:47:37.41 Forearm.seek 1 + 700 counts @15:47:37.76 SE.seek :down @15:47:38.93
Forearm.reconfigure LooseForearm @15:47:38.98 Forearm.seek :retract @15:47:39.31 SE.coast @15:47:39.89
SC.coast @15:47:39.93 PSV.dial :air @15:47:41.00 PS.seek 0.499ml @15:47:47.00 delay 3 seconds @15:47:50.01 PSV.dial :mixloop @15:47:50.46 PRV.dial :air @15:47:50.92 delay 3 seconds @15:47:53.94 PRV.dial CBV @15:47:54.42 CBV.dial AIV @15:47:54.91 PRV.dial between CBV and air @15:47:55.23 PRV.dial :air @15:47:55.63 PS.reconfigure PSslow @15:47:55.68 PS.seek 0.599ml @15:47:58.17 PRV.dial :waste2 @15:47:58.90 PS.reconfigure PSconfig @15:47:58.95 PS.seek :empty @15:48:05.93 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:48:06.00 Hand.open @15:48:06.36 Elbow.seek FlushPuck::Garage @15:48:08.29 Elbow.reconfigure LooseElbow @15:48:08.40 Forearm.seek :garage @15:48:09.40 Hand.close @15:48:10.11 Forearm.seek :retract @15:48:11.12 Elbow.reconfigure TightElbow @15:48:11.17 Elbow.seek CC @15:48:14.00 Elbow.reconfigure LooseElbow @15:48:14.11 Forearm.reconfigure TightForearm @15:48:14.16 Forearm.seek CC @15:48:15.42 CC.seek :closed @15:48:19.69 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.83 @15:48:19.76 Hand.open @15:48:20.43 Forearm.reconfigure LooseForearm @15:48:20.48 Forearm.seek :retract @15:48:21.53 Rinsing MixLoop with GuSCN @15:48:21.54 PSV.dial :mixloop,avoiding: PBV @15:48:21.55 PTV.dial :PRVmixing,avoiding: :puck @15:48:21.56 PRV.dial :spare1 @15:48:22.64 PS.seek 1.099ml @15:48:35.15 delay 2 seconds @15:48:37.16 PRV.dial :air @15:48:38.21 PS.seek 2.5ml @15:48:53.15 PSV.dial :waste1,avoiding: PBV @15:48:54.45 PS.seek :empty @15:49:20.65 Diluting Lysate in Syringe @15:49:20.66 PSV.dial :PTV,avoiding: PBV @15:49:22.25 PTV.dial :PRV,avoiding: :puck @15:49:23.00 CBV.dial :PRV @15:49:23.01 PRV.dial :air @15:49:23.03 delay 2 seconds @15:49:25.04 PRV.dial CBV @15:49:25.51 delay 2 seconds @15:49:27.54 PS.reconfigure PSslow1 @15:49:27.59 PS.seek 0.1ml @15:49:38.90 delay 5 seconds @15:49:43.92 PRV.dial :spare1 @15:49:44.67 PS.reconfigure PSconfig @15:49:44.72 PS.seek 1ml @15:49:54.68 delay 2 seconds @15:49:56.70 PRV.dial :air @15:49:57.73 PS.seek 2.5ml @15:50:13.75 delay 2 seconds @15:50:15.77 Parking diluted lysate in Mixing Coil @15:50:15.78 PSV.dial :mixloop @15:50:16.25 PTV.dial :PRVmixing,avoiding: :puck @15:50:17.00 PRV.dial :CBV @15:50:17.55 PS.seek 1.5ml @15:50:28.50 delay 2 seconds @15:50:30.52 PSV.dial :waste1,avoiding: PBV @15:50:31.83 PS.seek :empty @15:50:47.83 Beginning samplePassF @15:50:47.91 AWV.select :air @15:50:47.93 AIV.dial :bypass @15:50:47.94 PSV.dial PSR,avoiding: PBV @15:50:48.46 Pulling 1.5ml of PSR.flush @15:50:48.47 PSR.select :flush @15:50:48.60 PS.seek 1.5ml @15:51:04.69 delay 5 seconds @15:51:09.71 PSV.dial :waste1 @15:51:10.20 PS.seek 0ml @15:51:26.14 PSV.dial PSR @15:51:26.61 PSR.select :air @15:51:26.74 PS.seek 2.5ml @15:51:52.81 PRV.dial CBV @15:51:52.84
CBV.dial PRV @15:51:52.85 PSV.dial :PTV,via: :air @15:51:54.72 CTV.dial :air,avoiding: :intake @15:51:54.73 PTV.dial :PRV,avoiding: :puck @15:51:55.50 PS.seek 0ml @15:52:21.71 PSV.dial :air @15:52:22.47 PS.seek 2.5ml @15:52:48.61 PSV.dial :PTV @15:52:49.36 PS.seek 0ml @15:53:15.59 moving diluted lysate into CBV-AIV line @15:53:15.61 PSV.dial :air @15:53:16.36 PS.seek 2.5ml @15:53:42.53 delay 2 seconds @15:53:44.54 PSV.dial :mixloop @15:53:45.01 PTV.dial :PRVmixing,avoiding: :puck @15:53:45.76 PRV.dial CBV @15:53:45.85 PS.seek 1.8ml @15:53:53.72 delay 3 seconds @15:53:56.73 PRV.dial :waste2 @15:53:57.76 PS.seek :empty @15:54:16.87 Clearing PML for modified lysate @15:54:16.88 CTV.dial :air,avoiding: :intake @15:54:16.89 PRV.dial :waste2 @15:54:16.91 PTV.dial :PRV,avoiding: :puck @15:54:17.66 delay 2 seconds @15:54:19.69 PTV.dial :PRVmixing,avoiding: :puck @15:54:20.44 delay 2 seconds @15:54:22.47 PSV.dial :PML @15:54:22.48 delay 2 seconds @15:54:24.50 PRV.dial :air @15:54:25.25 delay 2 seconds @15:54:27.34 PS.seek 1.499ml @15:54:43.34 delay 5 seconds @15:54:48.86 PS.seek 2.5ml @15:54:59.83 PSV.dial :air @15:55:00.31 PS.seek 0ml @15:55:26.38 mixing lysate with speDil @15:55:26.39 PSV.dial :PML @15:55:26.86 CBV.dial :PRV @15:55:26.87 PRV.dial :air @15:55:26.89 delay 2 seconds @15:55:28.90 PRV.dial :speDil @15:55:29.38 delay 2 seconds @15:55:31.48 PS.reconfigure PSslow1 @15:55:31.53 PS.seek 0.225ml @15:55:55.19 delay 5 seconds @15:56:00.21 PRV.dial CBV @15:56:00.98 delay 2 seconds @15:56:03.08 PS.seek 0.475ml @15:56:29.00 delay 5 seconds @15:56:34.02 PRV.dial :air @15:56:34.52 delay 2 seconds @15:56:36.56 PS.seek 0.965ml @15:57:26.56 delay 10 seconds @15:57:36.58 PRV.dial CBV @15:57:37.05 CTV.dial :air,avoiding: :intake @15:57:37.06 PSV.dial :air @15:57:37.53 PS.reconfigure PSconfig @15:57:37.58 PS.seek 2.5ml @15:57:53.88 delay 3 seconds @15:57:56.90 PSV.dial PTV @15:57:57.66 PTV.dial :PRV,avoiding: :puck @15:57:58.42 PS.seek 0ml @15:58:24.52 PSV.dial :air @15:58:25.28 PS.seek 2.5ml @15:58:51.50 delay 3 seconds @15:58:54.51 PSV.dial PTV @15:58:55.27 PTV.dial :PRV,avoiding: :puck @15:58:55.29 PS.seek 0ml @15:59:21.41 Pushing modified lysate into AIV Injection Loop @15:59:21.42 CBV.dial AIV @15:59:21.43 AIV.dial :bypass @15:59:21.44 PRV.dial CBV @15:59:21.46 PSV.dial :air @15:59:22.22 PS.seek 2.5ml @15:59:48.41 delay 3 seconds @15:59:51.44 PSV.dial :PML @15:59:51.89 PTV.dial :PRVmixing,avoiding: :puck @15:59:52.73 PS.reconfigure PSslow1 @15:59:52.78 PS.seek 1.74ml @16:01:10.38 delay 5 seconds @16:01:15.40 AIV.dial :sample @16:01:16.00 AWV.select :waste @16:01:16.17 PS.reconfigure PSslow2 @16:01:16.22 PS.seek 1.22ml @16:03:04.72 delay 15 seconds @16:03:19.74 AIV.dial :bypass @16:03:20.33 PS.reconfigure PSconfig @16:03:20.38 PS.seek 0ml @16:03:33.62 PSV.dial :air @16:03:34.09 PS.seek 2.5ml @16:04:00.34 PSV.dial :PML @16:04:00.83 PS.seek 0ml @16:04:27.03 AIV.dial :coilToMFB @16:04:27.05 AWV.select :air @16:04:27.22 Extracting Nucleic Acid from AIV @16:04:27.25
Beginning samplePassD @16:04:27.28 SPE.hold 2:46:40,55C @16:04:27.38 ASV.dial :SPEdelivery @16:04:27.45
AWV.select :air @16:04:27.46 AIV.dial :bypass @16:04:27.48 PSV.dial PSR @16:04:27.76 delay 20 seconds @16:04:28.60
Pulling 1.5ml of PSR.killMFB @16:04:28.61 PSR.select :killMFB @16:04:28.74 PS.seek 1.5ml @16:04:44.82 delay 5 seconds @16:04:47.79 primeLysateINT @16:04:47.80 AWV.select :air @16:04:47.81 AIV.dial :coilToMFB @16:04:47.83 ATV.dial AIV @16:04:49.42 AS.reconfigure ASslow1 @16:04:49.47 AS.seek 0.299ml @16:04:50.03
PSV.dial :waste1 @16:04:50.62 PS.seek 0ml @16:05:06.66 PSV.dial PSR @16:05:07.12 PSR.select :air @16:05:07.25 PS.seek 2.5ml @16:05:33.32 PRV.dial CBV @16:05:33.35
CBV.dial PRV @16:05:33.36 PSV.dial :mixloop @16:05:34.43 CTV.dial :air,avoiding: :intake @16:05:34.44 PTV.dial :PRVmixing @16:05:34.46 PS.seek 0ml @16:05:50.65 delay 10 seconds @16:06:00.56
PSV.dial PSR @16:06:00.66 ATV.dial :air2 @16:06:01.45 AS.seek 0.329ml @16:06:01.70
Pulling 1.5ml of PSR.flush @16:06:01.71 PSR.select :flush @16:06:01.84 PS.seek 1.5ml @16:06:07.75 ATV.dial :waste2 @16:06:08.29 AS.reconfigure ASnorm @16:06:08.34 AS.seek :empty @16:06:17.79
delay 5 seconds @16:06:22.82 PSV.dial :waste1 @16:06:23.30 PS.seek 0ml @16:06:25.35 Lysate primed! @16:06:25.36 AFV.select :holdingCoil @16:06:25.37 ATV.dial :air2 @16:06:25.97 AS.reconfigure ASslow1 @16:06:26.02 AS.seek 0.069ml @16:06:39.29
PSV.dial PSR @16:06:39.77 PSR.select :air @16:06:39.90 PS.seek 2.5ml @16:06:40.45 delay 3 seconds @16:06:43.46 ATV.dial :AIV @16:06:44.25 AS.seek 0.469ml @16:07:05.98 PRV.dial CBV @16:07:06.01
CBV.dial PRV @16:07:06.02 PSV.dial :mixloop @16:07:07.07 CTV.dial :air,avoiding: :intake @16:07:07.08 PTV.dial :PRVmixing @16:07:07.10 PS.seek 0ml @16:07:33.25 PSV.dial PSR @16:07:34.36 Pulling 1.5ml of PSR.flush @16:07:34.37 PSR.select :flush @16:07:34.51 PS.seek 1.5ml @16:07:50.60 delay 5 seconds @16:07:55.62 PSV.dial :waste1 @16:07:56.11 PS.seek 0ml @16:08:04.96 delay 5 seconds @16:08:10.05 Solid Phase Extraction Heater at 50.5C @16:08:10.06 ATV.dial ASV @16:08:10.45 ASV.dial :SPEdelivery @16:08:10.53 AS.seek 0.041ml @16:08:12.16
PSV.dial PSR @16:08:12.62 PSR.select :air @16:08:12.75 PS.seek 2.5ml @16:08:38.95 PRV.dial CBV @16:08:38.98
CBV.dial PRV @16:08:38.99 PSV.dial :mixloop @16:08:40.15 CTV.dial :air,avoiding: :intake @16:08:40.16 PTV.dial :PRVmixing @16:08:40.18 PS.seek 0ml @16:09:06.37 PSV.dial PSR @16:09:07.44 Pulling 1.5ml of PSR.flush @16:09:07.45 PSR.select :flush @16:09:07.58 PS.seek 1.5ml @16:09:23.60 delay 5 seconds @16:09:28.62 PSV.dial :waste1 @16:09:29.11 PS.seek 0ml @16:09:36.90 delay 10 seconds @16:09:45.12
PSV.dial PSR @16:09:45.59 PSR.select :air @16:09:45.72 PS.seek 2.5ml @16:09:46.92 rinseLysateLineINT @16:09:46.93 AFV.select :carrierSolution @16:09:47.04 delay 2 seconds @16:09:49.06 AS.reconfigure ASconfig @16:09:49.11 AS.seek 1ml @16:10:12.29 PRV.dial CBV @16:10:12.32
CBV.dial PRV @16:10:12.34 PSV.dial :mixloop @16:10:13.37 CTV.dial :air,avoiding: :intake @16:10:13.38 PTV.dial :PRVmixing @16:10:13.41 PS.reconfigure PSslow @16:10:13.46 PS.seek 1.2ml @16:10:40.35 PSV.dial :waste1 @16:10:41.67 PS.reconfigure PSconfig @16:10:41.72 PS.seek 0ml @16:10:44.49 delay 1 second @16:10:45.51 AFV.select :holdingCoil @16:10:45.63 delay 1 second @16:10:46.65 ATV.dial AIV @16:10:47.28 AIV.dial :coilToMFB @16:10:47.29 AWV.select :waste @16:10:47.40 AS.reconfigure ASslow0 @16:10:47.45 AS.seek 0ml @16:10:54.70
PSV.dial :air @16:10:55.74 PS.seek 2.5ml @16:11:21.85 PSV.dial :mixloop @16:11:22.29 PS.reconfigure PSslow @16:11:22.35 PS.seek 0ml @16:12:13.41 PSV.dial :air @16:12:13.89 PS.reconfigure PSconfig @16:12:13.95 PS.seek 2.5ml @16:12:28.31 ATV.dial :air2 @16:12:28.97 AS.reconfigure ASslow1 @16:12:29.02 AS.seek 0.05ml @16:12:39.41 ATV.dial :AIV @16:12:40.23
PSV.dial :mixloop @16:12:40.27 AS.seek 0.01ml @16:12:40.74
PS.reconfigure PSslow @16:12:40.80 PS.seek 0ml @16:12:48.65 ATV.dial :waste @16:12:50.17 AS.seek :empty @16:12:52.60 ethanol wash @16:12:52.61 ATV.dial :air2 @16:12:53.78 AS.reconfigure ASslow2 @16:12:53.83 AS.seek 0.009ml @16:13:04.26 delay 3 seconds @16:13:07.29 Pulling 0.4ml of Ethanol Wash @16:13:07.30 ATV.dial :ethanol @16:13:08.08 AS.reconfigure ASslow1 @16:13:08.13 AS.seek 0.409ml @16:13:31.86
Cleaning Collection Syringe @16:13:31.87 CSV.dial CSR @16:13:32.38 Pulling 4ml of CSR.flush @16:13:32.39 CSR.select :flush @16:13:32.52 CS.seek 4ml @16:13:43.81 delay 5 seconds @16:13:48.83 CSR.select :air @16:13:48.97 CS.seek 10ml @16:14:05.31 CSV.dial CTV @16:14:05.34
CTV.dial CSV @16:14:06.73 CBV.dial :waste1 @16:14:07.24 CS.seek 0ml @16:14:28.90 delay 3 seconds @16:14:31.92 ATV.dial :air1 @16:14:32.31 delay 3 seconds @16:14:33.70
CSV.dial CSR @16:14:34.23 Pulling 4ml of CSR.flush @16:14:34.24 CSR.select :flush @16:14:34.37 CS.seek 4ml @16:14:35.40 AS.seek 0.434ml @16:14:40.76 delay 3 seconds @16:14:43.78 ATV.dial :ethanol @16:14:44.38 AS.seek 0.419ml @16:14:45.62
delay 5 seconds @16:14:47.67 delay 3 seconds @16:14:50.64
CSR.select :air @16:14:50.70 ATV.dial ASV @16:14:50.77
CS.seek 10ml @16:14:51.96 AS.seek 0.092ml @16:15:07.03 CSV.dial CTV @16:15:07.06
CTV.dial CSV @16:15:07.56 CBV.dial :waste1 @16:15:07.58 CS.seek 0ml @16:15:34.64 Garaging FlushPuck in Collection Clamp @16:15:34.72 Elbow.reconfigure TightElbow @16:15:34.77 Elbow.seek CC @16:15:35.03 Elbow.reconfigure LooseElbow @16:15:35.14 Forearm.seek CC @16:15:36.15 Hand.close @16:15:36.77 CC.seek :open @16:15:41.17 Forearm.seek :retract @16:15:42.16 Elbow.reconfigure TightElbow @16:15:42.21 Elbow.seek :garage @16:15:45.34 Elbow.reconfigure LooseElbow @16:15:45.45 Forearm.reconfigure TightForearm @16:15:45.50 Forearm.seek :garage @16:15:46.68 Hand.open @16:15:47.34 Forearm.reconfigure LooseForearm @16:15:47.39 Forearm.seek :retract @16:15:48.40 Whole Cell Archive sampling 1500ml @16:15:48.42 Loading wcr puck from tube 2 into Collection Clamp @16:15:48.69 SC.reconfigure SCtight @16:15:48.75 SC.seek 2 @16:15:50.96 SC.reconfigure SCloose @16:15:51.20 Elbow.reconfigure TightElbow @16:15:51.25 Elbow.seek 2 @16:15:53.41 Forearm.reconfigure TightForearm @16:15:53.47 Forearm.seek 2 + 700 counts @16:15:54.58 SE.reconfigure SEpick @16:15:54.65 SE.seek beamBreak @16:15:56.99 SE.configure SEconfig @16:15:57.17 Elbow.seek 2 @16:15:57.38 Forearm.seek 2 + 300 counts @16:15:57.65 Forearm.reconfigure LooseForearm @16:15:57.70 Hand.close @16:15:58.00 delay 3 seconds @16:15:58.30 SE.seek :down @16:15:58.54 Forearm.seek :retract @16:15:59.51 Elbow.seek CC @16:16:00.39 SE.coast @16:16:01.01 ATV.dial :waste2 @16:16:01.17 Elbow.reconfigure LooseElbow @16:16:01.28 Forearm.reconfigure TightForearm @16:16:01.34 Forearm.seek CC @16:16:01.81 AS.reconfigure ASconfig @16:16:01.86 AS.seek :empty @16:16:02.77 CC.seek :closed @16:16:07.08 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 1.89 @16:16:07.10 Hand.open @16:16:07.50 ATV.dial :air2 @16:16:07.85 Forearm.reconfigure LooseForearm @16:16:07.92 Forearm.seek :retract @16:16:07.96 AS.reconfigure ASslow1 @16:16:08.07 AS.seek 1ml @16:16:09.01 SC.coast @16:16:09.05 collectSample 1500ml w/bubble point of 55psi @16:16:09.07 Priming 7ml @16:16:09.08 Intake.select :close @16:16:09.09 Exhaust.select :close @16:16:09.11 SS.hold @16:16:09.35 Calibrating pressure sensors @16:16:09.36 CTV.dial :air @16:16:10.67 SSV.dial :spit,avoiding: :exhaust @16:16:10.70 SS.reconfigure SSfast @16:16:10.75 SS.seek 5ml @16:16:13.39 SSV.dial CBV @16:16:13.42 CBV.dial SSV @16:16:14.82 delay 3.8 seconds @16:16:18.76 SSV.dial :spit @16:16:20.10 SS.seek 0ml @16:16:22.90 SSV.dial CBV @16:16:24.23 CTV.dial :intake @16:16:25.00 SSV.dial CBV @16:16:25.03 CBV.dial SSV @16:16:25.06 Allowing 40s for 7.0ml of remaining 7.0ml @16:16:25.07 Intake.select :open @16:16:27.25 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:16:27.27 SS.reconfigure SSinhale.with maxSpeed: 150 @16:16:27.32 SS.seek 7ml @16:16:31.89 Vacuum too high -- waiting up to 40s @16:16:35.60 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:16:35.62 SS.reconfigure SSinhale.with maxSpeed: 135 @16:16:35.67 SS.seek 7ml @16:16:37.99 Stroke completed -- waiting for pressure to equalize ... @16:16:44.43 Intake.select :close @16:16:44.59 SSV.dial :exhaust @16:16:45.39 Exhaust.select :open @16:16:45.50 SS.reconfigure SSexhale @16:16:45.55 SS.seek 0ml @16:16:49.25 Exhaust.select :close @16:16:49.91 Sampling 1500.0ml @16:16:49.93 Intake.select :close @16:16:49.94 Exhaust.select :close @16:16:49.95 SS.reconfigure SSconfig @16:16:50.00 SS.hold @16:16:50.23 CTV.dial :intake @16:16:50.25 SSV.dial CBV @16:16:50.28 CBV.dial SSV @16:16:51.08 Allowing 130s for 25.0ml of remaining 1500.0ml @16:16:51.09 Intake.select :open @16:16:53.28 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:16:53.30 SS.reconfigure SSinhale.with maxSpeed: 135 @16:16:53.35 SS.seek 25ml @16:16:57.05 Vacuum too high -- waiting up to 40s @16:16:58.69 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:16:58.71 SS.reconfigure SSinhale.with maxSpeed: 122 @16:16:58.76 SS.seek 25ml @16:17:01.27 Vacuum too high -- waiting up to 40s @16:17:02.91 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:17:02.94 SS.reconfigure SSinhale.with maxSpeed: 110 @16:17:02.99 SS.seek 25ml @16:17:05.80 Vacuum too high -- waiting up to 40s @16:17:07.45 Intake @33.6psia, vacuum from 20.0 to 24.0psi @16:17:07.47 SS.reconfigure SSinhale.with maxSpeed: 99 @16:17:07.52 SS.seek 25ml @16:17:10.24 Vacuum too high -- waiting up to 40s @16:17:11.87 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:17:11.89 SS.reconfigure SSinhale.with maxSpeed: 89 @16:17:11.94 SS.seek 25ml @16:17:15.08 Vacuum too high -- waiting up to 40s @16:17:16.45 Intake @33.4psia, vacuum from 20.0 to 24.0psi @16:17:16.47 SS.reconfigure SSinhale.with maxSpeed: 80 @16:17:16.52 SS.seek 25ml @16:17:19.39 Vacuum too high -- waiting up to 40s @16:17:20.76 Intake @33.5psia, vacuum from 20.0 to 24.0psi @16:17:20.78 SS.reconfigure SSinhale.with maxSpeed: 72 @16:17:20.84 SS.seek 25ml @16:17:24.78 Vacuum too high -- waiting up to 40s @16:17:25.39 Intake @33.4psia, vacuum from 20.0 to 24.0psi @16:17:25.41 SS.reconfigure SSinhale.with maxSpeed: 65 @16:17:25.46 SS.seek 25ml @16:17:29.64 Vacuum too high -- waiting up to 40s @16:17:31.03 Intake @33.6psia, vacuum from 20.0 to 24.0psi @16:17:31.05 SS.reconfigure SSinhale.with maxSpeed: 59 @16:17:31.11 SS.seek 25ml @16:17:33.38 Stroke completed -- waiting for pressure to equalize ... @16:17:36.02 Intake.select :close @16:17:36.19 SSV.dial :exhaust @16:17:36.98 Exhaust.select :open @16:17:37.10 SS.reconfigure SSexhale @16:17:37.16 SS.seek 0ml @16:17:46.09 Exhaust.select :close @16:17:46.20 SSV.dial CBV @16:17:46.23 CBV.dial SSV @16:17:47.05 Allowing 130s for 25.0ml of remaining 1475.0ml @16:17:47.06 Intake.select :open @16:17:49.25 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:17:49.27 SS.reconfigure SSinhale.with maxSpeed: 59 @16:17:49.32 SS.seek 25ml @16:17:55.54 Vacuum too high -- waiting up to 40s @16:17:56.66 Intake @33.5psia, vacuum from 20.0 to 24.0psi @16:17:56.68 SS.reconfigure SSinhale.with maxSpeed: 53 @16:17:56.73 SS.seek 25ml @16:18:08.82 Vacuum too high -- waiting up to 40s @16:18:09.95 Intake @33.3psia, vacuum from 20.0 to 24.0psi @16:18:09.97 SS.reconfigure SSinhale.with maxSpeed: 48 @16:18:10.02 SS.seek 25ml @16:18:27.07 Stroke completed -- waiting for pressure to equalize ... @16:18:29.76 Intake.select :close @16:18:29.93 SSV.dial :exhaust @16:18:30.72 Exhaust.select :open @16:18:30.83 SS.reconfigure SSexhale @16:18:30.89 SS.seek 0ml @16:18:39.61 Exhaust.select :close @16:18:39.73 SSV.dial CBV @16:18:39.78 CBV.dial SSV @16:18:40.55 Allowing 130s for 25.0ml of remaining 1450.0ml @16:18:40.56 Intake.select :open @16:18:42.75 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:18:42.77 SS.reconfigure SSinhale.with maxSpeed: 48 @16:18:42.82 SS.seek 25ml @16:19:20.13 Stroke completed -- waiting for pressure to equalize ... @16:19:23.94 Intake.select :close @16:19:24.11 SSV.dial :exhaust @16:19:24.89 Exhaust.select :open @16:19:25.01 SS.reconfigure SSexhale @16:19:25.07 SS.seek 0ml @16:19:29.64 delay 5 seconds @16:19:33.83 Exhaust.select :close @16:19:33.95 SSV.dial CBV @16:19:33.98 CBV.dial SSV @16:19:34.66 ATV.dial ASV @16:19:34.80 Allowing 130s for 25.0ml of remaining 1425.0ml @16:19:34.81 Intake.select :open @16:19:35.69 ASV.dial :SPEdelivery @16:19:35.71 AS.seek 0.02ml @16:19:37.00 Intake @33.6psia, vacuum from 20.0 to 24.0psi @16:19:37.02 SS.reconfigure SSinhale.with maxSpeed: 48 @16:19:37.08 SS.seek 25ml @16:20:14.50 Stroke completed -- waiting for pressure to equalize ... @16:20:17.75 Intake.select :close @16:20:17.92 SSV.dial :exhaust @16:20:18.70 Exhaust.select :open @16:20:18.82 SS.reconfigure SSexhale @16:20:18.88 SS.seek 0ml @16:20:27.80 Exhaust.select :close @16:20:27.92 SSV.dial CBV @16:20:27.95 CBV.dial SSV @16:20:28.76 Allowing 130s for 25.0ml of remaining 1400.0ml @16:20:28.77 Intake.select :open @16:20:30.96 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:20:30.98 SS.reconfigure SSinhale.with maxSpeed: 48 @16:20:31.03 SS.seek 25ml @16:20:59.23 Vacuum too high -- waiting up to 40s @16:21:00.36 Intake @33.2psia, vacuum from 20.0 to 24.0psi @16:21:00.38 SS.reconfigure SSinhale.with maxSpeed: 43 @16:21:00.43 SS.seek 25ml @16:21:10.33 Stroke completed -- waiting for pressure to equalize ... @16:21:12.96 Intake.select :close @16:21:13.13 SSV.dial :exhaust @16:21:13.90 Exhaust.select :open @16:21:14.01 SS.reconfigure SSexhale @16:21:14.06 SS.seek 0ml @16:21:23.24 Exhaust.select :close @16:21:23.36 SSV.dial CBV @16:21:23.41 CBV.dial SSV @16:21:24.19 Allowing 130s for 25.0ml of remaining 1375.0ml @16:21:24.20 Intake.select :open @16:21:26.39 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:21:26.41 SS.reconfigure SSinhale.with maxSpeed: 43 @16:21:26.46 SS.seek 25ml @16:22:07.33 Stroke completed -- waiting for pressure to equalize ... @16:22:10.56 Intake.select :close @16:22:10.72 SSV.dial :exhaust @16:22:11.50 Exhaust.select :open @16:22:11.61 SS.reconfigure SSexhale @16:22:11.66 SS.seek 0ml @16:22:20.63 Exhaust.select :close @16:22:20.75 SSV.dial CBV @16:22:20.78 CBV.dial SSV @16:22:22.09 Allowing 130s for 25.0ml of remaining 1350.0ml @16:22:22.10 Intake.select :open @16:22:24.28 Intake @33.6psia, vacuum from 20.0 to 24.0psi @16:22:24.31 SS.reconfigure SSinhale.with maxSpeed: 43 @16:22:24.36 SS.seek 25ml @16:22:53.09 delay 5 seconds @16:22:58.11 ATV.dial :air2 @16:22:59.10 AS.seek 0.52ml @16:23:05.61 Stroke completed -- waiting for pressure to equalize ... @16:23:08.88 Intake.select :close @16:23:09.05 SSV.dial :exhaust @16:23:09.83 Exhaust.select :open @16:23:09.94 SS.reconfigure SSexhale @16:23:10.00 SS.seek 0ml @16:23:18.76 Exhaust.select :close @16:23:18.87 SSV.dial CBV @16:23:18.90 CBV.dial SSV @16:23:19.71 Allowing 130s for 25.0ml of remaining 1325.0ml @16:23:19.73 Intake.select :open @16:23:21.96 Intake @33.6psia, vacuum from 20.0 to 24.0psi @16:23:21.98 SS.reconfigure SSinhale.with maxSpeed: 43 @16:23:22.03 SS.seek 25ml @16:24:03.58 Stroke completed -- waiting for pressure to equalize ... @16:24:06.83 Intake.select :close @16:24:06.99 SSV.dial :exhaust @16:24:07.76 Exhaust.select :open @16:24:07.87 SS.reconfigure SSexhale @16:24:07.93 SS.seek 0ml @16:24:16.89 Exhaust.select :close @16:24:17.01 SSV.dial CBV @16:24:17.04 CBV.dial SSV @16:24:17.84 Allowing 130s for 25.0ml of remaining 1300.0ml @16:24:17.85 Intake.select :open @16:24:20.04 Intake @33.6psia, vacuum from 20.0 to 24.0psi @16:24:20.06 SS.reconfigure SSinhale.with maxSpeed: 43 @16:24:20.11 SS.seek 25ml @16:24:39.95 delay 5 seconds @16:24:44.97 ATV.dial ASV @16:24:45.89 ASV.dial :wasteR @16:24:46.26 AS.seek 0.3ml @16:25:01.41 Stroke completed -- waiting for pressure to equalize ... @16:25:05.29 Intake.select :close @16:25:05.46 SSV.dial :exhaust @16:25:06.25 Exhaust.select :open @16:25:06.36 SS.reconfigure SSexhale @16:25:06.41 SS.seek 0ml @16:25:15.19 Exhaust.select :close @16:25:15.31 SSV.dial CBV @16:25:15.34 CBV.dial SSV @16:25:16.14 Allowing 130s for 25.0ml of remaining 1275.0ml @16:25:16.15 Intake.select :open @16:25:18.34 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:25:18.36 SS.reconfigure SSinhale.with maxSpeed: 43 @16:25:18.41 SS.seek 25ml @16:25:30.81 delay 20 seconds @16:25:50.84 AS.seek 0.07ml @16:25:59.99 Stroke completed -- waiting for pressure to equalize ... @16:26:03.78 Intake.select :close @16:26:03.95 SSV.dial :exhaust @16:26:04.73 Exhaust.select :open @16:26:04.84 SS.reconfigure SSexhale @16:26:04.90 SS.seek 0ml @16:26:13.62 Exhaust.select :close @16:26:13.73 SSV.dial CBV @16:26:13.76 CBV.dial SSV @16:26:14.56 Allowing 130s for 25.0ml of remaining 1250.0ml @16:26:14.58 Intake.select :open @16:26:16.81 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:26:16.83 SS.reconfigure SSinhale.with maxSpeed: 43 @16:26:16.88 SS.seek 25ml @16:26:37.36 delay 20 seconds @16:26:57.38 ATV.dial :waste2 @16:26:58.09 AS.reconfigure ASconfig @16:26:58.14 AS.seek :empty @16:26:58.49 Stroke completed -- waiting for pressure to equalize ... @16:27:02.52 Elution @16:27:02.53 ATV.dial :air1 @16:27:03.04 Intake.select :close @16:27:03.21 SSV.dial :exhaust @16:27:03.59 AS.reconfigure ASslow1 @16:27:03.64 AS.seek 0.049ml @16:27:03.98 Exhaust.select :open @16:27:04.10 SS.reconfigure SSexhale @16:27:04.15 SS.seek 0ml @16:27:13.43 Exhaust.select :close @16:27:13.55 SSV.dial CBV @16:27:13.58 CBV.dial SSV @16:27:14.01 delay 3 seconds @16:27:14.37 Allowing 130s for 25.0ml of remaining 1225.0ml @16:27:14.38 Intake.select :open @16:27:16.57 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:27:16.59 SS.reconfigure SSinhale.with maxSpeed: 43 @16:27:16.64 SS.seek 25ml @16:27:17.02 ATV.dial :ElutionWater @16:27:17.69 AS.seek 0.109ml @16:27:30.03 delay 3 seconds @16:27:33.06 ATV.dial ASV,via: :air2 @16:27:35.09 ASV.dial :SPEelution @16:27:35.66 AFV.select :carrierSolution @16:27:35.82 AS.reconfigure ASnorm @16:27:35.88 AS.seek 0.759ml @16:27:51.54 Vacuum too high -- waiting up to 40s @16:27:52.92 Intake @33.2psia, vacuum from 20.0 to 24.0psi @16:27:52.95 SS.reconfigure SSinhale.with maxSpeed: 39 @16:27:53.00 SS.seek 25ml @16:27:59.89 Stroke completed -- waiting for pressure to equalize ... @16:28:03.78 Intake.select :close @16:28:03.95 SSV.dial :exhaust @16:28:04.73 Exhaust.select :open @16:28:04.84 SS.reconfigure SSexhale @16:28:04.89 SS.seek 0ml @16:28:08.95 delay 3 seconds @16:28:11.97 AFV.select :holdingCoil @16:28:12.65 AS.reconfigure ASslow1 @16:28:12.72 AS.seek 0.474ml @16:28:13.95 Exhaust.select :close @16:28:14.06 SSV.dial CBV @16:28:14.09 CBV.dial SSV @16:28:14.90 Allowing 130s for 25.0ml of remaining 1200.0ml @16:28:14.91 Intake.select :open @16:28:17.11 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:28:17.13 SS.reconfigure SSinhale.with maxSpeed: 39 @16:28:17.18 SS.seek 25ml @16:29:02.47 Stroke completed -- waiting for pressure to equalize ... @16:29:06.38 Intake.select :close @16:29:06.55 SSV.dial :exhaust @16:29:07.33 Exhaust.select :open @16:29:07.45 SS.reconfigure SSexhale @16:29:07.52 SS.seek 0ml @16:29:10.33 delay 5 seconds @16:29:15.42 AS.reconfigure ASslow2 @16:29:15.47 AS.seek 0.439ml @16:29:16.59 Exhaust.select :close @16:29:16.70 SSV.dial CBV @16:29:16.73 CBV.dial SSV @16:29:17.54 Allowing 130s for 25.0ml of remaining 1175.0ml @16:29:17.55 Intake.select :open @16:29:19.74 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:29:19.76 SS.reconfigure SSinhale.with maxSpeed: 39 @16:29:19.81 SS.seek 25ml @16:29:51.00 delay 1:00 @16:30:05.19 Stroke completed -- waiting for pressure to equalize ... @16:30:09.81 Intake.select :close @16:30:09.98 SSV.dial :exhaust @16:30:10.77 Exhaust.select :open @16:30:10.89 SS.reconfigure SSexhale @16:30:10.94 SS.seek 0ml @16:30:19.92 Exhaust.select :close @16:30:20.04 SSV.dial CBV @16:30:20.07 CBV.dial SSV @16:30:20.86 Allowing 130s for 25.0ml of remaining 1150.0ml @16:30:20.87 Intake.select :open @16:30:23.06 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:30:23.08 SS.reconfigure SSinhale.with maxSpeed: 39 @16:30:23.13 SS.seek 25ml @16:30:51.03 pushing eluate to holding coil @16:30:51.10 AS.seek 0.279ml @16:31:08.43 Stroke completed -- waiting for pressure to equalize ... @16:31:12.96 Intake.select :close @16:31:13.13 SSV.dial :exhaust @16:31:13.93 Exhaust.select :open @16:31:14.05 SS.reconfigure SSexhale @16:31:14.11 SS.seek 0ml @16:31:23.11 Exhaust.select :close @16:31:23.23 SSV.dial CBV @16:31:23.26 CBV.dial SSV @16:31:24.04 Allowing 130s for 25.0ml of remaining 1125.0ml @16:31:24.05 Intake.select :open @16:31:26.24 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:31:26.26 SS.reconfigure SSinhale.with maxSpeed: 39 @16:31:26.86 SS.seek 25ml @16:32:12.16 Stroke completed -- waiting for pressure to equalize ... @16:32:17.34 Intake.select :close @16:32:17.50 SSV.dial :exhaust @16:32:18.28 Exhaust.select :open @16:32:18.40 SS.reconfigure SSexhale @16:32:18.46 SS.seek 0ml @16:32:27.32 Exhaust.select :close @16:32:27.44 SSV.dial CBV @16:32:27.47 CBV.dial SSV @16:32:28.28 Allowing 130s for 25.0ml of remaining 1100.0ml @16:32:28.29 Intake.select :open @16:32:30.48 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:32:30.50 SS.reconfigure SSinhale.with maxSpeed: 39 @16:32:30.55 SS.seek 25ml @16:33:06.24 Vacuum too high -- waiting up to 40s @16:33:07.60 Intake @33.6psia, vacuum from 20.0 to 24.0psi @16:33:07.62 SS.reconfigure SSinhale.with maxSpeed: 35 @16:33:07.67 SS.seek 25ml @16:33:18.92 Stroke completed -- waiting for pressure to equalize ... @16:33:22.81 Intake.select :close @16:33:22.97 SSV.dial :exhaust @16:33:23.75 Exhaust.select :open @16:33:23.87 SS.reconfigure SSexhale @16:33:23.93 SS.seek 0ml @16:33:32.38 delay 30 seconds @16:33:33.18 Exhaust.select :close @16:33:33.30 SSV.dial CBV @16:33:33.33 CBV.dial SSV @16:33:34.14 Allowing 130s for 25.0ml of remaining 1075.0ml @16:33:34.15 Intake.select :open @16:33:36.34 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:33:36.36 SS.reconfigure SSinhale.with maxSpeed: 35 @16:33:36.41 SS.seek 25ml @16:34:02.41 ATV.dial :waste,via: :air3 @16:34:04.00 AS.reconfigure ASconfig @16:34:04.05 AS.seek :empty @16:34:20.45 Parking Bleach in LysateSource line and SPEcolumn @16:34:20.46 ATV.dial :air3 @16:34:21.24 AS.reconfigure ASslow1 @16:34:21.29 AS.seek 0.029ml @16:34:26.71 Stroke completed -- waiting for pressure to equalize ... @16:34:27.63 ATV.dial :ElutionWater @16:34:28.21 AS.seek 0.059ml @16:34:31.25 Intake.select :close @16:34:31.42 SSV.dial :exhaust @16:34:32.76 Exhaust.select :open @16:34:32.88 SS.reconfigure SSexhale @16:34:32.94 SS.seek 0ml @16:34:34.53 ATV.dial :air3 @16:34:35.13 AS.seek 0.069ml @16:34:37.43 delay 2 seconds @16:34:39.45 ATV.dial :bleach @16:34:39.91 AS.seek 0.769ml @16:34:42.07 Exhaust.select :close @16:34:42.19 SSV.dial CBV @16:34:42.22 CBV.dial SSV @16:34:43.01 Allowing 130s for 25.0ml of remaining 1050.0ml @16:34:43.02 Intake.select :open @16:34:45.21 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:34:45.23 SS.reconfigure SSinhale.with maxSpeed: 35 @16:34:45.28 SS.seek 25ml @16:35:35.35 Stroke completed -- waiting for pressure to equalize ... @16:35:39.89 Intake.select :close @16:35:40.05 SSV.dial :exhaust @16:35:40.84 Exhaust.select :open @16:35:40.96 SS.reconfigure SSexhale @16:35:41.02 SS.seek 0ml @16:35:50.07 Exhaust.select :close @16:35:50.19 SSV.dial CBV @16:35:50.22 CBV.dial SSV @16:35:51.03 Allowing 130s for 25.0ml of remaining 1025.0ml @16:35:51.04 Intake.select :open @16:35:53.23 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:35:53.25 SS.reconfigure SSinhale.with maxSpeed: 35 @16:35:53.30 SS.seek 25ml @16:36:43.55 Stroke completed -- waiting for pressure to equalize ... @16:36:48.74 Intake.select :close @16:36:48.90 SSV.dial :exhaust @16:36:49.68 Exhaust.select :open @16:36:49.79 SS.reconfigure SSexhale @16:36:49.85 SS.seek 0ml @16:36:59.08 Exhaust.select :close @16:36:59.20 SSV.dial CBV @16:36:59.23 CBV.dial SSV @16:37:00.03 Allowing 130s for 25.0ml of remaining 1000.0ml @16:37:00.04 Intake.select :open @16:37:00.99 delay 3 seconds @16:37:02.23 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:37:02.25 SS.reconfigure SSinhale.with maxSpeed: 35 @16:37:02.30 SS.seek 25ml @16:37:04.01 ATV.dial :air3 @16:37:04.56 delay 3 seconds @16:37:07.65 AS.seek 0.794ml @16:37:12.94 delay 3 seconds @16:37:15.96 ATV.dial :bleach @16:37:16.56 AS.seek 0.779ml @16:37:19.89 delay 3 seconds @16:37:22.91 ATV.dial :AIV @16:37:24.17 AS.reconfigure ASconfig @16:37:24.22 AS.seek 0.015ml @16:37:52.21 Stroke completed -- waiting for pressure to equalize ... @16:37:57.47 Intake.select :close @16:37:57.63 SSV.dial :exhaust @16:37:58.43 Exhaust.select :open @16:37:58.55 SS.reconfigure SSexhale @16:37:58.61 SS.seek 0ml @16:38:07.71 Exhaust.select :close @16:38:07.83 SSV.dial CBV @16:38:07.86 CBV.dial SSV @16:38:08.44 ATV.dial :waste @16:38:08.65 Allowing 130s for 25.0ml of remaining 975.0ml @16:38:08.66 Intake.select :open @16:38:09.83 AS.seek :empty @16:38:10.85 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:38:10.87 SS.reconfigure SSinhale.with maxSpeed: 35 @16:38:10.92 SS.seek 25ml @16:38:11.06 ATV.dial :air3 @16:38:11.70 AS.reconfigure ASslow1 @16:38:11.75 AS.seek 0.009ml @16:38:14.05 delay 2 seconds @16:38:16.06 ATV.dial :bleach @16:38:16.53 AS.seek 0.579ml @16:38:54.61 Vacuum too high -- waiting up to 40s @16:38:56.24 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:38:56.27 SS.reconfigure SSinhale.with maxSpeed: 32 @16:38:56.32 SS.seek 25ml @16:39:03.61 Stroke completed -- waiting for pressure to equalize ... @16:39:08.13 Intake.select :close @16:39:08.30 SSV.dial :exhaust @16:39:09.08 Exhaust.select :open @16:39:09.20 SS.reconfigure SSexhale @16:39:09.25 SS.seek 0ml @16:39:18.07 Exhaust.select :close @16:39:18.19 SSV.dial CBV @16:39:18.22 CBV.dial SSV @16:39:19.03 Allowing 130s for 25.0ml of remaining 950.0ml @16:39:19.04 Intake.select :open @16:39:21.22 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:39:21.24 SS.reconfigure SSinhale.with maxSpeed: 32 @16:39:21.29 SS.seek 25ml @16:40:11.46 delay 3 seconds @16:40:14.48 ATV.dial :air3 @16:40:15.02 delay 3 seconds @16:40:16.30 Stroke completed -- waiting for pressure to equalize ... @16:40:18.11 AS.seek 0.604ml @16:40:21.54 Intake.select :close @16:40:21.71 SSV.dial :exhaust @16:40:22.50 Exhaust.select :open @16:40:23.02 SS.reconfigure SSexhale @16:40:23.16 SS.seek 0ml @16:40:23.41 delay 3 seconds @16:40:26.42 ATV.dial :bleach @16:40:27.03 AS.seek 0.589ml @16:40:30.35 delay 3 seconds @16:40:32.08 Exhaust.select :close @16:40:32.19 SSV.dial CBV @16:40:32.22 CBV.dial SSV @16:40:33.03 Allowing 130s for 25.0ml of remaining 925.0ml @16:40:33.04 Intake.select :open @16:40:33.36 ATV.dial :ASV @16:40:34.33 ASV.dial :wasteR @16:40:34.90 AS.seek 0ml @16:40:35.23 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:40:35.25 SS.reconfigure SSinhale.with maxSpeed: 32 @16:40:35.30 SS.seek 25ml @16:41:30.63 Stroke completed -- waiting for pressure to equalize ... @16:41:36.43 Intake.select :close @16:41:36.60 SSV.dial :exhaust @16:41:37.39 Exhaust.select :open @16:41:37.51 SS.reconfigure SSexhale @16:41:37.56 SS.seek 0ml @16:41:46.39 Exhaust.select :close @16:41:46.51 SSV.dial CBV @16:41:46.54 CBV.dial SSV @16:41:47.33 Allowing 130s for 25.0ml of remaining 900.0ml @16:41:47.34 Intake.select :open @16:41:49.52 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:41:49.54 SS.reconfigure SSinhale.with maxSpeed: 32 @16:41:49.59 SS.seek 25ml @16:42:33.66 delay 1:00 @16:42:44.97 Stroke completed -- waiting for pressure to equalize ... @16:42:51.40 Intake.select :close @16:42:51.57 SSV.dial :exhaust @16:42:52.37 Exhaust.select :open @16:42:52.48 SS.reconfigure SSexhale @16:42:52.54 SS.seek 0ml @16:43:01.55 Exhaust.select :close @16:43:01.67 SSV.dial CBV @16:43:01.70 CBV.dial SSV @16:43:02.50 Allowing 130s for 25.0ml of remaining 875.0ml @16:43:02.51 Intake.select :open @16:43:04.70 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:43:04.72 SS.reconfigure SSinhale.with maxSpeed: 32 @16:43:04.77 SS.seek 25ml @16:43:33.68 AFV.select :carrierSolution @16:43:33.79 delay 2 seconds @16:43:35.82 AS.reconfigure ASconfig @16:43:35.87 AS.seek 1ml @16:43:49.52 Vacuum too high -- waiting up to 40s @16:43:51.71 SS.reconfigure SSinhale.with maxSpeed: 29 @16:43:51.76 SS.seek 25ml @16:44:03.13 Stroke completed -- waiting for pressure to equalize ... @16:44:08.32 Intake.select :close @16:44:08.49 SSV.dial :exhaust @16:44:09.26 Exhaust.select :open @16:44:09.37 SS.reconfigure SSexhale @16:44:09.42 SS.seek 0ml @16:44:18.79 Exhaust.select :close @16:44:18.90 SSV.dial CBV @16:44:18.93 CBV.dial SSV @16:44:19.73 Allowing 130s for 25.0ml of remaining 850.0ml @16:44:19.74 Intake.select :open @16:44:21.93 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:44:21.95 SS.reconfigure SSinhale.with maxSpeed: 29 @16:44:22.00 SS.seek 25ml @16:44:33.63 delay 1 second @16:44:34.65 AFV.select :holdingCoil @16:44:34.76 delay 1 second @16:44:35.78 ATV.dial ASV @16:44:35.80 ASV.dial :wasteR @16:44:35.82 AS.reconfigure ASslow1 @16:44:35.87 AS.seek 0ml @16:45:22.03 Stroke completed -- waiting for pressure to equalize ... @16:45:27.23 Intake.select :close @16:45:27.39 SSV.dial :exhaust @16:45:28.17 Exhaust.select :open @16:45:28.28 SS.reconfigure SSexhale @16:45:28.33 SS.seek 0ml @16:45:37.13 Exhaust.select :close @16:45:37.26 SSV.dial CBV @16:45:37.29 CBV.dial SSV @16:45:38.07 Allowing 130s for 25.0ml of remaining 825.0ml @16:45:38.08 Intake.select :open @16:45:40.31 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:45:40.33 SS.reconfigure SSinhale.with maxSpeed: 29 @16:45:40.38 SS.seek 25ml @16:46:41.27 Stroke completed -- waiting for pressure to equalize ... @16:46:47.08 Intake.select :close @16:46:47.25 SSV.dial :exhaust @16:46:48.03 Exhaust.select :open @16:46:48.14 SS.reconfigure SSexhale @16:46:48.19 SS.seek 0ml @16:46:57.31 Exhaust.select :close @16:46:57.42 SSV.dial CBV @16:46:57.45 CBV.dial SSV @16:46:58.26 Allowing 130s for 25.0ml of remaining 800.0ml @16:46:58.27 Intake.select :open @16:47:00.46 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:47:00.93 SS.reconfigure SSinhale.with maxSpeed: 29 @16:47:01.07 SS.seek 25ml @16:47:57.21 delay 20 seconds @16:48:01.67 Stroke completed -- waiting for pressure to equalize ... @16:48:08.13 Intake.select :close @16:48:08.29 SSV.dial :exhaust @16:48:09.07 Exhaust.select :open @16:48:09.19 SS.reconfigure SSexhale @16:48:09.24 SS.seek 0ml @16:48:17.23 ATV.dial :air3 @16:48:18.30 Exhaust.select :close @16:48:18.42 SSV.dial CBV @16:48:18.45 CBV.dial SSV @16:48:18.65 AS.seek 0.59ml @16:48:19.25 Allowing 130s for 25.0ml of remaining 775.0ml @16:48:19.26 Intake.select :open @16:48:21.45 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:48:21.47 SS.reconfigure SSinhale.with maxSpeed: 29 @16:48:21.52 SS.seek 25ml @16:49:10.61 Vacuum too high -- waiting up to 40s @16:49:12.26 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:49:12.28 SS.reconfigure SSinhale.with maxSpeed: 26 @16:49:12.33 SS.seek 25ml @16:49:25.38 Stroke completed -- waiting for pressure to equalize ... @16:49:30.56 Intake.select :close @16:49:30.72 SSV.dial :exhaust @16:49:31.50 Exhaust.select :open @16:49:31.61 SS.reconfigure SSexhale @16:49:31.67 SS.seek 0ml @16:49:40.52 Exhaust.select :close @16:49:40.64 SSV.dial CBV @16:49:40.67 CBV.dial SSV @16:49:41.48 Allowing 130s for 25.0ml of remaining 750.0ml @16:49:41.49 Intake.select :open @16:49:43.68 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:49:43.70 SS.reconfigure SSinhale.with maxSpeed: 26 @16:49:43.75 SS.seek 25ml @16:50:17.61 delay 3 seconds @16:50:20.63 ATV.dial :ASV @16:50:21.94 ASV.dial :wasteR @16:50:22.03 AS.seek 0.09ml @16:50:51.50 Stroke completed -- waiting for pressure to equalize ... @16:50:57.19 Intake.select :close @16:50:57.36 SSV.dial :exhaust @16:50:58.14 Exhaust.select :open @16:50:58.26 SS.reconfigure SSexhale @16:50:58.32 SS.seek 0ml @16:51:07.16 Exhaust.select :close @16:51:07.28 SSV.dial CBV @16:51:07.31 CBV.dial SSV @16:51:08.12 Allowing 130s for 25.0ml of remaining 725.0ml @16:51:08.13 Intake.select :open @16:51:10.32 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:51:10.34 SS.reconfigure SSinhale.with maxSpeed: 26 @16:51:10.40 SS.seek 25ml @16:52:02.85 delay 30 seconds @16:52:18.19 Stroke completed -- waiting for pressure to equalize ... @16:52:24.64 Intake.select :close @16:52:24.81 SSV.dial :exhaust @16:52:25.59 Exhaust.select :open @16:52:25.70 SS.reconfigure SSexhale @16:52:25.76 SS.seek 0ml @16:52:32.87 ATV.dial :extSrc @16:52:33.61 delay 3 seconds @16:52:34.52 Exhaust.select :close @16:52:34.64 SSV.dial CBV @16:52:34.67 CBV.dial SSV @16:52:35.46 Allowing 130s for 25.0ml of remaining 700.0ml @16:52:35.47 Intake.select :open @16:52:36.63 ATV.dial :ElutionWater @16:52:37.74 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:52:37.77 AS.seek 0.12ml @16:52:37.80 SS.reconfigure SSinhale.with maxSpeed: 26 @16:52:37.92 SS.seek 25ml @16:52:44.19 ATV.dial :air3 @16:52:44.87 AS.seek 0.13ml @16:52:47.16 delay 2 seconds @16:52:49.17 ATV.dial :bleach @16:52:49.64 AS.seek 0.23ml @16:53:10.05 delay 3 seconds @16:53:13.07 ATV.dial :air3 @16:53:13.60 delay 3 seconds @16:53:16.69 AS.seek 0.255ml @16:53:21.99 delay 3 seconds @16:53:25.01 ATV.dial :bleach @16:53:25.61 AS.seek 0.24ml @16:53:28.93 delay 3 seconds @16:53:31.95 ATV.dial :ASV @16:53:32.91 ASV.dial :SPEdelivery @16:53:33.29 AFV.select :carrierSolution @16:53:33.40 delay 2 seconds @16:53:35.43 AS.reconfigure ASconfig @16:53:35.49 AS.seek 0.5ml @16:53:45.69 Stroke completed -- waiting for pressure to equalize ... @16:53:50.75 delay 1 second @16:53:51.77 AFV.select :holdingCoil @16:53:52.39 AS.reconfigure ASslow1 @16:53:52.53 AS.seek 0.17ml @16:53:52.77 Intake.select :close @16:53:52.93 SSV.dial :exhaust @16:53:53.73 Exhaust.select :open @16:53:53.85 SS.reconfigure SSexhale @16:53:53.91 SS.seek 0ml @16:54:03.08 Exhaust.select :close @16:54:03.20 SSV.dial CBV @16:54:03.23 CBV.dial SSV @16:54:04.03 Allowing 130s for 25.0ml of remaining 675.0ml @16:54:04.04 Intake.select :open @16:54:06.23 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:54:06.25 SS.reconfigure SSinhale.with maxSpeed: 26 @16:54:06.30 SS.seek 25ml @16:54:58.14 Vacuum too high -- waiting up to 40s @16:54:59.24 SPE.hold 2:46:40,95C @16:54:59.29 delay 3:00 @16:55:00.07 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:55:00.09 SS.reconfigure SSinhale.with maxSpeed: 23 @16:55:00.14 SS.seek 25ml @16:55:17.55 Stroke completed -- waiting for pressure to equalize ... @16:55:24.01 Intake.select :close @16:55:24.18 SSV.dial :exhaust @16:55:24.98 Exhaust.select :open @16:55:25.09 SS.reconfigure SSexhale @16:55:25.14 SS.seek 0ml @16:55:34.39 Exhaust.select :close @16:55:34.50 SSV.dial CBV @16:55:34.53 CBV.dial SSV @16:55:35.36 Allowing 130s for 25.0ml of remaining 650.0ml @16:55:35.37 Intake.select :open @16:55:37.55 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:55:37.57 SS.reconfigure SSinhale.with maxSpeed: 23 @16:55:37.62 SS.seek 25ml @16:56:53.80 Stroke completed -- waiting for pressure to equalize ... @16:57:00.25 Intake.select :close @16:57:00.42 SSV.dial :exhaust @16:57:01.23 Exhaust.select :open @16:57:01.35 SS.reconfigure SSexhale @16:57:01.40 SS.seek 0ml @16:57:10.53 Exhaust.select :close @16:57:10.65 SSV.dial CBV @16:57:11.15 CBV.dial SSV @16:57:12.06 Allowing 130s for 25.0ml of remaining 625.0ml @16:57:12.07 Intake.select :open @16:57:14.25 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:57:14.28 SS.reconfigure SSinhale.with maxSpeed: 23 @16:57:14.33 SS.seek 25ml @16:57:59.33 AFV.select :carrierSolution @16:57:59.44 delay 2 seconds @16:58:01.47 AS.reconfigure ASconfig @16:58:01.52 AS.seek 1ml @16:58:30.47 Stroke completed -- waiting for pressure to equalize ... @16:58:37.59 Intake.select :close @16:58:37.76 SSV.dial :exhaust @16:58:38.57 Exhaust.select :open @16:58:38.69 SS.reconfigure SSexhale @16:58:38.75 SS.seek 0ml @16:58:47.61 Exhaust.select :close @16:58:47.74 SSV.dial CBV @16:58:47.77 CBV.dial SSV @16:58:48.57 Allowing 130s for 25.0ml of remaining 600.0ml @16:58:48.58 Intake.select :open @16:58:49.51 delay 1 second @16:58:50.52 AFV.select :holdingCoil @16:58:50.63 delay 1 second @16:58:50.77 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:58:50.79 SS.reconfigure SSinhale.with maxSpeed: 23 @16:58:50.84 SS.seek 25ml @16:58:51.65 ASV.dial :wasteD @16:58:52.73 AS.seek 0ml @16:59:50.52 Priming Eluate @16:59:50.53 AFV.select :carrierSolution @16:59:50.64 delay 2 seconds @16:59:52.67 AS.seek 0.5ml @17:00:07.36 Stroke completed -- waiting for pressure to equalize ... @17:00:15.05 Intake.select :close @17:00:15.21 SSV.dial :exhaust @17:00:16.01 Exhaust.select :open @17:00:16.12 SS.reconfigure SSexhale @17:00:16.18 SS.seek 0ml @17:00:21.73 delay 1 second @17:00:22.74 AFV.select :holdingCoil @17:00:22.85 ATV.dial ASV @17:00:22.86 ASV.dial :SPErecovery @17:00:23.41 Swish #1 of 2 @17:00:23.49 AS.reconfigure ASslow0 @17:00:23.55 AS.seek 0.6ml @17:00:25.04 Exhaust.select :close @17:00:25.15 SSV.dial CBV @17:00:25.18 CBV.dial SSV @17:00:26.01 Allowing 130s for 25.0ml of remaining 575.0ml @17:00:26.02 Intake.select :open @17:00:28.20 Intake @34.3psia, vacuum from 20.0 to 24.0psi @17:00:28.22 SS.reconfigure SSinhale.with maxSpeed: 23 @17:00:28.27 SS.seek 25ml @17:00:34.04 AS.seek 0.5ml @17:00:44.40 Swish #2 of 2 @17:00:44.49 AS.seek 0.6ml @17:00:55.51 AS.seek 0.5ml @17:01:05.87 delay 3 seconds @17:01:08.96 AS.reconfigure ASslow1 @17:01:09.02 AS.seek 0.684ml @17:01:44.83 Stroke completed -- waiting for pressure to equalize ... @17:01:46.34 delay 3 seconds @17:01:49.37 Eluate primed @17:01:49.38 ATV.dial :waste2 @17:01:49.99 AS.reconfigure ASnorm @17:01:50.05 AS.seek 0ml @17:01:53.23 Intake.select :close @17:01:53.39 SSV.dial :exhaust @17:01:54.18 Exhaust.select :open @17:01:54.29 SS.reconfigure SSexhale @17:01:54.35 SS.seek 0ml @17:02:03.47 Exhaust.select :close @17:02:03.59 SSV.dial CBV @17:02:03.62 CBV.dial SSV @17:02:04.43 Allowing 130s for 25.0ml of remaining 550.0ml @17:02:04.44 Intake.select :open @17:02:06.63 Intake @34.3psia, vacuum from 20.0 to 24.0psi @17:02:06.65 SS.reconfigure SSinhale.with maxSpeed: 23 @17:02:06.70 SS.seek 25ml @17:02:24.86 SPE.coast @17:02:24.95 ASV.dial :SPErecovery @17:02:24.98 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0417h1500ml.pcr @17:02:24.99 Building PCR Slug from ASV with MM2 mix, PP4 primer @17:02:25.01 Priming with carrier @17:02:25.02 ATV.dial ARV @17:02:26.02 ARV.dial :carrier @17:02:26.37 AS.seek 0.5ml @17:02:51.92 AFV.select :carrierSolution @17:02:52.04 delay 2 seconds @17:02:54.07 AS.reconfigure ASconfig @17:02:54.12 AS.seek 1ml @17:03:01.42 Vacuum too high -- waiting up to 40s @17:03:03.57 Intake @34.2psia, vacuum from 20.0 to 24.0psi @17:03:03.60 SS.reconfigure SSinhale.with maxSpeed: 21 @17:03:03.65 SS.seek 25ml @17:03:23.20 delay 1 second @17:03:24.22 AFV.select :holdingCoil @17:03:24.33 delay 1 second @17:03:25.35 ATV.dial :waste @17:03:26.74 AS.reconfigure ASnorm @17:03:26.79 AS.seek :empty @17:03:27.25 Stroke completed -- waiting for pressure to equalize ... @17:03:35.55 Intake.select :close @17:03:35.72 SSV.dial :exhaust @17:03:36.53 Exhaust.select :open @17:03:36.65 SS.reconfigure SSexhale @17:03:36.71 SS.seek 0ml @17:03:46.38 Exhaust.select :close @17:03:47.16 SSV.dial CBV @17:03:47.66 CBV.dial SSV @17:03:48.72 Allowing 130s for 25.0ml of remaining 525.0ml @17:03:48.95 Intake.select :open @17:03:52.39 Intake @34.4psia, vacuum from 20.0 to 24.0psi @17:03:52.62 SS.reconfigure SSinhale.with maxSpeed: 21 @17:03:52.86 SS.seek 25ml @17:04:17.52 AFV.select :carrierSolution @17:04:17.63 delay 2 seconds @17:04:19.66 AS.reconfigure ASconfig @17:04:19.71 AS.seek 0.5ml @17:04:48.82 delay 1 second @17:04:49.84 AFV.select :holdingCoil @17:04:49.95 Building Slug @17:04:49.96 ATV.dial :ARV @17:04:51.46 delay 2 seconds @17:04:53.48 ARV.dial :air1 @17:04:53.88 delay 2 seconds @17:04:55.97 AS.reconfigure ASslow2 @17:04:56.02 AS.seek 0.506ml @17:05:02.23 delay 2 seconds @17:05:04.25 ARV.dial :MM2 @17:05:05.68 delay 2 seconds @17:05:07.72 Adding MM2 @17:05:07.85 AS.seek 0.524ml @17:05:15.84 Stroke completed -- waiting for pressure to equalize ... @17:05:24.22 Intake.select :close @17:05:24.39 SSV.dial :exhaust @17:05:25.18 Exhaust.select :open @17:05:25.29 SS.reconfigure SSexhale @17:05:25.34 SS.seek 0ml @17:05:26.24 delay 25 seconds @17:05:34.70 Exhaust.select :close @17:05:34.82 SSV.dial CBV @17:05:34.88 CBV.dial SSV @17:05:35.68 Allowing 130s for 25.0ml of remaining 500.0ml @17:05:35.69 Intake.select :open @17:05:37.87 Intake @34.4psia, vacuum from 20.0 to 24.0psi @17:05:37.89 SS.reconfigure SSinhale.with maxSpeed: 21 @17:05:37.95 SS.seek 25ml @17:05:51.25 ARV.dial :PP4 @17:05:51.65 Adding PP4 @17:05:51.67 delay 2 seconds @17:05:53.75 AS.seek 0.53ml @17:06:00.10 delay 10 seconds @17:06:10.13 ARV.dial :air3 @17:06:10.52 adding ARV air @17:06:10.54 delay 2 seconds @17:06:12.62 AS.seek 0.536ml @17:06:19.02 delay 5 seconds @17:06:24.04 ARV.dial :carrier @17:06:25.21 delay 7 seconds @17:06:32.31 AS.seek 0.594ml @17:07:00.97 Stroke completed -- waiting for pressure to equalize ... @17:07:09.93 Intake.select :close @17:07:10.09 SSV.dial :exhaust @17:07:10.89 Exhaust.select :open @17:07:11.00 SS.reconfigure SSexhale @17:07:11.07 SS.seek 0ml @17:07:20.27 Exhaust.select :close @17:07:20.39 SSV.dial CBV @17:07:20.42 CBV.dial SSV @17:07:21.21 Allowing 130s for 25.0ml of remaining 475.0ml @17:07:21.22 Intake.select :open @17:07:23.42 Intake @34.4psia, vacuum from 20.0 to 24.0psi @17:07:23.44 SS.reconfigure SSinhale.with maxSpeed: 21 @17:07:23.49 SS.seek 25ml @17:07:30.92 delay 5 seconds @17:07:35.94 ATV.dial :ASV @17:07:36.34 delay 2 seconds @17:07:38.44 AS.seek 0.6ml @17:07:44.79 delay 4 seconds @17:07:48.81 ATV.dial ARV @17:07:49.43 delay 2 seconds @17:07:51.45 ARV.dial :carrier @17:07:51.47 delay 2 seconds @17:07:53.56 AS.seek 0.65ml @17:08:20.95 Vacuum too high -- waiting up to 40s @17:08:23.11 Intake @34.1psia, vacuum from 20.0 to 24.0psi @17:08:23.13 SS.reconfigure SSinhale.with maxSpeed: 19 @17:08:23.18 SS.seek 25ml @17:08:44.07 delay 4 seconds @17:08:48.09 ATV.dial PCR @17:08:48.77 ASV.dial :waste1 @17:08:49.50 ARV.dial :air1 @17:08:49.88 Positioning Slug by 0.324ml @17:08:49.97 AS.seek 0.326ml @17:08:51.97 Stroke completed -- waiting for pressure to equalize ... @17:09:01.60 Intake.select :close @17:09:01.77 SSV.dial :exhaust @17:09:02.57 Exhaust.select :open @17:09:02.69 SS.reconfigure SSexhale @17:09:02.75 SS.seek 0ml @17:09:11.55 Exhaust.select :close @17:09:11.67 SSV.dial CBV @17:09:11.70 CBV.dial SSV @17:09:12.49 Allowing 130s for 25.0ml of remaining 450.0ml @17:09:12.50 Intake.select :open @17:09:14.69 Intake @34.5psia, vacuum from 20.0 to 24.0psi @17:09:14.71 SS.reconfigure SSinhale.with maxSpeed: 19 @17:09:14.76 SS.seek 25ml @17:10:47.20 Stroke completed -- waiting for pressure to equalize ... @17:10:56.23 Intake.select :close @17:10:56.40 SSV.dial :exhaust @17:10:57.20 Exhaust.select :open @17:10:57.33 SS.reconfigure SSexhale @17:10:57.38 SS.seek 0ml @17:11:06.72 Exhaust.select :close @17:11:06.84 SSV.dial CBV @17:11:06.87 CBV.dial SSV @17:11:07.68 Allowing 130s for 25.0ml of remaining 425.0ml @17:11:07.69 Intake.select :open @17:11:09.88 Intake @34.5psia, vacuum from 20.0 to 24.0psi @17:11:09.90 SS.reconfigure SSinhale.with maxSpeed: 19 @17:11:09.95 SS.seek 25ml @17:12:41.58 Stroke completed -- waiting for pressure to equalize ... @17:12:51.94 Intake.select :close @17:12:52.10 SSV.dial :exhaust @17:12:52.89 Exhaust.select :open @17:12:53.00 SS.reconfigure SSexhale @17:12:53.05 SS.seek 0ml @17:13:02.44 Exhaust.select :close @17:13:02.56 SSV.dial CBV @17:13:02.59 CBV.dial SSV @17:13:03.39 Allowing 130s for 25.0ml of remaining 400.0ml @17:13:03.40 Intake.select :open @17:13:05.58 Intake @34.5psia, vacuum from 20.0 to 24.0psi @17:13:06.19 SS.reconfigure SSinhale.with maxSpeed: 19 @17:13:06.24 SS.seek 25ml @17:14:10.95 Vacuum too high -- waiting up to 40s @17:14:13.37 Intake @34.4psia, vacuum from 20.0 to 24.0psi @17:14:13.40 SS.reconfigure SSinhale.with maxSpeed: 17 @17:14:13.45 SS.seek 25ml @17:14:16.06 delay 5 seconds @17:14:21.08 ATV.dial ARV @17:14:21.90 ASV.dial :PCRlock @17:14:22.26 delay 5 seconds @17:14:27.29 Running Tt1 Profile60C300 thermocycling @17:14:27.30 PCR.program Profile60C300 @17:14:27.44 Profile60C300 should complete in < 1:45:21 @17:14:27.45 PCR.run 2:16:41.25 @17:14:43.81 Stroke completed -- waiting for pressure to equalize ... @17:14:52.84 Intake.select :close @17:14:53.01 SSV.dial :exhaust @17:14:53.79 Exhaust.select :open @17:14:53.91 SS.reconfigure SSexhale @17:14:53.98 SS.seek 0ml @17:15:03.11 Exhaust.select :close @17:15:03.23 SSV.dial CBV @17:15:03.26 CBV.dial SSV @17:15:04.06 Allowing 130s for 25.0ml of remaining 375.0ml @17:15:04.07 Intake.select :open @17:15:06.26 Intake @34.4psia, vacuum from 20.0 to 24.0psi @17:15:06.28 SS.reconfigure SSinhale.with maxSpeed: 17 @17:15:06.33 SS.seek 25ml @17:16:49.08 Stroke completed -- waiting for pressure to equalize ... @17:16:58.69 Intake.select :close @17:16:58.85 SSV.dial :exhaust @17:16:59.66 Exhaust.select :open @17:16:59.78 SS.reconfigure SSexhale @17:16:59.84 SS.seek 0ml @17:17:09.02 Exhaust.select :close @17:17:09.14 SSV.dial CBV @17:17:09.17 CBV.dial SSV @17:17:09.96 Allowing 130s for 25.0ml of remaining 350.0ml @17:17:09.98 Intake.select :open @17:17:12.16 Intake @34.5psia, vacuum from 20.0 to 24.0psi @17:17:12.18 SS.reconfigure SSinhale.with maxSpeed: 17 @17:17:12.23 SS.seek 25ml @17:18:54.87 Stroke completed -- waiting for pressure to equalize ... @17:19:05.15 Intake.select :close @17:19:05.32 SSV.dial :exhaust @17:19:06.10 Exhaust.select :open @17:19:06.22 SS.reconfigure SSexhale @17:19:06.27 SS.seek 0ml @17:19:15.68 Exhaust.select :close @17:19:15.80 SSV.dial CBV @17:19:15.83 CBV.dial SSV @17:19:16.63 Allowing 130s for 25.0ml of remaining 325.0ml @17:19:16.64 Intake.select :open @17:19:18.83 Intake @34.3psia, vacuum from 20.0 to 24.0psi @17:19:18.85 SS.reconfigure SSinhale.with maxSpeed: 17 @17:19:18.90 SS.seek 25ml @17:21:01.45 Stroke completed -- waiting for pressure to equalize ... @17:21:12.60 Intake.select :close @17:21:12.77 SSV.dial :exhaust @17:21:13.56 Exhaust.select :open @17:21:13.68 SS.reconfigure SSexhale @17:21:13.74 SS.seek 0ml @17:21:23.15 Exhaust.select :close @17:21:23.26 SSV.dial CBV @17:21:23.29 CBV.dial SSV @17:21:24.09 Allowing 130s for 25.0ml of remaining 300.0ml @17:21:24.10 Intake.select :open @17:21:26.29 Intake @34.4psia, vacuum from 20.0 to 24.0psi @17:21:26.31 SS.reconfigure SSinhale.with maxSpeed: 17 @17:21:26.36 SS.seek 25ml @17:22:28.39 Vacuum too high -- waiting up to 40s @17:22:30.82 Intake @34.5psia, vacuum from 20.0 to 24.0psi @17:22:30.84 SS.reconfigure SSinhale.with maxSpeed: 15 @17:22:30.89 SS.seek 25ml @17:23:17.80 Stroke completed -- waiting for pressure to equalize ... @17:23:28.14 Intake.select :close @17:23:28.30 SSV.dial :exhaust @17:23:29.10 Exhaust.select :open @17:23:29.21 SS.reconfigure SSexhale @17:23:29.26 SS.seek 0ml @17:23:38.56 Exhaust.select :close @17:23:39.28 SSV.dial CBV @17:23:39.31 CBV.dial SSV @17:23:40.10 Allowing 130s for 25.0ml of remaining 275.0ml @17:23:40.11 Intake.select :open @17:23:42.29 Intake @34.4psia, vacuum from 20.0 to 24.0psi @17:23:42.31 SS.reconfigure SSinhale.with maxSpeed: 15 @17:23:42.37 SS.seek 25ml @17:25:38.76 Stroke completed -- waiting for pressure to equalize ... @17:25:49.69 Intake.select :close @17:25:49.85 SSV.dial :exhaust @17:25:50.63 Exhaust.select :open @17:25:50.74 SS.reconfigure SSexhale @17:25:50.80 SS.seek 0ml @17:25:59.91 Exhaust.select :close @17:26:00.03 SSV.dial CBV @17:26:00.06 CBV.dial SSV @17:26:00.87 Allowing 130s for 25.0ml of remaining 250.0ml @17:26:00.88 Intake.select :open @17:26:03.08 Intake @34.7psia, vacuum from 20.0 to 24.0psi @17:26:03.11 SS.reconfigure SSinhale.with maxSpeed: 15 @17:26:03.16 SS.seek 25ml @17:28:00.22 Stroke completed -- waiting for pressure to equalize ... @17:28:10.90 SS.reconfigure SSconfig @17:28:10.96 SS.hold @17:28:11.14 Intake.select :close @17:28:11.31 ShallowSampler::Clogged in WCR -- progress too slow after 1275.0ml @17:28:11.33 SSV.dial :exhaust @17:28:12.10 Exhaust.select :open @17:28:12.22 SS.reconfigure SSexhale @17:28:12.28 SS.seek 0ml @17:28:21.56 Exhaust.select :close @17:28:21.67 Sampled 1275.0ml @17:28:21.69 Drying puck before fixing for 4:00 @17:28:21.70 CBV.dial :waste2b @17:28:22.49 delay 1:00 @17:29:22.52 CSV.dial :air @17:29:22.96 CS.reconfigure CSfast @17:29:23.02 CS.seek 10ml @17:29:46.59 Pumping wcr puck from tube 2 up to 55psi... @17:29:46.61 CBV.dial SSV @17:29:47.95 CBV.dial between CSV and waste2b @17:29:48.94 CSV.dial between air and CTV @17:29:49.30 CS.seek 6.855ml @17:29:57.43 CTV.dial CSV,avoiding: :air @17:29:57.46 CSV.dial CTV @17:29:58.28 CS.reconfigure CSpressurize @17:29:58.34 CS.seek 0ml @17:30:15.11 CBV.dial :waste2b @17:30:15.46 CS.reconfigure CSconfig @17:30:15.51 CS.seek :empty @17:30:17.71 delay 1:00 @17:31:17.74 CTV.dial :air,avoiding: :intake @17:31:19.07 Fix #1 of 2 (with rnal) for 2:00 @17:31:19.09 CSV.dial CSR @17:31:19.61 Pulling 2ml of CSR.rnal @17:31:19.62 CSR.select :rnal @17:31:19.75 CS.seek 2ml @17:31:25.84 delay 5 seconds @17:31:30.86 CSR.select :air @17:31:30.99 CS.reconfigure CSfast @17:31:31.05 CS.seek 10ml @17:31:49.71 Pumping wcr puck from tube 2 up to 55psi... @17:31:49.73 CBV.dial SSV @17:31:51.07 CBV.dial between CSV and waste2b @17:31:52.07 CSV.dial between air and CTV @17:31:52.82 CS.seek 6.867ml @17:32:00.93 CTV.dial CSV,avoiding: :air @17:32:00.96 CSV.dial CTV @17:32:02.34 CS.reconfigure CSpressurize @17:32:02.41 CS.seek 0ml @17:32:19.64 delay 2:00 @17:34:19.67 CBV.dial :waste2b @17:34:20.03 CS.reconfigure CSconfig @17:34:20.09 CS.seek :empty @17:34:21.87 delay 1:00 @17:35:21.90 CTV.dial :air,avoiding: :intake @17:35:23.22 Fix #2 of 2 (with rnal) for 2:00 @17:35:23.24 CSV.dial CSR @17:35:23.76 Pulling 2ml of CSR.rnal @17:35:23.77 CSR.select :rnal @17:35:23.90 CS.seek 2ml @17:35:30.00 delay 5 seconds @17:35:35.02 CSR.select :air @17:35:35.15 CS.reconfigure CSfast @17:35:35.21 CS.seek 10ml @17:35:53.90 Pumping wcr puck from tube 2 up to 55psi... @17:35:53.91 CBV.dial SSV @17:35:55.26 CBV.dial between CSV and waste2b @17:35:56.26 CSV.dial between air and CTV @17:35:56.88 CS.seek 6.785ml @17:36:05.12 CTV.dial CSV,avoiding: :air @17:36:05.15 CSV.dial CTV @17:36:06.55 CS.reconfigure CSpressurize @17:36:06.61 CS.seek 0ml @17:36:22.73 delay 2:00 @17:38:22.75 CBV.dial :waste2b @17:38:23.08 CS.reconfigure CSconfig @17:38:23.13 CS.seek :empty @17:38:25.81 delay 1:00 @17:39:25.84 CTV.dial :air,avoiding: :intake @17:39:27.21 Storing wcr puck in Collection Clamp to tube 1 @17:39:27.41 SC.reconfigure SCtight @17:39:27.46 SC.seek 1 @17:39:29.63 SC.reconfigure SCloose @17:39:30.44 Elbow.reconfigure TightElbow @17:39:30.50 Elbow.seek 1 @17:39:32.40 Forearm.reconfigure TightForearm @17:39:32.46 Forearm.seek 1 + 700 counts @17:39:33.51 SE.reconfigure SEpick @17:39:33.58 SE.seek beamBreak @17:39:35.34 SE.configure SEconfig @17:39:35.53 Elbow.reconfigure LimpElbow @17:39:35.58 Forearm.reconfigure LooseForearm @17:39:35.64 Forearm.seek 1 @17:39:35.98 Elbow.hold @17:39:36.06 Elbow.reconfigure TightElbow @17:39:36.17 SE.seek 13.08 Pucks below Top Plate @17:39:36.68 Forearm.force -20 @17:39:36.74 Forearm.seek 1 + 320 counts @17:39:37.06 Forearm.reconfigure TightForearm @17:39:37.12 Forearm.seek 1 + 700 counts @17:39:37.47 SE.seek 14.08 Pucks below Top Plate @17:39:38.14 Forearm.reconfigure LooseForearm @17:39:38.19 Forearm.seek :retract @17:39:39.11 Elbow.seek CC @17:39:40.83 Elbow.reconfigure LooseElbow @17:39:40.95 Forearm.seek CC @17:39:41.96 Hand.close @17:39:42.65 CC.seek :open @17:39:47.18 Forearm.seek :retract @17:39:48.15 Elbow.reconfigure TightElbow @17:39:48.20 Elbow.seek 1 @17:39:50.13 Elbow.reconfigure LimpElbow @17:39:50.19 Forearm.seek 1 @17:39:51.23 Elbow.hold @17:39:51.31 Elbow.reconfigure TightElbow @17:39:51.36 Hand.open @17:39:52.15 Forearm.force -20 @17:39:52.19 Forearm.seek 1 + 347 counts @17:39:52.51 Forearm.reconfigure TightForearm @17:39:52.57 Forearm.seek 1 + 700 counts @17:39:52.98 SE.seek :down @17:39:54.13 Forearm.reconfigure LooseForearm @17:39:54.19 Forearm.seek :retract @17:39:54.47 SE.coast @17:39:55.11 SC.coast @17:39:55.73 Starting finalPurge @17:39:55.74 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:39:55.83 Hand.open @17:39:56.29 Elbow.seek FlushPuck::Garage @17:39:58.25 Elbow.reconfigure LooseElbow @17:39:58.36 Forearm.seek :garage @17:39:59.38 Hand.close @17:40:00.15 Forearm.seek :retract @17:40:01.17 Elbow.reconfigure TightElbow @17:40:01.22 Elbow.seek CC @17:40:04.16 Elbow.reconfigure LooseElbow @17:40:04.27 Forearm.reconfigure TightForearm @17:40:04.32 Forearm.seek CC @17:40:05.58 CC.seek :closed @17:40:09.83 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.85 @17:40:09.92 Hand.open @17:40:10.59 Forearm.reconfigure LooseForearm @17:40:10.64 Forearm.seek :retract @17:40:11.61 Flushing the Collection Valve::Manifold @17:40:11.75 CSV.dial CSR @17:40:12.27 Pulling 5ml of CSR.flush CSR.select :flush @17:40:12.40 CS.seek 5ml @17:40:26.13 delay 5 seconds @17:40:31.15 CSR.select :air @17:40:31.34 CS.reconfigure CSfast @17:40:31.39 CS.seek 7.5ml @17:40:38.10 CSV.dial :waste @17:40:39.39 CS.reconfigure CSconfig @17:40:39.44 CS.seek 0ml @17:40:59.78 Flushing lower manifold's waste1 port @17:40:59.92 CSV.dial :flush @17:41:00.39 CS.reconfigure CSfast @17:41:00.45 CS.seek 5ml @17:41:12.72 delay 5 seconds @17:41:17.74 CSV.dial :air @17:41:18.27 CS.seek 7ml @17:41:23.75 CSV.dial CTV @17:41:23.78
CTV.dial CSV @17:41:25.21 CBV.dial :waste1 @17:41:26.00 CS.seek 0ml @17:41:42.57 Flushing lower manifold's waste2b port @17:41:42.71 CSV.dial :flush @17:41:43.47 CS.seek 5ml @17:41:55.69 delay 5 seconds @17:42:00.71 CSV.dial :air @17:42:01.87 CS.seek 7.001ml @17:42:07.35 CSV.dial CTV @17:42:07.38
CTV.dial CSV @17:42:07.85 CBV.dial :waste2b @17:42:08.64 CS.seek 0ml @17:42:25.13 Flushing upper manifold @17:42:25.27 CSV.dial :flush @17:42:26.03 CS.seek 5ml @17:42:38.25 delay 5 seconds @17:42:43.27 CSV.dial :air @17:42:43.82 CS.seek 7ml @17:42:49.35 CSV.dial CBV @17:42:49.38
CBV.dial CSV @17:42:50.61 CTV.dial :air @17:42:51.92 CS.seek 0ml @17:43:08.47 delay 7 seconds @17:43:15.50 CS.seek 3ml @17:43:23.13 CSV.dial :waste @17:43:23.59 CS.seek 0ml @17:43:31.48 CSV.dial :flush @17:43:31.94 CS.seek 3ml @17:43:39.85 delay 5 seconds @17:43:44.87 CSV.dial :air @17:43:45.43 CS.seek 5ml @17:43:50.94 CSV.dial :air @17:43:50.96 CS.seek 0ml @17:44:02.94 delay 7 seconds @17:44:09.97 CS.seek 3ml @17:44:17.81 CSV.dial :waste @17:44:18.58 CS.seek 0ml @17:44:26.38 Disinfecting external Intake with mfbkill @17:44:26.52 CSV.dial CSR @17:44:27.90 Pulling 8ml of CSR.mfbkill @17:44:27.91 CSR.select :mfbkill @17:44:28.03 CS.seek 8ml @17:44:46.94 delay 5 seconds @17:44:51.96 CSR.select :air @17:44:52.09 CS.seek 10ml @17:44:57.67 CSV.dial CBV @17:44:57.70
CBV.dial CSV @17:44:58.74 CTV.dial :intake @17:44:59.49 Intake.select :open @17:44:59.61 CS.seek 2ml @17:45:18.90 Intake.select :close @17:45:19.02 CTV.dial :air @17:45:19.79 CS.reconfigure CSconfig @17:45:19.84 CS.seek 0ml @17:45:25.91 Flushing lower manifold's waste1 port @17:45:25.92 CSV.dial CSR @17:45:27.01 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @17:45:27.14 CS.seek 4ml @17:45:38.32 delay 5 seconds @17:45:43.33 CSR.select :air @17:45:43.46 CS.reconfigure CSfast @17:45:43.51 CS.seek 10ml @17:45:57.94 CSV.dial CTV @17:45:57.97
CTV.dial CSV @17:45:59.43 CBV.dial :waste1 @17:45:59.92 CS.seek 0ml @17:46:22.89 Flushing lower manifold's waste2b port @17:46:22.90 CSV.dial CSR @17:46:23.44 Pulling 4ml of CSR.mfbkill @17:46:23.45 CSR.select :mfbkill @17:46:23.58 CS.reconfigure CSconfig @17:46:23.63 CS.seek 4ml @17:46:34.91 delay 5 seconds @17:46:39.93 CSR.select :air @17:46:40.06 CS.reconfigure CSfast @17:46:40.11 CS.seek 10ml @17:46:54.32 CSV.dial CTV @17:46:54.35
CTV.dial CSV @17:46:54.84 CBV.dial :waste2b @17:46:55.64 CS.seek 0ml @17:47:18.70 Killing upper manifold @17:47:18.71 CSV.dial CSR @17:47:19.25 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @17:47:19.38 CS.reconfigure CSconfig @17:47:19.42 CS.seek 4ml @17:47:30.57 delay 5 seconds @17:47:35.59 CSR.select :air @17:47:35.72 CS.reconfigure CSfast @17:47:35.78 CS.seek 10ml @17:47:50.07 CSV.dial CBV @17:47:50.10
CBV.dial CSV @17:47:51.18 CTV.dial :air @17:47:52.52 CS.seek 0ml @17:48:15.54 delay 7 seconds @17:48:22.57 CS.seek 3ml @17:48:30.20 CSV.dial :waste @17:48:30.66 CS.seek 0ml @17:48:38.26 Flushing upper manifold @17:48:38.40 CSV.dial :flush @17:48:38.88 CS.seek 5ml @17:48:50.85 delay 5 seconds @17:48:55.86 CSV.dial :air @17:48:56.40 CS.seek 7ml @17:49:01.95 CSV.dial CBV @17:49:01.98
CBV.dial CSV @17:49:03.04 CTV.dial :air @17:49:03.06 CS.seek 0ml @17:49:19.48 delay 7 seconds @17:49:26.50 CS.seek 3ml @17:49:34.12 CSV.dial :waste @17:49:34.60 CS.seek 0ml @17:49:42.36 CSV.dial :flush @17:49:42.83 CS.seek 3ml @17:49:50.60 delay 5 seconds @17:49:55.61 CSV.dial :air @17:49:56.14 CS.seek 5ml @17:50:01.59 CSV.dial :air @17:50:01.62 CS.seek 0ml @17:50:13.67 delay 7 seconds @17:50:20.69 CS.seek 3ml @17:50:28.34 CSV.dial :waste @17:50:29.67 CS.seek 0ml @17:50:37.26 Flushing lower manifold's waste1 port @17:50:37.40 CSV.dial :flush @17:50:37.88 CS.seek 5ml @17:50:50.14 delay 5 seconds @17:50:55.17 CSV.dial :air @17:50:55.70 CS.seek 7ml @17:51:01.26 CSV.dial CTV @17:51:01.29
CTV.dial CSV @17:51:02.71 CBV.dial :waste1 @17:51:03.21 CS.seek 0ml @17:51:19.95 Flushing lower manifold's waste2b port @17:51:20.10 CSV.dial :flush @17:51:20.87 CS.seek 5ml @17:51:32.89 delay 5 seconds @17:51:37.91 CSV.dial :air @17:51:38.61 CS.seek 7ml @17:51:44.07 CSV.dial CTV @17:51:44.10
CTV.dial CSV @17:51:44.57 CBV.dial :waste2b @17:51:45.37 CS.seek 0ml @17:52:01.73 Disinfecting external Intake with kill @17:52:01.87 CSV.dial CSR @17:52:02.39 Pulling 8ml of CSR.kill @17:52:02.40 CSR.select :kill @17:52:02.52 CS.seek 8ml @17:52:21.14 delay 5 seconds @17:52:26.16 CSR.select :air @17:52:26.30 CS.seek 10ml @17:52:31.90 CSV.dial CBV @17:52:31.93
CBV.dial CSV @17:52:33.03 CTV.dial :intake @17:52:33.79 Intake.select :open @17:52:33.91 CS.seek 2ml @17:52:53.03 Intake.select :close @17:52:53.15 CTV.dial :air @17:52:53.90 CS.reconfigure CSconfig @17:52:53.95 CS.seek 0ml @17:53:00.00 CSV.dial CSR @17:53:01.09 Pulling 5ml of CSR.flush @17:53:01.10 CSR.select :flush @17:53:01.23 CS.seek 5ml @17:53:15.08 delay 5 seconds @17:53:20.10 CSR.select :air @17:53:20.24 CS.seek 10ml @17:53:34.08 CSV.dial CBV @17:53:35.12 CS.seek 0ml @17:54:01.58 Garaging FlushPuck in Collection Clamp @17:54:01.66 Elbow.reconfigure TightElbow @17:54:01.71 Elbow.seek CC @17:54:01.96 Elbow.reconfigure LooseElbow @17:54:02.07 Forearm.seek CC @17:54:03.06 Hand.close @17:54:03.66 CC.seek :open @17:54:08.11 Forearm.seek :retract @17:54:09.70 Elbow.reconfigure TightElbow @17:54:09.75 Elbow.seek :garage @17:54:12.90 Elbow.reconfigure LooseElbow @17:54:13.02 Forearm.reconfigure TightForearm @17:54:13.07 Forearm.seek :garage @17:54:14.21 Hand.open @17:54:14.85 Forearm.reconfigure LooseForearm @17:54:14.90 Forearm.seek :retract @17:54:15.94 Starting initialPurge @17:54:15.95 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:54:16.03 Hand.open @17:54:16.35 Elbow.reconfigure TightElbow @17:54:16.40 Elbow.seek FlushPuck::Garage @17:54:16.60 Elbow.reconfigure LooseElbow @17:54:16.74 Forearm.seek :garage @17:54:17.75 Hand.close @17:54:18.55 Forearm.seek :retract @17:54:19.55 Elbow.reconfigure TightElbow @17:54:19.60 Elbow.seek CC @17:54:22.47 Elbow.reconfigure LooseElbow @17:54:22.58 Forearm.reconfigure TightForearm @17:54:22.63 Forearm.seek CC @17:54:23.95 CC.seek :closed @17:54:28.23 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 1.86 @17:54:28.31 Hand.open @17:54:28.99 Forearm.reconfigure LooseForearm @17:54:29.03 Forearm.seek :retract @17:54:30.02 Flushing the Collection Valve::Manifold @17:54:30.16 CSV.dial CSR @17:54:31.37 Pulling 5ml of CSR.flush @17:54:31.38 CSR.select :flush @17:54:31.51 CS.seek 5ml @17:54:45.36 delay 5 seconds @17:54:50.37 CSR.select :air @17:54:50.54 CS.reconfigure CSfast @17:54:50.59 CS.seek 7.5ml @17:54:57.22 CSV.dial :waste @17:54:58.51 CS.reconfigure CSconfig @17:54:58.56 CS.seek 0ml @17:55:18.76 Purging external Intake @17:55:18.90 CSV.dial :flush @17:55:19.36 CS.reconfigure CSfast @17:55:19.41 CS.seek 10ml @17:55:42.36 delay 5 seconds @17:55:47.38 CSV.dial CBV @17:55:47.41
CBV.dial CSV @17:55:48.19 CTV.dial :intake @17:55:48.95 Intake.select :open @17:55:49.07 CS.seek 0ml @17:56:12.91 Intake.select :close @17:56:13.03 CTV.dial :air @17:56:13.78 Flushing upper manifold @17:56:13.92 CSV.dial :flush @17:56:14.68 CS.seek 5ml @17:56:26.67 delay 5 seconds @17:56:31.68 CSV.dial :air @17:56:32.35 CS.seek 7ml @17:56:37.86 CSV.dial CBV @17:56:37.88
CBV.dial CSV @17:56:38.92 CTV.dial :air @17:56:38.94 CS.seek 0ml @17:56:55.30 delay 7 seconds @17:57:02.33 CS.seek 3ml @17:57:10.08 CSV.dial :waste @17:57:10.56 CS.seek 0ml @17:57:18.33 CSV.dial :flush @17:57:18.81 CS.seek 3ml @17:57:26.55 delay 5 seconds @17:57:31.57 CSV.dial :air @17:57:32.16 CS.seek 5ml @17:57:37.58 CSV.dial :air @17:57:37.60 CS.seek 0ml @17:57:49.64 delay 7 seconds @17:57:56.67 CS.seek 3ml @17:58:04.39 CSV.dial :waste @17:58:05.14 CS.seek 0ml @17:58:12.77 Garaging FlushPuck in Collection Clamp @17:58:12.85 Elbow.reconfigure TightElbow @17:58:12.90 Elbow.seek CC @17:58:13.14 Elbow.reconfigure LooseElbow @17:58:13.25 Forearm.seek CC @17:58:14.21 Hand.close @17:58:14.79 CC.seek :open @17:58:19.16 Forearm.seek :retract @17:58:20.15 Elbow.reconfigure TightElbow @17:58:20.20 Elbow.seek :garage @17:58:23.26 Elbow.reconfigure LooseElbow @17:58:23.37 Forearm.reconfigure TightForearm @17:58:23.42 Forearm.seek :garage @17:58:24.58 Hand.open @17:58:25.23 Forearm.reconfigure LooseForearm @17:58:25.28 Forearm.seek :retract @17:58:26.37 Completed [phasewithdil] begun 14:00:26 @17:58:26.39 Preparing to sleep until 14PM @17:58:26.44 deferred until MFBPCR finish @17:58:26.46
Waiting for MFBPCR til 14PM @17:58:26.49 Continuing at 14:00CEST05-Oct-18 @17:58:26.55 GoodNews.email "Then, in tube 2: phasewithdil\n/home/esp/esp2/mission/18tundeployv2dil.rb:118\n{1 => 15, 2 => 7}\nCan@17:58:26, 15.3C, 67% humidity, 14.4psia, 13.800V, 0.941A, 0.935A avg, 12.99W",Subject: "Waiting until 14:00CEST05-Oct-18" @18:36:47.14 PCR.off @18:36:48.70 PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0417h1500ml.pcr @18:36:48.77 Cleaning PCR @18:36:48.78 AFV.select :carrierSolution @18:36:48.89 delay 2 seconds @18:36:50.92 AS.reconfigure ASconfig @18:36:50.97 AS.seek 0.5ml @18:37:01.28 delay 1 second @18:37:02.30 AFV.select :holdingCoil @18:37:02.41 ASV.dial :waste1 @18:37:02.78 ATV.dial :air3 @18:37:03.99 AS.reconfigure ASslow1 @18:37:04.04 AS.seek 0.51ml @18:37:06.37 delay 2 seconds @18:37:08.39 ATV.dial :bleach @18:37:09.57 AS.seek 0.81ml @18:38:10.17 delay 3 seconds @18:38:13.19 ATV.dial :air3 @18:38:13.72 delay 3 seconds @18:38:16.81 AS.seek 0.835ml @18:38:22.09 delay 3 seconds @18:38:25.11 ATV.dial :bleach @18:38:25.71 AS.seek 0.82ml @18:38:29.01 delay 3 seconds @18:38:32.03 ARV.dial :waste @18:38:32.62 delay 3 seconds @18:38:35.64 ATV.dial :ARV @18:38:36.58 AS.reconfigure ASconfig @18:38:36.63 AS.seek 0.47ml @18:38:57.04 AFV.select :carrierSolution @18:38:57.16 delay 2 seconds @18:38:59.19 AS.seek 1ml @18:39:29.94 delay 1 second @18:39:30.96 AFV.select :holdingCoil @18:39:31.07 delay 1 second @18:39:32.10 AS.reconfigure ASfast @18:39:32.15 AS.seek 0ml @18:39:59.46 AFV.select :carrierSolution @18:39:59.58 delay 2 seconds @18:40:01.61 AS.reconfigure ASconfig @18:40:01.66 AS.seek 1ml @18:40:59.44 delay 1 second @18:41:00.45 AFV.select :holdingCoil @18:41:00.56 delay 1 second @18:41:01.59 AS.reconfigure ASfast @18:41:01.64 AS.seek 0ml @18:41:28.97 AFV.select :carrierSolution @18:41:29.09 delay 2 seconds @18:41:31.12 AS.reconfigure ASconfig @18:41:31.17 AS.seek 1ml @18:42:28.92 delay 1 second @18:42:29.93 AFV.select :holdingCoil @18:42:30.04 delay 1 second @18:42:31.07 AS.reconfigure ASfast @18:42:31.12 AS.seek 0ml @18:42:58.44 ARV.dial between carrier and air1 @18:42:58.92 ATV.dial :air3 @18:43:00.12 AS.reconfigure ASslow1 @18:43:00.17 AS.seek 0.01ml @18:43:02.48 delay 2 seconds @18:43:04.49 ATV.dial :bleach @18:43:05.09 AS.seek 0.81ml @18:45:46.24 delay 3 seconds @18:45:49.26 ATV.dial :air3 @18:45:49.80 delay 3 seconds @18:45:52.89 AS.seek 0.835ml @18:45:58.19 delay 3 seconds @18:46:01.21 ATV.dial :bleach @18:46:01.81 AS.seek 0.82ml @18:46:05.13 delay 3 seconds @18:46:08.15 ATV.dial PCR @18:46:08.55 AS.reconfigure ASconfig @18:46:08.60 AS.seek :empty @18:46:56.05 PCR.program :bleachProfile @18:46:56.11 PCR program should complete in < 2:45 @18:46:56.12 PCR.run 10:00 @18:50:04.01 PCR.off @18:50:04.03 AFV.select :carrierSolution @18:50:04.15 delay 2 seconds @18:50:06.18 AS.seek 1ml @18:51:04.06 delay 1 second @18:51:05.07 AFV.select :holdingCoil @18:51:05.18 delay 1 second @18:51:06.21 AS.reconfigure ASfast @18:51:06.26 AS.seek 0ml @18:51:33.61 delay 3 seconds @18:51:36.63 AFV.select :carrierSolution @18:51:36.74 delay 2 seconds @18:51:38.77 AS.reconfigure ASconfig @18:51:38.83 AS.seek 1ml @18:52:36.63 delay 1 second @18:52:37.64 AFV.select :holdingCoil @18:52:37.75 delay 1 second @18:52:38.78 AS.reconfigure ASfast @18:52:38.83 AS.seek 0ml @18:53:06.18 delay 3 seconds @18:53:09.20 AFV.select :carrierSolution @18:53:09.31 delay 2 seconds @18:53:11.34 AS.reconfigure ASconfig @18:53:11.40 AS.seek 1ml @18:54:09.18 delay 1 second @18:54:10.19 AFV.select :holdingCoil @18:54:10.30 delay 1 second @18:54:11.33 AS.reconfigure ASfast @18:54:11.38 AS.seek 0ml @18:54:38.82 delay 3 seconds @18:54:41.84 AFV.select :carrierSolution @18:54:41.95 delay 2 seconds @18:54:44.47 AS.reconfigure ASconfig @18:54:44.60 AS.seek 1ml @18:55:42.38 delay 1 second @18:55:43.39 AFV.select :holdingCoil @18:55:43.50 delay 1 second @18:55:44.53 AS.reconfigure ASfast @18:55:44.58 AS.seek 0ml @18:56:11.94 delay 3 seconds @18:56:14.96 AFV.select :carrierSolution @18:56:15.07 delay 2 seconds @18:56:17.10 AS.reconfigure ASconfig @18:56:17.15 AS.seek 1ml @18:57:14.99 delay 1 second @18:57:16.00 AFV.select :holdingCoil @18:57:16.14 delay 1 second @18:57:17.17 AS.reconfigure ASnorm @18:57:17.22 AS.seek 0ml @18:58:07.95 Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0418h1500ml.pcr @18:58:07.97 Building PCR Slug from ASV with MM2 mix, PP3 primer @18:58:07.98 Priming with carrier @18:58:07.99 ATV.dial ARV @18:58:08.57 ARV.dial :carrier @18:58:08.92 AS.seek 0.5ml @18:58:34.51 AFV.select :carrierSolution @18:58:34.63 delay 2 seconds @18:58:36.66 AS.reconfigure ASconfig @18:58:36.71 AS.seek 1ml @18:59:05.75 delay 1 second @18:59:06.77 AFV.select :holdingCoil @18:59:06.88 delay 1 second @18:59:07.90 ATV.dial :waste @18:59:09.43 AS.reconfigure ASnorm @18:59:09.48 AS.seek :empty @19:00:00.20 AFV.select :carrierSolution @19:00:00.32 delay 2 seconds @19:00:02.35 AS.reconfigure ASconfig @19:00:02.40 AS.seek 0.5ml @19:00:31.51 delay 1 second @19:00:32.52 AFV.select :holdingCoil @19:00:32.63 Building Slug @19:00:32.64 ATV.dial :ARV @19:00:34.13 delay 2 seconds @19:00:36.15 ARV.dial :air1 @19:00:36.53 delay 2 seconds @19:00:38.62 AS.reconfigure ASslow2 @19:00:38.67 AS.seek 0.506ml @19:00:45.04 delay 2 seconds @19:00:47.06 ARV.dial :MM2 @19:00:48.46 delay 2 seconds @19:00:50.49 Adding MM2 @19:00:50.57 AS.seek 0.524ml @19:01:08.91 delay 25 seconds @19:01:33.93 ARV.dial :PP3 @19:01:34.32 Adding PP3 @19:01:34.33 delay 2 seconds @19:01:36.42 AS.seek 0.53ml @19:01:42.61 delay 10 seconds @19:01:52.63 ARV.dial :air2 @19:01:53.02 adding ARV air @19:01:53.03 delay 2 seconds @19:01:55.12 AS.seek 0.536ml @19:02:01.52 delay 5 seconds @19:02:06.54 ARV.dial :carrier @19:02:07.74 delay 7 seconds @19:02:14.83 AS.seek 0.594ml @19:03:13.65 delay 5 seconds @19:03:18.67 ATV.dial :ASV @19:03:19.07 delay 2 seconds @19:03:21.16 AS.seek 0.6ml @19:03:27.35 delay 4 seconds @19:03:31.37 ATV.dial ARV @19:03:31.90 delay 2 seconds @19:03:33.92 ARV.dial :carrier @19:03:33.94 delay 2 seconds @19:03:36.03 AS.seek 0.65ml @19:04:26.52 delay 4 seconds @19:04:30.54 ATV.dial PCR @19:04:31.12 ASV.dial :waste1 @19:04:31.13 ARV.dial :air1 @19:04:31.53 Positioning Slug by 0.324ml @19:04:31.62 AS.seek 0.326ml @19:09:57.85 delay 5 seconds @19:10:02.88 ATV.dial ARV @19:10:03.60 ASV.dial :PCRlock @19:10:03.98 delay 5 seconds @19:10:09.01 Running Rp Profile60C300 thermocycling @19:10:09.02 PCR.program Profile60C300 @19:10:09.16 Profile60C300 should complete in < 1:45:21 @19:10:09.17 PCR.run 2:16:41.25 @20:32:06.49 PCR.off @20:32:08.05 PCR for Rp completed -- closed /var/log/esp/Rp18oct0418h1500ml.pcr @20:32:08.12 Cleaning PCR @20:32:08.13 AFV.select :carrierSolution @20:32:08.24 delay 2 seconds @20:32:10.27 AS.reconfigure ASconfig @20:32:10.32 AS.seek 0.5ml @20:32:20.61 delay 1 second @20:32:21.62 AFV.select :holdingCoil @20:32:21.73 ASV.dial :waste1 @20:32:22.09 ATV.dial :air3 @20:32:23.28 AS.reconfigure ASslow1 @20:32:23.34 AS.seek 0.51ml @20:32:25.66 delay 2 seconds @20:32:27.68 ATV.dial :bleach @20:32:28.87 AS.seek 0.81ml @20:33:29.48 delay 3 seconds @20:33:32.50 ATV.dial :air3 @20:33:33.03 delay 3 seconds @20:33:36.12 AS.seek 0.835ml @20:33:41.42 delay 3 seconds @20:33:44.44 ATV.dial :bleach @20:33:45.04 AS.seek 0.82ml @20:33:48.36 delay 3 seconds @20:33:51.38 ARV.dial :waste @20:33:51.97 delay 3 seconds @20:33:54.99 ATV.dial :ARV @20:33:55.93 AS.reconfigure ASconfig @20:33:55.98 AS.seek 0.47ml @20:34:16.38 AFV.select :carrierSolution @20:34:16.50 delay 2 seconds @20:34:18.53 AS.seek 1ml @20:34:49.28 delay 1 second @20:34:50.29 AFV.select :holdingCoil @20:34:50.40 delay 1 second @20:34:51.43 AS.reconfigure ASfast @20:34:51.48 AS.seek 0ml @20:35:18.79 AFV.select :carrierSolution @20:35:18.91 delay 2 seconds @20:35:20.94 AS.reconfigure ASconfig @20:35:20.99 AS.seek 1ml @20:36:18.72 delay 1 second @20:36:19.73 AFV.select :holdingCoil @20:36:19.84 delay 1 second @20:36:20.87 AS.reconfigure ASfast @20:36:20.92 AS.seek 0ml @20:36:48.24 AFV.select :carrierSolution @20:36:48.36 delay 2 seconds @20:36:50.39 AS.reconfigure ASconfig @20:36:50.44 AS.seek 1ml @20:37:48.25 delay 1 second @20:37:49.26 AFV.select :holdingCoil @20:37:49.37 delay 1 second @20:37:50.40 AS.reconfigure ASfast @20:37:50.45 AS.seek 0ml @20:38:17.77 ARV.dial between carrier and air1 @20:38:18.25 ATV.dial :air3 @20:38:19.45 AS.reconfigure ASslow1 @20:38:19.50 AS.seek 0.01ml @20:38:21.81 delay 2 seconds @20:38:23.82 ATV.dial :bleach @20:38:24.42 AS.seek 0.81ml @20:41:05.56 delay 3 seconds @20:41:08.58 ATV.dial :air3 @20:41:09.11 delay 3 seconds @20:41:12.20 AS.seek 0.835ml @20:41:17.42 delay 3 seconds @20:41:20.44 ATV.dial :bleach @20:41:21.04 AS.seek 0.82ml @20:41:24.33 delay 3 seconds @20:41:27.35 ATV.dial PCR @20:41:27.74 AS.reconfigure ASconfig @20:41:27.79 AS.seek :empty @20:42:15.24 PCR.program :bleachProfile @20:42:15.30 PCR program should complete in < 2:45 @20:42:15.31 PCR.run 10:00 @20:45:23.19 PCR.off @20:45:23.21 AFV.select :carrierSolution @20:45:23.32 delay 2 seconds @20:45:25.35 AS.seek 1ml @20:46:23.18 delay 1 second @20:46:24.19 AFV.select :holdingCoil @20:46:24.30 delay 1 second @20:46:25.33 AS.reconfigure ASfast @20:46:25.38 AS.seek 0ml @20:46:52.72 delay 3 seconds @20:46:55.74 AFV.select :carrierSolution @20:46:55.85 delay 2 seconds @20:46:57.88 AS.reconfigure ASconfig @20:46:57.94 AS.seek 1ml @20:47:55.72 delay 1 second @20:47:56.73 AFV.select :holdingCoil @20:47:56.84 delay 1 second @20:47:57.87 AS.reconfigure ASfast @20:47:57.92 AS.seek 0ml @20:48:25.25 delay 3 seconds @20:48:28.27 AFV.select :carrierSolution @20:48:28.38 delay 2 seconds @20:48:30.41 AS.reconfigure ASconfig @20:48:30.47 AS.seek 1ml @20:49:28.22 delay 1 second @20:49:29.23 AFV.select :holdingCoil @20:49:29.34 delay 1 second @20:49:30.37 AS.reconfigure ASfast @20:49:30.42 AS.seek 0ml @20:49:57.75 delay 3 seconds @20:50:00.77 AFV.select :carrierSolution @20:50:00.88 delay 2 seconds @20:50:03.38 AS.reconfigure ASconfig @20:50:03.51 AS.seek 1ml @20:51:01.29 delay 1 second @20:51:02.30 AFV.select :holdingCoil @20:51:02.41 delay 1 second @20:51:03.44 AS.reconfigure ASfast @20:51:03.49 AS.seek 0ml @20:51:30.83 delay 3 seconds @20:51:33.85 AFV.select :carrierSolution @20:51:33.96 delay 2 seconds @20:51:35.99 AS.reconfigure ASconfig @20:51:36.04 AS.seek 1ml @20:52:33.79 delay 1 second @20:52:34.80 AFV.select :holdingCoil @20:52:34.91 delay 1 second @20:52:35.94 AS.reconfigure ASnorm @20:52:35.99 AS.seek 0ml @20:53:26.71 Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0420h1500ml.pcr @20:53:26.73 Building PCR Slug from ASV with MM3 mix, PP5 primer @20:53:26.74 Priming with carrier @20:53:26.75 ATV.dial ARV @20:53:27.32 ARV.dial :carrier @20:53:27.67 AS.seek 0.5ml @20:53:53.18 AFV.select :carrierSolution @20:53:53.30 delay 2 seconds @20:53:55.33 AS.reconfigure ASconfig @20:53:55.38 AS.seek 1ml @20:54:24.44 delay 1 second @20:54:25.45 AFV.select :holdingCoil @20:54:25.56 delay 1 second @20:54:26.58 ATV.dial :waste @20:54:28.12 AS.reconfigure ASnorm @20:54:28.17 AS.seek :empty @20:55:18.90 AFV.select :carrierSolution @20:55:19.02 delay 2 seconds @20:55:21.05 AS.reconfigure ASconfig @20:55:21.10 AS.seek 0.5ml @20:55:50.21 delay 1 second @20:55:51.22 AFV.select :holdingCoil @20:55:51.33 Building Slug @20:55:51.34 ATV.dial :ARV @20:55:52.94 delay 2 seconds @20:55:54.96 ARV.dial :air1 @20:55:55.34 delay 2 seconds @20:55:57.42 AS.reconfigure ASslow2 @20:55:57.47 AS.seek 0.506ml @20:56:03.98 delay 2 seconds @20:56:06.00 ARV.dial :MM3 @20:56:06.99 delay 2 seconds @20:56:09.02 Adding MM3 @20:56:09.10 AS.seek 0.524ml @20:56:27.43 delay 25 seconds @20:56:52.45 ARV.dial :PP5 @20:56:52.82 Adding PP5 @20:56:52.83 delay 2 seconds @20:56:54.92 AS.seek 0.53ml @20:57:01.19 delay 10 seconds @20:57:11.21 ARV.dial :air3 @20:57:11.58 adding ARV air @20:57:11.59 delay 2 seconds @20:57:13.67 AS.seek 0.536ml @20:57:19.90 delay 5 seconds @20:57:24.92 ARV.dial :carrier @20:57:26.10 delay 7 seconds @20:57:33.19 AS.seek 0.594ml @20:58:31.77 delay 5 seconds @20:58:36.79 ATV.dial :ASV @20:58:37.19 delay 2 seconds @20:58:39.28 AS.seek 0.6ml @20:58:45.87 delay 4 seconds @20:58:49.89 ATV.dial ARV @20:58:50.51 delay 2 seconds @20:58:52.53 ARV.dial :carrier @20:58:52.55 delay 2 seconds @20:58:54.64 AS.seek 0.65ml @20:59:45.18 delay 4 seconds @20:59:49.20 ATV.dial PCR @20:59:49.79 ASV.dial :waste1 @20:59:49.80 ARV.dial :air1 @20:59:50.21 Positioning Slug by 0.324ml @20:59:50.30 AS.seek 0.326ml @21:05:16.57 delay 5 seconds @21:05:21.59 ATV.dial ARV @21:05:22.43 ASV.dial :PCRlock @21:05:22.81 delay 5 seconds @21:05:27.84 Running Bb Profile60C300 thermocycling @21:05:27.85 PCR.program Profile60C300 @21:05:27.99 Profile60C300 should complete in < 1:45:21 @21:05:28.00 PCR.run 2:16:41.25 @22:27:16.71 PCR.off @22:27:18.27 PCR for Bb completed -- closed /var/log/esp/Bb18oct0420h1500ml.pcr @22:27:18.34 Cleaning PCR @22:27:18.35 AFV.select :carrierSolution @22:27:18.46 delay 2 seconds @22:27:20.49 AS.reconfigure ASconfig @22:27:20.54 AS.seek 0.5ml @22:27:30.83 delay 1 second @22:27:31.84 AFV.select :holdingCoil @22:27:31.95 ASV.dial :waste1 @22:27:32.31 ATV.dial :air3 @22:27:33.48 AS.reconfigure ASslow1 @22:27:33.53 AS.seek 0.51ml @22:27:35.81 delay 2 seconds @22:27:37.83 ATV.dial :bleach @22:27:39.02 AS.seek 0.81ml @22:28:39.60 delay 3 seconds @22:28:42.62 ATV.dial :air3 @22:28:43.16 delay 3 seconds @22:28:46.25 AS.seek 0.835ml @22:28:51.58 delay 3 seconds @22:28:54.60 ATV.dial :bleach @22:28:55.20 AS.seek 0.82ml @22:28:58.50 delay 3 seconds @22:29:01.53 ARV.dial :waste @22:29:02.11 delay 3 seconds @22:29:05.13 ATV.dial :ARV @22:29:06.07 AS.reconfigure ASconfig @22:29:06.12 AS.seek 0.47ml @22:29:26.52 AFV.select :carrierSolution @22:29:26.64 delay 2 seconds @22:29:28.67 AS.seek 1ml @22:29:59.42 delay 1 second @22:30:00.43 AFV.select :holdingCoil @22:30:00.54 delay 1 second @22:30:01.57 AS.reconfigure ASfast @22:30:01.62 AS.seek 0ml @22:30:28.93 AFV.select :carrierSolution @22:30:29.05 delay 2 seconds @22:30:31.08 AS.reconfigure ASconfig @22:30:31.13 AS.seek 1ml @22:31:28.96 delay 1 second @22:31:29.97 AFV.select :holdingCoil @22:31:30.08 delay 1 second @22:31:31.11 AS.reconfigure ASfast @22:31:31.16 AS.seek 0ml @22:31:58.51 AFV.select :carrierSolution @22:31:58.63 delay 2 seconds @22:32:00.66 AS.reconfigure ASconfig @22:32:00.71 AS.seek 1ml @22:32:58.50 delay 1 second @22:32:59.51 AFV.select :holdingCoil @22:32:59.62 delay 1 second @22:33:00.65 AS.reconfigure ASfast @22:33:00.70 AS.seek 0ml @22:33:28.02 ARV.dial between carrier and air1 @22:33:28.50 ATV.dial :air3 @22:33:29.70 AS.reconfigure ASslow1 @22:33:29.75 AS.seek 0.01ml @22:33:32.08 delay 2 seconds @22:33:34.09 ATV.dial :bleach @22:33:34.70 AS.seek 0.81ml @22:36:15.80 delay 3 seconds @22:36:18.82 ATV.dial :air3 @22:36:19.35 delay 3 seconds @22:36:22.43 AS.seek 0.835ml @22:36:27.70 delay 3 seconds @22:36:30.72 ATV.dial :bleach @22:36:31.32 AS.seek 0.82ml @22:36:34.63 delay 3 seconds @22:36:37.65 ATV.dial PCR @22:36:38.04 AS.reconfigure ASconfig @22:36:38.09 AS.seek :empty @22:37:25.54 PCR.program :bleachProfile @22:37:25.60 PCR program should complete in < 2:45 @22:37:25.61 PCR.run 10:00 @22:40:33.49 PCR.off @22:40:33.51 AFV.select :carrierSolution @22:40:33.63 delay 2 seconds @22:40:35.66 AS.seek 1ml @22:41:33.49 delay 1 second @22:41:34.50 AFV.select :holdingCoil @22:41:34.61 delay 1 second @22:41:35.64 AS.reconfigure ASfast @22:41:35.69 AS.seek 0ml @22:42:03.04 delay 3 seconds @22:42:06.06 AFV.select :carrierSolution @22:42:06.17 delay 2 seconds @22:42:08.20 AS.reconfigure ASconfig @22:42:08.25 AS.seek 1ml @22:43:06.01 delay 1 second @22:43:07.02 AFV.select :holdingCoil @22:43:07.13 delay 1 second @22:43:08.16 AS.reconfigure ASfast @22:43:08.21 AS.seek 0ml @22:43:35.55 delay 3 seconds @22:43:38.57 AFV.select :carrierSolution @22:43:38.68 delay 2 seconds @22:43:40.71 AS.reconfigure ASconfig @22:43:40.77 AS.seek 1ml @22:44:38.55 delay 1 second @22:44:39.56 AFV.select :holdingCoil @22:44:39.67 delay 1 second @22:44:40.70 AS.reconfigure ASfast @22:44:40.75 AS.seek 0ml @22:45:08.08 delay 3 seconds @22:45:11.10 AFV.select :carrierSolution @22:45:11.21 delay 2 seconds @22:45:13.24 AS.reconfigure ASconfig @22:45:13.30 AS.seek 1ml @22:46:11.68 delay 1 second @22:46:12.69 AFV.select :holdingCoil @22:46:12.80 delay 1 second @22:46:13.83 AS.reconfigure ASfast @22:46:13.88 AS.seek 0ml @22:46:41.23 delay 3 seconds @22:46:44.25 AFV.select :carrierSolution @22:46:44.36 delay 2 seconds @22:46:46.39 AS.reconfigure ASconfig @22:46:46.46 AS.seek 1ml @22:47:44.24 delay 1 second @22:47:45.25 AFV.select :holdingCoil @22:47:45.36 delay 1 second @22:47:46.39 AS.reconfigure ASnorm @22:47:46.44 AS.seek 0ml @22:48:37.17 Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0422h1500ml.pcr @22:48:37.19 Building PCR Slug from ASV with MM1 mix, PP2 primer @22:48:37.20 Priming with carrier @22:48:37.21 ATV.dial ARV @22:48:37.79 ARV.dial :carrier @22:48:38.14 AS.seek 0.5ml @22:49:03.70 AFV.select :carrierSolution @22:49:03.82 delay 2 seconds @22:49:05.85 AS.reconfigure ASconfig @22:49:05.90 AS.seek 1ml @22:49:34.91 delay 1 second @22:49:35.92 AFV.select :holdingCoil @22:49:36.03 delay 1 second @22:49:37.05 ATV.dial :waste @22:49:38.59 AS.reconfigure ASnorm @22:49:38.64 AS.seek :empty @22:50:29.39 AFV.select :carrierSolution @22:50:29.51 delay 2 seconds @22:50:31.54 AS.reconfigure ASconfig @22:50:31.59 AS.seek 0.5ml @22:51:00.66 delay 1 second @22:51:01.67 AFV.select :holdingCoil @22:51:01.78 Building Slug @22:51:01.79 ATV.dial :ARV @22:51:03.40 delay 2 seconds @22:51:05.42 ARV.dial :air1 @22:51:05.80 delay 2 seconds @22:51:07.88 AS.reconfigure ASslow2 @22:51:07.94 AS.seek 0.506ml @22:51:14.24 delay 2 seconds @22:51:16.26 ARV.dial :MM1 @22:51:16.84 delay 2 seconds @22:51:18.87 Adding MM1 @22:51:18.94 AS.seek 0.524ml @22:51:37.60 delay 25 seconds @22:52:02.63 ARV.dial :PP2 @22:52:02.99 Adding PP2 @22:52:03.00 delay 2 seconds @22:52:05.09 AS.seek 0.53ml @22:52:11.32 delay 10 seconds @22:52:21.34 ARV.dial :air2 @22:52:21.71 adding ARV air @22:52:21.72 delay 2 seconds @22:52:23.81 AS.seek 0.536ml @22:52:30.20 delay 5 seconds @22:52:35.22 ARV.dial :carrier @22:52:36.41 delay 7 seconds @22:52:43.50 AS.seek 0.594ml @22:53:42.07 delay 5 seconds @22:53:47.10 ATV.dial :ASV @22:53:47.49 delay 2 seconds @22:53:49.58 AS.seek 0.6ml @22:53:55.73 delay 4 seconds @22:53:59.75 ATV.dial ARV @22:54:00.37 delay 2 seconds @22:54:02.40 ARV.dial :carrier @22:54:02.41 delay 2 seconds @22:54:04.50 AS.seek 0.65ml @22:54:55.06 delay 4 seconds @22:54:59.08 ATV.dial PCR @22:54:59.68 ASV.dial :waste1 @22:54:59.69 ARV.dial :air1 @22:55:00.08 Positioning Slug by 0.324ml @22:55:00.17 AS.seek 0.326ml @23:00:26.06 delay 5 seconds @23:00:31.08 ATV.dial ARV @23:00:31.91 ASV.dial :PCRlock @23:00:32.29 delay 5 seconds @23:00:37.32 Running Ss Profile60C350 thermocycling @23:00:37.33 PCR.program Profile60C350 @23:00:37.48 Profile60C350 should complete in < 1:48:05 @23:00:37.49 PCR.run 2:20:06.25 @00:23:00.04CEST05-Oct-18 PCR.off @00:23:02.09 PCR for Ss completed -- closed /var/log/esp/Ss18oct0422h1500ml.pcr @00:23:02.16 Cleaning PCR @00:23:02.74 AFV.select :carrierSolution @00:23:02.86 delay 2 seconds @00:23:04.89 AS.reconfigure ASconfig @00:23:04.94 AS.seek 0.5ml @00:23:15.27 delay 1 second @00:23:16.28 AFV.select :holdingCoil @00:23:16.39 ASV.dial :waste1 @00:23:16.75 ATV.dial :air3 @00:23:17.93 AS.reconfigure ASslow1 @00:23:17.98 AS.seek 0.51ml @00:23:20.24 delay 2 seconds @00:23:22.25 ATV.dial :bleach @00:23:22.86 AS.seek 0.81ml @00:24:23.46 delay 3 seconds @00:24:26.48 ATV.dial :air3 @00:24:27.02 delay 3 seconds @00:24:30.11 AS.seek 0.835ml @00:24:35.44 delay 3 seconds @00:24:38.46 ATV.dial :bleach @00:24:39.06 AS.seek 0.82ml @00:24:42.30 delay 3 seconds @00:24:45.32 ARV.dial :waste @00:24:45.91 delay 3 seconds @00:24:48.93 ATV.dial :ARV @00:24:49.77 AS.reconfigure ASconfig @00:24:49.83 AS.seek 0.47ml @00:25:10.21 AFV.select :carrierSolution @00:25:10.33 delay 2 seconds @00:25:12.36 AS.seek 1ml @00:25:43.11 delay 1 second @00:25:44.13 AFV.select :holdingCoil @00:25:44.24 delay 1 second @00:25:45.27 AS.reconfigure ASfast @00:25:45.32 AS.seek 0ml @00:26:12.65 AFV.select :carrierSolution @00:26:12.77 delay 2 seconds @00:26:14.80 AS.reconfigure ASconfig @00:26:14.85 AS.seek 1ml @00:27:12.63 delay 1 second @00:27:13.64 AFV.select :holdingCoil @00:27:13.75 delay 1 second @00:27:14.78 AS.reconfigure ASfast @00:27:14.83 AS.seek 0ml @00:27:42.15 AFV.select :carrierSolution @00:27:42.27 delay 2 seconds @00:27:44.30 AS.reconfigure ASconfig @00:27:44.35 AS.seek 1ml @00:28:42.10 delay 1 second @00:28:43.11 AFV.select :holdingCoil @00:28:43.22 delay 1 second @00:28:44.25 AS.reconfigure ASfast @00:28:44.30 AS.seek 0ml @00:29:11.65 ARV.dial between carrier and air1 @00:29:12.13 ATV.dial :air3 @00:29:13.34 AS.reconfigure ASslow1 @00:29:13.39 AS.seek 0.01ml @00:29:15.68 delay 2 seconds @00:29:17.69 ATV.dial :bleach @00:29:18.29 AS.seek 0.81ml @00:31:59.52 delay 3 seconds @00:32:02.54 ATV.dial :air3 @00:32:03.07 delay 3 seconds @00:32:06.16 AS.seek 0.835ml @00:32:11.45 delay 3 seconds @00:32:14.47 ATV.dial :bleach @00:32:15.08 AS.seek 0.82ml @00:32:18.38 delay 3 seconds @00:32:21.40 ATV.dial PCR @00:32:21.79 AS.reconfigure ASconfig @00:32:21.84 AS.seek :empty @00:33:09.29 PCR.program :bleachProfile @00:33:09.36 PCR program should complete in < 2:45 @00:33:09.37 PCR.run 10:00 @00:36:17.25 PCR.off @00:36:17.28 AFV.select :carrierSolution @00:36:17.39 delay 2 seconds @00:36:19.41 AS.seek 1ml @00:37:17.31 delay 1 second @00:37:18.32 AFV.select :holdingCoil @00:37:18.43 delay 1 second @00:37:19.46 AS.reconfigure ASfast @00:37:19.51 AS.seek 0ml @00:37:46.85 delay 3 seconds @00:37:49.87 AFV.select :carrierSolution @00:37:49.98 delay 2 seconds @00:37:52.01 AS.reconfigure ASconfig @00:37:52.06 AS.seek 1ml @00:38:49.82 delay 1 second @00:38:50.83 AFV.select :holdingCoil @00:38:51.45 delay 1 second @00:38:52.56 AS.reconfigure ASfast @00:38:52.61 AS.seek 0ml @00:39:19.96 delay 3 seconds @00:39:22.98 AFV.select :carrierSolution @00:39:23.09 delay 2 seconds @00:39:25.12 AS.reconfigure ASconfig @00:39:25.18 AS.seek 1ml @00:40:22.98 delay 1 second @00:40:23.99 AFV.select :holdingCoil @00:40:24.10 delay 1 second @00:40:25.13 AS.reconfigure ASfast @00:40:25.18 AS.seek 0ml @00:40:52.54 delay 3 seconds @00:40:55.56 AFV.select :carrierSolution @00:40:55.67 delay 2 seconds @00:40:57.70 AS.reconfigure ASconfig @00:40:57.76 AS.seek 1ml @00:41:55.63 delay 1 second @00:41:56.64 AFV.select :holdingCoil @00:41:56.75 delay 1 second @00:41:57.78 AS.reconfigure ASfast @00:41:57.83 AS.seek 0ml @00:42:25.18 delay 3 seconds @00:42:28.20 AFV.select :carrierSolution @00:42:28.31 delay 2 seconds @00:42:30.34 AS.reconfigure ASconfig @00:42:30.41 AS.seek 1ml @00:43:28.18 delay 1 second @00:43:29.19 AFV.select :holdingCoil @00:43:29.30 delay 1 second @00:43:30.33 AS.reconfigure ASnorm @00:43:30.38 AS.seek 0ml @00:44:21.07 Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0500h1500ml.pcr @00:44:21.09 Building PCR Slug from ASV with MM1 mix, PP1 primer @00:44:21.11 Priming with carrier @00:44:21.12 ATV.dial ARV @00:44:21.70 ARV.dial :carrier @00:44:22.08 AS.seek 0.5ml @00:44:47.59 AFV.select :carrierSolution @00:44:47.71 delay 2 seconds @00:44:49.74 AS.reconfigure ASconfig @00:44:49.79 AS.seek 1ml @00:45:18.87 delay 1 second @00:45:19.89 AFV.select :holdingCoil @00:45:20.00 delay 1 second @00:45:21.03 ATV.dial :waste @00:45:22.56 AS.reconfigure ASnorm @00:45:22.61 AS.seek :empty @00:46:13.34 AFV.select :carrierSolution @00:46:13.46 delay 2 seconds @00:46:15.49 AS.reconfigure ASconfig @00:46:15.54 AS.seek 0.5ml @00:46:44.65 delay 1 second @00:46:45.66 AFV.select :holdingCoil @00:46:45.77 Building Slug @00:46:45.78 ATV.dial :ARV @00:46:47.28 delay 2 seconds @00:46:49.30 ARV.dial :air1 @00:46:49.79 delay 2 seconds @00:46:51.88 AS.reconfigure ASslow2 @00:46:51.93 AS.seek 0.506ml @00:46:58.39 delay 2 seconds @00:47:00.41 ARV.dial :MM1 @00:47:00.98 delay 2 seconds @00:47:03.01 Adding MM1 @00:47:03.08 AS.seek 0.524ml @00:47:21.39 delay 25 seconds @00:47:46.41 ARV.dial :PP1 @00:47:46.78 Adding PP1 @00:47:46.79 delay 2 seconds @00:47:48.88 AS.seek 0.53ml @00:47:55.09 delay 10 seconds @00:48:05.11 ARV.dial :air1 @00:48:05.48 adding ARV air @00:48:05.49 delay 2 seconds @00:48:07.58 AS.seek 0.536ml @00:48:14.03 delay 5 seconds @00:48:19.05 ARV.dial :carrier @00:48:19.45 delay 7 seconds @00:48:26.54 AS.seek 0.594ml @00:49:25.04 delay 5 seconds @00:49:30.07 ATV.dial :ASV @00:49:30.47 delay 2 seconds @00:49:32.56 AS.seek 0.6ml @00:49:38.87 delay 4 seconds @00:49:42.89 ATV.dial ARV @00:49:43.42 delay 2 seconds @00:49:45.44 ARV.dial :carrier @00:49:45.46 delay 2 seconds @00:49:47.55 AS.seek 0.65ml @00:50:38.48 delay 4 seconds @00:50:42.51 ATV.dial PCR @00:50:43.08 ASV.dial :waste1 @00:50:43.09 ARV.dial :air1 @00:50:43.49 Positioning Slug by 0.324ml @00:50:43.58 AS.seek 0.326ml @00:56:09.89 delay 5 seconds @00:56:14.91 ATV.dial ARV @00:56:15.63 ASV.dial :PCRlock @00:56:16.01 delay 5 seconds @00:56:21.04 Running IPc ProfileIPc thermocycling @00:56:21.65 PCR.program ProfileIPc @00:56:21.80 ProfileIPc should complete in < 1:50:49 @00:56:21.81 PCR.run 2:23:31.25 @02:20:31.14 PCR.off @02:20:33.79 PCR for IPc completed -- closed /var/log/esp/IPc18oct0500h1500ml.pcr @02:20:33.81 Full MFB Decontamination @02:20:33.82 Decon extraction column and decon AIV @02:20:33.84 SPE.hold 2:46:40,55C @02:20:33.89 ASV.dial :SPEdelivery @02:20:34.46 delay 20 seconds @02:20:54.49 AFV.select :carrierSolution @02:20:54.60 delay 2 seconds @02:20:56.63 AS.reconfigure ASconfig @02:20:56.69 AS.seek 0.5ml @02:21:06.96 delay 1 second @02:21:07.98 AFV.select :holdingCoil @02:21:08.09 ATV.dial :air1 @02:21:09.75 AS.reconfigure ASslow1 @02:21:09.80 AS.seek 0.52ml @02:21:14.06 ATV.dial :ASV @02:21:15.60 ASV.dial :SPErecovery @02:21:16.24 AS.seek 0.62ml @02:21:36.62 ATV.dial :waste @02:21:38.34 AS.reconfigure ASnorm @02:21:38.39 AS.seek :empty @02:22:10.01 AFV.select :holdingCoil @02:22:10.03 ATV.dial :waste @02:22:10.05 AS.reconfigure ASconfig @02:22:10.10 AS.seek 0ml @02:22:10.42 AFV.select :carrierSolution @02:22:10.54 delay 2 seconds @02:22:12.57 AS.seek 0.5ml @02:22:41.59 delay 1 second @02:22:42.61 AFV.select :holdingCoil @02:22:42.72 ATV.dial :air3 @02:22:43.36 AS.reconfigure ASslow1 @02:22:43.41 AS.seek 0.52ml @02:22:47.72 ATV.dial :air3 @02:22:47.81 AS.seek 0.53ml @02:22:50.15 delay 2 seconds @02:22:52.16 ATV.dial :bleach @02:22:52.63 AS.seek 0.71ml @02:23:29.15 delay 3 seconds @02:23:32.17 ATV.dial :air3 @02:23:32.70 delay 3 seconds @02:23:35.79 AS.seek 0.735ml @02:23:41.10 delay 3 seconds @02:23:44.12 ATV.dial :bleach @02:23:44.72 AS.seek 0.72ml @02:23:48.04 delay 3 seconds @02:23:51.07 ATV.dial ASV @02:23:52.03 ASV.dial :SPErecovery @02:23:52.12 AS.seek 0.365ml @02:25:03.77 ATV.dial :waste @02:25:05.34 AS.reconfigure ASnorm @02:25:05.39 AS.seek :empty @02:25:24.16 ATV.dial :air3 @02:25:24.81 AS.reconfigure ASslow1 @02:25:24.86 AS.seek 0.009ml @02:25:27.12 delay 2 seconds @02:25:29.13 ATV.dial :bleach @02:25:29.58 AS.seek 0.579ml @02:27:24.49 delay 3 seconds @02:27:27.51 ATV.dial :air3 @02:27:28.04 delay 3 seconds @02:27:31.13 AS.seek 0.604ml @02:27:36.44 delay 3 seconds @02:27:39.46 ATV.dial :bleach @02:27:40.07 AS.seek 0.589ml @02:27:43.37 delay 3 seconds @02:27:46.39 ATV.dial :ASV @02:27:47.39 ASV.dial :wasteR @02:27:48.05 AS.seek 0ml @02:29:46.79 delay 1:00 @02:30:46.81 ATV.dial ASV @02:30:46.83 AFV.select :carrierSolution @02:30:46.94 delay 2 seconds @02:30:48.97 AS.reconfigure ASconfig @02:30:49.03 AS.seek 1ml @02:31:46.79 delay 1 second @02:31:47.81 AFV.select :holdingCoil @02:31:47.92 delay 1 second @02:31:48.94 ASV.dial :wasteR @02:31:48.96 AS.reconfigure ASslow1 @02:31:49.01 AS.seek 0ml @02:35:10.40 delay 10 seconds @02:35:20.42 ATV.dial :air3 @02:35:21.82 AS.seek 0.01ml @02:35:24.15 delay 2 seconds @02:35:26.16 ATV.dial :bleach @02:35:26.75 AS.seek 0.58ml @02:37:21.67 delay 3 seconds @02:37:24.69 ATV.dial :air3 @02:37:25.22 delay 3 seconds @02:37:28.31 AS.seek 0.605ml @02:37:33.68 delay 3 seconds @02:37:36.70 ATV.dial :bleach @02:37:37.30 AS.seek 0.59ml @02:37:40.61 delay 3 seconds @02:37:43.64 ATV.dial ASV @02:37:44.61 ASV.dial :SPEdelivery @02:37:44.98 AS.seek 0ml @02:39:43.90 delay 1:00 @02:40:43.92 AFV.select :carrierSolution @02:40:44.03 delay 2 seconds @02:40:46.06 AS.reconfigure ASconfig @02:40:46.11 AS.seek 1ml @02:41:43.94 delay 1 second @02:41:44.96 AFV.select :holdingCoil @02:41:45.07 delay 1 second @02:41:46.09 ATV.dial ASV @02:41:46.11 ASV.dial :SPEdelivery @02:41:46.13 AS.reconfigure ASslow1 @02:41:46.17 AS.seek 0ml @02:45:07.58 delay 10 seconds @02:45:17.60 ATV.dial :air2 @02:45:18.37 AS.reconfigure ASslow0 @02:45:18.43 AS.seek 0.8ml @02:46:39.23 delay 3 seconds @02:46:42.25 ATV.dial :ASV @02:46:43.17 ASV.dial :SPEdelivery @02:46:43.19 AS.reconfigure ASslow1 @02:46:43.24 AS.seek 0.03ml @02:49:18.36 delay 20 seconds @02:49:38.39 ATV.dial :waste,via: :air3 @02:49:39.97 AS.reconfigure ASconfig @02:49:40.02 AS.seek :empty @02:49:42.10 SPE.coast @02:49:42.19 Removing Bleach from Mixing Coil @02:49:42.20 AFV.select :carrierSolution @02:49:42.31 delay 2 seconds @02:49:44.34 AS.seek 0.5ml @02:50:13.45 delay 1 second @02:50:14.46 AFV.select :holdingCoil @02:50:14.57 ATV.dial :ASV @02:50:16.24 ASV.dial :SPErecovery @02:50:16.87 AS.reconfigure ASslow1 @02:50:16.92 AS.seek 0.73ml @02:51:03.43 ASV.dial :wasteD @02:51:04.09 AS.reconfigure ASnorm @02:51:04.14 AS.seek 0ml @02:51:41.24 AFV.select :carrierSolution @02:51:41.36 delay 2 seconds @02:51:43.39 AS.reconfigure ASconfig @02:51:43.44 AS.seek 0.5ml @02:52:12.45 delay 1 second @02:52:13.46 AFV.select :holdingCoil @02:52:13.57 ATV.dial ASV @02:52:13.58 ASV.dial :SPErecovery @02:52:14.20 AS.reconfigure ASslow0 @02:52:14.25 AS.seek 0.3ml @02:52:34.68 ATV.dial :air2 @02:52:35.67 AS.reconfigure ASslow1 @02:52:35.72 AS.seek 0.32ml @02:52:40.02 ATV.dial ASV @02:52:41.01 AS.reconfigure ASslow0 @02:52:41.06 AS.seek 0.57ml @02:53:06.46 ASV.dial :wasteD @02:53:07.13 AS.reconfigure ASnorm @02:53:07.19 AS.seek 0ml @02:53:36.26 AFV.select :carrierSolution @02:53:36.38 delay 2 seconds @02:53:38.41 AS.reconfigure ASconfig @02:53:38.46 AS.seek 0.5ml @02:54:07.55 delay 1 second @02:54:08.56 AFV.select :holdingCoil @02:54:08.67 ATV.dial ASV @02:54:08.68 ASV.dial :SPErecovery @02:54:09.88 AS.reconfigure ASslow0 @02:54:09.93 AS.seek 0.3ml @02:54:30.35 ATV.dial :air2 @02:54:31.34 AS.reconfigure ASslow1 @02:54:31.39 AS.seek 0.32ml @02:54:35.76 ATV.dial ASV @02:54:36.74 AS.reconfigure ASslow0 @02:54:36.79 AS.seek 0.57ml @02:55:02.24 ASV.dial :wasteD @02:55:02.89 AS.reconfigure ASnorm @02:55:02.95 AS.seek 0ml @02:55:32.02 ATV.dial :air2 @02:55:33.03 AS.reconfigure ASslow0 @02:55:33.09 AS.seek 0.3ml @02:56:03.63 ATV.dial :ASV @02:56:04.53 ASV.dial :wasteD @02:56:04.55 AS.reconfigure ASconfig @02:56:04.60 AS.seek 0.05ml @02:56:19.27 ATV.dial :waste @02:56:21.00 AS.seek 0ml @02:56:24.15 ASV.dial :SPEdelivery @02:56:25.11 deconLysateINT @02:56:25.13 ATV.dial :air3 @02:56:25.79 AS.reconfigure ASslow1 @02:56:25.84 AS.seek 0.01ml @02:56:28.22 delay 2 seconds @02:56:30.23 ATV.dial :bleach @02:56:30.70 AS.seek 0.96ml @02:59:42.05 delay 3 seconds @02:59:45.07 ATV.dial :air3 @02:59:45.60 delay 3 seconds @02:59:48.69 AS.seek 0.985ml @02:59:54.00 delay 3 seconds @02:59:57.02 ATV.dial :bleach @02:59:57.62 AS.seek 0.97ml @03:00:00.94 delay 3 seconds @03:00:03.97 ATV.dial AIV @03:00:05.13 AIV.dial :coilToMFB @03:00:05.14 AWV.select :waste @03:00:05.16 AFV.select :carrierSolution @03:00:05.27 delay 2 seconds @03:00:07.30 AS.reconfigure ASconfig @03:00:07.35 AS.seek 1ml @03:00:09.38 delay 1 second @03:00:10.39 AFV.select :holdingCoil @03:00:10.50 delay 1 second @03:00:11.53 AS.reconfigure ASslow0 @03:00:11.57 AS.seek :empty @03:01:52.52 AFV.select :carrierSolution @03:01:52.64 delay 2 seconds @03:01:54.67 AS.reconfigure ASconfig @03:01:54.72 AS.seek 1ml @03:02:52.52 delay 1 second @03:02:53.53 AFV.select :holdingCoil @03:02:53.64 delay 1 second @03:02:54.67 AS.reconfigure ASslow0 @03:02:54.72 AS.seek :empty @03:04:35.68 AFV.select :carrierSolution @03:04:35.80 delay 2 seconds @03:04:37.83 AS.reconfigure ASconfig @03:04:37.88 AS.seek 1ml @03:05:35.75 delay 1 second @03:05:36.76 AFV.select :holdingCoil @03:05:36.87 delay 1 second @03:05:37.90 AS.reconfigure ASslow0 @03:05:37.95 AS.seek :empty @03:07:18.93 ATV.dial :air2 @03:07:19.58 AS.reconfigure ASslow1 @03:07:19.63 AS.seek 0.009ml @03:07:21.91 AS.reconfigure ASslow0 @03:07:21.96 AS.seek 1ml @03:09:01.92 ATV.dial AIV @03:09:02.77 AS.seek 0.02ml @03:10:41.64 ATV.dial :waste @03:10:43.16 AS.seek 0ml @03:10:45.50 AWV.select :air @03:10:45.67 Cleaning PCR @03:10:45.68 AFV.select :carrierSolution @03:10:45.79 delay 2 seconds @03:10:47.82 AS.reconfigure ASconfig @03:10:47.87 AS.seek 0.5ml @03:11:16.90 delay 1 second @03:11:17.91 AFV.select :holdingCoil @03:11:18.02 ASV.dial :waste1 @03:11:18.38 ATV.dial :air3 @03:11:19.03 AS.reconfigure ASslow1 @03:11:19.08 AS.seek 0.51ml @03:11:21.38 delay 2 seconds @03:11:23.39 ATV.dial :bleach @03:11:24.45 AS.seek 0.81ml @03:12:25.10 delay 3 seconds @03:12:28.12 ATV.dial :air3 @03:12:28.65 delay 3 seconds @03:12:31.74 AS.seek 0.835ml @03:12:36.97 delay 3 seconds @03:12:39.99 ATV.dial :bleach @03:12:40.59 AS.seek 0.82ml @03:12:43.87 delay 3 seconds @03:12:46.89 ARV.dial :waste @03:12:47.49 delay 3 seconds @03:12:50.51 ATV.dial :ARV @03:12:51.45 AS.reconfigure ASconfig @03:12:51.50 AS.seek 0.47ml @03:13:11.90 AFV.select :carrierSolution @03:13:12.02 delay 2 seconds @03:13:14.05 AS.seek 1ml @03:13:44.85 delay 1 second @03:13:45.86 AFV.select :holdingCoil @03:13:45.97 delay 1 second @03:13:47.00 AS.reconfigure ASfast @03:13:47.05 AS.seek 0ml @03:14:14.39 AFV.select :carrierSolution @03:14:14.51 delay 2 seconds @03:14:16.54 AS.reconfigure ASconfig @03:14:16.59 AS.seek 1ml @03:15:14.43 delay 1 second @03:15:15.45 AFV.select :holdingCoil @03:15:15.56 delay 1 second @03:15:16.59 AS.reconfigure ASfast @03:15:16.64 AS.seek 0ml @03:15:43.98 AFV.select :carrierSolution @03:15:44.10 delay 2 seconds @03:15:46.13 AS.reconfigure ASconfig @03:15:46.18 AS.seek 1ml @03:16:44.02 delay 1 second @03:16:45.03 AFV.select :holdingCoil @03:16:45.14 delay 1 second @03:16:46.17 AS.reconfigure ASfast @03:16:46.22 AS.seek 0ml @03:17:13.57 ARV.dial between carrier and air1 @03:17:14.05 ATV.dial :air3 @03:17:15.25 AS.reconfigure ASslow1 @03:17:15.30 AS.seek 0.01ml @03:17:17.60 delay 2 seconds @03:17:19.61 ATV.dial :bleach @03:17:20.22 AS.seek 0.81ml @03:20:01.48 delay 3 seconds @03:20:04.50 ATV.dial :air3 @03:20:05.01 delay 3 seconds @03:20:08.10 AS.seek 0.835ml @03:20:13.44 delay 3 seconds @03:20:16.46 ATV.dial :bleach @03:20:17.06 AS.seek 0.82ml @03:20:20.38 delay 3 seconds @03:20:23.40 ATV.dial PCR @03:20:23.79 AS.reconfigure ASconfig @03:20:23.84 AS.seek :empty @03:21:11.30 PCR.program :bleachProfile @03:21:11.37 PCR program should complete in < 2:45 @03:21:11.38 PCR.run 10:00 @03:24:31.38 PCR.off @03:24:31.40 AFV.select :carrierSolution @03:24:31.51 delay 2 seconds @03:24:33.54 AS.seek 1ml @03:25:31.37 delay 1 second @03:25:32.38 AFV.select :holdingCoil @03:25:32.49 delay 1 second @03:25:33.52 AS.reconfigure ASfast @03:25:33.57 AS.seek 0ml @03:26:00.92 delay 3 seconds @03:26:03.94 AFV.select :carrierSolution @03:26:04.05 delay 2 seconds @03:26:06.08 AS.reconfigure ASconfig @03:26:06.13 AS.seek 1ml @03:27:03.87 delay 1 second @03:27:04.88 AFV.select :holdingCoil @03:27:04.99 delay 1 second @03:27:06.02 AS.reconfigure ASfast @03:27:06.07 AS.seek 0ml @03:27:33.42 delay 3 seconds @03:27:36.44 AFV.select :carrierSolution @03:27:36.55 delay 2 seconds @03:27:38.58 AS.reconfigure ASconfig @03:27:38.63 AS.seek 1ml @03:28:36.43 delay 1 second @03:28:37.44 AFV.select :holdingCoil @03:28:37.55 delay 1 second @03:28:38.58 AS.reconfigure ASfast @03:28:38.63 AS.seek 0ml @03:29:05.98 delay 3 seconds @03:29:09.00 AFV.select :carrierSolution @03:29:09.11 delay 2 seconds @03:29:11.69 AS.reconfigure ASconfig @03:29:11.78 AS.seek 1ml @03:30:09.53 delay 1 second @03:30:10.54 AFV.select :holdingCoil @03:30:10.65 delay 1 second @03:30:11.68 AS.reconfigure ASfast @03:30:11.73 AS.seek 0ml @03:30:39.06 delay 3 seconds @03:30:42.08 AFV.select :carrierSolution @03:30:42.19 delay 2 seconds @03:30:44.22 AS.reconfigure ASconfig @03:30:44.28 AS.seek 1ml @03:31:42.03 delay 1 second @03:31:43.04 AFV.select :holdingCoil @03:31:43.15 delay 1 second @03:31:44.18 AS.reconfigure ASnorm @03:31:44.23 AS.seek 0ml @03:32:34.94 AFV.select :carrierSolution @03:32:35.05 delay 2 seconds @03:32:37.08 AS.reconfigure ASconfig @03:32:37.13 AS.seek 1ml @03:33:34.92 delay 1 second @03:33:35.93 AFV.select :holdingCoil @03:33:36.04 delay 1 second @03:33:37.06 ATV.dial :waste @03:33:38.18 AS.reconfigure ASnorm @03:33:38.24 AS.seek 0.5ml @03:34:03.78 ATV.dial :waste2 @03:34:04.98 AS.seek 0ml @03:34:30.50 AS.coast @03:34:30.54 SS.coast @03:34:30.58 CC.coast @03:34:30.63 CS.coast @03:34:30.67 PC.coast @03:34:30.71 PS.coast @03:34:30.75 Forearm.coast @03:34:30.79 Elbow.coast @03:34:31.19 Gate.power :core,:OFF @03:34:31.21 Gate.power :raw,:OFF @03:34:31.22 Shutdown @03:34:31.23 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @13:10:55.88 Session Begun @13:20:59.13 -> exit @13:20:59.15 Session Ended @13:28:01.70 Session Begun @13:28:17.52 -> showlog 100 @13:38:34.22 -> exit @13:38:34.24 Session Ended @14:00:00.06
Powering core bus @14:00:05.09 Gate.power :raw,:ON @14:00:05.78 SPE.coast @14:00:05.84 SPE.configure SPEconfig @14:00:05.95 AS.configure ASconfig @14:00:06.05 SS.configure SSconfig @14:00:06.15 CC.configure CCconfig @14:00:06.25 CS.configure CSconfig @14:00:06.35 PC.configure PCconfig @14:00:06.44 PS.configure PSconfig @14:00:06.57 Forearm.configure LooseForearm @14:00:06.66 Elbow.configure LooseElbow @14:00:06.79 SC.configure SCloose @14:00:06.89 SE.configure SEconfig @14:00:06.94 CH.coast @14:00:07.00 CH.configure CHconfig @14:00:07.55 PH.coast @14:00:07.61 PH.configure PHconfig @14:00:07.65 PCR.off @14:00:07.69 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:07.72 PCR.caltemp= 2.7073,0.0373,0 @14:00:07.74 All dwarf controllers running firmware version 3.75 @14:00:07.87 Valve.reset @14:00:08.04 PCR.off @14:00:08.07 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:08.09 PCR.caltemp= 2.7073,0.0373,0 @14:00:08.32 Intake.select :close @14:00:08.34 Exhaust.select :close @14:00:08.35 CTV.dial :air @14:00:08.45 PSV.dial :air @14:00:08.49 CSV.dial :waste1 @14:00:08.65
Forearm.reconfigure LooseForearm @14:00:08.72 Forearm.home.seek :retract @14:00:08.73 SSV.dial :spit,avoiding: :exhaust @14:00:09.02 PS.reconfigure PSconfig @14:00:09.07 PS.home.seek 0ml @14:00:09.52 Hand.close @14:00:09.56
Elbow.jog 1500 @14:00:09.68 CS.reconfigure CSfast @14:00:09.71 SS.reconfigure SSconfig @14:00:09.82 CS.home.seek 0ml @14:00:09.83 SS.home.seek 0ml @14:00:10.28
Elbow.reconfigure LooseElbow @14:00:10.32 Elbow.home.seek :clear @14:00:13.70 SE.reconfigure SEconfig @14:00:13.75 SE.home.seek :down @14:00:14.49 SC.reconfigure SCsloppy @14:00:14.54 SC.home.seek :initial @14:00:16.74 Forearm.seek :retract @14:00:16.82 Elbow.seek :hammer @14:00:17.97 Forearm.reconfigure TightForearm @14:00:18.04 Forearm.seek 5 + 350 counts @14:00:18.07 PS.coast @14:00:19.24
Forearm.seek :retract @14:00:20.47 PC.reconfigure PCconfig @14:00:20.49
CC.reconfigure CCconfig @14:00:20.57 PC.home.seek :open @14:00:20.61
CC.home.seek :open @14:00:24.02 Phase [phasewithdil] @14:00:24.03 GoodNews.email "At 14:00:23 began in tube 2: phasewithdil\n/home/esp/esp2/mission/18tundeployv2dil.rb:118\n{1 => 15, 2 => 7}\nCan@14:00:24, 13.9C, 69% humidity, 14.3psia, 13.918V, 0.645A, 0.645A avg, 8.97W",Subject: "Started 18tundeployv2dil phase phasewithdil" @14:00:24.06CEST05-Oct-18
==================> STARTED Superphasewdil (phase #2) <=== @14:00:24.08 Starting initialPurge @14:00:24.10 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:00:24.17 Hand.open @14:00:24.96 Elbow.reconfigure TightElbow @14:00:25.01 Elbow.seek FlushPuck::Garage @14:00:26.98 Elbow.reconfigure LooseElbow @14:00:27.12 Forearm.reconfigure LooseForearm @14:00:27.17 Forearm.seek :garage @14:00:28.14 Hand.close @14:00:28.86 Forearm.seek :retract @14:00:29.89 Elbow.reconfigure TightElbow @14:00:29.94 Elbow.seek CC @14:00:32.89 Elbow.reconfigure LooseElbow @14:00:33.02 Forearm.reconfigure TightForearm @14:00:33.07 Forearm.seek CC @14:00:34.37 CC.seek :closed @14:00:38.61 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.84 @14:00:38.70 Hand.open @14:00:39.41 Forearm.reconfigure LooseForearm @14:00:39.48 Forearm.seek :retract @14:00:40.46 Flushing the Collection Valve::Manifold @14:00:40.59 CSV.dial CSR @14:00:41.98 Pulling 5ml of CSR.flush @14:00:41.99 CSR.select :flush @14:00:42.12 CS.reconfigure CSconfig @14:00:42.16 CS.seek 5ml @14:00:55.89 delay 5 seconds @14:01:00.91 CSR.select :air @14:01:01.08 CS.reconfigure CSfast @14:01:01.13 CS.seek 7.5ml @14:01:07.74 CSV.dial :waste @14:01:09.05 CS.reconfigure CSconfig @14:01:09.10 CS.seek 0ml @14:01:29.29 Purging external Intake @14:01:29.42 CSV.dial :flush @14:01:29.88 CS.reconfigure CSfast @14:01:29.93 CS.seek 10ml @14:01:53.10 delay 5 seconds @14:01:58.13 CSV.dial CBV @14:01:58.17
CBV.dial CSV @14:01:58.93 CTV.dial :intake @14:01:59.68 Intake.select :open @14:01:59.81 CS.seek 0ml @14:02:23.81 Intake.select :close @14:02:23.93 CTV.dial :air @14:02:24.69 Flushing upper manifold @14:02:24.82 CSV.dial :flush @14:02:25.57 CS.seek 5ml @14:02:38.29 delay 5 seconds @14:02:43.31 CSV.dial :air @14:02:43.86 CS.seek 7ml @14:02:49.32 CSV.dial CBV @14:02:49.36
CBV.dial CSV @14:02:50.40 CTV.dial :air @14:02:50.42 CS.seek 0ml @14:03:06.91 delay 7 seconds @14:03:13.95 CS.seek 3ml @14:03:21.72 CSV.dial :waste @14:03:22.19 CS.seek 0ml @14:03:29.94 CSV.dial :flush @14:03:30.41 CS.seek 3ml @14:03:38.19 delay 5 seconds @14:03:43.21 CSV.dial :air @14:03:43.76 CS.seek 5ml @14:03:49.22 CSV.dial :air @14:03:49.25 CS.seek 0ml @14:04:01.32 delay 7 seconds @14:04:08.35 CS.seek 3ml @14:04:16.00 CSV.dial :waste @14:04:16.78 CS.seek 0ml @14:04:24.48 Garaging FlushPuck in Collection Clamp @14:04:24.56 Elbow.reconfigure TightElbow @14:04:24.61 Elbow.seek CC @14:04:24.88 Elbow.reconfigure LooseElbow @14:04:24.99 Forearm.seek CC @14:04:25.98 Hand.close @14:04:26.59 CC.seek :open @14:04:30.95 Forearm.seek :retract @14:04:32.01 Elbow.reconfigure TightElbow @14:04:32.06 Elbow.seek :garage @14:04:35.08 Elbow.reconfigure LooseElbow @14:04:35.20 Forearm.reconfigure TightForearm @14:04:35.25 Forearm.seek :garage @14:04:36.40 Hand.open @14:04:37.16 Forearm.reconfigure LooseForearm @14:04:37.20 Forearm.seek :retract @14:04:38.29 Starting mfb @14:04:38.30 pcrBAC sampling at most 1500ml, wcr at most 1500ml @14:04:38.33 BACfans.email "Started mfb in tube 2 at 14:04:38CEST05-Oct-18\n{1 => 15, 2 => 7}\nCan@14:00:24, 13.9C, 69% humidity, 14.3psia, 13.918V, 0.645A, 0.645A avg, 8.97W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @14:04:38.41
Loading sh1 puck from tube 2 into Collection Clamp @14:04:38.54 Readying the MFB @14:04:38.58 Homing MFB Syringe @14:04:38.62 AFV.select :holdingCoil @14:04:38.86 ATV.dial :air2 @14:04:38.99
SC.reconfigure SCloose @14:04:39.23 Elbow.reconfigure TightElbow @14:04:39.28 Elbow.seek 2 @14:04:39.44 AS.reconfigure ASconfig @14:04:39.49 AS.home.seek :home @14:04:41.32
Forearm.reconfigure TightForearm @14:04:41.37 Forearm.seek 2 + 700 counts @14:04:42.41 SE.reconfigure SEpick @14:04:42.48 SE.seek beamBreak @14:04:44.93 SE.configure SEconfig @14:04:45.54 AFV.select :carrierSolution @14:04:45.75
Elbow.seek 2 @14:04:45.81 delay 2 seconds @14:04:46.09
Forearm.seek 2 + 300 counts @14:04:46.32 Forearm.reconfigure LooseForearm @14:04:46.37 Hand.close @14:04:47.02 SE.seek :down @14:04:47.29
Forearm.seek :retract @14:04:47.85 AS.seek 1ml @14:04:48.23
Elbow.seek CC @14:04:49.29 SE.coast @14:04:49.92
Elbow.reconfigure LooseElbow @14:04:50.03 Forearm.reconfigure TightForearm @14:04:50.08 Forearm.seek CC @14:04:51.38 CC.seek :closed @14:04:55.66 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.90 @14:04:55.67 Hand.open @14:04:56.41 Forearm.reconfigure LooseForearm @14:04:56.46 Forearm.seek :retract @14:04:57.45 SC.coast @14:04:57.50 collectSample 1500ml w/bubble point of 55psi @14:04:57.51 Priming 7ml @14:04:57.52 Intake.select :close @14:04:57.53 Exhaust.select :close @14:04:57.55 SS.hold @14:04:57.78 Calibrating pressure and temperature sensors @14:04:57.80 CTV.dial :air @14:04:57.82 SSV.dial :spit,avoiding: :exhaust @14:04:57.84 SS.reconfigure SSfast @14:04:57.89 SS.seek 5ml @14:05:00.60 SSV.dial CBV @14:05:00.63
CBV.dial SSV @14:05:02.02 delay 3.8 seconds @14:05:05.96 SSV.dial :spit @14:05:07.31 SS.seek 0ml @14:05:09.90 SSV.dial CBV @14:05:11.24 CTV.dial :intake @14:05:12.01 SSV.dial CBV @14:05:12.04
CBV.dial SSV @14:05:12.06 Allowing 40s for 7.0ml of remaining 7.0ml @14:05:12.07 Intake.select :open @14:05:14.25 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:05:14.26 SS.reconfigure SSinhale.with maxSpeed: 150 @14:05:14.31 SS.seek 7ml @14:05:17.40 Vacuum too high -- waiting up to 40s @14:05:19.00 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:05:19.02 SS.reconfigure SSinhale.with maxSpeed: 135 @14:05:19.07 SS.seek 7ml @14:05:21.78 Vacuum too high -- waiting up to 40s @14:05:23.40 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:05:23.41 SS.reconfigure SSinhale.with maxSpeed: 122 @14:05:23.46 SS.seek 7ml @14:05:25.57 Stroke completed -- waiting for pressure to equalize ... @14:05:28.17 Intake.select :close @14:05:28.33 SSV.dial :exhaust @14:05:29.11 Exhaust.select :open @14:05:29.22 SS.reconfigure SSexhale @14:05:29.27 SS.seek 0ml @14:05:33.04 Exhaust.select :close @14:05:33.16 Sampling 1500.0ml @14:05:33.17 Intake.select :close @14:05:33.18 Exhaust.select :close @14:05:33.20 SS.reconfigure SSconfig @14:05:33.24 SS.hold @14:05:33.46 CTV.dial :intake @14:05:33.48 SSV.dial CBV @14:05:33.54
CBV.dial SSV @14:05:34.30 Allowing 130s for 25.0ml of remaining 1500.0ml @14:05:34.31 Intake.select :open @14:05:36.49 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:05:36.50 SS.reconfigure SSinhale.with maxSpeed: 122 @14:05:36.55 SS.seek 25ml @14:05:39.61 Vacuum too high -- waiting up to 40s @14:05:40.78 delay 1 second @14:05:40.99
Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:05:41.01 SS.reconfigure SSinhale.with maxSpeed: 110 @14:05:41.06 SS.seek 25ml @14:05:41.81 AFV.select :holdingCoil @14:05:41.92 delay 1 second @14:05:42.95 ATV.dial :waste @14:05:43.78
Vacuum too high -- waiting up to 40s @14:05:43.93 AS.reconfigure ASnorm @14:05:43.98 AS.home.seek :empty @14:05:45.25
Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:05:45.27 SS.reconfigure SSinhale.with maxSpeed: 99 @14:05:45.32 SS.seek 25ml @14:05:47.80 Vacuum too high -- waiting up to 40s @14:05:49.12 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:05:49.14 SS.reconfigure SSinhale.with maxSpeed: 89 @14:05:49.19 SS.seek 25ml @14:05:51.70 Vacuum too high -- waiting up to 40s @14:05:53.56 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:05:53.58 SS.reconfigure SSinhale.with maxSpeed: 80 @14:05:53.63 SS.seek 25ml @14:05:56.96 Vacuum too high -- waiting up to 40s @14:05:58.07 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:05:58.08 SS.reconfigure SSinhale.with maxSpeed: 72 @14:05:58.13 SS.seek 25ml @14:06:00.95 Vacuum too high -- waiting up to 40s @14:06:02.07 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:06:02.09 SS.reconfigure SSinhale.with maxSpeed: 65 @14:06:02.14 SS.seek 25ml @14:06:06.00 Vacuum too high -- waiting up to 40s @14:06:07.11 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:06:07.13 SS.reconfigure SSinhale.with maxSpeed: 59 @14:06:07.18 SS.seek 25ml @14:06:11.64 Vacuum too high -- waiting up to 40s @14:06:12.75 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:06:12.77 SS.reconfigure SSinhale.with maxSpeed: 53 @14:06:12.82 SS.seek 25ml @14:06:17.93 Stroke completed -- waiting for pressure to equalize ... @14:06:20.53 Intake.select :close @14:06:20.69 SSV.dial :exhaust @14:06:21.47 Exhaust.select :open @14:06:21.58 SS.reconfigure SSexhale @14:06:21.63 SS.seek 0ml @14:06:30.56 Exhaust.select :close @14:06:30.69 SSV.dial CBV @14:06:30.72
CBV.dial SSV @14:06:31.52 Allowing 130s for 25.0ml of remaining 1475.0ml @14:06:31.53 Intake.select :open @14:06:33.70 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:06:33.72 SS.reconfigure SSinhale.with maxSpeed: 53 @14:06:33.77 SS.seek 25ml @14:06:36.59 ATV.dial :waste @14:06:36.61 ARV.dial between carrier and air1 @14:06:36.69 ASV.dial :waste1 @14:06:42.74
Vacuum too high -- waiting up to 40s @14:06:43.56 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:06:43.58 SS.reconfigure SSinhale.with maxSpeed: 48 @14:06:43.63 SS.seek 25ml @14:07:11.43 Stroke completed -- waiting for pressure to equalize ... @14:07:14.00 Intake.select :close @14:07:14.15 SSV.dial :exhaust @14:07:14.93 Exhaust.select :open @14:07:15.05 SS.reconfigure SSexhale @14:07:15.10 SS.seek 0ml @14:07:24.15 Exhaust.select :close @14:07:24.79 SSV.dial CBV @14:07:24.82
CBV.dial SSV @14:07:25.61 Allowing 130s for 25.0ml of remaining 1450.0ml @14:07:25.62 Intake.select :open @14:07:27.80 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:07:27.81 SS.reconfigure SSinhale.with maxSpeed: 48 @14:07:27.86 SS.seek 25ml @14:08:05.14 Stroke completed -- waiting for pressure to equalize ... @14:08:07.74 Intake.select :close @14:08:07.89 SSV.dial :exhaust @14:08:08.67 Exhaust.select :open @14:08:08.79 SS.reconfigure SSexhale @14:08:08.84 SS.seek 0ml @14:08:17.95 Exhaust.select :close @14:08:18.07 SSV.dial CBV @14:08:18.10
CBV.dial SSV @14:08:18.90 Allowing 130s for 25.0ml of remaining 1425.0ml @14:08:18.91 Intake.select :open @14:08:21.09 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:08:21.10 SS.reconfigure SSinhale.with maxSpeed: 48 @14:08:21.15 SS.seek 25ml @14:08:51.70 Vacuum too high -- waiting up to 40s @14:08:52.53 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:08:52.55 SS.reconfigure SSinhale.with maxSpeed: 43 @14:08:52.60 SS.seek 25ml @14:08:59.85 Stroke completed -- waiting for pressure to equalize ... @14:09:02.45 Intake.select :close @14:09:02.61 SSV.dial :exhaust @14:09:03.38 Exhaust.select :open @14:09:03.49 SS.reconfigure SSexhale @14:09:03.54 SS.seek 0ml @14:09:12.39 Exhaust.select :close @14:09:12.51 SSV.dial CBV @14:09:12.54
CBV.dial SSV @14:09:13.35 Allowing 130s for 25.0ml of remaining 1400.0ml @14:09:13.36 Intake.select :open @14:09:15.53 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:09:15.55 SS.reconfigure SSinhale.with maxSpeed: 43 @14:09:15.60 SS.seek 25ml @14:09:57.24 Stroke completed -- waiting for pressure to equalize ... @14:09:59.81 Intake.select :close @14:09:59.97 SSV.dial :exhaust @14:10:00.73 Exhaust.select :open @14:10:00.85 SS.reconfigure SSexhale @14:10:00.90 SS.seek 0ml @14:10:10.14 Exhaust.select :close @14:10:10.74 SSV.dial CBV @14:10:11.07
CBV.dial SSV @14:10:12.42 Allowing 130s for 25.0ml of remaining 1375.0ml @14:10:12.53 Intake.select :open @14:10:16.04 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:10:16.25 SS.reconfigure SSinhale.with maxSpeed: 43 @14:10:16.52 SS.seek 25ml @14:10:57.84 Stroke completed -- waiting for pressure to equalize ... @14:11:00.46 Intake.select :close @14:11:00.62 SSV.dial :exhaust @14:11:01.40 Exhaust.select :open @14:11:01.51 SS.reconfigure SSexhale @14:11:01.56 SS.seek 0ml @14:11:10.71 Exhaust.select :close @14:11:11.34 SSV.dial CBV @14:11:11.37
CBV.dial SSV @14:11:12.18 Allowing 130s for 25.0ml of remaining 1350.0ml @14:11:12.19 Intake.select :open @14:11:14.38 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:11:14.39 SS.reconfigure SSinhale.with maxSpeed: 43 @14:11:14.44 SS.seek 25ml @14:11:55.43 Stroke completed -- waiting for pressure to equalize ... @14:11:58.06 Intake.select :close @14:11:58.22 SSV.dial :exhaust @14:11:58.99 Exhaust.select :open @14:11:59.10 SS.reconfigure SSexhale @14:11:59.15 SS.seek 0ml @14:12:08.31 Exhaust.select :close @14:12:08.43 SSV.dial CBV @14:12:08.46
CBV.dial SSV @14:12:09.27 Allowing 130s for 25.0ml of remaining 1325.0ml @14:12:09.28 Intake.select :open @14:12:11.47 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:12:11.48 SS.reconfigure SSinhale.with maxSpeed: 43 @14:12:11.53 SS.seek 25ml @14:12:52.48 Stroke completed -- waiting for pressure to equalize ... @14:12:55.09 Intake.select :close @14:12:55.26 SSV.dial :exhaust @14:12:56.04 Exhaust.select :open @14:12:56.15 SS.reconfigure SSexhale @14:12:56.20 SS.seek 0ml @14:13:05.35 Exhaust.select :close @14:13:05.47 SSV.dial CBV @14:13:05.50
CBV.dial SSV @14:13:06.30 Allowing 130s for 25.0ml of remaining 1300.0ml @14:13:06.31 Intake.select :open @14:13:08.49 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:13:08.50 SS.reconfigure SSinhale.with maxSpeed: 43 @14:13:08.55 SS.seek 25ml @14:13:49.42 Stroke completed -- waiting for pressure to equalize ... @14:13:52.03 Intake.select :close @14:13:52.19 SSV.dial :exhaust @14:13:52.96 Exhaust.select :open @14:13:53.07 SS.reconfigure SSexhale @14:13:53.12 SS.seek 0ml @14:14:02.14 Exhaust.select :close @14:14:02.26 SSV.dial CBV @14:14:02.29
CBV.dial SSV @14:14:03.10 Allowing 130s for 25.0ml of remaining 1275.0ml @14:14:03.11 Intake.select :open @14:14:05.28 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:14:05.30 SS.reconfigure SSinhale.with maxSpeed: 43 @14:14:05.35 SS.seek 25ml @14:14:46.67 Stroke completed -- waiting for pressure to equalize ... @14:14:49.28 Intake.select :close @14:14:49.44 SSV.dial :exhaust @14:14:50.21 Exhaust.select :open @14:14:50.32 SS.reconfigure SSexhale @14:14:50.37 SS.seek 0ml @14:14:59.45 Exhaust.select :close @14:14:59.57 SSV.dial CBV @14:14:59.60
CBV.dial SSV @14:15:00.41 Allowing 130s for 25.0ml of remaining 1250.0ml @14:15:00.42 Intake.select :open @14:15:02.59 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:15:02.61 SS.reconfigure SSinhale.with maxSpeed: 43 @14:15:02.67 SS.seek 25ml @14:15:43.95 Stroke completed -- waiting for pressure to equalize ... @14:15:46.43 Intake.select :close @14:15:46.59 SSV.dial :exhaust @14:15:47.38 Exhaust.select :open @14:15:47.50 SS.reconfigure SSexhale @14:15:47.55 SS.seek 0ml @14:15:56.76 Exhaust.select :close @14:15:56.88 SSV.dial CBV @14:15:56.91
CBV.dial SSV @14:15:57.72 Allowing 130s for 25.0ml of remaining 1225.0ml @14:15:57.73 Intake.select :open @14:15:59.91 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:15:59.93 SS.reconfigure SSinhale.with maxSpeed: 43 @14:15:59.99 SS.seek 25ml @14:16:41.39 Stroke completed -- waiting for pressure to equalize ... @14:16:44.02 Intake.select :close @14:16:44.18 SSV.dial :exhaust @14:16:44.96 Exhaust.select :open @14:16:45.07 SS.reconfigure SSexhale @14:16:45.12 SS.seek 0ml @14:16:54.06 Exhaust.select :close @14:16:54.18 SSV.dial CBV @14:16:54.21
CBV.dial SSV @14:16:55.02 Allowing 130s for 25.0ml of remaining 1200.0ml @14:16:55.03 Intake.select :open @14:16:57.21 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:16:57.22 SS.reconfigure SSinhale.with maxSpeed: 43 @14:16:57.27 SS.seek 25ml @14:17:38.53 Stroke completed -- waiting for pressure to equalize ... @14:17:41.13 Intake.select :close @14:17:41.29 SSV.dial :exhaust @14:17:42.07 Exhaust.select :open @14:17:42.18 SS.reconfigure SSexhale @14:17:42.23 SS.seek 0ml @14:17:50.98 Exhaust.select :close @14:17:51.10 SSV.dial CBV @14:17:51.13
CBV.dial SSV @14:17:51.93 Allowing 130s for 25.0ml of remaining 1175.0ml @14:17:51.94 Intake.select :open @14:17:54.11 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:17:54.13 SS.reconfigure SSinhale.with maxSpeed: 43 @14:17:54.18 SS.seek 25ml @14:18:35.95 Stroke completed -- waiting for pressure to equalize ... @14:18:38.54 Intake.select :close @14:18:38.70 SSV.dial :exhaust @14:18:39.47 Exhaust.select :open @14:18:39.58 SS.reconfigure SSexhale @14:18:39.63 SS.seek 0ml @14:18:48.60 Exhaust.select :close @14:18:48.72 SSV.dial CBV @14:18:48.75
CBV.dial SSV @14:18:49.55 Allowing 130s for 25.0ml of remaining 1150.0ml @14:18:49.56 Intake.select :open @14:18:51.73 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:18:51.75 SS.reconfigure SSinhale.with maxSpeed: 43 @14:18:51.80 SS.seek 25ml @14:19:33.17 Stroke completed -- waiting for pressure to equalize ... @14:19:35.78 Intake.select :close @14:19:35.94 SSV.dial :exhaust @14:19:36.71 Exhaust.select :open @14:19:36.82 SS.reconfigure SSexhale @14:19:36.87 SS.seek 0ml @14:19:45.62 Exhaust.select :close @14:19:46.25 SSV.dial CBV @14:19:46.28
CBV.dial SSV @14:19:47.10 Allowing 130s for 25.0ml of remaining 1125.0ml @14:19:47.11 Intake.select :open @14:19:49.29 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:19:49.31 SS.reconfigure SSinhale.with maxSpeed: 43 @14:19:49.36 SS.seek 25ml @14:20:31.01 Stroke completed -- waiting for pressure to equalize ... @14:20:33.63 Intake.select :close @14:20:33.79 SSV.dial :exhaust @14:20:34.57 Exhaust.select :open @14:20:34.68 SS.reconfigure SSexhale @14:20:34.73 SS.seek 0ml @14:20:44.14 Exhaust.select :close @14:20:44.26 SSV.dial CBV @14:20:44.29
CBV.dial SSV @14:20:45.08 Allowing 130s for 25.0ml of remaining 1100.0ml @14:20:45.09 Intake.select :open @14:20:47.28 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:20:47.29 SS.reconfigure SSinhale.with maxSpeed: 43 @14:20:47.35 SS.seek 25ml @14:21:28.26 Stroke completed -- waiting for pressure to equalize ... @14:21:30.88 Intake.select :close @14:21:31.04 SSV.dial :exhaust @14:21:31.83 Exhaust.select :open @14:21:31.94 SS.reconfigure SSexhale @14:21:31.99 SS.seek 0ml @14:21:41.04 Exhaust.select :close @14:21:41.16 SSV.dial CBV @14:21:41.19
CBV.dial SSV @14:21:42.00 Allowing 130s for 25.0ml of remaining 1075.0ml @14:21:42.01 Intake.select :open @14:21:44.20 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:21:44.21 SS.reconfigure SSinhale.with maxSpeed: 43 @14:21:44.26 SS.seek 25ml @14:22:25.51 Stroke completed -- waiting for pressure to equalize ... @14:22:28.11 Intake.select :close @14:22:28.27 SSV.dial :exhaust @14:22:29.05 Exhaust.select :open @14:22:29.16 SS.reconfigure SSexhale @14:22:29.21 SS.seek 0ml @14:22:38.42 Exhaust.select :close @14:22:38.54 SSV.dial CBV @14:22:38.57
CBV.dial SSV @14:22:39.36 Allowing 130s for 25.0ml of remaining 1050.0ml @14:22:39.37 Intake.select :open @14:22:41.55 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:22:41.56 SS.reconfigure SSinhale.with maxSpeed: 43 @14:22:41.61 SS.seek 25ml @14:23:23.07 Stroke completed -- waiting for pressure to equalize ... @14:23:25.67 Intake.select :close @14:23:25.83 SSV.dial :exhaust @14:23:26.61 Exhaust.select :open @14:23:26.72 SS.reconfigure SSexhale @14:23:26.77 SS.seek 0ml @14:23:35.82 Exhaust.select :close @14:23:35.94 SSV.dial CBV @14:23:35.97
CBV.dial SSV @14:23:36.77 Allowing 130s for 25.0ml of remaining 1025.0ml @14:23:36.78 Intake.select :open @14:23:38.95 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:23:38.97 SS.reconfigure SSinhale.with maxSpeed: 43 @14:23:39.02 SS.seek 25ml @14:24:20.21 Stroke completed -- waiting for pressure to equalize ... @14:24:22.89 Intake.select :close @14:24:23.05 SSV.dial :exhaust @14:24:23.83 Exhaust.select :open @14:24:23.95 SS.reconfigure SSexhale @14:24:23.99 SS.seek 0ml @14:24:32.98 Exhaust.select :close @14:24:33.10 SSV.dial CBV @14:24:33.13
CBV.dial SSV @14:24:33.94 Allowing 130s for 25.0ml of remaining 1000.0ml @14:24:33.95 Intake.select :open @14:24:36.14 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:24:36.15 SS.reconfigure SSinhale.with maxSpeed: 43 @14:24:36.21 SS.seek 25ml @14:25:17.43 Stroke completed -- waiting for pressure to equalize ... @14:25:20.06 Intake.select :close @14:25:20.22 SSV.dial :exhaust @14:25:20.99 Exhaust.select :open @14:25:21.11 SS.reconfigure SSexhale @14:25:21.16 SS.seek 0ml @14:25:29.92 Exhaust.select :close @14:25:30.04 SSV.dial CBV @14:25:30.07
CBV.dial SSV @14:25:30.88 Allowing 130s for 25.0ml of remaining 975.0ml @14:25:30.89 Intake.select :open @14:25:33.07 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:25:33.08 SS.reconfigure SSinhale.with maxSpeed: 43 @14:25:33.13 SS.seek 25ml @14:26:14.48 Stroke completed -- waiting for pressure to equalize ... @14:26:17.08 Intake.select :close @14:26:17.24 SSV.dial :exhaust @14:26:18.02 Exhaust.select :open @14:26:18.13 SS.reconfigure SSexhale @14:26:18.18 SS.seek 0ml @14:26:26.98 Exhaust.select :close @14:26:27.10 SSV.dial CBV @14:26:27.13
CBV.dial SSV @14:26:27.94 Allowing 130s for 25.0ml of remaining 950.0ml @14:26:27.95 Intake.select :open @14:26:30.14 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:26:30.15 SS.reconfigure SSinhale.with maxSpeed: 43 @14:26:30.20 SS.seek 25ml @14:27:11.87 Stroke completed -- waiting for pressure to equalize ... @14:27:14.49 Intake.select :close @14:27:14.65 SSV.dial :exhaust @14:27:15.43 Exhaust.select :open @14:27:15.54 SS.reconfigure SSexhale @14:27:15.59 SS.seek 0ml @14:27:24.65 Exhaust.select :close @14:27:24.77 SSV.dial CBV @14:27:24.80
CBV.dial SSV @14:27:25.60 Allowing 130s for 25.0ml of remaining 925.0ml @14:27:25.61 Intake.select :open @14:27:27.79 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:27:27.80 SS.reconfigure SSinhale.with maxSpeed: 43 @14:27:27.85 SS.seek 25ml @14:28:09.26 Stroke completed -- waiting for pressure to equalize ... @14:28:12.50 Intake.select :close @14:28:12.66 SSV.dial :exhaust @14:28:13.94 Exhaust.select :open @14:28:14.05 SS.reconfigure SSexhale @14:28:14.10 SS.seek 0ml @14:28:22.85 Exhaust.select :close @14:28:22.97 SSV.dial CBV @14:28:23.00
CBV.dial SSV @14:28:23.80 Allowing 130s for 25.0ml of remaining 900.0ml @14:28:23.81 Intake.select :open @14:28:25.99 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:28:26.01 SS.reconfigure SSinhale.with maxSpeed: 43 @14:28:26.06 SS.seek 25ml @14:29:06.84 Vacuum too high -- waiting up to 40s @14:29:07.68 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:29:07.69 SS.reconfigure SSinhale.with maxSpeed: 39 @14:29:07.75 SS.seek 25ml @14:29:08.88 Stroke completed -- waiting for pressure to equalize ... @14:29:10.86 Intake.select :close @14:29:11.02 SSV.dial :exhaust @14:29:11.80 Exhaust.select :open @14:29:11.92 SS.reconfigure SSexhale @14:29:11.97 SS.seek 0ml @14:29:21.09 Exhaust.select :close @14:29:21.21 SSV.dial CBV @14:29:21.24
CBV.dial SSV @14:29:22.04 Allowing 130s for 25.0ml of remaining 875.0ml @14:29:22.05 Intake.select :open @14:29:24.23 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:29:24.24 SS.reconfigure SSinhale.with maxSpeed: 39 @14:29:24.30 SS.seek 25ml @14:30:09.35 Stroke completed -- waiting for pressure to equalize ... @14:30:11.96 Intake.select :close @14:30:12.12 SSV.dial :exhaust @14:30:12.91 Exhaust.select :open @14:30:13.02 SS.reconfigure SSexhale @14:30:13.07 SS.seek 0ml @14:30:22.24 Exhaust.select :close @14:30:22.36 SSV.dial CBV @14:30:22.39
CBV.dial SSV @14:30:23.19 Allowing 130s for 25.0ml of remaining 850.0ml @14:30:23.20 Intake.select :open @14:30:25.38 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:30:25.39 SS.reconfigure SSinhale.with maxSpeed: 39 @14:30:25.44 SS.seek 25ml @14:31:10.90 Stroke completed -- waiting for pressure to equalize ... @14:31:13.52 Intake.select :close @14:31:13.68 SSV.dial :exhaust @14:31:14.46 Exhaust.select :open @14:31:14.57 SS.reconfigure SSexhale @14:31:14.62 SS.seek 0ml @14:31:23.71 Exhaust.select :close @14:31:23.83 SSV.dial CBV @14:31:23.86
CBV.dial SSV @14:31:24.66 Allowing 130s for 25.0ml of remaining 825.0ml @14:31:24.67 Intake.select :open @14:31:26.84 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:31:26.86 SS.reconfigure SSinhale.with maxSpeed: 39 @14:31:26.91 SS.seek 25ml @14:32:12.29 Stroke completed -- waiting for pressure to equalize ... @14:32:14.88 Intake.select :close @14:32:15.04 SSV.dial :exhaust @14:32:15.83 Exhaust.select :open @14:32:15.94 SS.reconfigure SSexhale @14:32:16.50 SS.seek 0ml @14:32:25.59 Exhaust.select :close @14:32:25.71 SSV.dial CBV @14:32:25.74
CBV.dial SSV @14:32:26.57 Allowing 130s for 25.0ml of remaining 800.0ml @14:32:26.58 Intake.select :open @14:32:28.77 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:32:28.79 SS.reconfigure SSinhale.with maxSpeed: 39 @14:32:28.84 SS.seek 25ml @14:33:13.96 Stroke completed -- waiting for pressure to equalize ... @14:33:16.57 Intake.select :close @14:33:16.73 SSV.dial :exhaust @14:33:17.50 Exhaust.select :open @14:33:17.61 SS.reconfigure SSexhale @14:33:17.66 SS.seek 0ml @14:33:26.67 Exhaust.select :close @14:33:26.79 SSV.dial CBV @14:33:26.82
CBV.dial SSV @14:33:27.61 Allowing 130s for 25.0ml of remaining 775.0ml @14:33:27.62 Intake.select :open @14:33:29.81 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:33:29.82 SS.reconfigure SSinhale.with maxSpeed: 39 @14:33:29.88 SS.seek 25ml @14:34:15.26 Stroke completed -- waiting for pressure to equalize ... @14:34:17.87 Intake.select :close @14:34:18.03 SSV.dial :exhaust @14:34:18.80 Exhaust.select :open @14:34:18.91 SS.reconfigure SSexhale @14:34:18.96 SS.seek 0ml @14:34:28.13 Exhaust.select :close @14:34:28.25 SSV.dial CBV @14:34:28.28
CBV.dial SSV @14:34:29.08 Allowing 130s for 25.0ml of remaining 750.0ml @14:34:29.09 Intake.select :open @14:34:31.27 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:34:31.28 SS.reconfigure SSinhale.with maxSpeed: 39 @14:34:31.34 SS.seek 25ml @14:35:16.71 Stroke completed -- waiting for pressure to equalize ... @14:35:19.94 Intake.select :close @14:35:20.10 SSV.dial :exhaust @14:35:20.88 Exhaust.select :open @14:35:20.99 SS.reconfigure SSexhale @14:35:21.04 SS.seek 0ml @14:35:29.71 Exhaust.select :close @14:35:29.83 SSV.dial CBV @14:35:29.86
CBV.dial SSV @14:35:30.66 Allowing 130s for 25.0ml of remaining 725.0ml @14:35:30.67 Intake.select :open @14:35:32.85 Intake @34.4psia, vacuum from 20.0 to 24.0psi @14:35:32.86 SS.reconfigure SSinhale.with maxSpeed: 39 @14:35:32.91 SS.seek 25ml @14:36:18.65 Stroke completed -- waiting for pressure to equalize ... @14:36:21.88 Intake.select :close @14:36:22.04 SSV.dial :exhaust @14:36:22.82 Exhaust.select :open @14:36:22.93 SS.reconfigure SSexhale @14:36:22.98 SS.seek 0ml @14:36:32.10 Exhaust.select :close @14:36:32.22 SSV.dial CBV @14:36:32.25
CBV.dial SSV @14:36:33.05 Allowing 130s for 25.0ml of remaining 700.0ml @14:36:33.06 Intake.select :open @14:36:35.75 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:36:35.77 SS.reconfigure SSinhale.with maxSpeed: 39 @14:36:35.82 SS.seek 25ml @14:37:21.23 Stroke completed -- waiting for pressure to equalize ... @14:37:24.49 Intake.select :close @14:37:24.65 SSV.dial :exhaust @14:37:25.43 Exhaust.select :open @14:37:25.54 SS.reconfigure SSexhale @14:37:25.59 SS.seek 0ml @14:37:34.32 Exhaust.select :close @14:37:34.44 SSV.dial CBV @14:37:34.47
CBV.dial SSV @14:37:35.27 Allowing 130s for 25.0ml of remaining 675.0ml @14:37:35.28 Intake.select :open @14:37:37.45 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:37:37.47 SS.reconfigure SSinhale.with maxSpeed: 39 @14:37:37.52 SS.seek 25ml @14:38:23.29 Stroke completed -- waiting for pressure to equalize ... @14:38:26.54 Intake.select :close @14:38:26.70 SSV.dial :exhaust @14:38:27.47 Exhaust.select :open @14:38:27.58 SS.reconfigure SSexhale @14:38:27.63 SS.seek 0ml @14:38:36.71 Exhaust.select :close @14:38:36.83 SSV.dial CBV @14:38:36.86
CBV.dial SSV @14:38:37.66 Allowing 130s for 25.0ml of remaining 650.0ml @14:38:37.67 Intake.select :open @14:38:39.86 Intake @34.4psia, vacuum from 20.0 to 24.0psi @14:38:39.87 SS.reconfigure SSinhale.with maxSpeed: 39 @14:38:39.93 SS.seek 25ml @14:39:25.15 Stroke completed -- waiting for pressure to equalize ... @14:39:28.37 Intake.select :close @14:39:28.53 SSV.dial :exhaust @14:39:29.30 Exhaust.select :open @14:39:29.41 SS.reconfigure SSexhale @14:39:29.46 SS.seek 0ml @14:39:38.23 Exhaust.select :close @14:39:38.35 SSV.dial CBV @14:39:38.38
CBV.dial SSV @14:39:39.19 Allowing 130s for 25.0ml of remaining 625.0ml @14:39:39.20 Intake.select :open @14:39:41.38 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:39:41.39 SS.reconfigure SSinhale.with maxSpeed: 39 @14:39:41.44 SS.seek 25ml @14:40:23.98 Vacuum too high -- waiting up to 40s @14:40:24.83 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:40:24.85 SS.reconfigure SSinhale.with maxSpeed: 35 @14:40:24.90 SS.seek 25ml @14:40:28.21 Stroke completed -- waiting for pressure to equalize ... @14:40:30.82 Intake.select :close @14:40:30.98 SSV.dial :exhaust @14:40:31.75 Exhaust.select :open @14:40:31.86 SS.reconfigure SSexhale @14:40:31.91 SS.seek 0ml @14:40:40.88 Exhaust.select :close @14:40:41.52 SSV.dial CBV @14:40:41.55
CBV.dial SSV @14:40:42.35 Allowing 130s for 25.0ml of remaining 600.0ml @14:40:42.36 Intake.select :open @14:40:44.54 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:40:44.56 SS.reconfigure SSinhale.with maxSpeed: 35 @14:40:44.61 SS.seek 25ml @14:41:35.04 Stroke completed -- waiting for pressure to equalize ... @14:41:38.27 Intake.select :close @14:41:38.43 SSV.dial :exhaust @14:41:39.21 Exhaust.select :open @14:41:39.32 SS.reconfigure SSexhale @14:41:39.37 SS.seek 0ml @14:41:48.38 Exhaust.select :close @14:41:48.50 SSV.dial CBV @14:41:48.53
CBV.dial SSV @14:41:49.32 Allowing 130s for 25.0ml of remaining 575.0ml @14:41:49.33 Intake.select :open @14:41:51.51 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:41:51.53 SS.reconfigure SSinhale.with maxSpeed: 35 @14:41:51.58 SS.seek 25ml @14:42:42.01 Stroke completed -- waiting for pressure to equalize ... @14:42:45.26 Intake.select :close @14:42:45.42 SSV.dial :exhaust @14:42:46.19 Exhaust.select :open @14:42:46.30 SS.reconfigure SSexhale @14:42:46.35 SS.seek 0ml @14:42:55.48 Exhaust.select :close @14:42:55.60 SSV.dial CBV @14:42:55.63
CBV.dial SSV @14:42:56.44 Allowing 130s for 25.0ml of remaining 550.0ml @14:42:56.45 Intake.select :open @14:42:58.64 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:42:58.65 SS.reconfigure SSinhale.with maxSpeed: 35 @14:42:58.71 SS.seek 25ml @14:43:48.88 Stroke completed -- waiting for pressure to equalize ... @14:43:52.10 Intake.select :close @14:43:52.26 SSV.dial :exhaust @14:43:53.03 Exhaust.select :open @14:43:53.14 SS.reconfigure SSexhale @14:43:53.19 SS.seek 0ml @14:44:02.20 Exhaust.select :close @14:44:02.32 SSV.dial CBV @14:44:02.35
CBV.dial SSV @14:44:03.16 Allowing 130s for 25.0ml of remaining 525.0ml @14:44:03.17 Intake.select :open @14:44:05.36 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:44:05.37 SS.reconfigure SSinhale.with maxSpeed: 35 @14:44:05.42 SS.seek 25ml @14:44:55.59 Stroke completed -- waiting for pressure to equalize ... @14:44:58.82 Intake.select :close @14:44:58.98 SSV.dial :exhaust @14:44:59.75 Exhaust.select :open @14:44:59.86 SS.reconfigure SSexhale @14:44:59.91 SS.seek 0ml @14:45:08.70 Exhaust.select :close @14:45:08.82 SSV.dial CBV @14:45:08.85
CBV.dial SSV @14:45:09.66 Allowing 130s for 25.0ml of remaining 500.0ml @14:45:10.14 Intake.select :open @14:45:12.36 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:45:12.38 SS.reconfigure SSinhale.with maxSpeed: 35 @14:45:12.43 SS.seek 25ml @14:46:03.17 Stroke completed -- waiting for pressure to equalize ... @14:46:06.41 Intake.select :close @14:46:06.57 SSV.dial :exhaust @14:46:07.35 Exhaust.select :open @14:46:07.46 SS.reconfigure SSexhale @14:46:07.51 SS.seek 0ml @14:46:16.67 Exhaust.select :close @14:46:16.79 SSV.dial CBV @14:46:16.82
CBV.dial SSV @14:46:17.63 Allowing 130s for 25.0ml of remaining 475.0ml @14:46:17.64 Intake.select :open @14:46:19.82 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:46:19.84 SS.reconfigure SSinhale.with maxSpeed: 35 @14:46:19.89 SS.seek 25ml @14:47:10.13 Stroke completed -- waiting for pressure to equalize ... @14:47:13.36 Intake.select :close @14:47:13.52 SSV.dial :exhaust @14:47:14.30 Exhaust.select :open @14:47:14.41 SS.reconfigure SSexhale @14:47:14.46 SS.seek 0ml @14:47:23.31 Exhaust.select :close @14:47:23.43 SSV.dial CBV @14:47:23.46
CBV.dial SSV @14:47:24.27 Allowing 130s for 25.0ml of remaining 450.0ml @14:47:24.28 Intake.select :open @14:47:26.45 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:47:26.47 SS.reconfigure SSinhale.with maxSpeed: 35 @14:47:26.52 SS.seek 25ml @14:48:16.99 Stroke completed -- waiting for pressure to equalize ... @14:48:20.22 Intake.select :close @14:48:20.38 SSV.dial :exhaust @14:48:21.18 Exhaust.select :open @14:48:21.29 SS.reconfigure SSexhale @14:48:21.34 SS.seek 0ml @14:48:30.12 Exhaust.select :close @14:48:30.24 SSV.dial CBV @14:48:30.27
CBV.dial SSV @14:48:31.08 Allowing 130s for 25.0ml of remaining 425.0ml @14:48:31.09 Intake.select :open @14:48:33.27 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:48:33.29 SS.reconfigure SSinhale.with maxSpeed: 35 @14:48:33.34 SS.seek 25ml @14:49:24.10 Stroke completed -- waiting for pressure to equalize ... @14:49:27.97 Intake.select :close @14:49:28.13 SSV.dial :exhaust @14:49:28.93 Exhaust.select :open @14:49:29.04 SS.reconfigure SSexhale @14:49:29.09 SS.seek 0ml @14:49:38.12 Exhaust.select :close @14:49:38.24 SSV.dial CBV @14:49:38.27
CBV.dial SSV @14:49:39.10 Allowing 130s for 25.0ml of remaining 400.0ml @14:49:39.11 Intake.select :open @14:49:41.79 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:49:41.81 SS.reconfigure SSinhale.with maxSpeed: 35 @14:49:41.86 SS.seek 25ml @14:50:32.17 Stroke completed -- waiting for pressure to equalize ... @14:50:36.05 Intake.select :close @14:50:36.21 SSV.dial :exhaust @14:50:37.00 Exhaust.select :open @14:50:37.11 SS.reconfigure SSexhale @14:50:37.16 SS.seek 0ml @14:50:46.45 Exhaust.select :close @14:50:46.57 SSV.dial CBV @14:50:46.60
CBV.dial SSV @14:50:47.43 Allowing 130s for 25.0ml of remaining 375.0ml @14:50:47.44 Intake.select :open @14:50:49.63 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:50:49.64 SS.reconfigure SSinhale.with maxSpeed: 35 @14:50:49.70 SS.seek 25ml @14:51:28.71 Vacuum too high -- waiting up to 40s @14:51:29.57 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:51:29.58 SS.reconfigure SSinhale.with maxSpeed: 32 @14:51:29.64 SS.seek 25ml @14:51:42.34 Stroke completed -- waiting for pressure to equalize ... @14:51:45.59 Intake.select :close @14:51:45.75 SSV.dial :exhaust @14:51:46.52 Exhaust.select :open @14:51:46.63 SS.reconfigure SSexhale @14:51:46.68 SS.seek 0ml @14:51:55.82 Exhaust.select :close @14:51:55.94 SSV.dial CBV @14:51:55.97
CBV.dial SSV @14:51:56.75 Allowing 130s for 25.0ml of remaining 350.0ml @14:51:56.76 Intake.select :open @14:51:58.94 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:51:58.95 SS.reconfigure SSinhale.with maxSpeed: 32 @14:51:59.00 SS.seek 25ml @14:52:53.85 Stroke completed -- waiting for pressure to equalize ... @14:52:57.09 Intake.select :close @14:52:57.25 SSV.dial :exhaust @14:52:58.02 Exhaust.select :open @14:52:58.13 SS.reconfigure SSexhale @14:52:58.18 SS.seek 0ml @14:53:07.07 Exhaust.select :close @14:53:07.19 SSV.dial CBV @14:53:07.22
CBV.dial SSV @14:53:08.02 Allowing 130s for 25.0ml of remaining 325.0ml @14:53:08.03 Intake.select :open @14:53:10.21 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:53:10.22 SS.reconfigure SSinhale.with maxSpeed: 32 @14:53:10.27 SS.seek 25ml @14:54:05.60 Stroke completed -- waiting for pressure to equalize ... @14:54:09.47 Intake.select :close @14:54:09.62 SSV.dial :exhaust @14:54:10.92 Exhaust.select :open @14:54:11.03 SS.reconfigure SSexhale @14:54:11.08 SS.seek 0ml @14:54:19.92 Exhaust.select :close @14:54:20.04 SSV.dial CBV @14:54:20.07
CBV.dial SSV @14:54:20.88 Allowing 130s for 25.0ml of remaining 300.0ml @14:54:20.89 Intake.select :open @14:54:23.08 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:54:23.10 SS.reconfigure SSinhale.with maxSpeed: 32 @14:54:23.15 SS.seek 25ml @14:55:18.48 Stroke completed -- waiting for pressure to equalize ... @14:55:22.35 Intake.select :close @14:55:22.51 SSV.dial :exhaust @14:55:23.28 Exhaust.select :open @14:55:23.39 SS.reconfigure SSexhale @14:55:23.44 SS.seek 0ml @14:55:32.29 Exhaust.select :close @14:55:32.41 SSV.dial CBV @14:55:32.44
CBV.dial SSV @14:55:33.25 Allowing 130s for 25.0ml of remaining 275.0ml @14:55:33.26 Intake.select :open @14:55:35.44 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:55:35.45 SS.reconfigure SSinhale.with maxSpeed: 32 @14:55:35.51 SS.seek 25ml @14:56:30.85 Stroke completed -- waiting for pressure to equalize ... @14:56:34.72 Intake.select :close @14:56:34.88 SSV.dial :exhaust @14:56:35.66 Exhaust.select :open @14:56:35.77 SS.reconfigure SSexhale @14:56:35.82 SS.seek 0ml @14:56:44.87 Exhaust.select :close @14:56:44.99 SSV.dial CBV @14:56:45.02
CBV.dial SSV @14:56:45.83 Allowing 130s for 25.0ml of remaining 250.0ml @14:56:45.84 Intake.select :open @14:56:48.02 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:56:48.03 SS.reconfigure SSinhale.with maxSpeed: 32 @14:56:48.08 SS.seek 25ml @14:57:42.99 Stroke completed -- waiting for pressure to equalize ... @14:57:46.86 Intake.select :close @14:57:47.02 SSV.dial :exhaust @14:57:47.82 Exhaust.select :open @14:57:47.93 SS.reconfigure SSexhale @14:57:47.98 SS.seek 0ml @14:57:57.24 Exhaust.select :close @14:57:57.36 SSV.dial CBV @14:57:57.39
CBV.dial SSV @14:57:58.21 Allowing 130s for 25.0ml of remaining 225.0ml @14:57:58.22 Intake.select :open @14:58:00.39 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:58:00.41 SS.reconfigure SSinhale.with maxSpeed: 32 @14:58:00.46 SS.seek 25ml @14:58:55.01 Stroke completed -- waiting for pressure to equalize ... @14:58:59.38 Intake.select :close @14:58:59.54 SSV.dial :exhaust @14:59:00.34 Exhaust.select :open @14:59:00.45 SS.reconfigure SSexhale @14:59:00.50 SS.seek 0ml @14:59:09.49 Exhaust.select :close @14:59:09.61 SSV.dial CBV @14:59:09.64
CBV.dial SSV @14:59:10.47 Allowing 130s for 25.0ml of remaining 200.0ml @14:59:10.48 Intake.select :open @14:59:12.66 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:59:12.68 SS.reconfigure SSinhale.with maxSpeed: 32 @14:59:12.73 SS.seek 25ml @14:59:42.83 Vacuum too high -- waiting up to 40s @14:59:43.68 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:59:43.70 SS.reconfigure SSinhale.with maxSpeed: 29 @14:59:43.75 SS.seek 25ml @15:00:11.68 Stroke completed -- waiting for pressure to equalize ... @15:00:15.55 Intake.select :close @15:00:15.71 SSV.dial :exhaust @15:00:16.49 Exhaust.select :open @15:00:16.60 SS.reconfigure SSexhale @15:00:16.65 SS.seek 0ml @15:00:25.88 Exhaust.select :close @15:00:25.99 SSV.dial CBV @15:00:26.02
CBV.dial SSV @15:00:26.83 Allowing 130s for 25.0ml of remaining 175.0ml @15:00:26.84 Intake.select :open @15:00:29.01 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:00:29.03 SS.reconfigure SSinhale.with maxSpeed: 29 @15:00:29.08 SS.seek 25ml @15:01:29.66 Stroke completed -- waiting for pressure to equalize ... @15:01:33.54 Intake.select :close @15:01:33.70 SSV.dial :exhaust @15:01:34.49 Exhaust.select :open @15:01:34.60 SS.reconfigure SSexhale @15:01:34.65 SS.seek 0ml @15:01:43.70 Exhaust.select :close @15:01:43.82 SSV.dial CBV @15:01:43.85
CBV.dial SSV @15:01:44.66 Allowing 130s for 25.0ml of remaining 150.0ml @15:01:44.67 Intake.select :open @15:01:46.84 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:01:46.86 SS.reconfigure SSinhale.with maxSpeed: 29 @15:01:46.91 SS.seek 25ml @15:02:47.32 Stroke completed -- waiting for pressure to equalize ... @15:02:51.20 Intake.select :close @15:02:51.36 SSV.dial :exhaust @15:02:52.13 Exhaust.select :open @15:02:52.24 SS.reconfigure SSexhale @15:02:52.29 SS.seek 0ml @15:03:01.56 Exhaust.select :close @15:03:01.68 SSV.dial CBV @15:03:01.71
CBV.dial SSV @15:03:02.52 Allowing 130s for 25.0ml of remaining 125.0ml @15:03:02.53 Intake.select :open @15:03:05.23 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:03:05.24 SS.reconfigure SSinhale.with maxSpeed: 29 @15:03:05.30 SS.seek 25ml @15:04:05.40 Stroke completed -- waiting for pressure to equalize ... @15:04:09.28 Intake.select :close @15:04:09.44 SSV.dial :exhaust @15:04:10.22 Exhaust.select :open @15:04:10.33 SS.reconfigure SSexhale @15:04:10.38 SS.seek 0ml @15:04:19.43 Exhaust.select :close @15:04:19.55 SSV.dial CBV @15:04:19.58
CBV.dial SSV @15:04:20.38 Allowing 130s for 25.0ml of remaining 100.0ml @15:04:20.39 Intake.select :open @15:04:22.57 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:04:22.58 SS.reconfigure SSinhale.with maxSpeed: 29 @15:04:22.63 SS.seek 25ml @15:05:23.20 Stroke completed -- waiting for pressure to equalize ... @15:05:27.06 Intake.select :close @15:05:27.22 SSV.dial :exhaust @15:05:27.99 Exhaust.select :open @15:05:28.10 SS.reconfigure SSexhale @15:05:28.15 SS.seek 0ml @15:05:36.93 Exhaust.select :close @15:05:37.05 SSV.dial CBV @15:05:37.08
CBV.dial SSV @15:05:37.88 Allowing 130s for 25.0ml of remaining 75.0ml @15:05:37.89 Intake.select :open @15:05:40.06 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:05:40.08 SS.reconfigure SSinhale.with maxSpeed: 29 @15:05:40.13 SS.seek 25ml @15:06:40.99 Stroke completed -- waiting for pressure to equalize ... @15:06:45.47 Intake.select :close @15:06:45.63 SSV.dial :exhaust @15:06:46.42 Exhaust.select :open @15:06:46.53 SS.reconfigure SSexhale @15:06:46.58 SS.seek 0ml @15:06:55.37 Exhaust.select :close @15:06:55.49 SSV.dial CBV @15:06:55.52
CBV.dial SSV @15:06:56.33 Allowing 130s for 25.0ml of remaining 50.0ml @15:06:56.34 Intake.select :open @15:06:58.52 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:06:58.53 SS.reconfigure SSinhale.with maxSpeed: 29 @15:06:58.58 SS.seek 25ml @15:07:42.24 Vacuum too high -- waiting up to 40s @15:07:43.08 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:07:43.10 SS.reconfigure SSinhale.with maxSpeed: 26 @15:07:43.15 SS.seek 25ml @15:08:02.49 Stroke completed -- waiting for pressure to equalize ... @15:08:06.23 Intake.select :close @15:08:06.40 SSV.dial :exhaust @15:08:07.19 Exhaust.select :open @15:08:07.30 SS.reconfigure SSexhale @15:08:07.35 SS.seek 0ml @15:08:16.56 Exhaust.select :close @15:08:16.68 SSV.dial CBV @15:08:16.71
CBV.dial SSV @15:08:17.53 Allowing 130s for 25.0ml of remaining 25.0ml @15:08:17.54 Intake.select :open @15:08:19.72 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:08:19.74 SS.reconfigure SSinhale.with maxSpeed: 26 @15:08:19.79 SS.seek 24.97ml @15:09:26.87 Stroke completed -- waiting for pressure to equalize ... @15:09:30.76 Intake.select :close @15:09:30.92 SSV.dial :exhaust @15:09:31.69 Exhaust.select :open @15:09:31.80 SS.reconfigure SSexhale @15:09:31.85 SS.seek 0ml @15:09:41.07 Exhaust.select :close @15:09:41.18 Sampled 1500.0ml @15:09:41.20 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @15:09:41.21 Intake.select :close @15:09:41.28 CSV.dial :waste @15:09:41.36 CS.seek 0ml @15:09:41.45 CSV.dial :air @15:09:41.48 SSV.dial CBV,avoiding: :exhaust @15:09:41.52
CBV.dial SSV @15:09:42.31 CS.seek 10ml @15:09:43.46
CTV.dial :air @15:09:44.24 delay 15 seconds @15:09:59.27 SSV.dial :spit,avoiding: :exhaust @15:10:00.61 CTV.dial CSV,avoiding: :intake @15:10:00.66
SS.seek 0ml @15:10:00.80 SSV.dial CBV,avoiding: :exhaust @15:10:05.89 CBV.dial between SSV and waste2 @15:10:06.32 CTV.dial CSV,avoiding: :air @15:10:06.35
CSV.dial CTV @15:10:06.80 CS.reconfigure CSpressurize @15:10:06.86 CS.seek 0ml @15:10:32.47 BubblePoint: Intake @69.3psia, Outlet @13.6psia @15:10:32.48 CBV.dial SSV @15:10:32.83 SS.reconfigure SSconfig @15:10:32.88 SS.seek 10ml @15:10:38.50 delay 15 seconds @15:10:53.64 Evacuated: Intake @34.3psia, Outlet @-0.8psia @15:10:53.66 SSV.dial :spit,avoiding: :exhaust @15:10:55.00 SS.reconfigure SSexhale @15:10:55.05 SS.seek 0ml @15:10:59.98 SS.reconfigure SSfast @15:11:00.03 SS.seek 5ml @15:11:02.74 SSV.dial CBV,avoiding: :exhaust @15:11:02.77
CBV.dial SSV @15:11:04.13 CTV.dial :air,avoiding: :intake @15:11:05.46 CSV.dial CTV @15:11:05.51
SS.seek 10ml @15:11:06.50 CSV.dial :air @15:11:07.10 CS.reconfigure CSfast @15:11:07.15 CS.seek 0ml @15:11:08.24
SSV.dial :spit,avoiding: :exhaust @15:11:09.09 CS.coast @15:11:09.60
SS.reconfigure SSexhale @15:11:09.66 SS.seek 0ml @15:11:14.59 SS.reconfigure SSfast @15:11:14.64 SS.seek 5ml @15:11:17.33 SSV.dial CBV,avoiding: :exhaust @15:11:17.36
CBV.dial SSV @15:11:19.73 SSV.dial :spit,avoiding: :exhaust @15:11:21.10 SS.reconfigure SSconfig @15:11:21.15 SS.seek 0ml @15:11:24.25 SS.coast @15:11:24.29 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @15:11:24.30 Intake.select :close @15:11:24.38 CSV.dial :waste @15:11:24.53 SSV.dial CBV,avoiding: :exhaust @15:11:24.56
CBV.dial SSV @15:11:25.22 CS.seek 0ml @15:11:25.29 CSV.dial :air @15:11:25.91
CTV.dial :air @15:11:25.93 delay 15 seconds @15:11:26.05 CS.seek 10ml @15:11:40.95
SSV.dial :spit,avoiding: :exhaust @15:11:42.30 CTV.dial CSV,avoiding: :intake @15:11:42.40
SS.reconfigure SSexhale @15:11:42.45 SS.seek 0ml @15:11:42.64 SSV.dial CBV,avoiding: :exhaust @15:11:49.44 CBV.dial between SSV and waste2 @15:11:49.87 CSV.dial between air and CTV @15:11:50.31 CS.seek 8.282ml @15:11:55.19 CTV.dial CSV,avoiding: :air @15:11:55.22
CSV.dial CTV @15:11:55.57 CS.reconfigure CSpressurize @15:11:55.63 CS.seek 0ml @15:12:17.38 CSV.dial :air @15:12:17.86 CS.reconfigure CSfast @15:12:17.91 CS.seek 10ml @15:12:41.13 CSV.dial between air and CTV @15:12:41.48 CS.seek 3.128ml @15:12:57.79 CTV.dial CSV,avoiding: :air @15:12:57.82
CSV.dial CTV @15:12:58.14 CS.reconfigure CSpressurize @15:12:58.19 CS.seek 0ml @15:13:06.13 BubblePoint: Intake @68.1psia, Outlet @12.9psia @15:13:06.14 CBV.dial SSV @15:13:06.51 SS.reconfigure SSconfig @15:13:06.56 SS.seek 10ml @15:13:12.17 delay 15 seconds @15:13:27.32 Evacuated: Intake @37.8psia, Outlet @14.6psia @15:13:27.33 SSV.dial :spit,avoiding: :exhaust @15:13:28.69 SS.reconfigure SSexhale @15:13:28.75 SS.seek 0ml @15:13:33.92 SS.reconfigure SSfast @15:13:33.97 SS.seek 5ml @15:13:36.49 SSV.dial CBV,avoiding: :exhaust @15:13:36.52
CBV.dial SSV @15:13:37.88 CTV.dial :air,avoiding: :intake @15:13:39.72 CSV.dial CTV @15:13:39.77
SS.seek 10ml @15:13:40.76 CSV.dial :air @15:13:41.22 CS.reconfigure CSfast @15:13:41.28 CS.seek 0ml @15:13:42.24
SSV.dial :spit,avoiding: :exhaust @15:13:42.84 CS.coast @15:13:43.60
SS.reconfigure SSexhale @15:13:43.65 SS.seek 0ml @15:13:48.92 SS.reconfigure SSfast @15:13:48.98 SS.seek 5ml @15:13:51.42 SSV.dial CBV,avoiding: :exhaust @15:13:51.45
CBV.dial SSV @15:13:53.81 SSV.dial :spit,avoiding: :exhaust @15:13:55.16 SS.reconfigure SSconfig @15:13:55.21 SS.seek 0ml @15:13:58.34 SS.coast @15:13:58.39 Lysing: Adding 1.5ml lysis @15:13:58.40 CBV.dial between CSV and waste1,avoiding: :DBV @15:13:59.93 CTV.dial CSV @15:14:01.27 CSV.dial CSR @15:14:02.08 Pulling 1ml of CSR.lysis @15:14:02.09 CSR.select :lysis @15:14:02.22 CS.reconfigure CSconfig @15:14:02.26 CS.seek 1ml @15:14:06.01 delay 5 seconds @15:14:11.03 CSR.select :air @15:14:11.21 CS.seek 6ml @15:14:25.01 delay 2 seconds @15:14:27.03 CSV.dial :waste1 @15:14:28.33 CS.reconfigure CSfast @15:14:28.38 CS.seek :empty @15:14:42.78 CSV.dial CSR @15:14:44.16 Pulling 1.5ml of CSR.lysis @15:14:44.17 CSR.select :lysis @15:14:44.29 CS.reconfigure CSconfig @15:14:44.34 CS.seek 1.5ml @15:14:49.16 delay 5 seconds @15:14:54.18 CSR.select :air @15:14:54.37 CS.seek 4.501ml @15:15:03.07 delay 2 seconds @15:15:05.08 CSV.dial CTV @15:15:05.58 CS.seek 1.275ml @15:15:14.83 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @15:15:14.85 CH.seek 85C,8:00 @15:17:56.93 CH.hold 5:00,85C @15:22:57.67 Collection Puck Heater at 85C after lysing @15:22:57.69 CS.seek 0ml @15:23:02.01 CTV.dial between spare and air,avoiding: :intake @15:23:03.06 CSV.dial :air @15:23:03.52 CS.seek 5ml @15:23:17.42 CSV.dial CTV @15:23:17.89 delay 2 seconds @15:23:19.92 CSV.dial :air @15:23:20.40 delay 2 seconds @15:23:22.43 CSV.dial CTV @15:23:22.92 delay 2 seconds @15:23:24.93 CSV.dial :air @15:23:25.42 delay 2 seconds @15:23:27.45 CS.seek 0ml @15:23:41.20 Recovering Lysate into Collection syringe @15:23:41.21 CSV.dial CBV @15:23:42.23 CBV.dial CSV @15:23:42.63 CS.seek 5ml @15:23:56.33 delay 5 seconds @15:24:01.36 CTV.dial :air @15:24:01.91 CS.seek 7ml @15:24:08.14 delay 5 seconds @15:24:13.16 CSV.dial :air2 @15:24:13.63 CS.seek 10ml @15:24:22.45 CSV.dial between air and CTV,avoiding: CBV @15:24:23.73 Lysing Complete @15:24:23.81 Storing sh1 puck in Collection Clamp to tube 1 @15:24:24.01 SC.reconfigure SCtight @15:24:24.06 SC.seek 1 @15:24:26.22 SC.reconfigure SCloose @15:24:26.45 Elbow.reconfigure TightElbow @15:24:26.50 Elbow.seek 1 @15:24:28.43 Forearm.reconfigure TightForearm @15:24:28.48 Forearm.seek 1 + 700 counts @15:24:29.52 SE.reconfigure SEpick @15:24:29.59 SE.seek beamBreak @15:24:31.34 SE.configure SEconfig @15:24:31.51 Elbow.reconfigure LimpElbow @15:24:31.57 Forearm.reconfigure LooseForearm @15:24:31.62 Forearm.seek 1 @15:24:31.93 Elbow.hold @15:24:32.00 Elbow.reconfigure TightElbow @15:24:32.11 SE.seek 14.07 Pucks below Top Plate @15:24:32.61 Forearm.force -20 @15:24:32.65 Forearm.seek 1 + 331 counts @15:24:32.98 Forearm.reconfigure TightForearm @15:24:33.03 Forearm.seek 1 + 700 counts @15:24:33.31 SE.seek 15.07 Pucks below Top Plate @15:24:34.05 Forearm.reconfigure LooseForearm @15:24:34.10 Forearm.seek :retract @15:24:35.00 Elbow.seek CC @15:24:37.15 Elbow.reconfigure LooseElbow @15:24:37.26 Forearm.seek CC @15:24:38.19 Hand.close @15:24:38.85 CC.seek :open @15:24:43.34 Forearm.seek :retract @15:24:44.29 Elbow.reconfigure TightElbow @15:24:44.34 Elbow.seek 1 @15:24:46.25 Elbow.reconfigure LimpElbow @15:24:46.31 Forearm.seek 1 @15:24:47.34 Elbow.hold @15:24:47.42 Elbow.reconfigure TightElbow @15:24:47.47 Hand.open @15:24:48.21 Forearm.force -20 @15:24:48.26 Forearm.seek 1 + 349 counts @15:24:48.58 Forearm.reconfigure TightForearm @15:24:48.63 Forearm.seek 1 + 700 counts @15:24:49.03 SE.seek :down @15:24:50.19
Forearm.reconfigure LooseForearm @15:24:50.24 Forearm.seek :retract @15:24:50.34 SE.coast @15:24:51.17
SC.coast @15:24:51.21 Tranferring lysate to PRV @15:24:51.22 CSV.dial :air @15:24:51.57 CS.seek 10ml @15:24:51.64 CSV.dial between air and CTV @15:24:51.98 AIV.dial :bypass @15:24:52.19 Beginning samplePassC @15:24:52.20 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:24:52.28 Hand.open @15:24:52.72 Elbow.seek FlushPuck::Garage @15:24:54.78 Elbow.reconfigure LooseElbow @15:24:54.89 Forearm.seek :garage @15:24:55.90 Hand.close @15:24:56.71 Forearm.seek :retract @15:24:57.73 Elbow.reconfigure TightElbow @15:24:57.78 Elbow.seek CC @15:25:00.73 Elbow.reconfigure LooseElbow @15:25:00.84 Forearm.reconfigure TightForearm @15:25:00.89 Forearm.seek CC @15:25:02.20 CC.seek :closed @15:25:06.48 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.91 @15:25:06.56 Hand.open @15:25:07.27 Forearm.reconfigure LooseForearm @15:25:07.32 Forearm.seek :retract @15:25:08.31 AWV.select :air @15:25:08.32 AIV.dial :bypass @15:25:08.33 PSV.dial PSR @15:25:09.70 Pulling 1.5ml of PSR.flush @15:25:09.71 PSR.select :flush @15:25:09.85 PS.seek 1.5ml @15:25:25.83 delay 5 seconds @15:25:30.85 PSV.dial :waste1 @15:25:31.34 PS.seek 0ml @15:25:47.42 PSV.dial PSR @15:25:47.87 PSR.select :air @15:25:48.00 PS.seek 2.5ml @15:26:14.17 PRV.dial CBV @15:26:14.21
CBV.dial PRV @15:26:15.05 PSV.dial :mixloop @15:26:16.08 CTV.dial :air,avoiding: :intake @15:26:16.09 PTV.dial :PRVmixing @15:26:16.20 PS.seek 0ml @15:26:42.29 PSV.dial :air @15:26:42.79 PS.seek 2.5ml @15:27:08.94 PSV.dial :mixloop @15:27:09.42 PS.seek 0ml @15:27:35.57 PSV.dial :air @15:27:36.06 PS.seek 2.5ml @15:28:02.13 PSV.dial :mixloop @15:28:02.60 PS.seek 0ml @15:28:28.70 Garaging FlushPuck in Collection Clamp @15:28:28.78 Elbow.reconfigure TightElbow @15:28:28.83 Elbow.seek CC @15:28:29.07 Elbow.reconfigure LooseElbow @15:28:29.17 Forearm.seek CC @15:28:30.15 Hand.close @15:28:30.83 CC.seek :open @15:28:35.21 Forearm.seek :retract @15:28:36.19 Elbow.reconfigure TightElbow @15:28:36.24 Elbow.seek :garage @15:28:39.36 Elbow.reconfigure LooseElbow @15:28:39.47 Forearm.reconfigure TightForearm @15:28:39.52 Forearm.seek :garage @15:28:40.72 Hand.open @15:28:41.40 Forearm.reconfigure LooseForearm @15:28:41.45 Forearm.seek :retract @15:28:42.49 Connecting PS to CS to transfer sample @15:28:42.50 CBV.dial AIV @15:28:42.51 AIV.dial :bypass @15:28:42.52 PSV.dial :mixloop @15:28:42.54 PTV.dial :PRVmixing @15:28:42.55 PRV.dial AIV @15:28:42.56 CTV.dial :air,avoiding: :intake @15:28:42.58 Loading lyfil puck from tube 2 into Collection Clamp @15:28:42.85 SC.reconfigure SCtight @15:28:42.90 SC.seek 2 @15:28:45.05 SC.reconfigure SCloose @15:28:45.28 Elbow.reconfigure TightElbow @15:28:45.33 Elbow.seek 2 @15:28:47.94 Forearm.reconfigure TightForearm @15:28:47.99 Forearm.seek 2 + 700 counts @15:28:49.10 SE.reconfigure SEpick @15:28:49.18 SE.seek beamBreak @15:28:51.74 SE.configure SEconfig @15:28:51.91 Elbow.seek 2 @15:28:52.19 Forearm.seek 2 + 300 counts @15:28:52.39 Forearm.reconfigure LooseForearm @15:28:52.44 Hand.close @15:28:53.10 SE.seek :down @15:28:53.32
Forearm.seek :retract @15:28:54.25 Elbow.seek CC @15:28:55.45 SE.coast @15:28:55.93
Elbow.reconfigure LooseElbow @15:28:56.04 Forearm.reconfigure TightForearm @15:28:56.09 Forearm.seek CC @15:28:57.36 CC.seek :closed @15:29:01.64 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 1.91 @15:29:01.65 Hand.open @15:29:02.41 Forearm.reconfigure LooseForearm @15:29:02.46 Forearm.seek :retract @15:29:03.43 SC.coast @15:29:03.47 CTV.dial CSV,avoiding: :intake @15:29:04.82 CSV.dial CTV @15:29:05.14 CS.seek 5ml @15:29:18.93 delay 1:20 @15:30:38.96 CS.seek 2.5ml @15:30:46.43 delay 40 seconds @15:31:26.46 CS.seek 0ml @15:31:33.80 delay 1:00 @15:32:33.82 PSV.dial :air @15:32:34.29 PS.seek 2.5ml @15:33:00.48 PSV.dial :mixloop @15:33:00.96 delay 3 seconds @15:33:03.98 PSV.dial :air @15:33:04.46 delay 2 seconds @15:33:06.48 PSV.dial :mixloop @15:33:06.96 delay 3 seconds @15:33:09.98 PSV.dial :waste1 @15:33:11.32 PS.seek :empty @15:33:37.48 CBV.dial :waste1 @15:33:37.96 delay 5 seconds @15:33:42.98 CBV.dial AIV @15:33:43.48 delay 10 seconds @15:33:53.50 CBV.dial :waste1 @15:33:54.01 delay 10 seconds @15:34:04.03 CBV.dial AIV @15:34:04.54 delay 10 seconds @15:34:14.57 CTV.dial CSV,avoiding: :intake @15:34:14.58 CBV.dial between AIV and waste1 @15:34:14.94 CSV.dial :air @15:34:15.42 CS.seek 10ml @15:34:41.89 CSV.dial CTV @15:34:42.39 CS.seek 0ml @15:35:08.92 CBV.dial :waste1 @15:35:09.24 delay 10 seconds @15:35:19.27 CTV.dial :air,avoiding: :intake @15:35:21.20 Storing lyfil puck in Collection Clamp to tube 1 @15:35:21.40 SC.reconfigure SCtight @15:35:21.45 SC.seek 1 @15:35:23.59 SC.reconfigure SCloose @15:35:23.82 Elbow.reconfigure TightElbow @15:35:23.87 Elbow.seek 1 @15:35:25.76 Forearm.reconfigure TightForearm @15:35:25.81 Forearm.seek 1 + 700 counts @15:35:26.86 SE.reconfigure SEpick @15:35:26.93 SE.seek beamBreak @15:35:28.63 SE.configure SEconfig @15:35:28.80 Elbow.reconfigure LimpElbow @15:35:28.86 Forearm.reconfigure LooseForearm @15:35:28.91 Forearm.seek 1 @15:35:29.23 Elbow.hold @15:35:29.30 Elbow.reconfigure TightElbow @15:35:29.41 SE.seek 15.09 Pucks below Top Plate @15:35:29.87 Forearm.force -20 @15:35:29.91 Forearm.seek 1 + 328 counts @15:35:30.23 Forearm.reconfigure TightForearm @15:35:30.28 Forearm.seek 1 + 700 counts @15:35:30.62 SE.seek 16.09 Pucks below Top Plate @15:35:31.31 Forearm.reconfigure LooseForearm @15:35:31.36 Forearm.seek :retract @15:35:32.31 Elbow.seek CC @15:35:33.93 Elbow.reconfigure LooseElbow @15:35:34.04 Forearm.seek CC @15:35:35.00 Hand.close @15:35:35.59 CC.seek :open @15:35:40.10 Forearm.seek :retract @15:35:41.06 Elbow.reconfigure TightElbow @15:35:41.11 Elbow.seek 1 @15:35:42.99 Elbow.reconfigure LimpElbow @15:35:43.05 Forearm.seek 1 @15:35:44.06 Elbow.hold @15:35:44.13 Elbow.reconfigure TightElbow @15:35:44.18 Hand.open @15:35:44.98 Forearm.force -20 @15:35:45.02 Forearm.seek 1 + 342 counts @15:35:45.34 Forearm.reconfigure TightForearm @15:35:45.39 Forearm.seek 1 + 700 counts @15:35:45.80 SE.seek :down @15:35:46.95
Forearm.reconfigure LooseForearm @15:35:47.00 Forearm.seek :retract @15:35:47.06 SE.coast @15:35:47.95
SC.coast @15:35:47.99 PSV.dial :air @15:35:49.09 PS.seek 0.499ml @15:35:55.10 delay 3 seconds @15:35:58.11 PSV.dial :mixloop @15:35:58.57 PRV.dial :air @15:35:59.05 delay 3 seconds @15:36:02.07 PRV.dial CBV @15:36:02.55 CBV.dial AIV @15:36:03.02 PRV.dial between CBV and air @15:36:03.38 PRV.dial :air @15:36:03.78 PS.reconfigure PSslow @15:36:03.83 PS.seek 0.599ml @15:36:06.39 PRV.dial :waste2 @15:36:07.15 PS.reconfigure PSconfig @15:36:07.20 PS.seek :empty @15:36:14.12 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:36:14.20 Hand.open @15:36:14.61 Elbow.seek FlushPuck::Garage @15:36:16.63 Elbow.reconfigure LooseElbow @15:36:16.73 Forearm.seek :garage @15:36:17.76 Hand.close @15:36:18.48 Forearm.seek :retract @15:36:19.52 Elbow.reconfigure TightElbow @15:36:19.57 Elbow.seek CC @15:36:22.42 Elbow.reconfigure LooseElbow @15:36:22.52 Forearm.reconfigure TightForearm @15:36:22.57 Forearm.seek CC @15:36:23.87 CC.seek :closed @15:36:28.15 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.88 @15:36:28.23 Hand.open @15:36:28.91 Forearm.reconfigure LooseForearm @15:36:28.96 Forearm.seek :retract @15:36:30.03 Rinsing MixLoop with GuSCN @15:36:30.04 PSV.dial :mixloop,avoiding: PBV @15:36:30.06 PTV.dial :PRVmixing,avoiding: :puck @15:36:30.07 PRV.dial :spare1 @15:36:31.19 PS.seek 1.099ml @15:36:43.24 delay 2 seconds @15:36:45.25 PRV.dial :air @15:36:46.30 PS.seek 2.5ml @15:37:01.30 PSV.dial :waste1,avoiding: PBV @15:37:02.60 PS.seek :empty @15:37:28.71 Diluting Lysate in Syringe @15:37:28.72 PSV.dial :PTV,avoiding: PBV @15:37:30.32 PTV.dial :PRV,avoiding: :puck @15:37:31.07 CBV.dial :PRV @15:37:31.08 PRV.dial :air @15:37:31.09 delay 2 seconds @15:37:33.11 PRV.dial CBV @15:37:33.59 delay 2 seconds @15:37:35.61 PS.reconfigure PSslow1 @15:37:35.66 PS.seek 0.1ml @15:37:46.52 delay 5 seconds @15:37:51.54 PRV.dial :spare1 @15:37:52.30 PS.reconfigure PSconfig @15:37:52.35 PS.seek 1ml @15:38:02.30 delay 2 seconds @15:38:04.82 PRV.dial :air @15:38:05.91 PS.seek 2.5ml @15:38:21.93 delay 2 seconds @15:38:23.95 Parking diluted lysate in Mixing Coil @15:38:23.96 PSV.dial :mixloop @15:38:24.43 PTV.dial :PRVmixing,avoiding: :puck @15:38:25.18 PRV.dial :CBV @15:38:25.73 PS.seek 1.5ml @15:38:36.74 delay 2 seconds @15:38:38.76 PSV.dial :waste1,avoiding: PBV @15:38:40.09 PS.seek :empty @15:38:56.09 Beginning samplePassF @15:38:56.17 AWV.select :air @15:38:56.18 AIV.dial :bypass @15:38:56.20 PSV.dial PSR,avoiding: PBV @15:38:56.72 Pulling 1.5ml of PSR.flush @15:38:56.73 PSR.select :flush @15:38:56.86 PS.seek 1.5ml @15:39:12.99 delay 5 seconds @15:39:18.02 PSV.dial :waste1 @15:39:18.50 PS.seek 0ml @15:39:34.51 PSV.dial PSR @15:39:34.98 PSR.select :air @15:39:35.11 PS.seek 2.5ml @15:40:01.21 PRV.dial CBV @15:40:01.24
CBV.dial PRV @15:40:01.25 PSV.dial :PTV,via: :air @15:40:03.13 CTV.dial :air,avoiding: :intake @15:40:03.14 PTV.dial :PRV,avoiding: :puck @15:40:03.91 PS.seek 0ml @15:40:30.11 PSV.dial :air @15:40:30.88 PS.seek 2.5ml @15:40:57.01 PSV.dial :PTV @15:40:57.77 PS.seek 0ml @15:41:23.91 moving diluted lysate into CBV-AIV line @15:41:23.92 PSV.dial :air @15:41:24.67 PS.seek 2.5ml @15:41:50.74 delay 2 seconds @15:41:52.75 PSV.dial :mixloop @15:41:53.21 PTV.dial :PRVmixing,avoiding: :puck @15:41:53.96 PRV.dial CBV @15:41:54.05 PS.seek 1.8ml @15:42:01.96 delay 3 seconds @15:42:04.98 PRV.dial :waste2 @15:42:06.03 PS.seek :empty @15:42:25.03 Clearing PML for modified lysate @15:42:25.04 CTV.dial :air,avoiding: :intake @15:42:25.05 PRV.dial :waste2 @15:42:25.07 PTV.dial :PRV,avoiding: :puck @15:42:25.84 delay 2 seconds @15:42:27.87 PTV.dial :PRVmixing,avoiding: :puck @15:42:28.63 delay 2 seconds @15:42:30.66 PSV.dial :PML @15:42:30.69 delay 2 seconds @15:42:32.70 PRV.dial :air @15:42:33.46 delay 2 seconds @15:42:35.54 PS.seek 1.499ml @15:42:51.56 delay 5 seconds @15:42:56.59 PS.seek 2.5ml @15:43:07.58 PSV.dial :air @15:43:08.06 PS.seek 0ml @15:43:34.18 mixing lysate with speDil @15:43:34.19 PSV.dial :PML @15:43:34.65 CBV.dial :PRV @15:43:34.66 PRV.dial :air @15:43:34.67 delay 2 seconds @15:43:36.69 PRV.dial :speDil @15:43:37.18 delay 2 seconds @15:43:39.27 PS.reconfigure PSslow1 @15:43:39.32 PS.seek 0.225ml @15:44:02.96 delay 5 seconds @15:44:07.98 PRV.dial CBV @15:44:08.76 delay 2 seconds @15:44:10.85 PS.seek 0.475ml @15:44:36.53 delay 5 seconds @15:44:41.55 PRV.dial :air @15:44:42.54 delay 2 seconds @15:44:44.58 PS.seek 0.965ml @15:45:34.50 delay 10 seconds @15:45:44.53 PRV.dial CBV @15:45:45.00 CTV.dial :air,avoiding: :intake @15:45:45.01 PSV.dial :air @15:45:45.48 PS.reconfigure PSconfig @15:45:45.53 PS.seek 2.5ml @15:46:01.90 delay 3 seconds @15:46:04.92 PSV.dial PTV @15:46:05.79 PTV.dial :PRV,avoiding: :puck @15:46:06.55 PS.seek 0ml @15:46:32.74 PSV.dial :air @15:46:33.49 PS.seek 2.5ml @15:46:59.56 delay 3 seconds @15:47:02.58 PSV.dial PTV @15:47:03.32 PTV.dial :PRV,avoiding: :puck @15:47:03.34 PS.seek 0ml @15:47:29.53 Pushing modified lysate into AIV Injection Loop @15:47:29.54 CBV.dial AIV @15:47:29.55 AIV.dial :bypass @15:47:29.57 PRV.dial CBV @15:47:29.58 PSV.dial :air @15:47:30.34 PS.seek 2.5ml @15:47:56.47 delay 3 seconds @15:47:59.48 PSV.dial :PML @15:47:59.95 PTV.dial :PRVmixing,avoiding: :puck @15:48:00.77 PS.reconfigure PSslow1 @15:48:00.82 PS.seek 1.74ml @15:49:18.40 delay 5 seconds @15:49:23.42 AIV.dial :sample @15:49:23.99 AWV.select :waste @15:49:24.16 PS.reconfigure PSslow2 @15:49:24.21 PS.seek 1.22ml @15:51:12.69 delay 15 seconds @15:51:27.72 AIV.dial :bypass @15:51:28.29 PS.reconfigure PSconfig @15:51:28.34 PS.seek 0ml @15:51:41.49 PSV.dial :air @15:51:41.98 PS.seek 2.5ml @15:52:08.18 PSV.dial :PML @15:52:08.67 PS.seek 0ml @15:52:34.77 AIV.dial :coilToMFB @15:52:34.79 AWV.select :air @15:52:34.96 Extracting Nucleic Acid from AIV @15:52:34.98
Beginning samplePassD @15:52:35.02 SPE.hold 2:46:40,55C @15:52:35.13 ASV.dial :SPEdelivery @15:52:35.20
AWV.select :air @15:52:35.22 AIV.dial :bypass @15:52:35.23 PSV.dial PSR @15:52:35.52 delay 20 seconds @15:52:36.37
Pulling 1.5ml of PSR.killMFB @15:52:36.38 PSR.select :killMFB @15:52:36.51 PS.seek 1.5ml @15:52:52.53 delay 5 seconds @15:52:55.55 primeLysateINT @15:52:55.56 AWV.select :air @15:52:55.57 AIV.dial :coilToMFB @15:52:55.59 ATV.dial AIV @15:52:57.20 AS.reconfigure ASslow1 @15:52:57.25 AS.seek 0.299ml @15:52:57.55
PSV.dial :waste1 @15:52:58.04 PS.seek 0ml @15:53:13.99 PSV.dial PSR @15:53:14.47 PSR.select :air @15:53:14.60 PS.seek 2.5ml @15:53:40.75 PRV.dial CBV @15:53:40.78
CBV.dial PRV @15:53:40.79 PSV.dial :mixloop @15:53:41.99 CTV.dial :air,avoiding: :intake @15:53:42.00 PTV.dial :PRVmixing @15:53:42.02 PS.seek 0ml @15:53:57.94 delay 10 seconds @15:54:07.96 ATV.dial :air2 @15:54:08.34
PSV.dial PSR @15:54:09.00 AS.seek 0.329ml @15:54:09.98
Pulling 1.5ml of PSR.flush @15:54:09.99 PSR.select :flush @15:54:10.12 PS.seek 1.5ml @15:54:15.34 ATV.dial :waste2 @15:54:15.88 AS.reconfigure ASnorm @15:54:15.94 AS.seek :empty @15:54:26.13
delay 5 seconds @15:54:31.15 PSV.dial :waste1 @15:54:31.65 PS.seek 0ml @15:54:32.93 Lysate primed! @15:54:32.94 AFV.select :holdingCoil @15:54:32.95 ATV.dial :air2 @15:54:33.55 AS.reconfigure ASslow1 @15:54:33.60 AS.seek 0.069ml @15:54:47.72
PSV.dial PSR @15:54:48.04 delay 3 seconds @15:54:48.20
PSR.select :air @15:54:48.33 PS.seek 2.5ml @15:54:51.06 ATV.dial :AIV @15:54:51.97 AS.seek 0.469ml @15:55:14.53 PRV.dial CBV @15:55:14.56
CBV.dial PRV @15:55:14.57 PSV.dial :mixloop @15:55:15.63 CTV.dial :air,avoiding: :intake @15:55:15.64 PTV.dial :PRVmixing @15:55:15.66 PS.seek 0ml @15:55:41.78 PSV.dial PSR @15:55:42.89 Pulling 1.5ml of PSR.flush @15:55:42.90 PSR.select :flush @15:55:43.03 PS.seek 1.5ml @15:55:59.16 delay 5 seconds @15:56:04.19 PSV.dial :waste1 @15:56:04.67 PS.seek 0ml @15:56:12.68 delay 5 seconds @15:56:17.77 Solid Phase Extraction Heater at 50.9C @15:56:17.78 ATV.dial ASV @15:56:18.16 ASV.dial :SPEdelivery @15:56:18.24 AS.seek 0.041ml @15:56:20.82
PSV.dial PSR @15:56:21.30 PSR.select :air @15:56:21.43 PS.seek 2.5ml @15:56:47.60 PRV.dial CBV @15:56:47.63
CBV.dial PRV @15:56:47.65 PSV.dial :mixloop @15:56:48.69 CTV.dial :air,avoiding: :intake @15:56:48.70 PTV.dial :PRVmixing @15:56:48.72 PS.seek 0ml @15:57:14.85 PSV.dial PSR @15:57:15.95 Pulling 1.5ml of PSR.flush @15:57:15.96 PSR.select :flush @15:57:16.09 PS.seek 1.5ml @15:57:32.10 delay 5 seconds @15:57:37.13 PSV.dial :waste1 @15:57:37.60 PS.seek 0ml @15:57:44.60 delay 10 seconds @15:57:53.65
PSV.dial PSR @15:57:54.14 PSR.select :air @15:57:54.27 PS.seek 2.5ml @15:57:54.62 rinseLysateLineINT @15:57:54.63 AFV.select :carrierSolution @15:57:54.74 delay 2 seconds @15:57:56.77 AS.reconfigure ASconfig @15:57:56.82 AS.seek 1ml @15:58:20.38 PRV.dial CBV @15:58:20.41
CBV.dial PRV @15:58:20.42 PSV.dial :mixloop @15:58:21.46 CTV.dial :air,avoiding: :intake @15:58:21.47 PTV.dial :PRVmixing @15:58:21.49 PS.reconfigure PSslow @15:58:21.55 PS.seek 1.2ml @15:58:48.32 PSV.dial :waste1 @15:58:49.66 PS.reconfigure PSconfig @15:58:49.71 PS.seek 0ml @15:58:52.23 delay 1 second @15:58:53.24 AFV.select :holdingCoil @15:58:53.35 delay 1 second @15:58:54.38 ATV.dial AIV @15:58:55.43 AIV.dial :coilToMFB @15:58:55.44 AWV.select :waste @15:58:55.56 AS.reconfigure ASslow0 @15:58:55.61 AS.seek 0ml @15:59:02.72
PSV.dial :air @15:59:03.76 PS.seek 2.5ml @15:59:29.97 PSV.dial :mixloop @15:59:30.41 PS.reconfigure PSslow @15:59:30.46 PS.seek 0ml @16:00:21.57 PSV.dial :air @16:00:22.04 PS.reconfigure PSconfig @16:00:22.09 PS.seek 2.5ml @16:00:36.52 ATV.dial :air2 @16:00:37.17 AS.reconfigure ASslow1 @16:00:37.22 AS.seek 0.05ml @16:00:47.59 ATV.dial :AIV @16:00:48.21
PSV.dial :mixloop @16:00:48.38 AS.seek 0.01ml @16:00:48.68
PS.reconfigure PSslow @16:00:48.73 PS.seek 0ml @16:00:56.73 ATV.dial :waste @16:00:58.35 AS.seek :empty @16:01:00.81 ethanol wash @16:01:00.82 ATV.dial :air2 @16:01:02.01 AS.reconfigure ASslow2 @16:01:02.06 AS.seek 0.009ml @16:01:12.48 delay 3 seconds @16:01:15.51 Pulling 0.4ml of Ethanol Wash @16:01:15.52 ATV.dial :ethanol @16:01:16.30 AS.reconfigure ASslow1 @16:01:16.35 AS.seek 0.409ml @16:01:39.94
Cleaning Collection Syringe @16:01:39.95 CSV.dial CSR @16:01:40.47 Pulling 4ml of CSR.flush @16:01:40.48 CSR.select :flush @16:01:40.61 CS.seek 4ml @16:01:51.80 delay 5 seconds @16:01:56.82 CSR.select :air @16:01:56.96 CS.seek 10ml @16:02:13.30 CSV.dial CTV @16:02:13.33
CTV.dial CSV @16:02:14.73 CBV.dial :waste1 @16:02:15.23 CS.seek 0ml @16:02:37.09 delay 3 seconds @16:02:40.11 ATV.dial :air1 @16:02:40.49 delay 3 seconds @16:02:41.79
CSV.dial CSR @16:02:43.11 Pulling 4ml of CSR.flush @16:02:43.12 CSR.select :flush @16:02:43.25 CS.seek 4ml @16:02:43.58 AS.seek 0.434ml @16:02:48.88 delay 3 seconds @16:02:51.90 ATV.dial :ethanol @16:02:52.50 AS.seek 0.419ml @16:02:54.42
delay 5 seconds @16:02:55.82 delay 3 seconds @16:02:58.84 ATV.dial ASV @16:02:59.44
CSR.select :air @16:02:59.58 CS.seek 10ml @16:03:00.10 AS.seek 0.092ml @16:03:15.92 CSV.dial CTV @16:03:15.95
CTV.dial CSV @16:03:16.45 CBV.dial :waste1 @16:03:16.47 CS.seek 0ml @16:03:43.09 Garaging FlushPuck in Collection Clamp @16:03:43.16 Elbow.reconfigure TightElbow @16:03:43.21 Elbow.seek CC @16:03:43.47 Elbow.reconfigure LooseElbow @16:03:43.57 Forearm.seek CC @16:03:44.56 Hand.close @16:03:45.13 CC.seek :open @16:03:50.03 Forearm.seek :retract @16:03:51.04 Elbow.reconfigure TightElbow @16:03:51.09 Elbow.seek :garage @16:03:54.14 Elbow.reconfigure LooseElbow @16:03:54.25 Forearm.reconfigure TightForearm @16:03:54.30 Forearm.seek :garage @16:03:55.47 Hand.open @16:03:56.20 Forearm.reconfigure LooseForearm @16:03:56.25 Forearm.seek :retract @16:03:57.27 Whole Cell Archive sampling 1500ml @16:03:57.29 Loading wcr puck from tube 2 into Collection Clamp @16:03:57.56 SC.reconfigure SCtight @16:03:57.61 SC.seek 2 @16:03:59.81 SC.reconfigure SCloose @16:04:00.05 Elbow.reconfigure TightElbow @16:04:00.10 Elbow.seek 2 @16:04:02.21 Forearm.reconfigure TightForearm @16:04:02.27 Forearm.seek 2 + 700 counts @16:04:03.36 SE.reconfigure SEpick @16:04:03.44 SE.seek beamBreak @16:04:06.16 SE.configure SEconfig @16:04:06.24 delay 3 seconds @16:04:06.40 Elbow.seek 2 @16:04:06.61 Forearm.seek 2 + 300 counts @16:04:06.91 Forearm.reconfigure LooseForearm @16:04:06.96 Hand.close @16:04:07.66 SE.seek :down @16:04:07.90 Forearm.seek :retract @16:04:08.83 Elbow.seek CC @16:04:09.27 ATV.dial :waste2 @16:04:09.99 AS.reconfigure ASconfig @16:04:10.04 AS.seek :empty @16:04:10.24 SE.coast @16:04:10.47 Elbow.reconfigure LooseElbow @16:04:10.59 Forearm.reconfigure TightForearm @16:04:10.64 Forearm.seek CC @16:04:11.95 CC.seek :closed @16:04:15.70 ATV.dial :air2 @16:04:16.10 AS.reconfigure ASslow1 @16:04:16.24 AS.seek 1ml @16:04:16.29 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.91 @16:04:16.32 Hand.open @16:04:17.62 Forearm.reconfigure LooseForearm @16:04:17.70 Forearm.seek :retract @16:04:18.69 SC.coast @16:04:18.73 collectSample 1500ml w/bubble point of 55psi @16:04:18.75 Priming 7ml @16:04:18.76 Intake.select :close @16:04:18.77 Exhaust.select :close @16:04:18.79 SS.hold @16:04:19.03 Calibrating pressure sensors @16:04:19.04 CTV.dial :air @16:04:20.38 SSV.dial :spit,avoiding: :exhaust @16:04:20.41 SS.reconfigure SSfast @16:04:20.46 SS.seek 5ml @16:04:23.10 SSV.dial CBV @16:04:23.13 CBV.dial SSV @16:04:24.56 delay 3.8 seconds @16:04:28.51 SSV.dial :spit @16:04:29.89 SS.seek 0ml @16:04:32.52 SSV.dial CBV @16:04:33.90 CTV.dial :intake @16:04:34.69 SSV.dial CBV @16:04:34.72 CBV.dial SSV @16:04:34.75 Allowing 40s for 7.0ml of remaining 7.0ml @16:04:34.76 Intake.select :open @16:04:36.95 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:04:36.97 SS.reconfigure SSinhale.with maxSpeed: 150 @16:04:37.02 SS.seek 7ml @16:04:41.68 Vacuum too high -- waiting up to 40s @16:04:44.86 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:04:44.88 SS.reconfigure SSinhale.with maxSpeed: 135 @16:04:44.93 SS.seek 7ml @16:04:46.01 Stroke completed -- waiting for pressure to equalize ... @16:04:53.04 Intake.select :close @16:04:53.21 SSV.dial :exhaust @16:04:54.00 Exhaust.select :open @16:04:54.12 SS.reconfigure SSexhale @16:04:54.18 SS.seek 0ml @16:04:57.82 Exhaust.select :close @16:04:57.93 Sampling 1500.0ml @16:04:57.95 Intake.select :close @16:04:57.96 Exhaust.select :close @16:04:57.97 SS.reconfigure SSconfig @16:04:58.02 SS.hold @16:04:58.25 CTV.dial :intake @16:04:58.27 SSV.dial CBV @16:04:58.83 CBV.dial SSV @16:04:59.69 Allowing 130s for 25.0ml of remaining 1500.0ml @16:04:59.70 Intake.select :open @16:05:01.89 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:05:01.91 SS.reconfigure SSinhale.with maxSpeed: 135 @16:05:01.96 SS.seek 25ml @16:05:05.67 Vacuum too high -- waiting up to 40s @16:05:07.05 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:05:07.07 SS.reconfigure SSinhale.with maxSpeed: 122 @16:05:07.12 SS.seek 25ml @16:05:09.49 Vacuum too high -- waiting up to 40s @16:05:11.11 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:05:11.13 SS.reconfigure SSinhale.with maxSpeed: 110 @16:05:11.19 SS.seek 25ml @16:05:13.90 Vacuum too high -- waiting up to 40s @16:05:15.26 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:05:15.28 SS.reconfigure SSinhale.with maxSpeed: 99 @16:05:15.33 SS.seek 25ml @16:05:18.05 Vacuum too high -- waiting up to 40s @16:05:19.68 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:05:19.70 SS.reconfigure SSinhale.with maxSpeed: 89 @16:05:19.75 SS.seek 25ml @16:05:22.77 Vacuum too high -- waiting up to 40s @16:05:24.15 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:05:24.17 SS.reconfigure SSinhale.with maxSpeed: 80 @16:05:24.22 SS.seek 25ml @16:05:27.62 Vacuum too high -- waiting up to 40s @16:05:28.99 Intake @33.5psia, vacuum from 20.0 to 24.0psi @16:05:29.01 SS.reconfigure SSinhale.with maxSpeed: 72 @16:05:29.07 SS.seek 25ml @16:05:33.66 Vacuum too high -- waiting up to 40s @16:05:35.60 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:05:35.63 SS.reconfigure SSinhale.with maxSpeed: 65 @16:05:35.68 SS.seek 25ml @16:05:39.90 Stroke completed -- waiting for pressure to equalize ... @16:05:43.15 Intake.select :close @16:05:43.31 SSV.dial :exhaust @16:05:44.11 Exhaust.select :open @16:05:44.23 SS.reconfigure SSexhale @16:05:44.29 SS.seek 0ml @16:05:53.00 Exhaust.select :close @16:05:53.11 SSV.dial CBV @16:05:53.14 CBV.dial SSV @16:05:53.95 Allowing 130s for 25.0ml of remaining 1475.0ml @16:05:53.96 Intake.select :open @16:05:56.15 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:05:56.17 SS.reconfigure SSinhale.with maxSpeed: 65 @16:05:56.22 SS.seek 25ml @16:06:02.54 Vacuum too high -- waiting up to 40s @16:06:03.67 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:06:03.69 SS.reconfigure SSinhale.with maxSpeed: 59 @16:06:03.74 SS.seek 25ml @16:06:12.27 Vacuum too high -- waiting up to 40s @16:06:13.38 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:06:13.40 SS.reconfigure SSinhale.with maxSpeed: 53 @16:06:13.45 SS.seek 25ml @16:06:31.69 Stroke completed -- waiting for pressure to equalize ... @16:06:34.93 Intake.select :close @16:06:35.10 SSV.dial :exhaust @16:06:35.89 Exhaust.select :open @16:06:36.00 SS.reconfigure SSexhale @16:06:36.06 SS.seek 0ml @16:06:44.80 Exhaust.select :close @16:06:44.92 SSV.dial CBV @16:06:44.95 CBV.dial SSV @16:06:45.77 Allowing 130s for 25.0ml of remaining 1450.0ml @16:06:45.78 Intake.select :open @16:06:47.96 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:06:47.98 SS.reconfigure SSinhale.with maxSpeed: 53 @16:06:48.03 SS.seek 25ml @16:07:14.61 Vacuum too high -- waiting up to 40s @16:07:15.48 Intake @33.4psia, vacuum from 20.0 to 24.0psi @16:07:15.50 SS.reconfigure SSinhale.with maxSpeed: 48 @16:07:15.55 SS.seek 25ml @16:07:24.16 Stroke completed -- waiting for pressure to equalize ... @16:07:26.77 Intake.select :close @16:07:26.93 SSV.dial :exhaust @16:07:28.30 Exhaust.select :open @16:07:28.41 SS.reconfigure SSexhale @16:07:28.47 SS.seek 0ml @16:07:37.19 Exhaust.select :close @16:07:37.30 SSV.dial CBV @16:07:37.32 CBV.dial SSV @16:07:37.90 delay 5 seconds @16:07:38.14 Allowing 130s for 25.0ml of remaining 1425.0ml @16:07:38.15 Intake.select :open @16:07:40.33 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:07:40.36 SS.reconfigure SSinhale.with maxSpeed: 48 @16:07:40.41 SS.seek 25ml @16:07:42.92 ATV.dial ASV @16:07:43.96 ASV.dial :SPEdelivery @16:07:43.98 AS.seek 0.02ml @16:08:17.98 Stroke completed -- waiting for pressure to equalize ... @16:08:20.59 Intake.select :close @16:08:20.76 SSV.dial :exhaust @16:08:21.56 Exhaust.select :open @16:08:21.68 SS.reconfigure SSexhale @16:08:21.74 SS.seek 0ml @16:08:30.44 Exhaust.select :close @16:08:30.56 SSV.dial CBV @16:08:30.59 CBV.dial SSV @16:08:31.41 Allowing 130s for 25.0ml of remaining 1400.0ml @16:08:31.42 Intake.select :open @16:08:33.61 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:08:33.63 SS.reconfigure SSinhale.with maxSpeed: 48 @16:08:33.68 SS.seek 25ml @16:09:11.18 Stroke completed -- waiting for pressure to equalize ... @16:09:13.79 Intake.select :close @16:09:13.96 SSV.dial :exhaust @16:09:14.75 Exhaust.select :open @16:09:14.87 SS.reconfigure SSexhale @16:09:14.93 SS.seek 0ml @16:09:24.08 Exhaust.select :close @16:09:24.19 SSV.dial CBV @16:09:24.22 CBV.dial SSV @16:09:25.03 Allowing 130s for 25.0ml of remaining 1375.0ml @16:09:25.04 Intake.select :open @16:09:27.22 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:09:27.24 SS.reconfigure SSinhale.with maxSpeed: 48 @16:09:27.29 SS.seek 25ml @16:10:04.54 Stroke completed -- waiting for pressure to equalize ... @16:10:07.15 Intake.select :close @16:10:07.31 SSV.dial :exhaust @16:10:08.11 Exhaust.select :open @16:10:08.22 SS.reconfigure SSexhale @16:10:08.28 SS.seek 0ml @16:10:17.26 Exhaust.select :close @16:10:17.38 SSV.dial CBV @16:10:17.41 CBV.dial SSV @16:10:18.24 Allowing 130s for 25.0ml of remaining 1350.0ml @16:10:18.25 Intake.select :open @16:10:20.44 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:10:20.46 SS.reconfigure SSinhale.with maxSpeed: 48 @16:10:20.51 SS.seek 25ml @16:10:57.65 Stroke completed -- waiting for pressure to equalize ... @16:11:00.26 Intake.select :close @16:11:00.92 SSV.dial :exhaust @16:11:01.43 delay 5 seconds @16:11:01.72 Exhaust.select :open @16:11:01.84 SS.reconfigure SSexhale @16:11:01.90 SS.seek 0ml @16:11:06.44 ATV.dial :air2 @16:11:07.43 AS.seek 0.52ml @16:11:11.10 Exhaust.select :close @16:11:11.22 SSV.dial CBV @16:11:11.25 CBV.dial SSV @16:11:12.08 Allowing 130s for 25.0ml of remaining 1325.0ml @16:11:12.09 Intake.select :open @16:11:14.27 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:11:14.29 SS.reconfigure SSinhale.with maxSpeed: 48 @16:11:14.34 SS.seek 25ml @16:11:51.60 Stroke completed -- waiting for pressure to equalize ... @16:11:54.86 Intake.select :close @16:11:55.03 SSV.dial :exhaust @16:11:55.83 Exhaust.select :open @16:11:55.95 SS.reconfigure SSexhale @16:11:56.01 SS.seek 0ml @16:12:04.81 Exhaust.select :close @16:12:04.92 SSV.dial CBV @16:12:04.95 CBV.dial SSV @16:12:05.77 Allowing 130s for 25.0ml of remaining 1300.0ml @16:12:05.78 Intake.select :open @16:12:07.96 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:12:07.98 SS.reconfigure SSinhale.with maxSpeed: 48 @16:12:08.03 SS.seek 25ml @16:12:45.49 Stroke completed -- waiting for pressure to equalize ... @16:12:48.25 delay 5 seconds @16:12:48.75 Intake.select :close @16:12:48.92 SSV.dial :exhaust @16:12:49.72 Exhaust.select :open @16:12:49.83 SS.reconfigure SSexhale @16:12:49.89 SS.seek 0ml @16:12:53.27 ATV.dial ASV @16:12:54.20 ASV.dial :wasteR @16:12:54.57 AS.seek 0.3ml @16:12:58.90 Exhaust.select :close @16:12:59.01 SSV.dial CBV @16:12:59.04 CBV.dial SSV @16:12:59.85 Allowing 130s for 25.0ml of remaining 1275.0ml @16:12:59.86 Intake.select :open @16:13:02.05 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:13:02.07 SS.reconfigure SSinhale.with maxSpeed: 48 @16:13:02.12 SS.seek 25ml @16:13:39.09 delay 20 seconds @16:13:39.29 Stroke completed -- waiting for pressure to equalize ... @16:13:42.53 Intake.select :close @16:13:42.69 SSV.dial :exhaust @16:13:43.49 Exhaust.select :open @16:13:43.60 SS.reconfigure SSexhale @16:13:43.66 SS.seek 0ml @16:13:52.40 Exhaust.select :close @16:13:52.52 SSV.dial CBV @16:13:52.55 CBV.dial SSV @16:13:53.38 Allowing 130s for 25.0ml of remaining 1250.0ml @16:13:53.39 Intake.select :open @16:13:55.58 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:13:56.06 SS.reconfigure SSinhale.with maxSpeed: 48 @16:13:56.20 SS.seek 25ml @16:13:59.12 AS.seek 0.07ml @16:14:16.34 Vacuum too high -- waiting up to 40s @16:14:17.20 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:14:17.22 SS.reconfigure SSinhale.with maxSpeed: 43 @16:14:17.27 SS.seek 25ml @16:14:36.74 Stroke completed -- waiting for pressure to equalize ... @16:14:39.37 Intake.select :close @16:14:39.54 SSV.dial :exhaust @16:14:40.33 Exhaust.select :open @16:14:40.44 SS.reconfigure SSexhale @16:14:40.50 SS.seek 0ml @16:14:45.67 delay 20 seconds @16:14:49.68 Exhaust.select :close @16:14:49.80 SSV.dial CBV @16:14:49.83 CBV.dial SSV @16:14:50.66 Allowing 130s for 25.0ml of remaining 1225.0ml @16:14:50.69 Intake.select :open @16:14:52.87 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:14:52.89 SS.reconfigure SSinhale.with maxSpeed: 43 @16:14:52.94 SS.seek 25ml @16:15:05.69 ATV.dial :waste2 @16:15:06.40 AS.reconfigure ASconfig @16:15:06.45 AS.seek :empty @16:15:10.85 Elution @16:15:10.86 ATV.dial :air1 @16:15:11.93 AS.reconfigure ASslow1 @16:15:11.98 AS.seek 0.049ml @16:15:22.36 delay 3 seconds @16:15:25.38 ATV.dial :ElutionWater @16:15:26.04 AS.seek 0.109ml @16:15:33.79 Stroke completed -- waiting for pressure to equalize ... @16:15:36.41 Intake.select :close @16:15:36.57 SSV.dial :exhaust @16:15:37.36 Exhaust.select :open @16:15:37.48 SS.reconfigure SSexhale @16:15:37.54 SS.seek 0ml @16:15:38.37 delay 3 seconds @16:15:41.39 ATV.dial ASV,via: :air2 @16:15:43.33 ASV.dial :SPEelution @16:15:43.89 AFV.select :carrierSolution @16:15:44.05 AS.reconfigure ASnorm @16:15:44.10 AS.seek 0.759ml @16:15:46.42 Exhaust.select :close @16:15:46.53 SSV.dial CBV @16:15:46.56 CBV.dial SSV @16:15:47.38 Allowing 130s for 25.0ml of remaining 1200.0ml @16:15:47.39 Intake.select :open @16:15:49.58 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:15:49.60 SS.reconfigure SSinhale.with maxSpeed: 43 @16:15:49.65 SS.seek 25ml @16:16:17.20 delay 3 seconds @16:16:20.22 AFV.select :holdingCoil @16:16:20.38 AS.reconfigure ASslow1 @16:16:20.44 AS.seek 0.474ml @16:16:31.31 Stroke completed -- waiting for pressure to equalize ... @16:16:34.57 Intake.select :close @16:16:34.73 SSV.dial :exhaust @16:16:35.53 Exhaust.select :open @16:16:36.19 SS.reconfigure SSexhale @16:16:36.25 SS.seek 0ml @16:16:45.30 Exhaust.select :close @16:16:45.41 SSV.dial CBV @16:16:45.44 CBV.dial SSV @16:16:46.27 Allowing 130s for 25.0ml of remaining 1175.0ml @16:16:46.28 Intake.select :open @16:16:48.46 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:16:48.48 SS.reconfigure SSinhale.with maxSpeed: 43 @16:16:48.54 SS.seek 25ml @16:17:18.09 delay 5 seconds @16:17:23.19 AS.reconfigure ASslow2 @16:17:23.24 AS.seek 0.439ml @16:17:29.74 Stroke completed -- waiting for pressure to equalize ... @16:17:32.99 Intake.select :close @16:17:33.16 SSV.dial :exhaust @16:17:33.95 Exhaust.select :open @16:17:34.07 SS.reconfigure SSexhale @16:17:34.13 SS.seek 0ml @16:17:43.21 Exhaust.select :close @16:17:43.33 SSV.dial CBV @16:17:43.36 CBV.dial SSV @16:17:44.19 Allowing 130s for 25.0ml of remaining 1150.0ml @16:17:44.20 Intake.select :open @16:17:46.38 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:17:46.40 SS.reconfigure SSinhale.with maxSpeed: 43 @16:17:46.45 SS.seek 25ml @16:17:58.70 delay 1:00 @16:18:27.69 Stroke completed -- waiting for pressure to equalize ... @16:18:30.93 Intake.select :close @16:18:31.09 SSV.dial :exhaust @16:18:31.89 Exhaust.select :open @16:18:32.00 SS.reconfigure SSexhale @16:18:32.06 SS.seek 0ml @16:18:41.23 Exhaust.select :close @16:18:41.35 SSV.dial CBV @16:18:41.38 CBV.dial SSV @16:18:42.19 Allowing 130s for 25.0ml of remaining 1125.0ml @16:18:42.20 Intake.select :open @16:18:44.38 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:18:44.40 SS.reconfigure SSinhale.with maxSpeed: 43 @16:18:44.45 SS.seek 25ml @16:18:58.73 pushing eluate to holding coil @16:18:58.80 AS.seek 0.279ml @16:19:25.35 Stroke completed -- waiting for pressure to equalize ... @16:19:28.58 Intake.select :close @16:19:28.75 SSV.dial :exhaust @16:19:29.55 Exhaust.select :open @16:19:29.66 SS.reconfigure SSexhale @16:19:29.72 SS.seek 0ml @16:19:38.76 Exhaust.select :close @16:19:38.88 SSV.dial CBV @16:19:38.91 CBV.dial SSV @16:19:39.74 Allowing 130s for 25.0ml of remaining 1100.0ml @16:19:39.75 Intake.select :open @16:19:41.94 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:19:41.96 SS.reconfigure SSinhale.with maxSpeed: 43 @16:19:42.01 SS.seek 25ml @16:20:23.19 Stroke completed -- waiting for pressure to equalize ... @16:20:26.98 Intake.select :close @16:20:27.15 SSV.dial :exhaust @16:20:27.95 Exhaust.select :open @16:20:28.08 SS.reconfigure SSexhale @16:20:28.13 SS.seek 0ml @16:20:36.91 Exhaust.select :close @16:20:37.03 SSV.dial CBV @16:20:37.06 CBV.dial SSV @16:20:37.86 Allowing 130s for 25.0ml of remaining 1075.0ml @16:20:37.87 Intake.select :open @16:20:40.05 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:20:40.07 SS.reconfigure SSinhale.with maxSpeed: 43 @16:20:40.12 SS.seek 25ml @16:21:14.95 Vacuum too high -- waiting up to 40s @16:21:16.08 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:21:16.10 SS.reconfigure SSinhale.with maxSpeed: 39 @16:21:16.15 SS.seek 25ml @16:21:23.80 Stroke completed -- waiting for pressure to equalize ... @16:21:27.05 Intake.select :close @16:21:27.22 SSV.dial :exhaust @16:21:28.02 Exhaust.select :open @16:21:28.13 SS.reconfigure SSexhale @16:21:28.19 SS.seek 0ml @16:21:36.94 Exhaust.select :close @16:21:37.06 SSV.dial CBV @16:21:37.09 CBV.dial SSV @16:21:37.91 Allowing 130s for 25.0ml of remaining 1050.0ml @16:21:37.92 Intake.select :open @16:21:39.89 delay 30 seconds @16:21:40.10 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:21:40.12 SS.reconfigure SSinhale.with maxSpeed: 39 @16:21:40.17 SS.seek 25ml @16:22:09.92 ATV.dial :waste,via: :air3 @16:22:11.50 AS.reconfigure ASconfig @16:22:11.55 AS.seek :empty @16:22:25.90 Stroke completed -- waiting for pressure to equalize ... @16:22:27.93 Parking Bleach in LysateSource line and SPEcolumn @16:22:27.95 ATV.dial :air3 @16:22:28.73 AS.reconfigure ASslow1 @16:22:28.78 AS.seek 0.029ml @16:22:29.14 Intake.select :close @16:22:29.31 SSV.dial :exhaust @16:22:30.11 Exhaust.select :open @16:22:30.22 SS.reconfigure SSexhale @16:22:30.28 SS.seek 0ml @16:22:35.15 ATV.dial :ElutionWater @16:22:35.74 AS.seek 0.059ml @16:22:39.48 Exhaust.select :close @16:22:39.60 SSV.dial CBV @16:22:39.63 CBV.dial SSV @16:22:40.46 Allowing 130s for 25.0ml of remaining 1025.0ml @16:22:40.48 Intake.select :open @16:22:42.05 ATV.dial :air3 @16:22:42.70 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:22:42.74 SS.reconfigure SSinhale.with maxSpeed: 39 @16:22:43.39 SS.seek 25ml @16:22:43.42 AS.seek 0.069ml @16:22:45.79 delay 2 seconds @16:22:47.81 ATV.dial :bleach @16:22:48.27 AS.seek 0.769ml @16:23:28.88 Stroke completed -- waiting for pressure to equalize ... @16:23:32.78 Intake.select :close @16:23:32.94 SSV.dial :exhaust @16:23:33.74 Exhaust.select :open @16:23:33.85 SS.reconfigure SSexhale @16:23:33.92 SS.seek 0ml @16:23:42.69 Exhaust.select :close @16:23:42.81 SSV.dial CBV @16:23:42.84 CBV.dial SSV @16:23:43.66 Allowing 130s for 25.0ml of remaining 1000.0ml @16:23:43.67 Intake.select :open @16:23:45.86 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:23:45.88 SS.reconfigure SSinhale.with maxSpeed: 39 @16:23:45.93 SS.seek 25ml @16:24:31.41 Stroke completed -- waiting for pressure to equalize ... @16:24:35.30 Intake.select :close @16:24:35.46 SSV.dial :exhaust @16:24:36.25 Exhaust.select :open @16:24:36.36 SS.reconfigure SSexhale @16:24:36.42 SS.seek 0ml @16:24:45.91 Exhaust.select :close @16:24:46.03 SSV.dial CBV @16:24:46.06 CBV.dial SSV @16:24:46.89 Allowing 130s for 25.0ml of remaining 975.0ml @16:24:46.90 Intake.select :open @16:24:49.09 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:24:49.11 SS.reconfigure SSinhale.with maxSpeed: 39 @16:24:49.16 SS.seek 25ml @16:25:09.28 delay 3 seconds @16:25:12.30 ATV.dial :air3 @16:25:12.83 delay 3 seconds @16:25:15.92 AS.seek 0.794ml @16:25:21.19 delay 3 seconds @16:25:24.21 ATV.dial :bleach @16:25:24.80 AS.seek 0.779ml @16:25:28.11 delay 3 seconds @16:25:31.13 ATV.dial :AIV @16:25:32.30 AS.reconfigure ASconfig @16:25:32.35 AS.seek 0.015ml @16:25:34.26 Stroke completed -- waiting for pressure to equalize ... @16:25:38.77 Intake.select :close @16:25:38.93 SSV.dial :exhaust @16:25:39.73 Exhaust.select :open @16:25:39.85 SS.reconfigure SSexhale @16:25:39.91 SS.seek 0ml @16:25:48.73 Exhaust.select :close @16:25:48.85 SSV.dial CBV @16:25:48.88 CBV.dial SSV @16:25:49.70 Allowing 130s for 25.0ml of remaining 950.0ml @16:25:49.71 Intake.select :open @16:25:52.45 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:25:52.47 SS.reconfigure SSinhale.with maxSpeed: 39 @16:25:52.52 SS.seek 25ml @16:26:16.57 ATV.dial :waste @16:26:17.95 AS.seek :empty @16:26:19.16 ATV.dial :air3 @16:26:19.91 AS.reconfigure ASslow1 @16:26:19.96 AS.seek 0.009ml @16:26:22.28 delay 2 seconds @16:26:24.30 ATV.dial :bleach @16:26:24.76 AS.seek 0.579ml @16:26:29.45 Vacuum too high -- waiting up to 40s @16:26:30.84 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:26:30.86 SS.reconfigure SSinhale.with maxSpeed: 35 @16:26:30.91 SS.seek 25ml @16:26:40.54 Stroke completed -- waiting for pressure to equalize ... @16:26:44.42 Intake.select :close @16:26:44.59 SSV.dial :exhaust @16:26:45.39 Exhaust.select :open @16:26:45.50 SS.reconfigure SSexhale @16:26:45.57 SS.seek 0ml @16:26:54.38 Exhaust.select :close @16:26:54.50 SSV.dial CBV @16:26:54.53 CBV.dial SSV @16:26:55.34 Allowing 130s for 25.0ml of remaining 925.0ml @16:26:55.35 Intake.select :open @16:26:57.53 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:26:57.55 SS.reconfigure SSinhale.with maxSpeed: 35 @16:26:57.60 SS.seek 25ml @16:27:48.29 Stroke completed -- waiting for pressure to equalize ... @16:27:52.15 Intake.select :close @16:27:52.32 SSV.dial :exhaust @16:27:53.11 Exhaust.select :open @16:27:53.22 SS.reconfigure SSexhale @16:27:53.28 SS.seek 0ml @16:28:02.10 Exhaust.select :close @16:28:02.22 SSV.dial CBV @16:28:02.25 CBV.dial SSV @16:28:03.06 Allowing 130s for 25.0ml of remaining 900.0ml @16:28:03.07 Intake.select :open @16:28:05.26 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:28:05.28 SS.reconfigure SSinhale.with maxSpeed: 35 @16:28:05.33 SS.seek 25ml @16:28:19.66 delay 3 seconds @16:28:22.68 ATV.dial :air3 @16:28:23.22 delay 3 seconds @16:28:26.31 AS.seek 0.604ml @16:28:31.64 delay 3 seconds @16:28:34.66 ATV.dial :bleach @16:28:35.26 AS.seek 0.589ml @16:28:38.57 delay 3 seconds @16:28:41.60 ATV.dial :ASV @16:28:42.56 ASV.dial :wasteR @16:28:43.12 AS.seek 0ml @16:28:56.04 Stroke completed -- waiting for pressure to equalize ... @16:29:00.50 Intake.select :close @16:29:00.67 SSV.dial :exhaust @16:29:01.48 Exhaust.select :open @16:29:01.60 SS.reconfigure SSexhale @16:29:01.66 SS.seek 0ml @16:29:10.70 Exhaust.select :close @16:29:10.81 SSV.dial CBV @16:29:10.84 CBV.dial SSV @16:29:11.66 Allowing 130s for 25.0ml of remaining 875.0ml @16:29:11.67 Intake.select :open @16:29:13.85 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:29:13.87 SS.reconfigure SSinhale.with maxSpeed: 35 @16:29:13.92 SS.seek 25ml @16:30:04.27 Stroke completed -- waiting for pressure to equalize ... @16:30:08.82 Intake.select :close @16:30:08.98 SSV.dial :exhaust @16:30:09.79 Exhaust.select :open @16:30:09.91 SS.reconfigure SSexhale @16:30:09.97 SS.seek 0ml @16:30:19.10 Exhaust.select :close @16:30:19.22 SSV.dial CBV @16:30:19.25 CBV.dial SSV @16:30:20.08 Allowing 130s for 25.0ml of remaining 850.0ml @16:30:20.09 Intake.select :open @16:30:22.27 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:30:22.29 SS.reconfigure SSinhale.with maxSpeed: 35 @16:30:22.34 SS.seek 25ml @16:30:41.81 delay 1:00 @16:31:12.44 Stroke completed -- waiting for pressure to equalize ... @16:31:16.96 Intake.select :close @16:31:17.12 SSV.dial :exhaust @16:31:17.93 Exhaust.select :open @16:31:18.05 SS.reconfigure SSexhale @16:31:18.11 SS.seek 0ml @16:31:27.15 Exhaust.select :close @16:31:27.28 SSV.dial CBV @16:31:27.31 CBV.dial SSV @16:31:28.14 Allowing 130s for 25.0ml of remaining 825.0ml @16:31:28.15 Intake.select :open @16:31:30.33 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:31:30.35 SS.reconfigure SSinhale.with maxSpeed: 35 @16:31:30.40 SS.seek 25ml @16:31:41.83 AFV.select :carrierSolution @16:31:41.94 delay 2 seconds @16:31:43.97 AS.reconfigure ASconfig @16:31:44.02 AS.seek 1ml @16:32:20.65 Stroke completed -- waiting for pressure to equalize ... @16:32:25.73 Intake.select :close @16:32:25.90 SSV.dial :exhaust @16:32:26.70 Exhaust.select :open @16:32:26.82 SS.reconfigure SSexhale @16:32:26.88 SS.seek 0ml @16:32:36.04 Exhaust.select :close @16:32:36.16 SSV.dial CBV @16:32:36.19 CBV.dial SSV @16:32:37.02 Allowing 130s for 25.0ml of remaining 800.0ml @16:32:37.03 Intake.select :open @16:32:39.21 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:32:39.23 SS.reconfigure SSinhale.with maxSpeed: 35 @16:32:39.28 SS.seek 25ml @16:32:41.78 delay 1 second @16:32:42.79 AFV.select :holdingCoil @16:32:42.90 delay 1 second @16:32:43.92 ATV.dial ASV @16:32:43.94 ASV.dial :wasteR @16:32:43.96 AS.reconfigure ASslow1 @16:32:44.01 AS.seek 0ml @16:33:20.52 Vacuum too high -- waiting up to 40s @16:33:21.88 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:33:21.91 SS.reconfigure SSinhale.with maxSpeed: 32 @16:33:21.96 SS.seek 25ml @16:33:31.72 Stroke completed -- waiting for pressure to equalize ... @16:33:36.23 Intake.select :close @16:33:36.39 SSV.dial :exhaust @16:33:37.20 Exhaust.select :open @16:33:37.31 SS.reconfigure SSexhale @16:33:37.37 SS.seek 0ml @16:33:46.49 Exhaust.select :close @16:33:46.61 SSV.dial CBV @16:33:46.64 CBV.dial SSV @16:33:47.45 Allowing 130s for 25.0ml of remaining 775.0ml @16:33:47.46 Intake.select :open @16:33:49.65 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:33:49.67 SS.reconfigure SSinhale.with maxSpeed: 32 @16:33:49.72 SS.seek 25ml @16:34:44.68 Stroke completed -- waiting for pressure to equalize ... @16:34:49.82 Intake.select :close @16:34:49.99 SSV.dial :exhaust @16:34:50.78 Exhaust.select :open @16:34:50.90 SS.reconfigure SSexhale @16:34:50.96 SS.seek 0ml @16:34:59.80 Exhaust.select :close @16:34:59.92 SSV.dial CBV @16:34:59.95 CBV.dial SSV @16:35:00.78 Allowing 130s for 25.0ml of remaining 750.0ml @16:35:00.79 Intake.select :open @16:35:02.98 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:35:03.00 SS.reconfigure SSinhale.with maxSpeed: 32 @16:35:03.05 SS.seek 25ml @16:35:58.38 Stroke completed -- waiting for pressure to equalize ... @16:36:03.49 Intake.select :close @16:36:03.65 SSV.dial :exhaust @16:36:04.45 Exhaust.select :open @16:36:04.57 SS.reconfigure SSexhale @16:36:04.63 SS.seek 0ml @16:36:05.43 delay 20 seconds @16:36:13.69 Exhaust.select :close @16:36:13.80 SSV.dial CBV @16:36:13.83 CBV.dial SSV @16:36:14.66 Allowing 130s for 25.0ml of remaining 725.0ml @16:36:14.67 Intake.select :open @16:36:16.85 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:36:16.87 SS.reconfigure SSinhale.with maxSpeed: 32 @16:36:16.93 SS.seek 25ml @16:36:25.45 ATV.dial :air3 @16:36:26.87 AS.seek 0.59ml @16:37:11.76 Stroke completed -- waiting for pressure to equalize ... @16:37:17.58 Intake.select :close @16:37:17.75 SSV.dial :exhaust @16:37:18.55 Exhaust.select :open @16:37:18.66 SS.reconfigure SSexhale @16:37:18.72 SS.seek 0ml @16:37:28.07 Exhaust.select :close @16:37:28.19 SSV.dial CBV @16:37:28.22 CBV.dial SSV @16:37:29.05 Allowing 130s for 25.0ml of remaining 700.0ml @16:37:29.06 Intake.select :open @16:37:31.24 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:37:31.26 SS.reconfigure SSinhale.with maxSpeed: 32 @16:37:31.31 SS.seek 25ml @16:38:24.64 Vacuum too high -- waiting up to 40s @16:38:25.78 delay 3 seconds @16:38:26.28 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:38:26.31 SS.reconfigure SSinhale.with maxSpeed: 29 @16:38:26.35 SS.seek 25ml @16:38:28.41 Stroke completed -- waiting for pressure to equalize ... @16:38:28.80 ATV.dial :ASV @16:38:30.10 ASV.dial :wasteR @16:38:30.19 AS.seek 0.09ml @16:38:32.35 Intake.select :close @16:38:32.52 SSV.dial :exhaust @16:38:33.31 Exhaust.select :open @16:38:33.99 SS.reconfigure SSexhale @16:38:34.05 SS.seek 0ml @16:38:43.43 Exhaust.select :close @16:38:43.55 SSV.dial CBV @16:38:43.58 CBV.dial SSV @16:38:44.39 Allowing 130s for 25.0ml of remaining 675.0ml @16:38:44.40 Intake.select :open @16:38:46.58 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:38:46.61 SS.reconfigure SSinhale.with maxSpeed: 29 @16:38:46.66 SS.seek 25ml @16:39:46.74 Stroke completed -- waiting for pressure to equalize ... @16:39:51.35 Intake.select :close @16:39:51.51 SSV.dial :exhaust @16:39:52.31 Exhaust.select :open @16:39:52.43 SS.reconfigure SSexhale @16:39:52.49 SS.seek 0ml @16:40:01.30 Exhaust.select :close @16:40:01.41 SSV.dial CBV @16:40:01.44 CBV.dial SSV @16:40:02.27 Allowing 130s for 25.0ml of remaining 650.0ml @16:40:02.28 Intake.select :open @16:40:04.47 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:40:04.49 SS.reconfigure SSinhale.with maxSpeed: 29 @16:40:04.54 SS.seek 25ml @16:40:11.00 delay 30 seconds @16:40:41.02 ATV.dial :extSrc @16:40:41.75 delay 3 seconds @16:40:44.77 ATV.dial :ElutionWater @16:40:45.82 AS.seek 0.12ml @16:40:52.13 ATV.dial :air3 @16:40:52.73 AS.seek 0.13ml @16:40:55.00 delay 2 seconds @16:40:57.01 ATV.dial :bleach @16:40:57.48 AS.seek 0.23ml @16:41:05.39 Stroke completed -- waiting for pressure to equalize ... @16:41:10.58 Intake.select :close @16:41:10.74 SSV.dial :exhaust @16:41:11.53 Exhaust.select :open @16:41:11.64 SS.reconfigure SSexhale @16:41:11.70 SS.seek 0ml @16:41:17.86 delay 3 seconds @16:41:20.88 ATV.dial :air3 @16:41:21.01 Exhaust.select :close @16:41:21.13 SSV.dial CBV @16:41:21.16 CBV.dial SSV @16:41:21.47 delay 3 seconds @16:41:21.98 Allowing 130s for 25.0ml of remaining 625.0ml @16:41:21.99 Intake.select :open @16:41:24.18 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:41:24.20 SS.reconfigure SSinhale.with maxSpeed: 29 @16:41:24.25 SS.seek 25ml @16:41:24.60 AS.seek 0.255ml @16:41:29.92 delay 3 seconds @16:41:32.94 ATV.dial :bleach @16:41:33.54 AS.seek 0.24ml @16:41:36.85 delay 3 seconds @16:41:39.87 ATV.dial :ASV @16:41:40.83 ASV.dial :SPEdelivery @16:41:41.21 AFV.select :carrierSolution @16:41:41.32 delay 2 seconds @16:41:43.35 AS.reconfigure ASconfig @16:41:43.95 AS.seek 0.5ml @16:41:59.19 delay 1 second @16:42:00.20 AFV.select :holdingCoil @16:42:00.36 AS.reconfigure ASslow1 @16:42:00.42 AS.seek 0.17ml @16:42:24.86 Stroke completed -- waiting for pressure to equalize ... @16:42:30.03 Intake.select :close @16:42:30.20 SSV.dial :exhaust @16:42:31.00 Exhaust.select :open @16:42:31.11 SS.reconfigure SSexhale @16:42:31.17 SS.seek 0ml @16:42:40.28 Exhaust.select :close @16:42:40.39 SSV.dial CBV @16:42:40.42 CBV.dial SSV @16:42:41.25 Allowing 130s for 25.0ml of remaining 600.0ml @16:42:41.26 Intake.select :open @16:42:43.45 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:42:43.49 SS.reconfigure SSinhale.with maxSpeed: 29 @16:42:43.59 SS.seek 25ml @16:43:07.09 SPE.hold 2:46:40,95C @16:43:07.14 delay 3:00 @16:43:44.18 Stroke completed -- waiting for pressure to equalize ... @16:43:50.00 Intake.select :close @16:43:50.17 SSV.dial :exhaust @16:43:50.98 Exhaust.select :open @16:43:51.10 SS.reconfigure SSexhale @16:43:51.16 SS.seek 0ml @16:44:00.39 Exhaust.select :close @16:44:00.50 SSV.dial CBV @16:44:00.53 CBV.dial SSV @16:44:01.37 Allowing 130s for 25.0ml of remaining 575.0ml @16:44:01.38 Intake.select :open @16:44:03.56 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:44:03.58 SS.reconfigure SSinhale.with maxSpeed: 29 @16:44:03.64 SS.seek 25ml @16:44:48.76 Vacuum too high -- waiting up to 40s @16:44:50.41 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:44:50.43 SS.reconfigure SSinhale.with maxSpeed: 26 @16:44:50.48 SS.seek 25ml @16:45:07.68 Stroke completed -- waiting for pressure to equalize ... @16:45:13.42 Intake.select :close @16:45:13.59 SSV.dial :exhaust @16:45:14.42 Exhaust.select :open @16:45:14.53 SS.reconfigure SSexhale @16:45:14.59 SS.seek 0ml @16:45:23.97 Exhaust.select :close @16:45:24.08 SSV.dial CBV @16:45:24.11 CBV.dial SSV @16:45:24.95 Allowing 130s for 25.0ml of remaining 550.0ml @16:45:24.96 Intake.select :open @16:45:27.14 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:45:27.16 SS.reconfigure SSinhale.with maxSpeed: 26 @16:45:27.21 SS.seek 25ml @16:46:07.19 AFV.select :carrierSolution @16:46:07.30 delay 2 seconds @16:46:09.33 AS.reconfigure ASconfig @16:46:09.38 AS.seek 1ml @16:46:34.13 Stroke completed -- waiting for pressure to equalize ... @16:46:39.38 Intake.select :close @16:46:39.54 SSV.dial :exhaust @16:46:40.36 Exhaust.select :open @16:46:40.48 SS.reconfigure SSexhale @16:46:40.54 SS.seek 0ml @16:46:50.17 Exhaust.select :close @16:46:50.28 SSV.dial CBV @16:46:50.31 CBV.dial SSV @16:46:51.14 Allowing 130s for 25.0ml of remaining 525.0ml @16:46:51.15 Intake.select :open @16:46:53.33 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:46:53.35 SS.reconfigure SSinhale.with maxSpeed: 26 @16:46:53.40 SS.seek 25ml @16:46:57.41 delay 1 second @16:46:58.43 AFV.select :holdingCoil @16:46:58.54 delay 1 second @16:46:59.56 ASV.dial :wasteD @16:47:00.52 AS.seek 0ml @16:47:58.32 Priming Eluate @16:47:58.33 AFV.select :carrierSolution @16:47:58.44 delay 2 seconds @16:48:00.47 AS.seek 0.5ml @16:48:00.69 Stroke completed -- waiting for pressure to equalize ... @16:48:06.50 Intake.select :close @16:48:06.66 SSV.dial :exhaust @16:48:07.47 Exhaust.select :open @16:48:07.58 SS.reconfigure SSexhale @16:48:07.63 SS.seek 0ml @16:48:16.50 Exhaust.select :close @16:48:16.62 SSV.dial CBV @16:48:16.65 CBV.dial SSV @16:48:17.50 Allowing 130s for 25.0ml of remaining 500.0ml @16:48:17.51 Intake.select :open @16:48:19.70 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:48:19.72 SS.reconfigure SSinhale.with maxSpeed: 26 @16:48:19.77 SS.seek 25ml @16:48:29.51 delay 1 second @16:48:30.53 AFV.select :holdingCoil @16:48:30.64 ATV.dial ASV @16:48:30.65 ASV.dial :SPErecovery @16:48:31.21 Swish #1 of 2 @16:48:31.29 AS.reconfigure ASslow0 @16:48:31.34 AS.seek 0.6ml @16:48:42.37 AS.seek 0.5ml @16:48:52.71 Swish #2 of 2 @16:48:52.80 AS.seek 0.6ml @16:49:03.29 AS.seek 0.5ml @16:49:13.65 delay 3 seconds @16:49:16.73 AS.reconfigure ASslow1 @16:49:16.77 AS.seek 0.684ml @16:49:27.83 Stroke completed -- waiting for pressure to equalize ... @16:49:35.40 Intake.select :close @16:49:35.82 SSV.dial :exhaust @16:49:36.69 Exhaust.select :open @16:49:36.94 SS.reconfigure SSexhale @16:49:37.14 SS.seek 0ml @16:49:46.47 Exhaust.select :close @16:49:46.74 SSV.dial CBV @16:49:46.87 CBV.dial SSV @16:49:47.75 Allowing 130s for 25.0ml of remaining 475.0ml @16:49:47.76 Intake.select :open @16:49:49.95 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:49:49.97 SS.reconfigure SSinhale.with maxSpeed: 26 @16:49:50.02 SS.seek 25ml @16:49:54.07 delay 3 seconds @16:49:57.10 Eluate primed @16:49:57.11 ATV.dial :waste2 @16:49:57.68 AS.reconfigure ASnorm @16:49:57.73 AS.seek 0ml @16:50:32.53 SPE.coast @16:50:32.62 ASV.dial :SPErecovery @16:50:32.64 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0516h1500ml.pcr @16:50:32.66 Building PCR Slug from ASV with MM2 mix, PP4 primer @16:50:32.67 Priming with carrier @16:50:32.68 ATV.dial ARV @16:50:33.69 ARV.dial :carrier @16:50:34.03 AS.seek 0.5ml @16:50:50.01 Vacuum too high -- waiting up to 40s @16:50:51.65 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:50:51.67 SS.reconfigure SSinhale.with maxSpeed: 23 @16:50:51.72 SS.seek 25ml @16:50:59.59 AFV.select :carrierSolution @16:50:59.71 delay 2 seconds @16:51:00.43 Stroke completed -- waiting for pressure to equalize ... @16:51:01.74 AS.reconfigure ASconfig @16:51:01.79 AS.seek 1ml @16:51:06.21 Intake.select :close @16:51:06.37 SSV.dial :exhaust @16:51:07.17 Exhaust.select :open @16:51:07.28 SS.reconfigure SSexhale @16:51:07.33 SS.seek 0ml @16:51:16.72 Exhaust.select :close @16:51:16.84 SSV.dial CBV @16:51:16.87 CBV.dial SSV @16:51:17.69 Allowing 130s for 25.0ml of remaining 450.0ml @16:51:17.70 Intake.select :open @16:51:19.88 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:51:19.90 SS.reconfigure SSinhale.with maxSpeed: 23 @16:51:19.95 SS.seek 25ml @16:51:30.87 delay 1 second @16:51:32.44 AFV.select :holdingCoil @16:51:32.58 delay 1 second @16:51:33.60 ATV.dial :waste @16:51:34.99 AS.reconfigure ASnorm @16:51:35.04 AS.seek :empty @16:52:25.79 AFV.select :carrierSolution @16:52:25.90 delay 2 seconds @16:52:27.93 AS.reconfigure ASconfig @16:52:27.98 AS.seek 0.5ml @16:52:36.05 Stroke completed -- waiting for pressure to equalize ... @16:52:42.48 Intake.select :close @16:52:42.64 SSV.dial :exhaust @16:52:43.44 Exhaust.select :open @16:52:43.55 SS.reconfigure SSexhale @16:52:43.61 SS.seek 0ml @16:52:52.74 Exhaust.select :close @16:52:52.86 SSV.dial CBV @16:52:52.89 CBV.dial SSV @16:52:53.72 Allowing 130s for 25.0ml of remaining 425.0ml @16:52:53.73 Intake.select :open @16:52:55.92 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:52:55.94 SS.reconfigure SSinhale.with maxSpeed: 23 @16:52:56.03 SS.seek 25ml @16:52:57.12 delay 1 second @16:52:58.13 AFV.select :holdingCoil @16:52:58.24 Building Slug @16:52:58.25 ATV.dial :ARV @16:52:59.74 delay 2 seconds @16:53:01.76 ARV.dial :air1 @16:53:02.24 delay 2 seconds @16:53:04.33 AS.reconfigure ASslow2 @16:53:04.38 AS.seek 0.506ml @16:53:10.69 delay 2 seconds @16:53:12.71 ARV.dial :MM2 @16:53:14.10 delay 2 seconds @16:53:16.13 Adding MM2 @16:53:16.20 AS.seek 0.524ml @16:53:34.41 delay 25 seconds @16:53:59.43 ARV.dial :PP4 @16:53:59.82 Adding PP4 @16:53:59.83 delay 2 seconds @16:54:01.92 AS.seek 0.53ml @16:54:08.26 delay 10 seconds @16:54:11.94 Stroke completed -- waiting for pressure to equalize ... @16:54:18.34 ARV.dial :air3 @16:54:18.41 Intake.select :close @16:54:18.57 SSV.dial :exhaust @16:54:18.81 adding ARV air @16:54:18.83 delay 2 seconds @16:54:19.36 Exhaust.select :open @16:54:19.47 SS.reconfigure SSexhale @16:54:19.52 SS.seek 0ml @16:54:20.91 AS.seek 0.536ml @16:54:27.27 delay 5 seconds @16:54:28.86 Exhaust.select :close @16:54:28.98 SSV.dial CBV @16:54:29.01 CBV.dial SSV @16:54:30.44 Allowing 130s for 25.0ml of remaining 400.0ml @16:54:30.45 Intake.select :open @16:54:32.28 ARV.dial :carrier @16:54:32.64 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:54:32.66 SS.reconfigure SSinhale.with maxSpeed: 23 @16:54:32.71 SS.seek 25ml @16:54:33.48 delay 7 seconds @16:54:40.57 AS.seek 0.594ml @16:55:39.05 delay 5 seconds @16:55:44.07 ATV.dial :ASV @16:55:44.57 delay 2 seconds @16:55:46.66 AS.seek 0.6ml @16:55:48.36 Stroke completed -- waiting for pressure to equalize ... @16:55:52.90 delay 4 seconds @16:55:54.85 Intake.select :close @16:55:55.02 SSV.dial :exhaust @16:55:55.81 Exhaust.select :open @16:55:55.92 SS.reconfigure SSexhale @16:55:55.98 SS.seek 0ml @16:55:56.93 ATV.dial ARV @16:55:57.45 delay 2 seconds @16:55:59.47 ARV.dial :carrier @16:55:59.49 delay 2 seconds @16:56:01.58 AS.seek 0.65ml @16:56:04.97 Exhaust.select :close @16:56:05.09 SSV.dial CBV @16:56:05.12 CBV.dial SSV @16:56:05.94 Allowing 130s for 25.0ml of remaining 375.0ml @16:56:05.95 Intake.select :open @16:56:08.14 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:56:08.16 SS.reconfigure SSinhale.with maxSpeed: 23 @16:56:08.21 SS.seek 25ml @16:56:51.99 delay 4 seconds @16:56:56.01 ATV.dial PCR @16:56:56.59 ASV.dial :waste1 @16:56:57.35 ARV.dial :air1 @16:56:57.73 Positioning Slug by 0.324ml @16:56:57.82 AS.seek 0.326ml @16:57:24.65 Stroke completed -- waiting for pressure to equalize ... @16:57:31.71 Intake.select :close @16:57:31.88 SSV.dial :exhaust @16:57:32.67 Exhaust.select :open @16:57:32.78 SS.reconfigure SSexhale @16:57:32.83 SS.seek 0ml @16:57:42.07 Exhaust.select :close @16:57:42.19 SSV.dial CBV @16:57:42.22 CBV.dial SSV @16:57:43.05 Allowing 130s for 25.0ml of remaining 350.0ml @16:57:43.06 Intake.select :open @16:57:45.84 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:57:45.86 SS.reconfigure SSinhale.with maxSpeed: 23 @16:57:45.91 SS.seek 25ml @16:58:54.71 Vacuum too high -- waiting up to 40s @16:58:56.61 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:58:56.63 SS.reconfigure SSinhale.with maxSpeed: 21 @16:58:56.69 SS.seek 25ml @16:59:05.08 Stroke completed -- waiting for pressure to equalize ... @16:59:11.52 Intake.select :close @16:59:11.68 SSV.dial :exhaust @16:59:12.48 Exhaust.select :open @16:59:12.59 SS.reconfigure SSexhale @16:59:12.65 SS.seek 0ml @16:59:21.77 Exhaust.select :close @16:59:21.89 SSV.dial CBV @16:59:21.92 CBV.dial SSV @16:59:22.73 Allowing 130s for 25.0ml of remaining 325.0ml @16:59:22.74 Intake.select :open @16:59:24.93 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:59:24.95 SS.reconfigure SSinhale.with maxSpeed: 21 @16:59:25.00 SS.seek 25ml @17:00:48.29 Stroke completed -- waiting for pressure to equalize ... @17:00:55.98 Intake.select :close @17:00:56.14 SSV.dial :exhaust @17:00:56.94 Exhaust.select :open @17:00:57.05 SS.reconfigure SSexhale @17:00:57.10 SS.seek 0ml @17:01:05.94 Exhaust.select :close @17:01:06.06 SSV.dial CBV @17:01:06.09 CBV.dial SSV @17:01:06.90 Allowing 130s for 25.0ml of remaining 300.0ml @17:01:06.91 Intake.select :open @17:01:09.09 Intake @34.9psia, vacuum from 20.0 to 24.0psi @17:01:09.11 SS.reconfigure SSinhale.with maxSpeed: 21 @17:01:09.16 SS.seek 25ml @17:02:24.04 delay 5 seconds @17:02:29.06 ATV.dial ARV @17:02:29.90 ASV.dial :PCRlock @17:02:30.26 delay 5 seconds @17:02:32.83 Stroke completed -- waiting for pressure to equalize ... @17:02:35.35 Running Tt1 Profile60C300 thermocycling @17:02:35.37 PCR.program Profile60C300 @17:02:35.51 Profile60C300 should complete in < 1:45:21 @17:02:35.52 PCR.run 2:16:41.25 @17:02:40.71 Intake.select :close @17:02:40.87 SSV.dial :exhaust @17:02:41.67 Exhaust.select :open @17:02:41.79 SS.reconfigure SSexhale @17:02:41.85 SS.seek 0ml @17:02:51.00 Exhaust.select :close @17:02:51.12 SSV.dial CBV @17:02:51.15 CBV.dial SSV @17:02:51.97 Allowing 130s for 25.0ml of remaining 275.0ml @17:02:51.98 Intake.select :open @17:02:54.17 Intake @35.0psia, vacuum from 20.0 to 24.0psi @17:02:54.19 SS.reconfigure SSinhale.with maxSpeed: 21 @17:02:54.24 SS.seek 25ml @17:04:02.92 Vacuum too high -- waiting up to 40s @17:04:04.80 Intake @34.7psia, vacuum from 20.0 to 24.0psi @17:04:04.82 SS.reconfigure SSinhale.with maxSpeed: 19 @17:04:04.87 SS.seek 25ml @17:04:21.13 Stroke completed -- waiting for pressure to equalize ... @17:04:28.86 Intake.select :close @17:04:29.02 SSV.dial :exhaust @17:04:29.81 Exhaust.select :open @17:04:29.92 SS.reconfigure SSexhale @17:04:29.97 SS.seek 0ml @17:04:39.38 Exhaust.select :close @17:04:39.50 SSV.dial CBV @17:04:39.53 CBV.dial SSV @17:04:40.55 Allowing 130s for 25.0ml of remaining 250.0ml @17:04:40.58 Intake.select :open @17:04:42.76 Intake @34.8psia, vacuum from 20.0 to 24.0psi @17:04:42.79 SS.reconfigure SSinhale.with maxSpeed: 19 @17:04:42.84 SS.seek 25ml @17:06:14.96 Stroke completed -- waiting for pressure to equalize ... @17:06:22.67 Intake.select :close @17:06:22.83 SSV.dial :exhaust @17:06:23.62 Exhaust.select :open @17:06:23.75 SS.reconfigure SSexhale @17:06:23.80 SS.seek 0ml @17:06:32.99 Exhaust.select :close @17:06:33.11 SSV.dial CBV @17:06:33.14 CBV.dial SSV @17:06:33.97 Allowing 130s for 25.0ml of remaining 225.0ml @17:06:33.98 Intake.select :open @17:06:36.17 Intake @34.6psia, vacuum from 20.0 to 24.0psi @17:06:36.19 SS.reconfigure SSinhale.with maxSpeed: 19 @17:06:36.24 SS.seek 25ml @17:08:08.30 Stroke completed -- waiting for pressure to equalize ... @17:08:16.65 Intake.select :close @17:08:16.82 SSV.dial :exhaust @17:08:17.61 Exhaust.select :open @17:08:17.72 SS.reconfigure SSexhale @17:08:17.77 SS.seek 0ml @17:08:27.03 Exhaust.select :close @17:08:27.15 SSV.dial CBV @17:08:27.18 CBV.dial SSV @17:08:27.98 Allowing 130s for 25.0ml of remaining 200.0ml @17:08:27.99 Intake.select :open @17:08:30.17 Intake @34.9psia, vacuum from 20.0 to 24.0psi @17:08:30.71 SS.reconfigure SSinhale.with maxSpeed: 19 @17:08:30.85 SS.seek 25ml @17:10:02.71 Stroke completed -- waiting for pressure to equalize ... @17:10:11.73 Intake.select :close @17:10:11.89 SSV.dial :exhaust @17:10:12.69 Exhaust.select :open @17:10:12.81 SS.reconfigure SSexhale @17:10:12.88 SS.seek 0ml @17:10:22.33 Exhaust.select :close @17:10:22.45 SSV.dial CBV @17:10:22.48 CBV.dial SSV @17:10:23.31 Allowing 130s for 25.0ml of remaining 175.0ml @17:10:23.32 Intake.select :open @17:10:25.51 Intake @34.9psia, vacuum from 20.0 to 24.0psi @17:10:25.53 SS.reconfigure SSinhale.with maxSpeed: 19 @17:10:25.58 SS.seek 25ml @17:11:44.51 Vacuum too high -- waiting up to 40s @17:11:46.67 Intake @34.9psia, vacuum from 20.0 to 24.0psi @17:11:46.69 SS.reconfigure SSinhale.with maxSpeed: 17 @17:11:46.74 SS.seek 25ml @17:12:01.74 Stroke completed -- waiting for pressure to equalize ... @17:12:10.05 Intake.select :close @17:12:10.21 SSV.dial :exhaust @17:12:11.01 Exhaust.select :open @17:12:11.12 SS.reconfigure SSexhale @17:12:11.18 SS.seek 0ml @17:12:20.53 Exhaust.select :close @17:12:20.65 SSV.dial CBV @17:12:20.68 CBV.dial SSV @17:12:21.50 Allowing 130s for 25.0ml of remaining 150.0ml @17:12:21.51 Intake.select :open @17:12:23.70 Intake @34.9psia, vacuum from 20.0 to 24.0psi @17:12:23.72 SS.reconfigure SSinhale.with maxSpeed: 17 @17:12:23.77 SS.seek 25ml @17:14:06.58 Stroke completed -- waiting for pressure to equalize ... @17:14:16.19 Intake.select :close @17:14:16.35 SSV.dial :exhaust @17:14:17.14 Exhaust.select :open @17:14:17.25 SS.reconfigure SSexhale @17:14:17.30 SS.seek 0ml @17:14:26.13 Exhaust.select :close @17:14:26.25 SSV.dial CBV @17:14:26.28 CBV.dial SSV @17:14:27.11 Allowing 130s for 25.0ml of remaining 125.0ml @17:14:27.12 Intake.select :open @17:14:29.30 Intake @35.1psia, vacuum from 20.0 to 24.0psi @17:14:29.32 SS.reconfigure SSinhale.with maxSpeed: 17 @17:14:29.37 SS.seek 25ml @17:16:12.55 Stroke completed -- waiting for pressure to equalize ... @17:16:22.16 Intake.select :close @17:16:22.33 SSV.dial :exhaust @17:16:23.12 Exhaust.select :open @17:16:23.23 SS.reconfigure SSexhale @17:16:23.28 SS.seek 0ml @17:16:32.21 Exhaust.select :close @17:16:32.33 SSV.dial CBV @17:16:32.36 CBV.dial SSV @17:16:33.17 Allowing 130s for 25.0ml of remaining 100.0ml @17:16:33.18 Intake.select :open @17:16:35.37 Intake @34.9psia, vacuum from 20.0 to 24.0psi @17:16:35.39 SS.reconfigure SSinhale.with maxSpeed: 17 @17:16:35.44 SS.seek 25ml @17:18:18.74 Stroke completed -- waiting for pressure to equalize ... @17:18:29.02 Intake.select :close @17:18:29.19 SSV.dial :exhaust @17:18:29.98 Exhaust.select :open @17:18:30.09 SS.reconfigure SSexhale @17:18:30.14 SS.seek 0ml @17:18:39.54 Exhaust.select :close @17:18:39.66 SSV.dial CBV @17:18:39.69 CBV.dial SSV @17:18:40.51 Allowing 130s for 25.0ml of remaining 75.0ml @17:18:40.52 Intake.select :open @17:18:42.71 Intake @34.9psia, vacuum from 20.0 to 24.0psi @17:18:42.73 SS.reconfigure SSinhale.with maxSpeed: 17 @17:18:42.78 SS.seek 25ml @17:20:03.07 Vacuum too high -- waiting up to 40s @17:20:05.58 Intake @34.7psia, vacuum from 20.0 to 24.0psi @17:20:05.60 SS.reconfigure SSinhale.with maxSpeed: 15 @17:20:05.65 SS.seek 25ml @17:20:31.22 Stroke completed -- waiting for pressure to equalize ... @17:20:40.84 Intake.select :close @17:20:41.01 SSV.dial :exhaust @17:20:41.82 Exhaust.select :open @17:20:41.93 SS.reconfigure SSexhale @17:20:41.98 SS.seek 0ml @17:20:51.25 Exhaust.select :close @17:20:51.37 SSV.dial CBV @17:20:51.40 CBV.dial SSV @17:20:52.22 Allowing 130s for 25.0ml of remaining 50.0ml @17:20:52.23 Intake.select :open @17:20:54.41 Intake @34.8psia, vacuum from 20.0 to 24.0psi @17:20:54.43 SS.reconfigure SSinhale.with maxSpeed: 15 @17:20:54.48 SS.seek 25ml @17:22:50.80 Stroke completed -- waiting for pressure to equalize ... @17:23:01.06 Intake.select :close @17:23:01.22 SSV.dial :exhaust @17:23:02.03 Exhaust.select :open @17:23:02.15 SS.reconfigure SSexhale @17:23:02.20 SS.seek 0ml @17:23:11.30 Exhaust.select :close @17:23:11.42 SSV.dial CBV @17:23:11.45 CBV.dial SSV @17:23:12.28 Allowing 130s for 25.0ml of remaining 25.0ml @17:23:12.29 Intake.select :open @17:23:14.48 Intake @35.0psia, vacuum from 20.0 to 24.0psi @17:23:14.50 SS.reconfigure SSinhale.with maxSpeed: 15 @17:23:14.55 SS.seek 24.96ml @17:25:11.18 Stroke completed -- waiting for pressure to equalize ... @17:25:22.07 Intake.select :close @17:25:22.24 SSV.dial :exhaust @17:25:23.03 Exhaust.select :open @17:25:23.14 SS.reconfigure SSexhale @17:25:23.20 SS.seek 0ml @17:25:32.39 Exhaust.select :close @17:25:32.51 Sampled 1500.0ml @17:25:32.53 Drying puck before fixing for 4:00 @17:25:32.54 CBV.dial :waste2b @17:25:33.32 delay 1:00 @17:26:33.34 CSV.dial :air @17:26:33.82 CS.reconfigure CSfast @17:26:33.88 CS.seek 10ml @17:26:57.34 Pumping wcr puck from tube 2 up to 55psi... @17:26:57.36 CBV.dial SSV @17:26:58.69 CBV.dial between CSV and waste2b @17:26:59.70 CSV.dial between air and CTV @17:27:00.05 CS.seek 6.87ml @17:27:08.12 CTV.dial CSV,avoiding: :air @17:27:08.15 CSV.dial CTV @17:27:08.96 CS.reconfigure CSpressurize @17:27:09.02 CS.seek 0ml @17:27:24.73 CBV.dial :waste2b @17:27:25.09 CS.reconfigure CSconfig @17:27:25.14 CS.seek :empty @17:27:28.56 delay 1:00 @17:28:28.59 CTV.dial :air,avoiding: :intake @17:28:29.92 Fix #1 of 2 (with rnal) for 2:00 @17:28:29.94 CSV.dial CSR @17:28:30.46 Pulling 2ml of CSR.rnal @17:28:30.48 CSR.select :rnal @17:28:30.60 CS.seek 2ml @17:28:36.68 delay 5 seconds @17:28:41.70 CSR.select :air @17:28:41.83 CS.reconfigure CSfast @17:28:41.88 CS.seek 10ml @17:29:00.78 Pumping wcr puck from tube 2 up to 55psi... @17:29:00.79 CBV.dial SSV @17:29:02.14 CBV.dial between CSV and waste2b @17:29:03.16 CSV.dial between air and CTV @17:29:03.80 CS.seek 6.835ml @17:29:12.00 CTV.dial CSV,avoiding: :air @17:29:12.03 CSV.dial CTV @17:29:13.43 CS.reconfigure CSpressurize @17:29:13.49 CS.seek 0ml @17:29:30.64 delay 2:00 @17:31:30.66 CBV.dial :waste2b @17:31:31.01 CS.reconfigure CSconfig @17:31:31.06 CS.seek :empty @17:31:32.84 delay 1:00 @17:32:32.86 CTV.dial :air,avoiding: :intake @17:32:34.20 Fix #2 of 2 (with rnal) for 2:00 @17:32:34.22 CSV.dial CSR @17:32:34.73 Pulling 2ml of CSR.rnal @17:32:34.74 CSR.select :rnal @17:32:34.87 CS.seek 2ml @17:32:41.06 delay 5 seconds @17:32:46.08 CSR.select :air @17:32:46.21 CS.reconfigure CSfast @17:32:46.26 CS.seek 10ml @17:33:05.19 Pumping wcr puck from tube 2 up to 55psi... @17:33:05.20 CBV.dial SSV @17:33:06.53 CBV.dial between CSV and waste2b @17:33:07.54 CSV.dial between air and CTV @17:33:08.77 CS.seek 6.8ml @17:33:17.09 CTV.dial CSV,avoiding: :air @17:33:17.12 CSV.dial CTV @17:33:18.56 CS.reconfigure CSpressurize @17:33:18.61 CS.seek 0ml @17:33:35.11 delay 2:00 @17:35:35.13 CBV.dial :waste2b @17:35:35.48 CS.reconfigure CSconfig @17:35:35.53 CS.seek :empty @17:35:37.91 delay 1:00 @17:36:37.94 CTV.dial :air,avoiding: :intake @17:36:39.35 Storing wcr puck in Collection Clamp to tube 1 @17:36:39.55 SC.reconfigure SCtight @17:36:39.60 SC.seek 1 @17:36:41.77 SC.reconfigure SCloose @17:36:42.05 Elbow.reconfigure TightElbow @17:36:42.10 Elbow.seek 1 @17:36:44.02 Forearm.reconfigure TightForearm @17:36:44.08 Forearm.seek 1 + 700 counts @17:36:45.22 SE.reconfigure SEpick @17:36:45.29 SE.seek beamBreak @17:36:46.80 SE.configure SEconfig @17:36:46.97 Elbow.reconfigure LimpElbow @17:36:47.03 Forearm.reconfigure LooseForearm @17:36:47.08 Forearm.seek 1 @17:36:47.37 Elbow.hold @17:36:47.45 Elbow.reconfigure TightElbow @17:36:47.56 SE.seek 16.09 Pucks below Top Plate @17:36:48.09 Forearm.force -20 @17:36:48.14 Forearm.seek 1 + 347 counts @17:36:48.46 Forearm.reconfigure TightForearm @17:36:48.52 Forearm.seek 1 + 700 counts @17:36:48.85 SE.seek 17.09 Pucks below Top Plate @17:36:49.58 Forearm.reconfigure LooseForearm @17:36:49.63 Forearm.seek :retract @17:36:50.54 Elbow.seek CC @17:36:52.80 Elbow.reconfigure LooseElbow @17:36:52.95 Forearm.seek CC @17:36:53.93 Hand.close @17:36:54.51 CC.seek :open @17:36:59.06 Forearm.seek :retract @17:37:00.04 Elbow.reconfigure TightElbow @17:37:00.09 Elbow.seek 1 @17:37:01.95 Elbow.reconfigure LimpElbow @17:37:02.01 Forearm.seek 1 @17:37:03.01 Elbow.hold @17:37:03.09 Elbow.reconfigure TightElbow @17:37:03.14 Hand.open @17:37:03.90 Forearm.force -20 @17:37:03.96 Forearm.seek 1 + 347 counts @17:37:04.31 Forearm.reconfigure TightForearm @17:37:04.37 Forearm.seek 1 + 700 counts @17:37:04.78 SE.seek :down @17:37:05.95 Forearm.reconfigure LooseForearm @17:37:06.00 Forearm.seek :retract @17:37:06.07 SE.coast @17:37:06.92 SC.coast @17:37:06.96 Starting finalPurge @17:37:06.98 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:37:07.06 Hand.open @17:37:07.40 Elbow.seek FlushPuck::Garage @17:37:09.51 Elbow.reconfigure LooseElbow @17:37:09.61 Forearm.seek :garage @17:37:10.60 Hand.close @17:37:11.39 Forearm.seek :retract @17:37:12.42 Elbow.reconfigure TightElbow @17:37:12.47 Elbow.seek CC @17:37:15.40 Elbow.reconfigure LooseElbow @17:37:15.52 Forearm.reconfigure TightForearm @17:37:15.57 Forearm.seek CC @17:37:16.82 CC.seek :closed @17:37:21.08 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 1.87 @17:37:21.78 Hand.open @17:37:22.46 Forearm.reconfigure LooseForearm @17:37:22.51 Forearm.seek :retract @17:37:23.50 Flushing the Collection Valve::Manifold @17:37:23.64 CSV.dial CSR @17:37:24.16 Pulling 5ml of CSR.flush @17:37:24.17 CSR.select :flush @17:37:24.30 CS.seek 5ml @17:37:38.13 delay 5 seconds @17:37:43.14 CSR.select :air @17:37:43.33 CS.reconfigure CSfast @17:37:43.37 CS.seek 7.5ml @17:37:50.12 CSV.dial :waste @17:37:51.46 CS.reconfigure CSconfig @17:37:51.51 CS.seek 0ml @17:38:11.72 Flushing lower manifold's waste1 port @17:38:11.86 CSV.dial :flush @17:38:12.33 CS.reconfigure CSfast @17:38:12.38 CS.seek 5ml @17:38:24.60 delay 5 seconds @17:38:29.61 CSV.dial :air @17:38:30.17 CS.seek 7ml @17:38:35.82 CSV.dial CTV @17:38:35.85
CTV.dial CSV @17:38:37.25 CBV.dial :waste1 @17:38:38.04 CS.seek 0ml @17:38:54.69 Flushing lower manifold's waste2b port @17:38:54.83 CSV.dial :flush @17:38:55.61 CS.seek 5ml @17:39:07.88 delay 5 seconds @17:39:12.90 CSV.dial :air @17:39:13.43 CS.seek 7.001ml @17:39:18.94 CSV.dial CTV @17:39:18.97
CTV.dial CSV @17:39:19.44 CBV.dial :waste2b @17:39:20.24 CS.seek 0ml @17:39:36.94 Flushing upper manifold @17:39:37.08 CSV.dial :flush @17:39:37.86 CS.seek 5ml @17:39:50.25 delay 5 seconds @17:39:55.26 CSV.dial :air @17:39:55.81 CS.seek 7ml @17:40:01.32 CSV.dial CBV @17:40:01.39
CBV.dial CSV @17:40:02.52 CTV.dial :air @17:40:03.87 CS.seek 0ml @17:40:20.47 delay 7 seconds @17:40:27.49 CS.seek 3ml @17:40:35.21 CSV.dial :waste @17:40:35.69 CS.seek 0ml @17:40:43.56 CSV.dial :flush @17:40:44.05 CS.seek 3ml @17:40:52.44 delay 5 seconds @17:40:57.47 CSV.dial :air @17:40:58.01 CS.seek 5ml @17:41:03.63 CSV.dial :air @17:41:03.65 CS.seek 0ml @17:41:15.89 delay 7 seconds @17:41:22.92 CS.seek 3ml @17:41:30.88 CSV.dial :waste @17:41:31.64 CS.seek 0ml @17:41:39.32 Disinfecting external Intake with mfbkill @17:41:39.46 CSV.dial CSR @17:41:40.85 Pulling 8ml of CSR.mfbkill @17:41:40.86 CSR.select :mfbkill @17:41:40.99 CS.seek 8ml @17:41:59.82 delay 5 seconds @17:42:04.83 CSR.select :air @17:42:04.97 CS.seek 10ml @17:42:10.51 CSV.dial CBV @17:42:10.54
CBV.dial CSV @17:42:11.58 CTV.dial :intake @17:42:12.33 Intake.select :open @17:42:12.46 CS.seek 2ml @17:42:31.87 Intake.select :close @17:42:31.99 CTV.dial :air @17:42:32.76 CS.reconfigure CSconfig @17:42:32.81 CS.seek 0ml @17:42:38.88 Flushing lower manifold's waste1 port @17:42:38.89 CSV.dial CSR @17:42:39.99 Pulling 4ml of CSR.mfbkill @17:42:40.00 CSR.select :mfbkill @17:42:40.13 CS.seek 4ml @17:42:51.41 delay 5 seconds @17:42:56.42 CSR.select :air @17:42:56.55 CS.reconfigure CSfast @17:42:56.60 CS.seek 10ml @17:43:11.04 CSV.dial CTV @17:43:11.07
CTV.dial CSV @17:43:12.49 CBV.dial :waste1 @17:43:12.99 CS.seek 0ml @17:43:36.32 Flushing lower manifold's waste2b port @17:43:36.33 CSV.dial CSR @17:43:36.86 Pulling 4ml of CSR.mfbkill @17:43:36.87 CSR.select :mfbkill @17:43:37.00 CS.reconfigure CSconfig @17:43:37.04 CS.seek 4ml @17:43:48.38 delay 5 seconds @17:43:53.40 CSR.select :air @17:43:53.53 CS.reconfigure CSfast @17:43:53.58 CS.seek 10ml @17:44:07.91 CSV.dial CTV @17:44:07.94
CTV.dial CSV @17:44:08.43 CBV.dial :waste2b @17:44:09.24 CS.seek 0ml @17:44:32.38 Killing upper manifold @17:44:32.39 CSV.dial CSR @17:44:32.91 Pulling 4ml of CSR.mfbkill @17:44:32.92 CSR.select :mfbkill @17:44:33.05 CS.reconfigure CSconfig @17:44:33.09 CS.seek 4ml @17:44:44.35 delay 5 seconds @17:44:49.36 CSR.select :air @17:44:49.49 CS.reconfigure CSfast @17:44:49.54 CS.seek 10ml @17:45:04.38 CSV.dial CBV @17:45:04.41
CBV.dial CSV @17:45:05.49 CTV.dial :air @17:45:06.83 CS.seek 0ml @17:45:30.20 delay 7 seconds @17:45:37.22 CS.seek 3ml @17:45:45.03 CSV.dial :waste @17:45:45.52 CS.seek 0ml @17:45:53.20 Flushing upper manifold @17:45:53.34 CSV.dial :flush @17:45:53.82 CS.seek 5ml @17:46:06.01 delay 5 seconds @17:46:11.04 CSV.dial :air @17:46:11.69 CS.seek 7ml @17:46:17.20 CSV.dial CBV @17:46:17.23
CBV.dial CSV @17:46:18.27 CTV.dial :air @17:46:18.29 CS.seek 0ml @17:46:34.82 delay 7 seconds @17:46:41.85 CS.seek 3ml @17:46:49.69 CSV.dial :waste @17:46:50.19 CS.seek 0ml @17:46:58.05 CSV.dial :flush @17:46:58.52 CS.seek 3ml @17:47:06.32 delay 5 seconds @17:47:11.34 CSV.dial :air @17:47:11.86 CS.seek 5ml @17:47:17.44 CSV.dial :air @17:47:17.47 CS.seek 0ml @17:47:29.54 delay 7 seconds @17:47:36.56 CS.seek 3ml @17:47:44.34 CSV.dial :waste @17:47:45.11 CS.seek 0ml @17:47:52.82 Flushing lower manifold's waste1 port @17:47:52.96 CSV.dial :flush @17:47:53.44 CS.seek 5ml @17:48:05.60 delay 5 seconds @17:48:10.61 CSV.dial :air @17:48:11.14 CS.seek 7ml @17:48:16.73 CSV.dial CTV @17:48:16.76
CTV.dial CSV @17:48:18.17 CBV.dial :waste1 @17:48:18.68 CS.seek 0ml @17:48:35.26 Flushing lower manifold's waste2b port @17:48:35.40 CSV.dial :flush @17:48:36.18 CS.seek 5ml @17:48:48.32 delay 5 seconds @17:48:53.34 CSV.dial :air @17:48:53.89 CS.seek 7ml @17:48:59.42 CSV.dial CTV @17:48:59.45
CTV.dial CSV @17:48:59.90 CBV.dial :waste2b @17:49:01.30 CS.seek 0ml @17:49:17.86 Disinfecting external Intake with kill @17:49:18.00 CSV.dial CSR @17:49:18.53 Pulling 8ml of CSR.kill CSR.select :kill @17:49:18.66 CS.seek 8ml @17:49:37.36 delay 5 seconds @17:49:42.38 CSR.select :air @17:49:42.52 CS.seek 10ml @17:49:48.14 CSV.dial CBV @17:49:48.16
CBV.dial CSV @17:49:49.26 CTV.dial :intake @17:49:50.03 Intake.select :open @17:49:50.15 CS.seek 2ml @17:50:09.94 Intake.select :close @17:50:10.06 CTV.dial :air @17:50:10.83 CS.reconfigure CSconfig @17:50:10.88 CS.seek 0ml @17:50:16.94 CSV.dial CSR @17:50:18.04 Pulling 5ml of CSR.flush @17:50:18.05 CSR.select :flush @17:50:18.18 CS.seek 5ml @17:50:31.95 delay 5 seconds @17:50:36.97 CSR.select :air @17:50:37.10 CS.seek 10ml @17:50:50.83 CSV.dial CBV @17:50:51.88 CS.seek 0ml @17:51:18.45 Garaging FlushPuck in Collection Clamp @17:51:18.53 Elbow.reconfigure TightElbow @17:51:18.58 Elbow.seek CC @17:51:18.85 Elbow.reconfigure LooseElbow @17:51:18.95 Forearm.seek CC @17:51:19.91 Hand.close @17:51:20.53 CC.seek :open @17:51:24.86 Forearm.seek :retract @17:51:25.87 Elbow.reconfigure TightElbow @17:51:25.92 Elbow.seek :garage @17:51:29.01 Elbow.reconfigure LooseElbow @17:51:29.11 Forearm.reconfigure TightForearm @17:51:29.16 Forearm.seek :garage @17:51:30.30 Hand.open @17:51:30.98 Forearm.reconfigure LooseForearm @17:51:31.03 Forearm.seek :retract @17:51:32.06 Starting initialPurge @17:51:32.07 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:51:32.15 Hand.open @17:51:32.48 Elbow.reconfigure TightElbow @17:51:32.53 Elbow.seek FlushPuck::Garage @17:51:32.67 Elbow.reconfigure LooseElbow @17:51:32.78 Forearm.seek :garage @17:51:33.80 Hand.close @17:51:34.55 Forearm.seek :retract @17:51:35.56 Elbow.reconfigure TightElbow @17:51:35.61 Elbow.seek CC @17:51:38.53 Elbow.reconfigure LooseElbow @17:51:39.23 Forearm.reconfigure TightForearm @17:51:39.28 Forearm.seek CC @17:51:40.66 CC.seek :closed @17:51:44.90 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.88 @17:51:45.00 Hand.open @17:51:45.74 Forearm.reconfigure LooseForearm @17:51:45.78 Forearm.seek :retract @17:51:46.77 Flushing the Collection Valve::Manifold @17:51:46.91 CSV.dial CSR @17:51:48.01 Pulling 5ml of CSR.flush @17:51:48.02 CSR.select :flush @17:51:48.15 CS.seek 5ml @17:52:01.98 delay 5 seconds @17:52:06.99 CSR.select :air @17:52:07.16 CS.reconfigure CSfast @17:52:07.21 CS.seek 7.5ml @17:52:13.79 CSV.dial :waste @17:52:15.12 CS.reconfigure CSconfig @17:52:15.17 CS.seek 0ml @17:52:35.39 Purging external Intake @17:52:35.53 CSV.dial :flush @17:52:36.00 CS.reconfigure CSfast @17:52:36.05 CS.seek 10ml @17:52:59.14 delay 5 seconds @17:53:04.16 CSV.dial CBV @17:53:04.19
CBV.dial CSV @17:53:04.97 CTV.dial :intake @17:53:05.75 Intake.select :open @17:53:05.87 CS.seek 0ml @17:53:29.76 Intake.select :close @17:53:29.88 CTV.dial :air @17:53:30.64 Flushing upper manifold @17:53:30.78 CSV.dial :flush @17:53:31.56 CS.seek 5ml @17:53:43.80 delay 5 seconds @17:53:48.82 CSV.dial :air @17:53:49.48 CS.seek 7ml @17:53:54.92 CSV.dial CBV @17:53:54.94
CBV.dial CSV @17:53:55.98 CTV.dial :air @17:53:56.00 CS.seek 0ml @17:54:12.52 delay 7 seconds @17:54:19.54 CS.seek 3ml @17:54:27.26 CSV.dial :waste @17:54:27.74 CS.seek 0ml @17:54:35.64 CSV.dial :flush @17:54:36.12 CS.seek 3ml @17:54:43.80 delay 5 seconds @17:54:48.81 CSV.dial :air @17:54:49.35 CS.seek 5ml @17:54:54.82 CSV.dial :air @17:54:54.85 CS.seek 0ml @17:55:06.95 delay 7 seconds @17:55:14.58 CS.seek 3ml @17:55:22.38 CSV.dial :waste @17:55:23.15 CS.seek 0ml @17:55:30.83 Garaging FlushPuck in Collection Clamp @17:55:30.91 Elbow.reconfigure TightElbow @17:55:30.96 Elbow.seek CC @17:55:31.22 Elbow.reconfigure LooseElbow @17:55:31.33 Forearm.seek CC @17:55:32.32 Hand.close @17:55:32.91 CC.seek :open @17:55:37.30 Forearm.seek :retract @17:55:38.29 Elbow.reconfigure TightElbow @17:55:38.34 Elbow.seek :garage @17:55:41.45 Elbow.reconfigure LooseElbow @17:55:41.55 Forearm.reconfigure TightForearm @17:55:41.60 Forearm.seek :garage @17:55:42.78 Hand.open @17:55:43.48 Forearm.reconfigure LooseForearm @17:55:43.53 Forearm.seek :retract @17:55:44.56 Completed [phasewithdil] begun 14:00:23 @17:55:44.58 Preparing to sleep until 14PM @17:55:44.62 deferred until MFBPCR finish @17:55:44.64
Waiting for MFBPCR til 14PM @17:55:44.67 Continuing at 14:00CEST06-Oct-18 @17:55:44.74 GoodNews.email "Then, in tube 2: phasewithdil\n/home/esp/esp2/mission/18tundeployv2dil.rb:118\n{1 => 18, 2 => 4}\nCan@17:55:44, 15.2C, 67% humidity, 14.4psia, 13.672V, 0.809A, 0.809A avg, 11.06W",Subject: "Waiting until 14:00CEST06-Oct-18" @18:24:59.66 PCR.off @18:25:01.34 PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0516h1500ml.pcr @18:25:01.42 Cleaning PCR @18:25:01.43 AFV.select :carrierSolution @18:25:01.54 delay 2 seconds @18:25:03.57 AS.reconfigure ASconfig @18:25:03.62 AS.seek 0.5ml @18:25:13.95 delay 1 second @18:25:14.97 AFV.select :holdingCoil @18:25:15.08 ASV.dial :waste1 @18:25:15.45 ATV.dial :air3 @18:25:16.65 AS.reconfigure ASslow1 @18:25:16.70 AS.seek 0.51ml @18:25:19.00 delay 2 seconds @18:25:21.01 ATV.dial :bleach @18:25:21.62 AS.seek 0.81ml @18:26:22.20 delay 3 seconds @18:26:25.22 ATV.dial :air3 @18:26:25.75 delay 3 seconds @18:26:28.84 AS.seek 0.835ml @18:26:34.12 delay 3 seconds @18:26:37.14 ATV.dial :bleach @18:26:37.80 AS.seek 0.82ml @18:26:41.09 delay 3 seconds @18:26:44.11 ARV.dial :waste @18:26:44.70 delay 3 seconds @18:26:47.72 ATV.dial :ARV @18:26:48.66 AS.reconfigure ASconfig @18:26:48.71 AS.seek 0.47ml @18:27:09.11 AFV.select :carrierSolution @18:27:09.23 delay 2 seconds @18:27:11.26 AS.seek 1ml @18:27:42.02 delay 1 second @18:27:43.03 AFV.select :holdingCoil @18:27:43.14 delay 1 second @18:27:44.17 AS.reconfigure ASfast @18:27:44.22 AS.seek 0ml @18:28:11.57 AFV.select :carrierSolution @18:28:11.69 delay 2 seconds @18:28:13.72 AS.reconfigure ASconfig @18:28:13.77 AS.seek 1ml @18:29:11.56 delay 1 second @18:29:12.57 AFV.select :holdingCoil @18:29:12.68 delay 1 second @18:29:13.71 AS.reconfigure ASfast @18:29:13.76 AS.seek 0ml @18:29:41.08 AFV.select :carrierSolution @18:29:41.20 delay 2 seconds @18:29:43.23 AS.reconfigure ASconfig @18:29:43.28 AS.seek 1ml @18:30:41.06 delay 1 second @18:30:42.07 AFV.select :holdingCoil @18:30:42.18 delay 1 second @18:30:43.21 AS.reconfigure ASfast @18:30:43.26 AS.seek 0ml @18:31:10.59 ARV.dial between carrier and air1 @18:31:11.06 ATV.dial :air3 @18:31:12.26 AS.reconfigure ASslow1 @18:31:12.31 AS.seek 0.01ml @18:31:14.59 delay 2 seconds @18:31:16.60 ATV.dial :bleach @18:31:17.20 AS.seek 0.81ml @18:33:58.44 delay 3 seconds @18:34:01.46 ATV.dial :air3 @18:34:01.99 delay 3 seconds @18:34:05.08 AS.seek 0.835ml @18:34:10.45 delay 3 seconds @18:34:13.47 ATV.dial :bleach @18:34:14.17 AS.seek 0.82ml @18:34:17.52 delay 3 seconds @18:34:20.54 ATV.dial PCR @18:34:20.92 AS.reconfigure ASconfig @18:34:20.97 AS.seek :empty @18:34:56.50 Session Begun @18:35:08.44 PCR.program :bleachProfile @18:35:08.51 PCR program should complete in < 2:45 @18:35:08.53 PCR.run 10:00 @18:35:23.01 -> define '18merMissionUpdate' @18:35:44.19 -> list Method 'phasewithdil' @18:35:44.22 NoMethodError in preston -- undefined method `Method' for # @18:36:12.85 -> list method 'phasewithdil' @18:36:26.27 -> exit @18:36:26.29 Session Ended @18:38:17.03 PCR.off @18:38:17.06 AFV.select :carrierSolution @18:38:17.17 delay 2 seconds @18:38:19.20 AS.seek 1ml @18:39:17.05 delay 1 second @18:39:18.06 AFV.select :holdingCoil @18:39:18.17 delay 1 second @18:39:19.20 AS.reconfigure ASfast @18:39:19.25 AS.seek 0ml @18:39:46.60 delay 3 seconds @18:39:49.62 AFV.select :carrierSolution @18:39:49.73 delay 2 seconds @18:39:51.76 AS.reconfigure ASconfig @18:39:51.82 AS.seek 1ml @18:40:49.60 delay 1 second @18:40:50.61 AFV.select :holdingCoil @18:40:50.72 delay 1 second @18:40:51.75 AS.reconfigure ASfast @18:40:51.80 AS.seek 0ml @18:41:19.16 delay 3 seconds @18:41:22.18 AFV.select :carrierSolution @18:41:22.29 delay 2 seconds @18:41:24.32 AS.reconfigure ASconfig @18:41:24.37 AS.seek 1ml @18:42:22.18 delay 1 second @18:42:23.20 AFV.select :holdingCoil @18:42:23.31 delay 1 second @18:42:24.34 AS.reconfigure ASfast @18:42:24.39 AS.seek 0ml @18:42:51.72 delay 3 seconds @18:42:54.74 AFV.select :carrierSolution @18:42:54.85 delay 2 seconds @18:42:56.88 AS.reconfigure ASconfig @18:42:56.94 AS.seek 1ml @18:43:54.74 delay 1 second @18:43:55.75 AFV.select :holdingCoil @18:43:55.86 delay 1 second @18:43:56.89 AS.reconfigure ASfast @18:43:56.94 AS.seek 0ml @18:44:24.30 delay 3 seconds @18:44:27.33 AFV.select :carrierSolution @18:44:27.43 delay 2 seconds @18:44:29.46 AS.reconfigure ASconfig @18:44:29.52 AS.seek 1ml @18:45:27.30 delay 1 second @18:45:28.31 AFV.select :holdingCoil @18:45:28.42 delay 1 second @18:45:29.45 AS.reconfigure ASnorm @18:45:29.50 AS.seek 0ml @18:46:20.20 Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0518h1500ml.pcr @18:46:20.22 Building PCR Slug from ASV with MM2 mix, PP3 primer @18:46:20.23 Priming with carrier @18:46:20.24 ATV.dial ARV @18:46:20.82 ARV.dial :carrier @18:46:21.17 AS.seek 0.5ml @18:46:46.79 AFV.select :carrierSolution @18:46:46.91 delay 2 seconds @18:46:48.94 AS.reconfigure ASconfig @18:46:48.99 AS.seek 1ml @18:47:18.01 delay 1 second @18:47:19.02 AFV.select :holdingCoil @18:47:19.13 delay 1 second @18:47:20.15 ATV.dial :waste @18:47:21.68 AS.reconfigure ASnorm @18:47:21.73 AS.seek :empty @18:48:12.45 AFV.select :carrierSolution @18:48:12.57 delay 2 seconds @18:48:14.60 AS.reconfigure ASconfig @18:48:14.65 AS.seek 0.5ml @18:48:43.79 delay 1 second @18:48:44.80 AFV.select :holdingCoil @18:48:44.91 Building Slug @18:48:44.92 ATV.dial :ARV @18:48:46.52 delay 2 seconds @18:48:48.54 ARV.dial :air1 @18:48:48.92 delay 2 seconds @18:48:51.00 AS.reconfigure ASslow2 @18:48:51.06 AS.seek 0.506ml @18:48:57.35 delay 2 seconds @18:48:59.36 ARV.dial :MM2 @18:49:00.76 delay 2 seconds @18:49:02.79 Adding MM2 @18:49:02.87 AS.seek 0.524ml @18:49:21.16 delay 25 seconds @18:49:46.18 ARV.dial :PP3 @18:49:46.57 Adding PP3 @18:49:46.58 delay 2 seconds @18:49:48.67 AS.seek 0.53ml @18:49:55.05 delay 10 seconds @18:50:05.07 ARV.dial :air2 @18:50:05.46 adding ARV air @18:50:05.47 delay 2 seconds @18:50:07.56 AS.seek 0.536ml @18:50:13.88 delay 5 seconds @18:50:18.90 ARV.dial :carrier @18:50:20.10 delay 7 seconds @18:50:27.19 AS.seek 0.594ml @18:51:26.08 delay 5 seconds @18:51:31.10 ATV.dial :ASV @18:51:31.51 delay 2 seconds @18:51:33.60 AS.seek 0.6ml @18:51:39.91 delay 4 seconds @18:51:43.93 ATV.dial ARV @18:51:44.55 delay 2 seconds @18:51:46.57 ARV.dial :carrier @18:51:46.59 delay 2 seconds @18:51:49.27 AS.seek 0.65ml @18:52:39.87 delay 4 seconds @18:52:43.89 ATV.dial PCR @18:52:44.48 ASV.dial :waste1 @18:52:44.49 ARV.dial :air1 @18:52:44.98 Positioning Slug by 0.324ml @18:52:45.07 AS.seek 0.326ml @18:58:10.90 delay 5 seconds @18:58:15.92 ATV.dial ARV @18:58:16.74 ASV.dial :PCRlock @18:58:17.12 delay 5 seconds @18:58:22.15 Running Rp Profile60C300 thermocycling @18:58:22.16 PCR.program Profile60C300 @18:58:22.30 Profile60C300 should complete in < 1:45:21 @18:58:22.31 PCR.run 2:16:41.25 @20:20:22.52 PCR.off @20:20:24.10 PCR for Rp completed -- closed /var/log/esp/Rp18oct0518h1500ml.pcr @20:20:24.18 Cleaning PCR @20:20:24.19 AFV.select :carrierSolution @20:20:24.30 delay 2 seconds @20:20:26.33 AS.reconfigure ASconfig @20:20:26.38 AS.seek 0.5ml @20:20:36.69 delay 1 second @20:20:37.70 AFV.select :holdingCoil @20:20:37.81 ASV.dial :waste1 @20:20:38.17 ATV.dial :air3 @20:20:39.35 AS.reconfigure ASslow1 @20:20:39.40 AS.seek 0.51ml @20:20:41.70 delay 2 seconds @20:20:43.72 ATV.dial :bleach @20:20:44.32 AS.seek 0.81ml @20:21:44.94 delay 3 seconds @20:21:47.96 ATV.dial :air3 @20:21:48.49 delay 3 seconds @20:21:51.58 AS.seek 0.835ml @20:21:56.90 delay 3 seconds @20:21:59.92 ATV.dial :bleach @20:22:00.53 AS.seek 0.82ml @20:22:03.83 delay 3 seconds @20:22:06.85 ARV.dial :waste @20:22:07.42 delay 3 seconds @20:22:10.44 ATV.dial :ARV @20:22:11.29 AS.reconfigure ASconfig @20:22:11.34 AS.seek 0.47ml @20:22:31.73 AFV.select :carrierSolution @20:22:31.85 delay 2 seconds @20:22:33.88 AS.seek 1ml @20:23:04.69 delay 1 second @20:23:05.70 AFV.select :holdingCoil @20:23:05.81 delay 1 second @20:23:06.84 AS.reconfigure ASfast @20:23:06.89 AS.seek 0ml @20:23:34.24 AFV.select :carrierSolution @20:23:34.36 delay 2 seconds @20:23:36.39 AS.reconfigure ASconfig @20:23:36.44 AS.seek 1ml @20:24:34.22 delay 1 second @20:24:35.23 AFV.select :holdingCoil @20:24:35.34 delay 1 second @20:24:36.37 AS.reconfigure ASfast @20:24:36.42 AS.seek 0ml @20:25:03.74 AFV.select :carrierSolution @20:25:03.86 delay 2 seconds @20:25:05.89 AS.reconfigure ASconfig @20:25:05.94 AS.seek 1ml @20:26:03.71 delay 1 second @20:26:04.72 AFV.select :holdingCoil @20:26:04.83 delay 1 second @20:26:05.86 AS.reconfigure ASfast @20:26:05.91 AS.seek 0ml @20:26:33.24 ARV.dial between carrier and air1 @20:26:33.72 ATV.dial :air3 @20:26:34.93 AS.reconfigure ASslow1 @20:26:34.98 AS.seek 0.01ml @20:26:37.35 delay 2 seconds @20:26:39.36 ATV.dial :bleach @20:26:39.96 AS.seek 0.81ml @20:29:21.16 delay 3 seconds @20:29:24.18 ATV.dial :air3 @20:29:24.71 delay 3 seconds @20:29:27.80 AS.seek 0.835ml @20:29:33.08 delay 3 seconds @20:29:36.10 ATV.dial :bleach @20:29:36.70 AS.seek 0.82ml @20:29:39.99 delay 3 seconds @20:29:43.01 ATV.dial PCR @20:29:43.40 AS.reconfigure ASconfig @20:29:43.45 AS.seek :empty @20:30:30.93 PCR.program :bleachProfile @20:30:31.00 PCR program should complete in < 2:45 @20:30:31.01 PCR.run 10:00 @20:33:39.46 PCR.off @20:33:39.48 AFV.select :carrierSolution @20:33:39.60 delay 2 seconds @20:33:41.63 AS.seek 1ml @20:34:39.52 delay 1 second @20:34:40.53 AFV.select :holdingCoil @20:34:40.64 delay 1 second @20:34:41.67 AS.reconfigure ASfast @20:34:41.72 AS.seek 0ml @20:35:09.07 delay 3 seconds @20:35:12.09 AFV.select :carrierSolution @20:35:12.20 delay 2 seconds @20:35:14.23 AS.reconfigure ASconfig @20:35:14.28 AS.seek 1ml @20:36:12.08 delay 1 second @20:36:13.09 AFV.select :holdingCoil @20:36:13.20 delay 1 second @20:36:14.23 AS.reconfigure ASfast @20:36:14.28 AS.seek 0ml @20:36:41.62 delay 3 seconds @20:36:44.64 AFV.select :carrierSolution @20:36:44.75 delay 2 seconds @20:36:46.78 AS.reconfigure ASconfig @20:36:46.83 AS.seek 1ml @20:37:44.59 delay 1 second @20:37:45.60 AFV.select :holdingCoil @20:37:45.71 delay 1 second @20:37:46.74 AS.reconfigure ASfast @20:37:46.79 AS.seek 0ml @20:38:14.13 delay 3 seconds @20:38:17.15 AFV.select :carrierSolution @20:38:17.26 delay 2 seconds @20:38:19.29 AS.reconfigure ASconfig @20:38:19.34 AS.seek 1ml @20:39:17.12 delay 1 second @20:39:18.13 AFV.select :holdingCoil @20:39:18.24 delay 1 second @20:39:19.27 AS.reconfigure ASfast @20:39:19.32 AS.seek 0ml @20:39:46.65 delay 3 seconds @20:39:49.67 AFV.select :carrierSolution @20:39:49.78 delay 2 seconds @20:39:51.81 AS.reconfigure ASconfig @20:39:51.87 AS.seek 1ml @20:40:49.66 delay 1 second @20:40:50.67 AFV.select :holdingCoil @20:40:50.78 delay 1 second @20:40:51.81 AS.reconfigure ASnorm @20:40:51.86 AS.seek 0ml @20:41:42.61 Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0520h1500ml.pcr @20:41:42.63 Building PCR Slug from ASV with MM3 mix, PP5 primer @20:41:42.64 Priming with carrier @20:41:42.65 ATV.dial ARV @20:41:43.23 ARV.dial :carrier @20:41:43.58 AS.seek 0.5ml @20:42:09.09 AFV.select :carrierSolution @20:42:09.21 delay 2 seconds @20:42:11.24 AS.reconfigure ASconfig @20:42:11.29 AS.seek 1ml @20:42:40.31 delay 1 second @20:42:41.32 AFV.select :holdingCoil @20:42:41.43 delay 1 second @20:42:42.45 ATV.dial :waste @20:42:43.98 AS.reconfigure ASnorm @20:42:44.03 AS.seek :empty @20:43:34.76 AFV.select :carrierSolution @20:43:34.88 delay 2 seconds @20:43:36.91 AS.reconfigure ASconfig @20:43:36.96 AS.seek 0.5ml @20:44:06.04 delay 1 second @20:44:07.05 AFV.select :holdingCoil @20:44:07.16 Building Slug @20:44:07.17 ATV.dial :ARV @20:44:08.77 delay 2 seconds @20:44:10.79 ARV.dial :air1 @20:44:11.18 delay 2 seconds @20:44:13.26 AS.reconfigure ASslow2 @20:44:13.31 AS.seek 0.506ml @20:44:19.67 delay 2 seconds @20:44:21.68 ARV.dial :MM3 @20:44:22.67 delay 2 seconds @20:44:24.70 Adding MM3 @20:44:24.78 AS.seek 0.524ml @20:44:43.15 delay 25 seconds @20:45:08.17 ARV.dial :PP5 @20:45:08.56 Adding PP5 @20:45:08.57 delay 2 seconds @20:45:10.66 AS.seek 0.53ml @20:45:16.93 delay 10 seconds @20:45:26.96 ARV.dial :air3 @20:45:27.32 adding ARV air @20:45:27.33 delay 2 seconds @20:45:29.42 AS.seek 0.536ml @20:45:35.65 delay 5 seconds @20:45:40.67 ARV.dial :carrier @20:45:41.87 delay 7 seconds @20:45:48.96 AS.seek 0.594ml @20:46:47.62 delay 5 seconds @20:46:52.64 ATV.dial :ASV @20:46:53.64 delay 2 seconds @20:46:55.73 AS.seek 0.6ml @20:47:02.07 delay 4 seconds @20:47:06.09 ATV.dial ARV @20:47:06.71 delay 2 seconds @20:47:08.73 ARV.dial :carrier @20:47:08.75 delay 2 seconds @20:47:10.84 AS.seek 0.65ml @20:48:01.39 delay 4 seconds @20:48:05.42 ATV.dial PCR @20:48:06.01 ASV.dial :waste1 @20:48:06.02 ARV.dial :air1 @20:48:06.41 Positioning Slug by 0.324ml @20:48:06.50 AS.seek 0.326ml @20:53:32.54 delay 5 seconds @20:53:37.56 ATV.dial ARV @20:53:38.28 ASV.dial :PCRlock @20:53:38.65 delay 5 seconds @20:53:43.68 Running Bb Profile60C300 thermocycling @20:53:43.69 PCR.program Profile60C300 @20:53:43.83 Profile60C300 should complete in < 1:45:21 @20:53:43.84 PCR.run 2:16:41.25 @22:15:32.90 PCR.off @22:15:34.49 PCR for Bb completed -- closed /var/log/esp/Bb18oct0520h1500ml.pcr @22:15:34.57 Cleaning PCR @22:15:34.58 AFV.select :carrierSolution @22:15:34.69 delay 2 seconds @22:15:36.72 AS.reconfigure ASconfig @22:15:36.77 AS.seek 0.5ml @22:15:47.07 delay 1 second @22:15:48.08 AFV.select :holdingCoil @22:15:48.19 ASV.dial :waste1 @22:15:48.55 ATV.dial :air3 @22:15:49.75 AS.reconfigure ASslow1 @22:15:49.80 AS.seek 0.51ml @22:15:52.11 delay 2 seconds @22:15:54.12 ATV.dial :bleach @22:15:54.72 AS.seek 0.81ml @22:16:55.30 delay 3 seconds @22:16:58.32 ATV.dial :air3 @22:16:58.85 delay 3 seconds @22:17:01.94 AS.seek 0.835ml @22:17:07.23 delay 3 seconds @22:17:10.25 ATV.dial :bleach @22:17:10.86 AS.seek 0.82ml @22:17:14.16 delay 3 seconds @22:17:17.18 ARV.dial :waste @22:17:17.76 delay 3 seconds @22:17:20.78 ATV.dial :ARV @22:17:21.64 AS.reconfigure ASconfig @22:17:21.69 AS.seek 0.47ml @22:17:42.11 AFV.select :carrierSolution @22:17:42.23 delay 2 seconds @22:17:44.26 AS.seek 1ml @22:18:15.04 delay 1 second @22:18:16.05 AFV.select :holdingCoil @22:18:16.16 delay 1 second @22:18:17.19 AS.reconfigure ASfast @22:18:17.24 AS.seek 0ml @22:18:44.56 AFV.select :carrierSolution @22:18:44.68 delay 2 seconds @22:18:46.71 AS.reconfigure ASconfig @22:18:46.76 AS.seek 1ml @22:19:44.54 delay 1 second @22:19:45.55 AFV.select :holdingCoil @22:19:45.66 delay 1 second @22:19:46.69 AS.reconfigure ASfast @22:19:46.74 AS.seek 0ml @22:20:14.09 AFV.select :carrierSolution @22:20:14.21 delay 2 seconds @22:20:16.24 AS.reconfigure ASconfig @22:20:16.29 AS.seek 1ml @22:21:14.05 delay 1 second @22:21:15.06 AFV.select :holdingCoil @22:21:15.17 delay 1 second @22:21:16.20 AS.reconfigure ASfast @22:21:16.25 AS.seek 0ml @22:21:43.58 ARV.dial between carrier and air1 @22:21:44.07 ATV.dial :air3 @22:21:45.28 AS.reconfigure ASslow1 @22:21:45.33 AS.seek 0.01ml @22:21:47.65 delay 2 seconds @22:21:49.66 ATV.dial :bleach @22:21:50.27 AS.seek 0.81ml @22:24:31.48 delay 3 seconds @22:24:34.50 ATV.dial :air3 @22:24:35.02 delay 3 seconds @22:24:38.11 AS.seek 0.835ml @22:24:43.35 delay 3 seconds @22:24:46.37 ATV.dial :bleach @22:24:46.97 AS.seek 0.82ml @22:24:50.29 delay 3 seconds @22:24:53.32 ATV.dial PCR @22:24:53.73 AS.reconfigure ASconfig @22:24:53.78 AS.seek :empty @22:25:41.27 PCR.program :bleachProfile @22:25:41.33 PCR program should complete in < 2:45 @22:25:41.34 PCR.run 10:00 @22:28:49.22 PCR.off @22:28:49.25 AFV.select :carrierSolution @22:28:49.36 delay 2 seconds @22:28:51.39 AS.seek 1ml @22:29:49.23 delay 1 second @22:29:50.24 AFV.select :holdingCoil @22:29:50.35 delay 1 second @22:29:51.38 AS.reconfigure ASfast @22:29:51.43 AS.seek 0ml @22:30:18.81 delay 3 seconds @22:30:21.83 AFV.select :carrierSolution @22:30:21.94 delay 2 seconds @22:30:23.97 AS.reconfigure ASconfig @22:30:24.02 AS.seek 1ml @22:31:21.84 delay 1 second @22:31:22.86 AFV.select :holdingCoil @22:31:22.97 delay 1 second @22:31:24.00 AS.reconfigure ASfast @22:31:24.05 AS.seek 0ml @22:31:51.40 delay 3 seconds @22:31:54.42 AFV.select :carrierSolution @22:31:54.53 delay 2 seconds @22:31:56.56 AS.reconfigure ASconfig @22:31:56.62 AS.seek 1ml @22:32:54.46 delay 1 second @22:32:55.47 AFV.select :holdingCoil @22:32:55.58 delay 1 second @22:32:56.61 AS.reconfigure ASfast @22:32:56.66 AS.seek 0ml @22:33:24.01 delay 3 seconds @22:33:27.03 AFV.select :carrierSolution @22:33:27.14 delay 2 seconds @22:33:29.17 AS.reconfigure ASconfig @22:33:29.22 AS.seek 1ml @22:34:27.03 delay 1 second @22:34:28.04 AFV.select :holdingCoil @22:34:28.15 delay 1 second @22:34:29.18 AS.reconfigure ASfast @22:34:29.23 AS.seek 0ml @22:34:56.57 delay 3 seconds @22:34:59.59 AFV.select :carrierSolution @22:34:59.70 delay 2 seconds @22:35:01.73 AS.reconfigure ASconfig @22:35:01.79 AS.seek 1ml @22:35:59.59 delay 1 second @22:36:00.60 AFV.select :holdingCoil @22:36:00.71 delay 1 second @22:36:01.74 AS.reconfigure ASnorm @22:36:01.79 AS.seek 0ml @22:36:52.52 Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0522h1500ml.pcr @22:36:52.54 Building PCR Slug from ASV with MM1 mix, PP2 primer @22:36:52.55 Priming with carrier @22:36:52.56 ATV.dial ARV @22:36:53.25 ARV.dial :carrier @22:36:53.61 AS.seek 0.5ml @22:37:19.16 AFV.select :carrierSolution @22:37:19.28 delay 2 seconds @22:37:21.31 AS.reconfigure ASconfig @22:37:21.36 AS.seek 1ml @22:37:50.43 delay 1 second @22:37:51.45 AFV.select :holdingCoil @22:37:51.56 delay 1 second @22:37:52.58 ATV.dial :waste @22:37:54.11 AS.reconfigure ASnorm @22:37:54.16 AS.seek :empty @22:38:44.88 AFV.select :carrierSolution @22:38:45.00 delay 2 seconds @22:38:47.03 AS.reconfigure ASconfig @22:38:47.08 AS.seek 0.5ml @22:39:16.18 delay 1 second @22:39:17.20 AFV.select :holdingCoil @22:39:17.31 Building Slug @22:39:17.32 ATV.dial :ARV @22:39:18.92 delay 2 seconds @22:39:20.94 ARV.dial :air1 @22:39:21.34 delay 2 seconds @22:39:23.42 AS.reconfigure ASslow2 @22:39:23.48 AS.seek 0.506ml @22:39:29.69 delay 2 seconds @22:39:31.71 ARV.dial :MM1 @22:39:32.29 delay 2 seconds @22:39:34.32 Adding MM1 @22:39:34.39 AS.seek 0.524ml @22:39:52.86 delay 25 seconds @22:40:17.88 ARV.dial :PP2 @22:40:18.26 Adding PP2 @22:40:18.27 delay 2 seconds @22:40:20.36 AS.seek 0.53ml @22:40:26.92 delay 10 seconds @22:40:36.94 ARV.dial :air2 @22:40:37.31 adding ARV air @22:40:37.32 delay 2 seconds @22:40:40.01 AS.seek 0.536ml @22:40:46.22 delay 5 seconds @22:40:51.24 ARV.dial :carrier @22:40:52.44 delay 7 seconds @22:40:59.54 AS.seek 0.594ml @22:41:58.09 delay 5 seconds @22:42:03.11 ATV.dial :ASV @22:42:03.51 delay 2 seconds @22:42:05.60 AS.seek 0.6ml @22:42:11.75 delay 4 seconds @22:42:15.77 ATV.dial ARV @22:42:16.40 delay 2 seconds @22:42:18.42 ARV.dial :carrier @22:42:18.44 delay 2 seconds @22:42:20.53 AS.seek 0.65ml @22:43:10.97 delay 4 seconds @22:43:14.99 ATV.dial PCR @22:43:15.59 ASV.dial :waste1 @22:43:15.60 ARV.dial :air1 @22:43:15.99 Positioning Slug by 0.324ml @22:43:16.08 AS.seek 0.326ml @22:48:42.34 delay 5 seconds @22:48:47.36 ATV.dial ARV @22:48:48.09 ASV.dial :PCRlock @22:48:48.45 delay 5 seconds @22:48:53.48 Running Ss Profile60C350 thermocycling @22:48:53.49 PCR.program Profile60C350 @22:48:53.64 Profile60C350 should complete in < 1:48:05 @22:48:53.65 PCR.run 2:20:06.25 @00:11:19.50CEST06-Oct-18 PCR.off @00:11:21.59 PCR for Ss completed -- closed /var/log/esp/Ss18oct0522h1500ml.pcr @00:11:21.66 Cleaning PCR @00:11:21.67 AFV.select :carrierSolution @00:11:21.79 delay 2 seconds @00:11:23.82 AS.reconfigure ASconfig @00:11:23.87 AS.seek 0.5ml @00:11:34.15 delay 1 second @00:11:35.16 AFV.select :holdingCoil @00:11:35.27 ASV.dial :waste1 @00:11:35.64 ATV.dial :air3 @00:11:36.83 AS.reconfigure ASslow1 @00:11:36.88 AS.seek 0.51ml @00:11:39.19 delay 2 seconds @00:11:41.20 ATV.dial :bleach @00:11:41.80 AS.seek 0.81ml @00:12:42.37 delay 3 seconds @00:12:45.39 ATV.dial :air3 @00:12:45.92 delay 3 seconds @00:12:49.01 AS.seek 0.835ml @00:12:54.30 delay 3 seconds @00:12:57.32 ATV.dial :bleach @00:12:57.93 AS.seek 0.82ml @00:13:01.25 delay 3 seconds @00:13:04.27 ARV.dial :waste @00:13:04.87 delay 3 seconds @00:13:07.89 ATV.dial :ARV @00:13:08.74 AS.reconfigure ASconfig @00:13:08.79 AS.seek 0.47ml @00:13:29.19 AFV.select :carrierSolution @00:13:29.31 delay 2 seconds @00:13:31.34 AS.seek 1ml @00:14:02.12 delay 1 second @00:14:03.13 AFV.select :holdingCoil @00:14:03.24 delay 1 second @00:14:04.27 AS.reconfigure ASfast @00:14:04.32 AS.seek 0ml @00:14:31.67 AFV.select :carrierSolution @00:14:31.79 delay 2 seconds @00:14:33.82 AS.reconfigure ASconfig @00:14:33.87 AS.seek 1ml @00:15:31.62 delay 1 second @00:15:32.63 AFV.select :holdingCoil @00:15:32.74 delay 1 second @00:15:33.77 AS.reconfigure ASfast @00:15:33.83 AS.seek 0ml @00:16:01.17 AFV.select :carrierSolution @00:16:01.29 delay 2 seconds @00:16:03.32 AS.reconfigure ASconfig @00:16:03.37 AS.seek 1ml @00:17:01.15 delay 1 second @00:17:02.16 AFV.select :holdingCoil @00:17:02.27 delay 1 second @00:17:03.30 AS.reconfigure ASfast @00:17:03.92 AS.seek 0ml @00:17:31.28 ARV.dial between carrier and air1 @00:17:31.76 ATV.dial :air3 @00:17:32.96 AS.reconfigure ASslow1 @00:17:33.01 AS.seek 0.01ml @00:17:35.39 delay 2 seconds @00:17:37.41 ATV.dial :bleach @00:17:38.03 AS.seek 0.81ml @00:20:19.17 delay 3 seconds @00:20:22.19 ATV.dial :air3 @00:20:22.72 delay 3 seconds @00:20:25.81 AS.seek 0.835ml @00:20:31.12 delay 3 seconds @00:20:34.14 ATV.dial :bleach @00:20:34.74 AS.seek 0.82ml @00:20:38.08 delay 3 seconds @00:20:41.10 ATV.dial PCR @00:20:41.50 AS.reconfigure ASconfig @00:20:41.55 AS.seek :empty @00:21:29.00 PCR.program :bleachProfile @00:21:29.06 PCR program should complete in < 2:45 @00:21:29.07 PCR.run 10:00 @00:24:36.95 PCR.off @00:24:36.97 AFV.select :carrierSolution @00:24:37.08 delay 2 seconds @00:24:39.11 AS.seek 1ml @00:25:36.94 delay 1 second @00:25:37.95 AFV.select :holdingCoil @00:25:38.06 delay 1 second @00:25:39.09 AS.reconfigure ASfast @00:25:39.14 AS.seek 0ml @00:26:06.48 delay 3 seconds @00:26:09.50 AFV.select :carrierSolution @00:26:09.61 delay 2 seconds @00:26:11.64 AS.reconfigure ASconfig @00:26:11.70 AS.seek 1ml @00:27:09.47 delay 1 second @00:27:10.48 AFV.select :holdingCoil @00:27:10.59 delay 1 second @00:27:11.62 AS.reconfigure ASfast @00:27:11.67 AS.seek 0ml @00:27:39.03 delay 3 seconds @00:27:42.05 AFV.select :carrierSolution @00:27:42.16 delay 2 seconds @00:27:44.19 AS.reconfigure ASconfig @00:27:44.24 AS.seek 1ml @00:28:42.05 delay 1 second @00:28:43.07 AFV.select :holdingCoil @00:28:43.18 delay 1 second @00:28:44.21 AS.reconfigure ASfast @00:28:44.26 AS.seek 0ml @00:29:11.59 delay 3 seconds @00:29:14.61 AFV.select :carrierSolution @00:29:14.72 delay 2 seconds @00:29:16.75 AS.reconfigure ASconfig @00:29:16.81 AS.seek 1ml @00:30:14.59 delay 1 second @00:30:15.60 AFV.select :holdingCoil @00:30:15.71 delay 1 second @00:30:16.75 AS.reconfigure ASfast @00:30:16.80 AS.seek 0ml @00:30:44.13 delay 3 seconds @00:30:47.15 AFV.select :carrierSolution @00:30:47.26 delay 2 seconds @00:30:49.29 AS.reconfigure ASconfig @00:30:49.34 AS.seek 1ml @00:31:47.13 delay 1 second @00:31:48.14 AFV.select :holdingCoil @00:31:48.25 delay 1 second @00:31:49.28 AS.reconfigure ASnorm @00:31:49.33 AS.seek 0ml @00:32:40.07 Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0600h1500ml.pcr @00:32:40.09 Building PCR Slug from ASV with MM1 mix, PP1 primer @00:32:40.10 Priming with carrier @00:32:40.11 ATV.dial ARV @00:32:40.80 ARV.dial :carrier @00:32:41.15 AS.seek 0.5ml @00:33:06.71 AFV.select :carrierSolution @00:33:06.83 delay 2 seconds @00:33:08.86 AS.reconfigure ASconfig @00:33:08.91 AS.seek 1ml @00:33:37.97 delay 1 second @00:33:38.99 AFV.select :holdingCoil @00:33:39.10 delay 1 second @00:33:40.12 ATV.dial :waste @00:33:41.66 AS.reconfigure ASnorm @00:33:41.71 AS.seek :empty @00:34:32.45 AFV.select :carrierSolution @00:34:32.57 delay 2 seconds @00:34:34.60 AS.reconfigure ASconfig @00:34:34.65 AS.seek 0.5ml @00:35:03.72 delay 1 second @00:35:04.73 AFV.select :holdingCoil @00:35:04.84 Building Slug @00:35:04.85 ATV.dial :ARV @00:35:06.46 delay 2 seconds @00:35:09.14 ARV.dial :air1 @00:35:09.53 delay 2 seconds @00:35:11.62 AS.reconfigure ASslow2 @00:35:11.68 AS.seek 0.506ml @00:35:17.86 delay 2 seconds @00:35:19.89 ARV.dial :MM1 @00:35:20.46 delay 2 seconds @00:35:22.49 Adding MM1 @00:35:22.56 AS.seek 0.524ml @00:35:40.91 delay 25 seconds @00:36:05.93 ARV.dial :PP1 @00:36:06.33 Adding PP1 @00:36:06.34 delay 2 seconds @00:36:08.43 AS.seek 0.53ml @00:36:14.85 delay 10 seconds @00:36:24.87 ARV.dial :air1 @00:36:25.25 adding ARV air @00:36:25.27 delay 2 seconds @00:36:27.35 AS.seek 0.536ml @00:36:33.57 delay 5 seconds @00:36:38.59 ARV.dial :carrier @00:36:38.97 delay 7 seconds @00:36:46.06 AS.seek 0.594ml @00:37:44.61 delay 5 seconds @00:37:49.63 ATV.dial :ASV @00:37:50.03 delay 2 seconds @00:37:52.12 AS.seek 0.6ml @00:37:58.27 delay 4 seconds @00:38:02.29 ATV.dial ARV @00:38:02.82 delay 2 seconds @00:38:04.85 ARV.dial :carrier @00:38:04.86 delay 2 seconds @00:38:06.95 AS.seek 0.65ml @00:38:57.46 delay 4 seconds @00:39:01.48 ATV.dial PCR @00:39:02.07 ASV.dial :waste1 @00:39:02.08 ARV.dial :air1 @00:39:02.47 Positioning Slug by 0.324ml @00:39:02.56 AS.seek 0.326ml @00:44:28.79 delay 5 seconds @00:44:33.81 ATV.dial ARV @00:44:34.54 ASV.dial :PCRlock @00:44:34.91 delay 5 seconds @00:44:39.94 Running IPc ProfileIPc thermocycling @00:44:39.95 PCR.program ProfileIPc @00:44:40.11 ProfileIPc should complete in < 1:50:49 @00:44:40.12 PCR.run 2:23:31.25 @02:08:56.31 PCR.off @02:08:58.94 PCR for IPc completed -- closed /var/log/esp/IPc18oct0600h1500ml.pcr @02:08:58.96 Full MFB Decontamination @02:08:58.97 Decon extraction column and decon AIV @02:08:58.99 SPE.hold 2:46:40,55C @02:08:59.51 ASV.dial :SPEdelivery @02:09:00.28 delay 20 seconds @02:09:20.31 AFV.select :carrierSolution @02:09:20.42 delay 2 seconds @02:09:22.45 AS.reconfigure ASconfig @02:09:22.50 AS.seek 0.5ml @02:09:32.84 delay 1 second @02:09:33.86 AFV.select :holdingCoil @02:09:33.97 ATV.dial :air1 @02:09:35.66 AS.reconfigure ASslow1 @02:09:35.71 AS.seek 0.52ml @02:09:39.93 ATV.dial :ASV @02:09:41.50 ASV.dial :SPErecovery @02:09:42.13 AS.seek 0.62ml @02:10:02.51 ATV.dial :waste @02:10:04.27 AS.reconfigure ASnorm @02:10:04.33 AS.seek :empty @02:10:35.96 AFV.select :holdingCoil @02:10:35.98 ATV.dial :waste @02:10:36.00 AS.reconfigure ASconfig @02:10:36.05 AS.seek 0ml @02:10:36.38 AFV.select :carrierSolution @02:10:36.50 delay 2 seconds @02:10:38.53 AS.seek 0.5ml @02:11:07.55 delay 1 second @02:11:08.56 AFV.select :holdingCoil @02:11:08.67 ATV.dial :air3 @02:11:09.34 AS.reconfigure ASslow1 @02:11:09.39 AS.seek 0.52ml @02:11:13.69 ATV.dial :air3 @02:11:13.78 AS.seek 0.53ml @02:11:16.14 delay 2 seconds @02:11:18.16 ATV.dial :bleach @02:11:18.62 AS.seek 0.71ml @02:11:55.20 delay 3 seconds @02:11:58.22 ATV.dial :air3 @02:11:58.75 delay 3 seconds @02:12:01.84 AS.seek 0.735ml @02:12:07.19 delay 3 seconds @02:12:10.21 ATV.dial :bleach @02:12:10.81 AS.seek 0.72ml @02:12:14.14 delay 3 seconds @02:12:17.17 ATV.dial ASV @02:12:18.13 ASV.dial :SPErecovery @02:12:18.22 AS.seek 0.365ml @02:13:29.83 ATV.dial :waste @02:13:31.50 AS.reconfigure ASnorm @02:13:31.56 AS.seek :empty @02:13:50.30 ATV.dial :air3 @02:13:50.96 AS.reconfigure ASslow1 @02:13:51.01 AS.seek 0.009ml @02:13:53.39 delay 2 seconds @02:13:55.40 ATV.dial :bleach @02:13:55.87 AS.seek 0.579ml @02:15:50.77 delay 3 seconds @02:15:53.79 ATV.dial :air3 @02:15:54.31 delay 3 seconds @02:15:57.40 AS.seek 0.604ml @02:16:02.68 delay 3 seconds @02:16:05.70 ATV.dial :bleach @02:16:06.35 AS.seek 0.589ml @02:16:09.66 delay 3 seconds @02:16:12.68 ATV.dial :ASV @02:16:13.64 ASV.dial :wasteR @02:16:14.14 AS.seek 0ml @02:18:12.94 delay 1:00 @02:19:12.96 ATV.dial ASV @02:19:12.98 AFV.select :carrierSolution @02:19:13.08 delay 2 seconds @02:19:15.11 AS.reconfigure ASconfig @02:19:15.16 AS.seek 1ml @02:20:12.96 delay 1 second @02:20:13.97 AFV.select :holdingCoil @02:20:14.08 delay 1 second @02:20:15.10 ASV.dial :wasteR @02:20:15.12 AS.reconfigure ASslow1 @02:20:15.17 AS.seek 0ml @02:23:36.61 delay 10 seconds @02:23:46.64 ATV.dial :air3 @02:23:48.04 AS.seek 0.01ml @02:23:50.25 delay 2 seconds @02:23:52.26 ATV.dial :bleach @02:23:52.87 AS.seek 0.58ml @02:25:47.79 delay 3 seconds @02:25:50.81 ATV.dial :air3 @02:25:51.35 delay 3 seconds @02:25:55.04 AS.seek 0.605ml @02:26:00.37 delay 3 seconds @02:26:03.39 ATV.dial :bleach @02:26:04.01 AS.seek 0.59ml @02:26:07.32 delay 3 seconds @02:26:10.35 ATV.dial ASV @02:26:11.32 ASV.dial :SPEdelivery @02:26:11.69 AS.seek 0ml @02:28:10.65 delay 1:00 @02:29:10.67 AFV.select :carrierSolution @02:29:10.78 delay 2 seconds @02:29:12.81 AS.reconfigure ASconfig @02:29:12.86 AS.seek 1ml @02:30:10.63 delay 1 second @02:30:11.65 AFV.select :holdingCoil @02:30:11.76 delay 1 second @02:30:12.78 ATV.dial ASV @02:30:12.80 ASV.dial :SPEdelivery @02:30:12.82 AS.reconfigure ASslow1 @02:30:12.87 AS.seek 0ml @02:33:34.21 delay 10 seconds @02:33:44.24 ATV.dial :air2 @02:33:45.01 AS.reconfigure ASslow0 @02:33:45.07 AS.seek 0.8ml @02:35:05.83 delay 3 seconds @02:35:08.85 ATV.dial :ASV @02:35:09.77 ASV.dial :SPEdelivery @02:35:09.79 AS.reconfigure ASslow1 @02:35:09.84 AS.seek 0.03ml @02:37:44.96 delay 20 seconds @02:38:04.99 ATV.dial :waste,via: :air3 @02:38:06.59 AS.reconfigure ASconfig @02:38:06.64 AS.seek :empty @02:38:08.72 SPE.coast @02:38:08.82 Removing Bleach from Mixing Coil @02:38:08.84 AFV.select :carrierSolution @02:38:08.95 delay 2 seconds @02:38:10.99 AS.seek 0.5ml @02:38:40.09 delay 1 second @02:38:41.11 AFV.select :holdingCoil @02:38:41.22 ATV.dial :ASV @02:38:42.79 ASV.dial :SPErecovery @02:38:43.41 AS.reconfigure ASslow1 @02:38:43.46 AS.seek 0.73ml @02:39:30.09 ASV.dial :wasteD @02:39:30.65 AS.reconfigure ASnorm @02:39:30.71 AS.seek 0ml @02:40:07.80 AFV.select :carrierSolution @02:40:07.92 delay 2 seconds @02:40:09.95 AS.reconfigure ASconfig @02:40:10.00 AS.seek 0.5ml @02:40:39.05 delay 1 second @02:40:40.06 AFV.select :holdingCoil @02:40:40.17 ATV.dial ASV @02:40:40.18 ASV.dial :SPErecovery @02:40:40.81 AS.reconfigure ASslow0 @02:40:40.86 AS.seek 0.3ml @02:41:01.28 ATV.dial :air2 @02:41:02.28 AS.reconfigure ASslow1 @02:41:02.33 AS.seek 0.32ml @02:41:06.64 ATV.dial ASV @02:41:07.63 AS.reconfigure ASslow0 @02:41:07.68 AS.seek 0.57ml @02:41:33.14 ASV.dial :wasteD @02:41:33.79 AS.reconfigure ASnorm @02:41:33.85 AS.seek 0ml @02:42:02.92 AFV.select :carrierSolution @02:42:03.04 delay 2 seconds @02:42:05.07 AS.reconfigure ASconfig @02:42:05.12 AS.seek 0.5ml @02:42:34.16 delay 1 second @02:42:35.18 AFV.select :holdingCoil @02:42:35.29 ATV.dial ASV @02:42:35.30 ASV.dial :SPErecovery @02:42:35.92 AS.reconfigure ASslow0 @02:42:35.97 AS.seek 0.3ml @02:42:56.42 ATV.dial :air2 @02:42:57.42 AS.reconfigure ASslow1 @02:42:57.47 AS.seek 0.32ml @02:43:01.82 ATV.dial ASV @02:43:02.81 AS.reconfigure ASslow0 @02:43:02.86 AS.seek 0.57ml @02:43:28.33 ASV.dial :wasteD @02:43:28.90 AS.reconfigure ASnorm @02:43:28.95 AS.seek 0ml @02:43:58.01 ATV.dial :air2 @02:43:58.93 AS.reconfigure ASslow0 @02:43:58.99 AS.seek 0.3ml @02:44:29.51 ATV.dial :ASV @02:44:31.04 ASV.dial :wasteD @02:44:31.06 AS.reconfigure ASconfig @02:44:31.11 AS.seek 0.05ml @02:44:45.79 ATV.dial :waste @02:44:47.63 AS.seek 0ml @02:44:50.82 ASV.dial :SPEdelivery @02:44:51.76 deconLysateINT @02:44:51.77 ATV.dial :air3 @02:44:52.44 AS.reconfigure ASslow1 @02:44:52.49 AS.seek 0.01ml @02:44:54.90 delay 2 seconds @02:44:56.92 ATV.dial :bleach @02:44:57.38 AS.seek 0.96ml @02:48:08.78 delay 3 seconds @02:48:11.80 ATV.dial :air3 @02:48:12.34 delay 3 seconds @02:48:15.43 AS.seek 0.985ml @02:48:20.85 delay 3 seconds @02:48:23.87 ATV.dial :bleach @02:48:24.47 AS.seek 0.97ml @02:48:27.78 delay 3 seconds @02:48:30.81 ATV.dial AIV @02:48:31.98 AIV.dial :coilToMFB @02:48:31.99 AWV.select :waste @02:48:32.01 AFV.select :carrierSolution @02:48:32.12 delay 2 seconds @02:48:34.15 AS.reconfigure ASconfig @02:48:34.20 AS.seek 1ml @02:48:36.23 delay 1 second @02:48:37.24 AFV.select :holdingCoil @02:48:37.35 delay 1 second @02:48:38.38 AS.reconfigure ASslow0 @02:48:38.43 AS.seek :empty @02:50:19.42 AFV.select :carrierSolution @02:50:19.54 delay 2 seconds @02:50:21.57 AS.reconfigure ASconfig @02:50:21.62 AS.seek 1ml @02:51:19.48 delay 1 second @02:51:20.50 AFV.select :holdingCoil @02:51:20.61 delay 1 second @02:51:21.64 AS.reconfigure ASslow0 @02:51:21.69 AS.seek :empty @02:53:02.64 AFV.select :carrierSolution @02:53:02.76 delay 2 seconds @02:53:04.79 AS.reconfigure ASconfig @02:53:04.84 AS.seek 1ml @02:54:02.68 delay 1 second @02:54:03.69 AFV.select :holdingCoil @02:54:03.80 delay 1 second @02:54:04.83 AS.reconfigure ASslow0 @02:54:04.88 AS.seek :empty @02:55:45.87 ATV.dial :air2 @02:55:46.52 AS.reconfigure ASslow1 @02:55:46.57 AS.seek 0.009ml @02:55:48.89 AS.reconfigure ASslow0 @02:55:48.95 AS.seek 1ml @02:57:28.89 ATV.dial AIV @02:57:29.73 AS.seek 0.02ml @02:59:08.61 ATV.dial :waste @02:59:10.13 AS.seek 0ml @02:59:12.46 AWV.select :air @02:59:12.63 Cleaning PCR @02:59:12.64 AFV.select :carrierSolution @02:59:12.75 delay 2 seconds @02:59:14.78 AS.reconfigure ASconfig @02:59:14.83 AS.seek 0.5ml @02:59:43.86 delay 1 second @02:59:44.87 AFV.select :holdingCoil @02:59:44.98 ASV.dial :waste1 @02:59:45.34 ATV.dial :air3 @02:59:46.10 AS.reconfigure ASslow1 @02:59:46.15 AS.seek 0.51ml @02:59:48.50 delay 2 seconds @02:59:50.52 ATV.dial :bleach @02:59:50.97 AS.seek 0.81ml @03:00:51.56 delay 3 seconds @03:00:54.58 ATV.dial :air3 @03:00:55.11 delay 3 seconds @03:00:58.20 AS.seek 0.835ml @03:01:03.51 delay 3 seconds @03:01:06.53 ATV.dial :bleach @03:01:07.13 AS.seek 0.82ml @03:01:10.42 delay 3 seconds @03:01:13.44 ARV.dial :waste @03:01:14.01 delay 3 seconds @03:01:17.03 ATV.dial :ARV @03:01:17.88 AS.reconfigure ASconfig @03:01:17.93 AS.seek 0.47ml @03:01:38.32 AFV.select :carrierSolution @03:01:38.44 delay 2 seconds @03:01:40.47 AS.seek 1ml @03:02:11.20 delay 1 second @03:02:12.80 AFV.select :holdingCoil @03:02:12.93 delay 1 second @03:02:13.96 AS.reconfigure ASfast @03:02:14.01 AS.seek 0ml @03:02:41.35 AFV.select :carrierSolution @03:02:41.47 delay 2 seconds @03:02:43.50 AS.reconfigure ASconfig @03:02:43.55 AS.seek 1ml @03:03:41.34 delay 1 second @03:03:42.36 AFV.select :holdingCoil @03:03:42.47 delay 1 second @03:03:43.50 AS.reconfigure ASfast @03:03:43.55 AS.seek 0ml @03:04:10.88 AFV.select :carrierSolution @03:04:11.00 delay 2 seconds @03:04:13.03 AS.reconfigure ASconfig @03:04:13.08 AS.seek 1ml @03:05:10.86 delay 1 second @03:05:11.88 AFV.select :holdingCoil @03:05:11.99 delay 1 second @03:05:13.02 AS.reconfigure ASfast @03:05:13.07 AS.seek 0ml @03:05:40.39 ARV.dial between carrier and air1 @03:05:40.87 ATV.dial :air3 @03:05:42.08 AS.reconfigure ASslow1 @03:05:42.13 AS.seek 0.01ml @03:05:44.45 delay 2 seconds @03:05:46.47 ATV.dial :bleach @03:05:47.07 AS.seek 0.81ml @03:08:28.31 delay 3 seconds @03:08:31.33 ATV.dial :air3 @03:08:31.86 delay 3 seconds @03:08:34.95 AS.seek 0.835ml @03:08:40.27 delay 3 seconds @03:08:43.29 ATV.dial :bleach @03:08:43.90 AS.seek 0.82ml @03:08:47.22 delay 3 seconds @03:08:50.24 ATV.dial PCR @03:08:50.62 AS.reconfigure ASconfig @03:08:50.67 AS.seek :empty @03:09:38.16 PCR.program :bleachProfile @03:09:38.22 PCR program should complete in < 2:45 @03:09:38.23 PCR.run 10:00 @03:12:58.23 PCR.off @03:12:58.25 AFV.select :carrierSolution @03:12:58.37 delay 2 seconds @03:13:00.40 AS.seek 1ml @03:13:58.24 delay 1 second @03:13:59.25 AFV.select :holdingCoil @03:13:59.36 delay 1 second @03:14:00.39 AS.reconfigure ASfast @03:14:00.44 AS.seek 0ml @03:14:27.78 delay 3 seconds @03:14:30.80 AFV.select :carrierSolution @03:14:30.91 delay 2 seconds @03:14:32.95 AS.reconfigure ASconfig @03:14:32.99 AS.seek 1ml @03:15:30.78 delay 1 second @03:15:31.79 AFV.select :holdingCoil @03:15:31.90 delay 1 second @03:15:32.93 AS.reconfigure ASfast @03:15:32.98 AS.seek 0ml @03:16:00.30 delay 3 seconds @03:16:03.32 AFV.select :carrierSolution @03:16:03.43 delay 2 seconds @03:16:05.46 AS.reconfigure ASconfig @03:16:05.52 AS.seek 1ml @03:17:03.35 delay 1 second @03:17:04.36 AFV.select :holdingCoil @03:17:04.47 delay 1 second @03:17:05.50 AS.reconfigure ASfast @03:17:05.55 AS.seek 0ml @03:17:32.89 delay 3 seconds @03:17:35.91 AFV.select :carrierSolution @03:17:36.02 delay 2 seconds @03:17:38.05 AS.reconfigure ASconfig @03:17:38.10 AS.seek 1ml @03:18:35.94 delay 1 second @03:18:36.96 AFV.select :holdingCoil @03:18:37.07 delay 1 second @03:18:38.10 AS.reconfigure ASfast @03:18:38.15 AS.seek 0ml @03:19:05.60 delay 3 seconds @03:19:08.62 AFV.select :carrierSolution @03:19:08.73 delay 2 seconds @03:19:10.76 AS.reconfigure ASconfig @03:19:10.82 AS.seek 1ml @03:20:08.60 delay 1 second @03:20:09.61 AFV.select :holdingCoil @03:20:09.72 delay 1 second @03:20:10.75 AS.reconfigure ASnorm @03:20:10.80 AS.seek 0ml @03:21:01.51 AFV.select :carrierSolution @03:21:01.62 delay 2 seconds @03:21:03.65 AS.reconfigure ASconfig @03:21:03.70 AS.seek 1ml @03:22:01.50 delay 1 second @03:22:02.51 AFV.select :holdingCoil @03:22:02.62 delay 1 second @03:22:03.64 ATV.dial :waste @03:22:05.30 AS.reconfigure ASnorm @03:22:05.43 AS.seek 0.5ml @03:22:30.98 ATV.dial :waste2 @03:22:32.19 AS.seek 0ml @03:22:57.71 AS.coast @03:22:57.76 SS.coast @03:22:57.80 CC.coast @03:22:57.84 CS.coast @03:22:57.88 PC.coast @03:22:57.92 PS.coast @03:22:57.96 Forearm.coast @03:22:58.01 Elbow.coast @03:22:58.40 Gate.power :core,:OFF @03:22:58.42 Gate.power :raw,:OFF @03:22:58.43 Shutdown @03:22:58.44 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @08:23:09.98 Session Begun @08:23:15.38 -> showlog 200 @08:23:23.56 -> Time.now @08:23:41.25 -> $dilmfb @08:24:26.85 -> execute '18merMissionUpdate2' @08:24:39.62 -> $dilmfb @08:25:21.17 -> $dilmfb[:dilution]=0.1 @08:25:24.70 -> $delmfb @08:25:29.51 -> $dilmfb @08:28:46.05 -> can @08:33:34.82 -> exit @08:33:34.84 Session Ended @09:29:27.29 Session Begun @09:29:33.87 -> ESP.wakeup @09:29:33.92 Powering core bus @09:29:38.76 Gate.power :raw,:ON @09:29:39.56 SPE.coast @09:29:39.65 SPE.configure SPEconfig @09:29:39.77 AS.configure ASconfig @09:29:39.92 SS.configure SSconfig @09:29:40.04 CC.configure CCconfig @09:29:40.17 CS.configure CSconfig @09:29:40.30 PC.configure PCconfig @09:29:40.42 PS.configure PSconfig @09:29:40.58 Forearm.configure LooseForearm @09:29:40.71 Elbow.configure LooseElbow @09:29:40.86 SC.configure SCloose @09:29:40.98 SE.configure SEconfig @09:29:41.05 CH.coast @09:29:41.15 CH.configure CHconfig @09:29:41.20 PH.coast @09:29:41.30 PH.configure PHconfig @09:29:41.37 PCR.off @09:29:41.41 PCR.pidtemp= 50,0.1,0,300,25,25 @09:29:41.46 PCR.caltemp= 2.7073,0.0373,0 @09:29:41.51 All dwarf controllers running firmware version 3.75 @09:29:41.65 Valve.reset @09:29:41.84 PCR.off @09:29:41.89 PCR.pidtemp= 50,0.1,0,300,25,25 @09:29:41.93 PCR.caltemp= 2.7073,0.0373,0 @09:29:42.05 Intake.select :close @09:29:42.16 Exhaust.select :close @09:29:42.19 CTV.dial :air @09:29:42.30 PSV.dial :air @09:29:42.34 CSV.dial :waste1 @09:29:42.53 SSV.dial :spit,avoiding: :exhaust @09:29:42.57 Forearm.reconfigure LooseForearm @09:29:42.71 Forearm.home.seek :retract @09:29:42.90 PS.reconfigure PSconfig @09:29:42.97 PS.home.seek 0ml @09:29:43.53 Hand.close @09:29:43.57 Elbow.jog 1500 @09:29:43.72 CS.reconfigure CSfast @09:29:43.75 SS.reconfigure SSconfig @09:29:43.88 CS.home.seek 0ml @09:29:43.91 SS.home.seek 0ml @09:29:44.35 Elbow.reconfigure LooseElbow @09:29:44.42 Elbow.home.seek :clear @09:29:48.37 SE.reconfigure SEconfig @09:29:48.45 SE.home.seek :down @09:29:49.22 SC.reconfigure SCsloppy @09:29:49.30 SC.home.seek :initial @09:29:51.52 Forearm.seek :retract @09:29:51.64 Elbow.seek :hammer @09:29:52.06 PS.coast @09:29:52.87 Forearm.reconfigure TightForearm @09:29:52.95 Forearm.seek 5 + 350 counts @09:29:54.06 Forearm.seek :retract @09:29:55.28 CC.reconfigure CCconfig @09:29:55.30 PC.reconfigure PCconfig @09:29:55.43 CC.home.seek :open @09:29:55.46 PC.home.seek :open @09:30:21.94 -> ESP.ready? @09:30:29.79 -> MFB.ready? @09:30:58.98 -> MFB.ready! @09:30:59.08 Readying the MFB @09:30:59.10 Homing MFB Syringe @09:30:59.12 AFV.select :holdingCoil @09:30:59.31 ATV.dial :air2 @09:30:59.87 AS.reconfigure ASconfig @09:30:59.95 AS.home.seek :home @09:31:05.48 AFV.select :carrierSolution @09:31:05.62 delay 2 seconds @09:31:07.65 AS.seek 1ml @09:32:00.62 delay 1 second @09:32:01.63 AFV.select :holdingCoil @09:32:01.76 delay 1 second @09:32:02.79 ATV.dial :waste @09:32:03.80 AS.reconfigure ASnorm @09:32:03.88 AS.home.seek :empty @09:32:56.47 ATV.dial :waste @09:32:56.49 ARV.dial between carrier and air1 @09:32:56.62 ASV.dial :waste1 @09:33:04.38 -> ATV.ARV @09:33:04.41 ATV.dial ARV @09:33:10.32 -> ARV.air3 @09:33:10.34 ARV.dial :air3 @09:33:22.15 -> AS.pull 0.2, ASslow1 @09:33:22.26 AS.reconfigure ASslow1 @09:33:22.34 AS.seek 0.199ml @09:34:08.44 -> ATV @09:34:18.70 -> ARV.PP5 @09:34:18.72 ARV.dial :PP5 @09:34:22.75 -> ATV @09:34:25.92 -> ARV @09:34:40.30 -> AS.pull 0.05, ASslow1 @09:34:40.41 AS.seek 0.249ml @09:34:55.87 -> ATV @09:35:00.51 -> ARV.air3 @09:35:00.53 ARV.dial :air3 @09:35:15.74 -> AS.pull 0.2, ASslow1 @09:35:15.85 AS.seek 0.449ml @09:36:06.25 -> ATV.waste @09:36:06.27 ATV.dial :waste @09:36:11.19 -> AS.empty @09:36:11.22 AS.reconfigure ASconfig @09:36:11.31 AS.seek :empty @09:36:47.88 -> pcrDecon @09:36:47.99 Cleaning PCR @09:36:48.00 AFV.select :carrierSolution @09:36:48.15 delay 2 seconds @09:36:50.18 AS.seek 0.5ml @09:37:19.27 delay 1 second @09:37:20.29 AFV.select :holdingCoil @09:37:20.42 ASV.dial :waste1 @09:37:20.43 ATV.dial :air3 @09:37:21.27 AS.reconfigure ASslow1 @09:37:21.34 AS.seek 0.51ml @09:37:23.65 delay 2 seconds @09:37:25.67 ATV.dial :bleach @09:37:26.14 AS.seek 0.81ml @09:38:26.74 delay 3 seconds @09:38:29.76 ATV.dial :air3 @09:38:30.31 delay 3 seconds @09:38:33.40 AS.seek 0.835ml @09:38:38.71 delay 3 seconds @09:38:41.73 ATV.dial :bleach @09:38:42.35 AS.seek 0.82ml @09:38:45.67 delay 3 seconds @09:38:49.16 ARV.dial :waste @09:38:50.26 delay 3 seconds @09:38:53.28 ATV.dial :ARV @09:38:54.14 AS.reconfigure ASconfig @09:38:54.21 AS.seek 0.47ml @09:39:14.64 AFV.select :carrierSolution @09:39:14.78 delay 2 seconds @09:39:16.81 AS.seek 1ml @09:39:47.57 delay 1 second @09:39:48.59 AFV.select :holdingCoil @09:39:48.73 delay 1 second @09:39:49.76 AS.reconfigure ASfast @09:39:49.83 AS.seek 0ml @09:40:17.21 AFV.select :carrierSolution @09:40:17.35 delay 2 seconds @09:40:19.38 AS.reconfigure ASconfig @09:40:19.46 AS.seek 1ml @09:41:17.28 delay 1 second @09:41:18.30 AFV.select :holdingCoil @09:41:18.43 delay 1 second @09:41:19.46 AS.reconfigure ASfast @09:41:19.53 AS.seek 0ml @09:41:46.88 AFV.select :carrierSolution @09:41:47.02 delay 2 seconds @09:41:49.05 AS.reconfigure ASconfig @09:41:49.12 AS.seek 1ml @09:42:46.98 delay 1 second @09:42:48.00 AFV.select :holdingCoil @09:42:48.13 delay 1 second @09:42:49.16 AS.reconfigure ASfast @09:42:49.23 AS.seek 0ml @09:43:16.60 ARV.dial between carrier and air1 @09:43:17.10 ATV.dial :air3 @09:43:18.33 AS.reconfigure ASslow1 @09:43:18.40 AS.seek 0.01ml @09:43:20.76 delay 2 seconds @09:43:22.78 ATV.dial :bleach @09:43:23.39 AS.seek 0.81ml @09:46:04.56 delay 3 seconds @09:46:07.58 ATV.dial :air3 @09:46:08.14 delay 3 seconds @09:46:11.23 AS.seek 0.835ml @09:46:16.58 delay 3 seconds @09:46:19.60 ATV.dial :bleach @09:46:20.22 AS.seek 0.82ml @09:46:23.64 delay 3 seconds @09:46:26.66 ATV.dial PCR @09:46:27.07 AS.reconfigure ASconfig @09:46:27.14 AS.seek :empty @09:47:14.64 PCR.program :bleachProfile @09:47:14.71 PCR program should complete in < 2:45 @09:47:14.72 PCR.run 10:00 @09:50:46.87 PCR.off @09:50:46.91 AFV.select :carrierSolution @09:50:47.04 delay 2 seconds @09:50:49.07 AS.seek 1ml @09:51:46.93 delay 1 second @09:51:47.94 AFV.select :holdingCoil @09:51:48.07 delay 1 second @09:51:49.10 AS.reconfigure ASfast @09:51:49.17 AS.seek 0ml @09:52:16.53 delay 3 seconds @09:52:19.55 AFV.select :carrierSolution @09:52:19.68 delay 2 seconds @09:52:21.71 AS.reconfigure ASconfig @09:52:22.36 AS.seek 1ml @09:53:20.12 delay 1 second @09:53:21.14 AFV.select :holdingCoil @09:53:21.27 delay 1 second @09:53:22.30 AS.reconfigure ASfast @09:53:22.37 AS.seek 0ml @09:53:49.73 delay 3 seconds @09:53:52.75 AFV.select :carrierSolution @09:53:52.88 delay 2 seconds @09:53:54.91 AS.reconfigure ASconfig @09:53:54.99 AS.seek 1ml @09:54:52.80 delay 1 second @09:54:53.81 AFV.select :holdingCoil @09:54:53.94 delay 1 second @09:54:54.97 AS.reconfigure ASfast @09:54:55.04 AS.seek 0ml @09:55:22.42 delay 3 seconds @09:55:25.44 AFV.select :carrierSolution @09:55:25.57 delay 2 seconds @09:55:27.60 AS.reconfigure ASconfig @09:55:27.68 AS.seek 1ml @09:56:25.48 delay 1 second @09:56:26.50 AFV.select :holdingCoil @09:56:26.63 delay 1 second @09:56:27.66 AS.reconfigure ASfast @09:56:27.73 AS.seek 0ml @09:56:55.09 delay 3 seconds @09:56:58.11 AFV.select :carrierSolution @09:56:58.24 delay 2 seconds @09:57:00.27 AS.reconfigure ASconfig @09:57:00.35 AS.seek 1ml @09:57:58.20 delay 1 second @09:57:59.22 AFV.select :holdingCoil @09:57:59.35 delay 1 second @09:58:00.38 AS.reconfigure ASnorm @09:58:00.45 AS.seek 0ml @10:03:21.71 Session Ended @10:06:30.49 Session Begun @10:06:36.54 -> showlog 100 @10:10:09.58 -> ESP.shutdown @10:10:09.62 AS.coast @10:10:09.65 Asleep / Shutdown @10:10:09.72 SS.coast @10:10:09.79 CC.coast @10:10:09.84 CS.coast @10:10:09.91 PC.coast @10:10:09.97 Forearm.coast @10:10:10.03 Elbow.coast @10:10:10.09 SC.coast @10:10:10.15 SE.coast @10:10:11.02 Gate.power :core,:OFF @10:10:11.12 Gate.power :raw,:OFF @10:10:11.15 Shutdown @10:10:17.60 -> exit @10:10:17.62 Session Ended @14:00:00.06
Powering core bus @14:00:05.01 Gate.power :raw,:ON @14:00:05.68 SPE.coast @14:00:05.75 SPE.configure SPEconfig @14:00:05.84 AS.configure ASconfig @14:00:05.95 SS.configure SSconfig @14:00:06.05 CC.configure CCconfig @14:00:06.15 CS.configure CSconfig @14:00:06.25 PC.configure PCconfig @14:00:06.35 PS.configure PSconfig @14:00:06.48 Forearm.configure LooseForearm @14:00:06.58 Elbow.configure LooseElbow @14:00:06.70 SC.configure SCloose @14:00:06.80 SE.configure SEconfig @14:00:06.85 CH.coast @14:00:06.91 CH.configure CHconfig @14:00:06.94 PH.coast @14:00:07.00 PH.configure PHconfig @14:00:07.04 PCR.off @14:00:07.08 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:07.10 PCR.caltemp= 2.7073,0.0373,0 @14:00:07.13 All dwarf controllers running firmware version 3.75 @14:00:07.25 Valve.reset @14:00:07.42 PCR.off @14:00:07.45 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:07.48 PCR.caltemp= 2.7073,0.0373,0 @14:00:07.71 PSV.dial :air @14:00:07.74 Intake.select :close @14:00:07.77 Exhaust.select :close @14:00:07.80 CTV.dial :air @14:00:07.97 CSV.dial :waste1 @14:00:08.00 PS.reconfigure PSconfig @14:00:08.09 PS.home.seek 0ml @14:00:08.17 SSV.dial :spit,avoiding: :exhaust @14:00:08.19
Forearm.reconfigure LooseForearm @14:00:08.32 Forearm.home.seek :retract @14:00:08.44 CS.reconfigure CSfast @14:00:08.47 SS.reconfigure SSconfig @14:00:08.56 CS.home.seek 0ml @14:00:08.58 SS.home.seek 0ml @14:00:09.08 Hand.close @14:00:09.15
Elbow.jog 1500 @14:00:09.60 Elbow.reconfigure LooseElbow @14:00:09.64 Elbow.home.seek :clear @14:00:14.26 SE.reconfigure SEconfig @14:00:14.31 SE.home.seek :down @14:00:15.05 SC.reconfigure SCsloppy @14:00:15.10 SC.home.seek :initial @14:00:17.17 PS.coast @14:00:19.07
Forearm.seek :retract @14:00:19.15 Elbow.seek :hammer @14:00:20.47 Forearm.reconfigure TightForearm @14:00:20.52 Forearm.seek 5 + 350 counts @14:00:21.59 Forearm.seek :retract @14:00:22.75 CC.reconfigure CCconfig @14:00:22.78 PC.reconfigure PCconfig @14:00:22.87
CC.home.seek :open @14:00:22.90 PC.home.seek :open @14:00:26.40
Phase [phasewithdil] @14:00:26.42 GoodNews.email "At 14:00:26 began in tube 2: phasewithdil\n/home/esp/esp2/mission/18tundeployv2dil.rb:118\n{1 => 18, 2 => 4}\nCan@14:00:26, 13.9C, 69% humidity, 14.3psia, 13.756V, 0.790A, 0.790A avg, 10.86W",Subject: "Started 18tundeployv2dil phase phasewithdil" @14:00:26.45CEST06-Oct-18
==================> STARTED Superphasewdil (phase #3) <=== @14:00:26.47 Starting initialPurge @14:00:26.49 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:00:26.56 Hand.open @14:00:27.34 Elbow.reconfigure TightElbow @14:00:27.39 Elbow.seek FlushPuck::Garage @14:00:29.27 Elbow.reconfigure LooseElbow @14:00:29.38 Forearm.reconfigure LooseForearm @14:00:29.43 Forearm.seek :garage @14:00:30.47 Hand.close @14:00:31.23 Forearm.seek :retract @14:00:32.27 Elbow.reconfigure TightElbow @14:00:32.32 Elbow.seek CC @14:00:35.28 Elbow.reconfigure LooseElbow @14:00:35.39 Forearm.reconfigure TightForearm @14:00:35.44 Forearm.seek CC @14:00:36.75 CC.seek :closed @14:00:40.98 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.91 @14:00:41.07 Hand.open @14:00:41.79 Forearm.reconfigure LooseForearm @14:00:41.84 Forearm.seek :retract @14:00:42.83 Flushing the Collection Valve::Manifold @14:00:42.97 CSV.dial CSR @14:00:44.36 Pulling 5ml of CSR.flush @14:00:44.37 CSR.select :flush @14:00:44.50 CS.reconfigure CSconfig @14:00:44.54 CS.seek 5ml @14:00:58.25 delay 5 seconds @14:01:03.27 CSR.select :air @14:01:03.44 CS.reconfigure CSfast @14:01:03.49 CS.seek 7.5ml @14:01:10.24 CSV.dial :waste @14:01:11.57 CS.reconfigure CSconfig @14:01:11.62 CS.seek 0ml @14:01:31.79 Purging external Intake @14:01:31.92 CSV.dial :flush @14:01:32.40 CS.reconfigure CSfast @14:01:32.45 CS.seek 10ml @14:01:55.85 delay 5 seconds @14:02:00.88 CSV.dial CBV @14:02:00.92
CBV.dial CSV @14:02:01.68 CTV.dial :intake @14:02:02.45 Intake.select :open @14:02:02.58 CS.seek 0ml @14:02:26.87 Intake.select :close @14:02:26.99 CTV.dial :air @14:02:27.74 Flushing upper manifold @14:02:27.87 CSV.dial :flush @14:02:28.64 CS.seek 5ml @14:02:41.32 delay 5 seconds @14:02:46.34 CSV.dial :air @14:02:46.88 CS.seek 7ml @14:02:52.51 CSV.dial CBV @14:02:52.55
CBV.dial CSV @14:02:53.60 CTV.dial :air @14:02:53.62 CS.seek 0ml @14:03:10.16 delay 7 seconds @14:03:17.20 CS.seek 3ml @14:03:24.97 CSV.dial :waste @14:03:25.47 CS.seek 0ml @14:03:33.38 CSV.dial :flush @14:03:33.84 CS.seek 3ml @14:03:41.57 delay 5 seconds @14:03:46.59 CSV.dial :air @14:03:47.14 CS.seek 5ml @14:03:52.68 CSV.dial :air @14:03:52.71 CS.seek 0ml @14:04:04.79 delay 7 seconds @14:04:11.82 CS.seek 3ml @14:04:19.59 CSV.dial :waste @14:04:20.36 CS.seek 0ml @14:04:28.07 Garaging FlushPuck in Collection Clamp @14:04:28.15 Elbow.reconfigure TightElbow @14:04:28.20 Elbow.seek CC @14:04:28.44 Elbow.reconfigure LooseElbow @14:04:28.55 Forearm.seek CC @14:04:29.53 Hand.close @14:04:30.21 CC.seek :open @14:04:34.54 Forearm.seek :retract @14:04:35.56 Elbow.reconfigure TightElbow @14:04:35.61 Elbow.seek :garage @14:04:38.71 Elbow.reconfigure LooseElbow @14:04:38.81 Forearm.reconfigure TightForearm @14:04:38.86 Forearm.seek :garage @14:04:40.01 Hand.open @14:04:40.66 Forearm.reconfigure LooseForearm @14:04:40.70 Forearm.seek :retract @14:04:41.80 Starting mfb @14:04:41.81 pcrBAC sampling at most 1500ml, wcr at most 1500ml @14:04:41.84 BACfans.email "Started mfb in tube 2 at 14:04:41CEST06-Oct-18\n{1 => 18, 2 => 4}\nCan@14:00:26, 13.9C, 69% humidity, 14.3psia, 13.756V, 0.790A, 0.790A avg, 10.86W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @14:04:41.95
Loading sh1 puck from tube 2 into Collection Clamp @14:04:42.09 Readying the MFB @14:04:42.10 Homing MFB Syringe @14:04:42.11 AFV.select :holdingCoil @14:04:42.35 ATV.dial :air2 @14:04:42.47
SC.reconfigure SCloose @14:04:42.71 Elbow.reconfigure TightElbow @14:04:42.76 Elbow.seek 2 @14:04:43.77 AS.reconfigure ASconfig @14:04:43.82 AS.home.seek :home @14:04:44.84
Forearm.reconfigure TightForearm @14:04:44.92 Forearm.seek 2 + 700 counts @14:04:45.96 SE.reconfigure SEpick @14:04:46.05 SE.seek beamBreak @14:04:48.90 SE.configure SEconfig @14:04:49.16 Elbow.seek 2 @14:04:49.46 AFV.select :carrierSolution @14:04:49.62
Forearm.seek 2 + 300 counts @14:04:49.68 delay 2 seconds @14:04:49.89
Forearm.reconfigure LooseForearm @14:04:49.94 Hand.close @14:04:50.61 SE.seek :down @14:04:50.87
Forearm.seek :retract @14:04:51.71 AS.seek 1ml @14:04:51.83
Elbow.seek CC @14:04:53.15 SE.coast @14:04:53.47
Elbow.reconfigure LooseElbow @14:04:53.59 Forearm.reconfigure TightForearm @14:04:53.68 Forearm.seek CC @14:04:55.02 CC.seek :closed @14:04:59.27 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 1.95 @14:04:59.29 Hand.open @14:05:00.03 Forearm.reconfigure LooseForearm @14:05:00.08 Forearm.seek :retract @14:05:01.10 SC.coast @14:05:01.14 collectSample 1500ml w/bubble point of 55psi @14:05:01.16 Priming 7ml @14:05:01.17 Intake.select :close @14:05:01.18 Exhaust.select :close @14:05:01.19 SS.hold @14:05:01.43 Calibrating pressure and temperature sensors @14:05:01.45 CTV.dial :air @14:05:01.46 SSV.dial :spit,avoiding: :exhaust @14:05:01.49 SS.reconfigure SSfast @14:05:01.54 SS.seek 5ml @14:05:04.13 SSV.dial CBV @14:05:04.16
CBV.dial SSV @14:05:05.54 delay 3.8 seconds @14:05:09.49 SSV.dial :spit @14:05:10.86 SS.seek 0ml @14:05:13.45 SSV.dial CBV @14:05:14.80 CTV.dial :intake @14:05:15.59 SSV.dial CBV @14:05:15.62
CBV.dial SSV @14:05:15.64 Allowing 40s for 7.0ml of remaining 7.0ml @14:05:15.65 Intake.select :open @14:05:17.82 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:05:17.84 SS.reconfigure SSinhale.with maxSpeed: 150 @14:05:17.89 SS.seek 7ml @14:05:20.99 Vacuum too high -- waiting up to 40s @14:05:22.60 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:05:22.62 SS.reconfigure SSinhale.with maxSpeed: 135 @14:05:22.67 SS.seek 7ml @14:05:25.25 Vacuum too high -- waiting up to 40s @14:05:26.90 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:05:26.92 SS.reconfigure SSinhale.with maxSpeed: 122 @14:05:26.97 SS.seek 7ml @14:05:28.98 Stroke completed -- waiting for pressure to equalize ... @14:05:31.61 Intake.select :close @14:05:31.77 SSV.dial :exhaust @14:05:32.57 Exhaust.select :open @14:05:32.68 SS.reconfigure SSexhale @14:05:32.73 SS.seek 0ml @14:05:36.61 Exhaust.select :close @14:05:36.72 Sampling 1500.0ml @14:05:36.73 Intake.select :close @14:05:36.74 Exhaust.select :close @14:05:36.76 SS.reconfigure SSconfig @14:05:36.80 SS.hold @14:05:37.03 CTV.dial :intake @14:05:37.04 SSV.dial CBV @14:05:37.07
CBV.dial SSV @14:05:37.90 Allowing 130s for 25.0ml of remaining 1500.0ml @14:05:37.91 Intake.select :open @14:05:40.10 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:05:40.11 SS.reconfigure SSinhale.with maxSpeed: 122 @14:05:40.16 SS.seek 25ml @14:05:43.71 Vacuum too high -- waiting up to 40s @14:05:44.62 delay 1 second @14:05:45.09
Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:05:45.11 SS.reconfigure SSinhale.with maxSpeed: 110 @14:05:45.16 SS.seek 25ml @14:05:45.64 AFV.select :holdingCoil @14:05:45.75 delay 1 second @14:05:46.78 ATV.dial :waste @14:05:47.62
Vacuum too high -- waiting up to 40s @14:05:47.92 AS.reconfigure ASnorm @14:05:47.97 AS.home.seek :empty @14:05:49.04
SS.reconfigure SSinhale.with maxSpeed: 99 @14:05:49.09 SS.seek 25ml @14:05:51.67 Vacuum too high -- waiting up to 40s @14:05:53.02 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:05:53.04 SS.reconfigure SSinhale.with maxSpeed: 89 @14:05:53.09 SS.seek 25ml @14:05:55.62 Vacuum too high -- waiting up to 40s @14:05:57.50 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:05:57.52 SS.reconfigure SSinhale.with maxSpeed: 80 @14:05:57.57 SS.seek 25ml @14:06:01.09 Vacuum too high -- waiting up to 40s @14:06:02.43 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:06:02.45 SS.reconfigure SSinhale.with maxSpeed: 72 @14:06:02.50 SS.seek 25ml @14:06:05.80 Vacuum too high -- waiting up to 40s @14:06:06.91 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:06:06.93 SS.reconfigure SSinhale.with maxSpeed: 65 @14:06:06.98 SS.seek 25ml @14:06:10.74 Vacuum too high -- waiting up to 40s @14:06:12.10 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:06:12.11 SS.reconfigure SSinhale.with maxSpeed: 59 @14:06:12.16 SS.seek 25ml @14:06:17.43 Vacuum too high -- waiting up to 40s @14:06:18.52 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:06:18.54 SS.reconfigure SSinhale.with maxSpeed: 53 @14:06:18.59 SS.seek 25ml @14:06:21.01 Stroke completed -- waiting for pressure to equalize ... @14:06:23.62 Intake.select :close @14:06:23.78 SSV.dial :exhaust @14:06:24.57 Exhaust.select :open @14:06:24.68 SS.reconfigure SSexhale @14:06:24.73 SS.seek 0ml @14:06:33.68 Exhaust.select :close @14:06:33.80 SSV.dial CBV @14:06:33.83
CBV.dial SSV @14:06:34.63 Allowing 130s for 25.0ml of remaining 1475.0ml @14:06:34.64 Intake.select :open @14:06:36.81 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:06:36.83 SS.reconfigure SSinhale.with maxSpeed: 53 @14:06:36.88 SS.seek 25ml @14:06:40.53 ATV.dial :waste @14:06:40.55 ARV.dial between carrier and air1 @14:06:40.65 ASV.dial :waste1 @14:06:46.30
Vacuum too high -- waiting up to 40s @14:06:47.13 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:06:47.15 SS.reconfigure SSinhale.with maxSpeed: 48 @14:06:47.20 SS.seek 25ml @14:07:14.42 Stroke completed -- waiting for pressure to equalize ... @14:07:17.61 Intake.select :close @14:07:18.28 SSV.dial :exhaust @14:07:19.10 Exhaust.select :open @14:07:19.21 SS.reconfigure SSexhale @14:07:19.26 SS.seek 0ml @14:07:28.18 Exhaust.select :close @14:07:28.30 SSV.dial CBV @14:07:28.33
CBV.dial SSV @14:07:29.16 Allowing 130s for 25.0ml of remaining 1450.0ml @14:07:29.17 Intake.select :open @14:07:31.35 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:07:31.36 SS.reconfigure SSinhale.with maxSpeed: 48 @14:07:31.41 SS.seek 25ml @14:08:05.53 Vacuum too high -- waiting up to 40s @14:08:06.36 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:08:06.38 SS.reconfigure SSinhale.with maxSpeed: 43 @14:08:06.43 SS.seek 25ml @14:08:10.43 Stroke completed -- waiting for pressure to equalize ... @14:08:12.44 Intake.select :close @14:08:12.60 SSV.dial :exhaust @14:08:13.40 Exhaust.select :open @14:08:13.51 SS.reconfigure SSexhale @14:08:13.56 SS.seek 0ml @14:08:22.62 Exhaust.select :close @14:08:22.74 SSV.dial CBV @14:08:22.77
CBV.dial SSV @14:08:23.60 Allowing 130s for 25.0ml of remaining 1425.0ml @14:08:23.61 Intake.select :open @14:08:25.78 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:08:25.80 SS.reconfigure SSinhale.with maxSpeed: 43 @14:08:25.85 SS.seek 25ml @14:09:07.01 Stroke completed -- waiting for pressure to equalize ... @14:09:09.61 Intake.select :close @14:09:09.76 SSV.dial :exhaust @14:09:10.54 Exhaust.select :open @14:09:10.66 SS.reconfigure SSexhale @14:09:10.71 SS.seek 0ml @14:09:19.74 Exhaust.select :close @14:09:19.86 SSV.dial CBV @14:09:19.89
CBV.dial SSV @14:09:20.69 Allowing 130s for 25.0ml of remaining 1400.0ml @14:09:20.70 Intake.select :open @14:09:22.87 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:09:22.89 SS.reconfigure SSinhale.with maxSpeed: 43 @14:09:22.94 SS.seek 25ml @14:10:04.24 Stroke completed -- waiting for pressure to equalize ... @14:10:06.81 Intake.select :close @14:10:06.96 SSV.dial :exhaust @14:10:07.76 Exhaust.select :open @14:10:07.87 SS.reconfigure SSexhale @14:10:07.92 SS.seek 0ml @14:10:16.89 Exhaust.select :close @14:10:17.01 SSV.dial CBV @14:10:17.04
CBV.dial SSV @14:10:17.86 Allowing 130s for 25.0ml of remaining 1375.0ml @14:10:17.87 Intake.select :open @14:10:20.04 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:10:20.06 SS.reconfigure SSinhale.with maxSpeed: 43 @14:10:20.11 SS.seek 25ml @14:11:01.76 Stroke completed -- waiting for pressure to equalize ... @14:11:04.32 Intake.select :close @14:11:04.47 SSV.dial :exhaust @14:11:05.27 Exhaust.select :open @14:11:05.86 SS.reconfigure SSexhale @14:11:05.95 SS.seek 0ml @14:11:15.00 Exhaust.select :close @14:11:15.12 SSV.dial CBV @14:11:15.15
CBV.dial SSV @14:11:15.96 Allowing 130s for 25.0ml of remaining 1350.0ml @14:11:15.97 Intake.select :open @14:11:18.14 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:11:18.16 SS.reconfigure SSinhale.with maxSpeed: 43 @14:11:18.21 SS.seek 25ml @14:11:59.31 Stroke completed -- waiting for pressure to equalize ... @14:12:01.93 Intake.select :close @14:12:02.09 SSV.dial :exhaust @14:12:02.88 Exhaust.select :open @14:12:02.99 SS.reconfigure SSexhale @14:12:03.04 SS.seek 0ml @14:12:11.92 Exhaust.select :close @14:12:12.04 SSV.dial CBV @14:12:12.07
CBV.dial SSV @14:12:12.90 Allowing 130s for 25.0ml of remaining 1325.0ml @14:12:12.91 Intake.select :open @14:12:15.09 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:12:15.10 SS.reconfigure SSinhale.with maxSpeed: 43 @14:12:15.15 SS.seek 25ml @14:12:56.57 Stroke completed -- waiting for pressure to equalize ... @14:12:59.16 Intake.select :close @14:12:59.32 SSV.dial :exhaust @14:13:00.11 Exhaust.select :open @14:13:00.22 SS.reconfigure SSexhale @14:13:00.27 SS.seek 0ml @14:13:09.49 Exhaust.select :close @14:13:09.61 SSV.dial CBV @14:13:09.64
CBV.dial SSV @14:13:10.46 Allowing 130s for 25.0ml of remaining 1300.0ml @14:13:10.47 Intake.select :open @14:13:12.64 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:13:12.66 SS.reconfigure SSinhale.with maxSpeed: 43 @14:13:12.71 SS.seek 25ml @14:13:54.14 Stroke completed -- waiting for pressure to equalize ... @14:13:56.73 Intake.select :close @14:13:56.89 SSV.dial :exhaust @14:13:57.67 Exhaust.select :open @14:13:57.79 SS.reconfigure SSexhale @14:13:57.84 SS.seek 0ml @14:14:06.99 Exhaust.select :close @14:14:07.12 SSV.dial CBV @14:14:07.18
CBV.dial SSV @14:14:07.99 Allowing 130s for 25.0ml of remaining 1275.0ml @14:14:08.00 Intake.select :open @14:14:10.17 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:14:10.19 SS.reconfigure SSinhale.with maxSpeed: 43 @14:14:10.24 SS.seek 25ml @14:14:51.64 Stroke completed -- waiting for pressure to equalize ... @14:14:54.23 Intake.select :close @14:14:54.38 SSV.dial :exhaust @14:14:55.18 Exhaust.select :open @14:14:55.29 SS.reconfigure SSexhale @14:14:55.35 SS.seek 0ml @14:15:04.37 Exhaust.select :close @14:15:04.49 SSV.dial CBV @14:15:04.52
CBV.dial SSV @14:15:05.33 Allowing 130s for 25.0ml of remaining 1250.0ml @14:15:05.34 Intake.select :open @14:15:07.54 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:15:07.56 SS.reconfigure SSinhale.with maxSpeed: 43 @14:15:07.61 SS.seek 25ml @14:15:49.65 Stroke completed -- waiting for pressure to equalize ... @14:15:52.24 Intake.select :close @14:15:52.40 SSV.dial :exhaust @14:15:53.19 Exhaust.select :open @14:15:53.30 SS.reconfigure SSexhale @14:15:53.35 SS.seek 0ml @14:16:02.59 Exhaust.select :close @14:16:02.71 SSV.dial CBV @14:16:02.74
CBV.dial SSV @14:16:03.57 Allowing 130s for 25.0ml of remaining 1225.0ml @14:16:03.58 Intake.select :open @14:16:05.75 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:16:05.77 SS.reconfigure SSinhale.with maxSpeed: 43 @14:16:05.82 SS.seek 25ml @14:16:46.65 Stroke completed -- waiting for pressure to equalize ... @14:16:49.25 Intake.select :close @14:16:49.41 SSV.dial :exhaust @14:16:50.19 Exhaust.select :open @14:16:50.30 SS.reconfigure SSexhale @14:16:50.35 SS.seek 0ml @14:16:59.37 Exhaust.select :close @14:16:59.49 SSV.dial CBV @14:16:59.52
CBV.dial SSV @14:17:00.32 Allowing 130s for 25.0ml of remaining 1200.0ml @14:17:00.33 Intake.select :open @14:17:02.50 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:17:02.52 SS.reconfigure SSinhale.with maxSpeed: 43 @14:17:02.57 SS.seek 25ml @14:17:43.90 Stroke completed -- waiting for pressure to equalize ... @14:17:46.50 Intake.select :close @14:17:46.65 SSV.dial :exhaust @14:17:47.43 Exhaust.select :open @14:17:47.55 SS.reconfigure SSexhale @14:17:47.60 SS.seek 0ml @14:17:56.34 Exhaust.select :close @14:17:56.46 SSV.dial CBV @14:17:56.49
CBV.dial SSV @14:17:57.32 Allowing 130s for 25.0ml of remaining 1175.0ml @14:17:57.33 Intake.select :open @14:17:59.50 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:17:59.52 SS.reconfigure SSinhale.with maxSpeed: 43 @14:17:59.57 SS.seek 25ml @14:18:41.29 Stroke completed -- waiting for pressure to equalize ... @14:18:44.48 Intake.select :close @14:18:44.63 SSV.dial :exhaust @14:18:45.42 Exhaust.select :open @14:18:45.54 SS.reconfigure SSexhale @14:18:45.59 SS.seek 0ml @14:18:54.71 Exhaust.select :close @14:18:54.83 SSV.dial CBV @14:18:54.86
CBV.dial SSV @14:18:55.68 Allowing 130s for 25.0ml of remaining 1150.0ml @14:18:55.69 Intake.select :open @14:18:57.86 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:18:57.88 SS.reconfigure SSinhale.with maxSpeed: 43 @14:18:57.93 SS.seek 25ml @14:19:39.40 Stroke completed -- waiting for pressure to equalize ... @14:19:42.42 Intake.select :close @14:19:42.67 SSV.dial :exhaust @14:19:43.46 Exhaust.select :open @14:19:43.57 SS.reconfigure SSexhale @14:19:43.62 SS.seek 0ml @14:19:52.37 Exhaust.select :close @14:19:52.49 SSV.dial CBV @14:19:52.52
CBV.dial SSV @14:19:53.35 Allowing 130s for 25.0ml of remaining 1125.0ml @14:19:53.36 Intake.select :open @14:19:55.53 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:19:55.55 SS.reconfigure SSinhale.with maxSpeed: 43 @14:19:55.60 SS.seek 25ml @14:20:37.28 Stroke completed -- waiting for pressure to equalize ... @14:20:40.51 Intake.select :close @14:20:40.67 SSV.dial :exhaust @14:20:41.46 Exhaust.select :open @14:20:41.57 SS.reconfigure SSexhale @14:20:41.62 SS.seek 0ml @14:20:50.37 Exhaust.select :close @14:20:50.49 SSV.dial CBV @14:20:50.52
CBV.dial SSV @14:20:51.33 Allowing 130s for 25.0ml of remaining 1100.0ml @14:20:51.34 Intake.select :open @14:20:53.52 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:20:53.53 SS.reconfigure SSinhale.with maxSpeed: 43 @14:20:53.59 SS.seek 25ml @14:21:35.23 Stroke completed -- waiting for pressure to equalize ... @14:21:38.45 Intake.select :close @14:21:38.61 SSV.dial :exhaust @14:21:39.40 Exhaust.select :open @14:21:39.51 SS.reconfigure SSexhale @14:21:39.56 SS.seek 0ml @14:21:48.54 Exhaust.select :close @14:21:48.67 SSV.dial CBV @14:21:48.69
CBV.dial SSV @14:21:49.50 Allowing 130s for 25.0ml of remaining 1075.0ml @14:21:49.51 Intake.select :open @14:21:51.68 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:21:51.70 SS.reconfigure SSinhale.with maxSpeed: 43 @14:21:51.75 SS.seek 25ml @14:22:33.06 Stroke completed -- waiting for pressure to equalize ... @14:22:36.25 Intake.select :close @14:22:36.40 SSV.dial :exhaust @14:22:37.18 Exhaust.select :open @14:22:37.30 SS.reconfigure SSexhale @14:22:37.35 SS.seek 0ml @14:22:46.12 Exhaust.select :close @14:22:46.24 SSV.dial CBV @14:22:46.27
CBV.dial SSV @14:22:47.08 Allowing 130s for 25.0ml of remaining 1050.0ml @14:22:47.09 Intake.select :open @14:22:49.27 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:22:49.28 SS.reconfigure SSinhale.with maxSpeed: 43 @14:22:49.33 SS.seek 25ml @14:23:21.48 Vacuum too high -- waiting up to 40s @14:23:22.30 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:23:22.32 SS.reconfigure SSinhale.with maxSpeed: 39 @14:23:22.37 SS.seek 25ml @14:23:32.98 Stroke completed -- waiting for pressure to equalize ... @14:23:36.08 Intake.select :close @14:23:36.24 SSV.dial :exhaust @14:23:37.04 Exhaust.select :open @14:23:37.15 SS.reconfigure SSexhale @14:23:37.20 SS.seek 0ml @14:23:46.37 Exhaust.select :close @14:23:46.49 SSV.dial CBV @14:23:46.52
CBV.dial SSV @14:23:47.33 Allowing 130s for 25.0ml of remaining 1025.0ml @14:23:47.34 Intake.select :open @14:23:49.51 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:23:49.53 SS.reconfigure SSinhale.with maxSpeed: 39 @14:23:49.58 SS.seek 25ml @14:24:34.67 Stroke completed -- waiting for pressure to equalize ... @14:24:37.27 Intake.select :close @14:24:37.43 SSV.dial :exhaust @14:24:38.22 Exhaust.select :open @14:24:38.33 SS.reconfigure SSexhale @14:24:38.38 SS.seek 0ml @14:24:47.12 Exhaust.select :close @14:24:47.24 SSV.dial CBV @14:24:47.27
CBV.dial SSV @14:24:48.08 Allowing 130s for 25.0ml of remaining 1000.0ml @14:24:48.09 Intake.select :open @14:24:50.26 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:24:50.28 SS.reconfigure SSinhale.with maxSpeed: 39 @14:24:50.33 SS.seek 25ml @14:25:36.09 Stroke completed -- waiting for pressure to equalize ... @14:25:38.68 Intake.select :close @14:25:38.83 SSV.dial :exhaust @14:25:39.62 Exhaust.select :open @14:25:39.74 SS.reconfigure SSexhale @14:25:39.79 SS.seek 0ml @14:25:48.85 Exhaust.select :close @14:25:48.97 SSV.dial CBV @14:25:49.00
CBV.dial SSV @14:25:49.82 Allowing 130s for 25.0ml of remaining 975.0ml @14:25:49.83 Intake.select :open @14:25:52.00 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:25:52.02 SS.reconfigure SSinhale.with maxSpeed: 39 @14:25:52.07 SS.seek 25ml @14:26:37.70 Stroke completed -- waiting for pressure to equalize ... @14:26:40.27 Intake.select :close @14:26:40.43 SSV.dial :exhaust @14:26:41.21 Exhaust.select :open @14:26:41.33 SS.reconfigure SSexhale @14:26:41.38 SS.seek 0ml @14:26:50.49 Exhaust.select :close @14:26:50.61 SSV.dial CBV @14:26:50.64
CBV.dial SSV @14:26:51.44 Allowing 130s for 25.0ml of remaining 950.0ml @14:26:51.45 Intake.select :open @14:26:53.62 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:26:53.64 SS.reconfigure SSinhale.with maxSpeed: 39 @14:26:53.69 SS.seek 25ml @14:27:39.20 Stroke completed -- waiting for pressure to equalize ... @14:27:42.40 Intake.select :close @14:27:42.55 SSV.dial :exhaust @14:27:43.35 Exhaust.select :open @14:27:43.84 SS.reconfigure SSexhale @14:27:43.97 SS.seek 0ml @14:27:53.03 Exhaust.select :close @14:27:53.15 SSV.dial CBV @14:27:53.18
CBV.dial SSV @14:27:54.00 Allowing 130s for 25.0ml of remaining 925.0ml @14:27:54.01 Intake.select :open @14:27:56.18 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:27:56.20 SS.reconfigure SSinhale.with maxSpeed: 39 @14:27:56.25 SS.seek 25ml @14:28:41.65 Stroke completed -- waiting for pressure to equalize ... @14:28:44.89 Intake.select :close @14:28:45.05 SSV.dial :exhaust @14:28:45.83 Exhaust.select :open @14:28:45.94 SS.reconfigure SSexhale @14:28:45.99 SS.seek 0ml @14:28:54.76 Exhaust.select :close @14:28:54.88 SSV.dial CBV @14:28:54.91
CBV.dial SSV @14:28:55.72 Allowing 130s for 25.0ml of remaining 900.0ml @14:28:55.73 Intake.select :open @14:28:57.90 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:28:57.92 SS.reconfigure SSinhale.with maxSpeed: 39 @14:28:57.97 SS.seek 25ml @14:29:43.93 Stroke completed -- waiting for pressure to equalize ... @14:29:47.15 Intake.select :close @14:29:47.31 SSV.dial :exhaust @14:29:48.10 Exhaust.select :open @14:29:48.21 SS.reconfigure SSexhale @14:29:48.26 SS.seek 0ml @14:29:57.42 Exhaust.select :close @14:29:57.54 SSV.dial CBV @14:29:57.57
CBV.dial SSV @14:29:58.38 Allowing 130s for 25.0ml of remaining 875.0ml @14:29:58.39 Intake.select :open @14:30:00.56 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:30:00.58 SS.reconfigure SSinhale.with maxSpeed: 39 @14:30:00.63 SS.seek 25ml @14:30:46.03 Stroke completed -- waiting for pressure to equalize ... @14:30:49.22 Intake.select :close @14:30:49.38 SSV.dial :exhaust @14:30:50.17 Exhaust.select :open @14:30:50.29 SS.reconfigure SSexhale @14:30:50.34 SS.seek 0ml @14:30:59.42 Exhaust.select :close @14:30:59.54 SSV.dial CBV @14:30:59.57
CBV.dial SSV @14:31:00.40 Allowing 130s for 25.0ml of remaining 850.0ml @14:31:00.41 Intake.select :open @14:31:02.58 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:31:02.60 SS.reconfigure SSinhale.with maxSpeed: 39 @14:31:02.65 SS.seek 25ml @14:31:48.09 Stroke completed -- waiting for pressure to equalize ... @14:31:51.29 Intake.select :close @14:31:51.44 SSV.dial :exhaust @14:31:52.22 Exhaust.select :open @14:31:52.33 SS.reconfigure SSexhale @14:31:52.39 SS.seek 0ml @14:32:01.60 Exhaust.select :close @14:32:02.25 SSV.dial CBV @14:32:02.28
CBV.dial SSV @14:32:03.08 Allowing 130s for 25.0ml of remaining 825.0ml @14:32:03.09 Intake.select :open @14:32:05.27 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:32:05.29 SS.reconfigure SSinhale.with maxSpeed: 39 @14:32:05.34 SS.seek 25ml @14:32:50.68 Stroke completed -- waiting for pressure to equalize ... @14:32:54.56 Intake.select :close @14:32:54.72 SSV.dial :exhaust @14:32:55.50 Exhaust.select :open @14:32:55.61 SS.reconfigure SSexhale @14:32:55.66 SS.seek 0ml @14:33:04.74 Exhaust.select :close @14:33:04.86 SSV.dial CBV @14:33:04.89
CBV.dial SSV @14:33:05.71 Allowing 130s for 25.0ml of remaining 800.0ml @14:33:05.72 Intake.select :open @14:33:07.90 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:33:07.91 SS.reconfigure SSinhale.with maxSpeed: 39 @14:33:07.97 SS.seek 25ml @14:33:51.53 Vacuum too high -- waiting up to 40s @14:33:52.62 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:33:52.64 SS.reconfigure SSinhale.with maxSpeed: 35 @14:33:52.69 SS.seek 25ml @14:33:54.87 Stroke completed -- waiting for pressure to equalize ... @14:33:57.48 Intake.select :close @14:33:57.63 SSV.dial :exhaust @14:33:58.43 Exhaust.select :open @14:33:58.54 SS.reconfigure SSexhale @14:33:58.59 SS.seek 0ml @14:34:07.76 Exhaust.select :close @14:34:07.88 SSV.dial CBV @14:34:07.91
CBV.dial SSV @14:34:08.72 Allowing 130s for 25.0ml of remaining 775.0ml @14:34:08.73 Intake.select :open @14:34:10.91 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:34:10.92 SS.reconfigure SSinhale.with maxSpeed: 35 @14:34:10.98 SS.seek 25ml @14:35:01.40 Stroke completed -- waiting for pressure to equalize ... @14:35:04.61 Intake.select :close @14:35:04.76 SSV.dial :exhaust @14:35:05.55 Exhaust.select :open @14:35:05.66 SS.reconfigure SSexhale @14:35:05.71 SS.seek 0ml @14:35:14.65 Exhaust.select :close @14:35:14.77 SSV.dial CBV @14:35:14.80
CBV.dial SSV @14:35:15.60 Allowing 130s for 25.0ml of remaining 750.0ml @14:35:15.61 Intake.select :open @14:35:17.78 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:35:17.80 SS.reconfigure SSinhale.with maxSpeed: 35 @14:35:17.85 SS.seek 25ml @14:36:08.59 Stroke completed -- waiting for pressure to equalize ... @14:36:12.31 Intake.select :close @14:36:12.47 SSV.dial :exhaust @14:36:13.25 Exhaust.select :open @14:36:13.36 SS.reconfigure SSexhale @14:36:13.41 SS.seek 0ml @14:36:22.49 Exhaust.select :close @14:36:22.61 SSV.dial CBV @14:36:22.64
CBV.dial SSV @14:36:23.46 Allowing 130s for 25.0ml of remaining 725.0ml @14:36:23.47 Intake.select :open @14:36:25.65 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:36:25.66 SS.reconfigure SSinhale.with maxSpeed: 35 @14:36:25.71 SS.seek 25ml @14:37:16.15 Stroke completed -- waiting for pressure to equalize ... @14:37:19.39 Intake.select :close @14:37:19.55 SSV.dial :exhaust @14:37:20.35 Exhaust.select :open @14:37:20.46 SS.reconfigure SSexhale @14:37:20.51 SS.seek 0ml @14:37:29.38 Exhaust.select :close @14:37:29.50 SSV.dial CBV @14:37:29.53
CBV.dial SSV @14:37:30.33 Allowing 130s for 25.0ml of remaining 700.0ml @14:37:30.34 Intake.select :open @14:37:32.52 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:37:32.53 SS.reconfigure SSinhale.with maxSpeed: 35 @14:37:32.58 SS.seek 25ml @14:38:23.32 Stroke completed -- waiting for pressure to equalize ... @14:38:26.53 Intake.select :close @14:38:26.69 SSV.dial :exhaust @14:38:27.49 Exhaust.select :open @14:38:27.60 SS.reconfigure SSexhale @14:38:27.65 SS.seek 0ml @14:38:36.46 Exhaust.select :close @14:38:36.58 SSV.dial CBV @14:38:36.61
CBV.dial SSV @14:38:37.43 Allowing 130s for 25.0ml of remaining 675.0ml @14:38:37.44 Intake.select :open @14:38:39.62 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:38:39.63 SS.reconfigure SSinhale.with maxSpeed: 35 @14:38:39.68 SS.seek 25ml @14:39:30.38 Stroke completed -- waiting for pressure to equalize ... @14:39:34.20 Intake.select :close @14:39:34.36 SSV.dial :exhaust @14:39:35.14 Exhaust.select :open @14:39:35.25 SS.reconfigure SSexhale @14:39:35.30 SS.seek 0ml @14:39:44.15 Exhaust.select :close @14:39:44.27 SSV.dial CBV @14:39:44.30
CBV.dial SSV @14:39:45.11 Allowing 130s for 25.0ml of remaining 650.0ml @14:39:45.12 Intake.select :open @14:39:47.29 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:39:47.31 SS.reconfigure SSinhale.with maxSpeed: 35 @14:39:47.36 SS.seek 25ml @14:40:38.10 Stroke completed -- waiting for pressure to equalize ... @14:40:42.42 Intake.select :close @14:40:42.58 SSV.dial :exhaust @14:40:43.39 Exhaust.select :open @14:40:43.50 SS.reconfigure SSexhale @14:40:43.55 SS.seek 0ml @14:40:52.31 Exhaust.select :close @14:40:52.43 SSV.dial CBV @14:40:52.46
CBV.dial SSV @14:40:53.27 Allowing 130s for 25.0ml of remaining 625.0ml @14:40:53.28 Intake.select :open @14:40:55.47 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:40:55.48 SS.reconfigure SSinhale.with maxSpeed: 35 @14:40:55.53 SS.seek 25ml @14:41:46.31 Stroke completed -- waiting for pressure to equalize ... @14:41:50.18 Intake.select :close @14:41:50.34 SSV.dial :exhaust @14:41:51.13 Exhaust.select :open @14:41:51.24 SS.reconfigure SSexhale @14:41:51.29 SS.seek 0ml @14:42:00.17 Exhaust.select :close @14:42:00.29 SSV.dial CBV @14:42:00.32
CBV.dial SSV @14:42:01.14 Allowing 130s for 25.0ml of remaining 600.0ml @14:42:01.15 Intake.select :open @14:42:03.32 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:42:03.34 SS.reconfigure SSinhale.with maxSpeed: 35 @14:42:03.39 SS.seek 25ml @14:42:54.09 Stroke completed -- waiting for pressure to equalize ... @14:42:57.95 Intake.select :close @14:42:58.11 SSV.dial :exhaust @14:42:58.89 Exhaust.select :open @14:42:59.00 SS.reconfigure SSexhale @14:42:59.05 SS.seek 0ml @14:43:07.90 Exhaust.select :close @14:43:08.02 SSV.dial CBV @14:43:08.05
CBV.dial SSV @14:43:08.86 Allowing 130s for 25.0ml of remaining 575.0ml @14:43:08.87 Intake.select :open @14:43:11.04 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:43:11.06 SS.reconfigure SSinhale.with maxSpeed: 35 @14:43:11.11 SS.seek 25ml @14:44:00.36 Vacuum too high -- waiting up to 40s @14:44:01.43 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:44:01.45 SS.reconfigure SSinhale.with maxSpeed: 32 @14:44:01.50 SS.seek 25ml @14:44:03.29 Stroke completed -- waiting for pressure to equalize ... @14:44:06.51 Intake.select :close @14:44:06.66 SSV.dial :exhaust @14:44:07.46 Exhaust.select :open @14:44:07.57 SS.reconfigure SSexhale @14:44:07.63 SS.seek 0ml @14:44:16.90 Exhaust.select :close @14:44:17.02 SSV.dial CBV @14:44:17.05
CBV.dial SSV @14:44:17.85 Allowing 130s for 25.0ml of remaining 550.0ml @14:44:17.86 Intake.select :open @14:44:20.55 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:44:20.57 SS.reconfigure SSinhale.with maxSpeed: 32 @14:44:20.62 SS.seek 25ml @14:45:15.40 Stroke completed -- waiting for pressure to equalize ... @14:45:19.26 Intake.select :close @14:45:19.42 SSV.dial :exhaust @14:45:20.21 Exhaust.select :open @14:45:20.32 SS.reconfigure SSexhale @14:45:20.37 SS.seek 0ml @14:45:29.34 Exhaust.select :close @14:45:29.46 SSV.dial CBV @14:45:29.49
CBV.dial SSV @14:45:30.30 Allowing 130s for 25.0ml of remaining 525.0ml @14:45:30.31 Intake.select :open @14:45:32.48 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:45:32.50 SS.reconfigure SSinhale.with maxSpeed: 32 @14:45:32.55 SS.seek 25ml @14:46:27.88 Stroke completed -- waiting for pressure to equalize ... @14:46:31.75 Intake.select :close @14:46:31.91 SSV.dial :exhaust @14:46:32.69 Exhaust.select :open @14:46:32.80 SS.reconfigure SSexhale @14:46:32.85 SS.seek 0ml @14:46:41.73 Exhaust.select :close @14:46:41.85 SSV.dial CBV @14:46:41.88
CBV.dial SSV @14:46:42.69 Allowing 130s for 25.0ml of remaining 500.0ml @14:46:42.70 Intake.select :open @14:46:44.87 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:46:44.89 SS.reconfigure SSinhale.with maxSpeed: 32 @14:46:44.94 SS.seek 25ml @14:47:40.27 Stroke completed -- waiting for pressure to equalize ... @14:47:44.09 Intake.select :close @14:47:44.24 SSV.dial :exhaust @14:47:45.03 Exhaust.select :open @14:47:45.15 SS.reconfigure SSexhale @14:47:45.20 SS.seek 0ml @14:47:54.52 Exhaust.select :close @14:47:54.64 SSV.dial CBV @14:47:54.67
CBV.dial SSV @14:47:55.47 Allowing 130s for 25.0ml of remaining 475.0ml @14:47:55.48 Intake.select :open @14:47:57.65 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:47:57.67 SS.reconfigure SSinhale.with maxSpeed: 32 @14:47:57.72 SS.seek 25ml @14:48:52.42 Stroke completed -- waiting for pressure to equalize ... @14:48:56.25 Intake.select :close @14:48:56.40 SSV.dial :exhaust @14:48:57.19 Exhaust.select :open @14:48:57.30 SS.reconfigure SSexhale @14:48:57.36 SS.seek 0ml @14:49:06.34 Exhaust.select :close @14:49:06.46 SSV.dial CBV @14:49:07.01
CBV.dial SSV @14:49:07.83 Allowing 130s for 25.0ml of remaining 450.0ml @14:49:07.84 Intake.select :open @14:49:10.02 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:49:10.03 SS.reconfigure SSinhale.with maxSpeed: 32 @14:49:10.08 SS.seek 25ml @14:50:05.10 Stroke completed -- waiting for pressure to equalize ... @14:50:08.97 Intake.select :close @14:50:09.13 SSV.dial :exhaust @14:50:09.93 Exhaust.select :open @14:50:10.04 SS.reconfigure SSexhale @14:50:10.09 SS.seek 0ml @14:50:19.26 Exhaust.select :close @14:50:19.38 SSV.dial CBV @14:50:19.41
CBV.dial SSV @14:50:20.22 Allowing 130s for 25.0ml of remaining 425.0ml @14:50:20.23 Intake.select :open @14:50:22.40 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:50:22.42 SS.reconfigure SSinhale.with maxSpeed: 32 @14:50:22.47 SS.seek 25ml @14:51:17.31 Stroke completed -- waiting for pressure to equalize ... @14:51:21.15 Intake.select :close @14:51:21.31 SSV.dial :exhaust @14:51:22.10 Exhaust.select :open @14:51:22.21 SS.reconfigure SSexhale @14:51:22.26 SS.seek 0ml @14:51:31.31 Exhaust.select :close @14:51:31.43 SSV.dial CBV @14:51:31.46
CBV.dial SSV @14:51:32.28 Allowing 130s for 25.0ml of remaining 400.0ml @14:51:32.29 Intake.select :open @14:51:34.47 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:51:34.48 SS.reconfigure SSinhale.with maxSpeed: 32 @14:51:34.53 SS.seek 25ml @14:52:29.52 Stroke completed -- waiting for pressure to equalize ... @14:52:33.35 Intake.select :close @14:52:33.50 SSV.dial :exhaust @14:52:34.29 Exhaust.select :open @14:52:34.41 SS.reconfigure SSexhale @14:52:34.46 SS.seek 0ml @14:52:43.59 Exhaust.select :close @14:52:43.71 SSV.dial CBV @14:52:43.74
CBV.dial SSV @14:52:44.57 Allowing 130s for 25.0ml of remaining 375.0ml @14:52:44.58 Intake.select :open @14:52:46.75 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:52:46.77 SS.reconfigure SSinhale.with maxSpeed: 32 @14:52:46.82 SS.seek 25ml @14:53:41.82 Stroke completed -- waiting for pressure to equalize ... @14:53:46.28 Intake.select :close @14:53:46.43 SSV.dial :exhaust @14:53:47.74 Exhaust.select :open @14:53:47.86 SS.reconfigure SSexhale @14:53:47.91 SS.seek 0ml @14:53:57.01 Exhaust.select :close @14:53:57.13 SSV.dial CBV @14:53:57.16
CBV.dial SSV @14:53:57.97 Allowing 130s for 25.0ml of remaining 350.0ml @14:53:57.98 Intake.select :open @14:54:00.16 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:54:00.17 SS.reconfigure SSinhale.with maxSpeed: 32 @14:54:00.22 SS.seek 25ml @14:54:46.12 Vacuum too high -- waiting up to 40s @14:54:47.22 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:54:47.24 SS.reconfigure SSinhale.with maxSpeed: 29 @14:54:47.29 SS.seek 25ml @14:54:57.43 Stroke completed -- waiting for pressure to equalize ... @14:55:01.31 Intake.select :close @14:55:01.47 SSV.dial :exhaust @14:55:02.25 Exhaust.select :open @14:55:02.36 SS.reconfigure SSexhale @14:55:02.41 SS.seek 0ml @14:55:11.45 Exhaust.select :close @14:55:11.57 SSV.dial CBV @14:55:11.60
CBV.dial SSV @14:55:12.43 Allowing 130s for 25.0ml of remaining 325.0ml @14:55:12.44 Intake.select :open @14:55:14.61 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:55:14.63 SS.reconfigure SSinhale.with maxSpeed: 29 @14:55:14.68 SS.seek 25ml @14:56:15.21 Stroke completed -- waiting for pressure to equalize ... @14:56:19.06 Intake.select :close @14:56:19.22 SSV.dial :exhaust @14:56:20.00 Exhaust.select :open @14:56:20.11 SS.reconfigure SSexhale @14:56:20.16 SS.seek 0ml @14:56:28.95 Exhaust.select :close @14:56:29.07 SSV.dial CBV @14:56:29.10
CBV.dial SSV @14:56:29.93 Allowing 130s for 25.0ml of remaining 300.0ml @14:56:29.94 Intake.select :open @14:56:32.11 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:56:32.13 SS.reconfigure SSinhale.with maxSpeed: 29 @14:56:32.18 SS.seek 25ml @14:57:33.04 Stroke completed -- waiting for pressure to equalize ... @14:57:36.89 Intake.select :close @14:57:37.05 SSV.dial :exhaust @14:57:37.82 Exhaust.select :open @14:57:37.94 SS.reconfigure SSexhale @14:57:37.99 SS.seek 0ml @14:57:47.04 Exhaust.select :close @14:57:47.16 SSV.dial CBV @14:57:47.19
CBV.dial SSV @14:57:47.99 Allowing 130s for 25.0ml of remaining 275.0ml @14:57:48.00 Intake.select :open @14:57:50.17 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:57:50.19 SS.reconfigure SSinhale.with maxSpeed: 29 @14:57:50.76 SS.seek 25ml @14:58:51.34 Stroke completed -- waiting for pressure to equalize ... @14:58:55.85 Intake.select :close @14:58:56.01 SSV.dial :exhaust @14:58:56.81 Exhaust.select :open @14:58:56.92 SS.reconfigure SSexhale @14:58:56.97 SS.seek 0ml @14:59:05.81 Exhaust.select :close @14:59:05.93 SSV.dial CBV @14:59:05.98
CBV.dial SSV @14:59:06.77 Allowing 130s for 25.0ml of remaining 250.0ml @14:59:06.78 Intake.select :open @14:59:08.97 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:59:08.98 SS.reconfigure SSinhale.with maxSpeed: 29 @14:59:09.03 SS.seek 25ml @15:00:09.93 Stroke completed -- waiting for pressure to equalize ... @15:00:14.41 Intake.select :close @15:00:14.57 SSV.dial :exhaust @15:00:15.36 Exhaust.select :open @15:00:15.47 SS.reconfigure SSexhale @15:00:15.52 SS.seek 0ml @15:00:24.51 Exhaust.select :close @15:00:24.63 SSV.dial CBV @15:00:24.66
CBV.dial SSV @15:00:25.49 Allowing 130s for 25.0ml of remaining 225.0ml @15:00:25.50 Intake.select :open @15:00:27.68 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:00:27.69 SS.reconfigure SSinhale.with maxSpeed: 29 @15:00:27.74 SS.seek 25ml @15:01:28.26 Stroke completed -- waiting for pressure to equalize ... @15:01:32.69 Intake.select :close @15:01:32.85 SSV.dial :exhaust @15:01:33.65 Exhaust.select :open @15:01:33.76 SS.reconfigure SSexhale @15:01:33.81 SS.seek 0ml @15:01:42.90 Exhaust.select :close @15:01:43.02 SSV.dial CBV @15:01:43.05
CBV.dial SSV @15:01:43.85 Allowing 130s for 25.0ml of remaining 200.0ml @15:01:43.86 Intake.select :open @15:01:46.03 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:01:46.05 SS.reconfigure SSinhale.with maxSpeed: 29 @15:01:46.10 SS.seek 25ml @15:02:46.70 Stroke completed -- waiting for pressure to equalize ... @15:02:51.15 Intake.select :close @15:02:51.30 SSV.dial :exhaust @15:02:52.63 Exhaust.select :open @15:02:52.75 SS.reconfigure SSexhale @15:02:52.80 SS.seek 0ml @15:03:01.65 Exhaust.select :close @15:03:01.77 SSV.dial CBV @15:03:01.80
CBV.dial SSV @15:03:02.63 Allowing 130s for 25.0ml of remaining 175.0ml @15:03:02.64 Intake.select :open @15:03:04.82 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:03:04.83 SS.reconfigure SSinhale.with maxSpeed: 29 @15:03:04.88 SS.seek 25ml @15:04:05.93 Stroke completed -- waiting for pressure to equalize ... @15:04:10.43 Intake.select :close @15:04:10.59 SSV.dial :exhaust @15:04:11.38 Exhaust.select :open @15:04:11.49 SS.reconfigure SSexhale @15:04:11.54 SS.seek 0ml @15:04:20.81 Exhaust.select :close @15:04:20.93 SSV.dial CBV @15:04:20.96
CBV.dial SSV @15:04:21.77 Allowing 130s for 25.0ml of remaining 150.0ml @15:04:21.78 Intake.select :open @15:04:23.95 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:04:23.97 SS.reconfigure SSinhale.with maxSpeed: 29 @15:04:24.02 SS.seek 25ml @15:04:58.95 Vacuum too high -- waiting up to 40s @15:05:00.04 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:05:00.06 SS.reconfigure SSinhale.with maxSpeed: 26 @15:05:00.11 SS.seek 25ml @15:05:28.95 Stroke completed -- waiting for pressure to equalize ... @15:05:33.44 Intake.select :close @15:05:33.60 SSV.dial :exhaust @15:05:34.40 Exhaust.select :open @15:05:34.54 SS.reconfigure SSexhale @15:05:34.59 SS.seek 0ml @15:05:43.49 Exhaust.select :close @15:05:43.61 SSV.dial CBV @15:05:43.64
CBV.dial SSV @15:05:44.46 Allowing 130s for 25.0ml of remaining 125.0ml @15:05:44.47 Intake.select :open @15:05:46.64 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:05:46.66 SS.reconfigure SSinhale.with maxSpeed: 26 @15:05:46.71 SS.seek 25ml @15:06:54.48 Stroke completed -- waiting for pressure to equalize ... @15:06:58.92 Intake.select :close @15:06:59.07 SSV.dial :exhaust @15:06:59.87 Exhaust.select :open @15:06:59.98 SS.reconfigure SSexhale @15:07:00.03 SS.seek 0ml @15:07:09.15 Exhaust.select :close @15:07:09.27 SSV.dial CBV @15:07:09.80
CBV.dial SSV @15:07:10.64 Allowing 130s for 25.0ml of remaining 100.0ml @15:07:10.65 Intake.select :open @15:07:12.83 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:07:12.84 SS.reconfigure SSinhale.with maxSpeed: 26 @15:07:12.89 SS.seek 25ml @15:08:20.29 Stroke completed -- waiting for pressure to equalize ... @15:08:25.43 Intake.select :close @15:08:25.59 SSV.dial :exhaust @15:08:26.38 Exhaust.select :open @15:08:26.49 SS.reconfigure SSexhale @15:08:26.54 SS.seek 0ml @15:08:35.92 Exhaust.select :close @15:08:36.04 SSV.dial CBV @15:08:36.07
CBV.dial SSV @15:08:36.88 Allowing 130s for 25.0ml of remaining 75.0ml @15:08:36.89 Intake.select :open @15:08:39.07 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:08:39.08 SS.reconfigure SSinhale.with maxSpeed: 26 @15:08:39.13 SS.seek 25ml @15:09:46.59 Stroke completed -- waiting for pressure to equalize ... @15:09:51.09 Intake.select :close @15:09:51.25 SSV.dial :exhaust @15:09:52.03 Exhaust.select :open @15:09:52.14 SS.reconfigure SSexhale @15:09:52.19 SS.seek 0ml @15:10:01.29 Exhaust.select :close @15:10:01.41 SSV.dial CBV @15:10:01.44
CBV.dial SSV @15:10:02.25 Allowing 130s for 25.0ml of remaining 50.0ml @15:10:02.26 Intake.select :open @15:10:04.44 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:10:04.45 SS.reconfigure SSinhale.with maxSpeed: 26 @15:10:04.50 SS.seek 25ml @15:11:11.95 Stroke completed -- waiting for pressure to equalize ... @15:11:17.04 Intake.select :close @15:11:17.19 SSV.dial :exhaust @15:11:17.99 Exhaust.select :open @15:11:18.10 SS.reconfigure SSexhale @15:11:18.15 SS.seek 0ml @15:11:26.96 Exhaust.select :close @15:11:27.08 SSV.dial CBV @15:11:27.11
CBV.dial SSV @15:11:27.93 Allowing 130s for 25.0ml of remaining 25.0ml @15:11:27.94 Intake.select :open @15:11:30.11 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:11:30.13 SS.reconfigure SSinhale.with maxSpeed: 26 @15:11:30.18 SS.seek 24.96ml @15:12:37.70 Stroke completed -- waiting for pressure to equalize ... @15:12:42.72 Intake.select :close @15:12:42.88 SSV.dial :exhaust @15:12:43.67 Exhaust.select :open @15:12:43.78 SS.reconfigure SSexhale @15:12:43.83 SS.seek 0ml @15:12:52.99 Exhaust.select :close @15:12:53.11 Sampled 1500.0ml @15:12:53.13 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @15:12:53.14 Intake.select :close @15:12:53.21 CSV.dial :waste @15:12:53.29 CS.seek 0ml @15:12:53.37 SSV.dial CBV,avoiding: :exhaust @15:12:53.40
CBV.dial SSV @15:12:53.48 CSV.dial :air @15:12:54.25 CS.seek 10ml @15:12:55.35
CTV.dial :air @15:12:56.11 delay 15 seconds @15:13:11.14 SSV.dial :spit,avoiding: :exhaust @15:13:12.49 CTV.dial CSV,avoiding: :intake @15:13:12.58
SS.seek 0ml @15:13:12.66 SSV.dial CBV,avoiding: :exhaust @15:13:18.32 CBV.dial between SSV and waste2 @15:13:18.75 CTV.dial CSV,avoiding: :air @15:13:18.78
CSV.dial CTV @15:13:19.26 CS.reconfigure CSpressurize @15:13:19.32 CS.seek 0ml @15:13:44.75 BubblePoint: Intake @68.9psia, Outlet @13.3psia @15:13:44.76 CBV.dial SSV @15:13:45.12 SS.reconfigure SSconfig @15:13:45.17 SS.seek 10ml @15:13:50.77 delay 15 seconds @15:14:05.92 Evacuated: Intake @33.6psia, Outlet @-0.2psia @15:14:05.94 SSV.dial :spit,avoiding: :exhaust @15:14:07.30 SS.reconfigure SSexhale @15:14:07.35 SS.seek 0ml @15:14:12.23 SS.reconfigure SSfast @15:14:12.29 SS.seek 5ml @15:14:14.99 SSV.dial CBV,avoiding: :exhaust @15:14:15.02
CBV.dial SSV @15:14:16.38 CTV.dial :air,avoiding: :intake @15:14:17.73 CSV.dial CTV @15:14:17.78
SS.seek 10ml @15:14:18.77 CSV.dial :air @15:14:19.27 CS.reconfigure CSfast @15:14:19.32 CS.seek 0ml @15:14:20.24
SSV.dial :spit,avoiding: :exhaust @15:14:21.00 CS.coast @15:14:21.58
SS.reconfigure SSexhale @15:14:21.63 SS.seek 0ml @15:14:26.67 SS.reconfigure SSfast @15:14:26.72 SS.seek 5ml @15:14:29.42 SSV.dial CBV,avoiding: :exhaust @15:14:29.45
CBV.dial SSV @15:14:31.83 SSV.dial :spit,avoiding: :exhaust @15:14:33.19 SS.reconfigure SSconfig @15:14:33.24 SS.seek 0ml @15:14:36.38 SS.coast @15:14:36.43 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @15:14:36.44 Intake.select :close @15:14:36.52 CSV.dial :waste @15:14:37.19 SSV.dial CBV,avoiding: :exhaust @15:14:37.21
CBV.dial SSV @15:14:37.35 CS.seek 0ml @15:14:37.43 CSV.dial :air @15:14:38.30 CS.seek 10ml @15:14:38.57
CTV.dial :air @15:14:38.58 delay 15 seconds @15:14:53.61 SSV.dial :spit,avoiding: :exhaust @15:14:54.97 CTV.dial CSV,avoiding: :intake @15:14:55.06
SS.reconfigure SSexhale @15:14:55.11 SS.seek 0ml @15:14:55.39 SSV.dial CBV,avoiding: :exhaust @15:15:01.76 CBV.dial between SSV and waste2 @15:15:02.19 CSV.dial between air and CTV @15:15:02.55 CS.seek 8.248ml @15:15:07.51 CTV.dial CSV,avoiding: :air @15:15:07.54
CSV.dial CTV @15:15:07.88 CS.reconfigure CSpressurize @15:15:07.93 CS.seek 0ml @15:15:29.66 CSV.dial :air @15:15:30.12 CS.reconfigure CSfast @15:15:30.17 CS.seek 10ml @15:15:53.57 CSV.dial between air and CTV @15:15:53.91 CS.seek 3.123ml @15:16:10.51 CTV.dial CSV,avoiding: :air @15:16:10.54
CSV.dial CTV @15:16:10.87 CS.reconfigure CSpressurize @15:16:10.92 CS.seek 0ml @15:16:18.75 BubblePoint: Intake @68.0psia, Outlet @12.7psia @15:16:18.76 CBV.dial SSV @15:16:19.12 SS.reconfigure SSconfig @15:16:19.17 SS.seek 10ml @15:16:24.66 delay 15 seconds @15:16:39.80 Evacuated: Intake @37.3psia, Outlet @14.8psia @15:16:39.82 SSV.dial :spit,avoiding: :exhaust @15:16:41.17 SS.reconfigure SSexhale @15:16:41.22 SS.seek 0ml @15:16:46.37 SS.reconfigure SSfast @15:16:46.42 SS.seek 5ml @15:16:49.11 SSV.dial CBV,avoiding: :exhaust @15:16:49.14
CBV.dial SSV @15:16:50.50 CTV.dial :air,avoiding: :intake @15:16:51.84 CSV.dial CTV @15:16:51.89
SS.seek 10ml @15:16:52.88 CSV.dial :air @15:16:53.37 CS.reconfigure CSfast @15:16:53.42 CS.seek 0ml @15:16:54.81
SSV.dial :spit,avoiding: :exhaust @15:16:55.19 CS.coast @15:16:56.16
SS.reconfigure SSexhale @15:16:56.21 SS.seek 0ml @15:17:01.12 SS.reconfigure SSfast @15:17:01.17 SS.seek 5ml @15:17:03.97 SSV.dial CBV,avoiding: :exhaust @15:17:04.00
CBV.dial SSV @15:17:06.39 SSV.dial :spit,avoiding: :exhaust @15:17:07.74 SS.reconfigure SSconfig @15:17:07.79 SS.seek 0ml @15:17:11.23 SS.coast @15:17:11.28 Lysing: Adding 1.5ml lysis @15:17:11.29 CBV.dial between CSV and waste1,avoiding: :DBV @15:17:12.81 CTV.dial CSV @15:17:14.15 CSV.dial CSR @15:17:14.96 Pulling 1ml of CSR.lysis @15:17:14.97 CSR.select :lysis @15:17:15.09 CS.reconfigure CSconfig @15:17:15.15 CS.seek 1ml @15:17:18.73 delay 5 seconds @15:17:23.75 CSR.select :air @15:17:23.94 CS.seek 6ml @15:17:37.67 delay 2 seconds @15:17:39.68 CSV.dial :waste1 @15:17:41.01 CS.reconfigure CSfast @15:17:41.06 CS.seek :empty @15:17:55.48 CSV.dial CSR @15:17:56.85 Pulling 1.5ml of CSR.lysis @15:17:56.86 CSR.select :lysis @15:17:56.99 CS.reconfigure CSconfig @15:17:57.03 CS.seek 1.5ml @15:18:01.92 delay 5 seconds @15:18:06.95 CSR.select :air @15:18:07.13 CS.seek 4.501ml @15:18:15.85 delay 2 seconds @15:18:17.86 CSV.dial CTV @15:18:18.36 CS.seek 1.275ml @15:18:27.60 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @15:18:27.62 CH.seek 85C,8:00 @15:21:13.86 CH.hold 5:00,85C @15:26:14.03 Collection Puck Heater at 85C after lysing @15:26:14.05 CS.seek 0ml @15:26:18.27 CTV.dial between spare and air,avoiding: :intake @15:26:19.34 CSV.dial :air @15:26:19.81 CS.seek 5ml @15:26:33.54 CSV.dial CTV @15:26:34.03 delay 2 seconds @15:26:36.05 CSV.dial :air @15:26:36.53 delay 2 seconds @15:26:38.55 CSV.dial CTV @15:26:39.03 delay 2 seconds @15:26:41.06 CSV.dial :air @15:26:41.53 delay 2 seconds @15:26:43.56 CS.seek 0ml @15:26:57.39 Recovering Lysate into Collection syringe @15:26:57.40 CSV.dial CBV @15:26:58.44 CBV.dial CSV @15:26:58.87 CS.seek 4.999ml @15:27:12.55 delay 5 seconds @15:27:17.57 CTV.dial :air @15:27:18.13 CS.seek 6.999ml @15:27:24.30 delay 5 seconds @15:27:29.32 CSV.dial :air2 @15:27:29.78 CS.seek 10ml @15:27:38.52 CSV.dial between air and CTV,avoiding: CBV @15:27:39.69 Lysing Complete @15:27:39.77 Storing sh1 puck in Collection Clamp to tube 1 @15:27:39.97 SC.reconfigure SCtight @15:27:40.02 SC.seek 1 @15:27:42.25 SC.reconfigure SCloose @15:27:42.48 Elbow.reconfigure TightElbow @15:27:42.53 Elbow.seek 1 @15:27:44.41 Forearm.reconfigure TightForearm @15:27:44.46 Forearm.seek 1 + 700 counts @15:27:45.48 SE.reconfigure SEpick @15:27:45.55 SE.seek beamBreak @15:27:47.05 SE.configure SEconfig @15:27:47.21 Elbow.reconfigure LimpElbow @15:27:47.26 Forearm.reconfigure LooseForearm @15:27:47.31 Forearm.seek 1 @15:27:47.63 Elbow.hold @15:27:47.70 Elbow.reconfigure TightElbow @15:27:47.81 SE.seek 17.09 Pucks below Top Plate @15:27:48.35 Forearm.force -20 @15:27:48.39 Forearm.seek 1 + 349 counts @15:27:48.71 Forearm.reconfigure TightForearm @15:27:48.76 Forearm.seek 1 + 700 counts @15:27:49.02 SE.seek 18.09 Pucks below Top Plate @15:27:49.70 Forearm.reconfigure LooseForearm @15:27:49.75 Forearm.seek :retract @15:27:50.64 Elbow.seek CC @15:27:52.34 Elbow.reconfigure LooseElbow @15:27:52.44 Forearm.seek CC @15:27:53.41 Hand.close @15:27:54.08 CC.seek :open @15:27:58.61 Forearm.seek :retract @15:27:59.60 Elbow.reconfigure TightElbow @15:27:59.65 Elbow.seek 1 @15:28:02.71 Elbow.reconfigure LimpElbow @15:28:02.77 Forearm.seek 1 @15:28:03.83 Elbow.hold @15:28:03.90 Elbow.reconfigure TightElbow @15:28:03.95 Hand.open @15:28:04.71 Forearm.force -20 @15:28:04.75 Forearm.seek 1 + 343 counts @15:28:05.08 Forearm.reconfigure TightForearm @15:28:05.14 Forearm.seek 1 + 700 counts @15:28:05.46 SE.seek :down @15:28:06.55 SE.coast @15:28:06.67
Forearm.reconfigure LooseForearm @15:28:06.72 Forearm.seek :retract @15:28:07.63 SC.coast @15:28:07.67 Tranferring lysate to PRV @15:28:07.68 CSV.dial :air @15:28:08.01 CS.seek 10ml @15:28:08.08 CSV.dial between air and CTV @15:28:08.43 AIV.dial :bypass @15:28:08.52 Beginning samplePassC @15:28:08.53 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:28:08.61 Hand.open @15:28:08.98 Elbow.seek FlushPuck::Garage @15:28:10.84 Elbow.reconfigure LooseElbow @15:28:10.95 Forearm.seek :garage @15:28:11.96 Hand.close @15:28:12.71 Forearm.seek :retract @15:28:13.72 Elbow.reconfigure TightElbow @15:28:13.77 Elbow.seek CC @15:28:16.67 Elbow.reconfigure LooseElbow @15:28:16.78 Forearm.reconfigure TightForearm @15:28:16.82 Forearm.seek CC @15:28:18.11 CC.seek :closed @15:28:22.36 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.93 @15:28:22.43 Hand.open @15:28:23.15 Forearm.reconfigure LooseForearm @15:28:23.20 Forearm.seek :retract @15:28:24.18 AWV.select :air @15:28:24.20 AIV.dial :bypass @15:28:24.21 PSV.dial PSR @15:28:25.61 Pulling 1.5ml of PSR.flush @15:28:25.62 PSR.select :flush @15:28:25.75 PS.seek 1.5ml @15:28:41.77 delay 5 seconds @15:28:46.79 PSV.dial :waste1 @15:28:47.82 PS.seek 0ml @15:29:03.85 PSV.dial PSR @15:29:04.33 PSR.select :air @15:29:04.46 PS.seek 2.5ml @15:29:30.64 PRV.dial CBV @15:29:30.67
CBV.dial PRV @15:29:31.52 PSV.dial :mixloop @15:29:32.55 CTV.dial :air,avoiding: :intake @15:29:32.56 PTV.dial :PRVmixing @15:29:32.64 PS.seek 0ml @15:29:58.76 PSV.dial :air @15:29:59.22 PS.seek 2.5ml @15:30:25.42 PSV.dial :mixloop @15:30:25.89 PS.seek 0ml @15:30:52.07 PSV.dial :air @15:30:52.53 PS.seek 2.5ml @15:31:18.70 PSV.dial :mixloop @15:31:19.18 PS.seek 0ml @15:31:45.33 Garaging FlushPuck in Collection Clamp @15:31:45.41 Elbow.reconfigure TightElbow @15:31:45.46 Elbow.seek CC @15:31:45.69 Elbow.reconfigure LooseElbow @15:31:45.80 Forearm.seek CC @15:31:46.81 Hand.close @15:31:47.46 CC.seek :open @15:31:51.84 Forearm.seek :retract @15:31:52.84 Elbow.reconfigure TightElbow @15:31:52.89 Elbow.seek :garage @15:31:56.08 Elbow.reconfigure LooseElbow @15:31:56.19 Forearm.reconfigure TightForearm @15:31:56.24 Forearm.seek :garage @15:31:57.39 Hand.open @15:31:58.03 Forearm.reconfigure LooseForearm @15:31:58.08 Forearm.seek :retract @15:31:59.12 Connecting PS to CS to transfer sample @15:31:59.13 CBV.dial AIV @15:31:59.14 AIV.dial :bypass @15:31:59.15 PSV.dial :mixloop @15:31:59.17 PTV.dial :PRVmixing @15:31:59.18 PRV.dial AIV @15:31:59.19 CTV.dial :air,avoiding: :intake @15:31:59.21 Loading lyfil puck from tube 2 into Collection Clamp @15:31:59.47 SC.reconfigure SCtight @15:31:59.52 SC.seek 2 @15:32:01.58 SC.reconfigure SCloose @15:32:01.80 Elbow.reconfigure TightElbow @15:32:01.85 Elbow.seek 2 @15:32:03.90 Forearm.reconfigure TightForearm @15:32:03.95 Forearm.seek 2 + 700 counts @15:32:04.95 SE.reconfigure SEpick @15:32:05.02 SE.seek beamBreak @15:32:07.95 SE.configure SEconfig @15:32:08.13 Elbow.seek 2 @15:32:08.32 Forearm.seek 2 + 300 counts @15:32:08.59 Forearm.reconfigure LooseForearm @15:32:08.63 Hand.close @15:32:09.86 SE.seek :down @15:32:10.09
Forearm.seek :retract @15:32:11.02 Elbow.seek CC @15:32:12.59 SE.coast @15:32:12.73
Elbow.reconfigure LooseElbow @15:32:12.83 Forearm.reconfigure TightForearm @15:32:12.88 Forearm.seek CC @15:32:14.13 CC.seek :closed @15:32:18.40 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.95 @15:32:18.41 Hand.open @15:32:19.16 Forearm.reconfigure LooseForearm @15:32:19.20 Forearm.seek :retract @15:32:20.20 SC.coast @15:32:20.24 CTV.dial CSV,avoiding: :intake @15:32:21.60 CSV.dial CTV @15:32:21.92 CS.seek 5ml @15:32:35.71 delay 1:20 @15:33:55.74 CS.seek 2.5ml @15:34:03.15 delay 40 seconds @15:34:43.18 CS.seek 0ml @15:34:50.59 delay 1:00 @15:35:50.61 PSV.dial :air @15:35:51.10 PS.seek 2.5ml @15:36:17.29 PSV.dial :mixloop @15:36:17.76 delay 3 seconds @15:36:20.78 PSV.dial :air @15:36:21.26 delay 2 seconds @15:36:23.27 PSV.dial :mixloop @15:36:23.74 delay 3 seconds @15:36:26.76 PSV.dial :waste1 @15:36:28.09 PS.seek :empty @15:36:54.17 CBV.dial :waste1 @15:36:54.65 delay 5 seconds @15:36:59.67 CBV.dial AIV @15:37:00.18 delay 10 seconds @15:37:10.20 CBV.dial :waste1 @15:37:10.70 delay 10 seconds @15:37:20.72 CBV.dial AIV @15:37:21.21 delay 10 seconds @15:37:31.24 CTV.dial CSV,avoiding: :intake @15:37:31.25 CBV.dial between AIV and waste1 @15:37:31.60 CSV.dial :air @15:37:32.07 CS.seek 10ml @15:37:58.58 CSV.dial CTV @15:37:59.07 CS.seek 0ml @15:38:25.52 CBV.dial :waste1 @15:38:25.84 delay 10 seconds @15:38:35.87 CTV.dial :air,avoiding: :intake @15:38:37.26 Storing lyfil puck in Collection Clamp to tube 1 @15:38:37.45 SC.reconfigure SCtight @15:38:37.50 SC.seek 1 @15:38:39.67 SC.reconfigure SCloose @15:38:39.89 Elbow.reconfigure TightElbow @15:38:39.94 Elbow.seek 1 @15:38:41.91 Forearm.reconfigure TightForearm @15:38:41.96 Forearm.seek 1 + 700 counts @15:38:43.48 SE.reconfigure SEpick @15:38:43.64 SE.seek beamBreak @15:38:44.95 SE.configure SEconfig @15:38:45.12 Elbow.reconfigure LimpElbow @15:38:45.18 Forearm.reconfigure LooseForearm @15:38:45.23 Forearm.seek 1 @15:38:45.53 Elbow.hold @15:38:45.60 Elbow.reconfigure TightElbow @15:38:45.71 SE.seek 18.1 Pucks below Top Plate @15:38:46.25 Forearm.force -20 @15:38:46.29 Forearm.seek 1 + 345 counts @15:38:46.61 Forearm.reconfigure TightForearm @15:38:46.67 Forearm.seek 1 + 700 counts @15:38:47.01 SE.seek 19.1 Pucks below Top Plate @15:38:47.68 Forearm.reconfigure LooseForearm @15:38:47.73 Forearm.seek :retract @15:38:48.66 Elbow.seek CC @15:38:50.41 Elbow.reconfigure LooseElbow @15:38:50.52 Forearm.seek CC @15:38:51.48 Hand.close @15:38:52.11 CC.seek :open @15:38:56.59 Forearm.seek :retract @15:38:57.59 Elbow.reconfigure TightElbow @15:38:57.64 Elbow.seek 1 @15:38:59.51 Elbow.reconfigure LimpElbow @15:38:59.56 Forearm.seek 1 @15:39:00.58 Elbow.hold @15:39:00.65 Elbow.reconfigure TightElbow @15:39:00.70 Hand.open @15:39:01.48 Forearm.force -20 @15:39:01.53 Forearm.seek 1 + 350 counts @15:39:01.84 Forearm.reconfigure TightForearm @15:39:01.89 Forearm.seek 1 + 700 counts @15:39:02.22 SE.seek :down @15:39:03.17 SE.coast @15:39:03.37
Forearm.reconfigure LooseForearm @15:39:03.41 Forearm.seek :retract @15:39:04.31 SC.coast @15:39:04.35 PSV.dial :air @15:39:05.45 PS.seek 0.499ml @15:39:11.34 delay 3 seconds @15:39:14.36 PSV.dial :mixloop @15:39:14.82 PRV.dial :air @15:39:15.29 delay 3 seconds @15:39:18.30 PRV.dial CBV @15:39:18.79 CBV.dial AIV @15:39:19.28 PRV.dial between CBV and air @15:39:19.62 PRV.dial :air @15:39:20.02 PS.reconfigure PSslow @15:39:20.07 PS.seek 0.599ml @15:39:22.55 PRV.dial :waste2 @15:39:23.31 PS.reconfigure PSconfig @15:39:23.37 PS.seek :empty @15:39:30.84 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:39:30.92 Hand.open @15:39:31.35 Elbow.seek FlushPuck::Garage @15:39:33.37 Elbow.reconfigure LooseElbow @15:39:33.52 Forearm.seek :garage @15:39:34.53 Hand.close @15:39:35.23 Forearm.seek :retract @15:39:36.24 Elbow.reconfigure TightElbow @15:39:36.29 Elbow.seek CC @15:39:39.11 Elbow.reconfigure LooseElbow @15:39:39.22 Forearm.reconfigure TightForearm @15:39:39.27 Forearm.seek CC @15:39:40.57 CC.seek :closed @15:39:44.82 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.93 @15:39:44.92 Hand.open @15:39:45.67 Forearm.reconfigure LooseForearm @15:39:45.71 Forearm.seek :retract @15:39:46.76 Rinsing MixLoop with GuSCN @15:39:46.77 PSV.dial :mixloop,avoiding: PBV @15:39:46.78 PTV.dial :PRVmixing,avoiding: :puck @15:39:46.80 PRV.dial :spare1 @15:39:47.91 PS.seek 1.099ml @15:39:59.87 delay 2 seconds @15:40:01.88 PRV.dial :air @15:40:02.94 PS.seek 2.5ml @15:40:18.02 PSV.dial :waste1,avoiding: PBV @15:40:19.35 PS.seek :empty @15:40:45.43 Diluting Lysate in Syringe @15:40:45.44 PSV.dial :PTV,avoiding: PBV @15:40:47.04 PTV.dial :PRV,avoiding: :puck @15:40:47.79 CBV.dial :PRV @15:40:47.80 PRV.dial :air @15:40:47.81 delay 2 seconds @15:40:49.83 PRV.dial CBV @15:40:50.31 delay 2 seconds @15:40:52.33 PS.reconfigure PSslow1 @15:40:52.39 PS.seek 0.1ml @15:41:03.34 delay 5 seconds @15:41:08.36 PRV.dial :spare1 @15:41:09.12 PS.reconfigure PSconfig @15:41:09.17 PS.seek 1ml @15:41:19.15 delay 2 seconds @15:41:21.17 PRV.dial :air @15:41:22.21 PS.seek 2.5ml @15:41:38.15 delay 2 seconds @15:41:40.17 Parking diluted lysate in Mixing Coil @15:41:40.18 PSV.dial :mixloop @15:41:40.76 PTV.dial :PRVmixing,avoiding: :puck @15:41:41.53 PRV.dial :CBV @15:41:42.07 PS.seek 1.5ml @15:41:53.00 delay 2 seconds @15:41:55.02 PSV.dial :waste1,avoiding: PBV @15:41:56.35 PS.seek :empty @15:42:12.31 Beginning samplePassF @15:42:12.38 AWV.select :air @15:42:12.40 AIV.dial :bypass @15:42:12.41 PSV.dial PSR,avoiding: PBV @15:42:12.95 Pulling 1.5ml of PSR.flush @15:42:13.47 PSR.select :flush @15:42:13.62 PS.seek 1.5ml @15:42:29.62 delay 5 seconds @15:42:34.65 PSV.dial :waste1 @15:42:35.12 PS.seek 0ml @15:42:51.20 PSV.dial PSR @15:42:51.67 PSR.select :air @15:42:51.80 PS.seek 2.5ml @15:43:18.00 PRV.dial CBV @15:43:18.03
CBV.dial PRV @15:43:18.04 PSV.dial :PTV,via: :air @15:43:19.93 CTV.dial :air,avoiding: :intake @15:43:19.94 PTV.dial :PRV,avoiding: :puck @15:43:20.70 PS.seek 0ml @15:43:46.83 PSV.dial :air @15:43:47.57 PS.seek 2.5ml @15:44:13.67 PSV.dial :PTV @15:44:14.42 PS.seek 0ml @15:44:40.69 moving diluted lysate into CBV-AIV line @15:44:40.70 PSV.dial :air @15:44:41.45 PS.seek 2.5ml @15:45:07.62 delay 2 seconds @15:45:09.63 PSV.dial :mixloop @15:45:10.11 PTV.dial :PRVmixing,avoiding: :puck @15:45:10.87 PRV.dial CBV @15:45:10.95 PS.seek 1.8ml @15:45:18.90 delay 3 seconds @15:45:21.91 PRV.dial :waste2 @15:45:22.99 PS.seek :empty @15:45:42.12 Clearing PML for modified lysate @15:45:42.13 CTV.dial :air,avoiding: :intake @15:45:42.14 PRV.dial :waste2 @15:45:42.16 PTV.dial :PRV,avoiding: :puck @15:45:42.90 delay 2 seconds @15:45:44.93 PTV.dial :PRVmixing,avoiding: :puck @15:45:45.69 delay 2 seconds @15:45:47.70 PSV.dial :PML @15:45:47.73 delay 2 seconds @15:45:49.75 PRV.dial :air @15:45:50.51 delay 2 seconds @15:45:52.60 PS.seek 1.499ml @15:46:08.62 delay 5 seconds @15:46:13.65 PS.seek 2.5ml @15:46:24.58 PSV.dial :air @15:46:25.04 PS.seek 0ml @15:46:51.22 mixing lysate with speDil @15:46:51.23 PSV.dial :PML @15:46:51.69 CBV.dial :PRV @15:46:51.70 PRV.dial :air @15:46:51.71 delay 2 seconds @15:46:53.73 PRV.dial :speDil @15:46:54.22 delay 2 seconds @15:46:56.32 PS.reconfigure PSslow1 @15:46:56.37 PS.seek 0.225ml @15:47:19.90 delay 5 seconds @15:47:24.92 PRV.dial CBV @15:47:25.70 delay 2 seconds @15:47:27.78 PS.seek 0.475ml @15:47:53.47 delay 5 seconds @15:47:58.50 PRV.dial :air @15:47:58.98 delay 2 seconds @15:48:01.00 PS.seek 0.965ml @15:48:50.87 delay 10 seconds @15:49:00.89 PRV.dial CBV @15:49:01.37 CTV.dial :air,avoiding: :intake @15:49:01.38 PSV.dial :air @15:49:01.84 PS.reconfigure PSconfig @15:49:01.89 PS.seek 2.5ml @15:49:18.22 delay 3 seconds @15:49:21.23 PSV.dial PTV @15:49:21.98 PTV.dial :PRV,avoiding: :puck @15:49:22.75 PS.seek 0ml @15:49:48.86 PSV.dial :air @15:49:49.61 PS.seek 2.5ml @15:50:15.72 delay 3 seconds @15:50:18.73 PSV.dial PTV @15:50:19.48 PTV.dial :PRV,avoiding: :puck @15:50:19.50 PS.seek 0ml @15:50:45.63 Pushing modified lysate into AIV Injection Loop @15:50:45.64 CBV.dial AIV @15:50:45.65 AIV.dial :bypass @15:50:45.66 PRV.dial CBV @15:50:45.68 PSV.dial :air @15:50:46.95 PS.seek 2.5ml @15:51:13.03 delay 3 seconds @15:51:16.05 PSV.dial :PML @15:51:16.52 PTV.dial :PRVmixing,avoiding: :puck @15:51:17.35 PS.reconfigure PSslow1 @15:51:17.40 PS.seek 1.74ml @15:52:34.75 delay 5 seconds @15:52:39.77 AIV.dial :sample @15:52:40.38 AWV.select :waste @15:52:40.55 PS.reconfigure PSslow2 @15:52:40.60 PS.seek 1.22ml @15:54:28.50 delay 15 seconds @15:54:43.52 AIV.dial :bypass @15:54:44.12 PS.reconfigure PSconfig @15:54:44.17 PS.seek 0ml @15:54:57.34 PSV.dial :air @15:54:57.82 PS.seek 2.5ml @15:55:23.90 PSV.dial :PML @15:55:24.39 PS.seek 0ml @15:55:50.56 AIV.dial :coilToMFB @15:55:50.58 AWV.select :air @15:55:50.75 Extracting Nucleic Acid from AIV @15:55:50.78
Beginning samplePassD @15:55:50.84 SPE.hold 2:46:40,55C @15:55:50.90 ASV.dial :SPEdelivery @15:55:50.99
AWV.select :air @15:55:51.00 AIV.dial :bypass @15:55:51.02 PSV.dial PSR @15:55:51.29 delay 20 seconds @15:55:52.16
Pulling 1.5ml of PSR.killMFB @15:55:52.17 PSR.select :killMFB @15:55:52.30 PS.seek 1.5ml @15:56:08.32 delay 5 seconds @15:56:11.32 primeLysateINT @15:56:11.33 AWV.select :air @15:56:11.34 AIV.dial :coilToMFB @15:56:11.35 ATV.dial AIV @15:56:12.99 AS.reconfigure ASslow1 @15:56:13.04 AS.seek 0.299ml @15:56:13.34
PSV.dial :waste1 @15:56:13.84 PS.seek 0ml @15:56:29.82 PSV.dial PSR @15:56:30.30 PSR.select :air @15:56:30.43 PS.seek 2.5ml @15:56:56.63 PRV.dial CBV @15:56:56.66
CBV.dial PRV @15:56:56.67 PSV.dial :mixloop @15:56:57.75 CTV.dial :air,avoiding: :intake @15:56:57.76 PTV.dial :PRVmixing @15:56:57.78 PS.seek 0ml @15:57:13.76 delay 10 seconds @15:57:23.78 ATV.dial :air2 @15:57:23.96
PSV.dial PSR @15:57:24.58 AS.seek 0.329ml @15:57:25.10
Pulling 1.5ml of PSR.flush @15:57:25.11 PSR.select :flush @15:57:25.24 PS.seek 1.5ml @15:57:30.89 ATV.dial :waste2 @15:57:31.43 AS.reconfigure ASnorm @15:57:31.48 AS.seek :empty @15:57:41.29
delay 5 seconds @15:57:46.31 PSV.dial :waste1 @15:57:46.81 PS.seek 0ml @15:57:48.49 Lysate primed! @15:57:48.50 AFV.select :holdingCoil @15:57:48.51 ATV.dial :air2 @15:57:49.11 AS.reconfigure ASslow1 @15:57:49.16 AS.seek 0.069ml @15:58:02.87
PSV.dial PSR @15:58:03.35 PSR.select :air @15:58:03.48 PS.seek 2.5ml @15:58:03.59 delay 3 seconds @15:58:06.60 ATV.dial :AIV @15:58:07.41 AS.seek 0.469ml @15:58:29.69 PRV.dial CBV @15:58:29.72
CBV.dial PRV @15:58:29.73 PSV.dial :mixloop @15:58:31.33 CTV.dial :air,avoiding: :intake @15:58:31.34 PTV.dial :PRVmixing @15:58:31.37 PS.seek 0ml @15:58:57.56 PSV.dial PSR @15:58:58.69 Pulling 1.5ml of PSR.flush @15:58:58.70 PSR.select :flush @15:58:58.83 PS.seek 1.5ml @15:59:14.88 delay 5 seconds @15:59:19.91 PSV.dial :waste1 @15:59:20.41 PS.seek 0ml @15:59:28.10 delay 5 seconds @15:59:33.18 Solid Phase Extraction Heater at 50.2C @15:59:33.19 ATV.dial ASV @15:59:33.59 ASV.dial :SPEdelivery @15:59:33.67 AS.seek 0.041ml @15:59:36.43
PSV.dial PSR @15:59:36.91 PSR.select :air @15:59:37.04 PS.seek 2.5ml @16:00:03.16 PRV.dial CBV @16:00:03.19
CBV.dial PRV @16:00:03.20 PSV.dial :mixloop @16:00:04.36 CTV.dial :air,avoiding: :intake @16:00:04.37 PTV.dial :PRVmixing @16:00:04.39 PS.seek 0ml @16:00:30.50 PSV.dial PSR @16:00:31.61 Pulling 1.5ml of PSR.flush @16:00:31.62 PSR.select :flush @16:00:31.75 PS.seek 1.5ml @16:00:47.82 delay 5 seconds @16:00:52.84 PSV.dial :waste1 @16:00:53.33 PS.seek 0ml @16:01:00.03 delay 10 seconds @16:01:09.43
PSV.dial PSR @16:01:09.91 PSR.select :air @16:01:10.04 PS.seek 2.5ml @16:01:10.08 rinseLysateLineINT @16:01:10.10 AFV.select :carrierSolution @16:01:10.20 delay 2 seconds @16:01:12.23 AS.reconfigure ASconfig @16:01:12.28 AS.seek 1ml @16:01:36.16 PRV.dial CBV @16:01:36.19
CBV.dial PRV @16:01:36.20 PSV.dial :mixloop @16:01:37.24 CTV.dial :air,avoiding: :intake @16:01:37.25 PTV.dial :PRVmixing @16:01:37.27 PS.reconfigure PSslow @16:01:37.32 PS.seek 1.2ml @16:02:04.13 PSV.dial :waste1 @16:02:05.48 PS.reconfigure PSconfig @16:02:05.53 PS.seek 0ml @16:02:07.65 delay 1 second @16:02:08.66 AFV.select :holdingCoil @16:02:08.77 delay 1 second @16:02:09.78 ATV.dial AIV @16:02:10.34 AIV.dial :coilToMFB @16:02:10.35 AWV.select :waste @16:02:10.46 AS.reconfigure ASslow0 @16:02:10.51 AS.seek 0ml @16:02:18.59
PSV.dial :air @16:02:19.63 PS.seek 2.5ml @16:02:45.82 PSV.dial :mixloop @16:02:46.29 PS.reconfigure PSslow @16:02:46.34 PS.seek 0ml @16:03:37.44 PSV.dial :air @16:03:37.93 PS.reconfigure PSconfig @16:03:37.98 PS.seek 2.5ml @16:03:51.41 ATV.dial :air2 @16:03:52.06 AS.reconfigure ASslow1 @16:03:52.12 AS.seek 0.05ml @16:04:02.44 ATV.dial :AIV @16:04:03.24 AS.seek 0.01ml @16:04:04.06
PSV.dial :mixloop @16:04:04.66 PS.reconfigure PSslow @16:04:04.71 PS.seek 0ml @16:04:11.67 ATV.dial :waste @16:04:13.20 AS.seek :empty @16:04:15.73 ethanol wash @16:04:15.74 ATV.dial :air2 @16:04:17.46 AS.reconfigure ASslow2 @16:04:17.51 AS.seek 0.009ml @16:04:27.76 delay 3 seconds @16:04:30.79 Pulling 0.4ml of Ethanol Wash @16:04:30.80 ATV.dial :ethanol @16:04:31.58 AS.reconfigure ASslow1 @16:04:31.63 AS.seek 0.409ml @16:04:55.79
Cleaning Collection Syringe @16:04:55.80 CSV.dial CSR @16:04:56.31 Pulling 4ml of CSR.flush @16:04:56.32 CSR.select :flush @16:04:56.45 CS.seek 4ml @16:05:07.64 delay 5 seconds @16:05:12.66 CSR.select :air @16:05:12.80 CS.seek 10ml @16:05:29.05 CSV.dial CTV @16:05:29.08
CTV.dial CSV @16:05:30.50 CBV.dial :waste1 @16:05:30.98 CS.seek 0ml @16:05:52.39 delay 3 seconds @16:05:55.41 ATV.dial :air1 @16:05:55.79 delay 3 seconds @16:05:57.53
CSV.dial CSR @16:05:58.06 Pulling 4ml of CSR.flush @16:05:58.07 CSR.select :flush @16:05:58.20 CS.seek 4ml @16:05:58.87 AS.seek 0.434ml @16:06:04.20 delay 3 seconds @16:06:07.22 ATV.dial :ethanol @16:06:07.82 AS.seek 0.419ml @16:06:09.37
delay 5 seconds @16:06:11.07 delay 3 seconds @16:06:14.09 ATV.dial ASV @16:06:14.39
CSR.select :air @16:06:14.53 CS.seek 10ml @16:06:15.35 AS.seek 0.092ml @16:06:30.90 CSV.dial CTV @16:06:30.93
CTV.dial CSV @16:06:31.43 CBV.dial :waste1 @16:06:31.45 CS.seek 0ml @16:06:57.97 Garaging FlushPuck in Collection Clamp @16:06:58.04 Elbow.reconfigure TightElbow @16:06:58.09 Elbow.seek CC @16:06:58.34 Elbow.reconfigure LooseElbow @16:06:58.45 Forearm.seek CC @16:06:59.45 Hand.close @16:07:00.01 CC.seek :open @16:07:04.37 Forearm.seek :retract @16:07:05.37 Elbow.reconfigure TightElbow @16:07:05.42 Elbow.seek :garage @16:07:08.60 Elbow.reconfigure LooseElbow @16:07:08.71 Forearm.reconfigure TightForearm @16:07:08.76 Forearm.seek :garage @16:07:09.93 Hand.open @16:07:10.58 Forearm.reconfigure LooseForearm @16:07:10.63 Forearm.seek :retract @16:07:11.65 Whole Cell Archive sampling 1500ml @16:07:11.67 Loading wcr puck from tube 2 into Collection Clamp @16:07:11.93 SC.reconfigure SCtight @16:07:11.98 SC.seek 2 @16:07:14.15 SC.reconfigure SCloose @16:07:14.39 Elbow.reconfigure TightElbow @16:07:14.44 Elbow.seek 2 @16:07:17.10 Forearm.reconfigure TightForearm @16:07:17.16 Forearm.seek 2 + 700 counts @16:07:18.24 SE.reconfigure SEpick @16:07:18.33 SE.seek beamBreak @16:07:21.27 SE.configure SEconfig @16:07:21.47 delay 3 seconds @16:07:21.51 Elbow.seek 2 @16:07:21.72 Forearm.seek 2 + 300 counts @16:07:21.92 Forearm.reconfigure LooseForearm @16:07:21.97 Hand.close @16:07:22.66 SE.seek :down @16:07:22.89 Forearm.seek :retract @16:07:23.82 Elbow.seek CC @16:07:24.51 ATV.dial :waste2 @16:07:25.23 AS.reconfigure ASconfig @16:07:25.28 AS.seek :empty @16:07:25.44 SE.coast @16:07:25.48 Elbow.reconfigure LooseElbow @16:07:25.61 Forearm.reconfigure TightForearm @16:07:25.66 Forearm.seek CC @16:07:27.05 CC.seek :closed @16:07:30.99 ATV.dial :air2 @16:07:31.30 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.00 @16:07:31.32 Hand.open @16:07:31.39 AS.reconfigure ASslow1 @16:07:31.45 AS.seek 1ml @16:07:32.08 Forearm.reconfigure LooseForearm @16:07:32.13 Forearm.seek :retract @16:07:33.09 SC.coast @16:07:33.13 collectSample 1500ml w/bubble point of 55psi @16:07:33.15 Priming 7ml @16:07:33.16 Intake.select :close @16:07:33.17 Exhaust.select :close @16:07:33.18 SS.hold @16:07:33.42 Calibrating pressure sensors @16:07:33.43 CTV.dial :air @16:07:34.77 SSV.dial :spit,avoiding: :exhaust @16:07:34.79 SS.reconfigure SSfast @16:07:34.84 SS.seek 5ml @16:07:37.47 SSV.dial CBV @16:07:37.50 CBV.dial SSV @16:07:38.96 delay 3.8 seconds @16:07:42.98 SSV.dial :spit @16:07:44.36 SS.seek 0ml @16:07:46.99 SSV.dial CBV @16:07:48.38 CTV.dial :intake @16:07:49.16 SSV.dial CBV @16:07:49.19 CBV.dial SSV @16:07:49.21 Allowing 40s for 7.0ml of remaining 7.0ml @16:07:49.22 Intake.select :open @16:07:51.97 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:07:51.99 SS.reconfigure SSinhale.with maxSpeed: 150 @16:07:52.04 SS.seek 7ml @16:07:55.58 Vacuum too high -- waiting up to 40s @16:07:57.98 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:07:58.00 SS.reconfigure SSinhale.with maxSpeed: 135 @16:07:58.05 SS.seek 7ml @16:08:01.29 Stroke completed -- waiting for pressure to equalize ... @16:08:05.79 Intake.select :close @16:08:05.96 SSV.dial :exhaust @16:08:06.78 Exhaust.select :open @16:08:06.89 SS.reconfigure SSexhale @16:08:06.95 SS.seek 0ml @16:08:10.60 Exhaust.select :close @16:08:10.72 Sampling 1500.0ml @16:08:10.74 Intake.select :close @16:08:10.75 Exhaust.select :close @16:08:10.77 SS.reconfigure SSconfig @16:08:10.81 SS.hold @16:08:11.04 CTV.dial :intake @16:08:11.06 SSV.dial CBV @16:08:11.09 CBV.dial SSV @16:08:11.90 Allowing 130s for 25.0ml of remaining 1500.0ml @16:08:11.91 Intake.select :open @16:08:14.09 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:08:14.11 SS.reconfigure SSinhale.with maxSpeed: 135 @16:08:14.16 SS.seek 25ml @16:08:18.26 Vacuum too high -- waiting up to 40s @16:08:20.15 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:08:20.17 SS.reconfigure SSinhale.with maxSpeed: 122 @16:08:20.22 SS.seek 25ml @16:08:22.96 Vacuum too high -- waiting up to 40s @16:08:24.59 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:08:24.61 SS.reconfigure SSinhale.with maxSpeed: 110 @16:08:24.66 SS.seek 25ml @16:08:27.37 Vacuum too high -- waiting up to 40s @16:08:29.06 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:08:29.08 SS.reconfigure SSinhale.with maxSpeed: 99 @16:08:29.13 SS.seek 25ml @16:08:32.17 Vacuum too high -- waiting up to 40s @16:08:33.54 Intake @33.5psia, vacuum from 20.0 to 24.0psi @16:08:33.56 SS.reconfigure SSinhale.with maxSpeed: 89 @16:08:33.61 SS.seek 25ml @16:08:36.65 Vacuum too high -- waiting up to 40s @16:08:38.03 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:08:38.05 SS.reconfigure SSinhale.with maxSpeed: 80 @16:08:38.10 SS.seek 25ml @16:08:41.14 Vacuum too high -- waiting up to 40s @16:08:42.53 Intake @33.5psia, vacuum from 20.0 to 24.0psi @16:08:42.55 SS.reconfigure SSinhale.with maxSpeed: 72 @16:08:42.60 SS.seek 25ml @16:08:46.55 Vacuum too high -- waiting up to 40s @16:08:47.66 Intake @33.4psia, vacuum from 20.0 to 24.0psi @16:08:47.68 SS.reconfigure SSinhale.with maxSpeed: 65 @16:08:47.73 SS.seek 25ml @16:08:51.27 Stroke completed -- waiting for pressure to equalize ... @16:08:54.66 Intake.select :close @16:08:54.82 SSV.dial :exhaust @16:08:55.61 Exhaust.select :open @16:08:55.72 SS.reconfigure SSexhale @16:08:55.77 SS.seek 0ml @16:09:04.82 Exhaust.select :close @16:09:04.94 SSV.dial CBV @16:09:04.97 CBV.dial SSV @16:09:05.80 Allowing 130s for 25.0ml of remaining 1475.0ml @16:09:05.81 Intake.select :open @16:09:07.99 Intake @33.6psia, vacuum from 20.0 to 24.0psi @16:09:08.01 SS.reconfigure SSinhale.with maxSpeed: 65 @16:09:08.06 SS.seek 25ml @16:09:14.51 Vacuum too high -- waiting up to 40s @16:09:15.62 Intake @33.4psia, vacuum from 20.0 to 24.0psi @16:09:15.65 SS.reconfigure SSinhale.with maxSpeed: 59 @16:09:15.70 SS.seek 25ml @16:09:24.67 Vacuum too high -- waiting up to 40s @16:09:26.10 Intake @33.5psia, vacuum from 20.0 to 24.0psi @16:09:26.12 SS.reconfigure SSinhale.with maxSpeed: 53 @16:09:26.17 SS.seek 25ml @16:09:43.18 Stroke completed -- waiting for pressure to equalize ... @16:09:46.42 Intake.select :close @16:09:46.59 SSV.dial :exhaust @16:09:47.41 Exhaust.select :open @16:09:47.52 SS.reconfigure SSexhale @16:09:47.58 SS.seek 0ml @16:09:56.52 Exhaust.select :close @16:09:56.63 SSV.dial CBV @16:09:56.66 CBV.dial SSV @16:09:57.47 Allowing 130s for 25.0ml of remaining 1450.0ml @16:09:57.48 Intake.select :open @16:09:59.67 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:09:59.69 SS.reconfigure SSinhale.with maxSpeed: 53 @16:09:59.74 SS.seek 25ml @16:10:33.52 Stroke completed -- waiting for pressure to equalize ... @16:10:36.75 Intake.select :close @16:10:36.91 SSV.dial :exhaust @16:10:37.72 Exhaust.select :open @16:10:37.88 SS.reconfigure SSexhale @16:10:37.94 SS.seek 0ml @16:10:46.93 Exhaust.select :close @16:10:47.05 SSV.dial CBV @16:10:47.08 CBV.dial SSV @16:10:47.91 Allowing 130s for 25.0ml of remaining 1425.0ml @16:10:47.92 Intake.select :open @16:10:50.11 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:10:50.13 SS.reconfigure SSinhale.with maxSpeed: 53 @16:10:50.18 SS.seek 25ml @16:10:53.03 delay 5 seconds @16:10:58.05 ATV.dial ASV @16:10:58.98 ASV.dial :SPEdelivery @16:10:59.00 AS.seek 0.02ml @16:11:18.95 Vacuum too high -- waiting up to 40s @16:11:20.07 Intake @33.2psia, vacuum from 20.0 to 24.0psi @16:11:20.09 SS.reconfigure SSinhale.with maxSpeed: 48 @16:11:20.14 SS.seek 25ml @16:11:26.01 Stroke completed -- waiting for pressure to equalize ... @16:11:28.64 Intake.select :close @16:11:28.80 SSV.dial :exhaust @16:11:29.61 Exhaust.select :open @16:11:29.72 SS.reconfigure SSexhale @16:11:29.78 SS.seek 0ml @16:11:38.77 Exhaust.select :close @16:11:38.89 SSV.dial CBV @16:11:38.92 CBV.dial SSV @16:11:39.72 Allowing 130s for 25.0ml of remaining 1400.0ml @16:11:39.73 Intake.select :open @16:11:41.92 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:11:41.94 SS.reconfigure SSinhale.with maxSpeed: 48 @16:11:41.99 SS.seek 25ml @16:12:19.01 Stroke completed -- waiting for pressure to equalize ... @16:12:22.19 Intake.select :close @16:12:22.36 SSV.dial :exhaust @16:12:23.17 Exhaust.select :open @16:12:23.28 SS.reconfigure SSexhale @16:12:23.34 SS.seek 0ml @16:12:32.26 Exhaust.select :close @16:12:32.38 SSV.dial CBV @16:12:32.41 CBV.dial SSV @16:12:33.24 Allowing 130s for 25.0ml of remaining 1375.0ml @16:12:33.25 Intake.select :open @16:12:35.44 Intake @33.6psia, vacuum from 20.0 to 24.0psi @16:12:35.46 SS.reconfigure SSinhale.with maxSpeed: 48 @16:12:35.51 SS.seek 25ml @16:13:12.57 Stroke completed -- waiting for pressure to equalize ... @16:13:15.80 Intake.select :close @16:13:15.97 SSV.dial :exhaust @16:13:16.78 Exhaust.select :open @16:13:16.89 SS.reconfigure SSexhale @16:13:16.94 SS.seek 0ml @16:13:25.62 Exhaust.select :close @16:13:25.74 SSV.dial CBV @16:13:25.77 CBV.dial SSV @16:13:26.58 Allowing 130s for 25.0ml of remaining 1350.0ml @16:13:26.59 Intake.select :open @16:13:28.77 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:13:28.79 SS.reconfigure SSinhale.with maxSpeed: 48 @16:13:28.84 SS.seek 25ml @16:14:06.09 Stroke completed -- waiting for pressure to equalize ... @16:14:09.33 Intake.select :close @16:14:09.49 SSV.dial :exhaust @16:14:10.30 Exhaust.select :open @16:14:10.41 SS.reconfigure SSexhale @16:14:10.46 SS.seek 0ml @16:14:16.36 delay 5 seconds @16:14:19.59 Exhaust.select :close @16:14:19.71 SSV.dial CBV @16:14:19.76 CBV.dial SSV @16:14:20.55 Allowing 130s for 25.0ml of remaining 1325.0ml @16:14:20.56 Intake.select :open @16:14:21.37 ATV.dial :air2 @16:14:22.38 AS.seek 0.52ml @16:14:22.75 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:14:22.77 SS.reconfigure SSinhale.with maxSpeed: 48 @16:14:22.82 SS.seek 25ml @16:15:00.07 Stroke completed -- waiting for pressure to equalize ... @16:15:03.30 Intake.select :close @16:15:03.46 SSV.dial :exhaust @16:15:04.27 Exhaust.select :open @16:15:04.38 SS.reconfigure SSexhale @16:15:04.43 SS.seek 0ml @16:15:13.23 Exhaust.select :close @16:15:13.35 SSV.dial CBV @16:15:13.38 CBV.dial SSV @16:15:14.19 Allowing 130s for 25.0ml of remaining 1300.0ml @16:15:14.20 Intake.select :open @16:15:16.85 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:15:16.96 SS.reconfigure SSinhale.with maxSpeed: 48 @16:15:17.01 SS.seek 25ml @16:15:54.68 Stroke completed -- waiting for pressure to equalize ... @16:15:57.93 Intake.select :close @16:15:58.10 SSV.dial :exhaust @16:15:58.91 Exhaust.select :open @16:15:59.02 SS.reconfigure SSexhale @16:15:59.08 SS.seek 0ml @16:16:03.25 delay 5 seconds @16:16:08.18 Exhaust.select :close @16:16:08.26 ATV.dial ASV @16:16:08.33 SSV.dial CBV @16:16:08.36 CBV.dial SSV @16:16:09.17 Allowing 130s for 25.0ml of remaining 1275.0ml @16:16:09.19 Intake.select :open @16:16:09.26 ASV.dial :wasteR @16:16:09.63 AS.seek 0.3ml @16:16:11.39 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:16:11.41 SS.reconfigure SSinhale.with maxSpeed: 48 @16:16:11.46 SS.seek 25ml @16:16:48.44 Stroke completed -- waiting for pressure to equalize ... @16:16:52.32 Intake.select :close @16:16:52.49 SSV.dial :exhaust @16:16:53.30 Exhaust.select :open @16:16:53.42 SS.reconfigure SSexhale @16:16:53.48 SS.seek 0ml @16:16:54.16 delay 20 seconds @16:17:02.45 Exhaust.select :close @16:17:02.57 SSV.dial CBV @16:17:02.60 CBV.dial SSV @16:17:03.41 Allowing 130s for 25.0ml of remaining 1250.0ml @16:17:03.42 Intake.select :open @16:17:05.61 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:17:05.63 SS.reconfigure SSinhale.with maxSpeed: 48 @16:17:05.68 SS.seek 25ml @16:17:14.19 AS.seek 0.07ml @16:17:43.13 Stroke completed -- waiting for pressure to equalize ... @16:17:46.38 Intake.select :close @16:17:46.54 SSV.dial :exhaust @16:17:47.35 Exhaust.select :open @16:17:47.46 SS.reconfigure SSexhale @16:17:47.51 SS.seek 0ml @16:17:56.40 Exhaust.select :close @16:17:56.52 SSV.dial CBV @16:17:56.55 CBV.dial SSV @16:17:57.38 Allowing 130s for 25.0ml of remaining 1225.0ml @16:17:57.39 Intake.select :open @16:17:59.58 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:17:59.60 SS.reconfigure SSinhale.with maxSpeed: 48 @16:17:59.65 SS.seek 25ml @16:18:00.75 delay 20 seconds @16:18:20.77 ATV.dial :waste2 @16:18:21.48 AS.reconfigure ASconfig @16:18:21.53 AS.seek :empty @16:18:25.95 Elution @16:18:25.96 ATV.dial :air1 @16:18:27.01 AS.reconfigure ASslow1 @16:18:27.06 AS.seek 0.049ml @16:18:36.82 Stroke completed -- waiting for pressure to equalize ... @16:18:38.05 delay 3 seconds @16:18:40.63 Intake.select :close @16:18:40.79 SSV.dial :exhaust @16:18:41.06 ATV.dial :ElutionWater @16:18:41.60 Exhaust.select :open @16:18:41.75 AS.seek 0.109ml @16:18:41.81 SS.reconfigure SSexhale @16:18:41.88 SS.seek 0ml @16:18:50.63 Exhaust.select :close @16:18:50.75 SSV.dial CBV @16:18:50.78 CBV.dial SSV @16:18:51.60 Allowing 130s for 25.0ml of remaining 1200.0ml @16:18:51.61 Intake.select :open @16:18:53.79 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:18:53.81 SS.reconfigure SSinhale.with maxSpeed: 48 @16:18:53.86 SS.seek 25ml @16:18:54.22 delay 3 seconds @16:18:57.24 ATV.dial ASV,via: :air2 @16:18:59.16 ASV.dial :SPEelution @16:18:59.72 AFV.select :carrierSolution @16:18:59.88 AS.reconfigure ASnorm @16:18:59.93 AS.seek 0.759ml @16:19:31.34 Stroke completed -- waiting for pressure to equalize ... @16:19:32.99 delay 3 seconds @16:19:35.21 Intake.select :close @16:19:35.37 SSV.dial :exhaust @16:19:36.01 AFV.select :holdingCoil @16:19:36.19 Exhaust.select :open @16:19:36.23 AS.reconfigure ASslow1 @16:19:36.32 AS.seek 0.474ml @16:19:36.38 SS.reconfigure SSexhale @16:19:36.43 SS.seek 0ml @16:19:45.43 Exhaust.select :close @16:19:45.55 SSV.dial CBV @16:19:45.58 CBV.dial SSV @16:19:46.41 Allowing 130s for 25.0ml of remaining 1175.0ml @16:19:46.42 Intake.select :open @16:19:48.61 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:19:48.63 SS.reconfigure SSinhale.with maxSpeed: 48 @16:19:48.68 SS.seek 25ml @16:20:21.73 Vacuum too high -- waiting up to 40s @16:20:22.83 Intake @33.5psia, vacuum from 20.0 to 24.0psi @16:20:22.86 SS.reconfigure SSinhale.with maxSpeed: 43 @16:20:22.91 SS.seek 25ml @16:20:27.65 Stroke completed -- waiting for pressure to equalize ... @16:20:30.89 Intake.select :close @16:20:31.05 SSV.dial :exhaust @16:20:31.86 Exhaust.select :open @16:20:31.97 SS.reconfigure SSexhale @16:20:32.02 SS.seek 0ml @16:20:33.94 delay 5 seconds @16:20:39.02 AS.reconfigure ASslow2 @16:20:39.07 AS.seek 0.439ml @16:20:41.15 Exhaust.select :close @16:20:41.84 SSV.dial CBV @16:20:41.87 CBV.dial SSV @16:20:42.67 Allowing 130s for 25.0ml of remaining 1150.0ml @16:20:42.68 Intake.select :open @16:20:44.87 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:20:44.89 SS.reconfigure SSinhale.with maxSpeed: 43 @16:20:44.95 SS.seek 25ml @16:21:14.47 delay 1:00 @16:21:25.77 Stroke completed -- waiting for pressure to equalize ... @16:21:29.00 Intake.select :close @16:21:29.16 SSV.dial :exhaust @16:21:29.97 Exhaust.select :open @16:21:30.09 SS.reconfigure SSexhale @16:21:30.14 SS.seek 0ml @16:21:39.07 Exhaust.select :close @16:21:39.19 SSV.dial CBV @16:21:39.22 CBV.dial SSV @16:21:40.05 Allowing 130s for 25.0ml of remaining 1125.0ml @16:21:40.06 Intake.select :open @16:21:42.25 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:21:42.27 SS.reconfigure SSinhale.with maxSpeed: 43 @16:21:42.32 SS.seek 25ml @16:22:14.50 pushing eluate to holding coil @16:22:14.57 AS.seek 0.279ml @16:22:23.65 Stroke completed -- waiting for pressure to equalize ... @16:22:26.88 Intake.select :close @16:22:27.05 SSV.dial :exhaust @16:22:27.86 Exhaust.select :open @16:22:27.97 SS.reconfigure SSexhale @16:22:28.03 SS.seek 0ml @16:22:36.84 Exhaust.select :close @16:22:36.96 SSV.dial CBV @16:22:36.99 CBV.dial SSV @16:22:37.80 Allowing 130s for 25.0ml of remaining 1100.0ml @16:22:37.81 Intake.select :open @16:22:39.99 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:22:40.01 SS.reconfigure SSinhale.with maxSpeed: 43 @16:22:40.06 SS.seek 25ml @16:23:21.69 Stroke completed -- waiting for pressure to equalize ... @16:23:25.56 Intake.select :close @16:23:25.72 SSV.dial :exhaust @16:23:26.53 Exhaust.select :open @16:23:26.64 SS.reconfigure SSexhale @16:23:26.69 SS.seek 0ml @16:23:35.71 Exhaust.select :close @16:23:35.83 SSV.dial CBV @16:23:35.86 CBV.dial SSV @16:23:36.69 Allowing 130s for 25.0ml of remaining 1075.0ml @16:23:36.70 Intake.select :open @16:23:38.88 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:23:38.90 SS.reconfigure SSinhale.with maxSpeed: 43 @16:23:38.95 SS.seek 25ml @16:24:20.01 Stroke completed -- waiting for pressure to equalize ... @16:24:24.45 Intake.select :close @16:24:24.61 SSV.dial :exhaust @16:24:25.45 Exhaust.select :open @16:24:25.57 SS.reconfigure SSexhale @16:24:25.63 SS.seek 0ml @16:24:34.59 Exhaust.select :close @16:24:34.71 SSV.dial CBV @16:24:34.74 CBV.dial SSV @16:24:35.55 Allowing 130s for 25.0ml of remaining 1050.0ml @16:24:35.56 Intake.select :open @16:24:37.75 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:24:37.77 SS.reconfigure SSinhale.with maxSpeed: 43 @16:24:37.82 SS.seek 25ml @16:24:55.64 delay 30 seconds @16:25:19.12 Stroke completed -- waiting for pressure to equalize ... @16:25:23.01 Intake.select :close @16:25:23.17 SSV.dial :exhaust @16:25:23.99 Exhaust.select :open @16:25:24.10 SS.reconfigure SSexhale @16:25:24.17 SS.seek 0ml @16:25:25.67 ATV.dial :waste,via: :air3 @16:25:27.25 AS.reconfigure ASconfig @16:25:27.30 AS.seek :empty @16:25:33.16 Exhaust.select :close @16:25:33.28 SSV.dial CBV @16:25:33.31 CBV.dial SSV @16:25:34.13 Allowing 130s for 25.0ml of remaining 1025.0ml @16:25:34.14 Intake.select :open @16:25:36.32 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:25:36.34 SS.reconfigure SSinhale.with maxSpeed: 43 @16:25:36.39 SS.seek 25ml @16:25:43.70 Parking Bleach in LysateSource line and SPEcolumn @16:25:43.71 ATV.dial :air3 @16:25:44.60 AS.reconfigure ASslow1 @16:25:44.65 AS.seek 0.029ml @16:25:51.01 ATV.dial :ElutionWater @16:25:51.60 AS.seek 0.059ml @16:25:57.90 ATV.dial :air3 @16:25:58.51 AS.seek 0.069ml @16:26:00.78 delay 2 seconds @16:26:02.79 ATV.dial :bleach @16:26:03.24 AS.seek 0.769ml @16:26:17.73 Stroke completed -- waiting for pressure to equalize ... @16:26:21.62 Intake.select :close @16:26:21.78 SSV.dial :exhaust @16:26:22.60 Exhaust.select :open @16:26:22.71 SS.reconfigure SSexhale @16:26:22.78 SS.seek 0ml @16:26:31.79 Exhaust.select :close @16:26:31.91 SSV.dial CBV @16:26:31.94 CBV.dial SSV @16:26:32.75 Allowing 130s for 25.0ml of remaining 1000.0ml @16:26:32.76 Intake.select :open @16:26:34.94 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:26:34.97 SS.reconfigure SSinhale.with maxSpeed: 43 @16:26:35.01 SS.seek 25ml @16:27:16.35 Stroke completed -- waiting for pressure to equalize ... @16:27:20.16 Intake.select :close @16:27:20.33 SSV.dial :exhaust @16:27:21.14 Exhaust.select :open @16:27:21.25 SS.reconfigure SSexhale @16:27:21.31 SS.seek 0ml @16:27:30.43 Exhaust.select :close @16:27:30.55 SSV.dial CBV @16:27:30.58 CBV.dial SSV @16:27:31.41 Allowing 130s for 25.0ml of remaining 975.0ml @16:27:31.42 Intake.select :open @16:27:33.61 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:27:33.63 SS.reconfigure SSinhale.with maxSpeed: 43 @16:27:33.68 SS.seek 25ml @16:28:15.04 Stroke completed -- waiting for pressure to equalize ... @16:28:19.54 Intake.select :close @16:28:19.71 SSV.dial :exhaust @16:28:20.53 Exhaust.select :open @16:28:20.65 SS.reconfigure SSexhale @16:28:20.71 SS.seek 0ml @16:28:24.27 delay 3 seconds @16:28:27.29 ATV.dial :air3 @16:28:27.81 delay 3 seconds @16:28:29.50 Exhaust.select :close @16:28:29.61 SSV.dial CBV @16:28:29.64 CBV.dial SSV @16:28:30.45 Allowing 130s for 25.0ml of remaining 950.0ml @16:28:30.46 Intake.select :open @16:28:30.89 AS.seek 0.794ml @16:28:32.65 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:28:32.67 SS.reconfigure SSinhale.with maxSpeed: 43 @16:28:32.72 SS.seek 25ml @16:28:36.21 delay 3 seconds @16:28:39.23 ATV.dial :bleach @16:28:39.84 AS.seek 0.779ml @16:28:43.10 delay 3 seconds @16:28:46.12 ATV.dial :AIV @16:28:47.28 AS.reconfigure ASconfig @16:28:47.33 AS.seek 0.015ml @16:29:14.38 Stroke completed -- waiting for pressure to equalize ... @16:29:18.91 Intake.select :close @16:29:19.07 SSV.dial :exhaust @16:29:19.89 Exhaust.select :open @16:29:20.01 SS.reconfigure SSexhale @16:29:20.07 SS.seek 0ml @16:29:28.88 Exhaust.select :close @16:29:29.00 SSV.dial CBV @16:29:29.03 CBV.dial SSV @16:29:29.84 Allowing 130s for 25.0ml of remaining 925.0ml @16:29:29.85 Intake.select :open @16:29:31.52 ATV.dial :waste @16:29:32.03 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:29:32.05 SS.reconfigure SSinhale.with maxSpeed: 43 @16:29:32.10 SS.seek 25ml @16:29:32.89 AS.seek :empty @16:29:34.10 ATV.dial :air3 @16:29:34.74 AS.reconfigure ASslow1 @16:29:34.79 AS.seek 0.009ml @16:29:37.20 delay 2 seconds @16:29:39.21 ATV.dial :bleach @16:29:39.68 AS.seek 0.579ml @16:30:08.34 Vacuum too high -- waiting up to 40s @16:30:09.70 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:30:09.72 SS.reconfigure SSinhale.with maxSpeed: 39 @16:30:09.77 SS.seek 25ml @16:30:16.02 Stroke completed -- waiting for pressure to equalize ... @16:30:19.26 Intake.select :close @16:30:19.42 SSV.dial :exhaust @16:30:20.24 Exhaust.select :open @16:30:20.35 SS.reconfigure SSexhale @16:30:20.41 SS.seek 0ml @16:30:29.72 Exhaust.select :close @16:30:29.83 SSV.dial CBV @16:30:29.86 CBV.dial SSV @16:30:30.67 Allowing 130s for 25.0ml of remaining 900.0ml @16:30:30.68 Intake.select :open @16:30:32.87 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:30:32.89 SS.reconfigure SSinhale.with maxSpeed: 39 @16:30:32.94 SS.seek 25ml @16:31:18.27 Stroke completed -- waiting for pressure to equalize ... @16:31:22.14 Intake.select :close @16:31:22.31 SSV.dial :exhaust @16:31:23.13 Exhaust.select :open @16:31:23.24 SS.reconfigure SSexhale @16:31:23.30 SS.seek 0ml @16:31:32.37 Exhaust.select :close @16:31:32.49 SSV.dial CBV @16:31:32.52 CBV.dial SSV @16:31:33.34 Allowing 130s for 25.0ml of remaining 875.0ml @16:31:33.35 Intake.select :open @16:31:34.63 delay 3 seconds @16:31:35.54 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:31:35.56 SS.reconfigure SSinhale.with maxSpeed: 39 @16:31:35.61 SS.seek 25ml @16:31:37.65 ATV.dial :air3 @16:31:38.19 delay 3 seconds @16:31:41.28 AS.seek 0.604ml @16:31:46.58 delay 3 seconds @16:31:49.59 ATV.dial :bleach @16:31:50.20 AS.seek 0.589ml @16:31:53.49 delay 3 seconds @16:31:56.51 ATV.dial :ASV @16:31:57.47 ASV.dial :wasteR @16:31:58.03 AS.seek 0ml @16:32:21.07 Stroke completed -- waiting for pressure to equalize ... @16:32:24.95 Intake.select :close @16:32:25.12 SSV.dial :exhaust @16:32:25.93 Exhaust.select :open @16:32:26.05 SS.reconfigure SSexhale @16:32:26.12 SS.seek 0ml @16:32:35.29 Exhaust.select :close @16:32:35.41 SSV.dial CBV @16:32:35.44 CBV.dial SSV @16:32:36.25 Allowing 130s for 25.0ml of remaining 850.0ml @16:32:36.26 Intake.select :open @16:32:38.45 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:32:38.47 SS.reconfigure SSinhale.with maxSpeed: 39 @16:32:38.52 SS.seek 25ml @16:33:23.58 Stroke completed -- waiting for pressure to equalize ... @16:33:27.54 Intake.select :close @16:33:27.70 SSV.dial :exhaust @16:33:28.52 Exhaust.select :open @16:33:28.63 SS.reconfigure SSexhale @16:33:28.70 SS.seek 0ml @16:33:37.76 Exhaust.select :close @16:33:37.88 SSV.dial CBV @16:33:37.91 CBV.dial SSV @16:33:38.74 Allowing 130s for 25.0ml of remaining 825.0ml @16:33:38.75 Intake.select :open @16:33:40.94 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:33:40.96 SS.reconfigure SSinhale.with maxSpeed: 39 @16:33:41.01 SS.seek 25ml @16:33:56.79 delay 1:00 @16:34:26.35 Stroke completed -- waiting for pressure to equalize ... @16:34:30.87 Intake.select :close @16:34:31.03 SSV.dial :exhaust @16:34:31.85 Exhaust.select :open @16:34:31.96 SS.reconfigure SSexhale @16:34:32.02 SS.seek 0ml @16:34:41.13 Exhaust.select :close @16:34:41.25 SSV.dial CBV @16:34:41.28 CBV.dial SSV @16:34:42.08 Allowing 130s for 25.0ml of remaining 800.0ml @16:34:42.09 Intake.select :open @16:34:44.28 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:34:44.30 SS.reconfigure SSinhale.with maxSpeed: 39 @16:34:44.35 SS.seek 25ml @16:34:56.81 AFV.select :carrierSolution @16:34:56.92 delay 2 seconds @16:34:58.95 AS.reconfigure ASconfig @16:34:59.00 AS.seek 1ml @16:35:29.66 Stroke completed -- waiting for pressure to equalize ... @16:35:34.18 Intake.select :close @16:35:34.34 SSV.dial :exhaust @16:35:35.16 Exhaust.select :open @16:35:35.27 SS.reconfigure SSexhale @16:35:35.32 SS.seek 0ml @16:35:44.58 Exhaust.select :close @16:35:44.70 SSV.dial CBV @16:35:44.73 CBV.dial SSV @16:35:45.55 Allowing 130s for 25.0ml of remaining 775.0ml @16:35:45.56 Intake.select :open @16:35:48.22 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:35:48.33 SS.reconfigure SSinhale.with maxSpeed: 39 @16:35:48.38 SS.seek 25ml @16:35:56.77 delay 1 second @16:35:57.78 AFV.select :holdingCoil @16:35:57.89 delay 1 second @16:35:58.92 ATV.dial ASV @16:35:58.93 ASV.dial :wasteR @16:35:58.95 AS.reconfigure ASslow1 @16:35:58.99 AS.seek 0ml @16:36:33.79 Stroke completed -- waiting for pressure to equalize ... @16:36:38.31 Intake.select :close @16:36:38.47 SSV.dial :exhaust @16:36:39.28 Exhaust.select :open @16:36:39.40 SS.reconfigure SSexhale @16:36:39.46 SS.seek 0ml @16:36:48.58 Exhaust.select :close @16:36:48.70 SSV.dial CBV @16:36:48.73 CBV.dial SSV @16:36:49.53 Allowing 130s for 25.0ml of remaining 750.0ml @16:36:49.54 Intake.select :open @16:36:51.73 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:36:51.75 SS.reconfigure SSinhale.with maxSpeed: 39 @16:36:51.80 SS.seek 25ml @16:37:37.13 Stroke completed -- waiting for pressure to equalize ... @16:37:41.62 Intake.select :close @16:37:41.78 SSV.dial :exhaust @16:37:42.60 Exhaust.select :open @16:37:42.71 SS.reconfigure SSexhale @16:37:42.76 SS.seek 0ml @16:37:51.87 Exhaust.select :close @16:37:51.99 SSV.dial CBV @16:37:52.02 CBV.dial SSV @16:37:52.83 Allowing 130s for 25.0ml of remaining 725.0ml @16:37:52.84 Intake.select :open @16:37:55.03 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:37:55.05 SS.reconfigure SSinhale.with maxSpeed: 39 @16:37:55.10 SS.seek 25ml @16:38:35.26 Vacuum too high -- waiting up to 40s @16:38:36.63 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:38:36.66 SS.reconfigure SSinhale.with maxSpeed: 35 @16:38:36.71 SS.seek 25ml @16:38:42.57 Stroke completed -- waiting for pressure to equalize ... @16:38:46.44 Intake.select :close @16:38:46.60 SSV.dial :exhaust @16:38:47.42 Exhaust.select :open @16:38:47.53 SS.reconfigure SSexhale @16:38:47.60 SS.seek 0ml @16:38:57.04 Exhaust.select :close @16:38:57.73 SSV.dial CBV @16:38:57.76 CBV.dial SSV @16:38:58.58 Allowing 130s for 25.0ml of remaining 700.0ml @16:38:58.59 Intake.select :open @16:39:00.78 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:39:00.80 SS.reconfigure SSinhale.with maxSpeed: 35 @16:39:00.85 SS.seek 25ml @16:39:20.38 delay 20 seconds @16:39:40.40 ATV.dial :air3 @16:39:41.81 AS.seek 0.59ml @16:39:51.27 Stroke completed -- waiting for pressure to equalize ... @16:39:55.14 Intake.select :close @16:39:55.30 SSV.dial :exhaust @16:39:56.11 Exhaust.select :open @16:39:56.22 SS.reconfigure SSexhale @16:39:56.27 SS.seek 0ml @16:40:05.32 Exhaust.select :close @16:40:05.44 SSV.dial CBV @16:40:05.47 CBV.dial SSV @16:40:06.27 Allowing 130s for 25.0ml of remaining 675.0ml @16:40:06.28 Intake.select :open @16:40:08.47 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:40:08.49 SS.reconfigure SSinhale.with maxSpeed: 35 @16:40:08.54 SS.seek 25ml @16:40:58.94 Stroke completed -- waiting for pressure to equalize ... @16:41:03.46 Intake.select :close @16:41:03.62 SSV.dial :exhaust @16:41:04.42 Exhaust.select :open @16:41:04.53 SS.reconfigure SSexhale @16:41:04.58 SS.seek 0ml @16:41:13.91 Exhaust.select :close @16:41:14.03 SSV.dial CBV @16:41:14.06 CBV.dial SSV @16:41:14.88 Allowing 130s for 25.0ml of remaining 650.0ml @16:41:14.89 Intake.select :open @16:41:17.07 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:41:17.10 SS.reconfigure SSinhale.with maxSpeed: 35 @16:41:17.14 SS.seek 25ml @16:41:40.72 delay 3 seconds @16:41:43.74 ATV.dial :ASV @16:41:45.04 ASV.dial :wasteR @16:41:45.13 AS.seek 0.09ml @16:42:07.55 Stroke completed -- waiting for pressure to equalize ... @16:42:12.06 Intake.select :close @16:42:12.22 SSV.dial :exhaust @16:42:13.03 Exhaust.select :open @16:42:13.14 SS.reconfigure SSexhale @16:42:13.19 SS.seek 0ml @16:42:22.44 Exhaust.select :close @16:42:22.56 SSV.dial CBV @16:42:22.61 CBV.dial SSV @16:42:23.38 Allowing 130s for 25.0ml of remaining 625.0ml @16:42:23.39 Intake.select :open @16:42:26.15 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:42:26.17 SS.reconfigure SSinhale.with maxSpeed: 35 @16:42:26.22 SS.seek 25ml @16:43:16.62 Stroke completed -- waiting for pressure to equalize ... @16:43:21.14 Intake.select :close @16:43:21.30 SSV.dial :exhaust @16:43:22.11 Exhaust.select :open @16:43:22.22 SS.reconfigure SSexhale @16:43:22.28 SS.seek 0ml @16:43:26.01 delay 30 seconds @16:43:31.12 Exhaust.select :close @16:43:31.24 SSV.dial CBV @16:43:31.27 CBV.dial SSV @16:43:32.09 Allowing 130s for 25.0ml of remaining 600.0ml @16:43:32.10 Intake.select :open @16:43:34.29 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:43:34.31 SS.reconfigure SSinhale.with maxSpeed: 35 @16:43:34.36 SS.seek 25ml @16:43:56.03 ATV.dial :extSrc @16:43:56.77 delay 3 seconds @16:43:59.79 ATV.dial :ElutionWater @16:44:00.84 AS.seek 0.12ml @16:44:07.14 ATV.dial :air3 @16:44:07.75 AS.seek 0.13ml @16:44:10.02 delay 2 seconds @16:44:12.03 ATV.dial :bleach @16:44:12.49 AS.seek 0.23ml @16:44:25.08 Stroke completed -- waiting for pressure to equalize ... @16:44:30.23 Intake.select :close @16:44:30.39 SSV.dial :exhaust @16:44:31.20 Exhaust.select :open @16:44:31.31 SS.reconfigure SSexhale @16:44:31.36 SS.seek 0ml @16:44:32.85 delay 3 seconds @16:44:35.86 ATV.dial :air3 @16:44:36.40 delay 3 seconds @16:44:39.49 AS.seek 0.255ml @16:44:40.67 Exhaust.select :close @16:44:40.78 SSV.dial CBV @16:44:40.81 CBV.dial SSV @16:44:41.63 Allowing 130s for 25.0ml of remaining 575.0ml @16:44:41.64 Intake.select :open @16:44:43.82 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:44:43.84 SS.reconfigure SSinhale.with maxSpeed: 35 @16:44:43.89 SS.seek 25ml @16:44:44.76 delay 3 seconds @16:44:47.77 ATV.dial :bleach @16:44:48.37 AS.seek 0.24ml @16:44:51.68 delay 3 seconds @16:44:54.70 ATV.dial :ASV @16:44:55.66 ASV.dial :SPEdelivery @16:44:56.01 AFV.select :carrierSolution @16:44:56.13 delay 2 seconds @16:44:58.16 AS.reconfigure ASconfig @16:44:58.21 AS.seek 0.5ml @16:45:13.46 delay 1 second @16:45:14.47 AFV.select :holdingCoil @16:45:14.63 AS.reconfigure ASslow1 @16:45:14.68 AS.seek 0.17ml @16:45:34.57 Stroke completed -- waiting for pressure to equalize ... @16:45:39.67 Intake.select :close @16:45:39.84 SSV.dial :exhaust @16:45:40.64 Exhaust.select :open @16:45:40.76 SS.reconfigure SSexhale @16:45:40.82 SS.seek 0ml @16:45:49.97 Exhaust.select :close @16:45:50.09 SSV.dial CBV @16:45:50.12 CBV.dial SSV @16:45:50.94 Allowing 130s for 25.0ml of remaining 550.0ml @16:45:50.95 Intake.select :open @16:45:53.14 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:45:53.16 SS.reconfigure SSinhale.with maxSpeed: 35 @16:45:53.21 SS.seek 25ml @16:46:21.36 SPE.hold 2:46:40,95C @16:46:21.42 delay 3:00 @16:46:43.48 Stroke completed -- waiting for pressure to equalize ... @16:46:48.63 Intake.select :close @16:46:48.79 SSV.dial :exhaust @16:46:49.61 Exhaust.select :open @16:46:49.72 SS.reconfigure SSexhale @16:46:49.78 SS.seek 0ml @16:46:59.18 Exhaust.select :close @16:46:59.30 SSV.dial CBV @16:46:59.33 CBV.dial SSV @16:47:00.19 Allowing 130s for 25.0ml of remaining 525.0ml @16:47:00.20 Intake.select :open @16:47:02.39 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:47:02.41 SS.reconfigure SSinhale.with maxSpeed: 35 @16:47:02.46 SS.seek 25ml @16:47:44.04 Vacuum too high -- waiting up to 40s @16:47:45.41 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:47:45.43 SS.reconfigure SSinhale.with maxSpeed: 32 @16:47:45.48 SS.seek 25ml @16:47:54.97 Stroke completed -- waiting for pressure to equalize ... @16:47:59.49 Intake.select :close @16:47:59.66 SSV.dial :exhaust @16:48:00.49 Exhaust.select :open @16:48:00.64 SS.reconfigure SSexhale @16:48:00.70 SS.seek 0ml @16:48:10.02 Exhaust.select :close @16:48:10.14 SSV.dial CBV @16:48:10.17 CBV.dial SSV @16:48:11.03 Allowing 130s for 25.0ml of remaining 500.0ml @16:48:11.04 Intake.select :open @16:48:13.80 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:48:13.82 SS.reconfigure SSinhale.with maxSpeed: 32 @16:48:13.87 SS.seek 25ml @16:49:08.74 Stroke completed -- waiting for pressure to equalize ... @16:49:13.27 Intake.select :close @16:49:13.43 SSV.dial :exhaust @16:49:14.27 Exhaust.select :open @16:49:14.38 SS.reconfigure SSexhale @16:49:14.44 SS.seek 0ml @16:49:21.46 AFV.select :carrierSolution @16:49:21.57 delay 2 seconds @16:49:23.61 Exhaust.select :close @16:49:23.67 AS.reconfigure ASconfig @16:49:23.72 AS.seek 1ml @16:49:23.78 SSV.dial CBV @16:49:23.81 CBV.dial SSV @16:49:24.64 Allowing 130s for 25.0ml of remaining 475.0ml @16:49:24.65 Intake.select :open @16:49:26.84 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:49:26.86 SS.reconfigure SSinhale.with maxSpeed: 32 @16:49:26.91 SS.seek 25ml @16:50:11.73 delay 1 second @16:50:12.74 AFV.select :holdingCoil @16:50:12.85 delay 1 second @16:50:13.87 ASV.dial :wasteD @16:50:14.83 AS.seek 0ml @16:50:21.90 Stroke completed -- waiting for pressure to equalize ... @16:50:27.07 Intake.select :close @16:50:27.24 SSV.dial :exhaust @16:50:28.05 Exhaust.select :open @16:50:28.16 SS.reconfigure SSexhale @16:50:28.22 SS.seek 0ml @16:50:37.40 Exhaust.select :close @16:50:37.52 SSV.dial CBV @16:50:37.55 CBV.dial SSV @16:50:38.39 Allowing 130s for 25.0ml of remaining 450.0ml @16:50:38.40 Intake.select :open @16:50:40.58 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:50:40.60 SS.reconfigure SSinhale.with maxSpeed: 32 @16:50:40.65 SS.seek 25ml @16:51:12.62 Priming Eluate @16:51:12.63 AFV.select :carrierSolution @16:51:12.74 delay 2 seconds @16:51:14.77 AS.seek 0.5ml @16:51:35.51 Stroke completed -- waiting for pressure to equalize ... @16:51:40.68 Intake.select :close @16:51:40.85 SSV.dial :exhaust @16:51:41.66 Exhaust.select :open @16:51:41.77 SS.reconfigure SSexhale @16:51:41.83 SS.seek 0ml @16:51:43.84 delay 1 second @16:51:44.86 AFV.select :holdingCoil @16:51:44.96 ATV.dial ASV @16:51:44.97 ASV.dial :SPErecovery @16:51:46.10 Swish #1 of 2 @16:51:46.18 AS.reconfigure ASslow0 @16:51:46.23 AS.seek 0.6ml @16:51:50.84 Exhaust.select :close @16:51:50.96 SSV.dial CBV @16:51:50.99 CBV.dial SSV @16:51:51.83 Allowing 130s for 25.0ml of remaining 425.0ml @16:51:51.84 Intake.select :open @16:51:54.02 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:51:54.04 SS.reconfigure SSinhale.with maxSpeed: 32 @16:51:54.09 SS.seek 25ml @16:51:56.70 AS.seek 0.5ml @16:52:07.08 Swish #2 of 2 @16:52:07.17 AS.seek 0.6ml @16:52:17.64 AS.seek 0.5ml @16:52:28.04 delay 3 seconds @16:52:31.13 AS.reconfigure ASslow1 @16:52:31.18 AS.seek 0.684ml @16:52:49.04 Stroke completed -- waiting for pressure to equalize ... @16:52:54.85 Intake.select :close @16:52:55.01 SSV.dial :exhaust @16:52:55.83 Exhaust.select :open @16:52:55.94 SS.reconfigure SSexhale @16:52:56.00 SS.seek 0ml @16:53:05.11 Exhaust.select :close @16:53:05.22 SSV.dial CBV @16:53:05.25 CBV.dial SSV @16:53:06.08 Allowing 130s for 25.0ml of remaining 400.0ml @16:53:06.09 Intake.select :open @16:53:08.28 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:53:08.30 SS.reconfigure SSinhale.with maxSpeed: 32 @16:53:08.35 SS.seek 25ml @16:53:08.48 delay 3 seconds @16:53:11.50 Eluate primed @16:53:11.51 ATV.dial :waste2 @16:53:12.09 AS.reconfigure ASnorm @16:53:12.14 AS.seek 0ml @16:53:46.94 SPE.coast @16:53:47.03 ASV.dial :SPErecovery @16:53:47.05 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0616h1500ml.pcr @16:53:47.07 Building PCR Slug from ASV with MM2 mix, PP4 primer @16:53:47.08 Priming with carrier @16:53:47.09 ATV.dial ARV @16:53:47.99 ARV.dial :carrier @16:53:48.34 AS.seek 0.5ml @16:54:03.33 Stroke completed -- waiting for pressure to equalize ... @16:54:09.10 Intake.select :close @16:54:09.27 SSV.dial :exhaust @16:54:10.08 Exhaust.select :open @16:54:10.19 SS.reconfigure SSexhale @16:54:10.24 SS.seek 0ml @16:54:13.90 AFV.select :carrierSolution @16:54:14.02 delay 2 seconds @16:54:16.05 AS.reconfigure ASconfig @16:54:16.10 AS.seek 1ml @16:54:19.07 Exhaust.select :close @16:54:19.20 SSV.dial CBV @16:54:19.91 CBV.dial SSV @16:54:20.69 Allowing 130s for 25.0ml of remaining 375.0ml @16:54:20.70 Intake.select :open @16:54:22.88 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:54:22.90 SS.reconfigure SSinhale.with maxSpeed: 32 @16:54:22.95 SS.seek 25ml @16:54:45.12 delay 1 second @16:54:46.14 AFV.select :holdingCoil @16:54:46.25 delay 1 second @16:54:47.27 ATV.dial :waste @16:54:48.67 AS.reconfigure ASnorm @16:54:48.72 AS.seek :empty @16:55:12.61 Vacuum too high -- waiting up to 40s @16:55:13.98 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:55:14.00 SS.reconfigure SSinhale.with maxSpeed: 29 @16:55:14.05 SS.seek 25ml @16:55:20.66 Stroke completed -- waiting for pressure to equalize ... @16:55:25.18 Intake.select :close @16:55:25.34 SSV.dial :exhaust @16:55:26.16 Exhaust.select :open @16:55:26.27 SS.reconfigure SSexhale @16:55:26.32 SS.seek 0ml @16:55:35.84 Exhaust.select :close @16:55:35.95 SSV.dial CBV @16:55:35.98 CBV.dial SSV @16:55:36.78 Allowing 130s for 25.0ml of remaining 350.0ml @16:55:36.79 Intake.select :open @16:55:38.98 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:55:39.00 SS.reconfigure SSinhale.with maxSpeed: 29 @16:55:39.05 SS.seek 25ml @16:55:39.45 AFV.select :carrierSolution @16:55:39.57 delay 2 seconds @16:55:41.60 AS.reconfigure ASconfig @16:55:41.65 AS.seek 0.5ml @16:56:10.76 delay 1 second @16:56:11.77 AFV.select :holdingCoil @16:56:11.88 Building Slug @16:56:11.89 ATV.dial :ARV @16:56:13.40 delay 2 seconds @16:56:15.42 ARV.dial :air1 @16:56:15.84 delay 2 seconds @16:56:17.93 AS.reconfigure ASslow2 @16:56:17.99 AS.seek 0.506ml @16:56:24.46 delay 2 seconds @16:56:26.48 ARV.dial :MM2 @16:56:27.87 delay 2 seconds @16:56:29.90 Adding MM2 @16:56:29.98 AS.seek 0.524ml @16:56:39.77 Stroke completed -- waiting for pressure to equalize ... @16:56:44.92 Intake.select :close @16:56:45.10 SSV.dial :exhaust @16:56:45.91 Exhaust.select :open @16:56:46.02 SS.reconfigure SSexhale @16:56:46.07 SS.seek 0ml @16:56:48.21 delay 25 seconds @16:56:55.27 Exhaust.select :close @16:56:55.39 SSV.dial CBV @16:56:55.42 CBV.dial SSV @16:56:56.84 Allowing 130s for 25.0ml of remaining 325.0ml @16:56:56.85 Intake.select :open @16:56:59.04 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:56:59.06 SS.reconfigure SSinhale.with maxSpeed: 29 @16:56:59.11 SS.seek 25ml @16:57:13.22 ARV.dial :PP4 @16:57:13.60 Adding PP4 @16:57:13.62 delay 2 seconds @16:57:15.70 AS.seek 0.53ml @16:57:22.26 delay 10 seconds @16:57:32.29 ARV.dial :air3 @16:57:32.67 adding ARV air @16:57:32.69 delay 2 seconds @16:57:34.77 AS.seek 0.536ml @16:57:40.98 delay 5 seconds @16:57:46.00 ARV.dial :carrier @16:57:47.18 delay 7 seconds @16:57:54.27 AS.seek 0.594ml @16:57:59.66 Stroke completed -- waiting for pressure to equalize ... @16:58:05.45 Intake.select :close @16:58:05.61 SSV.dial :exhaust @16:58:06.41 Exhaust.select :open @16:58:06.52 SS.reconfigure SSexhale @16:58:06.57 SS.seek 0ml @16:58:15.93 Exhaust.select :close @16:58:16.05 SSV.dial CBV @16:58:16.08 CBV.dial SSV @16:58:16.91 Allowing 130s for 25.0ml of remaining 300.0ml @16:58:16.92 Intake.select :open @16:58:19.11 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:58:19.13 SS.reconfigure SSinhale.with maxSpeed: 29 @16:58:19.18 SS.seek 25ml @16:58:52.74 delay 5 seconds @16:58:57.76 ATV.dial :ASV @16:58:58.15 delay 2 seconds @16:59:00.24 AS.seek 0.6ml @16:59:06.53 delay 4 seconds @16:59:10.55 ATV.dial ARV @16:59:11.18 delay 2 seconds @16:59:13.20 ARV.dial :carrier @16:59:13.22 delay 2 seconds @16:59:15.31 AS.seek 0.65ml @16:59:20.01 Stroke completed -- waiting for pressure to equalize ... @16:59:25.82 Intake.select :close @16:59:25.98 SSV.dial :exhaust @16:59:26.80 Exhaust.select :open @16:59:26.91 SS.reconfigure SSexhale @16:59:26.96 SS.seek 0ml @16:59:36.04 Exhaust.select :close @16:59:36.16 SSV.dial CBV @16:59:36.19 CBV.dial SSV @16:59:37.00 Allowing 130s for 25.0ml of remaining 275.0ml @16:59:37.01 Intake.select :open @16:59:39.20 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:59:39.22 SS.reconfigure SSinhale.with maxSpeed: 29 @16:59:39.27 SS.seek 25ml @17:00:05.87 delay 4 seconds @17:00:09.89 ATV.dial PCR @17:00:10.48 ASV.dial :waste1 @17:00:11.21 ARV.dial :air1 @17:00:11.59 Positioning Slug by 0.324ml @17:00:11.68 AS.seek 0.326ml @17:00:40.65 Stroke completed -- waiting for pressure to equalize ... @17:00:46.47 Intake.select :close @17:00:46.63 SSV.dial :exhaust @17:00:47.42 Exhaust.select :open @17:00:47.53 SS.reconfigure SSexhale @17:00:47.58 SS.seek 0ml @17:00:56.50 Exhaust.select :close @17:00:56.61 SSV.dial CBV @17:00:56.64 CBV.dial SSV @17:00:57.47 Allowing 130s for 25.0ml of remaining 250.0ml @17:00:57.48 Intake.select :open @17:00:59.66 Intake @34.7psia, vacuum from 20.0 to 24.0psi @17:00:59.68 SS.reconfigure SSinhale.with maxSpeed: 29 @17:00:59.73 SS.seek 25ml @17:02:00.60 Stroke completed -- waiting for pressure to equalize ... @17:02:07.04 Intake.select :close @17:02:07.21 SSV.dial :exhaust @17:02:08.00 Exhaust.select :open @17:02:08.11 SS.reconfigure SSexhale @17:02:08.17 SS.seek 0ml @17:02:17.05 Exhaust.select :close @17:02:17.17 SSV.dial CBV @17:02:17.20 CBV.dial SSV @17:02:18.02 Allowing 130s for 25.0ml of remaining 225.0ml @17:02:18.03 Intake.select :open @17:02:20.22 Intake @34.6psia, vacuum from 20.0 to 24.0psi @17:02:20.24 SS.reconfigure SSinhale.with maxSpeed: 29 @17:02:20.29 SS.seek 25ml @17:03:21.13 Stroke completed -- waiting for pressure to equalize ... @17:03:27.56 Intake.select :close @17:03:27.72 SSV.dial :exhaust @17:03:29.12 Exhaust.select :open @17:03:29.23 SS.reconfigure SSexhale @17:03:29.28 SS.seek 0ml @17:03:38.47 Exhaust.select :close @17:03:38.59 SSV.dial CBV @17:03:38.62 CBV.dial SSV @17:03:39.44 Allowing 130s for 25.0ml of remaining 200.0ml @17:03:39.45 Intake.select :open @17:03:41.64 Intake @34.6psia, vacuum from 20.0 to 24.0psi @17:03:41.66 SS.reconfigure SSinhale.with maxSpeed: 29 @17:03:41.71 SS.seek 25ml @17:04:30.60 Vacuum too high -- waiting up to 40s @17:04:32.28 Intake @34.4psia, vacuum from 20.0 to 24.0psi @17:04:32.31 SS.reconfigure SSinhale.with maxSpeed: 26 @17:04:32.36 SS.seek 25ml @17:04:45.74 Stroke completed -- waiting for pressure to equalize ... @17:04:50.90 Intake.select :close @17:04:51.06 SSV.dial :exhaust @17:04:51.87 Exhaust.select :open @17:04:51.98 SS.reconfigure SSexhale @17:04:52.04 SS.seek 0ml @17:05:01.11 Exhaust.select :close @17:05:01.23 SSV.dial CBV @17:05:01.26 CBV.dial SSV @17:05:02.06 Allowing 130s for 25.0ml of remaining 175.0ml @17:05:02.07 Intake.select :open @17:05:04.26 Intake @34.7psia, vacuum from 20.0 to 24.0psi @17:05:04.28 SS.reconfigure SSinhale.with maxSpeed: 26 @17:05:04.33 SS.seek 25ml @17:05:37.81 delay 5 seconds @17:05:42.83 ATV.dial ARV @17:05:43.67 ASV.dial :PCRlock @17:05:44.04 delay 5 seconds @17:05:49.07 Running Tt1 Profile60C300 thermocycling @17:05:49.08 PCR.program Profile60C300 @17:05:49.22 Profile60C300 should complete in < 1:45:21 @17:05:49.23 PCR.run 2:16:41.25 @17:06:11.25 Stroke completed -- waiting for pressure to equalize ... @17:06:17.03 Intake.select :close @17:06:17.20 SSV.dial :exhaust @17:06:18.01 Exhaust.select :open @17:06:18.12 SS.reconfigure SSexhale @17:06:18.18 SS.seek 0ml @17:06:27.52 Exhaust.select :close @17:06:27.64 SSV.dial CBV @17:06:27.68 CBV.dial SSV @17:06:28.53 Allowing 130s for 25.0ml of remaining 150.0ml @17:06:28.54 Intake.select :open @17:06:31.33 Intake @34.6psia, vacuum from 20.0 to 24.0psi @17:06:31.35 SS.reconfigure SSinhale.with maxSpeed: 26 @17:06:31.40 SS.seek 25ml @17:07:38.83 Stroke completed -- waiting for pressure to equalize ... @17:07:44.68 Intake.select :close @17:07:44.85 SSV.dial :exhaust @17:07:45.66 Exhaust.select :open @17:07:45.77 SS.reconfigure SSexhale @17:07:45.83 SS.seek 0ml @17:07:54.91 Exhaust.select :close @17:07:55.03 SSV.dial CBV @17:07:55.06 CBV.dial SSV @17:07:55.89 Allowing 130s for 25.0ml of remaining 125.0ml @17:07:55.90 Intake.select :open @17:07:58.09 Intake @34.7psia, vacuum from 20.0 to 24.0psi @17:07:58.11 SS.reconfigure SSinhale.with maxSpeed: 26 @17:07:58.16 SS.seek 25ml @17:09:05.58 Stroke completed -- waiting for pressure to equalize ... @17:09:12.02 Intake.select :close @17:09:12.19 SSV.dial :exhaust @17:09:12.98 Exhaust.select :open @17:09:13.09 SS.reconfigure SSexhale @17:09:13.14 SS.seek 0ml @17:09:22.18 Exhaust.select :close @17:09:22.30 SSV.dial CBV @17:09:22.33 CBV.dial SSV @17:09:23.16 Allowing 130s for 25.0ml of remaining 100.0ml @17:09:23.17 Intake.select :open @17:09:25.35 Intake @34.7psia, vacuum from 20.0 to 24.0psi @17:09:25.37 SS.reconfigure SSinhale.with maxSpeed: 26 @17:09:25.42 SS.seek 25ml @17:10:32.72 Stroke completed -- waiting for pressure to equalize ... @17:10:39.14 Intake.select :close @17:10:39.30 SSV.dial :exhaust @17:10:40.11 Exhaust.select :open @17:10:40.23 SS.reconfigure SSexhale @17:10:40.29 SS.seek 0ml @17:10:49.50 Exhaust.select :close @17:10:49.62 SSV.dial CBV @17:10:49.65 CBV.dial SSV @17:10:50.45 Allowing 130s for 25.0ml of remaining 75.0ml @17:10:50.46 Intake.select :open @17:10:52.65 Intake @34.7psia, vacuum from 20.0 to 24.0psi @17:10:52.67 SS.reconfigure SSinhale.with maxSpeed: 26 @17:10:52.72 SS.seek 25ml @17:12:00.18 Stroke completed -- waiting for pressure to equalize ... @17:12:07.25 Intake.select :close @17:12:07.42 SSV.dial :exhaust @17:12:08.23 Exhaust.select :open @17:12:08.35 SS.reconfigure SSexhale @17:12:08.40 SS.seek 0ml @17:12:17.47 Exhaust.select :close @17:12:17.59 SSV.dial CBV @17:12:17.62 CBV.dial SSV @17:12:18.44 Allowing 130s for 25.0ml of remaining 50.0ml @17:12:18.45 Intake.select :open @17:12:20.63 Intake @34.7psia, vacuum from 20.0 to 24.0psi @17:12:20.65 SS.reconfigure SSinhale.with maxSpeed: 26 @17:12:20.70 SS.seek 25ml @17:13:28.18 Stroke completed -- waiting for pressure to equalize ... @17:13:35.25 Intake.select :close @17:13:35.41 SSV.dial :exhaust @17:13:36.84 Exhaust.select :open @17:13:36.95 SS.reconfigure SSexhale @17:13:37.00 SS.seek 0ml @17:13:46.19 Exhaust.select :close @17:13:46.30 SSV.dial CBV @17:13:46.33 CBV.dial SSV @17:13:47.14 Allowing 130s for 25.0ml of remaining 25.0ml @17:13:47.15 Intake.select :open @17:13:49.33 Intake @34.8psia, vacuum from 20.0 to 24.0psi @17:13:49.35 SS.reconfigure SSinhale.with maxSpeed: 26 @17:13:49.40 SS.seek 24.96ml @17:14:38.51 Vacuum too high -- waiting up to 40s @17:14:40.15 Intake @34.4psia, vacuum from 20.0 to 24.0psi @17:14:40.18 SS.reconfigure SSinhale.with maxSpeed: 23 @17:14:40.23 SS.seek 24.96ml @17:15:00.50 Stroke completed -- waiting for pressure to equalize ... @17:15:06.32 Intake.select :close @17:15:06.48 SSV.dial :exhaust @17:15:07.30 Exhaust.select :open @17:15:07.41 SS.reconfigure SSexhale @17:15:07.46 SS.seek 0ml @17:15:16.39 Exhaust.select :close @17:15:16.51 Sampled 1500.0ml @17:15:16.53 Drying puck before fixing for 4:00 @17:15:16.54 CBV.dial :waste2b @17:15:17.33 delay 1:00 @17:16:17.35 CSV.dial :air @17:16:17.81 CS.reconfigure CSfast @17:16:17.87 CS.seek 10ml @17:16:41.80 Pumping wcr puck from tube 2 up to 55psi... @17:16:41.81 CBV.dial SSV @17:16:43.18 CBV.dial between CSV and waste2b @17:16:44.18 CSV.dial between air and CTV @17:16:44.54 CS.seek 6.936ml @17:16:52.52 CTV.dial CSV,avoiding: :air @17:16:52.55 CSV.dial CTV @17:16:53.38 CS.reconfigure CSpressurize @17:16:53.44 CS.seek 0ml @17:17:11.83 CSV.dial :air @17:17:12.31 CS.reconfigure CSfast @17:17:12.37 CS.seek 10ml @17:17:36.21 CSV.dial between air and CTV @17:17:36.56 CS.seek 2.247ml @17:17:55.14 CTV.dial CSV,avoiding: :air @17:17:55.17 CSV.dial CTV @17:17:55.49 CS.reconfigure CSpressurize @17:17:55.55 CS.seek 0ml @17:17:58.38 CBV.dial :waste2b @17:17:58.74 CS.reconfigure CSconfig @17:17:58.79 CS.seek :empty @17:18:03.11 delay 1:00 @17:19:03.14 CTV.dial :air,avoiding: :intake @17:19:04.49 Fix #1 of 2 (with rnal) for 2:00 @17:19:04.51 CSV.dial CSR @17:19:05.05 Pulling 2ml of CSR.rnal @17:19:05.08 CSR.select :rnal @17:19:05.24 CS.seek 2ml @17:19:11.33 delay 5 seconds @17:19:16.35 CSR.select :air @17:19:16.48 CS.reconfigure CSfast @17:19:16.53 CS.seek 10ml @17:19:35.55 Pumping wcr puck from tube 2 up to 55psi... @17:19:35.56 CBV.dial SSV @17:19:36.91 CBV.dial between CSV and waste2b @17:19:37.92 CSV.dial between air and CTV @17:19:38.56 CS.seek 6.829ml @17:19:46.80 CTV.dial CSV,avoiding: :air @17:19:46.83 CSV.dial CTV @17:19:48.24 CS.reconfigure CSpressurize @17:19:48.30 CS.seek 0ml @17:20:06.42 CSV.dial :air @17:20:06.91 CS.reconfigure CSfast @17:20:06.97 CS.seek 10ml @17:20:30.67 CSV.dial between air and CTV @17:20:31.03 CS.seek 2.017ml @17:20:50.30 CTV.dial CSV,avoiding: :air @17:20:50.33 CSV.dial CTV @17:20:50.66 CS.reconfigure CSpressurize @17:20:50.71 CS.seek 0ml @17:20:52.61 delay 2:00 @17:22:52.64 CBV.dial :waste2b @17:22:52.99 CS.reconfigure CSconfig @17:22:53.04 CS.seek :empty @17:22:58.37 delay 1:00 @17:23:58.40 CTV.dial :air,avoiding: :intake @17:23:59.74 Fix #2 of 2 (with rnal) for 2:00 @17:23:59.76 CSV.dial CSR @17:24:00.29 Pulling 2ml of CSR.rnal @17:24:00.31 CSR.select :rnal @17:24:00.43 CS.seek 2ml @17:24:06.62 delay 5 seconds @17:24:11.64 CSR.select :air @17:24:11.78 CS.reconfigure CSfast @17:24:11.83 CS.seek 10ml @17:24:30.93 Pumping wcr puck from tube 2 up to 55psi... @17:24:30.95 CBV.dial SSV @17:24:32.31 CBV.dial between CSV and waste2b @17:24:33.34 CSV.dial between air and CTV @17:24:33.99 CS.seek 6.829ml @17:24:42.18 CTV.dial CSV,avoiding: :air @17:24:42.22 CSV.dial CTV @17:24:43.63 CS.reconfigure CSpressurize @17:24:43.71 CS.seek 0ml @17:25:01.77 CSV.dial :air @17:25:02.26 CS.reconfigure CSfast @17:25:02.32 CS.seek 10ml @17:25:26.00 CSV.dial between air and CTV @17:25:26.35 CS.seek 1.994ml @17:25:45.53 CTV.dial CSV,avoiding: :air @17:25:45.56 CSV.dial CTV @17:25:45.87 CS.reconfigure CSpressurize @17:25:45.93 CS.seek 0ml @17:25:47.08 delay 2:00 @17:27:47.10 CBV.dial :waste2b @17:27:48.05 CS.reconfigure CSconfig @17:27:48.12 CS.seek :empty @17:27:53.81 delay 1:00 @17:28:53.84 CTV.dial :air,avoiding: :intake @17:28:55.27 Storing wcr puck in Collection Clamp to tube 1 @17:28:55.47 SC.reconfigure SCtight @17:28:55.52 SC.seek 1 @17:28:57.67 SC.reconfigure SCloose @17:28:57.91 Elbow.reconfigure TightElbow @17:28:57.97 Elbow.seek 1 @17:28:59.91 Forearm.reconfigure TightForearm @17:28:59.97 Forearm.seek 1 + 700 counts @17:29:01.12 SE.reconfigure SEpick @17:29:01.19 SE.seek beamBreak @17:29:02.53 SE.configure SEconfig @17:29:02.71 Elbow.reconfigure LimpElbow @17:29:02.76 Forearm.reconfigure LooseForearm @17:29:02.81 Forearm.seek 1 @17:29:03.14 Elbow.hold @17:29:03.22 Elbow.reconfigure TightElbow @17:29:03.33 SE.seek 19.1 Pucks below Top Plate @17:29:03.80 Forearm.force -20 @17:29:03.85 Forearm.seek 1 + 346 counts @17:29:04.17 Forearm.reconfigure TightForearm @17:29:04.23 Forearm.seek 1 + 700 counts @17:29:04.54 SE.seek 20.1 Pucks below Top Plate @17:29:05.20 Forearm.reconfigure LooseForearm @17:29:05.25 Forearm.seek :retract @17:29:06.17 Elbow.seek CC @17:29:07.88 Elbow.reconfigure LooseElbow @17:29:07.99 Forearm.seek CC @17:29:08.96 Hand.close @17:29:09.63 CC.seek :open @17:29:14.13 Forearm.seek :retract @17:29:15.14 Elbow.reconfigure TightElbow @17:29:15.19 Elbow.seek 1 @17:29:17.10 Elbow.reconfigure LimpElbow @17:29:17.16 Forearm.seek 1 @17:29:18.17 Elbow.hold @17:29:18.85 Elbow.reconfigure TightElbow @17:29:18.91 Hand.open @17:29:19.64 Forearm.force -20 @17:29:19.69 Forearm.seek 1 + 347 counts @17:29:20.02 Forearm.reconfigure TightForearm @17:29:20.08 Forearm.seek 1 + 700 counts @17:29:20.47 SE.seek :down @17:29:21.32 SE.coast @17:29:21.63 Forearm.reconfigure LooseForearm @17:29:21.69 Forearm.seek :retract @17:29:22.63 SC.coast @17:29:22.67 Starting finalPurge @17:29:22.69 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:29:22.77 Hand.open @17:29:23.15 Elbow.seek FlushPuck::Garage @17:29:25.18 Elbow.reconfigure LooseElbow @17:29:25.29 Forearm.seek :garage @17:29:26.28 Hand.close @17:29:27.02 Forearm.seek :retract @17:29:28.03 Elbow.reconfigure TightElbow @17:29:28.08 Elbow.seek CC @17:29:31.04 Elbow.reconfigure LooseElbow @17:29:31.15 Forearm.reconfigure TightForearm @17:29:31.20 Forearm.seek CC @17:29:32.50 CC.seek :closed @17:29:36.77 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.95 @17:29:36.86 Hand.open @17:29:37.57 Forearm.reconfigure LooseForearm @17:29:37.62 Forearm.seek :retract @17:29:38.58 Flushing the Collection Valve::Manifold @17:29:38.75 CSV.dial CSR @17:29:39.28 Pulling 5ml of CSR.flush @17:29:39.29 CSR.select :flush @17:29:39.42 CS.seek 5ml @17:29:53.15 delay 5 seconds @17:29:58.17 CSR.select :air @17:29:58.36 CS.reconfigure CSfast @17:29:58.41 CS.seek 7.5ml @17:30:05.05 CSV.dial :waste @17:30:06.37 CS.reconfigure CSconfig @17:30:06.42 CS.seek 0ml @17:30:26.56 Flushing lower manifold's waste1 port @17:30:26.70 CSV.dial :flush @17:30:27.18 CS.reconfigure CSfast @17:30:27.23 CS.seek 5ml @17:30:39.47 delay 5 seconds @17:30:44.49 CSV.dial :air @17:30:45.03 CS.seek 7ml @17:30:50.59 CSV.dial CTV @17:30:50.62
CTV.dial CSV @17:30:52.05 CBV.dial :waste1 @17:30:52.85 CS.seek 0ml @17:31:09.59 Flushing lower manifold's waste2b port @17:31:09.73 CSV.dial :flush @17:31:10.51 CS.seek 5ml @17:31:22.94 delay 5 seconds @17:31:27.96 CSV.dial :air @17:31:28.51 CS.seek 7ml @17:31:34.06 CSV.dial CTV @17:31:34.09
CTV.dial CSV @17:31:34.56 CBV.dial :waste2b @17:31:35.37 CS.seek 0ml @17:31:52.16 Flushing upper manifold @17:31:52.30 CSV.dial :flush @17:31:53.07 CS.seek 5ml @17:32:05.34 delay 5 seconds @17:32:10.35 CSV.dial :air @17:32:10.91 CS.seek 7ml @17:32:16.50 CSV.dial CBV @17:32:16.55
CBV.dial CSV @17:32:17.61 CTV.dial :air @17:32:18.98 CS.seek 0ml @17:32:35.59 delay 7 seconds @17:32:42.61 CS.seek 3ml @17:32:50.47 CSV.dial :waste @17:32:50.96 CS.seek 0ml @17:32:58.87 CSV.dial :flush @17:32:59.35 CS.seek 3ml @17:33:07.34 delay 5 seconds @17:33:12.35 CSV.dial :air @17:33:12.90 CS.seek 5.001ml @17:33:18.46 CSV.dial :air @17:33:18.49 CS.seek 0ml @17:33:30.78 delay 7 seconds @17:33:37.80 CS.seek 3ml @17:33:45.55 CSV.dial :waste @17:33:46.32 CS.seek 0ml @17:33:54.09 Disinfecting external Intake with mfbkill @17:33:54.23 CSV.dial CSR @17:33:55.61 Pulling 8ml of CSR.mfbkill @17:33:55.62 CSR.select :mfbkill @17:33:55.75 CS.seek 8ml @17:34:14.72 delay 5 seconds @17:34:19.74 CSR.select :air @17:34:20.49 CS.seek 10ml @17:34:26.03 CSV.dial CBV @17:34:26.05
CBV.dial CSV @17:34:27.11 CTV.dial :intake @17:34:27.89 Intake.select :open @17:34:28.01 CS.seek 2ml @17:34:47.62 Intake.select :close @17:34:47.74 CTV.dial :air @17:34:48.53 CS.reconfigure CSconfig @17:34:48.58 CS.seek 0ml @17:34:54.63 Flushing lower manifold's waste1 port @17:34:54.64 CSV.dial CSR @17:34:55.77 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @17:34:55.90 CS.seek 4ml @17:35:07.10 delay 5 seconds @17:35:12.12 CSR.select :air @17:35:12.25 CS.reconfigure CSfast @17:35:12.30 CS.seek 10ml @17:35:26.88 CSV.dial CTV @17:35:26.91
CTV.dial CSV @17:35:28.36 CBV.dial :waste1 @17:35:28.86 CS.seek 0ml @17:35:52.41 Flushing lower manifold's waste2b port @17:35:52.42 CSV.dial CSR @17:35:52.96 Pulling 4ml of CSR.mfbkill @17:35:52.97 CSR.select :mfbkill @17:35:53.09 CS.reconfigure CSconfig @17:35:53.13 CS.seek 4ml @17:36:04.69 delay 5 seconds @17:36:09.71 CSR.select :air @17:36:09.83 CS.reconfigure CSfast @17:36:09.88 CS.seek 10ml @17:36:24.41 CSV.dial CTV @17:36:24.44
CTV.dial CSV @17:36:24.92 CBV.dial :waste2b @17:36:25.72 CS.seek 0ml @17:36:49.16 Killing upper manifold @17:36:49.17 CSV.dial CSR @17:36:49.70 Pulling 4ml of CSR.mfbkill @17:36:49.71 CSR.select :mfbkill @17:36:49.84 CS.reconfigure CSconfig @17:36:49.88 CS.seek 4ml @17:37:01.13 delay 5 seconds @17:37:06.14 CSR.select :air @17:37:06.27 CS.reconfigure CSfast @17:37:06.32 CS.seek 10ml @17:37:20.85 CSV.dial CBV @17:37:20.88
CBV.dial CSV @17:37:21.97 CTV.dial :air @17:37:23.33 CS.seek 0ml @17:37:46.66 delay 7 seconds @17:37:53.69 CS.seek 3ml @17:38:01.90 CSV.dial :waste @17:38:02.37 CS.seek 0ml @17:38:10.10 Flushing upper manifold @17:38:10.24 CSV.dial :flush @17:38:10.72 CS.seek 5ml @17:38:22.97 delay 5 seconds @17:38:27.98 CSV.dial :air @17:38:28.53 CS.seek 7.001ml @17:38:34.76 CSV.dial CBV @17:38:34.79
CBV.dial CSV @17:38:35.86 CTV.dial :air @17:38:35.88 CS.seek 0ml @17:38:52.72 delay 7 seconds @17:38:59.75 CS.seek 3ml @17:39:07.78 CSV.dial :waste @17:39:08.28 CS.seek 0ml @17:39:16.14 CSV.dial :flush @17:39:16.61 CS.seek 3ml @17:39:24.41 delay 5 seconds @17:39:29.43 CSV.dial :air @17:39:29.98 CS.seek 5ml @17:39:35.47 CSV.dial :air @17:39:35.49 CS.seek 0ml @17:39:47.82 delay 7 seconds @17:39:54.85 CS.seek 3ml @17:40:02.57 CSV.dial :waste @17:40:03.34 CS.seek 0ml @17:40:11.07 Flushing lower manifold's waste1 port @17:40:11.21 CSV.dial :flush @17:40:11.69 CS.seek 5ml @17:40:23.85 delay 5 seconds @17:40:28.86 CSV.dial :air @17:40:29.40 CS.seek 7ml @17:40:34.94 CSV.dial CTV @17:40:34.97
CTV.dial CSV @17:40:36.40 CBV.dial :waste1 @17:40:36.89 CS.seek 0ml @17:40:53.63 Flushing lower manifold's waste2b port @17:40:53.77 CSV.dial :flush @17:40:54.55 CS.seek 5ml @17:41:06.91 delay 5 seconds @17:41:11.93 CSV.dial :air @17:41:12.50 CS.seek 7ml @17:41:18.04 CSV.dial CTV @17:41:18.08
CTV.dial CSV @17:41:18.54 CBV.dial :waste2b @17:41:19.35 CS.seek 0ml @17:41:36.04 Disinfecting external Intake with kill @17:41:36.18 CSV.dial CSR @17:41:36.72 Pulling 8ml of CSR.kill CSR.select :kill @17:41:36.85 CS.seek 8ml @17:41:55.85 delay 5 seconds @17:42:00.87 CSR.select :air @17:42:01.01 CS.seek 10ml @17:42:06.73 CSV.dial CBV @17:42:06.76
CBV.dial CSV @17:42:07.87 CTV.dial :intake @17:42:08.63 Intake.select :open @17:42:08.75 CS.seek 2ml @17:42:28.35 Intake.select :close @17:42:28.46 CTV.dial :air @17:42:29.84 CS.reconfigure CSconfig @17:42:29.89 CS.seek 0ml @17:42:35.97 CSV.dial CSR @17:42:37.11 Pulling 5ml of CSR.flush CSR.select :flush @17:42:37.24 CS.seek 5ml @17:42:51.01 delay 5 seconds @17:42:56.03 CSR.select :air @17:42:56.17 CS.seek 10ml @17:43:09.91 CSV.dial CBV @17:43:10.97 CS.seek 0ml @17:43:37.48 Garaging FlushPuck in Collection Clamp @17:43:37.56 Elbow.reconfigure TightElbow @17:43:37.61 Elbow.seek CC @17:43:37.87 Elbow.reconfigure LooseElbow @17:43:37.98 Forearm.seek CC @17:43:38.93 Hand.close @17:43:39.52 CC.seek :open @17:43:43.89 Forearm.seek :retract @17:43:44.87 Elbow.reconfigure TightElbow @17:43:44.92 Elbow.seek :garage @17:43:48.16 Elbow.reconfigure LooseElbow @17:43:48.27 Forearm.reconfigure TightForearm @17:43:48.32 Forearm.seek :garage @17:43:49.46 Hand.open @17:43:50.22 Forearm.reconfigure LooseForearm @17:43:50.27 Forearm.seek :retract @17:43:51.30 Starting initialPurge @17:43:51.31 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:43:51.39 Hand.open @17:43:51.72 Elbow.reconfigure TightElbow @17:43:51.77 Elbow.seek FlushPuck::Garage @17:43:51.89 Elbow.reconfigure LooseElbow @17:43:52.00 Forearm.seek :garage @17:43:53.02 Hand.close @17:43:53.78 Forearm.seek :retract @17:43:54.83 Elbow.reconfigure TightElbow @17:43:54.88 Elbow.seek CC @17:43:57.80 Elbow.reconfigure LooseElbow @17:43:57.91 Forearm.reconfigure TightForearm @17:43:57.96 Forearm.seek CC @17:43:59.25 CC.seek :closed @17:44:03.49 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.96 @17:44:03.59 Hand.open @17:44:04.34 Forearm.reconfigure LooseForearm @17:44:04.39 Forearm.seek :retract @17:44:05.37 Flushing the Collection Valve::Manifold @17:44:05.51 CSV.dial CSR @17:44:07.23 Pulling 5ml of CSR.flush @17:44:07.24 CSR.select :flush @17:44:07.37 CS.seek 5ml @17:44:21.14 delay 5 seconds @17:44:26.16 CSR.select :air @17:44:26.35 CS.reconfigure CSfast @17:44:26.40 CS.seek 7.5ml @17:44:33.09 CSV.dial :waste @17:44:34.43 CS.reconfigure CSconfig @17:44:34.48 CS.seek 0ml @17:44:54.64 Purging external Intake @17:44:54.78 CSV.dial :flush @17:44:55.26 CS.reconfigure CSfast @17:44:55.31 CS.seek 10ml @17:45:18.79 delay 5 seconds @17:45:23.81 CSV.dial CBV @17:45:23.84
CBV.dial CSV @17:45:24.64 CTV.dial :intake @17:45:25.43 Intake.select :open @17:45:25.55 CS.seek 0ml @17:45:49.75 Intake.select :close @17:45:49.87 CTV.dial :air @17:45:50.67 Flushing upper manifold @17:45:50.81 CSV.dial :flush @17:45:51.58 CS.seek 5ml @17:46:03.73 delay 5 seconds @17:46:08.74 CSV.dial :air @17:46:09.28 CS.seek 7ml @17:46:14.86 CSV.dial CBV @17:46:14.88
CBV.dial CSV @17:46:15.95 CTV.dial :air @17:46:15.97 CS.seek 0ml @17:46:32.70 delay 7 seconds @17:46:39.73 CS.seek 3ml @17:46:47.48 CSV.dial :waste @17:46:47.96 CS.seek 0ml @17:46:55.82 CSV.dial :flush @17:46:56.30 CS.seek 3ml @17:47:04.36 delay 5 seconds @17:47:09.37 CSV.dial :air @17:47:09.93 CS.seek 5ml @17:47:15.50 CSV.dial :air @17:47:15.53 CS.seek 0ml @17:47:27.92 delay 7 seconds @17:47:34.95 CS.seek 3ml @17:47:42.78 CSV.dial :waste @17:47:43.55 CS.seek 0ml @17:47:51.36 Garaging FlushPuck in Collection Clamp @17:47:51.44 Elbow.reconfigure TightElbow @17:47:51.49 Elbow.seek CC @17:47:51.74 Elbow.reconfigure LooseElbow @17:47:51.85 Forearm.seek CC @17:47:52.86 Hand.close @17:47:53.53 CC.seek :open @17:47:57.89 Forearm.seek :retract @17:47:58.90 Elbow.reconfigure TightElbow @17:47:58.95 Elbow.seek :garage @17:48:02.03 Elbow.reconfigure LooseElbow @17:48:02.14 Forearm.reconfigure TightForearm @17:48:02.80 Forearm.seek :garage @17:48:03.99 Hand.open @17:48:04.72 Forearm.reconfigure LooseForearm @17:48:04.77 Forearm.seek :retract @17:48:05.78 Completed [phasewithdil] begun 14:00:26 @17:48:05.80 Preparing to sleep until 14PM @17:48:05.85 deferred until MFBPCR finish @17:48:05.87
Waiting for MFBPCR til 14PM @17:48:05.90 Continuing at 14:00CEST07-Oct-18 @17:48:05.97 GoodNews.email "Then, in tube 2: phasewithdil\n/home/esp/esp2/mission/18tundeployv2dil.rb:118\n{1 => 21, 2 => 1}\nCan@17:48:05, 15.0C, 68% humidity, 14.4psia, 13.466V, 1.136A, 1.136A avg, 15.30W",Subject: "Waiting until 14:00CEST07-Oct-18" @18:28:10.18 PCR.off @18:28:11.74 PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0616h1500ml.pcr @18:28:11.82 Cleaning PCR @18:28:11.83 AFV.select :carrierSolution @18:28:11.94 delay 2 seconds @18:28:13.97 AS.reconfigure ASconfig @18:28:14.02 AS.seek 0.5ml @18:28:24.31 delay 1 second @18:28:25.32 AFV.select :holdingCoil @18:28:25.43 ASV.dial :waste1 @18:28:25.81 ATV.dial :air3 @18:28:26.99 AS.reconfigure ASslow1 @18:28:27.04 AS.seek 0.51ml @18:28:29.32 delay 2 seconds @18:28:31.34 ATV.dial :bleach @18:28:31.95 AS.seek 0.81ml @18:29:32.53 delay 3 seconds @18:29:35.55 ATV.dial :air3 @18:29:36.08 delay 3 seconds @18:29:39.17 AS.seek 0.835ml @18:29:44.45 delay 3 seconds @18:29:47.47 ATV.dial :bleach @18:29:48.07 AS.seek 0.82ml @18:29:51.38 delay 3 seconds @18:29:54.41 ARV.dial :waste @18:29:54.97 delay 3 seconds @18:29:57.99 ATV.dial :ARV @18:29:58.84 AS.reconfigure ASconfig @18:29:58.89 AS.seek 0.47ml @18:30:19.26 AFV.select :carrierSolution @18:30:19.38 delay 2 seconds @18:30:21.41 AS.seek 1ml @18:30:52.16 delay 1 second @18:30:53.17 AFV.select :holdingCoil @18:30:53.28 delay 1 second @18:30:54.31 AS.reconfigure ASfast @18:30:54.36 AS.seek 0ml @18:31:21.68 AFV.select :carrierSolution @18:31:21.80 delay 2 seconds @18:31:23.83 AS.reconfigure ASconfig @18:31:23.88 AS.seek 1ml @18:32:21.69 delay 1 second @18:32:22.70 AFV.select :holdingCoil @18:32:22.81 delay 1 second @18:32:23.84 AS.reconfigure ASfast @18:32:23.89 AS.seek 0ml @18:32:51.24 AFV.select :carrierSolution @18:32:51.36 delay 2 seconds @18:32:53.39 AS.reconfigure ASconfig @18:32:53.44 AS.seek 1ml @18:33:51.24 delay 1 second @18:33:52.25 AFV.select :holdingCoil @18:33:52.36 delay 1 second @18:33:53.39 AS.reconfigure ASfast @18:33:53.44 AS.seek 0ml @18:34:20.79 ARV.dial between carrier and air1 @18:34:21.38 ATV.dial :air3 @18:34:22.59 AS.reconfigure ASslow1 @18:34:22.64 AS.seek 0.01ml @18:34:24.99 delay 2 seconds @18:34:27.00 ATV.dial :bleach @18:34:28.19 AS.seek 0.81ml @18:37:09.38 delay 3 seconds @18:37:12.40 ATV.dial :air3 @18:37:12.93 delay 3 seconds @18:37:16.02 AS.seek 0.835ml @18:37:21.33 delay 3 seconds @18:37:24.35 ATV.dial :bleach @18:37:24.95 AS.seek 0.82ml @18:37:28.24 delay 3 seconds @18:37:31.26 ATV.dial PCR @18:37:31.65 AS.reconfigure ASconfig @18:37:31.70 AS.seek :empty @18:38:19.18 PCR.program :bleachProfile @18:38:19.24 PCR program should complete in < 2:45 @18:38:19.25 PCR.run 10:00 @18:41:27.13 PCR.off @18:41:27.15 AFV.select :carrierSolution @18:41:27.27 delay 2 seconds @18:41:29.30 AS.seek 1ml @18:42:27.13 delay 1 second @18:42:28.14 AFV.select :holdingCoil @18:42:28.25 delay 1 second @18:42:29.28 AS.reconfigure ASfast @18:42:29.33 AS.seek 0ml @18:42:56.66 delay 3 seconds @18:42:59.68 AFV.select :carrierSolution @18:42:59.79 delay 2 seconds @18:43:01.82 AS.reconfigure ASconfig @18:43:01.88 AS.seek 1ml @18:43:59.66 delay 1 second @18:44:00.67 AFV.select :holdingCoil @18:44:00.78 delay 1 second @18:44:01.81 AS.reconfigure ASfast @18:44:01.86 AS.seek 0ml @18:44:29.19 delay 3 seconds @18:44:32.21 AFV.select :carrierSolution @18:44:32.32 delay 2 seconds @18:44:34.35 AS.reconfigure ASconfig @18:44:34.40 AS.seek 1ml @18:45:32.18 delay 1 second @18:45:33.19 AFV.select :holdingCoil @18:45:33.30 delay 1 second @18:45:34.33 AS.reconfigure ASfast @18:45:34.38 AS.seek 0ml @18:46:01.73 delay 3 seconds @18:46:04.75 AFV.select :carrierSolution @18:46:04.86 delay 2 seconds @18:46:06.89 AS.reconfigure ASconfig @18:46:06.95 AS.seek 1ml @18:47:04.70 delay 1 second @18:47:05.71 AFV.select :holdingCoil @18:47:05.82 delay 1 second @18:47:06.85 AS.reconfigure ASfast @18:47:06.90 AS.seek 0ml @18:47:34.24 delay 3 seconds @18:47:37.26 AFV.select :carrierSolution @18:47:37.37 delay 2 seconds @18:47:39.40 AS.reconfigure ASconfig @18:47:39.46 AS.seek 1ml @18:48:37.26 delay 1 second @18:48:38.27 AFV.select :holdingCoil @18:48:38.38 delay 1 second @18:48:39.41 AS.reconfigure ASnorm @18:48:39.46 AS.seek 0ml @18:49:30.13 Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0618h1500ml.pcr @18:49:30.15 Building PCR Slug from ASV with MM2 mix, PP3 primer @18:49:30.17 Priming with carrier @18:49:30.18 ATV.dial ARV @18:49:30.76 ARV.dial :carrier @18:49:31.11 AS.seek 0.5ml @18:49:56.70 AFV.select :carrierSolution @18:49:56.82 delay 2 seconds @18:49:58.85 AS.reconfigure ASconfig @18:49:58.90 AS.seek 1ml @18:50:27.91 delay 1 second @18:50:28.92 AFV.select :holdingCoil @18:50:29.03 delay 1 second @18:50:30.05 ATV.dial :waste @18:50:31.59 AS.reconfigure ASnorm @18:50:31.64 AS.seek :empty @18:51:22.36 AFV.select :carrierSolution @18:51:22.48 delay 2 seconds @18:51:24.51 AS.reconfigure ASconfig @18:51:24.56 AS.seek 0.5ml @18:51:53.67 delay 1 second @18:51:54.68 AFV.select :holdingCoil @18:51:54.80 Building Slug @18:51:54.81 ATV.dial :ARV @18:51:56.42 delay 2 seconds @18:51:58.44 ARV.dial :air1 @18:51:58.85 delay 2 seconds @18:52:00.93 AS.reconfigure ASslow2 @18:52:00.99 AS.seek 0.506ml @18:52:07.29 delay 2 seconds @18:52:09.30 ARV.dial :MM2 @18:52:10.68 delay 2 seconds @18:52:12.71 Adding MM2 @18:52:13.39 AS.seek 0.524ml @18:52:31.65 delay 25 seconds @18:52:56.67 ARV.dial :PP3 @18:52:57.04 Adding PP3 @18:52:57.05 delay 2 seconds @18:52:59.14 AS.seek 0.53ml @18:53:05.40 delay 10 seconds @18:53:15.42 ARV.dial :air2 @18:53:15.81 adding ARV air @18:53:15.83 delay 2 seconds @18:53:17.91 AS.seek 0.536ml @18:53:24.18 delay 5 seconds @18:53:29.20 ARV.dial :carrier @18:53:30.40 delay 7 seconds @18:53:37.49 AS.seek 0.594ml @18:54:36.23 delay 5 seconds @18:54:41.25 ATV.dial :ASV @18:54:41.65 delay 2 seconds @18:54:43.74 AS.seek 0.6ml @18:54:49.88 delay 4 seconds @18:54:53.90 ATV.dial ARV @18:54:54.53 delay 2 seconds @18:54:56.55 ARV.dial :carrier @18:54:56.57 delay 2 seconds @18:54:58.66 AS.seek 0.65ml @18:55:49.23 delay 4 seconds @18:55:53.25 ATV.dial PCR @18:55:53.84 ASV.dial :waste1 @18:55:53.85 ARV.dial :air1 @18:55:54.35 Positioning Slug by 0.324ml @18:55:54.44 AS.seek 0.326ml @19:01:20.26 delay 5 seconds @19:01:25.28 ATV.dial ARV @19:01:26.01 ASV.dial :PCRlock @19:01:26.40 delay 5 seconds @19:01:31.43 Running Rp Profile60C300 thermocycling @19:01:31.44 PCR.program Profile60C300 @19:01:31.58 Profile60C300 should complete in < 1:45:21 @19:01:31.59 PCR.run 2:16:41.25 @20:23:29.51 PCR.off @20:23:31.08 PCR for Rp completed -- closed /var/log/esp/Rp18oct0618h1500ml.pcr @20:23:31.15 Cleaning PCR @20:23:31.16 AFV.select :carrierSolution @20:23:31.28 delay 2 seconds @20:23:33.31 AS.reconfigure ASconfig @20:23:33.36 AS.seek 0.5ml @20:23:43.68 delay 1 second @20:23:44.69 AFV.select :holdingCoil @20:23:44.80 ASV.dial :waste1 @20:23:45.18 ATV.dial :air3 @20:23:46.37 AS.reconfigure ASslow1 @20:23:46.42 AS.seek 0.51ml @20:23:48.74 delay 2 seconds @20:23:50.76 ATV.dial :bleach @20:23:51.36 AS.seek 0.81ml @20:24:52.02 delay 3 seconds @20:24:55.04 ATV.dial :air3 @20:24:55.57 delay 3 seconds @20:24:58.65 AS.seek 0.835ml @20:25:03.99 delay 3 seconds @20:25:07.01 ATV.dial :bleach @20:25:07.61 AS.seek 0.82ml @20:25:10.91 delay 3 seconds @20:25:13.93 ARV.dial :waste @20:25:14.51 delay 3 seconds @20:25:17.53 ATV.dial :ARV @20:25:18.36 AS.reconfigure ASconfig @20:25:18.42 AS.seek 0.47ml @20:25:38.82 AFV.select :carrierSolution @20:25:38.94 delay 2 seconds @20:25:40.97 AS.seek 1ml @20:26:11.73 delay 1 second @20:26:12.74 AFV.select :holdingCoil @20:26:12.85 delay 1 second @20:26:13.88 AS.reconfigure ASfast @20:26:13.93 AS.seek 0ml @20:26:41.26 AFV.select :carrierSolution @20:26:41.38 delay 2 seconds @20:26:43.41 AS.reconfigure ASconfig @20:26:43.46 AS.seek 1ml @20:27:41.26 delay 1 second @20:27:42.27 AFV.select :holdingCoil @20:27:42.38 delay 1 second @20:27:43.41 AS.reconfigure ASfast @20:27:43.46 AS.seek 0ml @20:28:10.79 AFV.select :carrierSolution @20:28:10.91 delay 2 seconds @20:28:12.94 AS.reconfigure ASconfig @20:28:12.99 AS.seek 1ml @20:29:10.74 delay 1 second @20:29:11.75 AFV.select :holdingCoil @20:29:11.86 delay 1 second @20:29:12.89 AS.reconfigure ASfast @20:29:12.94 AS.seek 0ml @20:29:40.28 ARV.dial between carrier and air1 @20:29:41.37 ATV.dial :air3 @20:29:42.58 AS.reconfigure ASslow1 @20:29:42.63 AS.seek 0.01ml @20:29:44.95 delay 2 seconds @20:29:46.96 ATV.dial :bleach @20:29:47.56 AS.seek 0.81ml @20:32:28.71 delay 3 seconds @20:32:31.73 ATV.dial :air3 @20:32:32.26 delay 3 seconds @20:32:35.35 AS.seek 0.835ml @20:32:40.72 delay 3 seconds @20:32:43.74 ATV.dial :bleach @20:32:44.34 AS.seek 0.82ml @20:32:47.63 delay 3 seconds @20:32:50.65 ATV.dial PCR @20:32:51.04 AS.reconfigure ASconfig @20:32:51.09 AS.seek :empty @20:33:38.56 PCR.program :bleachProfile @20:33:38.63 PCR program should complete in < 2:45 @20:33:38.64 PCR.run 10:00 @20:36:46.51 PCR.off @20:36:46.54 AFV.select :carrierSolution @20:36:46.66 delay 2 seconds @20:36:48.69 AS.seek 1ml @20:37:46.51 delay 1 second @20:37:47.52 AFV.select :holdingCoil @20:37:47.63 delay 1 second @20:37:48.66 AS.reconfigure ASfast @20:37:48.71 AS.seek 0ml @20:38:16.04 delay 3 seconds @20:38:19.06 AFV.select :carrierSolution @20:38:19.17 delay 2 seconds @20:38:21.20 AS.reconfigure ASconfig @20:38:21.27 AS.seek 1ml @20:39:19.06 delay 1 second @20:39:20.07 AFV.select :holdingCoil @20:39:20.18 delay 1 second @20:39:21.21 AS.reconfigure ASfast @20:39:21.26 AS.seek 0ml @20:39:48.60 delay 3 seconds @20:39:51.62 AFV.select :carrierSolution @20:39:51.73 delay 2 seconds @20:39:53.76 AS.reconfigure ASconfig @20:39:53.81 AS.seek 1ml @20:40:51.59 delay 1 second @20:40:52.60 AFV.select :holdingCoil @20:40:52.71 delay 1 second @20:40:53.74 AS.reconfigure ASfast @20:40:53.79 AS.seek 0ml @20:41:21.15 delay 3 seconds @20:41:24.17 AFV.select :carrierSolution @20:41:24.28 delay 2 seconds @20:41:26.31 AS.reconfigure ASconfig @20:41:26.37 AS.seek 1ml @20:42:24.12 delay 1 second @20:42:25.13 AFV.select :holdingCoil @20:42:25.24 delay 1 second @20:42:26.27 AS.reconfigure ASfast @20:42:26.32 AS.seek 0ml @20:42:53.68 delay 3 seconds @20:42:56.70 AFV.select :carrierSolution @20:42:56.81 delay 2 seconds @20:42:58.84 AS.reconfigure ASconfig @20:42:58.90 AS.seek 1ml @20:43:56.75 delay 1 second @20:43:57.77 AFV.select :holdingCoil @20:43:57.88 delay 1 second @20:43:58.91 AS.reconfigure ASnorm @20:43:58.96 AS.seek 0ml @20:44:49.69 Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0620h1500ml.pcr @20:44:49.71 Building PCR Slug from ASV with MM3 mix, PP5 primer @20:44:49.73 Priming with carrier @20:44:49.74 ATV.dial ARV @20:44:50.31 ARV.dial :carrier @20:44:50.66 AS.seek 0.5ml @20:45:16.22 AFV.select :carrierSolution @20:45:16.34 delay 2 seconds @20:45:18.37 AS.reconfigure ASconfig @20:45:18.42 AS.seek 1ml @20:45:47.42 delay 1 second @20:45:48.43 AFV.select :holdingCoil @20:45:48.54 delay 1 second @20:45:49.56 ATV.dial :waste @20:45:51.09 AS.reconfigure ASnorm @20:45:51.14 AS.seek :empty @20:46:41.88 AFV.select :carrierSolution @20:46:42.00 delay 2 seconds @20:46:44.03 AS.reconfigure ASconfig @20:46:44.08 AS.seek 0.5ml @20:47:13.15 delay 1 second @20:47:14.17 AFV.select :holdingCoil @20:47:14.28 Building Slug @20:47:14.29 ATV.dial :ARV @20:47:15.78 delay 2 seconds @20:47:17.80 ARV.dial :air1 @20:47:18.78 delay 2 seconds @20:47:20.89 AS.reconfigure ASslow2 @20:47:20.94 AS.seek 0.506ml @20:47:27.12 delay 2 seconds @20:47:29.14 ARV.dial :MM3 @20:47:30.12 delay 2 seconds @20:47:32.15 Adding MM3 @20:47:32.24 AS.seek 0.524ml @20:47:50.48 delay 25 seconds @20:48:15.50 ARV.dial :PP5 @20:48:15.89 Adding PP5 @20:48:15.90 delay 2 seconds @20:48:17.99 AS.seek 0.53ml @20:48:24.28 delay 10 seconds @20:48:34.30 ARV.dial :air3 @20:48:34.67 adding ARV air @20:48:34.68 delay 2 seconds @20:48:36.77 AS.seek 0.536ml @20:48:43.09 delay 5 seconds @20:48:48.11 ARV.dial :carrier @20:48:49.31 delay 7 seconds @20:48:56.40 AS.seek 0.594ml @20:49:54.98 delay 5 seconds @20:50:00.00 ATV.dial :ASV @20:50:00.40 delay 2 seconds @20:50:02.49 AS.seek 0.6ml @20:50:08.75 delay 4 seconds @20:50:12.77 ATV.dial ARV @20:50:13.39 delay 2 seconds @20:50:15.41 ARV.dial :carrier @20:50:15.43 delay 2 seconds @20:50:17.52 AS.seek 0.65ml @20:51:07.90 delay 4 seconds @20:51:11.92 ATV.dial PCR @20:51:12.51 ASV.dial :waste1 @20:51:12.52 ARV.dial :air1 @20:51:12.89 Positioning Slug by 0.324ml @20:51:12.99 AS.seek 0.326ml @20:56:39.30 delay 5 seconds @20:56:44.32 ATV.dial ARV @20:56:45.05 ASV.dial :PCRlock @20:56:45.44 delay 5 seconds @20:56:50.47 Running Bb Profile60C300 thermocycling @20:56:50.48 PCR.program Profile60C300 @20:56:50.62 Profile60C300 should complete in < 1:45:21 @20:56:50.63 PCR.run 2:16:41.25 @22:18:39.84 PCR.off @22:18:41.41 PCR for Bb completed -- closed /var/log/esp/Bb18oct0620h1500ml.pcr @22:18:41.48 Cleaning PCR @22:18:41.49 AFV.select :carrierSolution @22:18:41.61 delay 2 seconds @22:18:43.64 AS.reconfigure ASconfig @22:18:43.69 AS.seek 0.5ml @22:18:53.96 delay 1 second @22:18:54.97 AFV.select :holdingCoil @22:18:55.08 ASV.dial :waste1 @22:18:55.46 ATV.dial :air3 @22:18:56.63 AS.reconfigure ASslow1 @22:18:56.69 AS.seek 0.51ml @22:18:59.02 delay 2 seconds @22:19:01.04 ATV.dial :bleach @22:19:01.64 AS.seek 0.81ml @22:20:02.23 delay 3 seconds @22:20:05.25 ATV.dial :air3 @22:20:05.78 delay 3 seconds @22:20:08.87 AS.seek 0.835ml @22:20:14.21 delay 3 seconds @22:20:17.23 ATV.dial :bleach @22:20:17.83 AS.seek 0.82ml @22:20:21.10 delay 3 seconds @22:20:24.12 ARV.dial :waste @22:20:24.71 delay 3 seconds @22:20:27.73 ATV.dial :ARV @22:20:28.58 AS.reconfigure ASconfig @22:20:28.63 AS.seek 0.47ml @22:20:49.03 AFV.select :carrierSolution @22:20:49.15 delay 2 seconds @22:20:51.18 AS.seek 1ml @22:21:21.93 delay 1 second @22:21:22.94 AFV.select :holdingCoil @22:21:23.05 delay 1 second @22:21:24.08 AS.reconfigure ASfast @22:21:24.13 AS.seek 0ml @22:21:51.45 AFV.select :carrierSolution @22:21:51.57 delay 2 seconds @22:21:53.60 AS.reconfigure ASconfig @22:21:53.65 AS.seek 1ml @22:22:51.40 delay 1 second @22:22:52.41 AFV.select :holdingCoil @22:22:52.52 delay 1 second @22:22:53.55 AS.reconfigure ASfast @22:22:53.60 AS.seek 0ml @22:23:21.52 AFV.select :carrierSolution @22:23:21.64 delay 2 seconds @22:23:23.67 AS.reconfigure ASconfig @22:23:23.72 AS.seek 1ml @22:24:21.51 delay 1 second @22:24:22.52 AFV.select :holdingCoil @22:24:22.63 delay 1 second @22:24:23.66 AS.reconfigure ASfast @22:24:23.71 AS.seek 0ml @22:24:51.03 ARV.dial between carrier and air1 @22:24:51.53 ATV.dial :air3 @22:24:52.74 AS.reconfigure ASslow1 @22:24:52.79 AS.seek 0.01ml @22:24:55.14 delay 2 seconds @22:24:57.15 ATV.dial :bleach @22:24:57.75 AS.seek 0.81ml @22:27:38.89 delay 3 seconds @22:27:41.91 ATV.dial :air3 @22:27:42.43 delay 3 seconds @22:27:45.52 AS.seek 0.835ml @22:27:50.84 delay 3 seconds @22:27:53.86 ATV.dial :bleach @22:27:54.46 AS.seek 0.82ml @22:27:57.75 delay 3 seconds @22:28:00.77 ATV.dial PCR @22:28:01.15 AS.reconfigure ASconfig @22:28:01.20 AS.seek :empty @22:28:48.66 PCR.program :bleachProfile @22:28:48.74 PCR program should complete in < 2:45 @22:28:48.76 PCR.run 10:00 @22:31:56.67 PCR.off @22:31:56.69 AFV.select :carrierSolution @22:31:56.80 delay 2 seconds @22:31:58.83 AS.seek 1ml @22:32:56.66 delay 1 second @22:32:57.67 AFV.select :holdingCoil @22:32:57.78 delay 1 second @22:32:58.81 AS.reconfigure ASfast @22:32:58.86 AS.seek 0ml @22:33:26.20 delay 3 seconds @22:33:29.22 AFV.select :carrierSolution @22:33:29.33 delay 2 seconds @22:33:31.36 AS.reconfigure ASconfig @22:33:31.42 AS.seek 1ml @22:34:29.22 delay 1 second @22:34:30.23 AFV.select :holdingCoil @22:34:30.34 delay 1 second @22:34:31.37 AS.reconfigure ASfast @22:34:31.42 AS.seek 0ml @22:34:58.77 delay 3 seconds @22:35:01.79 AFV.select :carrierSolution @22:35:01.90 delay 2 seconds @22:35:03.93 AS.reconfigure ASconfig @22:35:03.98 AS.seek 1ml @22:36:01.78 delay 1 second @22:36:02.79 AFV.select :holdingCoil @22:36:02.90 delay 1 second @22:36:03.93 AS.reconfigure ASfast @22:36:03.98 AS.seek 0ml @22:36:31.31 delay 3 seconds @22:36:34.33 AFV.select :carrierSolution @22:36:34.44 delay 2 seconds @22:36:36.47 AS.reconfigure ASconfig @22:36:36.53 AS.seek 1ml @22:37:34.33 delay 1 second @22:37:35.34 AFV.select :holdingCoil @22:37:35.45 delay 1 second @22:37:36.48 AS.reconfigure ASfast @22:37:36.53 AS.seek 0ml @22:38:03.88 delay 3 seconds @22:38:06.90 AFV.select :carrierSolution @22:38:07.01 delay 2 seconds @22:38:09.04 AS.reconfigure ASconfig @22:38:09.09 AS.seek 1ml @22:39:06.91 delay 1 second @22:39:07.92 AFV.select :holdingCoil @22:39:08.03 delay 1 second @22:39:09.06 AS.reconfigure ASnorm @22:39:09.11 AS.seek 0ml @22:39:59.86 Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0622h1500ml.pcr @22:39:59.88 Building PCR Slug from ASV with MM1 mix, PP2 primer @22:39:59.89 Priming with carrier @22:39:59.90 ATV.dial ARV @22:40:00.47 ARV.dial :carrier @22:40:00.82 AS.seek 0.5ml @22:40:26.35 AFV.select :carrierSolution @22:40:26.47 delay 2 seconds @22:40:28.50 AS.reconfigure ASconfig @22:40:28.55 AS.seek 1ml @22:40:57.64 delay 1 second @22:40:58.65 AFV.select :holdingCoil @22:40:58.76 delay 1 second @22:40:59.78 ATV.dial :waste @22:41:01.31 AS.reconfigure ASnorm @22:41:01.36 AS.seek :empty @22:41:52.10 AFV.select :carrierSolution @22:41:52.22 delay 2 seconds @22:41:54.85 AS.reconfigure ASconfig @22:41:54.90 AS.seek 0.5ml @22:42:24.00 delay 1 second @22:42:25.01 AFV.select :holdingCoil @22:42:25.12 Building Slug @22:42:25.13 ATV.dial :ARV @22:42:26.74 delay 2 seconds @22:42:28.76 ARV.dial :air1 @22:42:29.15 delay 2 seconds @22:42:31.24 AS.reconfigure ASslow2 @22:42:31.29 AS.seek 0.506ml @22:42:37.50 delay 2 seconds @22:42:39.52 ARV.dial :MM1 @22:42:40.10 delay 2 seconds @22:42:42.13 Adding MM1 @22:42:42.20 AS.seek 0.524ml @22:43:00.58 delay 25 seconds @22:43:25.60 ARV.dial :PP2 @22:43:25.97 Adding PP2 @22:43:25.98 delay 2 seconds @22:43:28.07 AS.seek 0.53ml @22:43:34.27 delay 10 seconds @22:43:44.29 ARV.dial :air2 @22:43:44.66 adding ARV air @22:43:44.67 delay 2 seconds @22:43:46.76 AS.seek 0.536ml @22:43:52.92 delay 5 seconds @22:43:57.95 ARV.dial :carrier @22:43:59.14 delay 7 seconds @22:44:06.23 AS.seek 0.594ml @22:45:05.00 delay 5 seconds @22:45:10.02 ATV.dial :ASV @22:45:10.42 delay 2 seconds @22:45:12.51 AS.seek 0.6ml @22:45:18.97 delay 4 seconds @22:45:22.99 ATV.dial ARV @22:45:23.61 delay 2 seconds @22:45:25.64 ARV.dial :carrier @22:45:25.65 delay 2 seconds @22:45:27.74 AS.seek 0.65ml @22:46:18.53 delay 4 seconds @22:46:22.55 ATV.dial PCR @22:46:23.14 ASV.dial :waste1 @22:46:23.15 ARV.dial :air1 @22:46:23.54 Positioning Slug by 0.324ml @22:46:23.63 AS.seek 0.326ml @22:51:49.68 delay 5 seconds @22:51:54.70 ATV.dial ARV @22:51:55.43 ASV.dial :PCRlock @22:51:55.82 delay 5 seconds @22:52:00.85 Running Ss Profile60C350 thermocycling @22:52:00.86 PCR.program Profile60C350 @22:52:01.01 Profile60C350 should complete in < 1:48:05 @22:52:01.02 PCR.run 2:20:06.25 @00:14:23.13CEST07-Oct-18 PCR.off @00:14:25.18 PCR for Ss completed -- closed /var/log/esp/Ss18oct0622h1500ml.pcr @00:14:25.25 Cleaning PCR @00:14:25.26 AFV.select :carrierSolution @00:14:25.37 delay 2 seconds @00:14:27.40 AS.reconfigure ASconfig @00:14:27.45 AS.seek 0.5ml @00:14:37.77 delay 1 second @00:14:38.78 AFV.select :holdingCoil @00:14:38.89 ASV.dial :waste1 @00:14:39.28 ATV.dial :air3 @00:14:40.47 AS.reconfigure ASslow1 @00:14:40.53 AS.seek 0.51ml @00:14:42.81 delay 2 seconds @00:14:44.83 ATV.dial :bleach @00:14:45.43 AS.seek 0.81ml @00:15:46.00 delay 3 seconds @00:15:49.02 ATV.dial :air3 @00:15:49.55 delay 3 seconds @00:15:52.64 AS.seek 0.835ml @00:15:57.93 delay 3 seconds @00:16:00.95 ATV.dial :bleach @00:16:02.15 AS.seek 0.82ml @00:16:05.47 delay 3 seconds @00:16:08.51 ARV.dial :waste @00:16:09.11 delay 3 seconds @00:16:12.13 ATV.dial :ARV @00:16:12.98 AS.reconfigure ASconfig @00:16:13.03 AS.seek 0.47ml @00:16:33.44 AFV.select :carrierSolution @00:16:33.56 delay 2 seconds @00:16:35.59 AS.seek 1ml @00:17:06.36 delay 1 second @00:17:07.37 AFV.select :holdingCoil @00:17:07.48 delay 1 second @00:17:08.51 AS.reconfigure ASfast @00:17:08.56 AS.seek 0ml @00:17:35.88 AFV.select :carrierSolution @00:17:36.00 delay 2 seconds @00:17:38.03 AS.reconfigure ASconfig @00:17:38.08 AS.seek 1ml @00:18:35.89 delay 1 second @00:18:36.90 AFV.select :holdingCoil @00:18:37.01 delay 1 second @00:18:38.04 AS.reconfigure ASfast @00:18:38.09 AS.seek 0ml @00:19:05.44 AFV.select :carrierSolution @00:19:05.56 delay 2 seconds @00:19:07.59 AS.reconfigure ASconfig @00:19:07.64 AS.seek 1ml @00:20:05.47 delay 1 second @00:20:06.48 AFV.select :holdingCoil @00:20:06.59 delay 1 second @00:20:07.62 AS.reconfigure ASfast @00:20:07.67 AS.seek 0ml @00:20:35.02 ARV.dial between carrier and air1 @00:20:35.52 ATV.dial :air3 @00:20:36.73 AS.reconfigure ASslow1 @00:20:36.78 AS.seek 0.01ml @00:20:39.14 delay 2 seconds @00:20:41.15 ATV.dial :bleach @00:20:41.76 AS.seek 0.81ml @00:23:22.91 delay 3 seconds @00:23:25.93 ATV.dial :air3 @00:23:26.46 delay 3 seconds @00:23:29.55 AS.seek 0.835ml @00:23:34.90 delay 3 seconds @00:23:37.92 ATV.dial :bleach @00:23:38.53 AS.seek 0.82ml @00:23:41.85 delay 3 seconds @00:23:44.87 ATV.dial PCR @00:23:45.27 AS.reconfigure ASconfig @00:23:45.32 AS.seek :empty @00:24:32.77 PCR.program :bleachProfile @00:24:32.83 PCR program should complete in < 2:45 @00:24:32.84 PCR.run 10:00 @00:27:40.72 PCR.off @00:27:40.74 AFV.select :carrierSolution @00:27:40.86 delay 2 seconds @00:27:42.89 AS.seek 1ml @00:28:40.72 delay 1 second @00:28:41.73 AFV.select :holdingCoil @00:28:41.84 delay 1 second @00:28:42.87 AS.reconfigure ASfast @00:28:42.92 AS.seek 0ml @00:29:10.25 delay 3 seconds @00:29:13.27 AFV.select :carrierSolution @00:29:13.38 delay 2 seconds @00:29:15.41 AS.reconfigure ASconfig @00:29:15.46 AS.seek 1ml @00:30:13.24 delay 1 second @00:30:14.25 AFV.select :holdingCoil @00:30:14.36 delay 1 second @00:30:15.39 AS.reconfigure ASfast @00:30:15.44 AS.seek 0ml @00:30:42.79 delay 3 seconds @00:30:45.81 AFV.select :carrierSolution @00:30:45.92 delay 2 seconds @00:30:47.96 AS.reconfigure ASconfig @00:30:48.01 AS.seek 1ml @00:31:45.79 delay 1 second @00:31:46.80 AFV.select :holdingCoil @00:31:46.91 delay 1 second @00:31:47.94 AS.reconfigure ASfast @00:31:47.99 AS.seek 0ml @00:32:15.32 delay 3 seconds @00:32:18.34 AFV.select :carrierSolution @00:32:18.45 delay 2 seconds @00:32:20.48 AS.reconfigure ASconfig @00:32:20.54 AS.seek 1ml @00:33:18.33 delay 1 second @00:33:19.34 AFV.select :holdingCoil @00:33:19.45 delay 1 second @00:33:20.48 AS.reconfigure ASfast @00:33:20.53 AS.seek 0ml @00:33:47.85 delay 3 seconds @00:33:50.87 AFV.select :carrierSolution @00:33:50.98 delay 2 seconds @00:33:53.01 AS.reconfigure ASconfig @00:33:53.06 AS.seek 1ml @00:34:51.49 delay 1 second @00:34:52.50 AFV.select :holdingCoil @00:34:52.61 delay 1 second @00:34:53.64 AS.reconfigure ASnorm @00:34:53.69 AS.seek 0ml @00:35:44.43 Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0700h1500ml.pcr @00:35:44.45 Building PCR Slug from ASV with MM1 mix, PP1 primer @00:35:44.46 Priming with carrier @00:35:44.47 ATV.dial ARV @00:35:45.05 ARV.dial :carrier @00:35:45.39 AS.seek 0.5ml @00:36:10.92 AFV.select :carrierSolution @00:36:11.04 delay 2 seconds @00:36:13.07 AS.reconfigure ASconfig @00:36:13.12 AS.seek 1ml @00:36:42.18 delay 1 second @00:36:43.19 AFV.select :holdingCoil @00:36:43.30 delay 1 second @00:36:44.32 ATV.dial :waste @00:36:45.86 AS.reconfigure ASnorm @00:36:45.92 AS.seek :empty @00:37:36.64 AFV.select :carrierSolution @00:37:36.76 delay 2 seconds @00:37:38.79 AS.reconfigure ASconfig @00:37:38.84 AS.seek 0.5ml @00:38:07.91 delay 1 second @00:38:08.92 AFV.select :holdingCoil @00:38:09.03 Building Slug @00:38:09.04 ATV.dial :ARV @00:38:10.55 delay 2 seconds @00:38:12.57 ARV.dial :air1 @00:38:13.05 delay 2 seconds @00:38:15.13 AS.reconfigure ASslow2 @00:38:15.19 AS.seek 0.506ml @00:38:21.51 delay 2 seconds @00:38:23.53 ARV.dial :MM1 @00:38:24.12 delay 2 seconds @00:38:26.15 Adding MM1 @00:38:26.22 AS.seek 0.524ml @00:38:44.51 delay 25 seconds @00:39:09.53 ARV.dial :PP1 @00:39:09.91 Adding PP1 @00:39:09.92 delay 2 seconds @00:39:12.01 AS.seek 0.53ml @00:39:18.43 delay 10 seconds @00:39:28.45 ARV.dial :air1 @00:39:28.82 adding ARV air @00:39:28.84 delay 2 seconds @00:39:30.92 AS.seek 0.536ml @00:39:37.21 delay 5 seconds @00:39:42.23 ARV.dial :carrier @00:39:42.63 delay 7 seconds @00:39:49.72 AS.seek 0.594ml @00:40:48.59 delay 5 seconds @00:40:53.61 ATV.dial :ASV @00:40:54.01 delay 2 seconds @00:40:56.10 AS.seek 0.6ml @00:41:02.54 delay 4 seconds @00:41:06.56 ATV.dial ARV @00:41:07.09 delay 2 seconds @00:41:09.11 ARV.dial :carrier @00:41:09.13 delay 2 seconds @00:41:11.22 AS.seek 0.65ml @00:42:01.74 delay 4 seconds @00:42:05.76 ATV.dial PCR @00:42:06.34 ASV.dial :waste1 @00:42:06.35 ARV.dial :air1 @00:42:06.75 Positioning Slug by 0.324ml @00:42:06.84 AS.seek 0.326ml @00:47:32.95 delay 5 seconds @00:47:37.97 ATV.dial ARV @00:47:38.72 ASV.dial :PCRlock @00:47:39.12 delay 5 seconds @00:47:44.15 Running IPc ProfileIPc thermocycling @00:47:44.16 PCR.program ProfileIPc @00:47:44.32 ProfileIPc should complete in < 1:50:49 @00:47:44.33 PCR.run 2:23:31.25 @02:11:57.28 PCR.off @02:12:00.13 PCR for IPc completed -- closed /var/log/esp/IPc18oct0700h1500ml.pcr @02:12:00.15 Full MFB Decontamination @02:12:00.16 Decon extraction column and decon AIV @02:12:00.18 SPE.hold 2:46:40,55C @02:12:00.23 ASV.dial :SPEdelivery @02:12:00.82 delay 20 seconds @02:12:20.85 AFV.select :carrierSolution @02:12:20.96 delay 2 seconds @02:12:22.99 AS.reconfigure ASconfig @02:12:23.04 AS.seek 0.5ml @02:12:33.37 delay 1 second @02:12:34.38 AFV.select :holdingCoil @02:12:34.49 ATV.dial :air1 @02:12:36.34 AS.reconfigure ASslow1 @02:12:36.39 AS.seek 0.52ml @02:12:40.68 ATV.dial :ASV @02:12:42.12 ASV.dial :SPErecovery @02:12:42.75 AS.seek 0.62ml @02:13:03.12 ATV.dial :waste @02:13:04.91 AS.reconfigure ASnorm @02:13:04.96 AS.seek :empty @02:13:36.61 AFV.select :holdingCoil @02:13:36.63 ATV.dial :waste @02:13:36.65 AS.reconfigure ASconfig @02:13:36.70 AS.seek 0ml @02:13:36.98 AFV.select :carrierSolution @02:13:37.10 delay 2 seconds @02:13:39.13 AS.seek 0.5ml @02:14:08.16 delay 1 second @02:14:09.17 AFV.select :holdingCoil @02:14:09.28 ATV.dial :air3 @02:14:09.95 AS.reconfigure ASslow1 @02:14:10.00 AS.seek 0.52ml @02:14:14.26 ATV.dial :air3 @02:14:14.35 AS.seek 0.53ml @02:14:16.63 delay 2 seconds @02:14:18.64 ATV.dial :bleach @02:14:19.11 AS.seek 0.71ml @02:14:55.62 delay 3 seconds @02:14:58.64 ATV.dial :air3 @02:14:59.16 delay 3 seconds @02:15:02.25 AS.seek 0.735ml @02:15:07.58 delay 3 seconds @02:15:10.60 ATV.dial :bleach @02:15:11.20 AS.seek 0.72ml @02:15:14.52 delay 3 seconds @02:15:17.55 ATV.dial ASV @02:15:18.52 ASV.dial :SPErecovery @02:15:18.60 AS.seek 0.365ml @02:16:30.26 ATV.dial :waste @02:16:31.93 AS.reconfigure ASnorm @02:16:31.98 AS.seek :empty @02:16:50.75 ATV.dial :air3 @02:16:51.52 AS.reconfigure ASslow1 @02:16:51.57 AS.seek 0.009ml @02:16:53.85 delay 2 seconds @02:16:55.86 ATV.dial :bleach @02:16:56.33 AS.seek 0.579ml @02:18:51.21 delay 3 seconds @02:18:54.23 ATV.dial :air3 @02:18:54.76 delay 3 seconds @02:18:57.85 AS.seek 0.604ml @02:19:03.20 delay 3 seconds @02:19:06.22 ATV.dial :bleach @02:19:06.83 AS.seek 0.589ml @02:19:10.15 delay 3 seconds @02:19:13.17 ATV.dial :ASV @02:19:14.13 ASV.dial :wasteR @02:19:14.58 AS.seek 0ml @02:21:13.34 delay 1:00 @02:22:13.36 ATV.dial ASV @02:22:13.38 AFV.select :carrierSolution @02:22:13.48 delay 2 seconds @02:22:15.51 AS.reconfigure ASconfig @02:22:15.56 AS.seek 1ml @02:23:13.31 delay 1 second @02:23:14.32 AFV.select :holdingCoil @02:23:14.43 delay 1 second @02:23:15.45 ASV.dial :wasteR @02:23:16.05 AS.reconfigure ASslow1 @02:23:16.12 AS.seek 0ml @02:26:37.51 delay 10 seconds @02:26:47.54 ATV.dial :air3 @02:26:48.96 AS.seek 0.01ml @02:26:51.26 delay 2 seconds @02:26:53.27 ATV.dial :bleach @02:26:53.88 AS.seek 0.58ml @02:28:48.78 delay 3 seconds @02:28:51.80 ATV.dial :air3 @02:28:52.32 delay 3 seconds @02:28:55.41 AS.seek 0.605ml @02:29:00.71 delay 3 seconds @02:29:03.73 ATV.dial :bleach @02:29:04.33 AS.seek 0.59ml @02:29:07.64 delay 3 seconds @02:29:10.67 ATV.dial ASV @02:29:11.64 ASV.dial :SPEdelivery @02:29:12.01 AS.seek 0ml @02:31:10.92 delay 1:00 @02:32:10.94 AFV.select :carrierSolution @02:32:11.05 delay 2 seconds @02:32:13.08 AS.reconfigure ASconfig @02:32:13.13 AS.seek 1ml @02:33:10.93 delay 1 second @02:33:11.94 AFV.select :holdingCoil @02:33:12.05 delay 1 second @02:33:13.07 ATV.dial ASV @02:33:13.09 ASV.dial :SPEdelivery @02:33:13.11 AS.reconfigure ASslow1 @02:33:13.15 AS.seek 0ml @02:36:34.56 delay 10 seconds @02:36:44.59 ATV.dial :air2 @02:36:45.37 AS.reconfigure ASslow0 @02:36:45.43 AS.seek 0.8ml @02:38:06.25 delay 3 seconds @02:38:09.27 ATV.dial :ASV @02:38:10.29 ASV.dial :SPEdelivery @02:38:10.31 AS.reconfigure ASslow1 @02:38:10.36 AS.seek 0.03ml @02:40:45.47 delay 20 seconds @02:41:05.50 ATV.dial :waste,via: :air3 @02:41:07.09 AS.reconfigure ASconfig @02:41:07.14 AS.seek :empty @02:41:09.22 SPE.coast @02:41:09.31 Removing Bleach from Mixing Coil @02:41:09.32 AFV.select :carrierSolution @02:41:09.43 delay 2 seconds @02:41:11.46 AS.seek 0.5ml @02:41:40.56 delay 1 second @02:41:41.57 AFV.select :holdingCoil @02:41:41.68 ATV.dial :ASV @02:41:43.23 ASV.dial :SPErecovery @02:41:43.85 AS.reconfigure ASslow1 @02:41:43.90 AS.seek 0.73ml @02:42:30.41 ASV.dial :wasteD @02:42:30.95 AS.reconfigure ASnorm @02:42:31.00 AS.seek 0ml @02:43:08.11 AFV.select :carrierSolution @02:43:08.23 delay 2 seconds @02:43:10.26 AS.reconfigure ASconfig @02:43:10.32 AS.seek 0.5ml @02:43:39.41 delay 1 second @02:43:40.42 AFV.select :holdingCoil @02:43:40.53 ATV.dial ASV @02:43:40.54 ASV.dial :SPErecovery @02:43:41.16 AS.reconfigure ASslow0 @02:43:41.21 AS.seek 0.3ml @02:44:01.63 ATV.dial :air2 @02:44:02.73 AS.reconfigure ASslow1 @02:44:02.78 AS.seek 0.32ml @02:44:07.11 ATV.dial ASV @02:44:08.09 AS.reconfigure ASslow0 @02:44:08.14 AS.seek 0.57ml @02:44:33.61 ASV.dial :wasteD @02:44:34.17 AS.reconfigure ASnorm @02:44:34.22 AS.seek 0ml @02:45:03.29 AFV.select :carrierSolution @02:45:03.41 delay 2 seconds @02:45:05.44 AS.reconfigure ASconfig @02:45:05.49 AS.seek 0.5ml @02:45:34.55 delay 1 second @02:45:35.56 AFV.select :holdingCoil @02:45:35.67 ATV.dial ASV @02:45:35.68 ASV.dial :SPErecovery @02:45:36.30 AS.reconfigure ASslow0 @02:45:36.35 AS.seek 0.3ml @02:45:56.79 ATV.dial :air2 @02:45:57.78 AS.reconfigure ASslow1 @02:45:57.84 AS.seek 0.32ml @02:46:02.80 ATV.dial ASV @02:46:03.80 AS.reconfigure ASslow0 @02:46:03.86 AS.seek 0.57ml @02:46:29.33 ASV.dial :wasteD @02:46:29.89 AS.reconfigure ASnorm @02:46:29.94 AS.seek 0ml @02:46:59.01 ATV.dial :air2 @02:46:59.94 AS.reconfigure ASslow0 @02:47:00.00 AS.seek 0.3ml @02:47:30.52 ATV.dial :ASV @02:47:31.44 ASV.dial :wasteD @02:47:31.46 AS.reconfigure ASconfig @02:47:31.51 AS.seek 0.05ml @02:47:46.19 ATV.dial :waste @02:47:47.92 AS.seek 0ml @02:47:51.08 ASV.dial :SPEdelivery @02:47:52.02 deconLysateINT @02:47:52.03 ATV.dial :air3 @02:47:52.70 AS.reconfigure ASslow1 @02:47:52.75 AS.seek 0.01ml @02:47:55.11 delay 2 seconds @02:47:57.12 ATV.dial :bleach @02:47:57.59 AS.seek 0.96ml @02:51:08.91 delay 3 seconds @02:51:11.93 ATV.dial :air3 @02:51:12.45 delay 3 seconds @02:51:15.54 AS.seek 0.985ml @02:51:20.88 delay 3 seconds @02:51:23.90 ATV.dial :bleach @02:51:24.51 AS.seek 0.97ml @02:51:27.77 delay 3 seconds @02:51:30.80 ATV.dial AIV @02:51:32.06 AIV.dial :coilToMFB @02:51:32.07 AWV.select :waste @02:51:32.09 AFV.select :carrierSolution @02:51:32.20 delay 2 seconds @02:51:34.23 AS.reconfigure ASconfig @02:51:34.28 AS.seek 1ml @02:51:36.36 delay 1 second @02:51:37.37 AFV.select :holdingCoil @02:51:37.48 delay 1 second @02:51:38.51 AS.reconfigure ASslow0 @02:51:38.55 AS.seek :empty @02:53:19.54 AFV.select :carrierSolution @02:53:19.66 delay 2 seconds @02:53:21.69 AS.reconfigure ASconfig @02:53:21.74 AS.seek 1ml @02:54:19.55 delay 1 second @02:54:20.56 AFV.select :holdingCoil @02:54:20.67 delay 1 second @02:54:21.70 AS.reconfigure ASslow0 @02:54:21.74 AS.seek :empty @02:56:02.70 AFV.select :carrierSolution @02:56:02.82 delay 2 seconds @02:56:04.85 AS.reconfigure ASconfig @02:56:04.90 AS.seek 1ml @02:57:02.71 delay 1 second @02:57:03.72 AFV.select :holdingCoil @02:57:03.83 delay 1 second @02:57:04.86 AS.reconfigure ASslow0 @02:57:04.90 AS.seek :empty @02:58:45.90 ATV.dial :air2 @02:58:46.57 AS.reconfigure ASslow1 @02:58:46.62 AS.seek 0.009ml @02:58:48.89 AS.reconfigure ASslow0 @02:58:48.94 AS.seek 1ml @03:00:28.90 ATV.dial AIV @03:00:29.65 AS.seek 0.02ml @03:02:08.51 ATV.dial :waste @03:02:10.15 AS.seek 0ml @03:02:12.46 AWV.select :air @03:02:12.64 Cleaning PCR @03:02:12.65 AFV.select :carrierSolution @03:02:12.76 delay 2 seconds @03:02:14.79 AS.reconfigure ASconfig @03:02:14.84 AS.seek 0.5ml @03:02:43.86 delay 1 second @03:02:44.87 AFV.select :holdingCoil @03:02:44.98 ASV.dial :waste1 @03:02:45.43 ATV.dial :air3 @03:02:46.09 AS.reconfigure ASslow1 @03:02:46.14 AS.seek 0.51ml @03:02:48.41 delay 2 seconds @03:02:50.42 ATV.dial :bleach @03:02:50.90 AS.seek 0.81ml @03:03:51.49 delay 3 seconds @03:03:54.51 ATV.dial :air3 @03:03:55.04 delay 3 seconds @03:03:58.13 AS.seek 0.835ml @03:04:04.04 delay 3 seconds @03:04:07.07 ATV.dial :bleach @03:04:07.67 AS.seek 0.82ml @03:04:10.97 delay 3 seconds @03:04:13.99 ARV.dial :waste @03:04:14.58 delay 3 seconds @03:04:17.60 ATV.dial :ARV @03:04:18.54 AS.reconfigure ASconfig @03:04:18.59 AS.seek 0.47ml @03:04:38.99 AFV.select :carrierSolution @03:04:39.11 delay 2 seconds @03:04:41.14 AS.seek 1ml @03:05:11.94 delay 1 second @03:05:12.96 AFV.select :holdingCoil @03:05:13.07 delay 1 second @03:05:14.10 AS.reconfigure ASfast @03:05:14.15 AS.seek 0ml @03:05:41.50 AFV.select :carrierSolution @03:05:41.62 delay 2 seconds @03:05:43.65 AS.reconfigure ASconfig @03:05:43.70 AS.seek 1ml @03:06:41.51 delay 1 second @03:06:42.52 AFV.select :holdingCoil @03:06:42.63 delay 1 second @03:06:43.66 AS.reconfigure ASfast @03:06:43.71 AS.seek 0ml @03:07:11.04 AFV.select :carrierSolution @03:07:11.16 delay 2 seconds @03:07:13.19 AS.reconfigure ASconfig @03:07:13.24 AS.seek 1ml @03:08:11.04 delay 1 second @03:08:12.05 AFV.select :holdingCoil @03:08:12.16 delay 1 second @03:08:13.19 AS.reconfigure ASfast @03:08:13.24 AS.seek 0ml @03:08:40.57 ARV.dial between carrier and air1 @03:08:41.07 ATV.dial :air3 @03:08:42.26 AS.reconfigure ASslow1 @03:08:42.31 AS.seek 0.01ml @03:08:44.62 delay 2 seconds @03:08:46.63 ATV.dial :bleach @03:08:47.23 AS.seek 0.81ml @03:11:28.41 delay 3 seconds @03:11:31.43 ATV.dial :air3 @03:11:31.96 delay 3 seconds @03:11:35.05 AS.seek 0.835ml @03:11:40.34 delay 3 seconds @03:11:43.36 ATV.dial :bleach @03:11:43.97 AS.seek 0.82ml @03:11:47.27 delay 3 seconds @03:11:50.29 ATV.dial PCR @03:11:50.68 AS.reconfigure ASconfig @03:11:50.73 AS.seek :empty @03:12:38.18 PCR.program :bleachProfile @03:12:38.25 PCR program should complete in < 2:45 @03:12:38.26 PCR.run 10:00 @03:15:58.26 PCR.off @03:15:58.28 AFV.select :carrierSolution @03:15:58.40 delay 2 seconds @03:16:00.43 AS.seek 1ml @03:16:58.26 delay 1 second @03:16:59.27 AFV.select :holdingCoil @03:16:59.38 delay 1 second @03:17:00.41 AS.reconfigure ASfast @03:17:00.46 AS.seek 0ml @03:17:27.81 delay 3 seconds @03:17:30.83 AFV.select :carrierSolution @03:17:30.94 delay 2 seconds @03:17:32.97 AS.reconfigure ASconfig @03:17:33.02 AS.seek 1ml @03:18:30.77 delay 1 second @03:18:31.78 AFV.select :holdingCoil @03:18:31.89 delay 1 second @03:18:32.92 AS.reconfigure ASfast @03:18:32.97 AS.seek 0ml @03:19:00.31 delay 3 seconds @03:19:03.33 AFV.select :carrierSolution @03:19:03.44 delay 2 seconds @03:19:05.47 AS.reconfigure ASconfig @03:19:05.57 AS.seek 1ml @03:20:03.35 delay 1 second @03:20:04.36 AFV.select :holdingCoil @03:20:04.47 delay 1 second @03:20:05.50 AS.reconfigure ASfast @03:20:05.55 AS.seek 0ml @03:20:32.88 delay 3 seconds @03:20:35.90 AFV.select :carrierSolution @03:20:36.01 delay 2 seconds @03:20:38.04 AS.reconfigure ASconfig @03:20:38.09 AS.seek 1ml @03:21:35.88 delay 1 second @03:21:36.89 AFV.select :holdingCoil @03:21:37.00 delay 1 second @03:21:38.03 AS.reconfigure ASfast @03:21:38.08 AS.seek 0ml @03:22:06.03 delay 3 seconds @03:22:09.05 AFV.select :carrierSolution @03:22:09.16 delay 2 seconds @03:22:11.19 AS.reconfigure ASconfig @03:22:11.25 AS.seek 1ml @03:23:09.04 delay 1 second @03:23:10.05 AFV.select :holdingCoil @03:23:10.16 delay 1 second @03:23:11.19 AS.reconfigure ASnorm @03:23:11.24 AS.seek 0ml @03:24:01.97 AFV.select :carrierSolution @03:24:02.08 delay 2 seconds @03:24:04.11 AS.reconfigure ASconfig @03:24:04.16 AS.seek 1ml @03:25:01.92 delay 1 second @03:25:02.93 AFV.select :holdingCoil @03:25:03.04 delay 1 second @03:25:04.06 ATV.dial :waste @03:25:05.20 AS.reconfigure ASnorm @03:25:05.26 AS.seek 0.5ml @03:25:30.82 ATV.dial :waste2 @03:25:32.00 AS.seek 0ml @03:25:57.53 AS.coast @03:25:57.57 SS.coast @03:25:57.61 CC.coast @03:25:57.65 CS.coast @03:25:57.69 PC.coast @03:25:57.74 PS.coast @03:25:57.78 Forearm.coast @03:25:57.82 Elbow.coast @03:25:58.21 Gate.power :core,:OFF @03:25:58.22 Gate.power :raw,:OFF @03:25:58.23 Shutdown @03:25:58.25 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @10:50:52.16 Session Begun @10:50:58.43 -> ESP.wakeup @10:50:58.49 Powering core bus @10:51:03.42 Gate.power :raw,:ON @10:51:04.11 SPE.coast @10:51:04.21 SPE.configure SPEconfig @10:51:04.33 AS.configure ASconfig @10:51:04.46 SS.configure SSconfig @10:51:04.59 CC.configure CCconfig @10:51:04.71 CS.configure CSconfig @10:51:04.84 PC.configure PCconfig @10:51:04.96 PS.configure PSconfig @10:51:05.10 Forearm.configure LooseForearm @10:51:05.22 Elbow.configure LooseElbow @10:51:05.37 SC.configure SCloose @10:51:05.50 SE.configure SEconfig @10:51:05.57 CH.coast @10:51:05.65 CH.configure CHconfig @10:51:05.70 PH.coast @10:51:05.79 PH.configure PHconfig @10:51:05.86 PCR.off @10:51:05.91 PCR.pidtemp= 50,0.1,0,300,25,25 @10:51:05.95 PCR.caltemp= 2.7073,0.0373,0 @10:51:06.00 All dwarf controllers running firmware version 3.75 @10:51:06.14 Valve.reset @10:51:06.33 PCR.off @10:51:06.38 PCR.pidtemp= 50,0.1,0,300,25,25 @10:51:06.43 PCR.caltemp= 2.7073,0.0373,0 @10:51:06.62 Intake.select :close @10:51:06.69 Exhaust.select :close @10:51:06.72 CTV.dial :air @10:51:06.85 PSV.dial :air @10:51:06.99 CSV.dial :waste1 @10:51:07.01 Forearm.reconfigure LooseForearm @10:51:07.14 Forearm.home.seek :retract @10:51:07.23 SSV.dial :spit,avoiding: :exhaust @10:51:07.38 PS.reconfigure PSconfig @10:51:07.45 PS.home.seek 0ml @10:51:08.04 Hand.close @10:51:08.16 Elbow.jog 1500 @10:51:08.30 CS.reconfigure CSfast @10:51:08.34 SS.reconfigure SSconfig @10:51:08.47 SS.home.seek 0ml @10:51:08.52 CS.home.seek 0ml @10:51:08.82 Elbow.reconfigure LooseElbow @10:51:08.89 Elbow.home.seek :clear @10:51:12.30 SE.reconfigure SEconfig @10:51:12.37 SE.home.seek :down @10:51:13.00 SC.reconfigure SCsloppy @10:51:13.07 SC.home.seek :initial @10:51:15.31 Forearm.seek :retract @10:51:15.43 Elbow.seek :hammer @10:51:16.70 Forearm.reconfigure TightForearm @10:51:16.78 Forearm.seek 5 + 350 counts @10:51:17.13 PS.coast @10:51:17.89 Forearm.seek :retract @10:51:19.12 PC.reconfigure PCconfig @10:51:19.14 CC.reconfigure CCconfig @10:51:19.27 PC.home.seek :open @10:51:19.29 CC.home.seek :open @10:51:38.36 -> MFB.ready? @10:51:43.16 -> MFB.ready! @10:51:43.28 Readying the MFB @10:51:43.30 Homing MFB Syringe @10:51:43.32 AFV.select :holdingCoil @10:51:43.53 ATV.dial :air2 @10:51:44.07 AS.reconfigure ASconfig @10:51:44.15 AS.home.seek :home @10:51:49.70 AFV.select :carrierSolution @10:51:49.84 delay 2 seconds @10:51:51.87 AS.seek 1ml @10:52:44.81 delay 1 second @10:52:45.82 AFV.select :holdingCoil @10:52:45.95 delay 1 second @10:52:46.98 ATV.dial :waste @10:52:47.98 AS.reconfigure ASnorm @10:52:48.05 AS.home.seek :empty @10:53:40.59 ATV.dial :waste @10:53:40.61 ARV.dial between carrier and air1 @10:53:40.72 ASV.dial :waste1 @10:53:48.00 -> ATV.ARV @10:53:48.02 ATV.dial ARV @10:53:53.42 -> ARV.air3 @10:53:53.45 ARV.dial :air3 @10:54:08.42 -> AS.pull 0.2, ASslow1 @10:54:08.53 AS.reconfigure ASslow1 @10:54:08.61 AS.seek 0.199ml @10:54:53.39 -> ATV @10:55:00.42 -> ARV.PP5 @10:55:00.45 ARV.dial :PP5 @10:55:03.73 -> ARV @10:55:20.67 -> AS.pull 0.05, ASslow1 @10:55:20.78 AS.seek 0.249ml @10:55:38.05 -> ARV.air3 @10:55:38.08 ARV.dial :air3 @10:55:48.17 -> AS.pull 0.2, ASslow1 @10:55:48.28 AS.seek 0.449ml @10:56:33.80 -> ATV.waste @10:56:33.83 ATV.dial :waste @10:56:40.06 -> AS.empty @10:56:40.09 AS.reconfigure ASconfig @10:56:40.17 AS.seek :empty @10:57:18.11 -> pcrDecon @10:57:18.22 Cleaning PCR @10:57:18.24 AFV.select :carrierSolution @10:57:18.44 delay 2 seconds @10:57:20.47 AS.seek 0.5ml @10:57:49.62 delay 1 second @10:57:50.63 AFV.select :holdingCoil @10:57:50.76 ASV.dial :waste1 @10:57:50.77 ATV.dial :air3 @10:57:51.71 AS.reconfigure ASslow1 @10:57:51.78 AS.seek 0.51ml @10:57:54.07 delay 2 seconds @10:57:56.08 ATV.dial :bleach @10:57:56.57 AS.seek 0.81ml @10:58:57.21 delay 3 seconds @10:59:00.23 ATV.dial :air3 @10:59:00.78 delay 3 seconds @10:59:03.87 AS.seek 0.835ml @10:59:09.31 delay 3 seconds @10:59:12.34 ATV.dial :bleach @10:59:13.05 AS.seek 0.82ml @10:59:16.34 delay 3 seconds @10:59:19.36 ARV.dial :waste @10:59:20.37 delay 3 seconds @10:59:23.39 ATV.dial :ARV @10:59:24.25 AS.reconfigure ASconfig @10:59:24.32 AS.seek 0.47ml @10:59:44.74 AFV.select :carrierSolution @10:59:44.88 delay 2 seconds @10:59:46.91 AS.seek 1ml @11:00:17.68 delay 1 second @11:00:18.69 AFV.select :holdingCoil @11:00:18.83 delay 1 second @11:00:19.86 AS.reconfigure ASfast @11:00:19.94 AS.seek 0ml @11:00:47.30 AFV.select :carrierSolution @11:00:47.44 delay 2 seconds @11:00:49.47 AS.reconfigure ASconfig @11:00:49.54 AS.seek 1ml @11:01:47.40 delay 1 second @11:01:48.41 AFV.select :holdingCoil @11:01:48.55 delay 1 second @11:01:49.58 AS.reconfigure ASfast @11:01:49.65 AS.seek 0ml @11:02:17.00 AFV.select :carrierSolution @11:02:17.14 delay 2 seconds @11:02:19.17 AS.reconfigure ASconfig @11:02:19.24 AS.seek 1ml @11:03:17.06 delay 1 second @11:03:18.07 AFV.select :holdingCoil @11:03:18.20 delay 1 second @11:03:19.23 AS.reconfigure ASfast @11:03:19.30 AS.seek 0ml @11:03:46.67 ARV.dial between carrier and air1 @11:03:47.17 ATV.dial :air3 @11:03:48.38 AS.reconfigure ASslow1 @11:03:48.46 AS.seek 0.01ml @11:03:50.78 delay 2 seconds @11:03:52.79 ATV.dial :bleach @11:03:53.41 AS.seek 0.81ml @11:06:34.59 delay 3 seconds @11:06:37.62 ATV.dial :air3 @11:06:38.15 delay 3 seconds @11:06:41.24 AS.seek 0.835ml @11:06:46.57 delay 3 seconds @11:06:49.59 ATV.dial :bleach @11:06:50.21 AS.seek 0.82ml @11:06:53.54 delay 3 seconds @11:06:56.56 ATV.dial PCR @11:06:56.97 AS.reconfigure ASconfig @11:06:57.04 AS.seek :empty @11:07:44.53 PCR.program :bleachProfile @11:07:44.61 PCR program should complete in < 2:45 @11:07:44.62 PCR.run 10:00 @11:11:16.78 PCR.off @11:11:16.82 AFV.select :carrierSolution @11:11:16.96 delay 2 seconds @11:11:18.99 AS.seek 1ml @11:12:16.84 delay 1 second @11:12:17.85 AFV.select :holdingCoil @11:12:17.98 delay 1 second @11:12:19.01 AS.reconfigure ASfast @11:12:19.08 AS.seek 0ml @11:12:46.45 delay 3 seconds @11:12:49.47 AFV.select :carrierSolution @11:12:49.61 delay 2 seconds @11:12:51.64 AS.reconfigure ASconfig @11:12:51.72 AS.seek 1ml @11:13:49.48 delay 1 second @11:13:50.49 AFV.select :holdingCoil @11:13:50.63 delay 1 second @11:13:51.66 AS.reconfigure ASfast @11:13:51.73 AS.seek 0ml @11:14:19.09 delay 3 seconds @11:14:22.11 AFV.select :carrierSolution @11:14:22.24 delay 2 seconds @11:14:24.27 AS.reconfigure ASconfig @11:14:24.34 AS.seek 1ml @11:15:22.19 delay 1 second @11:15:23.20 AFV.select :holdingCoil @11:15:23.33 delay 1 second @11:15:24.36 AS.reconfigure ASfast @11:15:24.43 AS.seek 0ml @11:15:51.78 delay 3 seconds @11:15:54.80 AFV.select :carrierSolution @11:15:54.93 delay 2 seconds @11:15:56.96 AS.reconfigure ASconfig @11:15:57.03 AS.seek 1ml @11:16:54.83 delay 1 second @11:16:55.84 AFV.select :holdingCoil @11:16:55.97 delay 1 second @11:16:57.00 AS.reconfigure ASfast @11:16:57.07 AS.seek 0ml @11:17:24.45 delay 3 seconds @11:17:27.47 AFV.select :carrierSolution @11:17:27.60 delay 2 seconds @11:17:29.63 AS.reconfigure ASconfig @11:17:29.71 AS.seek 1ml @11:18:27.50 delay 1 second @11:18:28.51 AFV.select :holdingCoil @11:18:28.64 delay 1 second @11:18:29.67 AS.reconfigure ASnorm @11:18:29.74 AS.seek 0ml @11:20:11.51 -> ESP.shutdown @11:20:11.55 AS.coast @11:20:11.58 Asleep / Shutdown @11:20:11.66 SS.coast @11:20:11.71 CC.coast @11:20:11.77 CS.coast @11:20:11.83 PC.coast @11:20:11.89 Forearm.coast @11:20:11.95 Elbow.coast @11:20:12.01 SC.coast @11:20:12.62 SE.coast @11:20:13.04 Gate.power :core,:OFF @11:20:13.06 Gate.power :raw,:OFF @11:20:13.09 Shutdown @11:20:17.55 -> exit @11:20:17.57 Session Ended @14:00:00.04
Powering core bus @14:00:05.07 Gate.power :raw,:ON @14:00:05.75 SPE.coast @14:00:05.82 SPE.configure SPEconfig @14:00:05.92 AS.configure ASconfig @14:00:06.02 SS.configure SSconfig @14:00:06.13 CC.configure CCconfig @14:00:06.23 CS.configure CSconfig @14:00:06.33 PC.configure PCconfig @14:00:06.43 PS.configure PSconfig @14:00:06.55 Forearm.configure LooseForearm @14:00:06.65 Elbow.configure LooseElbow @14:00:06.78 SC.configure SCloose @14:00:06.88 SE.configure SEconfig @14:00:06.93 CH.coast @14:00:06.98 CH.configure CHconfig @14:00:07.02 PH.coast @14:00:07.08 PH.configure PHconfig @14:00:07.12 PCR.off @14:00:07.15 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:07.18 PCR.caltemp= 2.7073,0.0373,0 @14:00:07.20 All dwarf controllers running firmware version 3.75 @14:00:07.32 Valve.reset @14:00:07.49 PCR.off @14:00:07.52 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:07.55 PCR.caltemp= 2.7073,0.0373,0 @14:00:07.78 Intake.select :close @14:00:07.80 Exhaust.select :close @14:00:07.81 CTV.dial :air @14:00:07.91 PSV.dial :air @14:00:07.95 CSV.dial :waste1 @14:00:08.14 SSV.dial :spit,avoiding: :exhaust @14:00:08.18 PS.reconfigure PSconfig @14:00:08.20
Forearm.reconfigure LooseForearm @14:00:08.34 PS.home.seek 0ml @14:00:08.36
Forearm.home.seek :retract @14:00:08.54 CS.reconfigure CSfast @14:00:08.57 SS.reconfigure SSconfig @14:00:08.67 CS.home.seek 0ml @14:00:08.70 SS.home.seek 0ml @14:00:09.12 Hand.close @14:00:09.19
Elbow.jog 1500 @14:00:09.68 Elbow.reconfigure LooseElbow @14:00:09.73 Elbow.home.seek :clear @14:00:14.37 SE.reconfigure SEconfig @14:00:14.41 SE.home.seek :down @14:00:15.13 SC.reconfigure SCsloppy @14:00:15.18 SC.home.seek :initial @14:00:17.48 PS.coast @14:00:19.20
Forearm.seek :retract @14:00:19.28 Elbow.seek :hammer @14:00:20.51 Forearm.reconfigure TightForearm @14:00:20.56 Forearm.seek 5 + 350 counts @14:00:21.59 Forearm.seek :retract @14:00:22.79 CC.reconfigure CCconfig @14:00:22.81 PC.reconfigure PCconfig @14:00:22.91
CC.home.seek :open @14:00:22.93 PC.home.seek :open @14:00:26.50
Phase [phasewithdil] @14:00:26.51 GoodNews.email "At 14:00:26 began in tube 2: phasewithdil\n/home/esp/esp2/mission/18tundeployv2dil.rb:118\n{1 => 21, 2 => 1}\nCan@14:00:26, 13.7C, 69% humidity, 14.3psia, 13.613V, 0.853A, 0.846A avg, 11.61W",Subject: "Started 18tundeployv2dil phase phasewithdil" @14:00:26.55CEST07-Oct-18
==================> STARTED Superphasewdil (phase #4) <=== @14:00:26.56 Starting initialPurge @14:00:26.57 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:00:26.65 Hand.open @14:00:27.47 Elbow.reconfigure TightElbow @14:00:27.52 Elbow.seek FlushPuck::Garage @14:00:29.42 Elbow.reconfigure LooseElbow @14:00:29.52 Forearm.reconfigure LooseForearm @14:00:29.57 Forearm.seek :garage @14:00:30.60 Hand.close @14:00:31.37 Forearm.seek :retract @14:00:32.41 Elbow.reconfigure TightElbow @14:00:32.48 Elbow.seek CC @14:00:35.41 Elbow.reconfigure LooseElbow @14:00:35.53 Forearm.reconfigure TightForearm @14:00:35.58 Forearm.seek CC @14:00:36.85 CC.seek :closed @14:00:41.12 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.97 @14:00:41.19 Hand.open @14:00:41.89 Forearm.reconfigure LooseForearm @14:00:41.93 Forearm.seek :retract @14:00:42.90 Flushing the Collection Valve::Manifold @14:00:43.05 CSV.dial CSR @14:00:44.44 Pulling 5ml of CSR.flush @14:00:44.45 CSR.select :flush @14:00:44.57 CS.reconfigure CSconfig @14:00:44.61 CS.seek 5ml @14:00:58.49 delay 5 seconds @14:01:03.51 CSR.select :air @14:01:03.69 CS.reconfigure CSfast @14:01:03.74 CS.seek 7.501ml @14:01:10.36 CSV.dial :waste @14:01:11.68 CS.reconfigure CSconfig @14:01:11.73 CS.seek 0ml @14:01:31.87 Purging external Intake @14:01:32.00 CSV.dial :flush @14:01:32.48 CS.reconfigure CSfast @14:01:32.53 CS.seek 10ml @14:01:56.21 delay 5 seconds @14:02:01.24 CSV.dial CBV @14:02:01.27
CBV.dial CSV @14:02:02.10 CTV.dial :intake @14:02:02.84 Intake.select :open @14:02:02.97 CS.seek 0ml @14:02:27.49 Intake.select :close @14:02:27.61 CTV.dial :air @14:02:28.39 Flushing upper manifold @14:02:28.53 CSV.dial :flush @14:02:29.82 CS.seek 5ml @14:02:42.18 delay 5 seconds @14:02:47.20 CSV.dial :air @14:02:47.75 CS.seek 7.001ml @14:02:53.40 CSV.dial CBV @14:02:53.42
CBV.dial CSV @14:02:54.50 CTV.dial :air @14:02:54.52 CS.seek 0ml @14:03:11.21 delay 7 seconds @14:03:18.25 CS.seek 3ml @14:03:26.20 CSV.dial :waste @14:03:26.69 CS.seek 0ml @14:03:34.55 CSV.dial :flush @14:03:35.02 CS.seek 3ml @14:03:42.78 delay 5 seconds @14:03:47.80 CSV.dial :air @14:03:48.35 CS.seek 5ml @14:03:53.86 CSV.dial :air @14:03:53.89 CS.seek 0ml @14:04:06.09 delay 7 seconds @14:04:13.13 CS.seek 3ml @14:04:20.86 CSV.dial :waste @14:04:21.62 CS.seek 0ml @14:04:29.43 Garaging FlushPuck in Collection Clamp @14:04:29.51 Elbow.reconfigure TightElbow @14:04:29.56 Elbow.seek CC @14:04:29.82 Elbow.reconfigure LooseElbow @14:04:29.93 Forearm.seek CC @14:04:30.91 Hand.close @14:04:31.58 CC.seek :open @14:04:35.94 Forearm.seek :retract @14:04:36.90 Elbow.reconfigure TightElbow @14:04:36.95 Elbow.seek :garage @14:04:40.03 Elbow.reconfigure LooseElbow @14:04:40.14 Forearm.reconfigure TightForearm @14:04:40.23 Forearm.seek :garage @14:04:41.38 Hand.open @14:04:42.02 Forearm.reconfigure LooseForearm @14:04:42.06 Forearm.seek :retract @14:04:43.20 Starting mfb @14:04:43.22 pcrBAC sampling at most 1500ml, wcr at most 1500ml @14:04:43.26 BACfans.email "Started mfb in tube 2 at 14:04:43CEST07-Oct-18\n{1 => 21, 2 => 1}\nCan@14:00:26, 13.7C, 69% humidity, 14.3psia, 13.613V, 0.853A, 0.846A avg, 11.61W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @14:04:43.35
Loading sh1 puck from tube 2 into Collection Clamp @14:04:43.44 Readying the MFB @14:04:43.51 Homing MFB Syringe @14:04:43.52 AFV.select :holdingCoil @14:04:43.76 ATV.dial :air2 @14:04:43.91
SC.reconfigure SCloose @14:04:44.15 Elbow.reconfigure TightElbow @14:04:44.20 Elbow.seek 2 @14:04:45.32 AS.reconfigure ASconfig @14:04:45.36 AS.home.seek :home @14:04:46.25
Forearm.reconfigure TightForearm @14:04:46.33 Forearm.seek 2 + 700 counts @14:04:47.38 SE.reconfigure SEpick @14:04:47.45 SE.seek beamBreak @14:04:50.43 SE.configure SEconfig @14:04:50.62 Elbow.seek 2 @14:04:50.89 Forearm.seek 2 + 300 counts @14:04:50.95 AFV.select :carrierSolution @14:04:51.06 delay 2 seconds @14:04:51.36
Forearm.reconfigure LooseForearm @14:04:51.41 Hand.close @14:04:52.11 SE.seek :down @14:04:52.37
Forearm.seek :retract @14:04:53.09 AS.seek 1ml @14:04:53.36
Elbow.seek CC @14:04:54.99 SE.coast @14:04:55.13
Elbow.reconfigure LooseElbow @14:04:55.23 Forearm.reconfigure TightForearm @14:04:55.29 Forearm.seek CC @14:04:56.57 CC.seek :closed @14:05:00.84 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.98 @14:05:00.85 Hand.open @14:05:01.52 Forearm.reconfigure LooseForearm @14:05:01.57 Forearm.seek :retract @14:05:02.56 SC.coast @14:05:02.61 collectSample 1500ml w/bubble point of 55psi @14:05:02.62 Priming 7ml @14:05:02.63 Intake.select :close @14:05:02.64 Exhaust.select :close @14:05:02.65 SS.hold @14:05:02.88 Calibrating pressure and temperature sensors @14:05:02.90 CTV.dial :air @14:05:02.92 SSV.dial :spit,avoiding: :exhaust @14:05:02.94 SS.reconfigure SSfast @14:05:02.99 SS.seek 5ml @14:05:05.87 SSV.dial CBV @14:05:05.90
CBV.dial SSV @14:05:07.29 delay 3.8 seconds @14:05:11.23 SSV.dial :spit @14:05:12.59 SS.seek 0ml @14:05:15.33 SSV.dial CBV @14:05:16.70 CTV.dial :intake @14:05:17.48 SSV.dial CBV @14:05:17.51
CBV.dial SSV @14:05:17.52 Allowing 40s for 7.0ml of remaining 7.0ml @14:05:17.53 Intake.select :open @14:05:19.71 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:05:19.73 SS.reconfigure SSinhale.with maxSpeed: 150 @14:05:19.78 SS.seek 7ml @14:05:23.33 Vacuum too high -- waiting up to 40s @14:05:24.69 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:05:24.71 SS.reconfigure SSinhale.with maxSpeed: 135 @14:05:24.76 SS.seek 7ml @14:05:27.51 Vacuum too high -- waiting up to 40s @14:05:29.14 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:05:29.16 SS.reconfigure SSinhale.with maxSpeed: 122 @14:05:29.21 SS.seek 7ml @14:05:30.88 Stroke completed -- waiting for pressure to equalize ... @14:05:32.23 Intake.select :close @14:05:32.39 SSV.dial :exhaust @14:05:33.17 Exhaust.select :open @14:05:33.28 SS.reconfigure SSexhale @14:05:33.33 SS.seek 0ml @14:05:37.06 Exhaust.select :close @14:05:37.18 Sampling 1500.0ml @14:05:37.19 Intake.select :close @14:05:37.20 Exhaust.select :close @14:05:37.22 SS.reconfigure SSconfig @14:05:37.26 SS.hold @14:05:37.49 CTV.dial :intake @14:05:37.51 SSV.dial CBV @14:05:37.54
CBV.dial SSV @14:05:38.35 Allowing 130s for 25.0ml of remaining 1500.0ml @14:05:38.36 Intake.select :open @14:05:40.53 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:05:40.55 SS.reconfigure SSinhale.with maxSpeed: 122 @14:05:40.60 SS.seek 25ml @14:05:44.22 Vacuum too high -- waiting up to 40s @14:05:45.57 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:05:45.59 SS.reconfigure SSinhale.with maxSpeed: 110 @14:05:45.64 SS.seek 25ml @14:05:46.03 delay 1 second @14:05:47.04 AFV.select :holdingCoil @14:05:47.15 delay 1 second @14:05:48.18 ATV.dial :waste @14:05:48.48
Vacuum too high -- waiting up to 40s @14:05:49.21 AS.reconfigure ASnorm @14:05:49.29 AS.home.seek :empty @14:05:49.99
Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:05:50.01 SS.reconfigure SSinhale.with maxSpeed: 99 @14:05:50.06 SS.seek 25ml @14:05:52.85 Vacuum too high -- waiting up to 40s @14:05:54.17 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:05:54.19 SS.reconfigure SSinhale.with maxSpeed: 89 @14:05:54.24 SS.seek 25ml @14:05:57.32 Vacuum too high -- waiting up to 40s @14:05:58.67 SS.reconfigure SSinhale.with maxSpeed: 80 @14:05:58.72 SS.seek 25ml @14:06:03.41 Vacuum too high -- waiting up to 40s @14:06:04.52 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:06:04.54 SS.reconfigure SSinhale.with maxSpeed: 72 @14:06:04.59 SS.seek 25ml @14:06:08.70 Vacuum too high -- waiting up to 40s @14:06:09.81 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:06:09.83 SS.reconfigure SSinhale.with maxSpeed: 65 @14:06:09.88 SS.seek 25ml @14:06:15.08 Vacuum too high -- waiting up to 40s @14:06:16.20 SS.reconfigure SSinhale.with maxSpeed: 59 @14:06:16.25 SS.seek 25ml @14:06:18.27 Stroke completed -- waiting for pressure to equalize ... @14:06:20.24 Intake.select :close @14:06:20.40 SSV.dial :exhaust @14:06:21.20 Exhaust.select :open @14:06:21.31 SS.reconfigure SSexhale @14:06:21.36 SS.seek 0ml @14:06:30.11 Exhaust.select :close @14:06:30.23 SSV.dial CBV @14:06:30.26
CBV.dial SSV @14:06:31.07 Allowing 130s for 25.0ml of remaining 1475.0ml @14:06:31.08 Intake.select :open @14:06:33.25 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:06:33.27 SS.reconfigure SSinhale.with maxSpeed: 59 @14:06:33.32 SS.seek 25ml @14:06:40.58 ATV.dial :waste @14:06:40.60 ARV.dial between carrier and air1 @14:06:40.68 ASV.dial :waste1 @14:06:43.32
Vacuum too high -- waiting up to 40s @14:06:44.14 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:06:44.16 SS.reconfigure SSinhale.with maxSpeed: 53 @14:06:44.21 SS.seek 25ml @14:07:07.80 Stroke completed -- waiting for pressure to equalize ... @14:07:10.37 Intake.select :close @14:07:10.53 SSV.dial :exhaust @14:07:11.33 Exhaust.select :open @14:07:11.45 SS.reconfigure SSexhale @14:07:11.50 SS.seek 0ml @14:07:20.27 Exhaust.select :close @14:07:20.39 SSV.dial CBV @14:07:20.42
CBV.dial SSV @14:07:21.23 Allowing 130s for 25.0ml of remaining 1450.0ml @14:07:21.24 Intake.select :open @14:07:23.41 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:07:23.43 SS.reconfigure SSinhale.with maxSpeed: 53 @14:07:23.48 SS.seek 25ml @14:07:57.53 Stroke completed -- waiting for pressure to equalize ... @14:08:00.12 Intake.select :close @14:08:00.27 SSV.dial :exhaust @14:08:01.60 Exhaust.select :open @14:08:01.71 SS.reconfigure SSexhale @14:08:01.76 SS.seek 0ml @14:08:10.44 Exhaust.select :close @14:08:10.56 SSV.dial CBV @14:08:10.59
CBV.dial SSV @14:08:11.42 Allowing 130s for 25.0ml of remaining 1425.0ml @14:08:11.43 Intake.select :open @14:08:13.62 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:08:13.64 SS.reconfigure SSinhale.with maxSpeed: 53 @14:08:13.69 SS.seek 25ml @14:08:48.00 Stroke completed -- waiting for pressure to equalize ... @14:08:50.61 Intake.select :close @14:08:50.77 SSV.dial :exhaust @14:08:51.56 Exhaust.select :open @14:08:51.67 SS.reconfigure SSexhale @14:08:51.72 SS.seek 0ml @14:09:00.48 Exhaust.select :close @14:09:00.60 SSV.dial CBV @14:09:00.63
CBV.dial SSV @14:09:01.45 Allowing 130s for 25.0ml of remaining 1400.0ml @14:09:01.46 Intake.select :open @14:09:03.63 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:09:03.65 SS.reconfigure SSinhale.with maxSpeed: 53 @14:09:03.70 SS.seek 25ml @14:09:37.92 Stroke completed -- waiting for pressure to equalize ... @14:09:40.52 Intake.select :close @14:09:40.68 SSV.dial :exhaust @14:09:41.48 Exhaust.select :open @14:09:41.59 SS.reconfigure SSexhale @14:09:41.64 SS.seek 0ml @14:09:50.45 Exhaust.select :close @14:09:50.57 SSV.dial CBV @14:09:50.60
CBV.dial SSV @14:09:51.40 Allowing 130s for 25.0ml of remaining 1375.0ml @14:09:51.41 Intake.select :open @14:09:53.58 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:09:53.60 SS.reconfigure SSinhale.with maxSpeed: 53 @14:09:53.65 SS.seek 25ml @14:10:27.86 Stroke completed -- waiting for pressure to equalize ... @14:10:30.46 Intake.select :close @14:10:30.62 SSV.dial :exhaust @14:10:31.41 Exhaust.select :open @14:10:31.53 SS.reconfigure SSexhale @14:10:31.58 SS.seek 0ml @14:10:40.72 Exhaust.select :close @14:10:40.84 SSV.dial CBV @14:10:40.87
CBV.dial SSV @14:10:41.68 Allowing 130s for 25.0ml of remaining 1350.0ml @14:10:41.69 Intake.select :open @14:10:43.87 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:10:43.88 SS.reconfigure SSinhale.with maxSpeed: 53 @14:10:43.93 SS.seek 25ml @14:11:17.44 Stroke completed -- waiting for pressure to equalize ... @14:11:20.03 Intake.select :close @14:11:20.19 SSV.dial :exhaust @14:11:20.97 Exhaust.select :open @14:11:21.09 SS.reconfigure SSexhale @14:11:21.14 SS.seek 0ml @14:11:29.94 Exhaust.select :close @14:11:30.06 SSV.dial CBV @14:11:30.09
CBV.dial SSV @14:11:30.92 Allowing 130s for 25.0ml of remaining 1325.0ml @14:11:30.93 Intake.select :open @14:11:33.10 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:11:33.55 SS.reconfigure SSinhale.with maxSpeed: 53 @14:11:33.69 SS.seek 25ml @14:12:07.89 Stroke completed -- waiting for pressure to equalize ... @14:12:10.50 Intake.select :close @14:12:10.66 SSV.dial :exhaust @14:12:11.45 Exhaust.select :open @14:12:11.56 SS.reconfigure SSexhale @14:12:11.61 SS.seek 0ml @14:12:20.42 Exhaust.select :close @14:12:20.54 SSV.dial CBV @14:12:20.57
CBV.dial SSV @14:12:21.39 Allowing 130s for 25.0ml of remaining 1300.0ml @14:12:21.40 Intake.select :open @14:12:23.58 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:12:23.59 SS.reconfigure SSinhale.with maxSpeed: 53 @14:12:23.65 SS.seek 25ml @14:12:57.91 Stroke completed -- waiting for pressure to equalize ... @14:13:00.52 Intake.select :close @14:13:00.68 SSV.dial :exhaust @14:13:01.46 Exhaust.select :open @14:13:01.57 SS.reconfigure SSexhale @14:13:01.62 SS.seek 0ml @14:13:10.89 Exhaust.select :close @14:13:11.01 SSV.dial CBV @14:13:11.04
CBV.dial SSV @14:13:11.85 Allowing 130s for 25.0ml of remaining 1275.0ml @14:13:11.86 Intake.select :open @14:13:14.04 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:13:14.06 SS.reconfigure SSinhale.with maxSpeed: 53 @14:13:14.11 SS.seek 25ml @14:13:48.22 Stroke completed -- waiting for pressure to equalize ... @14:13:50.81 Intake.select :close @14:13:50.97 SSV.dial :exhaust @14:13:51.78 Exhaust.select :open @14:13:51.89 SS.reconfigure SSexhale @14:13:51.95 SS.seek 0ml @14:14:00.98 Exhaust.select :close @14:14:01.10 SSV.dial CBV @14:14:01.13
CBV.dial SSV @14:14:01.93 Allowing 130s for 25.0ml of remaining 1250.0ml @14:14:01.94 Intake.select :open @14:14:04.11 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:14:04.13 SS.reconfigure SSinhale.with maxSpeed: 53 @14:14:04.18 SS.seek 25ml @14:14:36.85 Vacuum too high -- waiting up to 40s @14:14:37.67 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:14:37.69 SS.reconfigure SSinhale.with maxSpeed: 48 @14:14:37.74 SS.seek 25ml @14:14:39.41 Stroke completed -- waiting for pressure to equalize ... @14:14:41.37 Intake.select :close @14:14:41.52 SSV.dial :exhaust @14:14:42.32 Exhaust.select :open @14:14:42.43 SS.reconfigure SSexhale @14:14:42.48 SS.seek 0ml @14:14:51.73 Exhaust.select :close @14:14:51.85 SSV.dial CBV @14:14:51.88
CBV.dial SSV @14:14:52.73 Allowing 130s for 25.0ml of remaining 1225.0ml @14:14:52.74 Intake.select :open @14:14:54.91 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:14:54.93 SS.reconfigure SSinhale.with maxSpeed: 48 @14:14:54.98 SS.seek 25ml @14:15:32.13 Stroke completed -- waiting for pressure to equalize ... @14:15:34.62 Intake.select :close @14:15:34.78 SSV.dial :exhaust @14:15:35.59 Exhaust.select :open @14:15:35.71 SS.reconfigure SSexhale @14:15:35.75 SS.seek 0ml @14:15:44.89 Exhaust.select :close @14:15:45.01 SSV.dial CBV @14:15:45.04
CBV.dial SSV @14:15:45.84 Allowing 130s for 25.0ml of remaining 1200.0ml @14:15:45.85 Intake.select :open @14:15:48.03 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:15:48.04 SS.reconfigure SSinhale.with maxSpeed: 48 @14:15:48.09 SS.seek 25ml @14:16:24.81 Stroke completed -- waiting for pressure to equalize ... @14:16:26.78 Intake.select :close @14:16:26.94 SSV.dial :exhaust @14:16:27.73 Exhaust.select :open @14:16:27.84 SS.reconfigure SSexhale @14:16:27.89 SS.seek 0ml @14:16:36.91 Exhaust.select :close @14:16:37.03 SSV.dial CBV @14:16:37.06
CBV.dial SSV @14:16:37.89 Allowing 130s for 25.0ml of remaining 1175.0ml @14:16:37.90 Intake.select :open @14:16:40.08 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:16:40.09 SS.reconfigure SSinhale.with maxSpeed: 48 @14:16:40.14 SS.seek 25ml @14:17:17.27 Stroke completed -- waiting for pressure to equalize ... @14:17:19.86 Intake.select :close @14:17:20.02 SSV.dial :exhaust @14:17:20.82 Exhaust.select :open @14:17:20.93 SS.reconfigure SSexhale @14:17:20.98 SS.seek 0ml @14:17:29.81 Exhaust.select :close @14:17:29.93 SSV.dial CBV @14:17:29.96
CBV.dial SSV @14:17:30.76 Allowing 130s for 25.0ml of remaining 1150.0ml @14:17:30.77 Intake.select :open @14:17:32.95 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:17:32.96 SS.reconfigure SSinhale.with maxSpeed: 48 @14:17:33.01 SS.seek 25ml @14:18:10.55 Stroke completed -- waiting for pressure to equalize ... @14:18:13.13 Intake.select :close @14:18:13.29 SSV.dial :exhaust @14:18:14.07 Exhaust.select :open @14:18:14.18 SS.reconfigure SSexhale @14:18:14.23 SS.seek 0ml @14:18:23.42 Exhaust.select :close @14:18:23.54 SSV.dial CBV @14:18:23.57
CBV.dial SSV @14:18:24.39 Allowing 130s for 25.0ml of remaining 1125.0ml @14:18:24.40 Intake.select :open @14:18:26.57 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:18:26.59 SS.reconfigure SSinhale.with maxSpeed: 48 @14:18:26.64 SS.seek 25ml @14:19:03.38 Stroke completed -- waiting for pressure to equalize ... @14:19:05.97 Intake.select :close @14:19:06.13 SSV.dial :exhaust @14:19:06.92 Exhaust.select :open @14:19:07.04 SS.reconfigure SSexhale @14:19:07.10 SS.seek 0ml @14:19:16.69 Exhaust.select :close @14:19:16.81 SSV.dial CBV @14:19:16.84
CBV.dial SSV @14:19:17.65 Allowing 130s for 25.0ml of remaining 1100.0ml @14:19:17.66 Intake.select :open @14:19:19.83 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:19:19.85 SS.reconfigure SSinhale.with maxSpeed: 48 @14:19:19.90 SS.seek 25ml @14:19:57.06 Stroke completed -- waiting for pressure to equalize ... @14:19:59.66 Intake.select :close @14:19:59.82 SSV.dial :exhaust @14:20:00.60 Exhaust.select :open @14:20:00.71 SS.reconfigure SSexhale @14:20:00.76 SS.seek 0ml @14:20:09.80 Exhaust.select :close @14:20:09.92 SSV.dial CBV @14:20:09.95
CBV.dial SSV @14:20:10.78 Allowing 130s for 25.0ml of remaining 1075.0ml @14:20:10.79 Intake.select :open @14:20:12.98 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:20:12.99 SS.reconfigure SSinhale.with maxSpeed: 48 @14:20:13.04 SS.seek 25ml @14:20:50.30 Stroke completed -- waiting for pressure to equalize ... @14:20:52.88 Intake.select :close @14:20:53.04 SSV.dial :exhaust @14:20:53.84 Exhaust.select :open @14:20:53.95 SS.reconfigure SSexhale @14:20:54.00 SS.seek 0ml @14:21:03.14 Exhaust.select :close @14:21:03.26 SSV.dial CBV @14:21:03.29
CBV.dial SSV @14:21:04.09 Allowing 130s for 25.0ml of remaining 1050.0ml @14:21:04.10 Intake.select :open @14:21:06.27 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:21:06.29 SS.reconfigure SSinhale.with maxSpeed: 48 @14:21:06.34 SS.seek 25ml @14:21:43.19 Stroke completed -- waiting for pressure to equalize ... @14:21:45.76 Intake.select :close @14:21:45.92 SSV.dial :exhaust @14:21:46.73 Exhaust.select :open @14:21:46.84 SS.reconfigure SSexhale @14:21:46.90 SS.seek 0ml @14:21:55.67 Exhaust.select :close @14:21:55.79 SSV.dial CBV @14:21:55.82
CBV.dial SSV @14:21:56.62 Allowing 130s for 25.0ml of remaining 1025.0ml @14:21:56.63 Intake.select :open @14:21:58.81 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:21:58.82 SS.reconfigure SSinhale.with maxSpeed: 48 @14:21:58.87 SS.seek 25ml @14:22:36.34 Stroke completed -- waiting for pressure to equalize ... @14:22:38.92 Intake.select :close @14:22:39.07 SSV.dial :exhaust @14:22:39.87 Exhaust.select :open @14:22:39.98 SS.reconfigure SSexhale @14:22:40.03 SS.seek 0ml @14:22:49.09 Exhaust.select :close @14:22:49.21 SSV.dial CBV @14:22:49.24
CBV.dial SSV @14:22:50.06 Allowing 130s for 25.0ml of remaining 1000.0ml @14:22:50.07 Intake.select :open @14:22:52.24 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:22:52.26 SS.reconfigure SSinhale.with maxSpeed: 48 @14:22:52.31 SS.seek 25ml @14:23:29.45 Stroke completed -- waiting for pressure to equalize ... @14:23:31.95 Intake.select :close @14:23:32.11 SSV.dial :exhaust @14:23:32.92 Exhaust.select :open @14:23:33.03 SS.reconfigure SSexhale @14:23:33.08 SS.seek 0ml @14:23:42.11 Exhaust.select :close @14:23:42.23 SSV.dial CBV @14:23:42.26
CBV.dial SSV @14:23:43.07 Allowing 130s for 25.0ml of remaining 975.0ml @14:23:43.08 Intake.select :open @14:23:45.25 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:23:45.27 SS.reconfigure SSinhale.with maxSpeed: 48 @14:23:45.32 SS.seek 25ml @14:24:22.44 Stroke completed -- waiting for pressure to equalize ... @14:24:25.04 Intake.select :close @14:24:25.20 SSV.dial :exhaust @14:24:25.99 Exhaust.select :open @14:24:26.10 SS.reconfigure SSexhale @14:24:26.15 SS.seek 0ml @14:24:35.23 Exhaust.select :close @14:24:35.35 SSV.dial CBV @14:24:35.38
CBV.dial SSV @14:24:36.20 Allowing 130s for 25.0ml of remaining 950.0ml @14:24:36.21 Intake.select :open @14:24:38.38 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:24:38.40 SS.reconfigure SSinhale.with maxSpeed: 48 @14:24:38.45 SS.seek 25ml @14:25:15.75 Stroke completed -- waiting for pressure to equalize ... @14:25:18.34 Intake.select :close @14:25:18.50 SSV.dial :exhaust @14:25:19.31 Exhaust.select :open @14:25:19.42 SS.reconfigure SSexhale @14:25:19.48 SS.seek 0ml @14:25:28.67 Exhaust.select :close @14:25:28.79 SSV.dial CBV @14:25:28.82
CBV.dial SSV @14:25:29.62 Allowing 130s for 25.0ml of remaining 925.0ml @14:25:29.63 Intake.select :open @14:25:31.81 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:25:31.82 SS.reconfigure SSinhale.with maxSpeed: 48 @14:25:31.87 SS.seek 25ml @14:26:08.97 Stroke completed -- waiting for pressure to equalize ... @14:26:11.55 Intake.select :close @14:26:11.71 SSV.dial :exhaust @14:26:12.49 Exhaust.select :open @14:26:12.60 SS.reconfigure SSexhale @14:26:12.66 SS.seek 0ml @14:26:21.66 Exhaust.select :close @14:26:21.78 SSV.dial CBV @14:26:21.81
CBV.dial SSV @14:26:22.64 Allowing 130s for 25.0ml of remaining 900.0ml @14:26:22.65 Intake.select :open @14:26:24.82 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:26:24.84 SS.reconfigure SSinhale.with maxSpeed: 48 @14:26:24.89 SS.seek 25ml @14:27:02.14 Stroke completed -- waiting for pressure to equalize ... @14:27:04.73 Intake.select :close @14:27:04.89 SSV.dial :exhaust @14:27:05.69 Exhaust.select :open @14:27:05.81 SS.reconfigure SSexhale @14:27:05.86 SS.seek 0ml @14:27:15.16 Exhaust.select :close @14:27:15.28 SSV.dial CBV @14:27:15.31
CBV.dial SSV @14:27:16.12 Allowing 130s for 25.0ml of remaining 875.0ml @14:27:16.13 Intake.select :open @14:27:18.30 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:27:18.32 SS.reconfigure SSinhale.with maxSpeed: 48 @14:27:18.37 SS.seek 25ml @14:27:55.95 Stroke completed -- waiting for pressure to equalize ... @14:27:58.57 Intake.select :close @14:27:58.73 SSV.dial :exhaust @14:27:59.53 Exhaust.select :open @14:27:59.64 SS.reconfigure SSexhale @14:27:59.69 SS.seek 0ml @14:28:08.86 Exhaust.select :close @14:28:08.98 SSV.dial CBV @14:28:09.01
CBV.dial SSV @14:28:09.82 Allowing 130s for 25.0ml of remaining 850.0ml @14:28:09.83 Intake.select :open @14:28:12.01 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:28:12.02 SS.reconfigure SSinhale.with maxSpeed: 48 @14:28:12.07 SS.seek 25ml @14:28:48.97 Vacuum too high -- waiting up to 40s @14:28:49.81 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:28:49.83 SS.reconfigure SSinhale.with maxSpeed: 43 @14:28:49.88 SS.seek 25ml @14:28:50.66 Stroke completed -- waiting for pressure to equalize ... @14:28:52.64 Intake.select :close @14:28:52.80 SSV.dial :exhaust @14:28:53.58 Exhaust.select :open @14:28:53.70 SS.reconfigure SSexhale @14:28:53.75 SS.seek 0ml @14:29:02.59 Exhaust.select :close @14:29:02.71 SSV.dial CBV @14:29:02.74
CBV.dial SSV @14:29:03.56 Allowing 130s for 25.0ml of remaining 825.0ml @14:29:03.57 Intake.select :open @14:29:05.75 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:29:05.76 SS.reconfigure SSinhale.with maxSpeed: 43 @14:29:05.81 SS.seek 25ml @14:29:47.56 Stroke completed -- waiting for pressure to equalize ... @14:29:50.14 Intake.select :close @14:29:50.30 SSV.dial :exhaust @14:29:51.10 Exhaust.select :open @14:29:51.21 SS.reconfigure SSexhale @14:29:51.26 SS.seek 0ml @14:30:00.44 Exhaust.select :close @14:30:00.56 SSV.dial CBV @14:30:00.59
CBV.dial SSV @14:30:01.40 Allowing 130s for 25.0ml of remaining 800.0ml @14:30:01.41 Intake.select :open @14:30:03.58 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:30:03.60 SS.reconfigure SSinhale.with maxSpeed: 43 @14:30:03.65 SS.seek 25ml @14:30:45.06 Stroke completed -- waiting for pressure to equalize ... @14:30:47.02 Intake.select :close @14:30:47.17 SSV.dial :exhaust @14:30:47.96 Exhaust.select :open @14:30:48.07 SS.reconfigure SSexhale @14:30:48.12 SS.seek 0ml @14:30:57.45 Exhaust.select :close @14:30:57.57 SSV.dial CBV @14:30:57.60
CBV.dial SSV @14:30:58.95 Allowing 130s for 25.0ml of remaining 775.0ml @14:30:58.96 Intake.select :open @14:31:01.14 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:31:01.15 SS.reconfigure SSinhale.with maxSpeed: 43 @14:31:01.20 SS.seek 25ml @14:31:42.58 Stroke completed -- waiting for pressure to equalize ... @14:31:45.19 Intake.select :close @14:31:45.35 SSV.dial :exhaust @14:31:46.15 Exhaust.select :open @14:31:46.26 SS.reconfigure SSexhale @14:31:46.31 SS.seek 0ml @14:31:55.31 Exhaust.select :close @14:31:55.43 SSV.dial CBV @14:31:55.46
CBV.dial SSV @14:31:56.26 Allowing 130s for 25.0ml of remaining 750.0ml @14:31:56.27 Intake.select :open @14:31:58.45 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:31:58.46 SS.reconfigure SSinhale.with maxSpeed: 43 @14:31:58.51 SS.seek 25ml @14:32:39.80 Stroke completed -- waiting for pressure to equalize ... @14:32:42.41 Intake.select :close @14:32:42.57 SSV.dial :exhaust @14:32:43.35 Exhaust.select :open @14:32:43.46 SS.reconfigure SSexhale @14:32:43.51 SS.seek 0ml @14:32:52.22 Exhaust.select :close @14:32:52.34 SSV.dial CBV @14:32:52.37
CBV.dial SSV @14:32:53.20 Allowing 130s for 25.0ml of remaining 725.0ml @14:32:53.21 Intake.select :open @14:32:55.38 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:32:55.40 SS.reconfigure SSinhale.with maxSpeed: 43 @14:32:55.45 SS.seek 25ml @14:33:37.14 Stroke completed -- waiting for pressure to equalize ... @14:33:39.73 Intake.select :close @14:33:39.89 SSV.dial :exhaust @14:33:40.70 Exhaust.select :open @14:33:40.81 SS.reconfigure SSexhale @14:33:40.86 SS.seek 0ml @14:33:50.09 Exhaust.select :close @14:33:50.21 SSV.dial CBV @14:33:50.24
CBV.dial SSV @14:33:51.04 Allowing 130s for 25.0ml of remaining 700.0ml @14:33:51.05 Intake.select :open @14:33:53.22 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:33:53.24 SS.reconfigure SSinhale.with maxSpeed: 43 @14:33:53.29 SS.seek 25ml @14:34:34.37 Stroke completed -- waiting for pressure to equalize ... @14:34:36.94 Intake.select :close @14:34:37.09 SSV.dial :exhaust @14:34:37.88 Exhaust.select :open @14:34:37.99 SS.reconfigure SSexhale @14:34:38.05 SS.seek 0ml @14:34:47.09 Exhaust.select :close @14:34:47.21 SSV.dial CBV @14:34:47.24
CBV.dial SSV @14:34:48.06 Allowing 130s for 25.0ml of remaining 675.0ml @14:34:48.07 Intake.select :open @14:34:50.25 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:34:50.27 SS.reconfigure SSinhale.with maxSpeed: 43 @14:34:50.32 SS.seek 25ml @14:35:31.64 Stroke completed -- waiting for pressure to equalize ... @14:35:34.12 Intake.select :close @14:35:34.28 SSV.dial :exhaust @14:35:35.09 Exhaust.select :open @14:35:35.21 SS.reconfigure SSexhale @14:35:35.25 SS.seek 0ml @14:35:44.36 Exhaust.select :close @14:35:44.48 SSV.dial CBV @14:35:44.51
CBV.dial SSV @14:35:45.32 Allowing 130s for 25.0ml of remaining 650.0ml @14:35:45.33 Intake.select :open @14:35:47.51 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:35:47.52 SS.reconfigure SSinhale.with maxSpeed: 43 @14:35:47.58 SS.seek 25ml @14:36:28.94 Stroke completed -- waiting for pressure to equalize ... @14:36:31.56 Intake.select :close @14:36:31.72 SSV.dial :exhaust @14:36:32.51 Exhaust.select :open @14:36:32.62 SS.reconfigure SSexhale @14:36:32.67 SS.seek 0ml @14:36:41.78 Exhaust.select :close @14:36:41.90 SSV.dial CBV @14:36:41.93
CBV.dial SSV @14:36:42.74 Allowing 130s for 25.0ml of remaining 625.0ml @14:36:42.75 Intake.select :open @14:36:44.92 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:36:44.94 SS.reconfigure SSinhale.with maxSpeed: 43 @14:36:44.99 SS.seek 25ml @14:37:26.19 Stroke completed -- waiting for pressure to equalize ... @14:37:28.78 Intake.select :close @14:37:28.94 SSV.dial :exhaust @14:37:29.73 Exhaust.select :open @14:37:29.85 SS.reconfigure SSexhale @14:37:29.90 SS.seek 0ml @14:37:38.69 Exhaust.select :close @14:37:38.81 SSV.dial CBV @14:37:38.84
CBV.dial SSV @14:37:39.65 Allowing 130s for 25.0ml of remaining 600.0ml @14:37:39.66 Intake.select :open @14:37:41.83 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:37:41.85 SS.reconfigure SSinhale.with maxSpeed: 43 @14:37:41.90 SS.seek 25ml @14:38:23.61 Stroke completed -- waiting for pressure to equalize ... @14:38:26.17 Intake.select :close @14:38:26.32 SSV.dial :exhaust @14:38:27.12 Exhaust.select :open @14:38:27.23 SS.reconfigure SSexhale @14:38:27.28 SS.seek 0ml @14:38:36.45 Exhaust.select :close @14:38:36.57 SSV.dial CBV @14:38:36.60
CBV.dial SSV @14:38:37.42 Allowing 130s for 25.0ml of remaining 575.0ml @14:38:37.43 Intake.select :open @14:38:39.60 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:38:39.62 SS.reconfigure SSinhale.with maxSpeed: 43 @14:38:39.67 SS.seek 25ml @14:39:20.97 Stroke completed -- waiting for pressure to equalize ... @14:39:24.17 Intake.select :close @14:39:24.32 SSV.dial :exhaust @14:39:25.13 Exhaust.select :open @14:39:25.24 SS.reconfigure SSexhale @14:39:25.30 SS.seek 0ml @14:39:34.52 Exhaust.select :close @14:39:35.16 SSV.dial CBV @14:39:35.19
CBV.dial SSV @14:39:35.99 Allowing 130s for 25.0ml of remaining 550.0ml @14:39:36.00 Intake.select :open @14:39:38.17 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:39:38.19 SS.reconfigure SSinhale.with maxSpeed: 43 @14:39:38.24 SS.seek 25ml @14:40:19.37 Stroke completed -- waiting for pressure to equalize ... @14:40:22.59 Intake.select :close @14:40:22.75 SSV.dial :exhaust @14:40:23.54 Exhaust.select :open @14:40:23.65 SS.reconfigure SSexhale @14:40:23.70 SS.seek 0ml @14:40:33.00 Exhaust.select :close @14:40:33.12 SSV.dial CBV @14:40:33.15
CBV.dial SSV @14:40:33.96 Allowing 130s for 25.0ml of remaining 525.0ml @14:40:33.97 Intake.select :open @14:40:36.15 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:40:36.16 SS.reconfigure SSinhale.with maxSpeed: 43 @14:40:36.21 SS.seek 25ml @14:41:17.30 Stroke completed -- waiting for pressure to equalize ... @14:41:20.54 Intake.select :close @14:41:20.70 SSV.dial :exhaust @14:41:21.51 Exhaust.select :open @14:41:21.62 SS.reconfigure SSexhale @14:41:21.67 SS.seek 0ml @14:41:30.69 Exhaust.select :close @14:41:30.81 SSV.dial CBV @14:41:30.84
CBV.dial SSV @14:41:31.65 Allowing 130s for 25.0ml of remaining 500.0ml @14:41:31.66 Intake.select :open @14:41:33.85 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:41:33.87 SS.reconfigure SSinhale.with maxSpeed: 43 @14:41:33.93 SS.seek 25ml @14:42:06.86 Vacuum too high -- waiting up to 40s @14:42:07.68 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:42:07.70 SS.reconfigure SSinhale.with maxSpeed: 39 @14:42:07.75 SS.seek 25ml @14:42:17.03 Stroke completed -- waiting for pressure to equalize ... @14:42:19.61 Intake.select :close @14:42:19.77 SSV.dial :exhaust @14:42:20.56 Exhaust.select :open @14:42:20.67 SS.reconfigure SSexhale @14:42:20.72 SS.seek 0ml @14:42:29.92 Exhaust.select :close @14:42:30.04 SSV.dial CBV @14:42:30.07
CBV.dial SSV @14:42:30.88 Allowing 130s for 25.0ml of remaining 475.0ml @14:42:30.89 Intake.select :open @14:42:33.06 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:42:33.08 SS.reconfigure SSinhale.with maxSpeed: 39 @14:42:33.13 SS.seek 25ml @14:43:18.50 Stroke completed -- waiting for pressure to equalize ... @14:43:21.07 Intake.select :close @14:43:21.23 SSV.dial :exhaust @14:43:22.03 Exhaust.select :open @14:43:22.65 SS.reconfigure SSexhale @14:43:22.72 SS.seek 0ml @14:43:31.80 Exhaust.select :close @14:43:31.92 SSV.dial CBV @14:43:31.95
CBV.dial SSV @14:43:32.76 Allowing 130s for 25.0ml of remaining 450.0ml @14:43:32.77 Intake.select :open @14:43:34.96 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:43:34.97 SS.reconfigure SSinhale.with maxSpeed: 39 @14:43:35.02 SS.seek 25ml @14:44:20.30 Stroke completed -- waiting for pressure to equalize ... @14:44:22.91 Intake.select :close @14:44:23.07 SSV.dial :exhaust @14:44:23.84 Exhaust.select :open @14:44:23.96 SS.reconfigure SSexhale @14:44:24.01 SS.seek 0ml @14:44:33.11 Exhaust.select :close @14:44:33.23 SSV.dial CBV @14:44:33.26
CBV.dial SSV @14:44:34.07 Allowing 130s for 25.0ml of remaining 425.0ml @14:44:34.08 Intake.select :open @14:44:36.26 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:44:36.27 SS.reconfigure SSinhale.with maxSpeed: 39 @14:44:36.32 SS.seek 25ml @14:45:21.59 Stroke completed -- waiting for pressure to equalize ... @14:45:24.19 Intake.select :close @14:45:24.35 SSV.dial :exhaust @14:45:25.17 Exhaust.select :open @14:45:25.28 SS.reconfigure SSexhale @14:45:25.33 SS.seek 0ml @14:45:34.45 Exhaust.select :close @14:45:34.57 SSV.dial CBV @14:45:34.60
CBV.dial SSV @14:45:35.40 Allowing 130s for 25.0ml of remaining 400.0ml @14:45:35.41 Intake.select :open @14:45:37.58 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:45:37.60 SS.reconfigure SSinhale.with maxSpeed: 39 @14:45:37.65 SS.seek 25ml @14:46:23.14 Stroke completed -- waiting for pressure to equalize ... @14:46:25.81 Intake.select :close @14:46:25.97 SSV.dial :exhaust @14:46:26.75 Exhaust.select :open @14:46:26.87 SS.reconfigure SSexhale @14:46:26.92 SS.seek 0ml @14:46:35.64 Exhaust.select :close @14:46:35.76 SSV.dial CBV @14:46:35.80
CBV.dial SSV @14:46:36.60 Allowing 130s for 25.0ml of remaining 375.0ml @14:46:36.61 Intake.select :open @14:46:38.78 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:46:38.80 SS.reconfigure SSinhale.with maxSpeed: 39 @14:46:38.85 SS.seek 25ml @14:47:24.58 Stroke completed -- waiting for pressure to equalize ... @14:47:27.79 Intake.select :close @14:47:27.95 SSV.dial :exhaust @14:47:28.76 Exhaust.select :open @14:47:28.87 SS.reconfigure SSexhale @14:47:28.92 SS.seek 0ml @14:47:37.97 Exhaust.select :close @14:47:38.09 SSV.dial CBV @14:47:38.12
CBV.dial SSV @14:47:38.92 Allowing 130s for 25.0ml of remaining 350.0ml @14:47:38.93 Intake.select :open @14:47:41.10 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:47:41.55 SS.reconfigure SSinhale.with maxSpeed: 39 @14:47:41.69 SS.seek 25ml @14:48:26.83 Stroke completed -- waiting for pressure to equalize ... @14:48:30.06 Intake.select :close @14:48:30.22 SSV.dial :exhaust @14:48:31.01 Exhaust.select :open @14:48:31.12 SS.reconfigure SSexhale @14:48:31.17 SS.seek 0ml @14:48:39.89 Exhaust.select :close @14:48:40.01 SSV.dial CBV @14:48:40.04
CBV.dial SSV @14:48:40.85 Allowing 130s for 25.0ml of remaining 325.0ml @14:48:40.86 Intake.select :open @14:48:43.04 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:48:43.05 SS.reconfigure SSinhale.with maxSpeed: 39 @14:48:43.10 SS.seek 25ml @14:49:28.92 Stroke completed -- waiting for pressure to equalize ... @14:49:32.16 Intake.select :close @14:49:32.32 SSV.dial :exhaust @14:49:33.10 Exhaust.select :open @14:49:33.21 SS.reconfigure SSexhale @14:49:33.26 SS.seek 0ml @14:49:42.28 Exhaust.select :close @14:49:42.40 SSV.dial CBV @14:49:42.43
CBV.dial SSV @14:49:43.24 Allowing 130s for 25.0ml of remaining 300.0ml @14:49:43.25 Intake.select :open @14:49:45.42 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:49:45.44 SS.reconfigure SSinhale.with maxSpeed: 39 @14:49:45.49 SS.seek 25ml @14:50:30.87 Stroke completed -- waiting for pressure to equalize ... @14:50:34.08 Intake.select :close @14:50:34.24 SSV.dial :exhaust @14:50:35.03 Exhaust.select :open @14:50:35.15 SS.reconfigure SSexhale @14:50:35.20 SS.seek 0ml @14:50:43.97 Exhaust.select :close @14:50:44.09 SSV.dial CBV @14:50:44.12
CBV.dial SSV @14:50:44.93 Allowing 130s for 25.0ml of remaining 275.0ml @14:50:44.94 Intake.select :open @14:50:47.12 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:50:47.13 SS.reconfigure SSinhale.with maxSpeed: 39 @14:50:47.19 SS.seek 25ml @14:51:32.91 Stroke completed -- waiting for pressure to equalize ... @14:51:36.10 Intake.select :close @14:51:36.25 SSV.dial :exhaust @14:51:37.05 Exhaust.select :open @14:51:37.17 SS.reconfigure SSexhale @14:51:37.22 SS.seek 0ml @14:51:46.41 Exhaust.select :close @14:51:46.53 SSV.dial CBV @14:51:46.56
CBV.dial SSV @14:51:47.38 Allowing 130s for 25.0ml of remaining 250.0ml @14:51:47.39 Intake.select :open @14:51:49.56 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:51:49.58 SS.reconfigure SSinhale.with maxSpeed: 39 @14:51:49.63 SS.seek 25ml @14:52:35.11 Stroke completed -- waiting for pressure to equalize ... @14:52:38.25 Intake.select :close @14:52:38.41 SSV.dial :exhaust @14:52:39.21 Exhaust.select :open @14:52:39.32 SS.reconfigure SSexhale @14:52:39.37 SS.seek 0ml @14:52:48.41 Exhaust.select :close @14:52:48.53 SSV.dial CBV @14:52:48.56
CBV.dial SSV @14:52:49.37 Allowing 130s for 25.0ml of remaining 225.0ml @14:52:49.38 Intake.select :open @14:52:51.55 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:52:51.57 SS.reconfigure SSinhale.with maxSpeed: 39 @14:52:51.62 SS.seek 25ml @14:53:36.86 Stroke completed -- waiting for pressure to equalize ... @14:53:40.11 Intake.select :close @14:53:40.27 SSV.dial :exhaust @14:53:41.06 Exhaust.select :open @14:53:41.17 SS.reconfigure SSexhale @14:53:41.22 SS.seek 0ml @14:53:50.08 Exhaust.select :close @14:53:50.20 SSV.dial CBV @14:53:50.23
CBV.dial SSV @14:53:51.06 Allowing 130s for 25.0ml of remaining 200.0ml @14:53:51.07 Intake.select :open @14:53:53.24 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:53:53.26 SS.reconfigure SSinhale.with maxSpeed: 39 @14:53:53.31 SS.seek 25ml @14:54:39.11 Stroke completed -- waiting for pressure to equalize ... @14:54:42.36 Intake.select :close @14:54:42.52 SSV.dial :exhaust @14:54:43.31 Exhaust.select :open @14:54:43.43 SS.reconfigure SSexhale @14:54:43.48 SS.seek 0ml @14:54:52.31 Exhaust.select :close @14:54:52.43 SSV.dial CBV @14:54:52.46
CBV.dial SSV @14:54:53.28 Allowing 130s for 25.0ml of remaining 175.0ml @14:54:53.29 Intake.select :open @14:54:55.46 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:54:55.48 SS.reconfigure SSinhale.with maxSpeed: 39 @14:54:55.53 SS.seek 25ml @14:55:25.32 Vacuum too high -- waiting up to 40s @14:55:26.16 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:55:26.18 SS.reconfigure SSinhale.with maxSpeed: 35 @14:55:26.23 SS.seek 25ml @14:55:44.16 Stroke completed -- waiting for pressure to equalize ... @14:55:47.36 Intake.select :close @14:55:47.51 SSV.dial :exhaust @14:55:48.32 Exhaust.select :open @14:55:48.43 SS.reconfigure SSexhale @14:55:48.49 SS.seek 0ml @14:55:57.25 Exhaust.select :close @14:55:57.37 SSV.dial CBV @14:55:57.40
CBV.dial SSV @14:55:58.20 Allowing 130s for 25.0ml of remaining 150.0ml @14:55:58.21 Intake.select :open @14:56:00.91 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:56:00.92 SS.reconfigure SSinhale.with maxSpeed: 35 @14:56:00.97 SS.seek 25ml @14:56:51.73 Stroke completed -- waiting for pressure to equalize ... @14:56:54.98 Intake.select :close @14:56:55.14 SSV.dial :exhaust @14:56:55.95 Exhaust.select :open @14:56:56.06 SS.reconfigure SSexhale @14:56:56.11 SS.seek 0ml @14:57:05.23 Exhaust.select :close @14:57:05.35 SSV.dial CBV @14:57:05.38
CBV.dial SSV @14:57:06.18 Allowing 130s for 25.0ml of remaining 125.0ml @14:57:06.19 Intake.select :open @14:57:08.37 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:57:08.38 SS.reconfigure SSinhale.with maxSpeed: 35 @14:57:08.43 SS.seek 25ml @14:57:58.55 Stroke completed -- waiting for pressure to equalize ... @14:58:01.78 Intake.select :close @14:58:01.94 SSV.dial :exhaust @14:58:02.72 Exhaust.select :open @14:58:02.84 SS.reconfigure SSexhale @14:58:02.89 SS.seek 0ml @14:58:11.75 Exhaust.select :close @14:58:11.87 SSV.dial CBV @14:58:11.90
CBV.dial SSV @14:58:12.73 Allowing 130s for 25.0ml of remaining 100.0ml @14:58:12.74 Intake.select :open @14:58:14.92 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:58:14.93 SS.reconfigure SSinhale.with maxSpeed: 35 @14:58:14.98 SS.seek 25ml @14:59:05.72 Stroke completed -- waiting for pressure to equalize ... @14:59:08.95 Intake.select :close @14:59:09.11 SSV.dial :exhaust @14:59:09.92 Exhaust.select :open @14:59:10.03 SS.reconfigure SSexhale @14:59:10.08 SS.seek 0ml @14:59:19.16 Exhaust.select :close @14:59:19.28 SSV.dial CBV @14:59:19.31
CBV.dial SSV @14:59:20.12 Allowing 130s for 25.0ml of remaining 75.0ml @14:59:20.13 Intake.select :open @14:59:22.30 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:59:22.32 SS.reconfigure SSinhale.with maxSpeed: 35 @14:59:22.37 SS.seek 25ml @15:00:12.80 Stroke completed -- waiting for pressure to equalize ... @15:00:15.99 Intake.select :close @15:00:16.15 SSV.dial :exhaust @15:00:16.96 Exhaust.select :open @15:00:17.07 SS.reconfigure SSexhale @15:00:17.12 SS.seek 0ml @15:00:26.05 Exhaust.select :close @15:00:26.17 SSV.dial CBV @15:00:26.20
CBV.dial SSV @15:00:27.03 Allowing 130s for 25.0ml of remaining 50.0ml @15:00:27.04 Intake.select :open @15:00:29.21 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:00:29.23 SS.reconfigure SSinhale.with maxSpeed: 35 @15:00:29.28 SS.seek 25ml @15:01:20.61 Stroke completed -- waiting for pressure to equalize ... @15:01:23.85 Intake.select :close @15:01:24.01 SSV.dial :exhaust @15:01:24.82 Exhaust.select :open @15:01:24.93 SS.reconfigure SSexhale @15:01:24.98 SS.seek 0ml @15:01:34.16 Exhaust.select :close @15:01:34.28 SSV.dial CBV @15:01:34.31
CBV.dial SSV @15:01:35.12 Allowing 130s for 25.0ml of remaining 25.0ml @15:01:35.13 Intake.select :open @15:01:37.31 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:01:37.32 SS.reconfigure SSinhale.with maxSpeed: 35 @15:01:37.37 SS.seek 24.97ml @15:02:27.61 Stroke completed -- waiting for pressure to equalize ... @15:02:30.85 Intake.select :close @15:02:31.01 SSV.dial :exhaust @15:02:31.82 Exhaust.select :open @15:02:31.93 SS.reconfigure SSexhale @15:02:31.98 SS.seek 0ml @15:02:41.08 Exhaust.select :close @15:02:41.20 Sampled 1500.0ml @15:02:41.22 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @15:02:41.23 Intake.select :close @15:02:41.30 CSV.dial :waste @15:02:41.38 CS.seek 0ml @15:02:41.45 SSV.dial CBV,avoiding: :exhaust @15:02:41.48
CBV.dial SSV @15:02:41.66 CSV.dial :air @15:02:42.43 CS.seek 10ml @15:02:43.43
CTV.dial :air @15:02:44.21 delay 15 seconds @15:02:59.24 SSV.dial :spit,avoiding: :exhaust @15:03:00.60 CTV.dial CSV,avoiding: :intake @15:03:00.65
SS.seek 0ml @15:03:00.78 SSV.dial CBV,avoiding: :exhaust @15:03:06.33 CBV.dial between SSV and waste2 @15:03:06.74 CTV.dial CSV,avoiding: :air @15:03:06.78
CSV.dial CTV @15:03:07.38 CS.reconfigure CSpressurize @15:03:07.43 CS.seek 0ml @15:03:33.26 BubblePoint: Intake @69.4psia, Outlet @13.8psia @15:03:33.27 CBV.dial SSV @15:03:33.63 SS.reconfigure SSconfig @15:03:33.68 SS.seek 10ml @15:03:39.31 delay 15 seconds @15:03:54.45 Evacuated: Intake @34.8psia, Outlet @0.3psia @15:03:54.47 SSV.dial :spit,avoiding: :exhaust @15:03:55.82 SS.reconfigure SSexhale @15:03:55.87 SS.seek 0ml @15:04:00.83 SS.reconfigure SSfast @15:04:00.88 SS.seek 5ml @15:04:03.82 SSV.dial CBV,avoiding: :exhaust @15:04:03.85
CBV.dial SSV @15:04:05.23 CTV.dial :air,avoiding: :intake @15:04:07.10 CSV.dial CTV @15:04:07.15
SS.seek 10ml @15:04:08.14 CSV.dial :air @15:04:08.61 CS.reconfigure CSfast @15:04:08.66 CS.seek 0ml @15:04:09.73 CS.coast @15:04:09.80
SSV.dial :spit,avoiding: :exhaust @15:04:11.18 SS.reconfigure SSexhale @15:04:11.23 SS.seek 0ml @15:04:16.13 SS.reconfigure SSfast @15:04:16.18 SS.seek 5ml @15:04:18.84 SSV.dial CBV,avoiding: :exhaust @15:04:18.87
CBV.dial SSV @15:04:21.27 SSV.dial :spit,avoiding: :exhaust @15:04:22.62 SS.reconfigure SSconfig @15:04:22.67 SS.seek 0ml @15:04:25.83 SS.coast @15:04:25.88 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @15:04:25.89 Intake.select :close @15:04:25.97 CSV.dial :waste @15:04:26.10 SSV.dial CBV,avoiding: :exhaust @15:04:26.13
CBV.dial SSV @15:04:26.77 CS.seek 0ml @15:04:26.85 CSV.dial :air @15:04:27.51
CTV.dial :air @15:04:27.52 delay 15 seconds @15:04:27.62 CS.seek 10ml @15:04:42.55
SSV.dial :spit,avoiding: :exhaust @15:04:43.90 CTV.dial CSV,avoiding: :intake @15:04:43.95
SS.reconfigure SSexhale @15:04:44.05 SS.seek 0ml @15:04:44.26 SSV.dial CBV,avoiding: :exhaust @15:04:51.42 CBV.dial between SSV and waste2 @15:04:51.86 CSV.dial between air and CTV @15:04:52.20 CS.seek 8.235ml @15:04:57.30 CTV.dial CSV,avoiding: :air @15:04:57.33
CSV.dial CTV @15:04:57.65 CS.reconfigure CSpressurize @15:04:57.70 CS.seek 0ml @15:05:19.42 CSV.dial :air @15:05:19.91 CS.reconfigure CSfast @15:05:19.96 CS.seek 10ml @15:05:43.67 CSV.dial between air and CTV @15:05:44.03 CS.seek 3.14ml @15:06:00.71 CTV.dial CSV,avoiding: :air @15:06:00.74
CSV.dial CTV @15:06:01.05 CS.reconfigure CSpressurize @15:06:01.11 CS.seek 0ml @15:06:09.17 BubblePoint: Intake @68.4psia, Outlet @13.2psia @15:06:09.18 CBV.dial SSV @15:06:09.54 SS.reconfigure SSconfig @15:06:09.59 SS.seek 10ml @15:06:14.93 delay 15 seconds @15:06:30.08 Evacuated: Intake @37.5psia, Outlet @15.3psia @15:06:30.09 SSV.dial :spit,avoiding: :exhaust @15:06:31.46 SS.reconfigure SSexhale @15:06:31.51 SS.seek 0ml @15:06:36.68 SS.reconfigure SSfast @15:06:36.73 SS.seek 5ml @15:06:39.94 SSV.dial CBV,avoiding: :exhaust @15:06:39.97
CBV.dial SSV @15:06:41.35 CTV.dial :air,avoiding: :intake @15:06:42.71 CSV.dial CTV @15:06:42.76
SS.seek 10ml @15:06:43.75 CSV.dial :air @15:06:44.24 CS.reconfigure CSfast @15:06:44.29 CS.seek 0ml @15:06:45.38
SSV.dial :spit,avoiding: :exhaust @15:06:45.86 CS.coast @15:06:46.75
SS.reconfigure SSexhale @15:06:46.80 SS.seek 0ml @15:06:51.80 SS.reconfigure SSfast @15:06:51.86 SS.seek 5ml @15:06:54.54 SSV.dial CBV,avoiding: :exhaust @15:06:54.57
CBV.dial SSV @15:06:56.96 SSV.dial :spit,avoiding: :exhaust @15:06:58.32 SS.reconfigure SSconfig @15:06:58.37 SS.seek 0ml @15:07:01.53 SS.coast @15:07:01.58 Lysing: Adding 1.5ml lysis @15:07:01.59 CBV.dial between CSV and waste1,avoiding: :DBV @15:07:03.13 CTV.dial CSV @15:07:04.49 CSV.dial CSR @15:07:05.32 Pulling 1ml of CSR.lysis @15:07:05.33 CSR.select :lysis @15:07:05.46 CS.reconfigure CSconfig @15:07:05.51 CS.seek 1ml @15:07:08.99 delay 5 seconds @15:07:14.01 CSR.select :air @15:07:14.20 CS.seek 6ml @15:07:28.02 delay 2 seconds @15:07:30.03 CSV.dial :waste1 @15:07:31.37 CS.reconfigure CSfast @15:07:31.42 CS.seek :empty @15:07:45.95 CSV.dial CSR @15:07:47.35 Pulling 1.5ml of CSR.lysis @15:07:47.36 CSR.select :lysis @15:07:47.48 CS.reconfigure CSconfig @15:07:47.53 CS.seek 1.5ml @15:07:52.46 delay 5 seconds @15:07:57.48 CSR.select :air @15:07:57.67 CS.seek 4.5ml @15:08:06.30 delay 2 seconds @15:08:08.31 CSV.dial CTV @15:08:08.80 CS.seek 1.275ml @15:08:18.03 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @15:08:18.05 CH.seek 85C,8:00 @15:11:09.15 CH.hold 5:00,85C @15:16:09.32 Collection Puck Heater at 85.1C after lysing @15:16:09.34 CS.seek 0ml @15:16:13.56 CTV.dial between spare and air,avoiding: :intake @15:16:14.63 CSV.dial :air @15:16:15.10 CS.seek 5ml @15:16:28.83 CSV.dial CTV @15:16:29.31 delay 2 seconds @15:16:31.34 CSV.dial :air @15:16:31.81 delay 2 seconds @15:16:33.83 CSV.dial CTV @15:16:34.33 delay 2 seconds @15:16:36.36 CSV.dial :air @15:16:36.84 delay 2 seconds @15:16:38.86 CS.seek 0ml @15:16:52.66 Recovering Lysate into Collection syringe @15:16:52.67 CSV.dial CBV @15:16:53.72 CBV.dial CSV @15:16:54.15 CS.seek 5ml @15:17:07.97 delay 5 seconds @15:17:12.99 CTV.dial :air @15:17:13.54 CS.seek 7ml @15:17:19.72 delay 5 seconds @15:17:24.74 CSV.dial :air2 @15:17:25.33 CS.seek 10ml @15:17:33.91 CSV.dial between air and CTV,avoiding: CBV @15:17:35.23 Lysing Complete @15:17:35.31 Storing sh1 puck in Collection Clamp to tube 1 @15:17:35.50 SC.reconfigure SCtight @15:17:35.55 SC.seek 1 @15:17:37.67 SC.reconfigure SCloose @15:17:37.90 Elbow.reconfigure TightElbow @15:17:37.95 Elbow.seek 1 @15:17:39.87 Forearm.reconfigure TightForearm @15:17:39.92 Forearm.seek 1 + 700 counts @15:17:40.99 SE.reconfigure SEpick @15:17:41.06 SE.seek beamBreak @15:17:42.22 SE.configure SEconfig @15:17:42.40 Elbow.reconfigure LimpElbow @15:17:42.45 Forearm.reconfigure LooseForearm @15:17:42.49 Forearm.seek 1 @15:17:42.81 Elbow.hold @15:17:42.88 Elbow.reconfigure TightElbow @15:17:42.99 SE.seek 20.1 Pucks below Top Plate @15:17:43.54 Forearm.force -20 @15:17:43.58 Forearm.seek 1 + 348 counts @15:17:44.44 Forearm.reconfigure TightForearm @15:17:44.49 Forearm.seek 1 + 700 counts @15:17:44.83 SE.seek 21.1 Pucks below Top Plate @15:17:45.47 Forearm.reconfigure LooseForearm @15:17:45.52 Forearm.seek :retract @15:17:46.42 Elbow.seek CC @15:17:48.13 Elbow.reconfigure LooseElbow @15:17:48.24 Forearm.seek CC @15:17:49.17 Hand.close @15:17:49.82 CC.seek :open @15:17:54.32 Forearm.seek :retract @15:17:55.29 Elbow.reconfigure TightElbow @15:17:55.34 Elbow.seek 1 @15:17:57.20 Elbow.reconfigure LimpElbow @15:17:57.26 Forearm.seek 1 @15:17:58.31 Elbow.hold @15:17:58.44 Elbow.reconfigure TightElbow @15:17:58.51 Hand.open @15:17:59.31 Forearm.force -20 @15:17:59.35 Forearm.seek 1 + 346 counts @15:17:59.68 Forearm.reconfigure TightForearm @15:17:59.73 Forearm.seek 1 + 700 counts @15:18:00.14 SE.seek :down @15:18:00.89 SE.coast @15:18:01.31
Forearm.reconfigure LooseForearm @15:18:01.36 Forearm.seek :retract @15:18:02.29 SC.coast @15:18:02.33 Tranferring lysate to PRV @15:18:02.34 CSV.dial :air @15:18:02.68 CS.seek 10ml @15:18:02.75 CSV.dial between air and CTV @15:18:03.09 AIV.dial :bypass @15:18:03.17 Beginning samplePassC @15:18:03.18 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:18:03.25 Hand.open @15:18:03.70 Elbow.seek FlushPuck::Garage @15:18:05.60 Elbow.reconfigure LooseElbow @15:18:05.71 Forearm.seek :garage @15:18:06.73 Hand.close @15:18:07.44 Forearm.seek :retract @15:18:08.45 Elbow.reconfigure TightElbow @15:18:08.50 Elbow.seek CC @15:18:11.38 Elbow.reconfigure LooseElbow @15:18:11.49 Forearm.reconfigure TightForearm @15:18:11.54 Forearm.seek CC @15:18:13.35 CC.seek :closed @15:18:17.62 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.97 @15:18:17.70 Hand.open @15:18:18.38 Forearm.reconfigure LooseForearm @15:18:18.43 Forearm.seek :retract @15:18:19.39 AWV.select :air @15:18:19.41 AIV.dial :bypass @15:18:19.42 PSV.dial PSR @15:18:20.83 Pulling 1.5ml of PSR.flush @15:18:20.84 PSR.select :flush @15:18:20.97 PS.seek 1.5ml @15:18:37.00 delay 5 seconds @15:18:42.02 PSV.dial :waste1 @15:18:42.52 PS.seek 0ml @15:18:58.49 PSV.dial PSR @15:18:58.96 PSR.select :air @15:18:59.10 PS.seek 2.5ml @15:19:25.28 PRV.dial CBV @15:19:25.31
CBV.dial PRV @15:19:26.17 PSV.dial :mixloop @15:19:27.33 CTV.dial :air,avoiding: :intake @15:19:27.34 PTV.dial :PRVmixing @15:19:27.43 PS.seek 0ml @15:19:53.59 PSV.dial :air @15:19:54.08 PS.seek 2.5ml @15:20:20.18 PSV.dial :mixloop @15:20:20.64 PS.seek 0ml @15:20:46.78 PSV.dial :air @15:20:47.26 PS.seek 2.5ml @15:21:13.37 PSV.dial :mixloop @15:21:13.83 PS.seek 0ml @15:21:40.00 Garaging FlushPuck in Collection Clamp @15:21:40.08 Elbow.reconfigure TightElbow @15:21:40.13 Elbow.seek CC @15:21:40.37 Elbow.reconfigure LooseElbow @15:21:40.48 Forearm.seek CC @15:21:41.47 Hand.close @15:21:42.06 CC.seek :open @15:21:46.42 Forearm.seek :retract @15:21:47.47 Elbow.reconfigure TightElbow @15:21:47.52 Elbow.seek :garage @15:21:50.48 Elbow.reconfigure LooseElbow @15:21:50.59 Forearm.reconfigure TightForearm @15:21:50.64 Forearm.seek :garage @15:21:51.82 Hand.open @15:21:52.51 Forearm.reconfigure LooseForearm @15:21:52.56 Forearm.seek :retract @15:21:53.63 Connecting PS to CS to transfer sample @15:21:53.64 CBV.dial AIV @15:21:53.65 AIV.dial :bypass @15:21:53.66 PSV.dial :mixloop @15:21:53.68 PTV.dial :PRVmixing @15:21:53.69 PRV.dial AIV @15:21:53.70 CTV.dial :air,avoiding: :intake @15:21:54.26 Loading lyfil puck from tube 3 into Collection Clamp @15:21:54.53 SC.reconfigure SCtight @15:21:54.58 SC.seek 3 @15:21:58.32 SC.reconfigure SCloose @15:21:58.55 Elbow.reconfigure TightElbow @15:21:58.60 Elbow.seek 3 @15:22:00.69 Forearm.reconfigure TightForearm @15:22:00.74 Forearm.seek 3 + 700 counts @15:22:01.77 SE.reconfigure SEpick @15:22:01.84 SE.seek beamBreak @15:22:02.82 SE.configure SEconfig @15:22:03.00 Elbow.seek 3 @15:22:03.27 Forearm.seek 3 + 300 counts @15:22:03.53 Forearm.reconfigure LooseForearm @15:22:03.58 Hand.close @15:22:04.21 SE.seek :down @15:22:04.45
Forearm.seek :retract @15:22:05.06 SE.coast @15:22:05.41
Elbow.seek CC @15:22:07.10 Elbow.reconfigure LooseElbow @15:22:07.20 Forearm.reconfigure TightForearm @15:22:07.25 Forearm.seek CC @15:22:08.47 CC.seek :closed @15:22:12.72 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.02 @15:22:12.73 Hand.open @15:22:13.38 Forearm.reconfigure LooseForearm @15:22:13.42 Forearm.seek :retract @15:22:14.42 SC.coast @15:22:14.45 CTV.dial CSV,avoiding: :intake @15:22:15.82 CSV.dial CTV @15:22:16.14 CS.seek 5ml @15:22:29.88 delay 1:20 @15:23:49.91 CS.seek 2.5ml @15:23:57.35 delay 40 seconds @15:24:37.38 CS.seek 0ml @15:24:44.73 delay 1:00 @15:25:44.75 PSV.dial :air @15:25:45.23 PS.seek 2.5ml @15:26:11.37 PSV.dial :mixloop @15:26:11.84 delay 3 seconds @15:26:14.86 PSV.dial :air @15:26:15.34 delay 2 seconds @15:26:17.35 PSV.dial :mixloop @15:26:17.82 delay 3 seconds @15:26:20.83 PSV.dial :waste1 @15:26:22.18 PS.seek :empty @15:26:48.31 CBV.dial :waste1 @15:26:48.80 delay 5 seconds @15:26:53.83 CBV.dial AIV @15:26:54.34 delay 10 seconds @15:27:04.36 CBV.dial :waste1 @15:27:04.87 delay 10 seconds @15:27:14.89 CBV.dial AIV @15:27:15.38 delay 10 seconds @15:27:25.41 CTV.dial CSV,avoiding: :intake @15:27:25.87 CBV.dial between AIV and waste1 @15:27:26.34 CSV.dial :air @15:27:26.83 CS.seek 10ml @15:27:53.34 CSV.dial CTV @15:27:53.82 CS.seek 0ml @15:28:20.69 CBV.dial :waste1 @15:28:21.02 delay 10 seconds @15:28:31.05 CTV.dial :air,avoiding: :intake @15:28:32.47 Storing lyfil puck in Collection Clamp to tube 2 @15:28:32.66 SC.reconfigure SCtight @15:28:32.71 SC.seek 2 @15:28:34.82 SC.reconfigure SCloose @15:28:35.01 SE.reconfigure SEplace @15:28:35.09
Elbow.reconfigure TightElbow @15:28:35.14 Elbow.seek CC @15:28:35.25 SE.seek beamBreak @15:28:35.32
Elbow.reconfigure LooseElbow @15:28:35.42 Forearm.seek CC @15:28:36.43 Hand.close @15:28:37.07 CC.seek :open @15:28:38.04 SE.configure SEconfig @15:28:38.10 SE.seek :place @15:28:41.59
Forearm.seek :retract @15:28:42.57 Elbow.reconfigure TightElbow @15:28:42.62 Elbow.seek 2 @15:28:44.56 Elbow.reconfigure LimpElbow @15:28:44.61 Forearm.seek 2 @15:28:45.64 Elbow.hold @15:28:45.71 Elbow.reconfigure TightElbow @15:28:45.76 Hand.open @15:28:46.59 Forearm.force -20 @15:28:46.63 Forearm.seek 2 + 342 counts @15:28:46.96 Forearm.reconfigure TightForearm @15:28:47.00 Forearm.seek 2 + 700 counts @15:28:47.45 SE.seek :down @15:28:48.61
Forearm.reconfigure LooseForearm @15:28:48.66 Forearm.seek :retract @15:28:50.19 SE.coast @15:28:50.23
SC.coast @15:28:50.27 PSV.dial :air @15:28:51.39 PS.seek 0.499ml @15:28:57.32 delay 3 seconds @15:29:00.33 PSV.dial :mixloop @15:29:00.79 PRV.dial :air @15:29:01.27 delay 3 seconds @15:29:04.29 PRV.dial CBV @15:29:04.77 CBV.dial AIV @15:29:05.26 PRV.dial between CBV and air @15:29:05.60 PRV.dial :air @15:29:05.99 PS.reconfigure PSslow @15:29:06.04 PS.seek 0.599ml @15:29:08.53 PRV.dial :waste2 @15:29:09.27 PS.reconfigure PSconfig @15:29:09.32 PS.seek :empty @15:29:16.76 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:29:16.83 Hand.open @15:29:17.20 Elbow.seek FlushPuck::Garage @15:29:19.16 Elbow.reconfigure LooseElbow @15:29:19.27 Forearm.seek :garage @15:29:20.29 Hand.close @15:29:21.08 Forearm.seek :retract @15:29:22.14 Elbow.reconfigure TightElbow @15:29:22.19 Elbow.seek CC @15:29:25.12 Elbow.reconfigure LooseElbow @15:29:25.24 Forearm.reconfigure TightForearm @15:29:25.29 Forearm.seek CC @15:29:26.57 CC.seek :closed @15:29:30.82 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 1.93 @15:29:30.89 Hand.open @15:29:31.64 Forearm.reconfigure LooseForearm @15:29:31.68 Forearm.seek :retract @15:29:32.75 Rinsing MixLoop with GuSCN @15:29:32.76 PSV.dial :mixloop,avoiding: PBV @15:29:32.77 PTV.dial :PRVmixing,avoiding: :puck @15:29:32.79 PRV.dial :spare1 @15:29:33.91 PS.seek 1.099ml @15:29:45.82 delay 2 seconds @15:29:47.83 PRV.dial :air @15:29:48.87 PS.seek 2.5ml @15:30:03.82 PSV.dial :waste1,avoiding: PBV @15:30:05.15 PS.seek :empty @15:30:31.35 Diluting Lysate in Syringe @15:30:31.36 PSV.dial :PTV,avoiding: PBV @15:30:32.98 PTV.dial :PRV,avoiding: :puck @15:30:33.74 CBV.dial :PRV @15:30:33.75 PRV.dial :air @15:30:33.76 delay 2 seconds @15:30:35.79 PRV.dial CBV @15:30:36.26 delay 2 seconds @15:30:38.28 PS.reconfigure PSslow1 @15:30:38.34 PS.seek 0.1ml @15:30:49.51 delay 5 seconds @15:30:54.53 PRV.dial :spare1 @15:30:55.31 PS.reconfigure PSconfig @15:30:55.36 PS.seek 1ml @15:31:05.35 delay 2 seconds @15:31:07.37 PRV.dial :air @15:31:08.43 PS.seek 2.5ml @15:31:24.42 delay 2 seconds @15:31:26.44 Parking diluted lysate in Mixing Coil @15:31:26.45 PSV.dial :mixloop @15:31:26.93 PTV.dial :PRVmixing,avoiding: :puck @15:31:27.70 PRV.dial :CBV @15:31:28.25 PS.seek 1.5ml @15:31:39.23 delay 2 seconds @15:31:41.25 PSV.dial :waste1,avoiding: PBV @15:31:42.59 PS.seek :empty @15:31:58.67 Beginning samplePassF @15:31:58.75 AWV.select :air @15:31:58.76 AIV.dial :bypass @15:31:58.77 PSV.dial PSR,avoiding: PBV @15:31:59.32 Pulling 1.5ml of PSR.flush @15:31:59.33 PSR.select :flush @15:32:00.00 PS.seek 1.5ml @15:32:15.98 delay 5 seconds @15:32:21.00 PSV.dial :waste1 @15:32:21.50 PS.seek 0ml @15:32:37.53 PSV.dial PSR @15:32:38.14 PSR.select :air @15:32:38.27 PS.seek 2.5ml @15:33:04.42 PRV.dial CBV @15:33:04.45
CBV.dial PRV @15:33:04.47 PSV.dial :PTV,via: :air @15:33:06.48 CTV.dial :air,avoiding: :intake @15:33:06.49 PTV.dial :PRV,avoiding: :puck @15:33:07.26 PS.seek 0ml @15:33:33.35 PSV.dial :air @15:33:34.12 PS.seek 2.5ml @15:34:00.32 PSV.dial :PTV @15:34:01.09 PS.seek 0ml @15:34:27.27 moving diluted lysate into CBV-AIV line @15:34:27.28 PSV.dial :air @15:34:28.03 PS.seek 2.5ml @15:34:54.20 delay 2 seconds @15:34:56.22 PSV.dial :mixloop @15:34:56.68 PTV.dial :PRVmixing,avoiding: :puck @15:34:57.43 PRV.dial CBV @15:34:57.51 PS.seek 1.8ml @15:35:05.42 delay 3 seconds @15:35:08.43 PRV.dial :waste2 @15:35:09.50 PS.seek :empty @15:35:28.51 Clearing PML for modified lysate @15:35:28.52 CTV.dial :air,avoiding: :intake @15:35:28.53 PRV.dial :waste2 @15:35:28.54 PTV.dial :PRV,avoiding: :puck @15:35:29.31 delay 2 seconds @15:35:31.34 PTV.dial :PRVmixing,avoiding: :puck @15:35:32.11 delay 2 seconds @15:35:34.12 PSV.dial :PML @15:35:34.14 delay 2 seconds @15:35:36.16 PRV.dial :air @15:35:36.92 delay 2 seconds @15:35:39.00 PS.seek 1.499ml @15:35:54.98 delay 5 seconds @15:36:00.01 PS.seek 2.5ml @15:36:11.04 PSV.dial :air @15:36:11.52 PS.seek 0ml @15:36:37.73 mixing lysate with speDil @15:36:37.74 PSV.dial :PML @15:36:38.20 CBV.dial :PRV @15:36:38.21 PRV.dial :air @15:36:38.22 delay 2 seconds @15:36:40.24 PRV.dial :speDil @15:36:40.72 delay 2 seconds @15:36:42.82 PS.reconfigure PSslow1 @15:36:42.87 PS.seek 0.225ml @15:37:06.45 delay 5 seconds @15:37:11.47 PRV.dial CBV @15:37:12.26 delay 2 seconds @15:37:14.34 PS.seek 0.475ml @15:37:40.26 delay 5 seconds @15:37:45.29 PRV.dial :air @15:37:45.80 delay 2 seconds @15:37:47.82 PS.seek 0.965ml @15:38:37.86 delay 10 seconds @15:38:47.88 PRV.dial CBV @15:38:48.37 CTV.dial :air,avoiding: :intake @15:38:48.38 PSV.dial :air @15:38:48.84 PS.reconfigure PSconfig @15:38:48.89 PS.seek 2.5ml @15:39:05.27 delay 3 seconds @15:39:08.28 PSV.dial PTV @15:39:09.03 PTV.dial :PRV,avoiding: :puck @15:39:09.80 PS.seek 0ml @15:39:35.95 PSV.dial :air @15:39:36.69 PS.seek 2.5ml @15:40:02.83 delay 3 seconds @15:40:05.84 PSV.dial PTV @15:40:06.59 PTV.dial :PRV,avoiding: :puck @15:40:06.61 PS.seek 0ml @15:40:32.83 Pushing modified lysate into AIV Injection Loop @15:40:32.84 CBV.dial AIV @15:40:32.85 AIV.dial :bypass @15:40:32.86 PRV.dial CBV @15:40:32.88 PSV.dial :air @15:40:34.15 PS.seek 2.5ml @15:41:00.24 delay 3 seconds @15:41:03.25 PSV.dial :PML @15:41:03.72 PTV.dial :PRVmixing,avoiding: :puck @15:41:04.56 PS.reconfigure PSslow1 @15:41:04.61 PS.seek 1.74ml @15:42:22.24 delay 5 seconds @15:42:27.26 AIV.dial :sample @15:42:27.85 AWV.select :waste @15:42:28.02 PS.reconfigure PSslow2 @15:42:28.07 PS.seek 1.22ml @15:44:16.39 delay 15 seconds @15:44:31.41 AIV.dial :bypass @15:44:32.01 PS.reconfigure PSconfig @15:44:32.06 PS.seek 0ml @15:44:45.26 PSV.dial :air @15:44:45.73 PS.seek 2.5ml @15:45:11.85 PSV.dial :PML @15:45:12.33 PS.seek 0ml @15:45:38.45 AIV.dial :coilToMFB @15:45:38.47 AWV.select :air @15:45:38.66 Extracting Nucleic Acid from AIV @15:45:38.70
Beginning samplePassD @15:45:38.74 SPE.hold 2:46:40,55C @15:45:38.82 ASV.dial :SPEdelivery @15:45:38.89
AWV.select :air @15:45:38.90 AIV.dial :bypass @15:45:38.92 PSV.dial PSR @15:45:39.22 delay 20 seconds @15:45:40.06
Pulling 1.5ml of PSR.killMFB @15:45:40.07 PSR.select :killMFB @15:45:40.20 PS.seek 1.5ml @15:45:56.21 delay 5 seconds @15:45:59.25 primeLysateINT @15:45:59.26 AWV.select :air @15:45:59.27 AIV.dial :coilToMFB @15:45:59.28 ATV.dial AIV @15:46:00.94 AS.reconfigure ASslow1 @15:46:00.99 AS.seek 0.299ml @15:46:01.23
PSV.dial :waste1 @15:46:01.73 PS.seek 0ml @15:46:17.79 PSV.dial PSR @15:46:18.40 PSR.select :air @15:46:18.53 PS.seek 2.5ml @15:46:44.68 PRV.dial CBV @15:46:44.71
CBV.dial PRV @15:46:44.72 PSV.dial :mixloop @15:46:45.94 CTV.dial :air,avoiding: :intake @15:46:45.95 PTV.dial :PRVmixing @15:46:45.97 PS.seek 0ml @15:47:01.67 delay 10 seconds @15:47:11.69 ATV.dial :air2 @15:47:12.10
PSV.dial PSR @15:47:12.51 AS.seek 0.329ml @15:47:13.24
Pulling 1.5ml of PSR.flush @15:47:13.25 PSR.select :flush @15:47:13.38 PS.seek 1.5ml @15:47:18.91 ATV.dial :waste2 @15:47:19.47 AS.reconfigure ASnorm @15:47:19.52 AS.seek :empty @15:47:29.37
delay 5 seconds @15:47:34.39 PSV.dial :waste1 @15:47:34.89 PS.seek 0ml @15:47:36.47 Lysate primed! @15:47:36.48 AFV.select :holdingCoil @15:47:36.49 ATV.dial :air2 @15:47:37.10 AS.reconfigure ASslow1 @15:47:37.15 AS.seek 0.069ml @15:47:50.86
PSV.dial PSR @15:47:51.37 PSR.select :air @15:47:51.50 delay 3 seconds @15:47:51.53
PS.seek 2.5ml @15:47:54.52 ATV.dial :AIV @15:47:55.35 AS.seek 0.469ml @15:48:17.65 PRV.dial CBV @15:48:17.68
CBV.dial PRV @15:48:17.69 PSV.dial :mixloop @15:48:19.27 CTV.dial :air,avoiding: :intake @15:48:19.31 PTV.dial :PRVmixing @15:48:19.33 PS.seek 0ml @15:48:45.51 PSV.dial PSR @15:48:46.64 Pulling 1.5ml of PSR.flush @15:48:46.65 PSR.select :flush @15:48:46.78 PS.seek 1.5ml @15:49:02.87 delay 5 seconds @15:49:07.90 PSV.dial :waste1 @15:49:08.39 PS.seek 0ml @15:49:16.06 delay 5 seconds @15:49:21.15 Solid Phase Extraction Heater at 49.1C @15:49:21.16 ATV.dial ASV @15:49:21.55 ASV.dial :SPEdelivery @15:49:21.63 AS.seek 0.041ml @15:49:24.36
PSV.dial PSR @15:49:24.87 PSR.select :air @15:49:25.00 PS.seek 2.5ml @15:49:51.18 PRV.dial CBV @15:49:51.21
CBV.dial PRV @15:49:51.22 PSV.dial :mixloop @15:49:52.27 CTV.dial :air,avoiding: :intake @15:49:52.28 PTV.dial :PRVmixing @15:49:52.30 PS.seek 0ml @15:50:18.42 PSV.dial PSR @15:50:19.55 Pulling 1.5ml of PSR.flush @15:50:19.56 PSR.select :flush @15:50:19.69 PS.seek 1.5ml @15:50:35.68 delay 5 seconds @15:50:40.70 PSV.dial :waste1 @15:50:41.19 PS.seek 0ml @15:50:48.00 delay 10 seconds @15:50:57.17
PSV.dial PSR @15:50:57.79 PSR.select :air @15:50:57.92 PS.seek 2.5ml @15:50:58.02 rinseLysateLineINT @15:50:58.03 AFV.select :carrierSolution @15:50:58.13 delay 2 seconds @15:51:00.16 AS.reconfigure ASconfig @15:51:00.21 AS.seek 1ml @15:51:24.15 PRV.dial CBV @15:51:24.18
CBV.dial PRV @15:51:24.19 PSV.dial :mixloop @15:51:25.27 CTV.dial :air,avoiding: :intake @15:51:25.28 PTV.dial :PRVmixing @15:51:25.30 PS.reconfigure PSslow @15:51:25.35 PS.seek 1.2ml @15:51:52.27 PSV.dial :waste1 @15:51:53.62 PS.reconfigure PSconfig @15:51:53.67 PS.seek 0ml @15:51:55.61 delay 1 second @15:51:56.62 AFV.select :holdingCoil @15:51:56.73 delay 1 second @15:51:57.75 ATV.dial AIV @15:51:58.30 AIV.dial :coilToMFB @15:51:58.31 AWV.select :waste @15:51:58.42 AS.reconfigure ASslow0 @15:51:58.47 AS.seek 0ml @15:52:06.70
PSV.dial :air @15:52:07.77 PS.seek 2.5ml @15:52:33.96 PSV.dial :mixloop @15:52:34.41 PS.reconfigure PSslow @15:52:34.46 PS.seek 0ml @15:53:25.64 PSV.dial :air @15:53:26.13 PS.reconfigure PSconfig @15:53:26.18 PS.seek 2.5ml @15:53:39.35 ATV.dial :air2 @15:53:40.01 AS.reconfigure ASslow1 @15:53:40.06 AS.seek 0.05ml @15:53:50.40 ATV.dial :AIV @15:53:51.21 AS.seek 0.01ml @15:53:52.39
PSV.dial :mixloop @15:53:52.88 PS.reconfigure PSslow @15:53:52.94 PS.seek 0ml @15:53:59.56 ATV.dial :waste @15:54:01.09 AS.seek :empty @15:54:03.53 ethanol wash @15:54:03.54 ATV.dial :air2 @15:54:05.28 AS.reconfigure ASslow2 @15:54:05.33 AS.seek 0.009ml @15:54:15.67 delay 3 seconds @15:54:18.70 Pulling 0.4ml of Ethanol Wash @15:54:18.71 ATV.dial :ethanol @15:54:19.60 AS.reconfigure ASslow1 @15:54:19.65 AS.seek 0.409ml @15:54:44.09
Cleaning Collection Syringe @15:54:44.10 CSV.dial CSR @15:54:44.62 Pulling 4ml of CSR.flush @15:54:44.63 CSR.select :flush @15:54:44.76 CS.seek 4ml @15:54:55.94 delay 5 seconds @15:55:00.96 CSR.select :air @15:55:01.09 CS.seek 10ml @15:55:17.47 CSV.dial CTV @15:55:17.50
CTV.dial CSV @15:55:18.92 CBV.dial :waste1 @15:55:19.42 CS.seek 0ml @15:55:40.34 delay 3 seconds @15:55:43.36 ATV.dial :air1 @15:55:43.77 delay 3 seconds @15:55:45.98
CSV.dial CSR @15:55:46.51 Pulling 4ml of CSR.flush @15:55:46.52 CSR.select :flush @15:55:46.65 CS.seek 4ml @15:55:46.86 AS.seek 0.434ml @15:55:52.27 delay 3 seconds @15:55:55.29 ATV.dial :ethanol @15:55:55.91 AS.seek 0.419ml @15:55:57.86
delay 5 seconds @15:55:59.17 delay 3 seconds @15:56:02.19 ATV.dial ASV @15:56:02.88
CSR.select :air @15:56:03.02 CS.seek 10ml @15:56:03.45 AS.seek 0.092ml @15:56:19.30 CSV.dial CTV @15:56:19.34
CTV.dial CSV @15:56:19.83 CBV.dial :waste1 @15:56:19.85 CS.seek 0ml @15:56:46.48 Garaging FlushPuck in Collection Clamp @15:56:46.55 Elbow.reconfigure TightElbow @15:56:46.60 Elbow.seek CC @15:56:46.86 Elbow.reconfigure LooseElbow @15:56:46.97 Forearm.seek CC @15:56:47.96 Hand.close @15:56:48.61 CC.seek :open @15:56:52.98 Forearm.seek :retract @15:56:53.94 Elbow.reconfigure TightElbow @15:56:53.99 Elbow.seek :garage @15:56:57.11 Elbow.reconfigure LooseElbow @15:56:57.22 Forearm.reconfigure TightForearm @15:56:57.27 Forearm.seek :garage @15:56:58.43 Hand.open @15:56:59.18 Forearm.reconfigure LooseForearm @15:56:59.23 Forearm.seek :retract @15:57:00.25 Whole Cell Archive sampling 1500ml @15:57:00.27 Loading wcr puck from tube 3 into Collection Clamp @15:57:00.53 SC.reconfigure SCtight @15:57:00.59 SC.seek 3 @15:57:02.68 SC.reconfigure SCloose @15:57:02.91 Elbow.reconfigure TightElbow @15:57:02.97 Elbow.seek 3 @15:57:05.57 Forearm.reconfigure TightForearm @15:57:05.63 Forearm.seek 3 + 700 counts @15:57:06.67 SE.reconfigure SEpick @15:57:06.76 SE.seek beamBreak @15:57:07.91 SE.configure SEconfig @15:57:08.09 Elbow.seek 3 @15:57:08.43 Forearm.seek 3 + 300 counts @15:57:08.71 Forearm.reconfigure LooseForearm @15:57:08.76 Hand.close @15:57:09.38 SE.seek :down @15:57:09.57 delay 3 seconds @15:57:09.67 Forearm.seek :retract @15:57:10.29 SE.coast @15:57:10.64 Elbow.seek CC @15:57:12.34 Elbow.reconfigure LooseElbow @15:57:12.46 Forearm.reconfigure TightForearm @15:57:12.51 Forearm.seek CC @15:57:12.59 ATV.dial :waste2 @15:57:13.31 AS.reconfigure ASconfig @15:57:13.36 AS.seek :empty @15:57:13.80 CC.seek :closed @15:57:18.06 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.99 @15:57:18.08 Hand.open @15:57:18.81 Forearm.reconfigure LooseForearm @15:57:18.86 Forearm.seek :retract @15:57:18.99 ATV.dial :air2 @15:57:19.39 AS.reconfigure ASslow1 @15:57:19.44 AS.seek 1ml @15:57:19.85 SC.coast @15:57:19.89 collectSample 1500ml w/bubble point of 55psi @15:57:19.91 Priming 7ml @15:57:19.92 Intake.select :close @15:57:19.93 Exhaust.select :close @15:57:19.95 SS.hold @15:57:20.18 Calibrating pressure sensors @15:57:20.19 CTV.dial :air @15:57:21.55 SSV.dial :spit,avoiding: :exhaust @15:57:21.57 SS.reconfigure SSfast @15:57:21.62 SS.seek 5ml @15:57:24.20 SSV.dial CBV @15:57:24.23 CBV.dial SSV @15:57:25.68 delay 3.8 seconds @15:57:29.62 SSV.dial :spit @15:57:30.99 SS.seek 0ml @15:57:33.58 SSV.dial CBV @15:57:34.96 CTV.dial :intake @15:57:35.75 SSV.dial CBV @15:57:35.78 CBV.dial SSV @15:57:35.79 Allowing 40s for 7.0ml of remaining 7.0ml @15:57:35.81 Intake.select :open @15:57:38.50 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:57:38.52 SS.reconfigure SSinhale.with maxSpeed: 150 @15:57:38.57 SS.seek 7ml @15:57:42.22 Vacuum too high -- waiting up to 40s @15:57:44.38 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:57:44.40 SS.reconfigure SSinhale.with maxSpeed: 135 @15:57:44.45 SS.seek 7ml @15:57:47.20 Stroke completed -- waiting for pressure to equalize ... @15:57:51.71 Intake.select :close @15:57:51.88 SSV.dial :exhaust @15:57:52.71 Exhaust.select :open @15:57:52.82 SS.reconfigure SSexhale @15:57:52.87 SS.seek 0ml @15:57:56.56 Exhaust.select :close @15:57:56.68 Sampling 1500.0ml @15:57:56.70 Intake.select :close @15:57:56.71 Exhaust.select :close @15:57:56.72 SS.reconfigure SSconfig @15:57:56.77 SS.hold @15:57:56.99 CTV.dial :intake @15:57:57.01 SSV.dial CBV @15:57:57.04 CBV.dial SSV @15:57:57.85 Allowing 130s for 25.0ml of remaining 1500.0ml @15:57:57.86 Intake.select :open @15:58:00.04 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:58:00.06 SS.reconfigure SSinhale.with maxSpeed: 135 @15:58:00.11 SS.seek 25ml @15:58:03.88 Vacuum too high -- waiting up to 40s @15:58:05.50 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:58:05.52 SS.reconfigure SSinhale.with maxSpeed: 122 @15:58:05.57 SS.seek 25ml @15:58:08.32 Vacuum too high -- waiting up to 40s @15:58:09.97 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:58:09.99 SS.reconfigure SSinhale.with maxSpeed: 110 @15:58:10.04 SS.seek 25ml @15:58:12.85 Vacuum too high -- waiting up to 40s @15:58:14.23 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:58:14.82 SS.reconfigure SSinhale.with maxSpeed: 99 @15:58:14.87 SS.seek 25ml @15:58:18.38 Vacuum too high -- waiting up to 40s @15:58:19.77 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:58:19.79 SS.reconfigure SSinhale.with maxSpeed: 89 @15:58:19.84 SS.seek 25ml @15:58:23.07 Vacuum too high -- waiting up to 40s @15:58:24.44 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:58:24.46 SS.reconfigure SSinhale.with maxSpeed: 80 @15:58:24.51 SS.seek 25ml @15:58:28.07 Vacuum too high -- waiting up to 40s @15:58:29.46 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:58:29.48 SS.reconfigure SSinhale.with maxSpeed: 72 @15:58:29.54 SS.seek 25ml @15:58:34.15 Vacuum too high -- waiting up to 40s @15:58:35.26 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:58:35.28 SS.reconfigure SSinhale.with maxSpeed: 65 @15:58:35.33 SS.seek 25ml @15:58:36.41 Stroke completed -- waiting for pressure to equalize ... @15:58:38.40 Intake.select :close @15:58:38.57 SSV.dial :exhaust @15:58:39.38 Exhaust.select :open @15:58:39.50 SS.reconfigure SSexhale @15:58:39.56 SS.seek 0ml @15:58:48.32 Exhaust.select :close @15:58:48.43 SSV.dial CBV @15:58:48.46 CBV.dial SSV @15:58:49.29 Allowing 130s for 25.0ml of remaining 1475.0ml @15:58:49.30 Intake.select :open @15:58:51.49 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:58:51.51 SS.reconfigure SSinhale.with maxSpeed: 65 @15:58:51.55 SS.seek 25ml @15:58:59.44 Vacuum too high -- waiting up to 40s @15:59:00.56 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:59:00.59 SS.reconfigure SSinhale.with maxSpeed: 59 @15:59:00.63 SS.seek 25ml @15:59:14.60 Vacuum too high -- waiting up to 40s @15:59:15.70 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:59:15.73 SS.reconfigure SSinhale.with maxSpeed: 53 @15:59:15.77 SS.seek 25ml @15:59:26.86 Stroke completed -- waiting for pressure to equalize ... @15:59:29.48 Intake.select :close @15:59:29.64 SSV.dial :exhaust @15:59:30.43 Exhaust.select :open @15:59:30.54 SS.reconfigure SSexhale @15:59:30.60 SS.seek 0ml @15:59:39.53 Exhaust.select :close @15:59:39.65 SSV.dial CBV @15:59:39.68 CBV.dial SSV @15:59:40.48 Allowing 130s for 25.0ml of remaining 1450.0ml @15:59:40.49 Intake.select :open @15:59:42.67 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:59:42.69 SS.reconfigure SSinhale.with maxSpeed: 53 @15:59:42.74 SS.seek 25ml @16:00:16.59 Stroke completed -- waiting for pressure to equalize ... @16:00:19.20 Intake.select :close @16:00:19.36 SSV.dial :exhaust @16:00:20.15 Exhaust.select :open @16:00:20.26 SS.reconfigure SSexhale @16:00:20.32 SS.seek 0ml @16:00:29.39 Exhaust.select :close @16:00:29.51 SSV.dial CBV @16:00:29.54 CBV.dial SSV @16:00:30.37 Allowing 130s for 25.0ml of remaining 1425.0ml @16:00:30.38 Intake.select :open @16:00:32.57 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:00:32.59 SS.reconfigure SSinhale.with maxSpeed: 53 @16:00:32.64 SS.seek 25ml @16:00:41.05 delay 5 seconds @16:00:46.07 ATV.dial ASV @16:00:47.02 ASV.dial :SPEdelivery @16:00:47.04 AS.seek 0.02ml @16:01:06.12 Stroke completed -- waiting for pressure to equalize ... @16:01:08.73 Intake.select :close @16:01:08.90 SSV.dial :exhaust @16:01:09.71 Exhaust.select :open @16:01:09.82 SS.reconfigure SSexhale @16:01:09.87 SS.seek 0ml @16:01:18.81 Exhaust.select :close @16:01:18.93 SSV.dial CBV @16:01:18.96 CBV.dial SSV @16:01:19.77 Allowing 130s for 25.0ml of remaining 1400.0ml @16:01:19.78 Intake.select :open @16:01:21.97 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:01:21.99 SS.reconfigure SSinhale.with maxSpeed: 53 @16:01:22.04 SS.seek 25ml @16:01:55.87 Stroke completed -- waiting for pressure to equalize ... @16:01:58.47 Intake.select :close @16:01:58.64 SSV.dial :exhaust @16:01:59.43 Exhaust.select :open @16:01:59.54 SS.reconfigure SSexhale @16:01:59.59 SS.seek 0ml @16:02:08.34 Exhaust.select :close @16:02:08.46 SSV.dial CBV @16:02:08.49 CBV.dial SSV @16:02:09.31 Allowing 130s for 25.0ml of remaining 1375.0ml @16:02:09.32 Intake.select :open @16:02:11.51 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:02:11.53 SS.reconfigure SSinhale.with maxSpeed: 53 @16:02:12.13 SS.seek 25ml @16:02:46.39 Stroke completed -- waiting for pressure to equalize ... @16:02:49.01 Intake.select :close @16:02:49.17 SSV.dial :exhaust @16:02:49.96 Exhaust.select :open @16:02:50.08 SS.reconfigure SSexhale @16:02:50.13 SS.seek 0ml @16:02:58.98 Exhaust.select :close @16:02:59.09 SSV.dial CBV @16:02:59.12 CBV.dial SSV @16:02:59.95 Allowing 130s for 25.0ml of remaining 1350.0ml @16:02:59.96 Intake.select :open @16:03:02.14 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:03:02.16 SS.reconfigure SSinhale.with maxSpeed: 53 @16:03:02.21 SS.seek 25ml @16:03:36.51 Stroke completed -- waiting for pressure to equalize ... @16:03:39.12 Intake.select :close @16:03:39.28 SSV.dial :exhaust @16:03:40.07 Exhaust.select :open @16:03:40.18 SS.reconfigure SSexhale @16:03:40.24 SS.seek 0ml @16:03:49.25 Exhaust.select :close @16:03:49.37 SSV.dial CBV @16:03:49.40 CBV.dial SSV @16:03:50.21 Allowing 130s for 25.0ml of remaining 1325.0ml @16:03:50.22 Intake.select :open @16:03:52.41 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:03:52.43 SS.reconfigure SSinhale.with maxSpeed: 53 @16:03:52.48 SS.seek 25ml @16:04:04.38 delay 5 seconds @16:04:09.40 ATV.dial :air2 @16:04:10.39 AS.seek 0.52ml @16:04:26.25 Stroke completed -- waiting for pressure to equalize ... @16:04:28.86 Intake.select :close @16:04:29.03 SSV.dial :exhaust @16:04:29.82 Exhaust.select :open @16:04:29.93 SS.reconfigure SSexhale @16:04:29.99 SS.seek 0ml @16:04:39.22 Exhaust.select :close @16:04:39.34 SSV.dial CBV @16:04:39.37 CBV.dial SSV @16:04:40.20 Allowing 130s for 25.0ml of remaining 1300.0ml @16:04:40.21 Intake.select :open @16:04:42.40 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:04:42.42 SS.reconfigure SSinhale.with maxSpeed: 53 @16:04:42.46 SS.seek 25ml @16:05:16.41 Stroke completed -- waiting for pressure to equalize ... @16:05:19.66 Intake.select :close @16:05:19.82 SSV.dial :exhaust @16:05:20.62 Exhaust.select :open @16:05:20.73 SS.reconfigure SSexhale @16:05:20.78 SS.seek 0ml @16:05:29.91 Exhaust.select :close @16:05:30.03 SSV.dial CBV @16:05:30.06 CBV.dial SSV @16:05:30.87 Allowing 130s for 25.0ml of remaining 1275.0ml @16:05:30.88 Intake.select :open @16:05:33.62 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:05:33.64 SS.reconfigure SSinhale.with maxSpeed: 53 @16:05:33.69 SS.seek 25ml @16:05:51.25 delay 5 seconds @16:05:56.27 ATV.dial ASV @16:05:57.22 ASV.dial :wasteR @16:05:57.59 AS.seek 0.3ml @16:06:07.28 Stroke completed -- waiting for pressure to equalize ... @16:06:10.52 Intake.select :close @16:06:10.68 SSV.dial :exhaust @16:06:11.48 Exhaust.select :open @16:06:11.59 SS.reconfigure SSexhale @16:06:11.65 SS.seek 0ml @16:06:20.82 Exhaust.select :close @16:06:20.93 SSV.dial CBV @16:06:20.96 CBV.dial SSV @16:06:21.79 Allowing 130s for 25.0ml of remaining 1250.0ml @16:06:21.80 Intake.select :open @16:06:23.99 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:06:24.01 SS.reconfigure SSinhale.with maxSpeed: 53 @16:06:24.06 SS.seek 25ml @16:06:42.11 delay 20 seconds @16:06:57.61 Stroke completed -- waiting for pressure to equalize ... @16:07:00.93 Intake.select :close @16:07:01.09 SSV.dial :exhaust @16:07:01.90 Exhaust.select :open @16:07:02.01 SS.reconfigure SSexhale @16:07:02.07 SS.seek 0ml @16:07:02.13 AS.seek 0.07ml @16:07:11.28 Exhaust.select :close @16:07:11.40 SSV.dial CBV @16:07:11.43 CBV.dial SSV @16:07:12.26 Allowing 130s for 25.0ml of remaining 1225.0ml @16:07:12.27 Intake.select :open @16:07:14.46 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:07:14.48 SS.reconfigure SSinhale.with maxSpeed: 53 @16:07:14.53 SS.seek 25ml @16:07:48.59 Stroke completed -- waiting for pressure to equalize ... @16:07:48.64 delay 20 seconds @16:07:51.84 Intake.select :close @16:07:52.01 SSV.dial :exhaust @16:07:52.81 Exhaust.select :open @16:07:52.92 SS.reconfigure SSexhale @16:07:52.97 SS.seek 0ml @16:08:01.73 Exhaust.select :close @16:08:01.85 SSV.dial CBV @16:08:01.88 CBV.dial SSV @16:08:02.70 Allowing 130s for 25.0ml of remaining 1200.0ml @16:08:02.71 Intake.select :open @16:08:04.90 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:08:04.92 SS.reconfigure SSinhale.with maxSpeed: 53 @16:08:04.97 SS.seek 25ml @16:08:08.66 ATV.dial :waste2 @16:08:09.38 AS.reconfigure ASconfig @16:08:09.43 AS.seek :empty @16:08:13.83 Elution @16:08:13.84 ATV.dial :air1 @16:08:14.90 AS.reconfigure ASslow1 @16:08:14.95 AS.seek 0.049ml @16:08:25.29 delay 3 seconds @16:08:28.31 ATV.dial :ElutionWater @16:08:29.53 AS.seek 0.109ml @16:08:35.19 Vacuum too high -- waiting up to 40s @16:08:36.30 Intake @33.6psia, vacuum from 20.0 to 24.0psi @16:08:36.32 SS.reconfigure SSinhale.with maxSpeed: 48 @16:08:36.37 SS.seek 25ml @16:08:40.91 Stroke completed -- waiting for pressure to equalize ... @16:08:41.93 delay 3 seconds @16:08:43.50 Intake.select :close @16:08:43.66 SSV.dial :exhaust @16:08:44.46 Exhaust.select :open @16:08:44.57 SS.reconfigure SSexhale @16:08:44.63 SS.seek 0ml @16:08:44.96 ATV.dial ASV,via: :air2 @16:08:46.88 ASV.dial :SPEelution @16:08:47.44 AFV.select :carrierSolution @16:08:47.60 AS.reconfigure ASnorm @16:08:47.66 AS.seek 0.759ml @16:08:53.77 Exhaust.select :close @16:08:53.89 SSV.dial CBV @16:08:53.92 CBV.dial SSV @16:08:54.74 Allowing 130s for 25.0ml of remaining 1175.0ml @16:08:54.75 Intake.select :open @16:08:56.93 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:08:56.95 SS.reconfigure SSinhale.with maxSpeed: 48 @16:08:57.00 SS.seek 25ml @16:09:20.77 delay 3 seconds @16:09:23.79 AFV.select :holdingCoil @16:09:23.95 AS.reconfigure ASslow1 @16:09:24.00 AS.seek 0.474ml @16:09:34.28 Stroke completed -- waiting for pressure to equalize ... @16:09:36.91 Intake.select :close @16:09:37.07 SSV.dial :exhaust @16:09:37.88 Exhaust.select :open @16:09:37.99 SS.reconfigure SSexhale @16:09:38.04 SS.seek 0ml @16:09:47.11 Exhaust.select :close @16:09:47.23 SSV.dial CBV @16:09:47.26 CBV.dial SSV @16:09:48.09 Allowing 130s for 25.0ml of remaining 1150.0ml @16:09:48.10 Intake.select :open @16:09:50.28 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:09:50.30 SS.reconfigure SSinhale.with maxSpeed: 48 @16:09:50.35 SS.seek 25ml @16:10:21.65 delay 5 seconds @16:10:26.75 AS.reconfigure ASslow2 @16:10:26.80 AS.seek 0.439ml @16:10:27.48 Stroke completed -- waiting for pressure to equalize ... @16:10:30.09 Intake.select :close @16:10:30.25 SSV.dial :exhaust @16:10:31.04 Exhaust.select :open @16:10:31.15 SS.reconfigure SSexhale @16:10:31.20 SS.seek 0ml @16:10:39.95 Exhaust.select :close @16:10:40.06 SSV.dial CBV @16:10:40.09 CBV.dial SSV @16:10:40.92 Allowing 130s for 25.0ml of remaining 1125.0ml @16:10:40.93 Intake.select :open @16:10:43.12 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:10:43.14 SS.reconfigure SSinhale.with maxSpeed: 48 @16:10:43.19 SS.seek 25ml @16:11:02.18 delay 1:00 @16:11:21.25 Stroke completed -- waiting for pressure to equalize ... @16:11:24.53 Intake.select :close @16:11:24.70 SSV.dial :exhaust @16:11:25.49 Exhaust.select :open @16:11:25.61 SS.reconfigure SSexhale @16:11:25.67 SS.seek 0ml @16:11:34.66 Exhaust.select :close @16:11:34.78 SSV.dial CBV @16:11:34.81 CBV.dial SSV @16:11:35.63 Allowing 130s for 25.0ml of remaining 1100.0ml @16:11:35.64 Intake.select :open @16:11:37.83 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:11:37.85 SS.reconfigure SSinhale.with maxSpeed: 48 @16:11:37.90 SS.seek 25ml @16:12:02.21 pushing eluate to holding coil @16:12:02.28 AS.seek 0.279ml @16:12:14.91 Stroke completed -- waiting for pressure to equalize ... @16:12:18.16 Intake.select :close @16:12:18.33 SSV.dial :exhaust @16:12:19.13 Exhaust.select :open @16:12:19.24 SS.reconfigure SSexhale @16:12:19.29 SS.seek 0ml @16:12:27.97 Exhaust.select :close @16:12:28.09 SSV.dial CBV @16:12:28.12 CBV.dial SSV @16:12:28.95 Allowing 130s for 25.0ml of remaining 1075.0ml @16:12:28.96 Intake.select :open @16:12:31.15 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:12:31.17 SS.reconfigure SSinhale.with maxSpeed: 48 @16:12:31.22 SS.seek 25ml @16:13:08.72 Stroke completed -- waiting for pressure to equalize ... @16:13:11.96 Intake.select :close @16:13:12.12 SSV.dial :exhaust @16:13:12.92 Exhaust.select :open @16:13:13.03 SS.reconfigure SSexhale @16:13:13.09 SS.seek 0ml @16:13:21.83 Exhaust.select :close @16:13:21.95 SSV.dial CBV @16:13:21.98 CBV.dial SSV @16:13:22.81 Allowing 130s for 25.0ml of remaining 1050.0ml @16:13:22.82 Intake.select :open @16:13:25.00 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:13:25.02 SS.reconfigure SSinhale.with maxSpeed: 48 @16:13:25.07 SS.seek 25ml @16:14:02.53 Stroke completed -- waiting for pressure to equalize ... @16:14:05.77 Intake.select :close @16:14:05.94 SSV.dial :exhaust @16:14:06.74 Exhaust.select :open @16:14:06.85 SS.reconfigure SSexhale @16:14:06.90 SS.seek 0ml @16:14:15.97 Exhaust.select :close @16:14:16.66 SSV.dial CBV @16:14:16.69 CBV.dial SSV @16:14:17.51 Allowing 130s for 25.0ml of remaining 1025.0ml @16:14:17.52 Intake.select :open @16:14:19.70 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:14:19.72 SS.reconfigure SSinhale.with maxSpeed: 48 @16:14:19.77 SS.seek 25ml @16:14:43.44 delay 30 seconds @16:14:56.86 Stroke completed -- waiting for pressure to equalize ... @16:15:00.10 Intake.select :close @16:15:00.26 SSV.dial :exhaust @16:15:01.06 Exhaust.select :open @16:15:01.17 SS.reconfigure SSexhale @16:15:01.23 SS.seek 0ml @16:15:10.51 Exhaust.select :close @16:15:10.63 SSV.dial CBV @16:15:10.66 CBV.dial SSV @16:15:11.48 Allowing 130s for 25.0ml of remaining 1000.0ml @16:15:11.49 Intake.select :open @16:15:13.47 ATV.dial :waste,via: :air3 @16:15:13.68 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:15:13.70 SS.reconfigure SSinhale.with maxSpeed: 48 @16:15:13.75 SS.seek 25ml @16:15:15.05 AS.reconfigure ASconfig @16:15:15.10 AS.seek :empty @16:15:31.47 Parking Bleach in LysateSource line and SPEcolumn @16:15:31.48 ATV.dial :air3 @16:15:32.28 AS.reconfigure ASslow1 @16:15:32.33 AS.seek 0.029ml @16:15:38.65 ATV.dial :ElutionWater @16:15:39.25 AS.seek 0.059ml @16:15:45.53 ATV.dial :air3 @16:15:46.14 AS.seek 0.069ml @16:15:48.43 delay 2 seconds @16:15:50.44 ATV.dial :bleach @16:15:50.71 Stroke completed -- waiting for pressure to equalize ... @16:15:50.90 AS.seek 0.769ml @16:15:53.96 Intake.select :close @16:15:54.12 SSV.dial :exhaust @16:15:54.93 Exhaust.select :open @16:15:55.04 SS.reconfigure SSexhale @16:15:55.09 SS.seek 0ml @16:16:04.20 Exhaust.select :close @16:16:04.32 SSV.dial CBV @16:16:04.35 CBV.dial SSV @16:16:05.17 Allowing 130s for 25.0ml of remaining 975.0ml @16:16:05.18 Intake.select :open @16:16:07.36 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:16:07.38 SS.reconfigure SSinhale.with maxSpeed: 48 @16:16:07.43 SS.seek 25ml @16:16:44.59 Stroke completed -- waiting for pressure to equalize ... @16:16:47.84 Intake.select :close @16:16:48.00 SSV.dial :exhaust @16:16:48.79 Exhaust.select :open @16:16:48.90 SS.reconfigure SSexhale @16:16:48.95 SS.seek 0ml @16:16:58.12 Exhaust.select :close @16:16:58.24 SSV.dial CBV @16:16:58.27 CBV.dial SSV @16:16:59.67 Allowing 130s for 25.0ml of remaining 950.0ml @16:16:59.68 Intake.select :open @16:17:01.87 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:17:01.89 SS.reconfigure SSinhale.with maxSpeed: 48 @16:17:01.94 SS.seek 25ml @16:17:38.83 Stroke completed -- waiting for pressure to equalize ... @16:17:42.09 Intake.select :close @16:17:42.26 SSV.dial :exhaust @16:17:43.06 Exhaust.select :open @16:17:43.17 SS.reconfigure SSexhale @16:17:43.23 SS.seek 0ml @16:17:52.38 Exhaust.select :close @16:17:52.49 SSV.dial CBV @16:17:52.52 CBV.dial SSV @16:17:53.32 Allowing 130s for 25.0ml of remaining 925.0ml @16:17:53.33 Intake.select :open @16:17:55.52 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:17:55.54 SS.reconfigure SSinhale.with maxSpeed: 48 @16:17:55.59 SS.seek 25ml @16:18:11.94 delay 3 seconds @16:18:14.96 ATV.dial :air3 @16:18:15.49 delay 3 seconds @16:18:18.58 AS.seek 0.794ml @16:18:23.88 delay 3 seconds @16:18:26.90 ATV.dial :bleach @16:18:27.50 AS.seek 0.779ml @16:18:30.77 delay 3 seconds @16:18:32.51 Stroke completed -- waiting for pressure to equalize ... @16:18:33.81 ATV.dial :AIV @16:18:35.00 AS.reconfigure ASconfig @16:18:35.10 AS.seek 0.015ml @16:18:36.58 Intake.select :close @16:18:36.74 SSV.dial :exhaust @16:18:37.54 Exhaust.select :open @16:18:37.65 SS.reconfigure SSexhale @16:18:37.70 SS.seek 0ml @16:18:46.57 Exhaust.select :close @16:18:46.68 SSV.dial CBV @16:18:46.71 CBV.dial SSV @16:18:47.52 Allowing 130s for 25.0ml of remaining 900.0ml @16:18:47.53 Intake.select :open @16:18:49.72 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:18:49.74 SS.reconfigure SSinhale.with maxSpeed: 48 @16:18:49.80 SS.seek 25ml @16:19:19.40 ATV.dial :waste @16:19:20.79 AS.seek :empty @16:19:21.98 ATV.dial :air3 @16:19:22.62 AS.reconfigure ASslow1 @16:19:22.67 AS.seek 0.009ml @16:19:25.09 delay 2 seconds @16:19:27.11 ATV.dial :bleach @16:19:27.36 Stroke completed -- waiting for pressure to equalize ... @16:19:27.58 AS.seek 0.579ml @16:19:31.25 Intake.select :close @16:19:31.41 SSV.dial :exhaust @16:19:32.67 Exhaust.select :open @16:19:32.88 SS.reconfigure SSexhale @16:19:32.94 SS.seek 0ml @16:19:42.20 Exhaust.select :close @16:19:42.32 SSV.dial CBV @16:19:42.35 CBV.dial SSV @16:19:43.17 Allowing 130s for 25.0ml of remaining 875.0ml @16:19:43.18 Intake.select :open @16:19:45.36 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:19:45.38 SS.reconfigure SSinhale.with maxSpeed: 48 @16:19:45.43 SS.seek 25ml @16:20:13.19 Vacuum too high -- waiting up to 40s @16:20:14.31 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:20:14.33 SS.reconfigure SSinhale.with maxSpeed: 43 @16:20:14.39 SS.seek 25ml @16:20:24.92 Stroke completed -- waiting for pressure to equalize ... @16:20:28.15 Intake.select :close @16:20:28.31 SSV.dial :exhaust @16:20:29.10 Exhaust.select :open @16:20:29.21 SS.reconfigure SSexhale @16:20:29.27 SS.seek 0ml @16:20:38.08 Exhaust.select :close @16:20:38.20 SSV.dial CBV @16:20:38.23 CBV.dial SSV @16:20:39.04 Allowing 130s for 25.0ml of remaining 850.0ml @16:20:39.05 Intake.select :open @16:20:41.23 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:20:41.25 SS.reconfigure SSinhale.with maxSpeed: 43 @16:20:41.30 SS.seek 25ml @16:21:22.54 delay 3 seconds @16:21:22.89 Stroke completed -- waiting for pressure to equalize ... @16:21:25.55 ATV.dial :air3 @16:21:26.12 delay 3 seconds @16:21:26.21 Intake.select :close @16:21:26.37 SSV.dial :exhaust @16:21:27.17 Exhaust.select :open @16:21:27.28 SS.reconfigure SSexhale @16:21:27.33 SS.seek 0ml @16:21:29.23 AS.seek 0.604ml @16:21:34.60 delay 3 seconds @16:21:36.44 Exhaust.select :close @16:21:36.56 SSV.dial CBV @16:21:36.59 CBV.dial SSV @16:21:37.42 Allowing 130s for 25.0ml of remaining 825.0ml @16:21:37.43 Intake.select :open @16:21:37.61 ATV.dial :bleach @16:21:38.22 AS.seek 0.589ml @16:21:39.62 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:21:39.64 SS.reconfigure SSinhale.with maxSpeed: 43 @16:21:39.69 SS.seek 25ml @16:21:41.48 delay 3 seconds @16:21:44.49 ATV.dial :ASV @16:21:45.46 ASV.dial :wasteR @16:21:46.03 AS.seek 0ml @16:22:20.78 Stroke completed -- waiting for pressure to equalize ... @16:22:24.04 Intake.select :close @16:22:24.78 SSV.dial :exhaust @16:22:25.59 Exhaust.select :open @16:22:25.70 SS.reconfigure SSexhale @16:22:25.76 SS.seek 0ml @16:22:34.54 Exhaust.select :close @16:22:34.65 SSV.dial CBV @16:22:34.68 CBV.dial SSV @16:22:35.52 Allowing 130s for 25.0ml of remaining 800.0ml @16:22:35.53 Intake.select :open @16:22:37.72 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:22:37.74 SS.reconfigure SSinhale.with maxSpeed: 43 @16:22:37.79 SS.seek 25ml @16:23:19.42 Stroke completed -- waiting for pressure to equalize ... @16:23:22.65 Intake.select :close @16:23:22.81 SSV.dial :exhaust @16:23:23.62 Exhaust.select :open @16:23:23.73 SS.reconfigure SSexhale @16:23:23.79 SS.seek 0ml @16:23:32.59 Exhaust.select :close @16:23:32.71 SSV.dial CBV @16:23:32.74 CBV.dial SSV @16:23:33.57 Allowing 130s for 25.0ml of remaining 775.0ml @16:23:33.58 Intake.select :open @16:23:35.77 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:23:35.79 SS.reconfigure SSinhale.with maxSpeed: 43 @16:23:35.84 SS.seek 25ml @16:23:44.79 delay 1:00 @16:24:17.53 Stroke completed -- waiting for pressure to equalize ... @16:24:20.76 Intake.select :close @16:24:20.93 SSV.dial :exhaust @16:24:21.74 Exhaust.select :open @16:24:21.85 SS.reconfigure SSexhale @16:24:21.90 SS.seek 0ml @16:24:31.11 Exhaust.select :close @16:24:31.23 SSV.dial CBV @16:24:31.26 CBV.dial SSV @16:24:32.09 Allowing 130s for 25.0ml of remaining 750.0ml @16:24:32.10 Intake.select :open @16:24:34.28 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:24:34.30 SS.reconfigure SSinhale.with maxSpeed: 43 @16:24:34.35 SS.seek 25ml @16:24:44.81 AFV.select :carrierSolution @16:24:44.92 delay 2 seconds @16:24:46.95 AS.reconfigure ASconfig @16:24:47.00 AS.seek 1ml @16:25:15.47 Stroke completed -- waiting for pressure to equalize ... @16:25:19.35 Intake.select :close @16:25:19.52 SSV.dial :exhaust @16:25:20.31 Exhaust.select :open @16:25:20.42 SS.reconfigure SSexhale @16:25:20.47 SS.seek 0ml @16:25:29.56 Exhaust.select :close @16:25:29.68 SSV.dial CBV @16:25:29.71 CBV.dial SSV @16:25:30.54 Allowing 130s for 25.0ml of remaining 725.0ml @16:25:30.55 Intake.select :open @16:25:32.74 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:25:32.76 SS.reconfigure SSinhale.with maxSpeed: 43 @16:25:32.81 SS.seek 25ml @16:25:44.76 delay 1 second @16:25:45.77 AFV.select :holdingCoil @16:25:46.35 delay 1 second @16:25:47.46 ATV.dial ASV @16:25:47.48 ASV.dial :wasteR @16:25:47.50 AS.reconfigure ASslow1 @16:25:47.54 AS.seek 0ml @16:26:14.14 Stroke completed -- waiting for pressure to equalize ... @16:26:17.38 Intake.select :close @16:26:17.55 SSV.dial :exhaust @16:26:18.35 Exhaust.select :open @16:26:18.46 SS.reconfigure SSexhale @16:26:18.52 SS.seek 0ml @16:26:27.88 Exhaust.select :close @16:26:27.99 SSV.dial CBV @16:26:28.02 CBV.dial SSV @16:26:28.84 Allowing 130s for 25.0ml of remaining 700.0ml @16:26:28.85 Intake.select :open @16:26:31.04 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:26:31.06 SS.reconfigure SSinhale.with maxSpeed: 43 @16:26:31.11 SS.seek 25ml @16:27:12.53 Stroke completed -- waiting for pressure to equalize ... @16:27:16.44 Intake.select :close @16:27:16.60 SSV.dial :exhaust @16:27:17.42 Exhaust.select :open @16:27:17.53 SS.reconfigure SSexhale @16:27:17.60 SS.seek 0ml @16:27:26.42 Exhaust.select :close @16:27:26.54 SSV.dial CBV @16:27:26.57 CBV.dial SSV @16:27:27.42 Allowing 130s for 25.0ml of remaining 675.0ml @16:27:27.43 Intake.select :open @16:27:29.62 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:27:29.64 SS.reconfigure SSinhale.with maxSpeed: 43 @16:27:29.69 SS.seek 25ml @16:28:11.30 Stroke completed -- waiting for pressure to equalize ... @16:28:15.18 Intake.select :close @16:28:15.34 SSV.dial :exhaust @16:28:16.15 Exhaust.select :open @16:28:16.26 SS.reconfigure SSexhale @16:28:16.33 SS.seek 0ml @16:28:25.05 Exhaust.select :close @16:28:25.17 SSV.dial CBV @16:28:25.20 CBV.dial SSV @16:28:26.04 Allowing 130s for 25.0ml of remaining 650.0ml @16:28:26.05 Intake.select :open @16:28:28.23 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:28:28.25 SS.reconfigure SSinhale.with maxSpeed: 43 @16:28:28.30 SS.seek 25ml @16:29:08.95 delay 20 seconds @16:29:09.97 Stroke completed -- waiting for pressure to equalize ... @16:29:13.84 Intake.select :close @16:29:14.01 SSV.dial :exhaust @16:29:15.40 Exhaust.select :open @16:29:15.51 SS.reconfigure SSexhale @16:29:15.57 SS.seek 0ml @16:29:24.92 Exhaust.select :close @16:29:25.04 SSV.dial CBV @16:29:25.07 CBV.dial SSV @16:29:25.90 Allowing 130s for 25.0ml of remaining 625.0ml @16:29:25.91 Intake.select :open @16:29:28.10 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:29:28.12 SS.reconfigure SSinhale.with maxSpeed: 43 @16:29:28.17 SS.seek 25ml @16:29:28.96 ATV.dial :air3 @16:29:30.38 AS.seek 0.59ml @16:30:07.73 Vacuum too high -- waiting up to 40s @16:30:08.85 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:30:08.87 SS.reconfigure SSinhale.with maxSpeed: 39 @16:30:08.92 SS.seek 25ml @16:30:10.61 Stroke completed -- waiting for pressure to equalize ... @16:30:13.24 Intake.select :close @16:30:13.40 SSV.dial :exhaust @16:30:14.21 Exhaust.select :open @16:30:14.32 SS.reconfigure SSexhale @16:30:14.38 SS.seek 0ml @16:30:23.45 Exhaust.select :close @16:30:23.57 SSV.dial CBV @16:30:23.60 CBV.dial SSV @16:30:24.43 Allowing 130s for 25.0ml of remaining 600.0ml @16:30:24.44 Intake.select :open @16:30:26.63 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:30:26.65 SS.reconfigure SSinhale.with maxSpeed: 39 @16:30:26.70 SS.seek 25ml @16:31:12.39 Stroke completed -- waiting for pressure to equalize ... @16:31:15.64 Intake.select :close @16:31:15.81 SSV.dial :exhaust @16:31:16.62 Exhaust.select :open @16:31:16.73 SS.reconfigure SSexhale @16:31:16.78 SS.seek 0ml @16:31:25.79 Exhaust.select :close @16:31:25.90 SSV.dial CBV @16:31:25.93 CBV.dial SSV @16:31:26.77 Allowing 130s for 25.0ml of remaining 575.0ml @16:31:26.78 Intake.select :open @16:31:28.97 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:31:28.99 SS.reconfigure SSinhale.with maxSpeed: 39 @16:31:29.04 SS.seek 25ml @16:31:29.32 delay 3 seconds @16:31:32.33 ATV.dial :ASV @16:31:33.65 ASV.dial :wasteR @16:31:33.73 AS.seek 0.09ml @16:32:14.73 Stroke completed -- waiting for pressure to equalize ... @16:32:18.61 Intake.select :close @16:32:18.77 SSV.dial :exhaust @16:32:19.59 Exhaust.select :open @16:32:20.29 SS.reconfigure SSexhale @16:32:20.35 SS.seek 0ml @16:32:29.41 Exhaust.select :close @16:32:29.52 SSV.dial CBV @16:32:29.55 CBV.dial SSV @16:32:30.37 Allowing 130s for 25.0ml of remaining 550.0ml @16:32:30.38 Intake.select :open @16:32:32.56 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:32:32.58 SS.reconfigure SSinhale.with maxSpeed: 39 @16:32:32.63 SS.seek 25ml @16:33:14.56 delay 30 seconds @16:33:17.89 Stroke completed -- waiting for pressure to equalize ... @16:33:21.77 Intake.select :close @16:33:21.93 SSV.dial :exhaust @16:33:22.73 Exhaust.select :open @16:33:22.84 SS.reconfigure SSexhale @16:33:22.90 SS.seek 0ml @16:33:32.20 Exhaust.select :close @16:33:32.32 SSV.dial CBV @16:33:32.35 CBV.dial SSV @16:33:33.18 Allowing 130s for 25.0ml of remaining 525.0ml @16:33:33.19 Intake.select :open @16:33:35.37 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:33:35.39 SS.reconfigure SSinhale.with maxSpeed: 39 @16:33:35.44 SS.seek 25ml @16:33:44.58 ATV.dial :extSrc @16:33:45.32 delay 3 seconds @16:33:48.34 ATV.dial :ElutionWater @16:33:49.39 AS.seek 0.12ml @16:33:55.74 ATV.dial :air3 @16:33:56.35 AS.seek 0.13ml @16:33:58.60 delay 2 seconds @16:34:00.61 ATV.dial :bleach @16:34:01.07 AS.seek 0.23ml @16:34:20.44 Stroke completed -- waiting for pressure to equalize ... @16:34:21.45 delay 3 seconds @16:34:24.32 Intake.select :close @16:34:24.49 SSV.dial :exhaust @16:34:24.54 ATV.dial :air3 @16:34:25.07 delay 3 seconds @16:34:25.31 Exhaust.select :open @16:34:25.42 SS.reconfigure SSexhale @16:34:25.49 SS.seek 0ml @16:34:28.15 AS.seek 0.255ml @16:34:33.46 delay 3 seconds @16:34:34.23 Exhaust.select :close @16:34:34.35 SSV.dial CBV @16:34:34.38 CBV.dial SSV @16:34:35.21 Allowing 130s for 25.0ml of remaining 500.0ml @16:34:35.22 Intake.select :open @16:34:36.47 ATV.dial :bleach @16:34:37.07 AS.seek 0.24ml @16:34:37.41 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:34:37.43 SS.reconfigure SSinhale.with maxSpeed: 39 @16:34:37.48 SS.seek 25ml @16:34:40.42 delay 3 seconds @16:34:43.44 ATV.dial :ASV @16:34:44.39 ASV.dial :SPEdelivery @16:34:44.75 AFV.select :carrierSolution @16:34:44.87 delay 2 seconds @16:34:46.90 AS.reconfigure ASconfig @16:34:46.95 AS.seek 0.5ml @16:35:02.20 delay 1 second @16:35:03.21 AFV.select :holdingCoil @16:35:03.37 AS.reconfigure ASslow1 @16:35:03.42 AS.seek 0.17ml @16:35:23.19 Stroke completed -- waiting for pressure to equalize ... @16:35:26.98 Intake.select :close @16:35:27.14 SSV.dial :exhaust @16:35:27.95 Exhaust.select :open @16:35:28.06 SS.reconfigure SSexhale @16:35:28.12 SS.seek 0ml @16:35:37.22 Exhaust.select :close @16:35:37.34 SSV.dial CBV @16:35:37.37 CBV.dial SSV @16:35:38.20 Allowing 130s for 25.0ml of remaining 475.0ml @16:35:38.21 Intake.select :open @16:35:40.40 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:35:40.42 SS.reconfigure SSinhale.with maxSpeed: 39 @16:35:40.47 SS.seek 25ml @16:36:10.07 SPE.hold 2:46:40,95C @16:36:10.13 delay 3:00 @16:36:25.76 Stroke completed -- waiting for pressure to equalize ... @16:36:30.28 Intake.select :close @16:36:30.44 SSV.dial :exhaust @16:36:31.26 Exhaust.select :open @16:36:31.37 SS.reconfigure SSexhale @16:36:31.42 SS.seek 0ml @16:36:40.36 Exhaust.select :close @16:36:40.48 SSV.dial CBV @16:36:40.51 CBV.dial SSV @16:36:41.37 Allowing 130s for 25.0ml of remaining 450.0ml @16:36:41.38 Intake.select :open @16:36:43.56 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:36:43.58 SS.reconfigure SSinhale.with maxSpeed: 39 @16:36:43.63 SS.seek 25ml @16:37:29.38 Stroke completed -- waiting for pressure to equalize ... @16:37:33.90 Intake.select :close @16:37:34.07 SSV.dial :exhaust @16:37:34.91 Exhaust.select :open @16:37:35.05 SS.reconfigure SSexhale @16:37:35.10 SS.seek 0ml @16:37:44.37 Exhaust.select :close @16:37:44.48 SSV.dial CBV @16:37:44.51 CBV.dial SSV @16:37:45.37 Allowing 130s for 25.0ml of remaining 425.0ml @16:37:45.38 Intake.select :open @16:37:47.56 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:37:47.58 SS.reconfigure SSinhale.with maxSpeed: 39 @16:37:47.63 SS.seek 25ml @16:38:33.05 Stroke completed -- waiting for pressure to equalize ... @16:38:38.14 Intake.select :close @16:38:38.31 SSV.dial :exhaust @16:38:39.15 Exhaust.select :open @16:38:39.26 SS.reconfigure SSexhale @16:38:39.32 SS.seek 0ml @16:38:48.29 Exhaust.select :close @16:38:48.40 SSV.dial CBV @16:38:48.43 CBV.dial SSV @16:38:49.27 Allowing 130s for 25.0ml of remaining 400.0ml @16:38:49.28 Intake.select :open @16:38:51.46 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:38:51.48 SS.reconfigure SSinhale.with maxSpeed: 39 @16:38:51.53 SS.seek 25ml @16:39:10.17 AFV.select :carrierSolution @16:39:10.28 delay 2 seconds @16:39:12.31 AS.reconfigure ASconfig @16:39:12.36 AS.seek 1ml @16:39:37.28 Stroke completed -- waiting for pressure to equalize ... @16:39:41.81 Intake.select :close @16:39:41.98 SSV.dial :exhaust @16:39:42.80 Exhaust.select :open @16:39:42.92 SS.reconfigure SSexhale @16:39:42.98 SS.seek 0ml @16:39:52.15 Exhaust.select :close @16:39:52.27 SSV.dial CBV @16:39:52.30 CBV.dial SSV @16:39:53.13 Allowing 130s for 25.0ml of remaining 375.0ml @16:39:53.14 Intake.select :open @16:39:55.32 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:39:55.34 SS.reconfigure SSinhale.with maxSpeed: 39 @16:39:55.39 SS.seek 25ml @16:40:00.36 delay 1 second @16:40:01.37 AFV.select :holdingCoil @16:40:01.48 delay 1 second @16:40:02.49 ASV.dial :wasteD @16:40:03.48 AS.seek 0ml @16:40:37.79 Vacuum too high -- waiting up to 40s @16:40:39.17 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:40:39.19 SS.reconfigure SSinhale.with maxSpeed: 35 @16:40:39.24 SS.seek 25ml @16:40:42.82 Stroke completed -- waiting for pressure to equalize ... @16:40:46.06 Intake.select :close @16:40:46.22 SSV.dial :exhaust @16:40:47.02 Exhaust.select :open @16:40:47.13 SS.reconfigure SSexhale @16:40:47.19 SS.seek 0ml @16:40:56.08 Exhaust.select :close @16:40:56.20 SSV.dial CBV @16:40:56.23 CBV.dial SSV @16:40:57.07 Allowing 130s for 25.0ml of remaining 350.0ml @16:40:57.08 Intake.select :open @16:40:59.26 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:40:59.28 SS.reconfigure SSinhale.with maxSpeed: 35 @16:40:59.33 SS.seek 25ml @16:41:01.27 Priming Eluate @16:41:01.28 AFV.select :carrierSolution @16:41:01.39 delay 2 seconds @16:41:03.41 AS.seek 0.5ml @16:41:32.47 delay 1 second @16:41:33.48 AFV.select :holdingCoil @16:41:33.59 ATV.dial ASV @16:41:33.60 ASV.dial :SPErecovery @16:41:34.15 Swish #1 of 2 @16:41:34.80 AS.reconfigure ASslow0 @16:41:34.85 AS.seek 0.6ml @16:41:45.33 AS.seek 0.5ml @16:41:50.06 Stroke completed -- waiting for pressure to equalize ... @16:41:53.96 Intake.select :close @16:41:54.13 SSV.dial :exhaust @16:41:54.96 Exhaust.select :open @16:41:55.07 SS.reconfigure SSexhale @16:41:55.13 SS.seek 0ml @16:41:55.73 Swish #2 of 2 @16:41:55.82 AS.seek 0.6ml @16:42:04.40 Exhaust.select :close @16:42:04.52 SSV.dial CBV @16:42:04.55 CBV.dial SSV @16:42:05.38 Allowing 130s for 25.0ml of remaining 325.0ml @16:42:05.39 Intake.select :open @16:42:06.29 AS.seek 0.5ml @16:42:07.57 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:42:07.59 SS.reconfigure SSinhale.with maxSpeed: 35 @16:42:07.64 SS.seek 25ml @16:42:16.69 delay 3 seconds @16:42:19.78 AS.reconfigure ASslow1 @16:42:19.83 AS.seek 0.684ml @16:42:57.17 delay 3 seconds @16:42:58.03 Stroke completed -- waiting for pressure to equalize ... @16:43:00.19 Eluate primed @16:43:00.20 ATV.dial :waste2 @16:43:00.89 AS.reconfigure ASnorm @16:43:00.94 AS.seek 0ml @16:43:01.92 Intake.select :close @16:43:02.08 SSV.dial :exhaust @16:43:02.91 Exhaust.select :open @16:43:03.03 SS.reconfigure SSexhale @16:43:03.08 SS.seek 0ml @16:43:12.47 Exhaust.select :close @16:43:12.59 SSV.dial CBV @16:43:12.62 CBV.dial SSV @16:43:13.45 Allowing 130s for 25.0ml of remaining 300.0ml @16:43:13.46 Intake.select :open @16:43:15.65 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:43:15.67 SS.reconfigure SSinhale.with maxSpeed: 35 @16:43:15.72 SS.seek 25ml @16:43:35.72 SPE.coast @16:43:35.81 ASV.dial :SPErecovery @16:43:35.83 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0716h1500ml.pcr @16:43:35.85 Building PCR Slug from ASV with MM2 mix, PP4 primer @16:43:35.86 Priming with carrier @16:43:35.87 ATV.dial ARV @16:43:36.78 ARV.dial :carrier @16:43:37.13 AS.seek 0.5ml @16:44:02.71 AFV.select :carrierSolution @16:44:02.83 delay 2 seconds @16:44:04.86 AS.reconfigure ASconfig @16:44:04.91 AS.seek 1ml @16:44:06.05 Stroke completed -- waiting for pressure to equalize ... @16:44:09.95 Intake.select :close @16:44:10.12 SSV.dial :exhaust @16:44:11.51 Exhaust.select :open @16:44:11.62 SS.reconfigure SSexhale @16:44:11.68 SS.seek 0ml @16:44:20.94 Exhaust.select :close @16:44:21.06 SSV.dial CBV @16:44:21.09 CBV.dial SSV @16:44:21.91 Allowing 130s for 25.0ml of remaining 275.0ml @16:44:21.92 Intake.select :open @16:44:24.10 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:44:24.12 SS.reconfigure SSinhale.with maxSpeed: 35 @16:44:24.17 SS.seek 25ml @16:44:33.92 delay 1 second @16:44:34.94 AFV.select :holdingCoil @16:44:35.05 delay 1 second @16:44:36.07 ATV.dial :waste @16:44:37.47 AS.reconfigure ASnorm @16:44:37.52 AS.seek :empty @16:45:14.58 Stroke completed -- waiting for pressure to equalize ... @16:45:19.09 Intake.select :close @16:45:19.25 SSV.dial :exhaust @16:45:20.04 Exhaust.select :open @16:45:20.15 SS.reconfigure SSexhale @16:45:20.21 SS.seek 0ml @16:45:28.27 AFV.select :carrierSolution @16:45:28.39 delay 2 seconds @16:45:29.65 Exhaust.select :close @16:45:29.77 SSV.dial CBV @16:45:29.80 CBV.dial SSV @16:45:30.42 AS.reconfigure ASconfig @16:45:30.47 AS.seek 0.5ml @16:45:30.62 Allowing 130s for 25.0ml of remaining 250.0ml @16:45:30.63 Intake.select :open @16:45:32.82 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:45:32.84 SS.reconfigure SSinhale.with maxSpeed: 35 @16:45:32.89 SS.seek 25ml @16:45:59.56 delay 1 second @16:46:00.58 AFV.select :holdingCoil @16:46:00.69 Building Slug @16:46:00.70 ATV.dial :ARV @16:46:02.22 delay 2 seconds @16:46:04.24 ARV.dial :air1 @16:46:04.63 delay 2 seconds @16:46:06.72 AS.reconfigure ASslow2 @16:46:06.77 AS.seek 0.506ml @16:46:13.00 delay 2 seconds @16:46:15.03 ARV.dial :MM2 @16:46:16.41 delay 2 seconds @16:46:18.44 Adding MM2 @16:46:18.52 AS.seek 0.524ml @16:46:23.39 Stroke completed -- waiting for pressure to equalize ... @16:46:27.92 Intake.select :close @16:46:28.08 SSV.dial :exhaust @16:46:28.87 Exhaust.select :open @16:46:28.98 SS.reconfigure SSexhale @16:46:29.04 SS.seek 0ml @16:46:37.01 delay 25 seconds @16:46:38.12 Exhaust.select :close @16:46:38.24 SSV.dial CBV @16:46:38.27 CBV.dial SSV @16:46:39.07 Allowing 130s for 25.0ml of remaining 225.0ml @16:46:39.08 Intake.select :open @16:46:41.26 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:46:41.28 SS.reconfigure SSinhale.with maxSpeed: 35 @16:46:41.33 SS.seek 25ml @16:47:02.04 ARV.dial :PP4 @16:47:03.00 Adding PP4 @16:47:03.01 delay 2 seconds @16:47:05.10 AS.seek 0.53ml @16:47:11.58 delay 10 seconds @16:47:21.60 ARV.dial :air3 @16:47:22.00 adding ARV air @16:47:22.02 delay 2 seconds @16:47:24.10 AS.seek 0.536ml @16:47:30.33 delay 5 seconds @16:47:31.67 Stroke completed -- waiting for pressure to equalize ... @16:47:35.34 ARV.dial :carrier @16:47:36.22 Intake.select :close @16:47:36.38 SSV.dial :exhaust @16:47:36.53 delay 7 seconds @16:47:37.20 Exhaust.select :open @16:47:37.31 SS.reconfigure SSexhale @16:47:37.37 SS.seek 0ml @16:47:43.62 AS.seek 0.594ml @16:47:46.53 Exhaust.select :close @16:47:46.66 SSV.dial CBV @16:47:46.72 CBV.dial SSV @16:47:47.57 Allowing 130s for 25.0ml of remaining 200.0ml @16:47:47.58 Intake.select :open @16:47:49.77 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:47:49.79 SS.reconfigure SSinhale.with maxSpeed: 35 @16:47:49.84 SS.seek 25ml @16:48:40.19 Stroke completed -- waiting for pressure to equalize ... @16:48:42.15 delay 5 seconds @16:48:45.49 Intake.select :close @16:48:45.65 SSV.dial :exhaust @16:48:46.45 Exhaust.select :open @16:48:46.56 SS.reconfigure SSexhale @16:48:46.61 SS.seek 0ml @16:48:47.17 ATV.dial :ASV @16:48:47.55 delay 2 seconds @16:48:49.64 AS.seek 0.6ml @16:48:55.95 delay 4 seconds @16:48:56.03 Exhaust.select :close @16:48:56.15 SSV.dial CBV @16:48:56.18 CBV.dial SSV @16:48:56.99 Allowing 130s for 25.0ml of remaining 175.0ml @16:48:57.00 Intake.select :open @16:48:59.19 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:48:59.21 SS.reconfigure SSinhale.with maxSpeed: 35 @16:48:59.26 SS.seek 25ml @16:48:59.96 ATV.dial ARV @16:49:00.59 delay 2 seconds @16:49:02.61 ARV.dial :carrier @16:49:02.63 delay 2 seconds @16:49:04.72 AS.seek 0.65ml @16:49:49.79 Stroke completed -- waiting for pressure to equalize ... @16:49:54.92 Intake.select :close @16:49:55.08 SSV.dial :exhaust @16:49:55.39 delay 4 seconds @16:49:55.90 Exhaust.select :open @16:49:56.02 SS.reconfigure SSexhale @16:49:56.08 SS.seek 0ml @16:49:59.40 ATV.dial PCR @16:50:00.09 ASV.dial :waste1 @16:50:00.92 ARV.dial :air1 @16:50:01.30 Positioning Slug by 0.324ml @16:50:01.39 AS.seek 0.326ml @16:50:05.01 Exhaust.select :close @16:50:05.12 SSV.dial CBV @16:50:05.15 CBV.dial SSV @16:50:05.99 Allowing 130s for 25.0ml of remaining 150.0ml @16:50:06.00 Intake.select :open @16:50:08.18 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:50:08.20 SS.reconfigure SSinhale.with maxSpeed: 35 @16:50:08.25 SS.seek 25ml @16:50:58.97 Stroke completed -- waiting for pressure to equalize ... @16:51:04.16 Intake.select :close @16:51:04.32 SSV.dial :exhaust @16:51:05.12 Exhaust.select :open @16:51:05.23 SS.reconfigure SSexhale @16:51:05.28 SS.seek 0ml @16:51:14.38 Exhaust.select :close @16:51:14.49 SSV.dial CBV @16:51:14.52 CBV.dial SSV @16:51:15.32 Allowing 130s for 25.0ml of remaining 125.0ml @16:51:15.33 Intake.select :open @16:51:17.51 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:51:17.53 SS.reconfigure SSinhale.with maxSpeed: 35 @16:51:17.58 SS.seek 25ml @16:51:58.07 Vacuum too high -- waiting up to 40s @16:51:59.46 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:51:59.48 SS.reconfigure SSinhale.with maxSpeed: 32 @16:51:59.53 SS.seek 25ml @16:52:10.42 Stroke completed -- waiting for pressure to equalize ... @16:52:14.93 Intake.select :close @16:52:15.09 SSV.dial :exhaust @16:52:15.90 Exhaust.select :open @16:52:16.02 SS.reconfigure SSexhale @16:52:16.07 SS.seek 0ml @16:52:25.54 Exhaust.select :close @16:52:25.66 SSV.dial CBV @16:52:25.69 CBV.dial SSV @16:52:26.52 Allowing 130s for 25.0ml of remaining 100.0ml @16:52:26.53 Intake.select :open @16:52:28.72 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:52:28.74 SS.reconfigure SSinhale.with maxSpeed: 32 @16:52:29.40 SS.seek 25ml @16:53:24.41 Stroke completed -- waiting for pressure to equalize ... @16:53:28.96 Intake.select :close @16:53:29.12 SSV.dial :exhaust @16:53:29.92 Exhaust.select :open @16:53:30.04 SS.reconfigure SSexhale @16:53:30.10 SS.seek 0ml @16:53:39.41 Exhaust.select :close @16:53:39.52 SSV.dial CBV @16:53:39.55 CBV.dial SSV @16:53:40.35 Allowing 130s for 25.0ml of remaining 75.0ml @16:53:40.36 Intake.select :open @16:53:42.54 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:53:42.56 SS.reconfigure SSinhale.with maxSpeed: 32 @16:53:42.61 SS.seek 25ml @16:54:37.70 Stroke completed -- waiting for pressure to equalize ... @16:54:42.85 Intake.select :close @16:54:43.02 SSV.dial :exhaust @16:54:43.84 Exhaust.select :open @16:54:43.95 SS.reconfigure SSexhale @16:54:44.01 SS.seek 0ml @16:54:52.86 Exhaust.select :close @16:54:52.98 SSV.dial CBV @16:54:53.01 CBV.dial SSV @16:54:53.84 Allowing 130s for 25.0ml of remaining 50.0ml @16:54:53.85 Intake.select :open @16:54:56.03 Intake @35.0psia, vacuum from 20.0 to 24.0psi @16:54:56.05 SS.reconfigure SSinhale.with maxSpeed: 32 @16:54:56.10 SS.seek 25ml @16:55:27.42 delay 5 seconds @16:55:32.44 ATV.dial ARV @16:55:33.16 ASV.dial :PCRlock @16:55:33.61 delay 5 seconds @16:55:38.64 Running Tt1 Profile60C300 thermocycling @16:55:38.65 PCR.program Profile60C300 @16:55:38.79 Profile60C300 should complete in < 1:45:21 @16:55:38.80 PCR.run 2:16:41.25 @16:55:51.45 Stroke completed -- waiting for pressure to equalize ... @16:55:56.60 Intake.select :close @16:55:56.76 SSV.dial :exhaust @16:55:57.57 Exhaust.select :open @16:55:57.69 SS.reconfigure SSexhale @16:55:57.75 SS.seek 0ml @16:56:06.92 Exhaust.select :close @16:56:07.04 SSV.dial CBV @16:56:07.07 CBV.dial SSV @16:56:07.90 Allowing 130s for 25.0ml of remaining 25.0ml @16:56:07.91 Intake.select :open @16:56:10.69 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:56:10.71 SS.reconfigure SSinhale.with maxSpeed: 32 @16:56:10.76 SS.seek 24.96ml @16:57:05.45 Stroke completed -- waiting for pressure to equalize ... @16:57:10.62 Intake.select :close @16:57:10.79 SSV.dial :exhaust @16:57:11.59 Exhaust.select :open @16:57:11.70 SS.reconfigure SSexhale @16:57:11.76 SS.seek 0ml @16:57:21.06 Exhaust.select :close @16:57:21.18 Sampled 1500.0ml @16:57:21.20 Drying puck before fixing for 4:00 @16:57:21.21 CBV.dial :waste2b @16:57:22.02 delay 1:00 @16:58:22.04 CSV.dial :air @16:58:22.51 CS.reconfigure CSfast @16:58:22.57 CS.seek 10ml @16:58:46.71 Pumping wcr puck from tube 3 up to 55psi... @16:58:46.72 CBV.dial SSV @16:58:48.10 CBV.dial between CSV and waste2b @16:58:49.13 CSV.dial between air and CTV @16:58:49.49 CS.seek 6.959ml @16:58:57.46 CTV.dial CSV,avoiding: :air @16:58:57.49 CSV.dial CTV @16:58:58.34 CS.reconfigure CSpressurize @16:58:58.40 CS.seek 0ml @16:59:16.73 CSV.dial :air @16:59:17.21 CS.reconfigure CSfast @16:59:17.27 CS.seek 10ml @16:59:41.21 CSV.dial between air and CTV @16:59:41.57 CS.seek 2.459ml @16:59:59.85 CTV.dial CSV,avoiding: :air @16:59:59.88 CSV.dial CTV @17:00:00.20 CS.reconfigure CSpressurize @17:00:00.26 CS.seek 0ml @17:00:04.64 CBV.dial :waste2b @17:00:05.00 CS.reconfigure CSconfig @17:00:05.65 CS.seek :empty @17:00:09.21 delay 1:00 @17:01:09.24 CTV.dial :air,avoiding: :intake @17:01:10.59 Fix #1 of 2 (with rnal) for 2:00 @17:01:10.61 CSV.dial CSR @17:01:11.13 Pulling 2ml of CSR.rnal @17:01:11.15 CSR.select :rnal @17:01:11.27 CS.seek 2ml @17:01:17.40 delay 5 seconds @17:01:22.42 CSR.select :air @17:01:22.55 CS.reconfigure CSfast @17:01:22.60 CS.seek 10ml @17:01:41.81 Pumping wcr puck from tube 3 up to 55psi... @17:01:41.82 CBV.dial SSV @17:01:43.17 CBV.dial between CSV and waste2b @17:01:44.19 CSV.dial between air and CTV @17:01:44.97 CS.seek 6.839ml @17:01:53.18 CTV.dial CSV,avoiding: :air @17:01:53.21 CSV.dial CTV @17:01:54.65 CS.reconfigure CSpressurize @17:01:54.71 CS.seek 0ml @17:02:12.87 CSV.dial :air @17:02:13.36 CS.reconfigure CSfast @17:02:13.42 CS.seek 10ml @17:02:37.34 CSV.dial between air and CTV @17:02:37.71 CS.seek 1.988ml @17:02:57.12 CTV.dial CSV,avoiding: :air @17:02:57.15 CSV.dial CTV @17:02:57.47 CS.reconfigure CSpressurize @17:02:57.53 CS.seek 0ml @17:02:58.74 delay 2:00 @17:04:58.76 CBV.dial :waste2b @17:04:59.13 CS.reconfigure CSconfig @17:04:59.18 CS.seek :empty @17:05:04.53 delay 1:00 @17:06:04.55 CTV.dial :air,avoiding: :intake @17:06:05.91 Fix #2 of 2 (with rnal) for 2:00 @17:06:05.93 CSV.dial CSR @17:06:07.05 Pulling 2ml of CSR.rnal @17:06:07.06 CSR.select :rnal @17:06:07.19 CS.seek 2ml @17:06:13.28 delay 5 seconds @17:06:18.30 CSR.select :air @17:06:18.43 CS.reconfigure CSfast @17:06:18.49 CS.seek 10ml @17:06:37.81 Pumping wcr puck from tube 3 up to 55psi... @17:06:37.82 CBV.dial SSV @17:06:39.18 CBV.dial between CSV and waste2b @17:06:40.18 CSV.dial between air and CTV @17:06:40.82 CS.seek 6.816ml @17:06:49.17 CTV.dial CSV,avoiding: :air @17:06:49.19 CSV.dial CTV @17:06:50.63 CS.reconfigure CSpressurize @17:06:50.69 CS.seek 0ml @17:07:08.77 CSV.dial :air @17:07:09.27 CS.reconfigure CSfast @17:07:09.33 CS.seek 10ml @17:07:33.04 CSV.dial between air and CTV @17:07:33.39 CS.seek 1.985ml @17:07:52.91 CTV.dial CSV,avoiding: :air @17:07:52.94 CSV.dial CTV @17:07:53.27 CS.reconfigure CSpressurize @17:07:53.33 CS.seek 0ml @17:07:54.49 delay 2:00 @17:09:54.52 CBV.dial :waste2b @17:09:54.86 CS.reconfigure CSconfig @17:09:54.91 CS.seek :empty @17:10:00.47 delay 1:00 @17:11:00.50 CTV.dial :air,avoiding: :intake @17:11:01.92 Storing wcr puck in Collection Clamp to tube 2 @17:11:02.12 SC.reconfigure SCtight @17:11:02.17 SC.seek 2 @17:11:04.32 SC.reconfigure SCloose @17:11:04.56 Elbow.reconfigure TightElbow @17:11:04.61 Elbow.seek 2 @17:11:07.11 Forearm.reconfigure TightForearm @17:11:07.16 Forearm.seek 2 + 700 counts @17:11:08.32 SE.reconfigure SEpick @17:11:08.40 SE.seek beamBreak @17:11:11.35 SE.configure SEconfig @17:11:11.53 Elbow.reconfigure LimpElbow @17:11:11.59 Forearm.reconfigure LooseForearm @17:11:11.64 Forearm.seek 2 @17:11:11.97 Elbow.hold @17:11:12.05 Elbow.reconfigure TightElbow @17:11:12.16 SE.seek 0.06 Pucks below Top Plate @17:11:12.62 Forearm.force -20 @17:11:12.67 Forearm.seek 2 + 314 counts @17:11:13.01 Forearm.reconfigure TightForearm @17:11:13.07 Forearm.seek 2 + 700 counts @17:11:13.40 SE.seek 1.06 Pucks below Top Plate @17:11:14.02 Forearm.reconfigure LooseForearm @17:11:14.07 Forearm.seek :retract @17:11:15.02 Elbow.seek CC @17:11:16.67 Elbow.reconfigure LooseElbow @17:11:16.78 Forearm.seek CC @17:11:17.76 Hand.close @17:11:18.34 CC.seek :open @17:11:22.87 Forearm.seek :retract @17:11:23.83 Elbow.reconfigure TightElbow @17:11:23.88 Elbow.seek 2 @17:11:25.73 Elbow.reconfigure LimpElbow @17:11:25.79 Forearm.seek 2 @17:11:26.80 Elbow.hold @17:11:26.88 Elbow.reconfigure TightElbow @17:11:26.93 Hand.open @17:11:27.75 Forearm.force -20 @17:11:27.80 Forearm.seek 2 + 340 counts @17:11:28.14 Forearm.reconfigure TightForearm @17:11:28.20 Forearm.seek 2 + 700 counts @17:11:28.64 SE.seek :down @17:11:29.80 Forearm.reconfigure LooseForearm @17:11:29.85 Forearm.seek :retract @17:11:31.39 SE.coast @17:11:31.43 SC.coast @17:11:31.48 Starting finalPurge @17:11:32.09 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:11:32.17 Hand.open @17:11:32.61 Elbow.seek FlushPuck::Garage @17:11:34.63 Elbow.reconfigure LooseElbow @17:11:34.73 Forearm.seek :garage @17:11:35.72 Hand.close @17:11:36.48 Forearm.seek :retract @17:11:37.47 Elbow.reconfigure TightElbow @17:11:37.52 Elbow.seek CC @17:11:40.45 Elbow.reconfigure LooseElbow @17:11:40.55 Forearm.reconfigure TightForearm @17:11:40.60 Forearm.seek CC @17:11:41.89 CC.seek :closed @17:11:46.15 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.98 @17:11:46.24 Hand.open @17:11:46.91 Forearm.reconfigure LooseForearm @17:11:46.96 Forearm.seek :retract @17:11:47.97 Flushing the Collection Valve::Manifold @17:11:48.11 CSV.dial CSR @17:11:48.64 Pulling 5ml of CSR.flush @17:11:48.65 CSR.select :flush @17:11:48.78 CS.seek 5ml @17:12:02.51 delay 5 seconds @17:12:07.53 CSR.select :air @17:12:07.70 CS.reconfigure CSfast @17:12:07.75 CS.seek 7.5ml @17:12:14.51 CSV.dial :waste @17:12:15.85 CS.reconfigure CSconfig @17:12:15.90 CS.seek 0ml @17:12:35.98 Flushing lower manifold's waste1 port @17:12:36.12 CSV.dial :flush @17:12:36.58 CS.reconfigure CSfast @17:12:36.63 CS.seek 5ml @17:12:48.98 delay 5 seconds @17:12:53.99 CSV.dial :air @17:12:54.55 CS.seek 7ml @17:13:00.10 CSV.dial CTV @17:13:00.15
CTV.dial CSV @17:13:01.55 CBV.dial :waste1 @17:13:02.36 CS.seek 0ml @17:13:19.29 Flushing lower manifold's waste2b port @17:13:19.43 CSV.dial :flush @17:13:20.22 CS.seek 5ml @17:13:32.66 delay 5 seconds @17:13:37.67 CSV.dial :air @17:13:38.23 CS.seek 7ml @17:13:43.79 CSV.dial CTV @17:13:43.82
CTV.dial CSV @17:13:44.29 CBV.dial :waste2b @17:13:45.11 CS.seek 0ml @17:14:01.98 Flushing upper manifold @17:14:02.12 CSV.dial :flush @17:14:02.91 CS.seek 5ml @17:14:15.41 delay 5 seconds @17:14:20.43 CSV.dial :air @17:14:20.98 CS.seek 7ml @17:14:26.57 CSV.dial CBV @17:14:26.60
CBV.dial CSV @17:14:27.76 CTV.dial :air @17:14:29.13 CS.seek 0ml @17:14:45.98 delay 7 seconds @17:14:53.00 CS.seek 3ml @17:15:00.84 CSV.dial :waste @17:15:01.33 CS.seek 0ml @17:15:09.23 CSV.dial :flush @17:15:09.71 CS.seek 3ml @17:15:17.57 delay 5 seconds @17:15:22.58 CSV.dial :air @17:15:23.13 CS.seek 5ml @17:15:28.78 CSV.dial :air @17:15:28.81 CS.seek 0ml @17:15:41.17 delay 7 seconds @17:15:48.19 CS.seek 3ml @17:15:56.12 CSV.dial :waste @17:15:56.91 CS.seek 0ml @17:16:04.88 Disinfecting external Intake with mfbkill @17:16:05.02 CSV.dial CSR @17:16:06.43 Pulling 8ml of CSR.mfbkill @17:16:06.44 CSR.select :mfbkill @17:16:06.57 CS.seek 8ml @17:16:25.79 delay 5 seconds @17:16:30.80 CSR.select :air @17:16:30.93 CS.seek 10ml @17:16:36.51 CSV.dial CBV @17:16:36.54
CBV.dial CSV @17:16:37.60 CTV.dial :intake @17:16:38.37 Intake.select :open @17:16:38.49 CS.seek 2ml @17:16:58.38 Intake.select :close @17:16:58.50 CTV.dial :air @17:16:59.29 CS.reconfigure CSconfig @17:16:59.34 CS.seek 0ml @17:17:05.51 Flushing lower manifold's waste1 port @17:17:05.52 CSV.dial CSR @17:17:06.64 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @17:17:06.77 CS.seek 4ml @17:17:18.04 delay 5 seconds @17:17:23.05 CSR.select :air @17:17:23.18 CS.reconfigure CSfast @17:17:23.23 CS.seek 10ml @17:17:37.85 CSV.dial CTV @17:17:37.88
CTV.dial CSV @17:17:39.34 CBV.dial :waste1 @17:17:39.83 CS.seek 0ml @17:18:03.57 Flushing lower manifold's waste2b port @17:18:03.58 CSV.dial CSR @17:18:04.12 Pulling 4ml of CSR.mfbkill @17:18:04.13 CSR.select :mfbkill @17:18:04.26 CS.reconfigure CSconfig @17:18:04.30 CS.seek 4ml @17:18:15.67 delay 5 seconds @17:18:20.69 CSR.select :air @17:18:20.82 CS.reconfigure CSfast @17:18:20.87 CS.seek 10ml @17:18:35.61 CSV.dial CTV @17:18:35.64
CTV.dial CSV @17:18:36.14 CBV.dial :waste2b @17:18:36.94 CS.seek 0ml @17:19:00.54 Killing upper manifold @17:19:00.55 CSV.dial CSR @17:19:01.09 Pulling 4ml of CSR.mfbkill @17:19:01.10 CSR.select :mfbkill @17:19:01.23 CS.reconfigure CSconfig @17:19:01.27 CS.seek 4ml @17:19:12.54 delay 5 seconds @17:19:17.56 CSR.select :air @17:19:17.69 CS.reconfigure CSfast @17:19:17.74 CS.seek 10ml @17:19:32.36 CSV.dial CBV @17:19:32.39
CBV.dial CSV @17:19:33.48 CTV.dial :air @17:19:34.87 CS.seek 0ml @17:19:58.54 delay 7 seconds @17:20:05.57 CS.seek 3ml @17:20:13.50 CSV.dial :waste @17:20:14.10 CS.seek 0ml @17:20:22.08 Flushing upper manifold @17:20:22.22 CSV.dial :flush @17:20:22.71 CS.seek 5ml @17:20:35.04 delay 5 seconds @17:20:40.05 CSV.dial :air @17:20:40.60 CS.seek 7ml @17:20:46.26 CSV.dial CBV @17:20:46.29
CBV.dial CSV @17:20:47.37 CTV.dial :air @17:20:47.39 CS.seek 0ml @17:21:04.36 delay 7 seconds @17:21:11.38 CS.seek 3ml @17:21:19.22 CSV.dial :waste @17:21:19.71 CS.seek 0ml @17:21:27.60 CSV.dial :flush @17:21:28.09 CS.seek 3ml @17:21:35.92 delay 5 seconds @17:21:40.93 CSV.dial :air @17:21:41.47 CS.seek 5ml @17:21:47.00 CSV.dial :air @17:21:47.62 CS.seek 0ml @17:22:00.11 delay 7 seconds @17:22:07.14 CS.seek 3ml @17:22:14.94 CSV.dial :waste @17:22:15.73 CS.seek 0ml @17:22:23.51 Flushing lower manifold's waste1 port @17:22:23.65 CSV.dial :flush @17:22:24.15 CS.seek 5ml @17:22:36.61 delay 5 seconds @17:22:41.62 CSV.dial :air @17:22:42.18 CS.seek 7ml @17:22:47.89 CSV.dial CTV @17:22:47.92
CTV.dial CSV @17:22:49.37 CBV.dial :waste1 @17:22:49.87 CS.seek 0ml @17:23:06.64 Flushing lower manifold's waste2b port @17:23:06.78 CSV.dial :flush @17:23:07.57 CS.seek 5ml @17:23:19.89 delay 5 seconds @17:23:24.91 CSV.dial :air @17:23:25.46 CS.seek 7ml @17:23:31.17 CSV.dial CTV @17:23:31.20
CTV.dial CSV @17:23:31.67 CBV.dial :waste2b @17:23:32.49 CS.seek 0ml @17:23:49.33 Disinfecting external Intake with kill @17:23:49.47 CSV.dial CSR @17:23:50.00 Pulling 8ml of CSR.kill @17:23:50.01 CSR.select :kill @17:23:50.14 CS.seek 8ml @17:24:09.39 delay 5 seconds @17:24:14.41 CSR.select :air @17:24:14.55 CS.seek 10ml @17:24:20.20 CSV.dial CBV @17:24:20.23
CBV.dial CSV @17:24:21.33 CTV.dial :intake @17:24:22.11 Intake.select :open @17:24:22.23 CS.seek 2ml @17:24:42.26 Intake.select :close @17:24:42.38 CTV.dial :air @17:24:43.16 CS.reconfigure CSconfig @17:24:43.21 CS.seek 0ml @17:24:49.39 CSV.dial CSR @17:24:50.51 Pulling 5ml of CSR.flush @17:24:50.52 CSR.select :flush @17:24:50.65 CS.seek 5ml @17:25:04.33 delay 5 seconds @17:25:09.35 CSR.select :air @17:25:09.52 CS.seek 10ml @17:25:23.32 CSV.dial CBV @17:25:24.40 CS.seek 0ml @17:25:51.08 Garaging FlushPuck in Collection Clamp @17:25:51.16 Elbow.reconfigure TightElbow @17:25:51.21 Elbow.seek CC @17:25:51.46 Elbow.reconfigure LooseElbow @17:25:51.57 Forearm.seek CC @17:25:53.15 Hand.close @17:25:53.74 CC.seek :open @17:25:58.13 Forearm.seek :retract @17:25:59.11 Elbow.reconfigure TightElbow @17:25:59.16 Elbow.seek :garage @17:26:02.39 Elbow.reconfigure LooseElbow @17:26:02.49 Forearm.reconfigure TightForearm @17:26:02.54 Forearm.seek :garage @17:26:03.69 Hand.open @17:26:04.43 Forearm.reconfigure LooseForearm @17:26:04.48 Forearm.seek :retract @17:26:05.52 Starting initialPurge @17:26:05.53 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:26:05.61 Hand.open @17:26:05.93 Elbow.reconfigure TightElbow @17:26:05.98 Elbow.seek FlushPuck::Garage @17:26:06.11 Elbow.reconfigure LooseElbow @17:26:06.22 Forearm.seek :garage @17:26:07.26 Hand.close @17:26:08.00 Forearm.seek :retract @17:26:09.04 Elbow.reconfigure TightElbow @17:26:09.09 Elbow.seek CC @17:26:11.92 Elbow.reconfigure LooseElbow @17:26:12.03 Forearm.reconfigure TightForearm @17:26:12.08 Forearm.seek CC @17:26:13.41 CC.seek :closed @17:26:17.65 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.95 @17:26:17.75 Hand.open @17:26:18.43 Forearm.reconfigure LooseForearm @17:26:18.48 Forearm.seek :retract @17:26:19.50 Flushing the Collection Valve::Manifold @17:26:19.64 CSV.dial CSR @17:26:20.75 Pulling 5ml of CSR.flush @17:26:20.76 CSR.select :flush @17:26:20.89 CS.seek 5ml @17:26:34.61 delay 5 seconds @17:26:39.63 CSR.select :air @17:26:39.81 CS.reconfigure CSfast @17:26:39.86 CS.seek 7.5ml @17:26:46.79 CSV.dial :waste @17:26:48.14 CS.reconfigure CSconfig @17:26:48.19 CS.seek 0ml @17:27:08.49 Purging external Intake @17:27:08.64 CSV.dial :flush @17:27:09.13 CS.reconfigure CSfast @17:27:09.18 CS.seek 10ml @17:27:32.89 delay 5 seconds @17:27:37.91 CSV.dial CBV @17:27:37.95
CBV.dial CSV @17:27:38.74 CTV.dial :intake @17:27:40.14 Intake.select :open @17:27:40.26 CS.seek 0ml @17:28:05.01 Intake.select :close @17:28:05.13 CTV.dial :air @17:28:05.93 Flushing upper manifold @17:28:06.07 CSV.dial :flush @17:28:06.84 CS.seek 5ml @17:28:19.27 delay 5 seconds @17:28:24.29 CSV.dial :air @17:28:24.82 CS.seek 7ml @17:28:30.36 CSV.dial CBV @17:28:30.39
CBV.dial CSV @17:28:31.47 CTV.dial :air @17:28:31.49 CS.seek 0ml @17:28:48.46 delay 7 seconds @17:28:55.48 CS.seek 3ml @17:29:03.26 CSV.dial :waste @17:29:03.75 CS.seek 0ml @17:29:11.70 CSV.dial :flush @17:29:12.17 CS.seek 3ml @17:29:19.93 delay 5 seconds @17:29:24.95 CSV.dial :air @17:29:25.53 CS.seek 5ml @17:29:31.14 CSV.dial :air @17:29:31.17 CS.seek 0ml @17:29:43.68 delay 7 seconds @17:29:50.71 CS.seek 3ml @17:29:58.51 CSV.dial :waste @17:29:59.28 CS.seek 0ml @17:30:07.15 Garaging FlushPuck in Collection Clamp @17:30:07.23 Elbow.reconfigure TightElbow @17:30:07.28 Elbow.seek CC @17:30:07.52 Elbow.reconfigure LooseElbow @17:30:07.63 Forearm.seek CC @17:30:08.61 Hand.close @17:30:09.25 CC.seek :open @17:30:13.62 Forearm.seek :retract @17:30:14.60 Elbow.reconfigure TightElbow @17:30:14.65 Elbow.seek :garage @17:30:17.73 Elbow.reconfigure LooseElbow @17:30:17.84 Forearm.reconfigure TightForearm @17:30:17.89 Forearm.seek :garage @17:30:19.04 Hand.open @17:30:19.69 Forearm.reconfigure LooseForearm @17:30:19.74 Forearm.seek :retract @17:30:20.77 Completed [phasewithdil] begun 14:00:26 @17:30:20.79 Preparing to sleep until 14PM @17:30:20.83 deferred until MFBPCR finish @17:30:20.86
Waiting for MFBPCR til 14PM @17:30:20.88 Continuing at 14:00CEST08-Oct-18 @17:30:20.94 GoodNews.email "Then, in tube 3: phasewithdil\n/home/esp/esp2/mission/18tundeployv2dil.rb:118\n{1 => 22, 2 => 2, 3 => 20}\nCan@17:30:20, 14.8C, 68% humidity, 14.3psia, 13.387V, 0.954A, 0.954A avg, 12.77W",Subject: "Waiting until 14:00CEST08-Oct-18" @18:17:59.05 PCR.off @18:18:00.61 PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0716h1500ml.pcr @18:18:00.69 Cleaning PCR @18:18:00.70 AFV.select :carrierSolution @18:18:00.81 delay 2 seconds @18:18:02.84 AS.reconfigure ASconfig @18:18:02.89 AS.seek 0.5ml @18:18:13.16 delay 1 second @18:18:14.17 AFV.select :holdingCoil @18:18:14.28 ASV.dial :waste1 @18:18:14.65 ATV.dial :air3 @18:18:15.86 AS.reconfigure ASslow1 @18:18:15.91 AS.seek 0.51ml @18:18:18.13 delay 2 seconds @18:18:20.14 ATV.dial :bleach @18:18:20.74 AS.seek 0.81ml @18:19:21.32 delay 3 seconds @18:19:24.34 ATV.dial :air3 @18:19:24.87 delay 3 seconds @18:19:27.96 AS.seek 0.835ml @18:19:33.26 delay 3 seconds @18:19:36.28 ATV.dial :bleach @18:19:36.88 AS.seek 0.82ml @18:19:40.17 delay 3 seconds @18:19:43.19 ARV.dial :waste @18:19:43.78 delay 3 seconds @18:19:46.80 ATV.dial :ARV @18:19:47.66 AS.reconfigure ASconfig @18:19:47.71 AS.seek 0.47ml @18:20:08.12 AFV.select :carrierSolution @18:20:08.24 delay 2 seconds @18:20:10.27 AS.seek 1ml @18:20:41.03 delay 1 second @18:20:42.04 AFV.select :holdingCoil @18:20:42.15 delay 1 second @18:20:43.18 AS.reconfigure ASfast @18:20:43.23 AS.seek 0ml @18:21:10.55 AFV.select :carrierSolution @18:21:10.67 delay 2 seconds @18:21:12.70 AS.reconfigure ASconfig @18:21:12.75 AS.seek 1ml @18:22:10.53 delay 1 second @18:22:11.54 AFV.select :holdingCoil @18:22:11.65 delay 1 second @18:22:12.68 AS.reconfigure ASfast @18:22:12.73 AS.seek 0ml @18:22:40.06 AFV.select :carrierSolution @18:22:40.18 delay 2 seconds @18:22:42.21 AS.reconfigure ASconfig @18:22:42.26 AS.seek 1ml @18:23:40.04 delay 1 second @18:23:41.05 AFV.select :holdingCoil @18:23:41.16 delay 1 second @18:23:42.19 AS.reconfigure ASfast @18:23:42.24 AS.seek 0ml @18:24:10.16 ARV.dial between carrier and air1 @18:24:10.65 ATV.dial :air3 @18:24:11.86 AS.reconfigure ASslow1 @18:24:11.91 AS.seek 0.01ml @18:24:14.31 delay 2 seconds @18:24:16.32 ATV.dial :bleach @18:24:16.92 AS.seek 0.81ml @18:26:58.09 delay 3 seconds @18:27:01.11 ATV.dial :air3 @18:27:01.64 delay 3 seconds @18:27:04.73 AS.seek 0.835ml @18:27:10.03 delay 3 seconds @18:27:13.05 ATV.dial :bleach @18:27:13.66 AS.seek 0.82ml @18:27:16.98 delay 3 seconds @18:27:20.00 ATV.dial PCR @18:27:20.39 AS.reconfigure ASconfig @18:27:20.44 AS.seek :empty @18:28:07.89 PCR.program :bleachProfile @18:28:07.95 PCR program should complete in < 2:45 @18:28:07.96 PCR.run 10:00 @18:31:18.87 PCR.off @18:31:18.90 AFV.select :carrierSolution @18:31:19.01 delay 2 seconds @18:31:21.04 AS.seek 1ml @18:32:18.84 delay 1 second @18:32:19.85 AFV.select :holdingCoil @18:32:19.96 delay 1 second @18:32:20.99 AS.reconfigure ASfast @18:32:21.04 AS.seek 0ml @18:32:48.39 delay 3 seconds @18:32:51.41 AFV.select :carrierSolution @18:32:51.52 delay 2 seconds @18:32:53.55 AS.reconfigure ASconfig @18:32:53.61 AS.seek 1ml @18:33:51.42 delay 1 second @18:33:52.43 AFV.select :holdingCoil @18:33:52.54 delay 1 second @18:33:53.57 AS.reconfigure ASfast @18:33:53.62 AS.seek 0ml @18:34:20.97 delay 3 seconds @18:34:23.99 AFV.select :carrierSolution @18:34:24.10 delay 2 seconds @18:34:26.13 AS.reconfigure ASconfig @18:34:26.18 AS.seek 1ml @18:35:23.97 delay 1 second @18:35:24.98 AFV.select :holdingCoil @18:35:25.09 delay 1 second @18:35:26.12 AS.reconfigure ASfast @18:35:26.17 AS.seek 0ml @18:35:53.51 delay 3 seconds @18:35:56.53 AFV.select :carrierSolution @18:35:56.64 delay 2 seconds @18:35:58.67 AS.reconfigure ASconfig @18:35:58.73 AS.seek 1ml @18:36:56.51 delay 1 second @18:36:57.52 AFV.select :holdingCoil @18:36:57.63 delay 1 second @18:36:58.66 AS.reconfigure ASfast @18:36:58.71 AS.seek 0ml @18:37:26.05 delay 3 seconds @18:37:29.07 AFV.select :carrierSolution @18:37:29.18 delay 2 seconds @18:37:31.21 AS.reconfigure ASconfig @18:37:31.27 AS.seek 1ml @18:38:29.06 delay 1 second @18:38:30.07 AFV.select :holdingCoil @18:38:30.18 delay 1 second @18:38:31.21 AS.reconfigure ASnorm @18:38:31.26 AS.seek 0ml @18:39:21.97 Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0718h1500ml.pcr @18:39:21.99 Building PCR Slug from ASV with MM2 mix, PP3 primer @18:39:22.00 Priming with carrier @18:39:22.01 ATV.dial ARV @18:39:22.58 ARV.dial :carrier @18:39:22.93 AS.seek 0.5ml @18:39:48.47 AFV.select :carrierSolution @18:39:48.59 delay 2 seconds @18:39:50.62 AS.reconfigure ASconfig @18:39:50.67 AS.seek 1ml @18:40:19.70 delay 1 second @18:40:20.72 AFV.select :holdingCoil @18:40:20.83 delay 1 second @18:40:21.85 ATV.dial :waste @18:40:23.48 AS.reconfigure ASnorm @18:40:23.53 AS.seek :empty @18:41:14.30 AFV.select :carrierSolution @18:41:14.42 delay 2 seconds @18:41:16.45 AS.reconfigure ASconfig @18:41:16.50 AS.seek 0.5ml @18:41:45.59 delay 1 second @18:41:46.60 AFV.select :holdingCoil @18:41:46.71 Building Slug @18:41:46.72 ATV.dial :ARV @18:41:48.31 delay 2 seconds @18:41:50.91 ARV.dial :air1 @18:41:51.31 delay 2 seconds @18:41:53.40 AS.reconfigure ASslow2 @18:41:53.45 AS.seek 0.506ml @18:41:59.67 delay 2 seconds @18:42:01.69 ARV.dial :MM2 @18:42:03.16 delay 2 seconds @18:42:05.19 Adding MM2 @18:42:05.26 AS.seek 0.524ml @18:42:23.83 delay 25 seconds @18:42:48.85 ARV.dial :PP3 @18:42:49.24 Adding PP3 @18:42:49.26 delay 2 seconds @18:42:51.34 AS.seek 0.53ml @18:42:57.84 delay 10 seconds @18:43:07.86 ARV.dial :air2 @18:43:08.24 adding ARV air @18:43:08.26 delay 2 seconds @18:43:10.34 AS.seek 0.536ml @18:43:16.67 delay 5 seconds @18:43:21.69 ARV.dial :carrier @18:43:22.89 delay 7 seconds @18:43:29.98 AS.seek 0.594ml @18:44:28.83 delay 5 seconds @18:44:33.85 ATV.dial :ASV @18:44:34.25 delay 2 seconds @18:44:36.34 AS.seek 0.6ml @18:44:42.67 delay 4 seconds @18:44:46.69 ATV.dial ARV @18:44:47.22 delay 2 seconds @18:44:49.25 ARV.dial :carrier @18:44:49.26 delay 2 seconds @18:44:51.35 AS.seek 0.65ml @18:45:41.78 delay 4 seconds @18:45:45.80 ATV.dial PCR @18:45:46.38 ASV.dial :waste1 @18:45:46.39 ARV.dial :air1 @18:45:46.79 Positioning Slug by 0.324ml @18:45:46.88 AS.seek 0.326ml @18:51:12.94 delay 5 seconds @18:51:17.97 ATV.dial ARV @18:51:18.68 ASV.dial :PCRlock @18:51:19.05 delay 5 seconds @18:51:24.08 Running Rp Profile60C300 thermocycling @18:51:24.09 PCR.program Profile60C300 @18:51:24.23 Profile60C300 should complete in < 1:45:21 @18:51:24.24 PCR.run 2:16:41.25 @20:13:26.52 PCR.off @20:13:28.09 PCR for Rp completed -- closed /var/log/esp/Rp18oct0718h1500ml.pcr @20:13:28.16 Cleaning PCR @20:13:28.17 AFV.select :carrierSolution @20:13:28.28 delay 2 seconds @20:13:30.31 AS.reconfigure ASconfig @20:13:30.36 AS.seek 0.5ml @20:13:40.69 delay 1 second @20:13:41.70 AFV.select :holdingCoil @20:13:41.81 ASV.dial :waste1 @20:13:42.17 ATV.dial :air3 @20:13:43.38 AS.reconfigure ASslow1 @20:13:43.43 AS.seek 0.51ml @20:13:45.72 delay 2 seconds @20:13:47.74 ATV.dial :bleach @20:13:48.34 AS.seek 0.81ml @20:14:49.00 delay 3 seconds @20:14:52.02 ATV.dial :air3 @20:14:52.55 delay 3 seconds @20:14:55.64 AS.seek 0.835ml @20:15:00.97 delay 3 seconds @20:15:04.00 ATV.dial :bleach @20:15:04.59 AS.seek 0.82ml @20:15:07.89 delay 3 seconds @20:15:10.91 ARV.dial :waste @20:15:11.50 delay 3 seconds @20:15:14.52 ATV.dial :ARV @20:15:15.36 AS.reconfigure ASconfig @20:15:15.41 AS.seek 0.47ml @20:15:35.83 AFV.select :carrierSolution @20:15:35.95 delay 2 seconds @20:15:37.98 AS.seek 1ml @20:16:08.73 delay 1 second @20:16:09.75 AFV.select :holdingCoil @20:16:09.86 delay 1 second @20:16:10.89 AS.reconfigure ASfast @20:16:10.94 AS.seek 0ml @20:16:38.27 AFV.select :carrierSolution @20:16:38.39 delay 2 seconds @20:16:40.42 AS.reconfigure ASconfig @20:16:40.47 AS.seek 1ml @20:17:38.25 delay 1 second @20:17:39.83 AFV.select :holdingCoil @20:17:39.97 delay 1 second @20:17:41.00 AS.reconfigure ASfast @20:17:41.05 AS.seek 0ml @20:18:08.38 AFV.select :carrierSolution @20:18:08.50 delay 2 seconds @20:18:10.53 AS.reconfigure ASconfig @20:18:10.58 AS.seek 1ml @20:19:08.38 delay 1 second @20:19:09.39 AFV.select :holdingCoil @20:19:09.50 delay 1 second @20:19:10.53 AS.reconfigure ASfast @20:19:10.58 AS.seek 0ml @20:19:37.94 ARV.dial between carrier and air1 @20:19:38.42 ATV.dial :air3 @20:19:39.63 AS.reconfigure ASslow1 @20:19:39.68 AS.seek 0.01ml @20:19:42.11 delay 2 seconds @20:19:44.12 ATV.dial :bleach @20:19:44.73 AS.seek 0.81ml @20:22:25.91 delay 3 seconds @20:22:28.93 ATV.dial :air3 @20:22:29.46 delay 3 seconds @20:22:32.55 AS.seek 0.835ml @20:22:37.87 delay 3 seconds @20:22:40.89 ATV.dial :bleach @20:22:41.49 AS.seek 0.82ml @20:22:44.77 delay 3 seconds @20:22:47.79 ATV.dial PCR @20:22:48.17 AS.reconfigure ASconfig @20:22:48.22 AS.seek :empty @20:23:35.68 PCR.program :bleachProfile @20:23:35.75 PCR program should complete in < 2:45 @20:23:35.76 PCR.run 10:00 @20:26:43.65 PCR.off @20:26:43.67 AFV.select :carrierSolution @20:26:43.78 delay 2 seconds @20:26:45.82 AS.seek 1ml @20:27:43.68 delay 1 second @20:27:44.69 AFV.select :holdingCoil @20:27:44.80 delay 1 second @20:27:45.83 AS.reconfigure ASfast @20:27:45.88 AS.seek 0ml @20:28:13.22 delay 3 seconds @20:28:16.24 AFV.select :carrierSolution @20:28:16.35 delay 2 seconds @20:28:18.38 AS.reconfigure ASconfig @20:28:18.44 AS.seek 1ml @20:29:16.22 delay 1 second @20:29:17.23 AFV.select :holdingCoil @20:29:17.34 delay 1 second @20:29:18.37 AS.reconfigure ASfast @20:29:18.42 AS.seek 0ml @20:29:45.77 delay 3 seconds @20:29:48.79 AFV.select :carrierSolution @20:29:48.90 delay 2 seconds @20:29:50.93 AS.reconfigure ASconfig @20:29:50.98 AS.seek 1ml @20:30:48.77 delay 1 second @20:30:49.78 AFV.select :holdingCoil @20:30:49.89 delay 1 second @20:30:50.92 AS.reconfigure ASfast @20:30:50.97 AS.seek 0ml @20:31:18.32 delay 3 seconds @20:31:21.34 AFV.select :carrierSolution @20:31:21.45 delay 2 seconds @20:31:23.48 AS.reconfigure ASconfig @20:31:23.54 AS.seek 1ml @20:32:21.33 delay 1 second @20:32:22.34 AFV.select :holdingCoil @20:32:22.45 delay 1 second @20:32:23.48 AS.reconfigure ASfast @20:32:23.53 AS.seek 0ml @20:32:50.86 delay 3 seconds @20:32:53.88 AFV.select :carrierSolution @20:32:53.99 delay 2 seconds @20:32:56.02 AS.reconfigure ASconfig @20:32:56.07 AS.seek 1ml @20:33:53.87 delay 1 second @20:33:54.88 AFV.select :holdingCoil @20:33:54.99 delay 1 second @20:33:56.02 AS.reconfigure ASnorm @20:33:56.07 AS.seek 0ml @20:34:46.77 Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0720h1500ml.pcr @20:34:46.79 Building PCR Slug from ASV with MM3 mix, PP5 primer @20:34:46.81 Priming with carrier @20:34:46.82 ATV.dial ARV @20:34:47.40 ARV.dial :carrier @20:34:47.75 AS.seek 0.5ml @20:35:13.26 AFV.select :carrierSolution @20:35:13.38 delay 2 seconds @20:35:15.41 AS.reconfigure ASconfig @20:35:15.46 AS.seek 1ml @20:35:44.48 delay 1 second @20:35:45.49 AFV.select :holdingCoil @20:35:45.60 delay 1 second @20:35:46.62 ATV.dial :waste @20:35:48.15 AS.reconfigure ASnorm @20:35:48.80 AS.seek :empty @20:36:39.56 AFV.select :carrierSolution @20:36:39.68 delay 2 seconds @20:36:41.71 AS.reconfigure ASconfig @20:36:41.76 AS.seek 0.5ml @20:37:10.83 delay 1 second @20:37:11.85 AFV.select :holdingCoil @20:37:11.96 Building Slug @20:37:11.97 ATV.dial :ARV @20:37:13.57 delay 2 seconds @20:37:15.59 ARV.dial :air1 @20:37:16.09 delay 2 seconds @20:37:18.18 AS.reconfigure ASslow2 @20:37:18.23 AS.seek 0.506ml @20:37:24.57 delay 2 seconds @20:37:26.58 ARV.dial :MM3 @20:37:27.57 delay 2 seconds @20:37:29.60 Adding MM3 @20:37:29.68 AS.seek 0.524ml @20:37:48.26 delay 25 seconds @20:38:13.28 ARV.dial :PP5 @20:38:13.66 Adding PP5 @20:38:13.67 delay 2 seconds @20:38:15.76 AS.seek 0.53ml @20:38:22.19 delay 10 seconds @20:38:32.21 ARV.dial :air3 @20:38:32.59 adding ARV air @20:38:32.60 delay 2 seconds @20:38:34.69 AS.seek 0.536ml @20:38:41.04 delay 5 seconds @20:38:46.06 ARV.dial :carrier @20:38:47.26 delay 7 seconds @20:38:54.35 AS.seek 0.594ml @20:39:52.93 delay 5 seconds @20:39:57.96 ATV.dial :ASV @20:39:58.35 delay 2 seconds @20:40:00.44 AS.seek 0.6ml @20:40:06.66 delay 4 seconds @20:40:10.68 ATV.dial ARV @20:40:11.30 delay 2 seconds @20:40:13.32 ARV.dial :carrier @20:40:13.34 delay 2 seconds @20:40:15.43 AS.seek 0.65ml @20:41:05.95 delay 4 seconds @20:41:09.98 ATV.dial PCR @20:41:10.57 ASV.dial :waste1 @20:41:10.58 ARV.dial :air1 @20:41:10.96 Positioning Slug by 0.324ml @20:41:11.05 AS.seek 0.326ml @20:46:37.17 delay 5 seconds @20:46:42.19 ATV.dial ARV @20:46:43.03 ASV.dial :PCRlock @20:46:43.40 delay 5 seconds @20:46:48.43 Running Bb Profile60C300 thermocycling @20:46:48.45 PCR.program Profile60C300 @20:46:48.58 Profile60C300 should complete in < 1:45:21 @20:46:48.59 PCR.run 2:16:41.25 @22:08:42.05 PCR.off @22:08:43.62 PCR for Bb completed -- closed /var/log/esp/Bb18oct0720h1500ml.pcr @22:08:43.69 Cleaning PCR @22:08:43.70 AFV.select :carrierSolution @22:08:43.81 delay 2 seconds @22:08:45.84 AS.reconfigure ASconfig @22:08:45.89 AS.seek 0.5ml @22:08:56.19 delay 1 second @22:08:57.20 AFV.select :holdingCoil @22:08:57.31 ASV.dial :waste1 @22:08:57.68 ATV.dial :air3 @22:08:58.89 AS.reconfigure ASslow1 @22:08:58.94 AS.seek 0.51ml @22:09:01.24 delay 2 seconds @22:09:03.26 ATV.dial :bleach @22:09:03.86 AS.seek 0.81ml @22:10:04.46 delay 3 seconds @22:10:07.48 ATV.dial :air3 @22:10:08.01 delay 3 seconds @22:10:11.10 AS.seek 0.835ml @22:10:16.39 delay 3 seconds @22:10:19.41 ATV.dial :bleach @22:10:20.01 AS.seek 0.82ml @22:10:23.32 delay 3 seconds @22:10:26.34 ARV.dial :waste @22:10:26.91 delay 3 seconds @22:10:29.93 ATV.dial :ARV @22:10:30.77 AS.reconfigure ASconfig @22:10:30.83 AS.seek 0.47ml @22:10:51.23 AFV.select :carrierSolution @22:10:51.35 delay 2 seconds @22:10:53.38 AS.seek 1ml @22:11:24.74 delay 1 second @22:11:25.75 AFV.select :holdingCoil @22:11:25.86 delay 1 second @22:11:26.89 AS.reconfigure ASfast @22:11:26.94 AS.seek 0ml @22:11:54.28 AFV.select :carrierSolution @22:11:54.40 delay 2 seconds @22:11:56.43 AS.reconfigure ASconfig @22:11:56.48 AS.seek 1ml @22:12:54.27 delay 1 second @22:12:55.28 AFV.select :holdingCoil @22:12:55.39 delay 1 second @22:12:56.42 AS.reconfigure ASfast @22:12:56.47 AS.seek 0ml @22:13:23.79 AFV.select :carrierSolution @22:13:23.91 delay 2 seconds @22:13:25.94 AS.reconfigure ASconfig @22:13:25.99 AS.seek 1ml @22:14:23.77 delay 1 second @22:14:24.78 AFV.select :holdingCoil @22:14:24.89 delay 1 second @22:14:25.92 AS.reconfigure ASfast @22:14:25.97 AS.seek 0ml @22:14:53.34 ARV.dial between carrier and air1 @22:14:53.82 ATV.dial :air3 @22:14:55.03 AS.reconfigure ASslow1 @22:14:55.09 AS.seek 0.01ml @22:14:57.49 delay 2 seconds @22:14:59.50 ATV.dial :bleach @22:15:00.12 AS.seek 0.81ml @22:17:41.38 delay 3 seconds @22:17:44.40 ATV.dial :air3 @22:17:44.93 delay 3 seconds @22:17:48.02 AS.seek 0.835ml @22:17:53.35 delay 3 seconds @22:17:56.37 ATV.dial :bleach @22:17:56.97 AS.seek 0.82ml @22:18:00.27 delay 3 seconds @22:18:03.29 ATV.dial PCR @22:18:03.68 AS.reconfigure ASconfig @22:18:03.73 AS.seek :empty @22:18:51.21 PCR.program :bleachProfile @22:18:51.27 PCR program should complete in < 2:45 @22:18:51.28 PCR.run 10:00 @22:21:59.16 PCR.off @22:21:59.18 AFV.select :carrierSolution @22:21:59.30 delay 2 seconds @22:22:01.33 AS.seek 1ml @22:22:59.13 delay 1 second @22:23:00.14 AFV.select :holdingCoil @22:23:00.25 delay 1 second @22:23:01.28 AS.reconfigure ASfast @22:23:01.33 AS.seek 0ml @22:23:28.67 delay 3 seconds @22:23:31.69 AFV.select :carrierSolution @22:23:31.80 delay 2 seconds @22:23:33.83 AS.reconfigure ASconfig @22:23:33.89 AS.seek 1ml @22:24:31.68 delay 1 second @22:24:32.70 AFV.select :holdingCoil @22:24:32.81 delay 1 second @22:24:33.84 AS.reconfigure ASfast @22:24:33.89 AS.seek 0ml @22:25:01.25 delay 3 seconds @22:25:04.27 AFV.select :carrierSolution @22:25:04.38 delay 2 seconds @22:25:06.41 AS.reconfigure ASconfig @22:25:06.46 AS.seek 1ml @22:26:04.25 delay 1 second @22:26:05.26 AFV.select :holdingCoil @22:26:05.37 delay 1 second @22:26:06.40 AS.reconfigure ASfast @22:26:06.45 AS.seek 0ml @22:26:33.79 delay 3 seconds @22:26:36.81 AFV.select :carrierSolution @22:26:36.92 delay 2 seconds @22:26:38.96 AS.reconfigure ASconfig @22:26:39.00 AS.seek 1ml @22:27:36.79 delay 1 second @22:27:37.80 AFV.select :holdingCoil @22:27:37.91 delay 1 second @22:27:38.94 AS.reconfigure ASfast @22:27:38.99 AS.seek 0ml @22:28:06.32 delay 3 seconds @22:28:09.34 AFV.select :carrierSolution @22:28:09.45 delay 2 seconds @22:28:11.48 AS.reconfigure ASconfig @22:28:11.53 AS.seek 1ml @22:29:09.31 delay 1 second @22:29:10.32 AFV.select :holdingCoil @22:29:10.43 delay 1 second @22:29:11.46 AS.reconfigure ASnorm @22:29:11.51 AS.seek 0ml @22:30:02.21 Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0722h1500ml.pcr @22:30:02.23 Building PCR Slug from ASV with MM1 mix, PP2 primer @22:30:02.25 Priming with carrier @22:30:02.26 ATV.dial ARV @22:30:02.93 ARV.dial :carrier @22:30:03.29 AS.seek 0.5ml @22:30:29.52 AFV.select :carrierSolution @22:30:29.64 delay 2 seconds @22:30:31.67 AS.reconfigure ASconfig @22:30:31.72 AS.seek 1ml @22:31:00.76 delay 1 second @22:31:01.78 AFV.select :holdingCoil @22:31:01.89 delay 1 second @22:31:02.91 ATV.dial :waste @22:31:04.45 AS.reconfigure ASnorm @22:31:04.50 AS.seek :empty @22:31:55.22 AFV.select :carrierSolution @22:31:55.34 delay 2 seconds @22:31:57.37 AS.reconfigure ASconfig @22:31:57.42 AS.seek 0.5ml @22:32:26.48 delay 1 second @22:32:27.49 AFV.select :holdingCoil @22:32:27.60 Building Slug @22:32:27.61 ATV.dial :ARV @22:32:29.22 delay 2 seconds @22:32:31.24 ARV.dial :air1 @22:32:31.65 delay 2 seconds @22:32:33.73 AS.reconfigure ASslow2 @22:32:33.79 AS.seek 0.506ml @22:32:40.15 delay 2 seconds @22:32:42.17 ARV.dial :MM1 @22:32:42.83 delay 2 seconds @22:32:44.86 Adding MM1 @22:32:44.93 AS.seek 0.524ml @22:33:03.47 delay 25 seconds @22:33:28.49 ARV.dial :PP2 @22:33:28.87 Adding PP2 @22:33:28.88 delay 2 seconds @22:33:30.97 AS.seek 0.53ml @22:33:37.20 delay 10 seconds @22:33:47.22 ARV.dial :air2 @22:33:47.69 adding ARV air @22:33:47.70 delay 2 seconds @22:33:49.79 AS.seek 0.536ml @22:33:56.26 delay 5 seconds @22:34:01.28 ARV.dial :carrier @22:34:02.48 delay 7 seconds @22:34:09.57 AS.seek 0.594ml @22:35:08.08 delay 5 seconds @22:35:13.10 ATV.dial :ASV @22:35:13.50 delay 2 seconds @22:35:15.59 AS.seek 0.6ml @22:35:21.76 delay 4 seconds @22:35:25.78 ATV.dial ARV @22:35:26.40 delay 2 seconds @22:35:28.42 ARV.dial :carrier @22:35:28.44 delay 2 seconds @22:35:30.53 AS.seek 0.65ml @22:36:21.06 delay 4 seconds @22:36:25.08 ATV.dial PCR @22:36:25.76 ASV.dial :waste1 @22:36:25.77 ARV.dial :air1 @22:36:26.16 Positioning Slug by 0.324ml @22:36:26.25 AS.seek 0.326ml @22:41:52.33 delay 5 seconds @22:41:57.35 ATV.dial ARV @22:41:58.08 ASV.dial :PCRlock @22:41:58.45 delay 5 seconds @22:42:03.48 Running Ss Profile60C350 thermocycling @22:42:03.49 PCR.program Profile60C350 @22:42:03.63 Profile60C350 should complete in < 1:48:05 @22:42:03.64 PCR.run 2:20:06.25 @00:04:28.72CEST08-Oct-18 PCR.off @00:04:30.78 PCR for Ss completed -- closed /var/log/esp/Ss18oct0722h1500ml.pcr @00:04:30.86 Cleaning PCR @00:04:30.87 AFV.select :carrierSolution @00:04:30.98 delay 2 seconds @00:04:33.01 AS.reconfigure ASconfig @00:04:33.06 AS.seek 0.5ml @00:04:43.37 delay 1 second @00:04:44.38 AFV.select :holdingCoil @00:04:44.49 ASV.dial :waste1 @00:04:44.85 ATV.dial :air3 @00:04:46.07 AS.reconfigure ASslow1 @00:04:46.12 AS.seek 0.51ml @00:04:48.43 delay 2 seconds @00:04:51.04 ATV.dial :bleach @00:04:51.64 AS.seek 0.81ml @00:05:52.21 delay 3 seconds @00:05:55.23 ATV.dial :air3 @00:05:55.76 delay 3 seconds @00:05:58.85 AS.seek 0.835ml @00:06:04.21 delay 3 seconds @00:06:07.23 ATV.dial :bleach @00:06:07.83 AS.seek 0.82ml @00:06:11.12 delay 3 seconds @00:06:14.14 ARV.dial :waste @00:06:14.73 delay 3 seconds @00:06:17.75 ATV.dial :ARV @00:06:18.60 AS.reconfigure ASconfig @00:06:18.65 AS.seek 0.47ml @00:06:39.05 AFV.select :carrierSolution @00:06:39.17 delay 2 seconds @00:06:41.20 AS.seek 1ml @00:07:11.98 delay 1 second @00:07:13.00 AFV.select :holdingCoil @00:07:13.11 delay 1 second @00:07:14.14 AS.reconfigure ASfast @00:07:14.19 AS.seek 0ml @00:07:41.52 AFV.select :carrierSolution @00:07:41.64 delay 2 seconds @00:07:43.67 AS.reconfigure ASconfig @00:07:43.72 AS.seek 1ml @00:08:41.50 delay 1 second @00:08:42.51 AFV.select :holdingCoil @00:08:42.62 delay 1 second @00:08:43.65 AS.reconfigure ASfast @00:08:43.70 AS.seek 0ml @00:09:11.03 AFV.select :carrierSolution @00:09:11.15 delay 2 seconds @00:09:13.18 AS.reconfigure ASconfig @00:09:13.23 AS.seek 1ml @00:10:11.04 delay 1 second @00:10:12.05 AFV.select :holdingCoil @00:10:12.16 delay 1 second @00:10:13.19 AS.reconfigure ASfast @00:10:13.24 AS.seek 0ml @00:10:40.57 ARV.dial between carrier and air1 @00:10:41.06 ATV.dial :air3 @00:10:42.27 AS.reconfigure ASslow1 @00:10:42.32 AS.seek 0.01ml @00:10:44.67 delay 2 seconds @00:10:46.69 ATV.dial :bleach @00:10:47.29 AS.seek 0.81ml @00:13:28.42 delay 3 seconds @00:13:31.44 ATV.dial :air3 @00:13:31.97 delay 3 seconds @00:13:35.06 AS.seek 0.835ml @00:13:40.38 delay 3 seconds @00:13:43.40 ATV.dial :bleach @00:13:44.01 AS.seek 0.82ml @00:13:47.34 delay 3 seconds @00:13:50.36 ATV.dial PCR @00:13:50.75 AS.reconfigure ASconfig @00:13:50.80 AS.seek :empty @00:14:38.25 PCR.program :bleachProfile @00:14:38.32 PCR program should complete in < 2:45 @00:14:38.33 PCR.run 10:00 @00:17:46.21 PCR.off @00:17:46.24 AFV.select :carrierSolution @00:17:46.35 delay 2 seconds @00:17:48.38 AS.seek 1ml @00:18:46.18 delay 1 second @00:18:47.20 AFV.select :holdingCoil @00:18:47.31 delay 1 second @00:18:48.34 AS.reconfigure ASfast @00:18:48.39 AS.seek 0ml @00:19:15.73 delay 3 seconds @00:19:18.75 AFV.select :carrierSolution @00:19:18.86 delay 2 seconds @00:19:20.89 AS.reconfigure ASconfig @00:19:20.94 AS.seek 1ml @00:20:18.72 delay 1 second @00:20:19.73 AFV.select :holdingCoil @00:20:19.84 delay 1 second @00:20:20.87 AS.reconfigure ASfast @00:20:20.92 AS.seek 0ml @00:20:48.25 delay 3 seconds @00:20:51.27 AFV.select :carrierSolution @00:20:51.38 delay 2 seconds @00:20:53.41 AS.reconfigure ASconfig @00:20:53.47 AS.seek 1ml @00:21:51.25 delay 1 second @00:21:52.26 AFV.select :holdingCoil @00:21:52.37 delay 1 second @00:21:53.40 AS.reconfigure ASfast @00:21:53.45 AS.seek 0ml @00:22:20.88 delay 3 seconds @00:22:23.90 AFV.select :carrierSolution @00:22:24.01 delay 2 seconds @00:22:26.04 AS.reconfigure ASconfig @00:22:26.09 AS.seek 1ml @00:23:23.84 delay 1 second @00:23:24.85 AFV.select :holdingCoil @00:23:24.96 delay 1 second @00:23:25.99 AS.reconfigure ASfast @00:23:26.04 AS.seek 0ml @00:23:53.39 delay 3 seconds @00:23:56.41 AFV.select :carrierSolution @00:23:56.52 delay 2 seconds @00:23:58.55 AS.reconfigure ASconfig @00:23:58.62 AS.seek 1ml @00:24:56.41 delay 1 second @00:24:57.42 AFV.select :holdingCoil @00:24:57.53 delay 1 second @00:24:58.56 AS.reconfigure ASnorm @00:24:58.61 AS.seek 0ml @00:25:49.32 Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0800h1500ml.pcr @00:25:49.34 Building PCR Slug from ASV with MM1 mix, PP1 primer @00:25:49.35 Priming with carrier @00:25:49.36 ATV.dial ARV @00:25:50.05 ARV.dial :carrier @00:25:50.40 AS.seek 0.5ml @00:26:16.02 AFV.select :carrierSolution @00:26:16.14 delay 2 seconds @00:26:18.17 AS.reconfigure ASconfig @00:26:18.22 AS.seek 1ml @00:26:47.24 delay 1 second @00:26:48.26 AFV.select :holdingCoil @00:26:48.37 delay 1 second @00:26:49.39 ATV.dial :waste @00:26:50.92 AS.reconfigure ASnorm @00:26:50.97 AS.seek :empty @00:27:41.68 AFV.select :carrierSolution @00:27:41.80 delay 2 seconds @00:27:43.83 AS.reconfigure ASconfig @00:27:43.88 AS.seek 0.5ml @00:28:12.99 delay 1 second @00:28:14.00 AFV.select :holdingCoil @00:28:14.11 Building Slug @00:28:14.12 ATV.dial :ARV @00:28:15.63 delay 2 seconds @00:28:17.65 ARV.dial :air1 @00:28:18.17 delay 2 seconds @00:28:20.26 AS.reconfigure ASslow2 @00:28:20.31 AS.seek 0.506ml @00:28:26.58 delay 2 seconds @00:28:28.60 ARV.dial :MM1 @00:28:29.17 delay 2 seconds @00:28:31.20 Adding MM1 @00:28:31.27 AS.seek 0.524ml @00:28:49.61 delay 25 seconds @00:29:14.63 ARV.dial :PP1 @00:29:15.00 Adding PP1 @00:29:15.01 delay 2 seconds @00:29:17.10 AS.seek 0.53ml @00:29:23.46 delay 10 seconds @00:29:33.48 ARV.dial :air1 @00:29:33.88 adding ARV air @00:29:33.89 delay 2 seconds @00:29:35.99 AS.seek 0.536ml @00:29:42.35 delay 5 seconds @00:29:47.37 ARV.dial :carrier @00:29:47.75 delay 7 seconds @00:29:54.84 AS.seek 0.594ml @00:30:53.57 delay 5 seconds @00:30:58.59 ATV.dial :ASV @00:30:58.97 delay 2 seconds @00:31:01.06 AS.seek 0.6ml @00:31:07.43 delay 4 seconds @00:31:11.45 ATV.dial ARV @00:31:11.97 delay 2 seconds @00:31:13.99 ARV.dial :carrier @00:31:14.01 delay 2 seconds @00:31:16.10 AS.seek 0.65ml @00:32:06.93 delay 4 seconds @00:32:10.95 ATV.dial PCR @00:32:11.55 ASV.dial :waste1 @00:32:11.56 ARV.dial :air1 @00:32:11.95 Positioning Slug by 0.324ml @00:32:12.04 AS.seek 0.326ml @00:37:38.24 delay 5 seconds @00:37:43.26 ATV.dial ARV @00:37:44.01 ASV.dial :PCRlock @00:37:44.38 delay 5 seconds @00:37:49.41 Running IPc ProfileIPc thermocycling @00:37:49.42 PCR.program ProfileIPc @00:37:49.58 ProfileIPc should complete in < 1:50:49 @00:37:49.59 PCR.run 2:23:31.25 @02:01:56.75 PCR.off @02:01:59.39 PCR for IPc completed -- closed /var/log/esp/IPc18oct0800h1500ml.pcr @02:01:59.41 Full MFB Decontamination @02:01:59.42 Decon extraction column and decon AIV @02:01:59.44 SPE.hold 2:46:40,55C @02:01:59.48 ASV.dial :SPEdelivery @02:02:00.08 delay 20 seconds @02:02:20.11 AFV.select :carrierSolution @02:02:20.22 delay 2 seconds @02:02:22.25 AS.reconfigure ASconfig @02:02:22.30 AS.seek 0.5ml @02:02:32.59 delay 1 second @02:02:33.60 AFV.select :holdingCoil @02:02:33.71 ATV.dial :air1 @02:02:35.43 AS.reconfigure ASslow1 @02:02:35.48 AS.seek 0.52ml @02:02:39.77 ATV.dial :ASV @02:02:41.38 ASV.dial :SPErecovery @02:02:42.02 AS.seek 0.62ml @02:03:02.38 ATV.dial :waste @02:03:04.19 AS.reconfigure ASnorm @02:03:04.24 AS.seek :empty @02:03:35.85 AFV.select :holdingCoil @02:03:35.87 ATV.dial :waste @02:03:35.89 AS.reconfigure ASconfig @02:03:35.94 AS.seek 0ml @02:03:36.22 AFV.select :carrierSolution @02:03:36.34 delay 2 seconds @02:03:38.37 AS.seek 0.5ml @02:04:07.41 delay 1 second @02:04:08.42 AFV.select :holdingCoil @02:04:08.53 ATV.dial :air3 @02:04:09.20 AS.reconfigure ASslow1 @02:04:09.25 AS.seek 0.52ml @02:04:13.54 ATV.dial :air3 @02:04:13.63 AS.seek 0.53ml @02:04:15.92 delay 2 seconds @02:04:17.93 ATV.dial :bleach @02:04:18.41 AS.seek 0.71ml @02:04:54.89 delay 3 seconds @02:04:57.91 ATV.dial :air3 @02:04:58.45 delay 3 seconds @02:05:01.54 AS.seek 0.735ml @02:05:06.79 delay 3 seconds @02:05:09.81 ATV.dial :bleach @02:05:10.52 AS.seek 0.72ml @02:05:13.83 delay 3 seconds @02:05:16.86 ATV.dial ASV @02:05:17.83 ASV.dial :SPErecovery @02:05:17.92 AS.seek 0.365ml @02:06:29.59 ATV.dial :waste @02:06:31.19 AS.reconfigure ASnorm @02:06:31.24 AS.seek :empty @02:06:50.01 ATV.dial :air3 @02:06:50.67 AS.reconfigure ASslow1 @02:06:50.72 AS.seek 0.009ml @02:06:53.01 delay 2 seconds @02:06:55.03 ATV.dial :bleach @02:06:55.51 AS.seek 0.579ml @02:08:50.39 delay 3 seconds @02:08:53.41 ATV.dial :air3 @02:08:53.94 delay 3 seconds @02:08:57.03 AS.seek 0.604ml @02:09:02.34 delay 3 seconds @02:09:05.36 ATV.dial :bleach @02:09:05.96 AS.seek 0.589ml @02:09:09.27 delay 3 seconds @02:09:12.29 ATV.dial :ASV @02:09:13.25 ASV.dial :wasteR @02:09:13.64 AS.seek 0ml @02:11:12.41 delay 1:00 @02:12:12.43 ATV.dial ASV @02:12:13.02 AFV.select :carrierSolution @02:12:13.15 delay 2 seconds @02:12:15.18 AS.reconfigure ASconfig @02:12:15.23 AS.seek 1ml @02:13:12.99 delay 1 second @02:13:14.01 AFV.select :holdingCoil @02:13:14.12 delay 1 second @02:13:15.14 ASV.dial :wasteR @02:13:15.17 AS.reconfigure ASslow1 @02:13:15.21 AS.seek 0ml @02:16:36.66 delay 10 seconds @02:16:46.73 ATV.dial :air3 @02:16:48.32 AS.seek 0.01ml @02:16:50.64 delay 2 seconds @02:16:52.66 ATV.dial :bleach @02:16:53.26 AS.seek 0.58ml @02:18:48.15 delay 3 seconds @02:18:51.17 ATV.dial :air3 @02:18:51.71 delay 3 seconds @02:18:54.80 AS.seek 0.605ml @02:19:00.08 delay 3 seconds @02:19:03.10 ATV.dial :bleach @02:19:03.70 AS.seek 0.59ml @02:19:07.00 delay 3 seconds @02:19:10.03 ATV.dial ASV @02:19:10.99 ASV.dial :SPEdelivery @02:19:11.36 AS.seek 0ml @02:21:10.27 delay 1:00 @02:22:10.29 AFV.select :carrierSolution @02:22:10.40 delay 2 seconds @02:22:12.43 AS.reconfigure ASconfig @02:22:12.48 AS.seek 1ml @02:23:10.24 delay 1 second @02:23:11.26 AFV.select :holdingCoil @02:23:11.37 delay 1 second @02:23:12.39 ATV.dial ASV @02:23:12.41 ASV.dial :SPEdelivery @02:23:12.43 AS.reconfigure ASslow1 @02:23:12.47 AS.seek 0ml @02:26:33.85 delay 10 seconds @02:26:43.87 ATV.dial :air2 @02:26:44.65 AS.reconfigure ASslow0 @02:26:44.71 AS.seek 0.8ml @02:28:05.51 delay 3 seconds @02:28:08.53 ATV.dial :ASV @02:28:09.46 ASV.dial :SPEdelivery @02:28:09.48 AS.reconfigure ASslow1 @02:28:09.53 AS.seek 0.03ml @02:30:44.68 delay 20 seconds @02:31:04.71 ATV.dial :waste,via: :air3 @02:31:06.38 AS.reconfigure ASconfig @02:31:06.43 AS.seek :empty @02:31:08.48 SPE.coast @02:31:08.57 Removing Bleach from Mixing Coil @02:31:08.58 AFV.select :carrierSolution @02:31:08.69 delay 2 seconds @02:31:10.72 AS.seek 0.5ml @02:31:39.81 delay 1 second @02:31:40.82 AFV.select :holdingCoil @02:31:40.93 ATV.dial :ASV @02:31:42.52 ASV.dial :SPErecovery @02:31:43.14 AS.reconfigure ASslow1 @02:31:43.19 AS.seek 0.73ml @02:32:29.70 ASV.dial :wasteD @02:32:30.24 AS.reconfigure ASnorm @02:32:30.29 AS.seek 0ml @02:33:07.40 AFV.select :carrierSolution @02:33:07.52 delay 2 seconds @02:33:09.55 AS.reconfigure ASconfig @02:33:09.60 AS.seek 0.5ml @02:33:38.68 delay 1 second @02:33:39.69 AFV.select :holdingCoil @02:33:39.80 ATV.dial ASV @02:33:39.81 ASV.dial :SPErecovery @02:33:40.44 AS.reconfigure ASslow0 @02:33:40.49 AS.seek 0.3ml @02:34:00.92 ATV.dial :air2 @02:34:01.92 AS.reconfigure ASslow1 @02:34:01.97 AS.seek 0.32ml @02:34:06.25 ATV.dial ASV @02:34:07.34 AS.reconfigure ASslow0 @02:34:07.39 AS.seek 0.57ml @02:34:32.80 ASV.dial :wasteD @02:34:33.37 AS.reconfigure ASnorm @02:34:33.42 AS.seek 0ml @02:35:02.47 AFV.select :carrierSolution @02:35:02.59 delay 2 seconds @02:35:04.62 AS.reconfigure ASconfig @02:35:04.67 AS.seek 0.5ml @02:35:33.71 delay 1 second @02:35:34.72 AFV.select :holdingCoil @02:35:34.83 ATV.dial ASV @02:35:34.84 ASV.dial :SPErecovery @02:35:35.47 AS.reconfigure ASslow0 @02:35:35.52 AS.seek 0.3ml @02:35:56.56 ATV.dial :air2 @02:35:57.55 AS.reconfigure ASslow1 @02:35:57.60 AS.seek 0.32ml @02:36:01.94 ATV.dial ASV @02:36:03.02 AS.reconfigure ASslow0 @02:36:03.07 AS.seek 0.57ml @02:36:28.52 ASV.dial :wasteD @02:36:29.09 AS.reconfigure ASnorm @02:36:29.15 AS.seek 0ml @02:36:58.20 ATV.dial :air2 @02:36:59.10 AS.reconfigure ASslow0 @02:36:59.16 AS.seek 0.3ml @02:37:29.70 ATV.dial :ASV @02:37:30.71 ASV.dial :wasteD @02:37:30.73 AS.reconfigure ASconfig @02:37:30.78 AS.seek 0.05ml @02:37:45.42 ATV.dial :waste @02:37:47.13 AS.seek 0ml @02:37:50.28 ASV.dial :SPEdelivery @02:37:51.23 deconLysateINT @02:37:51.24 ATV.dial :air3 @02:37:51.91 AS.reconfigure ASslow1 @02:37:51.96 AS.seek 0.01ml @02:37:54.32 delay 2 seconds @02:37:56.34 ATV.dial :bleach @02:37:56.81 AS.seek 0.96ml @02:41:08.18 delay 3 seconds @02:41:11.20 ATV.dial :air3 @02:41:11.73 delay 3 seconds @02:41:14.83 AS.seek 0.985ml @02:41:20.17 delay 3 seconds @02:41:23.19 ATV.dial :bleach @02:41:23.78 AS.seek 0.97ml @02:41:27.12 delay 3 seconds @02:41:30.15 ATV.dial AIV @02:41:31.31 AIV.dial :coilToMFB @02:41:31.32 AWV.select :waste @02:41:31.34 AFV.select :carrierSolution @02:41:31.45 delay 2 seconds @02:41:33.48 AS.reconfigure ASconfig @02:41:33.53 AS.seek 1ml @02:41:35.54 delay 1 second @02:41:36.56 AFV.select :holdingCoil @02:41:36.67 delay 1 second @02:41:37.70 AS.reconfigure ASslow0 @02:41:37.74 AS.seek :empty @02:43:18.70 AFV.select :carrierSolution @02:43:18.82 delay 2 seconds @02:43:20.85 AS.reconfigure ASconfig @02:43:20.90 AS.seek 1ml @02:44:18.73 delay 1 second @02:44:19.74 AFV.select :holdingCoil @02:44:19.85 delay 1 second @02:44:20.88 AS.reconfigure ASslow0 @02:44:20.93 AS.seek :empty @02:46:01.89 AFV.select :carrierSolution @02:46:02.01 delay 2 seconds @02:46:04.04 AS.reconfigure ASconfig @02:46:04.09 AS.seek 1ml @02:47:01.89 delay 1 second @02:47:02.90 AFV.select :holdingCoil @02:47:03.01 delay 1 second @02:47:04.04 AS.reconfigure ASslow0 @02:47:04.08 AS.seek :empty @02:48:45.03 ATV.dial :air2 @02:48:45.68 AS.reconfigure ASslow1 @02:48:45.73 AS.seek 0.009ml @02:48:48.04 AS.reconfigure ASslow0 @02:48:48.09 AS.seek 1ml @02:50:28.03 ATV.dial AIV @02:50:28.77 AS.seek 0.02ml @02:52:07.64 ATV.dial :waste @02:52:09.17 AS.seek 0ml @02:52:11.49 AWV.select :air @02:52:11.66 Cleaning PCR @02:52:11.67 AFV.select :carrierSolution @02:52:11.78 delay 2 seconds @02:52:13.81 AS.reconfigure ASconfig @02:52:13.86 AS.seek 0.5ml @02:52:42.90 delay 1 second @02:52:43.91 AFV.select :holdingCoil @02:52:44.02 ASV.dial :waste1 @02:52:44.47 ATV.dial :air3 @02:52:45.12 AS.reconfigure ASslow1 @02:52:45.17 AS.seek 0.51ml @02:52:47.47 delay 2 seconds @02:52:49.48 ATV.dial :bleach @02:52:49.94 AS.seek 0.81ml @02:53:51.15 delay 3 seconds @02:53:54.17 ATV.dial :air3 @02:53:54.70 delay 3 seconds @02:53:57.79 AS.seek 0.835ml @02:54:03.09 delay 3 seconds @02:54:06.11 ATV.dial :bleach @02:54:06.71 AS.seek 0.82ml @02:54:10.03 delay 3 seconds @02:54:13.05 ARV.dial :waste @02:54:13.62 delay 3 seconds @02:54:16.64 ATV.dial :ARV @02:54:17.49 AS.reconfigure ASconfig @02:54:17.54 AS.seek 0.47ml @02:54:37.96 AFV.select :carrierSolution @02:54:38.08 delay 2 seconds @02:54:40.11 AS.seek 1ml @02:55:10.89 delay 1 second @02:55:11.90 AFV.select :holdingCoil @02:55:12.01 delay 1 second @02:55:13.04 AS.reconfigure ASfast @02:55:13.09 AS.seek 0ml @02:55:40.43 AFV.select :carrierSolution @02:55:40.55 delay 2 seconds @02:55:42.58 AS.reconfigure ASconfig @02:55:42.63 AS.seek 1ml @02:56:40.41 delay 1 second @02:56:41.42 AFV.select :holdingCoil @02:56:41.53 delay 1 second @02:56:42.56 AS.reconfigure ASfast @02:56:42.61 AS.seek 0ml @02:57:09.94 AFV.select :carrierSolution @02:57:10.06 delay 2 seconds @02:57:12.09 AS.reconfigure ASconfig @02:57:12.14 AS.seek 1ml @02:58:09.94 delay 1 second @02:58:10.96 AFV.select :holdingCoil @02:58:11.07 delay 1 second @02:58:12.10 AS.reconfigure ASfast @02:58:12.15 AS.seek 0ml @02:58:39.48 ARV.dial between carrier and air1 @02:58:39.97 ATV.dial :air3 @02:58:41.18 AS.reconfigure ASslow1 @02:58:41.23 AS.seek 0.01ml @02:58:43.64 delay 2 seconds @02:58:45.66 ATV.dial :bleach @02:58:46.27 AS.seek 0.81ml @03:01:27.50 delay 3 seconds @03:01:30.52 ATV.dial :air3 @03:01:31.05 delay 3 seconds @03:01:34.14 AS.seek 0.835ml @03:01:39.45 delay 3 seconds @03:01:42.48 ATV.dial :bleach @03:01:43.09 AS.seek 0.82ml @03:01:46.42 delay 3 seconds @03:01:49.44 ATV.dial PCR @03:01:49.83 AS.reconfigure ASconfig @03:01:49.88 AS.seek :empty @03:02:37.35 PCR.program :bleachProfile @03:02:37.42 PCR program should complete in < 2:45 @03:02:37.43 PCR.run 10:00 @03:05:57.43 PCR.off @03:05:57.46 AFV.select :carrierSolution @03:05:57.57 delay 2 seconds @03:05:59.60 AS.seek 1ml @03:06:57.44 delay 1 second @03:06:58.45 AFV.select :holdingCoil @03:06:58.56 delay 1 second @03:06:59.59 AS.reconfigure ASfast @03:06:59.64 AS.seek 0ml @03:07:26.99 delay 3 seconds @03:07:30.01 AFV.select :carrierSolution @03:07:30.12 delay 2 seconds @03:07:32.15 AS.reconfigure ASconfig @03:07:32.21 AS.seek 1ml @03:08:30.00 delay 1 second @03:08:31.02 AFV.select :holdingCoil @03:08:31.13 delay 1 second @03:08:32.16 AS.reconfigure ASfast @03:08:32.21 AS.seek 0ml @03:08:59.53 delay 3 seconds @03:09:02.55 AFV.select :carrierSolution @03:09:02.66 delay 2 seconds @03:09:04.69 AS.reconfigure ASconfig @03:09:04.76 AS.seek 1ml @03:10:02.55 delay 1 second @03:10:03.56 AFV.select :holdingCoil @03:10:03.67 delay 1 second @03:10:04.70 AS.reconfigure ASfast @03:10:04.75 AS.seek 0ml @03:10:32.10 delay 3 seconds @03:10:35.12 AFV.select :carrierSolution @03:10:35.23 delay 2 seconds @03:10:37.78 AS.reconfigure ASconfig @03:10:37.92 AS.seek 1ml @03:11:35.72 delay 1 second @03:11:36.73 AFV.select :holdingCoil @03:11:36.84 delay 1 second @03:11:37.87 AS.reconfigure ASfast @03:11:37.92 AS.seek 0ml @03:12:05.28 delay 3 seconds @03:12:08.30 AFV.select :carrierSolution @03:12:08.41 delay 2 seconds @03:12:10.44 AS.reconfigure ASconfig @03:12:10.49 AS.seek 1ml @03:13:08.29 delay 1 second @03:13:09.30 AFV.select :holdingCoil @03:13:09.41 delay 1 second @03:13:10.44 AS.reconfigure ASnorm @03:13:10.49 AS.seek 0ml @03:14:01.25 AFV.select :carrierSolution @03:14:01.36 delay 2 seconds @03:14:03.39 AS.reconfigure ASconfig @03:14:03.44 AS.seek 1ml @03:15:01.27 delay 1 second @03:15:02.29 AFV.select :holdingCoil @03:15:02.40 delay 1 second @03:15:03.42 ATV.dial :waste @03:15:04.57 AS.reconfigure ASnorm @03:15:04.63 AS.seek 0.5ml @03:15:30.20 ATV.dial :waste2 @03:15:31.41 AS.seek 0ml @03:15:56.96 AS.coast @03:15:57.00 SS.coast @03:15:57.04 CC.coast @03:15:57.08 CS.coast @03:15:57.13 PC.coast @03:15:57.17 PS.coast @03:15:57.21 Forearm.coast @03:15:57.25 Elbow.coast @03:15:57.65 Gate.power :core,:OFF @03:15:57.67 Gate.power :raw,:OFF @03:15:57.68 Shutdown @03:15:57.69 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @07:42:08.76 Session Begun @07:42:21.98 -> ESP.wakeup @07:42:22.04 Powering core bus @07:42:27.08 Gate.power :raw,:ON @07:42:27.79 SPE.coast @07:42:27.88 SPE.configure SPEconfig @07:42:28.00 AS.configure ASconfig @07:42:28.13 SS.configure SSconfig @07:42:28.26 CC.configure CCconfig @07:42:28.39 CS.configure CSconfig @07:42:28.52 PC.configure PCconfig @07:42:28.65 PS.configure PSconfig @07:42:28.79 Forearm.configure LooseForearm @07:42:28.92 Elbow.configure LooseElbow @07:42:29.07 SC.configure SCloose @07:42:29.20 SE.configure SEconfig @07:42:29.27 CH.coast @07:42:29.35 CH.configure CHconfig @07:42:29.41 PH.coast @07:42:29.50 PH.configure PHconfig @07:42:29.56 PCR.off @07:42:29.61 PCR.pidtemp= 50,0.1,0,300,25,25 @07:42:29.66 PCR.caltemp= 2.7073,0.0373,0 @07:42:29.72 All dwarf controllers running firmware version 3.75 @07:42:29.86 Valve.reset @07:42:30.06 PCR.off @07:42:30.11 PCR.pidtemp= 50,0.1,0,300,25,25 @07:42:30.17 PCR.caltemp= 2.7073,0.0373,0 @07:42:30.36 Intake.select :close @07:42:30.50 Exhaust.select :close @07:42:30.51 CTV.dial :air @07:42:30.62 PSV.dial :air @07:42:30.77 CSV.dial :waste1 @07:42:30.79 Forearm.reconfigure LooseForearm @07:42:30.93 Forearm.home.seek :retract @07:42:31.02 SSV.dial :spit,avoiding: :exhaust @07:42:31.28 PS.reconfigure PSconfig @07:42:31.35 PS.home.seek 0ml @07:42:32.03 Hand.close @07:42:32.08 CS.reconfigure CSfast @07:42:32.12 SS.reconfigure SSconfig @07:42:32.18 Elbow.jog 1500 @07:42:32.34 CS.home.seek 0ml @07:42:32.40 SS.home.seek 0ml @07:42:32.84 Elbow.reconfigure LooseElbow @07:42:32.91 Elbow.home.seek :clear @07:42:36.43 SE.reconfigure SEconfig @07:42:36.51 SE.home.seek :down @07:42:37.26 SC.reconfigure SCsloppy @07:42:37.33 SC.home.seek :initial @07:42:41.02 PS.coast @07:42:41.31 Forearm.seek :retract @07:42:41.42 Elbow.seek :hammer @07:42:42.73 Forearm.reconfigure TightForearm @07:42:42.81 Forearm.seek 5 + 350 counts @07:42:43.96 Forearm.seek :retract @07:42:45.24 PC.reconfigure PCconfig @07:42:45.27 CC.reconfigure CCconfig @07:42:45.37 PC.home.seek :open @07:42:45.47 CC.home.seek :open @07:43:12.38 -> MFB.ready? @07:43:24.97 -> MFB.ready! @07:43:25.08 Readying the MFB @07:43:25.10 Homing MFB Syringe @07:43:25.11 AFV.select :holdingCoil @07:43:25.30 ATV.dial :air2 @07:43:25.84 AS.reconfigure ASconfig @07:43:25.92 AS.home.seek :home @07:43:31.94 AFV.select :carrierSolution @07:43:32.08 delay 2 seconds @07:43:34.11 AS.seek 1ml @07:44:27.08 delay 1 second @07:44:28.09 AFV.select :holdingCoil @07:44:28.22 delay 1 second @07:44:29.25 ATV.dial :waste @07:44:30.23 AS.reconfigure ASnorm @07:44:30.30 AS.home.seek :empty @07:45:22.86 ATV.dial :waste @07:45:22.88 ARV.dial between carrier and air1 @07:45:23.02 ASV.dial :waste1 @07:46:49.76 -> ATV @07:46:55.95 -> ATV.ARV @07:46:55.97 ATV.dial ARV @07:47:01.71 -> ARV.air3 @07:47:01.74 ARV.dial :air3 @07:47:25.73 -> AS.pull 0.2, ASslow1 @07:47:25.83 AS.reconfigure ASslow1 @07:47:25.90 AS.seek 0.199ml @07:48:11.98 -> ATV @07:48:21.60 -> ARV.PP5 @07:48:21.63 ARV.dial :PP5 @07:49:34.54 -> AS.pull 0.075, ASslow1 @07:49:34.65 AS.seek 0.274ml @07:49:59.22 -> ARV.air3 @07:49:59.24 ARV.dial :air3 @07:50:21.21 -> AS.pull 0.2, ASslow1 @07:50:21.32 AS.seek 0.474ml @07:51:16.39 -> ATV.waste @07:51:16.40 ATV.dial :waste @07:51:33.03 -> AS.empty @07:51:33.05 AS.reconfigure ASconfig @07:51:33.13 AS.seek :empty @07:52:34.76 -> pcrDecon @07:52:34.86 Cleaning PCR @07:52:34.87 AFV.select :carrierSolution @07:52:35.01 delay 2 seconds @07:52:37.04 AS.seek 0.5ml @07:53:06.13 delay 1 second @07:53:07.14 AFV.select :holdingCoil @07:53:07.27 ASV.dial :waste1 @07:53:07.28 ATV.dial :air3 @07:53:08.20 AS.reconfigure ASslow1 @07:53:08.27 AS.seek 0.51ml @07:53:10.58 delay 2 seconds @07:53:12.59 ATV.dial :bleach @07:53:13.07 AS.seek 0.81ml @07:54:13.78 delay 3 seconds @07:54:16.80 ATV.dial :air3 @07:54:17.34 delay 3 seconds @07:54:20.43 AS.seek 0.835ml @07:54:26.25 delay 3 seconds @07:54:29.35 ATV.dial :bleach @07:54:29.96 AS.seek 0.82ml @07:54:33.27 delay 3 seconds @07:54:36.29 ARV.dial :waste @07:54:37.31 delay 3 seconds @07:54:40.33 ATV.dial :ARV @07:54:41.29 AS.reconfigure ASconfig @07:54:41.36 AS.seek 0.47ml @07:55:01.79 AFV.select :carrierSolution @07:55:01.93 delay 2 seconds @07:55:03.96 AS.seek 1ml @07:55:34.72 delay 1 second @07:55:35.74 AFV.select :holdingCoil @07:55:35.87 delay 1 second @07:55:36.90 AS.reconfigure ASfast @07:55:36.98 AS.seek 0ml @07:56:04.35 AFV.select :carrierSolution @07:56:04.49 delay 2 seconds @07:56:06.52 AS.reconfigure ASconfig @07:56:06.59 AS.seek 1ml @07:57:04.39 delay 1 second @07:57:05.41 AFV.select :holdingCoil @07:57:05.54 delay 1 second @07:57:06.57 AS.reconfigure ASfast @07:57:06.64 AS.seek 0ml @07:57:34.01 AFV.select :carrierSolution @07:57:34.15 delay 2 seconds @07:57:36.18 AS.reconfigure ASconfig @07:57:36.25 AS.seek 1ml @07:58:34.06 delay 1 second @07:58:35.07 AFV.select :holdingCoil @07:58:35.20 delay 1 second @07:58:36.23 AS.reconfigure ASfast @07:58:36.30 AS.seek 0ml @07:59:03.67 ARV.dial between carrier and air1 @07:59:04.17 ATV.dial :air3 @07:59:05.40 AS.reconfigure ASslow1 @07:59:05.47 AS.seek 0.01ml @07:59:07.83 delay 2 seconds @07:59:09.85 ATV.dial :bleach @07:59:10.48 AS.seek 0.81ml @08:01:51.67 delay 3 seconds @08:01:54.70 ATV.dial :air3 @08:01:55.24 delay 3 seconds @08:01:58.33 AS.seek 0.835ml @08:02:03.67 delay 3 seconds @08:02:06.69 ATV.dial :bleach @08:02:07.31 AS.seek 0.82ml @08:02:10.64 delay 3 seconds @08:02:13.66 ATV.dial PCR @08:02:14.07 AS.reconfigure ASconfig @08:02:14.14 AS.seek :empty @08:03:01.63 PCR.program :bleachProfile @08:03:01.71 PCR program should complete in < 2:45 @08:03:01.72 PCR.run 10:00 @08:06:34.22 PCR.off @08:06:34.26 AFV.select :carrierSolution @08:06:34.39 delay 2 seconds @08:06:36.42 AS.seek 1ml @08:07:34.24 delay 1 second @08:07:35.26 AFV.select :holdingCoil @08:07:35.30 Errno::EPIPE in mwj's_reader -- Broken pipe @08:07:35.34 Session Ended @08:11:11.08 Session Begun @08:11:25.71 -> showlog 50 @08:12:22.50 -> showlog 50 @08:12:41.28 -> AS @08:14:14.39 -> ATV @08:15:10.64 -> ASV @08:15:55.82 -> AS @08:16:07.00 -> AFV @08:19:00.83 -> AS.empty @08:19:00.86 AS.seek :empty @08:20:16.61 -> AFV.carrierSolution @08:20:16.62 AFV.select :carrierSolution @08:20:39.95 -> AS.full @08:20:39.98 NoMethodError in mwj -- undefined method `full' for AS:Syringe @08:22:00.21 -> AS.pull 1, ASfast @08:22:00.31 AS.reconfigure ASfast @08:22:00.39 AS.seek 0.999ml @08:28:32.91 Session Begun @08:28:34.86 Session Ended @08:28:49.29 -> AS @08:29:04.30 -> AFV @08:30:42.25 -> AFV.holdingCoil @08:30:42.27 AFV.select :holdingCoil @08:31:12.26 -> AS.empty @08:31:12.29 AS.reconfigure ASconfig @08:31:12.37 AS.seek :empty @08:34:56.74 Session Ended @08:39:25.29 Session Begun @08:39:38.31 -> AFV.carrierSolution @08:39:38.32 AFV.select :carrierSolution @08:40:03.83 -> AS.pull 1, ASfast @08:40:03.93 AS.reconfigure ASfast @08:40:04.01 AS.seek 0.999ml @08:40:45.89 -> AFV.holdingCoil @08:40:45.90 AFV.select :holdingCoil @08:41:00.94 -> ATV @08:41:06.40 -> ASV @08:41:18.42 -> AS.empty @08:41:18.44 AS.reconfigure ASconfig @08:41:18.55 AS.seek :empty @08:43:06.17 -> AFV.carrierSolution @08:43:06.19 AFV.select :carrierSolution @08:43:37.63 -> AS.pull 1, ASfast @08:43:37.74 AS.reconfigure ASfast @08:43:37.81 AS.seek 0.999ml @08:45:08.93 -> AFV.holdingCoil @08:45:08.95 AFV.select :holdingCoil @08:45:22.94 -> ATV.waste @08:45:22.97 ATV.dial :waste @08:45:51.47 -> AS.push 0.5, ASfast @08:45:51.58 AS.seek 0.499ml @08:46:20.94 -> ATV @08:46:32.50 -> ATV.waste2 @08:46:32.52 ATV.dial :waste2 @08:46:41.89 -> AS.empty @08:46:41.91 AS.reconfigure ASconfig @08:46:41.99 AS.seek :empty @08:47:21.35 -> ATV @08:47:49.75 -> ESP.shutdown @08:47:49.78 AS.coast @08:47:49.82 Asleep / Shutdown @08:47:49.90 SS.coast @08:47:49.96 CC.coast @08:47:50.02 CS.coast @08:47:50.08 PC.coast @08:47:50.14 Forearm.coast @08:47:50.20 Elbow.coast @08:47:50.26 SC.coast @08:47:50.32 SE.coast @08:47:50.73 Gate.power :core,:OFF @08:47:50.75 Gate.power :raw,:OFF @08:47:50.77 Shutdown @08:47:57.44 -> exit @08:47:57.46 Session Ended @08:59:11.52 Session Begun @09:01:27.50 Session Ended @09:04:17.72 Session Begun @09:07:31.05 -> $dilmfb[:dilution] = 0.2 @09:08:11.29 -> exit @09:08:11.30 Session Ended @14:00:00.05
Powering core bus @14:00:04.93 Gate.power :raw,:ON @14:00:05.60 SPE.coast @14:00:05.67 SPE.configure SPEconfig @14:00:05.76 AS.configure ASconfig @14:00:05.87 SS.configure SSconfig @14:00:05.97 CC.configure CCconfig @14:00:06.07 CS.configure CSconfig @14:00:06.17 PC.configure PCconfig @14:00:06.27 PS.configure PSconfig @14:00:06.40 Forearm.configure LooseForearm @14:00:06.50 Elbow.configure LooseElbow @14:00:06.62 SC.configure SCloose @14:00:06.72 SE.configure SEconfig @14:00:06.76 CH.coast @14:00:06.82 CH.configure CHconfig @14:00:06.85 PH.coast @14:00:06.91 PH.configure PHconfig @14:00:06.96 PCR.off @14:00:06.99 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:07.01 PCR.caltemp= 2.7073,0.0373,0 @14:00:07.04 All dwarf controllers running firmware version 3.75 @14:00:07.16 Valve.reset @14:00:07.33 PCR.off @14:00:07.36 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:07.39 PCR.caltemp= 2.7073,0.0373,0 @14:00:07.60 Intake.select :close @14:00:07.62 Exhaust.select :close @14:00:07.63 CTV.dial :air @14:00:07.72 PSV.dial :air @14:00:07.76 CSV.dial :waste1 @14:00:07.92
Forearm.reconfigure LooseForearm @14:00:07.98 Forearm.home.seek :retract @14:00:08.01 SSV.dial :spit,avoiding: :exhaust @14:00:08.14 PS.reconfigure PSconfig @14:00:08.24 PS.home.seek 0ml @14:00:08.30 CS.reconfigure CSfast @14:00:08.34 SS.reconfigure SSconfig @14:00:08.44 SS.home.seek 0ml @14:00:08.45 CS.home.seek 0ml @14:00:08.79 Hand.close @14:00:08.85
Elbow.jog 1500 @14:00:09.32 Elbow.reconfigure LooseElbow @14:00:09.36 Elbow.home.seek :clear @14:00:14.05 SE.reconfigure SEconfig @14:00:14.10 SE.home.seek :down @14:00:14.70 SC.reconfigure SCsloppy @14:00:14.75 SC.home.seek :initial @14:00:17.27 PS.coast @14:00:19.16
Forearm.seek :retract @14:00:19.33 Elbow.seek :hammer @14:00:20.60 Forearm.reconfigure TightForearm @14:00:20.65 Forearm.seek 5 + 350 counts @14:00:21.81 Forearm.seek :retract @14:00:23.02 CC.reconfigure CCconfig @14:00:23.04 PC.reconfigure PCconfig @14:00:23.13
CC.home.seek :open @14:00:23.20 PC.home.seek :open @14:00:26.59
Phase [phasewithdil] @14:00:26.61 GoodNews.email "At 14:00:26 began in tube 3: phasewithdil\n/home/esp/esp2/mission/18tundeployv2dil.rb:118\n{1 => 22, 2 => 2, 3 => 20}\nCan@14:00:26, 13.7C, 69% humidity, 14.3psia, 13.500V, 0.796A, 0.796A avg, 10.75W",Subject: "Started 18tundeployv2dil phase phasewithdil" @14:00:26.63CEST08-Oct-18
==================> STARTED Superphasewdil (phase #5) <=== @14:00:26.65 Starting initialPurge @14:00:26.67 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:00:26.75 Hand.open @14:00:27.46 Elbow.reconfigure TightElbow @14:00:27.51 Elbow.seek FlushPuck::Garage @14:00:29.44 Elbow.reconfigure LooseElbow @14:00:29.54 Forearm.reconfigure LooseForearm @14:00:29.59 Forearm.seek :garage @14:00:30.55 Hand.close @14:00:31.34 Forearm.seek :retract @14:00:32.32 Elbow.reconfigure TightElbow @14:00:32.37 Elbow.seek CC @14:00:35.27 Elbow.reconfigure LooseElbow @14:00:35.40 Forearm.reconfigure TightForearm @14:00:35.48 Forearm.seek CC @14:00:36.79 CC.seek :closed @14:00:41.04 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.04 @14:00:41.13 Hand.open @14:00:41.77 Forearm.reconfigure LooseForearm @14:00:41.82 Forearm.seek :retract @14:00:42.87 Flushing the Collection Valve::Manifold @14:00:43.02 CSV.dial CSR @14:00:44.45 Pulling 5ml of CSR.flush @14:00:44.46 CSR.select :flush @14:00:44.59 CS.reconfigure CSconfig @14:00:44.63 CS.seek 5ml @14:00:58.56 delay 5 seconds @14:01:03.58 CSR.select :air @14:01:03.77 CS.reconfigure CSfast @14:01:03.82 CS.seek 7.5ml @14:01:10.64 CSV.dial :waste @14:01:12.01 CS.reconfigure CSconfig @14:01:12.09 CS.seek 0ml @14:01:32.28 Purging external Intake @14:01:32.41 CSV.dial :flush @14:01:32.89 CS.reconfigure CSfast @14:01:32.94 CS.seek 10ml @14:01:56.84 delay 5 seconds @14:02:01.87 CSV.dial CBV @14:02:01.93
CBV.dial CSV @14:02:02.73 CTV.dial :intake @14:02:03.49 Intake.select :open @14:02:03.62 CS.seek 0ml @14:02:28.33 Intake.select :close @14:02:28.45 CTV.dial :air @14:02:29.25 Flushing upper manifold @14:02:29.38 CSV.dial :flush @14:02:30.67 CS.seek 5ml @14:02:43.00 delay 5 seconds @14:02:48.02 CSV.dial :air @14:02:48.57 CS.seek 7ml @14:02:54.22 CSV.dial CBV @14:02:54.25
CBV.dial CSV @14:02:55.32 CTV.dial :air @14:02:55.34 CS.seek 0ml @14:03:12.31 delay 7 seconds @14:03:19.35 CS.seek 3ml @14:03:27.17 CSV.dial :waste @14:03:27.66 CS.seek 0ml @14:03:35.65 CSV.dial :flush @14:03:36.13 CS.seek 3ml @14:03:43.97 delay 5 seconds @14:03:48.99 CSV.dial :air @14:03:49.54 CS.seek 5ml @14:03:55.11 CSV.dial :air @14:03:55.14 CS.seek 0ml @14:04:07.56 delay 7 seconds @14:04:14.60 CS.seek 3ml @14:04:22.43 CSV.dial :waste @14:04:23.21 CS.seek 0ml @14:04:31.03 Garaging FlushPuck in Collection Clamp @14:04:31.11 Elbow.reconfigure TightElbow @14:04:31.16 Elbow.seek CC @14:04:31.40 Elbow.reconfigure LooseElbow @14:04:31.51 Forearm.seek CC @14:04:32.50 Hand.close @14:04:33.08 CC.seek :open @14:04:37.47 Forearm.seek :retract @14:04:38.50 Elbow.reconfigure TightElbow @14:04:38.55 Elbow.seek :garage @14:04:41.64 Elbow.reconfigure LooseElbow @14:04:41.74 Forearm.reconfigure TightForearm @14:04:41.79 Forearm.seek :garage @14:04:42.94 Hand.open @14:04:43.65 Forearm.reconfigure LooseForearm @14:04:43.69 Forearm.seek :retract @14:04:44.79 Starting mfb @14:04:44.81 pcrBAC sampling at most 1500ml, wcr at most 1500ml @14:04:44.83 BACfans.email "Started mfb in tube 3 at 14:04:44CEST08-Oct-18\n{1 => 22, 2 => 2, 3 => 20}\nCan@14:00:26, 13.7C, 69% humidity, 14.3psia, 13.500V, 0.796A, 0.796A avg, 10.75W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @14:04:44.91
Loading sh1 puck from tube 3 into Collection Clamp @14:04:45.05 Readying the MFB @14:04:45.09 Homing MFB Syringe @14:04:45.13 AFV.select :holdingCoil @14:04:45.37 ATV.dial :air2 @14:04:45.40
SC.reconfigure SCtight @14:04:45.50 SC.seek 3 @14:04:45.95 AS.reconfigure ASconfig @14:04:46.00 AS.home.seek :home @14:04:47.56
SC.reconfigure SCloose @14:04:47.79 Elbow.reconfigure TightElbow @14:04:47.84 Elbow.seek 3 @14:04:49.86 Forearm.reconfigure TightForearm @14:04:49.92 Forearm.seek 3 + 700 counts @14:04:51.04 SE.reconfigure SEpick @14:04:51.11 SE.seek beamBreak @14:04:51.94 AFV.select :carrierSolution @14:04:52.12 delay 2 seconds @14:04:52.41
SE.configure SEconfig @14:04:52.60 Elbow.seek 3 @14:04:52.81 Forearm.seek 3 + 300 counts @14:04:53.11 Forearm.reconfigure LooseForearm @14:04:53.16 Hand.close @14:04:53.85 SE.seek :down @14:04:54.12
Forearm.seek :retract @14:04:54.20 AS.seek 1ml @14:04:54.88 SE.coast @14:04:55.08
Elbow.seek CC @14:04:56.71 Elbow.reconfigure LooseElbow @14:04:56.83 Forearm.reconfigure TightForearm @14:04:56.88 Forearm.seek CC @14:04:58.19 CC.seek :closed @14:05:02.43 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.10 @14:05:02.44 Hand.open @14:05:03.15 Forearm.reconfigure LooseForearm @14:05:03.21 Forearm.seek :retract @14:05:04.20 SC.coast @14:05:04.24 collectSample 1500ml w/bubble point of 55psi @14:05:04.25 Priming 7ml @14:05:04.26 Intake.select :close @14:05:04.27 Exhaust.select :close @14:05:04.29 SS.hold @14:05:04.53 Calibrating pressure and temperature sensors @14:05:04.55 CTV.dial :air @14:05:04.56 SSV.dial :spit,avoiding: :exhaust @14:05:04.58 SS.reconfigure SSfast @14:05:04.63 SS.seek 5ml @14:05:07.53 SSV.dial CBV @14:05:07.56
CBV.dial SSV @14:05:08.95 delay 3.8 seconds @14:05:12.95 SSV.dial :spit @14:05:14.32 SS.seek 0ml @14:05:17.05 SSV.dial CBV @14:05:18.43 CTV.dial :intake @14:05:19.22 SSV.dial CBV @14:05:19.25
CBV.dial SSV @14:05:19.26 Allowing 40s for 7.0ml of remaining 7.0ml @14:05:19.27 Intake.select :open @14:05:21.46 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:05:21.47 SS.reconfigure SSinhale.with maxSpeed: 150 @14:05:21.52 SS.seek 7ml @14:05:25.27 Vacuum too high -- waiting up to 40s @14:05:26.89 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:05:26.91 SS.reconfigure SSinhale.with maxSpeed: 135 @14:05:26.96 SS.seek 7ml @14:05:29.57 Vacuum too high -- waiting up to 40s @14:05:30.70 Intake @31.9psia, vacuum from 20.0 to 24.0psi @14:05:30.72 SS.reconfigure SSinhale.with maxSpeed: 122 @14:05:30.77 SS.seek 7ml @14:05:32.31 Stroke completed -- waiting for pressure to equalize ... @14:05:34.36 Intake.select :close @14:05:34.52 SSV.dial :exhaust @14:05:35.33 Exhaust.select :open @14:05:35.44 SS.reconfigure SSexhale @14:05:35.49 SS.seek 0ml @14:05:39.28 Exhaust.select :close @14:05:39.40 Sampling 1500.0ml @14:05:39.41 Intake.select :close @14:05:39.42 Exhaust.select :close @14:05:39.44 SS.reconfigure SSconfig @14:05:39.48 SS.hold @14:05:39.71 CTV.dial :intake @14:05:39.72 SSV.dial CBV @14:05:39.75
CBV.dial SSV @14:05:40.58 Allowing 130s for 25.0ml of remaining 1500.0ml @14:05:40.59 Intake.select :open @14:05:42.78 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:05:42.79 SS.reconfigure SSinhale.with maxSpeed: 122 @14:05:42.84 SS.seek 25ml @14:05:46.38 Vacuum too high -- waiting up to 40s @14:05:47.18 delay 1 second @14:05:47.75
Intake @32.9psia, vacuum from 20.0 to 24.0psi @14:05:47.77 SS.reconfigure SSinhale.with maxSpeed: 110 @14:05:47.82 SS.seek 25ml @14:05:48.20 AFV.select :holdingCoil @14:05:48.31 delay 1 second @14:05:49.34 ATV.dial :waste @14:05:50.31 AS.reconfigure ASnorm @14:05:50.36 AS.home.seek :empty @14:05:50.54
Vacuum too high -- waiting up to 40s @14:05:51.90 Intake @32.4psia, vacuum from 20.0 to 24.0psi @14:05:51.92 SS.reconfigure SSinhale.with maxSpeed: 99 @14:05:51.97 SS.seek 25ml @14:05:54.85 Vacuum too high -- waiting up to 40s @14:05:56.45 Intake @32.8psia, vacuum from 20.0 to 24.0psi @14:05:56.47 SS.reconfigure SSinhale.with maxSpeed: 89 @14:05:56.53 SS.seek 25ml @14:05:59.76 Vacuum too high -- waiting up to 40s @14:06:01.66 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:06:01.68 SS.reconfigure SSinhale.with maxSpeed: 80 @14:06:01.73 SS.seek 25ml @14:06:05.52 Vacuum too high -- waiting up to 40s @14:06:06.63 Intake @32.6psia, vacuum from 20.0 to 24.0psi @14:06:06.65 SS.reconfigure SSinhale.with maxSpeed: 72 @14:06:06.70 SS.seek 25ml @14:06:10.74 Vacuum too high -- waiting up to 40s @14:06:11.85 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:06:11.87 SS.reconfigure SSinhale.with maxSpeed: 65 @14:06:11.92 SS.seek 25ml @14:06:16.14 Vacuum too high -- waiting up to 40s @14:06:17.23 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:06:17.25 SS.reconfigure SSinhale.with maxSpeed: 59 @14:06:17.30 SS.seek 25ml @14:06:20.86 Stroke completed -- waiting for pressure to equalize ... @14:06:23.47 Intake.select :close @14:06:23.63 SSV.dial :exhaust @14:06:24.43 Exhaust.select :open @14:06:24.54 SS.reconfigure SSexhale @14:06:24.59 SS.seek 0ml @14:06:33.41 Exhaust.select :close @14:06:33.53 SSV.dial CBV @14:06:33.56
CBV.dial SSV @14:06:34.39 Allowing 130s for 25.0ml of remaining 1475.0ml @14:06:34.40 Intake.select :open @14:06:36.57 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:06:36.59 SS.reconfigure SSinhale.with maxSpeed: 59 @14:06:36.64 SS.seek 25ml @14:06:42.91 ATV.dial :waste @14:06:42.93 ARV.dial between carrier and air1 @14:06:43.01 ASV.dial :waste1 @14:06:43.82
Vacuum too high -- waiting up to 40s @14:06:44.65 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:06:44.67 SS.reconfigure SSinhale.with maxSpeed: 53 @14:06:44.72 SS.seek 25ml @14:07:11.56 Stroke completed -- waiting for pressure to equalize ... @14:07:14.15 Intake.select :close @14:07:14.31 SSV.dial :exhaust @14:07:15.10 Exhaust.select :open @14:07:15.22 SS.reconfigure SSexhale @14:07:15.27 SS.seek 0ml @14:07:24.43 Exhaust.select :close @14:07:24.54 SSV.dial CBV @14:07:24.57
CBV.dial SSV @14:07:25.37 Allowing 130s for 25.0ml of remaining 1450.0ml @14:07:25.38 Intake.select :open @14:07:27.56 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:07:27.57 SS.reconfigure SSinhale.with maxSpeed: 53 @14:07:27.62 SS.seek 25ml @14:07:43.79 Vacuum too high -- waiting up to 40s @14:07:44.90 Intake @32.0psia, vacuum from 20.0 to 24.0psi @14:07:44.94 SS.reconfigure SSinhale.with maxSpeed: 48 @14:07:44.99 SS.seek 25ml @14:08:05.53 Stroke completed -- waiting for pressure to equalize ... @14:08:07.50 Intake.select :close @14:08:07.66 SSV.dial :exhaust @14:08:08.46 Exhaust.select :open @14:08:08.57 SS.reconfigure SSexhale @14:08:08.62 SS.seek 0ml @14:08:17.35 Exhaust.select :close @14:08:17.47 SSV.dial CBV @14:08:17.50
CBV.dial SSV @14:08:18.32 Allowing 130s for 25.0ml of remaining 1425.0ml @14:08:18.33 Intake.select :open @14:08:20.50 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:08:20.52 SS.reconfigure SSinhale.with maxSpeed: 48 @14:08:20.57 SS.seek 25ml @14:08:58.08 Stroke completed -- waiting for pressure to equalize ... @14:09:00.68 Intake.select :close @14:09:00.84 SSV.dial :exhaust @14:09:01.64 Exhaust.select :open @14:09:01.75 SS.reconfigure SSexhale @14:09:01.80 SS.seek 0ml @14:09:10.78 Exhaust.select :close @14:09:10.90 SSV.dial CBV @14:09:10.93
CBV.dial SSV @14:09:11.75 Allowing 130s for 25.0ml of remaining 1400.0ml @14:09:11.76 Intake.select :open @14:09:13.93 Intake @32.6psia, vacuum from 20.0 to 24.0psi @14:09:13.95 SS.reconfigure SSinhale.with maxSpeed: 48 @14:09:14.00 SS.seek 25ml @14:09:51.27 Stroke completed -- waiting for pressure to equalize ... @14:09:53.85 Intake.select :close @14:09:54.01 SSV.dial :exhaust @14:09:54.79 Exhaust.select :open @14:09:54.90 SS.reconfigure SSexhale @14:09:54.95 SS.seek 0ml @14:10:03.72 Exhaust.select :close @14:10:03.84 SSV.dial CBV @14:10:03.87
CBV.dial SSV @14:10:04.68 Allowing 130s for 25.0ml of remaining 1375.0ml @14:10:04.69 Intake.select :open @14:10:06.86 Intake @32.3psia, vacuum from 20.0 to 24.0psi @14:10:06.88 SS.reconfigure SSinhale.with maxSpeed: 48 @14:10:06.93 SS.seek 25ml @14:10:44.31 Stroke completed -- waiting for pressure to equalize ... @14:10:46.88 Intake.select :close @14:10:47.03 SSV.dial :exhaust @14:10:47.81 Exhaust.select :open @14:10:47.93 SS.reconfigure SSexhale @14:10:47.98 SS.seek 0ml @14:10:56.80 Exhaust.select :close @14:10:56.92 SSV.dial CBV @14:10:56.95
CBV.dial SSV @14:10:57.76 Allowing 130s for 25.0ml of remaining 1350.0ml @14:10:57.77 Intake.select :open @14:10:59.94 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:10:59.96 SS.reconfigure SSinhale.with maxSpeed: 48 @14:11:00.01 SS.seek 25ml @14:11:37.56 Stroke completed -- waiting for pressure to equalize ... @14:11:40.14 Intake.select :close @14:11:40.82 SSV.dial :exhaust @14:11:41.62 Exhaust.select :open @14:11:41.73 SS.reconfigure SSexhale @14:11:41.78 SS.seek 0ml @14:11:50.85 Exhaust.select :close @14:11:50.97 SSV.dial CBV @14:11:51.00
CBV.dial SSV @14:11:51.81 Allowing 130s for 25.0ml of remaining 1325.0ml @14:11:51.82 Intake.select :open @14:11:54.00 Intake @34.4psia, vacuum from 20.0 to 24.0psi @14:11:54.02 SS.reconfigure SSinhale.with maxSpeed: 48 @14:11:54.07 SS.seek 25ml @14:12:31.41 Stroke completed -- waiting for pressure to equalize ... @14:12:34.01 Intake.select :close @14:12:34.17 SSV.dial :exhaust @14:12:34.98 Exhaust.select :open @14:12:35.09 SS.reconfigure SSexhale @14:12:35.14 SS.seek 0ml @14:12:44.31 Exhaust.select :close @14:12:44.43 SSV.dial CBV @14:12:44.46
CBV.dial SSV @14:12:45.26 Allowing 130s for 25.0ml of remaining 1300.0ml @14:12:45.27 Intake.select :open @14:12:47.44 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:12:47.46 SS.reconfigure SSinhale.with maxSpeed: 48 @14:12:47.51 SS.seek 25ml @14:13:24.72 Stroke completed -- waiting for pressure to equalize ... @14:13:27.32 Intake.select :close @14:13:27.48 SSV.dial :exhaust @14:13:28.29 Exhaust.select :open @14:13:28.40 SS.reconfigure SSexhale @14:13:28.45 SS.seek 0ml @14:13:37.42 Exhaust.select :close @14:13:37.53 SSV.dial CBV @14:13:37.56
CBV.dial SSV @14:13:38.39 Allowing 130s for 25.0ml of remaining 1275.0ml @14:13:38.40 Intake.select :open @14:13:40.58 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:13:40.60 SS.reconfigure SSinhale.with maxSpeed: 48 @14:13:40.65 SS.seek 25ml @14:14:17.97 Stroke completed -- waiting for pressure to equalize ... @14:14:20.63 Intake.select :close @14:14:20.78 SSV.dial :exhaust @14:14:21.57 Exhaust.select :open @14:14:21.68 SS.reconfigure SSexhale @14:14:21.74 SS.seek 0ml @14:14:30.74 Exhaust.select :close @14:14:30.86 SSV.dial CBV @14:14:30.89
CBV.dial SSV @14:14:31.71 Allowing 130s for 25.0ml of remaining 1250.0ml @14:14:31.72 Intake.select :open @14:14:33.89 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:14:33.91 SS.reconfigure SSinhale.with maxSpeed: 48 @14:14:33.96 SS.seek 25ml @14:15:11.20 Stroke completed -- waiting for pressure to equalize ... @14:15:13.78 Intake.select :close @14:15:13.94 SSV.dial :exhaust @14:15:14.72 Exhaust.select :open @14:15:14.84 SS.reconfigure SSexhale @14:15:14.90 SS.seek 0ml @14:15:23.97 Exhaust.select :close @14:15:24.09 SSV.dial CBV @14:15:24.12
CBV.dial SSV @14:15:24.92 Allowing 130s for 25.0ml of remaining 1225.0ml @14:15:24.93 Intake.select :open @14:15:27.62 Intake @34.6psia, vacuum from 20.0 to 24.0psi @14:15:27.64 SS.reconfigure SSinhale.with maxSpeed: 48 @14:15:27.69 SS.seek 25ml @14:15:48.23 Vacuum too high -- waiting up to 40s @14:15:48.83 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:15:48.85 SS.reconfigure SSinhale.with maxSpeed: 43 @14:15:48.90 SS.seek 25ml @14:16:07.78 Stroke completed -- waiting for pressure to equalize ... @14:16:09.74 Intake.select :close @14:16:09.90 SSV.dial :exhaust @14:16:10.70 Exhaust.select :open @14:16:10.82 SS.reconfigure SSexhale @14:16:10.86 SS.seek 0ml @14:16:20.05 Exhaust.select :close @14:16:20.17 SSV.dial CBV @14:16:20.20
CBV.dial SSV @14:16:21.01 Allowing 130s for 25.0ml of remaining 1200.0ml @14:16:21.02 Intake.select :open @14:16:23.19 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:16:23.21 SS.reconfigure SSinhale.with maxSpeed: 43 @14:16:23.26 SS.seek 25ml @14:17:04.52 Stroke completed -- waiting for pressure to equalize ... @14:17:07.12 Intake.select :close @14:17:07.29 SSV.dial :exhaust @14:17:08.09 Exhaust.select :open @14:17:08.20 SS.reconfigure SSexhale @14:17:08.25 SS.seek 0ml @14:17:17.16 Exhaust.select :close @14:17:17.28 SSV.dial CBV @14:17:17.31
CBV.dial SSV @14:17:18.14 Allowing 130s for 25.0ml of remaining 1175.0ml @14:17:18.15 Intake.select :open @14:17:20.32 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:17:20.34 SS.reconfigure SSinhale.with maxSpeed: 43 @14:17:20.39 SS.seek 25ml @14:18:01.95 Stroke completed -- waiting for pressure to equalize ... @14:18:04.53 Intake.select :close @14:18:04.69 SSV.dial :exhaust @14:18:05.50 Exhaust.select :open @14:18:05.61 SS.reconfigure SSexhale @14:18:05.66 SS.seek 0ml @14:18:14.89 Exhaust.select :close @14:18:15.01 SSV.dial CBV @14:18:15.04
CBV.dial SSV @14:18:15.84 Allowing 130s for 25.0ml of remaining 1150.0ml @14:18:15.85 Intake.select :open @14:18:18.02 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:18:18.04 SS.reconfigure SSinhale.with maxSpeed: 43 @14:18:18.09 SS.seek 25ml @14:18:59.49 Stroke completed -- waiting for pressure to equalize ... @14:19:02.06 Intake.select :close @14:19:02.21 SSV.dial :exhaust @14:19:03.03 Exhaust.select :open @14:19:03.14 SS.reconfigure SSexhale @14:19:03.19 SS.seek 0ml @14:19:11.88 Exhaust.select :close @14:19:12.00 SSV.dial CBV @14:19:12.03
CBV.dial SSV @14:19:12.86 Allowing 130s for 25.0ml of remaining 1125.0ml @14:19:12.87 Intake.select :open @14:19:15.04 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:19:15.06 SS.reconfigure SSinhale.with maxSpeed: 43 @14:19:15.11 SS.seek 25ml @14:19:56.75 Stroke completed -- waiting for pressure to equalize ... @14:19:59.24 Intake.select :close @14:19:59.40 SSV.dial :exhaust @14:20:00.20 Exhaust.select :open @14:20:00.31 SS.reconfigure SSexhale @14:20:00.36 SS.seek 0ml @14:20:09.55 Exhaust.select :close @14:20:09.67 SSV.dial CBV @14:20:09.70
CBV.dial SSV @14:20:10.50 Allowing 130s for 25.0ml of remaining 1100.0ml @14:20:10.51 Intake.select :open @14:20:12.68 Intake @34.5psia, vacuum from 20.0 to 24.0psi @14:20:12.70 SS.reconfigure SSinhale.with maxSpeed: 43 @14:20:12.75 SS.seek 25ml @14:20:54.11 Stroke completed -- waiting for pressure to equalize ... @14:20:56.72 Intake.select :close @14:20:56.88 SSV.dial :exhaust @14:20:57.68 Exhaust.select :open @14:20:57.79 SS.reconfigure SSexhale @14:20:57.84 SS.seek 0ml @14:21:07.00 Exhaust.select :close @14:21:07.12 SSV.dial CBV @14:21:07.15
CBV.dial SSV @14:21:07.96 Allowing 130s for 25.0ml of remaining 1075.0ml @14:21:07.97 Intake.select :open @14:21:10.14 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:21:10.16 SS.reconfigure SSinhale.with maxSpeed: 43 @14:21:10.22 SS.seek 25ml @14:21:51.31 Stroke completed -- waiting for pressure to equalize ... @14:21:53.90 Intake.select :close @14:21:54.05 SSV.dial :exhaust @14:21:54.85 Exhaust.select :open @14:21:54.97 SS.reconfigure SSexhale @14:21:55.03 SS.seek 0ml @14:22:04.08 Exhaust.select :close @14:22:04.20 SSV.dial CBV @14:22:04.25
CBV.dial SSV @14:22:05.01 Allowing 130s for 25.0ml of remaining 1050.0ml @14:22:05.02 Intake.select :open @14:22:07.20 Intake @32.8psia, vacuum from 20.0 to 24.0psi @14:22:07.21 SS.reconfigure SSinhale.with maxSpeed: 43 @14:22:07.26 SS.seek 25ml @14:22:48.61 Stroke completed -- waiting for pressure to equalize ... @14:22:51.79 Intake.select :close @14:22:51.95 SSV.dial :exhaust @14:22:52.75 Exhaust.select :open @14:22:52.87 SS.reconfigure SSexhale @14:22:52.92 SS.seek 0ml @14:23:01.53 Exhaust.select :close @14:23:01.65 SSV.dial CBV @14:23:01.70
CBV.dial SSV @14:23:02.48 Allowing 130s for 25.0ml of remaining 1025.0ml @14:23:02.49 Intake.select :open @14:23:04.66 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:23:04.68 SS.reconfigure SSinhale.with maxSpeed: 43 @14:23:04.73 SS.seek 25ml @14:23:39.73 Vacuum too high -- waiting up to 40s @14:23:40.30 Intake @31.7psia, vacuum from 20.0 to 24.0psi @14:23:40.32 SS.reconfigure SSinhale.with maxSpeed: 39 @14:23:40.37 SS.seek 25ml @14:23:47.89 Stroke completed -- waiting for pressure to equalize ... @14:23:50.37 Intake.select :close @14:23:50.53 SSV.dial :exhaust @14:23:51.32 Exhaust.select :open @14:23:51.43 SS.reconfigure SSexhale @14:23:51.48 SS.seek 0ml @14:24:00.50 Exhaust.select :close @14:24:00.62 SSV.dial CBV @14:24:00.65
CBV.dial SSV @14:24:01.45 Allowing 130s for 25.0ml of remaining 1000.0ml @14:24:01.46 Intake.select :open @14:24:04.00 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:24:04.02 SS.reconfigure SSinhale.with maxSpeed: 39 @14:24:04.07 SS.seek 25ml @14:24:49.47 Stroke completed -- waiting for pressure to equalize ... @14:24:52.07 Intake.select :close @14:24:52.23 SSV.dial :exhaust @14:24:53.04 Exhaust.select :open @14:24:53.15 SS.reconfigure SSexhale @14:24:53.21 SS.seek 0ml @14:25:02.31 Exhaust.select :close @14:25:02.42 SSV.dial CBV @14:25:02.45
CBV.dial SSV @14:25:03.26 Allowing 130s for 25.0ml of remaining 975.0ml @14:25:03.27 Intake.select :open @14:25:05.44 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:25:05.46 SS.reconfigure SSinhale.with maxSpeed: 39 @14:25:05.51 SS.seek 25ml @14:25:50.89 Stroke completed -- waiting for pressure to equalize ... @14:25:54.13 Intake.select :close @14:25:54.29 SSV.dial :exhaust @14:25:55.09 Exhaust.select :open @14:25:55.20 SS.reconfigure SSexhale @14:25:55.26 SS.seek 0ml @14:26:04.14 Exhaust.select :close @14:26:04.26 SSV.dial CBV @14:26:04.29
CBV.dial SSV @14:26:05.10 Allowing 130s for 25.0ml of remaining 950.0ml @14:26:05.11 Intake.select :open @14:26:07.28 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:26:07.30 SS.reconfigure SSinhale.with maxSpeed: 39 @14:26:07.35 SS.seek 25ml @14:26:53.13 Stroke completed -- waiting for pressure to equalize ... @14:26:56.33 Intake.select :close @14:26:56.49 SSV.dial :exhaust @14:26:57.28 Exhaust.select :open @14:26:57.39 SS.reconfigure SSexhale @14:26:57.45 SS.seek 0ml @14:27:06.53 Exhaust.select :close @14:27:06.65 SSV.dial CBV @14:27:06.68
CBV.dial SSV @14:27:07.48 Allowing 130s for 25.0ml of remaining 925.0ml @14:27:07.49 Intake.select :open @14:27:09.66 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:27:09.68 SS.reconfigure SSinhale.with maxSpeed: 39 @14:27:09.73 SS.seek 25ml @14:27:35.16 Vacuum too high -- waiting up to 40s @14:27:36.00 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:27:36.02 SS.reconfigure SSinhale.with maxSpeed: 35 @14:27:36.07 SS.seek 25ml @14:27:58.39 Stroke completed -- waiting for pressure to equalize ... @14:28:01.51 Intake.select :close @14:28:01.67 SSV.dial :exhaust @14:28:02.48 Exhaust.select :open @14:28:02.59 SS.reconfigure SSexhale @14:28:02.64 SS.seek 0ml @14:28:11.86 Exhaust.select :close @14:28:11.98 SSV.dial CBV @14:28:12.01
CBV.dial SSV @14:28:12.82 Allowing 130s for 25.0ml of remaining 900.0ml @14:28:12.83 Intake.select :open @14:28:15.01 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:28:15.02 SS.reconfigure SSinhale.with maxSpeed: 35 @14:28:15.08 SS.seek 25ml @14:29:05.31 Stroke completed -- waiting for pressure to equalize ... @14:29:08.55 Intake.select :close @14:29:08.71 SSV.dial :exhaust @14:29:09.50 Exhaust.select :open @14:29:09.61 SS.reconfigure SSexhale @14:29:09.66 SS.seek 0ml @14:29:18.58 Exhaust.select :close @14:29:18.70 SSV.dial CBV @14:29:18.73
CBV.dial SSV @14:29:19.53 Allowing 130s for 25.0ml of remaining 875.0ml @14:29:19.54 Intake.select :open @14:29:21.72 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:29:21.73 SS.reconfigure SSinhale.with maxSpeed: 35 @14:29:21.78 SS.seek 25ml @14:30:12.31 Stroke completed -- waiting for pressure to equalize ... @14:30:15.52 Intake.select :close @14:30:15.68 SSV.dial :exhaust @14:30:16.48 Exhaust.select :open @14:30:16.59 SS.reconfigure SSexhale @14:30:16.65 SS.seek 0ml @14:30:26.02 Exhaust.select :close @14:30:26.14 SSV.dial CBV @14:30:26.17
CBV.dial SSV @14:30:26.98 Allowing 130s for 25.0ml of remaining 850.0ml @14:30:26.99 Intake.select :open @14:30:29.16 Intake @34.4psia, vacuum from 20.0 to 24.0psi @14:30:29.18 SS.reconfigure SSinhale.with maxSpeed: 35 @14:30:29.23 SS.seek 25ml @14:31:16.73 Vacuum too high -- waiting up to 40s @14:31:17.31 Intake @32.4psia, vacuum from 20.0 to 24.0psi @14:31:17.33 SS.reconfigure SSinhale.with maxSpeed: 32 @14:31:17.38 SS.seek 25ml @14:31:20.83 Stroke completed -- waiting for pressure to equalize ... @14:31:23.40 Intake.select :close @14:31:23.56 SSV.dial :exhaust @14:31:24.34 Exhaust.select :open @14:31:24.46 SS.reconfigure SSexhale @14:31:24.51 SS.seek 0ml @14:31:33.75 Exhaust.select :close @14:31:33.87 SSV.dial CBV @14:31:33.90
CBV.dial SSV @14:31:34.71 Allowing 130s for 25.0ml of remaining 825.0ml @14:31:34.72 Intake.select :open @14:31:36.89 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:31:36.91 SS.reconfigure SSinhale.with maxSpeed: 32 @14:31:36.96 SS.seek 25ml @14:32:31.78 Stroke completed -- waiting for pressure to equalize ... @14:32:34.90 Intake.select :close @14:32:35.06 SSV.dial :exhaust @14:32:35.85 Exhaust.select :open @14:32:35.96 SS.reconfigure SSexhale @14:32:36.01 SS.seek 0ml @14:32:44.81 Exhaust.select :close @14:32:44.93 SSV.dial CBV @14:32:44.96
CBV.dial SSV @14:32:45.78 Allowing 130s for 25.0ml of remaining 800.0ml @14:32:45.79 Intake.select :open @14:32:47.97 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:32:47.98 SS.reconfigure SSinhale.with maxSpeed: 32 @14:32:48.03 SS.seek 25ml @14:33:43.39 Stroke completed -- waiting for pressure to equalize ... @14:33:46.63 Intake.select :close @14:33:46.79 SSV.dial :exhaust @14:33:47.57 Exhaust.select :open @14:33:47.68 SS.reconfigure SSexhale @14:33:47.73 SS.seek 0ml @14:33:56.78 Exhaust.select :close @14:33:56.90 SSV.dial CBV @14:33:56.93
CBV.dial SSV @14:33:57.73 Allowing 130s for 25.0ml of remaining 775.0ml @14:33:57.74 Intake.select :open @14:33:59.92 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:33:59.93 SS.reconfigure SSinhale.with maxSpeed: 32 @14:33:59.98 SS.seek 25ml @14:34:55.06 Stroke completed -- waiting for pressure to equalize ... @14:34:58.89 Intake.select :close @14:34:59.05 SSV.dial :exhaust @14:34:59.84 Exhaust.select :open @14:34:59.95 SS.reconfigure SSexhale @14:35:00.00 SS.seek 0ml @14:35:09.16 Exhaust.select :close @14:35:09.28 SSV.dial CBV @14:35:09.31
CBV.dial SSV @14:35:10.14 Allowing 130s for 25.0ml of remaining 750.0ml @14:35:10.15 Intake.select :open @14:35:12.32 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:35:12.34 SS.reconfigure SSinhale.with maxSpeed: 32 @14:35:12.39 SS.seek 25ml @14:36:06.44 Vacuum too high -- waiting up to 40s @14:36:07.52 Intake @32.4psia, vacuum from 20.0 to 24.0psi @14:36:07.54 SS.reconfigure SSinhale.with maxSpeed: 29 @14:36:07.59 SS.seek 25ml @14:36:08.84 Stroke completed -- waiting for pressure to equalize ... @14:36:12.07 Intake.select :close @14:36:12.22 SSV.dial :exhaust @14:36:13.00 Exhaust.select :open @14:36:13.12 SS.reconfigure SSexhale @14:36:13.18 SS.seek 0ml @14:36:22.39 Exhaust.select :close @14:36:22.51 SSV.dial CBV @14:36:23.02
CBV.dial SSV @14:36:23.89 Allowing 130s for 25.0ml of remaining 725.0ml @14:36:23.90 Intake.select :open @14:36:26.08 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:36:26.09 SS.reconfigure SSinhale.with maxSpeed: 29 @14:36:26.14 SS.seek 25ml @14:37:26.58 Stroke completed -- waiting for pressure to equalize ... @14:37:30.45 Intake.select :close @14:37:30.61 SSV.dial :exhaust @14:37:31.40 Exhaust.select :open @14:37:31.51 SS.reconfigure SSexhale @14:37:31.56 SS.seek 0ml @14:37:40.75 Exhaust.select :close @14:37:40.87 SSV.dial CBV @14:37:40.90
CBV.dial SSV @14:37:41.70 Allowing 130s for 25.0ml of remaining 700.0ml @14:37:41.71 Intake.select :open @14:37:43.88 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:37:43.90 SS.reconfigure SSinhale.with maxSpeed: 29 @14:37:43.95 SS.seek 25ml @14:38:39.76 Vacuum too high -- waiting up to 40s @14:38:40.86 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:38:40.88 SS.reconfigure SSinhale.with maxSpeed: 26 @14:38:40.92 SS.seek 25ml @14:38:46.14 Stroke completed -- waiting for pressure to equalize ... @14:38:49.99 Intake.select :close @14:38:50.15 SSV.dial :exhaust @14:38:50.94 Exhaust.select :open @14:38:51.06 SS.reconfigure SSexhale @14:38:51.11 SS.seek 0ml @14:39:00.31 Exhaust.select :close @14:39:00.43 SSV.dial CBV @14:39:00.46
CBV.dial SSV @14:39:01.28 Allowing 130s for 25.0ml of remaining 675.0ml @14:39:01.29 Intake.select :open @14:39:03.46 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:39:03.48 SS.reconfigure SSinhale.with maxSpeed: 26 @14:39:03.53 SS.seek 25ml @14:40:10.95 Stroke completed -- waiting for pressure to equalize ... @14:40:15.39 Intake.select :close @14:40:15.55 SSV.dial :exhaust @14:40:16.35 Exhaust.select :open @14:40:16.46 SS.reconfigure SSexhale @14:40:16.52 SS.seek 0ml @14:40:25.74 Exhaust.select :close @14:40:25.86 SSV.dial CBV @14:40:25.89
CBV.dial SSV @14:40:26.70 Allowing 130s for 25.0ml of remaining 650.0ml @14:40:26.71 Intake.select :open @14:40:28.88 Intake @34.7psia, vacuum from 20.0 to 24.0psi @14:40:28.90 SS.reconfigure SSinhale.with maxSpeed: 26 @14:40:28.95 SS.seek 25ml @14:41:19.45 Vacuum too high -- waiting up to 40s @14:41:20.56 Intake @32.2psia, vacuum from 20.0 to 24.0psi @14:41:20.58 SS.reconfigure SSinhale.with maxSpeed: 23 @14:41:20.63 SS.seek 25ml @14:41:40.48 Stroke completed -- waiting for pressure to equalize ... @14:41:45.00 Intake.select :close @14:41:45.16 SSV.dial :exhaust @14:41:45.97 Exhaust.select :open @14:41:46.08 SS.reconfigure SSexhale @14:41:46.13 SS.seek 0ml @14:41:55.44 Exhaust.select :close @14:41:55.56 SSV.dial CBV @14:41:55.59
CBV.dial SSV @14:41:56.40 Allowing 130s for 25.0ml of remaining 625.0ml @14:41:56.41 Intake.select :open @14:41:58.59 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:41:58.60 SS.reconfigure SSinhale.with maxSpeed: 23 @14:41:58.65 SS.seek 25ml @14:43:15.00 Stroke completed -- waiting for pressure to equalize ... @14:43:18.84 Intake.select :close @14:43:19.00 SSV.dial :exhaust @14:43:19.78 Exhaust.select :open @14:43:19.90 SS.reconfigure SSexhale @14:43:19.95 SS.seek 0ml @14:43:29.08 Exhaust.select :close @14:43:29.20 SSV.dial CBV @14:43:29.23
CBV.dial SSV @14:43:30.03 Allowing 130s for 25.0ml of remaining 600.0ml @14:43:30.04 Intake.select :open @14:43:32.22 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:43:32.24 SS.reconfigure SSinhale.with maxSpeed: 23 @14:43:32.29 SS.seek 25ml @14:44:48.31 Stroke completed -- waiting for pressure to equalize ... @14:44:53.43 Intake.select :close @14:44:53.61 SSV.dial :exhaust @14:44:54.41 Exhaust.select :open @14:44:54.53 SS.reconfigure SSexhale @14:44:54.59 SS.seek 0ml @14:45:03.44 Exhaust.select :close @14:45:03.56 SSV.dial CBV @14:45:03.59
CBV.dial SSV @14:45:04.42 Allowing 130s for 25.0ml of remaining 575.0ml @14:45:04.43 Intake.select :open @14:45:06.60 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:45:06.62 SS.reconfigure SSinhale.with maxSpeed: 23 @14:45:06.67 SS.seek 25ml @14:46:23.09 Stroke completed -- waiting for pressure to equalize ... @14:46:28.71 Intake.select :close @14:46:28.87 SSV.dial :exhaust @14:46:29.67 Exhaust.select :open @14:46:29.78 SS.reconfigure SSexhale @14:46:29.83 SS.seek 0ml @14:46:38.91 Exhaust.select :close @14:46:39.03 SSV.dial CBV @14:46:39.06
CBV.dial SSV @14:46:39.89 Allowing 130s for 25.0ml of remaining 550.0ml @14:46:39.90 Intake.select :open @14:46:42.07 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:46:42.09 SS.reconfigure SSinhale.with maxSpeed: 23 @14:46:42.14 SS.seek 25ml @14:47:13.72 Vacuum too high -- waiting up to 40s @14:47:14.56 Intake @32.6psia, vacuum from 20.0 to 24.0psi @14:47:14.58 SS.reconfigure SSinhale.with maxSpeed: 21 @14:47:14.63 SS.seek 25ml @14:48:03.80 Stroke completed -- waiting for pressure to equalize ... @14:48:09.56 Intake.select :close @14:48:09.72 SSV.dial :exhaust @14:48:10.51 Exhaust.select :open @14:48:10.62 SS.reconfigure SSexhale @14:48:10.67 SS.seek 0ml @14:48:20.02 Exhaust.select :close @14:48:20.14 SSV.dial CBV @14:48:20.17
CBV.dial SSV @14:48:21.00 Allowing 130s for 25.0ml of remaining 525.0ml @14:48:21.01 Intake.select :open @14:48:23.18 Intake @35.1psia, vacuum from 20.0 to 24.0psi @14:48:23.20 SS.reconfigure SSinhale.with maxSpeed: 21 @14:48:23.25 SS.seek 25ml @14:48:55.94 Vacuum too high -- waiting up to 40s @14:48:56.76 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:48:56.78 SS.reconfigure SSinhale.with maxSpeed: 19 @14:48:56.83 SS.seek 25ml @14:49:53.42 Stroke completed -- waiting for pressure to equalize ... @14:49:59.13 Intake.select :close @14:49:59.28 SSV.dial :exhaust @14:50:00.06 Exhaust.select :open @14:50:00.18 SS.reconfigure SSexhale @14:50:00.23 SS.seek 0ml @14:50:09.30 Exhaust.select :close @14:50:09.42 SSV.dial CBV @14:50:09.45
CBV.dial SSV @14:50:10.28 Allowing 130s for 25.0ml of remaining 500.0ml @14:50:10.29 Intake.select :open @14:50:12.46 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:50:12.48 SS.reconfigure SSinhale.with maxSpeed: 19 @14:50:12.53 SS.seek 25ml @14:51:44.61 Stroke completed -- waiting for pressure to equalize ... @14:51:51.55 Intake.select :close @14:51:51.71 SSV.dial :exhaust @14:51:52.50 Exhaust.select :open @14:51:52.61 SS.reconfigure SSexhale @14:51:52.66 SS.seek 0ml @14:52:01.58 Exhaust.select :close @14:52:01.70 SSV.dial CBV @14:52:01.73
CBV.dial SSV @14:52:02.54 Allowing 130s for 25.0ml of remaining 475.0ml @14:52:02.55 Intake.select :open @14:52:04.73 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:52:04.74 SS.reconfigure SSinhale.with maxSpeed: 19 @14:52:04.79 SS.seek 25ml @14:53:37.38 Stroke completed -- waiting for pressure to equalize ... @14:53:44.42 Intake.select :close @14:53:44.58 SSV.dial :exhaust @14:53:45.37 Exhaust.select :open @14:53:45.48 SS.reconfigure SSexhale @14:53:45.54 SS.seek 0ml @14:53:54.41 Exhaust.select :close @14:53:54.53 SSV.dial CBV @14:53:54.56
CBV.dial SSV @14:53:55.37 Allowing 130s for 25.0ml of remaining 450.0ml @14:53:55.38 Intake.select :open @14:53:57.55 Intake @34.5psia, vacuum from 20.0 to 24.0psi @14:53:57.57 SS.reconfigure SSinhale.with maxSpeed: 19 @14:53:57.62 SS.seek 25ml @14:54:19.80 Vacuum too high -- waiting up to 40s @14:54:20.63 Intake @32.3psia, vacuum from 20.0 to 24.0psi @14:54:20.65 SS.reconfigure SSinhale.with maxSpeed: 17 @14:54:20.70 SS.seek 25ml @14:55:39.92 Stroke completed -- waiting for pressure to equalize ... @14:55:46.91 Intake.select :close @14:55:47.07 SSV.dial :exhaust @14:55:47.85 Exhaust.select :open @14:55:48.41 SS.reconfigure SSexhale @14:55:48.56 SS.seek 0ml @14:55:57.91 Exhaust.select :close @14:55:58.03 SSV.dial CBV @14:55:58.06
CBV.dial SSV @14:55:58.89 Allowing 130s for 25.0ml of remaining 425.0ml @14:55:58.90 Intake.select :open @14:56:01.07 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:56:01.09 SS.reconfigure SSinhale.with maxSpeed: 17 @14:56:01.15 SS.seek 25ml @14:57:44.14 Stroke completed -- waiting for pressure to equalize ... @14:57:51.82 Intake.select :close @14:57:51.98 SSV.dial :exhaust @14:57:52.78 Exhaust.select :open @14:57:52.89 SS.reconfigure SSexhale @14:57:52.94 SS.seek 0ml @14:58:02.19 Exhaust.select :close @14:58:02.31 SSV.dial CBV @14:58:02.34
CBV.dial SSV @14:58:03.17 Allowing 130s for 25.0ml of remaining 400.0ml @14:58:03.18 Intake.select :open @14:58:05.36 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:58:05.37 SS.reconfigure SSinhale.with maxSpeed: 17 @14:58:05.43 SS.seek 25ml @14:58:51.69 Vacuum too high -- waiting up to 40s @14:58:52.77 Intake @32.0psia, vacuum from 20.0 to 24.0psi @14:58:52.79 SS.reconfigure SSinhale.with maxSpeed: 15 @14:58:52.84 SS.seek 25ml @14:59:57.08 Stroke completed -- waiting for pressure to equalize ... @15:00:04.66 Intake.select :close @15:00:04.82 SSV.dial :exhaust @15:00:05.59 Exhaust.select :open @15:00:05.71 SS.reconfigure SSexhale @15:00:05.76 SS.seek 0ml @15:00:14.56 Exhaust.select :close @15:00:14.68 SSV.dial CBV @15:00:14.71
CBV.dial SSV @15:00:15.51 Allowing 130s for 25.0ml of remaining 375.0ml @15:00:15.52 Intake.select :open @15:00:17.69 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:00:17.71 SS.reconfigure SSinhale.with maxSpeed: 15 @15:00:17.76 SS.seek 25ml @15:01:57.13 Vacuum too high -- waiting up to 40s @15:01:58.76 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:01:58.78 SS.reconfigure SSinhale.with maxSpeed: 14 @15:01:58.83 SS.seek 25ml @15:02:18.05 Stroke completed -- waiting for pressure to equalize ... @15:02:25.53 SS.reconfigure SSconfig @15:02:25.58 SS.hold @15:02:25.71 Intake.select :close @15:02:25.87 ShallowSampler::Clogged in MAIN -- progress too slow after 1150.0ml @15:02:25.88 SSV.dial :exhaust @15:02:26.70 Exhaust.select :open @15:02:26.81 SS.reconfigure SSexhale @15:02:26.86 SS.seek 0ml @15:02:36.14 Exhaust.select :close @15:02:36.26 Sampled 1150.0ml @15:02:36.28 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @15:02:36.35 CSV.dial :waste @15:02:36.43 CS.seek 0ml @15:02:36.52 CSV.dial :air @15:02:36.56 SSV.dial CBV,avoiding: :exhaust @15:02:36.60
CBV.dial SSV @15:02:37.51 CS.seek 10ml @15:02:38.59
CTV.dial :air @15:02:39.38 delay 15 seconds @15:02:54.41 SSV.dial :spit,avoiding: :exhaust @15:02:55.78 CTV.dial CSV,avoiding: :intake @15:02:55.83
SS.seek 0ml @15:02:55.96 SSV.dial CBV,avoiding: :exhaust @15:03:01.77 CBV.dial between SSV and waste2 @15:03:02.20 CTV.dial CSV,avoiding: :air @15:03:02.23
CSV.dial CTV @15:03:02.74 CS.reconfigure CSpressurize @15:03:02.79 CS.seek 0ml @15:03:28.45 BubblePoint: Intake @68.9psia, Outlet @13.2psia @15:03:28.46 CBV.dial SSV @15:03:28.83 SS.reconfigure SSconfig @15:03:28.88 SS.seek 10ml @15:03:34.42 delay 15 seconds @15:03:49.56 Evacuated: Intake @34.3psia, Outlet @-0.2psia @15:03:49.58 SSV.dial :spit,avoiding: :exhaust @15:03:50.95 SS.reconfigure SSexhale @15:03:51.00 SS.seek 0ml @15:03:55.96 SS.reconfigure SSfast @15:03:56.02 SS.seek 5ml @15:03:58.95 SSV.dial CBV,avoiding: :exhaust @15:03:58.98
CBV.dial SSV @15:04:00.37 CTV.dial :air,avoiding: :intake @15:04:01.74 CSV.dial CTV @15:04:01.78
SS.seek 10ml @15:04:02.77 CSV.dial :air @15:04:03.25 CS.reconfigure CSfast @15:04:03.30 CS.seek 0ml @15:04:04.68
SSV.dial :spit,avoiding: :exhaust @15:04:05.26 CS.coast @15:04:06.07
SS.reconfigure SSexhale @15:04:06.12 SS.seek 0ml @15:04:11.43 SS.reconfigure SSfast @15:04:11.49 SS.seek 5ml @15:04:14.01 SSV.dial CBV,avoiding: :exhaust @15:04:14.04
CBV.dial SSV @15:04:16.47 SSV.dial :spit,avoiding: :exhaust @15:04:17.84 SS.reconfigure SSconfig @15:04:17.89 SS.seek 0ml @15:04:21.17 SS.coast @15:04:21.22 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @15:04:21.23 Intake.select :close @15:04:21.31 CSV.dial :waste @15:04:21.45 SSV.dial CBV,avoiding: :exhaust @15:04:21.49
CBV.dial SSV @15:04:22.16 CS.seek 0ml @15:04:22.23 CSV.dial :air @15:04:22.87
CTV.dial :air @15:04:22.88 delay 15 seconds @15:04:23.13 CS.seek 10ml @15:04:37.91
SSV.dial :spit,avoiding: :exhaust @15:04:39.28 CTV.dial CSV,avoiding: :intake @15:04:39.32
SS.reconfigure SSexhale @15:04:39.43 SS.seek 0ml @15:04:39.71 SSV.dial CBV,avoiding: :exhaust @15:04:47.18 CBV.dial between SSV and waste2 @15:04:47.65 CSV.dial between air and CTV @15:04:48.03 CS.seek 8.282ml @15:04:53.12 CTV.dial CSV,avoiding: :air @15:04:53.15
CSV.dial CTV @15:04:53.49 CS.reconfigure CSpressurize @15:04:53.55 CS.seek 0ml @15:05:15.36 CSV.dial :air @15:05:15.85 CS.reconfigure CSfast @15:05:15.90 CS.seek 10ml @15:05:39.61 CSV.dial between air and CTV @15:05:39.97 CS.seek 3.123ml @15:05:56.77 CTV.dial CSV,avoiding: :air @15:05:56.80
CSV.dial CTV @15:05:57.12 CS.reconfigure CSpressurize @15:05:57.17 CS.seek 0ml @15:06:05.08 BubblePoint: Intake @68.4psia, Outlet @12.8psia @15:06:05.09 CBV.dial SSV @15:06:05.44 SS.reconfigure SSconfig @15:06:05.50 SS.seek 10ml @15:06:10.87 delay 15 seconds @15:06:26.01 Evacuated: Intake @39.1psia, Outlet @14.1psia @15:06:26.02 SSV.dial :spit,avoiding: :exhaust @15:06:27.40 SS.reconfigure SSexhale @15:06:27.46 SS.seek 0ml @15:06:32.36 SS.reconfigure SSfast @15:06:32.41 SS.seek 5ml @15:06:35.09 SSV.dial CBV,avoiding: :exhaust @15:06:35.12
CBV.dial SSV @15:06:36.51 CTV.dial :air,avoiding: :intake @15:06:38.41 CSV.dial CTV @15:06:38.46
SS.seek 10ml @15:06:39.45 CSV.dial :air @15:06:39.93 CS.reconfigure CSfast @15:06:39.98 CS.seek 0ml @15:06:41.15
SSV.dial :spit,avoiding: :exhaust @15:06:41.54 CS.coast @15:06:42.54
SS.reconfigure SSexhale @15:06:42.59 SS.seek 0ml @15:06:47.57 SS.reconfigure SSfast @15:06:47.63 SS.seek 5ml @15:06:50.26 SSV.dial CBV,avoiding: :exhaust @15:06:50.29
CBV.dial SSV @15:06:52.68 SSV.dial :spit,avoiding: :exhaust @15:06:54.06 SS.reconfigure SSconfig @15:06:54.12 SS.seek 0ml @15:06:57.27 SS.coast @15:06:57.32 Lysing: Adding 1.5ml lysis @15:06:57.33 CBV.dial between CSV and waste1,avoiding: :DBV @15:06:58.88 CTV.dial CSV @15:07:00.24 CSV.dial CSR @15:07:01.07 Pulling 1ml of CSR.lysis @15:07:01.08 CSR.select :lysis @15:07:01.20 CS.reconfigure CSconfig @15:07:01.26 CS.seek 1ml @15:07:04.77 delay 5 seconds @15:07:09.79 CSR.select :air @15:07:09.98 CS.seek 6ml @15:07:23.74 delay 2 seconds @15:07:25.75 CSV.dial :waste1 @15:07:27.10 CS.reconfigure CSfast @15:07:27.15 CS.seek :empty @15:07:41.89 CSV.dial CSR @15:07:43.30 Pulling 1.5ml of CSR.lysis @15:07:43.31 CSR.select :lysis @15:07:43.43 CS.reconfigure CSconfig @15:07:43.49 CS.seek 1.5ml @15:07:48.43 delay 5 seconds @15:07:53.45 CSR.select :air @15:07:53.64 CS.seek 4.5ml @15:08:02.27 delay 2 seconds @15:08:04.28 CSV.dial CTV @15:08:04.77 CS.seek 1.275ml @15:08:13.93 Heating sh1 puck from tube 3 in Collection Clamp to 85C for 5:00 @15:08:13.96 CH.seek 85C,8:00 @15:10:57.69 CH.hold 5:00,85C @15:15:57.87 Collection Puck Heater at 85.1C after lysing @15:15:57.89 CS.seek 0ml @15:16:02.19 CTV.dial between spare and air,avoiding: :intake @15:16:03.28 CSV.dial :air @15:16:03.78 CS.seek 5ml @15:16:17.49 CSV.dial CTV @15:16:17.98 delay 2 seconds @15:16:20.01 CSV.dial :air @15:16:20.48 delay 2 seconds @15:16:22.51 CSV.dial CTV @15:16:23.00 delay 2 seconds @15:16:25.01 CSV.dial :air @15:16:25.50 delay 2 seconds @15:16:27.52 CS.seek 0ml @15:16:41.50 Recovering Lysate into Collection syringe @15:16:41.51 CSV.dial CBV @15:16:42.56 CBV.dial CSV @15:16:42.98 CS.seek 5ml @15:16:56.69 delay 5 seconds @15:17:01.71 CTV.dial :air @15:17:02.24 CS.seek 7ml @15:17:08.28 delay 5 seconds @15:17:13.30 CSV.dial :air2 @15:17:13.79 CS.seek 10ml @15:17:22.44 CSV.dial between air and CTV,avoiding: CBV @15:17:23.63 Lysing Complete @15:17:23.71 Storing sh1 puck in Collection Clamp to tube 2 @15:17:23.91 SC.reconfigure SCtight @15:17:23.96 SC.seek 2 @15:17:26.13 SC.reconfigure SCloose @15:17:26.36 Elbow.reconfigure TightElbow @15:17:26.41 Elbow.seek 2 @15:17:28.31 Forearm.reconfigure TightForearm @15:17:28.36 Forearm.seek 2 + 700 counts @15:17:29.41 SE.reconfigure SEpick @15:17:29.48 SE.seek beamBreak @15:17:32.40 SE.configure SEconfig @15:17:32.58 Elbow.reconfigure LimpElbow @15:17:32.63 Forearm.reconfigure LooseForearm @15:17:32.69 Forearm.seek 2 @15:17:32.98 Elbow.hold @15:17:33.05 Elbow.reconfigure TightElbow @15:17:33.16 SE.seek 1.07 Pucks below Top Plate @15:17:33.65 Forearm.force -20 @15:17:33.70 Forearm.seek 2 + 349 counts @15:17:34.03 Forearm.reconfigure TightForearm @15:17:34.08 Forearm.seek 2 + 700 counts @15:17:34.39 SE.seek 2.07 Pucks below Top Plate @15:17:35.14 Forearm.reconfigure LooseForearm @15:17:35.19 Forearm.seek :retract @15:17:36.62 Elbow.seek CC @15:17:38.42 Elbow.reconfigure LooseElbow @15:17:38.53 Forearm.seek CC @15:17:39.49 Hand.close @15:17:40.06 CC.seek :open @15:17:44.57 Forearm.seek :retract @15:17:45.57 Elbow.reconfigure TightElbow @15:17:45.62 Elbow.seek 2 @15:17:47.47 Elbow.reconfigure LimpElbow @15:17:47.53 Forearm.seek 2 @15:17:48.54 Elbow.hold @15:17:48.61 Elbow.reconfigure TightElbow @15:17:48.66 Hand.open @15:17:49.45 Forearm.force -20 @15:17:49.49 Forearm.seek 2 + 350 counts @15:17:49.82 Forearm.reconfigure TightForearm @15:17:49.87 Forearm.seek 2 + 700 counts @15:17:50.26 SE.seek :down @15:17:51.43
Forearm.reconfigure LooseForearm @15:17:51.48 Forearm.seek :retract @15:17:52.93 SE.coast @15:17:52.97
SC.coast @15:17:53.01 Tranferring lysate to PRV @15:17:53.02 CSV.dial :air @15:17:53.36 CS.seek 10ml @15:17:53.43 CSV.dial between air and CTV @15:17:53.78 AIV.dial :bypass @15:17:53.97 Beginning samplePassC @15:17:53.98 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:17:54.08 Hand.open @15:17:54.46 Elbow.seek FlushPuck::Garage @15:17:56.43 Elbow.reconfigure LooseElbow @15:17:56.54 Forearm.seek :garage @15:17:57.56 Hand.close @15:17:58.31 Forearm.seek :retract @15:17:59.35 Elbow.reconfigure TightElbow @15:17:59.40 Elbow.seek CC @15:18:02.26 Elbow.reconfigure LooseElbow @15:18:02.37 Forearm.reconfigure TightForearm @15:18:02.42 Forearm.seek CC @15:18:03.71 CC.seek :closed @15:18:07.97 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.01 @15:18:08.05 Hand.open @15:18:08.75 Forearm.reconfigure LooseForearm @15:18:08.80 Forearm.seek :retract @15:18:09.80 AWV.select :air @15:18:09.81 AIV.dial :bypass @15:18:09.82 PSV.dial PSR @15:18:11.25 Pulling 1.5ml of PSR.flush @15:18:11.26 PSR.select :flush @15:18:11.39 PS.seek 1.5ml @15:18:27.47 delay 5 seconds @15:18:32.49 PSV.dial :waste1 @15:18:32.97 PS.seek 0ml @15:18:48.99 PSV.dial PSR @15:18:49.47 PSR.select :air @15:18:49.60 PS.seek 2.5ml @15:19:15.75 PRV.dial CBV @15:19:15.78
CBV.dial PRV @15:19:16.64 PSV.dial :mixloop @15:19:17.69 CTV.dial :air,avoiding: :intake @15:19:17.70 PTV.dial :PRVmixing @15:19:17.79 PS.seek 0ml @15:19:43.93 PSV.dial :air @15:19:44.39 PS.seek 2.5ml @15:20:10.59 PSV.dial :mixloop @15:20:11.08 PS.seek 0ml @15:20:37.21 PSV.dial :air @15:20:37.68 PS.seek 2.5ml @15:21:03.81 PSV.dial :mixloop @15:21:04.29 PS.seek 0ml @15:21:30.41 Garaging FlushPuck in Collection Clamp @15:21:30.49 Elbow.reconfigure TightElbow @15:21:30.54 Elbow.seek CC @15:21:30.76 Elbow.reconfigure LooseElbow @15:21:30.87 Forearm.seek CC @15:21:31.85 Hand.close @15:21:32.44 CC.seek :open @15:21:36.82 Forearm.seek :retract @15:21:37.84 Elbow.reconfigure TightElbow @15:21:37.89 Elbow.seek :garage @15:21:41.05 Elbow.reconfigure LooseElbow @15:21:41.16 Forearm.reconfigure TightForearm @15:21:41.20 Forearm.seek :garage @15:21:42.38 Hand.open @15:21:43.13 Forearm.reconfigure LooseForearm @15:21:43.18 Forearm.seek :retract @15:21:44.22 Connecting PS to CS to transfer sample @15:21:44.23 CBV.dial AIV @15:21:44.24 AIV.dial :bypass @15:21:44.25 PSV.dial :mixloop @15:21:44.27 PTV.dial :PRVmixing @15:21:44.28 PRV.dial AIV @15:21:44.29 CTV.dial :air,avoiding: :intake @15:21:44.31 Loading lyfil puck from tube 3 into Collection Clamp @15:21:44.57 SC.reconfigure SCtight @15:21:44.62 SC.seek 3 @15:21:46.74 SC.reconfigure SCloose @15:21:46.96 Elbow.reconfigure TightElbow @15:21:47.01 Elbow.seek 3 @15:21:49.72 Forearm.reconfigure TightForearm @15:21:49.77 Forearm.seek 3 + 700 counts @15:21:50.87 SE.reconfigure SEpick @15:21:50.94 SE.seek beamBreak @15:21:52.27 SE.configure SEconfig @15:21:52.44 Elbow.seek 3 @15:21:52.65 Forearm.seek 3 + 300 counts @15:21:52.84 Forearm.reconfigure LooseForearm @15:21:52.88 Hand.close @15:21:53.58 SE.seek :down @15:21:53.82
Forearm.seek :retract @15:21:54.67 SE.coast @15:21:54.77
Elbow.seek CC @15:21:56.44 Elbow.reconfigure LooseElbow @15:21:56.55 Forearm.reconfigure TightForearm @15:21:56.60 Forearm.seek CC @15:21:57.90 CC.seek :closed @15:22:02.16 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.05 @15:22:02.17 Hand.open @15:22:02.88 Forearm.reconfigure LooseForearm @15:22:02.93 Forearm.seek :retract @15:22:03.88 SC.coast @15:22:03.92 CTV.dial CSV,avoiding: :intake @15:22:05.30 CSV.dial CTV @15:22:05.62 CS.seek 5ml @15:22:19.47 delay 1:20 @15:23:39.50 CS.seek 2.5ml @15:23:46.91 delay 40 seconds @15:24:26.94 CS.seek 0ml @15:24:34.38 delay 1:00 @15:25:34.40 PSV.dial :air @15:25:34.87 PS.seek 2.5ml @15:26:00.97 PSV.dial :mixloop @15:26:01.45 delay 3 seconds @15:26:04.46 PSV.dial :air @15:26:04.93 delay 2 seconds @15:26:06.95 PSV.dial :mixloop @15:26:07.41 delay 3 seconds @15:26:10.42 PSV.dial :waste1 @15:26:11.76 PS.seek :empty @15:26:37.87 CBV.dial :waste1 @15:26:38.37 delay 5 seconds @15:26:43.39 CBV.dial AIV @15:26:43.88 delay 10 seconds @15:26:53.90 CBV.dial :waste1 @15:26:54.40 delay 10 seconds @15:27:04.42 CBV.dial AIV @15:27:04.92 delay 10 seconds @15:27:14.95 CTV.dial CSV,avoiding: :intake @15:27:14.96 CBV.dial between AIV and waste1 @15:27:15.32 CSV.dial :air @15:27:15.82 CS.seek 10ml @15:27:42.25 CSV.dial CTV @15:27:42.73 CS.seek 0ml @15:28:09.28 CBV.dial :waste1 @15:28:09.60 delay 10 seconds @15:28:19.63 CTV.dial :air,avoiding: :intake @15:28:21.61 Storing lyfil puck in Collection Clamp to tube 2 @15:28:21.79 SC.reconfigure SCtight @15:28:21.84 SC.seek 2 @15:28:23.96 SC.reconfigure SCloose @15:28:24.19 Elbow.reconfigure TightElbow @15:28:24.24 Elbow.seek 2 @15:28:26.14 Forearm.reconfigure TightForearm @15:28:26.19 Forearm.seek 2 + 700 counts @15:28:27.22 SE.reconfigure SEpick @15:28:27.29 SE.seek beamBreak @15:28:30.11 SE.configure SEconfig @15:28:30.30 Elbow.reconfigure LimpElbow @15:28:30.35 Forearm.reconfigure LooseForearm @15:28:30.40 Forearm.seek 2 @15:28:30.73 Elbow.hold @15:28:30.80 Elbow.reconfigure TightElbow @15:28:30.91 SE.seek 2.07 Pucks below Top Plate @15:28:31.40 Forearm.force -20 @15:28:31.44 Forearm.seek 2 + 321 counts @15:28:31.78 Forearm.reconfigure TightForearm @15:28:31.83 Forearm.seek 2 + 700 counts @15:28:32.21 SE.seek 3.07 Pucks below Top Plate @15:28:32.82 Forearm.reconfigure LooseForearm @15:28:32.87 Forearm.seek :retract @15:28:33.77 Elbow.seek CC @15:28:35.49 Elbow.reconfigure LooseElbow @15:28:35.60 Forearm.seek CC @15:28:36.54 Hand.close @15:28:37.20 CC.seek :open @15:28:41.74 Forearm.seek :retract @15:28:42.71 Elbow.reconfigure TightElbow @15:28:42.76 Elbow.seek 2 @15:28:44.64 Elbow.reconfigure LimpElbow @15:28:44.69 Forearm.seek 2 @15:28:45.71 Elbow.hold @15:28:45.78 Elbow.reconfigure TightElbow @15:28:45.83 Hand.open @15:28:46.60 Forearm.force -20 @15:28:46.64 Forearm.seek 2 + 342 counts @15:28:46.97 Forearm.reconfigure TightForearm @15:28:47.02 Forearm.seek 2 + 700 counts @15:28:47.48 SE.seek :down @15:28:48.62
Forearm.reconfigure LooseForearm @15:28:48.67 Forearm.seek :retract @15:28:49.99 SE.coast @15:28:50.03
SC.coast @15:28:50.07 PSV.dial :air @15:28:51.19 PS.seek 0.499ml @15:28:57.57 delay 3 seconds @15:29:00.58 PSV.dial :mixloop @15:29:01.06 PRV.dial :air @15:29:01.56 delay 3 seconds @15:29:04.58 PRV.dial CBV @15:29:05.09 CBV.dial AIV @15:29:05.59 PRV.dial between CBV and air @15:29:05.95 PRV.dial :air @15:29:06.35 PS.reconfigure PSslow @15:29:06.40 PS.seek 0.599ml @15:29:09.00 PRV.dial :waste2 @15:29:09.79 PS.reconfigure PSconfig @15:29:09.84 PS.seek :empty @15:29:16.82 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:29:16.90 Hand.open @15:29:17.33 Elbow.seek FlushPuck::Garage @15:29:19.29 Elbow.reconfigure LooseElbow @15:29:19.40 Forearm.seek :garage @15:29:20.39 Hand.close @15:29:21.20 Forearm.seek :retract @15:29:22.26 Elbow.reconfigure TightElbow @15:29:22.31 Elbow.seek CC @15:29:25.23 Elbow.reconfigure LooseElbow @15:29:25.34 Forearm.reconfigure TightForearm @15:29:25.39 Forearm.seek CC @15:29:26.65 CC.seek :closed @15:29:30.92 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.98 @15:29:30.99 Hand.open @15:29:31.64 Forearm.reconfigure LooseForearm @15:29:31.69 Forearm.seek :retract @15:29:32.75 Rinsing MixLoop with GuSCN @15:29:32.76 PSV.dial :mixloop,avoiding: PBV @15:29:32.77 PTV.dial :PRVmixing,avoiding: :puck @15:29:32.78 PRV.dial :spare1 @15:29:33.91 PS.seek 1.099ml @15:29:45.82 delay 2 seconds @15:29:47.83 PRV.dial :air @15:29:48.90 PS.seek 2.5ml @15:30:03.88 PSV.dial :waste1,avoiding: PBV @15:30:05.23 PS.seek :empty @15:30:31.47 Diluting Lysate in Syringe @15:30:31.50 PSV.dial :PTV,avoiding: PBV @15:30:33.14 PTV.dial :PRV,avoiding: :puck @15:30:33.90 CBV.dial :PRV @15:30:33.91 PRV.dial :air @15:30:33.92 delay 2 seconds @15:30:35.95 PRV.dial CBV @15:30:36.45 delay 2 seconds @15:30:38.48 PS.reconfigure PSslow1 @15:30:38.53 PS.seek 0.2ml @15:30:59.95 delay 5 seconds @15:31:04.97 PRV.dial :spare1 @15:31:05.73 PS.reconfigure PSconfig @15:31:05.78 PS.seek 1ml @15:31:14.67 delay 2 seconds @15:31:16.69 PRV.dial :air @15:31:18.31 PS.seek 2.5ml @15:31:34.29 delay 2 seconds @15:31:36.31 Parking diluted lysate in Mixing Coil @15:31:36.32 PSV.dial :mixloop @15:31:36.90 PTV.dial :PRVmixing,avoiding: :puck @15:31:37.67 PRV.dial :CBV @15:31:38.24 PS.seek 1.5ml @15:31:49.20 delay 2 seconds @15:31:51.22 PSV.dial :waste1,avoiding: PBV @15:31:52.57 PS.seek :empty @15:32:08.64 Beginning samplePassF @15:32:08.72 AWV.select :air @15:32:08.73 AIV.dial :bypass @15:32:08.74 PSV.dial PSR,avoiding: PBV @15:32:09.29 Pulling 1.5ml of PSR.flush @15:32:09.30 PSR.select :flush @15:32:09.43 PS.seek 1.5ml @15:32:25.51 delay 5 seconds @15:32:30.53 PSV.dial :waste1 @15:32:31.03 PS.seek 0ml @15:32:47.00 PSV.dial PSR @15:32:47.47 PSR.select :air @15:32:47.61 PS.seek 2.5ml @15:33:13.73 PRV.dial CBV @15:33:13.76
CBV.dial PRV @15:33:13.77 PSV.dial :PTV,via: :air @15:33:15.70 CTV.dial :air,avoiding: :intake @15:33:15.71 PTV.dial :PRV,avoiding: :puck @15:33:16.48 PS.seek 0ml @15:33:42.66 PSV.dial :air @15:33:43.42 PS.seek 2.5ml @15:34:09.57 PSV.dial :PTV @15:34:10.34 PS.seek 0ml @15:34:36.58 moving diluted lysate into CBV-AIV line @15:34:36.59 PSV.dial :air @15:34:37.35 PS.seek 2.5ml @15:35:03.48 delay 2 seconds @15:35:05.49 PSV.dial :mixloop @15:35:05.97 PTV.dial :PRVmixing,avoiding: :puck @15:35:06.75 PRV.dial CBV @15:35:06.83 PS.seek 1.8ml @15:35:14.73 delay 3 seconds @15:35:17.74 PRV.dial :waste2 @15:35:18.81 PS.seek :empty @15:35:37.92 Clearing PML for modified lysate @15:35:37.93 CTV.dial :air,avoiding: :intake @15:35:37.94 PRV.dial :waste2 @15:35:37.96 PTV.dial :PRV,avoiding: :puck @15:35:38.72 delay 2 seconds @15:35:40.74 PTV.dial :PRVmixing,avoiding: :puck @15:35:41.50 delay 2 seconds @15:35:43.51 PSV.dial :PML @15:35:43.53 delay 2 seconds @15:35:45.55 PRV.dial :air @15:35:46.33 delay 2 seconds @15:35:48.41 PS.seek 1.499ml @15:36:04.45 delay 5 seconds @15:36:09.48 PS.seek 2.5ml @15:36:20.54 PSV.dial :air @15:36:21.05 PS.seek 0ml @15:36:47.23 mixing lysate with speDil @15:36:47.24 PSV.dial :PML @15:36:47.70 CBV.dial :PRV @15:36:47.71 PRV.dial :air @15:36:47.72 delay 2 seconds @15:36:49.74 PRV.dial :speDil @15:36:50.25 delay 2 seconds @15:36:52.35 PS.reconfigure PSslow1 @15:36:52.40 PS.seek 0.225ml @15:37:16.11 delay 5 seconds @15:37:21.13 PRV.dial CBV @15:37:21.94 delay 2 seconds @15:37:24.02 PS.seek 0.475ml @15:37:49.80 delay 5 seconds @15:37:54.82 PRV.dial :air @15:37:55.36 delay 2 seconds @15:37:57.39 PS.seek 0.965ml @15:38:47.33 delay 10 seconds @15:38:57.35 PRV.dial CBV @15:38:57.83 CTV.dial :air,avoiding: :intake @15:38:57.84 PSV.dial :air @15:38:58.30 PS.reconfigure PSconfig @15:38:58.88 PS.seek 2.5ml @15:39:15.33 delay 3 seconds @15:39:18.34 PSV.dial PTV @15:39:19.11 PTV.dial :PRV,avoiding: :puck @15:39:19.89 PS.seek 0ml @15:39:46.01 PSV.dial :air @15:39:46.79 PS.seek 2.5ml @15:40:12.92 delay 3 seconds @15:40:15.94 PSV.dial PTV @15:40:16.70 PTV.dial :PRV,avoiding: :puck @15:40:16.72 PS.seek 0ml @15:40:42.89 Pushing modified lysate into AIV Injection Loop @15:40:42.90 CBV.dial AIV @15:40:42.91 AIV.dial :bypass @15:40:42.92 PRV.dial CBV @15:40:42.94 PSV.dial :air @15:40:43.70 PS.seek 2.5ml @15:41:09.92 delay 3 seconds @15:41:12.93 PSV.dial :PML @15:41:13.39 PTV.dial :PRVmixing,avoiding: :puck @15:41:14.23 PS.reconfigure PSslow1 @15:41:14.28 PS.seek 1.74ml @15:42:31.61 delay 5 seconds @15:42:36.63 AIV.dial :sample @15:42:37.23 AWV.select :waste @15:42:37.40 PS.reconfigure PSslow2 @15:42:37.45 PS.seek 1.22ml @15:44:25.68 delay 15 seconds @15:44:40.70 AIV.dial :bypass @15:44:41.29 PS.reconfigure PSconfig @15:44:41.34 PS.seek 0ml @15:44:54.48 PSV.dial :air @15:44:54.97 PS.seek 2.5ml @15:45:21.07 PSV.dial :PML @15:45:21.54 PS.seek 0ml @15:45:47.60 AIV.dial :coilToMFB @15:45:47.62 AWV.select :air @15:45:47.78 Extracting Nucleic Acid from AIV @15:45:47.81
Beginning samplePassD @15:45:47.85 SPE.hold 2:46:40,55C @15:45:47.95 ASV.dial :SPEdelivery @15:45:48.02
AWV.select :air @15:45:48.03 AIV.dial :bypass @15:45:48.05 PSV.dial PSR @15:45:48.34 delay 20 seconds @15:45:49.31
Pulling 1.5ml of PSR.killMFB @15:45:49.32 PSR.select :killMFB @15:45:49.45 PS.seek 1.5ml @15:46:05.43 delay 5 seconds @15:46:08.37 primeLysateINT @15:46:08.38 AWV.select :air @15:46:08.39 AIV.dial :coilToMFB @15:46:08.41 ATV.dial AIV @15:46:10.08 AS.reconfigure ASslow1 @15:46:10.13 AS.seek 0.299ml @15:46:10.46
PSV.dial :waste1 @15:46:10.97 PS.seek 0ml @15:46:27.04 PSV.dial PSR @15:46:27.54 PSR.select :air @15:46:27.67 PS.seek 2.5ml @15:46:53.74 PRV.dial CBV @15:46:53.77
CBV.dial PRV @15:46:53.78 PSV.dial :mixloop @15:46:54.87 CTV.dial :air,avoiding: :intake @15:46:54.88 PTV.dial :PRVmixing @15:46:54.90 PS.seek 0ml @15:47:10.75 delay 10 seconds @15:47:20.77 ATV.dial :air2 @15:47:21.07
PSV.dial PSR @15:47:21.60 AS.seek 0.329ml @15:47:22.22
Pulling 1.5ml of PSR.flush @15:47:22.23 PSR.select :flush @15:47:22.36 PS.seek 1.5ml @15:47:27.89 ATV.dial :waste2 @15:47:28.55 AS.reconfigure ASnorm @15:47:28.60 AS.seek :empty @15:47:38.46
delay 5 seconds @15:47:43.48 PSV.dial :waste1 @15:47:44.10 PS.seek 0ml @15:47:45.61 Lysate primed! @15:47:45.62 AFV.select :holdingCoil @15:47:45.63 ATV.dial :air2 @15:47:46.24 AS.reconfigure ASslow1 @15:47:46.29 AS.seek 0.069ml @15:48:00.08
PSV.dial PSR @15:48:00.60 PSR.select :air @15:48:00.72 delay 3 seconds @15:48:00.75
PS.seek 2.5ml @15:48:03.74 ATV.dial :AIV @15:48:04.58 AS.seek 0.469ml @15:48:26.83 PRV.dial CBV @15:48:26.86
CBV.dial PRV @15:48:26.87 PSV.dial :mixloop @15:48:27.96 CTV.dial :air,avoiding: :intake @15:48:27.97 PTV.dial :PRVmixing @15:48:27.99 PS.seek 0ml @15:48:54.11 PSV.dial PSR @15:48:55.27 Pulling 1.5ml of PSR.flush @15:48:55.28 PSR.select :flush @15:48:55.41 PS.seek 1.5ml @15:49:11.46 delay 5 seconds @15:49:16.48 PSV.dial :waste1 @15:49:16.98 PS.seek 0ml @15:49:25.28 delay 5 seconds @15:49:30.37 Solid Phase Extraction Heater at 49C @15:49:30.38 ATV.dial ASV @15:49:30.76 ASV.dial :SPEdelivery @15:49:30.84 AS.seek 0.041ml @15:49:33.09
PSV.dial PSR @15:49:33.59 PSR.select :air @15:49:33.72 PS.seek 2.5ml @15:49:59.90 PRV.dial CBV @15:49:59.93
CBV.dial PRV @15:49:59.94 PSV.dial :mixloop @15:50:01.02 CTV.dial :air,avoiding: :intake @15:50:01.03 PTV.dial :PRVmixing @15:50:01.05 PS.seek 0ml @15:50:27.17 PSV.dial PSR @15:50:28.32 Pulling 1.5ml of PSR.flush @15:50:28.33 PSR.select :flush @15:50:28.46 PS.seek 1.5ml @15:50:44.62 delay 5 seconds @15:50:49.65 PSV.dial :waste1 @15:50:50.13 PS.seek 0ml @15:50:57.19 delay 10 seconds @15:51:06.11
PSV.dial PSR @15:51:06.60 PSR.select :air @15:51:06.73 PS.seek 2.5ml @15:51:07.21 rinseLysateLineINT @15:51:07.22 AFV.select :carrierSolution @15:51:07.32 delay 2 seconds @15:51:09.35 AS.reconfigure ASconfig @15:51:09.40 AS.seek 1ml @15:51:32.87 PRV.dial CBV @15:51:32.90
CBV.dial PRV @15:51:32.91 PSV.dial :mixloop @15:51:33.98 CTV.dial :air,avoiding: :intake @15:51:33.99 PTV.dial :PRVmixing @15:51:34.01 PS.reconfigure PSslow @15:51:34.06 PS.seek 1.2ml @15:52:00.81 PSV.dial :waste1 @15:52:02.18 PS.reconfigure PSconfig @15:52:02.23 PS.seek 0ml @15:52:04.80 delay 1 second @15:52:05.81 AFV.select :holdingCoil @15:52:05.93 delay 1 second @15:52:06.94 ATV.dial AIV @15:52:07.47 AIV.dial :coilToMFB @15:52:07.48 AWV.select :waste @15:52:07.59 AS.reconfigure ASslow0 @15:52:07.64 AS.seek 0ml @15:52:15.26
PSV.dial :air @15:52:16.33 PS.seek 2.5ml @15:52:42.48 PSV.dial :mixloop @15:52:42.95 PS.reconfigure PSslow @15:52:43.00 PS.seek 0ml @15:53:34.14 PSV.dial :air @15:53:35.14 PS.reconfigure PSconfig @15:53:35.19 PS.seek 2.5ml @15:53:48.53 ATV.dial :air2 @15:53:49.18 AS.reconfigure ASslow1 @15:53:49.23 AS.seek 0.05ml @15:53:59.54 ATV.dial :AIV @15:54:00.45 AS.seek 0.01ml @15:54:01.33
PSV.dial :mixloop @15:54:01.81 PS.reconfigure PSslow @15:54:01.86 PS.seek 0ml @15:54:08.80 ATV.dial :waste @15:54:10.42 AS.seek :empty @15:54:12.86 ethanol wash @15:54:12.87 ATV.dial :air2 @15:54:14.05 AS.reconfigure ASslow2 @15:54:14.10 AS.seek 0.009ml @15:54:24.39 delay 3 seconds @15:54:27.42 Pulling 0.4ml of Ethanol Wash @15:54:27.43 ATV.dial :ethanol @15:54:28.21 AS.reconfigure ASslow1 @15:54:28.26 AS.seek 0.409ml @15:54:52.96
Cleaning Collection Syringe @15:54:52.97 CSV.dial CSR @15:54:53.50 Pulling 4ml of CSR.flush @15:54:53.51 CSR.select :flush @15:54:53.64 CS.seek 4ml @15:55:04.91 delay 5 seconds @15:55:09.93 CSR.select :air @15:55:10.07 CS.seek 10ml @15:55:26.38 CSV.dial CTV @15:55:26.41
CTV.dial CSV @15:55:27.86 CBV.dial :waste1 @15:55:28.36 CS.seek 0ml @15:55:48.95 delay 3 seconds @15:55:51.97 ATV.dial :air1 @15:55:52.36 delay 3 seconds @15:55:54.91
CSV.dial CSR @15:55:55.51 Pulling 4ml of CSR.flush @15:55:55.54 AS.seek 0.434ml @15:55:55.55
CSR.select :flush @15:55:55.73 CS.seek 4ml @15:56:00.88 delay 3 seconds @15:56:03.90 ATV.dial :ethanol @15:56:04.51 AS.seek 0.419ml @15:56:06.94
delay 5 seconds @15:56:07.83 delay 3 seconds @15:56:10.85 ATV.dial ASV @15:56:11.96
CSR.select :air @15:56:12.12 AS.seek 0.092ml @15:56:12.18
CS.seek 10ml @15:56:28.50 CSV.dial CTV @15:56:28.53
CTV.dial CSV @15:56:29.02 CBV.dial :waste1 @15:56:29.04 CS.seek 0ml @15:56:55.66 Garaging FlushPuck in Collection Clamp @15:56:55.75 Elbow.reconfigure TightElbow @15:56:55.79 Elbow.seek CC @15:56:56.10 Elbow.reconfigure LooseElbow @15:56:56.21 Forearm.seek CC @15:56:57.20 Hand.close @15:56:57.86 CC.seek :open @15:57:02.23 Forearm.seek :retract @15:57:03.24 Elbow.reconfigure TightElbow @15:57:03.29 Elbow.seek :garage @15:57:06.37 Elbow.reconfigure LooseElbow @15:57:06.48 Forearm.reconfigure TightForearm @15:57:06.52 Forearm.seek :garage @15:57:07.68 Hand.open @15:57:08.98 Forearm.reconfigure LooseForearm @15:57:09.03 Forearm.seek :retract @15:57:10.04 Whole Cell Archive sampling 1500ml @15:57:10.06 Loading wcr puck from tube 3 into Collection Clamp @15:57:10.33 SC.reconfigure SCtight @15:57:10.38 SC.seek 3 @15:57:12.55 SC.reconfigure SCloose @15:57:12.79 Elbow.reconfigure TightElbow @15:57:12.84 Elbow.seek 3 @15:57:14.96 Forearm.reconfigure TightForearm @15:57:15.02 Forearm.seek 3 + 700 counts @15:57:16.06 SE.reconfigure SEpick @15:57:16.14 SE.seek beamBreak @15:57:17.59 SE.configure SEconfig @15:57:17.76 Elbow.seek 3 @15:57:17.98 Forearm.seek 3 + 300 counts @15:57:18.11 delay 3 seconds @15:57:18.19 Forearm.reconfigure LooseForearm @15:57:18.24 Hand.close @15:57:18.88 SE.seek :down @15:57:19.12 Forearm.seek :retract @15:57:20.11 Elbow.seek CC @15:57:20.18 SE.coast @15:57:21.12 ATV.dial :waste2 @15:57:21.73 Elbow.reconfigure LooseElbow @15:57:21.85 Forearm.reconfigure TightForearm @15:57:21.90 Forearm.seek CC @15:57:21.97 AS.reconfigure ASconfig @15:57:22.03 AS.seek :empty @15:57:23.21 CC.seek :closed @15:57:27.47 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.03 @15:57:27.48 Hand.open @15:57:27.66 ATV.dial :air2 @15:57:28.05 AS.reconfigure ASslow1 @15:57:28.13 AS.seek 1ml @15:57:28.27 Forearm.reconfigure LooseForearm @15:57:28.32 Forearm.seek :retract @15:57:29.30 SC.coast @15:57:29.34 collectSample 1500ml w/bubble point of 55psi @15:57:29.36 Priming 7ml @15:57:29.37 Intake.select :close @15:57:29.38 Exhaust.select :close @15:57:29.40 SS.hold @15:57:29.63 Calibrating pressure sensors @15:57:29.64 CTV.dial :air @15:57:31.01 SSV.dial :spit,avoiding: :exhaust @15:57:31.03 SS.reconfigure SSfast @15:57:31.65 SS.seek 5ml @15:57:34.29 SSV.dial CBV @15:57:34.32 CBV.dial SSV @15:57:35.80 delay 3.8 seconds @15:57:39.76 SSV.dial :spit @15:57:41.16 SS.seek 0ml @15:57:43.82 SSV.dial CBV @15:57:45.20 CTV.dial :intake @15:57:45.99 SSV.dial CBV @15:57:46.02 CBV.dial SSV @15:57:46.05 Allowing 40s for 7.0ml of remaining 7.0ml @15:57:46.06 Intake.select :open @15:57:48.24 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:57:48.26 SS.reconfigure SSinhale.with maxSpeed: 150 @15:57:48.31 SS.seek 7ml @15:57:52.02 Vacuum too high -- waiting up to 40s @15:57:54.15 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:57:54.17 SS.reconfigure SSinhale.with maxSpeed: 135 @15:57:54.23 SS.seek 7ml @15:57:57.04 Stroke completed -- waiting for pressure to equalize ... @15:58:01.56 Intake.select :close @15:58:01.72 SSV.dial :exhaust @15:58:02.52 Exhaust.select :open @15:58:02.63 SS.reconfigure SSexhale @15:58:02.68 SS.seek 0ml @15:58:06.58 Exhaust.select :close @15:58:06.70 Sampling 1500.0ml @15:58:06.71 Intake.select :close @15:58:06.73 Exhaust.select :close @15:58:06.74 SS.reconfigure SSconfig @15:58:06.79 SS.hold @15:58:07.02 CTV.dial :intake @15:58:07.04 SSV.dial CBV @15:58:07.07 CBV.dial SSV @15:58:07.93 Allowing 130s for 25.0ml of remaining 1500.0ml @15:58:07.94 Intake.select :open @15:58:10.13 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:58:10.15 SS.reconfigure SSinhale.with maxSpeed: 135 @15:58:10.20 SS.seek 25ml @15:58:13.85 Vacuum too high -- waiting up to 40s @15:58:15.72 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:58:15.74 SS.reconfigure SSinhale.with maxSpeed: 122 @15:58:15.79 SS.seek 25ml @15:58:18.36 Vacuum too high -- waiting up to 40s @15:58:19.78 Intake @33.2psia, vacuum from 20.0 to 24.0psi @15:58:19.80 SS.reconfigure SSinhale.with maxSpeed: 110 @15:58:19.85 SS.seek 25ml @15:58:22.61 Vacuum too high -- waiting up to 40s @15:58:24.23 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:58:24.25 SS.reconfigure SSinhale.with maxSpeed: 99 @15:58:24.31 SS.seek 25ml @15:58:27.11 Vacuum too high -- waiting up to 40s @15:58:28.47 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:58:28.49 SS.reconfigure SSinhale.with maxSpeed: 89 @15:58:28.54 SS.seek 25ml @15:58:31.60 Vacuum too high -- waiting up to 40s @15:58:33.24 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:58:33.26 SS.reconfigure SSinhale.with maxSpeed: 80 @15:58:33.31 SS.seek 25ml @15:58:36.29 Vacuum too high -- waiting up to 40s @15:58:37.40 Intake @32.5psia, vacuum from 20.0 to 24.0psi @15:58:37.42 SS.reconfigure SSinhale.with maxSpeed: 72 @15:58:37.47 SS.seek 25ml @15:58:42.53 Vacuum too high -- waiting up to 40s @15:58:44.14 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:58:44.16 SS.reconfigure SSinhale.with maxSpeed: 65 @15:58:44.21 SS.seek 25ml @15:58:45.83 Stroke completed -- waiting for pressure to equalize ... @15:58:48.45 Intake.select :close @15:58:48.61 SSV.dial :exhaust @15:58:49.42 Exhaust.select :open @15:58:49.53 SS.reconfigure SSexhale @15:58:49.58 SS.seek 0ml @15:58:58.66 Exhaust.select :close @15:58:58.78 SSV.dial CBV @15:58:58.81 CBV.dial SSV @15:58:59.65 Allowing 130s for 25.0ml of remaining 1475.0ml @15:58:59.66 Intake.select :open @15:59:01.84 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:59:01.86 SS.reconfigure SSinhale.with maxSpeed: 65 @15:59:01.91 SS.seek 25ml @15:59:09.85 Vacuum too high -- waiting up to 40s @15:59:11.04 Intake @33.2psia, vacuum from 20.0 to 24.0psi @15:59:11.06 SS.reconfigure SSinhale.with maxSpeed: 59 @15:59:11.11 SS.seek 25ml @15:59:21.58 Vacuum too high -- waiting up to 40s @15:59:22.69 Intake @33.2psia, vacuum from 20.0 to 24.0psi @15:59:22.71 SS.reconfigure SSinhale.with maxSpeed: 53 @15:59:22.76 SS.seek 25ml @15:59:37.09 Stroke completed -- waiting for pressure to equalize ... @15:59:39.68 Intake.select :close @15:59:39.85 SSV.dial :exhaust @15:59:40.67 Exhaust.select :open @15:59:40.78 SS.reconfigure SSexhale @15:59:40.84 SS.seek 0ml @15:59:49.91 Exhaust.select :close @15:59:50.03 SSV.dial CBV @15:59:50.06 CBV.dial SSV @15:59:50.89 Allowing 130s for 25.0ml of remaining 1450.0ml @15:59:50.90 Intake.select :open @15:59:53.09 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:59:53.11 SS.reconfigure SSinhale.with maxSpeed: 53 @15:59:53.16 SS.seek 25ml @16:00:19.67 Vacuum too high -- waiting up to 40s @16:00:20.52 Intake @32.5psia, vacuum from 20.0 to 24.0psi @16:00:20.54 SS.reconfigure SSinhale.with maxSpeed: 48 @16:00:20.59 SS.seek 25ml @16:00:28.83 Stroke completed -- waiting for pressure to equalize ... @16:00:31.44 Intake.select :close @16:00:31.61 SSV.dial :exhaust @16:00:32.42 Exhaust.select :open @16:00:32.53 SS.reconfigure SSexhale @16:00:32.58 SS.seek 0ml @16:00:41.66 Exhaust.select :close @16:00:41.78 SSV.dial CBV @16:00:41.81 CBV.dial SSV @16:00:42.65 Allowing 130s for 25.0ml of remaining 1425.0ml @16:00:42.66 Intake.select :open @16:00:44.85 Intake @33.6psia, vacuum from 20.0 to 24.0psi @16:00:44.87 SS.reconfigure SSinhale.with maxSpeed: 48 @16:00:44.92 SS.seek 25ml @16:00:49.81 delay 5 seconds @16:00:54.83 ATV.dial ASV @16:00:55.86 ASV.dial :SPEdelivery @16:00:55.88 AS.seek 0.02ml @16:01:21.81 Stroke completed -- waiting for pressure to equalize ... @16:01:24.41 Intake.select :close @16:01:24.58 SSV.dial :exhaust @16:01:25.39 Exhaust.select :open @16:01:25.50 SS.reconfigure SSexhale @16:01:26.12 SS.seek 0ml @16:01:34.92 Exhaust.select :close @16:01:35.05 SSV.dial CBV @16:01:35.07 CBV.dial SSV @16:01:35.90 Allowing 130s for 25.0ml of remaining 1400.0ml @16:01:35.91 Intake.select :open @16:01:38.10 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:01:38.12 SS.reconfigure SSinhale.with maxSpeed: 48 @16:01:38.17 SS.seek 25ml @16:02:15.64 Stroke completed -- waiting for pressure to equalize ... @16:02:18.24 Intake.select :close @16:02:18.40 SSV.dial :exhaust @16:02:19.23 Exhaust.select :open @16:02:19.34 SS.reconfigure SSexhale @16:02:19.40 SS.seek 0ml @16:02:28.67 Exhaust.select :close @16:02:28.79 SSV.dial CBV @16:02:28.82 CBV.dial SSV @16:02:29.64 Allowing 130s for 25.0ml of remaining 1375.0ml @16:02:29.65 Intake.select :open @16:02:31.83 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:02:31.85 SS.reconfigure SSinhale.with maxSpeed: 48 @16:02:31.90 SS.seek 25ml @16:03:09.27 Stroke completed -- waiting for pressure to equalize ... @16:03:11.90 Intake.select :close @16:03:12.06 SSV.dial :exhaust @16:03:12.87 Exhaust.select :open @16:03:12.98 SS.reconfigure SSexhale @16:03:13.03 SS.seek 0ml @16:03:21.73 Exhaust.select :close @16:03:21.85 SSV.dial CBV @16:03:21.88 CBV.dial SSV @16:03:22.71 Allowing 130s for 25.0ml of remaining 1350.0ml @16:03:22.72 Intake.select :open @16:03:24.90 Intake @33.4psia, vacuum from 20.0 to 24.0psi @16:03:24.92 SS.reconfigure SSinhale.with maxSpeed: 48 @16:03:24.97 SS.seek 25ml @16:04:02.41 Stroke completed -- waiting for pressure to equalize ... @16:04:05.65 Intake.select :close @16:04:05.82 SSV.dial :exhaust @16:04:06.64 Exhaust.select :open @16:04:06.75 SS.reconfigure SSexhale @16:04:06.80 SS.seek 0ml @16:04:13.21 delay 5 seconds @16:04:15.83 Exhaust.select :close @16:04:15.95 SSV.dial CBV @16:04:15.98 CBV.dial SSV @16:04:16.81 Allowing 130s for 25.0ml of remaining 1325.0ml @16:04:16.82 Intake.select :open @16:04:18.22 ATV.dial :air2 @16:04:19.00 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:04:19.02 SS.reconfigure SSinhale.with maxSpeed: 48 @16:04:19.07 SS.seek 25ml @16:04:19.23 AS.seek 0.52ml @16:04:56.03 Stroke completed -- waiting for pressure to equalize ... @16:05:00.47 Intake.select :close @16:05:00.63 SSV.dial :exhaust @16:05:01.46 Exhaust.select :open @16:05:01.58 SS.reconfigure SSexhale @16:05:01.64 SS.seek 0ml @16:05:10.70 Exhaust.select :close @16:05:10.82 SSV.dial CBV @16:05:10.85 CBV.dial SSV @16:05:11.70 Allowing 130s for 25.0ml of remaining 1300.0ml @16:05:11.71 Intake.select :open @16:05:13.90 Intake @33.0psia, vacuum from 20.0 to 24.0psi @16:05:13.92 SS.reconfigure SSinhale.with maxSpeed: 48 @16:05:13.97 SS.seek 25ml @16:05:51.23 Stroke completed -- waiting for pressure to equalize ... @16:05:54.48 Intake.select :close @16:05:54.65 SSV.dial :exhaust @16:05:55.46 Exhaust.select :open @16:05:55.57 SS.reconfigure SSexhale @16:05:55.62 SS.seek 0ml @16:06:00.07 delay 5 seconds @16:06:04.84 Exhaust.select :close @16:06:04.96 SSV.dial CBV @16:06:04.99 CBV.dial SSV @16:06:05.08 ATV.dial ASV @16:06:05.82 Allowing 130s for 25.0ml of remaining 1275.0ml @16:06:05.83 Intake.select :open @16:06:06.04 ASV.dial :wasteR @16:06:06.40 AS.seek 0.3ml @16:06:08.02 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:06:08.04 SS.reconfigure SSinhale.with maxSpeed: 48 @16:06:08.09 SS.seek 25ml @16:06:27.89 Vacuum too high -- waiting up to 40s @16:06:28.99 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:06:29.01 SS.reconfigure SSinhale.with maxSpeed: 43 @16:06:29.06 SS.seek 25ml @16:06:48.73 Stroke completed -- waiting for pressure to equalize ... @16:06:50.92 delay 20 seconds @16:06:51.96 Intake.select :close @16:06:52.12 SSV.dial :exhaust @16:06:52.93 Exhaust.select :open @16:06:53.04 SS.reconfigure SSexhale @16:06:53.10 SS.seek 0ml @16:07:02.19 Exhaust.select :close @16:07:02.31 SSV.dial CBV @16:07:02.34 CBV.dial SSV @16:07:03.17 Allowing 130s for 25.0ml of remaining 1250.0ml @16:07:03.18 Intake.select :open @16:07:05.37 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:07:05.39 SS.reconfigure SSinhale.with maxSpeed: 43 @16:07:05.44 SS.seek 25ml @16:07:10.95 AS.seek 0.07ml @16:07:46.36 Stroke completed -- waiting for pressure to equalize ... @16:07:49.59 Intake.select :close @16:07:49.76 SSV.dial :exhaust @16:07:51.15 Exhaust.select :open @16:07:51.26 SS.reconfigure SSexhale @16:07:51.31 SS.seek 0ml @16:07:57.47 delay 20 seconds @16:08:00.17 Exhaust.select :close @16:08:00.29 SSV.dial CBV @16:08:00.32 CBV.dial SSV @16:08:01.18 Allowing 130s for 25.0ml of remaining 1225.0ml @16:08:01.19 Intake.select :open @16:08:03.37 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:08:03.39 SS.reconfigure SSinhale.with maxSpeed: 43 @16:08:03.44 SS.seek 25ml @16:08:17.48 ATV.dial :waste2 @16:08:18.21 AS.reconfigure ASconfig @16:08:18.26 AS.seek :empty @16:08:22.66 Elution @16:08:22.67 ATV.dial :air1 @16:08:23.73 AS.reconfigure ASslow1 @16:08:23.78 AS.seek 0.049ml @16:08:34.19 delay 3 seconds @16:08:37.21 ATV.dial :ElutionWater @16:08:37.87 AS.seek 0.109ml @16:08:45.11 Stroke completed -- waiting for pressure to equalize ... @16:08:48.35 Intake.select :close @16:08:48.52 SSV.dial :exhaust @16:08:49.34 Exhaust.select :open @16:08:49.45 SS.reconfigure SSexhale @16:08:49.50 SS.seek 0ml @16:08:50.25 delay 3 seconds @16:08:53.27 ATV.dial ASV,via: :air2 @16:08:55.20 ASV.dial :SPEelution @16:08:55.76 AFV.select :carrierSolution @16:08:55.93 AS.reconfigure ASnorm @16:08:55.98 AS.seek 0.759ml @16:08:58.25 Exhaust.select :close @16:08:58.37 SSV.dial CBV @16:08:58.40 CBV.dial SSV @16:08:59.23 Allowing 130s for 25.0ml of remaining 1200.0ml @16:08:59.24 Intake.select :open @16:09:01.43 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:09:01.45 SS.reconfigure SSinhale.with maxSpeed: 43 @16:09:01.50 SS.seek 25ml @16:09:29.04 delay 3 seconds @16:09:32.07 AFV.select :holdingCoil @16:09:32.22 AS.reconfigure ASslow1 @16:09:32.27 AS.seek 0.474ml @16:09:43.09 Stroke completed -- waiting for pressure to equalize ... @16:09:46.94 Intake.select :close @16:09:47.10 SSV.dial :exhaust @16:09:47.90 Exhaust.select :open @16:09:48.01 SS.reconfigure SSexhale @16:09:48.06 SS.seek 0ml @16:09:57.08 Exhaust.select :close @16:09:57.20 SSV.dial CBV @16:09:57.23 CBV.dial SSV @16:09:58.07 Allowing 130s for 25.0ml of remaining 1175.0ml @16:09:58.08 Intake.select :open @16:10:00.27 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:10:00.29 SS.reconfigure SSinhale.with maxSpeed: 43 @16:10:00.34 SS.seek 25ml @16:10:29.86 delay 5 seconds @16:10:34.96 AS.reconfigure ASslow2 @16:10:35.01 AS.seek 0.439ml @16:10:41.66 Stroke completed -- waiting for pressure to equalize ... @16:10:45.47 Intake.select :close @16:10:45.64 SSV.dial :exhaust @16:10:46.45 Exhaust.select :open @16:10:46.56 SS.reconfigure SSexhale @16:10:46.62 SS.seek 0ml @16:10:55.64 Exhaust.select :close @16:10:55.76 SSV.dial CBV @16:10:55.79 CBV.dial SSV @16:10:56.62 Allowing 130s for 25.0ml of remaining 1150.0ml @16:10:56.63 Intake.select :open @16:10:58.81 Intake @33.6psia, vacuum from 20.0 to 24.0psi @16:10:58.83 SS.reconfigure SSinhale.with maxSpeed: 43 @16:10:58.88 SS.seek 25ml @16:11:10.44 delay 1:00 @16:11:40.25 Stroke completed -- waiting for pressure to equalize ... @16:11:44.77 Intake.select :close @16:11:44.93 SSV.dial :exhaust @16:11:45.75 Exhaust.select :open @16:11:45.86 SS.reconfigure SSexhale @16:11:45.92 SS.seek 0ml @16:11:54.67 Exhaust.select :close @16:11:54.79 SSV.dial CBV @16:11:54.82 CBV.dial SSV @16:11:55.65 Allowing 130s for 25.0ml of remaining 1125.0ml @16:11:55.66 Intake.select :open @16:11:57.85 Intake @33.6psia, vacuum from 20.0 to 24.0psi @16:11:57.87 SS.reconfigure SSinhale.with maxSpeed: 43 @16:11:57.92 SS.seek 25ml @16:12:10.47 pushing eluate to holding coil @16:12:10.54 AS.seek 0.279ml @16:12:36.02 Vacuum too high -- waiting up to 40s @16:12:37.61 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:12:37.63 SS.reconfigure SSinhale.with maxSpeed: 39 @16:12:37.68 SS.seek 25ml @16:12:41.77 Stroke completed -- waiting for pressure to equalize ... @16:12:45.01 Intake.select :close @16:12:45.18 SSV.dial :exhaust @16:12:45.99 Exhaust.select :open @16:12:46.10 SS.reconfigure SSexhale @16:12:46.15 SS.seek 0ml @16:12:55.13 Exhaust.select :close @16:12:55.25 SSV.dial CBV @16:12:55.28 CBV.dial SSV @16:12:56.12 Allowing 130s for 25.0ml of remaining 1100.0ml @16:12:56.13 Intake.select :open @16:12:58.32 Intake @33.6psia, vacuum from 20.0 to 24.0psi @16:12:58.34 SS.reconfigure SSinhale.with maxSpeed: 39 @16:12:58.39 SS.seek 25ml @16:13:44.08 Stroke completed -- waiting for pressure to equalize ... @16:13:47.91 Intake.select :close @16:13:48.07 SSV.dial :exhaust @16:13:48.89 Exhaust.select :open @16:13:49.00 SS.reconfigure SSexhale @16:13:49.05 SS.seek 0ml @16:13:57.92 Exhaust.select :close @16:13:58.04 SSV.dial CBV @16:13:58.07 CBV.dial SSV @16:13:58.89 Allowing 130s for 25.0ml of remaining 1075.0ml @16:13:58.90 Intake.select :open @16:14:01.09 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:14:01.11 SS.reconfigure SSinhale.with maxSpeed: 39 @16:14:01.16 SS.seek 25ml @16:14:46.84 Stroke completed -- waiting for pressure to equalize ... @16:14:51.33 Intake.select :close @16:14:51.50 SSV.dial :exhaust @16:14:51.62 delay 30 seconds @16:14:52.31 Exhaust.select :open @16:14:52.42 SS.reconfigure SSexhale @16:14:52.48 SS.seek 0ml @16:15:01.55 Exhaust.select :close @16:15:01.67 SSV.dial CBV @16:15:01.70 CBV.dial SSV @16:15:02.54 Allowing 130s for 25.0ml of remaining 1050.0ml @16:15:02.55 Intake.select :open @16:15:04.73 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:15:04.76 SS.reconfigure SSinhale.with maxSpeed: 39 @16:15:04.81 SS.seek 25ml @16:15:21.65 ATV.dial :waste,via: :air3 @16:15:23.24 AS.reconfigure ASconfig @16:15:23.29 AS.seek :empty @16:15:39.69 Parking Bleach in LysateSource line and SPEcolumn @16:15:39.70 ATV.dial :air3 @16:15:40.59 AS.reconfigure ASslow1 @16:15:40.64 AS.seek 0.029ml @16:15:47.00 ATV.dial :ElutionWater @16:15:47.59 AS.seek 0.059ml @16:15:50.03 Stroke completed -- waiting for pressure to equalize ... @16:15:53.88 ATV.dial :air3 @16:15:54.54 AS.seek 0.069ml @16:15:54.71 Intake.select :close @16:15:54.88 SSV.dial :exhaust @16:15:55.70 Exhaust.select :open @16:15:55.81 SS.reconfigure SSexhale @16:15:55.87 SS.seek 0ml @16:15:56.87 delay 2 seconds @16:15:58.88 ATV.dial :bleach @16:15:59.34 AS.seek 0.769ml @16:16:05.09 Exhaust.select :close @16:16:05.21 SSV.dial CBV @16:16:05.24 CBV.dial SSV @16:16:06.06 Allowing 130s for 25.0ml of remaining 1025.0ml @16:16:06.07 Intake.select :open @16:16:08.26 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:16:08.28 SS.reconfigure SSinhale.with maxSpeed: 39 @16:16:08.32 SS.seek 25ml @16:16:47.29 Vacuum too high -- waiting up to 40s @16:16:48.65 Intake @33.4psia, vacuum from 20.0 to 24.0psi @16:16:48.67 SS.reconfigure SSinhale.with maxSpeed: 35 @16:16:48.72 SS.seek 25ml @16:16:56.31 Stroke completed -- waiting for pressure to equalize ... @16:17:00.19 Intake.select :close @16:17:00.35 SSV.dial :exhaust @16:17:01.17 Exhaust.select :open @16:17:01.31 SS.reconfigure SSexhale @16:17:01.38 SS.seek 0ml @16:17:10.48 Exhaust.select :close @16:17:10.60 SSV.dial CBV @16:17:10.63 CBV.dial SSV @16:17:11.46 Allowing 130s for 25.0ml of remaining 1000.0ml @16:17:11.47 Intake.select :open @16:17:13.65 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:17:13.67 SS.reconfigure SSinhale.with maxSpeed: 35 @16:17:13.72 SS.seek 25ml @16:18:04.14 Stroke completed -- waiting for pressure to equalize ... @16:18:08.08 Intake.select :close @16:18:08.24 SSV.dial :exhaust @16:18:09.06 Exhaust.select :open @16:18:09.17 SS.reconfigure SSexhale @16:18:09.23 SS.seek 0ml @16:18:18.11 Exhaust.select :close @16:18:18.23 SSV.dial CBV @16:18:18.26 CBV.dial SSV @16:18:19.07 Allowing 130s for 25.0ml of remaining 975.0ml @16:18:19.08 Intake.select :open @16:18:20.38 delay 3 seconds @16:18:21.26 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:18:21.28 SS.reconfigure SSinhale.with maxSpeed: 35 @16:18:21.33 SS.seek 25ml @16:18:23.40 ATV.dial :air3 @16:18:23.94 delay 3 seconds @16:18:27.02 AS.seek 0.794ml @16:18:32.34 delay 3 seconds @16:18:35.36 ATV.dial :bleach @16:18:35.97 AS.seek 0.779ml @16:18:39.29 delay 3 seconds @16:18:42.31 ATV.dial :AIV @16:18:43.48 AS.reconfigure ASconfig @16:18:43.53 AS.seek 0.015ml @16:19:12.00 Stroke completed -- waiting for pressure to equalize ... @16:19:16.51 Intake.select :close @16:19:16.68 SSV.dial :exhaust @16:19:17.50 Exhaust.select :open @16:19:18.12 SS.reconfigure SSexhale @16:19:18.20 SS.seek 0ml @16:19:27.41 Exhaust.select :close @16:19:27.53 SSV.dial CBV @16:19:27.56 CBV.dial SSV @16:19:27.75 ATV.dial :waste @16:19:28.40 Allowing 130s for 25.0ml of remaining 950.0ml @16:19:28.41 Intake.select :open @16:19:29.15 AS.seek :empty @16:19:30.34 ATV.dial :air3 @16:19:30.60 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:19:30.62 SS.reconfigure SSinhale.with maxSpeed: 35 @16:19:30.67 SS.seek 25ml @16:19:31.00 AS.reconfigure ASslow1 @16:19:31.05 AS.seek 0.009ml @16:19:33.37 delay 2 seconds @16:19:35.38 ATV.dial :bleach @16:19:35.84 AS.seek 0.579ml @16:20:21.03 Stroke completed -- waiting for pressure to equalize ... @16:20:26.17 Intake.select :close @16:20:26.33 SSV.dial :exhaust @16:20:27.13 Exhaust.select :open @16:20:27.24 SS.reconfigure SSexhale @16:20:27.29 SS.seek 0ml @16:20:36.50 Exhaust.select :close @16:20:36.62 SSV.dial CBV @16:20:36.65 CBV.dial SSV @16:20:37.49 Allowing 130s for 25.0ml of remaining 925.0ml @16:20:37.50 Intake.select :open @16:20:39.69 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:20:39.71 SS.reconfigure SSinhale.with maxSpeed: 35 @16:20:39.76 SS.seek 25ml @16:21:21.30 Vacuum too high -- waiting up to 40s @16:21:22.66 Intake @33.2psia, vacuum from 20.0 to 24.0psi @16:21:22.69 SS.reconfigure SSinhale.with maxSpeed: 32 @16:21:22.74 SS.seek 25ml @16:21:30.74 delay 3 seconds @16:21:32.64 Stroke completed -- waiting for pressure to equalize ... @16:21:33.75 ATV.dial :air3 @16:21:34.29 delay 3 seconds @16:21:37.16 Intake.select :close @16:21:37.32 SSV.dial :exhaust @16:21:37.44 AS.seek 0.604ml @16:21:38.15 Exhaust.select :open @16:21:38.26 SS.reconfigure SSexhale @16:21:38.31 SS.seek 0ml @16:21:42.73 delay 3 seconds @16:21:45.75 ATV.dial :bleach @16:21:46.34 AS.seek 0.589ml @16:21:47.28 Exhaust.select :close @16:21:47.40 SSV.dial CBV @16:21:47.43 CBV.dial SSV @16:21:48.84 Allowing 130s for 25.0ml of remaining 900.0ml @16:21:48.85 Intake.select :open @16:21:49.63 delay 3 seconds @16:21:51.04 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:21:51.06 SS.reconfigure SSinhale.with maxSpeed: 32 @16:21:51.11 SS.seek 25ml @16:21:52.64 ATV.dial :ASV @16:21:53.63 ASV.dial :wasteR @16:21:54.19 AS.seek 0ml @16:22:46.45 Stroke completed -- waiting for pressure to equalize ... @16:22:51.86 Intake.select :close @16:22:52.03 SSV.dial :exhaust @16:22:52.84 Exhaust.select :open @16:22:52.95 SS.reconfigure SSexhale @16:22:53.01 SS.seek 0ml @16:23:02.17 Exhaust.select :close @16:23:02.29 SSV.dial CBV @16:23:02.32 CBV.dial SSV @16:23:03.15 Allowing 130s for 25.0ml of remaining 875.0ml @16:23:03.16 Intake.select :open @16:23:05.35 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:23:05.37 SS.reconfigure SSinhale.with maxSpeed: 32 @16:23:05.42 SS.seek 25ml @16:23:49.52 Vacuum too high -- waiting up to 40s @16:23:50.61 Intake @33.4psia, vacuum from 20.0 to 24.0psi @16:23:50.63 SS.reconfigure SSinhale.with maxSpeed: 29 @16:23:50.68 SS.seek 25ml @16:23:52.95 delay 1:00 @16:24:02.66 Stroke completed -- waiting for pressure to equalize ... @16:24:07.18 Intake.select :close @16:24:07.34 SSV.dial :exhaust @16:24:08.17 Exhaust.select :open @16:24:08.28 SS.reconfigure SSexhale @16:24:08.33 SS.seek 0ml @16:24:17.64 Exhaust.select :close @16:24:17.76 SSV.dial CBV @16:24:17.79 CBV.dial SSV @16:24:18.62 Allowing 130s for 25.0ml of remaining 850.0ml @16:24:18.63 Intake.select :open @16:24:20.82 Intake @33.2psia, vacuum from 20.0 to 24.0psi @16:24:20.84 SS.reconfigure SSinhale.with maxSpeed: 29 @16:24:20.89 SS.seek 25ml @16:24:52.97 AFV.select :carrierSolution @16:24:53.08 delay 2 seconds @16:24:55.11 AS.reconfigure ASconfig @16:24:55.16 AS.seek 1ml @16:25:21.41 Stroke completed -- waiting for pressure to equalize ... @16:25:27.13 Intake.select :close @16:25:27.29 SSV.dial :exhaust @16:25:28.10 Exhaust.select :open @16:25:28.21 SS.reconfigure SSexhale @16:25:28.27 SS.seek 0ml @16:25:37.44 Exhaust.select :close @16:25:37.56 SSV.dial CBV @16:25:37.59 CBV.dial SSV @16:25:38.43 Allowing 130s for 25.0ml of remaining 825.0ml @16:25:38.44 Intake.select :open @16:25:40.63 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:25:40.65 SS.reconfigure SSinhale.with maxSpeed: 29 @16:25:40.70 SS.seek 25ml @16:25:52.92 delay 1 second @16:25:53.93 AFV.select :holdingCoil @16:25:54.04 delay 1 second @16:25:55.05 ATV.dial ASV @16:25:55.07 ASV.dial :wasteR @16:25:55.09 AS.reconfigure ASslow1 @16:25:55.13 AS.seek 0ml @16:26:41.02 Stroke completed -- waiting for pressure to equalize ... @16:26:46.82 Intake.select :close @16:26:46.98 SSV.dial :exhaust @16:26:47.81 Exhaust.select :open @16:26:47.92 SS.reconfigure SSexhale @16:26:47.97 SS.seek 0ml @16:26:57.41 Exhaust.select :close @16:26:57.53 SSV.dial CBV @16:26:57.56 CBV.dial SSV @16:26:58.38 Allowing 130s for 25.0ml of remaining 800.0ml @16:26:58.39 Intake.select :open @16:27:00.57 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:27:00.59 SS.reconfigure SSinhale.with maxSpeed: 29 @16:27:00.64 SS.seek 25ml @16:27:59.85 Vacuum too high -- waiting up to 40s @16:28:01.22 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:28:01.24 SS.reconfigure SSinhale.with maxSpeed: 26 @16:28:01.29 SS.seek 25ml @16:28:03.03 Stroke completed -- waiting for pressure to equalize ... @16:28:07.57 Intake.select :close @16:28:07.73 SSV.dial :exhaust @16:28:08.54 Exhaust.select :open @16:28:08.66 SS.reconfigure SSexhale @16:28:08.71 SS.seek 0ml @16:28:18.06 Exhaust.select :close @16:28:18.18 SSV.dial CBV @16:28:18.21 CBV.dial SSV @16:28:19.04 Allowing 130s for 25.0ml of remaining 775.0ml @16:28:19.05 Intake.select :open @16:28:21.23 Intake @35.1psia, vacuum from 20.0 to 24.0psi @16:28:21.25 SS.reconfigure SSinhale.with maxSpeed: 26 @16:28:21.30 SS.seek 25ml @16:29:16.53 delay 20 seconds @16:29:28.83 Stroke completed -- waiting for pressure to equalize ... @16:29:33.97 Intake.select :close @16:29:34.13 SSV.dial :exhaust @16:29:34.96 Exhaust.select :open @16:29:35.08 SS.reconfigure SSexhale @16:29:35.13 SS.seek 0ml @16:29:37.09 ATV.dial :air3 @16:29:38.50 AS.seek 0.59ml @16:29:44.30 Exhaust.select :close @16:29:44.42 SSV.dial CBV @16:29:44.45 CBV.dial SSV @16:29:45.28 Allowing 130s for 25.0ml of remaining 750.0ml @16:29:45.29 Intake.select :open @16:29:47.48 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:29:47.50 SS.reconfigure SSinhale.with maxSpeed: 26 @16:29:47.55 SS.seek 25ml @16:30:54.70 Stroke completed -- waiting for pressure to equalize ... @16:31:00.47 Intake.select :close @16:31:00.63 SSV.dial :exhaust @16:31:01.45 Exhaust.select :open @16:31:01.56 SS.reconfigure SSexhale @16:31:01.61 SS.seek 0ml @16:31:10.70 Exhaust.select :close @16:31:10.82 SSV.dial CBV @16:31:10.85 CBV.dial SSV @16:31:11.67 Allowing 130s for 25.0ml of remaining 725.0ml @16:31:11.68 Intake.select :open @16:31:13.87 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:31:13.89 SS.reconfigure SSinhale.with maxSpeed: 26 @16:31:13.94 SS.seek 25ml @16:31:37.45 delay 3 seconds @16:31:40.47 ATV.dial :ASV @16:31:41.79 ASV.dial :wasteR @16:31:41.87 AS.seek 0.09ml @16:32:21.47 Stroke completed -- waiting for pressure to equalize ... @16:32:27.86 Intake.select :close @16:32:28.02 SSV.dial :exhaust @16:32:28.82 Exhaust.select :open @16:32:28.93 SS.reconfigure SSexhale @16:32:28.98 SS.seek 0ml @16:32:37.77 Exhaust.select :close @16:32:38.48 SSV.dial CBV @16:32:38.51 CBV.dial SSV @16:32:39.35 Allowing 130s for 25.0ml of remaining 700.0ml @16:32:39.36 Intake.select :open @16:32:41.55 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:32:41.57 SS.reconfigure SSinhale.with maxSpeed: 26 @16:32:41.62 SS.seek 25ml @16:33:22.70 delay 30 seconds @16:33:23.69 Vacuum too high -- waiting up to 40s @16:33:25.32 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:33:25.35 SS.reconfigure SSinhale.with maxSpeed: 23 @16:33:25.40 SS.seek 25ml @16:33:52.72 ATV.dial :extSrc @16:33:53.45 delay 3 seconds @16:33:54.19 Stroke completed -- waiting for pressure to equalize ... @16:33:56.47 ATV.dial :ElutionWater @16:33:57.63 AS.seek 0.12ml @16:33:59.98 Intake.select :close @16:34:00.15 SSV.dial :exhaust @16:34:00.96 Exhaust.select :open @16:34:01.07 SS.reconfigure SSexhale @16:34:01.13 SS.seek 0ml @16:34:03.92 ATV.dial :air3 @16:34:04.50 AS.seek 0.13ml @16:34:06.78 delay 2 seconds @16:34:08.79 ATV.dial :bleach @16:34:09.25 AS.seek 0.23ml @16:34:09.91 Exhaust.select :close @16:34:10.03 SSV.dial CBV @16:34:10.06 CBV.dial SSV @16:34:10.90 Allowing 130s for 25.0ml of remaining 675.0ml @16:34:10.91 Intake.select :open @16:34:13.09 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:34:13.11 SS.reconfigure SSinhale.with maxSpeed: 23 @16:34:13.16 SS.seek 25ml @16:34:29.65 delay 3 seconds @16:34:32.67 ATV.dial :air3 @16:34:33.20 delay 3 seconds @16:34:36.35 AS.seek 0.255ml @16:34:41.68 delay 3 seconds @16:34:44.70 ATV.dial :bleach @16:34:45.41 AS.seek 0.24ml @16:34:48.67 delay 3 seconds @16:34:51.68 ATV.dial :ASV @16:34:52.66 ASV.dial :SPEdelivery @16:34:53.03 AFV.select :carrierSolution @16:34:53.15 delay 2 seconds @16:34:55.18 AS.reconfigure ASconfig @16:34:55.23 AS.seek 0.5ml @16:35:10.51 delay 1 second @16:35:11.53 AFV.select :holdingCoil @16:35:11.69 AS.reconfigure ASslow1 @16:35:11.74 AS.seek 0.17ml @16:35:29.59 Stroke completed -- waiting for pressure to equalize ... @16:35:36.01 Intake.select :close @16:35:36.18 SSV.dial :exhaust @16:35:37.01 Exhaust.select :open @16:35:37.12 SS.reconfigure SSexhale @16:35:37.18 SS.seek 0ml @16:35:46.34 Exhaust.select :close @16:35:46.46 SSV.dial CBV @16:35:46.49 CBV.dial SSV @16:35:47.32 Allowing 130s for 25.0ml of remaining 650.0ml @16:35:47.33 Intake.select :open @16:35:49.52 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:35:49.54 SS.reconfigure SSinhale.with maxSpeed: 23 @16:35:49.59 SS.seek 25ml @16:36:18.40 SPE.hold 2:46:40,95C @16:36:18.46 delay 3:00 @16:37:05.64 Stroke completed -- waiting for pressure to equalize ... @16:37:12.07 Intake.select :close @16:37:12.24 SSV.dial :exhaust @16:37:13.09 Exhaust.select :open @16:37:13.20 SS.reconfigure SSexhale @16:37:13.25 SS.seek 0ml @16:37:22.45 Exhaust.select :close @16:37:22.57 SSV.dial CBV @16:37:22.60 CBV.dial SSV @16:37:23.45 Allowing 130s for 25.0ml of remaining 625.0ml @16:37:23.46 Intake.select :open @16:37:25.65 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:37:25.67 SS.reconfigure SSinhale.with maxSpeed: 23 @16:37:25.72 SS.seek 25ml @16:38:41.98 Stroke completed -- waiting for pressure to equalize ... @16:38:49.66 Intake.select :close @16:38:49.83 SSV.dial :exhaust @16:38:50.67 Exhaust.select :open @16:38:50.78 SS.reconfigure SSexhale @16:38:50.84 SS.seek 0ml @16:38:59.80 Exhaust.select :close @16:38:59.92 SSV.dial CBV @16:38:59.95 CBV.dial SSV @16:39:00.81 Allowing 130s for 25.0ml of remaining 600.0ml @16:39:00.82 Intake.select :open @16:39:03.01 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:39:03.03 SS.reconfigure SSinhale.with maxSpeed: 23 @16:39:03.08 SS.seek 25ml @16:39:18.49 AFV.select :carrierSolution @16:39:18.60 delay 2 seconds @16:39:20.63 AS.reconfigure ASconfig @16:39:20.68 AS.seek 1ml @16:39:54.44 Vacuum too high -- waiting up to 40s @16:39:57.37 Intake @35.3psia, vacuum from 20.0 to 24.0psi @16:39:57.77 SS.reconfigure SSinhale.with maxSpeed: 21 @16:39:58.44 SS.seek 25ml @16:40:08.67 delay 1 second @16:40:09.68 AFV.select :holdingCoil @16:40:09.80 delay 1 second @16:40:10.82 ASV.dial :wasteD @16:40:11.82 AS.seek 0ml @16:40:28.12 Stroke completed -- waiting for pressure to equalize ... @16:40:35.22 Intake.select :close @16:40:35.39 SSV.dial :exhaust @16:40:36.23 Exhaust.select :open @16:40:36.34 SS.reconfigure SSexhale @16:40:36.40 SS.seek 0ml @16:40:45.71 Exhaust.select :close @16:40:45.82 SSV.dial CBV @16:40:45.85 CBV.dial SSV @16:40:46.70 Allowing 130s for 25.0ml of remaining 575.0ml @16:40:46.71 Intake.select :open @16:40:48.89 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:40:48.91 SS.reconfigure SSinhale.with maxSpeed: 21 @16:40:48.96 SS.seek 25ml @16:41:09.61 Priming Eluate @16:41:09.62 AFV.select :carrierSolution @16:41:09.73 delay 2 seconds @16:41:11.76 AS.seek 0.5ml @16:41:40.79 delay 1 second @16:41:41.80 AFV.select :holdingCoil @16:41:41.91 ATV.dial ASV @16:41:41.92 ASV.dial :SPErecovery @16:41:42.50 Swish #1 of 2 @16:41:42.58 AS.reconfigure ASslow0 @16:41:42.63 AS.seek 0.6ml @16:41:53.10 AS.seek 0.5ml @16:42:03.47 Swish #2 of 2 @16:42:03.56 AS.seek 0.6ml @16:42:12.39 Stroke completed -- waiting for pressure to equalize ... @16:42:14.04 AS.seek 0.5ml @16:42:21.36 Intake.select :close @16:42:21.52 SSV.dial :exhaust @16:42:22.35 Exhaust.select :open @16:42:22.46 SS.reconfigure SSexhale @16:42:22.52 SS.seek 0ml @16:42:24.42 delay 3 seconds @16:42:27.51 AS.reconfigure ASslow1 @16:42:27.56 AS.seek 0.684ml @16:42:32.31 Exhaust.select :close @16:42:32.44 SSV.dial CBV @16:42:32.47 CBV.dial SSV @16:42:33.32 Allowing 130s for 25.0ml of remaining 550.0ml @16:42:33.33 Intake.select :open @16:42:35.51 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:42:35.53 SS.reconfigure SSinhale.with maxSpeed: 21 @16:42:35.58 SS.seek 25ml @16:43:04.84 delay 3 seconds @16:43:07.87 Eluate primed @16:43:07.88 ATV.dial :waste2 @16:43:08.47 AS.reconfigure ASnorm @16:43:08.53 AS.seek 0ml @16:43:43.07 Vacuum too high -- waiting up to 40s @16:43:43.36 SPE.coast @16:43:43.45 ASV.dial :SPErecovery @16:43:43.48 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0816h1150ml.pcr @16:43:43.50 Building PCR Slug from ASV with MM2 mix, PP4 primer @16:43:43.51 Priming with carrier @16:43:43.52 ATV.dial ARV @16:43:44.50 ARV.dial :carrier @16:43:44.87 AS.seek 0.5ml @16:43:45.12 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:43:45.14 SS.reconfigure SSinhale.with maxSpeed: 19 @16:43:45.19 SS.seek 25ml @16:44:02.67 Stroke completed -- waiting for pressure to equalize ... @16:44:10.37 Intake.select :close @16:44:10.44 AFV.select :carrierSolution @16:44:10.55 SSV.dial :exhaust @16:44:10.61 delay 2 seconds @16:44:11.37 Exhaust.select :open @16:44:11.48 SS.reconfigure SSexhale @16:44:11.53 SS.seek 0ml @16:44:12.63 AS.reconfigure ASconfig @16:44:12.68 AS.seek 1ml @16:44:20.75 Exhaust.select :close @16:44:20.87 SSV.dial CBV @16:44:20.90 CBV.dial SSV @16:44:21.74 Allowing 130s for 25.0ml of remaining 525.0ml @16:44:21.75 Intake.select :open @16:44:23.94 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:44:23.96 SS.reconfigure SSinhale.with maxSpeed: 19 @16:44:24.01 SS.seek 25ml @16:44:41.70 delay 1 second @16:44:42.71 AFV.select :holdingCoil @16:44:42.82 delay 1 second @16:44:43.84 ATV.dial :waste @16:44:45.25 AS.reconfigure ASnorm @16:44:45.30 AS.seek :empty @16:45:36.05 AFV.select :carrierSolution @16:45:36.17 delay 2 seconds @16:45:38.20 AS.reconfigure ASconfig @16:45:38.25 AS.seek 0.5ml @16:45:56.23 Stroke completed -- waiting for pressure to equalize ... @16:46:05.16 Intake.select :close @16:46:05.33 SSV.dial :exhaust @16:46:06.13 Exhaust.select :open @16:46:06.24 SS.reconfigure SSexhale @16:46:06.31 SS.seek 0ml @16:46:07.31 delay 1 second @16:46:08.32 AFV.select :holdingCoil @16:46:08.44 Building Slug @16:46:08.45 ATV.dial :ARV @16:46:10.06 delay 2 seconds @16:46:12.08 ARV.dial :air1 @16:46:12.47 delay 2 seconds @16:46:14.55 AS.reconfigure ASslow2 @16:46:14.61 AS.seek 0.506ml @16:46:15.49 Exhaust.select :close @16:46:15.60 SSV.dial CBV @16:46:15.63 CBV.dial SSV @16:46:16.45 Allowing 130s for 25.0ml of remaining 500.0ml @16:46:16.46 Intake.select :open @16:46:18.64 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:46:18.66 SS.reconfigure SSinhale.with maxSpeed: 19 @16:46:18.71 SS.seek 25ml @16:46:20.91 delay 2 seconds @16:46:22.93 ARV.dial :MM2 @16:46:24.33 delay 2 seconds @16:46:26.37 Adding MM2 @16:46:26.44 AS.seek 0.524ml @16:46:44.75 delay 25 seconds @16:47:09.77 ARV.dial :PP4 @16:47:10.16 Adding PP4 @16:47:10.18 delay 2 seconds @16:47:12.27 AS.seek 0.53ml @16:47:18.61 delay 10 seconds @16:47:28.63 ARV.dial :air3 @16:47:29.03 adding ARV air @16:47:29.04 delay 2 seconds @16:47:31.13 AS.seek 0.536ml @16:47:37.45 delay 5 seconds @16:47:42.47 ARV.dial :carrier @16:47:43.66 delay 7 seconds @16:47:50.75 AS.seek 0.594ml @16:47:50.89 Stroke completed -- waiting for pressure to equalize ... @16:48:01.11 Intake.select :close @16:48:01.28 SSV.dial :exhaust @16:48:02.09 Exhaust.select :open @16:48:02.20 SS.reconfigure SSexhale @16:48:02.25 SS.seek 0ml @16:48:11.76 Exhaust.select :close @16:48:11.88 SSV.dial CBV @16:48:11.91 CBV.dial SSV @16:48:12.74 Allowing 130s for 25.0ml of remaining 475.0ml @16:48:12.75 Intake.select :open @16:48:14.94 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:48:14.96 SS.reconfigure SSinhale.with maxSpeed: 19 @16:48:15.01 SS.seek 25ml @16:48:49.34 delay 5 seconds @16:48:54.36 ATV.dial :ASV @16:48:54.77 delay 2 seconds @16:48:56.86 AS.seek 0.6ml @16:49:03.02 delay 4 seconds @16:49:07.04 ATV.dial ARV @16:49:07.67 delay 2 seconds @16:49:09.69 ARV.dial :carrier @16:49:09.71 delay 2 seconds @16:49:09.86 Vacuum too high -- waiting up to 40s @16:49:11.84 AS.seek 0.65ml @16:49:12.35 Intake @35.2psia, vacuum from 20.0 to 24.0psi @16:49:12.37 SS.reconfigure SSinhale.with maxSpeed: 17 @16:49:12.42 SS.seek 25ml @16:49:54.95 Stroke completed -- waiting for pressure to equalize ... @16:50:02.38 delay 4 seconds @16:50:03.94 Intake.select :close @16:50:04.10 SSV.dial :exhaust @16:50:04.92 Exhaust.select :open @16:50:05.03 SS.reconfigure SSexhale @16:50:05.10 SS.seek 0ml @16:50:06.40 ATV.dial PCR @16:50:07.00 ASV.dial :waste1 @16:50:07.75 ARV.dial :air1 @16:50:08.23 Positioning Slug by 0.324ml @16:50:08.32 AS.seek 0.326ml @16:50:14.23 Exhaust.select :close @16:50:14.35 SSV.dial CBV @16:50:14.38 CBV.dial SSV @16:50:15.20 Allowing 130s for 25.0ml of remaining 450.0ml @16:50:15.21 Intake.select :open @16:50:17.40 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:50:17.42 SS.reconfigure SSinhale.with maxSpeed: 17 @16:50:17.47 SS.seek 25ml @16:52:00.31 Stroke completed -- waiting for pressure to equalize ... @16:52:09.80 Intake.select :close @16:52:09.97 SSV.dial :exhaust @16:52:10.78 Exhaust.select :open @16:52:10.90 SS.reconfigure SSexhale @16:52:10.95 SS.seek 0ml @16:52:20.34 Exhaust.select :close @16:52:20.46 SSV.dial CBV @16:52:20.49 CBV.dial SSV @16:52:21.32 Allowing 130s for 25.0ml of remaining 425.0ml @16:52:21.33 Intake.select :open @16:52:23.52 Intake @35.0psia, vacuum from 20.0 to 24.0psi @16:52:23.54 SS.reconfigure SSinhale.with maxSpeed: 17 @16:52:23.59 SS.seek 25ml @16:53:41.32 Vacuum too high -- waiting up to 40s @16:53:43.22 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:53:43.24 SS.reconfigure SSinhale.with maxSpeed: 15 @16:53:43.29 SS.seek 25ml @16:54:12.42 Stroke completed -- waiting for pressure to equalize ... @16:54:21.37 Intake.select :close @16:54:21.54 SSV.dial :exhaust @16:54:22.35 Exhaust.select :open @16:54:22.46 SS.reconfigure SSexhale @16:54:22.53 SS.seek 0ml @16:54:31.55 Exhaust.select :close @16:54:31.67 SSV.dial CBV @16:54:31.70 CBV.dial SSV @16:54:32.52 Allowing 130s for 25.0ml of remaining 400.0ml @16:54:32.53 Intake.select :open @16:54:34.71 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:54:34.73 SS.reconfigure SSinhale.with maxSpeed: 15 @16:54:34.78 SS.seek 25ml @16:55:34.60 delay 5 seconds @16:55:39.62 ATV.dial ARV @16:55:40.45 ASV.dial :PCRlock @16:55:40.80 delay 5 seconds @16:55:45.83 Running Tt1 Profile60C300 thermocycling @16:55:45.84 PCR.program Profile60C300 @16:55:45.98 Profile60C300 should complete in < 1:45:21 @16:55:46.61 PCR.run 2:16:41.25 @16:56:31.47 Stroke completed -- waiting for pressure to equalize ... @16:56:41.71 Intake.select :close @16:56:41.87 SSV.dial :exhaust @16:56:42.68 Exhaust.select :open @16:56:42.79 SS.reconfigure SSexhale @16:56:42.84 SS.seek 0ml @16:56:52.23 Exhaust.select :close @16:56:52.35 SSV.dial CBV @16:56:52.38 CBV.dial SSV @16:56:53.23 Allowing 130s for 25.0ml of remaining 375.0ml @16:56:53.24 Intake.select :open @16:56:55.44 Intake @35.3psia, vacuum from 20.0 to 24.0psi @16:56:55.46 SS.reconfigure SSinhale.with maxSpeed: 15 @16:56:55.51 SS.seek 25ml @16:58:52.06 Stroke completed -- waiting for pressure to equalize ... @16:59:02.94 Intake.select :close @16:59:03.10 SSV.dial :exhaust @16:59:03.92 Exhaust.select :open @16:59:04.03 SS.reconfigure SSexhale @16:59:04.09 SS.seek 0ml @16:59:14.01 Exhaust.select :close @16:59:14.13 SSV.dial CBV @16:59:14.16 CBV.dial SSV @16:59:14.98 Allowing 130s for 25.0ml of remaining 350.0ml @16:59:14.99 Intake.select :open @16:59:17.18 Intake @35.1psia, vacuum from 20.0 to 24.0psi @16:59:17.20 SS.reconfigure SSinhale.with maxSpeed: 15 @16:59:17.25 SS.seek 25ml @17:01:04.55 Vacuum too high -- waiting up to 40s @17:01:07.21 Intake @35.2psia, vacuum from 20.0 to 24.0psi @17:01:07.23 SS.reconfigure SSinhale.with maxSpeed: 14 @17:01:07.28 SS.seek 25ml @17:01:18.19 Stroke completed -- waiting for pressure to equalize ... @17:01:25.01 SS.reconfigure SSconfig @17:01:25.06 SS.hold @17:01:25.19 Intake.select :close @17:01:25.35 ShallowSampler::Clogged in WCR -- progress too slow after 1175.0ml @17:01:25.37 SSV.dial :exhaust @17:01:26.18 Exhaust.select :open @17:01:26.29 SS.reconfigure SSexhale @17:01:26.34 SS.seek 0ml @17:01:35.58 Exhaust.select :close @17:01:35.70 Sampled 1175.0ml @17:01:35.72 Drying puck before fixing for 4:00 @17:01:35.73 CBV.dial :waste2b @17:01:36.54 delay 1:00 @17:02:36.56 CSV.dial :air @17:02:37.05 CS.reconfigure CSfast @17:02:37.11 CS.seek 10ml @17:03:01.41 Pumping wcr puck from tube 3 up to 55psi... @17:03:01.42 CBV.dial SSV @17:03:02.79 CBV.dial between CSV and waste2b @17:03:03.82 CSV.dial between air and CTV @17:03:04.81 CS.seek 6.887ml @17:03:13.09 CTV.dial CSV,avoiding: :air @17:03:13.12 CSV.dial CTV @17:03:13.98 CS.reconfigure CSpressurize @17:03:14.04 CS.seek 0ml @17:03:32.22 CSV.dial :air @17:03:32.70 CS.reconfigure CSfast @17:03:32.76 CS.seek 10ml @17:03:56.90 CSV.dial between air and CTV @17:03:57.26 CS.seek 2.445ml @17:04:15.97 CTV.dial CSV,avoiding: :air @17:04:16.00 CSV.dial CTV @17:04:16.33 CS.reconfigure CSpressurize @17:04:16.39 CS.seek 0ml @17:04:20.55 CBV.dial :waste2b @17:04:20.91 CS.reconfigure CSconfig @17:04:20.96 CS.seek :empty @17:04:24.69 delay 1:00 @17:05:24.72 CTV.dial :air,avoiding: :intake @17:05:26.10 Fix #1 of 2 (with rnal) for 2:00 @17:05:26.12 CSV.dial CSR @17:05:26.64 Pulling 2ml of CSR.rnal @17:05:26.66 CSR.select :rnal @17:05:26.78 CS.seek 2ml @17:05:33.00 delay 5 seconds @17:05:38.02 CSR.select :air @17:05:38.15 CS.reconfigure CSfast @17:05:38.20 CS.seek 10ml @17:05:57.63 Pumping wcr puck from tube 3 up to 55psi... @17:05:57.64 CBV.dial SSV @17:05:59.02 CBV.dial between CSV and waste2b @17:06:00.06 CSV.dial between air and CTV @17:06:00.71 CS.seek 6.851ml @17:06:09.03 CTV.dial CSV,avoiding: :air @17:06:09.06 CSV.dial CTV @17:06:10.55 CS.reconfigure CSpressurize @17:06:10.61 CS.seek 0ml @17:06:28.18 delay 2:00 @17:08:28.21 CBV.dial :waste2b @17:08:28.57 CS.reconfigure CSconfig @17:08:28.62 CS.seek :empty @17:08:29.97 delay 1:00 @17:09:30.00 CTV.dial :air,avoiding: :intake @17:09:31.38 Fix #2 of 2 (with rnal) for 2:00 @17:09:31.40 CSV.dial CSR @17:09:31.93 Pulling 2ml of CSR.rnal @17:09:31.94 CSR.select :rnal @17:09:32.06 CS.seek 2ml @17:09:38.19 delay 5 seconds @17:09:43.21 CSR.select :air @17:09:43.34 CS.reconfigure CSfast @17:09:43.39 CS.seek 10ml @17:10:02.82 Pumping wcr puck from tube 3 up to 55psi... @17:10:02.83 CBV.dial SSV @17:10:04.20 CBV.dial between CSV and waste2b @17:10:05.23 CSV.dial between air and CTV @17:10:05.89 CS.seek 6.839ml @17:10:14.26 CTV.dial CSV,avoiding: :air @17:10:14.29 CSV.dial CTV @17:10:15.76 CS.reconfigure CSpressurize @17:10:15.82 CS.seek 0ml @17:10:33.09 delay 2:00 @17:12:33.11 CBV.dial :waste2b @17:12:33.48 CS.reconfigure CSconfig @17:12:33.53 CS.seek :empty @17:12:35.41 delay 1:00 @17:13:35.44 CTV.dial :air,avoiding: :intake @17:13:36.89 Storing wcr puck in Collection Clamp to tube 2 @17:13:37.09 SC.reconfigure SCtight @17:13:37.14 SC.seek 2 @17:13:39.31 SC.reconfigure SCloose @17:13:39.54 Elbow.reconfigure TightElbow @17:13:39.59 Elbow.seek 2 @17:13:41.47 Forearm.reconfigure TightForearm @17:13:41.52 Forearm.seek 2 + 700 counts @17:13:42.53 SE.reconfigure SEpick @17:13:42.60 SE.seek beamBreak @17:13:45.98 SE.configure SEconfig @17:13:46.16 Elbow.reconfigure LimpElbow @17:13:46.21 Forearm.reconfigure LooseForearm @17:13:46.27 Forearm.seek 2 @17:13:46.59 Elbow.hold @17:13:46.67 Elbow.reconfigure TightElbow @17:13:46.78 SE.seek 3.08 Pucks below Top Plate @17:13:47.27 Forearm.force -20 @17:13:47.32 Forearm.seek 2 + 336 counts @17:13:47.66 Forearm.reconfigure TightForearm @17:13:47.72 Forearm.seek 2 + 700 counts @17:13:47.97 SE.seek 4.08 Pucks below Top Plate @17:13:48.65 Forearm.reconfigure LooseForearm @17:13:48.71 Forearm.seek :retract @17:13:49.66 Elbow.seek CC @17:13:51.39 Elbow.reconfigure LooseElbow @17:13:51.50 Forearm.seek CC @17:13:52.49 Hand.close @17:13:53.10 CC.seek :open @17:13:57.62 Forearm.seek :retract @17:13:58.60 Elbow.reconfigure TightElbow @17:13:58.65 Elbow.seek 2 @17:14:00.50 Elbow.reconfigure LimpElbow @17:14:00.55 Forearm.seek 2 @17:14:01.57 Elbow.hold @17:14:01.65 Elbow.reconfigure TightElbow @17:14:01.70 Hand.open @17:14:02.48 Forearm.force -20 @17:14:03.12 Forearm.seek 2 + 349 counts @17:14:03.48 Forearm.reconfigure TightForearm @17:14:03.54 Forearm.seek 2 + 700 counts @17:14:04.02 SE.seek :down @17:14:05.17 Forearm.reconfigure LooseForearm @17:14:05.22 Forearm.seek :retract @17:14:06.42 SE.coast @17:14:06.46 SC.coast @17:14:06.51 Starting finalPurge @17:14:06.52 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:14:06.60 Hand.open @17:14:07.52 Elbow.seek FlushPuck::Garage @17:14:09.55 Elbow.reconfigure LooseElbow @17:14:09.67 Forearm.seek :garage @17:14:10.66 Hand.close @17:14:11.49 Forearm.seek :retract @17:14:12.49 Elbow.reconfigure TightElbow @17:14:12.54 Elbow.seek CC @17:14:15.51 Elbow.reconfigure LooseElbow @17:14:15.62 Forearm.reconfigure TightForearm @17:14:15.67 Forearm.seek CC @17:14:16.94 CC.seek :closed @17:14:21.20 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.04 @17:14:21.28 Hand.open @17:14:21.92 Forearm.reconfigure LooseForearm @17:14:21.97 Forearm.seek :retract @17:14:22.96 Flushing the Collection Valve::Manifold @17:14:23.10 CSV.dial CSR @17:14:23.63 Pulling 5ml of CSR.flush @17:14:23.64 CSR.select :flush @17:14:23.77 CS.seek 5ml @17:14:37.51 delay 5 seconds @17:14:42.53 CSR.select :air @17:14:42.71 CS.reconfigure CSfast @17:14:42.76 CS.seek 7.5ml @17:14:49.47 CSV.dial :waste @17:14:50.84 CS.reconfigure CSconfig @17:14:50.89 CS.seek 0ml @17:15:11.01 Flushing lower manifold's waste1 port @17:15:11.15 CSV.dial :flush @17:15:11.62 CS.reconfigure CSfast @17:15:11.67 CS.seek 5ml @17:15:24.57 delay 5 seconds @17:15:29.58 CSV.dial :air @17:15:30.25 CS.seek 7.001ml @17:15:35.92 CSV.dial CTV @17:15:35.95
CTV.dial CSV @17:15:37.40 CBV.dial :waste1 @17:15:38.21 CS.seek 0ml @17:15:55.42 Flushing lower manifold's waste2b port @17:15:55.56 CSV.dial :flush @17:15:56.35 CS.seek 5ml @17:16:08.92 delay 5 seconds @17:16:13.93 CSV.dial :air @17:16:14.50 CS.seek 7ml @17:16:20.07 CSV.dial CTV @17:16:20.73
CTV.dial CSV @17:16:21.21 CBV.dial :waste2b @17:16:22.04 CS.seek 0ml @17:16:39.17 Flushing upper manifold @17:16:39.31 CSV.dial :flush @17:16:40.10 CS.seek 5ml @17:16:52.67 delay 5 seconds @17:16:57.68 CSV.dial :air @17:16:58.25 CS.seek 7ml @17:17:03.89 CSV.dial CBV @17:17:03.92
CBV.dial CSV @17:17:05.06 CTV.dial :air @17:17:06.43 CS.seek 0ml @17:17:23.45 delay 7 seconds @17:17:30.47 CS.seek 3ml @17:17:38.45 CSV.dial :waste @17:17:38.94 CS.seek 0ml @17:17:46.99 CSV.dial :flush @17:17:47.46 CS.seek 3ml @17:17:55.48 delay 5 seconds @17:18:00.50 CSV.dial :air @17:18:01.05 CS.seek 5ml @17:18:06.67 CSV.dial :air @17:18:06.69 CS.seek 0ml @17:18:19.20 delay 7 seconds @17:18:26.23 CS.seek 3ml @17:18:34.11 CSV.dial :waste @17:18:34.90 CS.seek 0ml @17:18:42.89 Disinfecting external Intake with mfbkill @17:18:43.03 CSV.dial CSR @17:18:44.45 Pulling 8ml of CSR.mfbkill @17:18:44.46 CSR.select :mfbkill @17:18:44.59 CS.seek 8ml @17:19:03.89 delay 5 seconds @17:19:08.90 CSR.select :air @17:19:09.04 CS.seek 10ml @17:19:14.67 CSV.dial CBV @17:19:14.70
CBV.dial CSV @17:19:15.78 CTV.dial :intake @17:19:16.58 Intake.select :open @17:19:16.72 CS.seek 2ml @17:19:37.00 Intake.select :close @17:19:37.12 CTV.dial :air @17:19:37.90 CS.reconfigure CSconfig @17:19:37.95 CS.seek 0ml @17:19:44.11 Flushing lower manifold's waste1 port @17:19:44.12 CSV.dial CSR @17:19:45.26 Pulling 4ml of CSR.mfbkill @17:19:45.27 CSR.select :mfbkill @17:19:45.40 CS.seek 4ml @17:19:56.61 delay 5 seconds @17:20:01.63 CSR.select :air @17:20:01.79 CS.reconfigure CSfast @17:20:01.84 CS.seek 10ml @17:20:16.58 CSV.dial CTV @17:20:16.61
CTV.dial CSV @17:20:18.06 CBV.dial :waste1 @17:20:19.18 CS.seek 0ml @17:20:43.14 Flushing lower manifold's waste2b port @17:20:43.15 CSV.dial CSR @17:20:43.71 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @17:20:43.84 CS.reconfigure CSconfig @17:20:43.88 CS.seek 4ml @17:20:55.12 delay 5 seconds @17:21:00.14 CSR.select :air @17:21:00.27 CS.reconfigure CSfast @17:21:00.32 CS.seek 10ml @17:21:15.08 CSV.dial CTV @17:21:15.11
CTV.dial CSV @17:21:15.59 CBV.dial :waste2b @17:21:16.42 CS.seek 0ml @17:21:40.45 Killing upper manifold @17:21:40.46 CSV.dial CSR @17:21:41.02 Pulling 4ml of CSR.mfbkill @17:21:41.03 CSR.select :mfbkill @17:21:41.16 CS.reconfigure CSconfig @17:21:41.20 CS.seek 4ml @17:21:52.48 delay 5 seconds @17:21:57.50 CSR.select :air @17:21:57.63 CS.reconfigure CSfast @17:21:57.68 CS.seek 10ml @17:22:12.36 CSV.dial CBV @17:22:12.39
CBV.dial CSV @17:22:13.50 CTV.dial :air @17:22:14.88 CS.seek 0ml @17:22:38.64 delay 7 seconds @17:22:45.66 CS.seek 3ml @17:22:53.80 CSV.dial :waste @17:22:54.28 CS.seek 0ml @17:23:02.17 Flushing upper manifold @17:23:02.31 CSV.dial :flush @17:23:02.78 CS.seek 5ml @17:23:15.24 delay 5 seconds @17:23:20.25 CSV.dial :air @17:23:20.83 CS.seek 7ml @17:23:26.42 CSV.dial CBV @17:23:26.45
CBV.dial CSV @17:23:27.55 CTV.dial :air @17:23:27.57 CS.seek 0ml @17:23:44.58 delay 7 seconds @17:23:51.60 CS.seek 3ml @17:23:59.54 CSV.dial :waste @17:24:00.04 CS.seek 0ml @17:24:08.08 CSV.dial :flush @17:24:08.56 CS.seek 3ml @17:24:16.52 delay 5 seconds @17:24:21.53 CSV.dial :air @17:24:22.09 CS.seek 5ml @17:24:27.71 CSV.dial :air @17:24:27.73 CS.seek 0ml @17:24:40.17 delay 7 seconds @17:24:47.19 CS.seek 3ml @17:24:55.16 CSV.dial :waste @17:24:56.57 CS.seek 0ml @17:25:04.49 Flushing lower manifold's waste1 port @17:25:04.64 CSV.dial :flush @17:25:05.12 CS.seek 5ml @17:25:17.58 delay 5 seconds @17:25:22.59 CSV.dial :air @17:25:23.15 CS.seek 7ml @17:25:28.74 CSV.dial CTV @17:25:28.77
CTV.dial CSV @17:25:30.22 CBV.dial :waste1 @17:25:30.72 CS.seek 0ml @17:25:47.58 Flushing lower manifold's waste2b port @17:25:47.72 CSV.dial :flush @17:25:48.51 CS.seek 5ml @17:26:00.96 delay 5 seconds @17:26:05.97 CSV.dial :air @17:26:06.54 CS.seek 7ml @17:26:12.11 CSV.dial CTV @17:26:12.14
CTV.dial CSV @17:26:12.61 CBV.dial :waste2b @17:26:13.44 CS.seek 0ml @17:26:30.39 Disinfecting external Intake with kill @17:26:30.53 CSV.dial CSR @17:26:31.06 Pulling 8ml of CSR.kill @17:26:31.07 CSR.select :kill @17:26:31.20 CS.seek 8ml @17:26:50.46 delay 5 seconds @17:26:55.48 CSR.select :air @17:26:55.62 CS.seek 10ml @17:27:01.36 CSV.dial CBV @17:27:01.39
CBV.dial CSV @17:27:02.52 CTV.dial :intake @17:27:03.30 Intake.select :open @17:27:03.42 CS.seek 2ml @17:27:23.45 Intake.select :close @17:27:23.57 CTV.dial :air @17:27:24.35 CS.reconfigure CSconfig @17:27:24.40 CS.seek 0ml @17:27:30.55 CSV.dial CSR @17:27:31.67 Pulling 5ml of CSR.flush @17:27:31.68 CSR.select :flush @17:27:31.83 CS.seek 5ml @17:27:45.83 delay 5 seconds @17:27:50.85 CSR.select :air @17:27:50.99 CS.seek 10ml @17:28:04.70 CSV.dial CBV @17:28:05.79 CS.seek 0ml @17:28:32.37 Garaging FlushPuck in Collection Clamp @17:28:32.45 Elbow.reconfigure TightElbow @17:28:32.50 Elbow.seek CC @17:28:32.77 Elbow.reconfigure LooseElbow @17:28:32.87 Forearm.seek CC @17:28:33.83 Hand.close @17:28:34.49 CC.seek :open @17:28:39.49 Forearm.seek :retract @17:28:40.49 Elbow.reconfigure TightElbow @17:28:40.54 Elbow.seek :garage @17:28:43.63 Elbow.reconfigure LooseElbow @17:28:43.75 Forearm.reconfigure TightForearm @17:28:43.80 Forearm.seek :garage @17:28:44.97 Hand.open @17:28:45.69 Forearm.reconfigure LooseForearm @17:28:45.74 Forearm.seek :retract @17:28:46.78 Starting initialPurge @17:28:46.79 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:28:46.87 Hand.open @17:28:47.19 Elbow.reconfigure TightElbow @17:28:47.24 Elbow.seek FlushPuck::Garage @17:28:47.36 Elbow.reconfigure LooseElbow @17:28:47.47 Forearm.seek :garage @17:28:48.51 Hand.close @17:28:49.28 Forearm.seek :retract @17:28:50.31 Elbow.reconfigure TightElbow @17:28:50.36 Elbow.seek CC @17:28:53.30 Elbow.reconfigure LooseElbow @17:28:53.40 Forearm.reconfigure TightForearm @17:28:53.45 Forearm.seek CC @17:28:54.86 CC.seek :closed @17:28:59.09 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.08 @17:28:59.18 Hand.open @17:28:59.95 Forearm.reconfigure LooseForearm @17:29:00.00 Forearm.seek :retract @17:29:00.95 Flushing the Collection Valve::Manifold @17:29:01.09 CSV.dial CSR @17:29:02.22 Pulling 5ml of CSR.flush @17:29:02.23 CSR.select :flush @17:29:02.36 CS.seek 5ml @17:29:16.21 delay 5 seconds @17:29:21.23 CSR.select :air @17:29:21.41 CS.reconfigure CSfast @17:29:21.46 CS.seek 7.501ml @17:29:28.24 CSV.dial :waste @17:29:29.60 CS.reconfigure CSconfig @17:29:29.65 CS.seek 0ml @17:29:49.74 Purging external Intake @17:29:49.87 CSV.dial :flush @17:29:50.36 CS.reconfigure CSfast @17:29:50.41 CS.seek 10ml @17:30:14.05 delay 5 seconds @17:30:19.07 CSV.dial CBV @17:30:19.10
CBV.dial CSV @17:30:19.90 CTV.dial :intake @17:30:20.69 Intake.select :open @17:30:20.81 CS.seek 0ml @17:30:45.67 Intake.select :close @17:30:45.79 CTV.dial :air @17:30:47.21 Flushing upper manifold @17:30:47.35 CSV.dial :flush @17:30:48.14 CS.seek 5ml @17:31:00.65 delay 5 seconds @17:31:05.66 CSV.dial :air @17:31:06.22 CS.seek 7ml @17:31:11.93 CSV.dial CBV @17:31:11.96
CBV.dial CSV @17:31:13.04 CTV.dial :air @17:31:13.06 CS.seek 0ml @17:31:30.15 delay 7 seconds @17:31:37.17 CS.seek 3ml @17:31:45.23 CSV.dial :waste @17:31:45.72 CS.seek 0ml @17:31:53.89 CSV.dial :flush @17:31:54.36 CS.seek 3ml @17:32:02.27 delay 5 seconds @17:32:07.28 CSV.dial :air @17:32:07.95 CS.seek 5ml @17:32:13.61 CSV.dial :air @17:32:13.64 CS.seek 0ml @17:32:26.15 delay 7 seconds @17:32:33.17 CS.seek 3ml @17:32:41.02 CSV.dial :waste @17:32:41.79 CS.seek 0ml @17:32:49.62 Garaging FlushPuck in Collection Clamp @17:32:49.70 Elbow.reconfigure TightElbow @17:32:49.75 Elbow.seek CC @17:32:50.01 Elbow.reconfigure LooseElbow @17:32:50.12 Forearm.seek CC @17:32:51.07 Hand.close @17:32:51.75 CC.seek :open @17:32:56.12 Forearm.seek :retract @17:32:57.13 Elbow.reconfigure TightElbow @17:32:57.18 Elbow.seek :garage @17:33:00.22 Elbow.reconfigure LooseElbow @17:33:00.33 Forearm.reconfigure TightForearm @17:33:00.38 Forearm.seek :garage @17:33:01.56 Hand.open @17:33:02.32 Forearm.reconfigure LooseForearm @17:33:02.37 Forearm.seek :retract @17:33:03.35 Completed [phasewithdil] begun 14:00:26 @17:33:03.37 Preparing to sleep until 14PM @17:33:03.41 deferred until MFBPCR finish @17:33:03.43
Waiting for MFBPCR til 14PM @17:33:03.46 Continuing at 14:00CEST09-Oct-18 @17:33:03.53 GoodNews.email "Then, in tube 3: phasewithdil\n/home/esp/esp2/mission/18tundeployv2dil.rb:118\n{1 => 22, 2 => 5, 3 => 17}\nCan@17:33:03, 14.7C, 69% humidity, 14.3psia, 13.294V, 0.758A, 0.752A avg, 10.08W",Subject: "Waiting until 14:00CEST09-Oct-18" @18:18:10.32 PCR.off @18:18:11.90 PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0816h1150ml.pcr @18:18:11.98 Cleaning PCR @18:18:11.99 AFV.select :carrierSolution @18:18:12.10 delay 2 seconds @18:18:14.13 AS.reconfigure ASconfig @18:18:14.18 AS.seek 0.5ml @18:18:24.48 delay 1 second @18:18:25.49 AFV.select :holdingCoil @18:18:25.60 ASV.dial :waste1 @18:18:25.96 ATV.dial :air3 @18:18:27.17 AS.reconfigure ASslow1 @18:18:27.22 AS.seek 0.51ml @18:18:29.49 delay 2 seconds @18:18:31.50 ATV.dial :bleach @18:18:32.11 AS.seek 0.81ml @18:19:32.71 delay 3 seconds @18:19:35.73 ATV.dial :air3 @18:19:36.26 delay 3 seconds @18:19:39.35 AS.seek 0.835ml @18:19:44.68 delay 3 seconds @18:19:47.70 ATV.dial :bleach @18:19:48.30 AS.seek 0.82ml @18:19:51.60 delay 3 seconds @18:19:54.63 ARV.dial :waste @18:19:55.20 delay 3 seconds @18:19:58.22 ATV.dial :ARV @18:19:59.06 AS.reconfigure ASconfig @18:19:59.11 AS.seek 0.47ml @18:20:19.48 AFV.select :carrierSolution @18:20:19.60 delay 2 seconds @18:20:21.63 AS.seek 1ml @18:20:52.38 delay 1 second @18:20:53.39 AFV.select :holdingCoil @18:20:53.50 delay 1 second @18:20:54.53 AS.reconfigure ASfast @18:20:54.58 AS.seek 0ml @18:21:21.91 AFV.select :carrierSolution @18:21:22.03 delay 2 seconds @18:21:24.06 AS.reconfigure ASconfig @18:21:24.11 AS.seek 1ml @18:22:21.84 delay 1 second @18:22:22.86 AFV.select :holdingCoil @18:22:22.97 delay 1 second @18:22:24.00 AS.reconfigure ASfast @18:22:24.05 AS.seek 0ml @18:22:51.38 AFV.select :carrierSolution @18:22:51.50 delay 2 seconds @18:22:53.53 AS.reconfigure ASconfig @18:22:53.58 AS.seek 1ml @18:23:51.37 delay 1 second @18:23:52.38 AFV.select :holdingCoil @18:23:52.49 delay 1 second @18:23:53.52 AS.reconfigure ASfast @18:23:53.57 AS.seek 0ml @18:24:20.92 ARV.dial between carrier and air1 @18:24:21.40 ATV.dial :air3 @18:24:22.61 AS.reconfigure ASslow1 @18:24:22.66 AS.seek 0.01ml @18:24:25.01 delay 2 seconds @18:24:27.02 ATV.dial :bleach @18:24:27.63 AS.seek 0.81ml @18:27:08.78 delay 3 seconds @18:27:11.80 ATV.dial :air3 @18:27:12.32 delay 3 seconds @18:27:15.41 AS.seek 0.835ml @18:27:20.69 delay 3 seconds @18:27:23.71 ATV.dial :bleach @18:27:24.92 AS.seek 0.82ml @18:27:28.21 delay 3 seconds @18:27:31.23 ATV.dial PCR @18:27:31.62 AS.reconfigure ASconfig @18:27:31.67 AS.seek :empty @18:28:19.14 PCR.program :bleachProfile @18:28:19.20 PCR program should complete in < 2:45 @18:28:19.21 PCR.run 10:00 @18:31:30.12 PCR.off @18:31:30.15 AFV.select :carrierSolution @18:31:30.27 delay 2 seconds @18:31:32.30 AS.seek 1ml @18:32:30.12 delay 1 second @18:32:31.14 AFV.select :holdingCoil @18:32:31.25 delay 1 second @18:32:32.28 AS.reconfigure ASfast @18:32:32.33 AS.seek 0ml @18:32:59.67 delay 3 seconds @18:33:02.69 AFV.select :carrierSolution @18:33:02.80 delay 2 seconds @18:33:04.83 AS.reconfigure ASconfig @18:33:04.89 AS.seek 1ml @18:34:02.67 delay 1 second @18:34:03.68 AFV.select :holdingCoil @18:34:03.79 delay 1 second @18:34:04.82 AS.reconfigure ASfast @18:34:04.87 AS.seek 0ml @18:34:32.23 delay 3 seconds @18:34:35.25 AFV.select :carrierSolution @18:34:35.36 delay 2 seconds @18:34:37.39 AS.reconfigure ASconfig @18:34:37.45 AS.seek 1ml @18:35:35.25 delay 1 second @18:35:36.26 AFV.select :holdingCoil @18:35:36.37 delay 1 second @18:35:37.40 AS.reconfigure ASfast @18:35:37.45 AS.seek 0ml @18:36:04.80 delay 3 seconds @18:36:07.82 AFV.select :carrierSolution @18:36:07.93 delay 2 seconds @18:36:09.97 AS.reconfigure ASconfig @18:36:10.01 AS.seek 1ml @18:37:07.81 delay 1 second @18:37:08.82 AFV.select :holdingCoil @18:37:08.93 delay 1 second @18:37:09.96 AS.reconfigure ASfast @18:37:10.01 AS.seek 0ml @18:37:37.36 delay 3 seconds @18:37:40.38 AFV.select :carrierSolution @18:37:40.49 delay 2 seconds @18:37:42.52 AS.reconfigure ASconfig @18:37:42.58 AS.seek 1ml @18:38:40.39 delay 1 second @18:38:41.40 AFV.select :holdingCoil @18:38:41.51 delay 1 second @18:38:42.54 AS.reconfigure ASnorm @18:38:42.59 AS.seek 0ml @18:39:33.32 Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0818h1150ml.pcr @18:39:33.34 Building PCR Slug from ASV with MM2 mix, PP3 primer @18:39:33.35 Priming with carrier @18:39:33.36 ATV.dial ARV @18:39:34.05 ARV.dial :carrier @18:39:34.40 AS.seek 0.5ml @18:40:00.02 AFV.select :carrierSolution @18:40:00.14 delay 2 seconds @18:40:02.17 AS.reconfigure ASconfig @18:40:02.22 AS.seek 1ml @18:40:31.23 delay 1 second @18:40:32.25 AFV.select :holdingCoil @18:40:32.36 delay 1 second @18:40:33.38 ATV.dial :waste @18:40:34.91 AS.reconfigure ASnorm @18:40:34.96 AS.seek :empty @18:41:25.69 AFV.select :carrierSolution @18:41:25.81 delay 2 seconds @18:41:27.84 AS.reconfigure ASconfig @18:41:27.89 AS.seek 0.5ml @18:41:56.99 delay 1 second @18:41:58.00 AFV.select :holdingCoil @18:41:58.11 Building Slug @18:41:58.12 ATV.dial :ARV @18:41:59.72 delay 2 seconds @18:42:01.74 ARV.dial :air1 @18:42:02.12 delay 2 seconds @18:42:04.21 AS.reconfigure ASslow2 @18:42:04.26 AS.seek 0.506ml @18:42:10.45 delay 2 seconds @18:42:12.47 ARV.dial :MM2 @18:42:13.84 delay 2 seconds @18:42:15.87 Adding MM2 @18:42:15.95 AS.seek 0.524ml @18:42:34.20 delay 25 seconds @18:42:59.22 ARV.dial :PP3 @18:42:59.60 Adding PP3 @18:42:59.62 delay 2 seconds @18:43:01.70 AS.seek 0.53ml @18:43:08.06 delay 10 seconds @18:43:18.08 ARV.dial :air2 @18:43:19.08 adding ARV air @18:43:19.09 delay 2 seconds @18:43:21.18 AS.seek 0.536ml @18:43:27.55 delay 5 seconds @18:43:32.57 ARV.dial :carrier @18:43:33.77 delay 7 seconds @18:43:40.86 AS.seek 0.594ml @18:44:39.41 delay 5 seconds @18:44:44.43 ATV.dial :ASV @18:44:44.83 delay 2 seconds @18:44:46.92 AS.seek 0.6ml @18:44:53.36 delay 4 seconds @18:44:57.38 ATV.dial ARV @18:44:57.91 delay 2 seconds @18:44:59.93 ARV.dial :carrier @18:44:59.95 delay 2 seconds @18:45:02.04 AS.seek 0.65ml @18:45:52.64 delay 4 seconds @18:45:56.66 ATV.dial PCR @18:45:57.24 ASV.dial :waste1 @18:45:57.25 ARV.dial :air1 @18:45:57.65 Positioning Slug by 0.324ml @18:45:57.74 AS.seek 0.326ml @18:51:23.83 delay 5 seconds @18:51:28.85 ATV.dial ARV @18:51:29.58 ASV.dial :PCRlock @18:51:29.96 delay 5 seconds @18:51:34.99 Running Rp Profile60C300 thermocycling @18:51:35.00 PCR.program Profile60C300 @18:51:35.14 Profile60C300 should complete in < 1:45:21 @18:51:35.15 PCR.run 2:16:41.25 @20:13:29.97 PCR.off @20:13:31.55 PCR for Rp completed -- closed /var/log/esp/Rp18oct0818h1150ml.pcr @20:13:31.62 Cleaning PCR @20:13:31.63 AFV.select :carrierSolution @20:13:31.74 delay 2 seconds @20:13:33.77 AS.reconfigure ASconfig @20:13:33.82 AS.seek 0.5ml @20:13:44.16 delay 1 second @20:13:45.17 AFV.select :holdingCoil @20:13:45.28 ASV.dial :waste1 @20:13:45.66 ATV.dial :air3 @20:13:46.87 AS.reconfigure ASslow1 @20:13:46.92 AS.seek 0.51ml @20:13:49.21 delay 2 seconds @20:13:51.22 ATV.dial :bleach @20:13:51.82 AS.seek 0.81ml @20:14:52.41 delay 3 seconds @20:14:55.43 ATV.dial :air3 @20:14:55.95 delay 3 seconds @20:14:59.04 AS.seek 0.835ml @20:15:04.39 delay 3 seconds @20:15:07.41 ATV.dial :bleach @20:15:08.01 AS.seek 0.82ml @20:15:11.28 delay 3 seconds @20:15:14.30 ARV.dial :waste @20:15:14.89 delay 3 seconds @20:15:17.91 ATV.dial :ARV @20:15:18.75 AS.reconfigure ASconfig @20:15:18.81 AS.seek 0.47ml @20:15:39.21 AFV.select :carrierSolution @20:15:39.33 delay 2 seconds @20:15:41.36 AS.seek 1ml @20:16:12.11 delay 1 second @20:16:13.12 AFV.select :holdingCoil @20:16:13.23 delay 1 second @20:16:14.26 AS.reconfigure ASfast @20:16:14.31 AS.seek 0ml @20:16:41.65 AFV.select :carrierSolution @20:16:41.77 delay 2 seconds @20:16:43.80 AS.reconfigure ASconfig @20:16:43.85 AS.seek 1ml @20:17:41.63 delay 1 second @20:17:42.64 AFV.select :holdingCoil @20:17:42.76 delay 1 second @20:17:43.79 AS.reconfigure ASfast @20:17:43.84 AS.seek 0ml @20:18:11.16 AFV.select :carrierSolution @20:18:11.28 delay 2 seconds @20:18:13.31 AS.reconfigure ASconfig @20:18:13.36 AS.seek 1ml @20:19:11.11 delay 1 second @20:19:12.12 AFV.select :holdingCoil @20:19:12.23 delay 1 second @20:19:13.26 AS.reconfigure ASfast @20:19:13.31 AS.seek 0ml @20:19:40.65 ARV.dial between carrier and air1 @20:19:41.14 ATV.dial :air3 @20:19:42.35 AS.reconfigure ASslow1 @20:19:42.40 AS.seek 0.01ml @20:19:44.66 delay 2 seconds @20:19:46.67 ATV.dial :bleach @20:19:47.88 AS.seek 0.81ml @20:22:29.02 delay 3 seconds @20:22:32.04 ATV.dial :air3 @20:22:32.57 delay 3 seconds @20:22:35.66 AS.seek 0.835ml @20:22:40.97 delay 3 seconds @20:22:43.99 ATV.dial :bleach @20:22:44.59 AS.seek 0.82ml @20:22:47.89 delay 3 seconds @20:22:50.91 ATV.dial PCR @20:22:51.30 AS.reconfigure ASconfig @20:22:51.36 AS.seek :empty @20:23:38.82 PCR.program :bleachProfile @20:23:38.88 PCR program should complete in < 2:45 @20:23:38.89 PCR.run 10:00 @20:26:49.80 PCR.off @20:26:49.82 AFV.select :carrierSolution @20:26:49.94 delay 2 seconds @20:26:51.97 AS.seek 1ml @20:27:49.80 delay 1 second @20:27:50.81 AFV.select :holdingCoil @20:27:50.92 delay 1 second @20:27:51.95 AS.reconfigure ASfast @20:27:52.00 AS.seek 0ml @20:28:19.33 delay 3 seconds @20:28:22.35 AFV.select :carrierSolution @20:28:22.46 delay 2 seconds @20:28:24.49 AS.reconfigure ASconfig @20:28:24.55 AS.seek 1ml @20:29:22.35 delay 1 second @20:29:23.36 AFV.select :holdingCoil @20:29:23.47 delay 1 second @20:29:24.50 AS.reconfigure ASfast @20:29:24.55 AS.seek 0ml @20:29:51.91 delay 3 seconds @20:29:54.93 AFV.select :carrierSolution @20:29:55.04 delay 2 seconds @20:29:57.07 AS.reconfigure ASconfig @20:29:57.12 AS.seek 1ml @20:30:54.94 delay 1 second @20:30:55.96 AFV.select :holdingCoil @20:30:56.07 delay 1 second @20:30:57.10 AS.reconfigure ASfast @20:30:57.15 AS.seek 0ml @20:31:24.52 delay 3 seconds @20:31:27.54 AFV.select :carrierSolution @20:31:27.65 delay 2 seconds @20:31:29.68 AS.reconfigure ASconfig @20:31:29.74 AS.seek 1ml @20:32:27.55 delay 1 second @20:32:28.56 AFV.select :holdingCoil @20:32:28.67 delay 1 second @20:32:29.70 AS.reconfigure ASfast @20:32:29.75 AS.seek 0ml @20:32:57.10 delay 3 seconds @20:33:00.12 AFV.select :carrierSolution @20:33:00.23 delay 2 seconds @20:33:02.26 AS.reconfigure ASconfig @20:33:02.31 AS.seek 1ml @20:34:00.10 delay 1 second @20:34:01.11 AFV.select :holdingCoil @20:34:01.22 delay 1 second @20:34:02.25 AS.reconfigure ASnorm @20:34:02.30 AS.seek 0ml @20:34:53.03 Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0820h1150ml.pcr @20:34:53.05 Building PCR Slug from ASV with MM3 mix, PP5 primer @20:34:53.06 Priming with carrier @20:34:53.07 ATV.dial ARV @20:34:53.76 ARV.dial :carrier @20:34:54.11 AS.seek 0.5ml @20:35:19.73 AFV.select :carrierSolution @20:35:19.85 delay 2 seconds @20:35:21.88 AS.reconfigure ASconfig @20:35:21.93 AS.seek 1ml @20:35:50.94 delay 1 second @20:35:51.95 AFV.select :holdingCoil @20:35:52.06 delay 1 second @20:35:53.08 ATV.dial :waste @20:35:54.62 AS.reconfigure ASnorm @20:35:54.67 AS.seek :empty @20:36:45.39 AFV.select :carrierSolution @20:36:45.51 delay 2 seconds @20:36:47.54 AS.reconfigure ASconfig @20:36:47.59 AS.seek 0.5ml @20:37:16.70 delay 1 second @20:37:17.71 AFV.select :holdingCoil @20:37:17.82 Building Slug @20:37:17.83 ATV.dial :ARV @20:37:19.45 delay 2 seconds @20:37:21.47 ARV.dial :air1 @20:37:21.97 delay 2 seconds @20:37:24.06 AS.reconfigure ASslow2 @20:37:24.11 AS.seek 0.506ml @20:37:30.45 delay 2 seconds @20:37:32.47 ARV.dial :MM3 @20:37:33.46 delay 2 seconds @20:37:35.49 Adding MM3 @20:37:36.20 AS.seek 0.524ml @20:37:54.49 delay 25 seconds @20:38:19.51 ARV.dial :PP5 @20:38:19.88 Adding PP5 @20:38:19.90 delay 2 seconds @20:38:21.99 AS.seek 0.53ml @20:38:28.26 delay 10 seconds @20:38:38.28 ARV.dial :air3 @20:38:38.66 adding ARV air @20:38:38.68 delay 2 seconds @20:38:40.76 AS.seek 0.536ml @20:38:47.01 delay 5 seconds @20:38:52.03 ARV.dial :carrier @20:38:53.23 delay 7 seconds @20:39:00.32 AS.seek 0.594ml @20:39:58.85 delay 5 seconds @20:40:03.87 ATV.dial :ASV @20:40:04.27 delay 2 seconds @20:40:06.36 AS.seek 0.6ml @20:40:12.59 delay 4 seconds @20:40:16.61 ATV.dial ARV @20:40:17.24 delay 2 seconds @20:40:19.26 ARV.dial :carrier @20:40:19.28 delay 2 seconds @20:40:21.37 AS.seek 0.65ml @20:41:11.84 delay 4 seconds @20:41:15.86 ATV.dial PCR @20:41:16.46 ASV.dial :waste1 @20:41:16.47 ARV.dial :air1 @20:41:16.86 Positioning Slug by 0.324ml @20:41:16.95 AS.seek 0.326ml @20:46:43.01 delay 5 seconds @20:46:48.03 ATV.dial ARV @20:46:48.87 ASV.dial :PCRlock @20:46:49.25 delay 5 seconds @20:46:54.28 Running Bb Profile60C300 thermocycling @20:46:54.29 PCR.program Profile60C300 @20:46:54.43 Profile60C300 should complete in < 1:45:21 @20:46:54.44 PCR.run 2:16:41.25 @22:08:42.63 PCR.off @22:08:44.21 PCR for Bb completed -- closed /var/log/esp/Bb18oct0820h1150ml.pcr @22:08:44.28 Cleaning PCR @22:08:44.29 AFV.select :carrierSolution @22:08:44.41 delay 2 seconds @22:08:46.44 AS.reconfigure ASconfig @22:08:46.49 AS.seek 0.5ml @22:08:56.79 delay 1 second @22:08:57.80 AFV.select :holdingCoil @22:08:57.91 ASV.dial :waste1 @22:08:58.27 ATV.dial :air3 @22:08:59.48 AS.reconfigure ASslow1 @22:08:59.53 AS.seek 0.51ml @22:09:01.82 delay 2 seconds @22:09:03.84 ATV.dial :bleach @22:09:04.45 AS.seek 0.81ml @22:10:05.10 delay 3 seconds @22:10:08.12 ATV.dial :air3 @22:10:08.65 delay 3 seconds @22:10:11.74 AS.seek 0.835ml @22:10:17.02 delay 3 seconds @22:10:20.04 ATV.dial :bleach @22:10:20.64 AS.seek 0.82ml @22:10:23.93 delay 3 seconds @22:10:26.95 ARV.dial :waste @22:10:27.52 delay 3 seconds @22:10:30.54 ATV.dial :ARV @22:10:31.39 AS.reconfigure ASconfig @22:10:31.44 AS.seek 0.47ml @22:10:51.81 AFV.select :carrierSolution @22:10:51.93 delay 2 seconds @22:10:53.96 AS.seek 1ml @22:11:24.76 delay 1 second @22:11:25.77 AFV.select :holdingCoil @22:11:25.88 delay 1 second @22:11:26.91 AS.reconfigure ASfast @22:11:26.96 AS.seek 0ml @22:11:54.31 AFV.select :carrierSolution @22:11:54.43 delay 2 seconds @22:11:56.46 AS.reconfigure ASconfig @22:11:56.51 AS.seek 1ml @22:12:54.30 delay 1 second @22:12:55.31 AFV.select :holdingCoil @22:12:55.42 delay 1 second @22:12:56.45 AS.reconfigure ASfast @22:12:56.51 AS.seek 0ml @22:13:23.86 AFV.select :carrierSolution @22:13:23.98 delay 2 seconds @22:13:26.01 AS.reconfigure ASconfig @22:13:26.06 AS.seek 1ml @22:14:23.85 delay 1 second @22:14:24.86 AFV.select :holdingCoil @22:14:24.97 delay 1 second @22:14:26.00 AS.reconfigure ASfast @22:14:26.05 AS.seek 0ml @22:14:53.37 ARV.dial between carrier and air1 @22:14:54.48 ATV.dial :air3 @22:14:55.69 AS.reconfigure ASslow1 @22:14:55.74 AS.seek 0.01ml @22:14:58.04 delay 2 seconds @22:15:00.06 ATV.dial :bleach @22:15:00.66 AS.seek 0.81ml @22:17:41.81 delay 3 seconds @22:17:44.83 ATV.dial :air3 @22:17:45.37 delay 3 seconds @22:17:48.46 AS.seek 0.835ml @22:17:53.75 delay 3 seconds @22:17:56.77 ATV.dial :bleach @22:17:57.38 AS.seek 0.82ml @22:18:00.67 delay 3 seconds @22:18:03.69 ATV.dial PCR @22:18:04.09 AS.reconfigure ASconfig @22:18:04.14 AS.seek :empty @22:18:51.61 PCR.program :bleachProfile @22:18:51.68 PCR program should complete in < 2:45 @22:18:51.69 PCR.run 10:00 @22:21:59.57 PCR.off @22:21:59.60 AFV.select :carrierSolution @22:21:59.71 delay 2 seconds @22:22:01.74 AS.seek 1ml @22:22:59.57 delay 1 second @22:23:00.58 AFV.select :holdingCoil @22:23:00.69 delay 1 second @22:23:01.72 AS.reconfigure ASfast @22:23:01.77 AS.seek 0ml @22:23:29.10 delay 3 seconds @22:23:32.12 AFV.select :carrierSolution @22:23:32.23 delay 2 seconds @22:23:34.26 AS.reconfigure ASconfig @22:23:34.32 AS.seek 1ml @22:24:32.07 delay 1 second @22:24:33.09 AFV.select :holdingCoil @22:24:33.20 delay 1 second @22:24:34.23 AS.reconfigure ASfast @22:24:34.28 AS.seek 0ml @22:25:01.62 delay 3 seconds @22:25:04.64 AFV.select :carrierSolution @22:25:04.75 delay 2 seconds @22:25:06.78 AS.reconfigure ASconfig @22:25:06.85 AS.seek 1ml @22:26:04.68 delay 1 second @22:26:05.69 AFV.select :holdingCoil @22:26:05.80 delay 1 second @22:26:06.83 AS.reconfigure ASfast @22:26:06.88 AS.seek 0ml @22:26:34.23 delay 3 seconds @22:26:37.25 AFV.select :carrierSolution @22:26:37.36 delay 2 seconds @22:26:39.39 AS.reconfigure ASconfig @22:26:39.45 AS.seek 1ml @22:27:37.23 delay 1 second @22:27:38.24 AFV.select :holdingCoil @22:27:38.35 delay 1 second @22:27:39.38 AS.reconfigure ASfast @22:27:39.43 AS.seek 0ml @22:28:06.78 delay 3 seconds @22:28:09.80 AFV.select :carrierSolution @22:28:09.91 delay 2 seconds @22:28:11.95 AS.reconfigure ASconfig @22:28:11.99 AS.seek 1ml @22:29:09.79 delay 1 second @22:29:10.80 AFV.select :holdingCoil @22:29:10.91 delay 1 second @22:29:11.94 AS.reconfigure ASnorm @22:29:11.99 AS.seek 0ml @22:30:02.71 Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0822h1150ml.pcr @22:30:02.73 Building PCR Slug from ASV with MM1 mix, PP2 primer @22:30:02.74 Priming with carrier @22:30:02.75 ATV.dial ARV @22:30:03.44 ARV.dial :carrier @22:30:03.79 AS.seek 0.5ml @22:30:29.36 AFV.select :carrierSolution @22:30:29.48 delay 2 seconds @22:30:31.52 AS.reconfigure ASconfig @22:30:31.57 AS.seek 1ml @22:31:00.61 delay 1 second @22:31:01.63 AFV.select :holdingCoil @22:31:01.74 delay 1 second @22:31:02.76 ATV.dial :waste @22:31:04.29 AS.reconfigure ASnorm @22:31:04.34 AS.seek :empty @22:31:55.07 AFV.select :carrierSolution @22:31:55.19 delay 2 seconds @22:31:57.22 AS.reconfigure ASconfig @22:31:57.27 AS.seek 0.5ml @22:32:26.37 delay 1 second @22:32:27.38 AFV.select :holdingCoil @22:32:27.49 Building Slug @22:32:27.50 ATV.dial :ARV @22:32:29.11 delay 2 seconds @22:32:31.13 ARV.dial :air1 @22:32:32.14 delay 2 seconds @22:32:34.22 AS.reconfigure ASslow2 @22:32:34.28 AS.seek 0.506ml @22:32:40.51 delay 2 seconds @22:32:42.53 ARV.dial :MM1 @22:32:43.11 delay 2 seconds @22:32:45.14 Adding MM1 @22:32:45.21 AS.seek 0.524ml @22:33:03.48 delay 25 seconds @22:33:28.50 ARV.dial :PP2 @22:33:28.87 Adding PP2 @22:33:28.88 delay 2 seconds @22:33:30.97 AS.seek 0.53ml @22:33:37.20 delay 10 seconds @22:33:47.22 ARV.dial :air2 @22:33:47.59 adding ARV air @22:33:47.60 delay 2 seconds @22:33:49.69 AS.seek 0.536ml @22:33:55.89 delay 5 seconds @22:34:00.91 ARV.dial :carrier @22:34:02.11 delay 7 seconds @22:34:09.20 AS.seek 0.594ml @22:35:07.91 delay 5 seconds @22:35:12.93 ATV.dial :ASV @22:35:13.33 delay 2 seconds @22:35:15.42 AS.seek 0.6ml @22:35:21.76 delay 4 seconds @22:35:25.78 ATV.dial ARV @22:35:26.33 delay 2 seconds @22:35:28.35 ARV.dial :carrier @22:35:28.37 delay 2 seconds @22:35:30.46 AS.seek 0.65ml @22:36:20.94 delay 4 seconds @22:36:24.96 ATV.dial PCR @22:36:25.55 ASV.dial :waste1 @22:36:25.56 ARV.dial :air1 @22:36:26.05 Positioning Slug by 0.324ml @22:36:26.14 AS.seek 0.326ml @22:41:52.14 delay 5 seconds @22:41:57.16 ATV.dial ARV @22:41:57.89 ASV.dial :PCRlock @22:41:58.27 delay 5 seconds @22:42:03.30 Running Ss Profile60C350 thermocycling @22:42:03.31 PCR.program Profile60C350 @22:42:03.46 Profile60C350 should complete in < 1:48:05 @22:42:03.47 PCR.run 2:20:06.25 @00:04:28.71CEST09-Oct-18 PCR.off @00:04:30.77 PCR for Ss completed -- closed /var/log/esp/Ss18oct0822h1150ml.pcr @00:04:30.84 Cleaning PCR @00:04:30.85 AFV.select :carrierSolution @00:04:30.97 delay 2 seconds @00:04:33.00 AS.reconfigure ASconfig @00:04:33.05 AS.seek 0.5ml @00:04:43.36 delay 1 second @00:04:44.37 AFV.select :holdingCoil @00:04:44.48 ASV.dial :waste1 @00:04:44.84 ATV.dial :air3 @00:04:46.05 AS.reconfigure ASslow1 @00:04:46.10 AS.seek 0.51ml @00:04:48.39 delay 2 seconds @00:04:50.41 ATV.dial :bleach @00:04:51.04 AS.seek 0.81ml @00:05:51.62 delay 3 seconds @00:05:54.64 ATV.dial :air3 @00:05:55.17 delay 3 seconds @00:05:58.26 AS.seek 0.835ml @00:06:03.56 delay 3 seconds @00:06:06.58 ATV.dial :bleach @00:06:07.18 AS.seek 0.82ml @00:06:10.48 delay 3 seconds @00:06:13.50 ARV.dial :waste @00:06:14.08 delay 3 seconds @00:06:17.10 ATV.dial :ARV @00:06:17.94 AS.reconfigure ASconfig @00:06:18.60 AS.seek 0.47ml @00:06:38.97 AFV.select :carrierSolution @00:06:39.09 delay 2 seconds @00:06:41.12 AS.seek 1ml @00:07:11.89 delay 1 second @00:07:12.90 AFV.select :holdingCoil @00:07:13.01 delay 1 second @00:07:14.04 AS.reconfigure ASfast @00:07:14.09 AS.seek 0ml @00:07:41.44 AFV.select :carrierSolution @00:07:41.56 delay 2 seconds @00:07:43.59 AS.reconfigure ASconfig @00:07:43.64 AS.seek 1ml @00:08:41.42 delay 1 second @00:08:42.43 AFV.select :holdingCoil @00:08:42.54 delay 1 second @00:08:43.57 AS.reconfigure ASfast @00:08:43.62 AS.seek 0ml @00:09:10.96 AFV.select :carrierSolution @00:09:11.08 delay 2 seconds @00:09:13.11 AS.reconfigure ASconfig @00:09:13.16 AS.seek 1ml @00:10:10.89 delay 1 second @00:10:11.90 AFV.select :holdingCoil @00:10:12.01 delay 1 second @00:10:13.04 AS.reconfigure ASfast @00:10:13.09 AS.seek 0ml @00:10:40.43 ARV.dial between carrier and air1 @00:10:40.92 ATV.dial :air3 @00:10:42.13 AS.reconfigure ASslow1 @00:10:42.18 AS.seek 0.01ml @00:10:44.53 delay 2 seconds @00:10:46.55 ATV.dial :bleach @00:10:47.16 AS.seek 0.81ml @00:13:28.31 delay 3 seconds @00:13:31.33 ATV.dial :air3 @00:13:31.86 delay 3 seconds @00:13:34.95 AS.seek 0.835ml @00:13:40.27 delay 3 seconds @00:13:43.29 ATV.dial :bleach @00:13:43.88 AS.seek 0.82ml @00:13:47.22 delay 3 seconds @00:13:50.24 ATV.dial PCR @00:13:50.63 AS.reconfigure ASconfig @00:13:50.68 AS.seek :empty @00:14:38.13 PCR.program :bleachProfile @00:14:38.20 PCR program should complete in < 2:45 @00:14:38.21 PCR.run 10:00 @00:17:49.11 PCR.off @00:17:49.14 AFV.select :carrierSolution @00:17:49.26 delay 2 seconds @00:17:51.29 AS.seek 1ml @00:18:49.11 delay 1 second @00:18:50.12 AFV.select :holdingCoil @00:18:50.23 delay 1 second @00:18:51.26 AS.reconfigure ASfast @00:18:51.31 AS.seek 0ml @00:19:18.64 delay 3 seconds @00:19:21.66 AFV.select :carrierSolution @00:19:21.77 delay 2 seconds @00:19:23.80 AS.reconfigure ASconfig @00:19:23.86 AS.seek 1ml @00:20:21.61 delay 1 second @00:20:22.62 AFV.select :holdingCoil @00:20:22.73 delay 1 second @00:20:23.76 AS.reconfigure ASfast @00:20:23.81 AS.seek 0ml @00:20:51.13 delay 3 seconds @00:20:54.15 AFV.select :carrierSolution @00:20:54.26 delay 2 seconds @00:20:56.29 AS.reconfigure ASconfig @00:20:56.34 AS.seek 1ml @00:21:54.14 delay 1 second @00:21:55.15 AFV.select :holdingCoil @00:21:55.26 delay 1 second @00:21:56.29 AS.reconfigure ASfast @00:21:56.34 AS.seek 0ml @00:22:23.69 delay 3 seconds @00:22:26.71 AFV.select :carrierSolution @00:22:26.82 delay 2 seconds @00:22:28.85 AS.reconfigure ASconfig @00:22:28.91 AS.seek 1ml @00:23:26.71 delay 1 second @00:23:27.72 AFV.select :holdingCoil @00:23:27.83 delay 1 second @00:23:28.86 AS.reconfigure ASfast @00:23:28.91 AS.seek 0ml @00:23:56.25 delay 3 seconds @00:23:59.27 AFV.select :carrierSolution @00:23:59.38 delay 2 seconds @00:24:01.41 AS.reconfigure ASconfig @00:24:01.46 AS.seek 1ml @00:24:59.24 delay 1 second @00:25:00.25 AFV.select :holdingCoil @00:25:00.36 delay 1 second @00:25:01.39 AS.reconfigure ASnorm @00:25:01.44 AS.seek 0ml @00:25:52.17 Setting up PCR for IPc -- writing /var/log/esp/IPc18oct0900h1150ml.pcr @00:25:52.19 Building PCR Slug from ASV with MM1 mix, PP1 primer @00:25:52.21 Priming with carrier ATV.dial ARV @00:25:53.50 ARV.dial :carrier @00:25:53.87 AS.seek 0.5ml @00:26:19.40 AFV.select :carrierSolution @00:26:19.52 delay 2 seconds @00:26:21.55 AS.reconfigure ASconfig @00:26:21.60 AS.seek 1ml @00:26:50.65 delay 1 second @00:26:51.67 AFV.select :holdingCoil @00:26:51.78 delay 1 second @00:26:52.80 ATV.dial :waste @00:26:54.44 AS.reconfigure ASnorm @00:26:54.50 AS.seek :empty @00:27:45.21 AFV.select :carrierSolution @00:27:45.33 delay 2 seconds @00:27:47.36 AS.reconfigure ASconfig @00:27:47.41 AS.seek 0.5ml @00:28:16.49 delay 1 second @00:28:17.50 AFV.select :holdingCoil @00:28:17.61 Building Slug @00:28:17.62 ATV.dial :ARV @00:28:19.23 delay 2 seconds @00:28:21.25 ARV.dial :air1 @00:28:21.64 delay 2 seconds @00:28:23.73 AS.reconfigure ASslow2 @00:28:23.78 AS.seek 0.506ml @00:28:30.01 delay 2 seconds @00:28:32.03 ARV.dial :MM1 @00:28:32.62 delay 2 seconds @00:28:34.65 Adding MM1 @00:28:34.72 AS.seek 0.524ml @00:28:53.07 delay 25 seconds @00:29:18.09 ARV.dial :PP1 @00:29:18.49 Adding PP1 @00:29:18.50 delay 2 seconds @00:29:20.59 AS.seek 0.53ml @00:29:26.94 delay 10 seconds @00:29:36.96 ARV.dial :air1 @00:29:37.33 adding ARV air @00:29:37.34 delay 2 seconds @00:29:39.43 AS.seek 0.536ml @00:29:45.76 delay 5 seconds @00:29:50.78 ARV.dial :carrier @00:29:51.18 delay 7 seconds @00:29:58.27 AS.seek 0.594ml @00:30:56.76 delay 5 seconds @00:31:01.78 ATV.dial :ASV @00:31:02.18 delay 2 seconds @00:31:04.27 AS.seek 0.6ml @00:31:10.54 delay 4 seconds @00:31:14.56 ATV.dial ARV @00:31:15.09 delay 2 seconds @00:31:17.11 ARV.dial :carrier @00:31:17.13 delay 2 seconds @00:31:19.22 AS.seek 0.65ml @00:32:09.79 delay 4 seconds @00:32:13.81 ATV.dial PCR @00:32:14.38 ASV.dial :waste1 @00:32:14.39 ARV.dial :air1 @00:32:14.77 Positioning Slug by 0.324ml @00:32:14.87 AS.seek 0.326ml @00:37:40.92 delay 5 seconds @00:37:45.94 ATV.dial ARV @00:37:46.67 ASV.dial :PCRlock @00:37:47.04 delay 5 seconds @00:37:52.07 Running IPc ProfileIPc thermocycling @00:37:52.08 PCR.program ProfileIPc @00:37:52.24 ProfileIPc should complete in < 1:50:49 @00:37:52.25 PCR.run 2:23:31.25 @02:02:10.91 PCR.off @02:02:13.98 PCR for IPc completed -- closed /var/log/esp/IPc18oct0900h1150ml.pcr @02:02:14.00 Full MFB Decontamination @02:02:14.01 Decon extraction column and decon AIV @02:02:14.04 SPE.hold 2:46:40,55C @02:02:14.08 ASV.dial :SPEdelivery @02:02:14.69 delay 20 seconds @02:02:34.72 AFV.select :carrierSolution @02:02:34.83 delay 2 seconds @02:02:36.86 AS.reconfigure ASconfig @02:02:36.91 AS.seek 0.5ml @02:02:47.21 delay 1 second @02:02:48.22 AFV.select :holdingCoil @02:02:48.33 ATV.dial :air1 @02:02:50.06 AS.reconfigure ASslow1 @02:02:50.11 AS.seek 0.52ml @02:02:54.39 ATV.dial :ASV @02:02:56.01 ASV.dial :SPErecovery @02:02:56.64 AS.seek 0.62ml @02:03:17.03 ATV.dial :waste @02:03:18.85 AS.reconfigure ASnorm @02:03:18.90 AS.seek :empty @02:03:50.53 AFV.select :holdingCoil @02:03:50.55 ATV.dial :waste @02:03:50.57 AS.reconfigure ASconfig @02:03:50.62 AS.seek 0ml @02:03:50.93 AFV.select :carrierSolution @02:03:51.05 delay 2 seconds @02:03:53.08 AS.seek 0.5ml @02:04:22.12 delay 1 second @02:04:23.13 AFV.select :holdingCoil @02:04:23.24 ATV.dial :air3 @02:04:23.92 AS.reconfigure ASslow1 @02:04:23.97 AS.seek 0.52ml @02:04:28.29 ATV.dial :air3 @02:04:28.38 AS.seek 0.53ml @02:04:30.68 delay 2 seconds @02:04:32.69 ATV.dial :bleach @02:04:33.17 AS.seek 0.71ml @02:05:09.65 delay 3 seconds @02:05:12.67 ATV.dial :air3 @02:05:13.21 delay 3 seconds @02:05:16.30 AS.seek 0.735ml @02:05:21.60 delay 3 seconds @02:05:24.62 ATV.dial :bleach @02:05:25.23 AS.seek 0.72ml @02:05:28.52 delay 3 seconds @02:05:31.55 ATV.dial ASV @02:05:32.65 ASV.dial :SPErecovery @02:05:32.73 AS.seek 0.365ml @02:06:44.39 ATV.dial :waste @02:06:46.10 AS.reconfigure ASnorm @02:06:46.15 AS.seek :empty @02:07:04.92 ATV.dial :air3 @02:07:05.59 AS.reconfigure ASslow1 @02:07:05.64 AS.seek 0.009ml @02:07:07.95 delay 2 seconds @02:07:09.97 ATV.dial :bleach @02:07:10.44 AS.seek 0.579ml @02:09:05.34 delay 3 seconds @02:09:08.36 ATV.dial :air3 @02:09:08.88 delay 3 seconds @02:09:11.97 AS.seek 0.604ml @02:09:17.27 delay 3 seconds @02:09:20.29 ATV.dial :bleach @02:09:20.89 AS.seek 0.589ml @02:09:24.20 delay 3 seconds @02:09:27.22 ATV.dial :ASV @02:09:28.18 ASV.dial :wasteR @02:09:28.57 AS.seek 0ml @02:11:27.31 delay 1:00 @02:12:27.33 ATV.dial ASV @02:12:27.35 AFV.select :carrierSolution @02:12:27.46 delay 2 seconds @02:12:29.49 AS.reconfigure ASconfig @02:12:29.54 AS.seek 1ml @02:13:27.34 delay 1 second @02:13:28.35 AFV.select :holdingCoil @02:13:28.46 delay 1 second @02:13:29.48 ASV.dial :wasteR @02:13:30.09 AS.reconfigure ASslow1 @02:13:30.17 AS.seek 0ml @02:16:51.57 delay 10 seconds @02:17:01.60 ATV.dial :air3 @02:17:03.00 AS.seek 0.01ml @02:17:05.29 delay 2 seconds @02:17:07.30 ATV.dial :bleach @02:17:07.91 AS.seek 0.58ml @02:19:02.82 delay 3 seconds @02:19:05.84 ATV.dial :air3 @02:19:06.37 delay 3 seconds @02:19:09.46 AS.seek 0.605ml @02:19:14.77 delay 3 seconds @02:19:17.79 ATV.dial :bleach @02:19:18.39 AS.seek 0.59ml @02:19:21.67 delay 3 seconds @02:19:24.70 ATV.dial ASV @02:19:25.65 ASV.dial :SPEdelivery @02:19:26.01 AS.seek 0ml @02:21:24.86 delay 1:00 @02:22:24.88 AFV.select :carrierSolution @02:22:24.99 delay 2 seconds @02:22:27.02 AS.reconfigure ASconfig @02:22:27.07 AS.seek 1ml @02:23:24.87 delay 1 second @02:23:25.89 AFV.select :holdingCoil @02:23:26.00 delay 1 second @02:23:27.02 ATV.dial ASV @02:23:27.04 ASV.dial :SPEdelivery @02:23:27.06 AS.reconfigure ASslow1 @02:23:27.10 AS.seek 0ml @02:26:48.51 delay 10 seconds @02:26:58.53 ATV.dial :air2 @02:26:59.31 AS.reconfigure ASslow0 @02:26:59.37 AS.seek 0.8ml @02:28:20.17 delay 3 seconds @02:28:23.19 ATV.dial :ASV @02:28:24.14 ASV.dial :SPEdelivery @02:28:24.16 AS.reconfigure ASslow1 @02:28:24.21 AS.seek 0.03ml @02:30:59.30 delay 20 seconds @02:31:19.33 ATV.dial :waste,via: :air3 @02:31:20.92 AS.reconfigure ASconfig @02:31:20.97 AS.seek :empty @02:31:23.05 SPE.coast @02:31:23.14 Removing Bleach from Mixing Coil @02:31:23.15 AFV.select :carrierSolution @02:31:23.26 delay 2 seconds @02:31:25.29 AS.seek 0.5ml @02:31:54.41 delay 1 second @02:31:55.42 AFV.select :holdingCoil @02:31:55.53 ATV.dial :ASV @02:31:57.11 ASV.dial :SPErecovery @02:31:57.74 AS.reconfigure ASslow1 @02:31:57.79 AS.seek 0.73ml @02:32:44.33 ASV.dial :wasteD @02:32:44.89 AS.reconfigure ASnorm @02:32:44.95 AS.seek 0ml @02:33:22.05 AFV.select :carrierSolution @02:33:22.17 delay 2 seconds @02:33:24.20 AS.reconfigure ASconfig @02:33:24.25 AS.seek 0.5ml @02:33:53.31 delay 1 second @02:33:54.32 AFV.select :holdingCoil @02:33:54.43 ATV.dial ASV @02:33:54.44 ASV.dial :SPErecovery @02:33:55.07 AS.reconfigure ASslow0 @02:33:55.12 AS.seek 0.3ml @02:34:15.52 ATV.dial :air2 @02:34:16.60 AS.reconfigure ASslow1 @02:34:16.65 AS.seek 0.32ml @02:34:20.99 ATV.dial ASV @02:34:22.09 AS.reconfigure ASslow0 @02:34:22.14 AS.seek 0.57ml @02:34:47.62 ASV.dial :wasteD @02:34:48.27 AS.reconfigure ASnorm @02:34:48.32 AS.seek 0ml @02:35:17.40 AFV.select :carrierSolution @02:35:17.52 delay 2 seconds @02:35:19.55 AS.reconfigure ASconfig @02:35:19.60 AS.seek 0.5ml @02:35:48.69 delay 1 second @02:35:49.70 AFV.select :holdingCoil @02:35:49.81 ATV.dial ASV @02:35:49.82 ASV.dial :SPErecovery @02:35:50.45 AS.reconfigure ASslow0 @02:35:50.50 AS.seek 0.3ml @02:36:10.96 ATV.dial :air2 @02:36:11.95 AS.reconfigure ASslow1 @02:36:12.00 AS.seek 0.32ml @02:36:16.36 ATV.dial ASV @02:36:17.35 AS.reconfigure ASslow0 @02:36:17.40 AS.seek 0.57ml @02:36:42.88 ASV.dial :wasteD @02:36:43.45 AS.reconfigure ASnorm @02:36:43.51 AS.seek 0ml @02:37:12.57 ATV.dial :air2 @02:37:13.50 AS.reconfigure ASslow0 @02:37:13.56 AS.seek 0.3ml @02:37:44.10 ATV.dial :ASV @02:37:45.02 ASV.dial :wasteD @02:37:45.04 AS.reconfigure ASconfig @02:37:45.09 AS.seek 0.05ml @02:37:59.77 ATV.dial :waste @02:38:01.51 AS.seek 0ml @02:38:04.68 ASV.dial :SPEdelivery @02:38:05.63 deconLysateINT @02:38:05.64 ATV.dial :air3 @02:38:06.31 AS.reconfigure ASslow1 @02:38:06.36 AS.seek 0.01ml @02:38:08.80 delay 2 seconds @02:38:10.82 ATV.dial :bleach @02:38:11.38 AS.seek 0.96ml @02:41:22.69 delay 3 seconds @02:41:25.71 ATV.dial :air3 @02:41:26.24 delay 3 seconds @02:41:29.33 AS.seek 0.985ml @02:41:34.65 delay 3 seconds @02:41:37.67 ATV.dial :bleach @02:41:38.28 AS.seek 0.97ml @02:41:41.55 delay 3 seconds @02:41:44.58 ATV.dial AIV @02:41:45.83 AIV.dial :coilToMFB @02:41:45.84 AWV.select :waste @02:41:45.86 AFV.select :carrierSolution @02:41:45.97 delay 2 seconds @02:41:48.00 AS.reconfigure ASconfig @02:41:48.05 AS.seek 1ml @02:41:50.08 delay 1 second @02:41:51.09 AFV.select :holdingCoil @02:41:51.20 delay 1 second @02:41:52.23 AS.reconfigure ASslow0 @02:41:52.27 AS.seek :empty @02:43:33.21 AFV.select :carrierSolution @02:43:33.33 delay 2 seconds @02:43:35.36 AS.reconfigure ASconfig @02:43:35.41 AS.seek 1ml @02:44:33.22 delay 1 second @02:44:34.24 AFV.select :holdingCoil @02:44:34.35 delay 1 second @02:44:35.38 AS.reconfigure ASslow0 @02:44:35.42 AS.seek :empty @02:46:16.42 AFV.select :carrierSolution @02:46:16.54 delay 2 seconds @02:46:18.57 AS.reconfigure ASconfig @02:46:18.62 AS.seek 1ml @02:47:16.41 delay 1 second @02:47:17.43 AFV.select :holdingCoil @02:47:17.54 delay 1 second @02:47:18.57 AS.reconfigure ASslow0 @02:47:18.62 AS.seek :empty @02:48:59.54 ATV.dial :air2 @02:49:00.20 AS.reconfigure ASslow1 @02:49:00.25 AS.seek 0.009ml @02:49:02.60 AS.reconfigure ASslow0 @02:49:02.65 AS.seek 1ml @02:50:42.59 ATV.dial AIV @02:50:43.34 AS.seek 0.02ml @02:52:22.23 ATV.dial :waste @02:52:23.87 AS.seek 0ml @02:52:26.17 AWV.select :air @02:52:26.34 Cleaning PCR @02:52:26.35 AFV.select :carrierSolution @02:52:26.46 delay 2 seconds @02:52:28.49 AS.reconfigure ASconfig @02:52:28.54 AS.seek 0.5ml @02:52:57.57 delay 1 second @02:52:58.58 AFV.select :holdingCoil @02:52:58.69 ASV.dial :waste1 @02:52:59.04 ATV.dial :air3 @02:52:59.70 AS.reconfigure ASslow1 @02:52:59.75 AS.seek 0.51ml @02:53:02.02 delay 2 seconds @02:53:04.04 ATV.dial :bleach @02:53:04.50 AS.seek 0.81ml @02:54:05.10 delay 3 seconds @02:54:08.12 ATV.dial :air3 @02:54:08.65 delay 3 seconds @02:54:11.74 AS.seek 0.835ml @02:54:17.02 delay 3 seconds @02:54:20.66 ATV.dial :bleach @02:54:21.26 AS.seek 0.82ml @02:54:24.58 delay 3 seconds @02:54:27.60 ARV.dial :waste @02:54:28.19 delay 3 seconds @02:54:31.21 ATV.dial :ARV @02:54:32.06 AS.reconfigure ASconfig @02:54:32.11 AS.seek 0.47ml @02:54:52.50 AFV.select :carrierSolution @02:54:52.62 delay 2 seconds @02:54:54.65 AS.seek 1ml @02:55:25.43 delay 1 second @02:55:26.44 AFV.select :holdingCoil @02:55:26.55 delay 1 second @02:55:27.58 AS.reconfigure ASfast @02:55:27.63 AS.seek 0ml @02:55:54.98 AFV.select :carrierSolution @02:55:55.10 delay 2 seconds @02:55:57.13 AS.reconfigure ASconfig @02:55:57.18 AS.seek 1ml @02:56:54.93 delay 1 second @02:56:55.94 AFV.select :holdingCoil @02:56:56.05 delay 1 second @02:56:57.08 AS.reconfigure ASfast @02:56:57.13 AS.seek 0ml @02:57:24.48 AFV.select :carrierSolution @02:57:24.60 delay 2 seconds @02:57:26.63 AS.reconfigure ASconfig @02:57:26.68 AS.seek 1ml @02:58:24.46 delay 1 second @02:58:25.47 AFV.select :holdingCoil @02:58:25.58 delay 1 second @02:58:26.61 AS.reconfigure ASfast @02:58:26.66 AS.seek 0ml @02:58:54.01 ARV.dial between carrier and air1 @02:58:54.49 ATV.dial :air3 @02:58:55.70 AS.reconfigure ASslow1 @02:58:55.76 AS.seek 0.01ml @02:58:58.10 delay 2 seconds @02:59:00.11 ATV.dial :bleach @02:59:00.72 AS.seek 0.81ml @03:01:41.91 delay 3 seconds @03:01:44.93 ATV.dial :air3 @03:01:45.46 delay 3 seconds @03:01:48.55 AS.seek 0.835ml @03:01:53.85 delay 3 seconds @03:01:56.87 ATV.dial :bleach @03:01:57.47 AS.seek 0.82ml @03:02:00.79 delay 3 seconds @03:02:03.81 ATV.dial PCR @03:02:04.20 AS.reconfigure ASconfig @03:02:04.25 AS.seek :empty @03:02:51.73 PCR.program :bleachProfile @03:02:51.79 PCR program should complete in < 2:45 @03:02:51.80 PCR.run 10:00 @03:06:11.80 PCR.off @03:06:11.82 AFV.select :carrierSolution @03:06:11.93 delay 2 seconds @03:06:13.96 AS.seek 1ml @03:07:11.81 delay 1 second @03:07:12.82 AFV.select :holdingCoil @03:07:13.04 delay 1 second @03:07:14.07 AS.reconfigure ASfast @03:07:14.12 AS.seek 0ml @03:07:41.46 delay 3 seconds @03:07:44.48 AFV.select :carrierSolution @03:07:44.59 delay 2 seconds @03:07:46.62 AS.reconfigure ASconfig @03:07:46.68 AS.seek 1ml @03:08:44.46 delay 1 second @03:08:45.47 AFV.select :holdingCoil @03:08:45.58 delay 1 second @03:08:46.61 AS.reconfigure ASfast @03:08:46.66 AS.seek 0ml @03:09:13.98 delay 3 seconds @03:09:17.00 AFV.select :carrierSolution @03:09:17.11 delay 2 seconds @03:09:19.14 AS.reconfigure ASconfig @03:09:19.20 AS.seek 1ml @03:10:16.98 delay 1 second @03:10:18.00 AFV.select :holdingCoil @03:10:18.11 delay 1 second @03:10:19.14 AS.reconfigure ASfast @03:10:19.19 AS.seek 0ml @03:10:46.54 delay 3 seconds @03:10:49.56 AFV.select :carrierSolution @03:10:49.67 delay 2 seconds @03:10:51.70 AS.reconfigure ASconfig @03:10:51.75 AS.seek 1ml @03:11:49.53 delay 1 second @03:11:50.54 AFV.select :holdingCoil @03:11:50.65 delay 1 second @03:11:51.68 AS.reconfigure ASfast @03:11:51.73 AS.seek 0ml @03:12:19.78 delay 3 seconds @03:12:22.80 AFV.select :carrierSolution @03:12:22.91 delay 2 seconds @03:12:24.96 AS.reconfigure ASconfig @03:12:25.00 AS.seek 1ml @03:13:22.79 delay 1 second @03:13:23.80 AFV.select :holdingCoil @03:13:23.91 delay 1 second @03:13:24.94 AS.reconfigure ASnorm @03:13:24.99 AS.seek 0ml @03:14:15.66 AFV.select :carrierSolution @03:14:15.77 delay 2 seconds @03:14:17.80 AS.reconfigure ASconfig @03:14:17.85 AS.seek 1ml @03:15:15.61 delay 1 second @03:15:16.63 AFV.select :holdingCoil @03:15:16.74 delay 1 second @03:15:17.76 ATV.dial :waste @03:15:18.90 AS.reconfigure ASnorm @03:15:18.96 AS.seek 0.5ml @03:15:44.50 ATV.dial :waste2 @03:15:45.69 AS.seek 0ml @03:16:11.18 AS.coast @03:16:11.23 SS.coast @03:16:11.27 CC.coast @03:16:11.31 CS.coast @03:16:11.35 PC.coast @03:16:11.39 PS.coast @03:16:11.43 Forearm.coast @03:16:11.47 Elbow.coast @03:16:11.87 Gate.power :core,:OFF @03:16:11.89 Gate.power :raw,:OFF @03:16:11.90 Shutdown @03:16:11.91 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @03:16:52.07 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @03:16:52.08 Retry #1 of 10 after 10:00 @03:26:52.12 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @07:41:43.05 Session Begun @07:42:24.21 -> ESP.wakeup @07:42:24.28 Powering core bus @07:42:29.19 Gate.power :raw,:ON @07:42:29.88 SPE.coast @07:42:29.98 SPE.configure SPEconfig @07:42:30.10 AS.configure ASconfig @07:42:30.23 SS.configure SSconfig @07:42:30.35 CC.configure CCconfig @07:42:30.48 CS.configure CSconfig @07:42:30.61 PC.configure PCconfig @07:42:30.73 PS.configure PSconfig @07:42:30.88 Forearm.configure LooseForearm @07:42:31.00 Elbow.configure LooseElbow @07:42:31.15 SC.configure SCloose @07:42:31.27 SE.configure SEconfig @07:42:31.34 CH.coast @07:42:31.42 CH.configure CHconfig @07:42:31.48 PH.coast @07:42:31.57 PH.configure PHconfig @07:42:31.63 PCR.off @07:42:31.68 PCR.pidtemp= 50,0.1,0,300,25,25 @07:42:31.74 PCR.caltemp= 2.7073,0.0373,0 @07:42:31.78 All dwarf controllers running firmware version 3.75 @07:42:31.91 Valve.reset @07:42:32.10 PCR.off @07:42:32.15 PCR.pidtemp= 50,0.1,0,300,25,25 @07:42:32.20 PCR.caltemp= 2.7073,0.0373,0 @07:42:32.39 Intake.select :close @07:42:32.43 Exhaust.select :close @07:42:32.48 CTV.dial :air @07:42:32.61 PSV.dial :air @07:42:32.65 CSV.dial :waste1 @07:42:32.87 SSV.dial :spit,avoiding: :exhaust @07:42:32.90 Forearm.reconfigure LooseForearm @07:42:33.02 Forearm.home.seek :retract @07:42:33.21 PS.reconfigure PSconfig @07:42:33.28 PS.home.seek 0ml @07:42:33.76 Hand.close @07:42:33.80 Elbow.jog 1500 @07:42:33.98 CS.reconfigure CSfast @07:42:34.62 SS.reconfigure SSconfig @07:42:34.72 CS.home.seek 0ml @07:42:34.77 SS.home.seek 0ml @07:42:34.92 Elbow.reconfigure LooseElbow @07:42:35.00 Elbow.home.seek :clear @07:42:38.46 SE.reconfigure SEconfig @07:42:38.54 SE.home.seek :down @07:42:39.31 SC.reconfigure SCsloppy @07:42:39.38 SC.home.seek :initial @07:42:42.42 PS.coast @07:42:43.28 Forearm.seek :retract @07:42:43.40 Elbow.seek :hammer @07:42:44.73 Forearm.reconfigure TightForearm @07:42:44.82 Forearm.seek 5 + 350 counts @07:42:45.90 Forearm.seek :retract @07:42:47.19 CC.reconfigure CCconfig @07:42:47.22 PC.reconfigure PCconfig @07:42:47.29 CC.home.seek :open @07:42:47.40 PC.home.seek :open @07:43:00.05 -> can @07:43:12.27 -> MFB.ready? @07:43:17.35 -> MFB.ready! @07:43:17.52 Readying the MFB @07:43:17.53 Homing MFB Syringe @07:43:17.55 AFV.select :holdingCoil @07:43:17.74 ATV.dial :air2 @07:43:18.28 AS.reconfigure ASconfig @07:43:18.36 AS.home.seek :home @07:43:23.90 AFV.select :carrierSolution @07:43:24.04 delay 2 seconds @07:43:26.07 AS.seek 1ml @07:44:19.02 delay 1 second @07:44:20.03 AFV.select :holdingCoil @07:44:20.16 delay 1 second @07:44:21.19 ATV.dial :waste @07:44:22.20 AS.reconfigure ASnorm @07:44:22.28 AS.home.seek :empty @07:45:14.86 ATV.dial :waste @07:45:14.88 ARV.dial between carrier and air1 @07:45:14.99 ASV.dial :waste1 @07:48:39.34 Session Begun @07:48:51.18 -> showlog 20 @07:49:31.21 -> MFB.ready? @07:50:06.35 Session Ended @07:50:44.14 -> showlog 20 @07:51:41.21 -> ATV @07:52:31.56 -> ATV.ARV @07:52:31.57 ATV.dial ARV @07:52:38.09 -> ARV.air3 @07:52:38.10 ARV.dial :air3 @07:52:59.68 -> AS.pull 0.2, ASslow1 @07:52:59.79 AS.reconfigure ASslow1 @07:52:59.86 AS.seek 0.199ml @07:53:49.02 -> ARV.PP5 @07:53:49.04 ARV.dial :PP5 @07:54:06.40 -> AS.pull 0.075, ASslow1 @07:54:06.51 AS.seek 0.274ml @07:54:28.08 -> ARV.air3 @07:54:28.10 ARV.dial :air3 @07:54:55.29 -> AS.pull 0.2, ASslow1 @07:54:55.40 AS.seek 0.474ml @07:55:49.95 -> ATV.waste @07:55:49.97 ATV.dial :waste @07:55:55.24 -> AS.empty @07:55:55.27 AS.reconfigure ASconfig @07:55:55.36 AS.seek :empty @07:56:36.40 -> pcrDecon @07:56:36.50 Cleaning PCR @07:56:36.51 AFV.select :carrierSolution @07:56:36.66 delay 2 seconds @07:56:38.69 AS.seek 0.5ml @07:57:07.78 delay 1 second @07:57:08.79 AFV.select :holdingCoil @07:57:08.93 ASV.dial :waste1 @07:57:08.95 ATV.dial :air3 @07:57:09.87 AS.reconfigure ASslow1 @07:57:09.95 AS.seek 0.51ml @07:57:12.24 delay 2 seconds @07:57:14.26 ATV.dial :bleach @07:57:14.73 AS.seek 0.81ml @07:58:15.35 delay 3 seconds @07:58:18.37 ATV.dial :air3 @07:58:18.91 delay 3 seconds @07:58:22.00 AS.seek 0.835ml @07:58:27.41 delay 3 seconds @07:58:30.43 ATV.dial :bleach @07:58:31.04 AS.seek 0.82ml @07:58:34.38 delay 3 seconds @07:58:37.40 ARV.dial :waste @07:58:38.39 delay 3 seconds @07:58:41.41 ATV.dial :ARV @07:58:42.28 AS.reconfigure ASconfig @07:58:42.35 AS.seek 0.47ml @07:59:02.78 AFV.select :carrierSolution @07:59:02.92 delay 2 seconds @07:59:04.95 AS.seek 1ml @07:59:35.80 delay 1 second @07:59:36.81 AFV.select :holdingCoil @07:59:36.94 delay 1 second @07:59:37.97 AS.reconfigure ASfast @07:59:38.04 AS.seek 0ml @08:00:05.38 AFV.select :carrierSolution @08:00:05.52 delay 2 seconds @08:00:07.55 AS.reconfigure ASconfig @08:00:07.63 AS.seek 1ml @08:01:05.44 delay 1 second @08:01:06.46 AFV.select :holdingCoil @08:01:06.59 delay 1 second @08:01:07.62 AS.reconfigure ASfast @08:01:07.69 AS.seek 0ml @08:01:35.04 AFV.select :carrierSolution @08:01:35.19 delay 2 seconds @08:01:37.22 AS.reconfigure ASconfig @08:01:37.29 AS.seek 1ml @08:02:35.08 delay 1 second @08:02:36.09 AFV.select :holdingCoil @08:02:36.22 delay 1 second @08:02:37.25 AS.reconfigure ASfast @08:02:37.32 AS.seek 0ml @08:03:04.67 ARV.dial between carrier and air1 @08:03:05.18 ATV.dial :air3 @08:03:06.40 AS.reconfigure ASslow1 @08:03:06.47 AS.seek 0.01ml @08:03:08.83 delay 2 seconds @08:03:10.84 ATV.dial :bleach @08:03:11.45 AS.seek 0.81ml @08:05:52.62 delay 3 seconds @08:05:55.64 ATV.dial :air3 @08:05:56.19 delay 3 seconds @08:05:59.28 AS.seek 0.835ml @08:06:04.64 delay 3 seconds @08:06:07.66 ATV.dial :bleach @08:06:08.28 AS.seek 0.82ml @08:06:11.55 delay 3 seconds @08:06:14.57 ATV.dial PCR @08:06:14.98 AS.reconfigure ASconfig @08:06:15.05 AS.seek :empty @08:07:02.51 PCR.program :bleachProfile @08:07:02.59 PCR program should complete in < 2:45 @08:07:02.60 PCR.run 10:00 @08:10:34.75 PCR.off @08:10:34.79 AFV.select :carrierSolution @08:10:34.93 delay 2 seconds @08:10:36.96 AS.seek 1ml @08:11:34.84 delay 1 second @08:11:35.85 AFV.select :holdingCoil @08:11:36.00 delay 1 second @08:11:37.03 AS.reconfigure ASfast @08:11:37.10 AS.seek 0ml @08:12:04.48 delay 3 seconds @08:12:07.50 AFV.select :carrierSolution @08:12:07.64 delay 2 seconds @08:12:09.67 AS.reconfigure ASconfig @08:12:09.76 AS.seek 1ml @08:13:07.57 delay 1 second @08:13:08.59 AFV.select :holdingCoil @08:13:08.73 delay 1 second @08:13:09.76 AS.reconfigure ASfast @08:13:09.83 AS.seek 0ml @08:13:37.18 delay 3 seconds @08:13:40.20 AFV.select :carrierSolution @08:13:40.34 delay 2 seconds @08:13:42.37 AS.reconfigure ASconfig @08:13:42.45 AS.seek 1ml @08:14:40.29 delay 1 second @08:14:41.31 AFV.select :holdingCoil @08:14:41.44 delay 1 second @08:14:42.47 AS.reconfigure ASfast @08:14:42.54 AS.seek 0ml @08:15:09.90 delay 3 seconds @08:15:12.92 AFV.select :carrierSolution @08:15:13.05 delay 2 seconds @08:15:15.08 AS.reconfigure ASconfig @08:15:15.15 AS.seek 1ml @08:16:12.95 delay 1 second @08:16:13.97 AFV.select :holdingCoil @08:16:14.10 delay 1 second @08:16:15.13 AS.reconfigure ASfast @08:16:15.20 AS.seek 0ml @08:16:42.57 delay 3 seconds @08:16:45.59 AFV.select :carrierSolution @08:16:45.72 delay 2 seconds @08:16:47.75 AS.reconfigure ASconfig @08:16:47.82 AS.seek 1ml @08:17:45.62 delay 1 second @08:17:46.63 AFV.select :holdingCoil @08:17:46.77 delay 1 second @08:17:47.80 AS.reconfigure ASnorm @08:17:47.87 AS.seek 0ml @08:21:22.94 -> can @08:21:35.17 -> ESP.shutdown @08:21:35.21 AS.coast @08:21:35.24 Asleep / Shutdown @08:21:35.31 SS.coast @08:21:35.38 CC.coast @08:21:35.44 CS.coast @08:21:35.50 PC.coast @08:21:35.56 Forearm.coast @08:21:36.18 Elbow.coast @08:21:36.24 SC.coast @08:21:36.31 SE.coast @08:21:36.72 Gate.power :core,:OFF @08:21:36.74 Gate.power :raw,:OFF @08:21:36.76 Shutdown @08:22:29.96 -> exit @08:22:29.98 Session Ended @08:43:14.24 Session Begun @08:44:12.34 -> $dilmfb[:dilution] = 0.1 @08:46:09.59 -> can @08:51:18.21 -> exit @08:51:18.23 Session Ended @14:00:00.05
Powering core bus @14:00:04.83 Gate.power :raw,:ON @14:00:05.62 SPE.coast @14:00:05.69 SPE.configure SPEconfig @14:00:05.79 AS.configure ASconfig @14:00:05.90 SS.configure SSconfig @14:00:06.01 CC.configure CCconfig @14:00:06.11 CS.configure CSconfig @14:00:06.22 PC.configure PCconfig @14:00:06.33 PS.configure PSconfig @14:00:06.46 Forearm.configure LooseForearm @14:00:06.56 Elbow.configure LooseElbow @14:00:06.70 SC.configure SCloose @14:00:06.80 SE.configure SEconfig @14:00:06.85 CH.coast @14:00:06.91 CH.configure CHconfig @14:00:06.95 PH.coast @14:00:07.01 PH.configure PHconfig @14:00:07.05 PCR.off @14:00:07.08 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:07.11 PCR.caltemp= 2.7073,0.0373,0 @14:00:07.14 All dwarf controllers running firmware version 3.75 @14:00:07.26 Valve.reset @14:00:07.64 PCR.off @14:00:07.70 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:07.77 PCR.caltemp= 2.7073,0.0373,0 @14:00:08.36 Intake.select :close @14:00:08.42 Exhaust.select :close @14:00:08.43 CTV.dial :air @14:00:08.52 PSV.dial :air @14:00:08.56 CSV.dial :waste1 @14:00:08.75 SSV.dial :spit,avoiding: :exhaust @14:00:08.79 PS.reconfigure PSconfig @14:00:08.81
Forearm.reconfigure LooseForearm @14:00:08.95 PS.home.seek 0ml @14:00:08.98
Forearm.home.seek :retract @14:00:09.17 CS.reconfigure CSfast @14:00:09.20 SS.reconfigure SSconfig @14:00:09.30 CS.home.seek 0ml @14:00:09.33 SS.home.seek 0ml @14:00:09.77 Hand.close @14:00:09.84
Elbow.jog 1500 @14:00:10.28 Elbow.reconfigure LooseElbow @14:00:10.33 Elbow.home.seek :clear @14:00:15.01 SE.reconfigure SEconfig @14:00:15.05 SE.home.seek :down @14:00:15.77 SC.reconfigure SCsloppy @14:00:15.82 SC.home.seek :initial @14:00:18.05 PS.coast @14:00:19.78
Forearm.seek :retract @14:00:19.86 Elbow.seek :hammer @14:00:21.12 Forearm.reconfigure TightForearm @14:00:21.17 Forearm.seek 5 + 350 counts @14:00:22.24 Forearm.seek :retract @14:00:23.45 PC.reconfigure PCconfig @14:00:23.46
CC.reconfigure CCconfig @14:00:23.55 PC.home.seek :open @14:00:23.58
CC.home.seek :open @14:00:27.56 Phase [phasewithdil] @14:00:27.58 GoodNews.email "At 14:00:27 began in tube 3: phasewithdil\n/home/esp/esp2/mission/18tundeployv2dil.rb:118\n{1 => 22, 2 => 5, 3 => 17}\nCan@14:00:27, 13.7C, 70% humidity, 14.3psia, 13.402V, 0.815A, 0.815A avg, 10.92W",Subject: "Started 18tundeployv2dil phase phasewithdil" @14:00:27.60CEST09-Oct-18
==================> STARTED Superphasewdil (phase #6) <=== @14:00:27.62 Starting initialPurge @14:00:27.65 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:00:27.73 Hand.open @14:00:28.46 Elbow.reconfigure TightElbow @14:00:28.51 Elbow.seek FlushPuck::Garage @14:00:30.35 Elbow.reconfigure LooseElbow @14:00:30.45 Forearm.reconfigure LooseForearm @14:00:30.50 Forearm.seek :garage @14:00:31.53 Hand.close @14:00:32.35 Forearm.seek :retract @14:00:33.34 Elbow.reconfigure TightElbow @14:00:33.39 Elbow.seek CC @14:00:36.29 Elbow.reconfigure LooseElbow @14:00:36.40 Forearm.reconfigure TightForearm @14:00:36.47 Forearm.seek CC @14:00:37.76 CC.seek :closed @14:00:42.08 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.12 @14:00:42.18 Hand.open @14:00:42.92 Forearm.reconfigure LooseForearm @14:00:42.96 Forearm.seek :retract @14:00:43.97 Flushing the Collection Valve::Manifold @14:00:44.13 CSV.dial CSR @14:00:45.55 Pulling 5ml of CSR.flush @14:00:45.56 CSR.select :flush @14:00:45.69 CS.reconfigure CSconfig @14:00:45.73 CS.seek 5ml @14:00:59.44 delay 5 seconds @14:01:04.46 CSR.select :air @14:01:04.63 CS.reconfigure CSfast @14:01:04.68 CS.seek 7.5ml @14:01:11.46 CSV.dial :waste @14:01:12.80 CS.reconfigure CSconfig @14:01:12.85 CS.seek 0ml @14:01:33.06 Purging external Intake @14:01:33.19 CSV.dial :flush @14:01:33.67 CS.reconfigure CSfast @14:01:33.72 CS.seek 10ml @14:01:57.57 delay 5 seconds @14:02:02.60 CSV.dial CBV @14:02:02.64
CBV.dial CSV @14:02:03.42 CTV.dial :intake @14:02:04.21 Intake.select :open @14:02:04.33 CS.seek 0ml @14:02:29.21 Intake.select :close @14:02:29.33 CTV.dial :air @14:02:30.11 Flushing upper manifold @14:02:30.24 CSV.dial :flush @14:02:31.56 CS.seek 5ml @14:02:44.10 delay 5 seconds @14:02:49.12 CSV.dial :air @14:02:49.68 CS.seek 7ml @14:02:55.25 CSV.dial CBV @14:02:55.29
CBV.dial CSV @14:02:56.36 CTV.dial :air @14:02:56.38 CS.seek 0ml @14:03:13.35 delay 7 seconds @14:03:20.38 CS.seek 3ml @14:03:28.34 CSV.dial :waste @14:03:28.84 CS.seek 0ml @14:03:36.94 CSV.dial :flush @14:03:37.42 CS.seek 3ml @14:03:45.32 delay 5 seconds @14:03:50.34 CSV.dial :air @14:03:50.90 CS.seek 5ml @14:03:56.46 CSV.dial :air @14:03:56.49 CS.seek 0ml @14:04:08.97 delay 7 seconds @14:04:16.00 CS.seek 3ml @14:04:23.90 CSV.dial :waste @14:04:24.68 CS.seek 0ml @14:04:32.57 Garaging FlushPuck in Collection Clamp @14:04:32.65 Elbow.reconfigure TightElbow @14:04:32.70 Elbow.seek CC @14:04:32.94 Elbow.reconfigure LooseElbow @14:04:33.05 Forearm.seek CC @14:04:34.04 Hand.close @14:04:34.71 CC.seek :open @14:04:39.07 Forearm.seek :retract @14:04:40.08 Elbow.reconfigure TightElbow @14:04:40.13 Elbow.seek :garage @14:04:43.18 Elbow.reconfigure LooseElbow @14:04:43.30 Forearm.reconfigure TightForearm @14:04:43.35 Forearm.seek :garage @14:04:44.50 Hand.open @14:04:45.15 Forearm.reconfigure LooseForearm @14:04:45.19 Forearm.seek :retract @14:04:46.28 Starting mfb @14:04:46.30 pcrBAC sampling at most 1500ml, wcr at most 1500ml @14:04:46.38 BACfans.email "Started mfb in tube 3 at 14:04:46CEST09-Oct-18\n{1 => 22, 2 => 5, 3 => 17}\nCan@14:00:27, 13.7C, 70% humidity, 14.3psia, 13.402V, 0.815A, 0.815A avg, 10.92W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @14:04:46.41
Loading sh1 puck from tube 3 into Collection Clamp @14:04:46.53 Readying the MFB @14:04:46.59 Homing MFB Syringe @14:04:46.60 AFV.select :holdingCoil @14:04:46.84 ATV.dial :air2 @14:04:46.87
SC.reconfigure SCtight @14:04:46.97 SC.seek 3 @14:04:48.36 AS.reconfigure ASconfig @14:04:48.42 AS.home.seek :home @14:04:49.01
SC.reconfigure SCloose @14:04:49.24 Elbow.reconfigure TightElbow @14:04:49.29 Elbow.seek 3 @14:04:51.46 Forearm.reconfigure TightForearm @14:04:51.52 Forearm.seek 3 + 700 counts @14:04:53.08 SE.reconfigure SEpick @14:04:53.17 SE.seek beamBreak @14:04:53.96 AFV.select :carrierSolution @14:04:54.13 delay 2 seconds @14:04:54.74
SE.configure SEconfig @14:04:54.92 Elbow.seek 3 @14:04:55.21 Forearm.seek 3 + 300 counts @14:04:55.49 Forearm.reconfigure LooseForearm @14:04:55.55 Hand.close @14:04:56.23 SE.seek :down @14:04:56.37 AS.seek 1ml @14:04:56.53
Forearm.seek :retract @14:04:57.51 Elbow.seek CC @14:04:57.58 SE.coast @14:04:59.17
Elbow.reconfigure LooseElbow @14:04:59.29 Forearm.reconfigure TightForearm @14:04:59.34 Forearm.seek CC @14:05:00.62 CC.seek :closed @14:05:04.88 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.13 @14:05:04.90 Hand.open @14:05:05.53 Forearm.reconfigure LooseForearm @14:05:05.58 Forearm.seek :retract @14:05:06.56 SC.coast @14:05:06.60 collectSample 1500ml w/bubble point of 55psi @14:05:06.62 Priming 7ml @14:05:06.63 Intake.select :close @14:05:06.64 Exhaust.select :close @14:05:06.66 SS.hold @14:05:06.90 Calibrating pressure and temperature sensors @14:05:06.92 CTV.dial :air @14:05:06.93 SSV.dial :spit,avoiding: :exhaust @14:05:06.96 SS.reconfigure SSfast @14:05:07.01 SS.seek 5ml @14:05:09.93 SSV.dial CBV @14:05:09.96
CBV.dial SSV @14:05:11.38 delay 3.8 seconds @14:05:15.32 SSV.dial :spit @14:05:16.70 SS.seek 0ml @14:05:19.42 SSV.dial CBV @14:05:20.81 CTV.dial :intake @14:05:21.60 SSV.dial CBV @14:05:21.63
CBV.dial SSV @14:05:21.65 Allowing 40s for 7.0ml of remaining 7.0ml @14:05:21.66 Intake.select :open @14:05:23.83 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:05:23.85 SS.reconfigure SSinhale.with maxSpeed: 150 @14:05:23.90 SS.seek 7ml @14:05:27.30 Vacuum too high -- waiting up to 40s @14:05:28.69 Intake @32.8psia, vacuum from 20.0 to 24.0psi @14:05:28.71 SS.reconfigure SSinhale.with maxSpeed: 135 @14:05:28.76 SS.seek 7ml @14:05:31.48 Vacuum too high -- waiting up to 40s @14:05:33.12 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:05:33.14 SS.reconfigure SSinhale.with maxSpeed: 122 @14:05:33.19 SS.seek 7ml @14:05:34.93 Stroke completed -- waiting for pressure to equalize ... @14:05:36.91 Intake.select :close @14:05:37.07 SSV.dial :exhaust @14:05:37.88 Exhaust.select :open @14:05:37.99 SS.reconfigure SSexhale @14:05:38.04 SS.seek 0ml @14:05:41.88 Exhaust.select :close @14:05:42.00 Sampling 1500.0ml @14:05:42.01 Intake.select :close @14:05:42.02 Exhaust.select :close @14:05:42.04 SS.reconfigure SSconfig @14:05:42.09 SS.hold @14:05:42.31 CTV.dial :intake @14:05:42.33 SSV.dial CBV @14:05:42.36
CBV.dial SSV @14:05:43.19 Allowing 130s for 25.0ml of remaining 1500.0ml @14:05:43.20 Intake.select :open @14:05:45.38 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:05:45.39 SS.reconfigure SSinhale.with maxSpeed: 122 @14:05:45.44 SS.seek 25ml @14:05:49.18 Vacuum too high -- waiting up to 40s @14:05:49.33 delay 1 second @14:05:50.34 AFV.select :holdingCoil @14:05:50.55 delay 1 second @14:05:50.59
Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:05:50.61 SS.reconfigure SSinhale.with maxSpeed: 110 @14:05:50.66 SS.seek 25ml @14:05:51.58 ATV.dial :waste @14:05:52.58 AS.reconfigure ASnorm @14:05:52.63 AS.home.seek :empty @14:05:53.46
Vacuum too high -- waiting up to 40s @14:05:54.84 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:05:54.85 SS.reconfigure SSinhale.with maxSpeed: 99 @14:05:54.90 SS.seek 25ml @14:05:57.48 Vacuum too high -- waiting up to 40s @14:05:58.83 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:05:58.84 SS.reconfigure SSinhale.with maxSpeed: 89 @14:05:58.89 SS.seek 25ml @14:06:01.64 Vacuum too high -- waiting up to 40s @14:06:03.55 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:06:03.57 SS.reconfigure SSinhale.with maxSpeed: 80 @14:06:03.62 SS.seek 25ml @14:06:07.27 Vacuum too high -- waiting up to 40s @14:06:08.35 Intake @32.7psia, vacuum from 20.0 to 24.0psi @14:06:08.37 SS.reconfigure SSinhale.with maxSpeed: 72 @14:06:08.42 SS.seek 25ml @14:06:12.57 Vacuum too high -- waiting up to 40s @14:06:13.95 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:06:13.96 SS.reconfigure SSinhale.with maxSpeed: 65 @14:06:14.01 SS.seek 25ml @14:06:19.39 Vacuum too high -- waiting up to 40s @14:06:20.74 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:06:20.76 SS.reconfigure SSinhale.with maxSpeed: 59 @14:06:20.81 SS.seek 25ml @14:06:24.07 Stroke completed -- waiting for pressure to equalize ... @14:06:26.70 Intake.select :close @14:06:26.86 SSV.dial :exhaust @14:06:27.66 Exhaust.select :open @14:06:27.77 SS.reconfigure SSexhale @14:06:27.82 SS.seek 0ml @14:06:36.92 Exhaust.select :close @14:06:37.04 SSV.dial CBV @14:06:37.07
CBV.dial SSV @14:06:37.91 Allowing 130s for 25.0ml of remaining 1475.0ml @14:06:37.92 Intake.select :open @14:06:40.10 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:06:40.12 SS.reconfigure SSinhale.with maxSpeed: 59 @14:06:40.17 SS.seek 25ml @14:06:45.21 ATV.dial :waste @14:06:45.23 ARV.dial between carrier and air1 @14:06:45.32 ASV.dial :waste1 @14:06:47.64
Vacuum too high -- waiting up to 40s @14:06:48.47 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:06:48.49 SS.reconfigure SSinhale.with maxSpeed: 53 @14:06:48.54 SS.seek 25ml @14:07:05.98 Vacuum too high -- waiting up to 40s @14:07:06.81 Intake @32.5psia, vacuum from 20.0 to 24.0psi @14:07:06.83 SS.reconfigure SSinhale.with maxSpeed: 48 @14:07:06.88 SS.seek 25ml @14:07:16.40 Stroke completed -- waiting for pressure to equalize ... @14:07:19.00 Intake.select :close @14:07:19.16 SSV.dial :exhaust @14:07:19.97 Exhaust.select :open @14:07:20.08 SS.reconfigure SSexhale @14:07:20.13 SS.seek 0ml @14:07:29.40 Exhaust.select :close @14:07:29.52 SSV.dial CBV @14:07:29.57
CBV.dial SSV @14:07:30.38 Allowing 130s for 25.0ml of remaining 1450.0ml @14:07:30.39 Intake.select :open @14:07:32.56 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:07:32.58 SS.reconfigure SSinhale.with maxSpeed: 48 @14:07:32.63 SS.seek 25ml @14:08:10.27 Stroke completed -- waiting for pressure to equalize ... @14:08:12.87 Intake.select :close @14:08:13.03 SSV.dial :exhaust @14:08:13.83 Exhaust.select :open @14:08:13.94 SS.reconfigure SSexhale @14:08:13.99 SS.seek 0ml @14:08:22.92 Exhaust.select :close @14:08:23.04 SSV.dial CBV @14:08:23.07
CBV.dial SSV @14:08:23.89 Allowing 130s for 25.0ml of remaining 1425.0ml @14:08:23.90 Intake.select :open @14:08:26.08 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:08:26.09 SS.reconfigure SSinhale.with maxSpeed: 48 @14:08:26.14 SS.seek 25ml @14:09:03.49 Stroke completed -- waiting for pressure to equalize ... @14:09:06.09 Intake.select :close @14:09:06.25 SSV.dial :exhaust @14:09:07.03 Exhaust.select :open @14:09:07.14 SS.reconfigure SSexhale @14:09:07.19 SS.seek 0ml @14:09:16.27 Exhaust.select :close @14:09:16.39 SSV.dial CBV @14:09:16.42
CBV.dial SSV @14:09:17.24 Allowing 130s for 25.0ml of remaining 1400.0ml @14:09:17.25 Intake.select :open @14:09:19.43 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:09:19.44 SS.reconfigure SSinhale.with maxSpeed: 48 @14:09:19.49 SS.seek 25ml @14:09:56.87 Stroke completed -- waiting for pressure to equalize ... @14:09:59.47 Intake.select :close @14:09:59.63 SSV.dial :exhaust @14:10:00.42 Exhaust.select :open @14:10:00.54 SS.reconfigure SSexhale @14:10:00.59 SS.seek 0ml @14:10:09.43 Exhaust.select :close @14:10:09.55 SSV.dial CBV @14:10:09.58
CBV.dial SSV @14:10:10.39 Allowing 130s for 25.0ml of remaining 1375.0ml @14:10:10.40 Intake.select :open @14:10:12.57 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:10:12.59 SS.reconfigure SSinhale.with maxSpeed: 48 @14:10:12.64 SS.seek 25ml @14:10:50.23 Stroke completed -- waiting for pressure to equalize ... @14:10:52.81 Intake.select :close @14:10:52.97 SSV.dial :exhaust @14:10:53.78 Exhaust.select :open @14:10:53.89 SS.reconfigure SSexhale @14:10:53.95 SS.seek 0ml @14:11:02.74 Exhaust.select :close @14:11:02.86 SSV.dial CBV @14:11:02.89
CBV.dial SSV @14:11:03.71 Allowing 130s for 25.0ml of remaining 1350.0ml @14:11:03.72 Intake.select :open @14:11:05.90 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:11:05.91 SS.reconfigure SSinhale.with maxSpeed: 48 @14:11:05.96 SS.seek 25ml @14:11:43.68 Stroke completed -- waiting for pressure to equalize ... @14:11:46.20 Intake.select :close @14:11:46.36 SSV.dial :exhaust @14:11:47.18 Exhaust.select :open @14:11:47.29 SS.reconfigure SSexhale @14:11:47.34 SS.seek 0ml @14:11:56.56 Exhaust.select :close @14:11:56.68 SSV.dial CBV @14:11:56.71
CBV.dial SSV @14:11:57.55 Allowing 130s for 25.0ml of remaining 1325.0ml @14:11:57.56 Intake.select :open @14:11:59.73 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:11:59.75 SS.reconfigure SSinhale.with maxSpeed: 48 @14:11:59.80 SS.seek 25ml @14:12:36.81 Stroke completed -- waiting for pressure to equalize ... @14:12:39.40 Intake.select :close @14:12:39.56 SSV.dial :exhaust @14:12:40.36 Exhaust.select :open @14:12:40.47 SS.reconfigure SSexhale @14:12:40.52 SS.seek 0ml @14:12:49.51 Exhaust.select :close @14:12:49.67 SSV.dial CBV @14:12:49.70
CBV.dial SSV @14:12:50.52 Allowing 130s for 25.0ml of remaining 1300.0ml @14:12:50.53 Intake.select :open @14:12:52.71 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:12:52.72 SS.reconfigure SSinhale.with maxSpeed: 48 @14:12:52.77 SS.seek 25ml @14:13:30.13 Stroke completed -- waiting for pressure to equalize ... @14:13:32.72 Intake.select :close @14:13:32.88 SSV.dial :exhaust @14:13:33.68 Exhaust.select :open @14:13:33.79 SS.reconfigure SSexhale @14:13:33.84 SS.seek 0ml @14:13:42.99 Exhaust.select :close @14:13:43.11 SSV.dial CBV @14:13:43.14
CBV.dial SSV @14:13:43.96 Allowing 130s for 25.0ml of remaining 1275.0ml @14:13:43.97 Intake.select :open @14:13:46.14 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:13:46.16 SS.reconfigure SSinhale.with maxSpeed: 48 @14:13:46.21 SS.seek 25ml @14:14:23.27 Stroke completed -- waiting for pressure to equalize ... @14:14:25.86 Intake.select :close @14:14:26.02 SSV.dial :exhaust @14:14:26.82 Exhaust.select :open @14:14:26.94 SS.reconfigure SSexhale @14:14:26.99 SS.seek 0ml @14:14:36.13 Exhaust.select :close @14:14:36.25 SSV.dial CBV @14:14:36.28
CBV.dial SSV @14:14:37.10 Allowing 130s for 25.0ml of remaining 1250.0ml @14:14:37.11 Intake.select :open @14:14:39.28 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:14:39.30 SS.reconfigure SSinhale.with maxSpeed: 48 @14:14:39.35 SS.seek 25ml @14:15:16.52 Stroke completed -- waiting for pressure to equalize ... @14:15:19.10 Intake.select :close @14:15:19.25 SSV.dial :exhaust @14:15:20.06 Exhaust.select :open @14:15:20.18 SS.reconfigure SSexhale @14:15:20.23 SS.seek 0ml @14:15:29.82 Exhaust.select :close @14:15:29.94 SSV.dial CBV @14:15:30.00
CBV.dial SSV @14:15:30.77 Allowing 130s for 25.0ml of remaining 1225.0ml @14:15:30.78 Intake.select :open @14:15:32.97 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:15:32.98 SS.reconfigure SSinhale.with maxSpeed: 48 @14:15:33.04 SS.seek 25ml @14:16:10.10 Stroke completed -- waiting for pressure to equalize ... @14:16:12.71 Intake.select :close @14:16:12.87 SSV.dial :exhaust @14:16:13.66 Exhaust.select :open @14:16:13.77 SS.reconfigure SSexhale @14:16:13.82 SS.seek 0ml @14:16:22.85 Exhaust.select :close @14:16:22.97 SSV.dial CBV @14:16:23.00
CBV.dial SSV @14:16:23.82 Allowing 130s for 25.0ml of remaining 1200.0ml @14:16:23.83 Intake.select :open @14:16:26.01 Intake @34.4psia, vacuum from 20.0 to 24.0psi @14:16:26.03 SS.reconfigure SSinhale.with maxSpeed: 48 @14:16:26.08 SS.seek 25ml @14:16:46.68 Vacuum too high -- waiting up to 40s @14:16:47.52 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:16:47.54 SS.reconfigure SSinhale.with maxSpeed: 43 @14:16:47.59 SS.seek 25ml @14:17:06.52 Stroke completed -- waiting for pressure to equalize ... @14:17:09.12 Intake.select :close @14:17:09.28 SSV.dial :exhaust @14:17:10.07 Exhaust.select :open @14:17:10.19 SS.reconfigure SSexhale @14:17:10.24 SS.seek 0ml @14:17:18.96 Exhaust.select :close @14:17:19.08 SSV.dial CBV @14:17:19.11
CBV.dial SSV @14:17:19.93 Allowing 130s for 25.0ml of remaining 1175.0ml @14:17:19.94 Intake.select :open @14:17:22.12 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:17:22.13 SS.reconfigure SSinhale.with maxSpeed: 43 @14:17:22.18 SS.seek 25ml @14:18:03.74 Stroke completed -- waiting for pressure to equalize ... @14:18:06.33 Intake.select :close @14:18:06.49 SSV.dial :exhaust @14:18:07.27 Exhaust.select :open @14:18:07.39 SS.reconfigure SSexhale @14:18:07.44 SS.seek 0ml @14:18:16.45 Exhaust.select :close @14:18:16.57 SSV.dial CBV @14:18:16.60
CBV.dial SSV @14:18:17.43 Allowing 130s for 25.0ml of remaining 1150.0ml @14:18:17.44 Intake.select :open @14:18:19.61 Intake @34.4psia, vacuum from 20.0 to 24.0psi @14:18:19.63 SS.reconfigure SSinhale.with maxSpeed: 43 @14:18:19.69 SS.seek 25ml @14:19:00.93 Stroke completed -- waiting for pressure to equalize ... @14:19:03.52 Intake.select :close @14:19:03.68 SSV.dial :exhaust @14:19:04.48 Exhaust.select :open @14:19:04.60 SS.reconfigure SSexhale @14:19:04.66 SS.seek 0ml @14:19:13.72 Exhaust.select :close @14:19:13.84 SSV.dial CBV @14:19:13.87
CBV.dial SSV @14:19:14.71 Allowing 130s for 25.0ml of remaining 1125.0ml @14:19:14.72 Intake.select :open @14:19:16.90 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:19:16.91 SS.reconfigure SSinhale.with maxSpeed: 43 @14:19:16.96 SS.seek 25ml @14:19:58.67 Stroke completed -- waiting for pressure to equalize ... @14:20:00.93 Intake.select :close @14:20:01.09 SSV.dial :exhaust @14:20:01.89 Exhaust.select :open @14:20:02.00 SS.reconfigure SSexhale @14:20:02.05 SS.seek 0ml @14:20:11.15 Exhaust.select :close @14:20:11.27 SSV.dial CBV @14:20:11.30
CBV.dial SSV @14:20:12.11 Allowing 130s for 25.0ml of remaining 1100.0ml @14:20:12.12 Intake.select :open @14:20:14.29 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:20:14.32 SS.reconfigure SSinhale.with maxSpeed: 43 @14:20:14.57 SS.seek 25ml @14:20:55.81 Stroke completed -- waiting for pressure to equalize ... @14:20:58.41 Intake.select :close @14:20:58.57 SSV.dial :exhaust @14:20:59.36 Exhaust.select :open @14:20:59.47 SS.reconfigure SSexhale @14:20:59.52 SS.seek 0ml @14:21:08.68 Exhaust.select :close @14:21:08.80 SSV.dial CBV @14:21:08.83
CBV.dial SSV @14:21:09.64 Allowing 130s for 25.0ml of remaining 1075.0ml @14:21:09.65 Intake.select :open @14:21:11.82 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:21:11.84 SS.reconfigure SSinhale.with maxSpeed: 43 @14:21:11.89 SS.seek 25ml @14:21:53.43 Stroke completed -- waiting for pressure to equalize ... @14:21:56.03 Intake.select :close @14:21:56.18 SSV.dial :exhaust @14:21:56.99 Exhaust.select :open @14:21:57.10 SS.reconfigure SSexhale @14:21:57.15 SS.seek 0ml @14:22:06.29 Exhaust.select :close @14:22:06.41 SSV.dial CBV @14:22:06.44
CBV.dial SSV @14:22:07.25 Allowing 130s for 25.0ml of remaining 1050.0ml @14:22:07.26 Intake.select :open @14:22:09.43 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:22:09.45 SS.reconfigure SSinhale.with maxSpeed: 43 @14:22:09.50 SS.seek 25ml @14:22:50.79 Stroke completed -- waiting for pressure to equalize ... @14:22:53.39 Intake.select :close @14:22:53.55 SSV.dial :exhaust @14:22:54.35 Exhaust.select :open @14:22:54.46 SS.reconfigure SSexhale @14:22:54.51 SS.seek 0ml @14:23:03.24 Exhaust.select :close @14:23:03.36 SSV.dial CBV @14:23:03.39
CBV.dial SSV @14:23:04.21 Allowing 130s for 25.0ml of remaining 1025.0ml @14:23:04.22 Intake.select :open @14:23:06.39 Intake @34.8psia, vacuum from 20.0 to 24.0psi @14:23:06.41 SS.reconfigure SSinhale.with maxSpeed: 43 @14:23:06.46 SS.seek 25ml @14:23:48.18 Stroke completed -- waiting for pressure to equalize ... @14:23:50.69 Intake.select :close @14:23:50.85 SSV.dial :exhaust @14:23:51.64 Exhaust.select :open @14:23:51.75 SS.reconfigure SSexhale @14:23:51.80 SS.seek 0ml @14:24:00.88 Exhaust.select :close @14:24:01.00 SSV.dial CBV @14:24:01.04
CBV.dial SSV @14:24:01.86 Allowing 130s for 25.0ml of remaining 1000.0ml @14:24:01.87 Intake.select :open @14:24:04.04 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:24:04.06 SS.reconfigure SSinhale.with maxSpeed: 43 @14:24:04.11 SS.seek 25ml @14:24:45.40 Stroke completed -- waiting for pressure to equalize ... @14:24:48.00 Intake.select :close @14:24:48.16 SSV.dial :exhaust @14:24:48.97 Exhaust.select :open @14:24:49.08 SS.reconfigure SSexhale @14:24:49.13 SS.seek 0ml @14:24:58.45 Exhaust.select :close @14:24:58.57 SSV.dial CBV @14:24:58.60
CBV.dial SSV @14:24:59.43 Allowing 130s for 25.0ml of remaining 975.0ml @14:24:59.44 Intake.select :open @14:25:01.62 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:25:01.63 SS.reconfigure SSinhale.with maxSpeed: 43 @14:25:01.68 SS.seek 25ml @14:25:43.10 Stroke completed -- waiting for pressure to equalize ... @14:25:45.69 Intake.select :close @14:25:45.85 SSV.dial :exhaust @14:25:46.66 Exhaust.select :open @14:25:46.77 SS.reconfigure SSexhale @14:25:46.82 SS.seek 0ml @14:25:55.52 Exhaust.select :close @14:25:55.64 SSV.dial CBV @14:25:55.67
CBV.dial SSV @14:25:56.50 Allowing 130s for 25.0ml of remaining 950.0ml @14:25:56.51 Intake.select :open @14:25:58.68 Intake @34.7psia, vacuum from 20.0 to 24.0psi @14:25:58.70 SS.reconfigure SSinhale.with maxSpeed: 43 @14:25:58.75 SS.seek 25ml @14:26:39.23 Vacuum too high -- waiting up to 40s @14:26:40.05 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:26:40.07 SS.reconfigure SSinhale.with maxSpeed: 39 @14:26:40.12 SS.seek 25ml @14:26:41.65 Stroke completed -- waiting for pressure to equalize ... @14:26:43.62 Intake.select :close @14:26:43.78 SSV.dial :exhaust @14:26:44.59 Exhaust.select :open @14:26:44.70 SS.reconfigure SSexhale @14:26:44.76 SS.seek 0ml @14:26:53.70 Exhaust.select :close @14:26:53.82 SSV.dial CBV @14:26:53.85
CBV.dial SSV @14:26:54.69 Allowing 130s for 25.0ml of remaining 925.0ml @14:26:54.70 Intake.select :open @14:26:56.87 Intake @35.0psia, vacuum from 20.0 to 24.0psi @14:26:56.89 SS.reconfigure SSinhale.with maxSpeed: 39 @14:26:56.94 SS.seek 25ml @14:27:42.73 Stroke completed -- waiting for pressure to equalize ... @14:27:45.85 Intake.select :close @14:27:46.01 SSV.dial :exhaust @14:27:46.82 Exhaust.select :open @14:27:46.93 SS.reconfigure SSexhale @14:27:46.98 SS.seek 0ml @14:27:56.06 Exhaust.select :close @14:27:56.18 SSV.dial CBV @14:27:56.21
CBV.dial SSV @14:27:57.03 Allowing 130s for 25.0ml of remaining 900.0ml @14:27:57.04 Intake.select :open @14:27:59.22 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:27:59.23 SS.reconfigure SSinhale.with maxSpeed: 39 @14:27:59.28 SS.seek 25ml @14:28:44.56 Stroke completed -- waiting for pressure to equalize ... @14:28:47.17 Intake.select :close @14:28:47.33 SSV.dial :exhaust @14:28:48.14 Exhaust.select :open @14:28:48.25 SS.reconfigure SSexhale @14:28:48.30 SS.seek 0ml @14:28:57.38 Exhaust.select :close @14:28:57.50 SSV.dial CBV @14:28:57.53
CBV.dial SSV @14:28:58.35 Allowing 130s for 25.0ml of remaining 875.0ml @14:28:58.36 Intake.select :open @14:29:00.53 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:29:00.55 SS.reconfigure SSinhale.with maxSpeed: 39 @14:29:00.60 SS.seek 25ml @14:29:46.23 Stroke completed -- waiting for pressure to equalize ... @14:29:48.83 Intake.select :close @14:29:49.03 SSV.dial :exhaust @14:29:49.85 Exhaust.select :open @14:29:49.96 SS.reconfigure SSexhale @14:29:50.01 SS.seek 0ml @14:29:59.59 Exhaust.select :close @14:29:59.71 SSV.dial CBV @14:29:59.74
CBV.dial SSV @14:30:00.57 Allowing 130s for 25.0ml of remaining 850.0ml @14:30:00.58 Intake.select :open @14:30:02.75 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:30:02.77 SS.reconfigure SSinhale.with maxSpeed: 39 @14:30:02.82 SS.seek 25ml @14:30:48.37 Stroke completed -- waiting for pressure to equalize ... @14:30:50.99 Intake.select :close @14:30:51.15 SSV.dial :exhaust @14:30:51.96 Exhaust.select :open @14:30:52.07 SS.reconfigure SSexhale @14:30:52.12 SS.seek 0ml @14:31:01.10 Exhaust.select :close @14:31:01.22 SSV.dial CBV @14:31:01.25
CBV.dial SSV @14:31:02.08 Allowing 130s for 25.0ml of remaining 825.0ml @14:31:02.09 Intake.select :open @14:31:04.26 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:31:04.28 SS.reconfigure SSinhale.with maxSpeed: 39 @14:31:04.33 SS.seek 25ml @14:31:50.06 Stroke completed -- waiting for pressure to equalize ... @14:31:52.64 Intake.select :close @14:31:52.80 SSV.dial :exhaust @14:31:53.61 Exhaust.select :open @14:31:53.72 SS.reconfigure SSexhale @14:31:53.78 SS.seek 0ml @14:32:02.99 Exhaust.select :close @14:32:03.11 SSV.dial CBV @14:32:03.14
CBV.dial SSV @14:32:04.50 Allowing 130s for 25.0ml of remaining 800.0ml @14:32:04.51 Intake.select :open @14:32:06.69 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:32:06.70 SS.reconfigure SSinhale.with maxSpeed: 39 @14:32:06.75 SS.seek 25ml @14:32:51.92 Stroke completed -- waiting for pressure to equalize ... @14:32:54.52 Intake.select :close @14:32:54.68 SSV.dial :exhaust @14:32:55.49 Exhaust.select :open @14:32:55.60 SS.reconfigure SSexhale @14:32:55.65 SS.seek 0ml @14:33:04.81 Exhaust.select :close @14:33:04.93 SSV.dial CBV @14:33:04.96
CBV.dial SSV @14:33:05.80 Allowing 130s for 25.0ml of remaining 775.0ml @14:33:05.81 Intake.select :open @14:33:07.98 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:33:08.00 SS.reconfigure SSinhale.with maxSpeed: 39 @14:33:08.05 SS.seek 25ml @14:33:53.40 Stroke completed -- waiting for pressure to equalize ... @14:33:56.64 Intake.select :close @14:33:56.80 SSV.dial :exhaust @14:33:57.61 Exhaust.select :open @14:33:57.72 SS.reconfigure SSexhale @14:33:57.77 SS.seek 0ml @14:34:07.31 Exhaust.select :close @14:34:07.43 SSV.dial CBV @14:34:07.46
CBV.dial SSV @14:34:08.28 Allowing 130s for 25.0ml of remaining 750.0ml @14:34:08.29 Intake.select :open @14:34:10.46 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:34:10.48 SS.reconfigure SSinhale.with maxSpeed: 39 @14:34:10.53 SS.seek 25ml @14:34:55.93 Stroke completed -- waiting for pressure to equalize ... @14:34:59.13 Intake.select :close @14:34:59.29 SSV.dial :exhaust @14:35:00.07 Exhaust.select :open @14:35:00.19 SS.reconfigure SSexhale @14:35:00.25 SS.seek 0ml @14:35:09.29 Exhaust.select :close @14:35:09.42 SSV.dial CBV @14:35:09.48
CBV.dial SSV @14:35:10.33 Allowing 130s for 25.0ml of remaining 725.0ml @14:35:10.34 Intake.select :open @14:35:12.51 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:35:12.53 SS.reconfigure SSinhale.with maxSpeed: 39 @14:35:12.58 SS.seek 25ml @14:35:57.90 Stroke completed -- waiting for pressure to equalize ... @14:36:01.09 Intake.select :close @14:36:01.24 SSV.dial :exhaust @14:36:02.04 Exhaust.select :open @14:36:02.16 SS.reconfigure SSexhale @14:36:02.21 SS.seek 0ml @14:36:11.45 Exhaust.select :close @14:36:11.57 SSV.dial CBV @14:36:11.60
CBV.dial SSV @14:36:12.42 Allowing 130s for 25.0ml of remaining 700.0ml @14:36:12.43 Intake.select :open @14:36:14.60 Intake @34.7psia, vacuum from 20.0 to 24.0psi @14:36:14.62 SS.reconfigure SSinhale.with maxSpeed: 39 @14:36:14.67 SS.seek 25ml @14:36:41.60 Vacuum too high -- waiting up to 40s @14:36:41.93 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:36:41.95 SS.reconfigure SSinhale.with maxSpeed: 35 @14:36:42.00 SS.seek 25ml @14:37:02.96 Stroke completed -- waiting for pressure to equalize ... @14:37:05.55 Intake.select :close @14:37:05.71 SSV.dial :exhaust @14:37:06.50 Exhaust.select :open @14:37:06.61 SS.reconfigure SSexhale @14:37:06.66 SS.seek 0ml @14:37:15.54 Exhaust.select :close @14:37:15.66 SSV.dial CBV @14:37:15.69
CBV.dial SSV @14:37:16.50 Allowing 130s for 25.0ml of remaining 675.0ml @14:37:16.51 Intake.select :open @14:37:18.68 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:37:18.70 SS.reconfigure SSinhale.with maxSpeed: 35 @14:37:18.75 SS.seek 25ml @14:38:09.65 Stroke completed -- waiting for pressure to equalize ... @14:38:12.25 Intake.select :close @14:38:12.41 SSV.dial :exhaust @14:38:13.22 Exhaust.select :open @14:38:13.33 SS.reconfigure SSexhale @14:38:13.38 SS.seek 0ml @14:38:22.51 Exhaust.select :close @14:38:22.63 SSV.dial CBV @14:38:22.66
CBV.dial SSV @14:38:23.49 Allowing 130s for 25.0ml of remaining 650.0ml @14:38:23.50 Intake.select :open @14:38:25.67 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:38:25.69 SS.reconfigure SSinhale.with maxSpeed: 35 @14:38:25.74 SS.seek 25ml @14:39:16.06 Stroke completed -- waiting for pressure to equalize ... @14:39:19.28 Intake.select :close @14:39:19.44 SSV.dial :exhaust @14:39:20.22 Exhaust.select :open @14:39:20.33 SS.reconfigure SSexhale @14:39:20.38 SS.seek 0ml @14:39:29.60 Exhaust.select :close @14:39:29.72 SSV.dial CBV @14:39:29.75
CBV.dial SSV @14:39:30.57 Allowing 130s for 25.0ml of remaining 625.0ml @14:39:30.58 Intake.select :open @14:39:32.75 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:39:32.77 SS.reconfigure SSinhale.with maxSpeed: 35 @14:39:32.83 SS.seek 25ml @14:40:22.79 Stroke completed -- waiting for pressure to equalize ... @14:40:26.00 Intake.select :close @14:40:26.16 SSV.dial :exhaust @14:40:26.96 Exhaust.select :open @14:40:27.08 SS.reconfigure SSexhale @14:40:27.13 SS.seek 0ml @14:40:36.57 Exhaust.select :close @14:40:36.69 SSV.dial CBV @14:40:36.72
CBV.dial SSV @14:40:37.53 Allowing 130s for 25.0ml of remaining 600.0ml @14:40:37.54 Intake.select :open @14:40:39.71 Intake @34.5psia, vacuum from 20.0 to 24.0psi @14:40:39.73 SS.reconfigure SSinhale.with maxSpeed: 35 @14:40:39.77 SS.seek 25ml @14:41:30.32 Stroke completed -- waiting for pressure to equalize ... @14:41:33.47 Intake.select :close @14:41:33.63 SSV.dial :exhaust @14:41:34.44 Exhaust.select :open @14:41:34.55 SS.reconfigure SSexhale @14:41:34.60 SS.seek 0ml @14:41:43.65 Exhaust.select :close @14:41:43.77 SSV.dial CBV @14:41:43.80
CBV.dial SSV @14:41:44.63 Allowing 130s for 25.0ml of remaining 575.0ml @14:41:44.64 Intake.select :open @14:41:46.82 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:41:46.83 SS.reconfigure SSinhale.with maxSpeed: 35 @14:41:46.88 SS.seek 25ml @14:42:37.26 Stroke completed -- waiting for pressure to equalize ... @14:42:40.50 Intake.select :close @14:42:40.66 SSV.dial :exhaust @14:42:41.47 Exhaust.select :open @14:42:41.58 SS.reconfigure SSexhale @14:42:41.63 SS.seek 0ml @14:42:50.90 Exhaust.select :close @14:42:51.02 SSV.dial CBV @14:42:51.05
CBV.dial SSV @14:42:51.88 Allowing 130s for 25.0ml of remaining 550.0ml @14:42:51.89 Intake.select :open @14:42:54.06 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:42:54.08 SS.reconfigure SSinhale.with maxSpeed: 35 @14:42:54.13 SS.seek 25ml @14:43:44.54 Stroke completed -- waiting for pressure to equalize ... @14:43:48.40 Intake.select :close @14:43:48.56 SSV.dial :exhaust @14:43:49.34 Exhaust.select :open @14:43:49.46 SS.reconfigure SSexhale @14:43:49.52 SS.seek 0ml @14:43:58.60 Exhaust.select :close @14:43:58.72 SSV.dial CBV @14:43:58.75
CBV.dial SSV @14:43:59.57 Allowing 130s for 25.0ml of remaining 525.0ml @14:43:59.58 Intake.select :open @14:44:01.76 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:44:01.77 SS.reconfigure SSinhale.with maxSpeed: 35 @14:44:01.82 SS.seek 25ml @14:44:41.96 Vacuum too high -- waiting up to 40s @14:44:42.78 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:44:42.80 SS.reconfigure SSinhale.with maxSpeed: 32 @14:44:42.85 SS.seek 25ml @14:44:54.20 Stroke completed -- waiting for pressure to equalize ... @14:44:57.42 Intake.select :close @14:44:57.57 SSV.dial :exhaust @14:44:58.38 Exhaust.select :open @14:44:58.49 SS.reconfigure SSexhale @14:44:58.54 SS.seek 0ml @14:45:07.75 Exhaust.select :close @14:45:07.86 SSV.dial CBV @14:45:07.89
CBV.dial SSV @14:45:09.25 Allowing 130s for 25.0ml of remaining 500.0ml @14:45:09.26 Intake.select :open @14:45:11.43 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:45:11.45 SS.reconfigure SSinhale.with maxSpeed: 32 @14:45:11.50 SS.seek 25ml @14:46:06.60 Stroke completed -- waiting for pressure to equalize ... @14:46:09.85 Intake.select :close @14:46:10.01 SSV.dial :exhaust @14:46:10.80 Exhaust.select :open @14:46:10.91 SS.reconfigure SSexhale @14:46:10.96 SS.seek 0ml @14:46:20.15 Exhaust.select :close @14:46:20.27 SSV.dial CBV @14:46:20.30
CBV.dial SSV @14:46:21.13 Allowing 130s for 25.0ml of remaining 475.0ml @14:46:21.14 Intake.select :open @14:46:23.32 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:46:23.34 SS.reconfigure SSinhale.with maxSpeed: 32 @14:46:23.39 SS.seek 25ml @14:47:18.45 Stroke completed -- waiting for pressure to equalize ... @14:47:22.33 Intake.select :close @14:47:22.49 SSV.dial :exhaust @14:47:23.29 Exhaust.select :open @14:47:23.41 SS.reconfigure SSexhale @14:47:23.47 SS.seek 0ml @14:47:32.32 Exhaust.select :close @14:47:32.44 SSV.dial CBV @14:47:32.47
CBV.dial SSV @14:47:33.30 Allowing 130s for 25.0ml of remaining 450.0ml @14:47:33.31 Intake.select :open @14:47:35.48 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:47:35.50 SS.reconfigure SSinhale.with maxSpeed: 32 @14:47:35.55 SS.seek 25ml @14:48:30.90 Stroke completed -- waiting for pressure to equalize ... @14:48:34.74 Intake.select :close @14:48:34.89 SSV.dial :exhaust @14:48:35.71 Exhaust.select :open @14:48:35.82 SS.reconfigure SSexhale @14:48:35.87 SS.seek 0ml @14:48:45.20 Exhaust.select :close @14:48:45.32 SSV.dial CBV @14:48:45.35
CBV.dial SSV @14:48:46.17 Allowing 130s for 25.0ml of remaining 425.0ml @14:48:46.18 Intake.select :open @14:48:48.35 Intake @32.4psia, vacuum from 20.0 to 24.0psi @14:48:48.37 SS.reconfigure SSinhale.with maxSpeed: 32 @14:48:48.42 SS.seek 25ml @14:49:43.16 Stroke completed -- waiting for pressure to equalize ... @14:49:46.99 Intake.select :close @14:49:47.15 SSV.dial :exhaust @14:49:47.94 Exhaust.select :open @14:49:48.05 SS.reconfigure SSexhale @14:49:48.10 SS.seek 0ml @14:49:57.32 Exhaust.select :close @14:49:57.99 SSV.dial CBV @14:49:58.02
CBV.dial SSV @14:49:58.83 Allowing 130s for 25.0ml of remaining 400.0ml @14:49:58.84 Intake.select :open @14:50:01.01 Intake @34.3psia, vacuum from 20.0 to 24.0psi @14:50:01.03 SS.reconfigure SSinhale.with maxSpeed: 32 @14:50:01.08 SS.seek 25ml @14:50:28.46 Vacuum too high -- waiting up to 40s @14:50:29.30 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:50:29.32 SS.reconfigure SSinhale.with maxSpeed: 29 @14:50:29.37 SS.seek 25ml @14:50:59.91 Stroke completed -- waiting for pressure to equalize ... @14:51:03.77 Intake.select :close @14:51:03.93 SSV.dial :exhaust @14:51:04.74 Exhaust.select :open @14:51:04.85 SS.reconfigure SSexhale @14:51:04.90 SS.seek 0ml @14:51:14.23 Exhaust.select :close @14:51:14.35 SSV.dial CBV @14:51:14.38
CBV.dial SSV @14:51:15.21 Allowing 130s for 25.0ml of remaining 375.0ml @14:51:15.22 Intake.select :open @14:51:17.40 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:51:17.41 SS.reconfigure SSinhale.with maxSpeed: 29 @14:51:17.46 SS.seek 25ml @14:52:17.85 Stroke completed -- waiting for pressure to equalize ... @14:52:21.70 Intake.select :close @14:52:21.86 SSV.dial :exhaust @14:52:22.66 Exhaust.select :open @14:52:22.77 SS.reconfigure SSexhale @14:52:22.82 SS.seek 0ml @14:52:31.71 Exhaust.select :close @14:52:31.83 SSV.dial CBV @14:52:31.86
CBV.dial SSV @14:52:32.69 Allowing 130s for 25.0ml of remaining 350.0ml @14:52:32.70 Intake.select :open @14:52:34.87 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:52:34.89 SS.reconfigure SSinhale.with maxSpeed: 29 @14:52:34.94 SS.seek 25ml @14:53:35.85 Stroke completed -- waiting for pressure to equalize ... @14:53:39.69 Intake.select :close @14:53:39.85 SSV.dial :exhaust @14:53:40.66 Exhaust.select :open @14:53:40.77 SS.reconfigure SSexhale @14:53:40.82 SS.seek 0ml @14:53:49.82 Exhaust.select :close @14:53:49.94 SSV.dial CBV @14:53:49.97
CBV.dial SSV @14:53:50.80 Allowing 130s for 25.0ml of remaining 325.0ml @14:53:50.81 Intake.select :open @14:53:52.99 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:53:53.00 SS.reconfigure SSinhale.with maxSpeed: 29 @14:53:53.05 SS.seek 25ml @14:54:53.98 Stroke completed -- waiting for pressure to equalize ... @14:54:58.39 Intake.select :close @14:54:58.55 SSV.dial :exhaust @14:54:59.37 Exhaust.select :open @14:54:59.48 SS.reconfigure SSexhale @14:54:59.53 SS.seek 0ml @14:55:08.81 Exhaust.select :close @14:55:08.93 SSV.dial CBV @14:55:08.98
CBV.dial SSV @14:55:09.77 Allowing 130s for 25.0ml of remaining 300.0ml @14:55:09.78 Intake.select :open @14:55:11.95 Intake @34.6psia, vacuum from 20.0 to 24.0psi @14:55:11.97 SS.reconfigure SSinhale.with maxSpeed: 29 @14:55:12.02 SS.seek 25ml @14:55:32.89 Vacuum too high -- waiting up to 40s @14:55:33.73 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:55:33.75 SS.reconfigure SSinhale.with maxSpeed: 26 @14:55:33.81 SS.seek 25ml @14:56:18.57 Stroke completed -- waiting for pressure to equalize ... @14:56:22.44 Intake.select :close @14:56:22.60 SSV.dial :exhaust @14:56:23.41 Exhaust.select :open @14:56:23.52 SS.reconfigure SSexhale @14:56:23.57 SS.seek 0ml @14:56:32.68 Exhaust.select :close @14:56:32.80 SSV.dial CBV @14:56:32.83
CBV.dial SSV @14:56:33.67 Allowing 130s for 25.0ml of remaining 275.0ml @14:56:33.68 Intake.select :open @14:56:35.85 Intake @34.5psia, vacuum from 20.0 to 24.0psi @14:56:35.87 SS.reconfigure SSinhale.with maxSpeed: 26 @14:56:35.92 SS.seek 25ml @14:57:43.30 Stroke completed -- waiting for pressure to equalize ... @14:57:47.77 Intake.select :close @14:57:47.93 SSV.dial :exhaust @14:57:48.75 Exhaust.select :open @14:57:48.86 SS.reconfigure SSexhale @14:57:48.91 SS.seek 0ml @14:57:58.16 Exhaust.select :close @14:57:58.28 SSV.dial CBV @14:57:58.31
CBV.dial SSV @14:57:59.13 Allowing 130s for 25.0ml of remaining 250.0ml @14:57:59.14 Intake.select :open @14:58:01.31 Intake @34.5psia, vacuum from 20.0 to 24.0psi @14:58:01.33 SS.reconfigure SSinhale.with maxSpeed: 26 @14:58:01.38 SS.seek 25ml @14:59:08.87 Stroke completed -- waiting for pressure to equalize ... @14:59:13.32 Intake.select :close @14:59:13.48 SSV.dial :exhaust @14:59:14.83 Exhaust.select :open @14:59:14.94 SS.reconfigure SSexhale @14:59:14.99 SS.seek 0ml @14:59:23.90 Exhaust.select :close @14:59:24.02 SSV.dial CBV @14:59:24.05
CBV.dial SSV @14:59:24.89 Allowing 130s for 25.0ml of remaining 225.0ml @14:59:24.90 Intake.select :open @14:59:27.07 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:59:27.09 SS.reconfigure SSinhale.with maxSpeed: 26 @14:59:27.14 SS.seek 25ml @15:00:35.06 Stroke completed -- waiting for pressure to equalize ... @15:00:39.56 Intake.select :close @15:00:39.72 SSV.dial :exhaust @15:00:40.54 Exhaust.select :open @15:00:40.65 SS.reconfigure SSexhale @15:00:40.70 SS.seek 0ml @15:00:49.88 Exhaust.select :close @15:00:50.00 SSV.dial CBV @15:00:50.03
CBV.dial SSV @15:00:50.85 Allowing 130s for 25.0ml of remaining 200.0ml @15:00:50.86 Intake.select :open @15:00:53.03 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:00:53.05 SS.reconfigure SSinhale.with maxSpeed: 26 @15:00:53.10 SS.seek 25ml @15:01:36.71 Vacuum too high -- waiting up to 40s @15:01:37.53 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:01:37.55 SS.reconfigure SSinhale.with maxSpeed: 23 @15:01:37.60 SS.seek 25ml @15:02:04.59 Stroke completed -- waiting for pressure to equalize ... @15:02:09.07 Intake.select :close @15:02:09.23 SSV.dial :exhaust @15:02:10.02 Exhaust.select :open @15:02:10.14 SS.reconfigure SSexhale @15:02:10.19 SS.seek 0ml @15:02:19.51 Exhaust.select :close @15:02:19.63 SSV.dial CBV @15:02:19.66
CBV.dial SSV @15:02:20.49 Allowing 130s for 25.0ml of remaining 175.0ml @15:02:20.50 Intake.select :open @15:02:22.68 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:02:22.70 SS.reconfigure SSinhale.with maxSpeed: 23 @15:02:22.75 SS.seek 25ml @15:03:38.57 Stroke completed -- waiting for pressure to equalize ... @15:03:43.03 Intake.select :close @15:03:43.19 SSV.dial :exhaust @15:03:44.00 Exhaust.select :open @15:03:44.11 SS.reconfigure SSexhale @15:03:44.16 SS.seek 0ml @15:03:53.35 Exhaust.select :close @15:03:53.47 SSV.dial CBV @15:03:53.50
CBV.dial SSV @15:03:54.32 Allowing 130s for 25.0ml of remaining 150.0ml @15:03:54.33 Intake.select :open @15:03:57.06 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:03:57.07 SS.reconfigure SSinhale.with maxSpeed: 23 @15:03:57.12 SS.seek 25ml @15:05:13.07 Stroke completed -- waiting for pressure to equalize ... @15:05:18.20 Intake.select :close @15:05:18.36 SSV.dial :exhaust @15:05:19.16 Exhaust.select :open @15:05:19.27 SS.reconfigure SSexhale @15:05:19.32 SS.seek 0ml @15:05:28.38 Exhaust.select :close @15:05:28.50 SSV.dial CBV @15:05:28.53
CBV.dial SSV @15:05:29.36 Allowing 130s for 25.0ml of remaining 125.0ml @15:05:29.37 Intake.select :open @15:05:31.54 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:05:31.56 SS.reconfigure SSinhale.with maxSpeed: 23 @15:05:31.61 SS.seek 25ml @15:06:48.01 Stroke completed -- waiting for pressure to equalize ... @15:06:53.13 Intake.select :close @15:06:53.28 SSV.dial :exhaust @15:06:54.10 Exhaust.select :open @15:06:54.21 SS.reconfigure SSexhale @15:06:54.26 SS.seek 0ml @15:07:03.13 Exhaust.select :close @15:07:03.25 SSV.dial CBV @15:07:03.28
CBV.dial SSV @15:07:04.10 Allowing 130s for 25.0ml of remaining 100.0ml @15:07:04.11 Intake.select :open @15:07:06.28 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:07:06.30 SS.reconfigure SSinhale.with maxSpeed: 23 @15:07:06.35 SS.seek 25ml @15:08:23.01 Stroke completed -- waiting for pressure to equalize ... @15:08:28.76 Intake.select :close @15:08:28.92 SSV.dial :exhaust @15:08:29.71 Exhaust.select :open @15:08:29.83 SS.reconfigure SSexhale @15:08:29.88 SS.seek 0ml @15:08:39.31 Exhaust.select :close @15:08:39.43 SSV.dial CBV @15:08:39.46
CBV.dial SSV @15:08:40.30 Allowing 130s for 25.0ml of remaining 75.0ml @15:08:40.31 Intake.select :open @15:08:42.48 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:08:42.50 SS.reconfigure SSinhale.with maxSpeed: 23 @15:08:42.55 SS.seek 25ml @15:09:36.26 Vacuum too high -- waiting up to 40s @15:09:37.98 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:09:38.00 SS.reconfigure SSinhale.with maxSpeed: 21 @15:09:38.05 SS.seek 25ml @15:10:03.18 Stroke completed -- waiting for pressure to equalize ... @15:10:09.61 Intake.select :close @15:10:09.77 SSV.dial :exhaust @15:10:10.58 Exhaust.select :open @15:10:10.69 SS.reconfigure SSexhale @15:10:10.74 SS.seek 0ml @15:10:19.90 Exhaust.select :close @15:10:20.02 SSV.dial CBV @15:10:20.05
CBV.dial SSV @15:10:20.88 Allowing 130s for 25.0ml of remaining 50.0ml @15:10:20.89 Intake.select :open @15:10:23.06 Intake @32.9psia, vacuum from 20.0 to 24.0psi @15:10:23.08 SS.reconfigure SSinhale.with maxSpeed: 21 @15:10:23.13 SS.seek 25ml @15:11:46.43 Stroke completed -- waiting for pressure to equalize ... @15:11:52.80 Intake.select :close @15:11:52.96 SSV.dial :exhaust @15:11:53.77 Exhaust.select :open @15:11:53.88 SS.reconfigure SSexhale @15:11:53.93 SS.seek 0ml @15:12:03.25 Exhaust.select :close @15:12:03.39 SSV.dial CBV @15:12:03.42
CBV.dial SSV @15:12:04.25 Allowing 130s for 25.0ml of remaining 25.0ml @15:12:04.26 Intake.select :open @15:12:06.43 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:12:06.45 SS.reconfigure SSinhale.with maxSpeed: 21 @15:12:06.50 SS.seek 24.96ml @15:13:10.12 Vacuum too high -- waiting up to 40s @15:13:11.20 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:13:11.22 SS.reconfigure SSinhale.with maxSpeed: 19 @15:13:11.27 SS.seek 24.96ml @15:13:32.70 Stroke completed -- waiting for pressure to equalize ... @15:13:38.45 Intake.select :close @15:13:39.15 SSV.dial :exhaust @15:13:39.97 Exhaust.select :open @15:13:40.08 SS.reconfigure SSexhale @15:13:40.13 SS.seek 0ml @15:13:49.65 Exhaust.select :close @15:13:49.77 Sampled 1500.0ml @15:13:49.79 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @15:13:49.80 Intake.select :close @15:13:49.87 CSV.dial :waste @15:13:49.96 CS.seek 0ml @15:13:50.04 SSV.dial CBV,avoiding: :exhaust @15:13:50.07 CSV.dial :air @15:13:50.11
CBV.dial SSV @15:13:50.92 CS.seek 10ml @15:13:52.11
CTV.dial :air @15:13:52.91 delay 15 seconds @15:14:07.94 SSV.dial :spit,avoiding: :exhaust @15:14:09.31 CTV.dial CSV,avoiding: :intake @15:14:09.36
SS.seek 0ml @15:14:09.50 SSV.dial CBV,avoiding: :exhaust @15:14:15.53 CBV.dial between SSV and waste2 @15:14:15.95 CTV.dial CSV,avoiding: :air @15:14:15.98
CSV.dial CTV @15:14:16.47 CS.reconfigure CSpressurize @15:14:16.52 CS.seek 0ml @15:14:42.16 BubblePoint: Intake @68.7psia, Outlet @13.2psia @15:14:42.17 CBV.dial SSV @15:14:42.54 SS.reconfigure SSconfig @15:14:42.59 SS.seek 10ml @15:14:48.11 delay 15 seconds @15:15:03.26 Evacuated: Intake @34.2psia, Outlet @-0.3psia @15:15:03.27 SSV.dial :spit,avoiding: :exhaust @15:15:04.66 SS.reconfigure SSexhale @15:15:04.71 SS.seek 0ml @15:15:09.73 SS.reconfigure SSfast @15:15:09.78 SS.seek 5ml @15:15:12.77 SSV.dial CBV,avoiding: :exhaust @15:15:12.82
CBV.dial SSV @15:15:14.26 CTV.dial :air,avoiding: :intake @15:15:15.64 CSV.dial CTV @15:15:15.68
SS.seek 10ml @15:15:16.67 CSV.dial :air @15:15:17.36 CS.reconfigure CSfast @15:15:17.96 CS.seek 0ml @15:15:18.65
SSV.dial :spit,avoiding: :exhaust @15:15:19.75 CS.coast @15:15:20.46
SS.reconfigure SSexhale @15:15:21.17 SS.seek 0ml @15:15:26.88 SS.reconfigure SSfast @15:15:27.11 SS.seek 5ml @15:15:29.75 SSV.dial CBV,avoiding: :exhaust @15:15:29.78
CBV.dial SSV @15:15:32.20 SSV.dial :spit,avoiding: :exhaust @15:15:33.56 SS.reconfigure SSconfig @15:15:33.61 SS.seek 0ml @15:15:36.74 SS.coast @15:15:36.79 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @15:15:36.80 Intake.select :close @15:15:36.88 CSV.dial :waste @15:15:37.08 SSV.dial CBV,avoiding: :exhaust @15:15:37.13
CBV.dial SSV @15:15:37.72 CS.seek 0ml @15:15:37.79 CSV.dial :air @15:15:38.52
CTV.dial :air @15:15:38.53 delay 15 seconds @15:15:39.12 CS.seek 10ml @15:15:54.02
SSV.dial :spit,avoiding: :exhaust @15:15:55.39 CTV.dial CSV,avoiding: :intake @15:15:55.43
SS.reconfigure SSexhale @15:15:55.55 SS.seek 0ml @15:15:55.80 SSV.dial CBV,avoiding: :exhaust @15:16:03.27 CBV.dial between SSV and waste2 @15:16:03.70 CSV.dial between air and CTV @15:16:04.03 CS.seek 8.273ml @15:16:09.01 CTV.dial CSV,avoiding: :air @15:16:09.04
CSV.dial CTV @15:16:09.49 CS.reconfigure CSpressurize @15:16:09.54 CS.seek 0ml @15:16:31.35 CSV.dial :air @15:16:31.82 CS.reconfigure CSfast @15:16:31.87 CS.seek 10ml @15:16:56.01 CSV.dial between air and CTV @15:16:56.36 CS.seek 3.127ml @15:17:13.20 CTV.dial CSV,avoiding: :air @15:17:13.23
CSV.dial CTV @15:17:13.55 CS.reconfigure CSpressurize @15:17:13.60 CS.seek 0ml @15:17:21.57 BubblePoint: Intake @68.4psia, Outlet @12.7psia @15:17:21.58 CBV.dial SSV @15:17:21.93 SS.reconfigure SSconfig @15:17:21.99 SS.seek 10ml @15:17:27.33 delay 15 seconds @15:17:42.48 Evacuated: Intake @35.7psia, Outlet @15.7psia @15:17:42.50 SSV.dial :spit,avoiding: :exhaust @15:17:43.88 SS.reconfigure SSexhale @15:17:43.94 SS.seek 0ml @15:17:49.17 SS.reconfigure SSfast @15:17:49.22 SS.seek 5ml @15:17:51.91 SSV.dial CBV,avoiding: :exhaust @15:17:51.94
CBV.dial SSV @15:17:53.35 CTV.dial :air,avoiding: :intake @15:17:54.74 CSV.dial CTV @15:17:54.79
SS.seek 10ml @15:17:55.78 CSV.dial :air @15:17:56.40 CS.reconfigure CSfast @15:17:56.45 CS.seek 0ml @15:17:57.35
SSV.dial :spit,avoiding: :exhaust @15:17:58.00 CS.coast @15:17:58.74
SS.reconfigure SSexhale @15:17:58.79 SS.seek 0ml @15:18:04.06 SS.reconfigure SSfast @15:18:04.11 SS.seek 5ml @15:18:06.60 SSV.dial CBV,avoiding: :exhaust @15:18:06.63
CBV.dial SSV @15:18:09.04 SSV.dial :spit,avoiding: :exhaust @15:18:10.41 SS.reconfigure SSconfig @15:18:10.46 SS.seek 0ml @15:18:13.60 SS.coast @15:18:13.65 Lysing: Adding 1.5ml lysis @15:18:13.66 CBV.dial between CSV and waste1,avoiding: :DBV @15:18:15.23 CTV.dial CSV @15:18:17.13 CSV.dial CSR @15:18:17.96 Pulling 1ml of CSR.lysis @15:18:17.97 CSR.select :lysis @15:18:18.10 CS.reconfigure CSconfig @15:18:18.15 CS.seek 1ml @15:18:21.73 delay 5 seconds @15:18:26.75 CSR.select :air @15:18:26.94 CS.seek 6ml @15:18:40.63 delay 2 seconds @15:18:42.65 CSV.dial :waste1 @15:18:43.99 CS.reconfigure CSfast @15:18:44.04 CS.seek :empty @15:18:58.78 CSV.dial CSR @15:19:00.20 Pulling 1.5ml of CSR.lysis @15:19:00.21 CSR.select :lysis @15:19:00.34 CS.reconfigure CSconfig @15:19:00.38 CS.seek 1.5ml @15:19:05.23 delay 5 seconds @15:19:10.25 CSR.select :air @15:19:10.44 CS.seek 4.5ml @15:19:19.10 delay 2 seconds @15:19:21.12 CSV.dial CTV @15:19:21.61 CS.seek 1.275ml @15:19:30.93 Heating sh1 puck from tube 3 in Collection Clamp to 85C for 5:00 @15:19:30.95 CH.seek 85C,8:00 @15:22:31.10 CH.hold 5:00,85C @15:27:31.28 Collection Puck Heater at 85C after lysing @15:27:31.30 CS.seek 0ml @15:27:35.77 CTV.dial between spare and air,avoiding: :intake @15:27:36.87 CSV.dial :air @15:27:37.34 CS.seek 5ml @15:27:51.04 CSV.dial CTV @15:27:52.10 delay 2 seconds @15:27:54.13 CSV.dial :air @15:27:54.61 delay 2 seconds @15:27:56.64 CSV.dial CTV @15:27:57.13 delay 2 seconds @15:27:59.16 CSV.dial :air @15:27:59.64 delay 2 seconds @15:28:01.67 CS.seek 0ml @15:28:15.42 Recovering Lysate into Collection syringe @15:28:15.43 CSV.dial CBV @15:28:16.49 CBV.dial CSV @15:28:16.90 CS.seek 5ml @15:28:30.61 delay 5 seconds @15:28:35.63 CTV.dial :air @15:28:36.20 CS.seek 7ml @15:28:42.33 delay 5 seconds @15:28:47.35 CSV.dial :air2 @15:28:47.84 CS.seek 10ml @15:28:56.49 CSV.dial between air and CTV,avoiding: CBV @15:28:57.82 Lysing Complete @15:28:57.90 Storing sh1 puck in Collection Clamp to tube 2 @15:28:58.10 SC.reconfigure SCtight @15:28:58.15 SC.seek 2 @15:29:00.25 SC.reconfigure SCloose @15:29:00.48 Elbow.reconfigure TightElbow @15:29:00.53 Elbow.seek 2 @15:29:02.36 Forearm.reconfigure TightForearm @15:29:02.41 Forearm.seek 2 + 700 counts @15:29:03.43 SE.reconfigure SEpick @15:29:03.50 SE.seek beamBreak @15:29:06.19 SE.configure SEconfig @15:29:06.36 Elbow.reconfigure LimpElbow @15:29:06.41 Forearm.reconfigure LooseForearm @15:29:06.46 Forearm.seek 2 @15:29:06.77 Elbow.hold @15:29:06.84 Elbow.reconfigure TightElbow @15:29:06.95 SE.seek 4.07 Pucks below Top Plate @15:29:07.44 Forearm.force -20 @15:29:07.48 Forearm.seek 2 + 331 counts @15:29:07.82 Forearm.reconfigure TightForearm @15:29:07.87 Forearm.seek 2 + 700 counts @15:29:08.30 SE.seek 5.07 Pucks below Top Plate @15:29:09.03 Forearm.reconfigure LooseForearm @15:29:09.08 Forearm.seek :retract @15:29:10.00 Elbow.seek CC @15:29:11.74 Elbow.reconfigure LooseElbow @15:29:11.85 Forearm.seek CC @15:29:12.83 Hand.close @15:29:13.46 CC.seek :open @15:29:18.44 Forearm.seek :retract @15:29:19.50 Elbow.reconfigure TightElbow @15:29:19.55 Elbow.seek 2 @15:29:21.42 Elbow.reconfigure LimpElbow @15:29:21.48 Forearm.seek 2 @15:29:22.50 Elbow.hold @15:29:22.57 Elbow.reconfigure TightElbow @15:29:22.63 Hand.open @15:29:23.34 Forearm.force -20 @15:29:23.39 Forearm.seek 2 + 337 counts @15:29:23.72 Forearm.reconfigure TightForearm @15:29:23.78 Forearm.seek 2 + 700 counts @15:29:24.27 SE.seek :down @15:29:25.42
Forearm.reconfigure LooseForearm @15:29:25.47 Forearm.seek :retract @15:29:26.59 SE.coast @15:29:26.63
SC.coast @15:29:26.67 Tranferring lysate to PRV @15:29:26.68 CSV.dial :air @15:29:27.03 CS.seek 10ml @15:29:27.11 CSV.dial between air and CTV @15:29:27.46 AIV.dial :bypass @15:29:27.66 Beginning samplePassC @15:29:27.68 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:29:27.75 Hand.open @15:29:28.10 Elbow.seek FlushPuck::Garage @15:29:30.06 Elbow.reconfigure LooseElbow @15:29:30.17 Forearm.seek :garage @15:29:31.18 Hand.close @15:29:31.96 Forearm.seek :retract @15:29:32.97 Elbow.reconfigure TightElbow @15:29:33.02 Elbow.seek CC @15:29:35.89 Elbow.reconfigure LooseElbow @15:29:36.00 Forearm.reconfigure TightForearm @15:29:36.05 Forearm.seek CC @15:29:37.33 CC.seek :closed @15:29:41.58 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.02 @15:29:41.66 Hand.open @15:29:42.40 Forearm.reconfigure LooseForearm @15:29:42.45 Forearm.seek :retract @15:29:43.42 AWV.select :air @15:29:43.44 AIV.dial :bypass @15:29:43.45 PSV.dial PSR @15:29:45.44 Pulling 1.5ml of PSR.flush @15:29:45.45 PSR.select :flush @15:29:45.58 PS.seek 1.5ml @15:30:01.61 delay 5 seconds @15:30:06.64 PSV.dial :waste1 @15:30:07.14 PS.seek 0ml @15:30:23.13 PSV.dial PSR @15:30:23.61 PSR.select :air @15:30:23.74 PS.seek 2.5ml @15:30:49.83 PRV.dial CBV @15:30:49.86
CBV.dial PRV @15:30:50.74 PSV.dial :mixloop @15:30:51.80 CTV.dial :air,avoiding: :intake @15:30:51.81 PTV.dial :PRVmixing @15:30:51.90 PS.seek 0ml @15:31:18.10 PSV.dial :air @15:31:18.59 PS.seek 2.5ml @15:31:44.73 PSV.dial :mixloop @15:31:45.23 PS.seek 0ml @15:32:11.32 PSV.dial :air @15:32:11.81 PS.seek 2.5ml @15:32:37.89 PSV.dial :mixloop @15:32:38.39 PS.seek 0ml @15:33:04.49 Garaging FlushPuck in Collection Clamp @15:33:04.57 Elbow.reconfigure TightElbow @15:33:04.62 Elbow.seek CC @15:33:04.88 Elbow.reconfigure LooseElbow @15:33:04.98 Forearm.seek CC @15:33:05.96 Hand.close @15:33:06.59 CC.seek :open @15:33:10.97 Forearm.seek :retract @15:33:11.93 Elbow.reconfigure TightElbow @15:33:11.98 Elbow.seek :garage @15:33:15.05 Elbow.reconfigure LooseElbow @15:33:15.16 Forearm.reconfigure TightForearm @15:33:15.21 Forearm.seek :garage @15:33:16.38 Hand.open @15:33:17.03 Forearm.reconfigure LooseForearm @15:33:17.08 Forearm.seek :retract @15:33:18.14 Connecting PS to CS to transfer sample @15:33:18.15 CBV.dial AIV @15:33:18.16 AIV.dial :bypass @15:33:18.17 PSV.dial :mixloop @15:33:18.19 PTV.dial :PRVmixing @15:33:18.20 PRV.dial AIV @15:33:18.21 CTV.dial :air,avoiding: :intake @15:33:18.23 Loading lyfil puck from tube 3 into Collection Clamp @15:33:18.50 SC.reconfigure SCtight @15:33:18.54 SC.seek 3 @15:33:20.66 SC.reconfigure SCloose @15:33:20.89 Elbow.reconfigure TightElbow @15:33:20.94 Elbow.seek 3 @15:33:22.98 Forearm.reconfigure TightForearm @15:33:23.04 Forearm.seek 3 + 700 counts @15:33:24.11 SE.reconfigure SEpick @15:33:24.18 SE.seek beamBreak @15:33:25.90 SE.configure SEconfig @15:33:26.08 Elbow.seek 3 @15:33:27.03 Forearm.seek 3 + 300 counts @15:33:27.28 Forearm.reconfigure LooseForearm @15:33:27.33 Hand.close @15:33:27.99 SE.seek :down @15:33:28.22
Forearm.seek :retract @15:33:29.15 Elbow.seek CC @15:33:29.39 SE.coast @15:33:30.80
Elbow.reconfigure LooseElbow @15:33:30.91 Forearm.reconfigure TightForearm @15:33:30.96 Forearm.seek CC @15:33:32.24 CC.seek :closed @15:33:36.52 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.09 @15:33:36.53 Hand.open @15:33:37.28 Forearm.reconfigure LooseForearm @15:33:37.33 Forearm.seek :retract @15:33:38.31 SC.coast @15:33:38.35 CTV.dial CSV,avoiding: :intake @15:33:39.74 CSV.dial CTV @15:33:40.17 CS.seek 5ml @15:33:54.09 delay 1:20 @15:35:14.12 CS.seek 2.5ml @15:35:21.49 delay 40 seconds @15:36:01.52 CS.seek 0ml @15:36:08.96 delay 1:00 @15:37:08.98 PSV.dial :air @15:37:09.47 PS.seek 2.5ml @15:37:35.64 PSV.dial :mixloop @15:37:36.13 delay 3 seconds @15:37:39.15 PSV.dial :air @15:37:39.63 delay 2 seconds @15:37:41.64 PSV.dial :mixloop @15:37:42.12 delay 3 seconds @15:37:45.14 PSV.dial :waste1 @15:37:46.49 PS.seek :empty @15:38:12.64 CBV.dial :waste1 @15:38:13.14 delay 5 seconds @15:38:18.16 CBV.dial AIV @15:38:18.67 delay 10 seconds @15:38:28.69 CBV.dial :waste1 @15:38:29.20 delay 10 seconds @15:38:39.22 CBV.dial AIV @15:38:39.72 delay 10 seconds @15:38:49.75 CTV.dial CSV,avoiding: :intake @15:38:49.76 CBV.dial between AIV and waste1 @15:38:50.12 CSV.dial :air @15:38:50.60 CS.seek 10ml @15:39:17.08 CSV.dial CTV @15:39:17.57 CS.seek 0ml @15:39:44.14 CBV.dial :waste1 @15:39:44.48 delay 10 seconds @15:39:54.52 CTV.dial :air,avoiding: :intake @15:39:55.96 Storing lyfil puck in Collection Clamp to tube 2 @15:39:56.15 SC.reconfigure SCtight @15:39:56.20 SC.seek 2 @15:39:58.34 SC.reconfigure SCloose @15:39:58.57 Elbow.reconfigure TightElbow @15:39:58.61 Elbow.seek 2 @15:40:00.91 Forearm.reconfigure TightForearm @15:40:01.05 Forearm.seek 2 + 700 counts @15:40:02.06 SE.reconfigure SEpick @15:40:02.13 SE.seek beamBreak @15:40:04.67 SE.configure SEconfig @15:40:04.84 Elbow.reconfigure LimpElbow @15:40:04.90 Forearm.reconfigure LooseForearm @15:40:04.94 Forearm.seek 2 @15:40:05.25 Elbow.hold @15:40:05.33 Elbow.reconfigure TightElbow @15:40:05.45 SE.seek 5.07 Pucks below Top Plate @15:40:05.91 Forearm.force -20 @15:40:05.95 Forearm.seek 2 + 336 counts @15:40:06.28 Forearm.reconfigure TightForearm @15:40:06.33 Forearm.seek 2 + 700 counts @15:40:06.62 SE.seek 6.07 Pucks below Top Plate @15:40:07.31 Forearm.reconfigure LooseForearm @15:40:07.36 Forearm.seek :retract @15:40:08.30 Elbow.seek CC @15:40:10.06 Elbow.reconfigure LooseElbow @15:40:10.16 Forearm.seek CC @15:40:11.12 Hand.close @15:40:11.72 CC.seek :open @15:40:16.24 Forearm.seek :retract @15:40:17.22 Elbow.reconfigure TightElbow @15:40:17.27 Elbow.seek 2 @15:40:19.17 Elbow.reconfigure LimpElbow @15:40:19.22 Forearm.seek 2 @15:40:20.22 Elbow.hold @15:40:20.29 Elbow.reconfigure TightElbow @15:40:20.34 Hand.open @15:40:21.10 Forearm.force -20 @15:40:21.14 Forearm.seek 2 + 343 counts @15:40:21.49 Forearm.reconfigure TightForearm @15:40:21.54 Forearm.seek 2 + 700 counts @15:40:21.85 SE.seek :down @15:40:23.02
Forearm.reconfigure LooseForearm @15:40:23.07 Forearm.seek :retract @15:40:24.08 SE.coast @15:40:24.12
SC.coast @15:40:24.16 PSV.dial :air @15:40:25.30 PS.seek 0.499ml @15:40:31.18 delay 3 seconds @15:40:34.19 PSV.dial :mixloop @15:40:34.67 PRV.dial :air @15:40:35.15 delay 3 seconds @15:40:38.17 PRV.dial CBV @15:40:38.65 CBV.dial AIV @15:40:39.14 PRV.dial between CBV and air @15:40:39.48 PRV.dial :air @15:40:39.88 PS.reconfigure PSslow @15:40:39.93 PS.seek 0.599ml @15:40:42.92 PRV.dial :waste2 @15:40:43.69 PS.reconfigure PSconfig @15:40:43.74 PS.seek :empty @15:40:50.65 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:40:50.73 Hand.open @15:40:51.10 Elbow.seek FlushPuck::Garage @15:40:53.04 Elbow.reconfigure LooseElbow @15:40:53.14 Forearm.seek :garage @15:40:54.19 Hand.close @15:40:54.98 Forearm.seek :retract @15:40:55.99 Elbow.reconfigure TightElbow @15:40:56.04 Elbow.seek CC @15:40:58.88 Elbow.reconfigure LooseElbow @15:40:58.99 Forearm.reconfigure TightForearm @15:40:59.04 Forearm.seek CC @15:41:00.35 CC.seek :closed @15:41:04.62 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.05 @15:41:04.69 Hand.open @15:41:05.42 Forearm.reconfigure LooseForearm @15:41:05.47 Forearm.seek :retract @15:41:06.51 Rinsing MixLoop with GuSCN @15:41:06.52 PSV.dial :mixloop,avoiding: PBV @15:41:06.53 PTV.dial :PRVmixing,avoiding: :puck @15:41:06.55 PRV.dial :spare1 @15:41:07.69 PS.seek 1.099ml @15:41:19.71 delay 2 seconds @15:41:21.73 PRV.dial :air @15:41:22.78 PS.seek 2.5ml @15:41:37.78 PSV.dial :waste1,avoiding: PBV @15:41:39.14 PS.seek :empty @15:42:05.28 Diluting Lysate in Syringe @15:42:05.29 PSV.dial :PTV,avoiding: PBV @15:42:06.93 PTV.dial :PRV,avoiding: :puck @15:42:07.70 CBV.dial :PRV @15:42:07.71 PRV.dial :air @15:42:07.72 delay 2 seconds @15:42:09.75 PRV.dial CBV @15:42:10.25 delay 2 seconds @15:42:12.27 PS.reconfigure PSslow1 @15:42:12.33 PS.seek 0.1ml @15:42:23.59 delay 5 seconds @15:42:28.61 PRV.dial :spare1 @15:42:29.39 PS.reconfigure PSconfig @15:42:29.44 PS.seek 1ml @15:42:39.37 delay 2 seconds @15:42:41.39 PRV.dial :air @15:42:42.47 PS.seek 2.5ml @15:42:58.46 delay 2 seconds @15:43:00.48 Parking diluted lysate in Mixing Coil @15:43:00.49 PSV.dial :mixloop @15:43:00.97 PTV.dial :PRVmixing,avoiding: :puck @15:43:01.75 PRV.dial :CBV @15:43:02.32 PS.seek 1.5ml @15:43:13.34 delay 2 seconds @15:43:15.36 PSV.dial :waste1,avoiding: PBV @15:43:16.72 PS.seek :empty @15:43:32.75 Beginning samplePassF @15:43:32.83 AWV.select :air @15:43:33.38 AIV.dial :bypass @15:43:33.41 PSV.dial PSR,avoiding: PBV @15:43:33.97 Pulling 1.5ml of PSR.flush @15:43:33.98 PSR.select :flush @15:43:34.11 PS.seek 1.5ml @15:43:50.06 delay 5 seconds @15:43:55.09 PSV.dial :waste1 @15:43:55.58 PS.seek 0ml @15:44:11.58 PSV.dial PSR @15:44:12.07 PSR.select :air @15:44:12.20 PS.seek 2.5ml @15:44:38.34 PRV.dial CBV @15:44:38.37
CBV.dial PRV @15:44:38.39 PSV.dial :PTV,via: :air @15:44:40.45 CTV.dial :air,avoiding: :intake @15:44:40.46 PTV.dial :PRV,avoiding: :puck @15:44:41.25 PS.seek 0ml @15:45:07.36 PSV.dial :air @15:45:08.12 PS.seek 2.5ml @15:45:34.43 PSV.dial :PTV @15:45:35.33 PS.seek 0ml @15:46:01.57 moving diluted lysate into CBV-AIV line @15:46:01.58 PSV.dial :air @15:46:02.34 PS.seek 2.5ml @15:46:28.50 delay 2 seconds @15:46:30.51 PSV.dial :mixloop @15:46:30.98 PTV.dial :PRVmixing,avoiding: :puck @15:46:31.76 PRV.dial CBV @15:46:31.92 PS.seek 1.8ml @15:46:39.84 delay 3 seconds @15:46:42.85 PRV.dial :waste2 @15:46:43.94 PS.seek :empty @15:47:03.03 Clearing PML for modified lysate @15:47:03.04 CTV.dial :air,avoiding: :intake @15:47:03.05 PRV.dial :waste2 @15:47:03.07 PTV.dial :PRV,avoiding: :puck @15:47:03.83 delay 2 seconds @15:47:05.85 PTV.dial :PRVmixing,avoiding: :puck @15:47:06.62 delay 2 seconds @15:47:08.64 PSV.dial :PML @15:47:08.67 delay 2 seconds @15:47:10.69 PRV.dial :air @15:47:11.47 delay 2 seconds @15:47:13.55 PS.seek 1.499ml @15:47:29.53 delay 5 seconds @15:47:34.56 PS.seek 2.5ml @15:47:45.58 PSV.dial :air @15:47:46.05 PS.seek 0ml @15:48:12.16 mixing lysate with speDil @15:48:12.17 PSV.dial :PML @15:48:12.64 CBV.dial :PRV @15:48:12.65 PRV.dial :air @15:48:12.66 delay 2 seconds @15:48:14.68 PRV.dial :speDil @15:48:15.19 delay 2 seconds @15:48:17.28 PS.reconfigure PSslow1 @15:48:17.34 PS.seek 0.225ml @15:48:41.12 delay 5 seconds @15:48:46.14 PRV.dial CBV @15:48:46.94 delay 2 seconds @15:48:49.02 PS.seek 0.475ml @15:49:14.78 delay 5 seconds @15:49:19.80 PRV.dial :air @15:49:20.30 delay 2 seconds @15:49:22.32 PS.seek 0.965ml @15:50:12.28 delay 10 seconds @15:50:22.31 PRV.dial CBV @15:50:22.78 CTV.dial :air,avoiding: :intake @15:50:22.79 PSV.dial :air @15:50:23.25 PS.reconfigure PSconfig @15:50:23.30 PS.seek 2.5ml @15:50:39.59 delay 3 seconds @15:50:42.61 PSV.dial PTV @15:50:43.36 PTV.dial :PRV,avoiding: :puck @15:50:44.15 PS.seek 0ml @15:51:10.30 PSV.dial :air @15:51:11.06 PS.seek 2.5ml @15:51:37.16 delay 3 seconds @15:51:40.17 PSV.dial PTV @15:51:40.94 PTV.dial :PRV,avoiding: :puck @15:51:40.96 PS.seek 0ml @15:52:07.03 Pushing modified lysate into AIV Injection Loop @15:52:07.57 CBV.dial AIV @15:52:07.60 AIV.dial :bypass @15:52:07.61 PRV.dial CBV @15:52:07.63 PSV.dial :air @15:52:08.42 PS.seek 2.5ml @15:52:34.53 delay 3 seconds @15:52:37.55 PSV.dial :PML @15:52:38.03 PTV.dial :PRVmixing,avoiding: :puck @15:52:38.89 PS.reconfigure PSslow1 @15:52:38.94 PS.seek 1.74ml @15:53:56.50 delay 5 seconds @15:54:01.53 AIV.dial :sample @15:54:02.12 AWV.select :waste @15:54:02.29 PS.reconfigure PSslow2 @15:54:02.34 PS.seek 1.22ml @15:55:50.97 delay 15 seconds @15:56:05.99 AIV.dial :bypass @15:56:06.59 PS.reconfigure PSconfig @15:56:06.64 PS.seek 0ml @15:56:19.87 PSV.dial :air @15:56:20.37 PS.seek 2.5ml @15:56:46.53 PSV.dial :PML @15:56:47.03 PS.seek 0ml @15:57:13.21 AIV.dial :coilToMFB @15:57:13.23 AWV.select :air @15:57:13.40 Extracting Nucleic Acid from AIV @15:57:13.43
Beginning samplePassD @15:57:13.50 SPE.hold 2:46:40,55C @15:57:13.55 ASV.dial :SPEdelivery @15:57:13.63
AWV.select :air @15:57:13.65 AIV.dial :bypass @15:57:13.66 PSV.dial PSR @15:57:13.95 delay 20 seconds @15:57:14.83
Pulling 1.5ml of PSR.killMFB @15:57:14.84 PSR.select :killMFB @15:57:14.97 PS.seek 1.5ml @15:57:31.10 delay 5 seconds @15:57:33.98 primeLysateINT @15:57:33.99 AWV.select :air @15:57:34.00 AIV.dial :coilToMFB @15:57:34.02 ATV.dial AIV @15:57:35.70 AS.reconfigure ASslow1 @15:57:35.75 AS.seek 0.299ml @15:57:36.13
PSV.dial :waste1 @15:57:36.62 PS.seek 0ml @15:57:52.65 PSV.dial PSR @15:57:53.15 PSR.select :air @15:57:53.28 PS.seek 2.5ml @15:58:19.44 PRV.dial CBV @15:58:19.47
CBV.dial PRV @15:58:19.48 PSV.dial :mixloop @15:58:20.69 CTV.dial :air,avoiding: :intake @15:58:20.70 PTV.dial :PRVmixing @15:58:20.72 PS.seek 0ml @15:58:36.40 delay 10 seconds @15:58:46.42 ATV.dial :air2 @15:58:46.90
PSV.dial PSR @15:58:47.25 AS.seek 0.329ml @15:58:48.05
Pulling 1.5ml of PSR.flush @15:58:48.06 PSR.select :flush @15:58:48.19 PS.seek 1.5ml @15:58:53.56 ATV.dial :waste2 @15:58:54.12 AS.reconfigure ASnorm @15:58:54.17 AS.seek :empty @15:59:04.29
delay 5 seconds @15:59:09.31 PSV.dial :waste1 @15:59:09.83 PS.seek 0ml @15:59:11.20 Lysate primed! @15:59:11.21 AFV.select :holdingCoil @15:59:11.22 ATV.dial :air2 @15:59:11.85 AS.reconfigure ASslow1 @15:59:11.90 AS.seek 0.069ml @15:59:25.93
PSV.dial PSR @15:59:26.28 delay 3 seconds @15:59:26.43
PSR.select :air @15:59:26.56 PS.seek 2.5ml @15:59:29.30 ATV.dial :AIV @15:59:30.14 AS.seek 0.469ml @15:59:53.30 PRV.dial CBV @15:59:53.34
CBV.dial PRV @15:59:53.36 PSV.dial :mixloop @15:59:54.46 CTV.dial :air,avoiding: :intake @15:59:54.47 PTV.dial :PRVmixing @15:59:54.50 PS.seek 0ml @16:00:20.69 PSV.dial PSR @16:00:21.84 Pulling 1.5ml of PSR.flush PSR.select :flush @16:00:21.97 PS.seek 1.5ml @16:00:37.94 delay 5 seconds @16:00:42.97 PSV.dial :waste1 @16:00:43.46 PS.seek 0ml @16:00:50.87 delay 5 seconds @16:00:55.95 Solid Phase Extraction Heater at 49C @16:00:55.97 ATV.dial ASV @16:00:56.35 ASV.dial :SPEdelivery @16:00:56.44 AS.seek 0.041ml @16:00:59.50
PSV.dial PSR @16:01:00.12 PSR.select :air @16:01:00.25 PS.seek 2.5ml @16:01:26.45 PRV.dial CBV @16:01:26.48
CBV.dial PRV @16:01:26.49 PSV.dial :mixloop @16:01:27.59 CTV.dial :air,avoiding: :intake @16:01:27.60 PTV.dial :PRVmixing @16:01:27.62 PS.seek 0ml @16:01:53.81 PSV.dial PSR @16:01:54.96 Pulling 1.5ml of PSR.flush @16:01:54.97 PSR.select :flush @16:01:55.10 PS.seek 1.5ml @16:02:11.23 delay 5 seconds @16:02:16.25 PSV.dial :waste1 @16:02:16.75 PS.seek 0ml @16:02:22.78 delay 10 seconds @16:02:32.75
PSV.dial PSR @16:02:32.82 rinseLysateLineINT @16:02:32.83 AFV.select :carrierSolution @16:02:32.93 delay 2 seconds @16:02:33.24
PSR.select :air @16:02:33.37 PS.seek 2.5ml @16:02:34.96 AS.reconfigure ASconfig @16:02:35.01 AS.seek 1ml @16:02:59.48 PRV.dial CBV @16:02:59.51
CBV.dial PRV @16:02:59.52 PSV.dial :mixloop @16:03:00.60 CTV.dial :air,avoiding: :intake @16:03:00.61 PTV.dial :PRVmixing @16:03:00.63 PS.reconfigure PSslow @16:03:00.68 PS.seek 1.2ml @16:03:27.51 PSV.dial :waste1 @16:03:28.90 PS.reconfigure PSconfig @16:03:28.95 PS.seek 0ml @16:03:30.40 delay 1 second @16:03:31.41 AFV.select :holdingCoil @16:03:31.55 delay 1 second @16:03:32.56 ATV.dial AIV @16:03:33.11 AIV.dial :coilToMFB @16:03:33.12 AWV.select :waste @16:03:33.23 AS.reconfigure ASslow0 @16:03:33.28 AS.seek 0ml @16:03:41.91
PSV.dial :air @16:03:43.13 PS.seek 2.5ml @16:04:09.31 PSV.dial :mixloop @16:04:09.79 PS.reconfigure PSslow @16:04:09.84 PS.seek 0ml @16:05:00.88 PSV.dial :air @16:05:01.37 PS.reconfigure PSconfig @16:05:01.42 PS.seek 2.5ml @16:05:14.17 ATV.dial :air2 @16:05:14.82 AS.reconfigure ASslow1 @16:05:14.87 AS.seek 0.05ml @16:05:25.19 ATV.dial :AIV @16:05:26.00 AS.seek 0.01ml @16:05:27.56
PSV.dial :mixloop @16:05:28.04 PS.reconfigure PSslow @16:05:28.09 PS.seek 0ml @16:05:34.32 ATV.dial :waste @16:05:36.43 AS.seek :empty @16:05:38.89 ethanol wash @16:05:38.90 ATV.dial :air2 @16:05:40.10 AS.reconfigure ASslow2 @16:05:40.15 AS.seek 0.009ml @16:05:50.47 delay 3 seconds @16:05:53.50 Pulling 0.4ml of Ethanol Wash @16:05:53.51 ATV.dial :ethanol @16:05:54.30 AS.reconfigure ASslow1 @16:05:54.35 AS.seek 0.409ml @16:06:19.23
Cleaning Collection Syringe @16:06:19.24 CSV.dial CSR @16:06:19.77 Pulling 4ml of CSR.flush @16:06:19.78 CSR.select :flush @16:06:19.91 CS.seek 4ml @16:06:31.24 delay 5 seconds @16:06:36.26 CSR.select :air @16:06:36.40 CS.seek 10ml @16:06:52.80 CSV.dial CTV @16:06:52.83
CTV.dial CSV @16:06:54.29 CBV.dial :waste1 @16:06:54.81 CS.seek 0ml @16:07:15.03 delay 3 seconds @16:07:18.05 ATV.dial :air1 @16:07:18.47 delay 3 seconds @16:07:21.32
CSV.dial CSR @16:07:21.56 AS.seek 0.434ml @16:07:21.87
Pulling 4ml of CSR.flush @16:07:21.88 CSR.select :flush @16:07:22.00 CS.seek 4ml @16:07:26.87 delay 3 seconds @16:07:29.89 ATV.dial :ethanol @16:07:30.49 AS.seek 0.419ml @16:07:33.30
delay 5 seconds @16:07:33.79 delay 3 seconds @16:07:36.81 ATV.dial ASV @16:07:38.07 AS.seek 0.092ml @16:07:38.32
CSR.select :air @16:07:38.46 CS.seek 10ml @16:07:54.83 CSV.dial CTV @16:07:54.86
CTV.dial CSV @16:07:55.35 CBV.dial :waste1 @16:07:55.37 CS.seek 0ml @16:08:21.94 Garaging FlushPuck in Collection Clamp @16:08:22.02 Elbow.reconfigure TightElbow @16:08:22.06 Elbow.seek CC @16:08:22.35 Elbow.reconfigure LooseElbow @16:08:22.45 Forearm.seek CC @16:08:23.44 Hand.close @16:08:24.01 CC.seek :open @16:08:28.37 Forearm.seek :retract @16:08:29.37 Elbow.reconfigure TightElbow @16:08:29.42 Elbow.seek :garage @16:08:32.46 Elbow.reconfigure LooseElbow @16:08:32.57 Forearm.reconfigure TightForearm @16:08:32.62 Forearm.seek :garage @16:08:33.79 Hand.open @16:08:34.46 Forearm.reconfigure LooseForearm @16:08:34.51 Forearm.seek :retract @16:08:35.53 Whole Cell Archive sampling 1500ml @16:08:35.55 Loading wcr puck from tube 3 into Collection Clamp @16:08:35.82 SC.reconfigure SCtight @16:08:35.88 SC.seek 3 @16:08:38.06 SC.reconfigure SCloose @16:08:38.88 Elbow.reconfigure TightElbow @16:08:38.93 Elbow.seek 3 @16:08:41.01 Forearm.reconfigure TightForearm @16:08:41.07 Forearm.seek 3 + 700 counts @16:08:42.13 SE.reconfigure SEpick @16:08:42.21 SE.seek beamBreak @16:08:43.88 SE.configure SEconfig @16:08:44.08 delay 3 seconds @16:08:44.10 Elbow.seek 3 @16:08:44.46 Forearm.seek 3 + 300 counts @16:08:44.70 Forearm.reconfigure LooseForearm @16:08:44.75 Hand.close @16:08:45.41 SE.seek :down @16:08:45.64 Forearm.seek :retract @16:08:46.61 Elbow.seek CC @16:08:46.93 SE.coast @16:08:47.10 ATV.dial :waste2 @16:08:47.83 AS.reconfigure ASconfig @16:08:47.88 AS.seek :empty @16:08:48.33 Elbow.reconfigure LooseElbow @16:08:48.45 Forearm.reconfigure TightForearm @16:08:48.50 Forearm.seek CC @16:08:49.81 CC.seek :closed @16:08:53.56 ATV.dial :air2 @16:08:53.97 AS.reconfigure ASslow1 @16:08:54.11 AS.seek 1ml @16:08:54.15 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.09 @16:08:54.19 Hand.open @16:08:54.97 Forearm.reconfigure LooseForearm @16:08:55.02 Forearm.seek :retract @16:08:56.00 SC.coast @16:08:56.04 collectSample 1500ml w/bubble point of 55psi @16:08:56.06 Priming 7ml @16:08:56.07 Intake.select :close @16:08:56.08 Exhaust.select :close @16:08:56.10 SS.hold @16:08:56.34 Calibrating pressure sensors @16:08:56.35 CTV.dial :air @16:08:57.74 SSV.dial :spit,avoiding: :exhaust @16:08:57.76 SS.reconfigure SSfast @16:08:57.81 SS.seek 5ml @16:09:00.39 SSV.dial CBV @16:09:00.42 CBV.dial SSV @16:09:01.91 delay 3.8 seconds @16:09:05.87 SSV.dial :spit @16:09:07.28 SS.seek 0ml @16:09:09.90 SSV.dial CBV @16:09:11.30 CTV.dial :intake @16:09:12.68 SSV.dial CBV @16:09:12.71 CBV.dial SSV @16:09:12.74 Allowing 40s for 7.0ml of remaining 7.0ml @16:09:12.75 Intake.select :open @16:09:14.93 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:09:14.95 SS.reconfigure SSinhale.with maxSpeed: 150 @16:09:15.01 SS.seek 7ml @16:09:18.46 Vacuum too high -- waiting up to 40s @16:09:20.61 Intake @33.4psia, vacuum from 20.0 to 24.0psi @16:09:20.63 SS.reconfigure SSinhale.with maxSpeed: 135 @16:09:20.68 SS.seek 7ml @16:09:23.98 Stroke completed -- waiting for pressure to equalize ... @16:09:29.17 Intake.select :close @16:09:29.34 SSV.dial :exhaust @16:09:30.16 Exhaust.select :open @16:09:30.27 SS.reconfigure SSexhale @16:09:30.33 SS.seek 0ml @16:09:33.98 Exhaust.select :close @16:09:34.11 Sampling 1500.0ml @16:09:34.13 Intake.select :close @16:09:34.14 Exhaust.select :close @16:09:34.15 SS.reconfigure SSconfig @16:09:34.20 SS.hold @16:09:34.42 CTV.dial :intake @16:09:34.45 SSV.dial CBV @16:09:34.47 CBV.dial SSV @16:09:35.32 Allowing 130s for 25.0ml of remaining 1500.0ml @16:09:35.33 Intake.select :open @16:09:37.52 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:09:37.54 SS.reconfigure SSinhale.with maxSpeed: 135 @16:09:37.59 SS.seek 25ml @16:09:41.73 Vacuum too high -- waiting up to 40s @16:09:43.63 Intake @33.6psia, vacuum from 20.0 to 24.0psi @16:09:43.65 SS.reconfigure SSinhale.with maxSpeed: 122 @16:09:43.71 SS.seek 25ml @16:09:46.58 Vacuum too high -- waiting up to 40s @16:09:48.47 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:09:48.49 SS.reconfigure SSinhale.with maxSpeed: 110 @16:09:48.54 SS.seek 25ml @16:09:51.26 Vacuum too high -- waiting up to 40s @16:09:52.72 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:09:52.74 SS.reconfigure SSinhale.with maxSpeed: 99 @16:09:52.80 SS.seek 25ml @16:09:55.31 Vacuum too high -- waiting up to 40s @16:09:56.96 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:09:56.98 SS.reconfigure SSinhale.with maxSpeed: 89 @16:09:57.03 SS.seek 25ml @16:10:00.06 Vacuum too high -- waiting up to 40s @16:10:01.44 SS.reconfigure SSinhale.with maxSpeed: 80 @16:10:01.50 SS.seek 25ml @16:10:04.95 Vacuum too high -- waiting up to 40s @16:10:06.32 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:10:06.35 SS.reconfigure SSinhale.with maxSpeed: 72 @16:10:06.40 SS.seek 25ml @16:10:09.92 Vacuum too high -- waiting up to 40s @16:10:11.30 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:10:11.32 SS.reconfigure SSinhale.with maxSpeed: 65 @16:10:11.38 SS.seek 25ml @16:10:15.36 Vacuum too high -- waiting up to 40s @16:10:16.47 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:10:16.49 SS.reconfigure SSinhale.with maxSpeed: 59 @16:10:16.54 SS.seek 25ml @16:10:17.23 Stroke completed -- waiting for pressure to equalize ... @16:10:19.21 Intake.select :close @16:10:19.39 SSV.dial :exhaust @16:10:20.22 Exhaust.select :open @16:10:20.33 SS.reconfigure SSexhale @16:10:20.39 SS.seek 0ml @16:10:29.18 Exhaust.select :close @16:10:29.30 SSV.dial CBV @16:10:29.33 CBV.dial SSV @16:10:30.17 Allowing 130s for 25.0ml of remaining 1475.0ml @16:10:30.18 Intake.select :open @16:10:32.37 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:10:32.39 SS.reconfigure SSinhale.with maxSpeed: 59 @16:10:32.44 SS.seek 25ml @16:10:40.23 Vacuum too high -- waiting up to 40s @16:10:41.18 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:10:41.20 SS.reconfigure SSinhale.with maxSpeed: 53 @16:10:41.25 SS.seek 25ml @16:10:57.52 Vacuum too high -- waiting up to 40s @16:10:58.64 Intake @33.1psia, vacuum from 20.0 to 24.0psi @16:10:58.66 SS.reconfigure SSinhale.with maxSpeed: 48 @16:10:58.71 SS.seek 25ml @16:11:09.79 Stroke completed -- waiting for pressure to equalize ... @16:11:12.42 Intake.select :close @16:11:12.59 SSV.dial :exhaust @16:11:13.42 Exhaust.select :open @16:11:13.54 SS.reconfigure SSexhale @16:11:13.60 SS.seek 0ml @16:11:22.80 Exhaust.select :close @16:11:22.91 SSV.dial CBV @16:11:22.94 CBV.dial SSV @16:11:23.77 Allowing 130s for 25.0ml of remaining 1450.0ml @16:11:23.78 Intake.select :open @16:11:25.97 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:11:25.99 SS.reconfigure SSinhale.with maxSpeed: 48 @16:11:26.04 SS.seek 25ml @16:12:03.09 Stroke completed -- waiting for pressure to equalize ... @16:12:05.70 Intake.select :close @16:12:05.87 SSV.dial :exhaust @16:12:06.69 Exhaust.select :open @16:12:06.80 SS.reconfigure SSexhale @16:12:06.86 SS.seek 0ml @16:12:15.75 delay 5 seconds @16:12:15.91 Exhaust.select :close @16:12:16.03 SSV.dial CBV @16:12:16.06 CBV.dial SSV @16:12:16.89 Allowing 130s for 25.0ml of remaining 1425.0ml @16:12:16.90 Intake.select :open @16:12:19.09 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:12:19.11 SS.reconfigure SSinhale.with maxSpeed: 48 @16:12:19.16 SS.seek 25ml @16:12:20.76 ATV.dial ASV @16:12:21.72 ASV.dial :SPEdelivery @16:12:21.74 AS.seek 0.02ml @16:12:47.64 Vacuum too high -- waiting up to 40s @16:12:48.50 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:12:48.52 SS.reconfigure SSinhale.with maxSpeed: 43 @16:12:48.57 SS.seek 25ml @16:12:58.51 Stroke completed -- waiting for pressure to equalize ... @16:13:01.12 Intake.select :close @16:13:01.28 SSV.dial :exhaust @16:13:02.11 Exhaust.select :open @16:13:02.23 SS.reconfigure SSexhale @16:13:02.29 SS.seek 0ml @16:13:11.37 Exhaust.select :close @16:13:11.49 SSV.dial CBV @16:13:11.52 CBV.dial SSV @16:13:12.36 Allowing 130s for 25.0ml of remaining 1400.0ml @16:13:12.37 Intake.select :open @16:13:15.14 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:13:15.16 SS.reconfigure SSinhale.with maxSpeed: 43 @16:13:15.21 SS.seek 25ml @16:13:56.57 Stroke completed -- waiting for pressure to equalize ... @16:13:59.19 Intake.select :close @16:13:59.36 SSV.dial :exhaust @16:14:00.19 Exhaust.select :open @16:14:00.30 SS.reconfigure SSexhale @16:14:00.36 SS.seek 0ml @16:14:09.38 Exhaust.select :close @16:14:09.50 SSV.dial CBV @16:14:09.53 CBV.dial SSV @16:14:10.38 Allowing 130s for 25.0ml of remaining 1375.0ml @16:14:10.39 Intake.select :open @16:14:12.57 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:14:12.59 SS.reconfigure SSinhale.with maxSpeed: 43 @16:14:12.64 SS.seek 25ml @16:14:54.09 Stroke completed -- waiting for pressure to equalize ... @16:14:56.86 Intake.select :close @16:14:57.03 SSV.dial :exhaust @16:14:57.86 Exhaust.select :open @16:14:57.97 SS.reconfigure SSexhale @16:14:58.03 SS.seek 0ml @16:15:07.15 Exhaust.select :close @16:15:07.27 SSV.dial CBV @16:15:07.30 CBV.dial SSV @16:15:08.13 Allowing 130s for 25.0ml of remaining 1350.0ml @16:15:08.14 Intake.select :open @16:15:10.33 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:15:10.35 SS.reconfigure SSinhale.with maxSpeed: 43 @16:15:10.40 SS.seek 25ml @16:15:39.09 delay 5 seconds @16:15:44.11 ATV.dial :air2 @16:15:45.23 AS.seek 0.52ml @16:15:51.41 Stroke completed -- waiting for pressure to equalize ... @16:15:54.02 Intake.select :close @16:15:54.18 SSV.dial :exhaust @16:15:55.00 Exhaust.select :open @16:15:55.11 SS.reconfigure SSexhale @16:15:55.17 SS.seek 0ml @16:16:03.88 Exhaust.select :close @16:16:04.00 SSV.dial CBV @16:16:04.03 CBV.dial SSV @16:16:04.86 Allowing 130s for 25.0ml of remaining 1325.0ml @16:16:04.87 Intake.select :open @16:16:07.06 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:16:07.08 SS.reconfigure SSinhale.with maxSpeed: 43 @16:16:07.13 SS.seek 25ml @16:16:48.82 Stroke completed -- waiting for pressure to equalize ... @16:16:52.06 Intake.select :close @16:16:52.22 SSV.dial :exhaust @16:16:53.05 Exhaust.select :open @16:16:53.16 SS.reconfigure SSexhale @16:16:53.22 SS.seek 0ml @16:17:02.31 Exhaust.select :close @16:17:02.43 SSV.dial CBV @16:17:02.46 CBV.dial SSV @16:17:03.86 Allowing 130s for 25.0ml of remaining 1300.0ml @16:17:03.87 Intake.select :open @16:17:06.05 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:17:06.07 SS.reconfigure SSinhale.with maxSpeed: 43 @16:17:06.12 SS.seek 25ml @16:17:26.14 delay 5 seconds @16:17:31.16 ATV.dial ASV @16:17:32.11 ASV.dial :wasteR @16:17:32.48 AS.seek 0.3ml @16:17:47.57 Stroke completed -- waiting for pressure to equalize ... @16:17:50.81 Intake.select :close @16:17:50.98 SSV.dial :exhaust @16:17:51.82 Exhaust.select :open @16:17:51.93 SS.reconfigure SSexhale @16:17:51.99 SS.seek 0ml @16:18:00.76 Exhaust.select :close @16:18:00.88 SSV.dial CBV @16:18:00.91 CBV.dial SSV @16:18:01.76 Allowing 130s for 25.0ml of remaining 1275.0ml @16:18:01.78 Intake.select :open @16:18:03.95 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:18:03.97 SS.reconfigure SSinhale.with maxSpeed: 43 @16:18:04.02 SS.seek 25ml @16:18:17.00 delay 20 seconds @16:18:37.03 AS.seek 0.07ml @16:18:45.76 Stroke completed -- waiting for pressure to equalize ... @16:18:49.02 Intake.select :close @16:18:49.19 SSV.dial :exhaust @16:18:50.02 Exhaust.select :open @16:18:50.13 SS.reconfigure SSexhale @16:18:50.18 SS.seek 0ml @16:18:59.21 Exhaust.select :close @16:18:59.33 SSV.dial CBV @16:18:59.36 CBV.dial SSV @16:19:00.19 Allowing 130s for 25.0ml of remaining 1250.0ml @16:19:00.20 Intake.select :open @16:19:02.39 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:19:02.41 SS.reconfigure SSinhale.with maxSpeed: 43 @16:19:02.46 SS.seek 25ml @16:19:23.55 delay 20 seconds @16:19:43.57 ATV.dial :waste2 @16:19:43.61 Stroke completed -- waiting for pressure to equalize ... @16:19:44.45 AS.reconfigure ASconfig @16:19:44.50 AS.seek :empty @16:19:46.88 Intake.select :close @16:19:47.05 SSV.dial :exhaust @16:19:47.88 Exhaust.select :open @16:19:47.99 SS.reconfigure SSexhale @16:19:48.05 SS.seek 0ml @16:19:48.89 Elution @16:19:48.90 ATV.dial :air1 @16:19:49.98 AS.reconfigure ASslow1 @16:19:50.03 AS.seek 0.049ml @16:19:56.87 Exhaust.select :close @16:19:56.99 SSV.dial CBV @16:19:57.02 CBV.dial SSV @16:19:57.86 Allowing 130s for 25.0ml of remaining 1225.0ml @16:19:57.87 Intake.select :open @16:20:00.06 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:20:00.08 SS.reconfigure SSinhale.with maxSpeed: 43 @16:20:00.13 SS.seek 25ml @16:20:00.45 delay 3 seconds @16:20:03.46 ATV.dial :ElutionWater @16:20:04.21 AS.seek 0.109ml @16:20:16.56 delay 3 seconds @16:20:19.59 ATV.dial ASV,via: :air2 @16:20:21.55 ASV.dial :SPEelution @16:20:22.11 AFV.select :carrierSolution @16:20:22.28 AS.reconfigure ASnorm @16:20:22.33 AS.seek 0.759ml @16:20:41.51 Stroke completed -- waiting for pressure to equalize ... @16:20:45.38 Intake.select :close @16:20:45.54 SSV.dial :exhaust @16:20:46.36 Exhaust.select :open @16:20:46.47 SS.reconfigure SSexhale @16:20:46.54 SS.seek 0ml @16:20:55.38 delay 3 seconds @16:20:55.60 Exhaust.select :close @16:20:55.72 SSV.dial CBV @16:20:55.75 CBV.dial SSV @16:20:56.58 Allowing 130s for 25.0ml of remaining 1200.0ml @16:20:56.59 Intake.select :open @16:20:58.40 AFV.select :holdingCoil @16:20:58.56 AS.reconfigure ASslow1 @16:20:58.62 AS.seek 0.474ml @16:20:58.78 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:20:58.80 SS.reconfigure SSinhale.with maxSpeed: 43 @16:20:58.85 SS.seek 25ml @16:21:34.45 Vacuum too high -- waiting up to 40s @16:21:35.57 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:21:35.59 SS.reconfigure SSinhale.with maxSpeed: 39 @16:21:35.65 SS.seek 25ml @16:21:41.85 Stroke completed -- waiting for pressure to equalize ... @16:21:45.10 Intake.select :close @16:21:45.26 SSV.dial :exhaust @16:21:46.10 Exhaust.select :open @16:21:46.22 SS.reconfigure SSexhale @16:21:46.28 SS.seek 0ml @16:21:55.66 Exhaust.select :close @16:21:55.78 SSV.dial CBV @16:21:55.81 CBV.dial SSV @16:21:56.20 delay 5 seconds @16:21:56.64 Allowing 130s for 25.0ml of remaining 1175.0ml @16:21:56.65 Intake.select :open @16:21:58.83 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:21:58.85 SS.reconfigure SSinhale.with maxSpeed: 39 @16:21:58.90 SS.seek 25ml @16:22:01.29 AS.reconfigure ASslow2 @16:22:01.34 AS.seek 0.439ml @16:22:36.74 delay 1:00 @16:22:44.16 Stroke completed -- waiting for pressure to equalize ... @16:22:47.41 Intake.select :close @16:22:47.57 SSV.dial :exhaust @16:22:48.39 Exhaust.select :open @16:22:48.50 SS.reconfigure SSexhale @16:22:48.55 SS.seek 0ml @16:22:58.34 Exhaust.select :close @16:22:58.46 SSV.dial CBV @16:22:58.49 CBV.dial SSV @16:22:59.32 Allowing 130s for 25.0ml of remaining 1150.0ml @16:22:59.33 Intake.select :open @16:23:01.51 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:23:01.53 SS.reconfigure SSinhale.with maxSpeed: 39 @16:23:01.59 SS.seek 25ml @16:23:36.77 pushing eluate to holding coil @16:23:36.84 AS.seek 0.279ml @16:23:46.87 Stroke completed -- waiting for pressure to equalize ... @16:23:50.77 Intake.select :close @16:23:50.94 SSV.dial :exhaust @16:23:51.77 Exhaust.select :open @16:23:51.89 SS.reconfigure SSexhale @16:23:51.95 SS.seek 0ml @16:24:01.10 Exhaust.select :close @16:24:01.22 SSV.dial CBV @16:24:01.25 CBV.dial SSV @16:24:02.10 Allowing 130s for 25.0ml of remaining 1125.0ml @16:24:02.11 Intake.select :open @16:24:04.30 Intake @33.5psia, vacuum from 20.0 to 24.0psi @16:24:04.32 SS.reconfigure SSinhale.with maxSpeed: 39 @16:24:04.37 SS.seek 25ml @16:24:49.85 Stroke completed -- waiting for pressure to equalize ... @16:24:53.73 Intake.select :close @16:24:53.89 SSV.dial :exhaust @16:24:54.72 Exhaust.select :open @16:24:54.83 SS.reconfigure SSexhale @16:24:54.90 SS.seek 0ml @16:25:04.12 Exhaust.select :close @16:25:04.24 SSV.dial CBV @16:25:04.27 CBV.dial SSV @16:25:05.10 Allowing 130s for 25.0ml of remaining 1100.0ml @16:25:05.11 Intake.select :open @16:25:07.30 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:25:07.32 SS.reconfigure SSinhale.with maxSpeed: 39 @16:25:07.37 SS.seek 25ml @16:25:52.95 Stroke completed -- waiting for pressure to equalize ... @16:25:57.45 Intake.select :close @16:25:57.61 SSV.dial :exhaust @16:25:58.45 Exhaust.select :open @16:25:58.56 SS.reconfigure SSexhale @16:25:58.61 SS.seek 0ml @16:26:07.71 Exhaust.select :close @16:26:07.83 SSV.dial CBV @16:26:07.86 CBV.dial SSV @16:26:08.71 Allowing 130s for 25.0ml of remaining 1075.0ml @16:26:08.72 Intake.select :open @16:26:10.91 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:26:10.93 SS.reconfigure SSinhale.with maxSpeed: 39 @16:26:10.98 SS.seek 25ml @16:26:17.88 delay 30 seconds @16:26:47.91 ATV.dial :waste,via: :air3 @16:26:50.09 AS.reconfigure ASconfig @16:26:50.23 AS.seek :empty @16:26:56.24 Stroke completed -- waiting for pressure to equalize ... @16:27:00.77 Intake.select :close @16:27:00.94 SSV.dial :exhaust @16:27:01.78 Exhaust.select :open @16:27:01.90 SS.reconfigure SSexhale @16:27:01.96 SS.seek 0ml @16:27:06.63 Parking Bleach in LysateSource line and SPEcolumn @16:27:06.64 ATV.dial :air3 @16:27:07.53 AS.reconfigure ASslow1 @16:27:07.58 AS.seek 0.029ml @16:27:10.76 Exhaust.select :close @16:27:10.88 SSV.dial CBV @16:27:10.91 CBV.dial SSV @16:27:11.75 Allowing 130s for 25.0ml of remaining 1050.0ml @16:27:11.76 Intake.select :open @16:27:13.96 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:27:13.98 ATV.dial :ElutionWater @16:27:14.01 SS.reconfigure SSinhale.with maxSpeed: 39 @16:27:14.11 SS.seek 25ml @16:27:14.62 AS.seek 0.059ml @16:27:21.02 ATV.dial :air3 @16:27:21.62 AS.seek 0.069ml @16:27:23.89 delay 2 seconds @16:27:25.90 ATV.dial :bleach @16:27:26.36 AS.seek 0.769ml @16:27:51.36 Vacuum too high -- waiting up to 40s @16:27:52.49 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:27:52.51 SS.reconfigure SSinhale.with maxSpeed: 35 @16:27:52.56 SS.seek 25ml @16:28:02.34 Stroke completed -- waiting for pressure to equalize ... @16:28:06.20 Intake.select :close @16:28:06.37 SSV.dial :exhaust @16:28:07.17 Exhaust.select :open @16:28:07.28 SS.reconfigure SSexhale @16:28:07.34 SS.seek 0ml @16:28:16.48 Exhaust.select :close @16:28:16.60 SSV.dial CBV @16:28:16.63 CBV.dial SSV @16:28:17.47 Allowing 130s for 25.0ml of remaining 1025.0ml @16:28:17.48 Intake.select :open @16:28:19.67 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:28:19.69 SS.reconfigure SSinhale.with maxSpeed: 35 @16:28:19.74 SS.seek 25ml @16:29:10.05 Stroke completed -- waiting for pressure to equalize ... @16:29:13.92 Intake.select :close @16:29:14.69 SSV.dial :exhaust @16:29:15.53 Exhaust.select :open @16:29:15.65 SS.reconfigure SSexhale @16:29:15.71 SS.seek 0ml @16:29:24.73 Exhaust.select :close @16:29:24.85 SSV.dial CBV @16:29:24.88 CBV.dial SSV @16:29:25.72 Allowing 130s for 25.0ml of remaining 1000.0ml @16:29:25.73 Intake.select :open @16:29:27.91 Intake @35.0psia, vacuum from 20.0 to 24.0psi @16:29:27.93 SS.reconfigure SSinhale.with maxSpeed: 35 @16:29:27.98 SS.seek 25ml @16:29:47.39 delay 3 seconds @16:29:50.41 ATV.dial :air3 @16:29:50.94 delay 3 seconds @16:29:54.03 AS.seek 0.794ml @16:29:59.39 delay 3 seconds @16:30:02.41 ATV.dial :bleach @16:30:03.01 AS.seek 0.779ml @16:30:06.28 delay 3 seconds @16:30:09.30 ATV.dial :AIV @16:30:10.49 AS.reconfigure ASconfig @16:30:10.55 AS.seek 0.015ml @16:30:18.73 Stroke completed -- waiting for pressure to equalize ... @16:30:23.26 Intake.select :close @16:30:23.43 SSV.dial :exhaust @16:30:24.25 Exhaust.select :open @16:30:24.36 SS.reconfigure SSexhale @16:30:24.42 SS.seek 0ml @16:30:33.68 Exhaust.select :close @16:30:33.80 SSV.dial CBV @16:30:33.83 CBV.dial SSV @16:30:34.66 Allowing 130s for 25.0ml of remaining 975.0ml @16:30:34.67 Intake.select :open @16:30:36.85 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:30:36.87 SS.reconfigure SSinhale.with maxSpeed: 35 @16:30:36.92 SS.seek 25ml @16:30:54.73 ATV.dial :waste @16:30:56.24 AS.seek :empty @16:30:57.45 ATV.dial :air3 @16:30:58.12 AS.reconfigure ASslow1 @16:30:58.17 AS.seek 0.009ml @16:31:00.57 delay 2 seconds @16:31:02.59 ATV.dial :bleach @16:31:03.04 AS.seek 0.579ml @16:31:27.46 Stroke completed -- waiting for pressure to equalize ... @16:31:31.97 Intake.select :close @16:31:32.13 SSV.dial :exhaust @16:31:32.97 Exhaust.select :open @16:31:33.08 SS.reconfigure SSexhale @16:31:33.14 SS.seek 0ml @16:31:42.48 Exhaust.select :close @16:31:42.60 SSV.dial CBV @16:31:42.63 CBV.dial SSV @16:31:43.47 Allowing 130s for 25.0ml of remaining 950.0ml @16:31:43.48 Intake.select :open @16:31:45.66 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:31:45.68 SS.reconfigure SSinhale.with maxSpeed: 35 @16:31:45.73 SS.seek 25ml @16:32:36.37 Stroke completed -- waiting for pressure to equalize ... @16:32:40.21 Intake.select :close @16:32:40.38 SSV.dial :exhaust @16:32:41.21 Exhaust.select :open @16:32:41.32 SS.reconfigure SSexhale @16:32:41.38 SS.seek 0ml @16:32:50.77 Exhaust.select :close @16:32:50.89 SSV.dial CBV @16:32:50.92 CBV.dial SSV @16:32:51.74 Allowing 130s for 25.0ml of remaining 925.0ml @16:32:51.75 Intake.select :open @16:32:53.94 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:32:53.96 SS.reconfigure SSinhale.with maxSpeed: 35 @16:32:54.01 SS.seek 25ml @16:32:57.93 delay 3 seconds @16:33:00.95 ATV.dial :air3 @16:33:01.49 delay 3 seconds @16:33:04.58 AS.seek 0.604ml @16:33:09.87 delay 3 seconds @16:33:12.89 ATV.dial :bleach @16:33:13.50 AS.seek 0.589ml @16:33:16.80 delay 3 seconds @16:33:19.82 ATV.dial :ASV @16:33:20.80 ASV.dial :wasteR @16:33:21.37 AS.seek 0ml @16:33:44.16 Stroke completed -- waiting for pressure to equalize ... @16:33:49.33 Intake.select :close @16:33:49.50 SSV.dial :exhaust @16:33:50.33 Exhaust.select :open @16:33:50.44 SS.reconfigure SSexhale @16:33:50.50 SS.seek 0ml @16:33:59.41 Exhaust.select :close @16:33:59.53 SSV.dial CBV @16:33:59.56 CBV.dial SSV @16:34:00.39 Allowing 130s for 25.0ml of remaining 900.0ml @16:34:00.40 Intake.select :open @16:34:02.59 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:34:02.61 SS.reconfigure SSinhale.with maxSpeed: 35 @16:34:02.66 SS.seek 25ml @16:34:53.48 Stroke completed -- waiting for pressure to equalize ... @16:34:58.62 Intake.select :close @16:34:58.78 SSV.dial :exhaust @16:34:59.61 Exhaust.select :open @16:34:59.72 SS.reconfigure SSexhale @16:34:59.78 SS.seek 0ml @16:35:08.66 Exhaust.select :close @16:35:08.78 SSV.dial CBV @16:35:08.81 CBV.dial SSV @16:35:09.64 Allowing 130s for 25.0ml of remaining 875.0ml @16:35:09.65 Intake.select :open @16:35:11.84 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:35:11.86 SS.reconfigure SSinhale.with maxSpeed: 35 @16:35:11.91 SS.seek 25ml @16:35:20.18 delay 1:00 @16:36:03.43 Stroke completed -- waiting for pressure to equalize ... @16:36:09.27 Intake.select :close @16:36:09.44 SSV.dial :exhaust @16:36:10.27 Exhaust.select :open @16:36:10.39 SS.reconfigure SSexhale @16:36:10.45 SS.seek 0ml @16:36:19.52 Exhaust.select :close @16:36:19.64 SSV.dial CBV @16:36:19.67 CBV.dial SSV @16:36:20.19 AFV.select :carrierSolution @16:36:20.30 delay 2 seconds @16:36:20.50 Allowing 130s for 25.0ml of remaining 850.0ml @16:36:20.51 Intake.select :open @16:36:22.33 AS.reconfigure ASconfig @16:36:22.38 AS.seek 1ml @16:36:22.70 Intake @33.3psia, vacuum from 20.0 to 24.0psi @16:36:22.72 SS.reconfigure SSinhale.with maxSpeed: 35 @16:36:22.77 SS.seek 25ml @16:37:13.18 Stroke completed -- waiting for pressure to equalize ... @16:37:19.63 Intake.select :close @16:37:19.79 SSV.dial :exhaust @16:37:20.15 delay 1 second @16:37:20.61 Exhaust.select :open @16:37:20.73 SS.reconfigure SSexhale @16:37:20.79 SS.seek 0ml @16:37:21.16 AFV.select :holdingCoil @16:37:21.27 delay 1 second @16:37:22.29 ATV.dial ASV @16:37:22.31 ASV.dial :wasteR @16:37:22.33 AS.reconfigure ASslow1 @16:37:22.37 AS.seek 0ml @16:37:29.71 Exhaust.select :close @16:37:29.83 SSV.dial CBV @16:37:29.86 CBV.dial SSV @16:37:30.71 Allowing 130s for 25.0ml of remaining 825.0ml @16:37:30.72 Intake.select :open @16:37:32.91 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:37:32.93 SS.reconfigure SSinhale.with maxSpeed: 35 @16:37:32.98 SS.seek 25ml @16:37:57.04 Vacuum too high -- waiting up to 40s @16:37:58.42 Intake @33.5psia, vacuum from 20.0 to 24.0psi @16:37:58.44 SS.reconfigure SSinhale.with maxSpeed: 32 @16:37:58.50 SS.seek 25ml @16:38:27.90 Stroke completed -- waiting for pressure to equalize ... @16:38:33.65 Intake.select :close @16:38:33.81 SSV.dial :exhaust @16:38:34.64 Exhaust.select :open @16:38:34.76 SS.reconfigure SSexhale @16:38:34.82 SS.seek 0ml @16:38:43.96 Exhaust.select :close @16:38:44.07 SSV.dial CBV @16:38:44.10 CBV.dial SSV @16:38:44.92 Allowing 130s for 25.0ml of remaining 800.0ml @16:38:44.93 Intake.select :open @16:38:47.13 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:38:47.15 SS.reconfigure SSinhale.with maxSpeed: 32 @16:38:47.20 SS.seek 25ml @16:39:41.99 Stroke completed -- waiting for pressure to equalize ... @16:39:48.44 Intake.select :close @16:39:48.60 SSV.dial :exhaust @16:39:49.42 Exhaust.select :open @16:39:49.53 SS.reconfigure SSexhale @16:39:49.59 SS.seek 0ml @16:39:58.91 Exhaust.select :close @16:39:59.03 SSV.dial CBV @16:39:59.06 CBV.dial SSV @16:39:59.89 Allowing 130s for 25.0ml of remaining 775.0ml @16:39:59.90 Intake.select :open @16:40:02.09 Intake @35.1psia, vacuum from 20.0 to 24.0psi @16:40:02.11 SS.reconfigure SSinhale.with maxSpeed: 32 @16:40:02.16 SS.seek 25ml @16:40:30.14 Vacuum too high -- waiting up to 40s @16:40:31.51 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:40:31.53 SS.reconfigure SSinhale.with maxSpeed: 29 @16:40:31.58 SS.seek 25ml @16:40:43.76 delay 20 seconds @16:41:01.23 Stroke completed -- waiting for pressure to equalize ... @16:41:03.79 ATV.dial :air3 @16:41:05.22 AS.seek 0.59ml @16:41:06.39 Intake.select :close @16:41:06.56 SSV.dial :exhaust @16:41:07.39 Exhaust.select :open @16:41:07.50 SS.reconfigure SSexhale @16:41:07.56 SS.seek 0ml @16:41:16.71 Exhaust.select :close @16:41:16.83 SSV.dial CBV @16:41:16.86 CBV.dial SSV @16:41:18.28 Allowing 130s for 25.0ml of remaining 750.0ml @16:41:18.29 Intake.select :open @16:41:20.48 Intake @33.8psia, vacuum from 20.0 to 24.0psi @16:41:20.50 SS.reconfigure SSinhale.with maxSpeed: 29 @16:41:20.55 SS.seek 25ml @16:42:20.99 Stroke completed -- waiting for pressure to equalize ... @16:42:26.80 Intake.select :close @16:42:26.96 SSV.dial :exhaust @16:42:27.78 Exhaust.select :open @16:42:27.90 SS.reconfigure SSexhale @16:42:27.96 SS.seek 0ml @16:42:37.10 Exhaust.select :close @16:42:37.22 SSV.dial CBV @16:42:37.25 CBV.dial SSV @16:42:38.10 Allowing 130s for 25.0ml of remaining 725.0ml @16:42:38.11 Intake.select :open @16:42:40.29 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:42:40.31 SS.reconfigure SSinhale.with maxSpeed: 29 @16:42:40.36 SS.seek 25ml @16:43:04.13 delay 3 seconds @16:43:07.15 ATV.dial :ASV @16:43:08.59 ASV.dial :wasteR @16:43:08.68 AS.seek 0.09ml @16:43:40.98 Stroke completed -- waiting for pressure to equalize ... @16:43:47.38 Intake.select :close @16:43:47.54 SSV.dial :exhaust @16:43:48.36 Exhaust.select :open @16:43:48.47 SS.reconfigure SSexhale @16:43:48.53 SS.seek 0ml @16:43:57.38 Exhaust.select :close @16:43:57.50 SSV.dial CBV @16:43:57.53 CBV.dial SSV @16:43:58.36 Allowing 130s for 25.0ml of remaining 700.0ml @16:43:58.37 Intake.select :open @16:44:00.56 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:44:00.58 SS.reconfigure SSinhale.with maxSpeed: 29 @16:44:00.63 SS.seek 25ml @16:44:49.52 delay 30 seconds @16:45:01.54 Stroke completed -- waiting for pressure to equalize ... @16:45:08.60 Intake.select :close @16:45:09.37 SSV.dial :exhaust @16:45:10.19 Exhaust.select :open @16:45:10.30 SS.reconfigure SSexhale @16:45:10.36 SS.seek 0ml @16:45:19.54 ATV.dial :extSrc @16:45:19.68 Exhaust.select :close @16:45:19.80 SSV.dial CBV @16:45:19.83 CBV.dial SSV @16:45:20.26 delay 3 seconds @16:45:20.66 Allowing 130s for 25.0ml of remaining 675.0ml @16:45:20.67 Intake.select :open @16:45:22.86 Intake @35.0psia, vacuum from 20.0 to 24.0psi @16:45:22.88 SS.reconfigure SSinhale.with maxSpeed: 29 @16:45:22.93 SS.seek 25ml @16:45:23.28 ATV.dial :ElutionWater @16:45:24.36 AS.seek 0.12ml @16:45:30.70 ATV.dial :air3 @16:45:31.30 AS.seek 0.13ml @16:45:33.60 delay 2 seconds @16:45:35.61 ATV.dial :bleach @16:45:36.08 AS.seek 0.23ml @16:45:56.54 delay 3 seconds @16:45:56.79 Vacuum too high -- waiting up to 40s @16:45:58.43 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:45:58.45 SS.reconfigure SSinhale.with maxSpeed: 26 @16:45:58.50 SS.seek 25ml @16:45:59.55 ATV.dial :air3 @16:46:00.09 delay 3 seconds @16:46:03.18 AS.seek 0.255ml @16:46:08.52 delay 3 seconds @16:46:11.54 ATV.dial :bleach @16:46:12.14 AS.seek 0.24ml @16:46:15.41 delay 3 seconds @16:46:18.43 ATV.dial :ASV @16:46:19.41 ASV.dial :SPEdelivery @16:46:19.77 AFV.select :carrierSolution @16:46:19.88 delay 2 seconds @16:46:21.91 AS.reconfigure ASconfig @16:46:21.96 AS.seek 0.5ml @16:46:28.45 Stroke completed -- waiting for pressure to equalize ... @16:46:34.22 Intake.select :close @16:46:34.38 SSV.dial :exhaust @16:46:35.21 Exhaust.select :open @16:46:35.32 SS.reconfigure SSexhale @16:46:35.38 SS.seek 0ml @16:46:37.21 delay 1 second @16:46:38.22 AFV.select :holdingCoil @16:46:38.38 AS.reconfigure ASslow1 @16:46:38.44 AS.seek 0.17ml @16:46:45.04 Exhaust.select :close @16:46:45.16 SSV.dial CBV @16:46:45.19 CBV.dial SSV @16:46:46.02 Allowing 130s for 25.0ml of remaining 650.0ml @16:46:46.03 Intake.select :open @16:46:48.22 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:46:48.24 SS.reconfigure SSinhale.with maxSpeed: 26 @16:46:48.29 SS.seek 25ml @16:47:45.11 SPE.hold 2:46:40,95C @16:47:45.18 delay 3:00 @16:47:55.76 Stroke completed -- waiting for pressure to equalize ... @16:48:02.16 Intake.select :close @16:48:02.32 SSV.dial :exhaust @16:48:03.14 Exhaust.select :open @16:48:03.26 SS.reconfigure SSexhale @16:48:03.32 SS.seek 0ml @16:48:12.50 Exhaust.select :close @16:48:12.62 SSV.dial CBV @16:48:12.65 CBV.dial SSV @16:48:13.50 Allowing 130s for 25.0ml of remaining 625.0ml @16:48:13.51 Intake.select :open @16:48:15.70 Intake @33.7psia, vacuum from 20.0 to 24.0psi @16:48:15.72 SS.reconfigure SSinhale.with maxSpeed: 26 @16:48:15.77 SS.seek 25ml @16:49:23.15 Stroke completed -- waiting for pressure to equalize ... @16:49:30.22 Intake.select :close @16:49:30.39 SSV.dial :exhaust @16:49:31.24 Exhaust.select :open @16:49:31.35 SS.reconfigure SSexhale @16:49:31.41 SS.seek 0ml @16:49:40.65 Exhaust.select :close @16:49:40.77 SSV.dial CBV @16:49:40.80 CBV.dial SSV @16:49:41.67 Allowing 130s for 25.0ml of remaining 600.0ml @16:49:41.68 Intake.select :open @16:49:43.86 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:49:43.88 SS.reconfigure SSinhale.with maxSpeed: 26 @16:49:43.93 SS.seek 25ml @16:50:45.21 AFV.select :carrierSolution @16:50:45.32 delay 2 seconds @16:50:47.35 AS.reconfigure ASconfig @16:50:47.40 AS.seek 1ml @16:50:51.23 Stroke completed -- waiting for pressure to equalize ... @16:50:57.66 Intake.select :close @16:50:57.82 SSV.dial :exhaust @16:50:58.66 Exhaust.select :open @16:50:58.77 SS.reconfigure SSexhale @16:50:58.83 SS.seek 0ml @16:51:07.98 Exhaust.select :close @16:51:08.10 SSV.dial CBV @16:51:08.13 CBV.dial SSV @16:51:09.61 Allowing 130s for 25.0ml of remaining 575.0ml @16:51:09.62 Intake.select :open @16:51:11.80 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:51:11.82 SS.reconfigure SSinhale.with maxSpeed: 26 @16:51:11.88 SS.seek 25ml @16:51:35.42 delay 1 second @16:51:36.45 AFV.select :holdingCoil @16:51:36.56 delay 1 second @16:51:37.58 ASV.dial :wasteD @16:51:38.60 AS.seek 0ml @16:51:55.59 Vacuum too high -- waiting up to 40s @16:51:57.23 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:51:57.25 SS.reconfigure SSinhale.with maxSpeed: 23 @16:51:57.30 SS.seek 25ml @16:52:24.30 Stroke completed -- waiting for pressure to equalize ... @16:52:30.76 Intake.select :close @16:52:30.93 SSV.dial :exhaust @16:52:31.75 Exhaust.select :open @16:52:31.86 SS.reconfigure SSexhale @16:52:31.92 SS.seek 0ml @16:52:36.40 Priming Eluate @16:52:36.41 AFV.select :carrierSolution @16:52:36.52 delay 2 seconds @16:52:38.55 AS.seek 0.5ml @16:52:40.96 Exhaust.select :close @16:52:41.07 SSV.dial CBV @16:52:41.10 CBV.dial SSV @16:52:41.96 Allowing 130s for 25.0ml of remaining 550.0ml @16:52:41.97 Intake.select :open @16:52:44.15 Intake @35.1psia, vacuum from 20.0 to 24.0psi @16:52:44.17 SS.reconfigure SSinhale.with maxSpeed: 23 @16:52:44.22 SS.seek 25ml @16:53:07.62 delay 1 second @16:53:08.64 AFV.select :holdingCoil @16:53:08.75 ATV.dial ASV @16:53:08.76 ASV.dial :SPErecovery @16:53:09.34 Swish #1 of 2 @16:53:09.42 AS.reconfigure ASslow0 @16:53:09.47 AS.seek 0.6ml @16:53:19.92 AS.seek 0.5ml @16:53:30.28 Swish #2 of 2 @16:53:30.37 AS.seek 0.6ml @16:53:40.86 AS.seek 0.5ml @16:53:51.26 delay 3 seconds @16:53:54.35 AS.reconfigure ASslow1 @16:53:54.40 AS.seek 0.684ml @16:54:00.71 Stroke completed -- waiting for pressure to equalize ... @16:54:07.17 Intake.select :close @16:54:07.33 SSV.dial :exhaust @16:54:08.78 Exhaust.select :open @16:54:08.89 SS.reconfigure SSexhale @16:54:08.95 SS.seek 0ml @16:54:18.30 Exhaust.select :close @16:54:18.42 SSV.dial CBV @16:54:18.45 CBV.dial SSV @16:54:19.28 Allowing 130s for 25.0ml of remaining 525.0ml @16:54:19.29 Intake.select :open @16:54:21.47 Intake @35.0psia, vacuum from 20.0 to 24.0psi @16:54:21.49 SS.reconfigure SSinhale.with maxSpeed: 23 @16:54:21.54 SS.seek 25ml @16:54:31.81 delay 3 seconds @16:54:34.84 Eluate primed @16:54:34.85 ATV.dial :waste2 @16:54:35.43 AS.reconfigure ASnorm @16:54:35.49 AS.seek 0ml @16:55:10.28 SPE.coast @16:55:10.37 ASV.dial :SPErecovery @16:55:10.39 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct0916h1500ml.pcr @16:55:10.41 Building PCR Slug from ASV with MM2 mix, PP4 primer @16:55:10.42 Priming with carrier @16:55:10.43 ATV.dial ARV @16:55:11.35 ARV.dial :carrier @16:55:11.69 AS.seek 0.5ml @16:55:37.22 AFV.select :carrierSolution @16:55:37.34 delay 2 seconds @16:55:37.77 Stroke completed -- waiting for pressure to equalize ... @16:55:39.37 AS.reconfigure ASconfig @16:55:39.43 AS.seek 1ml @16:55:44.84 Intake.select :close @16:55:45.00 SSV.dial :exhaust @16:55:45.83 Exhaust.select :open @16:55:45.95 SS.reconfigure SSexhale @16:55:46.01 SS.seek 0ml @16:55:55.01 Exhaust.select :close @16:55:55.13 SSV.dial CBV @16:55:55.16 CBV.dial SSV @16:55:56.00 Allowing 130s for 25.0ml of remaining 500.0ml @16:55:56.01 Intake.select :open @16:55:58.20 Intake @35.3psia, vacuum from 20.0 to 24.0psi @16:55:58.22 SS.reconfigure SSinhale.with maxSpeed: 23 @16:55:58.27 SS.seek 25ml @16:56:08.45 delay 1 second @16:56:09.47 AFV.select :holdingCoil @16:56:09.58 delay 1 second @16:56:10.60 ATV.dial :waste @16:56:12.03 AS.reconfigure ASnorm @16:56:12.08 AS.seek :empty @16:57:00.80 Vacuum too high -- waiting up to 40s @16:57:02.42 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:57:02.45 SS.reconfigure SSinhale.with maxSpeed: 21 @16:57:02.50 SS.seek 25ml @16:57:02.83 AFV.select :carrierSolution @16:57:02.95 delay 2 seconds @16:57:04.98 AS.reconfigure ASconfig @16:57:05.03 AS.seek 0.5ml @16:57:17.88 Stroke completed -- waiting for pressure to equalize ... @16:57:25.03 Intake.select :close @16:57:25.21 SSV.dial :exhaust @16:57:26.03 Exhaust.select :open @16:57:26.15 SS.reconfigure SSexhale @16:57:26.21 SS.seek 0ml @16:57:34.14 delay 1 second @16:57:35.16 AFV.select :holdingCoil @16:57:35.27 Building Slug @16:57:35.28 ATV.dial :ARV @16:57:35.41 Exhaust.select :close @16:57:35.52 SSV.dial CBV @16:57:35.55 CBV.dial SSV @16:57:36.39 Allowing 130s for 25.0ml of remaining 475.0ml @16:57:36.40 Intake.select :open @16:57:36.89 delay 2 seconds @16:57:38.58 Intake @35.1psia, vacuum from 20.0 to 24.0psi @16:57:38.61 SS.reconfigure SSinhale.with maxSpeed: 21 @16:57:38.66 SS.seek 25ml @16:57:38.91 ARV.dial :air1 @16:57:39.41 delay 2 seconds @16:57:41.50 AS.reconfigure ASslow2 @16:57:41.55 AS.seek 0.506ml @16:57:48.01 delay 2 seconds @16:57:50.03 ARV.dial :MM2 @16:57:51.43 delay 2 seconds @16:57:53.46 Adding MM2 @16:57:53.54 AS.seek 0.524ml @16:58:11.86 delay 25 seconds @16:58:36.88 ARV.dial :PP4 @16:58:37.28 Adding PP4 @16:58:37.29 delay 2 seconds @16:58:39.39 AS.seek 0.53ml @16:58:45.78 delay 10 seconds @16:58:55.80 ARV.dial :air3 @16:58:56.20 adding ARV air @16:58:56.21 delay 2 seconds @16:58:58.30 AS.seek 0.536ml @16:59:02.07 Stroke completed -- waiting for pressure to equalize ... @16:59:04.66 delay 5 seconds @16:59:09.20 Intake.select :close @16:59:09.37 SSV.dial :exhaust @16:59:09.69 ARV.dial :carrier @16:59:10.19 Exhaust.select :open @16:59:10.31 SS.reconfigure SSexhale @16:59:10.37 SS.seek 0ml @16:59:10.87 delay 7 seconds @16:59:17.96 AS.seek 0.594ml @16:59:19.98 Exhaust.select :close @16:59:20.10 SSV.dial CBV @16:59:20.13 CBV.dial SSV @16:59:20.95 Allowing 130s for 25.0ml of remaining 450.0ml @16:59:20.96 Intake.select :open @16:59:23.15 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:59:23.17 SS.reconfigure SSinhale.with maxSpeed: 21 @16:59:23.22 SS.seek 25ml @17:00:16.54 delay 5 seconds @17:00:21.56 ATV.dial :ASV @17:00:21.97 delay 2 seconds @17:00:24.68 AS.seek 0.6ml @17:00:31.04 delay 4 seconds @17:00:35.06 ATV.dial ARV @17:00:35.59 delay 2 seconds @17:00:37.61 ARV.dial :carrier @17:00:37.63 delay 2 seconds @17:00:39.72 AS.seek 0.65ml @17:00:46.69 Stroke completed -- waiting for pressure to equalize ... @17:00:55.69 Intake.select :close @17:00:55.86 SSV.dial :exhaust @17:00:56.67 Exhaust.select :open @17:00:56.79 SS.reconfigure SSexhale @17:00:56.86 SS.seek 0ml @17:01:05.83 Exhaust.select :close @17:01:05.94 SSV.dial CBV @17:01:05.97 CBV.dial SSV @17:01:06.80 Allowing 130s for 25.0ml of remaining 425.0ml @17:01:06.81 Intake.select :open @17:01:08.99 Intake @35.4psia, vacuum from 20.0 to 24.0psi @17:01:09.01 SS.reconfigure SSinhale.with maxSpeed: 21 @17:01:09.06 SS.seek 25ml @17:01:30.18 delay 4 seconds @17:01:34.20 ATV.dial PCR @17:01:34.79 ASV.dial :waste1 @17:01:35.55 ARV.dial :air1 @17:01:35.92 Positioning Slug by 0.324ml @17:01:36.01 AS.seek 0.326ml @17:02:18.95 Vacuum too high -- waiting up to 40s @17:02:20.59 Intake @34.5psia, vacuum from 20.0 to 24.0psi @17:02:20.62 SS.reconfigure SSinhale.with maxSpeed: 19 @17:02:20.67 SS.seek 25ml @17:02:36.23 Stroke completed -- waiting for pressure to equalize ... @17:02:43.94 Intake.select :close @17:02:44.10 SSV.dial :exhaust @17:02:45.55 Exhaust.select :open @17:02:45.67 SS.reconfigure SSexhale @17:02:45.73 SS.seek 0ml @17:02:54.63 Exhaust.select :close @17:02:54.74 SSV.dial CBV @17:02:54.77 CBV.dial SSV @17:02:55.61 Allowing 130s for 25.0ml of remaining 400.0ml @17:02:55.62 Intake.select :open @17:02:57.80 Intake @35.3psia, vacuum from 20.0 to 24.0psi @17:02:57.82 SS.reconfigure SSinhale.with maxSpeed: 19 @17:02:57.88 SS.seek 25ml @17:04:29.97 Stroke completed -- waiting for pressure to equalize ... @17:04:37.69 Intake.select :close @17:04:37.86 SSV.dial :exhaust @17:04:38.69 Exhaust.select :open @17:04:38.80 SS.reconfigure SSexhale @17:04:38.86 SS.seek 0ml @17:04:48.19 Exhaust.select :close @17:04:48.31 SSV.dial CBV @17:04:48.34 CBV.dial SSV @17:04:49.16 Allowing 130s for 25.0ml of remaining 375.0ml @17:04:49.17 Intake.select :open @17:04:51.36 Intake @34.8psia, vacuum from 20.0 to 24.0psi @17:04:51.38 SS.reconfigure SSinhale.with maxSpeed: 19 @17:04:51.43 SS.seek 25ml @17:06:23.43 Stroke completed -- waiting for pressure to equalize ... @17:06:33.04 Intake.select :close @17:06:33.21 SSV.dial :exhaust @17:06:34.02 Exhaust.select :open @17:06:34.13 SS.reconfigure SSexhale @17:06:34.19 SS.seek 0ml @17:06:43.21 Exhaust.select :close @17:06:43.33 SSV.dial CBV @17:06:43.36 CBV.dial SSV @17:06:44.20 Allowing 130s for 25.0ml of remaining 350.0ml @17:06:44.21 Intake.select :open @17:06:46.40 Intake @34.9psia, vacuum from 20.0 to 24.0psi @17:06:46.42 SS.reconfigure SSinhale.with maxSpeed: 19 @17:06:46.47 SS.seek 25ml @17:07:02.26 delay 5 seconds @17:07:07.28 ATV.dial ARV @17:07:08.12 ASV.dial :PCRlock @17:07:09.12 delay 5 seconds @17:07:14.15 Running Tt1 Profile60C300 thermocycling @17:07:14.17 PCR.program Profile60C300 @17:07:14.30 Profile60C300 should complete in < 1:45:21 @17:07:14.31 PCR.run 2:16:41.25 @17:08:18.77 Stroke completed -- waiting for pressure to equalize ... @17:08:29.04 Intake.select :close @17:08:29.21 SSV.dial :exhaust @17:08:30.03 Exhaust.select :open @17:08:30.15 SS.reconfigure SSexhale @17:08:30.21 SS.seek 0ml @17:08:39.24 Exhaust.select :close @17:08:39.36 SSV.dial CBV @17:08:39.39 CBV.dial SSV @17:08:40.24 Allowing 130s for 25.0ml of remaining 325.0ml @17:08:40.25 Intake.select :open @17:08:42.43 Intake @34.9psia, vacuum from 20.0 to 24.0psi @17:08:42.45 SS.reconfigure SSinhale.with maxSpeed: 19 @17:08:42.50 SS.seek 25ml @17:10:04.07 Vacuum too high -- waiting up to 40s @17:10:06.49 Intake @35.2psia, vacuum from 20.0 to 24.0psi @17:10:06.51 SS.reconfigure SSinhale.with maxSpeed: 17 @17:10:06.56 SS.seek 25ml @17:10:19.07 Stroke completed -- waiting for pressure to equalize ... @17:10:27.46 Intake.select :close @17:10:27.62 SSV.dial :exhaust @17:10:28.45 Exhaust.select :open @17:10:28.57 SS.reconfigure SSexhale @17:10:28.63 SS.seek 0ml @17:10:37.71 Exhaust.select :close @17:10:37.83 SSV.dial CBV @17:10:37.86 CBV.dial SSV @17:10:38.69 Allowing 130s for 25.0ml of remaining 300.0ml @17:10:38.70 Intake.select :open @17:10:40.89 Intake @35.7psia, vacuum from 20.0 to 24.0psi @17:10:40.91 SS.reconfigure SSinhale.with maxSpeed: 17 @17:10:40.96 SS.seek 25ml @17:12:24.19 Stroke completed -- waiting for pressure to equalize ... @17:12:33.86 Intake.select :close @17:12:34.02 SSV.dial :exhaust @17:12:34.85 Exhaust.select :open @17:12:34.96 SS.reconfigure SSexhale @17:12:35.01 SS.seek 0ml @17:12:44.18 Exhaust.select :close @17:12:44.30 SSV.dial CBV @17:12:44.33 CBV.dial SSV @17:12:45.17 Allowing 130s for 25.0ml of remaining 275.0ml @17:12:45.18 Intake.select :open @17:12:47.37 Intake @35.5psia, vacuum from 20.0 to 24.0psi @17:12:47.39 SS.reconfigure SSinhale.with maxSpeed: 17 @17:12:47.44 SS.seek 25ml @17:14:30.35 Stroke completed -- waiting for pressure to equalize ... @17:14:41.27 Intake.select :close @17:14:41.43 SSV.dial :exhaust @17:14:42.25 Exhaust.select :open @17:14:42.37 SS.reconfigure SSexhale @17:14:42.42 SS.seek 0ml @17:14:51.79 Exhaust.select :close @17:14:51.91 SSV.dial CBV @17:14:51.94 CBV.dial SSV @17:14:52.77 Allowing 130s for 25.0ml of remaining 250.0ml @17:14:52.78 Intake.select :open @17:14:54.97 Intake @34.7psia, vacuum from 20.0 to 24.0psi @17:14:54.99 SS.reconfigure SSinhale.with maxSpeed: 17 @17:14:55.04 SS.seek 25ml @17:16:38.04 Stroke completed -- waiting for pressure to equalize ... @17:16:50.22 Intake.select :close @17:16:50.43 SSV.dial :exhaust @17:16:51.25 Exhaust.select :open @17:16:51.36 SS.reconfigure SSexhale @17:16:51.42 SS.seek 0ml @17:17:00.54 Exhaust.select :close @17:17:00.66 SSV.dial CBV @17:17:00.69 CBV.dial SSV @17:17:01.53 Allowing 130s for 25.0ml of remaining 225.0ml @17:17:01.54 Intake.select :open @17:17:03.73 Intake @35.8psia, vacuum from 20.0 to 24.0psi @17:17:03.75 SS.reconfigure SSinhale.with maxSpeed: 17 @17:17:03.80 SS.seek 25ml @17:17:40.40 Vacuum too high -- waiting up to 40s @17:17:42.42 Intake @35.2psia, vacuum from 20.0 to 24.0psi @17:17:42.44 SS.reconfigure SSinhale.with maxSpeed: 15 @17:17:42.50 SS.seek 25ml @17:18:58.55 Stroke completed -- waiting for pressure to equalize ... @17:19:08.81 Intake.select :close @17:19:08.98 SSV.dial :exhaust @17:19:09.81 Exhaust.select :open @17:19:09.92 SS.reconfigure SSexhale @17:19:09.98 SS.seek 0ml @17:19:19.41 Exhaust.select :close @17:19:19.53 SSV.dial CBV @17:19:19.56 CBV.dial SSV @17:19:20.41 Allowing 130s for 25.0ml of remaining 200.0ml @17:19:20.42 Intake.select :open @17:19:22.60 Intake @34.8psia, vacuum from 20.0 to 24.0psi @17:19:22.62 SS.reconfigure SSinhale.with maxSpeed: 15 @17:19:22.67 SS.seek 25ml @17:21:19.27 Stroke completed -- waiting for pressure to equalize ... @17:21:30.49 SS.reconfigure SSconfig @17:21:30.54 SS.hold @17:21:30.69 Intake.select :close @17:21:30.87 ShallowSampler::Clogged in WCR -- progress too slow after 1325.0ml @17:21:30.89 SSV.dial :exhaust @17:21:31.70 Exhaust.select :open @17:21:31.82 SS.reconfigure SSexhale @17:21:31.88 SS.seek 0ml @17:21:41.44 Exhaust.select :close @17:21:41.55 Sampled 1325.0ml @17:21:41.58 Drying puck before fixing for 4:00 @17:21:41.60 CBV.dial :waste2b @17:21:42.46 delay 1:00 @17:22:42.49 CSV.dial :air @17:22:42.96 CS.reconfigure CSfast @17:22:43.02 CS.seek 10ml @17:23:07.59 Pumping wcr puck from tube 3 up to 55psi... @17:23:07.61 CBV.dial SSV @17:23:08.99 CBV.dial between CSV and waste2b @17:23:10.03 CSV.dial between air and CTV @17:23:10.39 CS.seek 6.905ml @17:23:18.65 CTV.dial CSV,avoiding: :air @17:23:18.68 CSV.dial CTV @17:23:19.53 CS.reconfigure CSpressurize @17:23:19.59 CS.seek 0ml @17:23:37.89 CSV.dial :air @17:23:38.51 CS.reconfigure CSfast @17:23:38.57 CS.seek 10ml @17:24:03.06 CSV.dial between air and CTV @17:24:03.39 CS.seek 2.461ml @17:24:22.49 CTV.dial CSV,avoiding: :air @17:24:22.52 CSV.dial CTV @17:24:22.85 CS.reconfigure CSpressurize @17:24:22.91 CS.seek 0ml @17:24:27.14 CBV.dial :waste2b @17:24:27.54 CS.reconfigure CSconfig @17:24:27.59 CS.seek :empty @17:24:31.09 delay 1:00 @17:25:31.12 CTV.dial :air,avoiding: :intake @17:25:32.51 Fix #1 of 2 (with rnal) for 2:00 @17:25:32.53 CSV.dial CSR @17:25:33.69 Pulling 2ml of CSR.rnal @17:25:33.70 CSR.select :rnal @17:25:33.83 CS.seek 2ml @17:25:40.06 delay 5 seconds @17:25:45.08 CSR.select :air @17:25:45.21 CS.reconfigure CSfast @17:25:45.27 CS.seek 10ml @17:26:04.90 Pumping wcr puck from tube 3 up to 55psi... @17:26:04.91 CBV.dial SSV @17:26:06.28 CBV.dial between CSV and waste2b @17:26:07.33 CSV.dial between air and CTV @17:26:07.99 CS.seek 6.845ml @17:26:16.43 CTV.dial CSV,avoiding: :air @17:26:16.46 CSV.dial CTV @17:26:17.92 CS.reconfigure CSpressurize @17:26:17.98 CS.seek 0ml @17:26:36.12 CSV.dial :air @17:26:36.61 CS.reconfigure CSfast @17:26:36.67 CS.seek 10ml @17:27:00.97 CSV.dial between air and CTV @17:27:01.32 CS.seek 2.023ml @17:27:21.25 CTV.dial CSV,avoiding: :air @17:27:21.28 CSV.dial CTV @17:27:21.62 CS.reconfigure CSpressurize @17:27:21.68 CS.seek 0ml @17:27:23.02 delay 2:00 @17:29:23.04 CBV.dial :waste2b @17:29:23.41 CS.reconfigure CSconfig @17:29:23.46 CS.seek :empty @17:29:28.79 delay 1:00 @17:30:28.82 CTV.dial :air,avoiding: :intake @17:30:30.20 Fix #2 of 2 (with rnal) for 2:00 @17:30:30.22 CSV.dial CSR @17:30:30.75 Pulling 2ml of CSR.rnal @17:30:30.77 CSR.select :rnal @17:30:30.89 CS.seek 2ml @17:30:37.09 delay 5 seconds @17:30:42.11 CSR.select :air @17:30:42.24 CS.reconfigure CSfast @17:30:42.29 CS.seek 10ml @17:31:01.94 Pumping wcr puck from tube 3 up to 55psi... @17:31:01.96 CBV.dial SSV @17:31:03.94 CBV.dial between CSV and waste2b @17:31:04.99 CSV.dial between air and CTV @17:31:05.65 CS.seek 6.822ml @17:31:14.14 CTV.dial CSV,avoiding: :air @17:31:14.16 CSV.dial CTV @17:31:15.64 CS.reconfigure CSpressurize @17:31:15.70 CS.seek 0ml @17:31:33.68 delay 2:00 @17:33:33.71 CBV.dial :waste2b @17:33:34.07 CS.reconfigure CSconfig @17:33:34.13 CS.seek :empty @17:33:34.57 delay 1:00 @17:34:34.60 CTV.dial :air,avoiding: :intake @17:34:36.06 Storing wcr puck in Collection Clamp to tube 2 @17:34:36.26 SC.reconfigure SCtight @17:34:36.31 SC.seek 2 @17:34:38.48 SC.reconfigure SCloose @17:34:38.73 Elbow.reconfigure TightElbow @17:34:38.78 Elbow.seek 2 @17:34:40.68 Forearm.reconfigure TightForearm @17:34:40.74 Forearm.seek 2 + 700 counts @17:34:41.80 SE.reconfigure SEpick @17:34:41.88 SE.seek beamBreak @17:34:44.43 SE.configure SEconfig @17:34:44.60 Elbow.reconfigure LimpElbow @17:34:44.66 Forearm.reconfigure LooseForearm @17:34:44.71 Forearm.seek 2 @17:34:45.02 Elbow.hold @17:34:45.10 Elbow.reconfigure TightElbow @17:34:45.21 SE.seek 6.07 Pucks below Top Plate @17:34:45.77 Forearm.force -20 @17:34:45.81 Forearm.seek 2 + 341 counts @17:34:46.15 Forearm.reconfigure TightForearm @17:34:46.20 Forearm.seek 2 + 700 counts @17:34:46.44 SE.seek 7.07 Pucks below Top Plate @17:34:47.78 Forearm.reconfigure LooseForearm @17:34:47.84 Forearm.seek :retract @17:34:48.78 Elbow.seek CC @17:34:50.55 Elbow.reconfigure LooseElbow @17:34:50.67 Forearm.seek CC @17:34:51.64 Hand.close @17:34:52.26 CC.seek :open @17:34:56.81 Forearm.seek :retract @17:34:57.81 Elbow.reconfigure TightElbow @17:34:57.86 Elbow.seek 2 @17:34:59.76 Elbow.reconfigure LimpElbow @17:34:59.82 Forearm.seek 2 @17:35:00.84 Elbow.hold @17:35:00.92 Elbow.reconfigure TightElbow @17:35:00.98 Hand.open @17:35:01.79 Forearm.force -20 @17:35:01.83 Forearm.seek 2 + 350 counts @17:35:02.17 Forearm.reconfigure TightForearm @17:35:02.23 Forearm.seek 2 + 700 counts @17:35:02.53 SE.seek :down @17:35:03.68 Forearm.reconfigure LooseForearm @17:35:03.74 Forearm.seek :retract @17:35:04.87 SE.coast @17:35:04.91 SC.coast @17:35:04.96 Starting finalPurge @17:35:04.97 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:35:05.06 Hand.open @17:35:05.40 Elbow.seek FlushPuck::Garage @17:35:07.47 Elbow.reconfigure LooseElbow @17:35:07.58 Forearm.seek :garage @17:35:08.56 Hand.close @17:35:09.30 Forearm.seek :retract @17:35:10.31 Elbow.reconfigure TightElbow @17:35:10.36 Elbow.seek CC @17:35:13.27 Elbow.reconfigure LooseElbow @17:35:13.37 Forearm.reconfigure TightForearm @17:35:13.42 Forearm.seek CC @17:35:14.68 CC.seek :closed @17:35:18.93 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.05 @17:35:19.02 Hand.open @17:35:19.71 Forearm.reconfigure LooseForearm @17:35:19.76 Forearm.seek :retract @17:35:20.78 Flushing the Collection Valve::Manifold @17:35:20.93 CSV.dial CSR @17:35:21.46 Pulling 5ml of CSR.flush @17:35:21.47 CSR.select :flush @17:35:21.59 CS.seek 5ml @17:35:35.38 delay 5 seconds @17:35:40.40 CSR.select :air @17:35:40.59 CS.reconfigure CSfast @17:35:40.64 CS.seek 7.5ml @17:35:47.43 CSV.dial :waste @17:35:48.80 CS.reconfigure CSconfig @17:35:48.85 CS.seek 0ml @17:36:09.04 Flushing lower manifold's waste1 port @17:36:09.18 CSV.dial :flush @17:36:09.65 CS.reconfigure CSfast @17:36:09.70 CS.seek 5ml @17:36:22.35 delay 5 seconds @17:36:27.36 CSV.dial :air @17:36:27.94 CS.seek 7ml @17:36:33.60 CSV.dial CTV @17:36:33.63
CTV.dial CSV @17:36:35.10 CBV.dial :waste1 @17:36:35.94 CS.seek 0ml @17:36:53.16 Flushing lower manifold's waste2b port @17:36:53.30 CSV.dial :flush @17:36:54.09 CS.seek 5ml @17:37:06.72 delay 5 seconds @17:37:11.73 CSV.dial :air @17:37:12.29 CS.seek 7ml @17:37:18.07 CSV.dial CTV @17:37:18.10
CTV.dial CSV @17:37:18.58 CBV.dial :waste2b @17:37:19.41 CS.seek 0ml @17:37:36.63 Flushing upper manifold @17:37:36.77 CSV.dial :flush @17:37:37.56 CS.seek 5ml @17:37:50.10 delay 5 seconds @17:37:55.11 CSV.dial :air @17:37:55.67 CS.seek 7ml @17:38:01.41 CSV.dial CBV @17:38:01.44
CBV.dial CSV @17:38:02.58 CTV.dial :air @17:38:03.99 CS.seek 0ml @17:38:21.29 delay 7 seconds @17:38:28.32 CS.seek 3ml @17:38:36.37 CSV.dial :waste @17:38:37.52 CS.seek 0ml @17:38:45.64 CSV.dial :flush @17:38:46.11 CS.seek 3ml @17:38:54.23 delay 5 seconds @17:38:59.26 CSV.dial :air @17:38:59.82 CS.seek 5ml @17:39:05.48 CSV.dial :air @17:39:05.50 CS.seek 0ml @17:39:18.04 delay 7 seconds @17:39:25.07 CS.seek 3ml @17:39:33.04 CSV.dial :waste @17:39:33.85 CS.seek 0ml @17:39:41.79 Disinfecting external Intake with mfbkill @17:39:41.93 CSV.dial CSR @17:39:43.35 Pulling 8ml of CSR.mfbkill @17:39:43.36 CSR.select :mfbkill @17:39:43.49 CS.seek 8ml @17:40:03.07 delay 5 seconds @17:40:08.09 CSR.select :air @17:40:08.23 CS.seek 10ml @17:40:14.16 CSV.dial CBV @17:40:14.19
CBV.dial CSV @17:40:15.27 CTV.dial :intake @17:40:16.07 Intake.select :open @17:40:16.19 CS.seek 2ml @17:40:36.56 Intake.select :close @17:40:36.68 CTV.dial :air @17:40:37.49 CS.reconfigure CSconfig @17:40:37.54 CS.seek 0ml @17:40:43.73 Flushing lower manifold's waste1 port @17:40:43.74 CSV.dial CSR @17:40:44.88 Pulling 4ml of CSR.mfbkill @17:40:44.89 CSR.select :mfbkill @17:40:45.02 CS.seek 4ml @17:40:56.35 delay 5 seconds @17:41:01.37 CSR.select :air @17:41:01.50 CS.reconfigure CSfast @17:41:01.55 CS.seek 10ml @17:41:16.51 CSV.dial CTV @17:41:16.54
CTV.dial CSV @17:41:18.02 CBV.dial :waste1 @17:41:18.52 CS.seek 0ml @17:41:42.79 Flushing lower manifold's waste2b port @17:41:42.80 CSV.dial CSR @17:41:43.33 Pulling 3.999ml of CSR.mfbkill @17:41:43.34 CSR.select :mfbkill @17:41:43.47 CS.reconfigure CSconfig @17:41:43.51 CS.seek 4ml @17:41:54.76 delay 5 seconds @17:41:59.77 CSR.select :air @17:41:59.90 CS.reconfigure CSfast @17:41:59.95 CS.seek 10ml @17:42:14.92 CSV.dial CTV @17:42:14.94
CTV.dial CSV @17:42:15.43 CBV.dial :waste2b @17:42:16.25 CS.seek 0ml @17:42:40.57 Killing upper manifold @17:42:40.58 CSV.dial CSR @17:42:41.75 Pulling 4ml of CSR.mfbkill @17:42:41.76 CSR.select :mfbkill @17:42:41.89 CS.reconfigure CSconfig @17:42:41.94 CS.seek 4ml @17:42:53.14 delay 5 seconds @17:42:58.16 CSR.select :air @17:42:58.28 CS.reconfigure CSfast @17:42:58.33 CS.seek 10ml @17:43:13.26 CSV.dial CBV @17:43:13.29
CBV.dial CSV @17:43:14.43 CTV.dial :air @17:43:15.83 CS.seek 0ml @17:43:39.89 delay 7 seconds @17:43:46.92 CS.seek 3ml @17:43:54.95 CSV.dial :waste @17:43:55.55 CS.seek 0ml @17:44:03.54 Flushing upper manifold @17:44:03.68 CSV.dial :flush @17:44:04.19 CS.seek 5ml @17:44:16.95 delay 5 seconds @17:44:21.98 CSV.dial :air @17:44:22.55 CS.seek 7ml @17:44:28.20 CSV.dial CBV @17:44:28.23
CBV.dial CSV @17:44:29.31 CTV.dial :air @17:44:29.33 CS.seek 0ml @17:44:46.48 delay 7 seconds @17:44:53.51 CS.seek 3ml @17:45:01.44 CSV.dial :waste @17:45:01.94 CS.seek 0ml @17:45:10.02 CSV.dial :flush @17:45:10.49 CS.seek 3ml @17:45:18.42 delay 5 seconds @17:45:23.43 CSV.dial :air @17:45:24.12 CS.seek 5ml @17:45:29.73 CSV.dial :air @17:45:29.75 CS.seek 0ml @17:45:42.26 delay 7 seconds @17:45:49.28 CS.seek 3ml @17:45:57.32 CSV.dial :waste @17:45:58.10 CS.seek 0ml @17:46:06.11 Flushing lower manifold's waste1 port @17:46:06.25 CSV.dial :flush @17:46:06.74 CS.seek 5ml @17:46:19.36 delay 5 seconds @17:46:24.37 CSV.dial :air @17:46:24.93 CS.seek 7ml @17:46:30.51 CSV.dial CTV @17:46:30.54
CTV.dial CSV @17:46:32.00 CBV.dial :waste1 @17:46:32.51 CS.seek 0ml @17:46:49.70 Flushing lower manifold's waste2b port @17:46:49.84 CSV.dial :flush @17:46:50.65 CS.seek 5ml @17:47:03.14 delay 5 seconds @17:47:08.70 CSV.dial :air @17:47:09.30 CS.seek 7ml @17:47:15.01 CSV.dial CTV @17:47:15.04
CTV.dial CSV @17:47:15.51 CBV.dial :waste2b @17:47:16.34 CS.seek 0ml @17:47:33.48 Disinfecting external Intake with kill @17:47:33.62 CSV.dial CSR @17:47:34.29 Pulling 8ml of CSR.kill @17:47:34.30 CSR.select :kill @17:47:34.42 CS.seek 8ml @17:47:53.86 delay 5 seconds @17:47:58.88 CSR.select :air @17:47:59.02 CS.seek 10ml @17:48:04.70 CSV.dial CBV @17:48:04.73
CBV.dial CSV @17:48:05.87 CTV.dial :intake @17:48:06.66 Intake.select :open @17:48:06.78 CS.seek 2ml @17:48:27.38 Intake.select :close @17:48:27.50 CTV.dial :air @17:48:28.30 CS.reconfigure CSconfig @17:48:28.35 CS.seek 0ml @17:48:34.51 CSV.dial CSR @17:48:35.68 Pulling 5ml of CSR.flush CSR.select :flush @17:48:35.81 CS.seek 5ml @17:48:49.52 delay 5 seconds @17:48:54.54 CSR.select :air @17:48:54.68 CS.seek 10ml @17:49:08.44 CSV.dial CBV @17:49:09.52 CS.seek 0ml @17:49:36.05 Garaging FlushPuck in Collection Clamp @17:49:36.13 Elbow.reconfigure TightElbow @17:49:36.19 Elbow.seek CC @17:49:36.48 Elbow.reconfigure LooseElbow @17:49:36.58 Forearm.seek CC @17:49:37.59 Hand.close @17:49:38.16 CC.seek :open @17:49:42.56 Forearm.seek :retract @17:49:43.56 Elbow.reconfigure TightElbow @17:49:43.61 Elbow.seek :garage @17:49:46.68 Elbow.reconfigure LooseElbow @17:49:46.79 Forearm.reconfigure TightForearm @17:49:46.84 Forearm.seek :garage @17:49:47.98 Hand.open @17:49:48.73 Forearm.reconfigure LooseForearm @17:49:48.78 Forearm.seek :retract @17:49:49.81 Starting initialPurge @17:49:49.82 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:49:49.90 Hand.open @17:49:50.23 Elbow.reconfigure TightElbow @17:49:50.28 Elbow.seek FlushPuck::Garage @17:49:50.40 Elbow.reconfigure LooseElbow @17:49:50.51 Forearm.seek :garage @17:49:51.53 Hand.close @17:49:52.92 Forearm.seek :retract @17:49:53.92 Elbow.reconfigure TightElbow @17:49:53.97 Elbow.seek CC @17:49:56.81 Elbow.reconfigure LooseElbow @17:49:56.92 Forearm.reconfigure TightForearm @17:49:56.97 Forearm.seek CC @17:49:58.19 CC.seek :closed @17:50:02.44 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.07 @17:50:02.53 Hand.open @17:50:03.23 Forearm.reconfigure LooseForearm @17:50:03.28 Forearm.seek :retract @17:50:04.28 Flushing the Collection Valve::Manifold @17:50:04.42 CSV.dial CSR @17:50:05.69 Pulling 5ml of CSR.flush @17:50:05.70 CSR.select :flush @17:50:05.83 CS.seek 5ml @17:50:19.58 delay 5 seconds @17:50:24.60 CSR.select :air @17:50:24.78 CS.reconfigure CSfast @17:50:24.84 CS.seek 7.5ml @17:50:31.60 CSV.dial :waste @17:50:33.08 CS.reconfigure CSconfig @17:50:33.13 CS.seek 0ml @17:50:53.24 Purging external Intake @17:50:53.38 CSV.dial :flush @17:50:53.88 CS.reconfigure CSfast @17:50:53.93 CS.seek 10ml @17:51:17.86 delay 5 seconds @17:51:22.88 CSV.dial CBV @17:51:22.92
CBV.dial CSV @17:51:23.72 CTV.dial :intake @17:51:24.53 Intake.select :open @17:51:24.65 CS.seek 0ml @17:51:49.73 Intake.select :close @17:51:49.85 CTV.dial :air @17:51:50.64 Flushing upper manifold @17:51:50.78 CSV.dial :flush @17:51:51.56 CS.seek 5ml @17:52:04.11 delay 5 seconds @17:52:09.12 CSV.dial :air @17:52:09.67 CS.seek 7ml @17:52:15.36 CSV.dial CBV @17:52:15.39
CBV.dial CSV @17:52:16.47 CTV.dial :air @17:52:16.49 CS.seek 0ml @17:52:33.49 delay 7 seconds @17:52:40.51 CS.seek 3ml @17:52:48.42 CSV.dial :waste @17:52:49.04 CS.seek 0ml @17:52:57.05 CSV.dial :flush @17:52:57.54 CS.seek 3ml @17:53:06.17 delay 5 seconds @17:53:11.20 CSV.dial :air @17:53:11.76 CS.seek 5ml @17:53:17.63 CSV.dial :air @17:53:17.66 CS.seek 0ml @17:53:30.39 delay 7 seconds @17:53:37.42 CS.seek 3ml @17:53:45.33 CSV.dial :waste @17:53:46.12 CS.seek 0ml @17:53:54.05 Garaging FlushPuck in Collection Clamp @17:53:54.13 Elbow.reconfigure TightElbow @17:53:54.18 Elbow.seek CC @17:53:54.42 Elbow.reconfigure LooseElbow @17:53:54.53 Forearm.seek CC @17:53:55.52 Hand.close @17:53:56.16 CC.seek :open @17:54:00.54 Forearm.seek :retract @17:54:01.55 Elbow.reconfigure TightElbow @17:54:01.60 Elbow.seek :garage @17:54:04.69 Elbow.reconfigure LooseElbow @17:54:04.80 Forearm.reconfigure TightForearm @17:54:04.85 Forearm.seek :garage @17:54:06.00 Hand.open @17:54:06.73 Forearm.reconfigure LooseForearm @17:54:06.78 Forearm.seek :retract @17:54:07.81 Completed [phasewithdil] begun 14:00:27 @17:54:07.83 Preparing to sleep until 14PM @17:54:07.88 deferred until MFBPCR finish @17:54:07.90
Waiting for MFBPCR til 14PM @17:54:07.93 Continuing at 14:00CEST10-Oct-18 @17:54:07.99 GoodNews.email "Then, in tube 3: coreNEG\n/home/esp/esp2/mission/18tundeployv2dil.rb:123\n{1 => 22, 2 => 8, 3 => 14}\nCan@17:54:07, 14.7C, 69% humidity, 14.3psia, 13.230V, 0.569A, 0.569A avg, 7.53W",Subject: "Waiting until 14:00CEST10-Oct-18" @18:29:34.83 PCR.off @18:29:36.40 PCR for Tt1 completed -- closed /var/log/esp/Tt118oct0916h1500ml.pcr @18:29:36.48 Cleaning PCR @18:29:36.49 AFV.select :carrierSolution @18:29:36.60 delay 2 seconds @18:29:38.63 AS.reconfigure ASconfig @18:29:38.68 AS.seek 0.5ml @18:29:48.97 delay 1 second @18:29:49.98 AFV.select :holdingCoil @18:29:50.09 ASV.dial :waste1 @18:29:50.47 ATV.dial :air3 @18:29:51.65 AS.reconfigure ASslow1 @18:29:51.70 AS.seek 0.51ml @18:29:53.99 delay 2 seconds @18:29:56.01 ATV.dial :bleach @18:29:56.61 AS.seek 0.81ml @18:30:57.26 delay 3 seconds @18:31:00.28 ATV.dial :air3 @18:31:00.82 delay 3 seconds @18:31:03.91 AS.seek 0.835ml @18:31:09.24 delay 3 seconds @18:31:12.26 ATV.dial :bleach @18:31:12.86 AS.seek 0.82ml @18:31:16.12 delay 3 seconds @18:31:19.14 ARV.dial :waste @18:31:19.73 delay 3 seconds @18:31:22.75 ATV.dial :ARV @18:31:23.61 AS.reconfigure ASconfig @18:31:23.66 AS.seek 0.47ml @18:31:44.06 AFV.select :carrierSolution @18:31:44.18 delay 2 seconds @18:31:46.21 AS.seek 1ml @18:32:16.98 delay 1 second @18:32:17.99 AFV.select :holdingCoil @18:32:18.10 delay 1 second @18:32:19.13 AS.reconfigure ASfast @18:32:19.18 AS.seek 0ml @18:32:46.53 AFV.select :carrierSolution @18:32:46.65 delay 2 seconds @18:32:48.68 AS.reconfigure ASconfig @18:32:48.73 AS.seek 1ml @18:33:46.46 delay 1 second @18:33:47.47 AFV.select :holdingCoil @18:33:47.58 delay 1 second @18:33:48.61 AS.reconfigure ASfast @18:33:48.66 AS.seek 0ml @18:34:15.98 AFV.select :carrierSolution @18:34:16.72 delay 2 seconds @18:34:18.75 AS.reconfigure ASconfig @18:34:18.80 AS.seek 1ml @18:35:16.60 delay 1 second @18:35:17.62 AFV.select :holdingCoil @18:35:17.73 delay 1 second @18:35:18.76 AS.reconfigure ASfast @18:35:18.81 AS.seek 0ml @18:35:46.15 ARV.dial between carrier and air1 @18:35:46.63 ATV.dial :air3 @18:35:47.84 AS.reconfigure ASslow1 @18:35:47.89 AS.seek 0.01ml @18:35:50.27 delay 2 seconds @18:35:52.29 ATV.dial :bleach @18:35:52.89 AS.seek 0.81ml @18:38:34.07 delay 3 seconds @18:38:37.09 ATV.dial :air3 @18:38:37.62 delay 3 seconds @18:38:40.71 AS.seek 0.835ml @18:38:46.01 delay 3 seconds @18:38:49.03 ATV.dial :bleach @18:38:49.63 AS.seek 0.82ml @18:38:52.95 delay 3 seconds @18:38:55.97 ATV.dial PCR @18:38:56.37 AS.reconfigure ASconfig @18:38:56.42 AS.seek :empty @18:39:43.85 PCR.program :bleachProfile @18:39:43.91 PCR program should complete in < 2:45 @18:39:43.92 PCR.run 10:00 @18:42:54.83 PCR.off @18:42:54.85 AFV.select :carrierSolution @18:42:54.97 delay 2 seconds @18:42:57.00 AS.seek 1ml @18:43:54.84 delay 1 second @18:43:55.85 AFV.select :holdingCoil @18:43:55.96 delay 1 second @18:43:56.99 AS.reconfigure ASfast @18:43:57.04 AS.seek 0ml @18:44:24.39 delay 3 seconds @18:44:27.41 AFV.select :carrierSolution @18:44:27.52 delay 2 seconds @18:44:29.55 AS.reconfigure ASconfig @18:44:29.61 AS.seek 1ml @18:45:27.35 delay 1 second @18:45:28.36 AFV.select :holdingCoil @18:45:28.47 delay 1 second @18:45:29.50 AS.reconfigure ASfast @18:45:29.55 AS.seek 0ml @18:45:56.90 delay 3 seconds @18:45:59.92 AFV.select :carrierSolution @18:46:00.03 delay 2 seconds @18:46:02.06 AS.reconfigure ASconfig @18:46:02.11 AS.seek 1ml @18:46:59.87 delay 1 second @18:47:00.88 AFV.select :holdingCoil @18:47:00.99 delay 1 second @18:47:02.02 AS.reconfigure ASfast @18:47:02.07 AS.seek 0ml @18:47:29.42 delay 3 seconds @18:47:32.44 AFV.select :carrierSolution @18:47:32.55 delay 2 seconds @18:47:34.58 AS.reconfigure ASconfig @18:47:34.64 AS.seek 1ml @18:48:32.42 delay 1 second @18:48:33.43 AFV.select :holdingCoil @18:48:33.54 delay 1 second @18:48:34.57 AS.reconfigure ASfast @18:48:34.62 AS.seek 0ml @18:49:01.96 delay 3 seconds @18:49:04.98 AFV.select :carrierSolution @18:49:05.10 delay 2 seconds @18:49:07.14 AS.reconfigure ASconfig @18:49:07.19 AS.seek 1ml @18:50:04.97 delay 1 second @18:50:05.98 AFV.select :holdingCoil @18:50:06.09 delay 1 second @18:50:07.12 AS.reconfigure ASnorm @18:50:07.17 AS.seek 0ml @18:50:57.91 Setting up PCR for Rp -- writing /var/log/esp/Rp18oct0918h1500ml.pcr @18:50:57.93 Building PCR Slug from ASV with MM2 mix, PP3 primer @18:50:57.94 Priming with carrier @18:50:57.95 ATV.dial ARV @18:50:58.53 ARV.dial :carrier @18:50:58.90 AS.seek 0.5ml @18:51:24.46 AFV.select :carrierSolution @18:51:24.58 delay 2 seconds @18:51:26.61 AS.reconfigure ASconfig @18:51:26.66 AS.seek 1ml @18:51:55.68 delay 1 second @18:51:56.70 AFV.select :holdingCoil @18:51:56.81 delay 1 second @18:51:57.83 ATV.dial :waste @18:51:59.36 AS.reconfigure ASnorm @18:51:59.41 AS.seek :empty @18:52:50.13 AFV.select :carrierSolution @18:52:50.25 delay 2 seconds @18:52:52.89 AS.reconfigure ASconfig @18:52:52.96 AS.seek 0.5ml @18:53:22.04 delay 1 second @18:53:23.06 AFV.select :holdingCoil @18:53:23.17 Building Slug @18:53:23.18 ATV.dial :ARV @18:53:24.79 delay 2 seconds @18:53:26.81 ARV.dial :air1 @18:53:27.21 delay 2 seconds @18:53:29.30 AS.reconfigure ASslow2 @18:53:29.35 AS.seek 0.506ml @18:53:35.58 delay 2 seconds @18:53:37.60 ARV.dial :MM2 @18:53:38.98 delay 2 seconds @18:53:41.01 Adding MM2 @18:53:41.09 AS.seek 0.524ml @18:53:59.33 delay 25 seconds @18:54:24.35 ARV.dial :PP3 @18:54:24.75 Adding PP3 @18:54:24.77 delay 2 seconds @18:54:26.85 AS.seek 0.53ml @18:54:33.14 delay 10 seconds @18:54:43.16 ARV.dial :air2 @18:54:43.53 adding ARV air @18:54:43.55 delay 2 seconds @18:54:45.63 AS.seek 0.536ml @18:54:52.06 delay 5 seconds @18:54:57.08 ARV.dial :carrier @18:54:58.28 delay 7 seconds @18:55:05.37 AS.seek 0.594ml @18:56:04.12 delay 5 seconds @18:56:09.14 ATV.dial :ASV @18:56:09.55 delay 2 seconds @18:56:11.64 AS.seek 0.6ml @18:56:17.89 delay 4 seconds @18:56:21.91 ATV.dial ARV @18:56:22.44 delay 2 seconds @18:56:24.46 ARV.dial :carrier @18:56:24.48 delay 2 seconds @18:56:26.57 AS.seek 0.65ml @18:57:17.08 delay 4 seconds @18:57:21.10 ATV.dial PCR @18:57:21.69 ASV.dial :waste1 @18:57:21.70 ARV.dial :air1 @18:57:22.09 Positioning Slug by 0.324ml @18:57:22.18 AS.seek 0.326ml @19:02:48.25 delay 5 seconds @19:02:53.27 ATV.dial ARV @19:02:54.00 ASV.dial :PCRlock @19:02:54.39 delay 5 seconds @19:02:59.42 Running Rp Profile60C300 thermocycling @19:02:59.43 PCR.program Profile60C300 @19:02:59.57 Profile60C300 should complete in < 1:45:21 @19:02:59.58 PCR.run 2:16:41.25 @20:24:52.58 PCR.off @20:24:54.15 PCR for Rp completed -- closed /var/log/esp/Rp18oct0918h1500ml.pcr @20:24:54.23 Cleaning PCR @20:24:54.24 AFV.select :carrierSolution @20:24:54.35 delay 2 seconds @20:24:56.38 AS.reconfigure ASconfig @20:24:56.43 AS.seek 0.5ml @20:25:06.73 delay 1 second @20:25:07.74 AFV.select :holdingCoil @20:25:07.85 ASV.dial :waste1 @20:25:08.23 ATV.dial :air3 @20:25:09.44 AS.reconfigure ASslow1 @20:25:09.49 AS.seek 0.51ml @20:25:11.75 delay 2 seconds @20:25:13.77 ATV.dial :bleach @20:25:14.36 AS.seek 0.81ml @20:26:14.95 delay 3 seconds @20:26:17.97 ATV.dial :air3 @20:26:18.50 delay 3 seconds @20:26:21.59 AS.seek 0.835ml @20:26:26.88 delay 3 seconds @20:26:29.90 ATV.dial :bleach @20:26:30.51 AS.seek 0.82ml @20:26:33.81 delay 3 seconds @20:26:36.83 ARV.dial :waste @20:26:37.42 delay 3 seconds @20:26:40.44 ATV.dial :ARV @20:26:41.28 AS.reconfigure ASconfig @20:26:41.34 AS.seek 0.47ml @20:27:01.75 AFV.select :carrierSolution @20:27:01.87 delay 2 seconds @20:27:03.90 AS.seek 1ml @20:27:34.65 delay 1 second @20:27:35.67 AFV.select :holdingCoil @20:27:35.86 delay 1 second @20:27:38.67 AS.reconfigure ASfast @20:27:39.07 AS.seek 0ml @20:28:06.46 AFV.select :carrierSolution @20:28:06.58 delay 2 seconds @20:28:08.61 AS.reconfigure ASconfig @20:28:08.66 AS.seek 1ml @20:29:06.45 delay 1 second @20:29:07.46 AFV.select :holdingCoil @20:29:07.57 delay 1 second @20:29:08.60 AS.reconfigure ASfast @20:29:08.65 AS.seek 0ml @20:29:35.99 AFV.select :carrierSolution @20:29:36.11 delay 2 seconds @20:29:38.14 AS.reconfigure ASconfig @20:29:38.19 AS.seek 1ml @20:30:35.98 delay 1 second @20:30:36.99 AFV.select :holdingCoil @20:30:37.10 delay 1 second @20:30:38.13 AS.reconfigure ASfast @20:30:38.18 AS.seek 0ml @20:31:05.53 ARV.dial between carrier and air1 @20:31:06.01 ATV.dial :air3 @20:31:07.23 AS.reconfigure ASslow1 @20:31:07.28 AS.seek 0.01ml @20:31:09.66 delay 2 seconds @20:31:11.67 ATV.dial :bleach @20:31:12.27 AS.seek 0.81ml @20:33:53.41 delay 3 seconds @20:33:56.43 ATV.dial :air3 @20:33:56.97 delay 3 seconds @20:34:00.06 AS.seek 0.835ml @20:34:05.37 delay 3 seconds @20:34:08.39 ATV.dial :bleach @20:34:08.99 AS.seek 0.82ml @20:34:12.28 delay 3 seconds @20:34:15.30 ATV.dial PCR @20:34:15.69 AS.reconfigure ASconfig @20:34:15.74 AS.seek :empty @20:35:03.21 PCR.program :bleachProfile @20:35:03.28 PCR program should complete in < 2:45 @20:35:03.29 PCR.run 10:00 @20:38:14.20 PCR.off @20:38:14.22 AFV.select :carrierSolution @20:38:14.33 delay 2 seconds @20:38:16.36 AS.seek 1ml @20:39:14.17 delay 1 second @20:39:15.18 AFV.select :holdingCoil @20:39:15.29 delay 1 second @20:39:16.32 AS.reconfigure ASfast @20:39:16.37 AS.seek 0ml @20:39:43.69 delay 3 seconds @20:39:46.71 AFV.select :carrierSolution @20:39:46.82 delay 2 seconds @20:39:48.86 AS.reconfigure ASconfig @20:39:48.92 AS.seek 1ml @20:40:46.69 delay 1 second @20:40:47.70 AFV.select :holdingCoil @20:40:47.81 delay 1 second @20:40:48.84 AS.reconfigure ASfast @20:40:48.89 AS.seek 0ml @20:41:16.24 delay 3 seconds @20:41:19.26 AFV.select :carrierSolution @20:41:19.37 delay 2 seconds @20:41:21.40 AS.reconfigure ASconfig @20:41:21.46 AS.seek 1ml @20:42:19.24 delay 1 second @20:42:20.25 AFV.select :holdingCoil @20:42:20.36 delay 1 second @20:42:21.39 AS.reconfigure ASfast @20:42:21.44 AS.seek 0ml @20:42:48.80 delay 3 seconds @20:42:51.82 AFV.select :carrierSolution @20:42:51.93 delay 2 seconds @20:42:53.96 AS.reconfigure ASconfig @20:42:54.01 AS.seek 1ml @20:43:51.78 delay 1 second @20:43:52.79 AFV.select :holdingCoil @20:43:52.90 delay 1 second @20:43:53.93 AS.reconfigure ASfast @20:43:53.98 AS.seek 0ml @20:44:21.32 delay 3 seconds @20:44:24.34 AFV.select :carrierSolution @20:44:24.45 delay 2 seconds @20:44:26.48 AS.reconfigure ASconfig @20:44:26.53 AS.seek 1ml @20:45:24.30 delay 1 second @20:45:25.31 AFV.select :holdingCoil @20:45:25.42 delay 1 second @20:45:26.45 AS.reconfigure ASnorm @20:45:26.50 AS.seek 0ml @20:46:17.24 Setting up PCR for Bb -- writing /var/log/esp/Bb18oct0920h1500ml.pcr @20:46:17.26 Building PCR Slug from ASV with MM3 mix, PP5 primer @20:46:17.27 Priming with carrier @20:46:17.28 ATV.dial ARV @20:46:17.85 ARV.dial :carrier @20:46:18.20 AS.seek 0.5ml @20:46:43.74 AFV.select :carrierSolution @20:46:43.86 delay 2 seconds @20:46:46.50 AS.reconfigure ASconfig @20:46:46.57 AS.seek 1ml @20:47:15.60 delay 1 second @20:47:16.61 AFV.select :holdingCoil @20:47:16.72 delay 1 second @20:47:17.74 ATV.dial :waste @20:47:19.39 AS.reconfigure ASnorm @20:47:19.44 AS.seek :empty @20:48:10.21 AFV.select :carrierSolution @20:48:10.33 delay 2 seconds @20:48:12.36 AS.reconfigure ASconfig @20:48:12.41 AS.seek 0.5ml @20:48:41.49 delay 1 second @20:48:42.50 AFV.select :holdingCoil @20:48:42.61 Building Slug @20:48:42.62 ATV.dial :ARV @20:48:44.11 delay 2 seconds @20:48:46.13 ARV.dial :air1 @20:48:46.52 delay 2 seconds @20:48:48.60 AS.reconfigure ASslow2 @20:48:48.66 AS.seek 0.506ml @20:48:54.97 delay 2 seconds @20:48:56.99 ARV.dial :MM3 @20:48:57.97 delay 2 seconds @20:49:00.00 Adding MM3 @20:49:00.08 AS.seek 0.524ml @20:49:18.44 delay 25 seconds @20:49:43.46 ARV.dial :PP5 @20:49:43.85 Adding PP5 @20:49:43.87 delay 2 seconds @20:49:45.95 AS.seek 0.53ml @20:49:52.16 delay 10 seconds @20:50:02.18 ARV.dial :air3 @20:50:02.55 adding ARV air @20:50:02.57 delay 2 seconds @20:50:04.65 AS.seek 0.536ml @20:50:11.04 delay 5 seconds @20:50:16.06 ARV.dial :carrier @20:50:17.26 delay 7 seconds @20:50:24.35 AS.seek 0.594ml @20:51:23.27 delay 5 seconds @20:51:28.29 ATV.dial :ASV @20:51:28.68 delay 2 seconds @20:51:30.77 AS.seek 0.6ml @20:51:37.05 delay 4 seconds @20:51:41.07 ATV.dial ARV @20:51:41.69 delay 2 seconds @20:51:43.71 ARV.dial :carrier @20:51:43.73 delay 2 seconds @20:51:45.82 AS.seek 0.65ml @20:52:36.37 delay 4 seconds @20:52:40.39 ATV.dial PCR @20:52:40.99 ASV.dial :waste1 @20:52:41.00 ARV.dial :air1 @20:52:41.38 Positioning Slug by 0.324ml @20:52:41.47 AS.seek 0.326ml @20:58:07.52 delay 5 seconds @20:58:12.54 ATV.dial ARV @20:58:13.27 ASV.dial :PCRlock @20:58:13.65 delay 5 seconds @20:58:18.68 Running Bb Profile60C300 thermocycling @20:58:18.69 PCR.program Profile60C300 @20:58:18.83 Profile60C300 should complete in < 1:45:21 @20:58:18.84 PCR.run 2:16:41.25 @22:20:16.58 PCR.off @22:20:18.14 PCR for Bb completed -- closed /var/log/esp/Bb18oct0920h1500ml.pcr @22:20:18.21 Cleaning PCR @22:20:18.22 AFV.select :carrierSolution @22:20:18.33 delay 2 seconds @22:20:20.36 AS.reconfigure ASconfig @22:20:20.41 AS.seek 0.5ml @22:20:30.72 delay 1 second @22:20:31.73 AFV.select :holdingCoil @22:20:31.84 ASV.dial :waste1 @22:20:32.22 ATV.dial :air3 @22:20:33.42 AS.reconfigure ASslow1 @22:20:33.47 AS.seek 0.51ml @22:20:35.82 delay 2 seconds @22:20:37.84 ATV.dial :bleach @22:20:38.43 AS.seek 0.81ml @22:21:39.07 delay 3 seconds @22:21:42.09 ATV.dial :air3 @22:21:42.61 delay 3 seconds @22:21:45.69 AS.seek 0.835ml @22:21:51.02 delay 3 seconds @22:21:54.03 ATV.dial :bleach @22:21:54.64 AS.seek 0.82ml @22:21:57.99 delay 3 seconds @22:22:01.01 ARV.dial :waste @22:22:02.19 delay 3 seconds @22:22:05.23 ATV.dial :ARV @22:22:06.08 AS.reconfigure ASconfig @22:22:06.13 AS.seek 0.47ml @22:22:26.56 AFV.select :carrierSolution @22:22:26.68 delay 2 seconds @22:22:28.71 AS.seek 1ml @22:22:59.51 delay 1 second @22:23:00.52 AFV.select :holdingCoil @22:23:00.63 delay 1 second @22:23:01.66 AS.reconfigure ASfast @22:23:01.71 AS.seek 0ml @22:23:29.06 AFV.select :carrierSolution @22:23:29.18 delay 2 seconds @22:23:31.21 AS.reconfigure ASconfig @22:23:31.26 AS.seek 1ml @22:24:29.02 delay 1 second @22:24:30.03 AFV.select :holdingCoil @22:24:30.14 delay 1 second @22:24:31.17 AS.reconfigure ASfast @22:24:31.22 AS.seek 0ml @22:24:58.56 AFV.select :carrierSolution @22:24:58.68 delay 2 seconds @22:25:00.71 AS.reconfigure ASconfig @22:25:00.76 AS.seek 1ml @22:25:58.51 delay 1 second @22:25:59.52 AFV.select :holdingCoil @22:25:59.63 delay 1 second @22:26:00.66 AS.reconfigure ASfast @22:26:00.71 AS.seek 0ml @22:26:28.04 ARV.dial between carrier and air1 @22:26:28.52 ATV.dial :air3 @22:26:29.73 AS.reconfigure ASslow1 @22:26:29.78 AS.seek 0.01ml @22:26:32.12 delay 2 seconds @22:26:34.13 ATV.dial :bleach @22:26:34.80 AS.seek 0.81ml @22:29:15.96 delay 3 seconds @22:29:18.98 ATV.dial :air3 @22:29:19.51 delay 3 seconds @22:29:22.60 AS.seek 0.835ml @22:29:27.94 delay 3 seconds @22:29:30.96 ATV.dial :bleach @22:29:31.56 AS.seek 0.82ml @22:29:34.87 delay 3 seconds @22:29:37.89 ATV.dial PCR @22:29:38.27 AS.reconfigure ASconfig @22:29:38.32 AS.seek :empty @22:30:25.78 PCR.program :bleachProfile @22:30:25.84 PCR program should complete in < 2:45 @22:30:25.85 PCR.run 10:00 @22:33:36.77 PCR.off @22:33:36.79 AFV.select :carrierSolution @22:33:36.90 delay 2 seconds @22:33:38.93 AS.seek 1ml @22:34:36.73 delay 1 second @22:34:37.74 AFV.select :holdingCoil @22:34:37.85 delay 1 second @22:34:38.88 AS.reconfigure ASfast @22:34:38.93 AS.seek 0ml @22:35:06.29 delay 3 seconds @22:35:09.31 AFV.select :carrierSolution @22:35:09.42 delay 2 seconds @22:35:11.45 AS.reconfigure ASconfig @22:35:11.51 AS.seek 1ml @22:36:09.31 delay 1 second @22:36:10.32 AFV.select :holdingCoil @22:36:10.43 delay 1 second @22:36:11.46 AS.reconfigure ASfast @22:36:11.51 AS.seek 0ml @22:36:38.84 delay 3 seconds @22:36:41.86 AFV.select :carrierSolution @22:36:41.97 delay 2 seconds @22:36:44.00 AS.reconfigure ASconfig @22:36:44.05 AS.seek 1ml @22:37:41.82 delay 1 second @22:37:42.83 AFV.select :holdingCoil @22:37:42.94 delay 1 second @22:37:43.97 AS.reconfigure ASfast @22:37:44.02 AS.seek 0ml @22:38:11.35 delay 3 seconds @22:38:14.37 AFV.select :carrierSolution @22:38:14.48 delay 2 seconds @22:38:16.51 AS.reconfigure ASconfig @22:38:16.60 AS.seek 1ml @22:39:14.39 delay 1 second @22:39:15.40 AFV.select :holdingCoil @22:39:15.51 delay 1 second @22:39:16.54 AS.reconfigure ASfast @22:39:16.59 AS.seek 0ml @22:39:43.93 delay 3 seconds @22:39:46.95 AFV.select :carrierSolution @22:39:47.06 delay 2 seconds @22:39:49.09 AS.reconfigure ASconfig @22:39:49.14 AS.seek 1ml @22:40:46.93 delay 1 second @22:40:47.94 AFV.select :holdingCoil @22:40:48.05 delay 1 second @22:40:49.08 AS.reconfigure ASnorm @22:40:49.13 AS.seek 0ml @22:41:39.84 Setting up PCR for Ss -- writing /var/log/esp/Ss18oct0922h1500ml.pcr @22:41:40.49 Building PCR Slug from ASV with MM1 mix, PP2 primer @22:41:40.50 Priming with carrier @22:41:40.51 ATV.dial ARV @22:41:41.09 ARV.dial :carrier @22:41:41.46 AS.seek 0.5ml @22:42:07.02 AFV.select :carrierSolution @22:42:07.14 delay 2 seconds @22:42:09.17 AS.reconfigure ASconfig @22:42:09.22 AS.seek 1ml @22:42:38.23 delay 1 second @22:42:39.24 AFV.select :holdingCoil @22:42:39.35 delay 1 second @22:42:40.37 ATV.dial :waste @22:42:41.90 AS.reconfigure ASnorm @22:42:41.95 AS.seek :empty @22:43:32.69 AFV.select :carrierSolution @22:43:32.81 delay 2 seconds @22:43:34.84 AS.reconfigure ASconfig @22:43:34.89 AS.seek 0.5ml @22:44:03.98 delay 1 second @22:44:04.99 AFV.select :holdingCoil @22:44:05.10 Building Slug @22:44:05.11 ATV.dial :ARV @22:44:06.72 delay 2 seconds @22:44:08.74 ARV.dial :air1 @22:44:09.15 delay 2 seconds @22:44:11.23 AS.reconfigure ASslow2 @22:44:11.29 AS.seek 0.506ml @22:44:17.54 delay 2 seconds @22:44:19.55 ARV.dial :MM1 @22:44:20.20 delay 2 seconds @22:44:22.23 Adding MM1 @22:44:22.30 AS.seek 0.524ml @22:44:40.75 delay 25 seconds @22:45:05.77 ARV.dial :PP2 @22:45:06.15 Adding PP2 @22:45:06.16 delay 2 seconds @22:45:08.25 AS.seek 0.53ml @22:45:14.78 delay 10 seconds @22:45:24.80 ARV.dial :air2 @22:45:25.26 adding ARV air @22:45:25.27 delay 2 seconds @22:45:27.36 AS.seek 0.536ml @22:45:33.56 delay 5 seconds @22:45:38.58 ARV.dial :carrier @22:45:39.78 delay 7 seconds @22:45:46.87 AS.seek 0.594ml @22:46:45.47 delay 5 seconds @22:46:50.49 ATV.dial :ASV @22:46:50.89 delay 2 seconds @22:46:52.98 AS.seek 0.6ml @22:46:59.56 delay 4 seconds @22:47:03.58 ATV.dial ARV @22:47:04.11 delay 2 seconds @22:47:06.13 ARV.dial :carrier @22:47:06.15 delay 2 seconds @22:47:08.24 AS.seek 0.65ml @22:47:58.92 delay 4 seconds @22:48:02.94 ATV.dial PCR @22:48:03.51 ASV.dial :waste1 @22:48:03.52 ARV.dial :air1 @22:48:03.90 Positioning Slug by 0.324ml @22:48:04.00 AS.seek 0.326ml @22:53:30.20 delay 5 seconds @22:53:35.22 ATV.dial ARV @22:53:35.95 ASV.dial :PCRlock @22:53:36.34 delay 5 seconds @22:53:41.37 Running Ss Profile60C350 thermocycling @22:53:41.38 PCR.program Profile60C350 @22:53:41.53 Profile60C350 should complete in < 1:48:05 @22:53:41.54 PCR.run 2:20:06.25 @00:16:01.23CEST10-Oct-18 PCR.off @00:16:03.27 PCR for Ss completed -- closed /var/log/esp/Ss18oct0922h1500ml.pcr @00:16:03.34 Cleaning PCR @00:16:03.35 AFV.select :carrierSolution @00:16:03.46 delay 2 seconds @00:16:05.49 AS.reconfigure ASconfig @00:16:05.54 AS.seek 0.5ml @00:16:15.84 delay 1 second @00:16:16.85 AFV.select :holdingCoil @00:16:16.96 ASV.dial :waste1 @00:16:18.04 ATV.dial :air3 @00:16:19.25 AS.reconfigure ASslow1 @00:16:19.30 AS.seek 0.51ml @00:16:21.59 delay 2 seconds @00:16:23.60 ATV.dial :bleach @00:16:24.21 AS.seek 0.81ml @00:17:24.81 delay 3 seconds @00:17:27.83 ATV.dial :air3 @00:17:28.36 delay 3 seconds @00:17:31.45 AS.seek 0.835ml @00:17:36.76 delay 3 seconds @00:17:39.78 ATV.dial :bleach @00:17:40.38 AS.seek 0.82ml @00:17:43.62 delay 3 seconds @00:17:46.64 ARV.dial :waste @00:17:47.23 delay 3 seconds @00:17:50.25 ATV.dial :ARV @00:17:51.09 AS.reconfigure ASconfig @00:17:51.15 AS.seek 0.47ml @00:18:11.55 AFV.select :carrierSolution @00:18:11.67 delay 2 seconds @00:18:13.71 AS.seek 1ml @00:18:44.48 delay 1 second @00:18:45.49 AFV.select :holdingCoil @00:18:45.60 delay 1 second @00:18:46.63 AS.reconfigure ASfast @00:18:46.68 AS.seek 0ml @00:19:14.00 AFV.select :carrierSolution @00:19:14.12 delay 2 seconds @00:19:16.15 AS.reconfigure ASconfig @00:19:16.20 AS.seek 1ml @00:20:14.00 delay 1 second @00:20:15.01 AFV.select :holdingCoil @00:20:15.12 delay 1 second @00:20:16.15 AS.reconfigure ASfast @00:20:16.20 AS.seek 0ml @00:20:43.54 AFV.select :carrierSolution @00:20:43.66 delay 2 seconds @00:20:45.69 AS.reconfigure ASconfig @00:20:45.74 AS.seek 1ml @00:21:43.53 delay 1 second @00:21:44.54 AFV.select :holdingCoil @00:21:44.65 delay 1 second @00:21:45.68 AS.reconfigure ASfast @00:21:45.73 AS.seek 0ml @00:22:13.07 ARV.dial between carrier and air1 @00:22:13.56 ATV.dial :air3 @00:22:14.77 AS.reconfigure ASslow1 @00:22:14.82 AS.seek 0.01ml @00:22:17.14 delay 2 seconds @00:22:19.15 ATV.dial :bleach @00:22:19.76 AS.seek 0.81ml @00:25:00.92 delay 3 seconds @00:25:03.94 ATV.dial :air3 @00:25:04.47 delay 3 seconds @00:25:07.56 AS.seek 0.835ml @00:25:12.85 delay 3 seconds @00:25:15.87 ATV.dial :bleach @00:25:16.48 AS.seek 0.82ml @00:25:19.78 delay 3 seconds @00:25:22.80 ATV.dial PCR @00:25:23.19 AS.reconfigure ASconfig @00:25:23.24 AS.seek :empty @00:26:10.69 PCR.program :bleachProfile @00:26:10.75 PCR program should complete in < 2:45 @00:26:10.76 PCR.run 10:00 @00:29:21.68 PCR.off @00:29:21.71 AFV.select :carrierSolution @00:29:21.82 delay 2 seconds @00:29:23.85 AS.seek 1ml @00:30:21.69 delay 1 second @00:30:22.70 AFV.select :holdingCoil @00:30:22.81 delay 1 second @00:30:23.84 AS.reconfigure ASfast @00:30:23.89 AS.seek 0ml @00:30:51.25 delay 3 seconds @00:30:54.27 AFV.select :carrierSolution @00:30:54.38 delay 2 seconds @00:30:56.41 AS.reconfigure ASconfig @00:30:56.47 AS.seek 1ml @00:31:54.27 delay 1 second @00:31:55.28 AFV.select :holdingCoil @00:31:55.39 delay 1 second @00:31:56.42 AS.reconfigure ASfast @00:31:56.47 AS.seek 0ml @00:32:23.81 delay 3 seconds @00:32:26.83 AFV.select :carrierSolution @00:32:27.58 delay 2 seconds @00:32:29.61 AS.reconfigure ASconfig @00:32:29.66 AS.seek 1ml @00:33:27.44 delay 1 second @00:33:28.45 AFV.select :holdingCoil @00:33:28.56 delay 1 second @00:33:29.59 AS.reconfigure ASfast @00:33:29.64 AS.seek 0ml @00:33:57.00 delay 3 seconds @00:34:00.02 AFV.select :carrierSolution @00:34:00.13 delay 2 seconds @00:34:02.16 AS.reconfigure ASconfig @00:34:02.21 AS.seek 1ml @00:35:00.00 delay 1 second @00:35:01.01 AFV.select :holdingCoil @00:35:01.12 delay 1 second @00:35:02.15 AS.reconfigure ASfast @00:35:02.20 AS.seek 0ml @00:35:29.53 delay 3 seconds @00:35:32.55 AFV.select :carrierSolution @00:35:32.66 delay 2 seconds @00:35:34.69 AS.reconfigure ASconfig @00:35:34.75 AS.seek 1ml @00:36:32.53 delay 1 second @00:36:33.54 AFV.select :holdingCoil @00:36:33.65 delay 1 second @00:36:34.68 AS.reconfigure ASnorm @00:36:34.73 AS.seek 0ml @00:37:25.43 Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1000h1500ml.pcr @00:37:25.45 Building PCR Slug from ASV with MM1 mix, PP1 primer @00:37:25.46 Priming with carrier @00:37:25.47 ATV.dial ARV @00:37:26.16 ARV.dial :carrier @00:37:26.51 AS.seek 0.5ml @00:37:52.09 AFV.select :carrierSolution @00:37:52.21 delay 2 seconds @00:37:54.24 AS.reconfigure ASconfig @00:37:54.29 AS.seek 1ml @00:38:23.33 delay 1 second @00:38:24.35 AFV.select :holdingCoil @00:38:24.46 delay 1 second @00:38:25.48 ATV.dial :waste @00:38:27.00 AS.reconfigure ASnorm @00:38:27.05 AS.seek :empty @00:39:17.79 AFV.select :carrierSolution @00:39:17.91 delay 2 seconds @00:39:19.94 AS.reconfigure ASconfig @00:39:20.00 AS.seek 0.5ml @00:39:49.11 delay 1 second @00:39:50.13 AFV.select :holdingCoil @00:39:50.24 Building Slug @00:39:50.25 ATV.dial :ARV @00:39:51.75 delay 2 seconds @00:39:53.77 ARV.dial :air1 @00:39:54.16 delay 2 seconds @00:39:56.24 AS.reconfigure ASslow2 @00:39:56.30 AS.seek 0.506ml @00:40:02.50 delay 2 seconds @00:40:04.52 ARV.dial :MM1 @00:40:05.10 delay 2 seconds @00:40:07.13 Adding MM1 @00:40:07.20 AS.seek 0.524ml @00:40:25.50 delay 25 seconds @00:40:50.52 ARV.dial :PP1 @00:40:50.91 Adding PP1 @00:40:50.92 delay 2 seconds @00:40:53.01 AS.seek 0.53ml @00:40:59.41 delay 10 seconds @00:41:09.43 ARV.dial :air1 @00:41:09.80 adding ARV air @00:41:09.81 delay 2 seconds @00:41:11.90 AS.seek 0.536ml @00:41:18.40 delay 5 seconds @00:41:23.42 ARV.dial :carrier @00:41:23.80 delay 7 seconds @00:41:30.89 AS.seek 0.594ml @00:42:29.44 delay 5 seconds @00:42:34.46 ATV.dial :ASV @00:42:34.85 delay 2 seconds @00:42:36.94 AS.seek 0.6ml @00:42:43.11 delay 4 seconds @00:42:47.13 ATV.dial ARV @00:42:47.76 delay 2 seconds @00:42:49.78 ARV.dial :carrier @00:42:49.80 delay 2 seconds @00:42:51.89 AS.seek 0.65ml @00:43:42.40 delay 4 seconds @00:43:46.43 ATV.dial PCR @00:43:47.12 ASV.dial :waste1 @00:43:47.13 ARV.dial :air1 @00:43:47.52 Positioning Slug by 0.324ml @00:43:47.61 AS.seek 0.326ml @00:49:13.70 delay 5 seconds @00:49:18.72 ATV.dial ARV @00:49:19.45 ASV.dial :PCRlock @00:49:19.82 delay 5 seconds @00:49:24.85 Running IPc ProfileIPc thermocycling @00:49:24.86 PCR.program ProfileIPc @00:49:25.01 ProfileIPc should complete in < 1:50:49 @00:49:25.02 PCR.run 2:23:31.25 @02:13:45.96 PCR.off @02:13:48.62 PCR for IPc completed -- closed /var/log/esp/IPc18oct1000h1500ml.pcr @02:13:48.64 Full MFB Decontamination @02:13:48.65 Decon extraction column and decon AIV @02:13:48.67 SPE.hold 2:46:40,55C @02:13:48.73 ASV.dial :SPEdelivery @02:13:49.33 delay 20 seconds @02:14:09.35 AFV.select :carrierSolution @02:14:09.46 delay 2 seconds @02:14:11.49 AS.reconfigure ASconfig @02:14:11.54 AS.seek 0.5ml @02:14:21.88 delay 1 second @02:14:22.89 AFV.select :holdingCoil @02:14:23.00 ATV.dial :air1 @02:14:24.75 AS.reconfigure ASslow1 @02:14:24.80 AS.seek 0.52ml @02:14:29.12 ATV.dial :ASV @02:14:30.77 ASV.dial :SPErecovery @02:14:31.42 AS.seek 0.62ml @02:14:51.78 ATV.dial :waste @02:14:53.47 AS.reconfigure ASnorm @02:14:53.52 AS.seek :empty @02:15:25.15 AFV.select :holdingCoil @02:15:25.17 ATV.dial :waste @02:15:25.19 AS.reconfigure ASconfig @02:15:25.24 AS.seek 0ml @02:15:25.56 AFV.select :carrierSolution @02:15:25.68 delay 2 seconds @02:15:27.71 AS.seek 0.5ml @02:15:56.71 delay 1 second @02:15:57.72 AFV.select :holdingCoil @02:15:57.83 ATV.dial :air3 @02:15:58.65 AS.reconfigure ASslow1 @02:15:58.70 AS.seek 0.52ml @02:16:02.98 ATV.dial :air3 @02:16:03.07 AS.seek 0.53ml @02:16:05.38 delay 2 seconds @02:16:07.39 ATV.dial :bleach @02:16:07.87 AS.seek 0.71ml @02:16:44.33 delay 3 seconds @02:16:47.35 ATV.dial :air3 @02:16:47.90 delay 3 seconds @02:16:50.99 AS.seek 0.735ml @02:16:56.27 delay 3 seconds @02:16:59.29 ATV.dial :bleach @02:16:59.90 AS.seek 0.72ml @02:17:03.22 delay 3 seconds @02:17:06.25 ATV.dial ASV @02:17:07.26 ASV.dial :SPErecovery @02:17:07.34 AS.seek 0.365ml @02:18:19.00 ATV.dial :waste @02:18:20.63 AS.reconfigure ASnorm @02:18:20.68 AS.seek :empty @02:18:39.45 ATV.dial :air3 @02:18:40.12 AS.reconfigure ASslow1 @02:18:40.17 AS.seek 0.009ml @02:18:42.51 delay 2 seconds @02:18:44.52 ATV.dial :bleach @02:18:45.00 AS.seek 0.579ml @02:20:39.91 delay 3 seconds @02:20:42.93 ATV.dial :air3 @02:20:43.46 delay 3 seconds @02:20:46.55 AS.seek 0.604ml @02:20:52.46 delay 3 seconds @02:20:55.48 ATV.dial :bleach @02:20:56.08 AS.seek 0.589ml @02:20:59.35 delay 3 seconds @02:21:02.37 ATV.dial :ASV @02:21:03.34 ASV.dial :wasteR @02:21:03.72 AS.seek 0ml @02:23:02.46 delay 1:00 @02:24:02.48 ATV.dial ASV @02:24:02.50 AFV.select :carrierSolution @02:24:02.60 delay 2 seconds @02:24:04.63 AS.reconfigure ASconfig @02:24:04.68 AS.seek 1ml @02:25:02.45 delay 1 second @02:25:03.46 AFV.select :holdingCoil @02:25:03.57 delay 1 second @02:25:04.59 ASV.dial :wasteR @02:25:04.61 AS.reconfigure ASslow1 @02:25:04.66 AS.seek 0ml @02:28:26.01 delay 10 seconds @02:28:36.03 ATV.dial :air3 @02:28:37.44 AS.seek 0.01ml @02:28:39.79 delay 2 seconds @02:28:41.80 ATV.dial :bleach @02:28:42.45 AS.seek 0.58ml @02:30:37.34 delay 3 seconds @02:30:40.36 ATV.dial :air3 @02:30:40.89 delay 3 seconds @02:30:43.98 AS.seek 0.605ml @02:30:49.26 delay 3 seconds @02:30:52.28 ATV.dial :bleach @02:30:52.88 AS.seek 0.59ml @02:30:56.15 delay 3 seconds @02:30:59.18 ATV.dial ASV @02:31:00.14 ASV.dial :SPEdelivery @02:31:00.50 AS.seek 0ml @02:32:59.39 delay 1:00 @02:33:59.41 AFV.select :carrierSolution @02:33:59.52 delay 2 seconds @02:34:01.55 AS.reconfigure ASconfig @02:34:01.60 AS.seek 1ml @02:34:59.42 delay 1 second @02:35:00.43 AFV.select :holdingCoil @02:35:00.54 delay 1 second @02:35:01.56 ATV.dial ASV @02:35:01.58 ASV.dial :SPEdelivery @02:35:01.60 AS.reconfigure ASslow1 @02:35:01.64 AS.seek 0ml @02:38:23.04 delay 10 seconds @02:38:33.06 ATV.dial :air2 @02:38:33.84 AS.reconfigure ASslow0 @02:38:33.89 AS.seek 0.8ml @02:39:54.68 delay 3 seconds @02:39:57.70 ATV.dial :ASV @02:39:58.62 ASV.dial :SPEdelivery @02:39:58.64 AS.reconfigure ASslow1 @02:39:58.69 AS.seek 0.03ml @02:42:33.81 delay 20 seconds @02:42:53.84 ATV.dial :waste,via: :air3 @02:42:55.42 AS.reconfigure ASconfig @02:42:55.47 AS.seek :empty @02:42:57.54 SPE.coast @02:42:57.63 Removing Bleach from Mixing Coil @02:42:57.64 AFV.select :carrierSolution @02:42:57.75 delay 2 seconds @02:42:59.78 AS.seek 0.5ml @02:43:28.90 delay 1 second @02:43:29.91 AFV.select :holdingCoil @02:43:30.02 ATV.dial :ASV @02:43:31.58 ASV.dial :SPErecovery @02:43:32.21 AS.reconfigure ASslow1 @02:43:32.26 AS.seek 0.73ml @02:44:18.75 ASV.dial :wasteD @02:44:19.30 AS.reconfigure ASnorm @02:44:19.35 AS.seek 0ml @02:44:56.47 AFV.select :carrierSolution @02:44:56.59 delay 2 seconds @02:44:58.62 AS.reconfigure ASconfig @02:44:58.67 AS.seek 0.5ml @02:45:27.73 delay 1 second @02:45:28.74 AFV.select :holdingCoil @02:45:28.85 ATV.dial ASV @02:45:28.86 ASV.dial :SPErecovery @02:45:29.49 AS.reconfigure ASslow0 @02:45:29.54 AS.seek 0.3ml @02:45:49.96 ATV.dial :air2 @02:45:50.96 AS.reconfigure ASslow1 @02:45:51.01 AS.seek 0.32ml @02:45:55.35 ATV.dial ASV @02:45:56.33 AS.reconfigure ASslow0 @02:45:56.38 AS.seek 0.57ml @02:46:21.85 ASV.dial :wasteD @02:46:23.04 AS.reconfigure ASnorm @02:46:23.11 AS.seek 0ml @02:46:52.19 AFV.select :carrierSolution @02:46:52.31 delay 2 seconds @02:46:54.34 AS.reconfigure ASconfig @02:46:54.39 AS.seek 0.5ml @02:47:23.41 delay 1 second @02:47:24.43 AFV.select :holdingCoil @02:47:24.54 ATV.dial ASV @02:47:24.55 ASV.dial :SPErecovery @02:47:25.18 AS.reconfigure ASslow0 @02:47:25.23 AS.seek 0.3ml @02:47:45.70 ATV.dial :air2 @02:47:46.68 AS.reconfigure ASslow1 @02:47:46.73 AS.seek 0.32ml @02:47:50.97 ATV.dial ASV @02:47:51.96 AS.reconfigure ASslow0 @02:47:52.01 AS.seek 0.57ml @02:48:17.46 ASV.dial :wasteD @02:48:18.03 AS.reconfigure ASnorm @02:48:18.08 AS.seek 0ml @02:48:47.15 ATV.dial :air2 @02:48:48.08 AS.reconfigure ASslow0 @02:48:48.13 AS.seek 0.3ml @02:49:18.63 ATV.dial :ASV @02:49:19.56 ASV.dial :wasteD @02:49:19.58 AS.reconfigure ASconfig @02:49:19.63 AS.seek 0.05ml @02:49:34.32 ATV.dial :waste @02:49:36.05 AS.seek 0ml @02:49:39.21 ASV.dial :SPEdelivery @02:49:40.16 deconLysateINT @02:49:40.17 ATV.dial :air3 @02:49:40.84 AS.reconfigure ASslow1 @02:49:40.89 AS.seek 0.01ml @02:49:43.23 delay 2 seconds @02:49:45.25 ATV.dial :bleach @02:49:45.73 AS.seek 0.96ml @02:52:57.09 delay 3 seconds @02:53:00.11 ATV.dial :air3 @02:53:00.64 delay 3 seconds @02:53:03.73 AS.seek 0.985ml @02:53:09.02 delay 3 seconds @02:53:12.04 ATV.dial :bleach @02:53:12.63 AS.seek 0.97ml @02:53:15.92 delay 3 seconds @02:53:18.95 ATV.dial AIV @02:53:20.11 AIV.dial :coilToMFB @02:53:20.12 AWV.select :waste @02:53:20.13 AFV.select :carrierSolution @02:53:20.25 delay 2 seconds @02:53:22.28 AS.reconfigure ASconfig @02:53:22.33 AS.seek 1ml @02:53:24.39 delay 1 second @02:53:25.40 AFV.select :holdingCoil @02:53:25.51 delay 1 second @02:53:26.54 AS.reconfigure ASslow0 @02:53:26.58 AS.seek :empty @02:55:07.57 AFV.select :carrierSolution @02:55:07.69 delay 2 seconds @02:55:09.72 AS.reconfigure ASconfig @02:55:09.77 AS.seek 1ml @02:56:07.58 delay 1 second @02:56:08.59 AFV.select :holdingCoil @02:56:08.71 delay 1 second @02:56:09.74 AS.reconfigure ASslow0 @02:56:09.78 AS.seek :empty @02:57:50.76 AFV.select :carrierSolution @02:57:50.88 delay 2 seconds @02:57:52.91 AS.reconfigure ASconfig @02:57:52.96 AS.seek 1ml @02:58:50.77 delay 1 second @02:58:51.78 AFV.select :holdingCoil @02:58:51.89 delay 1 second @02:58:52.92 AS.reconfigure ASslow0 @02:58:52.96 AS.seek :empty @03:00:33.93 ATV.dial :air2 @03:00:34.59 AS.reconfigure ASslow1 @03:00:34.64 AS.seek 0.009ml @03:00:36.92 AS.reconfigure ASslow0 @03:00:36.97 AS.seek 1ml @03:02:16.90 ATV.dial AIV @03:02:17.64 AS.seek 0.02ml @03:03:56.51 ATV.dial :waste @03:03:58.05 AS.seek 0ml @03:04:00.37 AWV.select :air @03:04:00.54 Cleaning PCR @03:04:00.55 AFV.select :carrierSolution @03:04:00.66 delay 2 seconds @03:04:02.69 AS.reconfigure ASconfig @03:04:02.74 AS.seek 0.5ml @03:04:31.82 delay 1 second @03:04:33.47 AFV.select :holdingCoil @03:04:33.58 ASV.dial :waste1 @03:04:33.96 ATV.dial :air3 @03:04:34.62 AS.reconfigure ASslow1 @03:04:34.67 AS.seek 0.51ml @03:04:36.95 delay 2 seconds @03:04:38.97 ATV.dial :bleach @03:04:39.53 AS.seek 0.81ml @03:05:40.11 delay 3 seconds @03:05:43.13 ATV.dial :air3 @03:05:43.66 delay 3 seconds @03:05:46.75 AS.seek 0.835ml @03:05:52.07 delay 3 seconds @03:05:55.09 ATV.dial :bleach @03:05:55.68 AS.seek 0.82ml @03:05:59.00 delay 3 seconds @03:06:02.02 ARV.dial :waste @03:06:02.61 delay 3 seconds @03:06:05.63 ATV.dial :ARV @03:06:06.57 AS.reconfigure ASconfig @03:06:06.62 AS.seek 0.47ml @03:06:27.01 AFV.select :carrierSolution @03:06:27.13 delay 2 seconds @03:06:29.16 AS.seek 1ml @03:06:59.94 delay 1 second @03:07:00.96 AFV.select :holdingCoil @03:07:01.07 delay 1 second @03:07:02.10 AS.reconfigure ASfast @03:07:02.15 AS.seek 0ml @03:07:29.48 AFV.select :carrierSolution @03:07:29.60 delay 2 seconds @03:07:31.63 AS.reconfigure ASconfig @03:07:31.68 AS.seek 1ml @03:08:29.47 delay 1 second @03:08:30.49 AFV.select :holdingCoil @03:08:30.60 delay 1 second @03:08:31.63 AS.reconfigure ASfast @03:08:31.68 AS.seek 0ml @03:08:59.01 AFV.select :carrierSolution @03:08:59.13 delay 2 seconds @03:09:01.16 AS.reconfigure ASconfig @03:09:01.21 AS.seek 1ml @03:09:59.02 delay 1 second @03:10:00.04 AFV.select :holdingCoil @03:10:00.15 delay 1 second @03:10:01.18 AS.reconfigure ASfast @03:10:01.23 AS.seek 0ml @03:10:28.57 ARV.dial between carrier and air1 @03:10:29.07 ATV.dial :air3 @03:10:30.26 AS.reconfigure ASslow1 @03:10:30.31 AS.seek 0.01ml @03:10:32.63 delay 2 seconds @03:10:34.64 ATV.dial :bleach @03:10:35.25 AS.seek 0.81ml @03:13:16.38 delay 3 seconds @03:13:19.40 ATV.dial :air3 @03:13:19.93 delay 3 seconds @03:13:23.02 AS.seek 0.835ml @03:13:28.31 delay 3 seconds @03:13:31.33 ATV.dial :bleach @03:13:31.94 AS.seek 0.82ml @03:13:35.23 delay 3 seconds @03:13:38.25 ATV.dial PCR @03:13:38.65 AS.reconfigure ASconfig @03:13:38.70 AS.seek :empty @03:14:26.18 PCR.program :bleachProfile @03:14:26.24 PCR program should complete in < 2:45 @03:14:26.25 PCR.run 10:00 @03:17:46.24 PCR.off @03:17:46.27 AFV.select :carrierSolution @03:17:46.38 delay 2 seconds @03:17:48.41 AS.seek 1ml @03:18:46.26 delay 1 second @03:18:47.27 AFV.select :holdingCoil @03:18:47.38 delay 1 second @03:18:48.41 AS.reconfigure ASfast @03:18:48.46 AS.seek 0ml @03:19:15.82 delay 3 seconds @03:19:18.84 AFV.select :carrierSolution @03:19:18.95 delay 2 seconds @03:19:20.98 AS.reconfigure ASconfig @03:19:21.03 AS.seek 1ml @03:20:18.87 delay 1 second @03:20:19.88 AFV.select :holdingCoil @03:20:19.99 delay 1 second @03:20:21.65 AS.reconfigure ASfast @03:20:21.70 AS.seek 0ml @03:20:49.06 delay 3 seconds @03:20:52.08 AFV.select :carrierSolution @03:20:52.19 delay 2 seconds @03:20:54.22 AS.reconfigure ASconfig @03:20:54.28 AS.seek 1ml @03:21:52.09 delay 1 second @03:21:53.11 AFV.select :holdingCoil @03:21:53.22 delay 1 second @03:21:54.25 AS.reconfigure ASfast @03:21:54.30 AS.seek 0ml @03:22:21.65 delay 3 seconds @03:22:24.67 AFV.select :carrierSolution @03:22:24.78 delay 2 seconds @03:22:26.81 AS.reconfigure ASconfig @03:22:26.86 AS.seek 1ml @03:23:24.65 delay 1 second @03:23:25.67 AFV.select :holdingCoil @03:23:25.78 delay 1 second @03:23:26.81 AS.reconfigure ASfast @03:23:26.86 AS.seek 0ml @03:23:54.21 delay 3 seconds @03:23:57.23 AFV.select :carrierSolution @03:23:57.34 delay 2 seconds @03:23:59.37 AS.reconfigure ASconfig @03:23:59.43 AS.seek 1ml @03:24:57.23 delay 1 second @03:24:58.25 AFV.select :holdingCoil @03:24:58.36 delay 1 second @03:24:59.39 AS.reconfigure ASnorm @03:24:59.44 AS.seek 0ml @03:25:50.15 AFV.select :carrierSolution @03:25:50.26 delay 2 seconds @03:25:52.29 AS.reconfigure ASconfig @03:25:52.34 AS.seek 1ml @03:26:50.10 delay 1 second @03:26:51.11 AFV.select :holdingCoil @03:26:51.22 delay 1 second @03:26:52.24 ATV.dial :waste @03:26:53.39 AS.reconfigure ASnorm @03:26:53.45 AS.seek 0.5ml @03:27:19.00 ATV.dial :waste2 @03:27:20.18 AS.seek 0ml @03:27:45.68 AS.coast @03:27:45.72 SS.coast @03:27:45.77 CC.coast @03:27:45.81 CS.coast @03:27:45.85 PC.coast @03:27:45.89 PS.coast @03:27:45.93 Forearm.coast @03:27:45.97 Elbow.coast @03:27:46.37 Gate.power :core,:OFF @03:27:46.38 Gate.power :raw,:OFF @03:27:46.39 Shutdown @03:27:46.41 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @09:39:08.93 Session Begun @09:41:09.86 -> abort! "Will implement tweaks. BKHA" @09:41:09.89 CmdServer::Client::Error in bkha -- Aborting Mission: Will implement tweaks. BKHA @09:41:09.97 BadNews.email "Will implement tweaks. BKHA",Subject: "18tundeployv2dil ABORTED!" @09:41:18.63 Session Ended @12:09:23.69CEST11-Oct-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @12:09:25.88 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @12:09:26.20 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @12:09:31.42 Polling Can every 10:00 @12:09:31.53 Cleanly exited at 09:41:18CEST10-Oct-18 @12:09:31.58 Loading /home/esp/esp2/mission/18tundeployv3.rb mission @12:09:31.65 GoodNews.email "Starting 18tundeployv3 w/pucks in tube 3\n{1 => 22, 2 => 8, 3 => 14}\nCan@12:09:31, 13.4C, 70% humidity, 14.3psia, 13.436V, 0.405A, 0.405A avg, 5.44W",Subject: "18tundeployv3 begun" @12:09:31.70
Preparing to sleep until 14:00 11-oct-18 @12:09:31.75 Shutdown @12:09:31.77
Asleep til 14:00 11-oct-18 @12:09:31.81 Continuing at 14:00 @12:09:54.04 Session Begun @12:09:54.78 GoodNews.email "Then, in tube 3: mbaPhase\n/home/esp/esp2/mission/18tundeployv3.rb:118\n{1 => 22, 2 => 8, 3 => 14}\nCan@12:09:31, 13.4C, 70% humidity, 14.3psia, 13.436V, 0.405A, 0.405A avg, 5.44W",Subject: "Asleep until 14:00" @12:10:15.86 -> exit @12:10:15.88 Session Ended @14:00:00.06
Powering core bus @14:00:05.14 Gate.power :raw,:ON @14:00:05.88 SPE.coast @14:00:05.94 SPE.configure SPEconfig @14:00:06.04 AS.configure ASconfig @14:00:06.14 SS.configure SSconfig @14:00:06.24 CC.configure CCconfig @14:00:06.34 CS.configure CSconfig @14:00:06.44 PC.configure PCconfig @14:00:06.54 PS.configure PSconfig @14:00:06.67 Forearm.configure LooseForearm @14:00:06.76 Elbow.configure LooseElbow @14:00:06.88 SC.configure SCloose @14:00:06.98 SE.configure SEconfig @14:00:07.03 CH.coast @14:00:07.09 CH.configure CHconfig @14:00:07.12 PH.coast @14:00:07.19 PH.configure PHconfig @14:00:07.23 PCR.off @14:00:07.26 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:07.29 PCR.caltemp= 2.7073,0.0373,0 @14:00:07.32 All dwarf controllers running firmware version 3.75 @14:00:07.44 Valve.reset @14:00:07.61 PCR.off @14:00:07.64 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:07.67 PCR.caltemp= 2.7073,0.0373,0 @14:00:07.91 PSV.dial :air @14:00:07.93 Intake.select :close @14:00:07.99 Exhaust.select :close @14:00:08.00 CTV.dial :air @14:00:08.09 CSV.dial :waste1 @14:00:08.21
Forearm.reconfigure LooseForearm @14:00:08.28 SSV.dial :spit,avoiding: :exhaust @14:00:08.30
Forearm.home.seek :retract @14:00:08.44 PS.reconfigure PSconfig @14:00:08.53 PS.home.seek 0ml @14:00:09.10 Hand.close @14:00:09.17
Elbow.jog 1500 @14:00:09.28 CS.reconfigure CSfast @14:00:09.31 SS.reconfigure SSconfig @14:00:09.42 CS.home.seek 0ml @14:00:09.46 SS.home.seek 0ml @14:00:09.81
Elbow.reconfigure LooseElbow @14:00:09.86 Elbow.home.seek :clear @14:00:13.23 SE.reconfigure SEconfig @14:00:13.30 SE.home.seek :down @14:00:14.03 SC.reconfigure SCsloppy @14:00:14.08 SC.home.seek :initial @14:00:17.55 PS.coast @14:00:18.08
Forearm.seek :retract @14:00:18.17 Elbow.seek :hammer @14:00:19.35 Forearm.reconfigure TightForearm @14:00:19.40 Forearm.seek 5 + 350 counts @14:00:20.51 Forearm.seek :retract @14:00:22.14 PC.reconfigure PCconfig @14:00:22.15
CC.reconfigure CCconfig @14:00:22.25 PC.home.seek :open @14:00:22.27
CC.home.seek :open @14:00:25.57 Phase [mbaPhase] @14:00:25.59 GoodNews.email "At 14:00:25 began in tube 3: mbaPhase\n/home/esp/esp2/mission/18tundeployv3.rb:118\n{1 => 22, 2 => 8, 3 => 14}\nCan@14:00:25, 13.4C, 70% humidity, 14.3psia, 13.294V, 0.979A, 0.979A avg, 13.01W",Subject: "Started 18tundeployv3 phase mbaPhase" @14:00:25.62CEST11-Oct-18
==================> STARTED Superphase (phase #1) <=== @14:00:25.65 Starting initialPurge @14:00:25.66 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:00:25.74 Hand.open @14:00:26.49 Elbow.reconfigure TightElbow @14:00:26.53 Elbow.seek FlushPuck::Garage @14:00:28.48 Elbow.reconfigure LooseElbow @14:00:28.61 Forearm.reconfigure LooseForearm @14:00:28.66 Forearm.seek :garage @14:00:29.64 Hand.close @14:00:30.46 Forearm.seek :retract @14:00:31.44 Elbow.reconfigure TightElbow @14:00:31.50 Elbow.seek CC @14:00:34.41 Elbow.reconfigure LooseElbow @14:00:34.56 Forearm.reconfigure TightForearm @14:00:34.65 Forearm.seek CC @14:00:35.91 CC.seek :closed @14:00:40.16 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.10 @14:00:40.23 Hand.open @14:00:40.97 Forearm.reconfigure LooseForearm @14:00:41.01 Forearm.seek :retract @14:00:42.02 Flushing the Collection Valve::Manifold @14:00:42.16 CSV.dial CSR @14:00:43.58 Pulling 5ml of CSR.flush @14:00:43.59 CSR.select :flush @14:00:43.72 CS.reconfigure CSconfig @14:00:43.77 CS.seek 5ml @14:00:57.51 delay 5 seconds @14:01:02.53 CSR.select :air @14:01:02.70 CS.reconfigure CSfast @14:01:02.75 CS.seek 7.5ml @14:01:09.59 CSV.dial :waste @14:01:10.94 CS.reconfigure CSconfig @14:01:10.99 CS.seek 0ml @14:01:31.16 Purging external Intake @14:01:31.29 CSV.dial :flush @14:01:31.77 CS.reconfigure CSfast @14:01:31.82 CS.seek 10ml @14:01:55.82 delay 5 seconds @14:02:00.85 CSV.dial CBV @14:02:00.88
CBV.dial CSV @14:02:01.68 CTV.dial :intake @14:02:02.47 Intake.select :open @14:02:02.60 CS.seek 0ml @14:02:27.65 Intake.select :close @14:02:27.77 CTV.dial :air @14:02:28.55 Flushing upper manifold @14:02:28.68 CSV.dial :flush @14:02:29.46 CS.seek 5ml @14:02:41.98 delay 5 seconds @14:02:47.00 CSV.dial :air @14:02:47.67 CS.seek 7ml @14:02:53.29 CSV.dial CBV @14:02:53.31
CBV.dial CSV @14:02:54.38 CTV.dial :air @14:02:54.40 CS.seek 0ml @14:03:11.48 delay 7 seconds @14:03:18.52 CS.seek 3ml @14:03:26.53 CSV.dial :waste @14:03:27.01 CS.seek 0ml @14:03:35.00 CSV.dial :flush @14:03:35.47 CS.seek 3ml @14:03:43.95 delay 5 seconds @14:03:48.97 CSV.dial :air @14:03:49.52 CS.seek 5ml @14:03:55.19 CSV.dial :air @14:03:55.22 CS.seek 0ml @14:04:07.91 delay 7 seconds @14:04:14.95 CS.seek 3ml @14:04:22.97 CSV.dial :waste @14:04:23.77 CS.seek 0ml @14:04:31.70 Garaging FlushPuck in Collection Clamp @14:04:31.78 Elbow.reconfigure TightElbow @14:04:31.83 Elbow.seek CC @14:04:32.07 Elbow.reconfigure LooseElbow @14:04:32.18 Forearm.seek CC @14:04:33.12 Hand.close @14:04:33.71 CC.seek :open @14:04:38.04 Forearm.seek :retract @14:04:39.01 Elbow.reconfigure TightElbow @14:04:39.06 Elbow.seek :garage @14:04:42.04 Elbow.reconfigure LooseElbow @14:04:42.14 Forearm.reconfigure TightForearm @14:04:42.20 Forearm.seek :garage @14:04:43.34 Hand.open @14:04:44.02 Forearm.reconfigure LooseForearm @14:04:44.07 Forearm.seek :retract @14:04:45.15 Starting mfb @14:04:45.17 pcrBAC sampling at most 150ml, wcr at most 1500ml @14:04:45.23 BACfans.email "Started mfb in tube 3 at 14:04:45CEST11-Oct-18\n{1 => 22, 2 => 8, 3 => 14}\nCan@14:00:25, 13.4C, 70% humidity, 14.3psia, 13.294V, 0.979A, 0.979A avg, 13.01W",Subject: "pcrBAC sampling at most 150ml, wcr at most 1500ml" @14:04:45.28
Loading sh1 puck from tube 3 into Collection Clamp @14:04:45.40 Readying the MFB @14:04:45.43 Homing MFB Syringe @14:04:45.47 AFV.select :holdingCoil @14:04:45.71 ATV.dial :air2 @14:04:45.74
SC.reconfigure SCtight @14:04:45.83 SC.seek 3 @14:04:46.29 AS.reconfigure ASconfig @14:04:46.36 AS.home.seek :home @14:04:47.93
SC.reconfigure SCloose @14:04:48.15 Elbow.reconfigure TightElbow @14:04:48.20 Elbow.seek 3 @14:04:50.37 Forearm.reconfigure TightForearm @14:04:50.43 Forearm.seek 3 + 700 counts @14:04:51.45 SE.reconfigure SEpick @14:04:51.52 SE.seek beamBreak @14:04:51.89 AFV.select :carrierSolution @14:04:52.01 delay 2 seconds @14:04:53.47
SE.configure SEconfig @14:04:53.66 Elbow.seek 3 @14:04:54.10 AS.seek 1ml @14:04:54.18
Forearm.seek 3 + 300 counts @14:04:54.46 Forearm.reconfigure LooseForearm @14:04:54.50 Hand.close @14:04:55.11 SE.seek :down @14:04:55.39
Forearm.seek :retract @14:04:56.41 Elbow.seek CC @14:04:56.80 SE.coast @14:04:58.19
Elbow.reconfigure LooseElbow @14:04:58.29 Forearm.reconfigure TightForearm @14:04:58.34 Forearm.seek CC @14:05:00.08 CC.seek :closed @14:05:04.35 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.04 @14:05:04.36 Hand.open @14:05:05.03 Forearm.reconfigure LooseForearm @14:05:05.09 Forearm.seek :retract @14:05:06.07 SC.coast @14:05:06.11 collectSample 150ml w/bubble point of 55psi @14:05:06.12 Priming 7ml @14:05:06.13 Intake.select :close @14:05:06.14 Exhaust.select :close @14:05:06.16 SS.hold @14:05:06.39 Calibrating pressure and temperature sensors @14:05:06.41 CTV.dial :air @14:05:06.42 SSV.dial :spit,avoiding: :exhaust @14:05:06.44 SS.reconfigure SSfast @14:05:06.49 SS.seek 5ml @14:05:09.40 SSV.dial CBV @14:05:09.42
CBV.dial SSV @14:05:10.88 delay 3.8 seconds @14:05:14.82 SSV.dial :spit @14:05:16.21 SS.seek 0ml @14:05:18.93 SSV.dial CBV @14:05:20.32 CTV.dial :intake @14:05:21.10 SSV.dial CBV @14:05:21.13
CBV.dial SSV @14:05:21.14 Allowing 40s for 7.0ml of remaining 7.0ml @14:05:21.15 Intake.select :open @14:05:23.33 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:05:23.35 SS.reconfigure SSinhale.with maxSpeed: 150 @14:05:23.40 SS.seek 7ml @14:05:26.83 Vacuum too high -- waiting up to 40s @14:05:28.42 Intake @32.8psia, vacuum from 20.0 to 24.0psi @14:05:28.44 SS.reconfigure SSinhale.with maxSpeed: 135 @14:05:28.49 SS.seek 7ml @14:05:31.20 Vacuum too high -- waiting up to 40s @14:05:33.07 Intake @32.9psia, vacuum from 20.0 to 24.0psi @14:05:33.09 SS.reconfigure SSinhale.with maxSpeed: 122 @14:05:33.14 SS.seek 7ml @14:05:35.26 Stroke completed -- waiting for pressure to equalize ... @14:05:37.83 Intake.select :close @14:05:37.99 SSV.dial :exhaust @14:05:38.79 Exhaust.select :open @14:05:38.91 SS.reconfigure SSexhale @14:05:38.96 SS.seek 0ml @14:05:42.55 Exhaust.select :close @14:05:42.67 Sampling 150.0ml @14:05:42.68 Intake.select :close @14:05:42.69 Exhaust.select :close @14:05:42.71 SS.reconfigure SSconfig @14:05:42.76 SS.hold @14:05:42.98 CTV.dial :intake @14:05:42.99 SSV.dial CBV @14:05:43.02
CBV.dial SSV @14:05:43.85 Allowing 130s for 25.0ml of remaining 150.0ml @14:05:43.86 Intake.select :open @14:05:46.03 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:05:46.04 SS.reconfigure SSinhale.with maxSpeed: 122 @14:05:46.60 SS.seek 25ml @14:05:47.02 delay 1 second @14:05:48.03 AFV.select :holdingCoil @14:05:48.14 delay 1 second @14:05:49.16 ATV.dial :waste @14:05:50.16 AS.reconfigure ASnorm @14:05:50.21 AS.home.seek :empty @14:05:50.49
Vacuum too high -- waiting up to 40s @14:05:52.12 Intake @32.9psia, vacuum from 20.0 to 24.0psi @14:05:52.14 SS.reconfigure SSinhale.with maxSpeed: 110 @14:05:52.19 SS.seek 25ml @14:05:55.02 Vacuum too high -- waiting up to 40s @14:05:56.66 SS.reconfigure SSinhale.with maxSpeed: 99 @14:05:56.71 SS.seek 25ml @14:05:59.17 Vacuum too high -- waiting up to 40s @14:06:00.79 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:06:00.81 SS.reconfigure SSinhale.with maxSpeed: 89 @14:06:00.86 SS.seek 25ml @14:06:03.49 Vacuum too high -- waiting up to 40s @14:06:04.82 Intake @32.9psia, vacuum from 20.0 to 24.0psi @14:06:04.84 SS.reconfigure SSinhale.with maxSpeed: 80 @14:06:04.89 SS.seek 25ml @14:06:07.45 Vacuum too high -- waiting up to 40s @14:06:08.80 Intake @32.8psia, vacuum from 20.0 to 24.0psi @14:06:08.82 SS.reconfigure SSinhale.with maxSpeed: 72 @14:06:08.87 SS.seek 25ml @14:06:11.93 Vacuum too high -- waiting up to 40s @14:06:13.27 Intake @32.9psia, vacuum from 20.0 to 24.0psi @14:06:13.29 SS.reconfigure SSinhale.with maxSpeed: 65 @14:06:13.34 SS.seek 25ml @14:06:16.86 Vacuum too high -- waiting up to 40s @14:06:18.22 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:06:18.24 SS.reconfigure SSinhale.with maxSpeed: 59 @14:06:18.29 SS.seek 25ml @14:06:22.34 Vacuum too high -- waiting up to 40s @14:06:23.69 Intake @32.8psia, vacuum from 20.0 to 24.0psi @14:06:24.21 SS.reconfigure SSinhale.with maxSpeed: 53 @14:06:24.33 SS.seek 25ml @14:06:30.96 Stroke completed -- waiting for pressure to equalize ... @14:06:34.21 Intake.select :close @14:06:34.37 SSV.dial :exhaust @14:06:35.18 Exhaust.select :open @14:06:35.29 SS.reconfigure SSexhale @14:06:35.34 SS.seek 0ml @14:06:42.79 ATV.dial :waste @14:06:42.81 ARV.dial between carrier and air1 @14:06:42.90 ASV.dial :waste1 @14:06:44.37
Exhaust.select :close @14:06:44.49 SSV.dial CBV @14:06:44.52
CBV.dial SSV @14:06:45.35 Allowing 130s for 25.0ml of remaining 125.0ml @14:06:45.36 Intake.select :open @14:06:47.53 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:06:47.55 SS.reconfigure SSinhale.with maxSpeed: 53 @14:06:47.60 SS.seek 25ml @14:06:54.05 Vacuum too high -- waiting up to 40s @14:06:55.13 Intake @32.9psia, vacuum from 20.0 to 24.0psi @14:06:55.15 SS.reconfigure SSinhale.with maxSpeed: 48 @14:06:55.20 SS.seek 25ml @14:07:04.77 Vacuum too high -- waiting up to 40s @14:07:05.85 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:07:05.86 SS.reconfigure SSinhale.with maxSpeed: 43 @14:07:05.91 SS.seek 25ml @14:07:29.71 Stroke completed -- waiting for pressure to equalize ... @14:07:32.90 Intake.select :close @14:07:33.05 SSV.dial :exhaust @14:07:33.86 Exhaust.select :open @14:07:33.98 SS.reconfigure SSexhale @14:07:34.03 SS.seek 0ml @14:07:42.75 Exhaust.select :close @14:07:42.87 SSV.dial CBV @14:07:42.90
CBV.dial SSV @14:07:43.74 Allowing 130s for 25.0ml of remaining 100.0ml @14:07:43.75 Intake.select :open @14:07:45.92 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:07:45.94 SS.reconfigure SSinhale.with maxSpeed: 43 @14:07:45.99 SS.seek 25ml @14:08:11.49 Vacuum too high -- waiting up to 40s @14:08:12.56 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:08:12.57 SS.reconfigure SSinhale.with maxSpeed: 39 @14:08:12.62 SS.seek 25ml @14:08:30.92 Stroke completed -- waiting for pressure to equalize ... @14:08:34.12 Intake.select :close @14:08:34.27 SSV.dial :exhaust @14:08:35.09 Exhaust.select :open @14:08:35.21 SS.reconfigure SSexhale @14:08:35.26 SS.seek 0ml @14:08:43.93 Exhaust.select :close @14:08:44.05 SSV.dial CBV @14:08:44.08
CBV.dial SSV @14:08:44.91 Allowing 130s for 25.0ml of remaining 75.0ml @14:08:44.92 Intake.select :open @14:08:47.09 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:08:47.11 SS.reconfigure SSinhale.with maxSpeed: 39 @14:08:47.16 SS.seek 25ml @14:09:33.01 Stroke completed -- waiting for pressure to equalize ... @14:09:36.10 Intake.select :close @14:09:36.26 SSV.dial :exhaust @14:09:37.07 Exhaust.select :open @14:09:37.18 SS.reconfigure SSexhale @14:09:37.23 SS.seek 0ml @14:09:46.20 Exhaust.select :close @14:09:46.32 SSV.dial CBV @14:09:46.35
CBV.dial SSV @14:09:47.19 Allowing 130s for 25.0ml of remaining 50.0ml @14:09:47.20 Intake.select :open @14:09:49.37 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:09:49.39 SS.reconfigure SSinhale.with maxSpeed: 39 @14:09:49.44 SS.seek 25ml @14:10:34.67 Stroke completed -- waiting for pressure to equalize ... @14:10:37.90 Intake.select :close @14:10:38.06 SSV.dial :exhaust @14:10:38.86 Exhaust.select :open @14:10:38.98 SS.reconfigure SSexhale @14:10:39.03 SS.seek 0ml @14:10:48.07 Exhaust.select :close @14:10:48.19 SSV.dial CBV @14:10:48.22
CBV.dial SSV @14:10:49.04 Allowing 130s for 25.0ml of remaining 25.0ml @14:10:49.05 Intake.select :open @14:10:51.22 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:10:51.24 SS.reconfigure SSinhale.with maxSpeed: 39 @14:10:51.29 SS.seek 25ml @14:11:36.53 Stroke completed -- waiting for pressure to equalize ... @14:11:40.37 Intake.select :close @14:11:40.52 SSV.dial :exhaust @14:11:41.32 Exhaust.select :open @14:11:41.44 SS.reconfigure SSexhale @14:11:41.50 SS.seek 0ml @14:11:50.60 Exhaust.select :close @14:11:50.72 Sampled 150.0ml @14:11:50.74 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @14:11:50.81 CSV.dial :waste @14:11:50.87 CS.seek 0ml @14:11:50.99 CSV.dial :air @14:11:51.02 SSV.dial CBV,avoiding: :exhaust @14:11:51.07
CBV.dial SSV @14:11:51.86 CS.seek 10ml @14:11:53.07
CTV.dial :air @14:11:53.86 delay 15 seconds @14:12:08.89 SSV.dial :spit,avoiding: :exhaust @14:12:10.29 CTV.dial CSV,avoiding: :intake @14:12:10.34
SS.seek 0ml @14:12:10.48 SSV.dial CBV,avoiding: :exhaust @14:12:16.20 CBV.dial between SSV and waste2 @14:12:16.62 CTV.dial CSV,avoiding: :air @14:12:16.65
CSV.dial CTV @14:12:17.11 CS.reconfigure CSpressurize @14:12:17.16 CS.seek 0ml @14:12:43.25 BubblePoint: Intake @69.7psia, Outlet @14.4psia @14:12:43.26 CBV.dial SSV @14:12:43.61 SS.reconfigure SSconfig @14:12:43.66 SS.seek 10ml @14:12:49.32 delay 15 seconds @14:13:04.45 Evacuated: Intake @35.7psia, Outlet @1.0psia @14:13:04.46 SSV.dial :spit,avoiding: :exhaust @14:13:05.85 SS.reconfigure SSexhale @14:13:05.90 SS.seek 0ml @14:13:11.00 SS.reconfigure SSfast @14:13:11.05 SS.seek 5ml @14:13:13.77 SSV.dial CBV,avoiding: :exhaust @14:13:13.80
CBV.dial SSV @14:13:15.19 CTV.dial :air,avoiding: :intake @14:13:16.56 CSV.dial CTV @14:13:16.60
SS.seek 10ml @14:13:17.59 CSV.dial :air @14:13:18.56 CS.reconfigure CSfast @14:13:18.61 CS.seek 0ml @14:13:19.04 CS.coast @14:13:19.27
SSV.dial :spit,avoiding: :exhaust @14:13:20.66 SS.reconfigure SSexhale @14:13:20.71 SS.seek 0ml @14:13:25.72 SS.reconfigure SSfast @14:13:25.77 SS.seek 5ml @14:13:28.43 SSV.dial CBV,avoiding: :exhaust @14:13:28.46
CBV.dial SSV @14:13:30.88 SSV.dial :spit,avoiding: :exhaust @14:13:32.29 SS.reconfigure SSconfig @14:13:32.34 SS.seek 0ml @14:13:35.43 SS.coast @14:13:35.48 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @14:13:35.49 Intake.select :close @14:13:35.57 CSV.dial :waste @14:13:35.70 SSV.dial CBV,avoiding: :exhaust @14:13:35.73
CBV.dial SSV @14:13:36.40 CS.seek 0ml @14:13:36.48 CSV.dial :air @14:13:37.13
CTV.dial :air @14:13:37.14 delay 15 seconds @14:13:37.27 CS.seek 10ml @14:13:52.17
SSV.dial :spit,avoiding: :exhaust @14:13:53.55 CTV.dial CSV,avoiding: :intake @14:13:53.59
SS.reconfigure SSexhale @14:13:53.70 SS.seek 0ml @14:13:53.76 SSV.dial CBV,avoiding: :exhaust @14:14:01.39 CBV.dial between SSV and waste2 @14:14:01.82 CSV.dial between air and CTV @14:14:02.15 CS.seek 8.288ml @14:14:07.27 CTV.dial CSV,avoiding: :air @14:14:07.30
CSV.dial CTV @14:14:07.63 CS.reconfigure CSpressurize @14:14:07.68 CS.seek 0ml @14:14:29.51 CSV.dial :air @14:14:30.13 CS.reconfigure CSfast @14:14:30.18 CS.seek 10ml @14:14:54.08 CSV.dial between air and CTV @14:14:54.42 CS.seek 3.132ml @14:15:11.39 CTV.dial CSV,avoiding: :air @14:15:11.42
CSV.dial CTV @14:15:11.86 CS.reconfigure CSpressurize @14:15:11.91 CS.seek 0ml @14:15:20.19 BubblePoint: Intake @69.1psia, Outlet @14.0psia @14:15:20.20 CBV.dial SSV @14:15:20.55 SS.reconfigure SSconfig @14:15:20.60 SS.seek 10ml @14:15:25.97 delay 15 seconds @14:15:41.10 Evacuated: Intake @35.6psia, Outlet @17.6psia @14:15:41.11 SSV.dial :spit,avoiding: :exhaust @14:15:42.51 SS.reconfigure SSexhale @14:15:42.56 SS.seek 0ml @14:15:47.75 SS.reconfigure SSfast @14:15:47.80 SS.seek 5ml @14:15:50.35 SSV.dial CBV,avoiding: :exhaust @14:15:50.38
CBV.dial SSV @14:15:51.79 CTV.dial :air,avoiding: :intake @14:15:53.16 CSV.dial CTV @14:15:53.21
SS.seek 10ml @14:15:54.20 CSV.dial :air @14:15:54.68 CS.reconfigure CSfast @14:15:54.73 CS.seek 0ml @14:15:55.82 CS.coast @14:15:55.94
SSV.dial :spit,avoiding: :exhaust @14:15:57.33 SS.reconfigure SSexhale @14:15:57.38 SS.seek 0ml @14:16:02.37 SS.reconfigure SSfast @14:16:02.42 SS.seek 5ml @14:16:05.07 SSV.dial CBV,avoiding: :exhaust @14:16:05.10
CBV.dial SSV @14:16:07.50 SSV.dial :spit,avoiding: :exhaust @14:16:09.41 SS.reconfigure SSconfig @14:16:09.46 SS.seek 0ml @14:16:12.82 SS.coast @14:16:12.87 Lysing: Adding 1.5ml lysis @14:16:12.88 CBV.dial between CSV and waste1,avoiding: :DBV @14:16:14.44 CTV.dial CSV @14:16:15.83 CSV.dial CSR @14:16:16.67 Pulling 1ml of CSR.lysis @14:16:16.68 CSR.select :lysis @14:16:16.79 CS.reconfigure CSconfig @14:16:16.84 CS.seek 1ml @14:16:20.43 delay 5 seconds @14:16:25.45 CSR.select :air @14:16:25.63 CS.seek 6ml @14:16:39.49 delay 2 seconds @14:16:41.50 CSV.dial :waste1 @14:16:42.86 CS.reconfigure CSfast @14:16:42.91 CS.seek :empty @14:16:57.73 CSV.dial CSR @14:16:59.16 Pulling 1.5ml of CSR.lysis @14:16:59.17 CSR.select :lysis @14:16:59.30 CS.reconfigure CSconfig @14:16:59.35 CS.seek 1.5ml @14:17:04.21 delay 5 seconds @14:17:09.23 CSR.select :air @14:17:09.41 CS.seek 4.5ml @14:17:18.08 delay 2 seconds @14:17:20.09 CSV.dial CTV @14:17:20.59 CS.seek 1.275ml @14:17:29.86 Heating sh1 puck from tube 3 in Collection Clamp to 85C for 5:00 @14:17:29.88 CH.seek 85C,8:00 @14:20:21.43 CH.hold 5:00,85C @14:25:21.60 Collection Puck Heater at 85.1C after lysing @14:25:21.62 CS.seek 0ml @14:25:25.84 CTV.dial between spare and air,avoiding: :intake @14:25:26.94 CSV.dial :air @14:25:27.54 CS.seek 5ml @14:25:41.36 CSV.dial CTV @14:25:41.82 delay 2 seconds @14:25:43.83 CSV.dial :air @14:25:44.31 delay 2 seconds @14:25:46.33 CSV.dial CTV @14:25:47.34 delay 2 seconds @14:25:49.35 CSV.dial :air @14:25:49.84 delay 2 seconds @14:25:51.87 CS.seek 0ml @14:26:05.59 Recovering Lysate into Collection syringe @14:26:05.60 CSV.dial CBV @14:26:06.67 CBV.dial CSV @14:26:07.08 CS.seek 5ml @14:26:20.90 delay 5 seconds @14:26:25.92 CTV.dial :air @14:26:26.49 CS.seek 7.001ml @14:26:33.08 delay 5 seconds @14:26:38.10 CSV.dial :air2 @14:26:38.59 CS.seek 10ml @14:26:47.22 CSV.dial between air and CTV,avoiding: CBV @14:26:48.42 Lysing Complete @14:26:48.50 Storing sh1 puck in Collection Clamp to tube 2 @14:26:48.70 SC.reconfigure SCtight @14:26:48.74 SC.seek 2 @14:26:50.86 SC.reconfigure SCloose @14:26:51.09 Elbow.reconfigure TightElbow @14:26:51.14 Elbow.seek 2 @14:26:53.03 Forearm.reconfigure TightForearm @14:26:53.08 Forearm.seek 2 + 700 counts @14:26:54.16 SE.reconfigure SEpick @14:26:54.24 SE.seek beamBreak @14:26:56.58 SE.configure SEconfig @14:26:56.75 Elbow.reconfigure LimpElbow @14:26:56.80 Forearm.reconfigure LooseForearm @14:26:56.84 Forearm.seek 2 @14:26:57.14 Elbow.hold @14:26:57.21 Elbow.reconfigure TightElbow @14:26:57.31 SE.seek 7.08 Pucks below Top Plate @14:26:57.91 Forearm.force -20 @14:26:57.95 Forearm.seek 2 + 345 counts @14:26:58.28 Forearm.reconfigure TightForearm @14:26:58.33 Forearm.seek 2 + 700 counts @14:26:58.54 SE.seek 8.08 Pucks below Top Plate @14:26:59.26 Forearm.reconfigure LooseForearm @14:26:59.30 Forearm.seek :retract @14:27:00.26 Elbow.seek CC @14:27:02.01 Elbow.reconfigure LooseElbow @14:27:02.12 Forearm.seek CC @14:27:03.08 Hand.close @14:27:03.74 CC.seek :open @14:27:08.23 Forearm.seek :retract @14:27:09.18 Elbow.reconfigure TightElbow @14:27:09.23 Elbow.seek 2 @14:27:11.10 Elbow.reconfigure LimpElbow @14:27:11.16 Forearm.seek 2 @14:27:12.67 Elbow.hold @14:27:12.74 Elbow.reconfigure TightElbow @14:27:12.79 Hand.open @14:27:13.51 Forearm.force -20 @14:27:13.56 Forearm.seek 2 + 342 counts @14:27:13.89 Forearm.reconfigure TightForearm @14:27:13.94 Forearm.seek 2 + 700 counts @14:27:14.33 SE.seek :down @14:27:15.49
Forearm.reconfigure LooseForearm @14:27:15.54 Forearm.seek :retract @14:27:16.30 SE.coast @14:27:16.50
SC.coast @14:27:16.54 Tranferring lysate to PRV @14:27:16.55 CSV.dial :air @14:27:16.88 CS.seek 10ml @14:27:16.97 CSV.dial between air and CTV @14:27:17.33 AIV.dial :bypass @14:27:17.43 Beginning samplePassC @14:27:17.44 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:27:17.52 Hand.open @14:27:17.89 Elbow.seek FlushPuck::Garage @14:27:19.91 Elbow.reconfigure LooseElbow @14:27:20.02 Forearm.seek :garage @14:27:21.00 Hand.close @14:27:21.75 Forearm.seek :retract @14:27:22.78 Elbow.reconfigure TightElbow @14:27:22.83 Elbow.seek CC @14:27:25.74 Elbow.reconfigure LooseElbow @14:27:25.84 Forearm.reconfigure TightForearm @14:27:25.89 Forearm.seek CC @14:27:27.14 CC.seek :closed @14:27:31.39 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.05 @14:27:31.47 Hand.open @14:27:32.18 Forearm.reconfigure LooseForearm @14:27:32.23 Forearm.seek :retract @14:27:33.17 AWV.select :air @14:27:33.19 AIV.dial :bypass @14:27:33.20 PSV.dial PSR @14:27:34.74 Pulling 1.5ml of PSR.flush @14:27:34.75 PSR.select :flush @14:27:34.88 PS.seek 1.5ml @14:27:50.89 delay 5 seconds @14:27:55.91 PSV.dial :waste1 @14:27:56.40 PS.seek 0ml @14:28:12.47 PSV.dial PSR @14:28:12.95 PSR.select :air @14:28:13.08 PS.seek 2.5ml @14:28:39.23 PRV.dial CBV @14:28:39.26
CBV.dial PRV @14:28:40.66 PSV.dial :mixloop @14:28:41.72 CTV.dial :air,avoiding: :intake @14:28:41.73 PTV.dial :PRVmixing @14:28:41.84 PS.seek 0ml @14:29:07.97 PSV.dial :air @14:29:08.46 PS.seek 2.5ml @14:29:34.60 PSV.dial :mixloop @14:29:35.09 PS.seek 0ml @14:30:01.22 PSV.dial :air @14:30:01.70 PS.seek 2.5ml @14:30:27.82 PSV.dial :mixloop @14:30:28.31 PS.seek 0ml @14:30:54.45 Garaging FlushPuck in Collection Clamp @14:30:54.53 Elbow.reconfigure TightElbow @14:30:54.58 Elbow.seek CC @14:30:54.86 Elbow.reconfigure LooseElbow @14:30:54.97 Forearm.seek CC @14:30:55.93 Hand.close @14:30:56.50 CC.seek :open @14:31:00.88 Forearm.seek :retract @14:31:01.86 Elbow.reconfigure TightElbow @14:31:01.91 Elbow.seek :garage @14:31:04.98 Elbow.reconfigure LooseElbow @14:31:05.10 Forearm.reconfigure TightForearm @14:31:05.15 Forearm.seek :garage @14:31:06.32 Hand.open @14:31:07.07 Forearm.reconfigure LooseForearm @14:31:07.12 Forearm.seek :retract @14:31:08.16 Connecting PS to CS to transfer sample @14:31:08.17 CBV.dial AIV @14:31:08.18 AIV.dial :bypass @14:31:08.19 PSV.dial :mixloop @14:31:08.21 PTV.dial :PRVmixing @14:31:08.22 PRV.dial AIV @14:31:08.23 CTV.dial :air,avoiding: :intake @14:31:08.25 Loading lyfil puck from tube 3 into Collection Clamp @14:31:08.51 SC.reconfigure SCtight @14:31:08.56 SC.seek 3 @14:31:10.72 SC.reconfigure SCloose @14:31:10.95 Elbow.reconfigure TightElbow @14:31:11.00 Elbow.seek 3 @14:31:13.05 Forearm.reconfigure TightForearm @14:31:13.10 Forearm.seek 3 + 700 counts @14:31:14.13 SE.reconfigure SEpick @14:31:14.20 SE.seek beamBreak @14:31:16.15 SE.configure SEconfig @14:31:16.31 Elbow.seek 3 @14:31:16.65 Forearm.seek 3 + 300 counts @14:31:16.88 Forearm.reconfigure LooseForearm @14:31:16.92 Hand.close @14:31:17.51 SE.seek :down @14:31:17.75
Forearm.seek :retract @14:31:18.69 Elbow.seek CC @14:31:19.19 SE.coast @14:31:20.42
Elbow.reconfigure LooseElbow @14:31:21.00 Forearm.reconfigure TightForearm @14:31:21.08 Forearm.seek CC @14:31:22.38 CC.seek :closed @14:31:26.65 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.06 @14:31:26.67 Hand.open @14:31:27.32 Forearm.reconfigure LooseForearm @14:31:27.37 Forearm.seek :retract @14:31:28.35 SC.coast @14:31:28.38 CTV.dial CSV,avoiding: :intake @14:31:29.78 CSV.dial CTV @14:31:30.11 CS.seek 5ml @14:31:43.81 delay 1:20 @14:33:03.84 CS.seek 2.5ml @14:33:11.32 delay 40 seconds @14:33:51.35 CS.seek 0ml @14:33:58.72 delay 1:00 @14:34:58.74 PSV.dial :air @14:34:59.22 PS.seek 2.5ml @14:35:25.32 PSV.dial :mixloop @14:35:25.80 delay 3 seconds @14:35:28.82 PSV.dial :air @14:35:29.30 delay 2 seconds @14:35:31.31 PSV.dial :mixloop @14:35:31.80 delay 3 seconds @14:35:34.81 PSV.dial :waste1 @14:35:36.19 PS.seek :empty @14:36:02.29 CBV.dial :waste1 @14:36:02.79 delay 5 seconds @14:36:07.81 CBV.dial AIV @14:36:08.32 delay 10 seconds @14:36:18.34 CBV.dial :waste1 @14:36:18.83 delay 10 seconds @14:36:28.85 CBV.dial AIV @14:36:29.35 delay 10 seconds @14:36:39.38 CTV.dial CSV,avoiding: :intake @14:36:39.39 CBV.dial between AIV and waste1 @14:36:39.74 CSV.dial :air @14:36:40.23 CS.seek 10ml @14:37:06.75 CSV.dial CTV @14:37:07.24 CS.seek 0ml @14:37:33.75 CBV.dial :waste1 @14:37:34.08 delay 10 seconds @14:37:44.11 CTV.dial :air,avoiding: :intake @14:37:45.55 Storing lyfil puck in Collection Clamp to tube 2 @14:37:45.73 SC.reconfigure SCtight @14:37:45.78 SC.seek 2 @14:37:47.90 SC.reconfigure SCloose @14:37:48.13 Elbow.reconfigure TightElbow @14:37:48.17 Elbow.seek 2 @14:37:50.09 Forearm.reconfigure TightForearm @14:37:50.14 Forearm.seek 2 + 700 counts @14:37:51.24 SE.reconfigure SEpick @14:37:51.32 SE.seek beamBreak @14:37:53.68 SE.configure SEconfig @14:37:53.85 Elbow.reconfigure LimpElbow @14:37:53.90 Forearm.reconfigure LooseForearm @14:37:53.95 Forearm.seek 2 @14:37:54.26 Elbow.hold @14:37:54.33 Elbow.reconfigure TightElbow @14:37:54.43 SE.seek 8.09 Pucks below Top Plate @14:37:55.44 Forearm.force -20 @14:37:55.56 Forearm.seek 2 + 338 counts @14:37:55.89 Forearm.reconfigure TightForearm @14:37:55.94 Forearm.seek 2 + 700 counts @14:37:56.18 SE.seek 9.09 Pucks below Top Plate @14:37:56.83 Forearm.reconfigure LooseForearm @14:37:56.88 Forearm.seek :retract @14:37:57.82 Elbow.seek CC @14:37:59.40 Elbow.reconfigure LooseElbow @14:37:59.51 Forearm.seek CC @14:38:00.50 Hand.close @14:38:01.14 CC.seek :open @14:38:05.64 Forearm.seek :retract @14:38:06.60 Elbow.reconfigure TightElbow @14:38:06.65 Elbow.seek 2 @14:38:08.52 Elbow.reconfigure LimpElbow @14:38:08.57 Forearm.seek 2 @14:38:09.60 Elbow.hold @14:38:09.67 Elbow.reconfigure TightElbow @14:38:09.72 Hand.open @14:38:10.53 Forearm.force -20 @14:38:10.57 Forearm.seek 2 + 348 counts @14:38:10.90 Forearm.reconfigure TightForearm @14:38:10.95 Forearm.seek 2 + 700 counts @14:38:11.36 SE.seek :down @14:38:12.52
Forearm.reconfigure LooseForearm @14:38:12.57 Forearm.seek :retract @14:38:13.31 SE.coast @14:38:13.50
SC.coast @14:38:13.53 PSV.dial :air @14:38:14.67 PS.seek 0.499ml @14:38:20.52 delay 3 seconds @14:38:23.53 PSV.dial :mixloop @14:38:24.00 PRV.dial :air @14:38:24.47 delay 3 seconds @14:38:27.49 PRV.dial CBV @14:38:27.97 CBV.dial AIV @14:38:28.46 PRV.dial between CBV and air @14:38:28.81 PRV.dial :air @14:38:29.19 PS.reconfigure PSslow @14:38:29.24 PS.seek 0.599ml @14:38:31.76 PRV.dial :waste2 @14:38:32.52 PS.reconfigure PSconfig @14:38:32.57 PS.seek :empty @14:38:39.46 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:38:39.53 Hand.open @14:38:39.89 Elbow.seek FlushPuck::Garage @14:38:41.88 Elbow.reconfigure LooseElbow @14:38:41.99 Forearm.seek :garage @14:38:42.97 Hand.close @14:38:43.77 Forearm.seek :retract @14:38:45.31 Elbow.reconfigure TightElbow @14:38:45.36 Elbow.seek CC @14:38:48.20 Elbow.reconfigure LooseElbow @14:38:48.30 Forearm.reconfigure TightForearm @14:38:48.35 Forearm.seek CC @14:38:49.61 CC.seek :closed @14:38:53.87 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.07 @14:38:53.95 Hand.open @14:38:54.71 Forearm.reconfigure LooseForearm @14:38:54.75 Forearm.seek :retract @14:38:55.83 Clearing PML for modified lysate @14:38:55.84 CTV.dial :air,avoiding: :intake @14:38:55.85 PRV.dial :waste2 @14:38:55.87 PTV.dial :PRV,avoiding: :puck @14:38:56.64 delay 2 seconds @14:38:58.66 PTV.dial :PRVmixing,avoiding: :puck @14:38:59.44 delay 2 seconds @14:39:01.45 PSV.dial :PML @14:39:01.47 delay 2 seconds @14:39:03.49 PRV.dial :air @14:39:04.27 delay 2 seconds @14:39:06.35 PS.seek 1.499ml @14:39:22.44 delay 5 seconds @14:39:27.47 PS.seek 2.5ml @14:39:38.46 PSV.dial :air @14:39:38.94 PS.seek 0ml @14:40:05.02 mixing lysate with speDil @14:40:05.03 PSV.dial :PML @14:40:05.49 CBV.dial :PRV @14:40:05.51 PRV.dial :air @14:40:05.52 delay 2 seconds @14:40:07.55 PRV.dial :speDil @14:40:08.04 delay 2 seconds @14:40:10.12 PS.reconfigure PSslow1 @14:40:10.17 PS.seek 0.225ml @14:40:33.90 delay 5 seconds @14:40:38.93 PRV.dial CBV @14:40:39.72 delay 2 seconds @14:40:41.80 PS.seek 0.475ml @14:41:07.83 delay 5 seconds @14:41:12.86 PRV.dial :air @14:41:13.36 delay 2 seconds @14:41:15.39 PS.seek 0.965ml @14:42:05.37 delay 10 seconds @14:42:15.39 PRV.dial CBV @14:42:15.90 CTV.dial :air,avoiding: :intake @14:42:15.91 PSV.dial :air @14:42:16.40 PS.reconfigure PSconfig @14:42:16.45 PS.seek 2.5ml @14:42:32.83 delay 3 seconds @14:42:35.85 PSV.dial PTV @14:42:36.62 PTV.dial :PRV,avoiding: :puck @14:42:37.41 PS.seek 0ml @14:43:03.51 PSV.dial :air @14:43:04.29 PS.seek 2.5ml @14:43:30.43 delay 3 seconds @14:43:33.45 PSV.dial PTV @14:43:34.23 PTV.dial :PRV,avoiding: :puck @14:43:34.25 PS.seek 0ml @14:44:00.37 Pushing modified lysate into AIV Injection Loop @14:44:00.38 CBV.dial AIV @14:44:00.39 AIV.dial :bypass @14:44:00.40 PRV.dial CBV @14:44:00.42 PSV.dial :air @14:44:01.19 PS.seek 2.5ml @14:44:27.90 delay 3 seconds @14:44:30.91 PSV.dial :PML @14:44:31.40 PTV.dial :PRVmixing,avoiding: :puck @14:44:32.25 PS.reconfigure PSslow1 @14:44:32.30 PS.seek 1.74ml @14:45:49.90 delay 5 seconds @14:45:54.93 AIV.dial :sample @14:45:55.53 AWV.select :waste @14:45:55.70 PS.reconfigure PSslow2 @14:45:55.75 PS.seek 1.22ml @14:47:43.74 delay 15 seconds @14:47:58.77 AIV.dial :bypass @14:47:59.36 PS.reconfigure PSconfig @14:47:59.41 PS.seek 0ml @14:48:12.55 PSV.dial :air @14:48:13.05 PS.seek 2.5ml @14:48:39.11 PSV.dial :PML @14:48:39.59 PS.seek 0ml @14:49:05.70 AIV.dial :coilToMFB @14:49:05.72 AWV.select :air @14:49:05.88 Extracting Nucleic Acid from AIV @14:49:05.91
Beginning samplePassD @14:49:05.98 SPE.hold 2:46:40,55C @14:49:06.03 ASV.dial :SPEdelivery @14:49:06.12
AWV.select :air @14:49:06.13 AIV.dial :bypass @14:49:06.15 PSV.dial PSR @14:49:06.46 delay 20 seconds @14:49:07.32
Pulling 1.5ml of PSR.killMFB @14:49:07.33 PSR.select :killMFB @14:49:07.46 PS.seek 1.5ml @14:49:23.56 delay 5 seconds @14:49:26.49 primeLysateINT @14:49:26.50 AWV.select :air @14:49:26.51 AIV.dial :coilToMFB @14:49:26.53 ATV.dial AIV @14:49:28.23 AS.reconfigure ASslow1 @14:49:28.28 AS.seek 0.299ml @14:49:28.58
PSV.dial :waste1 @14:49:29.10 PS.seek 0ml @14:49:45.17 PSV.dial PSR @14:49:45.66 PSR.select :air @14:49:45.79 PS.seek 2.5ml @14:50:12.03 PRV.dial CBV @14:50:12.06
CBV.dial PRV @14:50:12.07 PSV.dial :mixloop @14:50:13.18 CTV.dial :air,avoiding: :intake @14:50:13.19 PTV.dial :PRVmixing @14:50:13.21 PS.seek 0ml @14:50:28.91 delay 10 seconds @14:50:38.93 ATV.dial :air2 @14:50:39.42
PSV.dial PSR @14:50:39.76 AS.seek 0.329ml @14:50:40.59
Pulling 1.5ml of PSR.flush @14:50:40.60 PSR.select :flush @14:50:40.73 PS.seek 1.5ml @14:50:46.07 ATV.dial :waste2 @14:50:46.61 AS.reconfigure ASnorm @14:50:46.67 AS.seek :empty @14:50:56.78
delay 5 seconds @14:51:01.81 PSV.dial :waste1 @14:51:02.30 PS.seek 0ml @14:51:03.68 Lysate primed! @14:51:03.69 AFV.select :holdingCoil @14:51:03.70 ATV.dial :air2 @14:51:04.33 AS.reconfigure ASslow1 @14:51:04.38 AS.seek 0.069ml @14:51:18.33
PSV.dial PSR @14:51:18.80 delay 3 seconds @14:51:18.84
PSR.select :air @14:51:18.97 PS.seek 2.5ml @14:51:21.83 ATV.dial :AIV @14:51:22.68 AS.seek 0.469ml @14:51:45.12 PRV.dial CBV @14:51:45.15
CBV.dial PRV @14:51:45.16 PSV.dial :mixloop @14:51:46.28 CTV.dial :air,avoiding: :intake @14:51:46.29 PTV.dial :PRVmixing @14:51:46.82 PS.seek 0ml @14:52:12.99 PSV.dial PSR @14:52:14.17 Pulling 1.5ml of PSR.flush @14:52:14.18 PSR.select :flush @14:52:14.31 PS.seek 1.5ml @14:52:30.31 delay 5 seconds @14:52:35.34 PSV.dial :waste1 @14:52:35.83 PS.seek 0ml @14:52:43.37 delay 5 seconds @14:52:48.46 Solid Phase Extraction Heater at 48.3C @14:52:48.47 ATV.dial ASV @14:52:48.85 ASV.dial :SPEdelivery @14:52:48.94 AS.seek 0.041ml @14:52:51.89
PSV.dial PSR @14:52:52.39 PSR.select :air @14:52:52.52 PS.seek 2.5ml @14:53:18.62 PRV.dial CBV @14:53:18.65
CBV.dial PRV @14:53:18.66 PSV.dial :mixloop @14:53:19.75 CTV.dial :air,avoiding: :intake @14:53:19.76 PTV.dial :PRVmixing @14:53:19.79 PS.seek 0ml @14:53:46.05 PSV.dial PSR @14:53:47.20 Pulling 1.5ml of PSR.flush @14:53:47.21 PSR.select :flush @14:53:47.34 PS.seek 1.5ml @14:54:03.34 delay 5 seconds @14:54:08.36 PSV.dial :waste1 @14:54:08.86 PS.seek 0ml @14:54:15.29 delay 10 seconds @14:54:24.89
PSV.dial PSR @14:54:25.31 rinseLysateLineINT @14:54:25.32 AFV.select :carrierSolution @14:54:25.38
PSR.select :air @14:54:25.45 delay 2 seconds @14:54:25.51
PS.seek 2.5ml @14:54:27.48 AS.reconfigure ASconfig @14:54:27.53 AS.seek 1ml @14:54:51.72 PRV.dial CBV @14:54:51.75
CBV.dial PRV @14:54:51.76 PSV.dial :mixloop @14:54:52.84 CTV.dial :air,avoiding: :intake @14:54:52.85 PTV.dial :PRVmixing @14:54:52.87 PS.reconfigure PSslow @14:54:52.92 PS.seek 1.2ml @14:55:19.72 PSV.dial :waste1 @14:55:21.11 PS.reconfigure PSconfig @14:55:21.16 PS.seek 0ml @14:55:22.91 delay 1 second @14:55:23.92 AFV.select :holdingCoil @14:55:24.03 delay 1 second @14:55:25.05 ATV.dial AIV @14:55:25.59 AIV.dial :coilToMFB @14:55:25.60 AWV.select :waste @14:55:25.71 AS.reconfigure ASslow0 @14:55:25.76 AS.seek 0ml @14:55:34.15
PSV.dial :air @14:55:35.37 PS.seek 2.5ml @14:56:01.52 PSV.dial :mixloop @14:56:02.00 PS.reconfigure PSslow @14:56:02.05 PS.seek 0ml @14:56:53.18 PSV.dial :air @14:56:53.67 PS.reconfigure PSconfig @14:56:53.72 PS.seek 2.5ml @14:57:06.62 ATV.dial :air2 @14:57:07.28 AS.reconfigure ASslow1 @14:57:07.33 AS.seek 0.05ml @14:57:17.76 ATV.dial :AIV @14:57:18.66 AS.seek 0.01ml @14:57:19.80
PSV.dial :mixloop @14:57:20.28 PS.reconfigure PSslow @14:57:20.33 PS.seek 0ml @14:57:26.95 ATV.dial :waste @14:57:28.57 AS.seek :empty @14:57:31.13 ethanol wash @14:57:31.14 ATV.dial :air2 @14:57:32.32 AS.reconfigure ASslow2 @14:57:32.38 AS.seek 0.009ml @14:57:42.74 delay 3 seconds @14:57:45.77 Pulling 0.4ml of Ethanol Wash @14:57:45.78 ATV.dial :ethanol @14:57:47.11 AS.reconfigure ASslow1 @14:57:47.16 AS.seek 0.409ml @14:58:11.38
Cleaning Collection Syringe @14:58:11.39 CSV.dial CSR @14:58:11.91 Pulling 4ml of CSR.flush @14:58:11.92 CSR.select :flush @14:58:12.05 CS.seek 4ml @14:58:23.46 delay 5 seconds @14:58:28.48 CSR.select :air @14:58:28.62 CS.seek 10ml @14:58:45.03 CSV.dial CTV @14:58:45.06
CTV.dial CSV @14:58:46.51 CBV.dial :waste1 @14:58:47.04 CS.seek 0ml @14:59:07.85 delay 3 seconds @14:59:10.87 ATV.dial :air1 @14:59:11.26 delay 3 seconds @14:59:13.62
CSV.dial CSR @14:59:14.16 Pulling 4ml of CSR.flush @14:59:14.17 CSR.select :flush @14:59:14.34 AS.seek 0.434ml @14:59:14.41
CS.seek 4ml @14:59:19.67 delay 3 seconds @14:59:22.70 ATV.dial :ethanol @14:59:23.30 AS.seek 0.419ml @14:59:25.65
delay 5 seconds @14:59:26.59 delay 3 seconds @14:59:29.61 ATV.dial ASV @14:59:30.67
CSR.select :air @14:59:30.86 CS.seek 10ml @14:59:30.94 AS.seek 0.092ml @14:59:47.22 CSV.dial CTV @14:59:47.25
CTV.dial CSV @14:59:47.75 CBV.dial :waste1 @14:59:47.77 CS.seek 0ml @15:00:14.50 Garaging FlushPuck in Collection Clamp @15:00:14.57 Elbow.reconfigure TightElbow @15:00:14.62 Elbow.seek CC @15:00:14.88 Elbow.reconfigure LooseElbow @15:00:14.99 Forearm.seek CC @15:00:15.97 Hand.close @15:00:16.54 CC.seek :open @15:00:20.91 Forearm.seek :retract @15:00:21.89 Elbow.reconfigure TightElbow @15:00:21.94 Elbow.seek :garage @15:00:25.04 Elbow.reconfigure LooseElbow @15:00:25.14 Forearm.reconfigure TightForearm @15:00:25.19 Forearm.seek :garage @15:00:26.35 Hand.open @15:00:26.99 Forearm.reconfigure LooseForearm @15:00:27.03 Forearm.seek :retract @15:00:28.04 Whole Cell Archive sampling 1500ml @15:00:28.06 Loading wcr puck from tube 3 into Collection Clamp @15:00:28.32 SC.reconfigure SCtight @15:00:28.37 SC.seek 3 @15:00:30.56 SC.reconfigure SCloose @15:00:30.79 Elbow.reconfigure TightElbow @15:00:30.84 Elbow.seek 3 @15:00:32.95 Forearm.reconfigure TightForearm @15:00:33.01 Forearm.seek 3 + 700 counts @15:00:34.07 SE.reconfigure SEpick @15:00:34.15 SE.seek beamBreak @15:00:36.73 SE.configure SEconfig @15:00:36.90 Elbow.seek 3 @15:00:37.01 delay 3 seconds @15:00:37.18 Forearm.seek 3 + 300 counts @15:00:37.47 Forearm.reconfigure LooseForearm @15:00:37.52 Hand.close @15:00:38.19 SE.seek :down @15:00:38.44 Forearm.seek :retract @15:00:39.43 Elbow.seek CC @15:00:40.03 ATV.dial :waste2 @15:00:40.10 SE.coast @15:00:40.76 AS.reconfigure ASconfig @15:00:40.81 AS.seek :empty @15:00:41.13 Elbow.reconfigure LooseElbow @15:00:41.24 Forearm.reconfigure TightForearm @15:00:41.29 Forearm.seek CC @15:00:42.59 CC.seek :closed @15:00:46.46 ATV.dial :air2 @15:00:46.87 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.09 @15:00:46.89 Hand.open @15:00:46.96 AS.reconfigure ASslow1 @15:00:47.01 AS.seek 1ml @15:00:47.61 Forearm.reconfigure LooseForearm @15:00:47.66 Forearm.seek :retract @15:00:48.67 SC.coast @15:00:48.72 collectSample 1500ml w/bubble point of 55psi @15:00:48.73 Priming 7ml @15:00:48.74 Intake.select :close @15:00:48.75 Exhaust.select :close @15:00:48.77 SS.hold @15:00:48.99 Calibrating pressure sensors @15:00:49.01 CTV.dial :air @15:00:50.39 SSV.dial :spit,avoiding: :exhaust @15:00:50.41 SS.reconfigure SSfast @15:00:50.46 SS.seek 5ml @15:00:53.29 SSV.dial CBV @15:00:53.32 CBV.dial SSV @15:00:54.79 delay 3.8 seconds @15:00:58.73 SSV.dial :spit @15:01:00.12 SS.seek 0ml @15:01:02.67 SSV.dial CBV @15:01:04.10 CTV.dial :intake @15:01:04.89 SSV.dial CBV @15:01:04.92 CBV.dial SSV @15:01:04.93 Allowing 40s for 7.0ml of remaining 7.0ml @15:01:04.94 Intake.select :open @15:01:07.12 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:01:07.14 SS.reconfigure SSinhale.with maxSpeed: 150 @15:01:07.19 SS.seek 7ml @15:01:10.67 Vacuum too high -- waiting up to 40s @15:01:13.09 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:01:13.11 SS.reconfigure SSinhale.with maxSpeed: 135 @15:01:13.16 SS.seek 7ml @15:01:15.97 Vacuum too high -- waiting up to 40s @15:01:17.90 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:01:17.92 SS.reconfigure SSinhale.with maxSpeed: 122 @15:01:17.97 SS.seek 7ml @15:01:18.12 Stroke completed -- waiting for pressure to equalize ... @15:01:22.00 Intake.select :close @15:01:22.16 SSV.dial :exhaust @15:01:22.98 Exhaust.select :open @15:01:23.09 SS.reconfigure SSexhale @15:01:23.15 SS.seek 0ml @15:01:26.73 Exhaust.select :close @15:01:26.85 Sampling 1500.0ml @15:01:26.86 Intake.select :close @15:01:26.88 Exhaust.select :close @15:01:26.89 SS.reconfigure SSconfig @15:01:26.94 SS.hold @15:01:27.16 CTV.dial :intake @15:01:27.18 SSV.dial CBV @15:01:27.20 CBV.dial SSV @15:01:28.03 Allowing 130s for 25.0ml of remaining 1500.0ml @15:01:28.04 Intake.select :open @15:01:30.22 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:01:30.24 SS.reconfigure SSinhale.with maxSpeed: 122 @15:01:30.29 SS.seek 25ml @15:01:34.39 Vacuum too high -- waiting up to 40s @15:01:36.23 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:01:36.25 SS.reconfigure SSinhale.with maxSpeed: 110 @15:01:36.30 SS.seek 25ml @15:01:38.78 Vacuum too high -- waiting up to 40s @15:01:40.38 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:01:40.40 SS.reconfigure SSinhale.with maxSpeed: 99 @15:01:40.45 SS.seek 25ml @15:01:42.96 Vacuum too high -- waiting up to 40s @15:01:44.90 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:01:44.92 SS.reconfigure SSinhale.with maxSpeed: 89 @15:01:44.97 SS.seek 25ml @15:01:48.11 Vacuum too high -- waiting up to 40s @15:01:49.48 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:01:49.50 SS.reconfigure SSinhale.with maxSpeed: 80 @15:01:49.55 SS.seek 25ml @15:01:52.24 Vacuum too high -- waiting up to 40s @15:01:53.63 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:01:53.65 SS.reconfigure SSinhale.with maxSpeed: 72 @15:01:53.69 SS.seek 25ml @15:01:57.44 Vacuum too high -- waiting up to 40s @15:01:58.82 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:01:58.84 SS.reconfigure SSinhale.with maxSpeed: 65 @15:01:58.89 SS.seek 25ml @15:02:02.78 Vacuum too high -- waiting up to 40s @15:02:04.16 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:02:04.18 SS.reconfigure SSinhale.with maxSpeed: 59 @15:02:04.23 SS.seek 25ml @15:02:09.05 Vacuum too high -- waiting up to 40s @15:02:10.43 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:02:10.45 SS.reconfigure SSinhale.with maxSpeed: 53 @15:02:10.50 SS.seek 25ml @15:02:13.03 Stroke completed -- waiting for pressure to equalize ... @15:02:16.28 Intake.select :close @15:02:16.45 SSV.dial :exhaust @15:02:17.26 Exhaust.select :open @15:02:17.37 SS.reconfigure SSexhale @15:02:17.43 SS.seek 0ml @15:02:26.40 Exhaust.select :close @15:02:26.52 SSV.dial CBV @15:02:26.55 CBV.dial SSV @15:02:27.38 Allowing 130s for 25.0ml of remaining 1475.0ml @15:02:27.39 Intake.select :open @15:02:29.57 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:02:29.59 SS.reconfigure SSinhale.with maxSpeed: 53 @15:02:29.64 SS.seek 25ml @15:02:38.89 Vacuum too high -- waiting up to 40s @15:02:40.31 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:02:40.33 SS.reconfigure SSinhale.with maxSpeed: 48 @15:02:40.38 SS.seek 25ml @15:02:55.77 Vacuum too high -- waiting up to 40s @15:02:56.87 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:02:56.89 SS.reconfigure SSinhale.with maxSpeed: 43 @15:02:56.94 SS.seek 25ml @15:03:11.06 Stroke completed -- waiting for pressure to equalize ... @15:03:14.31 Intake.select :close @15:03:14.47 SSV.dial :exhaust @15:03:15.29 Exhaust.select :open @15:03:15.40 SS.reconfigure SSexhale @15:03:15.45 SS.seek 0ml @15:03:24.50 Exhaust.select :close @15:03:24.62 SSV.dial CBV @15:03:24.65 CBV.dial SSV @15:03:25.48 Allowing 130s for 25.0ml of remaining 1450.0ml @15:03:25.49 Intake.select :open @15:03:27.68 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:03:27.70 SS.reconfigure SSinhale.with maxSpeed: 43 @15:03:27.75 SS.seek 25ml @15:04:08.60 delay 5 seconds @15:04:09.03 Stroke completed -- waiting for pressure to equalize ... @15:04:12.28 Intake.select :close @15:04:12.44 SSV.dial :exhaust @15:04:13.26 Exhaust.select :open @15:04:13.37 SS.reconfigure SSexhale @15:04:13.43 SS.seek 0ml @15:04:13.61 ATV.dial ASV @15:04:14.53 ASV.dial :SPEdelivery @15:04:14.56 AS.seek 0.02ml @15:04:22.54 Exhaust.select :close @15:04:22.66 SSV.dial CBV @15:04:22.69 CBV.dial SSV @15:04:23.52 Allowing 130s for 25.0ml of remaining 1425.0ml @15:04:23.53 Intake.select :open @15:04:25.72 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:04:25.74 SS.reconfigure SSinhale.with maxSpeed: 43 @15:04:25.79 SS.seek 25ml @15:05:07.25 Stroke completed -- waiting for pressure to equalize ... @15:05:10.48 Intake.select :close @15:05:10.65 SSV.dial :exhaust @15:05:11.46 Exhaust.select :open @15:05:11.57 SS.reconfigure SSexhale @15:05:11.62 SS.seek 0ml @15:05:20.62 Exhaust.select :close @15:05:20.74 SSV.dial CBV @15:05:20.77 CBV.dial SSV @15:05:21.60 Allowing 130s for 25.0ml of remaining 1400.0ml @15:05:21.61 Intake.select :open @15:05:23.80 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:05:23.82 SS.reconfigure SSinhale.with maxSpeed: 43 @15:05:23.87 SS.seek 25ml @15:06:05.61 Stroke completed -- waiting for pressure to equalize ... @15:06:09.93 Intake.select :close @15:06:10.10 SSV.dial :exhaust @15:06:10.94 Exhaust.select :open @15:06:11.06 SS.reconfigure SSexhale @15:06:11.11 SS.seek 0ml @15:06:20.15 Exhaust.select :close @15:06:20.27 SSV.dial CBV @15:06:20.30 CBV.dial SSV @15:06:21.13 Allowing 130s for 25.0ml of remaining 1375.0ml @15:06:21.14 Intake.select :open @15:06:23.33 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:06:23.35 SS.reconfigure SSinhale.with maxSpeed: 43 @15:06:23.40 SS.seek 25ml @15:06:57.05 Vacuum too high -- waiting up to 40s @15:06:58.17 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:06:58.19 SS.reconfigure SSinhale.with maxSpeed: 39 @15:06:58.24 SS.seek 25ml @15:07:07.20 Stroke completed -- waiting for pressure to equalize ... @15:07:10.45 Intake.select :close @15:07:10.61 SSV.dial :exhaust @15:07:11.41 Exhaust.select :open @15:07:11.52 SS.reconfigure SSexhale @15:07:11.57 SS.seek 0ml @15:07:21.00 Exhaust.select :close @15:07:21.13 SSV.dial CBV @15:07:21.15 CBV.dial SSV @15:07:21.99 Allowing 130s for 25.0ml of remaining 1350.0ml @15:07:22.00 Intake.select :open @15:07:24.18 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:07:24.20 SS.reconfigure SSinhale.with maxSpeed: 39 @15:07:24.25 SS.seek 25ml @15:07:31.93 delay 5 seconds @15:07:36.95 ATV.dial :air2 @15:07:37.96 AS.seek 0.52ml @15:08:09.51 Stroke completed -- waiting for pressure to equalize ... @15:08:12.75 Intake.select :close @15:08:12.92 SSV.dial :exhaust @15:08:13.73 Exhaust.select :open @15:08:13.84 SS.reconfigure SSexhale @15:08:13.89 SS.seek 0ml @15:08:23.09 Exhaust.select :close @15:08:23.21 SSV.dial CBV @15:08:23.24 CBV.dial SSV @15:08:24.07 Allowing 130s for 25.0ml of remaining 1325.0ml @15:08:24.08 Intake.select :open @15:08:26.27 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:08:26.29 SS.reconfigure SSinhale.with maxSpeed: 39 @15:08:26.34 SS.seek 25ml @15:09:11.73 Stroke completed -- waiting for pressure to equalize ... @15:09:15.61 Intake.select :close @15:09:15.77 SSV.dial :exhaust @15:09:17.13 Exhaust.select :open @15:09:17.24 SS.reconfigure SSexhale @15:09:17.30 SS.seek 0ml @15:09:18.81 delay 5 seconds @15:09:23.83 ATV.dial ASV @15:09:24.86 ASV.dial :wasteR @15:09:25.22 AS.seek 0.3ml @15:09:26.47 Exhaust.select :close @15:09:26.59 SSV.dial CBV @15:09:26.62 CBV.dial SSV @15:09:27.46 Allowing 130s for 25.0ml of remaining 1300.0ml @15:09:27.47 Intake.select :open @15:09:29.66 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:09:29.68 SS.reconfigure SSinhale.with maxSpeed: 39 @15:09:29.73 SS.seek 25ml @15:10:09.75 delay 20 seconds @15:10:15.34 Stroke completed -- waiting for pressure to equalize ... @15:10:19.21 Intake.select :close @15:10:19.37 SSV.dial :exhaust @15:10:20.19 Exhaust.select :open @15:10:20.30 SS.reconfigure SSexhale @15:10:20.37 SS.seek 0ml @15:10:29.37 Exhaust.select :close @15:10:29.49 SSV.dial CBV @15:10:29.52 CBV.dial SSV @15:10:29.78 AS.seek 0.07ml @15:10:30.34 Allowing 130s for 25.0ml of remaining 1275.0ml @15:10:30.35 Intake.select :open @15:10:32.54 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:10:32.56 SS.reconfigure SSinhale.with maxSpeed: 39 @15:10:32.61 SS.seek 25ml @15:11:16.37 delay 20 seconds @15:11:17.97 Stroke completed -- waiting for pressure to equalize ... @15:11:21.87 Intake.select :close @15:11:22.03 SSV.dial :exhaust @15:11:22.85 Exhaust.select :open @15:11:22.96 SS.reconfigure SSexhale @15:11:23.01 SS.seek 0ml @15:11:32.00 Exhaust.select :close @15:11:32.12 SSV.dial CBV @15:11:32.15 CBV.dial SSV @15:11:32.99 Allowing 130s for 25.0ml of remaining 1250.0ml @15:11:33.00 Intake.select :open @15:11:35.19 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:11:35.21 SS.reconfigure SSinhale.with maxSpeed: 39 @15:11:35.27 SS.seek 25ml @15:11:36.38 ATV.dial :waste2 @15:11:37.12 AS.reconfigure ASconfig @15:11:37.17 AS.seek :empty @15:11:41.56 Elution @15:11:41.57 ATV.dial :air1 @15:11:42.66 AS.reconfigure ASslow1 @15:11:42.71 AS.seek 0.049ml @15:11:53.14 delay 3 seconds @15:11:56.16 ATV.dial :ElutionWater @15:11:56.93 AS.seek 0.109ml @15:12:09.28 delay 3 seconds @15:12:12.30 ATV.dial ASV,via: :air2 @15:12:14.25 ASV.dial :SPEelution @15:12:14.84 AFV.select :carrierSolution @15:12:15.00 AS.reconfigure ASnorm @15:12:15.05 AS.seek 0.759ml @15:12:20.62 Stroke completed -- waiting for pressure to equalize ... @15:12:25.00 Intake.select :close @15:12:25.17 SSV.dial :exhaust @15:12:25.98 Exhaust.select :open @15:12:26.09 SS.reconfigure SSexhale @15:12:26.14 SS.seek 0ml @15:12:35.01 Exhaust.select :close @15:12:35.13 SSV.dial CBV @15:12:35.16 CBV.dial SSV @15:12:35.98 Allowing 130s for 25.0ml of remaining 1225.0ml @15:12:35.99 Intake.select :open @15:12:38.17 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:12:38.19 SS.reconfigure SSinhale.with maxSpeed: 39 @15:12:38.24 SS.seek 25ml @15:12:48.09 delay 3 seconds @15:12:51.11 AFV.select :holdingCoil @15:12:51.28 AS.reconfigure ASslow1 @15:12:51.33 AS.seek 0.474ml @15:13:16.67 Vacuum too high -- waiting up to 40s @15:13:18.02 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:13:18.04 SS.reconfigure SSinhale.with maxSpeed: 35 @15:13:18.09 SS.seek 25ml @15:13:26.42 Stroke completed -- waiting for pressure to equalize ... @15:13:30.27 Intake.select :close @15:13:30.43 SSV.dial :exhaust @15:13:31.26 Exhaust.select :open @15:13:31.37 SS.reconfigure SSexhale @15:13:31.42 SS.seek 0ml @15:13:40.86 Exhaust.select :close @15:13:40.98 SSV.dial CBV @15:13:41.01 CBV.dial SSV @15:13:41.85 Allowing 130s for 25.0ml of remaining 1200.0ml @15:13:41.86 Intake.select :open @15:13:44.05 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:13:44.07 SS.reconfigure SSinhale.with maxSpeed: 35 @15:13:44.12 SS.seek 25ml @15:13:48.94 delay 5 seconds @15:13:54.03 AS.reconfigure ASslow2 @15:13:54.08 AS.seek 0.439ml @15:14:29.42 delay 1:00 @15:14:34.66 Stroke completed -- waiting for pressure to equalize ... @15:14:38.54 Intake.select :close @15:14:38.71 SSV.dial :exhaust @15:14:39.52 Exhaust.select :open @15:14:39.63 SS.reconfigure SSexhale @15:14:39.69 SS.seek 0ml @15:14:48.57 Exhaust.select :close @15:14:48.69 SSV.dial CBV @15:14:48.72 CBV.dial SSV @15:14:49.54 Allowing 130s for 25.0ml of remaining 1175.0ml @15:14:49.55 Intake.select :open @15:14:51.74 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:14:51.76 SS.reconfigure SSinhale.with maxSpeed: 35 @15:14:51.81 SS.seek 25ml @15:15:29.45 pushing eluate to holding coil @15:15:29.52 AS.seek 0.279ml @15:15:42.62 Stroke completed -- waiting for pressure to equalize ... @15:15:47.02 Intake.select :close @15:15:47.18 SSV.dial :exhaust @15:15:48.01 Exhaust.select :open @15:15:48.12 SS.reconfigure SSexhale @15:15:48.18 SS.seek 0ml @15:15:57.41 Exhaust.select :close @15:15:57.52 SSV.dial CBV @15:15:57.55 CBV.dial SSV @15:15:58.38 Allowing 130s for 25.0ml of remaining 1150.0ml @15:15:58.39 Intake.select :open @15:16:00.58 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:16:00.60 SS.reconfigure SSinhale.with maxSpeed: 35 @15:16:00.65 SS.seek 25ml @15:16:51.09 Stroke completed -- waiting for pressure to equalize ... @15:16:55.60 Intake.select :close @15:16:55.76 SSV.dial :exhaust @15:16:56.60 Exhaust.select :open @15:16:56.71 SS.reconfigure SSexhale @15:16:56.76 SS.seek 0ml @15:17:06.12 Exhaust.select :close @15:17:06.24 SSV.dial CBV @15:17:06.27 CBV.dial SSV @15:17:07.09 Allowing 130s for 25.0ml of remaining 1125.0ml @15:17:07.10 Intake.select :open @15:17:09.28 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:17:09.30 SS.reconfigure SSinhale.with maxSpeed: 35 @15:17:09.35 SS.seek 25ml @15:17:59.76 Stroke completed -- waiting for pressure to equalize ... @15:18:04.89 Intake.select :close @15:18:05.05 SSV.dial :exhaust @15:18:05.87 Exhaust.select :open @15:18:05.99 SS.reconfigure SSexhale @15:18:06.04 SS.seek 0ml @15:18:10.72 delay 30 seconds @15:18:14.81 Exhaust.select :close @15:18:14.93 SSV.dial CBV @15:18:14.96 CBV.dial SSV @15:18:15.80 Allowing 130s for 25.0ml of remaining 1100.0ml @15:18:15.81 Intake.select :open @15:18:18.00 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:18:18.02 SS.reconfigure SSinhale.with maxSpeed: 35 @15:18:18.06 SS.seek 25ml @15:18:40.76 ATV.dial :waste,via: :air3 @15:18:42.37 AS.reconfigure ASconfig @15:18:42.42 AS.seek :empty @15:18:58.84 Parking Bleach in LysateSource line and SPEcolumn @15:18:58.85 ATV.dial :air3 @15:18:59.65 AS.reconfigure ASslow1 @15:18:59.70 AS.seek 0.029ml @15:19:00.46 Vacuum too high -- waiting up to 40s @15:19:02.11 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:19:02.13 SS.reconfigure SSinhale.with maxSpeed: 32 @15:19:02.18 SS.seek 25ml @15:19:06.10 ATV.dial :ElutionWater @15:19:06.71 AS.seek 0.059ml @15:19:11.44 Stroke completed -- waiting for pressure to equalize ... @15:19:12.99 ATV.dial :air3 @15:19:13.60 AS.seek 0.069ml @15:19:15.96 delay 2 seconds @15:19:16.00 Intake.select :close @15:19:16.17 SSV.dial :exhaust @15:19:16.99 Exhaust.select :open @15:19:17.10 SS.reconfigure SSexhale @15:19:17.15 SS.seek 0ml @15:19:17.98 ATV.dial :bleach @15:19:18.44 AS.seek 0.769ml @15:19:26.50 Exhaust.select :close @15:19:26.62 SSV.dial CBV @15:19:26.65 CBV.dial SSV @15:19:27.49 Allowing 130s for 25.0ml of remaining 1075.0ml @15:19:27.50 Intake.select :open @15:19:29.69 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:19:29.71 SS.reconfigure SSinhale.with maxSpeed: 32 @15:19:29.76 SS.seek 25ml @15:20:25.02 Stroke completed -- waiting for pressure to equalize ... @15:20:31.00 Intake.select :close @15:20:31.70 SSV.dial :exhaust @15:20:32.59 Exhaust.select :open @15:20:32.72 SS.reconfigure SSexhale @15:20:32.77 SS.seek 0ml @15:20:41.63 Exhaust.select :close @15:20:41.74 SSV.dial CBV @15:20:41.77 CBV.dial SSV @15:20:42.60 Allowing 130s for 25.0ml of remaining 1050.0ml @15:20:42.61 Intake.select :open @15:20:44.79 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:20:44.81 SS.reconfigure SSinhale.with maxSpeed: 32 @15:20:44.87 SS.seek 25ml @15:21:39.55 delay 3 seconds @15:21:40.14 Stroke completed -- waiting for pressure to equalize ... @15:21:42.67 ATV.dial :air3 @15:21:43.20 delay 3 seconds @15:21:45.33 Intake.select :close @15:21:45.50 SSV.dial :exhaust @15:21:46.29 AS.seek 0.794ml @15:21:46.35 Exhaust.select :open @15:21:46.47 SS.reconfigure SSexhale @15:21:46.53 SS.seek 0ml @15:21:51.64 delay 3 seconds @15:21:54.66 ATV.dial :bleach @15:21:55.26 AS.seek 0.779ml @15:21:55.82 Exhaust.select :close @15:21:55.93 SSV.dial CBV @15:21:55.96 CBV.dial SSV @15:21:57.35 Allowing 130s for 25.0ml of remaining 1025.0ml @15:21:57.36 Intake.select :open @15:21:58.53 delay 3 seconds @15:21:59.54 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:21:59.56 SS.reconfigure SSinhale.with maxSpeed: 32 @15:21:59.61 SS.seek 25ml @15:22:01.55 ATV.dial :AIV @15:22:02.85 AS.reconfigure ASconfig @15:22:02.91 AS.seek 0.015ml @15:22:47.13 ATV.dial :waste @15:22:48.55 AS.seek :empty @15:22:49.77 ATV.dial :air3 @15:22:50.43 AS.reconfigure ASslow1 @15:22:50.48 AS.seek 0.009ml @15:22:52.84 delay 2 seconds @15:22:54.70 Stroke completed -- waiting for pressure to equalize ... @15:22:54.85 ATV.dial :bleach @15:22:55.39 AS.seek 0.579ml @15:23:00.00 Intake.select :close @15:23:00.16 SSV.dial :exhaust @15:23:00.99 Exhaust.select :open @15:23:01.10 SS.reconfigure SSexhale @15:23:01.16 SS.seek 0ml @15:23:10.72 Exhaust.select :close @15:23:10.84 SSV.dial CBV @15:23:10.87 CBV.dial SSV @15:23:11.71 Allowing 130s for 25.0ml of remaining 1000.0ml @15:23:11.72 Intake.select :open @15:23:13.91 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:23:13.93 SS.reconfigure SSinhale.with maxSpeed: 32 @15:23:13.98 SS.seek 25ml @15:23:53.81 Vacuum too high -- waiting up to 40s @15:23:55.19 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:23:55.21 SS.reconfigure SSinhale.with maxSpeed: 29 @15:23:55.26 SS.seek 25ml @15:24:12.40 Stroke completed -- waiting for pressure to equalize ... @15:24:17.57 Intake.select :close @15:24:17.74 SSV.dial :exhaust @15:24:18.57 Exhaust.select :open @15:24:18.68 SS.reconfigure SSexhale @15:24:18.73 SS.seek 0ml @15:24:27.87 Exhaust.select :close @15:24:27.99 SSV.dial CBV @15:24:28.02 CBV.dial SSV @15:24:28.85 Allowing 130s for 25.0ml of remaining 975.0ml @15:24:28.86 Intake.select :open @15:24:31.05 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:24:31.07 SS.reconfigure SSinhale.with maxSpeed: 29 @15:24:31.11 SS.seek 25ml @15:24:50.33 delay 3 seconds @15:24:53.35 ATV.dial :air3 @15:24:53.88 delay 3 seconds @15:24:56.97 AS.seek 0.604ml @15:25:02.25 delay 3 seconds @15:25:05.27 ATV.dial :bleach @15:25:06.43 AS.seek 0.589ml @15:25:09.75 delay 3 seconds @15:25:12.77 ATV.dial :ASV @15:25:13.75 ASV.dial :wasteR @15:25:14.32 AS.seek 0ml @15:25:31.50 Stroke completed -- waiting for pressure to equalize ... @15:25:36.63 Intake.select :close @15:25:36.79 SSV.dial :exhaust @15:25:37.62 Exhaust.select :open @15:25:37.73 SS.reconfigure SSexhale @15:25:37.79 SS.seek 0ml @15:25:47.14 Exhaust.select :close @15:25:47.26 SSV.dial CBV @15:25:47.29 CBV.dial SSV @15:25:48.13 Allowing 130s for 25.0ml of remaining 950.0ml @15:25:48.14 Intake.select :open @15:25:50.33 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:25:50.35 SS.reconfigure SSinhale.with maxSpeed: 29 @15:25:50.40 SS.seek 25ml @15:26:51.20 Stroke completed -- waiting for pressure to equalize ... @15:26:56.99 Intake.select :close @15:26:57.16 SSV.dial :exhaust @15:26:57.98 Exhaust.select :open @15:26:58.09 SS.reconfigure SSexhale @15:26:58.15 SS.seek 0ml @15:27:07.20 Exhaust.select :close @15:27:07.32 SSV.dial CBV @15:27:07.35 CBV.dial SSV @15:27:08.18 Allowing 130s for 25.0ml of remaining 925.0ml @15:27:08.19 Intake.select :open @15:27:10.38 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:27:10.40 SS.reconfigure SSinhale.with maxSpeed: 29 @15:27:10.45 SS.seek 25ml @15:27:13.02 delay 1:00 @15:28:06.77 Vacuum too high -- waiting up to 40s @15:28:08.41 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:28:08.43 SS.reconfigure SSinhale.with maxSpeed: 26 @15:28:08.48 SS.seek 25ml @15:28:13.04 AFV.select :carrierSolution @15:28:13.15 delay 2 seconds @15:28:13.29 Stroke completed -- waiting for pressure to equalize ... @15:28:15.23 AS.reconfigure ASconfig @15:28:15.28 AS.seek 1ml @15:28:18.39 Intake.select :close @15:28:18.55 SSV.dial :exhaust @15:28:19.37 Exhaust.select :open @15:28:19.49 SS.reconfigure SSexhale @15:28:19.55 SS.seek 0ml @15:28:29.06 Exhaust.select :close @15:28:29.18 SSV.dial CBV @15:28:29.21 CBV.dial SSV @15:28:30.05 Allowing 130s for 25.0ml of remaining 900.0ml @15:28:30.06 Intake.select :open @15:28:32.25 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:28:32.27 SS.reconfigure SSinhale.with maxSpeed: 26 @15:28:32.32 SS.seek 25ml @15:29:13.08 delay 1 second @15:29:14.10 AFV.select :holdingCoil @15:29:14.21 delay 1 second @15:29:15.23 ATV.dial ASV @15:29:15.25 ASV.dial :wasteR @15:29:15.27 AS.reconfigure ASslow1 @15:29:15.31 AS.seek 0ml @15:29:39.75 Stroke completed -- waiting for pressure to equalize ... @15:29:44.89 Intake.select :close @15:29:45.06 SSV.dial :exhaust @15:29:45.87 Exhaust.select :open @15:29:45.99 SS.reconfigure SSexhale @15:29:46.05 SS.seek 0ml @15:29:55.37 Exhaust.select :close @15:29:55.49 SSV.dial CBV @15:29:55.52 CBV.dial SSV @15:29:56.35 Allowing 130s for 25.0ml of remaining 875.0ml @15:29:56.36 Intake.select :open @15:29:58.55 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:29:58.57 SS.reconfigure SSinhale.with maxSpeed: 26 @15:29:58.62 SS.seek 25ml @15:31:05.79 Stroke completed -- waiting for pressure to equalize ... @15:31:11.58 Intake.select :close @15:31:11.75 SSV.dial :exhaust @15:31:12.57 Exhaust.select :open @15:31:12.68 SS.reconfigure SSexhale @15:31:12.73 SS.seek 0ml @15:31:22.09 Exhaust.select :close @15:31:22.21 SSV.dial CBV @15:31:22.24 CBV.dial SSV @15:31:23.09 Allowing 130s for 25.0ml of remaining 850.0ml @15:31:23.10 Intake.select :open @15:31:25.29 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:31:25.31 SS.reconfigure SSinhale.with maxSpeed: 26 @15:31:25.36 SS.seek 25ml @15:32:32.70 Stroke completed -- waiting for pressure to equalize ... @15:32:36.70 delay 20 seconds @15:32:39.04 Intake.select :close @15:32:39.21 SSV.dial :exhaust @15:32:40.02 Exhaust.select :open @15:32:40.14 SS.reconfigure SSexhale @15:32:40.20 SS.seek 0ml @15:32:49.42 Exhaust.select :close @15:32:49.54 SSV.dial CBV @15:32:49.57 CBV.dial SSV @15:32:50.40 Allowing 130s for 25.0ml of remaining 825.0ml @15:32:50.41 Intake.select :open @15:32:52.60 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:32:52.62 SS.reconfigure SSinhale.with maxSpeed: 26 @15:32:52.67 SS.seek 25ml @15:32:56.72 ATV.dial :air3 @15:32:58.15 AS.seek 0.59ml @15:33:57.55 Vacuum too high -- waiting up to 40s @15:33:59.17 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:33:59.19 SS.reconfigure SSinhale.with maxSpeed: 23 @15:33:59.24 SS.seek 25ml @15:34:02.65 Stroke completed -- waiting for pressure to equalize ... @15:34:07.83 Intake.select :close @15:34:07.99 SSV.dial :exhaust @15:34:08.80 Exhaust.select :open @15:34:08.91 SS.reconfigure SSexhale @15:34:08.96 SS.seek 0ml @15:34:18.14 Exhaust.select :close @15:34:18.26 SSV.dial CBV @15:34:18.29 CBV.dial SSV @15:34:19.13 Allowing 130s for 25.0ml of remaining 800.0ml @15:34:19.14 Intake.select :open @15:34:21.32 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:34:21.34 SS.reconfigure SSinhale.with maxSpeed: 23 @15:34:21.39 SS.seek 25ml @15:34:57.08 delay 3 seconds @15:35:00.10 ATV.dial :ASV @15:35:01.43 ASV.dial :wasteR @15:35:01.51 AS.seek 0.09ml @15:35:37.54 Stroke completed -- waiting for pressure to equalize ... @15:35:43.97 Intake.select :close @15:35:44.14 SSV.dial :exhaust @15:35:44.94 Exhaust.select :open @15:35:45.05 SS.reconfigure SSexhale @15:35:45.10 SS.seek 0ml @15:35:54.11 Exhaust.select :close @15:35:54.23 SSV.dial CBV @15:35:54.26 CBV.dial SSV @15:35:55.08 Allowing 130s for 25.0ml of remaining 775.0ml @15:35:55.64 Intake.select :open @15:35:57.83 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:35:57.85 SS.reconfigure SSinhale.with maxSpeed: 23 @15:35:57.90 SS.seek 25ml @15:36:42.36 delay 30 seconds @15:37:12.38 ATV.dial :extSrc @15:37:13.11 delay 3 seconds @15:37:14.31 Stroke completed -- waiting for pressure to equalize ... @15:37:16.21 ATV.dial :ElutionWater @15:37:17.28 AS.seek 0.12ml @15:37:21.42 Intake.select :close @15:37:21.58 SSV.dial :exhaust @15:37:22.40 Exhaust.select :open @15:37:22.51 SS.reconfigure SSexhale @15:37:22.57 SS.seek 0ml @15:37:23.60 ATV.dial :air3 @15:37:24.20 AS.seek 0.13ml @15:37:26.50 delay 2 seconds @15:37:28.51 ATV.dial :bleach @15:37:28.97 AS.seek 0.23ml @15:37:32.36 Exhaust.select :close @15:37:32.48 SSV.dial CBV @15:37:32.51 CBV.dial SSV @15:37:33.35 Allowing 130s for 25.0ml of remaining 750.0ml @15:37:33.36 Intake.select :open @15:37:35.54 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:37:35.56 SS.reconfigure SSinhale.with maxSpeed: 23 @15:37:35.61 SS.seek 25ml @15:37:49.36 delay 3 seconds @15:37:52.38 ATV.dial :air3 @15:37:52.93 delay 3 seconds @15:37:56.02 AS.seek 0.255ml @15:38:01.32 delay 3 seconds @15:38:04.34 ATV.dial :bleach @15:38:04.93 AS.seek 0.24ml @15:38:08.27 delay 3 seconds @15:38:11.29 ATV.dial :ASV @15:38:12.29 ASV.dial :SPEdelivery @15:38:12.64 AFV.select :carrierSolution @15:38:12.76 delay 2 seconds @15:38:14.79 AS.reconfigure ASconfig @15:38:14.84 AS.seek 0.5ml @15:38:30.08 delay 1 second @15:38:31.09 AFV.select :holdingCoil @15:38:31.25 AS.reconfigure ASslow1 @15:38:31.31 AS.seek 0.17ml @15:38:51.84 Stroke completed -- waiting for pressure to equalize ... @15:38:58.89 Intake.select :close @15:38:59.06 SSV.dial :exhaust @15:38:59.87 Exhaust.select :open @15:38:59.98 SS.reconfigure SSexhale @15:39:00.04 SS.seek 0ml @15:39:09.17 Exhaust.select :close @15:39:09.29 SSV.dial CBV @15:39:09.87 CBV.dial SSV @15:39:10.69 Allowing 130s for 25.0ml of remaining 725.0ml @15:39:10.70 Intake.select :open @15:39:12.88 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:39:12.90 SS.reconfigure SSinhale.with maxSpeed: 23 @15:39:12.95 SS.seek 25ml @15:39:37.97 SPE.hold 2:46:40,95C @15:39:38.01 delay 3:00 @15:40:12.80 Vacuum too high -- waiting up to 40s @15:40:14.44 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:40:14.46 SS.reconfigure SSinhale.with maxSpeed: 21 @15:40:14.51 SS.seek 25ml @15:40:32.75 Stroke completed -- waiting for pressure to equalize ... @15:40:39.82 Intake.select :close @15:40:39.98 SSV.dial :exhaust @15:40:40.82 Exhaust.select :open @15:40:40.94 SS.reconfigure SSexhale @15:40:41.00 SS.seek 0ml @15:40:50.36 Exhaust.select :close @15:40:50.48 SSV.dial CBV @15:40:50.51 CBV.dial SSV @15:40:51.38 Allowing 130s for 25.0ml of remaining 700.0ml @15:40:51.39 Intake.select :open @15:40:53.58 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:40:53.60 SS.reconfigure SSinhale.with maxSpeed: 21 @15:40:53.65 SS.seek 25ml @15:42:16.72 Stroke completed -- waiting for pressure to equalize ... @15:42:24.40 Intake.select :close @15:42:24.56 SSV.dial :exhaust @15:42:25.40 Exhaust.select :open @15:42:25.51 SS.reconfigure SSexhale @15:42:25.57 SS.seek 0ml @15:42:34.79 Exhaust.select :close @15:42:34.91 SSV.dial CBV @15:42:34.94 CBV.dial SSV @15:42:35.79 Allowing 130s for 25.0ml of remaining 675.0ml @15:42:35.80 Intake.select :open @15:42:37.99 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:42:38.01 SS.reconfigure SSinhale.with maxSpeed: 21 @15:42:38.05 SS.seek 25ml @15:42:38.11 AFV.select :carrierSolution @15:42:38.22 delay 2 seconds @15:42:40.25 AS.reconfigure ASconfig @15:42:40.30 AS.seek 1ml @15:43:28.39 delay 1 second @15:43:29.40 AFV.select :holdingCoil @15:43:29.51 delay 1 second @15:43:30.53 ASV.dial :wasteD @15:43:31.55 AS.seek 0ml @15:44:01.20 Stroke completed -- waiting for pressure to equalize ... @15:44:10.14 Intake.select :close @15:44:10.30 SSV.dial :exhaust @15:44:11.13 Exhaust.select :open @15:44:11.24 SS.reconfigure SSexhale @15:44:11.31 SS.seek 0ml @15:44:20.68 Exhaust.select :close @15:44:20.80 SSV.dial CBV @15:44:20.85 CBV.dial SSV @15:44:21.68 Allowing 130s for 25.0ml of remaining 650.0ml @15:44:21.69 Intake.select :open @15:44:23.87 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:44:23.89 SS.reconfigure SSinhale.with maxSpeed: 21 @15:44:23.94 SS.seek 25ml @15:44:29.33 Priming Eluate @15:44:29.34 AFV.select :carrierSolution @15:44:29.45 delay 2 seconds @15:44:31.48 AS.seek 0.5ml @15:45:00.54 delay 1 second @15:45:01.56 AFV.select :holdingCoil @15:45:01.67 ATV.dial ASV @15:45:01.68 ASV.dial :SPErecovery @15:45:02.26 Swish #1 of 2 @15:45:02.34 AS.reconfigure ASslow0 @15:45:02.39 AS.seek 0.6ml @15:45:12.84 AS.seek 0.5ml @15:45:23.23 Swish #2 of 2 @15:45:23.32 AS.seek 0.6ml @15:45:27.02 Vacuum too high -- waiting up to 40s @15:45:28.98 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:45:29.00 SS.reconfigure SSinhale.with maxSpeed: 19 @15:45:29.05 SS.seek 25ml @15:45:33.82 AS.seek 0.5ml @15:45:44.24 delay 3 seconds @15:45:47.33 AS.reconfigure ASslow1 @15:45:47.38 AS.seek 0.684ml @15:45:51.65 Stroke completed -- waiting for pressure to equalize ... @15:45:59.90 Intake.select :close @15:46:00.07 SSV.dial :exhaust @15:46:00.91 Exhaust.select :open @15:46:01.03 SS.reconfigure SSexhale @15:46:01.09 SS.seek 0ml @15:46:10.39 Exhaust.select :close @15:46:10.51 SSV.dial CBV @15:46:10.54 CBV.dial SSV @15:46:11.38 Allowing 130s for 25.0ml of remaining 625.0ml @15:46:11.39 Intake.select :open @15:46:13.58 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:46:13.60 SS.reconfigure SSinhale.with maxSpeed: 19 @15:46:13.65 SS.seek 25ml @15:46:24.66 delay 3 seconds @15:46:27.69 Eluate primed @15:46:27.71 ATV.dial :waste2 @15:46:28.29 AS.reconfigure ASnorm @15:46:28.35 AS.seek 0ml @15:47:03.15 SPE.coast @15:47:03.24 ASV.dial :SPErecovery @15:47:03.26 Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1115h150ml.pcr @15:47:03.28 Building PCR Slug from ASV with MM1 mix, PP2 primer @15:47:03.30 Priming with carrier @15:47:03.31 ATV.dial ARV @15:47:04.23 ARV.dial :carrier @15:47:04.60 AS.seek 0.5ml @15:47:30.15 AFV.select :carrierSolution @15:47:30.27 delay 2 seconds @15:47:32.30 AS.reconfigure ASconfig @15:47:32.35 AS.seek 1ml @15:47:45.53 Stroke completed -- waiting for pressure to equalize ... @15:47:54.55 Intake.select :close @15:47:54.72 SSV.dial :exhaust @15:47:55.54 Exhaust.select :open @15:47:55.65 SS.reconfigure SSexhale @15:47:55.72 SS.seek 0ml @15:48:01.41 delay 1 second @15:48:02.43 AFV.select :holdingCoil @15:48:02.54 delay 1 second @15:48:03.56 ATV.dial :waste @15:48:04.96 AS.reconfigure ASnorm @15:48:05.01 AS.seek :empty @15:48:05.09 Exhaust.select :close @15:48:05.20 SSV.dial CBV @15:48:05.23 CBV.dial SSV @15:48:06.10 Allowing 130s for 25.0ml of remaining 600.0ml @15:48:06.11 Intake.select :open @15:48:08.30 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:48:08.32 SS.reconfigure SSinhale.with maxSpeed: 19 @15:48:08.36 SS.seek 25ml @15:48:55.76 AFV.select :carrierSolution @15:48:55.88 delay 2 seconds @15:48:57.91 AS.reconfigure ASconfig @15:48:57.96 AS.seek 0.5ml @15:49:27.04 delay 1 second @15:49:28.05 AFV.select :holdingCoil @15:49:28.16 Building Slug @15:49:28.17 ATV.dial :ARV @15:49:29.80 delay 2 seconds @15:49:31.82 ARV.dial :air1 @15:49:32.22 delay 2 seconds @15:49:34.31 AS.reconfigure ASslow2 @15:49:34.36 AS.seek 0.506ml @15:49:40.48 Stroke completed -- waiting for pressure to equalize ... @15:49:40.55 delay 2 seconds @15:49:42.96 ARV.dial :MM1 @15:49:43.56 delay 2 seconds @15:49:45.58 Adding MM1 @15:49:45.66 AS.seek 0.524ml @15:49:50.69 Intake.select :close @15:49:50.86 SSV.dial :exhaust @15:49:51.69 Exhaust.select :open @15:49:51.80 SS.reconfigure SSexhale @15:49:51.86 SS.seek 0ml @15:50:01.26 Exhaust.select :close @15:50:01.38 SSV.dial CBV @15:50:01.41 CBV.dial SSV @15:50:02.26 Allowing 130s for 25.0ml of remaining 575.0ml @15:50:02.27 Intake.select :open @15:50:03.98 delay 25 seconds @15:50:04.46 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:50:04.48 SS.reconfigure SSinhale.with maxSpeed: 19 @15:50:04.53 SS.seek 25ml @15:50:29.00 ARV.dial :PP2 @15:50:29.40 Adding PP2 @15:50:29.42 delay 2 seconds @15:50:31.50 AS.seek 0.53ml @15:50:37.73 delay 10 seconds @15:50:47.75 ARV.dial :air2 @15:50:48.16 adding ARV air @15:50:48.18 delay 2 seconds @15:50:50.26 AS.seek 0.536ml @15:50:56.49 delay 5 seconds @15:51:01.51 ARV.dial :carrier @15:51:02.73 delay 7 seconds @15:51:03.71 Vacuum too high -- waiting up to 40s @15:51:05.61 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:51:05.63 SS.reconfigure SSinhale.with maxSpeed: 17 @15:51:05.68 SS.seek 25ml @15:51:09.82 AS.seek 0.594ml @15:51:42.89 Stroke completed -- waiting for pressure to equalize ... @15:51:52.43 Intake.select :close @15:51:52.59 SSV.dial :exhaust @15:51:53.40 Exhaust.select :open @15:51:53.52 SS.reconfigure SSexhale @15:51:53.58 SS.seek 0ml @15:52:02.86 Exhaust.select :close @15:52:02.98 SSV.dial CBV @15:52:03.01 CBV.dial SSV @15:52:03.83 Allowing 130s for 25.0ml of remaining 550.0ml @15:52:03.84 Intake.select :open @15:52:06.02 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:52:06.04 SS.reconfigure SSinhale.with maxSpeed: 17 @15:52:06.09 SS.seek 25ml @15:52:08.26 delay 5 seconds @15:52:13.28 ATV.dial :ASV @15:52:13.68 delay 2 seconds @15:52:15.78 AS.seek 0.6ml @15:52:22.10 delay 4 seconds @15:52:26.12 ATV.dial ARV @15:52:26.65 delay 2 seconds @15:52:28.67 ARV.dial :carrier @15:52:28.69 delay 2 seconds @15:52:30.79 AS.seek 0.65ml @15:53:21.27 delay 4 seconds @15:53:25.29 ATV.dial PCR @15:53:25.88 ASV.dial :waste1 @15:53:26.64 ARV.dial :air1 @15:53:27.02 Positioning Slug by 0.324ml @15:53:27.11 AS.seek 0.326ml @15:53:49.12 Stroke completed -- waiting for pressure to equalize ... @15:53:59.31 Intake.select :close @15:53:59.48 SSV.dial :exhaust @15:54:00.30 Exhaust.select :open @15:54:00.41 SS.reconfigure SSexhale @15:54:00.47 SS.seek 0ml @15:54:09.75 Exhaust.select :close @15:54:09.87 SSV.dial CBV @15:54:09.90 CBV.dial SSV @15:54:10.73 Allowing 130s for 25.0ml of remaining 525.0ml @15:54:10.74 Intake.select :open @15:54:12.93 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:54:12.95 SS.reconfigure SSinhale.with maxSpeed: 17 @15:54:13.00 SS.seek 25ml @15:55:56.23 Stroke completed -- waiting for pressure to equalize ... @15:56:07.06 Intake.select :close @15:56:07.22 SSV.dial :exhaust @15:56:08.04 Exhaust.select :open @15:56:08.15 SS.reconfigure SSexhale @15:56:08.21 SS.seek 0ml @15:56:17.62 Exhaust.select :close @15:56:17.74 SSV.dial CBV @15:56:17.77 CBV.dial SSV @15:56:18.62 Allowing 130s for 25.0ml of remaining 500.0ml @15:56:18.63 Intake.select :open @15:56:20.82 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:56:20.84 SS.reconfigure SSinhale.with maxSpeed: 17 @15:56:20.89 SS.seek 25ml @15:57:30.42 Vacuum too high -- waiting up to 40s @15:57:32.57 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:57:32.59 SS.reconfigure SSinhale.with maxSpeed: 15 @15:57:32.64 SS.seek 25ml @15:58:10.82 Stroke completed -- waiting for pressure to equalize ... @15:58:21.05 Intake.select :close @15:58:21.21 SSV.dial :exhaust @15:58:22.04 Exhaust.select :open @15:58:22.15 SS.reconfigure SSexhale @15:58:22.21 SS.seek 0ml @15:58:31.51 Exhaust.select :close @15:58:31.63 SSV.dial CBV @15:58:31.66 CBV.dial SSV @15:58:32.48 Allowing 130s for 25.0ml of remaining 475.0ml @15:58:32.49 Intake.select :open @15:58:34.68 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:58:34.70 SS.reconfigure SSinhale.with maxSpeed: 15 @15:58:34.75 SS.seek 25ml @15:58:53.18 delay 5 seconds @15:58:58.20 ATV.dial ARV @15:58:58.95 ASV.dial :PCRlock @15:58:59.32 delay 5 seconds @15:59:04.35 Running Ss Profile60C350 thermocycling @15:59:04.36 PCR.program Profile60C350 @15:59:04.51 Profile60C350 should complete in < 1:48:05 @15:59:04.52 PCR.run 2:20:06.25 @16:00:31.23 Stroke completed -- waiting for pressure to equalize ... @16:00:42.51 SS.reconfigure SSconfig @16:00:42.56 SS.hold @16:00:42.69 Intake.select :close @16:00:42.87 ShallowSampler::Clogged in WCR -- progress too slow after 1050.0ml @16:00:42.88 SSV.dial :exhaust @16:00:43.69 Exhaust.select :open @16:00:43.80 SS.reconfigure SSexhale @16:00:43.86 SS.seek 0ml @16:00:53.25 Exhaust.select :close @16:00:53.37 Sampled 1050.0ml @16:00:53.39 Drying puck before fixing for 4:00 @16:00:53.40 CBV.dial :waste2b @16:00:54.22 delay 1:00 @16:01:54.25 CSV.dial :air @16:01:54.72 CS.reconfigure CSfast @16:01:54.78 CS.seek 10ml @16:02:19.43 Pumping wcr puck from tube 3 up to 55psi... @16:02:19.44 CBV.dial SSV @16:02:20.84 CBV.dial between CSV and waste2b @16:02:21.87 CSV.dial between air and CTV @16:02:22.22 CS.seek 6.847ml @16:02:30.61 CTV.dial CSV,avoiding: :air @16:02:30.64 CSV.dial CTV @16:02:31.52 CS.reconfigure CSpressurize @16:02:31.58 CS.seek 0ml @16:02:49.64 CSV.dial :air @16:02:50.13 CS.reconfigure CSfast @16:02:50.19 CS.seek 10ml @16:03:14.71 CSV.dial between air and CTV @16:03:15.08 CS.seek 2.543ml @16:03:33.83 CTV.dial CSV,avoiding: :air @16:03:33.86 CSV.dial CTV @16:03:34.20 CS.reconfigure CSpressurize @16:03:34.25 CS.seek 0ml @16:03:39.04 CBV.dial :waste2b @16:03:39.40 CS.reconfigure CSconfig @16:03:39.45 CS.seek :empty @16:03:42.65 delay 1:00 @16:04:42.68 CTV.dial :air,avoiding: :intake @16:04:44.09 Fix #1 of 2 (with rnal) for 2:00 @16:04:44.11 CSV.dial CSR @16:04:44.64 Pulling 2ml of CSR.rnal @16:04:44.65 CSR.select :rnal @16:04:44.77 CS.seek 2ml @16:04:50.87 delay 5 seconds @16:04:55.89 CSR.select :air @16:04:56.02 CS.reconfigure CSfast @16:04:56.08 CS.seek 10ml @16:05:15.74 Pumping wcr puck from tube 3 up to 55psi... @16:05:15.76 CBV.dial SSV @16:05:17.11 CBV.dial between CSV and waste2b @16:05:18.16 CSV.dial between air and CTV @16:05:18.81 CS.seek 6.847ml @16:05:27.49 CTV.dial CSV,avoiding: :air @16:05:27.52 CSV.dial CTV @16:05:28.99 CS.reconfigure CSpressurize @16:05:29.05 CS.seek 0ml @16:05:47.15 CSV.dial :air @16:05:47.63 CS.reconfigure CSfast @16:05:47.69 CS.seek 10ml @16:06:12.21 CSV.dial between air and CTV @16:06:12.58 CS.seek 2.054ml @16:06:32.12 CTV.dial CSV,avoiding: :air @16:06:32.15 CSV.dial CTV @16:06:32.49 CS.reconfigure CSpressurize @16:06:32.55 CS.seek 0ml @16:06:33.98 delay 2:00 @16:08:34.01 CBV.dial :waste2b @16:08:34.35 CS.reconfigure CSconfig @16:08:34.40 CS.seek :empty @16:08:39.72 delay 1:00 @16:09:39.75 CTV.dial :air,avoiding: :intake @16:09:41.15 Fix #2 of 2 (with rnal) for 2:00 @16:09:41.17 CSV.dial CSR @16:09:41.71 Pulling 2ml of CSR.rnal @16:09:41.72 CSR.select :rnal @16:09:41.84 CS.seek 2ml @16:09:48.06 delay 5 seconds @16:09:53.08 CSR.select :air @16:09:53.21 CS.reconfigure CSfast @16:09:53.27 CS.seek 10ml @16:10:13.00 Pumping wcr puck from tube 3 up to 55psi... @16:10:13.01 CBV.dial SSV @16:10:14.38 CBV.dial between CSV and waste2b @16:10:15.43 CSV.dial between air and CTV @16:10:16.08 CS.seek 6.823ml @16:10:24.59 CTV.dial CSV,avoiding: :air @16:10:24.62 CSV.dial CTV @16:10:26.07 CS.reconfigure CSpressurize @16:10:26.13 CS.seek 0ml @16:10:44.22 CSV.dial :air @16:10:44.82 CS.reconfigure CSfast @16:10:44.88 CS.seek 10ml @16:11:09.40 CSV.dial between air and CTV @16:11:09.74 CS.seek 2.056ml @16:11:29.47 CTV.dial CSV,avoiding: :air @16:11:29.50 CSV.dial CTV @16:11:29.85 CS.reconfigure CSpressurize @16:11:29.90 CS.seek 0ml @16:11:31.33 delay 2:00 @16:13:31.36 CBV.dial :waste2b @16:13:31.72 CS.reconfigure CSconfig @16:13:31.77 CS.seek :empty @16:13:37.22 delay 1:00 @16:14:37.25 CTV.dial :air,avoiding: :intake @16:14:38.71 Storing wcr puck in Collection Clamp to tube 2 @16:14:38.90 SC.reconfigure SCtight @16:14:38.96 SC.seek 2 @16:14:41.31 SC.reconfigure SCloose @16:14:42.13 Elbow.reconfigure TightElbow @16:14:42.18 Elbow.seek 2 @16:14:44.07 Forearm.reconfigure TightForearm @16:14:44.13 Forearm.seek 2 + 700 counts @16:14:45.17 SE.reconfigure SEpick @16:14:45.25 SE.seek beamBreak @16:14:47.44 SE.configure SEconfig @16:14:47.63 Elbow.reconfigure LimpElbow @16:14:47.68 Forearm.reconfigure LooseForearm @16:14:47.73 Forearm.seek 2 @16:14:48.07 Elbow.hold @16:14:48.15 Elbow.reconfigure TightElbow @16:14:48.27 SE.seek 9.07 Pucks below Top Plate @16:14:48.79 Forearm.force -20 @16:14:48.84 Forearm.seek 2 + 315 counts @16:14:49.18 Forearm.reconfigure TightForearm @16:14:49.24 Forearm.seek 2 + 700 counts @16:14:49.47 SE.seek 10.07 Pucks below Top Plate @16:14:50.14 Forearm.reconfigure LooseForearm @16:14:50.20 Forearm.seek :retract @16:14:51.15 Elbow.seek CC @16:14:52.78 Elbow.reconfigure LooseElbow @16:14:52.89 Forearm.seek CC @16:14:53.83 Hand.close @16:14:54.54 CC.seek :open @16:14:59.04 Forearm.seek :retract @16:14:59.99 Elbow.reconfigure TightElbow @16:15:00.04 Elbow.seek 2 @16:15:01.92 Elbow.reconfigure LimpElbow @16:15:01.98 Forearm.seek 2 @16:15:03.01 Elbow.hold @16:15:03.09 Elbow.reconfigure TightElbow @16:15:03.14 Hand.open @16:15:03.91 Forearm.force -20 @16:15:03.96 Forearm.seek 2 + 347 counts @16:15:04.31 Forearm.reconfigure TightForearm @16:15:04.36 Forearm.seek 2 + 700 counts @16:15:04.78 SE.seek :down @16:15:05.95 Forearm.reconfigure LooseForearm @16:15:06.01 Forearm.seek :retract @16:15:06.62 SE.coast @16:15:07.28 SC.coast @16:15:07.32 Starting finalPurge @16:15:07.34 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:15:07.42 Hand.open @16:15:07.80 Elbow.seek FlushPuck::Garage @16:15:09.67 Elbow.reconfigure LooseElbow @16:15:09.78 Forearm.seek :garage @16:15:10.78 Hand.close @16:15:11.54 Forearm.seek :retract @16:15:12.54 Elbow.reconfigure TightElbow @16:15:12.59 Elbow.seek CC @16:15:15.49 Elbow.reconfigure LooseElbow @16:15:15.59 Forearm.reconfigure TightForearm @16:15:15.64 Forearm.seek CC @16:15:16.94 CC.seek :closed @16:15:21.22 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.08 @16:15:21.30 Hand.open @16:15:21.98 Forearm.reconfigure LooseForearm @16:15:22.03 Forearm.seek :retract @16:15:23.03 Flushing the Collection Valve::Manifold @16:15:23.17 CSV.dial CSR @16:15:23.70 Pulling 5ml of CSR.flush @16:15:23.71 CSR.select :flush @16:15:23.84 CS.seek 5ml @16:15:37.69 delay 5 seconds @16:15:42.71 CSR.select :air @16:15:42.89 CS.reconfigure CSfast @16:15:42.94 CS.seek 7.501ml @16:15:49.77 CSV.dial :waste @16:15:51.14 CS.reconfigure CSconfig @16:15:51.19 CS.seek 0ml @16:16:11.31 Flushing lower manifold's waste1 port @16:16:11.45 CSV.dial :flush @16:16:11.91 CS.reconfigure CSfast @16:16:11.96 CS.seek 5ml @16:16:24.69 delay 5 seconds @16:16:29.70 CSV.dial :air @16:16:30.37 CS.seek 7ml @16:16:36.10 CSV.dial CTV @16:16:36.13
CTV.dial CSV @16:16:37.59 CBV.dial :waste1 @16:16:38.43 CS.seek 0ml @16:16:55.66 Flushing lower manifold's waste2b port @16:16:55.80 CSV.dial :flush @16:16:56.61 CS.seek 5ml @16:17:09.38 delay 5 seconds @16:17:14.40 CSV.dial :air @16:17:14.97 CS.seek 7.001ml @16:17:20.82 CSV.dial CTV @16:17:20.85
CTV.dial CSV @16:17:21.33 CBV.dial :waste2b @16:17:22.17 CS.seek 0ml @16:17:39.69 Flushing upper manifold @16:17:39.83 CSV.dial :flush @16:17:40.62 CS.seek 5ml @16:17:53.22 delay 5 seconds @16:17:58.23 CSV.dial :air @16:17:58.79 CS.seek 7ml @16:18:04.47 CSV.dial CBV @16:18:04.50
CBV.dial CSV @16:18:05.67 CTV.dial :air @16:18:07.06 CS.seek 0ml @16:18:24.16 delay 7 seconds @16:18:31.18 CS.seek 3ml @16:18:39.28 CSV.dial :waste @16:18:39.78 CS.seek 0ml @16:18:48.10 CSV.dial :flush @16:18:48.59 CS.seek 3ml @16:18:56.66 delay 5 seconds @16:19:01.67 CSV.dial :air @16:19:02.21 CS.seek 5ml @16:19:07.87 CSV.dial :air @16:19:07.90 CS.seek 0ml @16:19:20.44 delay 7 seconds @16:19:27.46 CS.seek 3ml @16:19:35.49 CSV.dial :waste @16:19:36.28 CS.seek 0ml @16:19:44.44 Disinfecting external Intake with mfbkill @16:19:44.58 CSV.dial CSR @16:19:46.00 Pulling 8ml of CSR.mfbkill @16:19:46.01 CSR.select :mfbkill @16:19:46.14 CS.seek 8ml @16:20:05.82 delay 5 seconds @16:20:10.84 CSR.select :air @16:20:10.98 CS.seek 10ml @16:20:16.66 CSV.dial CBV @16:20:16.69
CBV.dial CSV @16:20:17.77 CTV.dial :intake @16:20:18.57 Intake.select :open @16:20:18.69 CS.seek 2ml @16:20:39.12 Intake.select :close @16:20:39.24 CTV.dial :air @16:20:40.05 CS.reconfigure CSconfig @16:20:40.10 CS.seek 0ml @16:20:46.32 Flushing lower manifold's waste1 port @16:20:46.33 CSV.dial CSR @16:20:47.47 Pulling 4ml of CSR.mfbkill @16:20:47.48 CSR.select :mfbkill @16:20:47.61 CS.seek 4ml @16:20:58.82 delay 5 seconds @16:21:03.84 CSR.select :air @16:21:03.97 CS.reconfigure CSfast @16:21:04.02 CS.seek 10ml @16:21:19.07 CSV.dial CTV @16:21:19.67
CTV.dial CSV @16:21:21.16 CBV.dial :waste1 @16:21:21.67 CS.seek 0ml @16:21:46.13 Flushing lower manifold's waste2b port @16:21:46.14 CSV.dial CSR @16:21:46.70 Pulling 4ml of CSR.mfbkill @16:21:46.71 CSR.select :mfbkill @16:21:46.83 CS.reconfigure CSconfig @16:21:46.88 CS.seek 4ml @16:21:58.29 delay 5 seconds @16:22:03.31 CSR.select :air @16:22:03.44 CS.reconfigure CSfast @16:22:03.49 CS.seek 10ml @16:22:18.32 CSV.dial CTV @16:22:18.35
CTV.dial CSV @16:22:18.85 CBV.dial :waste2b @16:22:19.67 CS.seek 0ml @16:22:43.76 Killing upper manifold @16:22:43.77 CSV.dial CSR @16:22:44.32 Pulling 4ml of CSR.mfbkill @16:22:44.33 CSR.select :mfbkill @16:22:44.46 CS.reconfigure CSconfig @16:22:44.51 CS.seek 4ml @16:22:55.76 delay 5 seconds @16:23:00.77 CSR.select :air @16:23:00.90 CS.reconfigure CSfast @16:23:00.95 CS.seek 10ml @16:23:15.91 CSV.dial CBV @16:23:15.94
CBV.dial CSV @16:23:17.07 CTV.dial :air @16:23:18.47 CS.seek 0ml @16:23:42.57 delay 7 seconds @16:23:49.59 CS.seek 3ml @16:23:57.76 CSV.dial :waste @16:23:58.36 CS.seek 0ml @16:24:06.32 Flushing upper manifold @16:24:06.46 CSV.dial :flush @16:24:06.97 CS.seek 5ml @16:24:19.60 delay 5 seconds @16:24:24.61 CSV.dial :air @16:24:25.16 CS.seek 7ml @16:24:30.92 CSV.dial CBV @16:24:30.95
CBV.dial CSV @16:24:32.04 CTV.dial :air @16:24:32.06 CS.seek 0ml @16:24:49.17 delay 7 seconds @16:24:56.19 CS.seek 3ml @16:25:04.16 CSV.dial :waste @16:25:04.66 CS.seek 0ml @16:25:12.80 CSV.dial :flush @16:25:13.29 CS.seek 3ml @16:25:21.26 delay 5 seconds @16:25:26.27 CSV.dial :air @16:25:26.81 CS.seek 5ml @16:25:32.59 CSV.dial :air @16:25:32.62 CS.seek 0ml @16:25:45.14 delay 7 seconds @16:25:52.16 CS.seek 3ml @16:26:00.67 CSV.dial :waste @16:26:01.46 CS.seek 0ml @16:26:09.35 Flushing lower manifold's waste1 port @16:26:09.49 CSV.dial :flush @16:26:09.99 CS.seek 5ml @16:26:22.54 delay 5 seconds @16:26:27.56 CSV.dial :air @16:26:28.22 CS.seek 7ml @16:26:33.92 CSV.dial CTV @16:26:33.95
CTV.dial CSV @16:26:35.45 CBV.dial :waste1 @16:26:35.96 CS.seek 0ml @16:26:53.26 Flushing lower manifold's waste2b port @16:26:53.40 CSV.dial :flush @16:26:54.21 CS.seek 5ml @16:27:06.89 delay 5 seconds @16:27:11.91 CSV.dial :air @16:27:12.49 CS.seek 7.001ml @16:27:18.20 CSV.dial CTV @16:27:18.23
CTV.dial CSV @16:27:18.70 CBV.dial :waste2b @16:27:19.52 CS.seek 0ml @16:27:36.89 Disinfecting external Intake with kill @16:27:37.03 CSV.dial CSR @16:27:37.68 Pulling 8ml of CSR.kill @16:27:37.69 CSR.select :kill @16:27:37.82 CS.seek 8ml @16:27:57.29 delay 5 seconds @16:28:02.31 CSR.select :air @16:28:02.45 CS.seek 10ml @16:28:08.11 CSV.dial CBV @16:28:08.14
CBV.dial CSV @16:28:09.29 CTV.dial :intake @16:28:10.08 Intake.select :open @16:28:10.20 CS.seek 2ml @16:28:30.69 Intake.select :close @16:28:30.81 CTV.dial :air @16:28:31.62 CS.reconfigure CSconfig @16:28:31.67 CS.seek 0ml @16:28:37.72 CSV.dial CSR @16:28:38.87 Pulling 5ml of CSR.flush @16:28:38.88 CSR.select :flush @16:28:39.01 CS.seek 5ml @16:28:52.76 delay 5 seconds @16:28:57.78 CSR.select :air @16:28:57.92 CS.seek 10ml @16:29:11.72 CSV.dial CBV @16:29:12.92 CS.seek 0ml @16:29:39.39 Garaging FlushPuck in Collection Clamp @16:29:39.47 Elbow.reconfigure TightElbow @16:29:39.52 Elbow.seek CC @16:29:39.78 Elbow.reconfigure LooseElbow @16:29:39.89 Forearm.seek CC @16:29:40.90 Hand.close @16:29:41.55 CC.seek :open @16:29:46.53 Forearm.seek :retract @16:29:47.55 Elbow.reconfigure TightElbow @16:29:47.60 Elbow.seek :garage @16:29:50.65 Elbow.reconfigure LooseElbow @16:29:50.76 Forearm.reconfigure TightForearm @16:29:50.81 Forearm.seek :garage @16:29:51.99 Hand.open @16:29:52.74 Forearm.reconfigure LooseForearm @16:29:52.79 Forearm.seek :retract @16:29:53.78 Starting initialPurge @16:29:53.79 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:29:53.87 Hand.open @16:29:54.24 Elbow.reconfigure TightElbow @16:29:54.29 Elbow.seek FlushPuck::Garage @16:29:54.42 Elbow.reconfigure LooseElbow @16:29:54.53 Forearm.seek :garage @16:29:55.55 Hand.close @16:29:56.31 Forearm.seek :retract @16:29:57.37 Elbow.reconfigure TightElbow @16:29:57.42 Elbow.seek CC @16:30:00.31 Elbow.reconfigure LooseElbow @16:30:00.42 Forearm.reconfigure TightForearm @16:30:00.47 Forearm.seek CC @16:30:01.77 CC.seek :closed @16:30:06.03 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.09 @16:30:06.11 Hand.open @16:30:06.87 Forearm.reconfigure LooseForearm @16:30:06.92 Forearm.seek :retract @16:30:07.92 Flushing the Collection Valve::Manifold @16:30:08.06 CSV.dial CSR @16:30:09.31 Pulling 5ml of CSR.flush @16:30:09.32 CSR.select :flush @16:30:09.45 CS.seek 5ml @16:30:23.20 delay 5 seconds @16:30:28.22 CSR.select :air @16:30:28.40 CS.reconfigure CSfast @16:30:28.45 CS.seek 7.5ml @16:30:35.32 CSV.dial :waste @16:30:36.70 CS.reconfigure CSconfig @16:30:36.75 CS.seek 0ml @16:30:56.95 Purging external Intake @16:30:57.08 CSV.dial :flush @16:30:57.55 CS.reconfigure CSfast @16:30:57.60 CS.seek 10ml @16:31:21.61 delay 5 seconds @16:31:26.63 CSV.dial CBV @16:31:26.67
CBV.dial CSV @16:31:27.45 CTV.dial :intake @16:31:28.25 Intake.select :open @16:31:28.37 CS.seek 0ml @16:31:53.69 Intake.select :close @16:31:53.81 CTV.dial :air @16:31:54.61 Flushing upper manifold @16:31:55.32 CSV.dial :flush @16:31:56.12 CS.seek 5ml @16:32:08.77 delay 5 seconds @16:32:13.78 CSV.dial :air @16:32:14.38 CS.seek 7ml @16:32:20.05 CSV.dial CBV @16:32:20.08
CBV.dial CSV @16:32:21.17 CTV.dial :air @16:32:21.19 CS.seek 0ml @16:32:38.49 delay 7 seconds @16:32:45.51 CS.seek 3ml @16:32:53.44 CSV.dial :waste @16:32:53.93 CS.seek 0ml @16:33:02.23 CSV.dial :flush @16:33:02.73 CS.seek 3ml @16:33:10.67 delay 5 seconds @16:33:15.68 CSV.dial :air @16:33:16.24 CS.seek 5ml @16:33:22.02 CSV.dial :air @16:33:22.04 CS.seek 0ml @16:33:34.70 delay 7 seconds @16:33:41.72 CS.seek 3ml @16:33:49.76 CSV.dial :waste @16:33:50.57 CS.seek 0ml @16:33:58.71 Garaging FlushPuck in Collection Clamp @16:33:58.79 Elbow.reconfigure TightElbow @16:33:58.84 Elbow.seek CC @16:33:59.07 Elbow.reconfigure LooseElbow @16:33:59.18 Forearm.seek CC @16:34:00.17 Hand.close @16:34:00.80 CC.seek :open @16:34:05.18 Forearm.seek :retract @16:34:06.19 Elbow.reconfigure TightElbow @16:34:06.24 Elbow.seek :garage @16:34:10.07 Elbow.reconfigure LooseElbow @16:34:10.17 Forearm.reconfigure TightForearm @16:34:10.22 Forearm.seek :garage @16:34:11.39 Hand.open @16:34:12.25 Forearm.reconfigure LooseForearm @16:34:12.30 Forearm.seek :retract @16:34:13.32 Completed [mbaPhase] begun 14:00:25 @16:34:13.34 Preparing to sleep until Sat Oct 13 14:00:25 +0200 2018 @16:34:13.39 deferred until MFBPCR finish @16:34:13.42
Waiting for MFBPCR til 14:00CEST13-Oct-18 @16:34:13.43 Continuing at 14:00CEST13-Oct-18 @16:34:13.50 GoodNews.email "Then, in tube 3: mbaPhase\n/home/esp/esp2/mission/18tundeployv3.rb:123\n{1 => 22, 2 => 11, 3 => 11}\nCan@16:34:13, 14.3C, 70% humidity, 14.3psia, 13.092V, 0.960A, 0.960A avg, 12.57W",Subject: "Waiting until 14:00CEST13-Oct-18" @16:34:53.64 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @16:34:53.65 Retry #1 of 10 after 10:00 @16:44:53.68 GoodNews.email "Then, in tube 3: mbaPhase\n/home/esp/esp2/mission/18tundeployv3.rb:123\n{1 => 22, 2 => 11, 3 => 11}\nCan@16:34:13, 14.3C, 70% humidity, 14.3psia, 13.092V, 0.960A, 0.960A avg, 12.57W",Subject: "Waiting until 14:00CEST13-Oct-18" @16:45:28.77 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @16:45:28.79 Retry #2 of 10 after 10:00 @16:55:28.81 GoodNews.email "Then, in tube 3: mbaPhase\n/home/esp/esp2/mission/18tundeployv3.rb:123\n{1 => 22, 2 => 11, 3 => 11}\nCan@16:34:13, 14.3C, 70% humidity, 14.3psia, 13.092V, 0.960A, 0.960A avg, 12.57W",Subject: "Waiting until 14:00CEST13-Oct-18" @17:21:39.50 PCR.off @17:21:41.55 PCR for Ss completed -- closed /var/log/esp/Ss18oct1115h150ml.pcr @17:21:41.62 Cleaning PCR @17:21:41.63 AFV.select :carrierSolution @17:21:41.74 delay 2 seconds @17:21:43.77 AS.reconfigure ASconfig @17:21:43.82 AS.seek 0.5ml @17:21:54.13 delay 1 second @17:21:55.14 AFV.select :holdingCoil @17:21:55.25 ASV.dial :waste1 @17:21:55.63 ATV.dial :air3 @17:21:56.84 AS.reconfigure ASslow1 @17:21:56.89 AS.seek 0.51ml @17:21:59.18 delay 2 seconds @17:22:01.20 ATV.dial :bleach @17:22:01.81 AS.seek 0.81ml @17:23:02.41 delay 3 seconds @17:23:05.43 ATV.dial :air3 @17:23:05.96 delay 3 seconds @17:23:09.05 AS.seek 0.835ml @17:23:14.49 delay 3 seconds @17:23:17.51 ATV.dial :bleach @17:23:18.11 AS.seek 0.82ml @17:23:21.50 delay 3 seconds @17:23:24.52 ARV.dial :waste @17:23:25.11 delay 3 seconds @17:23:28.13 ATV.dial :ARV @17:23:28.98 AS.reconfigure ASconfig @17:23:29.03 AS.seek 0.47ml @17:23:49.45 AFV.select :carrierSolution @17:23:49.57 delay 2 seconds @17:23:51.60 AS.seek 1ml @17:24:22.33 delay 1 second @17:24:23.34 AFV.select :holdingCoil @17:24:23.45 delay 1 second @17:24:24.48 AS.reconfigure ASfast @17:24:24.53 AS.seek 0ml @17:24:51.82 AFV.select :carrierSolution @17:24:51.94 delay 2 seconds @17:24:53.97 AS.reconfigure ASconfig @17:24:54.02 AS.seek 1ml @17:25:51.79 delay 1 second @17:25:52.80 AFV.select :holdingCoil @17:25:52.92 delay 1 second @17:25:53.95 AS.reconfigure ASfast @17:25:54.00 AS.seek 0ml @17:26:21.34 AFV.select :carrierSolution @17:26:21.46 delay 2 seconds @17:26:23.49 AS.reconfigure ASconfig @17:26:23.54 AS.seek 1ml @17:27:21.32 delay 1 second @17:27:22.33 AFV.select :holdingCoil @17:27:22.44 delay 1 second @17:27:23.47 AS.reconfigure ASfast @17:27:23.52 AS.seek 0ml @17:27:50.86 ARV.dial between carrier and air1 @17:27:51.35 ATV.dial :air3 @17:27:52.56 AS.reconfigure ASslow1 @17:27:52.61 AS.seek 0.01ml @17:27:54.91 delay 2 seconds @17:27:57.42 ATV.dial :bleach @17:27:58.09 AS.seek 0.81ml @17:30:39.21 delay 3 seconds @17:30:42.23 ATV.dial :air3 @17:30:42.76 delay 3 seconds @17:30:45.85 AS.seek 0.835ml @17:30:51.13 delay 3 seconds @17:30:54.15 ATV.dial :bleach @17:30:54.75 AS.seek 0.82ml @17:30:58.05 delay 3 seconds @17:31:01.07 ATV.dial PCR @17:31:01.46 AS.reconfigure ASconfig @17:31:01.51 AS.seek :empty @17:31:48.98 PCR.program :bleachProfile @17:31:49.04 PCR program should complete in < 2:45 @17:31:49.05 PCR.run 10:00 @17:34:59.96 PCR.off @17:34:59.99 AFV.select :carrierSolution @17:35:00.11 delay 2 seconds @17:35:02.14 AS.seek 1ml @17:35:59.98 delay 1 second @17:36:00.99 AFV.select :holdingCoil @17:36:01.10 delay 1 second @17:36:02.13 AS.reconfigure ASfast @17:36:02.18 AS.seek 0ml @17:36:29.52 delay 3 seconds @17:36:32.54 AFV.select :carrierSolution @17:36:32.65 delay 2 seconds @17:36:34.68 AS.reconfigure ASconfig @17:36:34.74 AS.seek 1ml @17:37:32.52 delay 1 second @17:37:33.53 AFV.select :holdingCoil @17:37:33.64 delay 1 second @17:37:34.67 AS.reconfigure ASfast @17:37:34.73 AS.seek 0ml @17:38:02.07 delay 3 seconds @17:38:05.09 AFV.select :carrierSolution @17:38:05.20 delay 2 seconds @17:38:07.23 AS.reconfigure ASconfig @17:38:07.29 AS.seek 1ml @17:39:05.04 delay 1 second @17:39:06.05 AFV.select :holdingCoil @17:39:06.16 delay 1 second @17:39:07.19 AS.reconfigure ASfast @17:39:07.24 AS.seek 0ml @17:39:34.60 delay 3 seconds @17:39:37.61 AFV.select :carrierSolution @17:39:37.73 delay 2 seconds @17:39:39.76 AS.reconfigure ASconfig @17:39:39.82 AS.seek 1ml @17:40:37.57 delay 1 second @17:40:38.58 AFV.select :holdingCoil @17:40:38.69 delay 1 second @17:40:39.72 AS.reconfigure ASfast @17:40:39.77 AS.seek 0ml @17:41:07.12 delay 3 seconds @17:41:10.14 AFV.select :carrierSolution @17:41:10.25 delay 2 seconds @17:41:12.28 AS.reconfigure ASconfig @17:41:12.34 AS.seek 1ml @17:42:10.14 delay 1 second @17:42:11.15 AFV.select :holdingCoil @17:42:11.26 delay 1 second @17:42:12.29 AS.reconfigure ASnorm @17:42:12.34 AS.seek 0ml @17:43:03.05 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1117h150ml.pcr @17:43:03.07 Building PCR Slug from ASV with MM2 mix, PP4 primer @17:43:03.09 Priming with carrier @17:43:03.10 ATV.dial ARV @17:43:03.67 ARV.dial :carrier @17:43:04.02 AS.seek 0.5ml @17:43:29.58 AFV.select :carrierSolution @17:43:29.70 delay 2 seconds @17:43:31.73 AS.reconfigure ASconfig @17:43:31.78 AS.seek 1ml @17:44:00.81 delay 1 second @17:44:01.83 AFV.select :holdingCoil @17:44:01.94 delay 1 second @17:44:02.96 ATV.dial :waste @17:44:04.51 AS.reconfigure ASnorm @17:44:04.56 AS.seek :empty @17:44:55.30 AFV.select :carrierSolution @17:44:55.42 delay 2 seconds @17:44:57.45 AS.reconfigure ASconfig @17:44:57.50 AS.seek 0.5ml @17:45:26.61 delay 1 second @17:45:27.62 AFV.select :holdingCoil @17:45:27.73 Building Slug @17:45:27.74 ATV.dial :ARV @17:45:29.25 delay 2 seconds @17:45:31.27 ARV.dial :air1 @17:45:31.77 delay 2 seconds @17:45:33.85 AS.reconfigure ASslow2 @17:45:33.91 AS.seek 0.506ml @17:45:40.07 delay 2 seconds @17:45:42.09 ARV.dial :MM2 @17:45:43.50 delay 2 seconds @17:45:46.12 Adding MM2 @17:45:46.19 AS.seek 0.524ml @17:46:04.61 delay 25 seconds @17:46:29.63 ARV.dial :PP4 @17:46:30.01 Adding PP4 @17:46:30.02 delay 2 seconds @17:46:32.11 AS.seek 0.53ml @17:46:38.51 delay 10 seconds @17:46:48.53 ARV.dial :air3 @17:46:48.92 adding ARV air @17:46:48.94 delay 2 seconds @17:46:51.02 AS.seek 0.536ml @17:46:57.45 delay 5 seconds @17:47:02.47 ARV.dial :carrier @17:47:03.65 delay 7 seconds @17:47:10.74 AS.seek 0.594ml @17:48:09.68 delay 5 seconds @17:48:14.71 ATV.dial :ASV @17:48:15.09 delay 2 seconds @17:48:17.18 AS.seek 0.6ml @17:48:23.43 delay 4 seconds @17:48:27.45 ATV.dial ARV @17:48:27.98 delay 2 seconds @17:48:30.00 ARV.dial :carrier @17:48:30.02 delay 2 seconds @17:48:32.11 AS.seek 0.65ml @17:49:22.69 delay 4 seconds @17:49:26.71 ATV.dial PCR @17:49:27.29 ASV.dial :waste1 @17:49:27.30 ARV.dial :air1 @17:49:27.78 Positioning Slug by 0.324ml @17:49:27.87 AS.seek 0.326ml @17:54:53.78 delay 5 seconds @17:54:58.80 ATV.dial ARV @17:54:59.55 ASV.dial :PCRlock @17:55:00.02 delay 5 seconds @17:55:05.05 Running Tt1 Profile60C300 thermocycling @17:55:05.06 PCR.program Profile60C300 @17:55:05.22 Profile60C300 should complete in < 1:45:21 @17:55:05.23 PCR.run 2:16:41.25 @19:16:57.91 PCR.off @19:16:59.49 PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1117h150ml.pcr @19:16:59.57 Cleaning PCR @19:16:59.58 AFV.select :carrierSolution @19:16:59.69 delay 2 seconds @19:17:01.72 AS.reconfigure ASconfig @19:17:01.77 AS.seek 0.5ml @19:17:12.06 delay 1 second @19:17:13.08 AFV.select :holdingCoil @19:17:13.19 ASV.dial :waste1 @19:17:13.56 ATV.dial :air3 @19:17:14.76 AS.reconfigure ASslow1 @19:17:14.82 AS.seek 0.51ml @19:17:17.09 delay 2 seconds @19:17:19.11 ATV.dial :bleach @19:17:19.71 AS.seek 0.81ml @19:18:20.29 delay 3 seconds @19:18:23.31 ATV.dial :air3 @19:18:23.84 delay 3 seconds @19:18:26.92 AS.seek 0.835ml @19:18:32.19 delay 3 seconds @19:18:35.22 ATV.dial :bleach @19:18:35.82 AS.seek 0.82ml @19:18:39.11 delay 3 seconds @19:18:42.12 ARV.dial :waste @19:18:42.70 delay 3 seconds @19:18:45.72 ATV.dial :ARV @19:18:46.56 AS.reconfigure ASconfig @19:18:46.61 AS.seek 0.47ml @19:19:07.00 AFV.select :carrierSolution @19:19:07.12 delay 2 seconds @19:19:09.15 AS.seek 1ml @19:19:39.89 delay 1 second @19:19:40.91 AFV.select :holdingCoil @19:19:41.03 delay 1 second @19:19:42.06 AS.reconfigure ASfast @19:19:42.11 AS.seek 0ml @19:20:09.44 AFV.select :carrierSolution @19:20:09.56 delay 2 seconds @19:20:11.59 AS.reconfigure ASconfig @19:20:11.64 AS.seek 1ml @19:21:09.42 delay 1 second @19:21:10.43 AFV.select :holdingCoil @19:21:10.54 delay 1 second @19:21:11.57 AS.reconfigure ASfast @19:21:11.63 AS.seek 0ml @19:21:38.96 AFV.select :carrierSolution @19:21:39.08 delay 2 seconds @19:21:41.11 AS.reconfigure ASconfig @19:21:41.16 AS.seek 1ml @19:22:38.88 delay 1 second @19:22:39.90 AFV.select :holdingCoil @19:22:40.01 delay 1 second @19:22:41.04 AS.reconfigure ASfast @19:22:41.09 AS.seek 0ml @19:23:08.43 ARV.dial between carrier and air1 @19:23:08.92 ATV.dial :air3 @19:23:10.12 AS.reconfigure ASslow1 @19:23:10.18 AS.seek 0.01ml @19:23:12.51 delay 2 seconds @19:23:14.52 ATV.dial :bleach @19:23:15.13 AS.seek 0.81ml @19:25:56.28 delay 3 seconds @19:25:59.30 ATV.dial :air3 @19:25:59.83 delay 3 seconds @19:26:02.92 AS.seek 0.835ml @19:26:08.25 delay 3 seconds @19:26:11.27 ATV.dial :bleach @19:26:11.87 AS.seek 0.82ml @19:26:15.17 delay 3 seconds @19:26:18.18 ATV.dial PCR @19:26:18.58 AS.reconfigure ASconfig @19:26:18.63 AS.seek :empty @19:27:06.10 PCR.program :bleachProfile @19:27:06.17 PCR program should complete in < 2:45 @19:27:06.18 PCR.run 10:00 @19:30:17.09 PCR.off @19:30:17.11 AFV.select :carrierSolution @19:30:17.23 delay 2 seconds @19:30:19.26 AS.seek 1ml @19:31:17.08 delay 1 second @19:31:18.09 AFV.select :holdingCoil @19:31:18.20 delay 1 second @19:31:19.23 AS.reconfigure ASfast @19:31:19.28 AS.seek 0ml @19:31:46.63 delay 3 seconds @19:31:49.65 AFV.select :carrierSolution @19:31:49.76 delay 2 seconds @19:31:51.79 AS.reconfigure ASconfig @19:31:51.85 AS.seek 1ml @19:32:49.60 delay 1 second @19:32:50.61 AFV.select :holdingCoil @19:32:50.72 delay 1 second @19:32:51.75 AS.reconfigure ASfast @19:32:51.80 AS.seek 0ml @19:33:19.14 delay 3 seconds @19:33:22.16 AFV.select :carrierSolution @19:33:22.27 delay 2 seconds @19:33:24.30 AS.reconfigure ASconfig @19:33:24.35 AS.seek 1ml @19:34:22.10 delay 1 second @19:34:23.12 AFV.select :holdingCoil @19:34:23.23 delay 1 second @19:34:24.26 AS.reconfigure ASfast @19:34:24.31 AS.seek 0ml @19:34:51.66 delay 3 seconds @19:34:54.68 AFV.select :carrierSolution @19:34:54.79 delay 2 seconds @19:34:56.82 AS.reconfigure ASconfig @19:34:56.88 AS.seek 1ml @19:35:54.71 delay 1 second @19:35:55.72 AFV.select :holdingCoil @19:35:55.83 delay 1 second @19:35:56.86 AS.reconfigure ASfast @19:35:56.91 AS.seek 0ml @19:36:24.27 delay 3 seconds @19:36:27.29 AFV.select :carrierSolution @19:36:27.40 delay 2 seconds @19:36:29.43 AS.reconfigure ASconfig @19:36:29.48 AS.seek 1ml @19:37:27.21 delay 1 second @19:37:28.23 AFV.select :holdingCoil @19:37:28.34 delay 1 second @19:37:29.37 AS.reconfigure ASnorm @19:37:29.42 AS.seek 0ml @19:38:20.12 Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1119h150ml.pcr @19:38:20.14 Building PCR Slug from ASV with MM1 mix, PP1 primer @19:38:20.15 Priming with carrier @19:38:20.16 ATV.dial ARV @19:38:20.74 ARV.dial :carrier @19:38:21.09 AS.seek 0.5ml @19:38:46.63 AFV.select :carrierSolution @19:38:46.75 delay 2 seconds @19:38:48.78 AS.reconfigure ASconfig @19:38:48.83 AS.seek 1ml @19:39:17.91 delay 1 second @19:39:18.92 AFV.select :holdingCoil @19:39:19.03 delay 1 second @19:39:20.05 ATV.dial :waste @19:39:21.58 AS.reconfigure ASnorm @19:39:21.63 AS.seek :empty @19:40:12.37 AFV.select :carrierSolution @19:40:12.49 delay 2 seconds @19:40:14.52 AS.reconfigure ASconfig @19:40:14.57 AS.seek 0.5ml @19:40:43.72 delay 1 second @19:40:44.73 AFV.select :holdingCoil @19:40:44.84 Building Slug @19:40:44.85 ATV.dial :ARV @19:40:46.36 delay 2 seconds @19:40:48.38 ARV.dial :air1 @19:40:48.77 delay 2 seconds @19:40:50.85 AS.reconfigure ASslow2 @19:40:50.90 AS.seek 0.506ml @19:40:57.07 delay 2 seconds @19:40:59.09 ARV.dial :MM1 @19:40:59.66 delay 2 seconds @19:41:01.69 Adding MM1 @19:41:02.35 AS.seek 0.524ml @19:41:20.74 delay 25 seconds @19:41:45.76 ARV.dial :PP1 @19:41:46.15 Adding PP1 @19:41:46.16 delay 2 seconds @19:41:48.25 AS.seek 0.53ml @19:41:54.54 delay 10 seconds @19:42:04.56 ARV.dial :air1 @19:42:04.94 adding ARV air @19:42:04.95 delay 2 seconds @19:42:07.04 AS.seek 0.536ml @19:42:13.44 delay 5 seconds @19:42:18.47 ARV.dial :carrier @19:42:18.86 delay 7 seconds @19:42:25.95 AS.seek 0.594ml @19:43:24.76 delay 5 seconds @19:43:29.78 ATV.dial :ASV @19:43:30.16 delay 2 seconds @19:43:32.25 AS.seek 0.6ml @19:43:38.57 delay 4 seconds @19:43:42.59 ATV.dial ARV @19:43:43.21 delay 2 seconds @19:43:45.24 ARV.dial :carrier @19:43:45.25 delay 2 seconds @19:43:47.34 AS.seek 0.65ml @19:44:37.90 delay 4 seconds @19:44:41.92 ATV.dial PCR @19:44:42.52 ASV.dial :waste1 @19:44:42.53 ARV.dial :air1 @19:44:42.92 Positioning Slug by 0.324ml @19:44:43.01 AS.seek 0.326ml @19:50:09.05 delay 5 seconds @19:50:14.07 ATV.dial ARV @19:50:14.91 ASV.dial :PCRlock @19:50:15.27 delay 5 seconds @19:50:20.30 Running IPc ProfileIPc thermocycling @19:50:20.31 PCR.program ProfileIPc @19:50:20.46 ProfileIPc should complete in < 1:50:49 @19:50:20.47 PCR.run 2:23:31.25 @21:14:32.35 PCR.off @21:14:34.95 PCR for IPc completed -- closed /var/log/esp/IPc18oct1119h150ml.pcr @21:14:34.97 Full MFB Decontamination @21:14:34.98 Decon extraction column and decon AIV @21:14:35.00 SPE.hold 2:46:40,55C @21:14:35.05 ASV.dial :SPEdelivery @21:14:35.66 delay 20 seconds @21:14:55.68 AFV.select :carrierSolution @21:14:55.79 delay 2 seconds @21:14:57.82 AS.reconfigure ASconfig @21:14:57.87 AS.seek 0.5ml @21:15:08.16 delay 1 second @21:15:09.17 AFV.select :holdingCoil @21:15:09.28 ATV.dial :air1 @21:15:11.20 AS.reconfigure ASslow1 @21:15:11.25 AS.seek 0.52ml @21:15:15.53 ATV.dial :ASV @21:15:17.04 ASV.dial :SPErecovery @21:15:17.69 AS.seek 0.62ml @21:15:38.06 ATV.dial :waste @21:15:39.91 AS.reconfigure ASnorm @21:15:40.51 AS.seek :empty @21:16:12.14 AFV.select :holdingCoil @21:16:12.16 ATV.dial :waste @21:16:12.18 AS.reconfigure ASconfig @21:16:12.23 AS.seek 0ml @21:16:12.53 AFV.select :carrierSolution @21:16:12.65 delay 2 seconds @21:16:14.68 AS.seek 0.5ml @21:16:43.71 delay 1 second @21:16:44.72 AFV.select :holdingCoil @21:16:44.83 ATV.dial :air3 @21:16:45.51 AS.reconfigure ASslow1 @21:16:45.56 AS.seek 0.52ml @21:16:49.82 ATV.dial :air3 @21:16:49.91 AS.seek 0.53ml @21:16:52.19 delay 2 seconds @21:16:54.20 ATV.dial :bleach @21:16:54.67 AS.seek 0.71ml @21:17:31.15 delay 3 seconds @21:17:34.17 ATV.dial :air3 @21:17:34.71 delay 3 seconds @21:17:37.80 AS.seek 0.735ml @21:17:43.02 delay 3 seconds @21:17:46.04 ATV.dial :bleach @21:17:46.66 AS.seek 0.72ml @21:17:49.96 delay 3 seconds @21:17:52.99 ATV.dial ASV @21:17:53.99 ASV.dial :SPErecovery @21:17:54.07 AS.seek 0.365ml @21:19:05.73 ATV.dial :waste @21:19:07.46 AS.reconfigure ASnorm @21:19:07.51 AS.seek :empty @21:19:26.26 ATV.dial :air3 @21:19:26.94 AS.reconfigure ASslow1 @21:19:26.99 AS.seek 0.009ml @21:19:29.34 delay 2 seconds @21:19:31.35 ATV.dial :bleach @21:19:31.81 AS.seek 0.579ml @21:21:26.70 delay 3 seconds @21:21:29.72 ATV.dial :air3 @21:21:30.26 delay 3 seconds @21:21:33.35 AS.seek 0.604ml @21:21:38.66 delay 3 seconds @21:21:41.68 ATV.dial :bleach @21:21:42.28 AS.seek 0.589ml @21:21:45.56 delay 3 seconds @21:21:48.58 ATV.dial :ASV @21:21:49.66 ASV.dial :wasteR @21:21:50.04 AS.seek 0ml @21:23:48.87 delay 1:00 @21:24:48.89 ATV.dial ASV @21:24:48.91 AFV.select :carrierSolution @21:24:49.01 delay 2 seconds @21:24:51.04 AS.reconfigure ASconfig @21:24:51.09 AS.seek 1ml @21:25:48.84 delay 1 second @21:25:49.85 AFV.select :holdingCoil @21:25:49.96 delay 1 second @21:25:50.98 ASV.dial :wasteR @21:25:51.00 AS.reconfigure ASslow1 @21:25:51.05 AS.seek 0ml @21:29:12.45 delay 10 seconds @21:29:22.47 ATV.dial :air3 @21:29:23.88 AS.seek 0.01ml @21:29:26.17 delay 2 seconds @21:29:28.18 ATV.dial :bleach @21:29:28.78 AS.seek 0.58ml @21:31:23.68 delay 3 seconds @21:31:26.70 ATV.dial :air3 @21:31:27.23 delay 3 seconds @21:31:30.32 AS.seek 0.605ml @21:31:35.62 delay 3 seconds @21:31:38.64 ATV.dial :bleach @21:31:39.24 AS.seek 0.59ml @21:31:42.53 delay 3 seconds @21:31:45.57 ATV.dial ASV @21:31:46.53 ASV.dial :SPEdelivery @21:31:46.90 AS.seek 0ml @21:33:45.78 delay 1:00 @21:34:45.80 AFV.select :carrierSolution @21:34:45.91 delay 2 seconds @21:34:47.94 AS.reconfigure ASconfig @21:34:47.99 AS.seek 1ml @21:35:45.79 delay 1 second @21:35:46.80 AFV.select :holdingCoil @21:35:46.91 delay 1 second @21:35:47.93 ATV.dial ASV @21:35:47.95 ASV.dial :SPEdelivery @21:35:47.97 AS.reconfigure ASslow1 @21:35:48.60 AS.seek 0ml @21:39:10.00 delay 10 seconds @21:39:20.02 ATV.dial :air2 @21:39:20.80 AS.reconfigure ASslow0 @21:39:20.86 AS.seek 0.8ml @21:40:41.65 delay 3 seconds @21:40:44.67 ATV.dial :ASV @21:40:45.61 ASV.dial :SPEdelivery @21:40:45.63 AS.reconfigure ASslow1 @21:40:45.68 AS.seek 0.03ml @21:43:20.83 delay 20 seconds @21:43:40.86 ATV.dial :waste,via: :air3 @21:43:42.45 AS.reconfigure ASconfig @21:43:42.50 AS.seek :empty @21:43:44.58 SPE.coast @21:43:44.67 Removing Bleach from Mixing Coil @21:43:44.68 AFV.select :carrierSolution @21:43:44.79 delay 2 seconds @21:43:46.82 AS.seek 0.5ml @21:44:15.94 delay 1 second @21:44:16.95 AFV.select :holdingCoil @21:44:17.06 ATV.dial :ASV @21:44:18.74 ASV.dial :SPErecovery @21:44:19.37 AS.reconfigure ASslow1 @21:44:19.43 AS.seek 0.73ml @21:45:05.90 ASV.dial :wasteD @21:45:06.47 AS.reconfigure ASnorm @21:45:06.53 AS.seek 0ml @21:45:43.65 AFV.select :carrierSolution @21:45:43.77 delay 2 seconds @21:45:45.80 AS.reconfigure ASconfig @21:45:45.85 AS.seek 0.5ml @21:46:14.91 delay 1 second @21:46:15.92 AFV.select :holdingCoil @21:46:16.03 ATV.dial ASV @21:46:16.04 ASV.dial :SPErecovery @21:46:16.66 AS.reconfigure ASslow0 @21:46:16.71 AS.seek 0.3ml @21:46:37.15 ATV.dial :air2 @21:46:38.13 AS.reconfigure ASslow1 @21:46:38.18 AS.seek 0.32ml @21:46:42.50 ATV.dial ASV @21:46:43.49 AS.reconfigure ASslow0 @21:46:43.54 AS.seek 0.57ml @21:47:09.01 ASV.dial :wasteD @21:47:09.66 AS.reconfigure ASnorm @21:47:09.72 AS.seek 0ml @21:47:38.77 AFV.select :carrierSolution @21:47:38.89 delay 2 seconds @21:47:40.92 AS.reconfigure ASconfig @21:47:40.97 AS.seek 0.5ml @21:48:10.00 delay 1 second @21:48:11.01 AFV.select :holdingCoil @21:48:11.12 ATV.dial ASV @21:48:11.13 ASV.dial :SPErecovery @21:48:11.76 AS.reconfigure ASslow0 @21:48:11.81 AS.seek 0.3ml @21:48:32.24 ATV.dial :air2 @21:48:33.24 AS.reconfigure ASslow1 @21:48:33.29 AS.seek 0.32ml @21:48:37.59 ATV.dial ASV @21:48:38.58 AS.reconfigure ASslow0 @21:48:38.64 AS.seek 0.57ml @21:49:04.10 ASV.dial :wasteD @21:49:04.66 AS.reconfigure ASnorm @21:49:04.72 AS.seek 0ml @21:49:33.80 ATV.dial :air2 @21:49:34.72 AS.reconfigure ASslow0 @21:49:34.78 AS.seek 0.3ml @21:50:05.29 ATV.dial :ASV @21:50:06.22 ASV.dial :wasteD @21:50:06.24 AS.reconfigure ASconfig @21:50:06.28 AS.seek 0.05ml @21:50:20.96 ATV.dial :waste @21:50:22.67 AS.seek 0ml @21:50:25.85 ASV.dial :SPEdelivery @21:50:26.79 deconLysateINT @21:50:26.80 ATV.dial :air3 @21:50:27.46 AS.reconfigure ASslow1 @21:50:27.51 AS.seek 0.01ml @21:50:29.85 delay 2 seconds @21:50:31.88 ATV.dial :bleach @21:50:32.34 AS.seek 0.96ml @21:53:43.64 delay 3 seconds @21:53:46.66 ATV.dial :air3 @21:53:47.19 delay 3 seconds @21:53:50.29 AS.seek 0.985ml @21:53:55.59 delay 3 seconds @21:53:58.61 ATV.dial :bleach @21:53:59.21 AS.seek 0.97ml @21:54:02.53 delay 3 seconds @21:54:05.56 ATV.dial AIV @21:54:06.72 AIV.dial :coilToMFB @21:54:06.73 AWV.select :waste @21:54:06.74 AFV.select :carrierSolution @21:54:06.86 delay 2 seconds @21:54:08.89 AS.reconfigure ASconfig @21:54:08.94 AS.seek 1ml @21:54:10.97 delay 1 second @21:54:11.98 AFV.select :holdingCoil @21:54:12.09 delay 1 second @21:54:13.12 AS.reconfigure ASslow0 @21:54:13.17 AS.seek :empty @21:55:54.09 AFV.select :carrierSolution @21:55:54.21 delay 2 seconds @21:55:56.24 AS.reconfigure ASconfig @21:55:56.29 AS.seek 1ml @21:56:54.08 delay 1 second @21:56:55.09 AFV.select :holdingCoil @21:56:55.20 delay 1 second @21:56:56.23 AS.reconfigure ASslow0 @21:56:56.28 AS.seek :empty @21:58:37.24 AFV.select :carrierSolution @21:58:37.36 delay 2 seconds @21:58:39.39 AS.reconfigure ASconfig @21:58:39.44 AS.seek 1ml @21:59:37.24 delay 1 second @21:59:38.25 AFV.select :holdingCoil @21:59:38.36 delay 1 second @21:59:39.39 AS.reconfigure ASslow0 @21:59:39.44 AS.seek :empty @22:01:20.43 ATV.dial :air2 @22:01:21.07 AS.reconfigure ASslow1 @22:01:21.12 AS.seek 0.009ml @22:01:23.40 AS.reconfigure ASslow0 @22:01:23.45 AS.seek 1ml @22:03:03.42 ATV.dial AIV @22:03:04.16 AS.seek 0.02ml @22:04:43.04 ATV.dial :waste @22:04:44.57 AS.seek 0ml @22:04:46.87 AWV.select :air @22:04:47.04 Cleaning PCR @22:04:47.05 AFV.select :carrierSolution @22:04:47.16 delay 2 seconds @22:04:49.19 AS.reconfigure ASconfig @22:04:49.24 AS.seek 0.5ml @22:05:18.27 delay 1 second @22:05:19.28 AFV.select :holdingCoil @22:05:19.39 ASV.dial :waste1 @22:05:19.83 ATV.dial :air3 @22:05:20.48 AS.reconfigure ASslow1 @22:05:20.53 AS.seek 0.51ml @22:05:22.85 delay 2 seconds @22:05:24.87 ATV.dial :bleach @22:05:25.33 AS.seek 0.81ml @22:06:25.97 delay 3 seconds @22:06:28.99 ATV.dial :air3 @22:06:29.52 delay 3 seconds @22:06:32.61 AS.seek 0.835ml @22:06:37.95 delay 3 seconds @22:06:40.98 ATV.dial :bleach @22:06:41.58 AS.seek 0.82ml @22:06:44.88 delay 3 seconds @22:06:47.90 ARV.dial :waste @22:06:48.49 delay 3 seconds @22:06:51.51 ATV.dial :ARV @22:06:52.35 AS.reconfigure ASconfig @22:06:52.41 AS.seek 0.47ml @22:07:12.81 AFV.select :carrierSolution @22:07:12.93 delay 2 seconds @22:07:14.96 AS.seek 1ml @22:07:45.71 delay 1 second @22:07:46.72 AFV.select :holdingCoil @22:07:46.83 delay 1 second @22:07:47.86 AS.reconfigure ASfast @22:07:47.91 AS.seek 0ml @22:08:15.34 AFV.select :carrierSolution @22:08:15.46 delay 2 seconds @22:08:17.49 AS.reconfigure ASconfig @22:08:17.54 AS.seek 1ml @22:09:15.32 delay 1 second @22:09:16.33 AFV.select :holdingCoil @22:09:16.44 delay 1 second @22:09:17.47 AS.reconfigure ASfast @22:09:17.52 AS.seek 0ml @22:09:44.86 AFV.select :carrierSolution @22:09:44.98 delay 2 seconds @22:09:47.01 AS.reconfigure ASconfig @22:09:47.06 AS.seek 1ml @22:10:44.85 delay 1 second @22:10:45.86 AFV.select :holdingCoil @22:10:45.97 delay 1 second @22:10:47.00 AS.reconfigure ASfast @22:10:47.05 AS.seek 0ml @22:11:14.39 ARV.dial between carrier and air1 @22:11:14.88 ATV.dial :air3 @22:11:16.67 AS.reconfigure ASslow1 @22:11:16.72 AS.seek 0.01ml @22:11:19.05 delay 2 seconds @22:11:21.06 ATV.dial :bleach @22:11:21.77 AS.seek 0.81ml @22:14:02.98 delay 3 seconds @22:14:06.00 ATV.dial :air3 @22:14:06.52 delay 3 seconds @22:14:09.61 AS.seek 0.835ml @22:14:14.91 delay 3 seconds @22:14:17.93 ATV.dial :bleach @22:14:18.53 AS.seek 0.82ml @22:14:21.77 delay 3 seconds @22:14:24.79 ATV.dial PCR @22:14:25.18 AS.reconfigure ASconfig @22:14:25.23 AS.seek :empty @22:15:12.70 PCR.program :bleachProfile @22:15:12.75 PCR program should complete in < 2:45 @22:15:12.77 PCR.run 10:00 @22:18:32.77 PCR.off @22:18:32.79 AFV.select :carrierSolution @22:18:32.91 delay 2 seconds @22:18:34.94 AS.seek 1ml @22:19:32.78 delay 1 second @22:19:33.79 AFV.select :holdingCoil @22:19:33.90 delay 1 second @22:19:34.93 AS.reconfigure ASfast @22:19:34.98 AS.seek 0ml @22:20:02.34 delay 3 seconds @22:20:05.36 AFV.select :carrierSolution @22:20:05.47 delay 2 seconds @22:20:07.50 AS.reconfigure ASconfig @22:20:07.56 AS.seek 1ml @22:21:05.34 delay 1 second @22:21:06.35 AFV.select :holdingCoil @22:21:06.46 delay 1 second @22:21:07.49 AS.reconfigure ASfast @22:21:07.54 AS.seek 0ml @22:21:34.90 delay 3 seconds @22:21:37.92 AFV.select :carrierSolution @22:21:38.03 delay 2 seconds @22:21:40.06 AS.reconfigure ASconfig @22:21:40.11 AS.seek 1ml @22:22:37.90 delay 1 second @22:22:38.91 AFV.select :holdingCoil @22:22:39.02 delay 1 second @22:22:40.05 AS.reconfigure ASfast @22:22:40.10 AS.seek 0ml @22:23:07.45 delay 3 seconds @22:23:10.47 AFV.select :carrierSolution @22:23:10.58 delay 2 seconds @22:23:12.61 AS.reconfigure ASconfig @22:23:12.67 AS.seek 1ml @22:24:10.46 delay 1 second @22:24:11.47 AFV.select :holdingCoil @22:24:11.58 delay 1 second @22:24:12.61 AS.reconfigure ASfast @22:24:12.66 AS.seek 0ml @22:24:40.01 delay 3 seconds @22:24:43.03 AFV.select :carrierSolution @22:24:43.14 delay 2 seconds @22:24:45.17 AS.reconfigure ASconfig @22:24:45.23 AS.seek 1ml @22:25:43.01 delay 1 second @22:25:44.02 AFV.select :holdingCoil @22:25:44.13 delay 1 second @22:25:45.16 AS.reconfigure ASnorm @22:25:45.21 AS.seek 0ml @22:26:35.90 AFV.select :carrierSolution @22:26:36.01 delay 2 seconds @22:26:38.04 AS.reconfigure ASconfig @22:26:38.09 AS.seek 1ml @22:27:35.89 delay 1 second @22:27:36.90 AFV.select :holdingCoil @22:27:37.01 delay 1 second @22:27:38.03 ATV.dial :waste @22:27:39.17 AS.reconfigure ASnorm @22:27:39.22 AS.seek 0.5ml @22:28:04.75 ATV.dial :waste2 @22:28:05.93 AS.seek 0ml @22:28:31.45 AS.coast @22:28:31.49 SS.coast @22:28:31.53 CC.coast @22:28:31.57 CS.coast @22:28:31.61 PC.coast @22:28:31.65 PS.coast @22:28:31.69 Forearm.coast @22:28:31.73 Elbow.coast @22:28:32.13 Gate.power :core,:OFF @22:28:32.14 Gate.power :raw,:OFF @22:28:32.15 Shutdown @22:28:32.17 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @22:28:53.20 Errno::ETIMEDOUT in Qmail -- Connection timed out - connect(2) @22:28:53.21 Retry #1 of 10 after 10:00 @22:38:53.24 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @22:39:33.35 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @22:39:33.36 Retry #2 of 10 after 10:00 @22:49:33.39 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @05:24:28.02CEST12-Oct-18 Session Begun @05:24:29.35 -> can @05:24:39.77 -> quit @05:24:39.78 Session Ended @08:40:52.37 Session Begun @08:40:55.81 -> can @08:41:08.20 -> exit @08:41:08.22 Session Ended @14:00:25.59CEST13-Oct-18
Powering core bus @14:00:30.69 Gate.power :raw,:ON @14:00:31.35 SPE.coast @14:00:31.42 SPE.configure SPEconfig @14:00:31.51 AS.configure ASconfig @14:00:31.61 SS.configure SSconfig @14:00:31.71 CC.configure CCconfig @14:00:31.80 CS.configure CSconfig @14:00:31.90 PC.configure PCconfig @14:00:32.46 PS.configure PSconfig @14:00:32.66 Forearm.configure LooseForearm @14:00:32.76 Elbow.configure LooseElbow @14:00:32.88 SC.configure SCloose @14:00:32.98 SE.configure SEconfig @14:00:33.03 CH.coast @14:00:33.09 CH.configure CHconfig @14:00:33.12 PH.coast @14:00:33.18 PH.configure PHconfig @14:00:33.22 PCR.off @14:00:33.25 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:33.28 PCR.caltemp= 2.7073,0.0373,0 @14:00:33.31 All dwarf controllers running firmware version 3.75 @14:00:33.43 Valve.reset @14:00:33.60 PCR.off @14:00:33.63 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:33.66 PCR.caltemp= 2.7073,0.0373,0 @14:00:33.91 PSV.dial :air @14:00:33.92 Intake.select :close @14:00:33.95 Exhaust.select :close @14:00:33.98 CTV.dial :air @14:00:34.15 CSV.dial :waste1 @14:00:34.18
Forearm.reconfigure LooseForearm @14:00:34.30 SSV.dial :spit,avoiding: :exhaust @14:00:34.32
Forearm.home.seek :retract @14:00:34.47 PS.reconfigure PSconfig @14:00:34.55 PS.home.seek 0ml @14:00:35.16 Hand.close @14:00:35.19
Elbow.jog 1500 @14:00:35.33 CS.reconfigure CSfast @14:00:35.36 SS.reconfigure SSconfig @14:00:35.47 CS.home.seek 0ml @14:00:35.49 SS.home.seek 0ml @14:00:35.92
Elbow.reconfigure LooseElbow @14:00:35.97 Elbow.home.seek :clear @14:00:39.40 SE.reconfigure SEconfig @14:00:39.45 SE.home.seek :down @14:00:40.21 SC.reconfigure SCsloppy @14:00:40.26 SC.home.seek :initial @14:00:43.58 PS.coast @14:00:44.29
Forearm.seek :retract @14:00:44.38 Elbow.seek :hammer @14:00:45.67 Forearm.reconfigure TightForearm @14:00:45.72 Forearm.seek 5 + 350 counts @14:00:46.78 Forearm.seek :retract @14:00:47.95 PC.reconfigure PCconfig @14:00:47.97
CC.reconfigure CCconfig @14:00:48.06 PC.home.seek :open @14:00:48.09
CC.home.seek :open @14:00:51.57 Phase [mbaPhase] @14:00:51.58 GoodNews.email "At 14:00:51 began in tube 3: mbaPhase\n/home/esp/esp2/mission/18tundeployv3.rb:123\n{1 => 22, 2 => 11, 3 => 11}\nCan@14:00:51, 13.3C, 70% humidity, 14.3psia, 13.299V, 0.645A, 0.645A avg, 8.57W",Subject: "Started 18tundeployv3 phase mbaPhase" @14:00:51.61CEST13-Oct-18
==================> STARTED Superphase (phase #2) <=== @14:00:51.63 Starting initialPurge @14:00:51.65 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:00:51.72 Hand.open @14:00:52.49 Elbow.reconfigure TightElbow @14:00:52.54 Elbow.seek FlushPuck::Garage @14:00:54.44 Elbow.reconfigure LooseElbow @14:00:54.55 Forearm.reconfigure LooseForearm @14:00:54.60 Forearm.seek :garage @14:00:55.60 Hand.close @14:00:56.37 Forearm.seek :retract @14:00:57.38 Elbow.reconfigure TightElbow @14:00:57.46 Elbow.seek CC @14:01:00.39 Elbow.reconfigure LooseElbow @14:01:00.50 Forearm.reconfigure TightForearm @14:01:00.55 Forearm.seek CC @14:01:01.87 CC.seek :closed @14:01:06.13 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.09 @14:01:06.21 Hand.open @14:01:06.94 Forearm.reconfigure LooseForearm @14:01:06.98 Forearm.seek :retract @14:01:07.97 Flushing the Collection Valve::Manifold @14:01:08.10 CSV.dial CSR @14:01:09.53 Pulling 5ml of CSR.flush @14:01:09.54 CSR.select :flush @14:01:09.67 CS.reconfigure CSconfig @14:01:09.71 CS.seek 5ml @14:01:23.54 delay 5 seconds @14:01:28.56 CSR.select :air @14:01:28.73 CS.reconfigure CSfast @14:01:28.78 CS.seek 7.501ml @14:01:35.62 CSV.dial :waste @14:01:36.97 CS.reconfigure CSconfig @14:01:37.02 CS.seek 0ml @14:01:57.13 Purging external Intake @14:01:57.26 CSV.dial :flush @14:01:57.74 CS.reconfigure CSfast @14:01:57.79 CS.seek 10ml @14:02:22.26 delay 5 seconds @14:02:27.29 CSV.dial CBV @14:02:27.33
CBV.dial CSV @14:02:28.10 CTV.dial :intake @14:02:28.89 Intake.select :open @14:02:29.02 CS.seek 0ml @14:02:54.56 Intake.select :close @14:02:54.68 CTV.dial :air @14:02:55.47 Flushing upper manifold @14:02:55.60 CSV.dial :flush @14:02:56.39 CS.seek 5ml @14:03:09.51 delay 5 seconds @14:03:14.53 CSV.dial :air @14:03:15.09 CS.seek 7ml @14:03:20.85 CSV.dial CBV @14:03:20.87
CBV.dial CSV @14:03:21.98 CTV.dial :air @14:03:22.00 CS.seek 0ml @14:03:39.19 delay 7 seconds @14:03:46.22 CS.seek 3ml @14:03:54.25 CSV.dial :waste @14:03:54.74 CS.seek 0ml @14:04:02.91 CSV.dial :flush @14:04:03.38 CS.seek 3ml @14:04:11.35 delay 5 seconds @14:04:16.37 CSV.dial :air @14:04:16.94 CS.seek 5ml @14:04:22.65 CSV.dial :air @14:04:22.68 CS.seek 0ml @14:04:35.23 delay 7 seconds @14:04:42.26 CS.seek 3ml @14:04:50.37 CSV.dial :waste @14:04:51.16 CS.seek 0ml @14:04:59.20 Garaging FlushPuck in Collection Clamp @14:04:59.28 Elbow.reconfigure TightElbow @14:04:59.33 Elbow.seek CC @14:04:59.63 Elbow.reconfigure LooseElbow @14:04:59.74 Forearm.seek CC @14:05:00.70 Hand.close @14:05:01.37 CC.seek :open @14:05:05.72 Forearm.seek :retract @14:05:06.70 Elbow.reconfigure TightElbow @14:05:06.75 Elbow.seek :garage @14:05:09.73 Elbow.reconfigure LooseElbow @14:05:09.83 Forearm.reconfigure TightForearm @14:05:09.88 Forearm.seek :garage @14:05:11.01 Hand.open @14:05:11.69 Forearm.reconfigure LooseForearm @14:05:11.73 Forearm.seek :retract @14:05:12.86 Starting mfb @14:05:12.87 pcrBAC sampling at most 150ml, wcr at most 1500ml @14:05:12.90 BACfans.email "Started mfb in tube 3 at 14:05:12CEST13-Oct-18\n{1 => 22, 2 => 11, 3 => 11}\nCan@14:00:51, 13.3C, 70% humidity, 14.3psia, 13.299V, 0.645A, 0.645A avg, 8.57W",Subject: "pcrBAC sampling at most 150ml, wcr at most 1500ml" @14:05:12.97
Loading sh1 puck from tube 3 into Collection Clamp @14:05:13.13 Readying the MFB @14:05:13.19 Homing MFB Syringe @14:05:13.21 AFV.select :holdingCoil @14:05:13.44 ATV.dial :air2 @14:05:13.48
SC.reconfigure SCtight @14:05:13.57 SC.seek 3 @14:05:14.03 AS.reconfigure ASconfig @14:05:14.08 AS.home.seek :home @14:05:15.63
SC.reconfigure SCloose @14:05:15.86 Elbow.reconfigure TightElbow @14:05:15.91 Elbow.seek 3 @14:05:17.98 Forearm.reconfigure TightForearm @14:05:18.03 Forearm.seek 3 + 700 counts @14:05:19.55 SE.reconfigure SEpick @14:05:19.74 AFV.select :carrierSolution @14:05:19.86
SE.seek beamBreak @14:05:19.98 delay 2 seconds @14:05:22.06
SE.configure SEconfig @14:05:22.12 AS.seek 1ml @14:05:22.42
Elbow.seek 3 @14:05:22.65 Forearm.seek 3 + 300 counts @14:05:22.88 Forearm.reconfigure LooseForearm @14:05:22.94 Hand.close @14:05:23.64 SE.seek :down @14:05:23.89
Forearm.seek :retract @14:05:24.82 Elbow.seek CC @14:05:25.58 SE.coast @14:05:26.51
Elbow.reconfigure LooseElbow @14:05:26.62 Forearm.reconfigure TightForearm @14:05:26.67 Forearm.seek CC @14:05:27.96 CC.seek :closed @14:05:32.28 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.06 @14:05:32.29 Hand.open @14:05:32.94 Forearm.reconfigure LooseForearm @14:05:32.99 Forearm.seek :retract @14:05:33.96 SC.coast @14:05:34.00 collectSample 150ml w/bubble point of 55psi @14:05:34.01 Priming 7ml @14:05:34.02 Intake.select :close @14:05:34.03 Exhaust.select :close @14:05:34.05 SS.hold @14:05:34.27 Calibrating pressure and temperature sensors @14:05:34.29 CTV.dial :air @14:05:34.30 SSV.dial :spit,avoiding: :exhaust @14:05:34.32 SS.reconfigure SSfast @14:05:34.38 SS.seek 5ml @14:05:37.07 SSV.dial CBV @14:05:37.11
CBV.dial SSV @14:05:38.52 delay 3.8 seconds @14:05:42.46 SSV.dial :spit @14:05:43.86 SS.seek 0ml @14:05:46.68 SSV.dial CBV @14:05:48.04 CTV.dial :intake @14:05:48.84 SSV.dial CBV @14:05:48.91
CBV.dial SSV @14:05:48.92 Allowing 40s for 7.0ml of remaining 7.0ml @14:05:48.93 Intake.select :open @14:05:51.11 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:05:51.13 SS.reconfigure SSinhale.with maxSpeed: 150 @14:05:51.18 SS.seek 7ml @14:05:54.74 Vacuum too high -- waiting up to 40s @14:05:56.35 Intake @32.8psia, vacuum from 20.0 to 24.0psi @14:05:56.37 SS.reconfigure SSinhale.with maxSpeed: 135 @14:05:56.41 SS.seek 7ml @14:05:59.14 Vacuum too high -- waiting up to 40s @14:06:00.78 Intake @32.8psia, vacuum from 20.0 to 24.0psi @14:06:00.80 SS.reconfigure SSinhale.with maxSpeed: 122 @14:06:00.85 SS.seek 7ml @14:06:02.46 Stroke completed -- waiting for pressure to equalize ... @14:06:04.42 Intake.select :close @14:06:04.57 SSV.dial :exhaust @14:06:05.38 Exhaust.select :open @14:06:05.49 SS.reconfigure SSexhale @14:06:05.54 SS.seek 0ml @14:06:09.15 Exhaust.select :close @14:06:09.27 Sampling 150.0ml @14:06:09.28 Intake.select :close @14:06:09.29 Exhaust.select :close @14:06:09.31 SS.reconfigure SSconfig @14:06:09.35 SS.hold @14:06:09.57 CTV.dial :intake @14:06:09.59 SSV.dial CBV @14:06:09.62
CBV.dial SSV @14:06:10.45 Allowing 130s for 25.0ml of remaining 150.0ml @14:06:10.46 Intake.select :open @14:06:12.63 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:06:12.65 SS.reconfigure SSinhale.with maxSpeed: 122 @14:06:12.70 SS.seek 25ml @14:06:15.18 delay 1 second @14:06:16.19 AFV.select :holdingCoil @14:06:16.30 delay 1 second @14:06:16.67
Vacuum too high -- waiting up to 40s @14:06:17.32 ATV.dial :waste @14:06:18.04
Intake @32.6psia, vacuum from 20.0 to 24.0psi @14:06:18.06 SS.reconfigure SSinhale.with maxSpeed: 110 @14:06:18.11 SS.seek 25ml @14:06:18.43 AS.reconfigure ASnorm @14:06:18.48 AS.home.seek :empty @14:06:20.55
Vacuum too high -- waiting up to 40s @14:06:22.15 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:06:22.17 SS.reconfigure SSinhale.with maxSpeed: 99 @14:06:22.22 SS.seek 25ml @14:06:24.77 Vacuum too high -- waiting up to 40s @14:06:26.11 Intake @32.9psia, vacuum from 20.0 to 24.0psi @14:06:26.13 SS.reconfigure SSinhale.with maxSpeed: 89 @14:06:26.18 SS.seek 25ml @14:06:28.83 Vacuum too high -- waiting up to 40s @14:06:30.19 Intake @32.8psia, vacuum from 20.0 to 24.0psi @14:06:30.21 SS.reconfigure SSinhale.with maxSpeed: 80 @14:06:30.26 SS.seek 25ml @14:06:33.31 Vacuum too high -- waiting up to 40s @14:06:34.67 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:06:34.69 SS.reconfigure SSinhale.with maxSpeed: 72 @14:06:34.74 SS.seek 25ml @14:06:38.33 Vacuum too high -- waiting up to 40s @14:06:39.46 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:06:39.47 SS.reconfigure SSinhale.with maxSpeed: 65 @14:06:39.52 SS.seek 25ml @14:06:43.45 Vacuum too high -- waiting up to 40s @14:06:44.54 Intake @32.8psia, vacuum from 20.0 to 24.0psi @14:06:44.56 SS.reconfigure SSinhale.with maxSpeed: 59 @14:06:44.61 SS.seek 25ml @14:06:49.80 Vacuum too high -- waiting up to 40s @14:06:50.88 Intake @32.9psia, vacuum from 20.0 to 24.0psi @14:06:50.90 SS.reconfigure SSinhale.with maxSpeed: 53 @14:06:50.95 SS.seek 25ml @14:06:52.81 Stroke completed -- waiting for pressure to equalize ... @14:06:55.39 Intake.select :close @14:06:55.54 SSV.dial :exhaust @14:06:56.34 Exhaust.select :open @14:06:56.46 SS.reconfigure SSexhale @14:06:56.51 SS.seek 0ml @14:07:05.26 Exhaust.select :close @14:07:05.38 SSV.dial CBV @14:07:05.41
CBV.dial SSV @14:07:06.24 Allowing 130s for 25.0ml of remaining 125.0ml @14:07:06.25 Intake.select :open @14:07:08.42 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:07:08.44 SS.reconfigure SSinhale.with maxSpeed: 53 @14:07:08.49 SS.seek 25ml @14:07:11.04 ATV.dial :waste @14:07:11.06 ARV.dial between carrier and air1 @14:07:11.15 ASV.dial :waste1 @14:07:18.58
Vacuum too high -- waiting up to 40s @14:07:19.40 Intake @32.7psia, vacuum from 20.0 to 24.0psi @14:07:19.42 SS.reconfigure SSinhale.with maxSpeed: 48 @14:07:19.47 SS.seek 25ml @14:07:46.46 Stroke completed -- waiting for pressure to equalize ... @14:07:49.66 Intake.select :close @14:07:49.81 SSV.dial :exhaust @14:07:50.62 Exhaust.select :open @14:07:51.26 SS.reconfigure SSexhale @14:07:51.32 SS.seek 0ml @14:08:00.17 Exhaust.select :close @14:08:00.29 SSV.dial CBV @14:08:00.31
CBV.dial SSV @14:08:01.13 Allowing 130s for 25.0ml of remaining 100.0ml @14:08:01.14 Intake.select :open @14:08:03.32 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:08:03.33 SS.reconfigure SSinhale.with maxSpeed: 48 @14:08:03.38 SS.seek 25ml @14:08:36.62 Vacuum too high -- waiting up to 40s @14:08:37.45 Intake @32.7psia, vacuum from 20.0 to 24.0psi @14:08:37.47 SS.reconfigure SSinhale.with maxSpeed: 43 @14:08:37.52 SS.seek 25ml @14:08:42.51 Stroke completed -- waiting for pressure to equalize ... @14:08:45.08 Intake.select :close @14:08:45.23 SSV.dial :exhaust @14:08:46.04 Exhaust.select :open @14:08:46.16 SS.reconfigure SSexhale @14:08:46.21 SS.seek 0ml @14:08:55.15 Exhaust.select :close @14:08:55.27 SSV.dial CBV @14:08:55.30
CBV.dial SSV @14:08:56.13 Allowing 130s for 25.0ml of remaining 75.0ml @14:08:56.14 Intake.select :open @14:08:58.31 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:08:58.33 SS.reconfigure SSinhale.with maxSpeed: 43 @14:08:58.38 SS.seek 25ml @14:09:40.14 Stroke completed -- waiting for pressure to equalize ... @14:09:42.70 Intake.select :close @14:09:42.85 SSV.dial :exhaust @14:09:43.67 Exhaust.select :open @14:09:43.79 SS.reconfigure SSexhale @14:09:43.84 SS.seek 0ml @14:09:52.53 Exhaust.select :close @14:09:52.69 SSV.dial CBV @14:09:52.72
CBV.dial SSV @14:09:53.54 Allowing 130s for 25.0ml of remaining 50.0ml @14:09:53.55 Intake.select :open @14:09:55.73 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:09:55.74 SS.reconfigure SSinhale.with maxSpeed: 43 @14:09:55.79 SS.seek 25ml @14:10:37.59 Stroke completed -- waiting for pressure to equalize ... @14:10:40.17 Intake.select :close @14:10:40.32 SSV.dial :exhaust @14:10:41.12 Exhaust.select :open @14:10:41.24 SS.reconfigure SSexhale @14:10:41.29 SS.seek 0ml @14:10:50.31 Exhaust.select :close @14:10:50.43 SSV.dial CBV @14:10:50.46
CBV.dial SSV @14:10:51.30 Allowing 130s for 25.0ml of remaining 25.0ml @14:10:51.31 Intake.select :open @14:10:53.48 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:10:53.50 SS.reconfigure SSinhale.with maxSpeed: 43 @14:10:53.55 SS.seek 25ml @14:11:34.81 Stroke completed -- waiting for pressure to equalize ... @14:11:37.38 Intake.select :close @14:11:37.53 SSV.dial :exhaust @14:11:38.36 Exhaust.select :open @14:11:38.48 SS.reconfigure SSexhale @14:11:38.53 SS.seek 0ml @14:11:47.87 Exhaust.select :close @14:11:47.99 Sampled 150.0ml @14:11:48.01 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @14:11:48.02 Intake.select :close @14:11:48.08 CSV.dial :waste @14:11:48.15 CS.seek 0ml @14:11:48.26 CSV.dial :air @14:11:48.29 SSV.dial CBV,avoiding: :exhaust @14:11:48.34
CBV.dial SSV @14:11:49.27 CS.seek 10ml @14:11:50.33
CTV.dial :air @14:11:51.12 delay 15 seconds @14:12:06.15 SSV.dial :spit,avoiding: :exhaust @14:12:07.54 CTV.dial CSV,avoiding: :intake @14:12:07.63
SS.seek 0ml @14:12:07.71 SSV.dial CBV,avoiding: :exhaust @14:12:13.82 CBV.dial between SSV and waste2 @14:12:14.24 CTV.dial CSV,avoiding: :air @14:12:14.27
CSV.dial CTV @14:12:14.79 CS.reconfigure CSpressurize @14:12:14.83 CS.seek 0ml @14:12:41.03 BubblePoint: Intake @69.4psia, Outlet @14.5psia @14:12:41.04 CBV.dial SSV @14:12:41.41 SS.reconfigure SSconfig @14:12:41.46 SS.seek 10ml @14:12:47.16 delay 15 seconds @14:13:02.30 Evacuated: Intake @35.9psia, Outlet @0.9psia @14:13:02.32 SSV.dial :spit,avoiding: :exhaust @14:13:03.71 SS.reconfigure SSexhale @14:13:03.76 SS.seek 0ml @14:13:08.97 SS.reconfigure SSfast @14:13:09.02 SS.seek 5ml @14:13:11.61 SSV.dial CBV,avoiding: :exhaust @14:13:11.64
CBV.dial SSV @14:13:13.05 CTV.dial :air,avoiding: :intake @14:13:14.44 CSV.dial CTV @14:13:14.49
SS.seek 10ml @14:13:15.48 CSV.dial :air @14:13:15.98 CS.reconfigure CSfast @14:13:16.03 CS.seek 0ml @14:13:16.32 CS.coast @14:13:16.97
SSV.dial :spit,avoiding: :exhaust @14:13:18.35 SS.reconfigure SSexhale @14:13:18.40 SS.seek 0ml @14:13:23.72 SS.reconfigure SSfast @14:13:23.77 SS.seek 5ml @14:13:26.36 SSV.dial CBV,avoiding: :exhaust @14:13:26.39
CBV.dial SSV @14:13:28.83 SSV.dial :spit,avoiding: :exhaust @14:13:30.22 SS.reconfigure SSconfig @14:13:30.27 SS.seek 0ml @14:13:33.43 SS.coast @14:13:33.48 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @14:13:33.49 Intake.select :close @14:13:33.57 CSV.dial :waste @14:13:33.69 SSV.dial CBV,avoiding: :exhaust @14:13:33.72
CBV.dial SSV @14:13:34.39 CS.seek 0ml @14:13:34.47 CSV.dial :air @14:13:35.11
CTV.dial :air @14:13:35.12 delay 15 seconds @14:13:35.25 CS.seek 10ml @14:13:50.15
SSV.dial :spit,avoiding: :exhaust @14:13:52.05 CTV.dial CSV,avoiding: :intake @14:13:52.09
SS.reconfigure SSexhale @14:13:52.20 SS.seek 0ml @14:13:52.26 SSV.dial CBV,avoiding: :exhaust @14:13:59.51 CBV.dial between SSV and waste2 @14:13:59.94 CSV.dial between air and CTV @14:14:00.28 CS.seek 8.275ml @14:14:05.30 CTV.dial CSV,avoiding: :air @14:14:05.33
CSV.dial CTV @14:14:05.66 CS.reconfigure CSpressurize @14:14:05.72 CS.seek 0ml @14:14:27.51 CSV.dial :air @14:14:28.00 CS.reconfigure CSfast @14:14:28.05 CS.seek 10ml @14:14:52.17 CSV.dial between air and CTV @14:14:52.53 CS.seek 3.127ml @14:15:09.39 CTV.dial CSV,avoiding: :air @14:15:09.42
CSV.dial CTV @14:15:09.86 CS.reconfigure CSpressurize @14:15:09.91 CS.seek 0ml @14:15:18.11 BubblePoint: Intake @69.4psia, Outlet @14.1psia @14:15:18.12 CBV.dial SSV @14:15:18.47 SS.reconfigure SSconfig @14:15:18.52 SS.seek 10ml @14:15:23.91 delay 15 seconds @14:15:39.05 Evacuated: Intake @40.3psia, Outlet @15.2psia @14:15:39.06 SSV.dial :spit,avoiding: :exhaust @14:15:40.46 SS.reconfigure SSexhale @14:15:40.51 SS.seek 0ml @14:15:45.52 SS.reconfigure SSfast @14:15:45.57 SS.seek 5ml @14:15:48.61 SSV.dial CBV,avoiding: :exhaust @14:15:48.64
CBV.dial SSV @14:15:50.05 CTV.dial :air,avoiding: :intake @14:15:51.44 CSV.dial CTV @14:15:51.48
SS.seek 10ml @14:15:52.47 CSV.dial :air @14:15:52.94 CS.reconfigure CSfast @14:15:52.99 CS.seek 0ml @14:15:54.10
SSV.dial :spit,avoiding: :exhaust @14:15:54.32 CS.coast @14:15:55.52
SS.reconfigure SSexhale @14:15:55.57 SS.seek 0ml @14:16:00.59 SS.reconfigure SSfast @14:16:00.66 SS.seek 5ml @14:16:03.38 SSV.dial CBV,avoiding: :exhaust @14:16:03.41
CBV.dial SSV @14:16:05.84 SSV.dial :spit,avoiding: :exhaust @14:16:07.24 SS.reconfigure SSconfig @14:16:07.29 SS.seek 0ml @14:16:10.45 SS.coast @14:16:10.49 Lysing: Adding 1.5ml lysis @14:16:10.51 CBV.dial between CSV and waste1,avoiding: :DBV @14:16:12.08 CTV.dial CSV @14:16:13.47 CSV.dial CSR @14:16:14.31 Pulling 1ml of CSR.lysis @14:16:14.32 CSR.select :lysis @14:16:14.45 CS.reconfigure CSconfig @14:16:14.49 CS.seek 1ml @14:16:18.14 delay 5 seconds @14:16:23.16 CSR.select :air @14:16:23.35 CS.seek 6ml @14:16:37.08 delay 2 seconds @14:16:39.09 CSV.dial :waste1 @14:16:40.47 CS.reconfigure CSfast @14:16:40.52 CS.seek :empty @14:16:55.32 CSV.dial CSR @14:16:56.74 Pulling 1.5ml of CSR.lysis @14:16:56.75 CSR.select :lysis @14:16:56.87 CS.reconfigure CSconfig @14:16:56.91 CS.seek 1.5ml @14:17:01.86 delay 5 seconds @14:17:06.88 CSR.select :air @14:17:07.05 CS.seek 4.501ml @14:17:15.86 delay 2 seconds @14:17:17.87 CSV.dial CTV @14:17:18.36 CS.seek 1.275ml @14:17:27.58 Heating sh1 puck from tube 3 in Collection Clamp to 85C for 5:00 @14:17:27.60 CH.seek 85C,8:00 @14:20:38.22 CH.hold 5:00,85C @14:25:38.40 Collection Puck Heater at 85C after lysing @14:25:38.42 CS.seek 0ml @14:25:42.71 CTV.dial between spare and air,avoiding: :intake @14:25:43.82 CSV.dial :air @14:25:44.84 CS.seek 5ml @14:25:58.55 CSV.dial CTV @14:25:59.04 delay 2 seconds @14:26:01.06 CSV.dial :air @14:26:01.54 delay 2 seconds @14:26:03.55 CSV.dial CTV @14:26:04.04 delay 2 seconds @14:26:06.06 CSV.dial :air @14:26:06.54 delay 2 seconds @14:26:08.56 CS.seek 0ml @14:26:22.28 Recovering Lysate into Collection syringe @14:26:22.29 CSV.dial CBV @14:26:23.36 CBV.dial CSV @14:26:23.77 CS.seek 5ml @14:26:37.59 delay 5 seconds @14:26:42.61 CTV.dial :air @14:26:43.18 CS.seek 7ml @14:26:49.34 delay 5 seconds @14:26:54.35 CSV.dial :air2 @14:26:54.83 CS.seek 10ml @14:27:03.50 CSV.dial between air and CTV,avoiding: CBV @14:27:04.70 Lysing Complete @14:27:04.78 Storing sh1 puck in Collection Clamp to tube 2 @14:27:04.97 SC.reconfigure SCtight @14:27:05.02 SC.seek 2 @14:27:07.15 SC.reconfigure SCloose @14:27:07.38 Elbow.reconfigure TightElbow @14:27:07.43 Elbow.seek 2 @14:27:09.33 Forearm.reconfigure TightForearm @14:27:09.38 Forearm.seek 2 + 700 counts @14:27:10.49 SE.reconfigure SEpick @14:27:10.56 SE.seek beamBreak @14:27:12.67 SE.configure SEconfig @14:27:12.85 Elbow.reconfigure LimpElbow @14:27:12.90 Forearm.reconfigure LooseForearm @14:27:12.95 Forearm.seek 2 @14:27:13.21 Elbow.hold @14:27:13.28 Elbow.reconfigure TightElbow @14:27:13.39 SE.seek 10.08 Pucks below Top Plate @14:27:13.85 Forearm.force -20 @14:27:13.89 Forearm.seek 2 + 349 counts @14:27:14.23 Forearm.reconfigure TightForearm @14:27:14.28 Forearm.seek 2 + 700 counts @14:27:14.51 SE.seek 11.08 Pucks below Top Plate @14:27:15.28 Forearm.reconfigure LooseForearm @14:27:15.33 Forearm.seek :retract @14:27:16.23 Elbow.seek CC @14:27:17.95 Elbow.reconfigure LooseElbow @14:27:18.06 Forearm.seek CC @14:27:19.00 Hand.close @14:27:19.66 CC.seek :open @14:27:24.71 Forearm.seek :retract @14:27:25.71 Elbow.reconfigure TightElbow @14:27:25.76 Elbow.seek 2 @14:27:27.64 Elbow.reconfigure LimpElbow @14:27:27.69 Forearm.seek 2 @14:27:28.73 Elbow.hold @14:27:28.80 Elbow.reconfigure TightElbow @14:27:28.85 Hand.open @14:27:29.69 Forearm.force -20 @14:27:29.73 Forearm.seek 2 + 340 counts @14:27:30.06 Forearm.reconfigure TightForearm @14:27:30.11 Forearm.seek 2 + 700 counts @14:27:30.48 SE.seek :down @14:27:31.64
Forearm.reconfigure LooseForearm @14:27:31.69 Forearm.seek :retract @14:27:32.24 SE.coast @14:27:32.60
SC.coast @14:27:32.64 Tranferring lysate to PRV @14:27:32.65 CSV.dial :air @14:27:32.98 CS.seek 10ml @14:27:33.05 CSV.dial between air and CTV @14:27:33.39 AIV.dial :bypass @14:27:33.48 Beginning samplePassC @14:27:33.49 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:27:33.57 Hand.open @14:27:33.91 Elbow.seek FlushPuck::Garage @14:27:35.93 Elbow.reconfigure LooseElbow @14:27:36.04 Forearm.seek :garage @14:27:37.03 Hand.close @14:27:37.78 Forearm.seek :retract @14:27:38.81 Elbow.reconfigure TightElbow @14:27:38.86 Elbow.seek CC @14:27:41.76 Elbow.reconfigure LooseElbow @14:27:41.87 Forearm.reconfigure TightForearm @14:27:41.92 Forearm.seek CC @14:27:43.22 CC.seek :closed @14:27:47.50 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.08 @14:27:47.57 Hand.open @14:27:48.21 Forearm.reconfigure LooseForearm @14:27:48.26 Forearm.seek :retract @14:27:49.77 AWV.select :air @14:27:49.79 AIV.dial :bypass @14:27:49.80 PSV.dial PSR @14:27:51.34 Pulling 1.5ml of PSR.flush @14:27:51.35 PSR.select :flush @14:27:51.48 PS.seek 1.5ml @14:28:07.57 delay 5 seconds @14:28:12.60 PSV.dial :waste1 @14:28:13.06 PS.seek 0ml @14:28:29.06 PSV.dial PSR @14:28:29.55 PSR.select :air @14:28:29.68 PS.seek 2.5ml @14:28:55.79 PRV.dial CBV @14:28:55.82
CBV.dial PRV @14:28:56.68 PSV.dial :mixloop @14:28:57.75 CTV.dial :air,avoiding: :intake @14:28:57.76 PTV.dial :PRVmixing @14:28:57.84 PS.seek 0ml @14:29:23.94 PSV.dial :air @14:29:24.42 PS.seek 2.5ml @14:29:50.57 PSV.dial :mixloop @14:29:51.07 PS.seek 0ml @14:30:17.22 PSV.dial :air @14:30:17.70 PS.seek 2.5ml @14:30:43.82 PSV.dial :mixloop @14:30:44.31 PS.seek 0ml @14:31:10.48 Garaging FlushPuck in Collection Clamp @14:31:10.56 Elbow.reconfigure TightElbow @14:31:10.61 Elbow.seek CC @14:31:10.86 Elbow.reconfigure LooseElbow @14:31:10.97 Forearm.seek CC @14:31:11.96 Hand.close @14:31:12.53 CC.seek :open @14:31:16.91 Forearm.seek :retract @14:31:17.91 Elbow.reconfigure TightElbow @14:31:17.96 Elbow.seek :garage @14:31:21.00 Elbow.reconfigure LooseElbow @14:31:21.11 Forearm.reconfigure TightForearm @14:31:21.16 Forearm.seek :garage @14:31:22.33 Hand.open @14:31:22.98 Forearm.reconfigure LooseForearm @14:31:23.02 Forearm.seek :retract @14:31:24.06 Connecting PS to CS to transfer sample @14:31:24.07 CBV.dial AIV @14:31:24.08 AIV.dial :bypass @14:31:24.09 PSV.dial :mixloop @14:31:24.10 PTV.dial :PRVmixing @14:31:24.12 PRV.dial AIV @14:31:24.13 CTV.dial :air,avoiding: :intake @14:31:24.15 Loading lyfil puck from tube 3 into Collection Clamp @14:31:24.41 SC.reconfigure SCtight @14:31:24.46 SC.seek 3 @14:31:26.57 SC.reconfigure SCloose @14:31:26.80 Elbow.reconfigure TightElbow @14:31:26.85 Elbow.seek 3 @14:31:28.91 Forearm.reconfigure TightForearm @14:31:28.96 Forearm.seek 3 + 700 counts @14:31:29.97 SE.reconfigure SEpick @14:31:30.05 SE.seek beamBreak @14:31:32.30 SE.configure SEconfig @14:31:32.98 Elbow.seek 3 @14:31:33.18 Forearm.seek 3 + 300 counts @14:31:33.42 Forearm.reconfigure LooseForearm @14:31:33.46 Hand.close @14:31:34.05 SE.seek :down @14:31:34.28
Forearm.seek :retract @14:31:35.25 Elbow.seek CC @14:31:36.07 SE.coast @14:31:36.90
Elbow.reconfigure LooseElbow @14:31:37.01 Forearm.reconfigure TightForearm @14:31:37.06 Forearm.seek CC @14:31:38.27 CC.seek :closed @14:31:42.51 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.14 @14:31:42.52 Hand.open @14:31:43.22 Forearm.reconfigure LooseForearm @14:31:43.28 Forearm.seek :retract @14:31:44.27 SC.coast @14:31:44.31 CTV.dial CSV,avoiding: :intake @14:31:45.72 CSV.dial CTV @14:31:46.04 CS.seek 5ml @14:31:59.88 delay 1:20 @14:33:19.91 CS.seek 2.5ml @14:33:27.38 delay 40 seconds @14:34:07.41 CS.seek 0ml @14:34:14.82 delay 1:00 @14:35:14.84 PSV.dial :air @14:35:15.33 PS.seek 2.5ml @14:35:41.44 PSV.dial :mixloop @14:35:41.92 delay 3 seconds @14:35:44.94 PSV.dial :air @14:35:45.42 delay 2 seconds @14:35:47.43 PSV.dial :mixloop @14:35:47.91 delay 3 seconds @14:35:50.92 PSV.dial :waste1 @14:35:52.42 PS.seek :empty @14:36:18.57 CBV.dial :waste1 @14:36:19.07 delay 5 seconds @14:36:24.09 CBV.dial AIV @14:36:24.58 delay 10 seconds @14:36:34.60 CBV.dial :waste1 @14:36:35.13 delay 10 seconds @14:36:45.15 CBV.dial AIV @14:36:45.65 delay 10 seconds @14:36:55.68 CTV.dial CSV,avoiding: :intake @14:36:55.69 CBV.dial between AIV and waste1 @14:36:56.05 CSV.dial :air @14:36:56.65 CS.seek 10ml @14:37:23.12 CSV.dial CTV @14:37:23.58 CS.seek 0ml @14:37:50.18 CBV.dial :waste1 @14:37:50.52 delay 10 seconds @14:38:00.55 CTV.dial :air,avoiding: :intake @14:38:01.99 Storing lyfil puck in Collection Clamp to tube 2 @14:38:02.25 SC.reconfigure SCtight @14:38:02.30 SC.seek 2 @14:38:04.44 SC.reconfigure SCloose @14:38:05.10 Elbow.reconfigure TightElbow @14:38:05.23 Elbow.seek 2 @14:38:07.14 Forearm.reconfigure TightForearm @14:38:07.19 Forearm.seek 2 + 700 counts @14:38:08.21 SE.reconfigure SEpick @14:38:08.28 SE.seek beamBreak @14:38:10.34 SE.configure SEconfig @14:38:10.51 Elbow.reconfigure LimpElbow @14:38:10.56 Forearm.reconfigure LooseForearm @14:38:10.62 Forearm.seek 2 @14:38:10.95 Elbow.hold @14:38:11.02 Elbow.reconfigure TightElbow @14:38:11.13 SE.seek 11.08 Pucks below Top Plate @14:38:11.60 Forearm.force -20 @14:38:11.64 Forearm.seek 2 + 339 counts @14:38:11.95 Forearm.reconfigure TightForearm @14:38:12.00 Forearm.seek 2 + 700 counts @14:38:12.24 SE.seek 12.08 Pucks below Top Plate @14:38:12.89 Forearm.reconfigure LooseForearm @14:38:12.94 Forearm.seek :retract @14:38:13.92 Elbow.seek CC @14:38:15.68 Elbow.reconfigure LooseElbow @14:38:15.79 Forearm.seek CC @14:38:16.74 Hand.close @14:38:17.43 CC.seek :open @14:38:21.91 Forearm.seek :retract @14:38:22.88 Elbow.reconfigure TightElbow @14:38:22.93 Elbow.seek 2 @14:38:24.74 Elbow.reconfigure LimpElbow @14:38:24.80 Forearm.seek 2 @14:38:25.81 Elbow.hold @14:38:25.88 Elbow.reconfigure TightElbow @14:38:25.93 Hand.open @14:38:26.67 Forearm.force -20 @14:38:26.71 Forearm.seek 2 + 342 counts @14:38:27.02 Forearm.reconfigure TightForearm @14:38:27.07 Forearm.seek 2 + 700 counts @14:38:27.37 SE.seek :down @14:38:28.51
Forearm.reconfigure LooseForearm @14:38:28.56 Forearm.seek :retract @14:38:29.04 SE.coast @14:38:29.46
SC.coast @14:38:29.49 PSV.dial :air @14:38:30.64 PS.seek 0.499ml @14:38:36.57 delay 3 seconds @14:38:39.59 PSV.dial :mixloop @14:38:40.06 PRV.dial :air @14:38:40.55 delay 3 seconds @14:38:43.56 PRV.dial CBV @14:38:44.04 CBV.dial AIV @14:38:44.53 PRV.dial between CBV and air @14:38:45.32 PRV.dial :air @14:38:45.79 PS.reconfigure PSslow @14:38:45.84 PS.seek 0.599ml @14:38:48.35 PRV.dial :waste2 @14:38:49.13 PS.reconfigure PSconfig @14:38:49.18 PS.seek :empty @14:38:56.08 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:38:56.15 Hand.open @14:38:56.55 Elbow.seek FlushPuck::Garage @14:38:58.51 Elbow.reconfigure LooseElbow @14:38:58.62 Forearm.seek :garage @14:38:59.64 Hand.close @14:39:00.49 Forearm.seek :retract @14:39:01.54 Elbow.reconfigure TightElbow @14:39:01.59 Elbow.seek CC @14:39:04.43 Elbow.reconfigure LooseElbow @14:39:04.53 Forearm.reconfigure TightForearm @14:39:04.58 Forearm.seek CC @14:39:05.89 CC.seek :closed @14:39:10.15 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.05 @14:39:10.23 Hand.open @14:39:10.99 Forearm.reconfigure LooseForearm @14:39:11.04 Forearm.seek :retract @14:39:12.10 Clearing PML for modified lysate @14:39:12.12 CTV.dial :air,avoiding: :intake @14:39:12.13 PRV.dial :waste2 @14:39:12.14 PTV.dial :PRV,avoiding: :puck @14:39:12.94 delay 2 seconds @14:39:14.96 PTV.dial :PRVmixing,avoiding: :puck @14:39:15.74 delay 2 seconds @14:39:17.76 PSV.dial :PML @14:39:17.77 delay 2 seconds @14:39:19.79 PRV.dial :air @14:39:20.58 delay 2 seconds @14:39:22.66 PS.seek 1.499ml @14:39:38.68 delay 5 seconds @14:39:43.70 PS.seek 2.5ml @14:39:54.67 PSV.dial :air @14:39:55.14 PS.seek 0ml @14:40:21.36 mixing lysate with speDil @14:40:21.37 PSV.dial :PML @14:40:21.83 CBV.dial :PRV @14:40:21.84 PRV.dial :air @14:40:21.85 delay 2 seconds @14:40:23.87 PRV.dial :speDil @14:40:24.36 delay 2 seconds @14:40:26.45 PS.reconfigure PSslow1 @14:40:26.50 PS.seek 0.225ml @14:40:50.24 delay 5 seconds @14:40:55.26 PRV.dial CBV @14:40:56.07 delay 2 seconds @14:40:58.15 PS.seek 0.475ml @14:41:24.40 delay 5 seconds @14:41:29.42 PRV.dial :air @14:41:29.93 delay 2 seconds @14:41:31.96 PS.seek 0.965ml @14:42:22.43 delay 10 seconds @14:42:32.45 PRV.dial CBV @14:42:32.94 CTV.dial :air,avoiding: :intake @14:42:32.95 PSV.dial :air @14:42:33.41 PS.reconfigure PSconfig @14:42:33.46 PS.seek 2.5ml @14:42:49.83 delay 3 seconds @14:42:52.84 PSV.dial PTV @14:42:53.63 PTV.dial :PRV,avoiding: :puck @14:42:54.41 PS.seek 0ml @14:43:20.51 PSV.dial :air @14:43:21.29 PS.seek 2.5ml @14:43:47.43 delay 3 seconds @14:43:50.44 PSV.dial PTV @14:43:51.23 PTV.dial :PRV,avoiding: :puck @14:43:51.25 PS.seek 0ml @14:44:17.40 Pushing modified lysate into AIV Injection Loop @14:44:17.41 CBV.dial AIV @14:44:17.42 AIV.dial :bypass @14:44:17.43 PRV.dial CBV @14:44:17.45 PSV.dial :air @14:44:18.23 PS.seek 2.5ml @14:44:44.37 delay 3 seconds @14:44:47.38 PSV.dial :PML @14:44:47.87 PTV.dial :PRVmixing,avoiding: :puck @14:44:48.72 PS.reconfigure PSslow1 @14:44:48.77 PS.seek 1.74ml @14:46:06.09 delay 5 seconds @14:46:11.11 AIV.dial :sample @14:46:11.71 AWV.select :waste @14:46:11.89 PS.reconfigure PSslow2 @14:46:11.94 PS.seek 1.22ml @14:47:59.59 delay 15 seconds @14:48:14.61 AIV.dial :bypass @14:48:15.20 PS.reconfigure PSconfig @14:48:15.25 PS.seek 0ml @14:48:28.45 PSV.dial :air @14:48:28.93 PS.seek 2.5ml @14:48:55.11 PSV.dial :PML @14:48:55.59 PS.seek 0ml @14:49:21.70 AIV.dial :coilToMFB @14:49:21.72 AWV.select :air @14:49:21.88 Extracting Nucleic Acid from AIV @14:49:21.92
Beginning samplePassD @14:49:21.98 SPE.hold 2:46:40,55C @14:49:22.04 ASV.dial :SPEdelivery @14:49:22.12
AWV.select :air @14:49:22.13 AIV.dial :bypass @14:49:22.14 PSV.dial PSR @14:49:22.44 delay 20 seconds @14:49:23.32
Pulling 1.5ml of PSR.killMFB @14:49:23.33 PSR.select :killMFB @14:49:23.46 PS.seek 1.5ml @14:49:39.53 delay 5 seconds @14:49:42.47 primeLysateINT @14:49:42.48 AWV.select :air @14:49:42.49 AIV.dial :coilToMFB @14:49:42.50 ATV.dial AIV @14:49:44.22 AS.reconfigure ASslow1 @14:49:44.27 AS.seek 0.299ml @14:49:44.54
PSV.dial :waste1 @14:49:45.04 PS.seek 0ml @14:50:01.05 PSV.dial PSR @14:50:01.55 PSR.select :air @14:50:01.69 PS.seek 2.5ml @14:50:27.81 PRV.dial CBV @14:50:27.84
CBV.dial PRV @14:50:27.85 PSV.dial :mixloop @14:50:28.96 CTV.dial :air,avoiding: :intake @14:50:28.97 PTV.dial :PRVmixing @14:50:28.99 PS.seek 0ml @14:50:44.96 delay 10 seconds @14:50:54.98 ATV.dial :air2 @14:50:55.05
PSV.dial PSR @14:50:56.33 AS.seek 0.329ml @14:50:56.46
Pulling 1.5ml of PSR.flush @14:50:56.47 PSR.select :flush @14:50:56.60 PS.seek 1.5ml @14:51:02.62 ATV.dial :waste2 @14:51:03.19 AS.reconfigure ASnorm @14:51:03.24 AS.seek :empty @14:51:12.56
delay 5 seconds @14:51:17.59 PSV.dial :waste1 @14:51:18.10 PS.seek 0ml @14:51:20.26 Lysate primed! @14:51:20.27 AFV.select :holdingCoil @14:51:20.28 ATV.dial :air2 @14:51:20.90 AS.reconfigure ASslow1 @14:51:20.95 AS.seek 0.069ml @14:51:34.14
PSV.dial PSR @14:51:34.76 PSR.select :air @14:51:34.89 PS.seek 2.5ml @14:51:35.32 delay 3 seconds @14:51:38.33 ATV.dial :AIV @14:51:39.18 AS.seek 0.469ml @14:52:01.12 PRV.dial CBV @14:52:01.15
CBV.dial PRV @14:52:01.16 PSV.dial :mixloop @14:52:02.29 CTV.dial :air,avoiding: :intake @14:52:02.31 PTV.dial :PRVmixing @14:52:02.33 PS.seek 0ml @14:52:28.39 PSV.dial PSR @14:52:29.56 Pulling 1.5ml of PSR.flush @14:52:29.57 PSR.select :flush @14:52:29.70 PS.seek 1.5ml @14:52:45.78 delay 5 seconds @14:52:50.80 PSV.dial :waste1 @14:52:51.42 PS.seek 0ml @14:52:59.88 delay 5 seconds @14:53:04.96 Solid Phase Extraction Heater at 48.7C @14:53:04.97 ATV.dial ASV @14:53:05.37 ASV.dial :SPEdelivery @14:53:05.46 AS.seek 0.041ml @14:53:07.39
PSV.dial PSR @14:53:07.89 PSR.select :air @14:53:08.02 PS.seek 2.5ml @14:53:34.15 PRV.dial CBV @14:53:34.18
CBV.dial PRV @14:53:34.19 PSV.dial :mixloop @14:53:35.31 CTV.dial :air,avoiding: :intake @14:53:35.32 PTV.dial :PRVmixing @14:53:35.34 PS.seek 0ml @14:54:01.55 PSV.dial PSR @14:54:02.70 Pulling 1.5ml of PSR.flush @14:54:02.71 PSR.select :flush @14:54:02.84 PS.seek 1.5ml @14:54:18.87 delay 5 seconds @14:54:23.89 PSV.dial :waste1 @14:54:24.40 PS.seek 0ml @14:54:31.79 delay 10 seconds @14:54:40.39
PSV.dial PSR @14:54:40.88 PSR.select :air @14:54:41.02 PS.seek 2.5ml @14:54:41.82 rinseLysateLineINT @14:54:41.83 AFV.select :carrierSolution @14:54:41.94 delay 2 seconds @14:54:43.97 AS.reconfigure ASconfig @14:54:44.02 AS.seek 1ml @14:55:07.22 PRV.dial CBV @14:55:07.25
CBV.dial PRV @14:55:07.26 PSV.dial :mixloop @14:55:08.34 CTV.dial :air,avoiding: :intake @14:55:08.35 PTV.dial :PRVmixing @14:55:08.37 PS.reconfigure PSslow @14:55:08.42 PS.seek 1.2ml @14:55:35.25 PSV.dial :waste1 @14:55:36.64 PS.reconfigure PSconfig @14:55:36.69 PS.seek 0ml @14:55:39.44 delay 1 second @14:55:40.45 AFV.select :holdingCoil @14:55:40.57 delay 1 second @14:55:41.59 ATV.dial AIV @14:55:42.12 AIV.dial :coilToMFB @14:55:42.13 AWV.select :waste @14:55:42.24 AS.reconfigure ASslow0 @14:55:42.82 AS.seek 0ml @14:55:49.65
PSV.dial :air @14:55:50.74 PS.seek 2.5ml @14:56:16.80 PSV.dial :mixloop @14:56:17.26 PS.reconfigure PSslow @14:56:17.31 PS.seek 0ml @14:57:08.49 PSV.dial :air @14:57:08.98 PS.reconfigure PSconfig @14:57:09.03 PS.seek 2.5ml @14:57:23.70 ATV.dial :air2 @14:57:24.36 AS.reconfigure ASslow1 @14:57:24.41 AS.seek 0.05ml @14:57:34.75 ATV.dial :AIV @14:57:35.24
PSV.dial :mixloop @14:57:35.56 AS.seek 0.01ml @14:57:35.73
PS.reconfigure PSslow @14:57:35.78 PS.seek 0ml @14:57:43.88 ATV.dial :waste @14:57:45.42 AS.seek :empty @14:57:47.91 ethanol wash @14:57:47.92 ATV.dial :air2 @14:57:49.11 AS.reconfigure ASslow2 @14:57:49.17 AS.seek 0.009ml @14:57:59.71 delay 3 seconds @14:58:02.74 Pulling 0.4ml of Ethanol Wash @14:58:02.75 ATV.dial :ethanol @14:58:03.55 AS.reconfigure ASslow1 @14:58:03.60 AS.seek 0.409ml @14:58:26.94
Cleaning Collection Syringe @14:58:26.95 CSV.dial CSR @14:58:27.48 Pulling 4ml of CSR.flush @14:58:27.49 CSR.select :flush @14:58:27.62 CS.seek 4ml @14:58:38.84 delay 5 seconds @14:58:43.86 CSR.select :air @14:58:44.00 CS.seek 10ml @14:59:00.34 CSV.dial CTV @14:59:00.37
CTV.dial CSV @14:59:01.84 CBV.dial :waste1 @14:59:02.35 CS.seek 0ml @14:59:24.31 delay 3 seconds @14:59:27.33 ATV.dial :air1 @14:59:27.73 delay 3 seconds @14:59:28.86
CSV.dial CSR @14:59:29.41 Pulling 4ml of CSR.flush @14:59:29.42 CSR.select :flush @14:59:29.55 CS.seek 4ml @14:59:30.82 AS.seek 0.434ml @14:59:36.12 delay 3 seconds @14:59:39.14 ATV.dial :ethanol @14:59:39.74 AS.seek 0.419ml @14:59:40.68
delay 5 seconds @14:59:43.03 delay 3 seconds @14:59:45.70
CSR.select :air @14:59:45.83 CS.seek 10ml @14:59:46.05 ATV.dial ASV @14:59:47.33 AS.seek 0.092ml @15:00:02.21 CSV.dial CTV @15:00:02.24
CTV.dial CSV @15:00:02.73 CBV.dial :waste1 @15:00:02.75 CS.seek 0ml @15:00:29.31 Garaging FlushPuck in Collection Clamp @15:00:29.39 Elbow.reconfigure TightElbow @15:00:29.44 Elbow.seek CC @15:00:29.71 Elbow.reconfigure LooseElbow @15:00:29.82 Forearm.seek CC @15:00:30.81 Hand.close @15:00:31.45 CC.seek :open @15:00:36.27 Forearm.seek :retract @15:00:37.34 Elbow.reconfigure TightElbow @15:00:37.39 Elbow.seek :garage @15:00:40.39 Elbow.reconfigure LooseElbow @15:00:40.49 Forearm.reconfigure TightForearm @15:00:40.54 Forearm.seek :garage @15:00:41.74 Hand.open @15:00:42.39 Forearm.reconfigure LooseForearm @15:00:42.44 Forearm.seek :retract @15:00:43.43 Whole Cell Archive sampling 1500ml @15:00:43.45 Loading wcr puck from tube 3 into Collection Clamp @15:00:43.71 SC.reconfigure SCtight @15:00:43.77 SC.seek 3 @15:00:45.92 SC.reconfigure SCloose @15:00:46.15 Elbow.reconfigure TightElbow @15:00:46.21 Elbow.seek 3 @15:00:48.28 Forearm.reconfigure TightForearm @15:00:48.33 Forearm.seek 3 + 700 counts @15:00:49.38 SE.reconfigure SEpick @15:00:49.45 SE.seek beamBreak @15:00:51.77 SE.configure SEconfig @15:00:51.95 Elbow.seek 3 @15:00:52.16 Forearm.seek 3 + 300 counts @15:00:52.43 Forearm.reconfigure LooseForearm @15:00:52.48 Hand.close @15:00:53.10 SE.seek :down @15:00:53.34 Forearm.seek :retract @15:00:53.42 delay 3 seconds @15:00:54.34 Elbow.seek CC @15:00:55.19 SE.coast @15:00:56.05 Elbow.reconfigure LooseElbow @15:00:56.16 Forearm.reconfigure TightForearm @15:00:56.21 Forearm.seek CC @15:00:56.43 ATV.dial :waste2 @15:00:57.17 AS.reconfigure ASconfig @15:00:57.22 AS.seek :empty @15:00:57.55 CC.seek :closed @15:01:01.80 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.11 @15:01:01.82 Hand.open @15:01:02.52 Forearm.reconfigure LooseForearm @15:01:02.57 Forearm.seek :retract @15:01:02.89 ATV.dial :air2 @15:01:03.29 AS.reconfigure ASslow1 @15:01:03.34 AS.seek 1ml @15:01:04.04 SC.coast @15:01:04.18 collectSample 1500ml w/bubble point of 55psi @15:01:04.19 Priming 7ml @15:01:04.20 Intake.select :close @15:01:04.21 Exhaust.select :close @15:01:04.23 SS.hold @15:01:04.46 Calibrating pressure sensors @15:01:04.48 CTV.dial :air @15:01:05.89 SSV.dial :spit,avoiding: :exhaust @15:01:05.91 SS.reconfigure SSfast @15:01:05.96 SS.seek 5ml @15:01:08.56 SSV.dial CBV @15:01:08.59 CBV.dial SSV @15:01:10.09 delay 3.8 seconds @15:01:14.05 SSV.dial :spit @15:01:15.47 SS.seek 0ml @15:01:18.15 SSV.dial CBV @15:01:19.57 CTV.dial :intake @15:01:20.37 SSV.dial CBV @15:01:20.40 CBV.dial SSV @15:01:20.43 Allowing 40s for 7.0ml of remaining 7.0ml @15:01:20.44 Intake.select :open @15:01:22.62 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:01:22.64 SS.reconfigure SSinhale.with maxSpeed: 150 @15:01:22.69 SS.seek 7ml @15:01:27.71 Vacuum too high -- waiting up to 40s @15:01:31.38 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:01:31.40 SS.reconfigure SSinhale.with maxSpeed: 135 @15:01:31.45 SS.seek 7ml @15:01:32.23 Stroke completed -- waiting for pressure to equalize ... @15:01:39.89 Intake.select :close @15:01:40.05 SSV.dial :exhaust @15:01:40.87 Exhaust.select :open @15:01:40.98 SS.reconfigure SSexhale @15:01:41.03 SS.seek 0ml @15:01:45.01 Exhaust.select :close @15:01:45.13 Sampling 1500.0ml @15:01:45.15 Intake.select :close @15:01:45.16 Exhaust.select :close @15:01:45.17 SS.reconfigure SSconfig @15:01:45.21 SS.hold @15:01:46.00 CTV.dial :intake @15:01:46.02 SSV.dial CBV @15:01:46.08 CBV.dial SSV @15:01:46.87 Allowing 130s for 25.0ml of remaining 1500.0ml @15:01:46.88 Intake.select :open @15:01:49.07 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:01:49.09 SS.reconfigure SSinhale.with maxSpeed: 135 @15:01:49.14 SS.seek 25ml @15:01:52.20 Vacuum too high -- waiting up to 40s @15:01:53.82 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:01:53.84 SS.reconfigure SSinhale.with maxSpeed: 122 @15:01:53.89 SS.seek 25ml @15:01:56.67 Vacuum too high -- waiting up to 40s @15:01:58.32 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:01:58.34 SS.reconfigure SSinhale.with maxSpeed: 110 @15:01:58.39 SS.seek 25ml @15:02:00.86 Vacuum too high -- waiting up to 40s @15:02:02.25 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:02:02.27 SS.reconfigure SSinhale.with maxSpeed: 99 @15:02:02.32 SS.seek 25ml @15:02:04.74 Vacuum too high -- waiting up to 40s @15:02:06.12 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:02:06.14 SS.reconfigure SSinhale.with maxSpeed: 89 @15:02:06.20 SS.seek 25ml @15:02:08.61 Vacuum too high -- waiting up to 40s @15:02:09.97 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:02:09.99 SS.reconfigure SSinhale.with maxSpeed: 80 @15:02:10.04 SS.seek 25ml @15:02:12.83 Vacuum too high -- waiting up to 40s @15:02:14.19 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:02:14.20 SS.reconfigure SSinhale.with maxSpeed: 72 @15:02:14.25 SS.seek 25ml @15:02:17.39 Vacuum too high -- waiting up to 40s @15:02:19.13 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:02:19.16 SS.reconfigure SSinhale.with maxSpeed: 65 @15:02:19.21 SS.seek 25ml @15:02:23.11 Vacuum too high -- waiting up to 40s @15:02:24.22 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:02:24.25 SS.reconfigure SSinhale.with maxSpeed: 59 @15:02:24.29 SS.seek 25ml @15:02:28.74 Vacuum too high -- waiting up to 40s @15:02:30.11 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:02:30.13 SS.reconfigure SSinhale.with maxSpeed: 53 @15:02:30.18 SS.seek 25ml @15:02:32.36 Stroke completed -- waiting for pressure to equalize ... @15:02:34.97 Intake.select :close @15:02:35.13 SSV.dial :exhaust @15:02:35.93 Exhaust.select :open @15:02:36.05 SS.reconfigure SSexhale @15:02:36.10 SS.seek 0ml @15:02:45.25 Exhaust.select :close @15:02:45.37 SSV.dial CBV @15:02:45.40 CBV.dial SSV @15:02:46.24 Allowing 130s for 25.0ml of remaining 1475.0ml @15:02:46.25 Intake.select :open @15:02:48.43 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:02:48.45 SS.reconfigure SSinhale.with maxSpeed: 53 @15:02:48.50 SS.seek 25ml @15:02:57.33 Vacuum too high -- waiting up to 40s @15:02:58.42 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:02:58.44 SS.reconfigure SSinhale.with maxSpeed: 48 @15:02:58.49 SS.seek 25ml @15:03:15.71 Vacuum too high -- waiting up to 40s @15:03:16.83 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:03:16.85 SS.reconfigure SSinhale.with maxSpeed: 43 @15:03:16.91 SS.seek 25ml @15:03:29.45 Stroke completed -- waiting for pressure to equalize ... @15:03:32.05 Intake.select :close @15:03:32.21 SSV.dial :exhaust @15:03:33.02 Exhaust.select :open @15:03:33.13 SS.reconfigure SSexhale @15:03:33.18 SS.seek 0ml @15:03:42.08 Exhaust.select :close @15:03:42.20 SSV.dial CBV @15:03:42.23 CBV.dial SSV @15:03:43.07 Allowing 130s for 25.0ml of remaining 1450.0ml @15:03:43.08 Intake.select :open @15:03:45.27 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:03:45.83 SS.reconfigure SSinhale.with maxSpeed: 43 @15:03:45.89 SS.seek 25ml @15:04:24.95 delay 5 seconds @15:04:27.59 Stroke completed -- waiting for pressure to equalize ... @15:04:29.96 ATV.dial ASV @15:04:30.84 Intake.select :close @15:04:30.92 ASV.dial :SPEdelivery @15:04:30.95 AS.seek 0.02ml @15:04:31.06 SSV.dial :exhaust @15:04:31.90 Exhaust.select :open @15:04:32.01 SS.reconfigure SSexhale @15:04:32.06 SS.seek 0ml @15:04:41.17 Exhaust.select :close @15:04:41.29 SSV.dial CBV @15:04:41.32 CBV.dial SSV @15:04:42.16 Allowing 130s for 25.0ml of remaining 1425.0ml @15:04:42.17 Intake.select :open @15:04:44.38 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:04:44.42 SS.reconfigure SSinhale.with maxSpeed: 43 @15:04:44.49 SS.seek 25ml @15:05:25.84 Stroke completed -- waiting for pressure to equalize ... @15:05:29.10 Intake.select :close @15:05:29.26 SSV.dial :exhaust @15:05:30.06 Exhaust.select :open @15:05:30.18 SS.reconfigure SSexhale @15:05:30.23 SS.seek 0ml @15:05:39.47 Exhaust.select :close @15:05:39.59 SSV.dial CBV @15:05:39.62 CBV.dial SSV @15:05:40.46 Allowing 130s for 25.0ml of remaining 1400.0ml @15:05:40.47 Intake.select :open @15:05:42.65 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:05:42.69 SS.reconfigure SSinhale.with maxSpeed: 43 @15:05:42.79 SS.seek 25ml @15:06:24.13 Stroke completed -- waiting for pressure to equalize ... @15:06:27.98 Intake.select :close @15:06:28.14 SSV.dial :exhaust @15:06:28.95 Exhaust.select :open @15:06:29.07 SS.reconfigure SSexhale @15:06:29.12 SS.seek 0ml @15:06:38.29 Exhaust.select :close @15:06:38.41 SSV.dial CBV @15:06:38.44 CBV.dial SSV @15:06:39.27 Allowing 130s for 25.0ml of remaining 1375.0ml @15:06:39.28 Intake.select :open @15:06:41.46 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:06:41.48 SS.reconfigure SSinhale.with maxSpeed: 43 @15:06:41.53 SS.seek 25ml @15:07:22.54 Stroke completed -- waiting for pressure to equalize ... @15:07:26.40 Intake.select :close @15:07:27.03 SSV.dial :exhaust @15:07:27.95 Exhaust.select :open @15:07:28.06 SS.reconfigure SSexhale @15:07:28.11 SS.seek 0ml @15:07:37.37 Exhaust.select :close @15:07:37.49 SSV.dial CBV @15:07:37.52 CBV.dial SSV @15:07:38.37 Allowing 130s for 25.0ml of remaining 1350.0ml @15:07:38.38 Intake.select :open @15:07:40.57 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:07:40.59 SS.reconfigure SSinhale.with maxSpeed: 43 @15:07:40.64 SS.seek 25ml @15:07:48.29 delay 5 seconds @15:07:53.31 ATV.dial :air2 @15:07:54.33 AS.seek 0.52ml @15:08:07.14 Vacuum too high -- waiting up to 40s @15:08:08.26 Intake @33.2psia, vacuum from 20.0 to 24.0psi @15:08:08.28 SS.reconfigure SSinhale.with maxSpeed: 39 @15:08:08.33 SS.seek 25ml @15:08:25.23 Stroke completed -- waiting for pressure to equalize ... @15:08:28.45 Intake.select :close @15:08:28.62 SSV.dial :exhaust @15:08:29.44 Exhaust.select :open @15:08:29.55 SS.reconfigure SSexhale @15:08:29.60 SS.seek 0ml @15:08:38.84 Exhaust.select :close @15:08:38.96 SSV.dial CBV @15:08:38.99 CBV.dial SSV @15:08:39.84 Allowing 130s for 25.0ml of remaining 1325.0ml @15:08:39.85 Intake.select :open @15:08:42.03 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:08:42.05 SS.reconfigure SSinhale.with maxSpeed: 39 @15:08:42.10 SS.seek 25ml @15:09:27.54 Stroke completed -- waiting for pressure to equalize ... @15:09:30.78 Intake.select :close @15:09:30.93 SSV.dial :exhaust @15:09:31.75 Exhaust.select :open @15:09:31.87 SS.reconfigure SSexhale @15:09:31.92 SS.seek 0ml @15:09:35.17 delay 5 seconds @15:09:40.19 ATV.dial ASV @15:09:41.13 ASV.dial :wasteR @15:09:41.29 Exhaust.select :close @15:09:41.41 SSV.dial CBV @15:09:41.44 CBV.dial SSV @15:09:41.53 AS.seek 0.3ml @15:09:42.32 Allowing 130s for 25.0ml of remaining 1300.0ml @15:09:42.33 Intake.select :open @15:09:44.51 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:09:44.53 SS.reconfigure SSinhale.with maxSpeed: 39 @15:09:44.58 SS.seek 25ml @15:10:26.09 delay 20 seconds @15:10:30.23 Stroke completed -- waiting for pressure to equalize ... @15:10:34.18 Intake.select :close @15:10:34.88 SSV.dial :exhaust @15:10:35.71 Exhaust.select :open @15:10:35.83 SS.reconfigure SSexhale @15:10:35.88 SS.seek 0ml @15:10:44.68 Exhaust.select :close @15:10:44.80 SSV.dial CBV @15:10:44.83 CBV.dial SSV @15:10:45.68 Allowing 130s for 25.0ml of remaining 1275.0ml @15:10:45.69 Intake.select :open @15:10:46.11 AS.seek 0.07ml @15:10:47.87 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:10:47.89 SS.reconfigure SSinhale.with maxSpeed: 39 @15:10:47.94 SS.seek 25ml @15:11:32.65 delay 20 seconds @15:11:33.62 Stroke completed -- waiting for pressure to equalize ... @15:11:37.50 Intake.select :close @15:11:37.67 SSV.dial :exhaust @15:11:38.49 Exhaust.select :open @15:11:38.60 SS.reconfigure SSexhale @15:11:38.66 SS.seek 0ml @15:11:47.67 Exhaust.select :close @15:11:47.79 SSV.dial CBV @15:11:47.82 CBV.dial SSV @15:11:48.66 Allowing 130s for 25.0ml of remaining 1250.0ml @15:11:48.67 Intake.select :open @15:11:50.85 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:11:50.88 SS.reconfigure SSinhale.with maxSpeed: 39 @15:11:50.93 SS.seek 25ml @15:11:52.67 ATV.dial :waste2 @15:11:53.42 AS.reconfigure ASconfig @15:11:53.47 AS.seek :empty @15:11:57.86 Elution @15:11:57.88 ATV.dial :air1 @15:11:58.97 AS.reconfigure ASslow1 @15:11:59.02 AS.seek 0.049ml @15:12:09.50 delay 3 seconds @15:12:12.52 ATV.dial :ElutionWater @15:12:13.29 AS.seek 0.109ml @15:12:25.64 delay 3 seconds @15:12:28.67 ATV.dial ASV,via: :air2 @15:12:30.67 ASV.dial :SPEelution @15:12:31.25 AFV.select :carrierSolution @15:12:31.43 AS.reconfigure ASnorm @15:12:31.48 AS.seek 0.759ml @15:12:36.36 Stroke completed -- waiting for pressure to equalize ... @15:12:40.85 Intake.select :close @15:12:41.01 SSV.dial :exhaust @15:12:41.83 Exhaust.select :open @15:12:41.94 SS.reconfigure SSexhale @15:12:42.00 SS.seek 0ml @15:12:51.06 Exhaust.select :close @15:12:51.18 SSV.dial CBV @15:12:51.21 CBV.dial SSV @15:12:52.04 Allowing 130s for 25.0ml of remaining 1225.0ml @15:12:52.05 Intake.select :open @15:12:54.24 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:12:54.26 SS.reconfigure SSinhale.with maxSpeed: 39 @15:12:54.31 SS.seek 25ml @15:13:04.59 delay 3 seconds @15:13:07.61 AFV.select :holdingCoil @15:13:07.78 AS.reconfigure ASslow1 @15:13:07.83 AS.seek 0.474ml @15:13:39.65 Stroke completed -- waiting for pressure to equalize ... @15:13:44.05 Intake.select :close @15:13:44.22 SSV.dial :exhaust @15:13:45.04 Exhaust.select :open @15:13:45.15 SS.reconfigure SSexhale @15:13:45.21 SS.seek 0ml @15:13:54.45 Exhaust.select :close @15:13:54.57 SSV.dial CBV @15:13:54.60 CBV.dial SSV @15:13:55.43 Allowing 130s for 25.0ml of remaining 1200.0ml @15:13:55.44 Intake.select :open @15:13:57.62 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:13:57.64 SS.reconfigure SSinhale.with maxSpeed: 39 @15:13:57.69 SS.seek 25ml @15:14:05.42 delay 5 seconds @15:14:10.51 AS.reconfigure ASslow2 @15:14:10.57 AS.seek 0.439ml @15:14:40.55 Vacuum too high -- waiting up to 40s @15:14:41.93 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:14:41.95 SS.reconfigure SSinhale.with maxSpeed: 35 @15:14:42.00 SS.seek 25ml @15:14:45.23 Stroke completed -- waiting for pressure to equalize ... @15:14:46.19 delay 1:00 @15:14:48.48 Intake.select :close @15:14:48.64 SSV.dial :exhaust @15:14:49.44 Exhaust.select :open @15:14:49.55 SS.reconfigure SSexhale @15:14:49.60 SS.seek 0ml @15:14:58.97 Exhaust.select :close @15:14:59.09 SSV.dial CBV @15:14:59.12 CBV.dial SSV @15:14:59.96 Allowing 130s for 25.0ml of remaining 1175.0ml @15:14:59.97 Intake.select :open @15:15:02.15 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:15:02.17 SS.reconfigure SSinhale.with maxSpeed: 35 @15:15:02.22 SS.seek 25ml @15:15:46.22 pushing eluate to holding coil @15:15:46.29 AS.seek 0.279ml @15:15:52.58 Stroke completed -- waiting for pressure to equalize ... @15:15:56.43 Intake.select :close @15:15:56.59 SSV.dial :exhaust @15:15:57.39 Exhaust.select :open @15:15:57.51 SS.reconfigure SSexhale @15:15:57.56 SS.seek 0ml @15:16:06.65 Exhaust.select :close @15:16:06.77 SSV.dial CBV @15:16:06.80 CBV.dial SSV @15:16:07.62 Allowing 130s for 25.0ml of remaining 1150.0ml @15:16:07.63 Intake.select :open @15:16:09.81 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:16:09.83 SS.reconfigure SSinhale.with maxSpeed: 35 @15:16:09.88 SS.seek 25ml @15:17:00.34 Stroke completed -- waiting for pressure to equalize ... @15:17:04.78 Intake.select :close @15:17:04.94 SSV.dial :exhaust @15:17:05.77 Exhaust.select :open @15:17:05.88 SS.reconfigure SSexhale @15:17:05.94 SS.seek 0ml @15:17:14.76 Exhaust.select :close @15:17:14.88 SSV.dial CBV @15:17:14.91 CBV.dial SSV @15:17:15.74 Allowing 130s for 25.0ml of remaining 1125.0ml @15:17:15.75 Intake.select :open @15:17:17.93 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:17:17.95 SS.reconfigure SSinhale.with maxSpeed: 35 @15:17:18.00 SS.seek 25ml @15:18:08.75 Stroke completed -- waiting for pressure to equalize ... @15:18:13.24 Intake.select :close @15:18:13.40 SSV.dial :exhaust @15:18:14.21 Exhaust.select :open @15:18:14.32 SS.reconfigure SSexhale @15:18:14.37 SS.seek 0ml @15:18:23.70 Exhaust.select :close @15:18:23.82 SSV.dial CBV @15:18:23.85 CBV.dial SSV @15:18:24.69 Allowing 130s for 25.0ml of remaining 1100.0ml @15:18:24.70 Intake.select :open @15:18:26.88 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:18:26.90 SS.reconfigure SSinhale.with maxSpeed: 35 @15:18:26.95 SS.seek 25ml @15:18:27.67 delay 30 seconds @15:18:57.70 ATV.dial :waste,via: :air3 @15:18:59.31 AS.reconfigure ASconfig @15:18:59.36 AS.seek :empty @15:19:15.78 Parking Bleach in LysateSource line and SPEcolumn @15:19:15.79 ATV.dial :air3 @15:19:16.58 AS.reconfigure ASslow1 @15:19:16.64 AS.seek 0.029ml @15:19:17.18 Stroke completed -- waiting for pressure to equalize ... @15:19:22.35 Intake.select :close @15:19:22.51 SSV.dial :exhaust @15:19:22.99 ATV.dial :ElutionWater @15:19:23.34 Exhaust.select :open @15:19:23.45 SS.reconfigure SSexhale @15:19:23.55 SS.seek 0ml @15:19:23.67 AS.seek 0.059ml @15:19:29.96 ATV.dial :air3 @15:19:30.57 AS.seek 0.069ml @15:19:32.88 delay 2 seconds @15:19:33.01 Exhaust.select :close @15:19:33.13 SSV.dial CBV @15:19:33.16 CBV.dial SSV @15:19:33.99 Allowing 130s for 25.0ml of remaining 1075.0ml @15:19:34.00 Intake.select :open @15:19:34.90 ATV.dial :bleach @15:19:35.38 AS.seek 0.769ml @15:19:36.74 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:19:36.76 SS.reconfigure SSinhale.with maxSpeed: 35 @15:19:36.81 SS.seek 25ml @15:20:27.04 Stroke completed -- waiting for pressure to equalize ... @15:20:32.18 Intake.select :close @15:20:32.35 SSV.dial :exhaust @15:20:33.16 Exhaust.select :open @15:20:33.27 SS.reconfigure SSexhale @15:20:33.32 SS.seek 0ml @15:20:42.62 Exhaust.select :close @15:20:42.74 SSV.dial CBV @15:20:42.77 CBV.dial SSV @15:20:43.60 Allowing 130s for 25.0ml of remaining 1050.0ml @15:20:43.61 Intake.select :open @15:20:45.79 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:20:45.81 SS.reconfigure SSinhale.with maxSpeed: 35 @15:20:45.87 SS.seek 25ml @15:21:36.50 Stroke completed -- waiting for pressure to equalize ... @15:21:41.65 Intake.select :close @15:21:41.80 SSV.dial :exhaust @15:21:42.62 Exhaust.select :open @15:21:42.74 SS.reconfigure SSexhale @15:21:42.79 SS.seek 0ml @15:21:51.93 Exhaust.select :close @15:21:52.05 SSV.dial CBV @15:21:52.08 CBV.dial SSV @15:21:52.93 Allowing 130s for 25.0ml of remaining 1025.0ml @15:21:52.94 Intake.select :open @15:21:55.12 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:21:55.14 SS.reconfigure SSinhale.with maxSpeed: 35 @15:21:55.19 SS.seek 25ml @15:21:56.39 delay 3 seconds @15:21:59.40 ATV.dial :air3 @15:21:59.94 delay 3 seconds @15:22:03.03 AS.seek 0.794ml @15:22:08.34 delay 3 seconds @15:22:11.36 ATV.dial :bleach @15:22:11.96 AS.seek 0.779ml @15:22:15.22 delay 3 seconds @15:22:18.24 ATV.dial :AIV @15:22:19.55 AS.reconfigure ASconfig @15:22:19.60 AS.seek 0.015ml @15:22:27.64 Vacuum too high -- waiting up to 40s @15:22:29.26 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:22:29.28 SS.reconfigure SSinhale.with maxSpeed: 32 @15:22:29.33 SS.seek 25ml @15:22:49.43 Stroke completed -- waiting for pressure to equalize ... @15:22:53.86 Intake.select :close @15:22:54.03 SSV.dial :exhaust @15:22:54.86 Exhaust.select :open @15:22:54.98 SS.reconfigure SSexhale @15:22:55.04 SS.seek 0ml @15:23:03.80 ATV.dial :waste @15:23:04.47 Exhaust.select :close @15:23:04.59 SSV.dial CBV @15:23:04.62 CBV.dial SSV @15:23:05.31 AS.seek :empty @15:23:05.48 Allowing 130s for 25.0ml of remaining 1000.0ml @15:23:05.49 Intake.select :open @15:23:06.52 ATV.dial :air3 @15:23:07.20 AS.reconfigure ASslow1 @15:23:07.25 AS.seek 0.009ml @15:23:07.68 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:23:07.70 SS.reconfigure SSinhale.with maxSpeed: 32 @15:23:07.75 SS.seek 25ml @15:23:09.58 delay 2 seconds @15:23:11.59 ATV.dial :bleach @15:23:12.07 AS.seek 0.579ml @15:24:02.73 Stroke completed -- waiting for pressure to equalize ... @15:24:07.90 Intake.select :close @15:24:08.06 SSV.dial :exhaust @15:24:08.87 Exhaust.select :open @15:24:08.99 SS.reconfigure SSexhale @15:24:09.04 SS.seek 0ml @15:24:18.18 Exhaust.select :close @15:24:18.30 SSV.dial CBV @15:24:18.33 CBV.dial SSV @15:24:19.16 Allowing 130s for 25.0ml of remaining 975.0ml @15:24:19.17 Intake.select :open @15:24:21.35 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:24:21.37 SS.reconfigure SSinhale.with maxSpeed: 32 @15:24:21.42 SS.seek 25ml @15:25:06.95 delay 3 seconds @15:25:09.97 ATV.dial :air3 @15:25:10.50 delay 3 seconds @15:25:13.59 AS.seek 0.604ml @15:25:16.50 Stroke completed -- waiting for pressure to equalize ... @15:25:18.87 delay 3 seconds @15:25:21.88 ATV.dial :bleach @15:25:22.24 Intake.select :close @15:25:22.42 SSV.dial :exhaust @15:25:22.55 AS.seek 0.589ml @15:25:23.30 Exhaust.select :open @15:25:23.41 SS.reconfigure SSexhale @15:25:24.04 SS.seek 0ml @15:25:25.85 delay 3 seconds @15:25:28.86 ATV.dial :ASV @15:25:29.85 ASV.dial :wasteR @15:25:30.43 AS.seek 0ml @15:25:32.95 Exhaust.select :close @15:25:33.07 SSV.dial CBV @15:25:33.10 CBV.dial SSV @15:25:33.93 Allowing 130s for 25.0ml of remaining 950.0ml @15:25:33.94 Intake.select :open @15:25:36.12 Intake @34.8psia, vacuum from 20.0 to 24.0psi @15:25:36.14 SS.reconfigure SSinhale.with maxSpeed: 32 @15:25:36.19 SS.seek 25ml @15:26:11.39 Vacuum too high -- waiting up to 40s @15:26:12.77 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:26:12.79 SS.reconfigure SSinhale.with maxSpeed: 29 @15:26:12.84 SS.seek 25ml @15:26:35.61 Stroke completed -- waiting for pressure to equalize ... @15:26:40.78 Intake.select :close @15:26:40.93 SSV.dial :exhaust @15:26:41.76 Exhaust.select :open @15:26:41.87 SS.reconfigure SSexhale @15:26:41.93 SS.seek 0ml @15:26:51.33 Exhaust.select :close @15:26:51.45 SSV.dial CBV @15:26:51.48 CBV.dial SSV @15:26:52.34 Allowing 130s for 25.0ml of remaining 925.0ml @15:26:52.35 Intake.select :open @15:26:54.54 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:26:54.56 SS.reconfigure SSinhale.with maxSpeed: 29 @15:26:54.60 SS.seek 25ml @15:27:29.19 delay 1:00 @15:27:55.22 Stroke completed -- waiting for pressure to equalize ... @15:28:00.35 Intake.select :close @15:28:00.51 SSV.dial :exhaust @15:28:01.33 Exhaust.select :open @15:28:01.45 SS.reconfigure SSexhale @15:28:01.50 SS.seek 0ml @15:28:10.29 Exhaust.select :close @15:28:10.41 SSV.dial CBV @15:28:10.44 CBV.dial SSV @15:28:11.27 Allowing 130s for 25.0ml of remaining 900.0ml @15:28:11.28 Intake.select :open @15:28:13.47 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:28:13.48 SS.reconfigure SSinhale.with maxSpeed: 29 @15:28:13.53 SS.seek 25ml @15:28:29.21 AFV.select :carrierSolution @15:28:29.32 delay 2 seconds @15:28:31.35 AS.reconfigure ASconfig @15:28:31.40 AS.seek 1ml @15:29:14.43 Stroke completed -- waiting for pressure to equalize ... @15:29:20.16 Intake.select :close @15:29:20.32 SSV.dial :exhaust @15:29:21.13 Exhaust.select :open @15:29:21.24 SS.reconfigure SSexhale @15:29:21.30 SS.seek 0ml @15:29:29.16 delay 1 second @15:29:30.17 AFV.select :holdingCoil @15:29:30.28 delay 1 second @15:29:30.47 Exhaust.select :close @15:29:30.59 SSV.dial CBV @15:29:30.62 CBV.dial SSV @15:29:31.31 ATV.dial ASV @15:29:31.33 ASV.dial :wasteR @15:29:31.35 AS.reconfigure ASslow1 @15:29:31.40 AS.seek 0ml @15:29:31.48 Allowing 130s for 25.0ml of remaining 875.0ml @15:29:31.50 Intake.select :open @15:29:33.67 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:29:33.70 SS.reconfigure SSinhale.with maxSpeed: 29 @15:29:33.75 SS.seek 25ml @15:30:34.26 Stroke completed -- waiting for pressure to equalize ... @15:30:40.02 Intake.select :close @15:30:40.18 SSV.dial :exhaust @15:30:41.00 Exhaust.select :open @15:30:41.12 SS.reconfigure SSexhale @15:30:41.17 SS.seek 0ml @15:30:50.50 Exhaust.select :close @15:30:50.62 SSV.dial CBV @15:30:50.65 CBV.dial SSV @15:30:51.51 Allowing 130s for 25.0ml of remaining 850.0ml @15:30:51.52 Intake.select :open @15:30:53.70 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:30:53.72 SS.reconfigure SSinhale.with maxSpeed: 29 @15:30:53.77 SS.seek 25ml @15:31:53.95 Stroke completed -- waiting for pressure to equalize ... @15:32:00.33 Intake.select :close @15:32:00.49 SSV.dial :exhaust @15:32:01.32 Exhaust.select :open @15:32:01.43 SS.reconfigure SSexhale @15:32:01.48 SS.seek 0ml @15:32:10.75 Exhaust.select :close @15:32:10.87 SSV.dial CBV @15:32:10.90 CBV.dial SSV @15:32:11.74 Allowing 130s for 25.0ml of remaining 825.0ml @15:32:11.75 Intake.select :open @15:32:13.93 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:32:13.95 SS.reconfigure SSinhale.with maxSpeed: 29 @15:32:14.00 SS.seek 25ml @15:32:50.33 Vacuum too high -- waiting up to 40s @15:32:51.76 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:32:51.78 SS.reconfigure SSinhale.with maxSpeed: 26 @15:32:51.83 SS.seek 25ml @15:32:52.80 delay 20 seconds @15:33:12.82 ATV.dial :air3 @15:33:14.26 AS.seek 0.59ml @15:33:19.47 Stroke completed -- waiting for pressure to equalize ... @15:33:25.33 Intake.select :close @15:33:25.49 SSV.dial :exhaust @15:33:26.29 Exhaust.select :open @15:33:26.41 SS.reconfigure SSexhale @15:33:26.46 SS.seek 0ml @15:33:35.26 Exhaust.select :close @15:33:35.38 SSV.dial CBV @15:33:35.41 CBV.dial SSV @15:33:36.24 Allowing 130s for 25.0ml of remaining 800.0ml @15:33:36.25 Intake.select :open @15:33:38.44 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:33:38.46 SS.reconfigure SSinhale.with maxSpeed: 26 @15:33:38.50 SS.seek 25ml @15:34:46.26 Stroke completed -- waiting for pressure to equalize ... @15:34:52.03 Intake.select :close @15:34:52.19 SSV.dial :exhaust @15:34:53.01 Exhaust.select :open @15:34:53.13 SS.reconfigure SSexhale @15:34:53.18 SS.seek 0ml @15:35:01.98 Exhaust.select :close @15:35:02.10 SSV.dial CBV @15:35:02.13 CBV.dial SSV @15:35:02.98 Allowing 130s for 25.0ml of remaining 775.0ml @15:35:02.99 Intake.select :open @15:35:05.18 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:35:05.20 SS.reconfigure SSinhale.with maxSpeed: 26 @15:35:05.24 SS.seek 25ml @15:35:13.21 delay 3 seconds @15:35:16.23 ATV.dial :ASV @15:35:17.57 ASV.dial :wasteR @15:35:17.66 AS.seek 0.09ml @15:36:13.06 Stroke completed -- waiting for pressure to equalize ... @15:36:20.08 Intake.select :close @15:36:20.25 SSV.dial :exhaust @15:36:21.07 Exhaust.select :open @15:36:21.18 SS.reconfigure SSexhale @15:36:21.23 SS.seek 0ml @15:36:30.58 Exhaust.select :close @15:36:30.70 SSV.dial CBV @15:36:30.73 CBV.dial SSV @15:36:31.57 Allowing 130s for 25.0ml of remaining 750.0ml @15:36:31.58 Intake.select :open @15:36:33.77 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:36:33.79 SS.reconfigure SSinhale.with maxSpeed: 26 @15:36:33.83 SS.seek 25ml @15:36:58.50 delay 30 seconds @15:37:28.52 ATV.dial :extSrc @15:37:29.27 delay 3 seconds @15:37:32.29 ATV.dial :ElutionWater @15:37:33.37 AS.seek 0.12ml @15:37:39.68 ATV.dial :air3 @15:37:40.28 AS.seek 0.13ml @15:37:41.42 Stroke completed -- waiting for pressure to equalize ... @15:37:42.56 delay 2 seconds @15:37:44.62 ATV.dial :bleach @15:37:45.09 AS.seek 0.23ml @15:37:48.47 Intake.select :close @15:37:48.64 SSV.dial :exhaust @15:37:49.44 Exhaust.select :open @15:37:49.55 SS.reconfigure SSexhale @15:37:49.60 SS.seek 0ml @15:37:58.67 Exhaust.select :close @15:37:58.79 SSV.dial CBV @15:37:58.82 CBV.dial SSV @15:37:59.66 Allowing 130s for 25.0ml of remaining 725.0ml @15:37:59.67 Intake.select :open @15:38:01.85 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:38:01.87 SS.reconfigure SSinhale.with maxSpeed: 26 @15:38:01.92 SS.seek 25ml @15:38:05.38 delay 3 seconds @15:38:08.40 ATV.dial :air3 @15:38:08.94 delay 3 seconds @15:38:12.02 AS.seek 0.255ml @15:38:17.37 delay 3 seconds @15:38:20.40 ATV.dial :bleach @15:38:21.01 AS.seek 0.24ml @15:38:24.29 delay 3 seconds @15:38:27.31 ATV.dial :ASV @15:38:28.29 ASV.dial :SPEdelivery @15:38:28.65 AFV.select :carrierSolution @15:38:28.77 delay 2 seconds @15:38:30.80 AS.reconfigure ASconfig @15:38:30.85 AS.seek 0.5ml @15:38:46.08 delay 1 second @15:38:47.09 AFV.select :holdingCoil @15:38:47.25 AS.reconfigure ASslow1 @15:38:47.30 AS.seek 0.17ml @15:38:56.05 Vacuum too high -- waiting up to 40s @15:38:57.66 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:38:58.15 SS.reconfigure SSinhale.with maxSpeed: 23 @15:38:58.29 SS.seek 25ml @15:39:13.87 Stroke completed -- waiting for pressure to equalize ... @15:39:19.67 Intake.select :close @15:39:19.84 SSV.dial :exhaust @15:39:20.66 Exhaust.select :open @15:39:20.77 SS.reconfigure SSexhale @15:39:20.83 SS.seek 0ml @15:39:29.59 Exhaust.select :close @15:39:29.71 SSV.dial CBV @15:39:29.74 CBV.dial SSV @15:39:30.59 Allowing 130s for 25.0ml of remaining 700.0ml @15:39:30.60 Intake.select :open @15:39:32.78 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:39:32.80 SS.reconfigure SSinhale.with maxSpeed: 23 @15:39:32.85 SS.seek 25ml @15:39:53.97 SPE.hold 2:46:40,95C @15:39:54.03 delay 3:00 @15:40:48.90 Stroke completed -- waiting for pressure to equalize ... @15:40:55.31 Intake.select :close @15:40:55.47 SSV.dial :exhaust @15:40:56.31 Exhaust.select :open @15:40:56.43 SS.reconfigure SSexhale @15:40:56.48 SS.seek 0ml @15:41:05.87 Exhaust.select :close @15:41:05.99 SSV.dial CBV @15:41:06.02 CBV.dial SSV @15:41:06.87 Allowing 130s for 25.0ml of remaining 675.0ml @15:41:06.88 Intake.select :open @15:41:09.06 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:41:09.08 SS.reconfigure SSinhale.with maxSpeed: 23 @15:41:09.13 SS.seek 25ml @15:42:25.37 Stroke completed -- waiting for pressure to equalize ... @15:42:31.80 Intake.select :close @15:42:31.96 SSV.dial :exhaust @15:42:32.82 Exhaust.select :open @15:42:32.93 SS.reconfigure SSexhale @15:42:32.98 SS.seek 0ml @15:42:42.50 Exhaust.select :close @15:42:42.62 SSV.dial CBV @15:42:43.22 CBV.dial SSV @15:42:44.09 Allowing 130s for 25.0ml of remaining 650.0ml @15:42:44.10 Intake.select :open @15:42:46.28 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:42:46.30 SS.reconfigure SSinhale.with maxSpeed: 23 @15:42:46.35 SS.seek 25ml @15:42:54.07 AFV.select :carrierSolution @15:42:54.18 delay 2 seconds @15:42:56.21 AS.reconfigure ASconfig @15:42:56.26 AS.seek 1ml @15:43:44.24 delay 1 second @15:43:45.25 AFV.select :holdingCoil @15:43:45.36 delay 1 second @15:43:46.38 ASV.dial :wasteD @15:43:47.39 AS.seek 0ml @15:44:02.09 Stroke completed -- waiting for pressure to equalize ... @15:44:09.12 Intake.select :close @15:44:09.28 SSV.dial :exhaust @15:44:10.12 Exhaust.select :open @15:44:10.23 SS.reconfigure SSexhale @15:44:10.28 SS.seek 0ml @15:44:19.75 Exhaust.select :close @15:44:19.87 SSV.dial CBV @15:44:19.90 CBV.dial SSV @15:44:20.76 Allowing 130s for 25.0ml of remaining 625.0ml @15:44:20.77 Intake.select :open @15:44:22.95 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:44:22.97 SS.reconfigure SSinhale.with maxSpeed: 23 @15:44:23.02 SS.seek 25ml @15:44:45.16 Priming Eluate @15:44:45.17 AFV.select :carrierSolution @15:44:45.28 delay 2 seconds @15:44:47.31 AS.seek 0.5ml @15:45:16.34 delay 1 second @15:45:17.35 AFV.select :holdingCoil @15:45:17.46 ATV.dial ASV @15:45:17.47 ASV.dial :SPErecovery @15:45:18.05 Swish #1 of 2 @15:45:18.13 AS.reconfigure ASslow0 @15:45:18.18 AS.seek 0.6ml @15:45:28.66 AS.seek 0.5ml @15:45:38.97 Swish #2 of 2 @15:45:39.06 AS.seek 0.6ml @15:45:39.29 Stroke completed -- waiting for pressure to equalize ... @15:45:46.33 Intake.select :close @15:45:46.48 SSV.dial :exhaust @15:45:47.34 Exhaust.select :open @15:45:47.45 SS.reconfigure SSexhale @15:45:47.50 SS.seek 0ml @15:45:49.57 AS.seek 0.5ml @15:45:56.93 Exhaust.select :close @15:45:57.05 SSV.dial CBV @15:45:57.08 CBV.dial SSV @15:45:57.93 Allowing 130s for 25.0ml of remaining 600.0ml @15:45:57.94 Intake.select :open @15:45:59.91 delay 3 seconds @15:46:00.72 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:46:00.76 SS.reconfigure SSinhale.with maxSpeed: 23 @15:46:00.84 SS.seek 25ml @15:46:03.00 AS.reconfigure ASslow1 @15:46:03.05 AS.seek 0.684ml @15:46:40.35 delay 3 seconds @15:46:43.38 Eluate primed @15:46:43.39 ATV.dial :waste2 @15:46:43.99 AS.reconfigure ASnorm @15:46:44.04 AS.seek 0ml @15:47:09.30 Vacuum too high -- waiting up to 40s @15:47:10.91 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:47:10.93 SS.reconfigure SSinhale.with maxSpeed: 21 @15:47:10.98 SS.seek 25ml @15:47:18.86 SPE.coast @15:47:18.95 ASV.dial :SPErecovery @15:47:18.97 Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1315h150ml.pcr @15:47:18.99 Building PCR Slug from ASV with MM1 mix, PP2 primer @15:47:19.00 Priming with carrier @15:47:19.01 ATV.dial ARV @15:47:19.73 Stroke completed -- waiting for pressure to equalize ... @15:47:19.95 ARV.dial :carrier @15:47:20.33 AS.seek 0.5ml @15:47:26.28 Intake.select :close @15:47:26.44 SSV.dial :exhaust @15:47:27.25 Exhaust.select :open @15:47:27.37 SS.reconfigure SSexhale @15:47:27.42 SS.seek 0ml @15:47:36.65 Exhaust.select :close @15:47:36.77 SSV.dial CBV @15:47:36.80 CBV.dial SSV @15:47:37.63 Allowing 130s for 25.0ml of remaining 575.0ml @15:47:37.64 Intake.select :open @15:47:39.82 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:47:39.84 SS.reconfigure SSinhale.with maxSpeed: 21 @15:47:39.89 SS.seek 25ml @15:47:45.87 AFV.select :carrierSolution @15:47:45.99 delay 2 seconds @15:47:48.02 AS.reconfigure ASconfig @15:47:48.07 AS.seek 1ml @15:48:17.12 delay 1 second @15:48:18.13 AFV.select :holdingCoil @15:48:18.24 delay 1 second @15:48:19.26 ATV.dial :waste @15:48:20.70 AS.reconfigure ASnorm @15:48:20.75 AS.seek :empty @15:49:03.20 Stroke completed -- waiting for pressure to equalize ... @15:49:10.81 Intake.select :close @15:49:10.97 SSV.dial :exhaust @15:49:11.48 AFV.select :carrierSolution @15:49:11.60 delay 2 seconds @15:49:11.79 Exhaust.select :open @15:49:11.90 SS.reconfigure SSexhale @15:49:11.96 SS.seek 0ml @15:49:13.63 AS.reconfigure ASconfig @15:49:13.69 AS.seek 0.5ml @15:49:21.09 Exhaust.select :close @15:49:21.21 SSV.dial CBV @15:49:21.24 CBV.dial SSV @15:49:22.09 Allowing 130s for 25.0ml of remaining 550.0ml @15:49:22.10 Intake.select :open @15:49:24.28 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:49:24.30 SS.reconfigure SSinhale.with maxSpeed: 21 @15:49:24.35 SS.seek 25ml @15:49:42.81 delay 1 second @15:49:43.83 AFV.select :holdingCoil @15:49:43.94 Building Slug @15:49:43.95 ATV.dial :ARV @15:49:45.59 delay 2 seconds @15:49:47.61 ARV.dial :air1 @15:49:48.01 delay 2 seconds @15:49:50.10 AS.reconfigure ASslow2 @15:49:50.15 AS.seek 0.506ml @15:49:56.38 delay 2 seconds @15:49:58.40 ARV.dial :MM1 @15:49:59.07 delay 2 seconds @15:50:01.10 Adding MM1 @15:50:01.17 AS.seek 0.524ml @15:50:19.54 delay 25 seconds @15:50:44.56 ARV.dial :PP2 @15:50:44.95 Adding PP2 @15:50:44.96 delay 2 seconds @15:50:47.05 AS.seek 0.53ml @15:50:48.06 Stroke completed -- waiting for pressure to equalize ... @15:50:53.45 delay 10 seconds @15:50:55.76 Intake.select :close @15:50:55.93 SSV.dial :exhaust @15:50:56.73 Exhaust.select :open @15:50:56.85 SS.reconfigure SSexhale @15:50:56.90 SS.seek 0ml @15:51:03.48 ARV.dial :air2 @15:51:03.88 adding ARV air @15:51:03.89 delay 2 seconds @15:51:05.81 Exhaust.select :close @15:51:05.98 AS.seek 0.536ml @15:51:06.04 SSV.dial CBV @15:51:06.07 CBV.dial SSV @15:51:06.90 Allowing 130s for 25.0ml of remaining 525.0ml @15:51:06.91 Intake.select :open @15:51:09.09 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:51:09.11 SS.reconfigure SSinhale.with maxSpeed: 21 @15:51:09.16 SS.seek 25ml @15:51:12.16 delay 5 seconds @15:51:17.18 ARV.dial :carrier @15:51:18.40 delay 7 seconds @15:51:25.49 AS.seek 0.594ml @15:52:23.99 delay 5 seconds @15:52:29.01 ATV.dial :ASV @15:52:29.42 delay 2 seconds @15:52:31.51 AS.seek 0.6ml @15:52:32.83 Stroke completed -- waiting for pressure to equalize ... @15:52:37.72 delay 4 seconds @15:52:41.11 Intake.select :close @15:52:41.27 SSV.dial :exhaust @15:52:41.74 ATV.dial ARV @15:52:42.10 Exhaust.select :open @15:52:42.21 SS.reconfigure SSexhale @15:52:42.30 delay 2 seconds @15:52:42.32 SS.seek 0ml @15:52:44.32 ARV.dial :carrier @15:52:44.35 delay 2 seconds @15:52:46.44 AS.seek 0.65ml @15:52:51.53 Exhaust.select :close @15:52:51.65 SSV.dial CBV @15:52:51.68 CBV.dial SSV @15:52:52.52 Allowing 130s for 25.0ml of remaining 500.0ml @15:52:52.53 Intake.select :open @15:52:54.71 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:52:54.74 SS.reconfigure SSinhale.with maxSpeed: 21 @15:52:54.78 SS.seek 25ml @15:53:36.99 delay 4 seconds @15:53:41.01 ATV.dial PCR @15:53:41.60 ASV.dial :waste1 @15:53:42.36 ARV.dial :air1 @15:53:42.74 Positioning Slug by 0.324ml @15:53:42.83 AS.seek 0.326ml @15:53:56.24 Vacuum too high -- waiting up to 40s @15:53:58.13 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:53:58.15 SS.reconfigure SSinhale.with maxSpeed: 19 @15:53:58.20 SS.seek 25ml @15:54:22.75 Stroke completed -- waiting for pressure to equalize ... @15:54:30.44 Intake.select :close @15:54:30.60 SSV.dial :exhaust @15:54:31.43 Exhaust.select :open @15:54:31.54 SS.reconfigure SSexhale @15:54:31.59 SS.seek 0ml @15:54:40.98 Exhaust.select :close @15:54:41.10 SSV.dial CBV @15:54:41.70 CBV.dial SSV @15:54:42.59 Allowing 130s for 25.0ml of remaining 475.0ml @15:54:42.60 Intake.select :open @15:54:44.79 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:54:44.81 SS.reconfigure SSinhale.with maxSpeed: 19 @15:54:44.86 SS.seek 25ml @15:56:17.12 Stroke completed -- waiting for pressure to equalize ... @15:56:25.44 Intake.select :close @15:56:25.60 SSV.dial :exhaust @15:56:26.41 Exhaust.select :open @15:56:26.52 SS.reconfigure SSexhale @15:56:26.57 SS.seek 0ml @15:56:35.89 Exhaust.select :close @15:56:36.01 SSV.dial CBV @15:56:36.04 CBV.dial SSV @15:56:36.87 Allowing 130s for 25.0ml of remaining 450.0ml @15:56:36.88 Intake.select :open @15:56:39.07 Intake @34.8psia, vacuum from 20.0 to 24.0psi @15:56:39.09 SS.reconfigure SSinhale.with maxSpeed: 19 @15:56:39.13 SS.seek 25ml @15:58:11.17 Stroke completed -- waiting for pressure to equalize ... @15:58:20.10 Intake.select :close @15:58:20.26 SSV.dial :exhaust @15:58:21.09 Exhaust.select :open @15:58:21.21 SS.reconfigure SSexhale @15:58:21.27 SS.seek 0ml @15:58:30.66 Exhaust.select :close @15:58:30.78 SSV.dial CBV @15:58:30.81 CBV.dial SSV @15:58:31.65 Allowing 130s for 25.0ml of remaining 425.0ml @15:58:31.66 Intake.select :open @15:58:33.84 Intake @35.1psia, vacuum from 20.0 to 24.0psi @15:58:33.86 SS.reconfigure SSinhale.with maxSpeed: 19 @15:58:33.91 SS.seek 25ml @15:59:08.98 delay 5 seconds @15:59:14.00 ATV.dial ARV @15:59:14.75 ASV.dial :PCRlock @15:59:15.12 delay 5 seconds @15:59:20.15 Running Ss Profile60C350 thermocycling @15:59:20.16 PCR.program Profile60C350 @15:59:20.31 Profile60C350 should complete in < 1:48:05 @15:59:20.32 PCR.run 2:20:06.25 @15:59:39.36 Vacuum too high -- waiting up to 40s @15:59:41.09 Intake @34.8psia, vacuum from 20.0 to 24.0psi @15:59:41.11 SS.reconfigure SSinhale.with maxSpeed: 17 @15:59:41.16 SS.seek 25ml @16:00:11.29 Stroke completed -- waiting for pressure to equalize ... @16:00:20.26 Intake.select :close @16:00:20.42 SSV.dial :exhaust @16:00:21.23 Exhaust.select :open @16:00:21.35 SS.reconfigure SSexhale @16:00:21.42 SS.seek 0ml @16:00:30.87 Exhaust.select :close @16:00:30.99 SSV.dial CBV @16:00:31.02 CBV.dial SSV @16:00:31.85 Allowing 130s for 25.0ml of remaining 400.0ml @16:00:31.86 Intake.select :open @16:00:34.05 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:00:34.07 SS.reconfigure SSinhale.with maxSpeed: 17 @16:00:34.12 SS.seek 25ml @16:02:16.64 Stroke completed -- waiting for pressure to equalize ... @16:02:25.58 Intake.select :close @16:02:25.74 SSV.dial :exhaust @16:02:26.55 Exhaust.select :open @16:02:26.66 SS.reconfigure SSexhale @16:02:26.72 SS.seek 0ml @16:02:35.90 Exhaust.select :close @16:02:36.63 SSV.dial CBV @16:02:36.66 CBV.dial SSV @16:02:37.51 Allowing 130s for 25.0ml of remaining 375.0ml @16:02:37.52 Intake.select :open @16:02:39.71 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:02:39.73 SS.reconfigure SSinhale.with maxSpeed: 17 @16:02:39.78 SS.seek 25ml @16:04:22.73 Stroke completed -- waiting for pressure to equalize ... @16:04:32.97 Intake.select :close @16:04:33.13 SSV.dial :exhaust @16:04:33.96 Exhaust.select :open @16:04:34.07 SS.reconfigure SSexhale @16:04:34.12 SS.seek 0ml @16:04:43.20 Exhaust.select :close @16:04:43.32 SSV.dial CBV @16:04:43.35 CBV.dial SSV @16:04:44.19 Allowing 130s for 25.0ml of remaining 350.0ml @16:04:44.20 Intake.select :open @16:04:46.39 Intake @35.1psia, vacuum from 20.0 to 24.0psi @16:04:46.41 SS.reconfigure SSinhale.with maxSpeed: 17 @16:04:46.45 SS.seek 25ml @16:06:29.36 Stroke completed -- waiting for pressure to equalize ... @16:06:40.18 Intake.select :close @16:06:40.34 SSV.dial :exhaust @16:06:41.15 Exhaust.select :open @16:06:41.27 SS.reconfigure SSexhale @16:06:41.32 SS.seek 0ml @16:06:50.43 Exhaust.select :close @16:06:50.55 SSV.dial CBV @16:06:50.58 CBV.dial SSV @16:06:51.41 Allowing 130s for 25.0ml of remaining 325.0ml @16:06:51.42 Intake.select :open @16:06:53.61 Intake @35.1psia, vacuum from 20.0 to 24.0psi @16:06:53.63 SS.reconfigure SSinhale.with maxSpeed: 17 @16:06:53.68 SS.seek 25ml @16:08:11.42 Vacuum too high -- waiting up to 40s @16:08:13.29 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:08:13.31 SS.reconfigure SSinhale.with maxSpeed: 15 @16:08:13.36 SS.seek 25ml @16:08:42.06 Stroke completed -- waiting for pressure to equalize ... @16:08:51.77 Intake.select :close @16:08:51.93 SSV.dial :exhaust @16:08:52.77 Exhaust.select :open @16:08:52.88 SS.reconfigure SSexhale @16:08:52.93 SS.seek 0ml @16:09:02.37 Exhaust.select :close @16:09:02.49 SSV.dial CBV @16:09:02.52 CBV.dial SSV @16:09:03.35 Allowing 130s for 25.0ml of remaining 300.0ml @16:09:03.36 Intake.select :open @16:09:05.54 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:09:05.56 SS.reconfigure SSinhale.with maxSpeed: 15 @16:09:05.61 SS.seek 25ml @16:11:02.29 Stroke completed -- waiting for pressure to equalize ... @16:11:13.03 Intake.select :close @16:11:13.20 SSV.dial :exhaust @16:11:14.01 Exhaust.select :open @16:11:14.12 SS.reconfigure SSexhale @16:11:14.17 SS.seek 0ml @16:11:23.43 Exhaust.select :close @16:11:23.55 SSV.dial CBV @16:11:23.58 CBV.dial SSV @16:11:24.41 Allowing 130s for 25.0ml of remaining 275.0ml @16:11:24.42 Intake.select :open @16:11:26.60 Intake @35.1psia, vacuum from 20.0 to 24.0psi @16:11:26.62 SS.reconfigure SSinhale.with maxSpeed: 15 @16:11:26.67 SS.seek 25ml @16:13:23.22 Stroke completed -- waiting for pressure to equalize ... @16:13:34.10 Intake.select :close @16:13:34.26 SSV.dial :exhaust @16:13:35.08 Exhaust.select :open @16:13:35.19 SS.reconfigure SSexhale @16:13:35.24 SS.seek 0ml @16:13:44.65 Exhaust.select :close @16:13:44.77 SSV.dial CBV @16:13:45.32 CBV.dial SSV @16:13:46.26 Allowing 130s for 25.0ml of remaining 250.0ml @16:13:46.27 Intake.select :open @16:13:48.46 Intake @35.0psia, vacuum from 20.0 to 24.0psi @16:13:48.48 SS.reconfigure SSinhale.with maxSpeed: 15 @16:13:48.53 SS.seek 25ml @16:15:45.37 Stroke completed -- waiting for pressure to equalize ... @16:15:56.28 SS.reconfigure SSconfig @16:15:56.33 SS.hold @16:15:56.47 Intake.select :close @16:15:56.63 ShallowSampler::Clogged in WCR -- progress too slow after 1275.0ml @16:15:56.64 SSV.dial :exhaust @16:15:57.46 Exhaust.select :open @16:15:57.57 SS.reconfigure SSexhale @16:15:57.63 SS.seek 0ml @16:16:06.64 Exhaust.select :close @16:16:06.79 Sampled 1275.0ml @16:16:06.81 Drying puck before fixing for 4:00 @16:16:06.82 CBV.dial :waste2b @16:16:07.64 delay 1:00 @16:17:07.66 CSV.dial :air @16:17:08.14 CS.reconfigure CSfast @16:17:08.20 CS.seek 10ml @16:17:33.07 Pumping wcr puck from tube 3 up to 55psi... @16:17:33.08 CBV.dial SSV @16:17:34.47 CBV.dial between CSV and waste2b @16:17:35.53 CSV.dial between air and CTV @16:17:35.89 CS.seek 6.773ml @16:17:44.69 CTV.dial CSV,avoiding: :air @16:17:44.72 CSV.dial CTV @16:17:45.58 CS.reconfigure CSpressurize @16:17:45.64 CS.seek 0ml @16:18:01.56 CBV.dial :waste2b @16:18:01.91 CS.reconfigure CSconfig @16:18:01.96 CS.seek :empty @16:18:04.91 delay 1:00 @16:19:04.94 CTV.dial :air,avoiding: :intake @16:19:06.35 Fix #1 of 2 (with rnal) for 2:00 @16:19:06.37 CSV.dial CSR @16:19:07.02 Pulling 2ml of CSR.rnal @16:19:07.03 CSR.select :rnal @16:19:07.16 CS.seek 2ml @16:19:13.38 delay 5 seconds @16:19:18.40 CSR.select :air @16:19:18.53 CS.reconfigure CSfast @16:19:18.59 CS.seek 10ml @16:19:38.60 Pumping wcr puck from tube 3 up to 55psi... @16:19:38.61 CBV.dial SSV @16:19:39.99 CBV.dial between CSV and waste2b @16:19:41.03 CSV.dial between air and CTV @16:19:41.80 CS.seek 6.784ml @16:19:50.41 CTV.dial CSV,avoiding: :air @16:19:50.44 CSV.dial CTV @16:19:51.91 CS.reconfigure CSpressurize @16:19:51.97 CS.seek 0ml @16:20:08.87 delay 2:00 @16:22:08.89 CBV.dial :waste2b @16:22:09.26 CS.reconfigure CSconfig @16:22:09.31 CS.seek :empty @16:22:11.23 delay 1:00 @16:23:11.26 CTV.dial :air,avoiding: :intake @16:23:12.66 Fix #2 of 2 (with rnal) for 2:00 @16:23:12.68 CSV.dial CSR @16:23:13.21 Pulling 2ml of CSR.rnal @16:23:13.22 CSR.select :rnal @16:23:13.35 CS.seek 2ml @16:23:19.57 delay 5 seconds @16:23:24.59 CSR.select :air @16:23:24.72 CS.reconfigure CSfast @16:23:24.78 CS.seek 10ml @16:23:44.70 Pumping wcr puck from tube 3 up to 55psi... @16:23:44.71 CBV.dial SSV @16:23:46.07 CBV.dial between CSV and waste2b @16:23:47.12 CSV.dial between air and CTV @16:23:48.36 CS.seek 6.693ml @16:23:57.16 CTV.dial CSV,avoiding: :air @16:23:57.19 CSV.dial CTV @16:23:58.67 CS.reconfigure CSpressurize @16:23:58.73 CS.seek 0ml @16:24:14.81 delay 2:00 @16:26:14.83 CBV.dial :waste2b @16:26:15.18 CS.reconfigure CSconfig @16:26:15.23 CS.seek :empty @16:26:17.89 delay 1:00 @16:27:17.91 CTV.dial :air,avoiding: :intake @16:27:19.38 Storing wcr puck in Collection Clamp to tube 2 @16:27:19.58 SC.reconfigure SCtight @16:27:19.63 SC.seek 2 @16:27:21.79 SC.reconfigure SCloose @16:27:22.03 Elbow.reconfigure TightElbow @16:27:22.08 Elbow.seek 2 @16:27:23.92 Forearm.reconfigure TightForearm @16:27:23.98 Forearm.seek 2 + 700 counts @16:27:25.20 SE.reconfigure SEpick @16:27:25.27 SE.seek beamBreak @16:27:27.19 SE.configure SEconfig @16:27:27.37 Elbow.reconfigure LimpElbow @16:27:27.43 Forearm.reconfigure LooseForearm @16:27:27.48 Forearm.seek 2 @16:27:27.79 Elbow.hold @16:27:27.87 Elbow.reconfigure TightElbow @16:27:27.98 SE.seek 12.08 Pucks below Top Plate @16:27:28.46 Forearm.force -20 @16:27:28.51 Forearm.seek 2 + 344 counts @16:27:28.87 Forearm.reconfigure TightForearm @16:27:28.93 Forearm.seek 2 + 700 counts @16:27:29.16 SE.seek 13.08 Pucks below Top Plate @16:27:29.84 Forearm.reconfigure LooseForearm @16:27:29.89 Forearm.seek :retract @16:27:30.84 Elbow.seek CC @16:27:33.19 Elbow.reconfigure LooseElbow @16:27:33.34 Forearm.seek CC @16:27:34.30 Hand.close @16:27:34.95 CC.seek :open @16:27:39.50 Forearm.seek :retract @16:27:40.50 Elbow.reconfigure TightElbow @16:27:40.55 Elbow.seek 2 @16:27:42.42 Elbow.reconfigure LimpElbow @16:27:42.47 Forearm.seek 2 @16:27:43.51 Elbow.hold @16:27:43.59 Elbow.reconfigure TightElbow @16:27:43.64 Hand.open @16:27:44.47 Forearm.force -20 @16:27:44.52 Forearm.seek 2 + 335 counts @16:27:44.86 Forearm.reconfigure TightForearm @16:27:44.91 Forearm.seek 2 + 700 counts @16:27:45.30 SE.seek :down @16:27:46.46 Forearm.reconfigure LooseForearm @16:27:46.51 Forearm.seek :retract @16:27:46.85 SE.coast @16:27:47.45 SC.coast @16:27:47.49 Starting finalPurge @16:27:47.51 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:27:47.59 Hand.open @16:27:47.97 Elbow.seek FlushPuck::Garage @16:27:50.00 Elbow.reconfigure LooseElbow @16:27:50.11 Forearm.seek :garage @16:27:51.17 Hand.close @16:27:51.96 Forearm.seek :retract @16:27:52.95 Elbow.reconfigure TightElbow @16:27:53.00 Elbow.seek CC @16:27:55.96 Elbow.reconfigure LooseElbow @16:27:56.07 Forearm.reconfigure TightForearm @16:27:56.13 Forearm.seek CC @16:27:57.42 CC.seek :closed @16:28:02.21 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.08 @16:28:02.38 Hand.open @16:28:03.02 Forearm.reconfigure LooseForearm @16:28:03.07 Forearm.seek :retract @16:28:04.04 Flushing the Collection Valve::Manifold @16:28:04.18 CSV.dial CSR @16:28:04.71 Pulling 5ml of CSR.flush @16:28:04.72 CSR.select :flush @16:28:04.85 CS.seek 5ml @16:28:18.67 delay 5 seconds @16:28:23.69 CSR.select :air @16:28:23.86 CS.reconfigure CSfast @16:28:23.91 CS.seek 7.5ml @16:28:30.85 CSV.dial :waste @16:28:32.25 CS.reconfigure CSconfig @16:28:32.31 CS.seek 0ml @16:28:52.51 Flushing lower manifold's waste1 port @16:28:52.66 CSV.dial :flush @16:28:53.15 CS.reconfigure CSfast @16:28:53.20 CS.seek 5ml @16:29:06.01 delay 5 seconds @16:29:11.03 CSV.dial :air @16:29:11.58 CS.seek 7ml @16:29:17.36 CSV.dial CTV @16:29:17.39
CTV.dial CSV @16:29:18.86 CBV.dial :waste1 @16:29:19.69 CS.seek 0ml @16:29:37.08 Flushing lower manifold's waste2b port @16:29:37.22 CSV.dial :flush @16:29:38.04 CS.seek 5ml @16:29:50.76 delay 5 seconds @16:29:55.77 CSV.dial :air @16:29:56.37 CS.seek 7ml @16:30:02.20 CSV.dial CTV @16:30:02.23
CTV.dial CSV @16:30:02.70 CBV.dial :waste2b @16:30:03.54 CS.seek 0ml @16:30:20.91 Flushing upper manifold @16:30:21.05 CSV.dial :flush @16:30:21.85 CS.seek 5ml @16:30:34.64 delay 5 seconds @16:30:39.65 CSV.dial :air @16:30:40.22 CS.seek 7ml @16:30:46.05 CSV.dial CBV @16:30:46.08
CBV.dial CSV @16:30:47.25 CTV.dial :air @16:30:48.65 CS.seek 0ml @16:31:06.08 delay 7 seconds @16:31:13.10 CS.seek 3ml @16:31:21.22 CSV.dial :waste @16:31:21.72 CS.seek 0ml @16:31:29.79 CSV.dial :flush @16:31:30.86 CS.seek 3ml @16:31:38.95 delay 5 seconds @16:31:43.96 CSV.dial :air @16:31:44.52 CS.seek 5ml @16:31:50.47 CSV.dial :air @16:31:50.50 CS.seek 0ml @16:32:03.23 delay 7 seconds @16:32:10.25 CS.seek 3ml @16:32:18.29 CSV.dial :waste @16:32:19.10 CS.seek 0ml @16:32:27.20 Disinfecting external Intake with mfbkill @16:32:27.34 CSV.dial CSR @16:32:28.78 Pulling 8ml of CSR.mfbkill @16:32:28.79 CSR.select :mfbkill @16:32:28.92 CS.seek 8ml @16:32:48.89 delay 5 seconds @16:32:53.91 CSR.select :air @16:32:54.04 CS.seek 10ml @16:32:59.74 CSV.dial CBV @16:32:59.77
CBV.dial CSV @16:33:00.88 CTV.dial :intake @16:33:01.67 Intake.select :open @16:33:01.79 CS.seek 2ml @16:33:22.44 Intake.select :close @16:33:22.56 CTV.dial :air @16:33:23.36 CS.reconfigure CSconfig @16:33:23.41 CS.seek 0ml @16:33:29.45 Flushing lower manifold's waste1 port @16:33:29.46 CSV.dial CSR @16:33:30.61 Pulling 4ml of CSR.mfbkill @16:33:30.62 CSR.select :mfbkill @16:33:30.76 CS.seek 4ml @16:33:41.92 delay 5 seconds @16:33:46.95 CSR.select :air @16:33:47.07 CS.reconfigure CSfast @16:33:47.12 CS.seek 10ml @16:34:02.14 CSV.dial CTV @16:34:02.17
CTV.dial CSV @16:34:03.66 CBV.dial :waste1 @16:34:04.17 CS.seek 0ml @16:34:28.58 Flushing lower manifold's waste2b port @16:34:28.59 CSV.dial CSR @16:34:29.14 Pulling 4ml of CSR.mfbkill @16:34:29.15 CSR.select :mfbkill @16:34:29.28 CS.reconfigure CSconfig @16:34:29.32 CS.seek 4ml @16:34:40.49 delay 5 seconds @16:34:45.53 CSR.select :air @16:34:45.65 CS.reconfigure CSfast @16:34:45.70 CS.seek 10ml @16:35:00.83 CSV.dial CTV @16:35:00.86
CTV.dial CSV @16:35:01.36 CBV.dial :waste2b @16:35:02.20 CS.seek 0ml @16:35:26.43 Killing upper manifold @16:35:26.44 CSV.dial CSR @16:35:27.00 Pulling 4ml of CSR.mfbkill @16:35:27.01 CSR.select :mfbkill @16:35:27.14 CS.reconfigure CSconfig @16:35:27.18 CS.seek 4ml @16:35:38.39 delay 5 seconds @16:35:43.40 CSR.select :air @16:35:43.53 CS.reconfigure CSfast @16:35:44.18 CS.seek 10ml @16:35:59.14 CSV.dial CBV @16:35:59.17
CBV.dial CSV @16:36:00.30 CTV.dial :air @16:36:01.72 CS.seek 0ml @16:36:26.24 delay 7 seconds @16:36:33.26 CS.seek 3ml @16:36:41.39 CSV.dial :waste @16:36:41.89 CS.seek 0ml @16:36:49.96 Flushing upper manifold @16:36:50.12 CSV.dial :flush @16:36:50.61 CS.seek 5ml @16:37:03.24 delay 5 seconds @16:37:08.25 CSV.dial :air @16:37:08.92 CS.seek 7ml @16:37:14.71 CSV.dial CBV @16:37:14.74
CBV.dial CSV @16:37:15.82 CTV.dial :air @16:37:15.84 CS.seek 0ml @16:37:33.33 delay 7 seconds @16:37:40.36 CS.seek 3ml @16:37:48.45 CSV.dial :waste @16:37:49.07 CS.seek 0ml @16:37:57.23 CSV.dial :flush @16:37:57.73 CS.seek 3ml @16:38:05.77 delay 5 seconds @16:38:10.78 CSV.dial :air @16:38:11.34 CS.seek 5ml @16:38:17.04 CSV.dial :air @16:38:17.07 CS.seek 0ml @16:38:30.21 delay 7 seconds @16:38:37.23 CS.seek 3ml @16:38:45.36 CSV.dial :waste @16:38:46.17 CS.seek 0ml @16:38:54.18 Flushing lower manifold's waste1 port @16:38:54.32 CSV.dial :flush @16:38:54.82 CS.seek 5ml @16:39:07.65 delay 5 seconds @16:39:12.66 CSV.dial :air @16:39:13.33 CS.seek 7ml @16:39:19.02 CSV.dial CTV @16:39:19.06
CTV.dial CSV @16:39:20.50 CBV.dial :waste1 @16:39:21.02 CS.seek 0ml @16:39:38.30 Flushing lower manifold's waste2b port @16:39:38.44 CSV.dial :flush @16:39:39.24 CS.seek 5ml @16:39:51.90 delay 5 seconds @16:39:56.91 CSV.dial :air @16:39:57.48 CS.seek 7ml @16:40:03.27 CSV.dial CTV @16:40:03.30
CTV.dial CSV @16:40:04.38 CBV.dial :waste2b @16:40:05.22 CS.seek 0ml @16:40:22.65 Disinfecting external Intake with kill @16:40:22.79 CSV.dial CSR @16:40:23.33 Pulling 8ml of CSR.kill @16:40:23.34 CSR.select :kill @16:40:23.47 CS.seek 8ml @16:40:43.24 delay 5 seconds @16:40:48.26 CSR.select :air @16:40:48.40 CS.seek 10ml @16:40:54.09 CSV.dial CBV @16:40:54.12
CBV.dial CSV @16:40:55.26 CTV.dial :intake @16:40:56.06 Intake.select :open @16:40:56.18 CS.seek 2ml @16:41:16.92 Intake.select :close @16:41:17.04 CTV.dial :air @16:41:17.84 CS.reconfigure CSconfig @16:41:17.89 CS.seek 0ml @16:41:24.01 CSV.dial CSR @16:41:25.16 Pulling 5ml of CSR.flush @16:41:25.17 CSR.select :flush @16:41:25.30 CS.seek 5ml @16:41:39.09 delay 5 seconds @16:41:44.11 CSR.select :air @16:41:44.25 CS.seek 10ml @16:41:58.08 CSV.dial CBV @16:41:59.18 CS.seek 0ml @16:42:25.78 Garaging FlushPuck in Collection Clamp @16:42:25.86 Elbow.reconfigure TightElbow @16:42:25.91 Elbow.seek CC @16:42:26.20 Elbow.reconfigure LooseElbow @16:42:26.31 Forearm.seek CC @16:42:27.27 Hand.close @16:42:27.85 CC.seek :open @16:42:32.22 Forearm.seek :retract @16:42:33.21 Elbow.reconfigure TightElbow @16:42:33.26 Elbow.seek :garage @16:42:36.33 Elbow.reconfigure LooseElbow @16:42:36.44 Forearm.reconfigure TightForearm @16:42:36.49 Forearm.seek :garage @16:42:37.68 Hand.open @16:42:38.42 Forearm.reconfigure LooseForearm @16:42:38.47 Forearm.seek :retract @16:42:39.52 Starting initialPurge @16:42:39.53 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:42:39.61 Hand.open @16:42:39.91 Elbow.reconfigure TightElbow @16:42:39.96 Elbow.seek FlushPuck::Garage @16:42:40.22 Elbow.reconfigure LooseElbow @16:42:40.33 Forearm.seek :garage @16:42:41.36 Hand.close @16:42:42.10 Forearm.seek :retract @16:42:43.13 Elbow.reconfigure TightElbow @16:42:43.18 Elbow.seek CC @16:42:46.75 Elbow.reconfigure LooseElbow @16:42:46.85 Forearm.reconfigure TightForearm @16:42:46.90 Forearm.seek CC @16:42:48.19 CC.seek :closed @16:42:52.44 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.10 @16:42:52.53 Hand.open @16:42:53.30 Forearm.reconfigure LooseForearm @16:42:53.34 Forearm.seek :retract @16:42:54.28 Flushing the Collection Valve::Manifold @16:42:54.42 CSV.dial CSR @16:42:55.57 Pulling 5ml of CSR.flush @16:42:55.58 CSR.select :flush @16:42:55.71 CS.seek 5ml @16:43:09.49 delay 5 seconds @16:43:14.51 CSR.select :air @16:43:14.69 CS.reconfigure CSfast @16:43:14.74 CS.seek 7.5ml @16:43:21.64 CSV.dial :waste @16:43:23.03 CS.reconfigure CSconfig @16:43:23.08 CS.seek 0ml @16:43:43.18 Purging external Intake @16:43:43.31 CSV.dial :flush @16:43:43.81 CS.reconfigure CSfast @16:43:43.86 CS.seek 10ml @16:44:08.28 delay 5 seconds @16:44:13.30 CSV.dial CBV @16:44:13.34
CBV.dial CSV @16:44:14.14 CTV.dial :intake @16:44:14.94 Intake.select :open @16:44:15.07 CS.seek 0ml @16:44:40.61 Intake.select :close @16:44:40.73 CTV.dial :air @16:44:41.54 Flushing upper manifold @16:44:41.67 CSV.dial :flush @16:44:42.47 CS.seek 5ml @16:44:55.25 delay 5 seconds @16:45:00.27 CSV.dial :air @16:45:00.94 CS.seek 7ml @16:45:06.59 CSV.dial CBV @16:45:06.62
CBV.dial CSV @16:45:07.70 CTV.dial :air @16:45:07.72 CS.seek 0ml @16:45:25.00 delay 7 seconds @16:45:32.02 CS.seek 3ml @16:45:40.17 CSV.dial :waste @16:45:40.78 CS.seek 0ml @16:45:48.96 CSV.dial :flush @16:45:49.45 CS.seek 3ml @16:45:57.56 delay 5 seconds @16:46:02.57 CSV.dial :air @16:46:03.24 CS.seek 5ml @16:46:08.89 CSV.dial :air @16:46:08.92 CS.seek 0ml @16:46:22.25 delay 7 seconds @16:46:29.28 CS.seek 3ml @16:46:37.24 CSV.dial :waste @16:46:38.04 CS.seek 0ml @16:46:46.06 Garaging FlushPuck in Collection Clamp @16:46:46.14 Elbow.reconfigure TightElbow @16:46:46.19 Elbow.seek CC @16:46:46.46 Elbow.reconfigure LooseElbow @16:46:46.57 Forearm.seek CC @16:46:47.55 Hand.close @16:46:48.23 CC.seek :open @16:46:52.63 Forearm.seek :retract @16:46:53.65 Elbow.reconfigure TightElbow @16:46:53.70 Elbow.seek :garage @16:46:56.77 Elbow.reconfigure LooseElbow @16:46:56.88 Forearm.reconfigure TightForearm @16:46:56.93 Forearm.seek :garage @16:46:58.09 Hand.open @16:46:58.80 Forearm.reconfigure LooseForearm @16:46:58.85 Forearm.seek :retract @16:46:59.83 Completed [mbaPhase] begun 14:00:51 @16:46:59.85 Preparing to sleep until Mon Oct 15 14:00:51 +0200 2018 @16:46:59.90 deferred until MFBPCR finish @16:46:59.93
Waiting for MFBPCR til 14:00CEST15-Oct-18 @16:46:59.94 Continuing at 14:00CEST15-Oct-18 @16:47:00.01 GoodNews.email "Then, in tube 3: mbaPhase\n/home/esp/esp2/mission/18tundeployv3.rb:123\n{1 => 22, 2 => 14, 3 => 8}\nCan@16:46:59, 14.4C, 70% humidity, 14.3psia, 13.068V, 0.682A, 0.682A avg, 8.92W",Subject: "Waiting until 14:00CEST15-Oct-18" @17:22:08.57 PCR.off @17:22:10.62 PCR for Ss completed -- closed /var/log/esp/Ss18oct1315h150ml.pcr @17:22:10.70 Cleaning PCR @17:22:10.71 AFV.select :carrierSolution @17:22:10.82 delay 2 seconds @17:22:12.85 AS.reconfigure ASconfig @17:22:12.90 AS.seek 0.5ml @17:22:23.19 delay 1 second @17:22:24.20 AFV.select :holdingCoil @17:22:24.31 ASV.dial :waste1 @17:22:24.69 ATV.dial :air3 @17:22:25.90 AS.reconfigure ASslow1 @17:22:25.95 AS.seek 0.51ml @17:22:28.25 delay 2 seconds @17:22:30.26 ATV.dial :bleach @17:22:30.86 AS.seek 0.81ml @17:23:31.46 delay 3 seconds @17:23:34.48 ATV.dial :air3 @17:23:35.01 delay 3 seconds @17:23:38.10 AS.seek 0.835ml @17:23:43.42 delay 3 seconds @17:23:46.44 ATV.dial :bleach @17:23:47.04 AS.seek 0.82ml @17:23:50.36 delay 3 seconds @17:23:53.38 ARV.dial :waste @17:23:53.97 delay 3 seconds @17:23:56.99 ATV.dial :ARV @17:23:57.95 AS.reconfigure ASconfig @17:23:58.00 AS.seek 0.47ml @17:24:18.40 AFV.select :carrierSolution @17:24:18.52 delay 2 seconds @17:24:20.55 AS.seek 1ml @17:24:51.30 delay 1 second @17:24:52.31 AFV.select :holdingCoil @17:24:52.42 delay 1 second @17:24:53.45 AS.reconfigure ASfast @17:24:53.50 AS.seek 0ml @17:25:20.82 AFV.select :carrierSolution @17:25:20.94 delay 2 seconds @17:25:22.97 AS.reconfigure ASconfig @17:25:23.02 AS.seek 1ml @17:26:20.77 delay 1 second @17:26:21.78 AFV.select :holdingCoil @17:26:21.89 delay 1 second @17:26:22.92 AS.reconfigure ASfast @17:26:23.54 AS.seek 0ml @17:26:50.86 AFV.select :carrierSolution @17:26:50.98 delay 2 seconds @17:26:53.01 AS.reconfigure ASconfig @17:26:53.06 AS.seek 1ml @17:27:50.85 delay 1 second @17:27:51.86 AFV.select :holdingCoil @17:27:51.97 delay 1 second @17:27:53.00 AS.reconfigure ASfast @17:27:53.05 AS.seek 0ml @17:28:20.40 ARV.dial between carrier and air1 @17:28:20.88 ATV.dial :air3 @17:28:22.09 AS.reconfigure ASslow1 @17:28:22.14 AS.seek 0.01ml @17:28:24.44 delay 2 seconds @17:28:26.46 ATV.dial :bleach @17:28:27.11 AS.seek 0.81ml @17:31:08.23 delay 3 seconds @17:31:11.25 ATV.dial :air3 @17:31:11.79 delay 3 seconds @17:31:14.88 AS.seek 0.835ml @17:31:20.18 delay 3 seconds @17:31:23.20 ATV.dial :bleach @17:31:23.80 AS.seek 0.82ml @17:31:27.12 delay 3 seconds @17:31:30.14 ATV.dial PCR @17:31:30.65 AS.reconfigure ASconfig @17:31:30.70 AS.seek :empty @17:32:18.18 PCR.program :bleachProfile @17:32:18.24 PCR program should complete in < 2:45 @17:32:18.25 PCR.run 10:00 @17:35:29.16 PCR.off @17:35:29.18 AFV.select :carrierSolution @17:35:29.30 delay 2 seconds @17:35:31.33 AS.seek 1ml @17:36:29.18 delay 1 second @17:36:30.19 AFV.select :holdingCoil @17:36:30.30 delay 1 second @17:36:31.33 AS.reconfigure ASfast @17:36:31.38 AS.seek 0ml @17:36:58.73 delay 3 seconds @17:37:01.75 AFV.select :carrierSolution @17:37:01.86 delay 2 seconds @17:37:03.89 AS.reconfigure ASconfig @17:37:03.95 AS.seek 1ml @17:38:01.73 delay 1 second @17:38:02.74 AFV.select :holdingCoil @17:38:02.85 delay 1 second @17:38:03.88 AS.reconfigure ASfast @17:38:03.93 AS.seek 0ml @17:38:31.29 delay 3 seconds @17:38:34.31 AFV.select :carrierSolution @17:38:34.42 delay 2 seconds @17:38:36.45 AS.reconfigure ASconfig @17:38:36.51 AS.seek 1ml @17:39:34.29 delay 1 second @17:39:35.30 AFV.select :holdingCoil @17:39:35.41 delay 1 second @17:39:36.44 AS.reconfigure ASfast @17:39:36.49 AS.seek 0ml @17:40:03.84 delay 3 seconds @17:40:06.86 AFV.select :carrierSolution @17:40:06.97 delay 2 seconds @17:40:09.00 AS.reconfigure ASconfig @17:40:09.05 AS.seek 1ml @17:41:06.84 delay 1 second @17:41:07.85 AFV.select :holdingCoil @17:41:07.96 delay 1 second @17:41:08.99 AS.reconfigure ASfast @17:41:09.04 AS.seek 0ml @17:41:36.40 delay 3 seconds @17:41:39.42 AFV.select :carrierSolution @17:41:39.53 delay 2 seconds @17:41:41.56 AS.reconfigure ASconfig @17:41:41.61 AS.seek 1ml @17:42:39.39 delay 1 second @17:42:40.40 AFV.select :holdingCoil @17:42:40.51 delay 1 second @17:42:41.54 AS.reconfigure ASnorm @17:42:41.59 AS.seek 0ml @17:43:32.33 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1317h150ml.pcr @17:43:32.35 Building PCR Slug from ASV with MM2 mix, PP4 primer @17:43:32.36 Priming with carrier @17:43:32.37 ATV.dial ARV @17:43:33.06 ARV.dial :carrier @17:43:33.41 AS.seek 0.5ml @17:43:58.95 AFV.select :carrierSolution @17:43:59.07 delay 2 seconds @17:44:01.10 AS.reconfigure ASconfig @17:44:01.15 AS.seek 1ml @17:44:30.18 delay 1 second @17:44:31.19 AFV.select :holdingCoil @17:44:31.30 delay 1 second @17:44:32.32 ATV.dial :waste @17:44:33.87 AS.reconfigure ASnorm @17:44:33.92 AS.seek :empty @17:45:25.24 AFV.select :carrierSolution @17:45:25.36 delay 2 seconds @17:45:27.39 AS.reconfigure ASconfig @17:45:27.44 AS.seek 0.5ml @17:45:56.54 delay 1 second @17:45:57.56 AFV.select :holdingCoil @17:45:57.67 Building Slug @17:45:57.68 ATV.dial :ARV @17:45:59.20 delay 2 seconds @17:46:01.22 ARV.dial :air1 @17:46:01.62 delay 2 seconds @17:46:03.71 AS.reconfigure ASslow2 @17:46:03.76 AS.seek 0.506ml @17:46:10.03 delay 2 seconds @17:46:12.05 ARV.dial :MM2 @17:46:13.45 delay 2 seconds @17:46:15.48 Adding MM2 @17:46:15.56 AS.seek 0.524ml @17:46:33.81 delay 25 seconds @17:46:58.83 ARV.dial :PP4 @17:46:59.22 Adding PP4 @17:46:59.24 delay 2 seconds @17:47:01.32 AS.seek 0.53ml @17:47:07.56 delay 10 seconds @17:47:17.58 ARV.dial :air3 @17:47:17.97 adding ARV air @17:47:17.99 delay 2 seconds @17:47:20.07 AS.seek 0.536ml @17:47:26.29 delay 5 seconds @17:47:31.31 ARV.dial :carrier @17:47:32.51 delay 7 seconds @17:47:39.60 AS.seek 0.594ml @17:48:38.33 delay 5 seconds @17:48:43.35 ATV.dial :ASV @17:48:43.75 delay 2 seconds @17:48:45.84 AS.seek 0.6ml @17:48:52.01 delay 4 seconds @17:48:56.03 ATV.dial ARV @17:48:56.56 delay 2 seconds @17:48:58.58 ARV.dial :carrier @17:48:58.60 delay 2 seconds @17:49:00.69 AS.seek 0.65ml @17:49:51.11 delay 4 seconds @17:49:55.13 ATV.dial PCR @17:49:55.72 ASV.dial :waste1 @17:49:55.73 ARV.dial :air1 @17:49:56.12 Positioning Slug by 0.324ml @17:49:56.21 AS.seek 0.326ml @17:55:22.25 delay 5 seconds @17:55:27.27 ATV.dial ARV @17:55:28.11 ASV.dial :PCRlock @17:55:28.50 delay 5 seconds @17:55:33.53 Running Tt1 Profile60C300 thermocycling @17:55:33.54 PCR.program Profile60C300 @17:55:33.68 Profile60C300 should complete in < 1:45:21 @17:55:33.69 PCR.run 2:16:41.25 @19:17:29.70 PCR.off @19:17:31.27 PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1317h150ml.pcr @19:17:31.35 Cleaning PCR @19:17:31.36 AFV.select :carrierSolution @19:17:31.47 delay 2 seconds @19:17:33.50 AS.reconfigure ASconfig @19:17:33.55 AS.seek 0.5ml @19:17:43.86 delay 1 second @19:17:44.87 AFV.select :holdingCoil @19:17:44.98 ASV.dial :waste1 @19:17:45.37 ATV.dial :air3 @19:17:46.57 AS.reconfigure ASslow1 @19:17:46.63 AS.seek 0.51ml @19:17:48.92 delay 2 seconds @19:17:50.94 ATV.dial :bleach @19:17:51.54 AS.seek 0.81ml @19:18:52.14 delay 3 seconds @19:18:55.16 ATV.dial :air3 @19:18:55.69 delay 3 seconds @19:18:58.78 AS.seek 0.835ml @19:19:04.14 delay 3 seconds @19:19:07.16 ATV.dial :bleach @19:19:07.86 AS.seek 0.82ml @19:19:11.14 delay 3 seconds @19:19:14.16 ARV.dial :waste @19:19:14.75 delay 3 seconds @19:19:17.77 ATV.dial :ARV @19:19:18.60 AS.reconfigure ASconfig @19:19:18.66 AS.seek 0.47ml @19:19:39.07 AFV.select :carrierSolution @19:19:39.19 delay 2 seconds @19:19:41.82 AS.seek 1ml @19:20:12.56 delay 1 second @19:20:13.57 AFV.select :holdingCoil @19:20:13.68 delay 1 second @19:20:14.71 AS.reconfigure ASfast @19:20:14.76 AS.seek 0ml @19:20:42.10 AFV.select :carrierSolution @19:20:42.22 delay 2 seconds @19:20:44.25 AS.reconfigure ASconfig @19:20:44.30 AS.seek 1ml @19:21:42.09 delay 1 second @19:21:43.10 AFV.select :holdingCoil @19:21:43.21 delay 1 second @19:21:44.24 AS.reconfigure ASfast @19:21:44.29 AS.seek 0ml @19:22:11.63 AFV.select :carrierSolution @19:22:11.75 delay 2 seconds @19:22:13.78 AS.reconfigure ASconfig @19:22:13.83 AS.seek 1ml @19:23:11.66 delay 1 second @19:23:12.67 AFV.select :holdingCoil @19:23:12.78 delay 1 second @19:23:13.81 AS.reconfigure ASfast @19:23:13.86 AS.seek 0ml @19:23:41.19 ARV.dial between carrier and air1 @19:23:41.69 ATV.dial :air3 @19:23:42.90 AS.reconfigure ASslow1 @19:23:42.95 AS.seek 0.01ml @19:23:45.28 delay 2 seconds @19:23:47.29 ATV.dial :bleach @19:23:47.91 AS.seek 0.81ml @19:26:29.05 delay 3 seconds @19:26:32.07 ATV.dial :air3 @19:26:32.59 delay 3 seconds @19:26:35.68 AS.seek 0.835ml @19:26:40.98 delay 3 seconds @19:26:44.00 ATV.dial :bleach @19:26:44.60 AS.seek 0.82ml @19:26:48.00 delay 3 seconds @19:26:51.02 ATV.dial PCR @19:26:51.41 AS.reconfigure ASconfig @19:26:51.46 AS.seek :empty @19:27:38.90 PCR.program :bleachProfile @19:27:38.97 PCR program should complete in < 2:45 @19:27:38.98 PCR.run 10:00 @19:30:49.88 PCR.off @19:30:49.91 AFV.select :carrierSolution @19:30:50.03 delay 2 seconds @19:30:52.06 AS.seek 1ml @19:31:49.88 delay 1 second @19:31:50.89 AFV.select :holdingCoil @19:31:51.00 delay 1 second @19:31:52.03 AS.reconfigure ASfast @19:31:52.08 AS.seek 0ml @19:32:19.42 delay 3 seconds @19:32:22.44 AFV.select :carrierSolution @19:32:22.55 delay 2 seconds @19:32:24.58 AS.reconfigure ASconfig @19:32:24.65 AS.seek 1ml @19:33:22.42 delay 1 second @19:33:23.43 AFV.select :holdingCoil @19:33:23.54 delay 1 second @19:33:24.57 AS.reconfigure ASfast @19:33:24.62 AS.seek 0ml @19:33:51.96 delay 3 seconds @19:33:54.98 AFV.select :carrierSolution @19:33:55.09 delay 2 seconds @19:33:57.12 AS.reconfigure ASconfig @19:33:57.18 AS.seek 1ml @19:34:54.96 delay 1 second @19:34:55.97 AFV.select :holdingCoil @19:34:56.08 delay 1 second @19:34:57.11 AS.reconfigure ASfast @19:34:57.16 AS.seek 0ml @19:35:24.50 delay 3 seconds @19:35:27.52 AFV.select :carrierSolution @19:35:27.63 delay 2 seconds @19:35:29.66 AS.reconfigure ASconfig @19:35:29.72 AS.seek 1ml @19:36:27.52 delay 1 second @19:36:28.53 AFV.select :holdingCoil @19:36:28.64 delay 1 second @19:36:29.67 AS.reconfigure ASfast @19:36:29.72 AS.seek 0ml @19:36:57.16 delay 3 seconds @19:37:00.18 AFV.select :carrierSolution @19:37:00.29 delay 2 seconds @19:37:02.32 AS.reconfigure ASconfig @19:37:02.38 AS.seek 1ml @19:38:00.22 delay 1 second @19:38:01.23 AFV.select :holdingCoil @19:38:01.34 delay 1 second @19:38:02.37 AS.reconfigure ASnorm @19:38:02.42 AS.seek 0ml @19:38:53.11 Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1319h150ml.pcr @19:38:53.13 Building PCR Slug from ASV with MM1 mix, PP1 primer @19:38:53.15 Priming with carrier @19:38:53.16 ATV.dial ARV @19:38:53.83 ARV.dial :carrier @19:38:54.17 AS.seek 0.5ml @19:39:20.28 AFV.select :carrierSolution @19:39:20.40 delay 2 seconds @19:39:22.43 AS.reconfigure ASconfig @19:39:22.48 AS.seek 1ml @19:39:51.52 delay 1 second @19:39:52.54 AFV.select :holdingCoil @19:39:52.65 delay 1 second @19:39:53.68 ATV.dial :waste @19:39:55.32 AS.reconfigure ASnorm @19:39:55.37 AS.seek :empty @19:40:46.12 AFV.select :carrierSolution @19:40:46.24 delay 2 seconds @19:40:48.27 AS.reconfigure ASconfig @19:40:48.32 AS.seek 0.5ml @19:41:17.43 delay 1 second @19:41:18.44 AFV.select :holdingCoil @19:41:18.55 Building Slug @19:41:18.56 ATV.dial :ARV @19:41:20.05 delay 2 seconds @19:41:22.07 ARV.dial :air1 @19:41:22.56 delay 2 seconds @19:41:24.65 AS.reconfigure ASslow2 @19:41:24.71 AS.seek 0.506ml @19:41:31.05 delay 2 seconds @19:41:33.07 ARV.dial :MM1 @19:41:33.66 delay 2 seconds @19:41:35.69 Adding MM1 @19:41:35.76 AS.seek 0.524ml @19:41:54.27 delay 25 seconds @19:42:19.29 ARV.dial :PP1 @19:42:19.68 Adding PP1 @19:42:19.69 delay 2 seconds @19:42:21.78 AS.seek 0.53ml @19:42:28.11 delay 10 seconds @19:42:38.13 ARV.dial :air1 @19:42:38.51 adding ARV air @19:42:38.52 delay 2 seconds @19:42:40.61 AS.seek 0.536ml @19:42:46.83 delay 5 seconds @19:42:51.85 ARV.dial :carrier @19:42:52.26 delay 7 seconds @19:42:59.35 AS.seek 0.594ml @19:43:57.79 delay 5 seconds @19:44:02.81 ATV.dial :ASV @19:44:03.21 delay 2 seconds @19:44:05.30 AS.seek 0.6ml @19:44:11.65 delay 4 seconds @19:44:15.67 ATV.dial ARV @19:44:16.21 delay 2 seconds @19:44:18.23 ARV.dial :carrier @19:44:18.25 delay 2 seconds @19:44:20.34 AS.seek 0.65ml @19:45:10.82 delay 4 seconds @19:45:14.84 ATV.dial PCR @19:45:15.41 ASV.dial :waste1 @19:45:15.42 ARV.dial :air1 @19:45:15.80 Positioning Slug by 0.324ml @19:45:15.90 AS.seek 0.326ml @19:50:42.12 delay 5 seconds @19:50:47.14 ATV.dial ARV @19:50:47.98 ASV.dial :PCRlock @19:50:48.37 delay 5 seconds @19:50:53.40 Running IPc ProfileIPc thermocycling @19:50:53.41 PCR.program ProfileIPc @19:50:53.57 ProfileIPc should complete in < 1:50:49 @19:50:53.58 PCR.run 2:23:31.25 @21:15:09.33 PCR.off @21:15:12.45 PCR for IPc completed -- closed /var/log/esp/IPc18oct1319h150ml.pcr @21:15:12.47 Full MFB Decontamination @21:15:12.48 Decon extraction column and decon AIV @21:15:12.50 SPE.hold 2:46:40,55C @21:15:12.56 ASV.dial :SPEdelivery @21:15:13.19 delay 20 seconds @21:15:33.21 AFV.select :carrierSolution @21:15:33.32 delay 2 seconds @21:15:35.35 AS.reconfigure ASconfig @21:15:35.40 AS.seek 0.5ml @21:15:45.69 delay 1 second @21:15:46.70 AFV.select :holdingCoil @21:15:46.81 ATV.dial :air1 @21:15:48.59 AS.reconfigure ASslow1 @21:15:48.64 AS.seek 0.52ml @21:15:52.93 ATV.dial :ASV @21:15:54.58 ASV.dial :SPErecovery @21:15:55.22 AS.seek 0.62ml @21:16:15.62 ATV.dial :waste @21:16:17.47 AS.reconfigure ASnorm @21:16:17.52 AS.seek :empty @21:16:49.15 AFV.select :holdingCoil @21:16:49.17 ATV.dial :waste @21:16:49.19 AS.reconfigure ASconfig @21:16:49.24 AS.seek 0ml @21:16:49.56 AFV.select :carrierSolution @21:16:49.68 delay 2 seconds @21:16:51.71 AS.seek 0.5ml @21:17:20.74 delay 1 second @21:17:21.75 AFV.select :holdingCoil @21:17:21.86 ATV.dial :air3 @21:17:22.54 AS.reconfigure ASslow1 @21:17:22.59 AS.seek 0.52ml @21:17:26.89 ATV.dial :air3 @21:17:26.98 AS.seek 0.53ml @21:17:29.27 delay 2 seconds @21:17:31.28 ATV.dial :bleach @21:17:31.76 AS.seek 0.71ml @21:18:08.18 delay 3 seconds @21:18:11.20 ATV.dial :air3 @21:18:11.74 delay 3 seconds @21:18:14.83 AS.seek 0.735ml @21:18:20.13 delay 3 seconds @21:18:23.15 ATV.dial :bleach @21:18:23.76 AS.seek 0.72ml @21:18:27.07 delay 3 seconds @21:18:30.10 ATV.dial ASV @21:18:31.09 ASV.dial :SPErecovery @21:18:31.18 AS.seek 0.365ml @21:19:42.78 ATV.dial :waste @21:19:44.51 AS.reconfigure ASnorm @21:19:44.56 AS.seek :empty @21:20:03.33 ATV.dial :air3 @21:20:04.11 AS.reconfigure ASslow1 @21:20:04.16 AS.seek 0.009ml @21:20:06.51 delay 2 seconds @21:20:08.52 ATV.dial :bleach @21:20:09.01 AS.seek 0.579ml @21:22:03.88 delay 3 seconds @21:22:06.90 ATV.dial :air3 @21:22:07.45 delay 3 seconds @21:22:10.54 AS.seek 0.604ml @21:22:15.85 delay 3 seconds @21:22:18.87 ATV.dial :bleach @21:22:19.47 AS.seek 0.589ml @21:22:22.77 delay 3 seconds @21:22:25.79 ATV.dial :ASV @21:22:26.77 ASV.dial :wasteR @21:22:27.23 AS.seek 0ml @21:24:26.01 delay 1:00 @21:25:26.03 ATV.dial ASV @21:25:26.05 AFV.select :carrierSolution @21:25:26.15 delay 2 seconds @21:25:28.18 AS.reconfigure ASconfig @21:25:28.23 AS.seek 1ml @21:26:25.98 delay 1 second @21:26:26.99 AFV.select :holdingCoil @21:26:27.10 delay 1 second @21:26:28.12 ASV.dial :wasteR @21:26:28.73 AS.reconfigure ASslow1 @21:26:28.79 AS.seek 0ml @21:29:50.17 delay 10 seconds @21:30:00.20 ATV.dial :air3 @21:30:01.64 AS.seek 0.01ml @21:30:03.85 delay 2 seconds @21:30:05.86 ATV.dial :bleach @21:30:06.49 AS.seek 0.58ml @21:32:01.40 delay 3 seconds @21:32:04.42 ATV.dial :air3 @21:32:04.96 delay 3 seconds @21:32:08.05 AS.seek 0.605ml @21:32:13.35 delay 3 seconds @21:32:16.37 ATV.dial :bleach @21:32:17.08 AS.seek 0.59ml @21:32:20.42 delay 3 seconds @21:32:23.45 ATV.dial ASV @21:32:24.43 ASV.dial :SPEdelivery @21:32:24.80 AS.seek 0ml @21:34:23.70 delay 1:00 @21:35:23.72 AFV.select :carrierSolution @21:35:23.83 delay 2 seconds @21:35:25.86 AS.reconfigure ASconfig @21:35:25.91 AS.seek 1ml @21:36:23.65 delay 1 second @21:36:24.66 AFV.select :holdingCoil @21:36:24.77 delay 1 second @21:36:25.79 ATV.dial ASV @21:36:25.81 ASV.dial :SPEdelivery @21:36:25.83 AS.reconfigure ASslow1 @21:36:25.88 AS.seek 0ml @21:39:47.31 delay 10 seconds @21:39:57.33 ATV.dial :air2 @21:39:58.12 AS.reconfigure ASslow0 @21:39:58.18 AS.seek 0.8ml @21:41:18.97 delay 3 seconds @21:41:21.99 ATV.dial :ASV @21:41:22.93 ASV.dial :SPEdelivery @21:41:22.96 AS.reconfigure ASslow1 @21:41:23.01 AS.seek 0.03ml @21:43:58.15 delay 20 seconds @21:44:18.18 ATV.dial :waste,via: :air3 @21:44:19.89 AS.reconfigure ASconfig @21:44:19.94 AS.seek :empty @21:44:22.00 SPE.coast @21:44:22.09 Removing Bleach from Mixing Coil @21:44:22.10 AFV.select :carrierSolution @21:44:22.21 delay 2 seconds @21:44:24.24 AS.seek 0.5ml @21:44:53.34 delay 1 second @21:44:54.35 AFV.select :holdingCoil @21:44:54.46 ATV.dial :ASV @21:44:56.06 ASV.dial :SPErecovery @21:44:56.69 AS.reconfigure ASslow1 @21:44:56.74 AS.seek 0.73ml @21:45:43.29 ASV.dial :wasteD @21:45:43.94 AS.reconfigure ASnorm @21:45:44.00 AS.seek 0ml @21:46:21.13 AFV.select :carrierSolution @21:46:21.25 delay 2 seconds @21:46:23.28 AS.reconfigure ASconfig @21:46:23.33 AS.seek 0.5ml @21:46:52.39 delay 1 second @21:46:53.40 AFV.select :holdingCoil @21:46:53.51 ATV.dial ASV @21:46:53.52 ASV.dial :SPErecovery @21:46:54.15 AS.reconfigure ASslow0 @21:46:54.20 AS.seek 0.3ml @21:47:14.61 ATV.dial :air2 @21:47:15.60 AS.reconfigure ASslow1 @21:47:15.65 AS.seek 0.32ml @21:47:19.90 ATV.dial ASV @21:47:20.98 AS.reconfigure ASslow0 @21:47:21.03 AS.seek 0.57ml @21:47:46.49 ASV.dial :wasteD @21:47:47.07 AS.reconfigure ASnorm @21:47:47.12 AS.seek 0ml @21:48:16.21 AFV.select :carrierSolution @21:48:16.33 delay 2 seconds @21:48:18.36 AS.reconfigure ASconfig @21:48:18.42 AS.seek 0.5ml @21:48:47.45 delay 1 second @21:48:48.46 AFV.select :holdingCoil @21:48:48.57 ATV.dial ASV @21:48:48.58 ASV.dial :SPErecovery @21:48:49.21 AS.reconfigure ASslow0 @21:48:49.26 AS.seek 0.3ml @21:49:09.72 ATV.dial :air2 @21:49:10.69 AS.reconfigure ASslow1 @21:49:10.74 AS.seek 0.32ml @21:49:15.63 ATV.dial ASV @21:49:16.63 AS.reconfigure ASslow0 @21:49:16.68 AS.seek 0.57ml @21:49:42.16 ASV.dial :wasteD @21:49:42.73 AS.reconfigure ASnorm @21:49:42.79 AS.seek 0ml @21:50:11.85 ATV.dial :air2 @21:50:12.78 AS.reconfigure ASslow0 @21:50:12.84 AS.seek 0.3ml @21:50:43.37 ATV.dial :ASV @21:50:44.30 ASV.dial :wasteD @21:50:44.32 AS.reconfigure ASconfig @21:50:44.37 AS.seek 0.05ml @21:50:59.07 ATV.dial :waste @21:51:00.91 AS.seek 0ml @21:51:04.13 ASV.dial :SPEdelivery @21:51:05.08 deconLysateINT @21:51:05.09 ATV.dial :air3 @21:51:05.76 AS.reconfigure ASslow1 @21:51:05.81 AS.seek 0.01ml @21:51:08.16 delay 2 seconds @21:51:10.17 ATV.dial :bleach @21:51:10.73 AS.seek 0.96ml @21:54:22.03 delay 3 seconds @21:54:25.05 ATV.dial :air3 @21:54:25.58 delay 3 seconds @21:54:28.67 AS.seek 0.985ml @21:54:33.99 delay 3 seconds @21:54:37.01 ATV.dial :bleach @21:54:37.62 AS.seek 0.97ml @21:54:40.94 delay 3 seconds @21:54:43.97 ATV.dial AIV @21:54:45.13 AIV.dial :coilToMFB @21:54:45.14 AWV.select :waste @21:54:45.16 AFV.select :carrierSolution @21:54:45.27 delay 2 seconds @21:54:47.30 AS.reconfigure ASconfig @21:54:47.35 AS.seek 1ml @21:54:49.38 delay 1 second @21:54:50.39 AFV.select :holdingCoil @21:54:50.50 delay 1 second @21:54:51.53 AS.reconfigure ASslow0 @21:54:51.58 AS.seek :empty @21:56:32.52 AFV.select :carrierSolution @21:56:32.64 delay 2 seconds @21:56:34.67 AS.reconfigure ASconfig @21:56:34.72 AS.seek 1ml @21:57:32.52 delay 1 second @21:57:33.53 AFV.select :holdingCoil @21:57:33.64 delay 1 second @21:57:34.67 AS.reconfigure ASslow0 @21:57:34.72 AS.seek :empty @21:59:15.65 AFV.select :carrierSolution @21:59:15.77 delay 2 seconds @21:59:17.80 AS.reconfigure ASconfig @21:59:17.85 AS.seek 1ml @22:00:15.69 delay 1 second @22:00:16.70 AFV.select :holdingCoil @22:00:16.81 delay 1 second @22:00:17.84 AS.reconfigure ASslow0 @22:00:17.88 AS.seek :empty @22:01:58.82 ATV.dial :air2 @22:01:59.46 AS.reconfigure ASslow1 @22:01:59.51 AS.seek 0.009ml @22:02:01.80 AS.reconfigure ASslow0 @22:02:01.86 AS.seek 1ml @22:03:41.79 ATV.dial AIV @22:03:42.55 AS.seek 0.02ml @22:05:21.43 ATV.dial :waste @22:05:22.96 AS.seek 0ml @22:05:25.24 AWV.select :air @22:05:25.41 Cleaning PCR @22:05:25.42 AFV.select :carrierSolution @22:05:25.53 delay 2 seconds @22:05:27.56 AS.reconfigure ASconfig @22:05:27.61 AS.seek 0.5ml @22:05:56.64 delay 1 second @22:05:57.65 AFV.select :holdingCoil @22:05:57.76 ASV.dial :waste1 @22:05:58.13 ATV.dial :air3 @22:05:58.79 AS.reconfigure ASslow1 @22:05:58.84 AS.seek 0.51ml @22:06:01.13 delay 2 seconds @22:06:03.14 ATV.dial :bleach @22:06:03.70 AS.seek 0.81ml @22:07:04.30 delay 3 seconds @22:07:07.32 ATV.dial :air3 @22:07:07.84 delay 3 seconds @22:07:10.93 AS.seek 0.835ml @22:07:16.81 delay 3 seconds @22:07:19.83 ATV.dial :bleach @22:07:20.43 AS.seek 0.82ml @22:07:23.74 delay 3 seconds @22:07:26.77 ARV.dial :waste @22:07:27.35 delay 3 seconds @22:07:30.37 ATV.dial :ARV @22:07:31.22 AS.reconfigure ASconfig @22:07:31.27 AS.seek 0.47ml @22:07:51.68 AFV.select :carrierSolution @22:07:51.80 delay 2 seconds @22:07:53.83 AS.seek 1ml @22:08:24.58 delay 1 second @22:08:25.59 AFV.select :holdingCoil @22:08:25.70 delay 1 second @22:08:26.73 AS.reconfigure ASfast @22:08:26.78 AS.seek 0ml @22:08:54.23 AFV.select :carrierSolution @22:08:54.35 delay 2 seconds @22:08:56.38 AS.reconfigure ASconfig @22:08:56.43 AS.seek 1ml @22:09:54.22 delay 1 second @22:09:55.23 AFV.select :holdingCoil @22:09:55.34 delay 1 second @22:09:56.37 AS.reconfigure ASfast @22:09:56.42 AS.seek 0ml @22:10:23.75 AFV.select :carrierSolution @22:10:23.87 delay 2 seconds @22:10:25.90 AS.reconfigure ASconfig @22:10:25.95 AS.seek 1ml @22:11:23.72 delay 1 second @22:11:24.74 AFV.select :holdingCoil @22:11:24.85 delay 1 second @22:11:25.88 AS.reconfigure ASfast @22:11:25.93 AS.seek 0ml @22:11:53.26 ARV.dial between carrier and air1 @22:11:53.76 ATV.dial :air3 @22:11:54.97 AS.reconfigure ASslow1 @22:11:55.02 AS.seek 0.01ml @22:11:57.38 delay 2 seconds @22:11:59.39 ATV.dial :bleach @22:12:00.00 AS.seek 0.81ml @22:14:41.15 delay 3 seconds @22:14:44.17 ATV.dial :air3 @22:14:44.71 delay 3 seconds @22:14:47.80 AS.seek 0.835ml @22:14:53.12 delay 3 seconds @22:14:56.14 ATV.dial :bleach @22:14:56.75 AS.seek 0.82ml @22:15:00.05 delay 3 seconds @22:15:03.07 ATV.dial PCR @22:15:03.46 AS.reconfigure ASconfig @22:15:03.51 AS.seek :empty @22:15:50.98 PCR.program :bleachProfile @22:15:51.05 PCR program should complete in < 2:45 @22:15:51.06 PCR.run 10:00 @22:19:11.06 PCR.off @22:19:11.08 AFV.select :carrierSolution @22:19:11.20 delay 2 seconds @22:19:13.23 AS.seek 1ml @22:20:11.06 delay 1 second @22:20:12.07 AFV.select :holdingCoil @22:20:12.18 delay 1 second @22:20:13.21 AS.reconfigure ASfast @22:20:13.26 AS.seek 0ml @22:20:40.62 delay 3 seconds @22:20:43.64 AFV.select :carrierSolution @22:20:43.75 delay 2 seconds @22:20:45.78 AS.reconfigure ASconfig @22:20:45.84 AS.seek 1ml @22:21:43.63 delay 1 second @22:21:44.64 AFV.select :holdingCoil @22:21:44.75 delay 1 second @22:21:45.78 AS.reconfigure ASfast @22:21:45.83 AS.seek 0ml @22:22:13.18 delay 3 seconds @22:22:16.20 AFV.select :carrierSolution @22:22:16.31 delay 2 seconds @22:22:18.34 AS.reconfigure ASconfig @22:22:18.40 AS.seek 1ml @22:23:16.18 delay 1 second @22:23:17.19 AFV.select :holdingCoil @22:23:17.30 delay 1 second @22:23:18.33 AS.reconfigure ASfast @22:23:18.39 AS.seek 0ml @22:23:45.73 delay 3 seconds @22:23:48.75 AFV.select :carrierSolution @22:23:48.86 delay 2 seconds @22:23:50.89 AS.reconfigure ASconfig @22:23:50.95 AS.seek 1ml @22:24:48.74 delay 1 second @22:24:49.75 AFV.select :holdingCoil @22:24:49.86 delay 1 second @22:24:50.89 AS.reconfigure ASfast @22:24:50.94 AS.seek 0ml @22:25:18.89 delay 3 seconds @22:25:21.91 AFV.select :carrierSolution @22:25:22.02 delay 2 seconds @22:25:24.05 AS.reconfigure ASconfig @22:25:24.11 AS.seek 1ml @22:26:21.89 delay 1 second @22:26:22.90 AFV.select :holdingCoil @22:26:23.01 delay 1 second @22:26:24.04 AS.reconfigure ASnorm @22:26:24.09 AS.seek 0ml @22:27:14.79 AFV.select :carrierSolution @22:27:14.90 delay 2 seconds @22:27:16.93 AS.reconfigure ASconfig @22:27:16.98 AS.seek 1ml @22:28:14.76 delay 1 second @22:28:15.77 AFV.select :holdingCoil @22:28:15.88 delay 1 second @22:28:16.90 ATV.dial :waste @22:28:18.04 AS.reconfigure ASnorm @22:28:18.10 AS.seek 0.5ml @22:28:43.64 ATV.dial :waste2 @22:28:44.84 AS.seek 0ml @22:29:10.36 AS.coast @22:29:10.41 SS.coast @22:29:10.45 CC.coast @22:29:10.49 CS.coast @22:29:10.53 PC.coast @22:29:10.57 PS.coast @22:29:10.61 Forearm.coast @22:29:10.66 Elbow.coast @22:29:11.05 Gate.power :core,:OFF @22:29:11.07 Gate.power :raw,:OFF @22:29:11.08 Shutdown @22:29:11.09 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @09:45:04.39CEST15-Oct-18 Session Begun @09:45:06.75 -> can @09:45:16.83 -> exit @09:45:16.85 Session Ended @11:38:26.82 Session Begun @11:38:31.84 -> ESP.wakeup @11:38:31.92 Powering core bus @11:38:36.65 Gate.power :raw,:ON @11:38:37.35 SPE.coast @11:38:37.45 SPE.configure SPEconfig @11:38:37.57 AS.configure ASconfig @11:38:37.69 SS.configure SSconfig @11:38:37.84 CC.configure CCconfig @11:38:38.01 CS.configure CSconfig @11:38:38.14 PC.configure PCconfig @11:38:38.27 PS.configure PSconfig @11:38:38.41 Forearm.configure LooseForearm @11:38:38.54 Elbow.configure LooseElbow @11:38:38.69 SC.configure SCloose @11:38:38.82 SE.configure SEconfig @11:38:38.89 CH.coast @11:38:38.97 CH.configure CHconfig @11:38:39.03 PH.coast @11:38:39.12 PH.configure PHconfig @11:38:39.19 PCR.off @11:38:39.25 PCR.pidtemp= 50,0.1,0,300,25,25 @11:38:39.29 PCR.caltemp= 2.7073,0.0373,0 @11:38:39.33 All dwarf controllers running firmware version 3.75 @11:38:39.47 Valve.reset @11:38:39.67 PCR.off @11:38:39.72 PCR.pidtemp= 50,0.1,0,300,25,25 @11:38:39.77 PCR.caltemp= 2.7073,0.0373,0 @11:38:39.88 Intake.select :close @11:38:40.00 Exhaust.select :close @11:38:40.02 CTV.dial :air @11:38:40.15 PSV.dial :air @11:38:40.18 CSV.dial :waste1 @11:38:40.39 SSV.dial :spit,avoiding: :exhaust @11:38:40.41 Forearm.reconfigure LooseForearm @11:38:40.56 Forearm.home.seek :retract @11:38:40.78 PS.reconfigure PSconfig @11:38:40.85 PS.home.seek 0ml @11:38:41.34 Hand.close @11:38:41.38 Elbow.jog 1500 @11:38:41.55 CS.reconfigure CSfast @11:38:41.58 SS.reconfigure SSconfig @11:38:41.70 CS.home.seek 0ml @11:38:41.74 SS.home.seek 0ml @11:38:42.04 Elbow.reconfigure LooseElbow @11:38:42.14 Elbow.home.seek :clear @11:38:45.57 SE.reconfigure SEconfig @11:38:45.64 SE.home.seek :down @11:38:46.36 SC.reconfigure SCsloppy @11:38:46.44 SC.home.seek :initial @11:38:49.93 PS.coast @11:38:50.44 Forearm.seek :retract @11:38:50.56 Elbow.seek :hammer @11:38:51.81 Forearm.reconfigure TightForearm @11:38:51.89 Forearm.seek 5 + 350 counts @11:38:52.96 Forearm.seek :retract @11:38:54.16 PC.reconfigure PCconfig @11:38:54.18 CC.reconfigure CCconfig @11:38:54.29 PC.home.seek :open @11:38:54.37 CC.home.seek :open @11:39:32.78 -> MFB.ready? @11:39:39.54 -> MFB.ready! @11:39:39.65 Readying the MFB @11:39:39.66 Homing MFB Syringe @11:39:39.69 AFV.select :holdingCoil @11:39:39.87 ATV.dial :air2 @11:39:40.44 AS.reconfigure ASconfig @11:39:40.52 AS.home.seek :home @11:39:46.60 AFV.select :carrierSolution @11:39:46.74 delay 2 seconds @11:39:48.77 AS.seek 1ml @11:40:41.70 delay 1 second @11:40:42.72 AFV.select :holdingCoil @11:40:42.86 delay 1 second @11:40:43.89 ATV.dial :waste @11:40:44.90 AS.reconfigure ASnorm @11:40:44.98 AS.home.seek :empty @11:41:37.58 ATV.dial :waste @11:41:37.60 ARV.dial between carrier and air1 @11:41:37.73 ASV.dial :waste1 @11:42:43.83 -> pcrDecon @11:42:43.93 Cleaning PCR @11:42:43.95 AFV.select :carrierSolution @11:42:44.09 delay 2 seconds @11:42:46.12 AS.reconfigure ASconfig @11:42:46.20 AS.seek 0.5ml @11:43:15.33 delay 1 second @11:43:16.34 AFV.select :holdingCoil @11:43:16.48 ASV.dial :waste1 @11:43:16.50 ATV.dial :air3 @11:43:17.19 AS.reconfigure ASslow1 @11:43:17.26 AS.seek 0.51ml @11:43:19.58 delay 2 seconds @11:43:21.59 ATV.dial :bleach @11:43:22.05 AS.seek 0.81ml @11:44:22.67 delay 3 seconds @11:44:25.69 ATV.dial :air3 @11:44:26.23 delay 3 seconds @11:44:29.32 AS.seek 0.835ml @11:44:34.71 delay 3 seconds @11:44:37.73 ATV.dial :bleach @11:44:38.35 AS.seek 0.82ml @11:44:41.62 delay 3 seconds @11:44:44.64 ARV.dial :waste @11:44:45.14 delay 3 seconds @11:44:48.16 ATV.dial :ARV @11:44:49.03 AS.reconfigure ASconfig @11:44:49.11 AS.seek 0.47ml @11:45:09.54 AFV.select :carrierSolution @11:45:09.68 delay 2 seconds @11:45:11.71 AS.seek 1ml @11:45:42.48 delay 1 second @11:45:43.49 AFV.select :holdingCoil @11:45:43.62 delay 1 second @11:45:44.65 AS.reconfigure ASfast @11:45:44.72 AS.seek 0ml @11:46:12.08 AFV.select :carrierSolution @11:46:12.22 delay 2 seconds @11:46:14.25 AS.reconfigure ASconfig @11:46:14.32 AS.seek 1ml @11:47:12.11 delay 1 second @11:47:13.12 AFV.select :holdingCoil @11:47:13.26 delay 1 second @11:47:14.29 AS.reconfigure ASfast @11:47:14.37 AS.seek 0ml @11:47:41.72 AFV.select :carrierSolution @11:47:41.86 delay 2 seconds @11:47:43.89 AS.reconfigure ASconfig @11:47:43.96 AS.seek 1ml @11:48:41.77 delay 1 second @11:48:42.78 AFV.select :holdingCoil @11:48:42.91 delay 1 second @11:48:43.94 AS.reconfigure ASfast @11:48:44.01 AS.seek 0ml @11:49:11.37 ARV.dial between carrier and air1 @11:49:11.88 ATV.dial :air3 @11:49:13.10 AS.reconfigure ASslow1 @11:49:13.17 AS.seek 0.01ml @11:49:15.55 delay 2 seconds @11:49:17.56 ATV.dial :bleach @11:49:18.20 AS.seek 0.81ml @11:51:59.36 delay 3 seconds @11:52:02.38 ATV.dial :air3 @11:52:02.92 delay 3 seconds @11:52:06.01 AS.seek 0.835ml @11:52:11.32 delay 3 seconds @11:52:14.34 ATV.dial :bleach @11:52:14.96 AS.seek 0.82ml @11:52:18.28 delay 3 seconds @11:52:21.30 ATV.dial PCR @11:52:21.71 AS.reconfigure ASconfig @11:52:21.78 AS.seek :empty @11:53:09.27 PCR.program :bleachProfile @11:53:09.35 PCR program should complete in < 2:45 @11:53:09.36 PCR.run 10:00 @11:56:44.54 PCR.off @11:56:44.58 AFV.select :carrierSolution @11:56:44.71 delay 2 seconds @11:56:46.74 AS.seek 1ml @11:57:44.58 delay 1 second @11:57:45.60 AFV.select :holdingCoil @11:57:45.73 delay 1 second @11:57:46.76 AS.reconfigure ASfast @11:57:46.83 AS.seek 0ml @11:58:14.19 delay 3 seconds @11:58:17.21 AFV.select :carrierSolution @11:58:17.35 delay 2 seconds @11:58:19.38 AS.reconfigure ASconfig @11:58:19.46 AS.seek 1ml @11:59:17.27 delay 1 second @11:59:18.28 AFV.select :holdingCoil @11:59:18.42 delay 1 second @11:59:19.45 AS.reconfigure ASfast @11:59:20.06 AS.seek 0ml @11:59:47.44 delay 3 seconds @11:59:50.46 AFV.select :carrierSolution @11:59:50.60 delay 2 seconds @11:59:52.63 AS.reconfigure ASconfig @11:59:52.71 AS.seek 1ml @12:00:50.52 delay 1 second @12:00:51.53 AFV.select :holdingCoil @12:00:51.66 delay 1 second @12:00:52.69 AS.reconfigure ASfast @12:00:52.76 AS.seek 0ml @12:01:20.13 delay 3 seconds @12:01:23.15 AFV.select :carrierSolution @12:01:23.29 delay 2 seconds @12:01:25.32 AS.reconfigure ASconfig @12:01:25.41 AS.seek 1ml @12:02:23.24 delay 1 second @12:02:24.25 AFV.select :holdingCoil @12:02:24.38 delay 1 second @12:02:25.41 AS.reconfigure ASfast @12:02:25.48 AS.seek 0ml @12:02:52.85 delay 3 seconds @12:02:55.87 AFV.select :carrierSolution @12:02:56.00 delay 2 seconds @12:02:58.03 AS.reconfigure ASconfig @12:02:58.10 AS.seek 1ml @12:03:55.91 delay 1 second @12:03:56.93 AFV.select :holdingCoil @12:03:57.06 delay 1 second @12:03:58.09 AS.reconfigure ASnorm @12:03:58.16 AS.seek 0ml @12:08:55.16 -> pcrDecon @12:08:55.27 Cleaning PCR @12:08:55.28 AFV.select :carrierSolution @12:08:55.42 delay 2 seconds @12:08:57.45 AS.reconfigure ASconfig @12:08:57.52 AS.seek 0.5ml @12:09:26.62 delay 1 second @12:09:27.63 AFV.select :holdingCoil @12:09:27.77 ASV.dial :waste1 @12:09:27.79 ATV.dial :air3 @12:09:28.62 AS.reconfigure ASslow1 @12:09:28.69 AS.seek 0.51ml @12:09:30.99 delay 2 seconds @12:09:33.02 ATV.dial :bleach @12:09:33.62 AS.seek 0.81ml @12:10:34.24 delay 3 seconds @12:10:37.26 ATV.dial :air3 @12:10:37.81 delay 3 seconds @12:10:40.90 AS.seek 0.835ml @12:10:46.21 delay 3 seconds @12:10:49.23 ATV.dial :bleach @12:10:49.84 AS.seek 0.82ml @12:10:53.15 delay 3 seconds @12:10:56.17 ARV.dial :waste @12:10:56.66 delay 3 seconds @12:10:59.68 ATV.dial :ARV @12:11:00.65 AS.reconfigure ASconfig @12:11:00.73 AS.seek 0.47ml @12:11:21.14 AFV.select :carrierSolution @12:11:21.29 delay 2 seconds @12:11:23.32 AS.seek 1ml @12:11:54.15 delay 1 second @12:11:55.16 AFV.select :holdingCoil @12:11:55.29 delay 1 second @12:11:56.32 AS.reconfigure ASfast @12:11:56.39 AS.seek 0ml @12:12:23.75 AFV.select :carrierSolution @12:12:23.89 delay 2 seconds @12:12:25.92 AS.reconfigure ASconfig @12:12:25.99 AS.seek 1ml @12:13:23.81 delay 1 second @12:13:24.82 AFV.select :holdingCoil @12:13:24.96 delay 1 second @12:13:25.99 AS.reconfigure ASfast @12:13:26.07 AS.seek 0ml @12:13:53.44 AFV.select :carrierSolution @12:13:53.59 delay 2 seconds @12:13:55.62 AS.reconfigure ASconfig @12:13:55.70 AS.seek 1ml @12:14:53.46 delay 1 second @12:14:54.47 AFV.select :holdingCoil @12:14:54.60 delay 1 second @12:14:55.63 AS.reconfigure ASfast @12:14:55.70 AS.seek 0ml @12:15:23.14 ARV.dial between carrier and air1 @12:15:23.65 ATV.dial :air3 @12:15:24.88 AS.reconfigure ASslow1 @12:15:24.95 AS.seek 0.01ml @12:15:27.32 delay 2 seconds @12:15:29.34 ATV.dial :bleach @12:15:29.97 AS.seek 0.81ml @12:18:11.15 delay 3 seconds @12:18:14.17 ATV.dial :air3 @12:18:14.71 delay 3 seconds @12:18:17.80 AS.seek 0.835ml @12:18:23.12 delay 3 seconds @12:18:26.14 ATV.dial :bleach @12:18:26.75 AS.seek 0.82ml @12:18:30.07 delay 3 seconds @12:18:33.09 ATV.dial PCR @12:18:33.50 AS.reconfigure ASconfig @12:18:33.57 AS.seek :empty @12:19:21.06 PCR.program :bleachProfile @12:19:21.14 PCR program should complete in < 2:45 @12:19:21.16 PCR.run 10:00 @12:22:47.25 PCR.off @12:22:47.29 AFV.select :carrierSolution @12:22:47.97 delay 2 seconds @12:22:50.00 AS.seek 1ml @12:23:47.87 delay 1 second @12:23:48.88 AFV.select :holdingCoil @12:23:49.02 delay 1 second @12:23:50.05 AS.reconfigure ASfast @12:23:50.12 AS.seek 0ml @12:24:17.48 delay 3 seconds @12:24:20.50 AFV.select :carrierSolution @12:24:20.64 delay 2 seconds @12:24:22.67 AS.reconfigure ASconfig @12:24:22.75 AS.seek 1ml @12:25:20.56 delay 1 second @12:25:21.57 AFV.select :holdingCoil @12:25:21.71 delay 1 second @12:25:22.75 AS.reconfigure ASfast @12:25:22.82 AS.seek 0ml @12:25:50.19 delay 3 seconds @12:25:53.21 AFV.select :carrierSolution @12:25:53.35 delay 2 seconds @12:25:55.38 AS.reconfigure ASconfig @12:25:55.46 AS.seek 1ml @12:26:53.28 delay 1 second @12:26:54.29 AFV.select :holdingCoil @12:26:54.42 delay 1 second @12:26:55.45 AS.reconfigure ASfast @12:26:55.52 AS.seek 0ml @12:27:22.89 delay 3 seconds @12:27:25.91 AFV.select :carrierSolution @12:27:26.05 delay 2 seconds @12:27:28.08 AS.reconfigure ASconfig @12:27:28.15 AS.seek 1ml @12:28:25.97 delay 1 second @12:28:26.98 AFV.select :holdingCoil @12:28:27.11 delay 1 second @12:28:28.14 AS.reconfigure ASfast @12:28:28.22 AS.seek 0ml @12:28:55.58 delay 3 seconds @12:28:58.60 AFV.select :carrierSolution @12:28:58.74 delay 2 seconds @12:29:00.77 AS.reconfigure ASconfig @12:29:00.86 AS.seek 1ml @12:29:58.67 delay 1 second @12:29:59.68 AFV.select :holdingCoil @12:29:59.81 delay 1 second @12:30:00.84 AS.reconfigure ASnorm @12:30:00.91 AS.seek 0ml @12:42:52.07 -> ESP.shutdown @12:42:52.11 AS.coast @12:42:52.15 Asleep / Shutdown @12:42:52.23 SS.coast @12:42:52.29 CC.coast @12:42:52.34 CS.coast @12:42:52.40 PC.coast @12:42:52.46 Forearm.coast @12:42:52.53 Elbow.coast @12:42:52.59 SC.coast @12:42:52.65 SE.coast @12:42:53.06 Gate.power :core,:OFF @12:42:53.08 Gate.power :raw,:OFF @12:42:53.10 Shutdown @12:43:03.25 -> exit @12:43:03.26 Session Ended @14:00:51.53
Powering core bus @14:00:56.48 Gate.power :raw,:ON @14:00:57.68 SPE.coast @14:00:57.74 SPE.configure SPEconfig @14:00:57.83 AS.configure ASconfig @14:00:57.93 SS.configure SSconfig @14:00:58.03 CC.configure CCconfig @14:00:58.13 CS.configure CSconfig @14:00:58.23 PC.configure PCconfig @14:00:58.33 PS.configure PSconfig @14:00:58.45 Forearm.configure LooseForearm @14:00:58.55 Elbow.configure LooseElbow @14:00:58.67 SC.configure SCloose @14:00:58.76 SE.configure SEconfig @14:00:58.81 CH.coast @14:00:58.86 CH.configure CHconfig @14:00:58.90 PH.coast @14:00:58.96 PH.configure PHconfig @14:00:59.00 PCR.off @14:00:59.03 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:59.05 PCR.caltemp= 2.7073,0.0373,0 @14:00:59.08 All dwarf controllers running firmware version 3.75 @14:00:59.20 Valve.reset @14:00:59.37 PCR.off @14:00:59.40 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:59.42 PCR.caltemp= 2.7073,0.0373,0 @14:00:59.65 PSV.dial :air @14:00:59.68 Intake.select :close @14:00:59.71 Exhaust.select :close @14:00:59.74 CTV.dial :air @14:00:59.86 PS.reconfigure PSconfig @14:00:59.99 PS.home.seek 0ml @14:01:00.03 CSV.dial :waste1 @14:01:00.06
Forearm.reconfigure LooseForearm @14:01:00.19 Forearm.home.seek :retract @14:01:00.26 SSV.dial :spit,avoiding: :exhaust @14:01:00.41 CS.reconfigure CSfast @14:01:00.44 SS.reconfigure SSconfig @14:01:00.54 CS.home.seek 0ml @14:01:00.57 SS.home.seek 0ml @14:01:01.48 Hand.close @14:01:01.53
Elbow.jog 1500 @14:01:02.07 Elbow.reconfigure LooseElbow @14:01:02.12 Elbow.home.seek :clear @14:01:06.79 SE.reconfigure SEconfig @14:01:06.83 SE.home.seek :down @14:01:07.58 SC.reconfigure SCsloppy @14:01:07.63 SC.home.seek :initial @14:01:09.08 PS.coast @14:01:11.60
Forearm.seek :retract @14:01:11.68 Elbow.seek :hammer @14:01:12.96 Forearm.reconfigure TightForearm @14:01:13.01 Forearm.seek 5 + 350 counts @14:01:14.14 Forearm.seek :retract @14:01:15.34 PC.reconfigure PCconfig @14:01:15.35
CC.reconfigure CCconfig @14:01:15.44 CC.home.seek :open @14:01:15.48 PC.home.seek :open @14:01:19.00 GoodNews.email "At 14:01:18 began in tube 3: mbaPhase\n/home/esp/esp2/mission/18tundeployv3.rb:123\n{1 => 22, 2 => 14, 3 => 8}\nCan@14:01:18, 13.5C, 71% humidity, 14.3psia, 13.151V, 0.771A, 0.771A avg, 10.14W",Subject: "Started 18tundeployv3 phase mbaPhase" @14:01:19.04
Phase [mbaPhase] @14:01:19.06CEST15-Oct-18 ==================> STARTED Superphase (phase #3) <=== @14:01:19.07 Starting initialPurge @14:01:19.09 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:01:19.16 Hand.open @14:01:19.93 Elbow.reconfigure TightElbow @14:01:19.98 Elbow.seek FlushPuck::Garage @14:01:21.92 Elbow.reconfigure LooseElbow @14:01:22.02 Forearm.reconfigure LooseForearm @14:01:22.07 Forearm.seek :garage @14:01:23.04 Hand.close @14:01:23.85 Forearm.seek :retract @14:01:24.86 Elbow.reconfigure TightElbow @14:01:24.91 Elbow.seek CC @14:01:27.82 Elbow.reconfigure LooseElbow @14:01:27.93 Forearm.reconfigure TightForearm @14:01:27.98 Forearm.seek CC @14:01:29.29 CC.seek :closed @14:01:33.57 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.99 @14:01:33.65 Hand.open @14:01:34.38 Forearm.reconfigure LooseForearm @14:01:34.42 Forearm.seek :retract @14:01:35.40 Flushing the Collection Valve::Manifold @14:01:35.53 CSV.dial CSR @14:01:36.96 Pulling 5ml of CSR.flush @14:01:36.97 CSR.select :flush @14:01:37.10 CS.reconfigure CSconfig @14:01:37.15 CS.seek 5ml @14:01:50.98 delay 5 seconds @14:01:56.00 CSR.select :air @14:01:56.17 CS.reconfigure CSfast @14:01:56.22 CS.seek 7.5ml @14:02:03.06 CSV.dial :waste @14:02:04.43 CS.reconfigure CSconfig @14:02:04.48 CS.seek 0ml @14:02:24.57 Purging external Intake @14:02:24.70 CSV.dial :flush @14:02:25.18 CS.reconfigure CSfast @14:02:25.23 CS.seek 10ml @14:02:49.76 delay 5 seconds @14:02:54.78 CSV.dial CBV @14:02:54.82
CBV.dial CSV @14:02:55.62 CTV.dial :intake @14:02:56.42 Intake.select :open @14:02:56.55 CS.seek 0ml @14:03:22.09 Intake.select :close @14:03:22.21 CTV.dial :air @14:03:23.01 Flushing upper manifold @14:03:23.14 CSV.dial :flush @14:03:23.93 CS.seek 5ml @14:03:37.26 delay 5 seconds @14:03:42.28 CSV.dial :air @14:03:42.83 CS.seek 7ml @14:03:48.57 CSV.dial CBV @14:03:48.61
CBV.dial CSV @14:03:49.70 CTV.dial :air @14:03:49.72 CS.seek 0ml @14:04:07.01 delay 7 seconds @14:04:14.04 CS.seek 3ml @14:04:22.03 CSV.dial :waste @14:04:22.51 CS.seek 0ml @14:04:30.63 CSV.dial :flush @14:04:31.10 CS.seek 3ml @14:04:39.10 delay 5 seconds @14:04:44.12 CSV.dial :air @14:04:44.67 CS.seek 5ml @14:04:50.44 CSV.dial :air @14:04:50.47 CS.seek 0ml @14:05:03.04 delay 7 seconds @14:05:10.08 CS.seek 3ml @14:05:18.19 CSV.dial :waste @14:05:18.98 CS.seek 0ml @14:05:27.01 Garaging FlushPuck in Collection Clamp @14:05:27.09 Elbow.reconfigure TightElbow @14:05:27.14 Elbow.seek CC @14:05:27.40 Elbow.reconfigure LooseElbow @14:05:27.50 Forearm.seek CC @14:05:28.47 Hand.close @14:05:29.06 CC.seek :open @14:05:33.42 Forearm.seek :retract @14:05:34.39 Elbow.reconfigure TightElbow @14:05:34.44 Elbow.seek :garage @14:05:37.51 Elbow.reconfigure LooseElbow @14:05:37.62 Forearm.reconfigure TightForearm @14:05:37.66 Forearm.seek :garage @14:05:38.78 Hand.open @14:05:39.49 Forearm.reconfigure LooseForearm @14:05:39.54 Forearm.seek :retract @14:05:40.68 Starting mfb @14:05:40.70 pcrBAC sampling at most 150ml, wcr at most 1500ml @14:05:40.74 BACfans.email "Started mfb in tube 3 at 14:05:40CEST15-Oct-18\n{1 => 22, 2 => 14, 3 => 8}\nCan@14:01:18, 13.5C, 71% humidity, 14.3psia, 13.151V, 0.771A, 0.771A avg, 10.14W",Subject: "pcrBAC sampling at most 150ml, wcr at most 1500ml" @14:05:40.81
Loading sh1 puck from tube 3 into Collection Clamp @14:05:40.93 Readying the MFB @14:05:40.98 Homing MFB Syringe @14:05:41.00 AFV.select :holdingCoil @14:05:41.24 ATV.dial :air2 @14:05:41.27
SC.reconfigure SCtight @14:05:41.37 SC.seek 3 @14:05:42.68 AS.reconfigure ASconfig @14:05:42.74 AS.home.seek :home @14:05:43.43
SC.reconfigure SCloose @14:05:43.71 Elbow.reconfigure TightElbow @14:05:43.76 Elbow.seek 3 @14:05:45.82 Forearm.reconfigure TightForearm @14:05:45.88 Forearm.seek 3 + 700 counts @14:05:46.93 SE.reconfigure SEpick @14:05:47.01 SE.seek beamBreak @14:05:48.26 AFV.select :carrierSolution @14:05:48.38 delay 2 seconds @14:05:49.47
SE.configure SEconfig @14:05:49.65 Elbow.seek 3 @14:05:50.04 Forearm.seek 3 + 300 counts @14:05:50.31 Forearm.reconfigure LooseForearm @14:05:50.37 Hand.close @14:05:50.43 AS.seek 1ml @14:05:50.95 SE.seek :down @14:05:51.19
Forearm.seek :retract @14:05:52.14 Elbow.seek CC @14:05:53.06 SE.coast @14:05:53.71
Elbow.reconfigure LooseElbow @14:05:53.83 Forearm.reconfigure TightForearm @14:05:53.88 Forearm.seek CC @14:05:55.17 CC.seek :closed @14:05:59.45 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.07 @14:05:59.46 Hand.open @14:06:00.13 Forearm.reconfigure LooseForearm @14:06:00.17 Forearm.seek :retract @14:06:01.15 SC.coast @14:06:01.19 collectSample 150ml w/bubble point of 55psi @14:06:01.20 Priming 7ml @14:06:01.21 Intake.select :close @14:06:01.22 Exhaust.select :close @14:06:01.23 SS.hold @14:06:01.46 Calibrating pressure and temperature sensors @14:06:01.48 CTV.dial :air @14:06:01.49 SSV.dial :spit,avoiding: :exhaust @14:06:01.52 SS.reconfigure SSfast @14:06:01.56 SS.seek 5ml @14:06:04.29 SSV.dial CBV @14:06:04.32
CBV.dial SSV @14:06:05.73 delay 3.8 seconds @14:06:09.67 SSV.dial :spit @14:06:11.07 SS.seek 0ml @14:06:13.73 SSV.dial CBV @14:06:15.14 CTV.dial :intake @14:06:15.95 SSV.dial CBV @14:06:15.98
CBV.dial SSV @14:06:15.99 Allowing 40s for 7.0ml of remaining 7.0ml @14:06:16.00 Intake.select :open @14:06:18.18 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:06:18.20 SS.reconfigure SSinhale.with maxSpeed: 150 @14:06:18.25 SS.seek 7ml @14:06:21.68 Vacuum too high -- waiting up to 40s @14:06:23.04 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:06:23.06 SS.reconfigure SSinhale.with maxSpeed: 135 @14:06:23.11 SS.seek 7ml @14:06:25.87 Vacuum too high -- waiting up to 40s @14:06:27.48 SS.reconfigure SSinhale.with maxSpeed: 122 @14:06:27.53 SS.seek 7ml @14:06:29.42 Stroke completed -- waiting for pressure to equalize ... @14:06:31.39 Intake.select :close @14:06:31.55 SSV.dial :exhaust @14:06:32.34 Exhaust.select :open @14:06:32.46 SS.reconfigure SSexhale @14:06:32.51 SS.seek 0ml @14:06:36.08 Exhaust.select :close @14:06:36.20 Sampling 150.0ml @14:06:36.21 Intake.select :close @14:06:36.22 Exhaust.select :close @14:06:36.24 SS.reconfigure SSconfig @14:06:36.28 SS.hold @14:06:36.50 CTV.dial :intake @14:06:36.52 SSV.dial CBV @14:06:36.54
CBV.dial SSV @14:06:37.39 Allowing 130s for 25.0ml of remaining 150.0ml @14:06:37.40 Intake.select :open @14:06:39.57 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:06:39.59 SS.reconfigure SSinhale.with maxSpeed: 122 @14:06:39.64 SS.seek 25ml @14:06:43.35 delay 1 second @14:06:43.52
Vacuum too high -- waiting up to 40s @14:06:44.36 AFV.select :holdingCoil @14:06:44.51 delay 1 second @14:06:44.87
Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:06:44.89 SS.reconfigure SSinhale.with maxSpeed: 110 @14:06:44.94 SS.seek 25ml @14:06:45.54 ATV.dial :waste @14:06:46.54 AS.reconfigure ASnorm @14:06:46.59 AS.home.seek :empty @14:06:47.65
Vacuum too high -- waiting up to 40s @14:06:49.01 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:06:49.03 SS.reconfigure SSinhale.with maxSpeed: 99 @14:06:49.08 SS.seek 25ml @14:06:51.77 Vacuum too high -- waiting up to 40s @14:06:53.10 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:06:53.12 SS.reconfigure SSinhale.with maxSpeed: 89 @14:06:53.17 SS.seek 25ml @14:06:55.96 Vacuum too high -- waiting up to 40s @14:06:57.85 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:06:57.87 SS.reconfigure SSinhale.with maxSpeed: 80 @14:06:57.91 SS.seek 25ml @14:07:01.39 Vacuum too high -- waiting up to 40s @14:07:02.49 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:07:02.51 SS.reconfigure SSinhale.with maxSpeed: 72 @14:07:02.56 SS.seek 25ml @14:07:05.88 Vacuum too high -- waiting up to 40s @14:07:07.03 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:07:07.05 SS.reconfigure SSinhale.with maxSpeed: 65 @14:07:07.10 SS.seek 25ml @14:07:11.50 Vacuum too high -- waiting up to 40s @14:07:12.59 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:07:12.61 SS.reconfigure SSinhale.with maxSpeed: 59 @14:07:12.66 SS.seek 25ml @14:07:17.65 Vacuum too high -- waiting up to 40s @14:07:18.75 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:07:18.77 SS.reconfigure SSinhale.with maxSpeed: 53 @14:07:18.82 SS.seek 25ml @14:07:20.56 Stroke completed -- waiting for pressure to equalize ... @14:07:22.53 Intake.select :close @14:07:22.70 SSV.dial :exhaust @14:07:23.52 Exhaust.select :open @14:07:23.64 SS.reconfigure SSexhale @14:07:23.69 SS.seek 0ml @14:07:32.52 Exhaust.select :close @14:07:32.64 SSV.dial CBV @14:07:32.67
CBV.dial SSV @14:07:33.51 Allowing 130s for 25.0ml of remaining 125.0ml @14:07:33.52 Intake.select :open @14:07:35.69 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:07:35.71 SS.reconfigure SSinhale.with maxSpeed: 53 @14:07:35.76 SS.seek 25ml @14:07:39.14 ATV.dial :waste @14:07:39.16 ARV.dial between carrier and air1 @14:07:39.24 ASV.dial :waste1 @14:07:46.18
Vacuum too high -- waiting up to 40s @14:07:47.00 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:07:47.01 SS.reconfigure SSinhale.with maxSpeed: 48 @14:07:47.06 SS.seek 25ml @14:08:13.84 Stroke completed -- waiting for pressure to equalize ... @14:08:17.02 Intake.select :close @14:08:17.17 SSV.dial :exhaust @14:08:17.98 Exhaust.select :open @14:08:18.10 SS.reconfigure SSexhale @14:08:18.15 SS.seek 0ml @14:08:27.20 Exhaust.select :close @14:08:27.32 SSV.dial CBV @14:08:27.88
CBV.dial SSV @14:08:28.73 Allowing 130s for 25.0ml of remaining 100.0ml @14:08:28.74 Intake.select :open @14:08:30.91 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:08:30.93 SS.reconfigure SSinhale.with maxSpeed: 48 @14:08:30.98 SS.seek 25ml @14:08:54.68 Vacuum too high -- waiting up to 40s @14:08:55.53 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:08:55.54 SS.reconfigure SSinhale.with maxSpeed: 43 @14:08:55.59 SS.seek 25ml @14:09:10.75 Stroke completed -- waiting for pressure to equalize ... @14:09:13.34 Intake.select :close @14:09:13.50 SSV.dial :exhaust @14:09:14.30 Exhaust.select :open @14:09:14.42 SS.reconfigure SSexhale @14:09:14.47 SS.seek 0ml @14:09:23.23 Exhaust.select :close @14:09:23.35 SSV.dial CBV @14:09:23.38
CBV.dial SSV @14:09:24.21 Allowing 130s for 25.0ml of remaining 75.0ml @14:09:24.22 Intake.select :open @14:09:26.39 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:09:26.41 SS.reconfigure SSinhale.with maxSpeed: 43 @14:09:26.46 SS.seek 25ml @14:10:08.10 Stroke completed -- waiting for pressure to equalize ... @14:10:10.67 Intake.select :close @14:10:10.83 SSV.dial :exhaust @14:10:11.64 Exhaust.select :open @14:10:11.76 SS.reconfigure SSexhale @14:10:11.81 SS.seek 0ml @14:10:20.91 Exhaust.select :close @14:10:21.03 SSV.dial CBV @14:10:21.06
CBV.dial SSV @14:10:21.90 Allowing 130s for 25.0ml of remaining 50.0ml @14:10:21.91 Intake.select :open @14:10:24.08 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:10:24.10 SS.reconfigure SSinhale.with maxSpeed: 43 @14:10:24.15 SS.seek 25ml @14:11:05.89 Stroke completed -- waiting for pressure to equalize ... @14:11:08.46 Intake.select :close @14:11:08.61 SSV.dial :exhaust @14:11:09.41 Exhaust.select :open @14:11:09.53 SS.reconfigure SSexhale @14:11:09.59 SS.seek 0ml @14:11:18.81 Exhaust.select :close @14:11:18.93 SSV.dial CBV @14:11:18.96
CBV.dial SSV @14:11:19.79 Allowing 130s for 25.0ml of remaining 25.0ml @14:11:19.80 Intake.select :open @14:11:21.97 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:11:21.99 SS.reconfigure SSinhale.with maxSpeed: 43 @14:11:22.04 SS.seek 25ml @14:12:03.30 Stroke completed -- waiting for pressure to equalize ... @14:12:05.87 Intake.select :close @14:12:06.02 SSV.dial :exhaust @14:12:06.83 Exhaust.select :open @14:12:06.95 SS.reconfigure SSexhale @14:12:07.00 SS.seek 0ml @14:12:16.13 Exhaust.select :close @14:12:16.25 Sampled 150.0ml @14:12:16.27 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @14:12:16.86 CSV.dial :waste @14:12:16.93 CS.seek 0ml @14:12:17.04 SSV.dial CBV,avoiding: :exhaust @14:12:17.06
CBV.dial SSV @14:12:17.14 CSV.dial :air @14:12:17.95 CS.seek 10ml @14:12:19.06
CTV.dial :air @14:12:19.88 delay 15 seconds @14:12:34.91 SSV.dial :spit,avoiding: :exhaust @14:12:36.32 CTV.dial CSV,avoiding: :intake @14:12:36.41
SS.seek 0ml @14:12:36.49 SSV.dial CBV,avoiding: :exhaust @14:12:42.73 CBV.dial between SSV and waste2 @14:12:43.15 CTV.dial CSV,avoiding: :air @14:12:43.18
CSV.dial CTV @14:12:43.66 CS.reconfigure CSpressurize @14:12:43.71 CS.seek 0ml @14:13:09.85 CSV.dial :air @14:13:10.35 CS.reconfigure CSfast @14:13:10.40 CS.seek 10ml @14:13:34.70 CSV.dial between air and CTV @14:13:35.06 CS.seek 2.048ml @14:13:54.61 CTV.dial CSV,avoiding: :air @14:13:54.64
CSV.dial CTV @14:13:54.97 CS.reconfigure CSpressurize @14:13:55.02 CS.seek 0ml @14:13:56.29 BubblePoint: Intake @69.6psia, Outlet @14.6psia @14:13:56.30 CBV.dial SSV @14:13:56.66 SS.reconfigure SSconfig @14:13:56.71 SS.seek 10ml @14:14:02.40 delay 15 seconds @14:14:17.55 Evacuated: Intake @44.7psia, Outlet @1.2psia @14:14:17.56 SSV.dial :spit,avoiding: :exhaust @14:14:18.96 SS.reconfigure SSexhale @14:14:19.01 SS.seek 0ml @14:14:24.22 SS.reconfigure SSfast @14:14:24.27 SS.seek 5ml @14:14:27.06 SSV.dial CBV,avoiding: :exhaust @14:14:27.09
CBV.dial SSV @14:14:28.51 CTV.dial :air,avoiding: :intake @14:14:29.91 CSV.dial CTV @14:14:29.96
SS.seek 10ml @14:14:30.95 CSV.dial :air @14:14:31.46 CS.reconfigure CSfast @14:14:31.51 CS.seek 0ml @14:14:32.53
SSV.dial :spit,avoiding: :exhaust @14:14:33.95 SS.reconfigure SSexhale @14:14:34.00 SS.seek 0ml @14:14:36.67 CS.coast @14:14:39.24
SS.reconfigure SSfast @14:14:39.29 SS.seek 5ml @14:14:41.88 SSV.dial CBV,avoiding: :exhaust @14:14:41.91
CBV.dial SSV @14:14:44.32 SSV.dial :spit,avoiding: :exhaust @14:14:45.71 SS.reconfigure SSconfig @14:14:45.76 SS.seek 0ml @14:14:48.92 SS.coast @14:14:48.97 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @14:14:48.98 Intake.select :close @14:14:49.57 CSV.dial :waste @14:14:49.71 SSV.dial CBV,avoiding: :exhaust @14:14:49.73
CBV.dial SSV @14:14:50.42 CS.seek 0ml @14:14:50.49 CSV.dial :air @14:14:51.15
CTV.dial :air @14:14:51.16 delay 15 seconds @14:14:51.28 CS.seek 10ml @14:15:06.19
SSV.dial :spit,avoiding: :exhaust @14:15:07.60 CTV.dial CSV,avoiding: :intake @14:15:07.66
SS.reconfigure SSexhale @14:15:07.75 SS.seek 0ml @14:15:08.04 SSV.dial CBV,avoiding: :exhaust @14:15:15.77 CBV.dial between SSV and waste2 @14:15:16.20 CSV.dial between air and CTV @14:15:16.54 CS.seek 8.045ml @14:15:22.14 CTV.dial CSV,avoiding: :air @14:15:22.17
CSV.dial CTV @14:15:22.50 CS.reconfigure CSpressurize @14:15:22.55 CS.seek 0ml @14:15:43.79 CSV.dial :air @14:15:44.27 CS.reconfigure CSfast @14:15:44.32 CS.seek 10ml @14:16:08.64 CSV.dial between air and CTV @14:16:09.00 CS.seek 3.102ml @14:16:25.99 CTV.dial CSV,avoiding: :air @14:16:26.02
CSV.dial CTV @14:16:26.35 CS.reconfigure CSpressurize @14:16:26.40 CS.seek 0ml @14:16:34.47 BubblePoint: Intake @69.2psia, Outlet @13.9psia @14:16:34.48 CBV.dial SSV @14:16:34.83 SS.reconfigure SSconfig @14:16:34.88 SS.seek 10ml @14:16:40.29 delay 15 seconds @14:16:55.43 Evacuated: Intake @40.4psia, Outlet @15.1psia @14:16:55.44 SSV.dial :spit,avoiding: :exhaust @14:16:56.84 SS.reconfigure SSexhale @14:16:56.89 SS.seek 0ml @14:17:01.86 SS.reconfigure SSfast @14:17:01.91 SS.seek 5ml @14:17:04.60 SSV.dial CBV,avoiding: :exhaust @14:17:04.63
CBV.dial SSV @14:17:06.06 CTV.dial :air,avoiding: :intake @14:17:07.46 CSV.dial CTV @14:17:07.51
SS.seek 10ml @14:17:08.50 CSV.dial :air @14:17:09.01 CS.reconfigure CSfast @14:17:09.06 CS.seek 0ml @14:17:10.01
SSV.dial :spit,avoiding: :exhaust @14:17:10.79 CS.coast @14:17:11.42
SS.reconfigure SSexhale @14:17:11.47 SS.seek 0ml @14:17:16.47 SS.reconfigure SSfast @14:17:16.52 SS.seek 5ml @14:17:19.18 SSV.dial CBV,avoiding: :exhaust @14:17:19.21
CBV.dial SSV @14:17:21.61 SSV.dial :spit,avoiding: :exhaust @14:17:23.53 SS.reconfigure SSconfig @14:17:23.58 SS.seek 0ml @14:17:26.74 SS.coast @14:17:26.79 Lysing: Adding 1.5ml lysis @14:17:26.80 CBV.dial between CSV and waste1,avoiding: :DBV @14:17:28.40 CTV.dial CSV @14:17:29.79 CSV.dial CSR @14:17:30.63 Pulling 1ml of CSR.lysis @14:17:30.64 CSR.select :lysis @14:17:30.76 CS.reconfigure CSconfig @14:17:30.80 CS.seek 1ml @14:17:34.30 delay 5 seconds @14:17:39.32 CSR.select :air @14:17:39.50 CS.seek 6ml @14:17:53.33 delay 2 seconds @14:17:55.34 CSV.dial :waste1 @14:17:56.71 CS.reconfigure CSfast @14:17:56.76 CS.seek :empty @14:18:11.70 CSV.dial CSR @14:18:13.15 Pulling 1.5ml of CSR.lysis @14:18:13.16 CSR.select :lysis @14:18:13.28 CS.reconfigure CSconfig @14:18:13.32 CS.seek 1.5ml @14:18:18.15 delay 5 seconds @14:18:23.17 CSR.select :air @14:18:23.35 CS.seek 4.5ml @14:18:31.96 delay 2 seconds @14:18:33.97 CSV.dial CTV @14:18:34.46 CS.seek 1.275ml @14:18:43.74 Heating sh1 puck from tube 3 in Collection Clamp to 85C for 5:00 @14:18:43.76 CH.seek 85C,8:00 @14:21:48.24 CH.hold 5:00,85C @14:26:48.51 Collection Puck Heater at 85.1C after lysing @14:26:48.53 CS.seek 0ml @14:26:52.97 CTV.dial between spare and air,avoiding: :intake @14:26:54.09 CSV.dial :air @14:26:54.59 CS.seek 5ml @14:27:08.27 CSV.dial CTV @14:27:08.89 delay 2 seconds @14:27:10.92 CSV.dial :air @14:27:11.37 delay 2 seconds @14:27:13.39 CSV.dial CTV @14:27:13.87 delay 2 seconds @14:27:15.89 CSV.dial :air @14:27:16.37 delay 2 seconds @14:27:18.39 CS.seek 0ml @14:27:32.09 Recovering Lysate into Collection syringe @14:27:32.10 CSV.dial CBV @14:27:33.30 CBV.dial CSV @14:27:33.71 CS.seek 5ml @14:27:47.66 delay 5 seconds @14:27:52.68 CTV.dial :air @14:27:53.24 CS.seek 7ml @14:27:59.31 delay 5 seconds @14:28:04.34 CSV.dial :air2 @14:28:04.83 CS.seek 10ml @14:28:13.47 CSV.dial between air and CTV,avoiding: CBV @14:28:14.81 Lysing Complete @14:28:14.89 Storing sh1 puck in Collection Clamp to tube 2 @14:28:15.08 SC.reconfigure SCtight @14:28:15.13 SC.seek 2 @14:28:17.41 SC.reconfigure SCloose @14:28:17.63 Elbow.reconfigure TightElbow @14:28:17.68 Elbow.seek 2 @14:28:19.52 Forearm.reconfigure TightForearm @14:28:19.57 Forearm.seek 2 + 700 counts @14:28:20.61 SE.reconfigure SEpick @14:28:20.68 SE.seek beamBreak @14:28:22.50 SE.configure SEconfig @14:28:22.67 Elbow.reconfigure LimpElbow @14:28:22.73 Forearm.reconfigure LooseForearm @14:28:22.77 Forearm.seek 2 @14:28:23.04 Elbow.hold @14:28:23.11 Elbow.reconfigure TightElbow @14:28:23.22 SE.seek 13.09 Pucks below Top Plate @14:28:23.75 Forearm.force -20 @14:28:23.79 Forearm.seek 2 + 350 counts @14:28:24.10 Forearm.reconfigure TightForearm @14:28:24.15 Forearm.seek 2 + 700 counts @14:28:24.41 SE.seek 14.09 Pucks below Top Plate @14:28:25.08 Forearm.reconfigure LooseForearm @14:28:25.13 Forearm.seek :retract @14:28:26.08 Elbow.seek CC @14:28:28.31 Elbow.reconfigure LooseElbow @14:28:28.42 Forearm.seek CC @14:28:29.36 Hand.close @14:28:29.97 CC.seek :open @14:28:34.48 Forearm.seek :retract @14:28:35.48 Elbow.reconfigure TightElbow @14:28:35.53 Elbow.seek 2 @14:28:37.39 Elbow.reconfigure LimpElbow @14:28:37.44 Forearm.seek 2 @14:28:38.48 Elbow.hold @14:28:38.55 Elbow.reconfigure TightElbow @14:28:38.60 Hand.open @14:28:39.35 Forearm.force -20 @14:28:39.40 Forearm.seek 2 + 347 counts @14:28:39.72 Forearm.reconfigure TightForearm @14:28:39.78 Forearm.seek 2 + 700 counts @14:28:40.11 SE.seek :down @14:28:41.28
Forearm.reconfigure LooseForearm @14:28:41.33 Forearm.seek :retract @14:28:41.51 SE.coast @14:28:42.23
SC.coast @14:28:42.27 Tranferring lysate to PRV @14:28:42.28 CSV.dial :air @14:28:42.76 CS.seek 10ml @14:28:42.84 CSV.dial between air and CTV @14:28:43.17 AIV.dial :bypass @14:28:43.25 Beginning samplePassC @14:28:43.26 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:28:43.34 Hand.open @14:28:43.73 Elbow.seek FlushPuck::Garage @14:28:45.71 Elbow.reconfigure LooseElbow @14:28:45.82 Forearm.seek :garage @14:28:46.80 Hand.close @14:28:47.60 Forearm.seek :retract @14:28:48.59 Elbow.reconfigure TightElbow @14:28:48.64 Elbow.seek CC @14:28:51.46 Elbow.reconfigure LooseElbow @14:28:51.57 Forearm.reconfigure TightForearm @14:28:51.62 Forearm.seek CC @14:28:52.86 CC.seek :closed @14:28:57.11 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.08 @14:28:57.20 Hand.open @14:28:57.91 Forearm.reconfigure LooseForearm @14:28:57.96 Forearm.seek :retract @14:28:58.93 AWV.select :air @14:28:58.95 AIV.dial :bypass @14:28:58.96 PSV.dial PSR @14:29:00.39 Pulling 1.5ml of PSR.flush @14:29:00.40 PSR.select :flush @14:29:00.53 PS.seek 1.5ml @14:29:16.51 delay 5 seconds @14:29:21.53 PSV.dial :waste1 @14:29:22.03 PS.seek 0ml @14:29:38.13 PSV.dial PSR @14:29:38.60 PSR.select :air @14:29:38.74 PS.seek 2.5ml @14:30:04.83 PRV.dial CBV @14:30:04.86
CBV.dial PRV @14:30:05.73 PSV.dial :mixloop @14:30:06.81 CTV.dial :air,avoiding: :intake @14:30:06.82 PTV.dial :PRVmixing @14:30:06.91 PS.seek 0ml @14:30:33.04 PSV.dial :air @14:30:33.53 PS.seek 2.5ml @14:30:59.60 PSV.dial :mixloop @14:31:00.08 PS.seek 0ml @14:31:26.26 PSV.dial :air @14:31:26.75 PS.seek 2.5ml @14:31:52.91 PSV.dial :mixloop @14:31:53.39 PS.seek 0ml @14:32:19.58 Garaging FlushPuck in Collection Clamp @14:32:19.66 Elbow.reconfigure TightElbow @14:32:19.71 Elbow.seek CC @14:32:19.94 Elbow.reconfigure LooseElbow @14:32:20.05 Forearm.seek CC @14:32:21.07 Hand.close @14:32:21.72 CC.seek :open @14:32:26.07 Forearm.seek :retract @14:32:27.07 Elbow.reconfigure TightElbow @14:32:27.12 Elbow.seek :garage @14:32:30.24 Elbow.reconfigure LooseElbow @14:32:30.35 Forearm.reconfigure TightForearm @14:32:30.40 Forearm.seek :garage @14:32:31.57 Hand.open @14:32:32.29 Forearm.reconfigure LooseForearm @14:32:32.33 Forearm.seek :retract @14:32:33.36 Connecting PS to CS to transfer sample @14:32:33.37 CBV.dial AIV @14:32:33.38 AIV.dial :bypass @14:32:33.39 PSV.dial :mixloop @14:32:33.40 PTV.dial :PRVmixing @14:32:33.42 PRV.dial AIV @14:32:33.43 CTV.dial :air,avoiding: :intake @14:32:33.45 Loading lyfil puck from tube 3 into Collection Clamp @14:32:33.71 SC.reconfigure SCtight @14:32:33.76 SC.seek 3 @14:32:35.91 SC.reconfigure SCloose @14:32:36.14 Elbow.reconfigure TightElbow @14:32:36.19 Elbow.seek 3 @14:32:38.76 Forearm.reconfigure TightForearm @14:32:38.82 Forearm.seek 3 + 700 counts @14:32:39.89 SE.reconfigure SEpick @14:32:39.96 SE.seek beamBreak @14:32:42.48 SE.configure SEconfig @14:32:42.65 Elbow.seek 3 @14:32:42.85 Forearm.seek 3 + 300 counts @14:32:43.12 Forearm.reconfigure LooseForearm @14:32:43.16 Hand.close @14:32:43.87 SE.seek :down @14:32:44.11
Forearm.seek :retract @14:32:45.02 Elbow.seek CC @14:32:46.26 SE.coast @14:32:46.69
Elbow.reconfigure LooseElbow @14:32:46.80 Forearm.reconfigure TightForearm @14:32:46.85 Forearm.seek CC @14:32:48.13 CC.seek :closed @14:32:52.38 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.11 @14:32:52.39 Hand.open @14:32:53.04 Forearm.reconfigure LooseForearm @14:32:53.08 Forearm.seek :retract @14:32:54.06 SC.coast @14:32:54.10 CTV.dial CSV,avoiding: :intake @14:32:55.50 CSV.dial CTV @14:32:55.83 CS.seek 5ml @14:33:09.63 delay 1:20 @14:34:29.66 CS.seek 2.5ml @14:34:37.01 delay 40 seconds @14:35:17.04 CS.seek 0ml @14:35:24.76 delay 1:00 @14:36:24.79 PSV.dial :air @14:36:25.28 PS.seek 2.5ml @14:36:51.42 PSV.dial :mixloop @14:36:51.91 delay 3 seconds @14:36:54.92 PSV.dial :air @14:36:55.41 delay 2 seconds @14:36:57.42 PSV.dial :mixloop @14:36:57.91 delay 3 seconds @14:37:00.92 PSV.dial :waste1 @14:37:02.32 PS.seek :empty @14:37:28.46 CBV.dial :waste1 @14:37:28.96 delay 5 seconds @14:37:33.98 CBV.dial AIV @14:37:34.49 delay 10 seconds @14:37:44.51 CBV.dial :waste1 @14:37:45.02 delay 10 seconds @14:37:55.04 CBV.dial AIV @14:37:55.56 delay 10 seconds @14:38:05.59 CTV.dial CSV,avoiding: :intake @14:38:05.60 CBV.dial between AIV and waste1 @14:38:05.98 CSV.dial :air @14:38:06.48 CS.seek 10ml @14:38:33.00 CSV.dial CTV @14:38:33.49 CS.seek 0ml @14:39:00.13 CBV.dial :waste1 @14:39:00.48 delay 10 seconds @14:39:10.51 CTV.dial :air,avoiding: :intake @14:39:12.50 Storing lyfil puck in Collection Clamp to tube 2 @14:39:12.69 SC.reconfigure SCtight @14:39:12.74 SC.seek 2 @14:39:14.88 SC.reconfigure SCloose @14:39:15.11 Elbow.reconfigure TightElbow @14:39:15.16 Elbow.seek 2 @14:39:17.16 Forearm.reconfigure TightForearm @14:39:17.21 Forearm.seek 2 + 700 counts @14:39:18.30 SE.reconfigure SEpick @14:39:18.37 SE.seek beamBreak @14:39:20.15 SE.configure SEconfig @14:39:20.32 Elbow.reconfigure LimpElbow @14:39:20.37 Forearm.reconfigure LooseForearm @14:39:20.41 Forearm.seek 2 @14:39:20.73 Elbow.hold @14:39:20.80 Elbow.reconfigure TightElbow @14:39:20.90 SE.seek 14.08 Pucks below Top Plate @14:39:21.44 Forearm.force -20 @14:39:21.49 Forearm.seek 2 + 326 counts @14:39:21.81 Forearm.reconfigure TightForearm @14:39:21.86 Forearm.seek 2 + 700 counts @14:39:22.16 SE.seek 15.08 Pucks below Top Plate @14:39:22.92 Forearm.reconfigure LooseForearm @14:39:22.97 Forearm.seek :retract @14:39:23.89 Elbow.seek CC @14:39:25.64 Elbow.reconfigure LooseElbow @14:39:25.74 Forearm.seek CC @14:39:26.71 Hand.close @14:39:27.37 CC.seek :open @14:39:31.89 Forearm.seek :retract @14:39:32.86 Elbow.reconfigure TightElbow @14:39:32.91 Elbow.seek 2 @14:39:34.81 Elbow.reconfigure LimpElbow @14:39:34.87 Forearm.seek 2 @14:39:35.87 Elbow.hold @14:39:35.94 Elbow.reconfigure TightElbow @14:39:35.99 Hand.open @14:39:36.74 Forearm.force -20 @14:39:36.78 Forearm.seek 2 + 344 counts @14:39:37.10 Forearm.reconfigure TightForearm @14:39:37.15 Forearm.seek 2 + 700 counts @14:39:37.58 SE.seek :down @14:39:38.72
Forearm.reconfigure LooseForearm @14:39:38.77 Forearm.seek :retract @14:39:38.92 SE.coast @14:39:39.70
SC.coast @14:39:39.73 PSV.dial :air @14:39:40.87 PS.seek 0.499ml @14:39:46.80 delay 3 seconds @14:39:49.81 PSV.dial :mixloop @14:39:50.82 PRV.dial :air @14:39:51.32 delay 3 seconds @14:39:54.33 PRV.dial CBV @14:39:54.83 CBV.dial AIV @14:39:55.34 PRV.dial between CBV and air @14:39:55.69 PRV.dial :air @14:39:56.09 PS.reconfigure PSslow @14:39:56.14 PS.seek 0.599ml @14:39:58.61 PRV.dial :waste2 @14:39:59.41 PS.reconfigure PSconfig @14:39:59.46 PS.seek :empty @14:40:06.46 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:40:06.53 Hand.open @14:40:06.86 Elbow.seek FlushPuck::Garage @14:40:08.82 Elbow.reconfigure LooseElbow @14:40:08.93 Forearm.seek :garage @14:40:09.94 Hand.close @14:40:10.74 Forearm.seek :retract @14:40:11.78 Elbow.reconfigure TightElbow @14:40:11.83 Elbow.seek CC @14:40:14.75 Elbow.reconfigure LooseElbow @14:40:14.86 Forearm.reconfigure TightForearm @14:40:14.91 Forearm.seek CC @14:40:16.17 CC.seek :closed @14:40:20.45 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.10 @14:40:20.52 Hand.open @14:40:21.18 Forearm.reconfigure LooseForearm @14:40:21.22 Forearm.seek :retract @14:40:22.32 Clearing PML for modified lysate @14:40:22.33 CTV.dial :air,avoiding: :intake @14:40:22.34 PRV.dial :waste2 @14:40:22.35 PTV.dial :PRV,avoiding: :puck @14:40:23.15 delay 2 seconds @14:40:25.17 PTV.dial :PRVmixing,avoiding: :puck @14:40:25.96 delay 2 seconds @14:40:27.98 PSV.dial :PML @14:40:28.00 delay 2 seconds @14:40:30.02 PRV.dial :air @14:40:30.82 delay 2 seconds @14:40:32.90 PS.seek 1.499ml @14:40:48.93 delay 5 seconds @14:40:53.96 PS.seek 2.5ml @14:41:04.89 PSV.dial :air @14:41:05.38 PS.seek 0ml @14:41:31.52 mixing lysate with speDil @14:41:31.53 PSV.dial :PML @14:41:32.00 CBV.dial :PRV @14:41:32.02 PRV.dial :air @14:41:32.03 delay 2 seconds @14:41:34.05 PRV.dial :speDil @14:41:34.55 delay 2 seconds @14:41:36.64 PS.reconfigure PSslow1 @14:41:36.69 PS.seek 0.225ml @14:42:00.63 delay 5 seconds @14:42:05.65 PRV.dial CBV @14:42:06.49 delay 2 seconds @14:42:08.57 PS.seek 0.475ml @14:42:34.27 delay 5 seconds @14:42:39.29 PRV.dial :air @14:42:39.80 delay 2 seconds @14:42:41.83 PS.seek 0.965ml @14:43:31.77 delay 10 seconds @14:43:41.80 PRV.dial CBV @14:43:42.29 CTV.dial :air,avoiding: :intake @14:43:42.30 PSV.dial :air @14:43:42.79 PS.reconfigure PSconfig @14:43:42.84 PS.seek 2.5ml @14:43:59.21 delay 3 seconds @14:44:02.23 PSV.dial PTV @14:44:03.01 PTV.dial :PRV,avoiding: :puck @14:44:03.79 PS.seek 0ml @14:44:29.92 PSV.dial :air @14:44:30.70 PS.seek 2.5ml @14:44:56.87 delay 3 seconds @14:44:59.88 PSV.dial PTV @14:45:00.67 PTV.dial :PRV,avoiding: :puck @14:45:00.69 PS.seek 0ml @14:45:26.84 Pushing modified lysate into AIV Injection Loop @14:45:26.85 CBV.dial AIV @14:45:26.86 AIV.dial :bypass @14:45:26.87 PRV.dial CBV @14:45:26.89 PSV.dial :air @14:45:27.67 PS.seek 2.5ml @14:45:53.84 delay 3 seconds @14:45:56.85 PSV.dial :PML @14:45:57.34 PTV.dial :PRVmixing,avoiding: :puck @14:45:58.19 PS.reconfigure PSslow1 @14:45:58.24 PS.seek 1.74ml @14:47:15.84 delay 5 seconds @14:47:20.86 AIV.dial :sample @14:47:21.46 AWV.select :waste @14:47:21.64 PS.reconfigure PSslow2 @14:47:21.69 PS.seek 1.22ml @14:49:09.97 delay 15 seconds @14:49:24.99 AIV.dial :bypass @14:49:25.58 PS.reconfigure PSconfig @14:49:25.63 PS.seek 0ml @14:49:38.77 PSV.dial :air @14:49:39.26 PS.seek 2.5ml @14:50:05.39 PSV.dial :PML @14:50:05.87 PS.seek 0ml @14:50:32.08 AIV.dial :coilToMFB @14:50:32.10 AWV.select :air @14:50:32.27 Extracting Nucleic Acid from AIV @14:50:32.29
Beginning samplePassD @14:50:32.34 SPE.hold 2:46:40,55C @14:50:32.41 ASV.dial :SPEdelivery @14:50:32.50
AWV.select :air @14:50:32.51 AIV.dial :bypass @14:50:32.52 PSV.dial PSR @14:50:32.84 delay 20 seconds @14:50:33.70
Pulling 1.5ml of PSR.killMFB @14:50:33.71 PSR.select :killMFB @14:50:33.84 PS.seek 1.5ml @14:50:49.90 delay 5 seconds @14:50:52.87 primeLysateINT @14:50:52.88 AWV.select :air @14:50:52.89 AIV.dial :coilToMFB @14:50:52.90 ATV.dial AIV @14:50:54.62 AS.reconfigure ASslow1 @14:50:54.67 AS.seek 0.299ml @14:50:54.91
PSV.dial :waste1 @14:50:55.43 PS.seek 0ml @14:51:11.43 PSV.dial PSR @14:51:11.94 PSR.select :air @14:51:12.07 PS.seek 2.5ml @14:51:38.19 PRV.dial CBV @14:51:38.22
CBV.dial PRV @14:51:38.23 PSV.dial :mixloop @14:51:39.89 CTV.dial :air,avoiding: :intake @14:51:39.90 PTV.dial :PRVmixing @14:51:39.92 PS.seek 0ml @14:51:55.32 delay 10 seconds @14:52:05.34 ATV.dial :air2 @14:52:06.10
PSV.dial PSR @14:52:06.24 AS.seek 0.329ml @14:52:07.29
Pulling 1.5ml of PSR.flush @14:52:07.30 PSR.select :flush @14:52:07.43 PS.seek 1.5ml @14:52:12.53 ATV.dial :waste2 @14:52:13.10 AS.reconfigure ASnorm @14:52:13.15 AS.seek :empty @14:52:23.56
delay 5 seconds @14:52:28.59 PSV.dial :waste1 @14:52:29.10 PS.seek 0ml @14:52:30.16 Lysate primed! @14:52:30.17 AFV.select :holdingCoil @14:52:30.18 ATV.dial :air2 @14:52:30.81 AS.reconfigure ASslow1 @14:52:30.86 AS.seek 0.069ml @14:52:45.08
PSV.dial PSR @14:52:45.27 delay 3 seconds @14:52:45.69
PSR.select :air @14:52:45.83 PS.seek 2.5ml @14:52:48.29 ATV.dial :AIV @14:52:49.14 AS.seek 0.469ml @14:53:11.94 PRV.dial CBV @14:53:11.97
CBV.dial PRV @14:53:11.98 PSV.dial :mixloop @14:53:13.12 CTV.dial :air,avoiding: :intake @14:53:13.13 PTV.dial :PRVmixing @14:53:13.15 PS.seek 0ml @14:53:39.49 PSV.dial PSR @14:53:40.79 Pulling 1.5ml of PSR.flush @14:53:40.80 PSR.select :flush @14:53:40.93 PS.seek 1.5ml @14:53:57.00 delay 5 seconds @14:54:02.02 PSV.dial :waste1 @14:54:02.55 PS.seek 0ml @14:54:09.93 delay 5 seconds @14:54:15.00 Solid Phase Extraction Heater at 48C @14:54:15.02 ATV.dial ASV @14:54:15.39 ASV.dial :SPEdelivery @14:54:15.48 AS.seek 0.041ml @14:54:18.49
PSV.dial PSR @14:54:18.98 PSR.select :air @14:54:19.11 PS.seek 2.5ml @14:54:45.19 PRV.dial CBV @14:54:45.22
CBV.dial PRV @14:54:45.24 PSV.dial :mixloop @14:54:46.34 CTV.dial :air,avoiding: :intake @14:54:46.35 PTV.dial :PRVmixing @14:54:46.37 PS.seek 0ml @14:55:12.55 PSV.dial PSR @14:55:13.72 Pulling 1.5ml of PSR.flush @14:55:13.73 PSR.select :flush @14:55:13.86 PS.seek 1.5ml @14:55:29.91 delay 5 seconds @14:55:34.93 PSV.dial :waste1 @14:55:35.44 PS.seek 0ml @14:55:41.84 delay 10 seconds @14:55:51.43
PSV.dial PSR @14:55:51.87 rinseLysateLineINT @14:55:51.88 AFV.select :carrierSolution @14:55:51.99 delay 2 seconds @14:55:52.06
PSR.select :air @14:55:52.19 PS.seek 2.5ml @14:55:54.02 AS.reconfigure ASconfig @14:55:54.07 AS.seek 1ml @14:56:18.38 PRV.dial CBV @14:56:18.40
CBV.dial PRV @14:56:18.43 PSV.dial :mixloop @14:56:19.53 CTV.dial :air,avoiding: :intake @14:56:19.54 PTV.dial :PRVmixing @14:56:19.56 PS.reconfigure PSslow @14:56:19.61 PS.seek 1.2ml @14:56:46.48 PSV.dial :waste1 @14:56:47.88 PS.reconfigure PSconfig @14:56:47.93 PS.seek 0ml @14:56:49.48 delay 1 second @14:56:50.49 AFV.select :holdingCoil @14:56:50.60 delay 1 second @14:56:51.61 ATV.dial AIV @14:56:52.24 AIV.dial :coilToMFB @14:56:52.25 AWV.select :waste @14:56:52.36 AS.reconfigure ASslow0 @14:56:52.41 AS.seek 0ml @14:57:00.84
PSV.dial :air @14:57:01.93 PS.seek 2.5ml @14:57:28.02 PSV.dial :mixloop @14:57:28.49 PS.reconfigure PSslow @14:57:28.54 PS.seek 0ml @14:58:19.87 PSV.dial :air @14:58:20.36 PS.reconfigure PSconfig @14:58:20.41 PS.seek 2.5ml @14:58:33.31 ATV.dial :air2 @14:58:33.97 AS.reconfigure ASslow1 @14:58:34.02 AS.seek 0.05ml @14:58:44.36 ATV.dial :AIV @14:58:45.17 AS.seek 0.01ml @14:58:46.52
PSV.dial :mixloop @14:58:47.00 PS.reconfigure PSslow @14:58:47.05 PS.seek 0ml @14:58:53.49 ATV.dial :waste @14:58:55.03 AS.seek :empty @14:58:57.51 ethanol wash @14:58:57.52 ATV.dial :air2 @14:58:58.73 AS.reconfigure ASslow2 @14:58:58.78 AS.seek 0.009ml @14:59:09.23 delay 3 seconds @14:59:12.26 Pulling 0.4ml of Ethanol Wash @14:59:12.27 ATV.dial :ethanol @14:59:13.15 AS.reconfigure ASslow1 @14:59:13.21 AS.seek 0.409ml @14:59:38.25
Cleaning Collection Syringe @14:59:38.26 CSV.dial CSR @14:59:38.79 Pulling 4ml of CSR.flush @14:59:38.80 CSR.select :flush @14:59:38.93 CS.seek 4ml @14:59:50.19 delay 5 seconds @14:59:55.21 CSR.select :air @14:59:55.35 CS.seek 10ml @15:00:11.69 CSV.dial CTV @15:00:11.72
CTV.dial CSV @15:00:13.22 CBV.dial :waste1 @15:00:13.75 CS.seek 0ml @15:00:33.98 delay 3 seconds @15:00:37.00 ATV.dial :air1 @15:00:37.40 delay 3 seconds @15:00:40.30
CSV.dial CSR @15:00:40.47 AS.seek 0.434ml @15:00:40.85
Pulling 4ml of CSR.flush @15:00:40.86 CSR.select :flush @15:00:40.99 CS.seek 4ml @15:00:45.79 delay 3 seconds @15:00:48.81 ATV.dial :ethanol @15:00:49.41 AS.seek 0.419ml @15:00:52.15
delay 5 seconds @15:00:52.67 delay 3 seconds @15:00:55.69 ATV.dial ASV @15:00:56.96 AS.seek 0.092ml @15:00:57.16
CSR.select :air @15:00:57.30 CS.seek 10ml @15:01:13.56 CSV.dial CTV @15:01:13.59
CTV.dial CSV @15:01:14.09 CBV.dial :waste1 @15:01:14.11 CS.seek 0ml @15:01:40.65 Garaging FlushPuck in Collection Clamp @15:01:40.73 Elbow.reconfigure TightElbow @15:01:40.78 Elbow.seek CC @15:01:41.11 Elbow.reconfigure LooseElbow @15:01:41.77 Forearm.seek CC @15:01:42.74 Hand.close @15:01:43.39 CC.seek :open @15:01:47.75 Forearm.seek :retract @15:01:48.72 Elbow.reconfigure TightElbow @15:01:48.77 Elbow.seek :garage @15:01:51.83 Elbow.reconfigure LooseElbow @15:01:51.96 Forearm.reconfigure TightForearm @15:01:52.01 Forearm.seek :garage @15:01:53.16 Hand.open @15:01:53.84 Forearm.reconfigure LooseForearm @15:01:53.89 Forearm.seek :retract @15:01:54.87 Whole Cell Archive sampling 1500ml @15:01:54.89 Loading wcr puck from tube 3 into Collection Clamp @15:01:55.16 SC.reconfigure SCtight @15:01:55.21 SC.seek 3 @15:01:57.39 SC.reconfigure SCloose @15:01:57.62 Elbow.reconfigure TightElbow @15:01:57.67 Elbow.seek 3 @15:01:59.69 Forearm.reconfigure TightForearm @15:01:59.74 Forearm.seek 3 + 700 counts @15:02:00.79 SE.reconfigure SEpick @15:02:00.87 SE.seek beamBreak @15:02:02.95 delay 3 seconds @15:02:03.53 SE.configure SEconfig @15:02:03.71 Elbow.seek 3 @15:02:03.92 Forearm.seek 3 + 300 counts @15:02:04.22 Forearm.reconfigure LooseForearm @15:02:04.27 Hand.close @15:02:04.92 SE.seek :down @15:02:05.16 Forearm.seek :retract @15:02:05.96 ATV.dial :waste2 @15:02:06.14 Elbow.seek CC @15:02:06.68 AS.reconfigure ASconfig @15:02:06.73 AS.seek :empty @15:02:07.27 SE.coast @15:02:07.82 Elbow.reconfigure LooseElbow @15:02:07.93 Forearm.reconfigure TightForearm @15:02:07.98 Forearm.seek CC @15:02:09.31 CC.seek :closed @15:02:12.40 ATV.dial :air2 @15:02:12.78 AS.reconfigure ASslow1 @15:02:13.63 AS.seek 1ml @15:02:13.74 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.22 @15:02:13.77 Hand.open @15:02:14.46 Forearm.reconfigure LooseForearm @15:02:14.51 Forearm.seek :retract @15:02:15.48 SC.coast @15:02:15.52 collectSample 1500ml w/bubble point of 55psi @15:02:15.54 Priming 7ml @15:02:15.55 Intake.select :close @15:02:15.56 Exhaust.select :close @15:02:15.57 SS.hold @15:02:15.80 Calibrating pressure sensors @15:02:15.81 CTV.dial :air @15:02:17.22 SSV.dial :spit,avoiding: :exhaust @15:02:17.24 SS.reconfigure SSfast @15:02:17.29 SS.seek 5ml @15:02:19.96 SSV.dial CBV @15:02:19.99 CBV.dial SSV @15:02:21.52 delay 3.8 seconds @15:02:25.46 SSV.dial :spit @15:02:26.88 SS.seek 0ml @15:02:29.54 SSV.dial CBV @15:02:30.98 CTV.dial :intake @15:02:31.80 SSV.dial CBV @15:02:31.83 CBV.dial SSV @15:02:31.84 Allowing 40s for 7.0ml of remaining 7.0ml @15:02:31.87 Intake.select :open @15:02:34.04 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:02:34.07 SS.reconfigure SSinhale.with maxSpeed: 150 @15:02:34.11 SS.seek 7ml @15:02:37.62 Vacuum too high -- waiting up to 40s @15:02:40.00 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:02:40.02 SS.reconfigure SSinhale.with maxSpeed: 135 @15:02:40.07 SS.seek 7ml @15:02:43.69 Stroke completed -- waiting for pressure to equalize ... @15:02:48.85 Intake.select :close @15:02:49.01 SSV.dial :exhaust @15:02:49.84 Exhaust.select :open @15:02:49.95 SS.reconfigure SSexhale @15:02:50.00 SS.seek 0ml @15:02:53.83 Exhaust.select :close @15:02:53.94 Sampling 1500.0ml @15:02:53.96 Intake.select :close @15:02:53.97 Exhaust.select :close @15:02:53.98 SS.reconfigure SSconfig @15:02:54.03 SS.hold @15:02:54.25 CTV.dial :intake @15:02:54.27 SSV.dial CBV @15:02:54.30 CBV.dial SSV @15:02:55.15 Allowing 130s for 25.0ml of remaining 1500.0ml @15:02:55.16 Intake.select :open @15:02:57.91 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:02:57.93 SS.reconfigure SSinhale.with maxSpeed: 135 @15:02:57.98 SS.seek 25ml @15:03:01.55 Vacuum too high -- waiting up to 40s @15:03:03.16 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:03:03.18 SS.reconfigure SSinhale.with maxSpeed: 122 @15:03:03.23 SS.seek 25ml @15:03:05.80 Vacuum too high -- waiting up to 40s @15:03:07.44 SS.reconfigure SSinhale.with maxSpeed: 110 @15:03:07.50 SS.seek 25ml @15:03:10.18 Vacuum too high -- waiting up to 40s @15:03:11.79 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:03:11.81 SS.reconfigure SSinhale.with maxSpeed: 99 @15:03:11.86 SS.seek 25ml @15:03:14.49 Vacuum too high -- waiting up to 40s @15:03:15.88 SS.reconfigure SSinhale.with maxSpeed: 89 @15:03:15.93 SS.seek 25ml @15:03:18.46 Vacuum too high -- waiting up to 40s @15:03:19.84 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:03:19.86 SS.reconfigure SSinhale.with maxSpeed: 80 @15:03:19.91 SS.seek 25ml @15:03:22.71 Vacuum too high -- waiting up to 40s @15:03:24.08 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:03:24.10 SS.reconfigure SSinhale.with maxSpeed: 72 @15:03:24.15 SS.seek 25ml @15:03:27.18 Vacuum too high -- waiting up to 40s @15:03:28.28 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:03:28.30 SS.reconfigure SSinhale.with maxSpeed: 65 @15:03:28.35 SS.seek 25ml @15:03:33.43 Vacuum too high -- waiting up to 40s @15:03:34.53 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:03:34.55 SS.reconfigure SSinhale.with maxSpeed: 59 @15:03:34.60 SS.seek 25ml @15:03:38.80 Stroke completed -- waiting for pressure to equalize ... @15:03:42.04 Intake.select :close @15:03:42.21 SSV.dial :exhaust @15:03:43.04 Exhaust.select :open @15:03:43.15 SS.reconfigure SSexhale @15:03:43.20 SS.seek 0ml @15:03:52.31 Exhaust.select :close @15:03:52.43 SSV.dial CBV @15:03:52.46 CBV.dial SSV @15:03:53.31 Allowing 130s for 25.0ml of remaining 1475.0ml @15:03:53.32 Intake.select :open @15:03:55.50 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:03:55.53 SS.reconfigure SSinhale.with maxSpeed: 59 @15:03:55.57 SS.seek 25ml @15:04:01.80 Vacuum too high -- waiting up to 40s @15:04:02.93 SS.reconfigure SSinhale.with maxSpeed: 53 @15:04:02.98 SS.seek 25ml @15:04:11.30 Vacuum too high -- waiting up to 40s @15:04:12.15 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:04:12.17 SS.reconfigure SSinhale.with maxSpeed: 48 @15:04:12.22 SS.seek 25ml @15:04:33.74 Stroke completed -- waiting for pressure to equalize ... @15:04:36.97 Intake.select :close @15:04:37.14 SSV.dial :exhaust @15:04:37.96 Exhaust.select :open @15:04:38.07 SS.reconfigure SSexhale @15:04:38.12 SS.seek 0ml @15:04:47.24 Exhaust.select :close @15:04:47.36 SSV.dial CBV @15:04:47.39 CBV.dial SSV @15:04:48.23 Allowing 130s for 25.0ml of remaining 1450.0ml @15:04:48.24 Intake.select :open @15:04:50.42 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:04:50.44 SS.reconfigure SSinhale.with maxSpeed: 48 @15:04:50.49 SS.seek 25ml @15:05:27.12 Vacuum too high -- waiting up to 40s @15:05:27.97 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:05:27.99 SS.reconfigure SSinhale.with maxSpeed: 43 @15:05:28.04 SS.seek 25ml @15:05:28.31 Stroke completed -- waiting for pressure to equalize ... @15:05:30.87 Intake.select :close @15:05:31.03 SSV.dial :exhaust @15:05:31.86 Exhaust.select :open @15:05:31.97 SS.reconfigure SSexhale @15:05:32.03 SS.seek 0ml @15:05:35.28 delay 5 seconds @15:05:40.30 ATV.dial ASV @15:05:40.70 Exhaust.select :close @15:05:40.82 SSV.dial CBV @15:05:40.85 CBV.dial SSV @15:05:41.24 ASV.dial :SPEdelivery @15:05:41.27 AS.seek 0.02ml @15:05:41.70 Allowing 130s for 25.0ml of remaining 1425.0ml @15:05:41.71 Intake.select :open @15:05:43.90 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:05:43.92 SS.reconfigure SSinhale.with maxSpeed: 43 @15:05:43.97 SS.seek 25ml @15:06:25.92 Stroke completed -- waiting for pressure to equalize ... @15:06:28.53 Intake.select :close @15:06:28.69 SSV.dial :exhaust @15:06:29.51 Exhaust.select :open @15:06:29.63 SS.reconfigure SSexhale @15:06:29.68 SS.seek 0ml @15:06:38.61 Exhaust.select :close @15:06:38.73 SSV.dial CBV @15:06:38.76 CBV.dial SSV @15:06:39.62 Allowing 130s for 25.0ml of remaining 1400.0ml @15:06:39.63 Intake.select :open @15:06:41.82 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:06:41.84 SS.reconfigure SSinhale.with maxSpeed: 43 @15:06:41.89 SS.seek 25ml @15:07:23.59 Stroke completed -- waiting for pressure to equalize ... @15:07:26.83 Intake.select :close @15:07:26.99 SSV.dial :exhaust @15:07:27.81 Exhaust.select :open @15:07:27.92 SS.reconfigure SSexhale @15:07:27.97 SS.seek 0ml @15:07:36.74 Exhaust.select :close @15:07:36.86 SSV.dial CBV @15:07:36.89 CBV.dial SSV @15:07:37.75 Allowing 130s for 25.0ml of remaining 1375.0ml @15:07:37.76 Intake.select :open @15:07:39.94 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:07:39.96 SS.reconfigure SSinhale.with maxSpeed: 43 @15:07:40.01 SS.seek 25ml @15:08:21.78 Stroke completed -- waiting for pressure to equalize ... @15:08:25.01 Intake.select :close @15:08:25.18 SSV.dial :exhaust @15:08:26.00 Exhaust.select :open @15:08:26.12 SS.reconfigure SSexhale @15:08:26.17 SS.seek 0ml @15:08:35.16 Exhaust.select :close @15:08:35.28 SSV.dial CBV @15:08:35.31 CBV.dial SSV @15:08:36.15 Allowing 130s for 25.0ml of remaining 1350.0ml @15:08:36.16 Intake.select :open @15:08:38.34 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:08:38.36 SS.reconfigure SSinhale.with maxSpeed: 43 @15:08:38.41 SS.seek 25ml @15:08:58.64 delay 5 seconds @15:09:03.66 ATV.dial :air2 @15:09:05.24 AS.seek 0.52ml @15:09:19.75 Stroke completed -- waiting for pressure to equalize ... @15:09:22.99 Intake.select :close @15:09:23.15 SSV.dial :exhaust @15:09:23.98 Exhaust.select :open @15:09:24.09 SS.reconfigure SSexhale @15:09:24.15 SS.seek 0ml @15:09:33.22 Exhaust.select :close @15:09:33.34 SSV.dial CBV @15:09:33.37 CBV.dial SSV @15:09:34.23 Allowing 130s for 25.0ml of remaining 1325.0ml @15:09:34.24 Intake.select :open @15:09:36.42 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:09:36.45 SS.reconfigure SSinhale.with maxSpeed: 43 @15:09:36.50 SS.seek 25ml @15:10:17.78 Stroke completed -- waiting for pressure to equalize ... @15:10:21.03 Intake.select :close @15:10:21.19 SSV.dial :exhaust @15:10:22.01 Exhaust.select :open @15:10:22.12 SS.reconfigure SSexhale @15:10:22.17 SS.seek 0ml @15:10:30.99 Exhaust.select :close @15:10:31.11 SSV.dial CBV @15:10:31.14 CBV.dial SSV @15:10:32.00 Allowing 130s for 25.0ml of remaining 1300.0ml @15:10:32.01 Intake.select :open @15:10:34.20 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:10:34.22 SS.reconfigure SSinhale.with maxSpeed: 43 @15:10:34.26 SS.seek 25ml @15:10:46.11 delay 5 seconds @15:10:51.15 ATV.dial ASV @15:10:52.11 ASV.dial :wasteR @15:10:52.57 AS.seek 0.3ml @15:11:15.95 Stroke completed -- waiting for pressure to equalize ... @15:11:19.79 Intake.select :close @15:11:19.96 SSV.dial :exhaust @15:11:20.77 Exhaust.select :open @15:11:20.89 SS.reconfigure SSexhale @15:11:20.94 SS.seek 0ml @15:11:29.78 Exhaust.select :close @15:11:29.90 SSV.dial CBV @15:11:29.93 CBV.dial SSV @15:11:30.78 Allowing 130s for 25.0ml of remaining 1275.0ml @15:11:30.79 Intake.select :open @15:11:32.97 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:11:33.00 SS.reconfigure SSinhale.with maxSpeed: 43 @15:11:33.04 SS.seek 25ml @15:11:37.05 delay 20 seconds @15:11:57.09 AS.seek 0.07ml @15:12:14.55 Stroke completed -- waiting for pressure to equalize ... @15:12:18.87 Intake.select :close @15:12:19.13 SSV.dial :exhaust @15:12:19.96 Exhaust.select :open @15:12:20.08 SS.reconfigure SSexhale @15:12:20.14 SS.seek 0ml @15:12:28.91 Exhaust.select :close @15:12:29.03 SSV.dial CBV @15:12:29.06 CBV.dial SSV @15:12:29.92 Allowing 130s for 25.0ml of remaining 1250.0ml @15:12:29.94 Intake.select :open @15:12:32.11 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:12:32.13 SS.reconfigure SSinhale.with maxSpeed: 43 @15:12:32.18 SS.seek 25ml @15:12:43.62 delay 20 seconds @15:13:03.64 ATV.dial :waste2 @15:13:04.37 AS.reconfigure ASconfig @15:13:04.42 AS.seek :empty @15:13:08.82 Elution @15:13:08.83 ATV.dial :air1 @15:13:09.93 AS.reconfigure ASslow1 @15:13:09.98 AS.seek 0.049ml @15:13:13.93 Stroke completed -- waiting for pressure to equalize ... @15:13:17.83 Intake.select :close @15:13:17.99 SSV.dial :exhaust @15:13:18.80 Exhaust.select :open @15:13:18.92 SS.reconfigure SSexhale @15:13:18.99 SS.seek 0ml @15:13:20.37 delay 3 seconds @15:13:23.38 ATV.dial :ElutionWater @15:13:24.05 AS.seek 0.109ml @15:13:28.14 Exhaust.select :close @15:13:28.26 SSV.dial CBV @15:13:28.29 CBV.dial SSV @15:13:29.15 Allowing 130s for 25.0ml of remaining 1225.0ml @15:13:29.16 Intake.select :open @15:13:31.34 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:13:31.36 SS.reconfigure SSinhale.with maxSpeed: 43 @15:13:31.41 SS.seek 25ml @15:13:36.39 delay 3 seconds @15:13:39.42 ATV.dial ASV,via: :air2 @15:13:41.43 ASV.dial :SPEelution @15:13:42.00 AFV.select :carrierSolution @15:13:42.16 AS.reconfigure ASnorm @15:13:42.21 AS.seek 0.759ml @15:14:13.02 Stroke completed -- waiting for pressure to equalize ... @15:14:15.29 delay 3 seconds @15:14:17.53 Intake.select :close @15:14:17.69 SSV.dial :exhaust @15:14:18.31 AFV.select :holdingCoil @15:14:18.50 AS.reconfigure ASslow1 @15:14:18.57 AS.seek 0.474ml @15:14:18.59 Exhaust.select :open @15:14:18.73 SS.reconfigure SSexhale @15:14:18.78 SS.seek 0ml @15:14:27.88 Exhaust.select :close @15:14:28.00 SSV.dial CBV @15:14:28.03 CBV.dial SSV @15:14:28.87 Allowing 130s for 25.0ml of remaining 1200.0ml @15:14:28.88 Intake.select :open @15:14:31.06 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:14:31.08 SS.reconfigure SSinhale.with maxSpeed: 43 @15:14:31.13 SS.seek 25ml @15:15:12.41 Stroke completed -- waiting for pressure to equalize ... @15:15:16.22 delay 5 seconds @15:15:16.84 Intake.select :close @15:15:17.01 SSV.dial :exhaust @15:15:17.84 Exhaust.select :open @15:15:17.95 SS.reconfigure SSexhale @15:15:18.01 SS.seek 0ml @15:15:21.31 AS.reconfigure ASslow2 @15:15:21.36 AS.seek 0.439ml @15:15:27.16 Exhaust.select :close @15:15:27.28 SSV.dial CBV @15:15:27.31 CBV.dial SSV @15:15:28.17 Allowing 130s for 25.0ml of remaining 1175.0ml @15:15:28.18 Intake.select :open @15:15:30.37 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:15:30.39 SS.reconfigure SSinhale.with maxSpeed: 43 @15:15:30.44 SS.seek 25ml @15:15:56.75 delay 1:00 @15:16:08.52 Vacuum too high -- waiting up to 40s @15:16:09.89 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:16:09.91 SS.reconfigure SSinhale.with maxSpeed: 39 @15:16:09.96 SS.seek 25ml @15:16:14.06 Stroke completed -- waiting for pressure to equalize ... @15:16:17.29 Intake.select :close @15:16:17.46 SSV.dial :exhaust @15:16:18.28 Exhaust.select :open @15:16:18.39 SS.reconfigure SSexhale @15:16:18.44 SS.seek 0ml @15:16:27.61 Exhaust.select :close @15:16:27.73 SSV.dial CBV @15:16:27.76 CBV.dial SSV @15:16:28.61 Allowing 130s for 25.0ml of remaining 1150.0ml @15:16:28.62 Intake.select :open @15:16:30.80 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:16:30.83 SS.reconfigure SSinhale.with maxSpeed: 39 @15:16:30.87 SS.seek 25ml @15:16:56.78 pushing eluate to holding coil @15:16:56.85 AS.seek 0.279ml @15:17:16.39 Stroke completed -- waiting for pressure to equalize ... @15:17:20.27 Intake.select :close @15:17:20.44 SSV.dial :exhaust @15:17:21.26 Exhaust.select :open @15:17:21.37 SS.reconfigure SSexhale @15:17:21.43 SS.seek 0ml @15:17:30.61 Exhaust.select :close @15:17:30.73 SSV.dial CBV @15:17:30.76 CBV.dial SSV @15:17:31.62 Allowing 130s for 25.0ml of remaining 1125.0ml @15:17:31.63 Intake.select :open @15:17:33.81 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:17:33.84 SS.reconfigure SSinhale.with maxSpeed: 39 @15:17:33.90 SS.seek 25ml @15:18:18.99 Stroke completed -- waiting for pressure to equalize ... @15:18:22.81 Intake.select :close @15:18:22.97 SSV.dial :exhaust @15:18:23.79 Exhaust.select :open @15:18:23.91 SS.reconfigure SSexhale @15:18:23.97 SS.seek 0ml @15:18:33.53 Exhaust.select :close @15:18:33.65 SSV.dial CBV @15:18:33.68 CBV.dial SSV @15:18:34.53 Allowing 130s for 25.0ml of remaining 1100.0ml @15:18:34.54 Intake.select :open @15:18:36.73 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:18:36.75 SS.reconfigure SSinhale.with maxSpeed: 39 @15:18:36.80 SS.seek 25ml @15:19:22.41 Stroke completed -- waiting for pressure to equalize ... @15:19:26.30 Intake.select :close @15:19:26.46 SSV.dial :exhaust @15:19:27.27 Exhaust.select :open @15:19:27.39 SS.reconfigure SSexhale @15:19:27.46 SS.seek 0ml @15:19:36.84 Exhaust.select :close @15:19:36.96 SSV.dial CBV @15:19:36.99 CBV.dial SSV @15:19:37.82 Allowing 130s for 25.0ml of remaining 1075.0ml @15:19:37.83 Intake.select :open @15:19:37.91 delay 30 seconds @15:19:40.02 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:19:40.04 SS.reconfigure SSinhale.with maxSpeed: 39 @15:19:40.09 SS.seek 25ml @15:20:07.94 ATV.dial :waste,via: :air3 @15:20:09.58 AS.reconfigure ASconfig @15:20:09.63 AS.seek :empty @15:20:25.10 Stroke completed -- waiting for pressure to equalize ... @15:20:26.01 Parking Bleach in LysateSource line and SPEcolumn @15:20:26.03 ATV.dial :air3 @15:20:26.82 AS.reconfigure ASslow1 @15:20:26.87 AS.seek 0.029ml @15:20:29.62 Intake.select :close @15:20:29.78 SSV.dial :exhaust @15:20:30.62 Exhaust.select :open @15:20:30.73 SS.reconfigure SSexhale @15:20:30.79 SS.seek 0ml @15:20:33.27 ATV.dial :ElutionWater @15:20:33.87 AS.seek 0.059ml @15:20:39.63 Exhaust.select :close @15:20:39.75 SSV.dial CBV @15:20:39.78 CBV.dial SSV @15:20:40.21 ATV.dial :air3 @15:20:40.64 Allowing 130s for 25.0ml of remaining 1050.0ml @15:20:40.65 Intake.select :open @15:20:40.79 AS.seek 0.069ml @15:20:42.92 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:20:42.94 SS.reconfigure SSinhale.with maxSpeed: 39 @15:20:42.99 SS.seek 25ml @15:20:43.06 delay 2 seconds @15:20:45.08 ATV.dial :bleach @15:20:46.11 AS.seek 0.769ml @15:21:28.81 Stroke completed -- waiting for pressure to equalize ... @15:21:33.31 Intake.select :close @15:21:33.47 SSV.dial :exhaust @15:21:34.29 Exhaust.select :open @15:21:34.40 SS.reconfigure SSexhale @15:21:34.46 SS.seek 0ml @15:21:43.66 Exhaust.select :close @15:21:43.78 SSV.dial CBV @15:21:43.81 CBV.dial SSV @15:21:44.65 Allowing 130s for 25.0ml of remaining 1025.0ml @15:21:44.66 Intake.select :open @15:21:46.84 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:21:46.86 SS.reconfigure SSinhale.with maxSpeed: 39 @15:21:46.91 SS.seek 25ml @15:22:30.00 Vacuum too high -- waiting up to 40s @15:22:31.36 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:22:31.38 SS.reconfigure SSinhale.with maxSpeed: 35 @15:22:31.43 SS.seek 25ml @15:22:34.17 Stroke completed -- waiting for pressure to equalize ... @15:22:37.42 Intake.select :close @15:22:37.59 SSV.dial :exhaust @15:22:38.41 Exhaust.select :open @15:22:38.53 SS.reconfigure SSexhale @15:22:38.58 SS.seek 0ml @15:22:47.88 Exhaust.select :close @15:22:48.00 SSV.dial CBV @15:22:48.03 CBV.dial SSV @15:22:48.89 Allowing 130s for 25.0ml of remaining 1000.0ml @15:22:48.90 Intake.select :open @15:22:51.09 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:22:51.11 SS.reconfigure SSinhale.with maxSpeed: 35 @15:22:51.15 SS.seek 25ml @15:23:07.14 delay 3 seconds @15:23:10.16 ATV.dial :air3 @15:23:10.71 delay 3 seconds @15:23:13.80 AS.seek 0.794ml @15:23:19.10 delay 3 seconds @15:23:22.11 ATV.dial :bleach @15:23:22.73 AS.seek 0.779ml @15:23:26.06 delay 3 seconds @15:23:29.08 ATV.dial :AIV @15:23:30.30 AS.reconfigure ASconfig @15:23:30.35 AS.seek 0.015ml @15:23:41.49 Stroke completed -- waiting for pressure to equalize ... @15:23:45.35 Intake.select :close @15:23:45.51 SSV.dial :exhaust @15:23:46.33 Exhaust.select :open @15:23:47.01 SS.reconfigure SSexhale @15:23:47.09 SS.seek 0ml @15:23:56.34 Exhaust.select :close @15:23:56.46 SSV.dial CBV @15:23:56.49 CBV.dial SSV @15:23:57.32 Allowing 130s for 25.0ml of remaining 975.0ml @15:23:57.33 Intake.select :open @15:23:59.52 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:23:59.54 SS.reconfigure SSinhale.with maxSpeed: 35 @15:23:59.59 SS.seek 25ml @15:24:14.55 ATV.dial :waste @15:24:15.97 AS.seek :empty @15:24:17.18 ATV.dial :air3 @15:24:17.85 AS.reconfigure ASslow1 @15:24:17.90 AS.seek 0.009ml @15:24:20.22 delay 2 seconds @15:24:22.23 ATV.dial :bleach @15:24:22.70 AS.seek 0.579ml @15:24:49.94 Stroke completed -- waiting for pressure to equalize ... @15:24:54.43 Intake.select :close @15:24:54.60 SSV.dial :exhaust @15:24:55.42 Exhaust.select :open @15:24:55.53 SS.reconfigure SSexhale @15:24:55.58 SS.seek 0ml @15:25:04.75 Exhaust.select :close @15:25:04.87 SSV.dial CBV @15:25:04.90 CBV.dial SSV @15:25:05.75 Allowing 130s for 25.0ml of remaining 950.0ml @15:25:05.76 Intake.select :open @15:25:07.95 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:25:07.97 SS.reconfigure SSinhale.with maxSpeed: 35 @15:25:08.02 SS.seek 25ml @15:25:58.39 Stroke completed -- waiting for pressure to equalize ... @15:26:02.91 Intake.select :close @15:26:03.07 SSV.dial :exhaust @15:26:03.90 Exhaust.select :open @15:26:04.01 SS.reconfigure SSexhale @15:26:04.06 SS.seek 0ml @15:26:13.20 Exhaust.select :close @15:26:13.32 SSV.dial CBV @15:26:13.35 CBV.dial SSV @15:26:14.20 Allowing 130s for 25.0ml of remaining 925.0ml @15:26:14.21 Intake.select :open @15:26:16.39 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:26:16.41 SS.reconfigure SSinhale.with maxSpeed: 35 @15:26:16.46 SS.seek 25ml @15:26:17.67 delay 3 seconds @15:26:20.68 ATV.dial :air3 @15:26:21.21 delay 3 seconds @15:26:24.29 AS.seek 0.604ml @15:26:29.60 delay 3 seconds @15:26:32.62 ATV.dial :bleach @15:26:33.23 AS.seek 0.589ml @15:26:36.49 delay 3 seconds @15:26:39.51 ATV.dial :ASV @15:26:40.49 ASV.dial :wasteR @15:26:41.08 AS.seek 0ml @15:27:06.92 Stroke completed -- waiting for pressure to equalize ... @15:27:12.01 Intake.select :close @15:27:12.18 SSV.dial :exhaust @15:27:13.00 Exhaust.select :open @15:27:13.12 SS.reconfigure SSexhale @15:27:13.18 SS.seek 0ml @15:27:22.47 Exhaust.select :close @15:27:22.59 SSV.dial CBV @15:27:22.62 CBV.dial SSV @15:27:23.46 Allowing 130s for 25.0ml of remaining 900.0ml @15:27:23.47 Intake.select :open @15:27:25.65 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:27:25.68 SS.reconfigure SSinhale.with maxSpeed: 35 @15:27:25.73 SS.seek 25ml @15:28:15.95 Stroke completed -- waiting for pressure to equalize ... @15:28:21.10 Intake.select :close @15:28:21.26 SSV.dial :exhaust @15:28:22.09 Exhaust.select :open @15:28:22.20 SS.reconfigure SSexhale @15:28:22.25 SS.seek 0ml @15:28:31.61 Exhaust.select :close @15:28:31.73 SSV.dial CBV @15:28:31.76 CBV.dial SSV @15:28:32.62 Allowing 130s for 25.0ml of remaining 875.0ml @15:28:32.63 Intake.select :open @15:28:34.81 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:28:34.83 SS.reconfigure SSinhale.with maxSpeed: 35 @15:28:34.88 SS.seek 25ml @15:28:39.82 delay 1:00 @15:29:25.42 Stroke completed -- waiting for pressure to equalize ... @15:29:30.58 Intake.select :close @15:29:30.74 SSV.dial :exhaust @15:29:31.55 Exhaust.select :open @15:29:31.67 SS.reconfigure SSexhale @15:29:31.73 SS.seek 0ml @15:29:39.84 AFV.select :carrierSolution @15:29:39.95 delay 2 seconds @15:29:41.06 Exhaust.select :close @15:29:41.18 SSV.dial CBV @15:29:41.21 CBV.dial SSV @15:29:41.98 AS.reconfigure ASconfig @15:29:42.05 Allowing 130s for 25.0ml of remaining 850.0ml @15:29:42.08 AS.seek 1ml @15:29:42.15 Intake.select :open @15:29:44.41 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:29:44.43 SS.reconfigure SSinhale.with maxSpeed: 35 @15:29:44.48 SS.seek 25ml @15:30:23.49 Vacuum too high -- waiting up to 40s @15:30:25.42 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:30:25.44 SS.reconfigure SSinhale.with maxSpeed: 32 @15:30:25.49 SS.seek 25ml @15:30:38.58 Stroke completed -- waiting for pressure to equalize ... @15:30:39.95 delay 1 second @15:30:40.97 AFV.select :holdingCoil @15:30:41.08 delay 1 second @15:30:42.09 ATV.dial ASV @15:30:42.11 ASV.dial :wasteR @15:30:42.13 AS.reconfigure ASslow1 @15:30:42.18 AS.seek 0ml @15:30:43.18 Intake.select :close @15:30:43.34 SSV.dial :exhaust @15:30:44.17 Exhaust.select :open @15:30:44.28 SS.reconfigure SSexhale @15:30:44.34 SS.seek 0ml @15:30:53.61 Exhaust.select :close @15:30:53.73 SSV.dial CBV @15:30:53.76 CBV.dial SSV @15:30:54.60 Allowing 130s for 25.0ml of remaining 825.0ml @15:30:54.61 Intake.select :open @15:30:56.79 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:30:56.81 SS.reconfigure SSinhale.with maxSpeed: 32 @15:30:56.86 SS.seek 25ml @15:31:51.78 Stroke completed -- waiting for pressure to equalize ... @15:31:56.91 Intake.select :close @15:31:57.07 SSV.dial :exhaust @15:31:57.88 Exhaust.select :open @15:31:58.00 SS.reconfigure SSexhale @15:31:58.05 SS.seek 0ml @15:32:07.16 Exhaust.select :close @15:32:07.28 SSV.dial CBV @15:32:07.31 CBV.dial SSV @15:32:08.17 Allowing 130s for 25.0ml of remaining 800.0ml @15:32:08.18 Intake.select :open @15:32:10.37 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:32:10.39 SS.reconfigure SSinhale.with maxSpeed: 32 @15:32:10.44 SS.seek 25ml @15:33:05.47 Stroke completed -- waiting for pressure to equalize ... @15:33:10.61 Intake.select :close @15:33:10.77 SSV.dial :exhaust @15:33:11.59 Exhaust.select :open @15:33:11.70 SS.reconfigure SSexhale @15:33:11.75 SS.seek 0ml @15:33:20.61 Exhaust.select :close @15:33:20.73 SSV.dial CBV @15:33:20.76 CBV.dial SSV @15:33:21.62 Allowing 130s for 25.0ml of remaining 775.0ml @15:33:21.63 Intake.select :open @15:33:23.81 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:33:23.83 SS.reconfigure SSinhale.with maxSpeed: 32 @15:33:24.46 SS.seek 25ml @15:34:03.58 delay 20 seconds @15:34:19.77 Stroke completed -- waiting for pressure to equalize ... @15:34:23.60 ATV.dial :air3 @15:34:25.06 AS.seek 0.59ml @15:34:25.56 Intake.select :close @15:34:25.73 SSV.dial :exhaust @15:34:26.56 Exhaust.select :open @15:34:26.67 SS.reconfigure SSexhale @15:34:26.73 SS.seek 0ml @15:34:35.61 Exhaust.select :close @15:34:35.73 SSV.dial CBV @15:34:35.76 CBV.dial SSV @15:34:36.62 Allowing 130s for 25.0ml of remaining 750.0ml @15:34:36.63 Intake.select :open @15:34:38.82 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:34:38.84 SS.reconfigure SSinhale.with maxSpeed: 32 @15:34:38.88 SS.seek 25ml @15:35:24.58 Vacuum too high -- waiting up to 40s @15:35:25.95 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:35:25.97 SS.reconfigure SSinhale.with maxSpeed: 29 @15:35:26.02 SS.seek 25ml @15:35:36.81 Stroke completed -- waiting for pressure to equalize ... @15:35:41.95 Intake.select :close @15:35:42.11 SSV.dial :exhaust @15:35:42.92 Exhaust.select :open @15:35:43.04 SS.reconfigure SSexhale @15:35:43.09 SS.seek 0ml @15:35:51.96 Exhaust.select :close @15:35:52.07 SSV.dial CBV @15:35:52.10 CBV.dial SSV @15:35:52.95 Allowing 130s for 25.0ml of remaining 725.0ml @15:35:52.96 Intake.select :open @15:35:55.14 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:35:55.16 SS.reconfigure SSinhale.with maxSpeed: 29 @15:35:55.21 SS.seek 25ml @15:36:24.01 delay 3 seconds @15:36:27.03 ATV.dial :ASV @15:36:28.50 ASV.dial :wasteR @15:36:28.59 AS.seek 0.09ml @15:36:56.11 Stroke completed -- waiting for pressure to equalize ... @15:37:01.24 Intake.select :close @15:37:01.40 SSV.dial :exhaust @15:37:02.23 Exhaust.select :open @15:37:02.34 SS.reconfigure SSexhale @15:37:02.40 SS.seek 0ml @15:37:11.42 Exhaust.select :close @15:37:11.54 SSV.dial CBV @15:37:11.57 CBV.dial SSV @15:37:12.43 Allowing 130s for 25.0ml of remaining 700.0ml @15:37:12.44 Intake.select :open @15:37:14.62 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:37:14.64 SS.reconfigure SSinhale.with maxSpeed: 29 @15:37:14.69 SS.seek 25ml @15:38:09.43 delay 30 seconds @15:38:15.59 Stroke completed -- waiting for pressure to equalize ... @15:38:21.37 Intake.select :close @15:38:21.53 SSV.dial :exhaust @15:38:22.34 Exhaust.select :open @15:38:22.46 SS.reconfigure SSexhale @15:38:22.51 SS.seek 0ml @15:38:31.98 Exhaust.select :close @15:38:32.10 SSV.dial CBV @15:38:32.13 CBV.dial SSV @15:38:32.98 Allowing 130s for 25.0ml of remaining 675.0ml @15:38:32.99 Intake.select :open @15:38:35.17 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:38:35.19 SS.reconfigure SSinhale.with maxSpeed: 29 @15:38:35.24 SS.seek 25ml @15:38:39.44 ATV.dial :extSrc @15:38:40.29 delay 3 seconds @15:38:43.31 ATV.dial :ElutionWater @15:38:44.40 AS.seek 0.12ml @15:38:50.72 ATV.dial :air3 @15:38:51.34 AS.seek 0.13ml @15:38:53.66 delay 2 seconds @15:38:55.67 ATV.dial :bleach @15:38:56.19 AS.seek 0.23ml @15:39:16.68 delay 3 seconds @15:39:19.70 ATV.dial :air3 @15:39:20.24 delay 3 seconds @15:39:23.32 AS.seek 0.255ml @15:39:28.67 delay 3 seconds @15:39:31.69 ATV.dial :bleach @15:39:32.31 AS.seek 0.24ml @15:39:35.63 delay 3 seconds @15:39:36.00 Stroke completed -- waiting for pressure to equalize ... @15:39:38.64 ATV.dial :ASV @15:39:39.64 ASV.dial :SPEdelivery @15:39:40.01 AFV.select :carrierSolution @15:39:40.13 delay 2 seconds @15:39:41.78 Intake.select :close @15:39:41.94 SSV.dial :exhaust @15:39:42.15 AS.reconfigure ASconfig @15:39:42.20 AS.seek 0.5ml @15:39:43.24 Exhaust.select :open @15:39:43.44 SS.reconfigure SSexhale @15:39:43.50 SS.seek 0ml @15:39:52.70 Exhaust.select :close @15:39:52.82 SSV.dial CBV @15:39:52.85 CBV.dial SSV @15:39:53.71 Allowing 130s for 25.0ml of remaining 650.0ml @15:39:53.72 Intake.select :open @15:39:55.91 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:39:55.93 SS.reconfigure SSinhale.with maxSpeed: 29 @15:39:55.98 SS.seek 25ml @15:39:57.44 delay 1 second @15:39:58.45 AFV.select :holdingCoil @15:39:58.61 AS.reconfigure ASslow1 @15:39:58.67 AS.seek 0.17ml @15:40:56.59 Stroke completed -- waiting for pressure to equalize ... @15:41:03.02 Intake.select :close @15:41:03.19 SSV.dial :exhaust @15:41:04.01 Exhaust.select :open @15:41:04.12 SS.reconfigure SSexhale @15:41:04.17 SS.seek 0ml @15:41:05.38 SPE.hold 2:46:40,95C @15:41:05.45 delay 3:00 @15:41:13.27 Exhaust.select :close @15:41:13.39 SSV.dial CBV @15:41:13.42 CBV.dial SSV @15:41:14.28 Allowing 130s for 25.0ml of remaining 625.0ml @15:41:14.29 Intake.select :open @15:41:16.48 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:41:16.50 SS.reconfigure SSinhale.with maxSpeed: 29 @15:41:16.54 SS.seek 25ml @15:42:05.93 Vacuum too high -- waiting up to 40s @15:42:07.54 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:42:07.56 SS.reconfigure SSinhale.with maxSpeed: 26 @15:42:07.62 SS.seek 25ml @15:42:20.27 Stroke completed -- waiting for pressure to equalize ... @15:42:26.04 Intake.select :close @15:42:26.20 SSV.dial :exhaust @15:42:27.03 Exhaust.select :open @15:42:27.15 SS.reconfigure SSexhale @15:42:27.20 SS.seek 0ml @15:42:36.53 Exhaust.select :close @15:42:36.65 SSV.dial CBV @15:42:36.68 CBV.dial SSV @15:42:37.54 Allowing 130s for 25.0ml of remaining 600.0ml @15:42:37.55 Intake.select :open @15:42:39.74 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:42:39.75 SS.reconfigure SSinhale.with maxSpeed: 26 @15:42:40.38 SS.seek 25ml @15:43:47.99 Stroke completed -- waiting for pressure to equalize ... @15:43:53.77 Intake.select :close @15:43:53.93 SSV.dial :exhaust @15:43:54.78 Exhaust.select :open @15:43:54.89 SS.reconfigure SSexhale @15:43:54.95 SS.seek 0ml @15:44:04.52 Exhaust.select :close @15:44:04.64 SSV.dial CBV @15:44:04.67 CBV.dial SSV @15:44:05.48 AFV.select :carrierSolution @15:44:05.55 Allowing 130s for 25.0ml of remaining 575.0ml @15:44:05.57 Intake.select :open @15:44:05.62 delay 2 seconds @15:44:07.65 AS.reconfigure ASconfig @15:44:07.75 AS.seek 1ml @15:44:07.80 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:44:07.85 SS.reconfigure SSinhale.with maxSpeed: 26 @15:44:07.91 SS.seek 25ml @15:44:55.81 delay 1 second @15:44:56.82 AFV.select :holdingCoil @15:44:56.93 delay 1 second @15:44:57.95 ASV.dial :wasteD @15:44:58.97 AS.seek 0ml @15:45:15.38 Stroke completed -- waiting for pressure to equalize ... @15:45:21.75 Intake.select :close @15:45:21.91 SSV.dial :exhaust @15:45:22.76 Exhaust.select :open @15:45:22.87 SS.reconfigure SSexhale @15:45:22.92 SS.seek 0ml @15:45:31.95 Exhaust.select :close @15:45:32.07 SSV.dial CBV @15:45:32.10 CBV.dial SSV @15:45:32.96 Allowing 130s for 25.0ml of remaining 550.0ml @15:45:32.97 Intake.select :open @15:45:35.15 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:45:35.17 SS.reconfigure SSinhale.with maxSpeed: 26 @15:45:35.22 SS.seek 25ml @15:45:56.76 Priming Eluate @15:45:56.77 AFV.select :carrierSolution @15:45:56.88 delay 2 seconds @15:45:58.91 AS.seek 0.5ml @15:46:27.96 delay 1 second @15:46:28.97 AFV.select :holdingCoil @15:46:29.08 ATV.dial ASV @15:46:29.09 ASV.dial :SPErecovery @15:46:29.68 Swish #1 of 2 @15:46:29.76 AS.reconfigure ASslow0 @15:46:29.81 AS.seek 0.6ml @15:46:40.30 AS.seek 0.5ml @15:46:42.94 Stroke completed -- waiting for pressure to equalize ... @15:46:49.93 Intake.select :close @15:46:50.09 SSV.dial :exhaust @15:46:50.70 Swish #2 of 2 @15:46:50.79 AS.seek 0.6ml @15:46:50.93 Exhaust.select :open @15:46:51.05 SS.reconfigure SSexhale @15:46:51.11 SS.seek 0ml @15:47:00.61 Exhaust.select :close @15:47:00.73 SSV.dial CBV @15:47:00.76 CBV.dial SSV @15:47:01.25 AS.seek 0.5ml @15:47:01.60 Allowing 130s for 25.0ml of remaining 525.0ml @15:47:01.61 Intake.select :open @15:47:03.80 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:47:03.82 SS.reconfigure SSinhale.with maxSpeed: 26 @15:47:03.87 SS.seek 25ml @15:47:11.65 delay 3 seconds @15:47:14.73 AS.reconfigure ASslow1 @15:47:14.79 AS.seek 0.684ml @15:47:52.09 delay 3 seconds @15:47:55.12 Eluate primed @15:47:55.13 ATV.dial :waste2 @15:47:55.73 AS.reconfigure ASnorm @15:47:55.79 AS.seek 0ml @15:48:04.87 Vacuum too high -- waiting up to 40s @15:48:06.50 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:48:06.52 SS.reconfigure SSinhale.with maxSpeed: 23 @15:48:06.57 SS.seek 25ml @15:48:14.66 Stroke completed -- waiting for pressure to equalize ... @15:48:20.44 Intake.select :close @15:48:20.61 SSV.dial :exhaust @15:48:21.43 Exhaust.select :open @15:48:21.54 SS.reconfigure SSexhale @15:48:21.59 SS.seek 0ml @15:48:30.61 SPE.coast @15:48:30.70 ASV.dial :SPErecovery @15:48:30.72 Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1515h150ml.pcr @15:48:30.73 Building PCR Slug from ASV with MM1 mix, PP2 primer @15:48:30.75 Priming with carrier @15:48:30.76 ATV.dial ARV @15:48:30.83 Exhaust.select :close @15:48:30.95 SSV.dial CBV @15:48:31.01 CBV.dial SSV @15:48:31.70 ARV.dial :carrier @15:48:31.82 Allowing 130s for 25.0ml of remaining 500.0ml @15:48:31.83 Intake.select :open @15:48:32.05 AS.seek 0.5ml @15:48:34.03 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:48:34.06 SS.reconfigure SSinhale.with maxSpeed: 23 @15:48:34.10 SS.seek 25ml @15:48:57.58 AFV.select :carrierSolution @15:48:57.70 delay 2 seconds @15:48:59.73 AS.reconfigure ASconfig @15:48:59.78 AS.seek 1ml @15:49:28.79 delay 1 second @15:49:29.80 AFV.select :holdingCoil @15:49:29.91 delay 1 second @15:49:30.93 ATV.dial :waste @15:49:32.37 AS.reconfigure ASnorm @15:49:32.42 AS.seek :empty @15:49:50.06 Stroke completed -- waiting for pressure to equalize ... @15:49:56.46 Intake.select :close @15:49:56.62 SSV.dial :exhaust @15:49:57.45 Exhaust.select :open @15:49:57.56 SS.reconfigure SSexhale @15:49:57.61 SS.seek 0ml @15:50:06.91 Exhaust.select :close @15:50:07.03 SSV.dial CBV @15:50:07.06 CBV.dial SSV @15:50:07.92 Allowing 130s for 25.0ml of remaining 475.0ml @15:50:07.93 Intake.select :open @15:50:10.11 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:50:10.13 SS.reconfigure SSinhale.with maxSpeed: 23 @15:50:10.18 SS.seek 25ml @15:50:23.73 AFV.select :carrierSolution @15:50:23.85 delay 2 seconds @15:50:25.88 AS.reconfigure ASconfig @15:50:25.93 AS.seek 0.5ml @15:50:55.04 delay 1 second @15:50:56.05 AFV.select :holdingCoil @15:50:56.16 Building Slug @15:50:56.17 ATV.dial :ARV @15:50:57.81 delay 2 seconds @15:50:59.83 ARV.dial :air1 @15:51:00.24 delay 2 seconds @15:51:02.33 AS.reconfigure ASslow2 @15:51:02.38 AS.seek 0.506ml @15:51:08.78 delay 2 seconds @15:51:10.80 ARV.dial :MM1 @15:51:11.40 delay 2 seconds @15:51:13.43 Adding MM1 @15:51:13.50 AS.seek 0.524ml @15:51:26.33 Stroke completed -- waiting for pressure to equalize ... @15:51:31.98 delay 25 seconds @15:51:33.45 Intake.select :close @15:51:33.61 SSV.dial :exhaust @15:51:34.42 Exhaust.select :open @15:51:34.54 SS.reconfigure SSexhale @15:51:34.59 SS.seek 0ml @15:51:43.94 Exhaust.select :close @15:51:44.06 SSV.dial CBV @15:51:44.09 CBV.dial SSV @15:51:44.93 Allowing 130s for 25.0ml of remaining 450.0ml @15:51:44.94 Intake.select :open @15:51:47.13 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:51:47.15 SS.reconfigure SSinhale.with maxSpeed: 23 @15:51:47.19 SS.seek 25ml @15:51:57.01 ARV.dial :PP2 @15:51:57.40 Adding PP2 @15:51:57.41 delay 2 seconds @15:51:59.50 AS.seek 0.53ml @15:52:05.73 delay 10 seconds @15:52:15.75 ARV.dial :air2 @15:52:16.12 adding ARV air @15:52:16.64 delay 2 seconds @15:52:18.82 AS.seek 0.536ml @15:52:25.20 delay 5 seconds @15:52:30.22 ARV.dial :carrier @15:52:31.45 delay 7 seconds @15:52:38.54 AS.seek 0.594ml @15:53:02.94 Stroke completed -- waiting for pressure to equalize ... @15:53:10.63 Intake.select :close @15:53:10.79 SSV.dial :exhaust @15:53:11.62 Exhaust.select :open @15:53:11.73 SS.reconfigure SSexhale @15:53:11.78 SS.seek 0ml @15:53:21.14 Exhaust.select :close @15:53:21.26 SSV.dial CBV @15:53:21.29 CBV.dial SSV @15:53:22.15 Allowing 130s for 25.0ml of remaining 425.0ml @15:53:22.16 Intake.select :open @15:53:24.35 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:53:24.37 SS.reconfigure SSinhale.with maxSpeed: 23 @15:53:24.42 SS.seek 25ml @15:53:37.07 delay 5 seconds @15:53:42.09 ATV.dial :ASV @15:53:42.49 delay 2 seconds @15:53:44.58 AS.seek 0.6ml @15:53:50.90 delay 4 seconds @15:53:54.92 ATV.dial ARV @15:53:55.45 delay 2 seconds @15:53:57.47 ARV.dial :carrier @15:53:57.49 delay 2 seconds @15:53:59.58 AS.seek 0.65ml @15:54:40.63 Stroke completed -- waiting for pressure to equalize ... @15:54:48.34 Intake.select :close @15:54:48.50 SSV.dial :exhaust @15:54:49.31 Exhaust.select :open @15:54:49.43 SS.reconfigure SSexhale @15:54:49.49 SS.seek 0ml @15:54:50.17 delay 4 seconds @15:54:54.18 ATV.dial PCR @15:54:54.78 ASV.dial :waste1 @15:54:55.53 ARV.dial :air1 @15:54:55.92 Positioning Slug by 0.324ml @15:54:56.01 AS.seek 0.326ml @15:54:58.41 Exhaust.select :close @15:54:58.53 SSV.dial CBV @15:54:58.56 CBV.dial SSV @15:55:00.03 Allowing 130s for 25.0ml of remaining 400.0ml @15:55:00.04 Intake.select :open @15:55:02.22 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:55:02.24 SS.reconfigure SSinhale.with maxSpeed: 23 @15:55:02.29 SS.seek 25ml @15:56:03.55 Vacuum too high -- waiting up to 40s @15:56:05.44 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:56:05.46 SS.reconfigure SSinhale.with maxSpeed: 21 @15:56:05.51 SS.seek 25ml @15:56:22.31 Stroke completed -- waiting for pressure to equalize ... @15:56:29.39 Intake.select :close @15:56:29.55 SSV.dial :exhaust @15:56:30.37 Exhaust.select :open @15:56:30.48 SS.reconfigure SSexhale @15:56:30.53 SS.seek 0ml @15:56:39.61 Exhaust.select :close @15:56:39.73 SSV.dial CBV @15:56:39.76 CBV.dial SSV @15:56:40.62 Allowing 130s for 25.0ml of remaining 375.0ml @15:56:40.63 Intake.select :open @15:56:42.82 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:56:42.84 SS.reconfigure SSinhale.with maxSpeed: 21 @15:56:42.89 SS.seek 25ml @15:58:06.50 Stroke completed -- waiting for pressure to equalize ... @15:58:14.79 Intake.select :close @15:58:14.96 SSV.dial :exhaust @15:58:15.77 Exhaust.select :open @15:58:15.89 SS.reconfigure SSexhale @15:58:15.95 SS.seek 0ml @15:58:25.61 Exhaust.select :close @15:58:25.73 SSV.dial CBV @15:58:25.76 CBV.dial SSV @15:58:26.60 Allowing 130s for 25.0ml of remaining 350.0ml @15:58:26.61 Intake.select :open @15:58:28.80 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:58:28.81 SS.reconfigure SSinhale.with maxSpeed: 21 @15:58:28.86 SS.seek 25ml @15:59:52.80 Stroke completed -- waiting for pressure to equalize ... @16:00:01.17 Intake.select :close @16:00:01.34 SSV.dial :exhaust @16:00:02.17 Exhaust.select :open @16:00:02.28 SS.reconfigure SSexhale @16:00:02.33 SS.seek 0ml @16:00:11.94 Exhaust.select :close @16:00:12.06 SSV.dial CBV @16:00:12.09 CBV.dial SSV @16:00:12.95 Allowing 130s for 25.0ml of remaining 325.0ml @16:00:12.96 Intake.select :open @16:00:15.15 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:00:15.17 SS.reconfigure SSinhale.with maxSpeed: 21 @16:00:15.22 SS.seek 25ml @16:00:22.15 delay 5 seconds @16:00:27.18 ATV.dial ARV @16:00:27.92 ASV.dial :PCRlock @16:00:28.29 delay 5 seconds @16:00:33.32 Running Ss Profile60C350 thermocycling @16:00:33.33 PCR.program Profile60C350 @16:00:33.48 Profile60C350 should complete in < 1:48:05 @16:00:33.49 PCR.run 2:20:06.25 @16:01:15.49 Vacuum too high -- waiting up to 40s @16:01:17.14 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:01:17.16 SS.reconfigure SSinhale.with maxSpeed: 19 @16:01:17.21 SS.seek 25ml @16:01:43.02 Stroke completed -- waiting for pressure to equalize ... @16:01:50.74 Intake.select :close @16:01:50.90 SSV.dial :exhaust @16:01:51.74 Exhaust.select :open @16:01:51.85 SS.reconfigure SSexhale @16:01:51.90 SS.seek 0ml @16:02:01.56 Exhaust.select :close @16:02:01.68 SSV.dial CBV @16:02:01.71 CBV.dial SSV @16:02:02.57 Allowing 130s for 25.0ml of remaining 300.0ml @16:02:02.58 Intake.select :open @16:02:04.77 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:02:04.79 SS.reconfigure SSinhale.with maxSpeed: 19 @16:02:04.84 SS.seek 25ml @16:03:37.02 Stroke completed -- waiting for pressure to equalize ... @16:03:45.36 Intake.select :close @16:03:45.52 SSV.dial :exhaust @16:03:46.34 Exhaust.select :open @16:03:46.45 SS.reconfigure SSexhale @16:03:46.51 SS.seek 0ml @16:03:56.03 Exhaust.select :close @16:03:56.15 SSV.dial CBV @16:03:56.18 CBV.dial SSV @16:03:57.01 Allowing 130s for 25.0ml of remaining 275.0ml @16:03:57.02 Intake.select :open @16:03:59.21 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:03:59.23 SS.reconfigure SSinhale.with maxSpeed: 19 @16:03:59.27 SS.seek 25ml @16:05:31.44 Stroke completed -- waiting for pressure to equalize ... @16:05:41.10 Intake.select :close @16:05:41.26 SSV.dial :exhaust @16:05:42.09 Exhaust.select :open @16:05:42.75 SS.reconfigure SSexhale @16:05:42.89 SS.seek 0ml @16:05:51.86 Exhaust.select :close @16:05:51.98 SSV.dial CBV @16:05:52.01 CBV.dial SSV @16:05:52.84 Allowing 130s for 25.0ml of remaining 250.0ml @16:05:52.86 Intake.select :open @16:05:55.03 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:05:55.05 SS.reconfigure SSinhale.with maxSpeed: 19 @16:05:55.10 SS.seek 25ml @16:07:02.01 Vacuum too high -- waiting up to 40s @16:07:03.83 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:07:03.86 SS.reconfigure SSinhale.with maxSpeed: 17 @16:07:03.91 SS.seek 25ml @16:07:32.76 Stroke completed -- waiting for pressure to equalize ... @16:07:41.72 Intake.select :close @16:07:41.88 SSV.dial :exhaust @16:07:42.70 Exhaust.select :open @16:07:42.81 SS.reconfigure SSexhale @16:07:42.88 SS.seek 0ml @16:07:51.89 Exhaust.select :close @16:07:52.01 SSV.dial CBV @16:07:52.04 CBV.dial SSV @16:07:52.90 Allowing 130s for 25.0ml of remaining 225.0ml @16:07:52.91 Intake.select :open @16:07:55.09 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:07:55.11 SS.reconfigure SSinhale.with maxSpeed: 17 @16:07:55.16 SS.seek 25ml @16:09:38.28 Stroke completed -- waiting for pressure to equalize ... @16:09:48.53 Intake.select :close @16:09:48.70 SSV.dial :exhaust @16:09:49.53 Exhaust.select :open @16:09:49.64 SS.reconfigure SSexhale @16:09:49.69 SS.seek 0ml @16:09:58.91 Exhaust.select :close @16:09:59.03 SSV.dial CBV @16:09:59.08 CBV.dial SSV @16:09:59.92 Allowing 130s for 25.0ml of remaining 200.0ml @16:09:59.96 Intake.select :open @16:10:02.17 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:10:02.19 SS.reconfigure SSinhale.with maxSpeed: 17 @16:10:02.24 SS.seek 25ml @16:11:44.92 Stroke completed -- waiting for pressure to equalize ... @16:11:55.83 Intake.select :close @16:11:55.99 SSV.dial :exhaust @16:11:56.81 Exhaust.select :open @16:11:56.92 SS.reconfigure SSexhale @16:11:56.97 SS.seek 0ml @16:12:06.20 Exhaust.select :close @16:12:06.32 SSV.dial CBV @16:12:06.35 CBV.dial SSV @16:12:07.20 Allowing 130s for 25.0ml of remaining 175.0ml @16:12:07.21 Intake.select :open @16:12:09.40 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:12:09.42 SS.reconfigure SSinhale.with maxSpeed: 17 @16:12:09.46 SS.seek 25ml @16:13:52.63 Stroke completed -- waiting for pressure to equalize ... @16:14:04.79 Intake.select :close @16:14:04.95 SSV.dial :exhaust @16:14:05.77 Exhaust.select :open @16:14:05.89 SS.reconfigure SSexhale @16:14:05.95 SS.seek 0ml @16:14:15.16 Exhaust.select :close @16:14:15.28 SSV.dial CBV @16:14:15.31 CBV.dial SSV @16:14:16.15 Allowing 130s for 25.0ml of remaining 150.0ml @16:14:16.16 Intake.select :open @16:14:18.35 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:14:18.37 SS.reconfigure SSinhale.with maxSpeed: 17 @16:14:18.41 SS.seek 25ml @16:15:06.58 Vacuum too high -- waiting up to 40s @16:15:08.73 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:15:08.75 SS.reconfigure SSinhale.with maxSpeed: 15 @16:15:08.80 SS.seek 25ml @16:16:11.17 Stroke completed -- waiting for pressure to equalize ... @16:16:22.10 Intake.select :close @16:16:22.27 SSV.dial :exhaust @16:16:23.12 Exhaust.select :open @16:16:23.23 SS.reconfigure SSexhale @16:16:23.28 SS.seek 0ml @16:16:32.66 Exhaust.select :close @16:16:32.78 SSV.dial CBV @16:16:32.81 CBV.dial SSV @16:16:33.65 Allowing 130s for 25.0ml of remaining 125.0ml @16:16:33.66 Intake.select :open @16:16:35.84 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:16:35.86 SS.reconfigure SSinhale.with maxSpeed: 15 @16:16:35.91 SS.seek 25ml @16:18:32.38 Stroke completed -- waiting for pressure to equalize ... @16:18:43.68 SS.reconfigure SSconfig @16:18:43.73 SS.hold @16:18:43.87 Intake.select :close @16:18:44.10 ShallowSampler::Clogged in WCR -- progress too slow after 1400.0ml @16:18:44.11 SSV.dial :exhaust @16:18:44.95 Exhaust.select :open @16:18:45.12 SS.reconfigure SSexhale @16:18:45.22 SS.seek 0ml @16:18:54.58 Exhaust.select :close @16:18:54.70 Sampled 1400.0ml @16:18:54.72 Drying puck before fixing for 4:00 @16:18:54.73 CBV.dial :waste2b @16:18:55.55 delay 1:00 @16:19:55.57 CSV.dial :air @16:19:56.05 CS.reconfigure CSfast @16:19:56.11 CS.seek 10ml @16:20:21.29 Pumping wcr puck from tube 3 up to 55psi... @16:20:21.30 CBV.dial SSV @16:20:22.69 CBV.dial between CSV and waste2b @16:20:24.37 CSV.dial between air and CTV @16:20:24.74 CS.seek 6.887ml @16:20:33.29 CTV.dial CSV,avoiding: :air @16:20:33.32 CSV.dial CTV @16:20:34.19 CS.reconfigure CSpressurize @16:20:34.25 CS.seek 0ml @16:20:52.40 CSV.dial :air @16:20:52.91 CS.reconfigure CSfast @16:20:52.97 CS.seek 10ml @16:21:17.97 CSV.dial between air and CTV @16:21:18.33 CS.seek 2.496ml @16:21:37.60 CTV.dial CSV,avoiding: :air @16:21:37.63 CSV.dial CTV @16:21:37.96 CS.reconfigure CSpressurize @16:21:38.02 CS.seek 0ml @16:21:42.50 CBV.dial :waste2b @16:21:42.86 CS.reconfigure CSconfig @16:21:42.92 CS.seek :empty @16:21:46.51 delay 1:00 @16:22:46.54 CTV.dial :air,avoiding: :intake @16:22:47.96 Fix #1 of 2 (with rnal) for 2:00 @16:22:47.98 CSV.dial CSR @16:22:48.52 Pulling 2ml of CSR.rnal @16:22:48.53 CSR.select :rnal @16:22:48.66 CS.seek 2ml @16:22:54.79 delay 5 seconds @16:22:59.81 CSR.select :air @16:22:59.94 CS.reconfigure CSfast @16:23:00.00 CS.seek 10ml @16:23:19.98 Pumping wcr puck from tube 3 up to 55psi... @16:23:19.99 CBV.dial SSV @16:23:21.37 CBV.dial between CSV and waste2b @16:23:22.44 CSV.dial between air and CTV @16:23:23.11 CS.seek 6.781ml @16:23:31.76 CTV.dial CSV,avoiding: :air @16:23:31.79 CSV.dial CTV @16:23:33.29 CS.reconfigure CSpressurize @16:23:33.35 CS.seek 0ml @16:23:51.28 CSV.dial :air @16:23:51.79 CS.reconfigure CSfast @16:23:51.85 CS.seek 10ml @16:24:17.42 CSV.dial between air and CTV @16:24:17.77 CS.seek 2.044ml @16:24:38.04 CTV.dial CSV,avoiding: :air @16:24:38.07 CSV.dial CTV @16:24:38.42 CS.reconfigure CSpressurize @16:24:38.48 CS.seek 0ml @16:24:39.91 delay 2:00 @16:26:39.93 CBV.dial :waste2b @16:26:40.29 CS.reconfigure CSconfig @16:26:40.35 CS.seek :empty @16:26:45.73 delay 1:00 @16:27:45.76 CTV.dial :air,avoiding: :intake @16:27:47.18 Fix #2 of 2 (with rnal) for 2:00 @16:27:47.20 CSV.dial CSR @16:27:47.74 Pulling 2ml of CSR.rnal @16:27:47.75 CSR.select :rnal @16:27:47.88 CS.seek 2ml @16:27:54.01 delay 5 seconds @16:27:59.03 CSR.select :air @16:27:59.16 CS.reconfigure CSfast @16:27:59.22 CS.seek 10ml @16:28:19.23 Pumping wcr puck from tube 3 up to 55psi... @16:28:19.24 CBV.dial SSV @16:28:20.64 CBV.dial between CSV and waste2b @16:28:21.71 CSV.dial between air and CTV @16:28:22.38 CS.seek 6.781ml @16:28:31.08 CTV.dial CSV,avoiding: :air @16:28:31.11 CSV.dial CTV @16:28:32.62 CS.reconfigure CSpressurize @16:28:32.68 CS.seek 0ml @16:28:50.60 CSV.dial :air @16:28:51.11 CS.reconfigure CSfast @16:28:51.17 CS.seek 10ml @16:29:15.87 CSV.dial between air and CTV @16:29:16.23 CS.seek 2.06ml @16:29:36.14 CTV.dial CSV,avoiding: :air @16:29:36.73 CSV.dial CTV @16:29:37.12 CS.reconfigure CSpressurize @16:29:37.17 CS.seek 0ml @16:29:38.63 delay 2:00 @16:31:38.65 CBV.dial :waste2b @16:31:39.03 CS.reconfigure CSconfig @16:31:39.08 CS.seek :empty @16:31:44.42 delay 1:00 @16:32:44.46 CTV.dial :air,avoiding: :intake @16:32:45.93 Storing wcr puck in Collection Clamp to tube 2 @16:32:46.13 SC.reconfigure SCtight @16:32:46.18 SC.seek 2 @16:32:48.33 SC.reconfigure SCloose @16:32:48.56 Elbow.reconfigure TightElbow @16:32:48.61 Elbow.seek 2 @16:32:50.62 Forearm.reconfigure TightForearm @16:32:50.67 Forearm.seek 2 + 700 counts @16:32:51.68 SE.reconfigure SEpick @16:32:51.75 SE.seek beamBreak @16:32:53.44 SE.configure SEconfig @16:32:53.61 Elbow.reconfigure LimpElbow @16:32:53.67 Forearm.reconfigure LooseForearm @16:32:53.72 Forearm.seek 2 @16:32:54.10 Elbow.hold @16:32:54.17 Elbow.reconfigure TightElbow @16:32:54.29 SE.seek 15.09 Pucks below Top Plate @16:32:54.72 Forearm.force -20 @16:32:54.77 Forearm.seek 2 + 341 counts @16:32:55.11 Forearm.reconfigure TightForearm @16:32:55.17 Forearm.seek 2 + 700 counts @16:32:55.62 SE.seek 16.09 Pucks below Top Plate @16:32:56.30 Forearm.reconfigure LooseForearm @16:32:56.35 Forearm.seek :retract @16:32:57.32 Elbow.seek CC @16:32:59.00 Elbow.reconfigure LooseElbow @16:32:59.11 Forearm.seek CC @16:33:00.07 Hand.close @16:33:00.78 CC.seek :open @16:33:05.93 Forearm.seek :retract @16:33:06.94 Elbow.reconfigure TightElbow @16:33:06.99 Elbow.seek 2 @16:33:08.89 Elbow.reconfigure LimpElbow @16:33:08.95 Forearm.seek 2 @16:33:09.96 Elbow.hold @16:33:10.04 Elbow.reconfigure TightElbow @16:33:10.09 Hand.open @16:33:11.49 Forearm.force -20 @16:33:11.54 Forearm.seek 2 + 345 counts @16:33:11.88 Forearm.reconfigure TightForearm @16:33:11.94 Forearm.seek 2 + 700 counts @16:33:12.33 SE.seek :down @16:33:13.48 Forearm.reconfigure LooseForearm @16:33:13.53 Forearm.seek :retract @16:33:13.63 SE.coast @16:33:14.44 SC.coast @16:33:14.48 Starting finalPurge @16:33:14.50 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:33:14.58 Hand.open @16:33:14.91 Elbow.seek FlushPuck::Garage @16:33:16.95 Elbow.reconfigure LooseElbow @16:33:17.06 Forearm.seek :garage @16:33:18.08 Hand.close @16:33:18.78 Forearm.seek :retract @16:33:19.82 Elbow.reconfigure TightElbow @16:33:19.87 Elbow.seek CC @16:33:22.78 Elbow.reconfigure LooseElbow @16:33:22.89 Forearm.reconfigure TightForearm @16:33:22.94 Forearm.seek CC @16:33:24.22 CC.seek :closed @16:33:28.43 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.07 @16:33:28.52 Hand.open @16:33:29.22 Forearm.reconfigure LooseForearm @16:33:29.27 Forearm.seek :retract @16:33:30.30 Flushing the Collection Valve::Manifold @16:33:31.06 CSV.dial CSR @16:33:31.72 Pulling 5ml of CSR.flush @16:33:31.73 CSR.select :flush @16:33:31.86 CS.seek 5ml @16:33:45.71 delay 5 seconds @16:33:50.73 CSR.select :air @16:33:50.91 CS.reconfigure CSfast @16:33:50.96 CS.seek 7.5ml @16:33:57.95 CSV.dial :waste @16:33:59.35 CS.reconfigure CSconfig @16:33:59.40 CS.seek 0ml @16:34:19.52 Flushing lower manifold's waste1 port @16:34:19.68 CSV.dial :flush @16:34:20.17 CS.reconfigure CSfast @16:34:20.22 CS.seek 5ml @16:34:33.05 delay 5 seconds @16:34:38.06 CSV.dial :air @16:34:38.64 CS.seek 7ml @16:34:44.36 CSV.dial CTV @16:34:44.39
CTV.dial CSV @16:34:45.91 CBV.dial :waste1 @16:34:46.75 CS.seek 0ml @16:35:04.27 Flushing lower manifold's waste2b port @16:35:04.41 CSV.dial :flush @16:35:05.20 CS.seek 5ml @16:35:17.93 delay 5 seconds @16:35:22.95 CSV.dial :air @16:35:23.55 CS.seek 7ml @16:35:29.30 CSV.dial CTV @16:35:29.33
CTV.dial CSV @16:35:29.80 CBV.dial :waste2b @16:35:30.64 CS.seek 0ml @16:35:48.49 Flushing upper manifold @16:35:48.63 CSV.dial :flush @16:35:49.44 CS.seek 5ml @16:36:02.24 delay 5 seconds @16:36:07.25 CSV.dial :air @16:36:07.84 CS.seek 7ml @16:36:13.68 CSV.dial CBV @16:36:13.71
CBV.dial CSV @16:36:14.88 CTV.dial :air @16:36:16.29 CS.seek 0ml @16:36:33.68 delay 7 seconds @16:36:40.70 CS.seek 3ml @16:36:48.89 CSV.dial :waste @16:36:49.41 CS.seek 0ml @16:36:57.68 CSV.dial :flush @16:36:58.17 CS.seek 3ml @16:37:06.33 delay 5 seconds @16:37:11.34 CSV.dial :air @16:37:11.90 CS.seek 5ml @16:37:17.73 CSV.dial :air @16:37:17.76 CS.seek 0ml @16:37:31.18 delay 7 seconds @16:37:38.21 CS.seek 3ml @16:37:46.32 CSV.dial :waste @16:37:47.14 CS.seek 0ml @16:37:55.18 Disinfecting external Intake with mfbkill @16:37:55.32 CSV.dial CSR @16:37:56.77 Pulling 8ml of CSR.mfbkill @16:37:56.78 CSR.select :mfbkill @16:37:56.91 CS.seek 8ml @16:38:16.87 delay 5 seconds @16:38:21.89 CSR.select :air @16:38:22.03 CS.seek 10ml @16:38:27.99 CSV.dial CBV @16:38:28.02
CBV.dial CSV @16:38:29.12 CTV.dial :intake @16:38:29.93 Intake.select :open @16:38:30.05 CS.seek 2ml @16:38:50.76 Intake.select :close @16:38:50.88 CTV.dial :air @16:38:51.68 CS.reconfigure CSconfig @16:38:51.73 CS.seek 0ml @16:38:57.87 Flushing lower manifold's waste1 port @16:38:57.88 CSV.dial CSR @16:38:59.16 Pulling 4ml of CSR.mfbkill @16:38:59.17 CSR.select :mfbkill @16:38:59.30 CS.seek 4ml @16:39:10.74 delay 5 seconds @16:39:15.76 CSR.select :air @16:39:15.89 CS.reconfigure CSfast @16:39:15.94 CS.seek 10ml @16:39:31.21 CSV.dial CTV @16:39:31.24
CTV.dial CSV @16:39:32.75 CBV.dial :waste1 @16:39:33.27 CS.seek 0ml @16:39:58.02 Flushing lower manifold's waste2b port @16:39:58.03 CSV.dial CSR @16:39:58.58 Pulling 4ml of CSR.mfbkill @16:39:58.59 CSR.select :mfbkill @16:39:58.72 CS.reconfigure CSconfig @16:39:58.76 CS.seek 4ml @16:40:10.15 delay 5 seconds @16:40:15.16 CSR.select :air @16:40:15.28 CS.reconfigure CSfast @16:40:15.33 CS.seek 10ml @16:40:31.09 CSV.dial CTV @16:40:31.12
CTV.dial CSV @16:40:31.63 CBV.dial :waste2b @16:40:32.47 CS.seek 0ml @16:40:56.90 Killing upper manifold @16:40:56.91 CSV.dial CSR @16:40:57.46 Pulling 4ml of CSR.mfbkill @16:40:57.47 CSR.select :mfbkill @16:40:57.60 CS.reconfigure CSconfig @16:40:57.64 CS.seek 4ml @16:41:08.81 delay 5 seconds @16:41:13.82 CSR.select :air @16:41:13.95 CS.reconfigure CSfast @16:41:14.00 CS.seek 10ml @16:41:29.18 CSV.dial CBV @16:41:29.22
CBV.dial CSV @16:41:30.37 CTV.dial :air @16:41:31.80 CS.seek 0ml @16:41:57.03 delay 7 seconds @16:42:04.06 CS.seek 3ml @16:42:12.21 CSV.dial :waste @16:42:12.69 CS.seek 0ml @16:42:20.78 Flushing upper manifold @16:42:20.92 CSV.dial :flush @16:42:21.41 CS.seek 5ml @16:42:34.24 delay 5 seconds @16:42:39.26 CSV.dial :air @16:42:39.81 CS.seek 7ml @16:42:45.56 CSV.dial CBV @16:42:45.59
CBV.dial CSV @16:42:46.70 CTV.dial :air @16:42:46.72 CS.seek 0ml @16:43:04.21 delay 7 seconds @16:43:11.23 CS.seek 3ml @16:43:19.27 CSV.dial :waste @16:43:19.79 CS.seek 0ml @16:43:28.21 CSV.dial :flush @16:43:28.71 CS.seek 3ml @16:43:36.75 delay 5 seconds @16:43:41.76 CSV.dial :air @16:43:42.33 CS.seek 5ml @16:43:48.08 CSV.dial :air @16:43:48.11 CS.seek 0ml @16:44:01.00 delay 7 seconds @16:44:08.03 CS.seek 3ml @16:44:16.11 CSV.dial :waste @16:44:16.91 CS.seek 0ml @16:44:24.96 Flushing lower manifold's waste1 port @16:44:25.10 CSV.dial :flush @16:44:25.60 CS.seek 5ml @16:44:38.43 delay 5 seconds @16:44:43.44 CSV.dial :air @16:44:43.99 CS.seek 7ml @16:44:49.97 CSV.dial CTV @16:44:50.00
CTV.dial CSV @16:44:51.53 CBV.dial :waste1 @16:44:52.04 CS.seek 0ml @16:45:09.53 Flushing lower manifold's waste2b port @16:45:09.67 CSV.dial :flush @16:45:10.58 CS.seek 5ml @16:45:23.31 delay 5 seconds @16:45:28.32 CSV.dial :air @16:45:28.88 CS.seek 7ml @16:45:34.59 CSV.dial CTV @16:45:34.62
CTV.dial CSV @16:45:35.09 CBV.dial :waste2b @16:45:35.93 CS.seek 0ml @16:45:53.22 Disinfecting external Intake with kill @16:45:53.35 CSV.dial CSR @16:45:54.51 Pulling 8ml of CSR.kill @16:45:54.52 CSR.select :kill @16:45:54.65 CS.seek 8ml @16:46:14.44 delay 5 seconds @16:46:19.46 CSR.select :air @16:46:19.60 CS.seek 10ml @16:46:25.31 CSV.dial CBV @16:46:25.34
CBV.dial CSV @16:46:26.48 CTV.dial :intake @16:46:27.26 Intake.select :open @16:46:27.38 CS.seek 2ml @16:46:47.97 Intake.select :close @16:46:48.08 CTV.dial :air @16:46:48.89 CS.reconfigure CSconfig @16:46:48.94 CS.seek 0ml @16:46:55.02 CSV.dial CSR @16:46:56.20 Pulling 5ml of CSR.flush @16:46:56.21 CSR.select :flush @16:46:56.34 CS.seek 5ml @16:47:10.15 delay 5 seconds @16:47:15.17 CSR.select :air @16:47:15.31 CS.seek 10ml @16:47:28.99 CSV.dial CBV @16:47:30.09 CS.seek 0ml @16:47:56.78 Garaging FlushPuck in Collection Clamp @16:47:56.86 Elbow.reconfigure TightElbow @16:47:56.91 Elbow.seek CC @16:47:57.18 Elbow.reconfigure LooseElbow @16:47:57.29 Forearm.seek CC @16:47:58.27 Hand.close @16:47:58.92 CC.seek :open @16:48:03.29 Forearm.seek :retract @16:48:04.28 Elbow.reconfigure TightElbow @16:48:04.33 Elbow.seek :garage @16:48:07.45 Elbow.reconfigure LooseElbow @16:48:07.55 Forearm.reconfigure TightForearm @16:48:07.60 Forearm.seek :garage @16:48:08.79 Hand.open @16:48:09.49 Forearm.reconfigure LooseForearm @16:48:09.54 Forearm.seek :retract @16:48:10.59 Starting initialPurge @16:48:10.60 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:48:10.68 Hand.open @16:48:10.99 Elbow.reconfigure TightElbow @16:48:11.03 Elbow.seek FlushPuck::Garage @16:48:11.31 Elbow.reconfigure LooseElbow @16:48:11.42 Forearm.seek :garage @16:48:12.40 Hand.close @16:48:13.17 Forearm.seek :retract @16:48:14.17 Elbow.reconfigure TightElbow @16:48:14.22 Elbow.seek CC @16:48:17.07 Elbow.reconfigure LooseElbow @16:48:17.17 Forearm.reconfigure TightForearm @16:48:17.22 Forearm.seek CC @16:48:19.12 CC.seek :closed @16:48:23.37 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.19 @16:48:23.45 Hand.open @16:48:24.11 Forearm.reconfigure LooseForearm @16:48:24.16 Forearm.seek :retract @16:48:25.12 Flushing the Collection Valve::Manifold @16:48:25.26 CSV.dial CSR @16:48:26.42 Pulling 5ml of CSR.flush @16:48:26.43 CSR.select :flush @16:48:26.56 CS.seek 5ml @16:48:40.31 delay 5 seconds @16:48:45.33 CSR.select :air @16:48:45.50 CS.reconfigure CSfast @16:48:45.55 CS.seek 7.5ml @16:48:52.43 CSV.dial :waste @16:48:53.84 CS.reconfigure CSconfig @16:48:53.89 CS.seek 0ml @16:49:13.97 Purging external Intake @16:49:14.10 CSV.dial :flush @16:49:14.59 CS.reconfigure CSfast @16:49:14.64 CS.seek 10ml @16:49:39.09 delay 5 seconds @16:49:44.11 CSV.dial CBV @16:49:44.15
CBV.dial CSV @16:49:44.97 CTV.dial :intake @16:49:45.77 Intake.select :open @16:49:45.90 CS.seek 0ml @16:50:11.46 Intake.select :close @16:50:11.58 CTV.dial :air @16:50:12.39 Flushing upper manifold @16:50:12.52 CSV.dial :flush @16:50:13.32 CS.seek 5ml @16:50:26.06 delay 5 seconds @16:50:31.07 CSV.dial :air @16:50:31.63 CS.seek 7.001ml @16:50:37.38 CSV.dial CBV @16:50:37.40
CBV.dial CSV @16:50:38.52 CTV.dial :air @16:50:38.54 CS.seek 0ml @16:50:56.03 delay 7 seconds @16:51:03.05 CS.seek 3ml @16:51:11.05 CSV.dial :waste @16:51:11.55 CS.seek 0ml @16:51:19.87 CSV.dial :flush @16:51:20.33 CS.seek 3ml @16:51:28.56 delay 5 seconds @16:51:33.57 CSV.dial :air @16:51:34.13 CS.seek 4.999ml @16:51:39.80 CSV.dial :air @16:51:39.83 CS.seek 0ml @16:51:52.53 delay 7 seconds @16:51:59.55 CS.seek 3ml @16:52:07.81 CSV.dial :waste @16:52:08.61 CS.seek 0ml @16:52:16.82 Garaging FlushPuck in Collection Clamp @16:52:17.52 Elbow.reconfigure TightElbow @16:52:17.57 Elbow.seek CC @16:52:17.80 Elbow.reconfigure LooseElbow @16:52:17.91 Forearm.seek CC @16:52:18.88 Hand.close @16:52:19.67 CC.seek :open @16:52:24.07 Forearm.seek :retract @16:52:25.08 Elbow.reconfigure TightElbow @16:52:25.13 Elbow.seek :garage @16:52:28.09 Elbow.reconfigure LooseElbow @16:52:28.20 Forearm.reconfigure TightForearm @16:52:28.25 Forearm.seek :garage @16:52:29.38 Hand.open @16:52:30.12 Forearm.reconfigure LooseForearm @16:52:30.17 Forearm.seek :retract @16:52:31.15 Completed [mbaPhase] begun 14:01:18 @16:52:31.17 Preparing to sleep until Wed Oct 17 14:01:18 +0200 2018 @16:52:31.22 deferred until MFBPCR finish @16:52:31.24
Waiting for MFBPCR til 14:01CEST17-Oct-18 @16:52:31.26 Continuing at 14:01CEST17-Oct-18 @16:52:31.33 GoodNews.email "Then, in tube 3: mbaPhase\n/home/esp/esp2/mission/18tundeployv3.rb:123\n{1 => 22, 2 => 17, 3 => 5}\nCan@16:52:31, 14.4C, 70% humidity, 14.3psia, 12.886V, 1.017A, 1.017A avg, 13.10W",Subject: "Waiting until 14:01CEST17-Oct-18" @17:23:16.21 PCR.off @17:23:18.35 PCR for Ss completed -- closed /var/log/esp/Ss18oct1515h150ml.pcr @17:23:18.42 Cleaning PCR @17:23:18.43 AFV.select :carrierSolution @17:23:18.55 delay 2 seconds @17:23:20.58 AS.reconfigure ASconfig @17:23:20.63 AS.seek 0.5ml @17:23:30.93 delay 1 second @17:23:31.94 AFV.select :holdingCoil @17:23:32.05 ASV.dial :waste1 @17:23:32.44 ATV.dial :air3 @17:23:33.65 AS.reconfigure ASslow1 @17:23:33.70 AS.seek 0.51ml @17:23:35.99 delay 2 seconds @17:23:38.00 ATV.dial :bleach @17:23:38.62 AS.seek 0.81ml @17:24:39.22 delay 3 seconds @17:24:42.24 ATV.dial :air3 @17:24:42.77 delay 3 seconds @17:24:45.86 AS.seek 0.835ml @17:24:51.22 delay 3 seconds @17:24:54.24 ATV.dial :bleach @17:24:54.84 AS.seek 0.82ml @17:24:58.15 delay 3 seconds @17:25:01.17 ARV.dial :waste @17:25:01.77 delay 3 seconds @17:25:04.79 ATV.dial :ARV @17:25:05.63 AS.reconfigure ASconfig @17:25:05.69 AS.seek 0.47ml @17:25:26.09 AFV.select :carrierSolution @17:25:26.21 delay 2 seconds @17:25:28.24 AS.seek 1ml @17:25:58.99 delay 1 second @17:26:00.00 AFV.select :holdingCoil @17:26:00.11 delay 1 second @17:26:01.14 AS.reconfigure ASfast @17:26:01.19 AS.seek 0ml @17:26:28.54 AFV.select :carrierSolution @17:26:28.66 delay 2 seconds @17:26:30.69 AS.reconfigure ASconfig @17:26:30.74 AS.seek 1ml @17:27:28.57 delay 1 second @17:27:29.58 AFV.select :holdingCoil @17:27:29.69 delay 1 second @17:27:30.72 AS.reconfigure ASfast @17:27:30.77 AS.seek 0ml @17:27:58.11 AFV.select :carrierSolution @17:27:58.23 delay 2 seconds @17:28:00.26 AS.reconfigure ASconfig @17:28:00.31 AS.seek 1ml @17:28:58.09 delay 1 second @17:28:59.10 AFV.select :holdingCoil @17:28:59.21 delay 1 second @17:29:00.24 AS.reconfigure ASfast @17:29:00.29 AS.seek 0ml @17:29:27.62 ARV.dial between carrier and air1 @17:29:28.12 ATV.dial :air3 @17:29:29.33 AS.reconfigure ASslow1 @17:29:29.38 AS.seek 0.01ml @17:29:31.71 delay 2 seconds @17:29:33.72 ATV.dial :bleach @17:29:34.33 AS.seek 0.81ml @17:32:15.46 delay 3 seconds @17:32:18.48 ATV.dial :air3 @17:32:19.01 delay 3 seconds @17:32:22.10 AS.seek 0.835ml @17:32:27.47 delay 3 seconds @17:32:30.49 ATV.dial :bleach @17:32:31.09 AS.seek 0.82ml @17:32:34.37 delay 3 seconds @17:32:37.39 ATV.dial PCR @17:32:37.77 AS.reconfigure ASconfig @17:32:37.82 AS.seek :empty @17:33:25.30 PCR.program :bleachProfile @17:33:25.35 PCR program should complete in < 2:45 @17:33:25.37 PCR.run 10:00 @17:36:33.83 PCR.off @17:36:33.86 AFV.select :carrierSolution @17:36:33.98 delay 2 seconds @17:36:36.01 AS.seek 1ml @17:37:33.85 delay 1 second @17:37:34.86 AFV.select :holdingCoil @17:37:34.97 delay 1 second @17:37:36.00 AS.reconfigure ASfast @17:37:36.05 AS.seek 0ml @17:38:03.51 delay 3 seconds @17:38:06.53 AFV.select :carrierSolution @17:38:06.64 delay 2 seconds @17:38:08.67 AS.reconfigure ASconfig @17:38:08.72 AS.seek 1ml @17:39:06.53 delay 1 second @17:39:07.54 AFV.select :holdingCoil @17:39:07.65 delay 1 second @17:39:08.68 AS.reconfigure ASfast @17:39:08.73 AS.seek 0ml @17:39:36.07 delay 3 seconds @17:39:39.09 AFV.select :carrierSolution @17:39:39.20 delay 2 seconds @17:39:41.23 AS.reconfigure ASconfig @17:39:41.29 AS.seek 1ml @17:40:39.09 delay 1 second @17:40:40.10 AFV.select :holdingCoil @17:40:40.21 delay 1 second @17:40:41.24 AS.reconfigure ASfast @17:40:41.29 AS.seek 0ml @17:41:08.65 delay 3 seconds @17:41:11.67 AFV.select :carrierSolution @17:41:11.78 delay 2 seconds @17:41:13.81 AS.reconfigure ASconfig @17:41:13.89 AS.seek 1ml @17:42:11.68 delay 1 second @17:42:12.69 AFV.select :holdingCoil @17:42:12.80 delay 1 second @17:42:13.83 AS.reconfigure ASfast @17:42:13.88 AS.seek 0ml @17:42:41.23 delay 3 seconds @17:42:44.25 AFV.select :carrierSolution @17:42:44.36 delay 2 seconds @17:42:46.39 AS.reconfigure ASconfig @17:42:46.45 AS.seek 1ml @17:43:44.23 delay 1 second @17:43:45.24 AFV.select :holdingCoil @17:43:45.35 delay 1 second @17:43:46.38 AS.reconfigure ASnorm @17:43:46.43 AS.seek 0ml @17:44:37.14 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1517h150ml.pcr @17:44:37.16 Building PCR Slug from ASV with MM2 mix, PP4 primer @17:44:37.17 Priming with carrier @17:44:37.18 ATV.dial ARV @17:44:37.87 ARV.dial :carrier @17:44:38.21 AS.seek 0.5ml @17:45:03.80 AFV.select :carrierSolution @17:45:03.92 delay 2 seconds @17:45:05.95 AS.reconfigure ASconfig @17:45:06.00 AS.seek 1ml @17:45:35.01 delay 1 second @17:45:36.02 AFV.select :holdingCoil @17:45:36.13 delay 1 second @17:45:37.15 ATV.dial :waste @17:45:38.70 AS.reconfigure ASnorm @17:45:38.75 AS.seek :empty @17:46:29.52 AFV.select :carrierSolution @17:46:29.64 delay 2 seconds @17:46:31.67 AS.reconfigure ASconfig @17:46:31.72 AS.seek 0.5ml @17:47:00.79 delay 1 second @17:47:01.80 AFV.select :holdingCoil @17:47:01.91 Building Slug @17:47:01.92 ATV.dial :ARV @17:47:03.43 delay 2 seconds @17:47:05.45 ARV.dial :air1 @17:47:05.85 delay 2 seconds @17:47:07.93 AS.reconfigure ASslow2 @17:47:07.99 AS.seek 0.506ml @17:47:14.22 delay 2 seconds @17:47:16.23 ARV.dial :MM2 @17:47:17.62 delay 2 seconds @17:47:19.65 Adding MM2 @17:47:19.73 AS.seek 0.524ml @17:47:38.00 delay 25 seconds @17:48:03.02 ARV.dial :PP4 @17:48:03.42 Adding PP4 @17:48:03.43 delay 2 seconds @17:48:05.52 AS.seek 0.53ml @17:48:11.73 delay 10 seconds @17:48:21.75 ARV.dial :air3 @17:48:22.12 adding ARV air @17:48:22.13 delay 2 seconds @17:48:24.22 AS.seek 0.536ml @17:48:30.53 delay 5 seconds @17:48:35.55 ARV.dial :carrier @17:48:36.75 delay 7 seconds @17:48:43.84 AS.seek 0.594ml @17:49:42.62 delay 5 seconds @17:49:47.64 ATV.dial :ASV @17:49:48.64 delay 2 seconds @17:49:50.73 AS.seek 0.6ml @17:49:57.08 delay 4 seconds @17:50:01.10 ATV.dial ARV @17:50:01.73 delay 2 seconds @17:50:03.75 ARV.dial :carrier @17:50:03.77 delay 2 seconds @17:50:05.87 AS.seek 0.65ml @17:50:56.61 delay 4 seconds @17:51:00.63 ATV.dial PCR @17:51:01.23 ASV.dial :waste1 @17:51:01.24 ARV.dial :air1 @17:51:01.62 Positioning Slug by 0.324ml @17:51:01.71 AS.seek 0.326ml @17:56:27.70 delay 5 seconds @17:56:32.72 ATV.dial ARV @17:56:33.45 ASV.dial :PCRlock @17:56:33.92 delay 5 seconds @17:56:38.95 Running Tt1 Profile60C300 thermocycling @17:56:38.96 PCR.program Profile60C300 @17:56:39.10 Profile60C300 should complete in < 1:45:21 @17:56:39.11 PCR.run 2:16:41.25 @19:18:35.20 PCR.off @19:18:36.78 PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1517h150ml.pcr @19:18:36.85 Cleaning PCR @19:18:36.86 AFV.select :carrierSolution @19:18:36.97 delay 2 seconds @19:18:39.00 AS.reconfigure ASconfig @19:18:39.05 AS.seek 0.5ml @19:18:49.36 delay 1 second @19:18:50.37 AFV.select :holdingCoil @19:18:50.48 ASV.dial :waste1 @19:18:50.84 ATV.dial :air3 @19:18:52.08 AS.reconfigure ASslow1 @19:18:52.13 AS.seek 0.51ml @19:18:54.42 delay 2 seconds @19:18:56.44 ATV.dial :bleach @19:18:57.04 AS.seek 0.81ml @19:19:57.62 delay 3 seconds @19:20:00.64 ATV.dial :air3 @19:20:01.17 delay 3 seconds @19:20:04.26 AS.seek 0.835ml @19:20:09.54 delay 3 seconds @19:20:12.56 ATV.dial :bleach @19:20:13.16 AS.seek 0.82ml @19:20:16.46 delay 3 seconds @19:20:19.48 ARV.dial :waste @19:20:20.06 delay 3 seconds @19:20:23.08 ATV.dial :ARV @19:20:24.03 AS.reconfigure ASconfig @19:20:24.09 AS.seek 0.47ml @19:20:44.47 AFV.select :carrierSolution @19:20:44.59 delay 2 seconds @19:20:46.62 AS.seek 1ml @19:21:17.37 delay 1 second @19:21:18.38 AFV.select :holdingCoil @19:21:18.49 delay 1 second @19:21:19.52 AS.reconfigure ASfast @19:21:19.57 AS.seek 0ml @19:21:46.91 AFV.select :carrierSolution @19:21:47.03 delay 2 seconds @19:21:49.06 AS.reconfigure ASconfig @19:21:49.11 AS.seek 1ml @19:22:46.90 delay 1 second @19:22:47.91 AFV.select :holdingCoil @19:22:48.02 delay 1 second @19:22:49.05 AS.reconfigure ASfast @19:22:49.10 AS.seek 0ml @19:23:16.41 AFV.select :carrierSolution @19:23:16.53 delay 2 seconds @19:23:18.56 AS.reconfigure ASconfig @19:23:18.61 AS.seek 1ml @19:24:16.41 delay 1 second @19:24:17.42 AFV.select :holdingCoil @19:24:17.53 delay 1 second @19:24:18.56 AS.reconfigure ASfast @19:24:18.61 AS.seek 0ml @19:24:45.96 ARV.dial between carrier and air1 @19:24:46.45 ATV.dial :air3 @19:24:47.65 AS.reconfigure ASslow1 @19:24:47.71 AS.seek 0.01ml @19:24:50.09 delay 2 seconds @19:24:52.10 ATV.dial :bleach @19:24:52.71 AS.seek 0.81ml @19:27:33.89 delay 3 seconds @19:27:36.91 ATV.dial :air3 @19:27:37.44 delay 3 seconds @19:27:40.52 AS.seek 0.835ml @19:27:45.82 delay 3 seconds @19:27:48.84 ATV.dial :bleach @19:27:49.46 AS.seek 0.82ml @19:27:53.34 delay 3 seconds @19:27:56.37 ATV.dial PCR @19:27:56.75 AS.reconfigure ASconfig @19:27:56.80 AS.seek :empty @19:28:44.26 PCR.program :bleachProfile @19:28:44.33 PCR program should complete in < 2:45 @19:28:44.34 PCR.run 10:00 @19:31:55.25 PCR.off @19:31:55.28 AFV.select :carrierSolution @19:31:55.40 delay 2 seconds @19:31:57.43 AS.seek 1ml @19:32:55.27 delay 1 second @19:32:56.28 AFV.select :holdingCoil @19:32:56.39 delay 1 second @19:32:57.42 AS.reconfigure ASfast @19:32:57.47 AS.seek 0ml @19:33:24.83 delay 3 seconds @19:33:27.85 AFV.select :carrierSolution @19:33:27.96 delay 2 seconds @19:33:29.99 AS.reconfigure ASconfig @19:33:30.04 AS.seek 1ml @19:34:27.78 delay 1 second @19:34:28.79 AFV.select :holdingCoil @19:34:28.90 delay 1 second @19:34:29.93 AS.reconfigure ASfast @19:34:29.98 AS.seek 0ml @19:34:57.30 delay 3 seconds @19:35:00.32 AFV.select :carrierSolution @19:35:00.43 delay 2 seconds @19:35:02.46 AS.reconfigure ASconfig @19:35:02.52 AS.seek 1ml @19:36:00.32 delay 1 second @19:36:01.33 AFV.select :holdingCoil @19:36:01.44 delay 1 second @19:36:02.47 AS.reconfigure ASfast @19:36:02.52 AS.seek 0ml @19:36:29.85 delay 3 seconds @19:36:32.87 AFV.select :carrierSolution @19:36:32.98 delay 2 seconds @19:36:35.01 AS.reconfigure ASconfig @19:36:35.06 AS.seek 1ml @19:37:32.83 delay 1 second @19:37:33.84 AFV.select :holdingCoil @19:37:33.95 delay 1 second @19:37:34.98 AS.reconfigure ASfast @19:37:35.03 AS.seek 0ml @19:38:02.39 delay 3 seconds @19:38:05.41 AFV.select :carrierSolution @19:38:05.52 delay 2 seconds @19:38:07.55 AS.reconfigure ASconfig @19:38:07.61 AS.seek 1ml @19:39:05.41 delay 1 second @19:39:06.42 AFV.select :holdingCoil @19:39:06.53 delay 1 second @19:39:07.56 AS.reconfigure ASnorm @19:39:07.61 AS.seek 0ml @19:39:58.31 Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1519h150ml.pcr @19:39:58.33 Building PCR Slug from ASV with MM1 mix, PP1 primer @19:39:58.35 Priming with carrier ATV.dial ARV @19:39:59.04 ARV.dial :carrier @19:39:59.39 AS.seek 0.5ml @19:40:24.95 AFV.select :carrierSolution @19:40:25.07 delay 2 seconds @19:40:27.10 AS.reconfigure ASconfig @19:40:27.15 AS.seek 1ml @19:40:56.18 delay 1 second @19:40:57.19 AFV.select :holdingCoil @19:40:57.30 delay 1 second @19:40:58.32 ATV.dial :waste @19:40:59.86 AS.reconfigure ASnorm @19:40:59.91 AS.seek :empty @19:41:50.65 AFV.select :carrierSolution @19:41:50.77 delay 2 seconds @19:41:52.80 AS.reconfigure ASconfig @19:41:52.85 AS.seek 0.5ml @19:42:21.96 delay 1 second @19:42:22.97 AFV.select :holdingCoil @19:42:23.08 Building Slug @19:42:23.09 ATV.dial :ARV @19:42:24.69 delay 2 seconds @19:42:26.71 ARV.dial :air1 @19:42:27.09 delay 2 seconds @19:42:29.17 AS.reconfigure ASslow2 @19:42:29.23 AS.seek 0.506ml @19:42:35.52 delay 2 seconds @19:42:37.53 ARV.dial :MM1 @19:42:38.13 delay 2 seconds @19:42:40.17 Adding MM1 @19:42:40.24 AS.seek 0.524ml @19:42:58.55 delay 25 seconds @19:43:23.57 ARV.dial :PP1 @19:43:23.96 Adding PP1 @19:43:23.97 delay 2 seconds @19:43:26.06 AS.seek 0.53ml @19:43:32.52 delay 10 seconds @19:43:42.54 ARV.dial :air1 @19:43:42.91 adding ARV air @19:43:42.92 delay 2 seconds @19:43:45.62 AS.seek 0.536ml @19:43:51.99 delay 5 seconds @19:43:57.01 ARV.dial :carrier @19:43:57.41 delay 7 seconds @19:44:04.50 AS.seek 0.594ml @19:45:03.02 delay 5 seconds @19:45:08.04 ATV.dial :ASV @19:45:08.44 delay 2 seconds @19:45:10.53 AS.seek 0.6ml @19:45:16.71 delay 4 seconds @19:45:20.73 ATV.dial ARV @19:45:21.26 delay 2 seconds @19:45:23.28 ARV.dial :carrier @19:45:23.30 delay 2 seconds @19:45:25.39 AS.seek 0.65ml @19:46:15.91 delay 4 seconds @19:46:19.93 ATV.dial PCR @19:46:20.51 ASV.dial :waste1 @19:46:20.52 ARV.dial :air1 @19:46:20.92 Positioning Slug by 0.324ml @19:46:21.01 AS.seek 0.326ml @19:51:47.26 delay 5 seconds @19:51:52.29 ATV.dial ARV @19:51:53.01 ASV.dial :PCRlock @19:51:53.38 delay 5 seconds @19:51:58.41 Running IPc ProfileIPc thermocycling @19:51:58.42 PCR.program ProfileIPc @19:51:58.57 ProfileIPc should complete in < 1:50:49 @19:51:58.58 PCR.run 2:23:31.25 @21:16:22.21 PCR.off @21:16:24.82 PCR for IPc completed -- closed /var/log/esp/IPc18oct1519h150ml.pcr @21:16:24.84 Full MFB Decontamination @21:16:24.85 Decon extraction column and decon AIV @21:16:24.87 SPE.hold 2:46:40,55C @21:16:24.92 ASV.dial :SPEdelivery @21:16:25.62 delay 20 seconds @21:16:45.64 AFV.select :carrierSolution @21:16:45.75 delay 2 seconds @21:16:47.78 AS.reconfigure ASconfig @21:16:47.83 AS.seek 0.5ml @21:16:58.15 delay 1 second @21:16:59.16 AFV.select :holdingCoil @21:16:59.27 ATV.dial :air1 @21:17:01.06 AS.reconfigure ASslow1 @21:17:01.11 AS.seek 0.52ml @21:17:05.39 ATV.dial :ASV @21:17:07.04 ASV.dial :SPErecovery @21:17:07.70 AS.seek 0.62ml @21:17:28.07 ATV.dial :waste @21:17:30.04 AS.reconfigure ASnorm @21:17:30.09 AS.seek :empty @21:18:01.72 AFV.select :holdingCoil @21:18:01.74 ATV.dial :waste @21:18:02.33 AS.reconfigure ASconfig @21:18:02.38 AS.seek 0ml @21:18:02.70 AFV.select :carrierSolution @21:18:02.82 delay 2 seconds @21:18:04.85 AS.seek 0.5ml @21:18:33.88 delay 1 second @21:18:34.89 AFV.select :holdingCoil @21:18:35.00 ATV.dial :air3 @21:18:35.70 AS.reconfigure ASslow1 @21:18:35.75 AS.seek 0.52ml @21:18:40.07 ATV.dial :air3 @21:18:40.16 AS.seek 0.53ml @21:18:42.46 delay 2 seconds @21:18:44.48 ATV.dial :bleach @21:18:44.95 AS.seek 0.71ml @21:19:21.41 delay 3 seconds @21:19:24.43 ATV.dial :air3 @21:19:24.99 delay 3 seconds @21:19:28.08 AS.seek 0.735ml @21:19:33.39 delay 3 seconds @21:19:36.41 ATV.dial :bleach @21:19:37.05 AS.seek 0.72ml @21:19:40.35 delay 3 seconds @21:19:43.38 ATV.dial ASV @21:19:44.38 ASV.dial :SPErecovery @21:19:44.46 AS.seek 0.365ml @21:20:56.11 ATV.dial :waste @21:20:57.77 AS.reconfigure ASnorm @21:20:57.82 AS.seek :empty @21:21:16.62 ATV.dial :air3 @21:21:17.28 AS.reconfigure ASslow1 @21:21:17.33 AS.seek 0.009ml @21:21:19.60 delay 2 seconds @21:21:21.61 ATV.dial :bleach @21:21:22.08 AS.seek 0.579ml @21:23:16.99 delay 3 seconds @21:23:20.01 ATV.dial :air3 @21:23:20.56 delay 3 seconds @21:23:23.65 AS.seek 0.604ml @21:23:28.87 delay 3 seconds @21:23:31.89 ATV.dial :bleach @21:23:32.50 AS.seek 0.589ml @21:23:35.74 delay 3 seconds @21:23:38.75 ATV.dial :ASV @21:23:39.84 ASV.dial :wasteR @21:23:40.32 AS.seek 0ml @21:25:39.07 delay 1:00 @21:26:39.10 ATV.dial ASV @21:26:39.11 AFV.select :carrierSolution @21:26:39.22 delay 2 seconds @21:26:41.25 AS.reconfigure ASconfig @21:26:41.30 AS.seek 1ml @21:27:39.06 delay 1 second @21:27:40.07 AFV.select :holdingCoil @21:27:40.18 delay 1 second @21:27:41.20 ASV.dial :wasteR @21:27:41.22 AS.reconfigure ASslow1 @21:27:41.27 AS.seek 0ml @21:31:02.68 delay 10 seconds @21:31:12.70 ATV.dial :air3 @21:31:14.13 AS.seek 0.01ml @21:31:16.51 delay 2 seconds @21:31:18.52 ATV.dial :bleach @21:31:19.13 AS.seek 0.58ml @21:33:14.03 delay 3 seconds @21:33:17.05 ATV.dial :air3 @21:33:17.59 delay 3 seconds @21:33:20.68 AS.seek 0.605ml @21:33:26.06 delay 3 seconds @21:33:29.08 ATV.dial :bleach @21:33:29.68 AS.seek 0.59ml @21:33:32.92 delay 3 seconds @21:33:35.95 ATV.dial ASV @21:33:36.91 ASV.dial :SPEdelivery @21:33:37.38 AS.seek 0ml @21:35:36.28 delay 1:00 @21:36:36.30 AFV.select :carrierSolution @21:36:36.41 delay 2 seconds @21:36:38.44 AS.reconfigure ASconfig @21:36:38.49 AS.seek 1ml @21:37:36.23 delay 1 second @21:37:37.24 AFV.select :holdingCoil @21:37:37.35 delay 1 second @21:37:38.37 ATV.dial ASV @21:37:38.39 ASV.dial :SPEdelivery @21:37:38.41 AS.reconfigure ASslow1 @21:37:38.45 AS.seek 0ml @21:40:59.81 delay 10 seconds @21:41:10.37 ATV.dial :air2 @21:41:11.25 AS.reconfigure ASslow0 @21:41:11.31 AS.seek 0.8ml @21:42:32.08 delay 3 seconds @21:42:35.10 ATV.dial :ASV @21:42:36.05 ASV.dial :SPEdelivery @21:42:36.07 AS.reconfigure ASslow1 @21:42:36.12 AS.seek 0.03ml @21:45:11.28 delay 20 seconds @21:45:31.32 ATV.dial :waste,via: :air3 @21:45:32.94 AS.reconfigure ASconfig @21:45:32.99 AS.seek :empty @21:45:35.07 SPE.coast @21:45:35.16 Removing Bleach from Mixing Coil @21:45:35.17 AFV.select :carrierSolution @21:45:35.28 delay 2 seconds @21:45:37.31 AS.seek 0.5ml @21:46:06.44 delay 1 second @21:46:07.45 AFV.select :holdingCoil @21:46:07.56 ATV.dial :ASV @21:46:09.14 ASV.dial :SPErecovery @21:46:09.77 AS.reconfigure ASslow1 @21:46:09.82 AS.seek 0.73ml @21:46:56.33 ASV.dial :wasteD @21:46:56.97 AS.reconfigure ASnorm @21:46:57.03 AS.seek 0ml @21:47:34.13 AFV.select :carrierSolution @21:47:34.25 delay 2 seconds @21:47:36.28 AS.reconfigure ASconfig @21:47:36.33 AS.seek 0.5ml @21:48:05.39 delay 1 second @21:48:06.40 AFV.select :holdingCoil @21:48:06.51 ATV.dial ASV @21:48:06.52 ASV.dial :SPErecovery @21:48:07.15 AS.reconfigure ASslow0 @21:48:07.20 AS.seek 0.3ml @21:48:27.60 ATV.dial :air2 @21:48:28.59 AS.reconfigure ASslow1 @21:48:28.65 AS.seek 0.32ml @21:48:32.96 ATV.dial ASV @21:48:33.95 AS.reconfigure ASslow0 @21:48:34.00 AS.seek 0.57ml @21:48:59.44 ASV.dial :wasteD @21:49:00.00 AS.reconfigure ASnorm @21:49:00.05 AS.seek 0ml @21:49:29.13 AFV.select :carrierSolution @21:49:29.25 delay 2 seconds @21:49:31.28 AS.reconfigure ASconfig @21:49:31.34 AS.seek 0.5ml @21:50:00.38 delay 1 second @21:50:01.39 AFV.select :holdingCoil @21:50:01.50 ATV.dial ASV @21:50:01.51 ASV.dial :SPErecovery @21:50:02.13 AS.reconfigure ASslow0 @21:50:02.18 AS.seek 0.3ml @21:50:22.62 ATV.dial :air2 @21:50:23.62 AS.reconfigure ASslow1 @21:50:23.67 AS.seek 0.32ml @21:50:27.98 ATV.dial ASV @21:50:28.98 AS.reconfigure ASslow0 @21:50:29.03 AS.seek 0.57ml @21:50:54.51 ASV.dial :wasteD @21:50:55.08 AS.reconfigure ASnorm @21:50:55.13 AS.seek 0ml @21:51:24.19 ATV.dial :air2 @21:51:25.11 AS.reconfigure ASslow0 @21:51:25.17 AS.seek 0.3ml @21:51:55.69 ATV.dial :ASV @21:51:56.62 ASV.dial :wasteD @21:51:56.65 AS.reconfigure ASconfig @21:51:56.70 AS.seek 0.05ml @21:52:11.37 ATV.dial :waste @21:52:13.11 AS.seek 0ml @21:52:16.27 ASV.dial :SPEdelivery @21:52:17.20 deconLysateINT @21:52:17.21 ATV.dial :air3 @21:52:17.87 AS.reconfigure ASslow1 @21:52:17.92 AS.seek 0.01ml @21:52:20.28 delay 2 seconds @21:52:22.29 ATV.dial :bleach @21:52:22.76 AS.seek 0.96ml @21:55:34.05 delay 3 seconds @21:55:37.07 ATV.dial :air3 @21:55:37.60 delay 3 seconds @21:55:41.31 AS.seek 0.985ml @21:55:46.64 delay 3 seconds @21:55:49.66 ATV.dial :bleach @21:55:50.26 AS.seek 0.97ml @21:55:53.53 delay 3 seconds @21:55:56.56 ATV.dial AIV @21:55:57.72 AIV.dial :coilToMFB @21:55:57.73 AWV.select :waste @21:55:57.74 AFV.select :carrierSolution @21:55:57.86 delay 2 seconds @21:55:59.89 AS.reconfigure ASconfig @21:55:59.94 AS.seek 1ml @21:56:01.97 delay 1 second @21:56:02.98 AFV.select :holdingCoil @21:56:03.09 delay 1 second @21:56:04.12 AS.reconfigure ASslow0 @21:56:04.17 AS.seek :empty @21:57:45.12 AFV.select :carrierSolution @21:57:45.24 delay 2 seconds @21:57:47.27 AS.reconfigure ASconfig @21:57:47.32 AS.seek 1ml @21:58:45.13 delay 1 second @21:58:46.14 AFV.select :holdingCoil @21:58:46.25 delay 1 second @21:58:47.28 AS.reconfigure ASslow0 @21:58:47.32 AS.seek :empty @22:00:28.31 AFV.select :carrierSolution @22:00:28.43 delay 2 seconds @22:00:30.46 AS.reconfigure ASconfig @22:00:30.51 AS.seek 1ml @22:01:28.32 delay 1 second @22:01:29.33 AFV.select :holdingCoil @22:01:29.44 delay 1 second @22:01:30.47 AS.reconfigure ASslow0 @22:01:30.52 AS.seek :empty @22:03:11.46 ATV.dial :air2 @22:03:12.11 AS.reconfigure ASslow1 @22:03:12.16 AS.seek 0.009ml @22:03:14.52 AS.reconfigure ASslow0 @22:03:14.57 AS.seek 1ml @22:04:54.53 ATV.dial AIV @22:04:55.36 AS.seek 0.02ml @22:06:34.23 ATV.dial :waste @22:06:35.87 AS.seek 0ml @22:06:38.17 AWV.select :air @22:06:38.34 Cleaning PCR @22:06:38.35 AFV.select :carrierSolution @22:06:38.46 delay 2 seconds @22:06:40.49 AS.reconfigure ASconfig @22:06:40.54 AS.seek 0.5ml @22:07:09.57 delay 1 second @22:07:10.58 AFV.select :holdingCoil @22:07:10.69 ASV.dial :waste1 @22:07:11.05 ATV.dial :air3 @22:07:11.72 AS.reconfigure ASslow1 @22:07:11.77 AS.seek 0.51ml @22:07:14.08 delay 2 seconds @22:07:16.09 ATV.dial :bleach @22:07:16.54 AS.seek 0.81ml @22:08:17.15 delay 3 seconds @22:08:20.17 ATV.dial :air3 @22:08:20.69 delay 3 seconds @22:08:23.78 AS.seek 0.835ml @22:08:29.05 delay 3 seconds @22:08:32.07 ATV.dial :bleach @22:08:32.67 AS.seek 0.82ml @22:08:36.01 delay 3 seconds @22:08:39.03 ARV.dial :waste @22:08:39.63 delay 3 seconds @22:08:42.65 ATV.dial :ARV @22:08:43.58 AS.reconfigure ASconfig @22:08:43.64 AS.seek 0.47ml @22:09:04.06 AFV.select :carrierSolution @22:09:04.18 delay 2 seconds @22:09:06.21 AS.seek 1ml @22:09:36.94 delay 1 second @22:09:37.95 AFV.select :holdingCoil @22:09:38.06 delay 1 second @22:09:39.09 AS.reconfigure ASfast @22:09:39.14 AS.seek 0ml @22:10:06.47 AFV.select :carrierSolution @22:10:06.59 delay 2 seconds @22:10:08.62 AS.reconfigure ASconfig @22:10:08.67 AS.seek 1ml @22:11:06.46 delay 1 second @22:11:07.47 AFV.select :holdingCoil @22:11:07.58 delay 1 second @22:11:08.61 AS.reconfigure ASfast @22:11:08.66 AS.seek 0ml @22:11:36.00 AFV.select :carrierSolution @22:11:36.12 delay 2 seconds @22:11:38.15 AS.reconfigure ASconfig @22:11:38.20 AS.seek 1ml @22:12:35.93 delay 1 second @22:12:36.94 AFV.select :holdingCoil @22:12:37.05 delay 1 second @22:12:38.08 AS.reconfigure ASfast @22:12:38.13 AS.seek 0ml @22:13:05.48 ARV.dial between carrier and air1 @22:13:05.98 ATV.dial :air3 @22:13:07.88 AS.reconfigure ASslow1 @22:13:07.93 AS.seek 0.01ml @22:13:10.35 delay 2 seconds @22:13:12.36 ATV.dial :bleach @22:13:12.98 AS.seek 0.81ml @22:15:54.18 delay 3 seconds @22:15:57.20 ATV.dial :air3 @22:15:57.73 delay 3 seconds @22:16:00.82 AS.seek 0.835ml @22:16:06.10 delay 3 seconds @22:16:09.13 ATV.dial :bleach @22:16:09.72 AS.seek 0.82ml @22:16:13.01 delay 3 seconds @22:16:16.03 ATV.dial PCR @22:16:16.41 AS.reconfigure ASconfig @22:16:16.46 AS.seek :empty @22:17:03.92 PCR.program :bleachProfile @22:17:03.98 PCR program should complete in < 2:45 @22:17:03.99 PCR.run 10:00 @22:20:23.99 PCR.off @22:20:24.02 AFV.select :carrierSolution @22:20:24.13 delay 2 seconds @22:20:26.16 AS.seek 1ml @22:21:23.99 delay 1 second @22:21:25.00 AFV.select :holdingCoil @22:21:25.11 delay 1 second @22:21:26.14 AS.reconfigure ASfast @22:21:26.19 AS.seek 0ml @22:21:53.54 delay 3 seconds @22:21:56.56 AFV.select :carrierSolution @22:21:56.67 delay 2 seconds @22:21:58.70 AS.reconfigure ASconfig @22:21:58.75 AS.seek 1ml @22:22:56.56 delay 1 second @22:22:57.57 AFV.select :holdingCoil @22:22:57.68 delay 1 second @22:22:58.71 AS.reconfigure ASfast @22:22:58.76 AS.seek 0ml @22:23:26.12 delay 3 seconds @22:23:29.14 AFV.select :carrierSolution @22:23:29.25 delay 2 seconds @22:23:31.28 AS.reconfigure ASconfig @22:23:31.34 AS.seek 1ml @22:24:29.09 delay 1 second @22:24:30.10 AFV.select :holdingCoil @22:24:30.21 delay 1 second @22:24:31.24 AS.reconfigure ASfast @22:24:31.30 AS.seek 0ml @22:24:58.65 delay 3 seconds @22:25:01.67 AFV.select :carrierSolution @22:25:01.78 delay 2 seconds @22:25:03.81 AS.reconfigure ASconfig @22:25:03.87 AS.seek 1ml @22:26:01.67 delay 1 second @22:26:02.69 AFV.select :holdingCoil @22:26:02.80 delay 1 second @22:26:03.83 AS.reconfigure ASfast @22:26:03.88 AS.seek 0ml @22:26:31.23 delay 3 seconds @22:26:34.26 AFV.select :carrierSolution @22:26:34.36 delay 2 seconds @22:26:36.39 AS.reconfigure ASconfig @22:26:36.46 AS.seek 1ml @22:27:34.26 delay 1 second @22:27:35.27 AFV.select :holdingCoil @22:27:35.38 delay 1 second @22:27:36.41 AS.reconfigure ASnorm @22:27:36.46 AS.seek 0ml @22:28:27.15 AFV.select :carrierSolution @22:28:27.26 delay 2 seconds @22:28:29.29 AS.reconfigure ASconfig @22:28:29.34 AS.seek 1ml @22:29:27.12 delay 1 second @22:29:28.13 AFV.select :holdingCoil @22:29:28.24 delay 1 second @22:29:29.26 ATV.dial :waste @22:29:30.40 AS.reconfigure ASnorm @22:29:30.46 AS.seek 0.5ml @22:29:56.00 ATV.dial :waste2 @22:29:57.20 AS.seek 0ml @22:30:22.75 AS.coast @22:30:22.79 SS.coast @22:30:22.83 CC.coast @22:30:22.87 CS.coast @22:30:22.91 PC.coast @22:30:22.95 PS.coast @22:30:22.99 Forearm.coast @22:30:23.03 Elbow.coast @22:30:23.43 Gate.power :core,:OFF @22:30:23.44 Gate.power :raw,:OFF @22:30:23.45 Shutdown @22:30:23.47 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @08:45:34.25CEST16-Oct-18 Session Begun @08:45:46.18 -> can @08:53:40.27 -> exit @08:53:40.29 Session Ended @09:01:31.14 Session Begun @09:01:35.49 -> exit @09:01:35.51 Session Ended @09:58:46.99 Session Begun @09:58:57.96 -> ESP.wakeup @09:58:58.01 Powering core bus @09:59:03.04 Gate.power :raw,:ON @09:59:03.74 SPE.coast @09:59:03.83 SPE.configure SPEconfig @09:59:03.95 AS.configure ASconfig @09:59:04.08 SS.configure SSconfig @09:59:04.20 CC.configure CCconfig @09:59:04.32 CS.configure CSconfig @09:59:04.45 PC.configure PCconfig @09:59:04.58 PS.configure PSconfig @09:59:04.73 Forearm.configure LooseForearm @09:59:04.85 Elbow.configure LooseElbow @09:59:05.00 SC.configure SCloose @09:59:05.13 SE.configure SEconfig @09:59:05.20 CH.coast @09:59:05.27 CH.configure CHconfig @09:59:05.33 PH.coast @09:59:05.42 PH.configure PHconfig @09:59:05.49 PCR.off @09:59:05.54 PCR.pidtemp= 50,0.1,0,300,25,25 @09:59:05.59 PCR.caltemp= 2.7073,0.0373,0 @09:59:05.64 All dwarf controllers running firmware version 3.75 @09:59:05.78 Valve.reset @09:59:05.97 PCR.off @09:59:06.02 PCR.pidtemp= 50,0.1,0,300,25,25 @09:59:06.07 PCR.caltemp= 2.7073,0.0373,0 @09:59:06.20 Intake.select :close @09:59:06.28 Exhaust.select :close @09:59:06.33 CTV.dial :air @09:59:06.44 PSV.dial :air @09:59:06.47 CSV.dial :waste1 @09:59:06.67 SSV.dial :spit,avoiding: :exhaust @09:59:06.69 Forearm.reconfigure LooseForearm @09:59:06.81 Forearm.home.seek :retract @09:59:07.06 PS.reconfigure PSconfig @09:59:07.13 PS.home.seek 0ml @09:59:07.60 Hand.close @09:59:07.63 Elbow.jog 1500 @09:59:07.79 CS.reconfigure CSfast @09:59:07.83 SS.reconfigure SSconfig @09:59:07.93 CS.home.seek 0ml @09:59:07.97 SS.home.seek 0ml @09:59:08.21 Elbow.reconfigure LooseElbow @09:59:08.29 Elbow.home.seek :clear @09:59:11.76 SE.reconfigure SEconfig @09:59:11.83 SE.home.seek :down @09:59:12.55 SC.reconfigure SCsloppy @09:59:12.62 SC.home.seek :initial @09:59:16.16 PS.coast @09:59:16.75 Forearm.seek :retract @09:59:16.87 Elbow.seek :hammer @09:59:18.11 Forearm.reconfigure TightForearm @09:59:18.19 Forearm.seek 5 + 350 counts @09:59:19.28 Forearm.seek :retract @09:59:20.50 PC.reconfigure PCconfig @09:59:21.01 CC.reconfigure CCconfig @09:59:21.21 PC.home.seek :open @09:59:21.30 CC.home.seek :open @09:59:45.79 -> MFB.ready! @09:59:45.90 Readying the MFB @09:59:45.92 Homing MFB Syringe @09:59:45.94 AFV.select :holdingCoil @09:59:46.12 ATV.dial :air2 @09:59:46.66 AS.reconfigure ASconfig @09:59:46.74 AS.home.seek :home @09:59:52.29 AFV.select :carrierSolution @09:59:52.43 delay 2 seconds @09:59:54.46 AS.seek 1ml @10:00:47.36 delay 1 second @10:00:48.37 AFV.select :holdingCoil @10:00:48.50 delay 1 second @10:00:49.53 ATV.dial :waste @10:00:50.63 AS.reconfigure ASnorm @10:00:50.70 AS.home.seek :empty @10:01:43.32 ATV.dial :waste @10:01:43.34 ARV.dial between carrier and air1 @10:01:43.47 ASV.dial :waste1 @10:04:33.08 -> ATV.ARV @10:04:33.10 ATV.dial ARV @10:04:41.40 -> Tt1 @10:05:12.84 -> ARV.legend @10:05:29.40 -> ARV.air2 @10:05:29.43 ARV.dial :air2 @10:05:36.76 -> AS @10:06:12.82 -> AS.pull 0.05 ASslow1 @10:06:12.84 SyntaxError in bkha -- compile error bkha:8: syntax error, unexpected tCONSTANT, expecting $end AS.pull 0.05 ASslow1 ^ @10:06:27.24 -> AS.pull 0.05 AS.slow1 @10:06:27.26 SyntaxError in bkha -- compile error bkha:9: syntax error, unexpected tCONSTANT, expecting $end AS.pull 0.05 AS.slow1 ^ @10:07:09.03 -> AS.pull 0.05, ASslow1 @10:07:09.13 AS.reconfigure ASslow1 @10:07:09.20 AS.seek 0.049ml @10:08:58.56 -> ARV.PP4 @10:08:58.59 ARV.dial :PP4 @10:09:54.52 -> AS.pull 0.015, ASslow2 @10:09:54.63 AS.reconfigure ASslow2 @10:09:54.71 AS.seek 0.064ml @10:10:30.38 -> ARV.air2 @10:10:30.41 ARV.dial :air2 @10:10:59.18 -> ATV @10:11:51.72 -> AS.pull 0.05, ASslow1 @10:11:51.83 AS.reconfigure ASslow1 @10:11:51.90 AS.seek 0.114ml @10:12:16.72 -> ARV.MM2 @10:12:16.74 ARV.dial :MM2 @10:12:45.82 -> AS.pull 0.015, ASslow2 @10:12:45.93 AS.reconfigure ASslow2 @10:12:46.01 AS.seek 0.129ml @10:13:32.29 -> ARV.air2 @10:13:32.32 ARV.dial :air2 @10:13:42.31 -> AS.pull 0.05, ASslow1 @10:13:42.42 AS.reconfigure ASslow1 @10:13:42.50 AS.seek 0.179ml @10:18:15.05 -> pcrDecon @10:18:15.14 Cleaning PCR @10:18:15.16 AFV.select :carrierSolution @10:18:15.30 delay 2 seconds @10:18:17.33 AS.reconfigure ASconfig @10:18:17.40 AS.seek 0.5ml @10:18:36.17 delay 1 second @10:18:37.18 AFV.select :holdingCoil @10:18:37.31 ASV.dial :waste1 @10:18:37.33 ATV.dial :air3 @10:18:38.56 AS.reconfigure ASslow1 @10:18:38.64 AS.seek 0.51ml @10:18:40.92 delay 2 seconds @10:18:42.94 ATV.dial :bleach @10:18:43.57 AS.seek 0.81ml @10:19:44.17 delay 3 seconds @10:19:47.19 ATV.dial :air3 @10:19:47.75 delay 3 seconds @10:19:50.89 AS.seek 0.835ml @10:19:56.23 delay 3 seconds @10:19:59.25 ATV.dial :bleach @10:19:59.86 AS.seek 0.82ml @10:20:03.14 delay 3 seconds @10:20:06.16 ARV.dial :waste @10:20:07.59 delay 3 seconds @10:20:10.61 ATV.dial :ARV @10:20:11.46 AS.reconfigure ASconfig @10:20:11.54 AS.seek 0.47ml @10:20:31.95 AFV.select :carrierSolution @10:20:32.10 delay 2 seconds @10:20:34.13 AS.seek 1ml @10:21:04.90 delay 1 second @10:21:05.91 AFV.select :holdingCoil @10:21:06.04 delay 1 second @10:21:07.07 AS.reconfigure ASfast @10:21:07.14 AS.seek 0ml @10:21:35.05 AFV.select :carrierSolution @10:21:35.19 delay 2 seconds @10:21:37.22 AS.reconfigure ASconfig @10:21:37.29 AS.seek 1ml @10:22:35.11 delay 1 second @10:22:36.12 AFV.select :holdingCoil @10:22:36.25 delay 1 second @10:22:37.28 AS.reconfigure ASfast @10:22:37.35 AS.seek 0ml @10:23:04.71 AFV.select :carrierSolution @10:23:04.86 delay 2 seconds @10:23:06.89 AS.reconfigure ASconfig @10:23:06.96 AS.seek 1ml @10:24:04.78 delay 1 second @10:24:05.79 AFV.select :holdingCoil @10:24:05.93 delay 1 second @10:24:06.96 AS.reconfigure ASfast @10:24:07.03 AS.seek 0ml @10:24:34.39 ARV.dial between carrier and air1 @10:24:34.90 ATV.dial :air3 @10:24:36.11 AS.reconfigure ASslow1 @10:24:36.19 AS.seek 0.01ml @10:24:38.58 delay 2 seconds @10:24:40.59 ATV.dial :bleach @10:24:41.22 AS.seek 0.81ml @10:27:22.37 delay 3 seconds @10:27:25.39 ATV.dial :air3 @10:27:25.94 delay 3 seconds @10:27:29.03 AS.seek 0.835ml @10:27:34.35 delay 3 seconds @10:27:37.37 ATV.dial :bleach @10:27:37.99 AS.seek 0.82ml @10:27:41.33 delay 3 seconds @10:27:44.35 ATV.dial PCR @10:27:44.76 AS.reconfigure ASconfig @10:27:44.83 AS.seek :empty @10:28:32.30 PCR.program :bleachProfile @10:28:32.38 PCR program should complete in < 2:45 @10:28:32.40 PCR.run 10:00 @10:32:04.55 PCR.off @10:32:04.59 AFV.select :carrierSolution @10:32:04.74 delay 2 seconds @10:32:06.77 AS.seek 1ml @10:33:04.62 delay 1 second @10:33:05.63 AFV.select :holdingCoil @10:33:05.76 delay 1 second @10:33:06.79 AS.reconfigure ASfast @10:33:06.86 AS.seek 0ml @10:33:34.23 delay 3 seconds @10:33:37.25 AFV.select :carrierSolution @10:33:37.39 delay 2 seconds @10:33:39.42 AS.reconfigure ASconfig @10:33:39.50 AS.seek 1ml @10:34:37.30 delay 1 second @10:34:38.31 AFV.select :holdingCoil @10:34:38.44 delay 1 second @10:34:39.47 AS.reconfigure ASfast @10:34:39.55 AS.seek 0ml @10:35:06.92 delay 3 seconds @10:35:09.94 AFV.select :carrierSolution @10:35:10.08 delay 2 seconds @10:35:12.11 AS.reconfigure ASconfig @10:35:12.18 AS.seek 1ml @10:36:09.97 delay 1 second @10:36:10.98 AFV.select :holdingCoil @10:36:11.11 delay 1 second @10:36:12.14 AS.reconfigure ASfast @10:36:12.21 AS.seek 0ml @10:36:39.60 delay 3 seconds @10:36:42.62 AFV.select :carrierSolution @10:36:42.76 delay 2 seconds @10:36:44.79 AS.reconfigure ASconfig @10:36:44.87 AS.seek 1ml @10:37:42.69 delay 1 second @10:37:43.70 AFV.select :holdingCoil @10:37:43.83 delay 1 second @10:37:44.86 AS.reconfigure ASfast @10:37:44.93 AS.seek 0ml @10:38:12.31 delay 3 seconds @10:38:15.33 AFV.select :carrierSolution @10:38:15.47 delay 2 seconds @10:38:17.50 AS.reconfigure ASconfig @10:38:17.58 AS.seek 1ml @10:39:15.38 delay 1 second @10:39:16.41 AFV.select :holdingCoil @10:39:16.54 delay 1 second @10:39:17.57 AS.reconfigure ASnorm @10:39:17.64 AS.seek 0ml @10:58:45.08 Session Ended @11:35:08.18 Session Begun @11:35:38.38 -> pcrDecon @11:35:38.48 Cleaning PCR @11:35:38.49 AFV.select :carrierSolution @11:35:38.64 delay 2 seconds @11:35:40.67 AS.reconfigure ASconfig @11:35:40.74 AS.seek 0.5ml @11:36:09.79 delay 1 second @11:36:10.80 AFV.select :holdingCoil @11:36:10.93 ASV.dial :waste1 @11:36:10.95 ATV.dial :air3 @11:36:11.75 AS.reconfigure ASslow1 @11:36:11.83 AS.seek 0.51ml @11:36:14.12 delay 2 seconds @11:36:16.13 ATV.dial :bleach @11:36:16.75 AS.seek 0.81ml @11:37:17.40 delay 3 seconds @11:37:20.42 ATV.dial :air3 @11:37:20.98 delay 3 seconds @11:37:24.06 AS.seek 0.835ml @11:37:29.39 delay 3 seconds @11:37:32.87 ATV.dial :bleach @11:37:33.58 AS.seek 0.82ml @11:37:36.88 delay 3 seconds @11:37:39.90 ARV.dial :waste @11:37:40.40 delay 3 seconds @11:37:43.42 ATV.dial :ARV @11:37:44.28 AS.reconfigure ASconfig @11:37:44.35 AS.seek 0.47ml @11:38:04.76 AFV.select :carrierSolution @11:38:04.90 delay 2 seconds @11:38:06.93 AS.seek 1ml @11:38:37.70 delay 1 second @11:38:38.72 AFV.select :holdingCoil @11:38:38.85 delay 1 second @11:38:39.88 AS.reconfigure ASfast @11:38:39.95 AS.seek 0ml @11:39:07.33 AFV.select :carrierSolution @11:39:07.48 delay 2 seconds @11:39:09.51 AS.reconfigure ASconfig @11:39:09.58 AS.seek 1ml @11:40:07.38 delay 1 second @11:40:08.39 AFV.select :holdingCoil @11:40:08.52 delay 1 second @11:40:09.55 AS.reconfigure ASfast @11:40:09.62 AS.seek 0ml @11:40:36.97 AFV.select :carrierSolution @11:40:37.11 delay 2 seconds @11:40:39.14 AS.reconfigure ASconfig @11:40:39.21 AS.seek 1ml @11:41:37.01 delay 1 second @11:41:38.02 AFV.select :holdingCoil @11:41:38.15 delay 1 second @11:41:39.18 AS.reconfigure ASfast @11:41:39.25 AS.seek 0ml @11:42:06.61 ARV.dial between carrier and air1 @11:42:07.12 ATV.dial :air3 @11:42:08.35 AS.reconfigure ASslow1 @11:42:08.43 AS.seek 0.01ml @11:42:10.81 delay 2 seconds @11:42:12.83 ATV.dial :bleach @11:42:13.45 AS.seek 0.81ml @11:44:54.64 delay 3 seconds @11:44:57.66 ATV.dial :air3 @11:44:58.21 delay 3 seconds @11:45:01.30 AS.seek 0.835ml @11:45:06.61 delay 3 seconds @11:45:09.63 ATV.dial :bleach @11:45:10.25 AS.seek 0.82ml @11:45:13.56 delay 3 seconds @11:45:16.58 ATV.dial PCR @11:45:16.98 AS.reconfigure ASconfig @11:45:17.05 AS.seek :empty @11:46:04.56 PCR.program :bleachProfile @11:46:04.64 PCR program should complete in < 2:45 @11:46:04.65 PCR.run 10:00 @11:49:30.74 PCR.off @11:49:30.78 AFV.select :carrierSolution @11:49:30.91 delay 2 seconds @11:49:32.94 AS.seek 1ml @11:50:30.81 delay 1 second @11:50:31.83 AFV.select :holdingCoil @11:50:31.96 delay 1 second @11:50:32.99 AS.reconfigure ASfast @11:50:33.06 AS.seek 0ml @11:51:00.42 delay 3 seconds @11:51:03.44 AFV.select :carrierSolution @11:51:03.58 delay 2 seconds @11:51:05.61 AS.reconfigure ASconfig @11:51:05.69 AS.seek 1ml @11:52:03.51 delay 1 second @11:52:04.52 AFV.select :holdingCoil @11:52:04.65 delay 1 second @11:52:05.68 AS.reconfigure ASfast @11:52:05.75 AS.seek 0ml @11:52:33.14 delay 3 seconds @11:52:36.16 AFV.select :carrierSolution @11:52:36.30 delay 2 seconds @11:52:38.33 AS.reconfigure ASconfig @11:52:38.41 AS.seek 1ml @11:53:36.22 delay 1 second @11:53:37.23 AFV.select :holdingCoil @11:53:37.36 delay 1 second @11:53:38.39 AS.reconfigure ASfast @11:53:38.46 AS.seek 0ml @11:54:05.84 delay 3 seconds @11:54:08.86 AFV.select :carrierSolution @11:54:09.00 delay 2 seconds @11:54:11.03 AS.reconfigure ASconfig @11:54:11.10 AS.seek 1ml @11:55:08.92 delay 1 second @11:55:09.93 AFV.select :holdingCoil @11:55:10.06 delay 1 second @11:55:11.09 AS.reconfigure ASfast @11:55:11.16 AS.seek 0ml @11:55:39.12 delay 3 seconds @11:55:42.14 AFV.select :carrierSolution @11:55:42.28 delay 2 seconds @11:55:44.31 AS.reconfigure ASconfig @11:55:44.39 AS.seek 1ml @11:56:42.19 delay 1 second @11:56:43.20 AFV.select :holdingCoil @11:56:43.33 delay 1 second @11:56:44.36 AS.reconfigure ASnorm @11:56:44.46 AS.seek 0ml @12:05:11.70 -> ESP.shutdown @12:05:11.73 AS.coast @12:05:11.77 Asleep / Shutdown @12:05:11.84 SS.coast @12:05:11.90 CC.coast @12:05:11.96 CS.coast @12:05:12.01 PC.coast @12:05:12.07 Forearm.coast @12:05:12.13 Elbow.coast @12:05:12.19 SC.coast @12:05:12.80 SE.coast @12:05:13.22 Gate.power :core,:OFF @12:05:13.24 Gate.power :raw,:OFF @12:05:13.26 Shutdown @12:05:15.95 -> exit @12:05:15.96 Session Ended @14:01:18.99CEST17-Oct-18
Powering core bus @14:01:23.81 Gate.power :raw,:ON @14:01:24.47 SPE.coast @14:01:24.54 SPE.configure SPEconfig @14:01:24.63 AS.configure ASconfig @14:01:24.73 SS.configure SSconfig @14:01:24.83 CC.configure CCconfig @14:01:24.93 CS.configure CSconfig @14:01:25.02 PC.configure PCconfig @14:01:25.12 PS.configure PSconfig @14:01:25.24 Forearm.configure LooseForearm @14:01:25.34 Elbow.configure LooseElbow @14:01:25.46 SC.configure SCloose @14:01:25.55 SE.configure SEconfig @14:01:25.60 CH.coast @14:01:25.65 CH.configure CHconfig @14:01:25.69 PH.coast @14:01:25.75 PH.configure PHconfig @14:01:25.79 PCR.off @14:01:25.82 PCR.pidtemp= 50,0.1,0,300,25,25 @14:01:25.85 PCR.caltemp= 2.7073,0.0373,0 @14:01:25.88 All dwarf controllers running firmware version 3.75 @14:01:26.00 Valve.reset @14:01:26.17 PCR.off @14:01:26.21 PCR.pidtemp= 50,0.1,0,300,25,25 @14:01:26.23 PCR.caltemp= 2.7073,0.0373,0 @14:01:26.38 Intake.select :close @14:01:26.46 Exhaust.select :close @14:01:26.48 CTV.dial :air @14:01:26.57 PSV.dial :air @14:01:26.60 CSV.dial :waste1 @14:01:26.81 SSV.dial :spit,avoiding: :exhaust @14:01:26.84
Forearm.reconfigure LooseForearm @14:01:26.86 PS.reconfigure PSconfig @14:01:27.00 PS.home.seek 0ml @14:01:27.02
Forearm.home.seek :retract @14:01:27.22 CS.reconfigure CSfast @14:01:27.25 SS.reconfigure SSconfig @14:01:27.35 CS.home.seek 0ml @14:01:27.38 SS.home.seek 0ml @14:01:27.78 Hand.close @14:01:27.81
Elbow.jog 1500 @14:01:28.93 Elbow.reconfigure LooseElbow @14:01:28.98 Elbow.home.seek :clear @14:01:33.66 SE.reconfigure SEconfig @14:01:33.71 SE.home.seek :down @14:01:34.44 SC.reconfigure SCsloppy @14:01:34.49 SC.home.seek :initial @14:01:36.10 PS.coast @14:01:38.50
Forearm.seek :retract @14:01:38.58 Elbow.seek :hammer @14:01:39.91 Forearm.reconfigure TightForearm @14:01:39.96 Forearm.seek 5 + 350 counts @14:01:41.05 Forearm.seek :retract @14:01:42.28 PC.reconfigure PCconfig @14:01:42.30
CC.reconfigure CCconfig @14:01:42.39 PC.home.seek :open @14:01:42.42
CC.home.seek :open @14:01:45.93 Phase [mbaPhase] @14:01:45.94 GoodNews.email "At 14:01:45 began in tube 3: mbaPhase\n/home/esp/esp2/mission/18tundeployv3.rb:123\n{1 => 22, 2 => 17, 3 => 5}\nCan@14:01:45, 13.4C, 71% humidity, 14.3psia, 13.082V, 0.777A, 0.777A avg, 10.17W",Subject: "Started 18tundeployv3 phase mbaPhase" @14:01:45.98CEST17-Oct-18
==================> STARTED Superphase (phase #4) <=== @14:01:45.99 Starting initialPurge @14:01:46.00 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:01:46.08 Hand.open @14:01:46.92 Elbow.reconfigure TightElbow @14:01:46.97 Elbow.seek FlushPuck::Garage @14:01:48.82 Elbow.reconfigure LooseElbow @14:01:48.93 Forearm.reconfigure LooseForearm @14:01:48.98 Forearm.seek :garage @14:01:49.99 Hand.close @14:01:50.81 Forearm.seek :retract @14:01:51.82 Elbow.reconfigure TightElbow @14:01:51.88 Elbow.seek CC @14:01:54.79 Elbow.reconfigure LooseElbow @14:01:54.90 Forearm.reconfigure TightForearm @14:01:54.95 Forearm.seek CC @14:01:56.31 CC.seek :closed @14:02:00.55 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.06 @14:02:00.63 Hand.open @14:02:01.35 Forearm.reconfigure LooseForearm @14:02:01.39 Forearm.seek :retract @14:02:02.36 Flushing the Collection Valve::Manifold @14:02:02.49 CSV.dial CSR @14:02:03.93 Pulling 5ml of CSR.flush @14:02:03.94 CSR.select :flush @14:02:04.07 CS.reconfigure CSconfig @14:02:04.11 CS.seek 5ml @14:02:17.81 delay 5 seconds @14:02:22.83 CSR.select :air @14:02:23.01 CS.reconfigure CSfast @14:02:23.06 CS.seek 7.5ml @14:02:30.08 CSV.dial :waste @14:02:31.47 CS.reconfigure CSconfig @14:02:31.52 CS.seek 0ml @14:02:51.62 Purging external Intake @14:02:51.75 CSV.dial :flush @14:02:52.25 CS.reconfigure CSfast @14:02:52.30 CS.seek 10ml @14:03:17.00 delay 5 seconds @14:03:22.03 CSV.dial CBV @14:03:22.07
CBV.dial CSV @14:03:22.87 CTV.dial :intake @14:03:23.68 Intake.select :open @14:03:23.81 CS.seek 0ml @14:03:49.33 Intake.select :close @14:03:49.45 CTV.dial :air @14:03:50.25 Flushing upper manifold @14:03:50.39 CSV.dial :flush @14:03:51.18 CS.seek 5ml @14:04:04.53 delay 5 seconds @14:04:09.56 CSV.dial :air @14:04:10.12 CS.seek 7ml @14:04:15.84 CSV.dial CBV @14:04:15.87
CBV.dial CSV @14:04:16.97 CTV.dial :air @14:04:16.99 CS.seek 0ml @14:04:34.34 delay 7 seconds @14:04:41.38 CS.seek 3ml @14:04:49.49 CSV.dial :waste @14:04:49.98 CS.seek 0ml @14:04:58.22 CSV.dial :flush @14:04:58.71 CS.seek 3ml @14:05:06.81 delay 5 seconds @14:05:11.83 CSV.dial :air @14:05:12.37 CS.seek 5ml @14:05:18.02 CSV.dial :air @14:05:18.05 CS.seek 0ml @14:05:30.85 delay 7 seconds @14:05:37.89 CS.seek 3ml @14:05:46.02 CSV.dial :waste @14:05:46.81 CS.seek 0ml @14:05:54.85 Garaging FlushPuck in Collection Clamp @14:05:54.93 Elbow.reconfigure TightElbow @14:05:54.98 Elbow.seek CC @14:05:55.24 Elbow.reconfigure LooseElbow @14:05:55.35 Forearm.seek CC @14:05:56.35 Hand.close @14:05:57.02 CC.seek :open @14:06:01.38 Forearm.seek :retract @14:06:02.38 Elbow.reconfigure TightElbow @14:06:02.43 Elbow.seek :garage @14:06:05.53 Elbow.reconfigure LooseElbow @14:06:05.63 Forearm.reconfigure TightForearm @14:06:05.67 Forearm.seek :garage @14:06:06.83 Hand.open @14:06:07.59 Forearm.reconfigure LooseForearm @14:06:07.63 Forearm.seek :retract @14:06:08.72 Starting mfb @14:06:08.74 pcrBAC sampling at most 150ml, wcr at most 1500ml @14:06:08.80 BACfans.email "Started mfb in tube 3 at 14:06:08CEST17-Oct-18\n{1 => 22, 2 => 17, 3 => 5}\nCan@14:01:45, 13.4C, 71% humidity, 14.3psia, 13.082V, 0.777A, 0.777A avg, 10.17W",Subject: "pcrBAC sampling at most 150ml, wcr at most 1500ml" @14:06:08.87
Loading sh1 puck from tube 3 into Collection Clamp @14:06:08.97 Readying the MFB @14:06:09.00 Homing MFB Syringe @14:06:09.04 AFV.select :holdingCoil @14:06:09.27 ATV.dial :air2 @14:06:09.31
SC.reconfigure SCtight @14:06:09.40 SC.seek 3 @14:06:10.72 AS.reconfigure ASconfig @14:06:10.77 AS.home.seek :home @14:06:11.42
SC.reconfigure SCloose @14:06:11.66 Elbow.reconfigure TightElbow @14:06:11.71 Elbow.seek 3 @14:06:13.87 Forearm.reconfigure TightForearm @14:06:13.93 Forearm.seek 3 + 700 counts @14:06:15.94 SE.reconfigure SEpick @14:06:16.01 SE.seek beamBreak @14:06:16.30 AFV.select :carrierSolution @14:06:16.42 delay 2 seconds @14:06:18.46 AS.seek 1ml @14:06:18.73
SE.configure SEconfig @14:06:18.92 Elbow.seek 3 @14:06:19.14 Forearm.seek 3 + 300 counts @14:06:19.38 Forearm.reconfigure LooseForearm @14:06:19.43 Hand.close @14:06:20.04 SE.seek :down @14:06:20.29
Forearm.seek :retract @14:06:21.24 Elbow.seek CC @14:06:22.57 SE.coast @14:06:22.98
Elbow.reconfigure LooseElbow @14:06:23.10 Forearm.reconfigure TightForearm @14:06:23.15 Forearm.seek CC @14:06:24.48 CC.seek :closed @14:06:28.75 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.10 @14:06:28.76 Hand.open @14:06:29.48 Forearm.reconfigure LooseForearm @14:06:29.53 Forearm.seek :retract @14:06:30.53 SC.coast @14:06:30.58 collectSample 150ml w/bubble point of 55psi @14:06:30.59 Priming 7ml @14:06:30.60 Intake.select :close @14:06:30.61 Exhaust.select :close @14:06:30.62 SS.hold @14:06:30.86 Calibrating pressure and temperature sensors @14:06:30.90 CTV.dial :air @14:06:30.93 SSV.dial :spit,avoiding: :exhaust @14:06:30.97 SS.reconfigure SSfast @14:06:31.03 SS.seek 5ml @14:06:33.75 SSV.dial CBV @14:06:33.78
CBV.dial SSV @14:06:35.21 delay 3.8 seconds @14:06:39.15 SSV.dial :spit @14:06:40.57 SS.seek 0ml @14:06:43.23 SSV.dial CBV @14:06:44.63 CTV.dial :intake @14:06:45.44 SSV.dial CBV @14:06:45.47
CBV.dial SSV @14:06:45.48 Allowing 40s for 7.0ml of remaining 7.0ml @14:06:45.50 Intake.select :open @14:06:47.67 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:06:47.69 SS.reconfigure SSinhale.with maxSpeed: 150 @14:06:47.74 SS.seek 7ml @14:06:50.95 Vacuum too high -- waiting up to 40s @14:06:52.59 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:06:52.61 SS.reconfigure SSinhale.with maxSpeed: 135 @14:06:52.67 SS.seek 7ml @14:06:55.33 Vacuum too high -- waiting up to 40s @14:06:56.96 SS.reconfigure SSinhale.with maxSpeed: 122 @14:06:57.02 SS.seek 7ml @14:06:59.37 Stroke completed -- waiting for pressure to equalize ... @14:07:01.97 Intake.select :close @14:07:02.12 SSV.dial :exhaust @14:07:02.95 Exhaust.select :open @14:07:03.07 SS.reconfigure SSexhale @14:07:03.12 SS.seek 0ml @14:07:06.71 Exhaust.select :close @14:07:06.83 Sampling 150.0ml @14:07:06.84 Intake.select :close @14:07:06.85 Exhaust.select :close @14:07:06.87 SS.reconfigure SSconfig @14:07:06.91 SS.hold @14:07:07.13 CTV.dial :intake @14:07:07.15 SSV.dial CBV @14:07:07.18
CBV.dial SSV @14:07:08.03 Allowing 130s for 25.0ml of remaining 150.0ml @14:07:08.04 Intake.select :open @14:07:10.21 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:07:10.23 SS.reconfigure SSinhale.with maxSpeed: 122 @14:07:10.28 SS.seek 25ml @14:07:11.37 delay 1 second @14:07:12.39 AFV.select :holdingCoil @14:07:12.50 delay 1 second @14:07:13.52 ATV.dial :waste @14:07:14.20
Vacuum too high -- waiting up to 40s @14:07:14.52 AS.reconfigure ASnorm @14:07:14.57 AS.home.seek :empty @14:07:15.80
Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:07:15.82 SS.reconfigure SSinhale.with maxSpeed: 110 @14:07:15.87 SS.seek 25ml @14:07:18.54 Vacuum too high -- waiting up to 40s @14:07:20.12 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:07:20.14 SS.reconfigure SSinhale.with maxSpeed: 99 @14:07:20.19 SS.seek 25ml @14:07:22.70 Vacuum too high -- waiting up to 40s @14:07:24.30 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:07:24.32 SS.reconfigure SSinhale.with maxSpeed: 89 @14:07:24.37 SS.seek 25ml @14:07:27.14 Vacuum too high -- waiting up to 40s @14:07:28.54 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:07:28.56 SS.reconfigure SSinhale.with maxSpeed: 80 @14:07:28.61 SS.seek 25ml @14:07:31.61 Vacuum too high -- waiting up to 40s @14:07:32.96 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:07:32.97 SS.reconfigure SSinhale.with maxSpeed: 72 @14:07:33.02 SS.seek 25ml @14:07:36.04 Vacuum too high -- waiting up to 40s @14:07:37.40 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:07:37.42 SS.reconfigure SSinhale.with maxSpeed: 65 @14:07:37.47 SS.seek 25ml @14:07:41.27 Vacuum too high -- waiting up to 40s @14:07:42.35 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:07:42.37 SS.reconfigure SSinhale.with maxSpeed: 59 @14:07:42.42 SS.seek 25ml @14:07:46.21 Vacuum too high -- waiting up to 40s @14:07:47.30 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:07:47.32 SS.reconfigure SSinhale.with maxSpeed: 53 @14:07:47.37 SS.seek 25ml @14:07:53.15 Stroke completed -- waiting for pressure to equalize ... @14:07:56.36 Intake.select :close @14:07:56.51 SSV.dial :exhaust @14:07:57.34 Exhaust.select :open @14:07:57.46 SS.reconfigure SSexhale @14:07:57.51 SS.seek 0ml @14:08:06.63 Exhaust.select :close @14:08:06.75 SSV.dial CBV @14:08:06.78
CBV.dial SSV @14:08:07.12 ATV.dial :waste @14:08:07.14 ARV.dial between carrier and air1 @14:08:07.24 ASV.dial :waste1 @14:08:07.62
Allowing 130s for 25.0ml of remaining 125.0ml @14:08:07.64 Intake.select :open @14:08:09.81 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:08:09.83 SS.reconfigure SSinhale.with maxSpeed: 53 @14:08:09.88 SS.seek 25ml @14:08:16.64 Vacuum too high -- waiting up to 40s @14:08:17.71 Intake @33.6psia, vacuum from 20.0 to 24.0psi @14:08:17.72 SS.reconfigure SSinhale.with maxSpeed: 48 @14:08:17.77 SS.seek 25ml @14:08:28.92 Vacuum too high -- waiting up to 40s @14:08:30.29 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:08:30.31 SS.reconfigure SSinhale.with maxSpeed: 43 @14:08:30.36 SS.seek 25ml @14:08:52.34 Stroke completed -- waiting for pressure to equalize ... @14:08:55.55 Intake.select :close @14:08:55.71 SSV.dial :exhaust @14:08:56.53 Exhaust.select :open @14:08:56.65 SS.reconfigure SSexhale @14:08:56.71 SS.seek 0ml @14:09:05.68 Exhaust.select :close @14:09:05.80 SSV.dial CBV @14:09:05.85
CBV.dial SSV @14:09:06.66 Allowing 130s for 25.0ml of remaining 100.0ml @14:09:06.67 Intake.select :open @14:09:08.84 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:09:08.86 SS.reconfigure SSinhale.with maxSpeed: 43 @14:09:08.91 SS.seek 25ml @14:09:50.34 Stroke completed -- waiting for pressure to equalize ... @14:09:53.54 Intake.select :close @14:09:53.69 SSV.dial :exhaust @14:09:54.49 Exhaust.select :open @14:09:54.61 SS.reconfigure SSexhale @14:09:54.66 SS.seek 0ml @14:10:03.79 Exhaust.select :close @14:10:03.91 SSV.dial CBV @14:10:03.94
CBV.dial SSV @14:10:04.77 Allowing 130s for 25.0ml of remaining 75.0ml @14:10:04.78 Intake.select :open @14:10:06.95 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:10:06.97 SS.reconfigure SSinhale.with maxSpeed: 43 @14:10:07.02 SS.seek 25ml @14:10:44.04 Vacuum too high -- waiting up to 40s @14:10:44.87 Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:10:44.89 SS.reconfigure SSinhale.with maxSpeed: 39 @14:10:44.94 SS.seek 25ml @14:10:49.67 Stroke completed -- waiting for pressure to equalize ... @14:10:52.24 Intake.select :close @14:10:52.39 SSV.dial :exhaust @14:10:53.20 Exhaust.select :open @14:10:53.32 SS.reconfigure SSexhale @14:10:53.37 SS.seek 0ml @14:11:02.70 Exhaust.select :close @14:11:02.82 SSV.dial CBV @14:11:02.85
CBV.dial SSV @14:11:03.69 Allowing 130s for 25.0ml of remaining 50.0ml @14:11:03.70 Intake.select :open @14:11:05.87 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:11:05.89 SS.reconfigure SSinhale.with maxSpeed: 39 @14:11:05.94 SS.seek 25ml @14:11:51.63 Stroke completed -- waiting for pressure to equalize ... @14:11:54.21 Intake.select :close @14:11:54.36 SSV.dial :exhaust @14:11:55.18 Exhaust.select :open @14:11:55.30 SS.reconfigure SSexhale @14:11:55.35 SS.seek 0ml @14:12:04.51 Exhaust.select :close @14:12:04.63 SSV.dial CBV @14:12:05.22
CBV.dial SSV @14:12:06.05 Allowing 130s for 25.0ml of remaining 25.0ml @14:12:06.06 Intake.select :open @14:12:08.23 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:12:08.25 SS.reconfigure SSinhale.with maxSpeed: 39 @14:12:08.30 SS.seek 25ml @14:12:53.85 Stroke completed -- waiting for pressure to equalize ... @14:12:56.45 Intake.select :close @14:12:56.60 SSV.dial :exhaust @14:12:57.41 Exhaust.select :open @14:12:57.53 SS.reconfigure SSexhale @14:12:57.58 SS.seek 0ml @14:13:06.65 Exhaust.select :close @14:13:06.77 Sampled 150.0ml @14:13:06.79 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @14:13:06.86 CSV.dial :waste @14:13:06.94 CS.seek 0ml @14:13:07.04 SSV.dial CBV,avoiding: :exhaust @14:13:07.07
CBV.dial SSV @14:13:07.14 CSV.dial :air @14:13:07.94 CS.seek 10ml @14:13:09.08
CTV.dial :air @14:13:09.88 delay 15 seconds @14:13:24.91 SSV.dial :spit,avoiding: :exhaust @14:13:26.31 CTV.dial CSV,avoiding: :intake @14:13:26.36
SS.seek 0ml @14:13:26.49 SSV.dial CBV,avoiding: :exhaust @14:13:32.94 CBV.dial between SSV and waste2 @14:13:33.36 CTV.dial CSV,avoiding: :air @14:13:33.39
CSV.dial CTV @14:13:33.88 CS.reconfigure CSpressurize @14:13:33.93 CS.seek 0ml @14:13:59.99 BubblePoint: Intake @70.4psia, Outlet @14.7psia @14:14:00.00 CBV.dial SSV @14:14:00.35 SS.reconfigure SSconfig @14:14:00.40 SS.seek 10ml @14:14:06.03 delay 15 seconds @14:14:21.17 Evacuated: Intake @35.8psia, Outlet @1.2psia @14:14:21.19 SSV.dial :spit,avoiding: :exhaust @14:14:22.60 SS.reconfigure SSexhale @14:14:22.65 SS.seek 0ml @14:14:27.65 SS.reconfigure SSfast @14:14:27.70 SS.seek 5ml @14:14:30.61 SSV.dial CBV,avoiding: :exhaust @14:14:30.63
CBV.dial SSV @14:14:32.05 CTV.dial :air,avoiding: :intake @14:14:33.46 CSV.dial CTV @14:14:33.51
SS.seek 10ml @14:14:34.50 CSV.dial :air @14:14:34.99 CS.reconfigure CSfast @14:14:35.04 CS.seek 0ml @14:14:35.56 CS.coast @14:14:36.13
SSV.dial :spit,avoiding: :exhaust @14:14:37.55 SS.reconfigure SSexhale @14:14:37.60 SS.seek 0ml @14:14:42.87 SS.reconfigure SSfast @14:14:42.92 SS.seek 5ml @14:14:45.70 SSV.dial CBV,avoiding: :exhaust @14:14:45.73
CBV.dial SSV @14:14:48.15 SSV.dial :spit,avoiding: :exhaust @14:14:50.10 SS.reconfigure SSconfig @14:14:50.15 SS.seek 0ml @14:14:53.15 SS.coast @14:14:53.20 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @14:14:53.21 Intake.select :close @14:14:53.29 CSV.dial :waste @14:14:53.44 SSV.dial CBV,avoiding: :exhaust @14:14:53.47
CBV.dial SSV @14:14:54.15 CS.seek 0ml @14:14:54.22 CSV.dial :air @14:14:54.91
CTV.dial :air @14:14:54.92 delay 15 seconds @14:14:55.02 CS.seek 10ml @14:15:09.95
SSV.dial :spit,avoiding: :exhaust @14:15:11.36 CTV.dial CSV,avoiding: :intake @14:15:11.42
SS.reconfigure SSexhale @14:15:11.51 SS.seek 0ml @14:15:11.57 SSV.dial CBV,avoiding: :exhaust @14:15:19.66 CBV.dial between SSV and waste2 @14:15:20.10 CSV.dial between air and CTV @14:15:20.43 CS.seek 8.211ml @14:15:25.73 CTV.dial CSV,avoiding: :air @14:15:25.76
CSV.dial CTV @14:15:26.09 CS.reconfigure CSpressurize @14:15:26.14 CS.seek 0ml @14:15:47.78 CSV.dial :air @14:15:48.28 CS.reconfigure CSfast @14:15:48.33 CS.seek 10ml @14:16:12.76 CSV.dial between air and CTV @14:16:13.12 CS.seek 3.128ml @14:16:30.23 CTV.dial CSV,avoiding: :air @14:16:30.26
CSV.dial CTV @14:16:30.59 CS.reconfigure CSpressurize @14:16:30.65 CS.seek 0ml @14:16:38.93 BubblePoint: Intake @69.4psia, Outlet @14.1psia @14:16:38.94 CBV.dial SSV @14:16:39.29 SS.reconfigure SSconfig @14:16:39.34 SS.seek 10ml @14:16:44.67 delay 15 seconds @14:16:59.81 Evacuated: Intake @36.7psia, Outlet @17.0psia @14:16:59.82 SSV.dial :spit,avoiding: :exhaust @14:17:01.24 SS.reconfigure SSexhale @14:17:01.29 SS.seek 0ml @14:17:06.47 SS.reconfigure SSfast @14:17:06.52 SS.seek 5ml @14:17:09.06 SSV.dial CBV,avoiding: :exhaust @14:17:09.09
CBV.dial SSV @14:17:10.52 CTV.dial :air,avoiding: :intake @14:17:11.93 CSV.dial CTV @14:17:11.98
SS.seek 10ml @14:17:12.97 CSV.dial :air @14:17:13.47 CS.reconfigure CSfast @14:17:13.52 CS.seek 0ml @14:17:14.47
SSV.dial :spit,avoiding: :exhaust @14:17:14.57 CS.coast @14:17:15.89
SS.reconfigure SSexhale @14:17:15.95 SS.seek 0ml @14:17:20.95 SS.reconfigure SSfast @14:17:21.53 SS.seek 5ml @14:17:24.25 SSV.dial CBV,avoiding: :exhaust @14:17:24.28
CBV.dial SSV @14:17:26.71 SSV.dial :spit,avoiding: :exhaust @14:17:28.11 SS.reconfigure SSconfig @14:17:28.16 SS.seek 0ml @14:17:31.32 SS.coast @14:17:31.37 Lysing: Adding 1.5ml lysis @14:17:31.38 CBV.dial between CSV and waste1,avoiding: :DBV @14:17:32.98 CTV.dial CSV @14:17:34.39 CSV.dial CSR @14:17:35.24 Pulling 1ml of CSR.lysis @14:17:35.25 CSR.select :lysis @14:17:35.37 CS.reconfigure CSconfig @14:17:35.41 CS.seek 1ml @14:17:38.98 delay 5 seconds @14:17:44.00 CSR.select :air @14:17:44.19 CS.seek 6ml @14:17:57.98 delay 2 seconds @14:17:59.99 CSV.dial :waste1 @14:18:01.37 CS.reconfigure CSfast @14:18:01.42 CS.seek :empty @14:18:16.41 CSV.dial CSR @14:18:17.87 Pulling 1.5ml of CSR.lysis @14:18:17.88 CSR.select :lysis @14:18:17.99 CS.reconfigure CSconfig @14:18:18.04 CS.seek 1.5ml @14:18:22.86 delay 5 seconds @14:18:27.88 CSR.select :air @14:18:28.07 CS.seek 4.5ml @14:18:36.67 delay 2 seconds @14:18:38.68 CSV.dial CTV @14:18:39.23 CS.seek 1.275ml @14:18:48.45 Heating sh1 puck from tube 3 in Collection Clamp to 85C for 5:00 @14:18:48.47 CH.seek 85C,8:00 @14:21:58.68 CH.hold 5:00,85C @14:26:58.86 Collection Puck Heater at 85C after lysing @14:26:58.88 CS.seek 0ml @14:27:03.15 CTV.dial between spare and air,avoiding: :intake @14:27:04.27 CSV.dial :air @14:27:04.76 CS.seek 5ml @14:27:18.48 CSV.dial CTV @14:27:18.97 delay 2 seconds @14:27:20.98 CSV.dial :air @14:27:21.49 delay 2 seconds @14:27:23.50 CSV.dial CTV @14:27:23.99 delay 2 seconds @14:27:26.00 CSV.dial :air @14:27:26.49 delay 2 seconds @14:27:28.51 CS.seek 0ml @14:27:42.37 Recovering Lysate into Collection syringe @14:27:42.38 CSV.dial CBV @14:27:43.46 CBV.dial CSV @14:27:43.87 CS.seek 5ml @14:27:57.55 delay 5 seconds @14:28:02.57 CTV.dial :air @14:28:03.16 CS.seek 7ml @14:28:09.24 delay 5 seconds @14:28:14.26 CSV.dial :air2 @14:28:14.74 CS.seek 10ml @14:28:23.45 CSV.dial between air and CTV,avoiding: CBV @14:28:24.68 Lysing Complete @14:28:24.76 Storing sh1 puck in Collection Clamp to tube 2 @14:28:24.95 SC.reconfigure SCtight @14:28:25.00 SC.seek 2 @14:28:27.16 SC.reconfigure SCloose @14:28:27.38 Elbow.reconfigure TightElbow @14:28:27.43 Elbow.seek 2 @14:28:29.34 Forearm.reconfigure TightForearm @14:28:29.39 Forearm.seek 2 + 700 counts @14:28:30.47 SE.reconfigure SEpick @14:28:30.54 SE.seek beamBreak @14:28:32.14 SE.configure SEconfig @14:28:32.32 Elbow.reconfigure LimpElbow @14:28:32.36 Forearm.reconfigure LooseForearm @14:28:32.41 Forearm.seek 2 @14:28:32.69 Elbow.hold @14:28:32.76 Elbow.reconfigure TightElbow @14:28:32.86 SE.seek 16.09 Pucks below Top Plate @14:28:33.41 Forearm.force -20 @14:28:33.45 Forearm.seek 2 + 345 counts @14:28:33.76 Forearm.reconfigure TightForearm @14:28:33.81 Forearm.seek 2 + 700 counts @14:28:34.59 SE.seek 17.09 Pucks below Top Plate @14:28:35.26 Forearm.reconfigure LooseForearm @14:28:35.31 Forearm.seek :retract @14:28:36.22 Elbow.seek CC @14:28:37.94 Elbow.reconfigure LooseElbow @14:28:38.05 Forearm.seek CC @14:28:39.01 Hand.close @14:28:39.69 CC.seek :open @14:28:44.21 Forearm.seek :retract @14:28:45.18 Elbow.reconfigure TightElbow @14:28:45.23 Elbow.seek 2 @14:28:47.17 Elbow.reconfigure LimpElbow @14:28:47.23 Forearm.seek 2 @14:28:48.24 Elbow.hold @14:28:48.31 Elbow.reconfigure TightElbow @14:28:48.36 Hand.open @14:28:49.18 Forearm.force -20 @14:28:49.22 Forearm.seek 2 + 342 counts @14:28:49.55 Forearm.reconfigure TightForearm @14:28:49.60 Forearm.seek 2 + 700 counts @14:28:49.98 SE.seek :down @14:28:51.17 SE.coast @14:28:51.22
Forearm.reconfigure LooseForearm @14:28:51.29 Forearm.seek :retract @14:28:52.21 SC.coast @14:28:52.25 Tranferring lysate to PRV @14:28:52.26 CSV.dial :air @14:28:52.60 CS.seek 10ml @14:28:52.67 CSV.dial between air and CTV @14:28:53.01 AIV.dial :bypass @14:28:53.10 Beginning samplePassC @14:28:53.11 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:28:53.19 Hand.open @14:28:53.56 Elbow.seek FlushPuck::Garage @14:28:55.55 Elbow.reconfigure LooseElbow @14:28:55.66 Forearm.seek :garage @14:28:56.66 Hand.close @14:28:57.43 Forearm.seek :retract @14:28:58.44 Elbow.reconfigure TightElbow @14:28:58.49 Elbow.seek CC @14:29:01.44 Elbow.reconfigure LooseElbow @14:29:01.56 Forearm.reconfigure TightForearm @14:29:01.61 Forearm.seek CC @14:29:03.41 CC.seek :closed @14:29:07.69 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.07 @14:29:07.76 Hand.open @14:29:08.50 Forearm.reconfigure LooseForearm @14:29:08.55 Forearm.seek :retract @14:29:09.50 AWV.select :air @14:29:09.52 AIV.dial :bypass @14:29:09.53 PSV.dial PSR @14:29:10.97 Pulling 1.5ml of PSR.flush @14:29:10.98 PSR.select :flush @14:29:11.11 PS.seek 1.5ml @14:29:27.10 delay 5 seconds @14:29:32.12 PSV.dial :waste1 @14:29:32.74 PS.seek 0ml @14:29:48.72 PSV.dial PSR @14:29:49.21 PSR.select :air @14:29:49.35 PS.seek 2.5ml @14:30:15.51 PRV.dial CBV @14:30:15.54
CBV.dial PRV @14:30:16.44 PSV.dial :mixloop @14:30:17.54 CTV.dial :air,avoiding: :intake @14:30:17.55 PTV.dial :PRVmixing @14:30:17.63 PS.seek 0ml @14:30:43.84 PSV.dial :air @14:30:44.32 PS.seek 2.5ml @14:31:10.53 PSV.dial :mixloop @14:31:11.01 PS.seek 0ml @14:31:37.15 PSV.dial :air @14:31:37.63 PS.seek 2.5ml @14:32:03.78 PSV.dial :mixloop @14:32:04.26 PS.seek 0ml @14:32:30.48 Garaging FlushPuck in Collection Clamp @14:32:30.56 Elbow.reconfigure TightElbow @14:32:30.61 Elbow.seek CC @14:32:30.87 Elbow.reconfigure LooseElbow @14:32:30.98 Forearm.seek CC @14:32:31.99 Hand.close @14:32:32.56 CC.seek :open @14:32:36.94 Forearm.seek :retract @14:32:37.93 Elbow.reconfigure TightElbow @14:32:37.98 Elbow.seek :garage @14:32:41.02 Elbow.reconfigure LooseElbow @14:32:41.13 Forearm.reconfigure TightForearm @14:32:41.18 Forearm.seek :garage @14:32:42.33 Hand.open @14:32:43.00 Forearm.reconfigure LooseForearm @14:32:43.04 Forearm.seek :retract @14:32:44.12 Connecting PS to CS to transfer sample @14:32:44.13 CBV.dial AIV @14:32:44.14 AIV.dial :bypass @14:32:44.16 PSV.dial :mixloop @14:32:44.17 PTV.dial :PRVmixing @14:32:44.18 PRV.dial AIV @14:32:44.19 CTV.dial :air,avoiding: :intake @14:32:44.21 Loading lyfil puck from tube 3 into Collection Clamp @14:32:45.02 SC.reconfigure SCtight @14:32:45.06 SC.seek 3 @14:32:47.18 SC.reconfigure SCloose @14:32:47.42 Elbow.reconfigure TightElbow @14:32:47.47 Elbow.seek 3 @14:32:49.55 Forearm.reconfigure TightForearm @14:32:49.60 Forearm.seek 3 + 700 counts @14:32:50.64 SE.reconfigure SEpick @14:32:50.71 SE.seek beamBreak @14:32:53.60 SE.configure SEconfig @14:32:53.76 Elbow.seek 3 @14:32:54.06 Forearm.seek 3 + 300 counts @14:32:54.36 Forearm.reconfigure LooseForearm @14:32:54.40 Hand.close @14:32:55.08 SE.seek :down @14:32:55.31
Forearm.seek :retract @14:32:56.29 Elbow.seek CC @14:32:57.70 SE.coast @14:32:57.92
Elbow.reconfigure LooseElbow @14:32:58.03 Forearm.reconfigure TightForearm @14:32:58.08 Forearm.seek CC @14:32:59.37 CC.seek :closed @14:33:03.63 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.15 @14:33:03.65 Hand.open @14:33:04.38 Forearm.reconfigure LooseForearm @14:33:04.43 Forearm.seek :retract @14:33:05.41 SC.coast @14:33:05.45 CTV.dial CSV,avoiding: :intake @14:33:06.86 CSV.dial CTV @14:33:07.19 CS.seek 5ml @14:33:21.03 delay 1:20 @14:34:41.06 CS.seek 2.5ml @14:34:48.47 delay 40 seconds @14:35:28.50 CS.seek 0ml @14:35:35.81 delay 1:00 @14:36:35.83 PSV.dial :air @14:36:36.31 PS.seek 2.5ml @14:37:02.44 PSV.dial :mixloop @14:37:02.92 delay 3 seconds @14:37:05.93 PSV.dial :air @14:37:06.42 delay 2 seconds @14:37:08.43 PSV.dial :mixloop @14:37:08.92 delay 3 seconds @14:37:11.94 PSV.dial :waste1 @14:37:13.34 PS.seek :empty @14:37:39.41 CBV.dial :waste1 @14:37:39.92 delay 5 seconds @14:37:44.95 CBV.dial AIV @14:37:45.47 delay 10 seconds @14:37:55.50 CBV.dial :waste1 @14:37:56.00 delay 10 seconds @14:38:06.03 CBV.dial AIV @14:38:06.55 delay 10 seconds @14:38:16.58 CTV.dial CSV,avoiding: :intake @14:38:16.59 CBV.dial between AIV and waste1 @14:38:16.94 CSV.dial :air @14:38:18.00 CS.seek 10ml @14:38:44.55 CSV.dial CTV @14:38:45.05 CS.seek 0ml @14:39:11.59 CBV.dial :waste1 @14:39:11.94 delay 10 seconds @14:39:21.97 CTV.dial :air,avoiding: :intake @14:39:23.44 Storing lyfil puck in Collection Clamp to tube 2 @14:39:23.64 SC.reconfigure SCtight @14:39:23.68 SC.seek 2 @14:39:25.83 SC.reconfigure SCloose @14:39:26.06 Elbow.reconfigure TightElbow @14:39:26.11 Elbow.seek 2 @14:39:28.00 Forearm.reconfigure TightForearm @14:39:28.05 Forearm.seek 2 + 700 counts @14:39:29.17 SE.reconfigure SEpick @14:39:29.24 SE.seek beamBreak @14:39:30.69 SE.configure SEconfig @14:39:30.87 Elbow.reconfigure LimpElbow @14:39:30.92 Forearm.reconfigure LooseForearm @14:39:30.96 Forearm.seek 2 @14:39:31.29 Elbow.hold @14:39:31.36 Elbow.reconfigure TightElbow @14:39:31.46 SE.seek 17.09 Pucks below Top Plate @14:39:32.03 Forearm.force -20 @14:39:32.07 Forearm.seek 2 + 338 counts @14:39:32.39 Forearm.reconfigure TightForearm @14:39:32.44 Forearm.seek 2 + 700 counts @14:39:32.77 SE.seek 18.09 Pucks below Top Plate @14:39:33.44 Forearm.reconfigure LooseForearm @14:39:33.48 Forearm.seek :retract @14:39:34.44 Elbow.seek CC @14:39:36.07 Elbow.reconfigure LooseElbow @14:39:36.18 Forearm.seek CC @14:39:37.15 Hand.close @14:39:37.82 CC.seek :open @14:39:42.35 Forearm.seek :retract @14:39:43.35 Elbow.reconfigure TightElbow @14:39:43.40 Elbow.seek 2 @14:39:45.28 Elbow.reconfigure LimpElbow @14:39:45.34 Forearm.seek 2 @14:39:46.32 Elbow.hold @14:39:46.39 Elbow.reconfigure TightElbow @14:39:46.44 Hand.open @14:39:47.21 Forearm.force -20 @14:39:47.25 Forearm.seek 2 + 348 counts @14:39:47.56 Forearm.reconfigure TightForearm @14:39:47.61 Forearm.seek 2 + 700 counts @14:39:47.99 SE.seek :down @14:39:48.98 SE.coast @14:39:49.71
Forearm.reconfigure LooseForearm @14:39:49.76 Forearm.seek :retract @14:39:50.66 SC.coast @14:39:50.69 PSV.dial :air @14:39:51.86 PS.seek 0.499ml @14:39:57.78 delay 3 seconds @14:40:00.80 PSV.dial :mixloop @14:40:01.28 PRV.dial :air @14:40:01.78 delay 3 seconds @14:40:04.80 PRV.dial CBV @14:40:05.28 CBV.dial AIV @14:40:05.79 PRV.dial between CBV and air @14:40:06.17 PRV.dial :air @14:40:06.57 PS.reconfigure PSslow @14:40:06.61 PS.seek 0.599ml @14:40:09.13 PRV.dial :waste2 @14:40:09.92 PS.reconfigure PSconfig @14:40:09.97 PS.seek :empty @14:40:16.86 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:40:16.93 Hand.open @14:40:17.32 Elbow.seek FlushPuck::Garage @14:40:19.35 Elbow.reconfigure LooseElbow @14:40:19.46 Forearm.seek :garage @14:40:20.48 Hand.close @14:40:21.20 Forearm.seek :retract @14:40:22.23 Elbow.reconfigure TightElbow @14:40:22.28 Elbow.seek CC @14:40:25.25 Elbow.reconfigure LooseElbow @14:40:25.36 Forearm.reconfigure TightForearm @14:40:25.41 Forearm.seek CC @14:40:26.68 CC.seek :closed @14:40:30.93 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.08 @14:40:31.01 Hand.open @14:40:31.76 Forearm.reconfigure LooseForearm @14:40:31.80 Forearm.seek :retract @14:40:32.88 Clearing PML for modified lysate @14:40:32.89 CTV.dial :air,avoiding: :intake @14:40:32.90 PRV.dial :waste2 @14:40:32.91 PTV.dial :PRV,avoiding: :puck @14:40:33.70 delay 2 seconds @14:40:35.72 PTV.dial :PRVmixing,avoiding: :puck @14:40:36.51 delay 2 seconds @14:40:38.52 PSV.dial :PML @14:40:38.54 delay 2 seconds @14:40:40.56 PRV.dial :air @14:40:41.36 delay 2 seconds @14:40:43.44 PS.seek 1.499ml @14:40:59.42 delay 5 seconds @14:41:04.45 PS.seek 2.5ml @14:41:15.44 PSV.dial :air @14:41:15.92 PS.seek 0ml @14:41:42.05 mixing lysate with speDil @14:41:42.06 PSV.dial :PML @14:41:43.09 CBV.dial :PRV @14:41:43.10 PRV.dial :air @14:41:43.11 delay 2 seconds @14:41:45.13 PRV.dial :speDil @14:41:45.64 delay 2 seconds @14:41:47.73 PS.reconfigure PSslow1 @14:41:47.78 PS.seek 0.225ml @14:42:11.48 delay 5 seconds @14:42:16.51 PRV.dial CBV @14:42:17.33 delay 2 seconds @14:42:19.42 PS.seek 0.475ml @14:42:45.20 delay 5 seconds @14:42:50.22 PRV.dial :air @14:42:50.73 delay 2 seconds @14:42:52.76 PS.seek 0.965ml @14:43:42.73 delay 10 seconds @14:43:52.75 PRV.dial CBV @14:43:53.25 CTV.dial :air,avoiding: :intake @14:43:53.26 PSV.dial :air @14:43:53.73 PS.reconfigure PSconfig @14:43:53.78 PS.seek 2.5ml @14:44:10.14 delay 3 seconds @14:44:13.15 PSV.dial PTV @14:44:14.05 PTV.dial :PRV,avoiding: :puck @14:44:14.84 PS.seek 0ml @14:44:40.88 PSV.dial :air @14:44:41.66 PS.seek 2.5ml @14:45:07.80 delay 3 seconds @14:45:10.82 PSV.dial PTV @14:45:11.59 PTV.dial :PRV,avoiding: :puck @14:45:11.61 PS.seek 0ml @14:45:37.83 Pushing modified lysate into AIV Injection Loop @14:45:37.84 CBV.dial AIV @14:45:37.85 AIV.dial :bypass @14:45:37.86 PRV.dial CBV @14:45:37.88 PSV.dial :air @14:45:38.66 PS.seek 2.5ml @14:46:04.83 delay 3 seconds @14:46:07.84 PSV.dial :PML @14:46:08.33 PTV.dial :PRVmixing,avoiding: :puck @14:46:09.20 PS.reconfigure PSslow1 @14:46:09.25 PS.seek 1.74ml @14:47:26.86 delay 5 seconds @14:47:31.88 AIV.dial :sample @14:47:32.50 AWV.select :waste @14:47:32.68 PS.reconfigure PSslow2 @14:47:32.73 PS.seek 1.22ml @14:49:20.71 delay 15 seconds @14:49:35.73 AIV.dial :bypass @14:49:36.34 PS.reconfigure PSconfig @14:49:36.39 PS.seek 0ml @14:49:49.63 PSV.dial :air @14:49:50.11 PS.seek 2.5ml @14:50:16.29 PSV.dial :PML @14:50:16.77 PS.seek 0ml @14:50:42.92 AIV.dial :coilToMFB @14:50:42.94 AWV.select :air @14:50:43.11 Extracting Nucleic Acid from AIV @14:50:43.14
Beginning samplePassD @14:50:43.16 SPE.hold 2:46:40,55C @14:50:43.25 ASV.dial :SPEdelivery @14:50:43.34
AWV.select :air @14:50:43.35 AIV.dial :bypass @14:50:43.37 PSV.dial PSR @14:50:43.67 delay 20 seconds @14:50:44.56
Pulling 1.5ml of PSR.killMFB @14:50:44.57 PSR.select :killMFB @14:50:44.70 PS.seek 1.5ml @14:51:00.68 delay 5 seconds @14:51:03.70 primeLysateINT @14:51:03.71 AWV.select :air @14:51:03.72 AIV.dial :coilToMFB @14:51:03.73 ATV.dial AIV @14:51:05.56 AS.reconfigure ASslow1 @14:51:05.62 AS.seek 0.299ml @14:51:05.69
PSV.dial :waste1 @14:51:06.75 PS.seek 0ml @14:51:22.79 PSV.dial PSR @14:51:23.30 PSR.select :air @14:51:23.43 PS.seek 2.5ml @14:51:49.61 PRV.dial CBV @14:51:49.64
CBV.dial PRV @14:51:49.65 PSV.dial :mixloop @14:51:50.77 CTV.dial :air,avoiding: :intake @14:51:50.78 PTV.dial :PRVmixing @14:51:50.80 PS.seek 0ml @14:52:06.23 delay 10 seconds @14:52:16.25 ATV.dial :air2 @14:52:16.89
PSV.dial PSR @14:52:17.09 AS.seek 0.329ml @14:52:18.08
Pulling 1.5ml of PSR.flush @14:52:18.09 PSR.select :flush @14:52:18.22 PS.seek 1.5ml @14:52:23.40 ATV.dial :waste2 @14:52:23.97 AS.reconfigure ASnorm @14:52:24.02 AS.seek :empty @14:52:34.21
delay 5 seconds @14:52:39.23 PSV.dial :waste1 @14:52:39.75 PS.seek 0ml @14:52:41.03 Lysate primed! @14:52:41.04 AFV.select :holdingCoil @14:52:41.05 ATV.dial :air2 @14:52:41.68 AS.reconfigure ASslow1 @14:52:41.73 AS.seek 0.069ml @14:52:55.76
PSV.dial PSR @14:52:56.17 delay 3 seconds @14:52:56.25
PSR.select :air @14:52:56.39 PS.seek 2.5ml @14:52:59.18 ATV.dial :AIV @14:53:00.04 AS.seek 0.469ml @14:53:22.49 PRV.dial CBV @14:53:22.52
CBV.dial PRV @14:53:22.53 PSV.dial :mixloop @14:53:23.66 CTV.dial :air,avoiding: :intake @14:53:23.67 PTV.dial :PRVmixing @14:53:23.69 PS.seek 0ml @14:53:49.79 PSV.dial PSR @14:53:50.97 Pulling 1.5ml of PSR.flush @14:53:50.98 PSR.select :flush @14:53:51.11 PS.seek 1.5ml @14:54:07.24 delay 5 seconds @14:54:12.26 PSV.dial :waste1 @14:54:12.77 PS.seek 0ml @14:54:20.74 delay 5 seconds @14:54:25.83 Solid Phase Extraction Heater at 48.1C @14:54:25.84 ATV.dial ASV @14:54:26.23 ASV.dial :SPEdelivery @14:54:26.32 AS.seek 0.041ml @14:54:28.79
PSV.dial PSR @14:54:29.40 PSR.select :air @14:54:29.54 PS.seek 2.5ml @14:54:55.68 PRV.dial CBV @14:54:55.71
CBV.dial PRV @14:54:55.72 PSV.dial :mixloop @14:54:56.94 CTV.dial :air,avoiding: :intake @14:54:56.95 PTV.dial :PRVmixing @14:54:56.97 PS.seek 0ml @14:55:23.11 PSV.dial PSR @14:55:24.27 Pulling 1.5ml of PSR.flush @14:55:24.28 PSR.select :flush @14:55:24.41 PS.seek 1.5ml @14:55:40.49 delay 5 seconds @14:55:45.51 PSV.dial :waste1 @14:55:46.01 PS.seek 0ml @14:55:52.65 delay 10 seconds @14:56:01.98
PSV.dial PSR @14:56:02.47 PSR.select :air @14:56:02.60 PS.seek 2.5ml @14:56:02.68 rinseLysateLineINT @14:56:02.69 AFV.select :carrierSolution @14:56:02.80 delay 2 seconds @14:56:04.83 AS.reconfigure ASconfig @14:56:04.88 AS.seek 1ml @14:56:28.74 PRV.dial CBV @14:56:28.77
CBV.dial PRV @14:56:28.78 PSV.dial :mixloop @14:56:29.88 CTV.dial :air,avoiding: :intake @14:56:29.89 PTV.dial :PRVmixing @14:56:29.91 PS.reconfigure PSslow @14:56:29.96 PS.seek 1.2ml @14:56:56.77 PSV.dial :waste1 @14:56:58.18 PS.reconfigure PSconfig @14:56:58.23 PS.seek 0ml @14:57:00.85 delay 1 second @14:57:01.86 AFV.select :holdingCoil @14:57:01.98 delay 1 second @14:57:03.01 ATV.dial AIV @14:57:03.55 AIV.dial :coilToMFB @14:57:03.56 AWV.select :waste @14:57:03.67 AS.reconfigure ASslow0 @14:57:03.72 AS.seek 0ml @14:57:11.17
PSV.dial :air @14:57:12.27 PS.seek 2.5ml @14:57:38.30 PSV.dial :mixloop @14:57:38.79 PS.reconfigure PSslow @14:57:38.84 PS.seek 0ml @14:58:29.98 PSV.dial :air @14:58:30.46 PS.reconfigure PSconfig @14:58:30.51 PS.seek 2.5ml @14:58:44.59 ATV.dial :air2 @14:58:45.26 AS.reconfigure ASslow1 @14:58:45.31 AS.seek 0.05ml @14:58:55.76 ATV.dial :AIV @14:58:56.59 AS.seek 0.01ml @14:58:56.66
PSV.dial :mixloop @14:58:57.13 PS.reconfigure PSslow @14:58:57.18 PS.seek 0ml @14:59:04.91 ATV.dial :waste @14:59:06.47 AS.seek :empty @14:59:08.91 ethanol wash @14:59:08.92 ATV.dial :air2 @14:59:10.12 AS.reconfigure ASslow2 @14:59:10.18 AS.seek 0.009ml @14:59:20.67 delay 3 seconds @14:59:23.69 Pulling 0.4ml of Ethanol Wash @14:59:23.70 ATV.dial :ethanol @14:59:24.48 AS.reconfigure ASslow1 @14:59:24.54 AS.seek 0.409ml @14:59:48.37
Cleaning Collection Syringe @14:59:48.38 CSV.dial CSR @14:59:48.91 Pulling 4ml of CSR.flush @14:59:48.92 CSR.select :flush @14:59:49.05 CS.seek 4ml @15:00:00.24 delay 5 seconds @15:00:05.26 CSR.select :air @15:00:05.39 CS.seek 10ml @15:00:21.65 CSV.dial CTV @15:00:21.67
CTV.dial CSV @15:00:23.16 CBV.dial :waste1 @15:00:23.66 CS.seek 0ml @15:00:45.25 delay 3 seconds @15:00:48.27 ATV.dial :air1 @15:00:48.66 delay 3 seconds @15:00:50.17
CSV.dial CSR @15:00:50.72 Pulling 4ml of CSR.flush @15:00:50.73 CSR.select :flush @15:00:50.86 CS.seek 4ml @15:00:51.73 AS.seek 0.434ml @15:00:57.03 delay 3 seconds @15:01:00.05 ATV.dial :ethanol @15:01:00.76 AS.seek 0.419ml @15:01:02.19
delay 5 seconds @15:01:04.06 delay 3 seconds @15:01:07.08 ATV.dial ASV @15:01:07.20
CSR.select :air @15:01:07.34 CS.seek 10ml @15:01:08.35 AS.seek 0.092ml @15:01:23.71 CSV.dial CTV @15:01:23.74
CTV.dial CSV @15:01:24.22 CBV.dial :waste1 @15:01:24.81 CS.seek 0ml @15:01:51.40 Garaging FlushPuck in Collection Clamp @15:01:51.47 Elbow.reconfigure TightElbow @15:01:51.52 Elbow.seek CC @15:01:51.87 Elbow.reconfigure LooseElbow @15:01:52.01 Forearm.seek CC @15:01:53.00 Hand.close @15:01:53.60 CC.seek :open @15:01:57.96 Forearm.seek :retract @15:01:58.98 Elbow.reconfigure TightElbow @15:01:59.03 Elbow.seek :garage @15:02:02.08 Elbow.reconfigure LooseElbow @15:02:02.20 Forearm.reconfigure TightForearm @15:02:02.25 Forearm.seek :garage @15:02:03.39 Hand.open @15:02:04.05 Forearm.reconfigure LooseForearm @15:02:04.10 Forearm.seek :retract @15:02:05.13 Whole Cell Archive sampling 1500ml @15:02:05.15 Loading wcr puck from tube 3 into Collection Clamp @15:02:05.41 SC.reconfigure SCtight @15:02:05.46 SC.seek 3 @15:02:07.65 SC.reconfigure SCloose @15:02:07.88 Elbow.reconfigure TightElbow @15:02:07.93 Elbow.seek 3 @15:02:10.14 Forearm.reconfigure TightForearm @15:02:10.20 Forearm.seek 3 + 700 counts @15:02:11.28 SE.reconfigure SEpick @15:02:11.36 SE.seek beamBreak @15:02:14.24 SE.configure SEconfig @15:02:14.43 delay 3 seconds @15:02:14.47 Elbow.seek 3 @15:02:14.78 Forearm.seek 3 + 300 counts @15:02:15.05 Forearm.reconfigure LooseForearm @15:02:15.11 Hand.close @15:02:15.75 SE.seek :down @15:02:15.99 Forearm.seek :retract @15:02:16.96 Elbow.seek CC @15:02:17.47 ATV.dial :waste2 @15:02:18.21 AS.reconfigure ASconfig @15:02:18.26 AS.seek :empty @15:02:18.39 SE.coast @15:02:18.69 Elbow.reconfigure LooseElbow @15:02:18.80 Forearm.reconfigure TightForearm @15:02:18.86 Forearm.seek CC @15:02:20.79 CC.seek :closed @15:02:23.90 ATV.dial :air2 @15:02:24.30 AS.reconfigure ASslow1 @15:02:24.38 AS.seek 1ml @15:02:25.09 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.10 @15:02:25.10 Hand.open @15:02:25.80 Forearm.reconfigure LooseForearm @15:02:25.86 Forearm.seek :retract @15:02:26.84 SC.coast @15:02:26.88 collectSample 1500ml w/bubble point of 55psi @15:02:26.90 Priming 7ml @15:02:26.91 Intake.select :close @15:02:26.92 Exhaust.select :close @15:02:26.93 SS.hold @15:02:27.17 Calibrating pressure sensors @15:02:27.18 CTV.dial :air @15:02:28.60 SSV.dial :spit,avoiding: :exhaust @15:02:28.62 SS.reconfigure SSfast @15:02:28.67 SS.seek 5ml @15:02:31.35 SSV.dial CBV @15:02:31.38 CBV.dial SSV @15:02:32.91 delay 3.8 seconds @15:02:36.86 SSV.dial :spit @15:02:38.29 SS.seek 0ml @15:02:41.17 SSV.dial CBV @15:02:42.61 CTV.dial :intake @15:02:43.41 SSV.dial CBV @15:02:43.44 CBV.dial SSV @15:02:43.45 Allowing 40s for 7.0ml of remaining 7.0ml @15:02:43.47 Intake.select :open @15:02:45.65 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:02:45.67 SS.reconfigure SSinhale.with maxSpeed: 150 @15:02:45.71 SS.seek 7ml @15:02:48.89 Vacuum too high -- waiting up to 40s @15:02:51.26 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:02:51.28 SS.reconfigure SSinhale.with maxSpeed: 135 @15:02:51.33 SS.seek 7ml @15:02:54.63 Stroke completed -- waiting for pressure to equalize ... @15:02:59.13 Intake.select :close @15:02:59.28 SSV.dial :exhaust @15:03:00.13 Exhaust.select :open @15:03:00.24 SS.reconfigure SSexhale @15:03:00.29 SS.seek 0ml @15:03:04.15 Exhaust.select :close @15:03:04.27 Sampling 1500.0ml @15:03:04.28 Intake.select :close @15:03:04.30 Exhaust.select :close @15:03:04.31 SS.reconfigure SSconfig @15:03:04.36 SS.hold @15:03:05.18 CTV.dial :intake @15:03:05.20 SSV.dial CBV @15:03:05.23 CBV.dial SSV @15:03:06.07 Allowing 130s for 25.0ml of remaining 1500.0ml @15:03:06.08 Intake.select :open @15:03:08.27 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:03:08.29 SS.reconfigure SSinhale.with maxSpeed: 135 @15:03:08.34 SS.seek 25ml @15:03:11.83 Vacuum too high -- waiting up to 40s @15:03:13.45 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:03:13.47 SS.reconfigure SSinhale.with maxSpeed: 122 @15:03:13.52 SS.seek 25ml @15:03:16.17 Vacuum too high -- waiting up to 40s @15:03:17.79 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:03:17.81 SS.reconfigure SSinhale.with maxSpeed: 110 @15:03:17.86 SS.seek 25ml @15:03:20.92 Vacuum too high -- waiting up to 40s @15:03:22.53 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:03:22.55 SS.reconfigure SSinhale.with maxSpeed: 99 @15:03:22.59 SS.seek 25ml @15:03:25.30 Vacuum too high -- waiting up to 40s @15:03:26.66 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:03:26.68 SS.reconfigure SSinhale.with maxSpeed: 89 @15:03:26.73 SS.seek 25ml @15:03:29.73 Vacuum too high -- waiting up to 40s @15:03:31.10 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:03:31.12 SS.reconfigure SSinhale.with maxSpeed: 80 @15:03:31.17 SS.seek 25ml @15:03:34.42 Vacuum too high -- waiting up to 40s @15:03:35.80 SS.reconfigure SSinhale.with maxSpeed: 72 @15:03:35.86 SS.seek 25ml @15:03:39.48 Vacuum too high -- waiting up to 40s @15:03:40.67 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:03:40.69 SS.reconfigure SSinhale.with maxSpeed: 65 @15:03:40.74 SS.seek 25ml @15:03:44.96 Vacuum too high -- waiting up to 40s @15:03:46.05 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:03:46.07 SS.reconfigure SSinhale.with maxSpeed: 59 @15:03:46.12 SS.seek 25ml @15:03:47.01 Stroke completed -- waiting for pressure to equalize ... @15:03:48.98 Intake.select :close @15:03:49.15 SSV.dial :exhaust @15:03:49.97 Exhaust.select :open @15:03:50.08 SS.reconfigure SSexhale @15:03:50.14 SS.seek 0ml @15:03:59.13 Exhaust.select :close @15:03:59.25 SSV.dial CBV @15:03:59.28 CBV.dial SSV @15:04:00.13 Allowing 130s for 25.0ml of remaining 1475.0ml @15:04:00.14 Intake.select :open @15:04:02.32 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:04:02.34 SS.reconfigure SSinhale.with maxSpeed: 59 @15:04:02.40 SS.seek 25ml @15:04:10.30 Vacuum too high -- waiting up to 40s @15:04:11.40 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:04:11.42 SS.reconfigure SSinhale.with maxSpeed: 53 @15:04:11.47 SS.seek 25ml @15:04:35.42 Vacuum too high -- waiting up to 40s @15:04:36.52 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:04:36.54 SS.reconfigure SSinhale.with maxSpeed: 48 @15:04:36.59 SS.seek 25ml @15:04:38.68 Stroke completed -- waiting for pressure to equalize ... @15:04:41.30 Intake.select :close @15:04:41.47 SSV.dial :exhaust @15:04:42.31 Exhaust.select :open @15:04:42.43 SS.reconfigure SSexhale @15:04:42.48 SS.seek 0ml @15:04:51.56 Exhaust.select :close @15:04:51.68 SSV.dial CBV @15:04:51.71 CBV.dial SSV @15:04:52.55 Allowing 130s for 25.0ml of remaining 1450.0ml @15:04:52.56 Intake.select :open @15:04:54.74 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:04:54.76 SS.reconfigure SSinhale.with maxSpeed: 48 @15:04:54.81 SS.seek 25ml @15:05:31.98 Stroke completed -- waiting for pressure to equalize ... @15:05:35.21 Intake.select :close @15:05:35.38 SSV.dial :exhaust @15:05:36.78 Exhaust.select :open @15:05:36.89 SS.reconfigure SSexhale @15:05:36.94 SS.seek 0ml @15:05:45.96 delay 5 seconds @15:05:46.15 Exhaust.select :close @15:05:46.27 SSV.dial CBV @15:05:46.30 CBV.dial SSV @15:05:47.16 Allowing 130s for 25.0ml of remaining 1425.0ml @15:05:47.17 Intake.select :open @15:05:49.35 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:05:49.37 SS.reconfigure SSinhale.with maxSpeed: 48 @15:05:49.42 SS.seek 25ml @15:05:50.97 ATV.dial ASV @15:05:51.94 ASV.dial :SPEdelivery @15:05:51.96 AS.seek 0.02ml @15:06:26.35 Stroke completed -- waiting for pressure to equalize ... @15:06:29.58 Intake.select :close @15:06:29.75 SSV.dial :exhaust @15:06:30.58 Exhaust.select :open @15:06:30.69 SS.reconfigure SSexhale @15:06:30.75 SS.seek 0ml @15:06:39.98 Exhaust.select :close @15:06:40.10 SSV.dial CBV @15:06:40.13 CBV.dial SSV @15:06:40.99 Allowing 130s for 25.0ml of remaining 1400.0ml @15:06:41.00 Intake.select :open @15:06:43.18 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:06:43.22 SS.reconfigure SSinhale.with maxSpeed: 48 @15:06:43.32 SS.seek 25ml @15:07:20.29 Stroke completed -- waiting for pressure to equalize ... @15:07:23.52 Intake.select :close @15:07:23.68 SSV.dial :exhaust @15:07:24.52 Exhaust.select :open @15:07:24.63 SS.reconfigure SSexhale @15:07:24.68 SS.seek 0ml @15:07:33.85 Exhaust.select :close @15:07:33.97 SSV.dial CBV @15:07:34.00 CBV.dial SSV @15:07:34.83 Allowing 130s for 25.0ml of remaining 1375.0ml @15:07:34.84 Intake.select :open @15:07:37.02 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:07:37.04 SS.reconfigure SSinhale.with maxSpeed: 48 @15:07:37.09 SS.seek 25ml @15:08:14.26 Stroke completed -- waiting for pressure to equalize ... @15:08:17.49 Intake.select :close @15:08:17.64 SSV.dial :exhaust @15:08:18.46 Exhaust.select :open @15:08:18.58 SS.reconfigure SSexhale @15:08:18.63 SS.seek 0ml @15:08:27.40 Exhaust.select :close @15:08:27.52 SSV.dial CBV @15:08:27.55 CBV.dial SSV @15:08:28.39 Allowing 130s for 25.0ml of remaining 1350.0ml @15:08:28.40 Intake.select :open @15:08:30.58 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:08:30.60 SS.reconfigure SSinhale.with maxSpeed: 48 @15:08:30.65 SS.seek 25ml @15:09:08.37 Stroke completed -- waiting for pressure to equalize ... @15:09:09.29 delay 5 seconds @15:09:12.26 Intake.select :close @15:09:12.42 SSV.dial :exhaust @15:09:13.24 Exhaust.select :open @15:09:13.35 SS.reconfigure SSexhale @15:09:13.42 SS.seek 0ml @15:09:14.31 ATV.dial :air2 @15:09:15.33 AS.seek 0.52ml @15:09:22.49 Exhaust.select :close @15:09:22.61 SSV.dial CBV @15:09:22.64 CBV.dial SSV @15:09:23.49 Allowing 130s for 25.0ml of remaining 1325.0ml @15:09:23.50 Intake.select :open @15:09:25.69 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:09:25.71 SS.reconfigure SSinhale.with maxSpeed: 48 @15:09:25.76 SS.seek 25ml @15:09:51.76 Vacuum too high -- waiting up to 40s @15:09:52.88 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:09:52.90 SS.reconfigure SSinhale.with maxSpeed: 43 @15:09:52.95 SS.seek 25ml @15:10:05.54 Stroke completed -- waiting for pressure to equalize ... @15:10:08.79 Intake.select :close @15:10:08.95 SSV.dial :exhaust @15:10:09.76 Exhaust.select :open @15:10:09.88 SS.reconfigure SSexhale @15:10:09.93 SS.seek 0ml @15:10:18.74 Exhaust.select :close @15:10:18.86 SSV.dial CBV @15:10:18.89 CBV.dial SSV @15:10:19.74 Allowing 130s for 25.0ml of remaining 1300.0ml @15:10:19.75 Intake.select :open @15:10:21.93 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:10:21.95 SS.reconfigure SSinhale.with maxSpeed: 43 @15:10:22.00 SS.seek 25ml @15:10:56.19 delay 5 seconds @15:11:01.21 ATV.dial ASV @15:11:02.19 ASV.dial :wasteR @15:11:02.55 AS.seek 0.3ml @15:11:03.68 Stroke completed -- waiting for pressure to equalize ... @15:11:06.98 Intake.select :close @15:11:07.15 SSV.dial :exhaust @15:11:07.97 Exhaust.select :open @15:11:08.08 SS.reconfigure SSexhale @15:11:08.13 SS.seek 0ml @15:11:17.62 Exhaust.select :close @15:11:17.74 SSV.dial CBV @15:11:17.77 CBV.dial SSV @15:11:18.63 Allowing 130s for 25.0ml of remaining 1275.0ml @15:11:18.64 Intake.select :open @15:11:20.82 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:11:20.84 SS.reconfigure SSinhale.with maxSpeed: 43 @15:11:20.89 SS.seek 25ml @15:11:47.08 delay 20 seconds @15:12:02.37 Stroke completed -- waiting for pressure to equalize ... @15:12:05.53 Intake.select :close @15:12:05.69 SSV.dial :exhaust @15:12:06.53 Exhaust.select :open @15:12:06.64 SS.reconfigure SSexhale @15:12:06.70 SS.seek 0ml @15:12:07.10 AS.seek 0.07ml @15:12:15.74 Exhaust.select :close @15:12:15.86 SSV.dial CBV @15:12:15.89 CBV.dial SSV @15:12:16.74 Allowing 130s for 25.0ml of remaining 1250.0ml @15:12:16.75 Intake.select :open @15:12:18.94 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:12:18.96 SS.reconfigure SSinhale.with maxSpeed: 43 @15:12:19.01 SS.seek 25ml @15:12:53.65 delay 20 seconds @15:13:00.51 Stroke completed -- waiting for pressure to equalize ... @15:13:03.75 Intake.select :close @15:13:03.92 SSV.dial :exhaust @15:13:04.75 Exhaust.select :open @15:13:04.86 SS.reconfigure SSexhale @15:13:04.92 SS.seek 0ml @15:13:13.67 ATV.dial :waste2 @15:13:13.76 Exhaust.select :close @15:13:13.88 SSV.dial CBV @15:13:13.91 CBV.dial SSV @15:13:14.41 AS.reconfigure ASconfig @15:13:14.46 AS.seek :empty @15:13:14.75 Allowing 130s for 25.0ml of remaining 1225.0ml @15:13:14.76 Intake.select :open @15:13:16.95 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:13:16.97 SS.reconfigure SSinhale.with maxSpeed: 43 @15:13:17.01 SS.seek 25ml @15:13:18.85 Elution @15:13:18.87 ATV.dial :air1 @15:13:19.97 AS.reconfigure ASslow1 @15:13:20.02 AS.seek 0.049ml @15:13:30.37 delay 3 seconds @15:13:33.39 ATV.dial :ElutionWater @15:13:34.08 AS.seek 0.109ml @15:13:46.41 delay 3 seconds @15:13:49.44 ATV.dial ASV,via: :air2 @15:13:51.43 ASV.dial :SPEelution @15:13:52.00 AFV.select :carrierSolution @15:13:52.17 AS.reconfigure ASnorm @15:13:52.22 AS.seek 0.759ml @15:13:58.81 Stroke completed -- waiting for pressure to equalize ... @15:14:02.67 Intake.select :close @15:14:02.83 SSV.dial :exhaust @15:14:03.67 Exhaust.select :open @15:14:03.79 SS.reconfigure SSexhale @15:14:03.85 SS.seek 0ml @15:14:12.95 Exhaust.select :close @15:14:13.07 SSV.dial CBV @15:14:13.10 CBV.dial SSV @15:14:13.94 Allowing 130s for 25.0ml of remaining 1200.0ml @15:14:13.95 Intake.select :open @15:14:16.14 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:14:16.16 SS.reconfigure SSinhale.with maxSpeed: 43 @15:14:16.20 SS.seek 25ml @15:14:25.26 delay 3 seconds @15:14:28.28 AFV.select :holdingCoil @15:14:28.44 AS.reconfigure ASslow1 @15:14:28.49 AS.seek 0.474ml @15:14:57.40 Stroke completed -- waiting for pressure to equalize ... @15:15:01.28 Intake.select :close @15:15:01.45 SSV.dial :exhaust @15:15:02.27 Exhaust.select :open @15:15:02.38 SS.reconfigure SSexhale @15:15:02.45 SS.seek 0ml @15:15:11.35 Exhaust.select :close @15:15:11.47 SSV.dial CBV @15:15:11.50 CBV.dial SSV @15:15:12.35 Allowing 130s for 25.0ml of remaining 1175.0ml @15:15:12.36 Intake.select :open @15:15:14.55 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:15:14.57 SS.reconfigure SSinhale.with maxSpeed: 43 @15:15:14.62 SS.seek 25ml @15:15:26.09 delay 5 seconds @15:15:31.18 AS.reconfigure ASslow2 @15:15:31.23 AS.seek 0.439ml @15:15:56.04 Stroke completed -- waiting for pressure to equalize ... @15:16:00.57 Intake.select :close @15:16:00.73 SSV.dial :exhaust @15:16:01.56 Exhaust.select :open @15:16:01.67 SS.reconfigure SSexhale @15:16:01.72 SS.seek 0ml @15:16:06.62 delay 1:00 @15:16:10.57 Exhaust.select :close @15:16:10.69 SSV.dial CBV @15:16:10.72 CBV.dial SSV @15:16:11.57 Allowing 130s for 25.0ml of remaining 1150.0ml @15:16:11.58 Intake.select :open @15:16:13.78 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:16:13.80 SS.reconfigure SSinhale.with maxSpeed: 43 @15:16:13.85 SS.seek 25ml @15:16:54.33 Vacuum too high -- waiting up to 40s @15:16:55.70 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:16:55.72 SS.reconfigure SSinhale.with maxSpeed: 39 @15:16:55.77 SS.seek 25ml @15:16:57.31 Stroke completed -- waiting for pressure to equalize ... @15:17:00.55 Intake.select :close @15:17:00.72 SSV.dial :exhaust @15:17:01.57 Exhaust.select :open @15:17:01.68 SS.reconfigure SSexhale @15:17:01.74 SS.seek 0ml @15:17:06.65 pushing eluate to holding coil @15:17:06.72 AS.seek 0.279ml @15:17:11.24 Exhaust.select :close @15:17:11.36 SSV.dial CBV @15:17:11.39 CBV.dial SSV @15:17:12.22 Allowing 130s for 25.0ml of remaining 1125.0ml @15:17:12.23 Intake.select :open @15:17:14.41 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:17:14.43 SS.reconfigure SSinhale.with maxSpeed: 39 @15:17:14.48 SS.seek 25ml @15:17:59.77 Stroke completed -- waiting for pressure to equalize ... @15:18:03.60 Intake.select :close @15:18:03.76 SSV.dial :exhaust @15:18:04.58 Exhaust.select :open @15:18:04.69 SS.reconfigure SSexhale @15:18:04.74 SS.seek 0ml @15:18:13.65 Exhaust.select :close @15:18:13.77 SSV.dial CBV @15:18:13.80 CBV.dial SSV @15:18:14.66 Allowing 130s for 25.0ml of remaining 1100.0ml @15:18:14.67 Intake.select :open @15:18:16.85 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:18:16.87 SS.reconfigure SSinhale.with maxSpeed: 39 @15:18:16.92 SS.seek 25ml @15:19:02.71 Stroke completed -- waiting for pressure to equalize ... @15:19:06.59 Intake.select :close @15:19:06.75 SSV.dial :exhaust @15:19:07.57 Exhaust.select :open @15:19:07.69 SS.reconfigure SSexhale @15:19:07.76 SS.seek 0ml @15:19:16.57 Exhaust.select :close @15:19:16.69 SSV.dial CBV @15:19:16.72 CBV.dial SSV @15:19:17.57 Allowing 130s for 25.0ml of remaining 1075.0ml @15:19:17.58 Intake.select :open @15:19:19.76 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:19:19.78 SS.reconfigure SSinhale.with maxSpeed: 39 @15:19:19.83 SS.seek 25ml @15:19:47.73 delay 30 seconds @15:20:05.57 Stroke completed -- waiting for pressure to equalize ... @15:20:10.07 Intake.select :close @15:20:10.23 SSV.dial :exhaust @15:20:11.06 Exhaust.select :open @15:20:11.18 SS.reconfigure SSexhale @15:20:11.23 SS.seek 0ml @15:20:17.76 ATV.dial :waste,via: :air3 @15:20:19.38 AS.reconfigure ASconfig @15:20:19.43 AS.seek :empty @15:20:20.42 Exhaust.select :close @15:20:20.54 SSV.dial CBV @15:20:20.57 CBV.dial SSV @15:20:21.43 Allowing 130s for 25.0ml of remaining 1050.0ml @15:20:21.44 Intake.select :open @15:20:23.62 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:20:23.64 SS.reconfigure SSinhale.with maxSpeed: 39 @15:20:23.69 SS.seek 25ml @15:20:35.84 Parking Bleach in LysateSource line and SPEcolumn @15:20:35.85 ATV.dial :air3 @15:20:36.66 AS.reconfigure ASslow1 @15:20:36.72 AS.seek 0.029ml @15:20:43.06 ATV.dial :ElutionWater @15:20:43.66 AS.seek 0.059ml @15:20:49.94 ATV.dial :air3 @15:20:50.55 AS.seek 0.069ml @15:20:52.82 delay 2 seconds @15:20:54.83 ATV.dial :bleach @15:20:55.88 AS.seek 0.769ml @15:21:09.20 Stroke completed -- waiting for pressure to equalize ... @15:21:13.72 Intake.select :close @15:21:13.88 SSV.dial :exhaust @15:21:14.73 Exhaust.select :open @15:21:14.84 SS.reconfigure SSexhale @15:21:14.89 SS.seek 0ml @15:21:23.62 Exhaust.select :close @15:21:23.74 SSV.dial CBV @15:21:23.77 CBV.dial SSV @15:21:24.63 Allowing 130s for 25.0ml of remaining 1025.0ml @15:21:24.64 Intake.select :open @15:21:26.82 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:21:26.84 SS.reconfigure SSinhale.with maxSpeed: 39 @15:21:26.89 SS.seek 25ml @15:22:12.74 Stroke completed -- waiting for pressure to equalize ... @15:22:17.89 Intake.select :close @15:22:18.05 SSV.dial :exhaust @15:22:18.88 Exhaust.select :open @15:22:19.00 SS.reconfigure SSexhale @15:22:19.05 SS.seek 0ml @15:22:28.13 Exhaust.select :close @15:22:28.25 SSV.dial CBV @15:22:28.28 CBV.dial SSV @15:22:29.11 Allowing 130s for 25.0ml of remaining 1000.0ml @15:22:29.12 Intake.select :open @15:22:31.30 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:22:31.32 SS.reconfigure SSinhale.with maxSpeed: 39 @15:22:31.37 SS.seek 25ml @15:23:06.05 Vacuum too high -- waiting up to 40s @15:23:07.41 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:23:07.43 SS.reconfigure SSinhale.with maxSpeed: 35 @15:23:07.48 SS.seek 25ml @15:23:16.90 delay 3 seconds @15:23:19.70 Stroke completed -- waiting for pressure to equalize ... @15:23:19.91 ATV.dial :air3 @15:23:20.45 delay 3 seconds @15:23:23.59 AS.seek 0.794ml @15:23:24.20 Intake.select :close @15:23:24.36 SSV.dial :exhaust @15:23:25.19 Exhaust.select :open @15:23:25.30 SS.reconfigure SSexhale @15:23:25.95 SS.seek 0ml @15:23:28.90 delay 3 seconds @15:23:31.92 ATV.dial :bleach @15:23:32.53 AS.seek 0.779ml @15:23:35.20 Exhaust.select :close @15:23:35.32 SSV.dial CBV @15:23:35.35 CBV.dial SSV @15:23:35.79 delay 3 seconds @15:23:36.21 Allowing 130s for 25.0ml of remaining 975.0ml @15:23:36.22 Intake.select :open @15:23:38.40 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:23:38.43 SS.reconfigure SSinhale.with maxSpeed: 35 @15:23:38.48 SS.seek 25ml @15:23:38.80 ATV.dial :AIV @15:23:40.03 AS.reconfigure ASconfig @15:23:40.08 AS.seek 0.015ml @15:24:24.27 ATV.dial :waste @15:24:25.71 AS.seek :empty @15:24:26.91 ATV.dial :air3 @15:24:27.58 AS.reconfigure ASslow1 @15:24:27.63 AS.seek 0.009ml @15:24:28.98 Stroke completed -- waiting for pressure to equalize ... @15:24:30.05 delay 2 seconds @15:24:32.15 ATV.dial :bleach @15:24:32.72 AS.seek 0.579ml @15:24:33.59 Intake.select :close @15:24:33.76 SSV.dial :exhaust @15:24:34.59 Exhaust.select :open @15:24:34.70 SS.reconfigure SSexhale @15:24:34.76 SS.seek 0ml @15:24:44.09 Exhaust.select :close @15:24:44.21 SSV.dial CBV @15:24:44.24 CBV.dial SSV @15:24:45.10 Allowing 130s for 25.0ml of remaining 950.0ml @15:24:45.11 Intake.select :open @15:24:47.29 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:24:47.31 SS.reconfigure SSinhale.with maxSpeed: 35 @15:24:47.36 SS.seek 25ml @15:25:37.92 Stroke completed -- waiting for pressure to equalize ... @15:25:42.42 Intake.select :close @15:25:42.59 SSV.dial :exhaust @15:25:43.43 Exhaust.select :open @15:25:43.54 SS.reconfigure SSexhale @15:25:43.59 SS.seek 0ml @15:25:52.98 Exhaust.select :close @15:25:53.10 SSV.dial CBV @15:25:53.13 CBV.dial SSV @15:25:53.99 Allowing 130s for 25.0ml of remaining 925.0ml @15:25:54.00 Intake.select :open @15:25:56.18 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:25:56.20 SS.reconfigure SSinhale.with maxSpeed: 35 @15:25:56.25 SS.seek 25ml @15:26:27.64 delay 3 seconds @15:26:30.66 ATV.dial :air3 @15:26:31.20 delay 3 seconds @15:26:34.29 AS.seek 0.604ml @15:26:39.63 delay 3 seconds @15:26:42.65 ATV.dial :bleach @15:26:43.27 AS.seek 0.589ml @15:26:46.53 delay 3 seconds @15:26:46.59 Stroke completed -- waiting for pressure to equalize ... @15:26:49.54 ATV.dial :ASV @15:26:50.53 ASV.dial :wasteR @15:26:51.18 AS.seek 0ml @15:26:51.69 Intake.select :close @15:26:51.86 SSV.dial :exhaust @15:26:52.71 Exhaust.select :open @15:26:52.82 SS.reconfigure SSexhale @15:26:52.87 SS.seek 0ml @15:27:02.12 Exhaust.select :close @15:27:02.24 SSV.dial CBV @15:27:02.27 CBV.dial SSV @15:27:03.11 Allowing 130s for 25.0ml of remaining 900.0ml @15:27:03.12 Intake.select :open @15:27:05.31 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:27:05.33 SS.reconfigure SSinhale.with maxSpeed: 35 @15:27:05.38 SS.seek 25ml @15:27:55.90 Stroke completed -- waiting for pressure to equalize ... @15:28:01.07 Intake.select :close @15:28:01.23 SSV.dial :exhaust @15:28:02.07 Exhaust.select :open @15:28:02.18 SS.reconfigure SSexhale @15:28:02.23 SS.seek 0ml @15:28:11.17 Exhaust.select :close @15:28:11.29 SSV.dial CBV @15:28:11.32 CBV.dial SSV @15:28:12.18 Allowing 130s for 25.0ml of remaining 875.0ml @15:28:12.19 Intake.select :open @15:28:14.37 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:28:14.39 SS.reconfigure SSinhale.with maxSpeed: 35 @15:28:14.44 SS.seek 25ml @15:28:49.96 delay 1:00 @15:28:54.14 Vacuum too high -- waiting up to 40s @15:28:55.50 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:28:55.52 SS.reconfigure SSinhale.with maxSpeed: 32 @15:28:55.57 SS.seek 25ml @15:29:07.90 Stroke completed -- waiting for pressure to equalize ... @15:29:12.40 Intake.select :close @15:29:12.56 SSV.dial :exhaust @15:29:13.38 Exhaust.select :open @15:29:13.49 SS.reconfigure SSexhale @15:29:13.54 SS.seek 0ml @15:29:22.71 Exhaust.select :close @15:29:22.83 SSV.dial CBV @15:29:23.43 CBV.dial SSV @15:29:24.32 Allowing 130s for 25.0ml of remaining 850.0ml @15:29:24.33 Intake.select :open @15:29:26.52 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:29:26.54 SS.reconfigure SSinhale.with maxSpeed: 32 @15:29:26.59 SS.seek 25ml @15:29:49.97 AFV.select :carrierSolution @15:29:50.08 delay 2 seconds @15:29:52.11 AS.reconfigure ASconfig @15:29:52.16 AS.seek 1ml @15:30:21.74 Stroke completed -- waiting for pressure to equalize ... @15:30:26.25 Intake.select :close @15:30:26.42 SSV.dial :exhaust @15:30:27.25 Exhaust.select :open @15:30:27.37 SS.reconfigure SSexhale @15:30:27.42 SS.seek 0ml @15:30:36.18 Exhaust.select :close @15:30:36.30 SSV.dial CBV @15:30:36.33 CBV.dial SSV @15:30:37.19 Allowing 130s for 25.0ml of remaining 825.0ml @15:30:37.20 Intake.select :open @15:30:39.38 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:30:39.40 SS.reconfigure SSinhale.with maxSpeed: 32 @15:30:39.45 SS.seek 25ml @15:30:49.92 delay 1 second @15:30:50.93 AFV.select :holdingCoil @15:30:51.04 delay 1 second @15:30:52.07 ATV.dial ASV @15:30:52.08 ASV.dial :wasteR @15:30:52.10 AS.reconfigure ASslow1 @15:30:52.14 AS.seek 0ml @15:31:34.88 Stroke completed -- waiting for pressure to equalize ... @15:31:40.00 Intake.select :close @15:31:40.16 SSV.dial :exhaust @15:31:41.00 Exhaust.select :open @15:31:41.11 SS.reconfigure SSexhale @15:31:41.16 SS.seek 0ml @15:31:50.46 Exhaust.select :close @15:31:50.58 SSV.dial CBV @15:31:50.61 CBV.dial SSV @15:31:51.47 Allowing 130s for 25.0ml of remaining 800.0ml @15:31:51.48 Intake.select :open @15:31:53.66 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:31:53.68 SS.reconfigure SSinhale.with maxSpeed: 32 @15:31:53.73 SS.seek 25ml @15:32:47.05 Vacuum too high -- waiting up to 40s @15:32:48.41 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:32:48.43 SS.reconfigure SSinhale.with maxSpeed: 29 @15:32:48.47 SS.seek 25ml @15:32:50.24 Stroke completed -- waiting for pressure to equalize ... @15:32:54.72 Intake.select :close @15:32:54.88 SSV.dial :exhaust @15:32:55.71 Exhaust.select :open @15:32:55.82 SS.reconfigure SSexhale @15:32:55.88 SS.seek 0ml @15:33:05.01 Exhaust.select :close @15:33:05.13 SSV.dial CBV @15:33:05.16 CBV.dial SSV @15:33:06.02 Allowing 130s for 25.0ml of remaining 775.0ml @15:33:06.03 Intake.select :open @15:33:08.22 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:33:08.24 SS.reconfigure SSinhale.with maxSpeed: 29 @15:33:08.28 SS.seek 25ml @15:34:09.24 Stroke completed -- waiting for pressure to equalize ... @15:34:13.47 delay 20 seconds @15:34:14.42 Intake.select :close @15:34:14.58 SSV.dial :exhaust @15:34:15.42 Exhaust.select :open @15:34:15.54 SS.reconfigure SSexhale @15:34:15.59 SS.seek 0ml @15:34:24.40 Exhaust.select :close @15:34:24.52 SSV.dial CBV @15:34:24.55 CBV.dial SSV @15:34:25.41 Allowing 130s for 25.0ml of remaining 750.0ml @15:34:25.42 Intake.select :open @15:34:27.61 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:34:27.63 SS.reconfigure SSinhale.with maxSpeed: 29 @15:34:27.67 SS.seek 25ml @15:34:33.49 ATV.dial :air3 @15:34:34.96 AS.seek 0.59ml @15:35:28.68 Stroke completed -- waiting for pressure to equalize ... @15:35:34.44 Intake.select :close @15:35:34.60 SSV.dial :exhaust @15:35:35.46 Exhaust.select :open @15:35:35.57 SS.reconfigure SSexhale @15:35:35.62 SS.seek 0ml @15:35:44.87 Exhaust.select :close @15:35:44.99 SSV.dial CBV @15:35:45.02 CBV.dial SSV @15:35:45.88 Allowing 130s for 25.0ml of remaining 725.0ml @15:35:45.89 Intake.select :open @15:35:48.07 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:35:48.09 SS.reconfigure SSinhale.with maxSpeed: 29 @15:35:48.14 SS.seek 25ml @15:36:33.94 delay 3 seconds @15:36:36.96 ATV.dial :ASV @15:36:38.43 ASV.dial :wasteR @15:36:38.52 AS.seek 0.09ml @15:36:48.79 Stroke completed -- waiting for pressure to equalize ... @15:36:54.55 Intake.select :close @15:36:54.72 SSV.dial :exhaust @15:36:55.57 Exhaust.select :open @15:36:55.68 SS.reconfigure SSexhale @15:36:55.74 SS.seek 0ml @15:37:04.84 Exhaust.select :close @15:37:04.96 SSV.dial CBV @15:37:04.99 CBV.dial SSV @15:37:05.83 Allowing 130s for 25.0ml of remaining 700.0ml @15:37:05.84 Intake.select :open @15:37:08.03 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:37:08.05 SS.reconfigure SSinhale.with maxSpeed: 29 @15:37:08.10 SS.seek 25ml @15:38:08.70 Stroke completed -- waiting for pressure to equalize ... @15:38:15.13 Intake.select :close @15:38:15.29 SSV.dial :exhaust @15:38:16.10 Exhaust.select :open @15:38:16.22 SS.reconfigure SSexhale @15:38:16.28 SS.seek 0ml @15:38:19.36 delay 30 seconds @15:38:25.49 Exhaust.select :close @15:38:25.61 SSV.dial CBV @15:38:25.64 CBV.dial SSV @15:38:26.49 Allowing 130s for 25.0ml of remaining 675.0ml @15:38:26.50 Intake.select :open @15:38:28.68 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:38:28.70 SS.reconfigure SSinhale.with maxSpeed: 29 @15:38:28.75 SS.seek 25ml @15:38:49.38 ATV.dial :extSrc @15:38:50.22 delay 3 seconds @15:38:53.24 ATV.dial :ElutionWater @15:38:54.33 AS.seek 0.12ml @15:39:00.69 ATV.dial :air3 @15:39:01.30 AS.seek 0.13ml @15:39:03.62 delay 2 seconds @15:39:05.63 ATV.dial :bleach @15:39:06.10 AS.seek 0.23ml @15:39:15.33 Vacuum too high -- waiting up to 40s @15:39:16.95 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:39:16.97 SS.reconfigure SSinhale.with maxSpeed: 26 @15:39:17.02 SS.seek 25ml @15:39:26.48 delay 3 seconds @15:39:29.50 ATV.dial :air3 @15:39:30.05 delay 3 seconds @15:39:32.60 Stroke completed -- waiting for pressure to equalize ... @15:39:33.13 AS.seek 0.255ml @15:39:37.76 Intake.select :close @15:39:37.92 SSV.dial :exhaust @15:39:38.44 delay 3 seconds @15:39:38.77 Exhaust.select :open @15:39:38.88 SS.reconfigure SSexhale @15:39:38.94 SS.seek 0ml @15:39:41.46 ATV.dial :bleach @15:39:42.08 AS.seek 0.24ml @15:39:45.42 delay 3 seconds @15:39:48.13 Exhaust.select :close @15:39:48.25 SSV.dial CBV @15:39:48.28 CBV.dial SSV @15:39:48.43 ATV.dial :ASV @15:39:49.13 Allowing 130s for 25.0ml of remaining 650.0ml @15:39:49.14 Intake.select :open @15:39:49.44 ASV.dial :SPEdelivery @15:39:49.80 AFV.select :carrierSolution @15:39:49.92 delay 2 seconds @15:39:51.33 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:39:51.35 SS.reconfigure SSinhale.with maxSpeed: 26 @15:39:51.40 SS.seek 25ml @15:39:51.94 AS.reconfigure ASconfig @15:39:51.99 AS.seek 0.5ml @15:40:07.23 delay 1 second @15:40:08.24 AFV.select :holdingCoil @15:40:08.40 AS.reconfigure ASslow1 @15:40:08.45 AS.seek 0.17ml @15:40:58.67 Stroke completed -- waiting for pressure to equalize ... @15:41:04.47 Intake.select :close @15:41:04.63 SSV.dial :exhaust @15:41:05.46 Exhaust.select :open @15:41:05.58 SS.reconfigure SSexhale @15:41:05.63 SS.seek 0ml @15:41:15.02 Exhaust.select :close @15:41:15.12 SPE.hold 2:46:40,95C @15:41:15.17 delay 3:00 @15:41:15.23 SSV.dial CBV @15:41:15.26 CBV.dial SSV @15:41:16.13 Allowing 130s for 25.0ml of remaining 625.0ml @15:41:16.14 Intake.select :open @15:41:18.33 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:41:18.35 SS.reconfigure SSinhale.with maxSpeed: 26 @15:41:18.39 SS.seek 25ml @15:42:25.59 Stroke completed -- waiting for pressure to equalize ... @15:42:32.00 Intake.select :close @15:42:32.15 SSV.dial :exhaust @15:42:33.00 Exhaust.select :open @15:42:33.11 SS.reconfigure SSexhale @15:42:33.16 SS.seek 0ml @15:42:42.56 Exhaust.select :close @15:42:42.68 SSV.dial CBV @15:42:42.71 CBV.dial SSV @15:42:43.58 Allowing 130s for 25.0ml of remaining 600.0ml @15:42:43.59 Intake.select :open @15:42:46.35 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:42:46.37 SS.reconfigure SSinhale.with maxSpeed: 26 @15:42:46.42 SS.seek 25ml @15:43:53.57 Stroke completed -- waiting for pressure to equalize ... @15:43:59.97 Intake.select :close @15:44:00.14 SSV.dial :exhaust @15:44:01.00 Exhaust.select :open @15:44:01.11 SS.reconfigure SSexhale @15:44:01.17 SS.seek 0ml @15:44:10.82 Exhaust.select :close @15:44:10.93 SSV.dial CBV @15:44:10.96 CBV.dial SSV @15:44:11.85 Allowing 130s for 25.0ml of remaining 575.0ml @15:44:11.86 Intake.select :open @15:44:14.04 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:44:14.06 SS.reconfigure SSinhale.with maxSpeed: 26 @15:44:14.11 SS.seek 25ml @15:44:15.21 AFV.select :carrierSolution @15:44:15.32 delay 2 seconds @15:44:17.34 AS.reconfigure ASconfig @15:44:17.39 AS.seek 1ml @15:45:05.38 delay 1 second @15:45:06.39 AFV.select :holdingCoil @15:45:06.50 delay 1 second @15:45:07.52 ASV.dial :wasteD @15:45:08.55 AS.seek 0ml @15:45:19.30 Vacuum too high -- waiting up to 40s @15:45:20.94 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:45:20.96 SS.reconfigure SSinhale.with maxSpeed: 23 @15:45:21.01 SS.seek 25ml @15:45:23.76 Stroke completed -- waiting for pressure to equalize ... @15:45:28.91 Intake.select :close @15:45:29.07 SSV.dial :exhaust @15:45:29.94 Exhaust.select :open @15:45:30.06 SS.reconfigure SSexhale @15:45:30.11 SS.seek 0ml @15:45:39.99 Exhaust.select :close @15:45:40.11 SSV.dial CBV @15:45:40.14 CBV.dial SSV @15:45:41.00 Allowing 130s for 25.0ml of remaining 550.0ml @15:45:41.01 Intake.select :open @15:45:43.19 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:45:43.21 SS.reconfigure SSinhale.with maxSpeed: 23 @15:45:43.26 SS.seek 25ml @15:46:06.34 Priming Eluate @15:46:06.35 AFV.select :carrierSolution @15:46:06.46 delay 2 seconds @15:46:08.49 AS.seek 0.5ml @15:46:37.56 delay 1 second @15:46:38.57 AFV.select :holdingCoil @15:46:38.68 ATV.dial ASV @15:46:38.69 ASV.dial :SPErecovery @15:46:39.28 Swish #1 of 2 @15:46:39.36 AS.reconfigure ASslow0 @15:46:40.01 AS.seek 0.6ml @15:46:50.43 AS.seek 0.5ml @15:46:59.40 Stroke completed -- waiting for pressure to equalize ... @15:47:00.79 Swish #2 of 2 @15:47:00.88 AS.seek 0.6ml @15:47:05.80 Intake.select :close @15:47:05.97 SSV.dial :exhaust @15:47:06.82 Exhaust.select :open @15:47:06.93 SS.reconfigure SSexhale @15:47:06.98 SS.seek 0ml @15:47:11.35 AS.seek 0.5ml @15:47:16.13 Exhaust.select :close @15:47:16.25 SSV.dial CBV @15:47:16.31 CBV.dial SSV @15:47:17.14 Allowing 130s for 25.0ml of remaining 525.0ml @15:47:17.15 Intake.select :open @15:47:19.34 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:47:19.36 SS.reconfigure SSinhale.with maxSpeed: 23 @15:47:19.40 SS.seek 25ml @15:47:21.76 delay 3 seconds @15:47:24.85 AS.reconfigure ASslow1 @15:47:24.90 AS.seek 0.684ml @15:48:02.20 delay 3 seconds @15:48:05.23 Eluate primed @15:48:05.24 ATV.dial :waste2 @15:48:05.85 AS.reconfigure ASnorm @15:48:05.90 AS.seek 0ml @15:48:36.02 Stroke completed -- waiting for pressure to equalize ... @15:48:40.71 SPE.coast @15:48:40.80 ASV.dial :SPErecovery @15:48:40.82 Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1715h150ml.pcr @15:48:40.83 Building PCR Slug from ASV with MM1 mix, PP2 primer @15:48:40.85 Priming with carrier @15:48:40.86 ATV.dial ARV @15:48:41.90 ARV.dial :carrier @15:48:42.27 AS.seek 0.5ml @15:48:42.51 Intake.select :close @15:48:42.67 SSV.dial :exhaust @15:48:43.50 Exhaust.select :open @15:48:43.61 SS.reconfigure SSexhale @15:48:43.66 SS.seek 0ml @15:48:53.02 Exhaust.select :close @15:48:53.14 SSV.dial CBV @15:48:53.17 CBV.dial SSV @15:48:54.00 Allowing 130s for 25.0ml of remaining 500.0ml @15:48:54.01 Intake.select :open @15:48:56.20 Intake @34.8psia, vacuum from 20.0 to 24.0psi @15:48:56.22 SS.reconfigure SSinhale.with maxSpeed: 23 @15:48:56.26 SS.seek 25ml @15:49:07.82 AFV.select :carrierSolution @15:49:07.94 delay 2 seconds @15:49:09.97 AS.reconfigure ASconfig @15:49:10.02 AS.seek 1ml @15:49:39.06 delay 1 second @15:49:40.07 AFV.select :holdingCoil @15:49:40.64 delay 1 second @15:49:41.75 ATV.dial :waste @15:49:43.21 AS.reconfigure ASnorm @15:49:43.26 AS.seek :empty @15:50:12.23 Stroke completed -- waiting for pressure to equalize ... @15:50:19.28 Intake.select :close @15:50:19.44 SSV.dial :exhaust @15:50:20.27 Exhaust.select :open @15:50:20.38 SS.reconfigure SSexhale @15:50:20.44 SS.seek 0ml @15:50:29.40 Exhaust.select :close @15:50:29.52 SSV.dial CBV @15:50:29.55 CBV.dial SSV @15:50:30.39 Allowing 130s for 25.0ml of remaining 475.0ml @15:50:30.40 Intake.select :open @15:50:32.58 Intake @34.8psia, vacuum from 20.0 to 24.0psi @15:50:32.60 SS.reconfigure SSinhale.with maxSpeed: 23 @15:50:32.65 SS.seek 25ml @15:50:34.01 AFV.select :carrierSolution @15:50:34.13 delay 2 seconds @15:50:36.16 AS.reconfigure ASconfig @15:50:36.21 AS.seek 0.5ml @15:51:05.31 delay 1 second @15:51:06.32 AFV.select :holdingCoil @15:51:06.43 Building Slug @15:51:06.44 ATV.dial :ARV @15:51:07.99 delay 2 seconds @15:51:10.01 ARV.dial :air1 @15:51:10.42 delay 2 seconds @15:51:12.51 AS.reconfigure ASslow2 @15:51:12.56 AS.seek 0.506ml @15:51:18.91 delay 2 seconds @15:51:20.93 ARV.dial :MM1 @15:51:21.53 delay 2 seconds @15:51:23.56 Adding MM1 @15:51:23.63 AS.seek 0.524ml @15:51:42.10 delay 25 seconds @15:51:49.20 Stroke completed -- waiting for pressure to equalize ... @15:51:56.93 Intake.select :close @15:51:57.09 SSV.dial :exhaust @15:51:57.90 Exhaust.select :open @15:51:58.02 SS.reconfigure SSexhale @15:51:58.07 SS.seek 0ml @15:52:07.12 ARV.dial :PP2 @15:52:07.54 Adding PP2 @15:52:07.55 delay 2 seconds @15:52:07.58 Exhaust.select :close @15:52:07.70 SSV.dial CBV @15:52:07.73 CBV.dial SSV @15:52:08.58 Allowing 130s for 25.0ml of remaining 450.0ml @15:52:08.59 Intake.select :open @15:52:09.64 AS.seek 0.53ml @15:52:10.77 Intake @34.8psia, vacuum from 20.0 to 24.0psi @15:52:10.79 SS.reconfigure SSinhale.with maxSpeed: 23 @15:52:10.84 SS.seek 25ml @15:52:16.57 delay 10 seconds @15:52:26.60 ARV.dial :air2 @15:52:26.99 adding ARV air @15:52:27.01 delay 2 seconds @15:52:29.09 AS.seek 0.536ml @15:52:35.35 delay 5 seconds @15:52:40.37 ARV.dial :carrier @15:52:41.61 delay 7 seconds @15:52:48.70 AS.seek 0.594ml @15:53:26.64 Vacuum too high -- waiting up to 40s @15:53:28.54 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:53:28.56 SS.reconfigure SSinhale.with maxSpeed: 21 @15:53:28.61 SS.seek 25ml @15:53:29.56 Stroke completed -- waiting for pressure to equalize ... @15:53:35.34 Intake.select :close @15:53:35.50 SSV.dial :exhaust @15:53:36.33 Exhaust.select :open @15:53:36.44 SS.reconfigure SSexhale @15:53:36.49 SS.seek 0ml @15:53:45.79 Exhaust.select :close @15:53:45.91 SSV.dial CBV @15:53:45.94 CBV.dial SSV @15:53:46.80 Allowing 130s for 25.0ml of remaining 425.0ml @15:53:46.81 Intake.select :open @15:53:47.30 delay 5 seconds @15:53:49.04 Intake @34.8psia, vacuum from 20.0 to 24.0psi @15:53:49.06 SS.reconfigure SSinhale.with maxSpeed: 21 @15:53:49.11 SS.seek 25ml @15:53:52.31 ATV.dial :ASV @15:53:52.71 delay 2 seconds @15:53:54.80 AS.seek 0.6ml @15:54:01.28 delay 4 seconds @15:54:05.31 ATV.dial ARV @15:54:05.85 delay 2 seconds @15:54:07.87 ARV.dial :carrier @15:54:07.89 delay 2 seconds @15:54:09.98 AS.seek 0.65ml @15:55:00.61 delay 4 seconds @15:55:04.63 ATV.dial PCR @15:55:05.22 ASV.dial :waste1 @15:55:05.99 ARV.dial :air1 @15:55:06.38 Positioning Slug by 0.324ml @15:55:06.47 AS.seek 0.326ml @15:55:11.97 Stroke completed -- waiting for pressure to equalize ... @15:55:19.07 Intake.select :close @15:55:19.23 SSV.dial :exhaust @15:55:20.05 Exhaust.select :open @15:55:20.16 SS.reconfigure SSexhale @15:55:20.22 SS.seek 0ml @15:55:29.48 Exhaust.select :close @15:55:29.60 SSV.dial CBV @15:55:29.63 CBV.dial SSV @15:55:30.49 Allowing 130s for 25.0ml of remaining 400.0ml @15:55:30.50 Intake.select :open @15:55:32.68 Intake @34.8psia, vacuum from 20.0 to 24.0psi @15:55:32.70 SS.reconfigure SSinhale.with maxSpeed: 21 @15:55:32.75 SS.seek 25ml @15:56:56.01 Stroke completed -- waiting for pressure to equalize ... @15:57:04.34 Intake.select :close @15:57:04.51 SSV.dial :exhaust @15:57:05.35 Exhaust.select :open @15:57:05.46 SS.reconfigure SSexhale @15:57:05.51 SS.seek 0ml @15:57:14.81 Exhaust.select :close @15:57:14.93 SSV.dial CBV @15:57:14.96 CBV.dial SSV @15:57:15.80 Allowing 130s for 25.0ml of remaining 375.0ml @15:57:15.81 Intake.select :open @15:57:17.99 Intake @34.9psia, vacuum from 20.0 to 24.0psi @15:57:18.01 SS.reconfigure SSinhale.with maxSpeed: 21 @15:57:18.06 SS.seek 25ml @15:58:40.93 Stroke completed -- waiting for pressure to equalize ... @15:58:49.23 Intake.select :close @15:58:49.38 SSV.dial :exhaust @15:58:50.21 Exhaust.select :open @15:58:50.32 SS.reconfigure SSexhale @15:58:50.37 SS.seek 0ml @15:58:59.74 Exhaust.select :close @15:58:59.86 SSV.dial CBV @15:58:59.89 CBV.dial SSV @15:59:00.74 Allowing 130s for 25.0ml of remaining 350.0ml @15:59:00.75 Intake.select :open @15:59:02.93 Intake @34.9psia, vacuum from 20.0 to 24.0psi @15:59:03.49 SS.reconfigure SSinhale.with maxSpeed: 21 @15:59:03.63 SS.seek 25ml @16:00:02.89 Vacuum too high -- waiting up to 40s @16:00:04.76 Intake @34.7psia, vacuum from 20.0 to 24.0psi @16:00:04.78 SS.reconfigure SSinhale.with maxSpeed: 19 @16:00:04.83 SS.seek 25ml @16:00:31.91 Stroke completed -- waiting for pressure to equalize ... @16:00:32.58 delay 5 seconds @16:00:37.67 ATV.dial ARV @16:00:38.51 ASV.dial :PCRlock @16:00:38.96 delay 5 seconds @16:00:40.28 Intake.select :close @16:00:40.45 SSV.dial :exhaust @16:00:41.27 Exhaust.select :open @16:00:41.38 SS.reconfigure SSexhale @16:00:41.44 SS.seek 0ml @16:00:43.98 Running Ss Profile60C350 thermocycling @16:00:44.00 PCR.program Profile60C350 @16:00:44.14 Profile60C350 should complete in < 1:48:05 @16:00:44.15 PCR.run 2:20:06.25 @16:00:50.41 Exhaust.select :close @16:00:50.53 SSV.dial CBV @16:00:50.56 CBV.dial SSV @16:00:51.39 Allowing 130s for 25.0ml of remaining 325.0ml @16:00:51.40 Intake.select :open @16:00:53.58 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:00:53.61 SS.reconfigure SSinhale.with maxSpeed: 19 @16:00:53.65 SS.seek 25ml @16:02:25.71 Stroke completed -- waiting for pressure to equalize ... @16:02:35.32 Intake.select :close @16:02:35.48 SSV.dial :exhaust @16:02:36.32 Exhaust.select :open @16:02:36.43 SS.reconfigure SSexhale @16:02:36.49 SS.seek 0ml @16:02:45.57 Exhaust.select :close @16:02:45.69 SSV.dial CBV @16:02:45.72 CBV.dial SSV @16:02:46.58 Allowing 130s for 25.0ml of remaining 300.0ml @16:02:46.59 Intake.select :open @16:02:48.77 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:02:48.79 SS.reconfigure SSinhale.with maxSpeed: 19 @16:02:48.84 SS.seek 25ml @16:04:20.74 Stroke completed -- waiting for pressure to equalize ... @16:04:30.38 Intake.select :close @16:04:30.54 SSV.dial :exhaust @16:04:31.38 Exhaust.select :open @16:04:31.49 SS.reconfigure SSexhale @16:04:31.54 SS.seek 0ml @16:04:40.51 Exhaust.select :close @16:04:40.63 SSV.dial CBV @16:04:40.66 CBV.dial SSV @16:04:41.52 Allowing 130s for 25.0ml of remaining 275.0ml @16:04:41.53 Intake.select :open @16:04:43.72 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:04:43.74 SS.reconfigure SSinhale.with maxSpeed: 19 @16:04:43.79 SS.seek 25ml @16:06:14.08 Vacuum too high -- waiting up to 40s @16:06:16.24 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:06:16.26 SS.reconfigure SSinhale.with maxSpeed: 17 @16:06:16.31 SS.seek 25ml @16:06:18.73 Stroke completed -- waiting for pressure to equalize ... @16:06:27.05 Intake.select :close @16:06:27.22 SSV.dial :exhaust @16:06:28.07 Exhaust.select :open @16:06:28.18 SS.reconfigure SSexhale @16:06:28.23 SS.seek 0ml @16:06:37.57 Exhaust.select :close @16:06:37.69 SSV.dial CBV @16:06:37.72 CBV.dial SSV @16:06:38.57 Allowing 130s for 25.0ml of remaining 250.0ml @16:06:38.58 Intake.select :open @16:06:40.76 Intake @35.0psia, vacuum from 20.0 to 24.0psi @16:06:40.78 SS.reconfigure SSinhale.with maxSpeed: 17 @16:06:40.83 SS.seek 25ml @16:08:23.46 Stroke completed -- waiting for pressure to equalize ... @16:08:33.10 Intake.select :close @16:08:33.26 SSV.dial :exhaust @16:08:34.08 Exhaust.select :open @16:08:34.19 SS.reconfigure SSexhale @16:08:34.24 SS.seek 0ml @16:08:43.63 Exhaust.select :close @16:08:43.75 SSV.dial CBV @16:08:43.78 CBV.dial SSV @16:08:44.63 Allowing 130s for 25.0ml of remaining 225.0ml @16:08:44.64 Intake.select :open @16:08:46.82 Intake @35.0psia, vacuum from 20.0 to 24.0psi @16:08:46.84 SS.reconfigure SSinhale.with maxSpeed: 17 @16:08:46.89 SS.seek 25ml @16:10:29.82 Stroke completed -- waiting for pressure to equalize ... @16:10:40.66 Intake.select :close @16:10:40.82 SSV.dial :exhaust @16:10:41.67 Exhaust.select :open @16:10:41.78 SS.reconfigure SSexhale @16:10:41.83 SS.seek 0ml @16:10:51.29 Exhaust.select :close @16:10:51.41 SSV.dial CBV @16:10:51.44 CBV.dial SSV @16:10:52.30 Allowing 130s for 25.0ml of remaining 200.0ml @16:10:52.31 Intake.select :open @16:10:54.49 Intake @35.0psia, vacuum from 20.0 to 24.0psi @16:10:54.52 SS.reconfigure SSinhale.with maxSpeed: 17 @16:10:54.56 SS.seek 25ml @16:12:37.65 Stroke completed -- waiting for pressure to equalize ... @16:12:48.53 Intake.select :close @16:12:48.69 SSV.dial :exhaust @16:12:49.53 Exhaust.select :open @16:12:49.64 SS.reconfigure SSexhale @16:12:49.69 SS.seek 0ml @16:12:59.10 Exhaust.select :close @16:12:59.87 SSV.dial CBV @16:12:59.90 CBV.dial SSV @16:13:00.75 Allowing 130s for 25.0ml of remaining 175.0ml @16:13:00.76 Intake.select :open @16:13:02.94 Intake @35.0psia, vacuum from 20.0 to 24.0psi @16:13:02.96 SS.reconfigure SSinhale.with maxSpeed: 17 @16:13:03.01 SS.seek 25ml @16:14:18.39 Vacuum too high -- waiting up to 40s @16:14:20.56 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:14:20.58 SS.reconfigure SSinhale.with maxSpeed: 15 @16:14:20.63 SS.seek 25ml @16:14:51.95 Stroke completed -- waiting for pressure to equalize ... @16:15:02.23 Intake.select :close @16:15:02.39 SSV.dial :exhaust @16:15:03.22 Exhaust.select :open @16:15:03.33 SS.reconfigure SSexhale @16:15:03.38 SS.seek 0ml @16:15:12.59 Exhaust.select :close @16:15:12.71 SSV.dial CBV @16:15:12.74 CBV.dial SSV @16:15:13.58 Allowing 130s for 25.0ml of remaining 150.0ml @16:15:13.59 Intake.select :open @16:15:15.77 Intake @35.1psia, vacuum from 20.0 to 24.0psi @16:15:15.79 SS.reconfigure SSinhale.with maxSpeed: 15 @16:15:15.84 SS.seek 25ml @16:17:12.29 Stroke completed -- waiting for pressure to equalize ... @16:17:23.67 SS.reconfigure SSconfig @16:17:23.73 SS.hold @16:17:23.87 Intake.select :close @16:17:24.03 ShallowSampler::Clogged in WCR -- progress too slow after 1375.0ml @16:17:24.05 SSV.dial :exhaust @16:17:24.88 Exhaust.select :open @16:17:24.99 SS.reconfigure SSexhale @16:17:25.05 SS.seek 0ml @16:17:34.48 Exhaust.select :close @16:17:34.60 Sampled 1375.0ml @16:17:34.62 Drying puck before fixing for 4:00 @16:17:34.63 CBV.dial :waste2b @16:17:35.46 delay 1:00 @16:18:35.49 CSV.dial :air @16:18:35.97 CS.reconfigure CSfast @16:18:36.03 CS.seek 10ml @16:19:01.34 Pumping wcr puck from tube 3 up to 55psi... @16:19:01.35 CBV.dial SSV @16:19:02.74 CBV.dial between CSV and waste2b @16:19:03.82 CSV.dial between air and CTV @16:19:04.33 CS.seek 6.828ml @16:19:13.18 CTV.dial CSV,avoiding: :air @16:19:13.21 CSV.dial CTV @16:19:14.07 CS.reconfigure CSpressurize @16:19:14.13 CS.seek 0ml @16:19:32.14 CSV.dial :air @16:19:32.65 CS.reconfigure CSfast @16:19:32.71 CS.seek 10ml @16:19:57.71 CSV.dial between air and CTV @16:19:58.07 CS.seek 2.502ml @16:20:17.12 CTV.dial CSV,avoiding: :air @16:20:17.15 CSV.dial CTV @16:20:17.50 CS.reconfigure CSpressurize @16:20:17.56 CS.seek 0ml @16:20:22.11 CBV.dial :waste2b @16:20:23.06 CS.reconfigure CSconfig @16:20:23.11 CS.seek :empty @16:20:26.53 delay 1:00 @16:21:26.56 CTV.dial :air,avoiding: :intake @16:21:27.98 Fix #1 of 2 (with rnal) for 2:00 @16:21:28.00 CSV.dial CSR @16:21:28.54 Pulling 2ml of CSR.rnal @16:21:28.56 CSR.select :rnal @16:21:28.68 CS.seek 2ml @16:21:34.91 delay 5 seconds @16:21:39.93 CSR.select :air @16:21:40.06 CS.reconfigure CSfast @16:21:40.11 CS.seek 10ml @16:22:00.25 Pumping wcr puck from tube 3 up to 55psi... @16:22:00.26 CBV.dial SSV @16:22:01.66 CBV.dial between CSV and waste2b @16:22:02.73 CSV.dial between air and CTV @16:22:03.40 CS.seek 6.781ml @16:22:12.19 CTV.dial CSV,avoiding: :air @16:22:12.23 CSV.dial CTV @16:22:13.69 CS.reconfigure CSpressurize @16:22:13.75 CS.seek 0ml @16:22:31.75 CSV.dial :air @16:22:32.25 CS.reconfigure CSfast @16:22:32.31 CS.seek 10ml @16:22:57.22 CSV.dial between air and CTV @16:22:57.59 CS.seek 2.046ml @16:23:17.69 CTV.dial CSV,avoiding: :air @16:23:17.72 CSV.dial CTV @16:23:18.06 CS.reconfigure CSpressurize @16:23:18.12 CS.seek 0ml @16:23:19.55 delay 2:00 @16:25:19.57 CBV.dial :waste2b @16:25:19.94 CS.reconfigure CSconfig @16:25:19.99 CS.seek :empty @16:25:25.38 delay 1:00 @16:26:25.41 CTV.dial :air,avoiding: :intake @16:26:27.45 Fix #2 of 2 (with rnal) for 2:00 @16:26:27.46 CSV.dial CSR @16:26:28.00 Pulling 2ml of CSR.rnal @16:26:28.02 CSR.select :rnal @16:26:28.14 CS.seek 2ml @16:26:34.38 delay 5 seconds @16:26:39.40 CSR.select :air @16:26:39.53 CS.reconfigure CSfast @16:26:39.58 CS.seek 10ml @16:26:59.69 Pumping wcr puck from tube 3 up to 55psi... @16:26:59.70 CBV.dial SSV @16:27:01.10 CBV.dial between CSV and waste2b @16:27:02.15 CSV.dial between air and CTV @16:27:02.83 CS.seek 6.769ml @16:27:11.60 CTV.dial CSV,avoiding: :air @16:27:11.64 CSV.dial CTV @16:27:13.10 CS.reconfigure CSpressurize @16:27:13.16 CS.seek 0ml @16:27:30.87 delay 2:00 @16:29:30.89 CBV.dial :waste2b @16:29:31.26 CS.reconfigure CSconfig @16:29:31.31 CS.seek :empty @16:29:31.94 delay 1:00 @16:30:31.97 CTV.dial :air,avoiding: :intake @16:30:33.47 Storing wcr puck in Collection Clamp to tube 2 @16:30:33.66 SC.reconfigure SCtight @16:30:33.71 SC.seek 2 @16:30:35.87 SC.reconfigure SCloose @16:30:36.10 Elbow.reconfigure TightElbow @16:30:36.15 Elbow.seek 2 @16:30:38.02 Forearm.reconfigure TightForearm @16:30:38.07 Forearm.seek 2 + 700 counts @16:30:39.16 SE.reconfigure SEpick @16:30:39.23 SE.seek beamBreak @16:30:40.62 SE.configure SEconfig @16:30:40.79 Elbow.reconfigure LimpElbow @16:30:40.85 Forearm.reconfigure LooseForearm @16:30:40.90 Forearm.seek 2 @16:30:41.18 Elbow.hold @16:30:41.25 Elbow.reconfigure TightElbow @16:30:41.99 SE.seek 18.1 Pucks below Top Plate @16:30:42.48 Forearm.force -20 @16:30:42.53 Forearm.seek 2 + 349 counts @16:30:42.87 Forearm.reconfigure TightForearm @16:30:42.94 Forearm.seek 2 + 700 counts @16:30:43.33 SE.seek 19.1 Pucks below Top Plate @16:30:43.98 Forearm.reconfigure LooseForearm @16:30:44.04 Forearm.seek :retract @16:30:44.98 Elbow.seek CC @16:30:46.67 Elbow.reconfigure LooseElbow @16:30:46.78 Forearm.seek CC @16:30:47.77 Hand.close @16:30:48.48 CC.seek :open @16:30:53.02 Forearm.seek :retract @16:30:54.01 Elbow.reconfigure TightElbow @16:30:54.06 Elbow.seek 2 @16:30:55.92 Elbow.reconfigure LimpElbow @16:30:55.98 Forearm.seek 2 @16:30:56.99 Elbow.hold @16:30:57.07 Elbow.reconfigure TightElbow @16:30:57.12 Hand.open @16:30:57.98 Forearm.force -20 @16:30:58.03 Forearm.seek 2 + 344 counts @16:30:58.37 Forearm.reconfigure TightForearm @16:30:58.43 Forearm.seek 2 + 700 counts @16:30:58.81 SE.seek :down @16:30:59.80 SE.coast @16:30:59.98 Forearm.reconfigure LooseForearm @16:31:00.03 Forearm.seek :retract @16:31:00.96 SC.coast @16:31:01.00 Starting finalPurge @16:31:01.02 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:31:01.09 Hand.open @16:31:01.50 Elbow.seek FlushPuck::Garage @16:31:03.52 Elbow.reconfigure LooseElbow @16:31:03.63 Forearm.seek :garage @16:31:04.64 Hand.close @16:31:05.37 Forearm.seek :retract @16:31:06.94 Elbow.reconfigure TightElbow @16:31:07.08 Elbow.seek CC @16:31:09.99 Elbow.reconfigure LooseElbow @16:31:10.09 Forearm.reconfigure TightForearm @16:31:10.14 Forearm.seek CC @16:31:11.43 CC.seek :closed @16:31:15.68 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.09 @16:31:15.77 Hand.open @16:31:16.43 Forearm.reconfigure LooseForearm @16:31:16.48 Forearm.seek :retract @16:31:17.50 Flushing the Collection Valve::Manifold @16:31:17.64 CSV.dial CSR @16:31:18.18 Pulling 5ml of CSR.flush @16:31:18.19 CSR.select :flush @16:31:18.32 CS.seek 5ml @16:31:32.04 delay 5 seconds @16:31:37.05 CSR.select :air @16:31:37.23 CS.reconfigure CSfast @16:31:37.28 CS.seek 7.5ml @16:31:44.25 CSV.dial :waste @16:31:45.65 CS.reconfigure CSconfig @16:31:45.70 CS.seek 0ml @16:32:05.79 Flushing lower manifold's waste1 port @16:32:05.93 CSV.dial :flush @16:32:06.42 CS.reconfigure CSfast @16:32:06.47 CS.seek 5ml @16:32:19.38 delay 5 seconds @16:32:24.40 CSV.dial :air @16:32:24.95 CS.seek 7ml @16:32:30.70 CSV.dial CTV @16:32:30.73
CTV.dial CSV @16:32:32.21 CBV.dial :waste1 @16:32:33.05 CS.seek 0ml @16:32:50.67 Flushing lower manifold's waste2b port @16:32:50.81 CSV.dial :flush @16:32:51.61 CS.seek 5ml @16:33:04.60 delay 5 seconds @16:33:09.61 CSV.dial :air @16:33:10.20 CS.seek 7ml @16:33:16.07 CSV.dial CTV @16:33:16.10
CTV.dial CSV @16:33:16.59 CBV.dial :waste2b @16:33:17.42 CS.seek 0ml @16:33:35.04 Flushing upper manifold @16:33:35.18 CSV.dial :flush @16:33:35.99 CS.seek 5ml @16:33:48.95 delay 5 seconds @16:33:54.50 CSV.dial :air @16:33:55.08 CS.seek 7ml @16:34:01.14 CSV.dial CBV @16:34:01.17
CBV.dial CSV @16:34:02.35 CTV.dial :air @16:34:03.77 CS.seek 0ml @16:34:21.54 delay 7 seconds @16:34:28.56 CS.seek 3ml @16:34:36.63 CSV.dial :waste @16:34:37.15 CS.seek 0ml @16:34:45.44 CSV.dial :flush @16:34:45.94 CS.seek 3ml @16:34:54.07 delay 5 seconds @16:34:59.08 CSV.dial :air @16:34:59.68 CS.seek 5ml @16:35:05.41 CSV.dial :air @16:35:05.44 CS.seek 0ml @16:35:18.32 delay 7 seconds @16:35:25.35 CS.seek 3ml @16:35:33.53 CSV.dial :waste @16:35:34.37 CS.seek 0ml @16:35:42.51 Disinfecting external Intake with mfbkill @16:35:42.65 CSV.dial CSR @16:35:44.12 Pulling 8ml of CSR.mfbkill @16:35:44.13 CSR.select :mfbkill @16:35:44.26 CS.seek 8ml @16:36:04.38 delay 5 seconds @16:36:09.40 CSR.select :air @16:36:09.54 CS.seek 10ml @16:36:15.32 CSV.dial CBV @16:36:15.35
CBV.dial CSV @16:36:16.46 CTV.dial :intake @16:36:17.25 Intake.select :open @16:36:17.38 CS.seek 2ml @16:36:38.41 Intake.select :close @16:36:38.53 CTV.dial :air @16:36:39.34 CS.reconfigure CSconfig @16:36:39.39 CS.seek 0ml @16:36:45.51 Flushing lower manifold's waste1 port @16:36:45.52 CSV.dial CSR @16:36:46.68 Pulling 4ml of CSR.mfbkill @16:36:46.69 CSR.select :mfbkill @16:36:46.83 CS.seek 4ml @16:36:58.04 delay 5 seconds @16:37:03.05 CSR.select :air @16:37:03.18 CS.reconfigure CSfast @16:37:03.23 CS.seek 10ml @16:37:18.61 CSV.dial CTV @16:37:18.64
CTV.dial CSV @16:37:20.15 CBV.dial :waste1 @16:37:20.65 CS.seek 0ml @16:37:45.51 Flushing lower manifold's waste2b port @16:37:45.52 CSV.dial CSR @16:37:46.07 Pulling 4ml of CSR.mfbkill @16:37:46.08 CSR.select :mfbkill @16:37:46.21 CS.reconfigure CSconfig @16:37:46.25 CS.seek 4ml @16:37:57.45 delay 5 seconds @16:38:02.46 CSR.select :air @16:38:02.63 CS.reconfigure CSfast @16:38:02.67 CS.seek 10ml @16:38:17.86 CSV.dial CTV @16:38:17.89
CTV.dial CSV @16:38:18.40 CBV.dial :waste2b @16:38:19.24 CS.seek 0ml @16:38:43.98 Killing upper manifold @16:38:43.99 CSV.dial CSR @16:38:44.54 Pulling 4ml of CSR.mfbkill @16:38:44.55 CSR.select :mfbkill @16:38:44.68 CS.reconfigure CSconfig @16:38:44.72 CS.seek 4ml @16:38:55.95 delay 5 seconds @16:39:00.97 CSR.select :air @16:39:01.11 CS.reconfigure CSfast @16:39:01.15 CS.seek 10ml @16:39:16.33 CSV.dial CBV @16:39:16.36
CBV.dial CSV @16:39:17.50 CTV.dial :air @16:39:18.93 CS.seek 0ml @16:39:43.80 delay 7 seconds @16:39:50.83 CS.seek 3ml @16:39:58.97 CSV.dial :waste @16:39:59.59 CS.seek 0ml @16:40:07.70 Flushing upper manifold @16:40:07.84 CSV.dial :flush @16:40:08.34 CS.seek 5ml @16:40:21.14 delay 5 seconds @16:40:26.17 CSV.dial :air @16:40:26.73 CS.seek 7ml @16:40:32.61 CSV.dial CBV @16:40:32.64
CBV.dial CSV @16:40:33.75 CTV.dial :air @16:40:33.77 CS.seek 0ml @16:40:51.39 delay 7 seconds @16:40:58.41 CS.seek 3ml @16:41:06.54 CSV.dial :waste @16:41:07.05 CS.seek 0ml @16:41:15.25 CSV.dial :flush @16:41:15.75 CS.seek 3ml @16:41:23.83 delay 5 seconds @16:41:28.84 CSV.dial :air @16:41:29.40 CS.seek 5ml @16:41:35.29 CSV.dial :air @16:41:35.32 CS.seek 0ml @16:41:48.14 delay 7 seconds @16:41:55.16 CS.seek 3ml @16:42:03.26 CSV.dial :waste @16:42:04.09 CS.seek 0ml @16:42:12.17 Flushing lower manifold's waste1 port @16:42:12.31 CSV.dial :flush @16:42:12.81 CS.seek 5ml @16:42:25.89 delay 5 seconds @16:42:30.90 CSV.dial :air @16:42:32.08 CS.seek 7ml @16:42:37.96 CSV.dial CTV @16:42:37.99
CTV.dial CSV @16:42:39.49 CBV.dial :waste1 @16:42:40.01 CS.seek 0ml @16:42:57.61 Flushing lower manifold's waste2b port @16:42:57.75 CSV.dial :flush @16:42:58.56 CS.seek 5ml @16:43:11.52 delay 5 seconds @16:43:16.54 CSV.dial :air @16:43:17.12 CS.seek 7ml @16:43:22.99 CSV.dial CTV @16:43:23.02
CTV.dial CSV @16:43:23.50 CBV.dial :waste2b @16:43:24.36 CS.seek 0ml @16:43:42.02 Disinfecting external Intake with kill @16:43:42.16 CSV.dial CSR @16:43:42.70 Pulling 8ml of CSR.kill @16:43:42.71 CSR.select :kill @16:43:42.84 CS.seek 8ml @16:44:02.89 delay 5 seconds @16:44:07.91 CSR.select :air @16:44:08.05 CS.seek 10ml @16:44:13.77 CSV.dial CBV @16:44:13.80
CBV.dial CSV @16:44:14.94 CTV.dial :intake @16:44:15.74 Intake.select :open @16:44:15.86 CS.seek 2ml @16:44:37.01 Intake.select :close @16:44:37.13 CTV.dial :air @16:44:37.94 CS.reconfigure CSconfig @16:44:37.99 CS.seek 0ml @16:44:44.07 CSV.dial CSR @16:44:45.24 Pulling 5ml of CSR.flush @16:44:45.25 CSR.select :flush @16:44:45.38 CS.seek 5ml @16:44:59.15 delay 5 seconds @16:45:04.16 CSR.select :air @16:45:04.30 CS.seek 10ml @16:45:17.98 CSV.dial CBV @16:45:19.10 CS.seek 0ml @16:45:45.58 Garaging FlushPuck in Collection Clamp @16:45:45.66 Elbow.reconfigure TightElbow @16:45:45.71 Elbow.seek CC @16:45:45.97 Elbow.reconfigure LooseElbow @16:45:46.08 Forearm.seek CC @16:45:47.07 Hand.close @16:45:47.88 CC.seek :open @16:45:52.24 Forearm.seek :retract @16:45:53.25 Elbow.reconfigure TightElbow @16:45:53.30 Elbow.seek :garage @16:45:56.39 Elbow.reconfigure LooseElbow @16:45:56.50 Forearm.reconfigure TightForearm @16:45:56.55 Forearm.seek :garage @16:45:57.74 Hand.open @16:45:59.07 Forearm.reconfigure LooseForearm @16:45:59.12 Forearm.seek :retract @16:46:00.16 Starting initialPurge @16:46:00.17 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:46:00.25 Hand.open @16:46:00.59 Elbow.reconfigure TightElbow @16:46:00.64 Elbow.seek FlushPuck::Garage @16:46:00.90 Elbow.reconfigure LooseElbow @16:46:01.00 Forearm.seek :garage @16:46:02.02 Hand.close @16:46:02.77 Forearm.seek :retract @16:46:03.77 Elbow.reconfigure TightElbow @16:46:03.82 Elbow.seek CC @16:46:06.73 Elbow.reconfigure LooseElbow @16:46:06.84 Forearm.reconfigure TightForearm @16:46:06.89 Forearm.seek CC @16:46:08.17 CC.seek :closed @16:46:12.42 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.08 @16:46:12.49 Hand.open @16:46:13.20 Forearm.reconfigure LooseForearm @16:46:13.24 Forearm.seek :retract @16:46:14.22 Flushing the Collection Valve::Manifold @16:46:14.36 CSV.dial CSR @16:46:15.53 Pulling 5ml of CSR.flush @16:46:15.54 CSR.select :flush @16:46:15.67 CS.seek 5ml @16:46:29.49 delay 5 seconds @16:46:34.50 CSR.select :air @16:46:34.68 CS.reconfigure CSfast @16:46:34.73 CS.seek 7.501ml @16:46:41.73 CSV.dial :waste @16:46:43.13 CS.reconfigure CSconfig @16:46:43.18 CS.seek 0ml @16:47:03.33 Purging external Intake @16:47:03.46 CSV.dial :flush @16:47:03.96 CS.reconfigure CSfast @16:47:04.01 CS.seek 10ml @16:47:28.65 delay 5 seconds @16:47:33.67 CSV.dial CBV @16:47:33.71
CBV.dial CSV @16:47:34.52 CTV.dial :intake @16:47:35.34 Intake.select :open @16:47:35.46 CS.seek 0ml @16:48:01.33 Intake.select :close @16:48:01.45 CTV.dial :air @16:48:02.27 Flushing upper manifold @16:48:02.40 CSV.dial :flush @16:48:03.20 CS.seek 5ml @16:48:16.27 delay 5 seconds @16:48:21.29 CSV.dial :air @16:48:21.84 CS.seek 6.999ml @16:48:27.62 CSV.dial CBV @16:48:28.28
CBV.dial CSV @16:48:29.40 CTV.dial :air @16:48:29.42 CS.seek 0ml @16:48:46.99 delay 7 seconds @16:48:54.04 CS.seek 3ml @16:49:02.11 CSV.dial :waste @16:49:02.63 CS.seek 0ml @16:49:10.89 CSV.dial :flush @16:49:11.38 CS.seek 3ml @16:49:19.49 delay 5 seconds @16:49:24.50 CSV.dial :air @16:49:25.06 CS.seek 5ml @16:49:30.83 CSV.dial :air @16:49:30.86 CS.seek 0ml @16:49:43.68 delay 7 seconds @16:49:50.70 CS.seek 3ml @16:49:58.86 CSV.dial :waste @16:49:59.68 CS.seek 0ml @16:50:07.87 Garaging FlushPuck in Collection Clamp @16:50:07.95 Elbow.reconfigure TightElbow @16:50:08.00 Elbow.seek CC @16:50:08.25 Elbow.reconfigure LooseElbow @16:50:08.39 Forearm.seek CC @16:50:09.39 Hand.close @16:50:10.01 CC.seek :open @16:50:14.37 Forearm.seek :retract @16:50:15.35 Elbow.reconfigure TightElbow @16:50:15.40 Elbow.seek :garage @16:50:18.44 Elbow.reconfigure LooseElbow @16:50:18.55 Forearm.reconfigure TightForearm @16:50:18.59 Forearm.seek :garage @16:50:19.73 Hand.open @16:50:20.45 Forearm.reconfigure LooseForearm @16:50:20.49 Forearm.seek :retract @16:50:21.51 Completed [mbaPhase] begun 14:01:45 @16:50:21.53 Preparing to sleep until Fri Oct 19 14:01:45 +0200 2018 @16:50:21.58 deferred until MFBPCR finish @16:50:21.60
Waiting for MFBPCR til 14:01CEST19-Oct-18 @16:50:21.62 Continuing at 14:01CEST19-Oct-18 @16:50:21.68 GoodNews.email "Then, in tube 3: mbaPhase\n/home/esp/esp2/mission/18tundeployv3.rb:123\n{1 => 22, 2 => 20, 3 => 2}\nCan@16:50:21, 14.4C, 71% humidity, 14.3psia, 12.891V, 0.701A, 0.701A avg, 9.04W",Subject: "Waiting until 14:01CEST19-Oct-18" @17:23:35.93 PCR.off @17:23:37.96 PCR for Ss completed -- closed /var/log/esp/Ss18oct1715h150ml.pcr @17:23:38.04 Cleaning PCR @17:23:38.05 AFV.select :carrierSolution @17:23:38.16 delay 2 seconds @17:23:40.19 AS.reconfigure ASconfig @17:23:40.24 AS.seek 0.5ml @17:23:50.55 delay 1 second @17:23:51.56 AFV.select :holdingCoil @17:23:51.67 ASV.dial :waste1 @17:23:52.06 ATV.dial :air3 @17:23:53.29 AS.reconfigure ASslow1 @17:23:53.34 AS.seek 0.51ml @17:23:55.64 delay 2 seconds @17:23:57.66 ATV.dial :bleach @17:23:58.27 AS.seek 0.81ml @17:24:58.84 delay 3 seconds @17:25:01.86 ATV.dial :air3 @17:25:02.39 delay 3 seconds @17:25:05.48 AS.seek 0.835ml @17:25:10.77 delay 3 seconds @17:25:13.79 ATV.dial :bleach @17:25:14.40 AS.seek 0.82ml @17:25:17.69 delay 3 seconds @17:25:20.71 ARV.dial :waste @17:25:21.91 delay 3 seconds @17:25:24.93 ATV.dial :ARV @17:25:25.90 AS.reconfigure ASconfig @17:25:25.95 AS.seek 0.47ml @17:25:46.35 AFV.select :carrierSolution @17:25:46.47 delay 2 seconds @17:25:48.50 AS.seek 1ml @17:26:19.30 delay 1 second @17:26:20.31 AFV.select :holdingCoil @17:26:20.43 delay 1 second @17:26:21.46 AS.reconfigure ASfast @17:26:21.51 AS.seek 0ml @17:26:48.86 AFV.select :carrierSolution @17:26:48.98 delay 2 seconds @17:26:51.01 AS.reconfigure ASconfig @17:26:51.06 AS.seek 1ml @17:27:48.86 delay 1 second @17:27:49.87 AFV.select :holdingCoil @17:27:49.98 delay 1 second @17:27:51.01 AS.reconfigure ASfast @17:27:51.06 AS.seek 0ml @17:28:18.41 AFV.select :carrierSolution @17:28:18.53 delay 2 seconds @17:28:20.56 AS.reconfigure ASconfig @17:28:20.61 AS.seek 1ml @17:29:18.41 delay 1 second @17:29:19.42 AFV.select :holdingCoil @17:29:19.53 delay 1 second @17:29:20.56 AS.reconfigure ASfast @17:29:20.61 AS.seek 0ml @17:29:47.97 ARV.dial between carrier and air1 @17:29:48.45 ATV.dial :air3 @17:29:49.66 AS.reconfigure ASslow1 @17:29:49.71 AS.seek 0.01ml @17:29:52.05 delay 2 seconds @17:29:54.06 ATV.dial :bleach @17:29:54.68 AS.seek 0.81ml @17:32:35.83 delay 3 seconds @17:32:38.85 ATV.dial :air3 @17:32:39.38 delay 3 seconds @17:32:42.47 AS.seek 0.835ml @17:32:47.70 delay 3 seconds @17:32:50.72 ATV.dial :bleach @17:32:51.32 AS.seek 0.82ml @17:32:54.61 delay 3 seconds @17:32:57.63 ATV.dial PCR @17:32:58.03 AS.reconfigure ASconfig @17:32:58.08 AS.seek :empty @17:33:45.52 PCR.program :bleachProfile @17:33:45.59 PCR program should complete in < 2:45 @17:33:45.60 PCR.run 10:00 @17:36:56.51 PCR.off @17:36:56.53 AFV.select :carrierSolution @17:36:56.64 delay 2 seconds @17:36:58.67 AS.seek 1ml @17:37:56.50 delay 1 second @17:37:57.51 AFV.select :holdingCoil @17:37:57.62 delay 1 second @17:37:58.65 AS.reconfigure ASfast @17:37:58.70 AS.seek 0ml @17:38:26.03 delay 3 seconds @17:38:29.05 AFV.select :carrierSolution @17:38:29.16 delay 2 seconds @17:38:31.19 AS.reconfigure ASconfig @17:38:31.24 AS.seek 1ml @17:39:28.99 delay 1 second @17:39:30.00 AFV.select :holdingCoil @17:39:30.11 delay 1 second @17:39:31.14 AS.reconfigure ASfast @17:39:31.19 AS.seek 0ml @17:39:58.54 delay 3 seconds @17:40:01.56 AFV.select :carrierSolution @17:40:01.67 delay 2 seconds @17:40:03.70 AS.reconfigure ASconfig @17:40:03.76 AS.seek 1ml @17:41:01.55 delay 1 second @17:41:02.56 AFV.select :holdingCoil @17:41:02.67 delay 1 second @17:41:03.70 AS.reconfigure ASfast @17:41:03.75 AS.seek 0ml @17:41:31.11 delay 3 seconds @17:41:34.13 AFV.select :carrierSolution @17:41:34.24 delay 2 seconds @17:41:36.27 AS.reconfigure ASconfig @17:41:36.33 AS.seek 1ml @17:42:34.13 delay 1 second @17:42:35.14 AFV.select :holdingCoil @17:42:35.25 delay 1 second @17:42:36.28 AS.reconfigure ASfast @17:42:36.33 AS.seek 0ml @17:43:03.69 delay 3 seconds @17:43:06.71 AFV.select :carrierSolution @17:43:06.82 delay 2 seconds @17:43:08.85 AS.reconfigure ASconfig @17:43:08.91 AS.seek 1ml @17:44:06.67 delay 1 second @17:44:07.69 AFV.select :holdingCoil @17:44:07.80 delay 1 second @17:44:08.83 AS.reconfigure ASnorm @17:44:08.88 AS.seek 0ml @17:44:59.68 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1717h150ml.pcr @17:44:59.70 Building PCR Slug from ASV with MM2 mix, PP4 primer @17:44:59.72 Priming with carrier @17:44:59.73 ATV.dial ARV @17:45:00.30 ARV.dial :carrier @17:45:00.65 AS.seek 0.5ml @17:45:26.18 AFV.select :carrierSolution @17:45:26.30 delay 2 seconds @17:45:28.33 AS.reconfigure ASconfig @17:45:28.38 AS.seek 1ml @17:45:57.40 delay 1 second @17:45:58.41 AFV.select :holdingCoil @17:45:58.52 delay 1 second @17:45:59.54 ATV.dial :waste @17:46:01.19 AS.reconfigure ASnorm @17:46:01.24 AS.seek :empty @17:46:51.98 AFV.select :carrierSolution @17:46:52.10 delay 2 seconds @17:46:54.13 AS.reconfigure ASconfig @17:46:54.18 AS.seek 0.5ml @17:47:23.29 delay 1 second @17:47:24.30 AFV.select :holdingCoil @17:47:24.41 Building Slug @17:47:24.42 ATV.dial :ARV @17:47:25.93 delay 2 seconds @17:47:27.95 ARV.dial :air1 @17:47:28.36 delay 2 seconds @17:47:30.44 AS.reconfigure ASslow2 @17:47:30.50 AS.seek 0.506ml @17:47:36.76 delay 2 seconds @17:47:38.79 ARV.dial :MM2 @17:47:40.19 delay 2 seconds @17:47:42.22 Adding MM2 @17:47:42.29 AS.seek 0.524ml @17:48:00.71 delay 25 seconds @17:48:25.73 ARV.dial :PP4 @17:48:26.12 Adding PP4 @17:48:26.14 delay 2 seconds @17:48:28.22 AS.seek 0.53ml @17:48:34.62 delay 10 seconds @17:48:44.65 ARV.dial :air3 @17:48:45.04 adding ARV air @17:48:45.05 delay 2 seconds @17:48:47.14 AS.seek 0.536ml @17:48:53.63 delay 5 seconds @17:48:58.65 ARV.dial :carrier @17:48:59.85 delay 7 seconds @17:49:06.94 AS.seek 0.594ml @17:50:05.59 delay 5 seconds @17:50:10.61 ATV.dial :ASV @17:50:11.15 delay 2 seconds @17:50:13.24 AS.seek 0.6ml @17:50:19.69 delay 4 seconds @17:50:23.71 ATV.dial ARV @17:50:24.23 delay 2 seconds @17:50:26.25 ARV.dial :carrier @17:50:26.27 delay 2 seconds @17:50:28.36 AS.seek 0.65ml @17:51:18.76 delay 4 seconds @17:51:22.78 ATV.dial PCR @17:51:23.37 ASV.dial :waste1 @17:51:23.38 ARV.dial :air1 @17:51:23.78 Positioning Slug by 0.324ml @17:51:23.87 AS.seek 0.326ml @17:56:50.10 delay 5 seconds @17:56:55.12 ATV.dial ARV @17:56:55.87 ASV.dial :PCRlock @17:56:56.26 delay 5 seconds @17:57:01.29 Running Tt1 Profile60C300 thermocycling @17:57:01.30 PCR.program Profile60C300 @17:57:01.44 Profile60C300 should complete in < 1:45:21 @17:57:01.45 PCR.run 2:16:41.25 @19:19:02.88 PCR.off @19:19:04.44 PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1717h150ml.pcr @19:19:04.51 Cleaning PCR @19:19:04.52 AFV.select :carrierSolution @19:19:04.63 delay 2 seconds @19:19:06.66 AS.reconfigure ASconfig @19:19:06.71 AS.seek 0.5ml @19:19:17.02 delay 1 second @19:19:18.03 AFV.select :holdingCoil @19:19:18.14 ASV.dial :waste1 @19:19:18.52 ATV.dial :air3 @19:19:19.72 AS.reconfigure ASslow1 @19:19:19.78 AS.seek 0.51ml @19:19:22.07 delay 2 seconds @19:19:24.09 ATV.dial :bleach @19:19:24.70 AS.seek 0.81ml @19:20:25.29 delay 3 seconds @19:20:28.31 ATV.dial :air3 @19:20:29.46 delay 3 seconds @19:20:32.55 AS.seek 0.835ml @19:20:37.84 delay 3 seconds @19:20:40.86 ATV.dial :bleach @19:20:41.48 AS.seek 0.82ml @19:20:44.79 delay 3 seconds @19:20:47.81 ARV.dial :waste @19:20:48.40 delay 3 seconds @19:20:51.42 ATV.dial :ARV @19:20:52.25 AS.reconfigure ASconfig @19:20:52.31 AS.seek 0.47ml @19:21:12.68 AFV.select :carrierSolution @19:21:12.80 delay 2 seconds @19:21:14.83 AS.seek 1ml @19:21:45.59 delay 1 second @19:21:46.60 AFV.select :holdingCoil @19:21:46.71 delay 1 second @19:21:47.74 AS.reconfigure ASfast @19:21:47.79 AS.seek 0ml @19:22:15.12 AFV.select :carrierSolution @19:22:15.24 delay 2 seconds @19:22:17.27 AS.reconfigure ASconfig @19:22:17.32 AS.seek 1ml @19:23:15.10 delay 1 second @19:23:16.11 AFV.select :holdingCoil @19:23:16.22 delay 1 second @19:23:17.25 AS.reconfigure ASfast @19:23:17.31 AS.seek 0ml @19:23:44.64 AFV.select :carrierSolution @19:23:44.76 delay 2 seconds @19:23:46.79 AS.reconfigure ASconfig @19:23:46.84 AS.seek 1ml @19:24:44.63 delay 1 second @19:24:45.64 AFV.select :holdingCoil @19:24:45.75 delay 1 second @19:24:46.78 AS.reconfigure ASfast @19:24:46.83 AS.seek 0ml @19:25:14.15 ARV.dial between carrier and air1 @19:25:14.63 ATV.dial :air3 @19:25:15.84 AS.reconfigure ASslow1 @19:25:15.89 AS.seek 0.01ml @19:25:18.24 delay 2 seconds @19:25:20.25 ATV.dial :bleach @19:25:20.84 AS.seek 0.81ml @19:28:01.99 delay 3 seconds @19:28:05.01 ATV.dial :air3 @19:28:05.54 delay 3 seconds @19:28:08.63 AS.seek 0.835ml @19:28:13.91 delay 3 seconds @19:28:16.93 ATV.dial :bleach @19:28:17.55 AS.seek 0.82ml @19:28:20.84 delay 3 seconds @19:28:23.86 ATV.dial PCR @19:28:24.24 AS.reconfigure ASconfig @19:28:24.29 AS.seek :empty @19:29:11.77 PCR.program :bleachProfile @19:29:11.82 PCR program should complete in < 2:45 @19:29:11.83 PCR.run 10:00 @19:32:22.74 PCR.off @19:32:22.77 AFV.select :carrierSolution @19:32:22.89 delay 2 seconds @19:32:24.92 AS.seek 1ml @19:33:22.76 delay 1 second @19:33:23.77 AFV.select :holdingCoil @19:33:23.88 delay 1 second @19:33:24.91 AS.reconfigure ASfast @19:33:24.96 AS.seek 0ml @19:33:52.31 delay 3 seconds @19:33:55.33 AFV.select :carrierSolution @19:33:55.44 delay 2 seconds @19:33:57.47 AS.reconfigure ASconfig @19:33:57.53 AS.seek 1ml @19:34:55.30 delay 1 second @19:34:56.32 AFV.select :holdingCoil @19:34:56.43 delay 1 second @19:34:57.46 AS.reconfigure ASfast @19:34:57.51 AS.seek 0ml @19:35:24.86 delay 3 seconds @19:35:27.88 AFV.select :carrierSolution @19:35:27.99 delay 2 seconds @19:35:30.02 AS.reconfigure ASconfig @19:35:30.07 AS.seek 1ml @19:36:27.87 delay 1 second @19:36:28.88 AFV.select :holdingCoil @19:36:28.99 delay 1 second @19:36:30.02 AS.reconfigure ASfast @19:36:30.07 AS.seek 0ml @19:36:57.42 delay 3 seconds @19:37:00.45 AFV.select :carrierSolution @19:37:00.56 delay 2 seconds @19:37:02.59 AS.reconfigure ASconfig @19:37:02.65 AS.seek 1ml @19:38:00.45 delay 1 second @19:38:01.46 AFV.select :holdingCoil @19:38:01.57 delay 1 second @19:38:03.22 AS.reconfigure ASfast @19:38:03.27 AS.seek 0ml @19:38:30.61 delay 3 seconds @19:38:33.63 AFV.select :carrierSolution @19:38:33.74 delay 2 seconds @19:38:35.77 AS.reconfigure ASconfig @19:38:35.83 AS.seek 1ml @19:39:33.61 delay 1 second @19:39:34.62 AFV.select :holdingCoil @19:39:34.73 delay 1 second @19:39:35.76 AS.reconfigure ASnorm @19:39:35.81 AS.seek 0ml @19:40:26.55 Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1719h150ml.pcr @19:40:26.56 Building PCR Slug from ASV with MM1 mix, PP1 primer @19:40:26.58 Priming with carrier @19:40:26.59 ATV.dial ARV @19:40:27.28 ARV.dial :carrier @19:40:27.63 AS.seek 0.5ml @19:40:53.19 AFV.select :carrierSolution @19:40:53.31 delay 2 seconds @19:40:55.34 AS.reconfigure ASconfig @19:40:55.39 AS.seek 1ml @19:41:24.42 delay 1 second @19:41:25.43 AFV.select :holdingCoil @19:41:25.54 delay 1 second @19:41:26.56 ATV.dial :waste @19:41:28.11 AS.reconfigure ASnorm @19:41:28.16 AS.seek :empty @19:42:18.88 AFV.select :carrierSolution @19:42:19.00 delay 2 seconds @19:42:21.03 AS.reconfigure ASconfig @19:42:21.08 AS.seek 0.5ml @19:42:50.17 delay 1 second @19:42:51.18 AFV.select :holdingCoil @19:42:51.29 Building Slug @19:42:51.30 ATV.dial :ARV @19:42:52.92 delay 2 seconds @19:42:54.94 ARV.dial :air1 @19:42:55.34 delay 2 seconds @19:42:57.42 AS.reconfigure ASslow2 @19:42:57.48 AS.seek 0.506ml @19:43:03.75 delay 2 seconds @19:43:05.77 ARV.dial :MM1 @19:43:06.34 delay 2 seconds @19:43:08.37 Adding MM1 @19:43:08.44 AS.seek 0.524ml @19:43:27.01 delay 25 seconds @19:43:52.03 ARV.dial :PP1 @19:43:52.42 Adding PP1 @19:43:52.44 delay 2 seconds @19:43:54.52 AS.seek 0.53ml @19:44:00.94 delay 10 seconds @19:44:10.96 ARV.dial :air1 @19:44:11.34 adding ARV air @19:44:11.35 delay 2 seconds @19:44:13.44 AS.seek 0.536ml @19:44:19.62 delay 5 seconds @19:44:24.64 ARV.dial :carrier @19:44:25.05 delay 7 seconds @19:44:32.14 AS.seek 0.594ml @19:45:30.62 delay 5 seconds @19:45:35.64 ATV.dial :ASV @19:45:36.05 delay 2 seconds @19:45:38.14 AS.seek 0.6ml @19:45:44.33 delay 4 seconds @19:45:48.35 ATV.dial ARV @19:45:48.89 delay 2 seconds @19:45:50.91 ARV.dial :carrier @19:45:50.93 delay 2 seconds @19:45:53.02 AS.seek 0.65ml @19:46:43.58 delay 4 seconds @19:46:47.60 ATV.dial PCR @19:46:48.19 ASV.dial :waste1 @19:46:48.20 ARV.dial :air1 @19:46:48.60 Positioning Slug by 0.324ml @19:46:48.69 AS.seek 0.326ml @19:52:14.70 delay 5 seconds @19:52:19.72 ATV.dial ARV @19:52:20.56 ASV.dial :PCRlock @19:52:20.94 delay 5 seconds @19:52:25.97 Running IPc ProfileIPc thermocycling @19:52:25.98 PCR.program ProfileIPc @19:52:26.14 ProfileIPc should complete in < 1:50:49 @19:52:26.15 PCR.run 2:23:31.25 @21:16:45.38 PCR.off @21:16:48.04 PCR for IPc completed -- closed /var/log/esp/IPc18oct1719h150ml.pcr @21:16:48.06 Full MFB Decontamination @21:16:48.07 Decon extraction column and decon AIV @21:16:48.10 SPE.hold 2:46:40,55C @21:16:48.15 ASV.dial :SPEdelivery @21:16:48.77 delay 20 seconds @21:17:08.79 AFV.select :carrierSolution @21:17:08.90 delay 2 seconds @21:17:10.93 AS.reconfigure ASconfig @21:17:10.98 AS.seek 0.5ml @21:17:21.31 delay 1 second @21:17:22.32 AFV.select :holdingCoil @21:17:22.43 ATV.dial :air1 @21:17:24.38 AS.reconfigure ASslow1 @21:17:24.43 AS.seek 0.52ml @21:17:28.74 ATV.dial :ASV @21:17:30.26 ASV.dial :SPErecovery @21:17:30.93 AS.seek 0.62ml @21:17:51.32 ATV.dial :waste @21:17:53.22 AS.reconfigure ASnorm @21:17:53.27 AS.seek :empty @21:18:24.91 AFV.select :holdingCoil @21:18:24.93 ATV.dial :waste @21:18:24.95 AS.reconfigure ASconfig @21:18:25.00 AS.seek 0ml @21:18:25.32 AFV.select :carrierSolution @21:18:25.44 delay 2 seconds @21:18:27.47 AS.seek 0.5ml @21:18:56.50 delay 1 second @21:18:57.51 AFV.select :holdingCoil @21:18:57.62 ATV.dial :air3 @21:18:58.30 AS.reconfigure ASslow1 @21:18:58.36 AS.seek 0.52ml @21:19:02.66 ATV.dial :air3 @21:19:02.75 AS.seek 0.53ml @21:19:05.01 delay 2 seconds @21:19:07.03 ATV.dial :bleach @21:19:07.51 AS.seek 0.71ml @21:19:44.00 delay 3 seconds @21:19:47.02 ATV.dial :air3 @21:19:47.58 delay 3 seconds @21:19:50.67 AS.seek 0.735ml @21:19:55.99 delay 3 seconds @21:19:59.01 ATV.dial :bleach @21:19:59.65 AS.seek 0.72ml @21:20:02.94 delay 3 seconds @21:20:05.97 ATV.dial ASV @21:20:07.02 ASV.dial :SPErecovery @21:20:07.10 AS.seek 0.365ml @21:21:18.74 ATV.dial :waste @21:21:20.41 AS.reconfigure ASnorm @21:21:20.46 AS.seek :empty @21:21:39.24 ATV.dial :air3 @21:21:39.91 AS.reconfigure ASslow1 @21:21:39.97 AS.seek 0.009ml @21:21:42.44 delay 2 seconds @21:21:44.45 ATV.dial :bleach @21:21:44.92 AS.seek 0.579ml @21:23:39.83 delay 3 seconds @21:23:42.85 ATV.dial :air3 @21:23:43.38 delay 3 seconds @21:23:46.47 AS.seek 0.604ml @21:23:51.78 delay 3 seconds @21:23:54.81 ATV.dial :bleach @21:23:55.40 AS.seek 0.589ml @21:23:58.72 delay 3 seconds @21:24:01.74 ATV.dial :ASV @21:24:02.73 ASV.dial :wasteR @21:24:03.13 AS.seek 0ml @21:26:01.86 delay 1:00 @21:27:01.88 ATV.dial ASV @21:27:01.90 AFV.select :carrierSolution @21:27:02.00 delay 2 seconds @21:27:04.03 AS.reconfigure ASconfig @21:27:04.08 AS.seek 1ml @21:28:01.86 delay 1 second @21:28:02.87 AFV.select :holdingCoil @21:28:02.98 delay 1 second @21:28:04.00 ASV.dial :wasteR @21:28:04.64 AS.reconfigure ASslow1 @21:28:04.70 AS.seek 0ml @21:31:26.10 delay 10 seconds @21:31:36.13 ATV.dial :air3 @21:31:37.58 AS.seek 0.01ml @21:31:39.90 delay 2 seconds @21:31:41.91 ATV.dial :bleach @21:31:42.54 AS.seek 0.58ml @21:33:37.47 delay 3 seconds @21:33:40.49 ATV.dial :air3 @21:33:41.04 delay 3 seconds @21:33:44.13 AS.seek 0.605ml @21:33:49.42 delay 3 seconds @21:33:52.44 ATV.dial :bleach @21:33:53.06 AS.seek 0.59ml @21:33:56.35 delay 3 seconds @21:33:59.38 ATV.dial ASV @21:34:00.37 ASV.dial :SPEdelivery @21:34:00.73 AS.seek 0ml @21:35:59.62 delay 1:00 @21:36:59.64 AFV.select :carrierSolution @21:36:59.75 delay 2 seconds @21:37:01.78 AS.reconfigure ASconfig @21:37:01.83 AS.seek 1ml @21:37:59.63 delay 1 second @21:38:00.64 AFV.select :holdingCoil @21:38:00.75 delay 1 second @21:38:01.77 ATV.dial ASV @21:38:01.79 ASV.dial :SPEdelivery @21:38:01.81 AS.reconfigure ASslow1 @21:38:01.86 AS.seek 0ml @21:41:23.23 delay 10 seconds @21:41:33.25 ATV.dial :air2 @21:41:34.05 AS.reconfigure ASslow0 @21:41:34.11 AS.seek 0.8ml @21:42:54.84 delay 3 seconds @21:42:57.86 ATV.dial :ASV @21:42:58.81 ASV.dial :SPEdelivery @21:42:58.84 AS.reconfigure ASslow1 @21:42:58.89 AS.seek 0.03ml @21:45:34.02 delay 20 seconds @21:45:54.05 ATV.dial :waste,via: :air3 @21:45:55.67 AS.reconfigure ASconfig @21:45:55.72 AS.seek :empty @21:45:57.78 SPE.coast @21:45:57.87 Removing Bleach from Mixing Coil @21:45:57.88 AFV.select :carrierSolution @21:45:57.99 delay 2 seconds @21:46:00.02 AS.seek 0.5ml @21:46:29.13 delay 1 second @21:46:30.14 AFV.select :holdingCoil @21:46:30.25 ATV.dial :ASV @21:46:31.85 ASV.dial :SPErecovery @21:46:32.46 AS.reconfigure ASslow1 @21:46:32.52 AS.seek 0.73ml @21:47:19.01 ASV.dial :wasteD @21:47:19.57 AS.reconfigure ASnorm @21:47:19.63 AS.seek 0ml @21:47:56.73 AFV.select :carrierSolution @21:47:56.85 delay 2 seconds @21:47:58.88 AS.reconfigure ASconfig @21:47:58.93 AS.seek 0.5ml @21:48:27.96 delay 1 second @21:48:28.97 AFV.select :holdingCoil @21:48:29.08 ATV.dial ASV @21:48:29.09 ASV.dial :SPErecovery @21:48:29.74 AS.reconfigure ASslow0 @21:48:29.79 AS.seek 0.3ml @21:48:50.22 ATV.dial :air2 @21:48:51.33 AS.reconfigure ASslow1 @21:48:51.38 AS.seek 0.32ml @21:48:55.74 ATV.dial ASV @21:48:56.72 AS.reconfigure ASslow0 @21:48:56.77 AS.seek 0.57ml @21:49:22.16 ASV.dial :wasteD @21:49:22.74 AS.reconfigure ASnorm @21:49:22.80 AS.seek 0ml @21:49:51.84 AFV.select :carrierSolution @21:49:51.96 delay 2 seconds @21:49:53.99 AS.reconfigure ASconfig @21:49:54.04 AS.seek 0.5ml @21:50:23.17 delay 1 second @21:50:24.18 AFV.select :holdingCoil @21:50:24.29 ATV.dial ASV @21:50:24.30 ASV.dial :SPErecovery @21:50:24.94 AS.reconfigure ASslow0 @21:50:24.99 AS.seek 0.3ml @21:50:45.41 ATV.dial :air2 @21:50:46.51 AS.reconfigure ASslow1 @21:50:46.57 AS.seek 0.32ml @21:50:51.52 ATV.dial ASV @21:50:52.52 AS.reconfigure ASslow0 @21:50:52.57 AS.seek 0.57ml @21:51:18.05 ASV.dial :wasteD @21:51:18.71 AS.reconfigure ASnorm @21:51:18.77 AS.seek 0ml @21:51:47.83 ATV.dial :air2 @21:51:48.76 AS.reconfigure ASslow0 @21:51:48.82 AS.seek 0.3ml @21:52:19.34 ATV.dial :ASV @21:52:20.26 ASV.dial :wasteD @21:52:20.28 AS.reconfigure ASconfig @21:52:20.33 AS.seek 0.05ml @21:52:35.02 ATV.dial :waste @21:52:36.79 AS.seek 0ml @21:52:39.95 ASV.dial :SPEdelivery @21:52:40.98 deconLysateINT @21:52:40.99 ATV.dial :air3 @21:52:41.75 AS.reconfigure ASslow1 @21:52:41.80 AS.seek 0.01ml @21:52:44.14 delay 2 seconds @21:52:46.15 ATV.dial :bleach @21:52:46.63 AS.seek 0.96ml @21:55:58.07 delay 3 seconds @21:56:01.09 ATV.dial :air3 @21:56:01.62 delay 3 seconds @21:56:04.71 AS.seek 0.985ml @21:56:10.04 delay 3 seconds @21:56:13.06 ATV.dial :bleach @21:56:13.66 AS.seek 0.97ml @21:56:16.96 delay 3 seconds @21:56:19.99 ATV.dial AIV @21:56:21.26 AIV.dial :coilToMFB @21:56:21.27 AWV.select :waste @21:56:21.28 AFV.select :carrierSolution @21:56:21.40 delay 2 seconds @21:56:23.43 AS.reconfigure ASconfig @21:56:23.48 AS.seek 1ml @21:56:25.51 delay 1 second @21:56:26.52 AFV.select :holdingCoil @21:56:26.63 delay 1 second @21:56:27.66 AS.reconfigure ASslow0 @21:56:27.70 AS.seek :empty @21:58:08.72 AFV.select :carrierSolution @21:58:08.84 delay 2 seconds @21:58:10.87 AS.reconfigure ASconfig @21:58:10.92 AS.seek 1ml @21:59:08.73 delay 1 second @21:59:09.74 AFV.select :holdingCoil @21:59:09.85 delay 1 second @21:59:10.88 AS.reconfigure ASslow0 @21:59:10.93 AS.seek :empty @22:00:51.88 AFV.select :carrierSolution @22:00:52.00 delay 2 seconds @22:00:54.03 AS.reconfigure ASconfig @22:00:54.08 AS.seek 1ml @22:01:51.89 delay 1 second @22:01:52.90 AFV.select :holdingCoil @22:01:53.01 delay 1 second @22:01:54.04 AS.reconfigure ASslow0 @22:01:54.09 AS.seek :empty @22:03:35.04 ATV.dial :air2 @22:03:35.71 AS.reconfigure ASslow1 @22:03:35.76 AS.seek 0.009ml @22:03:38.09 AS.reconfigure ASslow0 @22:03:38.14 AS.seek 1ml @22:05:18.08 ATV.dial AIV @22:05:18.82 AS.seek 0.02ml @22:06:57.65 ATV.dial :waste @22:06:59.20 AS.seek 0ml @22:07:01.50 AWV.select :air @22:07:01.67 Cleaning PCR @22:07:01.68 AFV.select :carrierSolution @22:07:01.79 delay 2 seconds @22:07:03.82 AS.reconfigure ASconfig @22:07:03.87 AS.seek 0.5ml @22:07:32.91 delay 1 second @22:07:33.92 AFV.select :holdingCoil @22:07:34.03 ASV.dial :waste1 @22:07:34.41 ATV.dial :air3 @22:07:35.06 AS.reconfigure ASslow1 @22:07:35.12 AS.seek 0.51ml @22:07:37.41 delay 2 seconds @22:07:39.43 ATV.dial :bleach @22:07:39.90 AS.seek 0.81ml @22:08:40.48 delay 3 seconds @22:08:43.50 ATV.dial :air3 @22:08:44.03 delay 3 seconds @22:08:47.12 AS.seek 0.835ml @22:08:53.03 delay 3 seconds @22:08:56.05 ATV.dial :bleach @22:08:56.65 AS.seek 0.82ml @22:08:59.95 delay 3 seconds @22:09:02.97 ARV.dial :waste @22:09:03.56 delay 3 seconds @22:09:06.58 ATV.dial :ARV @22:09:07.54 AS.reconfigure ASconfig @22:09:07.59 AS.seek 0.47ml @22:09:28.01 AFV.select :carrierSolution @22:09:28.13 delay 2 seconds @22:09:30.16 AS.seek 1ml @22:10:00.91 delay 1 second @22:10:01.92 AFV.select :holdingCoil @22:10:02.03 delay 1 second @22:10:03.06 AS.reconfigure ASfast @22:10:03.11 AS.seek 0ml @22:10:30.46 AFV.select :carrierSolution @22:10:30.58 delay 2 seconds @22:10:32.61 AS.reconfigure ASconfig @22:10:32.67 AS.seek 1ml @22:11:30.47 delay 1 second @22:11:31.48 AFV.select :holdingCoil @22:11:31.59 delay 1 second @22:11:32.62 AS.reconfigure ASfast @22:11:32.68 AS.seek 0ml @22:12:00.01 AFV.select :carrierSolution @22:12:00.13 delay 2 seconds @22:12:02.16 AS.reconfigure ASconfig @22:12:02.21 AS.seek 1ml @22:13:00.02 delay 1 second @22:13:01.03 AFV.select :holdingCoil @22:13:01.14 delay 1 second @22:13:02.17 AS.reconfigure ASfast @22:13:02.22 AS.seek 0ml @22:13:29.55 ARV.dial between carrier and air1 @22:13:30.05 ATV.dial :air3 @22:13:31.26 AS.reconfigure ASslow1 @22:13:31.31 AS.seek 0.01ml @22:13:33.66 delay 2 seconds @22:13:35.67 ATV.dial :bleach @22:13:36.27 AS.seek 0.81ml @22:16:17.47 delay 3 seconds @22:16:20.49 ATV.dial :air3 @22:16:21.02 delay 3 seconds @22:16:24.11 AS.seek 0.835ml @22:16:29.50 delay 3 seconds @22:16:32.52 ATV.dial :bleach @22:16:33.12 AS.seek 0.82ml @22:16:36.39 delay 3 seconds @22:16:39.41 ATV.dial PCR @22:16:39.81 AS.reconfigure ASconfig @22:16:39.86 AS.seek :empty @22:17:27.32 PCR.program :bleachProfile @22:17:27.37 PCR program should complete in < 2:45 @22:17:27.39 PCR.run 10:00 @22:20:47.39 PCR.off @22:20:47.41 AFV.select :carrierSolution @22:20:47.53 delay 2 seconds @22:20:49.56 AS.seek 1ml @22:21:47.41 delay 1 second @22:21:48.42 AFV.select :holdingCoil @22:21:48.53 delay 1 second @22:21:49.56 AS.reconfigure ASfast @22:21:49.61 AS.seek 0ml @22:22:16.96 delay 3 seconds @22:22:19.98 AFV.select :carrierSolution @22:22:20.09 delay 2 seconds @22:22:22.12 AS.reconfigure ASconfig @22:22:22.17 AS.seek 1ml @22:23:19.97 delay 1 second @22:23:20.98 AFV.select :holdingCoil @22:23:21.09 delay 1 second @22:23:22.12 AS.reconfigure ASfast @22:23:22.17 AS.seek 0ml @22:23:49.52 delay 3 seconds @22:23:52.54 AFV.select :carrierSolution @22:23:52.65 delay 2 seconds @22:23:54.68 AS.reconfigure ASconfig @22:23:54.75 AS.seek 1ml @22:24:52.55 delay 1 second @22:24:53.56 AFV.select :holdingCoil @22:24:53.67 delay 1 second @22:24:54.70 AS.reconfigure ASfast @22:24:54.75 AS.seek 0ml @22:25:22.08 delay 3 seconds @22:25:25.10 AFV.select :carrierSolution @22:25:25.21 delay 2 seconds @22:25:27.24 AS.reconfigure ASconfig @22:25:27.29 AS.seek 1ml @22:26:25.07 delay 1 second @22:26:26.08 AFV.select :holdingCoil @22:26:26.19 delay 1 second @22:26:27.22 AS.reconfigure ASfast @22:26:27.27 AS.seek 0ml @22:26:55.24 delay 3 seconds @22:26:58.26 AFV.select :carrierSolution @22:26:58.37 delay 2 seconds @22:27:00.40 AS.reconfigure ASconfig @22:27:00.46 AS.seek 1ml @22:27:58.24 delay 1 second @22:27:59.25 AFV.select :holdingCoil @22:27:59.36 delay 1 second @22:28:00.39 AS.reconfigure ASnorm @22:28:00.44 AS.seek 0ml @22:28:51.16 AFV.select :carrierSolution @22:28:51.27 delay 2 seconds @22:28:53.30 AS.reconfigure ASconfig @22:28:53.35 AS.seek 1ml @22:29:51.15 delay 1 second @22:29:52.16 AFV.select :holdingCoil @22:29:52.27 delay 1 second @22:29:53.29 ATV.dial :waste @22:29:54.44 AS.reconfigure ASnorm @22:29:54.50 AS.seek 0.5ml @22:30:20.07 ATV.dial :waste2 @22:30:21.27 AS.seek 0ml @22:30:46.80 AS.coast @22:30:46.84 SS.coast @22:30:46.88 CC.coast @22:30:46.92 CS.coast @22:30:46.96 PC.coast @22:30:47.00 PS.coast @22:30:47.05 Forearm.coast @22:30:47.09 Elbow.coast @22:30:47.48 Gate.power :core,:OFF @22:30:47.49 Gate.power :raw,:OFF @22:30:47.50 Shutdown @22:30:47.52 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @09:11:46.59CEST18-Oct-18 Session Begun @09:11:53.26 -> ESP.wakeup @09:11:53.32 Powering core bus @09:11:58.29 Gate.power :raw,:ON @09:11:58.98 SPE.coast @09:11:59.07 SPE.configure SPEconfig @09:11:59.19 AS.configure ASconfig @09:11:59.31 SS.configure SSconfig @09:11:59.43 CC.configure CCconfig @09:11:59.56 CS.configure CSconfig @09:11:59.69 PC.configure PCconfig @09:11:59.81 PS.configure PSconfig @09:11:59.95 Forearm.configure LooseForearm @09:12:00.08 Elbow.configure LooseElbow @09:12:00.22 SC.configure SCloose @09:12:00.35 SE.configure SEconfig @09:12:00.43 CH.coast @09:12:00.50 CH.configure CHconfig @09:12:00.56 PH.coast @09:12:00.65 PH.configure PHconfig @09:12:00.72 PCR.off @09:12:00.76 PCR.pidtemp= 50,0.1,0,300,25,25 @09:12:00.82 PCR.caltemp= 2.7073,0.0373,0 @09:12:00.87 All dwarf controllers running firmware version 3.75 @09:12:01.00 Valve.reset @09:12:01.20 PCR.off @09:12:01.25 PCR.pidtemp= 50,0.1,0,300,25,25 @09:12:01.29 PCR.caltemp= 2.7073,0.0373,0 @09:12:01.46 Intake.select :close @09:12:01.51 Exhaust.select :close @09:12:01.57 CTV.dial :air @09:12:01.69 PSV.dial :air @09:12:01.85 CSV.dial :waste1 @09:12:02.46 Forearm.reconfigure LooseForearm @09:12:02.58 Forearm.home.seek :retract @09:12:02.69 SSV.dial :spit,avoiding: :exhaust @09:12:02.71 PS.reconfigure PSconfig @09:12:02.85 PS.home.seek 0ml @09:12:03.36 Hand.close @09:12:03.40 Elbow.jog 1500 @09:12:03.71 CS.reconfigure CSfast @09:12:03.75 SS.reconfigure SSconfig @09:12:03.87 CS.home.seek 0ml @09:12:03.91 SS.home.seek 0ml @09:12:04.06 Elbow.reconfigure LooseElbow @09:12:04.13 Elbow.home.seek :clear @09:12:07.61 SE.reconfigure SEconfig @09:12:07.71 SE.home.seek :down @09:12:08.45 SC.reconfigure SCsloppy @09:12:08.53 SC.home.seek :initial @09:12:11.99 PS.coast @09:12:12.54 Forearm.seek :retract @09:12:12.66 Elbow.seek :hammer @09:12:13.83 Forearm.reconfigure TightForearm @09:12:13.91 Forearm.seek 5 + 350 counts @09:12:15.01 Forearm.seek :retract @09:12:16.27 PC.reconfigure PCconfig @09:12:16.29 CC.reconfigure CCconfig @09:12:16.41 PC.home.seek :open @09:12:16.46 CC.home.seek :open @09:12:30.03 -> ESP.ready? @09:12:42.93 -> MFB.ready? @09:12:50.11 -> MFB.ready! @09:12:50.21 Readying the MFB @09:12:50.23 Homing MFB Syringe @09:12:50.26 AFV.select :holdingCoil @09:12:50.44 ATV.dial :air2 @09:12:51.11 AS.reconfigure ASconfig @09:12:51.18 AS.home.seek :home @09:12:56.72 AFV.select :carrierSolution @09:12:56.86 delay 2 seconds @09:12:58.89 AS.seek 1ml @09:13:51.79 delay 1 second @09:13:52.80 AFV.select :holdingCoil @09:13:52.94 delay 1 second @09:13:53.97 ATV.dial :waste @09:13:54.99 AS.reconfigure ASnorm @09:13:55.07 AS.home.seek :empty @09:14:47.65 ATV.dial :waste @09:14:47.67 ARV.dial between carrier and air1 @09:14:47.78 ASV.dial :waste1 @09:17:19.25 -> pcrDecon @09:17:19.35 Cleaning PCR @09:17:19.37 AFV.select :carrierSolution @09:17:19.51 delay 2 seconds @09:17:21.54 AS.reconfigure ASconfig @09:17:21.61 AS.seek 0.5ml @09:17:50.74 delay 1 second @09:17:51.75 AFV.select :holdingCoil @09:17:51.88 ASV.dial :waste1 @09:17:51.89 ATV.dial :air3 @09:17:52.58 AS.reconfigure ASslow1 @09:17:52.65 AS.seek 0.51ml @09:17:54.96 delay 2 seconds @09:17:56.98 ATV.dial :bleach @09:17:57.44 AS.seek 0.81ml @09:18:58.04 delay 3 seconds @09:19:01.06 ATV.dial :air3 @09:19:01.60 delay 3 seconds @09:19:04.68 AS.seek 0.835ml @09:19:10.01 delay 3 seconds @09:19:13.04 ATV.dial :bleach @09:19:13.64 AS.seek 0.82ml @09:19:16.95 delay 3 seconds @09:19:19.97 ARV.dial :waste @09:19:20.48 delay 3 seconds @09:19:23.50 ATV.dial :ARV @09:19:24.39 AS.reconfigure ASconfig @09:19:24.46 AS.seek 0.47ml @09:19:44.86 AFV.select :carrierSolution @09:19:45.00 delay 2 seconds @09:19:47.62 AS.seek 1ml @09:20:18.39 delay 1 second @09:20:19.40 AFV.select :holdingCoil @09:20:19.54 delay 1 second @09:20:20.57 AS.reconfigure ASfast @09:20:20.64 AS.seek 0ml @09:20:47.98 AFV.select :carrierSolution @09:20:48.12 delay 2 seconds @09:20:50.15 AS.reconfigure ASconfig @09:20:50.22 AS.seek 1ml @09:21:47.98 delay 1 second @09:21:49.00 AFV.select :holdingCoil @09:21:49.14 delay 1 second @09:21:50.17 AS.reconfigure ASfast @09:21:50.24 AS.seek 0ml @09:22:17.59 AFV.select :carrierSolution @09:22:17.73 delay 2 seconds @09:22:19.76 AS.reconfigure ASconfig @09:22:19.83 AS.seek 1ml @09:23:17.65 delay 1 second @09:23:18.66 AFV.select :holdingCoil @09:23:18.80 delay 1 second @09:23:19.83 AS.reconfigure ASfast @09:23:19.90 AS.seek 0ml @09:23:47.25 ARV.dial between carrier and air1 @09:23:47.76 ATV.dial :air3 @09:23:49.00 AS.reconfigure ASslow1 @09:23:49.07 AS.seek 0.01ml @09:23:51.42 delay 2 seconds @09:23:53.43 ATV.dial :bleach @09:23:54.05 AS.seek 0.81ml @09:26:35.22 delay 3 seconds @09:26:38.24 ATV.dial :air3 @09:26:38.79 delay 3 seconds @09:26:41.88 AS.seek 0.835ml @09:26:47.23 delay 3 seconds @09:26:50.25 ATV.dial :bleach @09:26:50.86 AS.seek 0.82ml @09:26:54.17 delay 3 seconds @09:26:57.19 ATV.dial PCR @09:26:57.61 AS.reconfigure ASconfig @09:26:57.68 AS.seek :empty @09:27:45.17 PCR.program :bleachProfile @09:27:45.25 PCR program should complete in < 2:45 @09:27:45.27 PCR.run 10:00 @09:31:20.45 PCR.off @09:31:20.49 AFV.select :carrierSolution @09:31:20.63 delay 2 seconds @09:31:22.66 AS.seek 1ml @09:32:20.51 delay 1 second @09:32:21.52 AFV.select :holdingCoil @09:32:21.65 delay 1 second @09:32:22.68 AS.reconfigure ASfast @09:32:22.76 AS.seek 0ml @09:32:50.12 delay 3 seconds @09:32:53.14 AFV.select :carrierSolution @09:32:53.27 delay 2 seconds @09:32:55.30 AS.reconfigure ASconfig @09:32:55.38 AS.seek 1ml @09:33:53.19 delay 1 second @09:33:54.21 AFV.select :holdingCoil @09:33:54.36 delay 1 second @09:33:55.39 AS.reconfigure ASfast @09:33:55.46 AS.seek 0ml @09:34:22.83 delay 3 seconds @09:34:25.86 AFV.select :carrierSolution @09:34:26.01 delay 2 seconds @09:34:28.04 AS.reconfigure ASconfig @09:34:28.11 AS.seek 1ml @09:35:25.92 delay 1 second @09:35:25.94 Errno::EPIPE in mwj's_reader -- Broken pipe @09:35:25.96 Session Ended @09:41:42.83 Session Begun @09:41:52.41 -> showlog 20 @09:42:21.94 -> showlog 50 @09:42:58.92 -> AS @09:43:02.37 -> ATV @09:43:35.85 -> AFV @09:43:47.77 -> AFV.holdingCoil @09:43:47.79 AFV.select :holdingCoil @09:43:56.74 -> ATV @09:44:24.92 -> ASV @09:44:30.03 -> AS.empty @09:44:30.05 AS.seek :empty @09:45:39.77 -> ATV.waste @09:45:39.78 ATV.dial :waste @09:45:44.64 -> exit @09:45:44.66 Session Ended @09:46:25.86 Session Begun @09:46:35.12 -> MFB.ready? @09:46:40.11 -> pcrDecon @09:46:40.22 Cleaning PCR @09:46:40.23 AFV.select :carrierSolution @09:46:40.38 delay 2 seconds @09:46:42.42 AS.seek 0.5ml @09:47:11.53 delay 1 second @09:47:12.54 AFV.select :holdingCoil @09:47:12.67 ASV.dial :waste1 @09:47:12.69 ATV.dial :air3 @09:47:13.52 AS.reconfigure ASslow1 @09:47:13.60 AS.seek 0.51ml @09:47:15.88 delay 2 seconds @09:47:17.89 ATV.dial :bleach @09:47:18.37 AS.seek 0.81ml @09:48:19.00 delay 3 seconds @09:48:22.02 ATV.dial :air3 @09:48:22.57 delay 3 seconds @09:48:25.66 AS.seek 0.835ml @09:48:30.96 delay 3 seconds @09:48:33.99 ATV.dial :bleach @09:48:34.61 AS.seek 0.82ml @09:48:37.94 delay 3 seconds @09:48:41.51 ARV.dial :waste @09:48:42.02 delay 3 seconds @09:48:45.04 ATV.dial :ARV @09:48:45.92 AS.reconfigure ASconfig @09:48:45.99 AS.seek 0.47ml @09:49:06.38 AFV.select :carrierSolution @09:49:06.52 delay 2 seconds @09:49:08.55 AS.seek 1ml @09:49:39.32 delay 1 second @09:49:40.34 AFV.select :holdingCoil @09:49:40.47 delay 1 second @09:49:41.50 AS.reconfigure ASfast @09:49:41.57 AS.seek 0ml @09:50:08.92 AFV.select :carrierSolution @09:50:09.06 delay 2 seconds @09:50:11.09 AS.reconfigure ASconfig @09:50:11.16 AS.seek 1ml @09:51:08.96 delay 1 second @09:51:09.98 AFV.select :holdingCoil @09:51:10.12 delay 1 second @09:51:11.15 AS.reconfigure ASfast @09:51:11.22 AS.seek 0ml @09:51:38.59 AFV.select :carrierSolution @09:51:38.74 delay 2 seconds @09:51:40.77 AS.reconfigure ASconfig @09:51:40.84 AS.seek 1ml @09:52:38.66 delay 1 second @09:52:39.67 AFV.select :holdingCoil @09:52:39.80 delay 1 second @09:52:40.83 AS.reconfigure ASfast @09:52:40.90 AS.seek 0ml @09:53:08.26 ARV.dial between carrier and air1 @09:53:08.77 ATV.dial :air3 @09:53:10.01 AS.reconfigure ASslow1 @09:53:10.09 AS.seek 0.01ml @09:53:12.47 delay 2 seconds @09:53:14.48 ATV.dial :bleach @09:53:15.09 AS.seek 0.81ml @09:55:03.88 Session Begun @09:55:51.94 -> showlog 10 @09:55:56.26 delay 3 seconds @09:55:59.28 ATV.dial :air3 @09:55:59.85 delay 3 seconds @09:56:02.94 AS.seek 0.835ml @09:56:08.26 delay 3 seconds @09:56:11.28 ATV.dial :bleach @09:56:11.89 AS.seek 0.82ml @09:56:15.23 delay 3 seconds @09:56:18.25 ATV.dial PCR @09:56:18.69 AS.reconfigure ASconfig @09:56:18.77 AS.seek :empty @09:57:06.26 PCR.program :bleachProfile @09:57:06.35 PCR program should complete in < 2:45 @09:57:06.37 PCR.run 10:00 @10:00:32.47 PCR.off @10:00:32.53 AFV.select :carrierSolution @10:00:32.67 delay 2 seconds @10:00:34.71 AS.seek 1ml @10:01:32.59 delay 1 second @10:01:33.60 AFV.select :holdingCoil @10:01:33.74 delay 1 second @10:01:34.77 AS.reconfigure ASfast @10:01:34.85 AS.seek 0ml @10:02:02.34 delay 3 seconds @10:02:05.36 AFV.select :carrierSolution @10:02:05.50 delay 2 seconds @10:02:07.53 AS.reconfigure ASconfig @10:02:07.62 AS.seek 1ml @10:03:05.49 delay 1 second @10:03:06.50 AFV.select :holdingCoil @10:03:06.64 delay 1 second @10:03:07.67 AS.reconfigure ASfast @10:03:07.75 AS.seek 0ml @10:03:35.15 delay 3 seconds @10:03:38.17 AFV.select :carrierSolution @10:03:38.32 delay 2 seconds @10:03:40.35 AS.reconfigure ASconfig @10:03:40.44 AS.seek 1ml @10:04:38.21 delay 1 second @10:04:39.22 AFV.select :holdingCoil @10:04:39.36 delay 1 second @10:04:40.39 AS.reconfigure ASfast @10:04:40.48 AS.seek 0ml @10:05:07.85 delay 3 seconds @10:05:10.87 AFV.select :carrierSolution @10:05:11.02 delay 2 seconds @10:05:13.05 AS.reconfigure ASconfig @10:05:13.14 AS.seek 1ml @10:06:10.95 delay 1 second @10:06:11.96 AFV.select :holdingCoil @10:06:12.11 delay 1 second @10:06:13.14 AS.reconfigure ASfast @10:06:13.23 AS.seek 0ml @10:06:40.60 delay 3 seconds @10:06:43.62 AFV.select :carrierSolution @10:06:43.77 delay 2 seconds @10:06:45.80 AS.reconfigure ASconfig @10:06:45.90 AS.seek 1ml @10:07:43.73 delay 1 second @10:07:44.74 AFV.select :holdingCoil @10:07:44.88 delay 1 second @10:07:45.91 AS.reconfigure ASnorm @10:07:45.99 AS.seek 0ml @10:08:54.74 -> exit @10:08:54.76 Session Ended @10:09:24.36 -> exit @10:09:24.38 Session Ended @10:23:05.55 Session Begun @10:23:12.28 -> showlog 20 @10:23:57.60 -> ESP.shutdown @10:23:57.64 AS.coast @10:23:57.67 Asleep / Shutdown @10:23:57.75 SS.coast @10:23:57.80 CC.coast @10:23:57.86 CS.coast @10:23:57.92 PC.coast @10:23:57.98 Forearm.coast @10:23:58.62 Elbow.coast @10:23:58.68 SC.coast @10:23:58.74 SE.coast @10:23:59.15 Gate.power :core,:OFF @10:23:59.17 Gate.power :raw,:OFF @10:23:59.20 Shutdown @10:24:07.99 -> exit @10:24:08.00 Session Ended @10:52:18.42 Session Begun @10:52:27.34 -> ESP.wakeup @10:52:27.41 Powering core bus @10:52:32.38 Gate.power :raw,:ON @10:52:33.09 SPE.coast @10:52:33.18 SPE.configure SPEconfig @10:52:33.30 AS.configure ASconfig @10:52:33.43 SS.configure SSconfig @10:52:33.55 CC.configure CCconfig @10:52:33.68 CS.configure CSconfig @10:52:33.80 PC.configure PCconfig @10:52:33.92 PS.configure PSconfig @10:52:34.07 Forearm.configure LooseForearm @10:52:34.18 Elbow.configure LooseElbow @10:52:34.33 SC.configure SCloose @10:52:34.45 SE.configure SEconfig @10:52:34.52 CH.coast @10:52:34.60 CH.configure CHconfig @10:52:34.66 PH.coast @10:52:34.76 PH.configure PHconfig @10:52:34.83 PCR.off @10:52:34.88 PCR.pidtemp= 50,0.1,0,300,25,25 @10:52:34.92 PCR.caltemp= 2.7073,0.0373,0 @10:52:34.97 All dwarf controllers running firmware version 3.75 @10:52:35.11 Valve.reset @10:52:35.30 PCR.off @10:52:35.35 PCR.pidtemp= 50,0.1,0,300,25,25 @10:52:35.40 PCR.caltemp= 2.7073,0.0373,0 @10:52:35.62 Intake.select :close @10:52:35.68 PSV.dial :air @10:52:35.71 Exhaust.select :close @10:52:35.76 CTV.dial :air @10:52:35.91 CSV.dial :waste1 @10:52:35.96 PS.reconfigure PSconfig @10:52:36.12 PS.home.seek 0ml @10:52:36.16 SSV.dial :spit,avoiding: :exhaust @10:52:36.20 Forearm.reconfigure LooseForearm @10:52:36.39 Forearm.home.seek :retract @10:52:36.53 CS.reconfigure CSfast @10:52:36.56 SS.reconfigure SSconfig @10:52:36.67 CS.home.seek 0ml @10:52:36.71 SS.home.seek 0ml @10:52:37.20 Hand.close @10:52:37.23 Elbow.jog 1500 @10:52:37.81 Elbow.reconfigure LooseElbow @10:52:37.88 Elbow.home.seek :clear @10:52:42.59 SE.reconfigure SEconfig @10:52:42.67 SE.home.seek :down @10:52:43.41 SC.reconfigure SCsloppy @10:52:43.48 SC.home.seek :initial @10:52:45.39 PS.coast @10:52:47.59 Forearm.seek :retract @10:52:47.70 Elbow.seek :hammer @10:52:48.89 Forearm.reconfigure TightForearm @10:52:48.97 Forearm.seek 5 + 350 counts @10:52:50.04 Forearm.seek :retract @10:52:51.33 PC.reconfigure PCconfig @10:52:51.35 CC.reconfigure CCconfig @10:52:51.47 PC.home.seek :open @10:52:51.50 CC.home.seek :open @10:55:00.50 -> abort! "will run a coreNeg and increase sampling volume" @10:55:00.52 CmdServer::Client::Error in mwj -- Aborting Mission: will run a coreNeg and increase sampling volume @10:55:00.60 BadNews.email "will run a coreNeg and increase sampling volume",Subject: "18tundeployv3 ABORTED!" @10:55:14.01 Session Ended @11:04:00.01CEST18-Oct-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @11:04:01.94 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @11:04:02.24 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @11:04:07.12 Polling Can every 10:00 @11:04:08.94 SPE.coast @11:04:09.01 SPE.configure SPEconfig @11:04:09.10 AS.configure ASconfig @11:04:09.20 SS.reconfigure SSconfig @11:04:09.24 SS.hold @11:04:09.31 SS.configure SSconfig @11:04:09.41 CC.reconfigure CCconfig @11:04:09.45 CC.hold @11:04:09.54 CC.configure CCconfig @11:04:09.64 CS.reconfigure CSconfig @11:04:09.68 CS.hold @11:04:09.75 CS.configure CSconfig @11:04:09.85 PC.reconfigure PCconfig @11:04:09.89 PC.hold @11:04:10.33 PC.configure PCconfig @11:04:10.42 PS.configure PSconfig @11:04:10.54 Forearm.reconfigure LooseForearm @11:04:10.58 Forearm.hold @11:04:10.63 Forearm.configure LooseForearm @11:04:10.72 Elbow.reconfigure LooseElbow @11:04:10.77 Elbow.hold @11:04:10.82 Elbow.configure LooseElbow @11:04:10.94 SC.reconfigure SCloose @11:04:10.99 SC.hold @11:04:11.05 SC.configure SCloose @11:04:11.15 SE.reconfigure SEconfig @11:04:11.20 SE.hold @11:04:11.25 SE.configure SEconfig @11:04:11.30 CH.coast @11:04:11.35 CH.configure CHconfig @11:04:11.38 PH.coast @11:04:11.44 PH.configure PHconfig @11:04:11.48 PCR.off @11:04:11.51 PCR.pidtemp= 50,0.1,0,300,25,25 @11:04:11.54 PCR.caltemp= 2.7073,0.0373,0 @11:04:11.57 All dwarf controllers running firmware version 3.75 @11:04:11.81 Cleanly exited at 10:55:13CEST18-Oct-18 @11:04:11.84 Loading /home/esp/esp2/mission/18tundeployv4.rb mission @11:04:11.92 Preparing to sleep until 14:00 18-oct-18 @11:04:11.95 GoodNews.email "Starting 18tundeployv4 w/pucks in tube 3\n{1 => 22, 2 => 20, 3 => 2}\nCan@11:04:07, 13.8C, 71% humidity, 14.3psia, 12.871V, 0.859A, 0.865A avg, 11.06W",Subject: "18tundeployv4 begun" @11:04:12.02 SS.coast @11:04:12.05
Asleep til 14:00 18-oct-18 @11:04:12.10 CC.coast @11:04:12.16
Continuing at 14:00 @11:04:12.24 CS.coast @11:04:12.32 PC.coast @11:04:12.36 Forearm.coast @11:04:12.40 Elbow.coast @11:04:12.43 SC.coast @11:04:12.46 SE.coast @11:04:12.86 Gate.power :core,:OFF @11:04:12.87 Gate.power :raw,:OFF @11:04:12.88 Shutdown @11:04:29.36 GoodNews.email "Then, in tube 3: coreNEG\n/home/esp/esp2/mission/18tundeployv4.rb:118\n{1 => 22, 2 => 20, 3 => 2}\nCan@11:04:12, 13.8C, 71% humidity, 14.3psia, 12.871V, 0.878A, 0.878A avg, 11.30W",Subject: "Asleep until 14:00" @14:00:00.05
Powering core bus @14:00:04.99 Gate.power :raw,:ON @14:00:05.64 SPE.coast @14:00:05.70 SPE.configure SPEconfig @14:00:05.79 AS.configure ASconfig @14:00:05.89 SS.configure SSconfig @14:00:06.00 CC.configure CCconfig @14:00:06.09 CS.configure CSconfig @14:00:06.19 PC.configure PCconfig @14:00:06.29 PS.configure PSconfig @14:00:06.42 Forearm.configure LooseForearm @14:00:06.51 Elbow.configure LooseElbow @14:00:06.63 SC.configure SCloose @14:00:06.74 SE.configure SEconfig @14:00:06.79 CH.coast @14:00:06.84 CH.configure CHconfig @14:00:06.87 PH.coast @14:00:06.94 PH.configure PHconfig @14:00:06.98 PCR.off @14:00:07.01 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:07.07 PCR.caltemp= 2.7073,0.0373,0 @14:00:07.13 All dwarf controllers running firmware version 3.75 @14:00:07.27 Valve.reset @14:00:07.44 PCR.off @14:00:07.47 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:07.49 PCR.caltemp= 2.7073,0.0373,0 @14:00:07.63 Intake.select :close @14:00:07.70 Exhaust.select :close @14:00:07.74 CTV.dial :air @14:00:08.24 PSV.dial :air @14:00:08.27 CSV.dial :waste1 @14:00:08.53 SSV.dial :spit,avoiding: :exhaust @14:00:08.58 PS.reconfigure PSconfig @14:00:08.61
Forearm.reconfigure LooseForearm @14:00:08.77 PS.home.seek 0ml @14:00:08.79
Forearm.home.seek :retract @14:00:08.98 CS.reconfigure CSfast @14:00:09.01 SS.reconfigure SSconfig @14:00:09.11 CS.home.seek 0ml @14:00:09.14 SS.home.seek 0ml @14:00:09.60 Hand.close @14:00:09.63
Elbow.jog 1500 @14:00:10.19 Elbow.reconfigure LooseElbow @14:00:10.24 Elbow.home.seek :clear @14:00:14.95 SE.reconfigure SEconfig @14:00:15.00 SE.home.seek :down @14:00:15.72 SC.reconfigure SCsloppy @14:00:15.77 SC.home.seek :initial @14:00:17.85 PS.coast @14:00:19.79
Forearm.seek :retract @14:00:19.87 Elbow.seek :hammer @14:00:21.21 Forearm.reconfigure TightForearm @14:00:21.26 Forearm.seek 5 + 350 counts @14:00:22.33 Forearm.seek :retract @14:00:23.62 PC.reconfigure PCconfig @14:00:23.63
CC.reconfigure CCconfig @14:00:23.73 PC.home.seek :open @14:00:23.76
CC.home.seek :open @14:00:27.17 Phase [coreNEG] @14:00:27.18 GoodNews.email "At 14:00:27 began in tube 3: coreNEG\n/home/esp/esp2/mission/18tundeployv4.rb:118\n{1 => 22, 2 => 20, 3 => 2}\nCan@14:00:27, 13.5C, 71% humidity, 14.3psia, 12.930V, 0.802A, 0.802A avg, 10.37W",Subject: "Started 18tundeployv4 phase coreNEG" @14:00:27.20CEST18-Oct-18
==================> STARTED coreNEG (phase #1) <=== @14:00:27.22 Starting initialPurge @14:01:07.36 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:01:07.38 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:01:07.41 Retry #1 of 10 after 10:00 @14:01:07.50
Hand.open @14:01:08.20 Elbow.reconfigure TightElbow @14:01:08.25 Elbow.seek FlushPuck::Garage @14:01:10.12 Elbow.reconfigure LooseElbow @14:01:10.22 Forearm.reconfigure LooseForearm @14:01:10.27 Forearm.seek :garage @14:01:11.23 Hand.close @14:01:11.95 Forearm.seek :retract @14:01:12.94 Elbow.reconfigure TightElbow @14:01:12.99 Elbow.seek CC @14:01:15.95 Elbow.reconfigure LooseElbow @14:01:16.05 Forearm.reconfigure TightForearm @14:01:16.10 Forearm.seek CC @14:01:17.39 CC.seek :closed @14:01:21.64 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.15 @14:01:21.72 Hand.open @14:01:22.38 Forearm.reconfigure LooseForearm @14:01:22.43 Forearm.seek :retract @14:01:23.46 Flushing the Collection Valve::Manifold @14:01:23.59 CSV.dial CSR @14:01:25.06 Pulling 5ml of CSR.flush @14:01:25.07 CSR.select :flush @14:01:25.19 CS.reconfigure CSconfig @14:01:25.23 CS.seek 5ml @14:01:39.06 delay 5 seconds @14:01:44.08 CSR.select :air @14:01:44.25 CS.reconfigure CSfast @14:01:44.30 CS.seek 7.5ml @14:01:51.30 CSV.dial :waste @14:01:52.69 CS.reconfigure CSconfig @14:01:52.74 CS.seek 0ml @14:02:12.98 Purging external Intake @14:02:13.12 CSV.dial :flush @14:02:13.61 CS.reconfigure CSfast @14:02:13.66 CS.seek 10ml @14:02:38.59 delay 5 seconds @14:02:43.61 CSV.dial CBV @14:02:43.64
CBV.dial CSV @14:02:44.46 CTV.dial :intake @14:02:45.28 Intake.select :open @14:02:45.40 CS.seek 0ml @14:03:11.40 Intake.select :close @14:03:11.51 CTV.dial :air @14:03:12.31 Flushing upper manifold @14:03:12.44 CSV.dial :flush @14:03:13.23 CS.seek 5ml @14:03:26.22 delay 5 seconds @14:03:31.25 CSV.dial :air @14:03:31.80 CS.seek 7ml @14:03:37.56 CSV.dial CBV @14:03:37.58
CBV.dial CSV @14:03:38.70 CTV.dial :air @14:03:38.72 CS.seek 0ml @14:03:56.50 delay 7 seconds @14:04:03.53 CS.seek 3ml @14:04:11.74 CSV.dial :waste @14:04:12.23 CS.seek 0ml @14:04:20.52 CSV.dial :flush @14:04:21.00 CS.seek 3ml @14:04:29.12 delay 5 seconds @14:04:34.14 CSV.dial :air @14:04:34.70 CS.seek 5ml @14:04:40.55 CSV.dial :air @14:04:40.58 CS.seek 0ml @14:04:53.53 delay 7 seconds @14:05:00.56 CS.seek 3ml @14:05:08.80 CSV.dial :waste @14:05:09.59 CS.seek 0ml @14:05:17.81 Garaging FlushPuck in Collection Clamp @14:05:17.89 Elbow.reconfigure TightElbow @14:05:17.94 Elbow.seek CC @14:05:18.20 Elbow.reconfigure LooseElbow @14:05:18.30 Forearm.seek CC @14:05:19.29 Hand.close @14:05:20.47 CC.seek :open @14:05:24.91 Forearm.seek :retract @14:05:25.92 Elbow.reconfigure TightElbow @14:05:25.97 Elbow.seek :garage @14:05:29.10 Elbow.reconfigure LooseElbow @14:05:29.28 Forearm.reconfigure TightForearm @14:05:29.32 Forearm.seek :garage @14:05:30.46 Hand.open @14:05:31.27 Forearm.reconfigure LooseForearm @14:05:31.32 Forearm.seek :retract @14:05:32.43 Starting mfb @14:05:32.44 pcrBAC wcr sampling at most 1500ml @14:05:32.50 Loading sh1 puck from tube 3 into Collection Clamp @14:05:32.62 Readying the MFB @14:05:32.67 Homing MFB Syringe @14:05:32.70 AFV.select :holdingCoil @14:05:32.94 ATV.dial :air2 @14:05:32.97
SC.reconfigure SCtight @14:05:33.06 SC.seek 3 @14:05:34.50 AS.reconfigure ASconfig @14:05:34.55 AS.home.seek :home @14:05:35.13
SC.reconfigure SCloose @14:05:35.36 Elbow.reconfigure TightElbow @14:05:35.41 Elbow.seek 3 @14:05:37.48 Forearm.reconfigure TightForearm @14:05:37.53 Forearm.seek 3 + 700 counts @14:05:38.63 SE.reconfigure SEpick @14:05:38.70 SE.seek beamBreak @14:05:40.08 AFV.select :carrierSolution @14:05:40.20 delay 2 seconds @14:05:41.65
SE.configure SEconfig @14:05:41.83 Elbow.seek 3 @14:05:42.12 Forearm.seek 3 + 300 counts @14:05:42.22 AS.seek 1ml @14:05:42.31
Forearm.reconfigure LooseForearm @14:05:42.36 Hand.close @14:05:42.97 SE.seek :down @14:05:43.20
Forearm.seek :retract @14:05:44.14 Elbow.seek CC @14:05:45.83 SE.coast @14:05:45.87
Elbow.reconfigure LooseElbow @14:05:45.99 Forearm.reconfigure TightForearm @14:05:46.04 Forearm.seek CC @14:05:47.33 CC.seek :closed @14:05:51.59 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.22 @14:05:51.60 Hand.open @14:05:52.28 Forearm.reconfigure LooseForearm @14:05:52.33 Forearm.seek :retract @14:05:53.29 SC.coast @14:05:53.34 skipping prime and sample collection @14:05:53.35 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @14:05:53.36 Intake.select :close @14:05:53.44 CSV.dial :waste @14:05:53.48 CS.seek 0ml @14:05:53.65 CSV.dial :air @14:05:53.69
Calibrating pressure and temperature sensors @14:05:53.73 CTV.dial :air @14:05:53.75 SSV.dial :spit,avoiding: :exhaust @14:05:53.78 SS.reconfigure SSfast @14:05:53.83 SS.seek 5ml @14:05:54.51 CS.seek 10ml @14:05:56.73 SSV.dial CBV @14:05:56.75
CBV.dial SSV @14:05:58.73 delay 3.8 seconds @14:06:02.68 SSV.dial :spit @14:06:04.10 SS.seek 0ml @14:06:06.58 SSV.dial CBV @14:06:07.98 SSV.dial CBV,avoiding: :exhaust @14:06:08.01
CBV.dial SSV @14:06:08.02 CTV.dial :air @14:06:08.04 delay 15 seconds @14:06:23.07 SSV.dial :spit,avoiding: :exhaust @14:06:24.50 CTV.dial CSV,avoiding: :intake @14:06:24.55
SS.reconfigure SSexhale @14:06:24.64 SS.seek 0ml @14:06:24.78 SSV.dial CBV,avoiding: :exhaust @14:06:26.29 CBV.dial between SSV and waste2 @14:06:26.70 CTV.dial CSV,avoiding: :air @14:06:26.73
CSV.dial CTV @14:06:27.20 CS.reconfigure CSpressurize @14:06:27.25 CS.seek 0ml @14:06:35.14 delay 1 second @14:06:36.15 AFV.select :holdingCoil @14:06:36.26 delay 1 second @14:06:37.28 ATV.dial :waste @14:06:38.39 AS.reconfigure ASnorm @14:06:38.44 AS.home.seek :empty @14:06:53.46
CSV.dial :air @14:06:53.95 CS.reconfigure CSfast @14:06:54.00 CS.seek 10ml @14:07:18.72 CSV.dial between air and CTV @14:07:19.07 CS.seek 3.428ml @14:07:31.00 ATV.dial :waste @14:07:31.02 ARV.dial between carrier and air1 @14:07:31.11 ASV.dial :waste1 @14:07:35.66 CTV.dial CSV,avoiding: :air @14:07:35.69
CSV.dial CTV @14:07:36.04 CS.reconfigure CSpressurize @14:07:36.08 CS.seek 0ml @14:07:45.49 CSV.dial :air @14:07:45.99 CS.reconfigure CSfast @14:07:46.04 CS.seek 10ml @14:08:10.72 CSV.dial between air and CTV @14:08:11.08 CS.seek 2.162ml @14:08:30.66 CTV.dial CSV,avoiding: :air @14:08:30.69
CSV.dial CTV @14:08:31.02 CS.reconfigure CSpressurize @14:08:31.07 CS.seek 0ml @14:08:33.40 BubblePoint: Intake @69.6psia, Outlet @14.3psia CBV.dial SSV @14:08:33.75 SS.reconfigure SSconfig @14:08:33.80 SS.seek 10ml @14:08:39.86 delay 15 seconds @14:08:55.00 Evacuated: Intake @28.3psia, Outlet @28.5psia @14:08:55.01 SSV.dial :spit,avoiding: :exhaust @14:08:56.44 SS.reconfigure SSexhale @14:08:56.49 SS.seek 0ml @14:09:01.35 SS.reconfigure SSfast @14:09:01.40 SS.seek 5ml @14:09:04.03 SSV.dial CBV,avoiding: :exhaust @14:09:04.06
CBV.dial SSV @14:09:05.48 CTV.dial :air,avoiding: :intake @14:09:06.89 CSV.dial CTV @14:09:06.94
SS.seek 10ml @14:09:07.93 CSV.dial :air @14:09:08.43 CS.reconfigure CSfast @14:09:08.48 CS.seek 0ml @14:09:09.64
SSV.dial :spit,avoiding: :exhaust @14:09:11.08 SS.reconfigure SSexhale @14:09:11.13 SS.seek 0ml @14:09:12.96 CS.coast @14:09:16.23
SS.reconfigure SSfast @14:09:16.28 SS.seek 5ml @14:09:18.98 SSV.dial CBV,avoiding: :exhaust @14:09:19.01
CBV.dial SSV @14:09:21.93 SSV.dial :spit,avoiding: :exhaust @14:09:23.34 SS.reconfigure SSconfig @14:09:23.39 SS.seek 0ml @14:09:26.71 SS.coast @14:09:26.76 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @14:09:26.77 Intake.select :close @14:09:26.85 CSV.dial :waste @14:09:27.00 SSV.dial CBV,avoiding: :exhaust @14:09:27.03
CBV.dial SSV @14:09:27.69 CS.seek 0ml @14:09:27.76 CSV.dial :air @14:09:28.47
CTV.dial :air @14:09:28.48 delay 15 seconds @14:09:28.55 CS.seek 10ml @14:09:43.51
SSV.dial :spit,avoiding: :exhaust @14:09:44.94 CTV.dial CSV,avoiding: :intake @14:09:44.98
SS.reconfigure SSexhale @14:09:45.08 SS.seek 0ml @14:09:45.15 SSV.dial CBV,avoiding: :exhaust @14:09:53.19 CBV.dial between SSV and waste2 @14:09:53.64 CTV.dial CSV,avoiding: :air @14:09:53.67
CSV.dial CTV @14:09:54.16 CS.reconfigure CSpressurize @14:09:54.21 CS.seek 0ml @14:10:20.46 CSV.dial :air @14:10:20.99 CS.reconfigure CSfast @14:10:21.04 CS.seek 10ml @14:10:45.85 CSV.dial between air and CTV @14:10:46.20 CS.seek 3.405ml @14:11:02.85 CTV.dial CSV,avoiding: :air @14:11:02.88
CSV.dial CTV @14:11:03.21 CS.reconfigure CSpressurize @14:11:03.26 CS.seek 0ml @14:11:07.44 GoodNews.email "At 14:00:27 began in tube 3: coreNEG\n/home/esp/esp2/mission/18tundeployv4.rb:118\n{1 => 22, 2 => 20, 3 => 2}\nCan@14:00:27, 13.5C, 71% humidity, 14.3psia, 12.930V, 0.802A, 0.802A avg, 10.37W",Subject: "Started 18tundeployv4 phase coreNEG" @14:11:47.55 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:11:47.59
CSV.dial :air @14:11:47.61 Retry #2 of 10 after 10:00 @14:11:48.13
CS.reconfigure CSfast @14:11:48.18 CS.seek 10ml @14:12:12.91 CSV.dial between air and CTV @14:12:13.27 CS.seek 2.151ml @14:12:32.81 CTV.dial CSV,avoiding: :air @14:12:32.84
CSV.dial CTV @14:12:33.16 CS.reconfigure CSpressurize @14:12:33.23 CS.seek 0ml @14:12:37.19 BubblePoint: Intake @69.6psia, Outlet @14.8psia @14:12:37.33 CBV.dial SSV @14:12:38.08 SS.reconfigure SSconfig @14:12:38.70 SS.seek 10ml @14:12:45.08 delay 15 seconds @14:13:00.30 Evacuated: Intake @28.2psia, Outlet @28.4psia @14:13:00.31 SSV.dial :spit,avoiding: :exhaust @14:13:01.75 SS.reconfigure SSexhale @14:13:01.80 SS.seek 0ml @14:13:06.93 SS.reconfigure SSfast @14:13:06.98 SS.seek 5ml @14:13:09.44 SSV.dial CBV,avoiding: :exhaust @14:13:09.46
CBV.dial SSV @14:13:10.89 CTV.dial :air,avoiding: :intake @14:13:12.30 CSV.dial CTV @14:13:12.35
SS.seek 10ml @14:13:13.34 CSV.dial :air @14:13:13.84 CS.reconfigure CSfast @14:13:13.89 CS.seek 0ml @14:13:15.22
SSV.dial :spit,avoiding: :exhaust @14:13:16.66 SS.reconfigure SSexhale @14:13:16.71 SS.seek 0ml @14:13:18.34 CS.coast @14:13:21.85
SS.reconfigure SSfast @14:13:21.90 SS.seek 5ml @14:13:24.62 SSV.dial CBV,avoiding: :exhaust @14:13:24.65
CBV.dial SSV @14:13:27.59 SSV.dial :spit,avoiding: :exhaust @14:13:29.01 SS.reconfigure SSconfig @14:13:29.06 SS.seek 0ml @14:13:32.04 SS.coast @14:13:32.09 Lysing: Adding 1.5ml lysis @14:13:32.10 CBV.dial between CSV and waste1,avoiding: :DBV @14:13:33.71 CTV.dial CSV @14:13:35.13 CSV.dial CSR @14:13:35.97 Pulling 1ml of CSR.lysis @14:13:35.98 CSR.select :lysis @14:13:36.10 CS.reconfigure CSconfig @14:13:36.15 CS.seek 1ml @14:13:39.70 delay 5 seconds @14:13:44.72 CSR.select :air @14:13:44.90 CS.seek 6ml @14:13:58.73 delay 2 seconds @14:14:00.74 CSV.dial :waste1 @14:14:02.15 CS.reconfigure CSfast @14:14:02.20 CS.seek :empty @14:14:17.31 CSV.dial CSR @14:14:18.76 Pulling 1.5ml of CSR.lysis @14:14:18.77 CSR.select :lysis @14:14:18.89 CS.reconfigure CSconfig @14:14:18.94 CS.seek 1.5ml @14:14:23.76 delay 5 seconds @14:14:28.78 CSR.select :air @14:14:28.97 CS.seek 4.5ml @14:14:37.67 delay 2 seconds @14:14:39.68 CSV.dial CTV @14:14:40.19 CS.seek 1.275ml @14:14:49.36 Heating sh1 puck from tube 3 in Collection Clamp to 85C for 5:00 @14:14:49.38 CH.seek 85C,8:00 @14:17:56.82 CH.hold 5:00,85C @14:21:47.65 GoodNews.email "At 14:00:27 began in tube 3: coreNEG\n/home/esp/esp2/mission/18tundeployv4.rb:118\n{1 => 22, 2 => 20, 3 => 2}\nCan@14:00:27, 13.5C, 71% humidity, 14.3psia, 12.930V, 0.802A, 0.802A avg, 10.37W",Subject: "Started 18tundeployv4 phase coreNEG" @14:21:59.85 BACfans.email "Started mfb in tube 3 at 14:05:32CEST18-Oct-18\n{1 => 22, 2 => 20, 3 => 2}\nCan@14:04:10, 13.5C, 71% humidity, 14.3psia, 12.910V, 0.821A, 0.815A avg, 10.60W",Subject: "pcrBAC wcr sampling at most 1500ml" @14:22:57.00
Collection Puck Heater at 85C after lysing @14:22:57.02 CS.seek 0ml @14:23:01.20 CTV.dial between spare and air,avoiding: :intake @14:23:02.33 CSV.dial :air @14:23:02.82 CS.seek 5ml @14:23:16.63 CSV.dial CTV @14:23:17.13 delay 2 seconds @14:23:19.14 CSV.dial :air @14:23:19.64 delay 2 seconds @14:23:21.66 CSV.dial CTV @14:23:22.16 delay 2 seconds @14:23:24.17 CSV.dial :air @14:23:24.67 delay 2 seconds @14:23:26.69 CS.seek 0ml @14:23:40.86 Recovering Lysate into Collection syringe @14:23:40.87 CSV.dial CBV @14:23:41.97 CBV.dial CSV @14:23:42.37 CS.seek 4.999ml @14:23:56.11 delay 5 seconds @14:24:01.13 CTV.dial :air @14:24:01.71 CS.seek 6.999ml @14:24:07.80 delay 5 seconds @14:24:12.82 CSV.dial :air2 @14:24:13.28 CS.seek 10ml @14:24:22.08 CSV.dial between air and CTV,avoiding: CBV @14:24:23.33 Lysing Complete @14:24:23.41 Storing sh1 puck in Collection Clamp to tube 2 @14:24:23.60 SC.reconfigure SCtight @14:24:23.65 SC.seek 2 @14:24:25.78 SC.reconfigure SCloose @14:24:26.01 Elbow.reconfigure TightElbow @14:24:26.06 Elbow.seek 2 @14:24:27.93 Forearm.reconfigure TightForearm @14:24:27.98 Forearm.seek 2 + 700 counts @14:24:29.02 SE.reconfigure SEpick @14:24:29.09 SE.seek beamBreak @14:24:30.35 SE.configure SEconfig @14:24:30.53 Elbow.reconfigure LimpElbow @14:24:30.58 Forearm.reconfigure LooseForearm @14:24:30.63 Forearm.seek 2 @14:24:30.94 Elbow.hold @14:24:31.01 Elbow.reconfigure TightElbow @14:24:31.12 SE.seek 19.1 Pucks below Top Plate @14:24:31.70 Forearm.force -20 @14:24:32.23 Forearm.seek 2 + 339 counts @14:24:32.57 Forearm.reconfigure TightForearm @14:24:32.62 Forearm.seek 2 + 700 counts @14:24:32.88 SE.seek 20.1 Pucks below Top Plate @14:24:33.58 Forearm.reconfigure LooseForearm @14:24:33.63 Forearm.seek :retract @14:24:34.56 Elbow.seek CC @14:24:36.29 Elbow.reconfigure LooseElbow @14:24:36.39 Forearm.seek CC @14:24:37.36 Hand.close @14:24:38.11 CC.seek :open @14:24:42.62 Forearm.seek :retract @14:24:43.61 Elbow.reconfigure TightElbow @14:24:43.66 Elbow.seek 2 @14:24:45.48 Elbow.reconfigure LimpElbow @14:24:45.53 Forearm.seek 2 @14:24:46.56 Elbow.hold @14:24:46.64 Elbow.reconfigure TightElbow @14:24:46.68 Hand.open @14:24:47.49 Forearm.force -20 @14:24:47.54 Forearm.seek 2 + 348 counts @14:24:47.87 Forearm.reconfigure TightForearm @14:24:47.92 Forearm.seek 2 + 700 counts @14:24:48.27 SE.seek :down @14:24:49.12 SE.coast @14:24:49.43
Forearm.reconfigure LooseForearm @14:24:49.48 Forearm.seek :retract @14:24:50.40 SC.coast @14:24:50.44 Tranferring lysate to PRV @14:24:50.45 CSV.dial :air @14:24:50.78 CS.seek 10ml @14:24:50.86 CSV.dial between air and CTV @14:24:51.21 AIV.dial :bypass @14:24:51.30 Beginning samplePassC @14:24:51.31 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:24:51.40 Hand.open @14:24:51.75 Elbow.seek FlushPuck::Garage @14:24:53.73 Elbow.reconfigure LooseElbow @14:24:53.84 Forearm.seek :garage @14:24:54.82 Hand.close @14:24:55.61 Forearm.seek :retract @14:24:56.62 Elbow.reconfigure TightElbow @14:24:56.67 Elbow.seek CC @14:24:59.61 Elbow.reconfigure LooseElbow @14:24:59.72 Forearm.reconfigure TightForearm @14:24:59.77 Forearm.seek CC @14:25:01.56 CC.seek :closed @14:25:05.83 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.15 @14:25:05.91 Hand.open @14:25:06.55 Forearm.reconfigure LooseForearm @14:25:06.60 Forearm.seek :retract @14:25:07.58 AWV.select :air @14:25:07.60 AIV.dial :bypass @14:25:07.61 PSV.dial PSR @14:25:09.08 Pulling 1.5ml of PSR.flush @14:25:09.09 PSR.select :flush @14:25:09.22 PS.seek 1.5ml @14:25:25.22 delay 5 seconds @14:25:30.24 PSV.dial :waste1 @14:25:30.75 PS.seek 0ml @14:25:46.84 PSV.dial PSR @14:25:47.34 PSR.select :air @14:25:47.48 PS.seek 2.5ml @14:26:13.63 PRV.dial CBV @14:26:13.66
CBV.dial PRV @14:26:14.56 PSV.dial :mixloop @14:26:15.67 CTV.dial :air,avoiding: :intake @14:26:15.68 PTV.dial :PRVmixing @14:26:15.77 PS.seek 0ml @14:26:41.94 PSV.dial :air @14:26:42.43 PS.seek 2.5ml @14:27:08.49 PSV.dial :mixloop @14:27:08.98 PS.seek 0ml @14:27:35.06 PSV.dial :air @14:27:35.56 PS.seek 2.5ml @14:28:01.77 PSV.dial :mixloop @14:28:02.25 PS.seek 0ml @14:28:28.50 Garaging FlushPuck in Collection Clamp @14:28:28.58 Elbow.reconfigure TightElbow @14:28:28.63 Elbow.seek CC @14:28:28.88 Elbow.reconfigure LooseElbow @14:28:28.99 Forearm.seek CC @14:28:29.96 Hand.close @14:28:30.61 CC.seek :open @14:28:35.03 Forearm.seek :retract @14:28:36.02 Elbow.reconfigure TightElbow @14:28:36.07 Elbow.seek :garage @14:28:39.06 Elbow.reconfigure LooseElbow @14:28:39.17 Forearm.reconfigure TightForearm @14:28:39.22 Forearm.seek :garage @14:28:40.40 Hand.open @14:28:41.06 Forearm.reconfigure LooseForearm @14:28:41.11 Forearm.seek :retract @14:28:42.14 Connecting PS to CS to transfer sample @14:28:42.15 CBV.dial AIV @14:28:42.16 AIV.dial :bypass @14:28:42.17 PSV.dial :mixloop @14:28:42.19 PTV.dial :PRVmixing @14:28:42.20 PRV.dial AIV @14:28:42.21 CTV.dial :air,avoiding: :intake @14:28:42.23 Loading lyfil puck from tube 3 into Collection Clamp @14:28:42.49 SC.reconfigure SCtight @14:28:42.54 SC.seek 3 @14:28:44.69 SC.reconfigure SCloose @14:28:45.44 Elbow.reconfigure TightElbow @14:28:45.49 Elbow.seek 3 @14:28:47.65 Forearm.reconfigure TightForearm @14:28:47.70 Forearm.seek 3 + 700 counts @14:28:48.79 SE.reconfigure SEpick @14:28:48.87 SE.seek beamBreak @14:28:51.86 SE.configure SEconfig @14:28:52.03 Elbow.seek 3 @14:28:52.25 Forearm.seek 3 + 300 counts @14:28:52.44 Forearm.reconfigure LooseForearm @14:28:52.49 Hand.close @14:28:53.13 SE.seek :down @14:28:53.36
Forearm.seek :retract @14:28:54.32 Elbow.seek CC @14:28:56.00 Elbow.reconfigure LooseElbow @14:28:56.06 SE.coast @14:28:56.19
Forearm.reconfigure TightForearm @14:28:56.24 Forearm.seek CC @14:28:57.50 CC.seek :closed @14:29:01.77 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.18 @14:29:01.78 Hand.open @14:29:02.44 Forearm.reconfigure LooseForearm @14:29:02.49 Forearm.seek :retract @14:29:03.48 SC.coast @14:29:03.52 CTV.dial CSV,avoiding: :intake @14:29:04.96 CSV.dial CTV @14:29:05.28 CS.seek 5ml @14:29:19.21 delay 1:20 @14:30:39.24 CS.seek 2.5ml @14:30:46.84 delay 40 seconds @14:31:26.87 CS.seek 0ml @14:31:34.31 delay 1:00 @14:32:34.33 PSV.dial :air @14:32:34.83 PS.seek 2.5ml @14:33:01.00 PSV.dial :mixloop @14:33:01.49 delay 3 seconds @14:33:04.51 PSV.dial :air @14:33:04.99 delay 2 seconds @14:33:07.00 PSV.dial :mixloop @14:33:07.49 delay 3 seconds @14:33:10.51 PSV.dial :waste1 @14:33:11.91 PS.seek :empty @14:33:38.09 CBV.dial :waste1 @14:33:38.60 delay 5 seconds @14:33:43.62 CBV.dial AIV @14:33:44.14 delay 10 seconds @14:33:54.16 CBV.dial :waste1 @14:33:54.68 delay 10 seconds @14:34:04.70 CBV.dial AIV @14:34:05.21 delay 10 seconds @14:34:15.24 CTV.dial CSV,avoiding: :intake @14:34:15.25 CBV.dial between AIV and waste1 @14:34:15.60 CSV.dial :air @14:34:16.09 CS.seek 10ml @14:34:42.55 CSV.dial CTV @14:34:43.04 CS.seek 0ml @14:35:09.77 CBV.dial :waste1 @14:35:10.11 delay 10 seconds @14:35:20.57 CTV.dial :air,avoiding: :intake @14:35:22.14 Storing lyfil puck in Collection Clamp to tube 2 @14:35:22.33 SC.reconfigure SCtight @14:35:22.38 SC.seek 2 @14:35:24.53 SC.reconfigure SCloose @14:35:24.76 Elbow.reconfigure TightElbow @14:35:24.81 Elbow.seek 2 @14:35:26.68 Forearm.reconfigure TightForearm @14:35:26.74 Forearm.seek 2 + 700 counts @14:35:27.76 SE.reconfigure SEpick @14:35:27.83 SE.seek beamBreak @14:35:29.01 SE.configure SEconfig @14:35:29.18 Elbow.reconfigure LimpElbow @14:35:29.24 Forearm.reconfigure LooseForearm @14:35:29.28 Forearm.seek 2 @14:35:29.59 Elbow.hold @14:35:29.66 Elbow.reconfigure TightElbow @14:35:29.77 SE.seek 20.1 Pucks below Top Plate @14:35:30.34 Forearm.force -20 @14:35:30.38 Forearm.seek 2 + 345 counts @14:35:30.72 Forearm.reconfigure TightForearm @14:35:30.77 Forearm.seek 2 + 700 counts @14:35:31.06 SE.seek 21.1 Pucks below Top Plate @14:35:31.76 Forearm.reconfigure LooseForearm @14:35:31.81 Forearm.seek :retract @14:35:32.72 Elbow.seek CC @14:35:34.45 Elbow.reconfigure LooseElbow @14:35:34.56 Forearm.seek CC @14:35:35.50 Hand.close @14:35:36.25 CC.seek :open @14:35:40.76 Forearm.seek :retract @14:35:41.70 Elbow.reconfigure TightElbow @14:35:41.75 Elbow.seek 2 @14:35:43.69 Elbow.reconfigure LimpElbow @14:35:43.74 Forearm.seek 2 @14:35:44.73 Elbow.hold @14:35:44.80 Elbow.reconfigure TightElbow @14:35:44.85 Hand.open @14:35:45.76 Forearm.force -20 @14:35:45.80 Forearm.seek 2 + 346 counts @14:35:46.13 Forearm.reconfigure TightForearm @14:35:46.18 Forearm.seek 2 + 700 counts @14:35:46.52 SE.seek :down @14:35:47.27 SE.coast @14:35:47.67
Forearm.reconfigure LooseForearm @14:35:47.71 Forearm.seek :retract @14:35:48.61 SC.coast @14:35:48.64 PSV.dial :air @14:35:49.80 PS.seek 0.499ml @14:35:55.76 delay 3 seconds @14:35:58.77 PSV.dial :mixloop @14:35:59.76 PRV.dial :air @14:36:00.26 delay 3 seconds @14:36:03.28 PRV.dial CBV @14:36:03.77 CBV.dial AIV @14:36:04.29 PRV.dial between CBV and air @14:36:04.65 PRV.dial :air @14:36:05.05 PS.reconfigure PSslow @14:36:05.10 PS.seek 0.599ml @14:36:07.59 PRV.dial :waste2 @14:36:08.38 PS.reconfigure PSconfig @14:36:08.43 PS.seek :empty @14:36:15.38 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:36:15.45 Hand.open @14:36:15.88 Elbow.seek FlushPuck::Garage @14:36:17.97 Elbow.reconfigure LooseElbow @14:36:18.08 Forearm.seek :garage @14:36:19.11 Hand.close @14:36:19.88 Forearm.seek :retract @14:36:20.89 Elbow.reconfigure TightElbow @14:36:20.94 Elbow.seek CC @14:36:23.81 Elbow.reconfigure LooseElbow @14:36:23.92 Forearm.reconfigure TightForearm @14:36:23.97 Forearm.seek CC @14:36:25.24 CC.seek :closed @14:36:29.48 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.14 @14:36:29.57 Hand.open @14:36:30.32 Forearm.reconfigure LooseForearm @14:36:30.37 Forearm.seek :retract @14:36:31.42 Clearing PML for modified lysate @14:36:31.43 CTV.dial :air,avoiding: :intake @14:36:31.44 PRV.dial :waste2 @14:36:31.46 PTV.dial :PRV,avoiding: :puck @14:36:32.26 delay 2 seconds @14:36:34.29 PTV.dial :PRVmixing,avoiding: :puck @14:36:35.09 delay 2 seconds @14:36:37.10 PSV.dial :PML @14:36:37.12 delay 2 seconds @14:36:39.14 PRV.dial :air @14:36:39.95 delay 2 seconds @14:36:42.04 PS.seek 1.499ml @14:36:58.20 delay 5 seconds @14:37:03.23 PS.seek 2.5ml @14:37:14.16 PSV.dial :air @14:37:14.68 PS.seek 0ml @14:37:40.92 mixing lysate with speDil @14:37:40.93 PSV.dial :PML @14:37:41.42 CBV.dial :PRV @14:37:41.43 PRV.dial :air @14:37:41.44 delay 2 seconds @14:37:43.46 PRV.dial :speDil @14:37:43.96 delay 2 seconds @14:37:46.04 PS.reconfigure PSslow1 @14:37:46.09 PS.seek 0.225ml @14:38:09.57 delay 5 seconds @14:38:14.59 PRV.dial CBV @14:38:15.42 delay 2 seconds @14:38:18.01 PS.seek 0.475ml @14:38:43.64 delay 5 seconds @14:38:48.66 PRV.dial :air @14:38:49.17 delay 2 seconds @14:38:51.20 PS.seek 0.965ml @14:39:41.29 delay 10 seconds @14:39:51.31 PRV.dial CBV @14:39:51.81 CTV.dial :air,avoiding: :intake @14:39:51.82 PSV.dial :air @14:39:52.31 PS.reconfigure PSconfig @14:39:52.36 PS.seek 2.5ml @14:40:08.76 delay 3 seconds @14:40:11.78 PSV.dial PTV @14:40:12.57 PTV.dial :PRV,avoiding: :puck @14:40:13.38 PS.seek 0ml @14:40:39.56 PSV.dial :air @14:40:40.37 PS.seek 2.5ml @14:41:06.57 delay 3 seconds @14:41:09.59 PSV.dial PTV @14:41:10.39 PTV.dial :PRV,avoiding: :puck @14:41:10.41 PS.seek 0ml @14:41:36.64 Pushing modified lysate into AIV Injection Loop @14:41:36.65 CBV.dial AIV @14:41:36.66 AIV.dial :bypass @14:41:36.67 PRV.dial CBV @14:41:36.69 PSV.dial :air @14:41:37.49 PS.seek 2.5ml @14:42:03.64 delay 3 seconds @14:42:06.65 PSV.dial :PML @14:42:07.15 PTV.dial :PRVmixing,avoiding: :puck @14:42:08.03 PS.reconfigure PSslow1 @14:42:08.08 PS.seek 1.74ml @14:43:25.45 delay 5 seconds @14:43:30.47 AIV.dial :sample @14:43:31.10 AWV.select :waste @14:43:31.27 PS.reconfigure PSslow2 @14:43:31.32 PS.seek 1.22ml @14:45:18.98 delay 15 seconds @14:45:34.00 AIV.dial :bypass @14:45:34.61 PS.reconfigure PSconfig @14:45:34.66 PS.seek 0ml @14:45:47.91 PSV.dial :air @14:45:48.40 PS.seek 2.5ml @14:46:14.60 PSV.dial :PML @14:46:15.09 PS.seek 0ml @14:46:41.22 AIV.dial :coilToMFB @14:46:41.24 AWV.select :air @14:46:41.41 Extracting Nucleic Acid from AIV @14:46:41.43
Beginning samplePassD @14:46:41.51 SPE.hold 2:46:40,55C @14:46:41.55 ASV.dial :SPEdelivery @14:46:41.64
AWV.select :air @14:46:41.66 AIV.dial :bypass @14:46:41.67 PSV.dial PSR @14:46:41.98 delay 20 seconds @14:46:42.87
Pulling 1.5ml of PSR.killMFB @14:46:42.88 PSR.select :killMFB @14:46:43.01 PS.seek 1.5ml @14:46:59.08 delay 5 seconds @14:47:02.01 primeLysateINT @14:47:02.02 AWV.select :air @14:47:02.03 AIV.dial :coilToMFB @14:47:02.05 ATV.dial AIV @14:47:03.78 AS.reconfigure ASslow1 @14:47:03.83 AS.seek 0.299ml @14:47:04.09
PSV.dial :waste1 @14:47:04.63 PS.seek 0ml @14:47:20.69 PSV.dial PSR @14:47:21.19 PSR.select :air @14:47:21.33 PS.seek 2.5ml @14:47:47.45 PRV.dial CBV @14:47:47.48
CBV.dial PRV @14:47:47.49 PSV.dial :mixloop @14:47:48.62 CTV.dial :air,avoiding: :intake @14:47:48.63 PTV.dial :PRVmixing @14:47:48.65 PS.seek 0ml @14:48:04.45 delay 10 seconds @14:48:14.47 ATV.dial :air2 @14:48:14.82
PSV.dial PSR @14:48:15.48 AS.seek 0.329ml @14:48:16.54
Pulling 1.5ml of PSR.flush @14:48:16.55 PSR.select :flush @14:48:16.68 PS.seek 1.5ml @14:48:21.80 ATV.dial :waste2 @14:48:22.39 AS.reconfigure ASnorm @14:48:22.44 AS.seek :empty @14:48:32.80
delay 5 seconds @14:48:37.83 PSV.dial :waste1 @14:48:38.34 PS.seek 0ml @14:48:39.45 Lysate primed! @14:48:39.46 AFV.select :holdingCoil @14:48:39.47 ATV.dial :air2 @14:48:40.10 AS.reconfigure ASslow1 @14:48:40.15 AS.seek 0.069ml @14:48:54.42
PSV.dial PSR @14:48:54.62 delay 3 seconds @14:48:55.03
PSR.select :air @14:48:55.16 PS.seek 2.5ml @14:48:57.64 ATV.dial :AIV @14:48:58.49 AS.seek 0.469ml @14:49:21.33 PRV.dial CBV @14:49:21.36
CBV.dial PRV @14:49:21.38 PSV.dial :mixloop @14:49:22.52 CTV.dial :air,avoiding: :intake @14:49:22.53 PTV.dial :PRVmixing @14:49:22.55 PS.seek 0ml @14:49:48.77 PSV.dial PSR @14:49:49.95 Pulling 1.5ml of PSR.flush @14:49:49.96 PSR.select :flush @14:49:50.10 PS.seek 1.5ml @14:50:06.11 delay 5 seconds @14:50:11.13 PSV.dial :waste1 @14:50:11.65 PS.seek 0ml @14:50:19.25 delay 5 seconds @14:50:24.33 Solid Phase Extraction Heater at 47.7C @14:50:24.35 ATV.dial ASV @14:50:24.75 ASV.dial :SPEdelivery @14:50:24.83 AS.seek 0.041ml @14:50:27.73
PSV.dial PSR @14:50:28.24 PSR.select :air @14:50:28.38 PS.seek 2.5ml @14:50:54.58 PRV.dial CBV @14:50:54.61
CBV.dial PRV @14:50:54.62 PSV.dial :mixloop @14:50:55.72 CTV.dial :air,avoiding: :intake @14:50:55.73 PTV.dial :PRVmixing @14:50:55.75 PS.seek 0ml @14:51:21.96 PSV.dial PSR @14:51:23.12 Pulling 1.5ml of PSR.flush @14:51:23.13 PSR.select :flush @14:51:23.26 PS.seek 1.5ml @14:51:39.27 delay 5 seconds @14:51:44.29 PSV.dial :waste1 @14:51:44.80 PS.seek 0ml @14:51:51.20 delay 10 seconds @14:52:00.76
PSV.dial PSR @14:52:01.22 rinseLysateLineINT @14:52:01.23 AFV.select :carrierSolution @14:52:01.34 delay 2 seconds @14:52:01.39
PSR.select :air @14:52:01.52 PS.seek 2.5ml @14:52:03.37 AS.reconfigure ASconfig @14:52:03.42 AS.seek 1ml @14:52:27.61 PRV.dial CBV @14:52:27.64
CBV.dial PRV @14:52:27.65 PSV.dial :mixloop @14:52:28.77 CTV.dial :air,avoiding: :intake @14:52:28.78 PTV.dial :PRVmixing @14:52:28.80 PS.reconfigure PSslow @14:52:28.85 PS.seek 1.2ml @14:52:55.64 PSV.dial :waste1 @14:52:57.07 PS.reconfigure PSconfig @14:52:57.12 PS.seek 0ml @14:52:58.87 delay 1 second @14:52:59.88 AFV.select :holdingCoil @14:52:59.99 delay 1 second @14:53:01.01 ATV.dial AIV @14:53:01.65 AIV.dial :coilToMFB @14:53:01.66 AWV.select :waste @14:53:01.77 AS.reconfigure ASslow0 @14:53:02.34 AS.seek 0ml @14:53:10.07
PSV.dial :air @14:53:11.31 PS.seek 2.5ml @14:53:37.54 PSV.dial :mixloop @14:53:38.03 PS.reconfigure PSslow @14:53:38.09 PS.seek 0ml @14:54:29.24 PSV.dial :air @14:54:29.72 PS.reconfigure PSconfig @14:54:29.77 PS.seek 2.5ml @14:54:43.22 ATV.dial :air2 @14:54:43.90 AS.reconfigure ASslow1 @14:54:43.95 AS.seek 0.05ml @14:54:54.28 ATV.dial :AIV @14:54:55.10 AS.seek 0.01ml @14:54:55.92
PSV.dial :mixloop @14:54:56.41 PS.reconfigure PSslow @14:54:56.47 PS.seek 0ml @14:55:03.42 ATV.dial :waste @14:55:05.00 AS.seek :empty @14:55:07.44 ethanol wash @14:55:07.45 ATV.dial :air2 @14:55:08.69 AS.reconfigure ASslow2 @14:55:08.74 AS.seek 0.009ml @14:55:19.08 delay 3 seconds @14:55:22.11 Pulling 0.4ml of Ethanol Wash @14:55:22.12 ATV.dial :ethanol @14:55:22.91 AS.reconfigure ASslow1 @14:55:22.96 AS.seek 0.409ml @14:55:47.52
Cleaning Collection Syringe @14:55:47.53 CSV.dial CSR @14:55:48.08 Pulling 4ml of CSR.flush @14:55:48.09 CSR.select :flush @14:55:48.22 CS.seek 4ml @14:55:59.48 delay 5 seconds @14:56:04.50 CSR.select :air @14:56:04.67 CS.seek 10ml @14:56:21.02 CSV.dial CTV @14:56:21.05
CTV.dial CSV @14:56:22.55 CBV.dial :waste1 @14:56:23.06 CS.seek 0ml @14:56:43.65 delay 3 seconds @14:56:46.67 ATV.dial :air1 @14:56:47.08 delay 3 seconds @14:56:49.94
CSV.dial CSR @14:56:50.16 AS.seek 0.434ml @14:56:50.50
Pulling 4ml of CSR.flush @14:56:50.51 CSR.select :flush @14:56:50.64 CS.seek 4ml @14:56:55.45 delay 3 seconds @14:56:58.47 ATV.dial :ethanol @14:56:59.08 AS.seek 0.419ml @14:57:01.83
delay 5 seconds @14:57:02.40 delay 3 seconds @14:57:05.42 ATV.dial ASV @14:57:06.71 AS.seek 0.092ml @14:57:06.84
CSR.select :air @14:57:06.98 CS.seek 10ml @14:57:23.24 CSV.dial CTV @14:57:23.27
CTV.dial CSV @14:57:23.80 CBV.dial :waste1 @14:57:23.82 CS.seek 0ml @14:57:50.34 Garaging FlushPuck in Collection Clamp @14:57:50.42 Elbow.reconfigure TightElbow @14:57:50.46 Elbow.seek CC @14:57:50.74 Elbow.reconfigure LooseElbow @14:57:50.84 Forearm.seek CC @14:57:51.83 Hand.close @14:57:52.53 CC.seek :open @14:57:56.90 Forearm.seek :retract @14:57:57.89 Elbow.reconfigure TightElbow @14:57:57.94 Elbow.seek :garage @14:58:01.56 Elbow.reconfigure LooseElbow @14:58:01.67 Forearm.reconfigure TightForearm @14:58:01.72 Forearm.seek :garage @14:58:02.87 Hand.open @14:58:03.60 Forearm.reconfigure LooseForearm @14:58:03.65 Forearm.seek :retract @14:58:04.67 Whole Cell Archive sampling 1500ml @14:58:04.69 Loading wcr puck from tube 4 into Collection Clamp @14:58:04.96 SC.reconfigure SCtight @14:58:05.02 SC.seek 4 @14:58:08.70 SC.reconfigure SCloose @14:58:08.94 Elbow.reconfigure TightElbow @14:58:08.99 Elbow.seek 4 @14:58:11.03 Forearm.reconfigure TightForearm @14:58:11.09 Forearm.seek 4 + 700 counts @14:58:12.18 SE.reconfigure SEpick @14:58:12.25 SE.seek beamBreak @14:58:12.73 delay 3 seconds @14:58:13.34 SE.configure SEconfig @14:58:13.53 Elbow.seek 4 @14:58:13.76 Forearm.seek 4 + 300 counts @14:58:13.97 Forearm.reconfigure LooseForearm @14:58:14.02 Hand.close @14:58:14.68 SE.seek :down @14:58:14.92 Forearm.seek :retract @14:58:15.52 SE.coast @14:58:15.74 ATV.dial :waste2 @14:58:15.89 Elbow.seek CC @14:58:16.48 AS.reconfigure ASconfig @14:58:16.53 AS.seek :empty @14:58:17.57 Elbow.reconfigure LooseElbow @14:58:17.69 Forearm.reconfigure TightForearm @14:58:17.74 Forearm.seek CC @14:58:19.05 CC.seek :closed @14:58:22.20 ATV.dial :air2 @14:58:22.61 AS.reconfigure ASslow1 @14:58:22.67 AS.seek 1ml @14:58:23.30 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.26 @14:58:23.32 Hand.open @14:58:23.99 Forearm.reconfigure LooseForearm @14:58:24.05 Forearm.seek :retract @14:58:25.03 SC.coast @14:58:25.07 collectSample 1500ml w/bubble point of 55psi @14:58:25.09 Priming 7ml @14:58:25.10 Intake.select :close @14:58:25.11 Exhaust.select :close @14:58:25.12 SS.hold @14:58:25.90 Calibrating pressure sensors @14:58:25.91 CTV.dial :air @14:58:27.33 SSV.dial :spit,avoiding: :exhaust @14:58:27.35 SS.reconfigure SSfast @14:58:27.41 SS.seek 5ml @14:58:30.03 SSV.dial CBV @14:58:30.06 CBV.dial SSV @14:58:31.60 delay 3.8 seconds @14:58:35.55 SSV.dial :spit @14:58:36.98 SS.seek 0ml @14:58:40.02 SSV.dial CBV @14:58:41.48 CTV.dial :intake @14:58:42.28 SSV.dial CBV @14:58:42.31 CBV.dial SSV @14:58:42.34 Allowing 40s for 7.0ml of remaining 7.0ml @14:58:42.35 Intake.select :open @14:58:44.54 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:58:44.56 SS.reconfigure SSinhale.with maxSpeed: 150 @14:58:44.61 SS.seek 7ml @14:58:49.26 Vacuum too high -- waiting up to 40s @14:58:52.67 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:58:52.70 SS.reconfigure SSinhale.with maxSpeed: 135 @14:58:52.74 SS.seek 7ml @14:58:54.34 Stroke completed -- waiting for pressure to equalize ... @14:59:00.12 Intake.select :close @14:59:00.28 SSV.dial :exhaust @14:59:01.11 Exhaust.select :open @14:59:01.22 SS.reconfigure SSexhale @14:59:01.28 SS.seek 0ml @14:59:05.02 Exhaust.select :close @14:59:05.14 Sampling 1500.0ml @14:59:05.15 Intake.select :close @14:59:05.17 Exhaust.select :close @14:59:05.18 SS.reconfigure SSconfig @14:59:05.23 SS.hold @14:59:05.45 CTV.dial :intake @14:59:05.47 SSV.dial CBV @14:59:05.50 CBV.dial SSV @14:59:06.36 Allowing 130s for 25.0ml of remaining 1500.0ml @14:59:06.37 Intake.select :open @14:59:08.55 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:59:08.57 SS.reconfigure SSinhale.with maxSpeed: 135 @14:59:08.62 SS.seek 25ml @14:59:12.70 Vacuum too high -- waiting up to 40s @14:59:14.36 SS.reconfigure SSinhale.with maxSpeed: 122 @14:59:14.42 SS.seek 25ml @14:59:17.11 Vacuum too high -- waiting up to 40s @14:59:18.75 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:59:18.77 SS.reconfigure SSinhale.with maxSpeed: 110 @14:59:18.82 SS.seek 25ml @14:59:21.64 Vacuum too high -- waiting up to 40s @14:59:23.04 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:59:23.06 SS.reconfigure SSinhale.with maxSpeed: 99 @14:59:23.11 SS.seek 25ml @14:59:25.67 Vacuum too high -- waiting up to 40s @14:59:27.05 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:59:27.07 SS.reconfigure SSinhale.with maxSpeed: 89 @14:59:27.12 SS.seek 25ml @14:59:30.11 Vacuum too high -- waiting up to 40s @14:59:31.49 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:59:31.51 SS.reconfigure SSinhale.with maxSpeed: 80 @14:59:31.56 SS.seek 25ml @14:59:34.52 Vacuum too high -- waiting up to 40s @14:59:35.65 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:59:35.67 SS.reconfigure SSinhale.with maxSpeed: 72 @14:59:35.72 SS.seek 25ml @14:59:39.01 Vacuum too high -- waiting up to 40s @14:59:40.37 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:59:40.39 SS.reconfigure SSinhale.with maxSpeed: 65 @14:59:40.44 SS.seek 25ml @14:59:44.76 Vacuum too high -- waiting up to 40s @14:59:45.87 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:59:45.89 SS.reconfigure SSinhale.with maxSpeed: 59 @14:59:45.94 SS.seek 25ml @14:59:48.46 Stroke completed -- waiting for pressure to equalize ... @14:59:51.63 Intake.select :close @14:59:51.79 SSV.dial :exhaust @14:59:52.63 Exhaust.select :open @14:59:52.74 SS.reconfigure SSexhale @14:59:52.79 SS.seek 0ml @15:00:01.97 Exhaust.select :close @15:00:02.09 SSV.dial CBV @15:00:02.12 CBV.dial SSV @15:00:02.97 Allowing 130s for 25.0ml of remaining 1475.0ml @15:00:02.98 Intake.select :open @15:00:05.16 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:00:05.18 SS.reconfigure SSinhale.with maxSpeed: 59 @15:00:05.23 SS.seek 25ml @15:00:11.64 Vacuum too high -- waiting up to 40s @15:00:12.77 SS.reconfigure SSinhale.with maxSpeed: 53 @15:00:12.83 SS.seek 25ml @15:00:22.17 Vacuum too high -- waiting up to 40s @15:00:23.29 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:00:23.31 SS.reconfigure SSinhale.with maxSpeed: 48 @15:00:23.36 SS.seek 25ml @15:00:43.54 Stroke completed -- waiting for pressure to equalize ... @15:00:46.79 Intake.select :close @15:00:46.95 SSV.dial :exhaust @15:00:47.80 Exhaust.select :open @15:00:47.91 SS.reconfigure SSexhale @15:00:47.96 SS.seek 0ml @15:00:57.10 Exhaust.select :close @15:00:57.22 SSV.dial CBV @15:00:57.25 CBV.dial SSV @15:00:58.11 Allowing 130s for 25.0ml of remaining 1450.0ml @15:00:58.12 Intake.select :open @15:01:00.31 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:01:00.33 SS.reconfigure SSinhale.with maxSpeed: 48 @15:01:00.37 SS.seek 25ml @15:01:29.74 Vacuum too high -- waiting up to 40s @15:01:30.86 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:01:30.88 SS.reconfigure SSinhale.with maxSpeed: 43 @15:01:30.93 SS.seek 25ml @15:01:39.83 Stroke completed -- waiting for pressure to equalize ... @15:01:42.44 Intake.select :close @15:01:42.61 SSV.dial :exhaust @15:01:43.44 Exhaust.select :open @15:01:43.55 SS.reconfigure SSexhale @15:01:43.60 SS.seek 0ml @15:01:44.27 delay 5 seconds @15:01:49.29 ATV.dial ASV @15:01:50.23 ASV.dial :SPEdelivery @15:01:50.25 AS.seek 0.02ml @15:01:52.85 Exhaust.select :close @15:01:52.97 SSV.dial CBV @15:01:53.02 CBV.dial SSV @15:01:53.83 Allowing 130s for 25.0ml of remaining 1425.0ml @15:01:54.38 Intake.select :open @15:01:56.57 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:01:56.59 SS.reconfigure SSinhale.with maxSpeed: 43 @15:01:56.64 SS.seek 25ml @15:02:37.77 Stroke completed -- waiting for pressure to equalize ... @15:02:40.39 Intake.select :close @15:02:40.56 SSV.dial :exhaust @15:02:41.39 Exhaust.select :open @15:02:41.51 SS.reconfigure SSexhale @15:02:41.57 SS.seek 0ml @15:02:50.55 Exhaust.select :close @15:02:50.67 SSV.dial CBV @15:02:50.70 CBV.dial SSV @15:02:51.55 Allowing 130s for 25.0ml of remaining 1400.0ml @15:02:51.56 Intake.select :open @15:02:53.75 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:02:53.77 SS.reconfigure SSinhale.with maxSpeed: 43 @15:02:53.82 SS.seek 25ml @15:03:35.57 Stroke completed -- waiting for pressure to equalize ... @15:03:38.80 Intake.select :close @15:03:38.97 SSV.dial :exhaust @15:03:39.80 Exhaust.select :open @15:03:39.91 SS.reconfigure SSexhale @15:03:39.96 SS.seek 0ml @15:03:48.82 Exhaust.select :close @15:03:48.94 SSV.dial CBV @15:03:48.97 CBV.dial SSV @15:03:49.83 Allowing 130s for 25.0ml of remaining 1375.0ml @15:03:49.84 Intake.select :open @15:03:52.02 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:03:52.04 SS.reconfigure SSinhale.with maxSpeed: 43 @15:03:52.09 SS.seek 25ml @15:04:33.79 Stroke completed -- waiting for pressure to equalize ... @15:04:37.06 Intake.select :close @15:04:37.23 SSV.dial :exhaust @15:04:38.06 Exhaust.select :open @15:04:38.17 SS.reconfigure SSexhale @15:04:38.22 SS.seek 0ml @15:04:47.27 Exhaust.select :close @15:04:47.39 SSV.dial CBV @15:04:47.42 CBV.dial SSV @15:04:48.27 Allowing 130s for 25.0ml of remaining 1350.0ml @15:04:48.28 Intake.select :open @15:04:50.46 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:04:50.48 SS.reconfigure SSinhale.with maxSpeed: 43 @15:04:50.53 SS.seek 25ml @15:05:07.63 delay 5 seconds @15:05:12.65 ATV.dial :air2 @15:05:13.68 AS.seek 0.52ml @15:05:31.79 Stroke completed -- waiting for pressure to equalize ... @15:05:35.05 Intake.select :close @15:05:35.21 SSV.dial :exhaust @15:05:36.03 Exhaust.select :open @15:05:36.14 SS.reconfigure SSexhale @15:05:36.19 SS.seek 0ml @15:05:45.27 Exhaust.select :close @15:05:45.93 SSV.dial CBV @15:05:45.95 CBV.dial SSV @15:05:46.81 Allowing 130s for 25.0ml of remaining 1325.0ml @15:05:46.82 Intake.select :open @15:05:49.01 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:05:49.03 SS.reconfigure SSinhale.with maxSpeed: 43 @15:05:49.08 SS.seek 25ml @15:06:30.60 Stroke completed -- waiting for pressure to equalize ... @15:06:33.86 Intake.select :close @15:06:34.03 SSV.dial :exhaust @15:06:34.88 Exhaust.select :open @15:06:35.00 SS.reconfigure SSexhale @15:06:35.06 SS.seek 0ml @15:06:44.01 Exhaust.select :close @15:06:44.13 SSV.dial CBV @15:06:44.16 CBV.dial SSV @15:06:45.02 Allowing 130s for 25.0ml of remaining 1300.0ml @15:06:45.03 Intake.select :open @15:06:47.22 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:06:47.24 SS.reconfigure SSinhale.with maxSpeed: 43 @15:06:47.28 SS.seek 25ml @15:06:54.53 delay 5 seconds @15:06:59.55 ATV.dial ASV @15:07:00.63 ASV.dial :wasteR @15:07:01.09 AS.seek 0.3ml @15:07:29.04 Stroke completed -- waiting for pressure to equalize ... @15:07:32.91 Intake.select :close @15:07:33.07 SSV.dial :exhaust @15:07:33.89 Exhaust.select :open @15:07:34.00 SS.reconfigure SSexhale @15:07:34.09 SS.seek 0ml @15:07:43.24 Exhaust.select :close @15:07:43.36 SSV.dial CBV @15:07:43.39 CBV.dial SSV @15:07:44.25 Allowing 130s for 25.0ml of remaining 1275.0ml @15:07:44.26 Intake.select :open @15:07:45.61 delay 20 seconds @15:07:46.44 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:07:46.46 SS.reconfigure SSinhale.with maxSpeed: 43 @15:07:46.51 SS.seek 25ml @15:08:05.64 AS.seek 0.07ml @15:08:28.01 Stroke completed -- waiting for pressure to equalize ... @15:08:31.89 Intake.select :close @15:08:32.06 SSV.dial :exhaust @15:08:32.89 Exhaust.select :open @15:08:33.00 SS.reconfigure SSexhale @15:08:33.06 SS.seek 0ml @15:08:42.15 Exhaust.select :close @15:08:42.27 SSV.dial CBV @15:08:42.30 CBV.dial SSV @15:08:43.16 Allowing 130s for 25.0ml of remaining 1250.0ml @15:08:43.17 Intake.select :open @15:08:45.36 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:08:45.38 SS.reconfigure SSinhale.with maxSpeed: 43 @15:08:45.43 SS.seek 25ml @15:08:52.17 delay 20 seconds @15:09:12.19 ATV.dial :waste2 @15:09:12.92 AS.reconfigure ASconfig @15:09:12.97 AS.seek :empty @15:09:17.37 Elution @15:09:17.38 ATV.dial :air1 @15:09:19.06 AS.reconfigure ASslow1 @15:09:19.09 Vacuum too high -- waiting up to 40s @15:09:19.15 AS.seek 0.049ml @15:09:19.97 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:09:19.99 SS.reconfigure SSinhale.with maxSpeed: 39 @15:09:20.04 SS.seek 25ml @15:09:28.83 Stroke completed -- waiting for pressure to equalize ... @15:09:29.60 delay 3 seconds @15:09:32.06 Intake.select :close @15:09:32.22 SSV.dial :exhaust @15:09:32.62 ATV.dial :ElutionWater @15:09:33.06 Exhaust.select :open @15:09:33.17 SS.reconfigure SSexhale @15:09:33.26 SS.seek 0ml @15:09:33.39 AS.seek 0.109ml @15:09:42.35 Exhaust.select :close @15:09:42.47 SSV.dial CBV @15:09:42.50 CBV.dial SSV @15:09:43.34 Allowing 130s for 25.0ml of remaining 1225.0ml @15:09:43.35 Intake.select :open @15:09:45.54 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:09:45.56 SS.reconfigure SSinhale.with maxSpeed: 39 @15:09:45.61 SS.seek 25ml @15:09:45.85 delay 3 seconds @15:09:48.87 ATV.dial ASV,via: :air2 @15:09:50.91 ASV.dial :SPEelution @15:09:51.48 AFV.select :carrierSolution @15:09:51.65 AS.reconfigure ASnorm @15:09:51.70 AS.seek 0.759ml @15:10:24.77 delay 3 seconds @15:10:27.79 AFV.select :holdingCoil @15:10:27.95 AS.reconfigure ASslow1 @15:10:28.00 AS.seek 0.474ml @15:10:31.13 Stroke completed -- waiting for pressure to equalize ... @15:10:35.03 Intake.select :close @15:10:35.19 SSV.dial :exhaust @15:10:36.02 Exhaust.select :open @15:10:36.13 SS.reconfigure SSexhale @15:10:36.20 SS.seek 0ml @15:10:45.08 Exhaust.select :close @15:10:45.20 SSV.dial CBV @15:10:45.23 CBV.dial SSV @15:10:46.08 Allowing 130s for 25.0ml of remaining 1200.0ml @15:10:46.09 Intake.select :open @15:10:48.28 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:10:48.30 SS.reconfigure SSinhale.with maxSpeed: 39 @15:10:48.35 SS.seek 25ml @15:11:25.60 delay 5 seconds @15:11:30.70 AS.reconfigure ASslow2 @15:11:30.75 AS.seek 0.439ml @15:11:33.85 Stroke completed -- waiting for pressure to equalize ... @15:11:37.75 Intake.select :close @15:11:37.92 SSV.dial :exhaust @15:11:38.77 Exhaust.select :open @15:11:38.88 SS.reconfigure SSexhale @15:11:38.94 SS.seek 0ml @15:11:48.17 Exhaust.select :close @15:11:48.29 SSV.dial CBV @15:11:48.32 CBV.dial SSV @15:11:49.19 Allowing 130s for 25.0ml of remaining 1175.0ml @15:11:49.20 Intake.select :open @15:11:51.39 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:11:51.41 SS.reconfigure SSinhale.with maxSpeed: 39 @15:11:51.46 SS.seek 25ml @15:12:06.07 delay 1:00 @15:12:37.07 Stroke completed -- waiting for pressure to equalize ... @15:12:40.94 Intake.select :close @15:12:41.10 SSV.dial :exhaust @15:12:41.92 Exhaust.select :open @15:12:42.04 SS.reconfigure SSexhale @15:12:42.11 SS.seek 0ml @15:12:50.90 Exhaust.select :close @15:12:51.02 SSV.dial CBV @15:12:51.05 CBV.dial SSV @15:12:51.91 Allowing 130s for 25.0ml of remaining 1150.0ml @15:12:51.92 Intake.select :open @15:12:54.10 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:12:54.13 SS.reconfigure SSinhale.with maxSpeed: 39 @15:12:54.17 SS.seek 25ml @15:13:06.10 pushing eluate to holding coil @15:13:06.17 AS.seek 0.279ml @15:13:40.08 Stroke completed -- waiting for pressure to equalize ... @15:13:44.60 Intake.select :close @15:13:44.76 SSV.dial :exhaust @15:13:45.62 Exhaust.select :open @15:13:45.74 SS.reconfigure SSexhale @15:13:45.79 SS.seek 0ml @15:13:54.68 Exhaust.select :close @15:13:54.80 SSV.dial CBV @15:13:54.83 CBV.dial SSV @15:13:55.70 Allowing 130s for 25.0ml of remaining 1125.0ml @15:13:55.71 Intake.select :open @15:13:57.90 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:13:57.92 SS.reconfigure SSinhale.with maxSpeed: 39 @15:13:57.97 SS.seek 25ml @15:14:43.91 Stroke completed -- waiting for pressure to equalize ... @15:14:48.41 Intake.select :close @15:14:48.58 SSV.dial :exhaust @15:14:49.42 Exhaust.select :open @15:14:49.53 SS.reconfigure SSexhale @15:14:49.58 SS.seek 0ml @15:14:59.02 Exhaust.select :close @15:14:59.14 SSV.dial CBV @15:14:59.17 CBV.dial SSV @15:15:00.03 Allowing 130s for 25.0ml of remaining 1100.0ml @15:15:00.04 Intake.select :open @15:15:02.23 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:15:02.25 SS.reconfigure SSinhale.with maxSpeed: 39 @15:15:02.30 SS.seek 25ml @15:15:38.26 Vacuum too high -- waiting up to 40s @15:15:39.65 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:15:39.67 SS.reconfigure SSinhale.with maxSpeed: 35 @15:15:39.72 SS.seek 25ml @15:15:47.21 delay 30 seconds @15:15:50.37 Stroke completed -- waiting for pressure to equalize ... @15:15:54.29 Intake.select :close @15:15:54.46 SSV.dial :exhaust @15:15:55.30 Exhaust.select :open @15:15:55.42 SS.reconfigure SSexhale @15:15:55.47 SS.seek 0ml @15:16:04.76 Exhaust.select :close @15:16:04.88 SSV.dial CBV @15:16:04.91 CBV.dial SSV @15:16:05.77 Allowing 130s for 25.0ml of remaining 1075.0ml @15:16:05.78 Intake.select :open @15:16:07.96 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:16:07.98 SS.reconfigure SSinhale.with maxSpeed: 35 @15:16:08.03 SS.seek 25ml @15:16:17.24 ATV.dial :waste,via: :air3 @15:16:18.90 AS.reconfigure ASconfig @15:16:18.95 AS.seek :empty @15:16:35.35 Parking Bleach in LysateSource line and SPEcolumn @15:16:35.36 ATV.dial :air3 @15:16:36.17 AS.reconfigure ASslow1 @15:16:36.22 AS.seek 0.029ml @15:16:42.59 ATV.dial :ElutionWater @15:16:43.22 AS.seek 0.059ml @15:16:49.53 ATV.dial :air3 @15:16:50.15 AS.seek 0.069ml @15:16:52.44 delay 2 seconds @15:16:54.45 ATV.dial :bleach @15:16:54.93 AS.seek 0.769ml @15:16:58.40 Stroke completed -- waiting for pressure to equalize ... @15:17:02.92 Intake.select :close @15:17:03.08 SSV.dial :exhaust @15:17:03.91 Exhaust.select :open @15:17:04.03 SS.reconfigure SSexhale @15:17:04.08 SS.seek 0ml @15:17:12.96 Exhaust.select :close @15:17:13.08 SSV.dial CBV @15:17:13.11 CBV.dial SSV @15:17:13.97 Allowing 130s for 25.0ml of remaining 1050.0ml @15:17:13.98 Intake.select :open @15:17:16.17 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:17:16.19 SS.reconfigure SSinhale.with maxSpeed: 35 @15:17:16.24 SS.seek 25ml @15:18:06.91 Stroke completed -- waiting for pressure to equalize ... @15:18:11.44 Intake.select :close @15:18:11.60 SSV.dial :exhaust @15:18:12.44 Exhaust.select :open @15:18:12.55 SS.reconfigure SSexhale @15:18:13.15 SS.seek 0ml @15:18:22.38 Exhaust.select :close @15:18:22.50 SSV.dial CBV @15:18:22.53 CBV.dial SSV @15:18:23.41 Allowing 130s for 25.0ml of remaining 1025.0ml @15:18:23.42 Intake.select :open @15:18:25.60 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:18:25.62 SS.reconfigure SSinhale.with maxSpeed: 35 @15:18:25.67 SS.seek 25ml @15:19:15.96 delay 3 seconds @15:19:16.16 Stroke completed -- waiting for pressure to equalize ... @15:19:18.98 ATV.dial :air3 @15:19:19.54 delay 3 seconds @15:19:21.34 Intake.select :close @15:19:21.51 SSV.dial :exhaust @15:19:22.36 Exhaust.select :open @15:19:22.47 SS.reconfigure SSexhale @15:19:22.52 SS.seek 0ml @15:19:22.64 AS.seek 0.794ml @15:19:27.99 delay 3 seconds @15:19:31.01 ATV.dial :bleach @15:19:31.62 AS.seek 0.779ml @15:19:31.69 Exhaust.select :close @15:19:31.80 SSV.dial CBV @15:19:31.83 CBV.dial SSV @15:19:32.70 Allowing 130s for 25.0ml of remaining 1000.0ml @15:19:32.71 Intake.select :open @15:19:34.92 delay 3 seconds @15:19:34.94 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:19:34.96 SS.reconfigure SSinhale.with maxSpeed: 35 @15:19:35.01 SS.seek 25ml @15:19:37.95 ATV.dial :AIV @15:19:39.18 AS.reconfigure ASconfig @15:19:39.23 AS.seek 0.015ml @15:20:23.46 ATV.dial :waste @15:20:24.91 AS.seek :empty @15:20:25.37 Stroke completed -- waiting for pressure to equalize ... @15:20:26.12 ATV.dial :air3 @15:20:26.79 AS.reconfigure ASslow1 @15:20:26.84 AS.seek 0.009ml @15:20:29.23 delay 2 seconds @15:20:30.56 Intake.select :close @15:20:30.73 SSV.dial :exhaust @15:20:31.25 ATV.dial :bleach @15:20:31.58 Exhaust.select :open @15:20:31.72 AS.seek 0.579ml @15:20:31.78 SS.reconfigure SSexhale @15:20:31.83 SS.seek 0ml @15:20:40.76 Exhaust.select :close @15:20:40.88 SSV.dial CBV @15:20:40.91 CBV.dial SSV @15:20:41.78 Allowing 130s for 25.0ml of remaining 975.0ml @15:20:41.79 Intake.select :open @15:20:43.98 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:20:44.00 SS.reconfigure SSinhale.with maxSpeed: 35 @15:20:44.05 SS.seek 25ml @15:21:27.45 Vacuum too high -- waiting up to 40s @15:21:28.85 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:21:28.87 SS.reconfigure SSinhale.with maxSpeed: 32 @15:21:28.92 SS.seek 25ml @15:21:37.15 Stroke completed -- waiting for pressure to equalize ... @15:21:41.68 Intake.select :close @15:21:41.85 SSV.dial :exhaust @15:21:42.70 Exhaust.select :open @15:21:42.83 SS.reconfigure SSexhale @15:21:42.89 SS.seek 0ml @15:21:52.10 Exhaust.select :close @15:21:52.22 SSV.dial CBV @15:21:52.25 CBV.dial SSV @15:21:53.11 Allowing 130s for 25.0ml of remaining 950.0ml @15:21:53.12 Intake.select :open @15:21:55.31 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:21:55.33 SS.reconfigure SSinhale.with maxSpeed: 32 @15:21:55.38 SS.seek 25ml @15:22:26.62 delay 3 seconds @15:22:29.64 ATV.dial :air3 @15:22:30.19 delay 3 seconds @15:22:33.28 AS.seek 0.604ml @15:22:38.58 delay 3 seconds @15:22:41.60 ATV.dial :bleach @15:22:42.22 AS.seek 0.589ml @15:22:45.52 delay 3 seconds @15:22:48.54 ATV.dial :ASV @15:22:49.55 ASV.dial :wasteR @15:22:50.12 AS.seek 0ml @15:22:50.35 Stroke completed -- waiting for pressure to equalize ... @15:22:55.51 Intake.select :close @15:22:55.67 SSV.dial :exhaust @15:22:56.50 Exhaust.select :open @15:22:56.61 SS.reconfigure SSexhale @15:22:56.66 SS.seek 0ml @15:23:05.91 Exhaust.select :close @15:23:06.03 SSV.dial CBV @15:23:06.06 CBV.dial SSV @15:23:06.91 Allowing 130s for 25.0ml of remaining 925.0ml @15:23:06.92 Intake.select :open @15:23:09.11 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:23:09.13 SS.reconfigure SSinhale.with maxSpeed: 32 @15:23:09.18 SS.seek 25ml @15:24:04.16 Stroke completed -- waiting for pressure to equalize ... @15:24:09.96 Intake.select :close @15:24:10.13 SSV.dial :exhaust @15:24:10.94 Exhaust.select :open @15:24:11.06 SS.reconfigure SSexhale @15:24:11.11 SS.seek 0ml @15:24:20.61 Exhaust.select :close @15:24:20.73 SSV.dial CBV @15:24:20.76 CBV.dial SSV @15:24:21.61 Allowing 130s for 25.0ml of remaining 900.0ml @15:24:21.62 Intake.select :open @15:24:23.80 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:24:23.82 SS.reconfigure SSinhale.with maxSpeed: 32 @15:24:23.88 SS.seek 25ml @15:24:48.88 delay 1:00 @15:25:18.21 Vacuum too high -- waiting up to 40s @15:25:19.85 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:25:19.87 SS.reconfigure SSinhale.with maxSpeed: 29 @15:25:19.92 SS.seek 25ml @15:25:21.29 Stroke completed -- waiting for pressure to equalize ... @15:25:25.83 Intake.select :close @15:25:26.00 SSV.dial :exhaust @15:25:26.83 Exhaust.select :open @15:25:26.95 SS.reconfigure SSexhale @15:25:27.01 SS.seek 0ml @15:25:36.82 Exhaust.select :close @15:25:36.94 SSV.dial CBV @15:25:36.97 CBV.dial SSV @15:25:37.83 Allowing 130s for 25.0ml of remaining 875.0ml @15:25:37.84 Intake.select :open @15:25:40.03 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:25:40.05 SS.reconfigure SSinhale.with maxSpeed: 29 @15:25:40.10 SS.seek 25ml @15:25:48.90 AFV.select :carrierSolution @15:25:49.01 delay 2 seconds @15:25:51.04 AS.reconfigure ASconfig @15:25:51.09 AS.seek 1ml @15:26:40.77 Stroke completed -- waiting for pressure to equalize ... @15:26:45.91 Intake.select :close @15:26:46.07 SSV.dial :exhaust @15:26:46.89 Exhaust.select :open @15:26:47.00 SS.reconfigure SSexhale @15:26:47.05 SS.seek 0ml @15:26:48.90 delay 1 second @15:26:49.91 AFV.select :holdingCoil @15:26:50.02 delay 1 second @15:26:51.04 ATV.dial ASV @15:26:51.06 ASV.dial :wasteR @15:26:51.08 AS.reconfigure ASslow1 @15:26:51.12 AS.seek 0ml @15:26:56.34 Exhaust.select :close @15:26:56.46 SSV.dial CBV @15:26:56.49 CBV.dial SSV @15:26:57.34 Allowing 130s for 25.0ml of remaining 850.0ml @15:26:57.35 Intake.select :open @15:26:59.54 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:26:59.56 SS.reconfigure SSinhale.with maxSpeed: 29 @15:26:59.61 SS.seek 25ml @15:27:59.93 Stroke completed -- waiting for pressure to equalize ... @15:28:05.68 Intake.select :close @15:28:05.84 SSV.dial :exhaust @15:28:06.69 Exhaust.select :open @15:28:06.80 SS.reconfigure SSexhale @15:28:06.86 SS.seek 0ml @15:28:16.13 Exhaust.select :close @15:28:16.25 SSV.dial CBV @15:28:16.28 CBV.dial SSV @15:28:17.16 Allowing 130s for 25.0ml of remaining 825.0ml @15:28:17.17 Intake.select :open @15:28:19.36 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:28:19.38 SS.reconfigure SSinhale.with maxSpeed: 29 @15:28:19.43 SS.seek 25ml @15:29:09.74 Vacuum too high -- waiting up to 40s @15:29:11.37 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:29:11.39 SS.reconfigure SSinhale.with maxSpeed: 26 @15:29:11.44 SS.seek 25ml @15:29:22.96 Stroke completed -- waiting for pressure to equalize ... @15:29:28.12 Intake.select :close @15:29:28.28 SSV.dial :exhaust @15:29:29.13 Exhaust.select :open @15:29:29.24 SS.reconfigure SSexhale @15:29:29.29 SS.seek 0ml @15:29:38.72 Exhaust.select :close @15:29:38.84 SSV.dial CBV @15:29:38.87 CBV.dial SSV @15:29:39.73 Allowing 130s for 25.0ml of remaining 800.0ml @15:29:39.74 Intake.select :open @15:29:41.93 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:29:41.95 SS.reconfigure SSinhale.with maxSpeed: 26 @15:29:42.00 SS.seek 25ml @15:30:12.51 delay 20 seconds @15:30:32.53 ATV.dial :air3 @15:30:34.00 AS.seek 0.59ml @15:30:49.54 Stroke completed -- waiting for pressure to equalize ... @15:30:55.33 Intake.select :close @15:30:55.49 SSV.dial :exhaust @15:30:56.35 Exhaust.select :open @15:30:56.48 SS.reconfigure SSexhale @15:30:56.53 SS.seek 0ml @15:31:05.57 Exhaust.select :close @15:31:05.69 SSV.dial CBV @15:31:05.72 CBV.dial SSV @15:31:06.59 Allowing 130s for 25.0ml of remaining 775.0ml @15:31:06.60 Intake.select :open @15:31:09.24 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:31:09.35 SS.reconfigure SSinhale.with maxSpeed: 26 @15:31:09.40 SS.seek 25ml @15:32:17.24 Stroke completed -- waiting for pressure to equalize ... @15:32:23.69 Intake.select :close @15:32:23.88 SSV.dial :exhaust @15:32:24.72 Exhaust.select :open @15:32:24.84 SS.reconfigure SSexhale @15:32:24.89 SS.seek 0ml @15:32:32.96 delay 3 seconds @15:32:34.10 Exhaust.select :close @15:32:34.22 SSV.dial CBV @15:32:34.25 CBV.dial SSV @15:32:35.13 Allowing 130s for 25.0ml of remaining 750.0ml @15:32:35.14 Intake.select :open @15:32:35.97 ATV.dial :ASV @15:32:37.32 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:32:37.35 SS.reconfigure SSinhale.with maxSpeed: 26 @15:32:37.40 SS.seek 25ml @15:32:37.47 ASV.dial :wasteR @15:32:37.55 AS.seek 0.09ml @15:33:44.99 Stroke completed -- waiting for pressure to equalize ... @15:33:52.03 Intake.select :close @15:33:52.19 SSV.dial :exhaust @15:33:53.03 Exhaust.select :open @15:33:53.14 SS.reconfigure SSexhale @15:33:53.19 SS.seek 0ml @15:34:02.49 Exhaust.select :close @15:34:02.61 SSV.dial CBV @15:34:02.67 CBV.dial SSV @15:34:03.47 Allowing 130s for 25.0ml of remaining 725.0ml @15:34:03.48 Intake.select :open @15:34:05.66 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:34:05.68 SS.reconfigure SSinhale.with maxSpeed: 26 @15:34:05.74 SS.seek 25ml @15:34:18.43 delay 30 seconds @15:34:48.45 ATV.dial :extSrc @15:34:49.20 delay 3 seconds @15:34:52.22 ATV.dial :ElutionWater @15:34:53.31 AS.seek 0.12ml @15:34:58.55 Vacuum too high -- waiting up to 40s @15:34:59.67 ATV.dial :air3 @15:35:00.18 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:35:00.21 SS.reconfigure SSinhale.with maxSpeed: 23 @15:35:00.27 SS.seek 25ml @15:35:00.97 AS.seek 0.13ml @15:35:03.28 delay 2 seconds @15:35:05.29 ATV.dial :bleach @15:35:05.77 AS.seek 0.23ml @15:35:16.86 Stroke completed -- waiting for pressure to equalize ... @15:35:23.30 Intake.select :close @15:35:23.47 SSV.dial :exhaust @15:35:24.31 Exhaust.select :open @15:35:24.42 SS.reconfigure SSexhale @15:35:24.48 SS.seek 0ml @15:35:26.21 delay 3 seconds @15:35:29.23 ATV.dial :air3 @15:35:29.78 delay 3 seconds @15:35:32.87 AS.seek 0.255ml @15:35:33.35 Exhaust.select :close @15:35:33.47 SSV.dial CBV @15:35:33.50 CBV.dial SSV @15:35:34.37 Allowing 130s for 25.0ml of remaining 700.0ml @15:35:34.38 Intake.select :open @15:35:36.57 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:35:36.59 SS.reconfigure SSinhale.with maxSpeed: 23 @15:35:36.64 SS.seek 25ml @15:35:38.21 delay 3 seconds @15:35:41.23 ATV.dial :bleach @15:35:41.85 AS.seek 0.24ml @15:35:45.12 delay 3 seconds @15:35:48.14 ATV.dial :ASV @15:35:49.14 ASV.dial :SPEdelivery @15:35:49.50 AFV.select :carrierSolution @15:35:49.62 delay 2 seconds @15:35:51.65 AS.reconfigure ASconfig @15:35:51.70 AS.seek 0.5ml @15:36:06.96 delay 1 second @15:36:07.97 AFV.select :holdingCoil @15:36:08.13 AS.reconfigure ASslow1 @15:36:08.18 AS.seek 0.17ml @15:36:53.27 Stroke completed -- waiting for pressure to equalize ... @15:37:00.32 Intake.select :close @15:37:00.48 SSV.dial :exhaust @15:37:01.33 Exhaust.select :open @15:37:01.44 SS.reconfigure SSexhale @15:37:01.49 SS.seek 0ml @15:37:10.83 Exhaust.select :close @15:37:10.95 SSV.dial CBV @15:37:10.98 CBV.dial SSV @15:37:11.84 Allowing 130s for 25.0ml of remaining 675.0ml @15:37:11.85 Intake.select :open @15:37:14.04 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:37:14.06 SS.reconfigure SSinhale.with maxSpeed: 23 @15:37:14.11 SS.seek 25ml @15:37:14.85 SPE.hold 2:46:40,95C @15:37:14.91 delay 3:00 @15:38:30.32 Stroke completed -- waiting for pressure to equalize ... @15:38:38.60 Intake.select :close @15:38:38.77 SSV.dial :exhaust @15:38:39.64 Exhaust.select :open @15:38:39.75 SS.reconfigure SSexhale @15:38:39.81 SS.seek 0ml @15:38:49.21 Exhaust.select :close @15:38:49.33 SSV.dial CBV @15:38:49.36 CBV.dial SSV @15:38:50.25 Allowing 130s for 25.0ml of remaining 650.0ml @15:38:50.26 Intake.select :open @15:38:52.45 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:38:52.47 SS.reconfigure SSinhale.with maxSpeed: 23 @15:38:52.52 SS.seek 25ml @15:40:05.51 Vacuum too high -- waiting up to 40s @15:40:07.41 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:40:07.44 SS.reconfigure SSinhale.with maxSpeed: 21 @15:40:07.49 SS.seek 25ml @15:40:11.18 Stroke completed -- waiting for pressure to equalize ... @15:40:15.02 AFV.select :carrierSolution @15:40:15.13 delay 2 seconds @15:40:17.15 AS.reconfigure ASconfig @15:40:17.20 AS.seek 1ml @15:40:17.63 Intake.select :close @15:40:17.80 SSV.dial :exhaust @15:40:18.67 Exhaust.select :open @15:40:18.78 SS.reconfigure SSexhale @15:40:18.83 SS.seek 0ml @15:40:28.36 Exhaust.select :close @15:40:28.48 SSV.dial CBV @15:40:28.51 CBV.dial SSV @15:40:29.39 Allowing 130s for 25.0ml of remaining 625.0ml @15:40:29.40 Intake.select :open @15:40:31.59 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:40:31.61 SS.reconfigure SSinhale.with maxSpeed: 21 @15:40:31.65 SS.seek 25ml @15:41:05.20 delay 1 second @15:41:06.22 AFV.select :holdingCoil @15:41:06.33 delay 1 second @15:41:07.35 ASV.dial :wasteD @15:41:08.47 AS.seek 0ml @15:41:55.05 Stroke completed -- waiting for pressure to equalize ... @15:42:02.71 Intake.select :close @15:42:02.87 SSV.dial :exhaust @15:42:03.73 Exhaust.select :open @15:42:03.85 SS.reconfigure SSexhale @15:42:03.90 SS.seek 0ml @15:42:06.26 Priming Eluate @15:42:06.27 AFV.select :carrierSolution @15:42:06.38 delay 2 seconds @15:42:08.42 AS.seek 0.5ml @15:42:13.41 Exhaust.select :close @15:42:13.53 SSV.dial CBV @15:42:13.56 CBV.dial SSV @15:42:14.44 Allowing 130s for 25.0ml of remaining 600.0ml @15:42:14.45 Intake.select :open @15:42:16.64 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:42:16.66 SS.reconfigure SSinhale.with maxSpeed: 21 @15:42:16.71 SS.seek 25ml @15:42:37.47 delay 1 second @15:42:38.48 AFV.select :holdingCoil @15:42:38.59 ATV.dial ASV @15:42:38.60 ASV.dial :SPErecovery @15:42:39.18 Swish #1 of 2 @15:42:39.26 AS.reconfigure ASslow0 @15:42:39.31 AS.seek 0.6ml @15:42:49.69 AS.seek 0.5ml @15:43:00.08 Swish #2 of 2 @15:43:00.17 AS.seek 0.6ml @15:43:10.66 AS.seek 0.5ml @15:43:21.03 delay 3 seconds @15:43:24.12 AS.reconfigure ASslow1 @15:43:24.17 AS.seek 0.684ml @15:43:40.40 Stroke completed -- waiting for pressure to equalize ... @15:43:49.36 Intake.select :close @15:43:49.53 SSV.dial :exhaust @15:43:50.39 Exhaust.select :open @15:43:50.50 SS.reconfigure SSexhale @15:43:50.60 SS.seek 0ml @15:43:59.91 Exhaust.select :close @15:44:00.03 SSV.dial CBV @15:44:00.06 CBV.dial SSV @15:44:00.94 Allowing 130s for 25.0ml of remaining 575.0ml @15:44:00.95 Intake.select :open @15:44:01.50 delay 3 seconds @15:44:03.14 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:44:03.16 SS.reconfigure SSinhale.with maxSpeed: 21 @15:44:03.21 SS.seek 25ml @15:44:04.52 Eluate primed @15:44:04.53 ATV.dial :waste2 @15:44:05.25 AS.reconfigure ASnorm @15:44:05.30 AS.seek 0ml @15:44:40.10 SPE.coast @15:44:40.19 ASV.dial :SPErecovery @15:44:40.21 Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1815h.pcr @15:44:40.23 Building PCR Slug from ASV with MM1 mix, PP2 primer @15:44:40.24 Priming with carrier @15:44:40.25 ATV.dial ARV @15:44:41.29 ARV.dial :carrier @15:44:41.63 AS.seek 0.5ml @15:45:07.17 AFV.select :carrierSolution @15:45:07.84 Vacuum too high -- waiting up to 40s @15:45:07.86 delay 2 seconds @15:45:09.47 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:45:09.50 SS.reconfigure SSinhale.with maxSpeed: 19 @15:45:09.55 SS.seek 25ml @15:45:09.89 AS.reconfigure ASconfig @15:45:09.94 AS.seek 1ml @15:45:31.47 Stroke completed -- waiting for pressure to equalize ... @15:45:38.97 delay 1 second @15:45:39.98 AFV.select :holdingCoil @15:45:40.10 delay 1 second @15:45:40.48 Intake.select :close @15:45:40.65 SSV.dial :exhaust @15:45:41.12 ATV.dial :waste @15:45:41.48 Exhaust.select :open @15:45:41.60 SS.reconfigure SSexhale @15:45:41.65 SS.seek 0ml @15:45:42.58 AS.reconfigure ASnorm @15:45:42.64 AS.seek :empty @15:45:51.27 Exhaust.select :close @15:45:51.39 SSV.dial CBV @15:45:51.42 CBV.dial SSV @15:45:52.28 Allowing 130s for 25.0ml of remaining 550.0ml @15:45:52.29 Intake.select :open @15:45:54.47 Intake @34.8psia, vacuum from 20.0 to 24.0psi @15:45:54.49 SS.reconfigure SSinhale.with maxSpeed: 19 @15:45:54.54 SS.seek 25ml @15:46:33.36 AFV.select :carrierSolution @15:46:33.48 delay 2 seconds @15:46:35.51 AS.reconfigure ASconfig @15:46:35.56 AS.seek 0.5ml @15:47:04.64 delay 1 second @15:47:05.65 AFV.select :holdingCoil @15:47:05.76 Building Slug @15:47:05.77 ATV.dial :ARV @15:47:07.34 delay 2 seconds @15:47:09.36 ARV.dial :air1 @15:47:09.76 delay 2 seconds @15:47:11.85 AS.reconfigure ASslow2 @15:47:11.90 AS.seek 0.506ml @15:47:18.25 delay 2 seconds @15:47:20.27 ARV.dial :MM1 @15:47:20.86 delay 2 seconds @15:47:22.89 Adding MM1 @15:47:22.96 AS.seek 0.524ml @15:47:26.76 Stroke completed -- waiting for pressure to equalize ... @15:47:35.70 Intake.select :close @15:47:35.88 SSV.dial :exhaust @15:47:36.70 Exhaust.select :open @15:47:36.82 SS.reconfigure SSexhale @15:47:36.88 SS.seek 0ml @15:47:41.44 delay 25 seconds @15:47:46.68 Exhaust.select :close @15:47:46.80 SSV.dial CBV @15:47:46.83 CBV.dial SSV @15:47:47.69 Allowing 130s for 25.0ml of remaining 525.0ml @15:47:47.70 Intake.select :open @15:47:49.89 Intake @34.9psia, vacuum from 20.0 to 24.0psi @15:47:49.91 SS.reconfigure SSinhale.with maxSpeed: 19 @15:47:49.96 SS.seek 25ml @15:48:06.46 ARV.dial :PP2 @15:48:06.86 Adding PP2 @15:48:06.88 delay 2 seconds @15:48:08.97 AS.seek 0.53ml @15:48:15.42 delay 10 seconds @15:48:25.44 ARV.dial :air2 @15:48:25.83 adding ARV air @15:48:25.85 delay 2 seconds @15:48:27.93 AS.seek 0.536ml @15:48:34.11 delay 5 seconds @15:48:39.14 ARV.dial :carrier @15:48:40.37 delay 7 seconds @15:48:47.46 AS.seek 0.594ml @15:49:02.52 Vacuum too high -- waiting up to 40s @15:49:04.41 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:49:04.43 SS.reconfigure SSinhale.with maxSpeed: 17 @15:49:04.48 SS.seek 25ml @15:49:26.77 Stroke completed -- waiting for pressure to equalize ... @15:49:35.77 Intake.select :close @15:49:35.93 SSV.dial :exhaust @15:49:36.75 Exhaust.select :open @15:49:36.87 SS.reconfigure SSexhale @15:49:36.94 SS.seek 0ml @15:49:46.01 delay 5 seconds @15:49:46.49 Exhaust.select :close @15:49:46.61 SSV.dial CBV @15:49:46.64 CBV.dial SSV @15:49:47.50 Allowing 130s for 25.0ml of remaining 500.0ml @15:49:47.51 Intake.select :open @15:49:49.69 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:49:49.71 SS.reconfigure SSinhale.with maxSpeed: 17 @15:49:49.76 SS.seek 25ml @15:49:51.03 ATV.dial :ASV @15:49:51.44 delay 2 seconds @15:49:54.10 AS.seek 0.6ml @15:50:00.42 delay 4 seconds @15:50:04.44 ATV.dial ARV @15:50:05.08 delay 2 seconds @15:50:07.10 ARV.dial :carrier @15:50:07.12 delay 2 seconds @15:50:09.22 AS.seek 0.65ml @15:50:59.83 delay 4 seconds @15:51:03.85 ATV.dial PCR @15:51:04.47 ASV.dial :waste1 @15:51:05.23 ARV.dial :air1 @15:51:05.62 Positioning Slug by 0.324ml @15:51:05.71 AS.seek 0.326ml @15:51:32.82 Stroke completed -- waiting for pressure to equalize ... @15:51:42.45 Intake.select :close @15:51:42.61 SSV.dial :exhaust @15:51:43.44 Exhaust.select :open @15:51:43.55 SS.reconfigure SSexhale @15:51:43.60 SS.seek 0ml @15:51:52.65 Exhaust.select :close @15:51:52.77 SSV.dial CBV @15:51:52.80 CBV.dial SSV @15:51:53.66 Allowing 130s for 25.0ml of remaining 475.0ml @15:51:53.67 Intake.select :open @15:51:55.86 Intake @34.8psia, vacuum from 20.0 to 24.0psi @15:51:55.88 SS.reconfigure SSinhale.with maxSpeed: 17 @15:51:55.93 SS.seek 25ml @15:53:39.68 Stroke completed -- waiting for pressure to equalize ... @15:53:50.59 Intake.select :close @15:53:50.75 SSV.dial :exhaust @15:53:51.58 Exhaust.select :open @15:53:51.69 SS.reconfigure SSexhale @15:53:51.74 SS.seek 0ml @15:54:00.93 Exhaust.select :close @15:54:01.05 SSV.dial CBV @15:54:01.08 CBV.dial SSV @15:54:02.53 Allowing 130s for 25.0ml of remaining 450.0ml @15:54:02.54 Intake.select :open @15:54:04.73 Intake @34.9psia, vacuum from 20.0 to 24.0psi @15:54:04.75 SS.reconfigure SSinhale.with maxSpeed: 17 @15:54:04.80 SS.seek 25ml @15:55:15.11 Vacuum too high -- waiting up to 40s @15:55:17.28 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:55:17.30 SS.reconfigure SSinhale.with maxSpeed: 15 @15:55:17.35 SS.seek 25ml @15:55:55.02 Stroke completed -- waiting for pressure to equalize ... @15:56:05.94 Intake.select :close @15:56:06.11 SSV.dial :exhaust @15:56:06.94 Exhaust.select :open @15:56:07.05 SS.reconfigure SSexhale @15:56:07.10 SS.seek 0ml @15:56:15.90 Exhaust.select :close @15:56:16.02 SSV.dial CBV @15:56:16.05 CBV.dial SSV @15:56:16.89 Allowing 130s for 25.0ml of remaining 425.0ml @15:56:16.90 Intake.select :open @15:56:19.09 Intake @34.8psia, vacuum from 20.0 to 24.0psi @15:56:19.11 SS.reconfigure SSinhale.with maxSpeed: 15 @15:56:19.16 SS.seek 25ml @15:56:32.06 delay 5 seconds @15:56:37.08 ATV.dial ARV @15:56:37.84 ASV.dial :PCRlock @15:56:38.22 delay 5 seconds @15:56:43.25 Running Ss Profile60C350 thermocycling @15:56:43.26 PCR.program Profile60C350 @15:56:43.41 Profile60C350 should complete in < 1:48:05 @15:56:43.42 PCR.run 2:20:06.25 @15:58:16.13 Stroke completed -- waiting for pressure to equalize ... @15:58:26.97 SS.reconfigure SSconfig @15:58:27.02 SS.hold @15:58:27.15 Intake.select :close @15:58:27.32 ShallowSampler::Clogged in WCR -- progress too slow after 1100.0ml @15:58:27.34 SSV.dial :exhaust @15:58:28.17 Exhaust.select :open @15:58:28.28 SS.reconfigure SSexhale @15:58:28.33 SS.seek 0ml @15:58:37.61 Exhaust.select :close @15:58:37.73 Sampled 1100.0ml @15:58:37.75 Drying puck before fixing for 4:00 @15:58:37.76 CBV.dial :waste2b @15:58:38.60 delay 1:00 @15:59:38.62 CSV.dial :air @15:59:39.10 CS.reconfigure CSfast @15:59:39.16 CS.seek 10ml @16:00:04.45 Pumping wcr puck from tube 4 up to 55psi... @16:00:04.46 CBV.dial SSV @16:00:05.88 CBV.dial between CSV and waste2b @16:00:06.95 CSV.dial between air and CTV @16:00:07.30 CS.seek 6.893ml @16:00:16.04 CTV.dial CSV,avoiding: :air @16:00:16.07 CSV.dial CTV @16:00:16.95 CS.reconfigure CSpressurize @16:00:17.01 CS.seek 0ml @16:00:34.56 CBV.dial :waste2b @16:00:34.93 CS.reconfigure CSconfig @16:00:34.98 CS.seek :empty @16:00:36.67 delay 1:00 @16:01:36.70 CTV.dial :air,avoiding: :intake @16:01:38.14 Fix #1 of 2 (with rnal) for 2:00 @16:01:38.16 CSV.dial CSR @16:01:38.70 Pulling 2ml of CSR.rnal @16:01:38.72 CSR.select :rnal @16:01:38.84 CS.seek 2ml @16:01:44.98 delay 5 seconds @16:01:50.00 CSR.select :air @16:01:50.13 CS.reconfigure CSfast @16:01:50.18 CS.seek 10ml @16:02:10.61 Pumping wcr puck from tube 4 up to 55psi... @16:02:10.63 CBV.dial SSV @16:02:12.00 CBV.dial between CSV and waste2b @16:02:13.05 CSV.dial between air and CTV @16:02:14.30 CS.seek 6.834ml @16:02:22.95 CTV.dial CSV,avoiding: :air @16:02:22.98 CSV.dial CTV @16:02:24.48 CS.reconfigure CSpressurize @16:02:24.54 CS.seek 0ml @16:02:41.41 delay 2:00 @16:04:41.43 CBV.dial :waste2b @16:04:41.80 CS.reconfigure CSconfig @16:04:41.85 CS.seek :empty @16:04:43.95 delay 1:00 @16:05:43.98 CTV.dial :air,avoiding: :intake @16:05:45.42 Fix #2 of 2 (with rnal) for 2:00 @16:05:45.44 CSV.dial CSR @16:05:45.99 Pulling 2ml of CSR.rnal @16:05:46.00 CSR.select :rnal @16:05:46.12 CS.seek 2ml @16:05:52.23 delay 5 seconds @16:05:57.25 CSR.select :air @16:05:57.38 CS.reconfigure CSfast @16:05:57.43 CS.seek 10ml @16:06:17.83 Pumping wcr puck from tube 4 up to 55psi... @16:06:17.84 CBV.dial SSV @16:06:19.24 CBV.dial between CSV and waste2b @16:06:20.30 CSV.dial between air and CTV @16:06:20.96 CS.seek 6.826ml @16:06:29.61 CTV.dial CSV,avoiding: :air @16:06:29.64 CSV.dial CTV @16:06:31.12 CS.reconfigure CSpressurize @16:06:31.18 CS.seek 0ml @16:06:47.91 delay 2:00 @16:08:47.94 CBV.dial :waste2b @16:08:48.30 CS.reconfigure CSconfig @16:08:48.35 CS.seek :empty @16:08:50.52 delay 1:00 @16:09:50.55 CTV.dial :air,avoiding: :intake @16:09:52.61 Storing wcr puck in Collection Clamp to tube 3 @16:09:52.84 SC.reconfigure SCtight @16:09:52.89 SC.seek 3 @16:09:55.03 SC.reconfigure SCloose @16:09:55.22 Elbow.reconfigure TightElbow @16:09:55.25 SE.reconfigure SEplace @16:09:55.37 Elbow.seek CC @16:09:55.48 SE.seek beamBreak @16:09:55.55 Elbow.reconfigure LooseElbow @16:09:55.68 Forearm.seek CC @16:09:56.67 Hand.close @16:09:57.38 CC.seek :open @16:09:58.36 SE.configure SEconfig @16:09:58.42 SE.seek :place @16:10:01.91 Forearm.seek :retract @16:10:02.90 Elbow.reconfigure TightElbow @16:10:02.95 Elbow.seek 3 @16:10:04.87 Elbow.reconfigure LimpElbow @16:10:04.94 Forearm.seek 3 @16:10:05.96 Elbow.hold @16:10:06.04 Elbow.reconfigure TightElbow @16:10:06.09 Hand.open @16:10:07.03 Forearm.force -20 @16:10:07.08 Forearm.seek 3 + 349 counts @16:10:07.42 Forearm.reconfigure TightForearm @16:10:07.48 Forearm.seek 3 + 700 counts @16:10:07.86 SE.seek :down @16:10:09.01 Forearm.reconfigure LooseForearm @16:10:09.07 Forearm.seek :retract @16:10:10.62 SE.coast @16:10:10.66 SC.coast @16:10:10.71 Starting finalPurge @16:10:10.72 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:10:10.80 Hand.open @16:10:11.17 Elbow.seek FlushPuck::Garage @16:10:13.19 Elbow.reconfigure LooseElbow @16:10:13.29 Forearm.seek :garage @16:10:14.31 Hand.close @16:10:15.01 Forearm.seek :retract @16:10:16.03 Elbow.reconfigure TightElbow @16:10:16.08 Elbow.seek CC @16:10:19.05 Elbow.reconfigure LooseElbow @16:10:19.16 Forearm.reconfigure TightForearm @16:10:19.21 Forearm.seek CC @16:10:21.09 CC.seek :closed @16:10:25.35 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.19 @16:10:25.44 Hand.open @16:10:26.08 Forearm.reconfigure LooseForearm @16:10:26.13 Forearm.seek :retract @16:10:27.10 Flushing the Collection Valve::Manifold @16:10:27.24 CSV.dial CSR @16:10:27.79 Pulling 5ml of CSR.flush @16:10:27.80 CSR.select :flush @16:10:27.92 CS.seek 5ml @16:10:41.68 delay 5 seconds @16:10:46.70 CSR.select :air @16:10:46.88 CS.reconfigure CSfast @16:10:46.93 CS.seek 7.5ml @16:10:54.07 CSV.dial :waste @16:10:55.47 CS.reconfigure CSconfig @16:10:55.52 CS.seek 0ml @16:11:15.74 Flushing lower manifold's waste1 port @16:11:15.88 CSV.dial :flush @16:11:16.36 CS.reconfigure CSfast @16:11:16.41 CS.seek 5ml @16:11:29.64 delay 5 seconds @16:11:34.65 CSV.dial :air @16:11:35.21 CS.seek 7ml @16:11:41.02 CSV.dial CTV @16:11:41.05
CTV.dial CSV @16:11:42.55 CBV.dial :waste1 @16:11:43.40 CS.seek 0ml @16:12:01.11 Flushing lower manifold's waste2b port @16:12:01.25 CSV.dial :flush @16:12:02.06 CS.seek 5ml @16:12:15.11 delay 5 seconds @16:12:20.12 CSV.dial :air @16:12:20.70 CS.seek 7ml @16:12:26.61 CSV.dial CTV @16:12:26.66
CTV.dial CSV @16:12:27.11 CBV.dial :waste2b @16:12:27.95 CS.seek 0ml @16:12:45.65 Flushing upper manifold @16:12:45.79 CSV.dial :flush @16:12:46.61 CS.seek 5ml @16:12:59.61 delay 5 seconds @16:13:04.63 CSV.dial :air @16:13:05.22 CS.seek 7ml @16:13:11.02 CSV.dial CBV @16:13:11.05
CBV.dial CSV @16:13:12.22 CTV.dial :air @16:13:14.25 CS.seek 0ml @16:13:32.02 delay 7 seconds @16:13:39.04 CS.seek 3ml @16:13:47.21 CSV.dial :waste @16:13:47.72 CS.seek 0ml @16:13:56.00 CSV.dial :flush @16:13:56.48 CS.seek 3ml @16:14:04.62 delay 5 seconds @16:14:09.63 CSV.dial :air @16:14:10.20 CS.seek 5ml @16:14:16.27 CSV.dial :air @16:14:16.29 CS.seek 0ml @16:14:29.27 delay 7 seconds @16:14:36.29 CS.seek 3ml @16:14:44.54 CSV.dial :waste @16:14:45.36 CS.seek 0ml @16:14:53.55 Disinfecting external Intake with mfbkill @16:14:53.69 CSV.dial CSR @16:14:55.15 Pulling 8ml of CSR.mfbkill @16:14:55.16 CSR.select :mfbkill @16:14:55.29 CS.seek 8ml @16:15:15.62 delay 5 seconds @16:15:20.64 CSR.select :air @16:15:20.77 CS.seek 10ml @16:15:26.55 CSV.dial CBV @16:15:26.58
CBV.dial CSV @16:15:27.69 CTV.dial :intake @16:15:28.49 Intake.select :open @16:15:28.61 CS.seek 2ml @16:15:49.48 Intake.select :close @16:15:49.60 CTV.dial :air @16:15:50.41 CS.reconfigure CSconfig @16:15:50.46 CS.seek 0ml @16:15:56.62 Flushing lower manifold's waste1 port @16:15:56.63 CSV.dial CSR @16:15:57.80 Pulling 4ml of CSR.mfbkill @16:15:57.81 CSR.select :mfbkill @16:15:57.94 CS.seek 4ml @16:16:09.12 delay 5 seconds @16:16:14.14 CSR.select :air @16:16:14.27 CS.reconfigure CSfast @16:16:14.32 CS.seek 10ml @16:16:29.68 CSV.dial CTV @16:16:29.71
CTV.dial CSV @16:16:31.21 CBV.dial :waste1 @16:16:31.71 CS.seek 0ml @16:16:56.68 Flushing lower manifold's waste2b port @16:16:56.69 CSV.dial CSR @16:16:57.26 Pulling 4ml of CSR.mfbkill @16:16:57.27 CSR.select :mfbkill @16:16:57.40 CS.reconfigure CSconfig @16:16:57.44 CS.seek 4ml @16:17:08.74 delay 5 seconds @16:17:13.75 CSR.select :air @16:17:13.88 CS.reconfigure CSfast @16:17:13.93 CS.seek 10ml @16:17:29.27 CSV.dial CTV @16:17:29.30
CTV.dial CSV @16:17:29.81 CBV.dial :waste2b @16:17:30.66 CS.seek 0ml @16:17:56.34 Killing upper manifold @16:17:56.35 CSV.dial CSR @16:17:56.92 Pulling 4ml of CSR.mfbkill @16:17:56.93 CSR.select :mfbkill @16:17:57.05 CS.reconfigure CSconfig @16:17:57.10 CS.seek 4ml @16:18:08.37 delay 5 seconds @16:18:13.39 CSR.select :air @16:18:13.52 CS.reconfigure CSfast @16:18:13.57 CS.seek 10ml @16:18:28.90 CSV.dial CBV @16:18:28.93
CBV.dial CSV @16:18:30.09 CTV.dial :air @16:18:31.54 CS.seek 0ml @16:18:56.43 delay 7 seconds @16:19:03.45 CS.seek 3ml @16:19:11.58 CSV.dial :waste @16:19:12.08 CS.seek 0ml @16:19:20.28 Flushing upper manifold @16:19:20.42 CSV.dial :flush @16:19:20.91 CS.seek 5ml @16:19:33.93 delay 5 seconds @16:19:38.95 CSV.dial :air @16:19:39.50 CS.seek 7ml @16:19:45.28 CSV.dial CBV @16:19:45.31
CBV.dial CSV @16:19:46.42 CTV.dial :air @16:19:46.44 CS.seek 0ml @16:20:04.03 delay 7 seconds @16:20:11.05 CS.seek 3ml @16:20:19.27 CSV.dial :waste @16:20:19.78 CS.seek 0ml @16:20:28.35 CSV.dial :flush @16:20:28.83 CS.seek 3ml @16:20:37.09 delay 5 seconds @16:20:42.10 CSV.dial :air @16:20:42.68 CS.seek 5ml @16:20:48.46 CSV.dial :air @16:20:48.49 CS.seek 0ml @16:21:01.40 delay 7 seconds @16:21:08.42 CS.seek 3ml @16:21:16.55 CSV.dial :waste @16:21:17.38 CS.seek 0ml @16:21:25.51 Flushing lower manifold's waste1 port @16:21:25.65 CSV.dial :flush @16:21:26.15 CS.seek 5ml @16:21:39.18 delay 5 seconds @16:21:44.19 CSV.dial :air @16:21:44.76 CS.seek 7ml @16:21:50.50 CSV.dial CTV @16:21:50.53
CTV.dial CSV @16:21:52.04 CBV.dial :waste1 @16:21:52.54 CS.seek 0ml @16:22:10.22 Flushing lower manifold's waste2b port @16:22:10.36 CSV.dial :flush @16:22:11.76 CS.seek 5ml @16:22:24.81 delay 5 seconds @16:22:29.83 CSV.dial :air @16:22:30.41 CS.seek 7ml @16:22:36.19 CSV.dial CTV @16:22:36.22
CTV.dial CSV @16:22:36.72 CBV.dial :waste2b @16:22:37.57 CS.seek 0ml @16:22:55.25 Disinfecting external Intake with kill @16:22:55.39 CSV.dial CSR @16:22:55.94 Pulling 8ml of CSR.kill @16:22:55.95 CSR.select :kill @16:22:56.07 CS.seek 8ml @16:23:16.09 delay 5 seconds @16:23:21.11 CSR.select :air @16:23:21.25 CS.seek 10ml @16:23:27.00 CSV.dial CBV @16:23:27.03
CBV.dial CSV @16:23:28.19 CTV.dial :intake @16:23:29.00 Intake.select :open @16:23:29.12 CS.seek 2ml @16:23:50.24 Intake.select :close @16:23:50.36 CTV.dial :air @16:23:51.17 CS.reconfigure CSconfig @16:23:51.22 CS.seek 0ml @16:23:57.40 CSV.dial CSR @16:23:58.57 Pulling 5ml of CSR.flush @16:23:58.58 CSR.select :flush @16:23:58.71 CS.seek 5ml @16:24:12.41 delay 5 seconds @16:24:17.43 CSR.select :air @16:24:17.56 CS.seek 10ml @16:24:31.30 CSV.dial CBV @16:24:32.41 CS.seek 0ml @16:24:58.88 Garaging FlushPuck in Collection Clamp @16:24:58.96 Elbow.reconfigure TightElbow @16:24:59.01 Elbow.seek CC @16:24:59.27 Elbow.reconfigure LooseElbow @16:24:59.37 Forearm.seek CC @16:25:00.36 Hand.close @16:25:01.16 CC.seek :open @16:25:05.54 Forearm.seek :retract @16:25:06.56 Elbow.reconfigure TightElbow @16:25:06.61 Elbow.seek :garage @16:25:09.75 Elbow.reconfigure LooseElbow @16:25:09.86 Forearm.reconfigure TightForearm @16:25:09.91 Forearm.seek :garage @16:25:11.08 Hand.open @16:25:11.85 Forearm.reconfigure LooseForearm @16:25:11.90 Forearm.seek :retract @16:25:12.92 Starting initialPurge @16:25:12.93 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:25:13.01 Hand.open @16:25:13.35 Elbow.reconfigure TightElbow @16:25:13.40 Elbow.seek FlushPuck::Garage @16:25:13.61 Elbow.reconfigure LooseElbow @16:25:13.72 Forearm.seek :garage @16:25:15.31 Hand.close @16:25:16.05 Forearm.seek :retract @16:25:17.05 Elbow.reconfigure TightElbow @16:25:17.10 Elbow.seek CC @16:25:20.04 Elbow.reconfigure LooseElbow @16:25:20.15 Forearm.reconfigure TightForearm @16:25:20.20 Forearm.seek CC @16:25:21.51 CC.seek :closed @16:25:25.77 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.18 @16:25:25.86 Hand.open @16:25:26.96 Forearm.reconfigure LooseForearm @16:25:27.11 Forearm.seek :retract @16:25:28.13 Flushing the Collection Valve::Manifold @16:25:28.25 CSV.dial CSR @16:25:29.41 Pulling 5ml of CSR.flush @16:25:29.42 CSR.select :flush @16:25:29.54 CS.seek 5ml @16:25:43.66 delay 5 seconds @16:25:48.68 CSR.select :air @16:25:48.95 CS.reconfigure CSfast @16:25:49.09 CS.seek 7.5ml @16:25:56.18 CSV.dial :waste @16:25:57.60 CS.reconfigure CSconfig @16:25:57.65 CS.seek 0ml @16:26:17.78 Purging external Intake @16:26:17.91 CSV.dial :flush @16:26:18.41 CS.reconfigure CSfast @16:26:18.46 CS.seek 10ml @16:26:43.38 delay 5 seconds @16:26:48.40 CSV.dial CBV @16:26:48.44
CBV.dial CSV @16:26:49.25 CTV.dial :intake @16:26:50.06 Intake.select :open @16:26:50.18 CS.seek 0ml @16:27:16.24 Intake.select :close @16:27:16.36 CTV.dial :air @16:27:17.17 Flushing upper manifold @16:27:17.30 CSV.dial :flush @16:27:18.12 CS.seek 5ml @16:27:31.03 delay 5 seconds @16:27:36.04 CSV.dial :air @16:27:36.60 CS.seek 7ml @16:27:42.38 CSV.dial CBV @16:27:42.40
CBV.dial CSV @16:27:43.53 CTV.dial :air @16:27:43.55 CS.seek 0ml @16:28:01.28 delay 7 seconds @16:28:08.30 CS.seek 3ml @16:28:16.49 CSV.dial :waste @16:28:17.00 CS.seek 0ml @16:28:25.34 CSV.dial :flush @16:28:25.83 CS.seek 3ml @16:28:34.03 delay 5 seconds @16:28:39.04 CSV.dial :air @16:28:39.59 CS.seek 5ml @16:28:45.27 CSV.dial :air @16:28:45.30 CS.seek 0ml @16:28:58.07 delay 7 seconds @16:29:05.10 CS.seek 3ml @16:29:13.37 CSV.dial :waste @16:29:14.19 CS.seek 0ml @16:29:22.38 Garaging FlushPuck in Collection Clamp @16:29:22.46 Elbow.reconfigure TightElbow @16:29:22.51 Elbow.seek CC @16:29:22.77 Elbow.reconfigure LooseElbow @16:29:22.89 Forearm.seek CC @16:29:23.86 Hand.close @16:29:24.66 CC.seek :open @16:29:29.01 Forearm.seek :retract @16:29:30.01 Elbow.reconfigure TightElbow @16:29:30.06 Elbow.seek :garage @16:29:33.16 Elbow.reconfigure LooseElbow @16:29:33.27 Forearm.reconfigure TightForearm @16:29:33.32 Forearm.seek :garage @16:29:34.47 Hand.open @16:29:35.23 Forearm.reconfigure LooseForearm @16:29:35.28 Forearm.seek :retract @16:29:36.31 Completed [coreNEG] begun 14:00:27 @16:29:36.33 Preparing to sleep until 14:00 19-oct-18 @16:29:36.38 deferred until MFBPCR finish @16:29:36.40
Waiting for MFBPCR til 14:00 19-oct-18 @16:29:36.43 Continuing at 14:00CEST19-Oct-18 @16:29:36.49 GoodNews.email "Then, in tube 4: mbaPhase\n/home/esp/esp2/mission/18tundeployv4.rb:122\n{1 => 22, 2 => 22, 3 => 1, 4 => 21}\nCan@16:29:36, 14.2C, 71% humidity, 14.3psia, 12.802V, 0.638A, 0.632A avg, 8.17W",Subject: "Waiting until 14:00CEST19-Oct-18" @17:19:37.62 PCR.off @17:19:39.75 PCR for Ss completed -- closed /var/log/esp/Ss18oct1815h.pcr @17:19:39.82 Cleaning PCR @17:19:39.83 AFV.select :carrierSolution @17:19:39.94 delay 2 seconds @17:19:41.97 AS.reconfigure ASconfig @17:19:42.02 AS.seek 0.5ml @17:19:52.31 delay 1 second @17:19:53.32 AFV.select :holdingCoil @17:19:53.43 ASV.dial :waste1 @17:19:53.90 ATV.dial :air3 @17:19:55.13 AS.reconfigure ASslow1 @17:19:55.18 AS.seek 0.51ml @17:19:57.45 delay 2 seconds @17:19:59.47 ATV.dial :bleach @17:20:00.06 AS.seek 0.81ml @17:21:00.67 delay 3 seconds @17:21:03.69 ATV.dial :air3 @17:21:04.21 delay 3 seconds @17:21:07.30 AS.seek 0.835ml @17:21:12.71 delay 3 seconds @17:21:15.73 ATV.dial :bleach @17:21:16.35 AS.seek 0.82ml @17:21:19.65 delay 3 seconds @17:21:22.67 ARV.dial :waste @17:21:23.26 delay 3 seconds @17:21:26.28 ATV.dial :ARV @17:21:27.23 AS.reconfigure ASconfig @17:21:27.29 AS.seek 0.47ml @17:21:47.67 AFV.select :carrierSolution @17:21:47.79 delay 2 seconds @17:21:49.82 AS.seek 1ml @17:22:20.59 delay 1 second @17:22:21.60 AFV.select :holdingCoil @17:22:21.71 delay 1 second @17:22:22.74 AS.reconfigure ASfast @17:22:22.79 AS.seek 0ml @17:22:50.13 AFV.select :carrierSolution @17:22:50.25 delay 2 seconds @17:22:52.28 AS.reconfigure ASconfig @17:22:52.32 AS.seek 1ml @17:23:50.15 delay 1 second @17:23:51.16 AFV.select :holdingCoil @17:23:51.27 delay 1 second @17:23:52.30 AS.reconfigure ASfast @17:23:52.35 AS.seek 0ml @17:24:19.69 AFV.select :carrierSolution @17:24:19.81 delay 2 seconds @17:24:21.84 AS.reconfigure ASconfig @17:24:21.89 AS.seek 1ml @17:25:19.71 delay 1 second @17:25:20.72 AFV.select :holdingCoil @17:25:20.83 delay 1 second @17:25:21.86 AS.reconfigure ASfast @17:25:21.91 AS.seek 0ml @17:25:49.25 ARV.dial between carrier and air1 @17:25:49.75 ATV.dial :air3 @17:25:50.96 AS.reconfigure ASslow1 @17:25:51.02 AS.seek 0.01ml @17:25:53.37 delay 2 seconds @17:25:55.38 ATV.dial :bleach @17:25:56.00 AS.seek 0.81ml @17:28:37.14 delay 3 seconds @17:28:40.16 ATV.dial :air3 @17:28:40.70 delay 3 seconds @17:28:43.78 AS.seek 0.835ml @17:28:49.08 delay 3 seconds @17:28:52.10 ATV.dial :bleach @17:28:52.72 AS.seek 0.82ml @17:28:55.97 delay 3 seconds @17:28:58.99 ATV.dial PCR @17:28:59.37 AS.reconfigure ASconfig @17:28:59.42 AS.seek :empty @17:29:46.86 PCR.program :bleachProfile @17:29:46.93 PCR program should complete in < 2:45 @17:29:46.94 PCR.run 10:00 @17:32:58.39 PCR.off @17:32:58.42 AFV.select :carrierSolution @17:32:58.53 delay 2 seconds @17:33:00.56 AS.seek 1ml @17:33:58.40 delay 1 second @17:33:59.41 AFV.select :holdingCoil @17:33:59.52 delay 1 second @17:34:00.55 AS.reconfigure ASfast @17:34:00.60 AS.seek 0ml @17:34:27.94 delay 3 seconds @17:34:30.96 AFV.select :carrierSolution @17:34:31.07 delay 2 seconds @17:34:33.10 AS.reconfigure ASconfig @17:34:33.15 AS.seek 1ml @17:35:30.94 delay 1 second @17:35:31.95 AFV.select :holdingCoil @17:35:32.06 delay 1 second @17:35:33.09 AS.reconfigure ASfast @17:35:33.14 AS.seek 0ml @17:36:00.50 delay 3 seconds @17:36:03.52 AFV.select :carrierSolution @17:36:03.63 delay 2 seconds @17:36:05.66 AS.reconfigure ASconfig @17:36:05.72 AS.seek 1ml @17:37:03.56 delay 1 second @17:37:04.57 AFV.select :holdingCoil @17:37:04.68 delay 1 second @17:37:05.71 AS.reconfigure ASfast @17:37:05.76 AS.seek 0ml @17:37:33.09 delay 3 seconds @17:37:36.11 AFV.select :carrierSolution @17:37:36.22 delay 2 seconds @17:37:38.25 AS.reconfigure ASconfig @17:37:38.30 AS.seek 1ml @17:38:36.09 delay 1 second @17:38:37.10 AFV.select :holdingCoil @17:38:37.21 delay 1 second @17:38:38.24 AS.reconfigure ASfast @17:38:38.29 AS.seek 0ml @17:39:05.63 delay 3 seconds @17:39:08.65 AFV.select :carrierSolution @17:39:08.76 delay 2 seconds @17:39:10.79 AS.reconfigure ASconfig @17:39:10.85 AS.seek 1ml @17:40:08.63 delay 1 second @17:40:09.64 AFV.select :holdingCoil @17:40:09.75 delay 1 second @17:40:10.78 AS.reconfigure ASnorm @17:40:10.83 AS.seek 0ml @17:41:01.57 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1817h.pcr @17:41:01.59 Building PCR Slug from ASV with MM2 mix, PP4 primer @17:41:01.61 Priming with carrier @17:41:01.62 ATV.dial ARV @17:41:02.30 ARV.dial :carrier @17:41:02.65 AS.seek 0.5ml @17:41:28.16 AFV.select :carrierSolution @17:41:28.28 delay 2 seconds @17:41:30.31 AS.reconfigure ASconfig @17:41:30.36 AS.seek 1ml @17:41:59.41 delay 1 second @17:42:00.42 AFV.select :holdingCoil @17:42:00.53 delay 1 second @17:42:01.55 ATV.dial :waste @17:42:03.11 AS.reconfigure ASnorm @17:42:03.17 AS.seek :empty @17:42:53.90 AFV.select :carrierSolution @17:42:54.02 delay 2 seconds @17:42:56.05 AS.reconfigure ASconfig @17:42:56.10 AS.seek 0.5ml @17:43:25.17 delay 1 second @17:43:26.18 AFV.select :holdingCoil @17:43:26.29 Building Slug @17:43:26.30 ATV.dial :ARV @17:43:27.83 delay 2 seconds @17:43:29.85 ARV.dial :air1 @17:43:30.25 delay 2 seconds @17:43:32.33 AS.reconfigure ASslow2 @17:43:32.38 AS.seek 0.506ml @17:43:38.66 delay 2 seconds @17:43:40.67 ARV.dial :MM2 @17:43:42.08 delay 2 seconds @17:43:44.11 Adding MM2 @17:43:44.18 AS.seek 0.524ml @17:44:02.56 delay 25 seconds @17:44:27.58 ARV.dial :PP4 @17:44:27.97 Adding PP4 @17:44:27.98 delay 2 seconds @17:44:30.06 AS.seek 0.53ml @17:44:36.33 delay 10 seconds @17:44:46.36 ARV.dial :air3 @17:44:46.74 adding ARV air @17:44:46.75 delay 2 seconds @17:44:48.84 AS.seek 0.536ml @17:44:55.02 delay 5 seconds @17:45:00.05 ARV.dial :carrier @17:45:01.27 delay 7 seconds @17:45:08.36 AS.seek 0.594ml @17:46:06.89 delay 5 seconds @17:46:11.91 ATV.dial :ASV @17:46:12.89 delay 2 seconds @17:46:14.98 AS.seek 0.6ml @17:46:21.28 delay 4 seconds @17:46:25.30 ATV.dial ARV @17:46:25.83 delay 2 seconds @17:46:27.85 ARV.dial :carrier @17:46:27.87 delay 2 seconds @17:46:29.97 AS.seek 0.65ml @17:47:20.72 delay 4 seconds @17:47:24.74 ATV.dial PCR @17:47:25.33 ASV.dial :waste1 @17:47:25.34 ARV.dial :air1 @17:47:25.73 Positioning Slug by 0.324ml @17:47:25.82 AS.seek 0.326ml @17:52:51.69 delay 5 seconds @17:52:56.71 ATV.dial ARV @17:52:57.46 ASV.dial :PCRlock @17:52:57.85 delay 5 seconds @17:53:02.88 Running Tt1 Profile60C300 thermocycling @17:53:02.89 PCR.program Profile60C300 @17:53:03.03 Profile60C300 should complete in < 1:45:21 @17:53:03.04 PCR.run 2:16:41.25 @19:15:05.44 PCR.off @19:15:07.02 PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1817h.pcr @19:15:07.09 Cleaning PCR @19:15:07.10 AFV.select :carrierSolution @19:15:07.21 delay 2 seconds @19:15:09.24 AS.reconfigure ASconfig @19:15:09.29 AS.seek 0.5ml @19:15:19.61 delay 1 second @19:15:20.62 AFV.select :holdingCoil @19:15:20.73 ASV.dial :waste1 @19:15:21.11 ATV.dial :air3 @19:15:22.33 AS.reconfigure ASslow1 @19:15:22.39 AS.seek 0.51ml @19:15:24.64 delay 2 seconds @19:15:26.66 ATV.dial :bleach @19:15:27.26 AS.seek 0.81ml @19:16:27.85 delay 3 seconds @19:16:30.87 ATV.dial :air3 @19:16:31.39 delay 3 seconds @19:16:34.47 AS.seek 0.835ml @19:16:39.77 delay 3 seconds @19:16:42.79 ATV.dial :bleach @19:16:43.40 AS.seek 0.82ml @19:16:46.68 delay 3 seconds @19:16:49.70 ARV.dial :waste @19:16:50.30 delay 3 seconds @19:16:53.32 ATV.dial :ARV @19:16:54.27 AS.reconfigure ASconfig @19:16:54.32 AS.seek 0.47ml @19:17:14.70 AFV.select :carrierSolution @19:17:14.82 delay 2 seconds @19:17:16.85 AS.seek 1ml @19:17:47.59 delay 1 second @19:17:48.61 AFV.select :holdingCoil @19:17:48.72 delay 1 second @19:17:49.75 AS.reconfigure ASfast @19:17:49.80 AS.seek 0ml @19:18:17.13 AFV.select :carrierSolution @19:18:17.25 delay 2 seconds @19:18:19.28 AS.reconfigure ASconfig @19:18:19.32 AS.seek 1ml @19:19:17.07 delay 1 second @19:19:18.09 AFV.select :holdingCoil @19:19:18.20 delay 1 second @19:19:19.23 AS.reconfigure ASfast @19:19:19.28 AS.seek 0ml @19:19:46.59 AFV.select :carrierSolution @19:19:46.71 delay 2 seconds @19:19:48.74 AS.reconfigure ASconfig @19:19:48.78 AS.seek 1ml @19:20:46.57 delay 1 second @19:20:47.59 AFV.select :holdingCoil @19:20:47.70 delay 1 second @19:20:48.73 AS.reconfigure ASfast @19:20:48.78 AS.seek 0ml @19:21:16.10 ARV.dial between carrier and air1 @19:21:16.58 ATV.dial :air3 @19:21:17.78 AS.reconfigure ASslow1 @19:21:17.83 AS.seek 0.01ml @19:21:20.19 delay 2 seconds @19:21:22.20 ATV.dial :bleach @19:21:22.81 AS.seek 0.81ml @19:24:03.96 delay 3 seconds @19:24:06.98 ATV.dial :air3 @19:24:07.51 delay 3 seconds @19:24:10.59 AS.seek 0.835ml @19:24:15.91 delay 3 seconds @19:24:18.93 ATV.dial :bleach @19:24:19.53 AS.seek 0.82ml @19:24:22.82 delay 3 seconds @19:24:25.85 ATV.dial PCR @19:24:26.29 AS.reconfigure ASconfig @19:24:26.35 AS.seek :empty @19:25:13.81 PCR.program :bleachProfile @19:25:13.88 PCR program should complete in < 2:45 @19:25:13.89 PCR.run 10:00 @19:28:25.36 PCR.off @19:28:25.38 AFV.select :carrierSolution @19:28:25.50 delay 2 seconds @19:28:27.53 AS.seek 1ml @19:29:25.37 delay 1 second @19:29:26.38 AFV.select :holdingCoil @19:29:26.49 delay 1 second @19:29:27.52 AS.reconfigure ASfast @19:29:27.57 AS.seek 0ml @19:29:54.91 delay 3 seconds @19:29:57.93 AFV.select :carrierSolution @19:29:58.04 delay 2 seconds @19:30:00.07 AS.reconfigure ASconfig @19:30:00.12 AS.seek 1ml @19:30:57.90 delay 1 second @19:30:58.92 AFV.select :holdingCoil @19:30:59.03 delay 1 second @19:31:00.06 AS.reconfigure ASfast @19:31:00.11 AS.seek 0ml @19:31:27.45 delay 3 seconds @19:31:30.47 AFV.select :carrierSolution @19:31:30.58 delay 2 seconds @19:31:32.61 AS.reconfigure ASconfig @19:31:32.66 AS.seek 1ml @19:32:30.48 delay 1 second @19:32:31.49 AFV.select :holdingCoil @19:32:31.60 delay 1 second @19:32:32.63 AS.reconfigure ASfast @19:32:32.68 AS.seek 0ml @19:33:00.02 delay 3 seconds @19:33:03.03 AFV.select :carrierSolution @19:33:03.14 delay 2 seconds @19:33:05.17 AS.reconfigure ASconfig @19:33:05.23 AS.seek 1ml @19:34:03.02 delay 1 second @19:34:04.03 AFV.select :holdingCoil @19:34:04.14 delay 1 second @19:34:05.17 AS.reconfigure ASfast @19:34:05.22 AS.seek 0ml @19:34:32.56 delay 3 seconds @19:34:35.58 AFV.select :carrierSolution @19:34:35.69 delay 2 seconds @19:34:37.72 AS.reconfigure ASconfig @19:34:37.77 AS.seek 1ml @19:35:35.56 delay 1 second @19:35:36.58 AFV.select :holdingCoil @19:35:36.69 delay 1 second @19:35:37.72 AS.reconfigure ASnorm @19:35:37.77 AS.seek 0ml @19:36:28.48 Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1819h.pcr @19:36:28.50 Building PCR Slug from ASV with MM1 mix, PP1 primer @19:36:28.51 Priming with carrier @19:36:28.52 ATV.dial ARV @19:36:29.20 ARV.dial :carrier @19:36:29.55 AS.seek 0.5ml @19:36:55.16 AFV.select :carrierSolution @19:36:55.28 delay 2 seconds @19:36:57.31 AS.reconfigure ASconfig @19:36:57.36 AS.seek 1ml @19:37:26.39 delay 1 second @19:37:27.40 AFV.select :holdingCoil @19:37:27.51 delay 1 second @19:37:28.53 ATV.dial :waste @19:37:30.09 AS.reconfigure ASnorm @19:37:30.14 AS.seek :empty @19:38:20.88 AFV.select :carrierSolution @19:38:21.00 delay 2 seconds @19:38:23.03 AS.reconfigure ASconfig @19:38:23.08 AS.seek 0.5ml @19:38:52.17 delay 1 second @19:38:53.18 AFV.select :holdingCoil @19:38:53.29 Building Slug @19:38:53.30 ATV.dial :ARV @19:38:54.93 delay 2 seconds @19:38:56.95 ARV.dial :air1 @19:38:57.44 delay 2 seconds @19:38:59.52 AS.reconfigure ASslow2 @19:38:59.57 AS.seek 0.506ml @19:39:05.84 delay 2 seconds @19:39:07.85 ARV.dial :MM1 @19:39:08.45 delay 2 seconds @19:39:10.48 Adding MM1 @19:39:10.55 AS.seek 0.524ml @19:39:28.82 delay 25 seconds @19:39:53.84 ARV.dial :PP1 @19:39:54.23 Adding PP1 @19:39:54.24 delay 2 seconds @19:39:56.33 AS.seek 0.53ml @19:40:02.58 delay 10 seconds @19:40:12.61 ARV.dial :air1 @19:40:12.98 adding ARV air @19:40:12.99 delay 2 seconds @19:40:15.07 AS.seek 0.536ml @19:40:21.30 delay 5 seconds @19:40:26.33 ARV.dial :carrier @19:40:26.73 delay 7 seconds @19:40:33.81 AS.seek 0.594ml @19:41:32.53 delay 5 seconds @19:41:37.55 ATV.dial :ASV @19:41:38.53 delay 2 seconds @19:41:40.62 AS.seek 0.6ml @19:41:46.90 delay 4 seconds @19:41:50.92 ATV.dial ARV @19:41:51.45 delay 2 seconds @19:41:53.47 ARV.dial :carrier @19:41:53.49 delay 2 seconds @19:41:55.58 AS.seek 0.65ml @19:42:46.20 delay 4 seconds @19:42:50.22 ATV.dial PCR @19:42:50.81 ASV.dial :waste1 @19:42:50.82 ARV.dial :air1 @19:42:51.23 Positioning Slug by 0.324ml @19:42:51.32 AS.seek 0.326ml @19:48:17.34 delay 5 seconds @19:48:22.36 ATV.dial ARV @19:48:23.20 ASV.dial :PCRlock @19:48:23.58 delay 5 seconds @19:48:28.61 Running IPc ProfileIPc thermocycling @19:48:28.62 PCR.program ProfileIPc @19:48:28.78 ProfileIPc should complete in < 1:50:49 @19:48:28.79 PCR.run 2:23:31.25 @21:12:48.58 PCR.off @21:12:51.18 PCR for IPc completed -- closed /var/log/esp/IPc18oct1819h.pcr @21:12:51.19 Full MFB Decontamination @21:12:51.20 Decon extraction column and decon AIV @21:12:51.22 SPE.hold 2:46:40,55C @21:12:51.28 ASV.dial :SPEdelivery @21:12:51.90 delay 20 seconds @21:13:11.92 AFV.select :carrierSolution @21:13:12.03 delay 2 seconds @21:13:14.06 AS.reconfigure ASconfig @21:13:14.11 AS.seek 0.5ml @21:13:24.40 delay 1 second @21:13:25.41 AFV.select :holdingCoil @21:13:25.52 ATV.dial :air1 @21:13:27.49 AS.reconfigure ASslow1 @21:13:27.54 AS.seek 0.52ml @21:13:31.86 ATV.dial :ASV @21:13:33.37 ASV.dial :SPErecovery @21:13:34.02 AS.seek 0.62ml @21:13:54.38 ATV.dial :waste @21:13:56.28 AS.reconfigure ASnorm @21:13:56.33 AS.seek :empty @21:14:27.96 AFV.select :holdingCoil @21:14:27.98 ATV.dial :waste @21:14:28.00 AS.reconfigure ASconfig @21:14:28.04 AS.seek 0ml @21:14:28.38 AFV.select :carrierSolution @21:14:28.50 delay 2 seconds @21:14:30.53 AS.seek 0.5ml @21:14:59.56 delay 1 second @21:15:00.57 AFV.select :holdingCoil @21:15:00.68 ATV.dial :air3 @21:15:01.47 AS.reconfigure ASslow1 @21:15:01.52 AS.seek 0.52ml @21:15:05.78 ATV.dial :air3 @21:15:05.87 AS.seek 0.53ml @21:15:08.15 delay 2 seconds @21:15:10.16 ATV.dial :bleach @21:15:11.19 AS.seek 0.71ml @21:15:47.65 delay 3 seconds @21:15:50.67 ATV.dial :air3 @21:15:51.23 delay 3 seconds @21:15:54.32 AS.seek 0.735ml @21:15:59.60 delay 3 seconds @21:16:02.62 ATV.dial :bleach @21:16:03.26 AS.seek 0.72ml @21:16:06.56 delay 3 seconds @21:16:09.59 ATV.dial ASV @21:16:10.61 ASV.dial :SPErecovery @21:16:10.70 AS.seek 0.365ml @21:17:22.36 ATV.dial :waste @21:17:24.05 AS.reconfigure ASnorm @21:17:24.10 AS.seek :empty @21:17:42.86 ATV.dial :air3 @21:17:43.54 AS.reconfigure ASslow1 @21:17:43.59 AS.seek 0.009ml @21:17:45.95 delay 2 seconds @21:17:47.97 ATV.dial :bleach @21:17:48.43 AS.seek 0.579ml @21:19:43.31 delay 3 seconds @21:19:46.33 ATV.dial :air3 @21:19:46.87 delay 3 seconds @21:19:49.96 AS.seek 0.604ml @21:19:55.26 delay 3 seconds @21:19:58.28 ATV.dial :bleach @21:19:58.90 AS.seek 0.589ml @21:20:02.16 delay 3 seconds @21:20:05.18 ATV.dial :ASV @21:20:06.17 ASV.dial :wasteR @21:20:06.55 AS.seek 0ml @21:22:05.31 delay 1:00 @21:23:05.33 ATV.dial ASV @21:23:05.35 AFV.select :carrierSolution @21:23:05.45 delay 2 seconds @21:23:07.48 AS.reconfigure ASconfig @21:23:07.53 AS.seek 1ml @21:24:05.28 delay 1 second @21:24:06.29 AFV.select :holdingCoil @21:24:06.40 delay 1 second @21:24:07.42 ASV.dial :wasteR @21:24:07.44 AS.reconfigure ASslow1 @21:24:07.50 AS.seek 0ml @21:27:28.92 delay 10 seconds @21:27:38.94 ATV.dial :air3 @21:27:40.38 AS.seek 0.01ml @21:27:42.70 delay 2 seconds @21:27:44.71 ATV.dial :bleach @21:27:45.34 AS.seek 0.58ml @21:29:40.30 delay 3 seconds @21:29:43.32 ATV.dial :air3 @21:29:43.85 delay 3 seconds @21:29:46.93 AS.seek 0.605ml @21:29:52.24 delay 3 seconds @21:29:55.25 ATV.dial :bleach @21:29:55.87 AS.seek 0.59ml @21:29:59.21 delay 3 seconds @21:30:02.24 ATV.dial ASV @21:30:03.24 ASV.dial :SPEdelivery @21:30:03.69 AS.seek 0ml @21:32:02.61 delay 1:00 @21:33:02.63 AFV.select :carrierSolution @21:33:02.74 delay 2 seconds @21:33:04.78 AS.reconfigure ASconfig @21:33:04.85 AS.seek 1ml @21:34:02.61 delay 1 second @21:34:03.62 AFV.select :holdingCoil @21:34:03.73 delay 1 second @21:34:04.75 ATV.dial ASV @21:34:04.77 ASV.dial :SPEdelivery @21:34:04.78 AS.reconfigure ASslow1 @21:34:04.83 AS.seek 0ml @21:37:26.24 delay 10 seconds @21:37:36.26 ATV.dial :air2 @21:37:37.05 AS.reconfigure ASslow0 @21:37:37.10 AS.seek 0.8ml @21:38:57.90 delay 3 seconds @21:39:00.92 ATV.dial :ASV @21:39:01.87 ASV.dial :SPEdelivery @21:39:01.90 AS.reconfigure ASslow1 @21:39:01.95 AS.seek 0.03ml @21:41:37.16 delay 20 seconds @21:41:57.19 ATV.dial :waste,via: :air3 @21:41:58.82 AS.reconfigure ASconfig @21:41:58.87 AS.seek :empty @21:42:00.93 SPE.coast @21:42:01.02 Removing Bleach from Mixing Coil @21:42:01.03 AFV.select :carrierSolution @21:42:01.13 delay 2 seconds @21:42:03.16 AS.seek 0.5ml @21:42:32.85 delay 1 second @21:42:33.86 AFV.select :holdingCoil @21:42:33.97 ATV.dial :ASV @21:42:35.68 ASV.dial :SPErecovery @21:42:36.31 AS.reconfigure ASslow1 @21:42:36.36 AS.seek 0.73ml @21:43:22.87 ASV.dial :wasteD @21:43:23.44 AS.reconfigure ASnorm @21:43:23.49 AS.seek 0ml @21:44:00.61 AFV.select :carrierSolution @21:44:00.73 delay 2 seconds @21:44:02.76 AS.reconfigure ASconfig @21:44:02.81 AS.seek 0.5ml @21:44:31.87 delay 1 second @21:44:32.88 AFV.select :holdingCoil @21:44:32.99 ATV.dial ASV @21:44:33.00 ASV.dial :SPErecovery @21:44:33.64 AS.reconfigure ASslow0 @21:44:33.69 AS.seek 0.3ml @21:44:54.12 ATV.dial :air2 @21:44:55.11 AS.reconfigure ASslow1 @21:44:55.16 AS.seek 0.32ml @21:44:59.49 ATV.dial ASV @21:45:00.50 AS.reconfigure ASslow0 @21:45:00.55 AS.seek 0.57ml @21:45:25.92 ASV.dial :wasteD @21:45:26.60 AS.reconfigure ASnorm @21:45:26.65 AS.seek 0ml @21:45:55.70 AFV.select :carrierSolution @21:45:55.82 delay 2 seconds @21:45:57.85 AS.reconfigure ASconfig @21:45:57.90 AS.seek 0.5ml @21:46:27.01 delay 1 second @21:46:28.02 AFV.select :holdingCoil @21:46:28.13 ATV.dial ASV @21:46:28.14 ASV.dial :SPErecovery @21:46:28.76 AS.reconfigure ASslow0 @21:46:28.81 AS.seek 0.3ml @21:46:49.20 ATV.dial :air2 @21:46:50.20 AS.reconfigure ASslow1 @21:46:50.25 AS.seek 0.32ml @21:46:54.63 ATV.dial ASV @21:46:55.61 AS.reconfigure ASslow0 @21:46:55.66 AS.seek 0.57ml @21:47:21.11 ASV.dial :wasteD @21:47:21.70 AS.reconfigure ASnorm @21:47:21.75 AS.seek 0ml @21:47:50.83 ATV.dial :air2 @21:47:51.77 AS.reconfigure ASslow0 @21:47:51.82 AS.seek 0.3ml @21:48:22.34 ATV.dial :ASV @21:48:23.26 ASV.dial :wasteD @21:48:23.29 AS.reconfigure ASconfig @21:48:23.33 AS.seek 0.05ml @21:48:38.12 ATV.dial :waste @21:48:39.87 AS.seek 0ml @21:48:43.03 ASV.dial :SPEdelivery @21:48:43.99 deconLysateINT @21:48:44.00 ATV.dial :air3 @21:48:44.66 AS.reconfigure ASslow1 @21:48:44.71 AS.seek 0.01ml @21:48:47.05 delay 2 seconds @21:48:49.06 ATV.dial :bleach @21:48:49.53 AS.seek 0.96ml @21:52:00.81 delay 3 seconds @21:52:03.83 ATV.dial :air3 @21:52:04.37 delay 3 seconds @21:52:07.45 AS.seek 0.985ml @21:52:12.73 delay 3 seconds @21:52:15.75 ATV.dial :bleach @21:52:16.34 AS.seek 0.97ml @21:52:19.65 delay 3 seconds @21:52:22.67 ATV.dial AIV @21:52:23.85 AIV.dial :coilToMFB @21:52:23.86 AWV.select :waste @21:52:23.87 AFV.select :carrierSolution @21:52:23.98 delay 2 seconds @21:52:26.01 AS.reconfigure ASconfig @21:52:26.06 AS.seek 1ml @21:52:28.09 delay 1 second @21:52:29.10 AFV.select :holdingCoil @21:52:29.21 delay 1 second @21:52:30.24 AS.reconfigure ASslow0 @21:52:30.28 AS.seek :empty @21:54:11.25 AFV.select :carrierSolution @21:54:11.37 delay 2 seconds @21:54:13.96 AS.reconfigure ASconfig @21:54:14.03 AS.seek 1ml @21:55:11.88 delay 1 second @21:55:12.89 AFV.select :holdingCoil @21:55:13.00 delay 1 second @21:55:14.03 AS.reconfigure ASslow0 @21:55:14.08 AS.seek :empty @21:56:55.06 AFV.select :carrierSolution @21:56:55.18 delay 2 seconds @21:56:57.21 AS.reconfigure ASconfig @21:56:57.26 AS.seek 1ml @21:57:55.06 delay 1 second @21:57:56.07 AFV.select :holdingCoil @21:57:56.18 delay 1 second @21:57:57.21 AS.reconfigure ASslow0 @21:57:57.26 AS.seek :empty @21:59:38.24 ATV.dial :air2 @21:59:39.00 AS.reconfigure ASslow1 @21:59:39.05 AS.seek 0.009ml @21:59:41.37 AS.reconfigure ASslow0 @21:59:41.42 AS.seek 1ml @22:01:21.36 ATV.dial AIV @22:01:22.18 AS.seek 0.02ml @22:03:01.06 ATV.dial :waste @22:03:02.71 AS.seek 0ml @22:03:05.02 AWV.select :air @22:03:06.01 Cleaning PCR @22:03:06.03 AFV.select :carrierSolution @22:03:06.14 delay 2 seconds @22:03:08.18 AS.reconfigure ASconfig @22:03:08.23 AS.seek 0.5ml @22:03:37.26 delay 1 second @22:03:38.27 AFV.select :holdingCoil @22:03:38.38 ASV.dial :waste1 @22:03:38.75 ATV.dial :air3 @22:03:39.40 AS.reconfigure ASslow1 @22:03:39.46 AS.seek 0.51ml @22:03:41.75 delay 2 seconds @22:03:43.76 ATV.dial :bleach @22:03:44.22 AS.seek 0.81ml @22:04:44.81 delay 3 seconds @22:04:47.83 ATV.dial :air3 @22:04:48.36 delay 3 seconds @22:04:51.45 AS.seek 0.835ml @22:04:56.76 delay 3 seconds @22:04:59.78 ATV.dial :bleach @22:05:00.38 AS.seek 0.82ml @22:05:03.64 delay 3 seconds @22:05:06.66 ARV.dial :waste @22:05:07.26 delay 3 seconds @22:05:10.28 ATV.dial :ARV @22:05:11.12 AS.reconfigure ASconfig @22:05:11.17 AS.seek 0.47ml @22:05:31.58 AFV.select :carrierSolution @22:05:31.70 delay 2 seconds @22:05:33.73 AS.seek 1ml @22:06:04.48 delay 1 second @22:06:05.49 AFV.select :holdingCoil @22:06:05.60 delay 1 second @22:06:06.63 AS.reconfigure ASfast @22:06:06.68 AS.seek 0ml @22:06:34.00 AFV.select :carrierSolution @22:06:34.12 delay 2 seconds @22:06:36.15 AS.reconfigure ASconfig @22:06:36.19 AS.seek 1ml @22:07:34.04 delay 1 second @22:07:35.05 AFV.select :holdingCoil @22:07:35.16 delay 1 second @22:07:36.19 AS.reconfigure ASfast @22:07:36.24 AS.seek 0ml @22:08:03.58 AFV.select :carrierSolution @22:08:03.70 delay 2 seconds @22:08:05.73 AS.reconfigure ASconfig @22:08:05.78 AS.seek 1ml @22:09:03.56 delay 1 second @22:09:04.57 AFV.select :holdingCoil @22:09:04.68 delay 1 second @22:09:05.71 AS.reconfigure ASfast @22:09:05.76 AS.seek 0ml @22:09:33.19 ARV.dial between carrier and air1 @22:09:33.78 ATV.dial :air3 @22:09:35.00 AS.reconfigure ASslow1 @22:09:35.06 AS.seek 0.01ml @22:09:37.40 delay 2 seconds @22:09:39.41 ATV.dial :bleach @22:09:40.02 AS.seek 0.81ml @22:12:21.15 delay 3 seconds @22:12:24.17 ATV.dial :air3 @22:12:24.70 delay 3 seconds @22:12:27.79 AS.seek 0.835ml @22:12:33.08 delay 3 seconds @22:12:36.10 ATV.dial :bleach @22:12:36.69 AS.seek 0.82ml @22:12:39.99 delay 3 seconds @22:12:43.01 ATV.dial PCR @22:12:43.98 AS.reconfigure ASconfig @22:12:44.05 AS.seek :empty @22:13:31.51 PCR.program :bleachProfile @22:13:31.58 PCR program should complete in < 2:45 @22:13:31.59 PCR.run 10:00 @22:16:51.59 PCR.off @22:16:51.62 AFV.select :carrierSolution @22:16:51.74 delay 2 seconds @22:16:53.77 AS.seek 1ml @22:17:51.61 delay 1 second @22:17:52.62 AFV.select :holdingCoil @22:17:52.73 delay 1 second @22:17:53.76 AS.reconfigure ASfast @22:17:53.81 AS.seek 0ml @22:18:21.16 delay 3 seconds @22:18:24.18 AFV.select :carrierSolution @22:18:24.29 delay 2 seconds @22:18:26.32 AS.reconfigure ASconfig @22:18:26.38 AS.seek 1ml @22:19:24.14 delay 1 second @22:19:25.15 AFV.select :holdingCoil @22:19:25.26 delay 1 second @22:19:26.29 AS.reconfigure ASfast @22:19:26.34 AS.seek 0ml @22:19:53.67 delay 3 seconds @22:19:56.69 AFV.select :carrierSolution @22:19:56.80 delay 2 seconds @22:19:58.83 AS.reconfigure ASconfig @22:19:58.89 AS.seek 1ml @22:20:56.67 delay 1 second @22:20:57.68 AFV.select :holdingCoil @22:20:57.79 delay 1 second @22:20:58.82 AS.reconfigure ASfast @22:20:58.87 AS.seek 0ml @22:21:26.21 delay 3 seconds @22:21:29.23 AFV.select :carrierSolution @22:21:29.34 delay 2 seconds @22:21:31.37 AS.reconfigure ASconfig @22:21:31.43 AS.seek 1ml @22:22:29.21 delay 1 second @22:22:30.22 AFV.select :holdingCoil @22:22:30.33 delay 1 second @22:22:31.36 AS.reconfigure ASfast @22:22:31.41 AS.seek 0ml @22:22:58.74 delay 3 seconds @22:23:01.76 AFV.select :carrierSolution @22:23:01.87 delay 2 seconds @22:23:03.90 AS.reconfigure ASconfig @22:23:03.96 AS.seek 1ml @22:24:01.75 delay 1 second @22:24:02.76 AFV.select :holdingCoil @22:24:02.87 delay 1 second @22:24:03.91 AS.reconfigure ASnorm @22:24:03.96 AS.seek 0ml @22:24:54.68 AFV.select :carrierSolution @22:24:54.79 delay 2 seconds @22:24:56.82 AS.reconfigure ASconfig @22:24:56.86 AS.seek 1ml @22:25:54.63 delay 1 second @22:25:55.64 AFV.select :holdingCoil @22:25:55.75 delay 1 second @22:25:56.77 ATV.dial :waste @22:25:57.93 AS.reconfigure ASnorm @22:25:57.98 AS.seek 0.5ml @22:26:23.52 ATV.dial :waste2 @22:26:24.72 AS.seek 0ml @22:26:50.24 AS.coast @22:26:50.28 SS.coast @22:26:50.32 CC.coast @22:26:50.36 CS.coast @22:26:50.40 PC.coast @22:26:50.44 PS.coast @22:26:50.48 Forearm.coast @22:26:50.52 Elbow.coast @22:26:50.91 Gate.power :core,:OFF @22:26:50.92 Gate.power :raw,:OFF @22:26:50.93 Shutdown @22:26:50.95 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @14:00:00.05CEST19-Oct-18
Powering core bus @14:00:04.80 Gate.power :raw,:ON @14:00:05.44 SPE.coast @14:00:05.50 SPE.configure SPEconfig @14:00:05.60 AS.configure ASconfig @14:00:05.71 SS.configure SSconfig @14:00:05.80 CC.configure CCconfig @14:00:05.90 CS.configure CSconfig @14:00:06.00 PC.configure PCconfig @14:00:06.10 PS.configure PSconfig @14:00:06.22 Forearm.configure LooseForearm @14:00:06.32 Elbow.configure LooseElbow @14:00:06.44 SC.configure SCloose @14:00:06.54 SE.configure SEconfig @14:00:06.59 CH.coast @14:00:06.65 CH.configure CHconfig @14:00:06.68 PH.coast @14:00:06.74 PH.configure PHconfig @14:00:06.78 PCR.off @14:00:06.81 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:06.84 PCR.caltemp= 2.7073,0.0373,0 @14:00:06.86 All dwarf controllers running firmware version 3.75 @14:00:06.98 Valve.reset @14:00:07.15 PCR.off @14:00:07.18 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:07.21 PCR.caltemp= 2.7073,0.0373,0 @14:00:07.44 PSV.dial :air @14:00:07.47 Intake.select :close @14:00:07.50 Exhaust.select :close @14:00:07.53 CTV.dial :air @14:00:07.65 CSV.dial :waste1 @14:00:07.75
Forearm.reconfigure LooseForearm @14:00:07.80 Forearm.home.seek :retract @14:00:07.86 SSV.dial :spit,avoiding: :exhaust @14:00:07.98 PS.reconfigure PSconfig @14:00:08.03 PS.home.seek 0ml @14:00:08.54 Hand.close @14:00:08.57
Elbow.jog 1500 @14:00:08.74 CS.reconfigure CSfast @14:00:08.77 SS.reconfigure SSconfig @14:00:08.87 CS.home.seek 0ml @14:00:08.90 SS.home.seek 0ml @14:00:09.18
Elbow.reconfigure LooseElbow @14:00:09.23 Elbow.home.seek :clear @14:00:12.62 SE.reconfigure SEconfig @14:00:12.67 SE.home.seek :down @14:00:13.40 SC.reconfigure SCsloppy @14:00:13.44 SC.home.seek :initial @14:00:17.10 PS.coast @14:00:18.96
Forearm.seek :retract @14:00:19.04 Elbow.seek :hammer @14:00:20.40 Forearm.reconfigure TightForearm @14:00:20.45 Forearm.seek 5 + 350 counts @14:00:21.53 Forearm.seek :retract @14:00:22.72 PC.reconfigure PCconfig @14:00:22.73
CC.reconfigure CCconfig @14:00:22.83 PC.home.seek :open @14:00:22.85
CC.home.seek :open @14:00:26.79 Phase [mbaPhase] @14:00:26.80 GoodNews.email "At 14:00:26 began in tube 4: mbaPhase\n/home/esp/esp2/mission/18tundeployv4.rb:122\n{1 => 22, 2 => 22, 3 => 1, 4 => 21}\nCan@14:00:26, 13.4C, 72% humidity, 14.3psia, 12.925V, 0.714A, 0.714A avg, 9.23W",Subject: "Started 18tundeployv4 phase mbaPhase" @14:00:26.83CEST19-Oct-18
==================> STARTED Superphase (phase #2) <=== @14:00:26.86 Starting initialPurge @14:00:26.87 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:00:26.95 Hand.open @14:00:27.83 Elbow.reconfigure TightElbow @14:00:27.88 Elbow.seek FlushPuck::Garage @14:00:29.77 Elbow.reconfigure LooseElbow @14:00:29.88 Forearm.reconfigure LooseForearm @14:00:29.95 Forearm.seek :garage @14:00:30.93 Hand.close @14:00:31.85 Forearm.seek :retract @14:00:32.85 Elbow.reconfigure TightElbow @14:00:32.90 Elbow.seek CC @14:00:35.88 Elbow.reconfigure LooseElbow @14:00:36.00 Forearm.reconfigure TightForearm @14:00:36.06 Forearm.seek CC @14:00:37.37 CC.seek :closed @14:00:41.64 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.15 @14:00:41.72 Hand.open @14:00:42.51 Forearm.reconfigure LooseForearm @14:00:42.56 Forearm.seek :retract @14:00:43.54 Flushing the Collection Valve::Manifold @14:00:43.67 CSV.dial CSR @14:00:45.13 Pulling 5ml of CSR.flush @14:00:45.14 CSR.select :flush @14:00:45.26 CS.reconfigure CSconfig @14:00:45.30 CS.seek 5ml @14:00:59.15 delay 5 seconds @14:01:04.17 CSR.select :air @14:01:04.34 CS.reconfigure CSfast @14:01:04.40 CS.seek 7.501ml @14:01:11.58 CSV.dial :waste @14:01:12.98 CS.reconfigure CSconfig @14:01:13.03 CS.seek 0ml @14:01:33.15 Purging external Intake @14:01:33.28 CSV.dial :flush @14:01:33.78 CS.reconfigure CSfast @14:01:33.83 CS.seek 10ml @14:01:58.90 delay 5 seconds @14:02:03.93 CSV.dial CBV @14:02:03.96
CBV.dial CSV @14:02:04.78 CTV.dial :intake @14:02:05.60 Intake.select :open @14:02:05.73 CS.seek 0ml @14:02:31.77 Intake.select :close @14:02:31.89 CTV.dial :air @14:02:32.70 Flushing upper manifold @14:02:32.83 CSV.dial :flush @14:02:33.62 CS.seek 5ml @14:02:46.72 delay 5 seconds @14:02:51.73 CSV.dial :air @14:02:52.80 CS.seek 7ml @14:02:58.56 CSV.dial CBV @14:02:58.58
CBV.dial CSV @14:02:59.70 CTV.dial :air @14:02:59.72 CS.seek 0ml @14:03:17.37 delay 7 seconds @14:03:24.40 CS.seek 3ml @14:03:32.52 CSV.dial :waste @14:03:33.02 CS.seek 0ml @14:03:41.40 CSV.dial :flush @14:03:41.89 CS.seek 3ml @14:03:50.15 delay 5 seconds @14:03:55.17 CSV.dial :air @14:03:55.74 CS.seek 5ml @14:04:01.46 CSV.dial :air @14:04:01.49 CS.seek 0ml @14:04:14.47 delay 7 seconds @14:04:21.50 CS.seek 3ml @14:04:29.71 CSV.dial :waste @14:04:30.53 CS.seek 0ml @14:04:38.66 Garaging FlushPuck in Collection Clamp @14:04:38.74 Elbow.reconfigure TightElbow @14:04:38.79 Elbow.seek CC @14:04:39.06 Elbow.reconfigure LooseElbow @14:04:39.17 Forearm.seek CC @14:04:40.16 Hand.close @14:04:40.95 CC.seek :open @14:04:45.34 Forearm.seek :retract @14:04:46.36 Elbow.reconfigure TightElbow @14:04:46.41 Elbow.seek :garage @14:04:49.42 Elbow.reconfigure LooseElbow @14:04:49.52 Forearm.reconfigure TightForearm @14:04:49.57 Forearm.seek :garage @14:04:50.73 Hand.open @14:04:51.52 Forearm.reconfigure LooseForearm @14:04:51.56 Forearm.seek :retract @14:04:52.70 Starting mfb @14:04:52.71 pcrBAC sampling at most 300ml, wcr at most 1500ml @14:04:52.74 BACfans.email "Started mfb in tube 4 at 14:04:52CEST19-Oct-18\n{1 => 22, 2 => 22, 3 => 1, 4 => 21}\nCan@14:04:19, 13.4C, 72% humidity, 14.3psia, 12.842V, 0.966A, 0.966A avg, 12.41W",Subject: "pcrBAC sampling at most 300ml, wcr at most 1500ml" @14:04:52.83
Loading sh1 puck from tube 4 into Collection Clamp @14:04:52.93 Readying the MFB @14:04:52.97 Homing MFB Syringe @14:04:53.01 AFV.select :holdingCoil @14:04:53.24 ATV.dial :air2 @14:04:53.27
SC.reconfigure SCtight @14:04:53.36 SC.seek 4 @14:04:53.84 AS.reconfigure ASconfig @14:04:53.89 AS.home.seek :home @14:04:56.97
SC.reconfigure SCloose @14:04:57.21 Elbow.reconfigure TightElbow @14:04:57.26 Elbow.seek 4 @14:04:59.40 AFV.select :carrierSolution @14:04:59.45
Forearm.reconfigure TightForearm @14:04:59.53 Forearm.seek 4 + 700 counts @14:04:59.61 delay 2 seconds @14:05:00.71
SE.reconfigure SEpick @14:05:00.78 SE.seek beamBreak @14:05:01.64 AS.seek 1ml @14:05:01.92
SE.configure SEconfig @14:05:02.14 Elbow.seek 4 @14:05:02.37 Forearm.seek 4 + 300 counts @14:05:02.64 Forearm.reconfigure LooseForearm @14:05:02.69 Hand.close @14:05:03.47 SE.seek :down @14:05:03.71
Forearm.seek :retract @14:05:04.45 SE.coast @14:05:04.69
Elbow.seek CC @14:05:06.40 Elbow.reconfigure LooseElbow @14:05:06.50 Forearm.reconfigure TightForearm @14:05:06.55 Forearm.seek CC @14:05:07.88 CC.seek :closed @14:05:12.15 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.20 @14:05:12.16 Hand.open @14:05:12.91 Forearm.reconfigure LooseForearm @14:05:12.95 Forearm.seek :retract @14:05:13.91 SC.coast @14:05:13.96 collectSample 300ml w/bubble point of 55psi @14:05:13.97 Priming 7ml @14:05:13.98 Intake.select :close @14:05:13.99 Exhaust.select :close @14:05:14.00 SS.hold @14:05:14.25 Calibrating pressure and temperature sensors @14:05:14.27 CTV.dial :air @14:05:14.28 SSV.dial :spit,avoiding: :exhaust @14:05:14.30 SS.reconfigure SSfast @14:05:14.35 SS.seek 5ml @14:05:17.06 SSV.dial CBV @14:05:17.09
CBV.dial SSV @14:05:18.55 delay 3.8 seconds @14:05:22.49 SSV.dial :spit @14:05:23.93 SS.seek 0ml @14:05:26.60 SSV.dial CBV @14:05:28.00 CTV.dial :intake @14:05:28.81 SSV.dial CBV @14:05:28.84
CBV.dial SSV @14:05:28.85 Allowing 40s for 7.0ml of remaining 7.0ml @14:05:28.86 Intake.select :open @14:05:31.04 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:05:31.06 SS.reconfigure SSinhale.with maxSpeed: 150 @14:05:31.11 SS.seek 7ml @14:05:34.63 Vacuum too high -- waiting up to 40s @14:05:35.95 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:05:35.97 SS.reconfigure SSinhale.with maxSpeed: 135 @14:05:36.03 SS.seek 7ml @14:05:40.85 Stroke completed -- waiting for pressure to equalize ... @14:05:43.48 Intake.select :close @14:05:43.64 SSV.dial :exhaust @14:05:44.47 Exhaust.select :open @14:05:44.58 SS.reconfigure SSexhale @14:05:44.63 SS.seek 0ml @14:05:48.14 Exhaust.select :close @14:05:48.26 Sampling 300.0ml @14:05:48.27 Intake.select :close @14:05:48.28 Exhaust.select :close @14:05:48.30 SS.reconfigure SSconfig @14:05:48.34 SS.hold @14:05:48.57 CTV.dial :intake @14:05:48.59 SSV.dial CBV @14:05:48.61
CBV.dial SSV @14:05:49.45 Allowing 130s for 25.0ml of remaining 300.0ml @14:05:49.46 Intake.select :open @14:05:51.63 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:05:51.65 SS.reconfigure SSinhale.with maxSpeed: 135 @14:05:51.70 SS.seek 25ml @14:05:54.51 delay 1 second @14:05:55.52 AFV.select :holdingCoil @14:05:55.63 delay 1 second @14:05:55.89
Vacuum too high -- waiting up to 40s @14:05:56.67 ATV.dial :waste @14:05:57.22
Intake @32.9psia, vacuum from 20.0 to 24.0psi @14:05:57.24 SS.reconfigure SSinhale.with maxSpeed: 122 @14:05:57.29 SS.seek 25ml @14:05:57.70 AS.reconfigure ASnorm @14:05:57.75 AS.home.seek :empty @14:06:00.14
Vacuum too high -- waiting up to 40s @14:06:01.49 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:06:01.51 SS.reconfigure SSinhale.with maxSpeed: 110 @14:06:01.56 SS.seek 25ml @14:06:04.26 Vacuum too high -- waiting up to 40s @14:06:05.59 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:06:05.61 SS.reconfigure SSinhale.with maxSpeed: 99 @14:06:05.66 SS.seek 25ml @14:06:08.70 Vacuum too high -- waiting up to 40s @14:06:09.78 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:06:09.80 SS.reconfigure SSinhale.with maxSpeed: 89 @14:06:09.85 SS.seek 25ml @14:06:12.92 Vacuum too high -- waiting up to 40s @14:06:14.01 SS.reconfigure SSinhale.with maxSpeed: 80 @14:06:14.11 SS.seek 25ml @14:06:18.07 Vacuum too high -- waiting up to 40s @14:06:19.68 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:06:19.70 SS.reconfigure SSinhale.with maxSpeed: 72 @14:06:19.75 SS.seek 25ml @14:06:24.82 Vacuum too high -- waiting up to 40s @14:06:25.93 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:06:25.95 SS.reconfigure SSinhale.with maxSpeed: 65 @14:06:26.00 SS.seek 25ml @14:06:26.85 Stroke completed -- waiting for pressure to equalize ... @14:06:28.19 Intake.select :close @14:06:28.35 SSV.dial :exhaust @14:06:29.17 Exhaust.select :open @14:06:29.28 SS.reconfigure SSexhale @14:06:29.33 SS.seek 0ml @14:06:38.16 Exhaust.select :close @14:06:38.28 SSV.dial CBV @14:06:38.33
CBV.dial SSV @14:06:39.12 Allowing 130s for 25.0ml of remaining 275.0ml @14:06:39.13 Intake.select :open @14:06:41.30 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:06:41.32 SS.reconfigure SSinhale.with maxSpeed: 65 @14:06:41.37 SS.seek 25ml @14:06:49.32 Vacuum too high -- waiting up to 40s @14:06:50.14 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:06:50.16 SS.reconfigure SSinhale.with maxSpeed: 59 @14:06:50.21 SS.seek 25ml @14:06:50.32 ATV.dial :waste @14:06:50.34 ARV.dial between carrier and air1 @14:06:50.43 ASV.dial :waste1 @14:07:13.19
Stroke completed -- waiting for pressure to equalize ... @14:07:15.76 Intake.select :close @14:07:15.91 SSV.dial :exhaust @14:07:16.72 Exhaust.select :open @14:07:16.84 SS.reconfigure SSexhale @14:07:16.90 SS.seek 0ml @14:07:25.63 Exhaust.select :close @14:07:25.75 SSV.dial CBV @14:07:25.78
CBV.dial SSV @14:07:26.62 Allowing 130s for 25.0ml of remaining 250.0ml @14:07:26.63 Intake.select :open @14:07:28.80 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:07:28.82 SS.reconfigure SSinhale.with maxSpeed: 59 @14:07:28.87 SS.seek 25ml @14:07:44.26 Vacuum too high -- waiting up to 40s @14:07:45.09 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:07:45.11 SS.reconfigure SSinhale.with maxSpeed: 53 @14:07:45.16 SS.seek 25ml @14:08:02.93 Stroke completed -- waiting for pressure to equalize ... @14:08:05.49 Intake.select :close @14:08:05.64 SSV.dial :exhaust @14:08:06.47 Exhaust.select :open @14:08:06.59 SS.reconfigure SSexhale @14:08:06.65 SS.seek 0ml @14:08:15.69 Exhaust.select :close @14:08:15.81 SSV.dial CBV @14:08:15.84
CBV.dial SSV @14:08:16.69 Allowing 130s for 25.0ml of remaining 225.0ml @14:08:16.70 Intake.select :open @14:08:18.87 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:08:18.89 SS.reconfigure SSinhale.with maxSpeed: 53 @14:08:18.93 SS.seek 25ml @14:08:52.91 Stroke completed -- waiting for pressure to equalize ... @14:08:55.37 Intake.select :close @14:08:55.53 SSV.dial :exhaust @14:08:56.37 Exhaust.select :open @14:08:56.48 SS.reconfigure SSexhale @14:08:56.53 SS.seek 0ml @14:09:05.49 Exhaust.select :close @14:09:05.61 SSV.dial CBV @14:09:05.64
CBV.dial SSV @14:09:06.48 Allowing 130s for 25.0ml of remaining 200.0ml @14:09:06.49 Intake.select :open @14:09:08.66 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:09:08.68 SS.reconfigure SSinhale.with maxSpeed: 53 @14:09:08.73 SS.seek 25ml @14:09:42.64 Stroke completed -- waiting for pressure to equalize ... @14:09:45.23 Intake.select :close @14:09:45.38 SSV.dial :exhaust @14:09:46.22 Exhaust.select :open @14:09:46.34 SS.reconfigure SSexhale @14:09:46.39 SS.seek 0ml @14:09:55.19 Exhaust.select :close @14:09:55.31 SSV.dial CBV @14:09:55.34
CBV.dial SSV @14:09:56.19 Allowing 130s for 25.0ml of remaining 175.0ml @14:09:56.20 Intake.select :open @14:09:58.37 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:09:58.39 SS.reconfigure SSinhale.with maxSpeed: 53 @14:09:58.44 SS.seek 25ml @14:10:32.74 Stroke completed -- waiting for pressure to equalize ... @14:10:35.32 Intake.select :close @14:10:35.47 SSV.dial :exhaust @14:10:36.32 Exhaust.select :open @14:10:36.43 SS.reconfigure SSexhale @14:10:36.48 SS.seek 0ml @14:10:45.63 Exhaust.select :close @14:10:45.75 SSV.dial CBV @14:10:45.78
CBV.dial SSV @14:10:46.61 Allowing 130s for 25.0ml of remaining 150.0ml @14:10:46.62 Intake.select :open @14:10:48.79 Intake @33.7psia, vacuum from 20.0 to 24.0psi @14:10:48.81 SS.reconfigure SSinhale.with maxSpeed: 53 @14:10:48.85 SS.seek 25ml @14:11:22.69 Stroke completed -- waiting for pressure to equalize ... @14:11:25.25 Intake.select :close @14:11:25.40 SSV.dial :exhaust @14:11:26.23 Exhaust.select :open @14:11:26.34 SS.reconfigure SSexhale @14:11:26.40 SS.seek 0ml @14:11:35.57 Exhaust.select :close @14:11:35.69 SSV.dial CBV @14:11:35.72
CBV.dial SSV @14:11:36.56 Allowing 130s for 25.0ml of remaining 125.0ml @14:11:36.57 Intake.select :open @14:11:38.74 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:11:38.76 SS.reconfigure SSinhale.with maxSpeed: 53 @14:11:38.81 SS.seek 25ml @14:12:05.54 Vacuum too high -- waiting up to 40s @14:12:06.36 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:12:06.38 SS.reconfigure SSinhale.with maxSpeed: 48 @14:12:06.42 SS.seek 25ml @14:12:14.63 Stroke completed -- waiting for pressure to equalize ... @14:12:16.60 Intake.select :close @14:12:17.27 SSV.dial :exhaust @14:12:18.11 Exhaust.select :open @14:12:18.22 SS.reconfigure SSexhale @14:12:18.27 SS.seek 0ml @14:12:27.32 Exhaust.select :close @14:12:27.44 SSV.dial CBV @14:12:27.47
CBV.dial SSV @14:12:28.33 Allowing 130s for 25.0ml of remaining 100.0ml @14:12:28.34 Intake.select :open @14:12:30.52 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:12:30.53 SS.reconfigure SSinhale.with maxSpeed: 48 @14:12:30.58 SS.seek 25ml @14:13:08.02 Stroke completed -- waiting for pressure to equalize ... @14:13:10.61 Intake.select :close @14:13:10.77 SSV.dial :exhaust @14:13:11.60 Exhaust.select :open @14:13:11.72 SS.reconfigure SSexhale @14:13:11.78 SS.seek 0ml @14:13:20.83 Exhaust.select :close @14:13:20.95 SSV.dial CBV @14:13:20.98
CBV.dial SSV @14:13:21.81 Allowing 130s for 25.0ml of remaining 75.0ml @14:13:21.82 Intake.select :open @14:13:23.99 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:13:24.01 SS.reconfigure SSinhale.with maxSpeed: 48 @14:13:24.06 SS.seek 25ml @14:14:01.47 Stroke completed -- waiting for pressure to equalize ... @14:14:04.04 Intake.select :close @14:14:04.19 SSV.dial :exhaust @14:14:05.02 Exhaust.select :open @14:14:05.14 SS.reconfigure SSexhale @14:14:05.20 SS.seek 0ml @14:14:14.22 Exhaust.select :close @14:14:14.34 SSV.dial CBV @14:14:14.37
CBV.dial SSV @14:14:15.22 Allowing 130s for 25.0ml of remaining 50.0ml @14:14:15.23 Intake.select :open @14:14:17.40 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:14:17.42 SS.reconfigure SSinhale.with maxSpeed: 48 @14:14:17.46 SS.seek 25ml @14:14:55.13 Stroke completed -- waiting for pressure to equalize ... @14:14:57.71 Intake.select :close @14:14:57.87 SSV.dial :exhaust @14:14:58.69 Exhaust.select :open @14:14:58.81 SS.reconfigure SSexhale @14:14:58.86 SS.seek 0ml @14:15:08.02 Exhaust.select :close @14:15:08.14 SSV.dial CBV @14:15:08.17
CBV.dial SSV @14:15:09.00 Allowing 130s for 25.0ml of remaining 25.0ml @14:15:09.01 Intake.select :open @14:15:11.18 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:15:11.20 SS.reconfigure SSinhale.with maxSpeed: 48 @14:15:11.24 SS.seek 25ml @14:15:48.38 Stroke completed -- waiting for pressure to equalize ... @14:15:50.95 Intake.select :close @14:15:51.11 SSV.dial :exhaust @14:15:51.93 Exhaust.select :open @14:15:52.05 SS.reconfigure SSexhale @14:15:52.10 SS.seek 0ml @14:16:00.97 Exhaust.select :close @14:16:01.09 Sampled 300.0ml @14:16:01.10 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @14:16:01.11 Intake.select :close @14:16:01.18 CSV.dial :waste @14:16:01.24 CS.seek 0ml @14:16:01.35 CSV.dial :air @14:16:01.89 SSV.dial CBV,avoiding: :exhaust @14:16:01.93
CBV.dial SSV @14:16:02.74 CS.seek 10ml @14:16:03.98
CTV.dial :air @14:16:04.79 delay 15 seconds @14:16:19.82 SSV.dial :spit,avoiding: :exhaust @14:16:21.23 CTV.dial CSV,avoiding: :intake @14:16:21.28
SS.seek 0ml @14:16:21.41 SSV.dial CBV,avoiding: :exhaust @14:16:28.07 CBV.dial between SSV and waste2 @14:16:28.50 CTV.dial CSV,avoiding: :air @14:16:28.53
CSV.dial CTV @14:16:29.03 CS.reconfigure CSpressurize @14:16:29.08 CS.seek 0ml @14:16:55.19 BubblePoint: Intake @70.1psia, Outlet @14.7psia CBV.dial SSV @14:16:55.54 SS.reconfigure SSconfig @14:16:55.59 SS.seek 10ml @14:17:01.40 delay 15 seconds @14:17:16.53 Evacuated: Intake @35.7psia, Outlet @1.5psia @14:17:16.54 SSV.dial :spit,avoiding: :exhaust @14:17:17.98 SS.reconfigure SSexhale @14:17:18.03 SS.seek 0ml @14:17:23.46 SS.reconfigure SSfast @14:17:23.51 SS.seek 5ml @14:17:26.25 SSV.dial CBV,avoiding: :exhaust @14:17:26.28
CBV.dial SSV @14:17:27.72 CTV.dial :air,avoiding: :intake @14:17:29.15 CSV.dial CTV @14:17:29.20
SS.seek 10ml @14:17:30.19 CSV.dial :air @14:17:30.68 CS.reconfigure CSfast @14:17:30.73 CS.seek 0ml @14:17:31.25 CS.coast @14:17:31.89
SSV.dial :spit,avoiding: :exhaust @14:17:33.33 SS.reconfigure SSexhale @14:17:33.38 SS.seek 0ml @14:17:38.59 SS.reconfigure SSfast @14:17:38.65 SS.seek 5ml @14:17:41.15 SSV.dial CBV,avoiding: :exhaust @14:17:41.18
CBV.dial SSV @14:17:43.63 SSV.dial :spit,avoiding: :exhaust @14:17:45.08 SS.reconfigure SSconfig @14:17:45.13 SS.seek 0ml @14:17:48.27 SS.coast @14:17:48.32 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @14:17:48.33 Intake.select :close @14:17:48.41 CSV.dial :waste @14:17:48.54 SSV.dial CBV,avoiding: :exhaust @14:17:48.61
CBV.dial SSV @14:17:49.26 CS.seek 0ml @14:17:49.35 CSV.dial :air @14:17:50.00
CTV.dial :air @14:17:50.01 delay 15 seconds @14:17:50.15 CS.seek 10ml @14:18:05.04
SSV.dial :spit,avoiding: :exhaust @14:18:06.45 CTV.dial CSV,avoiding: :intake @14:18:06.50
SS.reconfigure SSexhale @14:18:06.59 SS.seek 0ml @14:18:06.66 SSV.dial CBV,avoiding: :exhaust @14:18:15.20 CBV.dial between SSV and waste2 @14:18:16.14 CSV.dial between air and CTV @14:18:16.60 CS.seek 8.263ml @14:18:21.73 CTV.dial CSV,avoiding: :air @14:18:21.76
CSV.dial CTV @14:18:22.11 CS.reconfigure CSpressurize @14:18:22.16 CS.seek 0ml @14:18:43.78 CSV.dial :air @14:18:44.28 CS.reconfigure CSfast @14:18:44.33 CS.seek 10ml @14:19:09.13 CSV.dial between air and CTV @14:19:09.49 CS.seek 3.138ml @14:19:26.92 CTV.dial CSV,avoiding: :air @14:19:26.95
CSV.dial CTV @14:19:27.28 CS.reconfigure CSpressurize @14:19:27.33 CS.seek 0ml @14:19:35.69 BubblePoint: Intake @69.4psia, Outlet @14.1psia @14:19:35.70 CBV.dial SSV @14:19:36.06 SS.reconfigure SSconfig @14:19:36.11 SS.seek 10ml @14:19:41.69 delay 15 seconds @14:19:56.83 Evacuated: Intake @39.6psia, Outlet @15.8psia @14:19:56.84 SSV.dial :spit,avoiding: :exhaust @14:19:58.26 SS.reconfigure SSexhale @14:19:58.31 SS.seek 0ml @14:20:03.32 SS.reconfigure SSfast @14:20:03.37 SS.seek 5ml @14:20:06.08 SSV.dial CBV,avoiding: :exhaust @14:20:06.11
CBV.dial SSV @14:20:07.56 CTV.dial :air,avoiding: :intake @14:20:08.98 CSV.dial CTV @14:20:09.02
SS.seek 10ml @14:20:10.02 CSV.dial :air @14:20:10.51 CS.reconfigure CSfast @14:20:10.56 CS.seek 0ml @14:20:11.69 CS.coast @14:20:11.76
SSV.dial :spit,avoiding: :exhaust @14:20:13.19 SS.reconfigure SSexhale @14:20:13.25 SS.seek 0ml @14:20:18.24 SS.reconfigure SSfast @14:20:18.29 SS.seek 5ml @14:20:20.97 SSV.dial CBV,avoiding: :exhaust @14:20:21.00
CBV.dial SSV @14:20:23.45 SSV.dial :spit,avoiding: :exhaust @14:20:24.87 SS.reconfigure SSconfig @14:20:24.92 SS.seek 0ml @14:20:28.05 SS.coast @14:20:28.09 Lysing: Adding 1.5ml lysis @14:20:28.11 CBV.dial between CSV and waste1,avoiding: :DBV @14:20:29.72 CTV.dial CSV @14:20:31.13 CSV.dial CSR @14:20:32.00 Pulling 1ml of CSR.lysis @14:20:32.01 CSR.select :lysis @14:20:32.12 CS.reconfigure CSconfig @14:20:32.18 CS.seek 1ml @14:20:35.70 delay 5 seconds @14:20:40.72 CSR.select :air @14:20:40.89 CS.seek 6ml @14:20:54.63 delay 2 seconds @14:20:56.65 CSV.dial :waste1 @14:20:58.06 CS.reconfigure CSfast @14:20:58.11 CS.seek :empty @14:21:13.35 CSV.dial CSR @14:21:15.29 Pulling 1.5ml of CSR.lysis @14:21:15.30 CSR.select :lysis @14:21:15.41 CS.reconfigure CSconfig @14:21:15.47 CS.seek 1.5ml @14:21:20.30 delay 5 seconds @14:21:25.32 CSR.select :air @14:21:25.51 CS.seek 4.5ml @14:21:34.14 delay 2 seconds @14:21:36.15 CSV.dial CTV @14:21:36.66 CS.seek 1.275ml @14:21:45.86 Heating sh1 puck from tube 4 in Collection Clamp to 85C for 5:00 @14:21:45.88 CH.seek 85C,8:00 @14:24:49.05 CH.hold 5:00,85C @14:29:49.22 Collection Puck Heater at 85.1C after lysing @14:29:49.24 CS.seek 0ml @14:29:53.46 CTV.dial between spare and air,avoiding: :intake @14:29:54.60 CSV.dial :air @14:29:55.09 CS.seek 5ml @14:30:08.89 CSV.dial CTV @14:30:09.40 delay 2 seconds @14:30:11.41 CSV.dial :air @14:30:11.91 delay 2 seconds @14:30:13.92 CSV.dial CTV @14:30:14.43 delay 2 seconds @14:30:16.44 CSV.dial :air @14:30:16.94 delay 2 seconds @14:30:18.96 CS.seek 0ml @14:30:32.74 Recovering Lysate into Collection syringe @14:30:32.75 CSV.dial CBV @14:30:33.83 CBV.dial CSV @14:30:34.25 CS.seek 5ml @14:30:47.96 delay 5 seconds @14:30:52.98 CTV.dial :air @14:30:53.56 CS.seek 7ml @14:30:59.68 delay 5 seconds @14:31:04.70 CSV.dial :air2 @14:31:05.73 CS.seek 10ml @14:31:14.34 CSV.dial between air and CTV,avoiding: CBV @14:31:15.57 Lysing Complete @14:31:15.65 Storing sh1 puck in Collection Clamp to tube 3 @14:31:15.85 SC.reconfigure SCtight @14:31:15.89 SC.seek 3 @14:31:17.98 SC.reconfigure SCloose @14:31:18.21 Elbow.reconfigure TightElbow @14:31:18.26 Elbow.seek 3 @14:31:20.29 Forearm.reconfigure TightForearm @14:31:20.34 Forearm.seek 3 + 700 counts @14:31:21.42 SE.reconfigure SEpick @14:31:21.49 SE.seek beamBreak @14:31:24.48 SE.configure SEconfig @14:31:24.65 Elbow.reconfigure LimpElbow @14:31:24.71 Forearm.reconfigure LooseForearm @14:31:24.76 Forearm.seek 3 @14:31:25.02 Elbow.hold @14:31:25.09 Elbow.reconfigure TightElbow @14:31:25.20 SE.seek 0.06 Pucks below Top Plate @14:31:25.64 Forearm.force -20 @14:31:25.69 Forearm.seek 3 + 345 counts @14:31:26.04 Forearm.reconfigure TightForearm @14:31:26.08 Forearm.seek 3 + 700 counts @14:31:26.40 SE.seek 1.06 Pucks below Top Plate @14:31:27.07 Forearm.reconfigure LooseForearm @14:31:27.11 Forearm.seek :retract @14:31:28.04 Elbow.seek CC @14:31:29.66 Elbow.reconfigure LooseElbow @14:31:29.77 Forearm.seek CC @14:31:30.73 Hand.close @14:31:31.49 CC.seek :open @14:31:36.00 Forearm.seek :retract @14:31:36.98 Elbow.reconfigure TightElbow @14:31:37.03 Elbow.seek 3 @14:31:39.03 Elbow.reconfigure LimpElbow @14:31:39.08 Forearm.seek 3 @14:31:40.12 Elbow.hold @14:31:40.19 Elbow.reconfigure TightElbow @14:31:40.24 Hand.open @14:31:41.01 Forearm.force -20 @14:31:41.05 Forearm.seek 3 + 336 counts @14:31:41.37 Forearm.reconfigure TightForearm @14:31:41.42 Forearm.seek 3 + 700 counts @14:31:42.41 SE.seek :down @14:31:43.59
Forearm.reconfigure LooseForearm @14:31:43.64 Forearm.seek :retract @14:31:45.12 SE.coast @14:31:45.16
SC.coast @14:31:45.20 Tranferring lysate to PRV @14:31:45.21 CSV.dial :air @14:31:45.67 CS.seek 10ml @14:31:45.74 CSV.dial between air and CTV @14:31:46.55 AIV.dial :bypass @14:31:46.63 Beginning samplePassC @14:31:46.64 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:31:46.72 Hand.open @14:31:47.12 Elbow.seek FlushPuck::Garage @14:31:49.08 Elbow.reconfigure LooseElbow @14:31:49.19 Forearm.seek :garage @14:31:50.20 Hand.close @14:31:51.00 Forearm.seek :retract @14:31:52.02 Elbow.reconfigure TightElbow @14:31:52.07 Elbow.seek CC @14:31:54.99 Elbow.reconfigure LooseElbow @14:31:55.10 Forearm.reconfigure TightForearm @14:31:55.15 Forearm.seek CC @14:31:56.44 CC.seek :closed @14:32:00.69 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.15 @14:32:00.78 Hand.open @14:32:01.44 Forearm.reconfigure LooseForearm @14:32:01.48 Forearm.seek :retract @14:32:02.48 AWV.select :air @14:32:02.49 AIV.dial :bypass @14:32:02.51 PSV.dial PSR @14:32:03.99 Pulling 1.5ml of PSR.flush @14:32:04.00 PSR.select :flush @14:32:04.13 PS.seek 1.5ml @14:32:20.13 delay 5 seconds @14:32:25.15 PSV.dial :waste1 @14:32:25.63 PS.seek 0ml @14:32:41.71 PSV.dial PSR @14:32:42.20 PSR.select :air @14:32:42.33 PS.seek 2.5ml @14:33:08.54 PRV.dial CBV @14:33:08.57
CBV.dial PRV @14:33:09.45 PSV.dial :mixloop @14:33:10.54 CTV.dial :air,avoiding: :intake @14:33:10.55 PTV.dial :PRVmixing @14:33:10.63 PS.seek 0ml @14:33:36.81 PSV.dial :air @14:33:37.29 PS.seek 2.5ml @14:34:03.43 PSV.dial :mixloop @14:34:03.91 PS.seek 0ml @14:34:30.06 PSV.dial :air @14:34:31.07 PS.seek 2.5ml @14:34:57.18 PSV.dial :mixloop @14:34:57.66 PS.seek 0ml @14:35:23.82 Garaging FlushPuck in Collection Clamp @14:35:23.90 Elbow.reconfigure TightElbow @14:35:23.95 Elbow.seek CC @14:35:24.18 Elbow.reconfigure LooseElbow @14:35:24.29 Forearm.seek CC @14:35:25.27 Hand.close @14:35:25.87 CC.seek :open @14:35:30.25 Forearm.seek :retract @14:35:31.26 Elbow.reconfigure TightElbow @14:35:31.31 Elbow.seek :garage @14:35:34.50 Elbow.reconfigure LooseElbow @14:35:34.60 Forearm.reconfigure TightForearm @14:35:34.65 Forearm.seek :garage @14:35:35.87 Hand.open @14:35:36.57 Forearm.reconfigure LooseForearm @14:35:36.62 Forearm.seek :retract @14:35:37.69 Connecting PS to CS to transfer sample @14:35:37.70 CBV.dial AIV @14:35:37.71 AIV.dial :bypass @14:35:37.72 PSV.dial :mixloop @14:35:37.73 PTV.dial :PRVmixing @14:35:37.75 PRV.dial AIV @14:35:37.76 CTV.dial :air,avoiding: :intake @14:35:37.78 Loading lyfil puck from tube 4 into Collection Clamp @14:35:38.03 SC.reconfigure SCtight @14:35:38.08 SC.seek 4 @14:35:40.20 SC.reconfigure SCloose @14:35:40.43 Elbow.reconfigure TightElbow @14:35:40.48 Elbow.seek 4 @14:35:42.50 Forearm.reconfigure TightForearm @14:35:42.55 Forearm.seek 4 + 700 counts @14:35:43.68 SE.reconfigure SEpick @14:35:43.75 SE.seek beamBreak @14:35:44.97 SE.configure SEconfig @14:35:45.14 Elbow.seek 4 @14:35:45.34 Forearm.seek 4 + 300 counts @14:35:45.55 Forearm.reconfigure LooseForearm @14:35:45.59 Hand.close @14:35:46.26 SE.seek :down @14:35:46.50
Forearm.seek :retract @14:35:47.35 SE.coast @14:35:47.45
Elbow.seek CC @14:35:49.15 Elbow.reconfigure LooseElbow @14:35:49.25 Forearm.reconfigure TightForearm @14:35:49.30 Forearm.seek CC @14:35:50.59 CC.seek :closed @14:35:54.84 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.30 @14:35:55.36 Hand.open @14:35:56.07 Forearm.reconfigure LooseForearm @14:35:56.11 Forearm.seek :retract @14:35:57.10 SC.coast @14:35:57.14 CTV.dial CSV,avoiding: :intake @14:35:58.57 CSV.dial CTV @14:35:58.90 CS.seek 5ml @14:36:12.65 delay 1:20 @14:37:32.68 CS.seek 2.5ml @14:37:40.06 delay 40 seconds @14:38:20.09 CS.seek 0ml @14:38:27.56 delay 1:00 @14:39:27.58 PSV.dial :air @14:39:28.07 PS.seek 2.5ml @14:39:54.31 PSV.dial :mixloop @14:39:54.79 delay 3 seconds @14:39:57.80 PSV.dial :air @14:39:58.29 delay 2 seconds @14:40:00.30 PSV.dial :mixloop @14:40:00.79 delay 3 seconds @14:40:03.80 PSV.dial :waste1 @14:40:05.20 PS.seek :empty @14:40:31.28 CBV.dial :waste1 @14:40:31.80 delay 5 seconds @14:40:36.82 CBV.dial AIV @14:40:37.34 delay 10 seconds @14:40:47.36 CBV.dial :waste1 @14:40:47.86 delay 10 seconds @14:40:57.88 CBV.dial AIV @14:40:58.39 delay 10 seconds @14:41:08.42 CTV.dial CSV,avoiding: :intake @14:41:08.43 CBV.dial between AIV and waste1 @14:41:08.78 CSV.dial :air @14:41:09.27 CS.seek 10ml @14:41:35.74 CSV.dial CTV @14:41:36.24 CS.seek 0ml @14:42:02.77 CBV.dial :waste1 @14:42:03.11 delay 10 seconds @14:42:13.14 CTV.dial :air,avoiding: :intake @14:42:14.61 Storing lyfil puck in Collection Clamp to tube 3 @14:42:14.80 SC.reconfigure SCtight @14:42:14.84 SC.seek 3 @14:42:16.97 SC.reconfigure SCloose @14:42:17.19 Elbow.reconfigure TightElbow @14:42:17.24 Elbow.seek 3 @14:42:19.12 Forearm.reconfigure TightForearm @14:42:19.17 Forearm.seek 3 + 700 counts @14:42:20.25 SE.reconfigure SEpick @14:42:20.32 SE.seek beamBreak @14:42:23.30 SE.configure SEconfig @14:42:23.47 Elbow.reconfigure LimpElbow @14:42:23.52 Forearm.reconfigure LooseForearm @14:42:23.58 Forearm.seek 3 @14:42:23.90 Elbow.hold @14:42:23.97 Elbow.reconfigure TightElbow @14:42:24.09 SE.seek 1.07 Pucks below Top Plate @14:42:24.54 Forearm.force -20 @14:42:24.58 Forearm.seek 3 + 329 counts @14:42:24.90 Forearm.reconfigure TightForearm @14:42:24.95 Forearm.seek 3 + 700 counts @14:42:25.27 SE.seek 2.07 Pucks below Top Plate @14:42:25.88 Forearm.reconfigure LooseForearm @14:42:25.92 Forearm.seek :retract @14:42:26.84 Elbow.seek CC @14:42:28.58 Elbow.reconfigure LooseElbow @14:42:29.21 Forearm.seek CC @14:42:30.17 Hand.close @14:42:30.88 CC.seek :open @14:42:35.38 Forearm.seek :retract @14:42:36.38 Elbow.reconfigure TightElbow @14:42:36.43 Elbow.seek 3 @14:42:38.33 Elbow.reconfigure LimpElbow @14:42:38.38 Forearm.seek 3 @14:42:39.37 Elbow.hold @14:42:39.44 Elbow.reconfigure TightElbow @14:42:39.49 Hand.open @14:42:40.40 Forearm.force -20 @14:42:40.44 Forearm.seek 3 + 350 counts @14:42:40.76 Forearm.reconfigure TightForearm @14:42:40.81 Forearm.seek 3 + 700 counts @14:42:41.15 SE.seek :down @14:42:42.30
Forearm.reconfigure LooseForearm @14:42:42.35 Forearm.seek :retract @14:42:43.79 SE.coast @14:42:43.83
SC.coast @14:42:43.87 PSV.dial :air @14:42:45.04 PS.seek 0.499ml @14:42:50.95 delay 3 seconds @14:42:53.96 PSV.dial :mixloop @14:42:54.56 PRV.dial :air @14:42:55.04 delay 3 seconds @14:42:58.06 PRV.dial CBV @14:42:58.55 CBV.dial AIV @14:42:59.08 PRV.dial between CBV and air @14:42:59.43 PRV.dial :air @14:42:59.82 PS.reconfigure PSslow @14:42:59.86 PS.seek 0.599ml @14:43:02.38 PRV.dial :waste2 @14:43:03.18 PS.reconfigure PSconfig @14:43:03.23 PS.seek :empty @14:43:10.11 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:43:10.18 Hand.open @14:43:10.51 Elbow.seek FlushPuck::Garage @14:43:12.48 Elbow.reconfigure LooseElbow @14:43:12.58 Forearm.seek :garage @14:43:13.59 Hand.close @14:43:14.39 Forearm.seek :retract @14:43:15.39 Elbow.reconfigure TightElbow @14:43:15.44 Elbow.seek CC @14:43:18.29 Elbow.reconfigure LooseElbow @14:43:18.39 Forearm.reconfigure TightForearm @14:43:18.44 Forearm.seek CC @14:43:19.72 CC.seek :closed @14:43:23.97 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.16 @14:43:24.04 Hand.open @14:43:24.70 Forearm.reconfigure LooseForearm @14:43:24.74 Forearm.seek :retract @14:43:25.81 Clearing PML for modified lysate @14:43:25.82 CTV.dial :air,avoiding: :intake @14:43:25.83 PRV.dial :waste2 @14:43:25.85 PTV.dial :PRV,avoiding: :puck @14:43:26.67 delay 2 seconds @14:43:28.69 PTV.dial :PRVmixing,avoiding: :puck @14:43:29.48 delay 2 seconds @14:43:31.49 PSV.dial :PML @14:43:31.51 delay 2 seconds @14:43:33.53 PRV.dial :air @14:43:34.34 delay 2 seconds @14:43:36.42 PS.seek 1.499ml @14:43:52.45 delay 5 seconds @14:43:57.48 PS.seek 2.5ml @14:44:08.44 PSV.dial :air @14:44:08.90 PS.seek 0ml @14:44:35.11 mixing lysate with speDil @14:44:35.13 PSV.dial :PML @14:44:35.64 CBV.dial :PRV @14:44:35.65 PRV.dial :air @14:44:35.66 delay 2 seconds @14:44:37.68 PRV.dial :speDil @14:44:38.19 delay 2 seconds @14:44:40.28 PS.reconfigure PSslow1 @14:44:40.33 PS.seek 0.225ml @14:45:03.76 delay 5 seconds @14:45:08.78 PRV.dial CBV @14:45:09.61 delay 2 seconds @14:45:11.69 PS.seek 0.475ml @14:45:37.36 delay 5 seconds @14:45:42.38 PRV.dial :air @14:45:42.89 delay 2 seconds @14:45:44.91 PS.seek 0.965ml @14:46:34.86 delay 10 seconds @14:46:44.88 PRV.dial CBV @14:46:45.37 CTV.dial :air,avoiding: :intake @14:46:45.38 PSV.dial :air @14:46:45.86 PS.reconfigure PSconfig @14:46:45.91 PS.seek 2.5ml @14:47:02.23 delay 3 seconds @14:47:05.24 PSV.dial PTV @14:47:06.03 PTV.dial :PRV,avoiding: :puck @14:47:06.83 PS.seek 0ml @14:47:33.04 PSV.dial :air @14:47:33.84 PS.seek 2.5ml @14:47:59.98 delay 3 seconds @14:48:03.00 PSV.dial PTV @14:48:03.78 PTV.dial :PRV,avoiding: :puck @14:48:03.80 PS.seek 0ml @14:48:29.89 Pushing modified lysate into AIV Injection Loop @14:48:29.90 CBV.dial AIV @14:48:29.91 AIV.dial :bypass @14:48:29.92 PRV.dial CBV @14:48:29.93 PSV.dial :air @14:48:30.74 PS.seek 2.5ml @14:48:56.95 delay 3 seconds @14:48:59.96 PSV.dial :PML @14:49:00.44 PTV.dial :PRVmixing,avoiding: :puck @14:49:01.31 PS.reconfigure PSslow1 @14:49:01.36 PS.seek 1.74ml @14:50:18.77 delay 5 seconds @14:50:23.79 AIV.dial :sample @14:50:24.41 AWV.select :waste @14:50:24.60 PS.reconfigure PSslow2 @14:50:24.64 PS.seek 1.22ml @14:52:12.89 delay 15 seconds @14:52:27.91 AIV.dial :bypass @14:52:28.53 PS.reconfigure PSconfig @14:52:29.09 PS.seek 0ml @14:52:42.26 PSV.dial :air @14:52:42.76 PS.seek 2.5ml @14:53:08.82 PSV.dial :PML @14:53:09.30 PS.seek 0ml @14:53:35.45 AIV.dial :coilToMFB @14:53:35.47 AWV.select :air @14:53:35.64 Extracting Nucleic Acid from AIV @14:53:35.66
Beginning samplePassD @14:53:35.70 SPE.hold 2:46:40,55C @14:53:35.80 ASV.dial :SPEdelivery @14:53:35.87
AWV.select :air @14:53:35.88 AIV.dial :bypass @14:53:35.90 PSV.dial PSR @14:53:36.20 delay 20 seconds @14:53:37.11
Pulling 1.5ml of PSR.killMFB @14:53:37.12 PSR.select :killMFB @14:53:37.25 PS.seek 1.5ml @14:53:53.24 delay 5 seconds @14:53:56.23 primeLysateINT @14:53:56.24 AWV.select :air @14:53:56.25 AIV.dial :coilToMFB @14:53:56.26 ATV.dial AIV @14:53:58.02 AS.reconfigure ASslow1 @14:53:58.07 AS.seek 0.299ml @14:53:58.25
PSV.dial :waste1 @14:53:58.77 PS.seek 0ml @14:54:14.89 PSV.dial PSR @14:54:15.40 PSR.select :air @14:54:15.54 PS.seek 2.5ml @14:54:41.65 PRV.dial CBV @14:54:41.67
CBV.dial PRV @14:54:41.70 PSV.dial :mixloop @14:54:42.83 CTV.dial :air,avoiding: :intake @14:54:42.84 PTV.dial :PRVmixing @14:54:42.86 PS.seek 0ml @14:54:58.70 delay 10 seconds @14:55:08.72 ATV.dial :air2 @14:55:08.98
PSV.dial PSR @14:55:09.57 AS.seek 0.329ml @14:55:10.17
Pulling 1.5ml of PSR.flush @14:55:10.18 PSR.select :flush @14:55:10.31 PS.seek 1.5ml @14:55:15.86 ATV.dial :waste2 @14:55:16.42 AS.reconfigure ASnorm @14:55:16.47 AS.seek :empty @14:55:26.30
delay 5 seconds @14:55:31.32 PSV.dial :waste1 @14:55:31.86 PS.seek 0ml @14:55:33.48 Lysate primed! @14:55:33.49 AFV.select :holdingCoil @14:55:33.50 ATV.dial :air2 @14:55:34.12 AS.reconfigure ASslow1 @14:55:34.18 AS.seek 0.069ml @14:55:47.95
PSV.dial PSR @14:55:48.53 delay 3 seconds @14:55:48.60
PSR.select :air @14:55:48.73 PS.seek 2.5ml @14:55:51.55 ATV.dial :AIV @14:55:52.40 AS.seek 0.469ml @14:56:14.83 PRV.dial CBV @14:56:14.86
CBV.dial PRV @14:56:14.87 PSV.dial :mixloop @14:56:16.02 CTV.dial :air,avoiding: :intake @14:56:16.03 PTV.dial :PRVmixing @14:56:16.05 PS.seek 0ml @14:56:42.29 PSV.dial PSR @14:56:43.49 Pulling 1.5ml of PSR.flush @14:56:43.50 PSR.select :flush @14:56:43.63 PS.seek 1.5ml @14:56:59.68 delay 5 seconds @14:57:04.70 PSV.dial :waste1 @14:57:05.21 PS.seek 0ml @14:57:13.08 delay 5 seconds @14:57:18.17 Solid Phase Extraction Heater at 47.6C @14:57:18.19 ATV.dial ASV @14:57:18.59 ASV.dial :SPEdelivery @14:57:18.67 AS.seek 0.041ml @14:57:21.32
PSV.dial PSR @14:57:21.82 PSR.select :air @14:57:21.95 PS.seek 2.5ml @14:57:48.05 PRV.dial CBV @14:57:48.08
CBV.dial PRV @14:57:48.09 PSV.dial :mixloop @14:57:49.21 CTV.dial :air,avoiding: :intake @14:57:49.22 PTV.dial :PRVmixing @14:57:49.24 PS.seek 0ml @14:58:15.45 PSV.dial PSR @14:58:16.63 Pulling 1.5ml of PSR.flush @14:58:16.64 PSR.select :flush @14:58:16.77 PS.seek 1.5ml @14:58:32.77 delay 5 seconds @14:58:37.79 PSV.dial :waste1 @14:58:38.31 PS.seek 0ml @14:58:45.56 delay 10 seconds @14:58:54.29
PSV.dial PSR @14:58:54.79 PSR.select :air @14:58:54.92 PS.seek 2.5ml @14:58:55.58 rinseLysateLineINT @14:58:55.59 AFV.select :carrierSolution @14:58:55.70 delay 2 seconds @14:58:57.73 AS.reconfigure ASconfig @14:58:57.78 AS.seek 1ml @14:59:21.02 PRV.dial CBV @14:59:21.05
CBV.dial PRV @14:59:21.06 PSV.dial :mixloop @14:59:22.29 CTV.dial :air,avoiding: :intake @14:59:22.30 PTV.dial :PRVmixing @14:59:22.32 PS.reconfigure PSslow @14:59:22.37 PS.seek 1.2ml @14:59:49.15 PSV.dial :waste1 @14:59:50.57 PS.reconfigure PSconfig @14:59:50.62 PS.seek 0ml @14:59:53.17 delay 1 second @14:59:54.18 AFV.select :holdingCoil @14:59:54.30 delay 1 second @14:59:55.32 ATV.dial AIV @14:59:55.86 AIV.dial :coilToMFB @14:59:55.87 AWV.select :waste @14:59:55.98 AS.reconfigure ASslow0 @14:59:56.03 AS.seek 0ml @15:00:03.67
PSV.dial :air @15:00:04.92 PS.seek 2.5ml @15:00:31.05 PSV.dial :mixloop @15:00:31.56 PS.reconfigure PSslow @15:00:31.61 PS.seek 0ml @15:01:22.73 PSV.dial :air @15:01:23.23 PS.reconfigure PSconfig @15:01:23.28 PS.seek 2.5ml @15:01:36.91 ATV.dial :air2 @15:01:37.67 AS.reconfigure ASslow1 @15:01:37.72 AS.seek 0.05ml @15:01:48.05 ATV.dial :AIV @15:01:48.85 AS.seek 0.01ml @15:01:49.42
PSV.dial :mixloop @15:01:49.92 PS.reconfigure PSslow @15:01:49.97 PS.seek 0ml @15:01:57.16 ATV.dial :waste @15:01:58.73 AS.seek :empty @15:02:01.16 ethanol wash @15:02:01.17 ATV.dial :air2 @15:02:02.39 AS.reconfigure ASslow2 @15:02:02.44 AS.seek 0.009ml @15:02:12.78 delay 3 seconds @15:02:15.80 Pulling 0.4ml of Ethanol Wash @15:02:15.81 ATV.dial :ethanol @15:02:16.61 AS.reconfigure ASslow1 @15:02:16.66 AS.seek 0.409ml @15:02:41.12
Cleaning Collection Syringe @15:02:41.13 CSV.dial CSR @15:02:41.67 Pulling 4ml of CSR.flush @15:02:41.68 CSR.select :flush @15:02:41.81 CS.seek 4ml @15:02:53.11 delay 5 seconds @15:02:58.13 CSR.select :air @15:02:58.27 CS.seek 10ml @15:03:14.55 CSV.dial CTV @15:03:14.57
CTV.dial CSV @15:03:16.07 CBV.dial :waste1 @15:03:17.10 CS.seek 0ml @15:03:37.38 delay 3 seconds @15:03:40.40 ATV.dial :air1 @15:03:40.80 delay 3 seconds @15:03:43.54
CSV.dial CSR @15:03:43.87 AS.seek 0.434ml @15:03:44.10
Pulling 4ml of CSR.flush @15:03:44.11 CSR.select :flush @15:03:44.24 CS.seek 4ml @15:03:49.19 delay 3 seconds @15:03:52.21 ATV.dial :ethanol @15:03:52.82 AS.seek 0.419ml @15:03:55.40
delay 5 seconds @15:03:56.06 delay 3 seconds @15:03:59.08 ATV.dial ASV @15:04:00.37 AS.seek 0.092ml @15:04:00.49
CSR.select :air @15:04:00.62 CS.seek 10ml @15:04:16.93 CSV.dial CTV @15:04:16.96
CTV.dial CSV @15:04:17.46 CBV.dial :waste1 @15:04:17.48 CS.seek 0ml @15:04:44.00 Garaging FlushPuck in Collection Clamp @15:04:44.08 Elbow.reconfigure TightElbow @15:04:44.13 Elbow.seek CC @15:04:44.38 Elbow.reconfigure LooseElbow @15:04:44.49 Forearm.seek CC @15:04:45.47 Hand.close @15:04:46.17 CC.seek :open @15:04:50.53 Forearm.seek :retract @15:04:51.54 Elbow.reconfigure TightElbow @15:04:51.59 Elbow.seek :garage @15:04:54.66 Elbow.reconfigure LooseElbow @15:04:54.77 Forearm.reconfigure TightForearm @15:04:54.82 Forearm.seek :garage @15:04:56.02 Hand.open @15:04:56.86 Forearm.reconfigure LooseForearm @15:04:56.91 Forearm.seek :retract @15:04:57.93 Whole Cell Archive sampling 1500ml @15:04:57.95 Loading wcr puck from tube 4 into Collection Clamp @15:04:58.21 SC.reconfigure SCtight @15:04:58.26 SC.seek 4 @15:05:00.40 SC.reconfigure SCloose @15:05:00.63 Elbow.reconfigure TightElbow @15:05:00.68 Elbow.seek 4 @15:05:02.73 Forearm.reconfigure TightForearm @15:05:02.79 Forearm.seek 4 + 700 counts @15:05:03.88 SE.reconfigure SEpick @15:05:03.95 SE.seek beamBreak @15:05:05.34 SE.configure SEconfig @15:05:05.53 Elbow.seek 4 @15:05:05.80 Forearm.seek 4 + 300 counts @15:05:06.01 Forearm.reconfigure LooseForearm @15:05:06.06 Hand.close @15:05:06.47 delay 3 seconds @15:05:06.81 SE.seek :down @15:05:07.58 Forearm.seek :retract @15:05:08.56 SE.coast @15:05:08.59 Elbow.seek CC @15:05:09.48 ATV.dial :waste2 @15:05:10.24 AS.reconfigure ASconfig @15:05:10.36 AS.seek :empty @15:05:10.39 Elbow.reconfigure LooseElbow @15:05:10.55 Forearm.reconfigure TightForearm @15:05:10.60 Forearm.seek CC @15:05:11.90 CC.seek :closed @15:05:16.07 ATV.dial :air2 @15:05:16.22 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.25 @15:05:16.24 Hand.open @15:05:16.47 AS.reconfigure ASslow1 @15:05:16.52 AS.seek 1ml @15:05:16.99 Forearm.reconfigure LooseForearm @15:05:17.04 Forearm.seek :retract @15:05:18.05 SC.coast @15:05:18.10 collectSample 1500ml w/bubble point of 55psi @15:05:18.11 Priming 7ml @15:05:18.12 Intake.select :close @15:05:18.13 Exhaust.select :close @15:05:18.15 SS.hold @15:05:18.37 Calibrating pressure sensors @15:05:18.39 CTV.dial :air @15:05:19.82 SSV.dial :spit,avoiding: :exhaust @15:05:19.84 SS.reconfigure SSfast @15:05:19.89 SS.seek 5ml @15:05:22.50 SSV.dial CBV @15:05:22.53 CBV.dial SSV @15:05:24.07 delay 3.8 seconds @15:05:28.01 SSV.dial :spit @15:05:29.46 SS.seek 0ml @15:05:32.15 SSV.dial CBV @15:05:33.61 CTV.dial :intake @15:05:34.41 SSV.dial CBV @15:05:34.44 CBV.dial SSV @15:05:34.46 Allowing 40s for 7.0ml of remaining 7.0ml @15:05:34.47 Intake.select :open @15:05:36.65 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:05:36.67 SS.reconfigure SSinhale.with maxSpeed: 150 @15:05:36.72 SS.seek 7ml @15:05:40.39 Vacuum too high -- waiting up to 40s @15:05:43.02 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:05:43.04 SS.reconfigure SSinhale.with maxSpeed: 135 @15:05:43.08 SS.seek 7ml @15:05:46.96 Stroke completed -- waiting for pressure to equalize ... @15:05:51.38 Intake.select :close @15:05:51.54 SSV.dial :exhaust @15:05:52.37 Exhaust.select :open @15:05:52.48 SS.reconfigure SSexhale @15:05:52.53 SS.seek 0ml @15:05:56.30 Exhaust.select :close @15:05:56.42 Sampling 1500.0ml @15:05:56.43 Intake.select :close @15:05:56.45 Exhaust.select :close @15:05:56.46 SS.reconfigure SSconfig @15:05:56.51 SS.hold @15:05:56.80 CTV.dial :intake @15:05:56.83 SSV.dial CBV @15:05:56.86 CBV.dial SSV @15:05:57.73 Allowing 130s for 25.0ml of remaining 1500.0ml @15:05:57.74 Intake.select :open @15:05:59.92 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:05:59.94 SS.reconfigure SSinhale.with maxSpeed: 135 @15:05:59.99 SS.seek 25ml @15:06:03.67 Vacuum too high -- waiting up to 40s @15:06:05.54 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:06:05.56 SS.reconfigure SSinhale.with maxSpeed: 122 @15:06:05.61 SS.seek 25ml @15:06:08.57 Vacuum too high -- waiting up to 40s @15:06:10.43 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:06:10.45 SS.reconfigure SSinhale.with maxSpeed: 110 @15:06:10.50 SS.seek 25ml @15:06:13.23 Vacuum too high -- waiting up to 40s @15:06:14.84 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:06:14.86 SS.reconfigure SSinhale.with maxSpeed: 99 @15:06:14.91 SS.seek 25ml @15:06:17.54 Vacuum too high -- waiting up to 40s @15:06:18.90 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:06:18.92 SS.reconfigure SSinhale.with maxSpeed: 89 @15:06:18.97 SS.seek 25ml @15:06:21.51 Vacuum too high -- waiting up to 40s @15:06:23.42 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:06:23.44 SS.reconfigure SSinhale.with maxSpeed: 80 @15:06:23.50 SS.seek 25ml @15:06:26.71 Vacuum too high -- waiting up to 40s @15:06:28.07 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:06:28.09 SS.reconfigure SSinhale.with maxSpeed: 72 @15:06:28.14 SS.seek 25ml @15:06:31.51 Vacuum too high -- waiting up to 40s @15:06:32.89 SS.reconfigure SSinhale.with maxSpeed: 65 @15:06:32.94 SS.seek 25ml @15:06:36.45 Vacuum too high -- waiting up to 40s @15:06:37.82 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:06:37.84 SS.reconfigure SSinhale.with maxSpeed: 59 @15:06:37.89 SS.seek 25ml @15:06:41.60 Stroke completed -- waiting for pressure to equalize ... @15:06:44.82 Intake.select :close @15:06:44.99 SSV.dial :exhaust @15:06:45.83 Exhaust.select :open @15:06:45.94 SS.reconfigure SSexhale @15:06:45.99 SS.seek 0ml @15:06:55.18 Exhaust.select :close @15:06:55.30 SSV.dial CBV @15:06:55.33 CBV.dial SSV @15:06:56.19 Allowing 130s for 25.0ml of remaining 1475.0ml @15:06:56.20 Intake.select :open @15:06:58.38 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:06:58.40 SS.reconfigure SSinhale.with maxSpeed: 59 @15:06:58.45 SS.seek 25ml @15:07:04.85 Vacuum too high -- waiting up to 40s @15:07:05.95 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:07:05.97 SS.reconfigure SSinhale.with maxSpeed: 53 @15:07:06.02 SS.seek 25ml @15:07:14.14 Vacuum too high -- waiting up to 40s @15:07:15.22 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:07:15.24 SS.reconfigure SSinhale.with maxSpeed: 48 @15:07:15.29 SS.seek 25ml @15:07:34.10 Vacuum too high -- waiting up to 40s @15:07:35.20 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:07:35.78 SS.reconfigure SSinhale.with maxSpeed: 43 @15:07:35.83 SS.seek 25ml @15:07:39.36 Stroke completed -- waiting for pressure to equalize ... @15:07:41.96 Intake.select :close @15:07:42.13 SSV.dial :exhaust @15:07:42.95 Exhaust.select :open @15:07:43.06 SS.reconfigure SSexhale @15:07:43.11 SS.seek 0ml @15:07:52.30 Exhaust.select :close @15:07:52.42 SSV.dial CBV @15:07:52.45 CBV.dial SSV @15:07:53.32 Allowing 130s for 25.0ml of remaining 1450.0ml @15:07:53.35 Intake.select :open @15:07:55.56 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:07:55.58 SS.reconfigure SSinhale.with maxSpeed: 43 @15:07:55.64 SS.seek 25ml @15:08:36.96 Stroke completed -- waiting for pressure to equalize ... @15:08:38.07 delay 5 seconds @15:08:40.21 Intake.select :close @15:08:40.37 SSV.dial :exhaust @15:08:41.20 Exhaust.select :open @15:08:41.31 SS.reconfigure SSexhale @15:08:41.36 SS.seek 0ml @15:08:43.09 ATV.dial ASV @15:08:44.04 ASV.dial :SPEdelivery @15:08:44.07 AS.seek 0.02ml @15:08:50.46 Exhaust.select :close @15:08:50.58 SSV.dial CBV @15:08:50.61 CBV.dial SSV @15:08:51.47 Allowing 130s for 25.0ml of remaining 1425.0ml @15:08:51.48 Intake.select :open @15:08:53.66 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:08:53.68 SS.reconfigure SSinhale.with maxSpeed: 43 @15:08:53.73 SS.seek 25ml @15:09:34.94 Stroke completed -- waiting for pressure to equalize ... @15:09:38.17 Intake.select :close @15:09:38.34 SSV.dial :exhaust @15:09:39.19 Exhaust.select :open @15:09:39.30 SS.reconfigure SSexhale @15:09:39.35 SS.seek 0ml @15:09:48.43 Exhaust.select :close @15:09:48.55 SSV.dial CBV @15:09:48.58 CBV.dial SSV @15:09:49.44 Allowing 130s for 25.0ml of remaining 1400.0ml @15:09:49.46 Intake.select :open @15:09:51.63 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:09:51.65 SS.reconfigure SSinhale.with maxSpeed: 43 @15:09:51.70 SS.seek 25ml @15:10:30.92 Vacuum too high -- waiting up to 40s @15:10:32.01 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:10:32.03 SS.reconfigure SSinhale.with maxSpeed: 39 @15:10:32.08 SS.seek 25ml @15:10:34.79 Stroke completed -- waiting for pressure to equalize ... @15:10:37.94 Intake.select :close @15:10:38.10 SSV.dial :exhaust @15:10:38.94 Exhaust.select :open @15:10:39.05 SS.reconfigure SSexhale @15:10:39.10 SS.seek 0ml @15:10:48.04 Exhaust.select :close @15:10:48.17 SSV.dial CBV @15:10:48.23 CBV.dial SSV @15:10:49.11 Allowing 130s for 25.0ml of remaining 1375.0ml @15:10:49.12 Intake.select :open @15:10:51.31 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:10:51.33 SS.reconfigure SSinhale.with maxSpeed: 39 @15:10:51.38 SS.seek 25ml @15:11:36.97 Stroke completed -- waiting for pressure to equalize ... @15:11:40.19 Intake.select :close @15:11:40.36 SSV.dial :exhaust @15:11:41.20 Exhaust.select :open @15:11:41.31 SS.reconfigure SSexhale @15:11:41.36 SS.seek 0ml @15:11:50.19 Exhaust.select :close @15:11:50.31 SSV.dial CBV @15:11:50.34 CBV.dial SSV @15:11:51.20 Allowing 130s for 25.0ml of remaining 1350.0ml @15:11:51.21 Intake.select :open @15:11:53.40 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:11:53.42 SS.reconfigure SSinhale.with maxSpeed: 39 @15:11:53.47 SS.seek 25ml @15:12:01.43 delay 5 seconds @15:12:06.45 ATV.dial :air2 @15:12:07.50 AS.seek 0.52ml @15:12:39.26 Stroke completed -- waiting for pressure to equalize ... @15:12:43.13 Intake.select :close @15:12:43.29 SSV.dial :exhaust @15:12:44.12 Exhaust.select :open @15:12:44.23 SS.reconfigure SSexhale @15:12:44.29 SS.seek 0ml @15:12:53.46 Exhaust.select :close @15:12:53.58 SSV.dial CBV @15:12:53.61 CBV.dial SSV @15:12:54.47 Allowing 130s for 25.0ml of remaining 1325.0ml @15:12:54.48 Intake.select :open @15:12:56.66 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:12:56.68 SS.reconfigure SSinhale.with maxSpeed: 39 @15:12:56.73 SS.seek 25ml @15:13:42.02 Stroke completed -- waiting for pressure to equalize ... @15:13:46.49 Intake.select :close @15:13:46.65 SSV.dial :exhaust @15:13:47.51 Exhaust.select :open @15:13:47.62 SS.reconfigure SSexhale @15:13:47.67 SS.seek 0ml @15:13:48.41 delay 5 seconds @15:13:53.43 ATV.dial ASV @15:13:54.39 ASV.dial :wasteR @15:13:54.76 AS.seek 0.3ml @15:13:57.19 Exhaust.select :close @15:13:57.31 SSV.dial CBV @15:13:57.34 CBV.dial SSV @15:13:58.74 Allowing 130s for 25.0ml of remaining 1300.0ml @15:13:58.75 Intake.select :open @15:14:00.92 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:14:00.95 SS.reconfigure SSinhale.with maxSpeed: 39 @15:14:01.00 SS.seek 25ml @15:14:33.89 Vacuum too high -- waiting up to 40s @15:14:35.01 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:14:35.03 SS.reconfigure SSinhale.with maxSpeed: 35 @15:14:35.08 SS.seek 25ml @15:14:39.29 delay 20 seconds @15:14:49.30 Stroke completed -- waiting for pressure to equalize ... @15:14:53.19 Intake.select :close @15:14:53.35 SSV.dial :exhaust @15:14:54.17 Exhaust.select :open @15:14:54.28 SS.reconfigure SSexhale @15:14:54.34 SS.seek 0ml @15:14:59.32 AS.seek 0.07ml @15:15:03.52 Exhaust.select :close @15:15:03.64 SSV.dial CBV @15:15:03.67 CBV.dial SSV @15:15:04.52 Allowing 130s for 25.0ml of remaining 1275.0ml @15:15:04.54 Intake.select :open @15:15:06.71 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:15:06.73 SS.reconfigure SSinhale.with maxSpeed: 35 @15:15:06.78 SS.seek 25ml @15:15:45.86 delay 20 seconds @15:15:56.80 Stroke completed -- waiting for pressure to equalize ... @15:16:00.66 Intake.select :close @15:16:00.82 SSV.dial :exhaust @15:16:01.63 Exhaust.select :open @15:16:01.75 SS.reconfigure SSexhale @15:16:01.80 SS.seek 0ml @15:16:05.87 ATV.dial :waste2 @15:16:06.61 AS.reconfigure ASconfig @15:16:06.66 AS.seek :empty @15:16:11.06 Exhaust.select :close @15:16:11.08 Elution @15:16:11.14 ATV.dial :air1 @15:16:11.20 SSV.dial CBV @15:16:11.25 CBV.dial SSV @15:16:12.06 Allowing 130s for 25.0ml of remaining 1250.0ml @15:16:12.07 Intake.select :open @15:16:12.24 AS.reconfigure ASslow1 @15:16:12.30 AS.seek 0.049ml @15:16:14.38 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:16:14.40 SS.reconfigure SSinhale.with maxSpeed: 35 @15:16:14.44 SS.seek 25ml @15:16:22.65 delay 3 seconds @15:16:25.67 ATV.dial :ElutionWater @15:16:26.34 AS.seek 0.109ml @15:16:38.66 delay 3 seconds @15:16:41.69 ATV.dial ASV,via: :air2 @15:16:43.72 ASV.dial :SPEelution @15:16:44.28 AFV.select :carrierSolution @15:16:44.45 AS.reconfigure ASnorm @15:16:44.50 AS.seek 0.759ml @15:17:04.68 Stroke completed -- waiting for pressure to equalize ... @15:17:09.08 Intake.select :close @15:17:09.24 SSV.dial :exhaust @15:17:10.06 Exhaust.select :open @15:17:10.17 SS.reconfigure SSexhale @15:17:10.22 SS.seek 0ml @15:17:17.55 delay 3 seconds @15:17:19.51 Exhaust.select :close @15:17:19.63 SSV.dial CBV @15:17:19.66 CBV.dial SSV @15:17:20.52 Allowing 130s for 25.0ml of remaining 1225.0ml @15:17:20.53 Intake.select :open @15:17:20.59 AFV.select :holdingCoil @15:17:20.75 AS.reconfigure ASslow1 @15:17:20.80 AS.seek 0.474ml @15:17:22.72 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:17:22.74 SS.reconfigure SSinhale.with maxSpeed: 35 @15:17:22.79 SS.seek 25ml @15:18:12.91 Stroke completed -- waiting for pressure to equalize ... @15:18:18.05 Intake.select :close @15:18:18.22 SSV.dial :exhaust @15:18:18.41 delay 5 seconds @15:18:19.06 Exhaust.select :open @15:18:19.17 SS.reconfigure SSexhale @15:18:19.22 SS.seek 0ml @15:18:23.49 AS.reconfigure ASslow2 @15:18:23.54 AS.seek 0.439ml @15:18:28.15 Exhaust.select :close @15:18:28.27 SSV.dial CBV @15:18:28.30 CBV.dial SSV @15:18:29.17 Allowing 130s for 25.0ml of remaining 1200.0ml @15:18:29.18 Intake.select :open @15:18:31.36 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:18:31.38 SS.reconfigure SSinhale.with maxSpeed: 35 @15:18:31.43 SS.seek 25ml @15:18:58.88 delay 1:00 @15:19:09.23 Vacuum too high -- waiting up to 40s @15:19:10.59 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:19:10.61 SS.reconfigure SSinhale.with maxSpeed: 32 @15:19:10.65 SS.seek 25ml @15:19:24.86 Stroke completed -- waiting for pressure to equalize ... @15:19:29.34 Intake.select :close @15:19:29.51 SSV.dial :exhaust @15:19:30.33 Exhaust.select :open @15:19:30.45 SS.reconfigure SSexhale @15:19:30.50 SS.seek 0ml @15:19:39.72 Exhaust.select :close @15:19:39.84 SSV.dial CBV @15:19:39.87 CBV.dial SSV @15:19:40.73 Allowing 130s for 25.0ml of remaining 1175.0ml @15:19:40.74 Intake.select :open @15:19:42.92 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:19:42.94 SS.reconfigure SSinhale.with maxSpeed: 32 @15:19:42.99 SS.seek 25ml @15:19:58.91 pushing eluate to holding coil @15:19:59.53 AS.seek 0.279ml @15:20:38.05 Stroke completed -- waiting for pressure to equalize ... @15:20:43.21 Intake.select :close @15:20:43.37 SSV.dial :exhaust @15:20:44.20 Exhaust.select :open @15:20:44.31 SS.reconfigure SSexhale @15:20:44.36 SS.seek 0ml @15:20:53.60 Exhaust.select :close @15:20:53.72 SSV.dial CBV @15:20:53.75 CBV.dial SSV @15:20:54.61 Allowing 130s for 25.0ml of remaining 1150.0ml @15:20:54.62 Intake.select :open @15:20:56.80 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:20:56.83 SS.reconfigure SSinhale.with maxSpeed: 32 @15:20:56.88 SS.seek 25ml @15:21:51.79 Stroke completed -- waiting for pressure to equalize ... @15:21:57.55 Intake.select :close @15:21:57.71 SSV.dial :exhaust @15:21:58.52 Exhaust.select :open @15:21:58.64 SS.reconfigure SSexhale @15:21:58.69 SS.seek 0ml @15:22:07.80 Exhaust.select :close @15:22:07.92 SSV.dial CBV @15:22:07.95 CBV.dial SSV @15:22:08.80 Allowing 130s for 25.0ml of remaining 1125.0ml @15:22:08.81 Intake.select :open @15:22:10.99 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:22:11.01 SS.reconfigure SSinhale.with maxSpeed: 32 @15:22:11.06 SS.seek 25ml @15:22:40.55 delay 30 seconds @15:22:49.64 Vacuum too high -- waiting up to 40s @15:22:50.99 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:22:51.01 SS.reconfigure SSinhale.with maxSpeed: 29 @15:22:51.06 SS.seek 25ml @15:23:09.72 Stroke completed -- waiting for pressure to equalize ... @15:23:10.57 ATV.dial :waste,via: :air3 @15:23:12.26 AS.reconfigure ASconfig @15:23:12.31 AS.seek :empty @15:23:14.84 Intake.select :close @15:23:15.00 SSV.dial :exhaust @15:23:15.84 Exhaust.select :open @15:23:16.42 SS.reconfigure SSexhale @15:23:16.56 SS.seek 0ml @15:23:25.79 Exhaust.select :close @15:23:25.91 SSV.dial CBV @15:23:25.94 CBV.dial SSV @15:23:26.81 Allowing 130s for 25.0ml of remaining 1100.0ml @15:23:26.82 Intake.select :open @15:23:28.71 Parking Bleach in LysateSource line and SPEcolumn @15:23:28.72 ATV.dial :air3 @15:23:29.01 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:23:29.03 SS.reconfigure SSinhale.with maxSpeed: 29 @15:23:29.08 SS.seek 25ml @15:23:29.53 AS.reconfigure ASslow1 @15:23:29.58 AS.seek 0.029ml @15:23:35.92 ATV.dial :ElutionWater @15:23:36.55 AS.seek 0.059ml @15:23:42.84 ATV.dial :air3 @15:23:43.47 AS.seek 0.069ml @15:23:45.76 delay 2 seconds @15:23:47.77 ATV.dial :bleach @15:23:48.23 AS.seek 0.769ml @15:24:29.55 Stroke completed -- waiting for pressure to equalize ... @15:24:35.33 Intake.select :close @15:24:35.49 SSV.dial :exhaust @15:24:36.33 Exhaust.select :open @15:24:36.44 SS.reconfigure SSexhale @15:24:36.49 SS.seek 0ml @15:24:45.76 Exhaust.select :close @15:24:45.88 SSV.dial CBV @15:24:45.91 CBV.dial SSV @15:24:46.77 Allowing 130s for 25.0ml of remaining 1075.0ml @15:24:46.78 Intake.select :open @15:24:48.96 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:24:48.98 SS.reconfigure SSinhale.with maxSpeed: 29 @15:24:49.03 SS.seek 25ml @15:25:49.40 Stroke completed -- waiting for pressure to equalize ... @15:25:55.78 Intake.select :close @15:25:55.95 SSV.dial :exhaust @15:25:56.77 Exhaust.select :open @15:25:56.89 SS.reconfigure SSexhale @15:25:56.94 SS.seek 0ml @15:26:06.16 Exhaust.select :close @15:26:06.28 SSV.dial CBV @15:26:06.31 CBV.dial SSV @15:26:07.16 Allowing 130s for 25.0ml of remaining 1050.0ml @15:26:07.17 Intake.select :open @15:26:09.26 delay 3 seconds @15:26:09.35 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:26:09.37 SS.reconfigure SSinhale.with maxSpeed: 29 @15:26:09.42 SS.seek 25ml @15:26:12.28 ATV.dial :air3 @15:26:12.84 delay 3 seconds @15:26:15.92 AS.seek 0.794ml @15:26:21.23 delay 3 seconds @15:26:24.25 ATV.dial :bleach @15:26:25.41 AS.seek 0.779ml @15:26:28.68 delay 3 seconds @15:26:31.70 ATV.dial :AIV @15:26:32.93 AS.reconfigure ASconfig @15:26:32.98 AS.seek 0.015ml @15:26:54.29 Vacuum too high -- waiting up to 40s @15:26:55.91 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:26:55.94 SS.reconfigure SSinhale.with maxSpeed: 26 @15:26:55.99 SS.seek 25ml @15:27:13.69 Stroke completed -- waiting for pressure to equalize ... @15:27:17.17 ATV.dial :waste @15:27:18.62 AS.seek :empty @15:27:19.49 Intake.select :close @15:27:19.65 SSV.dial :exhaust @15:27:19.82 ATV.dial :air3 @15:27:20.50 Exhaust.select :open @15:27:20.52 AS.reconfigure ASslow1 @15:27:20.61 AS.seek 0.009ml @15:27:20.67 SS.reconfigure SSexhale @15:27:20.72 SS.seek 0ml @15:27:22.95 delay 2 seconds @15:27:24.96 ATV.dial :bleach @15:27:25.42 AS.seek 0.579ml @15:27:29.86 Exhaust.select :close @15:27:29.98 SSV.dial CBV @15:27:30.05 CBV.dial SSV @15:27:30.86 Allowing 130s for 25.0ml of remaining 1025.0ml @15:27:30.87 Intake.select :open @15:27:33.05 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:27:33.07 SS.reconfigure SSinhale.with maxSpeed: 26 @15:27:33.12 SS.seek 25ml @15:28:40.57 Stroke completed -- waiting for pressure to equalize ... @15:28:46.95 Intake.select :close @15:28:47.11 SSV.dial :exhaust @15:28:47.93 Exhaust.select :open @15:28:48.05 SS.reconfigure SSexhale @15:28:48.10 SS.seek 0ml @15:28:57.49 Exhaust.select :close @15:28:57.61 SSV.dial CBV @15:28:57.64 CBV.dial SSV @15:28:58.48 Allowing 130s for 25.0ml of remaining 1000.0ml @15:28:58.49 Intake.select :open @15:29:00.67 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:29:00.69 SS.reconfigure SSinhale.with maxSpeed: 26 @15:29:00.74 SS.seek 25ml @15:29:20.31 delay 3 seconds @15:29:23.33 ATV.dial :air3 @15:29:23.88 delay 3 seconds @15:29:27.53 AS.seek 0.604ml @15:29:32.83 delay 3 seconds @15:29:35.86 ATV.dial :bleach @15:29:36.47 AS.seek 0.589ml @15:29:39.77 delay 3 seconds @15:29:42.79 ATV.dial :ASV @15:29:43.81 ASV.dial :wasteR @15:29:44.39 AS.seek 0ml @15:30:08.30 Stroke completed -- waiting for pressure to equalize ... @15:30:15.36 Intake.select :close @15:30:15.52 SSV.dial :exhaust @15:30:16.36 Exhaust.select :open @15:30:16.47 SS.reconfigure SSexhale @15:30:16.52 SS.seek 0ml @15:30:25.79 Exhaust.select :close @15:30:25.91 SSV.dial CBV @15:30:25.94 CBV.dial SSV @15:30:26.81 Allowing 130s for 25.0ml of remaining 975.0ml @15:30:26.82 Intake.select :open @15:30:29.00 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:30:29.02 SS.reconfigure SSinhale.with maxSpeed: 26 @15:30:29.07 SS.seek 25ml @15:31:14.01 Vacuum too high -- waiting up to 40s @15:31:15.85 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:31:15.87 SS.reconfigure SSinhale.with maxSpeed: 23 @15:31:15.92 SS.seek 25ml @15:31:42.26 Stroke completed -- waiting for pressure to equalize ... @15:31:43.16 delay 1:00 @15:31:48.02 Intake.select :close @15:31:48.18 SSV.dial :exhaust @15:31:49.03 Exhaust.select :open @15:31:49.14 SS.reconfigure SSexhale @15:31:49.19 SS.seek 0ml @15:31:58.21 Exhaust.select :close @15:31:58.33 SSV.dial CBV @15:31:58.38 CBV.dial SSV @15:31:59.22 Allowing 130s for 25.0ml of remaining 950.0ml @15:31:59.23 Intake.select :open @15:32:01.42 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:32:01.44 SS.reconfigure SSinhale.with maxSpeed: 23 @15:32:01.48 SS.seek 25ml @15:32:43.19 AFV.select :carrierSolution @15:32:43.30 delay 2 seconds @15:32:45.33 AS.reconfigure ASconfig @15:32:45.37 AS.seek 1ml @15:33:18.29 Stroke completed -- waiting for pressure to equalize ... @15:33:25.28 Intake.select :close @15:33:25.44 SSV.dial :exhaust @15:33:26.28 Exhaust.select :open @15:33:26.39 SS.reconfigure SSexhale @15:33:26.44 SS.seek 0ml @15:33:35.77 Exhaust.select :close @15:33:35.89 SSV.dial CBV @15:33:35.92 CBV.dial SSV @15:33:36.78 Allowing 130s for 25.0ml of remaining 925.0ml @15:33:36.79 Intake.select :open @15:33:38.98 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:33:39.00 SS.reconfigure SSinhale.with maxSpeed: 23 @15:33:39.04 SS.seek 25ml @15:33:43.15 delay 1 second @15:33:44.16 AFV.select :holdingCoil @15:33:44.27 delay 1 second @15:33:45.29 ATV.dial ASV @15:33:45.31 ASV.dial :wasteR @15:33:45.33 AS.reconfigure ASslow1 @15:33:45.37 AS.seek 0ml @15:34:55.08 Stroke completed -- waiting for pressure to equalize ... @15:35:02.75 Intake.select :close @15:35:02.92 SSV.dial :exhaust @15:35:03.74 Exhaust.select :open @15:35:03.86 SS.reconfigure SSexhale @15:35:03.91 SS.seek 0ml @15:35:13.21 Exhaust.select :close @15:35:13.33 SSV.dial CBV @15:35:13.36 CBV.dial SSV @15:35:14.20 Allowing 130s for 25.0ml of remaining 900.0ml @15:35:14.21 Intake.select :open @15:35:16.40 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:35:16.42 SS.reconfigure SSinhale.with maxSpeed: 23 @15:35:16.47 SS.seek 25ml @15:36:15.10 Vacuum too high -- waiting up to 40s @15:36:17.21 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:36:17.23 SS.reconfigure SSinhale.with maxSpeed: 21 @15:36:17.28 SS.seek 25ml @15:36:37.01 Stroke completed -- waiting for pressure to equalize ... @15:36:44.58 Intake.select :close @15:36:44.75 SSV.dial :exhaust @15:36:45.59 Exhaust.select :open @15:36:45.70 SS.reconfigure SSexhale @15:36:45.75 SS.seek 0ml @15:36:54.91 Exhaust.select :close @15:36:55.03 SSV.dial CBV @15:36:55.06 CBV.dial SSV @15:36:55.92 Allowing 130s for 25.0ml of remaining 875.0ml @15:36:55.93 Intake.select :open @15:36:58.12 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:36:58.14 SS.reconfigure SSinhale.with maxSpeed: 21 @15:36:58.18 SS.seek 25ml @15:37:06.78 delay 20 seconds @15:37:26.80 ATV.dial :air3 @15:37:28.28 AS.seek 0.59ml @15:38:21.82 Stroke completed -- waiting for pressure to equalize ... @15:38:30.12 Intake.select :close @15:38:30.28 SSV.dial :exhaust @15:38:31.11 Exhaust.select :open @15:38:31.23 SS.reconfigure SSexhale @15:38:31.28 SS.seek 0ml @15:38:40.61 Exhaust.select :close @15:38:40.73 SSV.dial CBV @15:38:40.76 CBV.dial SSV @15:38:41.61 Allowing 130s for 25.0ml of remaining 850.0ml @15:38:41.62 Intake.select :open @15:38:43.79 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:38:43.82 SS.reconfigure SSinhale.with maxSpeed: 21 @15:38:43.86 SS.seek 25ml @15:39:27.25 delay 3 seconds @15:39:30.27 ATV.dial :ASV @15:39:31.64 ASV.dial :wasteR @15:39:31.73 AS.seek 0.09ml @15:39:53.07 Vacuum too high -- waiting up to 40s @15:39:55.16 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:39:55.18 SS.reconfigure SSinhale.with maxSpeed: 19 @15:39:55.22 SS.seek 25ml @15:40:10.96 Stroke completed -- waiting for pressure to equalize ... @15:40:19.19 Intake.select :close @15:40:19.35 SSV.dial :exhaust @15:40:20.17 Exhaust.select :open @15:40:20.28 SS.reconfigure SSexhale @15:40:20.33 SS.seek 0ml @15:40:29.80 Exhaust.select :close @15:40:29.92 SSV.dial CBV @15:40:29.95 CBV.dial SSV @15:40:30.80 Allowing 130s for 25.0ml of remaining 825.0ml @15:40:30.81 Intake.select :open @15:40:33.00 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:40:33.02 SS.reconfigure SSinhale.with maxSpeed: 19 @15:40:33.06 SS.seek 25ml @15:41:12.56 delay 30 seconds @15:41:42.58 ATV.dial :extSrc @15:41:43.34 delay 3 seconds @15:41:46.36 ATV.dial :ElutionWater @15:41:47.46 AS.seek 0.12ml @15:41:53.81 ATV.dial :air3 @15:41:54.43 AS.seek 0.13ml @15:41:56.75 delay 2 seconds @15:41:58.76 ATV.dial :bleach @15:41:59.24 AS.seek 0.23ml @15:42:05.35 Stroke completed -- waiting for pressure to equalize ... @15:42:14.90 Intake.select :close @15:42:15.06 SSV.dial :exhaust @15:42:15.90 Exhaust.select :open @15:42:16.01 SS.reconfigure SSexhale @15:42:16.06 SS.seek 0ml @15:42:19.62 delay 3 seconds @15:42:22.64 ATV.dial :air3 @15:42:23.18 delay 3 seconds @15:42:25.21 Exhaust.select :close @15:42:25.33 SSV.dial CBV @15:42:25.36 CBV.dial SSV @15:42:26.20 Allowing 130s for 25.0ml of remaining 800.0ml @15:42:26.21 Intake.select :open @15:42:26.33 AS.seek 0.255ml @15:42:28.46 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:42:28.48 SS.reconfigure SSinhale.with maxSpeed: 19 @15:42:28.52 SS.seek 25ml @15:42:31.64 delay 3 seconds @15:42:34.65 ATV.dial :bleach @15:42:35.27 AS.seek 0.24ml @15:42:38.59 delay 3 seconds @15:42:41.60 ATV.dial :ASV @15:42:42.61 ASV.dial :SPEdelivery @15:42:42.99 AFV.select :carrierSolution @15:42:43.11 delay 2 seconds @15:42:45.14 AS.reconfigure ASconfig @15:42:45.74 AS.seek 0.5ml @15:43:01.00 delay 1 second @15:43:02.01 AFV.select :holdingCoil @15:43:02.17 AS.reconfigure ASslow1 @15:43:02.22 AS.seek 0.17ml @15:44:00.72 Stroke completed -- waiting for pressure to equalize ... @15:44:08.87 SPE.hold 2:46:40,95C @15:44:08.91 delay 3:00 @15:44:11.08 Intake.select :close @15:44:11.25 SSV.dial :exhaust @15:44:12.12 Exhaust.select :open @15:44:12.23 SS.reconfigure SSexhale @15:44:12.28 SS.seek 0ml @15:44:21.72 Exhaust.select :close @15:44:21.84 SSV.dial CBV @15:44:21.87 CBV.dial SSV @15:44:22.73 Allowing 130s for 25.0ml of remaining 775.0ml @15:44:22.74 Intake.select :open @15:44:24.92 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:44:24.94 SS.reconfigure SSinhale.with maxSpeed: 19 @15:44:24.99 SS.seek 25ml @15:45:11.54 Vacuum too high -- waiting up to 40s @15:45:13.47 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:45:13.49 SS.reconfigure SSinhale.with maxSpeed: 17 @15:45:13.54 SS.seek 25ml @15:46:04.69 Stroke completed -- waiting for pressure to equalize ... @15:46:13.76 Intake.select :close @15:46:13.92 SSV.dial :exhaust @15:46:14.81 Exhaust.select :open @15:46:14.92 SS.reconfigure SSexhale @15:46:14.97 SS.seek 0ml @15:46:24.57 Exhaust.select :close @15:46:24.69 SSV.dial CBV @15:46:25.19 CBV.dial SSV @15:46:26.17 Allowing 130s for 25.0ml of remaining 750.0ml @15:46:26.18 Intake.select :open @15:46:28.36 Intake @34.8psia, vacuum from 20.0 to 24.0psi @15:46:28.38 SS.reconfigure SSinhale.with maxSpeed: 17 @15:46:28.43 SS.seek 25ml @15:47:08.93 AFV.select :carrierSolution @15:47:09.04 delay 2 seconds @15:47:11.07 AS.reconfigure ASconfig @15:47:11.12 AS.seek 1ml @15:47:59.12 delay 1 second @15:48:00.13 AFV.select :holdingCoil @15:48:00.24 delay 1 second @15:48:01.26 ASV.dial :wasteD @15:48:02.30 AS.seek 0ml @15:48:11.57 Stroke completed -- waiting for pressure to equalize ... @15:48:21.81 Intake.select :close @15:48:21.97 SSV.dial :exhaust @15:48:22.83 Exhaust.select :open @15:48:22.94 SS.reconfigure SSexhale @15:48:22.99 SS.seek 0ml @15:48:32.19 Exhaust.select :close @15:48:32.31 SSV.dial CBV @15:48:32.34 CBV.dial SSV @15:48:33.22 Allowing 130s for 25.0ml of remaining 725.0ml @15:48:33.23 Intake.select :open @15:48:35.42 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:48:35.44 SS.reconfigure SSinhale.with maxSpeed: 17 @15:48:35.49 SS.seek 25ml @15:49:00.11 Priming Eluate @15:49:00.12 AFV.select :carrierSolution @15:49:00.23 delay 2 seconds @15:49:02.26 AS.seek 0.5ml @15:49:31.34 delay 1 second @15:49:32.35 AFV.select :holdingCoil @15:49:32.46 ATV.dial ASV @15:49:32.47 ASV.dial :SPErecovery @15:49:33.05 Swish #1 of 2 @15:49:33.14 AS.reconfigure ASslow0 @15:49:33.19 AS.seek 0.6ml @15:49:40.73 Vacuum too high -- waiting up to 40s @15:49:42.84 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:49:42.86 SS.reconfigure SSinhale.with maxSpeed: 15 @15:49:42.91 SS.seek 25ml @15:49:43.65 AS.seek 0.5ml @15:49:54.04 Swish #2 of 2 @15:49:54.13 AS.seek 0.6ml @15:50:04.60 AS.seek 0.5ml @15:50:14.98 delay 3 seconds @15:50:18.06 AS.reconfigure ASslow1 @15:50:18.11 AS.seek 0.684ml @15:50:26.08 Stroke completed -- waiting for pressure to equalize ... @15:50:36.83 Intake.select :close @15:50:37.00 SSV.dial :exhaust @15:50:37.86 Exhaust.select :open @15:50:37.97 SS.reconfigure SSexhale @15:50:38.02 SS.seek 0ml @15:50:47.77 Exhaust.select :close @15:50:47.89 SSV.dial CBV @15:50:47.92 CBV.dial SSV @15:50:48.80 Allowing 130s for 25.0ml of remaining 700.0ml @15:50:48.81 Intake.select :open @15:50:51.00 Intake @34.8psia, vacuum from 20.0 to 24.0psi @15:50:51.02 SS.reconfigure SSinhale.with maxSpeed: 15 @15:50:51.07 SS.seek 25ml @15:50:55.41 delay 3 seconds @15:50:58.44 Eluate primed @15:50:58.45 ATV.dial :waste2 @15:50:59.16 AS.reconfigure ASnorm @15:50:59.21 AS.seek 0ml @15:51:34.02 SPE.coast @15:51:34.11 ASV.dial :SPErecovery @15:51:34.13 Setting up PCR for Ss -- writing /var/log/esp/Ss18oct1915h300ml.pcr @15:51:34.15 Building PCR Slug from ASV with MM1 mix, PP2 primer @15:51:34.16 Priming with carrier @15:51:34.17 ATV.dial ARV @15:51:35.22 ARV.dial :carrier @15:51:35.57 AS.seek 0.5ml @15:52:01.16 AFV.select :carrierSolution @15:52:01.28 delay 2 seconds @15:52:03.31 AS.reconfigure ASconfig @15:52:03.36 AS.seek 1ml @15:52:32.37 delay 1 second @15:52:33.38 AFV.select :holdingCoil @15:52:33.49 delay 1 second @15:52:34.51 ATV.dial :waste @15:52:35.98 AS.reconfigure ASnorm @15:52:36.03 AS.seek :empty @15:52:48.05 Stroke completed -- waiting for pressure to equalize ... @15:52:58.88 SS.reconfigure SSconfig @15:52:58.93 SS.hold @15:52:59.08 Intake.select :close @15:52:59.24 ShallowSampler::Clogged in WCR -- progress too slow after 825.0ml @15:52:59.26 SSV.dial :exhaust @15:53:00.09 Exhaust.select :open @15:53:00.20 SS.reconfigure SSexhale @15:53:00.83 SS.seek 0ml @15:53:10.15 Exhaust.select :close @15:53:10.27 Sampled 825.0ml @15:53:10.29 Drying puck before fixing for 4:00 @15:53:10.30 CBV.dial :waste2b @15:53:11.14 delay 1:00 @15:53:26.77 AFV.select :carrierSolution @15:53:26.89 delay 2 seconds @15:53:28.92 AS.reconfigure ASconfig @15:53:28.97 AS.seek 0.5ml @15:53:58.12 delay 1 second @15:53:59.13 AFV.select :holdingCoil @15:53:59.24 Building Slug @15:53:59.25 ATV.dial :ARV @15:54:00.91 delay 2 seconds @15:54:02.93 ARV.dial :air1 @15:54:03.34 delay 2 seconds @15:54:05.43 AS.reconfigure ASslow2 @15:54:05.48 AS.seek 0.506ml @15:54:11.16 CSV.dial :air @15:54:11.79 delay 2 seconds @15:54:11.81 CS.reconfigure CSfast @15:54:11.89 CS.seek 10ml @15:54:13.82 ARV.dial :MM1 @15:54:14.42 delay 2 seconds @15:54:16.45 Adding MM1 @15:54:16.53 AS.seek 0.524ml @15:54:35.02 delay 25 seconds @15:54:37.19 Pumping wcr puck from tube 4 up to 55psi... @15:54:37.20 CBV.dial SSV @15:54:38.62 CBV.dial between CSV and waste2b @15:54:39.68 CSV.dial between air and CTV @15:54:40.02 CS.seek 6.942ml @15:54:48.44 CTV.dial CSV,avoiding: :air @15:54:48.47 CSV.dial CTV @15:54:49.32 CS.reconfigure CSpressurize @15:54:49.38 CS.seek 0ml @15:55:00.04 ARV.dial :PP2 @15:55:00.44 Adding PP2 @15:55:00.45 delay 2 seconds @15:55:02.54 AS.seek 0.53ml @15:55:07.68 CSV.dial :air @15:55:08.19 CS.reconfigure CSfast @15:55:08.24 CS.seek 10ml @15:55:08.94 delay 10 seconds @15:55:18.96 ARV.dial :air2 @15:55:19.34 adding ARV air @15:55:19.35 delay 2 seconds @15:55:21.44 AS.seek 0.536ml @15:55:27.70 delay 5 seconds @15:55:32.72 ARV.dial :carrier @15:55:33.32 CSV.dial between air and CTV @15:55:33.67 CS.seek 2.569ml @15:55:33.97 delay 7 seconds @15:55:41.06 AS.seek 0.594ml @15:55:52.79 CTV.dial CSV,avoiding: :air @15:55:52.82 CSV.dial CTV @15:55:53.16 CS.reconfigure CSpressurize @15:55:53.22 CS.seek 0ml @15:55:58.21 CBV.dial :waste2b @15:55:58.58 CS.reconfigure CSconfig @15:55:58.63 CS.seek :empty @15:56:01.76 delay 1:00 @15:56:39.74 delay 5 seconds @15:56:44.76 ATV.dial :ASV @15:56:45.16 delay 2 seconds @15:56:47.25 AS.seek 0.6ml @15:56:53.52 delay 4 seconds @15:56:57.54 ATV.dial ARV @15:56:58.08 delay 2 seconds @15:57:00.10 ARV.dial :carrier @15:57:00.67 delay 2 seconds @15:57:01.79 CTV.dial :air,avoiding: :intake @15:57:02.77 AS.seek 0.65ml @15:57:03.23 Fix #1 of 2 (with rnal) for 2:00 @15:57:03.25 CSV.dial CSR @15:57:03.79 Pulling 2ml of CSR.rnal @15:57:03.81 CSR.select :rnal @15:57:03.93 CS.seek 2ml @15:57:10.04 delay 5 seconds @15:57:15.06 CSR.select :air @15:57:15.19 CS.reconfigure CSfast @15:57:15.24 CS.seek 10ml @15:57:35.35 Pumping wcr puck from tube 4 up to 55psi... @15:57:35.36 CBV.dial SSV @15:57:36.75 CBV.dial between CSV and waste2b @15:57:37.81 CSV.dial between air and CTV @15:57:38.47 CS.seek 6.894ml @15:57:46.95 CTV.dial CSV,avoiding: :air @15:57:46.98 CSV.dial CTV @15:57:48.46 CS.reconfigure CSpressurize @15:57:48.52 CS.seek 0ml @15:57:53.28 delay 4 seconds @15:57:57.30 ATV.dial PCR @15:57:57.89 ASV.dial :waste1 @15:57:58.67 ARV.dial :air1 @15:57:59.06 Positioning Slug by 0.324ml @15:57:59.15 AS.seek 0.326ml @15:58:06.73 CSV.dial :air @15:58:07.23 CS.reconfigure CSfast @15:58:07.29 CS.seek 10ml @15:58:32.32 CSV.dial between air and CTV @15:58:32.67 CS.seek 2.013ml @15:58:53.04 CTV.dial CSV,avoiding: :air @15:58:53.07 CSV.dial CTV @15:58:53.40 CS.reconfigure CSpressurize @15:58:53.46 CS.seek 0ml @15:58:54.75 delay 2:00 @16:00:54.77 CBV.dial :waste2b @16:00:55.14 CS.reconfigure CSconfig @16:00:55.19 CS.seek :empty @16:01:00.48 delay 1:00 @16:02:00.51 CTV.dial :air,avoiding: :intake @16:02:01.95 Fix #2 of 2 (with rnal) for 2:00 @16:02:01.97 CSV.dial CSR @16:02:02.51 Pulling 2ml of CSR.rnal @16:02:02.52 CSR.select :rnal @16:02:02.65 CS.seek 2ml @16:02:08.79 delay 5 seconds @16:02:13.81 CSR.select :air @16:02:13.94 CS.reconfigure CSfast @16:02:13.99 CS.seek 10ml @16:02:34.04 Pumping wcr puck from tube 4 up to 55psi... @16:02:34.05 CBV.dial SSV @16:02:35.46 CBV.dial between CSV and waste2b @16:02:36.53 CSV.dial between air and CTV @16:02:37.19 CS.seek 6.882ml @16:02:46.20 CTV.dial CSV,avoiding: :air @16:02:46.23 CSV.dial CTV @16:02:47.73 CS.reconfigure CSpressurize @16:02:47.79 CS.seek 0ml @16:03:06.00 CSV.dial :air @16:03:06.51 CS.reconfigure CSfast @16:03:06.57 CS.seek 10ml @16:03:25.39 delay 5 seconds @16:03:30.41 ATV.dial ARV @16:03:31.27 ASV.dial :PCRlock @16:03:31.66 CSV.dial between air and CTV @16:03:31.68 delay 5 seconds @16:03:32.05 CS.seek 2.002ml @16:03:36.74 Running Ss Profile60C350 thermocycling @16:03:36.76 PCR.program Profile60C350 @16:03:36.90 Profile60C350 should complete in < 1:48:05 @16:03:36.91 PCR.run 2:20:06.25 @16:03:52.45 CTV.dial CSV,avoiding: :air @16:03:52.48 CSV.dial CTV @16:03:52.81 CS.reconfigure CSpressurize @16:03:52.87 CS.seek 0ml @16:03:54.03 delay 2:00 @16:05:54.05 CBV.dial :waste2b @16:05:54.44 CS.reconfigure CSconfig @16:05:54.49 CS.seek :empty @16:05:59.98 delay 1:00 @16:07:00.00 CTV.dial :air,avoiding: :intake @16:07:01.51 Storing wcr puck in Collection Clamp to tube 3 @16:07:01.70 SC.reconfigure SCtight @16:07:01.75 SC.seek 3 @16:07:03.86 SC.reconfigure SCloose @16:07:04.09 Elbow.reconfigure TightElbow @16:07:04.14 Elbow.seek 3 @16:07:06.10 Forearm.reconfigure TightForearm @16:07:06.15 Forearm.seek 3 + 700 counts @16:07:07.34 SE.reconfigure SEpick @16:07:07.42 SE.seek beamBreak @16:07:10.33 SE.configure SEconfig @16:07:10.50 Elbow.reconfigure LimpElbow @16:07:10.55 Forearm.reconfigure LooseForearm @16:07:10.60 Forearm.seek 3 @16:07:10.94 Elbow.hold @16:07:11.02 Elbow.reconfigure TightElbow @16:07:11.13 SE.seek 2.06 Pucks below Top Plate @16:07:12.23 Forearm.force -20 @16:07:12.28 Forearm.seek 3 + 325 counts @16:07:12.61 Forearm.reconfigure TightForearm @16:07:12.67 Forearm.seek 3 + 700 counts @16:07:12.94 SE.seek 3.06 Pucks below Top Plate @16:07:13.53 Forearm.reconfigure LooseForearm @16:07:13.59 Forearm.seek :retract @16:07:14.52 Elbow.seek CC @16:07:16.27 Elbow.reconfigure LooseElbow @16:07:16.39 Forearm.seek CC @16:07:17.34 Hand.close @16:07:18.13 CC.seek :open @16:07:22.68 Forearm.seek :retract @16:07:23.66 Elbow.reconfigure TightElbow @16:07:23.71 Elbow.seek 3 @16:07:25.64 Elbow.reconfigure LimpElbow @16:07:25.70 Forearm.seek 3 @16:07:26.72 Elbow.hold @16:07:26.80 Elbow.reconfigure TightElbow @16:07:26.85 Hand.open @16:07:27.77 Forearm.force -20 @16:07:27.82 Forearm.seek 3 + 338 counts @16:07:28.16 Forearm.reconfigure TightForearm @16:07:28.21 Forearm.seek 3 + 700 counts @16:07:28.54 SE.seek :down @16:07:29.70 Forearm.reconfigure LooseForearm @16:07:29.75 Forearm.seek :retract @16:07:31.18 SE.coast @16:07:31.22 SC.coast @16:07:31.27 Starting finalPurge @16:07:31.28 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:07:31.36 Hand.open @16:07:31.77 Elbow.seek FlushPuck::Garage @16:07:33.79 Elbow.reconfigure LooseElbow @16:07:33.90 Forearm.seek :garage @16:07:34.88 Hand.close @16:07:35.64 Forearm.seek :retract @16:07:36.68 Elbow.reconfigure TightElbow @16:07:36.73 Elbow.seek CC @16:07:39.64 Elbow.reconfigure LooseElbow @16:07:39.75 Forearm.reconfigure TightForearm @16:07:40.39 Forearm.seek CC @16:07:41.62 CC.seek :closed @16:07:45.88 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.21 @16:07:45.97 Hand.open @16:07:46.83 Forearm.reconfigure LooseForearm @16:07:46.88 Forearm.seek :retract @16:07:47.85 Flushing the Collection Valve::Manifold @16:07:47.99 CSV.dial CSR @16:07:48.53 Pulling 5ml of CSR.flush @16:07:48.54 CSR.select :flush @16:07:48.67 CS.seek 5ml @16:08:02.52 delay 5 seconds @16:08:07.53 CSR.select :air @16:08:07.71 CS.reconfigure CSfast @16:08:07.76 CS.seek 7.5ml @16:08:14.94 CSV.dial :waste @16:08:16.36 CS.reconfigure CSconfig @16:08:16.41 CS.seek 0ml @16:08:36.49 Flushing lower manifold's waste1 port @16:08:36.63 CSV.dial :flush @16:08:37.11 CS.reconfigure CSfast @16:08:37.16 CS.seek 5ml @16:08:50.64 delay 5 seconds @16:08:55.65 CSV.dial :air @16:08:56.21 CS.seek 7ml @16:09:02.11 CSV.dial CTV @16:09:02.14
CTV.dial CSV @16:09:03.63 CBV.dial :waste1 @16:09:04.47 CS.seek 0ml @16:09:22.42 Flushing lower manifold's waste2b port @16:09:22.56 CSV.dial :flush @16:09:23.36 CS.seek 5ml @16:09:36.46 delay 5 seconds @16:09:41.47 CSV.dial :air @16:09:42.06 CS.seek 7.001ml @16:09:47.86 CSV.dial CTV @16:09:47.88
CTV.dial CSV @16:09:48.38 CBV.dial :waste2b @16:09:49.21 CS.seek 0ml @16:10:07.05 Flushing upper manifold @16:10:07.19 CSV.dial :flush @16:10:08.00 CS.seek 5ml @16:10:21.14 delay 5 seconds @16:10:26.15 CSV.dial :air @16:10:26.73 CS.seek 7ml @16:10:32.52 CSV.dial CBV @16:10:32.56
CBV.dial CSV @16:10:34.29 CTV.dial :air @16:10:35.76 CS.seek 0ml @16:10:53.43 delay 7 seconds @16:11:00.45 CS.seek 3ml @16:11:08.73 CSV.dial :waste @16:11:09.25 CS.seek 0ml @16:11:17.58 CSV.dial :flush @16:11:18.08 CS.seek 3ml @16:11:26.55 delay 5 seconds @16:11:31.56 CSV.dial :air @16:11:32.14 CS.seek 5ml @16:11:37.98 CSV.dial :air @16:11:38.01 CS.seek 0ml @16:11:51.05 delay 7 seconds @16:11:58.09 CS.seek 3ml @16:12:06.29 CSV.dial :waste @16:12:07.11 CS.seek 0ml @16:12:15.27 Disinfecting external Intake with mfbkill @16:12:15.40 CSV.dial CSR @16:12:16.88 Pulling 8ml of CSR.mfbkill @16:12:16.89 CSR.select :mfbkill @16:12:17.02 CS.seek 8ml @16:12:37.24 delay 5 seconds @16:12:42.26 CSR.select :air @16:12:42.40 CS.seek 10ml @16:12:48.24 CSV.dial CBV @16:12:48.27
CBV.dial CSV @16:12:49.38 CTV.dial :intake @16:12:50.19 Intake.select :open @16:12:50.31 CS.seek 2ml @16:13:11.17 Intake.select :close @16:13:11.29 CTV.dial :air @16:13:12.12 CS.reconfigure CSconfig @16:13:12.17 CS.seek 0ml @16:13:18.46 Flushing lower manifold's waste1 port @16:13:18.47 CSV.dial CSR @16:13:19.63 Pulling 4ml of CSR.mfbkill @16:13:19.64 CSR.select :mfbkill @16:13:19.77 CS.seek 4ml @16:13:31.18 delay 5 seconds @16:13:36.19 CSR.select :air @16:13:36.32 CS.reconfigure CSfast @16:13:36.37 CS.seek 10ml @16:13:51.83 CSV.dial CTV @16:13:51.86
CTV.dial CSV @16:13:53.38 CBV.dial :waste1 @16:13:53.89 CS.seek 0ml @16:14:19.02 Flushing lower manifold's waste2b port @16:14:19.03 CSV.dial CSR @16:14:19.58 Pulling 4ml of CSR.mfbkill @16:14:19.59 CSR.select :mfbkill @16:14:19.72 CS.reconfigure CSconfig @16:14:19.76 CS.seek 4ml @16:14:30.99 delay 5 seconds @16:14:36.00 CSR.select :air @16:14:36.13 CS.reconfigure CSfast @16:14:36.18 CS.seek 10ml @16:14:51.49 CSV.dial CTV @16:14:51.52
CTV.dial CSV @16:14:52.02 CBV.dial :waste2b @16:14:53.54 CS.seek 0ml @16:15:18.80 Killing upper manifold @16:15:18.81 CSV.dial CSR @16:15:19.36 Pulling 4ml of CSR.mfbkill @16:15:19.37 CSR.select :mfbkill @16:15:19.50 CS.reconfigure CSconfig @16:15:19.54 CS.seek 4ml @16:15:30.71 delay 5 seconds @16:15:35.73 CSR.select :air @16:15:35.86 CS.reconfigure CSfast @16:15:35.91 CS.seek 10ml @16:15:51.31 CSV.dial CBV @16:15:51.34
CBV.dial CSV @16:15:52.49 CTV.dial :air @16:15:53.94 CS.seek 0ml @16:16:19.09 delay 7 seconds @16:16:26.11 CS.seek 3ml @16:16:34.42 CSV.dial :waste @16:16:34.91 CS.seek 0ml @16:16:43.09 Flushing upper manifold @16:16:43.23 CSV.dial :flush @16:16:43.74 CS.seek 5ml @16:16:56.65 delay 5 seconds @16:17:01.66 CSV.dial :air @16:17:02.22 CS.seek 7ml @16:17:08.02 CSV.dial CBV @16:17:08.05
CBV.dial CSV @16:17:09.18 CTV.dial :air @16:17:09.20 CS.seek 0ml @16:17:26.90 delay 7 seconds @16:17:33.92 CS.seek 3ml @16:17:42.17 CSV.dial :waste @16:17:42.68 CS.seek 0ml @16:17:50.96 CSV.dial :flush @16:17:51.45 CS.seek 3ml @16:17:59.62 delay 5 seconds @16:18:04.63 CSV.dial :air @16:18:05.33 CS.seek 5ml @16:18:11.17 CSV.dial :air @16:18:11.20 CS.seek 0ml @16:18:24.18 delay 7 seconds @16:18:31.20 CS.seek 3ml @16:18:39.55 CSV.dial :waste @16:18:40.35 CS.seek 0ml @16:18:48.49 Flushing lower manifold's waste1 port @16:18:48.63 CSV.dial :flush @16:18:49.12 CS.seek 5ml @16:19:02.12 delay 5 seconds @16:19:07.13 CSV.dial :air @16:19:07.69 CS.seek 7.001ml @16:19:13.53 CSV.dial CTV @16:19:13.57
CTV.dial CSV @16:19:15.07 CBV.dial :waste1 @16:19:15.56 CS.seek 0ml @16:19:33.25 Flushing lower manifold's waste2b port @16:19:33.98 CSV.dial :flush @16:19:34.80 CS.seek 5ml @16:19:47.84 delay 5 seconds @16:19:52.85 CSV.dial :air @16:19:53.44 CS.seek 7.001ml @16:19:59.31 CSV.dial CTV @16:19:59.34
CTV.dial CSV @16:19:59.84 CBV.dial :waste2b @16:20:00.69 CS.seek 0ml @16:20:18.40 Disinfecting external Intake with kill @16:20:18.54 CSV.dial CSR @16:20:19.09 Pulling 8ml of CSR.kill @16:20:19.10 CSR.select :kill @16:20:19.23 CS.seek 8ml @16:20:39.28 delay 5 seconds @16:20:44.30 CSR.select :air @16:20:44.44 CS.seek 10ml @16:20:50.25 CSV.dial CBV @16:20:50.28
CBV.dial CSV @16:20:51.45 CTV.dial :intake @16:20:52.25 Intake.select :open @16:20:52.38 CS.seek 2ml @16:21:13.36 Intake.select :close @16:21:13.48 CTV.dial :air @16:21:14.28 CS.reconfigure CSconfig @16:21:14.33 CS.seek 0ml @16:21:20.36 CSV.dial CSR @16:21:21.54 Pulling 5ml of CSR.flush @16:21:21.55 CSR.select :flush @16:21:21.68 CS.seek 5ml @16:21:35.40 delay 5 seconds @16:21:40.42 CSR.select :air @16:21:40.55 CS.seek 10ml @16:21:54.33 CSV.dial CBV @16:21:55.45 CS.seek 0ml @16:22:22.06 Garaging FlushPuck in Collection Clamp @16:22:22.14 Elbow.reconfigure TightElbow @16:22:22.19 Elbow.seek CC @16:22:22.45 Elbow.reconfigure LooseElbow @16:22:22.56 Forearm.seek CC @16:22:23.56 Hand.close @16:22:24.28 CC.seek :open @16:22:28.69 Forearm.seek :retract @16:22:29.68 Elbow.reconfigure TightElbow @16:22:29.73 Elbow.seek :garage @16:22:32.84 Elbow.reconfigure LooseElbow @16:22:32.94 Forearm.reconfigure TightForearm @16:22:32.99 Forearm.seek :garage @16:22:34.16 Hand.open @16:22:34.97 Forearm.reconfigure LooseForearm @16:22:35.02 Forearm.seek :retract @16:22:36.07 Starting initialPurge @16:22:36.08 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:22:36.17 Hand.open @16:22:36.46 Elbow.reconfigure TightElbow @16:22:36.51 Elbow.seek FlushPuck::Garage @16:22:36.70 Elbow.reconfigure LooseElbow @16:22:37.39 Forearm.seek :garage @16:22:38.42 Hand.close @16:22:39.25 Forearm.seek :retract @16:22:40.23 Elbow.reconfigure TightElbow @16:22:40.28 Elbow.seek CC @16:22:43.10 Elbow.reconfigure LooseElbow @16:22:43.21 Forearm.reconfigure TightForearm @16:22:43.26 Forearm.seek CC @16:22:44.54 CC.seek :closed @16:22:48.79 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.19 @16:22:48.88 Hand.open @16:22:49.61 Forearm.reconfigure LooseForearm @16:22:49.65 Forearm.seek :retract @16:22:50.68 Flushing the Collection Valve::Manifold @16:22:50.82 CSV.dial CSR @16:22:52.00 Pulling 5ml of CSR.flush @16:22:52.01 CSR.select :flush @16:22:52.14 CS.seek 5ml @16:23:05.97 delay 5 seconds @16:23:10.99 CSR.select :air @16:23:11.16 CS.reconfigure CSfast @16:23:11.21 CS.seek 7.5ml @16:23:18.18 CSV.dial :waste @16:23:19.61 CS.reconfigure CSconfig @16:23:19.66 CS.seek 0ml @16:23:39.75 Purging external Intake @16:23:39.88 CSV.dial :flush @16:23:40.37 CS.reconfigure CSfast @16:23:40.42 CS.seek 10ml @16:24:05.22 delay 5 seconds @16:24:10.24 CSV.dial CBV @16:24:10.31
CBV.dial CSV @16:24:11.12 CTV.dial :intake @16:24:11.93 Intake.select :open @16:24:12.06 CS.seek 0ml @16:24:37.87 Intake.select :close @16:24:37.99 CTV.dial :air @16:24:38.81 Flushing upper manifold @16:24:38.94 CSV.dial :flush @16:24:39.75 CS.seek 5ml @16:24:52.69 delay 5 seconds @16:24:57.70 CSV.dial :air @16:24:58.27 CS.seek 7ml @16:25:04.00 CSV.dial CBV @16:25:04.02
CBV.dial CSV @16:25:05.16 CTV.dial :air @16:25:05.18 CS.seek 0ml @16:25:22.78 delay 7 seconds @16:25:29.81 CS.seek 3ml @16:25:38.02 CSV.dial :waste @16:25:38.52 CS.seek 0ml @16:25:46.75 CSV.dial :flush @16:25:47.26 CS.seek 3ml @16:25:55.41 delay 5 seconds @16:26:00.42 CSV.dial :air @16:26:00.98 CS.seek 5ml @16:26:06.68 CSV.dial :air @16:26:06.71 CS.seek 0ml @16:26:19.50 delay 7 seconds @16:26:26.52 CS.seek 3ml @16:26:34.62 CSV.dial :waste @16:26:35.45 CS.seek 0ml @16:26:43.53 Garaging FlushPuck in Collection Clamp @16:26:43.61 Elbow.reconfigure TightElbow @16:26:43.66 Elbow.seek CC @16:26:43.89 Elbow.reconfigure LooseElbow @16:26:44.00 Forearm.seek CC @16:26:45.01 Hand.close @16:26:45.78 CC.seek :open @16:26:50.16 Forearm.seek :retract @16:26:51.13 Elbow.reconfigure TightElbow @16:26:51.18 Elbow.seek :garage @16:26:54.25 Elbow.reconfigure LooseElbow @16:26:54.36 Forearm.reconfigure TightForearm @16:26:54.40 Forearm.seek :garage @16:26:55.58 Hand.open @16:26:56.35 Forearm.reconfigure LooseForearm @16:26:56.40 Forearm.seek :retract @16:26:57.43 Completed [mbaPhase] begun 14:00:26 @16:26:57.45 Preparing to sleep until Sun Oct 21 14:00:26 +0200 2018 @16:26:57.50 deferred until MFBPCR finish @16:26:57.52
Waiting for MFBPCR til 14:00CEST21-Oct-18 @16:26:57.54 Continuing at 14:00CEST21-Oct-18 @16:26:57.59 GoodNews.email "Then, in tube 4: mbaPhase\n/home/esp/esp2/mission/18tundeployv4.rb:127\n{1 => 22, 2 => 22, 3 => 4, 4 => 18}\nCan@16:26:57, 14.4C, 71% humidity, 14.3psia, 12.719V, 0.840A, 0.840A avg, 10.69W",Subject: "Waiting until 14:00CEST21-Oct-18" @17:26:24.32 PCR.off @17:26:26.35 PCR for Ss completed -- closed /var/log/esp/Ss18oct1915h300ml.pcr @17:26:26.42 Cleaning PCR @17:26:26.43 AFV.select :carrierSolution @17:26:26.55 delay 2 seconds @17:26:28.58 AS.reconfigure ASconfig @17:26:28.63 AS.seek 0.5ml @17:26:38.92 delay 1 second @17:26:39.93 AFV.select :holdingCoil @17:26:40.04 ASV.dial :waste1 @17:26:40.42 ATV.dial :air3 @17:26:41.63 AS.reconfigure ASslow1 @17:26:41.68 AS.seek 0.51ml @17:26:43.95 delay 2 seconds @17:26:45.97 ATV.dial :bleach @17:26:46.58 AS.seek 0.81ml @17:27:47.17 delay 3 seconds @17:27:50.19 ATV.dial :air3 @17:27:50.73 delay 3 seconds @17:27:53.82 AS.seek 0.835ml @17:27:59.11 delay 3 seconds @17:28:02.13 ATV.dial :bleach @17:28:02.74 AS.seek 0.82ml @17:28:06.03 delay 3 seconds @17:28:09.05 ARV.dial :waste @17:28:09.64 delay 3 seconds @17:28:13.17 ATV.dial :ARV @17:28:14.10 AS.reconfigure ASconfig @17:28:14.15 AS.seek 0.47ml @17:28:34.56 AFV.select :carrierSolution @17:28:34.67 delay 2 seconds @17:28:36.70 AS.seek 1ml @17:29:07.45 delay 1 second @17:29:08.46 AFV.select :holdingCoil @17:29:08.57 delay 1 second @17:29:09.60 AS.reconfigure ASfast @17:29:09.65 AS.seek 0ml @17:29:36.99 AFV.select :carrierSolution @17:29:37.11 delay 2 seconds @17:29:39.14 AS.reconfigure ASconfig @17:29:39.18 AS.seek 1ml @17:30:36.98 delay 1 second @17:30:37.99 AFV.select :holdingCoil @17:30:38.10 delay 1 second @17:30:39.13 AS.reconfigure ASfast @17:30:39.18 AS.seek 0ml @17:31:06.50 AFV.select :carrierSolution @17:31:06.62 delay 2 seconds @17:31:08.65 AS.reconfigure ASconfig @17:31:08.70 AS.seek 1ml @17:32:06.50 delay 1 second @17:32:07.51 AFV.select :holdingCoil @17:32:07.62 delay 1 second @17:32:08.65 AS.reconfigure ASfast @17:32:08.70 AS.seek 0ml @17:32:36.04 ARV.dial between carrier and air1 @17:32:36.53 ATV.dial :air3 @17:32:37.76 AS.reconfigure ASslow1 @17:32:37.81 AS.seek 0.01ml @17:32:40.17 delay 2 seconds @17:32:42.18 ATV.dial :bleach @17:32:42.80 AS.seek 0.81ml @17:35:23.94 delay 3 seconds @17:35:26.96 ATV.dial :air3 @17:35:27.50 delay 3 seconds @17:35:30.59 AS.seek 0.835ml @17:35:35.93 delay 3 seconds @17:35:38.95 ATV.dial :bleach @17:35:39.57 AS.seek 0.82ml @17:35:42.86 delay 3 seconds @17:35:45.88 ATV.dial PCR @17:35:46.37 AS.reconfigure ASconfig @17:35:46.42 AS.seek :empty @17:36:33.88 PCR.program :bleachProfile @17:36:33.95 PCR program should complete in < 2:45 @17:36:33.96 PCR.run 10:00 @17:39:44.87 PCR.off @17:39:44.90 AFV.select :carrierSolution @17:39:45.01 delay 2 seconds @17:39:47.04 AS.seek 1ml @17:40:44.86 delay 1 second @17:40:45.87 AFV.select :holdingCoil @17:40:45.98 delay 1 second @17:40:47.01 AS.reconfigure ASfast @17:40:47.06 AS.seek 0ml @17:41:14.41 delay 3 seconds @17:41:17.43 AFV.select :carrierSolution @17:41:17.56 delay 2 seconds @17:41:19.59 AS.reconfigure ASconfig @17:41:19.64 AS.seek 1ml @17:42:17.39 delay 1 second @17:42:18.40 AFV.select :holdingCoil @17:42:18.51 delay 1 second @17:42:19.54 AS.reconfigure ASfast @17:42:19.59 AS.seek 0ml @17:42:46.92 delay 3 seconds @17:42:49.94 AFV.select :carrierSolution @17:42:50.05 delay 2 seconds @17:42:52.08 AS.reconfigure ASconfig @17:42:52.13 AS.seek 1ml @17:43:49.94 delay 1 second @17:43:50.95 AFV.select :holdingCoil @17:43:51.06 delay 1 second @17:43:52.09 AS.reconfigure ASfast @17:43:52.14 AS.seek 0ml @17:44:19.49 delay 3 seconds @17:44:22.50 AFV.select :carrierSolution @17:44:22.61 delay 2 seconds @17:44:24.64 AS.reconfigure ASconfig @17:44:24.69 AS.seek 1ml @17:45:22.47 delay 1 second @17:45:23.48 AFV.select :holdingCoil @17:45:23.59 delay 1 second @17:45:24.62 AS.reconfigure ASfast @17:45:24.67 AS.seek 0ml @17:45:52.00 delay 3 seconds @17:45:55.02 AFV.select :carrierSolution @17:45:55.13 delay 2 seconds @17:45:57.16 AS.reconfigure ASconfig @17:45:57.22 AS.seek 1ml @17:46:54.99 delay 1 second @17:46:56.01 AFV.select :holdingCoil @17:46:56.12 delay 1 second @17:46:57.15 AS.reconfigure ASnorm @17:46:57.20 AS.seek 0ml @17:47:47.90 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct1917h300ml.pcr @17:47:47.92 Building PCR Slug from ASV with MM2 mix, PP4 primer @17:47:48.50 Priming with carrier @17:47:48.54 ATV.dial ARV @17:47:49.22 ARV.dial :carrier @17:47:49.57 AS.seek 0.5ml @17:48:15.12 AFV.select :carrierSolution @17:48:15.24 delay 2 seconds @17:48:17.27 AS.reconfigure ASconfig @17:48:17.32 AS.seek 1ml @17:48:46.35 delay 1 second @17:48:47.36 AFV.select :holdingCoil @17:48:47.47 delay 1 second @17:48:48.49 ATV.dial :waste @17:48:50.05 AS.reconfigure ASnorm @17:48:50.10 AS.seek :empty @17:49:40.87 AFV.select :carrierSolution @17:49:40.99 delay 2 seconds @17:49:43.02 AS.reconfigure ASconfig @17:49:43.07 AS.seek 0.5ml @17:50:12.18 delay 1 second @17:50:13.19 AFV.select :holdingCoil @17:50:13.30 Building Slug @17:50:13.31 ATV.dial :ARV @17:50:14.85 delay 2 seconds @17:50:16.87 ARV.dial :air1 @17:50:17.26 delay 2 seconds @17:50:19.36 AS.reconfigure ASslow2 @17:50:19.41 AS.seek 0.506ml @17:50:25.66 delay 2 seconds @17:50:27.67 ARV.dial :MM2 @17:50:29.10 delay 2 seconds @17:50:31.13 Adding MM2 @17:50:31.20 AS.seek 0.524ml @17:50:49.68 delay 25 seconds @17:51:14.70 ARV.dial :PP4 @17:51:15.10 Adding PP4 @17:51:15.11 delay 2 seconds @17:51:17.20 AS.seek 0.53ml @17:51:23.44 delay 10 seconds @17:51:33.46 ARV.dial :air3 @17:51:33.85 adding ARV air @17:51:33.86 delay 2 seconds @17:51:35.95 AS.seek 0.536ml @17:51:42.29 delay 5 seconds @17:51:47.31 ARV.dial :carrier @17:51:48.52 delay 7 seconds @17:51:55.61 AS.seek 0.594ml @17:52:54.36 delay 5 seconds @17:52:59.38 ATV.dial :ASV @17:52:59.79 delay 2 seconds @17:53:01.88 AS.seek 0.6ml @17:53:08.19 delay 4 seconds @17:53:12.21 ATV.dial ARV @17:53:12.74 delay 2 seconds @17:53:14.76 ARV.dial :carrier @17:53:14.78 delay 2 seconds @17:53:16.87 AS.seek 0.65ml @17:54:07.38 delay 4 seconds @17:54:11.40 ATV.dial PCR @17:54:11.99 ASV.dial :waste1 @17:54:12.00 ARV.dial :air1 @17:54:12.36 Positioning Slug by 0.324ml @17:54:12.46 AS.seek 0.326ml @17:59:38.34 delay 5 seconds @17:59:43.36 ATV.dial ARV @17:59:44.21 ASV.dial :PCRlock @17:59:44.63 delay 5 seconds @17:59:49.66 Running Tt1 Profile60C300 thermocycling @17:59:49.67 PCR.program Profile60C300 @17:59:49.81 Profile60C300 should complete in < 1:45:21 @17:59:49.82 PCR.run 2:16:41.25 @19:21:47.94 PCR.off @19:21:49.50 PCR for Tt1 completed -- closed /var/log/esp/Tt118oct1917h300ml.pcr @19:21:49.57 Cleaning PCR @19:21:49.59 AFV.select :carrierSolution @19:21:49.70 delay 2 seconds @19:21:51.73 AS.reconfigure ASconfig @19:21:51.78 AS.seek 0.5ml @19:22:02.07 delay 1 second @19:22:03.09 AFV.select :holdingCoil @19:22:03.20 ASV.dial :waste1 @19:22:03.58 ATV.dial :air3 @19:22:04.80 AS.reconfigure ASslow1 @19:22:04.85 AS.seek 0.51ml @19:22:07.16 delay 2 seconds @19:22:09.18 ATV.dial :bleach @19:22:09.79 AS.seek 0.81ml @19:23:10.58 delay 3 seconds @19:23:13.75 ATV.dial :air3 @19:23:14.72 delay 3 seconds @19:23:18.37 AS.seek 0.835ml @19:23:23.99 delay 3 seconds @19:23:27.04 ATV.dial :bleach @19:23:27.65 AS.seek 0.82ml @19:23:30.94 delay 3 seconds @19:23:33.96 ARV.dial :waste @19:23:35.18 delay 3 seconds @19:23:38.20 ATV.dial :ARV @19:23:39.07 AS.reconfigure ASconfig @19:23:39.12 AS.seek 0.47ml @19:23:59.49 AFV.select :carrierSolution @19:23:59.61 delay 2 seconds @19:24:01.64 AS.seek 1ml @19:24:32.44 delay 1 second @19:24:33.45 AFV.select :holdingCoil @19:24:33.56 delay 1 second @19:24:34.59 AS.reconfigure ASfast @19:24:34.64 AS.seek 0ml @19:25:01.98 AFV.select :carrierSolution @19:25:02.10 delay 2 seconds @19:25:04.13 AS.reconfigure ASconfig @19:25:04.18 AS.seek 1ml @19:26:01.97 delay 1 second @19:26:02.98 AFV.select :holdingCoil @19:26:03.09 delay 1 second @19:26:04.12 AS.reconfigure ASfast @19:26:04.17 AS.seek 0ml @19:26:31.50 AFV.select :carrierSolution @19:26:31.62 delay 2 seconds @19:26:33.65 AS.reconfigure ASconfig @19:26:33.70 AS.seek 1ml @19:27:31.49 delay 1 second @19:27:32.50 AFV.select :holdingCoil @19:27:32.61 delay 1 second @19:27:33.64 AS.reconfigure ASfast @19:27:33.69 AS.seek 0ml @19:28:01.04 ARV.dial between carrier and air1 @19:28:01.52 ATV.dial :air3 @19:28:02.76 AS.reconfigure ASslow1 @19:28:02.81 AS.seek 0.01ml @19:28:05.16 delay 2 seconds @19:28:07.17 ATV.dial :bleach @19:28:07.78 AS.seek 0.81ml @19:30:48.93 delay 3 seconds @19:30:51.95 ATV.dial :air3 @19:30:52.48 delay 3 seconds @19:30:55.57 AS.seek 0.835ml @19:31:00.94 delay 3 seconds @19:31:03.96 ATV.dial :bleach @19:31:04.56 AS.seek 0.82ml @19:31:07.83 delay 3 seconds @19:31:10.86 ATV.dial PCR @19:31:11.27 AS.reconfigure ASconfig @19:31:11.32 AS.seek :empty @19:31:58.80 PCR.program :bleachProfile @19:31:58.89 PCR program should complete in < 2:45 @19:31:58.91 PCR.run 10:00 @19:35:09.84 PCR.off @19:35:09.90 AFV.select :carrierSolution @19:35:10.07 delay 2 seconds @19:35:12.10 AS.seek 1ml @19:36:09.93 delay 1 second @19:36:10.95 AFV.select :holdingCoil @19:36:11.06 delay 1 second @19:36:12.09 AS.reconfigure ASfast @19:36:12.14 AS.seek 0ml @19:36:39.48 delay 3 seconds @19:36:42.50 AFV.select :carrierSolution @19:36:42.61 delay 2 seconds @19:36:44.64 AS.reconfigure ASconfig @19:36:44.70 AS.seek 1ml @19:37:42.54 delay 1 second @19:37:43.55 AFV.select :holdingCoil @19:37:43.66 delay 1 second @19:37:44.69 AS.reconfigure ASfast @19:37:44.74 AS.seek 0ml @19:38:12.09 delay 3 seconds @19:38:15.11 AFV.select :carrierSolution @19:38:15.22 delay 2 seconds @19:38:17.25 AS.reconfigure ASconfig @19:38:17.31 AS.seek 1ml @19:39:15.07 delay 1 second @19:39:16.08 AFV.select :holdingCoil @19:39:16.19 delay 1 second @19:39:17.22 AS.reconfigure ASfast @19:39:17.27 AS.seek 0ml @19:39:44.60 delay 3 seconds @19:39:47.62 AFV.select :carrierSolution @19:39:47.73 delay 2 seconds @19:39:49.76 AS.reconfigure ASconfig @19:39:49.82 AS.seek 1ml @19:40:47.62 delay 1 second @19:40:48.63 AFV.select :holdingCoil @19:40:48.74 delay 1 second @19:40:49.77 AS.reconfigure ASfast @19:40:49.82 AS.seek 0ml @19:41:17.17 delay 3 seconds @19:41:20.19 AFV.select :carrierSolution @19:41:20.30 delay 2 seconds @19:41:22.33 AS.reconfigure ASconfig @19:41:22.39 AS.seek 1ml @19:42:20.18 delay 1 second @19:42:21.19 AFV.select :holdingCoil @19:42:21.30 delay 1 second @19:42:22.33 AS.reconfigure ASnorm @19:42:22.38 AS.seek 0ml @19:43:13.08 Setting up PCR for IPc -- writing /var/log/esp/IPc18oct1919h300ml.pcr @19:43:13.69 Building PCR Slug from ASV with MM1 mix, PP1 primer @19:43:13.71 Priming with carrier @19:43:13.72 ATV.dial ARV @19:43:14.40 ARV.dial :carrier @19:43:14.75 AS.seek 0.5ml @19:43:40.38 AFV.select :carrierSolution @19:43:40.50 delay 2 seconds @19:43:42.53 AS.reconfigure ASconfig @19:43:42.58 AS.seek 1ml @19:44:11.58 delay 1 second @19:44:12.60 AFV.select :holdingCoil @19:44:12.71 delay 1 second @19:44:13.73 ATV.dial :waste @19:44:15.29 AS.reconfigure ASnorm @19:44:15.34 AS.seek :empty @19:45:06.08 AFV.select :carrierSolution @19:45:06.20 delay 2 seconds @19:45:08.23 AS.reconfigure ASconfig @19:45:08.28 AS.seek 0.5ml @19:45:37.39 delay 1 second @19:45:38.40 AFV.select :holdingCoil @19:45:38.51 Building Slug @19:45:38.52 ATV.dial :ARV @19:45:40.06 delay 2 seconds @19:45:42.08 ARV.dial :air1 @19:45:42.48 delay 2 seconds @19:45:44.56 AS.reconfigure ASslow2 @19:45:44.62 AS.seek 0.506ml @19:45:50.89 delay 2 seconds @19:45:52.91 ARV.dial :MM1 @19:45:53.50 delay 2 seconds @19:45:55.53 Adding MM1 @19:45:55.60 AS.seek 0.524ml @19:46:13.89 delay 25 seconds @19:46:38.91 ARV.dial :PP1 @19:46:39.31 Adding PP1 @19:46:39.32 delay 2 seconds @19:46:41.41 AS.seek 0.53ml @19:46:47.86 delay 10 seconds @19:46:57.88 ARV.dial :air1 @19:46:58.26 adding ARV air @19:46:58.27 delay 2 seconds @19:47:00.36 AS.seek 0.536ml @19:47:06.78 delay 5 seconds @19:47:11.80 ARV.dial :carrier @19:47:12.20 delay 7 seconds @19:47:19.29 AS.seek 0.594ml @19:48:17.92 delay 5 seconds @19:48:22.95 ATV.dial :ASV @19:48:23.34 delay 2 seconds @19:48:25.43 AS.seek 0.6ml @19:48:31.79 delay 4 seconds @19:48:35.82 ATV.dial ARV @19:48:36.34 delay 2 seconds @19:48:38.36 ARV.dial :carrier @19:48:38.38 delay 2 seconds @19:48:40.46 AS.seek 0.65ml @19:49:31.06 delay 4 seconds @19:49:35.08 ATV.dial PCR @19:49:35.66 ASV.dial :waste1 @19:49:35.68 ARV.dial :air1 @19:49:36.07 Positioning Slug by 0.324ml @19:49:36.16 AS.seek 0.326ml @19:55:02.12 delay 5 seconds @19:55:07.14 ATV.dial ARV @19:55:07.89 ASV.dial :PCRlock @19:55:08.36 delay 5 seconds @19:55:13.39 Running IPc ProfileIPc thermocycling @19:55:13.40 PCR.program ProfileIPc @19:55:13.56 ProfileIPc should complete in < 1:50:49 @19:55:13.57 PCR.run 2:23:31.25 @21:19:31.12 PCR.off @21:19:34.29 PCR for IPc completed -- closed /var/log/esp/IPc18oct1919h300ml.pcr @21:19:34.30 Full MFB Decontamination @21:19:34.31 Decon extraction column and decon AIV @21:19:34.34 SPE.hold 2:46:40,55C @21:19:34.38 ASV.dial :SPEdelivery @21:19:35.01 delay 20 seconds @21:19:55.04 AFV.select :carrierSolution @21:19:55.15 delay 2 seconds @21:19:57.18 AS.reconfigure ASconfig @21:19:57.23 AS.seek 0.5ml @21:20:07.55 delay 1 second @21:20:08.56 AFV.select :holdingCoil @21:20:08.88 ATV.dial :air1 @21:20:10.69 AS.reconfigure ASslow1 @21:20:10.74 AS.seek 0.52ml @21:20:15.05 ATV.dial :ASV @21:20:16.75 ASV.dial :SPErecovery @21:20:17.41 AS.seek 0.62ml @21:20:37.79 ATV.dial :waste @21:20:39.70 AS.reconfigure ASnorm @21:20:39.75 AS.seek :empty @21:21:11.38 AFV.select :holdingCoil @21:21:11.40 ATV.dial :waste @21:21:11.42 AS.reconfigure ASconfig @21:21:11.47 AS.seek 0ml @21:21:11.74 AFV.select :carrierSolution @21:21:11.86 delay 2 seconds @21:21:13.89 AS.seek 0.5ml @21:21:42.91 delay 1 second @21:21:43.92 AFV.select :holdingCoil @21:21:44.03 ATV.dial :air3 @21:21:44.83 AS.reconfigure ASslow1 @21:21:44.88 AS.seek 0.52ml @21:21:49.25 ATV.dial :air3 @21:21:49.35 AS.seek 0.53ml @21:21:51.64 delay 2 seconds @21:21:53.65 ATV.dial :bleach @21:21:54.15 AS.seek 0.71ml @21:22:30.67 delay 3 seconds @21:22:33.69 ATV.dial :air3 @21:22:34.25 delay 3 seconds @21:22:37.34 AS.seek 0.735ml @21:22:42.62 delay 3 seconds @21:22:45.64 ATV.dial :bleach @21:22:46.27 AS.seek 0.72ml @21:22:49.56 delay 3 seconds @21:22:52.59 ATV.dial ASV @21:22:53.62 ASV.dial :SPErecovery @21:22:53.71 AS.seek 0.365ml @21:24:05.38 ATV.dial :waste @21:24:07.06 AS.reconfigure ASnorm @21:24:07.11 AS.seek :empty @21:24:25.88 ATV.dial :air3 @21:24:26.58 AS.reconfigure ASslow1 @21:24:26.63 AS.seek 0.009ml @21:24:28.94 delay 2 seconds @21:24:30.95 ATV.dial :bleach @21:24:31.43 AS.seek 0.579ml @21:26:26.34 delay 3 seconds @21:26:29.36 ATV.dial :air3 @21:26:29.91 delay 3 seconds @21:26:33.00 AS.seek 0.604ml @21:26:38.29 delay 3 seconds @21:26:41.31 ATV.dial :bleach @21:26:41.93 AS.seek 0.589ml @21:26:45.23 delay 3 seconds @21:26:48.25 ATV.dial :ASV @21:26:49.27 ASV.dial :wasteR @21:26:49.75 AS.seek 0ml @21:28:48.52 delay 1:00 @21:29:48.54 ATV.dial ASV @21:29:48.56 AFV.select :carrierSolution @21:29:48.66 delay 2 seconds @21:29:50.69 AS.reconfigure ASconfig @21:29:50.74 AS.seek 1ml @21:30:48.55 delay 1 second @21:30:49.56 AFV.select :holdingCoil @21:30:49.67 delay 1 second @21:30:50.69 ASV.dial :wasteR @21:30:50.71 AS.reconfigure ASslow1 @21:30:51.34 AS.seek 0ml @21:34:12.74 delay 10 seconds @21:34:22.76 ATV.dial :air3 @21:34:24.21 AS.seek 0.01ml @21:34:26.54 delay 2 seconds @21:34:28.56 ATV.dial :bleach @21:34:29.20 AS.seek 0.58ml @21:36:24.10 delay 3 seconds @21:36:27.12 ATV.dial :air3 @21:36:27.66 delay 3 seconds @21:36:30.75 AS.seek 0.605ml @21:36:36.03 delay 3 seconds @21:36:39.05 ATV.dial :bleach @21:36:39.67 AS.seek 0.59ml @21:36:42.99 delay 3 seconds @21:36:46.02 ATV.dial ASV @21:36:47.13 ASV.dial :SPEdelivery @21:36:47.51 AS.seek 0ml @21:38:46.38 delay 1:00 @21:39:46.40 AFV.select :carrierSolution @21:39:46.51 delay 2 seconds @21:39:48.54 AS.reconfigure ASconfig @21:39:48.59 AS.seek 1ml @21:40:46.38 delay 1 second @21:40:47.40 AFV.select :holdingCoil @21:40:47.51 delay 1 second @21:40:48.53 ATV.dial ASV @21:40:48.55 ASV.dial :SPEdelivery @21:40:48.57 AS.reconfigure ASslow1 @21:40:48.61 AS.seek 0ml @21:44:09.97 delay 10 seconds @21:44:19.99 ATV.dial :air2 @21:44:20.80 AS.reconfigure ASslow0 @21:44:20.86 AS.seek 0.8ml @21:45:41.68 delay 3 seconds @21:45:44.70 ATV.dial :ASV @21:45:45.66 ASV.dial :SPEdelivery @21:45:45.68 AS.reconfigure ASslow1 @21:45:45.73 AS.seek 0.03ml @21:48:20.90 delay 20 seconds @21:48:40.93 ATV.dial :waste,via: :air3 @21:48:42.57 AS.reconfigure ASconfig @21:48:42.62 AS.seek :empty @21:48:44.68 SPE.coast @21:48:44.77 Removing Bleach from Mixing Coil @21:48:44.79 AFV.select :carrierSolution @21:48:44.89 delay 2 seconds @21:48:46.92 AS.seek 0.5ml @21:49:16.02 delay 1 second @21:49:17.03 AFV.select :holdingCoil @21:49:17.14 ATV.dial :ASV @21:49:18.76 ASV.dial :SPErecovery @21:49:19.39 AS.reconfigure ASslow1 @21:49:19.44 AS.seek 0.73ml @21:50:05.97 ASV.dial :wasteD @21:50:06.55 AS.reconfigure ASnorm @21:50:06.60 AS.seek 0ml @21:50:43.72 AFV.select :carrierSolution @21:50:43.84 delay 2 seconds @21:50:45.87 AS.reconfigure ASconfig @21:50:45.92 AS.seek 0.5ml @21:51:14.99 delay 1 second @21:51:16.00 AFV.select :holdingCoil @21:51:16.11 ATV.dial ASV @21:51:16.12 ASV.dial :SPErecovery @21:51:16.77 AS.reconfigure ASslow0 @21:51:16.82 AS.seek 0.3ml @21:51:37.23 ATV.dial :air2 @21:51:38.23 AS.reconfigure ASslow1 @21:51:38.28 AS.seek 0.32ml @21:51:42.64 ATV.dial ASV @21:51:43.64 AS.reconfigure ASslow0 @21:51:43.69 AS.seek 0.57ml @21:52:09.14 ASV.dial :wasteD @21:52:09.82 AS.reconfigure ASnorm @21:52:09.87 AS.seek 0ml @21:52:38.94 AFV.select :carrierSolution @21:52:39.06 delay 2 seconds @21:52:41.09 AS.reconfigure ASconfig @21:52:41.14 AS.seek 0.5ml @21:53:10.20 delay 1 second @21:53:11.21 AFV.select :holdingCoil @21:53:11.32 ATV.dial ASV @21:53:11.33 ASV.dial :SPErecovery @21:53:11.95 AS.reconfigure ASslow0 @21:53:12.00 AS.seek 0.3ml @21:53:32.44 ATV.dial :air2 @21:53:33.45 AS.reconfigure ASslow1 @21:53:33.50 AS.seek 0.32ml @21:53:37.83 ATV.dial ASV @21:53:39.42 AS.reconfigure ASslow0 @21:53:39.48 AS.seek 0.57ml @21:54:04.94 ASV.dial :wasteD @21:54:05.60 AS.reconfigure ASnorm @21:54:05.66 AS.seek 0ml @21:54:34.76 ATV.dial :air2 @21:54:35.70 AS.reconfigure ASslow0 @21:54:35.75 AS.seek 0.3ml @21:55:06.22 ATV.dial :ASV @21:55:07.17 ASV.dial :wasteD @21:55:07.19 AS.reconfigure ASconfig @21:55:07.24 AS.seek 0.05ml @21:55:21.95 ATV.dial :waste @21:55:23.72 AS.seek 0ml @21:55:26.89 ASV.dial :SPEdelivery @21:55:27.85 deconLysateINT @21:55:27.86 ATV.dial :air3 @21:55:28.52 AS.reconfigure ASslow1 @21:55:28.57 AS.seek 0.01ml @21:55:30.90 delay 2 seconds @21:55:32.92 ATV.dial :bleach @21:55:33.39 AS.seek 0.96ml @21:58:44.70 delay 3 seconds @21:58:47.72 ATV.dial :air3 @21:58:48.24 delay 3 seconds @21:58:51.33 AS.seek 0.985ml @21:58:56.63 delay 3 seconds @21:58:59.65 ATV.dial :bleach @21:59:00.27 AS.seek 0.97ml @21:59:03.59 delay 3 seconds @21:59:06.62 ATV.dial AIV @21:59:07.92 AIV.dial :coilToMFB @21:59:07.93 AWV.select :waste @21:59:07.95 AFV.select :carrierSolution @21:59:08.06 delay 2 seconds @21:59:10.09 AS.reconfigure ASconfig @21:59:10.14 AS.seek 1ml @21:59:12.18 delay 1 second @21:59:13.19 AFV.select :holdingCoil @21:59:13.30 delay 1 second @21:59:14.33 AS.reconfigure ASslow0 @21:59:14.38 AS.seek :empty @22:00:55.30 AFV.select :carrierSolution @22:00:55.42 delay 2 seconds @22:00:57.45 AS.reconfigure ASconfig @22:00:57.50 AS.seek 1ml @22:01:55.31 delay 1 second @22:01:56.33 AFV.select :holdingCoil @22:01:56.44 delay 1 second @22:01:57.47 AS.reconfigure ASslow0 @22:01:57.52 AS.seek :empty @22:03:38.47 AFV.select :carrierSolution @22:03:38.59 delay 2 seconds @22:03:40.62 AS.reconfigure ASconfig @22:03:40.67 AS.seek 1ml @22:04:38.48 delay 1 second @22:04:39.50 AFV.select :holdingCoil @22:04:39.61 delay 1 second @22:04:40.64 AS.reconfigure ASslow0 @22:04:40.69 AS.seek :empty @22:06:21.66 ATV.dial :air2 @22:06:22.33 AS.reconfigure ASslow1 @22:06:22.38 AS.seek 0.009ml @22:06:24.75 AS.reconfigure ASslow0 @22:06:24.80 AS.seek 1ml @22:08:04.78 ATV.dial AIV @22:08:05.52 AS.seek 0.02ml @22:09:44.35 ATV.dial :waste @22:09:46.02 AS.seek 0ml @22:09:48.33 AWV.select :air @22:09:48.50 Cleaning PCR @22:09:48.51 AFV.select :carrierSolution @22:09:48.62 delay 2 seconds @22:09:50.65 AS.reconfigure ASconfig @22:09:50.70 AS.seek 0.5ml @22:10:19.73 delay 1 second @22:10:20.74 AFV.select :holdingCoil @22:10:20.85 ASV.dial :waste1 @22:10:21.22 ATV.dial :air3 @22:10:21.99 AS.reconfigure ASslow1 @22:10:22.04 AS.seek 0.51ml @22:10:24.39 delay 2 seconds @22:10:26.40 ATV.dial :bleach @22:10:26.97 AS.seek 0.81ml @22:11:27.53 delay 3 seconds @22:11:30.55 ATV.dial :air3 @22:11:31.08 delay 3 seconds @22:11:34.17 AS.seek 0.835ml @22:11:39.46 delay 3 seconds @22:11:42.48 ATV.dial :bleach @22:11:43.85 AS.seek 0.82ml @22:11:47.15 delay 3 seconds @22:11:50.17 ARV.dial :waste @22:11:50.76 delay 3 seconds @22:11:53.78 ATV.dial :ARV @22:11:54.65 AS.reconfigure ASconfig @22:11:54.70 AS.seek 0.47ml @22:12:15.11 AFV.select :carrierSolution @22:12:15.23 delay 2 seconds @22:12:17.26 AS.seek 1ml @22:12:48.01 delay 1 second @22:12:49.03 AFV.select :holdingCoil @22:12:49.14 delay 1 second @22:12:50.17 AS.reconfigure ASfast @22:12:50.22 AS.seek 0ml @22:13:17.66 AFV.select :carrierSolution @22:13:17.78 delay 2 seconds @22:13:19.81 AS.reconfigure ASconfig @22:13:19.86 AS.seek 1ml @22:14:17.65 delay 1 second @22:14:18.66 AFV.select :holdingCoil @22:14:18.77 delay 1 second @22:14:19.80 AS.reconfigure ASfast @22:14:19.85 AS.seek 0ml @22:14:47.19 AFV.select :carrierSolution @22:14:47.31 delay 2 seconds @22:14:49.34 AS.reconfigure ASconfig @22:14:49.39 AS.seek 1ml @22:15:47.19 delay 1 second @22:15:48.20 AFV.select :holdingCoil @22:15:48.31 delay 1 second @22:15:49.34 AS.reconfigure ASfast @22:15:49.39 AS.seek 0ml @22:16:16.72 ARV.dial between carrier and air1 @22:16:17.20 ATV.dial :air3 @22:16:18.44 AS.reconfigure ASslow1 @22:16:18.49 AS.seek 0.01ml @22:16:20.83 delay 2 seconds @22:16:22.85 ATV.dial :bleach @22:16:23.46 AS.seek 0.81ml @22:19:04.63 delay 3 seconds @22:19:07.65 ATV.dial :air3 @22:19:08.19 delay 3 seconds @22:19:11.28 AS.seek 0.835ml @22:19:16.64 delay 3 seconds @22:19:19.66 ATV.dial :bleach @22:19:20.26 AS.seek 0.82ml @22:19:23.52 delay 3 seconds @22:19:26.54 ATV.dial PCR @22:19:26.95 AS.reconfigure ASconfig @22:19:27.00 AS.seek :empty @22:20:14.44 PCR.program :bleachProfile @22:20:14.51 PCR program should complete in < 2:45 @22:20:14.52 PCR.run 10:00 @22:23:34.52 PCR.off @22:23:34.54 AFV.select :carrierSolution @22:23:34.66 delay 2 seconds @22:23:36.69 AS.seek 1ml @22:24:34.52 delay 1 second @22:24:35.53 AFV.select :holdingCoil @22:24:35.64 delay 1 second @22:24:36.67 AS.reconfigure ASfast @22:24:36.72 AS.seek 0ml @22:25:04.06 delay 3 seconds @22:25:07.08 AFV.select :carrierSolution @22:25:07.19 delay 2 seconds @22:25:09.22 AS.reconfigure ASconfig @22:25:09.28 AS.seek 1ml @22:26:07.06 delay 1 second @22:26:08.07 AFV.select :holdingCoil @22:26:08.18 delay 1 second @22:26:09.21 AS.reconfigure ASfast @22:26:09.26 AS.seek 0ml @22:26:36.60 delay 3 seconds @22:26:39.62 AFV.select :carrierSolution @22:26:39.73 delay 2 seconds @22:26:41.76 AS.reconfigure ASconfig @22:26:41.82 AS.seek 1ml @22:27:39.61 delay 1 second @22:27:40.62 AFV.select :holdingCoil @22:27:40.73 delay 1 second @22:27:41.76 AS.reconfigure ASfast @22:27:41.81 AS.seek 0ml @22:28:09.16 delay 3 seconds @22:28:12.18 AFV.select :carrierSolution @22:28:12.29 delay 2 seconds @22:28:14.32 AS.reconfigure ASconfig @22:28:14.37 AS.seek 1ml @22:29:12.18 delay 1 second @22:29:13.19 AFV.select :holdingCoil @22:29:13.30 delay 1 second @22:29:14.33 AS.reconfigure ASfast @22:29:14.38 AS.seek 0ml @22:29:41.74 delay 3 seconds @22:29:44.76 AFV.select :carrierSolution @22:29:44.87 delay 2 seconds @22:29:46.90 AS.reconfigure ASconfig @22:29:46.95 AS.seek 1ml @22:30:45.33 delay 1 second @22:30:46.34 AFV.select :holdingCoil @22:30:46.45 delay 1 second @22:30:47.48 AS.reconfigure ASnorm @22:30:47.53 AS.seek 0ml @22:31:38.25 AFV.select :carrierSolution @22:31:38.36 delay 2 seconds @22:31:40.39 AS.reconfigure ASconfig @22:31:40.44 AS.seek 1ml @22:32:38.24 delay 1 second @22:32:39.26 AFV.select :holdingCoil @22:32:39.37 delay 1 second @22:32:40.39 ATV.dial :waste @22:32:41.57 AS.reconfigure ASnorm @22:32:41.63 AS.seek 0.5ml @22:33:07.17 ATV.dial :waste2 @22:33:08.41 AS.seek 0ml @22:33:33.93 AS.coast @22:33:33.98 SS.coast @22:33:34.02 CC.coast @22:33:34.06 CS.coast @22:33:34.10 PC.coast @22:33:34.14 PS.coast @22:33:34.18 Forearm.coast @22:33:34.22 Elbow.coast @22:33:34.64 Gate.power :core,:OFF @22:33:34.65 Gate.power :raw,:OFF @22:33:34.66 Shutdown @22:33:34.68 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @08:17:12.37CEST21-Oct-18 Session Begun @08:17:15.78 -> exit @08:17:15.79 Session Ended @08:17:23.94 Session Begun @08:17:33.69 -> ESP.wakeup @08:17:33.75 Powering core bus @08:17:38.49 Gate.power :raw,:ON @08:17:39.24 SPE.coast @08:17:39.33 SPE.configure SPEconfig @08:17:39.45 AS.configure ASconfig @08:17:39.58 SS.configure SSconfig @08:17:39.71 CC.configure CCconfig @08:17:39.83 CS.configure CSconfig @08:17:39.96 PC.configure PCconfig @08:17:40.09 PS.configure PSconfig @08:17:40.24 Forearm.configure LooseForearm @08:17:40.37 Elbow.configure LooseElbow @08:17:40.51 SC.configure SCloose @08:17:40.64 SE.configure SEconfig @08:17:40.71 CH.coast @08:17:40.79 CH.configure CHconfig @08:17:40.85 PH.coast @08:17:40.94 PH.configure PHconfig @08:17:41.00 PCR.off @08:17:41.05 PCR.pidtemp= 50,0.1,0,300,25,25 @08:17:41.09 PCR.caltemp= 2.7073,0.0373,0 @08:17:41.14 All dwarf controllers running firmware version 3.75 @08:17:41.28 Valve.reset @08:17:41.47 PCR.off @08:17:41.52 PCR.pidtemp= 50,0.1,0,300,25,25 @08:17:41.57 PCR.caltemp= 2.7073,0.0373,0 @08:17:41.68 Intake.select :close @08:17:41.77 Exhaust.select :close @08:17:41.82 CTV.dial :air @08:17:41.98 PSV.dial :air @08:17:42.02 CSV.dial :waste1 @08:17:42.23 SSV.dial :spit,avoiding: :exhaust @08:17:42.25 Forearm.reconfigure LooseForearm @08:17:42.39 Forearm.home.seek :retract @08:17:42.61 PS.reconfigure PSconfig @08:17:42.69 PS.home.seek 0ml @08:17:43.19 Hand.close @08:17:43.23 Elbow.jog 1500 @08:17:43.39 CS.reconfigure CSfast @08:17:43.42 SS.reconfigure SSconfig @08:17:43.52 CS.home.seek 0ml @08:17:43.57 SS.home.seek 0ml @08:17:43.88 Elbow.reconfigure LooseElbow @08:17:43.95 Elbow.home.seek :clear @08:17:47.38 SE.reconfigure SEconfig @08:17:47.48 SE.home.seek :down @08:17:48.23 SC.reconfigure SCsloppy @08:17:48.30 SC.home.seek :initial @08:17:51.85 PS.coast @08:17:54.08 Forearm.seek :retract @08:17:54.25 Elbow.seek :hammer @08:17:55.62 Forearm.reconfigure TightForearm @08:17:55.70 Forearm.seek 5 + 350 counts @08:17:56.77 Forearm.seek :retract @08:17:58.00 PC.reconfigure PCconfig @08:17:58.02 CC.reconfigure CCconfig @08:17:58.13 PC.home.seek :open @08:17:58.22 CC.home.seek :open @08:19:26.02 -> ATV @08:19:42.79 -> MFB.ready? @08:19:48.50 -> MFB.ready! @08:19:48.63 Readying the MFB @08:19:48.65 Homing MFB Syringe @08:19:48.67 AFV.select :holdingCoil @08:19:48.86 ATV.dial :air2 @08:19:49.42 AS.reconfigure ASconfig @08:19:49.50 AS.home.seek :home @08:19:55.04 AFV.select :carrierSolution @08:19:55.18 delay 2 seconds @08:19:57.21 AS.seek 1ml @08:20:50.17 delay 1 second @08:20:51.18 AFV.select :holdingCoil @08:20:51.31 delay 1 second @08:20:52.34 ATV.dial :waste @08:20:53.36 AS.reconfigure ASnorm @08:20:53.43 AS.home.seek :empty @08:21:46.02 ATV.dial :waste @08:21:46.04 ARV.dial between carrier and air1 @08:21:46.72 ASV.dial :waste1 @08:23:23.42 -> ATV.ARV @08:23:23.44 ATV.dial ARV @08:23:35.46 -> ARV.air3 @08:23:35.48 ARV.dial :air3 @08:24:16.09 -> ATV @08:24:21.43 -> ARV @08:24:34.40 -> AS.pull 0.2, ASslow1 @08:24:34.51 AS.reconfigure ASslow1 @08:24:34.59 AS.seek 0.199ml @08:25:27.56 -> ARV.PP4 @08:25:27.58 ARV.dial :PP4 @08:25:30.79 -> ATV @08:25:57.55 -> AS.pull 0.02, ASslow1 @08:25:57.65 AS.seek 0.219ml @08:26:09.52 -> ARV.air3 @08:26:09.54 ARV.dial :air3 @08:26:33.29 -> AS.pull 0.2, ASslow1 @08:26:33.40 AS.seek 0.419ml @08:27:24.22 -> ATV.waste @08:27:24.24 ATV.dial :waste @08:27:34.51 -> AS.empty @08:27:34.53 AS.reconfigure ASconfig @08:27:34.62 AS.seek :empty @08:28:12.75 -> pcrDecon @08:28:12.85 Cleaning PCR @08:28:12.86 AFV.select :carrierSolution @08:28:13.00 delay 2 seconds @08:28:15.03 AS.seek 0.5ml @08:28:44.13 delay 1 second @08:28:45.14 AFV.select :holdingCoil @08:28:45.32 ASV.dial :waste1 @08:28:45.35 ATV.dial :air3 @08:28:46.24 AS.reconfigure ASslow1 @08:28:46.31 AS.seek 0.51ml @08:28:48.61 delay 2 seconds @08:28:50.63 ATV.dial :bleach @08:28:51.10 AS.seek 0.81ml @08:29:13.33 Session Begun @08:29:51.69 delay 3 seconds @08:29:54.71 ATV.dial :air3 @08:29:55.27 delay 3 seconds @08:29:58.36 AS.seek 0.835ml @08:30:03.68 delay 3 seconds @08:30:06.71 ATV.dial :bleach @08:30:07.35 AS.seek 0.82ml @08:30:10.75 delay 3 seconds @08:30:13.77 ARV.dial :waste @08:30:14.80 delay 3 seconds @08:30:17.82 ATV.dial :ARV @08:30:18.70 AS.reconfigure ASconfig @08:30:18.79 AS.seek 0.47ml @08:30:39.21 AFV.select :carrierSolution @08:30:39.37 delay 2 seconds @08:30:41.40 AS.seek 1ml @08:31:12.19 delay 1 second @08:31:13.21 AFV.select :holdingCoil @08:31:13.35 delay 1 second @08:31:14.38 AS.reconfigure ASfast @08:31:14.46 AS.seek 0ml @08:31:41.82 AFV.select :carrierSolution @08:31:41.98 delay 2 seconds @08:31:44.01 AS.reconfigure ASconfig @08:31:44.09 AS.seek 1ml @08:32:41.91 delay 1 second @08:32:42.92 AFV.select :holdingCoil @08:32:43.06 delay 1 second @08:32:44.09 AS.reconfigure ASfast @08:32:44.17 AS.seek 0ml @08:33:11.54 AFV.select :carrierSolution @08:33:11.70 delay 2 seconds @08:33:13.73 AS.reconfigure ASconfig @08:33:13.82 AS.seek 1ml @08:34:11.63 delay 1 second @08:34:12.64 AFV.select :holdingCoil @08:34:12.78 delay 1 second @08:34:13.81 AS.reconfigure ASfast @08:34:13.89 AS.seek 0ml @08:34:41.26 ARV.dial between carrier and air1 @08:34:41.80 ATV.dial :air3 @08:34:43.03 AS.reconfigure ASslow1 @08:34:43.12 AS.seek 0.01ml @08:34:45.49 delay 2 seconds @08:34:47.50 ATV.dial :bleach @08:34:48.14 AS.seek 0.81ml @08:37:29.30 delay 3 seconds @08:37:32.32 ATV.dial :air3 @08:37:32.88 delay 3 seconds @08:37:35.97 AS.seek 0.835ml @08:37:41.33 delay 3 seconds @08:37:44.35 ATV.dial :bleach @08:37:45.00 AS.seek 0.82ml @08:37:48.33 delay 3 seconds @08:37:51.35 ATV.dial PCR @08:37:51.77 AS.reconfigure ASconfig @08:37:51.85 AS.seek :empty @08:38:39.32 PCR.program :bleachProfile @08:38:39.41 PCR program should complete in < 2:45 @08:38:39.43 PCR.run 10:00 @08:42:11.67 PCR.off @08:42:11.73 AFV.select :carrierSolution @08:42:11.87 delay 2 seconds @08:42:13.90 AS.seek 1ml @08:43:11.77 delay 1 second @08:43:12.79 AFV.select :holdingCoil @08:43:12.94 delay 1 second @08:43:13.97 AS.reconfigure ASfast @08:43:14.06 AS.seek 0ml @08:43:41.44 delay 3 seconds @08:43:44.46 AFV.select :carrierSolution @08:43:44.61 delay 2 seconds @08:43:46.64 AS.reconfigure ASconfig @08:43:46.74 AS.seek 1ml @08:44:44.55 delay 1 second @08:44:45.57 AFV.select :holdingCoil @08:44:45.71 delay 1 second @08:44:46.74 AS.reconfigure ASfast @08:44:46.82 AS.seek 0ml @08:45:14.20 delay 3 seconds @08:45:17.22 AFV.select :carrierSolution @08:45:17.37 delay 2 seconds @08:45:19.40 AS.reconfigure ASconfig @08:45:19.52 AS.seek 1ml @08:46:17.35 delay 1 second @08:46:18.36 AFV.select :holdingCoil @08:46:18.50 delay 1 second @08:46:19.53 AS.reconfigure ASfast @08:46:19.61 AS.seek 0ml @08:46:47.07 delay 3 seconds @08:46:50.09 AFV.select :carrierSolution @08:46:50.24 delay 2 seconds @08:46:52.27 AS.reconfigure ASconfig @08:46:52.37 AS.seek 1ml @08:47:50.24 delay 1 second @08:47:51.25 AFV.select :holdingCoil @08:47:51.39 delay 1 second @08:47:52.42 AS.reconfigure ASfast @08:47:52.50 AS.seek 0ml @08:48:19.87 delay 3 seconds @08:48:22.89 AFV.select :carrierSolution @08:48:23.03 delay 2 seconds @08:48:25.06 AS.reconfigure ASconfig @08:48:25.14 AS.seek 1ml @08:49:22.98 delay 1 second @08:49:23.99 AFV.select :holdingCoil @08:49:24.13 delay 1 second @08:49:25.16 AS.reconfigure ASnorm @08:49:25.25 AS.seek 0ml @08:50:40.51 -> exit @08:50:40.53 Session Ended @08:51:10.47 -> ESP.shutdown @08:51:10.51 AS.coast @08:51:10.54 Asleep / Shutdown @08:51:10.61 SS.coast @08:51:10.67 CC.coast @08:51:10.73 CS.coast @08:51:10.79 PC.coast @08:51:10.85 Forearm.coast @08:51:10.91 Elbow.coast @08:51:10.96 SC.coast @08:51:11.02 SE.coast @08:51:11.44 Gate.power :core,:OFF @08:51:11.46 Gate.power :raw,:OFF @08:51:11.48 Shutdown @08:52:14.01 Session Ended @08:52:57.20 Session Begun @08:53:07.28 -> showlog 50 @08:53:41.99 -> can @08:54:15.45 -> exit @08:54:15.47 Session Ended @14:00:26.79
Powering core bus @14:00:31.49 Gate.power :raw,:ON @14:00:32.17 SPE.coast @14:00:32.23 SPE.configure SPEconfig @14:00:32.32 AS.configure ASconfig @14:00:32.43 SS.configure SSconfig @14:00:32.53 CC.configure CCconfig @14:00:32.63 CS.configure CSconfig @14:00:32.73 PC.configure PCconfig @14:00:32.83 PS.configure PSconfig @14:00:32.95 Forearm.configure LooseForearm @14:00:33.05 Elbow.configure LooseElbow @14:00:33.17 SC.configure SCloose @14:00:33.26 SE.configure SEconfig @14:00:33.31 CH.coast @14:00:33.37 CH.configure CHconfig @14:00:33.40 PH.coast @14:00:33.46 PH.configure PHconfig @14:00:33.50 PCR.off @14:00:33.54 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:33.56 PCR.caltemp= 2.7073,0.0373,0 @14:00:33.59 All dwarf controllers running firmware version 3.75 @14:00:33.71 Valve.reset @14:00:33.88 PCR.off @14:00:33.91 PCR.pidtemp= 50,0.1,0,300,25,25 @14:00:33.94 PCR.caltemp= 2.7073,0.0373,0 @14:00:34.18 Intake.select :close @14:00:34.20 Exhaust.select :close @14:00:34.21 CTV.dial :air @14:00:34.31 PSV.dial :air @14:00:34.47 CSV.dial :waste1 @14:00:35.01 PS.reconfigure PSconfig @14:00:35.06
Forearm.reconfigure LooseForearm @14:00:35.19 PS.home.seek 0ml @14:00:35.22
Forearm.home.seek :retract @14:00:35.33 SSV.dial :spit,avoiding: :exhaust @14:00:35.48 CS.reconfigure CSfast @14:00:35.51 SS.reconfigure SSconfig @14:00:35.61 CS.home.seek 0ml @14:00:35.65 SS.home.seek 0ml @14:00:36.02 Hand.close @14:00:36.05
Elbow.jog 1500 @14:00:36.62 Elbow.reconfigure LooseElbow @14:00:36.67 Elbow.home.seek :clear @14:00:41.17 SE.reconfigure SEconfig @14:00:41.22 SE.home.seek :down @14:00:41.82 SC.reconfigure SCsloppy @14:00:41.87 SC.home.seek :initial @14:00:44.32 PS.coast @14:00:45.87
Forearm.seek :retract @14:00:45.95 Elbow.seek :hammer @14:00:47.07 Forearm.reconfigure TightForearm @14:00:47.12 Forearm.seek 5 + 350 counts @14:00:48.27 Forearm.seek :retract @14:00:49.50 CC.reconfigure CCconfig @14:00:49.52 PC.reconfigure PCconfig @14:00:49.61
CC.home.seek :open @14:00:49.64 PC.home.seek :open @14:00:53.13
Phase [mbaPhase] @14:00:53.15 GoodNews.email "At 14:00:53 began in tube 4: mbaPhase\n/home/esp/esp2/mission/18tundeployv4.rb:127\n{1 => 22, 2 => 22, 3 => 4, 4 => 18}\nCan@14:00:53, 13.4C, 72% humidity, 14.3psia, 12.832V, 0.846A, 0.846A avg, 10.86W",Subject: "Started 18tundeployv4 phase mbaPhase" @14:00:53.17CEST21-Oct-18
==================> STARTED Superphase (phase #3) <=== @14:00:53.19 Starting initialPurge @14:00:53.21 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:00:53.29 Hand.open @14:00:54.20 Elbow.reconfigure TightElbow @14:00:54.25 Elbow.seek FlushPuck::Garage @14:00:56.19 Elbow.reconfigure LooseElbow @14:00:56.30 Forearm.reconfigure LooseForearm @14:00:56.35 Forearm.seek :garage @14:00:57.32 Hand.close @14:00:58.20 Forearm.seek :retract @14:00:59.23 Elbow.reconfigure TightElbow @14:00:59.28 Elbow.seek CC @14:01:02.22 Elbow.reconfigure LooseElbow @14:01:02.37 Forearm.reconfigure TightForearm @14:01:02.42 Forearm.seek CC @14:01:03.72 CC.seek :closed @14:01:07.97 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.17 @14:01:08.06 Hand.open @14:01:08.90 Forearm.reconfigure LooseForearm @14:01:08.94 Forearm.seek :retract @14:01:09.95 Flushing the Collection Valve::Manifold @14:01:10.08 CSV.dial CSR @14:01:11.57 Pulling 5ml of CSR.flush @14:01:11.58 CSR.select :flush @14:01:11.70 CS.reconfigure CSconfig @14:01:11.74 CS.seek 5ml @14:01:25.56 delay 5 seconds @14:01:30.58 CSR.select :air @14:01:30.75 CS.reconfigure CSfast @14:01:30.80 CS.seek 7.5ml @14:01:37.80 CSV.dial :waste @14:01:39.21 CS.reconfigure CSconfig @14:01:39.26 CS.seek 0ml @14:01:59.40 Purging external Intake @14:01:59.53 CSV.dial :flush @14:02:00.03 CS.reconfigure CSfast @14:02:00.08 CS.seek 10ml @14:02:25.15 delay 5 seconds @14:02:30.18 CSV.dial CBV @14:02:30.22
CBV.dial CSV @14:02:31.03 CTV.dial :intake @14:02:31.84 Intake.select :open @14:02:31.97 CS.seek 0ml @14:02:58.39 Intake.select :close @14:02:58.51 CTV.dial :air @14:02:59.32 Flushing upper manifold @14:02:59.45 CSV.dial :flush @14:03:00.25 CS.seek 5ml @14:03:13.81 delay 5 seconds @14:03:18.83 CSV.dial :air @14:03:19.52 CS.seek 7ml @14:03:25.40 CSV.dial CBV @14:03:25.42
CBV.dial CSV @14:03:26.67 CTV.dial :air @14:03:26.69 CS.seek 0ml @14:03:44.81 delay 7 seconds @14:03:51.84 CS.seek 3ml @14:04:00.11 CSV.dial :waste @14:04:00.60 CS.seek 0ml @14:04:08.84 CSV.dial :flush @14:04:09.32 CS.seek 3ml @14:04:17.62 delay 5 seconds @14:04:22.64 CSV.dial :air @14:04:23.21 CS.seek 5ml @14:04:28.96 CSV.dial :air @14:04:28.99 CS.seek 0ml @14:04:41.93 delay 7 seconds @14:04:48.96 CS.seek 3ml @14:04:57.18 CSV.dial :waste @14:04:58.00 CS.seek 0ml @14:05:06.19 Garaging FlushPuck in Collection Clamp @14:05:06.27 Elbow.reconfigure TightElbow @14:05:06.32 Elbow.seek CC @14:05:06.57 Elbow.reconfigure LooseElbow @14:05:06.68 Forearm.seek CC @14:05:07.64 Hand.close @14:05:08.45 CC.seek :open @14:05:12.81 Forearm.seek :retract @14:05:13.80 Elbow.reconfigure TightElbow @14:05:13.85 Elbow.seek :garage @14:05:17.04 Elbow.reconfigure LooseElbow @14:05:17.14 Forearm.reconfigure TightForearm @14:05:17.19 Forearm.seek :garage @14:05:18.33 Hand.open @14:05:19.15 Forearm.reconfigure LooseForearm @14:05:19.19 Forearm.seek :retract @14:05:20.29 Starting mfb @14:05:20.30 pcrBAC sampling at most 300ml, wcr at most 1500ml @14:05:20.35 BACfans.email "Started mfb in tube 4 at 14:05:20CEST21-Oct-18\n{1 => 22, 2 => 22, 3 => 4, 4 => 18}\nCan@14:04:37, 13.4C, 72% humidity, 14.3psia, 12.842V, 0.714A, 0.714A avg, 9.17W",Subject: "pcrBAC sampling at most 300ml, wcr at most 1500ml" @14:05:20.41
Loading sh1 puck from tube 4 into Collection Clamp @14:05:20.54 Readying the MFB @14:05:20.60 Homing MFB Syringe @14:05:20.61 AFV.select :holdingCoil @14:05:20.84 ATV.dial :air2 @14:05:20.88
SC.reconfigure SCtight @14:05:20.98 SC.seek 4 @14:05:22.32 AS.reconfigure ASconfig @14:05:22.37 AS.home.seek :home @14:05:24.60
SC.reconfigure SCloose @14:05:24.85 Elbow.reconfigure TightElbow @14:05:24.90 Elbow.seek 4 @14:05:27.00 Forearm.reconfigure TightForearm @14:05:27.06 Forearm.seek 4 + 700 counts @14:05:28.29 AFV.select :carrierSolution @14:05:28.45
SE.reconfigure SEpick @14:05:28.51 delay 2 seconds @14:05:28.53
SE.seek beamBreak @14:05:30.20 SE.configure SEconfig @14:05:30.45 Elbow.seek 4 @14:05:30.64 AS.seek 1ml @14:05:30.74
Forearm.seek 4 + 300 counts @14:05:31.11 Forearm.reconfigure LooseForearm @14:05:31.16 Hand.close @14:05:31.98 SE.seek :down @14:05:32.23
Forearm.seek :retract @14:05:33.20 Elbow.seek CC @14:05:33.28 SE.coast @14:05:34.79
Elbow.reconfigure LooseElbow @14:05:34.89 Forearm.reconfigure TightForearm @14:05:34.94 Forearm.seek CC @14:05:36.27 CC.seek :closed @14:05:40.53 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.23 @14:05:40.54 Hand.open @14:05:41.40 Forearm.reconfigure LooseForearm @14:05:41.44 Forearm.seek :retract @14:05:42.41 SC.coast @14:05:42.46 collectSample 300ml w/bubble point of 55psi @14:05:42.47 Priming 7ml @14:05:42.48 Intake.select :close @14:05:42.49 Exhaust.select :close @14:05:42.51 SS.hold @14:05:42.73 Calibrating pressure and temperature sensors @14:05:42.75 CTV.dial :air @14:05:42.76 SSV.dial :spit,avoiding: :exhaust @14:05:42.79 SS.reconfigure SSfast @14:05:42.84 SS.seek 5ml @14:05:45.51 SSV.dial CBV @14:05:45.54
CBV.dial SSV @14:05:47.01 delay 3.8 seconds @14:05:50.96 SSV.dial :spit @14:05:52.40 SS.seek 0ml @14:05:55.25 SSV.dial CBV @14:05:56.67 CTV.dial :intake @14:05:57.50 SSV.dial CBV @14:05:57.53
CBV.dial SSV @14:05:57.55 Allowing 40s for 7.0ml of remaining 7.0ml @14:05:57.56 Intake.select :open @14:05:59.73 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:05:59.75 SS.reconfigure SSinhale.with maxSpeed: 150 @14:05:59.80 SS.seek 7ml @14:06:03.57 Vacuum too high -- waiting up to 40s @14:06:04.94 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:06:04.96 SS.reconfigure SSinhale.with maxSpeed: 135 @14:06:05.01 SS.seek 7ml @14:06:07.88 Vacuum too high -- waiting up to 40s @14:06:09.23 Intake @32.8psia, vacuum from 20.0 to 24.0psi @14:06:09.25 SS.reconfigure SSinhale.with maxSpeed: 122 @14:06:09.30 SS.seek 7ml @14:06:10.49 Stroke completed -- waiting for pressure to equalize ... @14:06:12.47 Intake.select :close @14:06:12.63 SSV.dial :exhaust @14:06:13.44 Exhaust.select :open @14:06:13.56 SS.reconfigure SSexhale @14:06:13.62 SS.seek 0ml @14:06:17.41 Exhaust.select :close @14:06:17.53 Sampling 300.0ml @14:06:17.54 Intake.select :close @14:06:17.55 Exhaust.select :close @14:06:17.57 SS.reconfigure SSconfig @14:06:17.62 SS.hold @14:06:17.84 CTV.dial :intake @14:06:17.85 SSV.dial CBV @14:06:17.88
CBV.dial SSV @14:06:18.74 Allowing 130s for 25.0ml of remaining 300.0ml @14:06:18.75 Intake.select :open @14:06:20.94 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:06:20.95 SS.reconfigure SSinhale.with maxSpeed: 122 @14:06:21.01 SS.seek 25ml @14:06:23.57 delay 1 second @14:06:24.58 AFV.select :holdingCoil @14:06:24.69 delay 1 second @14:06:24.85
Vacuum too high -- waiting up to 40s @14:06:25.71 ATV.dial :waste @14:06:26.18
Intake @33.5psia, vacuum from 20.0 to 24.0psi @14:06:26.20 SS.reconfigure SSinhale.with maxSpeed: 110 @14:06:26.25 SS.seek 25ml @14:06:26.73 AS.reconfigure ASnorm @14:06:26.78 AS.home.seek :empty @14:06:29.07
Vacuum too high -- waiting up to 40s @14:06:30.41 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:06:30.43 SS.reconfigure SSinhale.with maxSpeed: 99 @14:06:30.48 SS.seek 25ml @14:06:33.32 Vacuum too high -- waiting up to 40s @14:06:34.67 Intake @32.8psia, vacuum from 20.0 to 24.0psi @14:06:34.69 SS.reconfigure SSinhale.with maxSpeed: 89 @14:06:34.74 SS.seek 25ml @14:06:38.35 Vacuum too high -- waiting up to 40s @14:06:39.70 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:06:40.25 SS.reconfigure SSinhale.with maxSpeed: 80 @14:06:40.31 SS.seek 25ml @14:06:44.55 Vacuum too high -- waiting up to 40s @14:06:45.65 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:06:45.66 SS.reconfigure SSinhale.with maxSpeed: 72 @14:06:45.71 SS.seek 25ml @14:06:49.45 Vacuum too high -- waiting up to 40s @14:06:50.57 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:06:50.59 SS.reconfigure SSinhale.with maxSpeed: 65 @14:06:50.64 SS.seek 25ml @14:06:55.73 Vacuum too high -- waiting up to 40s @14:06:56.83 Intake @33.0psia, vacuum from 20.0 to 24.0psi @14:06:56.85 SS.reconfigure SSinhale.with maxSpeed: 59 @14:06:56.90 SS.seek 25ml @14:06:59.28 Stroke completed -- waiting for pressure to equalize ... @14:07:01.27 Intake.select :close @14:07:01.43 SSV.dial :exhaust @14:07:02.26 Exhaust.select :open @14:07:02.37 SS.reconfigure SSexhale @14:07:02.42 SS.seek 0ml @14:07:11.60 Exhaust.select :close @14:07:11.72 SSV.dial CBV @14:07:11.75
CBV.dial SSV @14:07:12.61 Allowing 130s for 25.0ml of remaining 275.0ml @14:07:12.62 Intake.select :open @14:07:14.79 Intake @33.2psia, vacuum from 20.0 to 24.0psi @14:07:14.81 SS.reconfigure SSinhale.with maxSpeed: 59 @14:07:14.86 SS.seek 25ml @14:07:19.36 ATV.dial :waste @14:07:19.38 ARV.dial between carrier and air1 @14:07:19.46 ASV.dial :waste1 @14:07:25.67
Vacuum too high -- waiting up to 40s @14:07:26.74 Intake @32.9psia, vacuum from 20.0 to 24.0psi @14:07:26.76 SS.reconfigure SSinhale.with maxSpeed: 53 @14:07:26.81 SS.seek 25ml @14:07:49.17 Stroke completed -- waiting for pressure to equalize ... @14:07:51.74 Intake.select :close @14:07:51.89 SSV.dial :exhaust @14:07:52.73 Exhaust.select :open @14:07:52.84 SS.reconfigure SSexhale @14:07:52.90 SS.seek 0ml @14:08:01.86 Exhaust.select :close @14:08:01.98 SSV.dial CBV @14:08:02.01
CBV.dial SSV @14:08:02.86 Allowing 130s for 25.0ml of remaining 250.0ml @14:08:02.87 Intake.select :open @14:08:05.04 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:08:05.06 SS.reconfigure SSinhale.with maxSpeed: 53 @14:08:05.11 SS.seek 25ml @14:08:32.26 Vacuum too high -- waiting up to 40s @14:08:33.10 Intake @33.1psia, vacuum from 20.0 to 24.0psi @14:08:33.72 SS.reconfigure SSinhale.with maxSpeed: 48 @14:08:33.78 SS.seek 25ml @14:08:42.14 Stroke completed -- waiting for pressure to equalize ... @14:08:44.11 Intake.select :close @14:08:44.27 SSV.dial :exhaust @14:08:45.11 Exhaust.select :open @14:08:45.22 SS.reconfigure SSexhale @14:08:45.27 SS.seek 0ml @14:08:54.02 Exhaust.select :close @14:08:54.14 SSV.dial CBV @14:08:54.17
CBV.dial SSV @14:08:55.00 Allowing 130s for 25.0ml of remaining 225.0ml @14:08:55.01 Intake.select :open @14:08:57.18 Intake @34.1psia, vacuum from 20.0 to 24.0psi @14:08:57.20 SS.reconfigure SSinhale.with maxSpeed: 48 @14:08:57.25 SS.seek 25ml @14:09:34.71 Stroke completed -- waiting for pressure to equalize ... @14:09:37.31 Intake.select :close @14:09:37.47 SSV.dial :exhaust @14:09:38.31 Exhaust.select :open @14:09:38.42 SS.reconfigure SSexhale @14:09:38.47 SS.seek 0ml @14:09:47.39 Exhaust.select :close @14:09:47.51 SSV.dial CBV @14:09:47.54
CBV.dial SSV @14:09:48.39 Allowing 130s for 25.0ml of remaining 200.0ml @14:09:48.40 Intake.select :open @14:09:50.57 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:09:50.59 SS.reconfigure SSinhale.with maxSpeed: 48 @14:09:50.64 SS.seek 25ml @14:10:28.05 Stroke completed -- waiting for pressure to equalize ... @14:10:30.70 Intake.select :close @14:10:30.86 SSV.dial :exhaust @14:10:31.69 Exhaust.select :open @14:10:31.80 SS.reconfigure SSexhale @14:10:31.86 SS.seek 0ml @14:10:40.99 Exhaust.select :close @14:10:41.11 SSV.dial CBV @14:10:41.14
CBV.dial SSV @14:10:41.98 Allowing 130s for 25.0ml of remaining 175.0ml @14:10:41.99 Intake.select :open @14:10:44.16 Intake @33.9psia, vacuum from 20.0 to 24.0psi @14:10:44.18 SS.reconfigure SSinhale.with maxSpeed: 48 @14:10:44.22 SS.seek 25ml @14:11:21.53 Stroke completed -- waiting for pressure to equalize ... @14:11:24.10 Intake.select :close @14:11:24.26 SSV.dial :exhaust @14:11:25.08 Exhaust.select :open @14:11:25.20 SS.reconfigure SSexhale @14:11:25.25 SS.seek 0ml @14:11:34.39 Exhaust.select :close @14:11:34.51 SSV.dial CBV @14:11:34.54
CBV.dial SSV @14:11:35.39 Allowing 130s for 25.0ml of remaining 150.0ml @14:11:35.40 Intake.select :open @14:11:37.57 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:11:37.59 SS.reconfigure SSinhale.with maxSpeed: 48 @14:11:37.64 SS.seek 25ml @14:12:14.72 Stroke completed -- waiting for pressure to equalize ... @14:12:17.30 Intake.select :close @14:12:17.96 SSV.dial :exhaust @14:12:18.81 Exhaust.select :open @14:12:18.92 SS.reconfigure SSexhale @14:12:18.97 SS.seek 0ml @14:12:28.22 Exhaust.select :close @14:12:28.34 SSV.dial CBV @14:12:28.37
CBV.dial SSV @14:12:29.22 Allowing 130s for 25.0ml of remaining 125.0ml @14:12:29.23 Intake.select :open @14:12:31.41 Intake @33.8psia, vacuum from 20.0 to 24.0psi @14:12:31.42 SS.reconfigure SSinhale.with maxSpeed: 48 @14:12:31.47 SS.seek 25ml @14:13:08.97 Stroke completed -- waiting for pressure to equalize ... @14:13:11.56 Intake.select :close @14:13:11.73 SSV.dial :exhaust @14:13:12.53 Exhaust.select :open @14:13:12.65 SS.reconfigure SSexhale @14:13:12.70 SS.seek 0ml @14:13:21.80 Exhaust.select :close @14:13:21.92 SSV.dial CBV @14:13:21.95
CBV.dial SSV @14:13:22.79 Allowing 130s for 25.0ml of remaining 100.0ml @14:13:22.80 Intake.select :open @14:13:24.98 Intake @33.4psia, vacuum from 20.0 to 24.0psi @14:13:25.00 SS.reconfigure SSinhale.with maxSpeed: 48 @14:13:25.05 SS.seek 25ml @14:14:02.32 Stroke completed -- waiting for pressure to equalize ... @14:14:04.90 Intake.select :close @14:14:05.06 SSV.dial :exhaust @14:14:05.88 Exhaust.select :open @14:14:06.00 SS.reconfigure SSexhale @14:14:06.06 SS.seek 0ml @14:14:15.08 Exhaust.select :close @14:14:15.20 SSV.dial CBV @14:14:15.23
CBV.dial SSV @14:14:16.06 Allowing 130s for 25.0ml of remaining 75.0ml @14:14:16.07 Intake.select :open @14:14:18.24 Intake @34.2psia, vacuum from 20.0 to 24.0psi @14:14:18.26 SS.reconfigure SSinhale.with maxSpeed: 48 @14:14:18.31 SS.seek 25ml @14:14:55.69 Stroke completed -- waiting for pressure to equalize ... @14:14:58.26 Intake.select :close @14:14:58.42 SSV.dial :exhaust @14:14:59.25 Exhaust.select :open @14:14:59.37 SS.reconfigure SSexhale @14:14:59.43 SS.seek 0ml @14:15:08.22 Exhaust.select :close @14:15:08.34 SSV.dial CBV @14:15:08.37
CBV.dial SSV @14:15:09.22 Allowing 130s for 25.0ml of remaining 50.0ml @14:15:09.23 Intake.select :open @14:15:11.40 Intake @34.4psia, vacuum from 20.0 to 24.0psi @14:15:11.41 SS.reconfigure SSinhale.with maxSpeed: 48 @14:15:11.46 SS.seek 25ml @14:15:49.36 Stroke completed -- waiting for pressure to equalize ... @14:15:51.93 Intake.select :close @14:15:52.08 SSV.dial :exhaust @14:15:52.89 Exhaust.select :open @14:15:53.01 SS.reconfigure SSexhale @14:15:53.06 SS.seek 0ml @14:16:02.30 Exhaust.select :close @14:16:02.42 SSV.dial CBV @14:16:02.45
CBV.dial SSV @14:16:03.29 Allowing 130s for 25.0ml of remaining 25.0ml @14:16:03.30 Intake.select :open @14:16:05.98 Intake @34.0psia, vacuum from 20.0 to 24.0psi @14:16:06.01 SS.reconfigure SSinhale.with maxSpeed: 48 @14:16:06.06 SS.seek 24.99ml @14:16:43.13 Stroke completed -- waiting for pressure to equalize ... @14:16:45.73 Intake.select :close @14:16:45.89 SSV.dial :exhaust @14:16:46.72 Exhaust.select :open @14:16:46.83 SS.reconfigure SSexhale @14:16:46.88 SS.seek 0ml @14:16:56.16 Exhaust.select :close @14:16:56.28 Sampled 300.0ml @14:16:56.30 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @14:16:56.37 CSV.dial :waste @14:16:56.46 CS.seek 0ml @14:16:56.54 SSV.dial CBV,avoiding: :exhaust @14:16:56.57 CSV.dial :air @14:16:56.63
CBV.dial SSV @14:16:57.46 CS.seek 10ml @14:16:58.65
CTV.dial :air @14:16:59.48 delay 15 seconds @14:17:14.51 SSV.dial :spit,avoiding: :exhaust @14:17:15.95 CTV.dial CSV,avoiding: :intake @14:17:16.01
SS.seek 0ml @14:17:16.14 SSV.dial CBV,avoiding: :exhaust @14:17:22.73 CBV.dial between SSV and waste2 @14:17:23.14 CTV.dial CSV,avoiding: :air @14:17:23.17
CSV.dial CTV @14:17:23.65 CS.reconfigure CSpressurize @14:17:23.70 CS.seek 0ml @14:17:49.82 BubblePoint: Intake @69.5psia, Outlet @14.5psia @14:17:49.83 CBV.dial SSV @14:17:50.18 SS.reconfigure SSconfig @14:17:50.23 SS.seek 10ml @14:17:55.89 delay 15 seconds @14:18:11.03 Evacuated: Intake @35.7psia, Outlet @1.0psia @14:18:11.04 SSV.dial :spit,avoiding: :exhaust @14:18:12.50 SS.reconfigure SSexhale @14:18:12.55 SS.seek 0ml @14:18:17.51 SS.reconfigure SSfast @14:18:17.57 SS.seek 5ml @14:18:20.31 SSV.dial CBV,avoiding: :exhaust @14:18:20.34
CBV.dial SSV @14:18:21.76 CTV.dial :air,avoiding: :intake @14:18:23.20 CSV.dial CTV @14:18:23.25
SS.seek 10ml @14:18:24.24 CSV.dial :air @14:18:24.74 CS.reconfigure CSfast @14:18:24.79 CS.seek 0ml @14:18:25.31 CS.coast @14:18:25.97
SSV.dial :spit,avoiding: :exhaust @14:18:27.40 SS.reconfigure SSexhale @14:18:27.45 SS.seek 0ml @14:18:32.51 SS.reconfigure SSfast @14:18:32.56 SS.seek 5ml @14:18:35.31 SSV.dial CBV,avoiding: :exhaust @14:18:35.34
CBV.dial SSV @14:18:37.80 SSV.dial :spit,avoiding: :exhaust @14:18:39.26 SS.reconfigure SSconfig @14:18:39.31 SS.seek 0ml @14:18:42.66 SS.coast @14:18:43.22 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @14:18:43.23 Intake.select :close @14:18:43.32 CSV.dial :waste @14:18:43.46 SSV.dial CBV,avoiding: :exhaust @14:18:43.49
CBV.dial SSV @14:18:44.18 CS.seek 0ml @14:18:44.25 CSV.dial :air @14:18:44.93
CTV.dial :air @14:18:44.94 delay 15 seconds @14:18:45.04 CS.seek 10ml @14:18:59.97
SSV.dial :spit,avoiding: :exhaust @14:19:01.40 CTV.dial CSV,avoiding: :intake @14:19:01.44
SS.reconfigure SSexhale @14:19:01.55 SS.seek 0ml @14:19:01.81 SSV.dial CBV,avoiding: :exhaust @14:19:10.17 CBV.dial between SSV and waste2 @14:19:10.60 CSV.dial between air and CTV @14:19:10.93 CS.seek 8.229ml @14:19:16.20 CTV.dial CSV,avoiding: :air @14:19:16.23
CSV.dial CTV @14:19:16.56 CS.reconfigure CSpressurize @14:19:16.61 CS.seek 0ml @14:19:38.19 CSV.dial :air @14:19:38.70 CS.reconfigure CSfast @14:19:38.75 CS.seek 10ml @14:20:03.76 CSV.dial between air and CTV @14:20:04.12 CS.seek 3.141ml @14:20:21.57 CTV.dial CSV,avoiding: :air @14:20:21.60
CSV.dial CTV @14:20:21.93 CS.reconfigure CSpressurize @14:20:21.98 CS.seek 0ml @14:20:30.26 BubblePoint: Intake @68.9psia, Outlet @13.8psia @14:20:30.27 CBV.dial SSV @14:20:30.62 SS.reconfigure SSconfig @14:20:30.67 SS.seek 10ml @14:20:35.94 delay 15 seconds @14:20:51.09 Evacuated: Intake @38.6psia, Outlet @15.8psia @14:20:51.10 SSV.dial :spit,avoiding: :exhaust @14:20:52.53 SS.reconfigure SSexhale @14:20:52.58 SS.seek 0ml @14:20:57.51 SS.reconfigure SSfast @14:20:57.56 SS.seek 5ml @14:21:00.45 SSV.dial CBV,avoiding: :exhaust @14:21:00.48
CBV.dial SSV @14:21:01.93 CTV.dial :air,avoiding: :intake @14:21:03.37 CSV.dial CTV @14:21:03.42
SS.seek 10ml @14:21:04.41 CSV.dial :air @14:21:04.90 CS.reconfigure CSfast @14:21:04.95 CS.seek 0ml @14:21:06.04
SSV.dial :spit,avoiding: :exhaust @14:21:06.22 CS.coast @14:21:07.47
SS.reconfigure SSexhale @14:21:07.52 SS.seek 0ml @14:21:12.51 SS.reconfigure SSfast @14:21:12.56 SS.seek 5ml @14:21:15.45 SSV.dial CBV,avoiding: :exhaust @14:21:15.48
CBV.dial SSV @14:21:18.39 SSV.dial :spit,avoiding: :exhaust @14:21:19.83 SS.reconfigure SSconfig @14:21:19.88 SS.seek 0ml @14:21:22.90 SS.coast @14:21:22.94 Lysing: Adding 1.5ml lysis @14:21:22.96 CBV.dial between CSV and waste1,avoiding: :DBV @14:21:24.59 CTV.dial CSV @14:21:26.03 CSV.dial CSR @14:21:26.89 Pulling 1ml of CSR.lysis @14:21:26.90 CSR.select :lysis @14:21:27.01 CS.reconfigure CSconfig @14:21:27.06 CS.seek 1ml @14:21:30.70 delay 5 seconds @14:21:35.72 CSR.select :air @14:21:35.91 CS.seek 6ml @14:21:49.61 delay 2 seconds @14:21:51.62 CSV.dial :waste1 @14:21:53.04 CS.reconfigure CSfast @14:21:53.09 CS.seek :empty @14:22:08.38 CSV.dial CSR @14:22:09.85 Pulling 1.5ml of CSR.lysis @14:22:09.86 CSR.select :lysis @14:22:09.98 CS.reconfigure CSconfig @14:22:10.03 CS.seek 1.5ml @14:22:14.86 delay 5 seconds @14:22:19.88 CSR.select :air @14:22:20.07 CS.seek 4.5ml @14:22:28.67 delay 2 seconds @14:22:30.68 CSV.dial CTV @14:22:31.20 CS.seek 1.275ml @14:22:40.36 Heating sh1 puck from tube 4 in Collection Clamp to 85C for 5:00 @14:22:40.38 CH.seek 85C,8:00 @14:25:41.53 CH.hold 5:00,85C @14:30:41.71 Collection Puck Heater at 85C after lysing @14:30:42.29 CS.seek 0ml @14:30:46.94 CTV.dial between spare and air,avoiding: :intake @14:30:48.07 CSV.dial :air @14:30:48.58 CS.seek 5ml @14:31:02.39 CSV.dial CTV @14:31:02.90 delay 2 seconds @14:31:04.92 CSV.dial :air @14:31:05.43 delay 2 seconds @14:31:07.44 CSV.dial CTV @14:31:07.96 delay 2 seconds @14:31:09.99 CSV.dial :air @14:31:10.49 delay 2 seconds @14:31:12.52 CS.seek 0ml @14:31:26.33 Recovering Lysate into Collection syringe @14:31:26.34 CSV.dial CBV @14:31:27.46 CBV.dial CSV @14:31:27.87 CS.seek 5ml @14:31:41.71 delay 5 seconds @14:31:46.73 CTV.dial :air @14:31:47.31 CS.seek 7ml @14:31:53.40 delay 5 seconds @14:31:58.42 CSV.dial :air2 @14:31:58.91 CS.seek 10ml @14:32:07.65 CSV.dial between air and CTV,avoiding: CBV @14:32:08.90 Lysing Complete @14:32:08.99 Storing sh1 puck in Collection Clamp to tube 3 @14:32:09.17 SC.reconfigure SCtight @14:32:09.22 SC.seek 3 @14:32:11.35 SC.reconfigure SCloose @14:32:11.57 Elbow.reconfigure TightElbow @14:32:11.62 Elbow.seek 3 @14:32:13.45 Forearm.reconfigure TightForearm @14:32:13.50 Forearm.seek 3 + 700 counts @14:32:14.51 SE.reconfigure SEpick @14:32:14.59 SE.seek beamBreak @14:32:17.45 SE.configure SEconfig @14:32:17.63 Elbow.reconfigure LimpElbow @14:32:17.68 Forearm.reconfigure LooseForearm @14:32:17.73 Forearm.seek 3 @14:32:18.07 Elbow.hold @14:32:18.14 Elbow.reconfigure TightElbow @14:32:18.24 SE.seek 3.08 Pucks below Top Plate @14:32:18.75 Forearm.force -20 @14:32:18.79 Forearm.seek 3 + 301 counts @14:32:19.12 Forearm.reconfigure TightForearm @14:32:19.17 Forearm.seek 3 + 700 counts @14:32:19.48 SE.seek 4.08 Pucks below Top Plate @14:32:20.18 Forearm.reconfigure LooseForearm @14:32:20.23 Forearm.seek :retract @14:32:21.18 Elbow.seek CC @14:32:22.91 Elbow.reconfigure LooseElbow @14:32:23.54 Forearm.seek CC @14:32:24.50 Hand.close @14:32:25.24 CC.seek :open @14:32:29.76 Forearm.seek :retract @14:32:30.73 Elbow.reconfigure TightElbow @14:32:30.78 Elbow.seek 3 @14:32:32.67 Elbow.reconfigure LimpElbow @14:32:32.72 Forearm.seek 3 @14:32:33.71 Elbow.hold @14:32:33.78 Elbow.reconfigure TightElbow @14:32:33.83 Hand.open @14:32:34.76 Forearm.force -20 @14:32:34.80 Forearm.seek 3 + 348 counts @14:32:35.15 Forearm.reconfigure TightForearm @14:32:35.20 Forearm.seek 3 + 700 counts @14:32:35.51 SE.seek :down @14:32:36.67
Forearm.reconfigure LooseForearm @14:32:36.72 Forearm.seek :retract @14:32:37.98 SE.coast @14:32:38.02
SC.coast @14:32:38.06 Tranferring lysate to PRV @14:32:38.07 CSV.dial :air @14:32:38.41 CS.seek 10ml @14:32:38.48 CSV.dial between air and CTV @14:32:38.94 AIV.dial :bypass @14:32:39.02 Beginning samplePassC @14:32:39.03 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:32:39.11 Hand.open @14:32:39.50 Elbow.seek FlushPuck::Garage @14:32:41.41 Elbow.reconfigure LooseElbow @14:32:41.52 Forearm.seek :garage @14:32:42.49 Hand.close @14:32:43.25 Forearm.seek :retract @14:32:44.24 Elbow.reconfigure TightElbow @14:32:44.29 Elbow.seek CC @14:32:47.10 Elbow.reconfigure LooseElbow @14:32:47.20 Forearm.reconfigure TightForearm @14:32:47.25 Forearm.seek CC @14:32:48.53 CC.seek :closed @14:32:53.41 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.16 @14:32:53.50 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[5C:27->20] @14:32:53.53 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[5C:27->20] @14:32:53.56 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[5C:27->20] @14:32:53.58 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[5C:27->20] @14:32:53.61 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[5C:27->20] @14:32:53.64 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[5C:27->20] @14:32:53.67 Hand.open @14:32:54.44 Forearm.reconfigure LooseForearm @14:32:54.49 Forearm.seek :retract @14:32:55.47 AWV.select :air @14:32:55.49 AIV.dial :bypass @14:32:55.50 PSV.dial PSR @14:32:56.97 Pulling 1.5ml of PSR.flush @14:32:56.98 PSR.select :flush @14:32:57.11 PS.seek 1.5ml @14:33:13.19 delay 5 seconds @14:33:18.21 PSV.dial :waste1 @14:33:18.69 PS.seek 0ml @14:33:34.74 PSV.dial PSR @14:33:35.23 PSR.select :air @14:33:35.36 PS.seek 2.5ml @14:34:01.54 PRV.dial CBV @14:34:01.59
CBV.dial PRV @14:34:02.44 PSV.dial :mixloop @14:34:03.55 CTV.dial :air,avoiding: :intake @14:34:03.56 PTV.dial :PRVmixing @14:34:03.64 PS.seek 0ml @14:34:29.78 PSV.dial :air @14:34:30.29 PS.seek 2.5ml @14:34:56.37 PSV.dial :mixloop @14:34:56.86 PS.seek 0ml @14:35:22.96 PSV.dial :air @14:35:23.44 PS.seek 2.5ml @14:35:49.59 PSV.dial :mixloop @14:35:50.07 PS.seek 0ml @14:36:16.19 Garaging FlushPuck in Collection Clamp @14:36:16.27 Elbow.reconfigure TightElbow @14:36:16.32 Elbow.seek CC @14:36:16.56 Elbow.reconfigure LooseElbow @14:36:16.67 Forearm.seek CC @14:36:17.64 Hand.close @14:36:18.37 CC.seek :open @14:36:22.78 Forearm.seek :retract @14:36:23.78 Elbow.reconfigure TightElbow @14:36:23.83 Elbow.seek :garage @14:36:26.92 Elbow.reconfigure LooseElbow @14:36:27.02 Forearm.reconfigure TightForearm @14:36:27.07 Forearm.seek :garage @14:36:28.24 Hand.open @14:36:29.07 Forearm.reconfigure LooseForearm @14:36:29.11 Forearm.seek :retract @14:36:30.15 Connecting PS to CS to transfer sample @14:36:30.16 CBV.dial AIV @14:36:30.17 AIV.dial :bypass @14:36:30.18 PSV.dial :mixloop @14:36:30.19 PTV.dial :PRVmixing @14:36:30.21 PRV.dial AIV @14:36:30.22 CTV.dial :air,avoiding: :intake @14:36:30.24 Loading lyfil puck from tube 4 into Collection Clamp @14:36:30.49 SC.reconfigure SCtight @14:36:30.54 SC.seek 4 @14:36:32.64 SC.reconfigure SCloose @14:36:33.31 Elbow.reconfigure TightElbow @14:36:33.45 Elbow.seek 4 @14:36:35.50 Forearm.reconfigure TightForearm @14:36:35.55 Forearm.seek 4 + 700 counts @14:36:36.55 SE.reconfigure SEpick @14:36:36.62 SE.seek beamBreak @14:36:38.21 SE.configure SEconfig @14:36:38.38 Elbow.seek 4 @14:36:38.59 Forearm.seek 4 + 300 counts @14:36:38.89 Forearm.reconfigure LooseForearm @14:36:38.93 Hand.close @14:36:39.64 SE.seek :down @14:36:39.87
Forearm.seek :retract @14:36:40.81 Elbow.seek CC @14:36:40.94 SE.coast @14:36:42.48
Elbow.reconfigure LooseElbow @14:36:42.59 Forearm.reconfigure TightForearm @14:36:42.64 Forearm.seek CC @14:36:43.94 CC.seek :closed @14:36:48.20 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.27 @14:36:48.22 Hand.open @14:36:49.07 Forearm.reconfigure LooseForearm @14:36:49.11 Forearm.seek :retract @14:36:50.10 SC.coast @14:36:50.14 CTV.dial CSV,avoiding: :intake @14:36:51.60 CSV.dial CTV @14:36:51.96 CS.seek 5ml @14:37:05.75 delay 1:20 @14:38:25.78 CS.seek 2.5ml @14:38:33.14 delay 40 seconds @14:39:13.18 CS.seek 0ml @14:39:20.81 delay 1:00 @14:40:20.83 PSV.dial :air @14:40:21.33 PS.seek 2.5ml @14:40:47.47 PSV.dial :mixloop @14:40:47.95 delay 3 seconds @14:40:50.97 PSV.dial :air @14:40:51.45 delay 2 seconds @14:40:53.47 PSV.dial :mixloop @14:40:53.95 delay 3 seconds @14:40:56.97 PSV.dial :waste1 @14:40:58.38 PS.seek :empty @14:41:24.59 CBV.dial :waste1 @14:41:25.11 delay 5 seconds @14:41:30.13 CBV.dial AIV @14:41:30.66 delay 10 seconds @14:41:40.68 CBV.dial :waste1 @14:41:41.20 delay 10 seconds @14:41:51.22 CBV.dial AIV @14:41:51.75 delay 10 seconds @14:42:01.78 CTV.dial CSV,avoiding: :intake @14:42:01.79 CBV.dial between AIV and waste1 @14:42:02.17 CSV.dial :air @14:42:02.66 CS.seek 10ml @14:42:29.15 CSV.dial CTV @14:42:29.66 CS.seek 0ml @14:42:56.21 CBV.dial :waste1 @14:42:56.55 delay 10 seconds @14:43:07.03 CTV.dial :air,avoiding: :intake @14:43:08.61 Storing lyfil puck in Collection Clamp to tube 3 @14:43:08.80 SC.reconfigure SCtight @14:43:08.85 SC.seek 3 @14:43:10.97 SC.reconfigure SCloose @14:43:11.20 Elbow.reconfigure TightElbow @14:43:11.25 Elbow.seek 3 @14:43:13.15 Forearm.reconfigure TightForearm @14:43:13.20 Forearm.seek 3 + 700 counts @14:43:14.23 SE.reconfigure SEpick @14:43:14.30 SE.seek beamBreak @14:43:17.02 SE.configure SEconfig @14:43:17.19 Elbow.reconfigure LimpElbow @14:43:17.25 Forearm.reconfigure LooseForearm @14:43:17.30 Forearm.seek 3 @14:43:17.58 Elbow.hold @14:43:17.65 Elbow.reconfigure TightElbow @14:43:17.76 SE.seek 4.07 Pucks below Top Plate @14:43:18.27 Forearm.force -20 @14:43:18.31 Forearm.seek 3 + 338 counts @14:43:18.62 Forearm.reconfigure TightForearm @14:43:18.67 Forearm.seek 3 + 700 counts @14:43:18.93 SE.seek 5.07 Pucks below Top Plate @14:43:19.64 Forearm.reconfigure LooseForearm @14:43:19.69 Forearm.seek :retract @14:43:20.63 Elbow.seek CC @14:43:22.40 Elbow.reconfigure LooseElbow @14:43:22.51 Forearm.seek CC @14:43:23.49 Hand.close @14:43:24.26 CC.seek :open @14:43:28.76 Forearm.seek :retract @14:43:29.77 Elbow.reconfigure TightElbow @14:43:29.82 Elbow.seek 3 @14:43:31.63 Elbow.reconfigure LimpElbow @14:43:31.69 Forearm.seek 3 @14:43:32.68 Elbow.hold @14:43:32.75 Elbow.reconfigure TightElbow @14:43:32.80 Hand.open @14:43:33.65 Forearm.force -20 @14:43:33.69 Forearm.seek 3 + 342 counts @14:43:34.01 Forearm.reconfigure TightForearm @14:43:34.06 Forearm.seek 3 + 700 counts @14:43:34.52 SE.seek :down @14:43:35.67
Forearm.reconfigure LooseForearm @14:43:35.71 Forearm.seek :retract @14:43:36.77 SE.coast @14:43:36.81
SC.coast @14:43:36.85 PSV.dial :air @14:43:38.55 PS.seek 0.499ml @14:43:44.45 delay 3 seconds @14:43:47.47 PSV.dial :mixloop @14:43:47.95 PRV.dial :air @14:43:48.45 delay 3 seconds @14:43:51.47 PRV.dial CBV @14:43:51.96 CBV.dial AIV @14:43:52.47 PRV.dial between CBV and air @14:43:52.82 PRV.dial :air @14:43:53.21 PS.reconfigure PSslow @14:43:53.26 PS.seek 0.599ml @14:43:55.72 PRV.dial :waste2 @14:43:56.52 PS.reconfigure PSconfig @14:43:56.57 PS.seek :empty @14:44:03.45 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:44:03.52 Hand.open @14:44:03.89 Elbow.seek FlushPuck::Garage @14:44:05.86 Elbow.reconfigure LooseElbow @14:44:05.97 Forearm.seek :garage @14:44:06.99 Hand.close @14:44:07.76 Forearm.seek :retract @14:44:08.82 Elbow.reconfigure TightElbow @14:44:08.87 Elbow.seek CC @14:44:11.77 Elbow.reconfigure LooseElbow @14:44:11.88 Forearm.reconfigure TightForearm @14:44:11.93 Forearm.seek CC @14:44:13.28 CC.seek :closed @14:44:17.56 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.25 @14:44:17.63 Hand.open @14:44:18.33 Forearm.reconfigure LooseForearm @14:44:18.37 Forearm.seek :retract @14:44:19.42 Clearing PML for modified lysate @14:44:19.43 CTV.dial :air,avoiding: :intake @14:44:19.44 PRV.dial :waste2 @14:44:19.45 PTV.dial :PRV,avoiding: :puck @14:44:20.26 delay 2 seconds @14:44:22.28 PTV.dial :PRVmixing,avoiding: :puck @14:44:23.09 delay 2 seconds @14:44:25.10 PSV.dial :PML @14:44:25.12 delay 2 seconds @14:44:27.14 PRV.dial :air @14:44:27.95 delay 2 seconds @14:44:30.04 PS.seek 1.499ml @14:44:46.07 delay 5 seconds @14:44:51.10 PS.seek 2.5ml @14:45:02.03 PSV.dial :air @14:45:02.51 PS.seek 0ml @14:45:28.64 mixing lysate with speDil @14:45:28.65 PSV.dial :PML @14:45:29.12 CBV.dial :PRV @14:45:29.13 PRV.dial :air @14:45:29.14 delay 2 seconds @14:45:31.16 PRV.dial :speDil @14:45:31.68 delay 2 seconds @14:45:34.30 PS.reconfigure PSslow1 @14:45:34.35 PS.seek 0.225ml @14:45:57.98 delay 5 seconds @14:46:03.01 PRV.dial CBV @14:46:03.83 delay 2 seconds @14:46:05.91 PS.seek 0.475ml @14:46:31.67 delay 5 seconds @14:46:36.70 PRV.dial :air @14:46:37.20 delay 2 seconds @14:46:39.23 PS.seek 0.965ml @14:47:29.08 delay 10 seconds @14:47:39.10 PRV.dial CBV @14:47:39.62 CTV.dial :air,avoiding: :intake @14:47:39.63 PSV.dial :air @14:47:40.11 PS.reconfigure PSconfig @14:47:40.16 PS.seek 2.5ml @14:47:56.48 delay 3 seconds @14:47:59.49 PSV.dial PTV @14:48:00.31 PTV.dial :PRV,avoiding: :puck @14:48:01.11 PS.seek 0ml @14:48:27.42 PSV.dial :air @14:48:28.73 PS.seek 2.5ml @14:48:55.14 delay 3 seconds @14:48:58.15 PSV.dial PTV @14:48:58.95 PTV.dial :PRV,avoiding: :puck @14:48:58.97 PS.seek 0ml @14:49:25.11 Pushing modified lysate into AIV Injection Loop @14:49:25.12 CBV.dial AIV @14:49:25.13 AIV.dial :bypass @14:49:25.14 PRV.dial CBV @14:49:25.16 PSV.dial :air @14:49:25.98 PS.seek 2.5ml @14:49:52.05 delay 3 seconds @14:49:55.06 PSV.dial :PML @14:49:55.56 PTV.dial :PRVmixing,avoiding: :puck @14:49:56.43 PS.reconfigure PSslow1 @14:49:56.48 PS.seek 1.74ml @14:51:13.86 delay 5 seconds @14:51:18.88 AIV.dial :sample @14:51:19.54 AWV.select :waste @14:51:19.72 PS.reconfigure PSslow2 @14:51:19.77 PS.seek 1.22ml @14:53:07.33 delay 15 seconds @14:53:22.35 AIV.dial :bypass @14:53:22.96 PS.reconfigure PSconfig @14:53:23.01 PS.seek 0ml @14:53:36.13 PSV.dial :air @14:53:36.63 PS.seek 2.5ml @14:54:02.76 PSV.dial :PML @14:54:03.27 PS.seek 0ml @14:54:29.41 AIV.dial :coilToMFB @14:54:29.43 AWV.select :air @14:54:29.60 Extracting Nucleic Acid from AIV @14:54:29.62
Beginning samplePassD @14:54:29.67 SPE.hold 2:46:40,55C @14:54:29.75 ASV.dial :SPEdelivery @14:54:29.83
AWV.select :air @14:54:29.84 AIV.dial :bypass @14:54:29.85 PSV.dial PSR @14:54:30.17 delay 20 seconds @14:54:31.06
Pulling 1.5ml of PSR.killMFB @14:54:31.07 PSR.select :killMFB @14:54:31.20 PS.seek 1.5ml @14:54:47.17 delay 5 seconds @14:54:50.20 primeLysateINT @14:54:50.21 AWV.select :air @14:54:50.22 AIV.dial :coilToMFB @14:54:50.23 ATV.dial AIV @14:54:52.00 AS.reconfigure ASslow1 @14:54:52.05 AS.seek 0.299ml @14:54:52.18
PSV.dial :waste1 @14:54:52.82 PS.seek 0ml @14:55:08.79 PSV.dial PSR @14:55:09.27 PSR.select :air @14:55:09.40 PS.seek 2.5ml @14:55:36.09 PRV.dial CBV @14:55:36.11
CBV.dial PRV @14:55:36.13 PSV.dial :mixloop @14:55:37.28 CTV.dial :air,avoiding: :intake @14:55:37.29 PTV.dial :PRVmixing @14:55:37.32 PS.seek 0ml @14:55:52.73 delay 10 seconds @14:56:02.75 ATV.dial :air2 @14:56:03.48
PSV.dial PSR @14:56:03.63 AS.seek 0.329ml @14:56:04.70
Pulling 1.5ml of PSR.flush @14:56:04.71 PSR.select :flush @14:56:04.84 PS.seek 1.5ml @14:56:09.95 ATV.dial :waste2 @14:56:10.51 AS.reconfigure ASnorm @14:56:10.56 AS.seek :empty @14:56:20.83
delay 5 seconds @14:56:25.86 PSV.dial :waste1 @14:56:26.36 PS.seek 0ml @14:56:27.57 Lysate primed! @14:56:27.58 AFV.select :holdingCoil @14:56:27.59 ATV.dial :air2 @14:56:28.22 AS.reconfigure ASslow1 @14:56:28.27 AS.seek 0.069ml @14:56:42.38
PSV.dial PSR @14:56:42.65 delay 3 seconds @14:56:42.90
PSR.select :air @14:56:43.03 PS.seek 2.5ml @14:56:45.67 ATV.dial :AIV @14:56:46.53 AS.seek 0.469ml @14:57:09.18 PRV.dial CBV @14:57:09.21
CBV.dial PRV @14:57:09.22 PSV.dial :mixloop @14:57:10.38 CTV.dial :air,avoiding: :intake @14:57:10.39 PTV.dial :PRVmixing @14:57:10.41 PS.seek 0ml @14:57:36.60 PSV.dial PSR @14:57:37.80 Pulling 1.5ml of PSR.flush @14:57:37.81 PSR.select :flush @14:57:37.94 PS.seek 1.5ml @14:57:53.93 delay 5 seconds @14:57:58.96 PSV.dial :waste1 @14:57:59.47 PS.seek 0ml @14:58:07.23 delay 5 seconds @14:58:12.31 Solid Phase Extraction Heater at 47C @14:58:12.32 ATV.dial ASV @14:58:12.73 ASV.dial :SPEdelivery @14:58:12.81 AS.seek 0.041ml @14:58:15.45
PSV.dial PSR @14:58:15.97 PSR.select :air @14:58:16.10 PS.seek 2.5ml @14:58:42.24 PRV.dial CBV @14:58:42.27
CBV.dial PRV @14:58:42.28 PSV.dial :mixloop @14:58:43.41 CTV.dial :air,avoiding: :intake @14:58:43.42 PTV.dial :PRVmixing @14:58:43.44 PS.seek 0ml @14:59:09.60 PSV.dial PSR @14:59:10.80 Pulling 1.5ml of PSR.flush @14:59:10.81 PSR.select :flush @14:59:10.94 PS.seek 1.5ml @14:59:27.05 delay 5 seconds @14:59:32.08 PSV.dial :waste1 @14:59:32.60 PS.seek 0ml @14:59:39.15 delay 10 seconds @14:59:48.57
PSV.dial PSR @14:59:49.07 PSR.select :air @14:59:49.17 rinseLysateLineINT @14:59:49.18 AFV.select :carrierSolution @14:59:49.23
PS.seek 2.5ml @14:59:49.28 delay 2 seconds @14:59:51.31 AS.reconfigure ASconfig @14:59:51.36 AS.seek 1ml @15:00:15.33 PRV.dial CBV @15:00:15.36
CBV.dial PRV @15:00:15.37 PSV.dial :mixloop @15:00:16.47 CTV.dial :air,avoiding: :intake @15:00:16.48 PTV.dial :PRVmixing @15:00:16.96 PS.reconfigure PSslow @15:00:17.09 PS.seek 1.2ml @15:00:43.90 PSV.dial :waste1 @15:00:45.33 PS.reconfigure PSconfig @15:00:45.38 PS.seek 0ml @15:00:46.75 delay 1 second @15:00:47.76 AFV.select :holdingCoil @15:00:47.87 delay 1 second @15:00:48.88 ATV.dial AIV @15:00:49.43 AIV.dial :coilToMFB @15:00:49.44 AWV.select :waste @15:00:49.55 AS.reconfigure ASslow0 @15:00:49.60 AS.seek 0ml @15:00:58.33
PSV.dial :air @15:00:59.59 PS.seek 2.5ml @15:01:25.75 PSV.dial :mixloop @15:01:26.26 PS.reconfigure PSslow @15:01:26.31 PS.seek 0ml @15:02:17.45 PSV.dial :air @15:02:17.94 PS.reconfigure PSconfig @15:02:17.99 PS.seek 2.5ml @15:02:30.47 ATV.dial :air2 @15:02:31.14 AS.reconfigure ASslow1 @15:02:31.20 AS.seek 0.05ml @15:02:41.52 ATV.dial :AIV @15:02:42.35 AS.seek 0.01ml @15:02:44.17
PSV.dial :mixloop @15:02:44.68 PS.reconfigure PSslow @15:02:44.73 PS.seek 0ml @15:02:50.69 ATV.dial :waste @15:02:52.28 AS.seek :empty @15:02:54.73 ethanol wash @15:02:54.74 ATV.dial :air2 @15:02:55.98 AS.reconfigure ASslow2 @15:02:56.04 AS.seek 0.009ml @15:03:06.59 delay 3 seconds @15:03:09.62 Pulling 0.4ml of Ethanol Wash @15:03:09.63 ATV.dial :ethanol @15:03:10.54 AS.reconfigure ASslow1 @15:03:10.60 AS.seek 0.409ml @15:03:35.81
Cleaning Collection Syringe @15:03:35.82 CSV.dial CSR @15:03:36.36 Pulling 4ml of CSR.flush @15:03:36.37 CSR.select :flush @15:03:36.50 CS.seek 4ml @15:03:47.68 delay 5 seconds @15:03:52.70 CSR.select :air @15:03:52.99 CS.seek 10ml @15:04:09.30 CSV.dial CTV @15:04:09.33
CTV.dial CSV @15:04:10.83 CBV.dial :waste1 @15:04:11.36 CS.seek 0ml @15:04:31.34 delay 3 seconds @15:04:34.36 ATV.dial :air1 @15:04:34.76 delay 3 seconds @15:04:37.79
CSV.dial CSR @15:04:37.91 AS.seek 0.434ml @15:04:38.35
Pulling 4ml of CSR.flush @15:04:38.36 CSR.select :flush @15:04:38.49 CS.seek 4ml @15:04:43.25 delay 3 seconds @15:04:46.27 ATV.dial :ethanol @15:04:46.88 AS.seek 0.419ml @15:04:49.77
delay 5 seconds @15:04:50.14 delay 3 seconds @15:04:53.17 ATV.dial ASV @15:04:54.46 AS.seek 0.092ml @15:04:54.78
CSR.select :air @15:04:54.92 CS.seek 10ml @15:05:11.18 CSV.dial CTV @15:05:11.21
CTV.dial CSV @15:05:11.71 CBV.dial :waste1 @15:05:11.73 CS.seek 0ml @15:05:38.36 Garaging FlushPuck in Collection Clamp @15:05:38.44 Elbow.reconfigure TightElbow @15:05:38.49 Elbow.seek CC @15:05:39.30 Elbow.reconfigure LooseElbow @15:05:39.42 Forearm.seek CC @15:05:40.43 Hand.close @15:05:41.16 CC.seek :open @15:05:45.53 Forearm.seek :retract @15:05:46.55 Elbow.reconfigure TightElbow @15:05:46.60 Elbow.seek :garage @15:05:49.60 Elbow.reconfigure LooseElbow @15:05:49.71 Forearm.reconfigure TightForearm @15:05:49.76 Forearm.seek :garage @15:05:50.93 Hand.open @15:05:51.73 Forearm.reconfigure LooseForearm @15:05:51.78 Forearm.seek :retract @15:05:52.77 Whole Cell Archive sampling 1500ml @15:05:52.79 Loading wcr puck from tube 4 into Collection Clamp @15:05:53.06 SC.reconfigure SCtight @15:05:53.11 SC.seek 4 @15:05:55.26 SC.reconfigure SCloose @15:05:55.49 Elbow.reconfigure TightElbow @15:05:55.55 Elbow.seek 4 @15:05:57.54 Forearm.reconfigure TightForearm @15:05:57.59 Forearm.seek 4 + 700 counts @15:05:58.75 SE.reconfigure SEpick @15:05:58.82 SE.seek beamBreak @15:06:00.44 delay 3 seconds @15:06:00.58 SE.configure SEconfig @15:06:00.76 Elbow.seek 4 @15:06:00.97 Forearm.seek 4 + 300 counts @15:06:01.21 Forearm.reconfigure LooseForearm @15:06:01.26 Hand.close @15:06:02.06 SE.seek :down @15:06:02.30 Forearm.seek :retract @15:06:03.26 Elbow.seek CC @15:06:03.45 ATV.dial :waste2 @15:06:03.52 SE.coast @15:06:04.34 AS.reconfigure ASconfig @15:06:04.39 AS.seek :empty @15:06:05.03 Elbow.reconfigure LooseElbow @15:06:05.14 Forearm.reconfigure TightForearm @15:06:05.19 Forearm.seek CC @15:06:06.48 CC.seek :closed @15:06:10.61 ATV.dial :air2 @15:06:10.75 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.34 @15:06:10.77 Hand.open @15:06:11.02 AS.reconfigure ASslow1 @15:06:11.08 AS.seek 1ml @15:06:11.61 Forearm.reconfigure LooseForearm @15:06:11.66 Forearm.seek :retract @15:06:12.67 SC.coast @15:06:12.71 collectSample 1500ml w/bubble point of 55psi @15:06:12.73 Priming 7ml @15:06:12.74 Intake.select :close @15:06:12.75 Exhaust.select :close @15:06:12.77 SS.hold @15:06:13.00 Calibrating pressure sensors @15:06:13.02 CTV.dial :air @15:06:14.47 SSV.dial :spit,avoiding: :exhaust @15:06:14.49 SS.reconfigure SSfast @15:06:14.54 SS.seek 5ml @15:06:17.20 SSV.dial CBV @15:06:17.23 CBV.dial SSV @15:06:18.79 delay 3.8 seconds @15:06:22.74 SSV.dial :spit @15:06:24.20 SS.seek 0ml @15:06:26.91 SSV.dial CBV @15:06:28.39 CTV.dial :intake @15:06:29.21 SSV.dial CBV @15:06:29.24 CBV.dial SSV @15:06:29.26 Allowing 40s for 7.0ml of remaining 7.0ml @15:06:29.27 Intake.select :open @15:06:31.45 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:06:31.47 SS.reconfigure SSinhale.with maxSpeed: 150 @15:06:31.52 SS.seek 7ml @15:06:35.10 Vacuum too high -- waiting up to 40s @15:06:37.50 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:06:37.52 SS.reconfigure SSinhale.with maxSpeed: 135 @15:06:37.57 SS.seek 7ml @15:06:41.27 Stroke completed -- waiting for pressure to equalize ... @15:06:46.44 Intake.select :close @15:06:46.60 SSV.dial :exhaust @15:06:47.44 Exhaust.select :open @15:06:47.55 SS.reconfigure SSexhale @15:06:47.60 SS.seek 0ml @15:06:51.44 Exhaust.select :close @15:06:51.56 Sampling 1500.0ml @15:06:51.58 Intake.select :close @15:06:51.59 Exhaust.select :close @15:06:51.60 SS.reconfigure SSconfig @15:06:51.65 SS.hold @15:06:51.87 CTV.dial :intake @15:06:51.89 SSV.dial CBV @15:06:51.92 CBV.dial SSV @15:06:53.34 Allowing 130s for 25.0ml of remaining 1500.0ml @15:06:53.35 Intake.select :open @15:06:55.54 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:06:55.56 SS.reconfigure SSinhale.with maxSpeed: 135 @15:06:55.61 SS.seek 25ml @15:06:59.51 Vacuum too high -- waiting up to 40s @15:07:01.41 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:07:01.43 SS.reconfigure SSinhale.with maxSpeed: 122 @15:07:01.48 SS.seek 25ml @15:07:04.26 Vacuum too high -- waiting up to 40s @15:07:05.91 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:07:05.93 SS.reconfigure SSinhale.with maxSpeed: 110 @15:07:05.98 SS.seek 25ml @15:07:08.57 Vacuum too high -- waiting up to 40s @15:07:10.18 Intake @33.5psia, vacuum from 20.0 to 24.0psi @15:07:10.20 SS.reconfigure SSinhale.with maxSpeed: 99 @15:07:10.25 SS.seek 25ml @15:07:13.04 Vacuum too high -- waiting up to 40s @15:07:14.67 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:07:14.70 SS.reconfigure SSinhale.with maxSpeed: 89 @15:07:14.75 SS.seek 25ml @15:07:17.60 Vacuum too high -- waiting up to 40s @15:07:19.05 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:07:19.07 SS.reconfigure SSinhale.with maxSpeed: 80 @15:07:19.11 SS.seek 25ml @15:07:21.57 Vacuum too high -- waiting up to 40s @15:07:22.93 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:07:22.96 SS.reconfigure SSinhale.with maxSpeed: 72 @15:07:23.00 SS.seek 25ml @15:07:26.51 Vacuum too high -- waiting up to 40s @15:07:28.21 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:07:28.23 SS.reconfigure SSinhale.with maxSpeed: 65 @15:07:28.28 SS.seek 25ml @15:07:32.82 Vacuum too high -- waiting up to 40s @15:07:34.19 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:07:34.21 SS.reconfigure SSinhale.with maxSpeed: 59 @15:07:34.26 SS.seek 25ml @15:07:37.24 Stroke completed -- waiting for pressure to equalize ... @15:07:39.86 Intake.select :close @15:07:40.03 SSV.dial :exhaust @15:07:40.87 Exhaust.select :open @15:07:40.98 SS.reconfigure SSexhale @15:07:41.03 SS.seek 0ml @15:07:50.10 Exhaust.select :close @15:07:50.22 SSV.dial CBV @15:07:50.25 CBV.dial SSV @15:07:51.12 Allowing 130s for 25.0ml of remaining 1475.0ml @15:07:51.13 Intake.select :open @15:07:53.32 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:07:53.34 SS.reconfigure SSinhale.with maxSpeed: 59 @15:07:53.39 SS.seek 25ml @15:07:59.67 Vacuum too high -- waiting up to 40s @15:08:00.53 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:08:00.55 SS.reconfigure SSinhale.with maxSpeed: 53 @15:08:00.60 SS.seek 25ml @15:08:11.17 Vacuum too high -- waiting up to 40s @15:08:12.28 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:08:12.30 SS.reconfigure SSinhale.with maxSpeed: 48 @15:08:12.35 SS.seek 25ml @15:08:31.02 Stroke completed -- waiting for pressure to equalize ... @15:08:34.28 Intake.select :close @15:08:34.44 SSV.dial :exhaust @15:08:35.27 Exhaust.select :open @15:08:35.39 SS.reconfigure SSexhale @15:08:35.44 SS.seek 0ml @15:08:44.50 Exhaust.select :close @15:08:44.62 SSV.dial CBV @15:08:44.65 CBV.dial SSV @15:08:45.51 Allowing 130s for 25.0ml of remaining 1450.0ml @15:08:45.52 Intake.select :open @15:08:47.70 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:08:47.72 SS.reconfigure SSinhale.with maxSpeed: 48 @15:08:47.77 SS.seek 25ml @15:09:19.07 Vacuum too high -- waiting up to 40s @15:09:20.20 Intake @33.4psia, vacuum from 20.0 to 24.0psi @15:09:20.22 SS.reconfigure SSinhale.with maxSpeed: 43 @15:09:20.27 SS.seek 25ml @15:09:28.10 Stroke completed -- waiting for pressure to equalize ... @15:09:30.70 Intake.select :close @15:09:30.86 SSV.dial :exhaust @15:09:31.72 Exhaust.select :open @15:09:31.83 SS.reconfigure SSexhale @15:09:31.88 SS.seek 0ml @15:09:32.61 delay 5 seconds @15:09:37.63 ATV.dial ASV @15:09:38.59 ASV.dial :SPEdelivery @15:09:38.61 AS.seek 0.02ml @15:09:41.14 Exhaust.select :close @15:09:41.26 SSV.dial CBV @15:09:41.29 CBV.dial SSV @15:09:42.14 Allowing 130s for 25.0ml of remaining 1425.0ml @15:09:42.15 Intake.select :open @15:09:44.34 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:09:44.36 SS.reconfigure SSinhale.with maxSpeed: 43 @15:09:44.41 SS.seek 25ml @15:10:25.60 Stroke completed -- waiting for pressure to equalize ... @15:10:28.85 Intake.select :close @15:10:29.02 SSV.dial :exhaust @15:10:29.86 Exhaust.select :open @15:10:29.97 SS.reconfigure SSexhale @15:10:30.02 SS.seek 0ml @15:10:39.35 Exhaust.select :close @15:10:39.47 SSV.dial CBV @15:10:39.50 CBV.dial SSV @15:10:40.37 Allowing 130s for 25.0ml of remaining 1400.0ml @15:10:40.38 Intake.select :open @15:10:42.56 Intake @34.0psia, vacuum from 20.0 to 24.0psi @15:10:42.58 SS.reconfigure SSinhale.with maxSpeed: 43 @15:10:42.63 SS.seek 25ml @15:11:24.25 Stroke completed -- waiting for pressure to equalize ... @15:11:26.86 Intake.select :close @15:11:27.02 SSV.dial :exhaust @15:11:27.86 Exhaust.select :open @15:11:27.97 SS.reconfigure SSexhale @15:11:28.02 SS.seek 0ml @15:11:36.96 Exhaust.select :close @15:11:37.08 SSV.dial CBV @15:11:37.11 CBV.dial SSV @15:11:37.98 Allowing 130s for 25.0ml of remaining 1375.0ml @15:11:37.99 Intake.select :open @15:11:40.18 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:11:40.20 SS.reconfigure SSinhale.with maxSpeed: 43 @15:11:40.25 SS.seek 25ml @15:12:21.99 Stroke completed -- waiting for pressure to equalize ... @15:12:25.22 Intake.select :close @15:12:25.39 SSV.dial :exhaust @15:12:26.23 Exhaust.select :open @15:12:26.34 SS.reconfigure SSexhale @15:12:26.39 SS.seek 0ml @15:12:35.57 Exhaust.select :close @15:12:35.69 SSV.dial CBV @15:12:35.72 CBV.dial SSV @15:12:37.15 Allowing 130s for 25.0ml of remaining 1350.0ml @15:12:37.16 Intake.select :open @15:12:39.34 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:12:39.36 SS.reconfigure SSinhale.with maxSpeed: 43 @15:12:39.41 SS.seek 25ml @15:12:55.99 delay 5 seconds @15:13:01.01 ATV.dial :air2 @15:13:02.06 AS.seek 0.52ml @15:13:20.72 Stroke completed -- waiting for pressure to equalize ... @15:13:23.96 Intake.select :close @15:13:24.12 SSV.dial :exhaust @15:13:24.97 Exhaust.select :open @15:13:25.08 SS.reconfigure SSexhale @15:13:25.13 SS.seek 0ml @15:13:34.19 Exhaust.select :close @15:13:34.31 SSV.dial CBV @15:13:34.34 CBV.dial SSV @15:13:35.20 Allowing 130s for 25.0ml of remaining 1325.0ml @15:13:35.21 Intake.select :open @15:13:37.40 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:13:37.42 SS.reconfigure SSinhale.with maxSpeed: 43 @15:13:37.47 SS.seek 25ml @15:14:18.96 Stroke completed -- waiting for pressure to equalize ... @15:14:22.20 Intake.select :close @15:14:22.37 SSV.dial :exhaust @15:14:23.22 Exhaust.select :open @15:14:23.33 SS.reconfigure SSexhale @15:14:23.38 SS.seek 0ml @15:14:32.49 Exhaust.select :close @15:14:32.61 SSV.dial CBV @15:14:32.64 CBV.dial SSV @15:14:33.50 Allowing 130s for 25.0ml of remaining 1300.0ml @15:14:33.51 Intake.select :open @15:14:35.70 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:14:35.72 SS.reconfigure SSinhale.with maxSpeed: 43 @15:14:35.77 SS.seek 25ml @15:14:42.94 delay 5 seconds @15:14:47.97 ATV.dial ASV @15:14:48.94 ASV.dial :wasteR @15:14:49.31 AS.seek 0.3ml @15:15:17.10 Stroke completed -- waiting for pressure to equalize ... @15:15:20.32 Intake.select :close @15:15:20.49 SSV.dial :exhaust @15:15:21.33 Exhaust.select :open @15:15:21.45 SS.reconfigure SSexhale @15:15:21.50 SS.seek 0ml @15:15:30.89 Exhaust.select :close @15:15:31.01 SSV.dial CBV @15:15:31.04 CBV.dial SSV @15:15:31.90 Allowing 130s for 25.0ml of remaining 1275.0ml @15:15:31.91 Intake.select :open @15:15:33.80 delay 20 seconds @15:15:34.09 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:15:34.11 SS.reconfigure SSinhale.with maxSpeed: 43 @15:15:34.16 SS.seek 25ml @15:15:53.83 AS.seek 0.07ml @15:16:15.66 Stroke completed -- waiting for pressure to equalize ... @15:16:18.83 Intake.select :close @15:16:18.99 SSV.dial :exhaust @15:16:19.83 Exhaust.select :open @15:16:19.95 SS.reconfigure SSexhale @15:16:20.00 SS.seek 0ml @15:16:29.18 Exhaust.select :close @15:16:29.30 SSV.dial CBV @15:16:29.33 CBV.dial SSV @15:16:30.20 Allowing 130s for 25.0ml of remaining 1250.0ml @15:16:30.21 Intake.select :open @15:16:32.40 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:16:32.42 SS.reconfigure SSinhale.with maxSpeed: 43 @15:16:32.47 SS.seek 25ml @15:16:40.35 delay 20 seconds @15:17:00.37 ATV.dial :waste2 @15:17:01.13 AS.reconfigure ASconfig @15:17:01.18 AS.seek :empty @15:17:05.56 Elution @15:17:05.57 ATV.dial :air1 @15:17:06.70 AS.reconfigure ASslow1 @15:17:06.75 AS.seek 0.049ml @15:17:13.74 Stroke completed -- waiting for pressure to equalize ... @15:17:16.98 Intake.select :close @15:17:17.16 delay 3 seconds @15:17:17.18 SSV.dial :exhaust @15:17:18.03 Exhaust.select :open @15:17:18.14 SS.reconfigure SSexhale @15:17:18.19 SS.seek 0ml @15:17:20.18 ATV.dial :ElutionWater @15:17:20.85 AS.seek 0.109ml @15:17:27.27 Exhaust.select :close @15:17:27.39 SSV.dial CBV @15:17:27.42 CBV.dial SSV @15:17:28.29 Allowing 130s for 25.0ml of remaining 1225.0ml @15:17:28.30 Intake.select :open @15:17:30.49 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:17:30.51 SS.reconfigure SSinhale.with maxSpeed: 43 @15:17:30.56 SS.seek 25ml @15:17:33.21 delay 3 seconds @15:17:36.24 ATV.dial ASV,via: :air2 @15:17:38.31 ASV.dial :SPEelution @15:17:38.88 AFV.select :carrierSolution @15:17:39.05 AS.reconfigure ASnorm @15:17:39.10 AS.seek 0.759ml @15:18:11.83 Stroke completed -- waiting for pressure to equalize ... @15:18:12.14 delay 3 seconds @15:18:15.06 Intake.select :close @15:18:15.15 AFV.select :holdingCoil @15:18:15.25 SSV.dial :exhaust @15:18:15.37 AS.reconfigure ASslow1 @15:18:15.42 AS.seek 0.474ml @15:18:16.11 Exhaust.select :open @15:18:16.22 SS.reconfigure SSexhale @15:18:16.27 SS.seek 0ml @15:18:25.46 Exhaust.select :close @15:18:25.58 SSV.dial CBV @15:18:25.61 CBV.dial SSV @15:18:26.47 Allowing 130s for 25.0ml of remaining 1200.0ml @15:18:26.48 Intake.select :open @15:18:28.67 Intake @33.7psia, vacuum from 20.0 to 24.0psi @15:18:28.69 SS.reconfigure SSinhale.with maxSpeed: 43 @15:18:28.74 SS.seek 25ml @15:19:10.03 Stroke completed -- waiting for pressure to equalize ... @15:19:13.05 delay 5 seconds @15:19:13.88 Intake.select :close @15:19:14.04 SSV.dial :exhaust @15:19:14.89 Exhaust.select :open @15:19:15.00 SS.reconfigure SSexhale @15:19:15.07 SS.seek 0ml @15:19:18.16 AS.reconfigure ASslow2 @15:19:18.21 AS.seek 0.439ml @15:19:23.86 Exhaust.select :close @15:19:23.98 SSV.dial CBV @15:19:24.01 CBV.dial SSV @15:19:24.87 Allowing 130s for 25.0ml of remaining 1175.0ml @15:19:24.88 Intake.select :open @15:19:27.07 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:19:27.09 SS.reconfigure SSinhale.with maxSpeed: 43 @15:19:27.14 SS.seek 25ml @15:19:53.86 delay 1:00 @15:20:09.07 Stroke completed -- waiting for pressure to equalize ... @15:20:12.97 Intake.select :close @15:20:13.14 SSV.dial :exhaust @15:20:13.98 Exhaust.select :open @15:20:14.09 SS.reconfigure SSexhale @15:20:14.15 SS.seek 0ml @15:20:23.39 Exhaust.select :close @15:20:23.51 SSV.dial CBV @15:20:23.54 CBV.dial SSV @15:20:24.40 Allowing 130s for 25.0ml of remaining 1150.0ml @15:20:24.41 Intake.select :open @15:20:26.59 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:20:26.61 SS.reconfigure SSinhale.with maxSpeed: 43 @15:20:26.67 SS.seek 25ml @15:20:53.89 pushing eluate to holding coil @15:20:53.96 AS.seek 0.279ml @15:21:08.18 Stroke completed -- waiting for pressure to equalize ... @15:21:12.07 Intake.select :close @15:21:12.23 SSV.dial :exhaust @15:21:13.08 Exhaust.select :open @15:21:13.19 SS.reconfigure SSexhale @15:21:13.26 SS.seek 0ml @15:21:22.35 Exhaust.select :close @15:21:22.47 SSV.dial CBV @15:21:22.50 CBV.dial SSV @15:21:23.37 Allowing 130s for 25.0ml of remaining 1125.0ml @15:21:23.38 Intake.select :open @15:21:25.57 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:21:25.59 SS.reconfigure SSinhale.with maxSpeed: 43 @15:21:25.64 SS.seek 25ml @15:22:06.68 Stroke completed -- waiting for pressure to equalize ... @15:22:10.56 Intake.select :close @15:22:10.72 SSV.dial :exhaust @15:22:11.58 Exhaust.select :open @15:22:11.69 SS.reconfigure SSexhale @15:22:11.75 SS.seek 0ml @15:22:21.55 Exhaust.select :close @15:22:21.67 SSV.dial CBV @15:22:21.70 CBV.dial SSV @15:22:22.58 Allowing 130s for 25.0ml of remaining 1100.0ml @15:22:22.59 Intake.select :open @15:22:24.77 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:22:24.79 SS.reconfigure SSinhale.with maxSpeed: 43 @15:22:24.84 SS.seek 25ml @15:22:50.10 Vacuum too high -- waiting up to 40s @15:22:51.03 Intake @33.6psia, vacuum from 20.0 to 24.0psi @15:22:51.05 SS.reconfigure SSinhale.with maxSpeed: 39 @15:22:51.10 SS.seek 25ml @15:23:09.27 Stroke completed -- waiting for pressure to equalize ... @15:23:12.51 Intake.select :close @15:23:12.67 SSV.dial :exhaust @15:23:13.51 Exhaust.select :open @15:23:13.62 SS.reconfigure SSexhale @15:23:13.67 SS.seek 0ml @15:23:22.96 Exhaust.select :close @15:23:23.08 SSV.dial CBV @15:23:23.13 CBV.dial SSV @15:23:23.97 Allowing 130s for 25.0ml of remaining 1075.0ml @15:23:23.98 Intake.select :open @15:23:26.17 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:23:26.19 SS.reconfigure SSinhale.with maxSpeed: 39 @15:23:26.24 SS.seek 25ml @15:23:34.93 delay 30 seconds @15:24:04.96 ATV.dial :waste,via: :air3 @15:24:06.74 AS.reconfigure ASconfig @15:24:06.79 AS.seek :empty @15:24:11.80 Stroke completed -- waiting for pressure to equalize ... @15:24:15.68 Intake.select :close @15:24:15.85 SSV.dial :exhaust @15:24:16.69 Exhaust.select :open @15:24:16.81 SS.reconfigure SSexhale @15:24:16.87 SS.seek 0ml @15:24:23.19 Parking Bleach in LysateSource line and SPEcolumn @15:24:23.20 ATV.dial :air3 @15:24:24.10 AS.reconfigure ASslow1 @15:24:24.15 AS.seek 0.029ml @15:24:26.10 Exhaust.select :close @15:24:26.22 SSV.dial CBV @15:24:26.25 CBV.dial SSV @15:24:27.12 Allowing 130s for 25.0ml of remaining 1050.0ml @15:24:27.13 Intake.select :open @15:24:29.32 Intake @33.8psia, vacuum from 20.0 to 24.0psi @15:24:29.34 SS.reconfigure SSinhale.with maxSpeed: 39 @15:24:29.39 SS.seek 25ml @15:24:30.48 ATV.dial :ElutionWater @15:24:31.09 AS.seek 0.059ml @15:24:37.39 ATV.dial :air3 @15:24:38.01 AS.seek 0.069ml @15:24:40.29 delay 2 seconds @15:24:42.30 ATV.dial :bleach @15:24:42.79 AS.seek 0.769ml @15:25:14.91 Stroke completed -- waiting for pressure to equalize ... @15:25:18.71 Intake.select :close @15:25:18.88 SSV.dial :exhaust @15:25:19.73 Exhaust.select :open @15:25:19.84 SS.reconfigure SSexhale @15:25:19.89 SS.seek 0ml @15:25:29.13 Exhaust.select :close @15:25:29.25 SSV.dial CBV @15:25:29.28 CBV.dial SSV @15:25:30.15 Allowing 130s for 25.0ml of remaining 1025.0ml @15:25:30.16 Intake.select :open @15:25:32.35 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:25:32.37 SS.reconfigure SSinhale.with maxSpeed: 39 @15:25:32.42 SS.seek 25ml @15:26:18.07 Stroke completed -- waiting for pressure to equalize ... @15:26:21.95 Intake.select :close @15:26:22.12 SSV.dial :exhaust @15:26:22.98 Exhaust.select :open @15:26:23.09 SS.reconfigure SSexhale @15:26:23.16 SS.seek 0ml @15:26:32.25 Exhaust.select :close @15:26:32.37 SSV.dial CBV @15:26:32.40 CBV.dial SSV @15:26:33.25 Allowing 130s for 25.0ml of remaining 1000.0ml @15:26:33.26 Intake.select :open @15:26:35.45 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:26:35.47 SS.reconfigure SSinhale.with maxSpeed: 39 @15:26:35.52 SS.seek 25ml @15:27:03.82 delay 3 seconds @15:27:06.84 ATV.dial :air3 @15:27:07.40 delay 3 seconds @15:27:10.49 AS.seek 0.794ml @15:27:15.72 delay 3 seconds @15:27:18.74 ATV.dial :bleach @15:27:19.37 AS.seek 0.779ml @15:27:21.08 Stroke completed -- waiting for pressure to equalize ... @15:27:22.65 delay 3 seconds @15:27:24.96 Intake.select :close @15:27:25.13 SSV.dial :exhaust @15:27:25.67 ATV.dial :AIV @15:27:25.97 Exhaust.select :open @15:27:26.08 SS.reconfigure SSexhale @15:27:26.15 SS.seek 0ml @15:27:26.91 AS.reconfigure ASconfig @15:27:26.96 AS.seek 0.015ml @15:27:35.46 Exhaust.select :close @15:27:35.58 SSV.dial CBV @15:27:35.61 CBV.dial SSV @15:27:36.48 Allowing 130s for 25.0ml of remaining 975.0ml @15:27:36.49 Intake.select :open @15:27:38.68 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:27:38.70 SS.reconfigure SSinhale.with maxSpeed: 39 @15:27:38.75 SS.seek 25ml @15:28:11.18 ATV.dial :waste @15:28:12.74 AS.seek :empty @15:28:13.96 ATV.dial :air3 @15:28:14.64 AS.reconfigure ASslow1 @15:28:14.69 AS.seek 0.009ml @15:28:17.07 delay 2 seconds @15:28:19.08 ATV.dial :bleach @15:28:19.56 AS.seek 0.579ml @15:28:24.22 Stroke completed -- waiting for pressure to equalize ... @15:28:28.16 Intake.select :close @15:28:28.32 SSV.dial :exhaust @15:28:29.17 Exhaust.select :open @15:28:29.28 SS.reconfigure SSexhale @15:28:29.35 SS.seek 0ml @15:28:38.57 Exhaust.select :close @15:28:38.69 SSV.dial CBV @15:28:38.72 CBV.dial SSV @15:28:39.59 Allowing 130s for 25.0ml of remaining 950.0ml @15:28:39.60 Intake.select :open @15:28:41.79 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:28:41.81 SS.reconfigure SSinhale.with maxSpeed: 39 @15:28:41.86 SS.seek 25ml @15:29:27.69 Stroke completed -- waiting for pressure to equalize ... @15:29:31.57 Intake.select :close @15:29:31.73 SSV.dial :exhaust @15:29:32.58 Exhaust.select :open @15:29:32.69 SS.reconfigure SSexhale @15:29:32.75 SS.seek 0ml @15:29:41.61 Exhaust.select :close @15:29:41.73 SSV.dial CBV @15:29:41.76 CBV.dial SSV @15:29:42.62 Allowing 130s for 25.0ml of remaining 925.0ml @15:29:42.63 Intake.select :open @15:29:44.82 Intake @34.2psia, vacuum from 20.0 to 24.0psi @15:29:44.84 SS.reconfigure SSinhale.with maxSpeed: 39 @15:29:44.88 SS.seek 25ml @15:30:14.46 delay 3 seconds @15:30:17.48 ATV.dial :air3 @15:30:18.04 delay 3 seconds @15:30:21.13 AS.seek 0.604ml @15:30:26.45 delay 3 seconds @15:30:29.47 ATV.dial :bleach @15:30:30.11 AS.seek 0.589ml @15:30:30.61 Stroke completed -- waiting for pressure to equalize ... @15:30:33.40 delay 3 seconds @15:30:35.15 Intake.select :close @15:30:35.31 SSV.dial :exhaust @15:30:36.15 Exhaust.select :open @15:30:36.26 SS.reconfigure SSexhale @15:30:36.31 SS.seek 0ml @15:30:36.42 ATV.dial :ASV @15:30:37.43 ASV.dial :wasteR @15:30:38.10 AS.seek 0ml @15:30:45.41 Exhaust.select :close @15:30:45.53 SSV.dial CBV @15:30:45.56 CBV.dial SSV @15:30:46.44 Allowing 130s for 25.0ml of remaining 900.0ml @15:30:46.45 Intake.select :open @15:30:48.63 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:30:48.65 SS.reconfigure SSinhale.with maxSpeed: 39 @15:30:48.70 SS.seek 25ml @15:31:34.00 Stroke completed -- waiting for pressure to equalize ... @15:31:38.54 Intake.select :close @15:31:38.70 SSV.dial :exhaust @15:31:39.55 Exhaust.select :open @15:31:39.67 SS.reconfigure SSexhale @15:31:39.72 SS.seek 0ml @15:31:48.86 Exhaust.select :close @15:31:48.98 SSV.dial CBV @15:31:49.01 CBV.dial SSV @15:31:49.87 Allowing 130s for 25.0ml of remaining 875.0ml @15:31:49.88 Intake.select :open @15:31:52.07 Intake @33.9psia, vacuum from 20.0 to 24.0psi @15:31:52.09 SS.reconfigure SSinhale.with maxSpeed: 39 @15:31:52.14 SS.seek 25ml @15:32:36.87 delay 1:00 @15:32:37.88 Stroke completed -- waiting for pressure to equalize ... @15:32:42.39 Intake.select :close @15:32:42.55 SSV.dial :exhaust @15:32:43.40 Exhaust.select :open @15:32:43.51 SS.reconfigure SSexhale @15:32:43.57 SS.seek 0ml @15:32:52.35 Exhaust.select :close @15:32:52.47 SSV.dial CBV @15:32:52.50 CBV.dial SSV @15:32:53.37 Allowing 130s for 25.0ml of remaining 850.0ml @15:32:53.38 Intake.select :open @15:32:55.56 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:32:55.58 SS.reconfigure SSinhale.with maxSpeed: 39 @15:32:55.63 SS.seek 25ml @15:33:27.70 Vacuum too high -- waiting up to 40s @15:33:28.82 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:33:28.84 SS.reconfigure SSinhale.with maxSpeed: 35 @15:33:28.89 SS.seek 25ml @15:33:36.89 AFV.select :carrierSolution @15:33:37.00 delay 2 seconds @15:33:39.03 AS.reconfigure ASconfig @15:33:39.07 AS.seek 1ml @15:33:44.38 Stroke completed -- waiting for pressure to equalize ... @15:33:48.28 Intake.select :close @15:33:48.44 SSV.dial :exhaust @15:33:49.29 Exhaust.select :open @15:33:49.40 SS.reconfigure SSexhale @15:33:49.46 SS.seek 0ml @15:33:58.66 Exhaust.select :close @15:33:58.79 SSV.dial CBV @15:33:58.82 CBV.dial SSV @15:33:59.67 Allowing 130s for 25.0ml of remaining 825.0ml @15:33:59.68 Intake.select :open @15:34:01.87 Intake @34.5psia, vacuum from 20.0 to 24.0psi @15:34:01.89 SS.reconfigure SSinhale.with maxSpeed: 35 @15:34:01.93 SS.seek 25ml @15:34:36.84 delay 1 second @15:34:37.85 AFV.select :holdingCoil @15:34:37.96 delay 1 second @15:34:38.97 ATV.dial ASV @15:34:39.53 ASV.dial :wasteR @15:34:39.57 AS.reconfigure ASslow1 @15:34:39.62 AS.seek 0ml @15:34:52.39 Stroke completed -- waiting for pressure to equalize ... @15:34:56.26 Intake.select :close @15:34:56.43 SSV.dial :exhaust @15:34:57.26 Exhaust.select :open @15:34:57.37 SS.reconfigure SSexhale @15:34:57.43 SS.seek 0ml @15:35:06.58 Exhaust.select :close @15:35:06.70 SSV.dial CBV @15:35:06.73 CBV.dial SSV @15:35:07.61 Allowing 130s for 25.0ml of remaining 800.0ml @15:35:07.62 Intake.select :open @15:35:09.81 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:35:09.83 SS.reconfigure SSinhale.with maxSpeed: 35 @15:35:09.88 SS.seek 25ml @15:36:00.72 Stroke completed -- waiting for pressure to equalize ... @15:36:04.61 Intake.select :close @15:36:04.77 SSV.dial :exhaust @15:36:05.61 Exhaust.select :open @15:36:05.72 SS.reconfigure SSexhale @15:36:05.82 SS.seek 0ml @15:36:15.25 Exhaust.select :close @15:36:15.37 SSV.dial CBV @15:36:15.40 CBV.dial SSV @15:36:16.26 Allowing 130s for 25.0ml of remaining 775.0ml @15:36:16.27 Intake.select :open @15:36:18.45 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:36:18.47 SS.reconfigure SSinhale.with maxSpeed: 35 @15:36:18.52 SS.seek 25ml @15:37:09.08 Stroke completed -- waiting for pressure to equalize ... @15:37:13.59 Intake.select :close @15:37:13.76 SSV.dial :exhaust @15:37:14.62 Exhaust.select :open @15:37:14.74 SS.reconfigure SSexhale @15:37:14.79 SS.seek 0ml @15:37:24.08 Exhaust.select :close @15:37:24.20 SSV.dial CBV @15:37:24.23 CBV.dial SSV @15:37:25.08 Allowing 130s for 25.0ml of remaining 750.0ml @15:37:25.09 Intake.select :open @15:37:27.28 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:37:27.30 SS.reconfigure SSinhale.with maxSpeed: 35 @15:37:27.35 SS.seek 25ml @15:38:01.01 delay 20 seconds @15:38:17.74 Stroke completed -- waiting for pressure to equalize ... @15:38:21.02 ATV.dial :air3 @15:38:22.19 Intake.select :close @15:38:22.35 SSV.dial :exhaust @15:38:22.52 AS.seek 0.59ml @15:38:23.19 Exhaust.select :open @15:38:23.31 SS.reconfigure SSexhale @15:38:23.37 SS.seek 0ml @15:38:32.69 Exhaust.select :close @15:38:32.81 SSV.dial CBV @15:38:32.84 CBV.dial SSV @15:38:33.72 Allowing 130s for 25.0ml of remaining 725.0ml @15:38:33.73 Intake.select :open @15:38:35.92 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:38:35.94 SS.reconfigure SSinhale.with maxSpeed: 35 @15:38:35.99 SS.seek 25ml @15:39:26.28 Stroke completed -- waiting for pressure to equalize ... @15:39:30.81 Intake.select :close @15:39:30.98 SSV.dial :exhaust @15:39:31.84 Exhaust.select :open @15:39:31.96 SS.reconfigure SSexhale @15:39:32.01 SS.seek 0ml @15:39:41.21 Exhaust.select :close @15:39:41.33 SSV.dial CBV @15:39:41.36 CBV.dial SSV @15:39:42.23 Allowing 130s for 25.0ml of remaining 700.0ml @15:39:42.24 Intake.select :open @15:39:44.42 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:39:44.45 SS.reconfigure SSinhale.with maxSpeed: 35 @15:39:44.50 SS.seek 25ml @15:40:21.48 delay 3 seconds @15:40:24.50 ATV.dial :ASV @15:40:25.98 ASV.dial :wasteR @15:40:26.07 AS.seek 0.09ml @15:40:35.00 Stroke completed -- waiting for pressure to equalize ... @15:40:39.50 Intake.select :close @15:40:39.67 SSV.dial :exhaust @15:40:40.52 Exhaust.select :open @15:40:40.63 SS.reconfigure SSexhale @15:40:40.68 SS.seek 0ml @15:40:49.85 Exhaust.select :close @15:40:49.97 SSV.dial CBV @15:40:50.00 CBV.dial SSV @15:40:50.87 Allowing 130s for 25.0ml of remaining 675.0ml @15:40:50.88 Intake.select :open @15:40:53.06 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:40:53.08 SS.reconfigure SSinhale.with maxSpeed: 35 @15:40:53.13 SS.seek 25ml @15:41:43.46 Stroke completed -- waiting for pressure to equalize ... @15:41:47.98 Intake.select :close @15:41:48.14 SSV.dial :exhaust @15:41:49.56 Exhaust.select :open @15:41:49.67 SS.reconfigure SSexhale @15:41:49.73 SS.seek 0ml @15:41:58.66 Exhaust.select :close @15:41:58.78 SSV.dial CBV @15:41:58.83 CBV.dial SSV @15:41:59.69 Allowing 130s for 25.0ml of remaining 650.0ml @15:41:59.70 Intake.select :open @15:42:01.89 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:42:01.91 SS.reconfigure SSinhale.with maxSpeed: 35 @15:42:01.96 SS.seek 25ml @15:42:06.90 delay 30 seconds @15:42:36.92 ATV.dial :extSrc @15:42:37.68 delay 3 seconds @15:42:40.70 ATV.dial :ElutionWater @15:42:41.81 AS.seek 0.12ml @15:42:48.14 ATV.dial :air3 @15:42:48.77 AS.seek 0.13ml @15:42:51.07 delay 2 seconds @15:42:52.68 Stroke completed -- waiting for pressure to equalize ... @15:42:53.08 ATV.dial :bleach @15:42:53.56 AS.seek 0.23ml @15:42:57.83 Intake.select :close @15:42:57.99 SSV.dial :exhaust @15:42:58.85 Exhaust.select :open @15:42:58.96 SS.reconfigure SSexhale @15:42:59.01 SS.seek 0ml @15:43:07.94 Exhaust.select :close @15:43:08.06 SSV.dial CBV @15:43:08.09 CBV.dial SSV @15:43:08.95 Allowing 130s for 25.0ml of remaining 625.0ml @15:43:08.96 Intake.select :open @15:43:11.15 Intake @34.9psia, vacuum from 20.0 to 24.0psi @15:43:11.17 SS.reconfigure SSinhale.with maxSpeed: 35 @15:43:11.22 SS.seek 25ml @15:43:14.06 delay 3 seconds @15:43:17.08 ATV.dial :air3 @15:43:17.64 delay 3 seconds @15:43:20.72 AS.seek 0.255ml @15:43:26.06 delay 3 seconds @15:43:29.08 ATV.dial :bleach @15:43:29.71 AS.seek 0.24ml @15:43:33.07 delay 3 seconds @15:43:36.09 ATV.dial :ASV @15:43:37.12 ASV.dial :SPEdelivery @15:43:37.48 AFV.select :carrierSolution @15:43:37.60 delay 2 seconds @15:43:39.63 AS.reconfigure ASconfig @15:43:39.68 AS.seek 0.5ml @15:43:54.90 delay 1 second @15:43:55.91 AFV.select :holdingCoil @15:43:56.07 AS.reconfigure ASslow1 @15:43:56.12 AS.seek 0.17ml @15:43:56.70 Vacuum too high -- waiting up to 40s @15:43:57.82 Intake @34.1psia, vacuum from 20.0 to 24.0psi @15:43:57.84 SS.reconfigure SSinhale.with maxSpeed: 32 @15:43:57.89 SS.seek 25ml @15:44:04.00 Stroke completed -- waiting for pressure to equalize ... @15:44:09.08 Intake.select :close @15:44:09.25 SSV.dial :exhaust @15:44:10.11 Exhaust.select :open @15:44:10.23 SS.reconfigure SSexhale @15:44:10.29 SS.seek 0ml @15:44:19.66 Exhaust.select :close @15:44:19.78 SSV.dial CBV @15:44:19.81 CBV.dial SSV @15:44:20.67 Allowing 130s for 25.0ml of remaining 600.0ml @15:44:20.68 Intake.select :open @15:44:22.86 Intake @34.9psia, vacuum from 20.0 to 24.0psi @15:44:22.88 SS.reconfigure SSinhale.with maxSpeed: 32 @15:44:22.93 SS.seek 25ml @15:45:02.80 SPE.hold 2:46:40,95C @15:45:02.86 delay 3:00 @15:45:17.88 Stroke completed -- waiting for pressure to equalize ... @15:45:22.40 Intake.select :close @15:45:22.56 SSV.dial :exhaust @15:45:23.43 Exhaust.select :open @15:45:23.54 SS.reconfigure SSexhale @15:45:23.60 SS.seek 0ml @15:45:32.77 Exhaust.select :close @15:45:32.89 SSV.dial CBV @15:45:32.92 CBV.dial SSV @15:45:33.81 Allowing 130s for 25.0ml of remaining 575.0ml @15:45:33.82 Intake.select :open @15:45:36.01 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:45:36.03 SS.reconfigure SSinhale.with maxSpeed: 32 @15:45:36.08 SS.seek 25ml @15:46:31.27 Stroke completed -- waiting for pressure to equalize ... @15:46:35.78 Intake.select :close @15:46:35.95 SSV.dial :exhaust @15:46:36.82 Exhaust.select :open @15:46:36.94 SS.reconfigure SSexhale @15:46:37.00 SS.seek 0ml @15:46:46.60 Exhaust.select :close @15:46:46.72 SSV.dial CBV @15:46:46.75 CBV.dial SSV @15:46:47.64 Allowing 130s for 25.0ml of remaining 550.0ml @15:46:47.65 Intake.select :open @15:46:49.84 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:46:49.86 SS.reconfigure SSinhale.with maxSpeed: 32 @15:46:49.91 SS.seek 25ml @15:47:45.11 Stroke completed -- waiting for pressure to equalize ... @15:47:49.65 Intake.select :close @15:47:49.81 SSV.dial :exhaust @15:47:50.69 Exhaust.select :open @15:47:50.80 SS.reconfigure SSexhale @15:47:50.85 SS.seek 0ml @15:48:00.30 Exhaust.select :close @15:48:00.99 SSV.dial CBV @15:48:01.02 CBV.dial SSV @15:48:01.92 Allowing 130s for 25.0ml of remaining 525.0ml @15:48:01.93 Intake.select :open @15:48:02.89 AFV.select :carrierSolution @15:48:03.00 delay 2 seconds @15:48:04.11 Intake @34.8psia, vacuum from 20.0 to 24.0psi @15:48:04.13 SS.reconfigure SSinhale.with maxSpeed: 32 @15:48:04.18 SS.seek 25ml @15:48:05.03 AS.reconfigure ASconfig @15:48:05.08 AS.seek 1ml @15:48:53.09 delay 1 second @15:48:54.10 AFV.select :holdingCoil @15:48:54.21 delay 1 second @15:48:55.23 ASV.dial :wasteD @15:48:56.26 AS.seek 0ml @15:48:59.22 Stroke completed -- waiting for pressure to equalize ... @15:49:04.41 Intake.select :close @15:49:04.57 SSV.dial :exhaust @15:49:05.42 Exhaust.select :open @15:49:05.53 SS.reconfigure SSexhale @15:49:05.58 SS.seek 0ml @15:49:14.85 Exhaust.select :close @15:49:14.97 SSV.dial CBV @15:49:15.00 CBV.dial SSV @15:49:15.89 Allowing 130s for 25.0ml of remaining 500.0ml @15:49:15.90 Intake.select :open @15:49:18.09 Intake @34.4psia, vacuum from 20.0 to 24.0psi @15:49:18.11 SS.reconfigure SSinhale.with maxSpeed: 32 @15:49:18.16 SS.seek 25ml @15:49:54.06 Priming Eluate @15:49:54.07 AFV.select :carrierSolution @15:49:54.18 delay 2 seconds @15:49:56.21 AS.seek 0.5ml @15:50:13.16 Stroke completed -- waiting for pressure to equalize ... @15:50:18.33 Intake.select :close @15:50:18.49 SSV.dial :exhaust @15:50:19.36 Exhaust.select :open @15:50:19.47 SS.reconfigure SSexhale @15:50:19.52 SS.seek 0ml @15:50:25.29 delay 1 second @15:50:26.30 AFV.select :holdingCoil @15:50:26.41 ATV.dial ASV @15:50:26.42 ASV.dial :SPErecovery @15:50:27.00 Swish #1 of 2 @15:50:27.09 AS.reconfigure ASslow0 @15:50:27.14 AS.seek 0.6ml @15:50:28.96 Exhaust.select :close @15:50:29.08 SSV.dial CBV @15:50:29.11 CBV.dial SSV @15:50:30.00 Allowing 130s for 25.0ml of remaining 475.0ml @15:50:30.01 Intake.select :open @15:50:32.20 Intake @34.9psia, vacuum from 20.0 to 24.0psi @15:50:32.22 SS.reconfigure SSinhale.with maxSpeed: 32 @15:50:32.26 SS.seek 25ml @15:50:37.61 AS.seek 0.5ml @15:50:48.00 Swish #2 of 2 @15:50:48.09 AS.seek 0.6ml @15:50:58.58 AS.seek 0.5ml @15:51:08.96 delay 3 seconds @15:51:12.04 AS.reconfigure ASslow1 @15:51:12.09 AS.seek 0.684ml @15:51:26.94 Stroke completed -- waiting for pressure to equalize ... @15:51:32.09 Intake.select :close @15:51:32.25 SSV.dial :exhaust @15:51:33.11 Exhaust.select :open @15:51:33.23 SS.reconfigure SSexhale @15:51:33.29 SS.seek 0ml @15:51:42.30 Exhaust.select :close @15:51:42.42 SSV.dial CBV @15:51:42.45 CBV.dial SSV @15:51:43.33 Allowing 130s for 25.0ml of remaining 450.0ml @15:51:43.34 Intake.select :open @15:51:45.53 Intake @34.8psia, vacuum from 20.0 to 24.0psi @15:51:45.55 SS.reconfigure SSinhale.with maxSpeed: 32 @15:51:45.60 SS.seek 25ml @15:51:49.39 delay 3 seconds @15:51:52.42 Eluate primed @15:51:52.43 ATV.dial :waste2 @15:51:53.03 AS.reconfigure ASnorm @15:51:53.08 AS.seek 0ml @15:52:27.91 SPE.coast @15:52:28.00 ASV.dial :SPErecovery @15:52:28.02 Setting up PCR for Ss -- writing /var/log/esp/Ss18oct2115h300ml.pcr @15:52:28.04 Building PCR Slug from ASV with MM1 mix, PP2 primer @15:52:28.05 Priming with carrier @15:52:28.06 ATV.dial ARV @15:52:29.12 ARV.dial :carrier @15:52:29.49 AS.seek 0.5ml @15:52:41.07 Stroke completed -- waiting for pressure to equalize ... @15:52:46.26 Intake.select :close @15:52:46.43 SSV.dial :exhaust @15:52:47.26 Exhaust.select :open @15:52:47.37 SS.reconfigure SSexhale @15:52:47.42 SS.seek 0ml @15:52:55.08 AFV.select :carrierSolution @15:52:55.20 delay 2 seconds @15:52:56.21 Exhaust.select :close @15:52:56.33 SSV.dial CBV @15:52:56.36 CBV.dial SSV @15:52:57.23 Allowing 130s for 25.0ml of remaining 425.0ml @15:52:57.24 Intake.select :open @15:52:57.29 AS.reconfigure ASconfig @15:52:57.35 AS.seek 1ml @15:52:59.48 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:52:59.50 SS.reconfigure SSinhale.with maxSpeed: 32 @15:52:59.55 SS.seek 25ml @15:53:26.37 delay 1 second @15:53:27.39 AFV.select :holdingCoil @15:53:27.50 delay 1 second @15:53:28.52 ATV.dial :waste @15:53:30.00 AS.reconfigure ASnorm @15:53:30.05 AS.seek :empty @15:53:54.80 Stroke completed -- waiting for pressure to equalize ... @15:54:00.61 Intake.select :close @15:54:01.36 SSV.dial :exhaust @15:54:02.20 Exhaust.select :open @15:54:02.31 SS.reconfigure SSexhale @15:54:02.37 SS.seek 0ml @15:54:11.91 Exhaust.select :close @15:54:12.03 SSV.dial CBV @15:54:12.06 CBV.dial SSV @15:54:12.93 Allowing 130s for 25.0ml of remaining 400.0ml @15:54:12.94 Intake.select :open @15:54:15.13 Intake @34.6psia, vacuum from 20.0 to 24.0psi @15:54:15.15 SS.reconfigure SSinhale.with maxSpeed: 32 @15:54:15.20 SS.seek 25ml @15:54:20.77 AFV.select :carrierSolution @15:54:20.89 delay 2 seconds @15:54:22.92 AS.reconfigure ASconfig @15:54:22.97 AS.seek 0.5ml @15:54:52.09 delay 1 second @15:54:53.10 AFV.select :holdingCoil @15:54:53.21 Building Slug @15:54:53.22 ATV.dial :ARV @15:54:54.81 delay 2 seconds @15:54:56.83 ARV.dial :air1 @15:54:57.23 delay 2 seconds @15:54:59.32 AS.reconfigure ASslow2 @15:54:59.37 AS.seek 0.506ml @15:55:05.76 delay 2 seconds @15:55:07.04 Vacuum too high -- waiting up to 40s @15:55:07.85 ARV.dial :MM1 @15:55:08.44 delay 2 seconds @15:55:08.67 Intake @35.1psia, vacuum from 20.0 to 24.0psi @15:55:08.69 SS.reconfigure SSinhale.with maxSpeed: 29 @15:55:08.74 SS.seek 25ml @15:55:10.46 Adding MM1 @15:55:10.53 AS.seek 0.524ml @15:55:12.32 Stroke completed -- waiting for pressure to equalize ... @15:55:16.83 Intake.select :close @15:55:16.99 SSV.dial :exhaust @15:55:17.84 Exhaust.select :open @15:55:17.95 SS.reconfigure SSexhale @15:55:18.01 SS.seek 0ml @15:55:27.27 Exhaust.select :close @15:55:27.39 SSV.dial CBV @15:55:27.42 CBV.dial SSV @15:55:28.29 Allowing 130s for 25.0ml of remaining 375.0ml @15:55:28.30 Intake.select :open @15:55:29.09 delay 25 seconds @15:55:30.49 Intake @34.7psia, vacuum from 20.0 to 24.0psi @15:55:30.51 SS.reconfigure SSinhale.with maxSpeed: 29 @15:55:30.56 SS.seek 25ml @15:55:54.11 ARV.dial :PP2 @15:55:54.51 Adding PP2 @15:55:54.52 delay 2 seconds @15:55:56.61 AS.seek 0.53ml @15:56:03.20 delay 10 seconds @15:56:13.22 ARV.dial :air2 @15:56:13.61 adding ARV air @15:56:13.63 delay 2 seconds @15:56:15.71 AS.seek 0.536ml @15:56:21.95 delay 5 seconds @15:56:26.97 ARV.dial :carrier @15:56:28.22 delay 7 seconds @15:56:31.30 Stroke completed -- waiting for pressure to equalize ... @15:56:35.30 AS.seek 0.594ml @15:56:36.41 Intake.select :close @15:56:36.58 SSV.dial :exhaust @15:56:37.42 Exhaust.select :open @15:56:37.54 SS.reconfigure SSexhale @15:56:37.60 SS.seek 0ml @15:56:46.70 Exhaust.select :close @15:56:46.81 SSV.dial CBV @15:56:46.84 CBV.dial SSV @15:56:47.70 Allowing 130s for 25.0ml of remaining 350.0ml @15:56:47.71 Intake.select :open @15:56:49.96 Intake @34.3psia, vacuum from 20.0 to 24.0psi @15:56:49.98 SS.reconfigure SSinhale.with maxSpeed: 29 @15:56:50.03 SS.seek 25ml @15:57:34.06 delay 5 seconds @15:57:39.08 ATV.dial :ASV @15:57:39.48 delay 2 seconds @15:57:41.57 AS.seek 0.6ml @15:57:47.90 delay 4 seconds @15:57:50.67 Stroke completed -- waiting for pressure to equalize ... @15:57:51.96 ATV.dial ARV @15:57:52.58 delay 2 seconds @15:57:54.60 ARV.dial :carrier @15:57:54.62 delay 2 seconds @15:57:55.85 Intake.select :close @15:57:56.02 SSV.dial :exhaust @15:57:56.71 AS.seek 0.65ml @15:57:56.86 Exhaust.select :open @15:57:56.98 SS.reconfigure SSexhale @15:57:57.03 SS.seek 0ml @15:58:06.21 Exhaust.select :close @15:58:06.33 SSV.dial CBV @15:58:06.36 CBV.dial SSV @15:58:07.23 Allowing 130s for 25.0ml of remaining 325.0ml @15:58:07.24 Intake.select :open @15:58:09.42 Intake @34.9psia, vacuum from 20.0 to 24.0psi @15:58:09.45 SS.reconfigure SSinhale.with maxSpeed: 29 @15:58:09.49 SS.seek 25ml @15:58:47.45 delay 4 seconds @15:58:51.48 ATV.dial PCR @15:58:52.08 ASV.dial :waste1 @15:58:52.87 ARV.dial :air1 @15:58:53.26 Positioning Slug by 0.324ml @15:58:53.35 AS.seek 0.326ml @15:59:09.85 Stroke completed -- waiting for pressure to equalize ... @15:59:15.00 Intake.select :close @15:59:15.16 SSV.dial :exhaust @15:59:16.00 Exhaust.select :open @15:59:16.11 SS.reconfigure SSexhale @15:59:16.16 SS.seek 0ml @15:59:25.68 Exhaust.select :close @15:59:25.80 SSV.dial CBV @15:59:25.83 CBV.dial SSV @15:59:26.68 Allowing 130s for 25.0ml of remaining 300.0ml @15:59:26.69 Intake.select :open @15:59:28.88 Intake @34.9psia, vacuum from 20.0 to 24.0psi @15:59:28.90 SS.reconfigure SSinhale.with maxSpeed: 29 @15:59:28.95 SS.seek 25ml @16:00:29.35 Stroke completed -- waiting for pressure to equalize ... @16:00:34.52 Intake.select :close @16:00:34.68 SSV.dial :exhaust @16:00:35.53 Exhaust.select :open @16:00:35.64 SS.reconfigure SSexhale @16:00:35.69 SS.seek 0ml @16:00:44.61 Exhaust.select :close @16:00:44.73 SSV.dial CBV @16:00:44.76 CBV.dial SSV @16:00:45.61 Allowing 130s for 25.0ml of remaining 275.0ml @16:00:45.62 Intake.select :open @16:00:47.80 Intake @35.2psia, vacuum from 20.0 to 24.0psi @16:00:47.83 SS.reconfigure SSinhale.with maxSpeed: 29 @16:00:47.87 SS.seek 25ml @16:01:49.13 Stroke completed -- waiting for pressure to equalize ... @16:01:54.91 Intake.select :close @16:01:55.07 SSV.dial :exhaust @16:01:55.90 Exhaust.select :open @16:01:56.01 SS.reconfigure SSexhale @16:01:56.06 SS.seek 0ml @16:02:05.07 Exhaust.select :close @16:02:05.19 SSV.dial CBV @16:02:05.22 CBV.dial SSV @16:02:06.09 Allowing 130s for 25.0ml of remaining 250.0ml @16:02:06.10 Intake.select :open @16:02:08.29 Intake @35.4psia, vacuum from 20.0 to 24.0psi @16:02:08.31 SS.reconfigure SSinhale.with maxSpeed: 29 @16:02:08.35 SS.seek 25ml @16:02:52.45 Vacuum too high -- waiting up to 40s @16:02:53.81 Intake @35.0psia, vacuum from 20.0 to 24.0psi @16:02:53.83 SS.reconfigure SSinhale.with maxSpeed: 26 @16:02:53.88 SS.seek 25ml @16:03:12.67 Stroke completed -- waiting for pressure to equalize ... @16:03:18.42 Intake.select :close @16:03:18.59 SSV.dial :exhaust @16:03:19.43 Exhaust.select :open @16:03:19.55 SS.reconfigure SSexhale @16:03:19.61 SS.seek 0ml @16:03:28.91 Exhaust.select :close @16:03:29.03 SSV.dial CBV @16:03:29.06 CBV.dial SSV @16:03:29.93 Allowing 130s for 25.0ml of remaining 225.0ml @16:03:29.94 Intake.select :open @16:03:32.12 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:03:32.14 SS.reconfigure SSinhale.with maxSpeed: 26 @16:03:32.19 SS.seek 25ml @16:04:19.23 delay 5 seconds @16:04:24.25 ATV.dial ARV @16:04:25.12 ASV.dial :PCRlock @16:04:25.48 delay 5 seconds @16:04:30.51 Running Ss Profile60C350 thermocycling @16:04:30.52 PCR.program Profile60C350 @16:04:30.67 Profile60C350 should complete in < 1:48:05 @16:04:30.68 PCR.run 2:20:06.25 @16:04:39.33 Stroke completed -- waiting for pressure to equalize ... @16:04:44.50 Intake.select :close @16:04:44.67 SSV.dial :exhaust @16:04:45.51 Exhaust.select :open @16:04:45.62 SS.reconfigure SSexhale @16:04:45.67 SS.seek 0ml @16:04:54.98 Exhaust.select :close @16:04:55.09 SSV.dial CBV @16:04:55.12 CBV.dial SSV @16:04:55.98 Allowing 130s for 25.0ml of remaining 200.0ml @16:04:55.99 Intake.select :open @16:04:58.18 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:04:58.20 SS.reconfigure SSinhale.with maxSpeed: 26 @16:04:58.25 SS.seek 25ml @16:06:05.67 Stroke completed -- waiting for pressure to equalize ... @16:06:11.42 Intake.select :close @16:06:11.59 SSV.dial :exhaust @16:06:12.45 Exhaust.select :open @16:06:12.56 SS.reconfigure SSexhale @16:06:12.62 SS.seek 0ml @16:06:22.03 Exhaust.select :close @16:06:22.15 SSV.dial CBV @16:06:22.18 CBV.dial SSV @16:06:23.03 Allowing 130s for 25.0ml of remaining 175.0ml @16:06:23.04 Intake.select :open @16:06:25.22 Intake @34.9psia, vacuum from 20.0 to 24.0psi @16:06:25.24 SS.reconfigure SSinhale.with maxSpeed: 26 @16:06:25.29 SS.seek 25ml @16:07:32.53 Stroke completed -- waiting for pressure to equalize ... @16:07:38.33 Intake.select :close @16:07:38.50 SSV.dial :exhaust @16:07:39.34 Exhaust.select :open @16:07:39.45 SS.reconfigure SSexhale @16:07:39.51 SS.seek 0ml @16:07:48.47 Exhaust.select :close @16:07:48.59 SSV.dial CBV @16:07:48.62 CBV.dial SSV @16:07:49.50 Allowing 130s for 25.0ml of remaining 150.0ml @16:07:49.51 Intake.select :open @16:07:51.70 Intake @35.3psia, vacuum from 20.0 to 24.0psi @16:07:51.72 SS.reconfigure SSinhale.with maxSpeed: 26 @16:07:51.77 SS.seek 25ml @16:08:59.28 Stroke completed -- waiting for pressure to equalize ... @16:09:05.70 Intake.select :close @16:09:05.87 SSV.dial :exhaust @16:09:06.72 Exhaust.select :open @16:09:06.83 SS.reconfigure SSexhale @16:09:06.88 SS.seek 0ml @16:09:16.02 Exhaust.select :close @16:09:16.14 SSV.dial CBV @16:09:16.17 CBV.dial SSV @16:09:17.04 Allowing 130s for 25.0ml of remaining 125.0ml @16:09:17.05 Intake.select :open @16:09:19.24 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:09:19.26 SS.reconfigure SSinhale.with maxSpeed: 26 @16:09:19.31 SS.seek 25ml @16:10:26.69 Stroke completed -- waiting for pressure to equalize ... @16:10:33.03 Intake.select :close @16:10:33.19 SSV.dial :exhaust @16:10:34.03 Exhaust.select :open @16:10:34.15 SS.reconfigure SSexhale @16:10:34.21 SS.seek 0ml @16:10:43.10 Exhaust.select :close @16:10:43.22 SSV.dial CBV @16:10:43.25 CBV.dial SSV @16:10:44.12 Allowing 130s for 25.0ml of remaining 100.0ml @16:10:44.13 Intake.select :open @16:10:46.32 Intake @35.6psia, vacuum from 20.0 to 24.0psi @16:10:46.34 SS.reconfigure SSinhale.with maxSpeed: 26 @16:10:46.39 SS.seek 25ml @16:11:54.11 Stroke completed -- waiting for pressure to equalize ... @16:12:00.55 Intake.select :close @16:12:00.72 SSV.dial :exhaust @16:12:01.56 Exhaust.select :open @16:12:01.67 SS.reconfigure SSexhale @16:12:01.72 SS.seek 0ml @16:12:10.99 Exhaust.select :close @16:12:11.11 SSV.dial CBV @16:12:11.14 CBV.dial SSV @16:12:12.01 Allowing 130s for 25.0ml of remaining 75.0ml @16:12:12.02 Intake.select :open @16:12:14.20 Intake @35.5psia, vacuum from 20.0 to 24.0psi @16:12:14.22 SS.reconfigure SSinhale.with maxSpeed: 26 @16:12:14.27 SS.seek 25ml @16:13:21.66 Stroke completed -- waiting for pressure to equalize ... @16:13:28.08 Intake.select :close @16:13:28.25 SSV.dial :exhaust @16:13:29.09 Exhaust.select :open @16:13:29.20 SS.reconfigure SSexhale @16:13:29.25 SS.seek 0ml @16:13:38.46 Exhaust.select :close @16:13:38.58 SSV.dial CBV @16:13:38.61 CBV.dial SSV @16:13:39.47 Allowing 130s for 25.0ml of remaining 50.0ml @16:13:39.48 Intake.select :open @16:13:42.27 Intake @35.1psia, vacuum from 20.0 to 24.0psi @16:13:42.30 SS.reconfigure SSinhale.with maxSpeed: 26 @16:13:42.35 SS.seek 25ml @16:14:50.11 Stroke completed -- waiting for pressure to equalize ... @16:14:56.52 Intake.select :close @16:14:56.68 SSV.dial :exhaust @16:14:57.53 Exhaust.select :open @16:14:57.64 SS.reconfigure SSexhale @16:14:57.69 SS.seek 0ml @16:15:07.27 Exhaust.select :close @16:15:07.39 SSV.dial CBV @16:15:07.42 CBV.dial SSV @16:15:08.29 Allowing 130s for 25.0ml of remaining 25.0ml @16:15:08.30 Intake.select :open @16:15:10.49 Intake @34.8psia, vacuum from 20.0 to 24.0psi @16:15:10.51 SS.reconfigure SSinhale.with maxSpeed: 26 @16:15:10.56 SS.seek 24.96ml @16:16:17.99 Stroke completed -- waiting for pressure to equalize ... @16:16:25.06 Intake.select :close @16:16:25.22 SSV.dial :exhaust @16:16:26.06 Exhaust.select :open @16:16:26.18 SS.reconfigure SSexhale @16:16:26.23 SS.seek 0ml @16:16:35.52 Exhaust.select :close @16:16:35.64 Sampled 1500.0ml @16:16:35.66 Drying puck before fixing for 4:00 @16:16:35.67 CBV.dial :waste2b @16:16:36.51 delay 1:00 @16:17:36.54 CSV.dial :air @16:17:37.02 CS.reconfigure CSfast @16:17:37.08 CS.seek 10ml @16:18:02.65 Pumping wcr puck from tube 4 up to 55psi... @16:18:02.66 CBV.dial SSV @16:18:04.59 CBV.dial between CSV and waste2b @16:18:05.76 CSV.dial between air and CTV @16:18:06.13 CS.seek 6.899ml @16:18:14.71 CTV.dial CSV,avoiding: :air @16:18:14.74 CSV.dial CTV @16:18:15.62 CS.reconfigure CSpressurize @16:18:15.68 CS.seek 0ml @16:18:33.93 CSV.dial :air @16:18:34.44 CS.reconfigure CSfast @16:18:34.50 CS.seek 10ml @16:19:00.08 CSV.dial between air and CTV @16:19:00.45 CS.seek 2.245ml @16:19:20.49 CTV.dial CSV,avoiding: :air @16:19:20.52 CSV.dial CTV @16:19:20.86 CS.reconfigure CSpressurize @16:19:20.91 CS.seek 0ml @16:19:23.73 CBV.dial :waste2b @16:19:24.09 CS.reconfigure CSconfig @16:19:24.14 CS.seek :empty @16:19:28.52 delay 1:00 @16:20:28.55 CTV.dial :air,avoiding: :intake @16:20:29.99 Fix #1 of 2 (with rnal) for 2:00 @16:20:30.01 CSV.dial CSR @16:20:30.55 Pulling 2ml of CSR.rnal @16:20:30.57 CSR.select :rnal @16:20:30.69 CS.seek 2ml @16:20:36.81 delay 5 seconds @16:20:41.83 CSR.select :air @16:20:41.96 CS.reconfigure CSfast @16:20:42.01 CS.seek 10ml @16:21:02.65 Pumping wcr puck from tube 4 up to 55psi... @16:21:02.66 CBV.dial SSV @16:21:04.05 CBV.dial between CSV and waste2b @16:21:05.13 CSV.dial between air and CTV @16:21:05.80 CS.seek 6.781ml @16:21:14.68 CTV.dial CSV,avoiding: :air @16:21:14.71 CSV.dial CTV @16:21:16.24 CS.reconfigure CSpressurize @16:21:16.30 CS.seek 0ml @16:21:34.20 CSV.dial :air @16:21:35.27 CS.reconfigure CSfast @16:21:35.35 CS.seek 10ml @16:22:00.78 CSV.dial between air and CTV @16:22:01.13 CS.seek 2.02ml @16:22:21.62 CTV.dial CSV,avoiding: :air @16:22:21.65 CSV.dial CTV @16:22:22.00 CS.reconfigure CSpressurize @16:22:22.06 CS.seek 0ml @16:22:23.33 delay 2:00 @16:24:23.35 CBV.dial :waste2b @16:24:23.73 CS.reconfigure CSconfig @16:24:23.78 CS.seek :empty @16:24:29.12 delay 1:00 @16:25:29.14 CTV.dial :air,avoiding: :intake @16:25:30.59 Fix #2 of 2 (with rnal) for 2:00 @16:25:30.61 CSV.dial CSR @16:25:31.15 Pulling 2ml of CSR.rnal @16:25:31.17 CSR.select :rnal @16:25:31.30 CS.seek 2ml @16:25:37.37 delay 5 seconds @16:25:42.39 CSR.select :air @16:25:42.52 CS.reconfigure CSfast @16:25:42.57 CS.seek 10ml @16:26:02.87 Pumping wcr puck from tube 4 up to 55psi... @16:26:02.88 CBV.dial SSV @16:26:04.29 CBV.dial between CSV and waste2b @16:26:05.36 CSV.dial between air and CTV @16:26:06.15 CS.seek 6.761ml @16:26:15.03 CTV.dial CSV,avoiding: :air @16:26:15.06 CSV.dial CTV @16:26:16.56 CS.reconfigure CSpressurize @16:26:16.62 CS.seek 0ml @16:26:34.49 CSV.dial :air @16:26:35.00 CS.reconfigure CSfast @16:26:35.06 CS.seek 10ml @16:27:00.56 CSV.dial between air and CTV @16:27:00.92 CS.seek 2.002ml @16:27:21.41 CTV.dial CSV,avoiding: :air @16:27:21.44 CSV.dial CTV @16:27:21.77 CS.reconfigure CSpressurize @16:27:21.82 CS.seek 0ml @16:27:22.90 delay 2:00 @16:29:22.93 CBV.dial :waste2b @16:29:23.28 CS.reconfigure CSconfig @16:29:23.33 CS.seek :empty @16:29:28.85 delay 1:00 @16:30:28.88 CTV.dial :air,avoiding: :intake @16:30:30.40 Storing wcr puck in Collection Clamp to tube 3 @16:30:30.60 SC.reconfigure SCtight @16:30:30.65 SC.seek 3 @16:30:32.74 SC.reconfigure SCloose @16:30:32.97 Elbow.reconfigure TightElbow @16:30:33.02 Elbow.seek 3 @16:30:34.93 Forearm.reconfigure TightForearm @16:30:34.98 Forearm.seek 3 + 700 counts @16:30:36.07 SE.reconfigure SEpick @16:30:36.15 SE.seek beamBreak @16:30:38.80 SE.configure SEconfig @16:30:38.97 Elbow.reconfigure LimpElbow @16:30:39.03 Forearm.reconfigure LooseForearm @16:30:39.08 Forearm.seek 3 @16:30:39.36 Elbow.hold @16:30:39.44 Elbow.reconfigure TightElbow @16:30:39.55 SE.seek 5.08 Pucks below Top Plate @16:30:40.03 Forearm.force -20 @16:30:40.08 Forearm.seek 3 + 348 counts @16:30:40.43 Forearm.reconfigure TightForearm @16:30:40.48 Forearm.seek 3 + 700 counts @16:30:40.90 SE.seek 6.08 Pucks below Top Plate @16:30:41.60 Forearm.reconfigure LooseForearm @16:30:41.66 Forearm.seek :retract @16:30:42.60 Elbow.seek CC @16:30:44.35 Elbow.reconfigure LooseElbow @16:30:44.47 Forearm.seek CC @16:30:45.45 Hand.close @16:30:46.16 CC.seek :open @16:30:51.30 Forearm.seek :retract @16:30:52.27 Elbow.reconfigure TightElbow @16:30:52.32 Elbow.seek 3 @16:30:54.17 Elbow.reconfigure LimpElbow @16:30:54.23 Forearm.seek 3 @16:30:55.25 Elbow.hold @16:30:55.33 Elbow.reconfigure TightElbow @16:30:55.39 Hand.open @16:30:56.30 Forearm.force -20 @16:30:56.35 Forearm.seek 3 + 339 counts @16:30:56.70 Forearm.reconfigure TightForearm @16:30:56.75 Forearm.seek 3 + 700 counts @16:30:57.16 SE.seek :down @16:30:58.35 Forearm.reconfigure LooseForearm @16:30:58.40 Forearm.seek :retract @16:30:59.49 SE.coast @16:30:59.53 SC.coast @16:30:59.58 Starting finalPurge @16:30:59.59 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:30:59.67 Hand.open @16:31:00.05 Elbow.seek FlushPuck::Garage @16:31:02.12 Elbow.reconfigure LooseElbow @16:31:02.22 Forearm.seek :garage @16:31:03.21 Hand.close @16:31:03.93 Forearm.seek :retract @16:31:04.91 Elbow.reconfigure TightElbow @16:31:04.96 Elbow.seek CC @16:31:07.80 Elbow.reconfigure LooseElbow @16:31:07.90 Forearm.reconfigure TightForearm @16:31:07.95 Forearm.seek CC @16:31:09.24 CC.seek :closed @16:31:13.48 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.20 @16:31:13.58 Hand.open @16:31:14.36 Forearm.reconfigure LooseForearm @16:31:14.41 Forearm.seek :retract @16:31:15.49 Flushing the Collection Valve::Manifold @16:31:15.65 CSV.dial CSR @16:31:16.21 Pulling 5ml of CSR.flush @16:31:16.22 CSR.select :flush @16:31:16.34 CS.seek 5ml @16:31:30.22 delay 5 seconds @16:31:35.25 CSR.select :air @16:31:35.43 CS.reconfigure CSfast @16:31:35.48 CS.seek 7.501ml @16:31:42.52 CSV.dial :waste @16:31:44.07 CS.reconfigure CSconfig @16:31:44.12 CS.seek 0ml @16:32:04.19 Flushing lower manifold's waste1 port @16:32:04.33 CSV.dial :flush @16:32:04.85 CS.reconfigure CSfast @16:32:04.90 CS.seek 5ml @16:32:18.07 delay 5 seconds @16:32:23.08 CSV.dial :air @16:32:23.65 CS.seek 7ml @16:32:29.60 CSV.dial CTV @16:32:29.63
CTV.dial CSV @16:32:31.13 CBV.dial :waste1 @16:32:32.00 CS.seek 0ml @16:32:49.82 Flushing lower manifold's waste2b port @16:32:49.96 CSV.dial :flush @16:32:50.78 CS.seek 5ml @16:33:03.88 delay 5 seconds @16:33:08.89 CSV.dial :air @16:33:09.51 CS.seek 7ml @16:33:15.32 CSV.dial CTV @16:33:15.35
CTV.dial CSV @16:33:15.85 CBV.dial :waste2b @16:33:16.69 CS.seek 0ml @16:33:34.54 Flushing upper manifold @16:33:34.68 CSV.dial :flush @16:33:35.49 CS.seek 5ml @16:33:48.60 delay 5 seconds @16:33:53.61 CSV.dial :air @16:33:54.20 CS.seek 7ml @16:34:00.04 CSV.dial CBV @16:34:00.07
CBV.dial CSV @16:34:01.26 CTV.dial :air @16:34:02.71 CS.seek 0ml @16:34:20.57 delay 7 seconds @16:34:27.60 CS.seek 3ml @16:34:35.90 CSV.dial :waste @16:34:36.40 CS.seek 0ml @16:34:44.72 CSV.dial :flush @16:34:45.22 CS.seek 3ml @16:34:53.47 delay 5 seconds @16:34:58.48 CSV.dial :air @16:34:59.04 CS.seek 5ml @16:35:04.81 CSV.dial :air @16:35:04.84 CS.seek 0ml @16:35:17.85 delay 7 seconds @16:35:24.87 CS.seek 3ml @16:35:33.12 CSV.dial :waste @16:35:33.94 CS.seek 0ml @16:35:42.23 Disinfecting external Intake with mfbkill @16:35:42.37 CSV.dial CSR @16:35:43.87 Pulling 8ml of CSR.mfbkill @16:35:43.88 CSR.select :mfbkill @16:35:44.00 CS.seek 8ml @16:36:04.48 delay 5 seconds @16:36:09.50 CSR.select :air @16:36:09.64 CS.seek 10ml @16:36:15.48 CSV.dial CBV @16:36:15.51
CBV.dial CSV @16:36:16.64 CTV.dial :intake @16:36:17.49 Intake.select :open @16:36:17.61 CS.seek 2ml @16:36:38.68 Intake.select :close @16:36:38.80 CTV.dial :air @16:36:39.62 CS.reconfigure CSconfig @16:36:39.67 CS.seek 0ml @16:36:45.85 Flushing lower manifold's waste1 port @16:36:45.86 CSV.dial CSR @16:36:47.06 Pulling 4ml of CSR.mfbkill @16:36:47.07 CSR.select :mfbkill @16:36:47.20 CS.seek 4ml @16:36:58.35 delay 5 seconds @16:37:03.36 CSR.select :air @16:37:03.49 CS.reconfigure CSfast @16:37:03.54 CS.seek 10ml @16:37:18.91 CSV.dial CTV @16:37:18.94
CTV.dial CSV @16:37:20.48 CBV.dial :waste1 @16:37:20.99 CS.seek 0ml @16:37:46.13 Flushing lower manifold's waste2b port @16:37:46.14 CSV.dial CSR @16:37:46.71 Pulling 4ml of CSR.mfbkill @16:37:46.72 CSR.select :mfbkill @16:37:46.85 CS.reconfigure CSconfig @16:37:46.89 CS.seek 4ml @16:37:58.13 delay 5 seconds @16:38:03.14 CSR.select :air @16:38:03.27 CS.reconfigure CSfast @16:38:03.32 CS.seek 10ml @16:38:18.76 CSV.dial CTV @16:38:18.79
CTV.dial CSV @16:38:19.31 CBV.dial :waste2b @16:38:20.16 CS.seek 0ml @16:38:45.20 Killing upper manifold @16:38:45.21 CSV.dial CSR @16:38:45.77 Pulling 4ml of CSR.mfbkill @16:38:45.78 CSR.select :mfbkill @16:38:45.91 CS.reconfigure CSconfig @16:38:45.95 CS.seek 4ml @16:38:57.35 delay 5 seconds @16:39:02.37 CSR.select :air @16:39:02.50 CS.reconfigure CSfast @16:39:02.55 CS.seek 10ml @16:39:17.98 CSV.dial CBV @16:39:18.01
CBV.dial CSV @16:39:19.18 CTV.dial :air @16:39:21.24 CS.seek 0ml @16:39:46.29 delay 7 seconds @16:39:53.31 CS.seek 3ml @16:40:01.67 CSV.dial :waste @16:40:02.16 CS.seek 0ml @16:40:10.29 Flushing upper manifold @16:40:10.43 CSV.dial :flush @16:40:10.94 CS.seek 5ml @16:40:23.92 delay 5 seconds @16:40:28.93 CSV.dial :air @16:40:29.50 CS.seek 7ml @16:40:35.42 CSV.dial CBV @16:40:35.45
CBV.dial CSV @16:40:36.59 CTV.dial :air @16:40:36.61 CS.seek 0ml @16:40:54.42 delay 7 seconds @16:41:01.45 CS.seek 3ml @16:41:09.66 CSV.dial :waste @16:41:10.19 CS.seek 0ml @16:41:18.60 CSV.dial :flush @16:41:19.10 CS.seek 3ml @16:41:27.39 delay 5 seconds @16:41:32.41 CSV.dial :air @16:41:33.10 CS.seek 5ml @16:41:39.03 CSV.dial :air @16:41:39.06 CS.seek 0ml @16:41:52.11 delay 7 seconds @16:41:59.13 CS.seek 3ml @16:42:07.28 CSV.dial :waste @16:42:08.07 CS.seek 0ml @16:42:16.32 Flushing lower manifold's waste1 port @16:42:16.46 CSV.dial :flush @16:42:16.96 CS.seek 5ml @16:42:29.98 delay 5 seconds @16:42:34.99 CSV.dial :air @16:42:35.58 CS.seek 7ml @16:42:41.36 CSV.dial CTV @16:42:41.39
CTV.dial CSV @16:42:42.89 CBV.dial :waste1 @16:42:43.41 CS.seek 0ml @16:43:01.14 Flushing lower manifold's waste2b port @16:43:01.28 CSV.dial :flush @16:43:02.10 CS.seek 5ml @16:43:15.11 delay 5 seconds @16:43:20.12 CSV.dial :air @16:43:20.71 CS.seek 7ml @16:43:26.70 CSV.dial CTV @16:43:26.73
CTV.dial CSV @16:43:27.23 CBV.dial :waste2b @16:43:28.09 CS.seek 0ml @16:43:46.01 Disinfecting external Intake with kill @16:43:46.78 CSV.dial CSR @16:43:47.33 Pulling 8ml of CSR.kill CSR.select :kill @16:43:47.46 CS.seek 8ml @16:44:07.80 delay 5 seconds @16:44:12.82 CSR.select :air @16:44:12.96 CS.seek 10ml @16:44:18.80 CSV.dial CBV @16:44:18.83
CBV.dial CSV @16:44:20.00 CTV.dial :intake @16:44:20.81 Intake.select :open @16:44:20.93 CS.seek 2ml @16:44:42.44 Intake.select :close @16:44:42.56 CTV.dial :air @16:44:43.40 CS.reconfigure CSconfig @16:44:43.45 CS.seek 0ml @16:44:49.79 CSV.dial CSR @16:44:50.96 Pulling 5ml of CSR.flush @16:44:50.97 CSR.select :flush @16:44:51.10 CS.seek 5ml @16:45:04.83 delay 5 seconds @16:45:09.85 CSR.select :air @16:45:09.99 CS.seek 10ml @16:45:23.94 CSV.dial CBV @16:45:25.07 CS.seek 0ml @16:45:51.58 Garaging FlushPuck in Collection Clamp @16:45:51.66 Elbow.reconfigure TightElbow @16:45:51.71 Elbow.seek CC @16:45:52.03 Elbow.reconfigure LooseElbow @16:45:52.14 Forearm.seek CC @16:45:53.12 Hand.close @16:45:53.93 CC.seek :open @16:45:58.33 Forearm.seek :retract @16:45:59.32 Elbow.reconfigure TightElbow @16:45:59.37 Elbow.seek :garage @16:46:02.59 Elbow.reconfigure LooseElbow @16:46:02.70 Forearm.reconfigure TightForearm @16:46:02.75 Forearm.seek :garage @16:46:03.92 Hand.open @16:46:04.75 Forearm.reconfigure LooseForearm @16:46:04.80 Forearm.seek :retract @16:46:05.84 Starting initialPurge @16:46:05.85 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:46:05.93 Hand.open @16:46:06.25 Elbow.reconfigure TightElbow @16:46:06.30 Elbow.seek FlushPuck::Garage @16:46:06.51 Elbow.reconfigure LooseElbow @16:46:06.62 Forearm.seek :garage @16:46:07.65 Hand.close @16:46:08.44 Forearm.seek :retract @16:46:09.48 Elbow.reconfigure TightElbow @16:46:09.53 Elbow.seek CC @16:46:12.53 Elbow.reconfigure LooseElbow @16:46:12.63 Forearm.reconfigure TightForearm @16:46:12.68 Forearm.seek CC @16:46:14.58 CC.seek :closed @16:46:18.87 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.23 @16:46:18.95 Hand.open @16:46:19.63 Forearm.reconfigure LooseForearm @16:46:19.68 Forearm.seek :retract @16:46:20.67 Flushing the Collection Valve::Manifold @16:46:20.81 CSV.dial CSR @16:46:22.00 Pulling 5ml of CSR.flush @16:46:22.01 CSR.select :flush @16:46:22.14 CS.seek 5ml @16:46:36.02 delay 5 seconds @16:46:41.03 CSR.select :air @16:46:41.22 CS.reconfigure CSfast @16:46:41.27 CS.seek 7.501ml @16:46:48.54 CSV.dial :waste @16:46:49.99 CS.reconfigure CSconfig @16:46:50.04 CS.seek 0ml @16:47:10.14 Purging external Intake @16:47:10.27 CSV.dial :flush @16:47:10.78 CS.reconfigure CSfast @16:47:10.83 CS.seek 10ml @16:47:35.92 delay 5 seconds @16:47:40.94 CSV.dial CBV @16:47:40.97
CBV.dial CSV @16:47:41.78 CTV.dial :intake @16:47:42.59 Intake.select :open @16:47:42.71 CS.seek 0ml @16:48:08.85 Intake.select :close @16:48:08.97 CTV.dial :air @16:48:09.80 Flushing upper manifold @16:48:09.94 CSV.dial :flush @16:48:10.75 CS.seek 5ml @16:48:23.64 delay 5 seconds @16:48:28.65 CSV.dial :air @16:48:29.21 CS.seek 7ml @16:48:35.08 CSV.dial CBV @16:48:35.10
CBV.dial CSV @16:48:36.24 CTV.dial :air @16:48:36.26 CS.seek 0ml @16:48:54.17 delay 7 seconds @16:49:01.19 CS.seek 3ml @16:49:09.41 CSV.dial :waste @16:49:10.03 CS.seek 0ml @16:49:18.33 CSV.dial :flush @16:49:18.86 CS.seek 3ml @16:49:27.05 delay 5 seconds @16:49:32.06 CSV.dial :air @16:49:32.62 CS.seek 5ml @16:49:38.41 CSV.dial :air @16:49:38.44 CS.seek 0ml @16:49:51.49 delay 7 seconds @16:49:58.51 CS.seek 3ml @16:50:06.70 CSV.dial :waste @16:50:08.14 CS.seek 0ml @16:50:16.39 Garaging FlushPuck in Collection Clamp @16:50:16.47 Elbow.reconfigure TightElbow @16:50:16.52 Elbow.seek CC @16:50:16.79 Elbow.reconfigure LooseElbow @16:50:16.89 Forearm.seek CC @16:50:17.88 Hand.close @16:50:18.69 CC.seek :open @16:50:23.09 Forearm.seek :retract @16:50:24.08 Elbow.reconfigure TightElbow @16:50:24.13 Elbow.seek :garage @16:50:27.26 Elbow.reconfigure LooseElbow @16:50:27.37 Forearm.reconfigure TightForearm @16:50:27.42 Forearm.seek :garage @16:50:28.59 Hand.open @16:50:29.38 Forearm.reconfigure LooseForearm @16:50:29.43 Forearm.seek :retract @16:50:30.43 Completed [mbaPhase] begun 14:00:53 @16:50:30.45 Preparing to sleep until Tue Oct 23 14:00:53 +0200 2018 @16:50:30.51 deferred until MFBPCR finish @16:50:30.53
Waiting for MFBPCR til 14:00CEST23-Oct-18 @16:50:30.55 Continuing at 14:00CEST23-Oct-18 @16:50:30.61 GoodNews.email "Then, in tube 4: mbaPhase\n/home/esp/esp2/mission/18tundeployv4.rb:127\n{1 => 22, 2 => 22, 3 => 7, 4 => 15}\nCan@16:50:30, 14.6C, 71% humidity, 14.3psia, 12.625V, 0.846A, 0.846A avg, 10.69W",Subject: "Waiting until 14:00CEST23-Oct-18" @17:27:11.22 PCR.off @17:27:13.26 PCR for Ss completed -- closed /var/log/esp/Ss18oct2115h300ml.pcr @17:27:13.33 Cleaning PCR @17:27:13.34 AFV.select :carrierSolution @17:27:13.45 delay 2 seconds @17:27:15.48 AS.reconfigure ASconfig @17:27:15.53 AS.seek 0.5ml @17:27:25.85 delay 1 second @17:27:26.86 AFV.select :holdingCoil @17:27:26.97 ASV.dial :waste1 @17:27:27.35 ATV.dial :air3 @17:27:28.58 AS.reconfigure ASslow1 @17:27:28.64 AS.seek 0.51ml @17:27:30.91 delay 2 seconds @17:27:32.93 ATV.dial :bleach @17:27:33.55 AS.seek 0.81ml @17:28:34.13 delay 3 seconds @17:28:37.15 ATV.dial :air3 @17:28:37.70 delay 3 seconds @17:28:40.79 AS.seek 0.835ml @17:28:46.07 delay 3 seconds @17:28:49.09 ATV.dial :bleach @17:28:49.70 AS.seek 0.82ml @17:28:53.01 delay 3 seconds @17:28:56.03 ARV.dial :waste @17:28:56.62 delay 3 seconds @17:28:59.64 ATV.dial :ARV @17:29:00.49 AS.reconfigure ASconfig @17:29:00.54 AS.seek 0.47ml @17:29:20.94 AFV.select :carrierSolution @17:29:21.06 delay 2 seconds @17:29:23.09 AS.seek 1ml @17:29:53.84 delay 1 second @17:29:54.85 AFV.select :holdingCoil @17:29:54.96 delay 1 second @17:29:55.99 AS.reconfigure ASfast @17:29:56.04 AS.seek 0ml @17:30:23.37 AFV.select :carrierSolution @17:30:23.49 delay 2 seconds @17:30:25.52 AS.reconfigure ASconfig @17:30:25.57 AS.seek 1ml @17:31:23.59 delay 1 second @17:31:24.75 AFV.select :holdingCoil @17:31:25.28 delay 1 second @17:31:29.72 AS.reconfigure ASfast @17:31:30.08 AS.seek 0ml @17:31:57.42 AFV.select :carrierSolution @17:31:57.54 delay 2 seconds @17:31:59.57 AS.reconfigure ASconfig @17:31:59.62 AS.seek 1ml @17:32:57.37 delay 1 second @17:32:58.38 AFV.select :holdingCoil @17:32:58.49 delay 1 second @17:32:59.52 AS.reconfigure ASfast @17:32:59.57 AS.seek 0ml @17:33:26.92 ARV.dial between carrier and air1 @17:33:27.40 ATV.dial :air3 @17:33:28.64 AS.reconfigure ASslow1 @17:33:28.69 AS.seek 0.01ml @17:33:31.10 delay 2 seconds @17:33:33.11 ATV.dial :bleach @17:33:33.72 AS.seek 0.81ml @17:36:14.87 delay 3 seconds @17:36:17.89 ATV.dial :air3 @17:36:18.43 delay 3 seconds @17:36:21.52 AS.seek 0.835ml @17:36:26.79 delay 3 seconds @17:36:29.81 ATV.dial :bleach @17:36:30.42 AS.seek 0.82ml @17:36:33.73 delay 3 seconds @17:36:36.75 ATV.dial PCR @17:36:37.25 AS.reconfigure ASconfig @17:36:37.30 AS.seek :empty @17:37:24.77 PCR.program :bleachProfile @17:37:24.84 PCR program should complete in < 2:45 @17:37:24.85 PCR.run 10:00 @17:40:35.76 PCR.off @17:40:35.78 AFV.select :carrierSolution @17:40:35.89 delay 2 seconds @17:40:37.92 AS.seek 1ml @17:41:35.76 delay 1 second @17:41:36.77 AFV.select :holdingCoil @17:41:36.88 delay 1 second @17:41:37.91 AS.reconfigure ASfast @17:41:37.96 AS.seek 0ml @17:42:05.31 delay 3 seconds @17:42:08.32 AFV.select :carrierSolution @17:42:08.43 delay 2 seconds @17:42:10.46 AS.reconfigure ASconfig @17:42:10.51 AS.seek 1ml @17:43:08.28 delay 1 second @17:43:09.29 AFV.select :holdingCoil @17:43:09.40 delay 1 second @17:43:10.43 AS.reconfigure ASfast @17:43:10.48 AS.seek 0ml @17:43:37.83 delay 3 seconds @17:43:40.85 AFV.select :carrierSolution @17:43:40.96 delay 2 seconds @17:43:42.99 AS.reconfigure ASconfig @17:43:43.04 AS.seek 1ml @17:44:40.84 delay 1 second @17:44:41.86 AFV.select :holdingCoil @17:44:41.97 delay 1 second @17:44:43.00 AS.reconfigure ASfast @17:44:43.05 AS.seek 0ml @17:45:10.39 delay 3 seconds @17:45:13.41 AFV.select :carrierSolution @17:45:13.52 delay 2 seconds @17:45:15.55 AS.reconfigure ASconfig @17:45:15.60 AS.seek 1ml @17:46:13.39 delay 1 second @17:46:14.40 AFV.select :holdingCoil @17:46:14.51 delay 1 second @17:46:15.54 AS.reconfigure ASfast @17:46:15.59 AS.seek 0ml @17:46:42.95 delay 3 seconds @17:46:45.97 AFV.select :carrierSolution @17:46:46.08 delay 2 seconds @17:46:48.11 AS.reconfigure ASconfig @17:46:48.16 AS.seek 1ml @17:47:45.97 delay 1 second @17:47:46.98 AFV.select :holdingCoil @17:47:47.09 delay 1 second @17:47:48.12 AS.reconfigure ASnorm @17:47:48.17 AS.seek 0ml @17:48:38.92 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118oct2117h300ml.pcr @17:48:38.94 Building PCR Slug from ASV with MM2 mix, PP4 primer @17:48:38.95 Priming with carrier @17:48:38.96 ATV.dial ARV @17:48:39.56 ARV.dial :carrier @17:48:39.91 AS.seek 0.5ml @17:49:05.43 AFV.select :carrierSolution @17:49:05.55 delay 2 seconds @17:49:07.58 AS.reconfigure ASconfig @17:49:07.63 AS.seek 1ml @17:49:36.64 delay 1 second @17:49:37.65 AFV.select :holdingCoil @17:49:37.76 delay 1 second @17:49:38.78 ATV.dial :waste @17:49:40.36 AS.reconfigure ASnorm @17:49:41.01 AS.seek :empty @17:50:31.74 AFV.select :carrierSolution @17:50:31.86 delay 2 seconds @17:50:33.89 AS.reconfigure ASconfig @17:50:33.94 AS.seek 0.5ml @17:51:03.05 delay 1 second @17:51:04.07 AFV.select :holdingCoil @17:51:04.18 Building Slug @17:51:04.19 ATV.dial :ARV @17:51:05.73 delay 2 seconds @17:51:07.75 ARV.dial :air1 @17:51:08.15 delay 2 seconds @17:51:10.24 AS.reconfigure ASslow2 @17:51:10.29 AS.seek 0.506ml @17:51:16.67 delay 2 seconds @17:51:18.69 ARV.dial :MM2 @17:51:20.11 delay 2 seconds @17:51:22.14 Adding MM2 @17:51:22.21 AS.seek 0.524ml @17:51:40.67 delay 25 seconds @17:52:05.69 ARV.dial :PP4 @17:52:06.08 Adding PP4 @17:52:06.09 delay 2 seconds @17:52:08.18 AS.seek 0.53ml @17:52:14.66 delay 10 seconds @17:52:24.68 ARV.dial :air3 @17:52:25.08 adding ARV air @17:52:25.09 delay 2 seconds @17:52:27.18 AS.seek 0.536ml @17:52:33.44 delay 5 seconds @17:52:38.46 ARV.dial :carrier @17:52:39.69 delay 7 seconds @17:52:46.79 AS.seek 0.594ml @17:53:45.27 delay 5 seconds @17:53:50.29 ATV.dial :ASV @17:53:50.69 delay 2 seconds @17:53:52.79 AS.seek 0.6ml @17:53:59.05 delay 4 seconds @17:54:03.07 ATV.dial ARV @17:54:03.61 delay 2 seconds @17:54:05.63 ARV.dial :carrier @17:54:05.65 delay 2 seconds @17:54:07.74 AS.seek 0.65ml @17:54:58.30 delay 4 seconds @17:55:02.32 ATV.dial PCR @17:55:02.92 ASV.dial :waste1 @17:55:02.93 ARV.dial :air1 @17:55:03.32 Positioning Slug by 0.324ml @17:55:03.41 AS.seek 0.326ml @18:00:29.39 delay 5 seconds @18:00:34.41 ATV.dial ARV @18:00:35.16 ASV.dial :PCRlock @18:00:35.55 delay 5 seconds @18:00:40.58 Running Tt1 Profile60C300 thermocycling @18:00:40.59 PCR.program Profile60C300 @18:00:40.73 Profile60C300 should complete in < 1:45:21 @18:00:40.74 PCR.run 2:16:41.25 @19:22:34.23 PCR.off @19:22:35.80 PCR for Tt1 completed -- closed /var/log/esp/Tt118oct2117h300ml.pcr @19:22:35.88 Cleaning PCR @19:22:35.89 AFV.select :carrierSolution @19:22:36.00 delay 2 seconds @19:22:38.03 AS.reconfigure ASconfig @19:22:38.08 AS.seek 0.5ml @19:22:48.39 delay 1 second @19:22:49.40 AFV.select :holdingCoil @19:22:49.51 ASV.dial :waste1 @19:22:49.89 ATV.dial :air3 @19:22:51.13 AS.reconfigure ASslow1 @19:22:51.18 AS.seek 0.51ml @19:22:53.48 delay 2 seconds @19:22:55.50 ATV.dial :bleach @19:22:56.12 AS.seek 0.81ml @19:23:56.73 delay 3 seconds @19:23:59.75 ATV.dial :air3 @19:24:00.30 delay 3 seconds @19:24:03.39 AS.seek 0.835ml @19:24:08.70 delay 3 seconds @19:24:11.72 ATV.dial :bleach @19:24:12.34 AS.seek 0.82ml @19:24:15.66 delay 3 seconds @19:24:18.68 ARV.dial :waste @19:24:19.30 delay 3 seconds @19:24:22.32 ATV.dial :ARV @19:24:23.17 AS.reconfigure ASconfig @19:24:23.22 AS.seek 0.47ml @19:24:43.62 AFV.select :carrierSolution @19:24:43.75 delay 2 seconds @19:24:46.37 AS.seek 1ml @19:25:17.11 delay 1 second @19:25:18.12 AFV.select :holdingCoil @19:25:18.23 delay 1 second @19:25:19.26 AS.reconfigure ASfast @19:25:19.32 AS.seek 0ml @19:25:46.65 AFV.select :carrierSolution @19:25:46.77 delay 2 seconds @19:25:48.80 AS.reconfigure ASconfig @19:25:48.85 AS.seek 1ml @19:26:46.61 delay 1 second @19:26:47.62 AFV.select :holdingCoil @19:26:47.73 delay 1 second @19:26:48.76 AS.reconfigure ASfast @19:26:48.81 AS.seek 0ml @19:27:16.15 AFV.select :carrierSolution @19:27:16.27 delay 2 seconds @19:27:18.30 AS.reconfigure ASconfig @19:27:18.35 AS.seek 1ml @19:28:16.11 delay 1 second @19:28:17.12 AFV.select :holdingCoil @19:28:17.23 delay 1 second @19:28:18.26 AS.reconfigure ASfast @19:28:18.31 AS.seek 0ml @19:28:45.65 ARV.dial between carrier and air1 @19:28:46.16 ATV.dial :air3 @19:28:47.40 AS.reconfigure ASslow1 @19:28:47.45 AS.seek 0.01ml @19:28:49.81 delay 2 seconds @19:28:51.82 ATV.dial :bleach @19:28:52.43 AS.seek 0.81ml @19:31:33.56 delay 3 seconds @19:31:36.58 ATV.dial :air3 @19:31:37.13 delay 3 seconds @19:31:40.22 AS.seek 0.835ml @19:31:45.53 delay 3 seconds @19:31:48.55 ATV.dial :bleach @19:31:49.17 AS.seek 0.82ml @19:31:52.49 delay 3 seconds @19:31:55.51 ATV.dial PCR @19:31:55.91 AS.reconfigure ASconfig @19:31:55.96 AS.seek :empty @19:32:43.41 PCR.program :bleachProfile @19:32:43.48 PCR program should complete in < 2:45 @19:32:43.49 PCR.run 10:00 @19:35:54.40 PCR.off @19:35:54.43 AFV.select :carrierSolution @19:35:54.54 delay 2 seconds @19:35:56.57 AS.seek 1ml @19:36:54.41 delay 1 second @19:36:55.42 AFV.select :holdingCoil @19:36:55.53 delay 1 second @19:36:56.56 AS.reconfigure ASfast @19:36:56.61 AS.seek 0ml @19:37:23.97 delay 3 seconds @19:37:26.99 AFV.select :carrierSolution @19:37:27.10 delay 2 seconds @19:37:29.13 AS.reconfigure ASconfig @19:37:29.19 AS.seek 1ml @19:38:26.94 delay 1 second @19:38:27.95 AFV.select :holdingCoil @19:38:28.06 delay 1 second @19:38:29.09 AS.reconfigure ASfast @19:38:29.14 AS.seek 0ml @19:38:56.51 delay 3 seconds @19:38:59.53 AFV.select :carrierSolution @19:38:59.64 delay 2 seconds @19:39:01.67 AS.reconfigure ASconfig @19:39:01.72 AS.seek 1ml @19:39:59.52 delay 1 second @19:40:00.53 AFV.select :holdingCoil @19:40:00.64 delay 1 second @19:40:01.67 AS.reconfigure ASfast @19:40:01.72 AS.seek 0ml @19:40:29.07 delay 3 seconds @19:40:32.09 AFV.select :carrierSolution @19:40:32.20 delay 2 seconds @19:40:34.23 AS.reconfigure ASconfig @19:40:34.29 AS.seek 1ml @19:41:32.07 delay 1 second @19:41:33.08 AFV.select :holdingCoil @19:41:33.19 delay 1 second @19:41:34.22 AS.reconfigure ASfast @19:41:34.27 AS.seek 0ml @19:42:01.63 delay 3 seconds @19:42:04.65 AFV.select :carrierSolution @19:42:04.76 delay 2 seconds @19:42:06.79 AS.reconfigure ASconfig @19:42:06.84 AS.seek 1ml @19:43:04.63 delay 1 second @19:43:05.64 AFV.select :holdingCoil @19:43:05.75 delay 1 second @19:43:06.78 AS.reconfigure ASnorm @19:43:06.83 AS.seek 0ml @19:43:57.55 Setting up PCR for IPc -- writing /var/log/esp/IPc18oct2119h300ml.pcr @19:43:57.57 Building PCR Slug from ASV with MM1 mix, PP1 primer @19:43:57.59 Priming with carrier ATV.dial ARV @19:43:58.18 ARV.dial :carrier @19:43:59.15 AS.seek 0.5ml @19:44:24.70 AFV.select :carrierSolution @19:44:24.82 delay 2 seconds @19:44:26.85 AS.reconfigure ASconfig @19:44:26.90 AS.seek 1ml @19:44:55.93 delay 1 second @19:44:56.95 AFV.select :holdingCoil @19:44:57.06 delay 1 second @19:44:58.08 ATV.dial :waste @19:44:59.66 AS.reconfigure ASnorm @19:44:59.71 AS.seek :empty @19:45:50.47 AFV.select :carrierSolution @19:45:50.59 delay 2 seconds @19:45:52.62 AS.reconfigure ASconfig @19:45:52.67 AS.seek 0.5ml @19:46:21.74 delay 1 second @19:46:22.75 AFV.select :holdingCoil @19:46:22.86 Building Slug @19:46:22.87 ATV.dial :ARV @19:46:24.41 delay 2 seconds @19:46:26.43 ARV.dial :air1 @19:46:26.83 delay 2 seconds @19:46:28.92 AS.reconfigure ASslow2 @19:46:28.97 AS.seek 0.506ml @19:46:35.24 delay 2 seconds @19:46:37.26 ARV.dial :MM1 @19:46:37.84 delay 2 seconds @19:46:39.87 Adding MM1 @19:46:39.94 AS.seek 0.524ml @19:46:58.27 delay 25 seconds @19:47:23.29 ARV.dial :PP1 @19:47:23.68 Adding PP1 @19:47:23.70 delay 2 seconds @19:47:25.78 AS.seek 0.53ml @19:47:32.13 delay 10 seconds @19:47:42.15 ARV.dial :air1 @19:47:42.52 adding ARV air @19:47:42.54 delay 2 seconds @19:47:44.62 AS.seek 0.536ml @19:47:51.09 delay 5 seconds @19:47:56.11 ARV.dial :carrier @19:47:56.51 delay 7 seconds @19:48:03.60 AS.seek 0.594ml @19:49:02.35 delay 5 seconds @19:49:07.37 ATV.dial :ASV @19:49:07.77 delay 2 seconds @19:49:09.86 AS.seek 0.6ml @19:49:16.48 delay 4 seconds @19:49:20.50 ATV.dial ARV @19:49:21.06 delay 2 seconds @19:49:23.08 ARV.dial :carrier @19:49:23.10 delay 2 seconds @19:49:25.19 AS.seek 0.65ml @19:50:15.81 delay 4 seconds @19:50:19.83 ATV.dial PCR @19:50:20.43 ASV.dial :waste1 @19:50:20.44 ARV.dial :air1 @19:50:20.83 Positioning Slug by 0.324ml @19:50:20.92 AS.seek 0.326ml @19:55:47.01 delay 5 seconds @19:55:52.03 ATV.dial ARV @19:55:52.78 ASV.dial :PCRlock @19:55:53.17 delay 5 seconds @19:55:58.20 Running IPc ProfileIPc thermocycling @19:55:58.21 PCR.program ProfileIPc @19:55:58.37 ProfileIPc should complete in < 1:50:49 @19:55:58.38 PCR.run 2:23:31.25 @21:20:09.42 PCR.off @21:20:12.57 PCR for IPc completed -- closed /var/log/esp/IPc18oct2119h300ml.pcr @21:20:12.58 Full MFB Decontamination @21:20:12.60 Decon extraction column and decon AIV @21:20:12.62 SPE.hold 2:46:40,55C @21:20:12.66 ASV.dial :SPEdelivery @21:20:13.30 delay 20 seconds @21:20:33.32 AFV.select :carrierSolution @21:20:33.43 delay 2 seconds @21:20:35.46 AS.reconfigure ASconfig @21:20:35.51 AS.seek 0.5ml @21:20:45.82 delay 1 second @21:20:46.84 AFV.select :holdingCoil @21:20:46.95 ATV.dial :air1 @21:20:48.78 AS.reconfigure ASslow1 @21:20:48.83 AS.seek 0.52ml @21:20:53.06 ATV.dial :ASV @21:20:54.76 ASV.dial :SPErecovery @21:20:55.42 AS.seek 0.62ml @21:21:15.81 ATV.dial :waste @21:21:17.74 AS.reconfigure ASnorm @21:21:17.79 AS.seek :empty @21:21:49.42 AFV.select :holdingCoil @21:21:49.44 ATV.dial :waste @21:21:49.46 AS.reconfigure ASconfig @21:21:49.51 AS.seek 0ml @21:21:49.79 AFV.select :carrierSolution @21:21:49.91 delay 2 seconds @21:21:51.94 AS.seek 0.5ml @21:22:20.98 delay 1 second @21:22:21.99 AFV.select :holdingCoil @21:22:22.10 ATV.dial :air3 @21:22:22.80 AS.reconfigure ASslow1 @21:22:22.85 AS.seek 0.52ml @21:22:27.17 ATV.dial :air3 @21:22:27.26 AS.seek 0.53ml @21:22:29.60 delay 2 seconds @21:22:31.61 ATV.dial :bleach @21:22:32.08 AS.seek 0.71ml @21:23:08.56 delay 3 seconds @21:23:11.58 ATV.dial :air3 @21:23:12.15 delay 3 seconds @21:23:15.24 AS.seek 0.735ml @21:23:20.58 delay 3 seconds @21:23:23.60 ATV.dial :bleach @21:23:24.24 AS.seek 0.72ml @21:23:27.56 delay 3 seconds @21:23:30.59 ATV.dial ASV @21:23:31.65 ASV.dial :SPErecovery @21:23:31.74 AS.seek 0.365ml @21:24:43.39 ATV.dial :waste @21:24:45.10 AS.reconfigure ASnorm @21:24:45.15 AS.seek :empty @21:25:03.96 ATV.dial :air3 @21:25:04.64 AS.reconfigure ASslow1 @21:25:04.69 AS.seek 0.009ml @21:25:07.02 delay 2 seconds @21:25:09.03 ATV.dial :bleach @21:25:09.52 AS.seek 0.579ml @21:27:04.48 delay 3 seconds @21:27:07.50 ATV.dial :air3 @21:27:08.04 delay 3 seconds @21:27:11.13 AS.seek 0.604ml @21:27:16.47 delay 3 seconds @21:27:19.49 ATV.dial :bleach @21:27:20.11 AS.seek 0.589ml @21:27:23.35 delay 3 seconds @21:27:26.38 ATV.dial :ASV @21:27:27.41 ASV.dial :wasteR @21:27:27.83 AS.seek 0ml @21:29:26.62 delay 1:00 @21:30:26.64 ATV.dial ASV @21:30:26.66 AFV.select :carrierSolution @21:30:26.76 delay 2 seconds @21:30:28.79 AS.reconfigure ASconfig @21:30:28.84 AS.seek 1ml @21:31:26.60 delay 1 second @21:31:27.61 AFV.select :holdingCoil @21:31:27.72 delay 1 second @21:31:28.74 ASV.dial :wasteR @21:31:29.34 AS.reconfigure ASslow1 @21:31:29.40 AS.seek 0ml @21:34:50.81 delay 10 seconds @21:35:00.85 ATV.dial :air3 @21:35:02.32 AS.seek 0.01ml @21:35:04.62 delay 2 seconds @21:35:06.63 ATV.dial :bleach @21:35:07.27 AS.seek 0.58ml @21:37:02.17 delay 3 seconds @21:37:05.19 ATV.dial :air3 @21:37:05.73 delay 3 seconds @21:37:08.82 AS.seek 0.605ml @21:37:14.12 delay 3 seconds @21:37:17.14 ATV.dial :bleach @21:37:17.75 AS.seek 0.59ml @21:37:21.08 delay 3 seconds @21:37:24.11 ATV.dial ASV @21:37:25.12 ASV.dial :SPEdelivery @21:37:25.59 AS.seek 0ml @21:39:24.51 delay 1:00 @21:40:24.53 AFV.select :carrierSolution @21:40:24.64 delay 2 seconds @21:40:26.67 AS.reconfigure ASconfig @21:40:26.72 AS.seek 1ml @21:41:24.48 delay 1 second @21:41:25.49 AFV.select :holdingCoil @21:41:25.60 delay 1 second @21:41:26.62 ATV.dial ASV @21:41:26.64 ASV.dial :SPEdelivery @21:41:26.66 AS.reconfigure ASslow1 @21:41:26.70 AS.seek 0ml @21:44:48.05 delay 10 seconds @21:44:58.07 ATV.dial :air2 @21:44:58.89 AS.reconfigure ASslow0 @21:44:58.95 AS.seek 0.8ml @21:46:19.80 delay 3 seconds @21:46:22.82 ATV.dial :ASV @21:46:23.77 ASV.dial :SPEdelivery @21:46:23.79 AS.reconfigure ASslow1 @21:46:23.84 AS.seek 0.03ml @21:48:58.98 delay 20 seconds @21:49:19.01 ATV.dial :waste,via: :air3 @21:49:20.77 AS.reconfigure ASconfig @21:49:20.82 AS.seek :empty @21:49:22.91 SPE.coast @21:49:23.00 Removing Bleach from Mixing Coil @21:49:23.02 AFV.select :carrierSolution @21:49:23.12 delay 2 seconds @21:49:25.15 AS.seek 0.5ml @21:49:54.30 delay 1 second @21:49:55.31 AFV.select :holdingCoil @21:49:55.42 ATV.dial :ASV @21:49:57.06 ASV.dial :SPErecovery @21:49:57.70 AS.reconfigure ASslow1 @21:49:57.75 AS.seek 0.73ml @21:50:44.29 ASV.dial :wasteD @21:50:44.86 AS.reconfigure ASnorm @21:50:44.91 AS.seek 0ml @21:51:22.04 AFV.select :carrierSolution @21:51:22.16 delay 2 seconds @21:51:24.19 AS.reconfigure ASconfig @21:51:24.24 AS.seek 0.5ml @21:51:53.28 delay 1 second @21:51:54.29 AFV.select :holdingCoil @21:51:54.40 ATV.dial ASV @21:51:54.41 ASV.dial :SPErecovery @21:51:55.06 AS.reconfigure ASslow0 @21:51:55.11 AS.seek 0.3ml @21:52:15.51 ATV.dial :air2 @21:52:16.52 AS.reconfigure ASslow1 @21:52:16.57 AS.seek 0.32ml @21:52:20.90 ATV.dial ASV @21:52:21.90 AS.reconfigure ASslow0 @21:52:21.95 AS.seek 0.57ml @21:52:47.43 ASV.dial :wasteD @21:52:48.02 AS.reconfigure ASnorm @21:52:48.08 AS.seek 0ml @21:53:17.15 AFV.select :carrierSolution @21:53:17.27 delay 2 seconds @21:53:19.30 AS.reconfigure ASconfig @21:53:19.35 AS.seek 0.5ml @21:53:48.39 delay 1 second @21:53:49.40 AFV.select :holdingCoil @21:53:49.51 ATV.dial ASV @21:53:49.52 ASV.dial :SPErecovery @21:53:50.17 AS.reconfigure ASslow0 @21:53:50.22 AS.seek 0.3ml @21:54:10.68 ATV.dial :air2 @21:54:11.70 AS.reconfigure ASslow1 @21:54:11.75 AS.seek 0.32ml @21:54:16.07 ATV.dial ASV @21:54:17.09 AS.reconfigure ASslow0 @21:54:17.14 AS.seek 0.57ml @21:54:42.62 ASV.dial :wasteD @21:54:43.21 AS.reconfigure ASnorm @21:54:43.27 AS.seek 0ml @21:55:12.34 ATV.dial :air2 @21:55:13.39 AS.reconfigure ASslow0 @21:55:13.45 AS.seek 0.3ml @21:55:44.01 ATV.dial :ASV @21:55:44.96 ASV.dial :wasteD @21:55:44.98 AS.reconfigure ASconfig @21:55:45.03 AS.seek 0.05ml @21:55:59.73 ATV.dial :waste @21:56:01.53 AS.seek 0ml @21:56:04.70 ASV.dial :SPEdelivery @21:56:05.69 deconLysateINT @21:56:05.70 ATV.dial :air3 @21:56:06.44 AS.reconfigure ASslow1 @21:56:06.50 AS.seek 0.01ml @21:56:08.88 delay 2 seconds @21:56:10.90 ATV.dial :bleach @21:56:11.37 AS.seek 0.96ml @21:59:22.68 delay 3 seconds @21:59:25.70 ATV.dial :air3 @21:59:26.25 delay 3 seconds @21:59:29.34 AS.seek 0.985ml @21:59:34.68 delay 3 seconds @21:59:37.70 ATV.dial :bleach @21:59:38.32 AS.seek 0.97ml @21:59:41.63 delay 3 seconds @21:59:44.66 ATV.dial AIV @21:59:45.86 AIV.dial :coilToMFB @21:59:45.87 AWV.select :waste @21:59:45.89 AFV.select :carrierSolution @21:59:46.00 delay 2 seconds @21:59:48.03 AS.reconfigure ASconfig @21:59:48.08 AS.seek 1ml @21:59:50.11 delay 1 second @21:59:51.12 AFV.select :holdingCoil @21:59:51.23 delay 1 second @21:59:52.26 AS.reconfigure ASslow0 @21:59:52.31 AS.seek :empty @22:01:33.28 AFV.select :carrierSolution @22:01:33.39 delay 2 seconds @22:01:35.41 AS.reconfigure ASconfig @22:01:35.46 AS.seek 1ml @22:02:33.25 delay 1 second @22:02:34.27 AFV.select :holdingCoil @22:02:34.38 delay 1 second @22:02:35.41 AS.reconfigure ASslow0 @22:02:35.46 AS.seek :empty @22:04:16.42 AFV.select :carrierSolution @22:04:16.54 delay 2 seconds @22:04:18.57 AS.reconfigure ASconfig @22:04:18.62 AS.seek 1ml @22:05:16.43 delay 1 second @22:05:17.44 AFV.select :holdingCoil @22:05:17.55 delay 1 second @22:05:18.58 AS.reconfigure ASslow0 @22:05:18.62 AS.seek :empty @22:06:59.59 ATV.dial :air2 @22:07:00.25 AS.reconfigure ASslow1 @22:07:00.31 AS.seek 0.009ml @22:07:02.65 AS.reconfigure ASslow0 @22:07:02.70 AS.seek 1ml @22:08:42.64 ATV.dial AIV @22:08:43.41 AS.seek 0.02ml @22:10:22.28 ATV.dial :waste @22:10:23.96 AS.seek 0ml @22:10:26.26 AWV.select :air @22:10:26.43 Cleaning PCR @22:10:26.44 AFV.select :carrierSolution @22:10:26.55 delay 2 seconds @22:10:28.58 AS.reconfigure ASconfig @22:10:28.63 AS.seek 0.5ml @22:10:57.68 delay 1 second @22:10:58.69 AFV.select :holdingCoil @22:10:58.80 ASV.dial :waste1 @22:10:59.16 ATV.dial :air3 @22:10:59.82 AS.reconfigure ASslow1 @22:10:59.88 AS.seek 0.51ml @22:11:02.13 delay 2 seconds @22:11:04.14 ATV.dial :bleach @22:11:04.59 AS.seek 0.81ml @22:12:05.24 delay 3 seconds @22:12:08.26 ATV.dial :air3 @22:12:08.79 delay 3 seconds @22:12:11.87 AS.seek 0.835ml @22:12:17.18 delay 3 seconds @22:12:20.80 ATV.dial :bleach @22:12:21.42 AS.seek 0.82ml @22:12:24.71 delay 3 seconds @22:12:27.73 ARV.dial :waste @22:12:28.34 delay 3 seconds @22:12:31.36 ATV.dial :ARV @22:12:32.23 AS.reconfigure ASconfig @22:12:32.28 AS.seek 0.47ml @22:12:52.68 AFV.select :carrierSolution @22:12:52.80 delay 2 seconds @22:12:54.83 AS.seek 1ml @22:13:25.60 delay 1 second @22:13:26.61 AFV.select :holdingCoil @22:13:26.72 delay 1 second @22:13:27.75 AS.reconfigure ASfast @22:13:27.80 AS.seek 0ml @22:13:55.14 AFV.select :carrierSolution @22:13:55.26 delay 2 seconds @22:13:57.29 AS.reconfigure ASconfig @22:13:57.34 AS.seek 1ml @22:14:55.13 delay 1 second @22:14:56.14 AFV.select :holdingCoil @22:14:56.25 delay 1 second @22:14:57.28 AS.reconfigure ASfast @22:14:57.33 AS.seek 0ml @22:15:24.65 AFV.select :carrierSolution @22:15:24.77 delay 2 seconds @22:15:26.80 AS.reconfigure ASconfig @22:15:26.85 AS.seek 1ml @22:16:24.65 delay 1 second @22:16:25.66 AFV.select :holdingCoil @22:16:25.77 delay 1 second @22:16:26.80 AS.reconfigure ASfast @22:16:26.85 AS.seek 0ml @22:16:54.19 ARV.dial between carrier and air1 @22:16:54.70 ATV.dial :air3 @22:16:55.94 AS.reconfigure ASslow1 @22:16:55.99 AS.seek 0.01ml @22:16:58.35 delay 2 seconds @22:17:00.36 ATV.dial :bleach @22:17:00.98 AS.seek 0.81ml @22:19:42.12 delay 3 seconds @22:19:45.14 ATV.dial :air3 @22:19:45.68 delay 3 seconds @22:19:48.77 AS.seek 0.835ml @22:19:54.09 delay 3 seconds @22:19:57.11 ATV.dial :bleach @22:19:57.72 AS.seek 0.82ml @22:20:00.96 delay 3 seconds @22:20:03.98 ATV.dial PCR @22:20:04.38 AS.reconfigure ASconfig @22:20:04.43 AS.seek :empty @22:20:51.89 PCR.program :bleachProfile @22:20:51.95 PCR program should complete in < 2:45 @22:20:51.96 PCR.run 10:00 @22:24:11.96 PCR.off @22:24:11.99 AFV.select :carrierSolution @22:24:12.10 delay 2 seconds @22:24:14.13 AS.seek 1ml @22:25:11.91 delay 1 second @22:25:12.93 AFV.select :holdingCoil @22:25:13.04 delay 1 second @22:25:14.07 AS.reconfigure ASfast @22:25:14.12 AS.seek 0ml @22:25:41.46 delay 3 seconds @22:25:44.47 AFV.select :carrierSolution @22:25:44.58 delay 2 seconds @22:25:46.61 AS.reconfigure ASconfig @22:25:46.66 AS.seek 1ml @22:26:44.45 delay 1 second @22:26:45.46 AFV.select :holdingCoil @22:26:45.57 delay 1 second @22:26:46.60 AS.reconfigure ASfast @22:26:46.65 AS.seek 0ml @22:27:14.01 delay 3 seconds @22:27:17.03 AFV.select :carrierSolution @22:27:17.14 delay 2 seconds @22:27:19.17 AS.reconfigure ASconfig @22:27:19.23 AS.seek 1ml @22:28:17.03 delay 1 second @22:28:18.04 AFV.select :holdingCoil @22:28:18.15 delay 1 second @22:28:19.18 AS.reconfigure ASfast @22:28:19.23 AS.seek 0ml @22:28:46.57 delay 3 seconds @22:28:49.59 AFV.select :carrierSolution @22:28:49.70 delay 2 seconds @22:28:51.73 AS.reconfigure ASconfig @22:28:51.78 AS.seek 1ml @22:29:49.53 delay 1 second @22:29:50.54 AFV.select :holdingCoil @22:29:50.65 delay 1 second @22:29:51.68 AS.reconfigure ASfast @22:29:51.73 AS.seek 0ml @22:30:19.67 delay 3 seconds @22:30:22.69 AFV.select :carrierSolution @22:30:22.80 delay 2 seconds @22:30:24.83 AS.reconfigure ASconfig @22:30:24.89 AS.seek 1ml @22:31:22.68 delay 1 second @22:31:23.69 AFV.select :holdingCoil @22:31:23.80 delay 1 second @22:31:24.83 AS.reconfigure ASnorm @22:31:24.88 AS.seek 0ml @22:32:15.59 AFV.select :carrierSolution @22:32:15.70 delay 2 seconds @22:32:17.73 AS.reconfigure ASconfig @22:32:17.78 AS.seek 1ml @22:33:15.58 delay 1 second @22:33:16.59 AFV.select :holdingCoil @22:33:16.70 delay 1 second @22:33:17.72 ATV.dial :waste @22:33:18.89 AS.reconfigure ASnorm @22:33:18.94 AS.seek 0.5ml @22:33:44.49 ATV.dial :waste2 @22:33:45.73 AS.seek 0ml @22:34:11.25 AS.coast @22:34:11.29 SS.coast @22:34:11.33 CC.coast @22:34:11.37 CS.coast @22:34:11.41 PC.coast @22:34:11.45 PS.coast @22:34:11.49 Forearm.coast @22:34:11.54 Elbow.coast @22:34:11.93 Gate.power :core,:OFF @22:34:11.94 Gate.power :raw,:OFF @22:34:11.95 Shutdown @22:34:11.97 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @06:33:29.68CEST22-Oct-18 Session Begun @06:33:42.60 -> ESP.wakeup @06:33:42.66 Powering core bus @06:33:47.49 Gate.power :raw,:ON @06:33:48.17 SPE.coast @06:33:48.26 SPE.configure SPEconfig @06:33:48.38 AS.configure ASconfig @06:33:48.51 SS.configure SSconfig @06:33:48.64 CC.configure CCconfig @06:33:48.76 CS.configure CSconfig @06:33:48.88 PC.configure PCconfig @06:33:49.00 PS.configure PSconfig @06:33:49.15 Forearm.configure LooseForearm @06:33:49.27 Elbow.configure LooseElbow @06:33:49.41 SC.configure SCloose @06:33:49.53 SE.configure SEconfig @06:33:49.60 CH.coast @06:33:49.69 CH.configure CHconfig @06:33:49.75 PH.coast @06:33:49.84 PH.configure PHconfig @06:33:49.91 PCR.off @06:33:49.96 PCR.pidtemp= 50,0.1,0,300,25,25 @06:33:50.00 PCR.caltemp= 2.7073,0.0373,0 @06:33:50.05 All dwarf controllers running firmware version 3.75 @06:33:50.19 Valve.reset @06:33:50.38 PCR.off @06:33:50.44 PCR.pidtemp= 50,0.1,0,300,25,25 @06:33:50.48 PCR.caltemp= 2.7073,0.0373,0 @06:33:50.72 PSV.dial :air @06:33:50.75 Intake.select :close @06:33:50.78 Exhaust.select :close @06:33:50.81 CTV.dial :air @06:33:50.96 CSV.dial :waste1 @06:33:51.10 Forearm.reconfigure LooseForearm @06:33:51.17 Forearm.home.seek :retract @06:33:51.26 SSV.dial :spit,avoiding: :exhaust @06:33:51.37 PS.reconfigure PSconfig @06:33:52.05 Hand.close @06:33:52.09 PS.home.seek 0ml @06:33:52.13 Elbow.jog 1500 @06:33:52.33 CS.reconfigure CSfast @06:33:52.36 SS.reconfigure SSconfig @06:33:52.49 CS.home.seek 0ml @06:33:52.51 SS.home.seek 0ml @06:33:52.97 Elbow.reconfigure LooseElbow @06:33:53.04 Elbow.home.seek :clear @06:33:56.28 SE.reconfigure SEconfig @06:33:56.35 SE.home.seek :down @06:33:57.08 SC.reconfigure SCsloppy @06:33:57.16 SC.home.seek :initial @06:34:01.36 PS.coast @06:34:02.77 Forearm.seek :retract @06:34:02.88 Elbow.seek :hammer @06:34:04.02 Forearm.reconfigure TightForearm @06:34:04.10 Forearm.seek 5 + 350 counts @06:34:05.21 Forearm.seek :retract @06:34:06.42 PC.reconfigure PCconfig @06:34:06.44 CC.reconfigure CCconfig @06:34:06.57 PC.home.seek :open @06:34:06.60 CC.home.seek :open @06:34:29.62 -> MFB.ready! @06:34:29.73 Readying the MFB @06:34:29.75 Homing MFB Syringe @06:34:29.77 AFV.select :holdingCoil @06:34:29.95 ATV.dial :air2 @06:34:30.52 AS.reconfigure ASconfig @06:34:30.59 AS.home.seek :home @06:34:36.12 AFV.select :carrierSolution @06:34:36.26 delay 2 seconds @06:34:38.29 AS.seek 1ml @06:35:31.21 delay 1 second @06:35:32.23 AFV.select :holdingCoil @06:35:32.36 delay 1 second @06:35:33.39 ATV.dial :waste @06:35:34.41 AS.reconfigure ASnorm @06:35:34.49 AS.home.seek :empty @06:36:27.07 ATV.dial :waste @06:36:27.09 ARV.dial between carrier and air1 @06:36:27.12 Errno::EPIPE in mwj's_reader -- Broken pipe @06:36:27.15 Session Ended @06:38:21.84 Session Begun @06:38:28.15 -> showlog 10 @06:38:55.25 -> MFB.ready? @06:38:59.99 -> AS @06:39:15.04 -> ATV.ARV @06:39:15.06 ATV.dial ARV @06:39:40.44 -> ARV.air3 @06:39:40.46 ARV.dial :air3 @06:40:17.92 -> AS.pull 0.2, ASslow1 @06:40:18.03 AS.reconfigure ASslow1 @06:40:18.10 AS.seek 0.199ml @06:41:54.47 -> ARV.PP4 @06:41:54.49 ARV.dial :PP4 @06:42:37.78 -> AS.pull 0.05, ASslow1 @06:42:37.89 AS.seek 0.249ml @06:44:54.16 Session Ended @06:46:11.31 Session Begun @06:46:17.67 -> showlog 10 @06:46:27.93 -> AS @06:46:44.13 -> ARV @06:46:51.67 -> ARV.air3 @06:46:51.69 ARV.dial :air3 @06:46:54.84 -> ATV @06:47:10.88 -> AS.pull 0.05, ASslow1 @06:47:10.98 AS.seek 0.299ml @06:47:31.37 -> ARV.MM2 @06:47:31.39 ARV.dial :MM2 @06:47:54.58 -> AS.pull 0.02, ASslow1 @06:47:54.69 AS.seek 0.319ml @06:48:13.15 -> ARV.air2 @06:48:13.18 ARV.dial :air2 @06:48:40.03 -> AS.pull 0.05, ASslow1 @06:48:40.13 AS.seek 0.369ml @06:48:59.73 -> ARV.PP2 @06:48:59.75 ARV.dial :PP2 @06:49:17.09 -> AS.pull 0.02, ASslow1 @06:49:17.19 AS.seek 0.389ml @06:49:28.87 -> ARV.air2 @06:49:28.90 ARV.dial :air2 @06:49:44.93 -> AS.pull 0.2, ASslow1 @06:49:45.58 AS.seek 0.589ml @06:50:32.17 -> ATV.waste @06:50:32.18 ATV.dial :waste @06:50:38.44 -> AS.empty @06:50:38.46 AS.reconfigure ASconfig @06:50:38.55 AS.seek :empty @06:52:31.92 -> pcrDecon @06:52:32.02 Cleaning PCR @06:52:32.03 AFV.select :carrierSolution @06:52:32.18 delay 2 seconds @06:52:34.21 AS.seek 0.5ml @06:53:03.34 delay 1 second @06:53:04.35 AFV.select :holdingCoil @06:53:04.48 ASV.dial :waste1 @06:53:04.59 ATV.dial :air3 @06:53:05.44 AS.reconfigure ASslow1 @06:53:05.51 AS.seek 0.51ml @06:53:07.85 delay 2 seconds @06:53:09.87 ATV.dial :bleach @06:53:10.35 AS.seek 0.81ml @06:53:12.01 Session Begun @06:54:10.96 delay 3 seconds @06:54:13.98 ATV.dial :air3 @06:54:14.56 delay 3 seconds @06:54:17.65 AS.seek 0.835ml @06:54:23.07 delay 3 seconds @06:54:26.09 ATV.dial :bleach @06:54:26.75 AS.seek 0.82ml @06:54:30.09 delay 3 seconds @06:54:33.12 ARV.dial :waste @06:54:34.59 delay 3 seconds @06:54:37.61 ATV.dial :ARV @06:54:38.52 AS.reconfigure ASconfig @06:54:38.60 AS.seek 0.47ml @06:54:58.99 AFV.select :carrierSolution @06:54:59.15 delay 2 seconds @06:55:01.18 AS.seek 1ml @06:55:31.97 delay 1 second @06:55:32.99 AFV.select :holdingCoil @06:55:33.14 delay 1 second @06:55:34.17 AS.reconfigure ASfast @06:55:34.25 AS.seek 0ml @06:56:01.63 AFV.select :carrierSolution @06:56:01.80 delay 2 seconds @06:56:03.83 AS.reconfigure ASconfig @06:56:03.91 AS.seek 1ml @06:57:01.75 delay 1 second @06:57:02.77 AFV.select :holdingCoil @06:57:02.91 delay 1 second @06:57:03.94 AS.reconfigure ASfast @06:57:04.02 AS.seek 0ml @06:57:31.41 AFV.select :carrierSolution @06:57:31.56 delay 2 seconds @06:57:33.59 AS.reconfigure ASconfig @06:57:33.67 AS.seek 1ml @06:58:31.50 delay 1 second @06:58:32.51 AFV.select :holdingCoil @06:58:32.65 delay 1 second @06:58:33.68 AS.reconfigure ASfast @06:58:33.77 AS.seek 0ml @06:59:01.14 ARV.dial between carrier and air1 @06:59:01.69 ATV.dial :air3 @06:59:02.96 AS.reconfigure ASslow1 @06:59:03.04 AS.seek 0.01ml @06:59:05.45 delay 2 seconds @06:59:07.46 ATV.dial :bleach @06:59:08.10 AS.seek 0.81ml @07:01:49.34 delay 3 seconds @07:01:52.36 ATV.dial :air3 @07:01:52.94 delay 3 seconds @07:01:56.03 AS.seek 0.835ml @07:02:01.37 delay 3 seconds @07:02:04.39 ATV.dial :bleach @07:02:05.04 AS.seek 0.82ml @07:02:08.36 delay 3 seconds @07:02:11.38 ATV.dial PCR @07:02:11.81 AS.reconfigure ASconfig @07:02:11.89 AS.seek :empty @07:02:59.36 PCR.program :bleachProfile @07:02:59.46 PCR program should complete in < 2:45 @07:02:59.47 PCR.run 10:00 @07:06:31.68 PCR.off @07:06:31.72 AFV.select :carrierSolution @07:06:31.89 delay 2 seconds @07:06:32.04 Session Ended @07:06:32.10 Errno::EPIPE in mwj's_reader -- Broken pipe @07:06:32.12 Session Ended @07:07:53.16 Session Begun @07:08:01.43 -> showlog 10 @07:09:30.55 -> showlog 10 @07:10:21.64 -> AS.pull 1, ASfast @07:10:21.75 AS.reconfigure ASfast @07:10:21.83 AS.seek 0.999ml @07:11:51.00 -> AFV.holdingCoil @07:11:51.02 AFV.select :holdingCoil @07:12:01.62 -> AS.empty @07:12:01.64 AS.reconfigure ASconfig @07:12:01.73 AS.seek :empty @07:13:21.31 -> AFV.carrierSolution @07:13:21.33 AFV.select :carrierSolution @07:13:39.90 -> AS.pull 1, ASfast @07:13:40.01 AS.reconfigure ASfast @07:13:40.08 AS.seek 0.999ml @07:14:22.18 -> AFV.holdingCoil @07:14:22.19 AFV.select :holdingCoil @07:14:30.74 -> AS.empty @07:14:30.77 AS.reconfigure ASconfig @07:14:30.86 AS.seek :empty @07:15:41.19 -> AFV.carrierSolution @07:15:41.20 AFV.select :carrierSolution @07:16:01.62 -> AS.pull 1, ASfast @07:16:01.73 AS.reconfigure ASfast @07:16:01.81 AS.seek 0.999ml @07:16:42.35 -> AFV.holdingCoil @07:16:42.36 AFV.select :holdingCoil @07:17:12.19 -> ASV @07:17:16.49 -> ATV @07:17:20.25 -> AFV @07:17:25.95 -> AS @07:18:57.25 Session Begun @07:19:03.92 -> showlog 10 @07:19:11.07 -> AS @07:19:16.39 -> ATV @07:19:20.32 -> AFV @07:19:25.89 -> ASV @07:19:28.27 Session Ended @07:19:37.68 -> AS.empty @07:19:37.71 AS.reconfigure ASconfig @07:19:37.80 AS.seek :empty @07:20:53.22 -> AFV.carrierSolution @07:20:53.24 AFV.select :carrierSolution @07:21:04.61 -> AS.pull 1, ASfast @07:21:04.71 AS.reconfigure ASfast @07:21:04.79 AS.seek 0.999ml @07:21:41.52 -> AFV.holdingCoil @07:21:41.53 AFV.select :holdingCoil @07:21:49.69 -> AS.empty @07:21:49.71 AS.reconfigure ASconfig @07:21:49.79 AS.seek :empty @07:24:04.37 Session Ended @07:24:44.07 Session Begun @07:24:50.83 -> showlog 10 @07:24:57.77 -> AS @07:25:04.54 -> AFV @07:25:14.61 -> AFV.carrierSolution @07:25:14.63 AFV.select :carrierSolution @07:25:28.84 -> AS.pull 1, ASfast @07:25:28.95 AS.reconfigure ASfast @07:25:29.03 AS.seek 0.999ml @07:26:07.47 -> AFV.holdingCoil @07:26:07.49 AFV.select :holdingCoil @07:26:21.61 -> AS.empty @07:26:21.64 AS.reconfigure ASconfig @07:26:21.72 AS.seek :empty @07:27:32.59 -> ATV.waste @07:27:32.61 ATV.dial :waste @07:27:49.00 -> ESP.shutdown @07:27:49.04 AS.coast @07:27:49.08 Asleep / Shutdown @07:27:49.15 SS.coast @07:27:49.21 CC.coast @07:27:49.26 CS.coast @07:27:49.32 PC.coast @07:27:49.38 Forearm.coast @07:27:49.44 Elbow.coast @07:27:49.50 SC.coast @07:27:49.56 SE.coast @07:27:49.97 Gate.power :core,:OFF @07:27:49.99 Gate.power :raw,:OFF @07:27:50.02 Shutdown @07:28:02.61 -> exit @07:28:02.63 Session Ended @14:00:53.15CEST23-Oct-18
Powering core bus @14:00:58.45 Gate.power :raw,:ON @14:00:59.56 SPE.coast @14:00:59.62 SPE.configure SPEconfig @14:00:59.70 AS.configure ASconfig @14:00:59.79 SS.configure SSconfig @14:00:59.88 CC.configure CCconfig @14:00:59.97 CS.configure CSconfig @14:01:00.05 PC.configure PCconfig @14:01:00.15 PS.configure PSconfig @14:01:00.25 Forearm.configure LooseForearm @14:01:00.33 Elbow.configure LooseElbow @14:01:00.44 SC.configure SCloose @14:01:00.53 SE.configure SEconfig @14:01:00.57 CH.coast @14:01:00.61 CH.configure CHconfig @14:01:00.65 PH.coast @14:01:00.69 PH.configure PHconfig @14:01:00.73 PCR.off @14:01:00.76 PCR.pidtemp= 50,0.1,0,300,25,25 @14:01:00.79 PCR.caltemp= 2.7073,0.0373,0 @14:01:00.83 All dwarf controllers running firmware version 3.75 @14:01:00.94 Valve.reset @14:01:01.09 PCR.off @14:01:01.11 PCR.pidtemp= 50,0.1,0,300,25,25 @14:01:01.14 PCR.caltemp= 2.7073,0.0373,0 @14:01:01.24 Intake.select :close @14:01:01.28 Exhaust.select :close @14:01:01.36 CTV.dial :air @14:01:01.44 PSV.dial :air @14:01:01.55 CSV.dial :waste1 @14:01:01.58
Forearm.reconfigure LooseForearm @14:01:01.68 Forearm.home.seek :retract @14:01:01.72 SSV.dial :spit,avoiding: :exhaust @14:01:01.76 PS.reconfigure PSconfig @14:01:01.86 PS.home.seek 0ml @14:01:01.96 CS.reconfigure CSfast @14:01:01.99 SS.reconfigure SSconfig @14:01:02.08 SS.home.seek 0ml @14:01:02.10 CS.home.seek 0ml @14:01:02.42 Hand.close @14:01:02.47
Elbow.jog 1500 @14:01:03.00 Elbow.reconfigure LooseElbow @14:01:03.04 Elbow.home.seek :clear @14:01:07.71 SE.reconfigure SEconfig @14:01:07.75 SE.home.seek :down @14:01:08.43 SC.reconfigure SCsloppy @14:01:08.48 SC.home.seek :initial @14:01:10.94 PS.coast @14:01:12.49
Forearm.seek :retract @14:01:12.65 Elbow.seek :hammer @14:01:13.85 Forearm.reconfigure TightForearm @14:01:13.89 Forearm.seek 5 + 350 counts @14:01:14.90 Forearm.seek :retract @14:01:16.05 PC.reconfigure PCconfig @14:01:16.07
CC.reconfigure CCconfig @14:01:16.15 PC.home.seek :open @14:01:16.18
CC.home.seek :open @14:01:19.64 GoodNews.email "At 14:01:19 began in tube 4: mbaPhase\n/home/esp/esp2/mission/18tundeployv4.rb:127\n{1 => 22, 2 => 22, 3 => 7, 4 => 15}\nCan@14:01:19, 13.0C, 73% humidity, 14.3psia, 12.822V, 0.714A, 0.714A avg, 9.15W",Subject: "Started 18tundeployv4 phase mbaPhase" @14:01:19.66
Phase [mbaPhase] @14:01:19.72 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:01:19.74CEST23-Oct-18 ==================> STARTED Superphase (phase #4) <=== @14:01:19.76 Retry #1 of 10 after 10:00 @14:01:19.77
Starting initialPurge @14:01:19.80 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:01:19.86 Hand.open @14:01:20.77 Elbow.reconfigure TightElbow @14:01:20.81 Elbow.seek FlushPuck::Garage @14:01:22.60 Elbow.reconfigure LooseElbow @14:01:22.69 Forearm.reconfigure LooseForearm @14:01:22.73 Forearm.seek :garage @14:01:23.31 Slide::Error in MAIN -- Forearm speedErr at home - 2702 counts @14:01:23.32 Retry #1 of 3 Hand.open @14:01:23.60 Elbow.reconfigure TightElbow @14:01:23.64 Elbow.seek FlushPuck::Garage @14:01:23.85 Elbow.reconfigure LooseElbow @14:01:23.95 Forearm.seek :garage @14:01:24.27 Slide::Error in MAIN -- Forearm speedErr at home - 2727 counts @14:01:24.28 Retry #2 of 3 @14:01:24.29 Hand.open @14:01:24.60 Elbow.reconfigure TightElbow @14:01:24.64 Elbow.seek FlushPuck::Garage @14:01:24.76 Elbow.reconfigure LooseElbow @14:01:24.85 Forearm.seek :garage @14:01:25.17 Slide::Error in MAIN -- Forearm speedErr at home - 2736 counts @14:01:25.18 Retry #3 of 3 @14:01:25.19 Hand.open @14:01:25.47 Elbow.reconfigure TightElbow @14:01:25.51 Elbow.seek FlushPuck::Garage @14:01:25.63 Elbow.reconfigure LooseElbow @14:01:25.72 Forearm.seek :garage @14:01:26.06 Slide::Error in MAIN -- Forearm speedErr at home - 2745 counts Waiting to Resume from Checkpoint "Forearm speedErr at home - 2745 counts" @14:11:19.78 GoodNews.email "At 14:01:19 began in tube 4: mbaPhase\n/home/esp/esp2/mission/18tundeployv4.rb:127\n{1 => 22, 2 => 22, 3 => 7, 4 => 15}\nCan@14:01:19, 13.0C, 73% humidity, 14.3psia, 12.822V, 0.714A, 0.714A avg, 9.15W",Subject: "Started 18tundeployv4 phase mbaPhase" @14:11:19.81 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:11:19.83 Retry #2 of 10 after 10:00 @14:21:19.86 GoodNews.email "At 14:01:19 began in tube 4: mbaPhase\n/home/esp/esp2/mission/18tundeployv4.rb:127\n{1 => 22, 2 => 22, 3 => 7, 4 => 15}\nCan@14:01:19, 13.0C, 73% humidity, 14.3psia, 12.822V, 0.714A, 0.714A avg, 9.15W",Subject: "Started 18tundeployv4 phase mbaPhase" @14:21:19.88 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:21:19.90 Retry #3 of 10 after 10:00 @14:31:19.92 GoodNews.email "At 14:01:19 began in tube 4: mbaPhase\n/home/esp/esp2/mission/18tundeployv4.rb:127\n{1 => 22, 2 => 22, 3 => 7, 4 => 15}\nCan@14:01:19, 13.0C, 73% humidity, 14.3psia, 12.822V, 0.714A, 0.714A avg, 9.15W",Subject: "Started 18tundeployv4 phase mbaPhase" @14:31:19.95 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:31:19.97 Retry #4 of 10 after 10:00 @14:41:19.99 GoodNews.email "At 14:01:19 began in tube 4: mbaPhase\n/home/esp/esp2/mission/18tundeployv4.rb:127\n{1 => 22, 2 => 22, 3 => 7, 4 => 15}\nCan@14:01:19, 13.0C, 73% humidity, 14.3psia, 12.822V, 0.714A, 0.714A avg, 9.15W",Subject: "Started 18tundeployv4 phase mbaPhase" @14:41:20.02 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:41:20.04 Retry #5 of 10 after 10:00 @14:51:20.05 GoodNews.email "At 14:01:19 began in tube 4: mbaPhase\n/home/esp/esp2/mission/18tundeployv4.rb:127\n{1 => 22, 2 => 22, 3 => 7, 4 => 15}\nCan@14:01:19, 13.0C, 73% humidity, 14.3psia, 12.822V, 0.714A, 0.714A avg, 9.15W",Subject: "Started 18tundeployv4 phase mbaPhase" @14:51:20.08 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @14:51:20.10 Retry #6 of 10 after 10:00 @15:01:20.11 GoodNews.email "At 14:01:19 began in tube 4: mbaPhase\n/home/esp/esp2/mission/18tundeployv4.rb:127\n{1 => 22, 2 => 22, 3 => 7, 4 => 15}\nCan@14:01:19, 13.0C, 73% humidity, 14.3psia, 12.822V, 0.714A, 0.714A avg, 9.15W",Subject: "Started 18tundeployv4 phase mbaPhase" @15:01:20.14 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:01:20.16 Retry #7 of 10 after 10:00 @15:11:20.18 GoodNews.email "At 14:01:19 began in tube 4: mbaPhase\n/home/esp/esp2/mission/18tundeployv4.rb:127\n{1 => 22, 2 => 22, 3 => 7, 4 => 15}\nCan@14:01:19, 13.0C, 73% humidity, 14.3psia, 12.822V, 0.714A, 0.714A avg, 9.15W",Subject: "Started 18tundeployv4 phase mbaPhase" @15:11:20.21 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:11:20.23 Retry #8 of 10 after 10:00 @15:21:20.24 GoodNews.email "At 14:01:19 began in tube 4: mbaPhase\n/home/esp/esp2/mission/18tundeployv4.rb:127\n{1 => 22, 2 => 22, 3 => 7, 4 => 15}\nCan@14:01:19, 13.0C, 73% humidity, 14.3psia, 12.822V, 0.714A, 0.714A avg, 9.15W",Subject: "Started 18tundeployv4 phase mbaPhase" @15:21:20.27 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:21:20.29 Retry #9 of 10 after 10:00 @15:31:20.30 GoodNews.email "At 14:01:19 began in tube 4: mbaPhase\n/home/esp/esp2/mission/18tundeployv4.rb:127\n{1 => 22, 2 => 22, 3 => 7, 4 => 15}\nCan@14:01:19, 13.0C, 73% humidity, 14.3psia, 12.822V, 0.714A, 0.714A avg, 9.15W",Subject: "Started 18tundeployv4 phase mbaPhase" @15:31:20.33 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:31:20.35 Retry #10 of 10 after 10:00 @15:41:20.36 GoodNews.email "At 14:01:19 began in tube 4: mbaPhase\n/home/esp/esp2/mission/18tundeployv4.rb:127\n{1 => 22, 2 => 22, 3 => 7, 4 => 15}\nCan@14:01:19, 13.0C, 73% humidity, 14.3psia, 12.822V, 0.714A, 0.714A avg, 9.15W",Subject: "Started 18tundeployv4 phase mbaPhase" @15:41:20.40 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:41:20.41 BadNews.email "Forearm speedErr at home - 2745 counts",Subject: "Slide::Error in MAIN" @15:41:20.43 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:41:20.44 Retry #1 of 10 after 10:00 @15:51:20.46 BadNews.email "Forearm speedErr at home - 2745 counts",Subject: "Slide::Error in MAIN" @15:51:20.49 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @15:51:20.50 Retry #2 of 10 after 10:00 @16:01:20.52 BadNews.email "Forearm speedErr at home - 2745 counts",Subject: "Slide::Error in MAIN" @16:01:20.55 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @16:01:20.56 Retry #3 of 10 after 10:00 @16:11:20.58 BadNews.email "Forearm speedErr at home - 2745 counts",Subject: "Slide::Error in MAIN" @16:11:20.61 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @16:11:20.63 Retry #4 of 10 after 10:00 @16:21:20.64 BadNews.email "Forearm speedErr at home - 2745 counts",Subject: "Slide::Error in MAIN" @16:21:20.67 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @16:21:20.68 Retry #5 of 10 after 10:00 @16:31:20.70 BadNews.email "Forearm speedErr at home - 2745 counts",Subject: "Slide::Error in MAIN" @16:31:20.73 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @16:31:20.75 Retry #6 of 10 after 10:00 @16:41:20.76 BadNews.email "Forearm speedErr at home - 2745 counts",Subject: "Slide::Error in MAIN" @16:41:20.79 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @16:41:20.81 Retry #7 of 10 after 10:00 @16:51:20.82 BadNews.email "Forearm speedErr at home - 2745 counts",Subject: "Slide::Error in MAIN" @16:51:20.85 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @16:51:20.87 Retry #8 of 10 after 10:00 @17:01:20.88 BadNews.email "Forearm speedErr at home - 2745 counts",Subject: "Slide::Error in MAIN" @17:01:20.91 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @17:01:20.93 Retry #9 of 10 after 10:00 @17:11:20.94 BadNews.email "Forearm speedErr at home - 2745 counts",Subject: "Slide::Error in MAIN" @17:11:20.97 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @17:11:20.98 Retry #10 of 10 after 10:00 @17:21:21.00 BadNews.email "Forearm speedErr at home - 2745 counts",Subject: "Slide::Error in MAIN" @17:21:21.03 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:24:20.99CET09-Nov-18 Session Begun @19:24:27.41 -> showlog 500 @19:32:07.68 -> can @19:44:22.85 -> quit @19:44:22.87 Session Ended @08:37:50.73CET12-Nov-18 Session Begun @08:37:54.11 -> can @08:47:06.05 -> showlog 200 @09:16:19.81 -> exit @09:16:19.83 Session Ended @17:07:01.16CET19-Nov-18 LoadError in MAIN -- no such file to load -- axiskernel @17:07:01.19 SystemExit in MAIN -- exit @17:25:00.98CET19-Nov-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @17:25:02.65 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @17:25:02.97 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @17:25:08.05 Polling Can every 10:00 @17:25:09.80 SPE.coast @17:25:09.85 SPE.configure SPEconfig @17:25:09.93 AS.configure ASconfig @17:25:10.02 SS.reconfigure SSconfig @17:25:10.05 SS.hold @17:25:10.11 SS.configure SSconfig @17:25:10.19 CC.reconfigure CCconfig @17:25:10.23 CC.hold @17:25:10.28 CC.configure CCconfig @17:25:10.35 CS.reconfigure CSconfig @17:25:10.40 CS.hold @17:25:10.47 CS.configure CSconfig @17:25:10.55 PC.reconfigure PCconfig @17:25:10.59 PC.hold @17:25:10.99 PC.configure PCconfig @17:25:11.07 PS.configure PSconfig @17:25:11.17 Forearm.reconfigure LooseForearm @17:25:11.21 Forearm.hold @17:25:11.30 Forearm.configure LooseForearm @17:25:11.48 Elbow.reconfigure LooseElbow @17:25:11.54 Elbow.hold @17:25:11.64 Elbow.configure LooseElbow @17:25:11.84 SC.reconfigure SCloose @17:25:11.92 SC.hold @17:25:12.01 SC.configure SCloose @17:25:12.11 SE.reconfigure SEconfig @17:25:12.15 SE.hold @17:25:12.21 SE.configure SEconfig @17:25:12.25 CH.coast @17:25:12.29 CH.configure CHconfig @17:25:12.32 PH.coast @17:25:12.37 PH.configure PHconfig @17:25:12.41 PCR.off @17:25:12.43 PCR.pidtemp= 50,0.1,0,300,25,25 @17:25:12.46 PCR.caltemp= 2.7073,0.0373,0 @17:25:12.49 All dwarf controllers running firmware version 3.75 @17:25:12.63 Loading /home/esp/esp2/mission/18tun_postDeploy.rb mission @17:25:12.69 GoodNews.email "Starting 18tun_postDeploy w/pucks in tube 4\n{}\nCan@17:25:08, 20.7C, 39% humidity, 14.8psia, 12.001V, 0.626A, 0.626A avg, 7.51W",Subject: "18tun_postDeploy begun" @17:25:12.73
Preparing to sleep until +0:00:15 @17:25:12.77 SS.coast @17:25:12.79
Asleep til +0:00:15 @17:25:12.83 Continuing at 17:25 @17:25:12.85 CC.coast @17:25:12.93 CS.coast @17:25:12.96 PC.coast @17:25:13.00 Forearm.coast @17:25:13.03 Elbow.coast @17:25:13.08 SC.coast @17:25:13.11 SE.coast @17:25:13.49 Gate.power :core,:OFF @17:25:13.51 Gate.power :raw,:OFF Shutdown @17:25:14.73 Session Begun @17:25:21.40 GoodNews.email "Then, in tube 4: postDeployPhase\n/home/esp/esp2/mission/18tun_postDeploy.rb:104\n{}\nCan@17:25:12, 20.7C, 39% humidity, 14.8psia, 12.001V, 0.626A, 0.626A avg, 7.51W",Subject: "Asleep until 17:25" @17:25:25.68 -> showlog 10 @17:25:27.66
Powering core bus @17:25:32.57 Gate.power :raw,:ON @17:25:33.26 SPE.coast @17:25:33.33 SPE.configure SPEconfig @17:25:33.44 AS.configure ASconfig @17:25:33.54 SS.configure SSconfig @17:25:33.65 CC.configure CCconfig @17:25:33.75 CS.configure CSconfig @17:25:33.85 PC.configure PCconfig @17:25:33.97 PS.configure PSconfig @17:25:34.09 Forearm.configure LooseForearm @17:25:34.21 Elbow.configure LooseElbow @17:25:34.34 SC.configure SCloose @17:25:34.45 SE.configure SEconfig @17:25:34.51 CH.coast @17:25:34.57 CH.configure CHconfig @17:25:34.62 PH.coast @17:25:34.69 PH.configure PHconfig @17:25:34.74 PCR.off @17:25:34.78 PCR.pidtemp= 50,0.1,0,300,25,25 @17:25:34.81 PCR.caltemp= 2.7073,0.0373,0 @17:25:34.85 All dwarf controllers running firmware version 3.75 @17:25:34.97 Valve.reset @17:25:35.14 PCR.off @17:25:35.18 PCR.pidtemp= 50,0.1,0,300,25,25 @17:25:35.22 PCR.caltemp= 2.7073,0.0373,0 @17:25:35.38 Intake.select :close @17:25:35.43 Exhaust.select :close @17:25:35.45 CTV.dial :air @17:25:35.57 PSV.dial :air @17:25:35.72 CSV.dial :waste1 @17:25:35.74
Forearm.reconfigure LooseForearm @17:25:35.87 SSV.dial :spit,avoiding: :exhaust @17:25:35.89
Forearm.home.seek :retract @17:25:35.92 PS.reconfigure PSconfig @17:25:36.45 PS.home.seek 0ml @17:25:36.56 CS.reconfigure CSfast @17:25:36.59 SS.reconfigure SSconfig @17:25:36.69 CS.home.seek 0ml @17:25:36.74 SS.home.seek 0ml @17:25:36.86 Hand.close @17:25:36.89
Elbow.jog 1500 @17:25:37.48 Elbow.reconfigure LooseElbow @17:25:37.54 Elbow.home.seek :clear @17:25:41.05 SE.reconfigure SEconfig @17:25:41.11 SE.home.seek :down @17:25:41.82 SC.reconfigure SCsloppy @17:25:41.88 SC.home.seek :initial @17:25:45.81 PS.coast @17:25:45.93
Forearm.seek :retract @17:25:46.01 Elbow.seek :hammer @17:25:47.22 Forearm.reconfigure TightForearm @17:25:47.28 Forearm.seek 5 + 350 counts @17:25:48.33 Forearm.seek :retract @17:25:49.54 PC.reconfigure PCconfig @17:25:49.56
CC.reconfigure CCconfig @17:25:49.65 PC.home.seek :open @17:25:49.68
CC.home.seek :open @17:25:53.02 Phase [postDeployPhase] @17:25:53.03 GoodNews.email "At 17:25:52 began in tube 4: postDeployPhase\n/home/esp/esp2/mission/18tun_postDeploy.rb:104\n{}\nCan@17:25:53, 20.7C, 39% humidity, 14.8psia, 11.957V, 0.809A, 0.802A avg, 9.67W",Subject: "Started 18tun_postDeploy phase postDeployPhase" @17:25:53.07CET19-Nov-18
==================> STARTED postDeploycoreNegNTC (phase #1) <=== @17:25:53.18 Starting mfb @17:25:53.20 pcrBAC wcr sampling at most 0ml @17:25:53.29 Loading sh1 puck from tube 4 into Collection Clamp @17:25:53.39 Readying the MFB @17:25:53.41
Hand.open @17:25:53.43 Homing MFB Syringe @17:25:53.49 AFV.select :holdingCoil @17:25:53.65 ATV.dial :air2 @17:25:54.36
SC.reconfigure SCtight @17:25:54.42 SC.seek 4 @17:25:55.17 AS.reconfigure ASconfig @17:25:55.23 AS.home.seek :home @17:25:58.04
SC.reconfigure SCloose @17:25:58.23 Elbow.reconfigure TightElbow @17:25:58.29 Elbow.seek 4 @17:25:58.84 Forearm.seek 4 + 700 counts @17:25:59.38 BACfans.email "Started mfb in tube 4 at 17:25:53CET19-Nov-18\n{}\nCan@17:25:53, 20.7C, 39% humidity, 14.8psia, 11.957V, 0.809A, 0.802A avg, 9.67W",Subject: "pcrBAC wcr sampling at most 0ml" @17:25:59.84
SE.reconfigure SEpick @17:25:59.93 SE.seek beamBreak @17:26:00.76 AFV.select :carrierSolution @17:26:00.88 delay 2 seconds @17:26:01.84
SE.configure SEconfig @17:26:02.03 Elbow.seek 4 @17:26:02.24 Forearm.seek 4 + 300 counts @17:26:02.43 Forearm.reconfigure LooseForearm @17:26:02.51 Hand.close @17:26:02.90 AS.seek 1ml @17:26:03.36 SE.seek :down @17:26:03.55
Forearm.seek :retract @17:26:04.58 Elbow.seek CC @17:26:04.88 SE.coast @17:26:06.30
Elbow.reconfigure LooseElbow @17:26:06.41 Forearm.reconfigure TightForearm @17:26:06.48 Forearm.seek CC @17:26:08.01 CC.seek :closed @17:26:12.37 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.32 @17:26:12.38 Hand.open @17:26:13.24 Forearm.reconfigure LooseForearm @17:26:13.30 Forearm.seek :retract @17:26:14.30 SC.coast @17:26:14.35 skipping prime and sample collection @17:26:14.37 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @17:26:14.39 Intake.select :close @17:26:14.49 CSV.dial :waste @17:26:14.54 CS.seek 0ml @17:26:14.68
Calibrating pressure and temperature sensors @17:26:14.70 CTV.dial :air @17:26:14.72 SSV.dial :spit,avoiding: :exhaust @17:26:14.76 SS.reconfigure SSfast @17:26:14.84 SS.seek 5ml @17:26:14.86 CSV.dial :air @17:26:15.77 CS.seek 10ml @17:26:17.67 SSV.dial CBV @17:26:17.70
CBV.dial SSV @17:26:19.24 delay 3.8 seconds @17:26:20.29 -> exit @17:26:20.31 Session Ended @17:26:23.16
SSV.dial :spit @17:26:24.50 Session Begun @17:26:24.68
SS.seek 0ml @17:26:27.67 SSV.dial CBV @17:26:29.18 SSV.dial CBV,avoiding: :exhaust @17:26:29.21
CBV.dial SSV @17:26:29.24 CTV.dial :air @17:26:29.26 delay 15 seconds @17:26:44.29 SSV.dial :spit,avoiding: :exhaust @17:26:45.81 CTV.dial CSV,avoiding: :intake @17:26:45.90
SS.reconfigure SSexhale @17:26:45.96 SS.seek 0ml @17:26:46.03 SSV.dial CBV,avoiding: :exhaust @17:26:47.62 CBV.dial between SSV and waste2 @17:26:48.06 CTV.dial CSV,avoiding: :air @17:26:48.10
CSV.dial CTV @17:26:48.63 CS.reconfigure CSpressurize @17:26:48.70 CS.seek 0ml @17:26:55.81 delay 1 second @17:26:56.83 AFV.select :holdingCoil @17:26:56.94 delay 1 second @17:26:57.95 ATV.dial :waste @17:26:59.01 AS.reconfigure ASnorm @17:26:59.07 AS.home.seek :empty @17:27:15.10
CSV.dial :air @17:27:15.64 CS.reconfigure CSfast @17:27:15.70 CS.seek 10ml @17:27:42.44 CSV.dial between air and CTV @17:27:42.81 CS.seek 3.424ml @17:27:50.29 ATV.dial :waste @17:27:50.30 ARV.dial between carrier and air1 @17:27:50.40 ASV.dial :waste1 @17:28:00.63 CTV.dial CSV,avoiding: :air @17:28:00.66
CSV.dial CTV @17:28:01.05 CS.reconfigure CSpressurize @17:28:01.11 CS.seek 0ml @17:28:10.63 CSV.dial :air @17:28:11.18 CS.reconfigure CSfast @17:28:11.25 CS.seek 10ml @17:28:37.88 CSV.dial between air and CTV @17:28:38.25 CS.seek 2.156ml @17:28:59.42 CTV.dial CSV,avoiding: :air @17:28:59.45
CSV.dial CTV @17:28:59.82 CS.reconfigure CSpressurize @17:28:59.88 CS.seek 0ml @17:29:01.58 BubblePoint: Intake @69.8psia, Outlet @14.6psia @17:29:01.59 CBV.dial SSV @17:29:02.39 SS.reconfigure SSconfig @17:29:02.45 SS.seek 10ml @17:29:08.53 delay 15 seconds @17:29:23.65 Evacuated: Intake @29.0psia, Outlet @29.1psia @17:29:23.66 SSV.dial :spit,avoiding: :exhaust @17:29:25.21 SS.reconfigure SSexhale @17:29:25.27 SS.seek 0ml @17:29:30.17 SS.reconfigure SSfast @17:29:30.24 SS.seek 5ml @17:29:33.26 SSV.dial CBV,avoiding: :exhaust @17:29:33.29
CBV.dial SSV @17:29:34.83 CTV.dial :air,avoiding: :intake @17:29:36.35 CSV.dial CTV @17:29:36.40
SS.seek 10ml @17:29:37.38 CSV.dial :air @17:29:37.91 CS.reconfigure CSfast @17:29:37.96 CS.seek 0ml @17:29:39.18
SSV.dial :spit,avoiding: :exhaust @17:29:40.71 SS.reconfigure SSexhale @17:29:40.77 SS.seek 0ml @17:29:43.42 CS.coast @17:29:46.00
SS.reconfigure SSfast @17:29:46.06 SS.seek 5ml @17:29:48.89 SSV.dial CBV,avoiding: :exhaust @17:29:48.92
CBV.dial SSV @17:29:51.46 SSV.dial :spit,avoiding: :exhaust @17:29:52.99 SS.reconfigure SSconfig @17:29:53.05 SS.seek 0ml @17:29:56.09 SS.coast @17:29:56.14 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @17:29:56.15 Intake.select :close @17:29:56.25 CSV.dial :waste @17:29:56.36 SSV.dial CBV,avoiding: :exhaust @17:29:56.39
CBV.dial SSV @17:29:57.37 CS.seek 0ml @17:29:57.45 CSV.dial :air @17:29:57.90
CTV.dial :air @17:29:57.92 delay 15 seconds @17:29:58.32 CS.seek 10ml @17:30:12.94
SSV.dial :spit,avoiding: :exhaust @17:30:14.45 CTV.dial CSV,avoiding: :intake @17:30:14.50
SS.reconfigure SSexhale @17:30:14.60 SS.seek 0ml @17:30:14.67 SSV.dial CBV,avoiding: :exhaust @17:30:25.20 CBV.dial between SSV and waste2 @17:30:25.63 CTV.dial CSV,avoiding: :air @17:30:25.67
CSV.dial CTV @17:30:26.19 CS.reconfigure CSpressurize @17:30:26.25 CS.seek 0ml @17:30:52.42 CSV.dial :air @17:30:52.96 CS.reconfigure CSfast @17:30:53.02 CS.seek 10ml @17:31:19.63 CSV.dial between air and CTV @17:31:20.00 CS.seek 3.41ml @17:31:37.76 CTV.dial CSV,avoiding: :air @17:31:37.80
CSV.dial CTV @17:31:38.15 CS.reconfigure CSpressurize @17:31:38.21 CS.seek 0ml @17:31:47.70 CSV.dial :air @17:31:48.24 CS.reconfigure CSfast @17:31:48.30 CS.seek 10ml @17:32:14.95 CSV.dial between air and CTV @17:32:15.32 CS.seek 2.156ml @17:32:36.32 CTV.dial CSV,avoiding: :air @17:32:36.36
CSV.dial CTV @17:32:36.71 CS.reconfigure CSpressurize @17:32:36.77 CS.seek 0ml @17:32:38.58 BubblePoint: Intake @70.3psia, Outlet @15.1psia CBV.dial SSV @17:32:39.31 SS.reconfigure SSconfig @17:32:39.43 SS.seek 10ml @17:32:45.49 delay 15 seconds @17:33:00.61 Evacuated: Intake @28.8psia, Outlet @29.0psia @17:33:00.62 SSV.dial :spit,avoiding: :exhaust @17:33:02.16 SS.reconfigure SSexhale @17:33:02.22 SS.seek 0ml @17:33:07.14 SS.reconfigure SSfast @17:33:07.21 SS.seek 5ml @17:33:10.06 SSV.dial CBV,avoiding: :exhaust @17:33:10.09
CBV.dial SSV @17:33:11.60 CTV.dial :air,avoiding: :intake @17:33:13.14 CSV.dial CTV @17:33:13.19
SS.seek 10ml @17:33:14.17 CSV.dial :air @17:33:14.70 CS.reconfigure CSfast @17:33:14.76 CS.seek 0ml @17:33:15.99
SSV.dial :spit,avoiding: :exhaust @17:33:17.50 SS.reconfigure SSexhale @17:33:17.57 SS.seek 0ml @17:33:20.11 CS.coast @17:33:22.83
SS.reconfigure SSfast @17:33:22.91 SS.seek 5ml @17:33:25.91 SSV.dial CBV,avoiding: :exhaust @17:33:25.93
CBV.dial SSV @17:33:28.46 SSV.dial :spit,avoiding: :exhaust @17:33:29.97 SS.reconfigure SSconfig @17:33:30.03 SS.seek 0ml @17:33:33.10 SS.coast @17:33:33.15 Lysing: Adding 1.5ml lysis @17:33:33.16 CBV.dial between CSV and waste1,avoiding: :DBV @17:33:34.89 CTV.dial CSV @17:33:36.42 CSV.dial CSR @17:33:37.33 Pulling 1ml of CSR.lysis @17:33:37.34 CSR.select :lysis @17:33:37.48 CS.reconfigure CSconfig @17:33:37.55 CS.seek 1ml @17:33:41.17 delay 5 seconds @17:33:46.19 CSR.select :air @17:33:46.37 CS.seek 6ml @17:34:00.20 delay 2 seconds @17:34:02.22 CSV.dial :waste1 @17:34:03.71 CS.reconfigure CSfast @17:34:03.77 CS.seek :empty @17:34:19.98 CSV.dial CSR @17:34:21.54 Pulling 1.5ml of CSR.lysis @17:34:21.55 CSR.select :lysis @17:34:21.68 CS.reconfigure CSconfig @17:34:21.75 CS.seek 1.5ml @17:34:26.61 delay 5 seconds @17:34:31.63 CSR.select :air @17:34:31.81 CS.seek 4.5ml @17:34:40.45 delay 2 seconds @17:34:42.47 CSV.dial CTV @17:34:42.99 CS.seek 1.275ml @17:34:52.24 Heating sh1 puck from tube 4 in Collection Clamp to 85C for 5:00 @17:34:52.26 CH.seek 85C,8:00 @17:38:09.89 CH.hold 5:00,85C @17:43:10.05 Collection Puck Heater at 85.1C after lysing @17:43:10.07 CS.seek 0ml @17:43:14.58 CTV.dial between spare and air,avoiding: :intake @17:43:15.77 CSV.dial :air @17:43:16.31 CS.seek 5ml @17:43:30.30 CSV.dial CTV @17:43:30.83 delay 2 seconds @17:43:32.85 CSV.dial :air @17:43:33.38 delay 2 seconds @17:43:35.40 CSV.dial CTV @17:43:35.92 delay 2 seconds @17:43:37.94 CSV.dial :air @17:43:38.47 delay 2 seconds @17:43:40.50 CS.seek 0ml @17:43:54.21 Recovering Lysate into Collection syringe @17:43:54.22 CSV.dial CBV @17:43:55.42 CBV.dial CSV @17:43:55.86 CS.seek 5ml @17:44:09.67 delay 5 seconds @17:44:14.69 CTV.dial :air @17:44:15.28 CS.seek 7ml @17:44:21.42 delay 5 seconds @17:44:26.44 CSV.dial :air2 @17:44:26.97 CS.seek 10ml @17:44:35.67 CSV.dial between air and CTV,avoiding: CBV @17:44:37.00 Lysing Complete @17:44:37.09 Storing sh1 puck in Collection Clamp to tube 3 @17:44:37.26 SC.reconfigure SCtight @17:44:37.32 SC.seek 3 @17:44:39.43 SC.reconfigure SCloose @17:44:39.64 Elbow.reconfigure TightElbow @17:44:39.70 Elbow.seek 3 @17:44:41.62 Forearm.reconfigure TightForearm @17:44:41.68 Forearm.seek 3 + 700 counts @17:44:42.73 SE.reconfigure SEpick @17:44:42.82 SE.seek beamBreak @17:44:45.84 SE.configure SEconfig @17:44:45.91 SE.seek :place @17:44:46.52 Forearm.reconfigure LooseForearm @17:44:46.58 Forearm.seek :retract @17:44:47.48 Elbow.seek CC @17:44:49.10 Elbow.reconfigure LooseElbow @17:44:49.21 Forearm.seek CC @17:44:50.23 Hand.close @17:44:51.33 CC.seek :open @17:44:55.89 Forearm.seek :retract @17:44:56.92 Elbow.reconfigure TightElbow @17:44:56.99 Elbow.seek 3 @17:44:59.01 Elbow.reconfigure LimpElbow @17:44:59.08 Forearm.seek 3 @17:45:00.14 Elbow.hold @17:45:00.23 Elbow.reconfigure TightElbow @17:45:00.29 Hand.open @17:45:01.21 Forearm.force -20 @17:45:01.26 Forearm.seek 3 + 347 counts @17:45:01.59 Forearm.reconfigure TightForearm @17:45:01.65 Forearm.seek 3 + 700 counts @17:45:02.05 SE.seek :down @17:45:03.19
Forearm.reconfigure LooseForearm @17:45:03.26 Forearm.seek :retract @17:45:04.97 SE.coast @17:45:05.02
SC.coast @17:45:05.07 Tranferring lysate to PRV @17:45:05.08 CSV.dial :air @17:45:05.44 CS.seek 10ml @17:45:05.52 CSV.dial between air and CTV @17:45:05.88 AIV.dial :bypass @17:45:05.97 Beginning samplePassC @17:45:05.99 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:45:06.08 Hand.open @17:45:06.45 Elbow.seek FlushPuck::Garage @17:45:08.45 Elbow.reconfigure LooseElbow @17:45:08.56 Forearm.seek :garage @17:45:09.61 Hand.close @17:45:10.45 Forearm.seek :retract @17:45:11.48 Elbow.reconfigure TightElbow @17:45:11.55 Elbow.seek CC @17:45:14.45 Elbow.reconfigure LooseElbow @17:45:14.56 Forearm.reconfigure TightForearm @17:45:14.61 Forearm.seek CC @17:45:15.91 CC.seek :closed @17:45:20.17 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.26 @17:45:20.25 Hand.open @17:45:21.01 Forearm.reconfigure LooseForearm @17:45:21.07 Forearm.seek :retract @17:45:22.06 AWV.select :air @17:45:22.07 AIV.dial :bypass @17:45:22.10 PSV.dial PSR @17:45:23.67 Pulling 1.5ml of PSR.flush @17:45:23.68 PSR.select :flush @17:45:23.83 PS.seek 1.5ml @17:45:40.25 delay 5 seconds @17:45:45.27 PSV.dial :waste1 @17:45:45.81 PS.seek 0ml @17:46:02.10 PSV.dial PSR @17:46:02.63 PSR.select :air @17:46:03.22 PS.seek 2.5ml @17:46:29.85 PRV.dial CBV @17:46:29.88
CBV.dial PRV @17:46:30.80 PSV.dial :mixloop @17:46:31.96 CTV.dial :air,avoiding: :intake @17:46:31.98 PTV.dial :PRVmixing @17:46:32.09 PS.seek 0ml @17:46:58.91 PSV.dial :air @17:46:59.44 PS.seek 2.5ml @17:47:26.04 PSV.dial :mixloop @17:47:26.55 PS.seek 0ml @17:47:53.22 PSV.dial :air @17:47:53.73 PS.seek 2.5ml @17:48:20.35 PSV.dial :mixloop @17:48:20.86 PS.seek 0ml @17:48:47.47 Garaging FlushPuck in Collection Clamp @17:48:47.56 Elbow.reconfigure TightElbow @17:48:47.62 Elbow.seek CC @17:48:47.77 Elbow.reconfigure LooseElbow @17:48:47.88 Forearm.seek CC @17:48:48.88 Hand.close @17:48:49.59 CC.seek :open @17:48:53.98 Forearm.seek :retract @17:48:54.97 Elbow.reconfigure TightElbow @17:48:55.03 Elbow.seek :garage @17:48:58.14 Elbow.reconfigure LooseElbow @17:48:58.24 Forearm.reconfigure TightForearm @17:48:58.31 Forearm.seek :garage @17:48:59.47 Hand.open @17:49:00.27 Forearm.reconfigure LooseForearm @17:49:00.33 Forearm.seek :retract @17:49:01.38 Connecting PS to CS to transfer sample @17:49:01.39 CBV.dial AIV @17:49:01.42 AIV.dial :bypass @17:49:01.44 PSV.dial :mixloop @17:49:01.47 PTV.dial :PRVmixing @17:49:01.49 PRV.dial AIV @17:49:01.51 CTV.dial :air,avoiding: :intake @17:49:01.54 Loading lyfil puck from tube 4 into Collection Clamp @17:49:01.78 SC.reconfigure SCtight @17:49:01.84 SC.seek 4 @17:49:03.94 SC.reconfigure SCloose @17:49:04.16 Elbow.reconfigure TightElbow @17:49:04.22 Elbow.seek 4 @17:49:06.28 Forearm.reconfigure TightForearm @17:49:06.34 Forearm.seek 4 + 700 counts @17:49:07.39 SE.reconfigure SEpick @17:49:07.48 SE.seek beamBreak @17:49:09.40 SE.configure SEconfig @17:49:09.56 Elbow.seek 4 @17:49:09.74 Forearm.seek 4 + 300 counts @17:49:10.02 Forearm.reconfigure LooseForearm @17:49:10.08 Hand.close @17:49:10.85 SE.seek :down @17:49:11.04
Forearm.seek :retract @17:49:12.00 Elbow.seek CC @17:49:12.49 SE.coast @17:49:13.66
Elbow.reconfigure LooseElbow @17:49:13.77 Forearm.reconfigure TightForearm @17:49:13.83 Forearm.seek CC @17:49:15.03 CC.seek :closed @17:49:19.29 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.27 @17:49:19.30 Hand.open @17:49:20.14 Forearm.reconfigure LooseForearm @17:49:20.20 Forearm.seek :retract @17:49:21.19 SC.coast @17:49:21.24 CTV.dial CSV,avoiding: :intake @17:49:22.76 CSV.dial CTV @17:49:23.11 CS.seek 5ml @17:49:36.96 delay 1:20 @17:50:57.00 CS.seek 2.5ml @17:51:04.37 delay 40 seconds @17:51:44.40 CS.seek 0ml @17:51:51.80 delay 1:00 @17:52:51.83 PSV.dial :air @17:52:52.36 PS.seek 2.5ml @17:53:18.91 PSV.dial :mixloop @17:53:19.43 delay 3 seconds @17:53:22.45 PSV.dial :air @17:53:22.96 delay 2 seconds @17:53:24.98 PSV.dial :mixloop @17:53:25.48 delay 3 seconds @17:53:28.50 PSV.dial :waste1 @17:53:30.01 PS.seek :empty @17:53:56.73 CBV.dial :waste1 @17:53:57.26 delay 5 seconds @17:54:02.28 CBV.dial AIV @17:54:02.82 delay 10 seconds @17:54:12.85 CBV.dial :waste1 @17:54:13.37 delay 10 seconds @17:54:23.40 CBV.dial AIV @17:54:23.93 delay 10 seconds @17:54:33.96 CTV.dial CSV,avoiding: :intake @17:54:33.98 CBV.dial between AIV and waste1 @17:54:34.37 CSV.dial :air @17:54:34.91 CS.seek 10ml @17:55:01.40 CSV.dial CTV @17:55:01.94 CS.seek 0ml @17:55:28.87 CBV.dial :waste1 @17:55:29.21 delay 10 seconds @17:55:39.24 CTV.dial :air,avoiding: :intake @17:55:40.80 Storing lyfil puck in Collection Clamp to tube 3 @17:55:40.98 SC.reconfigure SCtight @17:55:41.04 SC.seek 3 @17:55:43.17 SC.reconfigure SCloose @17:55:43.38 Elbow.reconfigure TightElbow @17:55:43.44 Elbow.seek 3 @17:55:45.34 Forearm.reconfigure TightForearm @17:55:45.41 Forearm.seek 3 + 700 counts @17:55:46.45 SE.reconfigure SEpick @17:55:46.54 SE.seek beamBreak @17:55:49.65 SE.configure SEconfig @17:55:49.81 Elbow.reconfigure LimpElbow @17:55:49.87 Forearm.reconfigure LooseForearm @17:55:49.93 Forearm.seek 3 @17:55:50.26 Elbow.hold @17:55:50.34 Elbow.reconfigure TightElbow @17:55:50.45 SE.seek 0.07 Pucks below Top Plate @17:55:50.89 Forearm.force -20 @17:55:50.94 Forearm.seek 3 + 340 counts @17:55:51.24 Forearm.reconfigure TightForearm @17:55:51.30 Forearm.seek 3 + 700 counts @17:55:51.54 SE.seek 1.07 Pucks below Top Plate @17:55:52.58 Forearm.reconfigure LooseForearm @17:55:52.64 Forearm.seek :retract @17:55:53.59 Elbow.seek CC @17:55:55.36 Elbow.reconfigure LooseElbow @17:55:55.47 Forearm.seek CC @17:55:56.45 Hand.close @17:55:57.21 CC.seek :open @17:56:01.71 Forearm.seek :retract @17:56:02.70 Elbow.reconfigure TightElbow @17:56:02.77 Elbow.seek 3 @17:56:04.61 Elbow.reconfigure LimpElbow @17:56:04.68 Forearm.seek 3 @17:56:05.71 Elbow.hold @17:56:05.79 Elbow.reconfigure TightElbow @17:56:05.85 Hand.open @17:56:06.71 Forearm.force -20 @17:56:06.76 Forearm.seek 3 + 340 counts @17:56:07.07 Forearm.reconfigure TightForearm @17:56:07.13 Forearm.seek 3 + 700 counts @17:56:07.49 SE.seek :down @17:56:08.61
Forearm.reconfigure LooseForearm @17:56:08.68 Forearm.seek :retract @17:56:10.25 SE.coast @17:56:10.30
SC.coast @17:56:10.35 PSV.dial :air @17:56:11.57 PS.seek 0.499ml @17:56:17.51 delay 3 seconds @17:56:20.53 PSV.dial :mixloop @17:56:21.04 PRV.dial :air @17:56:21.57 delay 3 seconds @17:56:24.59 PRV.dial CBV @17:56:25.12 CBV.dial AIV @17:56:25.65 PRV.dial between CBV and air @17:56:26.01 PRV.dial :air @17:56:26.41 PS.reconfigure PSslow @17:56:26.47 PS.seek 0.599ml @17:56:28.95 PRV.dial :waste2 @17:56:29.78 PS.reconfigure PSconfig @17:56:29.84 PS.seek :empty @17:56:36.82 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:56:36.90 Hand.open @17:56:37.20 Elbow.seek FlushPuck::Garage @17:56:39.15 Elbow.reconfigure LooseElbow @17:56:39.25 Forearm.seek :garage @17:56:40.31 Hand.close @17:56:41.21 Forearm.seek :retract @17:56:42.29 Elbow.reconfigure TightElbow @17:56:42.35 Elbow.seek CC @17:56:45.24 Elbow.reconfigure LooseElbow @17:56:45.34 Forearm.reconfigure TightForearm @17:56:45.40 Forearm.seek CC @17:56:47.11 CC.seek :closed @17:56:51.36 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.28 @17:56:51.45 Hand.open @17:56:52.28 Forearm.reconfigure LooseForearm @17:56:52.35 Forearm.seek :retract @17:56:53.38 Clearing PML for modified lysate @17:56:53.39 CTV.dial :air,avoiding: :intake @17:56:53.42 PRV.dial :waste2 @17:56:53.45 PTV.dial :PRV,avoiding: :puck @17:56:54.29 delay 2 seconds @17:56:56.31 PTV.dial :PRVmixing,avoiding: :puck @17:56:57.15 delay 2 seconds @17:56:59.17 PSV.dial :PML @17:56:59.19 delay 2 seconds @17:57:01.21 PRV.dial :air @17:57:02.05 delay 2 seconds @17:57:04.13 PS.seek 1.499ml @17:57:20.41 delay 5 seconds @17:57:25.44 PS.seek 2.5ml @17:57:36.51 PSV.dial :air @17:57:37.03 PS.seek 0ml @17:58:03.66 mixing lysate with speDil @17:58:03.67 PSV.dial :PML @17:58:04.20 CBV.dial :PRV @17:58:04.21 PRV.dial :air @17:58:04.24 delay 2 seconds @17:58:06.27 PRV.dial :speDil @17:58:06.77 delay 2 seconds @17:58:08.86 PS.reconfigure PSslow1 @17:58:08.92 PS.seek 0.225ml @17:58:32.79 delay 5 seconds @17:58:37.81 PRV.dial CBV @17:58:38.68 delay 2 seconds @17:58:40.76 PS.seek 0.475ml @17:59:06.48 delay 5 seconds @17:59:11.50 PRV.dial :air @17:59:12.04 delay 2 seconds @17:59:14.07 PS.seek 0.965ml @18:00:04.29 delay 10 seconds @18:00:14.32 PRV.dial CBV @18:00:14.85 CTV.dial :air,avoiding: :intake @18:00:14.86 PSV.dial :air @18:00:15.41 PS.reconfigure PSconfig @18:00:15.47 PS.seek 2.5ml @18:00:32.20 delay 3 seconds @18:00:35.22 PSV.dial PTV @18:00:36.07 PTV.dial :PRV,avoiding: :puck @18:00:36.93 PS.seek 0ml @18:01:03.63 PSV.dial :air @18:01:04.49 PS.seek 2.5ml @18:01:31.10 delay 3 seconds @18:01:34.12 PSV.dial PTV @18:01:34.96 PTV.dial :PRV,avoiding: :puck @18:01:34.99 PS.seek 0ml @18:02:01.67 Pushing modified lysate into AIV Injection Loop @18:02:01.68 CBV.dial AIV @18:02:01.71 AIV.dial :bypass @18:02:01.73 PRV.dial CBV @18:02:01.75 PSV.dial :air @18:02:02.63 PS.seek 2.5ml @18:02:29.26 delay 3 seconds @18:02:32.28 PSV.dial :PML @18:02:32.79 PTV.dial :PRVmixing,avoiding: :puck @18:02:33.70 PS.reconfigure PSslow1 @18:02:33.76 PS.seek 1.74ml @18:03:51.57 delay 5 seconds @18:03:56.59 AIV.dial :sample @18:03:57.25 AWV.select :waste @18:03:57.43 PS.reconfigure PSslow2 @18:03:57.49 PS.seek 1.22ml @18:05:44.82 delay 15 seconds @18:05:59.84 AIV.dial :bypass @18:06:00.49 PS.reconfigure PSconfig @18:06:00.55 PS.seek 0ml @18:06:13.86 PSV.dial :air @18:06:14.38 PS.seek 2.5ml @18:06:40.73 PSV.dial :PML @18:06:41.26 PS.seek 0ml @18:07:07.98 AIV.dial :coilToMFB @18:07:07.99 AWV.select :air @18:07:08.18 Extracting Nucleic Acid from AIV @18:07:08.20
Beginning samplePassD @18:07:08.24 SPE.hold 2:46:40,55C @18:07:08.33 ASV.dial :SPEdelivery @18:07:08.42
AWV.select :air @18:07:08.43 AIV.dial :bypass @18:07:08.46 PSV.dial PSR @18:07:08.79 delay 20 seconds @18:07:09.77
Pulling 1.5ml of PSR.killMFB PSR.select :killMFB @18:07:09.93 PS.seek 1.5ml @18:07:26.86 delay 5 seconds @18:07:28.81 primeLysateINT @18:07:28.82 AWV.select :air @18:07:28.84 AIV.dial :coilToMFB @18:07:28.87 ATV.dial AIV @18:07:30.76 AS.reconfigure ASslow1 @18:07:30.82 AS.seek 0.299ml @18:07:31.88
PSV.dial :waste1 @18:07:32.44 PS.seek 0ml @18:07:49.45 PSV.dial PSR @18:07:50.00 PSR.select :air @18:07:50.13 PS.seek 2.5ml @18:08:17.86 PRV.dial CBV @18:08:17.88
CBV.dial PRV @18:08:17.91 PSV.dial :mixloop @18:08:19.15 CTV.dial :air,avoiding: :intake @18:08:19.16 PTV.dial :PRVmixing @18:08:19.20 PS.seek 0ml @18:08:31.45 delay 10 seconds @18:08:41.48 ATV.dial :air2 @18:08:42.37 AS.seek 0.329ml @18:08:47.29
PSV.dial PSR @18:08:48.59 Pulling 1.5ml of PSR.flush PSR.select :flush @18:08:48.70 ATV.dial :waste2 @18:08:48.77
PS.seek 1.5ml @18:08:49.30 AS.reconfigure ASnorm @18:08:49.36 AS.seek :empty @18:09:05.83
delay 5 seconds @18:09:06.37 Lysate primed! @18:09:06.38 AFV.select :holdingCoil @18:09:06.40 ATV.dial :air2 @18:09:07.07 AS.reconfigure ASslow1 @18:09:07.13 AS.seek 0.069ml @18:09:10.85
PSV.dial :waste1 @18:09:11.41 PS.seek 0ml @18:09:21.59 delay 3 seconds @18:09:24.61 ATV.dial :AIV @18:09:25.51 AS.seek 0.469ml @18:09:28.42
PSV.dial PSR @18:09:28.97 PSR.select :air @18:09:29.10 PS.seek 2.5ml @18:09:56.89 PRV.dial CBV @18:09:56.93
CBV.dial PRV @18:09:56.94 PSV.dial :mixloop @18:09:58.15 CTV.dial :air,avoiding: :intake @18:09:58.16 PTV.dial :PRVmixing @18:09:58.20 PS.seek 0ml @18:10:25.79 PSV.dial PSR @18:10:27.06 Pulling 1.5ml of PSR.flush PSR.select :flush @18:10:27.21 PS.seek 1.5ml @18:10:44.45 delay 5 seconds @18:10:46.13 delay 5 seconds @18:10:49.47
PSV.dial :waste1 @18:10:50.02 PS.seek 0ml @18:10:51.20 Solid Phase Extraction Heater at 49C @18:10:51.21 ATV.dial ASV @18:10:51.63 ASV.dial :SPEdelivery @18:10:51.72 AS.seek 0.041ml @18:11:06.86
PSV.dial PSR @18:11:07.40 PSR.select :air @18:11:07.54 PS.seek 2.5ml @18:11:34.67 PRV.dial CBV @18:11:34.71
CBV.dial PRV @18:11:34.73 PSV.dial :mixloop @18:11:35.92 CTV.dial :air,avoiding: :intake @18:11:35.93 PTV.dial :PRVmixing @18:11:35.97 PS.seek 0ml @18:12:03.14 PSV.dial PSR @18:12:04.39 Pulling 1.5ml of PSR.flush PSR.select :flush @18:12:04.55 PS.seek 1.5ml @18:12:18.07 delay 10 seconds @18:12:21.01
delay 5 seconds @18:12:26.03 PSV.dial :waste1 @18:12:26.56 PS.seek 0ml @18:12:28.10 rinseLysateLineINT @18:12:28.11 AFV.select :carrierSolution @18:12:28.24 delay 2 seconds @18:12:30.26 AS.reconfigure ASconfig @18:12:30.31 AS.seek 1ml @18:12:43.08
PSV.dial PSR @18:12:43.61 PSR.select :air @18:12:43.74 PS.seek 2.5ml @18:13:10.45 PRV.dial CBV @18:13:10.49
CBV.dial PRV @18:13:10.51 PSV.dial :mixloop @18:13:11.81 CTV.dial :air,avoiding: :intake @18:13:11.82 PTV.dial :PRVmixing @18:13:11.86 PS.reconfigure PSslow @18:13:11.93 PS.seek 1.2ml @18:13:25.71 delay 1 second @18:13:26.73 AFV.select :holdingCoil @18:13:26.84 delay 1 second @18:13:27.85 ATV.dial AIV @18:13:28.52 AIV.dial :coilToMFB @18:13:28.53 AWV.select :waste @18:13:28.67 AS.reconfigure ASslow0 @18:13:28.73 AS.seek 0ml @18:13:38.76
PSV.dial :waste1 @18:13:40.27 PS.reconfigure PSconfig @18:13:40.33 PS.seek 0ml @18:13:53.67 PSV.dial :air @18:13:54.85 PS.seek 2.5ml @18:14:21.76 PSV.dial :mixloop @18:14:22.27 PS.reconfigure PSslow @18:14:22.33 PS.seek 0ml @18:15:09.60 ATV.dial :air2 @18:15:10.29 AS.reconfigure ASslow1 @18:15:10.35 AS.seek 0.05ml @18:15:13.36
PSV.dial :air @18:15:13.88 PS.reconfigure PSconfig @18:15:13.94 PS.seek 2.5ml @18:15:20.70 ATV.dial :AIV @18:15:21.63 AS.seek 0.01ml @18:15:29.96 ATV.dial :waste @18:15:31.71 AS.seek :empty @18:15:34.15 ethanol wash @18:15:34.16 ATV.dial :air2 @18:15:35.44 AS.reconfigure ASslow2 @18:15:35.50 AS.seek 0.009ml @18:15:40.56
PSV.dial :mixloop @18:15:41.09 PS.reconfigure PSslow @18:15:41.15 PS.seek 0ml @18:15:45.97 delay 3 seconds @18:15:49.00 Pulling 0.4ml of Ethanol Wash ATV.dial :ethanol @18:15:49.85 AS.reconfigure ASslow1 @18:15:49.91 AS.seek 0.409ml @18:16:32.30
Cleaning Collection Syringe @18:16:32.31 CSV.dial CSR @18:16:33.34 Pulling 3.999ml of CSR.flush @18:16:33.35 CSR.select :flush @18:16:33.50 CS.seek 4ml @18:16:44.76 delay 5 seconds @18:16:49.78 CSR.select :air @18:16:49.92 CS.seek 10ml @18:17:06.29 CSV.dial CTV @18:17:06.32
CTV.dial CSV @18:17:07.90 CBV.dial :waste1 @18:17:08.44 CS.seek 0ml @18:17:10.70 delay 3 seconds @18:17:13.72 ATV.dial :air1 @18:17:14.12 delay 3 seconds @18:17:17.20 AS.seek 0.434ml @18:17:22.52 delay 3 seconds @18:17:25.54 ATV.dial :ethanol @18:17:26.16 AS.seek 0.419ml @18:17:29.41 delay 3 seconds @18:17:32.43 ATV.dial ASV @18:17:33.79 AS.seek 0.092ml @18:17:34.92
CSV.dial CSR @18:17:35.51 Pulling 4ml of CSR.flush @18:17:35.52 CSR.select :flush @18:17:35.67 CS.seek 4ml @18:17:46.95 delay 5 seconds @18:17:51.97 CSR.select :air @18:17:52.11 CS.seek 10ml @18:18:08.48 CSV.dial CTV @18:18:08.51
CTV.dial CSV @18:18:09.04 CBV.dial :waste1 @18:18:09.06 CS.seek 0ml @18:18:35.69 Garaging FlushPuck in Collection Clamp @18:18:35.78 Elbow.reconfigure TightElbow @18:18:35.84 Elbow.seek CC @18:18:35.97 Elbow.reconfigure LooseElbow @18:18:36.08 Forearm.seek CC @18:18:37.05 Hand.close @18:18:37.88 CC.seek :open @18:18:39.81 delay 3 seconds @18:18:42.28
Forearm.seek :retract @18:18:42.83 ATV.dial :waste2 @18:18:43.27
Elbow.reconfigure TightElbow @18:18:43.34 Elbow.seek :garage @18:18:43.71 AS.reconfigure ASconfig @18:18:43.76 AS.seek :empty @18:18:46.42
Elbow.reconfigure LooseElbow @18:18:46.53 Forearm.reconfigure TightForearm @18:18:46.59 Forearm.seek :garage @18:18:47.79 Hand.open @18:18:48.55 Forearm.reconfigure LooseForearm @18:18:48.61 Forearm.seek :retract @18:18:49.38 ATV.dial :air2 @18:18:49.69 Whole Cell Archive sampling 0ml @18:18:49.71 Loading wcr puck from tube 4 into Collection Clamp @18:18:49.87 AS.reconfigure ASslow1 @18:18:49.94 AS.seek 1ml @18:18:50.12 SC.reconfigure SCtight @18:18:50.18 SC.seek 4 @18:18:52.30 SC.reconfigure SCloose @18:18:52.52 Elbow.reconfigure TightElbow @18:18:52.59 Elbow.seek 4 @18:18:54.63 Forearm.reconfigure TightForearm @18:18:54.69 Forearm.seek 4 + 700 counts @18:18:56.32 SE.reconfigure SEpick @18:18:56.41 SE.seek beamBreak @18:18:58.43 SE.configure SEconfig @18:18:58.61 Elbow.seek 4 @18:18:58.81 Forearm.seek 4 + 300 counts @18:18:59.07 Forearm.reconfigure LooseForearm @18:18:59.13 Hand.close @18:18:59.90 SE.seek :down @18:19:00.10 Forearm.seek :retract @18:19:01.09 Elbow.seek CC @18:19:01.57 SE.coast @18:19:02.80 Elbow.reconfigure LooseElbow @18:19:02.91 Forearm.reconfigure TightForearm @18:19:02.97 Forearm.seek CC @18:19:04.29 CC.seek :closed @18:19:08.58 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.38 @18:19:08.59 Hand.open @18:19:09.30 Forearm.reconfigure LooseForearm @18:19:09.36 Forearm.seek :retract @18:19:10.36 SC.coast @18:19:10.41 collectSample 0ml w/bubble point of 55psi @18:19:10.43 Priming 7ml @18:19:10.44 Intake.select :close @18:19:10.46 Exhaust.select :close @18:19:10.49 SS.hold @18:19:10.71 Calibrating pressure sensors @18:19:10.72 CTV.dial :air @18:19:12.27 SSV.dial :spit,avoiding: :exhaust @18:19:12.29 SS.reconfigure SSfast @18:19:12.37 SS.seek 5ml @18:19:15.15 SSV.dial CBV @18:19:15.18 CBV.dial SSV @18:19:16.79 delay 3.8 seconds @18:19:20.71 SSV.dial :spit @18:19:22.25 SS.seek 0ml @18:19:25.00 SSV.dial CBV @18:19:26.53 CTV.dial :intake @18:19:27.40 SSV.dial CBV @18:19:27.43 CBV.dial SSV @18:19:27.46 Allowing 40s for 7.0ml of remaining 7.0ml @18:19:27.49 Intake.select :open @18:19:29.67 Intake @15.1psia, vacuum from 8.6 to 12.6psi @18:19:29.69 SS.reconfigure SSinhale.with maxSpeed: 150 @18:19:29.77 SS.seek 7ml @18:19:35.67 Stroke completed -- waiting for pressure to equalize ... @18:19:36.34 Intake.select :close @18:19:36.50 SSV.dial :exhaust @18:19:37.37 Exhaust.select :open @18:19:37.50 SS.reconfigure SSexhale @18:19:37.56 SS.seek 0ml @18:19:41.89 Exhaust.select :close @18:19:42.02 Sampling 0.0ml @18:19:42.03 Intake.select :close @18:19:42.05 Exhaust.select :close @18:19:42.55 SS.reconfigure SSconfig @18:19:42.62 SS.hold @18:19:42.84 CTV.dial :intake @18:19:42.86 Sampled 0.0ml @18:19:42.88 Drying puck before fixing for 4:00 @18:19:42.90 CBV.dial :waste2b @18:19:43.79 delay 1:00 @18:20:43.82 CSV.dial :air @18:20:44.35 CS.reconfigure CSfast @18:20:44.42 CS.seek 10ml @18:21:11.13 Pumping wcr puck from tube 4 up to 55psi... @18:21:11.15 CBV.dial SSV @18:21:12.60 CBV.dial between CSV and waste2b @18:21:13.71 CSV.dial between air and CTV @18:21:14.08 CS.seek 7.357ml @18:21:21.85 CTV.dial CSV,avoiding: :air @18:21:21.88 CSV.dial CTV @18:21:22.79 CS.reconfigure CSpressurize @18:21:22.86 CS.seek 0ml @18:21:42.39 CSV.dial :air @18:21:42.94 CS.reconfigure CSfast @18:21:43.00 CS.seek 10ml @18:22:09.60 CSV.dial between air and CTV @18:22:09.97 CS.seek 2.649ml @18:22:11.70 delay 5 seconds @18:22:16.72 ATV.dial ASV @18:22:17.73 ASV.dial :SPEdelivery @18:22:17.75 AS.seek 0.02ml @18:22:29.89 CTV.dial CSV,avoiding: :air @18:22:29.92 CSV.dial CTV @18:22:30.30 CS.reconfigure CSpressurize @18:22:30.37 CS.seek 0ml @18:22:34.84 CBV.dial :waste2b @18:22:35.25 CS.reconfigure CSconfig @18:22:35.31 CS.seek :empty @18:22:39.32 delay 1:00 @18:23:39.35 CTV.dial :air,avoiding: :intake @18:23:40.88 Fix #1 of 2 (with rnal) for 2:00 @18:23:40.89 CSV.dial CSR @18:23:41.46 Pulling 2ml of CSR.rnal @18:23:41.47 CSR.select :rnal @18:23:41.62 CS.seek 2ml @18:23:47.82 delay 5 seconds @18:23:52.84 CSR.select :air @18:23:52.98 CS.reconfigure CSfast @18:23:53.04 CS.seek 10ml @18:24:14.42 Pumping wcr puck from tube 4 up to 55psi... @18:24:14.44 CBV.dial SSV @18:24:15.87 CBV.dial between CSV and waste2b @18:24:16.98 CSV.dial between air and CTV @18:24:17.69 CS.seek 7.279ml @18:24:25.67 CTV.dial CSV,avoiding: :air @18:24:25.70 CSV.dial CTV @18:24:27.26 CS.reconfigure CSpressurize @18:24:27.32 CS.seek 0ml @18:24:45.74 delay 2:00 @18:25:35.12 delay 5 seconds @18:25:40.14 ATV.dial :air2 @18:25:41.21 AS.seek 0.52ml @18:26:45.75 CBV.dial :waste2b @18:26:46.14 CS.reconfigure CSconfig @18:26:46.21 CS.seek :empty @18:26:48.08 delay 1:00 @18:27:22.06 delay 5 seconds @18:27:27.08 ATV.dial ASV @18:27:28.09 ASV.dial :wasteR @18:27:28.47 AS.seek 0.3ml @18:27:48.11 CTV.dial :air,avoiding: :intake @18:27:49.65 Fix #2 of 2 (with rnal) for 2:00 @18:27:49.66 CSV.dial CSR @18:27:50.25 Pulling 2ml of CSR.rnal @18:27:50.26 CSR.select :rnal @18:27:50.42 CS.seek 2ml @18:27:56.83 delay 5 seconds @18:28:01.86 CSR.select :air @18:28:02.00 CS.reconfigure CSfast @18:28:02.06 CS.seek 10ml @18:28:13.01 delay 20 seconds @18:28:23.76 Pumping wcr puck from tube 4 up to 55psi... @18:28:23.78 CBV.dial SSV @18:28:25.21 CBV.dial between CSV and waste2b @18:28:26.32 CSV.dial between air and CTV @18:28:27.15 CS.seek 7.34ml @18:28:33.04 AS.seek 0.07ml @18:28:34.92 CTV.dial CSV,avoiding: :air @18:28:34.95 CSV.dial CTV @18:28:36.52 CS.reconfigure CSpressurize @18:28:36.58 CS.seek 0ml @18:28:55.54 delay 2:00 @18:29:19.53 delay 20 seconds @18:29:39.56 ATV.dial :waste2 @18:29:40.43 AS.reconfigure ASconfig @18:29:40.49 AS.seek :empty @18:29:44.86 Elution @18:29:44.87 ATV.dial :air1 @18:29:46.02 AS.reconfigure ASslow1 @18:29:46.08 AS.seek 0.049ml @18:29:56.51 delay 3 seconds @18:29:59.53 ATV.dial :ElutionWater @18:30:00.23 AS.seek 0.109ml @18:30:12.59 delay 3 seconds @18:30:15.61 ATV.dial ASV,via: :air2 @18:30:17.74 ASV.dial :SPEelution @18:30:18.35 AFV.select :carrierSolution @18:30:18.51 AS.reconfigure ASnorm @18:30:18.57 AS.seek 0.759ml @18:30:51.67 delay 3 seconds @18:30:54.69 AFV.select :holdingCoil @18:30:54.84 AS.reconfigure ASslow1 @18:30:54.90 AS.seek 0.474ml @18:30:55.56 CBV.dial :waste2b @18:30:55.94 CS.reconfigure CSconfig @18:30:56.01 CS.seek :empty @18:30:57.42 delay 1:00 @18:31:52.53 delay 5 seconds @18:31:57.44 CTV.dial :air,avoiding: :intake @18:31:57.61 AS.reconfigure ASslow2 @18:31:57.67 AS.seek 0.439ml @18:31:59.03 Storing wcr puck in Collection Clamp to tube 3 @18:31:59.21 SC.reconfigure SCtight @18:31:59.28 SC.seek 3 @18:32:01.42 SC.reconfigure SCloose @18:32:02.08 Elbow.reconfigure TightElbow @18:32:02.16 Elbow.seek 3 @18:32:04.19 Forearm.reconfigure TightForearm @18:32:04.26 Forearm.seek 3 + 700 counts @18:32:05.29 SE.reconfigure SEpick @18:32:05.38 SE.seek beamBreak @18:32:08.57 SE.configure SEconfig @18:32:08.74 Elbow.reconfigure LimpElbow @18:32:08.81 Forearm.reconfigure LooseForearm @18:32:08.87 Forearm.seek 3 @18:32:09.20 Elbow.hold @18:32:09.29 Elbow.reconfigure TightElbow @18:32:09.40 SE.seek 1.07 Pucks below Top Plate @18:32:10.03 Forearm.force -20 @18:32:10.09 Forearm.seek 3 + 340 counts @18:32:10.42 Forearm.reconfigure TightForearm @18:32:10.49 Forearm.seek 3 + 700 counts @18:32:10.74 SE.seek 2.07 Pucks below Top Plate @18:32:11.37 Forearm.reconfigure LooseForearm @18:32:11.43 Forearm.seek :retract @18:32:12.38 Elbow.seek CC @18:32:14.08 Elbow.reconfigure LooseElbow @18:32:14.19 Forearm.seek CC @18:32:15.19 Hand.close @18:32:16.02 CC.seek :open @18:32:20.52 Forearm.seek :retract @18:32:21.52 Elbow.reconfigure TightElbow @18:32:21.59 Elbow.seek 3 @18:32:23.52 Elbow.reconfigure LimpElbow @18:32:23.59 Forearm.seek 3 @18:32:24.63 Elbow.hold @18:32:24.72 Elbow.reconfigure TightElbow @18:32:24.78 Hand.open @18:32:25.63 Forearm.force -20 @18:32:25.69 Forearm.seek 3 + 348 counts @18:32:26.00 Forearm.reconfigure TightForearm @18:32:26.06 Forearm.seek 3 + 700 counts @18:32:26.37 SE.seek :down @18:32:27.51 Forearm.reconfigure LooseForearm @18:32:27.57 Forearm.seek :retract @18:32:29.13 SE.coast @18:32:29.18 SC.coast @18:32:29.23 Starting finalPurge @18:32:29.25 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:32:29.74 Hand.open @18:32:30.13 Elbow.seek FlushPuck::Garage @18:32:32.05 Elbow.reconfigure LooseElbow @18:32:32.16 Forearm.seek :garage @18:32:33.21 Hand.close @18:32:33.30 delay 1:00 @18:32:34.00
Forearm.seek :retract @18:32:35.05 Elbow.reconfigure TightElbow @18:32:35.12 Elbow.seek CC @18:32:38.00 Elbow.reconfigure LooseElbow @18:32:38.11 Forearm.reconfigure TightForearm @18:32:38.17 Forearm.seek CC @18:32:39.37 CC.seek :closed @18:32:43.63 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.28 @18:32:43.72 Hand.open @18:32:44.44 Forearm.reconfigure LooseForearm @18:32:44.51 Forearm.seek :retract @18:32:45.57 Flushing the Collection Valve::Manifold @18:32:45.71 CSV.dial CSR @18:32:46.40 Pulling 5ml of CSR.flush @18:32:46.41 CSR.select :flush @18:32:46.55 CS.seek 5ml @18:33:00.42 delay 5 seconds @18:33:05.44 CSR.select :air @18:33:05.62 CS.reconfigure CSfast @18:33:05.68 CS.seek 7.5ml @18:33:13.02 CSV.dial :waste @18:33:14.53 CS.reconfigure CSconfig @18:33:14.60 CS.seek 0ml @18:33:33.32 pushing eluate to holding coil @18:33:33.39 AS.seek 0.279ml @18:33:34.83
Flushing lower manifold's waste1 port @18:33:34.97 CSV.dial :flush @18:33:35.51 CS.reconfigure CSfast @18:33:35.57 CS.seek 5ml @18:33:49.30 delay 5 seconds @18:33:54.32 CSV.dial :air @18:33:54.92 CS.seek 7ml @18:34:00.96 CSV.dial CTV @18:34:00.99
CTV.dial CSV @18:34:02.58 CBV.dial :waste1 @18:34:03.47 CS.seek 0ml @18:34:22.36 Flushing lower manifold's waste2b port @18:34:22.50 CSV.dial :flush @18:34:23.36 CS.seek 5ml @18:34:37.17 delay 5 seconds @18:34:42.19 CSV.dial :air @18:34:42.80 CS.seek 7ml @18:34:48.96 CSV.dial CTV @18:34:48.99
CTV.dial CSV @18:34:49.63 CBV.dial :waste2b @18:34:50.54 CS.seek 0ml @18:35:09.96 Flushing lower manifold's waste1 port @18:35:09.97 CSV.dial CSR @18:35:10.58 Pulling 4ml of CSR.mfbkill @18:35:10.59 CSR.select :mfbkill @18:35:10.73 CS.reconfigure CSconfig @18:35:10.79 CS.seek 4ml @18:35:22.02 delay 5 seconds @18:35:27.04 CSR.select :air @18:35:27.18 CS.reconfigure CSfast @18:35:27.25 CS.seek 10ml @18:35:43.55 CSV.dial CTV @18:35:43.61
CTV.dial CSV @18:35:44.10 CBV.dial :waste1 @18:35:45.00 CS.seek 0ml @18:36:11.49 Flushing lower manifold's waste2b port @18:36:11.50 CSV.dial CSR @18:36:12.09 Pulling 4ml of CSR.mfbkill @18:36:12.10 CSR.select :mfbkill @18:36:12.24 CS.reconfigure CSconfig @18:36:12.30 CS.seek 4ml @18:36:14.49 delay 30 seconds @18:36:23.61
delay 5 seconds @18:36:28.63 CSR.select :air @18:36:28.77 CS.reconfigure CSfast @18:36:28.84 CS.seek 10ml @18:36:44.51 ATV.dial :waste,via: :air3 @18:36:45.18 CSV.dial CTV @18:36:45.21
CTV.dial CSV @18:36:45.75 CBV.dial :waste2b @18:36:46.24 AS.reconfigure ASconfig @18:36:46.30 AS.seek :empty @18:36:46.64
CS.seek 0ml @18:37:02.71 Parking Bleach in LysateSource line and SPEcolumn @18:37:02.72 ATV.dial :air3 @18:37:03.57 AS.reconfigure ASslow1 @18:37:03.63 AS.seek 0.029ml @18:37:09.96 ATV.dial :ElutionWater @18:37:10.59 AS.seek 0.059ml @18:37:13.15
Killing upper manifold @18:37:13.16 CSV.dial CSR @18:37:13.77 Pulling 4ml of CSR.mfbkill @18:37:13.78 CSR.select :mfbkill @18:37:13.92 CS.reconfigure CSconfig @18:37:13.98 CS.seek 4ml @18:37:16.90 ATV.dial :air3 @18:37:17.52 AS.seek 0.069ml @18:37:19.71 delay 2 seconds @18:37:21.73 ATV.dial :bleach @18:37:22.19 AS.seek 0.769ml @18:37:25.24
delay 5 seconds @18:37:30.26 CSR.select :air @18:37:30.39 CS.reconfigure CSfast @18:37:30.46 CS.seek 10ml @18:37:46.77 CSV.dial CBV @18:37:46.80
CBV.dial CSV @18:37:48.02 CTV.dial :air @18:37:49.55 CS.seek 0ml @18:38:16.02 delay 7 seconds @18:38:23.05 CS.seek 3ml @18:38:31.80 CSV.dial :waste @18:38:32.33 CS.seek 0ml @18:38:40.93 Flushing upper manifold @18:38:41.07 CSV.dial :flush @18:38:41.58 CS.seek 5ml @18:38:55.55 delay 5 seconds @18:39:00.57 CSV.dial :air @18:39:01.14 CS.seek 7ml @18:39:07.33 CSV.dial CBV @18:39:07.35
CBV.dial CSV @18:39:08.57 CTV.dial :air @18:39:08.59 CS.seek 0ml @18:39:27.30 delay 7 seconds @18:39:34.34 CS.seek 3ml @18:39:42.96 CSV.dial :waste @18:39:43.24 delay 3 seconds @18:39:43.50
CS.seek 0ml @18:39:46.26 ATV.dial :air3 @18:39:47.31 delay 3 seconds @18:39:50.39 AS.seek 0.794ml @18:39:52.29
CSV.dial :flush @18:39:52.81 CS.seek 3ml @18:39:55.71 delay 3 seconds @18:39:58.73 ATV.dial :bleach @18:39:59.37 AS.seek 0.779ml @18:40:01.46
delay 5 seconds @18:40:02.70 delay 3 seconds @18:40:05.72 ATV.dial :AIV @18:40:06.48
CSV.dial :air @18:40:07.00 AS.reconfigure ASconfig @18:40:07.06 AS.seek 0.015ml @18:40:07.19
CS.seek 5ml @18:40:13.40 CSV.dial :air @18:40:13.42 CS.seek 0ml @18:40:27.08 delay 7 seconds @18:40:34.12 CS.seek 3ml @18:40:42.77 CSV.dial :waste @18:40:43.63 CS.seek 0ml @18:40:51.29 ATV.dial :waste @18:40:52.33
Flushing lower manifold's waste1 port @18:40:52.47 CSV.dial :flush @18:40:52.80 AS.seek :empty @18:40:53.00
CS.seek 5ml @18:40:53.99 ATV.dial :air3 @18:40:54.68 AS.reconfigure ASslow1 @18:40:54.74 AS.seek 0.009ml @18:40:57.10 delay 2 seconds @18:40:59.12 ATV.dial :bleach @18:40:59.59 AS.seek 0.579ml @18:41:06.87
delay 5 seconds @18:41:11.89 CSV.dial :air @18:41:12.48 CS.seek 7ml @18:41:18.68 CSV.dial CTV @18:41:18.71
CTV.dial CSV @18:41:20.32 CBV.dial :waste1 @18:41:20.85 CS.seek 0ml @18:41:39.59 Flushing lower manifold's waste2b port @18:41:39.73 CSV.dial :flush @18:41:40.58 CS.seek 5ml @18:41:54.30 delay 5 seconds @18:41:59.32 CSV.dial :air @18:41:59.94 CS.seek 7ml @18:42:06.15 CSV.dial CTV @18:42:06.18
CTV.dial CSV @18:42:06.69 CBV.dial :waste2b @18:42:07.56 CS.seek 0ml @18:42:26.59 Garaging FlushPuck in Collection Clamp @18:42:26.68 Elbow.reconfigure TightElbow @18:42:26.74 Elbow.seek CC @18:42:26.88 Elbow.reconfigure LooseElbow @18:42:26.99 Forearm.seek CC @18:42:27.92 Hand.close @18:42:28.65 CC.seek :open @18:42:33.05 Forearm.seek :retract @18:42:34.03 Elbow.reconfigure TightElbow @18:42:34.10 Elbow.seek :garage @18:42:37.34 Elbow.reconfigure LooseElbow @18:42:37.45 Forearm.reconfigure TightForearm @18:42:37.51 Forearm.seek :garage @18:42:38.69 Hand.open @18:42:39.91 Forearm.reconfigure LooseForearm @18:42:39.98 Forearm.seek :retract @18:42:41.03 Starting initialPurge @18:42:41.04 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:42:41.14 Hand.open @18:42:41.45 Elbow.reconfigure TightElbow @18:42:41.51 Elbow.seek FlushPuck::Garage @18:42:41.66 Elbow.reconfigure LooseElbow @18:42:41.77 Forearm.seek :garage @18:42:42.82 Hand.close @18:42:43.51 Forearm.seek :retract @18:42:44.55 Elbow.reconfigure TightElbow @18:42:44.61 Elbow.seek CC @18:42:47.59 Elbow.reconfigure LooseElbow @18:42:47.70 Forearm.reconfigure TightForearm @18:42:47.76 Forearm.seek CC @18:42:49.03 CC.seek :closed @18:42:53.30 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.26 @18:42:53.38 Hand.open @18:42:54.08 Forearm.reconfigure LooseForearm @18:42:54.14 Forearm.seek :retract @18:42:54.56 delay 3 seconds @18:42:55.14
Flushing the Collection Valve::Manifold @18:42:55.27 CSV.dial CSR @18:42:55.85 Pulling 5ml of CSR.flush CSR.select :flush @18:42:55.99 CS.reconfigure CSconfig @18:42:56.06 CS.seek 5ml @18:42:57.58 ATV.dial :air3 @18:42:58.15 delay 3 seconds @18:43:01.22 AS.seek 0.604ml @18:43:06.56 delay 3 seconds @18:43:09.58 ATV.dial :bleach @18:43:09.84
delay 5 seconds @18:43:10.21 AS.seek 0.589ml @18:43:13.53 delay 3 seconds @18:43:14.86
CSR.select :air @18:43:15.03 CS.reconfigure CSfast @18:43:15.09 CS.seek 7.5ml @18:43:16.55 ATV.dial :ASV @18:43:17.59 ASV.dial :wasteR @18:43:18.21 AS.seek 0ml @18:43:22.46
CSV.dial :waste @18:43:23.98 CS.reconfigure CSconfig @18:43:24.05 CS.seek 0ml @18:43:44.24 Purging external Intake @18:43:44.37 CSV.dial :flush @18:43:44.88 CS.reconfigure CSfast @18:43:44.94 CS.seek 10ml @18:44:11.56 delay 5 seconds @18:44:16.59 CSV.dial CBV @18:44:16.62
CBV.dial CSV @18:44:17.54 CTV.dial :intake @18:44:18.40 Intake.select :open @18:44:18.53 CS.seek 0ml @18:44:46.09 Intake.select :close @18:44:46.20 CTV.dial :air @18:44:47.06 Flushing upper manifold @18:44:47.19 CSV.dial :flush @18:44:48.05 CS.seek 5ml @18:45:01.87 delay 5 seconds @18:45:06.89 CSV.dial :air @18:45:07.47 CS.seek 7ml @18:45:13.56 CSV.dial CBV @18:45:13.59
CBV.dial CSV @18:45:15.20 CTV.dial :air @18:45:15.29 CS.seek 0ml @18:45:16.99 delay 1:00 @18:45:34.03
delay 7 seconds @18:45:41.06 CS.seek 3ml @18:45:49.81 CSV.dial :waste @18:45:50.35 CS.seek 0ml @18:45:59.04 CSV.dial :flush @18:45:59.57 CS.seek 3ml @18:46:08.43 delay 5 seconds @18:46:13.45 CSV.dial :air @18:46:14.04 CS.seek 5ml @18:46:17.01 AFV.select :carrierSolution @18:46:17.13 delay 2 seconds @18:46:19.15 AS.reconfigure ASconfig @18:46:19.21 AS.seek 1ml @18:46:20.18
CSV.dial :air @18:46:20.20 CS.seek 0ml @18:46:33.87 delay 7 seconds @18:46:40.90 CS.seek 3ml @18:46:49.56 CSV.dial :waste @18:46:50.43 CS.seek 0ml @18:46:58.96 Garaging FlushPuck in Collection Clamp @18:46:59.05 Elbow.reconfigure TightElbow @18:46:59.11 Elbow.seek CC @18:46:59.35 Elbow.reconfigure LooseElbow @18:46:59.45 Forearm.seek CC @18:47:00.45 Hand.close @18:47:01.16 CC.seek :open @18:47:05.55 Forearm.seek :retract @18:47:06.52 Elbow.reconfigure TightElbow @18:47:06.59 Elbow.seek :garage @18:47:09.69 Elbow.reconfigure LooseElbow @18:47:09.79 Forearm.reconfigure TightForearm @18:47:09.85 Forearm.seek :garage @18:47:11.05 Hand.open @18:47:11.83 Forearm.reconfigure LooseForearm @18:47:11.89 Forearm.seek :retract @18:47:12.96 Completed [postDeployPhase] begun 17:25:52 @18:47:12.98 Extending 18tun_postDeploy until completion of MFBPCR @18:47:16.96 delay 1 second @18:47:17.98 AFV.select :holdingCoil @18:47:18.09 delay 1 second @18:47:19.10 ATV.dial ASV @18:47:19.11 ASV.dial :wasteR @18:47:19.15 AS.reconfigure ASslow1 @18:47:19.21 AS.seek 0ml @18:50:40.62 delay 20 seconds @18:51:00.65 ATV.dial :air3 @18:51:02.16 AS.seek 0.59ml @18:53:01.18 delay 3 seconds @18:53:04.20 ATV.dial :ASV @18:53:05.62 ASV.dial :wasteR @18:53:05.71 AS.seek 0.09ml @18:54:46.53 delay 30 seconds @18:55:16.55 ATV.dial :extSrc @18:55:17.32 delay 3 seconds @18:55:20.34 ATV.dial :ElutionWater @18:55:21.46 AS.seek 0.12ml @18:55:27.81 ATV.dial :air3 @18:55:28.43 AS.seek 0.13ml @18:55:30.73 delay 2 seconds @18:55:32.75 ATV.dial :bleach @18:55:33.22 AS.seek 0.23ml @18:55:53.65 delay 3 seconds @18:55:56.67 ATV.dial :air3 @18:55:57.23 delay 3 seconds @18:56:00.30 AS.seek 0.255ml @18:56:05.60 delay 3 seconds @18:56:08.62 ATV.dial :bleach @18:56:09.24 AS.seek 0.24ml @18:56:12.51 delay 3 seconds @18:56:15.53 ATV.dial :ASV @18:56:16.56 ASV.dial :SPEdelivery @18:56:16.95 AFV.select :carrierSolution @18:56:17.07 delay 2 seconds @18:56:19.09 AS.reconfigure ASconfig @18:56:19.14 AS.seek 0.5ml @18:56:34.40 delay 1 second @18:56:35.42 AFV.select :holdingCoil @18:56:35.58 AS.reconfigure ASslow1 @18:56:35.64 AS.seek 0.17ml @18:57:42.30 SPE.hold 2:46:40,95C @18:57:42.80 delay 3:00 @19:00:42.84 AFV.select :carrierSolution @19:00:42.96 delay 2 seconds @19:00:44.98 AS.reconfigure ASconfig @19:00:45.03 AS.seek 1ml @19:01:33.03 delay 1 second @19:01:34.05 AFV.select :holdingCoil @19:01:34.16 delay 1 second @19:01:35.17 ASV.dial :wasteD @19:01:36.27 AS.seek 0ml @19:02:34.07 Priming Eluate @19:02:34.08 AFV.select :carrierSolution @19:02:34.21 delay 2 seconds @19:02:36.23 AS.seek 0.5ml @19:03:05.26 delay 1 second @19:03:06.28 AFV.select :holdingCoil @19:03:06.38 ATV.dial ASV @19:03:06.40 ASV.dial :SPErecovery @19:03:07.03 Swish #1 of 2 @19:03:07.12 AS.reconfigure ASslow0 @19:03:07.18 AS.seek 0.6ml @19:03:17.63 AS.seek 0.5ml @19:03:28.01 Swish #2 of 2 @19:03:28.10 AS.seek 0.6ml @19:03:38.57 AS.seek 0.5ml @19:03:48.95 delay 3 seconds @19:03:52.02 AS.reconfigure ASslow1 @19:03:52.08 AS.seek 0.684ml @19:04:29.39 delay 3 seconds @19:04:32.41 Eluate primed @19:04:32.42 ATV.dial :waste2 @19:04:33.07 AS.reconfigure ASnorm @19:04:33.13 AS.seek 0ml @19:05:07.93 SPE.coast @19:05:08.01 ASV.dial :SPErecovery @19:05:08.03 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118nov1919h.pcr @19:05:08.06 Building PCR Slug from ASV with MM2 mix, PP4 primer @19:05:08.09 Priming with carrier @19:05:08.11 ATV.dial ARV @19:05:09.20 ARV.dial :carrier @19:05:09.58 AS.seek 0.5ml @19:05:35.10 AFV.select :carrierSolution @19:05:35.23 delay 2 seconds @19:05:37.25 AS.reconfigure ASconfig @19:05:37.32 AS.seek 1ml @19:06:06.37 delay 1 second @19:06:07.39 AFV.select :holdingCoil @19:06:07.50 delay 1 second @19:06:08.51 ATV.dial :waste @19:06:10.02 AS.reconfigure ASnorm @19:06:10.08 AS.seek :empty @19:07:00.89 AFV.select :carrierSolution @19:07:01.02 delay 2 seconds @19:07:03.04 AS.reconfigure ASconfig @19:07:03.11 AS.seek 0.5ml @19:07:32.20 delay 1 second @19:07:33.22 AFV.select :holdingCoil @19:07:33.33 Building Slug @19:07:33.34 ATV.dial :ARV @19:07:34.96 delay 2 seconds @19:07:36.98 ARV.dial :air1 @19:07:37.40 delay 2 seconds @19:07:39.47 AS.reconfigure ASslow2 @19:07:39.54 AS.seek 0.506ml @19:07:45.78 delay 2 seconds @19:07:47.80 ARV.dial :MM2 @19:07:49.34 delay 2 seconds @19:07:51.37 Adding MM2 @19:07:51.45 AS.seek 0.524ml @19:08:09.79 delay 25 seconds @19:08:34.81 ARV.dial :PP4 @19:08:35.21 Adding PP4 @19:08:35.23 delay 2 seconds @19:08:37.30 AS.seek 0.53ml @19:08:43.56 delay 10 seconds @19:08:53.59 ARV.dial :air3 @19:08:54.00 adding ARV air @19:08:54.02 delay 2 seconds @19:08:56.09 AS.seek 0.536ml @19:09:02.32 delay 5 seconds @19:09:07.34 ARV.dial :carrier @19:09:08.67 delay 7 seconds @19:09:15.76 AS.seek 0.594ml @19:10:14.29 delay 5 seconds @19:10:19.31 ATV.dial :ASV @19:10:19.70 delay 2 seconds @19:10:21.78 AS.seek 0.6ml @19:10:28.12 delay 4 seconds @19:10:32.14 ATV.dial ARV @19:10:32.68 delay 2 seconds @19:10:34.70 ARV.dial :carrier @19:10:34.72 delay 2 seconds @19:10:36.79 AS.seek 0.65ml @19:11:27.42 delay 4 seconds @19:11:31.44 ATV.dial PCR @19:11:32.46 ASV.dial :waste1 @19:11:33.34 ARV.dial :air1 @19:11:33.75 Positioning Slug by 0.324ml @19:11:33.85 AS.seek 0.326ml @19:16:59.90 delay 5 seconds @19:17:04.92 ATV.dial ARV @19:17:05.70 ASV.dial :PCRlock @19:17:06.18 delay 5 seconds @19:17:11.21 Running Tt1 Profile60C300 thermocycling @19:17:11.22 PCR.program Profile60C300 @19:17:11.37 Profile60C300 should complete in < 1:45:21 @19:17:11.38 PCR.run 2:16:41.25 @20:39:17.02 PCR.off @20:39:18.75 PCR for Tt1 completed -- closed /var/log/esp/Tt118nov1919h.pcr @20:39:18.83 Cleaning PCR @20:39:18.84 AFV.select :carrierSolution @20:39:18.97 delay 2 seconds @20:39:20.99 AS.reconfigure ASconfig @20:39:21.05 AS.seek 0.5ml @20:39:31.37 delay 1 second @20:39:32.39 AFV.select :holdingCoil @20:39:32.49 ASV.dial :waste1 @20:39:32.89 ATV.dial :air3 @20:39:34.17 AS.reconfigure ASslow1 @20:39:34.23 AS.seek 0.51ml @20:39:36.54 delay 2 seconds @20:39:38.56 ATV.dial :bleach @20:39:39.18 AS.seek 0.81ml @20:40:39.80 delay 3 seconds @20:40:42.82 ATV.dial :air3 @20:40:43.39 delay 3 seconds @20:40:46.47 AS.seek 0.835ml @20:40:51.79 delay 3 seconds @20:40:54.81 ATV.dial :bleach @20:40:55.43 AS.seek 0.82ml @20:40:58.76 delay 3 seconds @20:41:01.78 ARV.dial :waste @20:41:02.42 delay 3 seconds @20:41:05.44 ATV.dial :ARV @20:41:06.32 AS.reconfigure ASconfig @20:41:06.38 AS.seek 0.47ml @20:41:26.80 AFV.select :carrierSolution @20:41:26.92 delay 2 seconds @20:41:28.94 AS.seek 1ml @20:41:59.72 delay 1 second @20:42:00.74 AFV.select :holdingCoil @20:42:00.85 delay 1 second @20:42:01.87 AS.reconfigure ASfast @20:42:01.93 AS.seek 0ml @20:42:29.30 AFV.select :carrierSolution @20:42:29.42 delay 2 seconds @20:42:31.44 AS.reconfigure ASconfig @20:42:31.50 AS.seek 1ml @20:43:29.26 delay 1 second @20:43:30.28 AFV.select :holdingCoil @20:43:30.39 delay 1 second @20:43:31.41 AS.reconfigure ASfast @20:43:31.47 AS.seek 0ml @20:43:58.83 AFV.select :carrierSolution @20:43:58.95 delay 2 seconds @20:44:00.97 AS.reconfigure ASconfig @20:44:01.03 AS.seek 1ml @20:44:58.80 delay 1 second @20:44:59.82 AFV.select :holdingCoil @20:44:59.93 delay 1 second @20:45:00.95 AS.reconfigure ASfast @20:45:01.01 AS.seek 0ml @20:45:28.36 ARV.dial between carrier and air1 @20:45:28.87 ATV.dial :air3 @20:45:30.15 AS.reconfigure ASslow1 @20:45:30.21 AS.seek 0.01ml @20:45:32.55 delay 2 seconds @20:45:34.57 ATV.dial :bleach @20:45:35.20 AS.seek 0.81ml @20:48:16.34 delay 3 seconds @20:48:19.36 ATV.dial :air3 @20:48:19.92 delay 3 seconds @20:48:23.00 AS.seek 0.835ml @20:48:28.31 delay 3 seconds @20:48:31.33 ATV.dial :bleach @20:48:31.95 AS.seek 0.82ml @20:48:35.26 delay 3 seconds @20:48:38.28 ATV.dial PCR @20:48:38.69 AS.reconfigure ASconfig @20:48:38.75 AS.seek :empty @20:49:26.22 PCR.program :bleachProfile @20:49:26.28 PCR program should complete in < 2:45 @20:49:26.29 PCR.run 10:00 @20:52:25.09 PCR.off @20:52:25.13 AFV.select :carrierSolution @20:52:25.25 delay 2 seconds @20:52:27.27 AS.seek 1ml @20:53:25.55 delay 1 second @20:53:26.62 AFV.select :holdingCoil @20:53:26.73 delay 1 second @20:53:27.75 AS.reconfigure ASfast @20:53:27.82 AS.seek 0ml @20:53:55.19 delay 3 seconds @20:53:58.21 AFV.select :carrierSolution @20:53:58.32 delay 2 seconds @20:54:00.34 AS.reconfigure ASconfig @20:54:00.40 AS.seek 1ml @20:54:58.19 delay 1 second @20:54:59.21 AFV.select :holdingCoil @20:54:59.32 delay 1 second @20:55:00.34 AS.reconfigure ASfast @20:55:00.41 AS.seek 0ml @20:55:27.76 delay 3 seconds @20:55:30.78 AFV.select :carrierSolution @20:55:30.89 delay 2 seconds @20:55:32.91 AS.reconfigure ASconfig @20:55:32.99 AS.seek 1ml @20:56:30.73 delay 1 second @20:56:31.75 AFV.select :holdingCoil @20:56:31.86 delay 1 second @20:56:32.88 AS.reconfigure ASfast @20:56:32.95 AS.seek 0ml @20:57:00.33 delay 3 seconds @20:57:03.35 AFV.select :carrierSolution @20:57:03.46 delay 2 seconds @20:57:05.48 AS.reconfigure ASconfig @20:57:05.54 AS.seek 1ml @20:58:03.33 delay 1 second @20:58:04.35 AFV.select :holdingCoil @20:58:04.46 delay 1 second @20:58:05.48 AS.reconfigure ASfast @20:58:05.54 AS.seek 0ml @20:58:32.89 delay 3 seconds @20:58:35.91 AFV.select :carrierSolution @20:58:36.02 delay 2 seconds @20:58:38.04 AS.reconfigure ASconfig @20:58:38.11 AS.seek 1ml @20:59:35.95 delay 1 second @20:59:36.97 AFV.select :holdingCoil @20:59:37.08 delay 1 second @20:59:38.10 AS.reconfigure ASnorm @20:59:38.16 AS.seek 0ml @21:00:28.91 Setting up PCR for Rp -- writing /var/log/esp/Rp18nov1921h.pcr @21:00:28.93 Building PCR Slug from ASV with MM2 mix, PP3 primer @21:00:28.96 Priming with carrier @21:00:28.98 ATV.dial ARV @21:00:29.59 ARV.dial :carrier @21:00:29.93 AS.seek 0.5ml @21:00:55.53 AFV.select :carrierSolution @21:00:55.66 delay 2 seconds @21:00:57.68 AS.reconfigure ASconfig @21:00:57.75 AS.seek 1ml @21:01:26.77 delay 1 second @21:01:27.79 AFV.select :holdingCoil @21:01:27.90 delay 1 second @21:01:28.91 ATV.dial :waste @21:01:30.57 AS.reconfigure ASnorm @21:01:30.63 AS.seek :empty @21:02:21.41 AFV.select :carrierSolution @21:02:21.54 delay 2 seconds @21:02:23.56 AS.reconfigure ASconfig @21:02:23.63 AS.seek 0.5ml @21:02:52.72 delay 1 second @21:02:53.74 AFV.select :holdingCoil @21:02:53.85 Building Slug @21:02:53.86 ATV.dial :ARV @21:02:55.50 delay 2 seconds @21:02:57.52 ARV.dial :air1 @21:02:57.93 delay 2 seconds @21:03:00.00 AS.reconfigure ASslow2 @21:03:00.07 AS.seek 0.506ml @21:03:06.34 delay 2 seconds @21:03:08.36 ARV.dial :MM2 @21:03:09.95 delay 2 seconds @21:03:11.97 Adding MM2 @21:03:12.05 AS.seek 0.524ml @21:03:30.50 delay 25 seconds @21:03:55.52 ARV.dial :PP3 @21:03:55.92 Adding PP3 @21:03:55.94 delay 2 seconds @21:03:58.01 AS.seek 0.53ml @21:04:04.33 delay 10 seconds @21:04:14.36 ARV.dial :air2 @21:04:14.77 adding ARV air @21:04:14.79 delay 2 seconds @21:04:16.87 AS.seek 0.536ml @21:04:23.23 delay 5 seconds @21:04:28.25 ARV.dial :carrier @21:04:29.56 delay 7 seconds @21:04:36.64 AS.seek 0.594ml @21:05:35.36 delay 5 seconds @21:05:40.38 ATV.dial :ASV @21:05:40.78 delay 2 seconds @21:05:42.85 AS.seek 0.6ml @21:05:49.17 delay 4 seconds @21:05:53.19 ATV.dial ARV @21:05:53.73 delay 2 seconds @21:05:55.75 ARV.dial :carrier @21:05:55.77 delay 2 seconds @21:05:57.84 AS.seek 0.65ml @21:06:48.42 delay 4 seconds @21:06:52.44 ATV.dial PCR @21:06:53.05 ASV.dial :waste1 @21:06:53.06 ARV.dial :air1 @21:06:53.98 Positioning Slug by 0.324ml @21:06:54.08 AS.seek 0.326ml @21:12:19.94 delay 5 seconds @21:12:24.96 ATV.dial ARV @21:12:25.73 ASV.dial :PCRlock @21:12:26.13 delay 5 seconds @21:12:31.16 Running Rp Profile60C300 thermocycling @21:12:31.17 PCR.program Profile60C300 @21:12:31.32 Profile60C300 should complete in < 1:45:21 @21:12:31.33 PCR.run 2:16:41.25 @22:34:33.48 PCR.off @22:34:35.50 PCR for Rp completed -- closed /var/log/esp/Rp18nov1921h.pcr @22:34:35.58 Cleaning PCR @22:34:35.59 AFV.select :carrierSolution @22:34:35.71 delay 2 seconds @22:34:37.73 AS.reconfigure ASconfig @22:34:37.79 AS.seek 0.5ml @22:34:48.10 delay 1 second @22:34:49.12 AFV.select :holdingCoil @22:34:49.22 ASV.dial :waste1 @22:34:49.61 ATV.dial :air3 @22:34:50.89 AS.reconfigure ASslow1 @22:34:50.95 AS.seek 0.51ml @22:34:53.26 delay 2 seconds @22:34:55.28 ATV.dial :bleach @22:34:55.91 AS.seek 0.81ml @22:35:56.54 delay 3 seconds @22:35:59.56 ATV.dial :air3 @22:36:00.13 delay 3 seconds @22:36:03.21 AS.seek 0.835ml @22:36:08.50 delay 3 seconds @22:36:11.52 ATV.dial :bleach @22:36:12.14 AS.seek 0.82ml @22:36:15.46 delay 3 seconds @22:36:18.48 ARV.dial :waste @22:36:19.10 delay 3 seconds @22:36:22.12 ATV.dial :ARV @22:36:23.00 AS.reconfigure ASconfig @22:36:23.06 AS.seek 0.47ml @22:36:43.46 AFV.select :carrierSolution @22:36:43.58 delay 2 seconds @22:36:45.60 AS.seek 1ml @22:37:16.39 delay 1 second @22:37:17.41 AFV.select :holdingCoil @22:37:17.52 delay 1 second @22:37:18.54 AS.reconfigure ASfast @22:37:18.60 AS.seek 0ml @22:37:45.94 AFV.select :carrierSolution @22:37:46.06 delay 2 seconds @22:37:48.08 AS.reconfigure ASconfig @22:37:48.14 AS.seek 1ml @22:38:45.91 delay 1 second @22:38:46.93 AFV.select :holdingCoil @22:38:47.04 delay 1 second @22:38:48.06 AS.reconfigure ASfast @22:38:48.12 AS.seek 0ml @22:39:15.47 AFV.select :carrierSolution @22:39:15.59 delay 2 seconds @22:39:17.61 AS.reconfigure ASconfig @22:39:17.67 AS.seek 1ml @22:40:15.43 delay 1 second @22:40:16.45 AFV.select :holdingCoil @22:40:16.56 delay 1 second @22:40:17.58 AS.reconfigure ASfast @22:40:17.64 AS.seek 0ml @22:40:44.99 ARV.dial between carrier and air1 @22:40:45.50 ATV.dial :air3 @22:40:46.78 AS.reconfigure ASslow1 @22:40:46.84 AS.seek 0.01ml @22:40:49.16 delay 2 seconds @22:40:51.18 ATV.dial :bleach @22:40:51.80 AS.seek 0.81ml @22:43:32.94 delay 3 seconds @22:43:35.96 ATV.dial :air3 @22:43:36.52 delay 3 seconds @22:43:39.59 AS.seek 0.835ml @22:43:44.89 delay 3 seconds @22:43:47.91 ATV.dial :bleach @22:43:48.53 AS.seek 0.82ml @22:43:51.83 delay 3 seconds @22:43:54.85 ATV.dial PCR @22:43:55.25 AS.reconfigure ASconfig @22:43:55.31 AS.seek :empty @22:44:42.79 PCR.program :bleachProfile @22:44:42.85 PCR program should complete in < 2:45 @22:44:42.86 PCR.run 10:00 @22:47:41.67 PCR.off @22:47:41.71 AFV.select :carrierSolution @22:47:41.83 delay 2 seconds @22:47:43.85 AS.seek 1ml @22:48:41.71 delay 1 second @22:48:42.73 AFV.select :holdingCoil @22:48:42.84 delay 1 second @22:48:43.86 AS.reconfigure ASfast @22:48:43.92 AS.seek 0ml @22:49:11.27 delay 3 seconds @22:49:14.29 AFV.select :carrierSolution @22:49:14.40 delay 2 seconds @22:49:16.42 AS.reconfigure ASconfig @22:49:16.48 AS.seek 1ml @22:50:14.75 delay 1 second @22:50:15.77 AFV.select :holdingCoil @22:50:15.88 delay 1 second @22:50:16.90 AS.reconfigure ASfast @22:50:16.97 AS.seek 0ml @22:50:44.33 delay 3 seconds @22:50:47.35 AFV.select :carrierSolution @22:50:47.46 delay 2 seconds @22:50:49.48 AS.reconfigure ASconfig @22:50:49.54 AS.seek 1ml @22:51:47.29 delay 1 second @22:51:48.31 AFV.select :holdingCoil @22:51:48.42 delay 1 second @22:51:49.44 AS.reconfigure ASfast @22:51:49.51 AS.seek 0ml @22:52:16.85 delay 3 seconds @22:52:19.87 AFV.select :carrierSolution @22:52:19.98 delay 2 seconds @22:52:22.00 AS.reconfigure ASconfig @22:52:22.07 AS.seek 1ml @22:53:19.86 delay 1 second @22:53:20.88 AFV.select :holdingCoil @22:53:20.99 delay 1 second @22:53:22.01 AS.reconfigure ASfast @22:53:22.08 AS.seek 0ml @22:53:49.44 delay 3 seconds @22:53:52.46 AFV.select :carrierSolution @22:53:52.57 delay 2 seconds @22:53:54.60 AS.reconfigure ASconfig @22:53:54.66 AS.seek 1ml @22:54:52.46 delay 1 second @22:54:53.48 AFV.select :holdingCoil @22:54:53.59 delay 1 second @22:54:54.61 AS.reconfigure ASnorm @22:54:54.67 AS.seek 0ml @22:55:45.40 Setting up PCR for Bb -- writing /var/log/esp/Bb18nov1922h.pcr @22:55:45.42 Building PCR Slug from ASV with MM3 mix, PP5 primer @22:55:45.43 Priming with carrier @22:55:45.46 ATV.dial ARV @22:55:46.08 ARV.dial :carrier @22:55:46.42 AS.seek 0.5ml @22:56:12.00 AFV.select :carrierSolution @22:56:12.13 delay 2 seconds @22:56:14.15 AS.reconfigure ASconfig @22:56:14.22 AS.seek 1ml @22:56:43.25 delay 1 second @22:56:44.27 AFV.select :holdingCoil @22:56:44.38 delay 1 second @22:56:45.39 ATV.dial :waste @22:56:47.04 AS.reconfigure ASnorm @22:56:47.11 AS.seek :empty @22:57:37.88 AFV.select :carrierSolution @22:57:38.00 delay 2 seconds @22:57:40.02 AS.reconfigure ASconfig @22:57:40.09 AS.seek 0.5ml @22:58:09.18 delay 1 second @22:58:10.20 AFV.select :holdingCoil @22:58:10.31 Building Slug @22:58:10.32 ATV.dial :ARV @22:58:11.96 delay 2 seconds @22:58:13.98 ARV.dial :air1 @22:58:14.38 delay 2 seconds @22:58:16.45 AS.reconfigure ASslow2 @22:58:16.52 AS.seek 0.506ml @22:58:22.69 delay 2 seconds @22:58:24.71 ARV.dial :MM3 @22:58:25.79 delay 2 seconds @22:58:27.82 Adding MM3 @22:58:27.91 AS.seek 0.524ml @22:58:46.27 delay 25 seconds @22:59:11.29 ARV.dial :PP5 @22:59:11.69 Adding PP5 @22:59:11.71 delay 2 seconds @22:59:13.79 AS.seek 0.53ml @22:59:20.22 delay 10 seconds @22:59:30.25 ARV.dial :air3 @22:59:30.65 adding ARV air @22:59:30.67 delay 2 seconds @22:59:32.74 AS.seek 0.536ml @22:59:38.91 delay 5 seconds @22:59:43.93 ARV.dial :carrier @22:59:45.22 delay 7 seconds @22:59:52.31 AS.seek 0.594ml @23:00:50.88 delay 5 seconds @23:00:55.90 ATV.dial :ASV @23:00:56.30 delay 2 seconds @23:00:58.38 AS.seek 0.6ml @23:01:04.65 delay 4 seconds @23:01:08.67 ATV.dial ARV @23:01:09.21 delay 2 seconds @23:01:11.23 ARV.dial :carrier @23:01:11.25 delay 2 seconds @23:01:13.33 AS.seek 0.65ml @23:02:04.11 delay 4 seconds @23:02:08.13 ATV.dial PCR @23:02:08.74 ASV.dial :waste1 @23:02:08.75 ARV.dial :air1 @23:02:09.20 Positioning Slug by 0.324ml @23:02:09.29 AS.seek 0.326ml @23:07:35.27 delay 5 seconds @23:07:40.29 ATV.dial ARV @23:07:41.53 ASV.dial :PCRlock @23:07:41.94 delay 5 seconds @23:07:46.97 Running Bb Profile60C300 thermocycling @23:07:46.98 PCR.program Profile60C300 @23:07:47.12 Profile60C300 should complete in < 1:45:21 @23:07:47.14 PCR.run 2:16:41.25 @00:29:55.80CET20-Nov-18 PCR.off @00:29:57.32 PCR for Bb completed -- closed /var/log/esp/Bb18nov1922h.pcr @00:29:57.40 Cleaning PCR @00:29:57.41 AFV.select :carrierSolution @00:29:57.54 delay 2 seconds @00:29:59.56 AS.reconfigure ASconfig @00:29:59.62 AS.seek 0.5ml @00:30:09.93 delay 1 second @00:30:10.95 AFV.select :holdingCoil @00:30:11.05 ASV.dial :waste1 @00:30:11.43 ATV.dial :air3 @00:30:12.69 AS.reconfigure ASslow1 @00:30:13.26 AS.seek 0.51ml @00:30:15.54 delay 2 seconds @00:30:17.56 ATV.dial :bleach @00:30:18.18 AS.seek 0.81ml @00:31:18.79 delay 3 seconds @00:31:21.81 ATV.dial :air3 @00:31:22.37 delay 3 seconds @00:31:25.44 AS.seek 0.835ml @00:31:30.83 delay 3 seconds @00:31:33.85 ATV.dial :bleach @00:31:34.48 AS.seek 0.82ml @00:31:37.76 delay 3 seconds @00:31:40.78 ARV.dial :waste @00:31:41.40 delay 3 seconds @00:31:44.42 ATV.dial :ARV @00:31:45.30 AS.reconfigure ASconfig @00:31:45.36 AS.seek 0.47ml @00:32:05.78 AFV.select :carrierSolution @00:32:05.90 delay 2 seconds @00:32:07.92 AS.seek 1ml @00:32:38.71 delay 1 second @00:32:39.73 AFV.select :holdingCoil @00:32:39.84 delay 1 second @00:32:40.86 AS.reconfigure ASfast @00:32:40.93 AS.seek 0ml @00:33:08.28 AFV.select :carrierSolution @00:33:08.40 delay 2 seconds @00:33:10.42 AS.reconfigure ASconfig @00:33:10.48 AS.seek 1ml @00:34:08.28 delay 1 second @00:34:09.30 AFV.select :holdingCoil @00:34:09.41 delay 1 second @00:34:10.43 AS.reconfigure ASfast @00:34:10.49 AS.seek 0ml @00:34:37.84 AFV.select :carrierSolution @00:34:37.96 delay 2 seconds @00:34:39.98 AS.reconfigure ASconfig @00:34:40.04 AS.seek 1ml @00:35:37.84 delay 1 second @00:35:38.86 AFV.select :holdingCoil @00:35:38.97 delay 1 second @00:35:39.99 AS.reconfigure ASfast @00:35:40.05 AS.seek 0ml @00:36:07.40 ARV.dial between carrier and air1 @00:36:07.92 ATV.dial :air3 @00:36:09.21 AS.reconfigure ASslow1 @00:36:09.27 AS.seek 0.01ml @00:36:11.63 delay 2 seconds @00:36:13.65 ATV.dial :bleach @00:36:14.26 AS.seek 0.81ml @00:38:55.40 delay 3 seconds @00:38:58.42 ATV.dial :air3 @00:38:58.98 delay 3 seconds @00:39:02.05 AS.seek 0.835ml @00:39:07.36 delay 3 seconds @00:39:10.38 ATV.dial :bleach @00:39:11.01 AS.seek 0.82ml @00:39:14.31 delay 3 seconds @00:39:17.33 ATV.dial PCR @00:39:17.73 AS.reconfigure ASconfig @00:39:17.79 AS.seek :empty @00:40:05.26 PCR.program :bleachProfile @00:40:05.32 PCR program should complete in < 2:45 @00:40:05.33 PCR.run 10:00 @00:43:04.13 PCR.off @00:43:04.17 AFV.select :carrierSolution @00:43:04.29 delay 2 seconds @00:43:06.31 AS.seek 1ml @00:44:04.12 delay 1 second @00:44:05.14 AFV.select :holdingCoil @00:44:05.25 delay 1 second @00:44:06.27 AS.reconfigure ASfast @00:44:06.33 AS.seek 0ml @00:44:33.68 delay 3 seconds @00:44:36.70 AFV.select :carrierSolution @00:44:36.81 delay 2 seconds @00:44:38.83 AS.reconfigure ASconfig @00:44:38.90 AS.seek 1ml @00:45:36.68 delay 1 second @00:45:37.70 AFV.select :holdingCoil @00:45:37.81 delay 1 second @00:45:38.83 AS.reconfigure ASfast @00:45:38.89 AS.seek 0ml @00:46:06.24 delay 3 seconds @00:46:09.26 AFV.select :carrierSolution @00:46:09.37 delay 2 seconds @00:46:11.39 AS.reconfigure ASconfig @00:46:11.45 AS.seek 1ml @00:47:09.24 delay 1 second @00:47:10.26 AFV.select :holdingCoil @00:47:10.37 delay 1 second @00:47:11.39 AS.reconfigure ASfast @00:47:11.45 AS.seek 0ml @00:47:39.31 delay 3 seconds @00:47:42.33 AFV.select :carrierSolution @00:47:42.44 delay 2 seconds @00:47:44.46 AS.reconfigure ASconfig @00:47:44.52 AS.seek 1ml @00:48:42.31 delay 1 second @00:48:43.33 AFV.select :holdingCoil @00:48:43.44 delay 1 second @00:48:44.46 AS.reconfigure ASfast @00:48:44.53 AS.seek 0ml @00:49:11.89 delay 3 seconds @00:49:14.91 AFV.select :carrierSolution @00:49:15.02 delay 2 seconds @00:49:17.04 AS.reconfigure ASconfig @00:49:17.11 AS.seek 1ml @00:50:14.90 delay 1 second @00:50:15.92 AFV.select :holdingCoil @00:50:16.03 delay 1 second @00:50:17.05 AS.reconfigure ASnorm @00:50:17.11 AS.seek 0ml @00:51:07.83 Setting up PCR for Ss -- writing /var/log/esp/Ss18nov2000h.pcr @00:51:07.85 Building PCR Slug from ASV with MM1 mix, PP2 primer @00:51:07.86 Priming with carrier @00:51:07.89 ATV.dial ARV @00:51:08.51 ARV.dial :carrier @00:51:08.87 AS.seek 0.5ml @00:51:34.40 AFV.select :carrierSolution @00:51:34.53 delay 2 seconds @00:51:36.55 AS.reconfigure ASconfig @00:51:36.62 AS.seek 1ml @00:52:05.65 delay 1 second @00:52:06.67 AFV.select :holdingCoil @00:52:06.78 delay 1 second @00:52:07.79 ATV.dial :waste @00:52:09.44 AS.reconfigure ASnorm @00:52:09.50 AS.seek :empty @00:53:00.26 AFV.select :carrierSolution @00:53:00.39 delay 2 seconds @00:53:02.41 AS.reconfigure ASconfig @00:53:02.48 AS.seek 0.5ml @00:53:31.60 delay 1 second @00:53:32.62 AFV.select :holdingCoil @00:53:32.73 Building Slug @00:53:32.74 ATV.dial :ARV @00:53:34.37 delay 2 seconds @00:53:36.39 ARV.dial :air1 @00:53:36.80 delay 2 seconds @00:53:38.87 AS.reconfigure ASslow2 @00:53:38.94 AS.seek 0.506ml @00:53:45.14 delay 2 seconds @00:53:47.16 ARV.dial :MM1 @00:53:47.76 delay 2 seconds @00:53:49.79 Adding MM1 @00:53:49.86 AS.seek 0.524ml @00:54:08.18 delay 25 seconds @00:54:33.20 ARV.dial :PP2 @00:54:33.60 Adding PP2 @00:54:33.61 delay 2 seconds @00:54:35.70 AS.seek 0.53ml @00:54:41.92 delay 10 seconds @00:54:51.95 ARV.dial :air2 @00:54:52.35 adding ARV air @00:54:52.37 delay 2 seconds @00:54:54.44 AS.seek 0.536ml @00:55:00.75 delay 5 seconds @00:55:05.77 ARV.dial :carrier @00:55:07.07 delay 7 seconds @00:55:14.14 AS.seek 0.594ml @00:56:12.81 delay 5 seconds @00:56:17.83 ATV.dial :ASV @00:56:18.23 delay 2 seconds @00:56:20.31 AS.seek 0.6ml @00:56:26.64 delay 4 seconds @00:56:30.66 ATV.dial ARV @00:56:31.21 delay 2 seconds @00:56:33.23 ARV.dial :carrier @00:56:33.25 delay 2 seconds @00:56:35.32 AS.seek 0.65ml @00:57:25.98 delay 4 seconds @00:57:30.00 ATV.dial PCR @00:57:30.60 ASV.dial :waste1 @00:57:30.61 ARV.dial :air1 @00:57:31.04 Positioning Slug by 0.324ml @00:57:31.13 AS.seek 0.326ml @01:02:57.21 delay 5 seconds @01:03:02.23 ATV.dial ARV @01:03:03.00 ASV.dial :PCRlock @01:03:03.39 delay 5 seconds @01:03:08.42 Running Ss Profile60C350 thermocycling @01:03:08.43 PCR.program Profile60C350 @01:03:08.58 Profile60C350 should complete in < 1:48:05 @01:03:08.60 PCR.run 2:20:06.25 @02:25:41.87 PCR.off @02:25:43.85 PCR for Ss completed -- closed /var/log/esp/Ss18nov2000h.pcr @02:25:43.92 Cleaning PCR @02:25:43.93 AFV.select :carrierSolution @02:25:44.06 delay 2 seconds @02:25:46.08 AS.reconfigure ASconfig @02:25:46.14 AS.seek 0.5ml @02:25:56.47 delay 1 second @02:25:57.49 AFV.select :holdingCoil @02:25:57.59 ASV.dial :waste1 @02:25:57.97 ATV.dial :air3 @02:25:59.75 AS.reconfigure ASslow1 @02:25:59.81 AS.seek 0.51ml @02:26:02.12 delay 2 seconds @02:26:04.14 ATV.dial :bleach @02:26:04.76 AS.seek 0.81ml @02:27:05.37 delay 3 seconds @02:27:08.39 ATV.dial :air3 @02:27:08.95 delay 3 seconds @02:27:12.02 AS.seek 0.835ml @02:27:17.34 delay 3 seconds @02:27:20.36 ATV.dial :bleach @02:27:20.98 AS.seek 0.82ml @02:27:24.30 delay 3 seconds @02:27:27.32 ARV.dial :waste @02:27:27.95 delay 3 seconds @02:27:30.97 ATV.dial :ARV @02:27:31.86 AS.reconfigure ASconfig @02:27:31.92 AS.seek 0.47ml @02:27:52.34 AFV.select :carrierSolution @02:27:52.46 delay 2 seconds @02:27:54.48 AS.seek 1ml @02:28:25.22 delay 1 second @02:28:26.24 AFV.select :holdingCoil @02:28:26.35 delay 1 second @02:28:27.37 AS.reconfigure ASfast @02:28:27.44 AS.seek 0ml @02:28:54.80 AFV.select :carrierSolution @02:28:54.92 delay 2 seconds @02:28:56.94 AS.reconfigure ASconfig @02:28:57.00 AS.seek 1ml @02:29:54.80 delay 1 second @02:29:55.82 AFV.select :holdingCoil @02:29:55.93 delay 1 second @02:29:56.95 AS.reconfigure ASfast @02:29:57.02 AS.seek 0ml @02:30:24.37 AFV.select :carrierSolution @02:30:24.49 delay 2 seconds @02:30:26.51 AS.reconfigure ASconfig @02:30:26.57 AS.seek 1ml @02:31:24.31 delay 1 second @02:31:25.33 AFV.select :holdingCoil @02:31:25.44 delay 1 second @02:31:26.46 AS.reconfigure ASfast @02:31:26.53 AS.seek 0ml @02:31:53.91 ARV.dial between carrier and air1 @02:31:54.42 ATV.dial :air3 @02:31:55.70 AS.reconfigure ASslow1 @02:31:55.76 AS.seek 0.01ml @02:31:58.09 delay 2 seconds @02:32:00.11 ATV.dial :bleach @02:32:00.73 AS.seek 0.81ml @02:34:41.88 delay 3 seconds @02:34:44.90 ATV.dial :air3 @02:34:45.45 delay 3 seconds @02:34:48.52 AS.seek 0.835ml @02:34:53.84 delay 3 seconds @02:34:56.86 ATV.dial :bleach @02:34:57.48 AS.seek 0.82ml @02:35:00.80 delay 3 seconds @02:35:03.82 ATV.dial PCR @02:35:04.23 AS.reconfigure ASconfig @02:35:04.29 AS.seek :empty @02:35:51.78 PCR.program :bleachProfile @02:35:51.83 PCR program should complete in < 2:45 @02:35:51.84 PCR.run 10:00 @02:38:50.65 PCR.off @02:38:50.68 AFV.select :carrierSolution @02:38:50.81 delay 2 seconds @02:38:52.83 AS.seek 1ml @02:39:50.64 delay 1 second @02:39:51.66 AFV.select :holdingCoil @02:39:51.77 delay 1 second @02:39:52.79 AS.reconfigure ASfast @02:39:52.85 AS.seek 0ml @02:40:20.20 delay 3 seconds @02:40:23.22 AFV.select :carrierSolution @02:40:23.33 delay 2 seconds @02:40:25.35 AS.reconfigure ASconfig @02:40:25.42 AS.seek 1ml @02:41:23.22 delay 1 second @02:41:24.24 AFV.select :holdingCoil @02:41:24.35 delay 1 second @02:41:25.37 AS.reconfigure ASfast @02:41:25.43 AS.seek 0ml @02:41:52.77 delay 3 seconds @02:41:55.79 AFV.select :carrierSolution @02:41:55.90 delay 2 seconds @02:41:57.92 AS.reconfigure ASconfig @02:41:57.99 AS.seek 1ml @02:42:55.78 delay 1 second @02:42:56.80 AFV.select :holdingCoil @02:42:56.91 delay 1 second @02:42:58.44 AS.reconfigure ASfast @02:42:58.51 AS.seek 0ml @02:43:25.84 delay 3 seconds @02:43:28.86 AFV.select :carrierSolution @02:43:28.97 delay 2 seconds @02:43:30.99 AS.reconfigure ASconfig @02:43:31.05 AS.seek 1ml @02:44:28.86 delay 1 second @02:44:29.88 AFV.select :holdingCoil @02:44:29.99 delay 1 second @02:44:31.01 AS.reconfigure ASfast @02:44:31.08 AS.seek 0ml @02:44:58.44 delay 3 seconds @02:45:01.46 AFV.select :carrierSolution @02:45:01.57 delay 2 seconds @02:45:03.59 AS.reconfigure ASconfig @02:45:03.66 AS.seek 1ml @02:46:01.45 delay 1 second @02:46:02.47 AFV.select :holdingCoil @02:46:02.58 delay 1 second @02:46:03.60 AS.reconfigure ASnorm @02:46:03.66 AS.seek 0ml @02:46:54.40 Setting up PCR for IPc -- writing /var/log/esp/IPc18nov2002h.pcr @02:46:54.42 Building PCR Slug from ASV with MM1 mix, PP1 primer @02:46:54.44 Priming with carrier @02:46:54.45 ATV.dial ARV @02:46:55.08 ARV.dial :carrier @02:46:55.44 AS.seek 0.5ml @02:47:21.05 AFV.select :carrierSolution @02:47:21.18 delay 2 seconds @02:47:23.20 AS.reconfigure ASconfig @02:47:23.27 AS.seek 1ml @02:47:52.30 delay 1 second @02:47:53.32 AFV.select :holdingCoil @02:47:53.43 delay 1 second @02:47:54.44 ATV.dial :waste @02:47:56.10 AS.reconfigure ASnorm @02:47:56.16 AS.seek :empty @02:48:46.92 AFV.select :carrierSolution @02:48:47.05 delay 2 seconds @02:48:49.07 AS.reconfigure ASconfig @02:48:49.14 AS.seek 0.5ml @02:49:18.24 delay 1 second @02:49:19.26 AFV.select :holdingCoil @02:49:19.38 Building Slug @02:49:19.39 ATV.dial :ARV @02:49:21.02 delay 2 seconds @02:49:23.04 ARV.dial :air1 @02:49:23.44 delay 2 seconds @02:49:25.51 AS.reconfigure ASslow2 @02:49:25.58 AS.seek 0.506ml @02:49:31.75 delay 2 seconds @02:49:33.77 ARV.dial :MM1 @02:49:34.38 delay 2 seconds @02:49:36.41 Adding MM1 @02:49:36.49 AS.seek 0.524ml @02:49:54.80 delay 25 seconds @02:50:19.82 ARV.dial :PP1 @02:50:20.22 Adding PP1 @02:50:20.23 delay 2 seconds @02:50:22.32 AS.seek 0.53ml @02:50:28.59 delay 10 seconds @02:50:38.62 ARV.dial :air1 @02:50:39.00 adding ARV air @02:50:39.02 delay 2 seconds @02:50:41.10 AS.seek 0.536ml @02:50:47.61 delay 5 seconds @02:50:52.63 ARV.dial :carrier @02:50:53.05 delay 7 seconds @02:51:00.14 AS.seek 0.594ml @02:51:58.81 delay 5 seconds @02:52:03.83 ATV.dial :ASV @02:52:04.22 delay 2 seconds @02:52:06.29 AS.seek 0.6ml @02:52:12.80 delay 4 seconds @02:52:16.82 ATV.dial ARV @02:52:17.36 delay 2 seconds @02:52:19.38 ARV.dial :carrier @02:52:19.40 delay 2 seconds @02:52:21.47 AS.seek 0.65ml @02:53:12.02 delay 4 seconds @02:53:16.04 ATV.dial PCR @02:53:16.64 ASV.dial :waste1 @02:53:16.65 ARV.dial :air1 @02:53:17.09 Positioning Slug by 0.324ml @02:53:17.18 AS.seek 0.326ml @02:58:43.27 delay 5 seconds @02:58:48.29 ATV.dial ARV @02:58:49.06 ASV.dial :PCRlock @02:58:49.44 delay 5 seconds @02:58:54.47 Running IPc ProfileIPc thermocycling @02:58:54.48 PCR.program ProfileIPc @02:58:54.64 ProfileIPc should complete in < 1:50:49 @02:58:54.66 PCR.run 2:23:31.25 @04:23:05.61 PCR.off @04:23:08.43 PCR for IPc completed -- closed /var/log/esp/IPc18nov2002h.pcr @04:23:08.44 Full MFB Decontamination @04:23:08.47 Decon extraction column and decon AIV @04:23:08.50 SPE.hold 2:46:40,55C @04:23:08.55 ASV.dial :SPEdelivery @04:23:09.21 delay 20 seconds @04:23:29.24 AFV.select :carrierSolution @04:23:29.35 delay 2 seconds @04:23:31.37 AS.reconfigure ASconfig @04:23:31.43 AS.seek 0.5ml @04:23:41.76 delay 1 second @04:23:42.78 AFV.select :holdingCoil @04:23:42.88 ATV.dial :air1 @04:23:44.81 AS.reconfigure ASslow1 @04:23:44.87 AS.seek 0.52ml @04:23:49.18 ATV.dial :ASV @04:23:50.97 ASV.dial :SPErecovery @04:23:51.66 AS.seek 0.62ml @04:24:12.04 ATV.dial :waste @04:24:13.91 AS.reconfigure ASnorm @04:24:13.97 AS.seek :empty @04:24:38.62 Session Ended @04:24:45.60 AFV.select :holdingCoil @04:24:45.61 ATV.dial :waste @04:24:45.63 AS.reconfigure ASconfig @04:24:45.67 AS.seek 0ml @04:24:45.96 AFV.select :carrierSolution @04:24:46.07 delay 2 seconds @04:24:48.09 AS.seek 0.5ml @04:25:17.12 delay 1 second @04:25:18.14 AFV.select :holdingCoil @04:25:18.25 ATV.dial :air3 @04:25:19.14 AS.reconfigure ASslow1 @04:25:19.18 AS.seek 0.52ml @04:25:23.47 ATV.dial :air3 @04:25:23.54 AS.seek 0.53ml @04:25:25.85 delay 2 seconds @04:25:27.87 ATV.dial :bleach @04:25:28.33 AS.seek 0.71ml @04:26:04.79 delay 3 seconds @04:26:07.81 ATV.dial :air3 @04:26:08.38 delay 3 seconds @04:26:11.45 AS.seek 0.735ml @04:26:16.75 delay 3 seconds @04:26:19.77 ATV.dial :bleach @04:26:20.39 AS.seek 0.72ml @04:26:23.69 delay 3 seconds @04:26:26.71 ATV.dial ASV @04:26:27.79 ASV.dial :SPErecovery @04:26:27.86 AS.seek 0.365ml @04:27:39.51 ATV.dial :waste @04:27:41.27 AS.reconfigure ASnorm @04:27:41.31 AS.seek :empty @04:28:00.05 ATV.dial :air3 @04:28:00.72 AS.reconfigure ASslow1 @04:28:00.76 AS.seek 0.009ml @04:28:03.12 delay 2 seconds @04:28:05.14 ATV.dial :bleach @04:28:05.60 AS.seek 0.579ml @04:30:00.47 delay 3 seconds @04:30:03.49 ATV.dial :air3 @04:30:04.05 delay 3 seconds @04:30:07.12 AS.seek 0.604ml @04:30:12.41 delay 3 seconds @04:30:15.43 ATV.dial :bleach @04:30:16.05 AS.seek 0.589ml @04:30:19.37 delay 3 seconds @04:30:22.39 ATV.dial :ASV @04:30:23.52 ASV.dial :wasteR @04:30:24.00 AS.seek 0ml @04:32:22.76 delay 1:00 @04:33:22.79 ATV.dial ASV @04:33:22.80 AFV.select :carrierSolution @04:33:22.91 delay 2 seconds @04:33:24.93 AS.reconfigure ASconfig @04:33:24.98 AS.seek 1ml @04:34:22.72 delay 1 second @04:34:23.74 AFV.select :holdingCoil @04:34:23.85 delay 1 second @04:34:24.86 ASV.dial :wasteR @04:34:25.37 AS.reconfigure ASslow1 @04:34:25.43 AS.seek 0ml @04:37:46.83 delay 10 seconds @04:37:56.86 ATV.dial :air3 @04:37:58.35 AS.seek 0.01ml @04:38:00.74 delay 2 seconds @04:38:02.76 ATV.dial :bleach @04:38:03.38 AS.seek 0.58ml @04:39:58.27 delay 3 seconds @04:40:01.29 ATV.dial :air3 @04:40:01.85 delay 3 seconds @04:40:04.93 AS.seek 0.605ml @04:40:10.22 delay 3 seconds @04:40:13.24 ATV.dial :bleach @04:40:13.85 AS.seek 0.59ml @04:40:17.15 delay 3 seconds @04:40:20.17 ATV.dial ASV @04:40:21.21 ASV.dial :SPEdelivery @04:40:21.58 AS.seek 0ml @04:42:20.51 delay 1:00 @04:43:20.54 AFV.select :carrierSolution @04:43:20.65 delay 2 seconds @04:43:22.67 AS.reconfigure ASconfig @04:43:22.72 AS.seek 1ml @04:44:20.48 delay 1 second @04:44:21.50 AFV.select :holdingCoil @04:44:21.61 delay 1 second @04:44:22.62 ATV.dial ASV @04:44:22.63 ASV.dial :SPEdelivery @04:44:22.65 AS.reconfigure ASslow1 @04:44:22.70 AS.seek 0ml @04:47:44.19 delay 10 seconds @04:47:54.22 ATV.dial :air2 @04:47:55.06 AS.reconfigure ASslow0 @04:47:55.10 AS.seek 0.8ml @04:49:15.88 delay 3 seconds @04:49:18.90 ATV.dial :ASV @04:49:19.88 ASV.dial :SPEdelivery @04:49:19.90 AS.reconfigure ASslow1 @04:49:19.94 AS.seek 0.03ml @04:51:55.07 delay 20 seconds @04:52:15.10 ATV.dial :waste,via: :air3 @04:52:16.80 AS.reconfigure ASconfig @04:52:16.85 AS.seek :empty @04:52:18.91 SPE.coast @04:52:18.98 Removing Bleach from Mixing Coil @04:52:18.99 AFV.select :carrierSolution @04:52:19.09 delay 2 seconds @04:52:21.11 AS.seek 0.5ml @04:52:50.23 delay 1 second @04:52:51.25 AFV.select :holdingCoil @04:52:51.35 ATV.dial :ASV @04:52:53.02 ASV.dial :SPErecovery @04:52:53.65 AS.reconfigure ASslow1 @04:52:53.69 AS.seek 0.73ml @04:53:40.21 ASV.dial :wasteD @04:53:40.80 AS.reconfigure ASnorm @04:53:40.84 AS.seek 0ml @04:54:17.94 AFV.select :carrierSolution @04:54:18.05 delay 2 seconds @04:54:20.07 AS.reconfigure ASconfig @04:54:20.12 AS.seek 0.5ml @04:54:49.18 delay 1 second @04:54:50.20 AFV.select :holdingCoil @04:54:50.30 ATV.dial ASV @04:54:50.32 ASV.dial :SPErecovery @04:54:50.96 AS.reconfigure ASslow0 @04:54:51.00 AS.seek 0.3ml @04:55:11.41 ATV.dial :air2 @04:55:12.43 AS.reconfigure ASslow1 @04:55:12.47 AS.seek 0.32ml @04:55:16.83 ATV.dial ASV @04:55:17.85 AS.reconfigure ASslow0 @04:55:17.89 AS.seek 0.57ml @04:55:43.34 ASV.dial :wasteD @04:55:43.92 AS.reconfigure ASnorm @04:55:43.96 AS.seek 0ml @04:56:13.02 AFV.select :carrierSolution @04:56:13.13 delay 2 seconds @04:56:15.15 AS.reconfigure ASconfig @04:56:15.20 AS.seek 0.5ml @04:56:44.26 delay 1 second @04:56:45.28 AFV.select :holdingCoil @04:56:45.38 ATV.dial ASV @04:56:45.40 ASV.dial :SPErecovery @04:56:46.04 AS.reconfigure ASslow0 @04:56:46.08 AS.seek 0.3ml @04:57:06.49 ATV.dial :air2 @04:57:07.50 AS.reconfigure ASslow1 @04:57:07.54 AS.seek 0.32ml @04:57:11.89 ATV.dial ASV @04:57:13.01 AS.reconfigure ASslow0 @04:57:13.06 AS.seek 0.57ml @04:57:38.44 ASV.dial :wasteD @04:57:39.11 AS.reconfigure ASnorm @04:57:39.15 AS.seek 0ml @04:58:08.21 ATV.dial :air2 @04:58:09.17 AS.reconfigure ASslow0 @04:58:09.21 AS.seek 0.3ml @04:58:39.76 ATV.dial :ASV @04:58:40.73 ASV.dial :wasteD @04:58:40.75 AS.reconfigure ASconfig @04:58:40.79 AS.seek 0.05ml @04:58:55.48 ATV.dial :waste @04:58:57.31 AS.seek 0ml @04:59:00.50 ASV.dial :SPEdelivery @04:59:01.48 deconLysateINT @04:59:01.49 ATV.dial :air3 @04:59:02.15 AS.reconfigure ASslow1 @04:59:02.20 AS.seek 0.01ml @04:59:04.52 delay 2 seconds @04:59:06.54 ATV.dial :bleach @04:59:06.97 AS.seek 0.96ml @05:02:18.29 delay 3 seconds @05:02:21.31 ATV.dial :air3 @05:02:21.85 delay 3 seconds @05:02:24.93 AS.seek 0.985ml @05:02:30.27 delay 3 seconds @05:02:33.29 ATV.dial :bleach @05:02:33.90 AS.seek 0.97ml @05:02:37.32 delay 3 seconds @05:02:40.34 ATV.dial AIV @05:02:41.57 AIV.dial :coilToMFB @05:02:41.58 AWV.select :waste @05:02:41.59 AFV.select :carrierSolution @05:02:41.70 delay 2 seconds @05:02:43.72 AS.reconfigure ASconfig @05:02:43.77 AS.seek 1ml @05:02:45.77 delay 1 second @05:02:46.79 AFV.select :holdingCoil @05:02:46.90 delay 1 second @05:02:47.92 AS.reconfigure ASslow0 @05:02:47.96 AS.seek :empty @05:04:28.95 AFV.select :carrierSolution @05:04:29.06 delay 2 seconds @05:04:31.08 AS.reconfigure ASconfig @05:04:31.13 AS.seek 1ml @05:05:28.93 delay 1 second @05:05:29.95 AFV.select :holdingCoil @05:05:30.06 delay 1 second @05:05:31.08 AS.reconfigure ASslow0 @05:05:31.12 AS.seek :empty @05:07:12.12 AFV.select :carrierSolution @05:07:12.23 delay 2 seconds @05:07:14.25 AS.reconfigure ASconfig @05:07:14.30 AS.seek 1ml @05:08:12.10 delay 1 second @05:08:13.12 AFV.select :holdingCoil @05:08:13.23 delay 1 second @05:08:14.25 AS.reconfigure ASslow0 @05:08:14.29 AS.seek :empty @05:09:55.24 ATV.dial :air2 @05:09:55.90 AS.reconfigure ASslow1 @05:09:55.95 AS.seek 0.009ml @05:09:58.24 AS.reconfigure ASslow0 @05:09:58.29 AS.seek 1ml @05:11:38.20 ATV.dial AIV @05:11:38.97 AS.seek 0.02ml @05:13:17.87 ATV.dial :waste @05:13:19.50 AS.seek 0ml @05:13:21.77 AWV.select :air @05:13:21.92 Cleaning PCR @05:13:21.93 AFV.select :carrierSolution @05:13:22.03 delay 2 seconds @05:13:24.05 AS.reconfigure ASconfig @05:13:24.10 AS.seek 0.5ml @05:13:53.13 delay 1 second @05:13:54.15 AFV.select :holdingCoil @05:13:54.25 ASV.dial :waste1 @05:13:54.62 ATV.dial :air3 @05:13:55.27 AS.reconfigure ASslow1 @05:13:55.31 AS.seek 0.51ml @05:13:57.59 delay 2 seconds @05:13:59.61 ATV.dial :bleach @05:14:00.07 AS.seek 0.81ml @05:15:00.70 delay 3 seconds @05:15:03.72 ATV.dial :air3 @05:15:04.27 delay 3 seconds @05:15:07.34 AS.seek 0.835ml @05:15:12.63 delay 3 seconds @05:15:15.65 ATV.dial :bleach @05:15:16.77 AS.seek 0.82ml @05:15:20.10 delay 3 seconds @05:15:23.12 ARV.dial :waste @05:15:23.74 delay 3 seconds @05:15:26.76 ATV.dial :ARV @05:15:27.62 AS.reconfigure ASconfig @05:15:27.67 AS.seek 0.47ml @05:15:48.07 AFV.select :carrierSolution @05:15:48.18 delay 2 seconds @05:15:50.20 AS.seek 1ml @05:16:20.96 delay 1 second @05:16:21.98 AFV.select :holdingCoil @05:16:22.09 delay 1 second @05:16:23.11 AS.reconfigure ASfast @05:16:23.16 AS.seek 0ml @05:16:50.52 AFV.select :carrierSolution @05:16:50.63 delay 2 seconds @05:16:52.65 AS.reconfigure ASconfig @05:16:52.70 AS.seek 1ml @05:17:50.48 delay 1 second @05:17:51.50 AFV.select :holdingCoil @05:17:51.61 delay 1 second @05:17:52.63 AS.reconfigure ASfast @05:17:52.68 AS.seek 0ml @05:18:20.04 AFV.select :carrierSolution @05:18:20.15 delay 2 seconds @05:18:22.17 AS.reconfigure ASconfig @05:18:22.22 AS.seek 1ml @05:19:20.04 delay 1 second @05:19:21.06 AFV.select :holdingCoil @05:19:21.17 delay 1 second @05:19:22.19 AS.reconfigure ASfast @05:19:22.24 AS.seek 0ml @05:19:49.60 ARV.dial between carrier and air1 @05:19:50.10 ATV.dial :air3 @05:19:51.37 AS.reconfigure ASslow1 @05:19:51.42 AS.seek 0.01ml @05:19:53.73 delay 2 seconds @05:19:55.75 ATV.dial :bleach @05:19:56.35 AS.seek 0.81ml @05:22:37.49 delay 3 seconds @05:22:40.51 ATV.dial :air3 @05:22:41.06 delay 3 seconds @05:22:44.13 AS.seek 0.835ml @05:22:49.41 delay 3 seconds @05:22:52.43 ATV.dial :bleach @05:22:53.04 AS.seek 0.82ml @05:22:56.32 delay 3 seconds @05:22:59.34 ATV.dial PCR @05:22:59.73 AS.reconfigure ASconfig @05:22:59.78 AS.seek :empty @05:23:47.23 PCR.program :bleachProfile @05:23:47.28 PCR program should complete in < 2:45 @05:23:47.29 PCR.run 10:00 @05:26:49.10 PCR.off @05:26:49.13 AFV.select :carrierSolution @05:26:49.24 delay 2 seconds @05:26:51.26 AS.seek 1ml @05:27:49.04 delay 1 second @05:27:50.06 AFV.select :holdingCoil @05:27:50.17 delay 1 second @05:27:51.19 AS.reconfigure ASfast @05:27:51.24 AS.seek 0ml @05:28:18.57 delay 3 seconds @05:28:21.59 AFV.select :carrierSolution @05:28:21.70 delay 2 seconds @05:28:23.72 AS.reconfigure ASconfig @05:28:23.78 AS.seek 1ml @05:29:21.56 delay 1 second @05:29:22.58 AFV.select :holdingCoil @05:29:22.69 delay 1 second @05:29:23.71 AS.reconfigure ASfast @05:29:23.76 AS.seek 0ml @05:29:51.10 delay 3 seconds @05:29:54.12 AFV.select :carrierSolution @05:29:54.23 delay 2 seconds @05:29:56.25 AS.reconfigure ASconfig @05:29:56.30 AS.seek 1ml @05:30:54.12 delay 1 second @05:30:55.14 AFV.select :holdingCoil @05:30:55.25 delay 1 second @05:30:56.27 AS.reconfigure ASfast @05:30:56.32 AS.seek 0ml @05:31:23.68 delay 3 seconds @05:31:26.70 AFV.select :carrierSolution @05:31:26.81 delay 2 seconds @05:31:28.83 AS.reconfigure ASconfig @05:31:28.89 AS.seek 1ml @05:32:26.67 delay 1 second @05:32:27.69 AFV.select :holdingCoil @05:32:27.80 delay 1 second @05:32:28.82 AS.reconfigure ASfast @05:32:28.87 AS.seek 0ml @05:32:56.21 delay 3 seconds @05:32:59.23 AFV.select :carrierSolution @05:32:59.34 delay 2 seconds @05:33:01.36 AS.reconfigure ASconfig @05:33:01.41 AS.seek 1ml @05:33:59.13 delay 1 second @05:34:00.15 AFV.select :holdingCoil @05:34:00.26 delay 1 second @05:34:01.79 AS.reconfigure ASnorm @05:34:01.84 AS.seek 0ml @05:34:52.55 AFV.select :carrierSolution @05:34:52.66 delay 2 seconds @05:34:54.68 AS.reconfigure ASconfig @05:34:54.73 AS.seek 1ml @05:35:52.51 delay 1 second @05:35:53.53 AFV.select :holdingCoil @05:35:53.64 delay 1 second @05:35:54.65 ATV.dial :waste @05:35:55.86 AS.reconfigure ASnorm @05:35:55.90 AS.seek 0.5ml @05:36:21.46 ATV.dial :waste2 @05:36:22.72 AS.seek 0ml @05:36:48.21 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118nov2005h.pcr @05:36:48.23 Building PCR Slug from elutionWater with MM2 mix, PP4 primer @05:36:48.24 Priming with carrier @05:36:48.25 ATV.dial ARV @05:36:49.15 ARV.dial :carrier @05:36:49.49 AS.seek 0.5ml @05:37:15.09 AFV.select :carrierSolution @05:37:15.20 delay 2 seconds @05:37:17.22 AS.reconfigure ASconfig @05:37:17.27 AS.seek 1ml @05:37:46.27 delay 1 second @05:37:47.29 AFV.select :holdingCoil @05:37:47.40 delay 1 second @05:37:48.41 ATV.dial :waste @05:37:49.91 AS.reconfigure ASnorm @05:37:49.95 AS.seek :empty @05:38:40.68 AFV.select :carrierSolution @05:38:40.79 delay 2 seconds @05:38:42.81 AS.reconfigure ASconfig @05:38:42.86 AS.seek 0.5ml @05:39:11.98 delay 1 second @05:39:13.00 AFV.select :holdingCoil @05:39:13.11 Building Slug @05:39:13.12 ATV.dial :ARV @05:39:14.73 delay 2 seconds @05:39:16.75 ARV.dial :air1 @05:39:17.14 delay 2 seconds @05:39:19.21 AS.reconfigure ASslow2 @05:39:19.26 AS.seek 0.506ml @05:39:25.49 delay 2 seconds @05:39:27.51 ARV.dial :MM2 @05:39:29.01 delay 2 seconds @05:39:31.04 Adding MM2 @05:39:31.11 AS.seek 0.524ml @05:39:49.35 delay 25 seconds @05:40:14.37 ARV.dial :PP4 @05:40:14.76 Adding PP4 @05:40:14.77 delay 2 seconds @05:40:16.84 AS.seek 0.53ml @05:40:23.12 delay 10 seconds @05:40:33.15 ARV.dial :air3 @05:40:33.54 adding ARV air @05:40:33.55 delay 2 seconds @05:40:35.62 AS.seek 0.536ml @05:40:41.96 delay 5 seconds @05:40:46.98 ARV.dial :carrier @05:40:48.28 delay 7 seconds @05:40:55.37 AS.seek 0.594ml @05:41:53.95 delay 5 seconds @05:41:58.97 ATV.dial :elutionWater @05:42:00.42 delay 2 seconds @05:42:02.50 AS.seek 0.6ml @05:42:08.95 delay 4 seconds @05:42:12.97 ATV.dial ARV @05:42:14.37 delay 2 seconds @05:42:16.39 ARV.dial :carrier @05:42:16.40 delay 2 seconds @05:42:18.48 AS.seek 0.65ml @05:43:08.93 delay 4 seconds @05:43:12.95 ATV.dial PCR @05:43:13.70 ASV.dial :waste1 @05:43:13.71 ARV.dial :air1 @05:43:14.11 Positioning Slug by 0.324ml @05:43:14.19 AS.seek 0.326ml @05:48:40.21 delay 5 seconds @05:48:45.23 ATV.dial ARV @05:48:45.99 ASV.dial :PCRlock @05:48:46.37 delay 5 seconds @05:48:51.40 Running Tt1 Profile60C300 thermocycling @05:48:51.41 PCR.program Profile60C300 @05:48:51.53 Profile60C300 should complete in < 1:45:21 @05:48:51.55 PCR.run 2:16:41.25 @06:46:20.33 Session Begun @06:46:29.43 -> showlog 10 @06:48:08.15 -> exit @06:48:08.16 Session Ended @06:50:08.93 Session Begun @06:50:14.05 -> showlog 10 @07:10:54.99 PCR.off @07:10:56.52 PCR for Tt1 completed -- closed /var/log/esp/Tt118nov2005h.pcr @07:10:56.60 Cleaning PCR @07:10:56.61 AFV.select :carrierSolution @07:10:56.74 delay 2 seconds @07:10:58.76 AS.reconfigure ASconfig @07:10:58.83 AS.seek 0.5ml @07:11:09.14 delay 1 second @07:11:10.16 AFV.select :holdingCoil @07:11:10.26 ASV.dial :waste1 @07:11:10.66 ATV.dial :air3 @07:11:12.47 AS.reconfigure ASslow1 @07:11:12.53 AS.seek 0.51ml @07:11:14.81 delay 2 seconds @07:11:16.83 ATV.dial :bleach @07:11:17.45 AS.seek 0.81ml @07:12:18.09 delay 3 seconds @07:12:21.11 ATV.dial :air3 @07:12:21.67 delay 3 seconds @07:12:24.75 AS.seek 0.835ml @07:12:30.06 delay 3 seconds @07:12:33.08 ATV.dial :bleach @07:12:33.70 AS.seek 0.82ml @07:12:36.97 delay 3 seconds @07:12:39.99 ARV.dial :waste @07:12:40.62 delay 3 seconds @07:12:43.64 ATV.dial :ARV @07:12:44.53 AS.reconfigure ASconfig @07:12:44.59 AS.seek 0.47ml @07:13:04.98 AFV.select :carrierSolution @07:13:05.10 delay 2 seconds @07:13:07.12 AS.seek 1ml @07:13:37.89 delay 1 second @07:13:38.91 AFV.select :holdingCoil @07:13:39.02 delay 1 second @07:13:40.04 AS.reconfigure ASfast @07:13:40.11 AS.seek 0ml @07:14:07.47 AFV.select :carrierSolution @07:14:07.59 delay 2 seconds @07:14:09.61 AS.reconfigure ASconfig @07:14:09.67 AS.seek 1ml @07:15:07.47 delay 1 second @07:15:08.49 AFV.select :holdingCoil @07:15:08.60 delay 1 second @07:15:09.62 AS.reconfigure ASfast @07:15:09.69 AS.seek 0ml @07:15:37.06 AFV.select :carrierSolution @07:15:37.18 delay 2 seconds @07:15:39.20 AS.reconfigure ASconfig @07:15:39.26 AS.seek 1ml @07:16:37.06 delay 1 second @07:16:38.08 AFV.select :holdingCoil @07:16:38.19 delay 1 second @07:16:39.21 AS.reconfigure ASfast @07:16:39.28 AS.seek 0ml @07:17:06.64 ARV.dial between carrier and air1 @07:17:07.16 ATV.dial :air3 @07:17:08.45 AS.reconfigure ASslow1 @07:17:08.51 AS.seek 0.01ml @07:17:10.84 delay 2 seconds @07:17:12.86 ATV.dial :bleach @07:17:13.48 AS.seek 0.81ml @07:19:54.59 delay 3 seconds @07:19:57.61 ATV.dial :air3 @07:19:58.17 delay 3 seconds @07:20:01.24 AS.seek 0.835ml @07:20:06.55 delay 3 seconds @07:20:09.57 ATV.dial :bleach @07:20:10.18 AS.seek 0.82ml @07:20:13.48 delay 3 seconds @07:20:16.50 ATV.dial PCR @07:20:16.91 AS.reconfigure ASconfig @07:20:16.97 AS.seek :empty @07:21:04.44 PCR.program :bleachProfile @07:21:04.50 PCR program should complete in < 2:45 @07:21:04.51 PCR.run 10:00 @07:24:03.31 PCR.off @07:24:03.35 AFV.select :carrierSolution @07:24:03.46 delay 2 seconds @07:24:05.48 AS.seek 1ml @07:25:03.36 delay 1 second @07:25:04.38 AFV.select :holdingCoil @07:25:04.49 delay 1 second @07:25:05.51 AS.reconfigure ASfast @07:25:05.58 AS.seek 0ml @07:25:32.95 delay 3 seconds @07:25:35.97 AFV.select :carrierSolution @07:25:36.08 delay 2 seconds @07:25:38.10 AS.reconfigure ASconfig @07:25:38.17 AS.seek 1ml @07:26:35.94 delay 1 second @07:26:36.96 AFV.select :holdingCoil @07:26:37.07 delay 1 second @07:26:38.09 AS.reconfigure ASfast @07:26:38.15 AS.seek 0ml @07:27:05.50 delay 3 seconds @07:27:08.52 AFV.select :carrierSolution @07:27:08.63 delay 2 seconds @07:27:10.65 AS.reconfigure ASconfig @07:27:10.71 AS.seek 1ml @07:28:08.52 delay 1 second @07:28:09.54 AFV.select :holdingCoil @07:28:09.65 delay 1 second @07:28:11.12 AS.reconfigure ASfast @07:28:11.24 AS.seek 0ml @07:28:38.58 delay 3 seconds @07:28:41.60 AFV.select :carrierSolution @07:28:41.71 delay 2 seconds @07:28:43.73 AS.reconfigure ASconfig @07:28:43.80 AS.seek 1ml @07:29:41.59 delay 1 second @07:29:42.61 AFV.select :holdingCoil @07:29:42.72 delay 1 second @07:29:43.74 AS.reconfigure ASfast @07:29:43.80 AS.seek 0ml @07:30:11.16 delay 3 seconds @07:30:14.18 AFV.select :carrierSolution @07:30:14.29 delay 2 seconds @07:30:16.31 AS.reconfigure ASconfig @07:30:16.37 AS.seek 1ml @07:31:14.16 delay 1 second @07:31:15.18 AFV.select :holdingCoil @07:31:15.29 delay 1 second @07:31:16.31 AS.reconfigure ASnorm @07:31:16.37 AS.seek 0ml @07:32:07.11 Setting up PCR for Rp -- writing /var/log/esp/Rp18nov2007h.pcr @07:32:07.13 Building PCR Slug from elutionWater with MM2 mix, PP3 primer @07:32:07.15 Priming with carrier @07:32:07.16 ATV.dial ARV @07:32:07.78 ARV.dial :carrier @07:32:08.15 AS.seek 0.5ml @07:32:33.69 AFV.select :carrierSolution @07:32:33.82 delay 2 seconds @07:32:35.84 AS.reconfigure ASconfig @07:32:35.91 AS.seek 1ml @07:33:04.94 delay 1 second @07:33:05.96 AFV.select :holdingCoil @07:33:06.07 delay 1 second @07:33:07.08 ATV.dial :waste @07:33:08.73 AS.reconfigure ASnorm @07:33:08.80 AS.seek :empty @07:33:59.56 AFV.select :carrierSolution @07:33:59.69 delay 2 seconds @07:34:01.71 AS.reconfigure ASconfig @07:34:01.77 AS.seek 0.5ml @07:34:30.89 delay 1 second @07:34:31.91 AFV.select :holdingCoil @07:34:32.03 Building Slug @07:34:32.04 ATV.dial :ARV @07:34:33.67 delay 2 seconds @07:34:35.69 ARV.dial :air1 @07:34:36.12 delay 2 seconds @07:34:38.20 AS.reconfigure ASslow2 @07:34:38.26 AS.seek 0.506ml @07:34:44.55 delay 2 seconds @07:34:46.57 ARV.dial :MM2 @07:34:48.08 delay 2 seconds @07:34:50.11 Adding MM2 @07:34:50.20 AS.seek 0.524ml @07:35:08.42 delay 25 seconds @07:35:33.45 ARV.dial :PP3 @07:35:33.84 Adding PP3 @07:35:33.86 delay 2 seconds @07:35:35.93 AS.seek 0.53ml @07:35:42.39 delay 10 seconds @07:35:52.42 ARV.dial :air2 @07:35:52.83 adding ARV air @07:35:52.84 delay 2 seconds @07:35:54.93 AS.seek 0.536ml @07:36:01.17 delay 5 seconds @07:36:06.19 ARV.dial :carrier @07:36:07.50 delay 7 seconds @07:36:14.58 AS.seek 0.594ml @07:37:13.09 delay 5 seconds @07:37:18.11 ATV.dial :elutionWater @07:37:19.53 delay 2 seconds @07:37:21.61 AS.seek 0.6ml @07:37:27.98 delay 4 seconds @07:37:32.00 ATV.dial ARV @07:37:33.41 delay 2 seconds @07:37:35.43 ARV.dial :carrier @07:37:35.45 delay 2 seconds @07:37:37.53 AS.seek 0.65ml @07:38:28.16 delay 4 seconds @07:38:32.18 ATV.dial PCR @07:38:32.94 ASV.dial :waste1 @07:38:32.95 ARV.dial :air1 @07:38:33.38 Positioning Slug by 0.324ml @07:38:33.48 AS.seek 0.326ml @07:43:59.48 delay 5 seconds @07:44:04.50 ATV.dial ARV @07:44:05.28 ASV.dial :PCRlock @07:44:05.69 delay 5 seconds @07:44:10.72 Running Rp Profile60C300 thermocycling @07:44:10.73 PCR.program Profile60C300 @07:44:10.88 Profile60C300 should complete in < 1:45:21 @07:44:10.89 PCR.run 2:16:41.25 @08:28:15.49 -> exit @08:28:15.51 Session Ended @08:30:36.56 Session Begun @08:30:41.80 -> showlog 10 @09:05:43.81 -> showlog 10 @09:06:11.56 PCR.off @09:06:13.09 PCR for Rp completed -- closed /var/log/esp/Rp18nov2007h.pcr @09:06:13.17 Cleaning PCR @09:06:13.18 AFV.select :carrierSolution @09:06:13.31 delay 2 seconds @09:06:15.33 AS.reconfigure ASconfig @09:06:15.39 AS.seek 0.5ml @09:06:25.69 delay 1 second @09:06:26.71 AFV.select :holdingCoil @09:06:26.81 ASV.dial :waste1 @09:06:27.30 ATV.dial :air3 @09:06:28.58 AS.reconfigure ASslow1 @09:06:28.64 AS.seek 0.51ml @09:06:30.94 delay 2 seconds @09:06:32.96 ATV.dial :bleach @09:06:33.58 AS.seek 0.81ml @09:07:34.18 delay 3 seconds @09:07:37.20 ATV.dial :air3 @09:07:38.29 delay 3 seconds @09:07:41.36 AS.seek 0.835ml @09:07:46.77 delay 3 seconds @09:07:49.79 ATV.dial :bleach @09:07:50.41 AS.seek 0.82ml @09:07:53.71 delay 3 seconds @09:07:56.73 ARV.dial :waste @09:07:57.38 delay 3 seconds @09:08:00.40 ATV.dial :ARV @09:08:01.28 AS.reconfigure ASconfig @09:08:01.34 AS.seek 0.47ml @09:08:21.74 AFV.select :carrierSolution @09:08:21.86 delay 2 seconds @09:08:23.88 AS.seek 1ml @09:08:54.66 delay 1 second @09:08:55.68 AFV.select :holdingCoil @09:08:55.79 delay 1 second @09:08:56.81 AS.reconfigure ASfast @09:08:56.88 AS.seek 0ml @09:09:24.27 AFV.select :carrierSolution @09:09:24.40 delay 2 seconds @09:09:26.42 AS.reconfigure ASconfig @09:09:26.48 AS.seek 1ml @09:10:24.27 delay 1 second @09:10:25.29 AFV.select :holdingCoil @09:10:25.40 delay 1 second @09:10:26.42 AS.reconfigure ASfast @09:10:26.49 AS.seek 0ml @09:10:53.85 AFV.select :carrierSolution @09:10:53.97 delay 2 seconds @09:10:55.99 AS.reconfigure ASconfig @09:10:56.05 AS.seek 1ml @09:11:53.85 delay 1 second @09:11:54.87 AFV.select :holdingCoil @09:11:54.98 delay 1 second @09:11:56.00 AS.reconfigure ASfast @09:11:56.07 AS.seek 0ml @09:12:23.41 ARV.dial between carrier and air1 @09:12:23.93 ATV.dial :air3 @09:12:25.22 AS.reconfigure ASslow1 @09:12:25.28 AS.seek 0.01ml @09:12:27.72 delay 2 seconds @09:12:29.74 ATV.dial :bleach @09:12:30.36 AS.seek 0.81ml @09:15:11.52 delay 3 seconds @09:15:14.54 ATV.dial :air3 @09:15:15.11 delay 3 seconds @09:15:18.18 AS.seek 0.835ml @09:15:23.49 delay 3 seconds @09:15:26.51 ATV.dial :bleach @09:15:27.13 AS.seek 0.82ml @09:15:30.44 delay 3 seconds @09:15:33.46 ATV.dial PCR @09:15:33.86 AS.reconfigure ASconfig @09:15:33.92 AS.seek :empty @09:16:21.40 PCR.program :bleachProfile @09:16:21.46 PCR program should complete in < 2:45 @09:16:21.47 PCR.run 10:00 @09:19:20.28 PCR.off @09:19:20.32 AFV.select :carrierSolution @09:19:20.44 delay 2 seconds @09:19:22.46 AS.seek 1ml @09:20:20.30 delay 1 second @09:20:21.32 AFV.select :holdingCoil @09:20:21.43 delay 1 second @09:20:22.45 AS.reconfigure ASfast @09:20:22.51 AS.seek 0ml @09:20:49.88 delay 3 seconds @09:20:52.90 AFV.select :carrierSolution @09:20:53.01 delay 2 seconds @09:20:55.03 AS.reconfigure ASconfig @09:20:55.09 AS.seek 1ml @09:21:52.90 delay 1 second @09:21:53.92 AFV.select :holdingCoil @09:21:54.03 delay 1 second @09:21:55.05 AS.reconfigure ASfast @09:21:55.11 AS.seek 0ml @09:22:22.46 delay 3 seconds @09:22:25.48 AFV.select :carrierSolution @09:22:25.59 delay 2 seconds @09:22:27.61 AS.reconfigure ASconfig @09:22:27.68 AS.seek 1ml @09:23:25.47 delay 1 second @09:23:26.49 AFV.select :holdingCoil @09:23:26.60 delay 1 second @09:23:27.62 AS.reconfigure ASfast @09:23:27.68 AS.seek 0ml @09:23:55.04 delay 3 seconds @09:23:58.06 AFV.select :carrierSolution @09:23:58.17 delay 2 seconds @09:24:00.19 AS.reconfigure ASconfig @09:24:00.25 AS.seek 1ml @09:24:58.05 delay 1 second @09:24:59.07 AFV.select :holdingCoil @09:24:59.18 delay 1 second @09:25:00.20 AS.reconfigure ASfast @09:25:00.26 AS.seek 0ml @09:25:28.13 delay 3 seconds @09:25:31.15 AFV.select :carrierSolution @09:25:31.26 delay 2 seconds @09:25:33.28 AS.reconfigure ASconfig @09:25:33.36 AS.seek 1ml @09:26:31.13 delay 1 second @09:26:32.15 AFV.select :holdingCoil @09:26:32.26 delay 1 second @09:26:33.28 AS.reconfigure ASnorm @09:26:33.35 AS.seek 0ml @09:27:24.09 Setting up PCR for Bb -- writing /var/log/esp/Bb18nov2009h.pcr @09:27:24.11 Building PCR Slug from elutionWater with MM3 mix, PP5 primer @09:27:24.13 Priming with carrier @09:27:24.14 ATV.dial ARV @09:27:24.77 ARV.dial :carrier @09:27:25.14 AS.seek 0.5ml @09:27:50.72 AFV.select :carrierSolution @09:27:50.85 delay 2 seconds @09:27:52.87 AS.reconfigure ASconfig @09:27:52.94 AS.seek 1ml @09:28:21.97 delay 1 second @09:28:22.99 AFV.select :holdingCoil @09:28:23.10 delay 1 second @09:28:24.11 ATV.dial :waste @09:28:25.77 AS.reconfigure ASnorm @09:28:25.83 AS.seek :empty @09:29:16.58 AFV.select :carrierSolution @09:29:16.71 delay 2 seconds @09:29:18.73 AS.reconfigure ASconfig @09:29:18.79 AS.seek 0.5ml @09:29:47.91 delay 1 second @09:29:48.93 AFV.select :holdingCoil @09:29:49.04 Building Slug @09:29:49.05 ATV.dial :ARV @09:29:50.69 delay 2 seconds @09:29:52.71 ARV.dial :air1 @09:29:53.13 delay 2 seconds @09:29:55.21 AS.reconfigure ASslow2 @09:29:55.28 AS.seek 0.506ml @09:30:01.46 delay 2 seconds @09:30:03.48 ARV.dial :MM3 @09:30:04.55 delay 2 seconds @09:30:06.58 Adding MM3 @09:30:06.67 AS.seek 0.524ml @09:30:25.02 delay 25 seconds @09:30:50.04 ARV.dial :PP5 @09:30:50.44 Adding PP5 @09:30:50.46 delay 2 seconds @09:30:52.54 AS.seek 0.53ml @09:30:59.01 delay 10 seconds @09:31:09.04 ARV.dial :air3 @09:31:09.44 adding ARV air @09:31:09.46 delay 2 seconds @09:31:11.53 AS.seek 0.536ml @09:31:17.79 delay 5 seconds @09:31:22.81 ARV.dial :carrier @09:31:24.12 delay 7 seconds @09:31:31.20 AS.seek 0.594ml @09:32:29.87 delay 5 seconds @09:32:34.89 ATV.dial :elutionWater @09:32:36.33 delay 2 seconds @09:32:38.41 AS.seek 0.6ml @09:32:44.69 delay 4 seconds @09:32:48.71 ATV.dial ARV @09:32:50.24 delay 2 seconds @09:32:52.26 ARV.dial :carrier @09:32:52.28 delay 2 seconds @09:32:54.36 AS.seek 0.65ml @09:33:45.15 delay 4 seconds @09:33:49.17 ATV.dial PCR @09:33:49.93 ASV.dial :waste1 @09:33:49.94 ARV.dial :air1 @09:33:50.38 Positioning Slug by 0.324ml @09:33:50.47 AS.seek 0.326ml @09:39:16.33 delay 5 seconds @09:39:21.35 ATV.dial ARV @09:39:22.12 ASV.dial :PCRlock @09:39:22.52 delay 5 seconds @09:39:27.55 Running Bb Profile60C300 thermocycling @09:39:27.56 PCR.program Profile60C300 @09:39:27.70 Profile60C300 should complete in < 1:45:21 @09:39:27.72 PCR.run 2:16:41.25 @11:01:19.73 PCR.off @11:01:21.26 PCR for Bb completed -- closed /var/log/esp/Bb18nov2009h.pcr @11:01:21.33 Cleaning PCR @11:01:21.34 AFV.select :carrierSolution @11:01:21.47 delay 2 seconds @11:01:23.49 AS.reconfigure ASconfig @11:01:23.56 AS.seek 0.5ml @11:01:33.87 delay 1 second @11:01:34.89 AFV.select :holdingCoil @11:01:34.99 ASV.dial :waste1 @11:01:35.39 ATV.dial :air3 @11:01:36.68 AS.reconfigure ASslow1 @11:01:36.74 AS.seek 0.51ml @11:01:39.05 delay 2 seconds @11:01:41.07 ATV.dial :bleach @11:01:41.71 AS.seek 0.81ml @11:02:42.31 delay 3 seconds @11:02:45.33 ATV.dial :air3 @11:02:45.89 delay 3 seconds @11:02:48.96 AS.seek 0.835ml @11:02:54.27 delay 3 seconds @11:02:57.29 ATV.dial :bleach @11:02:57.92 AS.seek 0.82ml @11:03:01.23 delay 3 seconds @11:03:04.25 ARV.dial :waste @11:03:04.87 delay 3 seconds @11:03:07.89 ATV.dial :ARV @11:03:09.27 AS.reconfigure ASconfig @11:03:09.33 AS.seek 0.47ml @11:03:29.78 AFV.select :carrierSolution @11:03:29.90 delay 2 seconds @11:03:31.92 AS.seek 1ml @11:04:02.67 delay 1 second @11:04:03.69 AFV.select :holdingCoil @11:04:03.80 delay 1 second @11:04:04.82 AS.reconfigure ASfast @11:04:04.89 AS.seek 0ml @11:04:32.25 AFV.select :carrierSolution @11:04:32.37 delay 2 seconds @11:04:34.39 AS.reconfigure ASconfig @11:04:34.45 AS.seek 1ml @11:05:32.25 delay 1 second @11:05:33.27 AFV.select :holdingCoil @11:05:33.38 delay 1 second @11:05:34.40 AS.reconfigure ASfast @11:05:34.47 AS.seek 0ml @11:06:01.82 AFV.select :carrierSolution @11:06:01.94 delay 2 seconds @11:06:03.96 AS.reconfigure ASconfig @11:06:04.02 AS.seek 1ml @11:07:01.79 delay 1 second @11:07:02.81 AFV.select :holdingCoil @11:07:02.92 delay 1 second @11:07:03.94 AS.reconfigure ASfast @11:07:04.01 AS.seek 0ml @11:07:31.37 ARV.dial between carrier and air1 @11:07:31.89 ATV.dial :air3 @11:07:33.18 AS.reconfigure ASslow1 @11:07:33.24 AS.seek 0.01ml @11:07:35.61 delay 2 seconds @11:07:37.63 ATV.dial :bleach @11:07:38.26 AS.seek 0.81ml @11:10:19.40 delay 3 seconds @11:10:22.42 ATV.dial :air3 @11:10:22.98 delay 3 seconds @11:10:26.05 AS.seek 0.835ml @11:10:31.37 delay 3 seconds @11:10:34.39 ATV.dial :bleach @11:10:35.01 AS.seek 0.82ml @11:10:38.29 delay 3 seconds @11:10:41.31 ATV.dial PCR @11:10:41.72 AS.reconfigure ASconfig @11:10:41.78 AS.seek :empty @11:11:29.26 PCR.program :bleachProfile @11:11:29.32 PCR program should complete in < 2:45 @11:11:29.33 PCR.run 10:00 @11:14:28.12 PCR.off @11:14:28.16 AFV.select :carrierSolution @11:14:28.28 delay 2 seconds @11:14:30.30 AS.seek 1ml @11:15:28.14 delay 1 second @11:15:29.16 AFV.select :holdingCoil @11:15:29.27 delay 1 second @11:15:30.29 AS.reconfigure ASfast @11:15:30.35 AS.seek 0ml @11:15:57.68 delay 3 seconds @11:16:00.70 AFV.select :carrierSolution @11:16:00.81 delay 2 seconds @11:16:02.83 AS.reconfigure ASconfig @11:16:02.90 AS.seek 1ml @11:17:00.68 delay 1 second @11:17:01.70 AFV.select :holdingCoil @11:17:01.81 delay 1 second @11:17:02.83 AS.reconfigure ASfast @11:17:02.89 AS.seek 0ml @11:17:30.26 delay 3 seconds @11:17:33.28 AFV.select :carrierSolution @11:17:33.39 delay 2 seconds @11:17:35.41 AS.reconfigure ASconfig @11:17:35.48 AS.seek 1ml @11:18:33.26 delay 1 second @11:18:34.28 AFV.select :holdingCoil @11:18:34.39 delay 1 second @11:18:35.41 AS.reconfigure ASfast @11:18:35.47 AS.seek 0ml @11:19:02.81 delay 3 seconds @11:19:05.83 AFV.select :carrierSolution @11:19:05.94 delay 2 seconds @11:19:07.96 AS.reconfigure ASconfig @11:19:08.03 AS.seek 1ml @11:20:05.78 delay 1 second @11:20:06.80 AFV.select :holdingCoil @11:20:06.91 delay 1 second @11:20:07.93 AS.reconfigure ASfast @11:20:07.99 AS.seek 0ml @11:20:35.34 delay 3 seconds @11:20:38.36 AFV.select :carrierSolution @11:20:38.47 delay 2 seconds @11:20:40.49 AS.reconfigure ASconfig @11:20:40.55 AS.seek 1ml @11:21:38.29 delay 1 second @11:21:39.31 AFV.select :holdingCoil @11:21:39.42 delay 1 second @11:21:40.44 AS.reconfigure ASnorm @11:21:40.50 AS.seek 0ml @11:22:31.27 Setting up PCR for Ss -- writing /var/log/esp/Ss18nov2011h.pcr @11:22:31.29 Building PCR Slug from elutionWater with MM1 mix, PP2 primer @11:22:31.32 Priming with carrier @11:22:31.33 ATV.dial ARV @11:22:31.95 ARV.dial :carrier @11:22:32.82 AS.seek 0.5ml @11:22:58.42 AFV.select :carrierSolution @11:22:58.55 delay 2 seconds @11:23:00.57 AS.reconfigure ASconfig @11:23:00.64 AS.seek 1ml @11:23:29.65 delay 1 second @11:23:30.67 AFV.select :holdingCoil @11:23:30.78 delay 1 second @11:23:31.79 ATV.dial :waste @11:23:33.57 AS.reconfigure ASnorm @11:23:33.63 AS.seek :empty @11:24:24.37 AFV.select :carrierSolution @11:24:24.50 delay 2 seconds @11:24:26.52 AS.reconfigure ASconfig @11:24:26.58 AS.seek 0.5ml @11:24:55.72 delay 1 second @11:24:56.74 AFV.select :holdingCoil @11:24:56.85 Building Slug @11:24:56.86 ATV.dial :ARV @11:24:58.50 delay 2 seconds @11:25:00.52 ARV.dial :air1 @11:25:00.93 delay 2 seconds @11:25:03.00 AS.reconfigure ASslow2 @11:25:03.07 AS.seek 0.506ml @11:25:09.42 delay 2 seconds @11:25:11.44 ARV.dial :MM1 @11:25:12.04 delay 2 seconds @11:25:14.07 Adding MM1 @11:25:14.15 AS.seek 0.524ml @11:25:32.62 delay 25 seconds @11:25:57.64 ARV.dial :PP2 @11:25:58.04 Adding PP2 @11:25:58.06 delay 2 seconds @11:26:00.14 AS.seek 0.53ml @11:26:06.37 delay 10 seconds @11:26:16.40 ARV.dial :air2 @11:26:16.81 adding ARV air @11:26:16.82 delay 2 seconds @11:26:18.90 AS.seek 0.536ml @11:26:25.08 delay 5 seconds @11:26:30.10 ARV.dial :carrier @11:26:31.40 delay 7 seconds @11:26:38.48 AS.seek 0.594ml @11:27:37.14 delay 5 seconds @11:27:42.16 ATV.dial :elutionWater @11:27:43.61 delay 2 seconds @11:27:45.69 AS.seek 0.6ml @11:27:52.12 delay 4 seconds @11:27:56.14 ATV.dial ARV @11:27:57.56 delay 2 seconds @11:27:59.58 ARV.dial :carrier @11:27:59.60 delay 2 seconds @11:28:01.67 AS.seek 0.65ml @11:28:52.26 delay 4 seconds @11:28:56.28 ATV.dial PCR @11:28:57.06 ASV.dial :waste1 @11:28:57.07 ARV.dial :air1 @11:28:57.50 Positioning Slug by 0.324ml @11:28:57.59 AS.seek 0.326ml @11:34:23.56 delay 5 seconds @11:34:28.58 ATV.dial ARV @11:34:29.48 ASV.dial :PCRlock @11:34:29.86 delay 5 seconds @11:34:34.89 Running Ss Profile60C350 thermocycling @11:34:34.90 PCR.program Profile60C350 @11:34:35.05 Profile60C350 should complete in < 1:48:05 @11:34:35.07 PCR.run 2:20:06.25 @12:56:50.03 PCR.off @12:56:52.04 PCR for Ss completed -- closed /var/log/esp/Ss18nov2011h.pcr @12:56:52.11 Cleaning PCR @12:56:52.12 AFV.select :carrierSolution @12:56:52.26 delay 2 seconds @12:56:54.28 AS.reconfigure ASconfig @12:56:54.34 AS.seek 0.5ml @12:57:04.64 delay 1 second @12:57:05.66 AFV.select :holdingCoil @12:57:05.76 ASV.dial :waste1 @12:57:06.14 ATV.dial :air3 @12:57:07.42 AS.reconfigure ASslow1 @12:57:07.48 AS.seek 0.51ml @12:57:09.76 delay 2 seconds @12:57:11.78 ATV.dial :bleach @12:57:12.41 AS.seek 0.81ml @12:58:13.02 delay 3 seconds @12:58:16.04 ATV.dial :air3 @12:58:16.60 delay 3 seconds @12:58:19.67 AS.seek 0.835ml @12:58:25.00 delay 3 seconds @12:58:28.02 ATV.dial :bleach @12:58:28.64 AS.seek 0.82ml @12:58:31.96 delay 3 seconds @12:58:34.98 ARV.dial :waste @12:58:35.63 delay 3 seconds @12:58:38.65 ATV.dial :ARV @12:58:40.14 AS.reconfigure ASconfig @12:58:40.20 AS.seek 0.47ml @12:59:00.63 AFV.select :carrierSolution @12:59:00.75 delay 2 seconds @12:59:02.77 AS.seek 1ml @12:59:33.50 delay 1 second @12:59:34.52 AFV.select :holdingCoil @12:59:34.63 delay 1 second @12:59:35.65 AS.reconfigure ASfast @12:59:35.72 AS.seek 0ml @13:00:03.08 AFV.select :carrierSolution @13:00:03.20 delay 2 seconds @13:00:05.22 AS.reconfigure ASconfig @13:00:05.28 AS.seek 1ml @13:01:03.04 delay 1 second @13:01:04.06 AFV.select :holdingCoil @13:01:04.17 delay 1 second @13:01:05.19 AS.reconfigure ASfast @13:01:05.26 AS.seek 0ml @13:01:32.61 AFV.select :carrierSolution @13:01:32.73 delay 2 seconds @13:01:34.75 AS.reconfigure ASconfig @13:01:34.81 AS.seek 1ml @13:02:32.57 delay 1 second @13:02:33.59 AFV.select :holdingCoil @13:02:33.70 delay 1 second @13:02:34.72 AS.reconfigure ASfast @13:02:34.79 AS.seek 0ml @13:03:02.13 ARV.dial between carrier and air1 @13:03:02.65 ATV.dial :air3 @13:03:03.94 AS.reconfigure ASslow1 @13:03:04.00 AS.seek 0.01ml @13:03:06.44 delay 2 seconds @13:03:08.46 ATV.dial :bleach @13:03:09.08 AS.seek 0.81ml @13:05:50.27 delay 3 seconds @13:05:53.29 ATV.dial :air3 @13:05:53.85 delay 3 seconds @13:05:56.92 AS.seek 0.835ml @13:06:02.26 delay 3 seconds @13:06:05.28 ATV.dial :bleach @13:06:05.91 AS.seek 0.82ml @13:06:09.24 delay 3 seconds @13:06:12.26 ATV.dial PCR @13:06:12.66 AS.reconfigure ASconfig @13:06:12.72 AS.seek :empty @13:07:00.21 PCR.program :bleachProfile @13:07:00.27 PCR program should complete in < 2:45 @13:07:00.28 PCR.run 10:00 @13:09:59.08 PCR.off @13:09:59.12 AFV.select :carrierSolution @13:09:59.24 delay 2 seconds @13:10:01.26 AS.seek 1ml @13:10:59.05 delay 1 second @13:11:00.07 AFV.select :holdingCoil @13:11:00.18 delay 1 second @13:11:01.20 AS.reconfigure ASfast @13:11:01.26 AS.seek 0ml @13:11:28.63 delay 3 seconds @13:11:31.65 AFV.select :carrierSolution @13:11:31.76 delay 2 seconds @13:11:33.78 AS.reconfigure ASconfig @13:11:33.85 AS.seek 1ml @13:12:31.65 delay 1 second @13:12:32.67 AFV.select :holdingCoil @13:12:32.78 delay 1 second @13:12:33.80 AS.reconfigure ASfast @13:12:33.86 AS.seek 0ml @13:13:01.22 delay 3 seconds @13:13:04.24 AFV.select :carrierSolution @13:13:04.35 delay 2 seconds @13:13:06.37 AS.reconfigure ASconfig @13:13:06.43 AS.seek 1ml @13:14:04.22 delay 1 second @13:14:05.24 AFV.select :holdingCoil @13:14:05.35 delay 1 second @13:14:06.37 AS.reconfigure ASfast @13:14:06.44 AS.seek 0ml @13:14:33.80 delay 3 seconds @13:14:36.82 AFV.select :carrierSolution @13:14:36.93 delay 2 seconds @13:14:38.95 AS.reconfigure ASconfig @13:14:39.03 AS.seek 1ml @13:15:36.82 delay 1 second @13:15:37.84 AFV.select :holdingCoil @13:15:37.95 delay 1 second @13:15:38.97 AS.reconfigure ASfast @13:15:39.03 AS.seek 0ml @13:16:06.38 delay 3 seconds @13:16:09.40 AFV.select :carrierSolution @13:16:09.51 delay 2 seconds @13:16:11.53 AS.reconfigure ASconfig @13:16:11.59 AS.seek 1ml @13:17:09.38 delay 1 second @13:17:10.40 AFV.select :holdingCoil @13:17:10.51 delay 1 second @13:17:11.53 AS.reconfigure ASnorm @13:17:11.59 AS.seek 0ml @13:18:02.32 Setting up PCR for IPc -- writing /var/log/esp/IPc18nov2013h.pcr @13:18:02.34 Building PCR Slug from elutionWater with MM1 mix, PP1 primer @13:18:02.37 Priming with carrier @13:18:02.39 ATV.dial ARV @13:18:03.63 ARV.dial :carrier @13:18:04.00 AS.seek 0.5ml @13:18:29.58 AFV.select :carrierSolution @13:18:29.71 delay 2 seconds @13:18:31.73 AS.reconfigure ASconfig @13:18:31.79 AS.seek 1ml @13:19:00.83 delay 1 second @13:19:01.85 AFV.select :holdingCoil @13:19:01.96 delay 1 second @13:19:02.97 ATV.dial :waste @13:19:04.75 AS.reconfigure ASnorm @13:19:04.81 AS.seek :empty @13:19:55.58 AFV.select :carrierSolution @13:19:55.71 delay 2 seconds @13:19:57.73 AS.reconfigure ASconfig @13:19:57.79 AS.seek 0.5ml @13:20:26.90 delay 1 second @13:20:27.92 AFV.select :holdingCoil @13:20:28.03 Building Slug @13:20:28.04 ATV.dial :ARV @13:20:29.68 delay 2 seconds @13:20:31.70 ARV.dial :air1 @13:20:32.12 delay 2 seconds @13:20:34.19 AS.reconfigure ASslow2 @13:20:34.26 AS.seek 0.506ml @13:20:40.58 delay 2 seconds @13:20:42.60 ARV.dial :MM1 @13:20:43.21 delay 2 seconds @13:20:45.24 Adding MM1 @13:20:45.32 AS.seek 0.524ml @13:21:03.57 delay 25 seconds @13:21:28.59 ARV.dial :PP1 @13:21:28.99 Adding PP1 @13:21:29.01 delay 2 seconds @13:21:31.08 AS.seek 0.53ml @13:21:37.32 delay 10 seconds @13:21:47.35 ARV.dial :air1 @13:21:47.76 adding ARV air @13:21:47.78 delay 2 seconds @13:21:49.86 AS.seek 0.536ml @13:21:56.08 delay 5 seconds @13:22:01.10 ARV.dial :carrier @13:22:01.52 delay 7 seconds @13:22:08.61 AS.seek 0.594ml @13:23:07.05 delay 5 seconds @13:23:12.07 ATV.dial :elutionWater @13:23:13.51 delay 2 seconds @13:23:15.59 AS.seek 0.6ml @13:23:21.79 delay 4 seconds @13:23:25.81 ATV.dial ARV @13:23:27.23 delay 2 seconds @13:23:29.25 ARV.dial :carrier @13:23:29.27 delay 2 seconds @13:23:31.35 AS.seek 0.65ml @13:24:21.82 delay 4 seconds @13:24:25.84 ATV.dial PCR @13:24:26.62 ASV.dial :waste1 @13:24:26.63 ARV.dial :air1 @13:24:27.06 Positioning Slug by 0.324ml @13:24:27.15 AS.seek 0.326ml @13:29:53.22 delay 5 seconds @13:29:58.24 ATV.dial ARV @13:29:59.12 ASV.dial :PCRlock @13:29:59.51 delay 5 seconds @13:30:04.54 Running IPc ProfileIPc thermocycling @13:30:04.55 PCR.program ProfileIPc @13:30:04.71 ProfileIPc should complete in < 1:50:49 @13:30:04.73 PCR.run 2:23:31.25 @14:53:57.61 PCR.off @14:53:58.46 Session Ended @14:54:00.14 PCR for IPc completed -- closed /var/log/esp/IPc18nov2013h.pcr @14:54:00.20 Cleaning PCR @14:54:00.21 AFV.select :carrierSolution @14:54:00.32 delay 2 seconds @14:54:02.34 AS.reconfigure ASconfig @14:54:02.88 AS.seek 0.5ml @14:54:13.17 delay 1 second @14:54:14.19 AFV.select :holdingCoil @14:54:14.29 ASV.dial :waste1 @14:54:14.67 ATV.dial :air3 @14:54:15.93 AS.reconfigure ASslow1 @14:54:15.97 AS.seek 0.51ml @14:54:18.27 delay 2 seconds @14:54:20.29 ATV.dial :bleach @14:54:20.90 AS.seek 0.81ml @14:55:21.51 delay 3 seconds @14:55:24.53 ATV.dial :air3 @14:55:25.08 delay 3 seconds @14:55:28.15 AS.seek 0.835ml @14:55:33.45 delay 3 seconds @14:55:36.47 ATV.dial :bleach @14:55:37.07 AS.seek 0.82ml @14:55:40.36 delay 3 seconds @14:55:43.38 ARV.dial :waste @14:55:44.00 delay 3 seconds @14:55:47.02 ATV.dial :ARV @14:55:47.90 AS.reconfigure ASconfig @14:55:47.94 AS.seek 0.47ml @14:56:08.34 AFV.select :carrierSolution @14:56:08.45 delay 2 seconds @14:56:10.47 AS.seek 1ml @14:56:41.23 delay 1 second @14:56:42.25 AFV.select :holdingCoil @14:56:42.36 delay 1 second @14:56:43.38 AS.reconfigure ASfast @14:56:43.43 AS.seek 0ml @14:57:10.83 AFV.select :carrierSolution @14:57:10.94 delay 2 seconds @14:57:12.96 AS.reconfigure ASconfig @14:57:13.01 AS.seek 1ml @14:58:10.76 delay 1 second @14:58:11.78 AFV.select :holdingCoil @14:58:11.89 delay 1 second @14:58:12.91 AS.reconfigure ASfast @14:58:12.96 AS.seek 0ml @14:58:40.28 AFV.select :carrierSolution @14:58:40.39 delay 2 seconds @14:58:42.41 AS.reconfigure ASconfig @14:58:42.46 AS.seek 1ml @14:59:40.23 delay 1 second @14:59:41.25 AFV.select :holdingCoil @14:59:41.36 delay 1 second @14:59:42.38 AS.reconfigure ASfast @14:59:42.43 AS.seek 0ml @15:00:09.76 ARV.dial between carrier and air1 @15:00:10.26 ATV.dial :air3 @15:00:11.54 AS.reconfigure ASslow1 @15:00:11.58 AS.seek 0.01ml @15:00:13.93 delay 2 seconds @15:00:15.95 ATV.dial :bleach @15:00:16.56 AS.seek 0.81ml @15:02:57.68 delay 3 seconds @15:03:00.70 ATV.dial :air3 @15:03:01.25 delay 3 seconds @15:03:04.32 AS.seek 0.835ml @15:03:09.62 delay 3 seconds @15:03:12.64 ATV.dial :bleach @15:03:13.25 AS.seek 0.82ml @15:03:16.54 delay 3 seconds @15:03:19.56 ATV.dial PCR @15:03:19.95 AS.reconfigure ASconfig @15:03:20.00 AS.seek :empty @15:04:07.45 PCR.program :bleachProfile @15:04:07.50 PCR program should complete in < 2:45 @15:04:07.51 PCR.run 10:00 @15:07:06.29 PCR.off @15:07:06.32 AFV.select :carrierSolution @15:07:06.43 delay 2 seconds @15:07:08.45 AS.seek 1ml @15:08:06.28 delay 1 second @15:08:07.30 AFV.select :holdingCoil @15:08:07.41 delay 1 second @15:08:08.43 AS.reconfigure ASfast @15:08:08.48 AS.seek 0ml @15:08:35.84 delay 3 seconds @15:08:38.86 AFV.select :carrierSolution @15:08:38.97 delay 2 seconds @15:08:40.99 AS.reconfigure ASconfig @15:08:41.04 AS.seek 1ml @15:09:38.79 delay 1 second @15:09:39.81 AFV.select :holdingCoil @15:09:39.92 delay 1 second @15:09:40.94 AS.reconfigure ASfast @15:09:40.99 AS.seek 0ml @15:10:08.34 delay 3 seconds @15:10:11.36 AFV.select :carrierSolution @15:10:11.97 delay 2 seconds @15:10:13.99 AS.reconfigure ASconfig @15:10:14.04 AS.seek 1ml @15:11:11.81 delay 1 second @15:11:12.83 AFV.select :holdingCoil @15:11:12.94 delay 1 second @15:11:13.96 AS.reconfigure ASfast @15:11:14.01 AS.seek 0ml @15:11:41.34 delay 3 seconds @15:11:44.36 AFV.select :carrierSolution @15:11:44.47 delay 2 seconds @15:11:46.49 AS.reconfigure ASconfig @15:11:46.54 AS.seek 1ml @15:12:44.31 delay 1 second @15:12:45.33 AFV.select :holdingCoil @15:12:45.44 delay 1 second @15:12:46.46 AS.reconfigure ASfast @15:12:46.51 AS.seek 0ml @15:13:13.87 delay 3 seconds @15:13:16.89 AFV.select :carrierSolution @15:13:17.00 delay 2 seconds @15:13:19.02 AS.reconfigure ASconfig @15:13:19.08 AS.seek 1ml @15:14:16.86 delay 1 second @15:14:17.88 AFV.select :holdingCoil @15:14:17.99 delay 1 second @15:14:19.01 AS.reconfigure ASnorm @15:14:19.06 AS.seek 0ml @15:15:09.81
Asleep after completing 18tun_postDeploy assays @15:15:09.82 GoodNews.email "{3 => 3, 4 => 12}\nCan@15:05:15, 19.4C, 39% humidity, 14.7psia, 11.952V, 0.827A, 0.827A avg, 9.89W",Subject: "Asleep after completing 18tun_postDeploy assays" @15:15:09.85
Mission runtime was 21:49:57.159943 @11:55:23.59CET21-Nov-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @11:55:25.27 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @11:55:25.53 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @11:55:30.47 Polling Can every 10:00 @11:55:32.22 SPE.coast @11:55:32.27 SPE.configure SPEconfig @11:55:32.35 AS.reconfigure ASconfig @11:55:32.39 AS.hold @11:55:32.43 AS.configure ASconfig @11:55:32.52 SS.reconfigure SSconfig @11:55:32.56 SS.hold @11:55:32.62 SS.configure SSconfig @11:55:32.70 CC.reconfigure CCconfig @11:55:32.74 CC.hold @11:55:32.81 CC.configure CCconfig @11:55:32.89 CS.reconfigure CSconfig @11:55:32.93 CS.hold @11:55:33.00 CS.configure CSconfig @11:55:33.42 PC.reconfigure PCconfig @11:55:33.46 PC.hold @11:55:33.54 PC.configure PCconfig @11:55:33.62 PS.configure PSconfig @11:55:33.73 Forearm.reconfigure LooseForearm @11:55:33.77 Forearm.hold @11:55:33.81 Forearm.configure LooseForearm @11:55:33.90 Elbow.reconfigure LooseElbow @11:55:33.94 Elbow.hold @11:55:33.99 Elbow.configure LooseElbow @11:55:34.08 SC.reconfigure SCloose @11:55:34.12 SC.hold @11:55:34.18 SC.configure SCloose @11:55:34.26 SE.reconfigure SEconfig @11:55:34.30 SE.hold @11:55:34.34 SE.configure SEconfig @11:55:34.38 CH.coast @11:55:34.43 CH.configure CHconfig @11:55:34.46 PH.coast @11:55:34.51 PH.configure PHconfig @11:55:34.54 PCR.off @11:55:34.57 PCR.pidtemp= 50,0.1,0,300,25,25 @11:55:34.59 PCR.caltemp= 2.7073,0.0373,0 @11:55:34.62 All dwarf controllers running firmware version 3.75 @11:55:34.75 Loading /home/esp/esp2/mission/18tun_postDeploy2.rb mission @11:55:34.80 Preparing to sleep until +0:00:15 @11:55:34.82 GoodNews.email "Starting 18tun_postDeploy2 w/pucks in tube 4\n{}\nCan@11:55:30, 19.7C, 39% humidity, 14.8psia, 11.987V, 0.682A, 0.682A avg, 8.18W",Subject: "18tun_postDeploy2 begun" @11:55:34.89 AS.coast @11:55:34.92
Asleep til +0:00:15 @11:55:34.97 Continuing at 11:55 SS.coast @11:55:35.05 CC.coast @11:55:35.08 CS.coast @11:55:35.11 PC.coast @11:55:35.16 Forearm.coast @11:55:35.19 Elbow.coast @11:55:35.22 SC.coast @11:55:35.25 SE.coast @11:55:35.64 Gate.power :core,:OFF @11:55:35.65 Gate.power :raw,:OFF @11:55:35.66 Shutdown @11:55:41.27 Session Begun @11:55:44.21 GoodNews.email "Then, in tube 4: mbaPhase\n/home/esp/esp2/mission/18tun_postDeploy2.rb:103\n{}\nCan@11:55:35, 19.7C, 39% humidity, 14.8psia, 11.987V, 0.676A, 0.676A avg, 8.11W",Subject: "Asleep until 11:55" @11:55:49.78
Powering core bus @11:55:54.76 Gate.power :raw,:ON @11:55:55.40 SPE.coast @11:55:55.47 SPE.configure SPEconfig @11:55:55.58 AS.configure ASconfig @11:55:55.69 SS.configure SSconfig @11:55:55.79 CC.configure CCconfig @11:55:55.89 CS.configure CSconfig @11:55:55.99 PC.configure PCconfig @11:55:56.09 PS.configure PSconfig @11:55:56.22 Forearm.configure LooseForearm @11:55:56.32 Elbow.configure LooseElbow @11:55:56.44 SC.configure SCloose @11:55:56.54 SE.configure SEconfig @11:55:56.59 CH.coast @11:55:56.67 CH.configure CHconfig @11:55:56.71 PH.coast @11:55:56.78 PH.configure PHconfig @11:55:56.84 PCR.off @11:55:56.87 PCR.pidtemp= 50,0.1,0,300,25,25 @11:55:56.91 PCR.caltemp= 2.7073,0.0373,0 @11:55:56.94 All dwarf controllers running firmware version 3.75 @11:55:57.05 Valve.reset @11:55:57.24 PCR.off @11:55:57.27 PCR.pidtemp= 50,0.1,0,300,25,25 @11:55:57.31 PCR.caltemp= 2.7073,0.0373,0 @11:55:57.47 Intake.select :close @11:55:57.50 Exhaust.select :close @11:55:57.55 CTV.dial :air @11:55:57.67 PSV.dial :air @11:55:57.72 CSV.dial :waste1 @11:55:57.86
Forearm.reconfigure LooseForearm @11:55:57.92 Forearm.home.seek :retract @11:55:58.01 SSV.dial :spit,avoiding: :exhaust @11:55:58.39 PS.reconfigure PSconfig @11:55:58.49 PS.home.seek 0ml @11:55:58.64 CS.reconfigure CSfast @11:55:58.67 SS.reconfigure SSconfig @11:55:58.79 CS.home.seek 0ml @11:55:58.83 SS.home.seek 0ml @11:55:58.97 Hand.close @11:55:58.99
Elbow.jog 1500 @11:55:59.67 Elbow.reconfigure LooseElbow @11:55:59.73 Elbow.home.seek :clear @11:56:03.11 SE.reconfigure SEconfig @11:56:03.17 SE.home.seek :down @11:56:03.85 SC.reconfigure SCsloppy @11:56:03.92 SC.home.seek :initial @11:56:06.08 Forearm.seek :retract @11:56:06.17 Elbow.seek :hammer @11:56:07.34 Forearm.reconfigure TightForearm @11:56:07.41 Forearm.seek 5 + 350 counts @11:56:07.77 PS.coast @11:56:08.47
Forearm.seek :retract @11:56:09.58 PC.reconfigure PCconfig @11:56:09.61
CC.reconfigure CCconfig @11:56:09.70 PC.home.seek :open @11:56:09.74
CC.home.seek :open @11:56:13.07 Phase [mbaPhase] @11:56:13.09 GoodNews.email "At 11:56:13 began in tube 4: mbaPhase\n/home/esp/esp2/mission/18tun_postDeploy2.rb:103\n{}\nCan@11:56:13, 19.7C, 39% humidity, 14.8psia, 11.947V, 0.853A, 0.853A avg, 10.19W",Subject: "Started 18tun_postDeploy2 phase mbaPhase" @11:56:13.14CET21-Nov-18
==================> STARTED MBAsuperphase (phase #1) <=== @11:56:13.24 Starting mfb @11:56:13.25 pcrBAC sampling at most 150ml, wcr at most 150ml @11:56:13.33 Loading sh1 puck from tube 4 into Collection Clamp @11:56:13.45 Hand.open @11:56:13.46 Readying the MFB @11:56:13.52 Homing MFB Syringe @11:56:13.54 AFV.select :holdingCoil @11:56:13.70 ATV.dial :air2 @11:56:14.33
SC.reconfigure SCtight @11:56:14.39 SC.seek 4 @11:56:15.22 AS.reconfigure ASconfig @11:56:15.29 AS.home.seek :home @11:56:18.01
SC.reconfigure SCloose @11:56:18.32 Elbow.reconfigure TightElbow @11:56:18.44 Elbow.seek 4 @11:56:18.92 Forearm.seek 4 + 700 counts @11:56:20.01 SE.reconfigure SEpick @11:56:20.09 SE.seek beamBreak @11:56:20.80 AFV.select :carrierSolution @11:56:20.91 delay 2 seconds @11:56:22.27 BACfans.email "Started mfb in tube 4 at 11:56:13CET21-Nov-18\n{}\nCan@11:56:13, 19.7C, 39% humidity, 14.8psia, 11.947V, 0.853A, 0.853A avg, 10.19W",Subject: "pcrBAC sampling at most 150ml, wcr at most 150ml" @11:56:22.36
SE.configure SEconfig @11:56:22.53 Elbow.seek 4 @11:56:22.86 Forearm.seek 4 + 300 counts @11:56:22.95 AS.seek 1ml @11:56:23.07
Forearm.reconfigure LooseForearm @11:56:23.13 Hand.close @11:56:23.94 SE.seek :down @11:56:24.17
Forearm.seek :retract @11:56:25.16 Elbow.seek CC @11:56:25.79 SE.coast @11:56:26.79
Elbow.reconfigure LooseElbow @11:56:26.90 Forearm.reconfigure TightForearm @11:56:26.96 Forearm.seek CC @11:56:28.21 CC.seek :closed @11:56:32.51 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.38 @11:56:32.52 Hand.open @11:56:33.38 Forearm.reconfigure LooseForearm @11:56:33.44 Forearm.seek :retract @11:56:34.42 SC.coast @11:56:34.47 collectSample 150ml w/bubble point of 55psi @11:56:34.48 Priming 7ml @11:56:34.50 Intake.select :close @11:56:34.52 Exhaust.select :close @11:56:34.55 SS.hold @11:56:34.77 Calibrating pressure and temperature sensors @11:56:34.78 CTV.dial :air @11:56:34.81 SSV.dial :spit,avoiding: :exhaust @11:56:34.85 SS.reconfigure SSfast @11:56:34.92 SS.seek 5ml @11:56:38.05 SSV.dial CBV @11:56:38.07
CBV.dial SSV @11:56:39.60 delay 3.8 seconds @11:56:43.52 SSV.dial :spit @11:56:45.03 SS.seek 0ml @11:56:47.87 SSV.dial CBV @11:56:49.37 CTV.dial :intake @11:56:50.26 SSV.dial CBV @11:56:50.28
CBV.dial SSV @11:56:50.31 Allowing 40s for 7.0ml of remaining 7.0ml @11:56:50.34 Intake.select :open @11:56:52.52 Intake @15.8psia, vacuum from 9.3 to 13.3psi @11:56:52.54 SS.reconfigure SSinhale.with maxSpeed: 150 @11:56:52.61 SS.seek 7ml @11:56:57.72 Stroke completed -- waiting for pressure to equalize ... @11:57:14.10 Intake.select :close @11:57:14.26 SSV.dial :exhaust @11:57:15.13 Exhaust.select :open @11:57:15.26 SS.reconfigure SSexhale @11:57:15.32 SS.seek 0ml @11:57:15.85 delay 1 second @11:57:16.87 AFV.select :holdingCoil @11:57:16.98 delay 1 second @11:57:18.00 ATV.dial :waste @11:57:19.08 AS.reconfigure ASnorm @11:57:19.14 AS.home.seek :empty @11:57:19.51
Exhaust.select :close @11:57:19.63 Sampling 150.0ml @11:57:19.64 Intake.select :close @11:57:19.66 Exhaust.select :close @11:57:19.69 SS.reconfigure SSconfig @11:57:19.76 SS.hold @11:57:19.96 CTV.dial :intake @11:57:19.98 SSV.dial CBV @11:57:20.01
CBV.dial SSV @11:57:21.32 Allowing 130s for 25.0ml of remaining 150.0ml @11:57:21.34 Intake.select :open @11:57:23.52 Intake @15.7psia, vacuum from 9.2 to 13.2psi @11:57:23.54 SS.reconfigure SSinhale.with maxSpeed: 150 @11:57:23.61 SS.seek 25ml @11:57:27.24 Vacuum too high -- waiting up to 40s @11:57:33.53 Intake @15.5psia, vacuum from 9.0 to 13.0psi @11:57:33.55 SS.reconfigure SSinhale.with maxSpeed: 135 @11:57:33.62 SS.seek 25ml @11:57:36.55 Vacuum too high -- waiting up to 40s @11:57:42.85 Intake @15.6psia, vacuum from 9.1 to 13.1psi @11:57:42.87 SS.reconfigure SSinhale.with maxSpeed: 122 @11:57:42.94 SS.seek 25ml @11:57:46.14 Vacuum too high -- waiting up to 40s @11:57:51.98 SS.reconfigure SSinhale.with maxSpeed: 110 @11:57:52.04 SS.seek 25ml @11:57:55.24 Vacuum too high -- waiting up to 40s @11:58:02.00 Intake @15.7psia, vacuum from 9.2 to 13.2psi @11:58:02.02 SS.reconfigure SSinhale.with maxSpeed: 99 @11:58:02.09 SS.seek 25ml @11:58:05.45 Vacuum too high -- waiting up to 40s @11:58:11.75 Intake @15.6psia, vacuum from 9.1 to 13.1psi @11:58:11.77 ATV.dial :waste @11:58:11.80
SS.reconfigure SSinhale.with maxSpeed: 89 @11:58:11.82 ARV.dial between carrier and air1 @11:58:11.94
SS.seek 25ml @11:58:12.01 ASV.dial :waste1 @11:58:15.81
Vacuum too high -- waiting up to 40s @11:58:22.32 Intake @15.7psia, vacuum from 9.2 to 13.2psi @11:58:22.33 SS.reconfigure SSinhale.with maxSpeed: 80 @11:58:22.41 SS.seek 25ml @11:58:25.46 Stroke completed -- waiting for pressure to equalize ... @11:58:32.03 Intake.select :close @11:58:32.17 SSV.dial :exhaust @11:58:33.05 Exhaust.select :open @11:58:33.18 SS.reconfigure SSexhale @11:58:33.24 SS.seek 0ml @11:58:42.94 Exhaust.select :close @11:58:43.06 SSV.dial CBV @11:58:43.08
CBV.dial SSV @11:58:43.99 Allowing 130s for 25.0ml of remaining 125.0ml @11:58:44.00 Intake.select :open @11:58:46.19 Intake @15.8psia, vacuum from 9.3 to 13.3psi @11:58:46.20 SS.reconfigure SSinhale.with maxSpeed: 80 @11:58:46.28 SS.seek 25ml @11:58:50.33 Vacuum too high -- waiting up to 40s @11:58:54.76 Intake @15.5psia, vacuum from 9.0 to 13.0psi @11:58:54.78 SS.reconfigure SSinhale.with maxSpeed: 72 @11:58:54.85 SS.seek 25ml @11:58:58.05 Vacuum too high -- waiting up to 40s @11:59:02.71 Intake @15.6psia, vacuum from 9.1 to 13.1psi @11:59:02.72 SS.reconfigure SSinhale.with maxSpeed: 65 @11:59:02.80 SS.seek 25ml @11:59:07.42 Vacuum too high -- waiting up to 40s @11:59:12.05 Intake @15.6psia, vacuum from 9.1 to 13.1psi @11:59:12.07 SS.reconfigure SSinhale.with maxSpeed: 59 @11:59:12.15 SS.seek 25ml @11:59:17.52 Vacuum too high -- waiting up to 40s @11:59:22.20 Intake @15.6psia, vacuum from 9.1 to 13.1psi @11:59:22.22 SS.reconfigure SSinhale.with maxSpeed: 53 @11:59:22.29 SS.seek 25ml @11:59:28.64 Vacuum too high -- waiting up to 40s @11:59:33.98 Intake @15.6psia, vacuum from 9.1 to 13.1psi @11:59:33.99 SS.reconfigure SSinhale.with maxSpeed: 48 @11:59:34.07 SS.seek 25ml @11:59:43.14 Vacuum too high -- waiting up to 40s @11:59:48.53 Intake @15.6psia, vacuum from 9.1 to 13.1psi @11:59:48.54 SS.reconfigure SSinhale.with maxSpeed: 43 @11:59:48.62 SS.seek 25ml @11:59:50.02 Stroke completed -- waiting for pressure to equalize ... @11:59:52.47 Intake.select :close @11:59:52.63 SSV.dial :exhaust @11:59:53.51 Exhaust.select :open @11:59:53.64 SS.reconfigure SSexhale @11:59:53.70 SS.seek 0ml @12:00:03.30 Exhaust.select :close @12:00:03.42 SSV.dial CBV @12:00:03.44
CBV.dial SSV @12:00:04.33 Allowing 130s for 25.0ml of remaining 100.0ml @12:00:04.34 Intake.select :open @12:00:06.53 Intake @15.7psia, vacuum from 9.2 to 13.2psi @12:00:06.54 SS.reconfigure SSinhale.with maxSpeed: 43 @12:00:06.61 SS.seek 25ml @12:00:16.52 Vacuum too high -- waiting up to 40s @12:00:20.26 Intake @15.5psia, vacuum from 9.0 to 13.0psi @12:00:20.27 SS.reconfigure SSinhale.with maxSpeed: 39 @12:00:20.35 SS.seek 25ml @12:00:32.83 Vacuum too high -- waiting up to 40s @12:00:36.78 Intake @15.5psia, vacuum from 9.0 to 13.0psi @12:00:36.80 SS.reconfigure SSinhale.with maxSpeed: 35 @12:00:36.87 SS.seek 25ml @12:01:02.68 Stroke completed -- waiting for pressure to equalize ... @12:01:08.81 Intake.select :close @12:01:08.95 SSV.dial :exhaust @12:01:09.82 Exhaust.select :open @12:01:09.95 SS.reconfigure SSexhale @12:01:10.01 SS.seek 0ml @12:01:19.63 Exhaust.select :close @12:01:19.75 SSV.dial CBV @12:01:19.77
CBV.dial SSV @12:01:20.65 Allowing 130s for 25.0ml of remaining 75.0ml @12:01:20.66 Intake.select :open @12:01:22.85 Intake @15.8psia, vacuum from 9.3 to 13.3psi @12:01:22.86 SS.reconfigure SSinhale.with maxSpeed: 35 @12:01:22.94 SS.seek 25ml @12:01:57.98 Vacuum too high -- waiting up to 40s @12:02:02.18 Intake @15.4psia, vacuum from 8.9 to 12.9psi @12:02:02.20 SS.reconfigure SSinhale.with maxSpeed: 32 @12:02:02.27 SS.seek 25ml @12:02:19.05 Stroke completed -- waiting for pressure to equalize ... @12:02:23.32 Intake.select :close @12:02:23.48 SSV.dial :exhaust @12:02:24.36 Exhaust.select :open @12:02:24.91 SS.reconfigure SSexhale @12:02:24.98 SS.seek 0ml @12:02:34.21 Exhaust.select :close @12:02:34.33 SSV.dial CBV @12:02:34.35
CBV.dial SSV @12:02:35.24 Allowing 130s for 25.0ml of remaining 50.0ml @12:02:35.25 Intake.select :open @12:02:37.44 Intake @15.7psia, vacuum from 9.2 to 13.2psi @12:02:37.45 SS.reconfigure SSinhale.with maxSpeed: 32 @12:02:37.53 SS.seek 25ml @12:03:33.18 Stroke completed -- waiting for pressure to equalize ... @12:03:38.66 Intake.select :close @12:03:38.80 SSV.dial :exhaust @12:03:39.68 Exhaust.select :open @12:03:39.80 SS.reconfigure SSexhale @12:03:39.86 SS.seek 0ml @12:03:49.39 Exhaust.select :close @12:03:49.51 SSV.dial CBV @12:03:49.53
CBV.dial SSV @12:03:50.41 Allowing 130s for 25.0ml of remaining 25.0ml @12:03:50.42 Intake.select :open @12:03:52.61 Intake @15.7psia, vacuum from 9.2 to 13.2psi @12:03:52.62 SS.reconfigure SSinhale.with maxSpeed: 32 @12:03:52.70 SS.seek 25ml @12:04:48.24 Stroke completed -- waiting for pressure to equalize ... @12:04:52.52 Intake.select :close @12:04:52.68 SSV.dial :exhaust @12:04:53.55 Exhaust.select :open @12:04:53.68 SS.reconfigure SSexhale @12:04:53.74 SS.seek 0ml @12:05:03.38 Exhaust.select :close @12:05:03.50 Sampled 150.0ml @12:05:03.51 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @12:05:03.53 Intake.select :close @12:05:03.61 CSV.dial :waste @12:05:03.69 CS.seek 0ml @12:05:03.78 SSV.dial CBV,avoiding: :exhaust @12:05:03.80
CBV.dial SSV @12:05:03.89 CSV.dial :air @12:05:04.75 CS.seek 10ml @12:05:05.97
CTV.dial :air @12:05:06.84 delay 15 seconds @12:05:21.86 SSV.dial :spit,avoiding: :exhaust @12:05:23.38 CTV.dial CSV,avoiding: :intake @12:05:23.42
SS.seek 0ml @12:05:23.56 SSV.dial CBV,avoiding: :exhaust @12:05:32.00 CBV.dial between SSV and waste2 @12:05:32.43 CTV.dial CSV,avoiding: :air @12:05:32.46
CSV.dial CTV @12:05:33.00 CS.reconfigure CSpressurize @12:05:33.06 CS.seek 0ml @12:05:59.32 CSV.dial :air @12:05:59.87 CS.reconfigure CSfast @12:05:59.94 CS.seek 10ml @12:06:26.62 CSV.dial between air and CTV @12:06:26.99 CS.seek 2.524ml @12:06:47.10 CTV.dial CSV,avoiding: :air @12:06:47.13
CSV.dial CTV @12:06:47.48 CS.reconfigure CSpressurize @12:06:47.55 CS.seek 0ml @12:06:51.74 BubblePoint: Intake @70.3psia, Outlet @14.9psia CBV.dial SSV @12:06:52.13 SS.reconfigure SSconfig @12:06:52.19 SS.seek 10ml @12:06:58.33 delay 15 seconds @12:07:13.45 Evacuated: Intake @35.2psia, Outlet @14.1psia @12:07:13.46 SSV.dial :spit,avoiding: :exhaust @12:07:14.97 SS.reconfigure SSexhale @12:07:15.04 SS.seek 0ml @12:07:20.18 SS.reconfigure SSfast @12:07:20.24 SS.seek 5ml @12:07:23.10 SSV.dial CBV,avoiding: :exhaust @12:07:23.13
CBV.dial SSV @12:07:24.67 CTV.dial :air,avoiding: :intake @12:07:26.21 CSV.dial CTV @12:07:26.26
SS.seek 10ml @12:07:27.25 CSV.dial :air @12:07:27.77 CS.reconfigure CSfast @12:07:27.84 CS.seek 0ml @12:07:29.05
SSV.dial :spit,avoiding: :exhaust @12:07:30.57 SS.reconfigure SSexhale @12:07:30.64 SS.seek 0ml @12:07:32.26 CS.coast @12:07:36.02
SS.reconfigure SSfast @12:07:36.11 SS.seek 5ml @12:07:38.96 SSV.dial CBV,avoiding: :exhaust @12:07:38.99
CBV.dial SSV @12:07:41.53 SSV.dial :spit,avoiding: :exhaust @12:07:43.05 SS.reconfigure SSconfig @12:07:43.12 SS.seek 0ml @12:07:46.44 SS.coast @12:07:46.49 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @12:07:46.50 Intake.select :close @12:07:46.60 CSV.dial :waste @12:07:46.72 SSV.dial CBV,avoiding: :exhaust @12:07:46.75
CBV.dial SSV @12:07:47.50 CS.seek 0ml @12:07:47.57 CSV.dial :air @12:07:48.27
CTV.dial :air @12:07:48.28 delay 15 seconds @12:07:48.54 CS.seek 10ml @12:08:03.31
SSV.dial :spit,avoiding: :exhaust @12:08:04.82 CTV.dial CSV,avoiding: :intake @12:08:04.86
SS.reconfigure SSexhale @12:08:04.96 SS.seek 0ml @12:08:05.24 SSV.dial CBV,avoiding: :exhaust @12:08:15.31 CBV.dial between SSV and waste2 @12:08:15.76 CSV.dial between air and CTV @12:08:16.13 CS.seek 8.382ml @12:08:21.26 CTV.dial CSV,avoiding: :air @12:08:21.29
CSV.dial CTV @12:08:21.64 CS.reconfigure CSpressurize @12:08:21.71 CS.seek 0ml @12:08:43.73 CSV.dial :air @12:08:44.26 CS.reconfigure CSfast @12:08:44.33 CS.seek 10ml @12:09:10.87 CSV.dial between air and CTV @12:09:11.25 CS.seek 3.139ml @12:09:29.82 CTV.dial CSV,avoiding: :air @12:09:29.85
CSV.dial CTV @12:09:30.18 CS.reconfigure CSpressurize @12:09:30.25 CS.seek 0ml @12:09:38.38 BubblePoint: Intake @69.9psia, Outlet @14.5psia @12:09:38.39 CBV.dial SSV @12:09:38.78 SS.reconfigure SSconfig @12:09:38.84 SS.seek 10ml @12:09:44.17 delay 15 seconds @12:09:59.29 Evacuated: Intake @37.7psia, Outlet @17.3psia @12:09:59.30 SSV.dial :spit,avoiding: :exhaust @12:10:00.82 SS.reconfigure SSexhale @12:10:00.89 SS.seek 0ml @12:10:05.90 SS.reconfigure SSfast @12:10:05.96 SS.seek 5ml @12:10:08.81 SSV.dial CBV,avoiding: :exhaust @12:10:08.83
CBV.dial SSV @12:10:10.36 CTV.dial :air,avoiding: :intake @12:10:11.92 CSV.dial CTV @12:10:11.96
SS.seek 10ml @12:10:12.95 CSV.dial :air @12:10:13.48 CS.reconfigure CSfast @12:10:13.55 CS.seek 0ml @12:10:14.78
SSV.dial :spit,avoiding: :exhaust @12:10:15.20 CS.coast @12:10:16.32
SS.reconfigure SSexhale @12:10:16.38 SS.seek 0ml @12:10:21.41 SS.reconfigure SSfast @12:10:21.47 SS.seek 5ml @12:10:24.25 SSV.dial CBV,avoiding: :exhaust @12:10:24.28
CBV.dial SSV @12:10:26.83 SSV.dial :spit,avoiding: :exhaust @12:10:28.36 SS.reconfigure SSconfig @12:10:28.43 SS.seek 0ml @12:10:31.69 SS.coast @12:10:31.74 Lysing: Adding 1.5ml lysis @12:10:31.75 CBV.dial between CSV and waste1,avoiding: :DBV @12:10:33.48 CTV.dial CSV @12:10:35.01 CSV.dial CSR @12:10:35.92 Pulling 1ml of CSR.lysis @12:10:35.93 CSR.select :lysis @12:10:36.07 CS.reconfigure CSconfig @12:10:36.13 CS.seek 1ml @12:10:39.67 delay 5 seconds @12:10:43.28 Session Begun @12:10:44.69
CSR.select :air @12:10:44.88 CS.seek 6ml @12:10:58.61 delay 2 seconds @12:11:00.63 CSV.dial :waste1 @12:11:02.28 CS.reconfigure CSfast @12:11:02.35 CS.seek :empty @12:11:18.51 CSV.dial CSR @12:11:20.09 Pulling 1.5ml of CSR.lysis @12:11:20.10 CSR.select :lysis @12:11:20.26 CS.reconfigure CSconfig @12:11:20.32 CS.seek 1.5ml @12:11:25.20 delay 5 seconds @12:11:30.22 CSR.select :air @12:11:30.41 CS.seek 4.5ml @12:11:39.14 delay 2 seconds @12:11:41.16 CSV.dial CTV @12:11:41.69 CS.seek 1.275ml @12:11:51.05 Heating sh1 puck from tube 4 in Collection Clamp to 85C for 5:00 @12:11:51.07 CH.seek 85C,8:00 @12:15:15.08 CH.hold 5:00,85C @12:20:15.24 Collection Puck Heater at 85C after lysing @12:20:15.26 CS.seek 0ml @12:20:19.89 CTV.dial between spare and air,avoiding: :intake @12:20:21.11 CSV.dial :air @12:20:21.65 CS.seek 5ml @12:20:35.36 CSV.dial CTV @12:20:35.89 delay 2 seconds @12:20:37.91 CSV.dial :air @12:20:38.44 delay 2 seconds @12:20:40.46 CSV.dial CTV @12:20:41.02 delay 2 seconds @12:20:43.04 CSV.dial :air @12:20:43.60 delay 2 seconds @12:20:45.63 CS.seek 0ml @12:20:59.39 Recovering Lysate into Collection syringe @12:20:59.40 CSV.dial CBV @12:21:00.63 CBV.dial CSV @12:21:01.08 CS.seek 5ml @12:21:14.80 delay 5 seconds @12:21:19.82 CTV.dial :air @12:21:20.41 CS.seek 7ml @12:21:26.55 delay 5 seconds @12:21:31.57 CSV.dial :air2 @12:21:32.09 CS.seek 10ml @12:21:40.83 CSV.dial between air and CTV,avoiding: CBV @12:21:42.28 Lysing Complete @12:21:42.38 Storing sh1 puck in Collection Clamp to tube 3 @12:21:42.56 SC.reconfigure SCtight @12:21:42.63 SC.seek 3 @12:21:44.74 SC.reconfigure SCloose @12:21:44.96 Elbow.reconfigure TightElbow @12:21:45.03 Elbow.seek 3 @12:21:47.06 Forearm.reconfigure TightForearm @12:21:47.13 Forearm.seek 3 + 700 counts @12:21:48.20 SE.reconfigure SEpick @12:21:48.30 SE.seek beamBreak @12:21:51.29 SE.configure SEconfig @12:21:51.36 SE.seek :place @12:21:51.89 Forearm.reconfigure LooseForearm @12:21:51.97 Forearm.seek :retract @12:21:53.30 Elbow.seek CC @12:21:55.09 Elbow.reconfigure LooseElbow @12:21:55.20 Forearm.seek CC @12:21:56.17 Hand.close @12:21:56.96 CC.seek :open @12:22:01.46 Forearm.seek :retract @12:22:02.49 Elbow.reconfigure TightElbow @12:22:02.57 Elbow.seek 3 @12:22:04.55 Elbow.reconfigure LimpElbow @12:22:04.63 Forearm.seek 3 @12:22:05.70 Elbow.hold @12:22:05.79 Elbow.reconfigure TightElbow @12:22:05.85 Hand.open @12:22:06.69 Forearm.force -20 @12:22:06.76 Forearm.seek 3 + 345 counts @12:22:07.09 Forearm.reconfigure TightForearm @12:22:07.16 Forearm.seek 3 + 700 counts @12:22:07.49 SE.seek :down @12:22:08.63
Forearm.reconfigure LooseForearm @12:22:08.70 Forearm.seek :retract @12:22:10.37 SE.coast @12:22:10.44
SC.coast @12:22:10.49 Tranferring lysate to PRV @12:22:10.50 CSV.dial :air @12:22:10.90 CS.seek 10ml @12:22:11.21 CSV.dial between air and CTV @12:22:11.58 AIV.dial :bypass @12:22:11.80 Beginning samplePassC @12:22:11.81 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:22:11.93 Hand.open @12:22:12.31 Elbow.seek FlushPuck::Garage @12:22:14.24 Elbow.reconfigure LooseElbow @12:22:14.37 Forearm.seek :garage @12:22:15.39 Hand.close @12:22:16.31 Forearm.seek :retract @12:22:17.38 Elbow.reconfigure TightElbow @12:22:17.46 Elbow.seek CC @12:22:20.38 Elbow.reconfigure LooseElbow @12:22:20.51 Forearm.reconfigure TightForearm @12:22:20.58 Forearm.seek CC @12:22:21.84 CC.seek :closed @12:22:26.64 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.36 @12:22:26.76 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[5C:27->20] @12:22:26.78 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[5C:27->20] @12:22:26.80 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[5C:27->20] @12:22:26.82 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[5C:27->20] @12:22:26.85 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[5C:27->20] @12:22:26.87 Hand.open @12:22:27.76 Forearm.reconfigure LooseForearm @12:22:27.84 Forearm.seek :retract @12:22:28.83 AWV.select :air @12:22:28.84 AIV.dial :bypass @12:22:28.88 PSV.dial PSR @12:22:30.46 Pulling 1.5ml of PSR.flush @12:22:30.47 PSR.select :flush @12:22:30.64 PS.seek 1.5ml @12:22:47.00 delay 5 seconds @12:22:52.02 PSV.dial :waste1 @12:22:52.56 PS.seek 0ml @12:23:09.03 PSV.dial PSR @12:23:09.58 PSR.select :air @12:23:09.72 PS.seek 2.5ml @12:23:36.38 PRV.dial CBV @12:23:36.41
CBV.dial PRV @12:23:37.36 PSV.dial :mixloop @12:23:38.53 CTV.dial :air,avoiding: :intake @12:23:38.54 PTV.dial :PRVmixing @12:23:38.67 PS.seek 0ml @12:24:05.57 PSV.dial :air @12:24:06.11 PS.seek 2.5ml @12:24:32.60 PSV.dial :mixloop @12:24:33.14 PS.seek 0ml @12:24:59.72 PSV.dial :air @12:25:00.26 PS.seek 2.5ml @12:25:26.60 PSV.dial :mixloop @12:25:27.14 PS.seek 0ml @12:25:53.94 Garaging FlushPuck in Collection Clamp @12:25:54.04 Elbow.reconfigure TightElbow @12:25:54.11 Elbow.seek CC @12:25:54.35 Elbow.reconfigure LooseElbow @12:25:54.47 Forearm.seek CC @12:25:55.46 Hand.close @12:25:56.21 CC.seek :open @12:26:00.57 Forearm.seek :retract @12:26:01.55 Elbow.reconfigure TightElbow @12:26:01.63 Elbow.seek :garage @12:26:04.73 Elbow.reconfigure LooseElbow @12:26:04.85 Forearm.reconfigure TightForearm @12:26:04.92 Forearm.seek :garage @12:26:06.10 Hand.open @12:26:06.89 Forearm.reconfigure LooseForearm @12:26:06.96 Forearm.seek :retract @12:26:08.05 Connecting PS to CS to transfer sample @12:26:08.06 CBV.dial AIV @12:26:08.10 AIV.dial :bypass @12:26:08.13 PSV.dial :mixloop @12:26:08.16 PTV.dial :PRVmixing @12:26:08.19 PRV.dial AIV @12:26:08.22 CTV.dial :air,avoiding: :intake @12:26:08.26 Loading lyfil puck from tube 4 into Collection Clamp @12:26:08.52 SC.reconfigure SCtight @12:26:08.59 SC.seek 4 @12:26:10.69 SC.reconfigure SCloose @12:26:10.91 Elbow.reconfigure TightElbow @12:26:10.98 Elbow.seek 4 @12:26:13.47 Forearm.reconfigure TightForearm @12:26:13.54 Forearm.seek 4 + 700 counts @12:26:14.61 SE.reconfigure SEpick @12:26:14.71 SE.seek beamBreak @12:26:17.02 SE.configure SEconfig @12:26:17.19 Elbow.seek 4 @12:26:17.38 Forearm.seek 4 + 300 counts @12:26:17.65 Forearm.reconfigure LooseForearm @12:26:17.72 Hand.close @12:26:18.47 SE.seek :down @12:26:18.68
Forearm.seek :retract @12:26:19.69 Elbow.seek CC @12:26:20.43 SE.coast @12:26:21.35
Elbow.reconfigure LooseElbow @12:26:21.47 Forearm.reconfigure TightForearm @12:26:21.53 Forearm.seek CC @12:26:22.79 CC.seek :closed @12:26:27.07 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.45 @12:26:27.09 Hand.open @12:26:27.89 Forearm.reconfigure LooseForearm @12:26:27.96 Forearm.seek :retract @12:26:28.97 SC.coast @12:26:29.02 CTV.dial CSV,avoiding: :intake @12:26:30.57 CSV.dial CTV @12:26:30.94 CS.seek 5ml @12:26:44.68 delay 1:20 @12:28:04.72 CS.seek 2.5ml @12:28:12.15 delay 40 seconds @12:28:52.18 CS.seek 0ml @12:28:59.52 delay 1:00 @12:29:59.54 PSV.dial :air @12:30:00.05 PS.seek 2.5ml @12:30:26.47 PSV.dial :mixloop @12:30:26.97 delay 3 seconds @12:30:29.99 PSV.dial :air @12:30:30.49 delay 2 seconds @12:30:32.51 PSV.dial :mixloop @12:30:33.02 delay 3 seconds @12:30:36.04 PSV.dial :waste1 @12:30:37.56 PS.seek :empty @12:31:04.29 CBV.dial :waste1 @12:31:04.82 delay 5 seconds @12:31:09.84 CBV.dial AIV @12:31:10.37 delay 10 seconds @12:31:20.40 CBV.dial :waste1 @12:31:20.93 delay 10 seconds @12:31:30.96 CBV.dial AIV @12:31:31.49 delay 10 seconds @12:31:41.52 CTV.dial CSV,avoiding: :intake @12:31:41.53 CBV.dial between AIV and waste1 @12:31:41.95 CSV.dial :air @12:31:42.49 CS.seek 10ml @12:32:09.09 CSV.dial CTV @12:32:09.60 CS.seek 0ml @12:32:36.34 CBV.dial :waste1 @12:32:36.68 delay 10 seconds @12:32:46.71 CTV.dial :air,avoiding: :intake @12:32:48.32 Storing lyfil puck in Collection Clamp to tube 3 @12:32:48.97 SC.reconfigure SCtight @12:32:49.04 SC.seek 3 @12:32:51.17 SC.reconfigure SCloose @12:32:51.39 Elbow.reconfigure TightElbow @12:32:51.47 Elbow.seek 3 @12:32:53.48 Forearm.reconfigure TightForearm @12:32:53.56 Forearm.seek 3 + 700 counts @12:32:54.56 SE.reconfigure SEpick @12:32:54.66 SE.seek beamBreak @12:32:57.89 SE.configure SEconfig @12:32:58.06 Elbow.reconfigure LimpElbow @12:32:58.13 Forearm.reconfigure LooseForearm @12:32:58.20 Forearm.seek 3 @12:32:58.51 Elbow.hold @12:32:58.60 Elbow.reconfigure TightElbow @12:32:58.73 SE.seek 0.07 Pucks below Top Plate @12:32:59.17 Forearm.force -20 @12:32:59.23 Forearm.seek 3 + 350 counts @12:32:59.53 Forearm.reconfigure TightForearm @12:32:59.60 Forearm.seek 3 + 700 counts @12:32:59.85 SE.seek 1.07 Pucks below Top Plate @12:33:00.50 Forearm.reconfigure LooseForearm @12:33:00.58 Forearm.seek :retract @12:33:01.55 Elbow.seek CC @12:33:03.24 Elbow.reconfigure LooseElbow @12:33:03.36 Forearm.seek CC @12:33:04.32 Hand.close @12:33:05.09 CC.seek :open @12:33:09.59 Forearm.seek :retract @12:33:10.61 Elbow.reconfigure TightElbow @12:33:10.69 Elbow.seek 3 @12:33:12.66 Elbow.reconfigure LimpElbow @12:33:12.74 Forearm.seek 3 @12:33:13.76 Elbow.hold @12:33:13.85 Elbow.reconfigure TightElbow @12:33:13.92 Hand.open @12:33:14.84 Forearm.force -20 @12:33:14.90 Forearm.seek 3 + 350 counts @12:33:15.21 Forearm.reconfigure TightForearm @12:33:15.28 Forearm.seek 3 + 700 counts @12:33:15.59 SE.seek :down @12:33:16.73
Forearm.reconfigure LooseForearm @12:33:16.80 Forearm.seek :retract @12:33:18.29 SE.coast @12:33:18.35
SC.coast @12:33:18.41 PSV.dial :air @12:33:19.63 PS.seek 0.499ml @12:33:25.60 delay 3 seconds @12:33:28.62 PSV.dial :mixloop @12:33:29.12 PRV.dial :air @12:33:29.65 delay 3 seconds @12:33:32.67 PRV.dial CBV @12:33:33.65 CBV.dial AIV @12:33:34.20 PRV.dial between CBV and air @12:33:34.57 PRV.dial :air @12:33:34.97 PS.reconfigure PSslow @12:33:35.04 PS.seek 0.599ml @12:33:37.57 PRV.dial :waste2 @12:33:38.41 PS.reconfigure PSconfig @12:33:38.48 PS.seek :empty @12:33:45.44 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:33:45.53 Hand.open @12:33:45.95 Elbow.seek FlushPuck::Garage @12:33:47.87 Elbow.reconfigure LooseElbow @12:33:47.98 Forearm.seek :garage @12:33:49.05 Hand.close @12:33:49.84 Forearm.seek :retract @12:33:50.92 Elbow.reconfigure TightElbow @12:33:50.99 Elbow.seek CC @12:33:53.78 Elbow.reconfigure LooseElbow @12:33:53.91 Forearm.reconfigure TightForearm @12:33:53.97 Forearm.seek CC @12:33:55.28 CC.seek :closed @12:33:59.55 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.22 @12:33:59.65 Hand.open @12:34:00.53 Forearm.reconfigure LooseForearm @12:34:00.60 Forearm.seek :retract @12:34:01.61 Clearing PML for modified lysate @12:34:01.62 CTV.dial :air,avoiding: :intake @12:34:01.66 PRV.dial :waste2 @12:34:01.68 PTV.dial :PRV,avoiding: :puck @12:34:02.55 delay 2 seconds @12:34:04.57 PTV.dial :PRVmixing,avoiding: :puck @12:34:05.41 delay 2 seconds @12:34:07.43 PSV.dial :PML @12:34:07.45 delay 2 seconds @12:34:09.50 PRV.dial :air @12:34:10.35 delay 2 seconds @12:34:12.42 PS.seek 1.499ml @12:34:28.76 delay 5 seconds @12:34:33.79 PS.seek 2.5ml @12:34:45.07 PSV.dial :air @12:34:45.61 PS.seek 0ml @12:35:12.51 mixing lysate with speDil @12:35:12.52 PSV.dial :PML @12:35:13.07 CBV.dial :PRV @12:35:13.08 PRV.dial :air @12:35:13.12 delay 2 seconds @12:35:15.15 PRV.dial :speDil @12:35:15.68 delay 2 seconds @12:35:17.77 PS.reconfigure PSslow1 @12:35:17.84 PS.seek 0.225ml @12:35:41.69 delay 5 seconds @12:35:46.71 PRV.dial CBV @12:35:47.85 delay 2 seconds @12:35:49.93 PS.seek 0.475ml @12:36:15.63 delay 5 seconds @12:36:20.65 PRV.dial :air @12:36:21.19 delay 2 seconds @12:36:23.67 PS.seek 0.965ml @12:37:14.13 delay 10 seconds @12:37:24.16 PRV.dial CBV @12:37:24.71 CTV.dial :air,avoiding: :intake @12:37:24.72 PSV.dial :air @12:37:25.27 PS.reconfigure PSconfig @12:37:25.35 PS.seek 2.5ml @12:37:41.98 delay 3 seconds @12:37:45.00 PSV.dial PTV @12:37:45.98 PTV.dial :PRV,avoiding: :puck @12:37:46.86 PS.seek 0ml @12:38:13.38 PSV.dial :air @12:38:14.24 PS.seek 2.5ml @12:38:40.88 delay 3 seconds @12:38:43.90 PSV.dial PTV @12:38:44.76 PTV.dial :PRV,avoiding: :puck @12:38:44.78 PS.seek 0ml @12:39:11.51 Pushing modified lysate into AIV Injection Loop @12:39:11.52 CBV.dial AIV @12:39:11.56 AIV.dial :bypass @12:39:11.59 PRV.dial CBV @12:39:11.63 PSV.dial :air @12:39:12.50 PS.seek 2.5ml @12:39:39.13 delay 3 seconds @12:39:42.15 PSV.dial :PML @12:39:42.67 PTV.dial :PRVmixing,avoiding: :puck @12:39:43.58 PS.reconfigure PSslow1 @12:39:43.66 PS.seek 1.74ml @12:41:01.10 delay 5 seconds @12:41:06.13 AIV.dial :sample @12:41:06.78 AWV.select :waste @12:41:06.96 PS.reconfigure PSslow2 @12:41:07.04 PS.seek 1.22ml @12:42:54.73 delay 15 seconds @12:43:09.75 AIV.dial :bypass @12:43:10.41 PS.reconfigure PSconfig @12:43:10.49 PS.seek 0ml @12:43:23.98 PSV.dial :air @12:43:24.52 PS.seek 2.5ml @12:43:50.98 PSV.dial :PML @12:43:51.52 PS.seek 0ml @12:44:08.38 Session Begun @12:44:12.67 -> showlog100 @12:44:12.69 NameError in bkha -- undefined local variable or method `showlog100' for # @12:44:18.17
AIV.dial :coilToMFB @12:44:18.18 AWV.select :air @12:44:18.41 Extracting Nucleic Acid from AIV @12:44:18.43
Beginning samplePassD @12:44:18.50 SPE.hold 2:46:40,55C @12:44:18.63 ASV.dial :SPEdelivery @12:44:18.70 -> showlog 100 @12:44:18.82
AWV.select :air @12:44:18.84 AIV.dial :bypass @12:44:18.93 PSV.dial PSR @12:44:19.08 delay 20 seconds @12:44:20.29
Pulling 1.5ml of PSR.killMFB @12:44:20.30 PSR.select :killMFB @12:44:20.49 PS.seek 1.5ml @12:44:35.96 -> showlog 1000 @12:44:37.60
delay 5 seconds @12:44:39.10 primeLysateINT @12:44:39.11 AWV.select :air @12:44:39.16 AIV.dial :coilToMFB @12:44:39.19 ATV.dial AIV @12:44:41.12 AS.reconfigure ASslow1 @12:44:41.20 AS.seek 0.299ml @12:44:42.62
PSV.dial :waste1 @12:44:43.19 PS.seek 0ml @12:45:00.23 PSV.dial PSR @12:45:00.81 PSR.select :air @12:45:00.97 PS.seek 2.5ml @12:45:29.07 PRV.dial CBV @12:45:29.10
CBV.dial PRV @12:45:29.15 PSV.dial :mixloop @12:45:30.40 CTV.dial :air,avoiding: :intake @12:45:30.41 PTV.dial :PRVmixing @12:45:30.93 PS.seek 0ml @12:45:41.86 delay 10 seconds @12:45:51.89 ATV.dial :air2 @12:45:52.81 AS.seek 0.329ml @12:45:59.07
PSV.dial PSR @12:45:59.18 ATV.dial :waste2 @12:45:59.80 AS.reconfigure ASnorm @12:45:59.89 AS.seek :empty @12:46:00.40
Pulling 1.5ml of PSR.flush @12:46:00.41 PSR.select :flush @12:46:00.61 PS.seek 1.5ml @12:46:09.03 -> exit @12:46:09.05 Session Ended @12:46:16.93 Lysate primed! @12:46:16.94 AFV.select :holdingCoil @12:46:16.97 ATV.dial :air2 @12:46:17.64 AS.reconfigure ASslow1 @12:46:17.71 AS.seek 0.069ml @12:46:17.82
delay 5 seconds @12:46:22.84 PSV.dial :waste1 @12:46:23.40 PS.seek 0ml @12:46:32.15 delay 3 seconds @12:46:35.17 ATV.dial :AIV @12:46:36.08 AS.seek 0.469ml @12:46:40.45
PSV.dial PSR @12:46:41.01 PSR.select :air @12:46:41.16 PS.seek 2.5ml @12:47:09.23 PRV.dial CBV @12:47:09.27
CBV.dial PRV @12:47:09.29 PSV.dial :mixloop @12:47:10.54 CTV.dial :air,avoiding: :intake @12:47:10.55 PTV.dial :PRVmixing @12:47:10.61 PS.seek 0ml @12:47:38.39 PSV.dial PSR @12:47:39.67 Pulling 1.5ml of PSR.flush @12:47:39.68 PSR.select :flush @12:47:39.86 PS.seek 1.5ml @12:47:56.70 delay 5 seconds @12:47:56.85 delay 5 seconds @12:48:01.72
PSV.dial :waste1 @12:48:01.91 Solid Phase Extraction Heater at 48.8C @12:48:01.92 ATV.dial ASV @12:48:02.27
PS.seek 0ml @12:48:02.36 ASV.dial :SPEdelivery @12:48:02.46 AS.seek 0.041ml @12:48:18.92
PSV.dial PSR @12:48:19.46 PSR.select :air @12:48:19.60 PS.seek 2.5ml @12:48:46.73 PRV.dial CBV @12:48:46.77
CBV.dial PRV @12:48:46.79 PSV.dial :mixloop @12:48:48.01 CTV.dial :air,avoiding: :intake @12:48:48.02 PTV.dial :PRVmixing @12:48:48.08 PS.seek 0ml @12:49:15.11 PSV.dial PSR @12:49:16.36 Pulling 1.5ml of PSR.flush @12:49:16.37 PSR.select :flush @12:49:16.54 PS.seek 1.5ml @12:49:28.85 delay 10 seconds @12:49:33.14
delay 5 seconds @12:49:38.16 PSV.dial :waste1 @12:49:38.71 PS.seek 0ml @12:49:38.87 rinseLysateLineINT @12:49:38.88 AFV.select :carrierSolution @12:49:39.02 delay 2 seconds @12:49:41.05 AS.reconfigure ASconfig @12:49:41.12 AS.seek 1ml @12:49:55.23
PSV.dial PSR @12:49:55.78 PSR.select :air @12:49:55.92 PS.seek 2.5ml @12:50:22.82 PRV.dial CBV @12:50:22.85
CBV.dial PRV @12:50:22.88 PSV.dial :mixloop @12:50:24.08 CTV.dial :air,avoiding: :intake @12:50:24.09 PTV.dial :PRVmixing @12:50:24.15 PS.reconfigure PSslow @12:50:24.22 PS.seek 1.2ml @12:50:36.52 delay 1 second @12:50:37.54 AFV.select :holdingCoil @12:50:37.64 delay 1 second @12:50:38.66 ATV.dial AIV @12:50:39.22 AIV.dial :coilToMFB @12:50:39.63 AWV.select :waste @12:50:39.85 AS.reconfigure ASslow0 @12:50:39.91 AS.seek 0ml @12:50:51.04
PSV.dial :waste1 @12:50:52.59 PS.reconfigure PSconfig @12:50:52.66 PS.seek 0ml @12:51:06.14 PSV.dial :air @12:51:07.35 PS.seek 2.5ml @12:51:34.33 PSV.dial :mixloop @12:51:34.84 PS.reconfigure PSslow @12:51:34.92 PS.seek 0ml @12:52:20.82 ATV.dial :air2 @12:52:21.52 AS.reconfigure ASslow1 @12:52:21.59 AS.seek 0.05ml @12:52:26.02
PSV.dial :air @12:52:26.54 PS.reconfigure PSconfig @12:52:26.62 PS.seek 2.5ml @12:52:31.97 ATV.dial :AIV @12:52:32.82 AS.seek 0.01ml @12:52:41.16 ATV.dial :waste @12:52:42.83 AS.seek :empty @12:52:45.30 ethanol wash @12:52:45.31 ATV.dial :air2 @12:52:46.61 AS.reconfigure ASslow2 @12:52:46.68 AS.seek 0.009ml @12:52:53.45
PSV.dial :mixloop @12:52:53.98 PS.reconfigure PSslow @12:52:54.06 PS.seek 0ml @12:52:56.99 delay 3 seconds @12:53:00.01 Pulling 0.4ml of Ethanol Wash @12:53:00.02 ATV.dial :ethanol @12:53:00.89 AS.reconfigure ASslow1 @12:53:00.96 AS.seek 0.409ml @12:53:45.26
Cleaning Collection Syringe @12:53:45.27 CSV.dial CSR @12:53:45.88 Pulling 4ml of CSR.flush CSR.select :flush @12:53:46.05 CS.seek 4ml @12:53:57.45 delay 5 seconds @12:54:02.47 CSR.select :air @12:54:02.62 CS.seek 10ml @12:54:18.85 CSV.dial CTV @12:54:18.88
CTV.dial CSV @12:54:20.51 CBV.dial :waste1 @12:54:21.07 CS.seek 0ml @12:54:21.68 delay 3 seconds @12:54:24.70 ATV.dial :air1 @12:54:25.11 delay 3 seconds @12:54:28.18 AS.seek 0.434ml @12:54:33.49 delay 3 seconds @12:54:36.51 ATV.dial :ethanol @12:54:37.14 AS.seek 0.419ml @12:54:40.41 delay 3 seconds @12:54:43.43 ATV.dial ASV @12:54:44.78 AS.seek 0.092ml @12:54:47.67
CSV.dial CSR @12:54:48.26 Pulling 4ml of CSR.flush @12:54:48.27 CSR.select :flush @12:54:48.44 CS.seek 4ml @12:54:59.63 delay 5 seconds @12:55:04.65 CSR.select :air @12:55:04.81 CS.seek 10ml @12:55:21.13 CSV.dial CTV @12:55:21.16
CTV.dial CSV @12:55:21.73 CBV.dial :waste1 @12:55:21.75 CS.seek 0ml @12:55:48.41 Garaging FlushPuck in Collection Clamp @12:55:48.50 Elbow.reconfigure TightElbow @12:55:48.57 Elbow.seek CC @12:55:48.70 Elbow.reconfigure LooseElbow @12:55:48.82 Forearm.seek CC @12:55:49.79 Hand.close @12:55:50.96 CC.seek :open @12:55:51.06 delay 3 seconds @12:55:54.08 ATV.dial :waste2 @12:55:54.88 AS.reconfigure ASconfig @12:55:54.95 AS.seek :empty @12:55:55.38
Forearm.seek :retract @12:55:56.39 Elbow.reconfigure TightElbow @12:55:56.47 Elbow.seek :garage @12:55:59.60 Elbow.reconfigure LooseElbow @12:55:59.71 Forearm.reconfigure TightForearm @12:55:59.78 Forearm.seek :garage @12:56:00.62 ATV.dial :air2 @12:56:00.96
Hand.open @12:56:01.05 AS.reconfigure ASslow1 @12:56:01.12 AS.seek 1ml @12:56:01.81
Forearm.reconfigure LooseForearm @12:56:01.88 Forearm.seek :retract @12:56:02.95 Whole Cell Archive sampling 150ml @12:56:02.97 Loading wcr puck from tube 4 into Collection Clamp @12:56:03.25 SC.reconfigure SCtight @12:56:03.32 SC.seek 4 @12:56:05.45 SC.reconfigure SCloose @12:56:05.69 Elbow.reconfigure TightElbow @12:56:05.77 Elbow.seek 4 @12:56:07.91 Forearm.reconfigure TightForearm @12:56:07.99 Forearm.seek 4 + 700 counts @12:56:08.99 SE.reconfigure SEpick @12:56:09.10 SE.seek beamBreak @12:56:11.48 SE.configure SEconfig @12:56:11.66 Elbow.seek 4 @12:56:11.86 Forearm.seek 4 + 300 counts @12:56:12.14 Forearm.reconfigure LooseForearm @12:56:12.21 Hand.close @12:56:13.02 SE.seek :down @12:56:13.25 Forearm.seek :retract @12:56:14.21 Elbow.seek CC @12:56:15.07 SE.coast @12:56:15.87 Elbow.reconfigure LooseElbow @12:56:15.99 Forearm.reconfigure TightForearm @12:56:16.06 Forearm.seek CC @12:56:17.34 CC.seek :closed @12:56:21.63 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.39 @12:56:21.64 Hand.open @12:56:22.56 Forearm.reconfigure LooseForearm @12:56:23.11 Forearm.seek :retract @12:56:24.14 SC.coast @12:56:24.20 collectSample 150ml w/bubble point of 55psi @12:56:24.22 Priming 7ml @12:56:24.25 Intake.select :close @12:56:24.28 Exhaust.select :close @12:56:24.32 SS.hold @12:56:24.56 Calibrating pressure sensors @12:56:24.57 CTV.dial :air @12:56:26.15 SSV.dial :spit,avoiding: :exhaust @12:56:26.17 SS.reconfigure SSfast @12:56:26.26 SS.seek 5ml @12:56:28.99 SSV.dial CBV @12:56:29.02 CBV.dial SSV @12:56:30.65 delay 3.8 seconds @12:56:34.57 SSV.dial :spit @12:56:36.11 SS.seek 0ml @12:56:38.87 SSV.dial CBV @12:56:40.41 CTV.dial :intake @12:56:41.29 SSV.dial CBV @12:56:41.32 CBV.dial SSV @12:56:41.35 Allowing 40s for 7.0ml of remaining 7.0ml @12:56:41.39 Intake.select :open @12:56:43.59 Intake @15.9psia, vacuum from 9.4 to 13.4psi @12:56:43.61 SS.reconfigure SSinhale.with maxSpeed: 150 @12:56:43.71 SS.seek 7ml @12:56:49.16 Stroke completed -- waiting for pressure to equalize ... @12:57:07.53 Intake.select :close @12:57:07.70 SSV.dial :exhaust @12:57:08.59 Exhaust.select :open @12:57:08.73 SS.reconfigure SSexhale @12:57:08.80 SS.seek 0ml @12:57:12.91 Exhaust.select :close @12:57:13.05 Sampling 150.0ml @12:57:13.06 Intake.select :close @12:57:13.08 Exhaust.select :close @12:57:13.13 SS.reconfigure SSconfig @12:57:13.21 SS.hold @12:57:13.91 CTV.dial :intake @12:57:13.94 SSV.dial CBV @12:57:13.98 CBV.dial SSV @12:57:14.90 Allowing 130s for 25.0ml of remaining 150.0ml @12:57:14.92 Intake.select :open @12:57:17.13 Intake @15.7psia, vacuum from 9.2 to 13.2psi @12:57:17.15 SS.reconfigure SSinhale.with maxSpeed: 150 @12:57:17.24 SS.seek 25ml @12:57:22.25 Vacuum too high -- waiting up to 40s @12:57:32.55 -> exit @12:57:32.57 Session Ended @12:57:33.70 Intake @15.7psia, vacuum from 9.2 to 13.2psi @12:57:33.72 SS.reconfigure SSinhale.with maxSpeed: 135 @12:57:33.79 SS.seek 25ml @12:57:38.57 Vacuum too high -- waiting up to 40s @12:57:51.09 SS.reconfigure SSinhale.with maxSpeed: 122 @12:57:51.15 SS.seek 25ml @12:57:56.85 Vacuum too high -- waiting up to 40s @12:58:11.02 Intake @15.6psia, vacuum from 9.1 to 13.1psi @12:58:11.04 SS.reconfigure SSinhale.with maxSpeed: 110 @12:58:11.11 SS.seek 25ml @12:58:15.18 Stroke completed -- waiting for pressure to equalize ... @12:58:26.22 Intake.select :close @12:58:26.38 SSV.dial :exhaust @12:58:27.26 Exhaust.select :open @12:58:27.39 SS.reconfigure SSexhale @12:58:27.45 SS.seek 0ml @12:58:36.66 Exhaust.select :close @12:58:36.79 SSV.dial CBV @12:58:36.81 CBV.dial SSV @12:58:37.69 Allowing 130s for 25.0ml of remaining 125.0ml @12:58:37.71 Intake.select :open @12:58:39.90 Intake @15.6psia, vacuum from 9.1 to 13.1psi @12:58:39.92 SS.reconfigure SSinhale.with maxSpeed: 110 @12:58:39.99 SS.seek 25ml @12:58:45.44 Vacuum too high -- waiting up to 40s @12:58:53.61 Intake @15.5psia, vacuum from 9.0 to 13.0psi @12:58:53.63 SS.reconfigure SSinhale.with maxSpeed: 99 @12:58:53.71 SS.seek 25ml @12:58:58.69 Vacuum too high -- waiting up to 40s @12:59:08.21 Intake @15.5psia, vacuum from 9.0 to 13.0psi @12:59:08.23 SS.reconfigure SSinhale.with maxSpeed: 89 @12:59:08.31 SS.seek 25ml @12:59:13.00 Vacuum too high -- waiting up to 40s @12:59:21.41 Intake @15.5psia, vacuum from 9.0 to 13.0psi @12:59:21.43 SS.reconfigure SSinhale.with maxSpeed: 80 @12:59:21.51 SS.seek 25ml @12:59:22.72 delay 5 seconds @12:59:27.63 Vacuum too high -- waiting up to 40s @12:59:27.74 ATV.dial ASV @12:59:28.83 ASV.dial :SPEdelivery @12:59:28.85 AS.seek 0.02ml @12:59:37.22 Intake @15.5psia, vacuum from 9.0 to 13.0psi @12:59:37.24 SS.reconfigure SSinhale.with maxSpeed: 72 @12:59:37.31 SS.seek 25ml @12:59:39.54 Stroke completed -- waiting for pressure to equalize ... @12:59:45.10 Intake.select :close @12:59:45.25 SSV.dial :exhaust @12:59:46.13 Exhaust.select :open @12:59:46.26 SS.reconfigure SSexhale @12:59:46.32 SS.seek 0ml @12:59:55.82 Exhaust.select :close @12:59:55.95 SSV.dial CBV @12:59:55.97 CBV.dial SSV @12:59:56.88 Allowing 130s for 25.0ml of remaining 100.0ml @12:59:56.90 Intake.select :open @12:59:59.09 Intake @15.7psia, vacuum from 9.2 to 13.2psi @12:59:59.11 SS.reconfigure SSinhale.with maxSpeed: 72 @12:59:59.18 SS.seek 25ml @13:00:06.31 Vacuum too high -- waiting up to 40s @13:00:13.33 Intake @15.4psia, vacuum from 8.9 to 12.9psi @13:00:13.35 SS.reconfigure SSinhale.with maxSpeed: 65 @13:00:13.43 SS.seek 25ml @13:00:20.35 Vacuum too high -- waiting up to 40s @13:00:28.03 Intake @15.5psia, vacuum from 9.0 to 13.0psi @13:00:28.05 SS.reconfigure SSinhale.with maxSpeed: 59 @13:00:28.13 SS.seek 25ml @13:00:37.81 Vacuum too high -- waiting up to 40s @13:00:46.55 Intake @15.4psia, vacuum from 8.9 to 12.9psi @13:00:46.57 SS.reconfigure SSinhale.with maxSpeed: 53 @13:00:46.65 SS.seek 25ml @13:00:53.21 Stroke completed -- waiting for pressure to equalize ... @13:01:01.79 Intake.select :close @13:01:01.95 SSV.dial :exhaust @13:01:02.83 Exhaust.select :open @13:01:02.96 SS.reconfigure SSexhale @13:01:03.02 SS.seek 0ml @13:01:12.54 Exhaust.select :close @13:01:12.66 SSV.dial CBV @13:01:12.68 CBV.dial SSV @13:01:13.58 Allowing 130s for 25.0ml of remaining 75.0ml @13:01:13.59 Intake.select :open @13:01:15.79 Intake @15.6psia, vacuum from 9.1 to 13.1psi @13:01:15.81 SS.reconfigure SSinhale.with maxSpeed: 53 @13:01:15.88 SS.seek 25ml @13:01:26.50 Vacuum too high -- waiting up to 40s @13:01:32.60 Intake @15.3psia, vacuum from 8.8 to 12.8psi @13:01:32.62 SS.reconfigure SSinhale.with maxSpeed: 48 @13:01:32.70 SS.seek 25ml @13:01:45.53 Vacuum too high -- waiting up to 40s @13:01:52.75 Intake @15.4psia, vacuum from 8.9 to 12.9psi @13:01:52.77 SS.reconfigure SSinhale.with maxSpeed: 43 @13:01:52.84 SS.seek 25ml @13:02:07.94 Stroke completed -- waiting for pressure to equalize ... @13:02:17.58 Intake.select :close @13:02:17.74 SSV.dial :exhaust @13:02:18.62 Exhaust.select :open @13:02:18.75 SS.reconfigure SSexhale @13:02:18.81 SS.seek 0ml @13:02:28.33 Exhaust.select :close @13:02:28.45 SSV.dial CBV @13:02:28.47 CBV.dial SSV @13:02:29.38 Allowing 130s for 25.0ml of remaining 50.0ml @13:02:29.40 Intake.select :open @13:02:31.59 Intake @15.6psia, vacuum from 9.1 to 13.1psi @13:02:31.61 SS.reconfigure SSinhale.with maxSpeed: 43 @13:02:31.69 SS.seek 25ml @13:02:46.22 delay 5 seconds @13:02:50.10 Vacuum too high -- waiting up to 40s @13:02:51.28 ATV.dial :air2 @13:02:52.36 AS.seek 0.52ml @13:02:55.79 Intake @15.3psia, vacuum from 8.8 to 12.8psi @13:02:55.81 SS.reconfigure SSinhale.with maxSpeed: 39 @13:02:55.88 SS.seek 25ml @13:03:21.43 Stroke completed -- waiting for pressure to equalize ... @13:03:31.83 Intake.select :close @13:03:32.44 SSV.dial :exhaust @13:03:33.35 Exhaust.select :open @13:03:33.48 SS.reconfigure SSexhale @13:03:33.54 SS.seek 0ml @13:03:43.18 Exhaust.select :close @13:03:43.30 SSV.dial CBV @13:03:43.32 CBV.dial SSV @13:03:44.21 Allowing 130s for 25.0ml of remaining 25.0ml @13:03:44.23 Intake.select :open @13:03:46.41 Intake @15.6psia, vacuum from 9.1 to 13.1psi @13:03:46.43 SS.reconfigure SSinhale.with maxSpeed: 39 @13:03:46.51 SS.seek 25ml @13:04:18.31 Vacuum too high -- waiting up to 40s @13:04:24.61 Intake @15.4psia, vacuum from 8.9 to 12.9psi @13:04:24.63 SS.reconfigure SSinhale.with maxSpeed: 35 @13:04:24.71 SS.seek 25ml @13:04:33.22 delay 5 seconds @13:04:38.24 ATV.dial ASV @13:04:39.26 ASV.dial :wasteR @13:04:39.66 AS.seek 0.3ml @13:04:40.02 Stroke completed -- waiting for pressure to equalize ... @13:04:46.78 Intake.select :close @13:04:46.93 SSV.dial :exhaust @13:04:47.82 Exhaust.select :open @13:04:47.94 SS.reconfigure SSexhale @13:04:48.00 SS.seek 0ml @13:04:57.86 Exhaust.select :close @13:04:57.99 Sampled 150.0ml @13:04:58.00 Drying puck before fixing for 4:00 @13:04:58.02 CBV.dial :waste2b @13:04:59.32 delay 1:00 @13:05:24.22 delay 20 seconds @13:05:44.25 AS.seek 0.07ml @13:05:59.41 CSV.dial :air @13:05:59.94 CS.reconfigure CSfast @13:06:00.01 CS.seek 10ml @13:06:26.83 Pumping wcr puck from tube 4 up to 55psi... @13:06:26.84 CBV.dial SSV @13:06:28.30 CBV.dial between CSV and waste2b @13:06:29.42 CSV.dial between air and CTV @13:06:29.92 CS.seek 7.411ml @13:06:30.76 delay 20 seconds @13:06:37.58 CTV.dial CSV,avoiding: :air @13:06:37.61 CSV.dial CTV @13:06:38.52 CS.reconfigure CSpressurize @13:06:38.59 CS.seek 0ml @13:06:50.79 ATV.dial :waste2 @13:06:51.57 AS.reconfigure ASconfig @13:06:51.63 AS.seek :empty @13:06:54.90 CBV.dial :waste2b @13:06:55.28 CS.reconfigure CSconfig @13:06:55.35 CS.seek :empty @13:06:56.03 Elution @13:06:56.04 ATV.dial :air1 @13:06:57.19 AS.reconfigure ASslow1 @13:06:57.25 AS.seek 0.049ml @13:06:59.64 delay 1:00 @13:07:07.66 delay 3 seconds @13:07:10.68 ATV.dial :ElutionWater @13:07:11.38 AS.seek 0.109ml @13:07:23.71 delay 3 seconds @13:07:26.73 ATV.dial ASV,via: :air2 @13:07:28.86 ASV.dial :SPEelution @13:07:29.46 AFV.select :carrierSolution @13:07:29.62 AS.reconfigure ASnorm @13:07:29.68 AS.seek 0.759ml @13:07:59.67 CTV.dial :air,avoiding: :intake @13:08:01.21 Fix #1 of 2 (with rnal) for 2:00 @13:08:01.23 CSV.dial CSR @13:08:01.80 Pulling 2ml of CSR.rnal @13:08:01.82 CSR.select :rnal @13:08:01.96 CS.seek 2ml @13:08:02.76 delay 3 seconds @13:08:05.78 AFV.select :holdingCoil @13:08:05.93 AS.reconfigure ASslow1 @13:08:05.99 AS.seek 0.474ml @13:08:08.24 delay 5 seconds @13:08:13.26 CSR.select :air @13:08:13.39 CS.reconfigure CSfast @13:08:13.45 CS.seek 10ml @13:08:35.02 Pumping wcr puck from tube 4 up to 55psi... @13:08:35.03 CBV.dial SSV @13:08:36.48 CBV.dial between CSV and waste2b @13:08:37.59 CSV.dial between air and CTV @13:08:38.29 CS.seek 7.305ml @13:08:46.24 CTV.dial CSV,avoiding: :air @13:08:46.29 CSV.dial CTV @13:08:47.83 CS.reconfigure CSpressurize @13:08:47.91 CS.seek 0ml @13:09:03.62 delay 5 seconds @13:09:05.95 delay 2:00 @13:09:08.70 AS.reconfigure ASslow2 @13:09:08.77 AS.seek 0.439ml @13:09:44.37 delay 1:00 @13:10:44.40 pushing eluate to holding coil @13:10:44.48 AS.seek 0.279ml @13:11:05.97 CBV.dial :waste2b @13:11:06.35 CS.reconfigure CSconfig @13:11:06.42 CS.seek :empty @13:11:08.68 delay 1:00 @13:12:08.71 CTV.dial :air,avoiding: :intake @13:12:10.24 Fix #2 of 2 (with rnal) for 2:00 @13:12:10.26 CSV.dial CSR @13:12:10.83 Pulling 2ml of CSR.rnal @13:12:10.84 CSR.select :rnal @13:12:10.99 CS.seek 2ml @13:12:17.15 delay 5 seconds @13:12:22.17 CSR.select :air @13:12:22.31 CS.reconfigure CSfast @13:12:22.37 CS.seek 10ml @13:12:43.86 Pumping wcr puck from tube 4 up to 55psi... @13:12:43.87 CBV.dial SSV @13:12:45.33 CBV.dial between CSV and waste2b @13:12:46.46 CSV.dial between air and CTV @13:12:47.30 CS.seek 7.266ml @13:12:55.33 CTV.dial CSV,avoiding: :air @13:12:55.36 CSV.dial CTV @13:12:56.94 CS.reconfigure CSpressurize @13:12:57.01 CS.seek 0ml @13:13:14.51 delay 2:00 @13:13:26.12 delay 30 seconds @13:13:56.15 ATV.dial :waste,via: :air3 @13:13:57.88 AS.reconfigure ASconfig @13:13:57.94 AS.seek :empty @13:14:14.35 Parking Bleach in LysateSource line and SPEcolumn @13:14:14.36 ATV.dial :air3 @13:14:15.22 AS.reconfigure ASslow1 @13:14:15.28 AS.seek 0.029ml @13:14:21.66 ATV.dial :ElutionWater @13:14:22.27 AS.seek 0.059ml @13:14:28.58 ATV.dial :air3 @13:14:29.21 AS.seek 0.069ml @13:14:31.55 delay 2 seconds @13:14:33.57 ATV.dial :bleach @13:14:34.04 AS.seek 0.769ml @13:15:14.53 CBV.dial :waste2b @13:15:14.91 CS.reconfigure CSconfig @13:15:14.98 CS.seek :empty @13:15:17.71 delay 1:00 @13:16:17.73 CTV.dial :air,avoiding: :intake @13:16:19.33 Storing wcr puck in Collection Clamp to tube 3 @13:16:19.51 SC.reconfigure SCtight @13:16:19.57 SC.seek 3 @13:16:21.72 SC.reconfigure SCloose @13:16:21.94 Elbow.reconfigure TightElbow @13:16:22.00 Elbow.seek 3 @13:16:24.00 Forearm.reconfigure TightForearm @13:16:24.06 Forearm.seek 3 + 700 counts @13:16:25.22 SE.reconfigure SEpick @13:16:25.31 SE.seek beamBreak @13:16:28.45 SE.configure SEconfig @13:16:28.61 Elbow.reconfigure LimpElbow @13:16:29.13 Forearm.reconfigure LooseForearm @13:16:29.21 Forearm.seek 3 @13:16:29.54 Elbow.hold @13:16:29.63 Elbow.reconfigure TightElbow @13:16:29.74 SE.seek 1.07 Pucks below Top Plate @13:16:30.37 Forearm.force -20 @13:16:30.43 Forearm.seek 3 + 332 counts @13:16:30.75 Forearm.reconfigure TightForearm @13:16:30.81 Forearm.seek 3 + 700 counts @13:16:31.22 SE.seek 2.07 Pucks below Top Plate @13:16:31.81 Forearm.reconfigure LooseForearm @13:16:31.87 Forearm.seek :retract @13:16:32.82 Elbow.seek CC @13:16:34.52 Elbow.reconfigure LooseElbow @13:16:34.63 Forearm.seek CC @13:16:35.61 Hand.close @13:16:36.39 CC.seek :open @13:16:40.93 Forearm.seek :retract @13:16:41.94 Elbow.reconfigure TightElbow @13:16:42.01 Elbow.seek 3 @13:16:43.91 Elbow.reconfigure LimpElbow @13:16:43.98 Forearm.seek 3 @13:16:45.04 Elbow.hold @13:16:45.12 Elbow.reconfigure TightElbow @13:16:45.18 Hand.open @13:16:46.01 Forearm.force -20 @13:16:46.05 Forearm.seek 3 + 340 counts @13:16:46.37 Forearm.reconfigure TightForearm @13:16:46.43 Forearm.seek 3 + 700 counts @13:16:46.76 SE.seek :down @13:16:47.90 Forearm.reconfigure LooseForearm @13:16:47.96 Forearm.seek :retract @13:16:49.40 SE.coast @13:16:49.45 SC.coast @13:16:49.51 Starting finalPurge @13:16:49.52 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:16:49.62 Hand.open @13:16:50.01 Elbow.seek FlushPuck::Garage @13:16:52.08 Elbow.reconfigure LooseElbow @13:16:52.19 Forearm.seek :garage @13:16:53.21 Hand.close @13:16:54.15 Forearm.seek :retract @13:16:55.08 delay 3 seconds @13:16:55.61
Elbow.reconfigure TightElbow @13:16:55.67 Elbow.seek CC @13:16:58.10 ATV.dial :air3 @13:16:58.64
Elbow.reconfigure LooseElbow @13:16:58.76 delay 3 seconds @13:16:58.79
Forearm.reconfigure TightForearm @13:16:58.87 Forearm.seek CC @13:17:00.14 CC.seek :closed @13:17:01.87 AS.seek 0.794ml @13:17:04.44
Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.33 @13:17:04.54 Hand.open @13:17:05.33 Forearm.reconfigure LooseForearm @13:17:05.39 Forearm.seek :retract @13:17:06.38 Flushing the Collection Valve::Manifold @13:17:06.52 CSV.dial CSR @13:17:07.07 Pulling 5ml of CSR.flush CSR.select :flush @13:17:07.19 delay 3 seconds @13:17:07.23
CS.seek 5ml @13:17:10.21 ATV.dial :bleach @13:17:10.83 AS.seek 0.779ml @13:17:14.12 delay 3 seconds @13:17:17.14 ATV.dial :AIV @13:17:18.41 AS.reconfigure ASconfig @13:17:18.47 AS.seek 0.015ml @13:17:21.34
delay 5 seconds @13:17:26.36 CSR.select :air @13:17:26.53 CS.reconfigure CSfast @13:17:26.59 CS.seek 7.5ml @13:17:34.05 CSV.dial :waste @13:17:35.70 CS.reconfigure CSconfig @13:17:35.76 CS.seek 0ml @13:17:55.90 Flushing lower manifold's waste1 port @13:17:56.04 CSV.dial :flush @13:17:56.57 CS.reconfigure CSfast @13:17:56.64 CS.seek 5ml @13:18:02.71 ATV.dial :waste @13:18:04.23 AS.seek :empty @13:18:05.46 ATV.dial :air3 @13:18:06.14 AS.reconfigure ASslow1 @13:18:06.20 AS.seek 0.009ml @13:18:08.52 delay 2 seconds @13:18:10.56
delay 5 seconds @13:18:10.58 ATV.dial :bleach @13:18:11.05 AS.seek 0.579ml @13:18:15.58
CSV.dial :air @13:18:16.16 CS.seek 7ml @13:18:22.34 CSV.dial CTV @13:18:22.37
CTV.dial CSV @13:18:23.98 CBV.dial :waste1 @13:18:24.86 CS.seek 0ml @13:18:43.68 Flushing lower manifold's waste2b port @13:18:43.82 CSV.dial :flush @13:18:44.71 CS.seek 5ml @13:18:58.68 delay 5 seconds @13:19:03.70 CSV.dial :air @13:19:04.30 CS.seek 6.999ml @13:19:10.81 CSV.dial CTV @13:19:11.30
CTV.dial CSV @13:19:11.85 CBV.dial :waste2b @13:19:12.75 CS.seek 0ml @13:19:31.68 Flushing lower manifold's waste1 port @13:19:31.69 CSV.dial CSR @13:19:32.27 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @13:19:32.42 CS.reconfigure CSconfig @13:19:32.48 CS.seek 4ml @13:19:43.74 delay 5 seconds @13:19:48.76 CSR.select :air @13:19:48.90 CS.reconfigure CSfast @13:19:48.96 CS.seek 10ml @13:20:05.24 CSV.dial CTV @13:20:05.27
CTV.dial CSV @13:20:05.81 CBV.dial :waste1 @13:20:05.96 delay 3 seconds @13:20:06.72
CS.seek 0ml @13:20:08.98 ATV.dial :air3 @13:20:09.55 delay 3 seconds @13:20:12.62 AS.seek 0.604ml @13:20:18.02 delay 3 seconds @13:20:21.04 ATV.dial :bleach @13:20:21.66 AS.seek 0.589ml @13:20:24.98 delay 3 seconds @13:20:28.00 ATV.dial :ASV @13:20:29.07 ASV.dial :wasteR @13:20:29.69 AS.seek 0ml @13:20:33.31
Flushing lower manifold's waste2b port @13:20:33.32 CSV.dial CSR @13:20:33.93 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @13:20:34.08 CS.reconfigure CSconfig @13:20:34.14 CS.seek 4ml @13:20:45.37 delay 5 seconds @13:20:50.39 CSR.select :air @13:20:50.53 CS.reconfigure CSfast @13:20:50.59 CS.seek 10ml @13:21:06.87 CSV.dial CTV @13:21:06.92
CTV.dial CSV @13:21:07.42 CBV.dial :waste2b @13:21:08.32 CS.seek 0ml @13:21:34.90 Killing upper manifold @13:21:34.91 CSV.dial CSR @13:21:35.51 Pulling 4ml of CSR.mfbkill @13:21:35.52 CSR.select :mfbkill @13:21:35.66 CS.reconfigure CSconfig @13:21:35.72 CS.seek 4ml @13:21:46.93 delay 5 seconds @13:21:51.95 CSR.select :air @13:21:52.09 CS.reconfigure CSfast @13:21:52.15 CS.seek 10ml @13:22:08.43 CSV.dial CBV @13:22:08.46
CBV.dial CSV @13:22:09.68 CTV.dial :air @13:22:11.22 CS.seek 0ml @13:22:28.46 delay 1:00 @13:22:37.71
delay 7 seconds @13:22:44.75 CS.seek 3ml @13:22:53.37 CSV.dial :waste @13:22:53.90 CS.seek 0ml @13:23:02.50 Flushing upper manifold @13:23:02.63 CSV.dial :flush @13:23:03.16 CS.seek 5ml @13:23:17.00 delay 5 seconds @13:23:22.02 CSV.dial :air @13:23:22.59 CS.seek 7ml @13:23:28.48 AFV.select :carrierSolution @13:23:28.59 delay 2 seconds @13:23:28.75 CSV.dial CBV @13:23:28.78
CBV.dial CSV @13:23:30.00 CTV.dial :air @13:23:30.02 CS.seek 0ml @13:23:30.62 AS.reconfigure ASconfig @13:23:30.67 AS.seek 1ml @13:23:49.09
delay 7 seconds @13:23:56.12 CS.seek 3ml @13:24:04.87 CSV.dial :waste @13:24:05.41 CS.seek 0ml @13:24:14.60 CSV.dial :flush @13:24:15.13 CS.seek 3ml @13:24:23.87 delay 5 seconds @13:24:28.46 delay 1 second @13:24:28.89
CSV.dial :air @13:24:29.46 CS.seek 5ml @13:24:29.53 AFV.select :holdingCoil @13:24:29.64 delay 1 second @13:24:30.66 ATV.dial ASV @13:24:30.67 ASV.dial :wasteR @13:24:30.70 AS.reconfigure ASslow1 @13:24:30.77 AS.seek 0ml @13:24:35.59
CSV.dial :air @13:24:35.61 CS.seek 0ml @13:24:49.30 delay 7 seconds @13:24:56.34 CS.seek 3ml @13:25:05.09 CSV.dial :waste @13:25:05.96 CS.seek 0ml @13:25:14.65 Flushing lower manifold's waste1 port @13:25:14.79 CSV.dial :flush @13:25:15.34 CS.seek 5ml @13:25:29.15 delay 5 seconds @13:25:34.17 CSV.dial :air @13:25:34.75 CS.seek 7.001ml @13:25:40.94 CSV.dial CTV @13:25:40.97
CTV.dial CSV @13:25:42.56 CBV.dial :waste1 @13:25:43.09 CS.seek 0ml @13:26:01.81 Flushing lower manifold's waste2b port @13:26:01.95 CSV.dial :flush @13:26:02.84 CS.seek 5ml @13:26:16.62 delay 5 seconds @13:26:21.64 CSV.dial :air @13:26:22.25 CS.seek 7ml @13:26:28.31 CSV.dial CTV @13:26:28.33
CTV.dial CSV @13:26:28.86 CBV.dial :waste2b @13:26:29.76 CS.seek 0ml @13:26:48.44 Garaging FlushPuck in Collection Clamp @13:26:48.53 Elbow.reconfigure TightElbow @13:26:48.59 Elbow.seek CC @13:26:48.81 Elbow.reconfigure LooseElbow @13:26:48.91 Forearm.seek CC @13:26:49.88 Hand.close @13:26:50.66 CC.seek :open @13:26:55.05 Forearm.seek :retract @13:26:56.05 Elbow.reconfigure TightElbow @13:26:56.11 Elbow.seek :garage @13:26:59.19 Elbow.reconfigure LooseElbow @13:26:59.30 Forearm.reconfigure TightForearm @13:26:59.37 Forearm.seek :garage @13:27:00.55 Hand.open @13:27:01.34 Forearm.reconfigure LooseForearm @13:27:01.40 Forearm.seek :retract @13:27:02.44 Starting initialPurge @13:27:02.45 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @13:27:02.54 Hand.open @13:27:02.84 Elbow.reconfigure TightElbow @13:27:02.90 Elbow.seek FlushPuck::Garage @13:27:03.03 Elbow.reconfigure LooseElbow @13:27:03.14 Forearm.seek :garage @13:27:04.19 Hand.close @13:27:05.03 Forearm.seek :retract @13:27:06.53 Elbow.reconfigure TightElbow @13:27:06.59 Elbow.seek CC @13:27:09.40 Elbow.reconfigure LooseElbow @13:27:09.50 Forearm.reconfigure TightForearm @13:27:09.56 Forearm.seek CC @13:27:10.81 CC.seek :closed @13:27:15.08 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.37 @13:27:15.16 Hand.open @13:27:15.98 Forearm.reconfigure LooseForearm @13:27:16.04 Forearm.seek :retract @13:27:17.05 Flushing the Collection Valve::Manifold @13:27:17.19 CSV.dial CSR @13:27:17.75 Pulling 5ml of CSR.flush @13:27:17.76 CSR.select :flush @13:27:17.90 CS.reconfigure CSconfig @13:27:17.96 CS.seek 5ml @13:27:31.81 delay 5 seconds @13:27:36.83 CSR.select :air @13:27:37.00 CS.reconfigure CSfast @13:27:37.06 CS.seek 7.5ml @13:27:44.50 CSV.dial :waste @13:27:46.01 CS.reconfigure CSconfig @13:27:46.08 CS.seek 0ml @13:27:52.18 delay 20 seconds @13:28:06.41
Purging external Intake @13:28:06.54 CSV.dial :flush @13:28:07.05 CS.reconfigure CSfast @13:28:07.11 CS.seek 10ml @13:28:12.20 ATV.dial :air3 @13:28:13.74 AS.seek 0.59ml @13:28:33.72
delay 5 seconds @13:28:38.74 CSV.dial CBV @13:28:38.78
CBV.dial CSV @13:28:39.72 CTV.dial :intake @13:28:40.59 Intake.select :open @13:28:40.72 CS.seek 0ml @13:29:08.31 Intake.select :close @13:29:08.42 CTV.dial :air @13:29:09.28 Flushing upper manifold @13:29:09.41 CSV.dial :flush @13:29:10.28 CS.seek 5ml @13:29:24.09 delay 5 seconds @13:29:29.11 CSV.dial :air @13:29:29.70 CS.seek 7ml @13:29:35.81 CSV.dial CBV @13:29:35.84
CBV.dial CSV @13:29:37.05 CTV.dial :air @13:29:37.07 CS.seek 0ml @13:29:55.78 delay 7 seconds @13:30:02.82 CS.seek 3ml @13:30:11.50 CSV.dial :waste @13:30:12.04 CS.seek 0ml @13:30:12.66 delay 3 seconds @13:30:15.68 ATV.dial :ASV @13:30:17.15 ASV.dial :wasteR @13:30:17.23 AS.seek 0.09ml @13:30:20.69
CSV.dial :flush @13:30:21.20 CS.seek 3ml @13:30:29.88 delay 5 seconds @13:30:34.90 CSV.dial :air @13:30:35.48 CS.seek 5ml @13:30:41.47 CSV.dial :air @13:30:41.49 CS.seek 0ml @13:30:55.13 delay 7 seconds @13:31:02.16 CS.seek 3ml @13:31:10.72 CSV.dial :waste @13:31:11.61 CS.seek 0ml @13:31:20.64 Garaging FlushPuck in Collection Clamp @13:31:20.72 Elbow.reconfigure TightElbow @13:31:20.78 Elbow.seek CC @13:31:20.91 Elbow.reconfigure LooseElbow @13:31:21.02 Forearm.seek CC @13:31:21.99 Hand.close @13:31:22.79 CC.seek :open @13:31:27.18 Forearm.seek :retract @13:31:28.16 Elbow.reconfigure TightElbow @13:31:28.22 Elbow.seek :garage @13:31:31.29 Elbow.reconfigure LooseElbow @13:31:31.40 Forearm.reconfigure TightForearm @13:31:31.46 Forearm.seek :garage @13:31:32.65 Hand.open @13:31:33.47 Forearm.reconfigure LooseForearm @13:31:33.53 Forearm.seek :retract @13:31:34.60 Completed [mbaPhase] begun 11:56:13 @13:31:34.62 Extending 18tun_postDeploy2 until completion of MFBPCR @13:31:58.07 delay 30 seconds @13:32:28.10 ATV.dial :extSrc @13:32:28.87 delay 3 seconds @13:32:31.89 ATV.dial :ElutionWater @13:32:33.00 AS.seek 0.12ml @13:32:39.35 ATV.dial :air3 @13:32:39.97 AS.seek 0.13ml @13:32:42.27 delay 2 seconds @13:32:44.29 ATV.dial :bleach @13:32:44.75 AS.seek 0.23ml @13:33:05.15 delay 3 seconds @13:33:08.17 ATV.dial :air3 @13:33:08.74 delay 3 seconds @13:33:11.81 AS.seek 0.255ml @13:33:17.16 delay 3 seconds @13:33:20.18 ATV.dial :bleach @13:33:20.80 AS.seek 0.24ml @13:33:24.12 delay 3 seconds @13:33:27.14 ATV.dial :ASV @13:33:28.20 ASV.dial :SPEdelivery @13:33:28.59 AFV.select :carrierSolution @13:33:28.69 delay 2 seconds @13:33:30.72 AS.reconfigure ASconfig @13:33:30.77 AS.seek 0.5ml @13:33:46.02 delay 1 second @13:33:47.04 AFV.select :holdingCoil @13:33:47.19 AS.reconfigure ASslow1 @13:33:47.25 AS.seek 0.17ml @13:34:53.89 SPE.hold 2:46:40,95C @13:34:53.96 delay 3:00 @13:37:53.98 AFV.select :carrierSolution @13:37:54.09 delay 2 seconds @13:37:56.12 AS.reconfigure ASconfig @13:37:56.17 AS.seek 1ml @13:38:44.16 delay 1 second @13:38:45.18 AFV.select :holdingCoil @13:38:45.28 delay 1 second @13:38:46.30 ASV.dial :wasteD @13:38:47.49 AS.seek 0ml @13:39:45.29 Priming Eluate @13:39:45.30 AFV.select :carrierSolution @13:39:45.41 delay 2 seconds @13:39:47.44 AS.seek 0.5ml @13:40:16.46 delay 1 second @13:40:17.48 AFV.select :holdingCoil @13:40:17.58 ATV.dial ASV @13:40:17.59 ASV.dial :SPErecovery @13:40:18.23 Swish #1 of 2 @13:40:18.31 AS.reconfigure ASslow0 @13:40:18.37 AS.seek 0.6ml @13:40:28.80 AS.seek 0.5ml @13:40:39.20 Swish #2 of 2 @13:40:39.28 AS.seek 0.6ml @13:40:49.71 AS.seek 0.5ml @13:41:00.09 delay 3 seconds @13:41:03.16 AS.reconfigure ASslow1 @13:41:03.22 AS.seek 0.684ml @13:41:40.54 delay 3 seconds @13:41:43.56 Eluate primed @13:41:43.57 ATV.dial :waste2 @13:41:44.20 AS.reconfigure ASnorm @13:41:44.27 AS.seek 0ml @13:42:19.09 SPE.coast @13:42:19.18 ASV.dial :SPErecovery @13:42:19.20 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118nov2113h150ml.pcr @13:42:19.22 Building PCR Slug from ASV with MM2 mix, PP4 primer @13:42:19.24 Priming with carrier @13:42:19.26 ATV.dial ARV @13:42:20.26 ARV.dial :carrier @13:42:21.09 AS.seek 0.5ml @13:42:46.68 AFV.select :carrierSolution @13:42:46.80 delay 2 seconds @13:42:48.83 AS.reconfigure ASconfig @13:42:48.89 AS.seek 1ml @13:43:17.91 delay 1 second @13:43:18.93 AFV.select :holdingCoil @13:43:19.03 delay 1 second @13:43:20.05 ATV.dial :waste @13:43:21.54 AS.reconfigure ASnorm @13:43:21.61 AS.seek :empty @13:44:12.37 AFV.select :carrierSolution @13:44:12.49 delay 2 seconds @13:44:14.52 AS.reconfigure ASconfig @13:44:14.58 AS.seek 0.5ml @13:44:43.71 delay 1 second @13:44:44.73 AFV.select :holdingCoil @13:44:44.84 Building Slug @13:44:44.85 ATV.dial :ARV @13:44:46.48 delay 2 seconds @13:44:48.50 ARV.dial :air1 @13:44:48.91 delay 2 seconds @13:44:50.98 AS.reconfigure ASslow2 @13:44:51.05 AS.seek 0.506ml @13:44:57.34 delay 2 seconds @13:44:59.36 ARV.dial :MM2 @13:45:00.88 delay 2 seconds @13:45:02.90 Adding MM2 @13:45:02.99 AS.seek 0.524ml @13:45:21.27 delay 25 seconds @13:45:46.29 ARV.dial :PP4 @13:45:46.70 Adding PP4 @13:45:46.71 delay 2 seconds @13:45:48.79 AS.seek 0.53ml @13:45:54.95 delay 10 seconds @13:46:04.98 ARV.dial :air3 @13:46:05.38 adding ARV air @13:46:05.39 delay 2 seconds @13:46:07.47 AS.seek 0.536ml @13:46:13.74 delay 5 seconds @13:46:18.76 ARV.dial :carrier @13:46:20.06 delay 7 seconds @13:46:27.15 AS.seek 0.594ml @13:47:25.65 delay 5 seconds @13:47:30.67 ATV.dial :ASV @13:47:31.09 delay 2 seconds @13:47:33.16 AS.seek 0.6ml @13:47:39.35 delay 4 seconds @13:47:43.37 ATV.dial ARV @13:47:43.93 delay 2 seconds @13:47:45.95 ARV.dial :carrier @13:47:45.96 delay 2 seconds @13:47:48.04 AS.seek 0.65ml @13:48:38.49 delay 4 seconds @13:48:42.51 ATV.dial PCR @13:48:43.12 ASV.dial :waste1 @13:48:43.92 ARV.dial :air1 @13:48:44.34 Positioning Slug by 0.324ml @13:48:44.44 AS.seek 0.326ml @13:54:10.49 delay 5 seconds @13:54:15.51 ATV.dial ARV @13:54:16.27 ASV.dial :PCRlock @13:54:16.66 delay 5 seconds @13:54:21.68 Running Tt1 Profile60C300 thermocycling @13:54:21.70 PCR.program Profile60C300 @13:54:21.87 Profile60C300 should complete in < 1:45:21 @13:54:21.88 PCR.run 2:16:41.25 @15:16:29.51 PCR.off @15:16:31.01 PCR for Tt1 completed -- closed /var/log/esp/Tt118nov2113h150ml.pcr @15:16:31.09 Cleaning PCR @15:16:31.10 AFV.select :carrierSolution @15:16:31.22 delay 2 seconds @15:16:33.25 AS.reconfigure ASconfig @15:16:33.30 AS.seek 0.5ml @15:16:43.62 delay 1 second @15:16:44.64 AFV.select :holdingCoil @15:16:44.74 ASV.dial :waste1 @15:16:45.14 ATV.dial :air3 @15:16:46.42 AS.reconfigure ASslow1 @15:16:46.48 AS.seek 0.51ml @15:16:48.79 delay 2 seconds @15:16:50.81 ATV.dial :bleach @15:16:51.43 AS.seek 0.81ml @15:17:52.04 delay 3 seconds @15:17:55.06 ATV.dial :air3 @15:17:55.62 delay 3 seconds @15:17:58.69 AS.seek 0.835ml @15:18:04.01 delay 3 seconds @15:18:07.03 ATV.dial :bleach @15:18:07.65 AS.seek 0.82ml @15:18:10.97 delay 3 seconds @15:18:13.99 ARV.dial :waste @15:18:14.62 delay 3 seconds @15:18:17.64 ATV.dial :ARV @15:18:19.03 AS.reconfigure ASconfig @15:18:19.09 AS.seek 0.47ml @15:18:39.51 AFV.select :carrierSolution @15:18:39.62 delay 2 seconds @15:18:41.65 AS.seek 1ml @15:19:12.42 delay 1 second @15:19:13.44 AFV.select :holdingCoil @15:19:13.54 delay 1 second @15:19:14.57 AS.reconfigure ASfast @15:19:14.63 AS.seek 0ml @15:19:41.98 AFV.select :carrierSolution @15:19:42.10 delay 2 seconds @15:19:44.13 AS.reconfigure ASconfig @15:19:44.19 AS.seek 1ml @15:20:41.95 delay 1 second @15:20:42.97 AFV.select :holdingCoil @15:20:43.07 delay 1 second @15:20:44.10 AS.reconfigure ASfast @15:20:44.15 AS.seek 0ml @15:21:11.50 AFV.select :carrierSolution @15:21:11.62 delay 2 seconds @15:21:13.65 AS.reconfigure ASconfig @15:21:13.70 AS.seek 1ml @15:22:11.51 delay 1 second @15:22:12.53 AFV.select :holdingCoil @15:22:12.63 delay 1 second @15:22:13.66 AS.reconfigure ASfast @15:22:13.72 AS.seek 0ml @15:22:41.09 ARV.dial between carrier and air1 @15:22:41.59 ATV.dial :air3 @15:22:42.89 AS.reconfigure ASslow1 @15:22:42.95 AS.seek 0.01ml @15:22:45.28 delay 2 seconds @15:22:47.30 ATV.dial :bleach @15:22:47.92 AS.seek 0.81ml @15:25:29.17 delay 3 seconds @15:25:32.19 ATV.dial :air3 @15:25:32.75 delay 3 seconds @15:25:35.83 AS.seek 0.835ml @15:25:41.16 delay 3 seconds @15:25:44.18 ATV.dial :bleach @15:25:44.81 AS.seek 0.82ml @15:25:48.12 delay 3 seconds @15:25:51.14 ATV.dial PCR @15:25:51.54 AS.reconfigure ASconfig @15:25:51.60 AS.seek :empty @15:26:39.09 PCR.program :bleachProfile @15:26:39.15 PCR program should complete in < 2:45 @15:26:39.16 PCR.run 10:00 @15:29:37.96 PCR.off @15:29:37.99 AFV.select :carrierSolution @15:29:38.11 delay 2 seconds @15:29:40.14 AS.seek 1ml @15:30:37.97 delay 1 second @15:30:38.99 AFV.select :holdingCoil @15:30:39.09 delay 1 second @15:30:40.12 AS.reconfigure ASfast @15:30:40.17 AS.seek 0ml @15:31:07.51 delay 3 seconds @15:31:10.53 AFV.select :carrierSolution @15:31:10.63 delay 2 seconds @15:31:12.66 AS.reconfigure ASconfig @15:31:12.72 AS.seek 1ml @15:32:10.53 delay 1 second @15:32:11.55 AFV.select :holdingCoil @15:32:11.65 delay 1 second @15:32:12.68 AS.reconfigure ASfast @15:32:12.73 AS.seek 0ml @15:32:40.06 delay 3 seconds @15:32:43.08 AFV.select :carrierSolution @15:32:43.18 delay 2 seconds @15:32:45.21 AS.reconfigure ASconfig @15:32:45.27 AS.seek 1ml @15:33:43.06 delay 1 second @15:33:44.08 AFV.select :holdingCoil @15:33:44.18 delay 1 second @15:33:45.21 AS.reconfigure ASfast @15:33:45.26 AS.seek 0ml @15:34:12.61 delay 3 seconds @15:34:15.63 AFV.select :carrierSolution @15:34:15.73 delay 2 seconds @15:34:17.76 AS.reconfigure ASconfig @15:34:17.82 AS.seek 1ml @15:35:15.59 delay 1 second @15:35:16.61 AFV.select :holdingCoil @15:35:16.71 delay 1 second @15:35:17.74 AS.reconfigure ASfast @15:35:17.79 AS.seek 0ml @15:35:45.14 delay 3 seconds @15:35:48.16 AFV.select :carrierSolution @15:35:48.26 delay 2 seconds @15:35:50.29 AS.reconfigure ASconfig @15:35:50.35 AS.seek 1ml @15:36:48.09 delay 1 second @15:36:49.11 AFV.select :holdingCoil @15:36:49.21 delay 1 second @15:36:50.24 AS.reconfigure ASnorm @15:36:50.29 AS.seek 0ml @15:37:41.03 Setting up PCR for Rp -- writing /var/log/esp/Rp18nov2115h150ml.pcr @15:37:41.04 Building PCR Slug from ASV with MM2 mix, PP3 primer @15:37:41.06 Priming with carrier @15:37:41.09 ATV.dial ARV @15:37:42.22 ARV.dial :carrier @15:37:42.56 AS.seek 0.5ml @15:38:08.12 AFV.select :carrierSolution @15:38:08.23 delay 2 seconds @15:38:10.26 AS.reconfigure ASconfig @15:38:10.32 AS.seek 1ml @15:38:39.34 delay 1 second @15:38:40.36 AFV.select :holdingCoil @15:38:40.46 delay 1 second @15:38:41.48 ATV.dial :waste @15:38:43.14 AS.reconfigure ASnorm @15:38:43.21 AS.seek :empty @15:39:34.00 AFV.select :carrierSolution @15:39:34.12 delay 2 seconds @15:39:36.15 AS.reconfigure ASconfig @15:39:36.21 AS.seek 0.5ml @15:40:05.33 delay 1 second @15:40:06.35 AFV.select :holdingCoil @15:40:06.45 Building Slug @15:40:06.47 ATV.dial :ARV @15:40:08.12 delay 2 seconds @15:40:10.14 ARV.dial :air1 @15:40:10.64 delay 2 seconds @15:40:12.71 AS.reconfigure ASslow2 @15:40:12.78 AS.seek 0.506ml @15:40:19.08 delay 2 seconds @15:40:21.10 ARV.dial :MM2 @15:40:22.62 delay 2 seconds @15:40:24.64 Adding MM2 @15:40:24.73 AS.seek 0.524ml @15:40:43.01 delay 25 seconds @15:41:08.03 ARV.dial :PP3 @15:41:08.44 Adding PP3 @15:41:08.46 delay 2 seconds @15:41:10.53 AS.seek 0.53ml @15:41:16.70 delay 10 seconds @15:41:26.73 ARV.dial :air2 @15:41:27.12 adding ARV air @15:41:27.13 delay 2 seconds @15:41:29.21 AS.seek 0.536ml @15:41:35.44 delay 5 seconds @15:41:40.46 ARV.dial :carrier @15:41:41.76 delay 7 seconds @15:41:48.84 AS.seek 0.594ml @15:42:47.36 delay 5 seconds @15:42:52.38 ATV.dial :ASV @15:42:52.78 delay 2 seconds @15:42:54.85 AS.seek 0.6ml @15:43:01.09 delay 4 seconds @15:43:05.11 ATV.dial ARV @15:43:05.76 delay 2 seconds @15:43:07.78 ARV.dial :carrier @15:43:07.79 delay 2 seconds @15:43:09.87 AS.seek 0.65ml @15:44:00.41 delay 4 seconds @15:44:04.43 ATV.dial PCR @15:44:05.05 ASV.dial :waste1 @15:44:05.06 ARV.dial :air1 @15:44:05.49 Positioning Slug by 0.324ml @15:44:05.59 AS.seek 0.326ml @15:49:31.70 delay 5 seconds @15:49:36.72 ATV.dial ARV @15:49:37.50 ASV.dial :PCRlock @15:49:37.91 delay 5 seconds @15:49:42.93 Running Rp Profile60C300 thermocycling @15:49:42.95 PCR.program Profile60C300 @15:49:43.09 Profile60C300 should complete in < 1:45:21 @15:49:43.10 PCR.run 2:16:41.25 @17:11:43.17 PCR.off @17:11:44.69 PCR for Rp completed -- closed /var/log/esp/Rp18nov2115h150ml.pcr @17:11:44.77 Cleaning PCR @17:11:44.78 AFV.select :carrierSolution @17:11:44.90 delay 2 seconds @17:11:46.93 AS.reconfigure ASconfig @17:11:46.98 AS.seek 0.5ml @17:11:57.29 delay 1 second @17:11:58.31 AFV.select :holdingCoil @17:11:58.41 ASV.dial :waste1 @17:11:58.82 ATV.dial :air3 @17:12:00.11 AS.reconfigure ASslow1 @17:12:00.17 AS.seek 0.51ml @17:12:02.46 delay 2 seconds @17:12:04.48 ATV.dial :bleach @17:12:05.10 AS.seek 0.81ml @17:13:05.79 delay 3 seconds @17:13:08.81 ATV.dial :air3 @17:13:09.36 delay 3 seconds @17:13:12.43 AS.seek 0.835ml @17:13:17.75 delay 3 seconds @17:13:20.77 ATV.dial :bleach @17:13:21.39 AS.seek 0.82ml @17:13:24.72 delay 3 seconds @17:13:27.74 ARV.dial :waste @17:13:28.38 delay 3 seconds @17:13:31.40 ATV.dial :ARV @17:13:32.27 AS.reconfigure ASconfig @17:13:32.33 AS.seek 0.47ml @17:13:52.77 AFV.select :carrierSolution @17:13:52.88 delay 2 seconds @17:13:54.91 AS.seek 1ml @17:14:26.19 delay 1 second @17:14:27.21 AFV.select :holdingCoil @17:14:27.31 delay 1 second @17:14:28.34 AS.reconfigure ASfast @17:14:28.39 AS.seek 0ml @17:14:55.74 AFV.select :carrierSolution @17:14:55.85 delay 2 seconds @17:14:57.88 AS.reconfigure ASconfig @17:14:57.94 AS.seek 1ml @17:15:55.71 delay 1 second @17:15:56.73 AFV.select :holdingCoil @17:15:56.83 delay 1 second @17:15:57.86 AS.reconfigure ASfast @17:15:57.91 AS.seek 0ml @17:16:25.27 AFV.select :carrierSolution @17:16:25.38 delay 2 seconds @17:16:27.41 AS.reconfigure ASconfig @17:16:27.46 AS.seek 1ml @17:17:25.29 delay 1 second @17:17:26.31 AFV.select :holdingCoil @17:17:26.41 delay 1 second @17:17:27.44 AS.reconfigure ASfast @17:17:27.49 AS.seek 0ml @17:17:54.86 ARV.dial between carrier and air1 @17:17:55.38 ATV.dial :air3 @17:17:56.66 AS.reconfigure ASslow1 @17:17:56.72 AS.seek 0.01ml @17:17:59.02 delay 2 seconds @17:18:01.04 ATV.dial :bleach @17:18:01.66 AS.seek 0.81ml @17:20:42.82 delay 3 seconds @17:20:45.84 ATV.dial :air3 @17:20:46.41 delay 3 seconds @17:20:49.48 AS.seek 0.835ml @17:20:54.82 delay 3 seconds @17:20:57.84 ATV.dial :bleach @17:20:58.47 AS.seek 0.82ml @17:21:01.79 delay 3 seconds @17:21:04.81 ATV.dial PCR @17:21:05.22 AS.reconfigure ASconfig @17:21:05.28 AS.seek :empty @17:21:52.77 PCR.program :bleachProfile @17:21:52.82 PCR program should complete in < 2:45 @17:21:52.83 PCR.run 10:00 @17:24:51.63 PCR.off @17:24:51.66 AFV.select :carrierSolution @17:24:51.78 delay 2 seconds @17:24:53.81 AS.seek 1ml @17:25:51.65 delay 1 second @17:25:52.67 AFV.select :holdingCoil @17:25:52.77 delay 1 second @17:25:53.80 AS.reconfigure ASfast @17:25:53.85 AS.seek 0ml @17:26:21.21 delay 3 seconds @17:26:24.23 AFV.select :carrierSolution @17:26:24.33 delay 2 seconds @17:26:26.37 AS.reconfigure ASconfig @17:26:26.42 AS.seek 1ml @17:27:24.18 delay 1 second @17:27:25.20 AFV.select :holdingCoil @17:27:25.30 delay 1 second @17:27:26.33 AS.reconfigure ASfast @17:27:26.38 AS.seek 0ml @17:27:53.72 delay 3 seconds @17:27:56.74 AFV.select :carrierSolution @17:27:56.84 delay 2 seconds @17:27:58.87 AS.reconfigure ASconfig @17:27:58.93 AS.seek 1ml @17:28:56.71 delay 1 second @17:28:57.73 AFV.select :holdingCoil @17:28:57.83 delay 1 second @17:28:58.86 AS.reconfigure ASfast @17:28:58.91 AS.seek 0ml @17:29:26.26 delay 3 seconds @17:29:29.28 AFV.select :carrierSolution @17:29:29.38 delay 2 seconds @17:29:31.41 AS.reconfigure ASconfig @17:29:31.46 AS.seek 1ml @17:30:29.26 delay 1 second @17:30:30.28 AFV.select :holdingCoil @17:30:30.38 delay 1 second @17:30:31.41 AS.reconfigure ASfast @17:30:31.46 AS.seek 0ml @17:30:58.83 delay 3 seconds @17:31:01.85 AFV.select :carrierSolution @17:31:01.95 delay 2 seconds @17:31:03.98 AS.reconfigure ASconfig @17:31:04.04 AS.seek 1ml @17:32:01.83 delay 1 second @17:32:02.85 AFV.select :holdingCoil @17:32:02.96 delay 1 second @17:32:03.99 AS.reconfigure ASnorm @17:32:04.04 AS.seek 0ml @17:32:54.78 Setting up PCR for Bb -- writing /var/log/esp/Bb18nov2117h150ml.pcr @17:32:54.79 Building PCR Slug from ASV with MM3 mix, PP5 primer @17:32:54.82 Priming with carrier @17:32:54.84 ATV.dial ARV @17:32:55.46 ARV.dial :carrier @17:32:55.82 AS.seek 0.5ml @17:33:21.40 AFV.select :carrierSolution @17:33:21.51 delay 2 seconds @17:33:24.03 AS.reconfigure ASconfig @17:33:24.11 AS.seek 1ml @17:33:53.18 delay 1 second @17:33:54.20 AFV.select :holdingCoil @17:33:54.30 delay 1 second @17:33:55.32 ATV.dial :waste @17:33:56.97 AS.reconfigure ASnorm @17:33:57.04 AS.seek :empty @17:34:47.80 AFV.select :carrierSolution @17:34:47.92 delay 2 seconds @17:34:49.95 AS.reconfigure ASconfig @17:34:50.01 AS.seek 0.5ml @17:35:19.15 delay 1 second @17:35:20.17 AFV.select :holdingCoil @17:35:20.27 Building Slug @17:35:20.29 ATV.dial :ARV @17:35:22.04 delay 2 seconds @17:35:24.06 ARV.dial :air1 @17:35:24.46 delay 2 seconds @17:35:26.53 AS.reconfigure ASslow2 @17:35:26.60 AS.seek 0.506ml @17:35:33.22 delay 2 seconds @17:35:35.24 ARV.dial :MM3 @17:35:36.32 delay 2 seconds @17:35:38.34 Adding MM3 @17:35:38.43 AS.seek 0.524ml @17:35:56.71 delay 25 seconds @17:36:21.73 ARV.dial :PP5 @17:36:22.13 Adding PP5 @17:36:22.14 delay 2 seconds @17:36:24.22 AS.seek 0.53ml @17:36:30.41 delay 10 seconds @17:36:40.44 ARV.dial :air3 @17:36:40.85 adding ARV air @17:36:40.86 delay 2 seconds @17:36:42.94 AS.seek 0.536ml @17:36:49.33 delay 5 seconds @17:36:54.35 ARV.dial :carrier @17:36:55.65 delay 7 seconds @17:37:02.73 AS.seek 0.594ml @17:38:02.11 delay 5 seconds @17:38:07.13 ATV.dial :ASV @17:38:07.54 delay 2 seconds @17:38:09.61 AS.seek 0.6ml @17:38:15.87 delay 4 seconds @17:38:19.89 ATV.dial ARV @17:38:20.44 delay 2 seconds @17:38:22.46 ARV.dial :carrier @17:38:22.47 delay 2 seconds @17:38:24.55 AS.seek 0.65ml @17:39:15.08 delay 4 seconds @17:39:19.10 ATV.dial PCR @17:39:19.71 ASV.dial :waste1 @17:39:19.72 ARV.dial :air1 @17:39:20.16 Positioning Slug by 0.324ml @17:39:20.25 AS.seek 0.326ml @17:44:46.08 delay 5 seconds @17:44:51.10 ATV.dial ARV @17:44:51.87 ASV.dial :PCRlock @17:44:52.27 delay 5 seconds @17:44:57.29 Running Bb Profile60C300 thermocycling @17:44:57.31 PCR.program Profile60C300 @17:44:57.45 Profile60C300 should complete in < 1:45:21 @17:44:57.46 PCR.run 2:16:41.25 @19:06:51.98 PCR.off @19:06:53.50 PCR for Bb completed -- closed /var/log/esp/Bb18nov2117h150ml.pcr @19:06:53.58 Cleaning PCR @19:06:53.59 AFV.select :carrierSolution @19:06:53.71 delay 2 seconds @19:06:55.74 AS.reconfigure ASconfig @19:06:55.79 AS.seek 0.5ml @19:07:06.09 delay 1 second @19:07:07.11 AFV.select :holdingCoil @19:07:07.21 ASV.dial :waste1 @19:07:07.61 ATV.dial :air3 @19:07:08.90 AS.reconfigure ASslow1 @19:07:08.96 AS.seek 0.51ml @19:07:11.22 delay 2 seconds @19:07:13.24 ATV.dial :bleach @19:07:13.87 AS.seek 0.81ml @19:08:14.50 delay 3 seconds @19:08:17.52 ATV.dial :air3 @19:08:18.09 delay 3 seconds @19:08:21.16 AS.seek 0.835ml @19:08:26.46 delay 3 seconds @19:08:29.48 ATV.dial :bleach @19:08:30.10 AS.seek 0.82ml @19:08:33.40 delay 3 seconds @19:08:36.42 ARV.dial :waste @19:08:37.04 delay 3 seconds @19:08:40.06 ATV.dial :ARV @19:08:40.94 AS.reconfigure ASconfig @19:08:41.01 AS.seek 0.47ml @19:09:01.42 AFV.select :carrierSolution @19:09:01.53 delay 2 seconds @19:09:03.56 AS.seek 1ml @19:09:34.32 delay 1 second @19:09:35.34 AFV.select :holdingCoil @19:09:35.44 delay 1 second @19:09:36.47 AS.reconfigure ASfast @19:09:36.52 AS.seek 0ml @19:10:03.89 AFV.select :carrierSolution @19:10:04.00 delay 2 seconds @19:10:06.03 AS.reconfigure ASconfig @19:10:06.08 AS.seek 1ml @19:11:03.86 delay 1 second @19:11:04.88 AFV.select :holdingCoil @19:11:04.98 delay 1 second @19:11:06.01 AS.reconfigure ASfast @19:11:06.06 AS.seek 0ml @19:11:33.42 AFV.select :carrierSolution @19:11:33.53 delay 2 seconds @19:11:36.05 AS.reconfigure ASconfig @19:11:36.12 AS.seek 1ml @19:12:33.87 delay 1 second @19:12:34.89 AFV.select :holdingCoil @19:12:34.99 delay 1 second @19:12:36.02 AS.reconfigure ASfast @19:12:36.07 AS.seek 0ml @19:13:03.42 ARV.dial between carrier and air1 @19:13:03.92 ATV.dial :air3 @19:13:05.23 AS.reconfigure ASslow1 @19:13:05.29 AS.seek 0.01ml @19:13:07.67 delay 2 seconds @19:13:09.69 ATV.dial :bleach @19:13:10.31 AS.seek 0.81ml @19:15:51.47 delay 3 seconds @19:15:54.49 ATV.dial :air3 @19:15:55.06 delay 3 seconds @19:15:58.13 AS.seek 0.835ml @19:16:03.42 delay 3 seconds @19:16:06.44 ATV.dial :bleach @19:16:07.06 AS.seek 0.82ml @19:16:10.37 delay 3 seconds @19:16:13.39 ATV.dial PCR @19:16:13.79 AS.reconfigure ASconfig @19:16:13.85 AS.seek :empty @19:17:01.32 PCR.program :bleachProfile @19:17:01.38 PCR program should complete in < 2:45 @19:17:01.39 PCR.run 10:00 @19:20:00.20 PCR.off @19:20:00.23 AFV.select :carrierSolution @19:20:00.35 delay 2 seconds @19:20:02.38 AS.seek 1ml @19:21:00.22 delay 1 second @19:21:01.24 AFV.select :holdingCoil @19:21:01.34 delay 1 second @19:21:02.37 AS.reconfigure ASfast @19:21:02.42 AS.seek 0ml @19:21:29.79 delay 3 seconds @19:21:32.81 AFV.select :carrierSolution @19:21:32.91 delay 2 seconds @19:21:34.94 AS.reconfigure ASconfig @19:21:35.00 AS.seek 1ml @19:22:32.75 delay 1 second @19:22:33.77 AFV.select :holdingCoil @19:22:33.87 delay 1 second @19:22:34.90 AS.reconfigure ASfast @19:22:34.95 AS.seek 0ml @19:23:02.31 delay 3 seconds @19:23:05.33 AFV.select :carrierSolution @19:23:05.43 delay 2 seconds @19:23:07.46 AS.reconfigure ASconfig @19:23:07.51 AS.seek 1ml @19:24:05.26 delay 1 second @19:24:06.28 AFV.select :holdingCoil @19:24:06.38 delay 1 second @19:24:07.41 AS.reconfigure ASfast @19:24:07.46 AS.seek 0ml @19:24:34.83 delay 3 seconds @19:24:37.85 AFV.select :carrierSolution @19:24:37.95 delay 2 seconds @19:24:39.98 AS.reconfigure ASconfig @19:24:40.04 AS.seek 1ml @19:25:37.79 delay 1 second @19:25:38.81 AFV.select :holdingCoil @19:25:38.91 delay 1 second @19:25:39.94 AS.reconfigure ASfast @19:25:39.99 AS.seek 0ml @19:26:07.37 delay 3 seconds @19:26:10.39 AFV.select :carrierSolution @19:26:10.49 delay 2 seconds @19:26:12.52 AS.reconfigure ASconfig @19:26:12.58 AS.seek 1ml @19:27:10.34 delay 1 second @19:27:11.36 AFV.select :holdingCoil @19:27:11.46 delay 1 second @19:27:12.49 AS.reconfigure ASnorm @19:27:12.54 AS.seek 0ml @19:28:03.28 Setting up PCR for Ss -- writing /var/log/esp/Ss18nov2119h150ml.pcr @19:28:03.29 Building PCR Slug from ASV with MM1 mix, PP2 primer @19:28:03.32 Priming with carrier @19:28:03.34 ATV.dial ARV @19:28:03.97 ARV.dial :carrier @19:28:04.31 AS.seek 0.5ml @19:28:29.89 AFV.select :carrierSolution @19:28:30.00 delay 2 seconds @19:28:32.03 AS.reconfigure ASconfig @19:28:32.09 AS.seek 1ml @19:29:01.14 delay 1 second @19:29:02.16 AFV.select :holdingCoil @19:29:02.26 delay 1 second @19:29:03.28 ATV.dial :waste @19:29:04.95 AS.reconfigure ASnorm @19:29:05.02 AS.seek :empty @19:29:55.78 AFV.select :carrierSolution @19:29:55.89 delay 2 seconds @19:29:57.92 AS.reconfigure ASconfig @19:29:57.98 AS.seek 0.5ml @19:30:27.11 delay 1 second @19:30:28.64 AFV.select :holdingCoil @19:30:28.74 Building Slug @19:30:28.76 ATV.dial :ARV @19:30:30.51 delay 2 seconds @19:30:32.53 ARV.dial :air1 @19:30:33.03 delay 2 seconds @19:30:35.10 AS.reconfigure ASslow2 @19:30:35.16 AS.seek 0.506ml @19:30:41.67 delay 2 seconds @19:30:43.69 ARV.dial :MM1 @19:30:44.31 delay 2 seconds @19:30:46.33 Adding MM1 @19:30:46.42 AS.seek 0.524ml @19:31:04.73 delay 25 seconds @19:31:29.75 ARV.dial :PP2 @19:31:30.15 Adding PP2 @19:31:30.16 delay 2 seconds @19:31:32.24 AS.seek 0.53ml @19:31:38.43 delay 10 seconds @19:31:48.46 ARV.dial :air2 @19:31:48.86 adding ARV air @19:31:48.87 delay 2 seconds @19:31:50.95 AS.seek 0.536ml @19:31:57.25 delay 5 seconds @19:32:02.27 ARV.dial :carrier @19:32:03.58 delay 7 seconds @19:32:10.66 AS.seek 0.594ml @19:33:09.42 delay 5 seconds @19:33:14.44 ATV.dial :ASV @19:33:14.86 delay 2 seconds @19:33:16.94 AS.seek 0.6ml @19:33:23.15 delay 4 seconds @19:33:27.17 ATV.dial ARV @19:33:27.73 delay 2 seconds @19:33:29.75 ARV.dial :carrier @19:33:29.76 delay 2 seconds @19:33:31.84 AS.seek 0.65ml @19:34:22.37 delay 4 seconds @19:34:26.39 ATV.dial PCR @19:34:27.11 ASV.dial :waste1 @19:34:27.12 ARV.dial :air1 @19:34:27.55 Positioning Slug by 0.324ml @19:34:27.65 AS.seek 0.326ml @19:39:53.58 delay 5 seconds @19:39:58.60 ATV.dial ARV @19:39:59.37 ASV.dial :PCRlock @19:39:59.78 delay 5 seconds @19:40:04.80 Running Ss Profile60C350 thermocycling @19:40:04.82 PCR.program Profile60C350 @19:40:04.97 Profile60C350 should complete in < 1:48:05 @19:40:04.98 PCR.run 2:20:06.25 @21:02:26.64 PCR.off @21:02:28.64 PCR for Ss completed -- closed /var/log/esp/Ss18nov2119h150ml.pcr @21:02:28.71 Cleaning PCR @21:02:28.72 AFV.select :carrierSolution @21:02:28.84 delay 2 seconds @21:02:30.87 AS.reconfigure ASconfig @21:02:30.92 AS.seek 0.5ml @21:02:41.21 delay 1 second @21:02:42.23 AFV.select :holdingCoil @21:02:42.33 ASV.dial :waste1 @21:02:42.74 ATV.dial :air3 @21:02:44.03 AS.reconfigure ASslow1 @21:02:44.09 AS.seek 0.51ml @21:02:46.38 delay 2 seconds @21:02:48.40 ATV.dial :bleach @21:02:49.02 AS.seek 0.81ml @21:03:49.63 delay 3 seconds @21:03:52.65 ATV.dial :air3 @21:03:53.21 delay 3 seconds @21:03:56.28 AS.seek 0.835ml @21:04:01.61 delay 3 seconds @21:04:04.63 ATV.dial :bleach @21:04:05.25 AS.seek 0.82ml @21:04:08.58 delay 3 seconds @21:04:11.60 ARV.dial :waste @21:04:12.24 delay 3 seconds @21:04:15.26 ATV.dial :ARV @21:04:16.25 AS.reconfigure ASconfig @21:04:16.31 AS.seek 0.47ml @21:04:36.72 AFV.select :carrierSolution @21:04:36.83 delay 2 seconds @21:04:38.86 AS.seek 1ml @21:05:09.60 delay 1 second @21:05:10.62 AFV.select :holdingCoil @21:05:10.72 delay 1 second @21:05:11.75 AS.reconfigure ASfast @21:05:11.81 AS.seek 0ml @21:05:39.16 AFV.select :carrierSolution @21:05:39.27 delay 2 seconds @21:05:41.30 AS.reconfigure ASconfig @21:05:41.36 AS.seek 1ml @21:06:39.14 delay 1 second @21:06:40.16 AFV.select :holdingCoil @21:06:40.26 delay 1 second @21:06:41.29 AS.reconfigure ASfast @21:06:41.34 AS.seek 0ml @21:07:09.19 AFV.select :carrierSolution @21:07:09.30 delay 2 seconds @21:07:11.33 AS.reconfigure ASconfig @21:07:11.38 AS.seek 1ml @21:08:09.16 delay 1 second @21:08:10.18 AFV.select :holdingCoil @21:08:10.28 delay 1 second @21:08:11.31 AS.reconfigure ASfast @21:08:11.36 AS.seek 0ml @21:08:38.71 ARV.dial between carrier and air1 @21:08:39.24 ATV.dial :air3 @21:08:40.53 AS.reconfigure ASslow1 @21:08:40.59 AS.seek 0.01ml @21:08:42.94 delay 2 seconds @21:08:44.96 ATV.dial :bleach @21:08:45.58 AS.seek 0.81ml @21:11:26.72 delay 3 seconds @21:11:29.74 ATV.dial :air3 @21:11:30.30 delay 3 seconds @21:11:33.37 AS.seek 0.835ml @21:11:39.54 delay 3 seconds @21:11:42.56 ATV.dial :bleach @21:11:43.19 AS.seek 0.82ml @21:11:46.54 delay 3 seconds @21:11:49.56 ATV.dial PCR @21:11:49.97 AS.reconfigure ASconfig @21:11:50.03 AS.seek :empty @21:12:37.52 PCR.program :bleachProfile @21:12:37.57 PCR program should complete in < 2:45 @21:12:37.58 PCR.run 10:00 @21:15:36.38 PCR.off @21:15:36.42 AFV.select :carrierSolution @21:15:36.53 delay 2 seconds @21:15:38.56 AS.seek 1ml @21:16:36.40 delay 1 second @21:16:37.42 AFV.select :holdingCoil @21:16:37.52 delay 1 second @21:16:38.55 AS.reconfigure ASfast @21:16:38.60 AS.seek 0ml @21:17:05.97 delay 3 seconds @21:17:08.99 AFV.select :carrierSolution @21:17:09.09 delay 2 seconds @21:17:11.12 AS.reconfigure ASconfig @21:17:11.18 AS.seek 1ml @21:18:08.93 delay 1 second @21:18:09.95 AFV.select :holdingCoil @21:18:10.05 delay 1 second @21:18:11.08 AS.reconfigure ASfast @21:18:11.13 AS.seek 0ml @21:18:38.47 delay 3 seconds @21:18:41.49 AFV.select :carrierSolution @21:18:41.59 delay 2 seconds @21:18:43.62 AS.reconfigure ASconfig @21:18:43.68 AS.seek 1ml @21:19:41.46 delay 1 second @21:19:42.48 AFV.select :holdingCoil @21:19:42.58 delay 1 second @21:19:43.61 AS.reconfigure ASfast @21:19:43.66 AS.seek 0ml @21:20:11.02 delay 3 seconds @21:20:14.04 AFV.select :carrierSolution @21:20:14.14 delay 2 seconds @21:20:16.17 AS.reconfigure ASconfig @21:20:16.23 AS.seek 1ml @21:21:14.00 delay 1 second @21:21:15.02 AFV.select :holdingCoil @21:21:15.12 delay 1 second @21:21:16.15 AS.reconfigure ASfast @21:21:16.20 AS.seek 0ml @21:21:43.57 delay 3 seconds @21:21:46.59 AFV.select :carrierSolution @21:21:46.69 delay 2 seconds @21:21:48.72 AS.reconfigure ASconfig @21:21:48.78 AS.seek 1ml @21:22:46.54 delay 1 second @21:22:47.56 AFV.select :holdingCoil @21:22:47.66 delay 1 second @21:22:48.69 AS.reconfigure ASnorm @21:22:48.74 AS.seek 0ml @21:23:39.46 Setting up PCR for IPc -- writing /var/log/esp/IPc18nov2121h150ml.pcr @21:23:39.47 Building PCR Slug from ASV with MM1 mix, PP1 primer @21:23:39.50 Priming with carrier @21:23:39.52 ATV.dial ARV @21:23:40.24 ARV.dial :carrier @21:23:40.60 AS.seek 0.5ml @21:24:06.15 AFV.select :carrierSolution @21:24:06.26 delay 2 seconds @21:24:08.29 AS.reconfigure ASconfig @21:24:08.35 AS.seek 1ml @21:24:37.38 delay 1 second @21:24:38.40 AFV.select :holdingCoil @21:24:38.50 delay 1 second @21:24:39.52 ATV.dial :waste @21:24:41.18 AS.reconfigure ASnorm @21:24:41.24 AS.seek :empty @21:25:32.01 AFV.select :carrierSolution @21:25:32.12 delay 2 seconds @21:25:34.15 AS.reconfigure ASconfig @21:25:34.71 AS.seek 0.5ml @21:26:03.85 delay 1 second @21:26:04.87 AFV.select :holdingCoil @21:26:04.97 Building Slug @21:26:04.99 ATV.dial :ARV @21:26:06.65 delay 2 seconds @21:26:08.67 ARV.dial :air1 @21:26:09.08 delay 2 seconds @21:26:11.15 AS.reconfigure ASslow2 @21:26:11.22 AS.seek 0.506ml @21:26:17.68 delay 2 seconds @21:26:19.70 ARV.dial :MM1 @21:26:20.30 delay 2 seconds @21:26:22.32 Adding MM1 @21:26:22.41 AS.seek 0.524ml @21:26:40.72 delay 25 seconds @21:27:05.74 ARV.dial :PP1 @21:27:06.15 Adding PP1 @21:27:06.16 delay 2 seconds @21:27:08.24 AS.seek 0.53ml @21:27:14.52 delay 10 seconds @21:27:24.55 ARV.dial :air1 @21:27:24.96 adding ARV air @21:27:24.97 delay 2 seconds @21:27:27.05 AS.seek 0.536ml @21:27:33.62 delay 5 seconds @21:27:38.64 ARV.dial :carrier @21:27:39.05 delay 7 seconds @21:27:46.13 AS.seek 0.594ml @21:28:44.69 delay 5 seconds @21:28:49.71 ATV.dial :ASV @21:28:50.10 delay 2 seconds @21:28:52.17 AS.seek 0.6ml @21:28:58.46 delay 4 seconds @21:29:02.48 ATV.dial ARV @21:29:03.13 delay 2 seconds @21:29:05.15 ARV.dial :carrier @21:29:05.16 delay 2 seconds @21:29:07.24 AS.seek 0.65ml @21:29:57.83 delay 4 seconds @21:30:01.85 ATV.dial PCR @21:30:02.47 ASV.dial :waste1 @21:30:02.48 ARV.dial :air1 @21:30:02.91 Positioning Slug by 0.324ml @21:30:03.00 AS.seek 0.326ml @21:35:29.07 delay 5 seconds @21:35:34.09 ATV.dial ARV @21:35:34.88 ASV.dial :PCRlock @21:35:35.29 delay 5 seconds @21:35:40.31 Running IPc ProfileIPc thermocycling @21:35:40.33 PCR.program ProfileIPc @21:35:40.48 ProfileIPc should complete in < 1:50:49 @21:35:40.49 PCR.run 2:23:31.25 @22:59:44.95 PCR.off @22:59:47.46 PCR for IPc completed -- closed /var/log/esp/IPc18nov2121h150ml.pcr @22:59:47.48 Full MFB Decontamination @22:59:47.50 Decon extraction column and decon AIV @22:59:47.53 SPE.hold 2:46:40,55C @22:59:47.59 ASV.dial :SPEdelivery @22:59:48.25 delay 20 seconds @23:00:08.28 AFV.select :carrierSolution @23:00:08.38 delay 2 seconds @23:00:10.41 AS.reconfigure ASconfig @23:00:10.46 AS.seek 0.5ml @23:00:20.76 delay 1 second @23:00:21.78 AFV.select :holdingCoil @23:00:21.88 ATV.dial :air1 @23:00:23.82 AS.reconfigure ASslow1 @23:00:23.88 AS.seek 0.52ml @23:00:28.17 ATV.dial :ASV @23:00:29.97 ASV.dial :SPErecovery @23:00:30.65 AS.seek 0.62ml @23:00:51.50 ATV.dial :waste @23:00:53.54 AS.reconfigure ASnorm @23:00:53.60 AS.seek :empty @23:01:25.23 AFV.select :holdingCoil @23:01:25.24 ATV.dial :waste @23:01:25.27 AS.reconfigure ASconfig @23:01:25.34 AS.seek 0ml @23:01:25.64 AFV.select :carrierSolution @23:01:25.75 delay 2 seconds @23:01:27.78 AS.seek 0.5ml @23:01:56.81 delay 1 second @23:01:57.83 AFV.select :holdingCoil @23:01:57.93 ATV.dial :air3 @23:01:58.65 AS.reconfigure ASslow1 @23:01:58.71 AS.seek 0.52ml @23:02:03.04 ATV.dial :air3 @23:02:03.13 AS.seek 0.53ml @23:02:05.34 delay 2 seconds @23:02:07.36 ATV.dial :bleach @23:02:07.84 AS.seek 0.71ml @23:02:44.31 delay 3 seconds @23:02:47.33 ATV.dial :air3 @23:02:47.92 delay 3 seconds @23:02:50.99 AS.seek 0.735ml @23:02:56.30 delay 3 seconds @23:02:59.32 ATV.dial :bleach @23:02:59.98 AS.seek 0.72ml @23:03:03.26 delay 3 seconds @23:03:06.28 ATV.dial ASV @23:03:07.37 ASV.dial :SPErecovery @23:03:07.46 AS.seek 0.365ml @23:04:19.11 ATV.dial :waste @23:04:20.86 AS.reconfigure ASnorm @23:04:20.92 AS.seek :empty @23:04:39.72 ATV.dial :air3 @23:04:40.42 AS.reconfigure ASslow1 @23:04:40.48 AS.seek 0.009ml @23:04:42.84 delay 2 seconds @23:04:44.86 ATV.dial :bleach @23:04:45.34 AS.seek 0.579ml @23:06:40.20 delay 3 seconds @23:06:43.22 ATV.dial :air3 @23:06:43.79 delay 3 seconds @23:06:46.86 AS.seek 0.604ml @23:06:52.19 delay 3 seconds @23:06:55.21 ATV.dial :bleach @23:06:55.85 AS.seek 0.589ml @23:06:59.17 delay 3 seconds @23:07:02.19 ATV.dial :ASV @23:07:03.23 ASV.dial :wasteR @23:07:03.63 AS.seek 0ml @23:09:02.37 delay 1:00 @23:10:02.40 ATV.dial ASV @23:10:02.41 AFV.select :carrierSolution @23:10:02.53 delay 2 seconds @23:10:04.56 AS.reconfigure ASconfig @23:10:04.61 AS.seek 1ml @23:11:02.39 delay 1 second @23:11:03.41 AFV.select :holdingCoil @23:11:03.51 delay 1 second @23:11:04.53 ASV.dial :wasteR @23:11:04.55 AS.reconfigure ASslow1 @23:11:04.62 AS.seek 0ml @23:14:26.00 delay 10 seconds @23:14:36.03 ATV.dial :air3 @23:14:37.56 AS.seek 0.01ml @23:14:39.89 delay 2 seconds @23:14:41.91 ATV.dial :bleach @23:14:42.56 AS.seek 0.58ml @23:16:37.47 delay 3 seconds @23:16:40.49 ATV.dial :air3 @23:16:41.06 delay 3 seconds @23:16:44.13 AS.seek 0.605ml @23:16:49.44 delay 3 seconds @23:16:52.46 ATV.dial :bleach @23:16:53.10 AS.seek 0.59ml @23:16:56.39 delay 3 seconds @23:16:59.41 ATV.dial ASV @23:17:00.47 ASV.dial :SPEdelivery @23:17:00.85 AS.seek 0ml @23:18:59.79 delay 1:00 @23:19:59.82 AFV.select :carrierSolution @23:19:59.92 delay 2 seconds @23:20:01.95 AS.reconfigure ASconfig @23:20:02.01 AS.seek 1ml @23:21:00.29 delay 1 second @23:21:01.31 AFV.select :holdingCoil @23:21:01.41 delay 1 second @23:21:02.43 ATV.dial ASV @23:21:02.44 ASV.dial :SPEdelivery @23:21:02.47 AS.reconfigure ASslow1 @23:21:02.54 AS.seek 0ml @23:24:23.94 delay 10 seconds @23:24:33.97 ATV.dial :air2 @23:24:34.81 AS.reconfigure ASslow0 @23:24:34.88 AS.seek 0.8ml @23:25:55.65 delay 3 seconds @23:25:58.67 ATV.dial :ASV @23:25:59.67 ASV.dial :SPEdelivery @23:25:59.69 AS.reconfigure ASslow1 @23:25:59.76 AS.seek 0.03ml @23:28:34.90 delay 20 seconds @23:28:54.93 ATV.dial :waste,via: :air3 @23:28:56.67 AS.reconfigure ASconfig @23:28:56.73 AS.seek :empty @23:28:58.81 SPE.coast @23:28:58.89 Removing Bleach from Mixing Coil @23:28:58.90 AFV.select :carrierSolution @23:28:59.02 delay 2 seconds @23:29:01.05 AS.seek 0.5ml @23:29:30.15 delay 1 second @23:29:31.17 AFV.select :holdingCoil @23:29:31.27 ATV.dial :ASV @23:29:32.97 ASV.dial :SPErecovery @23:29:33.61 AS.reconfigure ASslow1 @23:29:33.67 AS.seek 0.73ml @23:30:20.20 ASV.dial :wasteD @23:30:20.80 AS.reconfigure ASnorm @23:30:20.86 AS.seek 0ml @23:30:58.00 AFV.select :carrierSolution @23:30:58.11 delay 2 seconds @23:31:00.14 AS.reconfigure ASconfig @23:31:00.19 AS.seek 0.5ml @23:31:29.28 delay 1 second @23:31:30.30 AFV.select :holdingCoil @23:31:30.40 ATV.dial ASV @23:31:30.42 ASV.dial :SPErecovery @23:31:31.07 AS.reconfigure ASslow0 @23:31:31.13 AS.seek 0.3ml @23:31:51.59 ATV.dial :air2 @23:31:52.64 AS.reconfigure ASslow1 @23:31:52.70 AS.seek 0.32ml @23:31:57.02 ATV.dial ASV @23:31:58.06 AS.reconfigure ASslow0 @23:31:58.12 AS.seek 0.57ml @23:32:23.51 ASV.dial :wasteD @23:32:24.11 AS.reconfigure ASnorm @23:32:24.17 AS.seek 0ml @23:32:53.23 AFV.select :carrierSolution @23:32:53.34 delay 2 seconds @23:32:55.37 AS.reconfigure ASconfig @23:32:55.43 AS.seek 0.5ml @23:33:24.50 delay 1 second @23:33:25.52 AFV.select :holdingCoil @23:33:25.62 ATV.dial ASV @23:33:25.63 ASV.dial :SPErecovery @23:33:26.29 AS.reconfigure ASslow0 @23:33:26.35 AS.seek 0.3ml @23:33:46.80 ATV.dial :air2 @23:33:47.84 AS.reconfigure ASslow1 @23:33:47.90 AS.seek 0.32ml @23:33:52.26 ATV.dial ASV @23:33:53.31 AS.reconfigure ASslow0 @23:33:53.37 AS.seek 0.57ml @23:34:18.83 ASV.dial :wasteD @23:34:19.50 AS.reconfigure ASnorm @23:34:19.56 AS.seek 0ml @23:34:48.64 ATV.dial :air2 @23:34:49.62 AS.reconfigure ASslow0 @23:34:49.68 AS.seek 0.3ml @23:35:20.14 ATV.dial :ASV @23:35:21.12 ASV.dial :wasteD @23:35:21.14 AS.reconfigure ASconfig @23:35:21.21 AS.seek 0.05ml @23:35:35.89 ATV.dial :waste @23:35:37.76 AS.seek 0ml @23:35:40.98 ASV.dial :SPEdelivery @23:35:41.98 deconLysateINT @23:35:41.99 ATV.dial :air3 @23:35:42.70 AS.reconfigure ASslow1 @23:35:42.76 AS.seek 0.01ml @23:35:45.09 delay 2 seconds @23:35:47.11 ATV.dial :bleach @23:35:48.08 AS.seek 0.96ml @23:38:59.41 delay 3 seconds @23:39:02.43 ATV.dial :air3 @23:39:02.98 delay 3 seconds @23:39:06.05 AS.seek 0.985ml @23:39:11.37 delay 3 seconds @23:39:14.39 ATV.dial :bleach @23:39:15.01 AS.seek 0.97ml @23:39:18.31 delay 3 seconds @23:39:21.33 ATV.dial AIV @23:39:22.69 AIV.dial :coilToMFB @23:39:22.70 AWV.select :waste @23:39:22.72 AFV.select :carrierSolution @23:39:22.85 delay 2 seconds @23:39:24.88 AS.reconfigure ASconfig @23:39:24.94 AS.seek 1ml @23:39:26.98 delay 1 second @23:39:28.00 AFV.select :holdingCoil @23:39:28.10 delay 1 second @23:39:29.13 AS.reconfigure ASslow0 @23:39:29.18 AS.seek :empty @23:41:10.16 AFV.select :carrierSolution @23:41:10.28 delay 2 seconds @23:41:12.31 AS.reconfigure ASconfig @23:41:12.37 AS.seek 1ml @23:42:10.19 delay 1 second @23:42:11.21 AFV.select :holdingCoil @23:42:11.31 delay 1 second @23:42:12.34 AS.reconfigure ASslow0 @23:42:12.39 AS.seek :empty @23:43:53.39 AFV.select :carrierSolution @23:43:53.50 delay 2 seconds @23:43:55.53 AS.reconfigure ASconfig @23:43:55.59 AS.seek 1ml @23:44:53.39 delay 1 second @23:44:54.41 AFV.select :holdingCoil @23:44:54.51 delay 1 second @23:44:55.54 AS.reconfigure ASslow0 @23:44:55.59 AS.seek :empty @23:46:36.55 ATV.dial :air2 @23:46:37.22 AS.reconfigure ASslow1 @23:46:37.28 AS.seek 0.009ml @23:46:39.59 AS.reconfigure ASslow0 @23:46:39.65 AS.seek 1ml @23:48:19.61 ATV.dial AIV @23:48:20.38 AS.seek 0.02ml @23:49:59.27 ATV.dial :waste @23:50:00.93 AS.seek 0ml @23:50:03.27 AWV.select :air @23:50:03.43 Cleaning PCR @23:50:03.44 AFV.select :carrierSolution @23:50:03.56 delay 2 seconds @23:50:05.59 AS.reconfigure ASconfig @23:50:05.64 AS.seek 0.5ml @23:50:34.69 delay 1 second @23:50:35.71 AFV.select :holdingCoil @23:50:35.81 ASV.dial :waste1 @23:50:36.19 ATV.dial :air3 @23:50:36.87 AS.reconfigure ASslow1 @23:50:36.93 AS.seek 0.51ml @23:50:39.23 delay 2 seconds @23:50:41.25 ATV.dial :bleach @23:50:41.70 AS.seek 0.81ml @23:51:42.28 delay 3 seconds @23:51:45.30 ATV.dial :air3 @23:51:45.86 delay 3 seconds @23:51:48.93 AS.seek 0.835ml @23:51:54.24 delay 3 seconds @23:51:57.26 ATV.dial :bleach @23:51:57.89 AS.seek 0.82ml @23:52:01.16 delay 3 seconds @23:52:04.18 ARV.dial :waste @23:52:04.81 delay 3 seconds @23:52:07.83 ATV.dial :ARV @23:52:08.70 AS.reconfigure ASconfig @23:52:08.76 AS.seek 0.47ml @23:52:29.16 AFV.select :carrierSolution @23:52:29.27 delay 2 seconds @23:52:31.30 AS.seek 1ml @23:53:02.08 delay 1 second @23:53:03.10 AFV.select :holdingCoil @23:53:03.20 delay 1 second @23:53:04.23 AS.reconfigure ASfast @23:53:04.28 AS.seek 0ml @23:53:31.66 AFV.select :carrierSolution @23:53:31.77 delay 2 seconds @23:53:33.80 AS.reconfigure ASconfig @23:53:33.85 AS.seek 1ml @23:54:31.64 delay 1 second @23:54:32.66 AFV.select :holdingCoil @23:54:32.76 delay 1 second @23:54:33.79 AS.reconfigure ASfast @23:54:33.84 AS.seek 0ml @23:55:01.20 AFV.select :carrierSolution @23:55:01.31 delay 2 seconds @23:55:03.34 AS.reconfigure ASconfig @23:55:03.39 AS.seek 1ml @23:56:01.19 delay 1 second @23:56:02.21 AFV.select :holdingCoil @23:56:02.31 delay 1 second @23:56:03.34 AS.reconfigure ASfast @23:56:03.91 AS.seek 0ml @23:56:31.30 ARV.dial between carrier and air1 @23:56:31.83 ATV.dial :air3 @23:56:33.12 AS.reconfigure ASslow1 @23:56:33.18 AS.seek 0.01ml @23:56:35.52 delay 2 seconds @23:56:37.54 ATV.dial :bleach @23:56:38.15 AS.seek 0.81ml @23:59:19.30 delay 3 seconds @23:59:22.32 ATV.dial :air3 @23:59:22.89 delay 3 seconds @23:59:25.96 AS.seek 0.835ml @23:59:31.28 delay 3 seconds @23:59:34.30 ATV.dial :bleach @23:59:34.92 AS.seek 0.82ml @23:59:38.22 delay 3 seconds @23:59:41.24 ATV.dial PCR @23:59:41.64 AS.reconfigure ASconfig @23:59:41.70 AS.seek :empty @00:00:29.17CET22-Nov-18 PCR.program :bleachProfile @00:00:29.23 PCR program should complete in < 2:45 @00:00:29.24 PCR.run 10:00 @00:03:31.07 PCR.off @00:03:31.11 AFV.select :carrierSolution @00:03:31.22 delay 2 seconds @00:03:33.25 AS.seek 1ml @00:04:31.09 delay 1 second @00:04:32.11 AFV.select :holdingCoil @00:04:32.21 delay 1 second @00:04:33.24 AS.reconfigure ASfast @00:04:33.29 AS.seek 0ml @00:05:00.64 delay 3 seconds @00:05:03.66 AFV.select :carrierSolution @00:05:03.76 delay 2 seconds @00:05:05.79 AS.reconfigure ASconfig @00:05:05.85 AS.seek 1ml @00:06:03.61 delay 1 second @00:06:04.63 AFV.select :holdingCoil @00:06:04.73 delay 1 second @00:06:05.76 AS.reconfigure ASfast @00:06:05.81 AS.seek 0ml @00:06:33.20 delay 3 seconds @00:06:36.22 AFV.select :carrierSolution @00:06:36.32 delay 2 seconds @00:06:38.35 AS.reconfigure ASconfig @00:06:38.41 AS.seek 1ml @00:07:36.20 delay 1 second @00:07:37.22 AFV.select :holdingCoil @00:07:37.32 delay 1 second @00:07:38.35 AS.reconfigure ASfast @00:07:38.40 AS.seek 0ml @00:08:05.75 delay 3 seconds @00:08:08.77 AFV.select :carrierSolution @00:08:08.87 delay 2 seconds @00:08:10.90 AS.reconfigure ASconfig @00:08:10.96 AS.seek 1ml @00:09:08.77 delay 1 second @00:09:09.79 AFV.select :holdingCoil @00:09:09.89 delay 1 second @00:09:10.92 AS.reconfigure ASfast @00:09:10.97 AS.seek 0ml @00:09:38.33 delay 3 seconds @00:09:41.35 AFV.select :carrierSolution @00:09:41.45 delay 2 seconds @00:09:43.48 AS.reconfigure ASconfig @00:09:43.53 AS.seek 1ml @00:10:41.38 delay 1 second @00:10:42.40 AFV.select :holdingCoil @00:10:42.50 delay 1 second @00:10:43.53 AS.reconfigure ASnorm @00:10:43.58 AS.seek 0ml @00:11:34.31 AFV.select :carrierSolution @00:11:34.42 delay 2 seconds @00:11:36.45 AS.reconfigure ASconfig @00:11:36.50 AS.seek 1ml @00:12:34.28 delay 1 second @00:12:35.30 AFV.select :holdingCoil @00:12:35.40 delay 1 second @00:12:36.42 ATV.dial :waste @00:12:37.66 AS.reconfigure ASnorm @00:12:37.72 AS.seek 0.5ml @00:13:03.28 ATV.dial :waste2 @00:13:04.57 AS.seek 0ml @00:13:30.16
Asleep after completing 18tun_postDeploy2 assays @00:13:30.17 GoodNews.email "{3 => 3, 4 => 9}\nCan@00:05:36, 19.7C, 38% humidity, 14.8psia, 12.046V, 0.468A, 0.462A avg, 5.64W",Subject: "Asleep after completing 18tun_postDeploy2 assays" @00:13:30.20
Mission runtime was 12:17:55.405844 @00:13:37.84 Session Ended @16:24:08.40CET24-Nov-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @16:24:10.12 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @16:24:10.37 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @16:24:11.86 LoadError in MAIN -- no such file to load -- /home/esp/esp2/utils/sleep.rb @16:24:11.94 Loading /home/esp/esp2/mission/18tun_postDeploy3.rb mission @16:24:12.00 NoMethodError in MAIN -- undefined method `minRate' for nil:NilClass @16:24:12.01 Waiting to Resume from Checkpoint "Loading /home/esp/esp2/mission/18tun_postDeploy3.rb mission" @16:24:27.87 Session Begun @16:25:49.55 -> showlog 10 @16:26:37.02 -> abort! 'try again' @16:26:37.03 CmdServer::Client::Error in mwj -- Aborting Mission: try again @16:26:37.05 NoMethodError in mwj -- undefined method `abort!' for Kernel:Module @16:26:50.66 -> abort! "try again" @16:26:50.67 CmdServer::Client::Error in mwj -- Aborting Mission: try again @16:26:50.70 NoMethodError in mwj -- undefined method `abort!' for Kernel:Module @16:27:00.83 -> Abort! "try again" @16:27:00.84 NoMethodError in mwj -- undefined method `Abort!' for # @16:31:26.08CET24-Nov-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @16:31:27.81 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @16:31:28.07 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @16:31:32.40 Polling Can every 10:00 @16:31:34.16 SPE.coast @16:31:34.21 SPE.configure SPEconfig @16:31:34.29 AS.configure ASconfig @16:31:34.38 SS.reconfigure SSconfig @16:31:34.42 SS.hold @16:31:34.48 SS.configure SSconfig @16:31:34.56 CC.reconfigure CCconfig @16:31:34.60 CC.hold @16:31:34.64 CC.configure CCconfig @16:31:34.72 CS.reconfigure CSconfig @16:31:34.76 CS.hold @16:31:34.84 CS.configure CSconfig @16:31:34.92 PC.reconfigure PCconfig @16:31:34.96 PC.hold @16:31:35.35 PC.configure PCconfig @16:31:35.43 PS.configure PSconfig @16:31:35.53 Forearm.reconfigure LooseForearm @16:31:35.57 Forearm.hold @16:31:35.61 Forearm.configure LooseForearm @16:31:35.69 Elbow.reconfigure LooseElbow @16:31:35.73 Elbow.hold @16:31:35.77 Elbow.configure LooseElbow @16:31:35.87 SC.reconfigure SCloose @16:31:35.91 SC.hold @16:31:35.96 SC.configure SCloose @16:31:36.04 SE.reconfigure SEconfig @16:31:36.08 SE.hold @16:31:36.13 SE.configure SEconfig @16:31:36.17 CH.coast @16:31:36.21 CH.configure CHconfig @16:31:36.24 PH.coast @16:31:36.29 PH.configure PHconfig @16:31:36.32 PCR.off @16:31:36.35 PCR.pidtemp= 50,0.1,0,300,25,25 @16:31:36.37 PCR.caltemp= 2.7073,0.0373,0 @16:31:36.40 All dwarf controllers running firmware version 3.75 @16:31:36.54 Loading /home/esp/esp2/mission/18tun_postDeploy3.rb mission @16:31:36.60 Preparing to sleep until +0:00:15 @16:31:36.62 GoodNews.email "Starting 18tun_postDeploy3 w/pucks in tube 4\n{}\nCan@16:31:32, 19.5C, 38% humidity, 14.6psia, 12.046V, 0.455A, 0.455A avg, 5.49W",Subject: "18tun_postDeploy3 begun" @16:31:36.70 SS.coast @16:31:36.72
Asleep til +0:00:15 @16:31:36.75 Continuing at 16:31 @16:31:36.77 CC.coast @16:31:36.86 CS.coast @16:31:36.89 PC.coast @16:31:36.93 Forearm.coast @16:31:36.96 Elbow.coast @16:31:36.99 SC.coast @16:31:37.02 SE.coast @16:31:37.40 Gate.power :core,:OFF @16:31:37.43 Gate.power :raw,:OFF @16:31:37.44 Shutdown @16:31:41.46 Session Begun @16:31:43.92 GoodNews.email "Then, in tube 4: mbaPhase\n/home/esp/esp2/mission/18tun_postDeploy3.rb:103\n{}\nCan@16:31:36, 19.6C, 38% humidity, 14.6psia, 12.046V, 0.455A, 0.455A avg, 5.49W",Subject: "Asleep until 16:31" @16:31:51.57
Powering core bus @16:31:56.48 Gate.power :raw,:ON @16:31:57.07 SPE.coast @16:31:57.14 SPE.configure SPEconfig @16:31:57.24 AS.configure ASconfig @16:31:57.37 SS.configure SSconfig @16:31:57.47 CC.configure CCconfig @16:31:57.57 CS.configure CSconfig @16:31:57.68 PC.configure PCconfig @16:31:57.78 PS.configure PSconfig @16:31:57.92 Forearm.configure LooseForearm @16:31:58.02 Elbow.configure LooseElbow @16:31:58.15 SC.configure SCloose @16:31:58.25 SE.configure SEconfig @16:31:58.30 CH.coast @16:31:58.38 CH.configure CHconfig @16:31:58.42 PH.coast @16:31:58.49 PH.configure PHconfig @16:31:58.54 PCR.off @16:31:58.57 PCR.pidtemp= 50,0.1,0,300,25,25 @16:31:58.63 PCR.caltemp= 2.7073,0.0373,0 @16:31:58.66 All dwarf controllers running firmware version 3.75 @16:31:58.78 Valve.reset @16:31:58.95 PCR.off @16:31:58.99 PCR.pidtemp= 50,0.1,0,300,25,25 @16:31:59.02 PCR.caltemp= 2.7073,0.0373,0 @16:31:59.12 Intake.select :close @16:31:59.20 Exhaust.select :close @16:31:59.24 CTV.dial :air @16:31:59.34 PSV.dial :air @16:31:59.47 CSV.dial :waste1 @16:31:59.50 PS.reconfigure PSconfig @16:31:59.52
Forearm.reconfigure LooseForearm @16:31:59.66 PS.home.seek 0ml @16:31:59.68
Forearm.home.seek :retract @16:31:59.78 SSV.dial :spit,avoiding: :exhaust @16:32:00.28 CS.reconfigure CSfast @16:32:00.31 SS.reconfigure SSconfig @16:32:00.43 CS.home.seek 0ml @16:32:00.47 SS.home.seek 0ml @16:32:00.59 Hand.close @16:32:00.62
Elbow.jog 1500 @16:32:01.10 Elbow.reconfigure LooseElbow @16:32:01.16 Elbow.home.seek :clear @16:32:05.80 SE.reconfigure SEconfig @16:32:05.86 SE.home.seek :down @16:32:06.54 SC.reconfigure SCsloppy @16:32:06.61 SC.home.seek :initial @16:32:08.91 PS.coast @16:32:10.57
Forearm.seek :retract @16:32:10.66 Elbow.seek :hammer @16:32:11.91 Forearm.reconfigure TightForearm @16:32:11.97 Forearm.seek 5 + 350 counts @16:32:13.05 Forearm.seek :retract @16:32:14.30 PC.reconfigure PCconfig @16:32:14.32
CC.reconfigure CCconfig @16:32:14.41 PC.home.seek :open @16:32:14.44
CC.home.seek :open @16:32:17.77 Phase [mbaPhase] @16:32:17.78 GoodNews.email "At 16:32:17 began in tube 4: mbaPhase\n/home/esp/esp2/mission/18tun_postDeploy3.rb:103\n{}\nCan@16:32:17, 19.5C, 38% humidity, 14.6psia, 11.972V, 0.733A, 0.727A avg, 8.77W",Subject: "Started 18tun_postDeploy3 phase mbaPhase" @16:32:17.82CET24-Nov-18
==================> STARTED MBAsuperphase (phase #1) <=== @16:32:17.93 Starting mfb @16:32:17.94 pcrBAC sampling at most 1200ml, wcr at most 1200ml @16:32:18.00 Loading sh1 puck from tube 4 into Collection Clamp @16:32:18.14 Hand.open @16:32:18.16 Readying the MFB @16:32:18.22 Homing MFB Syringe @16:32:18.23 AFV.select :holdingCoil @16:32:18.40 ATV.dial :air2 @16:32:19.08 AS.reconfigure ASconfig @16:32:19.17 AS.home.seek :home @16:32:19.29
SC.reconfigure SCtight @16:32:19.37 SC.seek 4 @16:32:23.20 SC.reconfigure SCloose @16:32:23.37 Elbow.reconfigure TightElbow @16:32:23.43 Elbow.seek 4 @16:32:23.93 Forearm.seek 4 + 700 counts @16:32:24.06 BACfans.email "Started mfb in tube 4 at 16:32:17CET24-Nov-18\n{}\nCan@16:32:17, 19.5C, 38% humidity, 14.6psia, 11.972V, 0.733A, 0.727A avg, 8.77W",Subject: "pcrBAC sampling at most 1200ml, wcr at most 1200ml" @16:32:24.75 AFV.select :carrierSolution @16:32:24.94 delay 2 seconds @16:32:25.00
SE.reconfigure SEpick @16:32:25.09 SE.seek beamBreak @16:32:26.98 AS.seek 1ml @16:32:27.58
SE.configure SEconfig @16:32:27.78 Elbow.seek 4 @16:32:28.00 Forearm.seek 4 + 300 counts @16:32:28.30 Forearm.reconfigure LooseForearm @16:32:28.36 Hand.close @16:32:29.11 SE.seek :down @16:32:29.34
Forearm.seek :retract @16:32:30.38 Elbow.seek CC @16:32:31.25 SE.coast @16:32:32.01
Elbow.reconfigure LooseElbow @16:32:32.13 Forearm.reconfigure TightForearm @16:32:32.22 Forearm.seek CC @16:32:33.92 CC.seek :closed @16:32:38.28 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.32 @16:32:38.29 Hand.open @16:32:39.15 Forearm.reconfigure LooseForearm @16:32:39.21 Forearm.seek :retract @16:32:40.20 SC.coast @16:32:40.25 collectSample 1200ml w/bubble point of 55psi @16:32:40.27 Priming 7ml @16:32:40.28 Intake.select :close @16:32:40.30 Exhaust.select :close @16:32:40.33 SS.hold @16:32:40.55 Calibrating pressure and temperature sensors @16:32:40.57 CTV.dial :air @16:32:40.60 SSV.dial :spit,avoiding: :exhaust @16:32:40.63 SS.reconfigure SSfast @16:32:40.71 SS.seek 5ml @16:32:43.56 SSV.dial CBV @16:32:43.59
CBV.dial SSV @16:32:45.14 delay 3.8 seconds @16:32:49.06 SSV.dial :spit @16:32:50.59 SS.seek 0ml @16:32:53.36 SSV.dial CBV @16:32:54.84 CTV.dial :intake @16:32:55.71 SSV.dial CBV @16:32:55.74
CBV.dial SSV @16:32:55.77 Allowing 40s for 7.0ml of remaining 7.0ml @16:32:55.79 Intake.select :open @16:32:57.98 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:32:58.00 SS.reconfigure SSinhale.with maxSpeed: 150 @16:32:58.07 SS.seek 7ml @16:33:04.05 Stroke completed -- waiting for pressure to equalize ... @16:33:18.00 Intake.select :close @16:33:18.15 SSV.dial :exhaust @16:33:19.02 Exhaust.select :open @16:33:19.14 SS.reconfigure SSexhale @16:33:19.21 SS.seek 0ml @16:33:19.89 delay 1 second @16:33:20.91 AFV.select :holdingCoil @16:33:21.02 delay 1 second @16:33:22.03 ATV.dial :waste @16:33:23.11 AS.reconfigure ASnorm @16:33:23.18 AS.home.seek :empty @16:33:23.24
Exhaust.select :close @16:33:23.37 Sampling 1200.0ml @16:33:23.38 Intake.select :close @16:33:23.40 Exhaust.select :close @16:33:23.42 SS.reconfigure SSconfig @16:33:23.50 SS.hold @16:33:23.70 CTV.dial :intake @16:33:23.72 SSV.dial CBV @16:33:23.75
CBV.dial SSV @16:33:24.66 Allowing 130s for 25.0ml of remaining 1200.0ml @16:33:24.67 Intake.select :open @16:33:26.87 Intake @15.9psia, vacuum from 9.4 to 13.4psi @16:33:26.89 SS.reconfigure SSinhale.with maxSpeed: 150 @16:33:26.96 SS.seek 25ml @16:33:31.65 Vacuum too high -- waiting up to 40s @16:33:38.61 Intake @16.2psia, vacuum from 9.7 to 13.7psi @16:33:38.63 SS.reconfigure SSinhale.with maxSpeed: 135 @16:33:38.70 SS.seek 25ml @16:33:41.96 Vacuum too high -- waiting up to 40s @16:33:48.70 Intake @16.4psia, vacuum from 9.9 to 13.9psi @16:33:48.72 SS.reconfigure SSinhale.with maxSpeed: 122 @16:33:48.80 SS.seek 25ml @16:33:52.04 Vacuum too high -- waiting up to 40s @16:33:58.53 Intake @16.5psia, vacuum from 10.0 to 14.0psi @16:33:58.55 SS.reconfigure SSinhale.with maxSpeed: 110 @16:33:58.62 SS.seek 25ml @16:34:01.78 Vacuum too high -- waiting up to 40s @16:34:07.61 Intake @16.2psia, vacuum from 9.7 to 13.7psi @16:34:07.63 SS.reconfigure SSinhale.with maxSpeed: 99 @16:34:07.70 SS.seek 25ml @16:34:10.50 Vacuum too high -- waiting up to 40s @16:34:15.38 SS.reconfigure SSinhale.with maxSpeed: 89 @16:34:15.44 SS.seek 25ml @16:34:15.76 ATV.dial :waste @16:34:15.77 ARV.dial between carrier and air1 @16:34:15.87 ASV.dial :waste1 @16:34:18.59
Vacuum too high -- waiting up to 40s @16:34:22.79 Intake @16.2psia, vacuum from 9.7 to 13.7psi @16:34:22.81 SS.reconfigure SSinhale.with maxSpeed: 80 @16:34:22.89 SS.seek 25ml @16:34:24.37 Stroke completed -- waiting for pressure to equalize ... @16:34:27.44 Intake.select :close @16:34:27.60 SSV.dial :exhaust @16:34:28.47 Exhaust.select :open @16:34:28.59 SS.reconfigure SSexhale @16:34:28.66 SS.seek 0ml @16:34:38.11 Exhaust.select :close @16:34:38.23 SSV.dial CBV @16:34:38.25
CBV.dial SSV @16:34:39.12 Allowing 130s for 25.0ml of remaining 1175.0ml @16:34:39.14 Intake.select :open @16:34:41.32 Intake @16.0psia, vacuum from 9.5 to 13.5psi @16:34:41.34 SS.reconfigure SSinhale.with maxSpeed: 80 @16:34:41.41 SS.seek 25ml @16:34:45.43 Vacuum too high -- waiting up to 40s @16:34:49.14 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:34:49.16 SS.reconfigure SSinhale.with maxSpeed: 72 @16:34:49.23 SS.seek 25ml @16:34:53.81 Vacuum too high -- waiting up to 40s @16:34:58.48 Intake @16.0psia, vacuum from 9.5 to 13.5psi @16:34:58.50 SS.reconfigure SSinhale.with maxSpeed: 65 @16:34:58.57 SS.seek 25ml @16:35:03.46 Vacuum too high -- waiting up to 40s @16:35:07.90 Intake @16.1psia, vacuum from 9.6 to 13.6psi @16:35:07.92 SS.reconfigure SSinhale.with maxSpeed: 59 @16:35:07.99 SS.seek 25ml @16:35:13.68 Vacuum too high -- waiting up to 40s @16:35:17.86 SS.reconfigure SSinhale.with maxSpeed: 53 @16:35:17.92 SS.seek 25ml @16:35:24.78 Vacuum too high -- waiting up to 40s @16:35:29.21 Intake @16.1psia, vacuum from 9.6 to 13.6psi @16:35:29.23 SS.reconfigure SSinhale.with maxSpeed: 48 @16:35:29.30 SS.seek 25ml @16:35:35.65 Vacuum too high -- waiting up to 40s @16:35:39.42 SS.reconfigure SSinhale.with maxSpeed: 43 @16:35:39.48 SS.seek 25ml @16:35:40.84 Stroke completed -- waiting for pressure to equalize ... @16:35:43.30 Intake.select :close @16:35:43.45 SSV.dial :exhaust @16:35:44.31 Exhaust.select :open @16:35:44.44 SS.reconfigure SSexhale @16:35:44.51 SS.seek 0ml @16:35:54.20 Exhaust.select :close @16:35:54.33 SSV.dial CBV @16:35:54.35
CBV.dial SSV @16:35:55.23 Allowing 130s for 25.0ml of remaining 1150.0ml @16:35:55.25 Intake.select :open @16:35:57.43 Intake @15.9psia, vacuum from 9.4 to 13.4psi @16:35:57.44 SS.reconfigure SSinhale.with maxSpeed: 43 @16:35:57.52 SS.seek 25ml @16:36:06.46 Vacuum too high -- waiting up to 40s @16:36:09.47 Intake @15.9psia, vacuum from 9.4 to 13.4psi @16:36:09.49 SS.reconfigure SSinhale.with maxSpeed: 39 @16:36:09.57 SS.seek 25ml @16:36:21.49 Vacuum too high -- waiting up to 40s @16:36:24.54 Intake @15.9psia, vacuum from 9.4 to 13.4psi @16:36:24.56 SS.reconfigure SSinhale.with maxSpeed: 35 @16:36:24.63 SS.seek 25ml @16:36:49.40 Vacuum too high -- waiting up to 40s @16:36:52.91 Intake @16.0psia, vacuum from 9.5 to 13.5psi @16:36:52.93 SS.reconfigure SSinhale.with maxSpeed: 32 @16:36:53.00 SS.seek 25ml @16:36:56.55 Stroke completed -- waiting for pressure to equalize ... @16:36:59.03 Intake.select :close @16:36:59.17 SSV.dial :exhaust @16:37:00.03 Exhaust.select :open @16:37:00.15 SS.reconfigure SSexhale @16:37:00.22 SS.seek 0ml @16:37:09.80 Exhaust.select :close @16:37:09.93 SSV.dial CBV @16:37:09.95
CBV.dial SSV @16:37:10.84 Allowing 130s for 25.0ml of remaining 1125.0ml @16:37:10.86 Intake.select :open @16:37:13.04 Intake @15.9psia, vacuum from 9.4 to 13.4psi @16:37:13.05 SS.reconfigure SSinhale.with maxSpeed: 32 @16:37:13.13 SS.seek 25ml @16:38:07.92 Stroke completed -- waiting for pressure to equalize ... @16:38:13.25 Intake.select :close @16:38:13.42 SSV.dial :exhaust @16:38:14.30 Exhaust.select :open @16:38:14.43 SS.reconfigure SSexhale @16:38:14.50 SS.seek 0ml @16:38:24.06 Exhaust.select :close @16:38:24.19 SSV.dial CBV @16:38:24.21
CBV.dial SSV @16:38:25.11 Allowing 130s for 25.0ml of remaining 1100.0ml @16:38:25.13 Intake.select :open @16:38:27.31 Intake @15.9psia, vacuum from 9.4 to 13.4psi @16:38:27.33 SS.reconfigure SSinhale.with maxSpeed: 32 @16:38:27.41 SS.seek 25ml @16:39:22.40 Stroke completed -- waiting for pressure to equalize ... @16:39:27.90 Intake.select :close @16:39:28.05 SSV.dial :exhaust @16:39:28.92 Exhaust.select :open @16:39:29.05 SS.reconfigure SSexhale @16:39:29.12 SS.seek 0ml @16:39:38.65 Exhaust.select :close @16:39:38.77 SSV.dial CBV @16:39:38.79
CBV.dial SSV @16:39:39.67 Allowing 130s for 25.0ml of remaining 1075.0ml @16:39:39.69 Intake.select :open @16:39:41.87 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:39:41.89 SS.reconfigure SSinhale.with maxSpeed: 32 @16:39:41.97 SS.seek 25ml @16:40:36.95 Stroke completed -- waiting for pressure to equalize ... @16:40:41.82 Intake.select :close @16:40:41.98 SSV.dial :exhaust @16:40:42.84 Exhaust.select :open @16:40:42.96 SS.reconfigure SSexhale @16:40:43.03 SS.seek 0ml @16:40:52.56 Exhaust.select :close @16:40:52.68 SSV.dial CBV @16:40:52.70
CBV.dial SSV @16:40:53.59 Allowing 130s for 25.0ml of remaining 1050.0ml @16:40:53.61 Intake.select :open @16:40:55.79 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:40:55.80 SS.reconfigure SSinhale.with maxSpeed: 32 @16:40:55.88 SS.seek 25ml @16:41:50.79 Stroke completed -- waiting for pressure to equalize ... @16:41:56.28 Intake.select :close @16:41:56.44 SSV.dial :exhaust @16:41:57.31 Exhaust.select :open @16:41:57.43 SS.reconfigure SSexhale @16:41:57.50 SS.seek 0ml @16:42:06.79 Exhaust.select :close @16:42:06.91 SSV.dial CBV @16:42:06.93
CBV.dial SSV @16:42:07.84 Allowing 130s for 25.0ml of remaining 1025.0ml @16:42:07.86 Intake.select :open @16:42:10.04 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:42:10.06 SS.reconfigure SSinhale.with maxSpeed: 32 @16:42:10.13 SS.seek 25ml @16:43:05.70 Stroke completed -- waiting for pressure to equalize ... @16:43:11.04 Intake.select :close @16:43:11.20 SSV.dial :exhaust @16:43:12.08 Exhaust.select :open @16:43:12.21 SS.reconfigure SSexhale @16:43:12.28 SS.seek 0ml @16:43:21.53 Exhaust.select :close @16:43:21.66 SSV.dial CBV @16:43:21.68
CBV.dial SSV @16:43:22.59 Allowing 130s for 25.0ml of remaining 1000.0ml @16:43:22.61 Intake.select :open @16:43:24.79 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:43:24.81 SS.reconfigure SSinhale.with maxSpeed: 32 @16:43:24.88 SS.seek 25ml @16:44:20.48 Stroke completed -- waiting for pressure to equalize ... @16:44:25.96 Intake.select :close @16:44:26.12 SSV.dial :exhaust @16:44:27.00 Exhaust.select :open @16:44:27.13 SS.reconfigure SSexhale @16:44:27.20 SS.seek 0ml @16:44:36.75 Exhaust.select :close @16:44:36.87 SSV.dial CBV @16:44:36.89
CBV.dial SSV @16:44:37.78 Allowing 130s for 25.0ml of remaining 975.0ml @16:44:37.80 Intake.select :open @16:44:39.98 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:44:39.99 SS.reconfigure SSinhale.with maxSpeed: 32 @16:44:40.07 SS.seek 25ml @16:45:35.17 Stroke completed -- waiting for pressure to equalize ... @16:45:40.65 Intake.select :close @16:45:40.82 SSV.dial :exhaust @16:45:41.67 Exhaust.select :open @16:45:41.79 SS.reconfigure SSexhale @16:45:41.86 SS.seek 0ml @16:45:51.56 Exhaust.select :close @16:45:51.68 SSV.dial CBV @16:45:51.70
CBV.dial SSV @16:45:52.57 Allowing 130s for 25.0ml of remaining 950.0ml @16:45:52.59 Intake.select :open @16:45:54.77 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:45:54.78 SS.reconfigure SSinhale.with maxSpeed: 32 @16:45:54.86 SS.seek 25ml @16:46:49.76 Stroke completed -- waiting for pressure to equalize ... @16:46:55.25 Intake.select :close @16:46:55.40 SSV.dial :exhaust @16:46:56.28 Exhaust.select :open @16:46:56.41 SS.reconfigure SSexhale @16:46:56.48 SS.seek 0ml @16:47:06.25 Exhaust.select :close @16:47:06.37 SSV.dial CBV @16:47:06.39
CBV.dial SSV @16:47:07.28 Allowing 130s for 25.0ml of remaining 925.0ml @16:47:07.30 Intake.select :open @16:47:09.48 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:47:09.50 SS.reconfigure SSinhale.with maxSpeed: 32 @16:47:09.57 SS.seek 25ml @16:48:04.36 Stroke completed -- waiting for pressure to equalize ... @16:48:09.71 Intake.select :close @16:48:09.87 SSV.dial :exhaust @16:48:10.75 Exhaust.select :open @16:48:10.88 SS.reconfigure SSexhale @16:48:10.95 SS.seek 0ml @16:48:20.59 Exhaust.select :close @16:48:20.71 SSV.dial CBV @16:48:20.73
CBV.dial SSV @16:48:21.62 Allowing 130s for 25.0ml of remaining 900.0ml @16:48:21.63 Intake.select :open @16:48:23.82 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:48:23.84 SS.reconfigure SSinhale.with maxSpeed: 32 @16:48:23.91 SS.seek 25ml @16:49:18.78 Stroke completed -- waiting for pressure to equalize ... @16:49:24.25 Intake.select :close @16:49:24.40 SSV.dial :exhaust @16:49:25.26 Exhaust.select :open @16:49:25.39 SS.reconfigure SSexhale @16:49:25.46 SS.seek 0ml @16:49:34.75 Exhaust.select :close @16:49:34.87 SSV.dial CBV @16:49:34.89
CBV.dial SSV @16:49:35.78 Allowing 130s for 25.0ml of remaining 875.0ml @16:49:35.80 Intake.select :open @16:49:37.98 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:49:37.99 SS.reconfigure SSinhale.with maxSpeed: 32 @16:49:38.07 SS.seek 25ml @16:50:33.40 Stroke completed -- waiting for pressure to equalize ... @16:50:39.52 Intake.select :close @16:50:39.66 SSV.dial :exhaust @16:50:40.54 Exhaust.select :open @16:50:40.67 SS.reconfigure SSexhale @16:50:40.74 SS.seek 0ml @16:50:50.31 Exhaust.select :close @16:50:50.43 SSV.dial CBV @16:50:50.45
CBV.dial SSV @16:50:51.34 Allowing 130s for 25.0ml of remaining 850.0ml @16:50:51.35 Intake.select :open @16:50:53.55 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:50:53.56 SS.reconfigure SSinhale.with maxSpeed: 32 @16:50:53.64 SS.seek 25ml @16:51:48.65 Stroke completed -- waiting for pressure to equalize ... @16:51:54.77 Intake.select :close @16:51:54.92 SSV.dial :exhaust @16:51:55.79 Exhaust.select :open @16:51:55.91 SS.reconfigure SSexhale @16:51:55.98 SS.seek 0ml @16:52:06.09 Exhaust.select :close @16:52:06.21 SSV.dial CBV @16:52:06.23
CBV.dial SSV @16:52:07.12 Allowing 130s for 25.0ml of remaining 825.0ml @16:52:07.14 Intake.select :open @16:52:09.32 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:52:09.34 SS.reconfigure SSinhale.with maxSpeed: 32 @16:52:09.41 SS.seek 25ml @16:53:04.35 Stroke completed -- waiting for pressure to equalize ... @16:53:10.46 Intake.select :close @16:53:10.61 SSV.dial :exhaust @16:53:11.46 Exhaust.select :open @16:53:11.59 SS.reconfigure SSexhale @16:53:11.66 SS.seek 0ml @16:53:21.24 Exhaust.select :close @16:53:21.37 SSV.dial CBV @16:53:21.39
CBV.dial SSV @16:53:22.28 Allowing 130s for 25.0ml of remaining 800.0ml @16:53:22.30 Intake.select :open @16:53:24.48 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:53:24.49 SS.reconfigure SSinhale.with maxSpeed: 32 @16:53:24.57 SS.seek 25ml @16:54:19.78 Stroke completed -- waiting for pressure to equalize ... @16:54:25.89 Intake.select :close @16:54:26.04 SSV.dial :exhaust @16:54:26.92 Exhaust.select :open @16:54:27.04 SS.reconfigure SSexhale @16:54:27.11 SS.seek 0ml @16:54:36.37 Exhaust.select :close @16:54:36.49 SSV.dial CBV @16:54:36.51
CBV.dial SSV @16:54:37.42 Allowing 130s for 25.0ml of remaining 775.0ml @16:54:37.44 Intake.select :open @16:54:39.62 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:54:39.63 SS.reconfigure SSinhale.with maxSpeed: 32 @16:54:39.71 SS.seek 25ml @16:55:34.84 Stroke completed -- waiting for pressure to equalize ... @16:55:41.57 Intake.select :close @16:55:41.72 SSV.dial :exhaust @16:55:42.60 Exhaust.select :open @16:55:42.73 SS.reconfigure SSexhale @16:55:42.80 SS.seek 0ml @16:55:52.06 Exhaust.select :close @16:55:52.18 SSV.dial CBV @16:55:52.20
CBV.dial SSV @16:55:53.10 Allowing 130s for 25.0ml of remaining 750.0ml @16:55:53.12 Intake.select :open @16:55:55.30 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:55:55.32 SS.reconfigure SSinhale.with maxSpeed: 32 @16:55:55.39 SS.seek 25ml @16:56:51.07 Stroke completed -- waiting for pressure to equalize ... @16:56:58.21 Intake.select :close @16:56:58.38 SSV.dial :exhaust @16:56:59.24 Exhaust.select :open @16:56:59.37 SS.reconfigure SSexhale @16:56:59.44 SS.seek 0ml @16:57:09.04 Exhaust.select :close @16:57:09.17 SSV.dial CBV @16:57:09.19
CBV.dial SSV @16:57:10.07 Allowing 130s for 25.0ml of remaining 725.0ml @16:57:10.09 Intake.select :open @16:57:12.27 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:57:12.29 SS.reconfigure SSinhale.with maxSpeed: 32 @16:57:12.36 SS.seek 25ml @16:58:07.46 Stroke completed -- waiting for pressure to equalize ... @16:58:14.13 Intake.select :close @16:58:14.28 SSV.dial :exhaust @16:58:15.13 Exhaust.select :open @16:58:15.26 SS.reconfigure SSexhale @16:58:15.33 SS.seek 0ml @16:58:25.06 Exhaust.select :close @16:58:25.18 SSV.dial CBV @16:58:25.20
CBV.dial SSV @16:58:26.09 Allowing 130s for 25.0ml of remaining 700.0ml @16:58:26.11 Intake.select :open @16:58:28.32 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:58:28.33 SS.reconfigure SSinhale.with maxSpeed: 32 @16:58:28.41 SS.seek 25ml @16:59:23.29 Stroke completed -- waiting for pressure to equalize ... @16:59:29.99 Intake.select :close @16:59:30.15 SSV.dial :exhaust @16:59:31.02 Exhaust.select :open @16:59:31.15 SS.reconfigure SSexhale @16:59:31.22 SS.seek 0ml @16:59:40.88 Exhaust.select :close @16:59:41.00 SSV.dial CBV @16:59:41.02
CBV.dial SSV @16:59:41.93 Allowing 130s for 25.0ml of remaining 675.0ml @16:59:41.95 Intake.select :open @16:59:44.13 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:59:44.15 SS.reconfigure SSinhale.with maxSpeed: 32 @16:59:44.22 SS.seek 25ml @17:00:39.52 Stroke completed -- waiting for pressure to equalize ... @17:00:46.28 Intake.select :close @17:00:46.44 SSV.dial :exhaust @17:00:47.31 Exhaust.select :open @17:00:47.44 SS.reconfigure SSexhale @17:00:47.51 SS.seek 0ml @17:00:57.18 Exhaust.select :close @17:00:57.31 SSV.dial CBV @17:00:57.33
CBV.dial SSV @17:00:58.23 Allowing 130s for 25.0ml of remaining 650.0ml @17:00:58.25 Intake.select :open @17:01:00.43 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:01:00.45 SS.reconfigure SSinhale.with maxSpeed: 32 @17:01:00.52 SS.seek 25ml @17:01:55.48 Stroke completed -- waiting for pressure to equalize ... @17:02:02.17 Intake.select :close @17:02:02.33 SSV.dial :exhaust @17:02:03.20 Exhaust.select :open @17:02:03.33 SS.reconfigure SSexhale @17:02:03.40 SS.seek 0ml @17:02:13.29 Exhaust.select :close @17:02:13.42 SSV.dial CBV @17:02:13.44
CBV.dial SSV @17:02:14.32 Allowing 130s for 25.0ml of remaining 625.0ml @17:02:14.34 Intake.select :open @17:02:16.52 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:02:16.53 SS.reconfigure SSinhale.with maxSpeed: 32 @17:02:16.61 SS.seek 25ml @17:03:11.79 Stroke completed -- waiting for pressure to equalize ... @17:03:18.49 Intake.select :close @17:03:18.64 SSV.dial :exhaust @17:03:19.51 Exhaust.select :open @17:03:19.64 SS.reconfigure SSexhale @17:03:19.71 SS.seek 0ml @17:03:29.40 Exhaust.select :close @17:03:29.53 SSV.dial CBV @17:03:29.55
CBV.dial SSV @17:03:30.43 Allowing 130s for 25.0ml of remaining 600.0ml @17:03:30.44 Intake.select :open @17:03:32.63 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:03:32.64 SS.reconfigure SSinhale.with maxSpeed: 32 @17:03:33.13 SS.seek 25ml @17:04:28.27 Stroke completed -- waiting for pressure to equalize ... @17:04:35.61 Intake.select :close @17:04:35.76 SSV.dial :exhaust @17:04:36.63 Exhaust.select :open @17:04:36.76 SS.reconfigure SSexhale @17:04:36.83 SS.seek 0ml @17:04:46.54 Exhaust.select :close @17:04:46.67 SSV.dial CBV @17:04:46.69
CBV.dial SSV @17:04:47.59 Allowing 130s for 25.0ml of remaining 575.0ml @17:04:47.61 Intake.select :open @17:04:49.79 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:04:49.81 SS.reconfigure SSinhale.with maxSpeed: 32 @17:04:49.88 SS.seek 25ml @17:05:42.24 Vacuum too high -- waiting up to 40s @17:05:46.92 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:05:46.94 SS.reconfigure SSinhale.with maxSpeed: 29 @17:05:47.01 SS.seek 25ml @17:05:50.41 Stroke completed -- waiting for pressure to equalize ... @17:05:52.88 Intake.select :close @17:05:53.04 SSV.dial :exhaust @17:05:53.91 Exhaust.select :open @17:05:54.04 SS.reconfigure SSexhale @17:05:54.11 SS.seek 0ml @17:06:03.69 Exhaust.select :close @17:06:03.81 SSV.dial CBV @17:06:03.83
CBV.dial SSV @17:06:04.71 Allowing 130s for 25.0ml of remaining 550.0ml @17:06:04.73 Intake.select :open @17:06:06.90 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:06:06.91 SS.reconfigure SSinhale.with maxSpeed: 29 @17:06:06.99 SS.seek 25ml @17:07:07.74 Stroke completed -- waiting for pressure to equalize ... @17:07:13.09 Intake.select :close @17:07:13.24 SSV.dial :exhaust @17:07:14.10 Exhaust.select :open @17:07:14.23 SS.reconfigure SSexhale @17:07:14.30 SS.seek 0ml @17:07:23.82 Exhaust.select :close @17:07:23.95 SSV.dial CBV @17:07:23.97
CBV.dial SSV @17:07:24.85 Allowing 130s for 25.0ml of remaining 525.0ml @17:07:24.87 Intake.select :open @17:07:27.05 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:07:27.07 SS.reconfigure SSinhale.with maxSpeed: 29 @17:07:27.14 SS.seek 25ml @17:08:27.76 Stroke completed -- waiting for pressure to equalize ... @17:08:33.24 Intake.select :close @17:08:33.40 SSV.dial :exhaust @17:08:34.27 Exhaust.select :open @17:08:34.40 SS.reconfigure SSexhale @17:08:34.47 SS.seek 0ml @17:08:44.24 Exhaust.select :close @17:08:44.37 SSV.dial CBV @17:08:44.39
CBV.dial SSV @17:08:45.29 Allowing 130s for 25.0ml of remaining 500.0ml @17:08:45.31 Intake.select :open @17:08:47.49 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:08:47.50 SS.reconfigure SSinhale.with maxSpeed: 29 @17:08:47.58 SS.seek 25ml @17:09:48.08 Stroke completed -- waiting for pressure to equalize ... @17:09:52.99 Intake.select :close @17:09:53.15 SSV.dial :exhaust @17:09:54.02 Exhaust.select :open @17:09:54.15 SS.reconfigure SSexhale @17:09:54.22 SS.seek 0ml @17:10:03.79 Exhaust.select :close @17:10:03.91 SSV.dial CBV @17:10:03.93
CBV.dial SSV @17:10:04.83 Allowing 130s for 25.0ml of remaining 475.0ml @17:10:04.85 Intake.select :open @17:10:07.03 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:10:07.05 SS.reconfigure SSinhale.with maxSpeed: 29 @17:10:07.12 SS.seek 25ml @17:11:08.13 Stroke completed -- waiting for pressure to equalize ... @17:11:14.02 Intake.select :close @17:11:14.17 SSV.dial :exhaust @17:11:15.04 Exhaust.select :open @17:11:15.17 SS.reconfigure SSexhale @17:11:15.24 SS.seek 0ml @17:11:24.57 Exhaust.select :close @17:11:24.70 SSV.dial CBV @17:11:24.72
CBV.dial SSV @17:11:25.62 Allowing 130s for 25.0ml of remaining 450.0ml @17:11:25.63 Intake.select :open @17:11:27.83 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:11:27.85 SS.reconfigure SSinhale.with maxSpeed: 29 @17:11:27.92 SS.seek 25ml @17:12:29.19 Stroke completed -- waiting for pressure to equalize ... @17:12:35.29 Intake.select :close @17:12:35.45 SSV.dial :exhaust @17:12:36.30 Exhaust.select :open @17:12:36.43 SS.reconfigure SSexhale @17:12:36.50 SS.seek 0ml @17:12:45.79 Exhaust.select :close @17:12:45.92 SSV.dial CBV @17:12:45.94
CBV.dial SSV @17:12:46.83 Allowing 130s for 25.0ml of remaining 425.0ml @17:12:46.85 Intake.select :open @17:12:49.03 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:12:49.05 SS.reconfigure SSinhale.with maxSpeed: 29 @17:12:49.12 SS.seek 25ml @17:13:50.35 Stroke completed -- waiting for pressure to equalize ... @17:13:57.03 Intake.select :close @17:13:57.19 SSV.dial :exhaust @17:13:58.05 Exhaust.select :open @17:13:58.18 SS.reconfigure SSexhale @17:13:58.25 SS.seek 0ml @17:14:07.77 Exhaust.select :close @17:14:07.89 SSV.dial CBV @17:14:07.91
CBV.dial SSV @17:14:08.80 Allowing 130s for 25.0ml of remaining 400.0ml @17:14:08.82 Intake.select :open @17:14:11.00 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:14:11.01 SS.reconfigure SSinhale.with maxSpeed: 29 @17:14:11.09 SS.seek 25ml @17:15:11.75 Stroke completed -- waiting for pressure to equalize ... @17:15:17.86 Intake.select :close @17:15:18.34 SSV.dial :exhaust @17:15:19.29 Exhaust.select :open @17:15:19.42 SS.reconfigure SSexhale @17:15:19.49 SS.seek 0ml @17:15:29.19 Exhaust.select :close @17:15:29.32 SSV.dial CBV @17:15:29.34
CBV.dial SSV @17:15:30.24 Allowing 130s for 25.0ml of remaining 375.0ml @17:15:30.25 Intake.select :open @17:15:32.45 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:15:32.47 SS.reconfigure SSinhale.with maxSpeed: 29 @17:15:32.55 SS.seek 25ml @17:16:33.07 Stroke completed -- waiting for pressure to equalize ... @17:16:39.17 Intake.select :close @17:16:39.33 SSV.dial :exhaust @17:16:40.21 Exhaust.select :open @17:16:40.34 SS.reconfigure SSexhale @17:16:40.41 SS.seek 0ml @17:16:50.25 Exhaust.select :close @17:16:50.38 SSV.dial CBV @17:16:50.40
CBV.dial SSV @17:16:51.29 Allowing 130s for 25.0ml of remaining 350.0ml @17:16:51.31 Intake.select :open @17:16:53.49 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:16:53.50 SS.reconfigure SSinhale.with maxSpeed: 29 @17:16:53.58 SS.seek 25ml @17:17:54.08 Stroke completed -- waiting for pressure to equalize ... @17:18:00.15 Intake.select :close @17:18:00.30 SSV.dial :exhaust @17:18:01.16 Exhaust.select :open @17:18:01.29 SS.reconfigure SSexhale @17:18:01.36 SS.seek 0ml @17:18:10.60 Exhaust.select :close @17:18:10.72 SSV.dial CBV @17:18:10.74
CBV.dial SSV @17:18:11.63 Allowing 130s for 25.0ml of remaining 325.0ml @17:18:11.65 Intake.select :open @17:18:13.82 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:18:13.83 SS.reconfigure SSinhale.with maxSpeed: 29 @17:18:13.91 SS.seek 25ml @17:19:15.16 Stroke completed -- waiting for pressure to equalize ... @17:19:22.49 Intake.select :close @17:19:22.65 SSV.dial :exhaust @17:19:23.91 Exhaust.select :open @17:19:24.04 SS.reconfigure SSexhale @17:19:24.11 SS.seek 0ml @17:19:33.64 Exhaust.select :close @17:19:33.77 SSV.dial CBV @17:19:33.79
CBV.dial SSV @17:19:34.68 Allowing 130s for 25.0ml of remaining 300.0ml @17:19:34.70 Intake.select :open @17:19:36.88 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:19:36.90 SS.reconfigure SSinhale.with maxSpeed: 29 @17:19:36.98 SS.seek 25ml @17:20:37.72 Stroke completed -- waiting for pressure to equalize ... @17:20:44.42 Intake.select :close @17:20:44.58 SSV.dial :exhaust @17:20:45.46 Exhaust.select :open @17:20:45.59 SS.reconfigure SSexhale @17:20:45.66 SS.seek 0ml @17:20:55.32 Exhaust.select :close @17:20:55.45 SSV.dial CBV @17:20:55.47
CBV.dial SSV @17:20:56.36 Allowing 130s for 25.0ml of remaining 275.0ml @17:20:56.38 Intake.select :open @17:20:58.56 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:20:58.58 SS.reconfigure SSinhale.with maxSpeed: 29 @17:20:58.65 SS.seek 25ml @17:21:59.47 Stroke completed -- waiting for pressure to equalize ... @17:22:06.75 Intake.select :close @17:22:06.90 SSV.dial :exhaust @17:22:07.75 Exhaust.select :open @17:22:07.88 SS.reconfigure SSexhale @17:22:07.95 SS.seek 0ml @17:22:17.80 Exhaust.select :close @17:22:17.93 SSV.dial CBV @17:22:17.95
CBV.dial SSV @17:22:18.83 Allowing 130s for 25.0ml of remaining 250.0ml @17:22:18.85 Intake.select :open @17:22:21.03 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:22:21.04 SS.reconfigure SSinhale.with maxSpeed: 29 @17:22:21.12 SS.seek 25ml @17:23:21.71 Stroke completed -- waiting for pressure to equalize ... @17:23:27.85 Intake.select :close @17:23:28.41 SSV.dial :exhaust @17:23:29.27 Exhaust.select :open @17:23:29.40 SS.reconfigure SSexhale @17:23:29.47 SS.seek 0ml @17:23:39.27 Exhaust.select :close @17:23:39.40 SSV.dial CBV @17:23:39.42
CBV.dial SSV @17:23:40.30 Allowing 130s for 25.0ml of remaining 225.0ml @17:23:40.31 Intake.select :open @17:23:42.51 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:23:42.53 SS.reconfigure SSinhale.with maxSpeed: 29 @17:23:42.60 SS.seek 25ml @17:24:43.14 Stroke completed -- waiting for pressure to equalize ... @17:24:49.82 Intake.select :close @17:24:49.98 SSV.dial :exhaust @17:24:50.85 Exhaust.select :open @17:24:50.98 SS.reconfigure SSexhale @17:24:51.05 SS.seek 0ml @17:25:00.69 Exhaust.select :close @17:25:00.82 SSV.dial CBV @17:25:00.84
CBV.dial SSV @17:25:01.74 Allowing 130s for 25.0ml of remaining 200.0ml @17:25:01.76 Intake.select :open @17:25:03.94 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:25:03.96 SS.reconfigure SSinhale.with maxSpeed: 29 @17:25:04.03 SS.seek 25ml @17:26:04.63 Stroke completed -- waiting for pressure to equalize ... @17:26:11.95 Intake.select :close @17:26:12.10 SSV.dial :exhaust @17:26:12.96 Exhaust.select :open @17:26:13.09 SS.reconfigure SSexhale @17:26:13.16 SS.seek 0ml @17:26:22.75 Exhaust.select :close @17:26:22.87 SSV.dial CBV @17:26:22.89
CBV.dial SSV @17:26:23.78 Allowing 130s for 25.0ml of remaining 175.0ml @17:26:23.80 Intake.select :open @17:26:25.98 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:26:25.99 SS.reconfigure SSinhale.with maxSpeed: 29 @17:26:26.07 SS.seek 25ml @17:27:26.74 Stroke completed -- waiting for pressure to equalize ... @17:27:34.47 Intake.select :close @17:27:34.64 SSV.dial :exhaust @17:27:35.50 Exhaust.select :open @17:27:35.63 SS.reconfigure SSexhale @17:27:35.70 SS.seek 0ml @17:27:45.33 Exhaust.select :close @17:27:45.46 SSV.dial CBV @17:27:45.48
CBV.dial SSV @17:27:46.36 Allowing 130s for 25.0ml of remaining 150.0ml @17:27:46.38 Intake.select :open @17:27:48.56 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:27:48.58 SS.reconfigure SSinhale.with maxSpeed: 29 @17:27:48.66 SS.seek 25ml @17:28:45.61 Vacuum too high -- waiting up to 40s @17:28:50.52 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:28:50.54 SS.reconfigure SSinhale.with maxSpeed: 26 @17:28:50.62 SS.seek 25ml @17:28:55.33 Stroke completed -- waiting for pressure to equalize ... @17:28:59.02 Intake.select :close @17:28:59.19 SSV.dial :exhaust @17:29:00.06 Exhaust.select :open @17:29:00.19 SS.reconfigure SSexhale @17:29:00.26 SS.seek 0ml @17:29:09.88 Exhaust.select :close @17:29:10.00 SSV.dial CBV @17:29:10.02
CBV.dial SSV @17:29:10.92 Allowing 130s for 25.0ml of remaining 125.0ml @17:29:10.94 Intake.select :open @17:29:13.12 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:29:13.13 SS.reconfigure SSinhale.with maxSpeed: 26 @17:29:13.21 SS.seek 25ml @17:30:21.05 Stroke completed -- waiting for pressure to equalize ... @17:30:26.55 Intake.select :close @17:30:27.09 SSV.dial :exhaust @17:30:27.99 Exhaust.select :open @17:30:28.12 SS.reconfigure SSexhale @17:30:28.19 SS.seek 0ml @17:30:37.77 Exhaust.select :close @17:30:37.90 SSV.dial CBV @17:30:37.92
CBV.dial SSV @17:30:38.81 Allowing 130s for 25.0ml of remaining 100.0ml @17:30:38.83 Intake.select :open @17:30:41.01 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:30:41.03 SS.reconfigure SSinhale.with maxSpeed: 26 @17:30:41.11 SS.seek 25ml @17:31:48.67 Stroke completed -- waiting for pressure to equalize ... @17:31:54.76 Intake.select :close @17:31:54.90 SSV.dial :exhaust @17:31:55.77 Exhaust.select :open @17:31:55.90 SS.reconfigure SSexhale @17:31:55.97 SS.seek 0ml @17:32:05.61 Exhaust.select :close @17:32:05.73 SSV.dial CBV @17:32:05.75
CBV.dial SSV @17:32:06.64 Allowing 130s for 25.0ml of remaining 75.0ml @17:32:06.66 Intake.select :open @17:32:08.84 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:32:08.86 SS.reconfigure SSinhale.with maxSpeed: 26 @17:32:08.93 SS.seek 25ml @17:33:16.53 Stroke completed -- waiting for pressure to equalize ... @17:33:22.01 Intake.select :close @17:33:22.16 SSV.dial :exhaust @17:33:23.05 Exhaust.select :open @17:33:23.18 SS.reconfigure SSexhale @17:33:23.25 SS.seek 0ml @17:33:32.50 Exhaust.select :close @17:33:32.62 SSV.dial CBV @17:33:32.64
CBV.dial SSV @17:33:33.53 Allowing 130s for 25.0ml of remaining 50.0ml @17:33:33.55 Intake.select :open @17:33:35.73 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:33:35.75 SS.reconfigure SSinhale.with maxSpeed: 26 @17:33:35.82 SS.seek 25ml @17:34:43.53 Stroke completed -- waiting for pressure to equalize ... @17:34:50.25 Intake.select :close @17:34:50.39 SSV.dial :exhaust @17:34:51.27 Exhaust.select :open @17:34:51.40 SS.reconfigure SSexhale @17:34:51.87 SS.seek 0ml @17:35:01.61 Exhaust.select :close @17:35:01.74 SSV.dial CBV @17:35:01.76
CBV.dial SSV @17:35:02.66 Allowing 130s for 25.0ml of remaining 25.0ml @17:35:02.68 Intake.select :open @17:35:04.86 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:35:04.88 SS.reconfigure SSinhale.with maxSpeed: 26 @17:35:04.96 SS.seek 24.96ml @17:36:12.45 Stroke completed -- waiting for pressure to equalize ... @17:36:18.56 Intake.select :close @17:36:18.71 SSV.dial :exhaust @17:36:19.58 Exhaust.select :open @17:36:19.71 SS.reconfigure SSexhale @17:36:19.78 SS.seek 0ml @17:36:29.28 Exhaust.select :close @17:36:29.41 Sampled 1200.0ml @17:36:29.42 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @17:36:29.44 Intake.select :close @17:36:29.53 CSV.dial :waste @17:36:29.61 CS.seek 0ml @17:36:29.70 SSV.dial CBV,avoiding: :exhaust @17:36:29.73
CBV.dial SSV @17:36:29.80 CSV.dial :air @17:36:30.80 CS.seek 10ml @17:36:31.91
CTV.dial :air @17:36:32.79 delay 15 seconds @17:36:47.81 SSV.dial :spit,avoiding: :exhaust @17:36:49.34 CTV.dial CSV,avoiding: :intake @17:36:49.43
SS.seek 0ml @17:36:49.52 SSV.dial CBV,avoiding: :exhaust @17:36:58.27 CBV.dial between SSV and waste2 @17:36:58.69 CTV.dial CSV,avoiding: :air @17:36:58.72
CSV.dial CTV @17:36:59.28 CS.reconfigure CSpressurize @17:36:59.34 CS.seek 0ml @17:37:25.62 CSV.dial :air @17:37:26.16 CS.reconfigure CSfast @17:37:26.23 CS.seek 10ml @17:37:52.95 CSV.dial between air and CTV @17:37:53.33 CS.seek 2.548ml @17:38:13.65 CTV.dial CSV,avoiding: :air @17:38:13.69
CSV.dial CTV @17:38:14.03 CS.reconfigure CSpressurize @17:38:14.09 CS.seek 0ml @17:38:18.11 BubblePoint: Intake @70.0psia, Outlet @14.5psia @17:38:18.12 CBV.dial SSV @17:38:18.52 SS.reconfigure SSconfig @17:38:18.58 SS.seek 10ml @17:38:24.56 delay 15 seconds @17:38:39.68 Evacuated: Intake @34.4psia, Outlet @14.5psia @17:38:39.69 SSV.dial :spit,avoiding: :exhaust @17:38:41.23 SS.reconfigure SSexhale @17:38:41.29 SS.seek 0ml @17:38:46.83 SS.reconfigure SSfast @17:38:46.90 SS.seek 5ml @17:38:49.70 SSV.dial CBV,avoiding: :exhaust @17:38:49.73
CBV.dial SSV @17:38:51.23 CTV.dial :air,avoiding: :intake @17:38:52.80 CSV.dial CTV @17:38:52.83
SS.seek 10ml @17:38:53.83 CSV.dial :air @17:38:54.35 CS.reconfigure CSfast @17:38:54.41 CS.seek 0ml @17:38:55.56
SSV.dial :spit,avoiding: :exhaust @17:38:57.09 SS.reconfigure SSexhale @17:38:57.15 SS.seek 0ml @17:38:58.59 CS.coast @17:39:02.47
SS.reconfigure SSfast @17:39:02.54 SS.seek 5ml @17:39:05.42 SSV.dial CBV,avoiding: :exhaust @17:39:05.45
CBV.dial SSV @17:39:07.99 SSV.dial :spit,avoiding: :exhaust @17:39:09.52 SS.reconfigure SSconfig @17:39:09.58 SS.seek 0ml @17:39:12.64 SS.coast @17:39:12.70 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @17:39:12.71 Intake.select :close @17:39:12.80 CSV.dial :waste @17:39:12.93 SSV.dial CBV,avoiding: :exhaust @17:39:12.97
CBV.dial SSV @17:39:13.71 CS.seek 0ml @17:39:13.81 CSV.dial :air @17:39:14.50
CTV.dial :air @17:39:14.52 delay 15 seconds @17:39:14.67 CS.seek 10ml @17:39:29.54
SSV.dial :spit,avoiding: :exhaust @17:39:31.06 CTV.dial CSV,avoiding: :intake @17:39:31.12
SS.reconfigure SSexhale @17:39:31.21 SS.seek 0ml @17:39:31.28 SSV.dial CBV,avoiding: :exhaust @17:39:41.37 CBV.dial between SSV and waste2 @17:39:41.80 CSV.dial between air and CTV @17:39:42.17 CS.seek 8.326ml @17:39:47.46 CTV.dial CSV,avoiding: :air @17:39:47.49
CSV.dial CTV @17:39:47.86 CS.reconfigure CSpressurize @17:39:47.92 CS.seek 0ml @17:40:09.96 CSV.dial :air @17:40:10.52 CS.reconfigure CSfast @17:40:10.58 CS.seek 10ml @17:40:37.07 CSV.dial between air and CTV @17:40:37.46 CS.seek 3.081ml @17:40:56.12 CTV.dial CSV,avoiding: :air @17:40:56.16
CSV.dial CTV @17:40:56.52 CS.reconfigure CSpressurize @17:40:56.58 CS.seek 0ml @17:41:04.37 BubblePoint: Intake @69.1psia, Outlet @14.0psia @17:41:04.38 CBV.dial SSV @17:41:04.79 SS.reconfigure SSconfig @17:41:04.85 SS.seek 10ml @17:41:10.25 delay 15 seconds @17:41:25.37 Evacuated: Intake @36.3psia, Outlet @17.2psia @17:41:25.76 SSV.dial :spit,avoiding: :exhaust @17:41:27.34 SS.reconfigure SSexhale @17:41:27.40 SS.seek 0ml @17:41:32.47 SS.reconfigure SSfast @17:41:32.54 SS.seek 5ml @17:41:35.33 SSV.dial CBV,avoiding: :exhaust @17:41:35.38
CBV.dial SSV @17:41:36.89 CTV.dial :air,avoiding: :intake @17:41:38.47 CSV.dial CTV @17:41:38.50
SS.seek 10ml @17:41:39.51 CSV.dial :air @17:41:40.07 CS.reconfigure CSfast @17:41:40.12 CS.seek 0ml @17:41:41.14
SSV.dial :spit,avoiding: :exhaust @17:41:42.07 CS.coast @17:41:42.68
SS.reconfigure SSexhale @17:41:42.74 SS.seek 0ml @17:41:48.22 SS.reconfigure SSfast @17:41:48.29 SS.seek 5ml @17:41:50.94 SSV.dial CBV,avoiding: :exhaust @17:41:50.97
CBV.dial SSV @17:41:53.51 SSV.dial :spit,avoiding: :exhaust @17:41:55.04 SS.reconfigure SSconfig @17:41:55.10 SS.seek 0ml @17:41:58.31 SS.coast @17:41:58.36 Lysing: Adding 1.5ml lysis @17:41:58.37 CBV.dial between CSV and waste1,avoiding: :DBV @17:42:00.12 CTV.dial CSV @17:42:01.65 CSV.dial CSR @17:42:02.56 Pulling 1ml of CSR.lysis @17:42:02.57 CSR.select :lysis @17:42:02.71 CS.reconfigure CSconfig @17:42:02.78 CS.seek 1ml @17:42:06.34 delay 5 seconds @17:42:11.36 CSR.select :air @17:42:11.53 CS.seek 6ml @17:42:25.24 delay 2 seconds @17:42:27.26 CSV.dial :waste1 @17:42:28.78 CS.reconfigure CSfast @17:42:28.85 CS.seek :empty @17:42:45.28 CSV.dial CSR @17:42:46.84 Pulling 1.5ml of CSR.lysis @17:42:46.85 CSR.select :lysis @17:42:46.99 CS.reconfigure CSconfig @17:42:47.06 CS.seek 1.5ml @17:42:51.93 delay 5 seconds @17:42:56.95 CSR.select :air @17:42:57.14 CS.seek 4.5ml @17:43:05.75 delay 2 seconds @17:43:07.77 CSV.dial CTV @17:43:08.30 CS.seek 1.275ml @17:43:17.60 Heating sh1 puck from tube 4 in Collection Clamp to 85C for 5:00 @17:43:17.62 CH.seek 85C,8:00 @17:46:49.11 CH.hold 5:00,85C @17:51:49.27 Collection Puck Heater at 85C after lysing @17:51:49.29 CS.seek 0ml @17:51:53.59 CTV.dial between spare and air,avoiding: :intake @17:51:54.81 CSV.dial :air @17:51:55.43 CS.seek 5ml @17:52:09.25 CSV.dial CTV @17:52:09.77 delay 2 seconds @17:52:11.79 CSV.dial :air @17:52:12.31 delay 2 seconds @17:52:14.33 CSV.dial CTV @17:52:14.86 delay 2 seconds @17:52:16.88 CSV.dial :air @17:52:17.41 delay 2 seconds @17:52:19.44 CS.seek 0ml @17:52:33.19 Recovering Lysate into Collection syringe @17:52:33.20 CSV.dial CBV @17:52:34.41 CBV.dial CSV @17:52:34.83 CS.seek 5ml @17:52:48.56 delay 5 seconds @17:52:53.58 CTV.dial :air @17:52:54.17 CS.seek 7ml @17:53:00.28 delay 5 seconds @17:53:05.30 CSV.dial :air2 @17:53:05.81 CS.seek 10ml @17:53:14.47 CSV.dial between air and CTV,avoiding: CBV @17:53:15.92 Lysing Complete @17:53:16.01 Storing sh1 puck in Collection Clamp to tube 3 @17:53:16.18 SC.reconfigure SCtight @17:53:16.24 SC.seek 3 @17:53:18.36 SC.reconfigure SCloose @17:53:19.02 Elbow.reconfigure TightElbow @17:53:19.08 Elbow.seek 3 @17:53:21.01 Forearm.reconfigure TightForearm @17:53:21.07 Forearm.seek 3 + 700 counts @17:53:22.20 SE.reconfigure SEpick @17:53:22.29 SE.seek beamBreak @17:53:25.30 SE.configure SEconfig @17:53:25.37 SE.seek :place @17:53:25.94 Forearm.reconfigure LooseForearm @17:53:26.00 Forearm.seek :retract @17:53:26.97 Elbow.seek CC @17:53:28.70 Elbow.reconfigure LooseElbow @17:53:28.81 Forearm.seek CC @17:53:29.73 Hand.close @17:53:30.45 CC.seek :open @17:53:34.91 Forearm.seek :retract @17:53:35.89 Elbow.reconfigure TightElbow @17:53:35.96 Elbow.seek 3 @17:53:37.91 Elbow.reconfigure LimpElbow @17:53:37.98 Forearm.seek 3 @17:53:39.03 Elbow.hold @17:53:39.11 Elbow.reconfigure TightElbow @17:53:39.17 Hand.open @17:53:40.06 Forearm.force -20 @17:53:40.12 Forearm.seek 3 + 350 counts @17:53:40.43 Forearm.reconfigure TightForearm @17:53:40.49 Forearm.seek 3 + 700 counts @17:53:40.85 SE.seek :down @17:53:41.97
Forearm.reconfigure LooseForearm @17:53:42.04 Forearm.seek :retract @17:53:43.74 SE.coast @17:53:43.79
SC.coast @17:53:43.84 Tranferring lysate to PRV @17:53:43.85 CSV.dial :air @17:53:44.23 CS.seek 10ml @17:53:44.31 CSV.dial between air and CTV @17:53:44.67 AIV.dial :bypass @17:53:44.88 Beginning samplePassC @17:53:44.90 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:53:45.01 Hand.open @17:53:45.43 Elbow.seek FlushPuck::Garage @17:53:47.39 Elbow.reconfigure LooseElbow @17:53:47.51 Forearm.seek :garage @17:53:48.55 Hand.close @17:53:49.80 Forearm.seek :retract @17:53:50.91 Elbow.reconfigure TightElbow @17:53:50.97 Elbow.seek CC @17:53:53.92 Elbow.reconfigure LooseElbow @17:53:54.03 Forearm.reconfigure TightForearm @17:53:54.09 Forearm.seek CC @17:53:55.30 CC.seek :closed @17:53:59.56 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.24 @17:53:59.65 Hand.open @17:54:00.50 Forearm.reconfigure LooseForearm @17:54:00.56 Forearm.seek :retract @17:54:01.56 AWV.select :air @17:54:01.57 AIV.dial :bypass @17:54:01.60 PSV.dial PSR @17:54:03.17 Pulling 1.5ml of PSR.flush @17:54:03.18 PSR.select :flush @17:54:03.33 PS.seek 1.5ml @17:54:19.81 delay 5 seconds @17:54:24.83 PSV.dial :waste1 @17:54:25.37 PS.seek 0ml @17:54:41.78 PSV.dial PSR @17:54:42.31 PSR.select :air @17:54:42.44 PS.seek 2.5ml @17:55:09.03 PRV.dial CBV @17:55:09.06
CBV.dial PRV @17:55:09.97 PSV.dial :mixloop @17:55:11.13 CTV.dial :air,avoiding: :intake @17:55:11.14 PTV.dial :PRVmixing @17:55:11.26 PS.seek 0ml @17:55:38.10 PSV.dial :air @17:55:38.62 PS.seek 2.5ml @17:56:05.53 PSV.dial :mixloop @17:56:06.06 PS.seek 0ml @17:56:32.72 PSV.dial :air @17:56:33.25 PS.seek 2.5ml @17:56:59.72 PSV.dial :mixloop @17:57:00.25 PS.seek 0ml @17:57:26.69 Garaging FlushPuck in Collection Clamp @17:57:26.78 Elbow.reconfigure TightElbow @17:57:26.84 Elbow.seek CC @17:57:27.05 Elbow.reconfigure LooseElbow @17:57:27.15 Forearm.seek CC @17:57:28.14 Hand.close @17:57:28.95 CC.seek :open @17:57:33.33 Forearm.seek :retract @17:57:34.35 Elbow.reconfigure TightElbow @17:57:34.42 Elbow.seek :garage @17:57:37.49 Elbow.reconfigure LooseElbow @17:57:37.60 Forearm.reconfigure TightForearm @17:57:37.65 Forearm.seek :garage @17:57:38.83 Hand.open @17:57:39.63 Forearm.reconfigure LooseForearm @17:57:39.69 Forearm.seek :retract @17:57:41.14 Connecting PS to CS to transfer sample @17:57:41.15 CBV.dial AIV @17:57:41.16 AIV.dial :bypass @17:57:41.19 PSV.dial :mixloop @17:57:41.22 PTV.dial :PRVmixing @17:57:41.24 PRV.dial AIV @17:57:41.27 CTV.dial :air,avoiding: :intake @17:57:41.30 Loading lyfil puck from tube 4 into Collection Clamp @17:57:41.54 SC.reconfigure SCtight @17:57:41.60 SC.seek 4 @17:57:43.69 SC.reconfigure SCloose @17:57:43.91 Elbow.reconfigure TightElbow @17:57:43.97 Elbow.seek 4 @17:57:46.01 Forearm.reconfigure TightForearm @17:57:46.08 Forearm.seek 4 + 700 counts @17:57:47.19 SE.reconfigure SEpick @17:57:47.28 SE.seek beamBreak @17:57:49.83 SE.configure SEconfig @17:57:49.99 Elbow.seek 4 @17:57:50.30 Forearm.seek 4 + 300 counts @17:57:50.52 Forearm.reconfigure LooseForearm @17:57:50.58 Hand.close @17:57:51.34 SE.seek :down @17:57:51.55
Forearm.seek :retract @17:57:52.49 Elbow.seek CC @17:57:53.49 SE.coast @17:57:54.24
Elbow.reconfigure LooseElbow @17:57:54.35 Forearm.reconfigure TightForearm @17:57:54.40 Forearm.seek CC @17:57:55.65 CC.seek :closed @17:57:59.86 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.35 @17:57:59.87 Hand.open @17:58:00.75 Forearm.reconfigure LooseForearm @17:58:00.81 Forearm.seek :retract @17:58:01.79 SC.coast @17:58:01.84 CTV.dial CSV,avoiding: :intake @17:58:03.38 CSV.dial CTV @17:58:03.73 CS.seek 5ml @17:58:17.54 delay 1:20 @17:59:37.57 CS.seek 2.5ml @17:59:45.04 delay 40 seconds @18:00:25.07 CS.seek 0ml @18:00:32.48 delay 1:00 @18:01:32.51 PSV.dial :air @18:01:33.05 PS.seek 2.5ml @18:01:59.66 PSV.dial :mixloop @18:02:00.18 delay 3 seconds @18:02:03.20 PSV.dial :air @18:02:03.71 delay 2 seconds @18:02:05.73 PSV.dial :mixloop @18:02:06.24 delay 3 seconds @18:02:09.26 PSV.dial :waste1 @18:02:10.76 PS.seek :empty @18:02:37.32 CBV.dial :waste1 @18:02:37.85 delay 5 seconds @18:02:42.87 CBV.dial AIV @18:02:43.40 delay 10 seconds @18:02:53.43 CBV.dial :waste1 @18:02:53.95 delay 10 seconds @18:03:04.40 CBV.dial AIV @18:03:04.98 delay 10 seconds @18:03:15.01 CTV.dial CSV,avoiding: :intake @18:03:15.03 CBV.dial between AIV and waste1 @18:03:15.41 CSV.dial :air @18:03:15.94 CS.seek 10ml @18:03:42.45 CSV.dial CTV @18:03:42.99 CS.seek 0ml @18:04:09.70 CBV.dial :waste1 @18:04:10.04 delay 10 seconds @18:04:20.07 CTV.dial :air,avoiding: :intake @18:04:21.66 Storing lyfil puck in Collection Clamp to tube 3 @18:04:21.83 SC.reconfigure SCtight @18:04:21.89 SC.seek 3 @18:04:24.01 SC.reconfigure SCloose @18:04:24.22 Elbow.reconfigure TightElbow @18:04:24.28 Elbow.seek 3 @18:04:26.17 Forearm.reconfigure TightForearm @18:04:26.24 Forearm.seek 3 + 700 counts @18:04:27.31 SE.reconfigure SEpick @18:04:27.40 SE.seek beamBreak @18:04:30.51 SE.configure SEconfig @18:04:30.67 Elbow.reconfigure LimpElbow @18:04:30.73 Forearm.reconfigure LooseForearm @18:04:30.79 Forearm.seek 3 @18:04:31.09 Elbow.hold @18:04:31.17 Elbow.reconfigure TightElbow @18:04:31.27 SE.seek 0.07 Pucks below Top Plate @18:04:31.71 Forearm.force -20 @18:04:31.76 Forearm.seek 3 + 348 counts @18:04:32.09 Forearm.reconfigure TightForearm @18:04:32.15 Forearm.seek 3 + 700 counts @18:04:32.38 SE.seek 1.07 Pucks below Top Plate @18:04:33.13 Forearm.reconfigure LooseForearm @18:04:33.19 Forearm.seek :retract @18:04:34.14 Elbow.seek CC @18:04:35.78 Elbow.reconfigure LooseElbow @18:04:35.88 Forearm.seek CC @18:04:36.81 Hand.close @18:04:37.58 CC.seek :open @18:04:42.07 Forearm.seek :retract @18:04:43.03 Elbow.reconfigure TightElbow @18:04:43.09 Elbow.seek 3 @18:04:45.09 Elbow.reconfigure LimpElbow @18:04:45.15 Forearm.seek 3 @18:04:46.17 Elbow.hold @18:04:46.25 Elbow.reconfigure TightElbow @18:04:46.31 Hand.open @18:04:47.20 Forearm.force -20 @18:04:47.25 Forearm.seek 3 + 344 counts @18:04:47.55 Forearm.reconfigure TightForearm @18:04:47.61 Forearm.seek 3 + 700 counts @18:04:48.34 SE.seek :down @18:04:49.47
Forearm.reconfigure LooseForearm @18:04:49.53 Forearm.seek :retract @18:04:51.04 SE.coast @18:04:51.09
SC.coast @18:04:51.14 PSV.dial :air @18:04:52.36 PS.seek 0.499ml @18:04:58.32 delay 3 seconds @18:05:01.34 PSV.dial :mixloop @18:05:01.85 PRV.dial :air @18:05:02.36 delay 3 seconds @18:05:05.38 PRV.dial CBV @18:05:05.90 CBV.dial AIV @18:05:06.45 PRV.dial between CBV and air @18:05:06.82 PRV.dial :air @18:05:07.22 PS.reconfigure PSslow @18:05:07.28 PS.seek 0.599ml @18:05:09.79 PRV.dial :waste2 @18:05:10.62 PS.reconfigure PSconfig @18:05:10.68 PS.seek :empty @18:05:17.72 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:05:17.80 Hand.open @18:05:18.20 Elbow.seek FlushPuck::Garage @18:05:20.21 Elbow.reconfigure LooseElbow @18:05:20.31 Forearm.seek :garage @18:05:21.36 Hand.close @18:05:22.19 Forearm.seek :retract @18:05:23.24 Elbow.reconfigure TightElbow @18:05:23.30 Elbow.seek CC @18:05:26.20 Elbow.reconfigure LooseElbow @18:05:26.30 Forearm.reconfigure TightForearm @18:05:26.36 Forearm.seek CC @18:05:27.60 CC.seek :closed @18:05:31.88 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.27 @18:05:31.96 Hand.open @18:05:32.76 Forearm.reconfigure LooseForearm @18:05:32.82 Forearm.seek :retract @18:05:33.88 Clearing PML for modified lysate @18:05:33.89 CTV.dial :air,avoiding: :intake @18:05:33.92 PRV.dial :waste2 @18:05:33.94 PTV.dial :PRV,avoiding: :puck @18:05:34.79 delay 2 seconds @18:05:36.81 PTV.dial :PRVmixing,avoiding: :puck @18:05:37.63 delay 2 seconds @18:05:39.65 PSV.dial :PML @18:05:39.67 delay 2 seconds @18:05:41.69 PRV.dial :air @18:05:42.52 delay 2 seconds @18:05:44.59 PS.seek 1.499ml @18:06:01.04 delay 5 seconds @18:06:06.07 PS.seek 2.5ml @18:06:17.32 PSV.dial :air @18:06:17.84 PS.seek 0ml @18:06:44.91 mixing lysate with speDil @18:06:44.92 PSV.dial :PML @18:06:45.46 CBV.dial :PRV @18:06:45.47 PRV.dial :air @18:06:45.50 delay 2 seconds @18:06:47.53 PRV.dial :speDil @18:06:48.05 delay 2 seconds @18:06:50.14 PS.reconfigure PSslow1 @18:06:50.20 PS.seek 0.225ml @18:07:13.91 delay 5 seconds @18:07:18.93 PRV.dial CBV @18:07:19.80 delay 2 seconds @18:07:21.88 PS.seek 0.475ml @18:07:47.69 delay 5 seconds @18:07:52.71 PRV.dial :air @18:07:53.26 delay 2 seconds @18:07:55.29 PS.seek 0.965ml @18:08:45.44 delay 10 seconds @18:08:55.47 PRV.dial CBV @18:08:55.99 CTV.dial :air,avoiding: :intake @18:08:56.01 PSV.dial :air @18:08:56.55 PS.reconfigure PSconfig @18:08:56.61 PS.seek 2.5ml @18:09:13.29 delay 3 seconds @18:09:16.31 PSV.dial PTV @18:09:17.18 PTV.dial :PRV,avoiding: :puck @18:09:18.05 PS.seek 0ml @18:09:44.66 PSV.dial :air @18:09:45.52 PS.seek 2.5ml @18:10:11.94 delay 3 seconds @18:10:14.96 PSV.dial PTV @18:10:15.80 PTV.dial :PRV,avoiding: :puck @18:10:15.83 PS.seek 0ml @18:10:42.48 Pushing modified lysate into AIV Injection Loop @18:10:42.49 CBV.dial AIV @18:10:42.52 AIV.dial :bypass @18:10:42.54 PRV.dial CBV @18:10:42.57 PSV.dial :air @18:10:43.44 PS.seek 2.5ml @18:11:10.01 delay 3 seconds @18:11:13.03 PSV.dial :PML @18:11:13.54 PTV.dial :PRVmixing,avoiding: :puck @18:11:14.46 PS.reconfigure PSslow1 @18:11:14.52 PS.seek 1.74ml @18:12:31.88 delay 5 seconds @18:12:36.90 AIV.dial :sample @18:12:37.53 AWV.select :waste @18:12:37.71 PS.reconfigure PSslow2 @18:12:37.76 PS.seek 1.22ml @18:14:25.38 delay 15 seconds @18:14:40.40 AIV.dial :bypass @18:14:41.04 PS.reconfigure PSconfig @18:14:41.10 PS.seek 0ml @18:14:54.48 PSV.dial :air @18:14:55.00 PS.seek 2.5ml @18:15:21.67 PSV.dial :PML @18:15:22.21 PS.seek 0ml @18:15:48.79 AIV.dial :coilToMFB @18:15:48.80 AWV.select :air @18:15:48.98 Extracting Nucleic Acid from AIV @18:15:49.02 SPE.hold 2:46:40,55C @18:15:49.06
Beginning samplePassD @18:15:49.12 ASV.dial :SPEdelivery @18:15:49.23
AWV.select :air @18:15:49.24 AIV.dial :bypass @18:15:49.27 PSV.dial PSR @18:15:49.58 delay 20 seconds @18:15:50.57
Pulling 1.5ml of PSR.killMFB PSR.select :killMFB @18:15:50.73 PS.seek 1.5ml @18:16:07.73 delay 5 seconds @18:16:09.60 primeLysateINT @18:16:09.61 AWV.select :air @18:16:09.63 AIV.dial :coilToMFB @18:16:09.66 ATV.dial AIV @18:16:11.55 AS.reconfigure ASslow1 @18:16:11.61 AS.seek 0.299ml @18:16:12.75
PSV.dial :waste1 @18:16:13.74 PS.seek 0ml @18:16:30.79 PSV.dial PSR @18:16:31.34 PSR.select :air @18:16:31.47 PS.seek 2.5ml @18:16:59.51 PRV.dial CBV @18:16:59.55
CBV.dial PRV @18:16:59.57 PSV.dial :mixloop @18:17:00.81 CTV.dial :air,avoiding: :intake @18:17:00.82 PTV.dial :PRVmixing @18:17:00.86 PS.seek 0ml @18:17:12.32 delay 10 seconds @18:17:22.35 ATV.dial :air2 @18:17:23.25 AS.seek 0.329ml @18:17:28.89
PSV.dial PSR @18:17:29.57 ATV.dial :waste2 @18:17:30.20
Pulling 1.5ml of PSR.flush @18:17:30.21 AS.reconfigure ASnorm @18:17:30.25
PSR.select :flush @18:17:30.35 AS.seek :empty @18:17:30.45
PS.seek 1.5ml @18:17:47.41 Lysate primed! @18:17:47.42 AFV.select :holdingCoil @18:17:47.44 ATV.dial :air2 @18:17:47.64
delay 5 seconds @18:17:48.09 AS.reconfigure ASslow1 @18:17:48.15 AS.seek 0.069ml @18:17:52.66
PSV.dial :waste1 @18:17:53.22 PS.seek 0ml @18:18:02.52 delay 3 seconds @18:18:05.54 ATV.dial :AIV @18:18:06.44 AS.seek 0.469ml @18:18:10.35
PSV.dial PSR @18:18:10.90 PSR.select :air @18:18:11.03 PS.seek 2.5ml @18:18:38.98 PRV.dial CBV @18:18:39.01
CBV.dial PRV @18:18:39.04 PSV.dial :mixloop @18:18:40.26 CTV.dial :air,avoiding: :intake @18:18:40.27 PTV.dial :PRVmixing @18:18:40.31 PS.seek 0ml @18:19:08.10 PSV.dial PSR @18:19:09.49 Pulling 1.5ml of PSR.flush PSR.select :flush @18:19:09.65 PS.seek 1.5ml @18:19:26.64 delay 5 seconds @18:19:27.14 delay 5 seconds @18:19:31.66
PSV.dial :waste1 @18:19:32.23 Solid Phase Extraction Heater at 48.6C @18:19:32.25
PS.seek 0ml @18:19:32.28 ATV.dial ASV @18:19:32.74 ASV.dial :SPEdelivery @18:19:32.83 AS.seek 0.041ml @18:19:49.07
PSV.dial PSR @18:19:49.61 PSR.select :air @18:19:49.74 PS.seek 2.5ml @18:20:16.98 PRV.dial CBV @18:20:17.01
CBV.dial PRV @18:20:17.04 PSV.dial :mixloop @18:20:18.23 CTV.dial :air,avoiding: :intake @18:20:18.24 PTV.dial :PRVmixing @18:20:18.28 PS.seek 0ml @18:20:45.64 PSV.dial PSR @18:20:46.88 Pulling 1.5ml of PSR.flush PSR.select :flush @18:20:47.04 PS.seek 1.5ml @18:20:59.21 delay 10 seconds @18:21:03.54
delay 5 seconds @18:21:08.56 PSV.dial :waste1 @18:21:09.08 PS.seek 0ml @18:21:09.23 rinseLysateLineINT @18:21:09.24 AFV.select :carrierSolution @18:21:09.36 delay 2 seconds @18:21:11.38 AS.reconfigure ASconfig @18:21:11.43 AS.seek 1ml @18:21:25.76
PSV.dial PSR @18:21:26.40 PSR.select :air @18:21:26.54 PS.seek 2.5ml @18:21:53.42 PRV.dial CBV @18:21:53.83
CBV.dial PRV @18:21:53.93 PSV.dial :mixloop @18:21:55.14 CTV.dial :air,avoiding: :intake @18:21:55.15 PTV.dial :PRVmixing @18:21:55.19 PS.reconfigure PSslow @18:21:55.26 PS.seek 1.2ml @18:22:06.86 delay 1 second @18:22:07.88 AFV.select :holdingCoil @18:22:07.99 delay 1 second @18:22:09.00 ATV.dial AIV @18:22:09.57 AIV.dial :coilToMFB @18:22:09.58 AWV.select :waste @18:22:09.71 AS.reconfigure ASslow0 @18:22:09.78 AS.seek 0ml @18:22:22.11
PSV.dial :waste1 @18:22:23.65 PS.reconfigure PSconfig @18:22:23.71 PS.seek 0ml @18:22:37.01 PSV.dial :air @18:22:38.32 PS.seek 2.5ml @18:23:05.01 PSV.dial :mixloop @18:23:05.54 PS.reconfigure PSslow @18:23:05.60 PS.seek 0ml @18:23:50.66 ATV.dial :air2 @18:23:51.36 AS.reconfigure ASslow1 @18:23:51.42 AS.seek 0.05ml @18:23:56.67
PSV.dial :air @18:23:57.19 PS.reconfigure PSconfig @18:23:57.25 PS.seek 2.5ml @18:24:01.83 ATV.dial :AIV @18:24:02.69 AS.seek 0.01ml @18:24:11.02 ATV.dial :waste @18:24:12.72 AS.seek :empty @18:24:15.17 ethanol wash @18:24:15.18 ATV.dial :air2 @18:24:16.48 AS.reconfigure ASslow2 @18:24:16.54 AS.seek 0.009ml @18:24:24.08
PSV.dial :mixloop @18:24:24.60 PS.reconfigure PSslow @18:24:24.66 PS.seek 0ml @18:24:26.91 delay 3 seconds @18:24:29.94 Pulling 0.4ml of Ethanol Wash ATV.dial :ethanol @18:24:30.81 AS.reconfigure ASslow1 @18:24:30.87 AS.seek 0.409ml @18:25:15.83
Cleaning Collection Syringe @18:25:15.84 CSV.dial CSR @18:25:16.43 Pulling 4ml of CSR.flush CSR.select :flush @18:25:16.58 CS.seek 4ml @18:25:27.93 delay 5 seconds @18:25:32.95 CSR.select :air @18:25:33.09 CS.seek 10ml @18:25:49.46 CSV.dial CTV @18:25:49.49
CTV.dial CSV @18:25:51.09 CBV.dial :waste1 @18:25:51.60 delay 3 seconds @18:25:51.66
CS.seek 0ml @18:25:54.63 ATV.dial :air1 @18:25:55.03 delay 3 seconds @18:25:58.10 AS.seek 0.434ml @18:26:03.41 delay 3 seconds @18:26:06.43 ATV.dial :ethanol @18:26:07.06 AS.seek 0.419ml @18:26:10.36 delay 3 seconds @18:26:13.38 ATV.dial ASV @18:26:14.73 AS.seek 0.092ml @18:26:18.12
CSV.dial CSR @18:26:18.71 Pulling 4ml of CSR.flush CSR.select :flush @18:26:18.86 CS.seek 4ml @18:26:30.02 delay 5 seconds @18:26:35.04 CSR.select :air @18:26:35.18 CS.seek 10ml @18:26:51.56 CSV.dial CTV @18:26:51.59
CTV.dial CSV @18:26:52.12 CBV.dial :waste1 @18:26:52.14 CS.seek 0ml @18:27:18.77 Garaging FlushPuck in Collection Clamp @18:27:19.32 Elbow.reconfigure TightElbow @18:27:19.38 Elbow.seek CC @18:27:19.52 Elbow.reconfigure LooseElbow @18:27:19.63 Forearm.seek CC @18:27:20.59 Hand.close @18:27:20.74 delay 3 seconds @18:27:21.36
CC.seek :open @18:27:23.76 ATV.dial :waste2 @18:27:24.55 AS.reconfigure ASconfig @18:27:24.61 AS.seek :empty @18:27:25.77
Forearm.seek :retract @18:27:26.74 Elbow.reconfigure TightElbow @18:27:26.80 Elbow.seek :garage @18:27:29.89 Elbow.reconfigure LooseElbow @18:27:30.00 Forearm.reconfigure TightForearm @18:27:30.06 Forearm.seek :garage @18:27:30.28 ATV.dial :air2 @18:27:30.69 AS.reconfigure ASslow1 @18:27:30.75 AS.seek 1ml @18:27:31.21
Hand.open @18:27:32.04 Forearm.reconfigure LooseForearm @18:27:32.10 Forearm.seek :retract @18:27:33.12 Whole Cell Archive sampling 1200ml @18:27:33.14 Loading wcr puck from tube 4 into Collection Clamp @18:27:33.39 SC.reconfigure SCtight @18:27:33.45 SC.seek 4 @18:27:35.58 SC.reconfigure SCloose @18:27:35.80 Elbow.reconfigure TightElbow @18:27:35.87 Elbow.seek 4 @18:27:38.11 Forearm.reconfigure TightForearm @18:27:38.17 Forearm.seek 4 + 700 counts @18:27:39.19 SE.reconfigure SEpick @18:27:39.28 SE.seek beamBreak @18:27:41.98 SE.configure SEconfig @18:27:42.14 Elbow.seek 4 @18:27:42.44 Forearm.seek 4 + 300 counts @18:27:42.73 Forearm.reconfigure LooseForearm @18:27:42.79 Hand.close @18:27:43.62 SE.seek :down @18:27:43.83 Forearm.seek :retract @18:27:44.79 Elbow.seek CC @18:27:46.05 SE.coast @18:27:46.52 Elbow.reconfigure LooseElbow @18:27:46.63 Forearm.reconfigure TightForearm @18:27:46.69 Forearm.seek CC @18:27:47.94 CC.seek :closed @18:27:52.28 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.41 @18:27:52.30 Hand.open @18:27:53.18 Forearm.reconfigure LooseForearm @18:27:53.24 Forearm.seek :retract @18:27:54.24 SC.coast @18:27:54.30 collectSample 1200ml w/bubble point of 55psi @18:27:54.31 Priming 7ml @18:27:54.33 Intake.select :close @18:27:54.36 Exhaust.select :close @18:27:54.37 SS.hold @18:27:54.60 Calibrating pressure sensors @18:27:54.61 CTV.dial :air @18:27:56.18 SSV.dial :spit,avoiding: :exhaust @18:27:56.21 SS.reconfigure SSfast @18:27:56.29 SS.seek 5ml @18:27:59.22 SSV.dial CBV @18:27:59.25 CBV.dial SSV @18:28:00.87 delay 3.8 seconds @18:28:04.79 SSV.dial :spit @18:28:06.31 SS.seek 0ml @18:28:08.91 SSV.dial CBV @18:28:10.44 CTV.dial :intake @18:28:11.31 SSV.dial CBV @18:28:11.34 CBV.dial SSV @18:28:11.37 Allowing 40s for 7.0ml of remaining 7.0ml @18:28:11.40 Intake.select :open @18:28:13.59 Intake @15.9psia, vacuum from 9.4 to 13.4psi @18:28:13.61 SS.reconfigure SSinhale.with maxSpeed: 150 @18:28:13.69 SS.seek 7ml @18:28:20.18 Stroke completed -- waiting for pressure to equalize ... @18:28:44.52 Intake.select :close @18:28:44.69 SSV.dial :exhaust @18:28:45.58 Exhaust.select :open @18:28:45.71 SS.reconfigure SSexhale @18:28:45.77 SS.seek 0ml @18:28:50.19 Exhaust.select :close @18:28:50.32 Sampling 1200.0ml @18:28:50.33 Intake.select :close @18:28:50.35 Exhaust.select :close @18:28:50.38 SS.reconfigure SSconfig @18:28:50.45 SS.hold @18:28:50.66 CTV.dial :intake @18:28:50.68 SSV.dial CBV @18:28:50.72 CBV.dial SSV @18:28:51.60 Allowing 130s for 25.0ml of remaining 1200.0ml @18:28:51.62 Intake.select :open @18:28:53.81 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:28:53.83 SS.reconfigure SSinhale.with maxSpeed: 150 @18:28:53.91 SS.seek 25ml @18:28:57.57 Vacuum too high -- waiting up to 40s @18:29:06.90 Intake @15.8psia, vacuum from 9.3 to 13.3psi @18:29:06.92 SS.reconfigure SSinhale.with maxSpeed: 135 @18:29:06.99 SS.seek 25ml @18:29:10.70 Vacuum too high -- waiting up to 40s @18:29:20.46 Intake @16.0psia, vacuum from 9.5 to 13.5psi @18:29:20.48 SS.reconfigure SSinhale.with maxSpeed: 122 @18:29:20.56 SS.seek 25ml @18:29:23.81 Vacuum too high -- waiting up to 40s @18:29:32.45 Intake @16.0psia, vacuum from 9.5 to 13.5psi @18:29:32.47 SS.reconfigure SSinhale.with maxSpeed: 110 @18:29:32.55 SS.seek 25ml @18:29:36.19 Vacuum too high -- waiting up to 40s @18:29:45.76 Intake @16.0psia, vacuum from 9.5 to 13.5psi @18:29:45.78 SS.reconfigure SSinhale.with maxSpeed: 99 @18:29:45.86 SS.seek 25ml @18:29:50.29 Vacuum too high -- waiting up to 40s @18:29:59.80 Intake @15.9psia, vacuum from 9.4 to 13.4psi @18:29:59.82 SS.reconfigure SSinhale.with maxSpeed: 89 @18:29:59.90 SS.seek 25ml @18:30:01.25 Stroke completed -- waiting for pressure to equalize ... @18:30:06.67 Intake.select :close @18:30:06.84 SSV.dial :exhaust @18:30:07.72 Exhaust.select :open @18:30:07.85 SS.reconfigure SSexhale @18:30:07.91 SS.seek 0ml @18:30:17.44 Exhaust.select :close @18:30:17.56 SSV.dial CBV @18:30:17.58 CBV.dial SSV @18:30:18.49 Allowing 130s for 25.0ml of remaining 1175.0ml @18:30:18.51 Intake.select :open @18:30:20.71 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:30:20.73 SS.reconfigure SSinhale.with maxSpeed: 89 @18:30:20.81 SS.seek 25ml @18:30:24.79 Vacuum too high -- waiting up to 40s @18:30:30.85 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:30:30.87 SS.reconfigure SSinhale.with maxSpeed: 80 @18:30:30.95 SS.seek 25ml @18:30:34.26 Vacuum too high -- waiting up to 40s @18:30:40.13 Intake @15.9psia, vacuum from 9.4 to 13.4psi @18:30:40.15 SS.reconfigure SSinhale.with maxSpeed: 72 @18:30:40.22 SS.seek 25ml @18:30:43.94 Vacuum too high -- waiting up to 40s @18:30:49.78 SS.reconfigure SSinhale.with maxSpeed: 65 @18:30:49.84 SS.seek 25ml @18:30:52.35 delay 5 seconds @18:30:54.00 Vacuum too high -- waiting up to 40s @18:30:57.37 ATV.dial ASV @18:30:58.48 ASV.dial :SPEdelivery @18:30:58.51 AS.seek 0.02ml @18:30:59.95 Intake @15.8psia, vacuum from 9.3 to 13.3psi @18:30:59.97 SS.reconfigure SSinhale.with maxSpeed: 59 @18:31:00.05 SS.seek 25ml @18:31:05.29 Vacuum too high -- waiting up to 40s @18:31:11.39 Intake @15.8psia, vacuum from 9.3 to 13.3psi @18:31:11.41 SS.reconfigure SSinhale.with maxSpeed: 53 @18:31:11.49 SS.seek 25ml @18:31:17.28 Vacuum too high -- waiting up to 40s @18:31:23.63 SS.reconfigure SSinhale.with maxSpeed: 48 @18:31:23.70 SS.seek 25ml @18:31:28.03 Stroke completed -- waiting for pressure to equalize ... @18:31:36.02 Intake.select :close @18:31:36.19 SSV.dial :exhaust @18:31:37.07 Exhaust.select :open @18:31:37.20 SS.reconfigure SSexhale @18:31:37.26 SS.seek 0ml @18:31:47.10 Exhaust.select :close @18:31:47.22 SSV.dial CBV @18:31:47.24 CBV.dial SSV @18:31:48.14 Allowing 130s for 25.0ml of remaining 1150.0ml @18:31:48.16 Intake.select :open @18:31:50.35 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:31:50.37 SS.reconfigure SSinhale.with maxSpeed: 48 @18:31:50.45 SS.seek 25ml @18:31:57.73 Vacuum too high -- waiting up to 40s @18:32:02.87 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:32:02.89 SS.reconfigure SSinhale.with maxSpeed: 43 @18:32:02.97 SS.seek 25ml @18:32:11.06 Vacuum too high -- waiting up to 40s @18:32:17.18 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:32:17.20 SS.reconfigure SSinhale.with maxSpeed: 39 @18:32:17.28 SS.seek 25ml @18:32:26.97 Vacuum too high -- waiting up to 40s @18:32:32.82 SS.reconfigure SSinhale.with maxSpeed: 35 @18:32:32.89 SS.seek 25ml @18:32:47.12 Vacuum too high -- waiting up to 40s @18:32:53.91 SS.reconfigure SSinhale.with maxSpeed: 32 @18:32:53.98 SS.seek 25ml @18:33:01.85 Stroke completed -- waiting for pressure to equalize ... @18:33:09.24 Intake.select :close @18:33:09.41 SSV.dial :exhaust @18:33:10.28 Exhaust.select :open @18:33:10.41 SS.reconfigure SSexhale @18:33:10.47 SS.seek 0ml @18:33:20.16 Exhaust.select :close @18:33:20.29 SSV.dial CBV @18:33:20.31 CBV.dial SSV @18:33:21.21 Allowing 130s for 25.0ml of remaining 1125.0ml @18:33:21.23 Intake.select :open @18:33:23.42 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:33:23.44 SS.reconfigure SSinhale.with maxSpeed: 32 @18:33:23.52 SS.seek 25ml @18:33:42.44 Vacuum too high -- waiting up to 40s @18:33:48.32 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:33:48.34 SS.reconfigure SSinhale.with maxSpeed: 29 @18:33:48.42 SS.seek 25ml @18:34:15.89 delay 5 seconds @18:34:18.23 Vacuum too high -- waiting up to 40s @18:34:20.91 ATV.dial :air2 @18:34:22.08 AS.seek 0.52ml @18:34:25.35 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:34:25.37 SS.reconfigure SSinhale.with maxSpeed: 26 @18:34:25.45 SS.seek 25ml @18:34:37.17 Stroke completed -- waiting for pressure to equalize ... @18:34:45.15 Intake.select :close @18:34:45.32 SSV.dial :exhaust @18:34:46.19 Exhaust.select :open @18:34:46.32 SS.reconfigure SSexhale @18:34:46.38 SS.seek 0ml @18:34:56.00 Exhaust.select :close @18:34:56.61 SSV.dial CBV @18:34:56.63 CBV.dial SSV @18:34:57.53 Allowing 130s for 25.0ml of remaining 1100.0ml @18:34:57.55 Intake.select :open @18:34:59.74 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:34:59.76 SS.reconfigure SSinhale.with maxSpeed: 26 @18:34:59.84 SS.seek 25ml @18:35:53.82 Vacuum too high -- waiting up to 40s @18:36:00.58 SS.reconfigure SSinhale.with maxSpeed: 23 @18:36:00.65 SS.seek 25ml @18:36:02.94 delay 5 seconds @18:36:07.96 ATV.dial ASV @18:36:08.99 ASV.dial :wasteR @18:36:09.39 AS.seek 0.3ml @18:36:16.28 Stroke completed -- waiting for pressure to equalize ... @18:36:23.66 Intake.select :close @18:36:23.83 SSV.dial :exhaust @18:36:24.70 Exhaust.select :open @18:36:24.83 SS.reconfigure SSexhale @18:36:24.89 SS.seek 0ml @18:36:34.10 Exhaust.select :close @18:36:34.23 SSV.dial CBV @18:36:34.25 CBV.dial SSV @18:36:35.16 Allowing 130s for 25.0ml of remaining 1075.0ml @18:36:35.58 Intake.select :open @18:36:37.85 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:36:37.87 SS.reconfigure SSinhale.with maxSpeed: 23 @18:36:37.95 SS.seek 25ml @18:36:53.96 delay 20 seconds @18:37:14.00 AS.seek 0.07ml @18:37:54.92 Stroke completed -- waiting for pressure to equalize ... @18:38:00.54 delay 20 seconds @18:38:05.36 Intake.select :close @18:38:05.52 SSV.dial :exhaust @18:38:06.39 Exhaust.select :open @18:38:06.52 SS.reconfigure SSexhale @18:38:06.58 SS.seek 0ml @18:38:16.19 Exhaust.select :close @18:38:16.31 SSV.dial CBV @18:38:16.33 CBV.dial SSV @18:38:17.22 Allowing 130s for 25.0ml of remaining 1050.0ml @18:38:17.24 Intake.select :open @18:38:19.42 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:38:19.44 SS.reconfigure SSinhale.with maxSpeed: 23 @18:38:19.52 SS.seek 25ml @18:38:20.56 ATV.dial :waste2 @18:38:21.35 AS.reconfigure ASconfig @18:38:21.41 AS.seek :empty @18:38:25.80 Elution @18:38:25.81 ATV.dial :air1 @18:38:27.00 AS.reconfigure ASslow1 @18:38:27.06 AS.seek 0.049ml @18:38:37.46 delay 3 seconds @18:38:40.48 ATV.dial :ElutionWater @18:38:41.19 AS.seek 0.109ml @18:38:53.55 delay 3 seconds @18:38:56.57 ATV.dial ASV,via: :air2 @18:38:58.76 ASV.dial :SPEelution @18:38:59.38 AFV.select :carrierSolution @18:38:59.54 AS.reconfigure ASnorm @18:38:59.60 AS.seek 0.759ml @18:39:32.66 delay 3 seconds @18:39:35.68 AFV.select :holdingCoil @18:39:35.83 AS.reconfigure ASslow1 @18:39:35.89 AS.seek 0.474ml @18:39:36.17 Stroke completed -- waiting for pressure to equalize ... @18:39:47.10 Intake.select :close @18:39:47.26 SSV.dial :exhaust @18:39:48.14 Exhaust.select :open @18:39:48.27 SS.reconfigure SSexhale @18:39:48.33 SS.seek 0ml @18:39:58.16 Exhaust.select :close @18:39:58.28 SSV.dial CBV @18:39:58.30 CBV.dial SSV @18:39:59.20 Allowing 130s for 25.0ml of remaining 1025.0ml @18:39:59.22 Intake.select :open @18:40:01.41 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:40:01.43 SS.reconfigure SSinhale.with maxSpeed: 23 @18:40:01.51 SS.seek 25ml @18:40:33.47 delay 5 seconds @18:40:38.55 AS.reconfigure ASslow2 @18:40:38.61 AS.seek 0.439ml @18:41:14.00 delay 1:00 @18:41:17.78 Stroke completed -- waiting for pressure to equalize ... @18:41:28.23 Intake.select :close @18:41:28.40 SSV.dial :exhaust @18:41:29.27 Exhaust.select :open @18:41:29.40 SS.reconfigure SSexhale @18:41:29.47 SS.seek 0ml @18:41:38.98 Exhaust.select :close @18:41:39.11 SSV.dial CBV @18:41:39.13 CBV.dial SSV @18:41:40.03 Allowing 130s for 25.0ml of remaining 1000.0ml @18:41:40.05 Intake.select :open @18:41:42.24 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:41:42.26 SS.reconfigure SSinhale.with maxSpeed: 23 @18:41:42.34 SS.seek 25ml @18:42:14.03 pushing eluate to holding coil @18:42:14.12 AS.seek 0.279ml @18:42:58.98 Stroke completed -- waiting for pressure to equalize ... @18:43:11.11 Intake.select :close @18:43:11.27 SSV.dial :exhaust @18:43:12.14 Exhaust.select :open @18:43:12.27 SS.reconfigure SSexhale @18:43:12.33 SS.seek 0ml @18:43:22.20 Exhaust.select :close @18:43:22.32 SSV.dial CBV @18:43:22.34 CBV.dial SSV @18:43:23.22 Allowing 130s for 25.0ml of remaining 975.0ml @18:43:23.24 Intake.select :open @18:43:25.43 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:43:25.45 SS.reconfigure SSinhale.with maxSpeed: 23 @18:43:25.53 SS.seek 25ml @18:44:42.03 Stroke completed -- waiting for pressure to equalize ... @18:44:53.68 Intake.select :close @18:44:53.85 SSV.dial :exhaust @18:44:54.72 Exhaust.select :open @18:44:54.85 SS.reconfigure SSexhale @18:44:54.91 SS.seek 0ml @18:44:55.18 delay 30 seconds @18:45:04.59 Exhaust.select :close @18:45:04.71 SSV.dial CBV @18:45:04.75 CBV.dial SSV @18:45:05.61 Allowing 130s for 25.0ml of remaining 950.0ml @18:45:05.63 Intake.select :open @18:45:07.81 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:45:07.83 SS.reconfigure SSinhale.with maxSpeed: 23 @18:45:07.91 SS.seek 25ml @18:45:25.20 ATV.dial :waste,via: :air3 @18:45:27.10 AS.reconfigure ASconfig @18:45:27.16 AS.seek :empty @18:45:43.55 Parking Bleach in LysateSource line and SPEcolumn @18:45:43.56 ATV.dial :air3 @18:45:44.42 AS.reconfigure ASslow1 @18:45:44.48 AS.seek 0.029ml @18:45:50.82 ATV.dial :ElutionWater @18:45:51.44 AS.seek 0.059ml @18:45:57.74 ATV.dial :air3 @18:45:58.36 AS.seek 0.069ml @18:46:00.66 delay 2 seconds @18:46:02.68 ATV.dial :bleach @18:46:03.17 AS.seek 0.769ml @18:46:24.28 Stroke completed -- waiting for pressure to equalize ... @18:46:37.04 Intake.select :close @18:46:37.21 SSV.dial :exhaust @18:46:38.08 Exhaust.select :open @18:46:38.21 SS.reconfigure SSexhale @18:46:38.27 SS.seek 0ml @18:46:47.89 Exhaust.select :close @18:46:48.01 SSV.dial CBV @18:46:48.03 CBV.dial SSV @18:46:48.94 Allowing 130s for 25.0ml of remaining 925.0ml @18:46:48.96 Intake.select :open @18:46:51.15 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:46:51.17 SS.reconfigure SSinhale.with maxSpeed: 23 @18:46:51.25 SS.seek 25ml @18:47:40.06 Vacuum too high -- waiting up to 40s @18:47:46.82 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:47:46.84 SS.reconfigure SSinhale.with maxSpeed: 21 @18:47:46.92 SS.seek 25ml @18:48:17.94 Stroke completed -- waiting for pressure to equalize ... @18:48:24.22 delay 3 seconds @18:48:27.24 ATV.dial :air3 @18:48:27.65 Intake.select :close @18:48:27.85 SSV.dial :exhaust @18:48:27.86 delay 3 seconds @18:48:28.76 Exhaust.select :open @18:48:28.89 SS.reconfigure SSexhale @18:48:28.95 SS.seek 0ml @18:48:30.94 AS.seek 0.794ml @18:48:36.25 delay 3 seconds @18:48:38.76 Exhaust.select :close @18:48:38.88 SSV.dial CBV @18:48:38.90 CBV.dial SSV @18:48:39.27 ATV.dial :bleach @18:48:39.80 Allowing 130s for 25.0ml of remaining 900.0ml @18:48:39.82 Intake.select :open @18:48:39.97 AS.seek 0.779ml @18:48:42.09 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:48:42.11 SS.reconfigure SSinhale.with maxSpeed: 21 @18:48:42.19 SS.seek 25ml @18:48:43.30 delay 3 seconds @18:48:46.32 ATV.dial :AIV @18:48:47.64 AS.reconfigure ASconfig @18:48:47.70 AS.seek 0.015ml @18:49:31.93 ATV.dial :waste @18:49:33.47 AS.seek :empty @18:49:34.69 ATV.dial :air3 @18:49:35.39 AS.reconfigure ASslow1 @18:49:35.45 AS.seek 0.009ml @18:49:37.82 delay 2 seconds @18:49:39.84 ATV.dial :bleach @18:49:40.30 AS.seek 0.579ml @18:50:06.11 Stroke completed -- waiting for pressure to equalize ... @18:50:17.14 Intake.select :close @18:50:17.31 SSV.dial :exhaust @18:50:18.60 Exhaust.select :open @18:50:18.81 SS.reconfigure SSexhale @18:50:18.87 SS.seek 0ml @18:50:28.56 Exhaust.select :close @18:50:28.68 SSV.dial CBV @18:50:28.70 CBV.dial SSV @18:50:29.61 Allowing 130s for 25.0ml of remaining 875.0ml @18:50:29.63 Intake.select :open @18:50:31.82 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:50:31.84 SS.reconfigure SSinhale.with maxSpeed: 21 @18:50:31.92 SS.seek 25ml @18:51:35.21 delay 3 seconds @18:51:38.23 ATV.dial :air3 @18:51:38.83 delay 3 seconds @18:51:41.90 AS.seek 0.604ml @18:51:47.25 delay 3 seconds @18:51:50.27 ATV.dial :bleach @18:51:50.91 AS.seek 0.589ml @18:51:54.21 delay 3 seconds @18:51:56.00 Stroke completed -- waiting for pressure to equalize ... @18:51:57.24 ATV.dial :ASV @18:51:58.32 ASV.dial :wasteR @18:51:58.95 AS.seek 0ml @18:52:07.70 Intake.select :close @18:52:07.87 SSV.dial :exhaust @18:52:08.75 Exhaust.select :open @18:52:08.88 SS.reconfigure SSexhale @18:52:08.94 SS.seek 0ml @18:52:18.22 Exhaust.select :close @18:52:18.35 SSV.dial CBV @18:52:18.37 CBV.dial SSV @18:52:19.28 Allowing 130s for 25.0ml of remaining 850.0ml @18:52:19.30 Intake.select :open @18:52:21.49 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:52:21.51 SS.reconfigure SSinhale.with maxSpeed: 21 @18:52:21.59 SS.seek 25ml @18:53:46.17 Stroke completed -- waiting for pressure to equalize ... @18:53:57.69 delay 1:00 @18:53:59.53 Intake.select :close @18:53:59.69 SSV.dial :exhaust @18:54:00.56 Exhaust.select :open @18:54:00.69 SS.reconfigure SSexhale @18:54:00.75 SS.seek 0ml @18:54:10.58 Exhaust.select :close @18:54:10.70 SSV.dial CBV @18:54:10.72 CBV.dial SSV @18:54:11.61 Allowing 130s for 25.0ml of remaining 825.0ml @18:54:11.63 Intake.select :open @18:54:13.82 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:54:13.84 SS.reconfigure SSinhale.with maxSpeed: 21 @18:54:13.92 SS.seek 25ml @18:54:57.71 AFV.select :carrierSolution @18:54:57.83 delay 2 seconds @18:54:59.85 AS.reconfigure ASconfig @18:54:59.90 AS.seek 1ml @18:55:37.62 Stroke completed -- waiting for pressure to equalize ... @18:55:50.50 Intake.select :close @18:55:50.67 SSV.dial :exhaust @18:55:51.54 Exhaust.select :open @18:55:51.66 SS.reconfigure SSexhale @18:55:51.72 SS.seek 0ml @18:55:57.66 delay 1 second @18:55:58.68 AFV.select :holdingCoil @18:55:58.79 delay 1 second @18:55:59.80 ATV.dial ASV @18:55:59.81 ASV.dial :wasteR @18:55:59.84 AS.reconfigure ASslow1 @18:55:59.92 AS.seek 0ml @18:56:01.42 Exhaust.select :close @18:56:01.54 SSV.dial CBV @18:56:01.56 CBV.dial SSV @18:56:02.47 Allowing 130s for 25.0ml of remaining 800.0ml @18:56:02.49 Intake.select :open @18:56:04.68 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:56:04.70 SS.reconfigure SSinhale.with maxSpeed: 21 @18:56:04.78 SS.seek 25ml @18:57:15.77 Vacuum too high -- waiting up to 40s @18:57:23.05 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:57:23.07 SS.reconfigure SSinhale.with maxSpeed: 19 @18:57:23.15 SS.seek 25ml @18:57:37.89 Stroke completed -- waiting for pressure to equalize ... @18:57:46.48 Intake.select :close @18:57:46.65 SSV.dial :exhaust @18:57:47.53 Exhaust.select :open @18:57:47.65 SS.reconfigure SSexhale @18:57:47.71 SS.seek 0ml @18:57:57.57 Exhaust.select :close @18:57:57.70 SSV.dial CBV @18:57:57.72 CBV.dial SSV @18:57:58.62 Allowing 130s for 25.0ml of remaining 775.0ml @18:57:58.64 Intake.select :open @18:58:00.83 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:58:00.85 SS.reconfigure SSinhale.with maxSpeed: 19 @18:58:00.93 SS.seek 25ml @18:59:21.33 delay 20 seconds @18:59:33.82 Stroke completed -- waiting for pressure to equalize ... @18:59:41.35 ATV.dial :air3 @18:59:42.96 AS.seek 0.59ml @18:59:44.76 Intake.select :close @18:59:44.93 SSV.dial :exhaust @18:59:45.82 Exhaust.select :open @18:59:45.95 SS.reconfigure SSexhale @18:59:46.01 SS.seek 0ml @18:59:55.64 Exhaust.select :close @18:59:55.76 SSV.dial CBV @18:59:55.78 CBV.dial SSV @18:59:56.67 Allowing 130s for 25.0ml of remaining 750.0ml @18:59:56.69 Intake.select :open @18:59:58.88 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:59:58.90 SS.reconfigure SSinhale.with maxSpeed: 19 @18:59:58.98 SS.seek 25ml @19:01:32.07 Stroke completed -- waiting for pressure to equalize ... @19:01:41.93 delay 3 seconds @19:01:44.35 Intake.select :close @19:01:44.52 SSV.dial :exhaust @19:01:44.95 ATV.dial :ASV @19:01:45.39 Exhaust.select :open @19:01:45.52 SS.reconfigure SSexhale @19:01:45.58 SS.seek 0ml @19:01:46.40 ASV.dial :wasteR @19:01:46.49 AS.seek 0.09ml @19:01:55.32 Exhaust.select :close @19:01:55.44 SSV.dial CBV @19:01:55.46 CBV.dial SSV @19:01:56.35 Allowing 130s for 25.0ml of remaining 725.0ml @19:01:56.37 Intake.select :open @19:01:58.56 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:01:58.58 SS.reconfigure SSinhale.with maxSpeed: 19 @19:01:58.66 SS.seek 25ml @19:03:27.33 delay 30 seconds @19:03:31.39 Stroke completed -- waiting for pressure to equalize ... @19:03:43.54 Intake.select :close @19:03:43.71 SSV.dial :exhaust @19:03:44.59 Exhaust.select :open @19:03:44.72 SS.reconfigure SSexhale @19:03:44.78 SS.seek 0ml @19:03:54.46 Exhaust.select :close @19:03:54.58 SSV.dial CBV @19:03:54.60 CBV.dial SSV @19:03:55.51 Allowing 130s for 25.0ml of remaining 700.0ml @19:03:55.53 Intake.select :open @19:03:57.35 ATV.dial :extSrc @19:03:57.72 Intake @15.7psia, vacuum from 9.2 to 13.2psi @19:03:57.74 SS.reconfigure SSinhale.with maxSpeed: 19 @19:03:57.82 SS.seek 25ml @19:03:58.26 delay 3 seconds @19:04:01.28 ATV.dial :ElutionWater @19:04:02.46 AS.seek 0.12ml @19:04:08.71 ATV.dial :air3 @19:04:09.35 AS.seek 0.13ml @19:04:11.66 delay 2 seconds @19:04:13.68 ATV.dial :bleach @19:04:14.14 AS.seek 0.23ml @19:04:34.55 delay 3 seconds @19:04:37.57 ATV.dial :air3 @19:04:38.15 delay 3 seconds @19:04:41.22 AS.seek 0.255ml @19:04:46.58 delay 3 seconds @19:04:49.60 ATV.dial :bleach @19:04:50.24 AS.seek 0.24ml @19:04:53.54 delay 3 seconds @19:04:56.56 ATV.dial :ASV @19:04:57.63 ASV.dial :SPEdelivery @19:04:58.04 AFV.select :carrierSolution @19:04:58.16 delay 2 seconds @19:05:00.18 AS.reconfigure ASconfig @19:05:00.24 AS.seek 0.5ml @19:05:15.49 delay 1 second @19:05:16.51 AFV.select :holdingCoil @19:05:16.66 AS.reconfigure ASslow1 @19:05:16.72 AS.seek 0.17ml @19:05:31.15 Stroke completed -- waiting for pressure to equalize ... @19:05:43.27 Intake.select :close @19:05:43.44 SSV.dial :exhaust @19:05:44.32 Exhaust.select :open @19:05:44.45 SS.reconfigure SSexhale @19:05:44.51 SS.seek 0ml @19:05:54.26 Exhaust.select :close @19:05:54.38 SSV.dial CBV @19:05:54.40 CBV.dial SSV @19:05:55.29 Allowing 130s for 25.0ml of remaining 675.0ml @19:05:55.31 Intake.select :open @19:05:57.49 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:05:57.51 SS.reconfigure SSinhale.with maxSpeed: 19 @19:05:57.59 SS.seek 25ml @19:06:23.37 SPE.hold 2:46:40,95C @19:06:23.41 delay 3:00 @19:07:30.42 Stroke completed -- waiting for pressure to equalize ... @19:07:43.30 Intake.select :close @19:07:43.47 SSV.dial :exhaust @19:07:44.37 Exhaust.select :open @19:07:44.50 SS.reconfigure SSexhale @19:07:44.56 SS.seek 0ml @19:07:54.45 Exhaust.select :close @19:07:54.58 SSV.dial CBV @19:07:54.60 CBV.dial SSV @19:07:55.54 Allowing 130s for 25.0ml of remaining 650.0ml @19:07:55.56 Intake.select :open @19:07:57.75 Intake @15.7psia, vacuum from 9.2 to 13.2psi @19:07:57.77 SS.reconfigure SSinhale.with maxSpeed: 19 @19:07:57.85 SS.seek 25ml @19:09:23.44 AFV.select :carrierSolution @19:09:23.56 delay 2 seconds @19:09:25.58 AS.reconfigure ASconfig @19:09:25.63 AS.seek 1ml @19:09:30.56 Stroke completed -- waiting for pressure to equalize ... @19:09:43.34 Intake.select :close @19:09:43.50 SSV.dial :exhaust @19:09:44.40 Exhaust.select :open @19:09:44.53 SS.reconfigure SSexhale @19:09:44.59 SS.seek 0ml @19:09:54.65 Exhaust.select :close @19:09:54.77 SSV.dial CBV @19:09:54.79 CBV.dial SSV @19:09:55.73 Allowing 130s for 25.0ml of remaining 625.0ml @19:09:55.75 Intake.select :open @19:09:57.93 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:09:57.95 SS.reconfigure SSinhale.with maxSpeed: 19 @19:09:58.03 SS.seek 25ml @19:10:13.65 delay 1 second @19:10:14.67 AFV.select :holdingCoil @19:10:14.78 delay 1 second @19:10:15.79 ASV.dial :wasteD @19:10:16.91 AS.seek 0ml @19:10:53.77 Vacuum too high -- waiting up to 40s @19:10:59.15 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:10:59.18 SS.reconfigure SSinhale.with maxSpeed: 17 @19:10:59.25 SS.seek 25ml @19:11:14.69 Priming Eluate @19:11:14.70 AFV.select :carrierSolution @19:11:14.83 delay 2 seconds @19:11:16.85 AS.seek 0.5ml @19:11:41.32 Stroke completed -- waiting for pressure to equalize ... @19:11:45.88 delay 1 second @19:11:46.89 AFV.select :holdingCoil @19:11:46.99 ATV.dial ASV @19:11:47.01 ASV.dial :SPErecovery @19:11:47.95 Swish #1 of 2 @19:11:48.04 AS.reconfigure ASslow0 @19:11:48.10 AS.seek 0.6ml @19:11:50.44 Intake.select :close @19:11:50.61 SSV.dial :exhaust @19:11:51.51 Exhaust.select :open @19:11:51.64 SS.reconfigure SSexhale @19:11:51.70 SS.seek 0ml @19:11:58.49 AS.seek 0.5ml @19:12:01.52 Exhaust.select :close @19:12:01.64 SSV.dial CBV @19:12:01.66 CBV.dial SSV @19:12:02.57 Allowing 130s for 25.0ml of remaining 600.0ml @19:12:02.59 Intake.select :open @19:12:04.78 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:12:04.80 SS.reconfigure SSinhale.with maxSpeed: 17 @19:12:04.88 SS.seek 25ml @19:12:08.87 Swish #2 of 2 @19:12:08.96 AS.seek 0.6ml @19:12:19.46 AS.seek 0.5ml @19:12:29.80 delay 3 seconds @19:12:32.87 AS.reconfigure ASslow1 @19:12:32.93 AS.seek 0.684ml @19:13:10.29 delay 3 seconds @19:13:13.32 Eluate primed ATV.dial :waste2 @19:13:14.00 AS.reconfigure ASnorm @19:13:14.06 AS.seek 0ml @19:13:48.88 SPE.coast @19:13:48.98 Stroke completed -- waiting for pressure to equalize ... @19:13:49.00 ASV.dial :SPErecovery @19:13:49.03 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118nov2419h1200ml.pcr @19:13:49.06 Building PCR Slug from ASV with MM2 mix, PP4 primer @19:13:49.09 Priming with carrier @19:13:49.10 ATV.dial ARV @19:13:50.23 ARV.dial :carrier @19:13:50.61 AS.seek 0.5ml @19:14:00.04 Intake.select :close @19:14:00.21 SSV.dial :exhaust @19:14:01.09 Exhaust.select :open @19:14:01.21 SS.reconfigure SSexhale @19:14:01.28 SS.seek 0ml @19:14:10.90 Exhaust.select :close @19:14:11.02 SSV.dial CBV @19:14:11.07 CBV.dial SSV @19:14:11.90 Allowing 130s for 25.0ml of remaining 575.0ml @19:14:11.92 Intake.select :open @19:14:14.11 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:14:14.13 SS.reconfigure SSinhale.with maxSpeed: 17 @19:14:14.21 SS.seek 25ml @19:14:16.19 AFV.select :carrierSolution @19:14:16.73 delay 2 seconds @19:14:18.78 AS.reconfigure ASconfig @19:14:18.84 AS.seek 1ml @19:14:47.87 delay 1 second @19:14:48.89 AFV.select :holdingCoil @19:14:49.00 delay 1 second @19:14:50.01 ATV.dial :waste @19:14:51.58 AS.reconfigure ASnorm @19:14:51.64 AS.seek :empty @19:15:42.41 AFV.select :carrierSolution @19:15:42.53 delay 2 seconds @19:15:44.55 AS.reconfigure ASconfig @19:15:44.62 AS.seek 0.5ml @19:15:58.40 Stroke completed -- waiting for pressure to equalize ... @19:16:10.67 Intake.select :close @19:16:10.84 SSV.dial :exhaust @19:16:11.70 Exhaust.select :open @19:16:11.83 SS.reconfigure SSexhale @19:16:11.89 SS.seek 0ml @19:16:13.74 delay 1 second @19:16:14.76 AFV.select :holdingCoil @19:16:14.90 Building Slug @19:16:14.91 ATV.dial :ARV @19:16:16.58 delay 2 seconds @19:16:18.60 ARV.dial :air1 @19:16:19.02 delay 2 seconds @19:16:21.09 AS.reconfigure ASslow2 @19:16:21.16 AS.seek 0.506ml @19:16:21.48 Exhaust.select :close @19:16:21.60 SSV.dial CBV @19:16:21.62 CBV.dial SSV @19:16:22.49 Allowing 130s for 25.0ml of remaining 550.0ml @19:16:22.51 Intake.select :open @19:16:24.70 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:16:24.72 SS.reconfigure SSinhale.with maxSpeed: 17 @19:16:24.80 SS.seek 25ml @19:16:27.40 delay 2 seconds @19:16:29.42 ARV.dial :MM2 @19:16:31.00 delay 2 seconds @19:16:33.02 Adding MM2 @19:16:33.11 AS.seek 0.524ml @19:16:51.35 delay 25 seconds @19:17:16.37 ARV.dial :PP4 @19:17:16.79 Adding PP4 @19:17:16.81 delay 2 seconds @19:17:18.89 AS.seek 0.53ml @19:17:25.16 delay 10 seconds @19:17:35.19 ARV.dial :air3 @19:17:35.58 adding ARV air @19:17:35.60 delay 2 seconds @19:17:37.68 AS.seek 0.536ml @19:17:44.13 delay 5 seconds @19:17:49.15 ARV.dial :carrier @19:17:50.51 delay 7 seconds @19:17:57.60 AS.seek 0.594ml @19:18:08.94 Stroke completed -- waiting for pressure to equalize ... @19:18:21.71 Intake.select :close @19:18:21.88 SSV.dial :exhaust @19:18:22.76 Exhaust.select :open @19:18:22.89 SS.reconfigure SSexhale @19:18:22.95 SS.seek 0ml @19:18:32.69 Exhaust.select :close @19:18:32.81 SSV.dial CBV @19:18:32.86 CBV.dial SSV @19:18:33.69 Allowing 130s for 25.0ml of remaining 525.0ml @19:18:33.71 Intake.select :open @19:18:35.91 Intake @15.7psia, vacuum from 9.2 to 13.2psi @19:18:35.93 SS.reconfigure SSinhale.with maxSpeed: 17 @19:18:36.01 SS.seek 25ml @19:18:56.05 delay 5 seconds @19:19:01.07 ATV.dial :ASV @19:19:01.49 delay 2 seconds @19:19:03.57 AS.seek 0.6ml @19:19:09.82 delay 4 seconds @19:19:13.84 ATV.dial ARV @19:19:14.41 delay 2 seconds @19:19:16.43 ARV.dial :carrier @19:19:16.45 delay 2 seconds @19:19:18.53 AS.seek 0.65ml @19:20:08.97 delay 4 seconds @19:20:12.99 ATV.dial PCR @19:20:13.62 ASV.dial :waste1 @19:20:14.43 ARV.dial :air1 @19:20:14.83 Positioning Slug by 0.324ml @19:20:14.93 AS.seek 0.326ml @19:20:20.20 Stroke completed -- waiting for pressure to equalize ... @19:20:32.98 Intake.select :close @19:20:33.14 SSV.dial :exhaust @19:20:33.99 Exhaust.select :open @19:20:34.12 SS.reconfigure SSexhale @19:20:34.18 SS.seek 0ml @19:20:44.12 Exhaust.select :close @19:20:44.24 SSV.dial CBV @19:20:44.26 CBV.dial SSV @19:20:45.15 Allowing 130s for 25.0ml of remaining 500.0ml @19:20:45.17 Intake.select :open @19:20:47.36 Intake @15.7psia, vacuum from 9.2 to 13.2psi @19:20:47.38 SS.reconfigure SSinhale.with maxSpeed: 17 @19:20:47.46 SS.seek 25ml @19:22:32.05 Stroke completed -- waiting for pressure to equalize ... @19:22:44.32 Intake.select :close @19:22:44.49 SSV.dial :exhaust @19:22:45.37 Exhaust.select :open @19:22:45.50 SS.reconfigure SSexhale @19:22:45.56 SS.seek 0ml @19:22:54.87 Exhaust.select :close @19:22:54.99 SSV.dial CBV @19:22:55.01 CBV.dial SSV @19:22:55.91 Allowing 130s for 25.0ml of remaining 475.0ml @19:22:55.93 Intake.select :open @19:22:58.12 Intake @15.7psia, vacuum from 9.2 to 13.2psi @19:22:58.14 SS.reconfigure SSinhale.with maxSpeed: 17 @19:22:58.22 SS.seek 25ml @19:24:43.44 Stroke completed -- waiting for pressure to equalize ... @19:24:57.45 Intake.select :close @19:24:57.62 SSV.dial :exhaust @19:24:58.49 Exhaust.select :open @19:24:58.62 SS.reconfigure SSexhale @19:24:58.68 SS.seek 0ml @19:25:08.41 Exhaust.select :close @19:25:08.53 SSV.dial CBV @19:25:08.55 CBV.dial SSV @19:25:09.44 Allowing 130s for 25.0ml of remaining 450.0ml @19:25:09.46 Intake.select :open @19:25:11.64 Intake @15.7psia, vacuum from 9.2 to 13.2psi @19:25:11.66 SS.reconfigure SSinhale.with maxSpeed: 17 @19:25:11.74 SS.seek 25ml @19:25:41.08 delay 5 seconds @19:25:46.10 ATV.dial ARV @19:25:46.90 ASV.dial :PCRlock @19:25:47.30 delay 5 seconds @19:25:52.33 Running Tt1 Profile60C300 thermocycling @19:25:52.34 PCR.program Profile60C300 @19:25:52.49 Profile60C300 should complete in < 1:45:21 @19:25:52.50 PCR.run 2:16:41.25 @19:26:56.10 Stroke completed -- waiting for pressure to equalize ... @19:27:10.19 Intake.select :close @19:27:10.36 SSV.dial :exhaust @19:27:11.22 Exhaust.select :open @19:27:11.37 SS.reconfigure SSexhale @19:27:11.43 SS.seek 0ml @19:27:21.08 Exhaust.select :close @19:27:21.20 SSV.dial CBV @19:27:21.22 CBV.dial SSV @19:27:22.13 Allowing 130s for 25.0ml of remaining 425.0ml @19:27:22.15 Intake.select :open @19:27:24.34 Intake @15.7psia, vacuum from 9.2 to 13.2psi @19:27:24.36 SS.reconfigure SSinhale.with maxSpeed: 17 @19:27:24.44 SS.seek 25ml @19:29:00.03 Vacuum too high -- waiting up to 40s @19:29:07.26 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:29:07.28 SS.reconfigure SSinhale.with maxSpeed: 15 @19:29:07.36 SS.seek 25ml @19:29:17.66 Stroke completed -- waiting for pressure to equalize ... @19:29:26.27 Intake.select :close @19:29:26.44 SSV.dial :exhaust @19:29:27.32 Exhaust.select :open @19:29:27.45 SS.reconfigure SSexhale @19:29:27.51 SS.seek 0ml @19:29:37.41 Exhaust.select :close @19:29:37.53 SSV.dial CBV @19:29:37.55 CBV.dial SSV @19:29:38.54 Allowing 130s for 25.0ml of remaining 400.0ml @19:29:38.56 Intake.select :open @19:29:40.75 Intake @15.7psia, vacuum from 9.2 to 13.2psi @19:29:40.77 SS.reconfigure SSinhale.with maxSpeed: 15 @19:29:40.85 SS.seek 25ml @19:31:38.94 Stroke completed -- waiting for pressure to equalize ... @19:31:48.57 SS.reconfigure SSconfig @19:31:48.63 SS.hold @19:31:48.76 Intake.select :close @19:31:48.92 ShallowSampler::Clogged in WCR -- progress too slow after 825.0ml @19:31:48.94 SSV.dial :exhaust @19:31:49.82 Exhaust.select :open @19:31:49.95 SS.reconfigure SSexhale @19:31:50.01 SS.seek 0ml @19:31:59.91 Exhaust.select :close @19:32:00.03 Sampled 825.0ml @19:32:00.05 Drying puck before fixing for 4:00 @19:32:00.06 CBV.dial :waste2b @19:32:00.97 delay 1:00 @19:33:00.99 CSV.dial :air @19:33:01.51 CS.reconfigure CSfast @19:33:01.57 CS.seek 10ml @19:33:28.32 Pumping wcr puck from tube 4 up to 55psi... @19:33:28.34 CBV.dial SSV @19:33:29.78 CBV.dial between CSV and waste2b @19:33:30.89 CSV.dial between air and CTV @19:33:31.27 CS.seek 7.206ml @19:33:39.54 CTV.dial CSV,avoiding: :air @19:33:39.57 CSV.dial CTV @19:33:40.48 CS.reconfigure CSpressurize @19:33:40.54 CS.seek 0ml @19:33:59.91 CSV.dial :air @19:34:00.45 CS.reconfigure CSfast @19:34:00.51 CS.seek 10ml @19:34:27.38 CSV.dial between air and CTV @19:34:27.77 CS.seek 2.041ml @19:34:49.22 CTV.dial CSV,avoiding: :air @19:34:49.25 CSV.dial CTV @19:34:49.61 CS.reconfigure CSpressurize @19:34:49.68 CS.seek 0ml @19:34:50.75 CBV.dial :waste2b @19:34:51.11 CS.reconfigure CSconfig @19:34:51.18 CS.seek :empty @19:34:57.04 delay 1:00 @19:35:57.06 CTV.dial :air,avoiding: :intake @19:35:58.61 Fix #1 of 2 (with rnal) for 2:00 @19:35:58.62 CSV.dial CSR @19:35:59.22 Pulling 2ml of CSR.rnal @19:35:59.23 CSR.select :rnal @19:35:59.38 CS.seek 2ml @19:36:05.79 delay 5 seconds @19:36:10.81 CSR.select :air @19:36:10.95 CS.reconfigure CSfast @19:36:11.01 CS.seek 10ml @19:36:32.57 Pumping wcr puck from tube 4 up to 55psi... @19:36:32.59 CBV.dial SSV @19:36:34.04 CBV.dial between CSV and waste2b @19:36:35.16 CSV.dial between air and CTV @19:36:35.88 CS.seek 7.219ml @19:36:44.01 CTV.dial CSV,avoiding: :air @19:36:44.09 CSV.dial CTV @19:36:45.61 CS.reconfigure CSpressurize @19:36:45.68 CS.seek 0ml @19:37:04.85 CSV.dial :air @19:37:05.39 CS.reconfigure CSfast @19:37:05.45 CS.seek 10ml @19:37:32.04 CSV.dial between air and CTV @19:37:32.42 CS.seek 2.036ml @19:37:54.10 CTV.dial CSV,avoiding: :air @19:37:54.13 CSV.dial CTV @19:37:54.50 CS.reconfigure CSpressurize @19:37:54.57 CS.seek 0ml @19:37:55.59 delay 2:00 @19:39:55.61 CBV.dial :waste2b @19:39:55.99 CS.reconfigure CSconfig @19:39:56.05 CS.seek :empty @19:40:02.04 delay 1:00 @19:41:02.06 CTV.dial :air,avoiding: :intake @19:41:03.61 Fix #2 of 2 (with rnal) for 2:00 @19:41:03.62 CSV.dial CSR @19:41:04.21 Pulling 2ml of CSR.rnal @19:41:04.22 CSR.select :rnal @19:41:04.37 CS.seek 2ml @19:41:10.54 delay 5 seconds @19:41:15.56 CSR.select :air @19:41:15.70 CS.reconfigure CSfast @19:41:15.76 CS.seek 10ml @19:41:37.51 Pumping wcr puck from tube 4 up to 55psi... @19:41:37.52 CBV.dial SSV @19:41:38.96 CBV.dial between CSV and waste2b @19:41:40.07 CSV.dial between air and CTV @19:41:40.79 CS.seek 7.193ml @19:41:49.04 CTV.dial CSV,avoiding: :air @19:41:49.07 CSV.dial CTV @19:41:50.66 CS.reconfigure CSpressurize @19:41:50.72 CS.seek 0ml @19:42:09.82 CSV.dial :air @19:42:10.36 CS.reconfigure CSfast @19:42:10.42 CS.seek 10ml @19:42:37.04 CSV.dial between air and CTV @19:42:37.41 CS.seek 2.033ml @19:42:58.86 CTV.dial CSV,avoiding: :air @19:42:58.90 CSV.dial CTV @19:42:59.26 CS.reconfigure CSpressurize @19:42:59.32 CS.seek 0ml @19:43:00.28 delay 2:00 @19:45:00.30 CBV.dial :waste2b @19:45:00.67 CS.reconfigure CSconfig @19:45:00.74 CS.seek :empty @19:45:06.83 delay 1:00 @19:46:06.85 CTV.dial :air,avoiding: :intake @19:46:08.96 Storing wcr puck in Collection Clamp to tube 3 @19:46:09.16 SC.reconfigure SCtight @19:46:09.23 SC.seek 3 @19:46:11.35 SC.reconfigure SCloose @19:46:11.57 Elbow.reconfigure TightElbow @19:46:11.63 Elbow.seek 3 @19:46:13.66 Forearm.reconfigure TightForearm @19:46:13.72 Forearm.seek 3 + 700 counts @19:46:14.85 SE.reconfigure SEpick @19:46:14.94 SE.seek beamBreak @19:46:18.10 SE.configure SEconfig @19:46:18.29 Elbow.reconfigure LimpElbow @19:46:18.35 Forearm.reconfigure LooseForearm @19:46:18.42 Forearm.seek 3 @19:46:18.72 Elbow.hold @19:46:18.81 Elbow.reconfigure TightElbow @19:46:18.92 SE.seek 1.08 Pucks below Top Plate @19:46:19.43 Forearm.force -20 @19:46:19.48 Forearm.seek 3 + 347 counts @19:46:19.80 Forearm.reconfigure TightForearm @19:46:19.87 Forearm.seek 3 + 700 counts @19:46:20.11 SE.seek 2.08 Pucks below Top Plate @19:46:20.69 Forearm.reconfigure LooseForearm @19:46:20.76 Forearm.seek :retract @19:46:21.72 Elbow.seek CC @19:46:23.46 Elbow.reconfigure LooseElbow @19:46:23.57 Forearm.seek CC @19:46:24.56 Hand.close @19:46:25.33 CC.seek :open @19:46:29.84 Forearm.seek :retract @19:46:30.81 Elbow.reconfigure TightElbow @19:46:30.88 Elbow.seek 3 @19:46:32.84 Elbow.reconfigure LimpElbow @19:46:32.91 Forearm.seek 3 @19:46:33.90 Elbow.hold @19:46:33.99 Elbow.reconfigure TightElbow @19:46:34.05 Hand.open @19:46:34.94 Forearm.force -20 @19:46:35.00 Forearm.seek 3 + 350 counts @19:46:35.30 Forearm.reconfigure TightForearm @19:46:35.36 Forearm.seek 3 + 700 counts @19:46:36.33 SE.seek :down @19:46:37.46 Forearm.reconfigure LooseForearm @19:46:37.52 Forearm.seek :retract @19:46:39.04 SE.coast @19:46:39.08 SC.coast @19:46:39.15 Starting finalPurge @19:46:39.17 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:46:39.27 Hand.open @19:46:39.69 Elbow.seek FlushPuck::Garage @19:46:41.74 Elbow.reconfigure LooseElbow @19:46:41.85 Forearm.seek :garage @19:46:42.87 Hand.close @19:46:43.69 Forearm.seek :retract @19:46:44.70 Elbow.reconfigure TightElbow @19:46:44.76 Elbow.seek CC @19:46:47.70 Elbow.reconfigure LooseElbow @19:46:47.81 Forearm.reconfigure TightForearm @19:46:47.87 Forearm.seek CC @19:46:49.14 CC.seek :closed @19:46:53.37 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.30 @19:46:53.47 Hand.open @19:46:54.26 Forearm.reconfigure LooseForearm @19:46:54.32 Forearm.seek :retract @19:46:55.28 Flushing the Collection Valve::Manifold @19:46:55.42 CSV.dial CSR @19:46:56.00 Pulling 5ml of CSR.flush CSR.select :flush @19:46:56.16 CS.seek 5ml @19:47:10.01 delay 5 seconds @19:47:15.03 CSR.select :air @19:47:15.21 CS.reconfigure CSfast @19:47:15.27 CS.seek 7.5ml @19:47:22.67 CSV.dial :waste @19:47:24.19 CS.reconfigure CSconfig @19:47:24.26 CS.seek 0ml @19:47:44.48 Flushing lower manifold's waste1 port @19:47:44.62 CSV.dial :flush @19:47:45.15 CS.reconfigure CSfast @19:47:45.21 CS.seek 5ml @19:47:59.05 delay 5 seconds @19:48:04.07 CSV.dial :air @19:48:04.78 CS.seek 7.001ml @19:48:10.83 CSV.dial CTV @19:48:10.85
CTV.dial CSV @19:48:12.94 CBV.dial :waste1 @19:48:13.85 CS.seek 0ml @19:48:32.64 Flushing lower manifold's waste2b port @19:48:32.79 CSV.dial :flush @19:48:33.67 CS.seek 5ml @19:48:47.61 delay 5 seconds @19:48:52.63 CSV.dial :air @19:48:53.24 CS.seek 7ml @19:48:59.52 CSV.dial CTV @19:48:59.55
CTV.dial CSV @19:49:00.08 CBV.dial :waste2b @19:49:00.96 CS.seek 0ml @19:49:19.73 Flushing lower manifold's waste1 port @19:49:19.74 CSV.dial CSR @19:49:20.33 Pulling 4ml of CSR.mfbkill @19:49:20.34 CSR.select :mfbkill @19:49:20.48 CS.reconfigure CSconfig @19:49:20.54 CS.seek 4ml @19:49:31.86 delay 5 seconds @19:49:36.88 CSR.select :air @19:49:37.01 CS.reconfigure CSfast @19:49:37.08 CS.seek 10ml @19:49:53.33 CSV.dial CTV @19:49:53.36
CTV.dial CSV @19:49:53.89 CBV.dial :waste1 @19:49:54.78 CS.seek 0ml @19:50:21.20 Flushing lower manifold's waste2b port @19:50:21.21 CSV.dial CSR @19:50:21.81 Pulling 4ml of CSR.mfbkill @19:50:21.82 CSR.select :mfbkill @19:50:21.96 CS.reconfigure CSconfig @19:50:22.02 CS.seek 4ml @19:50:33.20 delay 5 seconds @19:50:38.22 CSR.select :air @19:50:38.36 CS.reconfigure CSfast @19:50:38.43 CS.seek 10ml @19:50:54.92 CSV.dial CTV @19:50:54.96
CTV.dial CSV @19:50:55.50 CBV.dial :waste2b @19:50:56.39 CS.seek 0ml @19:51:22.86 Killing upper manifold @19:51:22.87 CSV.dial CSR @19:51:23.47 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @19:51:23.61 CS.reconfigure CSconfig @19:51:23.68 CS.seek 4ml @19:51:34.86 delay 5 seconds @19:51:39.88 CSR.select :air @19:51:40.02 CS.reconfigure CSfast @19:51:40.09 CS.seek 10ml @19:51:56.30 CSV.dial CBV @19:51:56.32
CBV.dial CSV @19:51:57.56 CTV.dial :air @19:51:59.09 CS.seek 0ml @19:52:25.49 delay 7 seconds @19:52:32.52 CS.seek 3ml @19:52:41.24 CSV.dial :waste @19:52:41.76 CS.seek 0ml @19:52:50.58 Flushing upper manifold @19:52:51.22 CSV.dial :flush @19:52:51.75 CS.seek 5ml @19:53:05.49 delay 5 seconds @19:53:10.51 CSV.dial :air @19:53:11.09 CS.seek 7ml @19:53:17.17 CSV.dial CBV @19:53:17.20
CBV.dial CSV @19:53:18.39 CTV.dial :air @19:53:18.42 CS.seek 0ml @19:53:37.08 delay 7 seconds @19:53:44.11 CS.seek 3ml @19:53:52.71 CSV.dial :waste @19:53:53.23 CS.seek 0ml @19:54:02.13 CSV.dial :flush @19:54:02.65 CS.seek 3ml @19:54:11.27 delay 5 seconds @19:54:16.29 CSV.dial :air @19:54:16.88 CS.seek 5ml @19:54:23.21 CSV.dial :air @19:54:23.23 CS.seek 0ml @19:54:37.08 delay 7 seconds @19:54:44.11 CS.seek 3ml @19:54:52.77 CSV.dial :waste @19:54:53.64 CS.seek 0ml @19:55:02.27 Flushing lower manifold's waste1 port @19:55:02.41 CSV.dial :flush @19:55:02.94 CS.seek 5ml @19:55:16.61 delay 5 seconds @19:55:21.63 CSV.dial :air @19:55:22.23 CS.seek 7ml @19:55:28.27 CSV.dial CTV @19:55:28.30
CTV.dial CSV @19:55:29.91 CBV.dial :waste1 @19:55:30.44 CS.seek 0ml @19:55:49.21 Flushing lower manifold's waste2b port @19:55:49.37 CSV.dial :flush @19:55:50.25 CS.seek 5ml @19:56:03.89 delay 5 seconds @19:56:08.91 CSV.dial :air @19:56:09.52 CS.seek 7ml @19:56:15.55 CSV.dial CTV @19:56:15.58
CTV.dial CSV @19:56:16.11 CBV.dial :waste2b @19:56:17.00 CS.seek 0ml @19:56:35.77 Garaging FlushPuck in Collection Clamp @19:56:35.86 Elbow.reconfigure TightElbow @19:56:35.92 Elbow.seek CC @19:56:36.05 Elbow.reconfigure LooseElbow @19:56:36.16 Forearm.seek CC @19:56:37.12 Hand.close @19:56:37.85 CC.seek :open @19:56:42.74 Forearm.seek :retract @19:56:43.73 Elbow.reconfigure TightElbow @19:56:43.80 Elbow.seek :garage @19:56:46.93 Elbow.reconfigure LooseElbow @19:56:47.04 Forearm.reconfigure TightForearm @19:56:47.10 Forearm.seek :garage @19:56:48.27 Hand.open @19:56:49.15 Forearm.reconfigure LooseForearm @19:56:49.21 Forearm.seek :retract @19:56:50.26 Starting initialPurge @19:56:50.27 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:56:50.37 Hand.open @19:56:50.65 Elbow.reconfigure TightElbow @19:56:50.71 Elbow.seek FlushPuck::Garage @19:56:50.84 Elbow.reconfigure LooseElbow @19:56:50.95 Forearm.seek :garage @19:56:51.99 Hand.close @19:56:52.83 Forearm.seek :retract @19:56:53.85 Elbow.reconfigure TightElbow @19:56:53.91 Elbow.seek CC @19:56:56.80 Elbow.reconfigure LooseElbow @19:56:56.91 Forearm.reconfigure TightForearm @19:56:56.96 Forearm.seek CC @19:56:58.23 CC.seek :closed @19:57:02.48 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.37 @19:57:02.57 Hand.open @19:57:03.40 Forearm.reconfigure LooseForearm @19:57:03.46 Forearm.seek :retract @19:57:04.48 Flushing the Collection Valve::Manifold @19:57:04.61 CSV.dial CSR @19:57:05.17 Pulling 5ml of CSR.flush CSR.select :flush @19:57:05.31 CS.reconfigure CSconfig @19:57:05.38 CS.seek 5ml @19:57:19.18 delay 5 seconds @19:57:24.20 CSR.select :air @19:57:24.38 CS.reconfigure CSfast @19:57:24.44 CS.seek 7.5ml @19:57:31.74 CSV.dial :waste @19:57:33.26 CS.reconfigure CSconfig @19:57:33.32 CS.seek 0ml @19:57:53.43 Purging external Intake @19:57:53.56 CSV.dial :flush @19:57:54.09 CS.reconfigure CSfast @19:57:54.15 CS.seek 10ml @19:58:20.62 delay 5 seconds @19:58:25.64 CSV.dial CBV @19:58:25.68
CBV.dial CSV @19:58:26.63 CTV.dial :intake @19:58:27.51 Intake.select :open @19:58:27.64 CS.seek 0ml @19:58:55.15 Intake.select :close @19:58:55.26 CTV.dial :air @19:58:56.12 Flushing upper manifold @19:58:56.77 CSV.dial :flush @19:58:57.64 CS.seek 5ml @19:59:11.40 delay 5 seconds @19:59:16.42 CSV.dial :air @19:59:17.01 CS.seek 7ml @19:59:23.12 CSV.dial CBV @19:59:23.15
CBV.dial CSV @19:59:24.37 CTV.dial :air @19:59:24.39 CS.seek 0ml @19:59:43.15 delay 7 seconds @19:59:50.18 CS.seek 3ml @19:59:58.83 CSV.dial :waste @19:59:59.38 CS.seek 0ml @20:00:08.01 CSV.dial :flush @20:00:08.53 CS.seek 3ml @20:00:17.15 delay 5 seconds @20:00:22.17 CSV.dial :air @20:00:22.74 CS.seek 5ml @20:00:28.84 CSV.dial :air @20:00:28.86 CS.seek 0ml @20:00:42.40 delay 7 seconds @20:00:49.43 CS.seek 3ml @20:00:58.02 CSV.dial :waste @20:00:58.89 CS.seek 0ml @20:01:07.52 Garaging FlushPuck in Collection Clamp @20:01:07.61 Elbow.reconfigure TightElbow @20:01:07.67 Elbow.seek CC @20:01:07.80 Elbow.reconfigure LooseElbow @20:01:07.91 Forearm.seek CC @20:01:08.90 Hand.close @20:01:09.73 CC.seek :open @20:01:14.07 Forearm.seek :retract @20:01:15.04 Elbow.reconfigure TightElbow @20:01:15.10 Elbow.seek :garage @20:01:18.25 Elbow.reconfigure LooseElbow @20:01:18.35 Forearm.reconfigure TightForearm @20:01:18.41 Forearm.seek :garage @20:01:19.58 Hand.open @20:01:20.40 Forearm.reconfigure LooseForearm @20:01:20.46 Forearm.seek :retract @20:01:21.48 Completed [mbaPhase] begun 16:32:17 @20:01:21.50 Extending 18tun_postDeploy3 until completion of MFBPCR @20:47:41.52 PCR.off @20:47:43.57 PCR for Tt1 completed -- closed /var/log/esp/Tt118nov2419h1200ml.pcr @20:47:43.65 Cleaning PCR @20:47:43.66 AFV.select :carrierSolution @20:47:43.79 delay 2 seconds @20:47:45.81 AS.reconfigure ASconfig @20:47:45.88 AS.seek 0.5ml @20:47:56.18 delay 1 second @20:47:57.20 AFV.select :holdingCoil @20:47:57.30 ASV.dial :waste1 @20:47:57.72 ATV.dial :air3 @20:47:59.03 AS.reconfigure ASslow1 @20:47:59.09 AS.seek 0.51ml @20:48:01.39 delay 2 seconds @20:48:03.41 ATV.dial :bleach @20:48:04.04 AS.seek 0.81ml @20:49:04.65 delay 3 seconds @20:49:07.67 ATV.dial :air3 @20:49:08.23 delay 3 seconds @20:49:11.31 AS.seek 0.835ml @20:49:16.62 delay 3 seconds @20:49:19.64 ATV.dial :bleach @20:49:20.26 AS.seek 0.82ml @20:49:23.51 delay 3 seconds @20:49:26.53 ARV.dial :waste @20:49:27.15 delay 3 seconds @20:49:30.17 ATV.dial :ARV @20:49:31.07 AS.reconfigure ASconfig @20:49:31.13 AS.seek 0.47ml @20:49:51.51 AFV.select :carrierSolution @20:49:51.63 delay 2 seconds @20:49:53.65 AS.seek 1ml @20:50:24.43 delay 1 second @20:50:25.45 AFV.select :holdingCoil @20:50:25.56 delay 1 second @20:50:26.58 AS.reconfigure ASfast @20:50:26.65 AS.seek 0ml @20:50:54.04 AFV.select :carrierSolution @20:50:54.16 delay 2 seconds @20:50:56.18 AS.reconfigure ASconfig @20:50:56.24 AS.seek 1ml @20:51:54.01 delay 1 second @20:51:55.03 AFV.select :holdingCoil @20:51:55.14 delay 1 second @20:51:56.16 AS.reconfigure ASfast @20:51:56.23 AS.seek 0ml @20:52:23.57 AFV.select :carrierSolution @20:52:23.69 delay 2 seconds @20:52:25.71 AS.reconfigure ASconfig @20:52:25.78 AS.seek 1ml @20:53:23.56 delay 1 second @20:53:24.58 AFV.select :holdingCoil @20:53:24.69 delay 1 second @20:53:25.71 AS.reconfigure ASfast @20:53:25.77 AS.seek 0ml @20:53:53.12 ARV.dial between carrier and air1 @20:53:53.64 ATV.dial :air3 @20:53:54.93 AS.reconfigure ASslow1 @20:53:54.99 AS.seek 0.01ml @20:53:57.36 delay 2 seconds @20:53:59.38 ATV.dial :bleach @20:54:00.01 AS.seek 0.81ml @20:56:41.20 delay 3 seconds @20:56:44.22 ATV.dial :air3 @20:56:44.78 delay 3 seconds @20:56:47.86 AS.seek 0.835ml @20:56:53.16 delay 3 seconds @20:56:56.18 ATV.dial :bleach @20:56:56.81 AS.seek 0.82ml @20:57:00.08 delay 3 seconds @20:57:03.10 ATV.dial PCR @20:57:03.50 AS.reconfigure ASconfig @20:57:03.56 AS.seek :empty @20:57:51.01 PCR.program :bleachProfile @20:57:51.07 PCR program should complete in < 2:45 @20:57:51.08 PCR.run 10:00 @21:00:49.88 PCR.off @21:00:49.92 AFV.select :carrierSolution @21:00:50.04 delay 2 seconds @21:00:52.06 AS.seek 1ml @21:01:49.90 delay 1 second @21:01:50.92 AFV.select :holdingCoil @21:01:51.48 delay 1 second @21:01:52.57 AS.reconfigure ASfast @21:01:52.64 AS.seek 0ml @21:02:19.98 delay 3 seconds @21:02:23.00 AFV.select :carrierSolution @21:02:23.11 delay 2 seconds @21:02:25.13 AS.reconfigure ASconfig @21:02:25.20 AS.seek 1ml @21:03:23.00 delay 1 second @21:03:24.02 AFV.select :holdingCoil @21:03:24.13 delay 1 second @21:03:25.15 AS.reconfigure ASfast @21:03:25.22 AS.seek 0ml @21:03:52.56 delay 3 seconds @21:03:55.58 AFV.select :carrierSolution @21:03:55.69 delay 2 seconds @21:03:57.71 AS.reconfigure ASconfig @21:03:57.77 AS.seek 1ml @21:04:55.53 delay 1 second @21:04:56.55 AFV.select :holdingCoil @21:04:56.66 delay 1 second @21:04:57.68 AS.reconfigure ASfast @21:04:57.75 AS.seek 0ml @21:05:25.11 delay 3 seconds @21:05:28.13 AFV.select :carrierSolution @21:05:28.24 delay 2 seconds @21:05:30.27 AS.reconfigure ASconfig @21:05:30.32 AS.seek 1ml @21:06:28.08 delay 1 second @21:06:29.10 AFV.select :holdingCoil @21:06:29.21 delay 1 second @21:06:30.23 AS.reconfigure ASfast @21:06:30.30 AS.seek 0ml @21:06:57.65 delay 3 seconds @21:07:00.67 AFV.select :carrierSolution @21:07:00.78 delay 2 seconds @21:07:02.81 AS.reconfigure ASconfig @21:07:02.88 AS.seek 1ml @21:08:00.65 delay 1 second @21:08:01.67 AFV.select :holdingCoil @21:08:01.78 delay 1 second @21:08:02.80 AS.reconfigure ASnorm @21:08:02.86 AS.seek 0ml @21:08:53.60 Setting up PCR for Rp -- writing /var/log/esp/Rp18nov2421h1200ml.pcr @21:08:53.62 Building PCR Slug from ASV with MM2 mix, PP3 primer @21:08:53.65 Priming with carrier @21:08:53.67 ATV.dial ARV @21:08:54.30 ARV.dial :carrier @21:08:54.65 AS.seek 0.5ml @21:09:20.28 AFV.select :carrierSolution @21:09:20.41 delay 2 seconds @21:09:22.43 AS.reconfigure ASconfig @21:09:22.50 AS.seek 1ml @21:09:51.51 delay 1 second @21:09:52.53 AFV.select :holdingCoil @21:09:52.64 delay 1 second @21:09:53.65 ATV.dial :waste @21:09:55.34 AS.reconfigure ASnorm @21:09:55.40 AS.seek :empty @21:10:46.14 AFV.select :carrierSolution @21:10:46.27 delay 2 seconds @21:10:48.29 AS.reconfigure ASconfig @21:10:48.36 AS.seek 0.5ml @21:11:17.48 delay 1 second @21:11:18.50 AFV.select :holdingCoil @21:11:18.61 Building Slug @21:11:18.62 ATV.dial :ARV @21:11:20.37 delay 2 seconds @21:11:22.39 ARV.dial :air1 @21:11:22.81 delay 2 seconds @21:11:24.88 AS.reconfigure ASslow2 @21:11:24.95 AS.seek 0.506ml @21:11:31.19 delay 2 seconds @21:11:33.21 ARV.dial :MM2 @21:11:34.73 delay 2 seconds @21:11:36.76 Adding MM2 @21:11:36.84 AS.seek 0.524ml @21:11:55.17 delay 25 seconds @21:12:20.19 ARV.dial :PP3 @21:12:20.59 Adding PP3 @21:12:20.61 delay 2 seconds @21:12:22.69 AS.seek 0.53ml @21:12:29.25 delay 10 seconds @21:12:39.28 ARV.dial :air2 @21:12:39.70 adding ARV air @21:12:39.72 delay 2 seconds @21:12:41.80 AS.seek 0.536ml @21:12:48.17 delay 5 seconds @21:12:53.19 ARV.dial :carrier @21:12:54.50 delay 7 seconds @21:13:01.59 AS.seek 0.594ml @21:14:00.05 delay 5 seconds @21:14:05.07 ATV.dial :ASV @21:14:05.48 delay 2 seconds @21:14:07.56 AS.seek 0.6ml @21:14:13.84 delay 4 seconds @21:14:17.86 ATV.dial ARV @21:14:18.42 delay 2 seconds @21:14:20.44 ARV.dial :carrier @21:14:20.46 delay 2 seconds @21:14:22.54 AS.seek 0.65ml @21:15:13.06 delay 4 seconds @21:15:17.08 ATV.dial PCR @21:15:17.70 ASV.dial :waste1 @21:15:17.71 ARV.dial :air1 @21:15:18.66 Positioning Slug by 0.324ml @21:15:18.75 AS.seek 0.326ml @21:20:44.80 delay 5 seconds @21:20:49.82 ATV.dial ARV @21:20:50.61 ASV.dial :PCRlock @21:20:51.01 delay 5 seconds @21:20:56.04 Running Rp Profile60C300 thermocycling @21:20:56.05 PCR.program Profile60C300 @21:20:56.19 Profile60C300 should complete in < 1:45:21 @21:20:56.21 PCR.run 2:16:41.25 @22:42:43.32 PCR.off @22:42:44.84 PCR for Rp completed -- closed /var/log/esp/Rp18nov2421h1200ml.pcr @22:42:45.43 Cleaning PCR @22:42:45.44 AFV.select :carrierSolution @22:42:45.57 delay 2 seconds @22:42:47.59 AS.reconfigure ASconfig @22:42:47.65 AS.seek 0.5ml @22:42:57.96 delay 1 second @22:42:58.98 AFV.select :holdingCoil @22:42:59.08 ASV.dial :waste1 @22:42:59.49 ATV.dial :air3 @22:43:00.78 AS.reconfigure ASslow1 @22:43:00.84 AS.seek 0.51ml @22:43:03.14 delay 2 seconds @22:43:05.16 ATV.dial :bleach @22:43:05.78 AS.seek 0.81ml @22:44:06.38 delay 3 seconds @22:44:09.40 ATV.dial :air3 @22:44:09.96 delay 3 seconds @22:44:13.03 AS.seek 0.835ml @22:44:18.35 delay 3 seconds @22:44:21.37 ATV.dial :bleach @22:44:21.99 AS.seek 0.82ml @22:44:25.29 delay 3 seconds @22:44:28.31 ARV.dial :waste @22:44:28.96 delay 3 seconds @22:44:31.98 ATV.dial :ARV @22:44:32.88 AS.reconfigure ASconfig @22:44:32.94 AS.seek 0.47ml @22:44:53.35 AFV.select :carrierSolution @22:44:53.47 delay 2 seconds @22:44:55.49 AS.seek 1ml @22:45:26.27 delay 1 second @22:45:27.29 AFV.select :holdingCoil @22:45:27.40 delay 1 second @22:45:28.42 AS.reconfigure ASfast @22:45:28.49 AS.seek 0ml @22:45:55.87 AFV.select :carrierSolution @22:45:55.99 delay 2 seconds @22:45:58.01 AS.reconfigure ASconfig @22:45:58.07 AS.seek 1ml @22:46:55.85 delay 1 second @22:46:56.87 AFV.select :holdingCoil @22:46:56.98 delay 1 second @22:46:58.00 AS.reconfigure ASfast @22:46:58.07 AS.seek 0ml @22:47:25.43 AFV.select :carrierSolution @22:47:25.55 delay 2 seconds @22:47:27.57 AS.reconfigure ASconfig @22:47:27.63 AS.seek 1ml @22:48:25.43 delay 1 second @22:48:26.45 AFV.select :holdingCoil @22:48:26.56 delay 1 second @22:48:27.58 AS.reconfigure ASfast @22:48:27.65 AS.seek 0ml @22:48:55.01 ARV.dial between carrier and air1 @22:48:55.54 ATV.dial :air3 @22:48:56.85 AS.reconfigure ASslow1 @22:48:56.91 AS.seek 0.01ml @22:48:59.24 delay 2 seconds @22:49:01.26 ATV.dial :bleach @22:49:01.88 AS.seek 0.81ml @22:51:43.05 delay 3 seconds @22:51:46.07 ATV.dial :air3 @22:51:46.63 delay 3 seconds @22:51:49.70 AS.seek 0.835ml @22:51:55.02 delay 3 seconds @22:51:58.04 ATV.dial :bleach @22:51:58.66 AS.seek 0.82ml @22:52:01.93 delay 3 seconds @22:52:04.95 ATV.dial PCR @22:52:05.35 AS.reconfigure ASconfig @22:52:05.41 AS.seek :empty @22:52:52.90 PCR.program :bleachProfile @22:52:52.96 PCR program should complete in < 2:45 @22:52:52.97 PCR.run 10:00 @22:55:51.78 PCR.off @22:55:51.82 AFV.select :carrierSolution @22:55:51.94 delay 2 seconds @22:55:53.96 AS.seek 1ml @22:56:51.80 delay 1 second @22:56:52.82 AFV.select :holdingCoil @22:56:52.93 delay 1 second @22:56:53.95 AS.reconfigure ASfast @22:56:54.01 AS.seek 0ml @22:57:21.35 delay 3 seconds @22:57:24.37 AFV.select :carrierSolution @22:57:24.48 delay 2 seconds @22:57:26.50 AS.reconfigure ASconfig @22:57:26.56 AS.seek 1ml @22:58:24.30 delay 1 second @22:58:25.32 AFV.select :holdingCoil @22:58:25.88 delay 1 second @22:58:26.92 AS.reconfigure ASfast @22:58:26.99 AS.seek 0ml @22:58:54.43 delay 3 seconds @22:58:57.45 AFV.select :carrierSolution @22:58:57.56 delay 2 seconds @22:58:59.58 AS.reconfigure ASconfig @22:58:59.65 AS.seek 1ml @22:59:57.41 delay 1 second @22:59:58.43 AFV.select :holdingCoil @22:59:58.54 delay 1 second @22:59:59.56 AS.reconfigure ASfast @22:59:59.63 AS.seek 0ml @23:00:26.99 delay 3 seconds @23:00:30.01 AFV.select :carrierSolution @23:00:30.12 delay 2 seconds @23:00:32.14 AS.reconfigure ASconfig @23:00:32.20 AS.seek 1ml @23:01:29.98 delay 1 second @23:01:31.00 AFV.select :holdingCoil @23:01:31.11 delay 1 second @23:01:32.13 AS.reconfigure ASfast @23:01:32.20 AS.seek 0ml @23:01:59.60 delay 3 seconds @23:02:02.62 AFV.select :carrierSolution @23:02:02.73 delay 2 seconds @23:02:04.75 AS.reconfigure ASconfig @23:02:04.81 AS.seek 1ml @23:03:02.62 delay 1 second @23:03:03.64 AFV.select :holdingCoil @23:03:03.75 delay 1 second @23:03:04.77 AS.reconfigure ASnorm @23:03:04.83 AS.seek 0ml @23:03:55.54 Setting up PCR for Bb -- writing /var/log/esp/Bb18nov2423h1200ml.pcr @23:03:55.56 Building PCR Slug from ASV with MM3 mix, PP5 primer @23:03:55.58 Priming with carrier @23:03:55.60 ATV.dial ARV @23:03:56.34 ARV.dial :carrier @23:03:56.69 AS.seek 0.5ml @23:04:22.23 AFV.select :carrierSolution @23:04:22.36 delay 2 seconds @23:04:24.38 AS.reconfigure ASconfig @23:04:24.45 AS.seek 1ml @23:04:53.46 delay 1 second @23:04:54.48 AFV.select :holdingCoil @23:04:54.59 delay 1 second @23:04:55.60 ATV.dial :waste @23:04:57.28 AS.reconfigure ASnorm @23:04:57.34 AS.seek :empty @23:05:48.09 AFV.select :carrierSolution @23:05:48.22 delay 2 seconds @23:05:50.24 AS.reconfigure ASconfig @23:05:50.31 AS.seek 0.5ml @23:06:19.38 delay 1 second @23:06:20.40 AFV.select :holdingCoil @23:06:20.51 Building Slug @23:06:20.52 ATV.dial :ARV @23:06:22.27 delay 2 seconds @23:06:24.29 ARV.dial :air1 @23:06:24.71 delay 2 seconds @23:06:26.78 AS.reconfigure ASslow2 @23:06:26.85 AS.seek 0.506ml @23:06:33.07 delay 2 seconds @23:06:35.09 ARV.dial :MM3 @23:06:36.16 delay 2 seconds @23:06:38.19 Adding MM3 @23:06:38.28 AS.seek 0.524ml @23:06:56.48 delay 25 seconds @23:07:21.50 ARV.dial :PP5 @23:07:21.90 Adding PP5 @23:07:21.92 delay 2 seconds @23:07:24.00 AS.seek 0.53ml @23:07:30.27 delay 10 seconds @23:07:40.30 ARV.dial :air3 @23:07:40.71 adding ARV air @23:07:40.73 delay 2 seconds @23:07:42.80 AS.seek 0.536ml @23:07:49.23 delay 5 seconds @23:07:54.25 ARV.dial :carrier @23:07:55.56 delay 7 seconds @23:08:02.65 AS.seek 0.594ml @23:09:01.23 delay 5 seconds @23:09:06.25 ATV.dial :ASV @23:09:06.66 delay 2 seconds @23:09:08.74 AS.seek 0.6ml @23:09:15.12 delay 4 seconds @23:09:19.14 ATV.dial ARV @23:09:19.70 delay 2 seconds @23:09:21.72 ARV.dial :carrier @23:09:21.74 delay 2 seconds @23:09:23.82 AS.seek 0.65ml @23:10:14.29 delay 4 seconds @23:10:18.31 ATV.dial PCR @23:10:18.93 ASV.dial :waste1 @23:10:18.94 ARV.dial :air1 @23:10:19.38 Positioning Slug by 0.324ml @23:10:19.48 AS.seek 0.326ml @23:15:45.60 delay 5 seconds @23:15:50.62 ATV.dial ARV @23:15:51.40 ASV.dial :PCRlock @23:15:52.31 delay 5 seconds @23:15:57.35 Running Bb Profile60C300 thermocycling @23:15:57.36 PCR.program Profile60C300 @23:15:57.50 Profile60C300 should complete in < 1:45:21 @23:15:57.52 PCR.run 2:16:41.25 @00:37:38.64CET25-Nov-18 PCR.off @00:37:40.17 PCR for Bb completed -- closed /var/log/esp/Bb18nov2423h1200ml.pcr @00:37:40.25 Cleaning PCR @00:37:40.27 AFV.select :carrierSolution @00:37:40.40 delay 2 seconds @00:37:42.42 AS.reconfigure ASconfig @00:37:42.48 AS.seek 0.5ml @00:37:52.78 delay 1 second @00:37:53.80 AFV.select :holdingCoil @00:37:53.90 ASV.dial :waste1 @00:37:54.30 ATV.dial :air3 @00:37:55.59 AS.reconfigure ASslow1 @00:37:55.65 AS.seek 0.51ml @00:37:58.03 delay 2 seconds @00:38:00.05 ATV.dial :bleach @00:38:01.18 AS.seek 0.81ml @00:39:01.78 delay 3 seconds @00:39:04.80 ATV.dial :air3 @00:39:05.36 delay 3 seconds @00:39:08.44 AS.seek 0.835ml @00:39:13.74 delay 3 seconds @00:39:16.76 ATV.dial :bleach @00:39:17.39 AS.seek 0.82ml @00:39:20.72 delay 3 seconds @00:39:23.74 ARV.dial :waste @00:39:24.36 delay 3 seconds @00:39:27.38 ATV.dial :ARV @00:39:28.28 AS.reconfigure ASconfig @00:39:28.34 AS.seek 0.47ml @00:39:48.77 AFV.select :carrierSolution @00:39:48.89 delay 2 seconds @00:39:50.91 AS.seek 1ml @00:40:21.70 delay 1 second @00:40:22.72 AFV.select :holdingCoil @00:40:22.83 delay 1 second @00:40:23.85 AS.reconfigure ASfast @00:40:23.92 AS.seek 0ml @00:40:51.28 AFV.select :carrierSolution @00:40:51.40 delay 2 seconds @00:40:53.42 AS.reconfigure ASconfig @00:40:53.48 AS.seek 1ml @00:41:51.22 delay 1 second @00:41:52.24 AFV.select :holdingCoil @00:41:52.35 delay 1 second @00:41:53.37 AS.reconfigure ASfast @00:41:53.44 AS.seek 0ml @00:42:20.80 AFV.select :carrierSolution @00:42:20.92 delay 2 seconds @00:42:22.94 AS.reconfigure ASconfig @00:42:23.00 AS.seek 1ml @00:43:20.80 delay 1 second @00:43:21.82 AFV.select :holdingCoil @00:43:21.93 delay 1 second @00:43:22.95 AS.reconfigure ASfast @00:43:23.02 AS.seek 0ml @00:43:50.36 ARV.dial between carrier and air1 @00:43:50.88 ATV.dial :air3 @00:43:52.19 AS.reconfigure ASslow1 @00:43:52.25 AS.seek 0.01ml @00:43:54.61 delay 2 seconds @00:43:56.63 ATV.dial :bleach @00:43:57.25 AS.seek 0.81ml @00:46:38.42 delay 3 seconds @00:46:41.44 ATV.dial :air3 @00:46:42.00 delay 3 seconds @00:46:45.07 AS.seek 0.835ml @00:46:50.39 delay 3 seconds @00:46:53.41 ATV.dial :bleach @00:46:54.03 AS.seek 0.82ml @00:46:57.34 delay 3 seconds @00:47:00.36 ATV.dial PCR @00:47:00.77 AS.reconfigure ASconfig @00:47:00.83 AS.seek :empty @00:47:48.31 PCR.program :bleachProfile @00:47:48.37 PCR program should complete in < 2:45 @00:47:48.38 PCR.run 10:00 @00:50:47.19 PCR.off @00:50:47.23 AFV.select :carrierSolution @00:50:47.35 delay 2 seconds @00:50:49.37 AS.seek 1ml @00:51:47.22 delay 1 second @00:51:48.24 AFV.select :holdingCoil @00:51:48.35 delay 1 second @00:51:49.37 AS.reconfigure ASfast @00:51:49.44 AS.seek 0ml @00:52:16.80 delay 3 seconds @00:52:19.82 AFV.select :carrierSolution @00:52:19.93 delay 2 seconds @00:52:21.95 AS.reconfigure ASconfig @00:52:22.01 AS.seek 1ml @00:53:19.75 delay 1 second @00:53:20.77 AFV.select :holdingCoil @00:53:20.88 delay 1 second @00:53:21.90 AS.reconfigure ASfast @00:53:21.96 AS.seek 0ml @00:53:49.31 delay 3 seconds @00:53:52.33 AFV.select :carrierSolution @00:53:52.44 delay 2 seconds @00:53:54.46 AS.reconfigure ASconfig @00:53:54.53 AS.seek 1ml @00:54:52.31 delay 1 second @00:54:53.33 AFV.select :holdingCoil @00:54:53.44 delay 1 second @00:54:54.46 AS.reconfigure ASfast @00:54:54.52 AS.seek 0ml @00:55:21.88 delay 3 seconds @00:55:24.90 AFV.select :carrierSolution @00:55:25.01 delay 2 seconds @00:55:27.03 AS.reconfigure ASconfig @00:55:27.09 AS.seek 1ml @00:56:24.96 delay 1 second @00:56:25.97 AFV.select :holdingCoil @00:56:26.08 delay 1 second @00:56:27.10 AS.reconfigure ASfast @00:56:27.17 AS.seek 0ml @00:56:54.52 delay 3 seconds @00:56:57.54 AFV.select :carrierSolution @00:56:57.65 delay 2 seconds @00:56:59.67 AS.reconfigure ASconfig @00:56:59.74 AS.seek 1ml @00:57:57.52 delay 1 second @00:57:58.54 AFV.select :holdingCoil @00:57:58.65 delay 1 second @00:57:59.67 AS.reconfigure ASnorm @00:57:59.74 AS.seek 0ml @00:58:50.48 Setting up PCR for Ss -- writing /var/log/esp/Ss18nov2500h1200ml.pcr @00:58:50.50 Building PCR Slug from ASV with MM1 mix, PP2 primer @00:58:50.51 Priming with carrier @00:58:50.53 ATV.dial ARV @00:58:51.17 ARV.dial :carrier @00:58:51.53 AS.seek 0.5ml @00:59:17.10 AFV.select :carrierSolution @00:59:17.23 delay 2 seconds @00:59:19.25 AS.reconfigure ASconfig @00:59:19.32 AS.seek 1ml @00:59:48.35 delay 1 second @00:59:49.37 AFV.select :holdingCoil @00:59:49.48 delay 1 second @00:59:50.49 ATV.dial :waste @00:59:52.18 AS.reconfigure ASnorm @00:59:52.24 AS.seek :empty @01:00:43.00 AFV.select :carrierSolution @01:00:43.13 delay 2 seconds @01:00:45.15 AS.reconfigure ASconfig @01:00:45.22 AS.seek 0.5ml @01:01:14.28 delay 1 second @01:01:15.30 AFV.select :holdingCoil @01:01:15.41 Building Slug @01:01:15.42 ATV.dial :ARV @01:01:17.08 delay 2 seconds @01:01:19.10 ARV.dial :air1 @01:01:19.51 delay 2 seconds @01:01:21.58 AS.reconfigure ASslow2 @01:01:21.65 AS.seek 0.506ml @01:01:28.13 delay 2 seconds @01:01:30.15 ARV.dial :MM1 @01:01:30.75 delay 2 seconds @01:01:32.78 Adding MM1 @01:01:32.86 AS.seek 0.524ml @01:01:51.28 delay 25 seconds @01:02:16.30 ARV.dial :PP2 @01:02:16.71 Adding PP2 @01:02:16.72 delay 2 seconds @01:02:18.81 AS.seek 0.53ml @01:02:25.24 delay 10 seconds @01:02:35.27 ARV.dial :air2 @01:02:35.67 adding ARV air @01:02:35.69 delay 2 seconds @01:02:37.78 AS.seek 0.536ml @01:02:44.02 delay 5 seconds @01:02:49.04 ARV.dial :carrier @01:02:50.35 delay 7 seconds @01:02:57.44 AS.seek 0.594ml @01:03:55.97 delay 5 seconds @01:04:00.99 ATV.dial :ASV @01:04:01.39 delay 2 seconds @01:04:03.46 AS.seek 0.6ml @01:04:09.72 delay 4 seconds @01:04:13.74 ATV.dial ARV @01:04:14.30 delay 2 seconds @01:04:16.32 ARV.dial :carrier @01:04:16.34 delay 2 seconds @01:04:18.42 AS.seek 0.65ml @01:05:09.00 delay 4 seconds @01:05:13.02 ATV.dial PCR @01:05:13.64 ASV.dial :waste1 @01:05:13.65 ARV.dial :air1 @01:05:14.08 Positioning Slug by 0.324ml @01:05:14.17 AS.seek 0.326ml @01:10:40.24 delay 5 seconds @01:10:45.26 ATV.dial ARV @01:10:46.03 ASV.dial :PCRlock @01:10:46.43 delay 5 seconds @01:10:51.46 Running Ss Profile60C350 thermocycling @01:10:51.47 PCR.program Profile60C350 @01:10:51.63 Profile60C350 should complete in < 1:48:05 @01:10:51.64 PCR.run 2:20:06.25 @02:33:00.68 PCR.off @02:33:02.69 PCR for Ss completed -- closed /var/log/esp/Ss18nov2500h1200ml.pcr @02:33:02.76 Cleaning PCR @02:33:02.77 AFV.select :carrierSolution @02:33:02.90 delay 2 seconds @02:33:04.92 AS.reconfigure ASconfig @02:33:04.98 AS.seek 0.5ml @02:33:15.31 delay 1 second @02:33:16.33 AFV.select :holdingCoil @02:33:16.43 ASV.dial :waste1 @02:33:16.90 ATV.dial :air3 @02:33:18.20 AS.reconfigure ASslow1 @02:33:18.26 AS.seek 0.51ml @02:33:20.58 delay 2 seconds @02:33:22.59 ATV.dial :bleach @02:33:23.71 AS.seek 0.81ml @02:34:24.31 delay 3 seconds @02:34:27.33 ATV.dial :air3 @02:34:27.90 delay 3 seconds @02:34:30.98 AS.seek 0.835ml @02:34:36.29 delay 3 seconds @02:34:39.31 ATV.dial :bleach @02:34:39.93 AS.seek 0.82ml @02:34:43.28 delay 3 seconds @02:34:46.30 ARV.dial :waste @02:34:46.95 delay 3 seconds @02:34:49.97 ATV.dial :ARV @02:34:50.87 AS.reconfigure ASconfig @02:34:50.93 AS.seek 0.47ml @02:35:11.34 AFV.select :carrierSolution @02:35:11.46 delay 2 seconds @02:35:13.48 AS.seek 1ml @02:35:44.28 delay 1 second @02:35:45.30 AFV.select :holdingCoil @02:35:45.41 delay 1 second @02:35:46.43 AS.reconfigure ASfast @02:35:46.50 AS.seek 0ml @02:36:13.86 AFV.select :carrierSolution @02:36:13.98 delay 2 seconds @02:36:16.00 AS.reconfigure ASconfig @02:36:16.06 AS.seek 1ml @02:37:13.84 delay 1 second @02:37:14.86 AFV.select :holdingCoil @02:37:14.97 delay 1 second @02:37:15.99 AS.reconfigure ASfast @02:37:16.05 AS.seek 0ml @02:37:43.40 AFV.select :carrierSolution @02:37:43.52 delay 2 seconds @02:37:45.54 AS.reconfigure ASconfig @02:37:45.60 AS.seek 1ml @02:38:43.43 delay 1 second @02:38:44.45 AFV.select :holdingCoil @02:38:44.56 delay 1 second @02:38:45.58 AS.reconfigure ASfast @02:38:45.65 AS.seek 0ml @02:39:13.01 ARV.dial between carrier and air1 @02:39:13.53 ATV.dial :air3 @02:39:14.84 AS.reconfigure ASslow1 @02:39:14.90 AS.seek 0.01ml @02:39:17.21 delay 2 seconds @02:39:19.23 ATV.dial :bleach @02:39:19.85 AS.seek 0.81ml @02:42:01.00 delay 3 seconds @02:42:04.02 ATV.dial :air3 @02:42:04.59 delay 3 seconds @02:42:07.66 AS.seek 0.835ml @02:42:12.97 delay 3 seconds @02:42:15.99 ATV.dial :bleach @02:42:16.62 AS.seek 0.82ml @02:42:19.93 delay 3 seconds @02:42:22.95 ATV.dial PCR @02:42:23.36 AS.reconfigure ASconfig @02:42:23.42 AS.seek :empty @02:43:10.89 PCR.program :bleachProfile @02:43:10.94 PCR program should complete in < 2:45 @02:43:10.95 PCR.run 10:00 @02:46:09.76 PCR.off @02:46:09.79 AFV.select :carrierSolution @02:46:09.92 delay 2 seconds @02:46:11.94 AS.seek 1ml @02:47:09.76 delay 1 second @02:47:10.78 AFV.select :holdingCoil @02:47:10.89 delay 1 second @02:47:11.91 AS.reconfigure ASfast @02:47:11.97 AS.seek 0ml @02:47:39.31 delay 3 seconds @02:47:42.33 AFV.select :carrierSolution @02:47:42.44 delay 2 seconds @02:47:44.46 AS.reconfigure ASconfig @02:47:44.53 AS.seek 1ml @02:48:42.32 delay 1 second @02:48:43.34 AFV.select :holdingCoil @02:48:43.45 delay 1 second @02:48:44.47 AS.reconfigure ASfast @02:48:44.53 AS.seek 0ml @02:49:11.90 delay 3 seconds @02:49:14.92 AFV.select :carrierSolution @02:49:15.03 delay 2 seconds @02:49:17.05 AS.reconfigure ASconfig @02:49:17.11 AS.seek 1ml @02:50:14.93 delay 1 second @02:50:15.95 AFV.select :holdingCoil @02:50:16.06 delay 1 second @02:50:17.08 AS.reconfigure ASfast @02:50:17.14 AS.seek 0ml @02:50:44.51 delay 3 seconds @02:50:47.53 AFV.select :carrierSolution @02:50:47.64 delay 2 seconds @02:50:49.66 AS.reconfigure ASconfig @02:50:49.74 AS.seek 1ml @02:51:48.03 delay 1 second @02:51:49.05 AFV.select :holdingCoil @02:51:49.16 delay 1 second @02:51:50.18 AS.reconfigure ASfast @02:51:50.25 AS.seek 0ml @02:52:17.59 delay 3 seconds @02:52:20.61 AFV.select :carrierSolution @02:52:20.72 delay 2 seconds @02:52:22.74 AS.reconfigure ASconfig @02:52:22.80 AS.seek 1ml @02:53:20.54 delay 1 second @02:53:21.56 AFV.select :holdingCoil @02:53:21.67 delay 1 second @02:53:22.69 AS.reconfigure ASnorm @02:53:22.76 AS.seek 0ml @02:54:13.50 Setting up PCR for IPc -- writing /var/log/esp/IPc18nov2502h1200ml.pcr @02:54:13.52 Building PCR Slug from ASV with MM1 mix, PP1 primer @02:54:13.54 Priming with carrier @02:54:13.56 ATV.dial ARV @02:54:14.20 ARV.dial :carrier @02:54:14.57 AS.seek 0.5ml @02:54:40.15 AFV.select :carrierSolution @02:54:40.28 delay 2 seconds @02:54:42.30 AS.reconfigure ASconfig @02:54:42.37 AS.seek 1ml @02:55:11.45 delay 1 second @02:55:12.47 AFV.select :holdingCoil @02:55:12.58 delay 1 second @02:55:13.59 ATV.dial :waste @02:55:15.29 AS.reconfigure ASnorm @02:55:15.35 AS.seek :empty @02:56:06.14 AFV.select :carrierSolution @02:56:06.27 delay 2 seconds @02:56:08.29 AS.reconfigure ASconfig @02:56:08.36 AS.seek 0.5ml @02:56:37.45 delay 1 second @02:56:38.47 AFV.select :holdingCoil @02:56:38.58 Building Slug @02:56:38.59 ATV.dial :ARV @02:56:40.25 delay 2 seconds @02:56:42.27 ARV.dial :air1 @02:56:42.69 delay 2 seconds @02:56:44.76 AS.reconfigure ASslow2 @02:56:44.83 AS.seek 0.506ml @02:56:51.15 delay 2 seconds @02:56:53.17 ARV.dial :MM1 @02:56:53.78 delay 2 seconds @02:56:55.81 Adding MM1 @02:56:55.89 AS.seek 0.524ml @02:57:14.17 delay 25 seconds @02:57:39.19 ARV.dial :PP1 @02:57:39.59 Adding PP1 @02:57:39.61 delay 2 seconds @02:57:41.69 AS.seek 0.53ml @02:57:48.03 delay 10 seconds @02:57:58.06 ARV.dial :air1 @02:57:58.47 adding ARV air @02:57:58.49 delay 2 seconds @02:58:00.57 AS.seek 0.536ml @02:58:07.00 delay 5 seconds @02:58:12.02 ARV.dial :carrier @02:58:12.44 delay 7 seconds @02:58:19.53 AS.seek 0.594ml @02:59:18.26 delay 5 seconds @02:59:23.28 ATV.dial :ASV @02:59:23.69 delay 2 seconds @02:59:25.77 AS.seek 0.6ml @02:59:32.18 delay 4 seconds @02:59:36.20 ATV.dial ARV @02:59:36.76 delay 2 seconds @02:59:38.78 ARV.dial :carrier @02:59:38.80 delay 2 seconds @02:59:40.88 AS.seek 0.65ml @03:00:31.44 delay 4 seconds @03:00:35.46 ATV.dial PCR @03:00:36.08 ASV.dial :waste1 @03:00:36.09 ARV.dial :air1 @03:00:36.52 Positioning Slug by 0.324ml @03:00:36.62 AS.seek 0.326ml @03:06:02.58 delay 5 seconds @03:06:07.60 ATV.dial ARV @03:06:08.42 ASV.dial :PCRlock @03:06:08.81 delay 5 seconds @03:06:13.84 Running IPc ProfileIPc thermocycling @03:06:13.85 PCR.program ProfileIPc @03:06:14.01 ProfileIPc should complete in < 1:50:49 @03:06:14.02 PCR.run 2:23:31.25 @04:16:41.49 Session Ended @04:29:53.61 PCR.off @04:29:56.64 PCR for IPc completed -- closed /var/log/esp/IPc18nov2502h1200ml.pcr @04:29:56.65 Full MFB Decontamination @04:29:56.66 Decon extraction column and decon AIV @04:29:56.68 SPE.hold 2:46:40,55C @04:29:56.72 ASV.dial :SPEdelivery @04:29:57.37 delay 20 seconds @04:30:17.40 AFV.select :carrierSolution @04:30:17.51 delay 2 seconds @04:30:19.53 AS.reconfigure ASconfig @04:30:19.58 AS.seek 0.5ml @04:30:29.90 delay 1 second @04:30:30.92 AFV.select :holdingCoil @04:30:31.02 ATV.dial :air1 @04:30:32.96 AS.reconfigure ASslow1 @04:30:33.00 AS.seek 0.52ml @04:30:37.32 ATV.dial :ASV @04:30:39.12 ASV.dial :SPErecovery @04:30:39.78 AS.seek 0.62ml @04:31:00.16 ATV.dial :waste @04:31:02.20 AS.reconfigure ASnorm @04:31:02.24 AS.seek :empty @04:31:33.87 AFV.select :holdingCoil @04:31:33.88 ATV.dial :waste @04:31:33.90 AS.reconfigure ASconfig @04:31:33.94 AS.seek 0ml @04:31:34.21 AFV.select :carrierSolution @04:31:34.32 delay 2 seconds @04:31:36.34 AS.seek 0.5ml @04:32:05.35 delay 1 second @04:32:06.37 AFV.select :holdingCoil @04:32:06.47 ATV.dial :air3 @04:32:07.19 AS.reconfigure ASslow1 @04:32:07.23 AS.seek 0.52ml @04:32:11.54 ATV.dial :air3 @04:32:11.61 AS.seek 0.53ml @04:32:13.87 delay 2 seconds @04:32:15.89 ATV.dial :bleach @04:32:16.36 AS.seek 0.71ml @04:32:52.80 delay 3 seconds @04:32:55.82 ATV.dial :air3 @04:32:56.40 delay 3 seconds @04:32:59.47 AS.seek 0.735ml @04:33:04.77 delay 3 seconds @04:33:07.79 ATV.dial :bleach @04:33:08.43 AS.seek 0.72ml @04:33:11.69 delay 3 seconds @04:33:14.71 ATV.dial ASV @04:33:15.80 ASV.dial :SPErecovery @04:33:15.87 AS.seek 0.365ml @04:34:27.52 ATV.dial :waste @04:34:29.29 AS.reconfigure ASnorm @04:34:29.34 AS.seek :empty @04:34:48.12 ATV.dial :air3 @04:34:48.80 AS.reconfigure ASslow1 @04:34:48.84 AS.seek 0.009ml @04:34:51.21 delay 2 seconds @04:34:53.23 ATV.dial :bleach @04:34:53.69 AS.seek 0.579ml @04:36:48.58 delay 3 seconds @04:36:51.60 ATV.dial :air3 @04:36:52.16 delay 3 seconds @04:36:55.23 AS.seek 0.604ml @04:37:00.51 delay 3 seconds @04:37:03.53 ATV.dial :bleach @04:37:04.14 AS.seek 0.589ml @04:37:07.41 delay 3 seconds @04:37:10.43 ATV.dial :ASV @04:37:11.48 ASV.dial :wasteR @04:37:11.95 AS.seek 0ml @04:39:10.71 delay 1:00 @04:40:10.74 ATV.dial ASV @04:40:10.75 AFV.select :carrierSolution @04:40:10.86 delay 2 seconds @04:40:12.88 AS.reconfigure ASconfig @04:40:12.93 AS.seek 1ml @04:41:10.69 delay 1 second @04:41:11.71 AFV.select :holdingCoil @04:41:11.82 delay 1 second @04:41:12.83 ASV.dial :wasteR @04:41:12.85 AS.reconfigure ASslow1 @04:41:12.90 AS.seek 0ml @04:44:34.29 delay 10 seconds @04:44:44.32 ATV.dial :air3 @04:44:45.83 AS.seek 0.01ml @04:44:48.13 delay 2 seconds @04:44:50.15 ATV.dial :bleach @04:44:50.77 AS.seek 0.58ml @04:46:45.65 delay 3 seconds @04:46:48.67 ATV.dial :air3 @04:46:49.23 delay 3 seconds @04:46:52.30 AS.seek 0.605ml @04:46:57.64 delay 3 seconds @04:47:00.66 ATV.dial :bleach @04:47:01.29 AS.seek 0.59ml @04:47:04.59 delay 3 seconds @04:47:07.61 ATV.dial ASV @04:47:08.66 ASV.dial :SPEdelivery @04:47:09.05 AS.seek 0ml @04:49:07.96 delay 1:00 @04:50:07.99 AFV.select :carrierSolution @04:50:08.10 delay 2 seconds @04:50:10.12 AS.reconfigure ASconfig @04:50:10.17 AS.seek 1ml @04:51:07.93 delay 1 second @04:51:08.95 AFV.select :holdingCoil @04:51:09.06 delay 1 second @04:51:10.07 ATV.dial ASV @04:51:10.08 ASV.dial :SPEdelivery @04:51:10.10 AS.reconfigure ASslow1 @04:51:10.15 AS.seek 0ml @04:54:31.51 delay 10 seconds @04:54:41.54 ATV.dial :air2 @04:54:42.38 AS.reconfigure ASslow0 @04:54:42.42 AS.seek 0.8ml @04:56:03.21 delay 3 seconds @04:56:06.23 ATV.dial :ASV @04:56:07.23 ASV.dial :SPEdelivery @04:56:07.25 AS.reconfigure ASslow1 @04:56:07.29 AS.seek 0.03ml @04:58:42.41 delay 20 seconds @04:59:02.44 ATV.dial :waste,via: :air3 @04:59:04.18 AS.reconfigure ASconfig @04:59:04.23 AS.seek :empty @04:59:06.29 SPE.coast @04:59:06.36 Removing Bleach from Mixing Coil @04:59:06.37 AFV.select :carrierSolution @04:59:06.47 delay 2 seconds @04:59:08.49 AS.seek 0.5ml @04:59:37.59 delay 1 second @04:59:38.61 AFV.select :holdingCoil @04:59:38.71 ATV.dial :ASV @04:59:40.40 ASV.dial :SPErecovery @04:59:41.04 AS.reconfigure ASslow1 @04:59:41.08 AS.seek 0.73ml @05:00:27.59 ASV.dial :wasteD @05:00:28.17 AS.reconfigure ASnorm @05:00:28.21 AS.seek 0ml @05:01:05.32 AFV.select :carrierSolution @05:01:05.43 delay 2 seconds @05:01:07.45 AS.reconfigure ASconfig @05:01:07.50 AS.seek 0.5ml @05:01:36.54 delay 1 second @05:01:37.56 AFV.select :holdingCoil @05:01:37.66 ATV.dial ASV @05:01:37.68 ASV.dial :SPErecovery @05:01:38.32 AS.reconfigure ASslow0 @05:01:38.36 AS.seek 0.3ml @05:01:58.82 ATV.dial :air2 @05:01:59.85 AS.reconfigure ASslow1 @05:01:59.89 AS.seek 0.32ml @05:02:04.25 ATV.dial ASV @05:02:05.27 AS.reconfigure ASslow0 @05:02:05.31 AS.seek 0.57ml @05:02:30.76 ASV.dial :wasteD @05:02:31.35 AS.reconfigure ASnorm @05:02:31.39 AS.seek 0ml @05:03:00.45 AFV.select :carrierSolution @05:03:00.56 delay 2 seconds @05:03:02.58 AS.reconfigure ASconfig @05:03:02.63 AS.seek 0.5ml @05:03:31.70 delay 1 second @05:03:32.72 AFV.select :holdingCoil @05:03:32.82 ATV.dial ASV @05:03:32.84 ASV.dial :SPErecovery @05:03:33.47 AS.reconfigure ASslow0 @05:03:33.51 AS.seek 0.3ml @05:03:53.93 ATV.dial :air2 @05:03:54.96 AS.reconfigure ASslow1 @05:03:55.00 AS.seek 0.32ml @05:03:59.38 ATV.dial ASV @05:04:00.41 AS.reconfigure ASslow0 @05:04:00.97 AS.seek 0.57ml @05:04:26.46 ASV.dial :wasteD @05:04:27.05 AS.reconfigure ASnorm @05:04:27.09 AS.seek 0ml @05:04:56.15 ATV.dial :air2 @05:04:57.13 AS.reconfigure ASslow0 @05:04:57.17 AS.seek 0.3ml @05:05:27.68 ATV.dial :ASV @05:05:28.65 ASV.dial :wasteD @05:05:28.67 AS.reconfigure ASconfig @05:05:28.71 AS.seek 0.05ml @05:05:43.40 ATV.dial :waste @05:05:45.27 AS.seek 0ml @05:05:48.46 ASV.dial :SPEdelivery @05:05:49.45 deconLysateINT @05:05:49.46 ATV.dial :air3 @05:05:50.13 AS.reconfigure ASslow1 @05:05:50.18 AS.seek 0.01ml @05:05:52.51 delay 2 seconds @05:05:54.53 ATV.dial :bleach @05:05:54.96 AS.seek 0.96ml @05:09:06.24 delay 3 seconds @05:09:09.26 ATV.dial :air3 @05:09:09.81 delay 3 seconds @05:09:12.88 AS.seek 0.985ml @05:09:18.16 delay 3 seconds @05:09:21.18 ATV.dial :bleach @05:09:21.79 AS.seek 0.97ml @05:09:25.12 delay 3 seconds @05:09:28.14 ATV.dial AIV @05:09:29.38 AIV.dial :coilToMFB @05:09:29.39 AWV.select :waste @05:09:29.40 AFV.select :carrierSolution @05:09:29.51 delay 2 seconds @05:09:31.53 AS.reconfigure ASconfig @05:09:31.58 AS.seek 1ml @05:09:33.60 delay 1 second @05:09:34.62 AFV.select :holdingCoil @05:09:34.73 delay 1 second @05:09:35.75 AS.reconfigure ASslow0 @05:09:35.79 AS.seek :empty @05:11:16.71 AFV.select :carrierSolution @05:11:16.82 delay 2 seconds @05:11:18.84 AS.reconfigure ASconfig @05:11:18.89 AS.seek 1ml @05:12:16.68 delay 1 second @05:12:17.70 AFV.select :holdingCoil @05:12:17.81 delay 1 second @05:12:18.83 AS.reconfigure ASslow0 @05:12:18.87 AS.seek :empty @05:13:59.85 AFV.select :carrierSolution @05:13:59.96 delay 2 seconds @05:14:01.98 AS.reconfigure ASconfig @05:14:02.03 AS.seek 1ml @05:14:59.82 delay 1 second @05:15:00.84 AFV.select :holdingCoil @05:15:00.95 delay 1 second @05:15:01.97 AS.reconfigure ASslow0 @05:15:02.01 AS.seek :empty @05:16:42.96 ATV.dial :air2 @05:16:43.62 AS.reconfigure ASslow1 @05:16:43.66 AS.seek 0.009ml @05:16:45.98 AS.reconfigure ASslow0 @05:16:46.03 AS.seek 1ml @05:18:25.96 ATV.dial AIV @05:18:26.74 AS.seek 0.02ml @05:20:05.62 ATV.dial :waste @05:20:07.29 AS.seek 0ml @05:20:09.59 AWV.select :air @05:20:09.74 Cleaning PCR @05:20:09.75 AFV.select :carrierSolution @05:20:09.85 delay 2 seconds @05:20:11.87 AS.reconfigure ASconfig @05:20:11.92 AS.seek 0.5ml @05:20:40.96 delay 1 second @05:20:41.98 AFV.select :holdingCoil @05:20:42.08 ASV.dial :waste1 @05:20:42.46 ATV.dial :air3 @05:20:43.13 AS.reconfigure ASslow1 @05:20:43.17 AS.seek 0.51ml @05:20:45.43 delay 2 seconds @05:20:47.45 ATV.dial :bleach @05:20:47.90 AS.seek 0.81ml @05:21:48.48 delay 3 seconds @05:21:51.50 ATV.dial :air3 @05:21:52.06 delay 3 seconds @05:21:55.13 AS.seek 0.835ml @05:22:00.42 delay 3 seconds @05:22:03.44 ATV.dial :bleach @05:22:04.05 AS.seek 0.82ml @05:22:07.37 delay 3 seconds @05:22:10.87 ARV.dial :waste @05:22:11.51 delay 3 seconds @05:22:14.53 ATV.dial :ARV @05:22:15.40 AS.reconfigure ASconfig @05:22:15.44 AS.seek 0.47ml @05:22:35.85 AFV.select :carrierSolution @05:22:35.96 delay 2 seconds @05:22:37.98 AS.seek 1ml @05:23:08.76 delay 1 second @05:23:09.78 AFV.select :holdingCoil @05:23:09.89 delay 1 second @05:23:10.91 AS.reconfigure ASfast @05:23:10.96 AS.seek 0ml @05:23:38.31 AFV.select :carrierSolution @05:23:38.42 delay 2 seconds @05:23:40.44 AS.reconfigure ASconfig @05:23:40.49 AS.seek 1ml @05:24:38.26 delay 1 second @05:24:39.28 AFV.select :holdingCoil @05:24:39.39 delay 1 second @05:24:40.41 AS.reconfigure ASfast @05:24:40.46 AS.seek 0ml @05:25:07.81 AFV.select :carrierSolution @05:25:07.92 delay 2 seconds @05:25:09.94 AS.reconfigure ASconfig @05:25:09.99 AS.seek 1ml @05:26:07.78 delay 1 second @05:26:08.80 AFV.select :holdingCoil @05:26:08.91 delay 1 second @05:26:09.93 AS.reconfigure ASfast @05:26:09.98 AS.seek 0ml @05:26:37.32 ARV.dial between carrier and air1 @05:26:37.82 ATV.dial :air3 @05:26:39.10 AS.reconfigure ASslow1 @05:26:39.14 AS.seek 0.01ml @05:26:41.42 delay 2 seconds @05:26:43.44 ATV.dial :bleach @05:26:44.05 AS.seek 0.81ml @05:29:25.20 delay 3 seconds @05:29:28.22 ATV.dial :air3 @05:29:28.78 delay 3 seconds @05:29:31.85 AS.seek 0.835ml @05:29:37.15 delay 3 seconds @05:29:40.17 ATV.dial :bleach @05:29:40.79 AS.seek 0.82ml @05:29:44.03 delay 3 seconds @05:29:47.05 ATV.dial PCR @05:29:47.45 AS.reconfigure ASconfig @05:29:47.50 AS.seek :empty @05:30:34.98 PCR.program :bleachProfile @05:30:35.03 PCR program should complete in < 2:45 @05:30:35.04 PCR.run 10:00 @05:33:36.85 PCR.off @05:33:36.88 AFV.select :carrierSolution @05:33:36.99 delay 2 seconds @05:33:39.01 AS.seek 1ml @05:34:36.81 delay 1 second @05:34:37.83 AFV.select :holdingCoil @05:34:37.94 delay 1 second @05:34:38.96 AS.reconfigure ASfast @05:34:39.01 AS.seek 0ml @05:35:06.34 delay 3 seconds @05:35:09.36 AFV.select :carrierSolution @05:35:09.47 delay 2 seconds @05:35:11.49 AS.reconfigure ASconfig @05:35:11.54 AS.seek 1ml @05:36:09.28 delay 1 second @05:36:10.30 AFV.select :holdingCoil @05:36:10.41 delay 1 second @05:36:11.43 AS.reconfigure ASfast @05:36:11.48 AS.seek 0ml @05:36:38.82 delay 3 seconds @05:36:41.84 AFV.select :carrierSolution @05:36:41.95 delay 2 seconds @05:36:43.97 AS.reconfigure ASconfig @05:36:44.03 AS.seek 1ml @05:37:41.86 delay 1 second @05:37:42.88 AFV.select :holdingCoil @05:37:42.99 delay 1 second @05:37:44.01 AS.reconfigure ASfast @05:37:44.06 AS.seek 0ml @05:38:11.48 delay 3 seconds @05:38:14.50 AFV.select :carrierSolution @05:38:14.61 delay 2 seconds @05:38:16.63 AS.reconfigure ASconfig @05:38:16.68 AS.seek 1ml @05:39:14.48 delay 1 second @05:39:15.50 AFV.select :holdingCoil @05:39:15.61 delay 1 second @05:39:16.63 AS.reconfigure ASfast @05:39:16.68 AS.seek 0ml @05:39:44.03 delay 3 seconds @05:39:47.05 AFV.select :carrierSolution @05:39:47.16 delay 2 seconds @05:39:49.18 AS.reconfigure ASconfig @05:39:49.24 AS.seek 1ml @05:40:47.01 delay 1 second @05:40:48.03 AFV.select :holdingCoil @05:40:48.14 delay 1 second @05:40:49.16 AS.reconfigure ASnorm @05:40:49.21 AS.seek 0ml @05:41:40.41 AFV.select :carrierSolution @05:41:40.52 delay 2 seconds @05:41:42.54 AS.reconfigure ASconfig @05:41:42.59 AS.seek 1ml @05:42:40.37 delay 1 second @05:42:41.39 AFV.select :holdingCoil @05:42:41.50 delay 1 second @05:42:42.51 ATV.dial :waste @05:42:43.74 AS.reconfigure ASnorm @05:42:43.78 AS.seek 0.5ml @05:43:09.34 ATV.dial :waste2 @05:43:10.62 AS.seek 0ml @05:43:36.15
Asleep after completing 18tun_postDeploy3 assays @05:43:36.16 GoodNews.email "{3 => 3, 4 => 6}\nCan@05:41:38, 19.1C, 37% humidity, 14.6psia, 12.046V, 0.487A, 0.487A avg, 5.87W",Subject: "Asleep after completing 18tun_postDeploy3 assays" @05:43:36.19
Mission runtime was 13:11:59.605173 @14:09:12.35CET10-Dec-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @14:09:14.00 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @14:09:14.25 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @14:09:18.49 Polling Can every 10:00 @14:09:20.24 SPE.coast @14:09:20.29 SPE.configure SPEconfig @14:09:20.37 AS.reconfigure ASconfig @14:09:20.41 AS.hold @14:09:20.47 AS.configure ASconfig @14:09:20.55 SS.reconfigure SSconfig @14:09:20.59 SS.hold @14:09:20.65 SS.configure SSconfig @14:09:20.73 CC.reconfigure CCconfig @14:09:20.77 CC.hold @14:09:20.85 CC.configure CCconfig @14:09:20.92 CS.reconfigure CSconfig @14:09:20.97 CS.hold @14:09:21.03 CS.configure CSconfig @14:09:21.45 PC.reconfigure PCconfig @14:09:21.49 PC.hold @14:09:21.57 PC.configure PCconfig @14:09:21.65 PS.configure PSconfig @14:09:21.74 Forearm.reconfigure LooseForearm @14:09:21.78 Forearm.hold @14:09:21.84 Forearm.configure LooseForearm @14:09:21.92 Elbow.reconfigure LooseElbow @14:09:21.95 Elbow.hold @14:09:22.02 Elbow.configure LooseElbow @14:09:22.11 SC.reconfigure SCloose @14:09:22.15 SC.hold @14:09:22.21 SC.configure SCloose @14:09:22.29 SE.reconfigure SEconfig @14:09:22.33 SE.hold @14:09:22.39 SE.configure SEconfig @14:09:22.42 CH.coast @14:09:22.47 CH.configure CHconfig @14:09:22.50 PH.coast @14:09:22.54 PH.configure PHconfig @14:09:22.58 PCR.off @14:09:22.61 PCR.pidtemp= 50,0.1,0,300,25,25 @14:09:22.64 PCR.caltemp= 2.7073,0.0373,0 @14:09:22.67 All dwarf controllers running firmware version 3.75 @14:09:22.78 Cleanly exited at 13:59:57CET10-Dec-18 @14:09:22.81 Loading /home/esp/esp2/mission/stdMER2017_MWJ.rb mission @14:09:23.35 AFV.select :carrierSolution @14:09:23.44 delay 2 seconds @14:09:25.47 AS.seek 0.5ml @14:09:33.90 Session Begun @14:09:54.53
delay 1 second @14:09:55.55 AFV.select :holdingCoil @14:09:55.65 ATV.dial :extSrc @14:09:56.11 AS.reconfigure ASslow1 @14:09:56.17 AS.seek 0.53ml @14:10:02.48 delay 5 seconds @14:10:07.56 AS.reconfigure ASslow2 @14:10:07.62 AS.seek 0.54ml @14:10:17.91 delay 20 seconds @14:10:37.98 AS.reconfigure ASslow1 @14:10:38.04 AS.seek 0.57ml @14:10:44.39 delay 5 seconds @14:10:49.47 AS.reconfigure ASslow2 @14:10:49.53 AS.seek 0.58ml @14:10:59.83 delay 20 seconds @14:11:19.90 AS.reconfigure ASslow1 @14:11:19.96 AS.seek 0.61ml @14:11:26.23 delay 5 seconds @14:11:31.31 AS.reconfigure ASslow2 @14:11:31.37 AS.seek 0.62ml @14:11:41.69 delay 20 seconds @14:12:01.76 AS.reconfigure ASslow1 @14:12:01.82 AS.seek 0.65ml @14:12:08.17 delay 5 seconds @14:12:13.25 AS.reconfigure ASslow2 @14:12:13.31 AS.seek 0.66ml @14:12:23.64 delay 20 seconds @14:12:43.68 AS.reconfigure ASslow1 @14:12:43.73 AS.seek 0.705ml @14:12:53.05 delay 10 seconds @14:13:03.07 ATV.dial :air1 @14:13:04.79 AS.seek 0.725ml @14:13:09.08 delay 3 seconds @14:13:12.10 ATV.dial :waste @14:13:12.67 AS.reconfigure ASslow0 @14:13:12.73 AS.seek :empty @14:14:26.20 Setting up PCR for Bb -- writing /var/log/esp/Bb18dec1014h.pcr @14:14:26.22 Building PCR Slug from extSrc with MM3 mix, PP5 primer @14:14:26.25 Priming with carrier @14:14:26.27 ATV.dial ARV @14:14:27.87 ARV.dial :carrier @14:14:28.23 AS.reconfigure ASnorm @14:14:28.29 AS.seek 0.499ml @14:14:53.83 AFV.select :carrierSolution @14:14:53.94 delay 2 seconds @14:14:55.97 AS.reconfigure ASconfig @14:14:56.03 AS.seek 1ml @14:15:25.11 delay 1 second @14:15:26.13 AFV.select :holdingCoil @14:15:26.23 delay 1 second @14:15:27.25 ATV.dial :waste @14:15:28.91 AS.reconfigure ASnorm @14:15:28.97 AS.seek :empty @14:16:19.75 AFV.select :carrierSolution @14:16:19.86 delay 2 seconds @14:16:21.89 AS.reconfigure ASconfig @14:16:21.95 AS.seek 0.5ml @14:16:51.08 delay 1 second @14:16:52.10 AFV.select :holdingCoil @14:16:52.20 Building Slug @14:16:52.21 ATV.dial :ARV @14:16:53.87 delay 2 seconds @14:16:55.89 ARV.dial :air1 @14:16:56.30 delay 2 seconds @14:16:58.37 AS.reconfigure ASslow2 @14:16:58.43 AS.seek 0.506ml @14:17:04.76 delay 2 seconds @14:17:06.78 ARV.dial :MM3 @14:17:07.87 delay 2 seconds @14:17:09.89 Adding MM3 @14:17:09.98 AS.seek 0.524ml @14:17:28.28 delay 25 seconds @14:17:53.30 ARV.dial :PP5 @14:17:53.70 Adding PP5 @14:17:53.71 delay 2 seconds @14:17:55.79 AS.seek 0.53ml @14:18:02.25 delay 10 seconds @14:18:12.28 ARV.dial :air3 @14:18:12.69 adding ARV air @14:18:12.70 delay 2 seconds @14:18:14.78 AS.seek 0.536ml @14:18:21.06 delay 5 seconds @14:18:26.08 ARV.dial :carrier @14:18:27.39 delay 7 seconds @14:18:34.47 AS.seek 0.594ml @14:19:32.92 delay 5 seconds @14:19:37.94 ATV.dial :extSrc @14:19:38.50 delay 2 seconds @14:19:40.57 AS.seek 0.6ml @14:19:46.86 delay 4 seconds @14:19:50.88 ATV.dial ARV @14:19:51.44 delay 2 seconds @14:19:53.46 ARV.dial :carrier @14:19:53.47 delay 2 seconds @14:19:55.55 AS.seek 0.65ml @14:20:46.26 delay 4 seconds @14:20:50.28 ATV.dial PCR @14:20:51.06 ASV.dial :waste1 @14:20:51.16 ARV.dial :air1 @14:20:51.98 Positioning Slug by 0.324ml @14:20:52.08 AS.seek 0.326ml @14:26:18.14 delay 5 seconds @14:26:23.16 ATV.dial ARV @14:26:23.95 ASV.dial :PCRlock @14:26:24.36 delay 5 seconds @14:26:29.38 Running Bb Profile60C300 thermocycling @14:26:29.40 PCR.program Profile60C300 @14:26:29.54 Profile60C300 should complete in < 1:45:21 @14:26:29.55 PCR.run 2:16:41.25 @15:48:53.96 PCR.off @15:48:55.48 PCR for Bb completed -- closed /var/log/esp/Bb18dec1014h.pcr @15:48:55.56 Cleaning PCR @15:48:55.57 AFV.select :carrierSolution @15:48:55.69 delay 2 seconds @15:48:57.72 AS.reconfigure ASconfig @15:48:57.77 AS.seek 0.5ml @15:49:08.05 delay 1 second @15:49:09.07 AFV.select :holdingCoil @15:49:09.18 ASV.dial :waste1 @15:49:09.58 ATV.dial :air3 @15:49:10.86 AS.reconfigure ASslow1 @15:49:10.92 AS.seek 0.51ml @15:49:13.12 delay 2 seconds @15:49:15.14 ATV.dial :bleach @15:49:15.77 AS.seek 0.81ml @15:50:16.38 delay 3 seconds @15:50:19.40 ATV.dial :air3 @15:50:19.96 delay 3 seconds @15:50:23.03 AS.seek 0.835ml @15:50:28.33 delay 3 seconds @15:50:31.35 ATV.dial :bleach @15:50:31.97 AS.seek 0.82ml @15:50:35.32 delay 3 seconds @15:50:38.34 ARV.dial :waste @15:50:38.97 delay 3 seconds @15:50:41.99 ATV.dial :ARV @15:50:42.88 AS.reconfigure ASconfig @15:50:42.94 AS.seek 0.47ml @15:51:03.38 AFV.select :carrierSolution @15:51:03.49 delay 2 seconds @15:51:05.52 AS.seek 1ml @15:51:36.32 delay 1 second @15:51:37.34 AFV.select :holdingCoil @15:51:37.44 delay 1 second @15:51:38.47 AS.reconfigure ASfast @15:51:38.52 AS.seek 0ml @15:52:05.89 AFV.select :carrierSolution @15:52:06.00 delay 2 seconds @15:52:08.03 AS.reconfigure ASconfig @15:52:08.08 AS.seek 1ml @15:53:05.92 delay 1 second @15:53:06.94 AFV.select :holdingCoil @15:53:07.05 delay 1 second @15:53:08.41 AS.reconfigure ASfast @15:53:08.53 AS.seek 0ml @15:53:35.91 AFV.select :carrierSolution @15:53:36.02 delay 2 seconds @15:53:38.05 AS.reconfigure ASconfig @15:53:38.10 AS.seek 1ml @15:54:35.88 delay 1 second @15:54:36.90 AFV.select :holdingCoil @15:54:37.00 delay 1 second @15:54:38.03 AS.reconfigure ASfast @15:54:38.08 AS.seek 0ml @15:55:05.44 ARV.dial between carrier and air1 @15:55:05.96 ATV.dial :air3 @15:55:07.27 AS.reconfigure ASslow1 @15:55:07.33 AS.seek 0.01ml @15:55:09.69 delay 2 seconds @15:55:11.71 ATV.dial :bleach @15:55:12.34 AS.seek 0.81ml @15:57:53.49 delay 3 seconds @15:57:56.51 ATV.dial :air3 @15:57:57.08 delay 3 seconds @15:58:00.15 AS.seek 0.835ml @15:58:05.51 delay 3 seconds @15:58:08.53 ATV.dial :bleach @15:58:09.16 AS.seek 0.82ml @15:58:12.47 delay 3 seconds @15:58:15.49 ATV.dial PCR @15:58:15.89 AS.reconfigure ASconfig @15:58:15.95 AS.seek :empty @15:59:03.46 PCR.program :bleachProfile @15:59:03.51 PCR program should complete in < 2:45 @15:59:03.53 PCR.run 10:00 @16:01:59.31 PCR.off @16:01:59.34 AFV.select :carrierSolution @16:01:59.46 delay 2 seconds @16:02:01.49 AS.seek 1ml @16:02:59.32 delay 1 second @16:03:00.34 AFV.select :holdingCoil @16:03:00.45 delay 1 second @16:03:01.48 AS.reconfigure ASfast @16:03:01.53 AS.seek 0ml @16:03:28.89 delay 3 seconds @16:03:31.91 AFV.select :carrierSolution @16:03:32.01 delay 2 seconds @16:03:34.04 AS.reconfigure ASconfig @16:03:34.10 AS.seek 1ml @16:04:31.89 delay 1 second @16:04:32.91 AFV.select :holdingCoil @16:04:33.01 delay 1 second @16:04:34.04 AS.reconfigure ASfast @16:04:34.09 AS.seek 0ml @16:05:01.83 delay 3 seconds @16:05:04.85 AFV.select :carrierSolution @16:05:04.95 delay 2 seconds @16:05:06.98 AS.reconfigure ASconfig @16:05:07.04 AS.seek 1ml @16:06:04.82 delay 1 second @16:06:05.84 AFV.select :holdingCoil @16:06:05.95 delay 1 second @16:06:06.98 AS.reconfigure ASfast @16:06:07.03 AS.seek 0ml @16:06:34.41 delay 3 seconds @16:06:37.43 AFV.select :carrierSolution @16:06:37.53 delay 2 seconds @16:06:39.56 AS.reconfigure ASconfig @16:06:39.62 AS.seek 1ml @16:07:37.38 delay 1 second @16:07:38.40 AFV.select :holdingCoil @16:07:38.51 delay 1 second @16:07:39.54 AS.reconfigure ASfast @16:07:39.59 AS.seek 0ml @16:08:06.96 delay 3 seconds @16:08:09.98 AFV.select :carrierSolution @16:08:10.09 delay 2 seconds @16:08:12.12 AS.reconfigure ASconfig @16:08:12.18 AS.seek 1ml @16:09:09.96 delay 1 second @16:09:10.98 AFV.select :holdingCoil @16:09:11.08 delay 1 second @16:09:12.11 AS.reconfigure ASnorm @16:09:12.16 AS.seek 0ml @16:10:02.89
AFV.select :carrierSolution @16:10:03.00 delay 2 seconds @16:10:05.03 AS.reconfigure ASconfig @16:10:05.08 AS.seek 0.5ml @16:10:34.14 delay 1 second @16:10:35.16 AFV.select :holdingCoil @16:10:35.26 ATV.dial :air1 @16:10:36.77 AS.reconfigure ASslow2 @16:10:36.83 AS.seek 0.506ml @16:10:43.19 ATV.dial :extSrc @16:10:44.89 AS.seek 0.512ml @16:10:51.28 delay 5 seconds @16:10:56.30 ATV.dial :air1 @16:10:58.01 AS.reconfigure ASslow1 @16:10:58.07 AS.seek 0.542ml @16:11:04.39 ATV.dial :waste @16:11:04.96 AS.reconfigure ASconfig @16:11:05.02 AS.seek :empty @16:11:36.69 Setting up PCR for Bb -- writing /var/log/esp/Bb18dec1016h.pcr @16:11:36.71 Building PCR Slug from extSrc with MM3 mix, PP5 primer @16:11:36.75 Priming with carrier @16:11:36.77 ATV.dial ARV @16:11:38.27 ARV.dial :carrier @16:11:38.63 AS.reconfigure ASnorm @16:11:38.69 AS.seek 0.499ml @16:12:04.28 AFV.select :carrierSolution @16:12:04.39 delay 2 seconds @16:12:06.42 AS.reconfigure ASconfig @16:12:06.48 AS.seek 1ml @16:12:35.57 delay 1 second @16:12:36.59 AFV.select :holdingCoil @16:12:36.69 delay 1 second @16:12:37.71 ATV.dial :waste @16:12:39.38 AS.reconfigure ASnorm @16:12:39.45 AS.seek :empty @16:13:30.21 AFV.select :carrierSolution @16:13:30.32 delay 2 seconds @16:13:32.35 AS.reconfigure ASconfig @16:13:32.40 AS.seek 0.5ml @16:14:01.52 delay 1 second @16:14:02.54 AFV.select :holdingCoil @16:14:02.64 Building Slug @16:14:02.65 ATV.dial :ARV @16:14:04.30 delay 2 seconds @16:14:06.32 ARV.dial :air1 @16:14:06.73 delay 2 seconds @16:14:08.80 AS.reconfigure ASslow2 @16:14:08.86 AS.seek 0.506ml @16:14:15.21 delay 2 seconds @16:14:17.23 ARV.dial :MM3 @16:14:18.33 delay 2 seconds @16:14:20.35 Adding MM3 @16:14:20.44 AS.seek 0.524ml @16:14:39.33 delay 25 seconds @16:15:04.35 ARV.dial :PP5 @16:15:04.75 Adding PP5 @16:15:04.76 delay 2 seconds @16:15:06.84 AS.seek 0.53ml @16:15:13.07 delay 10 seconds @16:15:23.10 ARV.dial :air3 @16:15:23.50 adding ARV air @16:15:23.51 delay 2 seconds @16:15:25.59 AS.seek 0.536ml @16:15:31.83 delay 5 seconds @16:15:36.85 ARV.dial :carrier @16:15:38.16 delay 7 seconds @16:15:45.24 AS.seek 0.594ml @16:16:43.79 delay 5 seconds @16:16:48.81 ATV.dial :extSrc @16:16:49.38 delay 2 seconds @16:16:51.45 AS.seek 0.6ml @16:16:57.69 delay 4 seconds @16:17:01.71 ATV.dial ARV @16:17:02.27 delay 2 seconds @16:17:04.29 ARV.dial :carrier @16:17:04.30 delay 2 seconds @16:17:06.38 AS.seek 0.65ml @16:17:56.86 delay 4 seconds @16:18:00.88 ATV.dial PCR @16:18:01.66 ASV.dial :waste1 @16:18:01.67 ARV.dial :air1 @16:18:02.11 Positioning Slug by 0.324ml @16:18:02.20 AS.seek 0.326ml @16:23:28.25 delay 5 seconds @16:23:33.27 ATV.dial ARV @16:23:34.16 ASV.dial :PCRlock @16:23:34.57 delay 5 seconds @16:23:39.59 Running Bb Profile60C300 thermocycling @16:23:39.60 PCR.program Profile60C300 @16:23:39.74 Profile60C300 should complete in < 1:45:21 @16:23:39.75 PCR.run 2:16:41.25 @17:45:48.86 PCR.off @17:45:50.36 PCR for Bb completed -- closed /var/log/esp/Bb18dec1016h.pcr @17:45:50.43 Cleaning PCR @17:45:50.45 AFV.select :carrierSolution @17:45:50.57 delay 2 seconds @17:45:52.60 AS.reconfigure ASconfig @17:45:52.65 AS.seek 0.5ml @17:46:02.95 delay 1 second @17:46:03.97 AFV.select :holdingCoil @17:46:04.08 ASV.dial :waste1 @17:46:04.48 ATV.dial :air3 @17:46:06.16 AS.reconfigure ASslow1 @17:46:06.22 AS.seek 0.51ml @17:46:08.51 delay 2 seconds @17:46:10.53 ATV.dial :bleach @17:46:11.16 AS.seek 0.81ml @17:47:11.76 delay 3 seconds @17:47:14.78 ATV.dial :air3 @17:47:15.34 delay 3 seconds @17:47:18.41 AS.seek 0.835ml @17:47:23.72 delay 3 seconds @17:47:26.74 ATV.dial :bleach @17:47:27.36 AS.seek 0.82ml @17:47:30.66 delay 3 seconds @17:47:33.68 ARV.dial :waste @17:47:34.30 delay 3 seconds @17:47:37.32 ATV.dial :ARV @17:47:38.22 AS.reconfigure ASconfig @17:47:38.28 AS.seek 0.47ml @17:47:58.70 AFV.select :carrierSolution @17:47:58.81 delay 2 seconds @17:48:00.84 AS.seek 1ml @17:48:31.63 delay 1 second @17:48:32.65 AFV.select :holdingCoil @17:48:32.75 delay 1 second @17:48:33.78 AS.reconfigure ASfast @17:48:33.83 AS.seek 0ml @17:49:01.20 AFV.select :carrierSolution @17:49:01.31 delay 2 seconds @17:49:03.34 AS.reconfigure ASconfig @17:49:03.39 AS.seek 1ml @17:50:01.18 delay 1 second @17:50:02.20 AFV.select :holdingCoil @17:50:02.30 delay 1 second @17:50:03.33 AS.reconfigure ASfast @17:50:03.38 AS.seek 0ml @17:50:30.74 AFV.select :carrierSolution @17:50:30.85 delay 2 seconds @17:50:32.88 AS.reconfigure ASconfig @17:50:32.93 AS.seek 1ml @17:51:30.77 delay 1 second @17:51:31.79 AFV.select :holdingCoil @17:51:31.90 delay 1 second @17:51:32.93 AS.reconfigure ASfast @17:51:32.98 AS.seek 0ml @17:52:00.35 ARV.dial between carrier and air1 @17:52:00.87 ATV.dial :air3 @17:52:02.16 AS.reconfigure ASslow1 @17:52:02.22 AS.seek 0.01ml @17:52:04.52 delay 2 seconds @17:52:06.54 ATV.dial :bleach @17:52:07.18 AS.seek 0.81ml @17:54:48.35 delay 3 seconds @17:54:51.37 ATV.dial :air3 @17:54:51.95 delay 3 seconds @17:54:55.02 AS.seek 0.835ml @17:55:00.45 delay 3 seconds @17:55:03.47 ATV.dial :bleach @17:55:04.12 AS.seek 0.82ml @17:55:07.37 delay 3 seconds @17:55:10.39 ATV.dial PCR @17:55:10.79 AS.reconfigure ASconfig @17:55:10.85 AS.seek :empty @17:55:58.34 PCR.program :bleachProfile @17:55:58.40 PCR program should complete in < 2:45 @17:55:58.41 PCR.run 10:00 @17:58:57.22 PCR.off @17:58:57.25 AFV.select :carrierSolution @17:58:57.36 delay 2 seconds @17:58:59.39 AS.seek 1ml @17:59:57.27 delay 1 second @17:59:58.29 AFV.select :holdingCoil @17:59:58.40 delay 1 second @17:59:59.43 AS.reconfigure ASfast @17:59:59.48 AS.seek 0ml @18:00:26.85 delay 3 seconds @18:00:29.87 AFV.select :carrierSolution @18:00:29.97 delay 2 seconds @18:00:32.00 AS.reconfigure ASconfig @18:00:32.06 AS.seek 1ml @18:01:29.85 delay 1 second @18:01:30.87 AFV.select :holdingCoil @18:01:30.98 delay 1 second @18:01:32.01 AS.reconfigure ASfast @18:01:32.06 AS.seek 0ml @18:01:59.41 delay 3 seconds @18:02:02.43 AFV.select :carrierSolution @18:02:02.53 delay 2 seconds @18:02:04.56 AS.reconfigure ASconfig @18:02:04.62 AS.seek 1ml @18:03:02.38 delay 1 second @18:03:03.40 AFV.select :holdingCoil @18:03:03.50 delay 1 second @18:03:04.53 AS.reconfigure ASfast @18:03:04.58 AS.seek 0ml @18:03:31.93 delay 3 seconds @18:03:34.95 AFV.select :carrierSolution @18:03:35.05 delay 2 seconds @18:03:37.08 AS.reconfigure ASconfig @18:03:37.14 AS.seek 1ml @18:04:34.91 delay 1 second @18:04:35.93 AFV.select :holdingCoil @18:04:36.04 delay 1 second @18:04:37.07 AS.reconfigure ASfast @18:04:37.12 AS.seek 0ml @18:05:04.51 delay 3 seconds @18:05:07.53 AFV.select :carrierSolution @18:05:07.64 delay 2 seconds @18:05:09.67 AS.reconfigure ASconfig @18:05:09.73 AS.seek 1ml @18:06:07.52 delay 1 second @18:06:08.54 AFV.select :holdingCoil @18:06:08.65 delay 1 second @18:06:09.68 AS.reconfigure ASnorm @18:06:09.73 AS.seek 0ml @18:07:00.46
AFV.select :carrierSolution @18:07:00.57 delay 2 seconds @18:07:02.60 AS.reconfigure ASconfig @18:07:02.65 AS.seek 0.5ml @18:07:31.73 delay 1 second @18:07:32.75 AFV.select :holdingCoil @18:07:32.86 ATV.dial :air1 @18:07:34.37 AS.reconfigure ASslow2 @18:07:34.43 AS.seek 0.51ml @18:07:44.65 delay 5 seconds @18:07:49.67 ATV.dial :extSrc @18:07:51.49 AS.seek 0.53ml @18:08:11.76 delay 10 seconds @18:08:21.78 ATV.dial :air1 @18:08:23.48 AS.reconfigure ASslow1 @18:08:23.54 AS.seek 0.56ml @18:08:29.90 ATV.dial :waste @18:08:30.85 AS.reconfigure ASconfig @18:08:30.91 AS.seek :empty @18:09:03.58 Setting up PCR for Bb -- writing /var/log/esp/Bb18dec1018h.pcr @18:09:03.60 Building PCR Slug from extSrc with MM3 mix, PP5 primer @18:09:03.63 Priming with carrier @18:09:03.65 ATV.dial ARV @18:09:05.13 ARV.dial :carrier @18:09:05.49 AS.reconfigure ASnorm @18:09:05.55 AS.seek 0.499ml @18:09:31.10 AFV.select :carrierSolution @18:09:31.22 delay 2 seconds @18:09:33.25 AS.reconfigure ASconfig @18:09:33.31 AS.seek 1ml @18:10:02.40 delay 1 second @18:10:03.42 AFV.select :holdingCoil @18:10:03.52 delay 1 second @18:10:04.54 ATV.dial :waste @18:10:06.21 AS.reconfigure ASnorm @18:10:06.28 AS.seek :empty @18:10:57.02 AFV.select :carrierSolution @18:10:57.14 delay 2 seconds @18:10:59.17 AS.reconfigure ASconfig @18:10:59.23 AS.seek 0.5ml @18:11:28.37 delay 1 second @18:11:29.39 AFV.select :holdingCoil @18:11:29.49 Building Slug @18:11:29.50 ATV.dial :ARV @18:11:31.15 delay 2 seconds @18:11:33.17 ARV.dial :air1 @18:11:33.58 delay 2 seconds @18:11:35.65 AS.reconfigure ASslow2 @18:11:35.71 AS.seek 0.506ml @18:11:42.04 delay 2 seconds @18:11:44.06 ARV.dial :MM3 @18:11:45.13 delay 2 seconds @18:11:47.15 Adding MM3 @18:11:47.23 AS.seek 0.524ml @18:12:05.79 delay 25 seconds @18:12:30.81 ARV.dial :PP5 @18:12:31.21 Adding PP5 @18:12:31.22 delay 2 seconds @18:12:33.30 AS.seek 0.53ml @18:12:39.84 delay 10 seconds @18:12:49.87 ARV.dial :air3 @18:12:50.27 adding ARV air @18:12:50.28 delay 2 seconds @18:12:52.36 AS.seek 0.536ml @18:12:58.60 delay 5 seconds @18:13:03.62 ARV.dial :carrier @18:13:04.93 delay 7 seconds @18:13:12.01 AS.seek 0.594ml @18:14:10.56 delay 5 seconds @18:14:15.58 ATV.dial :extSrc @18:14:16.15 delay 2 seconds @18:14:18.22 AS.seek 0.6ml @18:14:24.67 delay 4 seconds @18:14:28.69 ATV.dial ARV @18:14:29.26 delay 2 seconds @18:14:31.28 ARV.dial :carrier @18:14:31.29 delay 2 seconds @18:14:33.37 AS.seek 0.65ml @18:15:24.02 delay 4 seconds @18:15:28.04 ATV.dial PCR @18:15:28.82 ASV.dial :waste1 @18:15:28.83 ARV.dial :air1 @18:15:29.26 Positioning Slug by 0.324ml @18:15:29.35 AS.seek 0.326ml @18:20:55.60 delay 5 seconds @18:21:00.62 ATV.dial ARV @18:21:01.42 ASV.dial :PCRlock @18:21:01.82 delay 5 seconds @18:21:06.84 Running Bb Profile60C300 thermocycling @18:21:06.85 PCR.program Profile60C300 @18:21:07.00 Profile60C300 should complete in < 1:45:21 @18:21:07.01 PCR.run 2:16:41.25 @19:43:17.99 PCR.off @19:43:19.51 PCR for Bb completed -- closed /var/log/esp/Bb18dec1018h.pcr @19:43:19.58 Cleaning PCR @19:43:19.59 AFV.select :carrierSolution @19:43:19.71 delay 2 seconds @19:43:21.74 AS.reconfigure ASconfig @19:43:21.79 AS.seek 0.5ml @19:43:32.09 delay 1 second @19:43:33.11 AFV.select :holdingCoil @19:43:33.21 ASV.dial :waste1 @19:43:33.61 ATV.dial :air3 @19:43:34.90 AS.reconfigure ASslow1 @19:43:34.96 AS.seek 0.51ml @19:43:37.23 delay 2 seconds @19:43:39.25 ATV.dial :bleach @19:43:39.87 AS.seek 0.81ml @19:44:40.47 delay 3 seconds @19:44:43.49 ATV.dial :air3 @19:44:44.05 delay 3 seconds @19:44:47.12 AS.seek 0.835ml @19:44:52.42 delay 3 seconds @19:44:55.44 ATV.dial :bleach @19:44:56.06 AS.seek 0.82ml @19:44:59.40 delay 3 seconds @19:45:02.42 ARV.dial :waste @19:45:03.05 delay 3 seconds @19:45:06.07 ATV.dial :ARV @19:45:06.95 AS.reconfigure ASconfig @19:45:07.01 AS.seek 0.47ml @19:45:27.44 AFV.select :carrierSolution @19:45:27.55 delay 2 seconds @19:45:29.58 AS.seek 1ml @19:46:00.34 delay 1 second @19:46:01.36 AFV.select :holdingCoil @19:46:01.46 delay 1 second @19:46:02.49 AS.reconfigure ASfast @19:46:02.54 AS.seek 0ml @19:46:29.90 AFV.select :carrierSolution @19:46:30.01 delay 2 seconds @19:46:32.04 AS.reconfigure ASconfig @19:46:32.09 AS.seek 1ml @19:47:29.87 delay 1 second @19:47:30.89 AFV.select :holdingCoil @19:47:30.99 delay 1 second @19:47:32.02 AS.reconfigure ASfast @19:47:32.07 AS.seek 0ml @19:47:59.45 AFV.select :carrierSolution @19:47:59.56 delay 2 seconds @19:48:01.59 AS.reconfigure ASconfig @19:48:01.64 AS.seek 1ml @19:48:59.44 delay 1 second @19:49:00.46 AFV.select :holdingCoil @19:49:00.56 delay 1 second @19:49:01.59 AS.reconfigure ASfast @19:49:01.64 AS.seek 0ml @19:49:29.00 ARV.dial between carrier and air1 @19:49:29.51 ATV.dial :air3 @19:49:30.80 AS.reconfigure ASslow1 @19:49:30.87 AS.seek 0.01ml @19:49:33.22 delay 2 seconds @19:49:35.24 ATV.dial :bleach @19:49:36.27 AS.seek 0.81ml @19:52:17.45 delay 3 seconds @19:52:20.47 ATV.dial :air3 @19:52:21.05 delay 3 seconds @19:52:24.12 AS.seek 0.835ml @19:52:29.48 delay 3 seconds @19:52:32.50 ATV.dial :bleach @19:52:33.12 AS.seek 0.82ml @19:52:36.36 delay 3 seconds @19:52:39.38 ATV.dial PCR @19:52:39.78 AS.reconfigure ASconfig @19:52:39.84 AS.seek :empty @19:53:27.31 PCR.program :bleachProfile @19:53:27.36 PCR program should complete in < 2:45 @19:53:27.37 PCR.run 10:00 @19:56:26.17 PCR.off @19:56:26.20 AFV.select :carrierSolution @19:56:26.32 delay 2 seconds @19:56:28.35 AS.seek 1ml @19:57:26.15 delay 1 second @19:57:27.17 AFV.select :holdingCoil @19:57:27.27 delay 1 second @19:57:28.30 AS.reconfigure ASfast @19:57:28.35 AS.seek 0ml @19:57:55.72 delay 3 seconds @19:57:58.74 AFV.select :carrierSolution @19:57:58.84 delay 2 seconds @19:58:00.87 AS.reconfigure ASconfig @19:58:00.93 AS.seek 1ml @19:58:58.72 delay 1 second @19:58:59.74 AFV.select :holdingCoil @19:58:59.84 delay 1 second @19:59:00.87 AS.reconfigure ASfast @19:59:00.92 AS.seek 0ml @19:59:28.28 delay 3 seconds @19:59:31.30 AFV.select :carrierSolution @19:59:31.40 delay 2 seconds @19:59:33.43 AS.reconfigure ASconfig @19:59:33.49 AS.seek 1ml @20:00:31.30 delay 1 second @20:00:32.32 AFV.select :holdingCoil @20:00:32.42 delay 1 second @20:00:33.45 AS.reconfigure ASfast @20:00:33.50 AS.seek 0ml @20:01:00.86 delay 3 seconds @20:01:03.88 AFV.select :carrierSolution @20:01:03.98 delay 2 seconds @20:01:06.02 AS.reconfigure ASconfig @20:01:06.07 AS.seek 1ml @20:02:03.86 delay 1 second @20:02:04.88 AFV.select :holdingCoil @20:02:04.98 delay 1 second @20:02:06.01 AS.reconfigure ASfast @20:02:06.06 AS.seek 0ml @20:02:33.42 delay 3 seconds @20:02:36.44 AFV.select :carrierSolution @20:02:36.54 delay 2 seconds @20:02:38.57 AS.reconfigure ASconfig @20:02:38.63 AS.seek 1ml @20:03:36.42 delay 1 second @20:03:37.44 AFV.select :holdingCoil @20:03:37.54 delay 1 second @20:03:38.57 AS.reconfigure ASnorm @20:03:38.62 AS.seek 0ml @20:04:29.35
AFV.select :carrierSolution @20:04:29.46 delay 2 seconds @20:04:31.49 AS.reconfigure ASconfig @20:04:31.54 AS.seek 0.5ml @20:05:00.58 delay 1 second @20:05:01.60 AFV.select :holdingCoil @20:05:01.70 ATV.dial :air1 @20:05:03.20 AS.reconfigure ASslow2 @20:05:03.25 AS.seek 0.506ml @20:05:09.47 ATV.dial :extSrc @20:05:11.17 AS.seek 0.512ml @20:05:17.47 delay 5 seconds @20:05:22.49 ATV.dial :air1 @20:05:24.21 AS.reconfigure ASslow1 @20:05:24.27 AS.seek 0.542ml @20:05:30.62 ATV.dial :waste @20:05:31.19 AS.reconfigure ASconfig @20:05:31.25 AS.seek :empty @20:06:02.92 Setting up PCR for Bb -- writing /var/log/esp/Bb18dec1020h.pcr @20:06:02.94 Building PCR Slug from extSrc with MM3 mix, PP5 primer @20:06:02.97 Priming with carrier @20:06:02.98 ATV.dial ARV @20:06:04.48 ARV.dial :carrier @20:06:04.84 AS.reconfigure ASnorm @20:06:04.90 AS.seek 0.499ml @20:06:30.51 AFV.select :carrierSolution @20:06:30.62 delay 2 seconds @20:06:32.65 AS.reconfigure ASconfig @20:06:32.71 AS.seek 1ml @20:07:01.78 delay 1 second @20:07:02.80 AFV.select :holdingCoil @20:07:02.90 delay 1 second @20:07:03.92 ATV.dial :waste @20:07:05.59 AS.reconfigure ASnorm @20:07:05.66 AS.seek :empty @20:07:56.42 AFV.select :carrierSolution @20:07:56.79 delay 2 seconds @20:07:58.89 AS.reconfigure ASconfig @20:07:58.95 AS.seek 0.5ml @20:08:28.06 delay 1 second @20:08:29.08 AFV.select :holdingCoil @20:08:29.18 Building Slug @20:08:29.19 ATV.dial :ARV @20:08:30.84 delay 2 seconds @20:08:32.86 ARV.dial :air1 @20:08:33.26 delay 2 seconds @20:08:35.33 AS.reconfigure ASslow2 @20:08:35.40 AS.seek 0.506ml @20:08:41.62 delay 2 seconds @20:08:43.64 ARV.dial :MM3 @20:08:44.72 delay 2 seconds @20:08:46.74 Adding MM3 @20:08:46.83 AS.seek 0.524ml @20:09:05.28 delay 25 seconds @20:09:30.30 ARV.dial :PP5 @20:09:30.72 Adding PP5 @20:09:30.73 delay 2 seconds @20:09:32.81 AS.seek 0.53ml @20:09:39.11 delay 10 seconds @20:09:49.14 ARV.dial :air3 @20:09:49.55 adding ARV air @20:09:49.56 delay 2 seconds @20:09:51.64 AS.seek 0.536ml @20:09:57.91 delay 5 seconds @20:10:02.93 ARV.dial :carrier @20:10:04.23 delay 7 seconds @20:10:11.31 AS.seek 0.594ml @20:11:10.00 delay 5 seconds @20:11:15.02 ATV.dial :extSrc @20:11:15.59 delay 2 seconds @20:11:17.66 AS.seek 0.6ml @20:11:24.05 delay 4 seconds @20:11:28.07 ATV.dial ARV @20:11:28.64 delay 2 seconds @20:11:30.66 ARV.dial :carrier @20:11:30.67 delay 2 seconds @20:11:32.75 AS.seek 0.65ml @20:12:23.33 delay 4 seconds @20:12:27.35 ATV.dial PCR @20:12:28.14 ASV.dial :waste1 @20:12:28.15 ARV.dial :air1 @20:12:28.59 Positioning Slug by 0.324ml @20:12:28.68 AS.seek 0.326ml @20:17:54.97 delay 5 seconds @20:17:59.99 ATV.dial ARV @20:18:00.78 ASV.dial :PCRlock @20:18:01.19 delay 5 seconds @20:18:06.21 Running Bb Profile60C300 thermocycling @20:18:06.22 PCR.program Profile60C300 @20:18:06.36 Profile60C300 should complete in < 1:45:21 @20:18:06.37 PCR.run 2:16:41.25 @21:40:09.84 PCR.off @21:40:11.36 PCR for Bb completed -- closed /var/log/esp/Bb18dec1020h.pcr @21:40:11.44 Cleaning PCR @21:40:11.45 AFV.select :carrierSolution @21:40:11.57 delay 2 seconds @21:40:13.60 AS.reconfigure ASconfig @21:40:13.65 AS.seek 0.5ml @21:40:24.00 delay 1 second @21:40:25.02 AFV.select :holdingCoil @21:40:25.12 ASV.dial :waste1 @21:40:25.52 ATV.dial :air3 @21:40:26.81 AS.reconfigure ASslow1 @21:40:26.87 AS.seek 0.51ml @21:40:29.14 delay 2 seconds @21:40:31.16 ATV.dial :bleach @21:40:31.78 AS.seek 0.81ml @21:41:32.38 delay 3 seconds @21:41:35.40 ATV.dial :air3 @21:41:35.96 delay 3 seconds @21:41:39.03 AS.seek 0.835ml @21:41:44.33 delay 3 seconds @21:41:47.35 ATV.dial :bleach @21:41:47.97 AS.seek 0.82ml @21:41:51.28 delay 3 seconds @21:41:54.30 ARV.dial :waste @21:41:54.93 delay 3 seconds @21:41:57.95 ATV.dial :ARV @21:41:58.84 AS.reconfigure ASconfig @21:41:58.91 AS.seek 0.47ml @21:42:19.35 AFV.select :carrierSolution @21:42:19.46 delay 2 seconds @21:42:21.49 AS.seek 1ml @21:42:52.22 delay 1 second @21:42:53.24 AFV.select :holdingCoil @21:42:53.34 delay 1 second @21:42:54.37 AS.reconfigure ASfast @21:42:54.42 AS.seek 0ml @21:43:21.88 AFV.select :carrierSolution @21:43:21.99 delay 2 seconds @21:43:24.02 AS.reconfigure ASconfig @21:43:24.07 AS.seek 1ml @21:44:21.88 delay 1 second @21:44:22.90 AFV.select :holdingCoil @21:44:23.00 delay 1 second @21:44:24.03 AS.reconfigure ASfast @21:44:24.08 AS.seek 0ml @21:44:51.44 AFV.select :carrierSolution @21:44:51.55 delay 2 seconds @21:44:53.58 AS.reconfigure ASconfig @21:44:53.63 AS.seek 1ml @21:45:51.43 delay 1 second @21:45:52.45 AFV.select :holdingCoil @21:45:52.55 delay 1 second @21:45:53.58 AS.reconfigure ASfast @21:45:53.63 AS.seek 0ml @21:46:20.99 ARV.dial between carrier and air1 @21:46:21.50 ATV.dial :air3 @21:46:22.80 AS.reconfigure ASslow1 @21:46:22.86 AS.seek 0.01ml @21:46:25.19 delay 2 seconds @21:46:27.21 ATV.dial :bleach @21:46:27.85 AS.seek 0.81ml @21:49:09.00 delay 3 seconds @21:49:12.02 ATV.dial :air3 @21:49:12.60 delay 3 seconds @21:49:15.67 AS.seek 0.835ml @21:49:20.95 delay 3 seconds @21:49:23.97 ATV.dial :bleach @21:49:24.61 AS.seek 0.82ml @21:49:27.93 delay 3 seconds @21:49:30.95 ATV.dial PCR @21:49:31.35 AS.reconfigure ASconfig @21:49:31.41 AS.seek :empty @21:50:18.88 PCR.program :bleachProfile @21:50:18.94 PCR program should complete in < 2:45 @21:50:18.95 PCR.run 10:00 @21:53:18.13 PCR.off @21:53:18.16 AFV.select :carrierSolution @21:53:18.28 delay 2 seconds @21:53:20.31 AS.seek 1ml @21:54:18.14 delay 1 second @21:54:19.16 AFV.select :holdingCoil @21:54:19.26 delay 1 second @21:54:20.29 AS.reconfigure ASfast @21:54:20.35 AS.seek 0ml @21:54:47.72 delay 3 seconds @21:54:50.74 AFV.select :carrierSolution @21:54:50.84 delay 2 seconds @21:54:52.87 AS.reconfigure ASconfig @21:54:52.93 AS.seek 1ml @21:55:50.74 delay 1 second @21:55:51.76 AFV.select :holdingCoil @21:55:51.86 delay 1 second @21:55:52.89 AS.reconfigure ASfast @21:55:52.94 AS.seek 0ml @21:56:20.47 delay 3 seconds @21:56:23.49 AFV.select :carrierSolution @21:56:23.59 delay 2 seconds @21:56:25.62 AS.reconfigure ASconfig @21:56:25.68 AS.seek 1ml @21:57:23.47 delay 1 second @21:57:24.49 AFV.select :holdingCoil @21:57:24.59 delay 1 second @21:57:25.62 AS.reconfigure ASfast @21:57:25.67 AS.seek 0ml @21:57:53.04 delay 3 seconds @21:57:56.06 AFV.select :carrierSolution @21:57:56.16 delay 2 seconds @21:57:58.19 AS.reconfigure ASconfig @21:57:58.24 AS.seek 1ml @21:58:56.04 delay 1 second @21:58:57.06 AFV.select :holdingCoil @21:58:57.16 delay 1 second @21:58:58.19 AS.reconfigure ASfast @21:58:58.24 AS.seek 0ml @21:59:25.62 delay 3 seconds @21:59:28.64 AFV.select :carrierSolution @21:59:28.74 delay 2 seconds @21:59:30.77 AS.reconfigure ASconfig @21:59:30.83 AS.seek 1ml @22:00:28.65 delay 1 second @22:00:29.67 AFV.select :holdingCoil @22:00:29.77 delay 1 second @22:00:30.80 AS.reconfigure ASnorm @22:00:30.85 AS.seek 0ml @22:01:21.56
AFV.select :carrierSolution @22:01:21.67 delay 2 seconds @22:01:23.70 AS.reconfigure ASconfig @22:01:23.75 AS.seek 0.5ml @22:01:52.83 delay 1 second @22:01:53.85 AFV.select :holdingCoil @22:01:53.95 ATV.dial :air1 @22:01:55.46 AS.reconfigure ASslow1 @22:01:55.52 AS.seek 0.52ml @22:01:59.83 delay 5 seconds @22:02:04.85 ATV.dial :extSrc @22:02:06.66 AS.reconfigure ASslow2 @22:02:06.72 AS.seek 0.545ml @22:02:32.04 delay 10 seconds @22:02:42.06 ATV.dial :air1 @22:02:43.76 AS.reconfigure ASslow1 @22:02:43.82 AS.seek 0.575ml @22:02:50.13 ATV.dial :waste @22:02:50.69 AS.reconfigure ASconfig @22:02:50.75 AS.seek :empty @22:03:24.29 Setting up PCR for Bb -- writing /var/log/esp/Bb18dec1022h.pcr @22:03:24.31 Building PCR Slug from extSrc with MM3 mix, PP5 primer @22:03:24.34 Priming with carrier @22:03:24.36 ATV.dial ARV @22:03:25.86 ARV.dial :carrier @22:03:26.22 AS.reconfigure ASnorm @22:03:26.28 AS.seek 0.499ml @22:03:51.85 AFV.select :carrierSolution @22:03:51.96 delay 2 seconds @22:03:53.99 AS.reconfigure ASconfig @22:03:54.05 AS.seek 1ml @22:04:23.13 delay 1 second @22:04:24.15 AFV.select :holdingCoil @22:04:24.25 delay 1 second @22:04:25.27 ATV.dial :waste @22:04:26.93 AS.reconfigure ASnorm @22:04:27.00 AS.seek :empty @22:05:17.74 AFV.select :carrierSolution @22:05:17.86 delay 2 seconds @22:05:19.89 AS.reconfigure ASconfig @22:05:19.95 AS.seek 0.5ml @22:05:49.07 delay 1 second @22:05:50.09 AFV.select :holdingCoil @22:05:50.19 Building Slug @22:05:50.20 ATV.dial :ARV @22:05:51.85 delay 2 seconds @22:05:53.87 ARV.dial :air1 @22:05:54.29 delay 2 seconds @22:05:56.36 AS.reconfigure ASslow2 @22:05:56.43 AS.seek 0.506ml @22:06:02.71 delay 2 seconds @22:06:04.73 ARV.dial :MM3 @22:06:05.82 delay 2 seconds @22:06:07.84 Adding MM3 @22:06:07.93 AS.seek 0.524ml @22:06:26.46 delay 25 seconds @22:06:51.48 ARV.dial :PP5 @22:06:51.90 Adding PP5 @22:06:51.91 delay 2 seconds @22:06:54.37 AS.seek 0.53ml @22:07:00.72 delay 10 seconds @22:07:10.75 ARV.dial :air3 @22:07:11.13 adding ARV air @22:07:11.14 delay 2 seconds @22:07:13.23 AS.seek 0.536ml @22:07:19.54 delay 5 seconds @22:07:24.56 ARV.dial :carrier @22:07:25.85 delay 7 seconds @22:07:32.93 AS.seek 0.594ml @22:08:31.55 delay 5 seconds @22:08:36.57 ATV.dial :extSrc @22:08:37.13 delay 2 seconds @22:08:39.20 AS.seek 0.6ml @22:08:45.41 delay 4 seconds @22:08:49.43 ATV.dial ARV @22:08:49.99 delay 2 seconds @22:08:52.01 ARV.dial :carrier @22:08:52.02 delay 2 seconds @22:08:54.10 AS.seek 0.65ml @22:09:44.68 delay 4 seconds @22:09:48.70 ATV.dial PCR @22:09:49.47 ASV.dial :waste1 @22:09:49.48 ARV.dial :air1 @22:09:49.91 Positioning Slug by 0.324ml @22:09:50.00 AS.seek 0.326ml @22:15:16.07 delay 5 seconds @22:15:21.09 ATV.dial ARV @22:15:21.88 ASV.dial :PCRlock @22:15:22.29 delay 5 seconds @22:15:27.31 Running Bb Profile60C300 thermocycling @22:15:27.32 PCR.program Profile60C300 @22:15:27.46 Profile60C300 should complete in < 1:45:21 @22:15:27.47 PCR.run 2:16:41.25 @23:37:23.68 PCR.off @23:37:25.17 PCR for Bb completed -- closed /var/log/esp/Bb18dec1022h.pcr @23:37:25.24 Cleaning PCR @23:37:25.25 AFV.select :carrierSolution @23:37:25.38 delay 2 seconds @23:37:27.75 AS.reconfigure ASconfig @23:37:27.86 AS.seek 0.5ml @23:37:38.21 delay 1 second @23:37:39.23 AFV.select :holdingCoil @23:37:39.33 ASV.dial :waste1 @23:37:39.73 ATV.dial :air3 @23:37:41.02 AS.reconfigure ASslow1 @23:37:41.08 AS.seek 0.51ml @23:37:43.36 delay 2 seconds @23:37:45.38 ATV.dial :bleach @23:37:46.01 AS.seek 0.81ml @23:38:46.60 delay 3 seconds @23:38:49.62 ATV.dial :air3 @23:38:50.17 delay 3 seconds @23:38:53.24 AS.seek 0.835ml @23:38:58.55 delay 3 seconds @23:39:01.57 ATV.dial :bleach @23:39:02.20 AS.seek 0.82ml @23:39:05.51 delay 3 seconds @23:39:08.53 ARV.dial :waste @23:39:09.15 delay 3 seconds @23:39:12.17 ATV.dial :ARV @23:39:13.07 AS.reconfigure ASconfig @23:39:13.13 AS.seek 0.47ml @23:39:33.56 AFV.select :carrierSolution @23:39:33.67 delay 2 seconds @23:39:35.70 AS.seek 1ml @23:40:06.46 delay 1 second @23:40:07.48 AFV.select :holdingCoil @23:40:07.58 delay 1 second @23:40:08.61 AS.reconfigure ASfast @23:40:08.66 AS.seek 0ml @23:40:36.03 AFV.select :carrierSolution @23:40:36.14 delay 2 seconds @23:40:38.17 AS.reconfigure ASconfig @23:40:38.22 AS.seek 1ml @23:41:36.03 delay 1 second @23:41:37.05 AFV.select :holdingCoil @23:41:37.15 delay 1 second @23:41:38.18 AS.reconfigure ASfast @23:41:38.23 AS.seek 0ml @23:42:05.60 AFV.select :carrierSolution @23:42:05.71 delay 2 seconds @23:42:07.74 AS.reconfigure ASconfig @23:42:07.79 AS.seek 1ml @23:43:05.56 delay 1 second @23:43:06.58 AFV.select :holdingCoil @23:43:06.68 delay 1 second @23:43:07.71 AS.reconfigure ASfast @23:43:07.76 AS.seek 0ml @23:43:35.12 ARV.dial between carrier and air1 @23:43:35.64 ATV.dial :air3 @23:43:36.93 AS.reconfigure ASslow1 @23:43:36.99 AS.seek 0.01ml @23:43:39.32 delay 2 seconds @23:43:41.34 ATV.dial :bleach @23:43:41.98 AS.seek 0.81ml @23:46:23.17 delay 3 seconds @23:46:26.19 ATV.dial :air3 @23:46:26.76 delay 3 seconds @23:46:29.83 AS.seek 0.835ml @23:46:35.14 delay 3 seconds @23:46:38.16 ATV.dial :bleach @23:46:38.77 AS.seek 0.82ml @23:46:42.07 delay 3 seconds @23:46:45.09 ATV.dial PCR @23:46:45.51 AS.reconfigure ASconfig @23:46:45.57 AS.seek :empty @23:47:33.04 PCR.program :bleachProfile @23:47:33.09 PCR program should complete in < 2:45 @23:47:33.10 PCR.run 10:00 @23:50:31.91 PCR.off @23:50:31.94 AFV.select :carrierSolution @23:50:32.06 delay 2 seconds @23:50:34.09 AS.seek 1ml @23:51:31.93 delay 1 second @23:51:32.95 AFV.select :holdingCoil @23:51:33.05 delay 1 second @23:51:34.08 AS.reconfigure ASfast @23:51:34.13 AS.seek 0ml @23:52:01.59 delay 3 seconds @23:52:04.61 AFV.select :carrierSolution @23:52:04.71 delay 2 seconds @23:52:06.74 AS.reconfigure ASconfig @23:52:06.80 AS.seek 1ml @23:53:04.59 delay 1 second @23:53:05.61 AFV.select :holdingCoil @23:53:05.71 delay 1 second @23:53:06.74 AS.reconfigure ASfast @23:53:06.79 AS.seek 0ml @23:53:34.25 delay 3 seconds @23:53:37.27 AFV.select :carrierSolution @23:53:37.37 delay 2 seconds @23:53:39.40 AS.reconfigure ASconfig @23:53:39.46 AS.seek 1ml @23:54:37.68 delay 1 second @23:54:38.70 AFV.select :holdingCoil @23:54:38.80 delay 1 second @23:54:39.83 AS.reconfigure ASfast @23:54:39.88 AS.seek 0ml @23:55:07.26 delay 3 seconds @23:55:10.28 AFV.select :carrierSolution @23:55:10.38 delay 2 seconds @23:55:12.41 AS.reconfigure ASconfig @23:55:12.47 AS.seek 1ml @23:56:10.28 delay 1 second @23:56:11.30 AFV.select :holdingCoil @23:56:11.40 delay 1 second @23:56:12.43 AS.reconfigure ASfast @23:56:12.48 AS.seek 0ml @23:56:39.84 delay 3 seconds @23:56:42.86 AFV.select :carrierSolution @23:56:42.96 delay 2 seconds @23:56:44.99 AS.reconfigure ASconfig @23:56:45.05 AS.seek 1ml @23:57:42.85 delay 1 second @23:57:43.87 AFV.select :holdingCoil @23:57:43.97 delay 1 second @23:57:45.00 AS.reconfigure ASnorm @23:57:45.05 AS.seek 0ml @23:58:35.80
AFV.select :carrierSolution @23:58:35.91 delay 2 seconds @23:58:37.94 AS.reconfigure ASconfig @23:58:37.99 AS.seek 0.5ml @23:59:07.04 delay 1 second @23:59:08.06 AFV.select :holdingCoil @23:59:08.16 ATV.dial :air1 @23:59:09.67 AS.reconfigure ASslow2 @23:59:09.73 AS.seek 0.506ml @23:59:16.23 ATV.dial :extSrc @23:59:17.93 AS.seek 0.512ml @23:59:24.43 delay 5 seconds @23:59:29.45 ATV.dial :air1 @23:59:31.17 AS.reconfigure ASslow1 @23:59:31.22 AS.seek 0.542ml @23:59:37.56 ATV.dial :waste @23:59:38.12 AS.reconfigure ASconfig @23:59:38.18 AS.seek :empty @00:00:09.82CET11-Dec-18 Setting up PCR for Bb -- writing /var/log/esp/Bb18dec1100h.pcr @00:00:09.84 Building PCR Slug from extSrc with MM3 mix, PP5 primer @00:00:09.87 Priming with carrier @00:00:09.89 ATV.dial ARV @00:00:11.39 ARV.dial :carrier @00:00:11.76 AS.reconfigure ASnorm @00:00:11.82 AS.seek 0.499ml @00:00:37.42 AFV.select :carrierSolution @00:00:37.54 delay 2 seconds @00:00:39.57 AS.reconfigure ASconfig @00:00:39.63 AS.seek 1ml @00:01:08.70 delay 1 second @00:01:09.72 AFV.select :holdingCoil @00:01:09.82 delay 1 second @00:01:10.84 ATV.dial :waste @00:01:12.51 AS.reconfigure ASnorm @00:01:12.58 AS.seek :empty @00:02:03.36 AFV.select :carrierSolution @00:02:03.47 delay 2 seconds @00:02:05.50 AS.reconfigure ASconfig @00:02:05.56 AS.seek 0.5ml @00:02:34.65 delay 1 second @00:02:35.67 AFV.select :holdingCoil @00:02:35.77 Building Slug @00:02:35.78 ATV.dial :ARV @00:02:37.43 delay 2 seconds @00:02:39.45 ARV.dial :air1 @00:02:39.85 delay 2 seconds @00:02:41.92 AS.reconfigure ASslow2 @00:02:41.98 AS.seek 0.506ml @00:02:48.39 delay 2 seconds @00:02:50.41 ARV.dial :MM3 @00:02:51.46 delay 2 seconds @00:02:53.48 Adding MM3 @00:02:53.57 AS.seek 0.524ml @00:03:11.96 delay 25 seconds @00:03:36.98 ARV.dial :PP5 @00:03:37.39 Adding PP5 @00:03:37.40 delay 2 seconds @00:03:39.48 AS.seek 0.53ml @00:03:45.89 delay 10 seconds @00:03:55.92 ARV.dial :air3 @00:03:56.32 adding ARV air @00:03:56.33 delay 2 seconds @00:03:58.41 AS.seek 0.536ml @00:04:04.65 delay 5 seconds @00:04:09.67 ARV.dial :carrier @00:04:10.98 delay 7 seconds @00:04:18.06 AS.seek 0.594ml @00:05:16.57 delay 5 seconds @00:05:21.59 ATV.dial :extSrc @00:05:22.17 delay 2 seconds @00:05:24.24 AS.seek 0.6ml @00:05:30.42 delay 4 seconds @00:05:34.44 ATV.dial ARV @00:05:35.01 delay 2 seconds @00:05:37.03 ARV.dial :carrier @00:05:37.04 delay 2 seconds @00:05:39.12 AS.seek 0.65ml @00:06:29.71 delay 4 seconds @00:06:33.73 ATV.dial PCR @00:06:34.51 ASV.dial :waste1 @00:06:34.52 ARV.dial :air1 @00:06:34.94 Positioning Slug by 0.324ml @00:06:35.03 AS.seek 0.326ml @00:12:00.93 delay 5 seconds @00:12:05.95 ATV.dial ARV @00:12:06.73 ASV.dial :PCRlock @00:12:07.14 delay 5 seconds @00:12:12.16 Running Bb Profile60C300 thermocycling @00:12:12.17 PCR.program Profile60C300 @00:12:12.31 Profile60C300 should complete in < 1:45:21 @00:12:12.32 PCR.run 2:16:41.25 @01:34:12.67 PCR.off @01:34:14.17 PCR for Bb completed -- closed /var/log/esp/Bb18dec1100h.pcr @01:34:14.25 Cleaning PCR @01:34:14.26 AFV.select :carrierSolution @01:34:14.38 delay 2 seconds @01:34:16.41 AS.reconfigure ASconfig @01:34:16.46 AS.seek 0.5ml @01:34:26.77 delay 1 second @01:34:27.79 AFV.select :holdingCoil @01:34:27.90 ASV.dial :waste1 @01:34:28.30 ATV.dial :air3 @01:34:29.58 AS.reconfigure ASslow1 @01:34:29.64 AS.seek 0.51ml @01:34:31.90 delay 2 seconds @01:34:33.92 ATV.dial :bleach @01:34:34.55 AS.seek 0.81ml @01:35:35.16 delay 3 seconds @01:35:38.18 ATV.dial :air3 @01:35:38.74 delay 3 seconds @01:35:41.81 AS.seek 0.835ml @01:35:47.16 delay 3 seconds @01:35:50.18 ATV.dial :bleach @01:35:50.80 AS.seek 0.82ml @01:35:54.11 delay 3 seconds @01:35:57.13 ARV.dial :waste @01:35:58.16 delay 3 seconds @01:36:01.18 ATV.dial :ARV @01:36:02.08 AS.reconfigure ASconfig @01:36:02.14 AS.seek 0.47ml @01:36:22.56 AFV.select :carrierSolution @01:36:22.67 delay 2 seconds @01:36:24.70 AS.seek 1ml @01:36:55.49 delay 1 second @01:36:56.51 AFV.select :holdingCoil @01:36:56.61 delay 1 second @01:36:57.64 AS.reconfigure ASfast @01:36:57.69 AS.seek 0ml @01:37:25.05 AFV.select :carrierSolution @01:37:25.16 delay 2 seconds @01:37:27.19 AS.reconfigure ASconfig @01:37:27.24 AS.seek 1ml @01:38:25.10 delay 1 second @01:38:26.12 AFV.select :holdingCoil @01:38:26.22 delay 1 second @01:38:27.25 AS.reconfigure ASfast @01:38:27.31 AS.seek 0ml @01:38:54.69 AFV.select :carrierSolution @01:38:54.80 delay 2 seconds @01:38:56.83 AS.reconfigure ASconfig @01:38:56.88 AS.seek 1ml @01:39:54.69 delay 1 second @01:39:55.71 AFV.select :holdingCoil @01:39:55.81 delay 1 second @01:39:56.84 AS.reconfigure ASfast @01:39:56.89 AS.seek 0ml @01:40:24.25 ARV.dial between carrier and air1 @01:40:24.77 ATV.dial :air3 @01:40:26.08 AS.reconfigure ASslow1 @01:40:26.14 AS.seek 0.01ml @01:40:28.52 delay 2 seconds @01:40:30.54 ATV.dial :bleach @01:40:31.17 AS.seek 0.81ml @01:43:12.33 delay 3 seconds @01:43:15.35 ATV.dial :air3 @01:43:15.91 delay 3 seconds @01:43:18.98 AS.seek 0.835ml @01:43:24.28 delay 3 seconds @01:43:27.30 ATV.dial :bleach @01:43:27.92 AS.seek 0.82ml @01:43:31.22 delay 3 seconds @01:43:34.24 ATV.dial PCR @01:43:34.66 AS.reconfigure ASconfig @01:43:34.72 AS.seek :empty @01:44:22.20 PCR.program :bleachProfile @01:44:22.25 PCR program should complete in < 2:45 @01:44:22.26 PCR.run 10:00 @01:47:21.08 PCR.off @01:47:21.11 AFV.select :carrierSolution @01:47:21.23 delay 2 seconds @01:47:23.26 AS.seek 1ml @01:48:21.06 delay 1 second @01:48:22.08 AFV.select :holdingCoil @01:48:22.18 delay 1 second @01:48:23.21 AS.reconfigure ASfast @01:48:23.26 AS.seek 0ml @01:48:50.61 delay 3 seconds @01:48:53.63 AFV.select :carrierSolution @01:48:53.73 delay 2 seconds @01:48:55.76 AS.reconfigure ASconfig @01:48:55.82 AS.seek 1ml @01:49:53.63 delay 1 second @01:49:54.65 AFV.select :holdingCoil @01:49:54.75 delay 1 second @01:49:55.78 AS.reconfigure ASfast @01:49:55.83 AS.seek 0ml @01:50:23.21 delay 3 seconds @01:50:26.23 AFV.select :carrierSolution @01:50:26.33 delay 2 seconds @01:50:28.36 AS.reconfigure ASconfig @01:50:28.42 AS.seek 1ml @01:51:26.17 delay 1 second @01:51:27.19 AFV.select :holdingCoil @01:51:27.30 delay 1 second @01:51:28.33 AS.reconfigure ASfast @01:51:28.38 AS.seek 0ml @01:51:55.74 delay 3 seconds @01:51:58.76 AFV.select :carrierSolution @01:51:58.86 delay 2 seconds @01:52:00.89 AS.reconfigure ASconfig @01:52:00.95 AS.seek 1ml @01:52:58.75 delay 1 second @01:52:59.77 AFV.select :holdingCoil @01:52:59.87 delay 1 second @01:53:00.90 AS.reconfigure ASfast @01:53:00.95 AS.seek 0ml @01:53:28.32 delay 3 seconds @01:53:31.34 AFV.select :carrierSolution @01:53:31.44 delay 2 seconds @01:53:33.47 AS.reconfigure ASconfig @01:53:33.53 AS.seek 1ml @01:54:31.35 delay 1 second @01:54:32.37 AFV.select :holdingCoil @01:54:32.47 delay 1 second @01:54:33.50 AS.reconfigure ASnorm @01:54:33.55 AS.seek 0ml @01:55:24.28
AFV.select :carrierSolution @01:55:24.39 delay 2 seconds @01:55:26.42 AS.reconfigure ASconfig @01:55:26.47 AS.seek 0.5ml @01:55:55.53 delay 1 second @01:55:56.55 AFV.select :holdingCoil @01:55:56.65 ATV.dial :air1 @01:55:58.56 AS.reconfigure ASslow1 @01:55:58.61 AS.seek 0.52ml @01:56:02.94 delay 5 seconds @01:56:07.96 ATV.dial :extSrc @01:56:09.67 AS.reconfigure ASslow2 @01:56:09.72 AS.seek 0.538ml @01:56:28.13 delay 10 seconds @01:56:38.16 ATV.dial :air1 @01:56:39.88 AS.reconfigure ASslow1 @01:56:39.93 AS.seek 0.568ml @01:56:46.24 ATV.dial :waste @01:56:46.80 AS.reconfigure ASconfig @01:56:46.86 AS.seek :empty @01:57:19.88 deconEluateRecoveryLine extSrc @01:57:19.89 ATV.dial :waste @01:57:19.92 AS.reconfigure ASnorm @01:57:19.99 AS.seek 0ml @01:57:20.35 ATV.dial :air3 @01:57:21.03 AS.reconfigure ASslow1 @01:57:21.09 AS.seek 0.01ml @01:57:23.38 delay 2 seconds @01:57:25.40 ATV.dial :bleach @01:57:25.88 AS.seek 0.31ml @01:58:26.49 delay 3 seconds @01:58:29.51 ATV.dial :air3 @01:58:30.08 delay 3 seconds @01:58:33.15 AS.seek 0.335ml @01:58:38.47 delay 3 seconds @01:58:41.49 ATV.dial :bleach @01:58:42.11 AS.seek 0.32ml @01:58:45.44 delay 3 seconds @01:58:48.46 ATV.dial :extSrc @01:58:49.09 AS.seek 0ml @01:59:53.72 AFV.select :carrierSolution @01:59:53.83 delay 2 seconds @01:59:55.86 AS.reconfigure ASconfig @01:59:55.91 AS.seek 0.5ml @02:00:24.92 delay 1 second @02:00:25.94 AFV.select :holdingCoil @02:00:26.04 ATV.dial :extSrc @02:00:26.06 AS.reconfigure ASslow1 @02:00:26.13 AS.seek 0ml @02:02:06.96 AFV.select :carrierSolution @02:02:07.07 delay 2 seconds @02:02:09.10 AS.reconfigure ASconfig @02:02:09.15 AS.seek 0.5ml @02:02:38.22 delay 1 second @02:02:39.24 AFV.select :holdingCoil @02:02:39.34 ATV.dial :extSrc @02:02:39.36 AS.reconfigure ASslow1 @02:02:39.43 AS.seek 0ml @02:04:20.28 AFV.select :carrierSolution @02:04:20.39 delay 2 seconds @02:04:22.42 AS.reconfigure ASconfig @02:04:22.47 AS.seek 0.5ml @02:04:51.52 delay 1 second @02:04:52.54 AFV.select :holdingCoil @02:04:52.64 ATV.dial :extSrc @02:04:52.66 AS.reconfigure ASslow1 @02:04:52.73 AS.seek 0ml @02:06:33.57 AFV.select :carrierSolution @02:06:33.68 delay 2 seconds @02:06:35.71 AS.reconfigure ASconfig @02:06:35.76 AS.seek 0.5ml @02:07:04.78 delay 1 second @02:07:05.80 AFV.select :holdingCoil @02:07:05.90 ATV.dial :extSrc @02:07:05.92 AS.reconfigure ASslow1 @02:07:05.99 AS.seek 0ml @02:08:46.83 ATV.dial :air2 @02:08:48.15 AS.reconfigure ASslow0 @02:08:48.21 AS.seek 0.5ml @02:09:38.83 ATV.dial :extSrc @02:09:40.31 AS.seek 0.02ml @02:10:28.93 delay 1 second @02:10:29.95 ATV.dial :waste @02:10:31.24 AS.reconfigure ASconfig @02:10:31.30 AS.seek :empty @02:10:32.82 deconEluateRecoveryLine extSrc @02:10:32.83 ATV.dial :waste @02:10:32.86 AS.reconfigure ASnorm @02:10:32.93 AS.seek 0ml @02:10:33.28 ATV.dial :air3 @02:10:34.14 AS.reconfigure ASslow1 @02:10:34.20 AS.seek 0.01ml @02:10:36.47 delay 2 seconds @02:10:38.49 ATV.dial :bleach @02:10:38.95 AS.seek 0.31ml @02:11:39.55 delay 3 seconds @02:11:42.57 ATV.dial :air3 @02:11:43.13 delay 3 seconds @02:11:46.20 AS.seek 0.335ml @02:11:51.51 delay 3 seconds @02:11:54.53 ATV.dial :bleach @02:11:55.16 AS.seek 0.32ml @02:11:58.47 delay 3 seconds @02:12:01.49 ATV.dial :extSrc @02:12:02.12 AS.seek 0ml @02:13:06.74 AFV.select :carrierSolution @02:13:06.85 delay 2 seconds @02:13:08.88 AS.reconfigure ASconfig @02:13:08.93 AS.seek 0.5ml @02:13:37.97 delay 1 second @02:13:38.99 AFV.select :holdingCoil @02:13:39.09 ATV.dial :extSrc @02:13:39.11 AS.reconfigure ASslow1 @02:13:39.54 AS.seek 0ml @02:15:20.38 AFV.select :carrierSolution @02:15:20.49 delay 2 seconds @02:15:22.52 AS.reconfigure ASconfig @02:15:22.57 AS.seek 0.5ml @02:15:51.61 delay 1 second @02:15:52.63 AFV.select :holdingCoil @02:15:52.73 ATV.dial :extSrc @02:15:52.75 AS.reconfigure ASslow1 @02:15:52.82 AS.seek 0ml @02:17:33.64 AFV.select :carrierSolution @02:17:33.75 delay 2 seconds @02:17:35.78 AS.reconfigure ASconfig @02:17:35.83 AS.seek 0.5ml @02:18:04.89 delay 1 second @02:18:05.91 AFV.select :holdingCoil @02:18:06.01 ATV.dial :extSrc @02:18:06.03 AS.reconfigure ASslow1 @02:18:06.10 AS.seek 0ml @02:19:46.96 AFV.select :carrierSolution @02:19:47.07 delay 2 seconds @02:19:49.10 AS.reconfigure ASconfig @02:19:49.15 AS.seek 0.5ml @02:20:18.19 delay 1 second @02:20:19.21 AFV.select :holdingCoil @02:20:19.31 ATV.dial :extSrc @02:20:19.33 AS.reconfigure ASslow1 @02:20:19.40 AS.seek 0ml @02:22:00.86 ATV.dial :air2 @02:22:02.19 AS.reconfigure ASslow0 @02:22:02.25 AS.seek 0.5ml @02:22:52.88 ATV.dial :extSrc @02:22:54.36 AS.seek 0.02ml @02:23:42.99 delay 1 second @02:23:44.01 ATV.dial :waste @02:23:45.30 AS.reconfigure ASconfig @02:23:45.36 AS.seek :empty @02:23:46.85 deconEluateRecoveryLine extSrc @02:23:46.86 ATV.dial :waste @02:23:46.89 AS.reconfigure ASnorm @02:23:46.96 AS.seek 0ml @02:23:47.35 ATV.dial :air3 @02:23:48.20 AS.reconfigure ASslow1 @02:23:48.25 AS.seek 0.01ml @02:23:50.54 delay 2 seconds @02:23:52.56 ATV.dial :bleach @02:23:53.02 AS.seek 0.31ml @02:24:53.63 delay 3 seconds @02:24:56.65 ATV.dial :air3 @02:24:57.21 delay 3 seconds @02:25:00.28 AS.seek 0.335ml @02:25:05.67 delay 3 seconds @02:25:08.69 ATV.dial :bleach @02:25:09.31 AS.seek 0.32ml @02:25:12.61 delay 3 seconds @02:25:15.63 ATV.dial :extSrc @02:25:16.26 AS.seek 0ml @02:26:20.88 AFV.select :carrierSolution @02:26:20.99 delay 2 seconds @02:26:23.02 AS.reconfigure ASconfig @02:26:23.07 AS.seek 0.5ml @02:26:52.11 delay 1 second @02:26:53.13 AFV.select :holdingCoil @02:26:53.23 ATV.dial :extSrc @02:26:53.25 AS.reconfigure ASslow1 @02:26:53.32 AS.seek 0ml @02:28:34.16 AFV.select :carrierSolution @02:28:34.27 delay 2 seconds @02:28:36.30 AS.reconfigure ASconfig @02:28:36.35 AS.seek 0.5ml @02:29:05.40 delay 1 second @02:29:06.42 AFV.select :holdingCoil @02:29:06.52 ATV.dial :extSrc @02:29:06.54 AS.reconfigure ASslow1 @02:29:06.61 AS.seek 0ml @02:30:47.44 AFV.select :carrierSolution @02:30:47.55 delay 2 seconds @02:30:49.58 AS.reconfigure ASconfig @02:30:49.63 AS.seek 0.5ml @02:31:18.68 delay 1 second @02:31:19.70 AFV.select :holdingCoil @02:31:19.80 ATV.dial :extSrc @02:31:19.82 AS.reconfigure ASslow1 @02:31:19.89 AS.seek 0ml @02:33:00.75 AFV.select :carrierSolution @02:33:00.86 delay 2 seconds @02:33:02.89 AS.reconfigure ASconfig @02:33:02.94 AS.seek 0.5ml @02:33:31.99 delay 1 second @02:33:33.01 AFV.select :holdingCoil @02:33:33.11 ATV.dial :extSrc @02:33:33.13 AS.reconfigure ASslow1 @02:33:33.20 AS.seek 0ml @02:35:14.07 ATV.dial :air2 @02:35:15.38 AS.reconfigure ASslow0 @02:35:15.44 AS.seek 0.5ml @02:36:06.00 ATV.dial :extSrc @02:36:07.58 AS.seek 0.02ml @02:36:56.18 delay 1 second @02:36:57.20 ATV.dial :waste @02:36:58.51 AS.reconfigure ASconfig @02:36:58.57 AS.seek :empty @02:37:00.13 Session Ended @15:49:21.53CET11-Dec-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @15:49:23.21 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @15:49:23.47 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @15:49:27.81 Polling Can every 10:00 @15:49:29.56 SPE.coast @15:49:29.60 SPE.configure SPEconfig @15:49:29.69 AS.reconfigure ASconfig @15:49:29.72 AS.hold @15:49:29.78 AS.configure ASconfig @15:49:29.87 SS.reconfigure SSconfig @15:49:29.91 SS.hold @15:49:29.95 SS.configure SSconfig @15:49:30.04 CC.reconfigure CCconfig @15:49:30.08 CC.hold @15:49:30.15 CC.configure CCconfig @15:49:30.24 CS.reconfigure CSconfig @15:49:30.27 CS.hold @15:49:30.33 CS.configure CSconfig @15:49:30.75 PC.reconfigure PCconfig @15:49:30.79 PC.hold @15:49:30.84 PC.configure PCconfig @15:49:30.93 PS.configure PSconfig @15:49:31.02 Forearm.reconfigure LooseForearm @15:49:31.06 Forearm.hold @15:49:31.11 Forearm.configure LooseForearm @15:49:31.19 Elbow.reconfigure LooseElbow @15:49:31.23 Elbow.hold @15:49:31.29 Elbow.configure LooseElbow @15:49:31.38 SC.reconfigure SCloose @15:49:31.42 SC.hold @15:49:31.48 SC.configure SCloose @15:49:31.56 SE.reconfigure SEconfig @15:49:31.59 SE.hold @15:49:31.65 SE.configure SEconfig @15:49:31.68 CH.coast @15:49:31.73 CH.configure CHconfig @15:49:31.76 PH.coast @15:49:31.81 PH.configure PHconfig @15:49:31.84 PCR.off @15:49:31.86 PCR.pidtemp= 50,0.1,0,300,25,25 @15:49:31.89 PCR.caltemp= 2.7073,0.0373,0 @15:49:31.92 All dwarf controllers running firmware version 3.75 @15:49:32.04 Cleanly exited at 15:47:41CET11-Dec-18 @15:49:32.07 Loading /home/esp/esp2/mission/stdMER2017_MWJ.rb mission @15:49:32.60 AFV.select :carrierSolution @15:49:32.69 delay 2 seconds @15:49:34.72 AS.seek 0.5ml @15:49:39.36 Session Begun @15:50:03.79
delay 1 second @15:50:04.81 AFV.select :holdingCoil @15:50:04.91 ATV.dial :extSrc @15:50:06.40 AS.reconfigure ASslow1 @15:50:06.46 AS.seek 0.53ml @15:50:12.74 delay 5 seconds @15:50:17.82 AS.reconfigure ASslow2 @15:50:17.88 AS.seek 0.54ml @15:50:28.19 delay 20 seconds @15:50:48.27 AS.reconfigure ASslow1 @15:50:48.33 AS.seek 0.57ml @15:50:54.66 delay 5 seconds @15:50:59.74 AS.reconfigure ASslow2 @15:50:59.80 AS.seek 0.58ml @15:51:10.10 delay 20 seconds @15:51:30.17 AS.reconfigure ASslow1 @15:51:30.23 AS.seek 0.61ml @15:51:36.57 delay 5 seconds @15:51:41.68 AS.reconfigure ASslow2 @15:51:41.74 AS.seek 0.62ml @15:51:52.15 delay 20 seconds @15:52:12.23 AS.reconfigure ASslow1 @15:52:12.29 AS.seek 0.65ml @15:52:18.59 delay 5 seconds @15:52:23.66 AS.reconfigure ASslow2 @15:52:23.72 AS.seek 0.66ml @15:52:33.91 delay 20 seconds @15:52:53.95 AS.reconfigure ASslow1 @15:52:54.00 AS.seek 0.705ml @15:53:03.34 delay 10 seconds @15:53:13.37 ATV.dial :air1 @15:53:15.10 AS.seek 0.725ml @15:53:19.37 delay 3 seconds @15:53:22.39 ATV.dial :waste @15:53:22.94 AS.reconfigure ASslow0 @15:53:23.00 AS.seek :empty @15:54:36.43 Setting up PCR for Rp -- writing /var/log/esp/Rp18dec1115h.pcr @15:54:36.45 Building PCR Slug from extSrc with MM2 mix, PP3 primer @15:54:36.48 Priming with carrier @15:54:36.49 ATV.dial ARV @15:54:38.01 ARV.dial :carrier @15:54:38.36 AS.reconfigure ASnorm @15:54:38.42 AS.seek 0.499ml @15:55:03.98 AFV.select :carrierSolution @15:55:04.10 delay 2 seconds @15:55:06.13 AS.reconfigure ASconfig @15:55:06.19 AS.seek 1ml @15:55:35.26 delay 1 second @15:55:36.28 AFV.select :holdingCoil @15:55:36.38 delay 1 second @15:55:37.40 ATV.dial :waste @15:55:39.09 AS.reconfigure ASnorm @15:55:39.15 AS.seek :empty @15:56:29.91 AFV.select :carrierSolution @15:56:30.02 delay 2 seconds @15:56:32.05 AS.reconfigure ASconfig @15:56:32.10 AS.seek 0.5ml @15:57:01.21 delay 1 second @15:57:02.23 AFV.select :holdingCoil @15:57:02.33 Building Slug @15:57:02.34 ATV.dial :ARV @15:57:04.01 delay 2 seconds @15:57:06.03 ARV.dial :air1 @15:57:06.45 delay 2 seconds @15:57:08.52 AS.reconfigure ASslow2 @15:57:08.58 AS.seek 0.506ml @15:57:14.77 delay 2 seconds @15:57:16.79 ARV.dial :MM2 @15:57:18.32 delay 2 seconds @15:57:20.34 Adding MM2 @15:57:20.42 AS.seek 0.524ml @15:57:38.77 delay 25 seconds @15:58:03.79 ARV.dial :PP3 @15:58:04.20 Adding PP3 @15:58:04.21 delay 2 seconds @15:58:06.29 AS.seek 0.53ml @15:58:12.82 delay 10 seconds @15:58:22.85 ARV.dial :air2 @15:58:23.24 adding ARV air @15:58:23.25 delay 2 seconds @15:58:25.33 AS.seek 0.536ml @15:58:31.65 delay 5 seconds @15:58:36.67 ARV.dial :carrier @15:58:37.99 delay 7 seconds @15:58:45.07 AS.seek 0.594ml @15:59:43.55 delay 5 seconds @15:59:48.57 ATV.dial :extSrc @15:59:49.13 delay 2 seconds @15:59:51.20 AS.seek 0.6ml @15:59:57.40 delay 4 seconds @16:00:01.42 ATV.dial ARV @16:00:01.98 delay 2 seconds @16:00:04.00 ARV.dial :carrier @16:00:04.01 delay 2 seconds @16:00:06.09 AS.seek 0.65ml @16:00:56.55 delay 4 seconds @16:01:00.57 ATV.dial PCR @16:01:01.35 ASV.dial :waste1 @16:01:01.45 ARV.dial :air1 @16:01:02.27 Positioning Slug by 0.324ml @16:01:02.36 AS.seek 0.326ml @16:06:28.49 delay 5 seconds @16:06:33.51 ATV.dial ARV @16:06:34.29 ASV.dial :PCRlock @16:06:34.70 delay 5 seconds @16:06:39.72 Running Rp Profile60C300 thermocycling @16:06:39.74 PCR.program Profile60C300 @16:06:39.88 Profile60C300 should complete in < 1:45:21 @16:06:39.89 PCR.run 2:16:41.25 @17:29:03.59 PCR.off @17:29:05.10 PCR for Rp completed -- closed /var/log/esp/Rp18dec1115h.pcr @17:29:05.17 Cleaning PCR @17:29:05.18 AFV.select :carrierSolution @17:29:05.30 delay 2 seconds @17:29:07.33 AS.reconfigure ASconfig @17:29:07.38 AS.seek 0.5ml @17:29:17.70 delay 1 second @17:29:18.72 AFV.select :holdingCoil @17:29:18.83 ASV.dial :waste1 @17:29:19.23 ATV.dial :air3 @17:29:20.52 AS.reconfigure ASslow1 @17:29:20.58 AS.seek 0.51ml @17:29:22.87 delay 2 seconds @17:29:24.89 ATV.dial :bleach @17:29:25.51 AS.seek 0.81ml @17:30:26.06 delay 3 seconds @17:30:29.08 ATV.dial :air3 @17:30:29.64 delay 3 seconds @17:30:32.71 AS.seek 0.835ml @17:30:38.04 delay 3 seconds @17:30:41.06 ATV.dial :bleach @17:30:41.68 AS.seek 0.82ml @17:30:44.98 delay 3 seconds @17:30:48.00 ARV.dial :waste @17:30:48.62 delay 3 seconds @17:30:51.64 ATV.dial :ARV @17:30:52.53 AS.reconfigure ASconfig @17:30:52.60 AS.seek 0.47ml @17:31:13.02 AFV.select :carrierSolution @17:31:13.14 delay 2 seconds @17:31:15.17 AS.seek 1ml @17:31:45.90 delay 1 second @17:31:46.92 AFV.select :holdingCoil @17:31:47.03 delay 1 second @17:31:48.06 AS.reconfigure ASfast @17:31:48.11 AS.seek 0ml @17:32:15.47 AFV.select :carrierSolution @17:32:15.58 delay 2 seconds @17:32:17.60 AS.reconfigure ASconfig @17:32:17.66 AS.seek 1ml @17:33:15.45 delay 1 second @17:33:16.47 AFV.select :holdingCoil @17:33:16.58 delay 1 second @17:33:17.94 AS.reconfigure ASfast @17:33:18.06 AS.seek 0ml @17:33:45.43 AFV.select :carrierSolution @17:33:45.54 delay 2 seconds @17:33:47.56 AS.reconfigure ASconfig @17:33:47.62 AS.seek 1ml @17:34:45.37 delay 1 second @17:34:46.39 AFV.select :holdingCoil @17:34:46.49 delay 1 second @17:34:47.52 AS.reconfigure ASfast @17:34:47.57 AS.seek 0ml @17:35:15.36 ARV.dial between carrier and air1 @17:35:15.89 ATV.dial :air3 @17:35:17.20 AS.reconfigure ASslow1 @17:35:17.26 AS.seek 0.01ml @17:35:19.56 delay 2 seconds @17:35:21.58 ATV.dial :bleach @17:35:22.21 AS.seek 0.81ml @17:38:03.37 delay 3 seconds @17:38:06.39 ATV.dial :air3 @17:38:06.97 delay 3 seconds @17:38:10.04 AS.seek 0.835ml @17:38:15.33 delay 3 seconds @17:38:18.35 ATV.dial :bleach @17:38:18.98 AS.seek 0.82ml @17:38:22.29 delay 3 seconds @17:38:25.31 ATV.dial PCR @17:38:25.72 AS.reconfigure ASconfig @17:38:25.78 AS.seek :empty @17:39:13.25 PCR.program :bleachProfile @17:39:13.30 PCR program should complete in < 2:45 @17:39:13.32 PCR.run 10:00 @17:42:09.10 PCR.off @17:42:09.13 AFV.select :carrierSolution @17:42:09.25 delay 2 seconds @17:42:11.28 AS.seek 1ml @17:43:09.09 delay 1 second @17:43:10.11 AFV.select :holdingCoil @17:43:10.22 delay 1 second @17:43:11.25 AS.reconfigure ASfast @17:43:11.30 AS.seek 0ml @17:43:38.65 delay 3 seconds @17:43:41.67 AFV.select :carrierSolution @17:43:41.77 delay 2 seconds @17:43:43.79 AS.reconfigure ASconfig @17:43:43.86 AS.seek 1ml @17:44:41.65 delay 1 second @17:44:42.67 AFV.select :holdingCoil @17:44:42.77 delay 1 second @17:44:43.80 AS.reconfigure ASfast @17:44:43.85 AS.seek 0ml @17:45:11.22 delay 3 seconds @17:45:14.24 AFV.select :carrierSolution @17:45:14.34 delay 2 seconds @17:45:16.36 AS.reconfigure ASconfig @17:45:16.42 AS.seek 1ml @17:46:14.22 delay 1 second @17:46:15.24 AFV.select :holdingCoil @17:46:15.34 delay 1 second @17:46:16.37 AS.reconfigure ASfast @17:46:16.42 AS.seek 0ml @17:46:43.79 delay 3 seconds @17:46:46.81 AFV.select :carrierSolution @17:46:46.91 delay 2 seconds @17:46:48.93 AS.reconfigure ASconfig @17:46:49.00 AS.seek 1ml @17:47:46.76 delay 1 second @17:47:47.78 AFV.select :holdingCoil @17:47:47.89 delay 1 second @17:47:48.92 AS.reconfigure ASfast @17:47:48.97 AS.seek 0ml @17:48:16.33 delay 3 seconds @17:48:19.35 AFV.select :carrierSolution @17:48:19.45 delay 2 seconds @17:48:21.47 AS.reconfigure ASconfig @17:48:21.53 AS.seek 1ml @17:49:19.37 delay 1 second @17:49:20.39 AFV.select :holdingCoil @17:49:20.50 delay 1 second @17:49:21.52 AS.reconfigure ASnorm @17:49:21.58 AS.seek 0ml @17:50:12.29
AFV.select :carrierSolution @17:50:12.40 delay 2 seconds @17:50:14.42 AS.reconfigure ASconfig @17:50:14.48 AS.seek 0.5ml @17:50:43.56 delay 1 second @17:50:44.58 AFV.select :holdingCoil @17:50:44.68 ATV.dial :air1 @17:50:46.18 AS.reconfigure ASslow2 @17:50:46.24 AS.seek 0.506ml @17:50:52.50 ATV.dial :extSrc @17:50:54.21 AS.seek 0.512ml @17:51:00.68 delay 5 seconds @17:51:05.70 ATV.dial :air1 @17:51:07.42 AS.reconfigure ASslow1 @17:51:07.48 AS.seek 0.542ml @17:51:13.83 ATV.dial :waste @17:51:14.39 AS.reconfigure ASconfig @17:51:14.45 AS.seek :empty @17:51:46.12 Setting up PCR for Rp -- writing /var/log/esp/Rp18dec1117h.pcr @17:51:46.14 Building PCR Slug from extSrc with MM2 mix, PP3 primer @17:51:46.17 Priming with carrier @17:51:46.19 ATV.dial ARV @17:51:47.68 ARV.dial :carrier @17:51:48.04 AS.reconfigure ASnorm @17:51:48.10 AS.seek 0.499ml @17:52:13.75 AFV.select :carrierSolution @17:52:13.86 delay 2 seconds @17:52:15.89 AS.reconfigure ASconfig @17:52:15.94 AS.seek 1ml @17:52:45.00 delay 1 second @17:52:46.02 AFV.select :holdingCoil @17:52:46.12 delay 1 second @17:52:47.14 ATV.dial :waste @17:52:48.81 AS.reconfigure ASnorm @17:52:48.87 AS.seek :empty @17:53:39.62 AFV.select :carrierSolution @17:53:39.73 delay 2 seconds @17:53:41.76 AS.reconfigure ASconfig @17:53:41.81 AS.seek 0.5ml @17:54:10.93 delay 1 second @17:54:11.95 AFV.select :holdingCoil @17:54:12.05 Building Slug @17:54:12.06 ATV.dial :ARV @17:54:13.81 delay 2 seconds @17:54:15.83 ARV.dial :air1 @17:54:16.24 delay 2 seconds @17:54:18.31 AS.reconfigure ASslow2 @17:54:18.37 AS.seek 0.506ml @17:54:24.58 delay 2 seconds @17:54:26.60 ARV.dial :MM2 @17:54:28.12 delay 2 seconds @17:54:30.14 Adding MM2 @17:54:30.23 AS.seek 0.524ml @17:54:49.12 delay 25 seconds @17:55:14.14 ARV.dial :PP3 @17:55:14.54 Adding PP3 @17:55:14.55 delay 2 seconds @17:55:16.63 AS.seek 0.53ml @17:55:22.97 delay 10 seconds @17:55:33.00 ARV.dial :air2 @17:55:33.40 adding ARV air @17:55:33.41 delay 2 seconds @17:55:35.49 AS.seek 0.536ml @17:55:41.72 delay 5 seconds @17:55:46.74 ARV.dial :carrier @17:55:48.06 delay 7 seconds @17:55:55.14 AS.seek 0.594ml @17:56:53.72 delay 5 seconds @17:56:58.74 ATV.dial :extSrc @17:56:59.29 delay 2 seconds @17:57:01.36 AS.seek 0.6ml @17:57:07.79 delay 4 seconds @17:57:11.81 ATV.dial ARV @17:57:12.37 delay 2 seconds @17:57:14.39 ARV.dial :carrier @17:57:14.40 delay 2 seconds @17:57:16.48 AS.seek 0.65ml @17:58:07.11 delay 4 seconds @17:58:11.13 ATV.dial PCR @17:58:11.90 ASV.dial :waste1 @17:58:11.91 ARV.dial :air1 @17:58:12.35 Positioning Slug by 0.324ml @17:58:12.44 AS.seek 0.326ml @18:03:38.50 delay 5 seconds @18:03:43.52 ATV.dial ARV @18:03:44.31 ASV.dial :PCRlock @18:03:44.72 delay 5 seconds @18:03:49.74 Running Rp Profile60C300 thermocycling @18:03:49.75 PCR.program Profile60C300 @18:03:49.89 Profile60C300 should complete in < 1:45:21 @18:03:49.90 PCR.run 2:16:41.25 @19:26:12.78 PCR.off @19:26:14.28 PCR for Rp completed -- closed /var/log/esp/Rp18dec1117h.pcr @19:26:14.36 Cleaning PCR @19:26:14.37 AFV.select :carrierSolution @19:26:14.49 delay 2 seconds @19:26:16.51 AS.reconfigure ASconfig @19:26:16.57 AS.seek 0.5ml @19:26:26.88 delay 1 second @19:26:27.90 AFV.select :holdingCoil @19:26:28.01 ASV.dial :waste1 @19:26:28.41 ATV.dial :air3 @19:26:30.12 AS.reconfigure ASslow1 @19:26:30.18 AS.seek 0.51ml @19:26:32.47 delay 2 seconds @19:26:34.49 ATV.dial :bleach @19:26:35.12 AS.seek 0.81ml @19:27:35.70 delay 3 seconds @19:27:38.72 ATV.dial :air3 @19:27:39.28 delay 3 seconds @19:27:42.35 AS.seek 0.835ml @19:27:47.66 delay 3 seconds @19:27:50.68 ATV.dial :bleach @19:27:51.31 AS.seek 0.82ml @19:27:54.61 delay 3 seconds @19:27:57.63 ARV.dial :waste @19:27:58.25 delay 3 seconds @19:28:01.27 ATV.dial :ARV @19:28:02.16 AS.reconfigure ASconfig @19:28:02.22 AS.seek 0.47ml @19:28:22.63 AFV.select :carrierSolution @19:28:22.74 delay 2 seconds @19:28:24.77 AS.seek 1ml @19:28:55.56 delay 1 second @19:28:56.58 AFV.select :holdingCoil @19:28:56.68 delay 1 second @19:28:57.71 AS.reconfigure ASfast @19:28:57.76 AS.seek 0ml @19:29:25.13 AFV.select :carrierSolution @19:29:25.24 delay 2 seconds @19:29:27.26 AS.reconfigure ASconfig @19:29:27.32 AS.seek 1ml @19:30:25.13 delay 1 second @19:30:26.15 AFV.select :holdingCoil @19:30:26.26 delay 1 second @19:30:27.29 AS.reconfigure ASfast @19:30:27.34 AS.seek 0ml @19:30:54.72 AFV.select :carrierSolution @19:30:54.83 delay 2 seconds @19:30:56.85 AS.reconfigure ASconfig @19:30:56.91 AS.seek 1ml @19:31:54.69 delay 1 second @19:31:55.71 AFV.select :holdingCoil @19:31:55.81 delay 1 second @19:31:56.84 AS.reconfigure ASfast @19:31:56.89 AS.seek 0ml @19:32:24.24 ARV.dial between carrier and air1 @19:32:24.77 ATV.dial :air3 @19:32:26.06 AS.reconfigure ASslow1 @19:32:26.12 AS.seek 0.01ml @19:32:28.49 delay 2 seconds @19:32:30.51 ATV.dial :bleach @19:32:31.14 AS.seek 0.81ml @19:35:12.31 delay 3 seconds @19:35:15.33 ATV.dial :air3 @19:35:15.89 delay 3 seconds @19:35:18.96 AS.seek 0.835ml @19:35:24.28 delay 3 seconds @19:35:27.30 ATV.dial :bleach @19:35:27.94 AS.seek 0.82ml @19:35:31.25 delay 3 seconds @19:35:34.27 ATV.dial PCR @19:35:34.67 AS.reconfigure ASconfig @19:35:34.73 AS.seek :empty @19:36:22.22 PCR.program :bleachProfile @19:36:22.28 PCR program should complete in < 2:45 @19:36:22.29 PCR.run 10:00 @19:39:18.06 PCR.off @19:39:18.09 AFV.select :carrierSolution @19:39:18.20 delay 2 seconds @19:39:20.23 AS.seek 1ml @19:40:18.06 delay 1 second @19:40:19.08 AFV.select :holdingCoil @19:40:19.19 delay 1 second @19:40:20.22 AS.reconfigure ASfast @19:40:20.27 AS.seek 0ml @19:40:47.64 delay 3 seconds @19:40:50.66 AFV.select :carrierSolution @19:40:50.76 delay 2 seconds @19:40:52.78 AS.reconfigure ASconfig @19:40:52.85 AS.seek 1ml @19:41:50.70 delay 1 second @19:41:51.72 AFV.select :holdingCoil @19:41:51.83 delay 1 second @19:41:52.85 AS.reconfigure ASfast @19:41:52.91 AS.seek 0ml @19:42:20.28 delay 3 seconds @19:42:23.30 AFV.select :carrierSolution @19:42:23.40 delay 2 seconds @19:42:25.42 AS.reconfigure ASconfig @19:42:25.48 AS.seek 1ml @19:43:23.27 delay 1 second @19:43:24.29 AFV.select :holdingCoil @19:43:24.39 delay 1 second @19:43:25.41 AS.reconfigure ASfast @19:43:25.47 AS.seek 0ml @19:43:52.84 delay 3 seconds @19:43:55.86 AFV.select :carrierSolution @19:43:55.97 delay 2 seconds @19:43:57.99 AS.reconfigure ASconfig @19:43:58.06 AS.seek 1ml @19:44:55.86 delay 1 second @19:44:56.88 AFV.select :holdingCoil @19:44:56.99 delay 1 second @19:44:58.01 AS.reconfigure ASfast @19:44:58.07 AS.seek 0ml @19:45:25.42 delay 3 seconds @19:45:28.44 AFV.select :carrierSolution @19:45:28.54 delay 2 seconds @19:45:30.56 AS.reconfigure ASconfig @19:45:30.63 AS.seek 1ml @19:46:28.42 delay 1 second @19:46:29.44 AFV.select :holdingCoil @19:46:29.55 delay 1 second @19:46:30.57 AS.reconfigure ASnorm @19:46:30.63 AS.seek 0ml @19:47:21.37
AFV.select :carrierSolution @19:47:21.48 delay 2 seconds @19:47:23.50 AS.reconfigure ASconfig @19:47:23.56 AS.seek 0.5ml @19:47:52.59 delay 1 second @19:47:53.61 AFV.select :holdingCoil @19:47:53.71 ATV.dial :air1 @19:47:55.22 AS.reconfigure ASslow2 @19:47:55.27 AS.seek 0.51ml @19:48:05.67 delay 5 seconds @19:48:10.69 ATV.dial :extSrc @19:48:12.42 AS.seek 0.53ml @19:48:32.95 delay 10 seconds @19:48:42.98 ATV.dial :air1 @19:48:44.71 AS.reconfigure ASslow1 @19:48:44.77 AS.seek 0.56ml @19:48:51.08 ATV.dial :waste @19:48:52.04 AS.reconfigure ASconfig @19:48:52.10 AS.seek :empty @19:49:24.79 Setting up PCR for Rp -- writing /var/log/esp/Rp18dec1119h.pcr @19:49:24.81 Building PCR Slug from extSrc with MM2 mix, PP3 primer @19:49:24.84 Priming with carrier @19:49:24.86 ATV.dial ARV @19:49:26.47 ARV.dial :carrier @19:49:26.83 AS.reconfigure ASnorm @19:49:26.89 AS.seek 0.499ml @19:49:52.47 AFV.select :carrierSolution @19:49:52.59 delay 2 seconds @19:49:54.62 AS.reconfigure ASconfig @19:49:54.68 AS.seek 1ml @19:50:23.75 delay 1 second @19:50:24.77 AFV.select :holdingCoil @19:50:24.87 delay 1 second @19:50:25.89 ATV.dial :waste @19:50:27.56 AS.reconfigure ASnorm @19:50:27.62 AS.seek :empty @19:51:18.41 AFV.select :carrierSolution @19:51:18.52 delay 2 seconds @19:51:20.55 AS.reconfigure ASconfig @19:51:20.61 AS.seek 0.5ml @19:51:49.74 delay 1 second @19:51:50.76 AFV.select :holdingCoil @19:51:50.86 Building Slug @19:51:50.87 ATV.dial :ARV @19:51:52.64 delay 2 seconds @19:51:54.66 ARV.dial :air1 @19:51:55.07 delay 2 seconds @19:51:57.14 AS.reconfigure ASslow2 @19:51:57.20 AS.seek 0.506ml @19:52:03.41 delay 2 seconds @19:52:05.43 ARV.dial :MM2 @19:52:06.95 delay 2 seconds @19:52:08.97 Adding MM2 @19:52:09.06 AS.seek 0.524ml @19:52:27.39 delay 25 seconds @19:52:52.41 ARV.dial :PP3 @19:52:52.81 Adding PP3 @19:52:52.82 delay 2 seconds @19:52:54.90 AS.seek 0.53ml @19:53:01.33 delay 10 seconds @19:53:11.36 ARV.dial :air2 @19:53:11.77 adding ARV air @19:53:11.78 delay 2 seconds @19:53:13.86 AS.seek 0.536ml @19:53:20.10 delay 5 seconds @19:53:25.12 ARV.dial :carrier @19:53:26.44 delay 7 seconds @19:53:33.52 AS.seek 0.594ml @19:54:32.03 delay 5 seconds @19:54:37.05 ATV.dial :extSrc @19:54:37.63 delay 2 seconds @19:54:39.70 AS.seek 0.6ml @19:54:45.95 delay 4 seconds @19:54:49.97 ATV.dial ARV @19:54:50.53 delay 2 seconds @19:54:52.55 ARV.dial :carrier @19:54:52.56 delay 2 seconds @19:54:54.64 AS.seek 0.65ml @19:55:45.10 delay 4 seconds @19:55:49.12 ATV.dial PCR @19:55:49.91 ASV.dial :waste1 @19:55:49.92 ARV.dial :air1 @19:55:50.36 Positioning Slug by 0.324ml @19:55:50.45 AS.seek 0.326ml @20:01:16.72 delay 5 seconds @20:01:21.74 ATV.dial ARV @20:01:22.52 ASV.dial :PCRlock @20:01:22.92 delay 5 seconds @20:01:27.94 Running Rp Profile60C300 thermocycling @20:01:27.95 PCR.program Profile60C300 @20:01:28.10 Profile60C300 should complete in < 1:45:21 @20:01:28.11 PCR.run 2:16:41.25 @21:23:53.07 PCR.off @21:23:54.58 PCR for Rp completed -- closed /var/log/esp/Rp18dec1119h.pcr @21:23:54.66 Cleaning PCR @21:23:54.67 AFV.select :carrierSolution @21:23:54.79 delay 2 seconds @21:23:56.82 AS.reconfigure ASconfig @21:23:56.87 AS.seek 0.5ml @21:24:07.18 delay 1 second @21:24:08.20 AFV.select :holdingCoil @21:24:08.30 ASV.dial :waste1 @21:24:08.79 ATV.dial :air3 @21:24:10.07 AS.reconfigure ASslow1 @21:24:10.14 AS.seek 0.51ml @21:24:12.41 delay 2 seconds @21:24:14.43 ATV.dial :bleach @21:24:15.05 AS.seek 0.81ml @21:25:15.65 delay 3 seconds @21:25:18.67 ATV.dial :air3 @21:25:19.24 delay 3 seconds @21:25:22.31 AS.seek 0.835ml @21:25:27.71 delay 3 seconds @21:25:30.73 ATV.dial :bleach @21:25:31.35 AS.seek 0.82ml @21:25:34.60 delay 3 seconds @21:25:37.62 ARV.dial :waste @21:25:38.24 delay 3 seconds @21:25:41.26 ATV.dial :ARV @21:25:42.15 AS.reconfigure ASconfig @21:25:42.21 AS.seek 0.47ml @21:26:02.62 AFV.select :carrierSolution @21:26:02.74 delay 2 seconds @21:26:04.77 AS.seek 1ml @21:26:35.54 delay 1 second @21:26:36.56 AFV.select :holdingCoil @21:26:36.66 delay 1 second @21:26:37.68 AS.reconfigure ASfast @21:26:37.74 AS.seek 0ml @21:27:05.10 AFV.select :carrierSolution @21:27:05.21 delay 2 seconds @21:27:07.23 AS.reconfigure ASconfig @21:27:07.29 AS.seek 1ml @21:28:05.08 delay 1 second @21:28:06.10 AFV.select :holdingCoil @21:28:06.20 delay 1 second @21:28:07.23 AS.reconfigure ASfast @21:28:07.28 AS.seek 0ml @21:28:34.66 AFV.select :carrierSolution @21:28:34.77 delay 2 seconds @21:28:36.79 AS.reconfigure ASconfig @21:28:36.85 AS.seek 1ml @21:29:34.61 delay 1 second @21:29:35.63 AFV.select :holdingCoil @21:29:35.73 delay 1 second @21:29:36.76 AS.reconfigure ASfast @21:29:36.81 AS.seek 0ml @21:30:04.16 ARV.dial between carrier and air1 @21:30:04.68 ATV.dial :air3 @21:30:05.98 AS.reconfigure ASslow1 @21:30:06.04 AS.seek 0.01ml @21:30:08.39 delay 2 seconds @21:30:10.41 ATV.dial :bleach @21:30:11.42 AS.seek 0.81ml @21:32:52.58 delay 3 seconds @21:32:55.60 ATV.dial :air3 @21:32:56.18 delay 3 seconds @21:32:59.25 AS.seek 0.835ml @21:33:04.55 delay 3 seconds @21:33:07.57 ATV.dial :bleach @21:33:08.19 AS.seek 0.82ml @21:33:11.44 delay 3 seconds @21:33:14.46 ATV.dial PCR @21:33:14.86 AS.reconfigure ASconfig @21:33:14.92 AS.seek :empty @21:34:02.41 PCR.program :bleachProfile @21:34:02.46 PCR program should complete in < 2:45 @21:34:02.47 PCR.run 10:00 @21:36:58.25 PCR.off @21:36:58.28 AFV.select :carrierSolution @21:36:58.40 delay 2 seconds @21:37:00.43 AS.seek 1ml @21:37:58.27 delay 1 second @21:37:59.29 AFV.select :holdingCoil @21:37:59.39 delay 1 second @21:38:00.42 AS.reconfigure ASfast @21:38:00.47 AS.seek 0ml @21:38:27.83 delay 3 seconds @21:38:30.85 AFV.select :carrierSolution @21:38:30.95 delay 2 seconds @21:38:32.97 AS.reconfigure ASconfig @21:38:33.04 AS.seek 1ml @21:39:30.85 delay 1 second @21:39:31.87 AFV.select :holdingCoil @21:39:31.97 delay 1 second @21:39:33.00 AS.reconfigure ASfast @21:39:33.05 AS.seek 0ml @21:40:00.41 delay 3 seconds @21:40:03.43 AFV.select :carrierSolution @21:40:03.53 delay 2 seconds @21:40:05.55 AS.reconfigure ASconfig @21:40:05.62 AS.seek 1ml @21:41:03.43 delay 1 second @21:41:04.45 AFV.select :holdingCoil @21:41:04.55 delay 1 second @21:41:05.58 AS.reconfigure ASfast @21:41:05.63 AS.seek 0ml @21:41:32.97 delay 3 seconds @21:41:35.99 AFV.select :carrierSolution @21:41:36.09 delay 2 seconds @21:41:38.11 AS.reconfigure ASconfig @21:41:38.18 AS.seek 1ml @21:42:35.97 delay 1 second @21:42:36.99 AFV.select :holdingCoil @21:42:37.09 delay 1 second @21:42:38.12 AS.reconfigure ASfast @21:42:38.17 AS.seek 0ml @21:43:05.54 delay 3 seconds @21:43:08.56 AFV.select :carrierSolution @21:43:08.66 delay 2 seconds @21:43:10.69 AS.reconfigure ASconfig @21:43:10.75 AS.seek 1ml @21:44:08.54 delay 1 second @21:44:09.56 AFV.select :holdingCoil @21:44:09.66 delay 1 second @21:44:10.68 AS.reconfigure ASnorm @21:44:10.74 AS.seek 0ml @21:45:01.47
AFV.select :carrierSolution @21:45:01.58 delay 2 seconds @21:45:03.61 AS.reconfigure ASconfig @21:45:03.66 AS.seek 0.5ml @21:45:32.71 delay 1 second @21:45:33.73 AFV.select :holdingCoil @21:45:33.83 ATV.dial :air1 @21:45:35.33 AS.reconfigure ASslow2 @21:45:35.39 AS.seek 0.506ml @21:45:41.66 ATV.dial :extSrc @21:45:43.38 AS.seek 0.512ml @21:45:49.60 delay 5 seconds @21:45:54.62 ATV.dial :air1 @21:45:56.35 AS.reconfigure ASslow1 @21:45:56.41 AS.seek 0.542ml @21:46:02.71 ATV.dial :waste @21:46:03.27 AS.reconfigure ASconfig @21:46:03.33 AS.seek :empty @21:46:35.00 Setting up PCR for Rp -- writing /var/log/esp/Rp18dec1121h.pcr @21:46:35.02 Building PCR Slug from extSrc with MM2 mix, PP3 primer @21:46:35.05 Priming with carrier @21:46:35.07 ATV.dial ARV @21:46:36.57 ARV.dial :carrier @21:46:36.92 AS.reconfigure ASnorm @21:46:36.98 AS.seek 0.499ml @21:47:02.55 AFV.select :carrierSolution @21:47:02.66 delay 2 seconds @21:47:04.69 AS.reconfigure ASconfig @21:47:04.74 AS.seek 1ml @21:47:33.82 delay 1 second @21:47:34.84 AFV.select :holdingCoil @21:47:34.94 delay 1 second @21:47:35.96 ATV.dial :waste @21:47:37.63 AS.reconfigure ASnorm @21:47:37.69 AS.seek :empty @21:48:28.44 AFV.select :carrierSolution @21:48:28.93 delay 2 seconds @21:48:30.96 AS.reconfigure ASconfig @21:48:31.01 AS.seek 0.5ml @21:49:00.15 delay 1 second @21:49:01.17 AFV.select :holdingCoil @21:49:01.27 Building Slug @21:49:01.28 ATV.dial :ARV @21:49:03.05 delay 2 seconds @21:49:05.07 ARV.dial :air1 @21:49:05.47 delay 2 seconds @21:49:07.54 AS.reconfigure ASslow2 @21:49:07.60 AS.seek 0.506ml @21:49:13.82 delay 2 seconds @21:49:15.84 ARV.dial :MM2 @21:49:17.36 delay 2 seconds @21:49:19.38 Adding MM2 @21:49:19.46 AS.seek 0.524ml @21:49:37.76 delay 25 seconds @21:50:02.78 ARV.dial :PP3 @21:50:03.19 Adding PP3 @21:50:03.20 delay 2 seconds @21:50:05.28 AS.seek 0.53ml @21:50:11.77 delay 10 seconds @21:50:21.80 ARV.dial :air2 @21:50:22.21 adding ARV air @21:50:22.22 delay 2 seconds @21:50:24.30 AS.seek 0.536ml @21:50:30.54 delay 5 seconds @21:50:35.56 ARV.dial :carrier @21:50:36.88 delay 7 seconds @21:50:43.96 AS.seek 0.594ml @21:51:42.54 delay 5 seconds @21:51:47.56 ATV.dial :extSrc @21:51:48.13 delay 2 seconds @21:51:50.20 AS.seek 0.6ml @21:51:56.58 delay 4 seconds @21:52:00.60 ATV.dial ARV @21:52:01.16 delay 2 seconds @21:52:03.18 ARV.dial :carrier @21:52:03.19 delay 2 seconds @21:52:05.27 AS.seek 0.65ml @21:52:55.80 delay 4 seconds @21:52:59.82 ATV.dial PCR @21:53:00.60 ASV.dial :waste1 @21:53:00.61 ARV.dial :air1 @21:53:01.05 Positioning Slug by 0.324ml @21:53:01.14 AS.seek 0.326ml @21:58:27.40 delay 5 seconds @21:58:32.42 ATV.dial ARV @21:58:33.21 ASV.dial :PCRlock @21:58:33.60 delay 5 seconds @21:58:38.62 Running Rp Profile60C300 thermocycling @21:58:38.63 PCR.program Profile60C300 @21:58:38.78 Profile60C300 should complete in < 1:45:21 @21:58:38.79 PCR.run 2:16:41.25 @23:21:11.02 PCR.off @23:21:12.54 PCR for Rp completed -- closed /var/log/esp/Rp18dec1121h.pcr @23:21:12.62 Cleaning PCR @23:21:12.63 AFV.select :carrierSolution @23:21:12.76 delay 2 seconds @23:21:14.79 AS.reconfigure ASconfig @23:21:14.84 AS.seek 0.5ml @23:21:25.15 delay 1 second @23:21:26.17 AFV.select :holdingCoil @23:21:26.27 ASV.dial :waste1 @23:21:26.65 ATV.dial :air3 @23:21:27.93 AS.reconfigure ASslow1 @23:21:28.00 AS.seek 0.51ml @23:21:30.27 delay 2 seconds @23:21:32.29 ATV.dial :bleach @23:21:32.90 AS.seek 0.81ml @23:22:33.49 delay 3 seconds @23:22:36.51 ATV.dial :air3 @23:22:37.07 delay 3 seconds @23:22:40.14 AS.seek 0.835ml @23:22:45.45 delay 3 seconds @23:22:48.47 ATV.dial :bleach @23:22:49.10 AS.seek 0.82ml @23:22:52.43 delay 3 seconds @23:22:55.45 ARV.dial :waste @23:22:56.07 delay 3 seconds @23:22:59.09 ATV.dial :ARV @23:22:59.98 AS.reconfigure ASconfig @23:23:00.05 AS.seek 0.47ml @23:23:20.46 AFV.select :carrierSolution @23:23:20.57 delay 2 seconds @23:23:22.60 AS.seek 1ml @23:23:53.37 delay 1 second @23:23:54.39 AFV.select :holdingCoil @23:23:54.49 delay 1 second @23:23:55.52 AS.reconfigure ASfast @23:23:55.57 AS.seek 0ml @23:24:22.93 AFV.select :carrierSolution @23:24:23.04 delay 2 seconds @23:24:25.06 AS.reconfigure ASconfig @23:24:25.12 AS.seek 1ml @23:25:22.91 delay 1 second @23:25:23.93 AFV.select :holdingCoil @23:25:24.03 delay 1 second @23:25:25.06 AS.reconfigure ASfast @23:25:25.11 AS.seek 0ml @23:25:52.48 AFV.select :carrierSolution @23:25:52.59 delay 2 seconds @23:25:54.61 AS.reconfigure ASconfig @23:25:54.67 AS.seek 1ml @23:26:52.46 delay 1 second @23:26:53.48 AFV.select :holdingCoil @23:26:53.58 delay 1 second @23:26:54.60 AS.reconfigure ASfast @23:26:54.66 AS.seek 0ml @23:27:22.02 ARV.dial between carrier and air1 @23:27:22.54 ATV.dial :air3 @23:27:23.82 AS.reconfigure ASslow1 @23:27:23.88 AS.seek 0.01ml @23:27:26.24 delay 2 seconds @23:27:28.26 ATV.dial :bleach @23:27:28.90 AS.seek 0.81ml @23:30:10.04 delay 3 seconds @23:30:13.06 ATV.dial :air3 @23:30:13.63 delay 3 seconds @23:30:16.70 AS.seek 0.835ml @23:30:22.08 delay 3 seconds @23:30:25.10 ATV.dial :bleach @23:30:25.74 AS.seek 0.82ml @23:30:29.04 delay 3 seconds @23:30:32.06 ATV.dial PCR @23:30:32.46 AS.reconfigure ASconfig @23:30:32.52 AS.seek :empty @23:31:19.99 PCR.program :bleachProfile @23:31:20.04 PCR program should complete in < 2:45 @23:31:20.05 PCR.run 10:00 @23:34:16.21 PCR.off @23:34:16.24 AFV.select :carrierSolution @23:34:16.36 delay 2 seconds @23:34:18.39 AS.seek 1ml @23:35:16.23 delay 1 second @23:35:17.25 AFV.select :holdingCoil @23:35:17.35 delay 1 second @23:35:18.38 AS.reconfigure ASfast @23:35:18.43 AS.seek 0ml @23:35:45.79 delay 3 seconds @23:35:48.81 AFV.select :carrierSolution @23:35:48.91 delay 2 seconds @23:35:50.93 AS.reconfigure ASconfig @23:35:51.01 AS.seek 1ml @23:36:48.81 delay 1 second @23:36:49.83 AFV.select :holdingCoil @23:36:49.93 delay 1 second @23:36:50.96 AS.reconfigure ASfast @23:36:51.01 AS.seek 0ml @23:37:18.46 delay 3 seconds @23:37:21.48 AFV.select :carrierSolution @23:37:21.58 delay 2 seconds @23:37:23.60 AS.reconfigure ASconfig @23:37:23.67 AS.seek 1ml @23:38:21.46 delay 1 second @23:38:22.48 AFV.select :holdingCoil @23:38:22.58 delay 1 second @23:38:23.61 AS.reconfigure ASfast @23:38:23.66 AS.seek 0ml @23:38:51.01 delay 3 seconds @23:38:54.03 AFV.select :carrierSolution @23:38:54.13 delay 2 seconds @23:38:56.15 AS.reconfigure ASconfig @23:38:56.22 AS.seek 1ml @23:39:54.02 delay 1 second @23:39:55.04 AFV.select :holdingCoil @23:39:55.14 delay 1 second @23:39:56.17 AS.reconfigure ASfast @23:39:56.22 AS.seek 0ml @23:40:23.59 delay 3 seconds @23:40:26.61 AFV.select :carrierSolution @23:40:26.71 delay 2 seconds @23:40:28.73 AS.reconfigure ASconfig @23:40:28.80 AS.seek 1ml @23:41:26.59 delay 1 second @23:41:27.61 AFV.select :holdingCoil @23:41:27.71 delay 1 second @23:41:28.74 AS.reconfigure ASnorm @23:41:28.79 AS.seek 0ml @23:42:19.52
AFV.select :carrierSolution @23:42:19.63 delay 2 seconds @23:42:21.65 AS.reconfigure ASconfig @23:42:21.71 AS.seek 0.5ml @23:42:50.79 delay 1 second @23:42:51.81 AFV.select :holdingCoil @23:42:51.91 ATV.dial :air1 @23:42:53.41 AS.reconfigure ASslow1 @23:42:53.47 AS.seek 0.52ml @23:42:57.77 delay 5 seconds @23:43:02.79 ATV.dial :extSrc @23:43:04.59 AS.reconfigure ASslow2 @23:43:04.65 AS.seek 0.545ml @23:43:30.15 delay 10 seconds @23:43:40.18 ATV.dial :air1 @23:43:41.89 AS.reconfigure ASslow1 @23:43:41.95 AS.seek 0.575ml @23:43:48.32 ATV.dial :waste @23:43:48.88 AS.reconfigure ASconfig @23:43:48.94 AS.seek :empty @23:44:22.49 Setting up PCR for Rp -- writing /var/log/esp/Rp18dec1123h.pcr @23:44:22.51 Building PCR Slug from extSrc with MM2 mix, PP3 primer @23:44:22.54 Priming with carrier @23:44:22.56 ATV.dial ARV @23:44:24.06 ARV.dial :carrier @23:44:24.43 AS.reconfigure ASnorm @23:44:24.49 AS.seek 0.499ml @23:44:50.07 AFV.select :carrierSolution @23:44:50.18 delay 2 seconds @23:44:52.21 AS.reconfigure ASconfig @23:44:52.27 AS.seek 1ml @23:45:21.35 delay 1 second @23:45:22.37 AFV.select :holdingCoil @23:45:22.47 delay 1 second @23:45:23.49 ATV.dial :waste @23:45:25.16 AS.reconfigure ASnorm @23:45:25.22 AS.seek :empty @23:46:15.99 AFV.select :carrierSolution @23:46:16.10 delay 2 seconds @23:46:18.13 AS.reconfigure ASconfig @23:46:18.18 AS.seek 0.5ml @23:46:47.27 delay 1 second @23:46:48.29 AFV.select :holdingCoil @23:46:48.39 Building Slug @23:46:48.40 ATV.dial :ARV @23:46:50.16 delay 2 seconds @23:46:52.18 ARV.dial :air1 @23:46:52.59 delay 2 seconds @23:46:54.66 AS.reconfigure ASslow2 @23:46:54.72 AS.seek 0.506ml @23:47:00.98 delay 2 seconds @23:47:03.00 ARV.dial :MM2 @23:47:04.52 delay 2 seconds @23:47:06.54 Adding MM2 @23:47:06.62 AS.seek 0.524ml @23:47:24.84 delay 25 seconds @23:47:49.86 ARV.dial :PP3 @23:47:50.27 Adding PP3 @23:47:50.64 delay 2 seconds @23:47:52.75 AS.seek 0.53ml @23:47:59.23 delay 10 seconds @23:48:09.26 ARV.dial :air2 @23:48:09.66 adding ARV air @23:48:09.67 delay 2 seconds @23:48:11.75 AS.seek 0.536ml @23:48:17.93 delay 5 seconds @23:48:22.95 ARV.dial :carrier @23:48:24.31 delay 7 seconds @23:48:31.39 AS.seek 0.594ml @23:49:29.90 delay 5 seconds @23:49:34.92 ATV.dial :extSrc @23:49:35.59 delay 2 seconds @23:49:37.66 AS.seek 0.6ml @23:49:43.87 delay 4 seconds @23:49:47.89 ATV.dial ARV @23:49:48.43 delay 2 seconds @23:49:50.45 ARV.dial :carrier @23:49:50.46 delay 2 seconds @23:49:52.54 AS.seek 0.65ml @23:50:43.07 delay 4 seconds @23:50:47.09 ATV.dial PCR @23:50:47.87 ASV.dial :waste1 @23:50:47.88 ARV.dial :air1 @23:50:48.32 Positioning Slug by 0.324ml @23:50:48.41 AS.seek 0.326ml @23:56:14.40 delay 5 seconds @23:56:19.42 ATV.dial ARV @23:56:20.21 ASV.dial :PCRlock @23:56:20.60 delay 5 seconds @23:56:25.62 Running Rp Profile60C300 thermocycling @23:56:25.63 PCR.program Profile60C300 @23:56:25.78 Profile60C300 should complete in < 1:45:21 @23:56:25.79 PCR.run 2:16:41.25 @01:18:51.60CET12-Dec-18 PCR.off @01:18:53.50 PCR for Rp completed -- closed /var/log/esp/Rp18dec1123h.pcr @01:18:53.57 Cleaning PCR @01:18:53.59 AFV.select :carrierSolution @01:18:53.70 delay 2 seconds @01:18:55.73 AS.reconfigure ASconfig @01:18:55.78 AS.seek 0.5ml @01:19:06.09 delay 1 second @01:19:07.11 AFV.select :holdingCoil @01:19:07.21 ASV.dial :waste1 @01:19:07.61 ATV.dial :air3 @01:19:08.92 AS.reconfigure ASslow1 @01:19:08.98 AS.seek 0.51ml @01:19:11.29 delay 2 seconds @01:19:13.31 ATV.dial :bleach @01:19:13.93 AS.seek 0.81ml @01:20:14.53 delay 3 seconds @01:20:17.55 ATV.dial :air3 @01:20:18.11 delay 3 seconds @01:20:21.18 AS.seek 0.835ml @01:20:26.53 delay 3 seconds @01:20:29.55 ATV.dial :bleach @01:20:30.17 AS.seek 0.82ml @01:20:33.51 delay 3 seconds @01:20:36.53 ARV.dial :waste @01:20:37.15 delay 3 seconds @01:20:40.17 ATV.dial :ARV @01:20:41.06 AS.reconfigure ASconfig @01:20:41.13 AS.seek 0.47ml @01:21:01.53 AFV.select :carrierSolution @01:21:01.65 delay 2 seconds @01:21:03.68 AS.seek 1ml @01:21:34.45 delay 1 second @01:21:35.47 AFV.select :holdingCoil @01:21:35.57 delay 1 second @01:21:36.60 AS.reconfigure ASfast @01:21:36.65 AS.seek 0ml @01:22:04.01 AFV.select :carrierSolution @01:22:04.12 delay 2 seconds @01:22:06.14 AS.reconfigure ASconfig @01:22:06.20 AS.seek 1ml @01:23:04.00 delay 1 second @01:23:05.02 AFV.select :holdingCoil @01:23:05.12 delay 1 second @01:23:06.15 AS.reconfigure ASfast @01:23:06.20 AS.seek 0ml @01:23:33.57 AFV.select :carrierSolution @01:23:33.68 delay 2 seconds @01:23:35.70 AS.reconfigure ASconfig @01:23:35.76 AS.seek 1ml @01:24:33.54 delay 1 second @01:24:34.56 AFV.select :holdingCoil @01:24:34.66 delay 1 second @01:24:35.69 AS.reconfigure ASfast @01:24:35.74 AS.seek 0ml @01:25:03.12 ARV.dial between carrier and air1 @01:25:03.64 ATV.dial :air3 @01:25:04.92 AS.reconfigure ASslow1 @01:25:04.99 AS.seek 0.01ml @01:25:07.32 delay 2 seconds @01:25:09.34 ATV.dial :bleach @01:25:09.96 AS.seek 0.81ml @01:27:51.12 delay 3 seconds @01:27:54.14 ATV.dial :air3 @01:27:54.70 delay 3 seconds @01:27:57.77 AS.seek 0.835ml @01:28:03.10 delay 3 seconds @01:28:06.12 ATV.dial :bleach @01:28:06.74 AS.seek 0.82ml @01:28:10.00 delay 3 seconds @01:28:13.02 ATV.dial PCR @01:28:13.42 AS.reconfigure ASconfig @01:28:13.48 AS.seek :empty @01:29:00.95 PCR.program :bleachProfile @01:29:01.00 PCR program should complete in < 2:45 @01:29:01.01 PCR.run 10:00 @01:31:56.79 PCR.off @01:31:56.82 AFV.select :carrierSolution @01:31:56.94 delay 2 seconds @01:31:58.97 AS.seek 1ml @01:32:56.79 delay 1 second @01:32:57.81 AFV.select :holdingCoil @01:32:57.92 delay 1 second @01:32:58.94 AS.reconfigure ASfast @01:32:59.00 AS.seek 0ml @01:33:26.36 delay 3 seconds @01:33:29.38 AFV.select :carrierSolution @01:33:29.48 delay 2 seconds @01:33:31.50 AS.reconfigure ASconfig @01:33:31.57 AS.seek 1ml @01:34:29.42 delay 1 second @01:34:30.44 AFV.select :holdingCoil @01:34:30.54 delay 1 second @01:34:31.57 AS.reconfigure ASfast @01:34:32.02 AS.seek 0ml @01:34:59.45 delay 3 seconds @01:35:02.47 AFV.select :carrierSolution @01:35:02.57 delay 2 seconds @01:35:04.59 AS.reconfigure ASconfig @01:35:04.67 AS.seek 1ml @01:36:02.48 delay 1 second @01:36:03.50 AFV.select :holdingCoil @01:36:03.60 delay 1 second @01:36:04.63 AS.reconfigure ASfast @01:36:04.68 AS.seek 0ml @01:36:32.06 delay 3 seconds @01:36:35.08 AFV.select :carrierSolution @01:36:35.18 delay 2 seconds @01:36:37.20 AS.reconfigure ASconfig @01:36:37.27 AS.seek 1ml @01:37:35.07 delay 1 second @01:37:36.09 AFV.select :holdingCoil @01:37:36.19 delay 1 second @01:37:37.22 AS.reconfigure ASfast @01:37:37.27 AS.seek 0ml @01:38:04.64 delay 3 seconds @01:38:07.66 AFV.select :carrierSolution @01:38:07.76 delay 2 seconds @01:38:09.78 AS.reconfigure ASconfig @01:38:09.85 AS.seek 1ml @01:39:07.65 delay 1 second @01:39:08.67 AFV.select :holdingCoil @01:39:08.77 delay 1 second @01:39:09.80 AS.reconfigure ASnorm @01:39:09.85 AS.seek 0ml @01:40:00.57
AFV.select :carrierSolution @01:40:00.68 delay 2 seconds @01:40:02.71 AS.reconfigure ASconfig @01:40:02.76 AS.seek 0.5ml @01:40:31.82 delay 1 second @01:40:32.84 AFV.select :holdingCoil @01:40:32.94 ATV.dial :air1 @01:40:34.46 AS.reconfigure ASslow2 @01:40:34.52 AS.seek 0.506ml @01:40:40.75 ATV.dial :extSrc @01:40:42.46 AS.seek 0.512ml @01:40:48.84 delay 5 seconds @01:40:53.86 ATV.dial :air1 @01:40:55.60 AS.reconfigure ASslow1 @01:40:55.65 AS.seek 0.542ml @01:41:01.96 ATV.dial :waste @01:41:02.53 AS.reconfigure ASconfig @01:41:02.59 AS.seek :empty @01:41:34.25 Setting up PCR for Rp -- writing /var/log/esp/Rp18dec1201h.pcr @01:41:34.27 Building PCR Slug from extSrc with MM2 mix, PP3 primer @01:41:34.30 Priming with carrier @01:41:34.31 ATV.dial ARV @01:41:35.93 ARV.dial :carrier @01:41:36.29 AS.reconfigure ASnorm @01:41:36.35 AS.seek 0.499ml @01:42:01.92 AFV.select :carrierSolution @01:42:02.03 delay 2 seconds @01:42:04.06 AS.reconfigure ASconfig @01:42:04.12 AS.seek 1ml @01:42:33.18 delay 1 second @01:42:34.20 AFV.select :holdingCoil @01:42:34.30 delay 1 second @01:42:35.32 ATV.dial :waste @01:42:37.00 AS.reconfigure ASnorm @01:42:37.06 AS.seek :empty @01:43:27.84 AFV.select :carrierSolution @01:43:27.95 delay 2 seconds @01:43:29.98 AS.reconfigure ASconfig @01:43:30.03 AS.seek 0.5ml @01:43:59.15 delay 1 second @01:44:00.17 AFV.select :holdingCoil @01:44:00.27 Building Slug @01:44:00.28 ATV.dial :ARV @01:44:01.95 delay 2 seconds @01:44:03.97 ARV.dial :air1 @01:44:04.37 delay 2 seconds @01:44:06.44 AS.reconfigure ASslow2 @01:44:06.50 AS.seek 0.506ml @01:44:12.71 delay 2 seconds @01:44:14.73 ARV.dial :MM2 @01:44:16.25 delay 2 seconds @01:44:18.27 Adding MM2 @01:44:18.36 AS.seek 0.524ml @01:44:36.75 delay 25 seconds @01:45:01.77 ARV.dial :PP3 @01:45:02.18 Adding PP3 @01:45:02.19 delay 2 seconds @01:45:04.27 AS.seek 0.53ml @01:45:10.51 delay 10 seconds @01:45:20.54 ARV.dial :air2 @01:45:20.95 adding ARV air @01:45:20.96 delay 2 seconds @01:45:23.04 AS.seek 0.536ml @01:45:29.26 delay 5 seconds @01:45:34.28 ARV.dial :carrier @01:45:35.61 delay 7 seconds @01:45:42.69 AS.seek 0.594ml @01:46:41.34 delay 5 seconds @01:46:46.36 ATV.dial :extSrc @01:46:46.95 delay 2 seconds @01:46:49.02 AS.seek 0.6ml @01:46:55.36 delay 4 seconds @01:46:59.38 ATV.dial ARV @01:46:59.95 delay 2 seconds @01:47:02.36 ARV.dial :carrier @01:47:02.37 delay 2 seconds @01:47:04.45 AS.seek 0.65ml @01:47:55.12 delay 4 seconds @01:47:59.14 ATV.dial PCR @01:47:59.92 ASV.dial :waste1 @01:47:59.93 ARV.dial :air1 @01:48:00.36 Positioning Slug by 0.324ml @01:48:00.45 AS.seek 0.326ml @01:53:26.51 delay 5 seconds @01:53:31.53 ATV.dial ARV @01:53:32.32 ASV.dial :PCRlock @01:53:32.72 delay 5 seconds @01:53:37.74 Running Rp Profile60C300 thermocycling @01:53:37.75 PCR.program Profile60C300 @01:53:37.89 Profile60C300 should complete in < 1:45:21 @01:53:37.90 PCR.run 2:16:41.25 @03:16:02.35 PCR.off @03:16:03.86 PCR for Rp completed -- closed /var/log/esp/Rp18dec1201h.pcr @03:16:03.94 Cleaning PCR @03:16:03.95 AFV.select :carrierSolution @03:16:04.07 delay 2 seconds @03:16:06.10 AS.reconfigure ASconfig @03:16:06.15 AS.seek 0.5ml @03:16:16.45 delay 1 second @03:16:17.47 AFV.select :holdingCoil @03:16:17.58 ASV.dial :waste1 @03:16:17.97 ATV.dial :air3 @03:16:19.25 AS.reconfigure ASslow1 @03:16:19.32 AS.seek 0.51ml @03:16:21.60 delay 2 seconds @03:16:23.62 ATV.dial :bleach @03:16:24.25 AS.seek 0.81ml @03:17:02.51 Session Ended @03:17:24.86 delay 3 seconds @03:17:27.88 ATV.dial :air3 @03:17:28.42 delay 3 seconds @03:17:31.49 AS.seek 0.835ml @03:17:37.25 delay 3 seconds @03:17:40.28 ATV.dial :bleach @03:17:40.89 AS.seek 0.82ml @03:17:44.12 delay 3 seconds @03:17:47.14 ARV.dial :waste @03:17:47.75 delay 3 seconds @03:17:50.77 ATV.dial :ARV @03:17:51.64 AS.reconfigure ASconfig @03:17:51.69 AS.seek 0.47ml @03:18:12.09 AFV.select :carrierSolution @03:18:12.19 delay 2 seconds @03:18:14.22 AS.seek 1ml @03:18:44.98 delay 1 second @03:18:46.00 AFV.select :holdingCoil @03:18:46.10 delay 1 second @03:18:47.13 AS.reconfigure ASfast @03:18:47.17 AS.seek 0ml @03:19:14.51 AFV.select :carrierSolution @03:19:14.61 delay 2 seconds @03:19:16.63 AS.reconfigure ASconfig @03:19:16.68 AS.seek 1ml @03:20:14.47 delay 1 second @03:20:15.49 AFV.select :holdingCoil @03:20:15.59 delay 1 second @03:20:16.62 AS.reconfigure ASfast @03:20:16.66 AS.seek 0ml @03:20:44.01 AFV.select :carrierSolution @03:20:44.11 delay 2 seconds @03:20:46.13 AS.reconfigure ASconfig @03:20:46.18 AS.seek 1ml @03:21:43.95 delay 1 second @03:21:44.97 AFV.select :holdingCoil @03:21:45.07 delay 1 second @03:21:46.10 AS.reconfigure ASfast @03:21:46.14 AS.seek 0ml @03:22:13.47 ARV.dial between carrier and air1 @03:22:13.97 ATV.dial :air3 @03:22:15.24 AS.reconfigure ASslow1 @03:22:15.29 AS.seek 0.01ml @03:22:17.58 delay 2 seconds @03:22:19.60 ATV.dial :bleach @03:22:20.20 AS.seek 0.81ml @03:25:01.36 delay 3 seconds @03:25:04.38 ATV.dial :air3 @03:25:04.92 delay 3 seconds @03:25:07.99 AS.seek 0.835ml @03:25:13.27 delay 3 seconds @03:25:16.29 ATV.dial :bleach @03:25:16.90 AS.seek 0.82ml @03:25:20.16 delay 3 seconds @03:25:23.18 ATV.dial PCR @03:25:23.58 AS.reconfigure ASconfig @03:25:23.62 AS.seek :empty @03:26:11.09 PCR.program :bleachProfile @03:26:11.14 PCR program should complete in < 2:45 @03:26:11.15 PCR.run 10:00 @03:29:06.92 PCR.off @03:29:06.95 AFV.select :carrierSolution @03:29:07.05 delay 2 seconds @03:29:09.08 AS.seek 1ml @03:30:06.91 delay 1 second @03:30:07.93 AFV.select :holdingCoil @03:30:08.03 delay 1 second @03:30:09.05 AS.reconfigure ASfast @03:30:09.10 AS.seek 0ml @03:30:36.45 delay 3 seconds @03:30:39.47 AFV.select :carrierSolution @03:30:39.57 delay 2 seconds @03:30:41.59 AS.reconfigure ASconfig @03:30:41.65 AS.seek 1ml @03:31:39.45 delay 1 second @03:31:40.47 AFV.select :holdingCoil @03:31:40.57 delay 1 second @03:31:41.59 AS.reconfigure ASfast @03:31:41.64 AS.seek 0ml @03:32:08.98 delay 3 seconds @03:32:12.00 AFV.select :carrierSolution @03:32:12.10 delay 2 seconds @03:32:14.12 AS.reconfigure ASconfig @03:32:14.17 AS.seek 1ml @03:33:11.94 delay 1 second @03:33:12.96 AFV.select :holdingCoil @03:33:13.06 delay 1 second @03:33:14.08 AS.reconfigure ASfast @03:33:14.13 AS.seek 0ml @03:33:41.48 delay 3 seconds @03:33:44.50 AFV.select :carrierSolution @03:33:44.60 delay 2 seconds @03:33:46.62 AS.reconfigure ASconfig @03:33:46.68 AS.seek 1ml @03:34:44.47 delay 1 second @03:34:45.49 AFV.select :holdingCoil @03:34:45.59 delay 1 second @03:34:46.61 AS.reconfigure ASfast @03:34:46.66 AS.seek 0ml @03:35:14.02 delay 3 seconds @03:35:17.04 AFV.select :carrierSolution @03:35:17.14 delay 2 seconds @03:35:19.16 AS.reconfigure ASconfig @03:35:19.21 AS.seek 1ml @03:36:17.03 delay 1 second @03:36:18.05 AFV.select :holdingCoil @03:36:18.15 delay 1 second @03:36:19.18 AS.reconfigure ASnorm @03:36:19.22 AS.seek 0ml @03:37:10.35
AFV.select :carrierSolution @03:37:10.45 delay 2 seconds @03:37:12.47 AS.reconfigure ASconfig @03:37:12.52 AS.seek 0.5ml @03:37:41.58 delay 1 second @03:37:42.60 AFV.select :holdingCoil @03:37:42.70 ATV.dial :air1 @03:37:44.20 AS.reconfigure ASslow1 @03:37:44.24 AS.seek 0.52ml @03:37:48.55 delay 5 seconds @03:37:53.57 ATV.dial :extSrc @03:37:55.28 AS.reconfigure ASslow2 @03:37:55.32 AS.seek 0.538ml @03:38:13.69 delay 10 seconds @03:38:23.72 ATV.dial :air1 @03:38:25.43 AS.reconfigure ASslow1 @03:38:25.47 AS.seek 0.568ml @03:38:31.81 ATV.dial :waste @03:38:32.36 AS.reconfigure ASconfig @03:38:32.40 AS.seek :empty @03:39:05.42 deconEluateRecoveryLine extSrc @03:39:05.43 ATV.dial :waste @03:39:05.44 AS.reconfigure ASnorm @03:39:05.48 AS.seek 0ml @03:39:05.81 ATV.dial :air3 @03:39:06.48 AS.reconfigure ASslow1 @03:39:06.52 AS.seek 0.01ml @03:39:08.78 delay 2 seconds @03:39:10.80 ATV.dial :bleach @03:39:11.26 AS.seek 0.31ml @03:40:11.86 delay 3 seconds @03:40:14.88 ATV.dial :air3 @03:40:15.44 delay 3 seconds @03:40:18.51 AS.seek 0.335ml @03:40:23.82 delay 3 seconds @03:40:26.84 ATV.dial :bleach @03:40:27.47 AS.seek 0.32ml @03:40:30.77 delay 3 seconds @03:40:33.79 ATV.dial :extSrc @03:40:34.42 AS.seek 0ml @03:41:39.03 AFV.select :carrierSolution @03:41:39.13 delay 2 seconds @03:41:41.16 AS.reconfigure ASconfig @03:41:41.20 AS.seek 0.5ml @03:42:10.23 delay 1 second @03:42:11.25 AFV.select :holdingCoil @03:42:11.35 ATV.dial :extSrc @03:42:11.37 AS.reconfigure ASslow1 @03:42:11.41 AS.seek 0ml @03:43:52.25 AFV.select :carrierSolution @03:43:52.35 delay 2 seconds @03:43:54.38 AS.reconfigure ASconfig @03:43:54.42 AS.seek 0.5ml @03:44:23.47 delay 1 second @03:44:24.49 AFV.select :holdingCoil @03:44:24.59 ATV.dial :extSrc @03:44:24.61 AS.reconfigure ASslow1 @03:44:24.65 AS.seek 0ml @03:46:05.44 AFV.select :carrierSolution @03:46:05.54 delay 2 seconds @03:46:07.57 AS.reconfigure ASconfig @03:46:07.61 AS.seek 0.5ml @03:46:36.67 delay 1 second @03:46:37.69 AFV.select :holdingCoil @03:46:37.79 ATV.dial :extSrc @03:46:37.81 AS.reconfigure ASslow1 @03:46:37.85 AS.seek 0ml @03:48:18.69 AFV.select :carrierSolution @03:48:18.79 delay 2 seconds @03:48:20.82 AS.reconfigure ASconfig @03:48:20.86 AS.seek 0.5ml @03:48:49.89 delay 1 second @03:48:50.91 AFV.select :holdingCoil @03:48:51.01 ATV.dial :extSrc @03:48:51.03 AS.reconfigure ASslow1 @03:48:51.07 AS.seek 0ml @03:50:31.91 ATV.dial :air2 @03:50:33.22 AS.reconfigure ASslow0 @03:50:33.26 AS.seek 0.5ml @03:51:23.87 ATV.dial :extSrc @03:51:25.34 AS.seek 0.02ml @03:52:13.92 delay 1 second @03:52:14.94 ATV.dial :waste @03:52:16.22 AS.reconfigure ASconfig @03:52:16.26 AS.seek :empty @03:52:17.77 deconEluateRecoveryLine extSrc @03:52:17.78 ATV.dial :waste @03:52:17.79 AS.reconfigure ASnorm @03:52:17.83 AS.seek 0ml @03:52:18.17 ATV.dial :air3 @03:52:19.01 AS.reconfigure ASslow1 @03:52:19.05 AS.seek 0.01ml @03:52:21.33 delay 2 seconds @03:52:23.35 ATV.dial :bleach @03:52:23.79 AS.seek 0.31ml @03:53:24.39 delay 3 seconds @03:53:27.41 ATV.dial :air3 @03:53:27.97 delay 3 seconds @03:53:31.04 AS.seek 0.335ml @03:53:36.34 delay 3 seconds @03:53:39.36 ATV.dial :bleach @03:53:39.98 AS.seek 0.32ml @03:53:43.28 delay 3 seconds @03:53:46.30 ATV.dial :extSrc @03:53:46.91 AS.seek 0ml @03:54:51.52 AFV.select :carrierSolution @03:54:51.62 delay 2 seconds @03:54:53.64 AS.reconfigure ASconfig @03:54:54.09 AS.seek 0.5ml @03:55:23.09 delay 1 second @03:55:24.11 AFV.select :holdingCoil @03:55:24.21 ATV.dial :extSrc @03:55:24.23 AS.reconfigure ASslow1 @03:55:24.27 AS.seek 0ml @03:57:05.13 AFV.select :carrierSolution @03:57:05.23 delay 2 seconds @03:57:07.26 AS.reconfigure ASconfig @03:57:07.30 AS.seek 0.5ml @03:57:36.34 delay 1 second @03:57:37.36 AFV.select :holdingCoil @03:57:37.46 ATV.dial :extSrc @03:57:37.48 AS.reconfigure ASslow1 @03:57:37.52 AS.seek 0ml @03:59:18.33 AFV.select :carrierSolution @03:59:18.43 delay 2 seconds @03:59:20.46 AS.reconfigure ASconfig @03:59:20.50 AS.seek 0.5ml @03:59:49.55 delay 1 second @03:59:50.57 AFV.select :holdingCoil @03:59:50.67 ATV.dial :extSrc @03:59:50.69 AS.reconfigure ASslow1 @03:59:50.73 AS.seek 0ml @04:01:31.55 AFV.select :carrierSolution @04:01:31.65 delay 2 seconds @04:01:33.68 AS.reconfigure ASconfig @04:01:33.72 AS.seek 0.5ml @04:02:02.75 delay 1 second @04:02:03.77 AFV.select :holdingCoil @04:02:03.87 ATV.dial :extSrc @04:02:03.89 AS.reconfigure ASslow1 @04:02:03.93 AS.seek 0ml @04:03:44.75 ATV.dial :air2 @04:03:46.08 AS.reconfigure ASslow0 @04:03:46.12 AS.seek 0.5ml @04:04:36.69 ATV.dial :extSrc @04:04:38.17 AS.seek 0.02ml @04:05:26.77 delay 1 second @04:05:27.79 ATV.dial :waste @04:05:29.08 AS.reconfigure ASconfig @04:05:29.12 AS.seek :empty @04:05:30.61 deconEluateRecoveryLine extSrc @04:05:30.62 ATV.dial :waste @04:05:30.63 AS.reconfigure ASnorm @04:05:30.67 AS.seek 0ml @04:05:31.05 ATV.dial :air3 @04:05:31.88 AS.reconfigure ASslow1 @04:05:31.92 AS.seek 0.01ml @04:05:34.19 delay 2 seconds @04:05:36.21 ATV.dial :bleach @04:05:36.65 AS.seek 0.31ml @04:06:37.25 delay 3 seconds @04:06:40.27 ATV.dial :air3 @04:06:40.83 delay 3 seconds @04:06:43.90 AS.seek 0.335ml @04:06:49.20 delay 3 seconds @04:06:52.22 ATV.dial :bleach @04:06:52.84 AS.seek 0.32ml @04:06:56.14 delay 3 seconds @04:06:59.16 ATV.dial :extSrc @04:06:59.77 AS.seek 0ml @04:08:04.39 AFV.select :carrierSolution @04:08:04.49 delay 2 seconds @04:08:06.52 AS.reconfigure ASconfig @04:08:06.56 AS.seek 0.5ml @04:08:35.59 delay 1 second @04:08:36.61 AFV.select :holdingCoil @04:08:36.71 ATV.dial :extSrc @04:08:36.73 AS.reconfigure ASslow1 @04:08:36.77 AS.seek 0ml @04:10:17.58 AFV.select :carrierSolution @04:10:17.68 delay 2 seconds @04:10:19.71 AS.reconfigure ASconfig @04:10:19.75 AS.seek 0.5ml @04:10:48.78 delay 1 second @04:10:49.80 AFV.select :holdingCoil @04:10:49.90 ATV.dial :extSrc @04:10:49.92 AS.reconfigure ASslow1 @04:10:49.96 AS.seek 0ml @04:12:30.78 AFV.select :carrierSolution @04:12:30.88 delay 2 seconds @04:12:32.90 AS.reconfigure ASconfig @04:12:32.95 AS.seek 0.5ml @04:13:01.98 delay 1 second @04:13:03.00 AFV.select :holdingCoil @04:13:03.10 ATV.dial :extSrc @04:13:03.12 AS.reconfigure ASslow1 @04:13:03.16 AS.seek 0ml @04:14:43.97 AFV.select :carrierSolution @04:14:44.07 delay 2 seconds @04:14:46.10 AS.reconfigure ASconfig @04:14:46.14 AS.seek 0.5ml @04:15:15.16 delay 1 second @04:15:16.18 AFV.select :holdingCoil @04:15:16.28 ATV.dial :extSrc @04:15:16.30 AS.reconfigure ASslow1 @04:15:16.34 AS.seek 0ml @04:16:57.16 ATV.dial :air2 @04:16:58.46 AS.reconfigure ASslow0 @04:16:58.51 AS.seek 0.5ml @04:17:49.11 ATV.dial :extSrc @04:17:50.59 AS.seek 0.02ml @04:18:39.16 delay 1 second @04:18:40.18 ATV.dial :waste @04:18:41.47 AS.reconfigure ASconfig @04:18:41.51 AS.seek :empty @15:18:03.21CET13-Dec-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @15:18:04.87 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @15:18:05.12 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @15:18:09.37 Polling Can every 10:00 @15:18:11.12 SPE.coast @15:18:11.17 SPE.configure SPEconfig @15:18:11.25 AS.reconfigure ASconfig @15:18:11.28 AS.hold @15:18:11.35 AS.configure ASconfig @15:18:11.43 SS.reconfigure SSconfig @15:18:11.47 SS.hold @15:18:11.51 SS.configure SSconfig @15:18:11.59 CC.reconfigure CCconfig @15:18:11.63 CC.hold @15:18:11.70 CC.configure CCconfig @15:18:11.79 CS.reconfigure CSconfig @15:18:11.82 CS.hold @15:18:11.89 CS.configure CSconfig @15:18:12.31 PC.reconfigure PCconfig @15:18:12.34 PC.hold @15:18:12.40 PC.configure PCconfig @15:18:12.48 PS.configure PSconfig @15:18:12.58 Forearm.reconfigure LooseForearm @15:18:12.62 Forearm.hold @15:18:12.68 Forearm.configure LooseForearm @15:18:12.77 Elbow.reconfigure LooseElbow @15:18:12.80 Elbow.hold @15:18:12.85 Elbow.configure LooseElbow @15:18:12.95 SC.reconfigure SCloose @15:18:12.99 SC.hold @15:18:13.03 SC.configure SCloose @15:18:13.11 SE.reconfigure SEconfig @15:18:13.15 SE.hold @15:18:13.21 SE.configure SEconfig @15:18:13.25 CH.coast @15:18:13.30 CH.configure CHconfig @15:18:13.33 PH.coast @15:18:13.38 PH.configure PHconfig @15:18:13.41 PCR.off @15:18:13.44 PCR.pidtemp= 50,0.1,0,300,25,25 @15:18:13.46 PCR.caltemp= 2.7073,0.0373,0 @15:18:13.49 All dwarf controllers running firmware version 3.75 @15:18:13.61 Cleanly exited at 15:12:39CET13-Dec-18 @15:18:13.64 Loading /home/esp/esp2/mission/stdMER2017_MWJ.rb mission @15:18:14.16 AFV.select :carrierSolution @15:18:14.26 delay 2 seconds @15:18:16.31 Session Begun @15:18:16.39
AS.seek 0.5ml @15:18:45.46 delay 1 second @15:18:46.48 AFV.select :holdingCoil @15:18:46.58 ATV.dial :extSrc @15:18:47.02 AS.reconfigure ASslow1 @15:18:47.08 AS.seek 0.53ml @15:18:53.39 delay 5 seconds @15:18:58.47 AS.reconfigure ASslow2 @15:18:58.53 AS.seek 0.54ml @15:19:08.74 delay 20 seconds @15:19:28.81 AS.reconfigure ASslow1 @15:19:28.87 AS.seek 0.57ml @15:19:35.18 delay 5 seconds @15:19:40.26 AS.reconfigure ASslow2 @15:19:40.32 AS.seek 0.58ml @15:19:50.54 delay 20 seconds @15:20:10.61 AS.reconfigure ASslow1 @15:20:10.67 AS.seek 0.61ml @15:20:16.96 delay 5 seconds @15:20:22.04 AS.reconfigure ASslow2 @15:20:22.10 AS.seek 0.62ml @15:20:32.41 delay 20 seconds @15:20:52.48 AS.reconfigure ASslow1 @15:20:52.54 AS.seek 0.65ml @15:20:58.85 delay 5 seconds @15:21:03.92 AS.reconfigure ASslow2 @15:21:03.98 AS.seek 0.66ml @15:21:14.30 delay 20 seconds @15:21:34.34 AS.reconfigure ASslow1 @15:21:34.39 AS.seek 0.705ml @15:21:43.79 delay 10 seconds @15:21:53.82 ATV.dial :air1 @15:21:55.56 AS.seek 0.725ml @15:21:59.86 delay 3 seconds @15:22:02.88 ATV.dial :waste @15:22:03.44 AS.reconfigure ASslow0 @15:22:03.50 AS.seek :empty @15:23:16.93 Setting up PCR for Ss -- writing /var/log/esp/Ss18dec1315h.pcr @15:23:16.95 Building PCR Slug from extSrc with MM1 mix, PP2 primer @15:23:16.98 Priming with carrier @15:23:17.00 ATV.dial ARV @15:23:18.52 ARV.dial :carrier @15:23:18.88 AS.reconfigure ASnorm @15:23:18.94 AS.seek 0.499ml @15:23:44.51 AFV.select :carrierSolution @15:23:44.63 delay 2 seconds @15:23:46.66 AS.reconfigure ASconfig @15:23:46.72 AS.seek 1ml @15:24:15.80 delay 1 second @15:24:16.82 AFV.select :holdingCoil @15:24:16.92 delay 1 second @15:24:17.94 ATV.dial :waste @15:24:19.63 AS.reconfigure ASnorm @15:24:19.69 AS.seek :empty @15:25:10.43 AFV.select :carrierSolution @15:25:10.55 delay 2 seconds @15:25:12.57 AS.reconfigure ASconfig @15:25:12.63 AS.seek 0.5ml @15:25:41.71 delay 1 second @15:25:42.73 AFV.select :holdingCoil @15:25:42.83 Building Slug @15:25:42.84 ATV.dial :ARV @15:25:44.50 delay 2 seconds @15:25:46.52 ARV.dial :air1 @15:25:46.94 delay 2 seconds @15:25:49.01 AS.reconfigure ASslow2 @15:25:49.07 AS.seek 0.506ml @15:25:55.52 delay 2 seconds @15:25:57.54 ARV.dial :MM1 @15:25:58.16 delay 2 seconds @15:26:00.18 Adding MM1 @15:26:00.26 AS.seek 0.524ml @15:26:18.79 delay 25 seconds @15:26:43.81 ARV.dial :PP2 @15:26:44.22 Adding PP2 @15:26:44.23 delay 2 seconds @15:26:46.31 AS.seek 0.53ml @15:26:52.75 delay 10 seconds @15:27:02.78 ARV.dial :air2 @15:27:03.21 adding ARV air @15:27:03.22 delay 2 seconds @15:27:05.30 AS.seek 0.536ml @15:27:11.52 delay 5 seconds @15:27:16.54 ARV.dial :carrier @15:27:17.91 delay 7 seconds @15:27:24.99 AS.seek 0.594ml @15:28:23.49 delay 5 seconds @15:28:28.51 ATV.dial :extSrc @15:28:29.08 delay 2 seconds @15:28:31.15 AS.seek 0.6ml @15:28:37.46 delay 4 seconds @15:28:41.48 ATV.dial ARV @15:28:42.04 delay 2 seconds @15:28:44.06 ARV.dial :carrier @15:28:44.07 delay 2 seconds @15:28:46.15 AS.seek 0.65ml @15:29:36.79 delay 4 seconds @15:29:40.81 ATV.dial PCR @15:29:41.58 ASV.dial :waste1 @15:29:41.68 ARV.dial :air1 @15:29:42.49 Positioning Slug by 0.324ml @15:29:42.58 AS.seek 0.326ml @15:35:08.69 delay 5 seconds @15:35:13.71 ATV.dial ARV @15:35:14.51 ASV.dial :PCRlock @15:35:14.93 delay 5 seconds @15:35:19.95 Running Ss Profile60C350 thermocycling @15:35:19.97 PCR.program Profile60C350 @15:35:20.12 Profile60C350 should complete in < 1:48:05 @15:35:20.13 PCR.run 2:20:06.25 @16:57:29.47 PCR.off @16:57:31.46 PCR for Ss completed -- closed /var/log/esp/Ss18dec1315h.pcr @16:57:31.54 Cleaning PCR @16:57:31.55 AFV.select :carrierSolution @16:57:31.66 delay 2 seconds @16:57:33.68 AS.reconfigure ASconfig @16:57:33.74 AS.seek 0.5ml @16:57:44.06 delay 1 second @16:57:45.08 AFV.select :holdingCoil @16:57:45.18 ASV.dial :waste1 @16:57:45.57 ATV.dial :air3 @16:57:46.87 AS.reconfigure ASslow1 @16:57:46.93 AS.seek 0.51ml @16:57:49.21 delay 2 seconds @16:57:51.24 ATV.dial :bleach @16:57:51.88 AS.seek 0.81ml @16:58:52.51 delay 3 seconds @16:58:55.53 ATV.dial :air3 @16:58:56.10 delay 3 seconds @16:58:59.17 AS.seek 0.835ml @16:59:04.46 delay 3 seconds @16:59:07.48 ATV.dial :bleach @16:59:08.12 AS.seek 0.82ml @16:59:11.34 delay 3 seconds @16:59:14.36 ARV.dial :waste @16:59:15.31 delay 3 seconds @16:59:18.39 ATV.dial :ARV @16:59:19.29 AS.reconfigure ASconfig @16:59:19.35 AS.seek 0.47ml @16:59:39.77 AFV.select :carrierSolution @16:59:39.89 delay 2 seconds @16:59:41.92 AS.seek 1ml @17:00:12.71 delay 1 second @17:00:13.73 AFV.select :holdingCoil @17:00:13.83 delay 1 second @17:00:14.86 AS.reconfigure ASfast @17:00:14.91 AS.seek 0ml @17:00:42.29 AFV.select :carrierSolution @17:00:42.40 delay 2 seconds @17:00:44.42 AS.reconfigure ASconfig @17:00:44.48 AS.seek 1ml @17:01:42.26 delay 1 second @17:01:43.28 AFV.select :holdingCoil @17:01:43.38 delay 1 second @17:01:44.40 AS.reconfigure ASfast @17:01:44.46 AS.seek 0ml @17:02:11.81 AFV.select :carrierSolution @17:02:11.93 delay 2 seconds @17:02:13.95 AS.reconfigure ASconfig @17:02:14.01 AS.seek 1ml @17:03:11.76 delay 1 second @17:03:12.78 AFV.select :holdingCoil @17:03:12.88 delay 1 second @17:03:13.90 AS.reconfigure ASfast @17:03:13.96 AS.seek 0ml @17:03:41.43 ARV.dial between carrier and air1 @17:03:41.95 ATV.dial :air3 @17:03:43.26 AS.reconfigure ASslow1 @17:03:43.32 AS.seek 0.01ml @17:03:45.70 delay 2 seconds @17:03:47.72 ATV.dial :bleach @17:03:48.35 AS.seek 0.81ml @17:06:29.49 delay 3 seconds @17:06:32.51 ATV.dial :air3 @17:06:33.07 delay 3 seconds @17:06:36.14 AS.seek 0.835ml @17:06:41.43 delay 3 seconds @17:06:44.45 ATV.dial :bleach @17:06:45.07 AS.seek 0.82ml @17:06:48.40 delay 3 seconds @17:06:51.42 ATV.dial PCR @17:06:51.84 AS.reconfigure ASconfig @17:06:51.90 AS.seek :empty @17:07:39.39 PCR.program :bleachProfile @17:07:39.45 PCR program should complete in < 2:45 @17:07:39.46 PCR.run 10:00 @17:10:38.26 PCR.off @17:10:38.29 AFV.select :carrierSolution @17:10:38.41 delay 2 seconds @17:10:40.44 AS.seek 1ml @17:11:38.28 delay 1 second @17:11:39.30 AFV.select :holdingCoil @17:11:39.41 delay 1 second @17:11:40.43 AS.reconfigure ASfast @17:11:40.49 AS.seek 0ml @17:12:07.87 delay 3 seconds @17:12:10.89 AFV.select :carrierSolution @17:12:11.00 delay 2 seconds @17:12:13.02 AS.reconfigure ASconfig @17:12:13.09 AS.seek 1ml @17:13:10.85 delay 1 second @17:13:11.87 AFV.select :holdingCoil @17:13:11.98 delay 1 second @17:13:13.00 AS.reconfigure ASfast @17:13:13.06 AS.seek 0ml @17:13:40.43 delay 3 seconds @17:13:43.45 AFV.select :carrierSolution @17:13:43.55 delay 2 seconds @17:13:45.57 AS.reconfigure ASconfig @17:13:45.64 AS.seek 1ml @17:14:43.43 delay 1 second @17:14:44.45 AFV.select :holdingCoil @17:14:44.55 delay 1 second @17:14:45.57 AS.reconfigure ASfast @17:14:45.63 AS.seek 0ml @17:15:13.01 delay 3 seconds @17:15:16.03 AFV.select :carrierSolution @17:15:16.14 delay 2 seconds @17:15:18.16 AS.reconfigure ASconfig @17:15:18.23 AS.seek 1ml @17:16:16.03 delay 1 second @17:16:17.05 AFV.select :holdingCoil @17:16:17.15 delay 1 second @17:16:18.17 AS.reconfigure ASfast @17:16:18.23 AS.seek 0ml @17:16:45.60 delay 3 seconds @17:16:48.62 AFV.select :carrierSolution @17:16:48.72 delay 2 seconds @17:16:50.74 AS.reconfigure ASconfig @17:16:50.82 AS.seek 1ml @17:17:48.59 delay 1 second @17:17:49.61 AFV.select :holdingCoil @17:17:49.72 delay 1 second @17:17:50.74 AS.reconfigure ASnorm @17:17:50.80 AS.seek 0ml @17:18:41.51
AFV.select :carrierSolution @17:18:41.62 delay 2 seconds @17:18:43.64 AS.reconfigure ASconfig @17:18:43.70 AS.seek 0.5ml @17:19:12.78 delay 1 second @17:19:13.80 AFV.select :holdingCoil @17:19:13.91 ATV.dial :air1 @17:19:15.42 AS.reconfigure ASslow2 @17:19:15.48 AS.seek 0.506ml @17:19:21.68 ATV.dial :extSrc @17:19:23.40 AS.seek 0.512ml @17:19:29.71 delay 5 seconds @17:19:34.73 ATV.dial :air1 @17:19:36.47 AS.reconfigure ASslow1 @17:19:36.53 AS.seek 0.542ml @17:19:42.82 ATV.dial :waste @17:19:43.39 AS.reconfigure ASconfig @17:19:43.45 AS.seek :empty @17:20:15.12 Setting up PCR for Ss -- writing /var/log/esp/Ss18dec1317h.pcr @17:20:15.15 Building PCR Slug from extSrc with MM1 mix, PP2 primer @17:20:15.18 Priming with carrier @17:20:15.20 ATV.dial ARV @17:20:16.70 ARV.dial :carrier @17:20:17.06 AS.reconfigure ASnorm @17:20:17.12 AS.seek 0.499ml @17:20:42.66 AFV.select :carrierSolution @17:20:42.78 delay 2 seconds @17:20:44.81 AS.reconfigure ASconfig @17:20:44.87 AS.seek 1ml @17:21:13.93 delay 1 second @17:21:14.95 AFV.select :holdingCoil @17:21:15.05 delay 1 second @17:21:16.07 ATV.dial :waste @17:21:17.76 AS.reconfigure ASnorm @17:21:17.82 AS.seek :empty @17:22:08.56 AFV.select :carrierSolution @17:22:08.68 delay 2 seconds @17:22:10.70 AS.reconfigure ASconfig @17:22:10.76 AS.seek 0.5ml @17:22:40.28 delay 1 second @17:22:41.30 AFV.select :holdingCoil @17:22:41.40 Building Slug @17:22:41.41 ATV.dial :ARV @17:22:43.09 delay 2 seconds @17:22:45.11 ARV.dial :air1 @17:22:45.51 delay 2 seconds @17:22:47.58 AS.reconfigure ASslow2 @17:22:47.65 AS.seek 0.506ml @17:22:54.28 delay 2 seconds @17:22:56.30 ARV.dial :MM1 @17:22:56.93 delay 2 seconds @17:22:58.95 Adding MM1 @17:22:59.03 AS.seek 0.524ml @17:23:17.29 delay 25 seconds @17:23:42.31 ARV.dial :PP2 @17:23:42.71 Adding PP2 @17:23:42.72 delay 2 seconds @17:23:44.80 AS.seek 0.53ml @17:23:51.00 delay 10 seconds @17:24:01.03 ARV.dial :air2 @17:24:01.43 adding ARV air @17:24:01.44 delay 2 seconds @17:24:03.52 AS.seek 0.536ml @17:24:09.82 delay 5 seconds @17:24:14.84 ARV.dial :carrier @17:24:16.17 delay 7 seconds @17:24:23.25 AS.seek 0.594ml @17:25:22.00 delay 5 seconds @17:25:27.02 ATV.dial :extSrc @17:25:27.70 delay 2 seconds @17:25:29.77 AS.seek 0.6ml @17:25:36.06 delay 4 seconds @17:25:40.08 ATV.dial ARV @17:25:40.73 delay 2 seconds @17:25:42.75 ARV.dial :carrier @17:25:42.76 delay 2 seconds @17:25:44.84 AS.seek 0.65ml @17:26:35.73 delay 4 seconds @17:26:39.75 ATV.dial PCR @17:26:40.53 ASV.dial :waste1 @17:26:40.54 ARV.dial :air1 @17:26:40.97 Positioning Slug by 0.324ml @17:26:41.06 AS.seek 0.326ml @17:32:07.06 delay 5 seconds @17:32:12.08 ATV.dial ARV @17:32:12.87 ASV.dial :PCRlock @17:32:13.28 delay 5 seconds @17:32:18.30 Running Ss Profile60C350 thermocycling @17:32:18.31 PCR.program Profile60C350 @17:32:18.46 Profile60C350 should complete in < 1:48:05 @17:32:18.47 PCR.run 2:20:06.25 @18:53:36.35 PCR.off @18:53:38.58 PCR for Ss completed -- closed /var/log/esp/Ss18dec1317h.pcr @18:53:38.65 Cleaning PCR @18:53:38.66 AFV.select :carrierSolution @18:53:38.78 delay 2 seconds @18:53:40.81 AS.reconfigure ASconfig @18:53:40.86 AS.seek 0.5ml @18:53:51.19 delay 1 second @18:53:52.21 AFV.select :holdingCoil @18:53:52.31 ASV.dial :waste1 @18:53:52.71 ATV.dial :air3 @18:53:54.01 AS.reconfigure ASslow1 @18:53:54.08 AS.seek 0.51ml @18:53:56.36 delay 2 seconds @18:53:58.38 ATV.dial :bleach @18:53:59.02 AS.seek 0.81ml @18:54:59.61 delay 3 seconds @18:55:02.63 ATV.dial :air3 @18:55:03.21 delay 3 seconds @18:55:06.28 AS.seek 0.835ml @18:55:11.56 delay 3 seconds @18:55:14.58 ATV.dial :bleach @18:55:15.22 AS.seek 0.82ml @18:55:18.53 delay 3 seconds @18:55:21.55 ARV.dial :waste @18:55:22.19 delay 3 seconds @18:55:25.21 ATV.dial :ARV @18:55:26.10 AS.reconfigure ASconfig @18:55:26.16 AS.seek 0.47ml @18:55:46.57 AFV.select :carrierSolution @18:55:46.69 delay 2 seconds @18:55:48.72 AS.seek 1ml @18:56:19.52 delay 1 second @18:56:20.54 AFV.select :holdingCoil @18:56:20.64 delay 1 second @18:56:21.66 AS.reconfigure ASfast @18:56:21.72 AS.seek 0ml @18:56:49.09 AFV.select :carrierSolution @18:56:49.21 delay 2 seconds @18:56:51.23 AS.reconfigure ASconfig @18:56:51.29 AS.seek 1ml @18:57:49.06 delay 1 second @18:57:50.08 AFV.select :holdingCoil @18:57:50.19 delay 1 second @18:57:51.21 AS.reconfigure ASfast @18:57:51.27 AS.seek 0ml @18:58:18.63 AFV.select :carrierSolution @18:58:18.75 delay 2 seconds @18:58:20.77 AS.reconfigure ASconfig @18:58:20.83 AS.seek 1ml @18:59:18.60 delay 1 second @18:59:19.62 AFV.select :holdingCoil @18:59:19.72 delay 1 second @18:59:20.74 AS.reconfigure ASfast @18:59:20.80 AS.seek 0ml @18:59:48.14 ARV.dial between carrier and air1 @18:59:48.66 ATV.dial :air3 @18:59:49.96 AS.reconfigure ASslow1 @18:59:50.02 AS.seek 0.01ml @18:59:52.33 delay 2 seconds @18:59:54.35 ATV.dial :bleach @18:59:54.98 AS.seek 0.81ml @19:02:36.13 delay 3 seconds @19:02:39.15 ATV.dial :air3 @19:02:39.72 delay 3 seconds @19:02:42.79 AS.seek 0.835ml @19:02:48.15 delay 3 seconds @19:02:51.17 ATV.dial :bleach @19:02:51.80 AS.seek 0.82ml @19:02:55.08 delay 3 seconds @19:02:58.10 ATV.dial PCR @19:02:58.52 AS.reconfigure ASconfig @19:02:58.58 AS.seek :empty @19:03:46.05 PCR.program :bleachProfile @19:03:46.11 PCR program should complete in < 2:45 @19:03:46.12 PCR.run 10:00 @19:06:44.93 PCR.off @19:06:44.96 AFV.select :carrierSolution @19:06:45.07 delay 2 seconds @19:06:47.10 AS.seek 1ml @19:07:44.96 delay 1 second @19:07:45.98 AFV.select :holdingCoil @19:07:46.09 delay 1 second @19:07:47.11 AS.reconfigure ASfast @19:07:47.17 AS.seek 0ml @19:08:14.55 delay 3 seconds @19:08:17.58 AFV.select :carrierSolution @19:08:17.68 delay 2 seconds @19:08:19.70 AS.reconfigure ASconfig @19:08:19.77 AS.seek 1ml @19:09:17.55 delay 1 second @19:09:18.57 AFV.select :holdingCoil @19:09:18.68 delay 1 second @19:09:19.70 AS.reconfigure ASfast @19:09:19.76 AS.seek 0ml @19:09:47.11 delay 3 seconds @19:09:50.13 AFV.select :carrierSolution @19:09:50.23 delay 2 seconds @19:09:52.25 AS.reconfigure ASconfig @19:09:52.32 AS.seek 1ml @19:10:50.11 delay 1 second @19:10:51.13 AFV.select :holdingCoil @19:10:51.23 delay 1 second @19:10:52.25 AS.reconfigure ASfast @19:10:52.31 AS.seek 0ml @19:11:19.69 delay 3 seconds @19:11:22.71 AFV.select :carrierSolution @19:11:22.81 delay 2 seconds @19:11:24.83 AS.reconfigure ASconfig @19:11:24.90 AS.seek 1ml @19:12:22.69 delay 1 second @19:12:23.71 AFV.select :holdingCoil @19:12:23.82 delay 1 second @19:12:24.84 AS.reconfigure ASfast @19:12:24.90 AS.seek 0ml @19:12:52.25 delay 3 seconds @19:12:55.27 AFV.select :carrierSolution @19:12:55.37 delay 2 seconds @19:12:57.39 AS.reconfigure ASconfig @19:12:57.45 AS.seek 1ml @19:13:55.24 delay 1 second @19:13:56.26 AFV.select :holdingCoil @19:13:56.37 delay 1 second @19:13:57.39 AS.reconfigure ASnorm @19:13:57.45 AS.seek 0ml @19:14:48.17
AFV.select :carrierSolution @19:14:48.28 delay 2 seconds @19:14:50.30 AS.reconfigure ASconfig @19:14:50.36 AS.seek 0.5ml @19:15:19.83 delay 1 second @19:15:20.85 AFV.select :holdingCoil @19:15:20.95 ATV.dial :air1 @19:15:22.47 AS.reconfigure ASslow2 @19:15:22.53 AS.seek 0.51ml @19:15:32.74 delay 5 seconds @19:15:37.76 ATV.dial :extSrc @19:15:39.49 AS.seek 0.53ml @19:15:59.91 delay 10 seconds @19:16:09.94 ATV.dial :air1 @19:16:11.66 AS.reconfigure ASslow1 @19:16:11.72 AS.seek 0.56ml @19:16:18.05 ATV.dial :waste @19:16:18.61 AS.reconfigure ASconfig @19:16:18.67 AS.seek :empty @19:16:51.35 Setting up PCR for Ss -- writing /var/log/esp/Ss18dec1319h.pcr @19:16:51.37 Building PCR Slug from extSrc with MM1 mix, PP2 primer @19:16:51.40 Priming with carrier @19:16:51.41 ATV.dial ARV @19:16:52.94 ARV.dial :carrier @19:16:53.30 AS.reconfigure ASnorm @19:16:53.36 AS.seek 0.499ml @19:17:18.95 AFV.select :carrierSolution @19:17:19.07 delay 2 seconds @19:17:21.09 AS.reconfigure ASconfig @19:17:21.16 AS.seek 1ml @19:17:50.25 delay 1 second @19:17:51.27 AFV.select :holdingCoil @19:17:51.37 delay 1 second @19:17:52.39 ATV.dial :waste @19:17:54.08 AS.reconfigure ASnorm @19:17:54.14 AS.seek :empty @19:18:44.88 AFV.select :carrierSolution @19:18:45.00 delay 2 seconds @19:18:47.03 AS.reconfigure ASconfig @19:18:47.09 AS.seek 0.5ml @19:19:16.22 delay 1 second @19:19:17.24 AFV.select :holdingCoil @19:19:17.34 Building Slug @19:19:17.35 ATV.dial :ARV @19:19:19.02 delay 2 seconds @19:19:21.04 ARV.dial :air1 @19:19:21.46 delay 2 seconds @19:19:23.53 AS.reconfigure ASslow2 @19:19:23.59 AS.seek 0.506ml @19:19:29.86 delay 2 seconds @19:19:31.88 ARV.dial :MM1 @19:19:32.50 delay 2 seconds @19:19:34.52 Adding MM1 @19:19:34.60 AS.seek 0.524ml @19:19:53.03 delay 25 seconds @19:20:18.05 ARV.dial :PP2 @19:20:18.46 Adding PP2 @19:20:18.47 delay 2 seconds @19:20:20.55 AS.seek 0.53ml @19:20:26.72 delay 10 seconds @19:20:36.75 ARV.dial :air2 @19:20:37.16 adding ARV air @19:20:37.17 delay 2 seconds @19:20:39.25 AS.seek 0.536ml @19:20:45.58 delay 5 seconds @19:20:50.60 ARV.dial :carrier @19:20:51.91 delay 7 seconds @19:20:58.99 AS.seek 0.594ml @19:21:57.71 delay 5 seconds @19:22:02.73 ATV.dial :extSrc @19:22:03.30 delay 2 seconds @19:22:05.37 AS.seek 0.6ml @19:22:11.69 delay 4 seconds @19:22:15.71 ATV.dial ARV @19:22:16.27 delay 2 seconds @19:22:18.29 ARV.dial :carrier @19:22:18.30 delay 2 seconds @19:22:20.38 AS.seek 0.65ml @19:23:10.93 delay 4 seconds @19:23:14.95 ATV.dial PCR @19:23:15.74 ASV.dial :waste1 @19:23:15.75 ARV.dial :air1 @19:23:16.19 Positioning Slug by 0.324ml @19:23:16.28 AS.seek 0.326ml @19:28:42.18 delay 5 seconds @19:28:47.20 ATV.dial ARV @19:28:47.99 ASV.dial :PCRlock @19:28:48.39 delay 5 seconds @19:28:53.41 Running Ss Profile60C350 thermocycling @19:28:53.42 PCR.program Profile60C350 @19:28:53.57 Profile60C350 should complete in < 1:48:05 @19:28:53.58 PCR.run 2:20:06.25 @20:50:56.26 PCR.off @20:50:58.25 PCR for Ss completed -- closed /var/log/esp/Ss18dec1319h.pcr @20:50:58.32 Cleaning PCR @20:50:58.33 AFV.select :carrierSolution @20:50:58.45 delay 2 seconds @20:51:00.47 AS.reconfigure ASconfig @20:51:00.53 AS.seek 0.5ml @20:51:10.85 delay 1 second @20:51:11.87 AFV.select :holdingCoil @20:51:11.97 ASV.dial :waste1 @20:51:12.37 ATV.dial :air3 @20:51:13.67 AS.reconfigure ASslow1 @20:51:13.74 AS.seek 0.51ml @20:51:16.01 delay 2 seconds @20:51:18.04 ATV.dial :bleach @20:51:18.68 AS.seek 0.81ml @20:52:19.29 delay 3 seconds @20:52:22.31 ATV.dial :air3 @20:52:22.87 delay 3 seconds @20:52:25.94 AS.seek 0.835ml @20:52:31.24 delay 3 seconds @20:52:34.26 ATV.dial :bleach @20:52:34.88 AS.seek 0.82ml @20:52:38.21 delay 3 seconds @20:52:41.23 ARV.dial :waste @20:52:41.87 delay 3 seconds @20:52:44.89 ATV.dial :ARV @20:52:45.89 AS.reconfigure ASconfig @20:52:45.95 AS.seek 0.47ml @20:53:06.36 AFV.select :carrierSolution @20:53:06.48 delay 2 seconds @20:53:08.51 AS.seek 1ml @20:53:39.29 delay 1 second @20:53:40.31 AFV.select :holdingCoil @20:53:40.41 delay 1 second @20:53:41.79 AS.reconfigure ASfast @20:53:41.88 AS.seek 0ml @20:54:09.23 AFV.select :carrierSolution @20:54:09.35 delay 2 seconds @20:54:11.37 AS.reconfigure ASconfig @20:54:11.43 AS.seek 1ml @20:55:09.18 delay 1 second @20:55:10.20 AFV.select :holdingCoil @20:55:10.30 delay 1 second @20:55:11.32 AS.reconfigure ASfast @20:55:11.38 AS.seek 0ml @20:55:38.73 AFV.select :carrierSolution @20:55:38.85 delay 2 seconds @20:55:40.87 AS.reconfigure ASconfig @20:55:40.93 AS.seek 1ml @20:56:38.73 delay 1 second @20:56:39.75 AFV.select :holdingCoil @20:56:39.85 delay 1 second @20:56:40.88 AS.reconfigure ASfast @20:56:40.93 AS.seek 0ml @20:57:08.32 ARV.dial between carrier and air1 @20:57:08.84 ATV.dial :air3 @20:57:10.13 AS.reconfigure ASslow1 @20:57:10.19 AS.seek 0.01ml @20:57:12.51 delay 2 seconds @20:57:14.53 ATV.dial :bleach @20:57:15.16 AS.seek 0.81ml @20:59:56.29 delay 3 seconds @20:59:59.31 ATV.dial :air3 @20:59:59.89 delay 3 seconds @21:00:02.96 AS.seek 0.835ml @21:00:08.29 delay 3 seconds @21:00:11.31 ATV.dial :bleach @21:00:11.95 AS.seek 0.82ml @21:00:15.28 delay 3 seconds @21:00:18.30 ATV.dial PCR @21:00:18.71 AS.reconfigure ASconfig @21:00:18.76 AS.seek :empty @21:01:06.24 PCR.program :bleachProfile @21:01:06.29 PCR program should complete in < 2:45 @21:01:06.30 PCR.run 10:00 @21:04:05.10 PCR.off @21:04:05.13 AFV.select :carrierSolution @21:04:05.25 delay 2 seconds @21:04:07.28 AS.seek 1ml @21:05:05.09 delay 1 second @21:05:06.11 AFV.select :holdingCoil @21:05:06.21 delay 1 second @21:05:07.23 AS.reconfigure ASfast @21:05:07.29 AS.seek 0ml @21:05:34.64 delay 3 seconds @21:05:37.66 AFV.select :carrierSolution @21:05:37.76 delay 2 seconds @21:05:39.78 AS.reconfigure ASconfig @21:05:39.85 AS.seek 1ml @21:06:37.62 delay 1 second @21:06:38.64 AFV.select :holdingCoil @21:06:38.74 delay 1 second @21:06:39.76 AS.reconfigure ASfast @21:06:39.82 AS.seek 0ml @21:07:07.17 delay 3 seconds @21:07:10.19 AFV.select :carrierSolution @21:07:10.29 delay 2 seconds @21:07:12.31 AS.reconfigure ASconfig @21:07:12.38 AS.seek 1ml @21:08:10.18 delay 1 second @21:08:11.20 AFV.select :holdingCoil @21:08:11.30 delay 1 second @21:08:12.32 AS.reconfigure ASfast @21:08:12.38 AS.seek 0ml @21:08:40.14 delay 3 seconds @21:08:43.16 AFV.select :carrierSolution @21:08:43.26 delay 2 seconds @21:08:45.28 AS.reconfigure ASconfig @21:08:45.35 AS.seek 1ml @21:09:43.14 delay 1 second @21:09:44.16 AFV.select :holdingCoil @21:09:44.26 delay 1 second @21:09:45.28 AS.reconfigure ASfast @21:09:45.34 AS.seek 0ml @21:10:12.70 delay 3 seconds @21:10:15.72 AFV.select :carrierSolution @21:10:15.82 delay 2 seconds @21:10:17.84 AS.reconfigure ASconfig @21:10:17.91 AS.seek 1ml @21:11:15.71 delay 1 second @21:11:16.73 AFV.select :holdingCoil @21:11:16.83 delay 1 second @21:11:17.85 AS.reconfigure ASnorm @21:11:17.91 AS.seek 0ml @21:12:08.63
AFV.select :carrierSolution @21:12:08.74 delay 2 seconds @21:12:10.76 AS.reconfigure ASconfig @21:12:10.82 AS.seek 0.5ml @21:12:39.92 delay 1 second @21:12:40.94 AFV.select :holdingCoil @21:12:41.05 ATV.dial :air1 @21:12:42.56 AS.reconfigure ASslow2 @21:12:42.62 AS.seek 0.506ml @21:12:49.23 ATV.dial :extSrc @21:12:50.95 AS.seek 0.512ml @21:12:57.21 delay 5 seconds @21:13:02.23 ATV.dial :air1 @21:13:04.34 AS.reconfigure ASslow1 @21:13:04.40 AS.seek 0.542ml @21:13:10.76 ATV.dial :waste @21:13:11.32 AS.reconfigure ASconfig @21:13:11.38 AS.seek :empty @21:13:43.02 Setting up PCR for Ss -- writing /var/log/esp/Ss18dec1321h.pcr @21:13:43.04 Building PCR Slug from extSrc with MM1 mix, PP2 primer @21:13:43.06 Priming with carrier @21:13:43.08 ATV.dial ARV @21:13:44.59 ARV.dial :carrier @21:13:44.95 AS.reconfigure ASnorm @21:13:45.01 AS.seek 0.499ml @21:14:10.56 AFV.select :carrierSolution @21:14:10.69 delay 2 seconds @21:14:12.72 AS.reconfigure ASconfig @21:14:12.78 AS.seek 1ml @21:14:41.86 delay 1 second @21:14:42.88 AFV.select :holdingCoil @21:14:42.98 delay 1 second @21:14:44.00 ATV.dial :waste @21:14:45.68 AS.reconfigure ASnorm @21:14:45.74 AS.seek :empty @21:15:36.50 AFV.select :carrierSolution @21:15:36.63 delay 2 seconds @21:15:38.65 AS.reconfigure ASconfig @21:15:38.71 AS.seek 0.5ml @21:16:07.84 delay 1 second @21:16:08.86 AFV.select :holdingCoil @21:16:08.96 Building Slug @21:16:08.97 ATV.dial :ARV @21:16:10.64 delay 2 seconds @21:16:12.66 ARV.dial :air1 @21:16:13.08 delay 2 seconds @21:16:15.15 AS.reconfigure ASslow2 @21:16:15.22 AS.seek 0.506ml @21:16:21.64 delay 2 seconds @21:16:23.66 ARV.dial :MM1 @21:16:24.29 delay 2 seconds @21:16:26.31 Adding MM1 @21:16:26.39 AS.seek 0.524ml @21:16:44.75 delay 25 seconds @21:17:09.77 ARV.dial :PP2 @21:17:10.17 Adding PP2 @21:17:10.18 delay 2 seconds @21:17:12.26 AS.seek 0.53ml @21:17:18.45 delay 10 seconds @21:17:28.48 ARV.dial :air2 @21:17:28.89 adding ARV air @21:17:28.90 delay 2 seconds @21:17:30.98 AS.seek 0.536ml @21:17:37.39 delay 5 seconds @21:17:42.41 ARV.dial :carrier @21:17:43.73 delay 7 seconds @21:17:50.81 AS.seek 0.594ml @21:18:49.53 delay 5 seconds @21:18:54.55 ATV.dial :extSrc @21:18:55.12 delay 2 seconds @21:18:57.19 AS.seek 0.6ml @21:19:03.48 delay 4 seconds @21:19:07.50 ATV.dial ARV @21:19:08.06 delay 2 seconds @21:19:10.08 ARV.dial :carrier @21:19:10.09 delay 2 seconds @21:19:12.17 AS.seek 0.65ml @21:20:02.62 delay 4 seconds @21:20:06.64 ATV.dial PCR @21:20:07.42 ASV.dial :waste1 @21:20:07.43 ARV.dial :air1 @21:20:07.87 Positioning Slug by 0.324ml @21:20:07.96 AS.seek 0.326ml @21:25:34.29 delay 5 seconds @21:25:39.31 ATV.dial ARV @21:25:40.09 ASV.dial :PCRlock @21:25:40.50 delay 5 seconds @21:25:45.53 Running Ss Profile60C350 thermocycling @21:25:45.54 PCR.program Profile60C350 @21:25:45.70 Profile60C350 should complete in < 1:48:05 @21:25:45.71 PCR.run 2:20:06.25 @22:47:55.34 PCR.off @22:47:57.34 PCR for Ss completed -- closed /var/log/esp/Ss18dec1321h.pcr @22:47:57.42 Cleaning PCR @22:47:57.43 AFV.select :carrierSolution @22:47:57.54 delay 2 seconds @22:47:59.56 AS.reconfigure ASconfig @22:47:59.62 AS.seek 0.5ml @22:48:09.97 delay 1 second @22:48:10.99 AFV.select :holdingCoil @22:48:11.10 ASV.dial :waste1 @22:48:11.50 ATV.dial :air3 @22:48:12.80 AS.reconfigure ASslow1 @22:48:12.87 AS.seek 0.51ml @22:48:15.15 delay 2 seconds @22:48:17.17 ATV.dial :bleach @22:48:17.81 AS.seek 0.81ml @22:49:18.42 delay 3 seconds @22:49:21.44 ATV.dial :air3 @22:49:22.00 delay 3 seconds @22:49:25.07 AS.seek 0.835ml @22:49:30.38 delay 3 seconds @22:49:33.40 ATV.dial :bleach @22:49:34.02 AS.seek 0.82ml @22:49:37.32 delay 3 seconds @22:49:40.34 ARV.dial :waste @22:49:40.97 delay 3 seconds @22:49:43.99 ATV.dial :ARV @22:49:44.99 AS.reconfigure ASconfig @22:49:45.05 AS.seek 0.47ml @22:50:05.46 AFV.select :carrierSolution @22:50:05.58 delay 2 seconds @22:50:07.61 AS.seek 1ml @22:50:38.38 delay 1 second @22:50:39.40 AFV.select :holdingCoil @22:50:39.50 delay 1 second @22:50:40.52 AS.reconfigure ASfast @22:50:40.58 AS.seek 0ml @22:51:07.93 AFV.select :carrierSolution @22:51:08.05 delay 2 seconds @22:51:10.07 AS.reconfigure ASconfig @22:51:10.13 AS.seek 1ml @22:52:07.89 delay 1 second @22:52:08.91 AFV.select :holdingCoil @22:52:09.01 delay 1 second @22:52:10.03 AS.reconfigure ASfast @22:52:10.09 AS.seek 0ml @22:52:37.54 AFV.select :carrierSolution @22:52:37.66 delay 2 seconds @22:52:39.68 AS.reconfigure ASconfig @22:52:39.74 AS.seek 1ml @22:53:37.91 delay 1 second @22:53:38.93 AFV.select :holdingCoil @22:53:39.03 delay 1 second @22:53:40.05 AS.reconfigure ASfast @22:53:40.11 AS.seek 0ml @22:54:07.49 ARV.dial between carrier and air1 @22:54:08.00 ATV.dial :air3 @22:54:09.30 AS.reconfigure ASslow1 @22:54:09.36 AS.seek 0.01ml @22:54:11.69 delay 2 seconds @22:54:13.71 ATV.dial :bleach @22:54:14.34 AS.seek 0.81ml @22:56:55.52 delay 3 seconds @22:56:58.54 ATV.dial :air3 @22:56:59.11 delay 3 seconds @22:57:02.18 AS.seek 0.835ml @22:57:07.52 delay 3 seconds @22:57:10.54 ATV.dial :bleach @22:57:11.17 AS.seek 0.82ml @22:57:14.47 delay 3 seconds @22:57:17.49 ATV.dial PCR @22:57:17.89 AS.reconfigure ASconfig @22:57:17.95 AS.seek :empty @22:58:05.42 PCR.program :bleachProfile @22:58:05.47 PCR program should complete in < 2:45 @22:58:05.48 PCR.run 10:00 @23:01:04.29 PCR.off @23:01:04.32 AFV.select :carrierSolution @23:01:04.44 delay 2 seconds @23:01:06.47 AS.seek 1ml @23:02:04.25 delay 1 second @23:02:05.27 AFV.select :holdingCoil @23:02:05.37 delay 1 second @23:02:06.39 AS.reconfigure ASfast @23:02:06.45 AS.seek 0ml @23:02:33.94 delay 3 seconds @23:02:36.96 AFV.select :carrierSolution @23:02:37.06 delay 2 seconds @23:02:39.08 AS.reconfigure ASconfig @23:02:39.15 AS.seek 1ml @23:03:36.91 delay 1 second @23:03:37.93 AFV.select :holdingCoil @23:03:38.03 delay 1 second @23:03:39.05 AS.reconfigure ASfast @23:03:39.11 AS.seek 0ml @23:04:06.46 delay 3 seconds @23:04:09.48 AFV.select :carrierSolution @23:04:09.58 delay 2 seconds @23:04:11.60 AS.reconfigure ASconfig @23:04:11.67 AS.seek 1ml @23:05:09.43 delay 1 second @23:05:10.45 AFV.select :holdingCoil @23:05:10.55 delay 1 second @23:05:11.57 AS.reconfigure ASfast @23:05:11.63 AS.seek 0ml @23:05:38.97 delay 3 seconds @23:05:41.99 AFV.select :carrierSolution @23:05:42.09 delay 2 seconds @23:05:44.11 AS.reconfigure ASconfig @23:05:44.18 AS.seek 1ml @23:06:41.99 delay 1 second @23:06:43.01 AFV.select :holdingCoil @23:06:43.11 delay 1 second @23:06:44.13 AS.reconfigure ASfast @23:06:44.19 AS.seek 0ml @23:07:11.55 delay 3 seconds @23:07:14.57 AFV.select :carrierSolution @23:07:14.67 delay 2 seconds @23:07:16.69 AS.reconfigure ASconfig @23:07:16.76 AS.seek 1ml @23:08:14.55 delay 1 second @23:08:15.57 AFV.select :holdingCoil @23:08:15.69 delay 1 second @23:08:16.71 AS.reconfigure ASnorm @23:08:16.77 AS.seek 0ml @23:09:07.50
AFV.select :carrierSolution @23:09:07.62 delay 2 seconds @23:09:09.64 AS.reconfigure ASconfig @23:09:09.70 AS.seek 0.5ml @23:09:38.77 delay 1 second @23:09:39.79 AFV.select :holdingCoil @23:09:39.89 ATV.dial :air1 @23:09:41.41 AS.reconfigure ASslow1 @23:09:41.47 AS.seek 0.52ml @23:09:45.77 delay 5 seconds @23:09:50.79 ATV.dial :extSrc @23:09:52.51 AS.reconfigure ASslow2 @23:09:52.57 AS.seek 0.545ml @23:10:17.86 delay 10 seconds @23:10:27.88 ATV.dial :air1 @23:10:29.59 AS.reconfigure ASslow1 @23:10:29.65 AS.seek 0.575ml @23:10:35.96 ATV.dial :waste @23:10:36.52 AS.reconfigure ASconfig @23:10:36.58 AS.seek :empty @23:11:10.14 Setting up PCR for Ss -- writing /var/log/esp/Ss18dec1323h.pcr @23:11:10.17 Building PCR Slug from extSrc with MM1 mix, PP2 primer @23:11:10.19 Priming with carrier @23:11:10.21 ATV.dial ARV @23:11:11.72 ARV.dial :carrier @23:11:12.45 AS.reconfigure ASnorm @23:11:12.51 AS.seek 0.499ml @23:11:38.07 AFV.select :carrierSolution @23:11:38.20 delay 2 seconds @23:11:40.23 AS.reconfigure ASconfig @23:11:40.29 AS.seek 1ml @23:12:09.37 delay 1 second @23:12:10.39 AFV.select :holdingCoil @23:12:10.49 delay 1 second @23:12:11.51 ATV.dial :waste @23:12:13.19 AS.reconfigure ASnorm @23:12:13.25 AS.seek :empty @23:13:03.99 AFV.select :carrierSolution @23:13:04.11 delay 2 seconds @23:13:06.14 AS.reconfigure ASconfig @23:13:06.20 AS.seek 0.5ml @23:13:35.32 delay 1 second @23:13:36.34 AFV.select :holdingCoil @23:13:36.44 Building Slug @23:13:36.45 ATV.dial :ARV @23:13:38.13 delay 2 seconds @23:13:40.15 ARV.dial :air1 @23:13:40.55 delay 2 seconds @23:13:42.62 AS.reconfigure ASslow2 @23:13:42.68 AS.seek 0.506ml @23:13:49.00 delay 2 seconds @23:13:51.02 ARV.dial :MM1 @23:13:51.65 delay 2 seconds @23:13:53.67 Adding MM1 @23:13:53.75 AS.seek 0.524ml @23:14:12.21 delay 25 seconds @23:14:37.23 ARV.dial :PP2 @23:14:37.63 Adding PP2 @23:14:37.64 delay 2 seconds @23:14:39.72 AS.seek 0.53ml @23:14:45.91 delay 10 seconds @23:14:55.94 ARV.dial :air2 @23:14:56.35 adding ARV air @23:14:56.36 delay 2 seconds @23:14:58.44 AS.seek 0.536ml @23:15:04.94 delay 5 seconds @23:15:09.96 ARV.dial :carrier @23:15:11.27 delay 7 seconds @23:15:18.35 AS.seek 0.594ml @23:16:16.99 delay 5 seconds @23:16:22.01 ATV.dial :extSrc @23:16:22.58 delay 2 seconds @23:16:24.65 AS.seek 0.6ml @23:16:30.97 delay 4 seconds @23:16:34.99 ATV.dial ARV @23:16:35.55 delay 2 seconds @23:16:37.57 ARV.dial :carrier @23:16:37.58 delay 2 seconds @23:16:39.66 AS.seek 0.65ml @23:17:30.24 delay 4 seconds @23:17:34.26 ATV.dial PCR @23:17:35.05 ASV.dial :waste1 @23:17:35.06 ARV.dial :air1 @23:17:35.49 Positioning Slug by 0.324ml @23:17:35.58 AS.seek 0.326ml @23:23:01.71 delay 5 seconds @23:23:06.73 ATV.dial ARV @23:23:07.52 ASV.dial :PCRlock @23:23:07.93 delay 5 seconds @23:23:12.95 Running Ss Profile60C350 thermocycling @23:23:12.96 PCR.program Profile60C350 @23:23:13.12 Profile60C350 should complete in < 1:48:05 @23:23:13.13 PCR.run 2:20:06.25 @00:45:07.66CET14-Dec-18 PCR.off @00:45:09.65 PCR for Ss completed -- closed /var/log/esp/Ss18dec1323h.pcr @00:45:09.73 Cleaning PCR @00:45:09.74 AFV.select :carrierSolution @00:45:09.86 delay 2 seconds @00:45:11.88 AS.reconfigure ASconfig @00:45:11.94 AS.seek 0.5ml @00:45:22.25 delay 1 second @00:45:23.27 AFV.select :holdingCoil @00:45:23.37 ASV.dial :waste1 @00:45:23.78 ATV.dial :air3 @00:45:25.08 AS.reconfigure ASslow1 @00:45:25.15 AS.seek 0.51ml @00:45:27.43 delay 2 seconds @00:45:29.45 ATV.dial :bleach @00:45:30.09 AS.seek 0.81ml @00:46:30.71 delay 3 seconds @00:46:33.73 ATV.dial :air3 @00:46:34.29 delay 3 seconds @00:46:37.36 AS.seek 0.835ml @00:46:42.67 delay 3 seconds @00:46:45.69 ATV.dial :bleach @00:46:46.31 AS.seek 0.82ml @00:46:49.62 delay 3 seconds @00:46:52.64 ARV.dial :waste @00:46:53.26 delay 3 seconds @00:46:56.28 ATV.dial :ARV @00:46:57.28 AS.reconfigure ASconfig @00:46:57.35 AS.seek 0.47ml @00:47:17.77 AFV.select :carrierSolution @00:47:17.89 delay 2 seconds @00:47:19.92 AS.seek 1ml @00:47:50.76 delay 1 second @00:47:51.78 AFV.select :holdingCoil @00:47:51.88 delay 1 second @00:47:52.90 AS.reconfigure ASfast @00:47:52.96 AS.seek 0ml @00:48:20.34 AFV.select :carrierSolution @00:48:20.44 delay 2 seconds @00:48:22.46 AS.reconfigure ASconfig @00:48:22.52 AS.seek 1ml @00:49:20.31 delay 1 second @00:49:21.33 AFV.select :holdingCoil @00:49:21.43 delay 1 second @00:49:22.45 AS.reconfigure ASfast @00:49:22.51 AS.seek 0ml @00:49:50.22 AFV.select :carrierSolution @00:49:50.34 delay 2 seconds @00:49:52.36 AS.reconfigure ASconfig @00:49:52.42 AS.seek 1ml @00:50:50.18 delay 1 second @00:50:51.20 AFV.select :holdingCoil @00:50:51.30 delay 1 second @00:50:52.32 AS.reconfigure ASfast @00:50:52.38 AS.seek 0ml @00:51:19.75 ARV.dial between carrier and air1 @00:51:20.26 ATV.dial :air3 @00:51:21.55 AS.reconfigure ASslow1 @00:51:21.61 AS.seek 0.01ml @00:51:23.98 delay 2 seconds @00:51:26.00 ATV.dial :bleach @00:51:27.01 AS.seek 0.81ml @00:54:08.17 delay 3 seconds @00:54:11.19 ATV.dial :air3 @00:54:11.76 delay 3 seconds @00:54:14.83 AS.seek 0.835ml @00:54:20.16 delay 3 seconds @00:54:23.18 ATV.dial :bleach @00:54:23.81 AS.seek 0.82ml @00:54:27.07 delay 3 seconds @00:54:30.09 ATV.dial PCR @00:54:30.50 AS.reconfigure ASconfig @00:54:30.56 AS.seek :empty @00:55:18.01 PCR.program :bleachProfile @00:55:18.06 PCR program should complete in < 2:45 @00:55:18.08 PCR.run 10:00 @00:58:16.88 PCR.off @00:58:16.91 AFV.select :carrierSolution @00:58:17.03 delay 2 seconds @00:58:19.06 AS.seek 1ml @00:59:16.90 delay 1 second @00:59:17.92 AFV.select :holdingCoil @00:59:18.03 delay 1 second @00:59:19.05 AS.reconfigure ASfast @00:59:19.11 AS.seek 0ml @00:59:46.59 delay 3 seconds @00:59:49.61 AFV.select :carrierSolution @00:59:49.71 delay 2 seconds @00:59:51.73 AS.reconfigure ASconfig @00:59:51.80 AS.seek 1ml @01:00:49.57 delay 1 second @01:00:50.59 AFV.select :holdingCoil @01:00:50.69 delay 1 second @01:00:51.71 AS.reconfigure ASfast @01:00:51.77 AS.seek 0ml @01:01:19.15 delay 3 seconds @01:01:22.17 AFV.select :carrierSolution @01:01:22.27 delay 2 seconds @01:01:24.29 AS.reconfigure ASconfig @01:01:24.36 AS.seek 1ml @01:02:22.14 delay 1 second @01:02:23.16 AFV.select :holdingCoil @01:02:23.26 delay 1 second @01:02:24.28 AS.reconfigure ASfast @01:02:24.34 AS.seek 0ml @01:02:51.68 delay 3 seconds @01:02:54.70 AFV.select :carrierSolution @01:02:54.80 delay 2 seconds @01:02:56.82 AS.reconfigure ASconfig @01:02:56.89 AS.seek 1ml @01:03:54.68 delay 1 second @01:03:55.70 AFV.select :holdingCoil @01:03:55.80 delay 1 second @01:03:56.82 AS.reconfigure ASfast @01:03:56.88 AS.seek 0ml @01:04:24.31 delay 3 seconds @01:04:27.33 AFV.select :carrierSolution @01:04:27.43 delay 2 seconds @01:04:29.45 AS.reconfigure ASconfig @01:04:29.51 AS.seek 1ml @01:05:27.26 delay 1 second @01:05:28.28 AFV.select :holdingCoil @01:05:28.38 delay 1 second @01:05:29.40 AS.reconfigure ASnorm @01:05:29.46 AS.seek 0ml @01:06:20.18
AFV.select :carrierSolution @01:06:20.29 delay 2 seconds @01:06:22.31 AS.reconfigure ASconfig @01:06:22.37 AS.seek 0.5ml @01:06:51.45 delay 1 second @01:06:52.47 AFV.select :holdingCoil @01:06:52.57 ATV.dial :air1 @01:06:54.09 AS.reconfigure ASslow2 @01:06:54.14 AS.seek 0.506ml @01:07:00.39 ATV.dial :extSrc @01:07:02.09 AS.seek 0.512ml @01:07:08.33 delay 5 seconds @01:07:13.35 ATV.dial :air1 @01:07:15.06 AS.reconfigure ASslow1 @01:07:15.12 AS.seek 0.542ml @01:07:21.43 ATV.dial :waste @01:07:22.00 AS.reconfigure ASconfig @01:07:22.06 AS.seek :empty @01:07:53.72 Setting up PCR for Ss -- writing /var/log/esp/Ss18dec1401h.pcr @01:07:53.74 Building PCR Slug from extSrc with MM1 mix, PP2 primer @01:07:53.77 Priming with carrier @01:07:53.79 ATV.dial ARV @01:07:55.29 ARV.dial :carrier @01:07:55.65 AS.reconfigure ASnorm @01:07:55.71 AS.seek 0.499ml @01:08:21.27 AFV.select :carrierSolution @01:08:21.39 delay 2 seconds @01:08:23.41 AS.reconfigure ASconfig @01:08:23.48 AS.seek 1ml @01:08:52.56 delay 1 second @01:08:53.58 AFV.select :holdingCoil @01:08:53.68 delay 1 second @01:08:54.70 ATV.dial :waste @01:08:56.39 AS.reconfigure ASnorm @01:08:56.45 AS.seek :empty @01:09:47.57 AFV.select :carrierSolution @01:09:47.70 delay 2 seconds @01:09:49.72 AS.reconfigure ASconfig @01:09:49.78 AS.seek 0.5ml @01:10:18.90 delay 1 second @01:10:19.92 AFV.select :holdingCoil @01:10:20.02 Building Slug @01:10:20.03 ATV.dial :ARV @01:10:21.70 delay 2 seconds @01:10:23.72 ARV.dial :air1 @01:10:24.13 delay 2 seconds @01:10:26.20 AS.reconfigure ASslow2 @01:10:26.26 AS.seek 0.506ml @01:10:32.58 delay 2 seconds @01:10:34.60 ARV.dial :MM1 @01:10:35.22 delay 2 seconds @01:10:37.24 Adding MM1 @01:10:37.32 AS.seek 0.524ml @01:10:55.89 delay 25 seconds @01:11:20.91 ARV.dial :PP2 @01:11:21.31 Adding PP2 @01:11:21.32 delay 2 seconds @01:11:23.40 AS.seek 0.53ml @01:11:29.93 delay 10 seconds @01:11:39.96 ARV.dial :air2 @01:11:40.36 adding ARV air @01:11:40.37 delay 2 seconds @01:11:42.45 AS.seek 0.536ml @01:11:48.76 delay 5 seconds @01:11:53.78 ARV.dial :carrier @01:11:55.08 delay 7 seconds @01:12:02.16 AS.seek 0.594ml @01:13:00.83 delay 5 seconds @01:13:05.85 ATV.dial :extSrc @01:13:06.42 delay 2 seconds @01:13:08.49 AS.seek 0.6ml @01:13:14.75 delay 4 seconds @01:13:18.77 ATV.dial ARV @01:13:19.34 delay 2 seconds @01:13:21.36 ARV.dial :carrier @01:13:21.37 delay 2 seconds @01:13:23.45 AS.seek 0.65ml @01:14:13.97 delay 4 seconds @01:14:17.99 ATV.dial PCR @01:14:18.76 ASV.dial :waste1 @01:14:18.77 ARV.dial :air1 @01:14:19.20 Positioning Slug by 0.324ml @01:14:19.30 AS.seek 0.326ml @01:19:45.22 delay 5 seconds @01:19:50.24 ATV.dial ARV @01:19:51.03 ASV.dial :PCRlock @01:19:51.44 delay 5 seconds @01:19:56.46 Running Ss Profile60C350 thermocycling @01:19:56.47 PCR.program Profile60C350 @01:19:56.62 Profile60C350 should complete in < 1:48:05 @01:19:56.63 PCR.run 2:20:06.25 @02:41:51.19 PCR.off @02:41:53.20 PCR for Ss completed -- closed /var/log/esp/Ss18dec1401h.pcr @02:41:53.27 Cleaning PCR @02:41:53.28 AFV.select :carrierSolution @02:41:53.40 delay 2 seconds @02:41:55.42 AS.reconfigure ASconfig @02:41:55.48 AS.seek 0.5ml @02:42:05.80 delay 1 second @02:42:06.82 AFV.select :holdingCoil @02:42:06.92 ASV.dial :waste1 @02:42:07.33 ATV.dial :air3 @02:42:08.63 AS.reconfigure ASslow1 @02:42:08.70 AS.seek 0.51ml @02:42:11.01 delay 2 seconds @02:42:13.03 ATV.dial :bleach @02:42:13.66 AS.seek 0.81ml @02:43:14.32 delay 3 seconds @02:43:17.34 ATV.dial :air3 @02:43:17.91 delay 3 seconds @02:43:20.98 AS.seek 0.835ml @02:43:26.30 delay 3 seconds @02:43:29.32 ATV.dial :bleach @02:43:29.94 AS.seek 0.82ml @02:43:33.24 delay 3 seconds @02:43:36.26 ARV.dial :waste @02:43:36.90 delay 3 seconds @02:43:39.92 ATV.dial :ARV @02:43:40.82 AS.reconfigure ASconfig @02:43:40.88 AS.seek 0.47ml @02:44:01.29 AFV.select :carrierSolution @02:44:01.41 delay 2 seconds @02:44:03.44 AS.seek 1ml @02:44:34.24 delay 1 second @02:44:35.26 AFV.select :holdingCoil @02:44:35.36 delay 1 second @02:44:36.38 AS.reconfigure ASfast @02:44:36.44 AS.seek 0ml @02:45:03.87 AFV.select :carrierSolution @02:45:03.99 delay 2 seconds @02:45:06.01 AS.reconfigure ASconfig @02:45:06.07 AS.seek 1ml @02:46:03.83 delay 1 second @02:46:04.85 AFV.select :holdingCoil @02:46:04.95 delay 1 second @02:46:05.97 AS.reconfigure ASfast @02:46:06.03 AS.seek 0ml @02:46:33.45 AFV.select :carrierSolution @02:46:33.57 delay 2 seconds @02:46:35.59 AS.reconfigure ASconfig @02:46:35.65 AS.seek 1ml @02:47:33.40 delay 1 second @02:47:34.42 AFV.select :holdingCoil @02:47:34.52 delay 1 second @02:47:35.54 AS.reconfigure ASfast @02:47:35.60 AS.seek 0ml @02:48:02.97 ARV.dial between carrier and air1 @02:48:03.49 ATV.dial :air3 @02:48:04.79 AS.reconfigure ASslow1 @02:48:04.85 AS.seek 0.01ml @02:48:07.22 delay 2 seconds @02:48:09.24 ATV.dial :bleach @02:48:09.88 AS.seek 0.81ml @02:50:51.02 delay 3 seconds @02:50:54.04 ATV.dial :air3 @02:50:54.60 delay 3 seconds @02:50:57.67 AS.seek 0.835ml @02:51:03.00 delay 3 seconds @02:51:06.02 ATV.dial :bleach @02:51:06.64 AS.seek 0.82ml @02:51:09.91 delay 3 seconds @02:51:13.25 ATV.dial PCR @02:51:13.71 AS.reconfigure ASconfig @02:51:13.77 AS.seek :empty @02:52:01.24 PCR.program :bleachProfile @02:52:01.30 PCR program should complete in < 2:45 @02:52:01.31 PCR.run 10:00 @02:55:00.13 PCR.off @02:55:00.16 AFV.select :carrierSolution @02:55:00.28 delay 2 seconds @02:55:02.31 AS.seek 1ml @02:56:00.15 delay 1 second @02:56:01.17 AFV.select :holdingCoil @02:56:01.27 delay 1 second @02:56:02.29 AS.reconfigure ASfast @02:56:02.35 AS.seek 0ml @02:56:29.74 delay 3 seconds @02:56:32.76 AFV.select :carrierSolution @02:56:32.86 delay 2 seconds @02:56:34.88 AS.reconfigure ASconfig @02:56:34.95 AS.seek 1ml @02:57:32.78 delay 1 second @02:57:33.83 AFV.select :holdingCoil @02:57:33.93 delay 1 second @02:57:34.97 AS.reconfigure ASfast @02:57:35.11 AS.seek 0ml @02:58:02.54 delay 3 seconds @02:58:05.56 AFV.select :carrierSolution @02:58:05.66 delay 2 seconds @02:58:07.68 AS.reconfigure ASconfig @02:58:07.75 AS.seek 1ml @02:59:05.57 delay 1 second @02:59:06.59 AFV.select :holdingCoil @02:59:06.69 delay 1 second @02:59:07.71 AS.reconfigure ASfast @02:59:07.77 AS.seek 0ml @02:59:35.13 delay 3 seconds @02:59:38.15 AFV.select :carrierSolution @02:59:38.25 delay 2 seconds @02:59:40.28 AS.reconfigure ASconfig @02:59:40.34 AS.seek 1ml @03:00:38.13 delay 1 second @03:00:39.15 AFV.select :holdingCoil @03:00:39.25 delay 1 second @03:00:40.27 AS.reconfigure ASfast @03:00:40.33 AS.seek 0ml @03:01:07.68 delay 3 seconds @03:01:10.70 AFV.select :carrierSolution @03:01:10.80 delay 2 seconds @03:01:12.82 AS.reconfigure ASconfig @03:01:12.89 AS.seek 1ml @03:02:10.71 delay 1 second @03:02:11.73 AFV.select :holdingCoil @03:02:11.83 delay 1 second @03:02:12.85 AS.reconfigure ASnorm @03:02:12.91 AS.seek 0ml @03:03:03.64
AFV.select :carrierSolution @03:03:03.75 delay 2 seconds @03:03:05.77 AS.reconfigure ASconfig @03:03:05.83 AS.seek 0.5ml @03:03:34.88 delay 1 second @03:03:35.90 AFV.select :holdingCoil @03:03:36.00 ATV.dial :air1 @03:03:37.52 AS.reconfigure ASslow1 @03:03:37.57 AS.seek 0.52ml @03:03:41.87 delay 5 seconds @03:03:46.89 ATV.dial :extSrc @03:03:48.60 AS.reconfigure ASslow2 @03:03:48.66 AS.seek 0.538ml @03:04:06.94 delay 10 seconds @03:04:16.97 ATV.dial :air1 @03:04:18.68 AS.reconfigure ASslow1 @03:04:18.74 AS.seek 0.568ml @03:04:25.08 ATV.dial :waste @03:04:25.65 AS.reconfigure ASconfig @03:04:25.70 AS.seek :empty @03:04:58.86 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118dec1403h.pcr @03:04:58.88 Building PCR Slug from extSrc with MM2 mix, PP4 primer @03:04:58.91 Priming with carrier @03:04:58.93 ATV.dial ARV @03:05:00.44 ARV.dial :carrier @03:05:00.80 AS.reconfigure ASnorm @03:05:00.86 AS.seek 0.499ml @03:05:26.39 AFV.select :carrierSolution @03:05:26.51 delay 2 seconds @03:05:28.54 AS.reconfigure ASconfig @03:05:28.60 AS.seek 1ml @03:05:57.69 delay 1 second @03:05:58.71 AFV.select :holdingCoil @03:05:58.82 delay 1 second @03:05:59.84 ATV.dial :waste @03:06:01.52 AS.reconfigure ASnorm @03:06:01.58 AS.seek :empty @03:06:52.34 AFV.select :carrierSolution @03:06:52.46 delay 2 seconds @03:06:54.48 AS.reconfigure ASconfig @03:06:54.54 AS.seek 0.5ml @03:07:23.68 delay 1 second @03:07:24.70 AFV.select :holdingCoil @03:07:24.80 Building Slug @03:07:24.81 ATV.dial :ARV @03:07:26.48 delay 2 seconds @03:07:28.50 ARV.dial :air1 @03:07:28.92 delay 2 seconds @03:07:30.99 AS.reconfigure ASslow2 @03:07:31.43 AS.seek 0.506ml @03:07:37.82 delay 2 seconds @03:07:39.84 ARV.dial :MM2 @03:07:41.37 delay 2 seconds @03:07:43.39 Adding MM2 @03:07:43.47 AS.seek 0.524ml @03:08:01.85 delay 25 seconds @03:08:26.87 ARV.dial :PP4 @03:08:27.29 Adding PP4 @03:08:27.30 delay 2 seconds @03:08:29.38 AS.seek 0.53ml @03:08:35.69 delay 10 seconds @03:08:45.72 ARV.dial :air3 @03:08:46.12 adding ARV air @03:08:46.13 delay 2 seconds @03:08:48.21 AS.seek 0.536ml @03:08:54.65 delay 5 seconds @03:08:59.67 ARV.dial :carrier @03:09:00.98 delay 7 seconds @03:09:08.06 AS.seek 0.594ml @03:10:06.66 delay 5 seconds @03:10:11.68 ATV.dial :extSrc @03:10:12.24 delay 2 seconds @03:10:14.31 AS.seek 0.6ml @03:10:20.80 delay 4 seconds @03:10:24.82 ATV.dial ARV @03:10:25.38 delay 2 seconds @03:10:27.40 ARV.dial :carrier @03:10:27.41 delay 2 seconds @03:10:29.49 AS.seek 0.65ml @03:11:13.38 Session Ended @03:11:20.12 delay 4 seconds @03:11:24.14 ATV.dial PCR @03:11:24.91 ASV.dial :waste1 @03:11:24.92 ARV.dial :air1 @03:11:25.33 Positioning Slug by 0.324ml @03:11:25.40 AS.seek 0.326ml @03:16:51.48 delay 5 seconds @03:16:56.50 ATV.dial ARV @03:16:57.27 ASV.dial :PCRlock @03:16:57.66 delay 5 seconds @03:17:02.68 Running Tt1 Profile60C300 thermocycling @03:17:02.69 PCR.program Profile60C300 @03:17:02.81 Profile60C300 should complete in < 1:45:21 @03:17:02.82 PCR.run 2:16:41.25 @04:38:34.49 PCR.off @04:38:36.34 PCR for Tt1 completed -- closed /var/log/esp/Tt118dec1403h.pcr @04:38:36.40 Cleaning PCR @04:38:36.41 AFV.select :carrierSolution @04:38:36.51 delay 2 seconds @04:38:38.53 AS.reconfigure ASconfig @04:38:38.58 AS.seek 0.5ml @04:38:48.87 delay 1 second @04:38:49.89 AFV.select :holdingCoil @04:38:49.99 ASV.dial :waste1 @04:38:50.39 ATV.dial :air3 @04:38:51.68 AS.reconfigure ASslow1 @04:38:51.72 AS.seek 0.51ml @04:38:54.01 delay 2 seconds @04:38:56.03 ATV.dial :bleach @04:38:56.65 AS.seek 0.81ml @04:39:57.23 delay 3 seconds @04:40:00.25 ATV.dial :air3 @04:40:00.79 delay 3 seconds @04:40:03.86 AS.seek 0.835ml @04:40:09.16 delay 3 seconds @04:40:12.18 ATV.dial :bleach @04:40:12.79 AS.seek 0.82ml @04:40:16.11 delay 3 seconds @04:40:19.13 ARV.dial :waste @04:40:19.73 delay 3 seconds @04:40:22.75 ATV.dial :ARV @04:40:23.64 AS.reconfigure ASconfig @04:40:23.69 AS.seek 0.47ml @04:40:44.08 AFV.select :carrierSolution @04:40:44.19 delay 2 seconds @04:40:46.22 AS.seek 1ml @04:41:16.97 delay 1 second @04:41:17.99 AFV.select :holdingCoil @04:41:18.09 delay 1 second @04:41:19.11 AS.reconfigure ASfast @04:41:19.16 AS.seek 0ml @04:41:46.49 AFV.select :carrierSolution @04:41:46.60 delay 2 seconds @04:41:48.62 AS.reconfigure ASconfig @04:41:48.67 AS.seek 1ml @04:42:46.45 delay 1 second @04:42:47.47 AFV.select :holdingCoil @04:42:47.57 delay 1 second @04:42:48.59 AS.reconfigure ASfast @04:42:48.64 AS.seek 0ml @04:43:15.96 AFV.select :carrierSolution @04:43:16.07 delay 2 seconds @04:43:18.09 AS.reconfigure ASconfig @04:43:18.14 AS.seek 1ml @04:44:15.89 delay 1 second @04:44:16.91 AFV.select :holdingCoil @04:44:17.01 delay 1 second @04:44:18.03 AS.reconfigure ASfast @04:44:18.08 AS.seek 0ml @04:44:45.54 ARV.dial between carrier and air1 @04:44:46.04 ATV.dial :air3 @04:44:47.33 AS.reconfigure ASslow1 @04:44:47.38 AS.seek 0.01ml @04:44:49.69 delay 2 seconds @04:44:51.71 ATV.dial :bleach @04:44:52.34 AS.seek 0.81ml @04:47:33.50 delay 3 seconds @04:47:36.52 ATV.dial :air3 @04:47:37.08 delay 3 seconds @04:47:40.15 AS.seek 0.835ml @04:47:45.44 delay 3 seconds @04:47:48.46 ATV.dial :bleach @04:47:49.07 AS.seek 0.82ml @04:47:52.36 delay 3 seconds @04:47:55.38 ATV.dial PCR @04:47:55.76 AS.reconfigure ASconfig @04:47:55.80 AS.seek :empty @04:48:43.28 PCR.program :bleachProfile @04:48:43.32 PCR program should complete in < 2:45 @04:48:43.33 PCR.run 10:00 @04:51:42.13 PCR.off @04:51:42.16 AFV.select :carrierSolution @04:51:42.26 delay 2 seconds @04:51:44.29 AS.seek 1ml @04:52:42.08 delay 1 second @04:52:43.10 AFV.select :holdingCoil @04:52:43.20 delay 1 second @04:52:44.22 AS.reconfigure ASfast @04:52:44.27 AS.seek 0ml @04:53:11.72 delay 3 seconds @04:53:14.74 AFV.select :carrierSolution @04:53:14.84 delay 2 seconds @04:53:17.20 AS.reconfigure ASconfig @04:53:17.31 AS.seek 1ml @04:54:15.09 delay 1 second @04:54:16.11 AFV.select :holdingCoil @04:54:16.21 delay 1 second @04:54:17.23 AS.reconfigure ASfast @04:54:17.28 AS.seek 0ml @04:54:44.69 delay 3 seconds @04:54:47.71 AFV.select :carrierSolution @04:54:47.81 delay 2 seconds @04:54:49.83 AS.reconfigure ASconfig @04:54:49.89 AS.seek 1ml @04:55:47.64 delay 1 second @04:55:48.66 AFV.select :holdingCoil @04:55:48.76 delay 1 second @04:55:49.78 AS.reconfigure ASfast @04:55:49.83 AS.seek 0ml @04:56:17.26 delay 3 seconds @04:56:20.28 AFV.select :carrierSolution @04:56:20.38 delay 2 seconds @04:56:22.40 AS.reconfigure ASconfig @04:56:22.45 AS.seek 1ml @04:57:20.23 delay 1 second @04:57:21.25 AFV.select :holdingCoil @04:57:21.35 delay 1 second @04:57:22.37 AS.reconfigure ASfast @04:57:22.42 AS.seek 0ml @04:57:49.78 delay 3 seconds @04:57:52.80 AFV.select :carrierSolution @04:57:52.90 delay 2 seconds @04:57:54.92 AS.reconfigure ASconfig @04:57:54.97 AS.seek 1ml @04:58:52.76 delay 1 second @04:58:53.78 AFV.select :holdingCoil @04:58:53.88 delay 1 second @04:58:54.90 AS.reconfigure ASnorm @04:58:54.95 AS.seek 0ml @04:59:45.67
AFV.select :carrierSolution @04:59:45.77 delay 2 seconds @04:59:47.79 AS.reconfigure ASconfig @04:59:47.84 AS.seek 0.5ml @05:00:16.91 delay 1 second @05:00:17.93 AFV.select :holdingCoil @05:00:18.03 ATV.dial :air1 @05:00:19.53 AS.reconfigure ASslow2 @05:00:19.57 AS.seek 0.506ml @05:00:25.76 ATV.dial :extSrc @05:00:27.47 AS.seek 0.512ml @05:00:33.66 delay 5 seconds @05:00:38.68 ATV.dial :air1 @05:00:40.39 AS.reconfigure ASslow1 @05:00:40.44 AS.seek 0.542ml @05:00:46.72 ATV.dial :waste @05:00:47.26 AS.reconfigure ASconfig @05:00:47.30 AS.seek :empty @05:01:18.92 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118dec1405h.pcr @05:01:18.94 Building PCR Slug from extSrc with MM2 mix, PP4 primer @05:01:18.95 Priming with carrier @05:01:18.96 ATV.dial ARV @05:01:20.45 ARV.dial :carrier @05:01:20.79 AS.reconfigure ASnorm @05:01:20.83 AS.seek 0.499ml @05:01:46.38 AFV.select :carrierSolution @05:01:46.49 delay 2 seconds @05:01:48.51 AS.reconfigure ASconfig @05:01:48.55 AS.seek 1ml @05:02:17.61 delay 1 second @05:02:18.63 AFV.select :holdingCoil @05:02:18.73 delay 1 second @05:02:19.75 ATV.dial :waste @05:02:21.42 AS.reconfigure ASnorm @05:02:21.46 AS.seek :empty @05:03:12.21 AFV.select :carrierSolution @05:03:12.32 delay 2 seconds @05:03:14.35 AS.reconfigure ASconfig @05:03:14.39 AS.seek 0.5ml @05:03:43.47 delay 1 second @05:03:44.49 AFV.select :holdingCoil @05:03:44.59 Building Slug @05:03:44.60 ATV.dial :ARV @05:03:46.25 delay 2 seconds @05:03:48.27 ARV.dial :air1 @05:03:48.67 delay 2 seconds @05:03:50.74 AS.reconfigure ASslow2 @05:03:50.78 AS.seek 0.506ml @05:03:57.06 delay 2 seconds @05:03:59.08 ARV.dial :MM2 @05:04:00.59 delay 2 seconds @05:04:02.61 Adding MM2 @05:04:02.67 AS.seek 0.524ml @05:04:20.98 delay 25 seconds @05:04:46.00 ARV.dial :PP4 @05:04:46.39 Adding PP4 @05:04:46.40 delay 2 seconds @05:04:48.47 AS.seek 0.53ml @05:04:54.81 delay 10 seconds @05:05:04.84 ARV.dial :air3 @05:05:05.23 adding ARV air @05:05:05.24 delay 2 seconds @05:05:07.31 AS.seek 0.536ml @05:05:13.56 delay 5 seconds @05:05:18.58 ARV.dial :carrier @05:05:19.87 delay 7 seconds @05:05:26.95 AS.seek 0.594ml @05:06:25.42 delay 5 seconds @05:06:30.44 ATV.dial :extSrc @05:06:31.00 delay 2 seconds @05:06:33.07 AS.seek 0.6ml @05:06:39.69 delay 4 seconds @05:06:43.71 ATV.dial ARV @05:06:44.27 delay 2 seconds @05:06:46.29 ARV.dial :carrier @05:06:46.30 delay 2 seconds @05:06:48.37 AS.seek 0.65ml @05:07:38.84 delay 4 seconds @05:07:42.86 ATV.dial PCR @05:07:43.62 ASV.dial :waste1 @05:07:43.63 ARV.dial :air1 @05:07:44.03 Positioning Slug by 0.324ml @05:07:44.10 AS.seek 0.326ml @05:13:10.19 delay 5 seconds @05:13:15.21 ATV.dial ARV @05:13:15.98 ASV.dial :PCRlock @05:13:16.38 delay 5 seconds @05:13:21.40 Running Tt1 Profile60C300 thermocycling @05:13:21.41 PCR.program Profile60C300 @05:13:21.53 Profile60C300 should complete in < 1:45:21 @05:13:21.54 PCR.run 2:16:41.25 @06:34:48.14 PCR.off @06:34:49.64 PCR for Tt1 completed -- closed /var/log/esp/Tt118dec1405h.pcr @06:34:49.70 Cleaning PCR @06:34:49.71 AFV.select :carrierSolution @06:34:49.81 delay 2 seconds @06:34:51.83 AS.reconfigure ASconfig @06:34:51.88 AS.seek 0.5ml @06:35:02.16 delay 1 second @06:35:03.18 AFV.select :holdingCoil @06:35:03.28 ASV.dial :waste1 @06:35:03.68 ATV.dial :air3 @06:35:04.96 AS.reconfigure ASslow1 @06:35:05.01 AS.seek 0.51ml @06:35:07.27 delay 2 seconds @06:35:09.29 ATV.dial :bleach @06:35:09.91 AS.seek 0.81ml @06:36:10.49 delay 3 seconds @06:36:13.51 ATV.dial :air3 @06:36:14.05 delay 3 seconds @06:36:17.12 AS.seek 0.835ml @06:36:22.38 delay 3 seconds @06:36:25.40 ATV.dial :bleach @06:36:26.41 AS.seek 0.82ml @06:36:29.69 delay 3 seconds @06:36:32.71 ARV.dial :waste @06:36:33.32 delay 3 seconds @06:36:36.34 ATV.dial :ARV @06:36:37.23 AS.reconfigure ASconfig @06:36:37.28 AS.seek 0.47ml @06:36:57.70 AFV.select :carrierSolution @06:36:57.81 delay 2 seconds @06:36:59.84 AS.seek 1ml @06:37:30.60 delay 1 second @06:37:31.62 AFV.select :holdingCoil @06:37:31.72 delay 1 second @06:37:32.74 AS.reconfigure ASfast @06:37:32.79 AS.seek 0ml @06:38:00.14 AFV.select :carrierSolution @06:38:00.25 delay 2 seconds @06:38:02.27 AS.reconfigure ASconfig @06:38:02.32 AS.seek 1ml @06:39:00.10 delay 1 second @06:39:01.12 AFV.select :holdingCoil @06:39:01.22 delay 1 second @06:39:02.24 AS.reconfigure ASfast @06:39:02.29 AS.seek 0ml @06:39:29.74 AFV.select :carrierSolution @06:39:29.84 delay 2 seconds @06:39:31.86 AS.reconfigure ASconfig @06:39:31.91 AS.seek 1ml @06:40:29.69 delay 1 second @06:40:30.71 AFV.select :holdingCoil @06:40:30.81 delay 1 second @06:40:31.83 AS.reconfigure ASfast @06:40:31.88 AS.seek 0ml @06:40:59.26 ARV.dial between carrier and air1 @06:40:59.76 ATV.dial :air3 @06:41:01.04 AS.reconfigure ASslow1 @06:41:01.09 AS.seek 0.01ml @06:41:03.43 delay 2 seconds @06:41:05.45 ATV.dial :bleach @06:41:06.07 AS.seek 0.81ml @06:43:47.24 delay 3 seconds @06:43:50.26 ATV.dial :air3 @06:43:50.82 delay 3 seconds @06:43:53.89 AS.seek 0.835ml @06:43:59.22 delay 3 seconds @06:44:02.24 ATV.dial :bleach @06:44:02.86 AS.seek 0.82ml @06:44:06.15 delay 3 seconds @06:44:09.17 ATV.dial PCR @06:44:09.55 AS.reconfigure ASconfig @06:44:09.59 AS.seek :empty @06:44:57.07 PCR.program :bleachProfile @06:44:57.11 PCR program should complete in < 2:45 @06:44:57.12 PCR.run 10:00 @06:47:55.91 PCR.off @06:47:55.93 AFV.select :carrierSolution @06:47:56.03 delay 2 seconds @06:47:58.06 AS.seek 1ml @06:48:55.85 delay 1 second @06:48:56.87 AFV.select :holdingCoil @06:48:56.97 delay 1 second @06:48:57.99 AS.reconfigure ASfast @06:48:58.04 AS.seek 0ml @06:49:25.38 delay 3 seconds @06:49:28.40 AFV.select :carrierSolution @06:49:28.50 delay 2 seconds @06:49:30.52 AS.reconfigure ASconfig @06:49:30.57 AS.seek 1ml @06:50:28.32 delay 1 second @06:50:29.34 AFV.select :holdingCoil @06:50:29.44 delay 1 second @06:50:30.46 AS.reconfigure ASfast @06:50:30.51 AS.seek 0ml @06:50:58.04 delay 3 seconds @06:51:01.06 AFV.select :carrierSolution @06:51:01.16 delay 2 seconds @06:51:03.18 AS.reconfigure ASconfig @06:51:03.24 AS.seek 1ml @06:52:01.02 delay 1 second @06:52:02.04 AFV.select :holdingCoil @06:52:02.14 delay 1 second @06:52:03.16 AS.reconfigure ASfast @06:52:03.21 AS.seek 0ml @06:52:30.57 delay 3 seconds @06:52:33.59 AFV.select :carrierSolution @06:52:33.69 delay 2 seconds @06:52:35.71 AS.reconfigure ASconfig @06:52:35.76 AS.seek 1ml @06:53:33.51 delay 1 second @06:53:34.53 AFV.select :holdingCoil @06:53:34.63 delay 1 second @06:53:35.65 AS.reconfigure ASfast @06:53:35.70 AS.seek 0ml @06:54:03.07 delay 3 seconds @06:54:06.09 AFV.select :carrierSolution @06:54:06.19 delay 2 seconds @06:54:08.21 AS.reconfigure ASconfig @06:54:08.27 AS.seek 1ml @06:55:06.02 delay 1 second @06:55:07.04 AFV.select :holdingCoil @06:55:07.14 delay 1 second @06:55:08.17 AS.reconfigure ASnorm @06:55:08.21 AS.seek 0ml @06:55:58.92
deconEluateRecoveryLine extSrc @06:55:58.93 ATV.dial :waste @06:56:00.59 AS.seek 0ml @06:56:00.66 ATV.dial :air3 @06:56:01.49 AS.reconfigure ASslow1 @06:56:01.53 AS.seek 0.01ml @06:56:03.85 delay 2 seconds @06:56:05.87 ATV.dial :bleach @06:56:06.32 AS.seek 0.31ml @06:57:06.93 delay 3 seconds @06:57:09.95 ATV.dial :air3 @06:57:10.49 delay 3 seconds @06:57:13.56 AS.seek 0.335ml @06:57:18.88 delay 3 seconds @06:57:21.90 ATV.dial :bleach @06:57:22.51 AS.seek 0.32ml @06:57:25.81 delay 3 seconds @06:57:28.83 ATV.dial :extSrc @06:57:29.45 AS.seek 0ml @06:58:34.05 AFV.select :carrierSolution @06:58:34.16 delay 2 seconds @06:58:36.18 AS.reconfigure ASconfig @06:58:36.23 AS.seek 0.5ml @06:59:05.26 delay 1 second @06:59:06.28 AFV.select :holdingCoil @06:59:06.38 ATV.dial :extSrc @06:59:06.40 AS.reconfigure ASslow1 @06:59:06.44 AS.seek 0ml @07:00:47.25 AFV.select :carrierSolution @07:00:47.36 delay 2 seconds @07:00:49.38 AS.reconfigure ASconfig @07:00:49.43 AS.seek 0.5ml @07:01:18.46 delay 1 second @07:01:19.48 AFV.select :holdingCoil @07:01:19.58 ATV.dial :extSrc @07:01:19.60 AS.reconfigure ASslow1 @07:01:19.64 AS.seek 0ml @07:03:00.46 AFV.select :carrierSolution @07:03:00.56 delay 2 seconds @07:03:02.58 AS.reconfigure ASconfig @07:03:02.63 AS.seek 0.5ml @07:03:31.66 delay 1 second @07:03:32.68 AFV.select :holdingCoil @07:03:32.78 ATV.dial :extSrc @07:03:32.80 AS.reconfigure ASslow1 @07:03:32.84 AS.seek 0ml @07:05:13.62 AFV.select :carrierSolution @07:05:13.73 delay 2 seconds @07:05:15.75 AS.reconfigure ASconfig @07:05:15.80 AS.seek 0.5ml @07:05:44.82 delay 1 second @07:05:45.84 AFV.select :holdingCoil @07:05:45.94 ATV.dial :extSrc @07:05:45.96 AS.reconfigure ASslow1 @07:05:46.00 AS.seek 0ml @07:07:26.82 ATV.dial :air2 @07:07:28.15 AS.reconfigure ASslow0 @07:07:28.20 AS.seek 0.5ml @07:08:18.83 ATV.dial :extSrc @07:08:20.33 AS.seek 0.02ml @07:09:08.93 delay 1 second @07:09:09.95 ATV.dial :waste @07:09:11.24 AS.reconfigure ASconfig @07:09:11.28 AS.seek :empty @07:09:12.77 deconEluateRecoveryLine extSrc ATV.dial :waste @07:09:12.79 AS.reconfigure ASnorm @07:09:12.83 AS.seek 0ml @07:09:13.17 ATV.dial :air3 @07:09:14.00 AS.reconfigure ASslow1 @07:09:14.04 AS.seek 0.01ml @07:09:16.31 delay 2 seconds @07:09:18.33 ATV.dial :bleach @07:09:18.77 AS.seek 0.31ml @07:10:19.37 delay 3 seconds @07:10:22.39 ATV.dial :air3 @07:10:22.93 delay 3 seconds @07:10:26.00 AS.seek 0.335ml @07:10:31.32 delay 3 seconds @07:10:34.34 ATV.dial :bleach @07:10:34.94 AS.seek 0.32ml @07:10:38.23 delay 3 seconds @07:10:41.25 ATV.dial :extSrc @07:10:41.85 AS.seek 0ml @07:11:46.45 AFV.select :carrierSolution @07:11:46.56 delay 2 seconds @07:11:48.58 AS.reconfigure ASconfig @07:11:48.63 AS.seek 0.5ml @07:12:17.67 delay 1 second @07:12:18.69 AFV.select :holdingCoil @07:12:18.79 ATV.dial :extSrc @07:12:18.81 AS.reconfigure ASslow1 @07:12:18.85 AS.seek 0ml @07:13:59.64 AFV.select :carrierSolution @07:13:59.75 delay 2 seconds @07:14:01.77 AS.reconfigure ASconfig @07:14:01.82 AS.seek 0.5ml @07:14:30.85 delay 1 second @07:14:31.87 AFV.select :holdingCoil @07:14:31.97 ATV.dial :extSrc @07:14:31.99 AS.reconfigure ASslow1 @07:14:32.03 AS.seek 0ml @07:16:12.83 AFV.select :carrierSolution @07:16:12.94 delay 2 seconds @07:16:14.96 AS.reconfigure ASconfig @07:16:15.01 AS.seek 0.5ml @07:16:44.04 delay 1 second @07:16:45.06 AFV.select :holdingCoil @07:16:45.16 ATV.dial :extSrc @07:16:45.18 AS.reconfigure ASslow1 @07:16:45.22 AS.seek 0ml @07:18:26.02 AFV.select :carrierSolution @07:18:26.13 delay 2 seconds @07:18:28.15 AS.reconfigure ASconfig @07:18:28.20 AS.seek 0.5ml @07:18:57.59 delay 1 second @07:18:58.61 AFV.select :holdingCoil @07:18:58.71 ATV.dial :extSrc @07:18:58.73 AS.reconfigure ASslow1 @07:18:58.77 AS.seek 0ml @07:20:39.59 ATV.dial :air2 @07:20:40.91 AS.reconfigure ASslow0 @07:20:40.95 AS.seek 0.5ml @07:21:31.54 ATV.dial :extSrc @07:21:33.02 AS.seek 0.02ml @07:22:21.60 delay 1 second @07:22:22.62 ATV.dial :waste @07:22:23.92 AS.reconfigure ASconfig @07:22:23.96 AS.seek :empty @07:22:25.48 deconEluateRecoveryLine extSrc @07:22:25.49 ATV.dial :waste @07:22:25.50 AS.reconfigure ASnorm @07:22:25.54 AS.seek 0ml @07:22:25.89 ATV.dial :air3 @07:22:26.72 AS.reconfigure ASslow1 @07:22:26.76 AS.seek 0.01ml @07:22:29.04 delay 2 seconds @07:22:31.06 ATV.dial :bleach @07:22:31.51 AS.seek 0.31ml @07:23:32.12 delay 3 seconds @07:23:35.14 ATV.dial :air3 @07:23:35.70 delay 3 seconds @07:23:38.77 AS.seek 0.335ml @07:23:44.10 delay 3 seconds @07:23:47.12 ATV.dial :bleach @07:23:47.74 AS.seek 0.32ml @07:23:51.04 delay 3 seconds @07:23:54.06 ATV.dial :extSrc @07:23:54.67 AS.seek 0ml @07:24:59.27 AFV.select :carrierSolution @07:24:59.38 delay 2 seconds @07:25:01.40 AS.reconfigure ASconfig @07:25:01.45 AS.seek 0.5ml @07:25:30.48 delay 1 second @07:25:31.50 AFV.select :holdingCoil @07:25:31.60 ATV.dial :extSrc @07:25:31.62 AS.reconfigure ASslow1 @07:25:31.66 AS.seek 0ml @07:27:12.47 AFV.select :carrierSolution @07:27:12.58 delay 2 seconds @07:27:14.60 AS.reconfigure ASconfig @07:27:14.65 AS.seek 0.5ml @07:27:43.68 delay 1 second @07:27:44.70 AFV.select :holdingCoil @07:27:44.80 ATV.dial :extSrc @07:27:44.82 AS.reconfigure ASslow1 @07:27:44.86 AS.seek 0ml @07:29:25.67 AFV.select :carrierSolution @07:29:25.78 delay 2 seconds @07:29:27.80 AS.reconfigure ASconfig @07:29:27.85 AS.seek 0.5ml @07:29:56.90 delay 1 second @07:29:57.92 AFV.select :holdingCoil @07:29:58.02 ATV.dial :extSrc @07:29:58.04 AS.reconfigure ASslow1 @07:29:58.08 AS.seek 0ml @07:31:38.89 AFV.select :carrierSolution @07:31:39.00 delay 2 seconds @07:31:41.02 AS.reconfigure ASconfig @07:31:41.07 AS.seek 0.5ml @07:32:10.07 delay 1 second @07:32:11.09 AFV.select :holdingCoil @07:32:11.19 ATV.dial :extSrc @07:32:11.21 AS.reconfigure ASslow1 @07:32:11.25 AS.seek 0ml @07:33:52.09 ATV.dial :air2 @07:33:53.41 AS.reconfigure ASslow0 @07:33:53.46 AS.seek 0.5ml @07:34:44.05 ATV.dial :extSrc @07:34:45.54 AS.seek 0.02ml @07:35:34.14 delay 1 second @07:35:35.16 ATV.dial :waste @07:35:36.46 AS.reconfigure ASconfig @07:35:36.50 AS.seek :empty @14:51:05.99CET18-Dec-18
ESP2 v3.30 I2C Gateway v3.06 for core bus @14:51:07.65 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @14:51:07.92 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @14:51:12.24 Polling Can every 10:00 @14:51:13.99 SPE.coast @14:51:14.04 SPE.configure SPEconfig @14:51:14.12 AS.reconfigure ASconfig @14:51:14.16 AS.hold @14:51:14.22 AS.configure ASconfig @14:51:14.30 SS.reconfigure SSconfig @14:51:14.34 SS.hold @14:51:14.39 SS.configure SSconfig @14:51:14.47 CC.reconfigure CCconfig @14:51:14.51 CC.hold @14:51:14.57 CC.configure CCconfig @14:51:14.65 CS.reconfigure CSconfig @14:51:14.68 CS.hold @14:51:14.76 CS.configure CSconfig @14:51:15.17 PC.reconfigure PCconfig @14:51:15.21 PC.hold @14:51:15.28 PC.configure PCconfig @14:51:15.36 PS.configure PSconfig @14:51:15.46 Forearm.reconfigure LooseForearm @14:51:15.49 Forearm.hold @14:51:15.54 Forearm.configure LooseForearm @14:51:15.62 Elbow.reconfigure LooseElbow @14:51:15.66 Elbow.hold @14:51:15.72 Elbow.configure LooseElbow @14:51:15.82 SC.reconfigure SCloose @14:51:15.86 SC.hold @14:51:15.92 SC.configure SCloose @14:51:16.00 SE.reconfigure SEconfig @14:51:16.04 SE.hold @14:51:16.08 SE.configure SEconfig @14:51:16.12 CH.coast @14:51:16.17 CH.configure CHconfig @14:51:16.19 PH.coast @14:51:16.24 PH.configure PHconfig @14:51:16.28 PCR.off @14:51:16.31 PCR.pidtemp= 50,0.1,0,300,25,25 @14:51:16.33 PCR.caltemp= 2.7073,0.0373,0 @14:51:16.36 All dwarf controllers running firmware version 3.75 @14:51:16.48 Cleanly exited at 14:42:44CET18-Dec-18 @14:51:16.51 Loading /home/esp/esp2/mission/stdMER2017_MWJ.rb mission @14:51:17.02 AFV.select :carrierSolution @14:51:17.12 delay 2 seconds @14:51:18.17 Session Begun @14:51:19.15
AS.seek 0.5ml @14:51:48.28 delay 1 second @14:51:49.30 AFV.select :holdingCoil @14:51:49.41 ATV.dial :extSrc @14:51:50.91 AS.reconfigure ASslow1 @14:51:50.96 AS.seek 0.53ml @14:51:57.31 delay 5 seconds @14:52:02.39 AS.reconfigure ASslow2 @14:52:02.45 AS.seek 0.54ml @14:52:12.69 delay 20 seconds @14:52:32.76 AS.reconfigure ASslow1 @14:52:32.82 AS.seek 0.57ml @14:52:39.11 delay 5 seconds @14:52:44.19 AS.reconfigure ASslow2 @14:52:44.25 AS.seek 0.58ml @14:52:54.44 delay 20 seconds @14:53:14.52 AS.reconfigure ASslow1 @14:53:14.58 AS.seek 0.61ml @14:53:20.92 delay 5 seconds @14:53:25.99 AS.reconfigure ASslow2 @14:53:26.05 AS.seek 0.62ml @14:53:36.33 delay 20 seconds @14:53:56.40 AS.reconfigure ASslow1 @14:53:56.48 AS.seek 0.65ml @14:54:02.81 delay 5 seconds @14:54:07.89 AS.reconfigure ASslow2 @14:54:07.95 AS.seek 0.66ml @14:54:18.19 delay 20 seconds @14:54:38.23 AS.reconfigure ASslow1 @14:54:38.28 AS.seek 0.705ml @14:54:47.60 delay 10 seconds @14:54:57.62 ATV.dial :air1 @14:54:59.34 AS.seek 0.725ml @14:55:03.61 delay 3 seconds @14:55:06.63 ATV.dial :waste @14:55:07.19 AS.reconfigure ASslow0 @14:55:07.25 AS.seek :empty @14:56:20.69 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118dec1814h.pcr @14:56:20.71 Building PCR Slug from extSrc with MM2 mix, PP4 primer @14:56:20.74 Priming with carrier @14:56:20.75 ATV.dial ARV @14:56:22.27 ARV.dial :carrier @14:56:22.62 AS.reconfigure ASnorm @14:56:22.68 AS.seek 0.499ml @14:56:48.24 AFV.select :carrierSolution @14:56:48.35 delay 2 seconds @14:56:50.38 AS.reconfigure ASconfig @14:56:50.43 AS.seek 1ml @14:57:19.52 delay 1 second @14:57:20.54 AFV.select :holdingCoil @14:57:20.64 delay 1 second @14:57:21.66 ATV.dial :waste @14:57:23.33 AS.reconfigure ASnorm @14:57:23.39 AS.seek :empty @14:58:14.14 AFV.select :carrierSolution @14:58:14.25 delay 2 seconds @14:58:16.28 AS.reconfigure ASconfig @14:58:16.33 AS.seek 0.5ml @14:58:45.46 delay 1 second @14:58:46.48 AFV.select :holdingCoil @14:58:46.58 Building Slug @14:58:46.59 ATV.dial :ARV @14:58:48.36 delay 2 seconds @14:58:50.38 ARV.dial :air1 @14:58:50.79 delay 2 seconds @14:58:52.86 AS.reconfigure ASslow2 @14:58:52.92 AS.seek 0.506ml @14:58:59.21 delay 2 seconds @14:59:01.23 ARV.dial :MM2 @14:59:02.75 delay 2 seconds @14:59:04.77 Adding MM2 @14:59:04.86 AS.seek 0.524ml @14:59:23.16 delay 25 seconds @14:59:48.18 ARV.dial :PP4 @14:59:48.58 Adding PP4 @14:59:48.59 delay 2 seconds @14:59:50.67 AS.seek 0.53ml @14:59:56.89 delay 10 seconds @15:00:06.92 ARV.dial :air3 @15:00:07.33 adding ARV air @15:00:07.34 delay 2 seconds @15:00:09.42 AS.seek 0.536ml @15:00:15.60 delay 5 seconds @15:00:20.62 ARV.dial :carrier @15:00:21.92 delay 7 seconds @15:00:29.00 AS.seek 0.594ml @15:01:27.72 delay 5 seconds @15:01:32.74 ATV.dial :extSrc @15:01:33.28 delay 2 seconds @15:01:35.35 AS.seek 0.6ml @15:01:41.99 delay 4 seconds @15:01:46.01 ATV.dial ARV @15:01:46.66 delay 2 seconds @15:01:48.68 ARV.dial :carrier @15:01:48.69 delay 2 seconds @15:01:50.77 AS.seek 0.65ml @15:02:41.41 delay 4 seconds @15:02:45.43 ATV.dial PCR @15:02:46.30 ASV.dial :waste1 @15:02:46.39 ARV.dial :air1 @15:02:47.35 Positioning Slug by 0.324ml @15:02:47.44 AS.seek 0.326ml @15:08:13.46 delay 5 seconds @15:08:18.48 ATV.dial ARV @15:08:19.25 ASV.dial :PCRlock @15:08:19.66 delay 5 seconds @15:08:24.68 Running Tt1 Profile60C300 thermocycling @15:08:24.70 PCR.program Profile60C300 @15:08:24.84 Profile60C300 should complete in < 1:45:21 @15:08:24.85 PCR.run 2:16:41.25 @16:30:26.65 PCR.off @16:30:28.16 PCR for Tt1 completed -- closed /var/log/esp/Tt118dec1814h.pcr @16:30:28.24 Cleaning PCR @16:30:28.25 AFV.select :carrierSolution @16:30:28.37 delay 2 seconds @16:30:30.40 AS.reconfigure ASconfig @16:30:30.45 AS.seek 0.5ml @16:30:40.76 delay 1 second @16:30:41.78 AFV.select :holdingCoil @16:30:41.88 ASV.dial :waste1 @16:30:42.27 ATV.dial :air3 @16:30:43.56 AS.reconfigure ASslow1 @16:30:43.62 AS.seek 0.51ml @16:30:45.91 delay 2 seconds @16:30:47.94 ATV.dial :bleach @16:30:48.55 AS.seek 0.81ml @16:31:49.16 delay 3 seconds @16:31:52.18 ATV.dial :air3 @16:31:52.74 delay 3 seconds @16:31:55.81 AS.seek 0.835ml @16:32:01.13 delay 3 seconds @16:32:04.15 ATV.dial :bleach @16:32:04.77 AS.seek 0.82ml @16:32:08.06 delay 3 seconds @16:32:11.08 ARV.dial :waste @16:32:11.70 delay 3 seconds @16:32:14.72 ATV.dial :ARV @16:32:15.61 AS.reconfigure ASconfig @16:32:15.68 AS.seek 0.47ml @16:32:36.13 AFV.select :carrierSolution @16:32:36.24 delay 2 seconds @16:32:38.27 AS.seek 1ml @16:33:09.02 delay 1 second @16:33:10.04 AFV.select :holdingCoil @16:33:10.15 delay 1 second @16:33:11.18 AS.reconfigure ASfast @16:33:11.23 AS.seek 0ml @16:33:38.68 AFV.select :carrierSolution @16:33:38.79 delay 2 seconds @16:33:40.82 AS.reconfigure ASconfig @16:33:40.87 AS.seek 1ml @16:34:38.65 delay 1 second @16:34:39.67 AFV.select :holdingCoil @16:34:39.78 delay 1 second @16:34:40.81 AS.reconfigure ASfast @16:34:40.86 AS.seek 0ml @16:35:08.51 AFV.select :carrierSolution @16:35:08.63 delay 2 seconds @16:35:10.66 AS.reconfigure ASconfig @16:35:10.72 AS.seek 1ml @16:36:08.48 delay 1 second @16:36:09.50 AFV.select :holdingCoil @16:36:09.60 delay 1 second @16:36:10.63 AS.reconfigure ASfast @16:36:10.68 AS.seek 0ml @16:36:38.04 ARV.dial between carrier and air1 @16:36:38.56 ATV.dial :air3 @16:36:39.87 AS.reconfigure ASslow1 @16:36:39.93 AS.seek 0.01ml @16:36:42.27 delay 2 seconds @16:36:44.29 ATV.dial :bleach @16:36:44.91 AS.seek 0.81ml @16:39:26.06 delay 3 seconds @16:39:29.08 ATV.dial :air3 @16:39:29.65 delay 3 seconds @16:39:32.72 AS.seek 0.835ml @16:39:38.01 delay 3 seconds @16:39:41.03 ATV.dial :bleach @16:39:41.65 AS.seek 0.82ml @16:39:44.96 delay 3 seconds @16:39:47.98 ATV.dial PCR @16:39:48.38 AS.reconfigure ASconfig @16:39:48.44 AS.seek :empty @16:40:35.90 PCR.program :bleachProfile @16:40:35.95 PCR program should complete in < 2:45 @16:40:35.96 PCR.run 10:00 @16:43:34.77 PCR.off @16:43:34.80 AFV.select :carrierSolution @16:43:34.92 delay 2 seconds @16:43:36.95 AS.seek 1ml @16:44:34.79 delay 1 second @16:44:35.81 AFV.select :holdingCoil @16:44:35.91 delay 1 second @16:44:36.94 AS.reconfigure ASfast @16:44:36.99 AS.seek 0ml @16:45:04.35 delay 3 seconds @16:45:07.37 AFV.select :carrierSolution @16:45:07.47 delay 2 seconds @16:45:09.50 AS.reconfigure ASconfig @16:45:09.56 AS.seek 1ml @16:46:07.32 delay 1 second @16:46:08.34 AFV.select :holdingCoil @16:46:08.45 delay 1 second @16:46:09.48 AS.reconfigure ASfast @16:46:09.53 AS.seek 0ml @16:46:36.90 delay 3 seconds @16:46:39.92 AFV.select :carrierSolution @16:46:40.02 delay 2 seconds @16:46:42.05 AS.reconfigure ASconfig @16:46:42.10 AS.seek 1ml @16:47:39.85 delay 1 second @16:47:40.87 AFV.select :holdingCoil @16:47:40.98 delay 1 second @16:47:42.01 AS.reconfigure ASfast @16:47:42.06 AS.seek 0ml @16:48:09.62 delay 3 seconds @16:48:12.64 AFV.select :carrierSolution @16:48:12.74 delay 2 seconds @16:48:14.77 AS.reconfigure ASconfig @16:48:14.83 AS.seek 1ml @16:49:12.62 delay 1 second @16:49:13.64 AFV.select :holdingCoil @16:49:13.74 delay 1 second @16:49:14.77 AS.reconfigure ASfast @16:49:14.82 AS.seek 0ml @16:49:42.17 delay 3 seconds @16:49:45.19 AFV.select :carrierSolution @16:49:45.30 delay 2 seconds @16:49:47.33 AS.reconfigure ASconfig @16:49:47.39 AS.seek 1ml @16:50:45.17 delay 1 second @16:50:46.19 AFV.select :holdingCoil @16:50:46.29 delay 1 second @16:50:47.32 AS.reconfigure ASnorm @16:50:47.37 AS.seek 0ml @16:51:38.08
AFV.select :carrierSolution @16:51:38.19 delay 2 seconds @16:51:40.22 AS.reconfigure ASconfig @16:51:40.27 AS.seek 0.5ml @16:52:09.35 delay 1 second @16:52:10.37 AFV.select :holdingCoil @16:52:10.48 ATV.dial :air1 @16:52:11.99 AS.reconfigure ASslow2 @16:52:12.05 AS.seek 0.506ml @16:52:18.26 ATV.dial :extSrc @16:52:19.98 AS.seek 0.512ml @16:52:26.60 delay 5 seconds @16:52:31.62 ATV.dial :air1 @16:52:33.34 AS.reconfigure ASslow1 @16:52:33.40 AS.seek 0.542ml @16:52:39.76 ATV.dial :waste @16:52:40.32 AS.reconfigure ASconfig @16:52:40.38 AS.seek :empty @16:53:12.02 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118dec1816h.pcr @16:53:12.05 Building PCR Slug from extSrc with MM2 mix, PP4 primer @16:53:12.07 Priming with carrier @16:53:12.09 ATV.dial ARV @16:53:13.70 ARV.dial :carrier @16:53:14.05 AS.reconfigure ASnorm @16:53:14.11 AS.seek 0.499ml @16:53:39.71 AFV.select :carrierSolution @16:53:39.82 delay 2 seconds @16:53:41.85 AS.reconfigure ASconfig @16:53:41.90 AS.seek 1ml @16:54:10.98 delay 1 second @16:54:12.00 AFV.select :holdingCoil @16:54:12.10 delay 1 second @16:54:13.12 ATV.dial :waste @16:54:14.79 AS.reconfigure ASnorm @16:54:14.85 AS.seek :empty @16:55:05.59 AFV.select :carrierSolution @16:55:05.70 delay 2 seconds @16:55:07.73 AS.reconfigure ASconfig @16:55:07.78 AS.seek 0.5ml @16:55:36.92 delay 1 second @16:55:37.94 AFV.select :holdingCoil @16:55:38.04 Building Slug @16:55:38.05 ATV.dial :ARV @16:55:39.71 delay 2 seconds @16:55:41.73 ARV.dial :air1 @16:55:42.13 delay 2 seconds @16:55:44.20 AS.reconfigure ASslow2 @16:55:44.26 AS.seek 0.506ml @16:55:50.46 delay 2 seconds @16:55:52.48 ARV.dial :MM2 @16:55:53.99 delay 2 seconds @16:55:56.01 Adding MM2 @16:55:56.09 AS.seek 0.524ml @16:56:14.40 delay 25 seconds @16:56:39.75 ARV.dial :PP4 @16:56:40.23 Adding PP4 @16:56:40.24 delay 2 seconds @16:56:42.32 AS.seek 0.53ml @16:56:48.56 delay 10 seconds @16:56:58.59 ARV.dial :air3 @16:56:58.99 adding ARV air @16:56:59.00 delay 2 seconds @16:57:01.08 AS.seek 0.536ml @16:57:07.68 delay 5 seconds @16:57:12.70 ARV.dial :carrier @16:57:14.01 delay 7 seconds @16:57:21.09 AS.seek 0.594ml @16:58:19.85 delay 5 seconds @16:58:24.87 ATV.dial :extSrc @16:58:25.43 delay 2 seconds @16:58:27.50 AS.seek 0.6ml @16:58:33.87 delay 4 seconds @16:58:37.89 ATV.dial ARV @16:58:38.45 delay 2 seconds @16:58:40.47 ARV.dial :carrier @16:58:40.48 delay 2 seconds @16:58:42.56 AS.seek 0.65ml @16:59:33.23 delay 4 seconds @16:59:37.25 ATV.dial PCR @16:59:38.04 ASV.dial :waste1 @16:59:38.05 ARV.dial :air1 @16:59:38.47 Positioning Slug by 0.324ml @16:59:38.56 AS.seek 0.326ml @17:05:04.60 delay 5 seconds @17:05:09.62 ATV.dial ARV @17:05:10.42 ASV.dial :PCRlock @17:05:10.82 delay 5 seconds @17:05:15.84 Running Tt1 Profile60C300 thermocycling @17:05:15.85 PCR.program Profile60C300 @17:05:16.00 Profile60C300 should complete in < 1:45:21 @17:05:16.01 PCR.run 2:16:41.25 @18:27:03.38 PCR.off @18:27:04.88 PCR for Tt1 completed -- closed /var/log/esp/Tt118dec1816h.pcr @18:27:04.95 Cleaning PCR @18:27:04.96 AFV.select :carrierSolution @18:27:05.08 delay 2 seconds @18:27:07.11 AS.reconfigure ASconfig @18:27:07.16 AS.seek 0.5ml @18:27:17.48 delay 1 second @18:27:18.50 AFV.select :holdingCoil @18:27:18.60 ASV.dial :waste1 @18:27:19.00 ATV.dial :air3 @18:27:20.30 AS.reconfigure ASslow1 @18:27:20.37 AS.seek 0.51ml @18:27:22.65 delay 2 seconds @18:27:25.00 ATV.dial :bleach @18:27:25.69 AS.seek 0.81ml @18:28:26.30 delay 3 seconds @18:28:29.32 ATV.dial :air3 @18:28:29.88 delay 3 seconds @18:28:32.95 AS.seek 0.835ml @18:28:38.23 delay 3 seconds @18:28:41.25 ATV.dial :bleach @18:28:41.87 AS.seek 0.82ml @18:28:45.11 delay 3 seconds @18:28:48.13 ARV.dial :waste @18:28:48.75 delay 3 seconds @18:28:51.77 ATV.dial :ARV @18:28:52.66 AS.reconfigure ASconfig @18:28:52.73 AS.seek 0.47ml @18:29:13.13 AFV.select :carrierSolution @18:29:13.24 delay 2 seconds @18:29:15.27 AS.seek 1ml @18:29:46.05 delay 1 second @18:29:47.07 AFV.select :holdingCoil @18:29:47.18 delay 1 second @18:29:48.21 AS.reconfigure ASfast @18:29:48.27 AS.seek 0ml @18:30:15.64 AFV.select :carrierSolution @18:30:15.75 delay 2 seconds @18:30:17.78 AS.reconfigure ASconfig @18:30:17.83 AS.seek 1ml @18:31:15.58 delay 1 second @18:31:16.60 AFV.select :holdingCoil @18:31:16.71 delay 1 second @18:31:17.74 AS.reconfigure ASfast @18:31:17.79 AS.seek 0ml @18:31:45.19 AFV.select :carrierSolution @18:31:45.30 delay 2 seconds @18:31:47.33 AS.reconfigure ASconfig @18:31:47.38 AS.seek 1ml @18:32:45.14 delay 1 second @18:32:46.16 AFV.select :holdingCoil @18:32:46.27 delay 1 second @18:32:47.30 AS.reconfigure ASfast @18:32:47.35 AS.seek 0ml @18:33:14.72 ARV.dial between carrier and air1 @18:33:15.23 ATV.dial :air3 @18:33:16.53 AS.reconfigure ASslow1 @18:33:16.59 AS.seek 0.01ml @18:33:18.94 delay 2 seconds @18:33:20.96 ATV.dial :bleach @18:33:21.58 AS.seek 0.81ml @18:36:02.72 delay 3 seconds @18:36:05.74 ATV.dial :air3 @18:36:06.30 delay 3 seconds @18:36:09.37 AS.seek 0.835ml @18:36:14.68 delay 3 seconds @18:36:17.70 ATV.dial :bleach @18:36:18.33 AS.seek 0.82ml @18:36:21.67 delay 3 seconds @18:36:24.69 ATV.dial PCR @18:36:25.09 AS.reconfigure ASconfig @18:36:25.15 AS.seek :empty @18:37:12.59 PCR.program :bleachProfile @18:37:12.64 PCR program should complete in < 2:45 @18:37:12.65 PCR.run 10:00 @18:40:11.45 PCR.off @18:40:11.48 AFV.select :carrierSolution @18:40:11.60 delay 2 seconds @18:40:13.63 AS.seek 1ml @18:41:11.47 delay 1 second @18:41:12.49 AFV.select :holdingCoil @18:41:12.59 delay 1 second @18:41:13.62 AS.reconfigure ASfast @18:41:13.68 AS.seek 0ml @18:41:41.28 delay 3 seconds @18:41:44.30 AFV.select :carrierSolution @18:41:44.41 delay 2 seconds @18:41:46.44 AS.reconfigure ASconfig @18:41:46.50 AS.seek 1ml @18:42:44.27 delay 1 second @18:42:45.29 AFV.select :holdingCoil @18:42:45.40 delay 1 second @18:42:46.43 AS.reconfigure ASfast @18:42:46.48 AS.seek 0ml @18:43:13.84 delay 3 seconds @18:43:16.86 AFV.select :carrierSolution @18:43:16.96 delay 2 seconds @18:43:18.99 AS.reconfigure ASconfig @18:43:19.04 AS.seek 1ml @18:44:16.80 delay 1 second @18:44:17.82 AFV.select :holdingCoil @18:44:17.93 delay 1 second @18:44:18.96 AS.reconfigure ASfast @18:44:19.01 AS.seek 0ml @18:44:46.38 delay 3 seconds @18:44:49.40 AFV.select :carrierSolution @18:44:49.50 delay 2 seconds @18:44:51.53 AS.reconfigure ASconfig @18:44:51.59 AS.seek 1ml @18:45:49.36 delay 1 second @18:45:50.38 AFV.select :holdingCoil @18:45:50.49 delay 1 second @18:45:51.52 AS.reconfigure ASfast @18:45:51.57 AS.seek 0ml @18:46:19.05 delay 3 seconds @18:46:22.07 AFV.select :carrierSolution @18:46:22.17 delay 2 seconds @18:46:24.20 AS.reconfigure ASconfig @18:46:24.26 AS.seek 1ml @18:47:22.03 delay 1 second @18:47:23.05 AFV.select :holdingCoil @18:47:23.16 delay 1 second @18:47:24.19 AS.reconfigure ASnorm @18:47:24.24 AS.seek 0ml @18:48:14.96
AFV.select :carrierSolution @18:48:15.07 delay 2 seconds @18:48:17.10 AS.reconfigure ASconfig @18:48:17.15 AS.seek 0.5ml @18:48:46.24 delay 1 second @18:48:47.26 AFV.select :holdingCoil @18:48:47.37 ATV.dial :air1 @18:48:48.87 AS.reconfigure ASslow2 @18:48:48.93 AS.seek 0.51ml @18:48:59.20 delay 5 seconds @18:49:04.22 ATV.dial :extSrc @18:49:05.95 AS.seek 0.53ml @18:49:26.22 delay 10 seconds @18:49:36.24 ATV.dial :air1 @18:49:37.96 AS.reconfigure ASslow1 @18:49:38.02 AS.seek 0.56ml @18:49:44.31 ATV.dial :waste @18:49:44.88 AS.reconfigure ASconfig @18:49:44.94 AS.seek :empty @18:50:18.04 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118dec1818h.pcr @18:50:18.06 Building PCR Slug from extSrc with MM2 mix, PP4 primer @18:50:18.09 Priming with carrier @18:50:18.11 ATV.dial ARV @18:50:19.63 ARV.dial :carrier @18:50:19.98 AS.reconfigure ASnorm @18:50:20.04 AS.seek 0.499ml @18:50:45.69 AFV.select :carrierSolution @18:50:45.81 delay 2 seconds @18:50:47.84 AS.reconfigure ASconfig @18:50:47.90 AS.seek 1ml @18:51:16.97 delay 1 second @18:51:17.99 AFV.select :holdingCoil @18:51:18.09 delay 1 second @18:51:19.11 ATV.dial :waste @18:51:20.80 AS.reconfigure ASnorm @18:51:20.87 AS.seek :empty @18:52:11.63 AFV.select :carrierSolution @18:52:11.75 delay 2 seconds @18:52:13.78 AS.reconfigure ASconfig @18:52:13.83 AS.seek 0.5ml @18:52:42.94 delay 1 second @18:52:43.96 AFV.select :holdingCoil @18:52:44.06 Building Slug @18:52:44.07 ATV.dial :ARV @18:52:45.74 delay 2 seconds @18:52:47.76 ARV.dial :air1 @18:52:48.18 delay 2 seconds @18:52:50.25 AS.reconfigure ASslow2 @18:52:50.31 AS.seek 0.506ml @18:52:56.50 delay 2 seconds @18:52:58.52 ARV.dial :MM2 @18:53:00.05 delay 2 seconds @18:53:02.07 Adding MM2 @18:53:02.15 AS.seek 0.524ml @18:53:20.41 delay 25 seconds @18:53:45.43 ARV.dial :PP4 @18:53:45.83 Adding PP4 @18:53:45.84 delay 2 seconds @18:53:47.92 AS.seek 0.53ml @18:53:54.17 delay 10 seconds @18:54:04.20 ARV.dial :air3 @18:54:04.61 adding ARV air @18:54:04.62 delay 2 seconds @18:54:06.70 AS.seek 0.536ml @18:54:12.92 delay 5 seconds @18:54:17.94 ARV.dial :carrier @18:54:19.22 delay 7 seconds @18:54:26.30 AS.seek 0.594ml @18:55:24.86 delay 5 seconds @18:55:29.88 ATV.dial :extSrc @18:55:30.46 delay 2 seconds @18:55:32.53 AS.seek 0.6ml @18:55:38.72 delay 4 seconds @18:55:42.74 ATV.dial ARV @18:55:43.39 delay 2 seconds @18:55:45.41 ARV.dial :carrier @18:55:45.42 delay 2 seconds @18:55:47.50 AS.seek 0.65ml @18:56:37.92 delay 4 seconds @18:56:41.94 ATV.dial PCR @18:56:42.71 ASV.dial :waste1 @18:56:42.72 ARV.dial :air1 @18:56:43.14 Positioning Slug by 0.324ml @18:56:43.23 AS.seek 0.326ml @19:02:09.31 delay 5 seconds @19:02:14.33 ATV.dial ARV @19:02:15.13 ASV.dial :PCRlock @19:02:15.53 delay 5 seconds @19:02:20.55 Running Tt1 Profile60C300 thermocycling @19:02:20.56 PCR.program Profile60C300 @19:02:20.70 Profile60C300 should complete in < 1:45:21 @19:02:20.71 PCR.run 2:16:41.25 @20:24:08.54 PCR.off @20:24:10.05 PCR for Tt1 completed -- closed /var/log/esp/Tt118dec1818h.pcr @20:24:10.13 Cleaning PCR @20:24:10.14 AFV.select :carrierSolution @20:24:10.26 delay 2 seconds @20:24:12.29 AS.reconfigure ASconfig @20:24:12.34 AS.seek 0.5ml @20:24:22.66 delay 1 second @20:24:23.68 AFV.select :holdingCoil @20:24:23.78 ASV.dial :waste1 @20:24:24.18 ATV.dial :air3 @20:24:25.47 AS.reconfigure ASslow1 @20:24:25.53 AS.seek 0.51ml @20:24:27.83 delay 2 seconds @20:24:29.85 ATV.dial :bleach @20:24:30.47 AS.seek 0.81ml @20:25:31.09 delay 3 seconds @20:25:34.11 ATV.dial :air3 @20:25:34.68 delay 3 seconds @20:25:37.75 AS.seek 0.835ml @20:25:43.05 delay 3 seconds @20:25:46.07 ATV.dial :bleach @20:25:46.69 AS.seek 0.82ml @20:25:50.02 delay 3 seconds @20:25:53.04 ARV.dial :waste @20:25:53.66 delay 3 seconds @20:25:56.68 ATV.dial :ARV @20:25:57.57 AS.reconfigure ASconfig @20:25:57.63 AS.seek 0.47ml @20:26:18.02 AFV.select :carrierSolution @20:26:18.13 delay 2 seconds @20:26:20.16 AS.seek 1ml @20:26:50.99 delay 1 second @20:26:52.01 AFV.select :holdingCoil @20:26:52.11 delay 1 second @20:26:53.14 AS.reconfigure ASfast @20:26:53.19 AS.seek 0ml @20:27:20.55 AFV.select :carrierSolution @20:27:20.66 delay 2 seconds @20:27:22.69 AS.reconfigure ASconfig @20:27:22.74 AS.seek 1ml @20:28:20.48 delay 1 second @20:28:21.50 AFV.select :holdingCoil @20:28:21.60 delay 1 second @20:28:22.63 AS.reconfigure ASfast @20:28:22.68 AS.seek 0ml @20:28:50.12 AFV.select :carrierSolution @20:28:50.23 delay 2 seconds @20:28:52.26 AS.reconfigure ASconfig @20:28:52.31 AS.seek 1ml @20:29:50.05 delay 1 second @20:29:51.07 AFV.select :holdingCoil @20:29:51.17 delay 1 second @20:29:52.20 AS.reconfigure ASfast @20:29:52.25 AS.seek 0ml @20:30:19.60 ARV.dial between carrier and air1 @20:30:20.12 ATV.dial :air3 @20:30:21.41 AS.reconfigure ASslow1 @20:30:21.46 AS.seek 0.01ml @20:30:23.76 delay 2 seconds @20:30:25.78 ATV.dial :bleach @20:30:26.40 AS.seek 0.81ml @20:33:07.54 delay 3 seconds @20:33:10.56 ATV.dial :air3 @20:33:11.12 delay 3 seconds @20:33:14.57 AS.seek 0.835ml @20:33:19.87 delay 3 seconds @20:33:22.90 ATV.dial :bleach @20:33:23.52 AS.seek 0.82ml @20:33:26.77 delay 3 seconds @20:33:29.79 ATV.dial PCR @20:33:30.20 AS.reconfigure ASconfig @20:33:30.26 AS.seek :empty @20:34:17.71 PCR.program :bleachProfile @20:34:17.77 PCR program should complete in < 2:45 @20:34:17.78 PCR.run 10:00 @20:37:16.58 PCR.off @20:37:16.61 AFV.select :carrierSolution @20:37:16.73 delay 2 seconds @20:37:18.76 AS.seek 1ml @20:38:16.60 delay 1 second @20:38:17.62 AFV.select :holdingCoil @20:38:17.72 delay 1 second @20:38:18.75 AS.reconfigure ASfast @20:38:18.80 AS.seek 0ml @20:38:46.26 delay 3 seconds @20:38:49.28 AFV.select :carrierSolution @20:38:49.38 delay 2 seconds @20:38:51.41 AS.reconfigure ASconfig @20:38:51.47 AS.seek 1ml @20:39:49.27 delay 1 second @20:39:50.29 AFV.select :holdingCoil @20:39:50.39 delay 1 second @20:39:51.42 AS.reconfigure ASfast @20:39:51.47 AS.seek 0ml @20:40:18.85 delay 3 seconds @20:40:21.87 AFV.select :carrierSolution @20:40:21.97 delay 2 seconds @20:40:24.00 AS.reconfigure ASconfig @20:40:24.05 AS.seek 1ml @20:41:21.82 delay 1 second @20:41:22.84 AFV.select :holdingCoil @20:41:22.94 delay 1 second @20:41:23.97 AS.reconfigure ASfast @20:41:24.02 AS.seek 0ml @20:41:51.38 delay 3 seconds @20:41:54.40 AFV.select :carrierSolution @20:41:54.50 delay 2 seconds @20:41:56.53 AS.reconfigure ASconfig @20:41:56.59 AS.seek 1ml @20:42:54.34 delay 1 second @20:42:55.36 AFV.select :holdingCoil @20:42:55.46 delay 1 second @20:42:56.49 AS.reconfigure ASfast @20:42:56.54 AS.seek 0ml @20:43:23.88 delay 3 seconds @20:43:26.90 AFV.select :carrierSolution @20:43:27.00 delay 2 seconds @20:43:29.03 AS.reconfigure ASconfig @20:43:29.08 AS.seek 1ml @20:44:26.84 delay 1 second @20:44:27.86 AFV.select :holdingCoil @20:44:27.96 delay 1 second @20:44:28.99 AS.reconfigure ASnorm @20:44:29.04 AS.seek 0ml @20:45:19.75
AFV.select :carrierSolution @20:45:19.87 delay 2 seconds @20:45:21.90 AS.reconfigure ASconfig @20:45:21.95 AS.seek 0.5ml @20:45:51.02 delay 1 second @20:45:52.04 AFV.select :holdingCoil @20:45:52.14 ATV.dial :air1 @20:45:53.65 AS.reconfigure ASslow2 @20:45:53.70 AS.seek 0.506ml @20:46:00.01 ATV.dial :extSrc @20:46:01.71 AS.seek 0.512ml @20:46:08.12 delay 5 seconds @20:46:13.14 ATV.dial :air1 @20:46:14.86 AS.reconfigure ASslow1 @20:46:14.92 AS.seek 0.542ml @20:46:21.21 ATV.dial :waste @20:46:21.77 AS.reconfigure ASconfig @20:46:21.83 AS.seek :empty @20:46:53.51 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118dec1820h.pcr @20:46:53.53 Building PCR Slug from extSrc with MM2 mix, PP4 primer @20:46:53.56 Priming with carrier @20:46:53.58 ATV.dial ARV @20:46:55.07 ARV.dial :carrier @20:46:55.43 AS.reconfigure ASnorm @20:46:55.49 AS.seek 0.499ml @20:47:21.07 AFV.select :carrierSolution @20:47:21.18 delay 2 seconds @20:47:23.21 AS.reconfigure ASconfig @20:47:23.27 AS.seek 1ml @20:47:52.35 delay 1 second @20:47:53.37 AFV.select :holdingCoil @20:47:53.47 delay 1 second @20:47:54.49 ATV.dial :waste @20:47:56.18 AS.reconfigure ASnorm @20:47:56.24 AS.seek :empty @20:48:47.01 AFV.select :carrierSolution @20:48:47.12 delay 2 seconds @20:48:49.15 AS.reconfigure ASconfig @20:48:49.20 AS.seek 0.5ml @20:49:18.32 delay 1 second @20:49:19.34 AFV.select :holdingCoil @20:49:19.44 Building Slug @20:49:19.45 ATV.dial :ARV @20:49:21.49 delay 2 seconds @20:49:23.51 ARV.dial :air1 @20:49:23.90 delay 2 seconds @20:49:25.97 AS.reconfigure ASslow2 @20:49:26.03 AS.seek 0.506ml @20:49:32.32 delay 2 seconds @20:49:34.34 ARV.dial :MM2 @20:49:35.87 delay 2 seconds @20:49:37.89 Adding MM2 @20:49:38.01 AS.seek 0.524ml @20:49:56.29 delay 25 seconds @20:50:21.31 ARV.dial :PP4 @20:50:21.71 Adding PP4 @20:50:21.72 delay 2 seconds @20:50:23.80 AS.seek 0.53ml @20:50:30.21 delay 10 seconds @20:50:40.24 ARV.dial :air3 @20:50:40.63 adding ARV air @20:50:40.64 delay 2 seconds @20:50:42.72 AS.seek 0.536ml @20:50:49.09 delay 5 seconds @20:50:54.11 ARV.dial :carrier @20:50:55.42 delay 7 seconds @20:51:02.50 AS.seek 0.594ml @20:52:01.02 delay 5 seconds @20:52:06.04 ATV.dial :extSrc @20:52:06.62 delay 2 seconds @20:52:08.69 AS.seek 0.6ml @20:52:14.92 delay 4 seconds @20:52:18.94 ATV.dial ARV @20:52:19.51 delay 2 seconds @20:52:21.53 ARV.dial :carrier @20:52:21.54 delay 2 seconds @20:52:23.62 AS.seek 0.65ml @20:53:14.03 delay 4 seconds @20:53:18.05 ATV.dial PCR @20:53:18.84 ASV.dial :waste1 @20:53:18.85 ARV.dial :air1 @20:53:19.29 Positioning Slug by 0.324ml @20:53:19.38 AS.seek 0.326ml @20:58:45.38 delay 5 seconds @20:58:50.40 ATV.dial ARV @20:58:51.20 ASV.dial :PCRlock @20:58:51.60 delay 5 seconds @20:58:56.62 Running Tt1 Profile60C300 thermocycling @20:58:56.63 PCR.program Profile60C300 @20:58:56.78 Profile60C300 should complete in < 1:45:21 @20:58:56.79 PCR.run 2:16:41.25 @22:20:39.22 PCR.off @22:20:40.80 PCR for Tt1 completed -- closed /var/log/esp/Tt118dec1820h.pcr @22:20:40.88 Cleaning PCR @22:20:40.89 AFV.select :carrierSolution @22:20:41.01 delay 2 seconds @22:20:43.04 AS.reconfigure ASconfig @22:20:43.09 AS.seek 0.5ml @22:20:53.39 delay 1 second @22:20:54.41 AFV.select :holdingCoil @22:20:54.51 ASV.dial :waste1 @22:20:54.91 ATV.dial :air3 @22:20:56.22 AS.reconfigure ASslow1 @22:20:56.28 AS.seek 0.51ml @22:20:58.57 delay 2 seconds @22:21:00.59 ATV.dial :bleach @22:21:01.23 AS.seek 0.81ml @22:22:01.81 delay 3 seconds @22:22:04.83 ATV.dial :air3 @22:22:05.39 delay 3 seconds @22:22:08.46 AS.seek 0.835ml @22:22:13.78 delay 3 seconds @22:22:16.80 ATV.dial :bleach @22:22:17.42 AS.seek 0.82ml @22:22:20.75 delay 3 seconds @22:22:23.77 ARV.dial :waste @22:22:24.39 delay 3 seconds @22:22:27.41 ATV.dial :ARV @22:22:28.30 AS.reconfigure ASconfig @22:22:28.37 AS.seek 0.47ml @22:22:48.77 AFV.select :carrierSolution @22:22:48.88 delay 2 seconds @22:22:50.91 AS.seek 1ml @22:23:21.67 delay 1 second @22:23:22.69 AFV.select :holdingCoil @22:23:22.79 delay 1 second @22:23:23.82 AS.reconfigure ASfast @22:23:23.87 AS.seek 0ml @22:23:51.25 AFV.select :carrierSolution @22:23:51.36 delay 2 seconds @22:23:53.39 AS.reconfigure ASconfig @22:23:53.44 AS.seek 1ml @22:24:51.20 delay 1 second @22:24:52.22 AFV.select :holdingCoil @22:24:52.32 delay 1 second @22:24:53.35 AS.reconfigure ASfast @22:24:53.40 AS.seek 0ml @22:25:20.94 AFV.select :carrierSolution @22:25:21.05 delay 2 seconds @22:25:23.08 AS.reconfigure ASconfig @22:25:23.13 AS.seek 1ml @22:26:20.89 delay 1 second @22:26:21.91 AFV.select :holdingCoil @22:26:22.01 delay 1 second @22:26:23.04 AS.reconfigure ASfast @22:26:23.09 AS.seek 0ml @22:26:50.45 ARV.dial between carrier and air1 @22:26:50.97 ATV.dial :air3 @22:26:52.28 AS.reconfigure ASslow1 @22:26:52.34 AS.seek 0.01ml @22:26:54.63 delay 2 seconds @22:26:56.65 ATV.dial :bleach @22:26:57.26 AS.seek 0.81ml @22:29:38.39 delay 3 seconds @22:29:41.41 ATV.dial :air3 @22:29:41.97 delay 3 seconds @22:29:45.04 AS.seek 0.835ml @22:29:50.35 delay 3 seconds @22:29:53.37 ATV.dial :bleach @22:29:54.00 AS.seek 0.82ml @22:29:57.30 delay 3 seconds @22:30:00.32 ATV.dial PCR @22:30:00.73 AS.reconfigure ASconfig @22:30:00.79 AS.seek :empty @22:30:48.25 PCR.program :bleachProfile @22:30:48.30 PCR program should complete in < 2:45 @22:30:48.31 PCR.run 10:00 @22:33:47.13 PCR.off @22:33:47.17 AFV.select :carrierSolution @22:33:47.28 delay 2 seconds @22:33:49.31 AS.seek 1ml @22:34:47.19 delay 1 second @22:34:48.21 AFV.select :holdingCoil @22:34:48.31 delay 1 second @22:34:49.34 AS.reconfigure ASfast @22:34:49.39 AS.seek 0ml @22:35:17.27 delay 3 seconds @22:35:20.29 AFV.select :carrierSolution @22:35:20.39 delay 2 seconds @22:35:22.42 AS.reconfigure ASconfig @22:35:22.48 AS.seek 1ml @22:36:20.25 delay 1 second @22:36:21.27 AFV.select :holdingCoil @22:36:21.37 delay 1 second @22:36:22.40 AS.reconfigure ASfast @22:36:22.45 AS.seek 0ml @22:36:49.91 delay 3 seconds @22:36:52.93 AFV.select :carrierSolution @22:36:53.03 delay 2 seconds @22:36:55.06 AS.reconfigure ASconfig @22:36:55.11 AS.seek 1ml @22:37:52.86 delay 1 second @22:37:53.88 AFV.select :holdingCoil @22:37:53.98 delay 1 second @22:37:55.01 AS.reconfigure ASfast @22:37:55.06 AS.seek 0ml @22:38:22.52 delay 3 seconds @22:38:25.54 AFV.select :carrierSolution @22:38:25.64 delay 2 seconds @22:38:27.67 AS.reconfigure ASconfig @22:38:27.73 AS.seek 1ml @22:39:25.50 delay 1 second @22:39:26.52 AFV.select :holdingCoil @22:39:26.62 delay 1 second @22:39:27.65 AS.reconfigure ASfast @22:39:27.70 AS.seek 0ml @22:39:55.06 delay 3 seconds @22:39:58.08 AFV.select :carrierSolution @22:39:58.18 delay 2 seconds @22:40:00.21 AS.reconfigure ASconfig @22:40:00.27 AS.seek 1ml @22:40:58.06 delay 1 second @22:40:59.08 AFV.select :holdingCoil @22:40:59.18 delay 1 second @22:41:00.21 AS.reconfigure ASnorm @22:41:00.26 AS.seek 0ml @22:41:51.00
AFV.select :carrierSolution @22:41:51.12 delay 2 seconds @22:41:53.15 AS.reconfigure ASconfig @22:41:53.20 AS.seek 0.5ml @22:42:22.22 delay 1 second @22:42:23.24 AFV.select :holdingCoil @22:42:23.34 ATV.dial :air1 @22:42:24.84 AS.reconfigure ASslow1 @22:42:24.90 AS.seek 0.52ml @22:42:29.17 delay 5 seconds @22:42:34.19 ATV.dial :extSrc @22:42:35.92 AS.reconfigure ASslow2 @22:42:35.98 AS.seek 0.545ml @22:43:01.33 delay 10 seconds @22:43:11.36 ATV.dial :air1 @22:43:13.09 AS.reconfigure ASslow1 @22:43:13.14 AS.seek 0.575ml @22:43:19.52 ATV.dial :waste @22:43:20.08 AS.reconfigure ASconfig @22:43:20.14 AS.seek :empty @22:43:53.70 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118dec1822h.pcr @22:43:53.73 Building PCR Slug from extSrc with MM2 mix, PP4 primer @22:43:53.75 Priming with carrier @22:43:53.77 ATV.dial ARV @22:43:55.28 ARV.dial :carrier @22:43:55.64 AS.reconfigure ASnorm @22:43:55.70 AS.seek 0.499ml @22:44:21.27 AFV.select :carrierSolution @22:44:21.38 delay 2 seconds @22:44:23.41 AS.reconfigure ASconfig @22:44:23.47 AS.seek 1ml @22:44:52.58 delay 1 second @22:44:53.60 AFV.select :holdingCoil @22:44:53.70 delay 1 second @22:44:54.72 ATV.dial :waste @22:44:56.41 AS.reconfigure ASnorm @22:44:56.47 AS.seek :empty @22:45:47.24 AFV.select :carrierSolution @22:45:47.35 delay 2 seconds @22:45:49.38 AS.reconfigure ASconfig @22:45:49.43 AS.seek 0.5ml @22:46:18.56 delay 1 second @22:46:19.58 AFV.select :holdingCoil @22:46:19.68 Building Slug @22:46:19.69 ATV.dial :ARV @22:46:21.35 delay 2 seconds @22:46:23.37 ARV.dial :air1 @22:46:23.89 delay 2 seconds @22:46:25.96 AS.reconfigure ASslow2 @22:46:26.03 AS.seek 0.506ml @22:46:32.38 delay 2 seconds @22:46:34.40 ARV.dial :MM2 @22:46:35.94 delay 2 seconds @22:46:37.96 Adding MM2 @22:46:38.04 AS.seek 0.524ml @22:46:56.52 delay 25 seconds @22:47:21.54 ARV.dial :PP4 @22:47:21.95 Adding PP4 @22:47:21.96 delay 2 seconds @22:47:24.04 AS.seek 0.53ml @22:47:30.33 delay 10 seconds @22:47:40.36 ARV.dial :air3 @22:47:40.77 adding ARV air @22:47:40.78 delay 2 seconds @22:47:42.86 AS.seek 0.536ml @22:47:49.05 delay 5 seconds @22:47:54.07 ARV.dial :carrier @22:47:55.38 delay 7 seconds @22:48:02.46 AS.seek 0.594ml @22:49:00.95 delay 5 seconds @22:49:06.36 ATV.dial :extSrc @22:49:06.92 delay 2 seconds @22:49:08.99 AS.seek 0.6ml @22:49:15.25 delay 4 seconds @22:49:19.27 ATV.dial ARV @22:49:19.83 delay 2 seconds @22:49:21.85 ARV.dial :carrier @22:49:21.86 delay 2 seconds @22:49:23.94 AS.seek 0.65ml @22:50:14.42 delay 4 seconds @22:50:18.44 ATV.dial PCR @22:50:19.22 ASV.dial :waste1 @22:50:19.23 ARV.dial :air1 @22:50:19.65 Positioning Slug by 0.324ml @22:50:19.74 AS.seek 0.326ml @22:55:46.05 delay 5 seconds @22:55:51.07 ATV.dial ARV @22:55:51.86 ASV.dial :PCRlock @22:55:52.27 delay 5 seconds @22:55:57.29 Running Tt1 Profile60C300 thermocycling @22:55:57.30 PCR.program Profile60C300 @22:55:57.45 Profile60C300 should complete in < 1:45:21 @22:55:57.46 PCR.run 2:16:41.25 @00:17:39.22CET19-Dec-18 PCR.off @00:17:40.72 PCR for Tt1 completed -- closed /var/log/esp/Tt118dec1822h.pcr @00:17:40.79 Cleaning PCR @00:17:40.80 AFV.select :carrierSolution @00:17:40.92 delay 2 seconds @00:17:42.95 AS.reconfigure ASconfig @00:17:43.00 AS.seek 0.5ml @00:17:53.29 delay 1 second @00:17:54.31 AFV.select :holdingCoil @00:17:54.41 ASV.dial :waste1 @00:17:54.80 ATV.dial :air3 @00:17:56.10 AS.reconfigure ASslow1 @00:17:56.16 AS.seek 0.51ml @00:17:58.46 delay 2 seconds @00:18:00.48 ATV.dial :bleach @00:18:01.11 AS.seek 0.81ml @00:19:01.77 delay 3 seconds @00:19:04.79 ATV.dial :air3 @00:19:05.35 delay 3 seconds @00:19:08.42 AS.seek 0.835ml @00:19:13.74 delay 3 seconds @00:19:16.76 ATV.dial :bleach @00:19:17.38 AS.seek 0.82ml @00:19:20.68 delay 3 seconds @00:19:23.70 ARV.dial :waste @00:19:24.32 delay 3 seconds @00:19:27.34 ATV.dial :ARV @00:19:28.24 AS.reconfigure ASconfig @00:19:28.30 AS.seek 0.47ml @00:19:48.70 AFV.select :carrierSolution @00:19:48.81 delay 2 seconds @00:19:50.84 AS.seek 1ml @00:20:21.62 delay 1 second @00:20:22.64 AFV.select :holdingCoil @00:20:22.74 delay 1 second @00:20:23.77 AS.reconfigure ASfast @00:20:23.82 AS.seek 0ml @00:20:51.24 AFV.select :carrierSolution @00:20:51.35 delay 2 seconds @00:20:53.38 AS.reconfigure ASconfig @00:20:53.43 AS.seek 1ml @00:21:51.20 delay 1 second @00:21:52.22 AFV.select :holdingCoil @00:21:52.32 delay 1 second @00:21:53.35 AS.reconfigure ASfast @00:21:53.40 AS.seek 0ml @00:22:21.18 AFV.select :carrierSolution @00:22:21.30 delay 2 seconds @00:22:23.33 AS.reconfigure ASconfig @00:22:23.38 AS.seek 1ml @00:23:21.15 delay 1 second @00:23:22.17 AFV.select :holdingCoil @00:23:22.27 delay 1 second @00:23:23.30 AS.reconfigure ASfast @00:23:23.35 AS.seek 0ml @00:23:50.88 ARV.dial between carrier and air1 @00:23:51.38 ATV.dial :air3 @00:23:52.68 AS.reconfigure ASslow1 @00:23:52.74 AS.seek 0.01ml @00:23:55.09 delay 2 seconds @00:23:57.11 ATV.dial :bleach @00:23:57.72 AS.seek 0.81ml @00:26:38.93 delay 3 seconds @00:26:41.95 ATV.dial :air3 @00:26:42.51 delay 3 seconds @00:26:45.58 AS.seek 0.835ml @00:26:50.88 delay 3 seconds @00:26:53.91 ATV.dial :bleach @00:26:54.54 AS.seek 0.82ml @00:26:57.87 delay 3 seconds @00:27:00.89 ATV.dial PCR @00:27:01.29 AS.reconfigure ASconfig @00:27:01.35 AS.seek :empty @00:27:48.82 PCR.program :bleachProfile @00:27:48.87 PCR program should complete in < 2:45 @00:27:48.88 PCR.run 10:00 @00:30:47.69 PCR.off @00:30:47.72 AFV.select :carrierSolution @00:30:47.83 delay 2 seconds @00:30:49.86 AS.seek 1ml @00:31:47.68 delay 1 second @00:31:48.70 AFV.select :holdingCoil @00:31:48.80 delay 1 second @00:31:49.83 AS.reconfigure ASfast @00:31:49.88 AS.seek 0ml @00:32:17.21 delay 3 seconds @00:32:20.23 AFV.select :carrierSolution @00:32:20.33 delay 2 seconds @00:32:22.36 AS.reconfigure ASconfig @00:32:22.42 AS.seek 1ml @00:33:20.20 delay 1 second @00:33:21.22 AFV.select :holdingCoil @00:33:21.32 delay 1 second @00:33:22.35 AS.reconfigure ASfast @00:33:22.40 AS.seek 0ml @00:33:49.76 delay 3 seconds @00:33:52.78 AFV.select :carrierSolution @00:33:52.88 delay 2 seconds @00:33:54.91 AS.reconfigure ASconfig @00:33:54.97 AS.seek 1ml @00:34:52.74 delay 1 second @00:34:53.76 AFV.select :holdingCoil @00:34:53.86 delay 1 second @00:34:54.89 AS.reconfigure ASfast @00:34:54.94 AS.seek 0ml @00:35:22.31 delay 3 seconds @00:35:25.33 AFV.select :carrierSolution @00:35:25.43 delay 2 seconds @00:35:27.46 AS.reconfigure ASconfig @00:35:27.52 AS.seek 1ml @00:36:25.27 delay 1 second @00:36:26.29 AFV.select :holdingCoil @00:36:26.39 delay 1 second @00:36:27.42 AS.reconfigure ASfast @00:36:27.47 AS.seek 0ml @00:36:54.81 delay 3 seconds @00:36:57.83 AFV.select :carrierSolution @00:36:57.93 delay 2 seconds @00:37:00.29 AS.reconfigure ASconfig @00:37:00.41 AS.seek 1ml @00:37:58.20 delay 1 second @00:37:59.22 AFV.select :holdingCoil @00:37:59.32 delay 1 second @00:38:00.35 AS.reconfigure ASnorm @00:38:00.40 AS.seek 0ml @00:38:51.11
AFV.select :carrierSolution @00:38:51.23 delay 2 seconds @00:38:53.26 AS.reconfigure ASconfig @00:38:53.31 AS.seek 0.5ml @00:39:22.37 delay 1 second @00:39:23.39 AFV.select :holdingCoil @00:39:23.49 ATV.dial :air1 @00:39:25.01 AS.reconfigure ASslow2 @00:39:25.07 AS.seek 0.506ml @00:39:31.37 ATV.dial :extSrc @00:39:33.07 AS.seek 0.512ml @00:39:39.54 delay 5 seconds @00:39:44.56 ATV.dial :air1 @00:39:46.30 AS.reconfigure ASslow1 @00:39:46.35 AS.seek 0.542ml @00:39:52.67 ATV.dial :waste @00:39:53.23 AS.reconfigure ASconfig @00:39:53.29 AS.seek :empty @00:40:24.96 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118dec1900h.pcr @00:40:24.98 Building PCR Slug from extSrc with MM2 mix, PP4 primer @00:40:25.01 Priming with carrier @00:40:25.02 ATV.dial ARV @00:40:26.51 ARV.dial :carrier @00:40:26.87 AS.reconfigure ASnorm @00:40:26.93 AS.seek 0.499ml @00:40:52.54 AFV.select :carrierSolution @00:40:52.65 delay 2 seconds @00:40:54.68 AS.reconfigure ASconfig @00:40:54.74 AS.seek 1ml @00:41:23.81 delay 1 second @00:41:24.83 AFV.select :holdingCoil @00:41:24.93 delay 1 second @00:41:25.95 ATV.dial :waste @00:41:27.63 AS.reconfigure ASnorm @00:41:27.69 AS.seek :empty @00:42:18.46 AFV.select :carrierSolution @00:42:18.58 delay 2 seconds @00:42:20.61 AS.reconfigure ASconfig @00:42:20.67 AS.seek 0.5ml @00:42:49.81 delay 1 second @00:42:50.83 AFV.select :holdingCoil @00:42:50.93 Building Slug @00:42:50.94 ATV.dial :ARV @00:42:52.70 delay 2 seconds @00:42:54.72 ARV.dial :air1 @00:42:55.13 delay 2 seconds @00:42:57.20 AS.reconfigure ASslow2 @00:42:57.26 AS.seek 0.506ml @00:43:03.51 delay 2 seconds @00:43:05.53 ARV.dial :MM2 @00:43:07.04 delay 2 seconds @00:43:09.06 Adding MM2 @00:43:09.14 AS.seek 0.524ml @00:43:27.45 delay 25 seconds @00:43:52.47 ARV.dial :PP4 @00:43:52.88 Adding PP4 @00:43:52.89 delay 2 seconds @00:43:54.97 AS.seek 0.53ml @00:44:01.20 delay 10 seconds @00:44:11.23 ARV.dial :air3 @00:44:11.63 adding ARV air @00:44:11.64 delay 2 seconds @00:44:13.72 AS.seek 0.536ml @00:44:19.96 delay 5 seconds @00:44:24.98 ARV.dial :carrier @00:44:26.29 delay 7 seconds @00:44:33.37 AS.seek 0.594ml @00:45:31.85 delay 5 seconds @00:45:36.87 ATV.dial :extSrc @00:45:37.43 delay 2 seconds @00:45:39.50 AS.seek 0.6ml @00:45:45.71 delay 4 seconds @00:45:49.73 ATV.dial ARV @00:45:50.38 delay 2 seconds @00:45:52.40 ARV.dial :carrier @00:45:52.41 delay 2 seconds @00:45:54.49 AS.seek 0.65ml @00:46:44.92 delay 4 seconds @00:46:48.94 ATV.dial PCR @00:46:49.73 ASV.dial :waste1 @00:46:49.74 ARV.dial :air1 @00:46:50.15 Positioning Slug by 0.324ml @00:46:50.24 AS.seek 0.326ml @00:52:16.35 delay 5 seconds @00:52:21.37 ATV.dial ARV @00:52:22.17 ASV.dial :PCRlock @00:52:22.57 delay 5 seconds @00:52:27.98 Running Tt1 Profile60C300 thermocycling @00:52:28.00 PCR.program Profile60C300 @00:52:28.14 Profile60C300 should complete in < 1:45:21 @00:52:28.15 PCR.run 2:16:41.25 @02:14:11.45 PCR.off @02:14:12.95 PCR for Tt1 completed -- closed /var/log/esp/Tt118dec1900h.pcr @02:14:13.02 Cleaning PCR @02:14:13.04 AFV.select :carrierSolution @02:14:13.16 delay 2 seconds @02:14:15.19 AS.reconfigure ASconfig @02:14:15.24 AS.seek 0.5ml @02:14:25.58 delay 1 second @02:14:26.60 AFV.select :holdingCoil @02:14:26.70 ASV.dial :waste1 @02:14:27.09 ATV.dial :air3 @02:14:28.40 AS.reconfigure ASslow1 @02:14:28.46 AS.seek 0.51ml @02:14:30.76 delay 2 seconds @02:14:32.78 ATV.dial :bleach @02:14:33.42 AS.seek 0.81ml @02:15:34.02 delay 3 seconds @02:15:37.04 ATV.dial :air3 @02:15:37.59 delay 3 seconds @02:15:40.66 AS.seek 0.835ml @02:15:45.95 delay 3 seconds @02:15:48.97 ATV.dial :bleach @02:15:49.59 AS.seek 0.82ml @02:15:52.89 delay 3 seconds @02:15:55.91 ARV.dial :waste @02:15:56.53 delay 3 seconds @02:15:59.55 ATV.dial :ARV @02:16:00.44 AS.reconfigure ASconfig @02:16:00.50 AS.seek 0.47ml @02:16:20.92 AFV.select :carrierSolution @02:16:21.03 delay 2 seconds @02:16:23.06 AS.seek 1ml @02:16:53.86 delay 1 second @02:16:54.88 AFV.select :holdingCoil @02:16:54.99 delay 1 second @02:16:56.02 AS.reconfigure ASfast @02:16:56.07 AS.seek 0ml @02:17:23.44 AFV.select :carrierSolution @02:17:23.55 delay 2 seconds @02:17:25.58 AS.reconfigure ASconfig @02:17:25.63 AS.seek 1ml @02:18:23.80 delay 1 second @02:18:24.82 AFV.select :holdingCoil @02:18:24.92 delay 1 second @02:18:25.95 AS.reconfigure ASfast @02:18:26.00 AS.seek 0ml @02:18:53.36 AFV.select :carrierSolution @02:18:53.47 delay 2 seconds @02:18:55.50 AS.reconfigure ASconfig @02:18:55.55 AS.seek 1ml @02:19:53.31 delay 1 second @02:19:54.33 AFV.select :holdingCoil @02:19:54.43 delay 1 second @02:19:55.46 AS.reconfigure ASfast @02:19:55.51 AS.seek 0ml @02:20:22.86 ARV.dial between carrier and air1 @02:20:23.36 ATV.dial :air3 @02:20:24.67 AS.reconfigure ASslow1 @02:20:24.73 AS.seek 0.01ml @02:20:27.05 delay 2 seconds @02:20:29.07 ATV.dial :bleach @02:20:29.72 AS.seek 0.81ml @02:23:10.86 delay 3 seconds @02:23:13.88 ATV.dial :air3 @02:23:14.45 delay 3 seconds @02:23:17.52 AS.seek 0.835ml @02:23:22.84 delay 3 seconds @02:23:25.86 ATV.dial :bleach @02:23:26.50 AS.seek 0.82ml @02:23:29.83 delay 3 seconds @02:23:32.85 ATV.dial PCR @02:23:33.25 AS.reconfigure ASconfig @02:23:33.31 AS.seek :empty @02:24:20.78 PCR.program :bleachProfile @02:24:20.84 PCR program should complete in < 2:45 @02:24:20.85 PCR.run 10:00 @02:27:19.65 PCR.off @02:27:19.68 AFV.select :carrierSolution @02:27:19.79 delay 2 seconds @02:27:21.82 AS.seek 1ml @02:28:19.61 delay 1 second @02:28:20.63 AFV.select :holdingCoil @02:28:20.73 delay 1 second @02:28:21.76 AS.reconfigure ASfast @02:28:21.81 AS.seek 0ml @02:28:49.16 delay 3 seconds @02:28:52.18 AFV.select :carrierSolution @02:28:52.28 delay 2 seconds @02:28:54.31 AS.reconfigure ASconfig @02:28:54.37 AS.seek 1ml @02:29:52.13 delay 1 second @02:29:53.15 AFV.select :holdingCoil @02:29:53.25 delay 1 second @02:29:54.28 AS.reconfigure ASfast @02:29:54.33 AS.seek 0ml @02:30:21.69 delay 3 seconds @02:30:24.71 AFV.select :carrierSolution @02:30:24.81 delay 2 seconds @02:30:26.84 AS.reconfigure ASconfig @02:30:26.90 AS.seek 1ml @02:31:24.67 delay 1 second @02:31:25.69 AFV.select :holdingCoil @02:31:25.79 delay 1 second @02:31:26.82 AS.reconfigure ASfast @02:31:26.87 AS.seek 0ml @02:31:54.33 delay 3 seconds @02:31:57.35 AFV.select :carrierSolution @02:31:57.45 delay 2 seconds @02:31:59.48 AS.reconfigure ASconfig @02:31:59.54 AS.seek 1ml @02:32:57.31 delay 1 second @02:32:58.33 AFV.select :holdingCoil @02:32:58.43 delay 1 second @02:32:59.46 AS.reconfigure ASfast @02:32:59.51 AS.seek 0ml @02:33:26.89 delay 3 seconds @02:33:29.91 AFV.select :carrierSolution @02:33:30.01 delay 2 seconds @02:33:32.04 AS.reconfigure ASconfig @02:33:32.09 AS.seek 1ml @02:34:29.85 delay 1 second @02:34:30.87 AFV.select :holdingCoil @02:34:30.97 delay 1 second @02:34:32.00 AS.reconfigure ASnorm @02:34:32.05 AS.seek 0ml @02:35:22.76
AFV.select :carrierSolution @02:35:22.87 delay 2 seconds @02:35:24.90 AS.reconfigure ASconfig @02:35:24.95 AS.seek 0.5ml @02:35:54.00 delay 1 second @02:35:55.02 AFV.select :holdingCoil @02:35:55.12 ATV.dial :air1 @02:35:56.62 AS.reconfigure ASslow1 @02:35:56.68 AS.seek 0.52ml @02:36:00.99 delay 5 seconds @02:36:06.01 ATV.dial :extSrc @02:36:07.71 AS.reconfigure ASslow2 @02:36:07.77 AS.seek 0.538ml @02:36:26.13 delay 10 seconds @02:36:36.15 ATV.dial :air1 @02:36:37.88 AS.reconfigure ASslow1 @02:36:37.94 AS.seek 0.568ml @02:36:44.25 ATV.dial :waste @02:36:44.81 AS.reconfigure ASconfig @02:36:45.18 AS.seek :empty @02:37:18.41 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118dec1902h.pcr @02:37:18.43 Building PCR Slug from extSrc with MM2 mix, PP4 primer @02:37:18.46 Priming with carrier @02:37:18.48 ATV.dial ARV @02:37:20.08 ARV.dial :carrier @02:37:20.44 AS.reconfigure ASnorm @02:37:20.50 AS.seek 0.499ml @02:37:46.10 AFV.select :carrierSolution @02:37:46.22 delay 2 seconds @02:37:48.25 AS.reconfigure ASconfig @02:37:48.31 AS.seek 1ml @02:38:17.38 delay 1 second @02:38:18.40 AFV.select :holdingCoil @02:38:18.50 delay 1 second @02:38:19.52 ATV.dial :waste @02:38:21.19 AS.reconfigure ASnorm @02:38:21.25 AS.seek :empty @02:39:12.00 AFV.select :carrierSolution @02:39:12.11 delay 2 seconds @02:39:14.14 AS.reconfigure ASconfig @02:39:14.20 AS.seek 0.5ml @02:39:43.33 delay 1 second @02:39:44.35 AFV.select :holdingCoil @02:39:44.45 Building Slug @02:39:44.46 ATV.dial :ARV @02:39:46.11 delay 2 seconds @02:39:48.13 ARV.dial :air1 @02:39:48.56 delay 2 seconds @02:39:50.64 AS.reconfigure ASslow2 @02:39:50.70 AS.seek 0.506ml @02:39:56.89 delay 2 seconds @02:39:58.91 ARV.dial :MM2 @02:40:00.44 delay 2 seconds @02:40:02.46 Adding MM2 @02:40:02.54 AS.seek 0.524ml @02:40:20.83 delay 25 seconds @02:40:45.85 ARV.dial :PP4 @02:40:46.25 Adding PP4 @02:40:46.26 delay 2 seconds @02:40:48.34 AS.seek 0.53ml @02:40:54.64 delay 10 seconds @02:41:04.67 ARV.dial :air3 @02:41:05.06 adding ARV air @02:41:05.07 delay 2 seconds @02:41:07.15 AS.seek 0.536ml @02:41:13.78 delay 5 seconds @02:41:18.80 ARV.dial :carrier @02:41:20.11 delay 7 seconds @02:41:27.19 AS.seek 0.594ml @02:42:25.89 delay 5 seconds @02:42:30.91 ATV.dial :extSrc @02:42:31.49 delay 2 seconds @02:42:33.56 AS.seek 0.6ml @02:42:39.77 delay 4 seconds @02:42:43.79 ATV.dial ARV @02:42:44.36 delay 2 seconds @02:42:46.38 ARV.dial :carrier @02:42:46.39 delay 2 seconds @02:42:48.47 AS.seek 0.65ml @02:43:39.03 delay 4 seconds @02:43:43.05 ATV.dial PCR @02:43:43.82 ASV.dial :waste1 @02:43:43.83 ARV.dial :air1 @02:43:44.26 Positioning Slug by 0.324ml @02:43:44.35 AS.seek 0.326ml @02:49:10.22 delay 5 seconds @02:49:15.24 ATV.dial ARV @02:49:16.03 ASV.dial :PCRlock @02:49:16.44 delay 5 seconds @02:49:21.46 Running Tt1 Profile60C300 thermocycling @02:49:21.47 PCR.program Profile60C300 @02:49:21.62 Profile60C300 should complete in < 1:45:21 @02:49:21.63 PCR.run 2:16:41.25 @03:57:58.31 Session Ended @04:11:03.35 PCR.off @04:11:04.85 PCR for Tt1 completed -- closed /var/log/esp/Tt118dec1902h.pcr @04:11:04.91 Cleaning PCR @04:11:04.92 AFV.select :carrierSolution @04:11:05.02 delay 2 seconds @04:11:07.05 AS.reconfigure ASconfig @04:11:07.09 AS.seek 0.5ml @04:11:17.40 delay 1 second @04:11:18.42 AFV.select :holdingCoil @04:11:18.52 ASV.dial :waste1 @04:11:18.92 ATV.dial :air3 @04:11:20.21 AS.reconfigure ASslow1 @04:11:20.25 AS.seek 0.51ml @04:11:22.52 delay 2 seconds @04:11:24.54 ATV.dial :bleach @04:11:25.16 AS.seek 0.81ml @04:12:25.79 delay 3 seconds @04:12:28.81 ATV.dial :air3 @04:12:29.35 delay 3 seconds @04:12:32.42 AS.seek 0.835ml @04:12:37.71 delay 3 seconds @04:12:40.73 ATV.dial :bleach @04:12:41.33 AS.seek 0.82ml @04:12:44.76 delay 3 seconds @04:12:47.78 ARV.dial :waste @04:12:48.40 delay 3 seconds @04:12:51.42 ATV.dial :ARV @04:12:52.29 AS.reconfigure ASconfig @04:12:52.34 AS.seek 0.47ml @04:13:12.76 AFV.select :carrierSolution @04:13:12.86 delay 2 seconds @04:13:14.89 AS.seek 1ml @04:13:45.63 delay 1 second @04:13:46.65 AFV.select :holdingCoil @04:13:46.75 delay 1 second @04:13:47.78 AS.reconfigure ASfast @04:13:47.82 AS.seek 0ml @04:14:15.17 AFV.select :carrierSolution @04:14:15.27 delay 2 seconds @04:14:17.30 AS.reconfigure ASconfig @04:14:17.34 AS.seek 1ml @04:15:15.10 delay 1 second @04:15:16.12 AFV.select :holdingCoil @04:15:16.22 delay 1 second @04:15:17.25 AS.reconfigure ASfast @04:15:17.29 AS.seek 0ml @04:15:44.71 AFV.select :carrierSolution @04:15:44.81 delay 2 seconds @04:15:46.84 AS.reconfigure ASconfig @04:15:46.88 AS.seek 1ml @04:16:44.63 delay 1 second @04:16:45.65 AFV.select :holdingCoil @04:16:45.75 delay 1 second @04:16:46.78 AS.reconfigure ASfast @04:16:46.82 AS.seek 0ml @04:17:14.17 ARV.dial between carrier and air1 @04:17:14.67 ATV.dial :air3 @04:17:15.94 AS.reconfigure ASslow1 @04:17:15.98 AS.seek 0.01ml @04:17:18.27 delay 2 seconds @04:17:20.29 ATV.dial :bleach @04:17:20.90 AS.seek 0.81ml @04:20:02.02 delay 3 seconds @04:20:05.04 ATV.dial :air3 @04:20:05.91 delay 3 seconds @04:20:09.04 AS.seek 0.835ml @04:20:14.33 delay 3 seconds @04:20:17.35 ATV.dial :bleach @04:20:17.96 AS.seek 0.82ml @04:20:21.21 delay 3 seconds @04:20:24.23 ATV.dial PCR @04:20:24.62 AS.reconfigure ASconfig @04:20:24.66 AS.seek :empty @04:21:12.10 PCR.program :bleachProfile @04:21:12.15 PCR program should complete in < 2:45 @04:21:12.16 PCR.run 10:00 @04:24:10.95 PCR.off @04:24:10.98 AFV.select :carrierSolution @04:24:11.08 delay 2 seconds @04:24:13.11 AS.seek 1ml @04:25:10.95 delay 1 second @04:25:11.97 AFV.select :holdingCoil @04:25:12.07 delay 1 second @04:25:13.10 AS.reconfigure ASfast @04:25:13.14 AS.seek 0ml @04:25:40.51 delay 3 seconds @04:25:43.53 AFV.select :carrierSolution @04:25:43.63 delay 2 seconds @04:25:45.66 AS.reconfigure ASconfig @04:25:45.71 AS.seek 1ml @04:26:43.46 delay 1 second @04:26:44.48 AFV.select :holdingCoil @04:26:44.58 delay 1 second @04:26:45.61 AS.reconfigure ASfast @04:26:45.65 AS.seek 0ml @04:27:12.99 delay 3 seconds @04:27:16.01 AFV.select :carrierSolution @04:27:16.11 delay 2 seconds @04:27:18.14 AS.reconfigure ASconfig @04:27:18.18 AS.seek 1ml @04:28:15.92 delay 1 second @04:28:16.94 AFV.select :holdingCoil @04:28:17.04 delay 1 second @04:28:18.07 AS.reconfigure ASfast @04:28:18.11 AS.seek 0ml @04:28:45.46 delay 3 seconds @04:28:48.48 AFV.select :carrierSolution @04:28:48.58 delay 2 seconds @04:28:50.61 AS.reconfigure ASconfig @04:28:50.66 AS.seek 1ml @04:29:48.42 delay 1 second @04:29:49.44 AFV.select :holdingCoil @04:29:49.54 delay 1 second @04:29:50.57 AS.reconfigure ASfast @04:29:50.61 AS.seek 0ml @04:30:17.95 delay 3 seconds @04:30:20.97 AFV.select :carrierSolution @04:30:21.07 delay 2 seconds @04:30:23.10 AS.reconfigure ASconfig @04:30:23.14 AS.seek 1ml @04:31:20.90 delay 1 second @04:31:21.92 AFV.select :holdingCoil @04:31:22.02 delay 1 second @04:31:23.05 AS.reconfigure ASnorm @04:31:23.09 AS.seek 0ml @04:32:13.79
AFV.select :carrierSolution @04:32:13.89 delay 2 seconds @04:32:15.92 AS.reconfigure ASconfig @04:32:15.96 AS.seek 0.5ml @04:32:45.03 delay 1 second @04:32:46.05 AFV.select :holdingCoil @04:32:46.15 ATV.dial :air1 @04:32:47.64 AS.reconfigure ASslow2 @04:32:47.69 AS.seek 0.506ml @04:32:54.17 ATV.dial :extSrc @04:32:55.87 AS.seek 0.512ml @04:33:02.15 delay 5 seconds @04:33:07.17 ATV.dial :air1 @04:33:08.89 AS.reconfigure ASslow1 @04:33:08.94 AS.seek 0.542ml @04:33:15.28 ATV.dial :waste @04:33:15.82 AS.reconfigure ASconfig @04:33:15.86 AS.seek :empty @04:33:47.53 Setting up PCR for Tt1 -- writing /var/log/esp/Tt118dec1904h.pcr @04:33:47.55 Building PCR Slug from extSrc with MM2 mix, PP4 primer @04:33:47.56 Priming with carrier @04:33:47.57 ATV.dial ARV @04:33:49.06 ARV.dial :carrier @04:33:49.40 AS.reconfigure ASnorm @04:33:49.44 AS.seek 0.499ml @04:34:15.01 AFV.select :carrierSolution @04:34:15.11 delay 2 seconds @04:34:17.14 AS.reconfigure ASconfig @04:34:17.18 AS.seek 1ml @04:34:46.24 delay 1 second @04:34:47.26 AFV.select :holdingCoil @04:34:47.36 delay 1 second @04:34:48.38 ATV.dial :waste @04:34:50.06 AS.reconfigure ASnorm @04:34:50.10 AS.seek :empty @04:35:40.82 AFV.select :carrierSolution @04:35:40.92 delay 2 seconds @04:35:42.95 AS.reconfigure ASconfig @04:35:42.99 AS.seek 0.5ml @04:36:12.09 delay 1 second @04:36:13.11 AFV.select :holdingCoil @04:36:13.21 Building Slug @04:36:13.22 ATV.dial :ARV @04:36:15.24 delay 2 seconds @04:36:17.26 ARV.dial :air1 @04:36:17.67 delay 2 seconds @04:36:19.74 AS.reconfigure ASslow2 @04:36:19.79 AS.seek 0.506ml @04:36:25.96 delay 2 seconds @04:36:27.98 ARV.dial :MM2 @04:36:29.48 delay 2 seconds @04:36:31.50 Adding MM2 @04:36:31.57 AS.seek 0.524ml @04:36:49.85 delay 25 seconds @04:37:14.87 ARV.dial :PP4 @04:37:15.26 Adding PP4 @04:37:15.27 delay 2 seconds @04:37:17.34 AS.seek 0.53ml @04:37:23.54 delay 10 seconds @04:37:33.57 ARV.dial :air3 @04:37:33.96 adding ARV air @04:37:33.97 delay 2 seconds @04:37:36.04 AS.seek 0.536ml @04:37:42.28 delay 5 seconds @04:37:47.30 ARV.dial :carrier @04:37:48.57 delay 7 seconds @04:37:55.65 AS.seek 0.594ml @04:38:54.14 delay 5 seconds @04:38:59.16 ATV.dial :extSrc @04:38:59.73 delay 2 seconds @04:39:01.80 AS.seek 0.6ml @04:39:08.04 delay 4 seconds @04:39:12.06 ATV.dial ARV @04:39:12.60 delay 2 seconds @04:39:14.62 ARV.dial :carrier @04:39:14.63 delay 2 seconds @04:39:16.70 AS.seek 0.65ml @04:40:07.10 delay 4 seconds @04:40:11.12 ATV.dial PCR @04:40:11.89 ASV.dial :waste1 @04:40:11.90 ARV.dial :air1 @04:40:12.30 Positioning Slug by 0.324ml @04:40:12.37 AS.seek 0.326ml @04:45:38.37 delay 5 seconds @04:45:43.39 ATV.dial ARV @04:45:44.17 ASV.dial :PCRlock @04:45:44.56 delay 5 seconds @04:45:49.58 Running Tt1 Profile60C300 thermocycling @04:45:49.59 PCR.program Profile60C300 @04:45:49.71 Profile60C300 should complete in < 1:45:21 @04:45:49.72 PCR.run 2:16:41.25 @06:07:29.98 PCR.off @06:07:31.57 PCR for Tt1 completed -- closed /var/log/esp/Tt118dec1904h.pcr @06:07:31.63 Cleaning PCR @06:07:31.64 AFV.select :carrierSolution @06:07:31.73 delay 2 seconds @06:07:33.76 AS.reconfigure ASconfig @06:07:33.80 AS.seek 0.5ml @06:07:44.10 delay 1 second @06:07:45.12 AFV.select :holdingCoil @06:07:45.22 ASV.dial :waste1 @06:07:45.61 ATV.dial :air3 @06:07:46.91 AS.reconfigure ASslow1 @06:07:46.95 AS.seek 0.51ml @06:07:49.23 delay 2 seconds @06:07:51.25 ATV.dial :bleach @06:07:51.87 AS.seek 0.81ml @06:08:52.45 delay 3 seconds @06:08:55.47 ATV.dial :air3 @06:08:56.02 delay 3 seconds @06:08:59.09 AS.seek 0.835ml @06:09:04.37 delay 3 seconds @06:09:07.39 ATV.dial :bleach @06:09:08.00 AS.seek 0.82ml @06:09:11.25 delay 3 seconds @06:09:14.27 ARV.dial :waste @06:09:14.88 delay 3 seconds @06:09:17.90 ATV.dial :ARV @06:09:18.77 AS.reconfigure ASconfig @06:09:18.82 AS.seek 0.47ml @06:09:39.21 AFV.select :carrierSolution @06:09:39.31 delay 2 seconds @06:09:41.34 AS.seek 1ml @06:10:12.10 delay 1 second @06:10:13.12 AFV.select :holdingCoil @06:10:13.22 delay 1 second @06:10:14.25 AS.reconfigure ASfast @06:10:14.29 AS.seek 0ml @06:10:41.75 AFV.select :carrierSolution @06:10:41.85 delay 2 seconds @06:10:43.88 AS.reconfigure ASconfig @06:10:43.92 AS.seek 1ml @06:11:41.71 delay 1 second @06:11:42.73 AFV.select :holdingCoil @06:11:42.83 delay 1 second @06:11:43.86 AS.reconfigure ASfast @06:11:43.90 AS.seek 0ml @06:12:11.27 AFV.select :carrierSolution @06:12:11.37 delay 2 seconds @06:12:13.40 AS.reconfigure ASconfig @06:12:13.44 AS.seek 1ml @06:13:11.22 delay 1 second @06:13:12.24 AFV.select :holdingCoil @06:13:12.34 delay 1 second @06:13:13.37 AS.reconfigure ASfast @06:13:13.41 AS.seek 0ml @06:13:40.75 ARV.dial between carrier and air1 @06:13:41.24 ATV.dial :air3 @06:13:42.53 AS.reconfigure ASslow1 @06:13:42.57 AS.seek 0.01ml @06:13:44.85 delay 2 seconds @06:13:46.87 ATV.dial :bleach @06:13:47.50 AS.seek 0.81ml @06:16:28.63 delay 3 seconds @06:16:31.65 ATV.dial :air3 @06:16:32.19 delay 3 seconds @06:16:35.26 AS.seek 0.835ml @06:16:40.57 delay 3 seconds @06:16:43.59 ATV.dial :bleach @06:16:44.20 AS.seek 0.82ml @06:16:47.50 delay 3 seconds @06:16:50.52 ATV.dial PCR @06:16:50.91 AS.reconfigure ASconfig @06:16:50.95 AS.seek :empty @06:17:38.42 PCR.program :bleachProfile @06:17:38.47 PCR program should complete in < 2:45 @06:17:38.48 PCR.run 10:00 @06:20:37.28 PCR.off @06:20:37.30 AFV.select :carrierSolution @06:20:37.40 delay 2 seconds @06:20:39.43 AS.seek 1ml @06:21:37.25 delay 1 second @06:21:38.27 AFV.select :holdingCoil @06:21:38.37 delay 1 second @06:21:39.40 AS.reconfigure ASfast @06:21:39.44 AS.seek 0ml @06:22:06.78 delay 3 seconds @06:22:09.80 AFV.select :carrierSolution @06:22:10.29 delay 2 seconds @06:22:12.32 AS.reconfigure ASconfig @06:22:12.36 AS.seek 1ml @06:23:10.11 delay 1 second @06:23:11.13 AFV.select :holdingCoil @06:23:11.23 delay 1 second @06:23:12.26 AS.reconfigure ASfast @06:23:12.30 AS.seek 0ml @06:23:39.64 delay 3 seconds @06:23:42.66 AFV.select :carrierSolution @06:23:42.76 delay 2 seconds @06:23:44.79 AS.reconfigure ASconfig @06:23:44.87 AS.seek 1ml @06:24:42.60 delay 1 second @06:24:43.62 AFV.select :holdingCoil @06:24:43.72 delay 1 second @06:24:44.75 AS.reconfigure ASfast @06:24:44.79 AS.seek 0ml @06:25:12.13 delay 3 seconds @06:25:15.15 AFV.select :carrierSolution @06:25:15.25 delay 2 seconds @06:25:17.28 AS.reconfigure ASconfig @06:25:17.32 AS.seek 1ml @06:26:15.11 delay 1 second @06:26:16.13 AFV.select :holdingCoil @06:26:16.23 delay 1 second @06:26:17.26 AS.reconfigure ASfast @06:26:17.30 AS.seek 0ml @06:26:44.63 delay 3 seconds @06:26:47.65 AFV.select :carrierSolution @06:26:47.75 delay 2 seconds @06:26:49.78 AS.reconfigure ASconfig @06:26:49.83 AS.seek 1ml @06:27:47.58 delay 1 second @06:27:48.60 AFV.select :holdingCoil @06:27:48.70 delay 1 second @06:27:49.73 AS.reconfigure ASnorm @06:27:49.77 AS.seek 0ml @06:28:40.51
deconEluateRecoveryLine extSrc ATV.dial :waste @06:28:41.75 AS.seek 0ml @06:28:41.81 ATV.dial :air3 @06:28:42.63 AS.reconfigure ASslow1 @06:28:42.67 AS.seek 0.01ml @06:28:45.00 delay 2 seconds @06:28:47.02 ATV.dial :bleach @06:28:47.47 AS.seek 0.31ml @06:29:48.10 delay 3 seconds @06:29:51.12 ATV.dial :air3 @06:29:51.66 delay 3 seconds @06:29:54.73 AS.seek 0.335ml @06:30:00.10 delay 3 seconds @06:30:03.12 ATV.dial :bleach @06:30:03.72 AS.seek 0.32ml @06:30:06.99 delay 3 seconds @06:30:10.01 ATV.dial :extSrc @06:30:10.62 AS.seek 0ml @06:31:15.19 AFV.select :carrierSolution @06:31:15.29 delay 2 seconds @06:31:17.32 AS.reconfigure ASconfig @06:31:17.36 AS.seek 0.5ml @06:31:46.39 delay 1 second @06:31:47.41 AFV.select :holdingCoil @06:31:47.51 ATV.dial :extSrc @06:31:47.53 AS.reconfigure ASslow1 @06:31:47.57 AS.seek 0ml @06:33:28.38 AFV.select :carrierSolution @06:33:28.48 delay 2 seconds @06:33:30.51 AS.reconfigure ASconfig @06:33:30.55 AS.seek 0.5ml @06:33:59.58 delay 1 second @06:34:00.60 AFV.select :holdingCoil @06:34:00.70 ATV.dial :extSrc @06:34:00.72 AS.reconfigure ASslow1 @06:34:00.76 AS.seek 0ml @06:35:41.58 AFV.select :carrierSolution @06:35:41.68 delay 2 seconds @06:35:43.71 AS.reconfigure ASconfig @06:35:43.75 AS.seek 0.5ml @06:36:12.79 delay 1 second @06:36:13.81 AFV.select :holdingCoil @06:36:13.91 ATV.dial :extSrc @06:36:13.93 AS.reconfigure ASslow1 @06:36:13.97 AS.seek 0ml @06:37:54.77 AFV.select :carrierSolution @06:37:54.87 delay 2 seconds @06:37:56.90 AS.reconfigure ASconfig @06:37:56.94 AS.seek 0.5ml @06:38:25.97 delay 1 second @06:38:26.99 AFV.select :holdingCoil @06:38:27.09 ATV.dial :extSrc @06:38:27.11 AS.reconfigure ASslow1 @06:38:27.15 AS.seek 0ml @06:40:07.97 ATV.dial :air2 @06:40:09.29 AS.reconfigure ASslow0 @06:40:09.34 AS.seek 0.5ml @06:40:59.94 ATV.dial :extSrc @06:41:01.42 AS.seek 0.02ml @06:41:50.00 delay 1 second @06:41:51.02 ATV.dial :waste @06:41:52.30 AS.reconfigure ASconfig @06:41:52.34 AS.seek :empty @06:41:53.83 deconEluateRecoveryLine extSrc @06:41:53.84 ATV.dial :waste @06:41:53.85 AS.reconfigure ASnorm @06:41:53.89 AS.seek 0ml @06:41:54.24 ATV.dial :air3 @06:41:55.07 AS.reconfigure ASslow1 @06:41:55.11 AS.seek 0.01ml @06:41:57.41 delay 2 seconds @06:41:59.43 ATV.dial :bleach @06:41:59.88 AS.seek 0.31ml @06:43:00.46 delay 3 seconds @06:43:03.48 ATV.dial :air3 @06:43:04.02 delay 3 seconds @06:43:07.09 AS.seek 0.335ml @06:43:12.39 delay 3 seconds @06:43:15.41 ATV.dial :bleach @06:43:16.39 AS.seek 0.32ml @06:43:19.69 delay 3 seconds @06:43:22.71 ATV.dial :extSrc @06:43:23.33 AS.seek 0ml @06:44:27.93 AFV.select :carrierSolution @06:44:28.03 delay 2 seconds @06:44:30.06 AS.reconfigure ASconfig @06:44:30.10 AS.seek 0.5ml @06:44:59.13 delay 1 second @06:45:00.15 AFV.select :holdingCoil @06:45:00.25 ATV.dial :extSrc @06:45:00.28 AS.reconfigure ASslow1 @06:45:00.32 AS.seek 0ml @06:46:41.13 AFV.select :carrierSolution @06:46:41.23 delay 2 seconds @06:46:43.26 AS.reconfigure ASconfig @06:46:43.30 AS.seek 0.5ml @06:47:12.32 delay 1 second @06:47:13.34 AFV.select :holdingCoil @06:47:13.44 ATV.dial :extSrc @06:47:13.46 AS.reconfigure ASslow1 @06:47:13.50 AS.seek 0ml @06:48:54.32 AFV.select :carrierSolution @06:48:54.42 delay 2 seconds @06:48:56.45 AS.reconfigure ASconfig @06:48:56.49 AS.seek 0.5ml @06:49:25.52 delay 1 second @06:49:26.54 AFV.select :holdingCoil @06:49:26.64 ATV.dial :extSrc @06:49:26.66 AS.reconfigure ASslow1 @06:49:26.70 AS.seek 0ml @06:51:07.50 AFV.select :carrierSolution @06:51:07.60 delay 2 seconds @06:51:09.63 AS.reconfigure ASconfig @06:51:09.67 AS.seek 0.5ml @06:51:38.71 delay 1 second @06:51:39.73 AFV.select :holdingCoil @06:51:39.83 ATV.dial :extSrc @06:51:39.85 AS.reconfigure ASslow1 @06:51:39.89 AS.seek 0ml @06:53:20.69 ATV.dial :air2 @06:53:22.02 AS.reconfigure ASslow0 @06:53:22.06 AS.seek 0.5ml @06:54:12.65 ATV.dial :extSrc @06:54:14.13 AS.seek 0.02ml @06:55:02.71 delay 1 second @06:55:03.73 ATV.dial :waste @06:55:05.01 AS.reconfigure ASconfig @06:55:05.05 AS.seek :empty @06:55:06.55 deconEluateRecoveryLine extSrc @06:55:06.56 ATV.dial :waste @06:55:06.57 AS.reconfigure ASnorm @06:55:06.61 AS.seek 0ml @06:55:07.02 ATV.dial :air3 @06:55:07.86 AS.reconfigure ASslow1 @06:55:07.90 AS.seek 0.01ml @06:55:10.16 delay 2 seconds @06:55:12.18 ATV.dial :bleach @06:55:12.63 AS.seek 0.31ml @06:56:13.18 delay 3 seconds @06:56:16.20 ATV.dial :air3 @06:56:16.74 delay 3 seconds @06:56:19.81 AS.seek 0.335ml @06:56:25.02 delay 3 seconds @06:56:28.04 ATV.dial :bleach @06:56:28.64 AS.seek 0.32ml @06:56:31.90 delay 3 seconds @06:56:34.92 ATV.dial :extSrc @06:56:35.54 AS.seek 0ml @06:57:40.11 AFV.select :carrierSolution @06:57:40.21 delay 2 seconds @06:57:42.24 AS.reconfigure ASconfig @06:57:42.28 AS.seek 0.5ml @06:58:11.32 delay 1 second @06:58:12.34 AFV.select :holdingCoil @06:58:12.44 ATV.dial :extSrc @06:58:12.46 AS.reconfigure ASslow1 @06:58:12.50 AS.seek 0ml @06:59:53.32 AFV.select :carrierSolution @06:59:53.42 delay 2 seconds @06:59:55.45 AS.reconfigure ASconfig @06:59:55.49 AS.seek 0.5ml @07:00:24.52 delay 1 second @07:00:25.54 AFV.select :holdingCoil @07:00:25.64 ATV.dial :extSrc @07:00:25.66 AS.reconfigure ASslow1 @07:00:25.70 AS.seek 0ml @07:02:06.51 AFV.select :carrierSolution @07:02:06.61 delay 2 seconds @07:02:08.64 AS.reconfigure ASconfig @07:02:08.68 AS.seek 0.5ml @07:02:37.69 delay 1 second @07:02:38.71 AFV.select :holdingCoil @07:02:38.81 ATV.dial :extSrc @07:02:38.83 AS.reconfigure ASslow1 @07:02:38.87 AS.seek 0ml @07:04:19.68 AFV.select :carrierSolution @07:04:19.78 delay 2 seconds @07:04:21.81 AS.reconfigure ASconfig @07:04:21.85 AS.seek 0.5ml @07:04:50.88 delay 1 second @07:04:51.90 AFV.select :holdingCoil @07:04:52.00 ATV.dial :extSrc @07:04:52.02 AS.reconfigure ASslow1 @07:04:52.06 AS.seek 0ml @07:06:32.87 ATV.dial :air2 @07:06:34.19 AS.reconfigure ASslow0 @07:06:34.23 AS.seek 0.5ml @07:07:24.85 ATV.dial :extSrc @07:07:26.35 AS.seek 0.02ml @07:08:14.88 delay 1 second @07:08:15.90 ATV.dial :waste @07:08:17.18 AS.reconfigure ASconfig @07:08:17.22 AS.seek :empty @17:00:09.68CEST06-May-19
ESP2 v3.30 I2C Gateway v3.06 for core bus @17:00:11.36 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @17:00:11.61 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @17:00:16.24 Polling Can every 10:00 @17:00:18.41 SPE.coast @17:00:18.46 SPE.configure SPEconfig @17:00:18.54 AS.reconfigure ASconfig @17:00:18.58 AS.hold @17:00:18.63 AS.configure ASconfig @17:00:18.72 SS.configure SSconfig @17:00:18.80 CC.configure CCconfig @17:00:18.88 CS.configure CSconfig @17:00:18.96 PC.configure PCconfig @17:00:19.04 PS.configure PSconfig @17:00:19.14 Forearm.configure LooseForearm @17:00:19.23 Elbow.configure LooseElbow @17:00:19.71 SC.configure SCloose @17:00:19.88 SE.configure SEconfig @17:00:19.96 CH.coast @17:00:20.05 CH.configure CHconfig @17:00:20.10 PH.coast @17:00:20.20 PH.configure PHconfig @17:00:20.28 PCR.off @17:00:20.32 PCR.pidtemp= 50,0.1,0,300,25,25 @17:00:20.36 PCR.caltemp= 2.8814,0.0372,0 @17:00:20.40 All dwarf controllers running firmware version 3.75 @17:00:20.58 Cleanly exited at 16:53:45CEST06-May-19 @17:00:20.61 Loading /home/esp/esp2/mission/stdMERwithoutESPready.rb mission @17:00:20.63 AFV.select :carrierSolution @17:00:20.73 delay 2 seconds @17:00:22.76 AS.seek 0.5ml @17:00:23.57 Session Begun @17:00:35.28 -> showlog 10 @17:00:51.83
delay 1 second @17:00:52.85 AFV.select :holdingCoil @17:00:52.95 ATV.dial :extSrc @17:00:54.44 AS.reconfigure ASslow1 @17:00:54.50 AS.seek 0.53ml @17:01:00.79 delay 5 seconds @17:01:05.88 AS.reconfigure ASslow2 @17:01:05.94 AS.seek 0.54ml @17:01:16.18 delay 20 seconds @17:01:36.26 AS.reconfigure ASslow1 @17:01:36.32 AS.seek 0.57ml @17:01:42.60 delay 5 seconds @17:01:47.68 AS.reconfigure ASslow2 @17:01:47.74 AS.seek 0.58ml @17:01:57.99 delay 20 seconds @17:02:18.07 AS.reconfigure ASslow1 @17:02:18.13 AS.seek 0.61ml @17:02:24.38 delay 5 seconds @17:02:29.47 AS.reconfigure ASslow2 @17:02:29.53 AS.seek 0.62ml @17:02:39.85 delay 20 seconds @17:02:59.93 AS.reconfigure ASslow1 @17:02:59.99 AS.seek 0.65ml @17:03:06.27 delay 5 seconds @17:03:11.36 AS.reconfigure ASslow2 @17:03:11.42 AS.seek 0.66ml @17:03:21.68 delay 20 seconds @17:03:41.71 AS.reconfigure ASslow1 @17:03:41.77 AS.seek 0.705ml @17:03:51.10 delay 10 seconds @17:04:01.13 ATV.dial :air1 @17:04:02.85 AS.seek 0.725ml @17:04:07.13 delay 3 seconds @17:04:10.16 ATV.dial :waste @17:04:10.72 AS.reconfigure ASslow0 @17:04:10.78 AS.seek :empty @17:05:24.21 Setting up PCR for Ss -- writing /var/log/esp/Ss19may0617h.pcr @17:05:24.24 Building PCR Slug from extSrc with MM2 mix, PP4 primer @17:05:24.26 Priming with carrier @17:05:24.28 ATV.dial ARV @17:05:25.77 ARV.dial :carrier @17:05:26.13 AS.reconfigure ASnorm @17:05:26.20 AS.seek 0.499ml @17:05:51.77 AFV.select :carrierSolution @17:05:51.89 delay 2 seconds @17:05:53.91 AS.reconfigure ASconfig @17:05:53.98 AS.seek 1ml @17:06:23.07 delay 1 second @17:06:24.10 AFV.select :holdingCoil @17:06:24.20 delay 1 second @17:06:25.22 ATV.dial :waste @17:06:26.89 AS.reconfigure ASnorm @17:06:26.95 AS.seek :empty @17:07:17.71 AFV.select :carrierSolution @17:07:17.83 delay 2 seconds @17:07:19.85 AS.reconfigure ASconfig @17:07:19.91 AS.seek 0.5ml @17:07:49.04 delay 1 second @17:07:50.07 AFV.select :holdingCoil @17:07:50.17 Building Slug @17:07:50.18 ATV.dial :ARV @17:07:51.85 delay 2 seconds @17:07:53.87 ARV.dial :air1 @17:07:54.29 delay 2 seconds @17:07:56.36 AS.reconfigure ASslow2 @17:07:56.42 AS.seek 0.506ml @17:08:02.80 delay 2 seconds @17:08:04.83 ARV.dial :MM2 @17:08:06.31 delay 2 seconds @17:08:08.33 Adding MM2 @17:08:08.42 AS.seek 0.524ml @17:08:26.91 delay 25 seconds @17:08:51.94 ARV.dial :PP4 @17:08:52.33 Adding PP4 @17:08:52.34 delay 2 seconds @17:08:54.42 AS.seek 0.53ml @17:09:00.66 delay 10 seconds @17:09:10.70 ARV.dial :air3 @17:09:11.20 adding ARV air @17:09:11.21 delay 2 seconds @17:09:13.29 AS.seek 0.536ml @17:09:19.72 delay 5 seconds @17:09:24.74 ARV.dial :carrier @17:09:26.02 delay 7 seconds @17:09:33.10 AS.seek 0.594ml @17:10:31.61 delay 5 seconds @17:10:36.63 ATV.dial :extSrc @17:10:37.19 delay 2 seconds @17:10:39.26 AS.seek 0.6ml @17:10:45.46 delay 4 seconds @17:10:49.49 ATV.dial ARV @17:10:50.05 delay 2 seconds @17:10:52.07 ARV.dial :carrier @17:10:52.08 delay 2 seconds @17:10:54.16 AS.seek 0.65ml @17:11:44.60 delay 4 seconds @17:11:48.63 ATV.dial PCR @17:11:49.41 ASV.dial :waste1 @17:11:49.50 ARV.dial :air1 @17:11:49.89 Positioning Slug by 0.324ml @17:11:49.98 AS.seek 0.326ml @17:17:16.10 delay 5 seconds @17:17:21.13 ATV.dial ARV @17:17:21.91 ASV.dial :PCRlock @17:17:22.31 delay 5 seconds @17:17:27.33 Running Ss Profile60C350 thermocycling @17:17:27.35 PCR.program Profile60C350 @17:17:27.50 Profile60C350 should complete in < 1:48:05 @17:17:27.51 PCR.run 2:20:06.25 @18:41:00.91 PCR.off @18:41:02.89 PCR for Ss completed -- closed /var/log/esp/Ss19may0617h.pcr @18:41:02.97 Cleaning PCR @18:41:02.98 AFV.select :carrierSolution @18:41:03.10 delay 2 seconds @18:41:05.12 AS.reconfigure ASconfig @18:41:05.18 AS.seek 0.5ml @18:41:15.46 delay 1 second @18:41:16.49 AFV.select :holdingCoil @18:41:16.59 ASV.dial :waste1 @18:41:16.98 ATV.dial :air3 @18:41:18.29 AS.reconfigure ASslow1 @18:41:18.36 AS.seek 0.51ml @18:41:20.63 delay 2 seconds @18:41:22.66 ATV.dial :bleach @18:41:23.27 AS.seek 0.81ml @18:42:23.94 delay 3 seconds @18:42:26.97 ATV.dial :air3 @18:42:27.53 delay 3 seconds @18:42:30.60 AS.seek 0.835ml @18:42:36.01 delay 3 seconds @18:42:39.04 ATV.dial :bleach @18:42:39.65 AS.seek 0.82ml @18:42:42.94 delay 3 seconds @18:42:45.97 ARV.dial :waste @18:42:46.58 delay 3 seconds @18:42:49.60 ATV.dial :ARV @18:42:50.60 AS.reconfigure ASconfig @18:42:50.66 AS.seek 0.47ml @18:43:11.05 AFV.select :carrierSolution @18:43:11.17 delay 2 seconds @18:43:13.19 AS.seek 1ml @18:43:43.98 delay 1 second @18:43:45.01 AFV.select :holdingCoil @18:43:45.11 delay 1 second @18:43:46.13 AS.reconfigure ASfast @18:43:46.19 AS.seek 0ml @18:44:13.94 AFV.select :carrierSolution @18:44:14.06 delay 2 seconds @18:44:16.08 AS.reconfigure ASconfig @18:44:16.14 AS.seek 1ml @18:45:13.90 delay 1 second @18:45:14.93 AFV.select :holdingCoil @18:45:15.03 delay 1 second @18:45:16.05 AS.reconfigure ASfast @18:45:16.11 AS.seek 0ml @18:45:43.70 AFV.select :carrierSolution @18:45:43.82 delay 2 seconds @18:45:45.84 AS.reconfigure ASconfig @18:45:45.90 AS.seek 1ml @18:46:43.69 delay 1 second @18:46:44.72 AFV.select :holdingCoil @18:46:44.82 delay 1 second @18:46:45.84 AS.reconfigure ASfast @18:46:45.90 AS.seek 0ml @18:47:13.38 ARV.dial between carrier and air1 @18:47:13.89 ATV.dial :air3 @18:47:15.18 AS.reconfigure ASslow1 @18:47:15.25 AS.seek 0.01ml @18:47:17.72 delay 2 seconds @18:47:19.74 ATV.dial :bleach @18:47:20.36 AS.seek 0.81ml @18:50:01.59 delay 3 seconds @18:50:04.62 ATV.dial :air3 @18:50:05.19 delay 3 seconds @18:50:08.26 AS.seek 0.835ml @18:50:13.56 delay 3 seconds @18:50:16.58 ATV.dial :bleach @18:50:17.19 AS.seek 0.82ml @18:50:20.52 delay 3 seconds @18:50:23.55 ATV.dial PCR @18:50:23.97 AS.reconfigure ASconfig @18:50:24.03 AS.seek :empty @18:51:11.51 PCR.program :bleachProfile @18:51:11.57 PCR program should complete in < 2:45 @18:51:11.58 PCR.run 10:00 @18:54:07.37 PCR.off @18:54:07.40 AFV.select :carrierSolution @18:54:07.51 delay 2 seconds @18:54:09.53 AS.seek 1ml @18:55:07.32 delay 1 second @18:55:08.35 AFV.select :holdingCoil @18:55:08.45 delay 1 second @18:55:09.47 AS.reconfigure ASfast @18:55:09.53 AS.seek 0ml @18:55:36.87 delay 3 seconds @18:55:39.90 AFV.select :carrierSolution @18:55:40.00 delay 2 seconds @18:55:42.02 AS.reconfigure ASconfig @18:55:42.08 AS.seek 1ml @18:56:39.88 delay 1 second @18:56:40.91 AFV.select :holdingCoil @18:56:41.02 delay 1 second @18:56:42.04 AS.reconfigure ASfast @18:56:42.10 AS.seek 0ml @18:57:09.46 delay 3 seconds @18:57:12.49 AFV.select :carrierSolution @18:57:12.59 delay 2 seconds @18:57:14.61 AS.reconfigure ASconfig @18:57:14.68 AS.seek 1ml @18:58:12.48 delay 1 second @18:58:13.51 AFV.select :holdingCoil @18:58:13.61 delay 1 second @18:58:14.63 AS.reconfigure ASfast @18:58:14.69 AS.seek 0ml @18:58:42.04 delay 3 seconds @18:58:45.07 AFV.select :carrierSolution @18:58:45.17 delay 2 seconds @18:58:47.19 AS.reconfigure ASconfig @18:58:47.26 AS.seek 1ml @18:59:45.05 delay 1 second @18:59:46.08 AFV.select :holdingCoil @18:59:46.18 delay 1 second @18:59:47.20 AS.reconfigure ASfast @18:59:47.26 AS.seek 0ml @19:00:14.63 delay 3 seconds @19:00:17.65 AFV.select :carrierSolution @19:00:17.76 delay 2 seconds @19:00:19.78 AS.reconfigure ASconfig @19:00:19.84 AS.seek 1ml @19:01:17.59 delay 1 second @19:01:18.62 AFV.select :holdingCoil @19:01:18.73 delay 1 second @19:01:19.75 AS.reconfigure ASnorm @19:01:19.81 AS.seek 0ml @19:02:10.50
AFV.select :carrierSolution @19:02:10.61 delay 2 seconds @19:02:12.63 AS.reconfigure ASconfig @19:02:12.69 AS.seek 0.5ml @19:02:41.74 delay 1 second @19:02:42.77 AFV.select :holdingCoil @19:02:42.87 ATV.dial :air1 @19:02:44.36 AS.reconfigure ASslow2 @19:02:44.42 AS.seek 0.506ml @19:02:50.88 ATV.dial :extSrc @19:02:52.60 AS.seek 0.512ml @19:02:58.99 delay 5 seconds @19:03:04.02 ATV.dial :air1 @19:03:05.76 AS.reconfigure ASslow1 @19:03:05.82 AS.seek 0.542ml @19:03:12.10 ATV.dial :waste @19:03:12.67 AS.reconfigure ASconfig @19:03:12.73 AS.seek :empty @19:03:44.38 Setting up PCR for Ss -- writing /var/log/esp/Ss19may0619h.pcr @19:03:44.41 Building PCR Slug from extSrc with MM2 mix, PP4 primer @19:03:44.43 Priming with carrier @19:03:44.45 ATV.dial ARV @19:03:45.96 ARV.dial :carrier @19:03:46.32 AS.reconfigure ASnorm @19:03:46.39 AS.seek 0.499ml @19:04:11.96 AFV.select :carrierSolution @19:04:12.08 delay 2 seconds @19:04:14.10 AS.reconfigure ASconfig @19:04:14.16 AS.seek 1ml @19:04:43.23 delay 1 second @19:04:44.26 AFV.select :holdingCoil @19:04:44.36 delay 1 second @19:04:45.38 ATV.dial :waste @19:04:47.06 AS.reconfigure ASnorm @19:04:47.12 AS.seek :empty @19:05:37.87 AFV.select :carrierSolution @19:05:38.00 delay 2 seconds @19:05:40.02 AS.reconfigure ASconfig @19:05:40.08 AS.seek 0.5ml @19:06:09.16 delay 1 second @19:06:10.19 AFV.select :holdingCoil @19:06:10.29 Building Slug @19:06:10.30 ATV.dial :ARV @19:06:11.99 delay 2 seconds @19:06:14.01 ARV.dial :air1 @19:06:14.43 delay 2 seconds @19:06:16.50 AS.reconfigure ASslow2 @19:06:16.56 AS.seek 0.506ml @19:06:23.49 delay 2 seconds @19:06:25.52 ARV.dial :MM2 @19:06:27.00 delay 2 seconds @19:06:29.02 Adding MM2 @19:06:29.10 AS.seek 0.524ml @19:06:47.37 delay 25 seconds @19:07:12.40 ARV.dial :PP4 @19:07:12.78 Adding PP4 @19:07:12.79 delay 2 seconds @19:07:14.87 AS.seek 0.53ml @19:07:21.30 delay 10 seconds @19:07:31.34 ARV.dial :air3 @19:07:31.72 adding ARV air @19:07:31.73 delay 2 seconds @19:07:33.81 AS.seek 0.536ml @19:07:40.16 delay 5 seconds @19:07:45.18 ARV.dial :carrier @19:07:46.46 delay 7 seconds @19:07:53.54 AS.seek 0.594ml @19:08:52.17 delay 5 seconds @19:08:57.19 ATV.dial :extSrc @19:08:57.75 delay 2 seconds @19:08:59.82 AS.seek 0.6ml @19:09:06.10 delay 4 seconds @19:09:10.13 ATV.dial ARV @19:09:10.69 delay 2 seconds @19:09:12.71 ARV.dial :carrier @19:09:12.72 delay 2 seconds @19:09:14.80 AS.seek 0.65ml @19:10:05.47 delay 4 seconds @19:10:09.49 ATV.dial PCR @19:10:10.26 ASV.dial :waste1 @19:10:10.28 ARV.dial :air1 @19:10:10.70 Positioning Slug by 0.324ml @19:10:10.79 AS.seek 0.326ml @19:15:36.82 delay 5 seconds @19:15:41.85 ATV.dial ARV @19:15:42.63 ASV.dial :PCRlock @19:15:43.03 delay 5 seconds @19:15:48.05 Running Ss Profile60C350 thermocycling @19:15:48.06 PCR.program Profile60C350 @19:15:48.21 Profile60C350 should complete in < 1:48:05 @19:15:48.22 PCR.run 2:20:06.25 @20:39:12.49 PCR.off @20:39:14.65 PCR for Ss completed -- closed /var/log/esp/Ss19may0619h.pcr @20:39:14.73 Cleaning PCR @20:39:14.74 AFV.select :carrierSolution @20:39:14.86 delay 2 seconds @20:39:16.88 AS.reconfigure ASconfig @20:39:16.94 AS.seek 0.5ml @20:39:27.23 delay 1 second @20:39:28.26 AFV.select :holdingCoil @20:39:28.36 ASV.dial :waste1 @20:39:28.75 ATV.dial :air3 @20:39:30.05 AS.reconfigure ASslow1 @20:39:30.12 AS.seek 0.51ml @20:39:32.36 delay 2 seconds @20:39:34.39 ATV.dial :bleach @20:39:35.03 AS.seek 0.81ml @20:40:35.59 delay 3 seconds @20:40:38.62 ATV.dial :air3 @20:40:39.21 delay 3 seconds @20:40:42.28 AS.seek 0.835ml @20:40:47.64 delay 3 seconds @20:40:50.67 ATV.dial :bleach @20:40:51.30 AS.seek 0.82ml @20:40:54.56 delay 3 seconds @20:40:57.59 ARV.dial :waste @20:40:58.21 delay 3 seconds @20:41:01.23 ATV.dial :ARV @20:41:02.13 AS.reconfigure ASconfig @20:41:02.19 AS.seek 0.47ml @20:41:22.60 AFV.select :carrierSolution @20:41:22.72 delay 2 seconds @20:41:24.74 AS.seek 1ml @20:41:55.52 delay 1 second @20:41:56.55 AFV.select :holdingCoil @20:41:56.65 delay 1 second @20:41:57.67 AS.reconfigure ASfast @20:41:57.73 AS.seek 0ml @20:42:25.07 AFV.select :carrierSolution @20:42:25.19 delay 2 seconds @20:42:27.21 AS.reconfigure ASconfig @20:42:27.27 AS.seek 1ml @20:43:25.06 delay 1 second @20:43:26.09 AFV.select :holdingCoil @20:43:26.19 delay 1 second @20:43:27.21 AS.reconfigure ASfast @20:43:27.27 AS.seek 0ml @20:43:54.60 AFV.select :carrierSolution @20:43:54.72 delay 2 seconds @20:43:56.74 AS.reconfigure ASconfig @20:43:56.80 AS.seek 1ml @20:44:54.54 delay 1 second @20:44:55.57 AFV.select :holdingCoil @20:44:55.67 delay 1 second @20:44:56.69 AS.reconfigure ASfast @20:44:56.75 AS.seek 0ml @20:45:24.09 ARV.dial between carrier and air1 @20:45:24.60 ATV.dial :air3 @20:45:25.90 AS.reconfigure ASslow1 @20:45:25.96 AS.seek 0.01ml @20:45:28.34 delay 2 seconds @20:45:30.37 ATV.dial :bleach @20:45:30.98 AS.seek 0.81ml @20:48:12.18 delay 3 seconds @20:48:15.21 ATV.dial :air3 @20:48:15.77 delay 3 seconds @20:48:18.84 AS.seek 0.835ml @20:48:24.17 delay 3 seconds @20:48:27.19 ATV.dial :bleach @20:48:27.80 AS.seek 0.82ml @20:48:31.09 delay 3 seconds @20:48:34.12 ATV.dial PCR @20:48:34.53 AS.reconfigure ASconfig @20:48:34.59 AS.seek :empty @20:49:22.06 PCR.program :bleachProfile @20:49:22.12 PCR program should complete in < 2:45 @20:49:22.13 PCR.run 10:00 @20:52:17.92 PCR.off @20:52:17.95 AFV.select :carrierSolution @20:52:18.07 delay 2 seconds @20:52:20.09 AS.seek 1ml @20:53:17.91 delay 1 second @20:53:18.94 AFV.select :holdingCoil @20:53:19.04 delay 1 second @20:53:20.06 AS.reconfigure ASfast @20:53:20.12 AS.seek 0ml @20:53:47.49 delay 3 seconds @20:53:50.51 AFV.select :carrierSolution @20:53:50.61 delay 2 seconds @20:53:52.63 AS.reconfigure ASconfig @20:53:52.69 AS.seek 1ml @20:54:50.49 delay 1 second @20:54:51.52 AFV.select :holdingCoil @20:54:51.62 delay 1 second @20:54:52.64 AS.reconfigure ASfast @20:54:52.70 AS.seek 0ml @20:55:20.04 delay 3 seconds @20:55:23.07 AFV.select :carrierSolution @20:55:23.17 delay 2 seconds @20:55:25.19 AS.reconfigure ASconfig @20:55:25.27 AS.seek 1ml @20:56:23.04 delay 1 second @20:56:24.07 AFV.select :holdingCoil @20:56:24.17 delay 1 second @20:56:25.19 AS.reconfigure ASfast @20:56:25.25 AS.seek 0ml @20:56:52.58 delay 3 seconds @20:56:55.60 AFV.select :carrierSolution @20:56:55.70 delay 2 seconds @20:56:57.72 AS.reconfigure ASconfig @20:56:57.78 AS.seek 1ml @20:57:55.57 delay 1 second @20:57:56.60 AFV.select :holdingCoil @20:57:56.70 delay 1 second @20:57:57.72 AS.reconfigure ASfast @20:57:57.78 AS.seek 0ml @20:58:25.20 delay 3 seconds @20:58:28.23 AFV.select :carrierSolution @20:58:28.33 delay 2 seconds @20:58:30.35 AS.reconfigure ASconfig @20:58:30.42 AS.seek 1ml @20:59:28.21 delay 1 second @20:59:29.24 AFV.select :holdingCoil @20:59:29.34 delay 1 second @20:59:30.36 AS.reconfigure ASnorm @20:59:30.42 AS.seek 0ml @21:00:21.14
AFV.select :carrierSolution @21:00:21.25 delay 2 seconds @21:00:23.27 AS.reconfigure ASconfig @21:00:23.33 AS.seek 0.5ml @21:00:52.79 delay 1 second @21:00:53.82 AFV.select :holdingCoil @21:00:53.92 ATV.dial :air1 @21:00:55.42 AS.reconfigure ASslow2 @21:00:55.48 AS.seek 0.51ml @21:01:06.10 delay 5 seconds @21:01:11.12 ATV.dial :extSrc @21:01:12.84 AS.seek 0.53ml @21:01:33.14 delay 10 seconds @21:01:43.17 ATV.dial :air1 @21:01:44.87 AS.reconfigure ASslow1 @21:01:44.93 AS.seek 0.56ml @21:01:51.23 ATV.dial :waste @21:01:51.78 AS.reconfigure ASconfig @21:01:51.84 AS.seek :empty @21:02:24.51 Setting up PCR for Ss -- writing /var/log/esp/Ss19may0621h.pcr @21:02:24.53 Building PCR Slug from extSrc with MM2 mix, PP4 primer @21:02:24.56 Priming with carrier @21:02:24.58 ATV.dial ARV @21:02:26.10 ARV.dial :carrier @21:02:26.44 AS.reconfigure ASnorm @21:02:26.50 AS.seek 0.499ml @21:02:52.07 AFV.select :carrierSolution @21:02:52.19 delay 2 seconds @21:02:54.21 AS.reconfigure ASconfig @21:02:54.28 AS.seek 1ml @21:03:23.35 delay 1 second @21:03:24.37 AFV.select :holdingCoil @21:03:24.47 delay 1 second @21:03:25.49 ATV.dial :waste @21:03:27.18 AS.reconfigure ASnorm @21:03:27.24 AS.seek :empty @21:04:17.99 AFV.select :carrierSolution @21:04:18.12 delay 2 seconds @21:04:20.15 AS.reconfigure ASconfig @21:04:20.22 AS.seek 0.5ml @21:04:49.34 delay 1 second @21:04:50.37 AFV.select :holdingCoil @21:04:50.47 Building Slug @21:04:50.48 ATV.dial :ARV @21:04:52.16 delay 2 seconds @21:04:54.18 ARV.dial :air1 @21:04:54.58 delay 2 seconds @21:04:56.65 AS.reconfigure ASslow2 @21:04:56.72 AS.seek 0.506ml @21:05:03.12 delay 2 seconds @21:05:05.15 ARV.dial :MM2 @21:05:06.64 delay 2 seconds @21:05:08.66 Adding MM2 @21:05:08.75 AS.seek 0.524ml @21:05:27.26 delay 25 seconds @21:05:52.29 ARV.dial :PP4 @21:05:52.69 Adding PP4 @21:05:52.70 delay 2 seconds @21:05:54.78 AS.seek 0.53ml @21:06:01.09 delay 10 seconds @21:06:11.13 ARV.dial :air3 @21:06:11.53 adding ARV air @21:06:11.54 delay 2 seconds @21:06:13.62 AS.seek 0.536ml @21:06:19.84 delay 5 seconds @21:06:24.87 ARV.dial :carrier @21:06:26.13 delay 7 seconds @21:06:33.21 AS.seek 0.594ml @21:07:31.90 delay 5 seconds @21:07:36.93 ATV.dial :extSrc @21:07:37.50 delay 2 seconds @21:07:39.57 AS.seek 0.6ml @21:07:45.82 delay 4 seconds @21:07:49.85 ATV.dial ARV @21:07:50.41 delay 2 seconds @21:07:52.43 ARV.dial :carrier @21:07:52.44 delay 2 seconds @21:07:54.52 AS.seek 0.65ml @21:08:44.93 delay 4 seconds @21:08:48.96 ATV.dial PCR @21:08:49.73 ASV.dial :waste1 @21:08:49.75 ARV.dial :air1 @21:08:50.17 Positioning Slug by 0.324ml @21:08:50.26 AS.seek 0.326ml @21:14:16.35 delay 5 seconds @21:14:21.38 ATV.dial ARV @21:14:22.17 ASV.dial :PCRlock @21:14:22.58 delay 5 seconds @21:14:27.60 Running Ss Profile60C350 thermocycling @21:14:27.61 PCR.program Profile60C350 @21:14:27.76 Profile60C350 should complete in < 1:48:05 @21:14:27.77 PCR.run 2:20:06.25 @22:37:49.25 PCR.off @22:37:51.23 PCR for Ss completed -- closed /var/log/esp/Ss19may0621h.pcr @22:37:51.30 Cleaning PCR @22:37:51.32 AFV.select :carrierSolution @22:37:51.44 delay 2 seconds @22:37:53.46 AS.reconfigure ASconfig @22:37:53.52 AS.seek 0.5ml @22:38:03.85 delay 1 second @22:38:04.88 AFV.select :holdingCoil @22:38:04.98 ASV.dial :waste1 @22:38:05.37 ATV.dial :air3 @22:38:06.68 AS.reconfigure ASslow1 @22:38:06.74 AS.seek 0.51ml @22:38:09.07 delay 2 seconds @22:38:11.10 ATV.dial :bleach @22:38:11.71 AS.seek 0.81ml @22:39:12.29 delay 3 seconds @22:39:15.32 ATV.dial :air3 @22:39:15.88 delay 3 seconds @22:39:18.95 AS.seek 0.835ml @22:39:24.26 delay 3 seconds @22:39:27.28 ATV.dial :bleach @22:39:27.88 AS.seek 0.82ml @22:39:31.31 delay 3 seconds @22:39:34.34 ARV.dial :waste @22:39:34.94 delay 3 seconds @22:39:37.96 ATV.dial :ARV @22:39:38.87 AS.reconfigure ASconfig @22:39:38.93 AS.seek 0.47ml @22:39:59.35 AFV.select :carrierSolution @22:39:59.47 delay 2 seconds @22:40:01.49 AS.seek 1ml @22:40:32.67 delay 1 second @22:40:33.70 AFV.select :holdingCoil @22:40:33.80 delay 1 second @22:40:34.82 AS.reconfigure ASfast @22:40:34.88 AS.seek 0ml @22:41:02.21 AFV.select :carrierSolution @22:41:02.33 delay 2 seconds @22:41:04.35 AS.reconfigure ASconfig @22:41:04.41 AS.seek 1ml @22:42:02.20 delay 1 second @22:42:03.23 AFV.select :holdingCoil @22:42:03.33 delay 1 second @22:42:04.35 AS.reconfigure ASfast @22:42:04.43 AS.seek 0ml @22:42:31.79 AFV.select :carrierSolution @22:42:31.91 delay 2 seconds @22:42:33.93 AS.reconfigure ASconfig @22:42:33.99 AS.seek 1ml @22:43:31.78 delay 1 second @22:43:32.81 AFV.select :holdingCoil @22:43:32.91 delay 1 second @22:43:33.93 AS.reconfigure ASfast @22:43:33.99 AS.seek 0ml @22:44:01.35 ARV.dial between carrier and air1 @22:44:01.86 ATV.dial :air3 @22:44:03.16 AS.reconfigure ASslow1 @22:44:03.23 AS.seek 0.01ml @22:44:05.68 delay 2 seconds @22:44:07.71 ATV.dial :bleach @22:44:08.33 AS.seek 0.81ml @22:46:49.48 delay 3 seconds @22:46:52.51 ATV.dial :air3 @22:46:53.07 delay 3 seconds @22:46:56.14 AS.seek 0.835ml @22:47:01.45 delay 3 seconds @22:47:04.47 ATV.dial :bleach @22:47:05.09 AS.seek 0.82ml @22:47:08.37 delay 3 seconds @22:47:11.40 ATV.dial PCR @22:47:11.82 AS.reconfigure ASconfig @22:47:11.87 AS.seek :empty @22:47:59.34 PCR.program :bleachProfile @22:47:59.40 PCR program should complete in < 2:45 @22:47:59.41 PCR.run 10:00 @22:50:55.19 PCR.off @22:50:55.22 AFV.select :carrierSolution @22:50:55.34 delay 2 seconds @22:50:57.36 AS.seek 1ml @22:51:55.17 delay 1 second @22:51:56.20 AFV.select :holdingCoil @22:51:56.30 delay 1 second @22:51:57.32 AS.reconfigure ASfast @22:51:57.38 AS.seek 0ml @22:52:24.73 delay 3 seconds @22:52:27.76 AFV.select :carrierSolution @22:52:27.86 delay 2 seconds @22:52:29.88 AS.reconfigure ASconfig @22:52:29.95 AS.seek 1ml @22:53:27.75 delay 1 second @22:53:28.78 AFV.select :holdingCoil @22:53:28.88 delay 1 second @22:53:29.90 AS.reconfigure ASfast @22:53:29.96 AS.seek 0ml @22:53:57.33 delay 3 seconds @22:54:00.35 AFV.select :carrierSolution @22:54:00.45 delay 2 seconds @22:54:02.47 AS.reconfigure ASconfig @22:54:02.53 AS.seek 1ml @22:55:00.31 delay 1 second @22:55:01.34 AFV.select :holdingCoil @22:55:01.44 delay 1 second @22:55:02.46 AS.reconfigure ASfast @22:55:02.52 AS.seek 0ml @22:55:29.89 delay 3 seconds @22:55:32.92 AFV.select :carrierSolution @22:55:33.02 delay 2 seconds @22:55:35.04 AS.reconfigure ASconfig @22:55:35.11 AS.seek 1ml @22:56:32.89 delay 1 second @22:56:33.92 AFV.select :holdingCoil @22:56:34.02 delay 1 second @22:56:35.04 AS.reconfigure ASfast @22:56:35.10 AS.seek 0ml @22:57:02.45 delay 3 seconds @22:57:05.48 AFV.select :carrierSolution @22:57:05.58 delay 2 seconds @22:57:07.60 AS.reconfigure ASconfig @22:57:07.67 AS.seek 1ml @22:58:05.45 delay 1 second @22:58:06.48 AFV.select :holdingCoil @22:58:06.58 delay 1 second @22:58:07.60 AS.reconfigure ASnorm @22:58:07.66 AS.seek 0ml @22:58:58.37
AFV.select :carrierSolution @22:58:58.48 delay 2 seconds @22:59:00.50 AS.reconfigure ASconfig @22:59:00.56 AS.seek 0.5ml @22:59:29.60 delay 1 second @22:59:30.63 AFV.select :holdingCoil @22:59:30.73 ATV.dial :air1 @22:59:32.23 AS.reconfigure ASslow2 @22:59:32.30 AS.seek 0.506ml @22:59:38.54 ATV.dial :extSrc @22:59:40.26 AS.seek 0.512ml @22:59:46.45 delay 5 seconds @22:59:51.48 ATV.dial :air1 @22:59:53.59 AS.reconfigure ASslow1 @22:59:53.65 AS.seek 0.542ml @22:59:59.96 ATV.dial :waste @23:00:00.52 AS.reconfigure ASconfig @23:00:00.58 AS.seek :empty @23:00:32.23 Setting up PCR for Ss -- writing /var/log/esp/Ss19may0623h.pcr @23:00:32.25 Building PCR Slug from extSrc with MM2 mix, PP4 primer @23:00:32.28 Priming with carrier @23:00:32.30 ATV.dial ARV @23:00:33.93 ARV.dial :carrier @23:00:34.28 AS.reconfigure ASnorm @23:00:34.34 AS.seek 0.499ml @23:00:59.92 AFV.select :carrierSolution @23:01:00.04 delay 2 seconds @23:01:02.06 AS.reconfigure ASconfig @23:01:02.13 AS.seek 1ml @23:01:31.21 delay 1 second @23:01:32.23 AFV.select :holdingCoil @23:01:32.33 delay 1 second @23:01:33.35 ATV.dial :waste @23:01:35.02 AS.reconfigure ASnorm @23:01:35.08 AS.seek :empty @23:02:25.81 AFV.select :carrierSolution @23:02:25.94 delay 2 seconds @23:02:27.96 AS.reconfigure ASconfig @23:02:28.03 AS.seek 0.5ml @23:02:57.15 delay 1 second @23:02:58.17 AFV.select :holdingCoil @23:02:58.27 Building Slug @23:02:58.28 ATV.dial :ARV @23:02:59.97 delay 2 seconds @23:03:01.99 ARV.dial :air1 @23:03:02.41 delay 2 seconds @23:03:04.48 AS.reconfigure ASslow2 @23:03:04.54 AS.seek 0.506ml @23:03:10.75 delay 2 seconds @23:03:12.78 ARV.dial :MM2 @23:03:14.27 delay 2 seconds @23:03:16.29 Adding MM2 @23:03:16.38 AS.seek 0.524ml @23:03:34.70 delay 25 seconds @23:03:59.73 ARV.dial :PP4 @23:04:00.11 Adding PP4 @23:04:00.12 delay 2 seconds @23:04:02.20 AS.seek 0.53ml @23:04:08.40 delay 10 seconds @23:04:18.44 ARV.dial :air3 @23:04:18.83 adding ARV air @23:04:18.84 delay 2 seconds @23:04:20.92 AS.seek 0.536ml @23:04:27.10 delay 5 seconds @23:04:32.13 ARV.dial :carrier @23:04:33.40 delay 7 seconds @23:04:40.48 AS.seek 0.594ml @23:05:39.03 delay 5 seconds @23:05:44.06 ATV.dial :extSrc @23:05:44.63 delay 2 seconds @23:05:46.70 AS.seek 0.6ml @23:05:52.92 delay 4 seconds @23:05:56.95 ATV.dial ARV @23:05:57.52 delay 2 seconds @23:05:59.54 ARV.dial :carrier @23:05:59.55 delay 2 seconds @23:06:01.63 AS.seek 0.65ml @23:06:52.06 delay 4 seconds @23:06:56.09 ATV.dial PCR @23:06:56.87 ASV.dial :waste1 @23:06:56.89 ARV.dial :air1 @23:06:57.31 Positioning Slug by 0.324ml @23:06:57.40 AS.seek 0.326ml @23:12:23.45 delay 5 seconds @23:12:28.48 ATV.dial ARV @23:12:29.25 ASV.dial :PCRlock @23:12:29.66 delay 5 seconds @23:12:34.68 Running Ss Profile60C350 thermocycling @23:12:34.69 PCR.program Profile60C350 @23:12:34.85 Profile60C350 should complete in < 1:48:05 @23:12:34.86 PCR.run 2:20:06.25 @00:35:57.18CEST07-May-19 PCR.off @00:35:59.16 PCR for Ss completed -- closed /var/log/esp/Ss19may0623h.pcr @00:35:59.24 Cleaning PCR @00:35:59.25 AFV.select :carrierSolution @00:35:59.37 delay 2 seconds @00:36:01.39 AS.reconfigure ASconfig @00:36:01.45 AS.seek 0.5ml @00:36:11.76 delay 1 second @00:36:12.79 AFV.select :holdingCoil @00:36:12.89 ASV.dial :waste1 @00:36:13.28 ATV.dial :air3 @00:36:14.59 AS.reconfigure ASslow1 @00:36:14.65 AS.seek 0.51ml @00:36:16.93 delay 2 seconds @00:36:18.96 ATV.dial :bleach @00:36:19.57 AS.seek 0.81ml @00:37:20.25 delay 3 seconds @00:37:23.28 ATV.dial :air3 @00:37:23.84 delay 3 seconds @00:37:26.91 AS.seek 0.835ml @00:37:32.24 delay 3 seconds @00:37:35.27 ATV.dial :bleach @00:37:35.89 AS.seek 0.82ml @00:37:39.22 delay 3 seconds @00:37:42.25 ARV.dial :waste @00:37:42.87 delay 3 seconds @00:37:45.89 ATV.dial :ARV @00:37:46.79 AS.reconfigure ASconfig @00:37:46.85 AS.seek 0.47ml @00:38:07.25 AFV.select :carrierSolution @00:38:07.37 delay 2 seconds @00:38:09.39 AS.seek 1ml @00:38:40.18 delay 1 second @00:38:41.21 AFV.select :holdingCoil @00:38:41.31 delay 1 second @00:38:42.33 AS.reconfigure ASfast @00:38:42.39 AS.seek 0ml @00:39:09.84 AFV.select :carrierSolution @00:39:09.96 delay 2 seconds @00:39:11.98 AS.reconfigure ASconfig @00:39:12.42 AS.seek 1ml @00:40:10.15 delay 1 second @00:40:11.18 AFV.select :holdingCoil @00:40:11.28 delay 1 second @00:40:12.30 AS.reconfigure ASfast @00:40:12.36 AS.seek 0ml @00:40:39.70 AFV.select :carrierSolution @00:40:39.83 delay 2 seconds @00:40:41.85 AS.reconfigure ASconfig @00:40:41.91 AS.seek 1ml @00:41:39.67 delay 1 second @00:41:40.70 AFV.select :holdingCoil @00:41:40.80 delay 1 second @00:41:41.82 AS.reconfigure ASfast @00:41:41.89 AS.seek 0ml @00:42:09.25 ARV.dial between carrier and air1 @00:42:09.76 ATV.dial :air3 @00:42:11.07 AS.reconfigure ASslow1 @00:42:11.14 AS.seek 0.01ml @00:42:13.50 delay 2 seconds @00:42:15.53 ATV.dial :bleach @00:42:16.16 AS.seek 0.81ml @00:44:57.29 delay 3 seconds @00:45:00.32 ATV.dial :air3 @00:45:00.88 delay 3 seconds @00:45:03.95 AS.seek 0.835ml @00:45:09.30 delay 3 seconds @00:45:12.32 ATV.dial :bleach @00:45:12.94 AS.seek 0.82ml @00:45:16.19 delay 3 seconds @00:45:19.22 ATV.dial PCR @00:45:19.63 AS.reconfigure ASconfig @00:45:19.68 AS.seek :empty @00:46:07.15 PCR.program :bleachProfile @00:46:07.21 PCR program should complete in < 2:45 @00:46:07.22 PCR.run 10:00 @00:49:03.00 PCR.off @00:49:03.03 AFV.select :carrierSolution @00:49:03.14 delay 2 seconds @00:49:05.16 AS.seek 1ml @00:50:02.98 delay 1 second @00:50:04.01 AFV.select :holdingCoil @00:50:04.11 delay 1 second @00:50:05.13 AS.reconfigure ASfast @00:50:05.19 AS.seek 0ml @00:50:32.54 delay 3 seconds @00:50:35.57 AFV.select :carrierSolution @00:50:35.67 delay 2 seconds @00:50:37.69 AS.reconfigure ASconfig @00:50:37.75 AS.seek 1ml @00:51:35.54 delay 1 second @00:51:36.57 AFV.select :holdingCoil @00:51:36.67 delay 1 second @00:51:37.69 AS.reconfigure ASfast @00:51:37.75 AS.seek 0ml @00:52:05.12 delay 3 seconds @00:52:08.14 AFV.select :carrierSolution @00:52:08.24 delay 2 seconds @00:52:10.26 AS.reconfigure ASconfig @00:52:10.33 AS.seek 1ml @00:53:08.08 delay 1 second @00:53:09.11 AFV.select :holdingCoil @00:53:09.21 delay 1 second @00:53:10.23 AS.reconfigure ASfast @00:53:10.29 AS.seek 0ml @00:53:37.73 delay 3 seconds @00:53:40.76 AFV.select :carrierSolution @00:53:40.86 delay 2 seconds @00:53:42.88 AS.reconfigure ASconfig @00:53:42.94 AS.seek 1ml @00:54:40.73 delay 1 second @00:54:41.76 AFV.select :holdingCoil @00:54:41.86 delay 1 second @00:54:42.88 AS.reconfigure ASfast @00:54:42.94 AS.seek 0ml @00:55:10.29 delay 3 seconds @00:55:13.32 AFV.select :carrierSolution @00:55:13.42 delay 2 seconds @00:55:15.44 AS.reconfigure ASconfig @00:55:15.51 AS.seek 1ml @00:56:13.29 delay 1 second @00:56:14.32 AFV.select :holdingCoil @00:56:14.42 delay 1 second @00:56:15.44 AS.reconfigure ASnorm @00:56:15.50 AS.seek 0ml @00:57:06.21
AFV.select :carrierSolution @00:57:06.32 delay 2 seconds @00:57:08.34 AS.reconfigure ASconfig @00:57:08.40 AS.seek 0.5ml @00:57:37.44 delay 1 second @00:57:38.47 AFV.select :holdingCoil @00:57:38.57 ATV.dial :air1 @00:57:40.08 AS.reconfigure ASslow1 @00:57:40.13 AS.seek 0.52ml @00:57:44.42 delay 5 seconds @00:57:49.45 ATV.dial :extSrc @00:57:51.17 AS.reconfigure ASslow2 @00:57:51.23 AS.seek 0.545ml @00:58:16.61 delay 10 seconds @00:58:26.64 ATV.dial :air1 @00:58:28.35 AS.reconfigure ASslow1 @00:58:28.41 AS.seek 0.575ml @00:58:34.76 ATV.dial :waste @00:58:35.66 AS.reconfigure ASconfig @00:58:35.77 AS.seek :empty @00:59:09.33 Setting up PCR for Ss -- writing /var/log/esp/Ss19may0700h.pcr @00:59:09.35 Building PCR Slug from extSrc with MM2 mix, PP4 primer @00:59:09.38 Priming with carrier @00:59:09.39 ATV.dial ARV @00:59:10.90 ARV.dial :carrier @00:59:11.26 AS.reconfigure ASnorm @00:59:11.32 AS.seek 0.499ml @00:59:36.89 AFV.select :carrierSolution @00:59:37.01 delay 2 seconds @00:59:39.03 AS.reconfigure ASconfig @00:59:39.10 AS.seek 1ml @01:00:08.20 delay 1 second @01:00:09.22 AFV.select :holdingCoil @01:00:09.32 delay 1 second @01:00:10.34 ATV.dial :waste @01:00:12.02 AS.reconfigure ASnorm @01:00:12.08 AS.seek :empty @01:01:02.87 AFV.select :carrierSolution @01:01:03.00 delay 2 seconds @01:01:05.02 AS.reconfigure ASconfig @01:01:05.09 AS.seek 0.5ml @01:01:34.18 delay 1 second @01:01:35.20 AFV.select :holdingCoil @01:01:35.30 Building Slug @01:01:35.31 ATV.dial :ARV @01:01:37.09 delay 2 seconds @01:01:39.11 ARV.dial :air1 @01:01:39.53 delay 2 seconds @01:01:41.60 AS.reconfigure ASslow2 @01:01:41.66 AS.seek 0.506ml @01:01:47.95 delay 2 seconds @01:01:49.98 ARV.dial :MM2 @01:01:51.46 delay 2 seconds @01:01:53.48 Adding MM2 @01:01:53.57 AS.seek 0.524ml @01:02:12.08 delay 25 seconds @01:02:37.11 ARV.dial :PP4 @01:02:37.51 Adding PP4 @01:02:37.52 delay 2 seconds @01:02:39.60 AS.seek 0.53ml @01:02:46.01 delay 10 seconds @01:02:56.05 ARV.dial :air3 @01:02:56.46 adding ARV air @01:02:56.47 delay 2 seconds @01:02:58.55 AS.seek 0.536ml @01:03:04.75 delay 5 seconds @01:03:09.78 ARV.dial :carrier @01:03:11.04 delay 7 seconds @01:03:18.12 AS.seek 0.594ml @01:04:16.86 delay 5 seconds @01:04:21.89 ATV.dial :extSrc @01:04:22.46 delay 2 seconds @01:04:24.53 AS.seek 0.6ml @01:04:30.89 delay 4 seconds @01:04:34.92 ATV.dial ARV @01:04:35.49 delay 2 seconds @01:04:37.51 ARV.dial :carrier @01:04:37.52 delay 2 seconds @01:04:39.60 AS.seek 0.65ml @01:05:30.28 delay 4 seconds @01:05:34.31 ATV.dial PCR @01:05:35.09 ASV.dial :waste1 @01:05:35.11 ARV.dial :air1 @01:05:35.54 Positioning Slug by 0.324ml @01:05:35.63 AS.seek 0.326ml @01:11:01.86 delay 5 seconds @01:11:06.89 ATV.dial ARV @01:11:07.67 ASV.dial :PCRlock @01:11:08.09 delay 5 seconds @01:11:13.11 Running Ss Profile60C350 thermocycling @01:11:13.12 PCR.program Profile60C350 @01:11:13.27 Profile60C350 should complete in < 1:48:05 @01:11:13.28 PCR.run 2:20:06.25 @02:34:30.21 PCR.off @02:34:32.19 PCR for Ss completed -- closed /var/log/esp/Ss19may0700h.pcr @02:34:32.26 Cleaning PCR @02:34:32.27 AFV.select :carrierSolution @02:34:32.40 delay 2 seconds @02:34:34.42 AS.reconfigure ASconfig @02:34:34.48 AS.seek 0.5ml @02:34:44.81 delay 1 second @02:34:45.84 AFV.select :holdingCoil @02:34:45.94 ASV.dial :waste1 @02:34:46.34 ATV.dial :air3 @02:34:47.64 AS.reconfigure ASslow1 @02:34:47.71 AS.seek 0.51ml @02:34:50.03 delay 2 seconds @02:34:52.06 ATV.dial :bleach @02:34:52.67 AS.seek 0.81ml @02:35:53.39 delay 3 seconds @02:35:56.42 ATV.dial :air3 @02:35:56.98 delay 3 seconds @02:36:00.05 AS.seek 0.835ml @02:36:05.36 delay 3 seconds @02:36:08.38 ATV.dial :bleach @02:36:08.98 AS.seek 0.82ml @02:36:12.27 delay 3 seconds @02:36:15.30 ARV.dial :waste @02:36:15.90 delay 3 seconds @02:36:18.92 ATV.dial :ARV @02:36:19.83 AS.reconfigure ASconfig @02:36:19.89 AS.seek 0.47ml @02:36:40.31 AFV.select :carrierSolution @02:36:40.43 delay 2 seconds @02:36:42.45 AS.seek 1ml @02:37:13.23 delay 1 second @02:37:14.26 AFV.select :holdingCoil @02:37:14.36 delay 1 second @02:37:15.38 AS.reconfigure ASfast @02:37:15.44 AS.seek 0ml @02:37:42.78 AFV.select :carrierSolution @02:37:42.90 delay 2 seconds @02:37:44.92 AS.reconfigure ASconfig @02:37:44.98 AS.seek 1ml @02:38:42.73 delay 1 second @02:38:43.76 AFV.select :holdingCoil @02:38:43.86 delay 1 second @02:38:44.88 AS.reconfigure ASfast @02:38:44.94 AS.seek 0ml @02:39:12.28 AFV.select :carrierSolution @02:39:12.77 delay 2 seconds @02:39:14.79 AS.reconfigure ASconfig @02:39:14.85 AS.seek 1ml @02:40:12.61 delay 1 second @02:40:13.64 AFV.select :holdingCoil @02:40:13.74 delay 1 second @02:40:14.76 AS.reconfigure ASfast @02:40:14.82 AS.seek 0ml @02:40:42.17 ARV.dial between carrier and air1 @02:40:42.68 ATV.dial :air3 @02:40:43.98 AS.reconfigure ASslow1 @02:40:44.04 AS.seek 0.01ml @02:40:46.41 delay 2 seconds @02:40:48.44 ATV.dial :bleach @02:40:49.07 AS.seek 0.81ml @02:43:30.22 delay 3 seconds @02:43:33.25 ATV.dial :air3 @02:43:33.81 delay 3 seconds @02:43:36.88 AS.seek 0.835ml @02:43:42.19 delay 3 seconds @02:43:45.21 ATV.dial :bleach @02:43:45.82 AS.seek 0.82ml @02:43:49.09 delay 3 seconds @02:43:52.12 ATV.dial PCR @02:43:52.54 AS.reconfigure ASconfig @02:43:52.59 AS.seek :empty @02:44:40.05 PCR.program :bleachProfile @02:44:40.11 PCR program should complete in < 2:45 @02:44:40.12 PCR.run 10:00 @02:47:35.91 PCR.off @02:47:35.94 AFV.select :carrierSolution @02:47:36.05 delay 2 seconds @02:47:38.07 AS.seek 1ml @02:48:35.88 delay 1 second @02:48:36.91 AFV.select :holdingCoil @02:48:37.01 delay 1 second @02:48:38.03 AS.reconfigure ASfast @02:48:38.09 AS.seek 0ml @02:49:05.44 delay 3 seconds @02:49:08.47 AFV.select :carrierSolution @02:49:08.57 delay 2 seconds @02:49:10.59 AS.reconfigure ASconfig @02:49:10.66 AS.seek 1ml @02:50:08.45 delay 1 second @02:50:09.48 AFV.select :holdingCoil @02:50:09.58 delay 1 second @02:50:10.60 AS.reconfigure ASfast @02:50:10.66 AS.seek 0ml @02:50:38.05 delay 3 seconds @02:50:41.08 AFV.select :carrierSolution @02:50:41.18 delay 2 seconds @02:50:43.20 AS.reconfigure ASconfig @02:50:43.26 AS.seek 1ml @02:51:41.06 delay 1 second @02:51:42.09 AFV.select :holdingCoil @02:51:42.19 delay 1 second @02:51:43.21 AS.reconfigure ASfast @02:51:43.27 AS.seek 0ml @02:52:10.61 delay 3 seconds @02:52:13.64 AFV.select :carrierSolution @02:52:13.74 delay 2 seconds @02:52:15.76 AS.reconfigure ASconfig @02:52:15.82 AS.seek 1ml @02:53:13.59 delay 1 second @02:53:14.62 AFV.select :holdingCoil @02:53:14.72 delay 1 second @02:53:15.74 AS.reconfigure ASfast @02:53:15.80 AS.seek 0ml @02:53:43.16 delay 3 seconds @02:53:46.19 AFV.select :carrierSolution @02:53:46.29 delay 2 seconds @02:53:48.31 AS.reconfigure ASconfig @02:53:48.38 AS.seek 1ml @02:54:46.16 delay 1 second @02:54:47.19 AFV.select :holdingCoil @02:54:47.29 delay 1 second @02:54:48.31 AS.reconfigure ASnorm @02:54:48.37 AS.seek 0ml @02:55:39.08
AFV.select :carrierSolution @02:55:39.19 delay 2 seconds @02:55:41.21 AS.reconfigure ASconfig @02:55:41.27 AS.seek 0.5ml @02:55:55.14 Session Ended @02:56:10.35
delay 1 second @02:56:11.37 AFV.select :holdingCoil @02:56:11.46 ATV.dial :air1 @02:56:12.95 AS.reconfigure ASslow2 @02:56:12.99 AS.seek 0.506ml @02:56:19.22 ATV.dial :extSrc @02:56:20.92 AS.seek 0.512ml @02:56:27.34 delay 5 seconds @02:56:32.36 ATV.dial :air1 @02:56:34.08 AS.reconfigure ASslow1 @02:56:34.12 AS.seek 0.542ml @02:56:40.44 ATV.dial :waste @02:56:41.00 AS.reconfigure ASconfig @02:56:41.04 AS.seek :empty @02:57:12.70 Setting up PCR for Ss -- writing /var/log/esp/Ss19may0702h.pcr @02:57:12.72 Building PCR Slug from extSrc with MM2 mix, PP4 primer @02:57:12.73 Priming with carrier @02:57:12.74 ATV.dial ARV @02:57:14.61 ARV.dial :carrier @02:57:14.96 AS.reconfigure ASnorm @02:57:15.00 AS.seek 0.499ml @02:57:40.56 AFV.select :carrierSolution @02:57:40.67 delay 2 seconds @02:57:42.69 AS.reconfigure ASconfig @02:57:42.74 AS.seek 1ml @02:58:11.82 delay 1 second @02:58:12.84 AFV.select :holdingCoil @02:58:12.94 delay 1 second @02:58:13.96 ATV.dial :waste @02:58:15.64 AS.reconfigure ASnorm @02:58:15.68 AS.seek :empty @02:59:06.39 AFV.select :carrierSolution @02:59:06.50 delay 2 seconds @02:59:08.52 AS.reconfigure ASconfig @02:59:08.57 AS.seek 0.5ml @02:59:37.67 delay 1 second @02:59:38.70 AFV.select :holdingCoil @02:59:38.80 Building Slug @02:59:38.81 ATV.dial :ARV @02:59:40.46 delay 2 seconds @02:59:42.48 ARV.dial :air1 @02:59:42.87 delay 2 seconds @02:59:44.94 AS.reconfigure ASslow2 @02:59:44.98 AS.seek 0.506ml @02:59:51.16 delay 2 seconds @02:59:53.19 ARV.dial :MM2 @02:59:54.65 delay 2 seconds @02:59:56.67 Adding MM2 @02:59:56.73 AS.seek 0.524ml @03:00:14.91 delay 25 seconds @03:00:39.94 ARV.dial :PP4 @03:00:40.34 Adding PP4 @03:00:40.35 delay 2 seconds @03:00:42.42 AS.seek 0.53ml @03:00:48.81 delay 10 seconds @03:00:58.84 ARV.dial :air3 @03:00:59.21 adding ARV air @03:00:59.22 delay 2 seconds @03:01:01.29 AS.seek 0.536ml @03:01:07.61 delay 5 seconds @03:01:12.64 ARV.dial :carrier @03:01:13.90 delay 7 seconds @03:01:20.98 AS.seek 0.594ml @03:02:19.66 delay 5 seconds @03:02:24.69 ATV.dial :extSrc @03:02:25.25 delay 2 seconds @03:02:27.32 AS.seek 0.6ml @03:02:33.58 delay 4 seconds @03:02:37.61 ATV.dial ARV @03:02:38.15 delay 2 seconds @03:02:40.17 ARV.dial :carrier @03:02:40.18 delay 2 seconds @03:02:42.25 AS.seek 0.65ml @03:03:32.67 delay 4 seconds @03:03:36.70 ATV.dial PCR @03:03:37.45 ASV.dial :waste1 @03:03:37.47 ARV.dial :air1 @03:03:37.84 Positioning Slug by 0.324ml @03:03:37.91 AS.seek 0.326ml @03:09:04.05 delay 5 seconds @03:09:09.08 ATV.dial ARV @03:09:09.85 ASV.dial :PCRlock @03:09:10.25 delay 5 seconds @03:09:15.27 Running Ss Profile60C350 thermocycling @03:09:15.28 PCR.program Profile60C350 @03:09:15.41 Profile60C350 should complete in < 1:48:05 @03:09:15.42 PCR.run 2:20:06.25 @04:32:30.27 PCR.off @04:32:32.24 PCR for Ss completed -- closed /var/log/esp/Ss19may0702h.pcr @04:32:32.30 Cleaning PCR @04:32:32.31 AFV.select :carrierSolution @04:32:32.41 delay 2 seconds @04:32:34.43 AS.reconfigure ASconfig @04:32:34.48 AS.seek 0.5ml @04:32:44.75 delay 1 second @04:32:45.78 AFV.select :holdingCoil @04:32:45.88 ASV.dial :waste1 @04:32:46.28 ATV.dial :air3 @04:32:47.56 AS.reconfigure ASslow1 @04:32:47.61 AS.seek 0.51ml @04:32:49.89 delay 2 seconds @04:32:51.92 ATV.dial :bleach @04:32:52.52 AS.seek 0.81ml @04:33:53.10 delay 3 seconds @04:33:56.13 ATV.dial :air3 @04:33:56.67 delay 3 seconds @04:33:59.74 AS.seek 0.835ml @04:34:05.02 delay 3 seconds @04:34:08.05 ATV.dial :bleach @04:34:08.64 AS.seek 0.82ml @04:34:12.03 delay 3 seconds @04:34:15.06 ARV.dial :waste @04:34:15.65 delay 3 seconds @04:34:18.67 ATV.dial :ARV @04:34:19.66 AS.reconfigure ASconfig @04:34:19.70 AS.seek 0.47ml @04:34:40.10 AFV.select :carrierSolution @04:34:40.21 delay 2 seconds @04:34:42.23 AS.seek 1ml @04:35:12.97 delay 1 second @04:35:14.00 AFV.select :holdingCoil @04:35:14.10 delay 1 second @04:35:15.12 AS.reconfigure ASfast @04:35:15.17 AS.seek 0ml @04:35:42.49 AFV.select :carrierSolution @04:35:42.60 delay 2 seconds @04:35:44.62 AS.reconfigure ASconfig @04:35:44.67 AS.seek 1ml @04:36:42.39 delay 1 second @04:36:43.42 AFV.select :holdingCoil @04:36:43.52 delay 1 second @04:36:44.54 AS.reconfigure ASfast @04:36:44.59 AS.seek 0ml @04:37:12.00 AFV.select :carrierSolution @04:37:12.11 delay 2 seconds @04:37:14.13 AS.reconfigure ASconfig @04:37:14.18 AS.seek 1ml @04:38:11.91 delay 1 second @04:38:12.94 AFV.select :holdingCoil @04:38:13.04 delay 1 second @04:38:14.06 AS.reconfigure ASfast @04:38:14.11 AS.seek 0ml @04:38:41.44 ARV.dial between carrier and air1 @04:38:42.31 ATV.dial :air3 @04:38:43.60 AS.reconfigure ASslow1 @04:38:43.65 AS.seek 0.01ml @04:38:45.97 delay 2 seconds @04:38:48.00 ATV.dial :bleach @04:38:48.62 AS.seek 0.81ml @04:41:29.75 delay 3 seconds @04:41:32.78 ATV.dial :air3 @04:41:33.34 delay 3 seconds @04:41:36.41 AS.seek 0.835ml @04:41:41.71 delay 3 seconds @04:41:44.73 ATV.dial :bleach @04:41:45.36 AS.seek 0.82ml @04:41:48.60 delay 3 seconds @04:41:51.63 ATV.dial PCR @04:41:52.03 AS.reconfigure ASconfig @04:41:52.07 AS.seek :empty @04:42:39.50 PCR.program :bleachProfile @04:42:39.56 PCR program should complete in < 2:45 @04:42:39.57 PCR.run 10:00 @04:45:35.34 PCR.off @04:45:35.36 AFV.select :carrierSolution @04:45:35.46 delay 2 seconds @04:45:37.48 AS.seek 1ml @04:46:35.29 delay 1 second @04:46:36.32 AFV.select :holdingCoil @04:46:36.42 delay 1 second @04:46:37.44 AS.reconfigure ASfast @04:46:37.49 AS.seek 0ml @04:47:04.83 delay 3 seconds @04:47:07.86 AFV.select :carrierSolution @04:47:07.96 delay 2 seconds @04:47:09.98 AS.reconfigure ASconfig @04:47:10.04 AS.seek 1ml @04:48:07.79 delay 1 second @04:48:08.82 AFV.select :holdingCoil @04:48:08.92 delay 1 second @04:48:09.94 AS.reconfigure ASfast @04:48:09.99 AS.seek 0ml @04:48:37.33 delay 3 seconds @04:48:40.36 AFV.select :carrierSolution @04:48:40.46 delay 2 seconds @04:48:42.48 AS.reconfigure ASconfig @04:48:42.53 AS.seek 1ml @04:49:40.30 delay 1 second @04:49:41.33 AFV.select :holdingCoil @04:49:41.43 delay 1 second @04:49:42.45 AS.reconfigure ASfast @04:49:42.50 AS.seek 0ml @04:50:09.84 delay 3 seconds @04:50:12.86 AFV.select :carrierSolution @04:50:12.96 delay 2 seconds @04:50:14.98 AS.reconfigure ASconfig @04:50:15.04 AS.seek 1ml @04:51:12.80 delay 1 second @04:51:13.83 AFV.select :holdingCoil @04:51:13.93 delay 1 second @04:51:14.95 AS.reconfigure ASfast @04:51:15.00 AS.seek 0ml @04:51:42.39 delay 3 seconds @04:51:45.42 AFV.select :carrierSolution @04:51:45.52 delay 2 seconds @04:51:47.54 AS.reconfigure ASconfig @04:51:47.59 AS.seek 1ml @04:52:45.36 delay 1 second @04:52:46.39 AFV.select :holdingCoil @04:52:46.49 delay 1 second @04:52:47.51 AS.reconfigure ASnorm @04:52:47.56 AS.seek 0ml @04:53:38.27
AFV.select :carrierSolution @04:53:38.37 delay 2 seconds @04:53:40.39 AS.reconfigure ASconfig @04:53:40.44 AS.seek 0.5ml @04:54:09.49 delay 1 second @04:54:10.52 AFV.select :holdingCoil @04:54:10.62 ATV.dial :air1 @04:54:12.13 AS.reconfigure ASslow1 @04:54:12.17 AS.seek 0.52ml @04:54:16.44 delay 5 seconds @04:54:21.47 ATV.dial :extSrc @04:54:23.17 AS.reconfigure ASslow2 @04:54:23.21 AS.seek 0.538ml @04:54:41.48 delay 10 seconds @04:54:51.50 ATV.dial :air1 @04:54:53.20 AS.reconfigure ASslow1 @04:54:53.24 AS.seek 0.568ml @04:54:59.60 ATV.dial :waste @04:55:00.14 AS.reconfigure ASconfig @04:55:00.18 AS.seek :empty @04:55:33.18 deconEluateRecoveryLine extSrc @04:55:33.19 ATV.dial :waste @04:55:33.21 AS.reconfigure ASnorm @04:55:33.25 AS.seek 0ml @04:55:33.55 ATV.dial :air3 @04:55:34.24 AS.reconfigure ASslow1 @04:55:34.28 AS.seek 0.01ml @04:55:36.58 delay 2 seconds @04:55:38.61 ATV.dial :bleach @04:55:39.06 AS.seek 0.31ml @04:56:39.66 delay 3 seconds @04:56:42.69 ATV.dial :air3 @04:56:43.53 delay 3 seconds @04:56:46.68 AS.seek 0.335ml @04:56:51.97 delay 3 seconds @04:56:55.00 ATV.dial :bleach @04:56:55.61 AS.seek 0.32ml @04:56:58.88 delay 3 seconds @04:57:01.91 ATV.dial :extSrc @04:57:02.62 AS.seek 0ml @04:58:07.22 AFV.select :carrierSolution @04:58:07.33 delay 2 seconds @04:58:09.35 AS.reconfigure ASconfig @04:58:09.40 AS.seek 0.5ml @04:58:38.43 delay 1 second @04:58:39.46 AFV.select :holdingCoil @04:58:39.56 ATV.dial :extSrc @04:58:39.58 AS.reconfigure ASslow1 @04:58:39.62 AS.seek 0ml @05:00:20.44 AFV.select :carrierSolution @05:00:20.55 delay 2 seconds @05:00:22.57 AS.reconfigure ASconfig @05:00:22.62 AS.seek 0.5ml @05:00:51.65 delay 1 second @05:00:52.67 AFV.select :holdingCoil @05:00:52.77 ATV.dial :extSrc @05:00:52.78 AS.reconfigure ASslow1 @05:00:52.83 AS.seek 0ml @05:02:33.63 AFV.select :carrierSolution @05:02:33.74 delay 2 seconds @05:02:35.76 AS.reconfigure ASconfig @05:02:35.81 AS.seek 0.5ml @05:03:04.85 delay 1 second @05:03:05.88 AFV.select :holdingCoil @05:03:05.98 ATV.dial :extSrc @05:03:05.99 AS.reconfigure ASslow1 @05:03:06.04 AS.seek 0ml @05:04:46.86 AFV.select :carrierSolution @05:04:46.97 delay 2 seconds @05:04:48.99 AS.reconfigure ASconfig @05:04:49.04 AS.seek 0.5ml @05:05:18.07 delay 1 second @05:05:19.10 AFV.select :holdingCoil @05:05:19.20 ATV.dial :extSrc @05:05:19.21 AS.reconfigure ASslow1 @05:05:19.26 AS.seek 0ml @05:07:00.07 ATV.dial :air2 @05:07:01.41 AS.reconfigure ASslow0 @05:07:01.46 AS.seek 0.5ml @05:07:52.08 ATV.dial :extSrc @05:07:53.56 AS.seek 0.02ml @05:08:42.16 delay 1 second @05:08:43.19 ATV.dial :waste @05:08:44.48 AS.reconfigure ASconfig @05:08:44.52 AS.seek :empty @05:08:45.99 deconEluateRecoveryLine extSrc @05:08:46.00 ATV.dial :waste @05:08:46.02 AS.reconfigure ASnorm @05:08:46.06 AS.seek 0ml @05:08:46.35 ATV.dial :air3 @05:08:47.21 AS.reconfigure ASslow1 @05:08:47.25 AS.seek 0.01ml @05:08:49.50 delay 2 seconds @05:08:51.53 ATV.dial :bleach @05:08:51.97 AS.seek 0.31ml @05:09:52.55 delay 3 seconds @05:09:55.58 ATV.dial :air3 @05:09:56.12 delay 3 seconds @05:09:59.19 AS.seek 0.335ml @05:10:04.47 delay 3 seconds @05:10:07.50 ATV.dial :bleach @05:10:08.10 AS.seek 0.32ml @05:10:11.41 delay 3 seconds @05:10:14.44 ATV.dial :extSrc @05:10:15.06 AS.seek 0ml @05:11:19.65 AFV.select :carrierSolution @05:11:19.76 delay 2 seconds @05:11:21.78 AS.reconfigure ASconfig @05:11:21.83 AS.seek 0.5ml @05:11:50.83 delay 1 second @05:11:51.86 AFV.select :holdingCoil @05:11:51.96 ATV.dial :extSrc @05:11:51.97 AS.reconfigure ASslow1 @05:11:52.02 AS.seek 0ml @05:13:32.83 AFV.select :carrierSolution @05:13:32.94 delay 2 seconds @05:13:34.96 AS.reconfigure ASconfig @05:13:35.01 AS.seek 0.5ml @05:14:04.04 delay 1 second @05:14:05.07 AFV.select :holdingCoil @05:14:05.17 ATV.dial :extSrc @05:14:05.18 AS.reconfigure ASslow1 @05:14:05.23 AS.seek 0ml @05:15:46.04 AFV.select :carrierSolution @05:15:46.15 delay 2 seconds @05:15:48.17 AS.reconfigure ASconfig @05:15:48.22 AS.seek 0.5ml @05:16:17.24 delay 1 second @05:16:18.27 AFV.select :holdingCoil @05:16:18.37 ATV.dial :extSrc @05:16:18.38 AS.reconfigure ASslow1 @05:16:18.43 AS.seek 0ml @05:17:59.25 AFV.select :carrierSolution @05:17:59.36 delay 2 seconds @05:18:01.38 AS.reconfigure ASconfig @05:18:01.43 AS.seek 0.5ml @05:18:30.46 delay 1 second @05:18:31.49 AFV.select :holdingCoil @05:18:31.59 ATV.dial :extSrc @05:18:31.60 AS.reconfigure ASslow1 @05:18:31.65 AS.seek 0ml @05:20:12.47 ATV.dial :air2 @05:20:13.82 AS.reconfigure ASslow0 @05:20:13.87 AS.seek 0.5ml @05:21:04.47 ATV.dial :extSrc @05:21:05.96 AS.seek 0.02ml @05:21:54.53 delay 1 second @05:21:55.90 ATV.dial :waste @05:21:57.20 AS.reconfigure ASconfig @05:21:57.24 AS.seek :empty @05:21:58.72 deconEluateRecoveryLine extSrc ATV.dial :waste @05:21:58.74 AS.reconfigure ASnorm @05:21:58.78 AS.seek 0ml @05:21:59.10 ATV.dial :air3 @05:21:59.95 AS.reconfigure ASslow1 @05:21:59.99 AS.seek 0.01ml @05:22:02.25 delay 2 seconds @05:22:04.28 ATV.dial :bleach @05:22:04.73 AS.seek 0.31ml @05:23:05.30 delay 3 seconds @05:23:08.33 ATV.dial :air3 @05:23:08.87 delay 3 seconds @05:23:11.94 AS.seek 0.335ml @05:23:17.23 delay 3 seconds @05:23:20.26 ATV.dial :bleach @05:23:20.87 AS.seek 0.32ml @05:23:24.17 delay 3 seconds @05:23:27.19 ATV.dial :extSrc @05:23:27.81 AS.seek 0ml @05:24:32.41 AFV.select :carrierSolution @05:24:32.52 delay 2 seconds @05:24:34.54 AS.reconfigure ASconfig @05:24:34.59 AS.seek 0.5ml @05:25:03.61 delay 1 second @05:25:04.64 AFV.select :holdingCoil @05:25:04.74 ATV.dial :extSrc @05:25:04.75 AS.reconfigure ASslow1 @05:25:04.80 AS.seek 0ml @05:26:45.61 AFV.select :carrierSolution @05:26:45.72 delay 2 seconds @05:26:47.74 AS.reconfigure ASconfig @05:26:47.79 AS.seek 0.5ml @05:27:16.82 delay 1 second @05:27:17.85 AFV.select :holdingCoil @05:27:17.95 ATV.dial :extSrc @05:27:17.96 AS.reconfigure ASslow1 @05:27:18.01 AS.seek 0ml @05:28:58.82 AFV.select :carrierSolution @05:28:58.93 delay 2 seconds @05:29:00.95 AS.reconfigure ASconfig @05:29:01.00 AS.seek 0.5ml @05:29:30.01 delay 1 second @05:29:31.04 AFV.select :holdingCoil @05:29:31.14 ATV.dial :extSrc @05:29:31.15 AS.reconfigure ASslow1 @05:29:31.20 AS.seek 0ml @05:31:12.02 AFV.select :carrierSolution @05:31:12.13 delay 2 seconds @05:31:14.15 AS.reconfigure ASconfig @05:31:14.20 AS.seek 0.5ml @05:31:43.21 delay 1 second @05:31:44.24 AFV.select :holdingCoil @05:31:44.34 ATV.dial :extSrc @05:31:44.35 AS.reconfigure ASslow1 @05:31:44.40 AS.seek 0ml @05:33:25.21 ATV.dial :air2 @05:33:26.55 AS.reconfigure ASslow0 @05:33:26.59 AS.seek 0.5ml @05:34:17.21 ATV.dial :extSrc @05:34:18.70 AS.seek 0.02ml @05:35:07.27 delay 1 second @05:35:08.30 ATV.dial :waste @05:35:09.59 AS.reconfigure ASconfig @05:35:09.63 AS.seek :empty @17:01:06.07CEST07-May-19
ESP2 v3.30 I2C Gateway v3.06 for core bus @17:01:07.78 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @17:01:08.05 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @17:01:12.39 Polling Can every 10:00 @17:01:14.14 SPE.coast @17:01:14.19 SPE.configure SPEconfig @17:01:14.27 AS.reconfigure ASconfig @17:01:14.31 AS.hold @17:01:14.35 AS.configure ASconfig @17:01:14.44 SS.configure SSconfig @17:01:14.52 CC.configure CCconfig @17:01:14.60 CS.configure CSconfig @17:01:14.68 PC.configure PCconfig @17:01:14.76 PS.configure PSconfig @17:01:14.86 Forearm.configure LooseForearm @17:01:14.94 Elbow.configure LooseElbow @17:01:15.39 SC.configure SCloose @17:01:15.47 SE.configure SEconfig @17:01:15.51 CH.coast @17:01:15.55 CH.configure CHconfig @17:01:15.58 PH.coast @17:01:15.63 PH.configure PHconfig @17:01:15.67 PCR.off @17:01:15.69 PCR.pidtemp= 50,0.1,0,300,25,25 @17:01:15.72 PCR.caltemp= 2.8814,0.0372,0 @17:01:15.75 All dwarf controllers running firmware version 3.75 @17:01:15.91 Cleanly exited at 17:00:10CEST07-May-19 @17:01:15.94 Loading /home/esp/esp2/mission/stdMERwithoutESPready.rb mission @17:01:15.96 AFV.select :carrierSolution @17:01:16.06 delay 2 seconds @17:01:17.45 Session Begun @17:01:18.09
AS.seek 0.5ml @17:01:47.22 delay 1 second @17:01:48.24 AFV.select :holdingCoil @17:01:48.34 ATV.dial :extSrc @17:01:49.84 AS.reconfigure ASslow1 @17:01:49.90 AS.seek 0.53ml @17:01:56.20 delay 5 seconds @17:02:01.28 AS.reconfigure ASslow2 @17:02:01.34 AS.seek 0.54ml @17:02:11.70 delay 20 seconds @17:02:31.77 AS.reconfigure ASslow1 @17:02:31.83 AS.seek 0.57ml @17:02:38.19 delay 5 seconds @17:02:43.27 AS.reconfigure ASslow2 @17:02:43.33 AS.seek 0.58ml @17:02:53.75 delay 20 seconds @17:03:13.83 AS.reconfigure ASslow1 @17:03:13.89 AS.seek 0.61ml @17:03:20.20 delay 5 seconds @17:03:25.28 AS.reconfigure ASslow2 @17:03:25.34 AS.seek 0.62ml @17:03:35.59 delay 20 seconds @17:03:55.66 AS.reconfigure ASslow1 @17:03:55.73 AS.seek 0.65ml @17:04:02.05 delay 5 seconds @17:04:07.13 AS.reconfigure ASslow2 @17:04:07.19 AS.seek 0.66ml @17:04:17.50 delay 20 seconds @17:04:37.53 AS.reconfigure ASslow1 @17:04:37.58 AS.seek 0.705ml @17:04:46.98 delay 10 seconds @17:04:57.01 ATV.dial :air1 @17:04:58.75 AS.seek 0.725ml @17:05:03.05 delay 3 seconds @17:05:06.07 ATV.dial :waste @17:05:06.64 AS.reconfigure ASslow0 @17:05:06.70 AS.seek :empty @17:06:20.17 Setting up PCR for Mm -- writing /var/log/esp/Mm19may0717h.pcr @17:06:20.19 Building PCR Slug from extSrc with MM2 mix, PP3 primer @17:06:20.22 Priming with carrier @17:06:20.23 ATV.dial ARV @17:06:21.86 ARV.dial :carrier @17:06:22.20 AS.reconfigure ASnorm @17:06:22.26 AS.seek 0.499ml @17:06:47.86 AFV.select :carrierSolution @17:06:47.98 delay 2 seconds @17:06:50.01 AS.reconfigure ASconfig @17:06:50.07 AS.seek 1ml @17:07:19.20 delay 1 second @17:07:20.22 AFV.select :holdingCoil @17:07:20.32 delay 1 second @17:07:21.34 ATV.dial :waste @17:07:23.03 AS.reconfigure ASnorm @17:07:23.09 AS.seek :empty @17:08:13.86 AFV.select :carrierSolution @17:08:13.97 delay 2 seconds @17:08:16.00 AS.reconfigure ASconfig @17:08:16.05 AS.seek 0.5ml @17:08:45.19 delay 1 second @17:08:46.21 AFV.select :holdingCoil @17:08:46.32 Building Slug @17:08:46.33 ATV.dial :ARV @17:08:48.01 delay 2 seconds @17:08:50.03 ARV.dial :air1 @17:08:50.54 delay 2 seconds @17:08:52.61 AS.reconfigure ASslow2 @17:08:52.67 AS.seek 0.506ml @17:08:58.95 delay 2 seconds @17:09:00.97 ARV.dial :MM2 @17:09:02.47 delay 2 seconds @17:09:04.49 Adding MM2 @17:09:04.57 AS.seek 0.524ml @17:09:23.03 delay 25 seconds @17:09:48.05 ARV.dial :PP3 @17:09:48.45 Adding PP3 @17:09:48.46 delay 2 seconds @17:09:50.54 AS.seek 0.53ml @17:09:56.80 delay 10 seconds @17:10:06.83 ARV.dial :air2 @17:10:07.22 adding ARV air @17:10:07.23 delay 2 seconds @17:10:09.31 AS.seek 0.536ml @17:10:15.84 delay 5 seconds @17:10:20.86 ARV.dial :carrier @17:10:22.16 delay 7 seconds @17:10:29.24 AS.seek 0.594ml @17:11:27.89 delay 5 seconds @17:11:32.91 ATV.dial :extSrc @17:11:33.48 delay 2 seconds @17:11:35.55 AS.seek 0.6ml @17:11:41.81 delay 4 seconds @17:11:45.83 ATV.dial ARV @17:11:46.39 delay 2 seconds @17:11:48.41 ARV.dial :carrier @17:11:48.42 delay 2 seconds @17:11:50.50 AS.seek 0.65ml @17:12:40.98 delay 4 seconds @17:12:45.00 ATV.dial PCR @17:12:45.78 ASV.dial :waste1 @17:12:45.87 ARV.dial :air1 @17:12:46.26 Positioning Slug by 0.324ml @17:12:46.35 AS.seek 0.326ml @17:13:08.91 -> exit @17:13:08.93 Session Ended @17:18:12.39 delay 5 seconds @17:18:17.41 ATV.dial ARV @17:18:18.19 ASV.dial :PCRlock @17:18:18.58 delay 5 seconds @17:18:23.60 Running Mm Profile60C350 thermocycling @17:18:23.62 PCR.program Profile60C350 @17:18:23.74 Profile60C350 should complete in < 1:48:05 @17:18:23.75 PCR.run 2:20:06.25 @18:42:00.23 PCR.off @18:42:02.15 PCR for Mm completed -- closed /var/log/esp/Mm19may0717h.pcr @18:42:02.21 Cleaning PCR @18:42:02.22 AFV.select :carrierSolution @18:42:02.31 delay 2 seconds @18:42:04.34 AS.reconfigure ASconfig @18:42:04.38 AS.seek 0.5ml @18:42:14.69 delay 1 second @18:42:15.71 AFV.select :holdingCoil @18:42:15.80 ASV.dial :waste1 @18:42:16.19 ATV.dial :air3 @18:42:17.48 AS.reconfigure ASslow1 @18:42:17.52 AS.seek 0.51ml @18:42:19.80 delay 2 seconds @18:42:21.81 ATV.dial :bleach @18:42:22.42 AS.seek 0.81ml @18:43:23.08 delay 3 seconds @18:43:26.10 ATV.dial :air3 @18:43:26.64 delay 3 seconds @18:43:29.71 AS.seek 0.835ml @18:43:35.02 delay 3 seconds @18:43:38.04 ATV.dial :bleach @18:43:38.65 AS.seek 0.82ml @18:43:41.94 delay 3 seconds @18:43:44.96 ARV.dial :waste @18:43:45.56 delay 3 seconds @18:43:48.58 ATV.dial :ARV @18:43:49.56 AS.reconfigure ASconfig @18:43:49.61 AS.seek 0.47ml @18:44:10.03 AFV.select :carrierSolution @18:44:10.13 delay 2 seconds @18:44:12.16 AS.seek 1ml @18:44:42.89 delay 1 second @18:44:43.91 AFV.select :holdingCoil @18:44:44.00 delay 1 second @18:44:45.03 AS.reconfigure ASfast @18:44:45.07 AS.seek 0ml @18:45:12.42 AFV.select :carrierSolution @18:45:12.52 delay 2 seconds @18:45:14.55 AS.reconfigure ASconfig @18:45:14.59 AS.seek 1ml @18:46:12.67 delay 1 second @18:46:13.75 AFV.select :holdingCoil @18:46:13.85 delay 1 second @18:46:14.88 AS.reconfigure ASfast @18:46:14.92 AS.seek 0ml @18:46:42.26 AFV.select :carrierSolution @18:46:42.36 delay 2 seconds @18:46:44.39 AS.reconfigure ASconfig @18:46:44.43 AS.seek 1ml @18:47:42.17 delay 1 second @18:47:43.19 AFV.select :holdingCoil @18:47:43.29 delay 1 second @18:47:44.32 AS.reconfigure ASfast @18:47:44.36 AS.seek 0ml @18:48:11.72 ARV.dial between carrier and air1 @18:48:12.20 ATV.dial :air3 @18:48:13.48 AS.reconfigure ASslow1 @18:48:13.52 AS.seek 0.01ml @18:48:15.81 delay 2 seconds @18:48:17.83 ATV.dial :bleach @18:48:18.43 AS.seek 0.81ml @18:50:59.58 delay 3 seconds @18:51:02.60 ATV.dial :air3 @18:51:03.14 delay 3 seconds @18:51:06.21 AS.seek 0.835ml @18:51:11.54 delay 3 seconds @18:51:14.56 ATV.dial :bleach @18:51:15.17 AS.seek 0.82ml @18:51:18.47 delay 3 seconds @18:51:21.49 ATV.dial PCR @18:51:21.89 AS.reconfigure ASconfig @18:51:21.93 AS.seek :empty @18:52:09.37 PCR.program :bleachProfile @18:52:09.42 PCR program should complete in < 2:45 @18:52:09.43 PCR.run 10:00 @18:55:05.20 PCR.off @18:55:05.22 AFV.select :carrierSolution @18:55:05.32 delay 2 seconds @18:55:07.35 AS.seek 1ml @18:56:05.17 delay 1 second @18:56:06.19 AFV.select :holdingCoil @18:56:06.29 delay 1 second @18:56:07.32 AS.reconfigure ASfast @18:56:07.36 AS.seek 0ml @18:56:34.72 delay 3 seconds @18:56:37.74 AFV.select :carrierSolution @18:56:37.84 delay 2 seconds @18:56:39.87 AS.reconfigure ASconfig @18:56:39.92 AS.seek 1ml @18:57:37.69 delay 1 second @18:57:38.71 AFV.select :holdingCoil @18:57:38.80 delay 1 second @18:57:39.83 AS.reconfigure ASfast @18:57:39.87 AS.seek 0ml @18:58:07.22 delay 3 seconds @18:58:10.24 AFV.select :carrierSolution @18:58:10.33 delay 2 seconds @18:58:12.36 AS.reconfigure ASconfig @18:58:12.40 AS.seek 1ml @18:59:10.19 delay 1 second @18:59:11.21 AFV.select :holdingCoil @18:59:11.31 delay 1 second @18:59:12.34 AS.reconfigure ASfast @18:59:12.38 AS.seek 0ml @18:59:39.73 delay 3 seconds @18:59:42.75 AFV.select :carrierSolution @18:59:42.85 delay 2 seconds @18:59:44.88 AS.reconfigure ASconfig @18:59:44.93 AS.seek 1ml @19:00:42.67 delay 1 second @19:00:43.69 AFV.select :holdingCoil @19:00:43.79 delay 1 second @19:00:44.82 AS.reconfigure ASfast @19:00:44.86 AS.seek 0ml @19:01:12.20 delay 3 seconds @19:01:15.22 AFV.select :carrierSolution @19:01:15.32 delay 2 seconds @19:01:17.35 AS.reconfigure ASconfig @19:01:17.39 AS.seek 1ml @19:02:15.14 delay 1 second @19:02:16.16 AFV.select :holdingCoil @19:02:16.25 delay 1 second @19:02:17.28 AS.reconfigure ASnorm @19:02:17.32 AS.seek 0ml @19:03:08.06
AFV.select :carrierSolution @19:03:08.16 delay 2 seconds @19:03:10.19 AS.reconfigure ASconfig @19:03:10.23 AS.seek 0.5ml @19:03:39.28 delay 1 second @19:03:40.29 AFV.select :holdingCoil @19:03:40.38 ATV.dial :air1 @19:03:41.88 AS.reconfigure ASslow2 @19:03:41.93 AS.seek 0.506ml @19:03:48.36 ATV.dial :extSrc @19:03:50.06 AS.seek 0.512ml @19:03:56.33 delay 5 seconds @19:04:01.35 ATV.dial :air1 @19:04:03.06 AS.reconfigure ASslow1 @19:04:03.11 AS.seek 0.542ml @19:04:09.40 ATV.dial :waste @19:04:09.95 AS.reconfigure ASconfig @19:04:09.99 AS.seek :empty @19:04:41.62 Setting up PCR for Mm -- writing /var/log/esp/Mm19may0719h.pcr @19:04:41.64 Building PCR Slug from extSrc with MM2 mix, PP3 primer @19:04:41.65 Priming with carrier @19:04:41.66 ATV.dial ARV @19:04:43.14 ARV.dial :carrier @19:04:43.48 AS.reconfigure ASnorm @19:04:43.52 AS.seek 0.499ml @19:05:09.12 AFV.select :carrierSolution @19:05:09.22 delay 2 seconds @19:05:11.25 AS.reconfigure ASconfig @19:05:11.29 AS.seek 1ml @19:05:40.39 delay 1 second @19:05:41.41 AFV.select :holdingCoil @19:05:41.51 delay 1 second @19:05:42.53 ATV.dial :waste @19:05:44.20 AS.reconfigure ASnorm @19:05:44.25 AS.seek :empty @19:06:34.98 AFV.select :carrierSolution @19:06:35.08 delay 2 seconds @19:06:37.11 AS.reconfigure ASconfig @19:06:37.15 AS.seek 0.5ml @19:07:06.25 delay 1 second @19:07:07.27 AFV.select :holdingCoil @19:07:07.38 Building Slug @19:07:07.39 ATV.dial :ARV @19:07:09.05 delay 2 seconds @19:07:11.07 ARV.dial :air1 @19:07:11.48 delay 2 seconds @19:07:13.55 AS.reconfigure ASslow2 @19:07:13.59 AS.seek 0.506ml @19:07:20.14 delay 2 seconds @19:07:22.16 ARV.dial :MM2 @19:07:23.62 delay 2 seconds @19:07:25.64 Adding MM2 @19:07:25.71 AS.seek 0.524ml @19:07:44.55 delay 25 seconds @19:08:09.64 ARV.dial :PP3 @19:08:10.01 Adding PP3 @19:08:10.02 delay 2 seconds @19:08:12.09 AS.seek 0.53ml @19:08:18.42 delay 10 seconds @19:08:28.45 ARV.dial :air2 @19:08:28.84 adding ARV air @19:08:28.85 delay 2 seconds @19:08:30.92 AS.seek 0.536ml @19:08:37.30 delay 5 seconds @19:08:42.32 ARV.dial :carrier @19:08:43.61 delay 7 seconds @19:08:50.70 AS.seek 0.594ml @19:09:49.17 delay 5 seconds @19:09:54.19 ATV.dial :extSrc @19:09:54.73 delay 2 seconds @19:09:56.80 AS.seek 0.6ml @19:10:03.17 delay 4 seconds @19:10:07.19 ATV.dial ARV @19:10:07.73 delay 2 seconds @19:10:09.75 ARV.dial :carrier @19:10:09.76 delay 2 seconds @19:10:11.83 AS.seek 0.65ml @19:11:02.30 delay 4 seconds @19:11:06.32 ATV.dial PCR @19:11:07.09 ASV.dial :waste1 @19:11:07.10 ARV.dial :air1 @19:11:07.51 Positioning Slug by 0.324ml @19:11:07.58 AS.seek 0.326ml @19:16:33.87 delay 5 seconds @19:16:38.89 ATV.dial ARV @19:16:39.67 ASV.dial :PCRlock @19:16:40.06 delay 5 seconds @19:16:45.08 Running Mm Profile60C350 thermocycling @19:16:45.09 PCR.program Profile60C350 @19:16:45.22 Profile60C350 should complete in < 1:48:05 @19:16:45.23 PCR.run 2:20:06.25 @20:40:15.82 PCR.off @20:40:18.13 PCR for Mm completed -- closed /var/log/esp/Mm19may0719h.pcr @20:40:18.19 Cleaning PCR @20:40:18.20 AFV.select :carrierSolution @20:40:18.30 delay 2 seconds @20:40:20.33 AS.reconfigure ASconfig @20:40:20.37 AS.seek 0.5ml @20:40:30.64 delay 1 second @20:40:31.66 AFV.select :holdingCoil @20:40:31.75 ASV.dial :waste1 @20:40:32.14 ATV.dial :air3 @20:40:33.43 AS.reconfigure ASslow1 @20:40:33.47 AS.seek 0.51ml @20:40:35.74 delay 2 seconds @20:40:37.75 ATV.dial :bleach @20:40:38.36 AS.seek 0.81ml @20:41:38.94 delay 3 seconds @20:41:41.96 ATV.dial :air3 @20:41:42.50 delay 3 seconds @20:41:45.57 AS.seek 0.835ml @20:41:50.85 delay 3 seconds @20:41:53.87 ATV.dial :bleach @20:41:54.48 AS.seek 0.82ml @20:41:57.78 delay 3 seconds @20:42:00.80 ARV.dial :waste @20:42:01.41 delay 3 seconds @20:42:04.43 ATV.dial :ARV @20:42:05.34 AS.reconfigure ASconfig @20:42:05.38 AS.seek 0.47ml @20:42:25.80 AFV.select :carrierSolution @20:42:25.90 delay 2 seconds @20:42:27.93 AS.seek 1ml @20:42:58.66 delay 1 second @20:42:59.68 AFV.select :holdingCoil @20:42:59.78 delay 1 second @20:43:00.81 AS.reconfigure ASfast @20:43:00.85 AS.seek 0ml @20:43:28.20 AFV.select :carrierSolution @20:43:28.30 delay 2 seconds @20:43:30.33 AS.reconfigure ASconfig @20:43:30.37 AS.seek 1ml @20:44:28.11 delay 1 second @20:44:29.13 AFV.select :holdingCoil @20:44:29.23 delay 1 second @20:44:30.26 AS.reconfigure ASfast @20:44:30.30 AS.seek 0ml @20:44:57.66 AFV.select :carrierSolution @20:44:57.76 delay 2 seconds @20:44:59.79 AS.reconfigure ASconfig @20:44:59.83 AS.seek 1ml @20:45:57.58 delay 1 second @20:45:58.60 AFV.select :holdingCoil @20:45:58.70 delay 1 second @20:45:59.73 AS.reconfigure ASfast @20:45:59.77 AS.seek 0ml @20:46:27.12 ARV.dial between carrier and air1 @20:46:27.61 ATV.dial :air3 @20:46:28.90 AS.reconfigure ASslow1 @20:46:28.94 AS.seek 0.01ml @20:46:31.30 delay 2 seconds @20:46:33.32 ATV.dial :bleach @20:46:33.93 AS.seek 0.81ml @20:49:15.08 delay 3 seconds @20:49:18.10 ATV.dial :air3 @20:49:18.64 delay 3 seconds @20:49:21.71 AS.seek 0.835ml @20:49:27.01 delay 3 seconds @20:49:30.03 ATV.dial :bleach @20:49:30.64 AS.seek 0.82ml @20:49:33.91 delay 3 seconds @20:49:36.93 ATV.dial PCR @20:49:37.33 AS.reconfigure ASconfig @20:49:37.38 AS.seek :empty @20:50:24.83 PCR.program :bleachProfile @20:50:24.87 PCR program should complete in < 2:45 @20:50:24.88 PCR.run 10:00 @20:53:20.65 PCR.off @20:53:20.68 AFV.select :carrierSolution @20:53:20.77 delay 2 seconds @20:53:22.80 AS.seek 1ml @20:54:20.62 delay 1 second @20:54:21.64 AFV.select :holdingCoil @20:54:21.74 delay 1 second @20:54:22.77 AS.reconfigure ASfast @20:54:22.81 AS.seek 0ml @20:54:50.20 delay 3 seconds @20:54:53.22 AFV.select :carrierSolution @20:54:53.32 delay 2 seconds @20:54:55.35 AS.reconfigure ASconfig @20:54:55.40 AS.seek 1ml @20:55:53.16 delay 1 second @20:55:54.18 AFV.select :holdingCoil @20:55:54.28 delay 1 second @20:55:55.31 AS.reconfigure ASfast @20:55:55.35 AS.seek 0ml @20:56:22.70 delay 3 seconds @20:56:25.72 AFV.select :carrierSolution @20:56:25.82 delay 2 seconds @20:56:27.85 AS.reconfigure ASconfig @20:56:27.90 AS.seek 1ml @20:57:25.69 delay 1 second @20:57:26.71 AFV.select :holdingCoil @20:57:26.81 delay 1 second @20:57:27.84 AS.reconfigure ASfast @20:57:27.88 AS.seek 0ml @20:57:55.23 delay 3 seconds @20:57:58.25 AFV.select :carrierSolution @20:57:58.35 delay 2 seconds @20:58:00.38 AS.reconfigure ASconfig @20:58:00.43 AS.seek 1ml @20:58:58.17 delay 1 second @20:58:59.19 AFV.select :holdingCoil @20:58:59.29 delay 1 second @20:59:00.32 AS.reconfigure ASfast @20:59:00.36 AS.seek 0ml @20:59:27.70 delay 3 seconds @20:59:30.72 AFV.select :carrierSolution @20:59:30.81 delay 2 seconds @20:59:32.84 AS.reconfigure ASconfig @20:59:32.89 AS.seek 1ml @21:00:30.69 delay 1 second @21:00:31.71 AFV.select :holdingCoil @21:00:31.80 delay 1 second @21:00:32.83 AS.reconfigure ASnorm @21:00:32.87 AS.seek 0ml @21:01:23.57
AFV.select :carrierSolution @21:01:23.67 delay 2 seconds @21:01:25.70 AS.reconfigure ASconfig @21:01:25.74 AS.seek 0.5ml @21:01:54.81 delay 1 second @21:01:55.83 AFV.select :holdingCoil @21:01:55.92 ATV.dial :air1 @21:01:57.84 AS.reconfigure ASslow2 @21:01:57.88 AS.seek 0.51ml @21:02:08.23 delay 5 seconds @21:02:13.25 ATV.dial :extSrc @21:02:14.97 AS.seek 0.53ml @21:02:35.39 delay 10 seconds @21:02:45.42 ATV.dial :air1 @21:02:47.14 AS.reconfigure ASslow1 @21:02:47.18 AS.seek 0.56ml @21:02:53.48 ATV.dial :waste @21:02:54.03 AS.reconfigure ASconfig @21:02:54.07 AS.seek :empty @21:03:26.75 Setting up PCR for Mm -- writing /var/log/esp/Mm19may0721h.pcr @21:03:26.77 Building PCR Slug from extSrc with MM2 mix, PP3 primer @21:03:26.79 Priming with carrier ATV.dial ARV @21:03:28.28 ARV.dial :carrier @21:03:28.61 AS.reconfigure ASnorm @21:03:28.65 AS.seek 0.499ml @21:03:54.22 AFV.select :carrierSolution @21:03:54.32 delay 2 seconds @21:03:56.35 AS.reconfigure ASconfig @21:03:56.39 AS.seek 1ml @21:04:25.45 delay 1 second @21:04:26.47 AFV.select :holdingCoil @21:04:26.57 delay 1 second @21:04:27.59 ATV.dial :waste @21:04:29.27 AS.reconfigure ASnorm @21:04:29.32 AS.seek :empty @21:05:20.05 AFV.select :carrierSolution @21:05:20.15 delay 2 seconds @21:05:22.18 AS.reconfigure ASconfig @21:05:22.22 AS.seek 0.5ml @21:05:51.31 delay 1 second @21:05:52.33 AFV.select :holdingCoil @21:05:52.43 Building Slug @21:05:52.44 ATV.dial :ARV @21:05:54.09 delay 2 seconds @21:05:56.11 ARV.dial :air1 @21:05:56.50 delay 2 seconds @21:05:58.57 AS.reconfigure ASslow2 @21:05:58.61 AS.seek 0.506ml @21:06:04.89 delay 2 seconds @21:06:06.91 ARV.dial :MM2 @21:06:08.37 delay 2 seconds @21:06:10.39 Adding MM2 @21:06:10.45 AS.seek 0.524ml @21:06:28.95 delay 25 seconds @21:06:53.97 ARV.dial :PP3 @21:06:54.36 Adding PP3 @21:06:54.37 delay 2 seconds @21:06:56.44 AS.seek 0.53ml @21:07:02.67 delay 10 seconds @21:07:12.70 ARV.dial :air2 @21:07:13.08 adding ARV air @21:07:13.09 delay 2 seconds @21:07:15.16 AS.seek 0.536ml @21:07:21.58 delay 5 seconds @21:07:26.60 ARV.dial :carrier @21:07:27.87 delay 7 seconds @21:07:34.95 AS.seek 0.594ml @21:08:33.59 delay 5 seconds @21:08:38.61 ATV.dial :extSrc @21:08:39.17 delay 2 seconds @21:08:41.24 AS.seek 0.6ml @21:08:47.55 delay 4 seconds @21:08:51.57 ATV.dial ARV @21:08:52.11 delay 2 seconds @21:08:54.13 ARV.dial :carrier @21:08:54.14 delay 2 seconds @21:08:56.21 AS.seek 0.65ml @21:09:47.03 delay 4 seconds @21:09:51.05 ATV.dial PCR @21:09:51.81 ASV.dial :waste1 @21:09:51.82 ARV.dial :air1 @21:09:52.22 Positioning Slug by 0.324ml @21:09:52.29 AS.seek 0.326ml @21:15:18.27 delay 5 seconds @21:15:23.29 ATV.dial ARV @21:15:24.06 ASV.dial :PCRlock @21:15:24.45 delay 5 seconds @21:15:29.47 Running Mm Profile60C350 thermocycling @21:15:29.48 PCR.program Profile60C350 @21:15:29.62 Profile60C350 should complete in < 1:48:05 @21:15:29.63 PCR.run 2:20:06.25 @22:38:48.25 PCR.off @22:38:50.22 PCR for Mm completed -- closed /var/log/esp/Mm19may0721h.pcr @22:38:50.28 Cleaning PCR @22:38:50.29 AFV.select :carrierSolution @22:38:50.38 delay 2 seconds @22:38:52.41 AS.reconfigure ASconfig @22:38:52.45 AS.seek 0.5ml @22:39:02.74 delay 1 second @22:39:03.76 AFV.select :holdingCoil @22:39:03.86 ASV.dial :waste1 @22:39:04.25 ATV.dial :air3 @22:39:05.55 AS.reconfigure ASslow1 @22:39:05.59 AS.seek 0.51ml @22:39:07.89 delay 2 seconds @22:39:09.91 ATV.dial :bleach @22:39:10.51 AS.seek 0.81ml @22:40:11.08 delay 3 seconds @22:40:14.10 ATV.dial :air3 @22:40:14.64 delay 3 seconds @22:40:17.71 AS.seek 0.835ml @22:40:23.03 delay 3 seconds @22:40:26.05 ATV.dial :bleach @22:40:26.66 AS.seek 0.82ml @22:40:29.88 delay 3 seconds @22:40:32.90 ARV.dial :waste @22:40:33.50 delay 3 seconds @22:40:36.52 ATV.dial :ARV @22:40:37.41 AS.reconfigure ASconfig @22:40:37.46 AS.seek 0.47ml @22:40:57.88 AFV.select :carrierSolution @22:40:57.98 delay 2 seconds @22:41:00.01 AS.seek 1ml @22:41:30.74 delay 1 second @22:41:31.76 AFV.select :holdingCoil @22:41:31.86 delay 1 second @22:41:33.22 AS.reconfigure ASfast @22:41:33.31 AS.seek 0ml @22:42:00.66 AFV.select :carrierSolution @22:42:00.76 delay 2 seconds @22:42:02.79 AS.reconfigure ASconfig @22:42:02.83 AS.seek 1ml @22:43:00.61 delay 1 second @22:43:01.63 AFV.select :holdingCoil @22:43:01.73 delay 1 second @22:43:02.76 AS.reconfigure ASfast @22:43:02.80 AS.seek 0ml @22:43:30.17 AFV.select :carrierSolution @22:43:30.27 delay 2 seconds @22:43:32.30 AS.reconfigure ASconfig @22:43:32.34 AS.seek 1ml @22:44:30.10 delay 1 second @22:44:31.12 AFV.select :holdingCoil @22:44:31.22 delay 1 second @22:44:32.25 AS.reconfigure ASfast @22:44:32.29 AS.seek 0ml @22:44:59.66 ARV.dial between carrier and air1 @22:45:00.14 ATV.dial :air3 @22:45:01.44 AS.reconfigure ASslow1 @22:45:01.48 AS.seek 0.01ml @22:45:03.77 delay 2 seconds @22:45:05.79 ATV.dial :bleach @22:45:06.39 AS.seek 0.81ml @22:47:47.52 delay 3 seconds @22:47:50.54 ATV.dial :air3 @22:47:51.08 delay 3 seconds @22:47:54.15 AS.seek 0.835ml @22:47:59.44 delay 3 seconds @22:48:02.46 ATV.dial :bleach @22:48:03.06 AS.seek 0.82ml @22:48:06.35 delay 3 seconds @22:48:09.37 ATV.dial PCR @22:48:09.78 AS.reconfigure ASconfig @22:48:09.82 AS.seek :empty @22:48:57.30 PCR.program :bleachProfile @22:48:57.35 PCR program should complete in < 2:45 @22:48:57.36 PCR.run 10:00 @22:51:53.13 PCR.off @22:51:53.16 AFV.select :carrierSolution @22:51:53.26 delay 2 seconds @22:51:55.29 AS.seek 1ml @22:52:53.11 delay 1 second @22:52:54.13 AFV.select :holdingCoil @22:52:54.23 delay 1 second @22:52:55.26 AS.reconfigure ASfast @22:52:55.30 AS.seek 0ml @22:53:22.64 delay 3 seconds @22:53:25.66 AFV.select :carrierSolution @22:53:25.76 delay 2 seconds @22:53:27.79 AS.reconfigure ASconfig @22:53:27.83 AS.seek 1ml @22:54:25.58 delay 1 second @22:54:26.60 AFV.select :holdingCoil @22:54:26.70 delay 1 second @22:54:27.73 AS.reconfigure ASfast @22:54:27.77 AS.seek 0ml @22:54:55.13 delay 3 seconds @22:54:58.15 AFV.select :carrierSolution @22:54:58.25 delay 2 seconds @22:55:00.28 AS.reconfigure ASconfig @22:55:00.32 AS.seek 1ml @22:55:58.11 delay 1 second @22:55:59.13 AFV.select :holdingCoil @22:55:59.23 delay 1 second @22:56:00.26 AS.reconfigure ASfast @22:56:00.30 AS.seek 0ml @22:56:27.64 delay 3 seconds @22:56:30.66 AFV.select :carrierSolution @22:56:30.76 delay 2 seconds @22:56:32.79 AS.reconfigure ASconfig @22:56:32.83 AS.seek 1ml @22:57:30.56 delay 1 second @22:57:31.58 AFV.select :holdingCoil @22:57:31.68 delay 1 second @22:57:32.71 AS.reconfigure ASfast @22:57:32.75 AS.seek 0ml @22:58:00.10 delay 3 seconds @22:58:03.12 AFV.select :carrierSolution @22:58:03.22 delay 2 seconds @22:58:05.25 AS.reconfigure ASconfig @22:58:05.29 AS.seek 1ml @22:59:03.11 delay 1 second @22:59:04.13 AFV.select :holdingCoil @22:59:04.23 delay 1 second @22:59:05.26 AS.reconfigure ASnorm @22:59:05.30 AS.seek 0ml @22:59:56.00
AFV.select :carrierSolution @22:59:56.10 delay 2 seconds @22:59:58.13 AS.reconfigure ASconfig @22:59:58.17 AS.seek 0.5ml @23:00:27.22 delay 1 second @23:00:28.24 AFV.select :holdingCoil @23:00:28.34 ATV.dial :air1 @23:00:29.85 AS.reconfigure ASslow2 @23:00:29.90 AS.seek 0.506ml @23:00:36.10 ATV.dial :extSrc @23:00:37.81 AS.seek 0.512ml @23:00:43.97 delay 5 seconds @23:00:48.99 ATV.dial :air1 @23:00:50.71 AS.reconfigure ASslow1 @23:00:50.75 AS.seek 0.542ml @23:00:57.04 ATV.dial :waste @23:00:57.56 AS.reconfigure ASconfig @23:00:57.60 AS.seek :empty @23:01:29.26 Setting up PCR for Mm -- writing /var/log/esp/Mm19may0723h.pcr @23:01:29.28 Building PCR Slug from extSrc with MM2 mix, PP3 primer @23:01:29.29 Priming with carrier @23:01:29.30 ATV.dial ARV @23:01:30.80 ARV.dial :carrier @23:01:31.14 AS.reconfigure ASnorm @23:01:31.18 AS.seek 0.499ml @23:01:56.75 AFV.select :carrierSolution @23:01:56.85 delay 2 seconds @23:01:58.88 AS.reconfigure ASconfig @23:01:58.92 AS.seek 1ml @23:02:27.97 delay 1 second @23:02:28.99 AFV.select :holdingCoil @23:02:29.09 delay 1 second @23:02:30.11 ATV.dial :waste @23:02:31.78 AS.reconfigure ASnorm @23:02:31.83 AS.seek :empty @23:03:22.56 AFV.select :carrierSolution @23:03:22.66 delay 2 seconds @23:03:24.69 AS.reconfigure ASconfig @23:03:24.73 AS.seek 0.5ml @23:03:53.85 delay 1 second @23:03:54.87 AFV.select :holdingCoil @23:03:54.97 Building Slug @23:03:54.98 ATV.dial :ARV @23:03:56.64 delay 2 seconds @23:03:58.66 ARV.dial :air1 @23:03:59.06 delay 2 seconds @23:04:01.13 AS.reconfigure ASslow2 @23:04:01.18 AS.seek 0.506ml @23:04:07.58 delay 2 seconds @23:04:09.60 ARV.dial :MM2 @23:04:11.06 delay 2 seconds @23:04:13.08 Adding MM2 @23:04:13.15 AS.seek 0.524ml @23:04:31.55 delay 25 seconds @23:04:56.57 ARV.dial :PP3 @23:04:56.96 Adding PP3 @23:04:56.97 delay 2 seconds @23:04:59.04 AS.seek 0.53ml @23:05:05.47 delay 10 seconds @23:05:15.50 ARV.dial :air2 @23:05:15.88 adding ARV air @23:05:15.89 delay 2 seconds @23:05:17.96 AS.seek 0.536ml @23:05:24.28 delay 5 seconds @23:05:29.30 ARV.dial :carrier @23:05:31.08 delay 7 seconds @23:05:38.16 AS.seek 0.594ml @23:06:36.64 delay 5 seconds @23:06:41.66 ATV.dial :extSrc @23:06:42.22 delay 2 seconds @23:06:44.29 AS.seek 0.6ml @23:06:50.52 delay 4 seconds @23:06:54.54 ATV.dial ARV @23:06:55.08 delay 2 seconds @23:06:57.10 ARV.dial :carrier @23:06:57.11 delay 2 seconds @23:06:59.18 AS.seek 0.65ml @23:07:49.85 delay 4 seconds @23:07:53.87 ATV.dial PCR @23:07:54.64 ASV.dial :waste1 @23:07:54.65 ARV.dial :air1 @23:07:55.04 Positioning Slug by 0.324ml @23:07:55.11 AS.seek 0.326ml @23:13:21.25 delay 5 seconds @23:13:26.27 ATV.dial ARV @23:13:27.05 ASV.dial :PCRlock @23:13:27.44 delay 5 seconds @23:13:32.46 Running Mm Profile60C350 thermocycling @23:13:32.47 PCR.program Profile60C350 @23:13:32.60 Profile60C350 should complete in < 1:48:05 @23:13:32.61 PCR.run 2:20:06.25 @00:36:45.31CEST08-May-19 PCR.off @00:36:47.29 PCR for Mm completed -- closed /var/log/esp/Mm19may0723h.pcr @00:36:47.35 Cleaning PCR @00:36:47.36 AFV.select :carrierSolution @00:36:47.46 delay 2 seconds @00:36:49.49 AS.reconfigure ASconfig @00:36:49.53 AS.seek 0.5ml @00:36:59.83 delay 1 second @00:37:00.85 AFV.select :holdingCoil @00:37:00.94 ASV.dial :waste1 @00:37:01.33 ATV.dial :air3 @00:37:02.63 AS.reconfigure ASslow1 @00:37:02.67 AS.seek 0.51ml @00:37:04.97 delay 2 seconds @00:37:06.99 ATV.dial :bleach @00:37:07.60 AS.seek 0.81ml @00:38:08.18 delay 3 seconds @00:38:11.20 ATV.dial :air3 @00:38:11.75 delay 3 seconds @00:38:14.82 AS.seek 0.835ml @00:38:20.11 delay 3 seconds @00:38:23.13 ATV.dial :bleach @00:38:23.74 AS.seek 0.82ml @00:38:27.05 delay 3 seconds @00:38:30.07 ARV.dial :waste @00:38:30.68 delay 3 seconds @00:38:33.70 ATV.dial :ARV @00:38:34.59 AS.reconfigure ASconfig @00:38:34.64 AS.seek 0.47ml @00:38:55.07 AFV.select :carrierSolution @00:38:55.17 delay 2 seconds @00:38:57.20 AS.seek 1ml @00:39:27.93 delay 1 second @00:39:28.95 AFV.select :holdingCoil @00:39:29.05 delay 1 second @00:39:30.08 AS.reconfigure ASfast @00:39:30.12 AS.seek 0ml @00:39:57.47 AFV.select :carrierSolution @00:39:57.57 delay 2 seconds @00:39:59.60 AS.reconfigure ASconfig @00:39:59.64 AS.seek 1ml @00:40:57.39 delay 1 second @00:40:58.41 AFV.select :holdingCoil @00:40:58.51 delay 1 second @00:40:59.54 AS.reconfigure ASfast @00:40:59.58 AS.seek 0ml @00:41:26.91 AFV.select :carrierSolution @00:41:27.01 delay 2 seconds @00:41:29.43 AS.reconfigure ASconfig @00:41:29.47 AS.seek 1ml @00:42:27.22 delay 1 second @00:42:28.24 AFV.select :holdingCoil @00:42:28.34 delay 1 second @00:42:29.37 AS.reconfigure ASfast @00:42:29.41 AS.seek 0ml @00:42:56.75 ARV.dial between carrier and air1 @00:42:57.24 ATV.dial :air3 @00:42:58.53 AS.reconfigure ASslow1 @00:42:58.57 AS.seek 0.01ml @00:43:00.86 delay 2 seconds @00:43:02.88 ATV.dial :bleach @00:43:03.49 AS.seek 0.81ml @00:45:44.61 delay 3 seconds @00:45:47.63 ATV.dial :air3 @00:45:48.18 delay 3 seconds @00:45:51.25 AS.seek 0.835ml @00:45:56.55 delay 3 seconds @00:45:59.57 ATV.dial :bleach @00:46:00.17 AS.seek 0.82ml @00:46:03.46 delay 3 seconds @00:46:06.48 ATV.dial PCR @00:46:06.88 AS.reconfigure ASconfig @00:46:06.93 AS.seek :empty @00:46:54.39 PCR.program :bleachProfile @00:46:54.43 PCR program should complete in < 2:45 @00:46:54.44 PCR.run 10:00 @00:49:50.21 PCR.off @00:49:50.24 AFV.select :carrierSolution @00:49:50.34 delay 2 seconds @00:49:52.37 AS.seek 1ml @00:50:50.19 delay 1 second @00:50:51.21 AFV.select :holdingCoil @00:50:51.31 delay 1 second @00:50:52.34 AS.reconfigure ASfast @00:50:52.38 AS.seek 0ml @00:51:19.72 delay 3 seconds @00:51:22.74 AFV.select :carrierSolution @00:51:22.84 delay 2 seconds @00:51:24.87 AS.reconfigure ASconfig @00:51:24.92 AS.seek 1ml @00:52:22.68 delay 1 second @00:52:23.70 AFV.select :holdingCoil @00:52:23.80 delay 1 second @00:52:24.83 AS.reconfigure ASfast @00:52:24.87 AS.seek 0ml @00:52:52.22 delay 3 seconds @00:52:55.24 AFV.select :carrierSolution @00:52:55.34 delay 2 seconds @00:52:57.37 AS.reconfigure ASconfig @00:52:57.41 AS.seek 1ml @00:53:55.14 delay 1 second @00:53:56.16 AFV.select :holdingCoil @00:53:56.26 delay 1 second @00:53:57.29 AS.reconfigure ASfast @00:53:57.33 AS.seek 0ml @00:54:24.68 delay 3 seconds @00:54:27.70 AFV.select :carrierSolution @00:54:27.80 delay 2 seconds @00:54:29.83 AS.reconfigure ASconfig @00:54:29.88 AS.seek 1ml @00:55:27.61 delay 1 second @00:55:28.63 AFV.select :holdingCoil @00:55:28.73 delay 1 second @00:55:29.76 AS.reconfigure ASfast @00:55:29.80 AS.seek 0ml @00:55:57.15 delay 3 seconds @00:56:00.17 AFV.select :carrierSolution @00:56:00.27 delay 2 seconds @00:56:02.30 AS.reconfigure ASconfig @00:56:02.34 AS.seek 1ml @00:57:00.08 delay 1 second @00:57:01.10 AFV.select :holdingCoil @00:57:01.20 delay 1 second @00:57:02.23 AS.reconfigure ASnorm @00:57:02.27 AS.seek 0ml @00:57:52.95
AFV.select :carrierSolution @00:57:53.05 delay 2 seconds @00:57:55.08 AS.reconfigure ASconfig @00:57:55.12 AS.seek 0.5ml @00:58:24.18 delay 1 second @00:58:25.20 AFV.select :holdingCoil @00:58:25.30 ATV.dial :air1 @00:58:26.79 AS.reconfigure ASslow1 @00:58:26.84 AS.seek 0.52ml @00:58:31.10 delay 5 seconds @00:58:36.12 ATV.dial :extSrc @00:58:37.83 AS.reconfigure ASslow2 @00:58:37.87 AS.seek 0.545ml @00:59:03.21 delay 10 seconds @00:59:13.24 ATV.dial :air1 @00:59:14.95 AS.reconfigure ASslow1 @00:59:14.99 AS.seek 0.575ml @00:59:21.30 ATV.dial :waste @00:59:21.85 AS.reconfigure ASconfig @00:59:21.89 AS.seek :empty @00:59:55.41 Setting up PCR for Mm -- writing /var/log/esp/Mm19may0800h.pcr @00:59:55.43 Building PCR Slug from extSrc with MM2 mix, PP3 primer @00:59:55.45 Priming with carrier ATV.dial ARV @00:59:56.94 ARV.dial :carrier @00:59:57.66 AS.reconfigure ASnorm @00:59:57.70 AS.seek 0.499ml @01:00:23.27 AFV.select :carrierSolution @01:00:23.37 delay 2 seconds @01:00:25.40 AS.reconfigure ASconfig @01:00:25.44 AS.seek 1ml @01:00:54.50 delay 1 second @01:00:55.52 AFV.select :holdingCoil @01:00:55.62 delay 1 second @01:00:56.64 ATV.dial :waste @01:00:58.33 AS.reconfigure ASnorm @01:00:58.38 AS.seek :empty @01:01:49.13 AFV.select :carrierSolution @01:01:49.23 delay 2 seconds @01:01:51.26 AS.reconfigure ASconfig @01:01:51.30 AS.seek 0.5ml @01:02:20.36 delay 1 second @01:02:21.38 AFV.select :holdingCoil @01:02:21.48 Building Slug @01:02:21.49 ATV.dial :ARV @01:02:23.15 delay 2 seconds @01:02:25.17 ARV.dial :air1 @01:02:25.56 delay 2 seconds @01:02:27.63 AS.reconfigure ASslow2 @01:02:27.68 AS.seek 0.506ml @01:02:33.99 delay 2 seconds @01:02:36.01 ARV.dial :MM2 @01:02:37.47 delay 2 seconds @01:02:39.49 Adding MM2 @01:02:39.55 AS.seek 0.524ml @01:02:57.96 delay 25 seconds @01:03:22.98 ARV.dial :PP3 @01:03:23.36 Adding PP3 @01:03:23.37 delay 2 seconds @01:03:25.44 AS.seek 0.53ml @01:03:32.02 delay 10 seconds @01:03:42.05 ARV.dial :air2 @01:03:42.43 adding ARV air @01:03:42.44 delay 2 seconds @01:03:44.51 AS.seek 0.536ml @01:03:50.91 delay 5 seconds @01:03:55.93 ARV.dial :carrier @01:03:57.21 delay 7 seconds @01:04:04.29 AS.seek 0.594ml @01:05:02.83 delay 5 seconds @01:05:07.85 ATV.dial :extSrc @01:05:08.41 delay 2 seconds @01:05:10.48 AS.seek 0.6ml @01:05:16.93 delay 4 seconds @01:05:20.95 ATV.dial ARV @01:05:21.50 delay 2 seconds @01:05:23.52 ARV.dial :carrier @01:05:23.53 delay 2 seconds @01:05:25.60 AS.seek 0.65ml @01:06:16.11 delay 4 seconds @01:06:20.13 ATV.dial PCR @01:06:20.90 ASV.dial :waste1 @01:06:20.91 ARV.dial :air1 @01:06:21.30 Positioning Slug by 0.324ml @01:06:21.37 AS.seek 0.326ml @01:11:47.43 delay 5 seconds @01:11:52.45 ATV.dial ARV @01:11:53.22 ASV.dial :PCRlock @01:11:53.61 delay 5 seconds @01:11:58.63 Running Mm Profile60C350 thermocycling @01:11:58.64 PCR.program Profile60C350 @01:11:58.77 Profile60C350 should complete in < 1:48:05 @01:11:58.78 PCR.run 2:20:06.25 @02:35:11.45 PCR.off @02:35:13.42 PCR for Mm completed -- closed /var/log/esp/Mm19may0800h.pcr @02:35:13.48 Cleaning PCR @02:35:13.49 AFV.select :carrierSolution @02:35:13.59 delay 2 seconds @02:35:15.62 AS.reconfigure ASconfig @02:35:15.66 AS.seek 0.5ml @02:35:25.99 delay 1 second @02:35:27.01 AFV.select :holdingCoil @02:35:27.11 ASV.dial :waste1 @02:35:27.51 ATV.dial :air3 @02:35:28.80 AS.reconfigure ASslow1 @02:35:28.84 AS.seek 0.51ml @02:35:31.13 delay 2 seconds @02:35:33.15 ATV.dial :bleach @02:35:33.75 AS.seek 0.81ml @02:36:34.32 delay 3 seconds @02:36:37.34 ATV.dial :air3 @02:36:37.88 delay 3 seconds @02:36:40.95 AS.seek 0.835ml @02:36:46.26 delay 3 seconds @02:36:49.28 ATV.dial :bleach @02:36:49.88 AS.seek 0.82ml @02:36:53.18 delay 3 seconds @02:36:56.20 ARV.dial :waste @02:36:56.80 delay 3 seconds @02:36:59.82 ATV.dial :ARV @02:37:00.71 AS.reconfigure ASconfig @02:37:00.76 AS.seek 0.47ml @02:37:21.16 AFV.select :carrierSolution @02:37:21.26 delay 2 seconds @02:37:23.29 AS.seek 1ml @02:37:54.07 delay 1 second @02:37:55.09 AFV.select :holdingCoil @02:37:55.19 delay 1 second @02:37:56.22 AS.reconfigure ASfast @02:37:56.26 AS.seek 0ml @02:38:23.63 AFV.select :carrierSolution @02:38:23.73 delay 2 seconds @02:38:25.76 AS.reconfigure ASconfig @02:38:25.80 AS.seek 1ml @02:39:23.55 delay 1 second @02:39:24.57 AFV.select :holdingCoil @02:39:24.67 delay 1 second @02:39:25.70 AS.reconfigure ASfast @02:39:25.74 AS.seek 0ml @02:39:53.10 AFV.select :carrierSolution @02:39:53.20 delay 2 seconds @02:39:55.23 AS.reconfigure ASconfig @02:39:55.27 AS.seek 1ml @02:40:53.01 delay 1 second @02:40:54.03 AFV.select :holdingCoil @02:40:54.13 delay 1 second @02:40:55.16 AS.reconfigure ASfast @02:40:55.20 AS.seek 0ml @02:41:22.54 ARV.dial between carrier and air1 @02:41:23.02 ATV.dial :air3 @02:41:24.71 AS.reconfigure ASslow1 @02:41:24.75 AS.seek 0.01ml @02:41:27.07 delay 2 seconds @02:41:29.09 ATV.dial :bleach @02:41:29.69 AS.seek 0.81ml @02:44:10.82 delay 3 seconds @02:44:13.84 ATV.dial :air3 @02:44:14.38 delay 3 seconds @02:44:17.45 AS.seek 0.835ml @02:44:22.77 delay 3 seconds @02:44:25.79 ATV.dial :bleach @02:44:26.40 AS.seek 0.82ml @02:44:29.71 delay 3 seconds @02:44:32.73 ATV.dial PCR @02:44:33.13 AS.reconfigure ASconfig @02:44:33.18 AS.seek :empty @02:45:20.63 PCR.program :bleachProfile @02:45:20.68 PCR program should complete in < 2:45 @02:45:20.69 PCR.run 10:00 @02:48:16.46 PCR.off @02:48:16.49 AFV.select :carrierSolution @02:48:16.59 delay 2 seconds @02:48:18.62 AS.seek 1ml @02:49:16.49 delay 1 second @02:49:17.51 AFV.select :holdingCoil @02:49:17.61 delay 1 second @02:49:18.64 AS.reconfigure ASfast @02:49:18.68 AS.seek 0ml @02:49:46.04 delay 3 seconds @02:49:49.06 AFV.select :carrierSolution @02:49:49.16 delay 2 seconds @02:49:51.19 AS.reconfigure ASconfig @02:49:51.23 AS.seek 1ml @02:50:48.99 delay 1 second @02:50:50.01 AFV.select :holdingCoil @02:50:50.11 delay 1 second @02:50:51.14 AS.reconfigure ASfast @02:50:51.18 AS.seek 0ml @02:51:18.54 delay 3 seconds @02:51:21.56 AFV.select :carrierSolution @02:51:21.66 delay 2 seconds @02:51:23.69 AS.reconfigure ASconfig @02:51:23.73 AS.seek 1ml @02:52:21.48 delay 1 second @02:52:22.50 AFV.select :holdingCoil @02:52:22.60 delay 1 second @02:52:23.63 AS.reconfigure ASfast @02:52:23.67 AS.seek 0ml @02:52:51.02 delay 3 seconds @02:52:54.04 AFV.select :carrierSolution @02:52:54.14 delay 2 seconds @02:52:56.17 AS.reconfigure ASconfig @02:52:56.21 AS.seek 1ml @02:53:53.96 delay 1 second @02:53:54.98 AFV.select :holdingCoil @02:53:55.08 delay 1 second @02:53:56.11 AS.reconfigure ASfast @02:53:56.15 AS.seek 0ml @02:54:23.51 delay 3 seconds @02:54:26.53 AFV.select :carrierSolution @02:54:26.63 delay 2 seconds @02:54:28.66 AS.reconfigure ASconfig @02:54:28.71 AS.seek 1ml @02:55:26.48 delay 1 second @02:55:27.50 AFV.select :holdingCoil @02:55:27.60 delay 1 second @02:55:28.63 AS.reconfigure ASnorm @02:55:28.67 AS.seek 0ml @02:56:19.39
AFV.select :carrierSolution @02:56:19.49 delay 2 seconds @02:56:21.52 AS.reconfigure ASconfig @02:56:21.56 AS.seek 0.5ml @02:56:50.63 delay 1 second @02:56:51.65 AFV.select :holdingCoil @02:56:51.75 ATV.dial :air1 @02:56:53.27 AS.reconfigure ASslow2 @02:56:53.31 AS.seek 0.506ml @02:56:59.74 ATV.dial :extSrc @02:57:01.46 AS.seek 0.512ml @02:57:08.05 delay 5 seconds @02:57:13.07 ATV.dial :air1 @02:57:14.80 AS.reconfigure ASslow1 @02:57:14.84 AS.seek 0.542ml @02:57:21.18 ATV.dial :waste @02:57:21.71 AS.reconfigure ASconfig @02:57:21.75 AS.seek :empty @02:57:53.38 Setting up PCR for Mm -- writing /var/log/esp/Mm19may0802h.pcr @02:57:53.41 Building PCR Slug from extSrc with MM2 mix, PP3 primer @02:57:53.42 Priming with carrier ATV.dial ARV @02:57:54.91 ARV.dial :carrier @02:57:55.26 AS.reconfigure ASnorm @02:57:55.30 AS.seek 0.499ml @02:58:20.88 AFV.select :carrierSolution @02:58:20.98 delay 2 seconds @02:58:23.01 AS.reconfigure ASconfig @02:58:23.05 AS.seek 1ml @02:58:52.10 delay 1 second @02:58:53.12 AFV.select :holdingCoil @02:58:53.22 delay 1 second @02:58:54.57 ATV.dial :waste @02:58:56.30 AS.reconfigure ASnorm @02:58:56.35 AS.seek :empty @02:59:47.09 AFV.select :carrierSolution @02:59:47.19 delay 2 seconds @02:59:49.22 AS.reconfigure ASconfig @02:59:49.26 AS.seek 0.5ml @03:00:18.34 delay 1 second @03:00:19.36 AFV.select :holdingCoil @03:00:19.46 Building Slug @03:00:19.47 ATV.dial :ARV @03:00:21.13 delay 2 seconds @03:00:23.15 ARV.dial :air1 @03:00:23.55 delay 2 seconds @03:00:25.62 AS.reconfigure ASslow2 @03:00:25.67 AS.seek 0.506ml @03:00:31.88 delay 2 seconds @03:00:33.90 ARV.dial :MM2 @03:00:35.37 delay 2 seconds @03:00:37.39 Adding MM2 @03:00:37.45 AS.seek 0.524ml @03:00:55.88 delay 25 seconds @03:01:20.90 ARV.dial :PP3 @03:01:21.29 Adding PP3 @03:01:21.30 delay 2 seconds @03:01:23.37 AS.seek 0.53ml @03:01:29.93 delay 10 seconds @03:01:39.96 ARV.dial :air2 @03:01:40.34 adding ARV air @03:01:40.35 delay 2 seconds @03:01:42.42 AS.seek 0.536ml @03:01:48.66 delay 5 seconds @03:01:53.68 ARV.dial :carrier @03:01:54.96 delay 7 seconds @03:02:02.04 AS.seek 0.594ml @03:03:00.59 delay 5 seconds @03:03:05.61 ATV.dial :extSrc @03:03:06.16 delay 2 seconds @03:03:08.23 AS.seek 0.6ml @03:03:14.63 delay 4 seconds @03:03:18.65 ATV.dial ARV @03:03:19.20 delay 2 seconds @03:03:21.22 ARV.dial :carrier @03:03:21.23 delay 2 seconds @03:03:23.30 AS.seek 0.65ml @03:04:13.70 delay 4 seconds @03:04:17.72 ATV.dial PCR @03:04:18.49 ASV.dial :waste1 @03:04:18.50 ARV.dial :air1 @03:04:18.91 Positioning Slug by 0.324ml @03:04:18.98 AS.seek 0.326ml @03:09:45.32 delay 5 seconds @03:09:50.34 ATV.dial ARV @03:09:51.12 ASV.dial :PCRlock @03:09:51.60 delay 5 seconds @03:09:56.62 Running Mm Profile60C350 thermocycling @03:09:56.63 PCR.program Profile60C350 @03:09:56.76 Profile60C350 should complete in < 1:48:05 @03:09:56.77 PCR.run 2:20:06.25 @04:33:09.80 PCR.off @04:33:11.78 PCR for Mm completed -- closed /var/log/esp/Mm19may0802h.pcr @04:33:11.84 Cleaning PCR @04:33:11.85 AFV.select :carrierSolution @04:33:11.95 delay 2 seconds @04:33:13.98 AS.reconfigure ASconfig @04:33:14.02 AS.seek 0.5ml @04:33:24.34 delay 1 second @04:33:25.36 AFV.select :holdingCoil @04:33:25.46 ASV.dial :waste1 @04:33:25.84 ATV.dial :air3 @04:33:27.13 AS.reconfigure ASslow1 @04:33:27.17 AS.seek 0.51ml @04:33:29.43 delay 2 seconds @04:33:31.45 ATV.dial :bleach @04:33:32.06 AS.seek 0.81ml @04:34:32.64 delay 3 seconds @04:34:35.66 ATV.dial :air3 @04:34:36.21 delay 3 seconds @04:34:39.28 AS.seek 0.835ml @04:34:44.57 delay 3 seconds @04:34:47.59 ATV.dial :bleach @04:34:48.20 AS.seek 0.82ml @04:34:51.48 delay 3 seconds @04:34:54.50 ARV.dial :waste @04:34:55.10 delay 3 seconds @04:34:58.12 ATV.dial :ARV @04:34:59.01 AS.reconfigure ASconfig @04:34:59.06 AS.seek 0.47ml @04:35:19.48 AFV.select :carrierSolution @04:35:19.58 delay 2 seconds @04:35:21.61 AS.seek 1ml @04:35:52.39 delay 1 second @04:35:53.41 AFV.select :holdingCoil @04:35:53.51 delay 1 second @04:35:54.54 AS.reconfigure ASfast @04:35:54.58 AS.seek 0ml @04:36:21.93 AFV.select :carrierSolution @04:36:22.03 delay 2 seconds @04:36:24.06 AS.reconfigure ASconfig @04:36:24.10 AS.seek 1ml @04:37:21.85 delay 1 second @04:37:22.87 AFV.select :holdingCoil @04:37:22.97 delay 1 second @04:37:24.00 AS.reconfigure ASfast @04:37:24.04 AS.seek 0ml @04:37:51.37 AFV.select :carrierSolution @04:37:51.47 delay 2 seconds @04:37:53.50 AS.reconfigure ASconfig @04:37:53.54 AS.seek 1ml @04:38:51.32 delay 1 second @04:38:52.34 AFV.select :holdingCoil @04:38:52.44 delay 1 second @04:38:53.47 AS.reconfigure ASfast @04:38:53.51 AS.seek 0ml @04:39:20.85 ARV.dial between carrier and air1 @04:39:21.34 ATV.dial :air3 @04:39:22.63 AS.reconfigure ASslow1 @04:39:22.67 AS.seek 0.01ml @04:39:24.96 delay 2 seconds @04:39:26.98 ATV.dial :bleach @04:39:27.59 AS.seek 0.81ml @04:42:08.71 delay 3 seconds @04:42:12.12 ATV.dial :air3 @04:42:12.67 delay 3 seconds @04:42:15.74 AS.seek 0.835ml @04:42:21.05 delay 3 seconds @04:42:24.07 ATV.dial :bleach @04:42:24.70 AS.seek 0.82ml @04:42:28.01 delay 3 seconds @04:42:31.03 ATV.dial PCR @04:42:31.42 AS.reconfigure ASconfig @04:42:31.46 AS.seek :empty @04:43:18.93 PCR.program :bleachProfile @04:43:18.98 PCR program should complete in < 2:45 @04:43:18.99 PCR.run 10:00 @04:46:14.76 PCR.off @04:46:14.79 AFV.select :carrierSolution @04:46:14.89 delay 2 seconds @04:46:16.92 AS.seek 1ml @04:47:14.75 delay 1 second @04:47:15.77 AFV.select :holdingCoil @04:47:15.87 delay 1 second @04:47:16.90 AS.reconfigure ASfast @04:47:16.94 AS.seek 0ml @04:47:44.29 delay 3 seconds @04:47:47.31 AFV.select :carrierSolution @04:47:47.41 delay 2 seconds @04:47:49.44 AS.reconfigure ASconfig @04:47:49.49 AS.seek 1ml @04:48:47.23 delay 1 second @04:48:48.25 AFV.select :holdingCoil @04:48:48.35 delay 1 second @04:48:49.38 AS.reconfigure ASfast @04:48:49.42 AS.seek 0ml @04:49:16.78 delay 3 seconds @04:49:19.80 AFV.select :carrierSolution @04:49:19.90 delay 2 seconds @04:49:21.93 AS.reconfigure ASconfig @04:49:21.97 AS.seek 1ml @04:50:19.73 delay 1 second @04:50:20.75 AFV.select :holdingCoil @04:50:20.85 delay 1 second @04:50:21.88 AS.reconfigure ASfast @04:50:21.92 AS.seek 0ml @04:50:49.26 delay 3 seconds @04:50:52.28 AFV.select :carrierSolution @04:50:52.38 delay 2 seconds @04:50:54.41 AS.reconfigure ASconfig @04:50:54.46 AS.seek 1ml @04:51:52.20 delay 1 second @04:51:53.22 AFV.select :holdingCoil @04:51:53.32 delay 1 second @04:51:54.35 AS.reconfigure ASfast @04:51:54.39 AS.seek 0ml @04:52:21.75 delay 3 seconds @04:52:24.77 AFV.select :carrierSolution @04:52:24.87 delay 2 seconds @04:52:26.90 AS.reconfigure ASconfig @04:52:26.94 AS.seek 1ml @04:53:24.73 delay 1 second @04:53:25.75 AFV.select :holdingCoil @04:53:25.84 delay 1 second @04:53:26.87 AS.reconfigure ASnorm @04:53:26.91 AS.seek 0ml @04:54:17.65
AFV.select :carrierSolution @04:54:17.75 delay 2 seconds @04:54:19.78 AS.reconfigure ASconfig @04:54:19.82 AS.seek 0.5ml @04:54:48.87 delay 1 second @04:54:49.89 AFV.select :holdingCoil @04:54:49.99 ATV.dial :air1 @04:54:51.49 AS.reconfigure ASslow1 @04:54:51.53 AS.seek 0.52ml @04:54:55.87 delay 5 seconds @04:55:00.89 ATV.dial :extSrc @04:55:02.59 AS.reconfigure ASslow2 @04:55:02.64 AS.seek 0.538ml @04:55:21.12 delay 10 seconds @04:55:31.14 ATV.dial :air1 @04:55:32.84 AS.reconfigure ASslow1 @04:55:32.89 AS.seek 0.568ml @04:55:39.20 ATV.dial :waste @04:55:39.75 AS.reconfigure ASconfig @04:55:39.79 AS.seek :empty @04:56:12.79 deconEluateRecoveryLine extSrc @04:56:12.80 ATV.dial :waste @04:56:12.81 AS.reconfigure ASnorm @04:56:12.85 AS.seek 0ml @04:56:13.23 ATV.dial :air3 @04:56:13.90 AS.reconfigure ASslow1 @04:56:13.94 AS.seek 0.01ml @04:56:16.20 delay 2 seconds @04:56:18.22 ATV.dial :bleach @04:56:18.68 AS.seek 0.31ml @04:57:19.26 delay 3 seconds @04:57:22.28 ATV.dial :air3 @04:57:22.84 delay 3 seconds @04:57:25.91 AS.seek 0.335ml @04:57:31.19 delay 3 seconds @04:57:34.21 ATV.dial :bleach @04:57:34.82 AS.seek 0.32ml @04:57:38.11 delay 3 seconds @04:57:41.13 ATV.dial :extSrc @04:57:41.77 AS.seek 0ml @04:58:46.36 AFV.select :carrierSolution @04:58:46.87 delay 2 seconds @04:58:48.90 AS.reconfigure ASconfig @04:58:48.94 AS.seek 0.5ml @04:59:17.98 delay 1 second @04:59:19.00 AFV.select :holdingCoil @04:59:19.10 ATV.dial :extSrc @04:59:19.12 AS.reconfigure ASslow1 @04:59:19.16 AS.seek 0ml @05:01:00.01 AFV.select :carrierSolution @05:01:00.11 delay 2 seconds @05:01:02.14 AS.reconfigure ASconfig @05:01:02.18 AS.seek 0.5ml @05:01:31.20 delay 1 second @05:01:32.22 AFV.select :holdingCoil @05:01:32.32 ATV.dial :extSrc @05:01:32.34 AS.reconfigure ASslow1 @05:01:32.38 AS.seek 0ml @05:03:13.20 AFV.select :carrierSolution @05:03:13.30 delay 2 seconds @05:03:15.33 AS.reconfigure ASconfig @05:03:15.37 AS.seek 0.5ml @05:03:44.40 delay 1 second @05:03:45.42 AFV.select :holdingCoil @05:03:45.52 ATV.dial :extSrc @05:03:45.54 AS.reconfigure ASslow1 @05:03:45.58 AS.seek 0ml @05:05:26.40 AFV.select :carrierSolution @05:05:26.50 delay 2 seconds @05:05:28.53 AS.reconfigure ASconfig @05:05:28.57 AS.seek 0.5ml @05:05:57.61 delay 1 second @05:05:58.63 AFV.select :holdingCoil @05:05:58.73 ATV.dial :extSrc @05:05:58.75 AS.reconfigure ASslow1 @05:05:58.79 AS.seek 0ml @05:07:39.61 ATV.dial :air2 @05:07:40.95 AS.reconfigure ASslow0 @05:07:40.99 AS.seek 0.5ml @05:08:31.57 ATV.dial :extSrc @05:08:33.07 AS.seek 0.02ml @05:09:21.65 delay 1 second @05:09:22.67 ATV.dial :waste @05:09:23.96 AS.reconfigure ASconfig @05:09:24.00 AS.seek :empty @05:09:25.46 deconEluateRecoveryLine extSrc @05:09:25.47 ATV.dial :waste @05:09:25.48 AS.reconfigure ASnorm @05:09:25.53 AS.seek 0ml @05:09:25.87 ATV.dial :air3 @05:09:26.72 AS.reconfigure ASslow1 @05:09:26.76 AS.seek 0.01ml @05:09:29.03 delay 2 seconds @05:09:31.05 ATV.dial :bleach @05:09:31.51 AS.seek 0.31ml @05:10:32.11 delay 3 seconds @05:10:35.13 ATV.dial :air3 @05:10:35.70 delay 3 seconds @05:10:38.77 AS.seek 0.335ml @05:10:44.08 delay 3 seconds @05:10:47.10 ATV.dial :bleach @05:10:47.71 AS.seek 0.32ml @05:10:51.00 delay 3 seconds @05:10:54.02 ATV.dial :extSrc @05:10:54.66 AS.seek 0ml @05:11:59.21 AFV.select :carrierSolution @05:11:59.31 delay 2 seconds @05:12:01.34 AS.reconfigure ASconfig @05:12:01.38 AS.seek 0.5ml @05:12:30.46 delay 1 second @05:12:31.48 AFV.select :holdingCoil @05:12:31.58 ATV.dial :extSrc @05:12:31.60 AS.reconfigure ASslow1 @05:12:31.64 AS.seek 0ml @05:14:12.45 AFV.select :carrierSolution @05:14:12.55 delay 2 seconds @05:14:14.58 AS.reconfigure ASconfig @05:14:14.62 AS.seek 0.5ml @05:14:43.64 delay 1 second @05:14:44.66 AFV.select :holdingCoil @05:14:44.76 ATV.dial :extSrc @05:14:44.78 AS.reconfigure ASslow1 @05:14:44.82 AS.seek 0ml @05:16:25.65 AFV.select :carrierSolution @05:16:25.75 delay 2 seconds @05:16:27.78 AS.reconfigure ASconfig @05:16:27.82 AS.seek 0.5ml @05:16:56.84 delay 1 second @05:16:57.86 AFV.select :holdingCoil @05:16:57.96 ATV.dial :extSrc @05:16:57.98 AS.reconfigure ASslow1 @05:16:58.02 AS.seek 0ml @05:18:38.84 AFV.select :carrierSolution @05:18:38.94 delay 2 seconds @05:18:40.97 AS.reconfigure ASconfig @05:18:41.01 AS.seek 0.5ml @05:19:10.01 delay 1 second @05:19:11.03 AFV.select :holdingCoil @05:19:11.12 ATV.dial :extSrc @05:19:11.14 AS.reconfigure ASslow1 @05:19:11.18 AS.seek 0ml @05:20:52.01 ATV.dial :air2 @05:20:53.35 AS.reconfigure ASslow0 @05:20:53.39 AS.seek 0.5ml @05:21:44.01 ATV.dial :extSrc @05:21:45.51 AS.seek 0.02ml @05:22:34.11 delay 1 second @05:22:35.13 ATV.dial :waste @05:22:36.43 AS.reconfigure ASconfig @05:22:36.47 AS.seek :empty @05:22:37.97 deconEluateRecoveryLine extSrc @05:22:37.98 ATV.dial :waste @05:22:37.99 AS.reconfigure ASnorm @05:22:38.03 AS.seek 0ml @05:22:38.37 ATV.dial :air3 @05:22:39.22 AS.reconfigure ASslow1 @05:22:39.63 AS.seek 0.01ml @05:22:41.93 delay 2 seconds @05:22:43.95 ATV.dial :bleach @05:22:44.40 AS.seek 0.31ml @05:23:45.00 delay 3 seconds @05:23:48.02 ATV.dial :air3 @05:23:48.57 delay 3 seconds @05:23:51.64 AS.seek 0.335ml @05:23:56.96 delay 3 seconds @05:23:59.98 ATV.dial :bleach @05:24:00.59 AS.seek 0.32ml @05:24:03.89 delay 3 seconds @05:24:06.91 ATV.dial :extSrc @05:24:07.54 AS.seek 0ml @05:25:12.14 AFV.select :carrierSolution @05:25:12.24 delay 2 seconds @05:25:14.27 AS.reconfigure ASconfig @05:25:14.31 AS.seek 0.5ml @05:25:43.36 delay 1 second @05:25:44.38 AFV.select :holdingCoil @05:25:44.48 ATV.dial :extSrc @05:25:44.50 AS.reconfigure ASslow1 @05:25:44.54 AS.seek 0ml @05:27:25.36 AFV.select :carrierSolution @05:27:25.46 delay 2 seconds @05:27:27.49 AS.reconfigure ASconfig @05:27:27.53 AS.seek 0.5ml @05:27:56.56 delay 1 second @05:27:57.58 AFV.select :holdingCoil @05:27:57.68 ATV.dial :extSrc @05:27:57.70 AS.reconfigure ASslow1 @05:27:57.74 AS.seek 0ml @05:29:38.61 AFV.select :carrierSolution @05:29:38.71 delay 2 seconds @05:29:40.74 AS.reconfigure ASconfig @05:29:40.78 AS.seek 0.5ml @05:30:09.81 delay 1 second @05:30:10.83 AFV.select :holdingCoil @05:30:10.93 ATV.dial :extSrc @05:30:10.95 AS.reconfigure ASslow1 @05:30:10.99 AS.seek 0ml @05:31:51.82 AFV.select :carrierSolution @05:31:51.92 delay 2 seconds @05:31:53.95 AS.reconfigure ASconfig @05:31:53.99 AS.seek 0.5ml @05:32:23.03 delay 1 second @05:32:24.05 AFV.select :holdingCoil @05:32:24.15 ATV.dial :extSrc @05:32:24.17 AS.reconfigure ASslow1 @05:32:24.21 AS.seek 0ml @05:34:05.01 ATV.dial :air2 @05:34:06.35 AS.reconfigure ASslow0 @05:34:06.39 AS.seek 0.5ml @05:34:56.93 ATV.dial :extSrc @05:34:58.43 AS.seek 0.02ml @05:35:47.00 delay 1 second @05:35:48.02 ATV.dial :waste @05:35:49.33 AS.reconfigure ASconfig @05:35:49.37 AS.seek :empty @16:08:38.65CEST08-May-19
ESP2 v3.30 I2C Gateway v3.06 for core bus @16:08:40.32 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @16:08:40.59 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @16:08:45.21 Polling Can every 10:00 @16:08:47.39 SPE.coast @16:08:47.44 SPE.configure SPEconfig @16:08:47.52 AS.reconfigure ASconfig @16:08:47.56 AS.hold @16:08:47.60 AS.configure ASconfig @16:08:47.69 SS.reconfigure SSconfig @16:08:47.73 SS.hold @16:08:47.79 SS.configure SSconfig @16:08:47.88 CC.configure CCconfig @16:08:47.95 CS.reconfigure CSconfig @16:08:47.99 CS.hold @16:08:48.04 CS.configure CSconfig @16:08:48.12 PC.configure PCconfig @16:08:48.70 PS.configure PSconfig @16:08:48.96 Forearm.reconfigure LooseForearm @16:08:49.03 Forearm.hold @16:08:49.13 Forearm.configure LooseForearm @16:08:49.25 Elbow.reconfigure LooseElbow @16:08:49.29 Elbow.hold @16:08:49.33 Elbow.configure LooseElbow @16:08:49.43 SC.configure SCloose @16:08:49.51 SE.configure SEconfig @16:08:49.55 CH.coast @16:08:49.61 CH.configure CHconfig @16:08:49.64 PH.coast @16:08:49.69 PH.configure PHconfig @16:08:49.72 PCR.off @16:08:49.75 PCR.pidtemp= 50,0.1,0,300,25,25 @16:08:49.77 PCR.caltemp= 2.8814,0.0372,0 @16:08:49.81 All dwarf controllers running firmware version 3.75 @16:08:49.97 Cleanly exited at 16:07:10CEST08-May-19 @16:08:50.00 Loading /home/esp/esp2/mission/stdMERwithoutESPready.rb mission @16:08:50.02 AFV.select :carrierSolution @16:08:50.12 delay 2 seconds @16:08:51.20 Session Begun @16:08:52.15
AS.seek 0.5ml @16:09:21.20 delay 1 second @16:09:22.22 AFV.select :holdingCoil @16:09:22.34 ATV.dial :extSrc @16:09:22.79 AS.reconfigure ASslow1 @16:09:22.85 AS.seek 0.53ml @16:09:29.15 delay 5 seconds @16:09:34.23 AS.reconfigure ASslow2 @16:09:34.29 AS.seek 0.54ml @16:09:44.80 delay 20 seconds @16:10:04.87 AS.reconfigure ASslow1 @16:10:04.93 AS.seek 0.57ml @16:10:11.30 delay 5 seconds @16:10:16.38 AS.reconfigure ASslow2 @16:10:16.44 AS.seek 0.58ml @16:10:26.65 delay 20 seconds @16:10:46.72 AS.reconfigure ASslow1 @16:10:46.78 AS.seek 0.61ml @16:10:53.09 delay 5 seconds @16:10:58.17 AS.reconfigure ASslow2 @16:10:58.23 AS.seek 0.62ml @16:11:08.44 delay 20 seconds @16:11:28.51 AS.reconfigure ASslow1 @16:11:28.58 AS.seek 0.65ml @16:11:34.90 delay 5 seconds @16:11:39.98 AS.reconfigure ASslow2 @16:11:40.04 AS.seek 0.66ml @16:11:50.31 delay 20 seconds @16:12:10.34 AS.reconfigure ASslow1 @16:12:10.40 AS.seek 0.705ml @16:12:19.78 delay 10 seconds @16:12:29.81 ATV.dial :air1 @16:12:31.54 AS.seek 0.725ml @16:12:35.83 delay 3 seconds @16:12:38.85 ATV.dial :waste @16:12:39.41 AS.reconfigure ASslow0 @16:12:39.46 AS.seek :empty @16:13:52.92 Setting up PCR for Pp -- writing /var/log/esp/Pp19may0816h.pcr @16:13:52.94 Building PCR Slug from extSrc with MM1 mix, PP2 primer @16:13:52.96 Priming with carrier @16:13:52.99 ATV.dial ARV @16:13:54.51 ARV.dial :carrier @16:13:54.86 AS.reconfigure ASnorm @16:13:54.93 AS.seek 0.499ml @16:14:20.47 AFV.select :carrierSolution @16:14:20.59 delay 2 seconds @16:14:22.61 AS.reconfigure ASconfig @16:14:22.68 AS.seek 1ml @16:14:51.73 delay 1 second @16:14:52.75 AFV.select :holdingCoil @16:14:52.86 delay 1 second @16:14:53.87 ATV.dial :waste @16:14:55.59 AS.reconfigure ASnorm @16:14:55.65 AS.seek :empty @16:15:46.40 AFV.select :carrierSolution @16:15:46.52 delay 2 seconds @16:15:48.54 AS.reconfigure ASconfig @16:15:48.60 AS.seek 0.5ml @16:16:17.70 delay 1 second @16:16:18.72 AFV.select :holdingCoil @16:16:18.83 Building Slug @16:16:18.84 ATV.dial :ARV @16:16:20.51 delay 2 seconds @16:16:22.53 ARV.dial :air1 @16:16:22.93 delay 2 seconds @16:16:25.00 AS.reconfigure ASslow2 @16:16:25.06 AS.seek 0.506ml @16:16:31.25 delay 2 seconds @16:16:33.27 ARV.dial :MM1 @16:16:33.89 delay 2 seconds @16:16:35.92 Adding MM1 @16:16:36.00 AS.seek 0.524ml @16:16:54.30 delay 25 seconds @16:17:19.32 ARV.dial :PP2 @16:17:19.72 Adding PP2 @16:17:19.74 delay 2 seconds @16:17:21.82 AS.seek 0.53ml @16:17:28.22 delay 10 seconds @16:17:38.25 ARV.dial :air2 @16:17:38.64 adding ARV air @16:17:38.66 delay 2 seconds @16:17:40.74 AS.seek 0.536ml @16:17:47.03 delay 5 seconds @16:17:52.05 ARV.dial :carrier @16:17:53.33 delay 7 seconds @16:18:00.41 AS.seek 0.594ml @16:18:58.90 delay 5 seconds @16:19:03.92 ATV.dial :extSrc @16:19:04.50 delay 2 seconds @16:19:06.58 AS.seek 0.6ml @16:19:12.84 delay 4 seconds @16:19:16.86 ATV.dial ARV @16:19:17.42 delay 2 seconds @16:19:19.44 ARV.dial :carrier @16:19:19.46 delay 2 seconds @16:19:21.54 AS.seek 0.65ml @16:20:12.05 delay 4 seconds @16:20:16.07 ATV.dial PCR @16:20:16.84 ASV.dial :waste1 @16:20:16.94 ARV.dial :air1 @16:20:17.40 Positioning Slug by 0.324ml @16:20:17.49 AS.seek 0.326ml @16:25:43.61 delay 5 seconds @16:25:48.63 ATV.dial ARV @16:25:49.42 ASV.dial :PCRlock @16:25:49.83 delay 5 seconds @16:25:54.86 Running Pp Profile60C350 thermocycling @16:25:54.87 PCR.program Profile60C350 @16:25:55.02 Profile60C350 should complete in < 1:48:05 @16:25:55.03 PCR.run 2:20:06.25 @17:49:32.49 PCR.off @17:49:34.48 PCR for Pp completed -- closed /var/log/esp/Pp19may0816h.pcr @17:49:34.56 Cleaning PCR @17:49:34.57 AFV.select :carrierSolution @17:49:34.70 delay 2 seconds @17:49:36.72 AS.reconfigure ASconfig @17:49:36.77 AS.seek 0.5ml @17:49:47.08 delay 1 second @17:49:48.10 AFV.select :holdingCoil @17:49:48.20 ASV.dial :waste1 @17:49:48.61 ATV.dial :air3 @17:49:49.92 AS.reconfigure ASslow1 @17:49:49.98 AS.seek 0.51ml @17:49:52.27 delay 2 seconds @17:49:54.29 ATV.dial :bleach @17:49:54.90 AS.seek 0.81ml @17:50:55.51 delay 3 seconds @17:50:58.53 ATV.dial :air3 @17:50:59.09 delay 3 seconds @17:51:02.16 AS.seek 0.835ml @17:51:07.45 delay 3 seconds @17:51:10.47 ATV.dial :bleach @17:51:11.09 AS.seek 0.82ml @17:51:14.36 delay 3 seconds @17:51:17.38 ARV.dial :waste @17:51:18.00 delay 3 seconds @17:51:21.02 ATV.dial :ARV @17:51:21.93 AS.reconfigure ASconfig @17:51:21.98 AS.seek 0.47ml @17:51:42.80 AFV.select :carrierSolution @17:51:42.92 delay 2 seconds @17:51:44.94 AS.seek 1ml @17:52:15.78 delay 1 second @17:52:16.80 AFV.select :holdingCoil @17:52:16.91 delay 1 second @17:52:17.93 AS.reconfigure ASfast @17:52:17.99 AS.seek 0ml @17:52:45.34 AFV.select :carrierSolution @17:52:45.46 delay 2 seconds @17:52:47.48 AS.reconfigure ASconfig @17:52:47.54 AS.seek 1ml @17:53:45.28 delay 1 second @17:53:46.30 AFV.select :holdingCoil @17:53:46.41 delay 1 second @17:53:47.43 AS.reconfigure ASfast @17:53:47.49 AS.seek 0ml @17:54:14.84 AFV.select :carrierSolution @17:54:14.96 delay 2 seconds @17:54:16.98 AS.reconfigure ASconfig @17:54:17.05 AS.seek 1ml @17:55:14.84 delay 1 second @17:55:15.86 AFV.select :holdingCoil @17:55:15.97 delay 1 second @17:55:16.99 AS.reconfigure ASfast @17:55:17.06 AS.seek 0ml @17:55:44.44 ARV.dial between carrier and air1 @17:55:44.95 ATV.dial :air3 @17:55:46.26 AS.reconfigure ASslow1 @17:55:46.32 AS.seek 0.01ml @17:55:48.61 delay 2 seconds @17:55:50.63 ATV.dial :bleach @17:55:51.25 AS.seek 0.81ml @17:58:32.40 delay 3 seconds @17:58:35.42 ATV.dial :air3 @17:58:35.98 delay 3 seconds @17:58:39.06 AS.seek 0.835ml @17:58:44.35 delay 3 seconds @17:58:47.37 ATV.dial :bleach @17:58:47.98 AS.seek 0.82ml @17:58:51.30 delay 3 seconds @17:58:54.32 ATV.dial PCR @17:58:54.76 AS.reconfigure ASconfig @17:58:54.81 AS.seek :empty @17:59:42.28 PCR.program :bleachProfile @17:59:42.34 PCR program should complete in < 2:45 @17:59:42.35 PCR.run 10:00 @18:02:38.13 PCR.off @18:02:38.17 AFV.select :carrierSolution @18:02:38.29 delay 2 seconds @18:02:40.31 AS.seek 1ml @18:03:38.15 delay 1 second @18:03:39.17 AFV.select :holdingCoil @18:03:39.28 delay 1 second @18:03:40.30 AS.reconfigure ASfast @18:03:40.36 AS.seek 0ml @18:04:07.72 delay 3 seconds @18:04:10.74 AFV.select :carrierSolution @18:04:10.85 delay 2 seconds @18:04:12.87 AS.reconfigure ASconfig @18:04:12.94 AS.seek 1ml @18:05:10.69 delay 1 second @18:05:11.71 AFV.select :holdingCoil @18:05:11.82 delay 1 second @18:05:12.84 AS.reconfigure ASfast @18:05:12.89 AS.seek 0ml @18:05:40.25 delay 3 seconds @18:05:43.27 AFV.select :carrierSolution @18:05:43.38 delay 2 seconds @18:05:45.40 AS.reconfigure ASconfig @18:05:45.45 AS.seek 1ml @18:06:43.22 delay 1 second @18:06:44.24 AFV.select :holdingCoil @18:06:44.35 delay 1 second @18:06:45.37 AS.reconfigure ASfast @18:06:45.42 AS.seek 0ml @18:07:12.78 delay 3 seconds @18:07:15.80 AFV.select :carrierSolution @18:07:15.91 delay 2 seconds @18:07:17.93 AS.reconfigure ASconfig @18:07:18.01 AS.seek 1ml @18:08:15.76 delay 1 second @18:08:16.78 AFV.select :holdingCoil @18:08:16.90 delay 1 second @18:08:17.92 AS.reconfigure ASfast @18:08:17.97 AS.seek 0ml @18:08:45.34 delay 3 seconds @18:08:48.36 AFV.select :carrierSolution @18:08:48.48 delay 2 seconds @18:08:50.50 AS.reconfigure ASconfig @18:08:50.56 AS.seek 1ml @18:09:48.34 delay 1 second @18:09:49.36 AFV.select :holdingCoil @18:09:49.47 delay 1 second @18:09:50.49 AS.reconfigure ASnorm @18:09:50.54 AS.seek 0ml @18:10:41.27
AFV.select :carrierSolution @18:10:41.38 delay 2 seconds @18:10:43.40 AS.reconfigure ASconfig @18:10:43.45 AS.seek 0.5ml @18:11:12.51 delay 1 second @18:11:13.53 AFV.select :holdingCoil @18:11:13.65 ATV.dial :air1 @18:11:15.17 AS.reconfigure ASslow2 @18:11:15.22 AS.seek 0.506ml @18:11:21.59 ATV.dial :extSrc @18:11:23.32 AS.seek 0.512ml @18:11:29.70 delay 5 seconds @18:11:34.72 ATV.dial :air1 @18:11:36.46 AS.reconfigure ASslow1 @18:11:36.52 AS.seek 0.542ml @18:11:42.81 ATV.dial :waste @18:11:43.37 AS.reconfigure ASconfig @18:11:43.43 AS.seek :empty @18:12:15.10 Setting up PCR for Pp -- writing /var/log/esp/Pp19may0818h.pcr @18:12:15.12 Building PCR Slug from extSrc with MM1 mix, PP2 primer @18:12:15.15 Priming with carrier @18:12:15.17 ATV.dial ARV @18:12:16.69 ARV.dial :carrier @18:12:17.04 AS.reconfigure ASnorm @18:12:17.10 AS.seek 0.499ml @18:12:42.69 AFV.select :carrierSolution @18:12:42.81 delay 2 seconds @18:12:44.83 AS.reconfigure ASconfig @18:12:44.90 AS.seek 1ml @18:13:13.98 delay 1 second @18:13:15.00 AFV.select :holdingCoil @18:13:15.11 delay 1 second @18:13:16.12 ATV.dial :waste @18:13:17.82 AS.reconfigure ASnorm @18:13:17.88 AS.seek :empty @18:14:08.61 AFV.select :carrierSolution @18:14:08.73 delay 2 seconds @18:14:10.75 AS.reconfigure ASconfig @18:14:10.81 AS.seek 0.5ml @18:14:39.92 delay 1 second @18:14:40.94 AFV.select :holdingCoil @18:14:41.05 Building Slug @18:14:41.06 ATV.dial :ARV @18:14:42.73 delay 2 seconds @18:14:44.75 ARV.dial :air1 @18:14:45.58 delay 2 seconds @18:14:47.65 AS.reconfigure ASslow2 @18:14:47.71 AS.seek 0.506ml @18:14:53.95 delay 2 seconds @18:14:55.97 ARV.dial :MM1 @18:14:56.65 delay 2 seconds @18:14:58.68 Adding MM1 @18:14:58.76 AS.seek 0.524ml @18:15:17.22 delay 25 seconds @18:15:42.24 ARV.dial :PP2 @18:15:42.64 Adding PP2 @18:15:42.66 delay 2 seconds @18:15:44.74 AS.seek 0.53ml @18:15:51.01 delay 10 seconds @18:16:01.04 ARV.dial :air2 @18:16:01.42 adding ARV air @18:16:01.44 delay 2 seconds @18:16:03.52 AS.seek 0.536ml @18:16:10.00 delay 5 seconds @18:16:15.02 ARV.dial :carrier @18:16:16.30 delay 7 seconds @18:16:23.39 AS.seek 0.594ml @18:17:22.09 delay 5 seconds @18:17:27.11 ATV.dial :extSrc @18:17:27.67 delay 2 seconds @18:17:29.75 AS.seek 0.6ml @18:17:36.06 delay 4 seconds @18:17:40.08 ATV.dial ARV @18:17:40.64 delay 2 seconds @18:17:42.66 ARV.dial :carrier @18:17:42.68 delay 2 seconds @18:17:44.76 AS.seek 0.65ml @18:18:35.20 delay 4 seconds @18:18:39.22 ATV.dial PCR @18:18:40.00 ASV.dial :waste1 @18:18:40.01 ARV.dial :air1 @18:18:40.53 Positioning Slug by 0.324ml @18:18:40.62 AS.seek 0.326ml @18:24:06.76 delay 5 seconds @18:24:11.78 ATV.dial ARV @18:24:12.58 ASV.dial :PCRlock @18:24:12.98 delay 5 seconds @18:24:18.01 Running Pp Profile60C350 thermocycling @18:24:18.02 PCR.program Profile60C350 @18:24:18.18 Profile60C350 should complete in < 1:48:05 @18:24:18.19 PCR.run 2:20:06.25 @19:47:42.75 PCR.off @19:47:44.81 PCR for Pp completed -- closed /var/log/esp/Pp19may0818h.pcr @19:47:44.89 Cleaning PCR @19:47:44.90 AFV.select :carrierSolution @19:47:45.02 delay 2 seconds @19:47:47.04 AS.reconfigure ASconfig @19:47:47.10 AS.seek 0.5ml @19:47:57.39 delay 1 second @19:47:58.41 AFV.select :holdingCoil @19:47:58.51 ASV.dial :waste1 @19:47:58.92 ATV.dial :air3 @19:48:00.23 AS.reconfigure ASslow1 @19:48:00.29 AS.seek 0.51ml @19:48:02.51 delay 2 seconds @19:48:04.53 ATV.dial :bleach @19:48:05.15 AS.seek 0.81ml @19:49:05.78 delay 3 seconds @19:49:08.80 ATV.dial :air3 @19:49:09.36 delay 3 seconds @19:49:12.44 AS.seek 0.835ml @19:49:17.77 delay 3 seconds @19:49:20.79 ATV.dial :bleach @19:49:21.42 AS.seek 0.82ml @19:49:24.70 delay 3 seconds @19:49:27.72 ARV.dial :waste @19:49:28.33 delay 3 seconds @19:49:31.35 ATV.dial :ARV @19:49:32.26 AS.reconfigure ASconfig @19:49:32.32 AS.seek 0.47ml @19:49:52.75 AFV.select :carrierSolution @19:49:52.87 delay 2 seconds @19:49:54.89 AS.seek 1ml @19:50:25.64 delay 1 second @19:50:26.66 AFV.select :holdingCoil @19:50:26.77 delay 1 second @19:50:27.79 AS.reconfigure ASfast @19:50:27.86 AS.seek 0ml @19:50:55.22 AFV.select :carrierSolution @19:50:55.34 delay 2 seconds @19:50:57.36 AS.reconfigure ASconfig @19:50:57.42 AS.seek 1ml @19:51:55.17 delay 1 second @19:51:56.19 AFV.select :holdingCoil @19:51:56.30 delay 1 second @19:51:57.32 AS.reconfigure ASfast @19:51:57.38 AS.seek 0ml @19:52:24.73 AFV.select :carrierSolution @19:52:24.85 delay 2 seconds @19:52:26.87 AS.reconfigure ASconfig @19:52:26.92 AS.seek 1ml @19:53:24.69 delay 1 second @19:53:25.71 AFV.select :holdingCoil @19:53:25.82 delay 1 second @19:53:26.84 AS.reconfigure ASfast @19:53:26.90 AS.seek 0ml @19:53:54.26 ARV.dial between carrier and air1 @19:53:54.76 ATV.dial :air3 @19:53:56.07 AS.reconfigure ASslow1 @19:53:56.13 AS.seek 0.01ml @19:53:58.44 delay 2 seconds @19:54:00.46 ATV.dial :bleach @19:54:01.07 AS.seek 0.81ml @19:56:42.23 delay 3 seconds @19:56:45.25 ATV.dial :air3 @19:56:45.81 delay 3 seconds @19:56:48.88 AS.seek 0.835ml @19:56:54.20 delay 3 seconds @19:56:57.22 ATV.dial :bleach @19:56:57.84 AS.seek 0.82ml @19:57:01.16 delay 3 seconds @19:57:04.18 ATV.dial PCR @19:57:04.59 AS.reconfigure ASconfig @19:57:04.65 AS.seek :empty @19:57:52.14 PCR.program :bleachProfile @19:57:52.20 PCR program should complete in < 2:45 @19:57:52.21 PCR.run 10:00 @20:00:47.99 PCR.off @20:00:48.03 AFV.select :carrierSolution @20:00:48.15 delay 2 seconds @20:00:50.17 AS.seek 1ml @20:01:48.01 delay 1 second @20:01:49.03 AFV.select :holdingCoil @20:01:49.15 delay 1 second @20:01:50.17 AS.reconfigure ASfast @20:01:50.22 AS.seek 0ml @20:02:17.58 delay 3 seconds @20:02:20.60 AFV.select :carrierSolution @20:02:20.71 delay 2 seconds @20:02:22.73 AS.reconfigure ASconfig @20:02:22.80 AS.seek 1ml @20:03:20.55 delay 1 second @20:03:21.57 AFV.select :holdingCoil @20:03:21.68 delay 1 second @20:03:22.70 AS.reconfigure ASfast @20:03:22.75 AS.seek 0ml @20:03:50.11 delay 3 seconds @20:03:53.13 AFV.select :carrierSolution @20:03:53.24 delay 2 seconds @20:03:55.26 AS.reconfigure ASconfig @20:03:55.32 AS.seek 1ml @20:04:53.06 delay 1 second @20:04:54.08 AFV.select :holdingCoil @20:04:54.19 delay 1 second @20:04:55.21 AS.reconfigure ASfast @20:04:55.26 AS.seek 0ml @20:05:22.59 delay 3 seconds @20:05:25.61 AFV.select :carrierSolution @20:05:25.72 delay 2 seconds @20:05:27.74 AS.reconfigure ASconfig @20:05:27.81 AS.seek 1ml @20:06:25.61 delay 1 second @20:06:26.63 AFV.select :holdingCoil @20:06:26.74 delay 1 second @20:06:27.76 AS.reconfigure ASfast @20:06:27.81 AS.seek 0ml @20:06:55.17 delay 3 seconds @20:06:58.19 AFV.select :carrierSolution @20:06:58.30 delay 2 seconds @20:07:00.32 AS.reconfigure ASconfig @20:07:00.38 AS.seek 1ml @20:07:58.14 delay 1 second @20:07:59.16 AFV.select :holdingCoil @20:07:59.27 delay 1 second @20:08:00.29 AS.reconfigure ASnorm @20:08:00.34 AS.seek 0ml @20:08:51.50
AFV.select :carrierSolution @20:08:51.61 delay 2 seconds @20:08:53.63 AS.reconfigure ASconfig @20:08:53.68 AS.seek 0.5ml @20:09:22.75 delay 1 second @20:09:23.77 AFV.select :holdingCoil @20:09:23.89 ATV.dial :air1 @20:09:25.40 AS.reconfigure ASslow2 @20:09:25.46 AS.seek 0.51ml @20:09:35.92 delay 5 seconds @20:09:40.95 ATV.dial :extSrc @20:09:42.68 AS.seek 0.53ml @20:10:03.25 delay 10 seconds @20:10:13.28 ATV.dial :air1 @20:10:15.00 AS.reconfigure ASslow1 @20:10:15.06 AS.seek 0.56ml @20:10:21.42 ATV.dial :waste @20:10:21.99 AS.reconfigure ASconfig @20:10:22.04 AS.seek :empty @20:10:54.73 Setting up PCR for Pp -- writing /var/log/esp/Pp19may0820h.pcr @20:10:54.75 Building PCR Slug from extSrc with MM1 mix, PP2 primer @20:10:54.78 Priming with carrier @20:10:54.79 ATV.dial ARV @20:10:56.33 ARV.dial :carrier @20:10:56.67 AS.reconfigure ASnorm @20:10:56.73 AS.seek 0.499ml @20:11:22.30 AFV.select :carrierSolution @20:11:22.42 delay 2 seconds @20:11:24.44 AS.reconfigure ASconfig @20:11:24.51 AS.seek 1ml @20:11:53.59 delay 1 second @20:11:54.61 AFV.select :holdingCoil @20:11:54.72 delay 1 second @20:11:55.73 ATV.dial :waste @20:11:57.45 AS.reconfigure ASnorm @20:11:57.51 AS.seek :empty @20:12:48.26 AFV.select :carrierSolution @20:12:48.38 delay 2 seconds @20:12:50.40 AS.reconfigure ASconfig @20:12:50.47 AS.seek 0.5ml @20:13:19.58 delay 1 second @20:13:20.60 AFV.select :holdingCoil @20:13:20.71 Building Slug @20:13:20.72 ATV.dial :ARV @20:13:22.39 delay 2 seconds @20:13:24.41 ARV.dial :air1 @20:13:24.81 delay 2 seconds @20:13:26.88 AS.reconfigure ASslow2 @20:13:26.94 AS.seek 0.506ml @20:13:33.16 delay 2 seconds @20:13:35.18 ARV.dial :MM1 @20:13:35.78 delay 2 seconds @20:13:37.81 Adding MM1 @20:13:37.89 AS.seek 0.524ml @20:13:56.17 delay 25 seconds @20:14:21.19 ARV.dial :PP2 @20:14:21.58 Adding PP2 @20:14:21.60 delay 2 seconds @20:14:23.68 AS.seek 0.53ml @20:14:29.94 delay 10 seconds @20:14:39.97 ARV.dial :air2 @20:14:40.36 adding ARV air @20:14:40.38 delay 2 seconds @20:14:42.46 AS.seek 0.536ml @20:14:48.92 delay 5 seconds @20:14:53.94 ARV.dial :carrier @20:14:55.22 delay 7 seconds @20:15:02.31 AS.seek 0.594ml @20:16:00.84 delay 5 seconds @20:16:05.86 ATV.dial :extSrc @20:16:06.42 delay 2 seconds @20:16:08.50 AS.seek 0.6ml @20:16:14.78 delay 4 seconds @20:16:18.80 ATV.dial ARV @20:16:19.36 delay 2 seconds @20:16:21.38 ARV.dial :carrier @20:16:21.40 delay 2 seconds @20:16:23.48 AS.seek 0.65ml @20:17:14.14 delay 4 seconds @20:17:18.16 ATV.dial PCR @20:17:18.94 ASV.dial :waste1 @20:17:18.95 ARV.dial :air1 @20:17:19.48 Positioning Slug by 0.324ml @20:17:19.56 AS.seek 0.326ml @20:22:45.70 delay 5 seconds @20:22:50.72 ATV.dial ARV @20:22:51.51 ASV.dial :PCRlock @20:22:51.91 delay 5 seconds @20:22:56.94 Running Pp Profile60C350 thermocycling @20:22:56.95 PCR.program Profile60C350 @20:22:57.11 Profile60C350 should complete in < 1:48:05 @20:22:57.12 PCR.run 2:20:06.25 @21:46:27.19 PCR.off @21:46:29.18 PCR for Pp completed -- closed /var/log/esp/Pp19may0820h.pcr @21:46:29.26 Cleaning PCR @21:46:29.27 AFV.select :carrierSolution @21:46:29.40 delay 2 seconds @21:46:31.42 AS.reconfigure ASconfig @21:46:31.47 AS.seek 0.5ml @21:46:41.80 delay 1 second @21:46:42.82 AFV.select :holdingCoil @21:46:42.92 ASV.dial :waste1 @21:46:43.31 ATV.dial :air3 @21:46:44.64 AS.reconfigure ASslow1 @21:46:44.70 AS.seek 0.51ml @21:46:46.98 delay 2 seconds @21:46:49.00 ATV.dial :bleach @21:46:49.64 AS.seek 0.81ml @21:47:50.25 delay 3 seconds @21:47:53.27 ATV.dial :air3 @21:47:53.83 delay 3 seconds @21:47:56.90 AS.seek 0.835ml @21:48:02.23 delay 3 seconds @21:48:05.25 ATV.dial :bleach @21:48:05.87 AS.seek 0.82ml @21:48:09.17 delay 3 seconds @21:48:12.19 ARV.dial :waste @21:48:12.78 delay 3 seconds @21:48:15.80 ATV.dial :ARV @21:48:17.04 AS.reconfigure ASconfig @21:48:17.15 AS.seek 0.47ml @21:48:37.56 AFV.select :carrierSolution @21:48:37.68 delay 2 seconds @21:48:39.70 AS.seek 1ml @21:49:10.47 delay 1 second @21:49:11.49 AFV.select :holdingCoil @21:49:11.60 delay 1 second @21:49:12.62 AS.reconfigure ASfast @21:49:12.68 AS.seek 0ml @21:49:40.02 AFV.select :carrierSolution @21:49:40.14 delay 2 seconds @21:49:42.16 AS.reconfigure ASconfig @21:49:42.23 AS.seek 1ml @21:50:39.98 delay 1 second @21:50:41.00 AFV.select :holdingCoil @21:50:41.11 delay 1 second @21:50:42.13 AS.reconfigure ASfast @21:50:42.19 AS.seek 0ml @21:51:09.55 AFV.select :carrierSolution @21:51:09.67 delay 2 seconds @21:51:11.69 AS.reconfigure ASconfig @21:51:11.75 AS.seek 1ml @21:52:09.50 delay 1 second @21:52:10.52 AFV.select :holdingCoil @21:52:10.63 delay 1 second @21:52:11.65 AS.reconfigure ASfast @21:52:11.71 AS.seek 0ml @21:52:39.06 ARV.dial between carrier and air1 @21:52:39.56 ATV.dial :air3 @21:52:40.87 AS.reconfigure ASslow1 @21:52:40.93 AS.seek 0.01ml @21:52:43.25 delay 2 seconds @21:52:45.27 ATV.dial :bleach @21:52:45.89 AS.seek 0.81ml @21:55:27.03 delay 3 seconds @21:55:30.05 ATV.dial :air3 @21:55:30.61 delay 3 seconds @21:55:33.68 AS.seek 0.835ml @21:55:38.99 delay 3 seconds @21:55:42.01 ATV.dial :bleach @21:55:42.65 AS.seek 0.82ml @21:55:45.97 delay 3 seconds @21:55:48.99 ATV.dial PCR @21:55:49.42 AS.reconfigure ASconfig @21:55:49.47 AS.seek :empty @21:56:36.95 PCR.program :bleachProfile @21:56:37.01 PCR program should complete in < 2:45 @21:56:37.02 PCR.run 10:00 @21:59:32.79 PCR.off @21:59:32.83 AFV.select :carrierSolution @21:59:32.95 delay 2 seconds @21:59:34.97 AS.seek 1ml @22:00:32.81 delay 1 second @22:00:33.83 AFV.select :holdingCoil @22:00:33.94 delay 1 second @22:00:34.96 AS.reconfigure ASfast @22:00:35.01 AS.seek 0ml @22:01:02.38 delay 3 seconds @22:01:05.40 AFV.select :carrierSolution @22:01:05.51 delay 2 seconds @22:01:07.53 AS.reconfigure ASconfig @22:01:07.59 AS.seek 1ml @22:02:05.36 delay 1 second @22:02:06.38 AFV.select :holdingCoil @22:02:06.49 delay 1 second @22:02:07.51 AS.reconfigure ASfast @22:02:07.56 AS.seek 0ml @22:02:34.94 delay 3 seconds @22:02:37.96 AFV.select :carrierSolution @22:02:38.07 delay 2 seconds @22:02:40.09 AS.reconfigure ASconfig @22:02:40.15 AS.seek 1ml @22:03:37.91 delay 1 second @22:03:38.93 AFV.select :holdingCoil @22:03:39.04 delay 1 second @22:03:40.06 AS.reconfigure ASfast @22:03:40.11 AS.seek 0ml @22:04:07.48 delay 3 seconds @22:04:10.50 AFV.select :carrierSolution @22:04:10.61 delay 2 seconds @22:04:12.63 AS.reconfigure ASconfig @22:04:12.69 AS.seek 1ml @22:05:10.48 delay 1 second @22:05:11.50 AFV.select :holdingCoil @22:05:11.61 delay 1 second @22:05:12.63 AS.reconfigure ASfast @22:05:12.68 AS.seek 0ml @22:05:40.06 delay 3 seconds @22:05:43.08 AFV.select :carrierSolution @22:05:43.19 delay 2 seconds @22:05:45.21 AS.reconfigure ASconfig @22:05:45.27 AS.seek 1ml @22:06:43.08 delay 1 second @22:06:44.10 AFV.select :holdingCoil @22:06:44.21 delay 1 second @22:06:45.23 AS.reconfigure ASnorm @22:06:45.28 AS.seek 0ml @22:07:36.02
AFV.select :carrierSolution @22:07:36.13 delay 2 seconds @22:07:38.15 AS.reconfigure ASconfig @22:07:38.20 AS.seek 0.5ml @22:08:07.28 delay 1 second @22:08:08.30 AFV.select :holdingCoil @22:08:08.41 ATV.dial :air1 @22:08:09.92 AS.reconfigure ASslow2 @22:08:09.98 AS.seek 0.506ml @22:08:16.68 ATV.dial :extSrc @22:08:18.40 AS.seek 0.512ml @22:08:24.89 delay 5 seconds @22:08:29.92 ATV.dial :air1 @22:08:31.64 AS.reconfigure ASslow1 @22:08:31.70 AS.seek 0.542ml @22:08:37.97 ATV.dial :waste @22:08:38.54 AS.reconfigure ASconfig @22:08:38.59 AS.seek :empty @22:09:10.25 Setting up PCR for Pp -- writing /var/log/esp/Pp19may0822h.pcr @22:09:10.27 Building PCR Slug from extSrc with MM1 mix, PP2 primer @22:09:10.28 Priming with carrier @22:09:10.31 ATV.dial ARV @22:09:11.83 ARV.dial :carrier @22:09:12.20 AS.reconfigure ASnorm @22:09:12.27 AS.seek 0.499ml @22:09:37.84 AFV.select :carrierSolution @22:09:37.97 delay 2 seconds @22:09:39.99 AS.reconfigure ASconfig @22:09:40.06 AS.seek 1ml @22:10:09.14 delay 1 second @22:10:10.16 AFV.select :holdingCoil @22:10:10.27 delay 1 second @22:10:11.28 ATV.dial :waste @22:10:12.98 AS.reconfigure ASnorm @22:10:13.04 AS.seek :empty @22:11:03.80 AFV.select :carrierSolution @22:11:03.92 delay 2 seconds @22:11:05.94 AS.reconfigure ASconfig @22:11:06.01 AS.seek 0.5ml @22:11:35.13 delay 1 second @22:11:36.15 AFV.select :holdingCoil @22:11:36.26 Building Slug @22:11:36.27 ATV.dial :ARV @22:11:37.95 delay 2 seconds @22:11:39.97 ARV.dial :air1 @22:11:40.38 delay 2 seconds @22:11:42.46 AS.reconfigure ASslow2 @22:11:42.52 AS.seek 0.506ml @22:11:48.73 delay 2 seconds @22:11:50.75 ARV.dial :MM1 @22:11:51.36 delay 2 seconds @22:11:53.39 Adding MM1 @22:11:53.46 AS.seek 0.524ml @22:12:11.78 delay 25 seconds @22:12:36.80 ARV.dial :PP2 @22:12:37.19 Adding PP2 @22:12:37.21 delay 2 seconds @22:12:39.29 AS.seek 0.53ml @22:12:45.67 delay 10 seconds @22:12:55.70 ARV.dial :air2 @22:12:56.09 adding ARV air @22:12:56.11 delay 2 seconds @22:12:58.19 AS.seek 0.536ml @22:13:04.41 delay 5 seconds @22:13:09.43 ARV.dial :carrier @22:13:10.70 delay 7 seconds @22:13:17.79 AS.seek 0.594ml @22:14:16.39 delay 5 seconds @22:14:21.41 ATV.dial :extSrc @22:14:21.99 delay 2 seconds @22:14:24.07 AS.seek 0.6ml @22:14:30.42 delay 4 seconds @22:14:34.44 ATV.dial ARV @22:14:35.00 delay 2 seconds @22:14:37.02 ARV.dial :carrier @22:14:37.04 delay 2 seconds @22:14:39.12 AS.seek 0.65ml @22:15:29.56 delay 4 seconds @22:15:33.58 ATV.dial PCR @22:15:34.36 ASV.dial :waste1 @22:15:34.37 ARV.dial :air1 @22:15:34.80 Positioning Slug by 0.324ml @22:15:34.88 AS.seek 0.326ml @22:21:01.02 delay 5 seconds @22:21:06.04 ATV.dial ARV @22:21:06.83 ASV.dial :PCRlock @22:21:07.24 delay 5 seconds @22:21:12.27 Running Pp Profile60C350 thermocycling @22:21:12.28 PCR.program Profile60C350 @22:21:12.43 Profile60C350 should complete in < 1:48:05 @22:21:12.44 PCR.run 2:20:06.25 @23:44:37.34 PCR.off @23:44:39.33 PCR for Pp completed -- closed /var/log/esp/Pp19may0822h.pcr @23:44:39.41 Cleaning PCR @23:44:39.42 AFV.select :carrierSolution @23:44:39.54 delay 2 seconds @23:44:41.56 AS.reconfigure ASconfig @23:44:41.61 AS.seek 0.5ml @23:44:51.92 delay 1 second @23:44:52.94 AFV.select :holdingCoil @23:44:53.04 ASV.dial :waste1 @23:44:53.44 ATV.dial :air3 @23:44:54.77 AS.reconfigure ASslow1 @23:44:54.83 AS.seek 0.51ml @23:44:57.14 delay 2 seconds @23:44:59.16 ATV.dial :bleach @23:44:59.80 AS.seek 0.81ml @23:46:00.41 delay 3 seconds @23:46:03.43 ATV.dial :air3 @23:46:03.99 delay 3 seconds @23:46:07.06 AS.seek 0.835ml @23:46:12.37 delay 3 seconds @23:46:15.39 ATV.dial :bleach @23:46:16.02 AS.seek 0.82ml @23:46:19.31 delay 3 seconds @23:46:22.33 ARV.dial :waste @23:46:22.96 delay 3 seconds @23:46:25.98 ATV.dial :ARV @23:46:26.91 AS.reconfigure ASconfig @23:46:26.96 AS.seek 0.47ml @23:46:47.39 AFV.select :carrierSolution @23:46:47.51 delay 2 seconds @23:46:49.53 AS.seek 1ml @23:47:20.30 delay 1 second @23:47:21.32 AFV.select :holdingCoil @23:47:21.75 delay 1 second @23:47:22.83 AS.reconfigure ASfast @23:47:22.89 AS.seek 0ml @23:47:50.25 AFV.select :carrierSolution @23:47:50.37 delay 2 seconds @23:47:52.39 AS.reconfigure ASconfig @23:47:52.45 AS.seek 1ml @23:48:50.19 delay 1 second @23:48:51.21 AFV.select :holdingCoil @23:48:51.32 delay 1 second @23:48:52.34 AS.reconfigure ASfast @23:48:52.42 AS.seek 0ml @23:49:19.80 AFV.select :carrierSolution @23:49:19.92 delay 2 seconds @23:49:21.94 AS.reconfigure ASconfig @23:49:22.00 AS.seek 1ml @23:50:19.77 delay 1 second @23:50:20.79 AFV.select :holdingCoil @23:50:20.90 delay 1 second @23:50:21.92 AS.reconfigure ASfast @23:50:21.98 AS.seek 0ml @23:50:49.33 ARV.dial between carrier and air1 @23:50:49.94 ATV.dial :air3 @23:50:51.25 AS.reconfigure ASslow1 @23:50:51.31 AS.seek 0.01ml @23:50:53.64 delay 2 seconds @23:50:55.66 ATV.dial :bleach @23:50:56.30 AS.seek 0.81ml @23:53:37.44 delay 3 seconds @23:53:40.46 ATV.dial :air3 @23:53:41.03 delay 3 seconds @23:53:44.11 AS.seek 0.835ml @23:53:49.41 delay 3 seconds @23:53:52.43 ATV.dial :bleach @23:53:53.06 AS.seek 0.82ml @23:53:56.39 delay 3 seconds @23:53:59.41 ATV.dial PCR @23:53:59.84 AS.reconfigure ASconfig @23:53:59.89 AS.seek :empty @23:54:47.36 PCR.program :bleachProfile @23:54:47.42 PCR program should complete in < 2:45 @23:54:47.43 PCR.run 10:00 @23:57:43.21 PCR.off @23:57:43.25 AFV.select :carrierSolution @23:57:43.37 delay 2 seconds @23:57:45.39 AS.seek 1ml @23:58:43.22 delay 1 second @23:58:44.24 AFV.select :holdingCoil @23:58:44.35 delay 1 second @23:58:45.37 AS.reconfigure ASfast @23:58:45.43 AS.seek 0ml @23:59:12.80 delay 3 seconds @23:59:15.82 AFV.select :carrierSolution @23:59:15.93 delay 2 seconds @23:59:17.95 AS.reconfigure ASconfig @23:59:18.01 AS.seek 1ml @00:00:15.75CEST09-May-19 delay 1 second @00:00:16.77 AFV.select :holdingCoil @00:00:16.88 delay 1 second @00:00:17.90 AS.reconfigure ASfast @00:00:17.95 AS.seek 0ml @00:00:45.33 delay 3 seconds @00:00:48.35 AFV.select :carrierSolution @00:00:48.46 delay 2 seconds @00:00:50.48 AS.reconfigure ASconfig @00:00:50.54 AS.seek 1ml @00:01:48.30 delay 1 second @00:01:49.32 AFV.select :holdingCoil @00:01:49.43 delay 1 second @00:01:50.45 AS.reconfigure ASfast @00:01:50.50 AS.seek 0ml @00:02:17.86 delay 3 seconds @00:02:20.88 AFV.select :carrierSolution @00:02:20.99 delay 2 seconds @00:02:23.01 AS.reconfigure ASconfig @00:02:23.09 AS.seek 1ml @00:03:20.83 delay 1 second @00:03:21.85 AFV.select :holdingCoil @00:03:21.96 delay 1 second @00:03:22.98 AS.reconfigure ASfast @00:03:23.03 AS.seek 0ml @00:03:50.41 delay 3 seconds @00:03:53.43 AFV.select :carrierSolution @00:03:53.54 delay 2 seconds @00:03:55.56 AS.reconfigure ASconfig @00:03:55.62 AS.seek 1ml @00:04:53.39 delay 1 second @00:04:54.41 AFV.select :holdingCoil @00:04:54.52 delay 1 second @00:04:55.54 AS.reconfigure ASnorm @00:04:55.59 AS.seek 0ml @00:05:46.32
AFV.select :carrierSolution @00:05:46.43 delay 2 seconds @00:05:48.45 AS.reconfigure ASconfig @00:05:48.50 AS.seek 0.5ml @00:06:17.56 delay 1 second @00:06:18.58 AFV.select :holdingCoil @00:06:18.70 ATV.dial :air1 @00:06:20.22 AS.reconfigure ASslow1 @00:06:20.27 AS.seek 0.52ml @00:06:24.58 delay 5 seconds @00:06:29.60 ATV.dial :extSrc @00:06:31.34 AS.reconfigure ASslow2 @00:06:31.40 AS.seek 0.545ml @00:06:56.92 delay 10 seconds @00:07:06.94 ATV.dial :air1 @00:07:09.03 AS.reconfigure ASslow1 @00:07:09.09 AS.seek 0.575ml @00:07:15.41 ATV.dial :waste @00:07:15.98 AS.reconfigure ASconfig @00:07:16.03 AS.seek :empty @00:07:49.59 Setting up PCR for Pp -- writing /var/log/esp/Pp19may0900h.pcr @00:07:49.62 Building PCR Slug from extSrc with MM1 mix, PP2 primer @00:07:49.65 Priming with carrier @00:07:49.66 ATV.dial ARV @00:07:51.19 ARV.dial :carrier @00:07:51.53 AS.reconfigure ASnorm @00:07:51.60 AS.seek 0.499ml @00:08:17.17 AFV.select :carrierSolution @00:08:17.29 delay 2 seconds @00:08:19.31 AS.reconfigure ASconfig @00:08:19.38 AS.seek 1ml @00:08:48.44 delay 1 second @00:08:49.46 AFV.select :holdingCoil @00:08:49.57 delay 1 second @00:08:50.58 ATV.dial :waste @00:08:52.30 AS.reconfigure ASnorm @00:08:52.36 AS.seek :empty @00:09:43.11 AFV.select :carrierSolution @00:09:43.24 delay 2 seconds @00:09:45.26 AS.reconfigure ASconfig @00:09:45.33 AS.seek 0.5ml @00:10:14.44 delay 1 second @00:10:15.46 AFV.select :holdingCoil @00:10:15.57 Building Slug @00:10:15.58 ATV.dial :ARV @00:10:17.26 delay 2 seconds @00:10:19.28 ARV.dial :air1 @00:10:19.79 delay 2 seconds @00:10:21.86 AS.reconfigure ASslow2 @00:10:21.93 AS.seek 0.506ml @00:10:28.38 delay 2 seconds @00:10:30.40 ARV.dial :MM1 @00:10:31.00 delay 2 seconds @00:10:33.03 Adding MM1 @00:10:33.10 AS.seek 0.524ml @00:10:51.53 delay 25 seconds @00:11:16.55 ARV.dial :PP2 @00:11:16.96 Adding PP2 @00:11:16.98 delay 2 seconds @00:11:19.06 AS.seek 0.53ml @00:11:25.42 delay 10 seconds @00:11:35.45 ARV.dial :air2 @00:11:35.85 adding ARV air @00:11:35.87 delay 2 seconds @00:11:37.95 AS.seek 0.536ml @00:11:44.27 delay 5 seconds @00:11:49.29 ARV.dial :carrier @00:11:50.56 delay 7 seconds @00:11:57.65 AS.seek 0.594ml @00:12:56.42 delay 5 seconds @00:13:01.44 ATV.dial :extSrc @00:13:02.02 delay 2 seconds @00:13:04.10 AS.seek 0.6ml @00:13:10.30 delay 4 seconds @00:13:14.32 ATV.dial ARV @00:13:14.88 delay 2 seconds @00:13:16.90 ARV.dial :carrier @00:13:16.92 delay 2 seconds @00:13:19.00 AS.seek 0.65ml @00:14:09.52 delay 4 seconds @00:14:13.54 ATV.dial PCR @00:14:14.31 ASV.dial :waste1 @00:14:14.32 ARV.dial :air1 @00:14:14.75 Positioning Slug by 0.324ml @00:14:14.85 AS.seek 0.326ml @00:19:40.97 delay 5 seconds @00:19:45.99 ATV.dial ARV @00:19:46.78 ASV.dial :PCRlock @00:19:47.17 delay 5 seconds @00:19:52.20 Running Pp Profile60C350 thermocycling @00:19:52.21 PCR.program Profile60C350 @00:19:52.36 Profile60C350 should complete in < 1:48:05 @00:19:52.37 PCR.run 2:20:06.25 @01:43:23.58 PCR.off @01:43:25.56 PCR for Pp completed -- closed /var/log/esp/Pp19may0900h.pcr @01:43:25.64 Cleaning PCR @01:43:25.65 AFV.select :carrierSolution @01:43:25.78 delay 2 seconds @01:43:27.80 AS.reconfigure ASconfig @01:43:27.86 AS.seek 0.5ml @01:43:38.18 delay 1 second @01:43:39.20 AFV.select :holdingCoil @01:43:39.30 ASV.dial :waste1 @01:43:39.71 ATV.dial :air3 @01:43:41.03 AS.reconfigure ASslow1 @01:43:41.09 AS.seek 0.51ml @01:43:43.38 delay 2 seconds @01:43:45.40 ATV.dial :bleach @01:43:46.03 AS.seek 0.81ml @01:44:46.64 delay 3 seconds @01:44:49.66 ATV.dial :air3 @01:44:50.22 delay 3 seconds @01:44:53.30 AS.seek 0.835ml @01:44:58.60 delay 3 seconds @01:45:01.62 ATV.dial :bleach @01:45:02.24 AS.seek 0.82ml @01:45:05.55 delay 3 seconds @01:45:08.57 ARV.dial :waste @01:45:09.19 delay 3 seconds @01:45:12.21 ATV.dial :ARV @01:45:13.13 AS.reconfigure ASconfig @01:45:13.19 AS.seek 0.47ml @01:45:33.60 AFV.select :carrierSolution @01:45:33.72 delay 2 seconds @01:45:35.74 AS.seek 1ml @01:46:06.53 delay 1 second @01:46:07.55 AFV.select :holdingCoil @01:46:07.66 delay 1 second @01:46:08.68 AS.reconfigure ASfast @01:46:08.74 AS.seek 0ml @01:46:36.10 AFV.select :carrierSolution @01:46:36.22 delay 2 seconds @01:46:38.24 AS.reconfigure ASconfig @01:46:38.30 AS.seek 1ml @01:47:36.43 delay 1 second @01:47:37.45 AFV.select :holdingCoil @01:47:37.56 delay 1 second @01:47:38.58 AS.reconfigure ASfast @01:47:38.64 AS.seek 0ml @01:48:06.05 AFV.select :carrierSolution @01:48:06.18 delay 2 seconds @01:48:08.20 AS.reconfigure ASconfig @01:48:08.25 AS.seek 1ml @01:49:06.05 delay 1 second @01:49:07.07 AFV.select :holdingCoil @01:49:07.18 delay 1 second @01:49:08.20 AS.reconfigure ASfast @01:49:08.26 AS.seek 0ml @01:49:35.61 ARV.dial between carrier and air1 @01:49:36.11 ATV.dial :air3 @01:49:37.44 AS.reconfigure ASslow1 @01:49:37.50 AS.seek 0.01ml @01:49:39.83 delay 2 seconds @01:49:41.85 ATV.dial :bleach @01:49:42.49 AS.seek 0.81ml @01:52:23.63 delay 3 seconds @01:52:26.65 ATV.dial :air3 @01:52:27.21 delay 3 seconds @01:52:30.29 AS.seek 0.835ml @01:52:35.60 delay 3 seconds @01:52:38.62 ATV.dial :bleach @01:52:39.25 AS.seek 0.82ml @01:52:42.58 delay 3 seconds @01:52:45.60 ATV.dial PCR @01:52:46.03 AS.reconfigure ASconfig @01:52:46.08 AS.seek :empty @01:53:33.57 PCR.program :bleachProfile @01:53:33.63 PCR program should complete in < 2:45 @01:53:33.64 PCR.run 10:00 @01:56:29.41 PCR.off @01:56:29.45 AFV.select :carrierSolution @01:56:29.57 delay 2 seconds @01:56:31.59 AS.seek 1ml @01:57:29.43 delay 1 second @01:57:30.45 AFV.select :holdingCoil @01:57:30.56 delay 1 second @01:57:31.58 AS.reconfigure ASfast @01:57:31.64 AS.seek 0ml @01:57:58.99 delay 3 seconds @01:58:02.01 AFV.select :carrierSolution @01:58:02.12 delay 2 seconds @01:58:04.14 AS.reconfigure ASconfig @01:58:04.20 AS.seek 1ml @01:59:01.99 delay 1 second @01:59:03.01 AFV.select :holdingCoil @01:59:03.12 delay 1 second @01:59:04.14 AS.reconfigure ASfast @01:59:04.20 AS.seek 0ml @01:59:31.57 delay 3 seconds @01:59:34.59 AFV.select :carrierSolution @01:59:34.70 delay 2 seconds @01:59:36.72 AS.reconfigure ASconfig @01:59:36.78 AS.seek 1ml @02:00:34.54 delay 1 second @02:00:35.56 AFV.select :holdingCoil @02:00:35.67 delay 1 second @02:00:36.69 AS.reconfigure ASfast @02:00:36.75 AS.seek 0ml @02:01:04.10 delay 3 seconds @02:01:07.12 AFV.select :carrierSolution @02:01:07.23 delay 2 seconds @02:01:09.25 AS.reconfigure ASconfig @02:01:09.31 AS.seek 1ml @02:02:07.11 delay 1 second @02:02:08.13 AFV.select :holdingCoil @02:02:08.24 delay 1 second @02:02:09.26 AS.reconfigure ASfast @02:02:09.32 AS.seek 0ml @02:02:36.68 delay 3 seconds @02:02:39.70 AFV.select :carrierSolution @02:02:39.81 delay 2 seconds @02:02:41.83 AS.reconfigure ASconfig @02:02:41.90 AS.seek 1ml @02:03:39.69 delay 1 second @02:03:40.71 AFV.select :holdingCoil @02:03:40.82 delay 1 second @02:03:41.84 AS.reconfigure ASnorm @02:03:41.90 AS.seek 0ml @02:04:32.63
AFV.select :carrierSolution @02:04:32.74 delay 2 seconds @02:04:34.76 AS.reconfigure ASconfig @02:04:34.82 AS.seek 0.5ml @02:05:03.88 delay 1 second @02:05:04.90 AFV.select :holdingCoil @02:05:05.02 ATV.dial :air1 @02:05:06.52 AS.reconfigure ASslow2 @02:05:06.58 AS.seek 0.506ml @02:05:13.21 ATV.dial :extSrc @02:05:14.94 AS.seek 0.512ml @02:05:21.35 delay 5 seconds @02:05:26.37 ATV.dial :air1 @02:05:28.11 AS.reconfigure ASslow1 @02:05:28.16 AS.seek 0.542ml @02:05:34.49 ATV.dial :waste @02:05:35.05 AS.reconfigure ASconfig @02:05:35.11 AS.seek :empty @02:06:07.15 Setting up PCR for Pp -- writing /var/log/esp/Pp19may0902h.pcr @02:06:07.17 Building PCR Slug from extSrc with MM1 mix, PP2 primer @02:06:07.20 Priming with carrier @02:06:07.23 ATV.dial ARV @02:06:08.75 ARV.dial :carrier @02:06:09.10 AS.reconfigure ASnorm @02:06:09.17 AS.seek 0.499ml @02:06:34.75 AFV.select :carrierSolution @02:06:34.88 delay 2 seconds @02:06:36.90 AS.reconfigure ASconfig @02:06:36.97 AS.seek 1ml @02:07:06.05 delay 1 second @02:07:07.07 AFV.select :holdingCoil @02:07:07.18 delay 1 second @02:07:08.19 ATV.dial :waste @02:07:09.90 AS.reconfigure ASnorm @02:07:09.96 AS.seek :empty @02:08:00.69 AFV.select :carrierSolution @02:08:00.82 delay 2 seconds @02:08:02.84 AS.reconfigure ASconfig @02:08:02.91 AS.seek 0.5ml @02:08:31.99 delay 1 second @02:08:33.01 AFV.select :holdingCoil @02:08:33.12 Building Slug @02:08:33.13 ATV.dial :ARV @02:08:34.80 delay 2 seconds @02:08:36.82 ARV.dial :air1 @02:08:37.23 delay 2 seconds @02:08:39.30 AS.reconfigure ASslow2 @02:08:39.37 AS.seek 0.506ml @02:08:45.68 delay 2 seconds @02:08:47.70 ARV.dial :MM1 @02:08:48.39 delay 2 seconds @02:08:50.42 Adding MM1 @02:08:50.49 AS.seek 0.524ml @02:09:09.00 delay 25 seconds @02:09:34.02 ARV.dial :PP2 @02:09:34.43 Adding PP2 @02:09:34.45 delay 2 seconds @02:09:36.53 AS.seek 0.53ml @02:09:42.74 delay 10 seconds @02:09:52.77 ARV.dial :air2 @02:09:53.16 adding ARV air @02:09:53.18 delay 2 seconds @02:09:55.26 AS.seek 0.536ml @02:10:01.71 delay 5 seconds @02:10:06.73 ARV.dial :carrier @02:10:08.00 delay 7 seconds @02:10:15.09 AS.seek 0.594ml @02:11:13.68 delay 5 seconds @02:11:18.70 ATV.dial :extSrc @02:11:19.27 delay 2 seconds @02:11:21.35 AS.seek 0.6ml @02:11:27.58 delay 4 seconds @02:11:31.60 ATV.dial ARV @02:11:32.16 delay 2 seconds @02:11:34.18 ARV.dial :carrier @02:11:34.20 delay 2 seconds @02:11:36.28 AS.seek 0.65ml @02:12:26.77 delay 4 seconds @02:12:30.79 ATV.dial PCR @02:12:31.57 ASV.dial :waste1 @02:12:31.58 ARV.dial :air1 @02:12:32.02 Positioning Slug by 0.324ml @02:12:32.10 AS.seek 0.326ml @02:17:58.33 delay 5 seconds @02:18:03.35 ATV.dial ARV @02:18:04.18 ASV.dial :PCRlock @02:18:04.58 delay 5 seconds @02:18:09.61 Running Pp Profile60C350 thermocycling @02:18:09.62 PCR.program Profile60C350 @02:18:09.77 Profile60C350 should complete in < 1:48:05 @02:18:09.78 PCR.run 2:20:06.25 @03:23:16.26 Session Ended @03:41:30.92 PCR.off @03:41:32.90 PCR for Pp completed -- closed /var/log/esp/Pp19may0902h.pcr @03:41:32.96 Cleaning PCR @03:41:32.97 AFV.select :carrierSolution @03:41:33.07 delay 2 seconds @03:41:35.09 AS.reconfigure ASconfig @03:41:35.14 AS.seek 0.5ml @03:41:45.40 delay 1 second @03:41:46.42 AFV.select :holdingCoil @03:41:46.52 ASV.dial :waste1 @03:41:46.91 ATV.dial :air3 @03:41:48.21 AS.reconfigure ASslow1 @03:41:48.25 AS.seek 0.51ml @03:41:50.53 delay 2 seconds @03:41:52.55 ATV.dial :bleach @03:41:53.16 AS.seek 0.81ml @03:42:53.74 delay 3 seconds @03:42:56.76 ATV.dial :air3 @03:42:57.30 delay 3 seconds @03:43:00.38 AS.seek 0.835ml @03:43:05.66 delay 3 seconds @03:43:08.68 ATV.dial :bleach @03:43:09.28 AS.seek 0.82ml @03:43:12.57 delay 3 seconds @03:43:15.59 ARV.dial :waste @03:43:16.19 delay 3 seconds @03:43:19.21 ATV.dial :ARV @03:43:20.11 AS.reconfigure ASconfig @03:43:20.15 AS.seek 0.47ml @03:43:40.55 AFV.select :carrierSolution @03:43:40.66 delay 2 seconds @03:43:42.68 AS.seek 1ml @03:44:13.46 delay 1 second @03:44:14.48 AFV.select :holdingCoil @03:44:14.59 delay 1 second @03:44:15.61 AS.reconfigure ASfast @03:44:15.66 AS.seek 0ml @03:44:43.01 AFV.select :carrierSolution @03:44:43.12 delay 2 seconds @03:44:45.14 AS.reconfigure ASconfig @03:44:45.19 AS.seek 1ml @03:45:42.96 delay 1 second @03:45:43.98 AFV.select :holdingCoil @03:45:44.09 delay 1 second @03:45:45.11 AS.reconfigure ASfast @03:45:45.16 AS.seek 0ml @03:46:12.49 AFV.select :carrierSolution @03:46:12.60 delay 2 seconds @03:46:14.93 AS.reconfigure ASconfig @03:46:15.03 AS.seek 1ml @03:47:12.82 delay 1 second @03:47:13.84 AFV.select :holdingCoil @03:47:13.95 delay 1 second @03:47:14.97 AS.reconfigure ASfast @03:47:15.02 AS.seek 0ml @03:47:42.44 ARV.dial between carrier and air1 @03:47:42.93 ATV.dial :air3 @03:47:44.22 AS.reconfigure ASslow1 @03:47:44.26 AS.seek 0.01ml @03:47:46.58 delay 2 seconds @03:47:48.60 ATV.dial :bleach @03:47:49.21 AS.seek 0.81ml @03:50:30.33 delay 3 seconds @03:50:33.35 ATV.dial :air3 @03:50:33.90 delay 3 seconds @03:50:36.98 AS.seek 0.835ml @03:50:42.27 delay 3 seconds @03:50:45.29 ATV.dial :bleach @03:50:45.89 AS.seek 0.82ml @03:50:49.19 delay 3 seconds @03:50:52.21 ATV.dial PCR @03:50:52.63 AS.reconfigure ASconfig @03:50:52.67 AS.seek :empty @03:51:40.13 PCR.program :bleachProfile @03:51:40.18 PCR program should complete in < 2:45 @03:51:40.19 PCR.run 10:00 @03:54:35.95 PCR.off @03:54:35.98 AFV.select :carrierSolution @03:54:36.08 delay 2 seconds @03:54:38.10 AS.seek 1ml @03:55:35.94 delay 1 second @03:55:36.96 AFV.select :holdingCoil @03:55:37.07 delay 1 second @03:55:38.09 AS.reconfigure ASfast @03:55:38.14 AS.seek 0ml @03:56:05.47 delay 3 seconds @03:56:08.49 AFV.select :carrierSolution @03:56:08.60 delay 2 seconds @03:56:10.62 AS.reconfigure ASconfig @03:56:10.68 AS.seek 1ml @03:57:08.41 delay 1 second @03:57:09.43 AFV.select :holdingCoil @03:57:09.54 delay 1 second @03:57:10.56 AS.reconfigure ASfast @03:57:10.61 AS.seek 0ml @03:57:37.93 delay 3 seconds @03:57:40.95 AFV.select :carrierSolution @03:57:41.06 delay 2 seconds @03:57:43.08 AS.reconfigure ASconfig @03:57:43.13 AS.seek 1ml @03:58:40.91 delay 1 second @03:58:41.93 AFV.select :holdingCoil @03:58:42.04 delay 1 second @03:58:43.06 AS.reconfigure ASfast @03:58:43.11 AS.seek 0ml @03:59:10.46 delay 3 seconds @03:59:13.48 AFV.select :carrierSolution @03:59:13.59 delay 2 seconds @03:59:15.61 AS.reconfigure ASconfig @03:59:15.67 AS.seek 1ml @04:00:13.40 delay 1 second @04:00:14.42 AFV.select :holdingCoil @04:00:14.53 delay 1 second @04:00:15.55 AS.reconfigure ASfast @04:00:15.60 AS.seek 0ml @04:00:42.93 delay 3 seconds @04:00:45.95 AFV.select :carrierSolution @04:00:46.06 delay 2 seconds @04:00:48.08 AS.reconfigure ASconfig @04:00:48.13 AS.seek 1ml @04:01:45.88 delay 1 second @04:01:46.90 AFV.select :holdingCoil @04:01:47.01 delay 1 second @04:01:48.03 AS.reconfigure ASnorm @04:01:48.08 AS.seek 0ml @04:02:38.81
AFV.select :carrierSolution @04:02:38.91 delay 2 seconds @04:02:40.93 AS.reconfigure ASconfig @04:02:40.98 AS.seek 0.5ml @04:03:10.01 delay 1 second @04:03:11.03 AFV.select :holdingCoil @04:03:11.13 ATV.dial :air1 @04:03:12.63 AS.reconfigure ASslow1 @04:03:12.67 AS.seek 0.52ml @04:03:16.96 delay 5 seconds @04:03:21.98 ATV.dial :extSrc @04:03:23.71 AS.reconfigure ASslow2 @04:03:23.75 AS.seek 0.538ml @04:03:41.99 delay 10 seconds @04:03:52.01 ATV.dial :air1 @04:03:53.72 AS.reconfigure ASslow1 @04:03:53.76 AS.seek 0.568ml @04:04:00.05 ATV.dial :waste @04:04:00.61 AS.reconfigure ASconfig @04:04:00.64 AS.seek :empty @04:04:33.66 deconEluateRecoveryLine extSrc @04:04:33.67 ATV.dial :waste @04:04:33.69 AS.reconfigure ASnorm @04:04:33.73 AS.seek 0ml @04:04:34.07 ATV.dial :air3 @04:04:34.75 AS.reconfigure ASslow1 @04:04:35.16 AS.seek 0.01ml @04:04:37.44 delay 2 seconds @04:04:39.46 ATV.dial :bleach @04:04:39.91 AS.seek 0.31ml @04:05:40.58 delay 3 seconds @04:05:43.60 ATV.dial :air3 @04:05:44.15 delay 3 seconds @04:05:47.23 AS.seek 0.335ml @04:05:52.57 delay 3 seconds @04:05:55.59 ATV.dial :bleach @04:05:56.19 AS.seek 0.32ml @04:05:59.46 delay 3 seconds @04:06:02.48 ATV.dial :extSrc @04:06:03.11 AS.seek 0ml @04:07:07.71 AFV.select :carrierSolution @04:07:07.82 delay 2 seconds @04:07:09.84 AS.reconfigure ASconfig @04:07:09.89 AS.seek 0.5ml @04:07:38.90 delay 1 second @04:07:39.92 AFV.select :holdingCoil @04:07:40.02 ATV.dial :extSrc @04:07:40.04 AS.reconfigure ASslow1 @04:07:40.09 AS.seek 0ml @04:09:20.88 AFV.select :carrierSolution @04:09:20.99 delay 2 seconds @04:09:23.01 AS.reconfigure ASconfig @04:09:23.06 AS.seek 0.5ml @04:09:52.10 delay 1 second @04:09:53.12 AFV.select :holdingCoil @04:09:53.22 ATV.dial :extSrc @04:09:53.24 AS.reconfigure ASslow1 @04:09:53.29 AS.seek 0ml @04:11:34.10 AFV.select :carrierSolution @04:11:34.21 delay 2 seconds @04:11:36.23 AS.reconfigure ASconfig @04:11:36.28 AS.seek 0.5ml @04:12:05.30 delay 1 second @04:12:06.32 AFV.select :holdingCoil @04:12:06.42 ATV.dial :extSrc @04:12:06.44 AS.reconfigure ASslow1 @04:12:06.49 AS.seek 0ml @04:13:47.30 AFV.select :carrierSolution @04:13:47.41 delay 2 seconds @04:13:49.43 AS.reconfigure ASconfig @04:13:49.48 AS.seek 0.5ml @04:14:18.51 delay 1 second @04:14:19.53 AFV.select :holdingCoil @04:14:19.63 ATV.dial :extSrc @04:14:19.65 AS.reconfigure ASslow1 @04:14:19.70 AS.seek 0ml @04:16:00.49 ATV.dial :air2 @04:16:01.83 AS.reconfigure ASslow0 @04:16:01.87 AS.seek 0.5ml @04:16:52.47 ATV.dial :extSrc @04:16:53.97 AS.seek 0.02ml @04:17:42.55 delay 1 second @04:17:43.57 ATV.dial :waste @04:17:44.88 AS.reconfigure ASconfig @04:17:44.92 AS.seek :empty @04:17:46.41 deconEluateRecoveryLine extSrc @04:17:46.42 ATV.dial :waste @04:17:46.44 AS.reconfigure ASnorm @04:17:46.48 AS.seek 0ml @04:17:46.82 ATV.dial :air3 @04:17:47.67 AS.reconfigure ASslow1 @04:17:47.71 AS.seek 0.01ml @04:17:49.99 delay 2 seconds @04:17:52.01 ATV.dial :bleach @04:17:52.46 AS.seek 0.31ml @04:18:53.09 delay 3 seconds @04:18:56.10 ATV.dial :air3 @04:18:56.65 delay 3 seconds @04:18:59.72 AS.seek 0.335ml @04:19:05.03 delay 3 seconds @04:19:08.05 ATV.dial :bleach @04:19:08.66 AS.seek 0.32ml @04:19:11.96 delay 3 seconds @04:19:14.98 ATV.dial :extSrc @04:19:15.61 AS.seek 0ml @04:20:20.18 AFV.select :carrierSolution @04:20:20.29 delay 2 seconds @04:20:22.31 AS.reconfigure ASconfig @04:20:22.36 AS.seek 0.5ml @04:20:51.38 delay 1 second @04:20:52.40 AFV.select :holdingCoil @04:20:52.50 ATV.dial :extSrc @04:20:52.52 AS.reconfigure ASslow1 @04:20:52.57 AS.seek 0ml @04:22:33.38 AFV.select :carrierSolution @04:22:33.49 delay 2 seconds @04:22:35.51 AS.reconfigure ASconfig @04:22:35.56 AS.seek 0.5ml @04:23:04.58 delay 1 second @04:23:05.60 AFV.select :holdingCoil @04:23:05.70 ATV.dial :extSrc @04:23:05.72 AS.reconfigure ASslow1 @04:23:05.77 AS.seek 0ml @04:24:46.55 AFV.select :carrierSolution @04:24:46.66 delay 2 seconds @04:24:48.68 AS.reconfigure ASconfig @04:24:48.73 AS.seek 0.5ml @04:25:17.74 delay 1 second @04:25:18.76 AFV.select :holdingCoil @04:25:18.86 ATV.dial :extSrc @04:25:18.88 AS.reconfigure ASslow1 @04:25:18.93 AS.seek 0ml @04:26:59.73 AFV.select :carrierSolution @04:26:59.84 delay 2 seconds @04:27:01.86 AS.reconfigure ASconfig @04:27:01.91 AS.seek 0.5ml @04:27:30.91 delay 1 second @04:27:31.93 AFV.select :holdingCoil @04:27:32.03 ATV.dial :extSrc @04:27:32.05 AS.reconfigure ASslow1 @04:27:32.10 AS.seek 0ml @04:29:12.91 ATV.dial :air2 @04:29:14.61 AS.reconfigure ASslow0 @04:29:14.65 AS.seek 0.5ml @04:30:05.27 ATV.dial :extSrc @04:30:06.77 AS.seek 0.02ml @04:30:55.33 delay 1 second @04:30:56.36 ATV.dial :waste @04:30:57.67 AS.reconfigure ASconfig @04:30:57.71 AS.seek :empty @04:30:59.19 deconEluateRecoveryLine extSrc @04:30:59.20 ATV.dial :waste @04:30:59.22 AS.reconfigure ASnorm @04:30:59.26 AS.seek 0ml @04:30:59.55 ATV.dial :air3 @04:31:00.40 AS.reconfigure ASslow1 @04:31:00.45 AS.seek 0.01ml @04:31:02.73 delay 2 seconds @04:31:04.75 ATV.dial :bleach @04:31:05.22 AS.seek 0.31ml @04:32:05.80 delay 3 seconds @04:32:08.82 ATV.dial :air3 @04:32:09.38 delay 3 seconds @04:32:12.46 AS.seek 0.335ml @04:32:17.72 delay 3 seconds @04:32:20.74 ATV.dial :bleach @04:32:21.36 AS.seek 0.32ml @04:32:24.66 delay 3 seconds @04:32:27.68 ATV.dial :extSrc @04:32:28.31 AS.seek 0ml @04:33:32.88 AFV.select :carrierSolution @04:33:32.99 delay 2 seconds @04:33:35.01 AS.reconfigure ASconfig @04:33:35.06 AS.seek 0.5ml @04:34:04.08 delay 1 second @04:34:05.10 AFV.select :holdingCoil @04:34:05.20 ATV.dial :extSrc @04:34:05.22 AS.reconfigure ASslow1 @04:34:05.27 AS.seek 0ml @04:35:46.08 AFV.select :carrierSolution @04:35:46.19 delay 2 seconds @04:35:48.21 AS.reconfigure ASconfig @04:35:48.26 AS.seek 0.5ml @04:36:17.29 delay 1 second @04:36:18.31 AFV.select :holdingCoil @04:36:18.41 ATV.dial :extSrc @04:36:18.43 AS.reconfigure ASslow1 @04:36:18.48 AS.seek 0ml @04:37:59.26 AFV.select :carrierSolution @04:37:59.37 delay 2 seconds @04:38:01.39 AS.reconfigure ASconfig @04:38:01.44 AS.seek 0.5ml @04:38:30.46 delay 1 second @04:38:31.48 AFV.select :holdingCoil @04:38:31.58 ATV.dial :extSrc @04:38:31.60 AS.reconfigure ASslow1 @04:38:31.65 AS.seek 0ml @04:40:12.46 AFV.select :carrierSolution @04:40:12.57 delay 2 seconds @04:40:14.59 AS.reconfigure ASconfig @04:40:14.64 AS.seek 0.5ml @04:40:43.66 delay 1 second @04:40:44.68 AFV.select :holdingCoil @04:40:44.78 ATV.dial :extSrc @04:40:44.80 AS.reconfigure ASslow1 @04:40:44.85 AS.seek 0ml @04:42:25.66 ATV.dial :air2 @04:42:27.00 AS.reconfigure ASslow0 @04:42:27.04 AS.seek 0.5ml @04:43:17.57 ATV.dial :extSrc @04:43:19.07 AS.seek 0.02ml @04:44:07.68 delay 1 second @04:44:08.71 ATV.dial :waste @04:44:10.01 AS.reconfigure ASconfig @04:44:10.05 AS.seek :empty @15:26:33.36CEST09-May-19
ESP2 v3.30 I2C Gateway v3.06 for core bus @15:26:35.03 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @15:26:35.28 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @15:26:39.97 Polling Can every 10:00 @15:26:42.14 SPE.coast @15:26:42.18 SPE.configure SPEconfig @15:26:42.26 AS.reconfigure ASconfig @15:26:42.30 AS.hold @15:26:42.35 AS.configure ASconfig @15:26:42.44 SS.configure SSconfig @15:26:42.52 CC.configure CCconfig @15:26:42.60 CS.configure CSconfig @15:26:42.68 PC.configure PCconfig @15:26:42.76 PS.configure PSconfig @15:26:42.85 Forearm.reconfigure LooseForearm @15:26:42.90 Forearm.hold @15:26:42.95 Forearm.configure LooseForearm @15:26:43.41 Elbow.reconfigure LooseElbow @15:26:43.49 Elbow.hold @15:26:43.59 Elbow.configure LooseElbow @15:26:43.79 SC.configure SCloose @15:26:43.95 SE.configure SEconfig @15:26:44.01 CH.coast @15:26:44.11 CH.configure CHconfig @15:26:44.16 PH.coast @15:26:44.21 PH.configure PHconfig @15:26:44.24 PCR.off @15:26:44.27 PCR.pidtemp= 50,0.1,0,300,25,25 @15:26:44.30 PCR.caltemp= 2.8814,0.0372,0 @15:26:44.33 All dwarf controllers running firmware version 3.75 @15:26:44.48 Cleanly exited at 15:24:14CEST09-May-19 @15:26:44.51 Loading /home/esp/esp2/mission/stdMERwithoutESPready2.rb mission @15:26:44.53 AFV.select :carrierSolution @15:26:44.63 delay 2 seconds @15:26:45.96 Session Begun @15:26:46.68
AS.seek 0.5ml @15:27:15.80 delay 1 second @15:27:16.82 AFV.select :holdingCoil @15:27:16.92 ATV.dial :extSrc @15:27:18.43 AS.reconfigure ASslow1 @15:27:18.49 AS.seek 0.53ml @15:27:24.80 delay 5 seconds @15:27:29.88 AS.reconfigure ASslow2 @15:27:29.93 AS.seek 0.54ml @15:27:40.24 delay 20 seconds @15:28:00.31 AS.reconfigure ASslow1 @15:28:00.37 AS.seek 0.57ml @15:28:06.65 delay 5 seconds @15:28:11.73 AS.reconfigure ASslow2 @15:28:11.79 AS.seek 0.58ml @15:28:22.22 delay 20 seconds @15:28:42.29 AS.reconfigure ASslow1 @15:28:42.34 AS.seek 0.61ml @15:28:48.66 delay 5 seconds @15:28:53.74 AS.reconfigure ASslow2 @15:28:53.80 AS.seek 0.62ml @15:29:04.05 delay 20 seconds @15:29:24.12 AS.reconfigure ASslow1 @15:29:24.18 AS.seek 0.65ml @15:29:30.55 delay 5 seconds @15:29:35.63 AS.reconfigure ASslow2 @15:29:35.69 AS.seek 0.66ml @15:29:46.12 delay 20 seconds @15:30:06.15 AS.reconfigure ASslow1 @15:30:06.20 AS.seek 0.705ml @15:30:13.82 -> Abort! @15:30:13.84 NoMethodError in bkha -- undefined method `Abort!' for # @15:30:15.58
delay 10 seconds @15:30:25.60 ATV.dial :air1 @15:30:27.34 AS.seek 0.725ml @15:30:31.57 delay 3 seconds @15:30:34.59 ATV.dial :waste @15:30:35.15 AS.reconfigure ASslow0 @15:30:35.21 AS.seek :empty @15:31:48.67 Setting up PCR for Mm -- writing /var/log/esp/Mm19may0915h.pcr @15:31:48.70 Building PCR Slug from extSrc with MM2 mix, PP3 primer @15:31:48.72 Priming with carrier @15:31:48.74 ATV.dial ARV @15:31:50.26 ARV.dial :carrier @15:31:50.61 AS.reconfigure ASnorm @15:31:50.67 AS.seek 0.499ml @15:31:58.22 -> AS.stop @15:31:58.23 AS.stop @15:31:58.34 Slide::Error in MFBPCR -- Analytical Syringe aborted at 0.146ml @15:31:58.40 Waiting to Resume from Checkpoint "Analytical Syringe aborted at 0.146ml" @15:31:58.43 BadNews.email "Analytical Syringe aborted at 0.146ml",Subject: "Slide::Error in MFBPCR" @15:32:57.74 -> 3.times{deconEluateRecoveryLine :extSrc} @15:32:57.76 deconEluateRecoveryLine extSrc @15:32:57.77 ATV.dial :waste @15:32:59.50 AS.seek 0ml @15:33:07.29 ATV.dial :air3 @15:33:08.14 AS.reconfigure ASslow1 @15:33:08.20 AS.seek 0.01ml @15:33:10.52 delay 2 seconds @15:33:12.54 ATV.dial :bleach @15:33:13.02 AS.seek 0.31ml @15:34:13.63 delay 3 seconds @15:34:16.65 ATV.dial :air3 @15:34:17.21 delay 3 seconds @15:34:20.28 AS.seek 0.335ml @15:34:25.61 delay 3 seconds @15:34:28.63 ATV.dial :bleach @15:34:29.25 AS.seek 0.32ml @15:34:32.57 delay 3 seconds @15:34:35.59 ATV.dial :extSrc @15:34:36.23 AS.seek 0ml @15:35:40.84 AFV.select :carrierSolution @15:35:40.97 delay 2 seconds @15:35:42.99 AS.reconfigure ASconfig @15:35:43.06 AS.seek 0.5ml @15:36:12.11 delay 1 second @15:36:13.13 AFV.select :holdingCoil @15:36:13.23 ATV.dial :extSrc @15:36:13.25 AS.reconfigure ASslow1 @15:36:13.32 AS.seek 0ml @15:37:54.14 AFV.select :carrierSolution @15:37:54.26 delay 2 seconds @15:37:56.28 AS.reconfigure ASconfig @15:37:56.34 AS.seek 0.5ml @15:38:25.40 delay 1 second @15:38:26.42 AFV.select :holdingCoil @15:38:26.52 ATV.dial :extSrc @15:38:26.54 AS.reconfigure ASslow1 @15:38:26.61 AS.seek 0ml @15:40:07.43 AFV.select :carrierSolution @15:40:07.54 delay 2 seconds @15:40:09.56 AS.reconfigure ASconfig @15:40:09.62 AS.seek 0.5ml @15:40:38.69 delay 1 second @15:40:39.71 AFV.select :holdingCoil @15:40:39.81 ATV.dial :extSrc @15:40:39.83 AS.reconfigure ASslow1 @15:40:39.90 AS.seek 0ml @15:42:20.74 AFV.select :carrierSolution @15:42:20.86 delay 2 seconds @15:42:22.88 AS.reconfigure ASconfig @15:42:22.95 AS.seek 0.5ml @15:42:42.79 Session Begun @15:42:46.70 -> showlog 100 @15:42:52.32 delay 1 second @15:42:53.41 AFV.select :holdingCoil @15:42:53.53 ATV.dial :extSrc @15:42:53.56 AS.reconfigure ASslow1 @15:42:53.64 AS.seek 0ml @15:44:34.49 ATV.dial :air2 @15:44:35.85 AS.reconfigure ASslow0 @15:44:35.93 AS.seek 0.5ml @15:45:26.54 ATV.dial :extSrc @15:45:28.07 AS.seek 0.02ml @15:46:16.66 delay 1 second @15:46:17.68 ATV.dial :waste @15:46:19.02 AS.reconfigure ASconfig @15:46:19.09 AS.seek :empty @15:46:20.60 deconEluateRecoveryLine extSrc @15:46:20.61 ATV.dial :waste @15:46:20.66 AS.reconfigure ASnorm @15:46:20.74 AS.seek 0ml @15:46:21.11 ATV.dial :air3 @15:46:21.97 AS.reconfigure ASslow1 @15:46:22.04 AS.seek 0.01ml @15:46:24.36 delay 2 seconds @15:46:26.38 ATV.dial :bleach @15:46:26.85 AS.seek 0.31ml @15:47:27.49 delay 3 seconds @15:47:30.51 ATV.dial :air3 @15:47:31.07 delay 3 seconds @15:47:34.14 AS.seek 0.335ml @15:47:39.47 delay 3 seconds @15:47:42.49 ATV.dial :bleach @15:47:43.11 AS.seek 0.32ml @15:47:46.46 delay 3 seconds @15:47:49.48 ATV.dial :extSrc @15:47:50.12 AS.seek 0ml @15:48:54.75 AFV.select :carrierSolution @15:48:54.89 delay 2 seconds @15:48:56.92 AS.reconfigure ASconfig @15:48:56.99 AS.seek 0.5ml @15:49:26.04 delay 1 second @15:49:27.06 AFV.select :holdingCoil @15:49:27.18 ATV.dial :extSrc @15:49:27.20 AS.reconfigure ASslow1 @15:49:27.29 AS.seek 0ml @15:51:08.16 AFV.select :carrierSolution @15:51:08.29 delay 2 seconds @15:51:10.31 AS.reconfigure ASconfig @15:51:10.38 AS.seek 0.5ml @15:51:39.44 delay 1 second @15:51:40.46 AFV.select :holdingCoil @15:51:40.58 ATV.dial :extSrc @15:51:40.60 AS.reconfigure ASslow1 @15:51:40.69 AS.seek 0ml @15:53:21.54 AFV.select :carrierSolution @15:53:21.67 delay 2 seconds @15:53:23.69 AS.reconfigure ASconfig @15:53:23.76 AS.seek 0.5ml @15:53:52.82 delay 1 second @15:53:53.84 AFV.select :holdingCoil @15:53:53.96 ATV.dial :extSrc @15:53:53.99 AS.reconfigure ASslow1 @15:53:54.07 AS.seek 0ml @15:55:34.93 AFV.select :carrierSolution @15:55:35.06 delay 2 seconds @15:55:37.08 AS.reconfigure ASconfig @15:55:37.15 AS.seek 0.5ml @15:56:06.22 delay 1 second @15:56:07.24 AFV.select :holdingCoil @15:56:07.36 ATV.dial :extSrc @15:56:07.38 AS.reconfigure ASslow1 @15:56:07.47 AS.seek 0ml @15:57:48.30 ATV.dial :air2 @15:57:49.66 AS.reconfigure ASslow0 @15:57:49.73 AS.seek 0.5ml @15:58:40.35 ATV.dial :extSrc @15:58:41.88 AS.seek 0.02ml @15:59:30.49 delay 1 second @15:59:31.51 ATV.dial :waste @15:59:32.83 AS.reconfigure ASconfig @15:59:32.90 AS.seek :empty @15:59:34.43 deconEluateRecoveryLine extSrc @15:59:34.44 ATV.dial :waste @15:59:34.49 AS.reconfigure ASnorm @15:59:34.57 AS.seek 0ml @15:59:34.94 ATV.dial :air3 @15:59:35.81 AS.reconfigure ASslow1 @15:59:35.88 AS.seek 0.01ml @15:59:38.30 delay 2 seconds @15:59:40.32 ATV.dial :bleach @15:59:40.79 AS.seek 0.31ml @16:00:41.46 delay 3 seconds @16:00:44.48 ATV.dial :air3 @16:00:45.04 delay 3 seconds @16:00:48.11 AS.seek 0.335ml @16:00:53.45 delay 3 seconds @16:00:56.47 ATV.dial :bleach @16:00:57.10 AS.seek 0.32ml @16:01:00.41 delay 3 seconds @16:01:03.43 ATV.dial :extSrc @16:01:04.07 AS.seek 0ml @16:02:08.70 AFV.select :carrierSolution @16:02:08.84 delay 2 seconds @16:02:10.87 AS.reconfigure ASconfig @16:02:10.93 AS.seek 0.5ml @16:02:39.97 delay 1 second @16:02:40.99 AFV.select :holdingCoil @16:02:41.11 ATV.dial :extSrc @16:02:41.13 AS.reconfigure ASslow1 @16:02:41.22 AS.seek 0ml @16:04:22.06 AFV.select :carrierSolution @16:04:22.20 delay 2 seconds @16:04:24.22 AS.reconfigure ASconfig @16:04:24.29 AS.seek 0.5ml @16:04:53.35 delay 1 second @16:04:54.37 AFV.select :holdingCoil @16:04:54.49 ATV.dial :extSrc @16:04:54.52 AS.reconfigure ASslow1 @16:04:54.61 AS.seek 0ml @16:06:35.49 AFV.select :carrierSolution @16:06:35.62 delay 2 seconds @16:06:37.64 AS.reconfigure ASconfig @16:06:37.71 AS.seek 0.5ml @16:07:06.76 delay 1 second @16:07:07.78 AFV.select :holdingCoil @16:07:07.90 ATV.dial :extSrc @16:07:07.92 AS.reconfigure ASslow1 @16:07:08.01 AS.seek 0ml @16:08:48.86 AFV.select :carrierSolution @16:08:48.99 delay 2 seconds @16:08:51.02 AS.reconfigure ASconfig @16:08:51.08 AS.seek 0.5ml @16:09:20.15 delay 1 second @16:09:21.17 AFV.select :holdingCoil @16:09:21.29 ATV.dial :extSrc @16:09:21.31 AS.reconfigure ASslow1 @16:09:21.40 AS.seek 0ml @16:11:02.24 ATV.dial :air2 @16:11:03.61 AS.reconfigure ASslow0 @16:11:03.68 AS.seek 0.5ml @16:11:54.27 ATV.dial :extSrc @16:11:55.80 AS.seek 0.02ml @16:12:44.40 delay 1 second @16:12:45.42 ATV.dial :waste @16:12:46.76 AS.reconfigure ASconfig @16:12:46.83 AS.seek :empty @16:12:52.22 -> exit @16:12:52.23 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @16:15:14.76 Session Begun @16:15:31.26 -> showlog 10 @16:15:54.68 -> exit @16:15:54.69 Session Ended @16:21:16.86CEST09-May-19
ESP2 v3.30 I2C Gateway v3.06 for core bus @16:21:18.55 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @16:21:19.00 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @16:21:24.69 Polling Can every 10:00 @16:21:26.45 SPE.coast @16:21:26.49 SPE.configure SPEconfig @16:21:26.57 AS.reconfigure ASconfig @16:21:26.62 AS.hold @16:21:26.68 AS.configure ASconfig @16:21:26.76 SS.configure SSconfig @16:21:26.84 CC.configure CCconfig @16:21:26.92 CS.configure CSconfig @16:21:27.00 PC.configure PCconfig @16:21:27.08 PS.configure PSconfig @16:21:27.19 Forearm.configure LooseForearm @16:21:27.27 Elbow.configure LooseElbow @16:21:27.71 SC.configure SCloose @16:21:27.79 SE.configure SEconfig @16:21:27.82 CH.coast @16:21:27.87 CH.configure CHconfig @16:21:27.90 PH.coast @16:21:27.95 PH.configure PHconfig @16:21:27.98 PCR.off @16:21:28.02 PCR.pidtemp= 50,0.1,0,300,25,25 @16:21:28.05 PCR.caltemp= 2.8814,0.0372,0 @16:21:28.08 All dwarf controllers running firmware version 3.75 @16:21:28.23 Cleanly exited at 16:20:51CEST09-May-19 @16:21:28.26 Loading /home/esp/esp2/mission/stdMERwithoutESPready2.rb mission @16:21:28.28 AFV.select :carrierSolution @16:21:28.38 delay 2 seconds @16:21:30.41 AS.seek 0.5ml @16:21:44.20 Session Begun @16:21:49.61 -> showlog 10 @16:21:59.51
delay 1 second @16:22:00.53 AFV.select :holdingCoil @16:22:00.64 ATV.dial :extSrc @16:22:01.99 AS.reconfigure ASslow1 @16:22:02.05 AS.seek 0.53ml @16:22:08.37 delay 5 seconds @16:22:13.46 AS.reconfigure ASslow2 @16:22:13.51 AS.seek 0.54ml @16:22:23.82 delay 20 seconds @16:22:43.90 AS.reconfigure ASslow1 @16:22:43.95 AS.seek 0.57ml @16:22:50.26 delay 5 seconds @16:22:55.34 AS.reconfigure ASslow2 @16:22:55.40 AS.seek 0.58ml @16:23:05.69 delay 20 seconds @16:23:25.76 AS.reconfigure ASslow1 @16:23:25.83 AS.seek 0.61ml @16:23:32.10 delay 5 seconds @16:23:37.18 AS.reconfigure ASslow2 @16:23:37.26 AS.seek 0.62ml @16:23:47.52 delay 20 seconds @16:24:07.60 AS.reconfigure ASslow1 @16:24:07.65 AS.seek 0.65ml @16:24:13.94 delay 5 seconds @16:24:19.02 AS.reconfigure ASslow2 @16:24:19.08 AS.seek 0.66ml @16:24:29.44 delay 20 seconds @16:24:49.47 AS.reconfigure ASslow1 @16:24:49.53 AS.seek 0.705ml @16:24:58.90 delay 10 seconds @16:25:08.93 ATV.dial :air1 @16:25:10.66 AS.seek 0.725ml @16:25:14.96 delay 3 seconds @16:25:17.99 ATV.dial :waste @16:25:18.55 AS.reconfigure ASslow0 @16:25:18.60 AS.seek :empty @16:26:32.05 Setting up PCR for Mm -- writing /var/log/esp/Mm19may0916h.pcr @16:26:32.08 Building PCR Slug from extSrc with MM2 mix, PP3 primer @16:26:32.10 Priming with carrier @16:26:32.13 ATV.dial ARV @16:26:33.65 ARV.dial :carrier @16:26:34.00 AS.reconfigure ASnorm @16:26:34.07 AS.seek 0.499ml @16:26:59.63 AFV.select :carrierSolution @16:26:59.75 delay 2 seconds @16:27:01.77 AS.reconfigure ASconfig @16:27:01.84 AS.seek 1ml @16:27:30.91 delay 1 second @16:27:31.93 AFV.select :holdingCoil @16:27:32.04 delay 1 second @16:27:33.06 ATV.dial :waste @16:27:34.77 AS.reconfigure ASnorm @16:27:34.83 AS.seek :empty @16:28:25.58 AFV.select :carrierSolution @16:28:25.70 delay 2 seconds @16:28:27.72 AS.reconfigure ASconfig @16:28:27.78 AS.seek 0.5ml @16:28:56.88 delay 1 second @16:28:57.90 AFV.select :holdingCoil @16:28:58.01 Building Slug @16:28:58.02 ATV.dial :ARV @16:28:59.70 delay 2 seconds @16:29:01.72 ARV.dial :air1 @16:29:02.23 delay 2 seconds @16:29:04.30 AS.reconfigure ASslow2 @16:29:04.37 AS.seek 0.506ml @16:29:10.85 delay 2 seconds @16:29:12.88 ARV.dial :MM2 @16:29:14.37 delay 2 seconds @16:29:16.40 Adding MM2 @16:29:16.49 AS.seek 0.524ml @16:29:34.93 delay 25 seconds @16:29:59.96 ARV.dial :PP3 @16:30:00.36 Adding PP3 @16:30:00.37 delay 2 seconds @16:30:02.45 AS.seek 0.53ml @16:30:08.71 delay 10 seconds @16:30:18.75 ARV.dial :air2 @16:30:19.16 adding ARV air @16:30:19.17 delay 2 seconds @16:30:21.25 AS.seek 0.536ml @16:30:27.71 delay 5 seconds @16:30:32.74 ARV.dial :carrier @16:30:34.10 delay 7 seconds @16:30:41.19 AS.seek 0.594ml @16:31:39.66 delay 5 seconds @16:31:44.69 ATV.dial :extSrc @16:31:45.24 delay 2 seconds @16:31:47.32 AS.seek 0.6ml @16:31:53.54 delay 4 seconds @16:31:57.57 ATV.dial ARV @16:31:58.12 delay 2 seconds @16:32:00.15 ARV.dial :carrier @16:32:00.16 delay 2 seconds @16:32:02.24 AS.seek 0.65ml @16:32:52.88 delay 4 seconds @16:32:56.91 ATV.dial PCR @16:32:57.68 ASV.dial :waste1 @16:32:57.77 ARV.dial :air1 @16:32:58.20 Positioning Slug by 0.324ml @16:32:58.29 AS.seek 0.326ml @16:38:24.26 delay 5 seconds @16:38:29.29 ATV.dial ARV @16:38:30.07 ASV.dial :PCRlock @16:38:30.48 delay 5 seconds @16:38:35.50 Running Mm Profile60C350 thermocycling @16:38:35.51 PCR.program Profile60C350 @16:38:35.67 Profile60C350 should complete in < 1:48:05 @16:38:35.68 PCR.run 2:20:06.25 @18:02:12.11 PCR.off @18:02:14.04 PCR for Mm completed -- closed /var/log/esp/Mm19may0916h.pcr @18:02:14.11 Cleaning PCR @18:02:14.12 AFV.select :carrierSolution @18:02:14.25 delay 2 seconds @18:02:16.27 AS.reconfigure ASconfig @18:02:16.33 AS.seek 0.5ml @18:02:26.63 delay 1 second @18:02:27.65 AFV.select :holdingCoil @18:02:27.76 ASV.dial :waste1 @18:02:28.15 ATV.dial :air3 @18:02:29.47 AS.reconfigure ASslow1 @18:02:29.53 AS.seek 0.51ml @18:02:31.83 delay 2 seconds @18:02:33.85 ATV.dial :bleach @18:02:34.47 AS.seek 0.81ml @18:03:35.07 delay 3 seconds @18:03:38.10 ATV.dial :air3 @18:03:38.65 delay 3 seconds @18:03:41.73 AS.seek 0.835ml @18:03:47.06 delay 3 seconds @18:03:50.09 ATV.dial :bleach @18:03:50.71 AS.seek 0.82ml @18:03:54.01 delay 3 seconds @18:03:57.04 ARV.dial :waste @18:03:57.65 delay 3 seconds @18:04:00.68 ATV.dial :ARV @18:04:01.58 AS.reconfigure ASconfig @18:04:01.64 AS.seek 0.47ml @18:04:22.04 AFV.select :carrierSolution @18:04:22.16 delay 2 seconds @18:04:24.18 AS.seek 1ml @18:04:54.93 delay 1 second @18:04:55.95 AFV.select :holdingCoil @18:04:56.08 delay 1 second @18:04:57.10 AS.reconfigure ASfast @18:04:57.16 AS.seek 0ml @18:05:24.52 AFV.select :carrierSolution @18:05:24.64 delay 2 seconds @18:05:26.66 AS.reconfigure ASconfig @18:05:26.72 AS.seek 1ml @18:06:24.93 delay 1 second @18:06:25.95 AFV.select :holdingCoil @18:06:26.06 delay 1 second @18:06:27.08 AS.reconfigure ASfast @18:06:27.14 AS.seek 0ml @18:06:54.51 AFV.select :carrierSolution @18:06:54.63 delay 2 seconds @18:06:56.65 AS.reconfigure ASconfig @18:06:56.71 AS.seek 1ml @18:07:54.47 delay 1 second @18:07:55.49 AFV.select :holdingCoil @18:07:55.60 delay 1 second @18:07:56.62 AS.reconfigure ASfast @18:07:56.68 AS.seek 0ml @18:08:24.04 ARV.dial between carrier and air1 @18:08:24.56 ATV.dial :air3 @18:08:25.86 AS.reconfigure ASslow1 @18:08:25.92 AS.seek 0.01ml @18:08:28.27 delay 2 seconds @18:08:30.30 ATV.dial :bleach @18:08:30.93 AS.seek 0.81ml @18:11:12.15 delay 3 seconds @18:11:15.18 ATV.dial :air3 @18:11:15.75 delay 3 seconds @18:11:18.82 AS.seek 0.835ml @18:11:24.14 delay 3 seconds @18:11:27.17 ATV.dial :bleach @18:11:27.80 AS.seek 0.82ml @18:11:31.11 delay 3 seconds @18:11:34.14 ATV.dial PCR @18:11:34.56 AS.reconfigure ASconfig @18:11:34.61 AS.seek :empty @18:12:22.10 PCR.program :bleachProfile @18:12:22.16 PCR program should complete in < 2:45 @18:12:22.17 PCR.run 10:00 @18:15:17.96 PCR.off @18:15:17.99 AFV.select :carrierSolution @18:15:18.11 delay 2 seconds @18:15:20.13 AS.seek 1ml @18:16:17.97 delay 1 second @18:16:18.99 AFV.select :holdingCoil @18:16:19.10 delay 1 second @18:16:20.12 AS.reconfigure ASfast @18:16:20.18 AS.seek 0ml @18:16:47.52 delay 3 seconds @18:16:50.54 AFV.select :carrierSolution @18:16:50.65 delay 2 seconds @18:16:52.67 AS.reconfigure ASconfig @18:16:52.74 AS.seek 1ml @18:17:50.54 delay 1 second @18:17:51.56 AFV.select :holdingCoil @18:17:51.68 delay 1 second @18:17:52.70 AS.reconfigure ASfast @18:17:52.76 AS.seek 0ml @18:18:20.11 delay 3 seconds @18:18:23.13 AFV.select :carrierSolution @18:18:23.24 delay 2 seconds @18:18:25.26 AS.reconfigure ASconfig @18:18:25.33 AS.seek 1ml @18:19:23.08 delay 1 second @18:19:24.10 AFV.select :holdingCoil @18:19:24.22 delay 1 second @18:19:25.24 AS.reconfigure ASfast @18:19:25.30 AS.seek 0ml @18:19:52.68 delay 3 seconds @18:19:55.70 AFV.select :carrierSolution @18:19:55.81 delay 2 seconds @18:19:57.83 AS.reconfigure ASconfig @18:19:57.89 AS.seek 1ml @18:20:55.69 delay 1 second @18:20:56.71 AFV.select :holdingCoil @18:20:56.82 delay 1 second @18:20:57.84 AS.reconfigure ASfast @18:20:57.90 AS.seek 0ml @18:21:25.26 delay 3 seconds @18:21:28.28 AFV.select :carrierSolution @18:21:28.39 delay 2 seconds @18:21:30.41 AS.reconfigure ASconfig @18:21:30.48 AS.seek 1ml @18:22:28.24 delay 1 second @18:22:29.26 AFV.select :holdingCoil @18:22:29.38 delay 1 second @18:22:30.40 AS.reconfigure ASnorm @18:22:30.46 AS.seek 0ml @18:23:21.18
AFV.select :carrierSolution @18:23:21.29 delay 2 seconds @18:23:23.31 AS.reconfigure ASconfig @18:23:23.37 AS.seek 0.5ml @18:23:52.44 delay 1 second @18:23:53.46 AFV.select :holdingCoil @18:23:53.58 ATV.dial :air1 @18:23:55.10 AS.reconfigure ASslow2 @18:23:55.15 AS.seek 0.51ml @18:24:05.46 delay 5 seconds @18:24:10.49 ATV.dial :extSrc @18:24:12.22 AS.seek 0.83ml @18:29:34.51 delay 10 seconds @18:29:44.54 ATV.dial :air1 @18:29:46.27 AS.reconfigure ASslow1 @18:29:46.32 AS.seek 0.86ml @18:29:52.65 ATV.dial :waste @18:29:53.22 AS.reconfigure ASconfig @18:29:53.27 AS.seek :empty @18:30:43.22 Setting up PCR for Mm -- writing /var/log/esp/Mm19may0918h.pcr @18:30:43.24 Building PCR Slug from extSrc with MM2 mix, PP3 primer @18:30:43.27 Priming with carrier @18:30:43.30 ATV.dial ARV @18:30:44.82 ARV.dial :carrier @18:30:45.17 AS.reconfigure ASnorm @18:30:45.24 AS.seek 0.499ml @18:31:10.82 AFV.select :carrierSolution @18:31:10.94 delay 2 seconds @18:31:12.96 AS.reconfigure ASconfig @18:31:13.03 AS.seek 1ml @18:31:42.11 delay 1 second @18:31:43.13 AFV.select :holdingCoil @18:31:43.24 delay 1 second @18:31:44.26 ATV.dial :waste @18:31:45.97 AS.reconfigure ASnorm @18:31:46.03 AS.seek :empty @18:32:36.77 AFV.select :carrierSolution @18:32:36.89 delay 2 seconds @18:32:38.91 AS.reconfigure ASconfig @18:32:38.97 AS.seek 0.5ml @18:33:08.10 delay 1 second @18:33:09.12 AFV.select :holdingCoil @18:33:09.23 Building Slug @18:33:09.24 ATV.dial :ARV @18:33:10.92 delay 2 seconds @18:33:12.94 ARV.dial :air1 @18:33:13.34 delay 2 seconds @18:33:15.42 AS.reconfigure ASslow2 @18:33:15.48 AS.seek 0.506ml @18:33:21.99 delay 2 seconds @18:33:24.02 ARV.dial :MM2 @18:33:25.49 delay 2 seconds @18:33:27.53 Adding MM2 @18:33:27.61 AS.seek 0.524ml @18:33:46.02 delay 25 seconds @18:34:11.05 ARV.dial :PP3 @18:34:11.45 Adding PP3 @18:34:11.46 delay 2 seconds @18:34:13.54 AS.seek 0.53ml @18:34:19.82 delay 10 seconds @18:34:29.86 ARV.dial :air2 @18:34:30.27 adding ARV air @18:34:30.28 delay 2 seconds @18:34:32.36 AS.seek 0.536ml @18:34:38.52 delay 5 seconds @18:34:43.55 ARV.dial :carrier @18:34:44.80 delay 7 seconds @18:34:51.89 AS.seek 0.594ml @18:35:50.36 delay 5 seconds @18:35:55.39 ATV.dial :extSrc @18:35:55.94 delay 2 seconds @18:35:58.02 AS.seek 0.6ml @18:36:04.19 delay 4 seconds @18:36:08.22 ATV.dial ARV @18:36:08.77 delay 2 seconds @18:36:10.80 ARV.dial :carrier @18:36:10.81 delay 2 seconds @18:36:12.89 AS.seek 0.65ml @18:37:03.63 delay 4 seconds @18:37:07.66 ATV.dial PCR @18:37:08.43 ASV.dial :waste1 @18:37:08.45 ARV.dial :air1 @18:37:08.87 Positioning Slug by 0.324ml @18:37:08.96 AS.seek 0.326ml @18:42:34.93 delay 5 seconds @18:42:39.96 ATV.dial ARV @18:42:40.74 ASV.dial :PCRlock @18:42:41.16 delay 5 seconds @18:42:46.18 Running Mm Profile60C350 thermocycling @18:42:46.19 PCR.program Profile60C350 @18:42:46.34 Profile60C350 should complete in < 1:48:05 @18:42:46.35 PCR.run 2:20:06.25 @20:06:17.40 PCR.off @20:06:19.60 PCR for Mm completed -- closed /var/log/esp/Mm19may0918h.pcr @20:06:19.68 Cleaning PCR @20:06:19.69 AFV.select :carrierSolution @20:06:19.82 delay 2 seconds @20:06:21.84 AS.reconfigure ASconfig @20:06:21.90 AS.seek 0.5ml @20:06:32.21 delay 1 second @20:06:33.23 AFV.select :holdingCoil @20:06:33.35 ASV.dial :waste1 @20:06:33.74 ATV.dial :air3 @20:06:35.05 AS.reconfigure ASslow1 @20:06:35.11 AS.seek 0.51ml @20:06:37.45 delay 2 seconds @20:06:39.48 ATV.dial :bleach @20:06:40.10 AS.seek 0.81ml @20:07:40.77 delay 3 seconds @20:07:43.80 ATV.dial :air3 @20:07:44.35 delay 3 seconds @20:07:47.43 AS.seek 0.835ml @20:07:52.77 delay 3 seconds @20:07:55.80 ATV.dial :bleach @20:07:56.43 AS.seek 0.82ml @20:07:59.75 delay 3 seconds @20:08:02.78 ARV.dial :waste @20:08:03.38 delay 3 seconds @20:08:06.41 ATV.dial :ARV @20:08:07.41 AS.reconfigure ASconfig @20:08:07.47 AS.seek 0.47ml @20:08:27.91 AFV.select :carrierSolution @20:08:28.03 delay 2 seconds @20:08:30.05 AS.seek 1ml @20:09:00.85 delay 1 second @20:09:01.87 AFV.select :holdingCoil @20:09:01.98 delay 1 second @20:09:03.00 AS.reconfigure ASfast @20:09:03.06 AS.seek 0ml @20:09:30.41 AFV.select :carrierSolution @20:09:30.53 delay 2 seconds @20:09:32.55 AS.reconfigure ASconfig @20:09:32.60 AS.seek 1ml @20:10:30.40 delay 1 second @20:10:31.42 AFV.select :holdingCoil @20:10:31.54 delay 1 second @20:10:32.56 AS.reconfigure ASfast @20:10:32.62 AS.seek 0ml @20:10:59.97 AFV.select :carrierSolution @20:11:00.09 delay 2 seconds @20:11:02.11 AS.reconfigure ASconfig @20:11:02.17 AS.seek 1ml @20:11:59.93 delay 1 second @20:12:00.95 AFV.select :holdingCoil @20:12:01.07 delay 1 second @20:12:02.09 AS.reconfigure ASfast @20:12:02.15 AS.seek 0ml @20:12:29.47 ARV.dial between carrier and air1 @20:12:29.98 ATV.dial :air3 @20:12:31.28 AS.reconfigure ASslow1 @20:12:31.34 AS.seek 0.01ml @20:12:33.68 delay 2 seconds @20:12:35.71 ATV.dial :bleach @20:12:36.33 AS.seek 0.81ml @20:15:17.46 delay 3 seconds @20:15:20.49 ATV.dial :air3 @20:15:21.05 delay 3 seconds @20:15:24.13 AS.seek 0.835ml @20:15:29.44 delay 3 seconds @20:15:32.47 ATV.dial :bleach @20:15:33.10 AS.seek 0.82ml @20:15:36.41 delay 3 seconds @20:15:39.44 ATV.dial PCR @20:15:39.86 AS.reconfigure ASconfig @20:15:39.91 AS.seek :empty @20:16:27.40 PCR.program :bleachProfile @20:16:27.46 PCR program should complete in < 2:45 @20:16:27.47 PCR.run 10:00 @20:19:23.23 PCR.off @20:19:23.26 AFV.select :carrierSolution @20:19:23.37 delay 2 seconds @20:19:25.39 AS.seek 1ml @20:20:23.22 delay 1 second @20:20:24.24 AFV.select :holdingCoil @20:20:24.36 delay 1 second @20:20:25.38 AS.reconfigure ASfast @20:20:25.44 AS.seek 0ml @20:20:52.77 delay 3 seconds @20:20:55.79 AFV.select :carrierSolution @20:20:55.90 delay 2 seconds @20:20:57.92 AS.reconfigure ASconfig @20:20:57.98 AS.seek 1ml @20:21:55.72 delay 1 second @20:21:56.74 AFV.select :holdingCoil @20:21:56.86 delay 1 second @20:21:57.88 AS.reconfigure ASfast @20:21:57.93 AS.seek 0ml @20:22:25.32 delay 3 seconds @20:22:28.34 AFV.select :carrierSolution @20:22:28.45 delay 2 seconds @20:22:30.47 AS.reconfigure ASconfig @20:22:30.53 AS.seek 1ml @20:23:28.35 delay 1 second @20:23:29.37 AFV.select :holdingCoil @20:23:29.49 delay 1 second @20:23:30.51 AS.reconfigure ASfast @20:23:30.57 AS.seek 0ml @20:23:57.93 delay 3 seconds @20:24:00.95 AFV.select :carrierSolution @20:24:01.06 delay 2 seconds @20:24:03.08 AS.reconfigure ASconfig @20:24:03.14 AS.seek 1ml @20:25:00.90 delay 1 second @20:25:01.92 AFV.select :holdingCoil @20:25:02.04 delay 1 second @20:25:03.06 AS.reconfigure ASfast @20:25:03.12 AS.seek 0ml @20:25:30.46 delay 3 seconds @20:25:33.48 AFV.select :carrierSolution @20:25:33.60 delay 2 seconds @20:25:35.62 AS.reconfigure ASconfig @20:25:35.69 AS.seek 1ml @20:26:33.46 delay 1 second @20:26:34.48 AFV.select :holdingCoil @20:26:34.59 delay 1 second @20:26:35.61 AS.reconfigure ASnorm @20:26:35.67 AS.seek 0ml @20:27:26.38
AFV.select :carrierSolution @20:27:26.49 delay 2 seconds @20:27:28.51 AS.reconfigure ASconfig @20:27:28.57 AS.seek 0.5ml @20:27:57.66 delay 1 second @20:27:58.67 AFV.select :holdingCoil @20:27:58.79 ATV.dial :air1 @20:28:00.30 AS.reconfigure ASslow1 @20:28:00.36 AS.seek 0.52ml @20:28:04.68 delay 5 seconds @20:28:09.71 ATV.dial :extSrc @20:28:11.53 AS.reconfigure ASslow2 @20:28:11.59 AS.seek 0.557ml @20:28:49.05 delay 10 seconds @20:28:59.09 ATV.dial :air1 @20:29:00.83 AS.reconfigure ASslow1 @20:29:00.88 AS.seek 0.587ml @20:29:07.24 ATV.dial :waste @20:29:07.81 AS.reconfigure ASconfig @20:29:07.86 AS.seek :empty @20:29:42.10 Setting up PCR for Mm -- writing /var/log/esp/Mm19may0920h.pcr @20:29:42.12 Building PCR Slug from extSrc with MM2 mix, PP3 primer @20:29:42.14 Priming with carrier @20:29:42.16 ATV.dial ARV @20:29:43.69 ARV.dial :carrier @20:29:44.05 AS.reconfigure ASnorm @20:29:44.12 AS.seek 0.499ml @20:30:09.66 AFV.select :carrierSolution @20:30:09.78 delay 2 seconds @20:30:11.80 AS.reconfigure ASconfig @20:30:11.87 AS.seek 1ml @20:30:40.94 delay 1 second @20:30:41.96 AFV.select :holdingCoil @20:30:42.07 delay 1 second @20:30:43.09 ATV.dial :waste @20:30:44.80 AS.reconfigure ASnorm @20:30:44.86 AS.seek :empty @20:31:35.61 AFV.select :carrierSolution @20:31:35.73 delay 2 seconds @20:31:37.75 AS.reconfigure ASconfig @20:31:37.82 AS.seek 0.5ml @20:32:06.94 delay 1 second @20:32:07.96 AFV.select :holdingCoil @20:32:08.07 Building Slug @20:32:08.08 ATV.dial :ARV @20:32:09.77 delay 2 seconds @20:32:11.79 ARV.dial :air1 @20:32:12.21 delay 2 seconds @20:32:14.28 AS.reconfigure ASslow2 @20:32:14.34 AS.seek 0.506ml @20:32:20.54 delay 2 seconds @20:32:22.57 ARV.dial :MM2 @20:32:24.06 delay 2 seconds @20:32:26.08 Adding MM2 @20:32:26.20 AS.seek 0.524ml @20:32:44.66 delay 25 seconds @20:33:09.69 ARV.dial :PP3 @20:33:10.09 Adding PP3 @20:33:10.10 delay 2 seconds @20:33:12.18 AS.seek 0.53ml @20:33:18.65 delay 10 seconds @20:33:28.69 ARV.dial :air2 @20:33:29.07 adding ARV air @20:33:29.09 delay 2 seconds @20:33:31.17 AS.seek 0.536ml @20:33:37.55 delay 5 seconds @20:33:42.58 ARV.dial :carrier @20:33:43.86 delay 7 seconds @20:33:50.95 AS.seek 0.594ml @20:34:49.43 delay 5 seconds @20:34:54.46 ATV.dial :extSrc @20:34:55.00 delay 2 seconds @20:34:57.08 AS.seek 0.6ml @20:35:03.36 delay 4 seconds @20:35:07.39 ATV.dial ARV @20:35:07.94 delay 2 seconds @20:35:09.97 ARV.dial :carrier @20:35:09.98 delay 2 seconds @20:35:12.06 AS.seek 0.65ml @20:36:02.69 delay 4 seconds @20:36:06.72 ATV.dial PCR @20:36:07.49 ASV.dial :waste1 @20:36:07.51 ARV.dial :air1 @20:36:07.93 Positioning Slug by 0.324ml @20:36:08.02 AS.seek 0.326ml @20:41:33.97 delay 5 seconds @20:41:39.00 ATV.dial ARV @20:41:39.77 ASV.dial :PCRlock @20:41:40.19 delay 5 seconds @20:41:45.21 Running Mm Profile60C350 thermocycling @20:41:45.22 PCR.program Profile60C350 @20:41:45.38 Profile60C350 should complete in < 1:48:05 @20:41:45.39 PCR.run 2:20:06.25 @22:05:09.41 PCR.off @22:05:11.39 PCR for Mm completed -- closed /var/log/esp/Mm19may0920h.pcr @22:05:11.47 Cleaning PCR @22:05:11.48 AFV.select :carrierSolution @22:05:11.60 delay 2 seconds @22:05:13.62 AS.reconfigure ASconfig @22:05:13.68 AS.seek 0.5ml @22:05:23.99 delay 1 second @22:05:25.01 AFV.select :holdingCoil @22:05:25.12 ASV.dial :waste1 @22:05:25.51 ATV.dial :air3 @22:05:26.82 AS.reconfigure ASslow1 @22:05:26.88 AS.seek 0.51ml @22:05:29.25 delay 2 seconds @22:05:31.28 ATV.dial :bleach @22:05:31.89 AS.seek 0.81ml @22:06:32.52 delay 3 seconds @22:06:35.55 ATV.dial :air3 @22:06:36.10 delay 3 seconds @22:06:39.18 AS.seek 0.835ml @22:06:44.49 delay 3 seconds @22:06:47.52 ATV.dial :bleach @22:06:48.13 AS.seek 0.82ml @22:06:51.44 delay 3 seconds @22:06:54.47 ARV.dial :waste @22:06:55.08 delay 3 seconds @22:06:58.11 ATV.dial :ARV @22:06:59.02 AS.reconfigure ASconfig @22:06:59.08 AS.seek 0.47ml @22:07:19.49 AFV.select :carrierSolution @22:07:19.61 delay 2 seconds @22:07:22.01 AS.seek 1ml @22:07:52.79 delay 1 second @22:07:53.81 AFV.select :holdingCoil @22:07:53.92 delay 1 second @22:07:54.94 AS.reconfigure ASfast @22:07:55.01 AS.seek 0ml @22:08:22.35 AFV.select :carrierSolution @22:08:22.47 delay 2 seconds @22:08:24.49 AS.reconfigure ASconfig @22:08:24.55 AS.seek 1ml @22:09:22.32 delay 1 second @22:09:23.34 AFV.select :holdingCoil @22:09:23.45 delay 1 second @22:09:24.47 AS.reconfigure ASfast @22:09:24.53 AS.seek 0ml @22:09:51.88 AFV.select :carrierSolution @22:09:52.00 delay 2 seconds @22:09:54.02 AS.reconfigure ASconfig @22:09:54.08 AS.seek 1ml @22:10:51.88 delay 1 second @22:10:52.90 AFV.select :holdingCoil @22:10:53.01 delay 1 second @22:10:54.03 AS.reconfigure ASfast @22:10:54.09 AS.seek 0ml @22:11:21.44 ARV.dial between carrier and air1 @22:11:21.95 ATV.dial :air3 @22:11:23.27 AS.reconfigure ASslow1 @22:11:23.34 AS.seek 0.01ml @22:11:25.68 delay 2 seconds @22:11:27.71 ATV.dial :bleach @22:11:28.33 AS.seek 0.81ml @22:14:09.47 delay 3 seconds @22:14:12.50 ATV.dial :air3 @22:14:13.05 delay 3 seconds @22:14:16.13 AS.seek 0.835ml @22:14:21.47 delay 3 seconds @22:14:24.50 ATV.dial :bleach @22:14:25.11 AS.seek 0.82ml @22:14:28.40 delay 3 seconds @22:14:31.43 ATV.dial PCR @22:14:31.86 AS.reconfigure ASconfig @22:14:31.91 AS.seek :empty @22:15:19.38 PCR.program :bleachProfile @22:15:19.44 PCR program should complete in < 2:45 @22:15:19.45 PCR.run 10:00 @22:18:15.23 PCR.off @22:18:15.26 AFV.select :carrierSolution @22:18:15.37 delay 2 seconds @22:18:17.40 AS.seek 1ml @22:19:15.30 delay 1 second @22:19:16.32 AFV.select :holdingCoil @22:19:16.43 delay 1 second @22:19:17.45 AS.reconfigure ASfast @22:19:17.51 AS.seek 0ml @22:19:44.85 delay 3 seconds @22:19:47.87 AFV.select :carrierSolution @22:19:47.98 delay 2 seconds @22:19:50.00 AS.reconfigure ASconfig @22:19:50.07 AS.seek 1ml @22:20:47.86 delay 1 second @22:20:48.88 AFV.select :holdingCoil @22:20:48.99 delay 1 second @22:20:50.01 AS.reconfigure ASfast @22:20:50.07 AS.seek 0ml @22:21:17.43 delay 3 seconds @22:21:20.45 AFV.select :carrierSolution @22:21:20.56 delay 2 seconds @22:21:22.58 AS.reconfigure ASconfig @22:21:22.64 AS.seek 1ml @22:22:20.40 delay 1 second @22:22:21.42 AFV.select :holdingCoil @22:22:21.53 delay 1 second @22:22:22.55 AS.reconfigure ASfast @22:22:22.61 AS.seek 0ml @22:22:49.96 delay 3 seconds @22:22:52.98 AFV.select :carrierSolution @22:22:53.09 delay 2 seconds @22:22:55.11 AS.reconfigure ASconfig @22:22:55.18 AS.seek 1ml @22:23:52.91 delay 1 second @22:23:53.93 AFV.select :holdingCoil @22:23:54.04 delay 1 second @22:23:55.06 AS.reconfigure ASfast @22:23:55.12 AS.seek 0ml @22:24:22.47 delay 3 seconds @22:24:25.49 AFV.select :carrierSolution @22:24:25.60 delay 2 seconds @22:24:27.62 AS.reconfigure ASconfig @22:24:27.68 AS.seek 1ml @22:25:25.47 delay 1 second @22:25:26.49 AFV.select :holdingCoil @22:25:26.60 delay 1 second @22:25:27.62 AS.reconfigure ASnorm @22:25:27.67 AS.seek 0ml @22:26:18.40
AFV.select :carrierSolution @22:26:18.51 delay 2 seconds @22:26:20.53 AS.reconfigure ASconfig @22:26:20.58 AS.seek 0.5ml @22:26:49.68 delay 1 second @22:26:50.70 AFV.select :holdingCoil @22:26:50.82 ATV.dial :air1 @22:26:52.33 AS.reconfigure ASslow2 @22:26:52.38 AS.seek 0.506ml @22:26:58.65 ATV.dial :extSrc @22:27:00.38 AS.seek 0.512ml @22:27:06.97 delay 5 seconds @22:27:12.00 ATV.dial :air1 @22:27:13.74 AS.reconfigure ASslow1 @22:27:13.80 AS.seek 0.542ml @22:27:20.08 ATV.dial :waste @22:27:20.64 AS.reconfigure ASconfig @22:27:20.69 AS.seek :empty @22:27:52.44 Setting up PCR for Mm -- writing /var/log/esp/Mm19may0922h.pcr @22:27:52.46 Building PCR Slug from extSrc with MM2 mix, PP3 primer @22:27:52.49 Priming with carrier @22:27:52.50 ATV.dial ARV @22:27:54.02 ARV.dial :carrier @22:27:54.39 AS.reconfigure ASnorm @22:27:54.45 AS.seek 0.499ml @22:28:20.01 AFV.select :carrierSolution @22:28:20.14 delay 2 seconds @22:28:22.16 AS.reconfigure ASconfig @22:28:22.23 AS.seek 1ml @22:28:51.29 delay 1 second @22:28:52.32 AFV.select :holdingCoil @22:28:52.42 delay 1 second @22:28:53.44 ATV.dial :waste @22:28:55.14 AS.reconfigure ASnorm @22:28:55.20 AS.seek :empty @22:29:45.94 AFV.select :carrierSolution @22:29:46.07 delay 2 seconds @22:29:48.09 AS.reconfigure ASconfig @22:29:48.15 AS.seek 0.5ml @22:30:17.26 delay 1 second @22:30:18.28 AFV.select :holdingCoil @22:30:18.39 Building Slug @22:30:18.40 ATV.dial :ARV @22:30:20.09 delay 2 seconds @22:30:22.11 ARV.dial :air1 @22:30:22.52 delay 2 seconds @22:30:24.59 AS.reconfigure ASslow2 @22:30:24.65 AS.seek 0.506ml @22:30:31.01 delay 2 seconds @22:30:33.04 ARV.dial :MM2 @22:30:34.53 delay 2 seconds @22:30:36.55 Adding MM2 @22:30:36.63 AS.seek 0.524ml @22:30:55.05 delay 25 seconds @22:31:20.08 ARV.dial :PP3 @22:31:20.48 Adding PP3 @22:31:20.49 delay 2 seconds @22:31:22.57 AS.seek 0.53ml @22:31:28.80 delay 10 seconds @22:31:38.84 ARV.dial :air2 @22:31:39.23 adding ARV air @22:31:39.24 delay 2 seconds @22:31:41.32 AS.seek 0.536ml @22:31:47.68 delay 5 seconds @22:31:52.71 ARV.dial :carrier @22:31:53.97 delay 7 seconds @22:32:01.06 AS.seek 0.594ml @22:32:59.61 delay 5 seconds @22:33:04.64 ATV.dial :extSrc @22:33:05.21 delay 2 seconds @22:33:07.29 AS.seek 0.6ml @22:33:13.52 delay 4 seconds @22:33:17.55 ATV.dial ARV @22:33:18.10 delay 2 seconds @22:33:20.13 ARV.dial :carrier @22:33:20.14 delay 2 seconds @22:33:22.22 AS.seek 0.65ml @22:34:12.71 delay 4 seconds @22:34:16.74 ATV.dial PCR @22:34:17.52 ASV.dial :waste1 @22:34:17.54 ARV.dial :air1 @22:34:18.06 Positioning Slug by 0.324ml @22:34:18.15 AS.seek 0.326ml @22:39:44.19 delay 5 seconds @22:39:49.22 ATV.dial ARV @22:39:50.01 ASV.dial :PCRlock @22:39:50.42 delay 5 seconds @22:39:55.44 Running Mm Profile60C350 thermocycling @22:39:55.45 PCR.program Profile60C350 @22:39:55.60 Profile60C350 should complete in < 1:48:05 @22:39:55.61 PCR.run 2:20:06.25 @00:03:18.62CEST10-May-19 PCR.off @00:03:20.60 PCR for Mm completed -- closed /var/log/esp/Mm19may0922h.pcr @00:03:20.67 Cleaning PCR @00:03:20.68 AFV.select :carrierSolution @00:03:20.80 delay 2 seconds @00:03:22.82 AS.reconfigure ASconfig @00:03:22.88 AS.seek 0.5ml @00:03:33.19 delay 1 second @00:03:34.21 AFV.select :holdingCoil @00:03:34.32 ASV.dial :waste1 @00:03:34.71 ATV.dial :air3 @00:03:36.02 AS.reconfigure ASslow1 @00:03:36.08 AS.seek 0.51ml @00:03:38.37 delay 2 seconds @00:03:40.39 ATV.dial :bleach @00:03:41.00 AS.seek 0.81ml @00:04:41.57 delay 3 seconds @00:04:44.60 ATV.dial :air3 @00:04:45.15 delay 3 seconds @00:04:48.23 AS.seek 0.835ml @00:04:53.51 delay 3 seconds @00:04:56.54 ATV.dial :bleach @00:04:57.14 AS.seek 0.82ml @00:05:00.47 delay 3 seconds @00:05:03.50 ARV.dial :waste @00:05:04.10 delay 3 seconds @00:05:07.13 ATV.dial :ARV @00:05:08.03 AS.reconfigure ASconfig @00:05:08.09 AS.seek 0.47ml @00:05:28.49 AFV.select :carrierSolution @00:05:28.61 delay 2 seconds @00:05:30.63 AS.seek 1ml @00:06:01.40 delay 1 second @00:06:02.42 AFV.select :holdingCoil @00:06:02.53 delay 1 second @00:06:03.55 AS.reconfigure ASfast @00:06:03.62 AS.seek 0ml @00:06:30.97 AFV.select :carrierSolution @00:06:31.41 delay 2 seconds @00:06:33.49 AS.reconfigure ASconfig @00:06:33.55 AS.seek 1ml @00:07:31.40 delay 1 second @00:07:32.42 AFV.select :holdingCoil @00:07:32.53 delay 1 second @00:07:33.55 AS.reconfigure ASfast @00:07:33.61 AS.seek 0ml @00:08:00.96 AFV.select :carrierSolution @00:08:01.08 delay 2 seconds @00:08:03.10 AS.reconfigure ASconfig @00:08:03.16 AS.seek 1ml @00:09:00.93 delay 1 second @00:09:01.95 AFV.select :holdingCoil @00:09:02.06 delay 1 second @00:09:03.08 AS.reconfigure ASfast @00:09:03.15 AS.seek 0ml @00:09:30.51 ARV.dial between carrier and air1 @00:09:31.03 ATV.dial :air3 @00:09:32.33 AS.reconfigure ASslow1 @00:09:32.39 AS.seek 0.01ml @00:09:34.69 delay 2 seconds @00:09:36.72 ATV.dial :bleach @00:09:37.36 AS.seek 0.81ml @00:12:18.49 delay 3 seconds @00:12:21.52 ATV.dial :air3 @00:12:22.07 delay 3 seconds @00:12:25.15 AS.seek 0.835ml @00:12:30.47 delay 3 seconds @00:12:33.50 ATV.dial :bleach @00:12:34.11 AS.seek 0.82ml @00:12:37.40 delay 3 seconds @00:12:40.43 ATV.dial PCR @00:12:40.86 AS.reconfigure ASconfig @00:12:40.91 AS.seek :empty @00:13:28.40 PCR.program :bleachProfile @00:13:28.46 PCR program should complete in < 2:45 @00:13:28.47 PCR.run 10:00 @00:16:24.26 PCR.off @00:16:24.29 AFV.select :carrierSolution @00:16:24.41 delay 2 seconds @00:16:26.43 AS.seek 1ml @00:17:24.27 delay 1 second @00:17:25.29 AFV.select :holdingCoil @00:17:25.40 delay 1 second @00:17:26.42 AS.reconfigure ASfast @00:17:26.48 AS.seek 0ml @00:17:53.83 delay 3 seconds @00:17:56.85 AFV.select :carrierSolution @00:17:56.96 delay 2 seconds @00:17:58.98 AS.reconfigure ASconfig @00:17:59.04 AS.seek 1ml @00:18:56.79 delay 1 second @00:18:57.81 AFV.select :holdingCoil @00:18:57.92 delay 1 second @00:18:58.94 AS.reconfigure ASfast @00:18:59.00 AS.seek 0ml @00:19:26.33 delay 3 seconds @00:19:29.35 AFV.select :carrierSolution @00:19:29.46 delay 2 seconds @00:19:31.48 AS.reconfigure ASconfig @00:19:31.54 AS.seek 1ml @00:20:29.33 delay 1 second @00:20:30.35 AFV.select :holdingCoil @00:20:30.46 delay 1 second @00:20:31.48 AS.reconfigure ASfast @00:20:31.54 AS.seek 0ml @00:20:58.90 delay 3 seconds @00:21:01.92 AFV.select :carrierSolution @00:21:02.03 delay 2 seconds @00:21:04.05 AS.reconfigure ASconfig @00:21:04.12 AS.seek 1ml @00:22:01.88 delay 1 second @00:22:02.90 AFV.select :holdingCoil @00:22:03.01 delay 1 second @00:22:04.03 AS.reconfigure ASfast @00:22:04.09 AS.seek 0ml @00:22:31.44 delay 3 seconds @00:22:34.46 AFV.select :carrierSolution @00:22:34.57 delay 2 seconds @00:22:36.59 AS.reconfigure ASconfig @00:22:36.65 AS.seek 1ml @00:23:34.44 delay 1 second @00:23:35.46 AFV.select :holdingCoil @00:23:35.57 delay 1 second @00:23:36.59 AS.reconfigure ASnorm @00:23:36.65 AS.seek 0ml @00:24:27.37
AFV.select :carrierSolution @00:24:27.48 delay 2 seconds @00:24:29.50 AS.reconfigure ASconfig @00:24:29.55 AS.seek 0.5ml @00:24:58.60 delay 1 second @00:24:59.62 AFV.select :holdingCoil @00:24:59.74 ATV.dial :air1 @00:25:01.25 AS.reconfigure ASslow1 @00:25:01.30 AS.seek 0.52ml @00:25:05.65 delay 5 seconds @00:25:10.68 ATV.dial :extSrc @00:25:12.39 AS.reconfigure ASslow2 @00:25:12.44 AS.seek 0.538ml @00:25:30.71 delay 10 seconds @00:25:40.74 ATV.dial :air1 @00:25:42.47 AS.reconfigure ASslow1 @00:25:42.53 AS.seek 0.568ml @00:25:48.83 ATV.dial :waste @00:25:49.77 AS.reconfigure ASconfig @00:25:49.82 AS.seek :empty @00:26:22.83 deconEluateRecoveryLine extSrc @00:26:22.84 ATV.dial :waste @00:26:22.88 AS.reconfigure ASnorm @00:26:22.94 AS.seek 0ml @00:26:23.32 ATV.dial :air3 @00:26:24.02 AS.reconfigure ASslow1 @00:26:24.08 AS.seek 0.01ml @00:26:26.35 delay 2 seconds @00:26:28.38 ATV.dial :bleach @00:26:28.85 AS.seek 0.31ml @00:27:29.47 delay 3 seconds @00:27:32.50 ATV.dial :air3 @00:27:33.05 delay 3 seconds @00:27:36.13 AS.seek 0.335ml @00:27:41.44 delay 3 seconds @00:27:44.47 ATV.dial :bleach @00:27:45.08 AS.seek 0.32ml @00:27:48.37 delay 3 seconds @00:27:51.40 ATV.dial :extSrc @00:27:52.03 AS.seek 0ml @00:28:56.65 AFV.select :carrierSolution @00:28:56.77 delay 2 seconds @00:28:58.79 AS.reconfigure ASconfig @00:28:58.85 AS.seek 0.5ml @00:29:27.88 delay 1 second @00:29:28.90 AFV.select :holdingCoil @00:29:29.01 ATV.dial :extSrc @00:29:29.03 AS.reconfigure ASslow1 @00:29:29.10 AS.seek 0ml @00:31:09.96 AFV.select :carrierSolution @00:31:10.08 delay 2 seconds @00:31:12.10 AS.reconfigure ASconfig @00:31:12.16 AS.seek 0.5ml @00:31:41.19 delay 1 second @00:31:42.21 AFV.select :holdingCoil @00:31:42.32 ATV.dial :extSrc @00:31:42.34 AS.reconfigure ASslow1 @00:31:42.41 AS.seek 0ml @00:33:23.24 AFV.select :carrierSolution @00:33:23.36 delay 2 seconds @00:33:25.38 AS.reconfigure ASconfig @00:33:25.44 AS.seek 0.5ml @00:33:54.47 delay 1 second @00:33:55.49 AFV.select :holdingCoil @00:33:55.60 ATV.dial :extSrc @00:33:55.62 AS.reconfigure ASslow1 @00:33:55.69 AS.seek 0ml @00:35:36.52 AFV.select :carrierSolution @00:35:36.64 delay 2 seconds @00:35:38.66 AS.reconfigure ASconfig @00:35:38.72 AS.seek 0.5ml @00:36:07.76 delay 1 second @00:36:08.78 AFV.select :holdingCoil @00:36:08.89 ATV.dial :extSrc @00:36:08.91 AS.reconfigure ASslow1 @00:36:08.98 AS.seek 0ml @00:37:49.80 ATV.dial :air2 @00:37:51.16 AS.reconfigure ASslow0 @00:37:51.21 AS.seek 0.5ml @00:38:41.83 ATV.dial :extSrc @00:38:43.33 AS.seek 0.02ml @00:39:31.94 delay 1 second @00:39:32.97 ATV.dial :waste @00:39:34.27 AS.reconfigure ASconfig @00:39:34.32 AS.seek :empty @00:39:35.83 deconEluateRecoveryLine extSrc ATV.dial :waste @00:39:35.86 AS.reconfigure ASnorm @00:39:35.93 AS.seek 0ml @00:39:36.27 ATV.dial :air3 @00:39:37.14 AS.reconfigure ASslow1 @00:39:37.20 AS.seek 0.01ml @00:39:39.46 delay 2 seconds @00:39:41.49 ATV.dial :bleach @00:39:41.94 AS.seek 0.31ml @00:40:42.54 delay 3 seconds @00:40:45.57 ATV.dial :air3 @00:40:46.12 delay 3 seconds @00:40:49.20 AS.seek 0.335ml @00:40:54.51 delay 3 seconds @00:40:57.54 ATV.dial :bleach @00:40:58.18 AS.seek 0.32ml @00:41:01.47 delay 3 seconds @00:41:04.50 ATV.dial :extSrc @00:41:05.13 AS.seek 0ml @00:42:09.77 AFV.select :carrierSolution @00:42:09.89 delay 2 seconds @00:42:11.91 AS.reconfigure ASconfig @00:42:11.97 AS.seek 0.5ml @00:42:41.01 delay 1 second @00:42:42.03 AFV.select :holdingCoil @00:42:42.14 ATV.dial :extSrc @00:42:42.16 AS.reconfigure ASslow1 @00:42:42.23 AS.seek 0ml @00:44:23.04 AFV.select :carrierSolution @00:44:23.16 delay 2 seconds @00:44:25.18 AS.reconfigure ASconfig @00:44:25.23 AS.seek 0.5ml @00:44:54.27 delay 1 second @00:44:55.29 AFV.select :holdingCoil @00:44:55.40 ATV.dial :extSrc @00:44:55.42 AS.reconfigure ASslow1 @00:44:55.49 AS.seek 0ml @00:46:36.33 AFV.select :carrierSolution @00:46:36.45 delay 2 seconds @00:46:38.47 AS.reconfigure ASconfig @00:46:38.52 AS.seek 0.5ml @00:47:07.58 delay 1 second @00:47:08.60 AFV.select :holdingCoil @00:47:09.00 ATV.dial :extSrc @00:47:09.09 AS.reconfigure ASslow1 @00:47:09.15 AS.seek 0ml @00:48:49.97 AFV.select :carrierSolution @00:48:50.09 delay 2 seconds @00:48:52.11 AS.reconfigure ASconfig @00:48:52.17 AS.seek 0.5ml @00:49:21.22 delay 1 second @00:49:22.24 AFV.select :holdingCoil @00:49:22.35 ATV.dial :extSrc @00:49:22.37 AS.reconfigure ASslow1 @00:49:22.44 AS.seek 0ml @00:51:03.26 ATV.dial :air2 @00:51:04.61 AS.reconfigure ASslow0 @00:51:04.67 AS.seek 0.5ml @00:51:55.27 ATV.dial :extSrc @00:51:56.78 AS.seek 0.02ml @00:52:45.37 delay 1 second @00:52:46.40 ATV.dial :waste @00:52:47.72 AS.reconfigure ASconfig @00:52:47.77 AS.seek :empty @00:52:49.30 deconEluateRecoveryLine extSrc ATV.dial :waste @00:52:49.34 AS.reconfigure ASnorm @00:52:49.40 AS.seek 0ml @00:52:49.76 ATV.dial :air3 @00:52:50.61 AS.reconfigure ASslow1 @00:52:50.67 AS.seek 0.01ml @00:52:52.87 delay 2 seconds @00:52:54.90 ATV.dial :bleach @00:52:55.36 AS.seek 0.31ml @00:53:55.96 delay 3 seconds @00:53:58.99 ATV.dial :air3 @00:53:59.54 delay 3 seconds @00:54:02.62 AS.seek 0.335ml @00:54:07.94 delay 3 seconds @00:54:10.97 ATV.dial :bleach @00:54:11.58 AS.seek 0.32ml @00:54:14.88 delay 3 seconds @00:54:17.91 ATV.dial :extSrc @00:54:18.55 AS.seek 0ml @00:55:23.16 AFV.select :carrierSolution @00:55:23.28 delay 2 seconds @00:55:25.30 AS.reconfigure ASconfig @00:55:25.36 AS.seek 0.5ml @00:55:54.40 delay 1 second @00:55:55.42 AFV.select :holdingCoil @00:55:55.53 ATV.dial :extSrc @00:55:55.55 AS.reconfigure ASslow1 @00:55:55.62 AS.seek 0ml @00:57:36.43 AFV.select :carrierSolution @00:57:36.55 delay 2 seconds @00:57:38.57 AS.reconfigure ASconfig @00:57:38.63 AS.seek 0.5ml @00:58:07.65 delay 1 second @00:58:08.67 AFV.select :holdingCoil @00:58:08.78 ATV.dial :extSrc @00:58:08.80 AS.reconfigure ASslow1 @00:58:08.87 AS.seek 0ml @00:59:49.72 AFV.select :carrierSolution @00:59:49.84 delay 2 seconds @00:59:51.86 AS.reconfigure ASconfig @00:59:51.92 AS.seek 0.5ml @01:00:20.94 delay 1 second @01:00:21.96 AFV.select :holdingCoil @01:00:22.07 ATV.dial :extSrc @01:00:22.09 AS.reconfigure ASslow1 @01:00:22.16 AS.seek 0ml @01:02:03.01 AFV.select :carrierSolution @01:02:03.13 delay 2 seconds @01:02:05.15 AS.reconfigure ASconfig @01:02:05.21 AS.seek 0.5ml @01:02:34.24 delay 1 second @01:02:35.26 AFV.select :holdingCoil @01:02:35.37 ATV.dial :extSrc @01:02:35.39 AS.reconfigure ASslow1 @01:02:35.46 AS.seek 0ml @01:04:16.29 ATV.dial :air2 @01:04:17.64 AS.reconfigure ASslow0 @01:04:17.69 AS.seek 0.5ml @01:05:08.27 ATV.dial :extSrc @01:05:09.78 AS.seek 0.02ml @01:05:58.38 delay 1 second @01:05:59.41 ATV.dial :waste @01:06:00.73 AS.reconfigure ASconfig @01:06:00.78 AS.seek :empty @01:06:02.34 Session Ended @13:33:03.08CEST10-May-19
ESP2 v3.30 I2C Gateway v3.06 for core bus @13:33:04.79 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @13:33:05.04 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @13:33:09.29 Polling Can every 10:00 @13:33:11.04 SPE.coast @13:33:11.08 SPE.configure SPEconfig @13:33:11.16 AS.reconfigure ASconfig @13:33:11.20 AS.hold @13:33:11.26 AS.configure ASconfig @13:33:11.34 SS.configure SSconfig @13:33:11.42 CC.configure CCconfig @13:33:11.50 CS.configure CSconfig @13:33:11.58 PC.configure PCconfig @13:33:11.66 PS.configure PSconfig @13:33:11.76 Forearm.configure LooseForearm @13:33:11.84 Elbow.configure LooseElbow @13:33:12.27 SC.configure SCloose @13:33:12.35 SE.configure SEconfig @13:33:12.39 CH.coast @13:33:12.43 CH.configure CHconfig @13:33:12.46 PH.coast @13:33:12.51 PH.configure PHconfig @13:33:12.54 PCR.off @13:33:12.57 PCR.pidtemp= 50,0.1,0,300,25,25 @13:33:12.60 PCR.caltemp= 2.8814,0.0372,0 @13:33:12.63 All dwarf controllers running firmware version 3.75 @13:33:12.80 Cleanly exited at 13:29:58CEST10-May-19 @13:33:12.83 Loading /home/esp/esp2/mission/stdMERwithoutESPready2.rb mission @13:33:12.85 AFV.select :carrierSolution @13:33:12.95 delay 2 seconds @13:33:14.98 AS.seek 0.5ml @13:33:16.54 Session Begun @13:33:44.06
delay 1 second @13:33:45.08 AFV.select :holdingCoil @13:33:45.20 ATV.dial :extSrc @13:33:46.70 AS.reconfigure ASslow1 @13:33:46.76 AS.seek 0.53ml @13:33:53.08 delay 5 seconds @13:33:58.16 AS.reconfigure ASslow2 @13:33:58.22 AS.seek 0.54ml @13:34:08.45 delay 20 seconds @13:34:28.52 AS.reconfigure ASslow1 @13:34:28.58 AS.seek 0.57ml @13:34:34.86 delay 5 seconds @13:34:39.94 AS.reconfigure ASslow2 @13:34:40.00 AS.seek 0.58ml @13:34:50.28 delay 20 seconds @13:35:10.35 AS.reconfigure ASslow1 @13:35:10.41 AS.seek 0.61ml @13:35:16.72 delay 5 seconds @13:35:21.80 AS.reconfigure ASslow2 @13:35:21.86 AS.seek 0.62ml @13:35:32.39 delay 20 seconds @13:35:52.46 AS.reconfigure ASslow1 @13:35:52.52 AS.seek 0.65ml @13:35:58.89 delay 5 seconds @13:36:03.97 AS.reconfigure ASslow2 @13:36:04.03 AS.seek 0.66ml @13:36:14.28 delay 20 seconds @13:36:34.31 AS.reconfigure ASslow1 @13:36:34.37 AS.seek 0.705ml @13:36:43.67 delay 10 seconds @13:36:53.69 ATV.dial :air1 @13:36:55.43 AS.seek 0.725ml @13:36:59.73 delay 3 seconds @13:37:02.75 ATV.dial :waste @13:37:03.32 AS.reconfigure ASslow0 @13:37:03.37 AS.seek :empty @13:38:16.82 Setting up PCR for Pp -- writing /var/log/esp/Pp19may1013h.pcr @13:38:16.84 Building PCR Slug from extSrc with MM1 mix, PP2 primer @13:38:16.87 Priming with carrier @13:38:16.88 ATV.dial ARV @13:38:18.40 ARV.dial :carrier @13:38:18.76 AS.reconfigure ASnorm @13:38:18.82 AS.seek 0.499ml @13:38:44.37 AFV.select :carrierSolution @13:38:44.49 delay 2 seconds @13:38:46.51 AS.reconfigure ASconfig @13:38:46.58 AS.seek 1ml @13:39:15.65 delay 1 second @13:39:16.67 AFV.select :holdingCoil @13:39:16.78 delay 1 second @13:39:17.79 ATV.dial :waste @13:39:19.50 AS.reconfigure ASnorm @13:39:19.57 AS.seek :empty @13:40:10.26 AFV.select :carrierSolution @13:40:10.38 delay 2 seconds @13:40:12.40 AS.reconfigure ASconfig @13:40:12.47 AS.seek 0.5ml @13:40:41.56 delay 1 second @13:40:42.58 AFV.select :holdingCoil @13:40:42.69 Building Slug @13:40:42.70 ATV.dial :ARV @13:40:44.37 delay 2 seconds @13:40:46.39 ARV.dial :air1 @13:40:46.79 delay 2 seconds @13:40:48.86 AS.reconfigure ASslow2 @13:40:48.93 AS.seek 0.506ml @13:40:55.11 delay 2 seconds @13:40:57.13 ARV.dial :MM1 @13:40:57.83 delay 2 seconds @13:40:59.86 Adding MM1 @13:40:59.94 AS.seek 0.524ml @13:41:18.22 delay 25 seconds @13:41:43.24 ARV.dial :PP2 @13:41:43.64 Adding PP2 @13:41:43.66 delay 2 seconds @13:41:45.73 AS.seek 0.53ml @13:41:52.17 delay 10 seconds @13:42:02.20 ARV.dial :air2 @13:42:02.59 adding ARV air @13:42:02.61 delay 2 seconds @13:42:04.69 AS.seek 0.536ml @13:42:11.15 delay 5 seconds @13:42:16.17 ARV.dial :carrier @13:42:17.45 delay 7 seconds @13:42:24.52 AS.seek 0.594ml @13:43:23.04 delay 5 seconds @13:43:28.06 ATV.dial :extSrc @13:43:28.62 delay 2 seconds @13:43:30.69 AS.seek 0.6ml @13:43:36.87 delay 4 seconds @13:43:40.89 ATV.dial ARV @13:43:41.45 delay 2 seconds @13:43:43.47 ARV.dial :carrier @13:43:43.49 delay 2 seconds @13:43:45.57 AS.seek 0.65ml @13:44:36.04 delay 4 seconds @13:44:40.06 ATV.dial PCR @13:44:40.84 ASV.dial :waste1 @13:44:40.93 ARV.dial :air1 @13:44:41.44 Positioning Slug by 0.324ml @13:44:41.53 AS.seek 0.326ml @13:50:07.68 delay 5 seconds @13:50:12.70 ATV.dial ARV @13:50:13.50 ASV.dial :PCRlock @13:50:13.90 delay 5 seconds @13:50:18.93 Running Pp Profile60C350 thermocycling @13:50:18.94 PCR.program Profile60C350 @13:50:19.10 Profile60C350 should complete in < 1:48:05 @13:50:19.11 PCR.run 2:20:06.25 @14:07:57.24 Session Begun @14:12:27.34 -> showlog 100 @15:03:51.19 -> exit @15:03:51.21 Session Ended @15:14:01.84 PCR.off @15:14:03.83 PCR for Pp completed -- closed /var/log/esp/Pp19may1013h.pcr @15:14:03.90 Cleaning PCR @15:14:03.91 AFV.select :carrierSolution @15:14:04.04 delay 2 seconds @15:14:06.06 AS.reconfigure ASconfig @15:14:06.12 AS.seek 0.5ml @15:14:16.45 delay 1 second @15:14:17.47 AFV.select :holdingCoil @15:14:17.58 ASV.dial :waste1 @15:14:17.98 ATV.dial :air3 @15:14:19.29 AS.reconfigure ASslow1 @15:14:19.35 AS.seek 0.51ml @15:14:21.62 delay 2 seconds @15:14:23.64 ATV.dial :bleach @15:14:24.26 AS.seek 0.81ml @15:15:24.87 delay 3 seconds @15:15:27.89 ATV.dial :air3 @15:15:28.45 delay 3 seconds @15:15:31.52 AS.seek 0.835ml @15:15:36.81 delay 3 seconds @15:15:39.83 ATV.dial :bleach @15:15:40.45 AS.seek 0.82ml @15:15:43.76 delay 3 seconds @15:15:46.78 ARV.dial :waste @15:15:47.39 delay 3 seconds @15:15:50.41 ATV.dial :ARV @15:15:51.32 AS.reconfigure ASconfig @15:15:51.38 AS.seek 0.47ml @15:16:11.79 AFV.select :carrierSolution @15:16:11.91 delay 2 seconds @15:16:13.93 AS.seek 1ml @15:16:44.67 delay 1 second @15:16:45.69 AFV.select :holdingCoil @15:16:46.20 delay 1 second @15:16:47.22 AS.reconfigure ASfast @15:16:47.28 AS.seek 0ml @15:17:14.64 AFV.select :carrierSolution @15:17:14.76 delay 2 seconds @15:17:16.78 AS.reconfigure ASconfig @15:17:16.84 AS.seek 1ml @15:18:14.61 delay 1 second @15:18:15.63 AFV.select :holdingCoil @15:18:15.74 delay 1 second @15:18:16.76 AS.reconfigure ASfast @15:18:16.83 AS.seek 0ml @15:18:44.20 AFV.select :carrierSolution @15:18:44.32 delay 2 seconds @15:18:46.34 AS.reconfigure ASconfig @15:18:46.40 AS.seek 1ml @15:19:44.15 delay 1 second @15:19:45.17 AFV.select :holdingCoil @15:19:45.28 delay 1 second @15:19:46.30 AS.reconfigure ASfast @15:19:46.36 AS.seek 0ml @15:20:13.70 ARV.dial between carrier and air1 @15:20:14.20 ATV.dial :air3 @15:20:15.51 AS.reconfigure ASslow1 @15:20:15.57 AS.seek 0.01ml @15:20:17.95 delay 2 seconds @15:20:19.97 ATV.dial :bleach @15:20:20.59 AS.seek 0.81ml @15:23:01.86 delay 3 seconds @15:23:04.88 ATV.dial :air3 @15:23:05.43 delay 3 seconds @15:23:08.50 AS.seek 0.835ml @15:23:13.83 delay 3 seconds @15:23:16.85 ATV.dial :bleach @15:23:17.46 AS.seek 0.82ml @15:23:20.79 delay 3 seconds @15:23:23.81 ATV.dial PCR @15:23:24.23 AS.reconfigure ASconfig @15:23:24.29 AS.seek :empty @15:24:11.76 PCR.program :bleachProfile @15:24:11.82 PCR program should complete in < 2:45 @15:24:11.83 PCR.run 10:00 @15:27:07.60 PCR.off @15:27:07.64 AFV.select :carrierSolution @15:27:07.75 delay 2 seconds @15:27:09.77 AS.seek 1ml @15:28:07.61 delay 1 second @15:28:08.63 AFV.select :holdingCoil @15:28:08.74 delay 1 second @15:28:09.76 AS.reconfigure ASfast @15:28:09.81 AS.seek 0ml @15:28:37.28 delay 3 seconds @15:28:40.30 AFV.select :carrierSolution @15:28:40.41 delay 2 seconds @15:28:42.43 AS.reconfigure ASconfig @15:28:42.49 AS.seek 1ml @15:29:40.26 delay 1 second @15:29:41.28 AFV.select :holdingCoil @15:29:41.39 delay 1 second @15:29:42.41 AS.reconfigure ASfast @15:29:42.46 AS.seek 0ml @15:30:09.83 delay 3 seconds @15:30:12.85 AFV.select :carrierSolution @15:30:12.96 delay 2 seconds @15:30:14.98 AS.reconfigure ASconfig @15:30:15.04 AS.seek 1ml @15:31:12.79 delay 1 second @15:31:13.81 AFV.select :holdingCoil @15:31:13.92 delay 1 second @15:31:14.94 AS.reconfigure ASfast @15:31:15.00 AS.seek 0ml @15:31:42.36 delay 3 seconds @15:31:45.38 AFV.select :carrierSolution @15:31:45.49 delay 2 seconds @15:31:47.51 AS.reconfigure ASconfig @15:31:47.58 AS.seek 1ml @15:32:45.36 delay 1 second @15:32:46.38 AFV.select :holdingCoil @15:32:46.50 delay 1 second @15:32:47.52 AS.reconfigure ASfast @15:32:47.58 AS.seek 0ml @15:33:14.92 delay 3 seconds @15:33:17.94 AFV.select :carrierSolution @15:33:18.05 delay 2 seconds @15:33:20.07 AS.reconfigure ASconfig @15:33:20.13 AS.seek 1ml @15:34:17.89 delay 1 second @15:34:18.91 AFV.select :holdingCoil @15:34:19.03 delay 1 second @15:34:20.05 AS.reconfigure ASnorm @15:34:20.12 AS.seek 0ml @15:35:10.86
AFV.select :carrierSolution @15:35:10.97 delay 2 seconds @15:35:12.99 AS.reconfigure ASconfig @15:35:13.05 AS.seek 0.5ml @15:35:42.09 delay 1 second @15:35:43.11 AFV.select :holdingCoil @15:35:43.23 ATV.dial :air1 @15:35:44.74 AS.reconfigure ASslow2 @15:35:44.79 AS.seek 0.51ml @15:35:55.09 delay 5 seconds @15:36:00.11 ATV.dial :extSrc @15:36:01.85 AS.seek 0.542ml @15:36:34.20 delay 10 seconds @15:36:44.22 ATV.dial :air1 @15:36:45.96 AS.reconfigure ASslow1 @15:36:46.01 AS.seek 0.572ml @15:36:52.33 ATV.dial :waste @15:36:52.89 AS.reconfigure ASconfig @15:36:52.95 AS.seek :empty @15:37:26.37 Setting up PCR for Pp -- writing /var/log/esp/Pp19may1015h.pcr @15:37:26.39 Building PCR Slug from extSrc with MM1 mix, PP2 primer @15:37:26.42 Priming with carrier @15:37:26.44 ATV.dial ARV @15:37:27.97 ARV.dial :carrier @15:37:28.32 AS.reconfigure ASnorm @15:37:28.38 AS.seek 0.499ml @15:37:54.00 AFV.select :carrierSolution @15:37:54.12 delay 2 seconds @15:37:56.14 AS.reconfigure ASconfig @15:37:56.21 AS.seek 1ml @15:38:25.29 delay 1 second @15:38:26.31 AFV.select :holdingCoil @15:38:26.42 delay 1 second @15:38:27.43 ATV.dial :waste @15:38:29.15 AS.reconfigure ASnorm @15:38:29.21 AS.seek :empty @15:39:19.97 AFV.select :carrierSolution @15:39:20.09 delay 2 seconds @15:39:22.11 AS.reconfigure ASconfig @15:39:22.18 AS.seek 0.5ml @15:39:51.31 delay 1 second @15:39:52.33 AFV.select :holdingCoil @15:39:52.44 Building Slug @15:39:52.45 ATV.dial :ARV @15:39:54.12 delay 2 seconds @15:39:56.14 ARV.dial :air1 @15:39:56.55 delay 2 seconds @15:39:58.62 AS.reconfigure ASslow2 @15:39:59.09 AS.seek 0.506ml @15:40:05.28 delay 2 seconds @15:40:07.30 ARV.dial :MM1 @15:40:07.98 delay 2 seconds @15:40:10.01 Adding MM1 @15:40:10.09 AS.seek 0.524ml @15:40:28.59 delay 25 seconds @15:40:53.61 ARV.dial :PP2 @15:40:54.01 Adding PP2 @15:40:54.03 delay 2 seconds @15:40:56.11 AS.seek 0.53ml @15:41:02.36 delay 10 seconds @15:41:12.39 ARV.dial :air2 @15:41:12.78 adding ARV air @15:41:12.79 delay 2 seconds @15:41:14.88 AS.seek 0.536ml @15:41:21.17 delay 5 seconds @15:41:26.19 ARV.dial :carrier @15:41:27.47 delay 7 seconds @15:41:34.56 AS.seek 0.594ml @15:42:33.40 delay 5 seconds @15:42:38.42 ATV.dial :extSrc @15:42:38.98 delay 2 seconds @15:42:41.06 AS.seek 0.6ml @15:42:47.53 delay 4 seconds @15:42:51.55 ATV.dial ARV @15:42:52.11 delay 2 seconds @15:42:54.13 ARV.dial :carrier @15:42:54.15 delay 2 seconds @15:42:56.23 AS.seek 0.65ml @15:43:46.73 delay 4 seconds @15:43:50.75 ATV.dial PCR @15:43:51.53 ASV.dial :waste1 @15:43:51.54 ARV.dial :air1 @15:43:51.98 Positioning Slug by 0.324ml @15:43:52.07 AS.seek 0.326ml @15:49:18.25 delay 5 seconds @15:49:23.27 ATV.dial ARV @15:49:24.06 ASV.dial :PCRlock @15:49:24.47 delay 5 seconds @15:49:29.50 Running Pp Profile60C350 thermocycling @15:49:29.51 PCR.program Profile60C350 @15:49:29.66 Profile60C350 should complete in < 1:48:05 @15:49:29.67 PCR.run 2:20:06.25 @17:13:18.08 PCR.off @17:13:20.15 PCR for Pp completed -- closed /var/log/esp/Pp19may1015h.pcr @17:13:20.23 Cleaning PCR @17:13:20.24 AFV.select :carrierSolution @17:13:20.36 delay 2 seconds @17:13:22.38 AS.reconfigure ASconfig @17:13:22.44 AS.seek 0.5ml @17:13:32.76 delay 1 second @17:13:33.78 AFV.select :holdingCoil @17:13:33.89 ASV.dial :waste1 @17:13:34.29 ATV.dial :air3 @17:13:35.60 AS.reconfigure ASslow1 @17:13:35.66 AS.seek 0.51ml @17:13:37.96 delay 2 seconds @17:13:39.98 ATV.dial :bleach @17:13:40.60 AS.seek 0.81ml @17:14:41.20 delay 3 seconds @17:14:44.22 ATV.dial :air3 @17:14:44.78 delay 3 seconds @17:14:47.85 AS.seek 0.835ml @17:14:53.16 delay 3 seconds @17:14:56.18 ATV.dial :bleach @17:14:56.82 AS.seek 0.82ml @17:15:00.12 delay 3 seconds @17:15:03.14 ARV.dial :waste @17:15:03.76 delay 3 seconds @17:15:06.78 ATV.dial :ARV @17:15:07.79 AS.reconfigure ASconfig @17:15:07.84 AS.seek 0.47ml @17:15:28.26 AFV.select :carrierSolution @17:15:28.38 delay 2 seconds @17:15:30.40 AS.seek 1ml @17:16:01.17 delay 1 second @17:16:02.19 AFV.select :holdingCoil @17:16:02.31 delay 1 second @17:16:03.33 AS.reconfigure ASfast @17:16:03.39 AS.seek 0ml @17:16:30.75 AFV.select :carrierSolution @17:16:30.87 delay 2 seconds @17:16:32.89 AS.reconfigure ASconfig @17:16:32.94 AS.seek 1ml @17:17:30.70 delay 1 second @17:17:31.72 AFV.select :holdingCoil @17:17:31.83 delay 1 second @17:17:32.85 AS.reconfigure ASfast @17:17:32.91 AS.seek 0ml @17:18:00.26 AFV.select :carrierSolution @17:18:00.38 delay 2 seconds @17:18:02.40 AS.reconfigure ASconfig @17:18:02.45 AS.seek 1ml @17:19:00.25 delay 1 second @17:19:01.27 AFV.select :holdingCoil @17:19:01.39 delay 1 second @17:19:02.41 AS.reconfigure ASfast @17:19:02.47 AS.seek 0ml @17:19:29.84 ARV.dial between carrier and air1 @17:19:30.34 ATV.dial :air3 @17:19:31.65 AS.reconfigure ASslow1 @17:19:31.71 AS.seek 0.01ml @17:19:34.07 delay 2 seconds @17:19:36.09 ATV.dial :bleach @17:19:36.90 AS.seek 0.81ml @17:22:18.11 delay 3 seconds @17:22:21.13 ATV.dial :air3 @17:22:21.68 delay 3 seconds @17:22:24.75 AS.seek 0.835ml @17:22:30.10 delay 3 seconds @17:22:33.12 ATV.dial :bleach @17:22:33.74 AS.seek 0.82ml @17:22:37.04 delay 3 seconds @17:22:40.06 ATV.dial PCR @17:22:40.48 AS.reconfigure ASconfig @17:22:40.54 AS.seek :empty @17:23:28.03 PCR.program :bleachProfile @17:23:28.09 PCR program should complete in < 2:45 @17:23:28.10 PCR.run 10:00 @17:26:23.88 PCR.off @17:26:23.92 AFV.select :carrierSolution @17:26:24.03 delay 2 seconds @17:26:26.05 AS.seek 1ml @17:27:23.92 delay 1 second @17:27:24.94 AFV.select :holdingCoil @17:27:25.06 delay 1 second @17:27:26.08 AS.reconfigure ASfast @17:27:26.14 AS.seek 0ml @17:27:53.51 delay 3 seconds @17:27:56.53 AFV.select :carrierSolution @17:27:56.64 delay 2 seconds @17:27:58.66 AS.reconfigure ASconfig @17:27:58.71 AS.seek 1ml @17:28:56.47 delay 1 second @17:28:57.49 AFV.select :holdingCoil @17:28:57.61 delay 1 second @17:28:58.63 AS.reconfigure ASfast @17:28:58.68 AS.seek 0ml @17:29:26.03 delay 3 seconds @17:29:29.05 AFV.select :carrierSolution @17:29:29.16 delay 2 seconds @17:29:31.18 AS.reconfigure ASconfig @17:29:31.24 AS.seek 1ml @17:30:29.01 delay 1 second @17:30:30.03 AFV.select :holdingCoil @17:30:30.14 delay 1 second @17:30:31.16 AS.reconfigure ASfast @17:30:31.22 AS.seek 0ml @17:30:58.57 delay 3 seconds @17:31:01.59 AFV.select :carrierSolution @17:31:01.70 delay 2 seconds @17:31:03.72 AS.reconfigure ASconfig @17:31:03.77 AS.seek 1ml @17:32:01.53 delay 1 second @17:32:02.55 AFV.select :holdingCoil @17:32:02.67 delay 1 second @17:32:03.69 AS.reconfigure ASfast @17:32:03.74 AS.seek 0ml @17:32:31.11 delay 3 seconds @17:32:34.13 AFV.select :carrierSolution @17:32:34.25 delay 2 seconds @17:32:36.27 AS.reconfigure ASconfig @17:32:36.33 AS.seek 1ml @17:33:34.09 delay 1 second @17:33:35.11 AFV.select :holdingCoil @17:33:35.22 delay 1 second @17:33:36.24 AS.reconfigure ASnorm @17:33:36.30 AS.seek 0ml @17:34:27.03
AFV.select :carrierSolution @17:34:27.14 delay 2 seconds @17:34:29.16 AS.reconfigure ASconfig @17:34:29.21 AS.seek 0.5ml @17:34:58.30 delay 1 second @17:34:59.32 AFV.select :holdingCoil @17:34:59.44 ATV.dial :air1 @17:35:00.95 AS.reconfigure ASslow1 @17:35:01.01 AS.seek 0.52ml @17:35:05.29 delay 5 seconds @17:35:10.31 ATV.dial :extSrc @17:35:12.06 AS.reconfigure ASslow2 @17:35:12.12 AS.seek 0.557ml @17:35:49.45 delay 10 seconds @17:35:59.48 ATV.dial :air1 @17:36:01.23 AS.reconfigure ASslow1 @17:36:01.29 AS.seek 0.587ml @17:36:07.59 ATV.dial :waste @17:36:08.15 AS.reconfigure ASconfig @17:36:08.21 AS.seek :empty @17:36:42.44 Setting up PCR for Pp -- writing /var/log/esp/Pp19may1017h.pcr @17:36:42.47 Building PCR Slug from extSrc with MM1 mix, PP2 primer @17:36:42.48 Priming with carrier @17:36:42.51 ATV.dial ARV @17:36:44.03 ARV.dial :carrier @17:36:44.40 AS.reconfigure ASnorm @17:36:44.46 AS.seek 0.499ml @17:37:10.04 AFV.select :carrierSolution @17:37:10.16 delay 2 seconds @17:37:12.18 AS.reconfigure ASconfig @17:37:12.25 AS.seek 1ml @17:37:41.32 delay 1 second @17:37:42.34 AFV.select :holdingCoil @17:37:42.45 delay 1 second @17:37:43.46 ATV.dial :waste @17:37:45.16 AS.reconfigure ASnorm @17:37:45.22 AS.seek :empty @17:38:35.97 AFV.select :carrierSolution @17:38:36.10 delay 2 seconds @17:38:38.12 AS.reconfigure ASconfig @17:38:38.19 AS.seek 0.5ml @17:39:07.31 delay 1 second @17:39:08.33 AFV.select :holdingCoil @17:39:08.44 Building Slug @17:39:08.45 ATV.dial :ARV @17:39:10.12 delay 2 seconds @17:39:12.14 ARV.dial :air1 @17:39:12.55 delay 2 seconds @17:39:14.62 AS.reconfigure ASslow2 @17:39:14.69 AS.seek 0.506ml @17:39:20.90 delay 2 seconds @17:39:22.92 ARV.dial :MM1 @17:39:23.61 delay 2 seconds @17:39:25.64 Adding MM1 @17:39:25.72 AS.seek 0.524ml @17:39:43.97 delay 25 seconds @17:40:08.99 ARV.dial :PP2 @17:40:09.39 Adding PP2 @17:40:09.41 delay 2 seconds @17:40:11.49 AS.seek 0.53ml @17:40:17.76 delay 10 seconds @17:40:27.79 ARV.dial :air2 @17:40:28.17 adding ARV air @17:40:28.18 delay 2 seconds @17:40:30.27 AS.seek 0.536ml @17:40:36.54 delay 5 seconds @17:40:41.56 ARV.dial :carrier @17:40:42.84 delay 7 seconds @17:40:49.92 AS.seek 0.594ml @17:41:48.50 delay 5 seconds @17:41:53.52 ATV.dial :extSrc @17:41:54.09 delay 2 seconds @17:41:56.17 AS.seek 0.6ml @17:42:02.65 delay 4 seconds @17:42:06.67 ATV.dial ARV @17:42:07.23 delay 2 seconds @17:42:09.25 ARV.dial :carrier @17:42:09.27 delay 2 seconds @17:42:11.35 AS.seek 0.65ml @17:43:02.00 delay 4 seconds @17:43:06.02 ATV.dial PCR @17:43:06.79 ASV.dial :waste1 @17:43:06.80 ARV.dial :air1 @17:43:07.32 Positioning Slug by 0.324ml @17:43:07.41 AS.seek 0.326ml @17:48:33.51 delay 5 seconds @17:48:38.53 ATV.dial ARV @17:48:39.34 ASV.dial :PCRlock @17:48:39.75 delay 5 seconds @17:48:44.78 Running Pp Profile60C350 thermocycling @17:48:44.79 PCR.program Profile60C350 @17:48:44.94 Profile60C350 should complete in < 1:48:05 @17:48:44.95 PCR.run 2:20:06.25 @19:12:27.54 PCR.off @19:12:29.52 PCR for Pp completed -- closed /var/log/esp/Pp19may1017h.pcr @19:12:29.60 Cleaning PCR @19:12:29.61 AFV.select :carrierSolution @19:12:29.74 delay 2 seconds @19:12:31.76 AS.reconfigure ASconfig @19:12:31.81 AS.seek 0.5ml @19:12:42.12 delay 1 second @19:12:43.14 AFV.select :holdingCoil @19:12:43.24 ASV.dial :waste1 @19:12:43.64 ATV.dial :air3 @19:12:44.96 AS.reconfigure ASslow1 @19:12:45.02 AS.seek 0.51ml @19:12:47.33 delay 2 seconds @19:12:49.35 ATV.dial :bleach @19:12:49.99 AS.seek 0.81ml @19:13:50.59 delay 3 seconds @19:13:53.61 ATV.dial :air3 @19:13:54.17 delay 3 seconds @19:13:57.24 AS.seek 0.835ml @19:14:02.55 delay 3 seconds @19:14:05.57 ATV.dial :bleach @19:14:06.20 AS.seek 0.82ml @19:14:09.50 delay 3 seconds @19:14:12.52 ARV.dial :waste @19:14:13.12 delay 3 seconds @19:14:16.14 ATV.dial :ARV @19:14:17.06 AS.reconfigure ASconfig @19:14:17.12 AS.seek 0.47ml @19:14:37.93 AFV.select :carrierSolution @19:14:38.05 delay 2 seconds @19:14:40.07 AS.seek 1ml @19:15:10.81 delay 1 second @19:15:11.83 AFV.select :holdingCoil @19:15:11.94 delay 1 second @19:15:12.96 AS.reconfigure ASfast @19:15:13.02 AS.seek 0ml @19:15:40.37 AFV.select :carrierSolution @19:15:40.49 delay 2 seconds @19:15:42.51 AS.reconfigure ASconfig @19:15:42.57 AS.seek 1ml @19:16:40.31 delay 1 second @19:16:41.33 AFV.select :holdingCoil @19:16:41.44 delay 1 second @19:16:42.46 AS.reconfigure ASfast @19:16:42.53 AS.seek 0ml @19:17:09.89 AFV.select :carrierSolution @19:17:10.01 delay 2 seconds @19:17:12.03 AS.reconfigure ASconfig @19:17:12.09 AS.seek 1ml @19:18:09.84 delay 1 second @19:18:10.86 AFV.select :holdingCoil @19:18:10.97 delay 1 second @19:18:11.99 AS.reconfigure ASfast @19:18:12.05 AS.seek 0ml @19:18:39.40 ARV.dial between carrier and air1 @19:18:39.90 ATV.dial :air3 @19:18:41.21 AS.reconfigure ASslow1 @19:18:41.27 AS.seek 0.01ml @19:18:43.57 delay 2 seconds @19:18:45.59 ATV.dial :bleach @19:18:46.21 AS.seek 0.81ml @19:21:27.36 delay 3 seconds @19:21:30.38 ATV.dial :air3 @19:21:30.93 delay 3 seconds @19:21:34.00 AS.seek 0.835ml @19:21:39.32 delay 3 seconds @19:21:42.34 ATV.dial :bleach @19:21:42.96 AS.seek 0.82ml @19:21:46.26 delay 3 seconds @19:21:49.28 ATV.dial PCR @19:21:49.70 AS.reconfigure ASconfig @19:21:49.76 AS.seek :empty @19:22:37.25 PCR.program :bleachProfile @19:22:37.31 PCR program should complete in < 2:45 @19:22:37.32 PCR.run 10:00 @19:25:33.08 PCR.off @19:25:33.12 AFV.select :carrierSolution @19:25:33.24 delay 2 seconds @19:25:35.26 AS.seek 1ml @19:26:33.07 delay 1 second @19:26:34.09 AFV.select :holdingCoil @19:26:34.20 delay 1 second @19:26:35.22 AS.reconfigure ASfast @19:26:35.27 AS.seek 0ml @19:27:02.64 delay 3 seconds @19:27:05.66 AFV.select :carrierSolution @19:27:05.77 delay 2 seconds @19:27:07.79 AS.reconfigure ASconfig @19:27:07.85 AS.seek 1ml @19:28:05.61 delay 1 second @19:28:06.63 AFV.select :holdingCoil @19:28:06.74 delay 1 second @19:28:07.76 AS.reconfigure ASfast @19:28:07.81 AS.seek 0ml @19:28:35.26 delay 3 seconds @19:28:38.28 AFV.select :carrierSolution @19:28:38.39 delay 2 seconds @19:28:40.41 AS.reconfigure ASconfig @19:28:40.47 AS.seek 1ml @19:29:38.23 delay 1 second @19:29:39.25 AFV.select :holdingCoil @19:29:39.36 delay 1 second @19:29:40.38 AS.reconfigure ASfast @19:29:40.43 AS.seek 0ml @19:30:07.78 delay 3 seconds @19:30:10.80 AFV.select :carrierSolution @19:30:10.91 delay 2 seconds @19:30:12.93 AS.reconfigure ASconfig @19:30:13.00 AS.seek 1ml @19:31:10.84 delay 1 second @19:31:11.86 AFV.select :holdingCoil @19:31:11.97 delay 1 second @19:31:12.99 AS.reconfigure ASfast @19:31:13.04 AS.seek 0ml @19:31:40.42 delay 3 seconds @19:31:43.44 AFV.select :carrierSolution @19:31:43.55 delay 2 seconds @19:31:45.57 AS.reconfigure ASconfig @19:31:45.65 AS.seek 1ml @19:32:43.39 delay 1 second @19:32:44.41 AFV.select :holdingCoil @19:32:44.52 delay 1 second @19:32:45.54 AS.reconfigure ASnorm @19:32:45.59 AS.seek 0ml @19:33:36.30
AFV.select :carrierSolution @19:33:36.41 delay 2 seconds @19:33:38.43 AS.reconfigure ASconfig @19:33:38.49 AS.seek 0.5ml @19:34:07.54 delay 1 second @19:34:08.56 AFV.select :holdingCoil @19:34:08.68 ATV.dial :air1 @19:34:10.20 AS.reconfigure ASslow1 @19:34:10.25 AS.seek 0.52ml @19:34:14.54 delay 5 seconds @19:34:19.56 ATV.dial :extSrc @19:34:21.67 AS.reconfigure ASslow2 @19:34:21.73 AS.seek 0.538ml @19:34:40.03 delay 10 seconds @19:34:50.06 ATV.dial :air1 @19:34:51.81 AS.reconfigure ASslow1 @19:34:51.87 AS.seek 0.568ml @19:34:58.17 ATV.dial :waste @19:34:58.73 AS.reconfigure ASconfig @19:34:58.79 AS.seek :empty @19:35:31.81 deconEluateRecoveryLine extSrc @19:35:31.82 ATV.dial :waste @19:35:31.85 AS.reconfigure ASnorm @19:35:31.92 AS.seek 0ml @19:35:32.26 ATV.dial :air3 @19:35:32.96 AS.reconfigure ASslow1 @19:35:33.02 AS.seek 0.01ml @19:35:35.29 delay 2 seconds @19:35:37.31 ATV.dial :bleach @19:35:37.79 AS.seek 0.31ml @19:36:38.39 delay 3 seconds @19:36:41.41 ATV.dial :air3 @19:36:41.97 delay 3 seconds @19:36:45.04 AS.seek 0.335ml @19:36:50.35 delay 3 seconds @19:36:53.37 ATV.dial :bleach @19:36:53.99 AS.seek 0.32ml @19:36:57.29 delay 3 seconds @19:37:00.31 ATV.dial :extSrc @19:37:00.96 AS.seek 0ml @19:38:05.65 AFV.select :carrierSolution @19:38:05.77 delay 2 seconds @19:38:07.79 AS.reconfigure ASconfig @19:38:07.85 AS.seek 0.5ml @19:38:36.87 delay 1 second @19:38:37.89 AFV.select :holdingCoil @19:38:37.99 ATV.dial :extSrc @19:38:38.01 AS.reconfigure ASslow1 @19:38:38.09 AS.seek 0ml @19:40:18.98 AFV.select :carrierSolution @19:40:19.10 delay 2 seconds @19:40:21.12 AS.reconfigure ASconfig @19:40:21.19 AS.seek 0.5ml @19:40:50.22 delay 1 second @19:40:51.24 AFV.select :holdingCoil @19:40:51.34 ATV.dial :extSrc @19:40:51.36 AS.reconfigure ASslow1 @19:40:51.44 AS.seek 0ml @19:42:32.26 AFV.select :carrierSolution @19:42:32.38 delay 2 seconds @19:42:34.40 AS.reconfigure ASconfig @19:42:34.46 AS.seek 0.5ml @19:43:03.50 delay 1 second @19:43:04.52 AFV.select :holdingCoil @19:43:04.62 ATV.dial :extSrc @19:43:04.64 AS.reconfigure ASslow1 @19:43:04.72 AS.seek 0ml @19:44:45.54 AFV.select :carrierSolution @19:44:45.66 delay 2 seconds @19:44:47.68 AS.reconfigure ASconfig @19:44:47.74 AS.seek 0.5ml @19:45:16.78 delay 1 second @19:45:17.80 AFV.select :holdingCoil @19:45:17.90 ATV.dial :extSrc @19:45:17.92 AS.reconfigure ASslow1 @19:45:18.00 AS.seek 0ml @19:46:58.89 ATV.dial :air2 @19:47:00.24 AS.reconfigure ASslow0 @19:47:00.29 AS.seek 0.5ml @19:47:50.90 ATV.dial :extSrc @19:47:52.42 AS.seek 0.02ml @19:48:41.01 delay 1 second @19:48:42.03 ATV.dial :waste @19:48:43.36 AS.reconfigure ASconfig @19:48:43.42 AS.seek :empty @19:48:44.93 deconEluateRecoveryLine extSrc @19:48:44.94 ATV.dial :waste @19:48:44.97 AS.reconfigure ASnorm @19:48:45.04 AS.seek 0ml @19:48:45.42 ATV.dial :air3 @19:48:46.29 AS.reconfigure ASslow1 @19:48:46.35 AS.seek 0.01ml @19:48:48.64 delay 2 seconds @19:48:50.66 ATV.dial :bleach @19:48:51.14 AS.seek 0.31ml @19:49:51.75 delay 3 seconds @19:49:54.77 ATV.dial :air3 @19:49:55.34 delay 3 seconds @19:49:58.41 AS.seek 0.335ml @19:50:03.70 delay 3 seconds @19:50:06.72 ATV.dial :bleach @19:50:07.35 AS.seek 0.32ml @19:50:10.67 delay 3 seconds @19:50:13.69 ATV.dial :extSrc @19:50:14.32 AS.seek 0ml @19:51:18.92 AFV.select :carrierSolution @19:51:19.04 delay 2 seconds @19:51:21.06 AS.reconfigure ASconfig @19:51:21.11 AS.seek 0.5ml @19:51:50.14 delay 1 second @19:51:51.16 AFV.select :holdingCoil @19:51:51.26 ATV.dial :extSrc @19:51:51.28 AS.reconfigure ASslow1 @19:51:51.36 AS.seek 0ml @19:53:32.18 AFV.select :carrierSolution @19:53:32.30 delay 2 seconds @19:53:34.32 AS.reconfigure ASconfig @19:53:34.37 AS.seek 0.5ml @19:54:03.76 delay 1 second @19:54:04.78 AFV.select :holdingCoil @19:54:04.88 ATV.dial :extSrc @19:54:04.90 AS.reconfigure ASslow1 @19:54:04.98 AS.seek 0ml @19:55:45.81 AFV.select :carrierSolution @19:55:45.93 delay 2 seconds @19:55:47.95 AS.reconfigure ASconfig @19:55:48.01 AS.seek 0.5ml @19:56:17.04 delay 1 second @19:56:18.06 AFV.select :holdingCoil @19:56:18.16 ATV.dial :extSrc @19:56:18.18 AS.reconfigure ASslow1 @19:56:18.26 AS.seek 0ml @19:57:59.07 AFV.select :carrierSolution @19:57:59.19 delay 2 seconds @19:58:01.21 AS.reconfigure ASconfig @19:58:01.27 AS.seek 0.5ml @19:58:30.29 delay 1 second @19:58:31.31 AFV.select :holdingCoil @19:58:31.41 ATV.dial :extSrc @19:58:31.43 AS.reconfigure ASslow1 @19:58:31.51 AS.seek 0ml @20:00:12.37 ATV.dial :air2 @20:00:13.73 AS.reconfigure ASslow0 @20:00:13.79 AS.seek 0.5ml @20:01:04.34 ATV.dial :extSrc @20:01:05.85 AS.seek 0.02ml @20:01:54.43 delay 1 second @20:01:55.45 ATV.dial :waste @20:01:56.76 AS.reconfigure ASconfig @20:01:56.82 AS.seek :empty @20:01:58.33 deconEluateRecoveryLine extSrc @20:01:58.34 ATV.dial :waste @20:01:58.38 AS.reconfigure ASnorm @20:01:58.44 AS.seek 0ml @20:01:58.78 ATV.dial :air3 @20:01:59.65 AS.reconfigure ASslow1 @20:01:59.71 AS.seek 0.01ml @20:02:02.01 delay 2 seconds @20:02:04.03 ATV.dial :bleach @20:02:04.49 AS.seek 0.31ml @20:03:05.06 delay 3 seconds @20:03:08.08 ATV.dial :air3 @20:03:08.64 delay 3 seconds @20:03:11.71 AS.seek 0.335ml @20:03:17.04 delay 3 seconds @20:03:20.06 ATV.dial :bleach @20:03:20.70 AS.seek 0.32ml @20:03:24.03 delay 3 seconds @20:03:27.05 ATV.dial :extSrc @20:03:27.68 AS.seek 0ml @20:04:32.28 AFV.select :carrierSolution @20:04:32.40 delay 2 seconds @20:04:34.42 AS.reconfigure ASconfig @20:04:34.48 AS.seek 0.5ml @20:05:03.51 delay 1 second @20:05:04.53 AFV.select :holdingCoil @20:05:04.63 ATV.dial :extSrc @20:05:04.65 AS.reconfigure ASslow1 @20:05:04.73 AS.seek 0ml @20:06:45.61 AFV.select :carrierSolution @20:06:45.73 delay 2 seconds @20:06:47.75 AS.reconfigure ASconfig @20:06:47.81 AS.seek 0.5ml @20:07:16.89 delay 1 second @20:07:17.91 AFV.select :holdingCoil @20:07:18.01 ATV.dial :extSrc @20:07:18.03 AS.reconfigure ASslow1 @20:07:18.11 AS.seek 0ml @20:08:58.93 AFV.select :carrierSolution @20:08:59.05 delay 2 seconds @20:09:01.07 AS.reconfigure ASconfig @20:09:01.12 AS.seek 0.5ml @20:09:30.17 delay 1 second @20:09:31.19 AFV.select :holdingCoil @20:09:31.29 ATV.dial :extSrc @20:09:31.31 AS.reconfigure ASslow1 @20:09:31.39 AS.seek 0ml @20:11:12.20 AFV.select :carrierSolution @20:11:12.32 delay 2 seconds @20:11:14.34 AS.reconfigure ASconfig @20:11:14.39 AS.seek 0.5ml @20:11:43.43 delay 1 second @20:11:44.45 AFV.select :holdingCoil @20:11:44.55 ATV.dial :extSrc @20:11:44.57 AS.reconfigure ASslow1 @20:11:44.65 AS.seek 0ml @20:13:25.48 ATV.dial :air2 @20:13:26.85 AS.reconfigure ASslow0 @20:13:26.90 AS.seek 0.5ml @20:14:17.53 ATV.dial :extSrc @20:14:19.06 AS.seek 0.02ml @20:15:07.67 delay 1 second @20:15:08.69 ATV.dial :waste @20:15:10.00 AS.reconfigure ASconfig @20:15:10.06 AS.seek :empty @20:15:11.63 Session Ended @16:45:46.80CEST13-May-19
ESP2 v3.30 I2C Gateway v3.06 for core bus @16:45:48.45 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @16:45:48.71 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @16:45:52.90 Polling Can every 10:00 @16:45:54.65 SPE.coast @16:45:54.70 SPE.configure SPEconfig @16:45:54.78 AS.reconfigure ASconfig @16:45:54.82 AS.hold @16:45:54.86 AS.configure ASconfig @16:45:54.95 SS.configure SSconfig @16:45:55.03 CC.configure CCconfig @16:45:55.11 CS.reconfigure CSconfig @16:45:55.14 CS.hold @16:45:55.21 CS.configure CSconfig @16:45:55.29 PC.configure PCconfig @16:45:55.37 PS.reconfigure PSconfig @16:45:55.40 PS.hold @16:45:55.49 PS.configure PSconfig @16:45:55.87 I2C::NodeOffline in MAIN -- manipulator Not Responding MISSING Hand @16:45:55.89 I2C::NodeOffline in MAIN -- manipulator Not Responding @16:45:55.90 MISSING Forearm @16:45:55.93 I2C::NodeOffline in MAIN -- manipulator Not Responding MISSING Elbow @16:45:56.00 SC.configure SCloose @16:45:56.08 SE.configure SEconfig @16:45:56.12 CH.coast @16:45:56.17 CH.configure CHconfig @16:45:56.19 PH.coast @16:45:56.24 PH.configure PHconfig @16:45:56.28 PCR.off @16:45:56.31 PCR.pidtemp= 50,0.1,0,300,25,25 @16:45:56.33 PCR.caltemp= 2.8814,0.0372,0 @16:45:56.36 All dwarf controllers running firmware version 3.75 @16:45:56.52 Cleanly exited at 16:44:30CEST13-May-19 @16:45:56.55 Loading /home/esp/esp2/mission/stdMERwithoutESPready2.rb mission @16:45:56.57 AFV.select :carrierSolution @16:45:56.67 delay 2 seconds @16:45:58.70 AS.seek 0.5ml @16:46:23.60 Session Begun @16:46:27.75
delay 1 second @16:46:28.77 AFV.select :holdingCoil @16:46:28.87 ATV.dial :extSrc @16:46:30.38 AS.reconfigure ASslow1 @16:46:30.44 AS.seek 0.53ml @16:46:36.75 delay 5 seconds @16:46:41.83 AS.reconfigure ASslow2 @16:46:41.89 AS.seek 0.54ml @16:46:52.20 delay 20 seconds @16:47:12.27 AS.reconfigure ASslow1 @16:47:12.33 AS.seek 0.57ml @16:47:18.70 delay 5 seconds @16:47:23.78 AS.reconfigure ASslow2 @16:47:23.84 AS.seek 0.58ml @16:47:34.31 delay 20 seconds @16:47:54.39 AS.reconfigure ASslow1 @16:47:54.45 AS.seek 0.61ml @16:48:00.73 delay 5 seconds @16:48:05.81 AS.reconfigure ASslow2 @16:48:05.87 AS.seek 0.62ml @16:48:16.07 delay 20 seconds @16:48:36.14 AS.reconfigure ASslow1 @16:48:36.20 AS.seek 0.65ml @16:48:42.50 delay 5 seconds @16:48:47.58 AS.reconfigure ASslow2 @16:48:47.64 AS.seek 0.66ml @16:48:58.06 delay 20 seconds @16:49:18.09 AS.reconfigure ASslow1 @16:49:18.14 AS.seek 0.705ml @16:49:27.48 delay 10 seconds @16:49:37.50 ATV.dial :air1 @16:49:39.26 AS.seek 0.725ml @16:49:43.50 delay 3 seconds @16:49:46.52 ATV.dial :waste @16:49:47.09 AS.reconfigure ASslow0 @16:49:47.15 AS.seek :empty @16:51:00.60 Setting up PCR for Mm -- writing /var/log/esp/Mm19may1316h.pcr @16:51:00.62 Building PCR Slug from extSrc with MM2 mix, PP3 primer @16:51:00.65 Priming with carrier @16:51:00.66 ATV.dial ARV @16:51:02.20 ARV.dial :carrier @16:51:02.56 AS.reconfigure ASnorm @16:51:02.62 AS.seek 0.499ml @16:51:28.21 AFV.select :carrierSolution @16:51:28.32 delay 2 seconds @16:51:30.35 AS.reconfigure ASconfig @16:51:30.41 AS.seek 1ml @16:51:59.50 delay 1 second @16:52:00.52 AFV.select :holdingCoil @16:52:00.62 delay 1 second @16:52:01.64 ATV.dial :waste @16:52:03.36 AS.reconfigure ASnorm @16:52:03.42 AS.seek :empty @16:52:54.20 AFV.select :carrierSolution @16:52:54.31 delay 2 seconds @16:52:56.34 AS.reconfigure ASconfig @16:52:56.39 AS.seek 0.5ml @16:53:25.53 delay 1 second @16:53:26.55 AFV.select :holdingCoil @16:53:26.66 Building Slug @16:53:26.67 ATV.dial :ARV @16:53:28.45 delay 2 seconds @16:53:30.47 ARV.dial :air1 @16:53:30.88 delay 2 seconds @16:53:32.95 AS.reconfigure ASslow2 @16:53:33.01 AS.seek 0.506ml @16:53:39.65 delay 2 seconds @16:53:41.67 ARV.dial :MM2 @16:53:43.15 delay 2 seconds @16:53:45.17 Adding MM2 @16:53:45.26 AS.seek 0.524ml @16:54:03.68 delay 25 seconds @16:54:28.70 ARV.dial :PP3 @16:54:29.07 Adding PP3 @16:54:29.08 delay 2 seconds @16:54:31.16 AS.seek 0.53ml @16:54:37.45 delay 10 seconds @16:54:47.48 ARV.dial :air2 @16:54:47.89 adding ARV air @16:54:47.90 delay 2 seconds @16:54:49.98 AS.seek 0.536ml @16:54:56.17 delay 5 seconds @16:55:01.19 ARV.dial :carrier @16:55:02.48 delay 7 seconds @16:55:09.56 AS.seek 0.594ml @16:56:08.20 delay 5 seconds @16:56:13.22 ATV.dial :extSrc @16:56:13.79 delay 2 seconds @16:56:15.86 AS.seek 0.6ml @16:56:22.36 delay 4 seconds @16:56:26.38 ATV.dial ARV @16:56:26.95 delay 2 seconds @16:56:28.97 ARV.dial :carrier @16:56:28.98 delay 2 seconds @16:56:31.06 AS.seek 0.65ml @16:57:21.70 delay 4 seconds @16:57:25.72 ATV.dial PCR @16:57:26.50 ASV.dial :waste1 @16:57:26.59 ARV.dial :air1 @16:57:27.01 Positioning Slug by 0.324ml @16:57:27.11 AS.seek 0.326ml @17:02:53.53 delay 5 seconds @17:02:58.55 ATV.dial ARV @17:02:59.34 ASV.dial :PCRlock @17:02:59.75 delay 5 seconds @17:03:04.77 Running Mm Profile60C350 thermocycling @17:03:04.79 PCR.program Profile60C350 @17:03:04.94 Profile60C350 should complete in < 1:48:05 @17:03:04.95 PCR.run 2:20:06.25 @18:26:33.19 PCR.off @18:26:35.17 PCR for Mm completed -- closed /var/log/esp/Mm19may1316h.pcr @18:26:35.25 Cleaning PCR @18:26:35.26 AFV.select :carrierSolution @18:26:35.37 delay 2 seconds @18:26:37.40 AS.reconfigure ASconfig @18:26:37.45 AS.seek 0.5ml @18:26:47.74 delay 1 second @18:26:48.76 AFV.select :holdingCoil @18:26:48.86 ASV.dial :waste1 @18:26:49.26 ATV.dial :air3 @18:26:50.59 AS.reconfigure ASslow1 @18:26:50.65 AS.seek 0.51ml @18:26:52.94 delay 2 seconds @18:26:54.96 ATV.dial :bleach @18:26:55.60 AS.seek 0.81ml @18:27:56.18 delay 3 seconds @18:27:59.20 ATV.dial :air3 @18:27:59.76 delay 3 seconds @18:28:02.83 AS.seek 0.835ml @18:28:08.15 delay 3 seconds @18:28:11.17 ATV.dial :bleach @18:28:11.81 AS.seek 0.82ml @18:28:15.15 delay 3 seconds @18:28:18.17 ARV.dial :waste @18:28:18.79 delay 3 seconds @18:28:21.81 ATV.dial :ARV @18:28:22.73 AS.reconfigure ASconfig @18:28:22.79 AS.seek 0.47ml @18:28:43.20 AFV.select :carrierSolution @18:28:43.31 delay 2 seconds @18:28:45.34 AS.seek 1ml @18:29:16.14 delay 1 second @18:29:17.16 AFV.select :holdingCoil @18:29:17.26 delay 1 second @18:29:18.29 AS.reconfigure ASfast @18:29:18.34 AS.seek 0ml @18:29:46.09 AFV.select :carrierSolution @18:29:46.20 delay 2 seconds @18:29:48.23 AS.reconfigure ASconfig @18:29:48.28 AS.seek 1ml @18:30:46.03 delay 1 second @18:30:47.05 AFV.select :holdingCoil @18:30:47.15 delay 1 second @18:30:48.18 AS.reconfigure ASfast @18:30:48.23 AS.seek 0ml @18:31:15.59 AFV.select :carrierSolution @18:31:15.70 delay 2 seconds @18:31:17.73 AS.reconfigure ASconfig @18:31:17.78 AS.seek 1ml @18:32:15.59 delay 1 second @18:32:16.61 AFV.select :holdingCoil @18:32:16.71 delay 1 second @18:32:17.74 AS.reconfigure ASfast @18:32:17.79 AS.seek 0ml @18:32:45.15 ARV.dial between carrier and air1 @18:32:45.65 ATV.dial :air3 @18:32:46.96 AS.reconfigure ASslow1 @18:32:47.02 AS.seek 0.01ml @18:32:49.32 delay 2 seconds @18:32:51.34 ATV.dial :bleach @18:32:51.96 AS.seek 0.81ml @18:35:33.09 delay 3 seconds @18:35:36.11 ATV.dial :air3 @18:35:36.67 delay 3 seconds @18:35:39.74 AS.seek 0.835ml @18:35:45.04 delay 3 seconds @18:35:48.06 ATV.dial :bleach @18:35:48.68 AS.seek 0.82ml @18:35:51.99 delay 3 seconds @18:35:55.01 ATV.dial PCR @18:35:55.43 AS.reconfigure ASconfig @18:35:55.49 AS.seek :empty @18:36:42.98 PCR.program :bleachProfile @18:36:43.03 PCR program should complete in < 2:45 @18:36:43.04 PCR.run 10:00 @18:39:38.81 PCR.off @18:39:38.84 AFV.select :carrierSolution @18:39:38.95 delay 2 seconds @18:39:40.98 AS.seek 1ml @18:40:38.81 delay 1 second @18:40:39.83 AFV.select :holdingCoil @18:40:39.93 delay 1 second @18:40:40.96 AS.reconfigure ASfast @18:40:41.01 AS.seek 0ml @18:41:08.37 delay 3 seconds @18:41:11.39 AFV.select :carrierSolution @18:41:11.49 delay 2 seconds @18:41:13.52 AS.reconfigure ASconfig @18:41:13.59 AS.seek 1ml @18:42:11.39 delay 1 second @18:42:12.41 AFV.select :holdingCoil @18:42:12.51 delay 1 second @18:42:13.54 AS.reconfigure ASfast @18:42:13.59 AS.seek 0ml @18:42:40.95 delay 3 seconds @18:42:43.97 AFV.select :carrierSolution @18:42:44.07 delay 2 seconds @18:42:46.10 AS.reconfigure ASconfig @18:42:46.16 AS.seek 1ml @18:43:43.92 delay 1 second @18:43:44.94 AFV.select :holdingCoil @18:43:45.04 delay 1 second @18:43:46.07 AS.reconfigure ASfast @18:43:46.12 AS.seek 0ml @18:44:13.48 delay 3 seconds @18:44:16.50 AFV.select :carrierSolution @18:44:16.60 delay 2 seconds @18:44:18.63 AS.reconfigure ASconfig @18:44:18.69 AS.seek 1ml @18:45:16.46 delay 1 second @18:45:17.48 AFV.select :holdingCoil @18:45:17.58 delay 1 second @18:45:18.61 AS.reconfigure ASfast @18:45:18.66 AS.seek 0ml @18:45:46.01 delay 3 seconds @18:45:49.03 AFV.select :carrierSolution @18:45:49.13 delay 2 seconds @18:45:51.16 AS.reconfigure ASconfig @18:45:51.22 AS.seek 1ml @18:46:48.99 delay 1 second @18:46:50.01 AFV.select :holdingCoil @18:46:50.12 delay 1 second @18:46:51.15 AS.reconfigure ASnorm @18:46:51.20 AS.seek 0ml @18:47:41.91
AFV.select :carrierSolution @18:47:42.02 delay 2 seconds @18:47:44.05 AS.reconfigure ASconfig @18:47:44.10 AS.seek 0.5ml @18:48:13.17 delay 1 second @18:48:14.19 AFV.select :holdingCoil @18:48:14.30 ATV.dial :air1 @18:48:15.81 AS.reconfigure ASslow2 @18:48:15.87 AS.seek 0.51ml @18:48:26.15 delay 5 seconds @18:48:31.17 ATV.dial :extSrc @18:48:32.90 AS.seek 0.542ml @18:49:05.28 delay 10 seconds @18:49:15.30 ATV.dial :air1 @18:49:17.03 AS.reconfigure ASslow1 @18:49:17.09 AS.seek 0.572ml @18:49:23.40 ATV.dial :waste @18:49:23.96 AS.reconfigure ASconfig @18:49:24.02 AS.seek :empty @18:49:57.40 Setting up PCR for Mm -- writing /var/log/esp/Mm19may1318h.pcr @18:49:57.43 Building PCR Slug from extSrc with MM2 mix, PP3 primer @18:49:57.46 Priming with carrier @18:49:57.48 ATV.dial ARV @18:49:59.01 ARV.dial :carrier @18:49:59.35 AS.reconfigure ASnorm @18:49:59.41 AS.seek 0.499ml @18:50:24.98 AFV.select :carrierSolution @18:50:25.09 delay 2 seconds @18:50:27.12 AS.reconfigure ASconfig @18:50:27.18 AS.seek 1ml @18:50:56.26 delay 1 second @18:50:57.28 AFV.select :holdingCoil @18:50:57.38 delay 1 second @18:50:58.40 ATV.dial :waste @18:51:00.12 AS.reconfigure ASnorm @18:51:00.18 AS.seek :empty @18:51:50.93 AFV.select :carrierSolution @18:51:51.05 delay 2 seconds @18:51:53.08 AS.reconfigure ASconfig @18:51:53.13 AS.seek 0.5ml @18:52:22.23 delay 1 second @18:52:23.25 AFV.select :holdingCoil @18:52:23.36 Building Slug @18:52:23.37 ATV.dial :ARV @18:52:25.04 delay 2 seconds @18:52:27.06 ARV.dial :air1 @18:52:27.46 delay 2 seconds @18:52:29.53 AS.reconfigure ASslow2 @18:52:29.59 AS.seek 0.506ml @18:52:36.24 delay 2 seconds @18:52:38.32 ARV.dial :MM2 @18:52:39.79 delay 2 seconds @18:52:41.81 Adding MM2 @18:52:41.90 AS.seek 0.524ml @18:53:00.34 delay 25 seconds @18:53:25.36 ARV.dial :PP3 @18:53:25.74 Adding PP3 @18:53:25.75 delay 2 seconds @18:53:27.83 AS.seek 0.53ml @18:53:34.04 delay 10 seconds @18:53:44.07 ARV.dial :air2 @18:53:44.46 adding ARV air @18:53:44.47 delay 2 seconds @18:53:46.55 AS.seek 0.536ml @18:53:52.93 delay 5 seconds @18:53:57.95 ARV.dial :carrier @18:53:59.23 delay 7 seconds @18:54:06.31 AS.seek 0.594ml @18:55:04.79 delay 5 seconds @18:55:09.81 ATV.dial :extSrc @18:55:10.37 delay 2 seconds @18:55:12.44 AS.seek 0.6ml @18:55:18.68 delay 4 seconds @18:55:22.70 ATV.dial ARV @18:55:23.26 delay 2 seconds @18:55:25.28 ARV.dial :carrier @18:55:25.29 delay 2 seconds @18:55:27.37 AS.seek 0.65ml @18:56:18.06 delay 4 seconds @18:56:22.08 ATV.dial PCR @18:56:22.87 ASV.dial :waste1 @18:56:22.88 ARV.dial :air1 @18:56:23.31 Positioning Slug by 0.324ml @18:56:23.40 AS.seek 0.326ml @19:01:49.43 delay 5 seconds @19:01:54.45 ATV.dial ARV @19:01:55.28 ASV.dial :PCRlock @19:01:55.68 delay 5 seconds @19:02:00.70 Running Mm Profile60C350 thermocycling @19:02:00.72 PCR.program Profile60C350 @19:02:00.86 Profile60C350 should complete in < 1:48:05 @19:02:00.87 PCR.run 2:20:06.25 @20:25:38.07 PCR.off @20:25:40.22 PCR for Mm completed -- closed /var/log/esp/Mm19may1318h.pcr @20:25:40.30 Cleaning PCR @20:25:40.31 AFV.select :carrierSolution @20:25:40.42 delay 2 seconds @20:25:42.45 AS.reconfigure ASconfig @20:25:42.50 AS.seek 0.5ml @20:25:52.82 delay 1 second @20:25:53.84 AFV.select :holdingCoil @20:25:53.95 ASV.dial :waste1 @20:25:54.35 ATV.dial :air3 @20:25:55.66 AS.reconfigure ASslow1 @20:25:55.72 AS.seek 0.51ml @20:25:58.02 delay 2 seconds @20:26:00.04 ATV.dial :bleach @20:26:00.66 AS.seek 0.81ml @20:27:01.24 delay 3 seconds @20:27:04.26 ATV.dial :air3 @20:27:04.82 delay 3 seconds @20:27:07.89 AS.seek 0.835ml @20:27:13.21 delay 3 seconds @20:27:16.23 ATV.dial :bleach @20:27:16.87 AS.seek 0.82ml @20:27:20.18 delay 3 seconds @20:27:23.20 ARV.dial :waste @20:27:23.82 delay 3 seconds @20:27:26.84 ATV.dial :ARV @20:27:27.76 AS.reconfigure ASconfig @20:27:27.82 AS.seek 0.47ml @20:27:48.26 AFV.select :carrierSolution @20:27:48.37 delay 2 seconds @20:27:50.40 AS.seek 1ml @20:28:21.15 delay 1 second @20:28:22.17 AFV.select :holdingCoil @20:28:22.27 delay 1 second @20:28:23.30 AS.reconfigure ASfast @20:28:23.35 AS.seek 0ml @20:28:50.68 AFV.select :carrierSolution @20:28:50.79 delay 2 seconds @20:28:52.82 AS.reconfigure ASconfig @20:28:52.87 AS.seek 1ml @20:29:50.63 delay 1 second @20:29:51.65 AFV.select :holdingCoil @20:29:51.75 delay 1 second @20:29:52.78 AS.reconfigure ASfast @20:29:52.83 AS.seek 0ml @20:30:20.20 AFV.select :carrierSolution @20:30:20.31 delay 2 seconds @20:30:22.34 AS.reconfigure ASconfig @20:30:22.39 AS.seek 1ml @20:31:20.15 delay 1 second @20:31:21.17 AFV.select :holdingCoil @20:31:21.27 delay 1 second @20:31:22.30 AS.reconfigure ASfast @20:31:22.35 AS.seek 0ml @20:31:49.70 ARV.dial between carrier and air1 @20:31:50.20 ATV.dial :air3 @20:31:51.52 AS.reconfigure ASslow1 @20:31:51.58 AS.seek 0.01ml @20:31:53.87 delay 2 seconds @20:31:55.89 ATV.dial :bleach @20:31:56.51 AS.seek 0.81ml @20:34:37.74 delay 3 seconds @20:34:40.76 ATV.dial :air3 @20:34:41.32 delay 3 seconds @20:34:44.39 AS.seek 0.835ml @20:34:49.72 delay 3 seconds @20:34:52.74 ATV.dial :bleach @20:34:53.37 AS.seek 0.82ml @20:34:56.79 delay 3 seconds @20:34:59.81 ATV.dial PCR @20:35:00.23 AS.reconfigure ASconfig @20:35:00.29 AS.seek :empty @20:35:47.78 PCR.program :bleachProfile @20:35:47.84 PCR program should complete in < 2:45 @20:35:47.85 PCR.run 10:00 @20:38:43.62 PCR.off @20:38:43.65 AFV.select :carrierSolution @20:38:43.77 delay 2 seconds @20:38:45.80 AS.seek 1ml @20:39:43.63 delay 1 second @20:39:44.65 AFV.select :holdingCoil @20:39:44.75 delay 1 second @20:39:45.78 AS.reconfigure ASfast @20:39:45.83 AS.seek 0ml @20:40:13.21 delay 3 seconds @20:40:16.23 AFV.select :carrierSolution @20:40:16.33 delay 2 seconds @20:40:18.36 AS.reconfigure ASconfig @20:40:18.42 AS.seek 1ml @20:41:16.18 delay 1 second @20:41:17.20 AFV.select :holdingCoil @20:41:17.30 delay 1 second @20:41:18.33 AS.reconfigure ASfast @20:41:18.38 AS.seek 0ml @20:41:45.74 delay 3 seconds @20:41:48.76 AFV.select :carrierSolution @20:41:48.86 delay 2 seconds @20:41:50.89 AS.reconfigure ASconfig @20:41:50.95 AS.seek 1ml @20:42:48.71 delay 1 second @20:42:49.73 AFV.select :holdingCoil @20:42:49.83 delay 1 second @20:42:50.86 AS.reconfigure ASfast @20:42:50.91 AS.seek 0ml @20:43:18.26 delay 3 seconds @20:43:21.28 AFV.select :carrierSolution @20:43:21.38 delay 2 seconds @20:43:23.41 AS.reconfigure ASconfig @20:43:23.48 AS.seek 1ml @20:44:21.32 delay 1 second @20:44:22.34 AFV.select :holdingCoil @20:44:22.44 delay 1 second @20:44:23.47 AS.reconfigure ASfast @20:44:23.52 AS.seek 0ml @20:44:50.88 delay 3 seconds @20:44:53.90 AFV.select :carrierSolution @20:44:54.00 delay 2 seconds @20:44:56.03 AS.reconfigure ASconfig @20:44:56.09 AS.seek 1ml @20:45:53.88 delay 1 second @20:45:54.90 AFV.select :holdingCoil @20:45:55.01 delay 1 second @20:45:56.04 AS.reconfigure ASnorm @20:45:56.09 AS.seek 0ml @20:46:46.83
AFV.select :carrierSolution @20:46:46.94 delay 2 seconds @20:46:48.97 AS.reconfigure ASconfig @20:46:49.02 AS.seek 0.5ml @20:47:18.46 delay 1 second @20:47:19.48 AFV.select :holdingCoil @20:47:19.58 ATV.dial :air1 @20:47:21.10 AS.reconfigure ASslow1 @20:47:21.16 AS.seek 0.52ml @20:47:25.51 delay 5 seconds @20:47:30.53 ATV.dial :extSrc @20:47:32.27 AS.reconfigure ASslow2 @20:47:32.33 AS.seek 0.557ml @20:48:09.95 delay 10 seconds @20:48:19.98 ATV.dial :air1 @20:48:21.73 AS.reconfigure ASslow1 @20:48:21.78 AS.seek 0.587ml @20:48:28.10 ATV.dial :waste @20:48:28.65 AS.reconfigure ASconfig @20:48:28.71 AS.seek :empty @20:49:02.82 AFV.select :carrierSolution @20:49:02.93 delay 2 seconds @20:49:04.96 AS.seek 0.5ml @20:49:34.06 delay 1 second @20:49:35.08 AFV.select :holdingCoil @20:49:35.19 ATV.dial :air1 @20:49:35.80 AS.reconfigure ASslow1 @20:49:35.85 AS.seek 0.52ml @20:49:40.17 delay 5 seconds @20:49:45.19 ATV.dial :extSrc @20:49:46.93 AS.reconfigure ASslow2 @20:49:46.98 AS.seek 0.538ml @20:50:05.32 delay 10 seconds @20:50:15.35 ATV.dial :air1 @20:50:17.10 AS.reconfigure ASslow1 @20:50:17.15 AS.seek 0.568ml @20:50:23.46 ATV.dial :waste @20:50:24.03 AS.reconfigure ASconfig @20:50:24.09 AS.seek :empty @20:50:57.12 deconEluateRecoveryLine extSrc @20:50:57.13 ATV.dial :waste @20:50:57.16 AS.reconfigure ASnorm @20:50:57.23 AS.seek 0ml @20:50:57.59 ATV.dial :air3 @20:50:58.29 AS.reconfigure ASslow1 @20:50:58.35 AS.seek 0.01ml @20:51:00.67 delay 2 seconds @20:51:02.69 ATV.dial :bleach @20:51:03.16 AS.seek 0.31ml @20:52:03.76 delay 3 seconds @20:52:06.78 ATV.dial :air3 @20:52:07.34 delay 3 seconds @20:52:10.41 AS.seek 0.335ml @20:52:15.71 delay 3 seconds @20:52:18.73 ATV.dial :bleach @20:52:19.35 AS.seek 0.32ml @20:52:22.63 delay 3 seconds @20:52:25.65 ATV.dial :extSrc @20:52:26.30 AS.seek 0ml @20:53:30.90 AFV.select :carrierSolution @20:53:31.01 delay 2 seconds @20:53:33.04 AS.reconfigure ASconfig @20:53:33.09 AS.seek 0.5ml @20:54:02.13 delay 1 second @20:54:03.15 AFV.select :holdingCoil @20:54:03.25 ATV.dial :extSrc @20:54:03.27 AS.reconfigure ASslow1 @20:54:03.34 AS.seek 0ml @20:55:44.17 AFV.select :carrierSolution @20:55:44.28 delay 2 seconds @20:55:46.31 AS.reconfigure ASconfig @20:55:46.36 AS.seek 0.5ml @20:56:15.38 delay 1 second @20:56:16.40 AFV.select :holdingCoil @20:56:16.50 ATV.dial :extSrc @20:56:16.52 AS.reconfigure ASslow1 @20:56:16.59 AS.seek 0ml @20:57:57.43 AFV.select :carrierSolution @20:57:57.54 delay 2 seconds @20:57:59.57 AS.reconfigure ASconfig @20:57:59.62 AS.seek 0.5ml @20:58:28.65 delay 1 second @20:58:29.67 AFV.select :holdingCoil @20:58:29.77 ATV.dial :extSrc @20:58:29.79 AS.reconfigure ASslow1 @20:58:29.86 AS.seek 0ml @21:00:10.70 AFV.select :carrierSolution @21:00:10.81 delay 2 seconds @21:00:12.84 AS.reconfigure ASconfig @21:00:12.89 AS.seek 0.5ml @21:00:41.95 delay 1 second @21:00:42.97 AFV.select :holdingCoil @21:00:43.07 ATV.dial :extSrc @21:00:43.09 AS.reconfigure ASslow1 @21:00:43.16 AS.seek 0ml @21:02:24.01 ATV.dial :air2 @21:02:25.37 AS.reconfigure ASslow0 @21:02:25.42 AS.seek 0.5ml @21:03:16.01 ATV.dial :extSrc @21:03:17.52 AS.seek 0.02ml @21:04:06.12 delay 1 second @21:04:07.14 ATV.dial :waste @21:04:08.46 AS.reconfigure ASconfig @21:04:08.52 AS.seek :empty @21:04:10.03 deconEluateRecoveryLine extSrc @21:04:10.04 ATV.dial :waste @21:04:10.08 AS.reconfigure ASnorm @21:04:10.14 AS.seek 0ml @21:04:10.51 ATV.dial :air3 @21:04:11.37 AS.reconfigure ASslow1 @21:04:11.43 AS.seek 0.01ml @21:04:13.73 delay 2 seconds @21:04:15.75 ATV.dial :bleach @21:04:16.61 AS.seek 0.31ml @21:05:17.21 delay 3 seconds @21:05:20.23 ATV.dial :air3 @21:05:20.79 delay 3 seconds @21:05:23.86 AS.seek 0.335ml @21:05:29.19 delay 3 seconds @21:05:32.21 ATV.dial :bleach @21:05:32.84 AS.seek 0.32ml @21:05:36.15 delay 3 seconds @21:05:39.17 ATV.dial :extSrc @21:05:39.82 AS.seek 0ml @21:06:44.42 AFV.select :carrierSolution @21:06:44.53 delay 2 seconds @21:06:46.56 AS.reconfigure ASconfig @21:06:46.61 AS.seek 0.5ml @21:07:15.67 delay 1 second @21:07:16.69 AFV.select :holdingCoil @21:07:16.79 ATV.dial :extSrc @21:07:16.81 AS.reconfigure ASslow1 @21:07:16.88 AS.seek 0ml @21:08:57.68 AFV.select :carrierSolution @21:08:57.79 delay 2 seconds @21:08:59.82 AS.reconfigure ASconfig @21:08:59.87 AS.seek 0.5ml @21:09:28.92 delay 1 second @21:09:29.94 AFV.select :holdingCoil @21:09:30.04 ATV.dial :extSrc @21:09:30.06 AS.reconfigure ASslow1 @21:09:30.13 AS.seek 0ml @21:11:10.95 AFV.select :carrierSolution @21:11:11.06 delay 2 seconds @21:11:13.09 AS.reconfigure ASconfig @21:11:13.14 AS.seek 0.5ml @21:11:42.18 delay 1 second @21:11:43.20 AFV.select :holdingCoil @21:11:43.30 ATV.dial :extSrc @21:11:43.32 AS.reconfigure ASslow1 @21:11:43.39 AS.seek 0ml @21:13:24.23 AFV.select :carrierSolution @21:13:24.34 delay 2 seconds @21:13:26.37 AS.reconfigure ASconfig @21:13:26.42 AS.seek 0.5ml @21:13:55.46 delay 1 second @21:13:56.48 AFV.select :holdingCoil @21:13:56.58 ATV.dial :extSrc @21:13:56.60 AS.reconfigure ASslow1 @21:13:56.67 AS.seek 0ml @21:15:37.49 ATV.dial :air2 @21:15:38.87 AS.reconfigure ASslow0 @21:15:38.93 AS.seek 0.5ml @21:16:29.57 ATV.dial :extSrc @21:16:31.10 AS.seek 0.02ml @21:17:19.70 delay 1 second @21:17:20.72 ATV.dial :waste @21:17:22.04 AS.reconfigure ASconfig @21:17:22.10 AS.seek :empty @21:17:23.60 deconEluateRecoveryLine extSrc @21:17:23.61 ATV.dial :waste @21:17:23.65 AS.reconfigure ASnorm @21:17:23.71 AS.seek 0ml @21:17:24.07 ATV.dial :air3 @21:17:24.94 AS.reconfigure ASslow1 @21:17:25.00 AS.seek 0.01ml @21:17:27.29 delay 2 seconds @21:17:29.31 ATV.dial :bleach @21:17:29.77 AS.seek 0.31ml @21:18:30.37 delay 3 seconds @21:18:33.39 ATV.dial :air3 @21:18:33.95 delay 3 seconds @21:18:37.02 AS.seek 0.335ml @21:18:42.34 delay 3 seconds @21:18:45.36 ATV.dial :bleach @21:18:45.99 AS.seek 0.32ml @21:18:49.31 delay 3 seconds @21:18:52.33 ATV.dial :extSrc @21:18:52.96 AS.seek 0ml @21:19:57.57 AFV.select :carrierSolution @21:19:57.68 delay 2 seconds @21:19:59.71 AS.reconfigure ASconfig @21:19:59.77 AS.seek 0.5ml @21:20:28.81 delay 1 second @21:20:29.83 AFV.select :holdingCoil @21:20:29.93 ATV.dial :extSrc @21:20:29.95 AS.reconfigure ASslow1 @21:20:30.02 AS.seek 0ml @21:22:10.85 AFV.select :carrierSolution @21:22:10.96 delay 2 seconds @21:22:12.99 AS.reconfigure ASconfig @21:22:13.04 AS.seek 0.5ml @21:22:42.07 delay 1 second @21:22:43.09 AFV.select :holdingCoil @21:22:43.19 ATV.dial :extSrc @21:22:43.21 AS.reconfigure ASslow1 @21:22:43.28 AS.seek 0ml @21:24:24.17 AFV.select :carrierSolution @21:24:24.28 delay 2 seconds @21:24:26.31 AS.reconfigure ASconfig @21:24:26.36 AS.seek 0.5ml @21:24:55.40 delay 1 second @21:24:56.42 AFV.select :holdingCoil @21:24:56.52 ATV.dial :extSrc @21:24:56.54 AS.reconfigure ASslow1 @21:24:56.61 AS.seek 0ml @21:26:37.48 AFV.select :carrierSolution @21:26:37.59 delay 2 seconds @21:26:39.62 AS.reconfigure ASconfig @21:26:39.67 AS.seek 0.5ml @21:27:08.70 delay 1 second @21:27:09.72 AFV.select :holdingCoil @21:27:09.82 ATV.dial :extSrc @21:27:09.84 AS.reconfigure ASslow1 @21:27:09.90 AS.seek 0ml @21:28:50.74 ATV.dial :air2 @21:28:52.48 AS.reconfigure ASslow0 @21:28:52.54 AS.seek 0.5ml @21:29:43.17 ATV.dial :extSrc @21:29:44.69 AS.seek 0.02ml @21:30:33.31 delay 1 second @21:30:34.33 ATV.dial :waste @21:30:35.63 AS.reconfigure ASconfig @21:30:35.69 AS.seek :empty @21:30:37.25 Session Ended @18:13:18.37CEST14-May-19
ESP2 v3.30 I2C Gateway v3.06 for core bus @18:13:20.10 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @18:13:20.35 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @18:13:25.50 Polling Can every 10:00 @18:13:27.23 SPE.coast @18:13:27.28 SPE.configure SPEconfig @18:13:27.41 AS.configure ASconfig @18:13:27.59 SS.reconfigure SSconfig @18:13:27.65 SS.hold @18:13:27.75 SS.configure SSconfig @18:13:27.93 CC.reconfigure CCconfig @18:13:27.99 CC.hold @18:13:28.11 CC.configure CCconfig @18:13:28.19 CS.reconfigure CSconfig @18:13:28.23 CS.hold @18:13:28.30 CS.configure CSconfig @18:13:28.38 PC.reconfigure PCconfig @18:13:28.42 PC.hold @18:13:28.82 PC.configure PCconfig @18:13:28.90 PS.configure PSconfig @18:13:29.00 Forearm.reconfigure LooseForearm @18:13:29.04 Forearm.hold @18:13:29.10 Forearm.configure LooseForearm @18:13:29.21 Elbow.reconfigure LooseElbow @18:13:29.27 Elbow.hold @18:13:29.37 Elbow.configure LooseElbow @18:13:29.57 SC.reconfigure SCloose @18:13:29.65 SC.hold @18:13:29.75 SC.configure SCloose @18:13:29.91 SE.reconfigure SEconfig @18:13:29.98 SE.hold @18:13:30.04 SE.configure SEconfig @18:13:30.08 CH.coast @18:13:30.12 CH.configure CHconfig @18:13:30.15 PH.coast @18:13:30.20 PH.configure PHconfig @18:13:30.23 PCR.off @18:13:30.25 PCR.pidtemp= 50,0.1,0,300,25,25 @18:13:30.28 PCR.caltemp= 2.8814,0.0372,0 @18:13:30.31 All dwarf controllers running firmware version 3.75 @18:13:30.42 Cleanly exited at 17:51:09CEST14-May-19 @18:13:30.45 Loading /home/esp/esp2/mission/18tun.rb mission @18:13:30.51 Preparing to sleep until +0:00:15 @18:13:30.54 GoodNews.email "Starting 18tun w/pucks in tube 2\n{}\nCan@18:13:25, 20.9C, 23% humidity, 14.8psia, 11.996V, 0.664A, 0.664A avg, 7.96W",Subject: "18tun begun" @18:13:30.65 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @18:13:30.68 Retry #1 of 10 after 10:00 @18:13:30.72 SS.coast @18:13:30.78
Asleep til +0:00:15 @18:13:30.83 Continuing at 18:13 @18:13:30.88 CC.coast @18:13:31.04 CS.coast @18:13:31.10 PC.coast @18:13:31.16 Forearm.coast @18:13:31.22 Elbow.coast @18:13:31.28 SC.coast @18:13:31.34 SE.coast @18:13:31.56 Session Begun @18:13:31.79 Gate.power :core,:OFF @18:13:31.82 Gate.power :raw,:OFF @18:13:31.84 Shutdown @18:13:42.92 -> showlog 100 @18:13:45.48
Powering core bus @18:13:50.47 Gate.power :raw,:ON @18:13:51.04 SPE.coast @18:13:51.11 SPE.configure SPEconfig @18:13:51.20 AS.configure ASconfig @18:13:51.31 SS.configure SSconfig @18:13:51.41 CC.configure CCconfig @18:13:51.51 CS.configure CSconfig @18:13:51.61 PC.configure PCconfig @18:13:51.71 PS.configure PSconfig @18:13:51.83 Forearm.configure LooseForearm @18:13:51.96 Elbow.configure LooseElbow @18:13:52.08 SC.configure SCloose @18:13:52.18 SE.configure SEconfig @18:13:52.23 CH.coast @18:13:52.29 CH.configure CHconfig @18:13:52.33 PH.coast @18:13:52.40 PH.configure PHconfig @18:13:52.45 PCR.off @18:13:52.49 PCR.pidtemp= 50,0.1,0,300,25,25 @18:13:52.53 PCR.caltemp= 2.8814,0.0372,0 @18:13:52.56 All dwarf controllers running firmware version 3.75 @18:13:52.68 Valve.reset @18:13:52.86 PCR.off @18:13:52.89 PCR.pidtemp= 50,0.1,0,300,25,25 @18:13:52.92 PCR.caltemp= 2.8814,0.0372,0 @18:13:53.08 Intake.select :close @18:13:53.10 Exhaust.select :close @18:13:53.12 CTV.dial :air @18:13:53.28 PSV.dial :air @18:13:53.38 CSV.dial :waste1 @18:13:53.41
Forearm.reconfigure LooseForearm @18:13:53.55 PS.reconfigure PSconfig @18:13:53.56 SSV.dial :spit,avoiding: :exhaust @18:13:53.59
Forearm.home.seek :retract @18:13:53.71 PS.home.seek 0ml @18:13:53.86 CS.reconfigure CSfast @18:13:53.89 SS.reconfigure SSconfig @18:13:53.99 CS.home.seek 0ml @18:13:54.06 SS.home.seek 0ml @18:13:54.39 Hand.close @18:13:54.45
Elbow.jog 1500 @18:13:54.95 Elbow.reconfigure LooseElbow @18:13:55.01 Elbow.home.seek :clear @18:13:59.68 SE.reconfigure SEconfig @18:13:59.74 SE.home.seek :down @18:14:00.47 SC.reconfigure SCsloppy @18:14:00.53 SC.home.seek :initial @18:14:02.97 PS.coast @18:14:11.03
Forearm.seek :retract @18:14:11.12 Elbow.seek :hammer @18:14:12.28 Forearm.reconfigure TightForearm @18:14:12.34 Forearm.seek 5 + 350 counts @18:14:13.43 Forearm.seek :retract @18:14:14.72 PC.reconfigure PCconfig @18:14:14.74
CC.reconfigure CCconfig @18:14:14.83 PC.home.seek :open @18:14:14.86
CC.home.seek :open @18:14:18.17 Phase [coreNEG] @18:14:18.71 Readying the MFB @18:14:18.72 Homing MFB Syringe @18:14:18.74 AFV.select :holdingCoil @18:14:18.89 ATV.dial :air2 @18:14:20.45 AS.reconfigure ASconfig @18:14:20.51 AS.home.seek :home @18:14:26.00 AFV.select :carrierSolution @18:14:26.11 delay 2 seconds @18:14:28.14 AS.seek 1ml @18:15:21.04 delay 1 second @18:15:22.06 AFV.select :holdingCoil @18:15:22.16 delay 1 second @18:15:23.18 ATV.dial :waste @18:15:24.28 AS.reconfigure ASnorm @18:15:24.34 AS.home.seek :empty @18:16:16.90 ATV.dial :waste @18:16:16.91 ARV.dial between carrier and air1 @18:16:17.01 ASV.dial :waste1 @18:16:17.11 Full MFB Decontamination @18:16:17.12 Decon extraction column and decon AIV @18:16:17.16 SPE.hold 2:46:40,55C @18:16:17.21 ASV.dial :SPEdelivery @18:16:17.64 delay 20 seconds @18:16:37.67 AFV.select :carrierSolution @18:16:37.77 delay 2 seconds @18:16:39.80 AS.reconfigure ASconfig @18:16:39.85 AS.seek 0.5ml @18:17:08.94 delay 1 second @18:17:09.96 AFV.select :holdingCoil @18:17:10.06 ATV.dial :air1 @18:17:10.73 AS.reconfigure ASslow1 @18:17:10.79 AS.seek 0.52ml @18:17:15.08 ATV.dial :ASV @18:17:16.81 ASV.dial :SPErecovery @18:17:17.51 AS.seek 0.62ml @18:17:37.90 ATV.dial :waste @18:17:40.06 AS.reconfigure ASnorm @18:17:40.12 AS.seek :empty @18:18:11.78 AFV.select :holdingCoil @18:18:11.79 ATV.dial :waste @18:18:11.82 AS.reconfigure ASconfig @18:18:11.88 AS.seek 0ml @18:18:12.19 AFV.select :carrierSolution @18:18:12.30 delay 2 seconds @18:18:14.33 AS.seek 0.5ml @18:18:43.36 delay 1 second @18:18:44.38 AFV.select :holdingCoil @18:18:44.48 ATV.dial :air3 @18:18:45.25 AS.reconfigure ASslow1 @18:18:45.31 AS.seek 0.52ml @18:18:49.64 ATV.dial :air3 @18:18:49.73 AS.seek 0.53ml @18:18:52.01 delay 2 seconds @18:18:54.03 ATV.dial :bleach @18:18:54.54 AS.seek 0.71ml @18:19:31.00 delay 3 seconds @18:19:34.02 ATV.dial :air3 @18:19:34.62 delay 3 seconds @18:19:37.69 AS.seek 0.735ml @18:19:43.01 delay 3 seconds @18:19:46.03 ATV.dial :bleach @18:19:46.70 AS.seek 0.72ml @18:19:50.01 delay 3 seconds @18:19:53.03 ATV.dial ASV @18:19:54.17 ASV.dial :SPErecovery @18:19:54.26 AS.seek 0.365ml @18:21:05.94 ATV.dial :waste @18:21:07.78 AS.reconfigure ASnorm @18:21:07.84 AS.seek :empty @18:21:26.64 ATV.dial :air3 @18:21:27.35 AS.reconfigure ASslow1 @18:21:27.41 AS.seek 0.009ml @18:21:29.84 delay 2 seconds @18:21:31.86 ATV.dial :bleach @18:21:32.35 AS.seek 0.579ml @18:23:27.20 delay 3 seconds @18:23:30.22 ATV.dial :air3 @18:23:30.71 GoodNews.email "Starting 18tun w/pucks in tube 2\n{}\nCan@18:13:25, 20.9C, 23% humidity, 14.8psia, 11.996V, 0.664A, 0.664A avg, 7.96W",Subject: "18tun begun" @18:23:30.75 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @18:23:30.77 Retry #2 of 10 after 10:00 @18:23:30.82
delay 3 seconds @18:23:33.90 AS.seek 0.604ml @18:23:39.23 delay 3 seconds @18:23:42.25 ATV.dial :bleach @18:23:42.89 AS.seek 0.589ml @18:23:46.22 delay 3 seconds @18:23:49.24 ATV.dial :ASV @18:23:50.34 ASV.dial :wasteR @18:23:50.84 AS.seek 0ml @18:25:49.59 delay 1:00 @18:26:49.62 ATV.dial ASV @18:26:49.63 AFV.select :carrierSolution @18:26:49.75 delay 2 seconds @18:26:51.78 AS.reconfigure ASconfig @18:26:51.83 AS.seek 1ml @18:27:49.59 delay 1 second @18:27:50.61 AFV.select :holdingCoil @18:27:50.71 delay 1 second @18:27:51.73 ASV.dial :wasteR @18:27:51.75 AS.reconfigure ASslow1 @18:27:51.82 AS.seek 0ml @18:31:13.23 delay 10 seconds @18:31:23.25 ATV.dial :air3 @18:31:24.82 AS.seek 0.01ml @18:31:27.11 delay 2 seconds @18:31:29.13 ATV.dial :bleach @18:31:29.79 AS.seek 0.58ml @18:33:24.72 delay 3 seconds @18:33:27.74 ATV.dial :air3 @18:33:28.33 delay 3 seconds @18:33:30.81 GoodNews.email "Starting 18tun w/pucks in tube 2\n{}\nCan@18:13:25, 20.9C, 23% humidity, 14.8psia, 11.996V, 0.664A, 0.664A avg, 7.96W",Subject: "18tun begun" @18:33:30.86 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @18:33:30.87 Retry #3 of 10 after 10:00 @18:33:31.40
AS.seek 0.605ml @18:33:36.72 delay 3 seconds @18:33:39.74 ATV.dial :bleach @18:33:40.39 AS.seek 0.59ml @18:33:43.72 delay 3 seconds @18:33:46.74 ATV.dial ASV @18:33:48.23 ASV.dial :SPEdelivery @18:33:48.63 AS.seek 0ml @18:35:47.59 delay 1:00 @18:36:47.62 AFV.select :carrierSolution @18:36:47.72 delay 2 seconds @18:36:49.75 AS.reconfigure ASconfig @18:36:49.81 AS.seek 1ml @18:37:47.58 delay 1 second @18:37:48.60 AFV.select :holdingCoil @18:37:48.70 delay 1 second @18:37:49.72 ATV.dial ASV @18:37:49.73 ASV.dial :SPEdelivery @18:37:49.76 AS.reconfigure ASslow1 @18:37:49.82 AS.seek 0ml @18:41:11.25 delay 10 seconds @18:41:21.28 ATV.dial :air2 @18:41:22.16 AS.reconfigure ASslow0 @18:41:22.22 AS.seek 0.8ml @18:42:43.03 delay 3 seconds @18:42:46.05 ATV.dial :ASV @18:42:47.08 ASV.dial :SPEdelivery @18:42:47.10 AS.reconfigure ASslow1 @18:42:47.17 AS.seek 0.03ml @18:43:30.90 GoodNews.email "Starting 18tun w/pucks in tube 2\n{}\nCan@18:13:25, 20.9C, 23% humidity, 14.8psia, 11.996V, 0.664A, 0.664A avg, 7.96W",Subject: "18tun begun" @18:43:30.94 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @18:43:30.96 Retry #4 of 10 after 10:00 @18:45:22.34
delay 20 seconds @18:45:42.37 ATV.dial :waste,via: :air3 @18:45:44.17 AS.reconfigure ASconfig @18:45:44.23 AS.seek :empty @18:45:46.33 SPE.coast @18:45:46.41 Removing Bleach from Mixing Coil @18:45:46.42 AFV.select :carrierSolution @18:45:46.53 delay 2 seconds @18:45:48.56 AS.seek 0.5ml @18:46:17.69 delay 1 second @18:46:18.71 AFV.select :holdingCoil @18:46:18.81 ATV.dial :ASV @18:46:20.70 ASV.dial :SPErecovery @18:46:21.37 AS.reconfigure ASslow1 @18:46:21.43 AS.seek 0.73ml @18:47:07.95 ASV.dial :wasteD @18:47:08.53 AS.reconfigure ASnorm @18:47:08.59 AS.seek 0ml @18:47:45.72 AFV.select :carrierSolution @18:47:45.83 delay 2 seconds @18:47:47.86 AS.reconfigure ASconfig @18:47:47.91 AS.seek 0.5ml @18:48:16.97 delay 1 second @18:48:17.99 AFV.select :holdingCoil @18:48:18.09 ATV.dial ASV @18:48:18.10 ASV.dial :SPErecovery @18:48:18.76 AS.reconfigure ASslow0 @18:48:18.82 AS.seek 0.3ml @18:48:39.25 ATV.dial :air2 @18:48:40.17 AS.reconfigure ASslow1 @18:48:40.23 AS.seek 0.32ml @18:48:44.61 ATV.dial ASV @18:48:45.68 AS.reconfigure ASslow0 @18:48:45.74 AS.seek 0.57ml @18:49:11.19 ASV.dial :wasteD @18:49:11.78 AS.reconfigure ASnorm @18:49:11.84 AS.seek 0ml @18:49:40.92 AFV.select :carrierSolution @18:49:41.03 delay 2 seconds @18:49:43.06 AS.reconfigure ASconfig @18:49:43.11 AS.seek 0.5ml @18:50:12.14 delay 1 second @18:50:13.16 AFV.select :holdingCoil @18:50:13.26 ATV.dial ASV @18:50:13.27 ASV.dial :SPErecovery @18:50:13.93 AS.reconfigure ASslow0 @18:50:13.99 AS.seek 0.3ml @18:50:34.39 ATV.dial :air2 @18:50:35.45 AS.reconfigure ASslow1 @18:50:35.51 AS.seek 0.32ml @18:50:39.86 ATV.dial ASV @18:50:40.92 AS.reconfigure ASslow0 @18:50:40.98 AS.seek 0.57ml @18:51:06.45 ASV.dial :wasteD @18:51:07.04 AS.reconfigure ASnorm @18:51:07.10 AS.seek 0ml @18:51:36.19 ATV.dial :air2 @18:51:37.20 AS.reconfigure ASslow0 @18:51:37.25 AS.seek 0.3ml @18:52:07.79 ATV.dial :ASV @18:52:08.78 ASV.dial :wasteD @18:52:08.80 AS.reconfigure ASconfig @18:52:08.86 AS.seek 0.05ml @18:52:23.56 ATV.dial :waste @18:52:25.49 AS.seek 0ml @18:52:28.66 ASV.dial :SPEdelivery @18:52:29.67 deconLysateINT @18:52:29.68 ATV.dial :air3 @18:52:30.40 AS.reconfigure ASslow1 @18:52:30.46 AS.seek 0.01ml @18:52:32.76 delay 2 seconds @18:52:34.78 ATV.dial :bleach @18:52:35.25 AS.seek 0.96ml @18:53:30.99 GoodNews.email "Starting 18tun w/pucks in tube 2\n{}\nCan@18:13:25, 20.9C, 23% humidity, 14.8psia, 11.996V, 0.664A, 0.664A avg, 7.96W",Subject: "18tun begun" @18:53:31.03 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @18:53:31.05 Retry #5 of 10 after 10:00 @18:55:46.56
delay 3 seconds @18:55:49.58 ATV.dial :air3 @18:55:50.15 delay 3 seconds @18:55:53.22 AS.seek 0.985ml @18:55:58.54 delay 3 seconds @18:56:01.56 ATV.dial :bleach @18:56:02.20 AS.seek 0.97ml @18:56:05.62 delay 3 seconds @18:56:08.64 ATV.dial AIV @18:56:10.03 AIV.dial :coilToMFB @18:56:10.11 AWV.select :waste @18:56:10.22 AFV.select :carrierSolution @18:56:10.33 delay 2 seconds @18:56:12.36 AS.reconfigure ASconfig @18:56:12.41 AS.seek 1ml @18:56:14.45 delay 1 second @18:56:15.47 AFV.select :holdingCoil @18:56:15.58 delay 1 second @18:56:16.61 AS.reconfigure ASslow0 @18:56:16.66 AS.seek :empty @18:57:57.62 AFV.select :carrierSolution @18:57:57.73 delay 2 seconds @18:57:59.76 AS.reconfigure ASconfig @18:57:59.81 AS.seek 1ml @18:58:57.62 delay 1 second @18:58:58.64 AFV.select :holdingCoil @18:58:58.74 delay 1 second @18:58:59.77 AS.reconfigure ASslow0 @18:58:59.82 AS.seek :empty @19:00:40.86 AFV.select :carrierSolution @19:00:40.97 delay 2 seconds @19:00:43.00 AS.reconfigure ASconfig @19:00:43.05 AS.seek 1ml @19:01:41.28 delay 1 second @19:01:42.30 AFV.select :holdingCoil @19:01:42.40 delay 1 second @19:01:43.43 AS.reconfigure ASslow0 @19:01:43.48 AS.seek :empty @19:03:24.40 ATV.dial :air2 @19:03:25.11 AS.reconfigure ASslow1 @19:03:25.17 AS.seek 0.009ml @19:03:27.61 AS.reconfigure ASslow0 @19:03:27.67 AS.seek 1ml @19:03:31.08 GoodNews.email "Starting 18tun w/pucks in tube 2\n{}\nCan@18:13:25, 20.9C, 23% humidity, 14.8psia, 11.996V, 0.664A, 0.664A avg, 7.96W",Subject: "18tun begun" @19:03:31.12 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:03:31.14 Retry #6 of 10 after 10:00 @19:05:07.64
ATV.dial AIV @19:05:08.47 AS.seek 0.02ml @19:06:47.37 ATV.dial :waste @19:06:49.07 AS.seek 0ml @19:06:51.42 AWV.select :air @19:06:51.58 Cleaning PCR @19:06:51.59 AFV.select :carrierSolution @19:06:51.70 delay 2 seconds @19:06:53.73 AS.reconfigure ASconfig @19:06:53.78 AS.seek 0.5ml @19:07:22.81 delay 1 second @19:07:23.83 AFV.select :holdingCoil @19:07:23.93 ASV.dial :waste1 @19:07:24.30 ATV.dial :air3 @19:07:25.00 AS.reconfigure ASslow1 @19:07:25.06 AS.seek 0.51ml @19:07:27.37 delay 2 seconds @19:07:29.39 ATV.dial :bleach @19:07:29.87 AS.seek 0.81ml @19:08:30.45 delay 3 seconds @19:08:33.47 ATV.dial :air3 @19:08:34.05 delay 3 seconds @19:08:37.13 AS.seek 0.835ml @19:08:42.45 delay 3 seconds @19:08:45.47 ATV.dial :bleach @19:08:46.09 AS.seek 0.82ml @19:08:49.40 delay 3 seconds @19:08:52.42 ARV.dial :waste @19:08:52.92 delay 3 seconds @19:08:55.94 ATV.dial :ARV @19:08:56.86 AS.reconfigure ASconfig @19:08:56.92 AS.seek 0.47ml @19:09:17.34 AFV.select :carrierSolution @19:09:17.45 delay 2 seconds @19:09:19.48 AS.seek 1ml @19:09:50.23 delay 1 second @19:09:51.25 AFV.select :holdingCoil @19:09:51.35 delay 1 second @19:09:52.38 AS.reconfigure ASfast @19:09:52.43 AS.seek 0ml @19:10:19.80 AFV.select :carrierSolution @19:10:19.91 delay 2 seconds @19:10:21.94 AS.reconfigure ASconfig @19:10:21.99 AS.seek 1ml @19:11:19.80 delay 1 second @19:11:20.82 AFV.select :holdingCoil @19:11:20.92 delay 1 second @19:11:21.95 AS.reconfigure ASfast @19:11:22.00 AS.seek 0ml @19:11:49.33 AFV.select :carrierSolution @19:11:49.44 delay 2 seconds @19:11:51.47 AS.reconfigure ASconfig @19:11:51.52 AS.seek 1ml @19:12:49.33 delay 1 second @19:12:50.35 AFV.select :holdingCoil @19:12:50.45 delay 1 second @19:12:51.48 AS.reconfigure ASfast @19:12:51.53 AS.seek 0ml @19:13:18.89 ARV.dial between carrier and air1 @19:13:19.39 ATV.dial :air3 @19:13:20.72 AS.reconfigure ASslow1 @19:13:20.78 AS.seek 0.01ml @19:13:23.14 delay 2 seconds @19:13:25.16 ATV.dial :bleach @19:13:25.79 AS.seek 0.81ml @19:13:31.17 GoodNews.email "Starting 18tun w/pucks in tube 2\n{}\nCan@18:13:25, 20.9C, 23% humidity, 14.8psia, 11.996V, 0.664A, 0.664A avg, 7.96W",Subject: "18tun begun" @19:13:31.21 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:13:31.23 Retry #7 of 10 after 10:00 @19:16:06.92
delay 3 seconds @19:16:09.94 ATV.dial :air3 @19:16:10.50 delay 3 seconds @19:16:13.57 AS.seek 0.835ml @19:16:18.88 delay 3 seconds @19:16:21.90 ATV.dial :bleach @19:16:22.53 AS.seek 0.82ml @19:16:25.84 delay 3 seconds @19:16:28.86 ATV.dial PCR @19:16:29.28 AS.reconfigure ASconfig @19:16:29.34 AS.seek :empty @19:17:16.83 PCR.program :bleachProfile @19:17:16.88 PCR program should complete in < 2:45 @19:17:16.89 PCR.run 10:00 @19:20:18.72 PCR.off @19:20:18.75 AFV.select :carrierSolution @19:20:18.87 delay 2 seconds @19:20:20.90 AS.seek 1ml @19:21:18.76 delay 1 second @19:21:19.78 AFV.select :holdingCoil @19:21:19.88 delay 1 second @19:21:20.91 AS.reconfigure ASfast @19:21:20.96 AS.seek 0ml @19:21:48.33 delay 3 seconds @19:21:51.35 AFV.select :carrierSolution @19:21:51.45 delay 2 seconds @19:21:53.48 AS.reconfigure ASconfig @19:21:53.54 AS.seek 1ml @19:22:51.33 delay 1 second @19:22:52.35 AFV.select :holdingCoil @19:22:52.45 delay 1 second @19:22:53.48 AS.reconfigure ASfast @19:22:53.53 AS.seek 0ml @19:23:20.87 delay 3 seconds @19:23:23.89 AFV.select :carrierSolution @19:23:24.00 delay 2 seconds @19:23:26.03 AS.reconfigure ASconfig @19:23:26.10 AS.seek 1ml @19:23:31.26 GoodNews.email "Starting 18tun w/pucks in tube 2\n{}\nCan@18:13:25, 20.9C, 23% humidity, 14.8psia, 11.996V, 0.664A, 0.664A avg, 7.96W",Subject: "18tun begun" @19:23:31.31 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:23:31.33 Retry #8 of 10 after 10:00 @19:24:23.91
delay 1 second @19:24:24.93 AFV.select :holdingCoil @19:24:25.03 delay 1 second @19:24:26.06 AS.reconfigure ASfast @19:24:26.11 AS.seek 0ml @19:24:53.47 delay 3 seconds @19:24:56.49 AFV.select :carrierSolution @19:24:56.59 delay 2 seconds @19:24:58.62 AS.reconfigure ASconfig @19:24:58.67 AS.seek 1ml @19:25:56.84 delay 1 second @19:25:57.86 AFV.select :holdingCoil @19:25:57.96 delay 1 second @19:25:58.99 AS.reconfigure ASfast @19:25:59.04 AS.seek 0ml @19:26:26.37 delay 3 seconds @19:26:29.39 AFV.select :carrierSolution @19:26:29.49 delay 2 seconds @19:26:31.52 AS.reconfigure ASconfig @19:26:31.58 AS.seek 1ml @19:27:29.37 delay 1 second @19:27:30.39 AFV.select :holdingCoil @19:27:30.49 delay 1 second @19:27:31.52 AS.reconfigure ASnorm @19:27:31.57 AS.seek 0ml @19:28:22.34 AFV.select :carrierSolution @19:28:22.45 delay 2 seconds @19:28:24.48 AS.reconfigure ASconfig @19:28:24.53 AS.seek 1ml @19:29:22.31 delay 1 second @19:29:23.33 AFV.select :holdingCoil @19:29:23.43 delay 1 second @19:29:24.45 ATV.dial :waste @19:29:25.73 AS.reconfigure ASnorm @19:29:25.79 AS.seek 0.5ml @19:29:51.36 ATV.dial :waste2 @19:29:52.68 AS.seek 0ml @19:30:18.20CEST14-May-19 ==================> STARTED coreNEG (phase #1) <=== @19:30:18.30 Starting mfb @19:30:18.31 pcrBAC @19:30:18.38 Loading sh1 puck from tube 2 into Collection Clamp @19:30:18.49 Hand.open @19:30:19.41 SC.reconfigure SCloose @19:30:19.57 Elbow.reconfigure TightElbow @19:30:19.63 Elbow.seek 2 @19:30:20.08 Forearm.seek 2 + 700 counts @19:30:21.16 SE.reconfigure SEpick @19:30:21.25 SE.seek beamBreak @19:30:24.31 SE.configure SEconfig @19:30:24.48 Elbow.seek 2 @19:30:24.66 Forearm.seek 2 + 300 counts @19:30:24.93 Forearm.reconfigure LooseForearm @19:30:24.99 Hand.close @19:30:25.73 SE.seek :down @19:30:25.93
Forearm.seek :retract @19:30:26.93 Elbow.seek CC @19:30:28.45 SE.coast @19:30:28.63
Elbow.reconfigure LooseElbow @19:30:28.73 Forearm.reconfigure TightForearm @19:30:28.79 Forearm.seek CC @19:30:30.12 CC.seek :closed @19:30:34.39 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.33 @19:30:34.40 Hand.open @19:30:35.28 Forearm.reconfigure LooseForearm @19:30:35.34 Forearm.seek :retract @19:30:36.40 SC.coast @19:30:36.45 skipping prime and sample collection @19:30:36.46 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @19:30:36.48 Intake.select :close @19:30:36.58 CSV.dial :waste @19:30:37.06 CS.seek 0ml @19:30:37.15 CSV.dial :air @19:30:37.24
Calibrating pressure and temperature sensors @19:30:37.26 CTV.dial :air @19:30:37.29 SSV.dial :spit,avoiding: :exhaust @19:30:37.32 SS.reconfigure SSfast @19:30:37.39 SS.seek 5ml @19:30:38.16 CS.seek 10ml @19:30:40.58 SSV.dial CBV @19:30:40.61
CBV.dial SSV @19:30:42.14 delay 3.8 seconds @19:30:46.07 SSV.dial :spit @19:30:47.59 SS.seek 0ml @19:30:50.40 SSV.dial CBV @19:30:51.90 SSV.dial CBV,avoiding: :exhaust @19:30:51.93
CBV.dial SSV @19:30:51.96 CTV.dial :air @19:30:51.99 delay 15 seconds @19:31:07.01 SSV.dial :spit,avoiding: :exhaust @19:31:08.53 CTV.dial CSV,avoiding: :intake @19:31:08.57
SS.reconfigure SSexhale @19:31:08.68 SS.seek 0ml @19:31:08.74 SSV.dial CBV,avoiding: :exhaust @19:31:10.35 CBV.dial between SSV and waste2 @19:31:10.79 CTV.dial CSV,avoiding: :air @19:31:10.82
CSV.dial CTV @19:31:11.38 CS.reconfigure CSpressurize @19:31:11.44 CS.seek 0ml @19:31:37.62 CSV.dial :air @19:31:38.15 CS.reconfigure CSfast @19:31:38.22 CS.seek 10ml @19:32:04.96 CSV.dial between air and CTV @19:32:05.46 CS.seek 3.434ml @19:32:23.31 CTV.dial CSV,avoiding: :air @19:32:23.34
CSV.dial CTV @19:32:23.73 CS.reconfigure CSpressurize @19:32:23.79 CS.seek 0ml @19:32:33.37 CSV.dial :air @19:32:33.91 CS.reconfigure CSfast @19:32:33.98 CS.seek 10ml @19:33:00.59 CSV.dial between air and CTV @19:33:01.09 CS.seek 2.176ml @19:33:22.09 CTV.dial CSV,avoiding: :air @19:33:22.12
CSV.dial CTV @19:33:22.51 CS.reconfigure CSpressurize @19:33:22.57 CS.seek 0ml @19:33:24.38 BubblePoint: Intake @69.7psia, Outlet @14.3psia CBV.dial SSV @19:33:24.78 SS.reconfigure SSconfig @19:33:24.84 SS.seek 10ml @19:33:30.95 delay 15 seconds @19:33:31.36 GoodNews.email "Starting 18tun w/pucks in tube 2\n{}\nCan@18:13:25, 20.9C, 23% humidity, 14.8psia, 11.996V, 0.664A, 0.664A avg, 7.96W",Subject: "18tun begun" @19:33:31.40 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:33:31.42 Retry #9 of 10 after 10:00 @19:33:46.07
Evacuated: Intake @29.0psia, Outlet @29.0psia @19:33:46.08 SSV.dial :spit,avoiding: :exhaust @19:33:47.62 SS.reconfigure SSexhale @19:33:47.68 SS.seek 0ml @19:33:52.53 SS.reconfigure SSfast @19:33:52.59 SS.seek 5ml @19:33:56.04 SSV.dial CBV,avoiding: :exhaust @19:33:56.07
CBV.dial SSV @19:33:57.59 CTV.dial :air,avoiding: :intake @19:33:59.17 CSV.dial CTV @19:33:59.22
SS.seek 10ml @19:34:00.20 CSV.dial :air @19:34:00.72 CS.reconfigure CSfast @19:34:00.78 CS.seek 0ml @19:34:02.17
SSV.dial :spit,avoiding: :exhaust @19:34:03.68 SS.reconfigure SSexhale @19:34:03.74 SS.seek 0ml @19:34:06.34 CS.coast @19:34:08.77
SS.reconfigure SSfast @19:34:08.84 SS.seek 5ml @19:34:11.88 SSV.dial CBV,avoiding: :exhaust @19:34:11.91
CBV.dial SSV @19:34:14.43 SSV.dial :spit,avoiding: :exhaust @19:34:15.95 SS.reconfigure SSconfig @19:34:16.02 SS.seek 0ml @19:34:19.09 SS.coast @19:34:19.14 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @19:34:19.15 Intake.select :close @19:34:19.25 CSV.dial :waste @19:34:19.37 SSV.dial CBV,avoiding: :exhaust @19:34:19.39
CBV.dial SSV @19:34:20.17 CS.seek 0ml @19:34:20.25 CSV.dial :air @19:34:20.93
CTV.dial :air @19:34:20.94 delay 15 seconds @19:34:21.12 CS.seek 10ml @19:34:35.97
SSV.dial :spit,avoiding: :exhaust @19:34:37.50 CTV.dial CSV,avoiding: :intake @19:34:37.54
SS.reconfigure SSexhale @19:34:37.65 SS.seek 0ml @19:34:37.73 SSV.dial CBV,avoiding: :exhaust @19:34:47.84 CBV.dial between SSV and waste2 @19:34:48.28 CTV.dial CSV,avoiding: :air @19:34:48.31
CSV.dial CTV @19:34:48.85 CS.reconfigure CSpressurize @19:34:48.91 CS.seek 0ml @19:35:15.03 CSV.dial :air @19:35:15.56 CS.reconfigure CSfast @19:35:15.63 CS.seek 10ml @19:35:42.25 CSV.dial between air and CTV @19:35:42.62 CS.seek 3.417ml @19:36:00.34 CTV.dial CSV,avoiding: :air @19:36:00.37
CSV.dial CTV @19:36:00.74 CS.reconfigure CSpressurize @19:36:00.80 CS.seek 0ml @19:36:10.25 CSV.dial :air @19:36:10.78 CS.reconfigure CSfast @19:36:10.85 CS.seek 10ml @19:36:37.15 CSV.dial between air and CTV @19:36:37.53 CS.seek 2.165ml @19:36:58.56 CTV.dial CSV,avoiding: :air @19:36:58.60
CSV.dial CTV @19:36:58.95 CS.reconfigure CSpressurize @19:36:59.43 CS.seek 0ml @19:37:01.26 BubblePoint: Intake @70.2psia, Outlet @15.1psia @19:37:01.27 CBV.dial SSV @19:37:01.67 SS.reconfigure SSconfig @19:37:01.73 SS.seek 10ml @19:37:07.84 delay 15 seconds @19:37:22.96 Evacuated: Intake @29.1psia, Outlet @29.0psia @19:37:22.97 SSV.dial :spit,avoiding: :exhaust @19:37:24.49 SS.reconfigure SSexhale @19:37:24.55 SS.seek 0ml @19:37:29.44 SS.reconfigure SSfast @19:37:29.50 SS.seek 5ml @19:37:32.37 SSV.dial CBV,avoiding: :exhaust @19:37:32.40
CBV.dial SSV @19:37:33.93 CTV.dial :air,avoiding: :intake @19:37:35.50 CSV.dial CTV @19:37:35.55
SS.seek 10ml @19:37:36.53 CSV.dial :air @19:37:37.05 CS.reconfigure CSfast @19:37:37.11 CS.seek 0ml @19:37:38.37
SSV.dial :spit,avoiding: :exhaust @19:37:39.88 SS.reconfigure SSexhale @19:37:39.94 SS.seek 0ml @19:37:42.38 CS.coast @19:37:44.95
SS.reconfigure SSfast @19:37:45.02 SS.seek 5ml @19:37:48.04 SSV.dial CBV,avoiding: :exhaust @19:37:48.07
CBV.dial SSV @19:37:50.60 SSV.dial :spit,avoiding: :exhaust @19:37:52.12 SS.reconfigure SSconfig @19:37:52.19 SS.seek 0ml @19:37:55.23 SS.coast @19:37:55.28 Lysing: Adding 1.5ml lysis @19:37:55.29 CBV.dial between CSV and waste1,avoiding: :DBV @19:37:57.03 CTV.dial CSV @19:37:58.56 CSV.dial CSR @19:37:59.48 Pulling 1ml of CSR.lysis CSR.select :lysis @19:37:59.61 CS.reconfigure CSconfig @19:37:59.67 CS.seek 1ml @19:38:03.19 delay 5 seconds @19:38:08.21 CSR.select :air @19:38:08.39 CS.seek 6ml @19:38:22.22 delay 2 seconds @19:38:24.24 CSV.dial :waste1 @19:38:25.75 CS.reconfigure CSfast @19:38:25.81 CS.seek :empty @19:38:41.97 CSV.dial CSR @19:38:43.53 Pulling 1.5ml of CSR.lysis CSR.select :lysis @19:38:43.67 CS.reconfigure CSconfig @19:38:43.73 CS.seek 1.5ml @19:38:48.57 delay 5 seconds @19:38:53.59 CSR.select :air @19:38:53.77 CS.seek 4.5ml @19:39:02.38 delay 2 seconds @19:39:04.40 CSV.dial CTV @19:39:04.93 CS.seek 1.275ml @19:39:14.11 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @19:39:14.13 CH.seek 85C,8:00 @19:43:31.45 GoodNews.email "Starting 18tun w/pucks in tube 2\n{}\nCan@18:13:25, 20.9C, 23% humidity, 14.8psia, 11.996V, 0.664A, 0.664A avg, 7.96W",Subject: "18tun begun" @19:43:31.50 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:43:31.51 Retry #10 of 10 after 10:00 @19:44:45.91
CH.hold 5:00,85C @19:49:46.06 Collection Puck Heater at 84.1C after lysing @19:49:46.08 CS.seek 0ml @19:49:50.42 CTV.dial between spare and air,avoiding: :intake @19:49:51.63 CSV.dial :air @19:49:52.16 CS.seek 5ml @19:50:05.95 CSV.dial CTV @19:50:06.48 delay 2 seconds @19:50:08.50 CSV.dial :air @19:50:09.02 delay 2 seconds @19:50:11.04 CSV.dial CTV @19:50:11.57 delay 2 seconds @19:50:13.59 CSV.dial :air @19:50:14.12 delay 2 seconds @19:50:16.15 CS.seek 0ml @19:50:30.04 Recovering Lysate into Collection syringe @19:50:30.05 CSV.dial CBV @19:50:31.27 CBV.dial CSV @19:50:31.71 CS.seek 5ml @19:50:45.45 delay 5 seconds @19:50:50.47 CTV.dial :air @19:50:51.07 CS.seek 6.999ml @19:50:57.14 delay 5 seconds @19:51:02.16 CSV.dial :air2 @19:51:02.68 CS.seek 10ml @19:51:11.42 CSV.dial between air and CTV,avoiding: CBV @19:51:13.24 Lysing Complete @19:51:13.32 Storing sh1 puck in Collection Clamp to tube 1 @19:51:13.50 SC.reconfigure SCtight @19:51:13.56 SC.seek 1 @19:51:15.71 SC.reconfigure SCloose @19:51:15.93 Elbow.reconfigure TightElbow @19:51:15.99 Elbow.seek 1 @19:51:17.92 Forearm.reconfigure TightForearm @19:51:17.98 Forearm.seek 1 + 700 counts @19:51:19.03 SE.reconfigure SEpick @19:51:19.12 SE.seek beamBreak @19:51:22.10 SE.configure SEconfig @19:51:22.17 SE.seek :place @19:51:22.74 Forearm.reconfigure LooseForearm @19:51:22.80 Forearm.seek :retract @19:51:23.72 Elbow.seek CC @19:51:25.44 Elbow.reconfigure LooseElbow @19:51:25.54 Forearm.seek CC @19:51:26.61 Hand.close @19:51:27.36 CC.seek :open @19:51:31.82 Forearm.seek :retract @19:51:32.88 Elbow.reconfigure TightElbow @19:51:32.94 Elbow.seek 1 @19:51:34.91 Elbow.reconfigure LimpElbow @19:51:34.98 Forearm.seek 1 @19:51:36.03 Elbow.hold @19:51:36.11 Elbow.reconfigure TightElbow @19:51:36.17 Hand.open @19:51:36.98 Forearm.force -20 @19:51:37.03 Forearm.seek 1 + 346 counts @19:51:37.36 Forearm.reconfigure TightForearm @19:51:37.42 Forearm.seek 1 + 700 counts @19:51:37.71 SE.seek :down @19:51:38.84
Forearm.reconfigure LooseForearm @19:51:38.90 Forearm.seek :retract @19:51:40.56 SE.coast @19:51:40.61
SC.coast @19:51:40.67 Tranferring lysate to PRV @19:51:40.68 CSV.dial :air @19:51:41.04 CS.seek 10ml @19:51:41.14 CSV.dial between air and CTV @19:51:41.49 AIV.dial :bypass @19:51:41.51 Beginning samplePassC @19:51:41.53 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:51:41.62 Hand.open @19:51:41.99 Elbow.seek FlushPuck::Garage @19:51:44.24 Elbow.reconfigure LooseElbow @19:51:44.35 Forearm.seek :garage @19:51:45.41 Hand.close @19:51:46.23 Forearm.seek :retract @19:51:47.28 Elbow.reconfigure TightElbow @19:51:47.34 Elbow.seek CC @19:51:50.14 Elbow.reconfigure LooseElbow @19:51:50.24 Forearm.reconfigure TightForearm @19:51:50.30 Forearm.seek CC @19:51:51.63 CC.seek :closed @19:51:55.93 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.38 @19:51:56.02 Hand.open @19:51:56.80 Forearm.reconfigure LooseForearm @19:51:56.86 Forearm.seek :retract @19:51:57.91 AWV.select :air @19:51:57.92 AIV.dial :bypass @19:51:57.95 PSV.dial PSR @19:51:59.49 Pulling 1.5ml of PSR.flush PSR.select :flush @19:51:59.64 PS.seek 1.5ml @19:52:15.91 delay 5 seconds @19:52:20.93 PSV.dial :waste1 @19:52:21.47 PS.seek 0ml @19:52:37.69 PSV.dial PSR @19:52:38.21 PSR.select :air @19:52:38.34 PS.seek 2.5ml @19:53:05.01 PRV.dial CBV @19:53:05.04
CBV.dial PRV @19:53:05.98 PSV.dial :mixloop @19:53:07.15 CTV.dial :air,avoiding: :intake @19:53:07.16 PTV.dial :PRVmixing @19:53:08.02 PS.seek 0ml @19:53:31.54 GoodNews.email "Starting 18tun w/pucks in tube 2\n{}\nCan@18:13:25, 20.9C, 23% humidity, 14.8psia, 11.996V, 0.664A, 0.664A avg, 7.96W",Subject: "18tun begun" @19:53:31.58 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:53:31.60 GoodNews.email "Then, in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:13:30, 20.8C, 23% humidity, 14.8psia, 12.001V, 0.657A, 0.651A avg, 7.89W",Subject: "Asleep until 18:13" @19:53:31.64 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @19:53:31.66 Retry #1 of 10 after 10:00 @19:53:34.63
PSV.dial :air @19:53:35.16 PS.seek 2.5ml @19:54:01.82 PSV.dial :mixloop @19:54:02.34 PS.seek 0ml @19:54:28.91 PSV.dial :air @19:54:29.44 PS.seek 2.5ml @19:54:56.01 PSV.dial :mixloop @19:54:56.53 PS.seek 0ml @19:55:23.35 Garaging FlushPuck in Collection Clamp @19:55:23.44 Elbow.reconfigure TightElbow @19:55:23.50 Elbow.seek CC @19:55:23.72 Elbow.reconfigure LooseElbow @19:55:23.83 Forearm.seek CC @19:55:24.91 Hand.close @19:55:25.62 CC.seek :open @19:55:30.03 Forearm.seek :retract @19:55:31.53 Elbow.reconfigure TightElbow @19:55:31.62 Elbow.seek :garage @19:55:34.80 Elbow.reconfigure LooseElbow @19:55:34.90 Forearm.reconfigure TightForearm @19:55:34.97 Forearm.seek :garage @19:55:36.13 Hand.open @19:55:36.93 Forearm.reconfigure LooseForearm @19:55:36.99 Forearm.seek :retract @19:55:38.07 Connecting PS to CS to transfer sample @19:55:38.08 CBV.dial AIV @19:55:38.10 AIV.dial :bypass @19:55:38.12 PSV.dial :mixloop @19:55:38.14 PTV.dial :PRVmixing @19:55:38.16 PRV.dial AIV @19:55:38.19 CTV.dial :air,avoiding: :intake @19:55:38.21 Loading lyfil puck from tube 2 into Collection Clamp @19:55:38.45 SC.reconfigure SCtight @19:55:38.51 SC.seek 2 @19:55:40.62 SC.reconfigure SCloose @19:55:40.83 Elbow.reconfigure TightElbow @19:55:40.89 Elbow.seek 2 @19:55:43.03 Forearm.reconfigure TightForearm @19:55:43.09 Forearm.seek 2 + 700 counts @19:55:44.10 SE.reconfigure SEpick @19:55:44.19 SE.seek beamBreak @19:55:47.32 SE.configure SEconfig @19:55:47.48 Elbow.seek 2 @19:55:47.69 Forearm.seek 2 + 300 counts @19:55:47.93 Forearm.reconfigure LooseForearm @19:55:47.99 Hand.close @19:55:48.76 SE.seek :down @19:55:48.97
Forearm.seek :retract @19:55:49.91 Elbow.seek CC @19:55:51.55 Elbow.reconfigure LooseElbow @19:55:51.64 SE.coast @19:55:51.74
Forearm.reconfigure TightForearm @19:55:51.80 Forearm.seek CC @19:55:53.12 CC.seek :closed @19:55:57.38 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.42 @19:55:57.39 Hand.open @19:55:58.19 Forearm.reconfigure LooseForearm @19:55:58.25 Forearm.seek :retract @19:55:59.29 SC.coast @19:55:59.34 CTV.dial CSV,avoiding: :intake @19:56:00.89 CSV.dial CTV @19:56:01.24 CS.seek 5ml @19:56:14.98 delay 1:20 @19:57:35.02 CS.seek 2.5ml @19:57:42.55 delay 40 seconds @19:58:22.58 CS.seek 0ml @19:58:29.98 delay 1:00 @19:59:30.00 PSV.dial :air @19:59:30.50 PS.seek 2.5ml @19:59:57.13 PSV.dial :mixloop @19:59:57.63 delay 3 seconds @20:00:00.65 PSV.dial :air @20:00:01.60 delay 2 seconds @20:00:03.62 PSV.dial :mixloop @20:00:04.12 delay 3 seconds @20:00:07.14 PSV.dial :waste1 @20:00:08.63 PS.seek :empty @20:00:35.32 CBV.dial :waste1 @20:00:35.86 delay 5 seconds @20:00:40.88 CBV.dial AIV @20:00:41.42 delay 10 seconds @20:00:51.45 CBV.dial :waste1 @20:00:51.99 delay 10 seconds @20:01:02.02 CBV.dial AIV @20:01:02.56 delay 10 seconds @20:01:12.59 CTV.dial CSV,avoiding: :intake @20:01:12.60 CBV.dial between AIV and waste1 @20:01:12.99 CSV.dial :air @20:01:13.53 CS.seek 10ml @20:01:40.02 CSV.dial CTV @20:01:40.56 CS.seek 0ml @20:02:07.58 CBV.dial :waste1 @20:02:07.95 delay 10 seconds @20:02:17.98 CTV.dial :air,avoiding: :intake @20:02:19.58 Storing lyfil puck in Collection Clamp to tube 1 @20:02:19.75 SC.reconfigure SCtight @20:02:19.81 SC.seek 1 @20:02:21.92 SC.reconfigure SCloose @20:02:22.14 Elbow.reconfigure TightElbow @20:02:22.20 Elbow.seek 1 @20:02:24.17 Forearm.reconfigure TightForearm @20:02:24.23 Forearm.seek 1 + 700 counts @20:02:25.36 SE.reconfigure SEpick @20:02:25.45 SE.seek beamBreak @20:02:28.66 SE.configure SEconfig @20:02:28.82 Elbow.reconfigure LimpElbow @20:02:28.88 Forearm.reconfigure LooseForearm @20:02:28.94 Forearm.seek 1 @20:02:29.31 Elbow.hold @20:02:29.39 Elbow.reconfigure TightElbow @20:02:29.49 SE.seek 0.07 Pucks below Top Plate @20:02:30.08 Forearm.force -20 @20:02:30.13 Forearm.seek 1 + 326 counts @20:02:30.46 Forearm.reconfigure TightForearm @20:02:30.52 Forearm.seek 1 + 700 counts @20:02:30.76 SE.seek 1.07 Pucks below Top Plate @20:02:31.49 Forearm.reconfigure LooseForearm @20:02:31.54 Forearm.seek :retract @20:02:32.48 Elbow.seek CC @20:02:34.06 Elbow.reconfigure LooseElbow @20:02:34.16 Forearm.seek CC @20:02:35.23 Hand.close @20:02:36.00 CC.seek :open @20:02:40.49 Forearm.seek :retract @20:02:41.53 Elbow.reconfigure TightElbow @20:02:41.59 Elbow.seek 1 @20:02:43.58 Elbow.reconfigure LimpElbow @20:02:43.64 Forearm.seek 1 @20:02:45.05 Elbow.hold @20:02:45.20 Elbow.reconfigure TightElbow @20:02:45.26 Hand.open @20:02:46.12 Forearm.force -20 @20:02:46.17 Forearm.seek 1 + 347 counts @20:02:46.49 Forearm.reconfigure TightForearm @20:02:46.55 Forearm.seek 1 + 700 counts @20:02:46.94 SE.seek :down @20:02:48.07
Forearm.reconfigure LooseForearm @20:02:48.13 Forearm.seek :retract @20:02:49.76 SE.coast @20:02:49.80
SC.coast @20:02:49.85 PSV.dial :air @20:02:51.07 PS.seek 0.499ml @20:02:56.98 delay 3 seconds @20:03:00.00 PSV.dial :mixloop @20:03:00.48 PRV.dial :air @20:03:01.01 delay 3 seconds @20:03:04.03 PRV.dial CBV @20:03:04.56 CBV.dial AIV @20:03:05.11 PRV.dial between CBV and air @20:03:05.48 PRV.dial :air @20:03:05.88 PS.reconfigure PSslow @20:03:05.94 PS.seek 0.599ml @20:03:08.48 PRV.dial :waste2 @20:03:09.32 PS.reconfigure PSconfig @20:03:09.38 PS.seek :empty @20:03:16.35 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:03:16.43 Hand.open @20:03:16.75 Elbow.seek FlushPuck::Garage @20:03:18.81 Elbow.reconfigure LooseElbow @20:03:18.92 Forearm.seek :garage @20:03:19.97 Hand.close @20:03:20.87 Forearm.seek :retract @20:03:21.91 Elbow.reconfigure TightElbow @20:03:21.97 Elbow.seek CC @20:03:24.89 Elbow.reconfigure LooseElbow @20:03:24.99 Forearm.reconfigure TightForearm @20:03:25.05 Forearm.seek CC @20:03:26.40 CC.seek :closed @20:03:30.76 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.32 @20:03:30.84 Hand.open @20:03:31.69 Forearm.reconfigure LooseForearm @20:03:31.75 Forearm.seek :retract @20:03:31.81 GoodNews.email "Then, in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:13:30, 20.8C, 23% humidity, 14.8psia, 12.001V, 0.657A, 0.651A avg, 7.89W",Subject: "Asleep until 18:13" @20:03:31.84 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @20:03:31.87 Retry #2 of 10 after 10:00 @20:03:32.83
Clearing PML for modified lysate @20:03:32.84 CTV.dial :air,avoiding: :intake @20:03:32.86 PRV.dial :waste2 @20:03:32.88 PTV.dial :PRV,avoiding: :puck @20:03:34.20 delay 2 seconds @20:03:36.22 PTV.dial :PRVmixing,avoiding: :puck @20:03:37.07 delay 2 seconds @20:03:39.09 PSV.dial :PML @20:03:39.10 delay 2 seconds @20:03:41.14 PRV.dial :air @20:03:41.99 delay 2 seconds @20:03:44.07 PS.seek 1.499ml @20:04:00.38 delay 5 seconds @20:04:05.41 PS.seek 2.5ml @20:04:16.54 PSV.dial :air @20:04:17.05 PS.seek 0ml @20:04:43.60 mixing lysate with speDil @20:04:43.61 PSV.dial :PML @20:04:44.12 CBV.dial :PRV @20:04:44.13 PRV.dial :air @20:04:44.16 delay 2 seconds @20:04:46.19 PRV.dial :speDil @20:04:46.73 delay 2 seconds @20:04:48.81 PS.reconfigure PSslow1 @20:04:48.88 PS.seek 0.225ml @20:05:12.82 delay 5 seconds @20:05:17.84 PRV.dial CBV @20:05:18.71 delay 2 seconds @20:05:20.79 PS.seek 0.475ml @20:05:46.73 delay 5 seconds @20:05:51.75 PRV.dial :air @20:05:52.45 delay 2 seconds @20:05:54.48 PS.seek 0.965ml @20:06:44.64 delay 10 seconds @20:06:54.67 PRV.dial CBV @20:06:55.18 CTV.dial :air,avoiding: :intake @20:06:55.19 PSV.dial :air @20:06:55.70 PS.reconfigure PSconfig @20:06:55.76 PS.seek 2.5ml @20:07:12.20 delay 3 seconds @20:07:15.22 PSV.dial PTV @20:07:16.08 PTV.dial :PRV,avoiding: :puck @20:07:16.93 PS.seek 0ml @20:07:43.29 PSV.dial :air @20:07:44.15 PS.seek 2.5ml @20:08:10.48 delay 3 seconds @20:08:13.50 PSV.dial PTV @20:08:14.35 PTV.dial :PRV,avoiding: :puck @20:08:14.38 PS.seek 0ml @20:08:40.57 Pushing modified lysate into AIV Injection Loop @20:08:40.58 CBV.dial AIV @20:08:40.61 AIV.dial :bypass @20:08:40.63 PRV.dial CBV @20:08:40.66 PSV.dial :air @20:08:41.52 PS.seek 2.5ml @20:09:07.79 delay 3 seconds @20:09:10.81 PSV.dial :PML @20:09:11.32 PTV.dial :PRVmixing,avoiding: :puck @20:09:12.21 PS.reconfigure PSslow1 @20:09:12.27 PS.seek 1.74ml @20:10:29.67 delay 5 seconds @20:10:34.69 AIV.dial :sample @20:10:35.33 AWV.select :waste @20:10:35.50 PS.reconfigure PSslow2 @20:10:35.56 PS.seek 1.22ml @20:12:23.20 delay 15 seconds @20:12:38.22 AIV.dial :bypass @20:12:38.87 PS.reconfigure PSconfig @20:12:38.93 PS.seek 0ml @20:12:52.20 PSV.dial :air @20:12:52.71 PS.seek 2.5ml @20:13:19.07 PSV.dial :PML @20:13:19.58 PS.seek 0ml @20:13:31.89 GoodNews.email "Then, in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:13:30, 20.8C, 23% humidity, 14.8psia, 12.001V, 0.657A, 0.651A avg, 7.89W",Subject: "Asleep until 18:13" @20:13:31.94 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @20:13:31.95 Retry #3 of 10 after 10:00 @20:13:45.79
AIV.dial :coilToMFB @20:13:45.80 AWV.select :air @20:13:45.98 Extracting Nucleic Acid from AIV @20:13:46.02 SPE.hold 2:46:40,55C @20:13:46.05
Beginning samplePassD @20:13:46.58 ASV.dial :SPEdelivery @20:13:46.67
AWV.select :air @20:13:46.68 AIV.dial :bypass @20:13:46.71 PSV.dial PSR @20:13:47.02 delay 20 seconds @20:13:48.01
Pulling 1.5ml of PSR.killMFB @20:13:48.02 PSR.select :killMFB @20:13:48.17 PS.seek 1.5ml @20:14:05.07 delay 5 seconds @20:14:07.04 primeLysateINT @20:14:07.05 AWV.select :air @20:14:07.07 AIV.dial :coilToMFB @20:14:07.10 ATV.dial AIV @20:14:07.59 AS.reconfigure ASslow1 @20:14:07.65 AS.seek 0.3ml @20:14:10.09
PSV.dial :waste1 @20:14:10.65 PS.seek 0ml @20:14:27.61 PSV.dial PSR @20:14:28.15 PSR.select :air @20:14:28.28 PS.seek 2.5ml @20:14:56.20 PRV.dial CBV @20:14:56.23
CBV.dial PRV @20:14:56.25 PSV.dial :mixloop @20:14:57.48 CTV.dial :air,avoiding: :intake @20:14:57.49 PTV.dial :PRVmixing @20:14:57.53 PS.seek 0ml @20:15:08.34 delay 10 seconds @20:15:18.37 ATV.dial :air2 @20:15:19.28 AS.seek 0.33ml @20:15:25.33
PSV.dial PSR @20:15:25.63 ATV.dial :waste2 @20:15:26.25 AS.reconfigure ASnorm @20:15:26.31 AS.seek :empty @20:15:26.60
Pulling 1.5ml of PSR.flush @20:15:26.61 PSR.select :flush @20:15:26.76 PS.seek 1.5ml @20:15:43.38 Lysate primed! @20:15:43.39 AFV.select :holdingCoil @20:15:43.40 ATV.dial :air2 @20:15:43.86
delay 5 seconds @20:15:44.08 AS.reconfigure ASslow1 @20:15:44.14 AS.seek 0.069ml @20:15:48.88
PSV.dial :waste1 @20:15:49.43 PS.seek 0ml @20:15:58.56 delay 3 seconds @20:16:01.58 ATV.dial :AIV @20:16:02.51 AS.seek 0.469ml @20:16:06.39
PSV.dial PSR @20:16:06.93 PSR.select :air @20:16:07.06 PS.seek 2.5ml @20:16:34.73 PRV.dial CBV @20:16:34.76
CBV.dial PRV @20:16:34.78 PSV.dial :mixloop @20:16:35.98 CTV.dial :air,avoiding: :intake @20:16:35.99 PTV.dial :PRVmixing @20:16:36.03 PS.seek 0ml @20:17:03.67 PSV.dial PSR @20:17:04.92 Pulling 1.5ml of PSR.flush PSR.select :flush @20:17:05.08 PS.seek 1.5ml @20:17:21.76 delay 5 seconds @20:17:23.22 delay 5 seconds @20:17:26.78
PSV.dial :waste1 @20:17:27.31 PS.seek 0ml @20:17:28.29 Solid Phase Extraction Heater at 50.3C @20:17:28.30 ATV.dial ASV @20:17:28.72 ASV.dial :SPEdelivery @20:17:28.81 AS.seek 0.041ml @20:17:43.98
PSV.dial PSR @20:17:44.51 PSR.select :air @20:17:44.64 PS.seek 2.5ml @20:18:11.55 PRV.dial CBV @20:18:11.58
CBV.dial PRV @20:18:11.60 PSV.dial :mixloop @20:18:12.78 CTV.dial :air,avoiding: :intake @20:18:12.79 PTV.dial :PRVmixing @20:18:12.83 PS.seek 0ml @20:18:40.22 PSV.dial PSR @20:18:41.51 Pulling 1.5ml of PSR.flush @20:18:41.52 PSR.select :flush @20:18:41.67 PS.seek 1.5ml @20:18:55.16 delay 10 seconds @20:18:58.17
delay 5 seconds @20:19:03.19 PSV.dial :waste1 @20:19:03.73 PS.seek 0ml @20:19:05.18 rinseLysateLineINT @20:19:05.19 AFV.select :carrierSolution @20:19:05.31 delay 2 seconds @20:19:07.34 AS.reconfigure ASconfig @20:19:07.39 AS.seek 1ml @20:19:20.11
PSV.dial PSR @20:19:20.75 PSR.select :air @20:19:20.88 PS.seek 2.5ml @20:19:47.80 PRV.dial CBV @20:19:47.83
CBV.dial PRV @20:19:47.85 PSV.dial :mixloop @20:19:49.05 CTV.dial :air,avoiding: :intake @20:19:49.06 PTV.dial :PRVmixing @20:19:49.09 PS.reconfigure PSslow @20:19:49.17 PS.seek 1.2ml @20:20:02.80 delay 1 second @20:20:03.82 AFV.select :holdingCoil @20:20:03.92 delay 1 second @20:20:04.94 ATV.dial AIV @20:20:05.61 AIV.dial :coilToMFB @20:20:05.62 AWV.select :waste @20:20:05.75 AS.reconfigure ASslow0 @20:20:05.80 AS.seek 0ml @20:20:16.08
PSV.dial :waste1 @20:20:17.59 PS.reconfigure PSconfig @20:20:17.64 PS.seek 0ml @20:20:31.05 PSV.dial :air @20:20:32.23 PS.seek 2.5ml @20:20:58.76 PSV.dial :mixloop @20:20:59.27 PS.reconfigure PSslow @20:20:59.33 PS.seek 0ml @20:21:46.73 ATV.dial :air2 @20:21:47.42 AS.reconfigure ASslow1 @20:21:47.48 AS.seek 0.05ml @20:21:50.64
PSV.dial :air @20:21:51.17 PS.reconfigure PSconfig @20:21:51.22 PS.seek 2.5ml @20:21:57.83 ATV.dial :AIV @20:21:58.69 AS.seek 0.01ml @20:22:07.03 ATV.dial :waste @20:22:08.75 AS.seek :empty @20:22:11.23 ethanol wash @20:22:11.24 ATV.dial :air2 @20:22:12.57 AS.reconfigure ASslow2 @20:22:12.63 AS.seek 0.009ml @20:22:17.83
PSV.dial :mixloop @20:22:18.33 PS.reconfigure PSslow @20:22:18.39 PS.seek 0ml @20:22:23.04 delay 3 seconds @20:22:26.06 Pulling 0.4ml of Ethanol Wash @20:22:26.07 ATV.dial :ethanol @20:22:26.94 AS.reconfigure ASslow1 @20:22:27.00 AS.seek 0.409ml @20:23:09.55
Cleaning Collection Syringe @20:23:09.56 CSV.dial CSR @20:23:10.15 Pulling 4ml of CSR.flush CSR.select :flush @20:23:10.30 CS.seek 4ml @20:23:21.65 delay 5 seconds @20:23:26.67 CSR.select :air @20:23:26.81 CS.seek 10ml @20:23:31.97 GoodNews.email "Then, in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:13:30, 20.8C, 23% humidity, 14.8psia, 12.001V, 0.657A, 0.651A avg, 7.89W",Subject: "Asleep until 18:13" @20:23:32.03 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @20:23:32.04 Retry #4 of 10 after 10:00 @20:23:43.22 CSV.dial CTV @20:23:43.25
CTV.dial CSV @20:23:44.86 CBV.dial :waste1 @20:23:45.42 CS.seek 0ml @20:23:47.69 delay 3 seconds @20:23:50.71 ATV.dial :air1 @20:23:51.14 delay 3 seconds @20:23:54.21 AS.seek 0.434ml @20:23:59.55 delay 3 seconds @20:24:02.57 ATV.dial :ethanol @20:24:03.67 AS.seek 0.419ml @20:24:06.94 delay 3 seconds @20:24:09.96 ATV.dial ASV @20:24:11.33 AS.seek 0.092ml @20:24:12.13
CSV.dial CSR @20:24:12.73 Pulling 4ml of CSR.flush @20:24:12.74 CSR.select :flush @20:24:12.89 CS.seek 4ml @20:24:24.69 delay 5 seconds @20:24:29.71 CSR.select :air @20:24:29.85 CS.seek 10ml @20:24:46.28 CSV.dial CTV @20:24:46.31
CTV.dial CSV @20:24:46.86 CBV.dial :waste1 @20:24:46.88 CS.seek 0ml @20:25:13.65 Garaging FlushPuck in Collection Clamp @20:25:13.73 Elbow.reconfigure TightElbow @20:25:13.79 Elbow.seek CC @20:25:14.09 Elbow.reconfigure LooseElbow @20:25:14.20 Forearm.seek CC @20:25:15.28 Hand.close @20:25:16.03 CC.seek :open @20:25:17.36 delay 3 seconds @20:25:20.42
Forearm.seek :retract @20:25:20.48 ATV.dial :waste2 @20:25:21.27 AS.reconfigure ASconfig @20:25:21.33 AS.seek :empty @20:25:21.51
Elbow.reconfigure TightElbow @20:25:21.57 Elbow.seek :garage @20:25:24.65 Elbow.reconfigure LooseElbow @20:25:24.77 Forearm.reconfigure TightForearm @20:25:24.82 Forearm.seek :garage @20:25:25.99 Hand.open @20:25:26.84 Forearm.reconfigure LooseForearm @20:25:26.90 Forearm.seek :retract @20:25:26.98 ATV.dial :air2 @20:25:27.40 AS.reconfigure ASslow1 @20:25:27.45 AS.seek 1ml @20:25:27.93
Skipping Whole Cell Archive @20:25:27.94 Starting finalPurge @20:25:27.97 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:25:28.05 Hand.open @20:25:28.34 Elbow.reconfigure TightElbow @20:25:28.40 Elbow.seek FlushPuck::Garage @20:25:28.53 Elbow.reconfigure LooseElbow @20:25:28.64 Forearm.seek :garage @20:25:29.67 Hand.close @20:25:30.52 Forearm.seek :retract @20:25:31.56 Elbow.reconfigure TightElbow @20:25:31.62 Elbow.seek CC @20:25:34.45 Elbow.reconfigure LooseElbow @20:25:34.55 Forearm.reconfigure TightForearm @20:25:34.61 Forearm.seek CC @20:25:35.92 CC.seek :closed @20:25:40.81 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.36 @20:25:40.90 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[3C:27->20] @20:25:40.92 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[3C:27->20] @20:25:40.95 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[3C:27->20] @20:25:40.97 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[3C:27->20] @20:25:40.99 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[3C:27->20] @20:25:41.02 Hand.open @20:25:41.85 Forearm.reconfigure LooseForearm @20:25:41.91 Forearm.seek :retract @20:25:42.96 Flushing the Collection Valve::Manifold @20:25:43.10 CSV.dial CSR @20:25:43.70 Pulling 5ml of CSR.flush @20:25:43.71 CSR.select :flush @20:25:43.86 CS.seek 5ml @20:25:57.81 delay 5 seconds @20:26:02.83 CSR.select :air @20:26:03.01 CS.reconfigure CSfast @20:26:03.07 CS.seek 7.5ml @20:26:10.50 CSV.dial :waste @20:26:12.14 CS.reconfigure CSconfig @20:26:12.20 CS.seek 0ml @20:26:32.41 Flushing lower manifold's waste1 port @20:26:32.55 CSV.dial :flush @20:26:33.09 CS.reconfigure CSfast @20:26:33.15 CS.seek 5ml @20:26:47.06 delay 5 seconds @20:26:52.08 CSV.dial :air @20:26:52.66 CS.seek 7ml @20:26:58.84 CSV.dial CTV @20:26:58.87
CTV.dial CSV @20:26:59.39 CBV.dial :waste1 @20:26:59.41 CS.seek 0ml @20:27:18.34 Flushing lower manifold's waste2b port @20:27:18.48 CSV.dial :flush @20:27:19.37 CS.seek 5ml @20:27:33.34 delay 5 seconds @20:27:38.36 CSV.dial :air @20:27:38.95 CS.seek 7ml @20:27:45.10 CSV.dial CTV @20:27:45.13
CTV.dial CSV @20:27:45.66 CBV.dial :waste2b @20:27:46.56 CS.seek 0ml @20:28:05.44 Flushing lower manifold's waste1 port @20:28:05.45 CSV.dial CSR @20:28:06.05 Pulling 4ml of CSR.mfbkill @20:28:06.06 CSR.select :mfbkill @20:28:06.20 CS.reconfigure CSconfig @20:28:06.26 CS.seek 4ml @20:28:17.56 delay 5 seconds @20:28:22.58 CSR.select :air @20:28:22.72 CS.reconfigure CSfast @20:28:22.79 CS.seek 10ml @20:28:39.19 CSV.dial CTV @20:28:39.22
CTV.dial CSV @20:28:39.75 CBV.dial :waste1 @20:28:40.65 CS.seek 0ml @20:28:49.03 delay 5 seconds @20:28:54.05 ATV.dial ASV @20:28:55.09 ASV.dial :SPEdelivery @20:28:55.11 AS.seek 0.02ml @20:29:07.13
Flushing lower manifold's waste2b port @20:29:07.14 CSV.dial CSR @20:29:07.75 Pulling 4ml of CSR.mfbkill @20:29:07.76 CSR.select :mfbkill @20:29:07.90 CS.reconfigure CSconfig @20:29:07.96 CS.seek 4ml @20:29:19.22 delay 5 seconds @20:29:24.24 CSR.select :air @20:29:24.38 CS.reconfigure CSfast @20:29:24.45 CS.seek 10ml @20:29:40.85 CSV.dial CTV @20:29:40.88
CTV.dial CSV @20:29:41.44 CBV.dial :waste2b @20:29:42.34 CS.seek 0ml @20:30:08.85 Killing upper manifold @20:30:09.31 CSV.dial CSR @20:30:09.92 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @20:30:10.06 CS.reconfigure CSconfig @20:30:10.13 CS.seek 4ml @20:30:21.44 delay 5 seconds @20:30:26.46 CSR.select :air @20:30:26.59 CS.reconfigure CSfast @20:30:26.66 CS.seek 10ml @20:30:43.03 CSV.dial CBV @20:30:43.06
CBV.dial CSV @20:30:44.31 CTV.dial :air @20:30:45.89 CS.seek 0ml @20:31:12.41 delay 7 seconds @20:31:19.44 CS.seek 3ml @20:31:28.07 CSV.dial :waste @20:31:28.59 CS.seek 0ml @20:31:37.25 Flushing upper manifold @20:31:37.39 CSV.dial :flush @20:31:37.92 CS.seek 5ml @20:31:51.69 delay 5 seconds @20:31:56.71 CSV.dial :air @20:31:57.31 CS.seek 7ml @20:32:03.35 CSV.dial CBV @20:32:03.38
CBV.dial CSV @20:32:04.60 CTV.dial :air @20:32:04.62 CS.seek 0ml @20:32:12.50 delay 5 seconds @20:32:17.52 ATV.dial :air2 @20:32:18.59 AS.seek 0.52ml @20:32:23.51
delay 7 seconds @20:32:30.54 CS.seek 3ml @20:32:39.16 CSV.dial :waste @20:32:39.70 CS.seek 0ml @20:32:48.46 CSV.dial :flush @20:32:48.98 CS.seek 3ml @20:32:57.60 delay 5 seconds @20:33:02.62 CSV.dial :air @20:33:03.20 CS.seek 5ml @20:33:09.22 CSV.dial :air @20:33:09.24 CS.seek 0ml @20:33:22.97 delay 7 seconds @20:33:30.00 CS.seek 3ml @20:33:32.07 GoodNews.email "Then, in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:13:30, 20.8C, 23% humidity, 14.8psia, 12.001V, 0.657A, 0.651A avg, 7.89W",Subject: "Asleep until 18:13" @20:33:32.12 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @20:33:32.13 Retry #5 of 10 after 10:00 @20:33:38.60
CSV.dial :waste @20:33:39.48 CS.seek 0ml @20:33:48.13 Flushing lower manifold's waste1 port @20:33:48.27 CSV.dial :flush @20:33:48.81 CS.seek 5ml @20:33:59.45 delay 5 seconds @20:34:02.66
delay 5 seconds @20:34:04.47 ATV.dial ASV @20:34:05.52 ASV.dial :wasteR @20:34:05.90 AS.seek 0.3ml @20:34:07.68
CSV.dial :air @20:34:08.25 CS.seek 7ml @20:34:14.60 CSV.dial CTV @20:34:14.63
CTV.dial CSV @20:34:16.27 CBV.dial :waste1 @20:34:16.81 CS.seek 0ml @20:34:35.72 Flushing lower manifold's waste2b port @20:34:35.86 CSV.dial :flush @20:34:36.73 CS.seek 5ml @20:34:50.48 delay 20 seconds @20:34:50.50
delay 5 seconds @20:34:55.54 CSV.dial :air @20:34:56.13 CS.seek 7ml @20:35:02.26 CSV.dial CTV @20:35:02.28
CTV.dial CSV @20:35:02.82 CBV.dial :waste2b @20:35:04.20 CS.seek 0ml @20:35:10.52 AS.seek 0.07ml @20:35:23.04
Garaging FlushPuck in Collection Clamp @20:35:23.13 Elbow.reconfigure TightElbow @20:35:23.19 Elbow.seek CC @20:35:23.44 Elbow.reconfigure LooseElbow @20:35:23.55 Forearm.seek CC @20:35:24.58 Hand.close @20:35:25.30 CC.seek :open @20:35:29.69 Forearm.seek :retract @20:35:30.73 Elbow.reconfigure TightElbow @20:35:30.79 Elbow.seek :garage @20:35:34.10 Elbow.reconfigure LooseElbow @20:35:34.21 Forearm.reconfigure TightForearm @20:35:34.27 Forearm.seek :garage @20:35:35.44 Hand.open @20:35:36.22 Forearm.reconfigure LooseForearm @20:35:36.28 Forearm.seek :retract @20:35:37.35 Completed [coreNEG] begun 18:14:18 @20:35:37.37 Extending 18tun until completion of MFBPCR @20:35:57.05 delay 20 seconds @20:36:17.08 ATV.dial :waste2 @20:36:17.86 AS.reconfigure ASconfig @20:36:17.92 AS.seek :empty @20:36:22.31 Elution @20:36:22.32 ATV.dial :air1 @20:36:23.51 AS.reconfigure ASslow1 @20:36:23.57 AS.seek 0.049ml @20:36:33.97 delay 3 seconds @20:36:36.99 ATV.dial :ElutionWater @20:36:37.70 AS.seek 0.109ml @20:36:50.11 delay 3 seconds @20:36:53.13 ATV.dial ASV,via: :air2 @20:36:55.32 ASV.dial :SPEelution @20:36:56.02 AFV.select :carrierSolution @20:36:56.18 AS.reconfigure ASnorm @20:36:56.24 AS.seek 0.759ml @20:37:29.31 delay 3 seconds @20:37:32.33 AFV.select :holdingCoil @20:37:32.48 AS.reconfigure ASslow1 @20:37:32.54 AS.seek 0.474ml @20:38:30.11 delay 5 seconds @20:38:35.19 AS.reconfigure ASslow2 @20:38:35.25 AS.seek 0.439ml @20:39:10.52 delay 1:00 @20:40:10.55 pushing eluate to holding coil @20:40:10.63 AS.seek 0.279ml @20:42:51.92 delay 30 seconds @20:43:21.95 ATV.dial :waste,via: :air3 @20:43:23.73 AS.reconfigure ASconfig @20:43:23.79 AS.seek :empty @20:43:32.16 GoodNews.email "Then, in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:13:30, 20.8C, 23% humidity, 14.8psia, 12.001V, 0.657A, 0.651A avg, 7.89W",Subject: "Asleep until 18:13" @20:43:32.20 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @20:43:32.22 Retry #6 of 10 after 10:00 @20:43:40.16 Parking Bleach in LysateSource line and SPEcolumn @20:43:40.17 ATV.dial :air3 @20:43:41.04 AS.reconfigure ASslow1 @20:43:41.10 AS.seek 0.029ml @20:43:47.42 ATV.dial :ElutionWater @20:43:48.04 AS.seek 0.059ml @20:43:54.33 ATV.dial :air3 @20:43:54.95 AS.seek 0.069ml @20:43:57.28 delay 2 seconds @20:43:59.30 ATV.dial :bleach @20:43:59.79 AS.seek 0.769ml @20:46:20.88 delay 3 seconds @20:46:23.90 ATV.dial :air3 @20:46:24.47 delay 3 seconds @20:46:27.54 AS.seek 0.794ml @20:46:32.84 delay 3 seconds @20:46:35.86 ATV.dial :bleach @20:46:36.48 AS.seek 0.779ml @20:46:39.80 delay 3 seconds @20:46:42.82 ATV.dial :AIV @20:46:44.13 AS.reconfigure ASconfig @20:46:44.19 AS.seek 0.015ml @20:47:28.42 ATV.dial :waste @20:47:30.45 AS.seek :empty @20:47:31.69 ATV.dial :air3 @20:47:32.39 AS.reconfigure ASslow1 @20:47:32.45 AS.seek 0.009ml @20:47:34.84 delay 2 seconds @20:47:36.86 ATV.dial :bleach @20:47:37.34 AS.seek 0.579ml @20:49:32.25 delay 3 seconds @20:49:35.27 ATV.dial :air3 @20:49:35.84 delay 3 seconds @20:49:38.92 AS.seek 0.604ml @20:49:44.24 delay 3 seconds @20:49:47.26 ATV.dial :bleach @20:49:47.87 AS.seek 0.589ml @20:49:51.14 delay 3 seconds @20:49:54.16 ATV.dial :ASV @20:49:55.23 ASV.dial :wasteR @20:49:55.84 AS.seek 0ml @20:51:54.63 delay 1:00 @20:52:54.66 AFV.select :carrierSolution @20:52:54.76 delay 2 seconds @20:52:56.79 AS.reconfigure ASconfig @20:52:56.84 AS.seek 1ml @20:53:32.25 GoodNews.email "Then, in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:13:30, 20.8C, 23% humidity, 14.8psia, 12.001V, 0.657A, 0.651A avg, 7.89W",Subject: "Asleep until 18:13" @20:53:32.30 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @20:53:32.31 Retry #7 of 10 after 10:00 @20:53:54.59 delay 1 second @20:53:55.61 AFV.select :holdingCoil @20:53:55.71 delay 1 second @20:53:56.73 ATV.dial ASV @20:53:56.74 ASV.dial :wasteR @20:53:56.77 AS.reconfigure ASslow1 @20:53:56.83 AS.seek 0ml @20:57:18.22 delay 20 seconds @20:57:38.25 ATV.dial :air3 @20:57:39.81 AS.seek 0.59ml @20:59:38.77 delay 3 seconds @20:59:41.79 ATV.dial :ASV @20:59:43.25 ASV.dial :wasteR @20:59:43.34 AS.seek 0.09ml @21:01:24.19 delay 30 seconds @21:01:54.22 ATV.dial :extSrc @21:01:55.02 delay 3 seconds @21:01:58.04 ATV.dial :ElutionWater @21:01:59.20 AS.seek 0.12ml @21:02:05.55 ATV.dial :air3 @21:02:06.19 AS.seek 0.13ml @21:02:08.48 delay 2 seconds @21:02:10.50 ATV.dial :bleach @21:02:10.99 AS.seek 0.23ml @21:02:31.36 delay 3 seconds @21:02:34.38 ATV.dial :air3 @21:02:34.95 delay 3 seconds @21:02:38.02 AS.seek 0.255ml @21:02:43.34 delay 3 seconds @21:02:46.36 ATV.dial :bleach @21:02:47.00 AS.seek 0.24ml @21:02:50.31 delay 3 seconds @21:02:53.33 ATV.dial :ASV @21:02:54.42 ASV.dial :SPEdelivery @21:02:54.81 AFV.select :carrierSolution @21:02:54.92 delay 2 seconds @21:02:56.95 AS.reconfigure ASconfig @21:02:57.00 AS.seek 0.5ml @21:03:12.25 delay 1 second @21:03:13.27 AFV.select :holdingCoil @21:03:13.42 AS.reconfigure ASslow1 @21:03:13.48 AS.seek 0.17ml @21:03:32.34 GoodNews.email "Then, in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:13:30, 20.8C, 23% humidity, 14.8psia, 12.001V, 0.657A, 0.651A avg, 7.89W",Subject: "Asleep until 18:13" @21:03:32.39 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @21:03:32.40 Retry #8 of 10 after 10:00 @21:04:20.14 SPE.hold 2:46:40,95C @21:04:20.19 delay 3:00 @21:07:20.23 AFV.select :carrierSolution @21:07:20.34 delay 2 seconds @21:07:22.37 AS.reconfigure ASconfig @21:07:22.42 AS.seek 1ml @21:08:10.47 delay 1 second @21:08:11.49 AFV.select :holdingCoil @21:08:11.59 delay 1 second @21:08:12.61 ASV.dial :wasteD @21:08:13.70 AS.seek 0ml @21:09:11.45 Priming Eluate @21:09:11.46 AFV.select :carrierSolution @21:09:11.58 delay 2 seconds @21:09:13.61 AS.seek 0.5ml @21:09:42.66 delay 1 second @21:09:43.68 AFV.select :holdingCoil @21:09:43.79 ATV.dial ASV @21:09:43.80 ASV.dial :SPErecovery @21:09:44.42 Swish #1 of 2 @21:09:44.50 AS.reconfigure ASslow0 @21:09:44.56 AS.seek 0.6ml @21:09:55.01 AS.seek 0.5ml @21:10:05.38 Swish #2 of 2 @21:10:05.46 AS.seek 0.6ml @21:10:16.41 AS.seek 0.5ml @21:10:26.81 delay 3 seconds @21:10:29.88 AS.reconfigure ASslow1 @21:10:29.94 AS.seek 0.684ml @21:11:07.28 delay 3 seconds @21:11:10.30 Eluate primed @21:11:10.31 ATV.dial :waste2 @21:11:10.98 AS.reconfigure ASnorm @21:11:11.04 AS.seek 0ml @21:11:45.84 SPE.coast @21:11:45.92 ASV.dial :SPErecovery @21:11:45.94 Setting up PCR for IPc -- writing /var/log/esp/IPc19may1421h.pcr @21:11:45.98 Building PCR Slug from ASV with MM1 mix, PP1 primer @21:11:46.00 Priming with carrier @21:11:46.02 ATV.dial ARV @21:11:47.05 ARV.dial :carrier @21:11:47.43 AS.seek 0.5ml @21:12:12.95 AFV.select :carrierSolution @21:12:13.07 delay 2 seconds @21:12:15.10 AS.reconfigure ASconfig @21:12:15.16 AS.seek 1ml @21:12:44.20 delay 1 second @21:12:45.22 AFV.select :holdingCoil @21:12:45.32 delay 1 second @21:12:46.34 ATV.dial :waste @21:12:47.89 AS.reconfigure ASnorm @21:12:47.96 AS.seek :empty @21:13:32.42 GoodNews.email "Then, in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:13:30, 20.8C, 23% humidity, 14.8psia, 12.001V, 0.657A, 0.651A avg, 7.89W",Subject: "Asleep until 18:13" @21:13:32.46 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @21:13:32.48 Retry #9 of 10 after 10:00 @21:13:38.70 AFV.select :carrierSolution @21:13:38.81 delay 2 seconds @21:13:40.84 AS.reconfigure ASconfig @21:13:40.90 AS.seek 0.5ml @21:14:10.02 delay 1 second @21:14:11.04 AFV.select :holdingCoil @21:14:11.15 Building Slug @21:14:11.16 ATV.dial :ARV @21:14:12.86 delay 2 seconds @21:14:14.88 ARV.dial :air1 @21:14:15.29 delay 2 seconds @21:14:17.36 AS.reconfigure ASslow2 @21:14:17.43 AS.seek 0.506ml @21:14:24.02 delay 2 seconds @21:14:26.04 ARV.dial :MM1 @21:14:26.66 delay 2 seconds @21:14:28.69 Adding MM1 @21:14:28.77 AS.seek 0.524ml @21:14:47.19 delay 25 seconds @21:15:12.21 ARV.dial :PP1 @21:15:12.61 Adding PP1 @21:15:12.63 delay 2 seconds @21:15:14.70 AS.seek 0.53ml @21:15:21.09 delay 10 seconds @21:15:31.12 ARV.dial :air1 @21:15:31.50 adding ARV air @21:15:31.52 delay 2 seconds @21:15:33.59 AS.seek 0.536ml @21:15:39.99 delay 5 seconds @21:15:45.01 ARV.dial :carrier @21:15:45.42 delay 7 seconds @21:15:52.50 AS.seek 0.594ml @21:16:51.17 delay 5 seconds @21:16:56.19 ATV.dial :ASV @21:16:56.59 delay 2 seconds @21:16:58.66 AS.seek 0.6ml @21:17:05.08 delay 4 seconds @21:17:09.10 ATV.dial ARV @21:17:09.66 delay 2 seconds @21:17:11.68 ARV.dial :carrier @21:17:11.69 delay 2 seconds @21:17:13.77 AS.seek 0.65ml @21:18:04.24 delay 4 seconds @21:18:08.26 ATV.dial PCR @21:18:08.89 ASV.dial :waste1 @21:18:09.69 ARV.dial :air1 @21:18:10.09 Positioning Slug by 0.324ml @21:18:10.19 AS.seek 0.326ml @21:23:32.51 GoodNews.email "Then, in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:13:30, 20.8C, 23% humidity, 14.8psia, 12.001V, 0.657A, 0.651A avg, 7.89W",Subject: "Asleep until 18:13" @21:23:32.56 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @21:23:32.57 Retry #10 of 10 after 10:00 @21:23:36.31 delay 5 seconds @21:23:41.33 ATV.dial ARV @21:23:42.22 ASV.dial :PCRlock @21:23:42.59 delay 5 seconds @21:23:47.62 Running IPc ProfileIPc thermocycling @21:23:47.63 PCR.program ProfileIPc @21:23:47.78 ProfileIPc should complete in < 1:50:49 @21:23:47.80 PCR.run 2:23:31.25 @21:33:32.59 GoodNews.email "Then, in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:13:30, 20.8C, 23% humidity, 14.8psia, 12.001V, 0.657A, 0.651A avg, 7.89W",Subject: "Asleep until 18:13" @21:33:32.64 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @21:33:32.65 GoodNews.email "At 18:14:18 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:14:18, 20.9C, 23% humidity, 14.8psia, 12.055V, 0.437A, 0.430A avg, 5.26W",Subject: "Started 18tun phase coreNEG" @21:33:32.69 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @21:33:32.71 Retry #1 of 10 after 10:00 @21:43:32.74 GoodNews.email "At 18:14:18 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:14:18, 20.9C, 23% humidity, 14.8psia, 12.055V, 0.437A, 0.430A avg, 5.26W",Subject: "Started 18tun phase coreNEG" @21:43:32.78 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @21:43:32.80 Retry #2 of 10 after 10:00 @21:53:32.83 GoodNews.email "At 18:14:18 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:14:18, 20.9C, 23% humidity, 14.8psia, 12.055V, 0.437A, 0.430A avg, 5.26W",Subject: "Started 18tun phase coreNEG" @21:53:32.88 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @21:53:32.89 Retry #3 of 10 after 10:00 @22:03:32.91 GoodNews.email "At 18:14:18 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:14:18, 20.9C, 23% humidity, 14.8psia, 12.055V, 0.437A, 0.430A avg, 5.26W",Subject: "Started 18tun phase coreNEG" @22:03:32.95 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @22:03:32.98 Retry #4 of 10 after 10:00 @22:13:33.01 GoodNews.email "At 18:14:18 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:14:18, 20.9C, 23% humidity, 14.8psia, 12.055V, 0.437A, 0.430A avg, 5.26W",Subject: "Started 18tun phase coreNEG" @22:13:33.04 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @22:13:33.06 Retry #5 of 10 after 10:00 @22:23:33.09 GoodNews.email "At 18:14:18 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:14:18, 20.9C, 23% humidity, 14.8psia, 12.055V, 0.437A, 0.430A avg, 5.26W",Subject: "Started 18tun phase coreNEG" @22:23:33.13 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @22:23:33.15 Retry #6 of 10 after 10:00 @22:33:33.18 GoodNews.email "At 18:14:18 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:14:18, 20.9C, 23% humidity, 14.8psia, 12.055V, 0.437A, 0.430A avg, 5.26W",Subject: "Started 18tun phase coreNEG" @22:33:33.22 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @22:33:33.24 Retry #7 of 10 after 10:00 @22:43:33.27 GoodNews.email "At 18:14:18 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:14:18, 20.9C, 23% humidity, 14.8psia, 12.055V, 0.437A, 0.430A avg, 5.26W",Subject: "Started 18tun phase coreNEG" @22:43:33.31 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @22:43:33.33 Retry #8 of 10 after 10:00 @22:48:58.52 PCR.off @22:49:01.06 PCR for IPc completed -- closed /var/log/esp/IPc19may1421h.pcr @22:49:01.14 Cleaning PCR @22:49:01.15 AFV.select :carrierSolution @22:49:01.27 delay 2 seconds @22:49:03.30 AS.reconfigure ASconfig @22:49:03.35 AS.seek 0.5ml @22:49:13.67 delay 1 second @22:49:14.69 AFV.select :holdingCoil @22:49:14.79 ASV.dial :waste1 @22:49:15.17 ATV.dial :air3 @22:49:16.48 AS.reconfigure ASslow1 @22:49:16.54 AS.seek 0.51ml @22:49:18.95 delay 2 seconds @22:49:20.97 ATV.dial :bleach @22:49:21.59 AS.seek 0.81ml @22:50:22.31 delay 3 seconds @22:50:25.33 ATV.dial :air3 @22:50:25.89 delay 3 seconds @22:50:28.96 AS.seek 0.835ml @22:50:34.31 delay 3 seconds @22:50:37.33 ATV.dial :bleach @22:50:37.95 AS.seek 0.82ml @22:50:41.39 delay 3 seconds @22:50:44.41 ARV.dial :waste @22:50:45.03 delay 3 seconds @22:50:48.05 ATV.dial :ARV @22:50:48.97 AS.reconfigure ASconfig @22:50:49.03 AS.seek 0.47ml @22:51:09.47 AFV.select :carrierSolution @22:51:09.58 delay 2 seconds @22:51:11.61 AS.seek 1ml @22:51:42.36 delay 1 second @22:51:43.38 AFV.select :holdingCoil @22:51:43.48 delay 1 second @22:51:44.51 AS.reconfigure ASfast @22:51:44.56 AS.seek 0ml @22:52:11.97 AFV.select :carrierSolution @22:52:12.08 delay 2 seconds @22:52:14.11 AS.reconfigure ASconfig @22:52:14.17 AS.seek 1ml @22:53:11.94 delay 1 second @22:53:12.96 AFV.select :holdingCoil @22:53:13.06 delay 1 second @22:53:14.09 AS.reconfigure ASfast @22:53:14.14 AS.seek 0ml @22:53:33.36 GoodNews.email "At 18:14:18 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:14:18, 20.9C, 23% humidity, 14.8psia, 12.055V, 0.437A, 0.430A avg, 5.26W",Subject: "Started 18tun phase coreNEG" @22:53:33.40 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @22:53:33.85 Retry #9 of 10 after 10:00 @22:53:41.52 AFV.select :carrierSolution @22:53:41.63 delay 2 seconds @22:53:43.66 AS.reconfigure ASconfig @22:53:43.71 AS.seek 1ml @22:54:41.47 delay 1 second @22:54:42.49 AFV.select :holdingCoil @22:54:42.59 delay 1 second @22:54:43.63 AS.reconfigure ASfast @22:54:43.68 AS.seek 0ml @22:55:11.05 ARV.dial between carrier and air1 @22:55:11.55 ATV.dial :air3 @22:55:12.86 AS.reconfigure ASslow1 @22:55:12.92 AS.seek 0.01ml @22:55:15.22 delay 2 seconds @22:55:17.24 ATV.dial :bleach @22:55:17.86 AS.seek 0.81ml @22:57:59.02 delay 3 seconds @22:58:02.04 ATV.dial :air3 @22:58:02.60 delay 3 seconds @22:58:05.67 AS.seek 0.835ml @22:58:11.03 delay 3 seconds @22:58:14.05 ATV.dial :bleach @22:58:14.69 AS.seek 0.82ml @22:58:18.00 delay 3 seconds @22:58:21.02 ATV.dial PCR @22:58:21.44 AS.reconfigure ASconfig @22:58:21.50 AS.seek :empty @22:59:08.97 PCR.program :bleachProfile @22:59:09.03 PCR program should complete in < 2:45 @22:59:09.04 PCR.run 10:00 @23:02:04.79 PCR.off @23:02:04.82 AFV.select :carrierSolution @23:02:04.93 delay 2 seconds @23:02:06.96 AS.seek 1ml @23:03:04.77 delay 1 second @23:03:05.79 AFV.select :holdingCoil @23:03:05.89 delay 1 second @23:03:06.92 AS.reconfigure ASfast @23:03:06.97 AS.seek 0ml @23:03:33.92 GoodNews.email "At 18:14:18 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:14:18, 20.9C, 23% humidity, 14.8psia, 12.055V, 0.437A, 0.430A avg, 5.26W",Subject: "Started 18tun phase coreNEG" @23:03:33.96 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @23:03:33.98 Retry #10 of 10 after 10:00 @23:03:34.34 delay 3 seconds @23:03:37.36 AFV.select :carrierSolution @23:03:37.46 delay 2 seconds @23:03:39.49 AS.reconfigure ASconfig @23:03:39.55 AS.seek 1ml @23:04:37.32 delay 1 second @23:04:38.34 AFV.select :holdingCoil @23:04:38.44 delay 1 second @23:04:39.47 AS.reconfigure ASfast @23:04:39.52 AS.seek 0ml @23:05:06.86 delay 3 seconds @23:05:09.88 AFV.select :carrierSolution @23:05:09.98 delay 2 seconds @23:05:12.01 AS.reconfigure ASconfig @23:05:12.07 AS.seek 1ml @23:06:09.85 delay 1 second @23:06:10.87 AFV.select :holdingCoil @23:06:10.97 delay 1 second @23:06:12.00 AS.reconfigure ASfast @23:06:12.05 AS.seek 0ml @23:06:39.39 delay 3 seconds @23:06:42.41 AFV.select :carrierSolution @23:06:42.51 delay 2 seconds @23:06:44.54 AS.reconfigure ASconfig @23:06:44.60 AS.seek 1ml @23:07:42.35 delay 1 second @23:07:43.37 AFV.select :holdingCoil @23:07:43.47 delay 1 second @23:07:44.50 AS.reconfigure ASfast @23:07:44.55 AS.seek 0ml @23:08:11.89 delay 3 seconds @23:08:14.91 AFV.select :carrierSolution @23:08:15.01 delay 2 seconds @23:08:17.04 AS.reconfigure ASconfig @23:08:17.10 AS.seek 1ml @23:09:14.91 delay 1 second @23:09:15.93 AFV.select :holdingCoil @23:09:16.03 delay 1 second @23:09:17.06 AS.reconfigure ASnorm @23:09:17.11 AS.seek 0ml @23:10:07.84 Setting up PCR for Pp -- writing /var/log/esp/Pp19may1423h.pcr @23:10:07.86 Building PCR Slug from ASV with MM1 mix, PP2 primer @23:10:07.88 Priming with carrier @23:10:07.90 ATV.dial ARV @23:10:08.53 ARV.dial :carrier @23:10:08.86 AS.seek 0.5ml @23:10:34.46 AFV.select :carrierSolution @23:10:34.58 delay 2 seconds @23:10:36.61 AS.reconfigure ASconfig @23:10:36.67 AS.seek 1ml @23:11:05.71 delay 1 second @23:11:06.73 AFV.select :holdingCoil @23:11:06.83 delay 1 second @23:11:07.85 ATV.dial :waste @23:11:09.55 AS.reconfigure ASnorm @23:11:09.61 AS.seek :empty @23:12:00.88 AFV.select :carrierSolution @23:12:00.99 delay 2 seconds @23:12:03.02 AS.reconfigure ASconfig @23:12:03.08 AS.seek 0.5ml @23:12:32.21 delay 1 second @23:12:33.23 AFV.select :holdingCoil @23:12:33.34 Building Slug @23:12:33.35 ATV.dial :ARV @23:12:35.03 delay 2 seconds @23:12:37.05 ARV.dial :air1 @23:12:37.45 delay 2 seconds @23:12:39.52 AS.reconfigure ASslow2 @23:12:39.59 AS.seek 0.506ml @23:12:45.86 delay 2 seconds @23:12:47.88 ARV.dial :MM1 @23:12:48.49 delay 2 seconds @23:12:50.52 Adding MM1 @23:12:50.60 AS.seek 0.524ml @23:13:09.25 delay 25 seconds @23:13:34.01 GoodNews.email "At 18:14:18 began in tube 2: coreNEG\n/home/esp/esp2/mission/18tun.rb:109\n{}\nCan@18:14:18, 20.9C, 23% humidity, 14.8psia, 12.055V, 0.437A, 0.430A avg, 5.26W",Subject: "Started 18tun phase coreNEG" @23:13:34.06 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @23:13:34.07 BACfans.email "Started mfb in tube 2 at 19:30:18CEST14-May-19\n{}\nCan@19:23:28, 21.1C, 24% humidity, 14.8psia, 12.055V, 0.462A, 0.462A avg, 5.57W",Subject: "pcrBAC" @23:13:34.11 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @23:13:34.14 Retry #1 of 10 after 10:00 @23:13:34.27 ARV.dial :PP2 @23:13:34.68 Adding PP2 @23:13:34.70 delay 2 seconds @23:13:36.77 AS.seek 0.53ml @23:13:43.25 delay 10 seconds @23:13:53.28 ARV.dial :air2 @23:13:53.66 adding ARV air @23:13:53.68 delay 2 seconds @23:13:55.76 AS.seek 0.536ml @23:14:02.21 delay 5 seconds @23:14:07.23 ARV.dial :carrier @23:14:08.50 delay 7 seconds @23:14:15.58 AS.seek 0.594ml @23:15:14.21 delay 5 seconds @23:15:19.23 ATV.dial :ASV @23:15:19.64 delay 2 seconds @23:15:21.71 AS.seek 0.6ml @23:15:28.14 delay 4 seconds @23:15:32.16 ATV.dial ARV @23:15:32.72 delay 2 seconds @23:15:34.74 ARV.dial :carrier @23:15:34.75 delay 2 seconds @23:15:36.83 AS.seek 0.65ml @23:16:27.47 delay 4 seconds @23:16:31.49 ATV.dial PCR @23:16:32.13 ASV.dial :waste1 @23:16:32.14 ARV.dial :air1 @23:16:32.66 Positioning Slug by 0.324ml @23:16:32.75 AS.seek 0.326ml @23:21:59.17 delay 5 seconds @23:22:04.19 ATV.dial ARV @23:22:05.08 ASV.dial :PCRlock @23:22:05.47 delay 5 seconds @23:22:10.50 Running Pp Profile60C350 thermocycling @23:22:10.51 PCR.program Profile60C350 @23:22:10.65 Profile60C350 should complete in < 1:48:05 @23:22:10.67 PCR.run 2:20:06.25 @23:23:34.17 BACfans.email "Started mfb in tube 2 at 19:30:18CEST14-May-19\n{}\nCan@19:23:28, 21.1C, 24% humidity, 14.8psia, 12.055V, 0.462A, 0.462A avg, 5.57W",Subject: "pcrBAC" @23:23:34.22 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @23:23:34.24 Retry #2 of 10 after 10:00 @23:33:34.27 BACfans.email "Started mfb in tube 2 at 19:30:18CEST14-May-19\n{}\nCan@19:23:28, 21.1C, 24% humidity, 14.8psia, 12.055V, 0.462A, 0.462A avg, 5.57W",Subject: "pcrBAC" @23:33:34.31 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @23:33:34.34 Retry #3 of 10 after 10:00 @23:43:34.37 BACfans.email "Started mfb in tube 2 at 19:30:18CEST14-May-19\n{}\nCan@19:23:28, 21.1C, 24% humidity, 14.8psia, 12.055V, 0.462A, 0.462A avg, 5.57W",Subject: "pcrBAC" @23:43:34.42 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @23:43:34.44 Retry #4 of 10 after 10:00 @23:53:34.47 BACfans.email "Started mfb in tube 2 at 19:30:18CEST14-May-19\n{}\nCan@19:23:28, 21.1C, 24% humidity, 14.8psia, 12.055V, 0.462A, 0.462A avg, 5.57W",Subject: "pcrBAC" @23:53:34.51 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @23:53:34.54 Retry #5 of 10 after 10:00 @00:03:34.56CEST15-May-19 BACfans.email "Started mfb in tube 2 at 19:30:18CEST14-May-19\n{}\nCan@19:23:28, 21.1C, 24% humidity, 14.8psia, 12.055V, 0.462A, 0.462A avg, 5.57W",Subject: "pcrBAC" @00:03:34.60 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @00:03:34.62 Retry #6 of 10 after 10:00 @00:13:34.65 BACfans.email "Started mfb in tube 2 at 19:30:18CEST14-May-19\n{}\nCan@19:23:28, 21.1C, 24% humidity, 14.8psia, 12.055V, 0.462A, 0.462A avg, 5.57W",Subject: "pcrBAC" @00:13:34.69 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @00:13:34.71 Retry #7 of 10 after 10:00 @00:23:34.74 BACfans.email "Started mfb in tube 2 at 19:30:18CEST14-May-19\n{}\nCan@19:23:28, 21.1C, 24% humidity, 14.8psia, 12.055V, 0.462A, 0.462A avg, 5.57W",Subject: "pcrBAC" @00:23:34.78 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @00:23:34.80 Retry #8 of 10 after 10:00 @00:33:34.83 BACfans.email "Started mfb in tube 2 at 19:30:18CEST14-May-19\n{}\nCan@19:23:28, 21.1C, 24% humidity, 14.8psia, 12.055V, 0.462A, 0.462A avg, 5.57W",Subject: "pcrBAC" @00:33:34.87 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @00:33:34.89 Retry #9 of 10 after 10:00 @00:43:34.92 BACfans.email "Started mfb in tube 2 at 19:30:18CEST14-May-19\n{}\nCan@19:23:28, 21.1C, 24% humidity, 14.8psia, 12.055V, 0.462A, 0.462A avg, 5.57W",Subject: "pcrBAC" @00:43:34.96 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @00:43:34.98 Retry #10 of 10 after 10:00 @00:45:27.45 PCR.off @00:45:29.45 PCR for Pp completed -- closed /var/log/esp/Pp19may1423h.pcr @00:45:29.52 Cleaning PCR @00:45:29.53 AFV.select :carrierSolution @00:45:30.16 delay 2 seconds @00:45:32.19 AS.reconfigure ASconfig @00:45:32.24 AS.seek 0.5ml @00:45:42.54 delay 1 second @00:45:43.56 AFV.select :holdingCoil @00:45:43.66 ASV.dial :waste1 @00:45:44.05 ATV.dial :air3 @00:45:45.36 AS.reconfigure ASslow1 @00:45:45.42 AS.seek 0.51ml @00:45:47.70 delay 2 seconds @00:45:49.72 ATV.dial :bleach @00:45:50.36 AS.seek 0.81ml @00:46:50.96 delay 3 seconds @00:46:53.98 ATV.dial :air3 @00:46:54.55 delay 3 seconds @00:46:57.62 AS.seek 0.835ml @00:47:02.94 delay 3 seconds @00:47:05.96 ATV.dial :bleach @00:47:06.60 AS.seek 0.82ml @00:47:09.93 delay 3 seconds @00:47:12.95 ARV.dial :waste @00:47:13.55 delay 3 seconds @00:47:16.57 ATV.dial :ARV @00:47:17.49 AS.reconfigure ASconfig @00:47:17.55 AS.seek 0.47ml @00:47:37.97 AFV.select :carrierSolution @00:47:38.08 delay 2 seconds @00:47:40.11 AS.seek 1ml @00:48:10.85 delay 1 second @00:48:11.87 AFV.select :holdingCoil @00:48:11.97 delay 1 second @00:48:13.00 AS.reconfigure ASfast @00:48:13.05 AS.seek 0ml @00:48:40.40 AFV.select :carrierSolution @00:48:40.51 delay 2 seconds @00:48:42.54 AS.reconfigure ASconfig @00:48:42.59 AS.seek 1ml @00:49:40.36 delay 1 second @00:49:41.38 AFV.select :holdingCoil @00:49:41.48 delay 1 second @00:49:42.51 AS.reconfigure ASfast @00:49:42.56 AS.seek 0ml @00:50:10.19 AFV.select :carrierSolution @00:50:10.30 delay 2 seconds @00:50:12.33 AS.reconfigure ASconfig @00:50:12.38 AS.seek 1ml @00:51:10.13 delay 1 second @00:51:11.15 AFV.select :holdingCoil @00:51:11.25 delay 1 second @00:51:12.28 AS.reconfigure ASfast @00:51:12.33 AS.seek 0ml @00:51:39.69 ARV.dial between carrier and air1 @00:51:40.19 ATV.dial :air3 @00:51:41.50 AS.reconfigure ASslow1 @00:51:41.56 AS.seek 0.01ml @00:51:43.93 delay 2 seconds @00:51:45.95 ATV.dial :bleach @00:51:46.58 AS.seek 0.81ml @00:53:35.01 BACfans.email "Started mfb in tube 2 at 19:30:18CEST14-May-19\n{}\nCan@19:23:28, 21.1C, 24% humidity, 14.8psia, 12.055V, 0.462A, 0.462A avg, 5.57W",Subject: "pcrBAC" @00:53:35.06 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @00:54:27.74 delay 3 seconds @00:54:30.76 ATV.dial :air3 @00:54:31.33 delay 3 seconds @00:54:34.40 AS.seek 0.835ml @00:54:39.71 delay 3 seconds @00:54:42.73 ATV.dial :bleach @00:54:43.36 AS.seek 0.82ml @00:54:46.69 delay 3 seconds @00:54:49.71 ATV.dial PCR @00:54:50.13 AS.reconfigure ASconfig @00:54:50.19 AS.seek :empty @00:55:37.68 PCR.program :bleachProfile @00:55:37.73 PCR program should complete in < 2:45 @00:55:37.74 PCR.run 10:00 @00:58:33.51 PCR.off @00:58:33.54 AFV.select :carrierSolution @00:58:33.65 delay 2 seconds @00:58:35.68 AS.seek 1ml @00:59:33.51 delay 1 second @00:59:34.53 AFV.select :holdingCoil @00:59:34.63 delay 1 second @00:59:35.66 AS.reconfigure ASfast @00:59:35.71 AS.seek 0ml @01:00:03.07 delay 3 seconds @01:00:06.09 AFV.select :carrierSolution @01:00:06.19 delay 2 seconds @01:00:08.22 AS.reconfigure ASconfig @01:00:08.28 AS.seek 1ml @01:01:06.10 delay 1 second @01:01:07.12 AFV.select :holdingCoil @01:01:07.75 delay 1 second @01:01:08.78 AS.reconfigure ASfast @01:01:08.83 AS.seek 0ml @01:01:36.21 delay 3 seconds @01:01:39.23 AFV.select :carrierSolution @01:01:39.34 delay 2 seconds @01:01:41.37 AS.reconfigure ASconfig @01:01:41.43 AS.seek 1ml @01:02:39.22 delay 1 second @01:02:40.24 AFV.select :holdingCoil @01:02:40.34 delay 1 second @01:02:41.37 AS.reconfigure ASfast @01:02:41.43 AS.seek 0ml @01:03:08.80 delay 3 seconds @01:03:11.82 AFV.select :carrierSolution @01:03:11.92 delay 2 seconds @01:03:13.95 AS.reconfigure ASconfig @01:03:14.01 AS.seek 1ml @01:04:11.79 delay 1 second @01:04:12.81 AFV.select :holdingCoil @01:04:12.91 delay 1 second @01:04:13.94 AS.reconfigure ASfast @01:04:13.99 AS.seek 0ml @01:04:41.37 delay 3 seconds @01:04:44.39 AFV.select :carrierSolution @01:04:44.49 delay 2 seconds @01:04:46.52 AS.reconfigure ASconfig @01:04:46.58 AS.seek 1ml @01:05:44.35 delay 1 second @01:05:45.37 AFV.select :holdingCoil @01:05:45.47 delay 1 second @01:05:46.50 AS.reconfigure ASnorm @01:05:46.55 AS.seek 0ml @01:06:37.29 Setting up PCR for Ss -- writing /var/log/esp/Ss19may1501h.pcr @01:06:37.31 Building PCR Slug from ASV with MM2 mix, PP4 primer @01:06:37.33 Priming with carrier @01:06:37.35 ATV.dial ARV @01:06:37.99 ARV.dial :carrier @01:06:38.35 AS.seek 0.5ml @01:07:03.91 AFV.select :carrierSolution @01:07:04.03 delay 2 seconds @01:07:06.06 AS.reconfigure ASconfig @01:07:06.12 AS.seek 1ml @01:07:35.21 delay 1 second @01:07:36.23 AFV.select :holdingCoil @01:07:36.33 delay 1 second @01:07:37.35 ATV.dial :waste @01:07:39.07 AS.reconfigure ASnorm @01:07:39.14 AS.seek :empty @01:08:29.91 AFV.select :carrierSolution @01:08:30.02 delay 2 seconds @01:08:32.05 AS.reconfigure ASconfig @01:08:32.11 AS.seek 0.5ml @01:09:01.18 delay 1 second @01:09:02.20 AFV.select :holdingCoil @01:09:02.31 Building Slug @01:09:02.32 ATV.dial :ARV @01:09:03.99 delay 2 seconds @01:09:06.01 ARV.dial :air1 @01:09:06.42 delay 2 seconds @01:09:08.49 AS.reconfigure ASslow2 @01:09:08.56 AS.seek 0.506ml @01:09:14.80 delay 2 seconds @01:09:16.82 ARV.dial :MM2 @01:09:18.32 delay 2 seconds @01:09:20.34 Adding MM2 @01:09:20.43 AS.seek 0.524ml @01:09:38.74 delay 25 seconds @01:10:03.76 ARV.dial :PP4 @01:10:04.16 Adding PP4 @01:10:04.18 delay 2 seconds @01:10:06.25 AS.seek 0.53ml @01:10:12.76 delay 10 seconds @01:10:22.79 ARV.dial :air3 @01:10:23.18 adding ARV air @01:10:23.19 delay 2 seconds @01:10:25.27 AS.seek 0.536ml @01:10:31.57 delay 5 seconds @01:10:36.59 ARV.dial :carrier @01:10:37.88 delay 7 seconds @01:10:44.97 AS.seek 0.594ml @01:11:43.43 delay 5 seconds @01:11:48.45 ATV.dial :ASV @01:11:48.87 delay 2 seconds @01:11:50.94 AS.seek 0.6ml @01:11:57.22 delay 4 seconds @01:12:01.24 ATV.dial ARV @01:12:01.80 delay 2 seconds @01:12:03.82 ARV.dial :carrier @01:12:03.83 delay 2 seconds @01:12:05.91 AS.seek 0.65ml @01:12:56.40 delay 4 seconds @01:13:00.42 ATV.dial PCR @01:13:01.05 ASV.dial :waste1 @01:13:01.06 ARV.dial :air1 @01:13:01.47 Positioning Slug by 0.324ml @01:13:01.56 AS.seek 0.326ml @01:18:27.83 delay 5 seconds @01:18:32.85 ATV.dial ARV @01:18:33.63 ASV.dial :PCRlock @01:18:34.54 delay 5 seconds @01:18:39.56 Running Ss Profile60C350 thermocycling @01:18:39.58 PCR.program Profile60C350 @01:18:39.72 Profile60C350 should complete in < 1:48:05 @01:18:39.74 PCR.run 2:20:06.25 @02:41:50.48 PCR.off @02:41:52.98 PCR for Ss completed -- closed /var/log/esp/Ss19may1501h.pcr @02:41:53.06 Cleaning PCR @02:41:53.07 AFV.select :carrierSolution @02:41:53.19 delay 2 seconds @02:41:55.22 AS.reconfigure ASconfig @02:41:55.27 AS.seek 0.5ml @02:42:05.56 delay 1 second @02:42:06.58 AFV.select :holdingCoil @02:42:06.68 ASV.dial :waste1 @02:42:07.07 ATV.dial :air3 @02:42:08.40 AS.reconfigure ASslow1 @02:42:08.46 AS.seek 0.51ml @02:42:10.75 delay 2 seconds @02:42:12.77 ATV.dial :bleach @02:42:13.41 AS.seek 0.81ml @02:43:14.01 delay 3 seconds @02:43:17.03 ATV.dial :air3 @02:43:17.59 delay 3 seconds @02:43:20.66 AS.seek 0.835ml @02:43:25.97 delay 3 seconds @02:43:28.99 ATV.dial :bleach @02:43:29.62 AS.seek 0.82ml @02:43:32.96 delay 3 seconds @02:43:35.98 ARV.dial :waste @02:43:36.60 delay 3 seconds @02:43:39.62 ATV.dial :ARV @02:43:40.54 AS.reconfigure ASconfig @02:43:40.60 AS.seek 0.47ml @02:44:01.02 AFV.select :carrierSolution @02:44:01.13 delay 2 seconds @02:44:03.16 AS.seek 1ml @02:44:33.93 delay 1 second @02:44:34.95 AFV.select :holdingCoil @02:44:35.05 delay 1 second @02:44:36.08 AS.reconfigure ASfast @02:44:36.13 AS.seek 0ml @02:45:03.48 AFV.select :carrierSolution @02:45:03.59 delay 2 seconds @02:45:05.62 AS.reconfigure ASconfig @02:45:05.67 AS.seek 1ml @02:46:03.48 delay 1 second @02:46:04.50 AFV.select :holdingCoil @02:46:04.60 delay 1 second @02:46:05.63 AS.reconfigure ASfast @02:46:05.68 AS.seek 0ml @02:46:33.04 AFV.select :carrierSolution @02:46:33.15 delay 2 seconds @02:46:35.19 AS.reconfigure ASconfig @02:46:35.24 AS.seek 1ml @02:47:32.99 delay 1 second @02:47:34.01 AFV.select :holdingCoil @02:47:34.11 delay 1 second @02:47:35.14 AS.reconfigure ASfast @02:47:35.19 AS.seek 0ml @02:48:02.79 ARV.dial between carrier and air1 @02:48:03.31 ATV.dial :air3 @02:48:04.63 AS.reconfigure ASslow1 @02:48:04.69 AS.seek 0.01ml @02:48:07.01 delay 2 seconds @02:48:09.03 ATV.dial :bleach @02:48:09.69 AS.seek 0.81ml @02:50:50.85 delay 3 seconds @02:50:53.87 ATV.dial :air3 @02:50:54.43 delay 3 seconds @02:50:57.50 AS.seek 0.835ml @02:51:02.81 delay 3 seconds @02:51:05.83 ATV.dial :bleach @02:51:06.46 AS.seek 0.82ml @02:51:09.79 delay 3 seconds @02:51:12.81 ATV.dial PCR @02:51:13.23 AS.reconfigure ASconfig @02:51:13.29 AS.seek :empty @02:52:00.78 PCR.program :bleachProfile @02:52:00.83 PCR program should complete in < 2:45 @02:52:00.84 PCR.run 10:00 @02:54:56.60 PCR.off @02:54:56.63 AFV.select :carrierSolution @02:54:56.75 delay 2 seconds @02:54:58.78 AS.seek 1ml @02:55:56.63 delay 1 second @02:55:57.65 AFV.select :holdingCoil @02:55:57.75 delay 1 second @02:55:58.78 AS.reconfigure ASfast @02:55:58.83 AS.seek 0ml @02:56:26.71 delay 3 seconds @02:56:29.73 AFV.select :carrierSolution @02:56:29.83 delay 2 seconds @02:56:31.86 AS.reconfigure ASconfig @02:56:31.92 AS.seek 1ml @02:57:29.70 delay 1 second @02:57:30.72 AFV.select :holdingCoil @02:57:30.82 delay 1 second @02:57:31.85 AS.reconfigure ASfast @02:57:31.90 AS.seek 0ml @02:57:59.31 delay 3 seconds @02:58:02.33 AFV.select :carrierSolution @02:58:02.43 delay 2 seconds @02:58:04.46 AS.reconfigure ASconfig @02:58:04.51 AS.seek 1ml @02:59:02.28 delay 1 second @02:59:03.30 AFV.select :holdingCoil @02:59:03.40 delay 1 second @02:59:04.43 AS.reconfigure ASfast @02:59:04.48 AS.seek 0ml @02:59:31.85 delay 3 seconds @02:59:34.87 AFV.select :carrierSolution @02:59:34.97 delay 2 seconds @02:59:37.00 AS.reconfigure ASconfig @02:59:37.06 AS.seek 1ml @03:00:34.84 delay 1 second @03:00:35.86 AFV.select :holdingCoil @03:00:35.96 delay 1 second @03:00:36.99 AS.reconfigure ASfast @03:00:37.04 AS.seek 0ml @03:01:04.38 delay 3 seconds @03:01:07.40 AFV.select :carrierSolution @03:01:07.50 delay 2 seconds @03:01:09.53 AS.reconfigure ASconfig @03:01:09.58 AS.seek 1ml @03:02:07.37 delay 1 second @03:02:08.39 AFV.select :holdingCoil @03:02:08.49 delay 1 second @03:02:09.52 AS.reconfigure ASnorm @03:02:09.57 AS.seek 0ml @03:03:00.31 Setting up PCR for Mm -- writing /var/log/esp/Mm19may1503h.pcr @03:03:00.33 Building PCR Slug from ASV with MM2 mix, PP3 primer @03:03:00.35 Priming with carrier @03:03:00.37 ATV.dial ARV @03:03:01.01 ARV.dial :carrier @03:03:01.37 AS.seek 0.5ml @03:03:26.90 AFV.select :carrierSolution @03:03:27.02 delay 2 seconds @03:03:29.05 AS.reconfigure ASconfig @03:03:29.11 AS.seek 1ml @03:03:58.15 delay 1 second @03:03:59.17 AFV.select :holdingCoil @03:03:59.27 delay 1 second @03:04:00.29 ATV.dial :waste @03:04:02.01 AS.reconfigure ASnorm @03:04:02.08 AS.seek :empty @03:04:52.82 AFV.select :carrierSolution @03:04:52.93 delay 2 seconds @03:04:54.96 AS.reconfigure ASconfig @03:04:55.02 AS.seek 0.5ml @03:05:24.12 delay 1 second @03:05:25.14 AFV.select :holdingCoil @03:05:25.25 Building Slug @03:05:25.26 ATV.dial :ARV @03:05:26.95 delay 2 seconds @03:05:28.97 ARV.dial :air1 @03:05:29.37 delay 2 seconds @03:05:31.44 AS.reconfigure ASslow2 @03:05:31.51 AS.seek 0.506ml @03:05:38.04 delay 2 seconds @03:05:40.06 ARV.dial :MM2 @03:05:41.57 delay 2 seconds @03:05:43.59 Adding MM2 @03:05:43.67 AS.seek 0.524ml @03:06:02.21 delay 25 seconds @03:06:27.23 ARV.dial :PP3 @03:06:27.63 Adding PP3 @03:06:27.64 delay 2 seconds @03:06:29.72 AS.seek 0.53ml @03:06:36.10 delay 10 seconds @03:06:46.13 ARV.dial :air2 @03:06:46.53 adding ARV air @03:06:46.54 delay 2 seconds @03:06:48.62 AS.seek 0.536ml @03:06:54.88 delay 5 seconds @03:06:59.90 ARV.dial :carrier @03:07:01.20 delay 7 seconds @03:07:08.28 AS.seek 0.594ml @03:08:06.98 delay 5 seconds @03:08:12.00 ATV.dial :ASV @03:08:12.42 delay 2 seconds @03:08:14.49 AS.seek 0.6ml @03:08:20.98 delay 4 seconds @03:08:25.00 ATV.dial ARV @03:08:25.56 delay 2 seconds @03:08:27.58 ARV.dial :carrier @03:08:27.59 delay 2 seconds @03:08:29.68 AS.seek 0.65ml @03:09:20.43 delay 4 seconds @03:09:24.45 ATV.dial PCR @03:09:25.09 ASV.dial :waste1 @03:09:25.10 ARV.dial :air1 @03:09:25.54 Positioning Slug by 0.324ml @03:09:25.63 AS.seek 0.326ml @03:14:52.37 delay 5 seconds @03:14:57.39 ATV.dial ARV @03:14:58.29 ASV.dial :PCRlock @03:14:58.68 delay 5 seconds @03:15:03.70 Running Mm Profile60C350 thermocycling @03:15:03.72 PCR.program Profile60C350 @03:15:03.87 Profile60C350 should complete in < 1:48:05 @03:15:03.89 PCR.run 2:20:06.25 @04:38:08.82 PCR.off @04:38:11.32 PCR for Mm completed -- closed /var/log/esp/Mm19may1503h.pcr @04:38:11.34 Full MFB Decontamination @04:38:11.36 Decon extraction column and decon AIV @04:38:11.39 SPE.hold 2:46:40,55C @04:38:11.45 ASV.dial :SPEdelivery @04:38:12.11 delay 20 seconds @04:38:32.14 AFV.select :carrierSolution @04:38:32.24 delay 2 seconds @04:38:34.27 AS.reconfigure ASconfig @04:38:34.32 AS.seek 0.5ml @04:38:44.62 delay 1 second @04:38:45.64 AFV.select :holdingCoil @04:38:45.74 ATV.dial :air1 @04:38:47.76 AS.reconfigure ASslow1 @04:38:47.82 AS.seek 0.52ml @04:38:52.11 ATV.dial :ASV @04:38:53.98 ASV.dial :SPErecovery @04:38:54.67 AS.seek 0.62ml @04:39:15.06 ATV.dial :waste @04:39:17.20 AS.reconfigure ASnorm @04:39:17.26 AS.seek :empty @04:39:48.92 AFV.select :holdingCoil @04:39:48.93 ATV.dial :waste @04:39:48.96 AS.reconfigure ASconfig @04:39:49.02 AS.seek 0ml @04:39:49.34 AFV.select :carrierSolution @04:39:49.45 delay 2 seconds @04:39:51.49 AS.seek 0.5ml @04:40:20.51 delay 1 second @04:40:21.53 AFV.select :holdingCoil @04:40:21.63 ATV.dial :air3 @04:40:22.39 AS.reconfigure ASslow1 @04:40:22.45 AS.seek 0.52ml @04:40:26.70 ATV.dial :air3 @04:40:26.79 AS.seek 0.53ml @04:40:29.09 delay 2 seconds @04:40:31.11 ATV.dial :bleach @04:40:31.60 AS.seek 0.71ml @04:41:08.08 delay 3 seconds @04:41:11.10 ATV.dial :air3 @04:41:11.72 delay 3 seconds @04:41:14.79 AS.seek 0.735ml @04:41:20.10 delay 3 seconds @04:41:23.12 ATV.dial :bleach @04:41:23.78 AS.seek 0.72ml @04:41:27.09 delay 3 seconds @04:41:30.11 ATV.dial ASV @04:41:31.25 ASV.dial :SPErecovery @04:41:31.34 AS.seek 0.365ml @04:42:43.01 ATV.dial :waste @04:42:44.84 AS.reconfigure ASnorm @04:42:44.91 AS.seek :empty @04:43:03.69 ATV.dial :air3 @04:43:04.40 AS.reconfigure ASslow1 @04:43:04.46 AS.seek 0.009ml @04:43:06.79 delay 2 seconds @04:43:08.81 ATV.dial :bleach @04:43:09.31 AS.seek 0.579ml @04:45:04.20 delay 3 seconds @04:45:07.22 ATV.dial :air3 @04:45:07.79 delay 3 seconds @04:45:10.87 AS.seek 0.604ml @04:45:16.19 delay 3 seconds @04:45:19.21 ATV.dial :bleach @04:45:19.84 AS.seek 0.589ml @04:45:23.15 delay 3 seconds @04:45:26.17 ATV.dial :ASV @04:45:27.23 ASV.dial :wasteR @04:45:27.63 AS.seek 0ml @04:47:26.40 delay 1:00 @04:48:26.43 ATV.dial ASV @04:48:26.44 AFV.select :carrierSolution @04:48:26.56 delay 2 seconds @04:48:28.59 AS.reconfigure ASconfig @04:48:28.64 AS.seek 1ml @04:49:26.42 delay 1 second @04:49:27.44 AFV.select :holdingCoil @04:49:27.54 delay 1 second @04:49:28.56 ASV.dial :wasteR @04:49:28.58 AS.reconfigure ASslow1 @04:49:28.65 AS.seek 0ml @04:52:50.56 delay 10 seconds @04:53:00.58 ATV.dial :air3 @04:53:02.14 AS.seek 0.01ml @04:53:04.47 delay 2 seconds @04:53:06.49 ATV.dial :bleach @04:53:07.15 AS.seek 0.58ml @04:55:02.08 delay 3 seconds @04:55:05.10 ATV.dial :air3 @04:55:05.67 delay 3 seconds @04:55:08.75 AS.seek 0.605ml @04:55:14.16 delay 3 seconds @04:55:17.18 ATV.dial :bleach @04:55:17.82 AS.seek 0.59ml @04:55:21.14 delay 3 seconds @04:55:24.16 ATV.dial ASV @04:55:25.23 ASV.dial :SPEdelivery @04:55:25.71 AS.seek 0ml @04:57:24.59 delay 1:00 @04:58:24.62 AFV.select :carrierSolution @04:58:24.72 delay 2 seconds @04:58:26.75 AS.reconfigure ASconfig @04:58:26.81 AS.seek 1ml @04:59:24.58 delay 1 second @04:59:25.60 AFV.select :holdingCoil @04:59:25.70 delay 1 second @04:59:26.72 ATV.dial ASV @04:59:26.73 ASV.dial :SPEdelivery @04:59:26.76 AS.reconfigure ASslow1 @04:59:26.83 AS.seek 0ml @05:02:48.25 delay 10 seconds @05:02:58.28 ATV.dial :air2 @05:02:59.15 AS.reconfigure ASslow0 @05:02:59.21 AS.seek 0.8ml @05:04:19.92 delay 3 seconds @05:04:22.94 ATV.dial :ASV @05:04:23.95 ASV.dial :SPEdelivery @05:04:23.97 AS.reconfigure ASslow1 @05:04:24.04 AS.seek 0.03ml @05:06:59.20 delay 20 seconds @05:07:19.23 ATV.dial :waste,via: :air3 @05:07:21.03 AS.reconfigure ASconfig @05:07:21.09 AS.seek :empty @05:07:23.17 SPE.coast @05:07:23.26 Removing Bleach from Mixing Coil @05:07:23.27 AFV.select :carrierSolution @05:07:23.38 delay 2 seconds @05:07:25.41 AS.seek 0.5ml @05:07:54.51 delay 1 second @05:07:55.53 AFV.select :holdingCoil @05:07:55.63 ATV.dial :ASV @05:07:57.37 ASV.dial :SPErecovery @05:07:58.01 AS.reconfigure ASslow1 @05:07:58.07 AS.seek 0.73ml @05:08:44.61 ASV.dial :wasteD @05:08:45.19 AS.reconfigure ASnorm @05:08:45.26 AS.seek 0ml @05:09:22.37 AFV.select :carrierSolution @05:09:22.48 delay 2 seconds @05:09:24.51 AS.reconfigure ASconfig @05:09:24.56 AS.seek 0.5ml @05:09:53.61 delay 1 second @05:09:54.63 AFV.select :holdingCoil @05:09:54.73 ATV.dial ASV @05:09:54.75 ASV.dial :SPErecovery @05:09:55.40 AS.reconfigure ASslow0 @05:09:55.46 AS.seek 0.3ml @05:10:15.90 ATV.dial :air2 @05:10:16.96 AS.reconfigure ASslow1 @05:10:17.02 AS.seek 0.32ml @05:10:21.40 ATV.dial ASV @05:10:22.45 AS.reconfigure ASslow0 @05:10:22.51 AS.seek 0.57ml @05:10:48.00 ASV.dial :wasteD @05:10:48.59 AS.reconfigure ASnorm @05:10:48.65 AS.seek 0ml @05:11:17.69 AFV.select :carrierSolution @05:11:17.80 delay 2 seconds @05:11:19.83 AS.reconfigure ASconfig @05:11:19.88 AS.seek 0.5ml @05:11:48.97 delay 1 second @05:11:49.99 AFV.select :holdingCoil @05:11:50.09 ATV.dial ASV @05:11:50.11 ASV.dial :SPErecovery @05:11:50.76 AS.reconfigure ASslow0 @05:11:50.82 AS.seek 0.3ml @05:12:11.23 ATV.dial :air2 @05:12:12.29 AS.reconfigure ASslow1 @05:12:12.35 AS.seek 0.32ml @05:12:16.56 ATV.dial ASV @05:12:18.13 AS.reconfigure ASslow0 @05:12:18.21 AS.seek 0.57ml @05:12:43.70 ASV.dial :wasteD @05:12:44.38 AS.reconfigure ASnorm @05:12:44.44 AS.seek 0ml @05:13:13.53 ATV.dial :air2 @05:13:14.54 AS.reconfigure ASslow0 @05:13:14.60 AS.seek 0.3ml @05:13:45.14 ATV.dial :ASV @05:13:46.14 ASV.dial :wasteD @05:13:46.16 AS.reconfigure ASconfig @05:13:46.23 AS.seek 0.05ml @05:14:00.92 ATV.dial :waste @05:14:02.84 AS.seek 0ml @05:14:06.02 ASV.dial :SPEdelivery @05:14:07.01 deconLysateINT @05:14:07.02 ATV.dial :air3 @05:14:07.73 AS.reconfigure ASslow1 @05:14:07.79 AS.seek 0.01ml @05:14:10.12 delay 2 seconds @05:14:12.14 ATV.dial :bleach @05:14:12.61 AS.seek 0.96ml @05:17:23.92 delay 3 seconds @05:17:26.94 ATV.dial :air3 @05:17:27.50 delay 3 seconds @05:17:30.57 AS.seek 0.985ml @05:17:35.87 delay 3 seconds @05:17:38.89 ATV.dial :bleach @05:17:39.51 AS.seek 0.97ml @05:17:42.83 delay 3 seconds @05:17:45.85 ATV.dial AIV @05:17:47.12 AIV.dial :coilToMFB @05:17:47.13 AWV.select :waste @05:17:47.15 AFV.select :carrierSolution @05:17:47.28 delay 2 seconds @05:17:49.31 AS.reconfigure ASconfig @05:17:49.37 AS.seek 1ml @05:17:51.40 delay 1 second @05:17:52.42 AFV.select :holdingCoil @05:17:52.52 delay 1 second @05:17:53.55 AS.reconfigure ASslow0 @05:17:53.60 AS.seek :empty @05:19:34.59 AFV.select :carrierSolution @05:19:34.71 delay 2 seconds @05:19:36.74 AS.reconfigure ASconfig @05:19:36.80 AS.seek 1ml @05:20:34.67 delay 1 second @05:20:35.69 AFV.select :holdingCoil @05:20:35.79 delay 1 second @05:20:36.82 AS.reconfigure ASslow0 @05:20:36.87 AS.seek :empty @05:22:17.84 AFV.select :carrierSolution @05:22:17.95 delay 2 seconds @05:22:19.98 AS.reconfigure ASconfig @05:22:20.04 AS.seek 1ml @05:23:17.87 delay 1 second @05:23:18.89 AFV.select :holdingCoil @05:23:18.99 delay 1 second @05:23:20.02 AS.reconfigure ASslow0 @05:23:20.07 AS.seek :empty @05:25:01.06 ATV.dial :air2 @05:25:01.75 AS.reconfigure ASslow1 @05:25:01.81 AS.seek 0.009ml @05:25:04.12 AS.reconfigure ASslow0 @05:25:04.18 AS.seek 1ml @05:26:44.14 ATV.dial AIV @05:26:44.93 AS.seek 0.02ml @05:28:23.84 ATV.dial :waste @05:28:25.54 AS.seek 0ml @05:28:27.87 AWV.select :air @05:28:28.03 Cleaning PCR @05:28:28.04 AFV.select :carrierSolution @05:28:28.15 delay 2 seconds @05:28:30.18 AS.reconfigure ASconfig @05:28:30.23 AS.seek 0.5ml @05:28:59.28 delay 1 second @05:29:00.30 AFV.select :holdingCoil @05:29:00.40 ASV.dial :waste1 @05:29:00.86 ATV.dial :air3 @05:29:01.56 AS.reconfigure ASslow1 @05:29:01.62 AS.seek 0.51ml @05:29:03.98 delay 2 seconds @05:29:06.00 ATV.dial :bleach @05:29:06.47 AS.seek 0.81ml @05:30:07.09 delay 3 seconds @05:30:10.11 ATV.dial :air3 @05:30:10.66 delay 3 seconds @05:30:13.73 AS.seek 0.835ml @05:30:19.07 delay 3 seconds @05:30:22.09 ATV.dial :bleach @05:30:23.24 AS.seek 0.82ml @05:30:26.55 delay 3 seconds @05:30:29.57 ARV.dial :waste @05:30:30.19 delay 3 seconds @05:30:33.21 ATV.dial :ARV @05:30:34.11 AS.reconfigure ASconfig @05:30:34.17 AS.seek 0.47ml @05:30:54.61 AFV.select :carrierSolution @05:30:54.72 delay 2 seconds @05:30:56.75 AS.seek 1ml @05:31:27.55 delay 1 second @05:31:28.57 AFV.select :holdingCoil @05:31:28.67 delay 1 second @05:31:29.70 AS.reconfigure ASfast @05:31:29.75 AS.seek 0ml @05:31:57.11 AFV.select :carrierSolution @05:31:57.23 delay 2 seconds @05:31:59.26 AS.reconfigure ASconfig @05:31:59.31 AS.seek 1ml @05:32:57.08 delay 1 second @05:32:58.10 AFV.select :holdingCoil @05:32:58.20 delay 1 second @05:32:59.23 AS.reconfigure ASfast @05:32:59.28 AS.seek 0ml @05:33:26.64 AFV.select :carrierSolution @05:33:26.75 delay 2 seconds @05:33:28.78 AS.reconfigure ASconfig @05:33:28.83 AS.seek 1ml @05:34:26.61 delay 1 second @05:34:27.63 AFV.select :holdingCoil @05:34:27.73 delay 1 second @05:34:28.76 AS.reconfigure ASfast @05:34:28.81 AS.seek 0ml @05:34:56.17 ARV.dial between carrier and air1 @05:34:56.67 ATV.dial :air3 @05:34:57.98 AS.reconfigure ASslow1 @05:34:58.04 AS.seek 0.01ml @05:35:00.41 delay 2 seconds @05:35:02.43 ATV.dial :bleach @05:35:03.06 AS.seek 0.81ml @05:37:44.23 delay 3 seconds @05:37:47.25 ATV.dial :air3 @05:37:47.81 delay 3 seconds @05:37:50.88 AS.seek 0.835ml @05:37:56.20 delay 3 seconds @05:37:59.22 ATV.dial :bleach @05:37:59.84 AS.seek 0.82ml @05:38:03.16 delay 3 seconds @05:38:06.18 ATV.dial PCR @05:38:06.61 AS.reconfigure ASconfig @05:38:06.67 AS.seek :empty @05:38:54.16 PCR.program :bleachProfile @05:38:54.22 PCR program should complete in < 2:45 @05:38:54.23 PCR.run 10:00 @05:41:56.04 PCR.off @05:41:56.07 AFV.select :carrierSolution @05:41:56.19 delay 2 seconds @05:41:58.22 AS.seek 1ml @05:42:56.06 delay 1 second @05:42:57.08 AFV.select :holdingCoil @05:42:57.18 delay 1 second @05:42:58.21 AS.reconfigure ASfast @05:42:58.26 AS.seek 0ml @05:43:25.61 delay 3 seconds @05:43:28.63 AFV.select :carrierSolution @05:43:28.73 delay 2 seconds @05:43:30.76 AS.reconfigure ASconfig @05:43:30.82 AS.seek 1ml @05:44:28.61 delay 1 second @05:44:29.63 AFV.select :holdingCoil @05:44:29.73 delay 1 second @05:44:30.76 AS.reconfigure ASfast @05:44:30.81 AS.seek 0ml @05:44:58.16 delay 3 seconds @05:45:01.18 AFV.select :carrierSolution @05:45:01.28 delay 2 seconds @05:45:03.31 AS.reconfigure ASconfig @05:45:03.37 AS.seek 1ml @05:46:01.12 delay 1 second @05:46:02.14 AFV.select :holdingCoil @05:46:02.24 delay 1 second @05:46:03.27 AS.reconfigure ASfast @05:46:03.32 AS.seek 0ml @05:46:30.69 delay 3 seconds @05:46:33.71 AFV.select :carrierSolution @05:46:33.81 delay 2 seconds @05:46:35.84 AS.reconfigure ASconfig @05:46:35.90 AS.seek 1ml @05:47:33.70 delay 1 second @05:47:34.72 AFV.select :holdingCoil @05:47:34.82 delay 1 second @05:47:35.85 AS.reconfigure ASfast @05:47:35.90 AS.seek 0ml @05:48:03.27 delay 3 seconds @05:48:06.29 AFV.select :carrierSolution @05:48:06.39 delay 2 seconds @05:48:08.42 AS.reconfigure ASconfig @05:48:08.48 AS.seek 1ml @05:49:06.80 delay 1 second @05:49:07.82 AFV.select :holdingCoil @05:49:07.92 delay 1 second @05:49:08.95 AS.reconfigure ASnorm @05:49:09.00 AS.seek 0ml @05:49:59.73 AFV.select :carrierSolution @05:49:59.84 delay 2 seconds @05:50:01.87 AS.reconfigure ASconfig @05:50:01.92 AS.seek 1ml @05:50:59.72 delay 1 second @05:51:00.74 AFV.select :holdingCoil @05:51:00.84 delay 1 second @05:51:01.86 ATV.dial :waste @05:51:03.13 AS.reconfigure ASnorm @05:51:03.19 AS.seek 0.5ml @05:51:28.77 ATV.dial :waste2 @05:51:30.10 AS.seek 0ml @05:51:55.64
Asleep after completing 18tun assays @05:51:55.65 GoodNews.email "{1 => 2, 2 => 1}\nCan@05:43:30, 20.9C, 28% humidity, 14.8psia, 12.070V, 0.411A, 0.411A avg, 4.97W",Subject: "Asleep after completing 18tun assays" @05:51:55.69
Mission runtime was 11:38:25.186669 @05:51:55.73 SocketError in Qmail -- getaddrinfo: Temporary failure in name resolution @05:51:55.76 Retry #1 of 10 after 10:00 @05:52:15.77
Sending Email @05:55:15.79 Email::Error in MAIN -- flush failed! @05:55:15.84 Session Ended @16:55:58.40CEST15-May-19
ESP2 v3.30 I2C Gateway v3.06 for core bus @16:56:00.17 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @16:56:00.43 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @16:56:05.62 Polling Can every 10:00 @16:56:07.38 SPE.coast @16:56:07.43 SPE.configure SPEconfig @16:56:07.51 AS.reconfigure ASconfig @16:56:07.55 AS.hold @16:56:07.59 AS.configure ASconfig @16:56:07.67 SS.reconfigure SSconfig @16:56:07.71 SS.hold @16:56:07.76 SS.configure SSconfig @16:56:07.84 CC.reconfigure CCconfig @16:56:07.88 CC.hold @16:56:07.94 CC.configure CCconfig @16:56:08.02 CS.reconfigure CSconfig @16:56:08.06 CS.hold @16:56:08.13 CS.configure CSconfig @16:56:08.55 PC.reconfigure PCconfig @16:56:08.59 PC.hold @16:56:08.66 PC.configure PCconfig @16:56:08.74 PS.configure PSconfig @16:56:08.84 Forearm.reconfigure LooseForearm @16:56:08.88 Forearm.hold @16:56:08.96 Forearm.configure LooseForearm @16:56:09.12 Elbow.reconfigure LooseElbow @16:56:09.19 Elbow.hold @16:56:09.28 Elbow.configure LooseElbow @16:56:09.47 SC.reconfigure SCloose @16:56:09.55 SC.hold @16:56:09.64 SC.configure SCloose @16:56:09.79 SE.reconfigure SEconfig @16:56:09.83 SE.hold @16:56:09.89 SE.configure SEconfig @16:56:09.93 CH.coast @16:56:09.97 CH.configure CHconfig @16:56:10.00 PH.coast @16:56:10.05 PH.configure PHconfig @16:56:10.09 PCR.off @16:56:10.11 PCR.pidtemp= 50,0.1,0,300,25,25 @16:56:10.14 PCR.caltemp= 2.8814,0.0372,0 @16:56:10.17 All dwarf controllers running firmware version 3.75 @16:56:10.29 Cleanly exited at 16:49:43CEST15-May-19 @16:56:10.32 Loading /home/esp/esp2/mission/190515totalsampletest.rb mission @16:56:10.37 Preparing to sleep until +0:00:15 @16:56:10.39 GoodNews.email "Starting 190515totalsampletest w/pucks in tube 2\n{}\nCan@16:56:05, 21.8C, 26% humidity, 14.8psia, 12.041V, 0.487A, 0.487A avg, 5.86W",Subject: "190515totalsampletest begun" @16:56:10.47 AS.coast @16:56:10.49
Asleep til +0:00:15 @16:56:10.52 Continuing at 16:56 @16:56:10.54 SS.coast @16:56:10.61 CC.coast @16:56:10.68 CS.coast @16:56:10.71 PC.coast @16:56:10.74 Forearm.coast @16:56:10.77 Elbow.coast @16:56:10.80 SC.coast @16:56:10.83 SE.coast @16:56:11.23 Gate.power :core,:OFF @16:56:11.25 Gate.power :raw,:OFF @16:56:11.26 Shutdown @16:56:12.93 Session Begun @16:56:17.07 -> showlog 100 @16:56:17.17 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/190515totalsampletest.rb:112\n{}\nCan@16:56:10, 21.8C, 26% humidity, 14.8psia, 12.041V, 0.481A, 0.481A avg, 5.79W",Subject: "Asleep until 16:56" @16:56:25.36
Powering core bus @16:56:30.20 Gate.power :raw,:ON @16:56:30.84 SPE.coast @16:56:30.91 SPE.configure SPEconfig @16:56:31.01 AS.configure ASconfig @16:56:31.12 SS.configure SSconfig @16:56:31.23 CC.configure CCconfig @16:56:31.33 CS.configure CSconfig @16:56:31.43 PC.configure PCconfig @16:56:31.53 PS.configure PSconfig @16:56:31.65 Forearm.configure LooseForearm @16:56:31.75 Elbow.configure LooseElbow @16:56:31.90 SC.configure SCloose @16:56:32.00 SE.configure SEconfig @16:56:32.05 CH.coast @16:56:32.11 CH.configure CHconfig @16:56:32.15 PH.coast @16:56:32.23 PH.configure PHconfig @16:56:32.28 PCR.off @16:56:32.32 PCR.pidtemp= 50,0.1,0,300,25,25 @16:56:32.35 PCR.caltemp= 2.8814,0.0372,0 @16:56:32.39 All dwarf controllers running firmware version 3.75 @16:56:32.52 Valve.reset @16:56:32.69 PCR.off @16:56:32.72 PCR.pidtemp= 50,0.1,0,300,25,25 @16:56:32.76 PCR.caltemp= 2.8814,0.0372,0 @16:56:32.92 Intake.select :close @16:56:32.98 Exhaust.select :close @16:56:33.01 CTV.dial :air @16:56:33.10 PSV.dial :air @16:56:33.22 CSV.dial :waste1 @16:56:33.25
Forearm.reconfigure LooseForearm @16:56:33.36 Forearm.home.seek :retract @16:56:33.43 SSV.dial :spit,avoiding: :exhaust @16:56:33.89 PS.reconfigure PSconfig @16:56:34.00 PS.home.seek 0ml @16:56:34.12 Hand.close @16:56:34.17 CS.reconfigure CSfast @16:56:34.20
Elbow.jog 1500 @16:56:34.23 SS.reconfigure SSconfig @16:56:34.40 SS.home.seek 0ml @16:56:34.42 CS.home.seek 0ml @16:56:34.90
Elbow.reconfigure LooseElbow @16:56:34.96 Elbow.home.seek :clear @16:56:38.40 SE.reconfigure SEconfig @16:56:38.46 SE.home.seek :down @16:56:39.16 SC.reconfigure SCsloppy @16:56:39.22 SC.home.seek :initial @16:56:43.38 PS.coast @16:56:49.54
Forearm.seek :retract @16:56:49.63 Elbow.seek :hammer @16:56:50.93 Forearm.reconfigure TightForearm @16:56:50.99 Forearm.seek 5 + 350 counts @16:56:52.20 Forearm.seek :retract @16:56:53.34 CC.reconfigure CCconfig @16:56:53.36 PC.reconfigure PCconfig @16:56:53.47
CC.home.seek :open @16:56:53.49 PC.home.seek :open @16:56:56.77
Phase [mbaPhase] @16:56:56.79 GoodNews.email "At 16:56:56 began in tube 2: mbaPhase\n/home/esp/esp2/mission/190515totalsampletest.rb:112\n{}\nCan@16:56:56, 21.8C, 26% humidity, 14.8psia, 11.947V, 0.827A, 0.827A avg, 9.89W",Subject: "Started 190515totalsampletest phase mbaPhase" @16:56:56.83CEST15-May-19
==================> STARTED MBAsuperphase (phase #1) <=== @16:56:57.44 Readying the MFB @16:56:57.45 Homing MFB Syringe @16:56:57.46 AFV.select :holdingCoil @16:56:57.64 ATV.dial :air2 @16:56:58.93 AS.reconfigure ASconfig @16:56:58.98 AS.home.seek :home @16:57:04.56 AFV.select :carrierSolution @16:57:04.68 delay 2 seconds @16:57:06.71 AS.seek 1ml @16:57:59.58 delay 1 second @16:58:00.60 AFV.select :holdingCoil @16:58:00.71 delay 1 second @16:58:01.72 ATV.dial :waste @16:58:02.98 AS.reconfigure ASnorm @16:58:03.04 AS.home.seek :empty @16:58:55.64 ATV.dial :waste @16:58:55.65 ARV.dial between carrier and air1 @16:58:55.76 ASV.dial :waste1 @16:58:55.84 Full MFB Decontamination @16:58:55.85 Decon extraction column and decon AIV @16:58:55.89 SPE.hold 2:46:40,55C @16:58:55.96 ASV.dial :SPEdelivery @16:58:56.37 delay 20 seconds @16:59:16.40 AFV.select :carrierSolution @16:59:16.51 delay 2 seconds @16:59:18.53 AS.reconfigure ASconfig @16:59:18.59 AS.seek 0.5ml @16:59:47.67 delay 1 second @16:59:48.69 AFV.select :holdingCoil @16:59:48.79 ATV.dial :air1 @16:59:49.46 AS.reconfigure ASslow1 @16:59:49.52 AS.seek 0.52ml @16:59:53.84 ATV.dial :ASV @16:59:55.61 ASV.dial :SPErecovery @16:59:56.31 AS.seek 0.62ml @17:00:16.78 ATV.dial :waste @17:00:18.95 AS.reconfigure ASnorm @17:00:19.01 AS.seek :empty @17:00:50.67 AFV.select :holdingCoil @17:00:50.68 ATV.dial :waste @17:00:50.71 AS.reconfigure ASconfig @17:00:50.78 AS.seek 0ml @17:00:51.09 AFV.select :carrierSolution @17:00:51.22 delay 2 seconds @17:00:53.24 AS.seek 0.5ml @17:01:22.28 delay 1 second @17:01:23.30 AFV.select :holdingCoil @17:01:23.40 ATV.dial :air3 @17:01:24.17 AS.reconfigure ASslow1 @17:01:24.23 AS.seek 0.52ml @17:01:28.54 ATV.dial :air3 @17:01:28.63 AS.seek 0.53ml @17:01:30.83 delay 2 seconds @17:01:32.85 ATV.dial :bleach @17:01:33.35 AS.seek 0.71ml @17:02:09.94 delay 3 seconds @17:02:12.96 ATV.dial :air3 @17:02:13.58 delay 3 seconds @17:02:16.65 AS.seek 0.735ml @17:02:21.98 delay 3 seconds @17:02:25.00 ATV.dial :bleach @17:02:25.67 AS.seek 0.72ml @17:02:28.98 delay 3 seconds @17:02:32.00 ATV.dial ASV @17:02:33.15 ASV.dial :SPErecovery @17:02:33.24 AS.seek 0.365ml @17:03:22.32 -> exit @17:03:22.34 Session Ended @17:03:44.86
ATV.dial :waste @17:03:46.69 AS.reconfigure ASnorm @17:03:46.74 AS.seek :empty @17:04:05.50 ATV.dial :air3 @17:04:06.20 AS.reconfigure ASslow1 @17:04:06.24 AS.seek 0.009ml @17:04:08.59 delay 2 seconds @17:04:10.61 ATV.dial :bleach @17:04:11.07 AS.seek 0.579ml @17:06:05.95 delay 3 seconds @17:06:08.97 ATV.dial :air3 @17:06:09.53 delay 3 seconds @17:06:12.60 AS.seek 0.604ml @17:06:17.90 delay 3 seconds @17:06:20.92 ATV.dial :bleach @17:06:21.54 AS.seek 0.589ml @17:06:24.86 delay 3 seconds @17:06:27.88 ATV.dial :ASV @17:06:28.95 ASV.dial :wasteR @17:06:29.43 AS.seek 0ml @17:08:28.20 delay 1:00 @17:09:28.61 ATV.dial ASV @17:09:28.62 AFV.select :carrierSolution @17:09:28.73 delay 2 seconds @17:09:30.75 AS.reconfigure ASconfig @17:09:30.80 AS.seek 1ml @17:10:28.56 delay 1 second @17:10:29.58 AFV.select :holdingCoil @17:10:29.69 delay 1 second @17:10:30.70 ASV.dial :wasteR @17:10:30.72 AS.reconfigure ASslow1 @17:10:30.77 AS.seek 0ml @17:13:52.12 delay 10 seconds @17:14:02.15 ATV.dial :air3 @17:14:03.70 AS.seek 0.01ml @17:14:06.01 delay 2 seconds @17:14:08.03 ATV.dial :bleach @17:14:08.67 AS.seek 0.58ml @17:16:03.59 delay 3 seconds @17:16:06.61 ATV.dial :air3 @17:16:07.17 delay 3 seconds @17:16:10.25 AS.seek 0.605ml @17:16:15.54 delay 3 seconds @17:16:18.56 ATV.dial :bleach @17:16:19.18 AS.seek 0.59ml @17:16:22.50 delay 3 seconds @17:16:25.52 ATV.dial ASV @17:16:26.61 ASV.dial :SPEdelivery @17:16:26.99 AS.seek 0ml @17:18:25.92 delay 1:00 @17:19:25.94 AFV.select :carrierSolution @17:19:26.05 delay 2 seconds @17:19:28.07 AS.reconfigure ASconfig @17:19:28.12 AS.seek 1ml @17:20:25.87 delay 1 second @17:20:26.89 AFV.select :holdingCoil @17:20:27.00 delay 1 second @17:20:28.01 ATV.dial ASV @17:20:28.02 ASV.dial :SPEdelivery @17:20:28.04 AS.reconfigure ASslow1 @17:20:28.09 AS.seek 0ml @17:23:49.47 delay 10 seconds @17:23:59.49 ATV.dial :air2 @17:24:00.35 AS.reconfigure ASslow0 @17:24:00.39 AS.seek 0.8ml @17:25:21.17 delay 3 seconds @17:25:24.19 ATV.dial :ASV @17:25:25.20 ASV.dial :SPEdelivery @17:25:25.22 AS.reconfigure ASslow1 @17:25:25.26 AS.seek 0.03ml @17:28:00.40 delay 20 seconds @17:28:20.42 ATV.dial :waste,via: :air3 @17:28:22.22 AS.reconfigure ASconfig @17:28:22.27 AS.seek :empty @17:28:24.34 SPE.coast @17:28:24.41 Removing Bleach from Mixing Coil @17:28:24.42 AFV.select :carrierSolution @17:28:24.52 delay 2 seconds @17:28:26.54 AS.seek 0.5ml @17:28:55.65 delay 1 second @17:28:56.67 AFV.select :holdingCoil @17:28:56.77 ATV.dial :ASV @17:28:58.54 ASV.dial :SPErecovery @17:28:59.20 AS.reconfigure ASslow1 @17:28:59.24 AS.seek 0.73ml @17:29:45.78 ASV.dial :wasteD @17:29:46.34 AS.reconfigure ASnorm @17:29:46.39 AS.seek 0ml @17:30:23.50 AFV.select :carrierSolution @17:30:23.61 delay 2 seconds @17:30:25.63 AS.reconfigure ASconfig @17:30:25.68 AS.seek 0.5ml @17:30:54.73 delay 1 second @17:30:55.75 AFV.select :holdingCoil @17:30:55.85 ATV.dial ASV @17:30:55.86 ASV.dial :SPErecovery @17:30:56.49 AS.reconfigure ASslow0 @17:30:56.53 AS.seek 0.3ml @17:31:16.93 ATV.dial :air2 @17:31:17.99 AS.reconfigure ASslow1 @17:31:18.03 AS.seek 0.32ml @17:31:22.32 ATV.dial ASV @17:31:23.38 AS.reconfigure ASslow0 @17:31:23.42 AS.seek 0.57ml @17:31:48.87 ASV.dial :wasteD @17:31:49.46 AS.reconfigure ASnorm @17:31:49.50 AS.seek 0ml @17:32:18.54 AFV.select :carrierSolution @17:32:18.65 delay 2 seconds @17:32:20.67 AS.reconfigure ASconfig @17:32:20.72 AS.seek 0.5ml @17:32:49.73 delay 1 second @17:32:50.75 AFV.select :holdingCoil @17:32:50.85 ATV.dial ASV @17:32:50.86 ASV.dial :SPErecovery @17:32:51.49 AS.reconfigure ASslow0 @17:32:51.53 AS.seek 0.3ml @17:33:11.95 ATV.dial :air2 @17:33:12.99 AS.reconfigure ASslow1 @17:33:13.03 AS.seek 0.32ml @17:33:17.32 ATV.dial ASV @17:33:18.38 AS.reconfigure ASslow0 @17:33:18.42 AS.seek 0.57ml @17:33:43.84 ASV.dial :wasteD @17:33:44.43 AS.reconfigure ASnorm @17:33:44.47 AS.seek 0ml @17:34:13.51 ATV.dial :air2 @17:34:14.52 AS.reconfigure ASslow0 @17:34:14.56 AS.seek 0.3ml @17:34:45.11 ATV.dial :ASV @17:34:46.11 ASV.dial :wasteD @17:34:46.13 AS.reconfigure ASconfig @17:34:46.17 AS.seek 0.05ml @17:35:00.86 ATV.dial :waste @17:35:02.77 AS.seek 0ml @17:35:05.91 ASV.dial :SPEdelivery @17:35:06.90 deconLysateINT @17:35:06.91 ATV.dial :air3 @17:35:07.59 AS.reconfigure ASslow1 @17:35:07.63 AS.seek 0.01ml @17:35:09.95 delay 2 seconds @17:35:11.97 ATV.dial :bleach @17:35:12.43 AS.seek 0.96ml @17:38:23.72 delay 3 seconds @17:38:26.74 ATV.dial :air3 @17:38:27.72 delay 3 seconds @17:38:30.80 AS.seek 0.985ml @17:38:36.08 delay 3 seconds @17:38:39.10 ATV.dial :bleach @17:38:39.73 AS.seek 0.97ml @17:38:43.12 delay 3 seconds @17:38:46.14 ATV.dial AIV @17:38:47.42 AIV.dial :coilToMFB @17:38:47.61 AWV.select :waste @17:38:47.71 AFV.select :carrierSolution @17:38:47.82 delay 2 seconds @17:38:49.84 AS.reconfigure ASconfig @17:38:49.89 AS.seek 1ml @17:38:51.90 delay 1 second @17:38:52.91 AFV.select :holdingCoil @17:38:53.02 delay 1 second @17:38:54.04 AS.reconfigure ASslow0 @17:38:54.08 AS.seek :empty @17:40:35.07 AFV.select :carrierSolution @17:40:35.18 delay 2 seconds @17:40:37.20 AS.reconfigure ASconfig @17:40:37.25 AS.seek 1ml @17:41:35.04 delay 1 second @17:41:36.06 AFV.select :holdingCoil @17:41:36.17 delay 1 second @17:41:37.19 AS.reconfigure ASslow0 @17:41:37.23 AS.seek :empty @17:43:18.18 AFV.select :carrierSolution @17:43:18.29 delay 2 seconds @17:43:20.31 AS.reconfigure ASconfig @17:43:20.36 AS.seek 1ml @17:44:18.18 delay 1 second @17:44:19.20 AFV.select :holdingCoil @17:44:19.31 delay 1 second @17:44:20.33 AS.reconfigure ASslow0 @17:44:20.37 AS.seek :empty @17:46:01.32 ATV.dial :air2 @17:46:02.01 AS.reconfigure ASslow1 @17:46:02.05 AS.seek 0.009ml @17:46:04.34 AS.reconfigure ASslow0 @17:46:04.39 AS.seek 1ml @17:47:44.32 ATV.dial AIV @17:47:45.12 AS.seek 0.02ml @17:49:24.00 ATV.dial :waste @17:49:25.69 AS.seek 0ml @17:49:27.98 AWV.select :air @17:49:28.13 Cleaning PCR @17:49:28.14 AFV.select :carrierSolution @17:49:28.24 delay 2 seconds @17:49:30.26 AS.reconfigure ASconfig @17:49:30.31 AS.seek 0.5ml @17:49:59.31 delay 1 second @17:50:00.33 AFV.select :holdingCoil @17:50:00.43 ASV.dial :waste1 @17:50:00.79 ATV.dial :air3 @17:50:01.48 AS.reconfigure ASslow1 @17:50:01.52 AS.seek 0.51ml @17:50:03.87 delay 2 seconds @17:50:05.89 ATV.dial :bleach @17:50:06.35 AS.seek 0.81ml @17:51:06.95 delay 3 seconds @17:51:09.97 ATV.dial :air3 @17:51:10.53 delay 3 seconds @17:51:13.60 AS.seek 0.835ml @17:51:18.90 delay 3 seconds @17:51:21.92 ATV.dial :bleach @17:51:22.53 AS.seek 0.82ml @17:51:25.84 delay 3 seconds @17:51:28.86 ARV.dial :waste @17:51:29.36 delay 3 seconds @17:51:32.38 ATV.dial :ARV @17:51:33.29 AS.reconfigure ASconfig @17:51:33.33 AS.seek 0.47ml @17:51:53.72 AFV.select :carrierSolution @17:51:53.83 delay 2 seconds @17:51:55.85 AS.seek 1ml @17:52:26.62 delay 1 second @17:52:27.64 AFV.select :holdingCoil @17:52:27.75 delay 1 second @17:52:28.77 AS.reconfigure ASfast @17:52:28.82 AS.seek 0ml @17:52:56.14 AFV.select :carrierSolution @17:52:56.25 delay 2 seconds @17:52:58.27 AS.reconfigure ASconfig @17:52:58.32 AS.seek 1ml @17:53:56.06 delay 1 second @17:53:57.08 AFV.select :holdingCoil @17:53:57.19 delay 1 second @17:53:58.21 AS.reconfigure ASfast @17:53:58.26 AS.seek 0ml @17:54:25.62 AFV.select :carrierSolution @17:54:25.73 delay 2 seconds @17:54:27.75 AS.reconfigure ASconfig @17:54:27.80 AS.seek 1ml @17:55:25.57 delay 1 second @17:55:26.59 AFV.select :holdingCoil @17:55:26.70 delay 1 second @17:55:27.72 AS.reconfigure ASfast @17:55:27.77 AS.seek 0ml @17:55:55.11 ARV.dial between carrier and air1 @17:55:55.61 ATV.dial :air3 @17:55:56.92 AS.reconfigure ASslow1 @17:55:56.96 AS.seek 0.01ml @17:55:59.34 delay 2 seconds @17:56:01.36 ATV.dial :bleach @17:56:01.98 AS.seek 0.81ml @17:58:43.12 delay 3 seconds @17:58:46.14 ATV.dial :air3 @17:58:46.70 delay 3 seconds @17:58:49.77 AS.seek 0.835ml @17:58:55.18 delay 3 seconds @17:58:58.20 ATV.dial :bleach @17:58:58.82 AS.seek 0.82ml @17:59:02.12 delay 3 seconds @17:59:05.14 ATV.dial PCR @17:59:05.54 AS.reconfigure ASconfig @17:59:05.58 AS.seek :empty @17:59:53.03 PCR.program :bleachProfile @17:59:53.08 PCR program should complete in < 2:45 PCR.run 10:00 @18:02:55.30 PCR.off @18:02:55.33 AFV.select :carrierSolution @18:02:55.44 delay 2 seconds @18:02:57.46 AS.seek 1ml @18:03:55.29 delay 1 second @18:03:56.31 AFV.select :holdingCoil @18:03:56.42 delay 1 second @18:03:57.44 AS.reconfigure ASfast @18:03:57.49 AS.seek 0ml @18:04:24.81 delay 3 seconds @18:04:27.83 AFV.select :carrierSolution @18:04:27.94 delay 2 seconds @18:04:29.96 AS.reconfigure ASconfig @18:04:30.01 AS.seek 1ml @18:05:27.79 delay 1 second @18:05:28.81 AFV.select :holdingCoil @18:05:28.92 delay 1 second @18:05:29.94 AS.reconfigure ASfast @18:05:29.99 AS.seek 0ml @18:05:57.48 delay 3 seconds @18:06:00.50 AFV.select :carrierSolution @18:06:00.61 delay 2 seconds @18:06:02.63 AS.reconfigure ASconfig @18:06:02.69 AS.seek 1ml @18:07:00.43 delay 1 second @18:07:01.45 AFV.select :holdingCoil @18:07:01.56 delay 1 second @18:07:02.58 AS.reconfigure ASfast @18:07:02.63 AS.seek 0ml @18:07:29.98 delay 3 seconds @18:07:33.00 AFV.select :carrierSolution @18:07:33.11 delay 2 seconds @18:07:35.13 AS.reconfigure ASconfig @18:07:35.18 AS.seek 1ml @18:08:32.95 delay 1 second @18:08:33.97 AFV.select :holdingCoil @18:08:34.08 delay 1 second @18:08:35.10 AS.reconfigure ASfast @18:08:35.15 AS.seek 0ml @18:09:02.50 delay 3 seconds @18:09:05.52 AFV.select :carrierSolution @18:09:05.63 delay 2 seconds @18:09:07.65 AS.reconfigure ASconfig @18:09:07.71 AS.seek 1ml @18:10:05.48 delay 1 second @18:10:06.50 AFV.select :holdingCoil @18:10:06.61 delay 1 second @18:10:07.63 AS.reconfigure ASnorm @18:10:07.68 AS.seek 0ml @18:10:58.37 AFV.select :carrierSolution @18:10:58.48 delay 2 seconds @18:11:00.50 AS.reconfigure ASconfig @18:11:00.55 AS.seek 1ml @18:11:58.28 delay 1 second @18:11:59.30 AFV.select :holdingCoil @18:11:59.41 delay 1 second @18:12:00.42 ATV.dial :waste @18:12:01.68 AS.reconfigure ASnorm @18:12:01.72 AS.seek 0.5ml @18:12:27.29 ATV.dial :waste2 @18:12:28.63 AS.seek 0ml @18:12:54.14 Full MFB Decontamination @18:12:54.15 Decon extraction column and decon AIV @18:12:54.17 SPE.hold 2:46:40,55C @18:12:54.21 ASV.dial :SPEdelivery @18:12:54.64 delay 20 seconds @18:13:14.67 AFV.select :carrierSolution @18:13:14.78 delay 2 seconds @18:13:16.80 AS.reconfigure ASconfig @18:13:16.85 AS.seek 0.5ml @18:13:45.89 delay 1 second @18:13:46.91 AFV.select :holdingCoil @18:13:47.01 ATV.dial :air1 @18:13:48.46 AS.reconfigure ASslow1 @18:13:48.50 AS.seek 0.52ml @18:13:52.73 ATV.dial :ASV @18:13:54.62 ASV.dial :SPErecovery @18:13:55.29 AS.seek 0.62ml @18:14:15.70 ATV.dial :waste @18:14:17.82 AS.reconfigure ASnorm @18:14:17.87 AS.seek :empty @18:14:49.46 AFV.select :holdingCoil @18:14:49.47 ATV.dial :waste @18:14:49.48 AS.reconfigure ASconfig @18:14:49.53 AS.seek 0ml @18:14:49.81 AFV.select :carrierSolution @18:14:49.92 delay 2 seconds @18:14:51.94 AS.seek 0.5ml @18:15:20.96 delay 1 second @18:15:21.98 AFV.select :holdingCoil @18:15:22.08 ATV.dial :air3 @18:15:22.81 AS.reconfigure ASslow1 @18:15:22.85 AS.seek 0.52ml @18:15:27.14 ATV.dial :air3 @18:15:27.21 AS.seek 0.53ml @18:15:29.50 delay 2 seconds @18:15:31.52 ATV.dial :bleach @18:15:32.01 AS.seek 0.71ml @18:16:08.46 delay 3 seconds @18:16:11.48 ATV.dial :air3 @18:16:12.07 delay 3 seconds @18:16:15.14 AS.seek 0.735ml @18:16:20.42 delay 3 seconds @18:16:23.44 ATV.dial :bleach @18:16:24.07 AS.seek 0.72ml @18:16:27.37 delay 3 seconds @18:16:30.39 ATV.dial ASV @18:16:31.50 ASV.dial :SPErecovery @18:16:31.57 AS.seek 0.365ml @18:17:43.23 ATV.dial :waste @18:17:45.03 AS.reconfigure ASnorm @18:17:45.08 AS.seek :empty @18:18:03.84 ATV.dial :air3 @18:18:04.56 AS.reconfigure ASslow1 @18:18:04.60 AS.seek 0.009ml @18:18:06.93 delay 2 seconds @18:18:08.95 ATV.dial :bleach @18:18:09.42 AS.seek 0.579ml @18:20:04.68 delay 3 seconds @18:20:07.70 ATV.dial :air3 @18:20:08.26 delay 3 seconds @18:20:11.34 AS.seek 0.604ml @18:20:16.65 delay 3 seconds @18:20:19.67 ATV.dial :bleach @18:20:20.27 AS.seek 0.589ml @18:20:23.57 delay 3 seconds @18:20:26.59 ATV.dial :ASV @18:20:27.65 ASV.dial :wasteR @18:20:28.13 AS.seek 0ml @18:22:26.87 delay 1:00 @18:23:26.90 ATV.dial ASV @18:23:26.91 AFV.select :carrierSolution @18:23:27.02 delay 2 seconds @18:23:29.04 AS.reconfigure ASconfig @18:23:29.09 AS.seek 1ml @18:24:26.86 delay 1 second @18:24:27.88 AFV.select :holdingCoil @18:24:27.99 delay 1 second @18:24:29.00 ASV.dial :wasteR @18:24:29.02 AS.reconfigure ASslow1 @18:24:29.07 AS.seek 0ml @18:27:50.46 delay 10 seconds @18:28:00.49 ATV.dial :air3 @18:28:02.06 AS.seek 0.01ml @18:28:04.35 delay 2 seconds @18:28:06.37 ATV.dial :bleach @18:28:06.99 AS.seek 0.58ml @18:30:01.87 delay 3 seconds @18:30:04.89 ATV.dial :air3 @18:30:05.45 delay 3 seconds @18:30:08.53 AS.seek 0.605ml @18:30:13.83 delay 3 seconds @18:30:16.85 ATV.dial :bleach @18:30:17.46 AS.seek 0.59ml @18:30:20.76 delay 3 seconds @18:30:23.78 ATV.dial ASV @18:30:24.86 ASV.dial :SPEdelivery @18:30:25.22 AS.seek 0ml @18:32:24.15 delay 1:00 @18:33:24.17 AFV.select :carrierSolution @18:33:24.28 delay 2 seconds @18:33:26.30 AS.reconfigure ASconfig @18:33:26.35 AS.seek 1ml @18:34:24.09 delay 1 second @18:34:25.11 AFV.select :holdingCoil @18:34:25.22 delay 1 second @18:34:26.23 ATV.dial ASV @18:34:26.24 ASV.dial :SPEdelivery @18:34:26.26 AS.reconfigure ASslow1 @18:34:26.31 AS.seek 0ml @18:37:47.67 delay 10 seconds @18:37:57.69 ATV.dial :air2 @18:37:58.55 AS.reconfigure ASslow0 @18:37:58.59 AS.seek 0.8ml @18:39:19.37 delay 3 seconds @18:39:22.39 ATV.dial :ASV @18:39:23.40 ASV.dial :SPEdelivery @18:39:23.42 AS.reconfigure ASslow1 @18:39:23.46 AS.seek 0.03ml @18:41:58.60 delay 20 seconds @18:42:18.62 ATV.dial :waste,via: :air3 @18:42:20.42 AS.reconfigure ASconfig @18:42:20.46 AS.seek :empty @18:42:22.53 SPE.coast @18:42:22.60 Removing Bleach from Mixing Coil @18:42:22.61 AFV.select :carrierSolution @18:42:22.71 delay 2 seconds @18:42:24.73 AS.seek 0.5ml @18:42:53.82 delay 1 second @18:42:54.84 AFV.select :holdingCoil @18:42:54.94 ATV.dial :ASV @18:42:56.70 ASV.dial :SPErecovery @18:42:57.34 AS.reconfigure ASslow1 @18:42:57.38 AS.seek 0.73ml @18:43:43.92 ASV.dial :wasteD @18:43:44.49 AS.reconfigure ASnorm @18:43:44.53 AS.seek 0ml @18:44:21.65 AFV.select :carrierSolution @18:44:21.76 delay 2 seconds @18:44:23.78 AS.reconfigure ASconfig @18:44:23.83 AS.seek 0.5ml @18:44:52.85 delay 1 second @18:44:53.87 AFV.select :holdingCoil @18:44:53.97 ATV.dial ASV @18:44:53.98 ASV.dial :SPErecovery @18:44:54.62 AS.reconfigure ASslow0 @18:44:54.66 AS.seek 0.3ml @18:45:15.07 ATV.dial :air2 @18:45:16.13 AS.reconfigure ASslow1 @18:45:16.17 AS.seek 0.32ml @18:45:20.51 ATV.dial ASV @18:45:21.57 AS.reconfigure ASslow0 @18:45:21.61 AS.seek 0.57ml @18:45:47.04 ASV.dial :wasteD @18:45:47.63 AS.reconfigure ASnorm @18:45:48.06 AS.seek 0ml @18:46:17.14 AFV.select :carrierSolution @18:46:17.25 delay 2 seconds @18:46:19.27 AS.reconfigure ASconfig @18:46:19.32 AS.seek 0.5ml @18:46:48.35 delay 1 second @18:46:49.37 AFV.select :holdingCoil @18:46:49.47 ATV.dial ASV @18:46:49.48 ASV.dial :SPErecovery @18:46:50.12 AS.reconfigure ASslow0 @18:46:50.17 AS.seek 0.3ml @18:47:10.60 ATV.dial :air2 @18:47:11.65 AS.reconfigure ASslow1 @18:47:11.69 AS.seek 0.32ml @18:47:16.04 ATV.dial ASV @18:47:17.09 AS.reconfigure ASslow0 @18:47:17.13 AS.seek 0.57ml @18:47:42.60 ASV.dial :wasteD @18:47:43.19 AS.reconfigure ASnorm @18:47:43.23 AS.seek 0ml @18:48:12.26 ATV.dial :air2 @18:48:13.26 AS.reconfigure ASslow0 @18:48:13.30 AS.seek 0.3ml @18:48:43.87 ATV.dial :ASV @18:48:44.85 ASV.dial :wasteD @18:48:44.87 AS.reconfigure ASconfig @18:48:44.91 AS.seek 0.05ml @18:48:59.59 ATV.dial :waste @18:49:01.51 AS.seek 0ml @18:49:04.65 ASV.dial :SPEdelivery @18:49:05.73 deconLysateINT @18:49:05.74 ATV.dial :air3 @18:49:06.45 AS.reconfigure ASslow1 @18:49:06.49 AS.seek 0.01ml @18:49:08.85 delay 2 seconds @18:49:10.87 ATV.dial :bleach @18:49:11.32 AS.seek 0.96ml @18:52:22.64 delay 3 seconds @18:52:25.66 ATV.dial :air3 @18:52:26.23 delay 3 seconds @18:52:29.30 AS.seek 0.985ml @18:52:34.69 delay 3 seconds @18:52:37.71 ATV.dial :bleach @18:52:38.32 AS.seek 0.97ml @18:52:41.60 delay 3 seconds @18:52:44.62 ATV.dial AIV @18:52:45.90 AIV.dial :coilToMFB @18:52:45.91 AWV.select :waste @18:52:46.01 AFV.select :carrierSolution @18:52:46.12 delay 2 seconds @18:52:48.14 AS.reconfigure ASconfig @18:52:48.19 AS.seek 1ml @18:52:50.21 delay 1 second @18:52:51.22 AFV.select :holdingCoil @18:52:51.33 delay 1 second @18:52:52.35 AS.reconfigure ASslow0 @18:52:52.39 AS.seek :empty @18:54:33.35 AFV.select :carrierSolution @18:54:33.46 delay 2 seconds @18:54:35.48 AS.reconfigure ASconfig @18:54:35.53 AS.seek 1ml @18:55:33.32 delay 1 second @18:55:34.34 AFV.select :holdingCoil @18:55:34.45 delay 1 second @18:55:35.47 AS.reconfigure ASslow0 @18:55:35.51 AS.seek :empty @18:57:16.51 AFV.select :carrierSolution @18:57:16.62 delay 2 seconds @18:57:18.64 AS.reconfigure ASconfig @18:57:18.69 AS.seek 1ml @18:58:16.48 delay 1 second @18:58:17.50 AFV.select :holdingCoil @18:58:17.61 delay 1 second @18:58:18.63 AS.reconfigure ASslow0 @18:58:18.67 AS.seek :empty @18:59:59.64 ATV.dial :air2 @19:00:00.32 AS.reconfigure ASslow1 @19:00:00.36 AS.seek 0.009ml @19:00:02.75 AS.reconfigure ASslow0 @19:00:02.80 AS.seek 1ml @19:01:42.76 ATV.dial AIV @19:01:43.55 AS.seek 0.02ml @19:03:22.37 ATV.dial :waste @19:03:24.07 AS.seek 0ml @19:03:26.40 AWV.select :air @19:03:26.55 Cleaning PCR @19:03:26.56 AFV.select :carrierSolution @19:03:26.66 delay 2 seconds @19:03:28.68 AS.reconfigure ASconfig @19:03:28.73 AS.seek 0.5ml @19:03:57.76 delay 1 second @19:03:58.78 AFV.select :holdingCoil @19:03:58.88 ASV.dial :waste1 @19:03:59.26 ATV.dial :air3 @19:03:59.96 AS.reconfigure ASslow1 @19:04:00.00 AS.seek 0.51ml @19:04:02.77 delay 2 seconds @19:04:04.79 ATV.dial :bleach @19:04:05.24 AS.seek 0.81ml @19:05:05.84 delay 3 seconds @19:05:08.86 ATV.dial :air3 @19:05:09.42 delay 3 seconds @19:05:12.50 AS.seek 0.835ml @19:05:17.82 delay 3 seconds @19:05:20.84 ATV.dial :bleach @19:05:21.46 AS.seek 0.82ml @19:05:24.73 delay 3 seconds @19:05:27.75 ARV.dial :waste @19:05:28.25 delay 3 seconds @19:05:31.27 ATV.dial :ARV @19:05:32.27 AS.reconfigure ASconfig @19:05:32.31 AS.seek 0.47ml @19:05:52.71 AFV.select :carrierSolution @19:05:52.82 delay 2 seconds @19:05:54.84 AS.seek 1ml @19:06:25.62 delay 1 second @19:06:26.64 AFV.select :holdingCoil @19:06:26.75 delay 1 second @19:06:27.77 AS.reconfigure ASfast @19:06:27.82 AS.seek 0ml @19:06:55.17 AFV.select :carrierSolution @19:06:55.28 delay 2 seconds @19:06:57.30 AS.reconfigure ASconfig @19:06:57.35 AS.seek 1ml @19:07:55.09 delay 1 second @19:07:56.11 AFV.select :holdingCoil @19:07:56.22 delay 1 second @19:07:57.24 AS.reconfigure ASfast @19:07:57.29 AS.seek 0ml @19:08:24.62 AFV.select :carrierSolution @19:08:24.73 delay 2 seconds @19:08:26.75 AS.reconfigure ASconfig @19:08:26.80 AS.seek 1ml @19:09:24.60 delay 1 second @19:09:25.62 AFV.select :holdingCoil @19:09:25.73 delay 1 second @19:09:26.75 AS.reconfigure ASfast @19:09:26.80 AS.seek 0ml @19:09:54.14 ARV.dial between carrier and air1 @19:09:54.64 ATV.dial :air3 @19:09:55.93 AS.reconfigure ASslow1 @19:09:55.97 AS.seek 0.01ml @19:09:58.29 delay 2 seconds @19:10:00.31 ATV.dial :bleach @19:10:00.91 AS.seek 0.81ml @19:12:42.21 delay 3 seconds @19:12:45.23 ATV.dial :air3 @19:12:45.79 delay 3 seconds @19:12:48.87 AS.seek 0.835ml @19:12:54.18 delay 3 seconds @19:12:57.20 ATV.dial :bleach @19:12:57.82 AS.seek 0.82ml @19:13:01.12 delay 3 seconds @19:13:04.14 ATV.dial PCR @19:13:04.54 AS.reconfigure ASconfig @19:13:04.58 AS.seek :empty @19:13:52.04 PCR.program :bleachProfile @19:13:52.09 PCR program should complete in < 2:45 @19:13:52.10 PCR.run 10:00 @19:16:53.90 PCR.off @19:16:53.93 AFV.select :carrierSolution @19:16:54.04 delay 2 seconds @19:16:56.06 AS.seek 1ml @19:17:53.87 delay 1 second @19:17:54.89 AFV.select :holdingCoil @19:17:55.00 delay 1 second @19:17:56.02 AS.reconfigure ASfast @19:17:56.07 AS.seek 0ml @19:18:23.40 delay 3 seconds @19:18:26.42 AFV.select :carrierSolution @19:18:26.53 delay 2 seconds @19:18:28.55 AS.reconfigure ASconfig @19:18:28.61 AS.seek 1ml @19:19:26.34 delay 1 second @19:19:27.36 AFV.select :holdingCoil @19:19:27.47 delay 1 second @19:19:28.49 AS.reconfigure ASfast @19:19:28.54 AS.seek 0ml @19:19:55.84 delay 3 seconds @19:19:58.86 AFV.select :carrierSolution @19:19:58.97 delay 2 seconds @19:20:00.99 AS.reconfigure ASconfig @19:20:01.05 AS.seek 1ml @19:20:58.78 delay 1 second @19:20:59.80 AFV.select :holdingCoil @19:20:59.91 delay 1 second @19:21:00.93 AS.reconfigure ASfast @19:21:00.98 AS.seek 0ml @19:21:28.32 delay 3 seconds @19:21:31.34 AFV.select :carrierSolution @19:21:31.45 delay 2 seconds @19:21:33.47 AS.reconfigure ASconfig @19:21:33.53 AS.seek 1ml @19:22:31.67 delay 1 second @19:22:32.69 AFV.select :holdingCoil @19:22:32.80 delay 1 second @19:22:33.82 AS.reconfigure ASfast @19:22:33.87 AS.seek 0ml @19:23:01.39 delay 3 seconds @19:23:04.41 AFV.select :carrierSolution @19:23:04.52 delay 2 seconds @19:23:06.54 AS.reconfigure ASconfig @19:23:06.60 AS.seek 1ml @19:24:04.34 delay 1 second @19:24:05.36 AFV.select :holdingCoil @19:24:05.47 delay 1 second @19:24:06.49 AS.reconfigure ASnorm @19:24:06.54 AS.seek 0ml @19:24:57.24 AFV.select :carrierSolution @19:24:57.35 delay 2 seconds @19:24:59.37 AS.reconfigure ASconfig @19:24:59.42 AS.seek 1ml @19:25:57.17 delay 1 second @19:25:58.19 AFV.select :holdingCoil @19:25:58.30 delay 1 second @19:25:59.31 ATV.dial :waste @19:26:00.57 AS.reconfigure ASnorm @19:26:00.61 AS.seek 0.5ml @19:26:26.15 ATV.dial :waste2 @19:26:27.47 AS.seek 0ml @19:26:53.03 Starting mfb @19:26:53.04 pcrBAC sampling at most 100ml, wcr at most 100ml @19:26:53.09 BACfans.email "Started mfb in tube 2 at 19:26:53CEST15-May-19\n{}\nCan@19:26:08, 22.3C, 26% humidity, 14.8psia, 11.982V, 0.701A, 0.701A avg, 8.40W",Subject: "pcrBAC sampling at most 100ml, wcr at most 100ml" @19:26:53.14
Loading sh1 puck from tube 2 into Collection Clamp @19:26:53.22 Hand.open @19:26:54.16 SC.reconfigure SCloose @19:26:54.32 Elbow.reconfigure TightElbow @19:26:54.37 Elbow.seek 2 @19:26:54.91 Forearm.seek 2 + 700 counts @19:26:55.96 SE.reconfigure SEpick @19:26:56.03 SE.seek beamBreak @19:26:59.09 SE.configure SEconfig @19:26:59.25 Elbow.seek 2 @19:26:59.44 Forearm.seek 2 + 300 counts @19:26:59.62 Forearm.reconfigure LooseForearm @19:26:59.67 Hand.close @19:27:00.44 SE.seek :down @19:27:00.63
Forearm.seek :retract @19:27:01.56 Elbow.seek CC @19:27:03.18 SE.coast @19:27:03.32
Elbow.reconfigure LooseElbow @19:27:03.41 Forearm.reconfigure TightForearm @19:27:03.47 Forearm.seek CC @19:27:05.19 CC.seek :closed @19:27:09.53 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.33 @19:27:09.54 Hand.open @19:27:10.35 Forearm.reconfigure LooseForearm @19:27:10.40 Forearm.seek :retract @19:27:11.40 SC.coast @19:27:11.43 collectSample 100ml w/bubble point of 55psi @19:27:11.45 Priming 7ml Gate.power :sampler,:ON @19:27:11.46 Intake.select :close @19:27:11.47 Exhaust.select :close @19:27:11.48 SS.hold @19:27:11.68 Calibrating pressure and temperature sensors @19:27:11.69 CTV.dial :air @19:27:11.71 SSV.dial :spit,avoiding: :exhaust @19:27:11.72 SS.reconfigure SSfast @19:27:11.77 SS.seek 5ml @19:27:14.70 SSV.dial CBV @19:27:14.73
CBV.dial SSV @19:27:16.25 delay 3.8 seconds @19:27:20.18 SSV.dial :spit @19:27:21.69 SS.seek 0ml @19:27:24.66 SSV.dial CBV @19:27:26.16 CTV.dial :intake @19:27:27.02 Waiting for external sampler to finish priming... @19:27:27.03 delayUntil "19:32:11.46" @19:32:11.48 SSV.dial CBV @19:32:11.50
CBV.dial SSV @19:32:11.51 Allowing 40s for 7.0ml of remaining 7.0ml @19:32:11.52 Intake.select :open @19:32:13.68 Intake @15.8psia, vacuum from 9.3 to 13.3psi @19:32:13.70 SS.reconfigure SSinhale.with maxSpeed: 150 @19:32:13.74 SS.seek 7ml @19:32:19.46 Stroke completed -- waiting for pressure to equalize ... @19:32:35.23 Intake.select :close @19:32:35.38 SSV.dial :exhaust @19:32:36.24 Exhaust.select :open @19:32:36.35 SS.reconfigure SSexhale @19:32:36.40 SS.seek 0ml @19:32:40.67 Exhaust.select :close @19:32:41.22 Sampling 100.0ml @19:32:41.24 Intake.select :close @19:32:41.25 Exhaust.select :close @19:32:41.26 SS.reconfigure SSconfig @19:32:41.30 SS.hold @19:32:41.49 CTV.dial :intake @19:32:41.50 SSV.dial CBV @19:32:41.53
CBV.dial SSV @19:32:42.41 Allowing 130s for 25.0ml of remaining 100.0ml @19:32:42.42 Intake.select :open @19:32:44.58 Intake @15.8psia, vacuum from 9.3 to 13.3psi @19:32:44.59 SS.reconfigure SSinhale.with maxSpeed: 150 @19:32:44.64 SS.seek 25ml @19:32:48.30 Vacuum too high -- waiting up to 40s @19:32:53.67 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:32:53.68 SS.reconfigure SSinhale.with maxSpeed: 135 @19:32:53.73 SS.seek 25ml @19:32:56.89 Vacuum too high -- waiting up to 40s @19:33:02.49 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:33:02.51 SS.reconfigure SSinhale.with maxSpeed: 122 @19:33:03.00 SS.seek 25ml @19:33:06.17 Vacuum too high -- waiting up to 40s @19:33:11.55 SS.reconfigure SSinhale.with maxSpeed: 110 @19:33:11.59 SS.seek 25ml @19:33:14.61 Vacuum too high -- waiting up to 40s @19:33:19.75 SS.reconfigure SSinhale.with maxSpeed: 99 @19:33:19.79 SS.seek 25ml @19:33:22.83 Vacuum too high -- waiting up to 40s @19:33:27.97 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:33:27.99 SS.reconfigure SSinhale.with maxSpeed: 89 @19:33:28.03 SS.seek 25ml @19:33:31.73 Vacuum too high -- waiting up to 40s @19:33:36.86 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:33:36.87 SS.reconfigure SSinhale.with maxSpeed: 80 @19:33:36.92 SS.seek 25ml @19:33:41.11 Vacuum too high -- waiting up to 40s @19:33:45.78 SS.reconfigure SSinhale.with maxSpeed: 72 @19:33:46.28 SS.seek 25ml @19:33:46.78 Stroke completed -- waiting for pressure to equalize ... @19:33:48.66 Intake.select :close @19:33:48.81 SSV.dial :exhaust @19:33:49.67 Exhaust.select :open @19:33:49.78 SS.reconfigure SSexhale @19:33:49.83 SS.seek 0ml @19:33:59.33 Exhaust.select :close @19:33:59.44 SSV.dial CBV @19:33:59.46
CBV.dial SSV @19:34:00.33 Allowing 130s for 25.0ml of remaining 75.0ml @19:34:00.34 Intake.select :open @19:34:02.50 Intake @15.7psia, vacuum from 9.2 to 13.2psi @19:34:02.51 SS.reconfigure SSinhale.with maxSpeed: 72 @19:34:02.56 SS.seek 25ml @19:34:06.89 Vacuum too high -- waiting up to 40s @19:34:10.86 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:34:10.87 SS.reconfigure SSinhale.with maxSpeed: 65 @19:34:10.92 SS.seek 25ml @19:34:15.08 Vacuum too high -- waiting up to 40s @19:34:19.07 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:34:19.09 SS.reconfigure SSinhale.with maxSpeed: 59 @19:34:19.13 SS.seek 25ml @19:34:24.12 Vacuum too high -- waiting up to 40s @19:34:28.08 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:34:28.10 SS.reconfigure SSinhale.with maxSpeed: 53 @19:34:28.14 SS.seek 25ml @19:34:34.61 Vacuum too high -- waiting up to 40s @19:34:39.28 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:34:39.29 SS.reconfigure SSinhale.with maxSpeed: 48 @19:34:39.34 SS.seek 25ml @19:34:46.42 Vacuum too high -- waiting up to 40s @19:34:50.63 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:34:50.64 SS.reconfigure SSinhale.with maxSpeed: 43 @19:34:50.69 SS.seek 25ml @19:34:59.95 Vacuum too high -- waiting up to 40s @19:35:04.39 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:35:04.41 SS.reconfigure SSinhale.with maxSpeed: 39 @19:35:04.45 SS.seek 25ml @19:35:05.75 Stroke completed -- waiting for pressure to equalize ... @19:35:08.24 Intake.select :close @19:35:08.38 SSV.dial :exhaust @19:35:09.24 Exhaust.select :open @19:35:09.35 SS.reconfigure SSexhale @19:35:09.40 SS.seek 0ml @19:35:19.06 Exhaust.select :close @19:35:19.17 SSV.dial CBV @19:35:19.19
CBV.dial SSV @19:35:20.08 Allowing 130s for 25.0ml of remaining 50.0ml @19:35:20.09 Intake.select :open @19:35:22.25 Intake @15.8psia, vacuum from 9.3 to 13.3psi @19:35:22.26 SS.reconfigure SSinhale.with maxSpeed: 39 @19:35:22.31 SS.seek 25ml @19:35:33.54 Vacuum too high -- waiting up to 40s @19:35:36.82 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:35:36.83 SS.reconfigure SSinhale.with maxSpeed: 35 @19:35:36.88 SS.seek 25ml @19:35:52.64 Vacuum too high -- waiting up to 40s @19:35:56.15 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:35:56.17 SS.reconfigure SSinhale.with maxSpeed: 32 @19:35:56.21 SS.seek 25ml @19:36:21.94 Stroke completed -- waiting for pressure to equalize ... @19:36:27.48 Intake.select :close @19:36:27.63 SSV.dial :exhaust @19:36:28.49 Exhaust.select :open @19:36:28.60 SS.reconfigure SSexhale @19:36:28.65 SS.seek 0ml @19:36:38.20 Exhaust.select :close @19:36:38.31 SSV.dial CBV @19:36:38.33
CBV.dial SSV @19:36:39.20 Allowing 130s for 25.0ml of remaining 25.0ml @19:36:39.21 Intake.select :open @19:36:41.37 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:36:41.39 SS.reconfigure SSinhale.with maxSpeed: 32 @19:36:41.43 SS.seek 25ml @19:37:27.54 Vacuum too high -- waiting up to 40s @19:37:32.21 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:37:32.23 SS.reconfigure SSinhale.with maxSpeed: 29 @19:37:32.27 SS.seek 25ml @19:37:42.73 Stroke completed -- waiting for pressure to equalize ... @19:37:47.03 Intake.select :close @19:37:47.17 SSV.dial :exhaust @19:37:48.04 Exhaust.select :open @19:37:48.15 SS.reconfigure SSexhale @19:37:48.20 SS.seek 0ml @19:37:57.41 Exhaust.select :close @19:37:57.52 Sampled 100.0ml @19:37:57.54 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @19:37:57.60 CSV.dial :waste @19:37:57.65 CS.seek 0ml @19:37:57.74 SSV.dial CBV,avoiding: :exhaust @19:37:57.77 CSV.dial :air @19:37:57.80
CBV.dial SSV @19:37:58.69 CS.seek 10ml @19:37:59.95
CTV.dial :air @19:38:00.52 Gate.power :sampler,:OFF @19:38:00.81
delay 15 seconds @19:38:15.83 SSV.dial :spit,avoiding: :exhaust @19:38:17.33 CTV.dial CSV,avoiding: :intake @19:38:17.37
SS.seek 0ml @19:38:17.49 SSV.dial CBV,avoiding: :exhaust @19:38:26.48 CBV.dial between SSV and waste2 @19:38:26.90 CTV.dial CSV,avoiding: :air @19:38:26.93
CSV.dial CTV @19:38:27.43 CS.reconfigure CSpressurize @19:38:27.47 CS.seek 0ml @19:38:53.92 CSV.dial :air @19:38:54.46 CS.reconfigure CSfast @19:38:54.50 CS.seek 10ml @19:39:21.39 CSV.dial between air and CTV @19:39:21.76 CS.seek 2.611ml @19:39:41.83 CTV.dial CSV,avoiding: :air @19:39:41.86
CSV.dial CTV @19:39:42.19 CS.reconfigure CSpressurize @19:39:42.23 CS.seek 0ml @19:39:46.55 BubblePoint: Intake @70.1psia, Outlet @14.8psia CBV.dial SSV @19:39:46.92 SS.reconfigure SSconfig @19:39:46.96 SS.seek 10ml @19:39:52.81 delay 15 seconds @19:40:07.93 Evacuated: Intake @38.6psia, Outlet @11.6psia @19:40:07.94 SSV.dial :spit,avoiding: :exhaust @19:40:09.45 SS.reconfigure SSexhale @19:40:09.49 SS.seek 0ml @19:40:14.66 SS.reconfigure SSfast @19:40:14.71 SS.seek 5ml @19:40:17.55 SSV.dial CBV,avoiding: :exhaust @19:40:17.58
CBV.dial SSV @19:40:19.09 CTV.dial :air,avoiding: :intake @19:40:20.62 CSV.dial CTV @19:40:20.66
SS.seek 10ml @19:40:21.65 CSV.dial :air @19:40:22.19 CS.reconfigure CSfast @19:40:22.24 CS.seek 0ml @19:40:23.14
SSV.dial :spit,avoiding: :exhaust @19:40:24.65 SS.reconfigure SSexhale @19:40:24.69 SS.seek 0ml @19:40:26.27 CS.coast @19:40:29.83
SS.reconfigure SSfast @19:40:29.88 SS.seek 5ml @19:40:32.70 SSV.dial CBV,avoiding: :exhaust @19:40:32.73
CBV.dial SSV @19:40:35.24 SSV.dial :spit,avoiding: :exhaust @19:40:36.76 SS.reconfigure SSconfig @19:40:36.80 SS.seek 0ml @19:40:40.05 SS.coast @19:40:40.09 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @19:40:40.16 CSV.dial :waste @19:40:40.27 SSV.dial CBV,avoiding: :exhaust @19:40:40.29
CBV.dial SSV @19:40:41.04 CS.seek 0ml @19:40:41.10 CSV.dial :air @19:40:41.83
CTV.dial :air @19:40:41.84 delay 15 seconds @19:40:42.38 CS.seek 10ml @19:40:56.86
SSV.dial :spit,avoiding: :exhaust @19:40:58.38 CTV.dial CSV,avoiding: :intake @19:40:58.41
SS.reconfigure SSexhale @19:40:58.50 SS.seek 0ml @19:40:58.69 SSV.dial CBV,avoiding: :exhaust @19:41:09.23 CBV.dial between SSV and waste2 @19:41:09.65 CSV.dial between air and CTV @19:41:10.01 CS.seek 8.279ml @19:41:15.36 CTV.dial CSV,avoiding: :air @19:41:15.39
CSV.dial CTV @19:41:15.73 CS.reconfigure CSpressurize @19:41:15.77 CS.seek 0ml @19:41:37.61 CSV.dial :air @19:41:38.13 CS.reconfigure CSfast @19:41:38.17 CS.seek 10ml @19:42:05.01 CSV.dial between air and CTV @19:42:05.37 CS.seek 3.163ml @19:42:24.14 CTV.dial CSV,avoiding: :air @19:42:24.17
CSV.dial CTV @19:42:24.51 CS.reconfigure CSpressurize @19:42:24.55 CS.seek 0ml @19:42:32.93 BubblePoint: Intake @69.8psia, Outlet @14.4psia CBV.dial SSV @19:42:33.31 SS.reconfigure SSconfig @19:42:33.35 SS.seek 10ml @19:42:38.72 delay 15 seconds @19:42:53.84 Evacuated: Intake @41.2psia, Outlet @15.4psia @19:42:53.85 SSV.dial :spit,avoiding: :exhaust @19:42:55.37 SS.reconfigure SSexhale @19:42:55.41 SS.seek 0ml @19:43:00.49 SS.reconfigure SSfast @19:43:00.54 SS.seek 5ml @19:43:03.56 SSV.dial CBV,avoiding: :exhaust @19:43:03.59
CBV.dial SSV @19:43:05.10 CTV.dial :air,avoiding: :intake @19:43:06.64 CSV.dial CTV @19:43:06.68
SS.seek 10ml @19:43:07.67 CSV.dial :air @19:43:08.19 CS.reconfigure CSfast @19:43:08.24 CS.seek 0ml @19:43:09.41
SSV.dial :spit,avoiding: :exhaust @19:43:09.73 CS.coast @19:43:10.92
SS.reconfigure SSexhale @19:43:10.96 SS.seek 0ml @19:43:16.14 SS.reconfigure SSfast @19:43:16.20 SS.seek 5ml @19:43:19.05 SSV.dial CBV,avoiding: :exhaust @19:43:19.08
CBV.dial SSV @19:43:21.59 SSV.dial :spit,avoiding: :exhaust @19:43:23.10 SS.reconfigure SSconfig @19:43:23.14 SS.seek 0ml @19:43:26.36 SS.coast @19:43:26.39 Lysing: Adding 1.5ml lysis @19:43:26.40 CBV.dial between CSV and waste1,avoiding: :DBV @19:43:28.12 CTV.dial CSV @19:43:29.64 CSV.dial CSR @19:43:30.97 Pulling 1ml of CSR.lysis CSR.select :lysis @19:43:31.08 CS.reconfigure CSconfig @19:43:31.13 CS.seek 1ml @19:43:34.67 delay 5 seconds @19:43:39.69 CSR.select :air @19:43:39.86 CS.seek 6ml @19:43:53.55 delay 2 seconds @19:43:55.57 CSV.dial :waste1 @19:43:57.10 CS.reconfigure CSfast @19:43:57.14 CS.seek :empty @19:44:13.45 CSV.dial CSR @19:44:15.01 Pulling 1.5ml of CSR.lysis CSR.select :lysis @19:44:15.12 CS.reconfigure CSconfig @19:44:15.17 CS.seek 1.5ml @19:44:20.05 delay 5 seconds @19:44:25.07 CSR.select :air @19:44:25.23 CS.seek 4.5ml @19:44:33.89 delay 2 seconds @19:44:35.91 CSV.dial CTV @19:44:36.43 CS.seek 1.275ml @19:44:46.03 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @19:44:46.04 CH.seek 85C,8:00 @19:49:49.48 CH.hold 5:00,85C @19:54:49.62 Collection Puck Heater at 84C after lysing @19:54:49.64 CS.seek 0ml @19:54:54.08 CTV.dial between spare and air,avoiding: :intake @19:54:55.28 CSV.dial :air @19:54:55.81 CS.seek 5ml @19:55:09.62 CSV.dial CTV @19:55:10.13 delay 2 seconds @19:55:12.15 CSV.dial :air @19:55:12.66 delay 2 seconds @19:55:14.68 CSV.dial CTV @19:55:15.21 delay 2 seconds @19:55:17.23 CSV.dial :air @19:55:18.22 delay 2 seconds @19:55:20.25 CS.seek 0ml @19:55:34.05 Recovering Lysate into Collection syringe @19:55:34.06 CSV.dial CBV @19:55:35.26 CBV.dial CSV @19:55:35.68 CS.seek 5ml @19:55:49.52 delay 5 seconds @19:55:54.54 CTV.dial :air @19:55:55.13 CS.seek 7ml @19:56:01.30 delay 5 seconds @19:56:06.32 CSV.dial :air2 @19:56:06.83 CS.seek 10ml @19:56:15.52 CSV.dial between air and CTV,avoiding: CBV @19:56:16.88 Lysing Complete @19:56:16.94 Storing sh1 puck in Collection Clamp to tube 1 @19:56:17.10 SC.reconfigure SCtight @19:56:17.14 SC.seek 1 @19:56:19.23 SC.reconfigure SCloose @19:56:19.43 Elbow.reconfigure TightElbow @19:56:19.47 Elbow.seek 1 @19:56:21.39 Forearm.reconfigure TightForearm @19:56:21.43 Forearm.seek 1 + 700 counts @19:56:22.45 SE.reconfigure SEpick @19:56:22.52 SE.seek beamBreak @19:56:25.47 SE.configure SEconfig @19:56:25.52 SE.seek :place @19:56:26.10 Forearm.reconfigure LooseForearm @19:56:26.13 Forearm.seek :retract @19:56:27.06 Elbow.seek CC @19:56:28.70 Elbow.reconfigure LooseElbow @19:56:28.79 Forearm.seek CC @19:56:29.86 Hand.close @19:56:30.59 CC.seek :open @19:56:35.06 Forearm.seek :retract @19:56:36.09 Elbow.reconfigure TightElbow @19:56:36.13 Elbow.seek 1 @19:56:38.12 Elbow.reconfigure LimpElbow @19:56:38.17 Forearm.seek 1 @19:56:39.20 Elbow.hold @19:56:39.26 Elbow.reconfigure TightElbow @19:56:39.30 Hand.open @19:56:40.20 Forearm.force -20 @19:56:40.23 Forearm.seek 1 + 348 counts @19:56:40.54 Forearm.reconfigure TightForearm @19:56:40.58 Forearm.seek 1 + 700 counts @19:56:40.94 SE.seek :down @19:56:42.44
Forearm.reconfigure LooseForearm @19:56:42.54 Forearm.seek :retract @19:56:43.75 SE.coast @19:56:43.78
SC.coast @19:56:43.82 Tranferring lysate to PRV CSV.dial :air @19:56:44.16 CS.seek 10ml @19:56:44.24 CSV.dial between air and CTV @19:56:44.60 AIV.dial :bypass @19:56:44.62 Beginning samplePassC @19:56:44.63 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:56:44.69 Hand.open @19:56:45.07 Elbow.seek FlushPuck::Garage @19:56:47.11 Elbow.reconfigure LooseElbow @19:56:47.20 Forearm.seek :garage @19:56:48.25 Hand.close @19:56:49.07 Forearm.seek :retract @19:56:50.10 Elbow.reconfigure TightElbow @19:56:50.14 Elbow.seek CC @19:56:53.06 Elbow.reconfigure LooseElbow @19:56:53.14 Forearm.reconfigure TightForearm @19:56:53.18 Forearm.seek CC @19:56:54.47 CC.seek :closed @19:56:58.82 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.39 @19:56:58.89 Hand.open @19:56:59.64 Forearm.reconfigure LooseForearm @19:56:59.68 Forearm.seek :retract @19:57:00.70 AWV.select :air @19:57:00.71 AIV.dial :bypass @19:57:00.72 PSV.dial PSR @19:57:02.34 Pulling 1.5ml of PSR.flush @19:57:02.35 PSR.select :flush @19:57:02.48 PS.seek 1.5ml @19:57:18.71 delay 5 seconds @19:57:23.73 PSV.dial :waste1 @19:57:24.27 PS.seek 0ml @19:57:40.62 PSV.dial PSR @19:57:41.14 PSR.select :air @19:57:41.25 PS.seek 2.5ml @19:58:07.81 PRV.dial CBV @19:58:07.84
CBV.dial PRV @19:58:08.76 PSV.dial :mixloop @19:58:09.90 CTV.dial :air,avoiding: :intake @19:58:09.91 PTV.dial :PRVmixing @19:58:10.01 PS.seek 0ml @19:58:36.71 PSV.dial :air @19:58:37.22 PS.seek 2.5ml @19:59:03.81 PSV.dial :mixloop @19:59:04.32 PS.seek 0ml @19:59:30.93 PSV.dial :air @19:59:31.44 PS.seek 2.5ml @19:59:58.09 PSV.dial :mixloop @19:59:58.60 PS.seek 0ml @20:00:25.00 Garaging FlushPuck in Collection Clamp @20:00:25.52 Elbow.reconfigure TightElbow @20:00:25.56 Elbow.seek CC @20:00:25.83 Elbow.reconfigure LooseElbow @20:00:25.92 Forearm.seek CC @20:00:26.99 Hand.close @20:00:27.72 CC.seek :open @20:00:32.07 Forearm.seek :retract @20:00:33.08 Elbow.reconfigure TightElbow @20:00:33.13 Elbow.seek :garage @20:00:36.28 Elbow.reconfigure LooseElbow @20:00:36.36 Forearm.reconfigure TightForearm @20:00:36.41 Forearm.seek :garage @20:00:37.55 Hand.open @20:00:38.39 Forearm.reconfigure LooseForearm @20:00:38.43 Forearm.seek :retract @20:00:39.52 Connecting PS to CS to transfer sample @20:00:39.53 CBV.dial AIV @20:00:39.54 AIV.dial :bypass @20:00:39.55 PSV.dial :mixloop @20:00:39.56 PTV.dial :PRVmixing @20:00:39.57 PRV.dial AIV @20:00:39.58 CTV.dial :air,avoiding: :intake @20:00:39.60 Loading lyfil puck from tube 2 into Collection Clamp @20:00:39.81 SC.reconfigure SCtight @20:00:39.85 SC.seek 2 @20:00:42.22 SC.reconfigure SCloose @20:00:42.41 Elbow.reconfigure TightElbow @20:00:42.45 Elbow.seek 2 @20:00:44.47 Forearm.reconfigure TightForearm @20:00:44.51 Forearm.seek 2 + 700 counts @20:00:45.55 SE.reconfigure SEpick @20:00:45.62 SE.seek beamBreak @20:00:48.69 SE.configure SEconfig @20:00:48.83 Elbow.seek 2 @20:00:49.05 Forearm.seek 2 + 300 counts @20:00:49.22 Forearm.reconfigure LooseForearm @20:00:49.26 Hand.close @20:00:49.99 SE.seek :down @20:00:50.18
Forearm.seek :retract @20:00:51.11 Elbow.seek CC @20:00:52.70 Elbow.reconfigure LooseElbow @20:00:52.78 Forearm.reconfigure TightForearm @20:00:52.84 Forearm.seek CC @20:00:52.86 SE.coast @20:00:54.23
CC.seek :closed @20:00:58.52 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.39 @20:00:58.53 Hand.open @20:00:59.27 Forearm.reconfigure LooseForearm @20:00:59.31 Forearm.seek :retract @20:01:00.36 SC.coast @20:01:00.39 CTV.dial CSV,avoiding: :intake @20:01:02.40 CSV.dial CTV @20:01:02.74 CS.seek 5ml @20:01:16.59 delay 1:20 @20:02:36.62 CS.seek 2.5ml @20:02:44.15 delay 40 seconds @20:03:24.18 CS.seek 0ml @20:03:31.68 delay 1:00 @20:04:31.70 PSV.dial :air @20:04:32.21 PS.seek 2.5ml @20:04:58.40 PSV.dial :mixloop @20:04:58.90 delay 3 seconds @20:05:01.92 PSV.dial :air @20:05:02.40 delay 2 seconds @20:05:04.42 PSV.dial :mixloop @20:05:04.90 delay 3 seconds @20:05:07.92 PSV.dial :waste1 @20:05:09.40 PS.seek :empty @20:05:35.53 CBV.dial :waste1 @20:05:36.06 delay 5 seconds @20:05:41.08 CBV.dial AIV @20:05:41.62 delay 10 seconds @20:05:51.65 CBV.dial :waste1 @20:05:52.18 delay 10 seconds @20:06:02.21 CBV.dial AIV @20:06:02.75 delay 10 seconds @20:06:12.77 CTV.dial CSV,avoiding: :intake @20:06:12.79 CBV.dial between AIV and waste1 @20:06:13.15 CSV.dial :air @20:06:13.81 CS.seek 10ml @20:06:40.72 CSV.dial CTV @20:06:41.23 CS.seek 0ml @20:07:08.56 CBV.dial :waste1 @20:07:08.90 delay 10 seconds @20:07:18.93 CTV.dial :air,avoiding: :intake @20:07:20.54 Storing lyfil puck in Collection Clamp to tube 1 @20:07:20.69 SC.reconfigure SCtight @20:07:20.74 SC.seek 1 @20:07:22.88 SC.reconfigure SCloose @20:07:23.07 Elbow.reconfigure TightElbow @20:07:23.11 Elbow.seek 1 @20:07:25.00 Forearm.reconfigure TightForearm @20:07:25.05 Forearm.seek 1 + 700 counts @20:07:26.07 SE.reconfigure SEpick @20:07:26.14 SE.seek beamBreak @20:07:29.25 SE.configure SEconfig @20:07:29.39 Elbow.reconfigure LimpElbow @20:07:29.43 Forearm.reconfigure LooseForearm @20:07:29.47 Forearm.seek 1 @20:07:29.79 Elbow.hold @20:07:29.85 Elbow.reconfigure TightElbow @20:07:29.95 SE.seek 0.06 Pucks below Top Plate @20:07:30.39 Forearm.force -20 @20:07:30.42 Forearm.seek 1 + 335 counts @20:07:30.72 Forearm.reconfigure TightForearm @20:07:30.76 Forearm.seek 1 + 700 counts @20:07:31.04 SE.seek 1.06 Pucks below Top Plate @20:07:31.73 Forearm.reconfigure LooseForearm @20:07:31.77 Forearm.seek :retract @20:07:32.68 Elbow.seek CC @20:07:34.37 Elbow.reconfigure LooseElbow @20:07:34.45 Forearm.seek CC @20:07:35.51 Hand.close @20:07:36.21 CC.seek :open @20:07:40.72 Forearm.seek :retract @20:07:41.78 Elbow.reconfigure TightElbow @20:07:41.83 Elbow.seek 1 @20:07:43.73 Elbow.reconfigure LimpElbow @20:07:43.78 Forearm.seek 1 @20:07:44.82 Elbow.hold @20:07:44.88 Elbow.reconfigure TightElbow @20:07:44.93 Hand.open @20:07:45.84 Forearm.force -20 @20:07:45.87 Forearm.seek 1 + 346 counts @20:07:46.18 Forearm.reconfigure TightForearm @20:07:46.22 Forearm.seek 1 + 700 counts @20:07:46.52 SE.seek :down @20:07:47.65
Forearm.reconfigure LooseForearm @20:07:47.69 Forearm.seek :retract @20:07:49.25 SE.coast @20:07:49.28
SC.coast @20:07:49.31 PSV.dial :air @20:07:50.51 PS.seek 0.499ml @20:07:56.40 delay 3 seconds @20:07:59.42 PSV.dial :mixloop @20:07:59.89 PRV.dial :air @20:08:00.39 delay 3 seconds @20:08:03.41 PRV.dial CBV @20:08:03.92 CBV.dial AIV @20:08:04.45 PRV.dial between CBV and air @20:08:04.79 PRV.dial :air @20:08:05.18 PS.reconfigure PSslow @20:08:05.22 PS.seek 0.599ml @20:08:07.69 PRV.dial :waste2 @20:08:08.51 PS.reconfigure PSconfig @20:08:08.55 PS.seek :empty @20:08:15.50 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:08:15.56 Hand.open @20:08:15.95 Elbow.seek FlushPuck::Garage @20:08:17.93 Elbow.reconfigure LooseElbow @20:08:18.01 Forearm.seek :garage @20:08:19.04 Hand.close @20:08:19.95 Forearm.seek :retract @20:08:21.01 Elbow.reconfigure TightElbow @20:08:21.05 Elbow.seek CC @20:08:23.95 Elbow.reconfigure LooseElbow @20:08:24.04 Forearm.reconfigure TightForearm @20:08:24.08 Forearm.seek CC @20:08:25.37 CC.seek :closed @20:08:30.06 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.42 @20:08:30.16 Hand.open @20:08:30.90 Forearm.reconfigure LooseForearm @20:08:30.94 Forearm.seek :retract @20:08:32.04 Clearing PML for modified lysate @20:08:32.05 CTV.dial :air,avoiding: :intake @20:08:32.06 PRV.dial :waste2 @20:08:32.07 PTV.dial :PRV,avoiding: :puck @20:08:32.90 delay 2 seconds @20:08:34.92 PTV.dial :PRVmixing,avoiding: :puck @20:08:35.75 delay 2 seconds @20:08:37.77 PSV.dial :PML @20:08:37.79 delay 2 seconds @20:08:39.80 PRV.dial :air @20:08:40.62 delay 2 seconds @20:08:42.70 PS.seek 1.499ml @20:08:58.94 delay 5 seconds @20:09:03.97 PS.seek 2.5ml @20:09:14.90 PSV.dial :air @20:09:15.52 PS.seek 0ml @20:09:41.75 mixing lysate with speDil @20:09:41.76 PSV.dial :PML @20:09:42.23 CBV.dial :PRV @20:09:42.24 PRV.dial :air @20:09:42.25 delay 2 seconds @20:09:44.27 PRV.dial :speDil @20:09:44.78 delay 2 seconds @20:09:46.86 PS.reconfigure PSslow1 @20:09:46.91 PS.seek 0.225ml @20:10:11.12 delay 5 seconds @20:10:16.14 PRV.dial CBV @20:10:17.00 delay 2 seconds @20:10:19.08 PS.seek 0.475ml @20:10:45.19 delay 5 seconds @20:10:50.21 PRV.dial :air @20:10:50.73 delay 2 seconds @20:10:52.76 PS.seek 0.965ml @20:11:43.03 delay 10 seconds @20:11:53.05 PRV.dial CBV @20:11:53.55 CTV.dial :air,avoiding: :intake @20:11:53.56 PSV.dial :air @20:11:54.06 PS.reconfigure PSconfig @20:11:54.10 PS.seek 2.5ml @20:12:10.69 delay 3 seconds @20:12:13.71 PSV.dial PTV @20:12:14.55 PTV.dial :PRV,avoiding: :puck @20:12:15.37 PS.seek 0ml @20:12:41.53 PSV.dial :air @20:12:42.37 PS.seek 2.5ml @20:13:08.56 delay 3 seconds @20:13:11.58 PSV.dial PTV @20:13:12.51 PTV.dial :PRV,avoiding: :puck @20:13:12.53 PS.seek 0ml @20:13:38.72 Pushing modified lysate into AIV Injection Loop @20:13:38.73 CBV.dial AIV @20:13:38.74 AIV.dial :bypass @20:13:38.75 PRV.dial CBV @20:13:38.76 PSV.dial :air @20:13:39.57 PS.seek 2.5ml @20:14:05.94 delay 3 seconds @20:14:08.96 PSV.dial :PML @20:14:09.45 PTV.dial :PRVmixing,avoiding: :puck @20:14:10.32 PS.reconfigure PSslow1 @20:14:10.36 PS.seek 1.74ml @20:15:28.25 delay 5 seconds @20:15:33.27 AIV.dial :sample @20:15:33.89 AWV.select :waste @20:15:34.04 PS.reconfigure PSslow2 @20:15:34.09 PS.seek 1.22ml @20:17:21.94 delay 15 seconds @20:17:36.96 AIV.dial :bypass @20:17:37.60 PS.reconfigure PSconfig @20:17:37.65 PS.seek 0ml @20:17:50.88 PSV.dial :air @20:17:51.49 PS.seek 2.5ml @20:18:18.09 PSV.dial :PML @20:18:18.59 PS.seek 0ml @20:18:44.75 AIV.dial :coilToMFB @20:18:44.76 AWV.select :air @20:18:44.92 Extracting Nucleic Acid from AIV @20:18:44.94
Beginning samplePassD @20:18:44.96 SPE.hold 2:46:40,55C @20:18:45.04 ASV.dial :SPEdelivery @20:18:45.12
AWV.select :air @20:18:45.13 AIV.dial :bypass @20:18:45.14 PSV.dial PSR @20:18:45.46 delay 20 seconds @20:18:46.42
Pulling 1.5ml of PSR.killMFB @20:18:46.43 PSR.select :killMFB @20:18:46.55 PS.seek 1.5ml @20:19:03.47 delay 5 seconds @20:19:05.48 primeLysateINT @20:19:05.49 AWV.select :air @20:19:05.51 AIV.dial :coilToMFB @20:19:05.52 ATV.dial AIV @20:19:05.99 AS.reconfigure ASslow1 @20:19:06.04 AS.seek 0.3ml @20:19:08.49
PSV.dial :waste1 @20:19:09.03 PS.seek 0ml @20:19:25.97 PSV.dial PSR @20:19:26.50 PSR.select :air @20:19:26.62 PS.seek 2.5ml @20:19:54.50 PRV.dial CBV @20:19:54.53
CBV.dial PRV @20:19:54.54 PSV.dial :mixloop @20:19:55.74 CTV.dial :air,avoiding: :intake @20:19:55.75 PTV.dial :PRVmixing @20:19:55.77 PS.seek 0ml @20:20:06.71 delay 10 seconds @20:20:16.74 ATV.dial :air2 @20:20:17.66 AS.seek 0.33ml @20:20:23.56
PSV.dial PSR @20:20:23.99 ATV.dial :waste2 @20:20:24.60 AS.reconfigure ASnorm @20:20:24.65 AS.seek :empty @20:20:24.83
Pulling 1.5ml of PSR.flush @20:20:24.84 PSR.select :flush @20:20:24.96 PS.seek 1.5ml @20:20:41.70 Lysate primed! @20:20:41.71 AFV.select :holdingCoil @20:20:41.72 ATV.dial :air2 @20:20:41.88
delay 5 seconds @20:20:42.38 AS.reconfigure ASslow1 @20:20:42.42 AS.seek 0.069ml @20:20:46.90
PSV.dial :waste1 @20:20:47.43 PS.seek 0ml @20:20:56.79 delay 3 seconds @20:20:59.81 ATV.dial :AIV @20:21:00.73 AS.seek 0.469ml @20:21:04.53
PSV.dial PSR @20:21:05.05 PSR.select :air @20:21:05.16 PS.seek 2.5ml @20:21:32.88 PRV.dial CBV @20:21:32.91
CBV.dial PRV @20:21:32.92 PSV.dial :mixloop @20:21:34.11 CTV.dial :air,avoiding: :intake @20:21:34.12 PTV.dial :PRVmixing @20:21:34.14 PS.seek 0ml @20:22:01.60 PSV.dial PSR @20:22:02.95 Pulling 1.5ml of PSR.flush PSR.select :flush @20:22:03.08 PS.seek 1.5ml @20:22:19.66 delay 5 seconds @20:22:21.45 delay 5 seconds @20:22:24.68
PSV.dial :waste1 @20:22:25.21 PS.seek 0ml @20:22:26.52 Solid Phase Extraction Heater at 50.8C @20:22:26.53 ATV.dial ASV @20:22:26.93 ASV.dial :SPEdelivery @20:22:27.00 AS.seek 0.041ml @20:22:41.69
PSV.dial PSR @20:22:42.20 PSR.select :air @20:22:42.31 PS.seek 2.5ml @20:23:09.28 PRV.dial CBV @20:23:09.75
CBV.dial PRV @20:23:09.77 PSV.dial :mixloop @20:23:10.92 CTV.dial :air,avoiding: :intake @20:23:10.93 PTV.dial :PRVmixing @20:23:10.95 PS.seek 0ml @20:23:37.85 PSV.dial PSR @20:23:39.06 Pulling 1.5ml of PSR.flush PSR.select :flush @20:23:39.19 PS.seek 1.5ml @20:23:53.38 delay 10 seconds @20:23:55.66
delay 5 seconds @20:24:00.68 PSV.dial :waste1 @20:24:01.18 PS.seek 0ml @20:24:03.41 rinseLysateLineINT AFV.select :carrierSolution @20:24:03.52 delay 2 seconds @20:24:05.54 AS.reconfigure ASconfig @20:24:05.59 AS.seek 1ml @20:24:17.60
PSV.dial PSR @20:24:18.11 PSR.select :air @20:24:18.23 PS.seek 2.5ml @20:24:44.94 PRV.dial CBV @20:24:44.97
CBV.dial PRV @20:24:44.98 PSV.dial :mixloop @20:24:46.11 CTV.dial :air,avoiding: :intake @20:24:46.12 PTV.dial :PRVmixing @20:24:46.14 PS.reconfigure PSslow @20:24:46.18 PS.seek 1.2ml @20:25:00.98 delay 1 second @20:25:02.00 AFV.select :holdingCoil @20:25:02.11 delay 1 second @20:25:03.12 ATV.dial AIV @20:25:03.68 AIV.dial :coilToMFB @20:25:03.69 AWV.select :waste @20:25:03.80 AS.reconfigure ASslow0 @20:25:03.85 AS.seek 0ml @20:25:12.97
PSV.dial :waste1 @20:25:14.47 PS.reconfigure PSconfig @20:25:14.51 PS.seek 0ml @20:25:27.53 PSV.dial :air @20:25:28.81 PS.seek 2.5ml @20:25:55.38 PSV.dial :mixloop @20:25:55.89 PS.reconfigure PSslow @20:25:55.93 PS.seek 0ml @20:26:44.76 ATV.dial :air2 @20:26:45.44 AS.reconfigure ASslow1 @20:26:45.48 AS.seek 0.05ml @20:26:47.07
PSV.dial :air @20:26:47.58 PS.reconfigure PSconfig @20:26:47.62 PS.seek 2.5ml @20:26:55.82 ATV.dial :AIV @20:26:56.66 AS.seek 0.01ml @20:27:05.01 ATV.dial :waste @20:27:06.70 AS.seek :empty @20:27:09.13 ethanol wash @20:27:09.14 ATV.dial :air2 @20:27:10.41 AS.reconfigure ASslow2 @20:27:10.45 AS.seek 0.009ml @20:27:13.94
PSV.dial :mixloop @20:27:14.45 PS.reconfigure PSslow @20:27:14.49 PS.seek 0ml @20:27:21.04 delay 3 seconds @20:27:24.07 Pulling 0.4ml of Ethanol Wash ATV.dial :ethanol @20:27:24.91 AS.reconfigure ASslow1 @20:27:24.95 AS.seek 0.409ml @20:28:05.63
Cleaning Collection Syringe @20:28:05.64 CSV.dial CSR @20:28:06.19 Pulling 4ml of CSR.flush CSR.select :flush @20:28:06.31 CS.seek 4ml @20:28:17.73 delay 5 seconds @20:28:22.75 CSR.select :air @20:28:22.90 CS.seek 10ml @20:28:39.48 CSV.dial CTV @20:28:39.50
CTV.dial CSV @20:28:41.15 CBV.dial :waste1 @20:28:41.71 CS.seek 0ml @20:28:45.68 delay 3 seconds @20:28:48.70 ATV.dial :air1 @20:28:49.10 delay 3 seconds @20:28:52.63 AS.seek 0.434ml @20:28:57.92 delay 3 seconds @20:29:00.94 ATV.dial :ethanol @20:29:01.57 AS.seek 0.419ml @20:29:04.87 delay 3 seconds @20:29:07.89 ATV.dial ASV @20:29:08.73
CSV.dial CSR @20:29:09.30 AS.seek 0.092ml @20:29:09.39
Pulling 4ml of CSR.flush @20:29:09.40 CSR.select :flush @20:29:09.52 CS.seek 4ml @20:29:20.85 delay 5 seconds @20:29:25.87 CSR.select :air @20:29:25.99 CS.seek 10ml @20:29:42.57 CSV.dial CTV @20:29:42.60
CTV.dial CSV @20:29:43.14 CBV.dial :waste1 @20:29:43.16 CS.seek 0ml @20:30:10.29 Garaging FlushPuck in Collection Clamp @20:30:10.35 Elbow.reconfigure TightElbow @20:30:10.40 Elbow.seek CC @20:30:10.63 Elbow.reconfigure LooseElbow @20:30:10.72 Forearm.seek CC @20:30:11.77 Hand.close @20:30:12.50 CC.seek :open @20:30:15.29 delay 3 seconds @20:30:16.87
Forearm.seek :retract @20:30:17.97 Elbow.reconfigure TightElbow @20:30:18.01 Elbow.seek :garage @20:30:18.31 ATV.dial :waste2 @20:30:19.07 AS.reconfigure ASconfig @20:30:19.12 AS.seek :empty @20:30:21.26
Elbow.reconfigure LooseElbow @20:30:21.34 Forearm.reconfigure TightForearm @20:30:21.39 Forearm.seek :garage @20:30:22.52 Hand.open @20:30:23.30 Forearm.reconfigure LooseForearm @20:30:23.34 Forearm.seek :retract @20:30:24.40 Whole Cell Archive sampling 100ml @20:30:24.42 Loading wcr puck from tube 2 into Collection Clamp @20:30:24.64 SC.reconfigure SCtight @20:30:24.68 SC.seek 2 @20:30:24.77 ATV.dial :air2 @20:30:25.19 AS.reconfigure ASslow1 @20:30:25.23 AS.seek 1ml @20:30:27.06 SC.reconfigure SCloose @20:30:27.26 Elbow.reconfigure TightElbow @20:30:27.30 Elbow.seek 2 @20:30:29.47 Forearm.reconfigure TightForearm @20:30:29.52 Forearm.seek 2 + 700 counts @20:30:30.55 SE.reconfigure SEpick @20:30:30.62 SE.seek beamBreak @20:30:33.67 SE.configure SEconfig @20:30:33.81 Elbow.seek 2 @20:30:33.99 Forearm.seek 2 + 300 counts @20:30:34.22 Forearm.reconfigure LooseForearm @20:30:34.26 Hand.close @20:30:35.47 SE.seek :down @20:30:35.67 Forearm.seek :retract @20:30:36.66 Elbow.seek CC @20:30:38.28 Elbow.reconfigure LooseElbow @20:30:38.38 SE.coast @20:30:38.44 Forearm.reconfigure TightForearm @20:30:38.48 Forearm.seek CC @20:30:39.82 CC.seek :closed @20:30:44.06 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.40 @20:30:44.07 Hand.open @20:30:44.94 Forearm.reconfigure LooseForearm @20:30:44.99 Forearm.seek :retract @20:30:45.99 SC.coast @20:30:46.03 collectSample 100ml w/bubble point of 55psi @20:30:46.04 Priming 7ml @20:30:46.05 Gate.power :sampler,:ON @20:30:46.06 Intake.select :close @20:30:46.07 Exhaust.select :close @20:30:46.08 SS.hold @20:30:46.27 Calibrating pressure sensors @20:30:46.28 CTV.dial :air @20:30:47.84 SSV.dial :spit,avoiding: :exhaust @20:30:47.86 SS.reconfigure SSfast @20:30:47.91 SS.seek 5ml @20:30:50.69 SSV.dial CBV @20:30:50.72 CBV.dial SSV @20:30:52.36 delay 3.8 seconds @20:30:56.29 SSV.dial :spit @20:30:57.82 SS.seek 0ml @20:31:00.84 SSV.dial CBV @20:31:02.36 CTV.dial :intake @20:31:03.21 Waiting for external sampler to finish priming... @20:31:03.22 delayUntil "20:35:46.06" @20:33:46.81 delay 5 seconds @20:33:51.83 ATV.dial ASV @20:33:52.85 ASV.dial :SPEdelivery @20:33:52.87 AS.seek 0.02ml @20:35:46.08 SSV.dial CBV @20:35:46.10 CBV.dial SSV @20:35:46.12 Allowing 40s for 7.0ml of remaining 7.0ml @20:35:46.13 Intake.select :open @20:35:48.28 Intake @15.9psia, vacuum from 9.4 to 13.4psi @20:35:48.30 SS.reconfigure SSinhale.with maxSpeed: 150 @20:35:48.34 SS.seek 7ml @20:35:54.56 Stroke completed -- waiting for pressure to equalize ... @20:36:12.18 Intake.select :close @20:36:12.33 SSV.dial :exhaust @20:36:13.19 Exhaust.select :open @20:36:13.30 SS.reconfigure SSexhale @20:36:13.35 SS.seek 0ml @20:36:17.53 Exhaust.select :close @20:36:17.64 Sampling 100.0ml @20:36:17.67 Intake.select :close @20:36:17.68 Exhaust.select :close @20:36:17.69 SS.reconfigure SSconfig @20:36:17.73 SS.hold @20:36:17.93 CTV.dial :intake @20:36:17.94 SSV.dial CBV @20:36:17.97 CBV.dial SSV @20:36:18.86 Allowing 130s for 25.0ml of remaining 100.0ml @20:36:18.87 Intake.select :open @20:36:21.03 Intake @15.9psia, vacuum from 9.4 to 13.4psi @20:36:21.05 SS.reconfigure SSinhale.with maxSpeed: 150 @20:36:21.09 SS.seek 25ml @20:36:25.37 Vacuum too high -- waiting up to 40s @20:36:33.76 Intake @16.1psia, vacuum from 9.6 to 13.6psi @20:36:33.77 SS.reconfigure SSinhale.with maxSpeed: 135 @20:36:33.82 SS.seek 25ml @20:36:37.68 Vacuum too high -- waiting up to 40s @20:36:46.28 SS.reconfigure SSinhale.with maxSpeed: 122 @20:36:46.33 SS.seek 25ml @20:36:50.43 Vacuum too high -- waiting up to 40s @20:36:59.76 Intake @16.2psia, vacuum from 9.7 to 13.7psi @20:36:59.78 SS.reconfigure SSinhale.with maxSpeed: 110 @20:36:59.82 SS.seek 25ml @20:37:04.21 Vacuum too high -- waiting up to 40s @20:37:10.25 delay 5 seconds @20:37:13.14 Intake @16.1psia, vacuum from 9.6 to 13.6psi @20:37:13.16 SS.reconfigure SSinhale.with maxSpeed: 99 @20:37:13.20 SS.seek 25ml @20:37:15.27 ATV.dial :air2 @20:37:16.37 AS.seek 0.52ml @20:37:17.17 Stroke completed -- waiting for pressure to equalize ... @20:37:27.62 Intake.select :close @20:37:27.77 SSV.dial :exhaust @20:37:28.64 Exhaust.select :open @20:37:28.75 SS.reconfigure SSexhale @20:37:28.80 SS.seek 0ml @20:37:38.42 Exhaust.select :close @20:37:38.53 SSV.dial CBV @20:37:38.55 CBV.dial SSV @20:37:39.42 Allowing 130s for 25.0ml of remaining 75.0ml @20:37:39.43 Intake.select :open @20:37:41.59 Intake @15.9psia, vacuum from 9.4 to 13.4psi @20:37:41.61 SS.reconfigure SSinhale.with maxSpeed: 99 @20:37:41.65 SS.seek 25ml @20:37:46.06 Vacuum too high -- waiting up to 40s @20:37:52.60 Intake @16.0psia, vacuum from 9.5 to 13.5psi @20:37:52.62 SS.reconfigure SSinhale.with maxSpeed: 89 @20:37:52.66 SS.seek 25ml @20:37:56.93 Vacuum too high -- waiting up to 40s @20:38:03.92 SS.reconfigure SSinhale.with maxSpeed: 80 @20:38:03.96 SS.seek 25ml @20:38:08.49 Vacuum too high -- waiting up to 40s @20:38:16.24 SS.reconfigure SSinhale.with maxSpeed: 72 @20:38:16.29 SS.seek 25ml @20:38:21.24 Vacuum too high -- waiting up to 40s @20:38:29.61 Intake @15.9psia, vacuum from 9.4 to 13.4psi @20:38:29.63 SS.reconfigure SSinhale.with maxSpeed: 65 @20:38:29.67 SS.seek 25ml @20:38:35.37 Vacuum too high -- waiting up to 40s @20:38:44.23 Intake @16.0psia, vacuum from 9.5 to 13.5psi @20:38:44.25 SS.reconfigure SSinhale.with maxSpeed: 59 @20:38:44.29 SS.seek 25ml @20:38:48.18 Stroke completed -- waiting for pressure to equalize ... @20:38:57.24 delay 5 seconds @20:38:57.41 Intake.select :close @20:38:57.56 SSV.dial :exhaust @20:38:58.42 Exhaust.select :open @20:38:58.53 SS.reconfigure SSexhale @20:38:58.58 SS.seek 0ml @20:39:02.26 ATV.dial ASV @20:39:03.27 ASV.dial :wasteR @20:39:03.65 AS.seek 0.3ml @20:39:08.11 Exhaust.select :close @20:39:08.22 SSV.dial CBV @20:39:08.71 CBV.dial SSV @20:39:09.62 Allowing 130s for 25.0ml of remaining 50.0ml @20:39:09.63 Intake.select :open @20:39:11.80 Intake @15.9psia, vacuum from 9.4 to 13.4psi @20:39:11.82 SS.reconfigure SSinhale.with maxSpeed: 59 @20:39:11.86 SS.seek 25ml @20:39:17.56 Vacuum too high -- waiting up to 40s @20:39:23.41 Intake @15.8psia, vacuum from 9.3 to 13.3psi @20:39:23.43 SS.reconfigure SSinhale.with maxSpeed: 53 @20:39:23.47 SS.seek 25ml @20:39:29.34 Vacuum too high -- waiting up to 40s @20:39:36.82 Intake @15.9psia, vacuum from 9.4 to 13.4psi @20:39:36.83 SS.reconfigure SSinhale.with maxSpeed: 48 @20:39:36.88 SS.seek 25ml @20:39:44.24 Vacuum too high -- waiting up to 40s @20:39:48.19 delay 20 seconds @20:39:51.70 Intake @15.9psia, vacuum from 9.4 to 13.4psi @20:39:51.72 SS.reconfigure SSinhale.with maxSpeed: 43 @20:39:51.76 SS.seek 25ml @20:40:00.62 Vacuum too high -- waiting up to 40s @20:40:08.27 AS.seek 0.07ml @20:40:08.84 Intake @15.8psia, vacuum from 9.3 to 13.3psi @20:40:08.86 SS.reconfigure SSinhale.with maxSpeed: 39 @20:40:08.90 SS.seek 25ml @20:40:21.76 Stroke completed -- waiting for pressure to equalize ... @20:40:35.25 Intake.select :close @20:40:35.40 SSV.dial :exhaust @20:40:36.26 Exhaust.select :open @20:40:36.37 SS.reconfigure SSexhale @20:40:36.42 SS.seek 0ml @20:40:45.92 Exhaust.select :close @20:40:46.03 SSV.dial CBV @20:40:46.07 CBV.dial SSV @20:40:46.90 Allowing 130s for 25.0ml of remaining 25.0ml @20:40:46.91 Intake.select :open @20:40:49.07 Intake @15.8psia, vacuum from 9.3 to 13.3psi @20:40:49.09 SS.reconfigure SSinhale.with maxSpeed: 39 @20:40:49.13 SS.seek 25ml @20:40:54.77 delay 20 seconds @20:40:59.59 Vacuum too high -- waiting up to 40s @20:41:05.91 Intake @15.7psia, vacuum from 9.2 to 13.2psi @20:41:05.93 SS.reconfigure SSinhale.with maxSpeed: 35 @20:41:05.97 SS.seek 25ml @20:41:14.79 ATV.dial :waste2 @20:41:15.57 AS.reconfigure ASconfig @20:41:15.61 AS.seek :empty @20:41:17.43 Vacuum too high -- waiting up to 40s @20:41:20.00 Elution @20:41:20.02 ATV.dial :air1 @20:41:21.25 AS.reconfigure ASslow1 @20:41:21.29 AS.seek 0.049ml @20:41:25.67 Intake @15.8psia, vacuum from 9.3 to 13.3psi @20:41:25.68 SS.reconfigure SSinhale.with maxSpeed: 32 @20:41:25.73 SS.seek 25ml @20:41:31.59 delay 3 seconds @20:41:34.61 ATV.dial :ElutionWater @20:41:35.33 AS.seek 0.109ml @20:41:44.06 Vacuum too high -- waiting up to 40s @20:41:47.65 delay 3 seconds @20:41:50.67 ATV.dial ASV,via: :air2 @20:41:52.01 Intake @15.7psia, vacuum from 9.2 to 13.2psi @20:41:52.03 SS.reconfigure SSinhale.with maxSpeed: 29 @20:41:52.07 SS.seek 25ml @20:41:52.90 ASV.dial :SPEelution @20:41:53.49 AFV.select :carrierSolution @20:41:53.64 AS.reconfigure ASnorm @20:41:53.69 AS.seek 0.759ml @20:42:06.50 Stroke completed -- waiting for pressure to equalize ... @20:42:16.84 Intake.select :close @20:42:16.99 SSV.dial :exhaust @20:42:17.86 Exhaust.select :open @20:42:17.97 SS.reconfigure SSexhale @20:42:18.02 SS.seek 0ml @20:42:26.74 delay 3 seconds @20:42:27.71 Exhaust.select :close @20:42:27.82 Sampled 100.0ml @20:42:27.85 Drying puck before fixing for 4:00 @20:42:27.86 CBV.dial :waste2b @20:42:28.74 delay 1:00 @20:42:29.76 AFV.select :holdingCoil @20:42:29.91 AS.reconfigure ASslow1 @20:42:29.96 AS.seek 0.474ml @20:42:30.82 Gate.power :sampler,:OFF @20:43:27.55 delay 5 seconds @20:43:28.76 CSV.dial :air @20:43:29.28 CS.reconfigure CSfast @20:43:29.32 CS.seek 10ml @20:43:32.63 AS.reconfigure ASslow2 @20:43:32.68 AS.seek 0.439ml @20:43:56.05 Pumping wcr puck from tube 2 up to 55psi... @20:43:56.07 CBV.dial SSV @20:43:57.50 CBV.dial between CSV and waste2b @20:43:58.61 CSV.dial between air and CTV @20:43:58.98 CS.seek 7.253ml @20:44:07.08 CTV.dial CSV,avoiding: :air @20:44:07.11 CSV.dial CTV @20:44:08.00 delay 1:00 @20:44:08.03 CS.reconfigure CSpressurize @20:44:08.08 CS.seek 0ml @20:44:27.29 CBV.dial :waste2b @20:44:27.66 CS.reconfigure CSconfig @20:44:27.71 CS.seek :empty @20:44:28.52 delay 1:00 @20:45:08.06 pushing eluate to holding coil @20:45:08.12 AS.seek 0.279ml @20:45:28.54 CTV.dial :air,avoiding: :intake @20:45:30.09 Fix #1 of 2 (with rnal) for 2:00 @20:45:30.10 CSV.dial CSR @20:45:30.66 Pulling 2ml of CSR.rnal @20:45:30.67 CSR.select :rnal @20:45:30.78 CS.seek 2ml @20:45:37.05 delay 5 seconds @20:45:42.07 CSR.select :air @20:45:42.19 CS.reconfigure CSfast @20:45:42.24 CS.seek 10ml @20:46:03.71 Pumping wcr puck from tube 2 up to 55psi... @20:46:03.73 CBV.dial SSV @20:46:05.14 CBV.dial between CSV and waste2b @20:46:06.25 CSV.dial between air and CTV @20:46:07.08 CS.seek 7.266ml @20:46:15.18 CTV.dial CSV,avoiding: :air @20:46:15.21 CSV.dial CTV @20:46:16.81 CS.reconfigure CSpressurize @20:46:16.85 CS.seek 0ml @20:46:35.07 delay 2:00 @20:47:49.15 delay 30 seconds @20:48:19.18 ATV.dial :waste,via: :air3 @20:48:21.42 AS.reconfigure ASconfig @20:48:21.49 AS.seek :empty @20:48:35.09 CBV.dial :waste2b @20:48:35.46 CS.reconfigure CSconfig @20:48:35.51 CS.seek :empty @20:48:37.91 Parking Bleach in LysateSource line and SPEcolumn @20:48:37.93 delay 1:00 @20:48:37.95 ATV.dial :air3 @20:48:38.82 AS.reconfigure ASslow1 @20:48:38.86 AS.seek 0.029ml @20:48:45.26 ATV.dial :ElutionWater @20:48:45.86 AS.seek 0.059ml @20:48:52.20 ATV.dial :air3 @20:48:52.80 AS.seek 0.069ml @20:48:55.07 delay 2 seconds @20:48:57.09 ATV.dial :bleach @20:48:57.55 AS.seek 0.769ml @20:49:37.96 CTV.dial :air,avoiding: :intake @20:49:39.52 Fix #2 of 2 (with rnal) for 2:00 @20:49:39.53 CSV.dial CSR @20:49:40.08 Pulling 2ml of CSR.rnal @20:49:40.09 CSR.select :rnal @20:49:40.20 CS.seek 2ml @20:49:46.43 delay 5 seconds @20:49:51.45 CSR.select :air @20:49:51.57 CS.reconfigure CSfast @20:49:51.62 CS.seek 10ml @20:50:13.24 Pumping wcr puck from tube 2 up to 55psi... @20:50:13.25 CBV.dial SSV @20:50:14.69 CBV.dial between CSV and waste2b @20:50:15.82 CSV.dial between air and CTV @20:50:16.53 CS.seek 7.253ml @20:50:24.77 CTV.dial CSV,avoiding: :air @20:50:24.80 CSV.dial CTV @20:50:26.42 CS.reconfigure CSpressurize @20:50:26.46 CS.seek 0ml @20:50:44.61 delay 2:00 @20:51:18.57 delay 3 seconds @20:51:21.59 ATV.dial :air3 @20:51:22.15 delay 3 seconds @20:51:25.22 AS.seek 0.794ml @20:51:30.52 delay 3 seconds @20:51:33.54 ATV.dial :bleach @20:51:34.16 AS.seek 0.779ml @20:51:37.48 delay 3 seconds @20:51:40.50 ATV.dial :AIV @20:51:41.79 AS.reconfigure ASconfig @20:51:41.84 AS.seek 0.015ml @20:52:26.04 ATV.dial :waste @20:52:27.57 AS.seek :empty @20:52:28.77 ATV.dial :air3 @20:52:29.46 AS.reconfigure ASslow1 @20:52:29.50 AS.seek 0.009ml @20:52:31.95 delay 2 seconds @20:52:33.97 ATV.dial :bleach @20:52:34.43 AS.seek 0.579ml @20:52:44.63 CBV.dial :waste2b @20:52:44.99 CS.reconfigure CSconfig @20:52:45.04 CS.seek :empty @20:52:47.37 delay 1:00 @20:53:47.40 CTV.dial :air,avoiding: :intake @20:53:49.01 Storing wcr puck in Collection Clamp to tube 1 @20:53:49.17 SC.reconfigure SCtight @20:53:49.21 SC.seek 1 @20:53:51.37 SC.reconfigure SCloose @20:53:52.04 Elbow.reconfigure TightElbow @20:53:52.08 Elbow.seek 1 @20:53:53.98 Forearm.reconfigure TightForearm @20:53:54.03 Forearm.seek 1 + 700 counts @20:53:55.05 SE.reconfigure SEpick @20:53:55.12 SE.seek beamBreak @20:53:58.26 SE.configure SEconfig @20:53:58.41 Elbow.reconfigure LimpElbow @20:53:58.46 Forearm.reconfigure LooseForearm @20:53:58.50 Forearm.seek 1 @20:53:58.83 Elbow.hold @20:53:58.91 Elbow.reconfigure TightElbow @20:53:59.00 SE.seek 1.07 Pucks below Top Plate @20:53:59.49 Forearm.force -20 @20:53:59.53 Forearm.seek 1 + 322 counts @20:53:59.84 Forearm.reconfigure TightForearm @20:53:59.88 Forearm.seek 1 + 700 counts @20:54:00.20 SE.seek 2.07 Pucks below Top Plate @20:54:00.89 Forearm.reconfigure LooseForearm @20:54:00.94 Forearm.seek :retract @20:54:01.86 Elbow.seek CC @20:54:03.53 Elbow.reconfigure LooseElbow @20:54:03.62 Forearm.seek CC @20:54:04.67 Hand.close @20:54:05.40 CC.seek :open @20:54:09.85 Forearm.seek :retract @20:54:10.91 Elbow.reconfigure TightElbow @20:54:10.96 Elbow.seek 1 @20:54:12.94 Elbow.reconfigure LimpElbow @20:54:12.99 Forearm.seek 1 @20:54:14.05 Elbow.hold @20:54:14.12 Elbow.reconfigure TightElbow @20:54:14.16 Hand.open @20:54:15.01 Forearm.force -20 @20:54:15.05 Forearm.seek 1 + 347 counts @20:54:15.36 Forearm.reconfigure TightForearm @20:54:15.40 Forearm.seek 1 + 700 counts @20:54:15.77 SE.seek :down @20:54:16.91 Forearm.reconfigure LooseForearm @20:54:16.95 Forearm.seek :retract @20:54:18.43 SE.coast @20:54:18.46 SC.coast @20:54:18.97 Starting finalPurge @20:54:18.98 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:54:19.05 Hand.open @20:54:19.39 Elbow.seek FlushPuck::Garage @20:54:21.48 Elbow.reconfigure LooseElbow @20:54:21.57 Forearm.seek :garage @20:54:22.61 Hand.close @20:54:23.51 Forearm.seek :retract @20:54:24.56 Elbow.reconfigure TightElbow @20:54:24.61 Elbow.seek CC @20:54:27.56 Elbow.reconfigure LooseElbow @20:54:27.65 Forearm.reconfigure TightForearm @20:54:27.69 Forearm.seek CC @20:54:29.01 CC.seek :closed @20:54:29.34 delay 3 seconds @20:54:32.38 ATV.dial :air3 @20:54:32.96 delay 3 seconds @20:54:33.26
Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.42 @20:54:33.33 Hand.open @20:54:34.08 Forearm.reconfigure LooseForearm @20:54:34.12 Forearm.seek :retract @20:54:35.16 Flushing the Collection Valve::Manifold @20:54:35.28 CSV.dial CSR @20:54:35.85 Pulling 5ml of CSR.flush CSR.select :flush @20:54:35.97 CS.seek 5ml @20:54:36.06 AS.seek 0.604ml @20:54:41.37 delay 3 seconds @20:54:44.39 ATV.dial :bleach @20:54:45.01 AS.seek 0.589ml @20:54:48.30 delay 3 seconds @20:54:50.06
delay 5 seconds @20:54:51.32 ATV.dial :ASV @20:54:52.38 ASV.dial :wasteR @20:54:53.06 AS.seek 0ml @20:54:55.08
CSR.select :air @20:54:55.23 CS.reconfigure CSfast @20:54:55.27 CS.seek 7.5ml @20:55:02.68 CSV.dial :waste @20:55:04.21 CS.reconfigure CSconfig @20:55:04.26 CS.seek 0ml @20:55:24.62 Flushing lower manifold's waste1 port @20:55:24.74 CSV.dial :flush @20:55:25.25 CS.reconfigure CSfast @20:55:25.29 CS.seek 5ml @20:55:39.12 delay 5 seconds @20:55:44.14 CSV.dial :air @20:55:44.73 CS.seek 7ml @20:55:50.87 CSV.dial CTV @20:55:50.90
CTV.dial CSV @20:55:52.49 CBV.dial :waste1 @20:55:53.38 CS.seek 0ml @20:56:12.28 Flushing lower manifold's waste2b port @20:56:12.40 CSV.dial :flush @20:56:13.78 CS.seek 5ml @20:56:27.68 delay 5 seconds @20:56:32.70 CSV.dial :air @20:56:33.31 CS.seek 7ml @20:56:39.49 CSV.dial CTV @20:56:39.52
CTV.dial CSV @20:56:40.03 CBV.dial :waste2b @20:56:40.91 CS.seek 0ml @20:56:51.79 delay 1:00 @20:56:59.93
Flushing lower manifold's waste1 port @20:56:59.94 CSV.dial CSR @20:57:00.52 Pulling 4ml of CSR.mfbkill @20:57:00.53 CSR.select :mfbkill @20:57:00.64 CS.reconfigure CSconfig @20:57:00.69 CS.seek 4ml @20:57:11.99 delay 5 seconds @20:57:17.01 CSR.select :air @20:57:17.13 CS.reconfigure CSfast @20:57:17.17 CS.seek 10ml @20:57:33.68 CSV.dial CTV @20:57:33.71
CTV.dial CSV @20:57:34.25 CBV.dial :waste1 @20:57:35.15 CS.seek 0ml @20:57:51.81 AFV.select :carrierSolution @20:57:51.92 delay 2 seconds @20:57:53.94 AS.reconfigure ASconfig @20:57:53.99 AS.seek 1ml @20:58:01.90
Flushing lower manifold's waste2b port @20:58:01.91 CSV.dial CSR @20:58:02.49 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @20:58:02.60 CS.reconfigure CSconfig @20:58:02.65 CS.seek 4ml @20:58:13.90 delay 5 seconds @20:58:18.92 CSR.select :air @20:58:19.04 CS.reconfigure CSfast @20:58:19.08 CS.seek 10ml @20:58:35.53 CSV.dial CTV @20:58:35.56
CTV.dial CSV @20:58:36.10 CBV.dial :waste2b @20:58:36.99 CS.seek 0ml @20:58:51.73 delay 1 second @20:58:52.75 AFV.select :holdingCoil @20:58:52.86 delay 1 second @20:58:53.87 ATV.dial ASV @20:58:53.88 ASV.dial :wasteR @20:58:53.90 AS.reconfigure ASslow1 @20:58:53.95 AS.seek 0ml @20:59:03.68
Killing upper manifold @20:59:03.69 CSV.dial CSR @20:59:04.26 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @20:59:04.37 CS.reconfigure CSconfig @20:59:04.42 CS.seek 4ml @20:59:15.75 delay 5 seconds @20:59:20.77 CSR.select :air @20:59:20.89 CS.reconfigure CSfast @20:59:20.94 CS.seek 10ml @20:59:37.28 CSV.dial CBV @20:59:37.32
CBV.dial CSV @20:59:38.56 CTV.dial :air @20:59:40.12 CS.seek 0ml @21:00:06.84 delay 7 seconds @21:00:13.87 CS.seek 3ml @21:00:22.65 CSV.dial :waste @21:00:23.16 CS.seek 0ml @21:00:31.81 Flushing upper manifold @21:00:31.93 CSV.dial :flush @21:00:32.46 CS.seek 5ml @21:00:46.28 delay 5 seconds @21:00:51.30 CSV.dial :air @21:00:51.90 CS.seek 7ml @21:00:58.12 CSV.dial CBV @21:00:58.16
CBV.dial CSV @21:00:59.37 CTV.dial :air @21:00:59.39 CS.seek 0ml @21:01:18.72 delay 7 seconds @21:01:25.75 CS.seek 3ml @21:01:34.40 CSV.dial :waste @21:01:34.95 CS.seek 0ml @21:01:44.13 CSV.dial :flush @21:01:44.66 CS.seek 3ml @21:01:53.34 delay 5 seconds @21:01:58.36 CSV.dial :air @21:01:58.94 CS.seek 5ml @21:02:05.12 CSV.dial :air @21:02:05.14 CS.seek 0ml @21:02:15.32 delay 20 seconds @21:02:19.06
delay 7 seconds @21:02:26.09 CS.seek 3ml @21:02:34.75 CSV.dial :waste @21:02:35.34 ATV.dial :air3 @21:02:35.63
CS.seek 0ml @21:02:36.90 AS.seek 0.59ml @21:02:44.34
Flushing lower manifold's waste1 port @21:02:44.46 CSV.dial :flush @21:02:44.99 CS.seek 5ml @21:02:58.97 delay 5 seconds @21:03:03.99 CSV.dial :air @21:03:04.56 CS.seek 7ml @21:03:10.75 CSV.dial CTV @21:03:10.79
CTV.dial CSV @21:03:12.39 CBV.dial :waste1 @21:03:12.93 CS.seek 0ml @21:03:31.94 Flushing lower manifold's waste2b port @21:03:32.06 CSV.dial :flush @21:03:32.95 CS.seek 5ml @21:03:46.81 delay 5 seconds @21:03:51.83 CSV.dial :air @21:03:52.43 CS.seek 7ml @21:03:58.69 CSV.dial CTV @21:03:58.73
CTV.dial CSV @21:03:59.22 CBV.dial :waste2b @21:04:00.12 CS.seek 0ml @21:04:19.12 Garaging FlushPuck in Collection Clamp @21:04:19.19 Elbow.reconfigure TightElbow @21:04:19.23 Elbow.seek CC @21:04:19.48 Elbow.reconfigure LooseElbow @21:04:19.57 Forearm.seek CC @21:04:20.61 Hand.close @21:04:21.41 CC.seek :open @21:04:25.77 Forearm.seek :retract @21:04:26.84 Elbow.reconfigure TightElbow @21:04:26.88 Elbow.seek :garage @21:04:30.10 Elbow.reconfigure LooseElbow @21:04:30.18 Forearm.reconfigure TightForearm @21:04:30.22 Forearm.seek :garage @21:04:31.39 Hand.open @21:04:32.21 Forearm.reconfigure LooseForearm @21:04:32.25 Forearm.seek :retract @21:04:33.26 Completed [mbaPhase] begun 16:56:56 @21:04:33.27 Extending 190515totalsampletest until completion of MFBPCR @21:04:35.85 delay 3 seconds @21:04:38.87 ATV.dial :ASV @21:04:40.32 ASV.dial :wasteR @21:04:40.39 AS.seek 0.09ml @21:06:21.21 delay 30 seconds @21:06:51.24 ATV.dial :extSrc @21:06:52.02 delay 3 seconds @21:06:55.04 ATV.dial :ElutionWater @21:06:56.19 AS.seek 0.12ml @21:07:02.55 ATV.dial :air3 @21:07:03.18 AS.seek 0.13ml @21:07:05.51 delay 2 seconds @21:07:07.53 ATV.dial :bleach @21:07:08.46 AS.seek 0.23ml @21:07:28.91 delay 3 seconds @21:07:31.93 ATV.dial :air3 @21:07:32.49 delay 3 seconds @21:07:35.56 AS.seek 0.255ml @21:07:40.89 delay 3 seconds @21:07:43.91 ATV.dial :bleach @21:07:44.54 AS.seek 0.24ml @21:07:47.84 delay 3 seconds @21:07:50.86 ATV.dial :ASV @21:07:51.94 ASV.dial :SPEdelivery @21:07:52.32 AFV.select :carrierSolution @21:07:52.43 delay 2 seconds @21:07:54.45 AS.reconfigure ASconfig @21:07:54.50 AS.seek 0.5ml @21:08:09.71 delay 1 second @21:08:10.73 AFV.select :holdingCoil @21:08:10.88 AS.reconfigure ASslow1 @21:08:10.93 AS.seek 0.17ml @21:09:17.60 SPE.hold 2:46:40,95C @21:09:17.63 delay 3:00 @21:12:17.66 AFV.select :carrierSolution @21:12:17.77 delay 2 seconds @21:12:19.79 AS.reconfigure ASconfig @21:12:19.84 AS.seek 1ml @21:13:07.84 delay 1 second @21:13:08.86 AFV.select :holdingCoil @21:13:08.97 delay 1 second @21:13:09.98 ASV.dial :wasteD @21:13:11.06 AS.seek 0ml @21:14:08.82 Priming Eluate @21:14:08.83 AFV.select :carrierSolution @21:14:08.94 delay 2 seconds @21:14:10.96 AS.seek 0.5ml @21:14:39.99 delay 1 second @21:14:41.01 AFV.select :holdingCoil @21:14:41.11 ATV.dial ASV @21:14:41.13 ASV.dial :SPErecovery @21:14:41.73 Swish #1 of 2 @21:14:41.80 AS.reconfigure ASslow0 @21:14:41.84 AS.seek 0.6ml @21:14:52.30 AS.seek 0.5ml @21:15:02.66 Swish #2 of 2 @21:15:02.73 AS.seek 0.6ml @21:15:13.24 AS.seek 0.5ml @21:15:23.63 delay 3 seconds @21:15:26.70 AS.reconfigure ASslow1 @21:15:26.75 AS.seek 0.684ml @21:16:04.04 delay 3 seconds @21:16:07.06 Eluate primed @21:16:07.07 ATV.dial :waste2 @21:16:07.70 AS.reconfigure ASnorm @21:16:07.74 AS.seek 0ml @21:16:42.54 SPE.coast @21:16:42.61 ASV.dial :SPErecovery @21:16:42.62 Setting up PCR for IPc -- writing /var/log/esp/IPc19may1521h100ml.pcr @21:16:42.64 Building PCR Slug from ASV with MM1 mix, PP1 primer @21:16:42.65 Priming with carrier @21:16:42.66 ATV.dial ARV @21:16:43.65 ARV.dial :carrier @21:16:43.99 AS.seek 0.5ml @21:17:09.54 AFV.select :carrierSolution @21:17:09.65 delay 2 seconds @21:17:11.67 AS.reconfigure ASconfig @21:17:11.72 AS.seek 1ml @21:17:40.73 delay 1 second @21:17:41.75 AFV.select :holdingCoil @21:17:41.86 delay 1 second @21:17:42.87 ATV.dial :waste @21:17:44.42 AS.reconfigure ASnorm @21:17:44.46 AS.seek :empty @21:18:35.19 AFV.select :carrierSolution @21:18:35.30 delay 2 seconds @21:18:37.32 AS.reconfigure ASconfig @21:18:37.37 AS.seek 0.5ml @21:19:06.48 delay 1 second @21:19:07.50 AFV.select :holdingCoil @21:19:07.61 Building Slug @21:19:07.62 ATV.dial :ARV @21:19:09.29 delay 2 seconds @21:19:11.31 ARV.dial :air1 @21:19:11.70 delay 2 seconds @21:19:13.77 AS.reconfigure ASslow2 @21:19:13.82 AS.seek 0.506ml @21:19:20.32 delay 2 seconds @21:19:22.34 ARV.dial :MM1 @21:19:22.93 delay 2 seconds @21:19:24.96 Adding MM1 @21:19:25.02 AS.seek 0.524ml @21:19:43.43 delay 25 seconds @21:20:08.45 ARV.dial :PP1 @21:20:08.84 Adding PP1 @21:20:08.86 delay 2 seconds @21:20:10.92 AS.seek 0.53ml @21:20:17.44 delay 10 seconds @21:20:27.47 ARV.dial :air1 @21:20:27.87 adding ARV air @21:20:27.89 delay 2 seconds @21:20:29.95 AS.seek 0.536ml @21:20:36.29 delay 5 seconds @21:20:41.74 ARV.dial :carrier @21:20:42.19 delay 7 seconds @21:20:49.28 AS.seek 0.594ml @21:21:48.02 delay 5 seconds @21:21:53.04 ATV.dial :ASV @21:21:53.44 delay 2 seconds @21:21:55.52 AS.seek 0.6ml @21:22:01.98 delay 4 seconds @21:22:06.00 ATV.dial ARV @21:22:06.55 delay 2 seconds @21:22:08.57 ARV.dial :carrier @21:22:08.59 delay 2 seconds @21:22:10.66 AS.seek 0.65ml @21:23:01.18 delay 4 seconds @21:23:05.20 ATV.dial PCR @21:23:05.82 ASV.dial :waste1 @21:23:06.59 ARV.dial :air1 @21:23:07.07 Positioning Slug by 0.324ml @21:23:07.15 AS.seek 0.326ml @21:28:33.37 delay 5 seconds @21:28:38.39 ATV.dial ARV @21:28:39.16 ASV.dial :PCRlock @21:28:39.54 delay 5 seconds @21:28:44.57 Running IPc ProfileIPc thermocycling @21:28:44.58 PCR.program ProfileIPc @21:28:44.71 ProfileIPc should complete in < 1:50:49 @21:28:44.73 PCR.run 2:23:31.25 @22:54:05.51 PCR.off @22:54:08.03 PCR for IPc completed -- closed /var/log/esp/IPc19may1521h100ml.pcr @22:54:08.09 Cleaning PCR @22:54:08.10 AFV.select :carrierSolution @22:54:08.21 delay 2 seconds @22:54:10.23 AS.reconfigure ASconfig @22:54:10.28 AS.seek 0.5ml @22:54:20.60 delay 1 second @22:54:21.62 AFV.select :holdingCoil @22:54:21.72 ASV.dial :waste1 @22:54:22.12 ATV.dial :air3 @22:54:23.42 AS.reconfigure ASslow1 @22:54:23.46 AS.seek 0.51ml @22:54:25.73 delay 2 seconds @22:54:27.75 ATV.dial :bleach @22:54:28.38 AS.seek 0.81ml @22:55:29.04 delay 3 seconds @22:55:32.06 ATV.dial :air3 @22:55:32.60 delay 3 seconds @22:55:35.67 AS.seek 0.835ml @22:55:40.98 delay 3 seconds @22:55:44.00 ATV.dial :bleach @22:55:44.61 AS.seek 0.82ml @22:55:47.91 delay 3 seconds @22:55:50.93 ARV.dial :waste @22:55:51.52 delay 3 seconds @22:55:54.54 ATV.dial :ARV @22:55:55.91 AS.reconfigure ASconfig @22:55:55.95 AS.seek 0.47ml @22:56:16.36 AFV.select :carrierSolution @22:56:16.47 delay 2 seconds @22:56:18.49 AS.seek 1ml @22:56:49.21 delay 1 second @22:56:50.23 AFV.select :holdingCoil @22:56:50.34 delay 1 second @22:56:51.36 AS.reconfigure ASfast @22:56:51.41 AS.seek 0ml @22:57:18.76 AFV.select :carrierSolution @22:57:18.87 delay 2 seconds @22:57:20.89 AS.reconfigure ASconfig @22:57:20.94 AS.seek 1ml @22:58:18.72 delay 1 second @22:58:19.74 AFV.select :holdingCoil @22:58:19.85 delay 1 second @22:58:20.87 AS.reconfigure ASfast @22:58:20.92 AS.seek 0ml @22:58:48.22 AFV.select :carrierSolution @22:58:48.33 delay 2 seconds @22:58:50.35 AS.reconfigure ASconfig @22:58:50.40 AS.seek 1ml @22:59:48.15 delay 1 second @22:59:49.17 AFV.select :holdingCoil @22:59:49.28 delay 1 second @22:59:50.30 AS.reconfigure ASfast @22:59:50.35 AS.seek 0ml @23:00:17.66 ARV.dial between carrier and air1 @23:00:18.15 ATV.dial :air3 @23:00:19.46 AS.reconfigure ASslow1 @23:00:19.50 AS.seek 0.01ml @23:00:21.83 delay 2 seconds @23:00:23.85 ATV.dial :bleach @23:00:24.47 AS.seek 0.81ml @23:03:05.60 delay 3 seconds @23:03:08.62 ATV.dial :air3 @23:03:09.18 delay 3 seconds @23:03:12.25 AS.seek 0.835ml @23:03:17.54 delay 3 seconds @23:03:20.56 ATV.dial :bleach @23:03:21.17 AS.seek 0.82ml @23:03:24.47 delay 3 seconds @23:03:27.49 ATV.dial PCR @23:03:27.90 AS.reconfigure ASconfig @23:03:27.95 AS.seek :empty @23:04:15.40 PCR.program :bleachProfile @23:04:15.45 PCR program should complete in < 2:45 @23:04:15.46 PCR.run 10:00 @23:07:11.21 PCR.off @23:07:11.24 AFV.select :carrierSolution @23:07:11.35 delay 2 seconds @23:07:13.37 AS.seek 1ml @23:08:11.19 delay 1 second @23:08:12.21 AFV.select :holdingCoil @23:08:12.32 delay 1 second @23:08:13.34 AS.reconfigure ASfast @23:08:13.39 AS.seek 0ml @23:08:40.72 delay 3 seconds @23:08:43.74 AFV.select :carrierSolution @23:08:43.85 delay 2 seconds @23:08:45.87 AS.reconfigure ASconfig @23:08:45.93 AS.seek 1ml @23:09:43.68 delay 1 second @23:09:44.70 AFV.select :holdingCoil @23:09:44.81 delay 1 second @23:09:45.83 AS.reconfigure ASfast @23:09:45.88 AS.seek 0ml @23:10:13.24 delay 3 seconds @23:10:16.26 AFV.select :carrierSolution @23:10:16.37 delay 2 seconds @23:10:18.39 AS.reconfigure ASconfig @23:10:18.44 AS.seek 1ml @23:11:16.18 delay 1 second @23:11:17.20 AFV.select :holdingCoil @23:11:17.31 delay 1 second @23:11:18.33 AS.reconfigure ASfast @23:11:18.38 AS.seek 0ml @23:11:45.72 delay 3 seconds @23:11:48.74 AFV.select :carrierSolution @23:11:48.85 delay 2 seconds @23:11:50.87 AS.reconfigure ASconfig @23:11:50.93 AS.seek 1ml @23:12:48.69 delay 1 second @23:12:49.71 AFV.select :holdingCoil @23:12:49.82 delay 1 second @23:12:50.84 AS.reconfigure ASfast @23:12:50.89 AS.seek 0ml @23:13:18.21 delay 3 seconds @23:13:21.23 AFV.select :carrierSolution @23:13:21.34 delay 2 seconds @23:13:23.36 AS.reconfigure ASconfig @23:13:23.41 AS.seek 1ml @23:14:21.19 delay 1 second @23:14:22.21 AFV.select :holdingCoil @23:14:22.32 delay 1 second @23:14:23.34 AS.reconfigure ASnorm @23:14:23.39 AS.seek 0ml @23:15:14.09 Setting up PCR for Pp -- writing /var/log/esp/Pp19may1523h100ml.pcr @23:15:14.11 Building PCR Slug from ASV with MM1 mix, PP2 primer @23:15:14.12 Priming with carrier @23:15:14.13 ATV.dial ARV @23:15:15.24 ARV.dial :carrier @23:15:15.59 AS.seek 0.5ml @23:15:41.18 AFV.select :carrierSolution @23:15:41.29 delay 2 seconds @23:15:43.31 AS.reconfigure ASconfig @23:15:43.36 AS.seek 1ml @23:16:12.38 delay 1 second @23:16:13.40 AFV.select :holdingCoil @23:16:13.51 delay 1 second @23:16:14.52 ATV.dial :waste @23:16:16.22 AS.reconfigure ASnorm @23:16:16.27 AS.seek :empty @23:17:07.01 AFV.select :carrierSolution @23:17:07.12 delay 2 seconds @23:17:09.14 AS.reconfigure ASconfig @23:17:09.19 AS.seek 0.5ml @23:17:38.29 delay 1 second @23:17:39.31 AFV.select :holdingCoil @23:17:39.42 Building Slug @23:17:39.43 ATV.dial :ARV @23:17:41.19 delay 2 seconds @23:17:43.21 ARV.dial :air1 @23:17:43.61 delay 2 seconds @23:17:45.68 AS.reconfigure ASslow2 @23:17:45.73 AS.seek 0.506ml @23:17:51.90 delay 2 seconds @23:17:53.92 ARV.dial :MM1 @23:17:54.52 delay 2 seconds @23:17:56.55 Adding MM1 @23:17:56.61 AS.seek 0.524ml @23:18:15.02 delay 25 seconds @23:18:40.04 ARV.dial :PP2 @23:18:40.43 Adding PP2 @23:18:40.45 delay 2 seconds @23:18:42.52 AS.seek 0.53ml @23:18:48.88 delay 10 seconds @23:18:58.91 ARV.dial :air2 @23:18:59.29 adding ARV air @23:18:59.31 delay 2 seconds @23:19:01.38 AS.seek 0.536ml @23:19:07.61 delay 5 seconds @23:19:12.63 ARV.dial :carrier @23:19:13.91 delay 7 seconds @23:19:21.00 AS.seek 0.594ml @23:20:19.63 delay 5 seconds @23:20:24.65 ATV.dial :ASV @23:20:25.05 delay 2 seconds @23:20:27.12 AS.seek 0.6ml @23:20:33.47 delay 4 seconds @23:20:37.49 ATV.dial ARV @23:20:38.04 delay 2 seconds @23:20:40.06 ARV.dial :carrier @23:20:40.08 delay 2 seconds @23:20:42.15 AS.seek 0.65ml @23:21:32.79 delay 4 seconds @23:21:36.81 ATV.dial PCR @23:21:37.44 ASV.dial :waste1 @23:21:37.45 ARV.dial :air1 @23:21:37.87 Positioning Slug by 0.324ml @23:21:37.94 AS.seek 0.326ml @23:27:03.96 delay 5 seconds @23:27:08.98 ATV.dial ARV @23:27:09.76 ASV.dial :PCRlock @23:27:10.15 delay 5 seconds @23:27:15.18 Running Pp Profile60C350 thermocycling @23:27:15.19 PCR.program Profile60C350 @23:27:15.31 Profile60C350 should complete in < 1:48:05 @23:27:15.33 PCR.run 2:20:06.25 @00:50:35.21CEST16-May-19 PCR.off @00:50:37.20 PCR for Pp completed -- closed /var/log/esp/Pp19may1523h100ml.pcr @00:50:37.25 Cleaning PCR @00:50:37.26 AFV.select :carrierSolution @00:50:37.37 delay 2 seconds @00:50:39.39 AS.reconfigure ASconfig @00:50:39.44 AS.seek 0.5ml @00:50:49.74 delay 1 second @00:50:50.76 AFV.select :holdingCoil @00:50:50.86 ASV.dial :waste1 @00:50:51.26 ATV.dial :air3 @00:50:52.55 AS.reconfigure ASslow1 @00:50:52.59 AS.seek 0.51ml @00:50:54.87 delay 2 seconds @00:50:56.89 ATV.dial :bleach @00:50:57.52 AS.seek 0.81ml @00:51:58.11 delay 3 seconds @00:52:01.13 ATV.dial :air3 @00:52:01.69 delay 3 seconds @00:52:05.26 AS.seek 0.835ml @00:52:10.54 delay 3 seconds @00:52:13.56 ATV.dial :bleach @00:52:14.19 AS.seek 0.82ml @00:52:17.52 delay 3 seconds @00:52:20.54 ARV.dial :waste @00:52:21.15 delay 3 seconds @00:52:24.17 ATV.dial :ARV @00:52:25.08 AS.reconfigure ASconfig @00:52:25.12 AS.seek 0.47ml @00:52:45.52 AFV.select :carrierSolution @00:52:45.63 delay 2 seconds @00:52:47.65 AS.seek 1ml @00:53:18.38 delay 1 second @00:53:19.40 AFV.select :holdingCoil @00:53:19.51 delay 1 second @00:53:20.53 AS.reconfigure ASfast @00:53:20.58 AS.seek 0ml @00:53:47.93 AFV.select :carrierSolution @00:53:48.04 delay 2 seconds @00:53:50.06 AS.reconfigure ASconfig @00:53:50.11 AS.seek 1ml @00:54:47.86 delay 1 second @00:54:48.88 AFV.select :holdingCoil @00:54:48.99 delay 1 second @00:54:50.01 AS.reconfigure ASfast @00:54:50.06 AS.seek 0ml @00:55:17.36 AFV.select :carrierSolution @00:55:17.47 delay 2 seconds @00:55:19.49 AS.reconfigure ASconfig @00:55:19.54 AS.seek 1ml @00:56:17.29 delay 1 second @00:56:18.31 AFV.select :holdingCoil @00:56:18.42 delay 1 second @00:56:19.44 AS.reconfigure ASfast @00:56:19.49 AS.seek 0ml @00:56:46.82 ARV.dial between carrier and air1 @00:56:47.30 ATV.dial :air3 @00:56:48.61 AS.reconfigure ASslow1 @00:56:48.65 AS.seek 0.01ml @00:56:50.99 delay 2 seconds @00:56:53.01 ATV.dial :bleach @00:56:53.63 AS.seek 0.81ml @00:59:34.77 delay 3 seconds @00:59:37.79 ATV.dial :air3 @00:59:38.33 delay 3 seconds @00:59:41.40 AS.seek 0.835ml @00:59:46.70 delay 3 seconds @00:59:49.72 ATV.dial :bleach @00:59:50.33 AS.seek 0.82ml @00:59:53.66 delay 3 seconds @00:59:56.68 ATV.dial PCR @00:59:57.08 AS.reconfigure ASconfig @00:59:57.13 AS.seek :empty @01:00:45.57 PCR.program :bleachProfile @01:00:45.62 PCR program should complete in < 2:45 @01:00:45.63 PCR.run 10:00 @01:03:41.40 PCR.off @01:03:41.43 AFV.select :carrierSolution @01:03:41.53 delay 2 seconds @01:03:43.55 AS.seek 1ml @01:04:41.38 delay 1 second @01:04:42.40 AFV.select :holdingCoil @01:04:42.51 delay 1 second @01:04:43.53 AS.reconfigure ASfast @01:04:43.58 AS.seek 0ml @01:05:10.90 delay 3 seconds @01:05:13.92 AFV.select :carrierSolution @01:05:14.03 delay 2 seconds @01:05:16.05 AS.reconfigure ASconfig @01:05:16.11 AS.seek 1ml @01:06:13.83 delay 1 second @01:06:14.85 AFV.select :holdingCoil @01:06:14.97 delay 1 second @01:06:15.99 AS.reconfigure ASfast @01:06:16.04 AS.seek 0ml @01:06:43.36 delay 3 seconds @01:06:46.38 AFV.select :carrierSolution @01:06:46.49 delay 2 seconds @01:06:48.51 AS.reconfigure ASconfig @01:06:48.56 AS.seek 1ml @01:07:46.29 delay 1 second @01:07:47.31 AFV.select :holdingCoil @01:07:47.41 delay 1 second @01:07:48.44 AS.reconfigure ASfast @01:07:48.49 AS.seek 0ml @01:08:15.80 delay 3 seconds @01:08:18.82 AFV.select :carrierSolution @01:08:18.93 delay 2 seconds @01:08:20.95 AS.reconfigure ASconfig @01:08:21.01 AS.seek 1ml @01:09:18.74 delay 1 second @01:09:19.76 AFV.select :holdingCoil @01:09:19.87 delay 1 second @01:09:20.89 AS.reconfigure ASfast @01:09:20.94 AS.seek 0ml @01:09:48.27 delay 3 seconds @01:09:51.29 AFV.select :carrierSolution @01:09:51.40 delay 2 seconds @01:09:53.42 AS.reconfigure ASconfig @01:09:53.98 AS.seek 1ml @01:10:51.76 delay 1 second @01:10:52.78 AFV.select :holdingCoil @01:10:52.89 delay 1 second @01:10:53.91 AS.reconfigure ASnorm @01:10:53.96 AS.seek 0ml @01:11:44.67 Setting up PCR for Ss -- writing /var/log/esp/Ss19may1601h100ml.pcr @01:11:44.68 Building PCR Slug from ASV with MM2 mix, PP4 primer @01:11:44.70 Priming with carrier ATV.dial ARV @01:11:45.33 ARV.dial :carrier @01:11:45.67 AS.seek 0.5ml @01:12:11.22 AFV.select :carrierSolution @01:12:11.33 delay 2 seconds @01:12:13.35 AS.reconfigure ASconfig @01:12:13.40 AS.seek 1ml @01:12:42.40 delay 1 second @01:12:43.42 AFV.select :holdingCoil @01:12:43.53 delay 1 second @01:12:44.54 ATV.dial :waste @01:12:46.24 AS.reconfigure ASnorm @01:12:46.29 AS.seek :empty @01:13:37.02 AFV.select :carrierSolution @01:13:37.13 delay 2 seconds @01:13:39.15 AS.reconfigure ASconfig @01:13:39.20 AS.seek 0.5ml @01:14:08.29 delay 1 second @01:14:09.31 AFV.select :holdingCoil @01:14:09.42 Building Slug @01:14:09.43 ATV.dial :ARV @01:14:11.10 delay 2 seconds @01:14:13.12 ARV.dial :air1 @01:14:13.52 delay 2 seconds @01:14:15.59 AS.reconfigure ASslow2 @01:14:15.64 AS.seek 0.506ml @01:14:22.05 delay 2 seconds @01:14:24.07 ARV.dial :MM2 @01:14:25.55 delay 2 seconds @01:14:27.58 Adding MM2 @01:14:27.63 AS.seek 0.524ml @01:14:46.08 delay 25 seconds @01:15:11.10 ARV.dial :PP4 @01:15:11.49 Adding PP4 @01:15:11.50 delay 2 seconds @01:15:13.58 AS.seek 0.53ml @01:15:19.79 delay 10 seconds @01:15:29.82 ARV.dial :air3 @01:15:30.21 adding ARV air @01:15:30.23 delay 2 seconds @01:15:32.30 AS.seek 0.536ml @01:15:38.51 delay 5 seconds @01:15:43.53 ARV.dial :carrier @01:15:44.80 delay 7 seconds @01:15:51.88 AS.seek 0.594ml @01:16:50.47 delay 5 seconds @01:16:55.49 ATV.dial :ASV @01:16:55.90 delay 2 seconds @01:16:57.97 AS.seek 0.6ml @01:17:04.27 delay 4 seconds @01:17:08.29 ATV.dial ARV @01:17:08.83 delay 2 seconds @01:17:10.85 ARV.dial :carrier @01:17:10.86 delay 2 seconds @01:17:12.93 AS.seek 0.65ml @01:18:03.40 delay 4 seconds @01:18:07.42 ATV.dial PCR @01:18:08.05 ASV.dial :waste1 @01:18:08.06 ARV.dial :air1 @01:18:08.45 Positioning Slug by 0.324ml @01:18:08.53 AS.seek 0.326ml @01:23:34.54 delay 5 seconds @01:23:39.56 ATV.dial ARV @01:23:40.44 ASV.dial :PCRlock @01:23:40.83 delay 5 seconds @01:23:45.86 Running Ss Profile60C350 thermocycling @01:23:45.87 PCR.program Profile60C350 @01:23:46.00 Profile60C350 should complete in < 1:48:05 @01:23:46.01 PCR.run 2:20:06.25 @02:47:08.15 PCR.off @02:47:10.13 PCR for Ss completed -- closed /var/log/esp/Ss19may1601h100ml.pcr @02:47:10.19 Cleaning PCR @02:47:10.20 AFV.select :carrierSolution @02:47:10.31 delay 2 seconds @02:47:12.33 AS.reconfigure ASconfig @02:47:12.38 AS.seek 0.5ml @02:47:22.68 delay 1 second @02:47:23.70 AFV.select :holdingCoil @02:47:23.80 ASV.dial :waste1 @02:47:24.19 ATV.dial :air3 @02:47:25.50 AS.reconfigure ASslow1 @02:47:25.54 AS.seek 0.51ml @02:47:27.80 delay 2 seconds @02:47:29.82 ATV.dial :bleach @02:47:30.94 AS.seek 0.81ml @02:48:31.52 delay 3 seconds @02:48:34.54 ATV.dial :air3 @02:48:35.08 delay 3 seconds @02:48:38.15 AS.seek 0.835ml @02:48:43.44 delay 3 seconds @02:48:46.46 ATV.dial :bleach @02:48:47.07 AS.seek 0.82ml @02:48:50.51 delay 3 seconds @02:48:53.53 ARV.dial :waste @02:48:54.15 delay 3 seconds @02:48:57.17 ATV.dial :ARV @02:48:58.08 AS.reconfigure ASconfig @02:48:58.12 AS.seek 0.47ml @02:49:18.54 AFV.select :carrierSolution @02:49:18.65 delay 2 seconds @02:49:20.67 AS.seek 1ml @02:49:51.44 delay 1 second @02:49:52.46 AFV.select :holdingCoil @02:49:52.57 delay 1 second @02:49:53.59 AS.reconfigure ASfast @02:49:53.64 AS.seek 0ml @02:50:20.97 AFV.select :carrierSolution @02:50:21.08 delay 2 seconds @02:50:23.10 AS.reconfigure ASconfig @02:50:23.15 AS.seek 1ml @02:51:20.90 delay 1 second @02:51:21.92 AFV.select :holdingCoil @02:51:22.03 delay 1 second @02:51:23.05 AS.reconfigure ASfast @02:51:23.10 AS.seek 0ml @02:51:50.41 AFV.select :carrierSolution @02:51:50.52 delay 2 seconds @02:51:52.54 AS.reconfigure ASconfig @02:51:52.59 AS.seek 1ml @02:52:50.33 delay 1 second @02:52:51.35 AFV.select :holdingCoil @02:52:51.46 delay 1 second @02:52:52.48 AS.reconfigure ASfast @02:52:52.53 AS.seek 0ml @02:53:19.83 ARV.dial between carrier and air1 @02:53:20.43 ATV.dial :air3 @02:53:21.72 AS.reconfigure ASslow1 @02:53:21.76 AS.seek 0.01ml @02:53:24.05 delay 2 seconds @02:53:26.07 ATV.dial :bleach @02:53:26.68 AS.seek 0.81ml @02:56:07.86 delay 3 seconds @02:56:10.88 ATV.dial :air3 @02:56:11.43 delay 3 seconds @02:56:14.50 AS.seek 0.835ml @02:56:19.80 delay 3 seconds @02:56:22.82 ATV.dial :bleach @02:56:23.43 AS.seek 0.82ml @02:56:26.74 delay 3 seconds @02:56:29.76 ATV.dial PCR @02:56:30.16 AS.reconfigure ASconfig @02:56:30.21 AS.seek :empty @02:57:17.68 PCR.program :bleachProfile @02:57:17.73 PCR program should complete in < 2:45 @02:57:17.74 PCR.run 10:00 @03:00:13.50 PCR.off @03:00:13.53 AFV.select :carrierSolution @03:00:13.63 delay 2 seconds @03:00:15.65 AS.seek 1ml @03:01:13.47 delay 1 second @03:01:14.49 AFV.select :holdingCoil @03:01:14.60 delay 1 second @03:01:15.62 AS.reconfigure ASfast @03:01:15.67 AS.seek 0ml @03:01:43.01 delay 3 seconds @03:01:46.03 AFV.select :carrierSolution @03:01:46.14 delay 2 seconds @03:01:48.16 AS.reconfigure ASconfig @03:01:48.21 AS.seek 1ml @03:02:45.96 delay 1 second @03:02:46.98 AFV.select :holdingCoil @03:02:47.09 delay 1 second @03:02:48.11 AS.reconfigure ASfast @03:02:48.16 AS.seek 0ml @03:03:15.51 delay 3 seconds @03:03:18.53 AFV.select :carrierSolution @03:03:18.64 delay 2 seconds @03:03:20.66 AS.reconfigure ASconfig @03:03:20.72 AS.seek 1ml @03:04:18.44 delay 1 second @03:04:19.46 AFV.select :holdingCoil @03:04:19.57 delay 1 second @03:04:20.59 AS.reconfigure ASfast @03:04:20.64 AS.seek 0ml @03:04:47.94 delay 3 seconds @03:04:50.96 AFV.select :carrierSolution @03:04:51.07 delay 2 seconds @03:04:53.10 AS.reconfigure ASconfig @03:04:53.14 AS.seek 1ml @03:05:50.86 delay 1 second @03:05:51.88 AFV.select :holdingCoil @03:05:51.99 delay 1 second @03:05:53.01 AS.reconfigure ASfast @03:05:53.57 AS.seek 0ml @03:06:20.90 delay 3 seconds @03:06:23.92 AFV.select :carrierSolution @03:06:24.03 delay 2 seconds @03:06:26.05 AS.reconfigure ASconfig @03:06:26.11 AS.seek 1ml @03:07:23.83 delay 1 second @03:07:24.85 AFV.select :holdingCoil @03:07:24.96 delay 1 second @03:07:25.98 AS.reconfigure ASnorm @03:07:26.03 AS.seek 0ml @03:08:16.73 Setting up PCR for Mm -- writing /var/log/esp/Mm19may1603h100ml.pcr @03:08:16.74 Building PCR Slug from ASV with MM2 mix, PP3 primer @03:08:16.76 Priming with carrier ATV.dial ARV @03:08:17.38 ARV.dial :carrier @03:08:17.71 AS.seek 0.5ml @03:08:43.29 AFV.select :carrierSolution @03:08:43.40 delay 2 seconds @03:08:45.42 AS.reconfigure ASconfig @03:08:45.47 AS.seek 1ml @03:09:14.47 delay 1 second @03:09:15.49 AFV.select :holdingCoil @03:09:15.60 delay 1 second @03:09:16.61 ATV.dial :waste @03:09:18.32 AS.reconfigure ASnorm @03:09:18.37 AS.seek :empty @03:10:09.12 AFV.select :carrierSolution @03:10:09.23 delay 2 seconds @03:10:11.25 AS.reconfigure ASconfig @03:10:11.30 AS.seek 0.5ml @03:10:40.41 delay 1 second @03:10:41.43 AFV.select :holdingCoil @03:10:41.54 Building Slug @03:10:41.55 ATV.dial :ARV @03:10:43.22 delay 2 seconds @03:10:45.24 ARV.dial :air1 @03:10:45.63 delay 2 seconds @03:10:47.70 AS.reconfigure ASslow2 @03:10:47.75 AS.seek 0.506ml @03:10:54.05 delay 2 seconds @03:10:56.07 ARV.dial :MM2 @03:10:57.57 delay 2 seconds @03:10:59.60 Adding MM2 @03:10:59.66 AS.seek 0.524ml @03:11:17.96 delay 25 seconds @03:11:42.98 ARV.dial :PP3 @03:11:43.37 Adding PP3 @03:11:43.38 delay 2 seconds @03:11:45.46 AS.seek 0.53ml @03:11:51.94 delay 10 seconds @03:12:01.97 ARV.dial :air2 @03:12:02.37 adding ARV air @03:12:02.38 delay 2 seconds @03:12:04.45 AS.seek 0.536ml @03:12:10.90 delay 5 seconds @03:12:15.92 ARV.dial :carrier @03:12:17.19 delay 7 seconds @03:12:24.28 AS.seek 0.594ml @03:13:22.88 delay 5 seconds @03:13:27.90 ATV.dial :ASV @03:13:28.30 delay 2 seconds @03:13:30.37 AS.seek 0.6ml @03:13:36.63 delay 4 seconds @03:13:40.65 ATV.dial ARV @03:13:41.19 delay 2 seconds @03:13:43.21 ARV.dial :carrier @03:13:43.22 delay 2 seconds @03:13:45.30 AS.seek 0.65ml @03:14:35.94 delay 4 seconds @03:14:39.96 ATV.dial PCR @03:14:40.58 ASV.dial :waste1 @03:14:40.60 ARV.dial :air1 @03:14:41.00 Positioning Slug by 0.324ml @03:14:41.08 AS.seek 0.326ml @03:20:07.11 delay 5 seconds @03:20:12.13 ATV.dial ARV @03:20:12.91 ASV.dial :PCRlock @03:20:13.30 delay 5 seconds @03:20:18.33 Running Mm Profile60C350 thermocycling @03:20:18.34 PCR.program Profile60C350 @03:20:18.46 Profile60C350 should complete in < 1:48:05 @03:20:18.48 PCR.run 2:20:06.25 @04:43:38.30 PCR.off @04:43:40.29 PCR for Mm completed -- closed /var/log/esp/Mm19may1603h100ml.pcr @04:43:40.31 Full MFB Decontamination Decon extraction column and decon AIV @04:43:40.33 SPE.hold 2:46:40,55C @04:43:40.37 ASV.dial :SPEdelivery @04:43:41.02 delay 20 seconds @04:44:01.05 AFV.select :carrierSolution @04:44:01.16 delay 2 seconds @04:44:03.18 AS.reconfigure ASconfig @04:44:03.23 AS.seek 0.5ml @04:44:13.54 delay 1 second @04:44:14.56 AFV.select :holdingCoil @04:44:14.66 ATV.dial :air1 @04:44:17.36 AS.reconfigure ASslow1 @04:44:17.40 AS.seek 0.52ml @04:44:21.71 ATV.dial :ASV @04:44:23.44 ASV.dial :SPErecovery @04:44:24.11 AS.seek 0.62ml @04:44:44.51 ATV.dial :waste @04:44:46.63 AS.reconfigure ASnorm @04:44:46.67 AS.seek :empty @04:45:18.30 AFV.select :holdingCoil @04:45:18.31 ATV.dial :waste @04:45:18.33 AS.reconfigure ASconfig @04:45:18.37 AS.seek 0ml @04:45:18.65 AFV.select :carrierSolution @04:45:18.76 delay 2 seconds @04:45:20.78 AS.seek 0.5ml @04:45:49.79 delay 1 second @04:45:50.81 AFV.select :holdingCoil @04:45:50.91 ATV.dial :air3 @04:45:51.66 AS.reconfigure ASslow1 @04:45:51.70 AS.seek 0.52ml @04:45:55.93 ATV.dial :air3 @04:45:56.00 AS.seek 0.53ml @04:45:58.32 delay 2 seconds @04:46:00.34 ATV.dial :bleach @04:46:00.83 AS.seek 0.71ml @04:46:37.29 delay 3 seconds @04:46:40.31 ATV.dial :air3 @04:46:40.90 delay 3 seconds @04:46:43.97 AS.seek 0.735ml @04:46:49.27 delay 3 seconds @04:46:52.29 ATV.dial :bleach @04:46:52.93 AS.seek 0.72ml @04:46:56.35 delay 3 seconds @04:46:59.37 ATV.dial ASV @04:47:00.49 ASV.dial :SPErecovery @04:47:00.56 AS.seek 0.365ml @04:48:12.26 ATV.dial :waste @04:48:14.07 AS.reconfigure ASnorm @04:48:14.12 AS.seek :empty @04:48:32.90 ATV.dial :air3 @04:48:33.60 AS.reconfigure ASslow1 @04:48:33.64 AS.seek 0.009ml @04:48:35.99 delay 2 seconds @04:48:38.01 ATV.dial :bleach @04:48:38.49 AS.seek 0.579ml @04:50:33.40 delay 3 seconds @04:50:36.42 ATV.dial :air3 @04:50:36.98 delay 3 seconds @04:50:40.05 AS.seek 0.604ml @04:50:45.40 delay 3 seconds @04:50:48.42 ATV.dial :bleach @04:50:49.04 AS.seek 0.589ml @04:50:52.33 delay 3 seconds @04:50:55.35 ATV.dial :ASV @04:50:56.40 ASV.dial :wasteR @04:50:56.78 AS.seek 0ml @04:52:55.52 delay 1:00 @04:53:55.55 ATV.dial ASV @04:53:55.56 AFV.select :carrierSolution @04:53:55.67 delay 2 seconds @04:53:57.69 AS.reconfigure ASconfig @04:53:57.74 AS.seek 1ml @04:54:55.51 delay 1 second @04:54:56.53 AFV.select :holdingCoil @04:54:56.64 delay 1 second @04:54:57.65 ASV.dial :wasteR @04:54:57.67 AS.reconfigure ASslow1 @04:54:57.72 AS.seek 0ml @04:58:19.07 delay 10 seconds @04:58:29.10 ATV.dial :air3 @04:58:30.64 AS.seek 0.01ml @04:58:32.93 delay 2 seconds @04:58:34.95 ATV.dial :bleach @04:58:35.57 AS.seek 0.58ml @05:00:30.48 delay 3 seconds @05:00:33.50 ATV.dial :air3 @05:00:34.05 delay 3 seconds @05:00:37.12 AS.seek 0.605ml @05:00:42.51 delay 3 seconds @05:00:45.53 ATV.dial :bleach @05:00:46.14 AS.seek 0.59ml @05:00:49.44 delay 3 seconds @05:00:52.46 ATV.dial ASV @05:00:53.52 ASV.dial :SPEdelivery @05:00:54.41 AS.seek 0ml @05:02:53.30 delay 1:00 @05:03:53.33 AFV.select :carrierSolution @05:03:53.44 delay 2 seconds @05:03:55.46 AS.reconfigure ASconfig @05:03:55.51 AS.seek 1ml @05:04:53.26 delay 1 second @05:04:54.28 AFV.select :holdingCoil @05:04:54.39 delay 1 second @05:04:55.40 ATV.dial ASV @05:04:55.41 ASV.dial :SPEdelivery @05:04:55.43 AS.reconfigure ASslow1 @05:04:55.48 AS.seek 0ml @05:08:16.87 delay 10 seconds @05:08:26.90 ATV.dial :air2 @05:08:27.75 AS.reconfigure ASslow0 @05:08:27.79 AS.seek 0.8ml @05:09:48.55 delay 3 seconds @05:09:51.57 ATV.dial :ASV @05:09:52.57 ASV.dial :SPEdelivery @05:09:52.59 AS.reconfigure ASslow1 @05:09:52.63 AS.seek 0.03ml @05:12:27.74 delay 20 seconds @05:12:47.77 ATV.dial :waste,via: :air3 @05:12:49.55 AS.reconfigure ASconfig @05:12:49.60 AS.seek :empty @05:12:51.68 SPE.coast @05:12:51.75 Removing Bleach from Mixing Coil @05:12:51.76 AFV.select :carrierSolution @05:12:51.86 delay 2 seconds @05:12:53.88 AS.seek 0.5ml @05:13:22.98 delay 1 second @05:13:24.00 AFV.select :holdingCoil @05:13:24.10 ATV.dial :ASV @05:13:25.85 ASV.dial :SPErecovery @05:13:26.49 AS.reconfigure ASslow1 @05:13:26.53 AS.seek 0.73ml @05:14:13.07 ASV.dial :wasteD @05:14:13.64 AS.reconfigure ASnorm @05:14:13.68 AS.seek 0ml @05:14:50.79 AFV.select :carrierSolution @05:14:50.90 delay 2 seconds @05:14:52.92 AS.reconfigure ASconfig @05:14:52.97 AS.seek 0.5ml @05:15:22.02 delay 1 second @05:15:23.04 AFV.select :holdingCoil @05:15:23.14 ATV.dial ASV @05:15:23.16 ASV.dial :SPErecovery @05:15:23.79 AS.reconfigure ASslow0 @05:15:23.83 AS.seek 0.3ml @05:15:44.24 ATV.dial :air2 @05:15:45.29 AS.reconfigure ASslow1 @05:15:45.33 AS.seek 0.32ml @05:15:49.71 ATV.dial ASV @05:15:50.75 AS.reconfigure ASslow0 @05:15:50.79 AS.seek 0.57ml @05:16:16.25 ASV.dial :wasteD @05:16:16.83 AS.reconfigure ASnorm @05:16:16.87 AS.seek 0ml @05:16:45.93 AFV.select :carrierSolution @05:16:46.04 delay 2 seconds @05:16:48.06 AS.reconfigure ASconfig @05:16:48.11 AS.seek 0.5ml @05:17:17.13 delay 1 second @05:17:18.15 AFV.select :holdingCoil @05:17:18.25 ATV.dial ASV @05:17:18.27 ASV.dial :SPErecovery @05:17:18.89 AS.reconfigure ASslow0 @05:17:18.93 AS.seek 0.3ml @05:17:39.33 ATV.dial :air2 @05:17:40.38 AS.reconfigure ASslow1 @05:17:40.42 AS.seek 0.32ml @05:17:44.80 ATV.dial ASV @05:17:45.86 AS.reconfigure ASslow0 @05:17:45.90 AS.seek 0.57ml @05:18:11.37 ASV.dial :wasteD @05:18:12.03 AS.reconfigure ASnorm @05:18:12.07 AS.seek 0ml @05:18:41.13 ATV.dial :air2 @05:18:42.13 AS.reconfigure ASslow0 @05:18:42.17 AS.seek 0.3ml @05:19:12.73 ATV.dial :ASV @05:19:13.73 ASV.dial :wasteD @05:19:13.75 AS.reconfigure ASconfig @05:19:13.79 AS.seek 0.05ml @05:19:28.49 ATV.dial :waste @05:19:30.39 AS.seek 0ml @05:19:33.55 ASV.dial :SPEdelivery @05:19:34.54 deconLysateINT @05:19:34.55 ATV.dial :air3 @05:19:35.75 AS.reconfigure ASslow1 @05:19:35.80 AS.seek 0.01ml @05:19:38.13 delay 2 seconds @05:19:40.15 ATV.dial :bleach @05:19:40.61 AS.seek 0.96ml @05:22:51.91 delay 3 seconds @05:22:54.93 ATV.dial :air3 @05:22:55.49 delay 3 seconds @05:22:58.56 AS.seek 0.985ml @05:23:03.91 delay 3 seconds @05:23:06.93 ATV.dial :bleach @05:23:07.55 AS.seek 0.97ml @05:23:10.87 delay 3 seconds @05:23:13.89 ATV.dial AIV @05:23:15.18 AIV.dial :coilToMFB @05:23:15.19 AWV.select :waste @05:23:15.20 AFV.select :carrierSolution @05:23:15.31 delay 2 seconds @05:23:17.33 AS.reconfigure ASconfig @05:23:17.38 AS.seek 1ml @05:23:19.41 delay 1 second @05:23:20.43 AFV.select :holdingCoil @05:23:20.54 delay 1 second @05:23:21.56 AS.reconfigure ASslow0 @05:23:21.60 AS.seek :empty @05:25:02.57 AFV.select :carrierSolution @05:25:02.68 delay 2 seconds @05:25:04.70 AS.reconfigure ASconfig @05:25:04.75 AS.seek 1ml @05:26:02.58 delay 1 second @05:26:03.60 AFV.select :holdingCoil @05:26:03.71 delay 1 second @05:26:04.73 AS.reconfigure ASslow0 @05:26:04.77 AS.seek :empty @05:27:45.76 AFV.select :carrierSolution @05:27:45.87 delay 2 seconds @05:27:47.89 AS.reconfigure ASconfig @05:27:47.94 AS.seek 1ml @05:28:45.74 delay 1 second @05:28:46.76 AFV.select :holdingCoil @05:28:46.87 delay 1 second @05:28:47.89 AS.reconfigure ASslow0 @05:28:47.93 AS.seek :empty @05:30:28.90 ATV.dial :air2 @05:30:29.57 AS.reconfigure ASslow1 @05:30:29.61 AS.seek 0.009ml @05:30:31.98 AS.reconfigure ASslow0 @05:30:32.03 AS.seek 1ml @05:32:12.01 ATV.dial AIV @05:32:12.89 AS.seek 0.02ml @05:33:51.76 ATV.dial :waste @05:33:53.44 AS.seek 0ml @05:33:55.74 AWV.select :air @05:33:55.89 Cleaning PCR @05:33:55.90 AFV.select :carrierSolution @05:33:56.00 delay 2 seconds @05:33:58.02 AS.reconfigure ASconfig @05:33:58.07 AS.seek 0.5ml @05:34:27.10 delay 1 second @05:34:28.12 AFV.select :holdingCoil @05:34:28.22 ASV.dial :waste1 @05:34:28.58 ATV.dial :air3 @05:34:29.27 AS.reconfigure ASslow1 @05:34:29.31 AS.seek 0.51ml @05:34:31.58 delay 2 seconds @05:34:33.60 ATV.dial :bleach @05:34:34.05 AS.seek 0.81ml @05:35:34.65 delay 3 seconds @05:35:37.67 ATV.dial :air3 @05:35:38.21 delay 3 seconds @05:35:41.28 AS.seek 0.835ml @05:35:46.57 delay 3 seconds @05:35:49.59 ATV.dial :bleach @05:35:50.19 AS.seek 0.82ml @05:35:53.49 delay 3 seconds @05:35:56.51 ARV.dial :waste @05:35:57.12 delay 3 seconds @05:36:00.14 ATV.dial :ARV @05:36:01.14 AS.reconfigure ASconfig @05:36:01.18 AS.seek 0.47ml @05:36:21.57 AFV.select :carrierSolution @05:36:21.68 delay 2 seconds @05:36:23.70 AS.seek 1ml @05:36:54.46 delay 1 second @05:36:55.48 AFV.select :holdingCoil @05:36:55.59 delay 1 second @05:36:56.61 AS.reconfigure ASfast @05:36:56.66 AS.seek 0ml @05:37:23.99 AFV.select :carrierSolution @05:37:24.10 delay 2 seconds @05:37:26.12 AS.reconfigure ASconfig @05:37:26.17 AS.seek 1ml @05:38:23.94 delay 1 second @05:38:24.96 AFV.select :holdingCoil @05:38:25.07 delay 1 second @05:38:26.09 AS.reconfigure ASfast @05:38:26.14 AS.seek 0ml @05:38:53.49 AFV.select :carrierSolution @05:38:53.60 delay 2 seconds @05:38:56.10 AS.reconfigure ASconfig @05:38:56.18 AS.seek 1ml @05:39:53.96 delay 1 second @05:39:54.98 AFV.select :holdingCoil @05:39:55.09 delay 1 second @05:39:56.11 AS.reconfigure ASfast @05:39:56.16 AS.seek 0ml @05:40:23.52 ARV.dial between carrier and air1 @05:40:24.01 ATV.dial :air3 @05:40:25.30 AS.reconfigure ASslow1 @05:40:25.34 AS.seek 0.01ml @05:40:27.69 delay 2 seconds @05:40:29.71 ATV.dial :bleach @05:40:30.32 AS.seek 0.81ml @05:43:11.44 delay 3 seconds @05:43:14.46 ATV.dial :air3 @05:43:15.01 delay 3 seconds @05:43:18.09 AS.seek 0.835ml @05:43:23.37 delay 3 seconds @05:43:26.39 ATV.dial :bleach @05:43:27.00 AS.seek 0.82ml @05:43:30.30 delay 3 seconds @05:43:33.32 ATV.dial PCR @05:43:33.73 AS.reconfigure ASconfig @05:43:33.78 AS.seek :empty @05:44:21.24 PCR.program :bleachProfile @05:44:21.29 PCR program should complete in < 2:45 @05:44:21.30 PCR.run 10:00 @05:47:23.09 PCR.off @05:47:23.12 AFV.select :carrierSolution @05:47:23.23 delay 2 seconds @05:47:25.25 AS.seek 1ml @05:48:23.07 delay 1 second @05:48:24.09 AFV.select :holdingCoil @05:48:24.20 delay 1 second @05:48:25.22 AS.reconfigure ASfast @05:48:25.27 AS.seek 0ml @05:48:52.58 delay 3 seconds @05:48:55.60 AFV.select :carrierSolution @05:48:55.71 delay 2 seconds @05:48:57.73 AS.reconfigure ASconfig @05:48:57.79 AS.seek 1ml @05:49:55.54 delay 1 second @05:49:56.56 AFV.select :holdingCoil @05:49:56.67 delay 1 second @05:49:57.69 AS.reconfigure ASfast @05:49:57.74 AS.seek 0ml @05:50:25.08 delay 3 seconds @05:50:28.10 AFV.select :carrierSolution @05:50:28.21 delay 2 seconds @05:50:30.23 AS.reconfigure ASconfig @05:50:30.29 AS.seek 1ml @05:51:28.04 delay 1 second @05:51:29.06 AFV.select :holdingCoil @05:51:29.17 delay 1 second @05:51:30.19 AS.reconfigure ASfast @05:51:30.24 AS.seek 0ml @05:51:57.57 delay 3 seconds @05:52:00.59 AFV.select :carrierSolution @05:52:00.70 delay 2 seconds @05:52:02.72 AS.reconfigure ASconfig @05:52:02.78 AS.seek 1ml @05:53:00.52 delay 1 second @05:53:01.54 AFV.select :holdingCoil @05:53:01.65 delay 1 second @05:53:02.67 AS.reconfigure ASfast @05:53:02.72 AS.seek 0ml @05:53:30.04 delay 3 seconds @05:53:33.06 AFV.select :carrierSolution @05:53:33.17 delay 2 seconds @05:53:35.20 AS.reconfigure ASconfig @05:53:35.25 AS.seek 1ml @05:54:33.02 delay 1 second @05:54:34.04 AFV.select :holdingCoil @05:54:34.15 delay 1 second @05:54:35.17 AS.reconfigure ASnorm @05:54:35.22 AS.seek 0ml @05:55:25.91 AFV.select :carrierSolution @05:55:26.02 delay 2 seconds @05:55:28.04 AS.reconfigure ASconfig @05:55:28.09 AS.seek 1ml @05:56:25.83 delay 1 second @05:56:26.85 AFV.select :holdingCoil @05:56:26.96 delay 1 second @05:56:27.97 ATV.dial :waste @05:56:29.22 AS.reconfigure ASnorm @05:56:29.26 AS.seek 0.5ml @05:56:54.82 ATV.dial :waste2 @05:56:56.14 AS.seek 0ml @05:57:21.66
Asleep after completing 190515totalsampletest assays @05:57:21.67 GoodNews.email "{1 => 3, 2 => 0}\nCan@05:56:10, 21.3C, 28% humidity, 14.8psia, 12.055V, 0.474A, 0.474A avg, 5.72W",Subject: "Asleep after completing 190515totalsampletest assays" @05:57:21.69
Mission runtime was 13:01:11.322596 @18:17:57.91CEST16-May-19
ESP2 v3.30 I2C Gateway v3.06 for core bus @18:17:59.60 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @18:17:59.86 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @18:18:04.18 Polling Can every 10:00 @18:18:05.93 SPE.coast @18:18:05.97 SPE.configure SPEconfig @18:18:06.05 AS.reconfigure ASconfig @18:18:06.09 AS.hold @18:18:06.15 AS.configure ASconfig @18:18:06.24 SS.reconfigure SSconfig @18:18:06.28 SS.hold @18:18:06.32 SS.configure SSconfig @18:18:06.40 CC.reconfigure CCconfig @18:18:06.44 CC.hold @18:18:06.50 CC.configure CCconfig @18:18:06.59 CS.reconfigure CSconfig @18:18:06.62 CS.hold @18:18:06.68 CS.configure CSconfig @18:18:07.10 PC.reconfigure PCconfig @18:18:07.14 PC.hold @18:18:07.21 PC.configure PCconfig @18:18:07.29 PS.configure PSconfig @18:18:07.40 Forearm.reconfigure LooseForearm @18:18:07.43 Forearm.hold @18:18:07.48 Forearm.configure LooseForearm @18:18:07.56 Elbow.reconfigure LooseElbow @18:18:07.60 Elbow.hold @18:18:07.65 Elbow.configure LooseElbow @18:18:07.75 SC.reconfigure SCloose @18:18:07.79 SC.hold @18:18:07.84 SC.configure SCloose @18:18:07.92 SE.reconfigure SEconfig @18:18:07.96 SE.hold @18:18:08.01 SE.configure SEconfig @18:18:08.05 CH.coast @18:18:08.10 CH.configure CHconfig @18:18:08.12 PH.coast @18:18:08.17 PH.configure PHconfig @18:18:08.21 PCR.off @18:18:08.24 PCR.pidtemp= 50,0.1,0,300,25,25 @18:18:08.27 PCR.caltemp= 2.8814,0.0372,0 @18:18:08.30 All dwarf controllers running firmware version 3.75 @18:18:08.43 Loading /home/esp/esp2/mission/190516totalsampletest.rb mission @18:18:08.49 GoodNews.email "Starting 190516totalsampletest w/pucks in tube 2\n{}\nCan@18:18:04, 20.8C, 24% humidity, 14.7psia, 11.982V, 0.708A, 0.701A avg, 8.48W",Subject: "190516totalsampletest begun" @18:18:08.51
Preparing to sleep until +0:00:15 @18:18:08.58 AS.coast @18:18:08.60
Asleep til +0:00:15 @18:18:08.64 Continuing at 18:18 @18:18:08.65 SS.coast @18:18:08.73 CC.coast @18:18:08.76 CS.coast @18:18:08.79 PC.coast @18:18:08.83 Forearm.coast @18:18:08.86 Elbow.coast @18:18:08.89 SC.coast @18:18:08.92 SE.coast @18:18:09.30 Gate.power :core,:OFF @18:18:09.33 Gate.power :raw,:OFF @18:18:09.34 Shutdown @18:18:15.36 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/190516totalsampletest.rb:111\n{}\nCan@18:18:08, 20.8C, 24% humidity, 14.7psia, 11.987V, 0.701A, 0.701A avg, 8.41W",Subject: "Asleep until 18:18" @18:18:23.46
Powering core bus @18:18:28.14 Gate.power :raw,:ON @18:18:28.75 SPE.coast @18:18:28.80 SPE.configure SPEconfig @18:18:28.91 AS.configure ASconfig @18:18:28.99 SS.configure SSconfig @18:18:29.08 CC.configure CCconfig @18:18:29.17 CS.configure CSconfig @18:18:29.25 PC.configure PCconfig @18:18:29.34 PS.configure PSconfig @18:18:29.44 Forearm.configure LooseForearm @18:18:29.52 Elbow.configure LooseElbow @18:18:29.63 SC.configure SCloose @18:18:29.72 SE.configure SEconfig @18:18:29.77 CH.coast @18:18:29.82 CH.configure CHconfig @18:18:29.85 PH.coast @18:18:29.90 PH.configure PHconfig @18:18:29.93 PCR.off @18:18:29.96 PCR.pidtemp= 50,0.1,0,300,25,25 @18:18:29.99 PCR.caltemp= 2.8814,0.0372,0 @18:18:30.02 All dwarf controllers running firmware version 3.75 @18:18:30.13 Valve.reset @18:18:30.30 PCR.off @18:18:30.33 PCR.pidtemp= 50,0.1,0,300,25,25 @18:18:30.36 PCR.caltemp= 2.8814,0.0372,0 @18:18:30.48 Intake.select :close @18:18:30.54 Exhaust.select :close @18:18:30.56 CTV.dial :air @18:18:30.68 PSV.dial :air @18:18:30.72 CSV.dial :waste1 @18:18:30.81
Forearm.reconfigure LooseForearm @18:18:30.85 Forearm.home.seek :retract @18:18:30.94 SSV.dial :spit,avoiding: :exhaust @18:18:30.96 PS.reconfigure PSconfig @18:18:31.42 PS.home.seek 0ml @18:18:31.51 CS.reconfigure CSfast @18:18:31.55 SS.reconfigure SSconfig @18:18:31.62 CS.home.seek 0ml @18:18:31.66 SS.home.seek 0ml @18:18:31.73 Hand.close @18:18:31.77
Elbow.jog 1500 @18:18:32.30 Elbow.reconfigure LooseElbow @18:18:32.34 Elbow.home.seek :clear @18:18:37.03 SE.reconfigure SEconfig @18:18:37.07 SE.home.seek :down @18:18:37.80 SC.reconfigure SCsloppy @18:18:37.84 SC.home.seek :initial @18:18:40.63 PS.coast @18:18:48.07
Forearm.seek :retract @18:18:48.14 Elbow.seek :hammer @18:18:49.30 Forearm.reconfigure TightForearm @18:18:49.35 Forearm.seek 5 + 350 counts @18:18:50.42 Forearm.seek :retract @18:18:50.79 Session Begun @18:18:51.61 PC.reconfigure PCconfig @18:18:51.64
CC.reconfigure CCconfig @18:18:51.72 PC.home.seek :open @18:18:51.79
CC.home.seek :open @18:18:55.01 Phase [mbaPhase] @18:18:55.02 GoodNews.email "At 18:18:54 began in tube 2: mbaPhase\n/home/esp/esp2/mission/190516totalsampletest.rb:111\n{}\nCan@18:18:54, 20.8C, 25% humidity, 14.7psia, 12.006V, 0.619A, 0.746A avg, 7.44W",Subject: "Started 190516totalsampletest phase mbaPhase" @18:18:55.07CEST16-May-19
==================> STARTED MBAsuperphase (phase #1) <=== @18:18:55.69 Readying the MFB @18:18:55.70 Homing MFB Syringe @18:18:55.71 AFV.select :holdingCoil @18:18:55.89 ATV.dial :air2 @18:18:57.15 AS.reconfigure ASconfig @18:18:57.21 AS.home.seek :home @18:19:02.77 AFV.select :carrierSolution @18:19:02.89 delay 2 seconds @18:19:04.92 AS.seek 1ml @18:19:57.83 delay 1 second @18:19:58.85 AFV.select :holdingCoil @18:19:58.95 delay 1 second @18:19:59.97 ATV.dial :waste @18:20:01.22 AS.reconfigure ASnorm @18:20:01.28 AS.home.seek :empty @18:20:53.87 ATV.dial :waste @18:20:53.89 ARV.dial between carrier and air1 @18:20:54.21 ASV.dial :waste1 @18:20:54.37 Starting mfb @18:20:54.38 pcrBAC sampling at most 1500ml, wcr at most 1500ml @18:20:54.41 BACfans.email "Started mfb in tube 2 at 18:20:54CEST16-May-19\n{}\nCan@18:18:54, 20.8C, 25% humidity, 14.7psia, 12.006V, 0.619A, 0.746A avg, 7.44W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @18:20:54.48
Loading sh1 puck from tube 2 into Collection Clamp @18:20:54.61 Hand.open @18:20:55.59 SC.reconfigure SCloose @18:20:55.75 Elbow.reconfigure TightElbow @18:20:55.82 Elbow.seek 2 @18:20:56.36 Forearm.seek 2 + 700 counts @18:20:57.39 SE.reconfigure SEpick @18:20:57.48 SE.seek beamBreak @18:21:00.56 SE.configure SEconfig @18:21:00.74 Elbow.seek 2 @18:21:00.93 Forearm.seek 2 + 300 counts @18:21:01.17 Forearm.reconfigure LooseForearm @18:21:01.23 Hand.close @18:21:02.00 SE.seek :down @18:21:02.23
Forearm.seek :retract @18:21:03.19 Elbow.seek CC @18:21:04.70 SE.coast @18:21:05.20
Elbow.reconfigure LooseElbow @18:21:05.32 Forearm.reconfigure TightForearm @18:21:05.38 Forearm.seek CC @18:21:06.74 CC.seek :closed @18:21:10.99 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.46 @18:21:11.00 Hand.open @18:21:11.81 Forearm.reconfigure LooseForearm @18:21:11.87 Forearm.seek :retract @18:21:12.92 SC.coast @18:21:12.97 collectSample 1500ml w/bubble point of 55psi @18:21:12.98 Priming 7ml @18:21:13.00 Gate.power :sampler,:ON @18:21:13.03 Intake.select :close @18:21:13.05 Exhaust.select :close @18:21:13.08 SS.hold @18:21:13.30 Calibrating pressure and temperature sensors @18:21:13.32 CTV.dial :air @18:21:13.35 SSV.dial :spit,avoiding: :exhaust @18:21:13.38 SS.reconfigure SSfast @18:21:13.45 SS.seek 5ml @18:21:16.54 SSV.dial CBV @18:21:16.57
CBV.dial SSV @18:21:18.08 delay 3.8 seconds @18:21:21.99 SSV.dial :spit @18:21:23.50 SS.seek 0ml @18:21:26.35 SSV.dial CBV @18:21:27.83 CTV.dial :intake @18:21:28.70 Waiting for external sampler to finish priming... @18:21:28.71 delayUntil "18:26:13.02" @18:26:13.04 SSV.dial CBV @18:26:13.07
CBV.dial SSV @18:26:13.10 Allowing 40s for 7.0ml of remaining 7.0ml @18:26:13.12 Intake.select :open @18:26:15.30 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:26:15.32 SS.reconfigure SSinhale.with maxSpeed: 150 @18:26:15.39 SS.seek 7ml @18:26:20.84 Stroke completed -- waiting for pressure to equalize ... @18:26:34.89 Intake.select :close @18:26:35.04 SSV.dial :exhaust @18:26:35.91 Exhaust.select :open @18:26:36.04 SS.reconfigure SSexhale @18:26:36.10 SS.seek 0ml @18:26:40.06 Exhaust.select :close @18:26:40.19 Sampling 1500.0ml @18:26:40.21 Intake.select :close @18:26:40.23 Exhaust.select :close @18:26:40.26 SS.reconfigure SSconfig @18:26:40.33 SS.hold @18:26:40.53 CTV.dial :intake @18:26:40.97 SSV.dial CBV @18:26:41.01
CBV.dial SSV @18:26:41.91 Allowing 130s for 25.0ml of remaining 1500.0ml @18:26:41.92 Intake.select :open @18:26:44.12 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:26:44.14 SS.reconfigure SSinhale.with maxSpeed: 150 @18:26:44.21 SS.seek 25ml @18:26:48.76 Vacuum too high -- waiting up to 40s @18:26:55.06 Intake @15.3psia, vacuum from 8.8 to 12.8psi @18:26:55.07 SS.reconfigure SSinhale.with maxSpeed: 135 @18:26:55.15 SS.seek 25ml @18:26:58.51 Vacuum too high -- waiting up to 40s @18:27:05.23 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:27:05.25 SS.reconfigure SSinhale.with maxSpeed: 122 @18:27:05.32 SS.seek 25ml @18:27:08.83 Vacuum too high -- waiting up to 40s @18:27:14.65 Intake @15.3psia, vacuum from 8.8 to 12.8psi @18:27:14.67 SS.reconfigure SSinhale.with maxSpeed: 110 @18:27:14.74 SS.seek 25ml @18:27:18.48 Vacuum too high -- waiting up to 40s @18:27:24.78 Intake @15.4psia, vacuum from 8.9 to 12.9psi @18:27:24.79 SS.reconfigure SSinhale.with maxSpeed: 99 @18:27:24.87 SS.seek 25ml @18:27:29.24 Vacuum too high -- waiting up to 40s @18:27:35.96 Intake @15.3psia, vacuum from 8.8 to 12.8psi @18:27:35.97 SS.reconfigure SSinhale.with maxSpeed: 89 @18:27:36.05 SS.seek 25ml @18:27:39.47 Stroke completed -- waiting for pressure to equalize ... @18:27:45.56 Intake.select :close @18:27:45.71 SSV.dial :exhaust @18:27:46.60 Exhaust.select :open @18:27:46.72 SS.reconfigure SSexhale @18:27:46.78 SS.seek 0ml @18:27:56.27 Exhaust.select :close @18:27:56.39 SSV.dial CBV @18:27:56.42
CBV.dial SSV @18:27:57.32 Allowing 130s for 25.0ml of remaining 1475.0ml @18:27:57.33 Intake.select :open @18:27:59.51 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:27:59.53 SS.reconfigure SSinhale.with maxSpeed: 89 @18:27:59.60 SS.seek 25ml @18:28:03.48 Vacuum too high -- waiting up to 40s @18:28:07.66 Intake @15.3psia, vacuum from 8.8 to 12.8psi @18:28:07.68 SS.reconfigure SSinhale.with maxSpeed: 80 @18:28:07.76 SS.seek 25ml @18:28:11.76 Vacuum too high -- waiting up to 40s @18:28:16.42 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:28:16.44 SS.reconfigure SSinhale.with maxSpeed: 72 @18:28:16.52 SS.seek 25ml @18:28:20.95 Vacuum too high -- waiting up to 40s @18:28:25.60 Intake @15.4psia, vacuum from 8.9 to 12.9psi @18:28:25.62 SS.reconfigure SSinhale.with maxSpeed: 65 @18:28:25.70 SS.seek 25ml @18:28:30.54 Vacuum too high -- waiting up to 40s @18:28:35.18 Intake @15.4psia, vacuum from 8.9 to 12.9psi @18:28:35.19 SS.reconfigure SSinhale.with maxSpeed: 59 @18:28:35.27 SS.seek 25ml @18:28:41.04 Vacuum too high -- waiting up to 40s @18:28:45.48 Intake @15.4psia, vacuum from 8.9 to 12.9psi @18:28:45.50 SS.reconfigure SSinhale.with maxSpeed: 53 @18:28:45.57 SS.seek 25ml @18:28:52.23 Vacuum too high -- waiting up to 40s @18:28:56.89 Intake @15.3psia, vacuum from 8.8 to 12.8psi @18:28:56.91 SS.reconfigure SSinhale.with maxSpeed: 48 @18:28:56.98 SS.seek 25ml @18:28:58.76 Stroke completed -- waiting for pressure to equalize ... @18:29:01.24 Intake.select :close @18:29:01.40 SSV.dial :exhaust @18:29:02.28 Exhaust.select :open @18:29:02.41 SS.reconfigure SSexhale @18:29:02.47 SS.seek 0ml @18:29:11.91 Exhaust.select :close @18:29:12.03 SSV.dial CBV @18:29:12.06
CBV.dial SSV @18:29:12.94 Allowing 130s for 25.0ml of remaining 1450.0ml @18:29:12.95 Intake.select :open @18:29:15.14 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:29:15.15 SS.reconfigure SSinhale.with maxSpeed: 48 @18:29:15.23 SS.seek 25ml @18:29:23.54 Vacuum too high -- waiting up to 40s @18:29:27.02 Intake @15.3psia, vacuum from 8.8 to 12.8psi @18:29:27.04 SS.reconfigure SSinhale.with maxSpeed: 43 @18:29:27.11 SS.seek 25ml @18:29:37.73 Vacuum too high -- waiting up to 40s @18:29:41.93 Intake @15.4psia, vacuum from 8.9 to 12.9psi @18:29:41.94 SS.reconfigure SSinhale.with maxSpeed: 39 @18:29:42.02 SS.seek 25ml @18:30:00.48 Vacuum too high -- waiting up to 40s @18:30:04.91 SS.reconfigure SSinhale.with maxSpeed: 35 @18:30:04.98 SS.seek 25ml @18:30:12.18 Stroke completed -- waiting for pressure to equalize ... @18:30:16.31 Intake.select :close @18:30:16.47 SSV.dial :exhaust @18:30:17.36 Exhaust.select :open @18:30:17.49 SS.reconfigure SSexhale @18:30:17.55 SS.seek 0ml @18:30:26.76 Exhaust.select :close @18:30:26.89 SSV.dial CBV @18:30:26.92
CBV.dial SSV @18:30:27.83 Allowing 130s for 25.0ml of remaining 1425.0ml @18:30:27.84 Intake.select :open @18:30:30.04 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:30:30.06 SS.reconfigure SSinhale.with maxSpeed: 35 @18:30:30.13 SS.seek 25ml @18:31:21.13 Stroke completed -- waiting for pressure to equalize ... @18:31:28.44 Intake.select :close @18:31:28.60 SSV.dial :exhaust @18:31:29.49 Exhaust.select :open @18:31:29.62 SS.reconfigure SSexhale @18:31:29.68 SS.seek 0ml @18:31:39.16 Exhaust.select :close @18:31:39.29 SSV.dial CBV @18:31:39.32
CBV.dial SSV @18:31:40.22 Allowing 130s for 25.0ml of remaining 1400.0ml @18:31:40.23 Intake.select :open @18:31:42.42 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:31:42.43 SS.reconfigure SSinhale.with maxSpeed: 35 @18:31:42.51 SS.seek 25ml @18:32:33.01 Stroke completed -- waiting for pressure to equalize ... @18:32:39.74 Intake.select :close @18:32:39.90 SSV.dial :exhaust @18:32:40.77 Exhaust.select :open @18:32:40.90 SS.reconfigure SSexhale @18:32:40.96 SS.seek 0ml @18:32:50.66 Exhaust.select :close @18:32:50.78 SSV.dial CBV @18:32:50.81
CBV.dial SSV @18:32:51.70 Allowing 130s for 25.0ml of remaining 1375.0ml @18:32:51.71 Intake.select :open @18:32:53.90 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:32:53.91 SS.reconfigure SSinhale.with maxSpeed: 35 @18:32:53.99 SS.seek 25ml @18:33:44.32 Stroke completed -- waiting for pressure to equalize ... @18:33:50.42 Intake.select :close @18:33:50.58 SSV.dial :exhaust @18:33:51.45 Exhaust.select :open @18:33:51.58 SS.reconfigure SSexhale @18:33:51.64 SS.seek 0ml @18:34:01.30 Exhaust.select :close @18:34:01.85 SSV.dial CBV @18:34:01.88
CBV.dial SSV @18:34:02.78 Allowing 130s for 25.0ml of remaining 1350.0ml @18:34:02.79 Intake.select :open @18:34:04.98 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:34:05.00 SS.reconfigure SSinhale.with maxSpeed: 35 @18:34:05.07 SS.seek 25ml @18:34:55.49 Stroke completed -- waiting for pressure to equalize ... @18:35:01.63 Intake.select :close @18:35:01.78 SSV.dial :exhaust @18:35:02.66 Exhaust.select :open @18:35:02.79 SS.reconfigure SSexhale @18:35:02.85 SS.seek 0ml @18:35:12.71 Exhaust.select :close @18:35:12.84 SSV.dial CBV @18:35:12.87
CBV.dial SSV @18:35:13.78 Allowing 130s for 25.0ml of remaining 1325.0ml @18:35:13.79 Intake.select :open @18:35:15.97 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:35:15.99 SS.reconfigure SSinhale.with maxSpeed: 35 @18:35:16.06 SS.seek 25ml @18:36:06.38 Stroke completed -- waiting for pressure to equalize ... @18:36:11.87 Intake.select :close @18:36:12.03 SSV.dial :exhaust @18:36:12.91 Exhaust.select :open @18:36:13.04 SS.reconfigure SSexhale @18:36:13.10 SS.seek 0ml @18:36:22.82 Exhaust.select :close @18:36:22.94 SSV.dial CBV @18:36:22.97
CBV.dial SSV @18:36:23.88 Allowing 130s for 25.0ml of remaining 1300.0ml @18:36:23.89 Intake.select :open @18:36:26.08 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:36:26.09 SS.reconfigure SSinhale.with maxSpeed: 35 @18:36:26.17 SS.seek 25ml @18:37:16.90 Stroke completed -- waiting for pressure to equalize ... @18:37:23.02 Intake.select :close @18:37:23.18 SSV.dial :exhaust @18:37:24.06 Exhaust.select :open @18:37:24.19 SS.reconfigure SSexhale @18:37:24.25 SS.seek 0ml @18:37:33.49 Exhaust.select :close @18:37:33.61 SSV.dial CBV @18:37:33.64
CBV.dial SSV @18:37:34.52 Allowing 130s for 25.0ml of remaining 1275.0ml @18:37:34.53 Intake.select :open @18:37:36.71 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:37:36.73 SS.reconfigure SSinhale.with maxSpeed: 35 @18:37:36.80 SS.seek 25ml @18:38:27.96 Stroke completed -- waiting for pressure to equalize ... @18:38:33.89 Intake.select :close @18:38:34.03 SSV.dial :exhaust @18:38:34.91 Exhaust.select :open @18:38:35.04 SS.reconfigure SSexhale @18:38:35.10 SS.seek 0ml @18:38:44.36 Exhaust.select :close @18:38:44.48 SSV.dial CBV @18:38:44.51
CBV.dial SSV @18:38:45.41 Allowing 130s for 25.0ml of remaining 1250.0ml @18:38:45.42 Intake.select :open @18:38:47.61 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:38:47.63 SS.reconfigure SSinhale.with maxSpeed: 35 @18:38:47.70 SS.seek 25ml @18:39:38.40 Stroke completed -- waiting for pressure to equalize ... @18:39:43.90 Intake.select :close @18:39:44.05 SSV.dial :exhaust @18:39:44.94 Exhaust.select :open @18:39:45.07 SS.reconfigure SSexhale @18:39:45.13 SS.seek 0ml @18:39:54.77 Exhaust.select :close @18:39:54.90 SSV.dial CBV @18:39:54.93
CBV.dial SSV @18:39:55.81 Allowing 130s for 25.0ml of remaining 1225.0ml @18:39:55.82 Intake.select :open @18:39:58.00 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:39:58.02 SS.reconfigure SSinhale.with maxSpeed: 35 @18:39:58.09 SS.seek 25ml @18:40:48.52 Stroke completed -- waiting for pressure to equalize ... @18:40:53.43 Intake.select :close @18:40:53.60 SSV.dial :exhaust @18:40:54.47 Exhaust.select :open @18:40:54.60 SS.reconfigure SSexhale @18:40:54.66 SS.seek 0ml @18:41:04.43 Exhaust.select :close @18:41:04.56 SSV.dial CBV @18:41:04.59
CBV.dial SSV @18:41:05.47 Allowing 130s for 25.0ml of remaining 1200.0ml @18:41:05.48 Intake.select :open @18:41:07.66 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:41:07.68 SS.reconfigure SSinhale.with maxSpeed: 35 @18:41:07.75 SS.seek 25ml @18:41:58.19 Stroke completed -- waiting for pressure to equalize ... @18:42:03.07 Intake.select :close @18:42:03.24 SSV.dial :exhaust @18:42:04.11 Exhaust.select :open @18:42:04.24 SS.reconfigure SSexhale @18:42:04.30 SS.seek 0ml @18:42:13.80 Exhaust.select :close @18:42:13.93 SSV.dial CBV @18:42:13.96
CBV.dial SSV @18:42:14.84 Allowing 130s for 25.0ml of remaining 1175.0ml @18:42:14.85 Intake.select :open @18:42:17.03 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:42:17.05 SS.reconfigure SSinhale.with maxSpeed: 35 @18:42:17.12 SS.seek 25ml @18:43:07.77 Stroke completed -- waiting for pressure to equalize ... @18:43:12.49 Intake.select :close @18:43:12.65 SSV.dial :exhaust @18:43:13.51 Exhaust.select :open @18:43:13.63 SS.reconfigure SSexhale @18:43:13.69 SS.seek 0ml @18:43:23.21 Exhaust.select :close @18:43:23.34 SSV.dial CBV @18:43:23.37
CBV.dial SSV @18:43:24.25 Allowing 130s for 25.0ml of remaining 1150.0ml @18:43:24.26 Intake.select :open @18:43:26.45 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:43:26.47 SS.reconfigure SSinhale.with maxSpeed: 35 @18:43:26.54 SS.seek 25ml @18:44:17.21 Stroke completed -- waiting for pressure to equalize ... @18:44:22.11 Intake.select :close @18:44:22.27 SSV.dial :exhaust @18:44:23.14 Exhaust.select :open @18:44:23.26 SS.reconfigure SSexhale @18:44:23.32 SS.seek 0ml @18:44:33.05 Exhaust.select :close @18:44:33.18 SSV.dial CBV @18:44:33.21
CBV.dial SSV @18:44:34.09 Allowing 130s for 25.0ml of remaining 1125.0ml @18:44:34.10 Intake.select :open @18:44:36.29 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:44:36.30 SS.reconfigure SSinhale.with maxSpeed: 35 @18:44:36.38 SS.seek 25ml @18:45:26.80 Stroke completed -- waiting for pressure to equalize ... @18:45:31.68 Intake.select :close @18:45:31.84 SSV.dial :exhaust @18:45:32.72 Exhaust.select :open @18:45:32.85 SS.reconfigure SSexhale @18:45:32.91 SS.seek 0ml @18:45:42.50 Exhaust.select :close @18:45:42.62 SSV.dial CBV @18:45:42.65
CBV.dial SSV @18:45:43.53 Allowing 130s for 25.0ml of remaining 1100.0ml @18:45:43.54 Intake.select :open @18:45:45.72 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:45:45.74 SS.reconfigure SSinhale.with maxSpeed: 35 @18:45:45.81 SS.seek 25ml @18:46:36.25 Stroke completed -- waiting for pressure to equalize ... @18:46:41.15 Intake.select :close @18:46:41.31 SSV.dial :exhaust @18:46:42.17 Exhaust.select :open @18:46:42.30 SS.reconfigure SSexhale @18:46:42.36 SS.seek 0ml @18:46:51.94 Exhaust.select :close @18:46:52.07 SSV.dial CBV @18:46:52.10
CBV.dial SSV @18:46:52.98 Allowing 130s for 25.0ml of remaining 1075.0ml @18:46:52.99 Intake.select :open @18:46:55.18 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:46:55.20 SS.reconfigure SSinhale.with maxSpeed: 35 @18:46:55.27 SS.seek 25ml @18:47:45.83 Stroke completed -- waiting for pressure to equalize ... @18:47:50.56 Intake.select :close @18:47:50.72 SSV.dial :exhaust @18:47:51.61 Exhaust.select :open @18:47:51.73 SS.reconfigure SSexhale @18:47:51.79 SS.seek 0ml @18:48:01.45 Exhaust.select :close @18:48:01.57 SSV.dial CBV @18:48:01.60
CBV.dial SSV @18:48:02.50 Allowing 130s for 25.0ml of remaining 1050.0ml @18:48:02.51 Intake.select :open @18:48:04.69 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:48:04.71 SS.reconfigure SSinhale.with maxSpeed: 35 @18:48:04.79 SS.seek 25ml @18:48:55.22 Stroke completed -- waiting for pressure to equalize ... @18:48:59.50 Intake.select :close @18:48:59.66 SSV.dial :exhaust @18:49:00.55 Exhaust.select :open @18:49:00.68 SS.reconfigure SSexhale @18:49:00.74 SS.seek 0ml @18:49:10.44 Exhaust.select :close @18:49:10.57 SSV.dial CBV @18:49:10.60
CBV.dial SSV @18:49:11.48 Allowing 130s for 25.0ml of remaining 1025.0ml @18:49:11.49 Intake.select :open @18:49:13.67 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:49:13.69 SS.reconfigure SSinhale.with maxSpeed: 35 @18:49:13.77 SS.seek 25ml @18:50:04.16 Stroke completed -- waiting for pressure to equalize ... @18:50:08.45 Intake.select :close @18:50:08.60 SSV.dial :exhaust @18:50:09.49 Exhaust.select :open @18:50:09.62 SS.reconfigure SSexhale @18:50:09.68 SS.seek 0ml @18:50:18.88 Exhaust.select :close @18:50:19.01 SSV.dial CBV @18:50:19.04
CBV.dial SSV @18:50:19.92 Allowing 130s for 25.0ml of remaining 1000.0ml @18:50:19.93 Intake.select :open @18:50:22.12 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:50:22.13 SS.reconfigure SSinhale.with maxSpeed: 35 @18:50:22.21 SS.seek 25ml @18:51:13.44 Stroke completed -- waiting for pressure to equalize ... @18:51:18.31 Intake.select :close @18:51:18.48 SSV.dial :exhaust @18:51:19.34 Exhaust.select :open @18:51:19.47 SS.reconfigure SSexhale @18:51:19.53 SS.seek 0ml @18:51:29.19 Exhaust.select :close @18:51:29.32 SSV.dial CBV @18:51:29.35
CBV.dial SSV @18:51:30.65 Allowing 130s for 25.0ml of remaining 975.0ml @18:51:30.66 Intake.select :open @18:51:32.85 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:51:32.86 SS.reconfigure SSinhale.with maxSpeed: 35 @18:51:32.94 SS.seek 25ml @18:52:23.36 Stroke completed -- waiting for pressure to equalize ... @18:52:27.68 Intake.select :close @18:52:27.83 SSV.dial :exhaust @18:52:28.74 Exhaust.select :open @18:52:28.88 SS.reconfigure SSexhale @18:52:28.94 SS.seek 0ml @18:52:38.44 Exhaust.select :close @18:52:38.57 SSV.dial CBV @18:52:38.60
CBV.dial SSV @18:52:39.48 Allowing 130s for 25.0ml of remaining 950.0ml @18:52:39.49 Intake.select :open @18:52:41.67 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:52:41.69 SS.reconfigure SSinhale.with maxSpeed: 35 @18:52:41.76 SS.seek 25ml @18:53:32.42 Stroke completed -- waiting for pressure to equalize ... @18:53:37.33 Intake.select :close @18:53:37.50 SSV.dial :exhaust @18:53:38.39 Exhaust.select :open @18:53:38.52 SS.reconfigure SSexhale @18:53:38.58 SS.seek 0ml @18:53:48.16 Exhaust.select :close @18:53:48.28 SSV.dial CBV @18:53:48.31
CBV.dial SSV @18:53:49.20 Allowing 130s for 25.0ml of remaining 925.0ml @18:53:49.21 Intake.select :open @18:53:51.40 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:53:51.41 SS.reconfigure SSinhale.with maxSpeed: 35 @18:53:51.49 SS.seek 25ml @18:54:42.05 Stroke completed -- waiting for pressure to equalize ... @18:54:46.32 Intake.select :close @18:54:46.46 SSV.dial :exhaust @18:54:47.32 Exhaust.select :open @18:54:47.45 SS.reconfigure SSexhale @18:54:47.51 SS.seek 0ml @18:54:56.73 Exhaust.select :close @18:54:56.85 SSV.dial CBV @18:54:56.88
CBV.dial SSV @18:54:57.76 Allowing 130s for 25.0ml of remaining 900.0ml @18:54:57.77 Intake.select :open @18:54:59.95 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:54:59.97 SS.reconfigure SSinhale.with maxSpeed: 35 @18:55:00.04 SS.seek 25ml @18:55:51.19 Stroke completed -- waiting for pressure to equalize ... @18:55:56.52 Intake.select :close @18:55:56.67 SSV.dial :exhaust @18:55:57.56 Exhaust.select :open @18:55:57.69 SS.reconfigure SSexhale @18:55:57.75 SS.seek 0ml @18:56:07.34 Exhaust.select :close @18:56:07.46 SSV.dial CBV @18:56:07.49
CBV.dial SSV @18:56:08.39 Allowing 130s for 25.0ml of remaining 875.0ml @18:56:08.40 Intake.select :open @18:56:10.59 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:56:10.61 SS.reconfigure SSinhale.with maxSpeed: 35 @18:56:10.69 SS.seek 25ml @18:57:01.47 Stroke completed -- waiting for pressure to equalize ... @18:57:06.37 Intake.select :close @18:57:06.56 SSV.dial :exhaust @18:57:07.45 Exhaust.select :open @18:57:07.58 SS.reconfigure SSexhale @18:57:07.64 SS.seek 0ml @18:57:17.20 Exhaust.select :close @18:57:17.33 SSV.dial CBV @18:57:17.36
CBV.dial SSV @18:57:18.24 Allowing 130s for 25.0ml of remaining 850.0ml @18:57:18.25 Intake.select :open @18:57:20.43 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:57:20.45 SS.reconfigure SSinhale.with maxSpeed: 35 @18:57:20.52 SS.seek 25ml @18:58:11.01 Stroke completed -- waiting for pressure to equalize ... @18:58:15.32 Intake.select :close @18:58:15.47 SSV.dial :exhaust @18:58:16.34 Exhaust.select :open @18:58:16.47 SS.reconfigure SSexhale @18:58:16.53 SS.seek 0ml @18:58:26.08 Exhaust.select :close @18:58:26.21 SSV.dial CBV @18:58:26.24
CBV.dial SSV @18:58:27.12 Allowing 130s for 25.0ml of remaining 825.0ml @18:58:27.13 Intake.select :open @18:58:29.31 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:58:29.33 SS.reconfigure SSinhale.with maxSpeed: 35 @18:58:29.40 SS.seek 25ml @18:59:20.00 Stroke completed -- waiting for pressure to equalize ... @18:59:24.30 Intake.select :close @18:59:24.44 SSV.dial :exhaust @18:59:25.32 Exhaust.select :open @18:59:25.45 SS.reconfigure SSexhale @18:59:25.51 SS.seek 0ml @18:59:35.03 Exhaust.select :close @18:59:35.15 SSV.dial CBV @18:59:35.18
CBV.dial SSV @18:59:36.07 Allowing 130s for 25.0ml of remaining 800.0ml @18:59:36.08 Intake.select :open @18:59:38.26 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:59:38.28 SS.reconfigure SSinhale.with maxSpeed: 35 @18:59:38.35 SS.seek 25ml @19:00:28.98 Stroke completed -- waiting for pressure to equalize ... @19:00:33.72 Intake.select :close @19:00:33.88 SSV.dial :exhaust @19:00:34.74 Exhaust.select :open @19:00:34.87 SS.reconfigure SSexhale @19:00:34.93 SS.seek 0ml @19:00:44.65 Exhaust.select :close @19:00:44.77 SSV.dial CBV @19:00:44.80
CBV.dial SSV @19:00:45.68 Allowing 130s for 25.0ml of remaining 775.0ml @19:00:45.69 Intake.select :open @19:00:47.88 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:00:47.90 SS.reconfigure SSinhale.with maxSpeed: 35 @19:00:47.97 SS.seek 25ml @19:01:38.42 Stroke completed -- waiting for pressure to equalize ... @19:01:42.72 Intake.select :close @19:01:42.88 SSV.dial :exhaust @19:01:43.78 Exhaust.select :open @19:01:43.91 SS.reconfigure SSexhale @19:01:43.97 SS.seek 0ml @19:01:53.48 Exhaust.select :close @19:01:53.61 SSV.dial CBV @19:01:53.64
CBV.dial SSV @19:01:54.52 Allowing 130s for 25.0ml of remaining 750.0ml @19:01:54.53 Intake.select :open @19:01:56.73 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:01:56.74 SS.reconfigure SSinhale.with maxSpeed: 35 @19:01:56.82 SS.seek 25ml @19:02:47.42 Stroke completed -- waiting for pressure to equalize ... @19:02:51.69 Intake.select :close @19:02:51.84 SSV.dial :exhaust @19:02:52.74 Exhaust.select :open @19:02:52.87 SS.reconfigure SSexhale @19:02:52.93 SS.seek 0ml @19:03:02.86 Exhaust.select :close @19:03:02.99 SSV.dial CBV @19:03:03.02
CBV.dial SSV @19:03:03.91 Allowing 130s for 25.0ml of remaining 725.0ml @19:03:03.92 Intake.select :open @19:03:06.10 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:03:06.12 SS.reconfigure SSinhale.with maxSpeed: 35 @19:03:06.19 SS.seek 25ml @19:03:56.34 Stroke completed -- waiting for pressure to equalize ... @19:04:00.03 Intake.select :close @19:04:00.17 SSV.dial :exhaust @19:04:01.05 Exhaust.select :open @19:04:01.18 SS.reconfigure SSexhale @19:04:01.24 SS.seek 0ml @19:04:11.00 Exhaust.select :close @19:04:11.12 SSV.dial CBV @19:04:11.49
CBV.dial SSV @19:04:12.45 Allowing 130s for 25.0ml of remaining 700.0ml @19:04:12.46 Intake.select :open @19:04:14.66 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:04:14.68 SS.reconfigure SSinhale.with maxSpeed: 35 @19:04:14.75 SS.seek 25ml @19:05:05.37 Stroke completed -- waiting for pressure to equalize ... @19:05:09.09 Intake.select :close @19:05:09.25 SSV.dial :exhaust @19:05:10.12 Exhaust.select :open @19:05:10.25 SS.reconfigure SSexhale @19:05:10.31 SS.seek 0ml @19:05:20.01 Exhaust.select :close @19:05:20.14 SSV.dial CBV @19:05:20.17
CBV.dial SSV @19:05:21.05 Allowing 130s for 25.0ml of remaining 675.0ml @19:05:21.06 Intake.select :open @19:05:23.24 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:05:23.26 SS.reconfigure SSinhale.with maxSpeed: 35 @19:05:23.33 SS.seek 25ml @19:06:13.67 Stroke completed -- waiting for pressure to equalize ... @19:06:17.95 Intake.select :close @19:06:18.11 SSV.dial :exhaust @19:06:19.00 Exhaust.select :open @19:06:19.13 SS.reconfigure SSexhale @19:06:19.19 SS.seek 0ml @19:06:28.93 Exhaust.select :close @19:06:29.06 SSV.dial CBV @19:06:29.09
CBV.dial SSV @19:06:29.98 Allowing 130s for 25.0ml of remaining 650.0ml @19:06:29.99 Intake.select :open @19:06:32.18 Intake @15.6psia, vacuum from 9.1 to 13.1psi @19:06:32.19 SS.reconfigure SSinhale.with maxSpeed: 35 @19:06:32.27 SS.seek 25ml @19:07:23.42 Stroke completed -- waiting for pressure to equalize ... @19:07:27.73 Intake.select :close @19:07:27.88 SSV.dial :exhaust @19:07:28.77 Exhaust.select :open @19:07:28.90 SS.reconfigure SSexhale @19:07:28.96 SS.seek 0ml @19:07:38.45 Exhaust.select :close @19:07:38.57 SSV.dial CBV @19:07:38.60
CBV.dial SSV @19:07:39.49 Allowing 130s for 25.0ml of remaining 625.0ml @19:07:39.50 Intake.select :open @19:07:41.68 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:07:41.70 SS.reconfigure SSinhale.with maxSpeed: 35 @19:07:41.78 SS.seek 25ml @19:08:32.48 Stroke completed -- waiting for pressure to equalize ... @19:08:37.80 Intake.select :close @19:08:37.94 SSV.dial :exhaust @19:08:38.80 Exhaust.select :open @19:08:38.93 SS.reconfigure SSexhale @19:08:38.99 SS.seek 0ml @19:08:48.56 Exhaust.select :close @19:08:48.70 SSV.dial CBV @19:08:48.72
CBV.dial SSV @19:08:49.60 Allowing 130s for 25.0ml of remaining 600.0ml @19:08:49.61 Intake.select :open @19:08:51.80 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:08:51.82 SS.reconfigure SSinhale.with maxSpeed: 35 @19:08:51.89 SS.seek 25ml @19:09:42.48 Stroke completed -- waiting for pressure to equalize ... @19:09:46.78 Intake.select :close @19:09:46.93 SSV.dial :exhaust @19:09:47.83 Exhaust.select :open @19:09:47.96 SS.reconfigure SSexhale @19:09:48.02 SS.seek 0ml @19:09:57.61 Exhaust.select :close @19:09:57.74 SSV.dial CBV @19:09:57.77
CBV.dial SSV @19:09:58.66 Allowing 130s for 25.0ml of remaining 575.0ml @19:09:58.67 Intake.select :open @19:10:00.85 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:10:00.87 SS.reconfigure SSinhale.with maxSpeed: 35 @19:10:00.95 SS.seek 25ml @19:10:51.73 Stroke completed -- waiting for pressure to equalize ... @19:10:56.62 Intake.select :close @19:10:56.78 SSV.dial :exhaust @19:10:57.65 Exhaust.select :open @19:10:57.78 SS.reconfigure SSexhale @19:10:57.84 SS.seek 0ml @19:11:07.04 Exhaust.select :close @19:11:07.16 SSV.dial CBV @19:11:07.19
CBV.dial SSV @19:11:08.08 Allowing 130s for 25.0ml of remaining 550.0ml @19:11:08.09 Intake.select :open @19:11:10.27 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:11:10.29 SS.reconfigure SSinhale.with maxSpeed: 35 @19:11:10.36 SS.seek 25ml @19:12:01.62 Stroke completed -- waiting for pressure to equalize ... @19:12:06.54 Intake.select :close @19:12:06.69 SSV.dial :exhaust @19:12:07.57 Exhaust.select :open @19:12:07.70 SS.reconfigure SSexhale @19:12:07.76 SS.seek 0ml @19:12:17.37 Exhaust.select :close @19:12:17.50 SSV.dial CBV @19:12:17.53
CBV.dial SSV @19:12:18.41 Allowing 130s for 25.0ml of remaining 525.0ml @19:12:18.42 Intake.select :open @19:12:20.60 Intake @15.4psia, vacuum from 8.9 to 12.9psi @19:12:20.62 SS.reconfigure SSinhale.with maxSpeed: 35 @19:12:20.69 SS.seek 25ml @19:13:11.31 Stroke completed -- waiting for pressure to equalize ... @19:13:15.43 Intake.select :close @19:13:15.59 SSV.dial :exhaust @19:13:16.48 Exhaust.select :open @19:13:16.60 SS.reconfigure SSexhale @19:13:16.66 SS.seek 0ml @19:13:26.34 Exhaust.select :close @19:13:26.47 SSV.dial CBV @19:13:26.50
CBV.dial SSV @19:13:27.38 Allowing 130s for 25.0ml of remaining 500.0ml @19:13:27.39 Intake.select :open @19:13:29.58 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:13:29.60 SS.reconfigure SSinhale.with maxSpeed: 35 @19:13:29.68 SS.seek 25ml @19:14:20.35 Stroke completed -- waiting for pressure to equalize ... @19:14:25.26 Intake.select :close @19:14:25.43 SSV.dial :exhaust @19:14:26.30 Exhaust.select :open @19:14:26.43 SS.reconfigure SSexhale @19:14:26.49 SS.seek 0ml @19:14:35.67 Exhaust.select :close @19:14:35.80 SSV.dial CBV @19:14:35.83
CBV.dial SSV @19:14:36.71 Allowing 130s for 25.0ml of remaining 475.0ml @19:14:36.72 Intake.select :open @19:14:38.91 Intake @15.4psia, vacuum from 8.9 to 12.9psi @19:14:38.93 SS.reconfigure SSinhale.with maxSpeed: 35 @19:14:39.00 SS.seek 25ml @19:15:30.31 Stroke completed -- waiting for pressure to equalize ... @19:15:35.18 Intake.select :close @19:15:35.34 SSV.dial :exhaust @19:15:36.21 Exhaust.select :open @19:15:36.34 SS.reconfigure SSexhale @19:15:36.40 SS.seek 0ml @19:15:46.28 Exhaust.select :close @19:15:46.40 SSV.dial CBV @19:15:46.43
CBV.dial SSV @19:15:47.30 Allowing 130s for 25.0ml of remaining 450.0ml @19:15:47.31 Intake.select :open @19:15:49.48 Intake @15.3psia, vacuum from 8.8 to 12.8psi @19:15:49.50 SS.reconfigure SSinhale.with maxSpeed: 35 @19:15:49.58 SS.seek 25ml @19:16:39.76 Stroke completed -- waiting for pressure to equalize ... @19:16:44.08 Intake.select :close @19:16:44.24 SSV.dial :exhaust @19:16:45.12 Exhaust.select :open @19:16:45.25 SS.reconfigure SSexhale @19:16:45.31 SS.seek 0ml @19:16:55.26 Exhaust.select :close @19:16:55.39 SSV.dial CBV @19:16:55.42
CBV.dial SSV @19:16:56.30 Allowing 130s for 25.0ml of remaining 425.0ml @19:16:56.31 Intake.select :open @19:16:58.51 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:16:58.52 SS.reconfigure SSinhale.with maxSpeed: 35 @19:16:58.60 SS.seek 25ml @19:17:49.40 Stroke completed -- waiting for pressure to equalize ... @19:17:53.72 Intake.select :close @19:17:53.87 SSV.dial :exhaust @19:17:54.75 Exhaust.select :open @19:17:54.88 SS.reconfigure SSexhale @19:17:54.94 SS.seek 0ml @19:18:04.48 Exhaust.select :close @19:18:04.61 SSV.dial CBV @19:18:04.64
CBV.dial SSV @19:18:05.50 Allowing 130s for 25.0ml of remaining 400.0ml @19:18:05.51 Intake.select :open @19:18:07.69 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:18:07.71 SS.reconfigure SSinhale.with maxSpeed: 35 @19:18:07.79 SS.seek 25ml @19:18:58.81 Stroke completed -- waiting for pressure to equalize ... @19:19:03.10 Intake.select :close @19:19:03.26 SSV.dial :exhaust @19:19:04.14 Exhaust.select :open @19:19:04.27 SS.reconfigure SSexhale @19:19:04.33 SS.seek 0ml @19:19:13.93 Exhaust.select :close @19:19:14.06 SSV.dial CBV @19:19:14.09
CBV.dial SSV @19:19:14.97 Allowing 130s for 25.0ml of remaining 375.0ml @19:19:14.98 Intake.select :open @19:19:17.17 Intake @15.4psia, vacuum from 8.9 to 12.9psi @19:19:17.19 SS.reconfigure SSinhale.with maxSpeed: 35 @19:19:17.26 SS.seek 25ml @19:20:08.01 Stroke completed -- waiting for pressure to equalize ... @19:20:12.28 Intake.select :close @19:20:12.44 SSV.dial :exhaust @19:20:13.30 Exhaust.select :open @19:20:13.43 SS.reconfigure SSexhale @19:20:13.49 SS.seek 0ml @19:20:22.73 Exhaust.select :close @19:20:22.86 SSV.dial CBV @19:20:22.89
CBV.dial SSV @19:20:23.77 Allowing 130s for 25.0ml of remaining 350.0ml @19:20:23.78 Intake.select :open @19:20:25.96 Intake @15.4psia, vacuum from 8.9 to 12.9psi @19:20:25.98 SS.reconfigure SSinhale.with maxSpeed: 35 @19:20:26.05 SS.seek 25ml @19:21:17.60 Stroke completed -- waiting for pressure to equalize ... @19:21:22.31 Intake.select :close @19:21:22.47 SSV.dial :exhaust @19:21:23.33 Exhaust.select :open @19:21:23.46 SS.reconfigure SSexhale @19:21:23.52 SS.seek 0ml @19:21:33.25 Exhaust.select :close @19:21:33.38 SSV.dial CBV @19:21:33.40
CBV.dial SSV @19:21:34.27 Allowing 130s for 25.0ml of remaining 325.0ml @19:21:34.28 Intake.select :open @19:21:36.47 Intake @15.5psia, vacuum from 9.0 to 13.0psi @19:21:36.49 SS.reconfigure SSinhale.with maxSpeed: 35 @19:21:36.56 SS.seek 25ml @19:22:26.96 Stroke completed -- waiting for pressure to equalize ... @19:22:31.27 Intake.select :close @19:22:31.42 SSV.dial :exhaust @19:22:32.30 Exhaust.select :open @19:22:32.43 SS.reconfigure SSexhale @19:22:32.49 SS.seek 0ml @19:22:42.10 Exhaust.select :close @19:22:42.23 SSV.dial CBV @19:22:42.26
CBV.dial SSV @19:22:43.14 Allowing 130s for 25.0ml of remaining 300.0ml @19:22:43.15 Intake.select :open @19:22:45.33 Intake @15.4psia, vacuum from 8.9 to 12.9psi @19:22:45.35 SS.reconfigure SSinhale.with maxSpeed: 35 @19:22:45.42 SS.seek 25ml @19:23:36.05 Stroke completed -- waiting for pressure to equalize ... @19:23:40.34 Intake.select :close @19:23:40.50 SSV.dial :exhaust @19:23:41.36 Exhaust.select :open @19:23:41.49 SS.reconfigure SSexhale @19:23:41.55 SS.seek 0ml @19:23:51.34 Exhaust.select :close @19:23:51.47 SSV.dial CBV @19:23:51.50
CBV.dial SSV @19:23:52.39 Allowing 130s for 25.0ml of remaining 275.0ml @19:23:52.40 Intake.select :open @19:23:54.58 Intake @15.3psia, vacuum from 8.8 to 12.8psi @19:23:54.60 SS.reconfigure SSinhale.with maxSpeed: 35 @19:23:54.67 SS.seek 25ml @19:24:45.32 Stroke completed -- waiting for pressure to equalize ... @19:24:49.62 Intake.select :close @19:24:49.78 SSV.dial :exhaust @19:24:50.65 Exhaust.select :open @19:24:50.78 SS.reconfigure SSexhale @19:24:50.84 SS.seek 0ml @19:25:00.43 Exhaust.select :close @19:25:00.56 SSV.dial CBV @19:25:00.59
CBV.dial SSV @19:25:01.45 Allowing 130s for 25.0ml of remaining 250.0ml @19:25:01.46 Intake.select :open @19:25:03.64 Intake @15.3psia, vacuum from 8.8 to 12.8psi @19:25:03.65 SS.reconfigure SSinhale.with maxSpeed: 35 @19:25:03.73 SS.seek 25ml @19:25:54.41 Stroke completed -- waiting for pressure to equalize ... @19:25:59.15 Intake.select :close @19:25:59.31 SSV.dial :exhaust @19:26:00.19 Exhaust.select :open @19:26:00.32 SS.reconfigure SSexhale @19:26:00.38 SS.seek 0ml @19:26:10.12 Exhaust.select :close @19:26:10.25 SSV.dial CBV @19:26:10.28
CBV.dial SSV @19:26:11.17 Allowing 130s for 25.0ml of remaining 225.0ml @19:26:11.18 Intake.select :open @19:26:13.36 Intake @15.4psia, vacuum from 8.9 to 12.9psi @19:26:13.38 SS.reconfigure SSinhale.with maxSpeed: 35 @19:26:13.46 SS.seek 25ml @19:26:56.87 Vacuum too high -- waiting up to 40s @19:26:59.02 Intake @15.0psia, vacuum from 8.5 to 12.5psi @19:26:59.04 SS.reconfigure SSinhale.with maxSpeed: 32 @19:26:59.11 SS.seek 25ml @19:27:06.96 Stroke completed -- waiting for pressure to equalize ... @19:27:10.03 Intake.select :close @19:27:10.18 SSV.dial :exhaust @19:27:11.05 Exhaust.select :open @19:27:11.18 SS.reconfigure SSexhale @19:27:11.24 SS.seek 0ml @19:27:20.83 Exhaust.select :close @19:27:20.96 SSV.dial CBV @19:27:20.99
CBV.dial SSV @19:27:21.88 Allowing 130s for 25.0ml of remaining 200.0ml @19:27:21.89 Intake.select :open @19:27:24.06 Intake @15.3psia, vacuum from 8.8 to 12.8psi @19:27:24.08 SS.reconfigure SSinhale.with maxSpeed: 32 @19:27:24.15 SS.seek 25ml @19:28:19.77 Stroke completed -- waiting for pressure to equalize ... @19:28:22.85 Intake.select :close @19:28:23.00 SSV.dial :exhaust @19:28:23.86 Exhaust.select :open @19:28:23.99 SS.reconfigure SSexhale @19:28:24.05 SS.seek 0ml @19:28:33.83 Exhaust.select :close @19:28:33.95 SSV.dial CBV @19:28:33.98
CBV.dial SSV @19:28:34.84 Allowing 130s for 25.0ml of remaining 175.0ml @19:28:34.85 Intake.select :open @19:28:37.03 Intake @15.4psia, vacuum from 8.9 to 12.9psi @19:28:37.05 SS.reconfigure SSinhale.with maxSpeed: 32 @19:28:37.12 SS.seek 25ml @19:29:32.18 Stroke completed -- waiting for pressure to equalize ... @19:29:35.68 Intake.select :close @19:29:35.82 SSV.dial :exhaust @19:29:36.70 Exhaust.select :open @19:29:36.83 SS.reconfigure SSexhale @19:29:36.89 SS.seek 0ml @19:29:46.51 Exhaust.select :close @19:29:46.64 SSV.dial CBV @19:29:46.67
CBV.dial SSV @19:29:47.56 Allowing 130s for 25.0ml of remaining 150.0ml @19:29:47.57 Intake.select :open @19:29:49.77 Intake @15.3psia, vacuum from 8.8 to 12.8psi @19:29:49.79 SS.reconfigure SSinhale.with maxSpeed: 32 @19:29:49.86 SS.seek 25ml @19:30:45.47 Stroke completed -- waiting for pressure to equalize ... @19:30:49.16 Intake.select :close @19:30:49.31 SSV.dial :exhaust @19:30:50.19 Exhaust.select :open @19:30:50.32 SS.reconfigure SSexhale @19:30:50.38 SS.seek 0ml @19:30:59.91 Exhaust.select :close @19:31:00.04 SSV.dial CBV @19:31:00.07
CBV.dial SSV @19:31:00.95 Allowing 130s for 25.0ml of remaining 125.0ml @19:31:00.96 Intake.select :open @19:31:03.14 Intake @15.3psia, vacuum from 8.8 to 12.8psi @19:31:03.16 SS.reconfigure SSinhale.with maxSpeed: 32 @19:31:03.24 SS.seek 25ml @19:31:58.52 Stroke completed -- waiting for pressure to equalize ... @19:32:02.19 Intake.select :close @19:32:02.35 SSV.dial :exhaust @19:32:03.22 Exhaust.select :open @19:32:03.35 SS.reconfigure SSexhale @19:32:03.41 SS.seek 0ml @19:32:12.99 Exhaust.select :close @19:32:13.11 SSV.dial CBV @19:32:13.14
CBV.dial SSV @19:32:14.02 Allowing 130s for 25.0ml of remaining 99.9ml @19:32:14.03 Intake.select :open @19:32:16.21 Intake @15.3psia, vacuum from 8.8 to 12.8psi @19:32:16.23 SS.reconfigure SSinhale.with maxSpeed: 32 @19:32:16.30 SS.seek 25ml @19:33:11.91 Stroke completed -- waiting for pressure to equalize ... @19:33:15.01 Intake.select :close @19:33:15.17 SSV.dial :exhaust @19:33:16.05 Exhaust.select :open @19:33:16.18 SS.reconfigure SSexhale @19:33:16.24 SS.seek 0ml @19:33:25.50 Exhaust.select :close @19:33:25.62 SSV.dial CBV @19:33:25.65
CBV.dial SSV @19:33:26.53 Allowing 130s for 25.0ml of remaining 74.9ml @19:33:26.54 Intake.select :open @19:33:28.72 Intake @15.3psia, vacuum from 8.8 to 12.8psi @19:33:28.74 SS.reconfigure SSinhale.with maxSpeed: 32 @19:33:28.82 SS.seek 25ml @19:34:24.69 Stroke completed -- waiting for pressure to equalize ... @19:34:28.76 Intake.select :close @19:34:28.92 SSV.dial :exhaust @19:34:29.81 Exhaust.select :open @19:34:29.94 SS.reconfigure SSexhale @19:34:30.00 SS.seek 0ml @19:34:39.21 Exhaust.select :close @19:34:39.35 SSV.dial CBV @19:34:39.37
CBV.dial SSV @19:34:40.26 Allowing 130s for 25.0ml of remaining 49.9ml @19:34:40.27 Intake.select :open @19:34:42.46 Intake @15.2psia, vacuum from 8.7 to 12.7psi @19:34:42.48 SS.reconfigure SSinhale.with maxSpeed: 32 @19:34:42.55 SS.seek 25ml @19:35:38.49 Stroke completed -- waiting for pressure to equalize ... @19:35:42.19 Intake.select :close @19:35:42.34 SSV.dial :exhaust @19:35:43.20 Exhaust.select :open @19:35:43.33 SS.reconfigure SSexhale @19:35:43.39 SS.seek 0ml @19:35:53.10 Exhaust.select :close @19:35:53.23 SSV.dial CBV @19:35:53.26
CBV.dial SSV @19:35:54.14 Allowing 130s for 24.9ml of remaining 24.9ml @19:35:54.15 Intake.select :open @19:35:56.33 Intake @15.3psia, vacuum from 8.8 to 12.8psi @19:35:56.35 SS.reconfigure SSinhale.with maxSpeed: 32 @19:35:56.43 SS.seek 24.95ml @19:36:51.41 Stroke completed -- waiting for pressure to equalize ... @19:36:55.09 Intake.select :close @19:36:55.24 SSV.dial :exhaust @19:36:56.12 Exhaust.select :open @19:36:56.25 SS.reconfigure SSexhale @19:36:56.31 SS.seek 0ml @19:37:06.21 Exhaust.select :close @19:37:06.34 Sampled 1500.0ml @19:37:06.35 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @19:37:06.37 Intake.select :close @19:37:06.46 CSV.dial :waste @19:37:06.54 CS.seek 0ml @19:37:06.63 SSV.dial CBV,avoiding: :exhaust @19:37:06.66
CBV.dial SSV @19:37:06.74 CSV.dial :air @19:37:07.59 CS.seek 10ml @19:37:08.83
CTV.dial :air @19:37:09.34 Gate.power :sampler,:OFF @19:37:09.72
delay 15 seconds @19:37:24.74 SSV.dial :spit,avoiding: :exhaust @19:37:26.23 CTV.dial CSV,avoiding: :intake @19:37:26.29
SS.seek 0ml @19:37:26.40 SSV.dial CBV,avoiding: :exhaust @19:37:34.72 CBV.dial between SSV and waste2 @19:37:35.15 CTV.dial CSV,avoiding: :air @19:37:35.18
CSV.dial CTV @19:37:35.70 CS.reconfigure CSpressurize @19:37:35.77 CS.seek 0ml @19:38:01.98 CSV.dial :air @19:38:02.52 CS.reconfigure CSfast @19:38:02.59 CS.seek 10ml @19:38:29.66 CSV.dial between air and CTV @19:38:30.04 CS.seek 2.762ml @19:38:49.61 CTV.dial CSV,avoiding: :air @19:38:49.64
CSV.dial CTV @19:38:50.00 CS.reconfigure CSpressurize @19:38:50.07 CS.seek 0ml @19:38:55.26 BubblePoint: Intake @69.1psia, Outlet @13.5psia @19:38:55.27 CBV.dial SSV @19:38:55.66 SS.reconfigure SSconfig @19:38:55.72 SS.seek 10ml @19:39:01.62 delay 15 seconds @19:39:16.74 Evacuated: Intake @39.9psia, Outlet @11.2psia @19:39:16.75 SSV.dial :spit,avoiding: :exhaust @19:39:18.26 SS.reconfigure SSexhale @19:39:18.33 SS.seek 0ml @19:39:23.73 SS.reconfigure SSfast @19:39:23.80 SS.seek 5ml @19:39:26.59 SSV.dial CBV,avoiding: :exhaust @19:39:26.62
CBV.dial SSV @19:39:28.12 CTV.dial :air,avoiding: :intake @19:39:29.67 CSV.dial CTV @19:39:29.71
SS.seek 10ml @19:39:30.70 CSV.dial :air @19:39:31.22 CS.reconfigure CSfast @19:39:31.28 CS.seek 0ml @19:39:32.47
SSV.dial :spit,avoiding: :exhaust @19:39:34.00 SS.reconfigure SSexhale @19:39:34.07 SS.seek 0ml @19:39:34.86 CS.coast @19:39:39.23
SS.reconfigure SSfast @19:39:39.30 SS.seek 5ml @19:39:42.14 SSV.dial CBV,avoiding: :exhaust @19:39:42.17
CBV.dial SSV @19:39:44.70 SSV.dial :spit,avoiding: :exhaust @19:39:46.22 SS.reconfigure SSconfig @19:39:46.29 SS.seek 0ml @19:39:49.58 SS.coast @19:39:49.64 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @19:39:49.65 Intake.select :close @19:39:49.76 CSV.dial :waste @19:39:49.88 SSV.dial CBV,avoiding: :exhaust @19:39:49.90
CBV.dial SSV @19:39:50.66 CS.seek 0ml @19:39:50.76 CSV.dial :air @19:39:51.43
CTV.dial :air @19:39:51.44 delay 15 seconds @19:39:51.63 CS.seek 10ml @19:40:06.47
SSV.dial :spit,avoiding: :exhaust @19:40:07.98 CTV.dial CSV,avoiding: :intake @19:40:08.02
SS.reconfigure SSexhale @19:40:08.13 SS.seek 0ml @19:40:08.53 SSV.dial CBV,avoiding: :exhaust @19:40:18.51 CBV.dial between SSV and waste2 @19:40:18.95 CSV.dial between air and CTV @19:40:19.32 CS.seek 8.295ml @19:40:24.67 CTV.dial CSV,avoiding: :air @19:40:24.70
CSV.dial CTV @19:40:25.06 CS.reconfigure CSpressurize @19:40:25.13 CS.seek 0ml @19:40:46.96 CSV.dial :air @19:40:47.91 CS.reconfigure CSfast @19:40:47.98 CS.seek 10ml @19:41:14.51 CSV.dial between air and CTV @19:41:14.90 CS.seek 3.047ml @19:41:33.52 CTV.dial CSV,avoiding: :air @19:41:33.55
CSV.dial CTV @19:41:33.91 CS.reconfigure CSpressurize @19:41:33.98 CS.seek 0ml @19:41:41.26 BubblePoint: Intake @68.8psia, Outlet @13.3psia @19:41:41.27 CBV.dial SSV @19:41:41.66 SS.reconfigure SSconfig @19:41:41.73 SS.seek 10ml @19:41:47.26 delay 15 seconds @19:42:02.38 Evacuated: Intake @41.9psia, Outlet @13.6psia @19:42:02.39 SSV.dial :spit,avoiding: :exhaust @19:42:03.92 SS.reconfigure SSexhale @19:42:03.99 SS.seek 0ml @19:42:09.24 SS.reconfigure SSfast @19:42:09.31 SS.seek 5ml @19:42:12.15 SSV.dial CBV,avoiding: :exhaust @19:42:12.18
CBV.dial SSV @19:42:13.70 CTV.dial :air,avoiding: :intake @19:42:15.27 CSV.dial CTV @19:42:15.32
SS.seek 10ml @19:42:16.30 CSV.dial :air @19:42:16.83 CS.reconfigure CSfast @19:42:16.89 CS.seek 0ml @19:42:17.81
SSV.dial :spit,avoiding: :exhaust @19:42:19.08 CS.coast @19:42:19.34
SS.reconfigure SSexhale @19:42:19.41 SS.seek 0ml @19:42:24.58 SS.reconfigure SSfast @19:42:24.65 SS.seek 5ml @19:42:27.48 SSV.dial CBV,avoiding: :exhaust @19:42:27.51
CBV.dial SSV @19:42:30.04 SSV.dial :spit,avoiding: :exhaust @19:42:31.57 SS.reconfigure SSconfig @19:42:31.64 SS.seek 0ml @19:42:35.16 SS.coast @19:42:35.22 Lysing: Adding 1.5ml lysis @19:42:35.23 CBV.dial between CSV and waste1,avoiding: :DBV @19:42:36.97 CTV.dial CSV @19:42:38.50 CSV.dial CSR @19:42:39.41 Pulling 1ml of CSR.lysis @19:42:39.42 CSR.select :lysis @19:42:39.57 CS.reconfigure CSconfig @19:42:39.63 CS.seek 1ml @19:42:43.14 delay 5 seconds @19:42:48.16 CSR.select :air @19:42:48.34 CS.seek 6ml @19:43:02.05 delay 2 seconds @19:43:04.07 CSV.dial :waste1 @19:43:05.58 CS.reconfigure CSfast @19:43:05.64 CS.seek :empty @19:43:21.85 CSV.dial CSR @19:43:23.43 Pulling 1.5ml of CSR.lysis @19:43:23.44 CSR.select :lysis @19:43:23.59 CS.reconfigure CSconfig @19:43:23.65 CS.seek 1.5ml @19:43:28.58 delay 5 seconds @19:43:33.60 CSR.select :air @19:43:33.78 CS.seek 4.5ml @19:43:42.64 delay 2 seconds @19:43:44.66 CSV.dial CTV @19:43:45.19 CS.seek 1.275ml @19:43:55.21 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @19:43:55.24 CH.seek 85C,8:00 @19:48:57.02 CH.hold 5:00,85C @19:53:57.18 Collection Puck Heater at 83.9C after lysing @19:53:57.20 CS.seek 0ml @19:54:01.46 CTV.dial between spare and air,avoiding: :intake @19:54:02.68 CSV.dial :air @19:54:03.20 CS.seek 5ml @19:54:16.98 CSV.dial CTV @19:54:17.53 delay 2 seconds @19:54:19.55 CSV.dial :air @19:54:20.07 delay 2 seconds @19:54:22.09 CSV.dial CTV @19:54:22.62 delay 2 seconds @19:54:24.64 CSV.dial :air @19:54:25.17 delay 2 seconds @19:54:27.20 CS.seek 0ml @19:54:41.09 Recovering Lysate into Collection syringe @19:54:41.10 CSV.dial CBV @19:54:42.31 CBV.dial CSV @19:54:42.73 CS.seek 5ml @19:54:56.49 delay 5 seconds @19:55:01.51 CTV.dial :air @19:55:02.09 CS.seek 6.999ml @19:55:08.18 delay 5 seconds @19:55:13.20 CSV.dial :air2 @19:55:13.71 CS.seek 10ml @19:55:22.49 CSV.dial between air and CTV,avoiding: CBV @19:55:23.84 Lysing Complete @19:55:23.92 Storing sh1 puck in Collection Clamp to tube 1 @19:55:24.10 SC.reconfigure SCtight @19:55:24.16 SC.seek 1 @19:55:26.30 SC.reconfigure SCloose @19:55:26.95 Elbow.reconfigure TightElbow @19:55:27.01 Elbow.seek 1 @19:55:28.92 Forearm.reconfigure TightForearm @19:55:28.99 Forearm.seek 1 + 700 counts @19:55:30.08 SE.reconfigure SEpick @19:55:30.17 SE.seek beamBreak @19:55:33.15 SE.configure SEconfig @19:55:33.21 SE.seek :place @19:55:33.80 Forearm.reconfigure LooseForearm @19:55:33.88 Forearm.seek :retract @19:55:34.83 Elbow.seek CC @19:55:36.45 Elbow.reconfigure LooseElbow @19:55:36.55 Forearm.seek CC @19:55:37.63 Hand.close @19:55:38.35 CC.seek :open @19:55:42.81 Forearm.seek :retract @19:55:43.88 Elbow.reconfigure TightElbow @19:55:43.94 Elbow.seek 1 @19:55:45.88 Elbow.reconfigure LimpElbow @19:55:45.95 Forearm.seek 1 @19:55:46.98 Elbow.hold @19:55:47.06 Elbow.reconfigure TightElbow @19:55:47.12 Hand.open @19:55:47.97 Forearm.force -20 @19:55:48.02 Forearm.seek 1 + 348 counts @19:55:48.35 Forearm.reconfigure TightForearm @19:55:48.42 Forearm.seek 1 + 700 counts @19:55:48.77 SE.seek :down @19:55:49.90
Forearm.reconfigure LooseForearm @19:55:49.96 Forearm.seek :retract @19:55:51.62 SE.coast @19:55:51.67
SC.coast @19:55:51.72 Tranferring lysate to PRV @19:55:51.73 CSV.dial :air @19:55:52.09 CS.seek 10ml @19:55:52.18 CSV.dial between air and CTV @19:55:52.56 AIV.dial :bypass @19:55:52.65 Beginning samplePassC @19:55:52.66 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @19:55:52.75 Hand.open @19:55:53.10 Elbow.seek FlushPuck::Garage @19:55:55.26 Elbow.reconfigure LooseElbow @19:55:55.37 Forearm.seek :garage @19:55:56.42 Hand.close @19:55:57.33 Forearm.seek :retract @19:55:58.82 Elbow.reconfigure TightElbow @19:55:58.88 Elbow.seek CC @19:56:01.82 Elbow.reconfigure LooseElbow @19:56:01.94 Forearm.reconfigure TightForearm @19:56:02.00 Forearm.seek CC @19:56:03.34 CC.seek :closed @19:56:07.70 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.40 @19:56:07.79 Hand.open @19:56:08.66 Forearm.reconfigure LooseForearm @19:56:08.72 Forearm.seek :retract @19:56:09.76 AWV.select :air @19:56:09.78 AIV.dial :bypass @19:56:09.81 PSV.dial PSR @19:56:11.35 Pulling 1.5ml of PSR.flush @19:56:11.36 PSR.select :flush @19:56:11.51 PS.seek 1.5ml @19:56:27.71 delay 5 seconds @19:56:32.73 PSV.dial :waste1 @19:56:33.24 PS.seek 0ml @19:56:49.48 PSV.dial PSR @19:56:50.01 PSR.select :air @19:56:50.15 PS.seek 2.5ml @19:57:16.77 PRV.dial CBV @19:57:16.80
CBV.dial PRV @19:57:17.73 PSV.dial :mixloop @19:57:18.88 CTV.dial :air,avoiding: :intake @19:57:18.89 PTV.dial :PRVmixing @19:57:19.01 PS.seek 0ml @19:57:45.64 PSV.dial :air @19:57:46.16 PS.seek 2.5ml @19:58:12.83 PSV.dial :mixloop @19:58:13.35 PS.seek 0ml @19:58:39.86 PSV.dial :air @19:58:40.38 PS.seek 2.5ml @19:59:07.01 PSV.dial :mixloop @19:59:07.54 PS.seek 0ml @19:59:33.77 Garaging FlushPuck in Collection Clamp @19:59:33.86 Elbow.reconfigure TightElbow @19:59:33.92 Elbow.seek CC @19:59:34.15 Elbow.reconfigure LooseElbow @19:59:34.26 Forearm.seek CC @19:59:35.33 Hand.close @19:59:36.10 CC.seek :open @19:59:40.48 Forearm.seek :retract @19:59:41.52 Elbow.reconfigure TightElbow @19:59:41.58 Elbow.seek :garage @19:59:44.67 Elbow.reconfigure LooseElbow @19:59:44.78 Forearm.reconfigure TightForearm @19:59:44.83 Forearm.seek :garage @19:59:45.99 Hand.open @19:59:46.79 Forearm.reconfigure LooseForearm @19:59:46.85 Forearm.seek :retract @19:59:47.90 Connecting PS to CS to transfer sample @19:59:48.33 CBV.dial AIV @19:59:48.38 AIV.dial :bypass @19:59:48.40 PSV.dial :mixloop @19:59:48.43 PTV.dial :PRVmixing @19:59:48.46 PRV.dial AIV @19:59:48.48 CTV.dial :air,avoiding: :intake @19:59:48.51 Loading lyfil puck from tube 2 into Collection Clamp @19:59:48.76 SC.reconfigure SCtight @19:59:48.82 SC.seek 2 @19:59:51.03 SC.reconfigure SCloose @19:59:51.25 Elbow.reconfigure TightElbow @19:59:51.32 Elbow.seek 2 @19:59:53.36 Forearm.reconfigure TightForearm @19:59:53.42 Forearm.seek 2 + 700 counts @19:59:54.49 SE.reconfigure SEpick @19:59:54.58 SE.seek beamBreak @19:59:57.72 SE.configure SEconfig @19:59:57.88 Elbow.seek 2 @19:59:58.29 Forearm.seek 2 + 300 counts @19:59:58.51 Forearm.reconfigure LooseForearm @19:59:58.57 Hand.close @19:59:59.36 SE.seek :down @19:59:59.56
Forearm.seek :retract @20:00:00.55 Elbow.seek CC @20:00:02.12 SE.coast @20:00:02.25
Elbow.reconfigure LooseElbow @20:00:02.36 Forearm.reconfigure TightForearm @20:00:02.42 Forearm.seek CC @20:00:03.75 CC.seek :closed @20:00:08.01 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.48 @20:00:08.03 Hand.open @20:00:08.92 Forearm.reconfigure LooseForearm @20:00:08.98 Forearm.seek :retract @20:00:10.02 SC.coast @20:00:10.06 CTV.dial CSV,avoiding: :intake @20:00:11.59 CSV.dial CTV @20:00:11.92 CS.seek 5ml @20:00:25.81 delay 1:20 @20:01:45.84 CS.seek 2.5ml @20:01:53.25 delay 40 seconds @20:02:33.28 CS.seek 0ml @20:02:41.00 delay 1:00 @20:03:41.02 PSV.dial :air @20:03:41.54 PS.seek 2.5ml @20:04:07.83 PSV.dial :mixloop @20:04:08.35 delay 3 seconds @20:04:11.37 PSV.dial :air @20:04:11.88 delay 2 seconds @20:04:13.90 PSV.dial :mixloop @20:04:14.41 delay 3 seconds @20:04:17.43 PSV.dial :waste1 @20:04:18.94 PS.seek :empty @20:04:45.12 CBV.dial :waste1 @20:04:45.67 delay 5 seconds @20:04:50.69 CBV.dial AIV @20:04:51.23 delay 10 seconds @20:05:01.26 CBV.dial :waste1 @20:05:01.81 delay 10 seconds @20:05:11.84 CBV.dial AIV @20:05:12.84 delay 10 seconds @20:05:22.87 CTV.dial CSV,avoiding: :intake @20:05:22.88 CBV.dial between AIV and waste1 @20:05:23.30 CSV.dial :air @20:05:23.83 CS.seek 10ml @20:05:50.33 CSV.dial CTV @20:05:50.88 CS.seek 0ml @20:06:17.75 CBV.dial :waste1 @20:06:18.10 delay 10 seconds @20:06:28.13 CTV.dial :air,avoiding: :intake @20:06:29.73 Storing lyfil puck in Collection Clamp to tube 1 @20:06:29.90 SC.reconfigure SCtight @20:06:29.96 SC.seek 1 @20:06:32.12 SC.reconfigure SCloose @20:06:32.33 Elbow.reconfigure TightElbow @20:06:32.39 Elbow.seek 1 @20:06:34.31 Forearm.reconfigure TightForearm @20:06:34.37 Forearm.seek 1 + 700 counts @20:06:35.45 SE.reconfigure SEpick @20:06:35.54 SE.seek beamBreak @20:06:38.69 SE.configure SEconfig @20:06:38.85 Elbow.reconfigure LimpElbow @20:06:38.91 Forearm.reconfigure LooseForearm @20:06:38.97 Forearm.seek 1 @20:06:39.35 Elbow.hold @20:06:39.43 Elbow.reconfigure TightElbow @20:06:39.53 SE.seek 0.07 Pucks below Top Plate @20:06:40.00 Forearm.force -20 @20:06:40.06 Forearm.seek 1 + 316 counts @20:06:40.39 Forearm.reconfigure TightForearm @20:06:40.46 Forearm.seek 1 + 700 counts @20:06:40.77 SE.seek 1.07 Pucks below Top Plate @20:06:41.36 Forearm.reconfigure LooseForearm @20:06:41.42 Forearm.seek :retract @20:06:42.34 Elbow.seek CC @20:06:43.95 Elbow.reconfigure LooseElbow @20:06:44.05 Forearm.seek CC @20:06:45.13 Hand.close @20:06:45.86 CC.seek :open @20:06:50.31 Forearm.seek :retract @20:06:51.37 Elbow.reconfigure TightElbow @20:06:51.43 Elbow.seek 1 @20:06:53.33 Elbow.reconfigure LimpElbow @20:06:53.40 Forearm.seek 1 @20:06:54.44 Elbow.hold @20:06:54.52 Elbow.reconfigure TightElbow @20:06:54.57 Hand.open @20:06:55.47 Forearm.force -20 @20:06:55.53 Forearm.seek 1 + 349 counts @20:06:55.84 Forearm.reconfigure TightForearm @20:06:55.90 Forearm.seek 1 + 700 counts @20:06:56.71 SE.seek :down @20:06:57.84
Forearm.reconfigure LooseForearm @20:06:57.90 Forearm.seek :retract @20:06:59.47 SE.coast @20:06:59.52
SC.coast @20:06:59.57 PSV.dial :air @20:07:00.80 PS.seek 0.499ml @20:07:06.71 delay 3 seconds @20:07:09.73 PSV.dial :mixloop @20:07:10.23 PRV.dial :air @20:07:10.74 delay 3 seconds @20:07:13.76 PRV.dial CBV @20:07:14.29 CBV.dial AIV @20:07:14.81 PRV.dial between CBV and air @20:07:15.18 PRV.dial :air @20:07:15.59 PS.reconfigure PSslow @20:07:15.65 PS.seek 0.599ml @20:07:18.21 PRV.dial :waste2 @20:07:19.05 PS.reconfigure PSconfig @20:07:19.11 PS.seek :empty @20:07:26.15 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:07:26.23 Hand.open @20:07:26.59 Elbow.seek FlushPuck::Garage @20:07:28.68 Elbow.reconfigure LooseElbow @20:07:28.79 Forearm.seek :garage @20:07:29.84 Hand.close @20:07:30.72 Forearm.seek :retract @20:07:31.84 Elbow.reconfigure TightElbow @20:07:31.90 Elbow.seek CC @20:07:34.83 Elbow.reconfigure LooseElbow @20:07:34.94 Forearm.reconfigure TightForearm @20:07:35.00 Forearm.seek CC @20:07:36.37 CC.seek :closed @20:07:40.71 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.50 @20:07:40.79 Hand.open @20:07:41.55 Forearm.reconfigure LooseForearm @20:07:41.61 Forearm.seek :retract @20:07:42.73 Clearing PML for modified lysate @20:07:42.74 CTV.dial :air,avoiding: :intake @20:07:42.76 PRV.dial :waste2 @20:07:42.79 PTV.dial :PRV,avoiding: :puck @20:07:43.63 delay 2 seconds @20:07:45.65 PTV.dial :PRVmixing,avoiding: :puck @20:07:46.49 delay 2 seconds @20:07:48.51 PSV.dial :PML @20:07:48.52 delay 2 seconds @20:07:50.55 PRV.dial :air @20:07:51.38 delay 2 seconds @20:07:53.45 PS.seek 1.499ml @20:08:09.59 delay 5 seconds @20:08:14.62 PS.seek 2.5ml @20:08:25.67 PSV.dial :air @20:08:26.20 PS.seek 0ml @20:08:52.42 mixing lysate with speDil @20:08:52.43 PSV.dial :PML @20:08:52.95 CBV.dial :PRV @20:08:52.96 PRV.dial :air @20:08:52.99 delay 2 seconds @20:08:55.02 PRV.dial :speDil @20:08:55.54 delay 2 seconds @20:08:57.62 PS.reconfigure PSslow1 @20:08:57.69 PS.seek 0.225ml @20:09:21.71 delay 5 seconds @20:09:26.73 PRV.dial CBV @20:09:27.60 delay 2 seconds @20:09:29.67 PS.seek 0.475ml @20:09:55.46 delay 5 seconds @20:10:00.48 PRV.dial :air @20:10:01.01 delay 2 seconds @20:10:03.04 PS.seek 0.965ml @20:10:53.12 delay 10 seconds @20:11:03.15 PRV.dial CBV @20:11:03.67 CTV.dial :air,avoiding: :intake @20:11:03.68 PSV.dial :air @20:11:04.20 PS.reconfigure PSconfig @20:11:04.27 PS.seek 2.5ml @20:11:20.59 delay 3 seconds @20:11:23.61 PSV.dial PTV @20:11:24.45 PTV.dial :PRV,avoiding: :puck @20:11:25.31 PS.seek 0ml @20:11:51.50 PSV.dial :air @20:11:52.34 PS.seek 2.5ml @20:12:18.71 delay 3 seconds @20:12:21.73 PSV.dial PTV @20:12:22.57 PTV.dial :PRV,avoiding: :puck @20:12:22.59 PS.seek 0ml @20:12:48.93 Pushing modified lysate into AIV Injection Loop @20:12:48.94 CBV.dial AIV @20:12:48.97 AIV.dial :bypass @20:12:48.99 PRV.dial CBV @20:12:49.01 PSV.dial :air @20:12:49.87 PS.seek 2.5ml @20:13:16.34 delay 3 seconds @20:13:19.36 PSV.dial :PML @20:13:19.87 PTV.dial :PRVmixing,avoiding: :puck @20:13:20.77 PS.reconfigure PSslow1 @20:13:20.83 PS.seek 1.74ml @20:14:38.22 delay 5 seconds @20:14:43.24 AIV.dial :sample @20:14:43.88 AWV.select :waste @20:14:44.05 PS.reconfigure PSslow2 @20:14:44.11 PS.seek 1.22ml @20:16:31.62 delay 15 seconds @20:16:46.64 AIV.dial :bypass @20:16:47.27 PS.reconfigure PSconfig @20:16:47.33 PS.seek 0ml @20:17:00.61 PSV.dial :air @20:17:01.14 PS.seek 2.5ml @20:17:27.49 PSV.dial :PML @20:17:28.01 PS.seek 0ml @20:17:54.24 AIV.dial :coilToMFB @20:17:54.26 AWV.select :air @20:17:54.44 Extracting Nucleic Acid from AIV @20:17:54.47 SPE.hold 2:46:40,55C @20:17:54.51
Beginning samplePassD @20:17:54.56 ASV.dial :SPEdelivery @20:17:54.69
AWV.select :air @20:17:54.70 AIV.dial :bypass @20:17:54.73 PSV.dial PSR @20:17:55.05 delay 20 seconds @20:17:56.02
Pulling 1.5ml of PSR.killMFB @20:17:56.03 PSR.select :killMFB @20:17:56.18 PS.seek 1.5ml @20:18:13.12 delay 5 seconds @20:18:15.07 primeLysateINT @20:18:15.08 AWV.select :air @20:18:15.10 AIV.dial :coilToMFB @20:18:15.13 ATV.dial AIV @20:18:17.11 AS.reconfigure ASslow1 @20:18:17.17 AS.seek 0.299ml @20:18:18.14
PSV.dial :waste1 @20:18:18.67 PS.seek 0ml @20:18:36.00 PSV.dial PSR @20:18:36.54 PSR.select :air @20:18:36.67 PS.seek 2.5ml @20:19:04.37 PRV.dial CBV @20:19:04.40
CBV.dial PRV @20:19:04.42 PSV.dial :mixloop @20:19:05.65 CTV.dial :air,avoiding: :intake @20:19:05.66 PTV.dial :PRVmixing @20:19:05.70 PS.seek 0ml @20:19:17.86 delay 10 seconds @20:19:27.89 ATV.dial :air2 @20:19:28.81 AS.seek 0.329ml @20:19:33.56
PSV.dial PSR @20:19:34.83 Pulling 1.5ml of PSR.flush @20:19:34.84 PSR.select :flush @20:19:34.99 PS.seek 1.5ml @20:19:35.13 ATV.dial :waste2 @20:19:35.75 AS.reconfigure ASnorm @20:19:35.81 AS.seek :empty @20:19:52.00
delay 5 seconds @20:19:52.84 Lysate primed! @20:19:52.85 AFV.select :holdingCoil @20:19:52.86 ATV.dial :air2 @20:19:53.56 AS.reconfigure ASslow1 @20:19:53.62 AS.seek 0.069ml @20:19:57.02
PSV.dial :waste1 @20:19:57.56 PS.seek 0ml @20:20:08.03 delay 3 seconds @20:20:11.05 ATV.dial :AIV @20:20:11.97 AS.seek 0.469ml @20:20:14.50
PSV.dial PSR @20:20:15.04 PSR.select :air @20:20:15.17 PS.seek 2.5ml @20:20:42.84 PRV.dial CBV @20:20:42.87
CBV.dial PRV @20:20:42.89 PSV.dial :mixloop @20:20:44.11 CTV.dial :air,avoiding: :intake @20:20:44.12 PTV.dial :PRVmixing @20:20:44.16 PS.seek 0ml @20:21:11.69 PSV.dial PSR @20:21:12.94 Pulling 1.5ml of PSR.flush @20:21:12.95 PSR.select :flush @20:21:13.10 PS.seek 1.5ml @20:21:29.69 delay 5 seconds @20:21:32.70 delay 5 seconds @20:21:34.71
PSV.dial :waste1 @20:21:35.48 PS.seek 0ml @20:21:37.76 Solid Phase Extraction Heater at 49.8C @20:21:37.77 ATV.dial ASV @20:21:38.22 ASV.dial :SPEdelivery @20:21:38.31 AS.seek 0.041ml @20:21:52.12
PSV.dial PSR @20:21:52.65 PSR.select :air @20:21:52.78 PS.seek 2.5ml @20:22:19.90 PRV.dial CBV @20:22:19.92
CBV.dial PRV @20:22:19.95 PSV.dial :mixloop @20:22:21.16 CTV.dial :air,avoiding: :intake @20:22:21.17 PTV.dial :PRVmixing @20:22:21.21 PS.seek 0ml @20:22:48.40 PSV.dial PSR @20:22:49.64 Pulling 1.5ml of PSR.flush @20:22:49.65 PSR.select :flush @20:22:49.80 PS.seek 1.5ml @20:23:04.64 delay 10 seconds @20:23:06.34
delay 5 seconds @20:23:11.36 PSV.dial :waste1 @20:23:11.87 PS.seek 0ml @20:23:14.66 rinseLysateLineINT @20:23:14.67 AFV.select :carrierSolution @20:23:14.79 delay 2 seconds @20:23:16.81 AS.reconfigure ASconfig @20:23:16.88 AS.seek 1ml @20:23:28.46
PSV.dial PSR @20:23:29.00 PSR.select :air @20:23:29.13 PS.seek 2.5ml @20:23:55.78 PRV.dial CBV @20:23:55.80
CBV.dial PRV @20:23:56.26 PSV.dial :mixloop @20:23:57.48 CTV.dial :air,avoiding: :intake @20:23:57.49 PTV.dial :PRVmixing @20:23:57.53 PS.reconfigure PSslow @20:23:57.60 PS.seek 1.2ml @20:24:12.28 delay 1 second @20:24:13.30 AFV.select :holdingCoil @20:24:13.40 delay 1 second @20:24:14.42 ATV.dial AIV @20:24:15.00 AIV.dial :coilToMFB @20:24:15.01 AWV.select :waste @20:24:15.15 AS.reconfigure ASslow0 @20:24:15.20 AS.seek 0ml @20:24:24.44
PSV.dial :waste1 @20:24:25.95 PS.reconfigure PSconfig @20:24:26.02 PS.seek 0ml @20:24:39.16 PSV.dial :air @20:24:40.33 PS.seek 2.5ml @20:25:06.91 PSV.dial :mixloop @20:25:07.41 PS.reconfigure PSslow @20:25:07.48 PS.seek 0ml @20:25:56.11 ATV.dial :air2 @20:25:56.81 AS.reconfigure ASslow1 @20:25:56.87 AS.seek 0.05ml @20:25:58.50
PSV.dial :air @20:25:59.01 PS.reconfigure PSconfig @20:25:59.07 PS.seek 2.5ml @20:26:07.19 ATV.dial :AIV @20:26:08.05 AS.seek 0.01ml @20:26:16.31 ATV.dial :waste @20:26:18.03 AS.seek :empty @20:26:20.50 ethanol wash @20:26:20.51 ATV.dial :air2 @20:26:21.83 AS.reconfigure ASslow2 @20:26:21.89 AS.seek 0.009ml @20:26:25.65
PSV.dial :mixloop @20:26:26.16 PS.reconfigure PSslow @20:26:26.22 PS.seek 0ml @20:26:32.22 delay 3 seconds @20:26:35.24 Pulling 0.4ml of Ethanol Wash @20:26:35.25 ATV.dial :ethanol @20:26:36.11 AS.reconfigure ASslow1 @20:26:36.17 AS.seek 0.409ml @20:27:17.28
Cleaning Collection Syringe @20:27:17.29 CSV.dial CSR @20:27:17.88 Pulling 4ml of CSR.flush @20:27:17.89 CSR.select :flush @20:27:18.04 CS.seek 4ml @20:27:29.39 delay 5 seconds @20:27:34.41 CSR.select :air @20:27:34.55 CS.seek 10ml @20:27:50.95 CSV.dial CTV @20:27:50.97
CTV.dial CSV @20:27:52.58 CBV.dial :waste1 @20:27:53.14 CS.seek 0ml @20:27:56.88 delay 3 seconds @20:27:59.90 ATV.dial :air1 @20:28:00.33 delay 3 seconds @20:28:03.40 AS.seek 0.434ml @20:28:08.72 delay 3 seconds @20:28:11.75 ATV.dial :ethanol @20:28:12.37 AS.seek 0.419ml @20:28:15.63 delay 3 seconds @20:28:18.65 ATV.dial ASV @20:28:19.67
CSV.dial CSR @20:28:20.03 AS.seek 0.092ml @20:28:20.25
Pulling 4ml of CSR.flush @20:28:20.26 CSR.select :flush @20:28:20.41 CS.seek 4ml @20:28:31.61 delay 5 seconds @20:28:36.63 CSR.select :air @20:28:36.77 CS.seek 10ml @20:28:53.11 CSV.dial CTV @20:28:53.13
CTV.dial CSV @20:28:53.69 CBV.dial :waste1 @20:28:53.71 CS.seek 0ml @20:29:20.28 Garaging FlushPuck in Collection Clamp @20:29:20.83 Elbow.reconfigure TightElbow @20:29:20.89 Elbow.seek CC @20:29:21.12 Elbow.reconfigure LooseElbow @20:29:21.23 Forearm.seek CC @20:29:22.32 Hand.close @20:29:23.13 CC.seek :open @20:29:26.06 delay 3 seconds @20:29:27.53
Forearm.seek :retract @20:29:28.60 Elbow.reconfigure TightElbow @20:29:28.66 Elbow.seek :garage @20:29:29.08 ATV.dial :waste2 @20:29:29.86 AS.reconfigure ASconfig @20:29:29.92 AS.seek :empty @20:29:31.79
Elbow.reconfigure LooseElbow @20:29:31.91 Forearm.reconfigure TightForearm @20:29:31.97 Forearm.seek :garage @20:29:33.12 Hand.open @20:29:33.95 Forearm.reconfigure LooseForearm @20:29:34.01 Forearm.seek :retract @20:29:35.09 Whole Cell Archive sampling 1500ml @20:29:35.10 Loading wcr puck from tube 2 into Collection Clamp @20:29:35.36 SC.reconfigure SCtight @20:29:35.42 SC.seek 2 @20:29:35.58 ATV.dial :air2 @20:29:36.02 AS.reconfigure ASslow1 @20:29:36.08 AS.seek 1ml @20:29:37.59 SC.reconfigure SCloose @20:29:37.82 Elbow.reconfigure TightElbow @20:29:37.88 Elbow.seek 2 @20:29:39.95 Forearm.reconfigure TightForearm @20:29:40.02 Forearm.seek 2 + 700 counts @20:29:41.02 SE.reconfigure SEpick @20:29:41.11 SE.seek beamBreak @20:29:44.25 SE.configure SEconfig @20:29:44.41 Elbow.seek 2 @20:29:44.60 Forearm.seek 2 + 300 counts @20:29:44.89 Forearm.reconfigure LooseForearm @20:29:44.95 Hand.close @20:29:45.78 SE.seek :down @20:29:45.97 Forearm.seek :retract @20:29:46.99 Elbow.seek CC @20:29:48.68 Elbow.reconfigure LooseElbow @20:29:48.80 SE.coast @20:29:48.83 Forearm.reconfigure TightForearm @20:29:48.92 Forearm.seek CC @20:29:50.26 CC.seek :closed @20:29:54.54 Collection Clamp onSpring/offSpring for 5/30 samples, on/off torque 2.39 @20:29:54.55 Hand.open @20:29:55.45 Forearm.reconfigure LooseForearm @20:29:55.52 Forearm.seek :retract @20:29:56.59 SC.coast @20:29:56.64 collectSample 1500ml w/bubble point of 55psi @20:29:56.66 Priming 7ml @20:29:56.68 Gate.power :sampler,:ON @20:29:56.70 Intake.select :close @20:29:56.73 Exhaust.select :close @20:29:56.76 SS.hold @20:29:56.98 Calibrating pressure sensors @20:29:56.99 CTV.dial :air @20:29:58.55 SSV.dial :spit,avoiding: :exhaust @20:29:58.58 SS.reconfigure SSfast @20:29:58.65 SS.seek 5ml @20:30:01.65 SSV.dial CBV @20:30:01.68 CBV.dial SSV @20:30:03.30 delay 3.8 seconds @20:30:07.21 SSV.dial :spit @20:30:08.73 SS.seek 0ml @20:30:11.40 SSV.dial CBV @20:30:12.92 CTV.dial :intake @20:30:13.81 Waiting for external sampler to finish priming... @20:30:13.82 delayUntil "20:34:56.70" @20:32:57.69 delay 5 seconds @20:33:02.71 ATV.dial ASV @20:33:03.74 ASV.dial :SPEdelivery @20:33:03.76 AS.seek 0.02ml @20:34:56.72 SSV.dial CBV @20:34:56.75 CBV.dial SSV @20:34:56.78 Allowing 40s for 7.0ml of remaining 7.0ml @20:34:56.80 Intake.select :open @20:34:58.98 Intake @15.5psia, vacuum from 9.0 to 13.0psi @20:34:58.99 SS.reconfigure SSinhale.with maxSpeed: 150 @20:34:59.07 SS.seek 7ml @20:35:05.13 Stroke completed -- waiting for pressure to equalize ... @20:35:21.08 Intake.select :close @20:35:21.23 SSV.dial :exhaust @20:35:22.61 Exhaust.select :open @20:35:22.74 SS.reconfigure SSexhale @20:35:22.80 SS.seek 0ml @20:35:26.77 Exhaust.select :close @20:35:26.91 Sampling 1500.0ml @20:35:26.94 Intake.select :close @20:35:26.96 Exhaust.select :close @20:35:26.99 SS.reconfigure SSconfig @20:35:27.06 SS.hold @20:35:27.27 CTV.dial :intake @20:35:27.29 SSV.dial CBV @20:35:27.32 CBV.dial SSV @20:35:28.22 Allowing 130s for 25.0ml of remaining 1500.0ml @20:35:28.24 Intake.select :open @20:35:30.43 Intake @15.7psia, vacuum from 9.2 to 13.2psi @20:35:30.44 SS.reconfigure SSinhale.with maxSpeed: 150 @20:35:30.52 SS.seek 25ml @20:35:35.63 Vacuum too high -- waiting up to 40s @20:35:45.15 Intake @15.5psia, vacuum from 9.0 to 13.0psi @20:35:45.17 SS.reconfigure SSinhale.with maxSpeed: 135 @20:35:45.24 SS.seek 25ml @20:35:48.73 Vacuum too high -- waiting up to 40s @20:35:59.43 SS.reconfigure SSinhale.with maxSpeed: 122 @20:35:59.50 SS.seek 25ml @20:36:03.38 Vacuum too high -- waiting up to 40s @20:36:13.87 SS.reconfigure SSinhale.with maxSpeed: 110 @20:36:13.94 SS.seek 25ml @20:36:18.29 Vacuum too high -- waiting up to 40s @20:36:21.14 delay 5 seconds @20:36:26.20 ATV.dial :air2 @20:36:27.30 AS.seek 0.52ml @20:36:28.20 Intake @15.5psia, vacuum from 9.0 to 13.0psi @20:36:28.22 SS.reconfigure SSinhale.with maxSpeed: 99 @20:36:28.29 SS.seek 25ml @20:36:31.70 Stroke completed -- waiting for pressure to equalize ... @20:36:40.92 Intake.select :close @20:36:41.09 SSV.dial :exhaust @20:36:41.96 Exhaust.select :open @20:36:42.10 SS.reconfigure SSexhale @20:36:42.17 SS.seek 0ml @20:36:51.37 Exhaust.select :close @20:36:51.48 SSV.dial CBV @20:36:51.51 CBV.dial SSV @20:36:52.42 Allowing 130s for 25.0ml of remaining 1475.0ml @20:36:52.44 Intake.select :open @20:36:54.63 Intake @15.6psia, vacuum from 9.1 to 13.1psi @20:36:54.64 SS.reconfigure SSinhale.with maxSpeed: 99 @20:36:54.72 SS.seek 25ml @20:36:59.26 Vacuum too high -- waiting up to 40s @20:37:05.36 Intake @15.4psia, vacuum from 8.9 to 12.9psi @20:37:05.39 SS.reconfigure SSinhale.with maxSpeed: 89 @20:37:05.46 SS.seek 25ml @20:37:09.01 Vacuum too high -- waiting up to 40s @20:37:15.32 SS.reconfigure SSinhale.with maxSpeed: 80 @20:37:15.38 SS.seek 25ml @20:37:20.05 Vacuum too high -- waiting up to 40s @20:37:26.84 Intake @15.3psia, vacuum from 8.8 to 12.8psi @20:37:26.86 SS.reconfigure SSinhale.with maxSpeed: 72 @20:37:26.93 SS.seek 25ml @20:37:32.79 Vacuum too high -- waiting up to 40s @20:37:40.70 Intake @15.4psia, vacuum from 8.9 to 12.9psi @20:37:40.72 SS.reconfigure SSinhale.with maxSpeed: 65 @20:37:40.80 SS.seek 25ml @20:37:47.07 Vacuum too high -- waiting up to 40s @20:37:54.33 Intake @15.3psia, vacuum from 8.8 to 12.8psi @20:37:54.35 SS.reconfigure SSinhale.with maxSpeed: 59 @20:37:54.42 SS.seek 25ml @20:37:56.53 Stroke completed -- waiting for pressure to equalize ... @20:38:01.49 Intake.select :close @20:38:01.65 SSV.dial :exhaust @20:38:02.54 Exhaust.select :open @20:38:02.67 SS.reconfigure SSexhale @20:38:02.73 SS.seek 0ml @20:38:08.16 delay 5 seconds @20:38:12.14 Exhaust.select :close @20:38:12.27 SSV.dial CBV @20:38:12.30 CBV.dial SSV @20:38:13.18 Allowing 130s for 25.0ml of remaining 1450.0ml @20:38:13.20 Intake.select :open @20:38:13.27 ATV.dial ASV @20:38:14.30 ASV.dial :wasteR @20:38:14.69 AS.seek 0.3ml @20:38:15.39 Intake @15.5psia, vacuum from 9.0 to 13.0psi @20:38:15.40 SS.reconfigure SSinhale.with maxSpeed: 59 @20:38:15.48 SS.seek 25ml @20:38:21.94 Vacuum too high -- waiting up to 40s @20:38:26.65 Intake @15.3psia, vacuum from 8.8 to 12.8psi @20:38:26.67 SS.reconfigure SSinhale.with maxSpeed: 53 @20:38:26.74 SS.seek 25ml @20:38:32.63 Vacuum too high -- waiting up to 40s @20:38:38.01 Intake @15.4psia, vacuum from 8.9 to 12.9psi @20:38:38.03 SS.reconfigure SSinhale.with maxSpeed: 48 @20:38:38.10 SS.seek 25ml @20:38:45.10 Vacuum too high -- waiting up to 40s @20:38:50.94 Intake @15.4psia, vacuum from 8.9 to 12.9psi @20:38:50.96 SS.reconfigure SSinhale.with maxSpeed: 43 @20:38:51.03 SS.seek 25ml @20:38:59.28 delay 20 seconds @20:39:01.54 Vacuum too high -- waiting up to 40s @20:39:09.94 Intake @15.4psia, vacuum from 8.9 to 12.9psi @20:39:09.96 SS.reconfigure SSinhale.with maxSpeed: 39 @20:39:10.04 SS.seek 25ml @20:39:19.31 AS.seek 0.07ml @20:39:19.44 Stroke completed -- waiting for pressure to equalize ... @20:39:30.49 Intake.select :close @20:39:30.64 SSV.dial :exhaust @20:39:31.53 Exhaust.select :open @20:39:31.66 SS.reconfigure SSexhale @20:39:31.72 SS.seek 0ml @20:39:40.95 Exhaust.select :close @20:39:41.56 SSV.dial CBV @20:39:41.59 CBV.dial SSV @20:39:42.50 Allowing 130s for 25.0ml of remaining 1425.0ml @20:39:42.52 Intake.select :open @20:39:44.70 Intake @15.5psia, vacuum from 9.0 to 13.0psi @20:39:44.72 SS.reconfigure SSinhale.with maxSpeed: 39 @20:39:44.80 SS.seek 25ml @20:39:57.23 Vacuum too high -- waiting up to 40s @20:40:03.53 Intake @15.3psia, vacuum from 8.8 to 12.8psi @20:40:03.55 SS.reconfigure SSinhale.with maxSpeed: 35 @20:40:03.62 SS.seek 25ml @20:40:05.83 delay 20 seconds @20:40:17.13 Vacuum too high -- waiting up to 40s @20:40:24.62 Intake @15.4psia, vacuum from 8.9 to 12.9psi @20:40:24.64 SS.reconfigure SSinhale.with maxSpeed: 32 @20:40:24.72 SS.seek 25ml @20:40:25.85 ATV.dial :waste2 @20:40:26.64 AS.reconfigure ASconfig @20:40:26.70 AS.seek :empty @20:40:31.09 Elution @20:40:31.10 ATV.dial :air1 @20:40:32.31 AS.reconfigure ASslow1 @20:40:32.37 AS.seek 0.049ml @20:40:42.80 delay 3 seconds @20:40:45.10 Vacuum too high -- waiting up to 40s @20:40:45.82 ATV.dial :ElutionWater @20:40:46.57 AS.seek 0.109ml @20:40:54.17 Intake @15.4psia, vacuum from 8.9 to 12.9psi @20:40:54.19 SS.reconfigure SSinhale.with maxSpeed: 29 @20:40:54.26 SS.seek 25ml @20:40:58.98 delay 3 seconds @20:41:01.39 Stroke completed -- waiting for pressure to equalize ... @20:41:02.49 ATV.dial ASV,via: :air2 @20:41:04.74 ASV.dial :SPEelution @20:41:05.33 AFV.select :carrierSolution @20:41:05.50 AS.reconfigure ASnorm @20:41:05.56 AS.seek 0.759ml @20:41:09.44 Intake.select :close @20:41:09.60 SSV.dial :exhaust @20:41:10.48 Exhaust.select :open @20:41:10.61 SS.reconfigure SSexhale @20:41:10.67 SS.seek 0ml @20:41:20.21 Exhaust.select :close @20:41:20.32 SSV.dial CBV @20:41:20.35 CBV.dial SSV @20:41:21.23 Allowing 130s for 25.0ml of remaining 1400.0ml @20:41:21.24 Intake.select :open @20:41:23.44 Intake @15.5psia, vacuum from 9.0 to 13.0psi @20:41:23.45 SS.reconfigure SSinhale.with maxSpeed: 29 @20:41:23.53 SS.seek 25ml @20:41:38.63 delay 3 seconds @20:41:41.65 AFV.select :holdingCoil @20:41:41.80 AS.reconfigure ASslow1 @20:41:41.86 AS.seek 0.474ml @20:41:47.54 Vacuum too high -- waiting up to 40s @20:41:54.30 Intake @15.3psia, vacuum from 8.8 to 12.8psi @20:41:54.32 SS.reconfigure SSinhale.with maxSpeed: 26 @20:41:54.39 SS.seek 25ml @20:42:36.39 Stroke completed -- waiting for pressure to equalize ... @20:42:39.47 delay 5 seconds @20:42:44.55 AS.reconfigure ASslow2 @20:42:44.61 AS.seek 0.439ml @20:42:49.91 Intake.select :close @20:42:50.07 SSV.dial :exhaust @20:42:50.95 Exhaust.select :open @20:42:51.08 SS.reconfigure SSexhale @20:42:51.14 SS.seek 0ml @20:43:00.61 Exhaust.select :close @20:43:00.73 SSV.dial CBV @20:43:00.78 CBV.dial SSV @20:43:01.70 Allowing 130s for 25.0ml of remaining 1375.0ml @20:43:01.71 Intake.select :open @20:43:03.91 Intake @15.5psia, vacuum from 9.0 to 13.0psi @20:43:03.92 SS.reconfigure SSinhale.with maxSpeed: 26 @20:43:04.00 SS.seek 25ml @20:43:19.98 delay 1:00 @20:43:40.47 Vacuum too high -- waiting up to 40s @20:43:48.18 Intake @15.3psia, vacuum from 8.8 to 12.8psi @20:43:48.20 SS.reconfigure SSinhale.with maxSpeed: 23 @20:43:48.27 SS.seek 25ml @20:44:20.01 pushing eluate to holding coil @20:44:20.10 AS.seek 0.279ml @20:44:24.33 Stroke completed -- waiting for pressure to equalize ... @20:44:34.78 Intake.select :close @20:44:34.94 SSV.dial :exhaust @20:44:35.82 Exhaust.select :open @20:44:35.95 SS.reconfigure SSexhale @20:44:36.02 SS.seek 0ml @20:44:45.85 Exhaust.select :close @20:44:45.97 SSV.dial CBV @20:44:46.00 CBV.dial SSV @20:44:46.88 Allowing 130s for 25.0ml of remaining 1350.0ml @20:44:46.89 Intake.select :open @20:44:49.08 Intake @15.5psia, vacuum from 9.0 to 13.0psi @20:44:49.11 SS.reconfigure SSinhale.with maxSpeed: 23 @20:44:49.18 SS.seek 25ml @20:46:06.03 Stroke completed -- waiting for pressure to equalize ... @20:46:19.36 Intake.select :close @20:46:19.52 SSV.dial :exhaust @20:46:20.40 Exhaust.select :open @20:46:20.53 SS.reconfigure SSexhale @20:46:20.59 SS.seek 0ml @20:46:30.21 Exhaust.select :close @20:46:30.32 SSV.dial CBV @20:46:30.35 CBV.dial SSV @20:46:31.26 Allowing 130s for 25.0ml of remaining 1325.0ml @20:46:31.28 Intake.select :open @20:46:33.46 Intake @15.5psia, vacuum from 9.0 to 13.0psi @20:46:33.47 SS.reconfigure SSinhale.with maxSpeed: 23 @20:46:33.55 SS.seek 25ml @20:47:01.31 delay 30 seconds @20:47:31.34 ATV.dial :waste,via: :air3 @20:47:33.19 AS.reconfigure ASconfig @20:47:33.25 AS.seek :empty @20:47:49.67 Parking Bleach in LysateSource line and SPEcolumn @20:47:49.68 ATV.dial :air3 @20:47:50.58 AS.reconfigure ASslow1 @20:47:50.66 AS.seek 0.029ml @20:47:50.68 Stroke completed -- waiting for pressure to equalize ... @20:47:57.08 ATV.dial :ElutionWater @20:47:57.73 AS.seek 0.059ml @20:48:04.07 ATV.dial :air3 @20:48:04.76 AS.seek 0.069ml @20:48:04.97 Intake.select :close @20:48:05.13 SSV.dial :exhaust @20:48:06.02 Exhaust.select :open @20:48:06.15 SS.reconfigure SSexhale @20:48:06.21 SS.seek 0ml @20:48:07.11 delay 2 seconds @20:48:09.13 ATV.dial :bleach @20:48:09.61 AS.seek 0.769ml @20:48:16.05 Exhaust.select :close @20:48:16.18 SSV.dial CBV @20:48:16.21 CBV.dial SSV @20:48:17.10 Allowing 130s for 25.0ml of remaining 1300.0ml @20:48:17.12 Intake.select :open @20:48:19.31 Intake @15.5psia, vacuum from 9.0 to 13.0psi @20:48:19.32 SS.reconfigure SSinhale.with maxSpeed: 23 @20:48:19.40 SS.seek 25ml @20:49:36.15 Stroke completed -- waiting for pressure to equalize ... @20:49:50.12 Intake.select :close @20:49:50.28 SSV.dial :exhaust @20:49:51.15 Exhaust.select :open @20:49:51.28 SS.reconfigure SSexhale @20:49:51.34 SS.seek 0ml @20:50:00.92 Exhaust.select :close @20:50:01.05 SSV.dial CBV @20:50:01.08 CBV.dial SSV @20:50:01.98 Allowing 130s for 25.0ml of remaining 1275.0ml @20:50:01.99 Intake.select :open @20:50:04.18 Intake @15.5psia, vacuum from 9.0 to 13.0psi @20:50:04.21 SS.reconfigure SSinhale.with maxSpeed: 23 @20:50:04.28 SS.seek 25ml @20:50:30.64 delay 3 seconds @20:50:33.66 ATV.dial :air3 @20:50:34.23 delay 3 seconds @20:50:37.30 AS.seek 0.794ml @20:50:42.64 delay 3 seconds @20:50:45.67 ATV.dial :bleach @20:50:46.33 AS.seek 0.779ml @20:50:49.64 delay 3 seconds @20:50:52.67 ATV.dial :AIV @20:50:54.02 AS.reconfigure ASconfig @20:50:54.08 AS.seek 0.015ml @20:51:21.62 Stroke completed -- waiting for pressure to equalize ... @20:51:37.98 Intake.select :close @20:51:38.15 SSV.dial :exhaust @20:51:38.30 ATV.dial :waste @20:51:39.04 Exhaust.select :open @20:51:39.17 SS.reconfigure SSexhale @20:51:39.23 SS.seek 0ml @20:51:39.89 AS.seek :empty @20:51:41.09 ATV.dial :air3 @20:51:41.81 AS.reconfigure ASslow1 @20:51:41.87 AS.seek 0.009ml @20:51:44.22 delay 2 seconds @20:51:46.24 ATV.dial :bleach @20:51:46.72 AS.seek 0.579ml @20:51:49.09 Exhaust.select :close @20:51:49.22 SSV.dial CBV @20:51:49.25 CBV.dial SSV @20:51:50.15 Allowing 130s for 25.0ml of remaining 1250.0ml @20:51:50.16 Intake.select :open @20:51:52.35 Intake @15.5psia, vacuum from 9.0 to 13.0psi @20:51:52.38 SS.reconfigure SSinhale.with maxSpeed: 23 @20:51:52.45 SS.seek 25ml @20:52:55.94 Vacuum too high -- waiting up to 40s @20:53:05.26 Intake @15.4psia, vacuum from 8.9 to 12.9psi @20:53:05.28 SS.reconfigure SSinhale.with maxSpeed: 21 @20:53:05.35 SS.seek 25ml @20:53:20.67 Stroke completed -- waiting for pressure to equalize ... @20:53:28.68 Intake.select :close @20:53:28.84 SSV.dial :exhaust @20:53:29.73 Exhaust.select :open @20:53:29.86 SS.reconfigure SSexhale @20:53:29.92 SS.seek 0ml @20:53:39.76 Exhaust.select :close @20:53:39.87 SSV.dial CBV @20:53:39.90 CBV.dial SSV @20:53:40.80 Allowing 130s for 25.0ml of remaining 1225.0ml @20:53:40.81 Intake.select :open @20:53:41.61 delay 3 seconds @20:53:43.01 Intake @15.5psia, vacuum from 9.0 to 13.0psi @20:53:43.02 SS.reconfigure SSinhale.with maxSpeed: 21 @20:53:43.10 SS.seek 25ml @20:53:44.63 ATV.dial :air3 @20:53:45.23 delay 3 seconds @20:53:48.30 AS.seek 0.604ml @20:53:53.63 delay 3 seconds @20:53:56.65 ATV.dial :bleach @20:53:57.29 AS.seek 0.589ml @20:54:00.63 delay 3 seconds @20:54:03.65 ATV.dial :ASV @20:54:04.75 ASV.dial :wasteR @20:54:05.35 AS.seek 0ml @20:55:07.70 Stroke completed -- waiting for pressure to equalize ... @20:55:20.57 Intake.select :close @20:55:20.74 SSV.dial :exhaust @20:55:21.62 Exhaust.select :open @20:55:21.75 SS.reconfigure SSexhale @20:55:21.81 SS.seek 0ml @20:55:31.37 Exhaust.select :close @20:55:31.99 SSV.dial CBV @20:55:32.02 CBV.dial SSV @20:55:32.91 Allowing 130s for 25.0ml of remaining 1200.0ml @20:55:32.93 Intake.select :open @20:55:35.12 Intake @15.5psia, vacuum from 9.0 to 13.0psi @20:55:35.14 SS.reconfigure SSinhale.with maxSpeed: 21 @20:55:35.22 SS.seek 25ml @20:56:04.11 delay 1:00 @20:56:59.92 Stroke completed -- waiting for pressure to equalize ... @20:57:04.19 AFV.select :carrierSolution @20:57:04.30 delay 2 seconds @20:57:06.32 AS.reconfigure ASconfig @20:57:06.39 AS.seek 1ml @20:57:14.08 Intake.select :close @20:57:14.24 SSV.dial :exhaust @20:57:15.11 Exhaust.select :open @20:57:15.24 SS.reconfigure SSexhale @20:57:15.30 SS.seek 0ml @20:57:24.97 Exhaust.select :close @20:57:25.09 SSV.dial CBV @20:57:25.12 CBV.dial SSV @20:57:26.02 Allowing 130s for 25.0ml of remaining 1175.0ml @20:57:26.03 Intake.select :open @20:57:28.23 Intake @15.5psia, vacuum from 9.0 to 13.0psi @20:57:28.24 SS.reconfigure SSinhale.with maxSpeed: 21 @20:57:28.32 SS.seek 25ml @20:58:04.17 delay 1 second @20:58:05.19 AFV.select :holdingCoil @20:58:05.30 delay 1 second @20:58:06.32 ATV.dial ASV @20:58:06.33 ASV.dial :wasteR @20:58:06.36 AS.reconfigure ASslow1 @20:58:06.43 AS.seek 0ml @20:58:52.67 Stroke completed -- waiting for pressure to equalize ... @20:59:06.03 Intake.select :close @20:59:06.19 SSV.dial :exhaust @20:59:07.08 Exhaust.select :open @20:59:07.21 SS.reconfigure SSexhale @20:59:07.27 SS.seek 0ml @20:59:16.83 Exhaust.select :close @20:59:16.95 SSV.dial CBV @20:59:16.98 CBV.dial SSV @20:59:17.86 Allowing 130s for 25.0ml of remaining 1150.0ml @20:59:17.88 Intake.select :open @20:59:20.07 Intake @15.6psia, vacuum from 9.1 to 13.1psi @20:59:20.08 SS.reconfigure SSinhale.with maxSpeed: 21 @20:59:20.16 SS.seek 25ml @21:00:44.76 Stroke completed -- waiting for pressure to equalize ... @21:00:58.86 Intake.select :close @21:00:59.03 SSV.dial :exhaust @21:00:59.91 Exhaust.select :open @21:01:00.04 SS.reconfigure SSexhale @21:01:00.10 SS.seek 0ml @21:01:09.93 Exhaust.select :close @21:01:10.06 SSV.dial CBV @21:01:10.09 CBV.dial SSV @21:01:10.99 Allowing 130s for 25.0ml of remaining 1125.0ml @21:01:11.00 Intake.select :open @21:01:13.20 Intake @15.6psia, vacuum from 9.1 to 13.1psi @21:01:13.21 SS.reconfigure SSinhale.with maxSpeed: 21 @21:01:13.29 SS.seek 25ml @21:01:27.83 delay 20 seconds @21:01:47.86 ATV.dial :air3 @21:01:49.47 AS.seek 0.59ml @21:02:38.20 Stroke completed -- waiting for pressure to equalize ... @21:02:52.18 Intake.select :close @21:02:52.34 SSV.dial :exhaust @21:02:53.22 Exhaust.select :open @21:02:53.35 SS.reconfigure SSexhale @21:02:53.41 SS.seek 0ml @21:03:02.99 Exhaust.select :close @21:03:03.12 SSV.dial CBV @21:03:03.15 CBV.dial SSV @21:03:04.04 Allowing 130s for 25.0ml of remaining 1100.0ml @21:03:04.05 Intake.select :open @21:03:06.25 Intake @15.6psia, vacuum from 9.1 to 13.1psi @21:03:06.26 SS.reconfigure SSinhale.with maxSpeed: 21 @21:03:06.34 SS.seek 25ml @21:03:48.42 delay 3 seconds @21:03:51.44 ATV.dial :ASV @21:03:52.95 ASV.dial :wasteR @21:03:53.04 AS.seek 0.09ml @21:04:31.04 Stroke completed -- waiting for pressure to equalize ... @21:04:46.06 Intake.select :close @21:04:46.22 SSV.dial :exhaust @21:04:47.09 Exhaust.select :open @21:04:47.22 SS.reconfigure SSexhale @21:04:47.28 SS.seek 0ml @21:04:57.01 Exhaust.select :close @21:04:57.14 SSV.dial CBV @21:04:57.17 CBV.dial SSV @21:04:58.07 Allowing 130s for 25.0ml of remaining 1075.0ml @21:04:58.08 Intake.select :open @21:05:00.76 Intake @15.5psia, vacuum from 9.0 to 13.0psi @21:05:00.78 SS.reconfigure SSinhale.with maxSpeed: 21 @21:05:00.85 SS.seek 25ml @21:05:33.91 delay 30 seconds @21:06:03.94 ATV.dial :extSrc @21:06:04.75 delay 3 seconds @21:06:07.77 ATV.dial :ElutionWater @21:06:08.98 AS.seek 0.12ml @21:06:15.33 ATV.dial :air3 @21:06:15.97 AS.seek 0.13ml @21:06:18.27 delay 2 seconds @21:06:20.29 ATV.dial :bleach @21:06:20.79 AS.seek 0.23ml @21:06:25.58 Stroke completed -- waiting for pressure to equalize ... @21:06:39.70 Intake.select :close @21:06:39.84 SSV.dial :exhaust @21:06:40.72 Exhaust.select :open @21:06:40.85 SS.reconfigure SSexhale @21:06:40.91 SS.seek 0ml @21:06:41.20 delay 3 seconds @21:06:44.22 ATV.dial :air3 @21:06:44.81 delay 3 seconds @21:06:47.88 AS.seek 0.255ml @21:06:50.66 Exhaust.select :close @21:06:50.77 SSV.dial CBV @21:06:50.80 CBV.dial SSV @21:06:51.69 Allowing 130s for 25.0ml of remaining 1050.0ml @21:06:51.70 Intake.select :open @21:06:53.17 delay 3 seconds @21:06:53.90 Intake @15.5psia, vacuum from 9.0 to 13.0psi @21:06:53.91 SS.reconfigure SSinhale.with maxSpeed: 21 @21:06:54.00 SS.seek 25ml @21:06:56.19 ATV.dial :bleach @21:06:56.84 AS.seek 0.24ml @21:07:00.16 delay 3 seconds @21:07:03.18 ATV.dial :ASV @21:07:04.28 ASV.dial :SPEdelivery @21:07:04.67 AFV.select :carrierSolution @21:07:04.78 delay 2 seconds @21:07:06.80 AS.reconfigure ASconfig @21:07:06.86 AS.seek 0.5ml @21:07:22.11 delay 1 second @21:07:23.13 AFV.select :holdingCoil @21:07:23.29 AS.reconfigure ASslow1 @21:07:23.35 AS.seek 0.17ml @21:08:18.71 Stroke completed -- waiting for pressure to equalize ... @21:08:30.01 SPE.hold 2:46:40,95C @21:08:30.08 delay 3:00 @21:08:32.72 Intake.select :close @21:08:32.88 SSV.dial :exhaust @21:08:33.80 Exhaust.select :open @21:08:33.93 SS.reconfigure SSexhale @21:08:33.99 SS.seek 0ml @21:08:43.75 Exhaust.select :close @21:08:43.86 SSV.dial CBV @21:08:43.89 CBV.dial SSV @21:08:44.83 Allowing 130s for 25.0ml of remaining 1025.0ml @21:08:44.84 Intake.select :open @21:08:47.04 Intake @15.6psia, vacuum from 9.1 to 13.1psi @21:08:47.05 SS.reconfigure SSinhale.with maxSpeed: 21 @21:08:47.13 SS.seek 25ml @21:10:11.65 Stroke completed -- waiting for pressure to equalize ... @21:10:26.37 Intake.select :close @21:10:26.51 SSV.dial :exhaust @21:10:27.42 Exhaust.select :open @21:10:27.55 SS.reconfigure SSexhale @21:10:27.61 SS.seek 0ml @21:10:37.39 Exhaust.select :close @21:10:37.51 SSV.dial CBV @21:10:37.54 CBV.dial SSV @21:10:38.95 Allowing 130s for 25.0ml of remaining 1000.0ml @21:10:38.97 Intake.select :open @21:10:41.15 Intake @15.6psia, vacuum from 9.1 to 13.1psi @21:10:41.16 SS.reconfigure SSinhale.with maxSpeed: 21 @21:10:41.25 SS.seek 25ml @21:11:30.11 AFV.select :carrierSolution @21:11:30.22 delay 2 seconds @21:11:32.24 AS.reconfigure ASconfig @21:11:32.30 AS.seek 1ml @21:12:05.81 Stroke completed -- waiting for pressure to equalize ... @21:12:20.31 delay 1 second @21:12:20.51 Intake.select :close @21:12:20.67 SSV.dial :exhaust @21:12:21.33 AFV.select :holdingCoil @21:12:21.44 delay 1 second @21:12:21.56 Exhaust.select :open @21:12:21.69 SS.reconfigure SSexhale @21:12:21.75 SS.seek 0ml @21:12:22.46 ASV.dial :wasteD @21:12:23.55 AS.seek 0ml @21:12:31.49 Exhaust.select :close @21:12:31.60 SSV.dial CBV @21:12:31.63 CBV.dial SSV @21:12:32.55 Allowing 130s for 25.0ml of remaining 975.0ml @21:12:32.57 Intake.select :open @21:12:34.76 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:12:34.78 SS.reconfigure SSinhale.with maxSpeed: 21 @21:12:34.85 SS.seek 25ml @21:13:21.34 Priming Eluate @21:13:21.35 AFV.select :carrierSolution @21:13:21.48 delay 2 seconds @21:13:23.51 AS.seek 0.5ml @21:13:52.53 delay 1 second @21:13:53.55 AFV.select :holdingCoil @21:13:53.65 ATV.dial ASV @21:13:53.66 ASV.dial :SPErecovery @21:13:54.31 Swish #1 of 2 @21:13:54.40 AS.reconfigure ASslow0 @21:13:54.45 AS.seek 0.6ml @21:13:59.46 Stroke completed -- waiting for pressure to equalize ... @21:14:04.99 AS.seek 0.5ml @21:14:15.41 Intake.select :close @21:14:15.43 Swish #2 of 2 @21:14:15.55 AS.seek 0.6ml @21:14:15.67 SSV.dial :exhaust @21:14:16.56 Exhaust.select :open @21:14:16.69 SS.reconfigure SSexhale @21:14:16.75 SS.seek 0ml @21:14:26.04 AS.seek 0.5ml @21:14:26.44 Exhaust.select :close @21:14:26.55 SSV.dial CBV @21:14:26.58 CBV.dial SSV @21:14:27.48 Allowing 130s for 25.0ml of remaining 950.0ml @21:14:27.49 Intake.select :open @21:14:29.69 Intake @15.6psia, vacuum from 9.1 to 13.1psi @21:14:29.71 SS.reconfigure SSinhale.with maxSpeed: 21 @21:14:29.78 SS.seek 25ml @21:14:36.41 delay 3 seconds @21:14:39.48 AS.reconfigure ASslow1 @21:14:39.54 AS.seek 0.684ml @21:15:16.94 delay 3 seconds @21:15:19.96 Eluate primed @21:15:19.97 ATV.dial :waste2 @21:15:20.67 AS.reconfigure ASnorm @21:15:20.73 AS.seek 0ml @21:15:54.48 Stroke completed -- waiting for pressure to equalize ... @21:15:55.52 SPE.coast @21:15:55.61 ASV.dial :SPErecovery @21:15:55.63 Setting up PCR for IPc -- writing /var/log/esp/IPc19may1621h1500ml.pcr @21:15:55.65 Building PCR Slug from ASV with MM1 mix, PP1 primer @21:15:55.67 Priming with carrier @21:15:55.69 ATV.dial ARV @21:15:56.73 ARV.dial :carrier @21:15:57.14 AS.seek 0.5ml @21:16:10.62 Intake.select :close @21:16:10.78 SSV.dial :exhaust @21:16:11.67 Exhaust.select :open @21:16:11.80 SS.reconfigure SSexhale @21:16:11.86 SS.seek 0ml @21:16:21.80 Exhaust.select :close @21:16:21.91 SSV.dial CBV @21:16:21.94 CBV.dial SSV @21:16:22.84 Allowing 130s for 25.0ml of remaining 925.0ml @21:16:22.86 Intake.select :open @21:16:22.94 AFV.select :carrierSolution @21:16:23.05 delay 2 seconds @21:16:25.07 Intake @15.6psia, vacuum from 9.1 to 13.1psi @21:16:25.08 SS.reconfigure SSinhale.with maxSpeed: 21 @21:16:25.16 SS.seek 25ml @21:16:25.23 AS.reconfigure ASconfig @21:16:25.29 AS.seek 1ml @21:16:54.31 delay 1 second @21:16:55.33 AFV.select :holdingCoil @21:16:55.44 delay 1 second @21:16:56.46 ATV.dial :waste @21:16:58.05 AS.reconfigure ASnorm @21:16:58.12 AS.seek :empty @21:17:48.87 AFV.select :carrierSolution @21:17:48.99 delay 2 seconds @21:17:49.57 Stroke completed -- waiting for pressure to equalize ... @21:17:51.01 AS.reconfigure ASconfig @21:17:51.08 AS.seek 0.5ml @21:18:04.28 Intake.select :close @21:18:04.45 SSV.dial :exhaust @21:18:05.33 Exhaust.select :open @21:18:05.46 SS.reconfigure SSexhale @21:18:05.52 SS.seek 0ml @21:18:15.16 Exhaust.select :close @21:18:15.28 SSV.dial CBV @21:18:15.31 CBV.dial SSV @21:18:16.19 Allowing 130s for 25.0ml of remaining 900.0ml @21:18:16.20 Intake.select :open @21:18:18.39 Intake @15.6psia, vacuum from 9.1 to 13.1psi @21:18:18.42 SS.reconfigure SSinhale.with maxSpeed: 21 @21:18:18.49 SS.seek 25ml @21:18:20.20 delay 1 second @21:18:21.22 AFV.select :holdingCoil @21:18:21.32 Building Slug @21:18:21.33 ATV.dial :ARV @21:18:23.06 delay 2 seconds @21:18:25.57 ARV.dial :air1 @21:18:25.98 delay 2 seconds @21:18:28.05 AS.reconfigure ASslow2 @21:18:28.11 AS.seek 0.506ml @21:18:34.30 delay 2 seconds @21:18:36.32 ARV.dial :MM1 @21:18:36.94 delay 2 seconds @21:18:38.96 Adding MM1 @21:18:39.04 AS.seek 0.524ml @21:18:57.37 delay 25 seconds @21:19:22.39 ARV.dial :PP1 @21:19:22.80 Adding PP1 @21:19:22.82 delay 2 seconds @21:19:24.91 AS.seek 0.53ml @21:19:31.28 delay 10 seconds @21:19:41.31 ARV.dial :air1 @21:19:41.72 adding ARV air @21:19:41.73 delay 2 seconds @21:19:43.04 Stroke completed -- waiting for pressure to equalize ... @21:19:43.82 AS.seek 0.536ml @21:19:50.05 delay 5 seconds @21:19:55.07 ARV.dial :carrier @21:19:55.47 delay 7 seconds @21:19:59.60 Intake.select :close @21:19:59.76 SSV.dial :exhaust @21:20:00.64 Exhaust.select :open @21:20:00.77 SS.reconfigure SSexhale @21:20:00.83 SS.seek 0ml @21:20:02.54 AS.seek 0.594ml @21:20:10.45 Exhaust.select :close @21:20:10.56 SSV.dial CBV @21:20:10.59 CBV.dial SSV @21:20:11.48 Allowing 130s for 25.0ml of remaining 875.0ml @21:20:11.50 Intake.select :open @21:20:13.68 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:20:13.71 SS.reconfigure SSinhale.with maxSpeed: 21 @21:20:13.78 SS.seek 25ml @21:21:01.03 delay 5 seconds @21:21:06.05 ATV.dial :ASV @21:21:06.48 delay 2 seconds @21:21:08.55 AS.seek 0.6ml @21:21:15.01 delay 4 seconds @21:21:19.03 ATV.dial ARV @21:21:19.59 delay 2 seconds @21:21:21.61 ARV.dial :carrier @21:21:22.06 delay 2 seconds @21:21:24.21 AS.seek 0.65ml @21:21:38.38 Stroke completed -- waiting for pressure to equalize ... @21:21:53.72 Intake.select :close @21:21:53.86 SSV.dial :exhaust @21:21:54.76 Exhaust.select :open @21:21:54.90 SS.reconfigure SSexhale @21:21:54.96 SS.seek 0ml @21:22:04.75 Exhaust.select :close @21:22:04.86 SSV.dial CBV @21:22:04.89 CBV.dial SSV @21:22:05.78 Allowing 130s for 25.0ml of remaining 850.0ml @21:22:05.79 Intake.select :open @21:22:07.99 Intake @15.6psia, vacuum from 9.1 to 13.1psi @21:22:08.00 SS.reconfigure SSinhale.with maxSpeed: 21 @21:22:08.08 SS.seek 25ml @21:22:14.78 delay 4 seconds @21:22:18.80 ATV.dial PCR @21:22:19.45 ASV.dial :waste1 @21:22:20.25 ARV.dial :air1 @21:22:20.67 Positioning Slug by 0.324ml @21:22:20.76 AS.seek 0.326ml @21:23:32.58 Stroke completed -- waiting for pressure to equalize ... @21:23:48.43 Intake.select :close @21:23:48.59 SSV.dial :exhaust @21:23:49.47 Exhaust.select :open @21:23:49.60 SS.reconfigure SSexhale @21:23:49.66 SS.seek 0ml @21:23:59.20 Exhaust.select :close @21:23:59.31 SSV.dial CBV @21:23:59.34 CBV.dial SSV @21:24:00.22 Allowing 130s for 25.0ml of remaining 825.0ml @21:24:00.23 Intake.select :open @21:24:02.43 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:24:02.44 SS.reconfigure SSinhale.with maxSpeed: 21 @21:24:02.52 SS.seek 25ml @21:25:27.27 Stroke completed -- waiting for pressure to equalize ... @21:25:44.43 Intake.select :close @21:25:44.58 SSV.dial :exhaust @21:25:45.47 Exhaust.select :open @21:25:45.60 SS.reconfigure SSexhale @21:25:45.66 SS.seek 0ml @21:25:55.56 Exhaust.select :close @21:25:55.68 SSV.dial CBV @21:25:55.71 CBV.dial SSV @21:25:56.61 Allowing 130s for 25.0ml of remaining 800.0ml @21:25:56.62 Intake.select :open @21:25:58.81 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:25:58.84 SS.reconfigure SSinhale.with maxSpeed: 21 @21:25:58.91 SS.seek 25ml @21:27:10.43 Vacuum too high -- waiting up to 40s @21:27:19.10 Intake @15.4psia, vacuum from 8.9 to 12.9psi @21:27:19.12 SS.reconfigure SSinhale.with maxSpeed: 19 @21:27:19.19 SS.seek 25ml @21:27:33.75 Stroke completed -- waiting for pressure to equalize ... @21:27:42.36 Intake.select :close @21:27:42.52 SSV.dial :exhaust @21:27:43.40 Exhaust.select :open @21:27:43.53 SS.reconfigure SSexhale @21:27:43.59 SS.seek 0ml @21:27:46.83 delay 5 seconds @21:27:51.85 ATV.dial ARV @21:27:52.66 ASV.dial :PCRlock @21:27:53.06 delay 5 seconds @21:27:53.16 Exhaust.select :close @21:27:53.29 SSV.dial CBV @21:27:53.85 CBV.dial SSV @21:27:54.72 Allowing 130s for 25.0ml of remaining 775.0ml @21:27:54.74 Intake.select :open @21:27:56.94 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:27:56.96 SS.reconfigure SSinhale.with maxSpeed: 19 @21:27:57.03 SS.seek 25ml @21:27:58.08 Running IPc ProfileIPc thermocycling @21:27:58.10 PCR.program ProfileIPc @21:27:58.26 ProfileIPc should complete in < 1:50:49 @21:27:58.27 PCR.run 2:23:31.25 @21:29:30.86 Stroke completed -- waiting for pressure to equalize ... @21:29:44.36 Intake.select :close @21:29:44.53 SSV.dial :exhaust @21:29:45.40 Exhaust.select :open @21:29:45.53 SS.reconfigure SSexhale @21:29:45.59 SS.seek 0ml @21:29:55.39 Exhaust.select :close @21:29:55.52 SSV.dial CBV @21:29:55.55 CBV.dial SSV @21:29:56.43 Allowing 130s for 25.0ml of remaining 750.0ml @21:29:56.45 Intake.select :open @21:29:58.64 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:29:58.65 SS.reconfigure SSinhale.with maxSpeed: 19 @21:29:58.73 SS.seek 25ml @21:31:32.45 Stroke completed -- waiting for pressure to equalize ... @21:31:45.17 Intake.select :close @21:31:45.32 SSV.dial :exhaust @21:31:46.18 Exhaust.select :open @21:31:46.31 SS.reconfigure SSexhale @21:31:46.37 SS.seek 0ml @21:31:55.95 Exhaust.select :close @21:31:56.06 SSV.dial CBV @21:31:56.09 CBV.dial SSV @21:31:56.96 Allowing 130s for 25.0ml of remaining 725.0ml @21:31:56.98 Intake.select :open @21:31:59.16 Intake @15.6psia, vacuum from 9.1 to 13.1psi @21:31:59.17 SS.reconfigure SSinhale.with maxSpeed: 19 @21:31:59.26 SS.seek 25ml @21:33:33.19 Stroke completed -- waiting for pressure to equalize ... @21:33:46.09 Intake.select :close @21:33:46.25 SSV.dial :exhaust @21:33:47.12 Exhaust.select :open @21:33:47.25 SS.reconfigure SSexhale @21:33:47.31 SS.seek 0ml @21:33:57.06 Exhaust.select :close @21:33:57.19 SSV.dial CBV @21:33:57.22 CBV.dial SSV @21:33:58.10 Allowing 130s for 25.0ml of remaining 700.0ml @21:33:58.11 Intake.select :open @21:34:00.30 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:34:00.31 SS.reconfigure SSinhale.with maxSpeed: 19 @21:34:00.39 SS.seek 25ml @21:35:34.09 Stroke completed -- waiting for pressure to equalize ... @21:35:46.97 Intake.select :close @21:35:47.13 SSV.dial :exhaust @21:35:48.01 Exhaust.select :open @21:35:48.14 SS.reconfigure SSexhale @21:35:48.20 SS.seek 0ml @21:35:58.06 Exhaust.select :close @21:35:58.19 SSV.dial CBV @21:35:58.22 CBV.dial SSV @21:35:59.12 Allowing 130s for 25.0ml of remaining 675.0ml @21:35:59.14 Intake.select :open @21:36:01.33 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:36:01.34 SS.reconfigure SSinhale.with maxSpeed: 19 @21:36:01.42 SS.seek 25ml @21:37:35.05 Stroke completed -- waiting for pressure to equalize ... @21:37:47.86 Intake.select :close @21:37:48.02 SSV.dial :exhaust @21:37:48.90 Exhaust.select :open @21:37:49.03 SS.reconfigure SSexhale @21:37:49.09 SS.seek 0ml @21:37:58.93 Exhaust.select :close @21:37:59.04 SSV.dial CBV @21:37:59.07 CBV.dial SSV @21:37:59.96 Allowing 130s for 25.0ml of remaining 650.0ml @21:37:59.98 Intake.select :open @21:38:02.17 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:38:02.18 SS.reconfigure SSinhale.with maxSpeed: 19 @21:38:02.26 SS.seek 25ml @21:39:35.97 Stroke completed -- waiting for pressure to equalize ... @21:39:48.88 Intake.select :close @21:39:49.04 SSV.dial :exhaust @21:39:49.93 Exhaust.select :open @21:39:50.06 SS.reconfigure SSexhale @21:39:50.12 SS.seek 0ml @21:39:59.81 Exhaust.select :close @21:39:59.93 SSV.dial CBV @21:39:59.96 CBV.dial SSV @21:40:00.87 Allowing 130s for 25.0ml of remaining 625.0ml @21:40:00.88 Intake.select :open @21:40:03.08 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:40:03.10 SS.reconfigure SSinhale.with maxSpeed: 19 @21:40:03.17 SS.seek 25ml @21:41:37.08 Stroke completed -- waiting for pressure to equalize ... @21:41:50.49 Intake.select :close @21:41:50.65 SSV.dial :exhaust @21:41:51.54 Exhaust.select :open @21:41:51.69 SS.reconfigure SSexhale @21:41:51.75 SS.seek 0ml @21:42:01.40 Exhaust.select :close @21:42:01.51 SSV.dial CBV @21:42:01.54 CBV.dial SSV @21:42:02.43 Allowing 130s for 25.0ml of remaining 600.0ml @21:42:02.45 Intake.select :open @21:42:04.64 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:42:04.65 SS.reconfigure SSinhale.with maxSpeed: 19 @21:42:04.73 SS.seek 25ml @21:43:38.50 Stroke completed -- waiting for pressure to equalize ... @21:43:52.00 Intake.select :close @21:43:52.16 SSV.dial :exhaust @21:43:53.04 Exhaust.select :open @21:43:53.17 SS.reconfigure SSexhale @21:43:53.23 SS.seek 0ml @21:44:02.87 Exhaust.select :close @21:44:02.99 SSV.dial CBV @21:44:03.02 CBV.dial SSV @21:44:03.91 Allowing 130s for 25.0ml of remaining 575.0ml @21:44:03.93 Intake.select :open @21:44:06.12 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:44:06.13 SS.reconfigure SSinhale.with maxSpeed: 19 @21:44:06.21 SS.seek 25ml @21:45:40.12 Stroke completed -- waiting for pressure to equalize ... @21:45:53.02 Intake.select :close @21:45:53.18 SSV.dial :exhaust @21:45:54.04 Exhaust.select :open @21:45:54.17 SS.reconfigure SSexhale @21:45:54.23 SS.seek 0ml @21:46:04.04 Exhaust.select :close @21:46:04.15 SSV.dial CBV @21:46:04.18 CBV.dial SSV @21:46:05.07 Allowing 130s for 25.0ml of remaining 550.0ml @21:46:05.08 Intake.select :open @21:46:07.28 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:46:07.29 SS.reconfigure SSinhale.with maxSpeed: 19 @21:46:07.37 SS.seek 25ml @21:47:41.25 Stroke completed -- waiting for pressure to equalize ... @21:47:54.07 Intake.select :close @21:47:54.23 SSV.dial :exhaust @21:47:55.12 Exhaust.select :open @21:47:55.25 SS.reconfigure SSexhale @21:47:55.31 SS.seek 0ml @21:48:04.93 Exhaust.select :close @21:48:05.04 SSV.dial CBV @21:48:05.07 CBV.dial SSV @21:48:05.96 Allowing 130s for 25.0ml of remaining 525.0ml @21:48:05.98 Intake.select :open @21:48:08.17 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:48:08.18 SS.reconfigure SSinhale.with maxSpeed: 19 @21:48:08.26 SS.seek 25ml @21:49:42.07 Stroke completed -- waiting for pressure to equalize ... @21:49:56.17 Intake.select :close @21:49:56.33 SSV.dial :exhaust @21:49:57.19 Exhaust.select :open @21:49:57.32 SS.reconfigure SSexhale @21:49:57.38 SS.seek 0ml @21:50:06.96 Exhaust.select :close @21:50:07.08 SSV.dial CBV @21:50:07.11 CBV.dial SSV @21:50:07.99 Allowing 130s for 25.0ml of remaining 500.0ml @21:50:08.00 Intake.select :open @21:50:10.20 Intake @15.8psia, vacuum from 9.3 to 13.3psi @21:50:10.21 SS.reconfigure SSinhale.with maxSpeed: 19 @21:50:10.29 SS.seek 25ml @21:51:43.99 Stroke completed -- waiting for pressure to equalize ... @21:51:58.10 Intake.select :close @21:51:58.26 SSV.dial :exhaust @21:51:59.15 Exhaust.select :open @21:51:59.28 SS.reconfigure SSexhale @21:51:59.34 SS.seek 0ml @21:52:08.94 Exhaust.select :close @21:52:09.05 SSV.dial CBV @21:52:09.08 CBV.dial SSV @21:52:09.97 Allowing 130s for 25.0ml of remaining 475.0ml @21:52:09.98 Intake.select :open @21:52:12.17 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:52:12.20 SS.reconfigure SSinhale.with maxSpeed: 19 @21:52:12.27 SS.seek 25ml @21:53:46.06 Stroke completed -- waiting for pressure to equalize ... @21:54:00.08 Intake.select :close @21:54:00.24 SSV.dial :exhaust @21:54:01.11 Exhaust.select :open @21:54:01.24 SS.reconfigure SSexhale @21:54:01.30 SS.seek 0ml @21:54:11.18 Exhaust.select :close @21:54:11.29 SSV.dial CBV @21:54:11.32 CBV.dial SSV @21:54:12.19 Allowing 130s for 25.0ml of remaining 450.0ml @21:54:12.21 Intake.select :open @21:54:14.40 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:54:14.41 SS.reconfigure SSinhale.with maxSpeed: 19 @21:54:14.49 SS.seek 25ml @21:55:48.06 Stroke completed -- waiting for pressure to equalize ... @21:56:00.95 Intake.select :close @21:56:01.11 SSV.dial :exhaust @21:56:01.99 Exhaust.select :open @21:56:02.13 SS.reconfigure SSexhale @21:56:02.19 SS.seek 0ml @21:56:11.99 Exhaust.select :close @21:56:12.11 SSV.dial CBV @21:56:12.14 CBV.dial SSV @21:56:13.03 Allowing 130s for 25.0ml of remaining 425.0ml @21:56:13.04 Intake.select :open @21:56:15.24 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:56:15.26 SS.reconfigure SSinhale.with maxSpeed: 19 @21:56:15.33 SS.seek 25ml @21:57:49.24 Stroke completed -- waiting for pressure to equalize ... @21:58:02.05 Intake.select :close @21:58:02.20 SSV.dial :exhaust @21:58:03.08 Exhaust.select :open @21:58:03.21 SS.reconfigure SSexhale @21:58:03.27 SS.seek 0ml @21:58:12.83 Exhaust.select :close @21:58:12.94 SSV.dial CBV @21:58:12.97 CBV.dial SSV @21:58:13.86 Allowing 130s for 25.0ml of remaining 400.0ml @21:58:13.87 Intake.select :open @21:58:16.07 Intake @15.7psia, vacuum from 9.2 to 13.2psi @21:58:16.08 SS.reconfigure SSinhale.with maxSpeed: 19 @21:58:16.16 SS.seek 25ml @21:59:49.90 Stroke completed -- waiting for pressure to equalize ... @22:00:04.69 Intake.select :close @22:00:04.85 SSV.dial :exhaust @22:00:05.72 Exhaust.select :open @22:00:05.85 SS.reconfigure SSexhale @22:00:05.92 SS.seek 0ml @22:00:15.58 Exhaust.select :close @22:00:15.70 SSV.dial CBV @22:00:15.73 CBV.dial SSV @22:00:16.61 Allowing 130s for 25.0ml of remaining 375.0ml @22:00:16.63 Intake.select :open @22:00:18.81 Intake @15.7psia, vacuum from 9.2 to 13.2psi @22:00:18.82 SS.reconfigure SSinhale.with maxSpeed: 19 @22:00:18.91 SS.seek 25ml @22:01:52.65 Stroke completed -- waiting for pressure to equalize ... @22:02:06.76 Intake.select :close @22:02:06.92 SSV.dial :exhaust @22:02:07.80 Exhaust.select :open @22:02:07.93 SS.reconfigure SSexhale @22:02:07.99 SS.seek 0ml @22:02:17.87 Exhaust.select :close @22:02:18.00 SSV.dial CBV @22:02:18.03 CBV.dial SSV @22:02:18.92 Allowing 130s for 25.0ml of remaining 350.0ml @22:02:18.93 Intake.select :open @22:02:21.13 Intake @15.7psia, vacuum from 9.2 to 13.2psi @22:02:21.14 SS.reconfigure SSinhale.with maxSpeed: 19 @22:02:21.22 SS.seek 25ml @22:03:54.83 Stroke completed -- waiting for pressure to equalize ... @22:04:07.00 Intake.select :close @22:04:07.16 SSV.dial :exhaust @22:04:08.03 Exhaust.select :open @22:04:08.16 SS.reconfigure SSexhale @22:04:08.22 SS.seek 0ml @22:04:17.92 Exhaust.select :close @22:04:18.03 SSV.dial CBV @22:04:18.06 CBV.dial SSV @22:04:18.94 Allowing 130s for 25.0ml of remaining 325.0ml @22:04:18.95 Intake.select :open @22:04:21.14 Intake @15.7psia, vacuum from 9.2 to 13.2psi @22:04:21.15 SS.reconfigure SSinhale.with maxSpeed: 19 @22:04:21.23 SS.seek 25ml @22:05:55.01 Stroke completed -- waiting for pressure to equalize ... @22:06:09.11 Intake.select :close @22:06:09.27 SSV.dial :exhaust @22:06:10.16 Exhaust.select :open @22:06:10.29 SS.reconfigure SSexhale @22:06:10.35 SS.seek 0ml @22:06:19.93 Exhaust.select :close @22:06:20.07 SSV.dial CBV @22:06:20.09 CBV.dial SSV @22:06:20.98 Allowing 130s for 25.0ml of remaining 300.0ml @22:06:21.00 Intake.select :open @22:06:23.19 Intake @15.7psia, vacuum from 9.2 to 13.2psi @22:06:23.21 SS.reconfigure SSinhale.with maxSpeed: 19 @22:06:23.29 SS.seek 25ml @22:07:57.26 Stroke completed -- waiting for pressure to equalize ... @22:08:11.98 Intake.select :close @22:08:12.14 SSV.dial :exhaust @22:08:13.00 Exhaust.select :open @22:08:13.13 SS.reconfigure SSexhale @22:08:13.19 SS.seek 0ml @22:08:22.73 Exhaust.select :close @22:08:22.84 SSV.dial CBV @22:08:22.87 CBV.dial SSV @22:08:23.73 Allowing 130s for 25.0ml of remaining 275.0ml @22:08:23.75 Intake.select :open @22:08:25.93 Intake @15.7psia, vacuum from 9.2 to 13.2psi @22:08:25.96 SS.reconfigure SSinhale.with maxSpeed: 19 @22:08:26.03 SS.seek 25ml @22:10:00.24 Stroke completed -- waiting for pressure to equalize ... @22:10:14.88 Intake.select :close @22:10:15.04 SSV.dial :exhaust @22:10:15.92 Exhaust.select :open @22:10:16.05 SS.reconfigure SSexhale @22:10:16.11 SS.seek 0ml @22:10:25.94 Exhaust.select :close @22:10:26.06 SSV.dial CBV @22:10:26.09 CBV.dial SSV @22:10:26.98 Allowing 130s for 25.0ml of remaining 250.0ml @22:10:27.00 Intake.select :open @22:10:29.19 Intake @15.7psia, vacuum from 9.2 to 13.2psi @22:10:29.20 SS.reconfigure SSinhale.with maxSpeed: 19 @22:10:29.28 SS.seek 25ml @22:12:03.11 Stroke completed -- waiting for pressure to equalize ... @22:12:16.65 Intake.select :close @22:12:16.81 SSV.dial :exhaust @22:12:17.67 Exhaust.select :open @22:12:17.80 SS.reconfigure SSexhale @22:12:17.86 SS.seek 0ml @22:12:27.46 Exhaust.select :close @22:12:28.12 SSV.dial CBV @22:12:28.15 CBV.dial SSV @22:12:29.03 Allowing 130s for 25.0ml of remaining 225.0ml @22:12:29.05 Intake.select :open @22:12:31.24 Intake @15.7psia, vacuum from 9.2 to 13.2psi @22:12:31.25 SS.reconfigure SSinhale.with maxSpeed: 19 @22:12:31.33 SS.seek 25ml @22:14:05.27 Stroke completed -- waiting for pressure to equalize ... @22:14:19.38 Intake.select :close @22:14:19.53 SSV.dial :exhaust @22:14:20.42 Exhaust.select :open @22:14:20.55 SS.reconfigure SSexhale @22:14:20.61 SS.seek 0ml @22:14:30.40 Exhaust.select :close @22:14:30.52 SSV.dial CBV @22:14:30.55 CBV.dial SSV @22:14:31.44 Allowing 130s for 25.0ml of remaining 200.0ml @22:14:31.45 Intake.select :open @22:14:33.65 Intake @15.8psia, vacuum from 9.3 to 13.3psi @22:14:33.66 SS.reconfigure SSinhale.with maxSpeed: 19 @22:14:33.74 SS.seek 25ml @22:16:07.58 Stroke completed -- waiting for pressure to equalize ... @22:16:20.99 Intake.select :close @22:16:21.15 SSV.dial :exhaust @22:16:22.04 Exhaust.select :open @22:16:22.17 SS.reconfigure SSexhale @22:16:22.23 SS.seek 0ml @22:16:31.83 Exhaust.select :close @22:16:31.94 SSV.dial CBV @22:16:31.97 CBV.dial SSV @22:16:32.86 Allowing 130s for 25.0ml of remaining 175.0ml @22:16:32.87 Intake.select :open @22:16:35.06 Intake @15.7psia, vacuum from 9.2 to 13.2psi @22:16:35.07 SS.reconfigure SSinhale.with maxSpeed: 19 @22:16:35.15 SS.seek 25ml @22:18:09.03 Stroke completed -- waiting for pressure to equalize ... @22:18:23.76 Intake.select :close @22:18:23.92 SSV.dial :exhaust @22:18:24.78 Exhaust.select :open @22:18:24.91 SS.reconfigure SSexhale @22:18:24.97 SS.seek 0ml @22:18:34.55 Exhaust.select :close @22:18:34.67 SSV.dial CBV @22:18:34.70 CBV.dial SSV @22:18:35.59 Allowing 130s for 25.0ml of remaining 150.0ml @22:18:35.60 Intake.select :open @22:18:38.26 Intake @15.8psia, vacuum from 9.3 to 13.3psi @22:18:38.28 SS.reconfigure SSinhale.with maxSpeed: 19 @22:18:38.37 SS.seek 25ml @22:20:12.20 Stroke completed -- waiting for pressure to equalize ... @22:20:26.93 Intake.select :close @22:20:27.09 SSV.dial :exhaust @22:20:27.98 Exhaust.select :open @22:20:28.11 SS.reconfigure SSexhale @22:20:28.18 SS.seek 0ml @22:20:38.16 Exhaust.select :close @22:20:38.29 SSV.dial CBV @22:20:38.32 CBV.dial SSV @22:20:39.21 Allowing 130s for 25.0ml of remaining 125.0ml @22:20:39.22 Intake.select :open @22:20:41.42 Intake @15.7psia, vacuum from 9.2 to 13.2psi @22:20:41.43 SS.reconfigure SSinhale.with maxSpeed: 19 @22:20:41.51 SS.seek 25ml @22:22:15.36 Stroke completed -- waiting for pressure to equalize ... @22:22:28.16 Intake.select :close @22:22:28.32 SSV.dial :exhaust @22:22:29.20 Exhaust.select :open @22:22:29.33 SS.reconfigure SSexhale @22:22:29.39 SS.seek 0ml @22:22:39.18 Exhaust.select :close @22:22:39.29 SSV.dial CBV @22:22:39.32 CBV.dial SSV @22:22:40.20 Allowing 130s for 25.0ml of remaining 100.0ml @22:22:40.22 Intake.select :open @22:22:42.41 Intake @15.8psia, vacuum from 9.3 to 13.3psi @22:22:42.42 SS.reconfigure SSinhale.with maxSpeed: 19 @22:22:42.50 SS.seek 25ml @22:24:16.25 Stroke completed -- waiting for pressure to equalize ... @22:24:29.75 Intake.select :close @22:24:29.91 SSV.dial :exhaust @22:24:30.79 Exhaust.select :open @22:24:30.92 SS.reconfigure SSexhale @22:24:30.98 SS.seek 0ml @22:24:40.64 Exhaust.select :close @22:24:40.76 SSV.dial CBV @22:24:40.79 CBV.dial SSV @22:24:41.68 Allowing 130s for 25.0ml of remaining 74.9ml @22:24:41.69 Intake.select :open @22:24:43.88 Intake @15.8psia, vacuum from 9.3 to 13.3psi @22:24:43.91 SS.reconfigure SSinhale.with maxSpeed: 19 @22:24:43.98 SS.seek 25ml @22:26:17.86 Stroke completed -- waiting for pressure to equalize ... @22:26:31.98 Intake.select :close @22:26:32.14 SSV.dial :exhaust @22:26:33.03 Exhaust.select :open @22:26:33.16 SS.reconfigure SSexhale @22:26:33.22 SS.seek 0ml @22:26:43.00 Exhaust.select :close @22:26:43.12 SSV.dial CBV @22:26:43.15 CBV.dial SSV @22:26:44.03 Allowing 130s for 25.0ml of remaining 49.9ml @22:26:44.04 Intake.select :open @22:26:46.29 Intake @15.7psia, vacuum from 9.2 to 13.2psi @22:26:46.32 SS.reconfigure SSinhale.with maxSpeed: 19 @22:26:46.39 SS.seek 25ml @22:28:19.94 Stroke completed -- waiting for pressure to equalize ... @22:28:34.12 Intake.select :close @22:28:34.27 SSV.dial :exhaust @22:28:35.15 Exhaust.select :open @22:28:35.28 SS.reconfigure SSexhale @22:28:35.34 SS.seek 0ml @22:28:45.15 Exhaust.select :close @22:28:45.26 SSV.dial CBV @22:28:45.29 CBV.dial SSV @22:28:46.18 Allowing 130s for 24.9ml of remaining 24.9ml @22:28:46.19 Intake.select :open @22:28:48.39 Intake @15.8psia, vacuum from 9.3 to 13.3psi @22:28:48.40 SS.reconfigure SSinhale.with maxSpeed: 19 @22:28:48.48 SS.seek 24.95ml @22:30:22.34 Stroke completed -- waiting for pressure to equalize ... @22:30:37.06 Intake.select :close @22:30:37.22 SSV.dial :exhaust @22:30:38.10 Exhaust.select :open @22:30:38.23 SS.reconfigure SSexhale @22:30:38.30 SS.seek 0ml @22:30:48.01 Exhaust.select :close @22:30:48.15 Sampled 1500.0ml @22:30:48.17 Drying puck before fixing for 4:00 @22:30:48.19 CBV.dial :waste2b @22:30:49.08 delay 1:00 @22:30:51.16 Gate.power :sampler,:OFF @22:31:49.10 CSV.dial :air @22:31:49.63 CS.reconfigure CSfast @22:31:49.70 CS.seek 10ml @22:32:17.09 Pumping wcr puck from tube 2 up to 55psi... @22:32:17.10 CBV.dial SSV @22:32:18.56 CBV.dial between CSV and waste2b @22:32:19.68 CSV.dial between air and CTV @22:32:20.08 CS.seek 7.115ml @22:32:28.68 CTV.dial CSV,avoiding: :air @22:32:28.71 CSV.dial CTV @22:32:29.60 CS.reconfigure CSpressurize @22:32:29.67 CS.seek 0ml @22:32:48.68 CSV.dial :air @22:32:49.23 CS.reconfigure CSfast @22:32:49.30 CS.seek 10ml @22:33:15.95 CSV.dial between air and CTV @22:33:16.33 CS.seek 2.004ml @22:33:38.05 CTV.dial CSV,avoiding: :air @22:33:38.08 CSV.dial CTV @22:33:38.46 CS.reconfigure CSpressurize @22:33:38.53 CS.seek 0ml @22:33:39.47 CBV.dial :waste2b @22:33:39.87 CS.reconfigure CSconfig @22:33:39.94 CS.seek :empty @22:33:45.80 delay 1:00 @22:34:45.82 CTV.dial :air,avoiding: :intake @22:34:47.35 Fix #1 of 2 (with rnal) for 2:00 @22:34:47.37 CSV.dial CSR @22:34:47.95 Pulling 2ml of CSR.rnal @22:34:47.96 CSR.select :rnal @22:34:48.10 CS.seek 2ml @22:34:54.24 delay 5 seconds @22:34:59.26 CSR.select :air @22:34:59.40 CS.reconfigure CSfast @22:34:59.46 CS.seek 10ml @22:35:20.93 Pumping wcr puck from tube 2 up to 55psi... @22:35:20.94 CBV.dial SSV @22:35:22.40 CBV.dial between CSV and waste2b @22:35:23.54 CSV.dial between air and CTV @22:35:24.25 CS.seek 7.127ml @22:35:32.68 CTV.dial CSV,avoiding: :air @22:35:32.71 CSV.dial CTV @22:35:34.31 CS.reconfigure CSpressurize @22:35:34.38 CS.seek 0ml @22:35:53.96 CSV.dial :air @22:35:54.51 CS.reconfigure CSfast @22:35:54.58 CS.seek 10ml @22:36:21.12 CSV.dial between air and CTV @22:36:21.50 CS.seek 2.001ml @22:36:43.03 CTV.dial CSV,avoiding: :air @22:36:43.06 CSV.dial CTV @22:36:43.43 CS.reconfigure CSpressurize @22:36:43.50 CS.seek 0ml @22:36:44.42 delay 2:00 @22:38:44.44 CBV.dial :waste2b @22:38:44.82 CS.reconfigure CSconfig @22:38:44.89 CS.seek :empty @22:38:50.68 delay 1:00 @22:39:50.70 CTV.dial :air,avoiding: :intake @22:39:52.25 Fix #2 of 2 (with rnal) for 2:00 @22:39:52.27 CSV.dial CSR @22:39:52.86 Pulling 2ml of CSR.rnal @22:39:52.87 CSR.select :rnal @22:39:53.02 CS.seek 2ml @22:39:59.15 delay 5 seconds @22:40:04.17 CSR.select :air @22:40:04.31 CS.reconfigure CSfast @22:40:04.37 CS.seek 10ml @22:40:25.68 Pumping wcr puck from tube 2 up to 55psi... @22:40:25.69 CBV.dial SSV @22:40:27.15 CBV.dial between CSV and waste2b @22:40:28.26 CSV.dial between air and CTV @22:40:28.99 CS.seek 7.165ml @22:40:37.31 CTV.dial CSV,avoiding: :air @22:40:37.34 CSV.dial CTV @22:40:38.90 CS.reconfigure CSpressurize @22:40:38.97 CS.seek 0ml @22:40:58.03 CSV.dial :air @22:40:58.70 CS.reconfigure CSfast @22:40:58.77 CS.seek 10ml @22:41:25.39 CSV.dial between air and CTV @22:41:26.27 CS.seek 2.023ml @22:41:48.00 CTV.dial CSV,avoiding: :air @22:41:48.03 CSV.dial CTV @22:41:48.40 CS.reconfigure CSpressurize @22:41:48.47 CS.seek 0ml @22:41:49.49 delay 2:00 @22:43:49.51 CBV.dial :waste2b @22:43:49.87 CS.reconfigure CSconfig @22:43:49.94 CS.seek :empty @22:43:55.78 delay 1:00 @22:44:55.80 CTV.dial :air,avoiding: :intake @22:44:57.42 Storing wcr puck in Collection Clamp to tube 1 @22:44:57.61 SC.reconfigure SCtight @22:44:57.67 SC.seek 1 @22:44:59.86 SC.reconfigure SCloose @22:45:00.08 Elbow.reconfigure TightElbow @22:45:00.14 Elbow.seek 1 @22:45:02.19 Forearm.reconfigure TightForearm @22:45:02.25 Forearm.seek 1 + 700 counts @22:45:03.29 SE.reconfigure SEpick @22:45:03.38 SE.seek beamBreak @22:45:06.53 SE.configure SEconfig @22:45:06.69 Elbow.reconfigure LimpElbow @22:45:06.76 Forearm.reconfigure LooseForearm @22:45:06.82 Forearm.seek 1 @22:45:07.16 Elbow.hold @22:45:07.24 Elbow.reconfigure TightElbow @22:45:07.35 SE.seek 1.07 Pucks below Top Plate @22:45:07.80 Forearm.force -20 @22:45:07.84 Forearm.seek 1 + 325 counts @22:45:08.19 Forearm.reconfigure TightForearm @22:45:08.25 Forearm.seek 1 + 700 counts @22:45:08.48 SE.seek 2.07 Pucks below Top Plate @22:45:09.24 Forearm.reconfigure LooseForearm @22:45:09.30 Forearm.seek :retract @22:45:10.30 Elbow.seek CC @22:45:11.97 Elbow.reconfigure LooseElbow @22:45:12.08 Forearm.seek CC @22:45:13.16 Hand.close @22:45:14.38 CC.seek :open @22:45:18.95 Forearm.seek :retract @22:45:20.04 Elbow.reconfigure TightElbow @22:45:20.11 Elbow.seek 1 @22:45:22.13 Elbow.reconfigure LimpElbow @22:45:22.20 Forearm.seek 1 @22:45:23.26 Elbow.hold @22:45:23.35 Elbow.reconfigure TightElbow @22:45:23.41 Hand.open @22:45:24.27 Forearm.force -20 @22:45:24.33 Forearm.seek 1 + 350 counts @22:45:24.66 Forearm.reconfigure TightForearm @22:45:24.72 Forearm.seek 1 + 700 counts @22:45:25.02 SE.seek :down @22:45:26.14 Forearm.reconfigure LooseForearm @22:45:26.21 Forearm.seek :retract @22:45:27.80 SE.coast @22:45:27.85 SC.coast @22:45:27.91 Starting finalPurge @22:45:27.92 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:45:28.02 Hand.open @22:45:28.41 Elbow.seek FlushPuck::Garage @22:45:30.40 Elbow.reconfigure LooseElbow @22:45:30.51 Forearm.seek :garage @22:45:31.54 Hand.close @22:45:32.41 Forearm.seek :retract @22:45:33.48 Elbow.reconfigure TightElbow @22:45:33.54 Elbow.seek CC @22:45:36.46 Elbow.reconfigure LooseElbow @22:45:36.57 Forearm.reconfigure TightForearm @22:45:36.64 Forearm.seek CC @22:45:37.98 CC.seek :closed @22:45:42.33 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.33 @22:45:42.42 Hand.open @22:45:43.22 Forearm.reconfigure LooseForearm @22:45:43.28 Forearm.seek :retract @22:45:44.32 Flushing the Collection Valve::Manifold @22:45:44.98 CSV.dial CSR @22:45:45.53 Pulling 5ml of CSR.flush @22:45:45.54 CSR.select :flush @22:45:45.69 CS.seek 5ml @22:45:59.47 delay 5 seconds @22:46:04.49 CSR.select :air @22:46:04.67 CS.reconfigure CSfast @22:46:04.73 CS.seek 7.5ml @22:46:12.19 CSV.dial :waste @22:46:13.70 CS.reconfigure CSconfig @22:46:13.77 CS.seek 0ml @22:46:33.87 Flushing lower manifold's waste1 port @22:46:34.01 CSV.dial :flush @22:46:34.53 CS.reconfigure CSfast @22:46:34.60 CS.seek 5ml @22:46:48.34 delay 5 seconds @22:46:53.36 CSV.dial :air @22:46:53.94 CS.seek 7ml @22:47:00.06 CSV.dial CTV @22:47:00.09
CTV.dial CSV @22:47:01.70 CBV.dial :waste1 @22:47:02.59 CS.seek 0ml @22:47:21.47 Flushing lower manifold's waste2b port @22:47:21.61 CSV.dial :flush @22:47:22.49 CS.seek 5ml @22:47:36.31 delay 5 seconds @22:47:41.33 CSV.dial :air @22:47:41.94 CS.seek 7ml @22:47:47.94 CSV.dial CTV @22:47:47.97
CTV.dial CSV @22:47:48.47 CBV.dial :waste2b @22:47:49.34 CS.seek 0ml @22:48:08.06 Flushing lower manifold's waste1 port @22:48:08.07 CSV.dial CSR @22:48:08.66 Pulling 4ml of CSR.mfbkill @22:48:08.67 CSR.select :mfbkill @22:48:08.82 CS.reconfigure CSconfig @22:48:08.88 CS.seek 4ml @22:48:20.28 delay 5 seconds @22:48:25.30 CSR.select :air @22:48:25.45 CS.reconfigure CSfast @22:48:25.51 CS.seek 10ml @22:48:41.84 CSV.dial CTV @22:48:41.87
CTV.dial CSV @22:48:42.41 CBV.dial :waste1 @22:48:43.31 CS.seek 0ml @22:49:09.81 Flushing lower manifold's waste2b port @22:49:09.82 CSV.dial CSR @22:49:10.43 Pulling 4ml of CSR.mfbkill @22:49:10.44 CSR.select :mfbkill @22:49:10.59 CS.reconfigure CSconfig @22:49:10.65 CS.seek 4ml @22:49:21.94 delay 5 seconds @22:49:26.96 CSR.select :air @22:49:27.10 CS.reconfigure CSfast @22:49:27.16 CS.seek 10ml @22:49:43.44 CSV.dial CTV @22:49:43.47
CTV.dial CSV @22:49:44.02 CBV.dial :waste2b @22:49:45.42 CS.seek 0ml @22:50:11.66 Killing upper manifold @22:50:11.67 CSV.dial CSR @22:50:12.39 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @22:50:12.54 CS.reconfigure CSconfig @22:50:12.60 CS.seek 4ml @22:50:23.91 delay 5 seconds @22:50:28.93 CSR.select :air @22:50:29.07 CS.reconfigure CSfast @22:50:29.13 CS.seek 10ml @22:50:45.28 CSV.dial CBV @22:50:45.31
CBV.dial CSV @22:50:46.55 CTV.dial :air @22:50:48.06 CS.seek 0ml @22:51:14.38 delay 7 seconds @22:51:21.41 CS.seek 3ml @22:51:30.03 CSV.dial :waste @22:51:30.55 CS.seek 0ml @22:51:39.19 Flushing upper manifold @22:51:39.32 CSV.dial :flush @22:51:39.85 CS.seek 5ml @22:51:53.44 delay 5 seconds @22:51:58.46 CSV.dial :air @22:51:59.05 CS.seek 7ml @22:52:05.19 CSV.dial CBV @22:52:05.22
CBV.dial CSV @22:52:06.42 CTV.dial :air @22:52:06.44 CS.seek 0ml @22:52:25.07 delay 7 seconds @22:52:32.10 CS.seek 3ml @22:52:40.78 CSV.dial :waste @22:52:41.32 CS.seek 0ml @22:52:49.96 CSV.dial :flush @22:52:50.47 CS.seek 3ml @22:52:59.07 delay 5 seconds @22:53:04.09 CSV.dial :air @22:53:04.66 CS.seek 5ml @22:53:10.69 CSV.dial :air @22:53:10.71 CS.seek 0ml @22:53:24.32 delay 7 seconds @22:53:26.89 PCR.off @22:53:29.59 PCR for IPc completed -- closed /var/log/esp/IPc19may1621h1500ml.pcr @22:53:29.66 Cleaning PCR @22:53:29.68 AFV.select :carrierSolution @22:53:29.80 delay 2 seconds @22:53:31.35
CS.seek 3ml @22:53:31.82 AS.reconfigure ASconfig @22:53:31.89 AS.seek 0.5ml @22:53:39.94
CSV.dial :waste @22:53:40.81 CS.seek 0ml @22:53:42.20 delay 1 second @22:53:43.22 AFV.select :holdingCoil @22:53:43.32 ASV.dial :waste1 @22:53:43.73 ATV.dial :air3 @22:53:45.07 AS.reconfigure ASslow1 @22:53:45.13 AS.seek 0.51ml @22:53:47.43 delay 2 seconds @22:53:49.44
Flushing lower manifold's waste1 port @22:53:49.56 ATV.dial :bleach @22:53:49.63
CSV.dial :flush @22:53:50.22 CS.seek 5ml @22:53:50.25 AS.seek 0.81ml @22:54:03.88
delay 5 seconds @22:54:08.90 CSV.dial :air @22:54:09.45 CS.seek 7ml @22:54:15.47 CSV.dial CTV @22:54:15.50
CTV.dial CSV @22:54:17.08 CBV.dial :waste1 @22:54:17.61 CS.seek 0ml @22:54:36.29 Flushing lower manifold's waste2b port @22:54:36.43 CSV.dial :flush @22:54:37.33 CS.seek 5ml @22:54:50.89 delay 3 seconds @22:54:50.94
delay 5 seconds @22:54:53.91 ATV.dial :air3 @22:54:54.47 delay 3 seconds @22:54:55.96
CSV.dial :air @22:54:56.56 CS.seek 7ml @22:54:57.54 AS.seek 0.835ml @22:55:02.57 CSV.dial CTV @22:55:02.60
CTV.dial CSV @22:55:02.84 delay 3 seconds @22:55:03.13
CBV.dial :waste2b @22:55:04.00 CS.seek 0ml @22:55:05.86 ATV.dial :bleach @22:55:06.49 AS.seek 0.82ml @22:55:09.82 delay 3 seconds @22:55:12.84 ARV.dial :waste @22:55:13.47 delay 3 seconds @22:55:16.49 ATV.dial :ARV @22:55:17.40 AS.reconfigure ASconfig @22:55:17.46 AS.seek 0.47ml @22:55:22.69
Garaging FlushPuck in Collection Clamp @22:55:22.78 Elbow.reconfigure TightElbow @22:55:22.84 Elbow.seek CC @22:55:23.10 Elbow.reconfigure LooseElbow @22:55:23.21 Forearm.seek CC @22:55:24.30 Hand.close @22:55:25.05 CC.seek :open @22:55:29.46 Forearm.seek :retract @22:55:30.58 Elbow.reconfigure TightElbow @22:55:30.64 Elbow.seek :garage @22:55:34.42 Elbow.reconfigure LooseElbow @22:55:34.53 Forearm.reconfigure TightForearm @22:55:34.59 Forearm.seek :garage @22:55:35.77 Hand.open @22:55:36.61 Forearm.reconfigure LooseForearm @22:55:36.67 Forearm.seek :retract @22:55:37.74 Starting initialPurge @22:55:37.75 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:55:37.84 Hand.open @22:55:37.91 AFV.select :carrierSolution @22:55:38.08 delay 2 seconds @22:55:38.13
Elbow.reconfigure TightElbow @22:55:38.19 Elbow.seek FlushPuck::Garage @22:55:38.32 Elbow.reconfigure LooseElbow @22:55:38.43 Forearm.seek :garage @22:55:39.48 Hand.close @22:55:40.12 AS.seek 1ml @22:55:40.41
Forearm.seek :retract @22:55:41.47 Elbow.reconfigure TightElbow @22:55:41.53 Elbow.seek CC @22:55:44.45 Elbow.reconfigure LooseElbow @22:55:44.57 Forearm.reconfigure TightForearm @22:55:44.63 Forearm.seek CC @22:55:45.97 CC.seek :closed @22:55:50.19 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.32 @22:55:50.29 Hand.open @22:55:51.11 Forearm.reconfigure LooseForearm @22:55:51.17 Forearm.seek :retract @22:55:52.21 Flushing the Collection Valve::Manifold @22:55:52.34 CSV.dial CSR @22:55:52.90 Pulling 5ml of CSR.flush @22:55:52.91 CSR.select :flush @22:55:53.06 CS.reconfigure CSconfig @22:55:53.12 CS.seek 5ml @22:56:06.97 delay 5 seconds @22:56:10.87 delay 1 second @22:56:11.89 AFV.select :holdingCoil @22:56:11.99
CSR.select :air @22:56:12.06 delay 1 second @22:56:12.17
CS.reconfigure CSfast @22:56:12.23 CS.seek 7.5ml @22:56:13.08 AS.reconfigure ASfast @22:56:13.15 AS.seek 0ml @22:56:19.54
CSV.dial :waste @22:56:21.05 CS.reconfigure CSconfig @22:56:21.12 CS.seek 0ml @22:56:40.47 AFV.select :carrierSolution @22:56:40.60 delay 2 seconds @22:56:41.51
Purging external Intake @22:56:41.64 CSV.dial :flush @22:56:42.13 CS.reconfigure CSfast @22:56:42.19 CS.seek 10ml @22:56:42.62 AS.reconfigure ASconfig @22:56:42.69 AS.seek 1ml @22:57:08.47
delay 5 seconds @22:57:13.49 CSV.dial CBV @22:57:13.53
CBV.dial CSV @22:57:14.44 CTV.dial :intake @22:57:15.30 Intake.select :open @22:57:15.43 CS.seek 0ml @22:57:40.45 delay 1 second @22:57:41.47 AFV.select :holdingCoil @22:57:41.57 delay 1 second @22:57:42.59 AS.reconfigure ASfast @22:57:42.65 AS.seek 0ml @22:57:43.00
Intake.select :close @22:57:43.12 CTV.dial :air @22:57:43.97 Flushing upper manifold @22:57:44.10 CSV.dial :flush @22:57:45.48 CS.seek 5ml @22:57:59.26 delay 5 seconds @22:58:04.28 CSV.dial :air @22:58:04.85 CS.seek 7ml @22:58:10.01 AFV.select :carrierSolution @22:58:10.13 delay 2 seconds @22:58:10.94 CSV.dial CBV @22:58:10.97
CBV.dial CSV @22:58:12.16 CTV.dial :air @22:58:12.18 CS.seek 0ml @22:58:12.25 AS.reconfigure ASconfig @22:58:12.32 AS.seek 1ml @22:58:30.79
delay 7 seconds @22:58:37.82 CS.seek 3ml @22:58:46.41 CSV.dial :waste @22:58:46.93 CS.seek 0ml @22:58:55.64 CSV.dial :flush @22:58:56.17 CS.seek 3ml @22:59:04.73 delay 5 seconds @22:59:09.75 CSV.dial :air @22:59:10.06 delay 1 second @22:59:10.32
CS.seek 5ml @22:59:11.08 AFV.select :holdingCoil @22:59:11.19 delay 1 second @22:59:12.21 AS.reconfigure ASfast @22:59:12.27 AS.seek 0ml @22:59:16.32
CSV.dial :air @22:59:16.34 CS.seek 0ml @22:59:29.94 delay 7 seconds @22:59:36.98 CS.seek 3ml @22:59:39.62 ARV.dial between carrier and air1 @22:59:40.14 ATV.dial :air3 @22:59:41.48 AS.reconfigure ASslow1 @22:59:41.54 AS.seek 0.01ml @22:59:43.84 delay 2 seconds @22:59:45.79
CSV.dial :waste @22:59:45.85 ATV.dial :bleach @22:59:46.49 AS.seek 0.81ml @22:59:46.65
CS.seek 0ml @22:59:55.13 Garaging FlushPuck in Collection Clamp @22:59:55.22 Elbow.reconfigure TightElbow @22:59:55.28 Elbow.seek CC @22:59:55.51 Elbow.reconfigure LooseElbow @22:59:55.62 Forearm.seek CC @22:59:56.68 Hand.close @22:59:57.43 CC.seek :open @23:00:01.84 Forearm.seek :retract @23:00:02.88 Elbow.reconfigure TightElbow @23:00:02.94 Elbow.seek :garage @23:00:06.12 Elbow.reconfigure LooseElbow @23:00:06.23 Forearm.reconfigure TightForearm @23:00:06.29 Forearm.seek :garage @23:00:07.46 Hand.open @23:00:08.24 Forearm.reconfigure LooseForearm @23:00:08.30 Forearm.seek :retract @23:00:09.36 Completed [mbaPhase] begun 18:18:54 @23:00:09.38 Extending 190516totalsampletest until completion of MFBPCR @23:02:27.64 delay 3 seconds @23:02:30.66 ATV.dial :air3 @23:02:31.23 delay 3 seconds @23:02:34.30 AS.seek 0.835ml @23:02:39.69 delay 3 seconds @23:02:42.71 ATV.dial :bleach @23:02:43.35 AS.seek 0.82ml @23:02:46.68 delay 3 seconds @23:02:49.70 ATV.dial PCR @23:02:50.12 AS.reconfigure ASconfig @23:02:50.18 AS.seek :empty @23:03:37.65 PCR.program :bleachProfile @23:03:37.70 PCR program should complete in < 2:45 @23:03:37.71 PCR.run 10:00 @23:06:33.99 PCR.off @23:06:34.02 AFV.select :carrierSolution @23:06:34.14 delay 2 seconds @23:06:36.17 AS.seek 1ml @23:07:34.03 delay 1 second @23:07:35.05 AFV.select :holdingCoil @23:07:35.15 delay 1 second @23:07:36.17 AS.reconfigure ASfast @23:07:36.24 AS.seek 0ml @23:08:03.57 delay 3 seconds @23:08:06.59 AFV.select :carrierSolution @23:08:06.69 delay 2 seconds @23:08:08.72 AS.reconfigure ASconfig @23:08:08.79 AS.seek 1ml @23:09:06.56 delay 1 second @23:09:07.58 AFV.select :holdingCoil @23:09:07.68 delay 1 second @23:09:08.70 AS.reconfigure ASfast @23:09:08.76 AS.seek 0ml @23:09:36.12 delay 3 seconds @23:09:39.14 AFV.select :carrierSolution @23:09:39.24 delay 2 seconds @23:09:41.26 AS.reconfigure ASconfig @23:09:41.33 AS.seek 1ml @23:10:39.14 delay 1 second @23:10:40.16 AFV.select :holdingCoil @23:10:40.26 delay 1 second @23:10:41.28 AS.reconfigure ASfast @23:10:41.34 AS.seek 0ml @23:11:08.70 delay 3 seconds @23:11:11.72 AFV.select :carrierSolution @23:11:11.82 delay 2 seconds @23:11:13.84 AS.reconfigure ASconfig @23:11:13.91 AS.seek 1ml @23:12:11.68 delay 1 second @23:12:12.70 AFV.select :holdingCoil @23:12:12.80 delay 1 second @23:12:13.82 AS.reconfigure ASfast @23:12:13.88 AS.seek 0ml @23:12:41.23 delay 3 seconds @23:12:44.25 AFV.select :carrierSolution @23:12:44.35 delay 2 seconds @23:12:46.37 AS.reconfigure ASconfig @23:12:46.44 AS.seek 1ml @23:13:44.23 delay 1 second @23:13:45.25 AFV.select :holdingCoil @23:13:45.35 delay 1 second @23:13:46.37 AS.reconfigure ASnorm @23:13:46.43 AS.seek 0ml @23:14:37.17 Setting up PCR for Pp -- writing /var/log/esp/Pp19may1623h1500ml.pcr @23:14:37.18 Building PCR Slug from ASV with MM1 mix, PP2 primer @23:14:37.20 Priming with carrier @23:14:37.22 ATV.dial ARV @23:14:37.87 ARV.dial :carrier @23:14:38.23 AS.seek 0.5ml @23:15:03.75 AFV.select :carrierSolution @23:15:03.88 delay 2 seconds @23:15:05.90 AS.reconfigure ASconfig @23:15:05.97 AS.seek 1ml @23:15:35.01 delay 1 second @23:15:36.03 AFV.select :holdingCoil @23:15:36.13 delay 1 second @23:15:37.15 ATV.dial :waste @23:15:38.86 AS.reconfigure ASnorm @23:15:38.92 AS.seek :empty @23:16:29.66 AFV.select :carrierSolution @23:16:29.79 delay 2 seconds @23:16:31.81 AS.reconfigure ASconfig @23:16:31.88 AS.seek 0.5ml @23:17:01.00 delay 1 second @23:17:02.02 AFV.select :holdingCoil @23:17:02.12 Building Slug @23:17:02.14 ATV.dial :ARV @23:17:03.84 delay 2 seconds @23:17:05.86 ARV.dial :air1 @23:17:06.37 delay 2 seconds @23:17:08.44 AS.reconfigure ASslow2 @23:17:08.50 AS.seek 0.506ml @23:17:14.72 delay 2 seconds @23:17:16.74 ARV.dial :MM1 @23:17:17.36 delay 2 seconds @23:17:19.38 Adding MM1 @23:17:19.46 AS.seek 0.524ml @23:17:37.79 delay 25 seconds @23:18:02.81 ARV.dial :PP2 @23:18:03.22 Adding PP2 @23:18:03.23 delay 2 seconds @23:18:05.31 AS.seek 0.53ml @23:18:11.82 delay 10 seconds @23:18:21.85 ARV.dial :air2 @23:18:22.25 adding ARV air @23:18:22.26 delay 2 seconds @23:18:24.34 AS.seek 0.536ml @23:18:30.82 delay 5 seconds @23:18:35.84 ARV.dial :carrier @23:18:37.15 delay 7 seconds @23:18:44.23 AS.seek 0.594ml @23:19:42.76 delay 5 seconds @23:19:47.78 ATV.dial :ASV @23:19:48.18 delay 2 seconds @23:19:50.25 AS.seek 0.6ml @23:19:56.45 delay 4 seconds @23:20:00.47 ATV.dial ARV @23:20:01.12 delay 2 seconds @23:20:03.14 ARV.dial :carrier @23:20:03.17 delay 2 seconds @23:20:05.26 AS.seek 0.65ml @23:20:55.70 delay 4 seconds @23:20:59.72 ATV.dial PCR @23:21:00.36 ASV.dial :waste1 @23:21:00.37 ARV.dial :air1 @23:21:00.80 Positioning Slug by 0.324ml @23:21:00.89 AS.seek 0.326ml @23:26:27.00 delay 5 seconds @23:26:32.02 ATV.dial ARV @23:26:32.81 ASV.dial :PCRlock @23:26:33.22 delay 5 seconds @23:26:38.24 Running Pp Profile60C350 thermocycling @23:26:38.25 PCR.program Profile60C350 @23:26:38.41 Profile60C350 should complete in < 1:48:05 @23:26:38.42 PCR.run 2:20:06.25 @00:50:18.89CEST17-May-19 PCR.off @00:50:20.96 PCR for Pp completed -- closed /var/log/esp/Pp19may1623h1500ml.pcr @00:50:21.04 Cleaning PCR @00:50:21.05 AFV.select :carrierSolution @00:50:21.17 delay 2 seconds @00:50:23.19 AS.reconfigure ASconfig @00:50:23.25 AS.seek 0.5ml @00:50:33.57 delay 1 second @00:50:34.59 AFV.select :holdingCoil @00:50:34.69 ASV.dial :waste1 @00:50:35.07 ATV.dial :air3 @00:50:36.40 AS.reconfigure ASslow1 @00:50:36.46 AS.seek 0.51ml @00:50:38.76 delay 2 seconds @00:50:40.78 ATV.dial :bleach @00:50:41.42 AS.seek 0.81ml @00:51:42.01 delay 3 seconds @00:51:45.03 ATV.dial :air3 @00:51:45.61 delay 3 seconds @00:51:48.68 AS.seek 0.835ml @00:51:53.99 delay 3 seconds @00:51:57.01 ATV.dial :bleach @00:51:57.65 AS.seek 0.82ml @00:52:00.96 delay 3 seconds @00:52:03.98 ARV.dial :waste @00:52:04.61 delay 3 seconds @00:52:07.63 ATV.dial :ARV @00:52:08.55 AS.reconfigure ASconfig @00:52:08.61 AS.seek 0.47ml @00:52:29.03 AFV.select :carrierSolution @00:52:29.15 delay 2 seconds @00:52:31.18 AS.seek 1ml @00:53:01.92 delay 1 second @00:53:02.94 AFV.select :holdingCoil @00:53:03.04 delay 1 second @00:53:04.06 AS.reconfigure ASfast @00:53:04.12 AS.seek 0ml @00:53:31.44 AFV.select :carrierSolution @00:53:31.56 delay 2 seconds @00:53:33.58 AS.reconfigure ASconfig @00:53:33.64 AS.seek 1ml @00:54:31.42 delay 1 second @00:54:32.44 AFV.select :holdingCoil @00:54:32.54 delay 1 second @00:54:33.56 AS.reconfigure ASfast @00:54:33.62 AS.seek 0ml @00:55:00.95 AFV.select :carrierSolution @00:55:01.07 delay 2 seconds @00:55:03.09 AS.reconfigure ASconfig @00:55:03.15 AS.seek 1ml @00:56:00.90 delay 1 second @00:56:01.92 AFV.select :holdingCoil @00:56:02.02 delay 1 second @00:56:03.04 AS.reconfigure ASfast @00:56:03.10 AS.seek 0ml @00:56:30.45 ARV.dial between carrier and air1 @00:56:30.95 ATV.dial :air3 @00:56:32.25 AS.reconfigure ASslow1 @00:56:32.32 AS.seek 0.01ml @00:56:34.62 delay 2 seconds @00:56:36.64 ATV.dial :bleach @00:56:37.27 AS.seek 0.81ml @00:59:18.42 delay 3 seconds @00:59:21.44 ATV.dial :air3 @00:59:22.01 delay 3 seconds @00:59:25.08 AS.seek 0.835ml @00:59:30.37 delay 3 seconds @00:59:33.39 ATV.dial :bleach @00:59:34.02 AS.seek 0.82ml @00:59:37.35 delay 3 seconds @00:59:40.37 ATV.dial PCR @00:59:40.79 AS.reconfigure ASconfig @00:59:40.85 AS.seek :empty @01:00:28.32 PCR.program :bleachProfile @01:00:28.37 PCR program should complete in < 2:45 @01:00:28.39 PCR.run 10:00 @01:03:24.65 PCR.off @01:03:24.68 AFV.select :carrierSolution @01:03:24.80 delay 2 seconds @01:03:26.83 AS.seek 1ml @01:04:24.67 delay 1 second @01:04:25.69 AFV.select :holdingCoil @01:04:25.79 delay 1 second @01:04:26.81 AS.reconfigure ASfast @01:04:26.88 AS.seek 0ml @01:04:54.25 delay 3 seconds @01:04:57.27 AFV.select :carrierSolution @01:04:57.37 delay 2 seconds @01:04:59.39 AS.reconfigure ASconfig @01:04:59.46 AS.seek 1ml @01:05:57.21 delay 1 second @01:05:58.23 AFV.select :holdingCoil @01:05:58.33 delay 1 second @01:05:59.35 AS.reconfigure ASfast @01:05:59.41 AS.seek 0ml @01:06:26.76 delay 3 seconds @01:06:29.78 AFV.select :carrierSolution @01:06:29.88 delay 2 seconds @01:06:31.90 AS.reconfigure ASconfig @01:06:31.97 AS.seek 1ml @01:07:29.75 delay 1 second @01:07:30.77 AFV.select :holdingCoil @01:07:30.87 delay 1 second @01:07:31.89 AS.reconfigure ASfast @01:07:31.95 AS.seek 0ml @01:07:59.31 delay 3 seconds @01:08:02.33 AFV.select :carrierSolution @01:08:02.43 delay 2 seconds @01:08:04.45 AS.reconfigure ASconfig @01:08:04.52 AS.seek 1ml @01:09:02.29 delay 1 second @01:09:03.31 AFV.select :holdingCoil @01:09:03.41 delay 1 second @01:09:04.43 AS.reconfigure ASfast @01:09:04.49 AS.seek 0ml @01:09:31.84 delay 3 seconds @01:09:34.86 AFV.select :carrierSolution @01:09:34.96 delay 2 seconds @01:09:36.98 AS.reconfigure ASconfig @01:09:37.05 AS.seek 1ml @01:10:34.82 delay 1 second @01:10:35.84 AFV.select :holdingCoil @01:10:35.94 delay 1 second @01:10:36.96 AS.reconfigure ASnorm @01:10:37.02 AS.seek 0ml @01:11:27.77 Setting up PCR for Ss -- writing /var/log/esp/Ss19may1701h1500ml.pcr @01:11:27.78 Building PCR Slug from ASV with MM2 mix, PP4 primer @01:11:27.81 Priming with carrier @01:11:27.82 ATV.dial ARV @01:11:28.48 ARV.dial :carrier @01:11:28.82 AS.seek 0.5ml @01:11:54.41 AFV.select :carrierSolution @01:11:54.54 delay 2 seconds @01:11:56.56 AS.reconfigure ASconfig @01:11:56.63 AS.seek 1ml @01:12:25.65 delay 1 second @01:12:26.67 AFV.select :holdingCoil @01:12:26.77 delay 1 second @01:12:27.79 ATV.dial :waste @01:12:29.50 AS.reconfigure ASnorm @01:12:29.56 AS.seek :empty @01:13:20.30 AFV.select :carrierSolution @01:13:20.42 delay 2 seconds @01:13:22.44 AS.reconfigure ASconfig @01:13:22.51 AS.seek 0.5ml @01:13:51.65 delay 1 second @01:13:52.67 AFV.select :holdingCoil @01:13:52.77 Building Slug @01:13:52.78 ATV.dial :ARV @01:13:54.48 delay 2 seconds @01:13:56.50 ARV.dial :air1 @01:13:56.90 delay 2 seconds @01:13:58.97 AS.reconfigure ASslow2 @01:13:59.03 AS.seek 0.506ml @01:14:05.26 delay 2 seconds @01:14:07.28 ARV.dial :MM2 @01:14:08.78 delay 2 seconds @01:14:10.80 Adding MM2 @01:14:10.89 AS.seek 0.524ml @01:14:29.35 delay 25 seconds @01:14:54.37 ARV.dial :PP4 @01:14:54.76 Adding PP4 @01:14:54.77 delay 2 seconds @01:14:56.85 AS.seek 0.53ml @01:15:03.09 delay 10 seconds @01:15:13.12 ARV.dial :air3 @01:15:13.53 adding ARV air @01:15:13.54 delay 2 seconds @01:15:15.62 AS.seek 0.536ml @01:15:21.92 delay 5 seconds @01:15:26.94 ARV.dial :carrier @01:15:28.23 delay 7 seconds @01:15:35.31 AS.seek 0.594ml @01:16:33.85 delay 5 seconds @01:16:38.87 ATV.dial :ASV @01:16:39.29 delay 2 seconds @01:16:41.86 AS.seek 0.6ml @01:16:48.14 delay 4 seconds @01:16:52.16 ATV.dial ARV @01:16:52.82 delay 2 seconds @01:16:54.84 ARV.dial :carrier @01:16:54.85 delay 2 seconds @01:16:56.93 AS.seek 0.65ml @01:17:47.42 delay 4 seconds @01:17:51.44 ATV.dial PCR @01:17:52.07 ASV.dial :waste1 @01:17:52.08 ARV.dial :air1 @01:17:52.49 Positioning Slug by 0.324ml @01:17:52.59 AS.seek 0.326ml @01:23:18.89 delay 5 seconds @01:23:23.91 ATV.dial ARV @01:23:24.70 ASV.dial :PCRlock @01:23:25.10 delay 5 seconds @01:23:30.12 Running Ss Profile60C350 thermocycling @01:23:30.13 PCR.program Profile60C350 @01:23:30.29 Profile60C350 should complete in < 1:48:05 @01:23:30.30 PCR.run 2:20:06.25 @02:47:07.16 PCR.off @02:47:09.23 PCR for Ss completed -- closed /var/log/esp/Ss19may1701h1500ml.pcr @02:47:09.31 Cleaning PCR @02:47:09.32 AFV.select :carrierSolution @02:47:09.44 delay 2 seconds @02:47:11.46 AS.reconfigure ASconfig @02:47:11.52 AS.seek 0.5ml @02:47:21.82 delay 1 second @02:47:22.84 AFV.select :holdingCoil @02:47:22.94 ASV.dial :waste1 @02:47:23.32 ATV.dial :air3 @02:47:24.63 AS.reconfigure ASslow1 @02:47:24.69 AS.seek 0.51ml @02:47:26.97 delay 2 seconds @02:47:28.99 ATV.dial :bleach @02:47:29.62 AS.seek 0.81ml @02:48:30.23 delay 3 seconds @02:48:33.25 ATV.dial :air3 @02:48:33.81 delay 3 seconds @02:48:36.88 AS.seek 0.835ml @02:48:42.24 delay 3 seconds @02:48:45.26 ATV.dial :bleach @02:48:45.88 AS.seek 0.82ml @02:48:49.21 delay 3 seconds @02:48:52.23 ARV.dial :waste @02:48:52.85 delay 3 seconds @02:48:55.87 ATV.dial :ARV @02:48:56.78 AS.reconfigure ASconfig @02:48:56.86 AS.seek 0.47ml @02:49:17.28 AFV.select :carrierSolution @02:49:17.40 delay 2 seconds @02:49:19.43 AS.seek 1ml @02:49:50.18 delay 1 second @02:49:51.20 AFV.select :holdingCoil @02:49:51.30 delay 1 second @02:49:52.32 AS.reconfigure ASfast @02:49:52.38 AS.seek 0ml @02:50:19.72 AFV.select :carrierSolution @02:50:19.84 delay 2 seconds @02:50:21.86 AS.reconfigure ASconfig @02:50:21.92 AS.seek 1ml @02:51:19.68 delay 1 second @02:51:20.70 AFV.select :holdingCoil @02:51:20.80 delay 1 second @02:51:21.82 AS.reconfigure ASfast @02:51:21.88 AS.seek 0ml @02:51:49.25 AFV.select :carrierSolution @02:51:49.37 delay 2 seconds @02:51:51.39 AS.reconfigure ASconfig @02:51:51.45 AS.seek 1ml @02:52:49.23 delay 1 second @02:52:50.25 AFV.select :holdingCoil @02:52:50.35 delay 1 second @02:52:51.37 AS.reconfigure ASfast @02:52:51.43 AS.seek 0ml @02:53:18.77 ARV.dial between carrier and air1 @02:53:19.29 ATV.dial :air3 @02:53:20.59 AS.reconfigure ASslow1 @02:53:20.66 AS.seek 0.01ml @02:53:22.96 delay 2 seconds @02:53:24.98 ATV.dial :bleach @02:53:25.62 AS.seek 0.81ml @02:56:06.76 delay 3 seconds @02:56:09.78 ATV.dial :air3 @02:56:10.34 delay 3 seconds @02:56:13.41 AS.seek 0.835ml @02:56:18.72 delay 3 seconds @02:56:21.74 ATV.dial :bleach @02:56:22.37 AS.seek 0.82ml @02:56:25.70 delay 3 seconds @02:56:28.72 ATV.dial PCR @02:56:29.14 AS.reconfigure ASconfig @02:56:29.20 AS.seek :empty @02:57:16.68 PCR.program :bleachProfile @02:57:16.73 PCR program should complete in < 2:45 @02:57:16.74 PCR.run 10:00 @03:00:13.01 PCR.off @03:00:13.04 AFV.select :carrierSolution @03:00:13.16 delay 2 seconds @03:00:15.19 AS.seek 1ml @03:01:13.03 delay 1 second @03:01:14.05 AFV.select :holdingCoil @03:01:14.15 delay 1 second @03:01:15.17 AS.reconfigure ASfast @03:01:15.23 AS.seek 0ml @03:01:42.57 delay 3 seconds @03:01:45.59 AFV.select :carrierSolution @03:01:45.69 delay 2 seconds @03:01:47.71 AS.reconfigure ASconfig @03:01:47.78 AS.seek 1ml @03:02:45.56 delay 1 second @03:02:46.58 AFV.select :holdingCoil @03:02:46.68 delay 1 second @03:02:47.70 AS.reconfigure ASfast @03:02:47.76 AS.seek 0ml @03:03:15.10 delay 3 seconds @03:03:18.12 AFV.select :carrierSolution @03:03:18.22 delay 2 seconds @03:03:20.24 AS.reconfigure ASconfig @03:03:20.30 AS.seek 1ml @03:04:18.07 delay 1 second @03:04:19.09 AFV.select :holdingCoil @03:04:19.19 delay 1 second @03:04:20.21 AS.reconfigure ASfast @03:04:20.27 AS.seek 0ml @03:04:47.62 delay 3 seconds @03:04:50.64 AFV.select :carrierSolution @03:04:50.74 delay 2 seconds @03:04:52.76 AS.reconfigure ASconfig @03:04:52.83 AS.seek 1ml @03:05:50.64 delay 1 second @03:05:51.66 AFV.select :holdingCoil @03:05:51.76 delay 1 second @03:05:52.78 AS.reconfigure ASfast @03:05:52.84 AS.seek 0ml @03:06:20.18 delay 3 seconds @03:06:23.20 AFV.select :carrierSolution @03:06:23.30 delay 2 seconds @03:06:25.32 AS.reconfigure ASconfig @03:06:25.39 AS.seek 1ml @03:07:23.18 delay 1 second @03:07:24.20 AFV.select :holdingCoil @03:07:24.30 delay 1 second @03:07:25.32 AS.reconfigure ASnorm @03:07:25.38 AS.seek 0ml @03:08:16.14 Setting up PCR for Mm -- writing /var/log/esp/Mm19may1703h1500ml.pcr @03:08:16.15 Building PCR Slug from ASV with MM2 mix, PP3 primer @03:08:16.17 Priming with carrier @03:08:16.19 ATV.dial ARV @03:08:16.85 ARV.dial :carrier @03:08:17.21 AS.seek 0.5ml @03:08:42.73 AFV.select :carrierSolution @03:08:42.86 delay 2 seconds @03:08:44.88 AS.reconfigure ASconfig @03:08:44.95 AS.seek 1ml @03:09:13.96 delay 1 second @03:09:14.98 AFV.select :holdingCoil @03:09:15.08 delay 1 second @03:09:16.10 ATV.dial :waste @03:09:17.81 AS.reconfigure ASnorm @03:09:17.87 AS.seek :empty @03:10:08.63 AFV.select :carrierSolution @03:10:08.75 delay 2 seconds @03:10:10.77 AS.reconfigure ASconfig @03:10:10.84 AS.seek 0.5ml @03:10:39.96 delay 1 second @03:10:40.98 AFV.select :holdingCoil @03:10:41.08 Building Slug @03:10:41.09 ATV.dial :ARV @03:10:42.89 delay 2 seconds @03:10:44.91 ARV.dial :air1 @03:10:45.32 delay 2 seconds @03:10:47.39 AS.reconfigure ASslow2 @03:10:47.45 AS.seek 0.506ml @03:10:53.84 delay 2 seconds @03:10:55.86 ARV.dial :MM2 @03:10:57.39 delay 2 seconds @03:10:59.41 Adding MM2 @03:10:59.49 AS.seek 0.524ml @03:11:17.96 delay 25 seconds @03:11:42.98 ARV.dial :PP3 @03:11:43.39 Adding PP3 @03:11:43.40 delay 2 seconds @03:11:45.48 AS.seek 0.53ml @03:11:51.68 delay 10 seconds @03:12:01.71 ARV.dial :air2 @03:12:02.12 adding ARV air @03:12:02.13 delay 2 seconds @03:12:04.21 AS.seek 0.536ml @03:12:10.43 delay 5 seconds @03:12:15.45 ARV.dial :carrier @03:12:16.74 delay 7 seconds @03:12:23.82 AS.seek 0.594ml @03:13:22.37 delay 5 seconds @03:13:27.39 ATV.dial :ASV @03:13:27.81 delay 2 seconds @03:13:29.88 AS.seek 0.6ml @03:13:36.65 delay 4 seconds @03:13:40.67 ATV.dial ARV @03:13:41.21 delay 2 seconds @03:13:43.23 ARV.dial :carrier @03:13:43.24 delay 2 seconds @03:13:45.32 AS.seek 0.65ml @03:14:35.95 delay 4 seconds @03:14:39.97 ATV.dial PCR @03:14:40.60 ASV.dial :waste1 @03:14:40.61 ARV.dial :air1 @03:14:41.13 Positioning Slug by 0.324ml @03:14:41.22 AS.seek 0.326ml @03:20:07.53 delay 5 seconds @03:20:12.55 ATV.dial ARV @03:20:13.34 ASV.dial :PCRlock @03:20:13.74 delay 5 seconds @03:20:18.76 Running Mm Profile60C350 thermocycling @03:20:18.78 PCR.program Profile60C350 @03:20:18.93 Profile60C350 should complete in < 1:48:05 @03:20:18.94 PCR.run 2:20:06.25 @04:12:59.31 Session Ended @04:43:59.12 PCR.off @04:44:01.19 PCR for Mm completed -- closed /var/log/esp/Mm19may1703h1500ml.pcr @04:44:01.20 Full MFB Decontamination @04:44:01.21 Decon extraction column and decon AIV @04:44:01.23 SPE.hold 2:46:40,55C @04:44:01.27 ASV.dial :SPEdelivery @04:44:01.93 delay 20 seconds @04:44:21.96 AFV.select :carrierSolution @04:44:22.06 delay 2 seconds @04:44:24.08 AS.reconfigure ASconfig @04:44:24.13 AS.seek 0.5ml @04:44:34.42 delay 1 second @04:44:35.44 AFV.select :holdingCoil @04:44:35.54 ATV.dial :air1 @04:44:37.74 AS.reconfigure ASslow1 @04:44:37.78 AS.seek 0.52ml @04:44:42.09 ATV.dial :ASV @04:44:43.79 ASV.dial :SPErecovery @04:44:44.46 AS.seek 0.62ml @04:45:04.94 ATV.dial :waste @04:45:07.06 AS.reconfigure ASnorm @04:45:07.11 AS.seek :empty @04:45:38.72 AFV.select :holdingCoil @04:45:38.74 ATV.dial :waste @04:45:38.75 AS.reconfigure ASconfig @04:45:38.79 AS.seek 0ml @04:45:39.05 AFV.select :carrierSolution @04:45:39.16 delay 2 seconds @04:45:41.19 AS.seek 0.5ml @04:46:10.20 delay 1 second @04:46:11.22 AFV.select :holdingCoil @04:46:11.32 ATV.dial :air3 @04:46:12.07 AS.reconfigure ASslow1 @04:46:12.11 AS.seek 0.52ml @04:46:16.35 ATV.dial :air3 @04:46:16.42 AS.seek 0.53ml @04:46:18.74 delay 2 seconds @04:46:20.76 ATV.dial :bleach @04:46:21.24 AS.seek 0.71ml @04:46:57.71 delay 3 seconds @04:47:00.73 ATV.dial :air3 @04:47:01.32 delay 3 seconds @04:47:04.39 AS.seek 0.735ml @04:47:09.71 delay 3 seconds @04:47:12.73 ATV.dial :bleach @04:47:13.38 AS.seek 0.72ml @04:47:16.68 delay 3 seconds @04:47:19.70 ATV.dial ASV @04:47:20.82 ASV.dial :SPErecovery @04:47:20.89 AS.seek 0.365ml @04:48:32.53 ATV.dial :waste @04:48:34.35 AS.reconfigure ASnorm @04:48:34.39 AS.seek :empty @04:48:53.18 ATV.dial :air3 @04:48:53.88 AS.reconfigure ASslow1 @04:48:53.92 AS.seek 0.009ml @04:48:56.23 delay 2 seconds @04:48:58.25 ATV.dial :bleach @04:48:58.73 AS.seek 0.579ml @04:50:53.64 delay 3 seconds @04:50:56.66 ATV.dial :air3 @04:50:57.23 delay 3 seconds @04:51:00.30 AS.seek 0.604ml @04:51:05.62 delay 3 seconds @04:51:08.65 ATV.dial :bleach @04:51:09.27 AS.seek 0.589ml @04:51:12.57 delay 3 seconds @04:51:15.59 ATV.dial :ASV @04:51:16.64 ASV.dial :wasteR @04:51:17.51 AS.seek 0ml @04:53:16.28 delay 1:00 @04:54:16.31 ATV.dial ASV @04:54:16.32 AFV.select :carrierSolution @04:54:16.42 delay 2 seconds @04:54:18.44 AS.reconfigure ASconfig @04:54:18.49 AS.seek 1ml @04:55:16.24 delay 1 second @04:55:17.26 AFV.select :holdingCoil @04:55:17.36 delay 1 second @04:55:18.38 ASV.dial :wasteR @04:55:18.39 AS.reconfigure ASslow1 @04:55:18.44 AS.seek 0ml @04:58:39.82 delay 10 seconds @04:58:49.85 ATV.dial :air3 @04:58:51.40 AS.seek 0.01ml @04:58:53.70 delay 2 seconds @04:58:55.72 ATV.dial :bleach @04:58:56.34 AS.seek 0.58ml @05:00:51.23 delay 3 seconds @05:00:54.25 ATV.dial :air3 @05:00:54.81 delay 3 seconds @05:00:57.88 AS.seek 0.605ml @05:01:03.15 delay 3 seconds @05:01:06.17 ATV.dial :bleach @05:01:06.79 AS.seek 0.59ml @05:01:10.09 delay 3 seconds @05:01:13.11 ATV.dial ASV @05:01:14.17 ASV.dial :SPEdelivery @05:01:14.62 AS.seek 0ml @05:03:13.54 delay 1:00 @05:04:13.57 AFV.select :carrierSolution @05:04:13.67 delay 2 seconds @05:04:15.69 AS.reconfigure ASconfig @05:04:15.74 AS.seek 1ml @05:05:13.51 delay 1 second @05:05:14.53 AFV.select :holdingCoil @05:05:14.63 delay 1 second @05:05:15.65 ATV.dial ASV @05:05:15.66 ASV.dial :SPEdelivery @05:05:15.68 AS.reconfigure ASslow1 @05:05:15.72 AS.seek 0ml @05:08:37.10 delay 10 seconds @05:08:47.13 ATV.dial :air2 @05:08:47.97 AS.reconfigure ASslow0 @05:08:48.01 AS.seek 0.8ml @05:10:08.76 delay 3 seconds @05:10:11.78 ATV.dial :ASV @05:10:12.78 ASV.dial :SPEdelivery @05:10:12.80 AS.reconfigure ASslow1 @05:10:12.84 AS.seek 0.03ml @05:12:47.98 delay 20 seconds @05:13:08.01 ATV.dial :waste,via: :air3 @05:13:09.78 AS.reconfigure ASconfig @05:13:09.82 AS.seek :empty @05:13:11.88 SPE.coast @05:13:11.95 Removing Bleach from Mixing Coil @05:13:11.96 AFV.select :carrierSolution @05:13:12.06 delay 2 seconds @05:13:14.09 AS.seek 0.5ml @05:13:43.18 delay 1 second @05:13:44.20 AFV.select :holdingCoil @05:13:44.30 ATV.dial :ASV @05:13:46.04 ASV.dial :SPErecovery @05:13:46.68 AS.reconfigure ASslow1 @05:13:46.72 AS.seek 0.73ml @05:14:33.27 ASV.dial :wasteD @05:14:33.84 AS.reconfigure ASnorm @05:14:33.89 AS.seek 0ml @05:15:10.97 AFV.select :carrierSolution @05:15:11.08 delay 2 seconds @05:15:13.10 AS.reconfigure ASconfig @05:15:13.15 AS.seek 0.5ml @05:15:42.18 delay 1 second @05:15:43.20 AFV.select :holdingCoil @05:15:43.30 ATV.dial ASV @05:15:43.31 ASV.dial :SPErecovery @05:15:43.94 AS.reconfigure ASslow0 @05:15:43.99 AS.seek 0.3ml @05:16:04.39 ATV.dial :air2 @05:16:05.45 AS.reconfigure ASslow1 @05:16:05.49 AS.seek 0.32ml @05:16:09.84 ATV.dial ASV @05:16:10.89 AS.reconfigure ASslow0 @05:16:10.94 AS.seek 0.57ml @05:16:36.42 ASV.dial :wasteD @05:16:37.01 AS.reconfigure ASnorm @05:16:37.06 AS.seek 0ml @05:17:06.11 AFV.select :carrierSolution @05:17:06.22 delay 2 seconds @05:17:08.24 AS.reconfigure ASconfig @05:17:08.29 AS.seek 0.5ml @05:17:37.35 delay 1 second @05:17:38.37 AFV.select :holdingCoil @05:17:38.47 ATV.dial ASV @05:17:38.48 ASV.dial :SPErecovery @05:17:39.62 AS.reconfigure ASslow0 @05:17:39.67 AS.seek 0.3ml @05:18:00.09 ATV.dial :air2 @05:18:01.13 AS.reconfigure ASslow1 @05:18:01.17 AS.seek 0.32ml @05:18:05.49 ATV.dial ASV @05:18:06.54 AS.reconfigure ASslow0 @05:18:06.58 AS.seek 0.57ml @05:18:32.05 ASV.dial :wasteD @05:18:32.71 AS.reconfigure ASnorm @05:18:32.76 AS.seek 0ml @05:19:01.82 ATV.dial :air2 @05:19:02.81 AS.reconfigure ASslow0 @05:19:02.86 AS.seek 0.3ml @05:19:33.42 ATV.dial :ASV @05:19:34.40 ASV.dial :wasteD @05:19:34.41 AS.reconfigure ASconfig @05:19:34.46 AS.seek 0.05ml @05:19:49.13 ATV.dial :waste @05:19:51.03 AS.seek 0ml @05:19:54.18 ASV.dial :SPEdelivery @05:19:55.17 deconLysateINT @05:19:55.18 ATV.dial :air3 @05:19:55.87 AS.reconfigure ASslow1 @05:19:55.91 AS.seek 0.01ml @05:19:58.24 delay 2 seconds @05:20:00.26 ATV.dial :bleach @05:20:00.71 AS.seek 0.96ml @05:23:12.01 delay 3 seconds @05:23:15.03 ATV.dial :air3 @05:23:15.57 delay 3 seconds @05:23:18.64 AS.seek 0.985ml @05:23:23.93 delay 3 seconds @05:23:26.96 ATV.dial :bleach @05:23:27.57 AS.seek 0.97ml @05:23:30.87 delay 3 seconds @05:23:33.89 ATV.dial AIV @05:23:35.15 AIV.dial :coilToMFB @05:23:35.16 AWV.select :waste @05:23:35.17 AFV.select :carrierSolution @05:23:35.27 delay 2 seconds @05:23:37.29 AS.reconfigure ASconfig @05:23:37.34 AS.seek 1ml @05:23:39.38 delay 1 second @05:23:40.40 AFV.select :holdingCoil @05:23:40.50 delay 1 second @05:23:41.52 AS.reconfigure ASslow0 @05:23:41.57 AS.seek :empty @05:25:22.52 AFV.select :carrierSolution @05:25:22.63 delay 2 seconds @05:25:24.65 AS.reconfigure ASconfig @05:25:24.70 AS.seek 1ml @05:26:22.51 delay 1 second @05:26:23.53 AFV.select :holdingCoil @05:26:23.63 delay 1 second @05:26:24.65 AS.reconfigure ASslow0 @05:26:24.70 AS.seek :empty @05:28:05.64 AFV.select :carrierSolution @05:28:05.75 delay 2 seconds @05:28:07.77 AS.reconfigure ASconfig @05:28:07.82 AS.seek 1ml @05:29:05.65 delay 1 second @05:29:06.67 AFV.select :holdingCoil @05:29:06.77 delay 1 second @05:29:07.79 AS.reconfigure ASslow0 @05:29:07.84 AS.seek :empty @05:30:48.78 ATV.dial :air2 @05:30:49.46 AS.reconfigure ASslow1 @05:30:49.50 AS.seek 0.009ml @05:30:51.84 AS.reconfigure ASslow0 @05:30:51.88 AS.seek 1ml @05:32:31.73 ATV.dial AIV @05:32:32.51 AS.seek 0.02ml @05:34:11.37 ATV.dial :waste @05:34:13.04 AS.seek 0ml @05:34:15.37 AWV.select :air @05:34:15.53 Cleaning PCR @05:34:15.54 AFV.select :carrierSolution @05:34:15.63 delay 2 seconds @05:34:17.65 AS.reconfigure ASconfig @05:34:17.70 AS.seek 0.5ml @05:34:46.74 delay 1 second @05:34:47.76 AFV.select :holdingCoil @05:34:47.86 ASV.dial :waste1 @05:34:48.23 ATV.dial :air3 @05:34:48.90 AS.reconfigure ASslow1 @05:34:48.95 AS.seek 0.51ml @05:34:51.35 delay 2 seconds @05:34:53.37 ATV.dial :bleach @05:34:54.32 AS.seek 0.81ml @05:35:55.03 delay 3 seconds @05:35:58.05 ATV.dial :air3 @05:35:58.62 delay 3 seconds @05:36:01.69 AS.seek 0.835ml @05:36:07.04 delay 3 seconds @05:36:10.06 ATV.dial :bleach @05:36:10.66 AS.seek 0.82ml @05:36:13.98 delay 3 seconds @05:36:17.00 ARV.dial :waste @05:36:17.60 delay 3 seconds @05:36:20.62 ATV.dial :ARV @05:36:21.51 AS.reconfigure ASconfig @05:36:21.55 AS.seek 0.47ml @05:36:41.97 AFV.select :carrierSolution @05:36:42.08 delay 2 seconds @05:36:44.11 AS.seek 1ml @05:37:14.88 delay 1 second @05:37:15.90 AFV.select :holdingCoil @05:37:16.00 delay 1 second @05:37:17.02 AS.reconfigure ASfast @05:37:17.07 AS.seek 0ml @05:37:44.41 AFV.select :carrierSolution @05:37:44.52 delay 2 seconds @05:37:46.54 AS.reconfigure ASconfig @05:37:46.58 AS.seek 1ml @05:38:44.32 delay 1 second @05:38:45.34 AFV.select :holdingCoil @05:38:45.44 delay 1 second @05:38:46.46 AS.reconfigure ASfast @05:38:46.51 AS.seek 0ml @05:39:13.81 AFV.select :carrierSolution @05:39:13.92 delay 2 seconds @05:39:15.94 AS.reconfigure ASconfig @05:39:15.99 AS.seek 1ml @05:40:13.74 delay 1 second @05:40:14.76 AFV.select :holdingCoil @05:40:14.86 delay 1 second @05:40:15.88 AS.reconfigure ASfast @05:40:15.93 AS.seek 0ml @05:40:43.28 ARV.dial between carrier and air1 @05:40:43.78 ATV.dial :air3 @05:40:45.05 AS.reconfigure ASslow1 @05:40:45.09 AS.seek 0.01ml @05:40:47.38 delay 2 seconds @05:40:49.40 ATV.dial :bleach @05:40:50.01 AS.seek 0.81ml @05:43:31.13 delay 3 seconds @05:43:34.15 ATV.dial :air3 @05:43:34.70 delay 3 seconds @05:43:37.77 AS.seek 0.835ml @05:43:43.08 delay 3 seconds @05:43:46.10 ATV.dial :bleach @05:43:46.71 AS.seek 0.82ml @05:43:50.03 delay 3 seconds @05:43:53.05 ATV.dial PCR @05:43:53.46 AS.reconfigure ASconfig @05:43:53.50 AS.seek :empty @05:44:40.96 PCR.program :bleachProfile @05:44:41.01 PCR program should complete in < 2:45 @05:44:41.02 PCR.run 10:00 @05:47:39.81 PCR.off @05:47:39.83 AFV.select :carrierSolution @05:47:39.93 delay 2 seconds @05:47:41.96 AS.seek 1ml @05:48:39.81 delay 1 second @05:48:40.83 AFV.select :holdingCoil @05:48:40.93 delay 1 second @05:48:41.95 AS.reconfigure ASfast @05:48:42.00 AS.seek 0ml @05:49:09.35 delay 3 seconds @05:49:12.37 AFV.select :carrierSolution @05:49:12.47 delay 2 seconds @05:49:14.49 AS.reconfigure ASconfig @05:49:14.55 AS.seek 1ml @05:50:12.31 delay 1 second @05:50:13.33 AFV.select :holdingCoil @05:50:13.43 delay 1 second @05:50:14.45 AS.reconfigure ASfast @05:50:14.50 AS.seek 0ml @05:50:41.85 delay 3 seconds @05:50:44.87 AFV.select :carrierSolution @05:50:44.97 delay 2 seconds @05:50:46.99 AS.reconfigure ASconfig @05:50:47.05 AS.seek 1ml @05:51:44.82 delay 1 second @05:51:45.84 AFV.select :holdingCoil @05:51:45.94 delay 1 second @05:51:46.96 AS.reconfigure ASfast @05:51:47.01 AS.seek 0ml @05:52:14.35 delay 3 seconds @05:52:17.37 AFV.select :carrierSolution @05:52:17.47 delay 2 seconds @05:52:19.49 AS.reconfigure ASconfig @05:52:19.55 AS.seek 1ml @05:53:17.35 delay 1 second @05:53:18.37 AFV.select :holdingCoil @05:53:18.47 delay 1 second @05:53:19.49 AS.reconfigure ASfast @05:53:19.54 AS.seek 0ml @05:53:46.88 delay 3 seconds @05:53:49.90 AFV.select :carrierSolution @05:53:50.00 delay 2 seconds @05:53:52.02 AS.reconfigure ASconfig @05:53:52.08 AS.seek 1ml @05:54:49.84 delay 1 second @05:54:50.86 AFV.select :holdingCoil @05:54:50.96 delay 1 second @05:54:51.98 AS.reconfigure ASnorm @05:54:52.03 AS.seek 0ml @05:55:42.72 AFV.select :carrierSolution @05:55:42.82 delay 2 seconds @05:55:44.84 AS.reconfigure ASconfig @05:55:44.89 AS.seek 1ml @05:56:42.68 delay 1 second @05:56:43.70 AFV.select :holdingCoil @05:56:43.80 delay 1 second @05:56:44.82 ATV.dial :waste @05:56:46.06 AS.reconfigure ASnorm @05:56:46.11 AS.seek 0.5ml @05:57:11.67 ATV.dial :waste2 @05:57:12.98 AS.seek 0ml @05:57:38.54
Asleep after completing 190516totalsampletest assays @05:57:38.55 GoodNews.email "{1 => 3, 2 => 0}\nCan@05:48:08, 21.8C, 30% humidity, 14.6psia, 12.060V, 0.474A, 0.474A avg, 5.72W",Subject: "Asleep after completing 190516totalsampletest assays" @05:57:38.57
Mission runtime was 11:39:30.105698 @16:09:16.10CEST17-May-19
>RESET< ESP2 v3.30 I2C Gateway v3.06 for core bus @16:09:17.82 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @16:09:18.08 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @16:09:22.50 Polling Can every 10:00 @16:09:22.52 Cleanly exited at 15:56:03CEST17-May-19 @16:09:22.55 Loading /home/esp/esp2/mission/190517totalsampletest.rb mission @16:09:22.59 RuntimeError in MAIN -- Mission's startTube should be 3, not 2 @16:09:22.61 Waiting to Resume from Checkpoint "Loading /home/esp/esp2/mission/190517totalsampletest.rb mission" @16:09:22.63 BadNews.email "Mission's startTube should be 3, not 2",Subject: "RuntimeError in MAIN" @16:14:39.86CEST17-May-19 LoadError in MAIN -- no such file to load -- i2c/sleepy @16:14:39.88 SystemExit in MAIN -- exit @16:23:44.65CEST17-May-19
ESP2 v3.30 I2C Gateway v3.06 for core bus @16:23:46.33 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @16:23:46.57 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @16:23:50.88 Polling Can every 10:00 @16:23:50.92 Loading /home/esp/esp2/mission/190517totalsampletest.rb mission @16:23:50.99 Preparing to sleep until +0:00:15 @16:23:51.00 GoodNews.email "Starting 190517totalsampletest w/pucks in tube 2\n{}\nCan@16:23:50, 21.8C, 39% humidity, 14.6psia, 12.164V, 0.096A, 0.090A avg, 1.17W",Subject: "190517totalsampletest begun" @16:23:51.08 Shutdown @16:23:51.09
Asleep til +0:00:15 @16:23:51.11 Continuing at 16:24 @16:23:56.88 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/190517totalsampletest.rb:111\n{}\nCan@16:23:51, 21.8C, 39% humidity, 14.6psia, 12.164V, 0.096A, 0.096A avg, 1.17W",Subject: "Asleep until 16:24" @16:24:05.96
Powering core bus @16:24:10.85 Gate.power :raw,:ON @16:24:11.41 SPE.coast @16:24:11.47 SPE.configure SPEconfig @16:24:11.55 AS.configure ASconfig @16:24:11.64 SS.configure SSconfig @16:24:11.72 CC.configure CCconfig @16:24:11.80 CS.configure CSconfig @16:24:11.89 PC.configure PCconfig @16:24:11.97 PS.configure PSconfig @16:24:12.08 Forearm.configure LooseForearm @16:24:12.16 Elbow.configure LooseElbow @16:24:12.26 SC.configure SCloose @16:24:12.34 SE.configure SEconfig @16:24:12.38 CH.coast @16:24:12.43 CH.configure CHconfig @16:24:12.46 PH.coast @16:24:12.51 PH.configure PHconfig @16:24:12.54 PCR.off @16:24:12.57 PCR.pidtemp= 50,0.1,0,300,25,25 @16:24:12.60 PCR.caltemp= 2.8814,0.0372,0 @16:24:12.63 All dwarf controllers running firmware version 3.75 @16:24:12.74 Valve.reset @16:24:12.90 PCR.off @16:24:12.92 PCR.pidtemp= 50,0.1,0,300,25,25 @16:24:12.95 PCR.caltemp= 2.8814,0.0372,0 @16:24:13.11 Intake.select :close @16:24:13.13 Exhaust.select :close @16:24:13.15 CTV.dial :air @16:24:13.26 PSV.dial :air @16:24:13.40 CSV.dial :waste1 @16:24:13.43
Forearm.reconfigure LooseForearm @16:24:13.45 PS.reconfigure PSconfig @16:24:13.57
Forearm.home.seek :retract @16:24:13.60 PS.home.seek 0ml @16:24:13.68 SSV.dial :spit,avoiding: :exhaust @16:24:13.80 CS.reconfigure CSfast @16:24:13.83 SS.reconfigure SSconfig @16:24:13.91 CS.home.seek 0ml @16:24:13.94 SS.home.seek 0ml @16:24:14.30 Hand.close @16:24:14.32
Elbow.jog 1500 @16:24:14.79 Elbow.reconfigure LooseElbow @16:24:14.83 Elbow.home.seek :clear @16:24:19.49 SE.reconfigure SEconfig @16:24:19.53 SE.home.seek :down @16:24:20.24 SC.reconfigure SCsloppy @16:24:20.28 SC.home.seek :initial @16:24:22.76 PS.coast @16:24:30.53
Forearm.seek :retract @16:24:30.60 Elbow.seek :hammer @16:24:31.74 Forearm.reconfigure TightForearm @16:24:31.78 Forearm.seek 5 + 350 counts @16:24:32.80 Forearm.seek :retract @16:24:33.99 PC.reconfigure PCconfig @16:24:34.01
CC.reconfigure CCconfig @16:24:34.09 CC.home.seek :open @16:24:34.11 PC.home.seek :open @16:24:37.40
Phase [mbaPhase] @16:24:37.41 GoodNews.email "At 16:24:37 began in tube 2: mbaPhase\n/home/esp/esp2/mission/190517totalsampletest.rb:111\n{}\nCan@16:24:37, 21.8C, 39% humidity, 14.6psia, 11.977V, 0.790A, 0.790A avg, 9.46W",Subject: "Started 190517totalsampletest phase mbaPhase" @16:24:37.44CEST17-May-19
==================> STARTED MBAsuperphase (phase #1) <=== @16:24:38.03 Readying the MFB @16:24:38.04 Homing MFB Syringe @16:24:38.05 AFV.select :holdingCoil @16:24:38.18 ATV.dial :air2 @16:24:38.77 AS.reconfigure ASconfig @16:24:38.81 AS.home.seek :home @16:24:44.34 AFV.select :carrierSolution @16:24:44.44 delay 2 seconds @16:24:46.47 AS.seek 1ml @16:25:39.34 delay 1 second @16:25:40.36 AFV.select :holdingCoil @16:25:40.46 delay 1 second @16:25:41.48 ATV.dial :waste @16:25:42.57 AS.reconfigure ASnorm @16:25:42.61 AS.home.seek :empty @16:26:35.18 ATV.dial :waste @16:26:35.19 ARV.dial between carrier and air1 @16:26:35.27 ASV.dial :waste1 @16:26:35.42 Starting mfb @16:26:35.43 pcrBAC sampling at most 1000ml, wcr at most 1500ml @16:26:35.47 BACfans.email "Started mfb in tube 2 at 16:26:35CEST17-May-19\n{}\nCan@16:24:37, 21.8C, 39% humidity, 14.6psia, 11.977V, 0.790A, 0.790A avg, 9.46W",Subject: "pcrBAC sampling at most 1000ml, wcr at most 1500ml" @16:26:35.53
Loading sh1 puck from tube 2 into Collection Clamp @16:26:35.60 Hand.open @16:26:36.57 SC.reconfigure SCloose @16:26:36.71 Elbow.reconfigure TightElbow @16:26:36.75 Elbow.seek 2 @16:26:37.26 Forearm.seek 2 + 700 counts @16:26:38.33 SE.reconfigure SEpick @16:26:38.40 SE.seek beamBreak @16:26:41.39 SE.configure SEconfig @16:26:41.54 Elbow.seek 2 @16:26:41.71 Forearm.seek 2 + 300 counts @16:26:41.88 Forearm.reconfigure LooseForearm @16:26:41.92 Hand.close @16:26:42.62 SE.seek :down @16:26:42.82
Forearm.seek :retract @16:26:43.78 Elbow.seek CC @16:26:45.32 SE.coast @16:26:45.89
Elbow.reconfigure LooseElbow @16:26:45.99 Forearm.reconfigure TightForearm @16:26:46.03 Forearm.seek CC @16:26:47.34 CC.seek :closed @16:26:51.55 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.52 @16:26:51.56 Hand.open @16:26:52.41 Forearm.reconfigure LooseForearm @16:26:52.45 Forearm.seek :retract @16:26:53.48 SC.coast @16:26:53.51 collectSample 1000ml w/bubble point of 55psi @16:26:53.52 Priming 7ml @16:26:53.53 Gate.power :sampler,:ON @16:26:53.54 Intake.select :close @16:26:53.55 Exhaust.select :close @16:26:53.56 SS.hold @16:26:53.75 Calibrating pressure and temperature sensors @16:26:53.77 CTV.dial :air @16:26:53.78 SSV.dial :spit,avoiding: :exhaust @16:26:53.80 SS.reconfigure SSfast @16:26:53.84 SS.seek 5ml @16:26:56.46 SSV.dial CBV @16:26:56.49
CBV.dial SSV @16:26:57.97 delay 3.8 seconds @16:27:01.89 SSV.dial :spit @16:27:03.38 SS.seek 0ml @16:27:06.36 SSV.dial CBV @16:27:07.82 CTV.dial :intake @16:27:08.69 Waiting for external sampler to finish priming... @16:27:08.70 delayUntil "16:31:53.54" @16:31:53.55 SSV.dial CBV @16:31:53.58
CBV.dial SSV @16:31:53.59 Allowing 40s for 7.0ml of remaining 7.0ml @16:31:53.60 Intake.select :open @16:31:55.75 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:31:55.76 SS.reconfigure SSinhale.with maxSpeed: 150 @16:31:55.80 SS.seek 7ml @16:32:01.81 Stroke completed -- waiting for pressure to equalize ... @16:32:16.40 Intake.select :close @16:32:16.54 SSV.dial :exhaust @16:32:17.37 Exhaust.select :open @16:32:17.48 SS.reconfigure SSexhale @16:32:17.53 SS.seek 0ml @16:32:21.71 Exhaust.select :close @16:32:21.82 Sampling 1000.0ml @16:32:21.84 Intake.select :close @16:32:21.85 Exhaust.select :close @16:32:21.86 SS.reconfigure SSconfig @16:32:21.90 SS.hold @16:32:22.09 CTV.dial :intake @16:32:22.52 SSV.dial CBV @16:32:22.55
CBV.dial SSV @16:32:23.42 Allowing 130s for 25.0ml of remaining 1000.0ml @16:32:23.43 Intake.select :open @16:32:25.59 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:32:25.60 SS.reconfigure SSinhale.with maxSpeed: 150 @16:32:25.65 SS.seek 25ml @16:32:29.94 Vacuum too high -- waiting up to 40s @16:32:36.69 Intake @15.4psia, vacuum from 8.9 to 12.9psi @16:32:36.70 SS.reconfigure SSinhale.with maxSpeed: 135 @16:32:36.74 SS.seek 25ml @16:32:40.35 Vacuum too high -- waiting up to 40s @16:32:47.97 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:32:47.98 SS.reconfigure SSinhale.with maxSpeed: 122 @16:32:48.03 SS.seek 25ml @16:32:51.41 Vacuum too high -- waiting up to 40s @16:32:57.92 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:32:57.93 SS.reconfigure SSinhale.with maxSpeed: 110 @16:32:57.98 SS.seek 25ml @16:33:01.83 Vacuum too high -- waiting up to 40s @16:33:08.89 SS.reconfigure SSinhale.with maxSpeed: 99 @16:33:08.93 SS.seek 25ml @16:33:13.24 Stroke completed -- waiting for pressure to equalize ... @16:33:21.17 Intake.select :close @16:33:21.31 SSV.dial :exhaust @16:33:22.17 Exhaust.select :open @16:33:22.28 SS.reconfigure SSexhale @16:33:22.33 SS.seek 0ml @16:33:32.09 Exhaust.select :close @16:33:32.20 SSV.dial CBV @16:33:32.22
CBV.dial SSV @16:33:33.09 Allowing 130s for 25.0ml of remaining 975.0ml @16:33:33.10 Intake.select :open @16:33:35.26 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:33:35.27 SS.reconfigure SSinhale.with maxSpeed: 99 @16:33:35.31 SS.seek 25ml @16:33:39.57 Vacuum too high -- waiting up to 40s @16:33:44.04 Intake @15.4psia, vacuum from 8.9 to 12.9psi @16:33:44.05 SS.reconfigure SSinhale.with maxSpeed: 89 @16:33:44.09 SS.seek 25ml @16:33:48.04 Vacuum too high -- waiting up to 40s @16:33:52.91 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:33:52.92 SS.reconfigure SSinhale.with maxSpeed: 80 @16:33:52.96 SS.seek 25ml @16:33:57.60 Vacuum too high -- waiting up to 40s @16:34:02.50 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:34:02.52 SS.reconfigure SSinhale.with maxSpeed: 72 @16:34:02.56 SS.seek 25ml @16:34:08.22 Vacuum too high -- waiting up to 40s @16:34:13.58 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:34:13.60 SS.reconfigure SSinhale.with maxSpeed: 65 @16:34:13.64 SS.seek 25ml @16:34:19.22 Vacuum too high -- waiting up to 40s @16:34:23.85 SS.reconfigure SSinhale.with maxSpeed: 59 @16:34:23.90 SS.seek 25ml @16:34:27.92 Stroke completed -- waiting for pressure to equalize ... @16:34:32.22 Intake.select :close @16:34:32.35 SSV.dial :exhaust @16:34:33.20 Exhaust.select :open @16:34:33.31 SS.reconfigure SSexhale @16:34:33.36 SS.seek 0ml @16:34:42.82 Exhaust.select :close @16:34:42.93 SSV.dial CBV @16:34:42.95
CBV.dial SSV @16:34:43.81 Allowing 130s for 25.0ml of remaining 950.0ml @16:34:43.82 Intake.select :open @16:34:45.98 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:34:45.99 SS.reconfigure SSinhale.with maxSpeed: 59 @16:34:46.03 SS.seek 25ml @16:34:53.60 Vacuum too high -- waiting up to 40s @16:34:58.03 Intake @15.4psia, vacuum from 8.9 to 12.9psi @16:34:58.04 SS.reconfigure SSinhale.with maxSpeed: 53 @16:34:58.08 SS.seek 25ml @16:35:07.47 Vacuum too high -- waiting up to 40s @16:35:12.33 Intake @15.4psia, vacuum from 8.9 to 12.9psi @16:35:12.34 SS.reconfigure SSinhale.with maxSpeed: 48 @16:35:12.39 SS.seek 25ml @16:35:26.22 Vacuum too high -- waiting up to 40s @16:35:31.58 SS.reconfigure SSinhale.with maxSpeed: 43 @16:35:31.63 SS.seek 25ml @16:35:37.57 Stroke completed -- waiting for pressure to equalize ... @16:35:41.89 Intake.select :close @16:35:42.02 SSV.dial :exhaust @16:35:42.89 Exhaust.select :open @16:35:43.00 SS.reconfigure SSexhale @16:35:43.05 SS.seek 0ml @16:35:52.60 Exhaust.select :close @16:35:52.71 SSV.dial CBV @16:35:52.73
CBV.dial SSV @16:35:53.60 Allowing 130s for 25.0ml of remaining 925.0ml @16:35:53.61 Intake.select :open @16:35:55.77 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:35:55.78 SS.reconfigure SSinhale.with maxSpeed: 43 @16:35:55.83 SS.seek 25ml @16:36:17.50 Vacuum too high -- waiting up to 40s @16:36:22.36 Intake @15.4psia, vacuum from 8.9 to 12.9psi @16:36:22.37 SS.reconfigure SSinhale.with maxSpeed: 39 @16:36:22.41 SS.seek 25ml @16:36:44.10 Stroke completed -- waiting for pressure to equalize ... @16:36:50.18 Intake.select :close @16:36:50.31 SSV.dial :exhaust @16:36:51.18 Exhaust.select :open @16:36:51.29 SS.reconfigure SSexhale @16:36:51.34 SS.seek 0ml @16:37:00.85 Exhaust.select :close @16:37:00.96 SSV.dial CBV @16:37:00.98
CBV.dial SSV @16:37:01.84 Allowing 130s for 25.0ml of remaining 900.0ml @16:37:01.85 Intake.select :open @16:37:04.01 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:37:04.02 SS.reconfigure SSinhale.with maxSpeed: 39 @16:37:04.06 SS.seek 25ml @16:37:49.55 Stroke completed -- waiting for pressure to equalize ... @16:37:57.47 Intake.select :close @16:37:57.60 SSV.dial :exhaust @16:37:58.46 Exhaust.select :open @16:37:58.57 SS.reconfigure SSexhale @16:37:58.62 SS.seek 0ml @16:38:08.13 Exhaust.select :close @16:38:08.24 SSV.dial CBV @16:38:08.26
CBV.dial SSV @16:38:09.13 Allowing 130s for 25.0ml of remaining 875.0ml @16:38:09.14 Intake.select :open @16:38:11.30 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:38:11.31 SS.reconfigure SSinhale.with maxSpeed: 39 @16:38:11.35 SS.seek 25ml @16:38:56.99 Stroke completed -- waiting for pressure to equalize ... @16:39:04.75 Intake.select :close @16:39:04.90 SSV.dial :exhaust @16:39:05.76 Exhaust.select :open @16:39:05.87 SS.reconfigure SSexhale @16:39:05.92 SS.seek 0ml @16:39:15.46 Exhaust.select :close @16:39:15.57 SSV.dial CBV @16:39:15.59
CBV.dial SSV @16:39:16.45 Allowing 130s for 25.0ml of remaining 850.0ml @16:39:16.46 Intake.select :open @16:39:18.62 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:39:18.63 SS.reconfigure SSinhale.with maxSpeed: 39 @16:39:18.68 SS.seek 25ml @16:40:04.29 Stroke completed -- waiting for pressure to equalize ... @16:40:11.59 Intake.select :close @16:40:11.73 SSV.dial :exhaust @16:40:12.59 Exhaust.select :open @16:40:12.70 SS.reconfigure SSexhale @16:40:12.75 SS.seek 0ml @16:40:22.37 Exhaust.select :close @16:40:22.48 SSV.dial CBV @16:40:22.50
CBV.dial SSV @16:40:23.37 Allowing 130s for 25.0ml of remaining 825.0ml @16:40:23.38 Intake.select :open @16:40:25.54 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:40:25.55 SS.reconfigure SSinhale.with maxSpeed: 39 @16:40:25.60 SS.seek 25ml @16:41:11.26 Stroke completed -- waiting for pressure to equalize ... @16:41:18.58 Intake.select :close @16:41:18.72 SSV.dial :exhaust @16:41:19.59 Exhaust.select :open @16:41:19.70 SS.reconfigure SSexhale @16:41:19.75 SS.seek 0ml @16:41:29.40 Exhaust.select :close @16:41:29.51 SSV.dial CBV @16:41:29.53
CBV.dial SSV @16:41:30.38 Allowing 130s for 25.0ml of remaining 800.0ml @16:41:30.39 Intake.select :open @16:41:32.55 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:41:32.56 SS.reconfigure SSinhale.with maxSpeed: 39 @16:41:32.60 SS.seek 25ml @16:42:18.30 Stroke completed -- waiting for pressure to equalize ... @16:42:26.09 Intake.select :close @16:42:26.23 SSV.dial :exhaust @16:42:27.08 Exhaust.select :open @16:42:27.19 SS.reconfigure SSexhale @16:42:27.24 SS.seek 0ml @16:42:36.91 Exhaust.select :close @16:42:37.02 SSV.dial CBV @16:42:37.04
CBV.dial SSV @16:42:37.91 Allowing 130s for 25.0ml of remaining 775.0ml @16:42:37.92 Intake.select :open @16:42:40.08 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:42:40.09 SS.reconfigure SSinhale.with maxSpeed: 39 @16:42:40.13 SS.seek 25ml @16:43:25.58 Stroke completed -- waiting for pressure to equalize ... @16:43:32.89 Intake.select :close @16:43:33.03 SSV.dial :exhaust @16:43:33.90 Exhaust.select :open @16:43:34.01 SS.reconfigure SSexhale @16:43:34.06 SS.seek 0ml @16:43:43.32 Exhaust.select :close @16:43:43.43 SSV.dial CBV @16:43:43.45
CBV.dial SSV @16:43:44.32 Allowing 130s for 25.0ml of remaining 750.0ml @16:43:44.33 Intake.select :open @16:43:46.49 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:43:46.50 SS.reconfigure SSinhale.with maxSpeed: 39 @16:43:46.54 SS.seek 25ml @16:44:32.77 Stroke completed -- waiting for pressure to equalize ... @16:44:40.71 Intake.select :close @16:44:40.84 SSV.dial :exhaust @16:44:41.69 Exhaust.select :open @16:44:41.80 SS.reconfigure SSexhale @16:44:41.85 SS.seek 0ml @16:44:51.37 Exhaust.select :close @16:44:51.48 SSV.dial CBV @16:44:51.50
CBV.dial SSV @16:44:52.35 Allowing 130s for 25.0ml of remaining 725.0ml @16:44:52.36 Intake.select :open @16:44:54.52 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:44:54.53 SS.reconfigure SSinhale.with maxSpeed: 39 @16:44:54.57 SS.seek 25ml @16:45:40.37 Stroke completed -- waiting for pressure to equalize ... @16:45:47.72 Intake.select :close @16:45:47.86 SSV.dial :exhaust @16:45:49.15 Exhaust.select :open @16:45:49.26 SS.reconfigure SSexhale @16:45:49.31 SS.seek 0ml @16:45:58.80 Exhaust.select :close @16:45:58.91 SSV.dial CBV @16:45:58.93
CBV.dial SSV @16:45:59.80 Allowing 130s for 25.0ml of remaining 700.0ml @16:45:59.81 Intake.select :open @16:46:01.97 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:46:01.98 SS.reconfigure SSinhale.with maxSpeed: 39 @16:46:02.03 SS.seek 25ml @16:46:47.76 Stroke completed -- waiting for pressure to equalize ... @16:46:55.69 Intake.select :close @16:46:55.83 SSV.dial :exhaust @16:46:56.68 Exhaust.select :open @16:46:56.79 SS.reconfigure SSexhale @16:46:56.84 SS.seek 0ml @16:47:06.35 Exhaust.select :close @16:47:06.46 SSV.dial CBV @16:47:06.48
CBV.dial SSV @16:47:07.33 Allowing 130s for 25.0ml of remaining 675.0ml @16:47:07.34 Intake.select :open @16:47:09.50 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:47:09.51 SS.reconfigure SSinhale.with maxSpeed: 39 @16:47:09.55 SS.seek 25ml @16:47:55.30 Stroke completed -- waiting for pressure to equalize ... @16:48:03.20 Intake.select :close @16:48:03.34 SSV.dial :exhaust @16:48:04.21 Exhaust.select :open @16:48:04.32 SS.reconfigure SSexhale @16:48:04.37 SS.seek 0ml @16:48:13.91 Exhaust.select :close @16:48:14.02 SSV.dial CBV @16:48:14.04
CBV.dial SSV @16:48:14.91 Allowing 130s for 25.0ml of remaining 650.0ml @16:48:14.92 Intake.select :open @16:48:17.08 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:48:17.09 SS.reconfigure SSinhale.with maxSpeed: 39 @16:48:17.13 SS.seek 25ml @16:49:02.66 Stroke completed -- waiting for pressure to equalize ... @16:49:10.61 Intake.select :close @16:49:11.10 SSV.dial :exhaust @16:49:12.02 Exhaust.select :open @16:49:12.13 SS.reconfigure SSexhale @16:49:12.18 SS.seek 0ml @16:49:21.66 Exhaust.select :close @16:49:21.77 SSV.dial CBV @16:49:21.81
CBV.dial SSV @16:49:22.63 Allowing 130s for 25.0ml of remaining 625.0ml @16:49:22.64 Intake.select :open @16:49:24.80 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:49:24.81 SS.reconfigure SSinhale.with maxSpeed: 39 @16:49:24.85 SS.seek 25ml @16:50:10.54 Stroke completed -- waiting for pressure to equalize ... @16:50:18.48 Intake.select :close @16:50:18.62 SSV.dial :exhaust @16:50:19.49 Exhaust.select :open @16:50:19.60 SS.reconfigure SSexhale @16:50:19.65 SS.seek 0ml @16:50:29.21 Exhaust.select :close @16:50:29.32 SSV.dial CBV @16:50:29.34
CBV.dial SSV @16:50:30.21 Allowing 130s for 25.0ml of remaining 600.0ml @16:50:30.22 Intake.select :open @16:50:32.38 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:50:32.39 SS.reconfigure SSinhale.with maxSpeed: 39 @16:50:32.43 SS.seek 25ml @16:51:18.14 Stroke completed -- waiting for pressure to equalize ... @16:51:26.03 Intake.select :close @16:51:26.16 SSV.dial :exhaust @16:51:27.02 Exhaust.select :open @16:51:27.13 SS.reconfigure SSexhale @16:51:27.18 SS.seek 0ml @16:51:36.71 Exhaust.select :close @16:51:36.82 SSV.dial CBV @16:51:36.84
CBV.dial SSV @16:51:37.69 Allowing 130s for 25.0ml of remaining 575.0ml @16:51:37.70 Intake.select :open @16:51:39.86 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:51:39.87 SS.reconfigure SSinhale.with maxSpeed: 39 @16:51:39.91 SS.seek 25ml @16:52:25.60 Stroke completed -- waiting for pressure to equalize ... @16:52:32.95 Intake.select :close @16:52:33.08 SSV.dial :exhaust @16:52:34.01 Exhaust.select :open @16:52:34.12 SS.reconfigure SSexhale @16:52:34.17 SS.seek 0ml @16:52:43.41 Exhaust.select :close @16:52:43.52 SSV.dial CBV @16:52:43.54
CBV.dial SSV @16:52:44.41 Allowing 130s for 25.0ml of remaining 550.0ml @16:52:44.42 Intake.select :open @16:52:46.58 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:52:46.59 SS.reconfigure SSinhale.with maxSpeed: 39 @16:52:46.64 SS.seek 25ml @16:53:32.80 Stroke completed -- waiting for pressure to equalize ... @16:53:40.75 Intake.select :close @16:53:40.88 SSV.dial :exhaust @16:53:41.74 Exhaust.select :open @16:53:41.85 SS.reconfigure SSexhale @16:53:41.90 SS.seek 0ml @16:53:51.10 Exhaust.select :close @16:53:51.21 SSV.dial CBV @16:53:51.23
CBV.dial SSV @16:53:52.10 Allowing 130s for 25.0ml of remaining 525.0ml @16:53:52.11 Intake.select :open @16:53:54.27 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:53:54.28 SS.reconfigure SSinhale.with maxSpeed: 39 @16:53:54.32 SS.seek 25ml @16:54:40.83 Stroke completed -- waiting for pressure to equalize ... @16:54:48.11 Intake.select :close @16:54:48.25 SSV.dial :exhaust @16:54:49.10 Exhaust.select :open @16:54:49.21 SS.reconfigure SSexhale @16:54:49.26 SS.seek 0ml @16:54:58.47 Exhaust.select :close @16:54:58.58 SSV.dial CBV @16:54:58.60
CBV.dial SSV @16:54:59.46 Allowing 130s for 25.0ml of remaining 500.0ml @16:54:59.47 Intake.select :open @16:55:01.63 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:55:01.64 SS.reconfigure SSinhale.with maxSpeed: 39 @16:55:01.68 SS.seek 25ml @16:55:47.85 Stroke completed -- waiting for pressure to equalize ... @16:55:55.77 Intake.select :close @16:55:55.91 SSV.dial :exhaust @16:55:56.75 Exhaust.select :open @16:55:56.86 SS.reconfigure SSexhale @16:55:57.33 SS.seek 0ml @16:56:06.55 Exhaust.select :close @16:56:06.66 SSV.dial CBV @16:56:06.68
CBV.dial SSV @16:56:07.55 Allowing 130s for 25.0ml of remaining 475.0ml @16:56:07.56 Intake.select :open @16:56:09.72 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:56:09.73 SS.reconfigure SSinhale.with maxSpeed: 39 @16:56:09.78 SS.seek 25ml @16:56:55.97 Stroke completed -- waiting for pressure to equalize ... @16:57:03.91 Intake.select :close @16:57:04.05 SSV.dial :exhaust @16:57:04.91 Exhaust.select :open @16:57:05.02 SS.reconfigure SSexhale @16:57:05.07 SS.seek 0ml @16:57:14.85 Exhaust.select :close @16:57:14.96 SSV.dial CBV @16:57:14.98
CBV.dial SSV @16:57:15.83 Allowing 130s for 25.0ml of remaining 450.0ml @16:57:15.84 Intake.select :open @16:57:18.00 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:57:18.01 SS.reconfigure SSinhale.with maxSpeed: 39 @16:57:18.05 SS.seek 25ml @16:58:03.88 Stroke completed -- waiting for pressure to equalize ... @16:58:11.19 Intake.select :close @16:58:11.32 SSV.dial :exhaust @16:58:12.17 Exhaust.select :open @16:58:12.28 SS.reconfigure SSexhale @16:58:12.33 SS.seek 0ml @16:58:21.91 Exhaust.select :close @16:58:22.02 SSV.dial CBV @16:58:22.04
CBV.dial SSV @16:58:22.91 Allowing 130s for 25.0ml of remaining 425.0ml @16:58:22.92 Intake.select :open @16:58:25.08 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:58:25.09 SS.reconfigure SSinhale.with maxSpeed: 39 @16:58:25.13 SS.seek 25ml @16:59:10.85 Stroke completed -- waiting for pressure to equalize ... @16:59:18.58 Intake.select :close @16:59:18.73 SSV.dial :exhaust @16:59:19.58 Exhaust.select :open @16:59:19.69 SS.reconfigure SSexhale @16:59:19.74 SS.seek 0ml @16:59:29.39 Exhaust.select :close @16:59:29.50 SSV.dial CBV @16:59:29.52
CBV.dial SSV @16:59:30.38 Allowing 130s for 25.0ml of remaining 400.0ml @16:59:30.39 Intake.select :open @16:59:32.55 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:59:32.56 SS.reconfigure SSinhale.with maxSpeed: 39 @16:59:32.61 SS.seek 25ml @17:00:18.11 Stroke completed -- waiting for pressure to equalize ... @17:00:25.43 Intake.select :close @17:00:25.57 SSV.dial :exhaust @17:00:26.44 Exhaust.select :open @17:00:26.55 SS.reconfigure SSexhale @17:00:26.60 SS.seek 0ml @17:00:35.78 Exhaust.select :close @17:00:35.89 SSV.dial CBV @17:00:35.91
CBV.dial SSV @17:00:36.78 Allowing 130s for 25.0ml of remaining 375.0ml @17:00:36.79 Intake.select :open @17:00:38.95 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:00:38.96 SS.reconfigure SSinhale.with maxSpeed: 39 @17:00:39.00 SS.seek 25ml @17:01:25.22 Stroke completed -- waiting for pressure to equalize ... @17:01:33.14 Intake.select :close @17:01:33.27 SSV.dial :exhaust @17:01:34.13 Exhaust.select :open @17:01:34.24 SS.reconfigure SSexhale @17:01:34.29 SS.seek 0ml @17:01:43.86 Exhaust.select :close @17:01:43.97 SSV.dial CBV @17:01:43.99
CBV.dial SSV @17:01:44.84 Allowing 130s for 25.0ml of remaining 350.0ml @17:01:44.85 Intake.select :open @17:01:47.01 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:01:47.02 SS.reconfigure SSinhale.with maxSpeed: 39 @17:01:47.06 SS.seek 25ml @17:02:32.63 Stroke completed -- waiting for pressure to equalize ... @17:02:40.40 Intake.select :close @17:02:40.55 SSV.dial :exhaust @17:02:41.39 Exhaust.select :open @17:02:41.50 SS.reconfigure SSexhale @17:02:41.55 SS.seek 0ml @17:02:51.19 Exhaust.select :close @17:02:51.30 SSV.dial CBV @17:02:51.32
CBV.dial SSV @17:02:52.19 Allowing 130s for 25.0ml of remaining 325.0ml @17:02:52.20 Intake.select :open @17:02:54.36 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:02:54.37 SS.reconfigure SSinhale.with maxSpeed: 39 @17:02:54.42 SS.seek 25ml @17:03:40.05 Stroke completed -- waiting for pressure to equalize ... @17:03:47.94 Intake.select :close @17:03:48.07 SSV.dial :exhaust @17:03:48.94 Exhaust.select :open @17:03:49.05 SS.reconfigure SSexhale @17:03:49.10 SS.seek 0ml @17:03:58.64 Exhaust.select :close @17:03:58.75 SSV.dial CBV @17:03:58.77
CBV.dial SSV @17:03:59.63 Allowing 130s for 25.0ml of remaining 300.0ml @17:03:59.64 Intake.select :open @17:04:01.80 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:04:01.81 SS.reconfigure SSinhale.with maxSpeed: 39 @17:04:01.85 SS.seek 25ml @17:04:47.47 Stroke completed -- waiting for pressure to equalize ... @17:04:55.41 Intake.select :close @17:04:55.55 SSV.dial :exhaust @17:04:56.39 Exhaust.select :open @17:04:56.50 SS.reconfigure SSexhale @17:04:56.55 SS.seek 0ml @17:05:05.77 Exhaust.select :close @17:05:05.88 SSV.dial CBV @17:05:05.90
CBV.dial SSV @17:05:06.77 Allowing 130s for 25.0ml of remaining 275.0ml @17:05:06.78 Intake.select :open @17:05:08.94 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:05:08.95 SS.reconfigure SSinhale.with maxSpeed: 39 @17:05:08.99 SS.seek 25ml @17:05:55.22 Stroke completed -- waiting for pressure to equalize ... @17:06:03.00 Intake.select :close @17:06:03.14 SSV.dial :exhaust @17:06:04.00 Exhaust.select :open @17:06:04.11 SS.reconfigure SSexhale @17:06:04.16 SS.seek 0ml @17:06:13.87 Exhaust.select :close @17:06:13.98 SSV.dial CBV @17:06:14.00
CBV.dial SSV @17:06:14.86 Allowing 130s for 25.0ml of remaining 250.0ml @17:06:14.87 Intake.select :open @17:06:17.03 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:06:17.04 SS.reconfigure SSinhale.with maxSpeed: 39 @17:06:17.08 SS.seek 25ml @17:07:02.50 Stroke completed -- waiting for pressure to equalize ... @17:07:09.79 Intake.select :close @17:07:09.93 SSV.dial :exhaust @17:07:10.78 Exhaust.select :open @17:07:10.89 SS.reconfigure SSexhale @17:07:10.94 SS.seek 0ml @17:07:20.48 Exhaust.select :close @17:07:20.59 SSV.dial CBV @17:07:20.61
CBV.dial SSV @17:07:21.48 Allowing 130s for 25.0ml of remaining 225.0ml @17:07:21.49 Intake.select :open @17:07:23.65 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:07:23.66 SS.reconfigure SSinhale.with maxSpeed: 39 @17:07:23.71 SS.seek 25ml @17:08:09.56 Stroke completed -- waiting for pressure to equalize ... @17:08:16.87 Intake.select :close @17:08:17.00 SSV.dial :exhaust @17:08:17.86 Exhaust.select :open @17:08:17.97 SS.reconfigure SSexhale @17:08:18.02 SS.seek 0ml @17:08:27.22 Exhaust.select :close @17:08:27.33 SSV.dial CBV @17:08:27.35
CBV.dial SSV @17:08:28.20 Allowing 130s for 25.0ml of remaining 200.0ml @17:08:28.21 Intake.select :open @17:08:30.79 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:08:30.80 SS.reconfigure SSinhale.with maxSpeed: 39 @17:08:30.85 SS.seek 25ml @17:09:17.00 Stroke completed -- waiting for pressure to equalize ... @17:09:24.94 Intake.select :close @17:09:25.07 SSV.dial :exhaust @17:09:25.92 Exhaust.select :open @17:09:26.03 SS.reconfigure SSexhale @17:09:26.08 SS.seek 0ml @17:09:35.58 Exhaust.select :close @17:09:35.69 SSV.dial CBV @17:09:35.71
CBV.dial SSV @17:09:36.58 Allowing 130s for 25.0ml of remaining 175.0ml @17:09:36.59 Intake.select :open @17:09:38.75 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:09:38.76 SS.reconfigure SSinhale.with maxSpeed: 39 @17:09:38.81 SS.seek 25ml @17:10:24.51 Stroke completed -- waiting for pressure to equalize ... @17:10:32.44 Intake.select :close @17:10:32.57 SSV.dial :exhaust @17:10:33.44 Exhaust.select :open @17:10:33.55 SS.reconfigure SSexhale @17:10:33.60 SS.seek 0ml @17:10:43.19 Exhaust.select :close @17:10:43.30 SSV.dial CBV @17:10:43.32
CBV.dial SSV @17:10:44.17 Allowing 130s for 25.0ml of remaining 150.0ml @17:10:44.18 Intake.select :open @17:10:46.34 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:10:46.35 SS.reconfigure SSinhale.with maxSpeed: 39 @17:10:46.39 SS.seek 25ml @17:11:32.08 Stroke completed -- waiting for pressure to equalize ... @17:11:40.41 Intake.select :close @17:11:40.56 SSV.dial :exhaust @17:11:41.40 Exhaust.select :open @17:11:41.51 SS.reconfigure SSexhale @17:11:41.56 SS.seek 0ml @17:11:51.04 Exhaust.select :close @17:11:51.15 SSV.dial CBV @17:11:51.17
CBV.dial SSV @17:11:52.04 Allowing 130s for 25.0ml of remaining 125.0ml @17:11:52.05 Intake.select :open @17:11:54.21 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:11:54.22 SS.reconfigure SSinhale.with maxSpeed: 39 @17:11:54.26 SS.seek 25ml @17:12:39.98 Stroke completed -- waiting for pressure to equalize ... @17:12:47.90 Intake.select :close @17:12:48.04 SSV.dial :exhaust @17:12:48.89 Exhaust.select :open @17:12:49.00 SS.reconfigure SSexhale @17:12:49.05 SS.seek 0ml @17:12:58.70 Exhaust.select :close @17:12:58.81 SSV.dial CBV @17:12:58.83
CBV.dial SSV @17:12:59.69 Allowing 130s for 25.0ml of remaining 100.0ml @17:12:59.70 Intake.select :open @17:13:01.86 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:13:01.87 SS.reconfigure SSinhale.with maxSpeed: 39 @17:13:01.91 SS.seek 25ml @17:13:47.31 Stroke completed -- waiting for pressure to equalize ... @17:13:54.65 Intake.select :close @17:13:54.79 SSV.dial :exhaust @17:13:55.64 Exhaust.select :open @17:13:55.75 SS.reconfigure SSexhale @17:13:55.80 SS.seek 0ml @17:14:05.06 Exhaust.select :close @17:14:05.17 SSV.dial CBV @17:14:05.19
CBV.dial SSV @17:14:06.06 Allowing 130s for 25.0ml of remaining 75.0ml @17:14:06.07 Intake.select :open @17:14:08.23 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:14:08.24 SS.reconfigure SSinhale.with maxSpeed: 39 @17:14:08.28 SS.seek 25ml @17:14:54.50 Stroke completed -- waiting for pressure to equalize ... @17:15:02.25 Intake.select :close @17:15:02.39 SSV.dial :exhaust @17:15:03.25 Exhaust.select :open @17:15:03.36 SS.reconfigure SSexhale @17:15:03.41 SS.seek 0ml @17:15:13.12 Exhaust.select :close @17:15:13.23 SSV.dial CBV @17:15:13.25
CBV.dial SSV @17:15:14.11 Allowing 130s for 25.0ml of remaining 50.0ml @17:15:14.12 Intake.select :open @17:15:16.28 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:15:16.29 SS.reconfigure SSinhale.with maxSpeed: 39 @17:15:16.34 SS.seek 25ml @17:16:01.81 Stroke completed -- waiting for pressure to equalize ... @17:16:09.11 Intake.select :close @17:16:09.25 SSV.dial :exhaust @17:16:10.09 Exhaust.select :open @17:16:10.20 SS.reconfigure SSexhale @17:16:10.25 SS.seek 0ml @17:16:19.81 Exhaust.select :close @17:16:19.92 SSV.dial CBV @17:16:19.94
CBV.dial SSV @17:16:20.81 Allowing 130s for 25.0ml of remaining 25.0ml @17:16:20.82 Intake.select :open @17:16:22.98 Intake @15.8psia, vacuum from 9.3 to 13.3psi @17:16:22.99 SS.reconfigure SSinhale.with maxSpeed: 39 @17:16:23.03 SS.seek 24.97ml @17:17:08.68 Stroke completed -- waiting for pressure to equalize ... @17:17:16.58 Intake.select :close @17:17:16.72 SSV.dial :exhaust @17:17:17.59 Exhaust.select :open @17:17:17.70 SS.reconfigure SSexhale @17:17:17.75 SS.seek 0ml @17:17:27.40 Exhaust.select :close @17:17:27.51 Sampled 1000.0ml @17:17:27.53 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) Intake.select :close @17:17:27.59 CSV.dial :waste @17:17:27.64 CS.seek 0ml @17:17:27.73 SSV.dial CBV,avoiding: :exhaust @17:17:27.75
CBV.dial SSV @17:17:27.81 CSV.dial :air @17:17:28.66 CS.seek 10ml @17:17:29.87
CTV.dial :air @17:17:30.51 Gate.power :sampler,:OFF @17:17:31.15
delay 15 seconds @17:17:46.17 SSV.dial :spit,avoiding: :exhaust @17:17:47.65 CTV.dial CSV,avoiding: :intake @17:17:47.69
SS.seek 0ml @17:17:47.80 SSV.dial CBV,avoiding: :exhaust @17:17:55.74 CBV.dial between SSV and waste2 @17:17:56.16 CTV.dial CSV,avoiding: :air @17:17:56.19
CSV.dial CTV @17:17:56.69 CS.reconfigure CSpressurize @17:17:56.73 CS.seek 0ml @17:18:22.93 CSV.dial :air @17:18:23.45 CS.reconfigure CSfast @17:18:23.49 CS.seek 10ml @17:18:50.11 CSV.dial between air and CTV @17:18:50.58 CS.seek 2.532ml @17:19:10.90 CTV.dial CSV,avoiding: :air @17:19:10.92
CSV.dial CTV @17:19:11.28 CS.reconfigure CSpressurize @17:19:11.32 CS.seek 0ml @17:19:15.36 BubblePoint: Intake @70.5psia, Outlet @15.2psia @17:19:15.37 CBV.dial SSV @17:19:15.74 SS.reconfigure SSconfig @17:19:15.79 SS.seek 10ml @17:19:21.65 delay 15 seconds @17:19:36.77 Evacuated: Intake @38.8psia, Outlet @12.5psia @17:19:36.78 SSV.dial :spit,avoiding: :exhaust @17:19:38.26 SS.reconfigure SSexhale @17:19:38.31 SS.seek 0ml @17:19:43.60 SS.reconfigure SSfast @17:19:43.65 SS.seek 5ml @17:19:46.48 SSV.dial CBV,avoiding: :exhaust @17:19:46.51
CBV.dial SSV @17:19:48.00 CTV.dial :air,avoiding: :intake @17:19:49.52 CSV.dial CTV @17:19:49.56
SS.seek 10ml @17:19:50.55 CSV.dial :air @17:19:51.07 CS.reconfigure CSfast @17:19:51.12 CS.seek 0ml @17:19:52.07
SSV.dial :spit,avoiding: :exhaust @17:19:53.58 SS.reconfigure SSexhale @17:19:53.62 SS.seek 0ml @17:19:55.22 CS.coast @17:19:58.97
SS.reconfigure SSfast @17:19:59.02 SS.seek 5ml @17:20:01.84 SSV.dial CBV,avoiding: :exhaust @17:20:01.87
CBV.dial SSV @17:20:04.35 SSV.dial :spit,avoiding: :exhaust @17:20:05.84 SS.reconfigure SSconfig @17:20:05.89 SS.seek 0ml @17:20:08.98 SS.coast @17:20:09.01 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @17:20:09.02 Intake.select :close @17:20:09.09 CSV.dial :waste @17:20:09.20 SSV.dial CBV,avoiding: :exhaust @17:20:09.22
CBV.dial SSV @17:20:09.99 CS.seek 0ml @17:20:10.05 CSV.dial :air @17:20:10.73
CTV.dial :air @17:20:10.74 delay 15 seconds @17:20:10.91 CS.seek 10ml @17:20:25.76
SSV.dial :spit,avoiding: :exhaust @17:20:27.65 CTV.dial CSV,avoiding: :intake @17:20:27.69
SS.reconfigure SSexhale @17:20:27.78 SS.seek 0ml @17:20:27.84 SSV.dial CBV,avoiding: :exhaust @17:20:37.65 CBV.dial between SSV and waste2 @17:20:38.08 CSV.dial between air and CTV @17:20:38.42 CS.seek 8.079ml @17:20:44.34 CTV.dial CSV,avoiding: :air @17:20:44.37
CSV.dial CTV @17:20:44.71 CS.reconfigure CSpressurize @17:20:44.75 CS.seek 0ml @17:21:05.96 CSV.dial :air @17:21:06.49 CS.reconfigure CSfast @17:21:06.53 CS.seek 10ml @17:21:32.90 CSV.dial between air and CTV @17:21:33.25 CS.seek 3.046ml @17:21:51.90 CTV.dial CSV,avoiding: :air @17:21:51.92
CSV.dial CTV @17:21:52.25 CS.reconfigure CSpressurize @17:21:52.29 CS.seek 0ml @17:21:59.97 BubblePoint: Intake @70.2psia, Outlet @14.7psia CBV.dial SSV @17:22:00.32 SS.reconfigure SSconfig @17:22:00.37 SS.seek 10ml @17:22:05.82 delay 15 seconds @17:22:20.94 Evacuated: Intake @41.4psia, Outlet @16.0psia @17:22:20.95 SSV.dial :spit,avoiding: :exhaust @17:22:22.44 SS.reconfigure SSexhale @17:22:22.48 SS.seek 0ml @17:22:28.06 SS.reconfigure SSfast @17:22:28.11 SS.seek 5ml @17:22:30.71 SSV.dial CBV,avoiding: :exhaust @17:22:30.74
CBV.dial SSV @17:22:32.23 CTV.dial :air,avoiding: :intake @17:22:33.76 CSV.dial CTV @17:22:33.80
SS.seek 10ml @17:22:34.79 CSV.dial :air @17:22:35.31 CS.reconfigure CSfast @17:22:35.36 CS.seek 0ml @17:22:36.53
SSV.dial :spit,avoiding: :exhaust @17:22:37.18 CS.coast @17:22:38.02
SS.reconfigure SSexhale @17:22:38.06 SS.seek 0ml @17:22:43.15 SS.reconfigure SSfast @17:22:43.20 SS.seek 5ml @17:22:46.20 SSV.dial CBV,avoiding: :exhaust @17:22:46.23
CBV.dial SSV @17:22:48.75 SSV.dial :spit,avoiding: :exhaust @17:22:50.24 SS.reconfigure SSconfig @17:22:50.29 SS.seek 0ml @17:22:53.54 SS.coast @17:22:53.57 Lysing: Adding 1.5ml lysis @17:22:53.58 CBV.dial between CSV and waste1,avoiding: :DBV @17:22:55.29 CTV.dial CSV @17:22:56.79 CSV.dial CSR @17:22:57.68 Pulling 1ml of CSR.lysis @17:22:57.69 CSR.select :lysis @17:22:58.21 CS.reconfigure CSconfig @17:22:58.26 CS.seek 1ml @17:23:01.81 delay 5 seconds @17:23:06.83 CSR.select :air @17:23:06.99 CS.seek 6ml @17:23:20.71 delay 2 seconds @17:23:22.73 CSV.dial :waste1 @17:23:24.21 CS.reconfigure CSfast @17:23:24.26 CS.seek :empty @17:23:40.40 CSV.dial CSR @17:23:41.94 Pulling 1.5ml of CSR.lysis @17:23:41.95 CSR.select :lysis @17:23:42.06 CS.reconfigure CSconfig @17:23:42.11 CS.seek 1.5ml @17:23:46.90 delay 5 seconds @17:23:51.92 CSR.select :air @17:23:52.08 CS.seek 4.5ml @17:24:00.68 delay 2 seconds @17:24:02.70 CSV.dial CTV @17:24:03.22 CS.seek 1.275ml @17:24:12.51 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @17:24:12.52 CH.seek 85C,8:00 @17:29:43.85 CH.hold 5:00,85C @17:34:44.00 Collection Puck Heater at 83.9C after lysing @17:34:44.02 CS.seek 0ml @17:34:48.70 CTV.dial between spare and air,avoiding: :intake @17:34:49.97 CSV.dial :air @17:34:50.46 CS.seek 5ml @17:35:04.31 CSV.dial CTV @17:35:04.81 delay 2 seconds @17:35:06.83 CSV.dial :air @17:35:07.35 delay 2 seconds @17:35:09.37 CSV.dial CTV @17:35:09.89 delay 2 seconds @17:35:11.91 CSV.dial :air @17:35:12.42 delay 2 seconds @17:35:14.45 CS.seek 0ml @17:35:28.22 Recovering Lysate into Collection syringe @17:35:28.23 CSV.dial CBV @17:35:29.52 CBV.dial CSV @17:35:29.92 CS.seek 5ml @17:35:43.72 delay 5 seconds @17:35:48.74 CTV.dial :air @17:35:49.31 CS.seek 7ml @17:35:55.44 delay 5 seconds @17:36:00.46 CSV.dial :air2 @17:36:00.98 CS.seek 10ml @17:36:09.66 CSV.dial between air and CTV,avoiding: CBV @17:36:11.08 Lysing Complete @17:36:11.15 Storing sh1 puck in Collection Clamp to tube 1 @17:36:11.31 SC.reconfigure SCtight @17:36:11.35 SC.seek 1 @17:36:13.51 SC.reconfigure SCloose @17:36:13.70 Elbow.reconfigure TightElbow @17:36:13.74 Elbow.seek 1 @17:36:15.65 Forearm.reconfigure TightForearm @17:36:15.70 Forearm.seek 1 + 700 counts @17:36:16.81 SE.reconfigure SEpick @17:36:16.88 SE.seek beamBreak @17:36:19.82 SE.configure SEconfig @17:36:19.87 SE.seek :place @17:36:20.47 Forearm.reconfigure LooseForearm @17:36:20.52 Forearm.seek :retract @17:36:21.45 Elbow.seek CC @17:36:23.10 Elbow.reconfigure LooseElbow @17:36:23.18 Forearm.seek CC @17:36:24.26 Hand.close @17:36:25.06 CC.seek :open @17:36:29.54 Forearm.seek :retract @17:36:30.60 Elbow.reconfigure TightElbow @17:36:30.64 Elbow.seek 1 @17:36:32.54 Elbow.reconfigure LimpElbow @17:36:32.58 Forearm.seek 1 @17:36:33.62 Elbow.hold @17:36:33.68 Elbow.reconfigure TightElbow @17:36:33.72 Hand.open @17:36:35.05 Forearm.force -20 @17:36:35.09 Forearm.seek 1 + 346 counts @17:36:35.40 Forearm.reconfigure TightForearm @17:36:35.44 Forearm.seek 1 + 700 counts @17:36:35.72 SE.seek :down @17:36:36.85
Forearm.reconfigure LooseForearm @17:36:36.89 Forearm.seek :retract @17:36:38.55 SE.coast @17:36:38.58
SC.coast @17:36:38.61 Tranferring lysate to PRV @17:36:38.62 CSV.dial :air @17:36:38.98 CS.seek 10ml @17:36:39.04 CSV.dial between air and CTV @17:36:39.39 AIV.dial :bypass @17:36:39.47 Beginning samplePassC @17:36:39.49 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:36:39.55 Hand.open @17:36:39.93 Elbow.seek FlushPuck::Garage @17:36:42.11 Elbow.reconfigure LooseElbow @17:36:42.20 Forearm.seek :garage @17:36:43.21 Hand.close @17:36:44.04 Forearm.seek :retract @17:36:45.07 Elbow.reconfigure TightElbow @17:36:45.11 Elbow.seek CC @17:36:48.01 Elbow.reconfigure LooseElbow @17:36:48.09 Forearm.reconfigure TightForearm @17:36:48.13 Forearm.seek CC @17:36:49.46 CC.seek :closed @17:36:53.80 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.38 @17:36:53.86 Hand.open @17:36:54.62 Forearm.reconfigure LooseForearm @17:36:54.66 Forearm.seek :retract @17:36:55.68 AWV.select :air @17:36:55.69 AIV.dial :bypass @17:36:55.70 PSV.dial PSR @17:36:57.23 Pulling 1.5ml of PSR.flush @17:36:57.24 PSR.select :flush @17:36:57.37 PS.seek 1.5ml @17:37:13.63 delay 5 seconds @17:37:18.65 PSV.dial :waste1 @17:37:19.17 PS.seek 0ml @17:37:35.29 PSV.dial PSR @17:37:35.79 PSR.select :air @17:37:35.91 PS.seek 2.5ml @17:38:02.22 PRV.dial CBV @17:38:02.26
CBV.dial PRV @17:38:03.16 PSV.dial :mixloop @17:38:04.30 CTV.dial :air,avoiding: :intake @17:38:04.75 PTV.dial :PRVmixing @17:38:04.84 PS.seek 0ml @17:38:31.16 PSV.dial :air @17:38:31.67 PS.seek 2.5ml @17:38:58.07 PSV.dial :mixloop @17:38:58.58 PS.seek 0ml @17:39:24.72 PSV.dial :air @17:39:25.23 PS.seek 2.5ml @17:39:51.35 PSV.dial :mixloop @17:39:51.86 PS.seek 0ml @17:40:18.07 Garaging FlushPuck in Collection Clamp @17:40:18.14 Elbow.reconfigure TightElbow @17:40:18.18 Elbow.seek CC @17:40:18.42 Elbow.reconfigure LooseElbow @17:40:18.51 Forearm.seek CC @17:40:19.58 Hand.close @17:40:20.32 CC.seek :open @17:40:24.68 Forearm.seek :retract @17:40:25.75 Elbow.reconfigure TightElbow @17:40:25.79 Elbow.seek :garage @17:40:29.02 Elbow.reconfigure LooseElbow @17:40:29.11 Forearm.reconfigure TightForearm @17:40:29.16 Forearm.seek :garage @17:40:30.33 Hand.open @17:40:31.12 Forearm.reconfigure LooseForearm @17:40:31.16 Forearm.seek :retract @17:40:32.20 Connecting PS to CS to transfer sample @17:40:32.21 CBV.dial AIV @17:40:32.22 AIV.dial :bypass @17:40:32.23 PSV.dial :mixloop @17:40:32.24 PTV.dial :PRVmixing @17:40:32.25 PRV.dial AIV @17:40:32.26 CTV.dial :air,avoiding: :intake @17:40:32.28 Loading lyfil puck from tube 2 into Collection Clamp @17:40:32.49 SC.reconfigure SCtight @17:40:32.53 SC.seek 2 @17:40:34.69 SC.reconfigure SCloose @17:40:34.88 Elbow.reconfigure TightElbow @17:40:34.92 Elbow.seek 2 @17:40:37.13 Forearm.reconfigure TightForearm @17:40:37.17 Forearm.seek 2 + 700 counts @17:40:38.26 SE.reconfigure SEpick @17:40:38.33 SE.seek beamBreak @17:40:41.40 SE.configure SEconfig @17:40:41.54 Elbow.seek 2 @17:40:41.70 Forearm.seek 2 + 300 counts @17:40:41.97 Forearm.reconfigure LooseForearm @17:40:42.01 Hand.close @17:40:42.82 SE.seek :down @17:40:43.02
Forearm.seek :retract @17:40:43.97 Elbow.seek CC @17:40:45.61 Elbow.reconfigure LooseElbow @17:40:45.68 SE.coast @17:40:45.78
Forearm.reconfigure TightForearm @17:40:45.82 Forearm.seek CC @17:40:47.55 CC.seek :closed @17:40:51.81 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.47 @17:40:51.82 Hand.open @17:40:52.63 Forearm.reconfigure LooseForearm @17:40:52.67 Forearm.seek :retract @17:40:53.72 SC.coast @17:40:53.75 CTV.dial CSV,avoiding: :intake @17:40:55.29 CSV.dial CTV @17:40:55.62 CS.seek 5ml @17:41:09.38 delay 1:20 @17:42:29.42 CS.seek 2.5ml @17:42:36.85 delay 40 seconds @17:43:16.88 CS.seek 0ml @17:43:24.29 delay 1:00 @17:44:24.31 PSV.dial :air @17:44:24.83 PS.seek 2.5ml @17:44:51.23 PSV.dial :mixloop @17:44:51.73 delay 3 seconds @17:44:54.75 PSV.dial :air @17:44:55.26 delay 2 seconds @17:44:57.28 PSV.dial :mixloop @17:44:57.79 delay 3 seconds @17:45:00.81 PSV.dial :waste1 @17:45:02.30 PS.seek :empty @17:45:28.41 CBV.dial :waste1 @17:45:28.95 delay 5 seconds @17:45:33.97 CBV.dial AIV @17:45:34.51 delay 10 seconds @17:45:44.54 CBV.dial :waste1 @17:45:45.05 delay 10 seconds @17:45:55.08 CBV.dial AIV @17:45:55.60 delay 10 seconds @17:46:05.63 CTV.dial CSV,avoiding: :intake @17:46:05.64 CBV.dial between AIV and waste1 @17:46:06.02 CSV.dial :air @17:46:06.55 CS.seek 10ml @17:46:33.07 CSV.dial CTV @17:46:33.60 CS.seek 0ml @17:47:00.29 CBV.dial :waste1 @17:47:00.63 delay 10 seconds @17:47:10.66 CTV.dial :air,avoiding: :intake @17:47:12.26 Storing lyfil puck in Collection Clamp to tube 1 @17:47:12.41 SC.reconfigure SCtight @17:47:12.45 SC.seek 1 @17:47:14.54 SC.reconfigure SCloose @17:47:14.73 Elbow.reconfigure TightElbow @17:47:14.78 Elbow.seek 1 @17:47:16.65 Forearm.reconfigure TightForearm @17:47:16.70 Forearm.seek 1 + 700 counts @17:47:17.71 SE.reconfigure SEpick @17:47:17.78 SE.seek beamBreak @17:47:20.88 SE.configure SEconfig @17:47:21.02 Elbow.reconfigure LimpElbow @17:47:21.06 Forearm.reconfigure LooseForearm @17:47:21.10 Forearm.seek 1 @17:47:21.38 Elbow.hold @17:47:21.44 Elbow.reconfigure TightElbow @17:47:21.53 SE.seek 0.07 Pucks below Top Plate @17:47:21.95 Forearm.force -20 @17:47:21.99 Forearm.seek 1 + 349 counts @17:47:22.68 Forearm.reconfigure TightForearm @17:47:22.78 Forearm.seek 1 + 700 counts @17:47:23.00 SE.seek 1.07 Pucks below Top Plate @17:47:23.58 Forearm.reconfigure LooseForearm @17:47:23.62 Forearm.seek :retract @17:47:24.54 Elbow.seek CC @17:47:26.13 Elbow.reconfigure LooseElbow @17:47:26.22 Forearm.seek CC @17:47:27.30 Hand.close @17:47:28.07 CC.seek :open @17:47:32.54 Forearm.seek :retract @17:47:33.57 Elbow.reconfigure TightElbow @17:47:33.62 Elbow.seek 1 @17:47:35.54 Elbow.reconfigure LimpElbow @17:47:35.59 Forearm.seek 1 @17:47:36.63 Elbow.hold @17:47:36.69 Elbow.reconfigure TightElbow @17:47:36.74 Hand.open @17:47:37.57 Forearm.force -20 @17:47:37.60 Forearm.seek 1 + 343 counts @17:47:37.91 Forearm.reconfigure TightForearm @17:47:37.95 Forearm.seek 1 + 700 counts @17:47:38.28 SE.seek :down @17:47:39.40
Forearm.reconfigure LooseForearm @17:47:39.44 Forearm.seek :retract @17:47:41.00 SE.coast @17:47:41.04
SC.coast @17:47:41.07 PSV.dial :air @17:47:42.38 PS.seek 0.499ml @17:47:48.29 delay 3 seconds @17:47:51.31 PSV.dial :mixloop @17:47:51.80 PRV.dial :air @17:47:52.30 delay 3 seconds @17:47:55.32 PRV.dial CBV @17:47:55.84 CBV.dial AIV @17:47:56.35 PRV.dial between CBV and air @17:47:56.70 PRV.dial :air @17:47:57.08 PS.reconfigure PSslow @17:47:57.12 PS.seek 0.599ml @17:47:59.63 PRV.dial :waste2 @17:48:00.46 PS.reconfigure PSconfig @17:48:00.50 PS.seek :empty @17:48:07.45 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:48:07.51 Hand.open @17:48:07.81 Elbow.seek FlushPuck::Garage @17:48:09.86 Elbow.reconfigure LooseElbow @17:48:09.95 Forearm.seek :garage @17:48:10.99 Hand.close @17:48:11.82 Forearm.seek :retract @17:48:12.88 Elbow.reconfigure TightElbow @17:48:12.92 Elbow.seek CC @17:48:15.84 Elbow.reconfigure LooseElbow @17:48:16.38 Forearm.reconfigure TightForearm @17:48:16.42 Forearm.seek CC @17:48:17.72 CC.seek :closed @17:48:22.06 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.44 @17:48:22.13 Hand.open @17:48:22.88 Forearm.reconfigure LooseForearm @17:48:22.93 Forearm.seek :retract @17:48:24.02 Clearing PML for modified lysate @17:48:24.03 CTV.dial :air,avoiding: :intake @17:48:24.04 PRV.dial :waste2 @17:48:24.05 PTV.dial :PRV,avoiding: :puck @17:48:24.88 delay 2 seconds @17:48:26.90 PTV.dial :PRVmixing,avoiding: :puck @17:48:27.73 delay 2 seconds @17:48:29.75 PSV.dial :PML @17:48:29.76 delay 2 seconds @17:48:31.78 PRV.dial :air @17:48:32.60 delay 2 seconds @17:48:34.68 PS.seek 1.499ml @17:48:50.76 delay 5 seconds @17:48:55.79 PS.seek 2.5ml @17:49:06.76 PSV.dial :air @17:49:07.25 PS.seek 0ml @17:49:33.48 mixing lysate with speDil @17:49:33.49 PSV.dial :PML @17:49:33.98 CBV.dial :PRV @17:49:33.99 PRV.dial :air @17:49:34.00 delay 2 seconds @17:49:36.02 PRV.dial :speDil @17:49:36.54 delay 2 seconds @17:49:38.63 PS.reconfigure PSslow1 @17:49:38.67 PS.seek 0.225ml @17:50:02.85 delay 5 seconds @17:50:07.87 PRV.dial CBV @17:50:08.73 delay 2 seconds @17:50:10.81 PS.seek 0.475ml @17:50:36.66 delay 5 seconds @17:50:41.68 PRV.dial :air @17:50:42.20 delay 2 seconds @17:50:44.23 PS.seek 0.965ml @17:51:34.38 delay 10 seconds @17:51:44.40 PRV.dial CBV @17:51:44.90 CTV.dial :air,avoiding: :intake @17:51:44.91 PSV.dial :air @17:51:45.40 PS.reconfigure PSconfig @17:51:45.45 PS.seek 2.5ml @17:52:01.85 delay 3 seconds @17:52:04.87 PSV.dial PTV @17:52:05.70 PTV.dial :PRV,avoiding: :puck @17:52:06.52 PS.seek 0ml @17:52:32.70 PSV.dial :air @17:52:33.53 PS.seek 2.5ml @17:52:59.76 delay 3 seconds @17:53:02.78 PSV.dial PTV @17:53:03.60 PTV.dial :PRV,avoiding: :puck @17:53:03.62 PS.seek 0ml @17:53:29.79 Pushing modified lysate into AIV Injection Loop @17:53:29.80 CBV.dial AIV @17:53:29.81 AIV.dial :bypass @17:53:29.82 PRV.dial CBV @17:53:29.83 PSV.dial :air @17:53:30.68 PS.seek 2.5ml @17:53:56.85 delay 3 seconds @17:53:59.87 PSV.dial :PML @17:54:00.37 PTV.dial :PRVmixing,avoiding: :puck @17:54:01.68 PS.reconfigure PSslow1 @17:54:01.72 PS.seek 1.74ml @17:55:19.04 delay 5 seconds @17:55:24.06 AIV.dial :sample @17:55:24.70 AWV.select :waste @17:55:24.85 PS.reconfigure PSslow2 @17:55:24.90 PS.seek 1.22ml @17:57:12.79 delay 15 seconds @17:57:27.81 AIV.dial :bypass @17:57:28.45 PS.reconfigure PSconfig @17:57:28.49 PS.seek 0ml @17:57:41.73 PSV.dial :air @17:57:42.24 PS.seek 2.5ml @17:58:08.45 PSV.dial :PML @17:58:08.96 PS.seek 0ml @17:58:35.17 AIV.dial :coilToMFB @17:58:35.18 AWV.select :air @17:58:35.33 Extracting Nucleic Acid from AIV @17:58:35.36 SPE.hold 2:46:40,55C @17:58:35.38
Beginning samplePassD @17:58:35.45 ASV.dial :SPEdelivery @17:58:35.53
AWV.select :air @17:58:35.54 AIV.dial :bypass @17:58:35.55 PSV.dial PSR @17:58:35.88 delay 20 seconds @17:58:36.81
Pulling 1.5ml of PSR.killMFB PSR.select :killMFB @17:58:36.94 PS.seek 1.5ml @17:58:53.82 delay 5 seconds @17:58:55.90 primeLysateINT @17:58:55.91 AWV.select :air @17:58:55.92 AIV.dial :coilToMFB @17:58:55.93 ATV.dial AIV @17:58:57.88 AS.reconfigure ASslow1 @17:58:57.92 AS.seek 0.299ml @17:58:58.84
PSV.dial :waste1 @17:58:59.38 PS.seek 0ml @17:59:16.29 PSV.dial PSR @17:59:16.82 PSR.select :air @17:59:16.94 PS.seek 2.5ml @17:59:44.57 PRV.dial CBV @17:59:44.59
CBV.dial PRV @17:59:44.61 PSV.dial :mixloop @17:59:45.79 CTV.dial :air,avoiding: :intake @17:59:45.80 PTV.dial :PRVmixing @17:59:45.82 PS.seek 0ml @17:59:58.52 delay 10 seconds @18:00:08.55 ATV.dial :air2 @18:00:09.46 AS.seek 0.329ml @18:00:13.58
PSV.dial PSR @18:00:14.84 Pulling 1.5ml of PSR.flush PSR.select :flush @18:00:14.97 PS.seek 1.5ml @18:00:15.77 ATV.dial :waste2 @18:00:16.38 AS.reconfigure ASnorm @18:00:16.43 AS.seek :empty @18:00:31.86
delay 5 seconds @18:00:33.44 Lysate primed! @18:00:33.45 AFV.select :holdingCoil @18:00:33.46 ATV.dial :air2 @18:00:34.13 AS.reconfigure ASslow1 @18:00:34.17 AS.seek 0.069ml @18:00:36.88
PSV.dial :waste1 @18:00:37.52 PS.seek 0ml @18:00:48.57 delay 3 seconds @18:00:51.59 ATV.dial :AIV @18:00:52.49 AS.seek 0.469ml @18:00:54.51
PSV.dial PSR @18:00:55.14 PSR.select :air @18:00:55.26 PS.seek 2.5ml @18:01:22.79 PRV.dial CBV @18:01:22.81
CBV.dial PRV @18:01:22.83 PSV.dial :mixloop @18:01:24.03 CTV.dial :air,avoiding: :intake @18:01:24.04 PTV.dial :PRVmixing @18:01:24.06 PS.seek 0ml @18:01:51.26 PSV.dial PSR @18:01:52.51 Pulling 1.5ml of PSR.flush PSR.select :flush @18:01:53.07 PS.seek 1.5ml @18:02:09.70 delay 5 seconds @18:02:13.19 delay 5 seconds @18:02:14.72
PSV.dial :waste1 @18:02:15.24 PS.seek 0ml @18:02:18.26 Solid Phase Extraction Heater at 49.3C @18:02:18.27 ATV.dial ASV @18:02:18.69 ASV.dial :SPEdelivery @18:02:18.76 AS.seek 0.041ml @18:02:32.07
PSV.dial PSR @18:02:32.59 PSR.select :air @18:02:32.71 PS.seek 2.5ml @18:02:59.70 PRV.dial CBV @18:02:59.73
CBV.dial PRV @18:02:59.74 PSV.dial :mixloop @18:03:00.90 CTV.dial :air,avoiding: :intake @18:03:00.91 PTV.dial :PRVmixing @18:03:00.93 PS.seek 0ml @18:03:27.86 PSV.dial PSR @18:03:29.09 Pulling 1.5ml of PSR.flush PSR.select :flush @18:03:29.22 PS.seek 1.5ml @18:03:45.21 delay 10 seconds @18:03:45.54
delay 5 seconds @18:03:50.56 PSV.dial :waste1 @18:03:51.07 PS.seek 0ml @18:03:55.23 rinseLysateLineINT @18:03:55.24 AFV.select :carrierSolution @18:03:55.34 delay 2 seconds @18:03:57.37 AS.reconfigure ASconfig @18:03:57.42 AS.seek 1ml @18:04:07.42
PSV.dial PSR @18:04:07.93 PSR.select :air @18:04:08.05 PS.seek 2.5ml @18:04:34.92 PRV.dial CBV @18:04:34.94
CBV.dial PRV @18:04:34.96 PSV.dial :mixloop @18:04:36.11 CTV.dial :air,avoiding: :intake @18:04:36.12 PTV.dial :PRVmixing @18:04:36.14 PS.reconfigure PSslow @18:04:36.18 PS.seek 1.2ml @18:04:52.79 delay 1 second @18:04:53.81 AFV.select :holdingCoil @18:04:53.91 delay 1 second @18:04:54.93 ATV.dial AIV @18:04:55.49 AIV.dial :coilToMFB @18:04:55.50 AWV.select :waste @18:04:55.61 AS.reconfigure ASslow0 @18:04:55.65 AS.seek 0ml @18:05:02.98
PSV.dial :waste1 @18:05:04.47 PS.reconfigure PSconfig @18:05:04.52 PS.seek 0ml @18:05:17.67 PSV.dial :air @18:05:18.84 PS.seek 2.5ml @18:05:45.39 PSV.dial :mixloop @18:05:45.88 PS.reconfigure PSslow @18:05:45.92 PS.seek 0ml @18:06:36.54 ATV.dial :air2 @18:06:36.95
PSV.dial :air @18:06:37.22 AS.reconfigure ASslow1 @18:06:37.26 AS.seek 0.05ml @18:06:37.46
PS.reconfigure PSconfig @18:06:37.50 PS.seek 2.5ml @18:06:47.69 ATV.dial :AIV @18:06:48.53 AS.seek 0.01ml @18:06:56.85 ATV.dial :waste @18:06:58.55 AS.seek :empty @18:07:00.97 ethanol wash @18:07:00.98 ATV.dial :air2 @18:07:02.28 AS.reconfigure ASslow2 @18:07:02.32 AS.seek 0.009ml @18:07:04.01
PSV.dial :mixloop @18:07:04.52 PS.reconfigure PSslow @18:07:04.56 PS.seek 0ml @18:07:12.69 delay 3 seconds @18:07:15.71 Pulling 0.4ml of Ethanol Wash @18:07:15.72 ATV.dial :ethanol @18:07:16.57 AS.reconfigure ASslow1 @18:07:16.61 AS.seek 0.409ml @18:07:55.73
Cleaning Collection Syringe @18:07:55.74 CSV.dial CSR @18:07:56.74 Pulling 4ml of CSR.flush CSR.select :flush @18:07:56.87 CS.seek 4ml @18:08:08.24 delay 5 seconds @18:08:13.26 CSR.select :air @18:08:13.38 CS.seek 10ml @18:08:29.80 CSV.dial CTV @18:08:29.83
CTV.dial CSV @18:08:31.43 CBV.dial :waste1 @18:08:31.95 CS.seek 0ml @18:08:37.30 delay 3 seconds @18:08:40.32 ATV.dial :air1 @18:08:40.72 delay 3 seconds @18:08:43.79 AS.seek 0.434ml @18:08:49.08 delay 3 seconds @18:08:52.10 ATV.dial :ethanol @18:08:52.71 AS.seek 0.419ml @18:08:56.05 delay 3 seconds @18:08:58.43
CSV.dial CSR @18:08:58.99 Pulling 4ml of CSR.flush CSR.select :flush @18:08:59.07 ATV.dial ASV @18:08:59.13
CS.seek 4ml @18:09:00.44 AS.seek 0.092ml @18:09:10.46
delay 5 seconds @18:09:15.48 CSR.select :air @18:09:15.60 CS.seek 10ml @18:09:31.87 CSV.dial CTV @18:09:31.90
CTV.dial CSV @18:09:32.43 CBV.dial :waste1 @18:09:32.45 CS.seek 0ml @18:09:59.05 Garaging FlushPuck in Collection Clamp @18:09:59.11 Elbow.reconfigure TightElbow @18:09:59.15 Elbow.seek CC @18:09:59.39 Elbow.reconfigure LooseElbow @18:09:59.48 Forearm.seek CC @18:10:00.52 Hand.close @18:10:01.23 CC.seek :open @18:10:05.64 Forearm.seek :retract @18:10:06.49 delay 3 seconds @18:10:06.67
Elbow.reconfigure TightElbow @18:10:06.71 Elbow.seek :garage @18:10:09.51 ATV.dial :waste2 @18:10:09.84
Elbow.reconfigure LooseElbow @18:10:09.93 Forearm.reconfigure TightForearm @18:10:09.97 Forearm.seek :garage @18:10:10.38 AS.reconfigure ASconfig @18:10:10.43 AS.seek :empty @18:10:11.14
Hand.open @18:10:11.91 Forearm.reconfigure LooseForearm @18:10:11.95 Forearm.seek :retract @18:10:13.00 Whole Cell Archive sampling 1500ml @18:10:13.02 Loading wcr puck from tube 2 into Collection Clamp @18:10:13.24 SC.reconfigure SCtight @18:10:13.28 SC.seek 2 @18:10:15.47 SC.reconfigure SCloose @18:10:15.67 Elbow.reconfigure TightElbow @18:10:15.71 Elbow.seek 2 @18:10:16.07 ATV.dial :air2 @18:10:16.48 AS.reconfigure ASslow1 @18:10:16.52 AS.seek 1ml @18:10:17.78 Forearm.reconfigure TightForearm @18:10:17.82 Forearm.seek 2 + 700 counts @18:10:19.43 SE.reconfigure SEpick @18:10:19.50 SE.seek beamBreak @18:10:22.68 SE.configure SEconfig @18:10:22.84 Elbow.seek 2 @18:10:23.03 Forearm.seek 2 + 300 counts @18:10:23.21 Forearm.reconfigure LooseForearm @18:10:23.25 Hand.close @18:10:23.99 SE.seek :down @18:10:24.19 Forearm.seek :retract @18:10:25.13 Elbow.seek CC @18:10:26.77 Elbow.reconfigure LooseElbow @18:10:26.86 Forearm.reconfigure TightForearm @18:10:26.90 Forearm.seek CC @18:10:27.00 SE.coast @18:10:28.22 CC.seek :closed @18:10:32.57 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.38 @18:10:32.58 Hand.open @18:10:33.41 Forearm.reconfigure LooseForearm @18:10:33.46 Forearm.seek :retract @18:10:34.49 SC.coast @18:10:34.53 collectSample 1500ml w/bubble point of 55psi @18:10:34.54 Priming 7ml @18:10:34.55 Gate.power :sampler,:ON @18:10:34.56 Intake.select :close @18:10:34.57 Exhaust.select :close @18:10:34.58 SS.hold @18:10:34.77 Calibrating pressure sensors @18:10:34.78 CTV.dial :air @18:10:36.30 SSV.dial :spit,avoiding: :exhaust @18:10:36.32 SS.reconfigure SSfast @18:10:36.37 SS.seek 5ml @18:10:39.12 SSV.dial CBV @18:10:39.15 CBV.dial SSV @18:10:40.74 delay 3.8 seconds @18:10:44.66 SSV.dial :spit @18:10:46.16 SS.seek 0ml @18:10:48.92 SSV.dial CBV @18:10:50.40 CTV.dial :intake @18:10:51.24 Waiting for external sampler to finish priming... @18:10:51.25 delayUntil "18:15:34.55" @18:13:38.08 delay 5 seconds @18:13:43.10 ATV.dial ASV @18:13:44.13 ASV.dial :SPEdelivery @18:13:44.15 AS.seek 0.02ml @18:15:34.56 SSV.dial CBV @18:15:34.59 CBV.dial SSV @18:15:34.60 Allowing 40s for 7.0ml of remaining 7.0ml @18:15:34.61 Intake.select :open @18:15:36.77 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:15:36.78 SS.reconfigure SSinhale.with maxSpeed: 150 @18:15:36.83 SS.seek 7ml @18:15:42.87 Stroke completed -- waiting for pressure to equalize ... @18:16:02.92 Intake.select :close @18:16:03.07 SSV.dial :exhaust @18:16:03.93 Exhaust.select :open @18:16:04.04 SS.reconfigure SSexhale @18:16:04.09 SS.seek 0ml @18:16:08.26 Exhaust.select :close @18:16:08.37 Sampling 1500.0ml @18:16:08.40 Intake.select :close @18:16:08.41 Exhaust.select :close @18:16:08.42 SS.reconfigure SSconfig @18:16:08.46 SS.hold @18:16:08.65 CTV.dial :intake @18:16:08.67 SSV.dial CBV @18:16:08.70 CBV.dial SSV @18:16:09.57 Allowing 130s for 25.0ml of remaining 1500.0ml @18:16:09.58 Intake.select :open @18:16:11.74 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:16:11.75 SS.reconfigure SSinhale.with maxSpeed: 150 @18:16:11.80 SS.seek 25ml @18:16:16.07 Vacuum too high -- waiting up to 40s @18:16:23.77 Intake @15.9psia, vacuum from 9.4 to 13.4psi @18:16:23.79 SS.reconfigure SSinhale.with maxSpeed: 135 @18:16:23.83 SS.seek 25ml @18:16:27.88 Vacuum too high -- waiting up to 40s @18:16:37.58 SS.reconfigure SSinhale.with maxSpeed: 122 @18:16:37.71 SS.seek 25ml @18:16:41.88 Vacuum too high -- waiting up to 40s @18:16:52.09 Intake @16.1psia, vacuum from 9.6 to 13.6psi @18:16:52.11 SS.reconfigure SSinhale.with maxSpeed: 110 @18:16:52.15 SS.seek 25ml @18:16:56.01 Vacuum too high -- waiting up to 40s @18:17:01.49 delay 5 seconds @18:17:06.28 Intake @16.0psia, vacuum from 9.5 to 13.5psi @18:17:06.30 SS.reconfigure SSinhale.with maxSpeed: 99 @18:17:06.34 SS.seek 25ml @18:17:06.51 ATV.dial :air2 @18:17:07.60 AS.seek 0.52ml @18:17:10.06 Stroke completed -- waiting for pressure to equalize ... @18:17:22.93 Intake.select :close @18:17:23.58 SSV.dial :exhaust @18:17:24.45 Exhaust.select :open @18:17:24.56 SS.reconfigure SSexhale @18:17:24.61 SS.seek 0ml @18:17:34.06 Exhaust.select :close @18:17:34.17 SSV.dial CBV @18:17:34.19 CBV.dial SSV @18:17:35.06 Allowing 130s for 25.0ml of remaining 1475.0ml @18:17:35.07 Intake.select :open @18:17:37.23 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:17:37.25 SS.reconfigure SSinhale.with maxSpeed: 99 @18:17:37.29 SS.seek 25ml @18:17:41.75 Vacuum too high -- waiting up to 40s @18:17:50.11 Intake @15.8psia, vacuum from 9.3 to 13.3psi @18:17:50.13 SS.reconfigure SSinhale.with maxSpeed: 89 @18:17:50.17 SS.seek 25ml @18:17:54.35 Vacuum too high -- waiting up to 40s @18:18:02.75 Intake @15.9psia, vacuum from 9.4 to 13.4psi @18:18:02.77 SS.reconfigure SSinhale.with maxSpeed: 80 @18:18:02.81 SS.seek 25ml @18:18:06.85 Vacuum too high -- waiting up to 40s @18:18:15.59 Intake @15.9psia, vacuum from 9.4 to 13.4psi @18:18:15.60 SS.reconfigure SSinhale.with maxSpeed: 72 @18:18:15.65 SS.seek 25ml @18:18:20.69 Vacuum too high -- waiting up to 40s @18:18:30.67 SS.reconfigure SSinhale.with maxSpeed: 65 @18:18:30.72 SS.seek 25ml @18:18:36.41 Vacuum too high -- waiting up to 40s @18:18:46.19 Intake @15.8psia, vacuum from 9.3 to 13.3psi @18:18:46.21 SS.reconfigure SSinhale.with maxSpeed: 59 @18:18:46.25 SS.seek 25ml @18:18:48.49 delay 5 seconds @18:18:49.31 Stroke completed -- waiting for pressure to equalize ... @18:18:53.58 ATV.dial ASV @18:18:54.62 ASV.dial :wasteR @18:18:55.00 AS.seek 0.3ml @18:18:58.58 Intake.select :close @18:18:58.73 SSV.dial :exhaust @18:18:59.57 Exhaust.select :open @18:18:59.68 SS.reconfigure SSexhale @18:18:59.73 SS.seek 0ml @18:19:09.23 Exhaust.select :close @18:19:09.34 SSV.dial CBV @18:19:09.36 CBV.dial SSV @18:19:10.23 Allowing 130s for 25.0ml of remaining 1450.0ml @18:19:10.24 Intake.select :open @18:19:12.40 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:19:12.42 SS.reconfigure SSinhale.with maxSpeed: 59 @18:19:12.46 SS.seek 25ml @18:19:19.16 Vacuum too high -- waiting up to 40s @18:19:26.87 Intake @15.8psia, vacuum from 9.3 to 13.3psi @18:19:26.89 SS.reconfigure SSinhale.with maxSpeed: 53 @18:19:26.93 SS.seek 25ml @18:19:33.72 Vacuum too high -- waiting up to 40s @18:19:40.01 delay 20 seconds @18:19:42.14 Intake @15.8psia, vacuum from 9.3 to 13.3psi @18:19:42.16 SS.reconfigure SSinhale.with maxSpeed: 48 @18:19:42.20 SS.seek 25ml @18:19:50.07 Vacuum too high -- waiting up to 40s @18:19:59.13 Intake @15.8psia, vacuum from 9.3 to 13.3psi @18:19:59.15 SS.reconfigure SSinhale.with maxSpeed: 43 @18:19:59.19 SS.seek 25ml @18:20:00.04 AS.seek 0.07ml @18:20:09.66 Vacuum too high -- waiting up to 40s @18:20:19.93 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:20:19.95 SS.reconfigure SSinhale.with maxSpeed: 39 @18:20:19.99 SS.seek 25ml @18:20:27.20 Stroke completed -- waiting for pressure to equalize ... @18:20:38.69 Intake.select :close @18:20:38.84 SSV.dial :exhaust @18:20:39.70 Exhaust.select :open @18:20:39.81 SS.reconfigure SSexhale @18:20:39.86 SS.seek 0ml @18:20:46.57 delay 20 seconds @18:20:49.65 Exhaust.select :close @18:20:49.76 SSV.dial CBV @18:20:49.78 CBV.dial SSV @18:20:50.65 Allowing 130s for 25.0ml of remaining 1425.0ml @18:20:50.66 Intake.select :open @18:20:52.83 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:20:52.85 SS.reconfigure SSinhale.with maxSpeed: 39 @18:20:52.89 SS.seek 25ml @18:21:05.19 Vacuum too high -- waiting up to 40s @18:21:06.60 ATV.dial :waste2 @18:21:07.38 AS.reconfigure ASconfig @18:21:07.43 AS.seek :empty @18:21:11.80 Elution @18:21:11.81 ATV.dial :air1 @18:21:12.69 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:21:12.71 SS.reconfigure SSinhale.with maxSpeed: 35 @18:21:12.75 SS.seek 25ml @18:21:12.99 AS.reconfigure ASslow1 @18:21:13.03 AS.seek 0.049ml @18:21:23.43 delay 3 seconds @18:21:26.44 Vacuum too high -- waiting up to 40s @18:21:26.47 ATV.dial :ElutionWater @18:21:27.23 AS.seek 0.109ml @18:21:35.11 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:21:35.13 SS.reconfigure SSinhale.with maxSpeed: 32 @18:21:35.17 SS.seek 25ml @18:21:39.57 delay 3 seconds @18:21:42.59 ATV.dial ASV,via: :air2 @18:21:44.84 ASV.dial :SPEelution @18:21:45.43 AFV.select :carrierSolution @18:21:45.58 AS.reconfigure ASnorm @18:21:45.63 AS.seek 0.759ml @18:21:57.82 Vacuum too high -- waiting up to 40s @18:22:09.24 SS.reconfigure SSinhale.with maxSpeed: 29 @18:22:09.28 SS.seek 25ml @18:22:13.74 Stroke completed -- waiting for pressure to equalize ... @18:22:18.66 delay 3 seconds @18:22:21.72 AFV.select :holdingCoil @18:22:21.78 Intake.select :close @18:22:21.87 AS.reconfigure ASslow1 @18:22:21.92 AS.seek 0.474ml @18:22:22.00 SSV.dial :exhaust @18:22:22.85 Exhaust.select :open @18:22:22.96 SS.reconfigure SSexhale @18:22:23.01 SS.seek 0ml @18:22:32.18 Exhaust.select :close @18:22:32.29 SSV.dial CBV @18:22:32.31 CBV.dial SSV @18:22:33.18 Allowing 130s for 25.0ml of remaining 1400.0ml @18:22:33.19 Intake.select :open @18:22:35.35 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:22:35.36 SS.reconfigure SSinhale.with maxSpeed: 29 @18:22:35.41 SS.seek 25ml @18:23:02.03 Vacuum too high -- waiting up to 40s @18:23:10.91 SS.reconfigure SSinhale.with maxSpeed: 26 @18:23:10.95 SS.seek 25ml @18:23:19.52 delay 5 seconds @18:23:24.60 AS.reconfigure ASslow2 @18:23:24.64 AS.seek 0.439ml @18:23:50.12 Stroke completed -- waiting for pressure to equalize ... @18:23:59.97 delay 1:00 @18:24:05.41 Intake.select :close @18:24:06.06 SSV.dial :exhaust @18:24:06.91 Exhaust.select :open @18:24:07.02 SS.reconfigure SSexhale @18:24:07.07 SS.seek 0ml @18:24:16.66 Exhaust.select :close @18:24:16.77 SSV.dial CBV @18:24:16.79 CBV.dial SSV @18:24:17.66 Allowing 130s for 25.0ml of remaining 1375.0ml @18:24:17.67 Intake.select :open @18:24:19.83 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:24:19.85 SS.reconfigure SSinhale.with maxSpeed: 26 @18:24:19.89 SS.seek 25ml @18:24:59.99 pushing eluate to holding coil @18:25:00.06 AS.seek 0.279ml @18:25:02.66 Vacuum too high -- waiting up to 40s @18:25:12.20 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:25:12.22 SS.reconfigure SSinhale.with maxSpeed: 23 @18:25:12.26 SS.seek 25ml @18:25:41.44 Stroke completed -- waiting for pressure to equalize ... @18:25:52.38 Intake.select :close @18:25:52.53 SSV.dial :exhaust @18:25:53.37 Exhaust.select :open @18:25:53.48 SS.reconfigure SSexhale @18:25:53.53 SS.seek 0ml @18:26:03.29 Exhaust.select :close @18:26:03.40 SSV.dial CBV @18:26:03.42 CBV.dial SSV @18:26:04.29 Allowing 130s for 25.0ml of remaining 1350.0ml @18:26:04.30 Intake.select :open @18:26:06.47 Intake @15.5psia, vacuum from 9.0 to 13.0psi @18:26:06.49 SS.reconfigure SSinhale.with maxSpeed: 23 @18:26:06.53 SS.seek 25ml @18:27:23.40 Stroke completed -- waiting for pressure to equalize ... @18:27:36.89 Intake.select :close @18:27:37.04 SSV.dial :exhaust @18:27:37.90 Exhaust.select :open @18:27:38.01 SS.reconfigure SSexhale @18:27:38.06 SS.seek 0ml @18:27:41.07 delay 30 seconds @18:27:47.71 Exhaust.select :close @18:27:47.82 SSV.dial CBV @18:27:47.84 CBV.dial SSV @18:27:48.71 Allowing 130s for 25.0ml of remaining 1325.0ml @18:27:48.72 Intake.select :open @18:27:50.88 Intake @15.7psia, vacuum from 9.2 to 13.2psi @18:27:50.89 SS.reconfigure SSinhale.with maxSpeed: 23 @18:27:50.94 SS.seek 25ml @18:28:11.09 ATV.dial :waste,via: :air3 @18:28:12.93 AS.reconfigure ASconfig @18:28:12.98 AS.seek :empty @18:28:29.36 Parking Bleach in LysateSource line and SPEcolumn @18:28:29.37 ATV.dial :air3 @18:28:30.24 AS.reconfigure ASslow1 @18:28:30.77 AS.seek 0.029ml @18:28:37.13 ATV.dial :ElutionWater @18:28:37.75 AS.seek 0.059ml @18:28:44.04 ATV.dial :air3 @18:28:44.67 AS.seek 0.069ml @18:28:46.93 delay 2 seconds @18:28:48.95 ATV.dial :bleach @18:28:49.41 AS.seek 0.769ml @18:29:08.22 Stroke completed -- waiting for pressure to equalize ... @18:29:24.15 Intake.select :close @18:29:24.30 SSV.dial :exhaust @18:29:25.15 Exhaust.select :open @18:29:25.26 SS.reconfigure SSexhale @18:29:25.31 SS.seek 0ml @18:29:34.91 Exhaust.select :close @18:29:35.02 SSV.dial CBV @18:29:35.04 CBV.dial SSV @18:29:35.91 Allowing 130s for 25.0ml of remaining 1300.0ml @18:29:35.92 Intake.select :open @18:29:38.08 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:29:38.10 SS.reconfigure SSinhale.with maxSpeed: 23 @18:29:38.14 SS.seek 25ml @18:30:55.16 Stroke completed -- waiting for pressure to equalize ... @18:31:10.46 delay 3 seconds @18:31:12.29 Intake.select :close @18:31:12.44 SSV.dial :exhaust @18:31:13.30 Exhaust.select :open @18:31:13.41 SS.reconfigure SSexhale @18:31:13.46 SS.seek 0ml @18:31:13.50 ATV.dial :air3 @18:31:14.07 delay 3 seconds @18:31:17.14 AS.seek 0.794ml @18:31:22.36 delay 3 seconds @18:31:23.30 Exhaust.select :close @18:31:23.41 SSV.dial CBV @18:31:23.43 CBV.dial SSV @18:31:24.30 Allowing 130s for 25.0ml of remaining 1275.0ml @18:31:24.31 Intake.select :open @18:31:25.38 ATV.dial :bleach @18:31:26.00 AS.seek 0.779ml @18:31:26.47 Intake @15.6psia, vacuum from 9.1 to 13.1psi @18:31:26.49 SS.reconfigure SSinhale.with maxSpeed: 23 @18:31:26.53 SS.seek 25ml @18:31:29.30 delay 3 seconds @18:31:32.32 ATV.dial :AIV @18:31:33.65 AS.reconfigure ASconfig @18:31:33.70 AS.seek 0.015ml @18:32:17.91 ATV.dial :waste @18:32:19.49 AS.seek :empty @18:32:20.68 ATV.dial :air3 @18:32:21.39 AS.reconfigure ASslow1 @18:32:21.43 AS.seek 0.009ml @18:32:23.74 delay 2 seconds @18:32:25.76 ATV.dial :bleach @18:32:26.22 AS.seek 0.579ml @18:32:40.72 Vacuum too high -- waiting up to 40s @18:32:51.67 SS.reconfigure SSinhale.with maxSpeed: 21 @18:32:51.72 SS.seek 25ml @18:32:54.93 Stroke completed -- waiting for pressure to equalize ... @18:33:01.11 Intake.select :close @18:33:01.25 SSV.dial :exhaust @18:33:02.11 Exhaust.select :open @18:33:02.22 SS.reconfigure SSexhale @18:33:02.27 SS.seek 0ml @18:33:11.85 Exhaust.select :close @18:33:11.96 SSV.dial CBV @18:33:11.98 CBV.dial SSV @18:33:12.85 Allowing 130s for 25.0ml of remaining 1250.0ml @18:33:12.86 Intake.select :open @18:33:15.02 Intake @15.8psia, vacuum from 9.3 to 13.3psi @18:33:15.04 SS.reconfigure SSinhale.with maxSpeed: 21 @18:33:15.08 SS.seek 25ml @18:34:21.02 delay 3 seconds @18:34:24.04 ATV.dial :air3 @18:34:24.61 delay 3 seconds @18:34:27.68 AS.seek 0.604ml @18:34:33.08 delay 3 seconds @18:34:36.10 ATV.dial :bleach @18:34:36.72 AS.seek 0.589ml @18:34:40.01 delay 3 seconds @18:34:40.40 Stroke completed -- waiting for pressure to equalize ... @18:34:43.03 ATV.dial :ASV @18:34:44.15 ASV.dial :wasteR @18:34:45.24 AS.seek 0ml @18:34:54.60 Intake.select :close @18:34:54.75 SSV.dial :exhaust @18:34:55.60 Exhaust.select :open @18:34:55.71 SS.reconfigure SSexhale @18:34:55.76 SS.seek 0ml @18:35:05.46 Exhaust.select :close @18:35:05.57 SSV.dial CBV @18:35:05.59 CBV.dial SSV @18:35:06.44 Allowing 130s for 25.0ml of remaining 1225.0ml @18:35:06.45 Intake.select :open @18:35:08.61 Intake @15.8psia, vacuum from 9.3 to 13.3psi @18:35:08.62 SS.reconfigure SSinhale.with maxSpeed: 21 @18:35:08.67 SS.seek 25ml @18:36:33.32 Stroke completed -- waiting for pressure to equalize ... @18:36:43.98 delay 1:00 @18:36:47.40 Intake.select :close @18:36:47.55 SSV.dial :exhaust @18:36:48.39 Exhaust.select :open @18:36:48.50 SS.reconfigure SSexhale @18:36:48.55 SS.seek 0ml @18:36:58.24 Exhaust.select :close @18:36:58.35 SSV.dial CBV @18:36:58.37 CBV.dial SSV @18:36:59.24 Allowing 130s for 25.0ml of remaining 1200.0ml @18:36:59.25 Intake.select :open @18:37:01.41 Intake @15.8psia, vacuum from 9.3 to 13.3psi @18:37:01.42 SS.reconfigure SSinhale.with maxSpeed: 21 @18:37:01.47 SS.seek 25ml @18:37:44.00 AFV.select :carrierSolution @18:37:44.10 delay 2 seconds @18:37:46.13 AS.reconfigure ASconfig @18:37:46.17 AS.seek 1ml @18:38:26.78 Stroke completed -- waiting for pressure to equalize ... @18:38:42.71 Intake.select :close @18:38:42.86 SSV.dial :exhaust @18:38:43.72 Exhaust.select :open @18:38:43.83 SS.reconfigure SSexhale @18:38:43.88 SS.seek 0ml @18:38:43.94 delay 1 second @18:38:44.97 AFV.select :holdingCoil @18:38:45.07 delay 1 second @18:38:46.09 ATV.dial ASV @18:38:46.10 ASV.dial :wasteR @18:38:46.12 AS.reconfigure ASslow1 @18:38:46.17 AS.seek 0ml @18:38:53.57 Exhaust.select :close @18:38:53.68 SSV.dial CBV @18:38:53.70 CBV.dial SSV @18:38:54.57 Allowing 130s for 25.0ml of remaining 1175.0ml @18:38:54.58 Intake.select :open @18:38:56.74 Intake @15.8psia, vacuum from 9.3 to 13.3psi @18:38:56.76 SS.reconfigure SSinhale.with maxSpeed: 21 @18:38:56.80 SS.seek 25ml @18:40:21.75 Stroke completed -- waiting for pressure to equalize ... @18:40:36.92 Intake.select :close @18:40:37.07 SSV.dial :exhaust @18:40:37.91 Exhaust.select :open @18:40:38.02 SS.reconfigure SSexhale @18:40:38.07 SS.seek 0ml @18:40:47.69 Exhaust.select :close @18:40:47.80 SSV.dial CBV @18:40:47.82 CBV.dial SSV @18:40:48.69 Allowing 130s for 25.0ml of remaining 1150.0ml @18:40:48.70 Intake.select :open @18:40:50.86 Intake @15.8psia, vacuum from 9.3 to 13.3psi @18:40:50.88 SS.reconfigure SSinhale.with maxSpeed: 21 @18:40:50.92 SS.seek 25ml @18:42:07.52 delay 20 seconds @18:42:15.63 Stroke completed -- waiting for pressure to equalize ... @18:42:27.54 ATV.dial :air3 @18:42:29.14 AS.seek 0.59ml @18:42:32.79 Intake.select :close @18:42:32.94 SSV.dial :exhaust @18:42:33.80 Exhaust.select :open @18:42:33.91 SS.reconfigure SSexhale @18:42:33.96 SS.seek 0ml @18:42:43.56 Exhaust.select :close @18:42:43.67 SSV.dial CBV @18:42:43.69 CBV.dial SSV @18:42:44.56 Allowing 130s for 25.0ml of remaining 1125.0ml @18:42:44.57 Intake.select :open @18:42:46.73 Intake @15.9psia, vacuum from 9.4 to 13.4psi @18:42:46.75 SS.reconfigure SSinhale.with maxSpeed: 21 @18:42:46.79 SS.seek 25ml @18:44:11.52 Stroke completed -- waiting for pressure to equalize ... @18:44:28.10 delay 3 seconds @18:44:28.56 Intake.select :close @18:44:28.71 SSV.dial :exhaust @18:44:29.55 Exhaust.select :open @18:44:29.66 SS.reconfigure SSexhale @18:44:29.71 SS.seek 0ml @18:44:31.12 ATV.dial :ASV @18:44:32.58 ASV.dial :wasteR @18:44:32.65 AS.seek 0.09ml @18:44:39.38 Exhaust.select :close @18:44:39.49 SSV.dial CBV @18:44:39.51 CBV.dial SSV @18:44:40.38 Allowing 130s for 25.0ml of remaining 1100.0ml @18:44:40.39 Intake.select :open @18:44:42.55 Intake @15.9psia, vacuum from 9.4 to 13.4psi @18:44:42.56 SS.reconfigure SSinhale.with maxSpeed: 21 @18:44:42.61 SS.seek 25ml @18:46:07.48 Stroke completed -- waiting for pressure to equalize ... @18:46:13.49 delay 30 seconds @18:46:23.91 Intake.select :close @18:46:24.06 SSV.dial :exhaust @18:46:24.92 Exhaust.select :open @18:46:25.03 SS.reconfigure SSexhale @18:46:25.08 SS.seek 0ml @18:46:34.84 Exhaust.select :close @18:46:34.95 SSV.dial CBV @18:46:34.97 CBV.dial SSV @18:46:35.84 Allowing 130s for 25.0ml of remaining 1075.0ml @18:46:35.85 Intake.select :open @18:46:38.02 Intake @15.9psia, vacuum from 9.4 to 13.4psi @18:46:38.04 SS.reconfigure SSinhale.with maxSpeed: 21 @18:46:38.08 SS.seek 25ml @18:46:43.51 ATV.dial :extSrc @18:46:44.30 delay 3 seconds @18:46:47.32 ATV.dial :ElutionWater @18:46:48.50 AS.seek 0.12ml @18:46:54.80 ATV.dial :air3 @18:46:55.42 AS.seek 0.13ml @18:46:57.71 delay 2 seconds @18:46:59.73 ATV.dial :bleach @18:47:00.22 AS.seek 0.23ml @18:47:20.63 delay 3 seconds @18:47:23.65 ATV.dial :air3 @18:47:24.22 delay 3 seconds @18:47:27.29 AS.seek 0.255ml @18:47:32.60 delay 3 seconds @18:47:35.62 ATV.dial :bleach @18:47:36.25 AS.seek 0.24ml @18:47:39.55 delay 3 seconds @18:47:42.57 ATV.dial :ASV @18:47:43.68 ASV.dial :SPEdelivery @18:47:44.05 AFV.select :carrierSolution @18:47:44.15 delay 2 seconds @18:47:46.18 AS.reconfigure ASconfig @18:47:46.22 AS.seek 0.5ml @18:48:01.46 delay 1 second @18:48:02.48 AFV.select :holdingCoil @18:48:02.63 AS.reconfigure ASslow1 @18:48:02.67 AS.seek 0.17ml @18:48:02.80 Stroke completed -- waiting for pressure to equalize ... @18:48:20.40 Intake.select :close @18:48:20.55 SSV.dial :exhaust @18:48:21.39 Exhaust.select :open @18:48:21.50 SS.reconfigure SSexhale @18:48:21.55 SS.seek 0ml @18:48:31.28 Exhaust.select :close @18:48:31.39 SSV.dial CBV @18:48:31.41 CBV.dial SSV @18:48:32.28 Allowing 130s for 25.0ml of remaining 1050.0ml @18:48:32.29 Intake.select :open @18:48:34.46 Intake @15.9psia, vacuum from 9.4 to 13.4psi @18:48:34.48 SS.reconfigure SSinhale.with maxSpeed: 21 @18:48:34.52 SS.seek 25ml @18:49:09.28 SPE.hold 2:46:40,95C @18:49:09.32 delay 3:00 @18:49:59.39 Stroke completed -- waiting for pressure to equalize ... @18:50:16.55 Intake.select :close @18:50:16.70 SSV.dial :exhaust @18:50:17.59 Exhaust.select :open @18:50:17.70 SS.reconfigure SSexhale @18:50:17.75 SS.seek 0ml @18:50:27.69 Exhaust.select :close @18:50:27.80 SSV.dial CBV @18:50:27.82 CBV.dial SSV @18:50:28.72 Allowing 130s for 25.0ml of remaining 1025.0ml @18:50:28.73 Intake.select :open @18:50:30.89 Intake @15.9psia, vacuum from 9.4 to 13.4psi @18:50:30.91 SS.reconfigure SSinhale.with maxSpeed: 21 @18:50:30.95 SS.seek 25ml @18:51:55.64 Stroke completed -- waiting for pressure to equalize ... @18:52:09.34 AFV.select :carrierSolution @18:52:09.44 delay 2 seconds @18:52:11.47 AS.reconfigure ASconfig @18:52:11.52 AS.seek 1ml @18:52:13.27 Intake.select :close @18:52:13.42 SSV.dial :exhaust @18:52:14.30 Exhaust.select :open @18:52:14.41 SS.reconfigure SSexhale @18:52:14.46 SS.seek 0ml @18:52:24.19 Exhaust.select :close @18:52:24.30 SSV.dial CBV @18:52:24.32 CBV.dial SSV @18:52:25.22 Allowing 130s for 25.0ml of remaining 1000.0ml @18:52:25.23 Intake.select :open @18:52:27.39 Intake @16.0psia, vacuum from 9.5 to 13.5psi @18:52:27.41 SS.reconfigure SSinhale.with maxSpeed: 21 @18:52:27.45 SS.seek 25ml @18:52:59.49 delay 1 second @18:53:00.51 AFV.select :holdingCoil @18:53:00.61 delay 1 second @18:53:01.63 ASV.dial :wasteD @18:53:02.73 AS.seek 0ml @18:53:52.44 Stroke completed -- waiting for pressure to equalize ... @18:54:00.51 Priming Eluate @18:54:00.52 AFV.select :carrierSolution @18:54:00.62 delay 2 seconds @18:54:02.65 AS.seek 0.5ml @18:54:10.70 Intake.select :close @18:54:10.85 SSV.dial :exhaust @18:54:11.73 Exhaust.select :open @18:54:11.84 SS.reconfigure SSexhale @18:54:11.89 SS.seek 0ml @18:54:21.59 Exhaust.select :close @18:54:21.70 SSV.dial CBV @18:54:21.72 CBV.dial SSV @18:54:22.62 Allowing 130s for 25.0ml of remaining 975.0ml @18:54:22.63 Intake.select :open @18:54:24.79 Intake @16.0psia, vacuum from 9.5 to 13.5psi @18:54:24.81 SS.reconfigure SSinhale.with maxSpeed: 21 @18:54:24.85 SS.seek 25ml @18:54:31.66 delay 1 second @18:54:32.68 AFV.select :holdingCoil @18:54:32.78 ATV.dial ASV @18:54:32.80 ASV.dial :SPErecovery @18:54:33.41 Swish #1 of 2 @18:54:33.48 AS.reconfigure ASslow0 @18:54:33.52 AS.seek 0.6ml @18:54:43.97 AS.seek 0.5ml @18:54:54.32 Swish #2 of 2 @18:54:54.39 AS.seek 0.6ml @18:55:04.85 AS.seek 0.5ml @18:55:15.21 delay 3 seconds @18:55:18.28 AS.reconfigure ASslow1 @18:55:18.33 AS.seek 0.684ml @18:55:49.79 Stroke completed -- waiting for pressure to equalize ... @18:55:55.65 delay 3 seconds @18:55:58.67 Eluate primed @18:55:58.68 ATV.dial :waste2 @18:55:59.33 AS.reconfigure ASnorm @18:55:59.38 AS.seek 0ml @18:56:08.65 Intake.select :close @18:56:08.80 SSV.dial :exhaust @18:56:09.67 Exhaust.select :open @18:56:09.78 SS.reconfigure SSexhale @18:56:09.83 SS.seek 0ml @18:56:19.64 Exhaust.select :close @18:56:19.75 SSV.dial CBV @18:56:19.77 CBV.dial SSV @18:56:20.65 Allowing 130s for 25.0ml of remaining 950.0ml @18:56:20.66 Intake.select :open @18:56:22.83 Intake @16.0psia, vacuum from 9.5 to 13.5psi @18:56:22.85 SS.reconfigure SSinhale.with maxSpeed: 21 @18:56:22.89 SS.seek 25ml @18:56:34.19 SPE.coast @18:56:34.26 ASV.dial :SPErecovery @18:56:34.28 Setting up PCR for IPc -- writing /var/log/esp/IPc19may1718h1000ml.pcr @18:56:34.29 Building PCR Slug from ASV with MM1 mix, PP1 primer @18:56:34.30 Priming with carrier @18:56:34.31 ATV.dial ARV @18:56:35.33 ARV.dial :carrier @18:56:35.68 AS.seek 0.5ml @18:57:01.25 AFV.select :carrierSolution @18:57:01.35 delay 2 seconds @18:57:03.38 AS.reconfigure ASconfig @18:57:03.42 AS.seek 1ml @18:57:32.44 delay 1 second @18:57:33.46 AFV.select :holdingCoil @18:57:33.56 delay 1 second @18:57:34.58 ATV.dial :waste @18:57:36.18 AS.reconfigure ASnorm @18:57:36.23 AS.seek :empty @18:57:47.64 Stroke completed -- waiting for pressure to equalize ... @18:58:06.01 Intake.select :close @18:58:06.16 SSV.dial :exhaust @18:58:07.01 Exhaust.select :open @18:58:07.12 SS.reconfigure SSexhale @18:58:07.17 SS.seek 0ml @18:58:16.90 Exhaust.select :close @18:58:17.01 SSV.dial CBV @18:58:17.03 CBV.dial SSV @18:58:18.39 Allowing 130s for 25.0ml of remaining 925.0ml @18:58:18.40 Intake.select :open @18:58:20.56 Intake @16.1psia, vacuum from 9.6 to 13.6psi @18:58:20.58 SS.reconfigure SSinhale.with maxSpeed: 21 @18:58:20.62 SS.seek 25ml @18:58:26.96 AFV.select :carrierSolution @18:58:27.07 delay 2 seconds @18:58:29.09 AS.reconfigure ASconfig @18:58:29.14 AS.seek 0.5ml @18:58:58.24 delay 1 second @18:58:59.26 AFV.select :holdingCoil @18:58:59.37 Building Slug @18:58:59.38 ATV.dial :ARV @18:59:01.10 delay 2 seconds @18:59:03.12 ARV.dial :air1 @18:59:03.52 delay 2 seconds @18:59:05.59 AS.reconfigure ASslow2 @18:59:05.64 AS.seek 0.506ml @18:59:11.96 delay 2 seconds @18:59:13.98 ARV.dial :MM1 @18:59:14.60 delay 2 seconds @18:59:16.63 Adding MM1 @18:59:16.69 AS.seek 0.524ml @18:59:34.97 delay 25 seconds @18:59:44.59 Vacuum too high -- waiting up to 40s @18:59:56.70 Intake @15.9psia, vacuum from 9.4 to 13.4psi @18:59:56.72 SS.reconfigure SSinhale.with maxSpeed: 19 @18:59:56.76 SS.seek 25ml @18:59:58.06 Stroke completed -- waiting for pressure to equalize ... @18:59:59.99 ARV.dial :PP1 @19:00:00.38 Adding PP1 @19:00:00.40 delay 2 seconds @19:00:02.46 AS.seek 0.53ml @19:00:04.22 Intake.select :close @19:00:04.37 SSV.dial :exhaust @19:00:05.23 Exhaust.select :open @19:00:05.34 SS.reconfigure SSexhale @19:00:05.39 SS.seek 0ml @19:00:08.97 delay 10 seconds @19:00:14.93 Exhaust.select :close @19:00:15.04 SSV.dial CBV @19:00:15.06 CBV.dial SSV @19:00:15.93 Allowing 130s for 25.0ml of remaining 900.0ml @19:00:15.94 Intake.select :open @19:00:18.10 Intake @16.1psia, vacuum from 9.6 to 13.6psi @19:00:18.12 SS.reconfigure SSinhale.with maxSpeed: 19 @19:00:18.16 SS.seek 25ml @19:00:18.99 ARV.dial :air1 @19:00:19.38 adding ARV air @19:00:19.40 delay 2 seconds @19:00:21.46 AS.seek 0.536ml @19:00:27.85 delay 5 seconds @19:00:32.87 ARV.dial :carrier @19:00:33.27 delay 7 seconds @19:00:40.35 AS.seek 0.594ml @19:01:38.93 delay 5 seconds @19:01:43.95 ATV.dial :ASV @19:01:44.36 delay 2 seconds @19:01:46.43 AS.seek 0.6ml @19:01:52.28 Stroke completed -- waiting for pressure to equalize ... @19:01:52.91 delay 4 seconds @19:01:56.94 ATV.dial ARV @19:01:57.51 delay 2 seconds @19:01:59.53 ARV.dial :carrier @19:01:59.55 delay 2 seconds @19:02:02.06 AS.seek 0.65ml @19:02:07.55 Intake.select :close @19:02:07.70 SSV.dial :exhaust @19:02:08.56 Exhaust.select :open @19:02:08.67 SS.reconfigure SSexhale @19:02:08.72 SS.seek 0ml @19:02:18.04 Exhaust.select :close @19:02:18.15 SSV.dial CBV @19:02:18.17 CBV.dial SSV @19:02:19.03 Allowing 130s for 25.0ml of remaining 875.0ml @19:02:19.04 Intake.select :open @19:02:21.20 Intake @16.1psia, vacuum from 9.6 to 13.6psi @19:02:21.22 SS.reconfigure SSinhale.with maxSpeed: 19 @19:02:21.26 SS.seek 25ml @19:02:52.69 delay 4 seconds @19:02:56.71 ATV.dial PCR @19:02:57.35 ASV.dial :waste1 @19:02:58.14 ARV.dial :air1 @19:02:58.54 Positioning Slug by 0.324ml @19:02:58.62 AS.seek 0.326ml @19:03:56.03 Stroke completed -- waiting for pressure to equalize ... @19:04:11.98 Intake.select :close @19:04:12.13 SSV.dial :exhaust @19:04:12.98 Exhaust.select :open @19:04:13.09 SS.reconfigure SSexhale @19:04:13.14 SS.seek 0ml @19:04:22.96 Exhaust.select :close @19:04:23.07 SSV.dial CBV @19:04:23.09 CBV.dial SSV @19:04:24.48 Allowing 130s for 25.0ml of remaining 850.0ml @19:04:24.49 Intake.select :open @19:04:26.65 Intake @16.1psia, vacuum from 9.6 to 13.6psi @19:04:26.67 SS.reconfigure SSinhale.with maxSpeed: 19 @19:04:26.71 SS.seek 25ml @19:06:01.06 Stroke completed -- waiting for pressure to equalize ... @19:06:15.15 Intake.select :close @19:06:15.30 SSV.dial :exhaust @19:06:16.15 Exhaust.select :open @19:06:16.26 SS.reconfigure SSexhale @19:06:16.31 SS.seek 0ml @19:06:25.96 Exhaust.select :close @19:06:26.07 SSV.dial CBV @19:06:26.09 CBV.dial SSV @19:06:26.95 Allowing 130s for 25.0ml of remaining 825.0ml @19:06:26.96 Intake.select :open @19:06:29.12 Intake @16.1psia, vacuum from 9.6 to 13.6psi @19:06:29.13 SS.reconfigure SSinhale.with maxSpeed: 19 @19:06:29.18 SS.seek 25ml @19:08:03.42 Stroke completed -- waiting for pressure to equalize ... @19:08:19.26 Intake.select :close @19:08:19.41 SSV.dial :exhaust @19:08:20.26 Exhaust.select :open @19:08:20.37 SS.reconfigure SSexhale @19:08:20.42 SS.seek 0ml @19:08:24.69 delay 5 seconds @19:08:29.71 ATV.dial ARV @19:08:30.07 Exhaust.select :close @19:08:30.18 SSV.dial CBV @19:08:30.20 CBV.dial SSV @19:08:30.50 ASV.dial :PCRlock @19:08:30.88 delay 5 seconds @19:08:31.06 Allowing 130s for 25.0ml of remaining 800.0ml @19:08:31.07 Intake.select :open @19:08:33.24 Intake @16.1psia, vacuum from 9.6 to 13.6psi @19:08:33.26 SS.reconfigure SSinhale.with maxSpeed: 19 @19:08:33.30 SS.seek 25ml @19:08:35.91 Running IPc ProfileIPc thermocycling @19:08:35.92 PCR.program ProfileIPc @19:08:36.06 ProfileIPc should complete in < 1:50:49 @19:08:36.08 PCR.run 2:23:31.25 @19:10:07.31 Stroke completed -- waiting for pressure to equalize ... @19:10:23.85 Intake.select :close @19:10:24.00 SSV.dial :exhaust @19:10:24.84 Exhaust.select :open @19:10:24.95 SS.reconfigure SSexhale @19:10:25.00 SS.seek 0ml @19:10:34.65 Exhaust.select :close @19:10:34.76 SSV.dial CBV @19:10:34.78 CBV.dial SSV @19:10:35.65 Allowing 130s for 25.0ml of remaining 775.0ml @19:10:35.66 Intake.select :open @19:10:37.82 Intake @16.1psia, vacuum from 9.6 to 13.6psi @19:10:37.84 SS.reconfigure SSinhale.with maxSpeed: 19 @19:10:38.39 SS.seek 25ml @19:12:12.74 Stroke completed -- waiting for pressure to equalize ... @19:12:29.31 Intake.select :close @19:12:29.46 SSV.dial :exhaust @19:12:30.31 Exhaust.select :open @19:12:30.42 SS.reconfigure SSexhale @19:12:30.47 SS.seek 0ml @19:12:40.12 Exhaust.select :close @19:12:40.23 SSV.dial CBV @19:12:40.25 CBV.dial SSV @19:12:41.10 Allowing 130s for 25.0ml of remaining 750.0ml @19:12:41.11 Intake.select :open @19:12:43.27 Intake @16.1psia, vacuum from 9.6 to 13.6psi @19:12:43.28 SS.reconfigure SSinhale.with maxSpeed: 19 @19:12:43.33 SS.seek 25ml @19:14:17.32 Stroke completed -- waiting for pressure to equalize ... @19:14:33.78 Intake.select :close @19:14:33.93 SSV.dial :exhaust @19:14:34.79 Exhaust.select :open @19:14:34.90 SS.reconfigure SSexhale @19:14:34.95 SS.seek 0ml @19:14:44.84 Exhaust.select :close @19:14:44.95 SSV.dial CBV @19:14:44.97 CBV.dial SSV @19:14:45.85 Allowing 130s for 25.0ml of remaining 725.0ml @19:14:45.86 Intake.select :open @19:14:48.02 Intake @16.2psia, vacuum from 9.7 to 13.7psi @19:14:48.04 SS.reconfigure SSinhale.with maxSpeed: 19 @19:14:48.08 SS.seek 25ml @19:16:22.15 Stroke completed -- waiting for pressure to equalize ... @19:16:38.53 Intake.select :close @19:16:38.76 SSV.dial :exhaust @19:16:39.62 Exhaust.select :open @19:16:39.73 SS.reconfigure SSexhale @19:16:39.78 SS.seek 0ml @19:16:49.69 Exhaust.select :close @19:16:49.80 SSV.dial CBV @19:16:49.82 CBV.dial SSV @19:16:50.69 Allowing 130s for 25.0ml of remaining 700.0ml @19:16:50.70 Intake.select :open @19:16:52.87 Intake @16.1psia, vacuum from 9.6 to 13.6psi @19:16:52.89 SS.reconfigure SSinhale.with maxSpeed: 19 @19:16:52.93 SS.seek 25ml @19:18:26.55 Stroke completed -- waiting for pressure to equalize ... @19:18:42.49 Intake.select :close @19:18:42.64 SSV.dial :exhaust @19:18:43.48 Exhaust.select :open @19:18:43.59 SS.reconfigure SSexhale @19:18:43.64 SS.seek 0ml @19:18:53.38 Exhaust.select :close @19:18:53.49 SSV.dial CBV @19:18:53.51 CBV.dial SSV @19:18:54.38 Allowing 130s for 25.0ml of remaining 675.0ml @19:18:54.39 Intake.select :open @19:18:56.55 Intake @16.2psia, vacuum from 9.7 to 13.7psi @19:18:56.57 SS.reconfigure SSinhale.with maxSpeed: 19 @19:18:56.61 SS.seek 25ml @19:20:30.72 Stroke completed -- waiting for pressure to equalize ... @19:20:47.17 Intake.select :close @19:20:47.32 SSV.dial :exhaust @19:20:48.18 Exhaust.select :open @19:20:48.29 SS.reconfigure SSexhale @19:20:48.34 SS.seek 0ml @19:20:58.22 Exhaust.select :close @19:20:58.33 SSV.dial CBV @19:20:58.35 CBV.dial SSV @19:20:59.22 Allowing 130s for 25.0ml of remaining 650.0ml @19:20:59.23 Intake.select :open @19:21:01.39 Intake @16.2psia, vacuum from 9.7 to 13.7psi @19:21:01.41 SS.reconfigure SSinhale.with maxSpeed: 19 @19:21:01.45 SS.seek 25ml @19:22:35.43 Stroke completed -- waiting for pressure to equalize ... @19:22:51.46 Intake.select :close @19:22:51.61 SSV.dial :exhaust @19:22:52.46 Exhaust.select :open @19:22:52.57 SS.reconfigure SSexhale @19:22:52.62 SS.seek 0ml @19:23:02.60 Exhaust.select :close @19:23:02.71 SSV.dial CBV @19:23:02.73 CBV.dial SSV @19:23:03.58 Allowing 130s for 25.0ml of remaining 625.0ml @19:23:03.59 Intake.select :open @19:23:05.75 Intake @16.2psia, vacuum from 9.7 to 13.7psi @19:23:05.77 SS.reconfigure SSinhale.with maxSpeed: 19 @19:23:05.81 SS.seek 25ml @19:24:39.36 Stroke completed -- waiting for pressure to equalize ... @19:24:55.31 Intake.select :close @19:24:55.46 SSV.dial :exhaust @19:24:56.30 Exhaust.select :open @19:24:56.41 SS.reconfigure SSexhale @19:24:56.46 SS.seek 0ml @19:25:06.07 Exhaust.select :close @19:25:06.18 SSV.dial CBV @19:25:06.20 CBV.dial SSV @19:25:07.05 Allowing 130s for 25.0ml of remaining 600.0ml @19:25:07.06 Intake.select :open @19:25:09.22 Intake @16.3psia, vacuum from 9.8 to 13.8psi @19:25:09.24 SS.reconfigure SSinhale.with maxSpeed: 19 @19:25:09.28 SS.seek 25ml @19:26:43.63 Stroke completed -- waiting for pressure to equalize ... @19:27:01.55 Intake.select :close @19:27:01.70 SSV.dial :exhaust @19:27:02.55 Exhaust.select :open @19:27:02.66 SS.reconfigure SSexhale @19:27:02.71 SS.seek 0ml @19:27:12.39 Exhaust.select :close @19:27:12.50 SSV.dial CBV @19:27:12.53 CBV.dial SSV @19:27:13.39 Allowing 130s for 25.0ml of remaining 575.0ml @19:27:13.40 Intake.select :open @19:27:15.57 Intake @16.2psia, vacuum from 9.7 to 13.7psi @19:27:15.59 SS.reconfigure SSinhale.with maxSpeed: 19 @19:27:15.63 SS.seek 25ml @19:28:49.49 Stroke completed -- waiting for pressure to equalize ... @19:29:06.56 Intake.select :close @19:29:06.71 SSV.dial :exhaust @19:29:07.57 Exhaust.select :open @19:29:07.68 SS.reconfigure SSexhale @19:29:07.73 SS.seek 0ml @19:29:17.41 Exhaust.select :close @19:29:17.52 SSV.dial CBV @19:29:17.54 CBV.dial SSV @19:29:18.41 Allowing 130s for 25.0ml of remaining 550.0ml @19:29:18.42 Intake.select :open @19:29:20.59 Intake @16.2psia, vacuum from 9.7 to 13.7psi @19:29:20.61 SS.reconfigure SSinhale.with maxSpeed: 19 @19:29:20.65 SS.seek 25ml @19:30:54.83 Stroke completed -- waiting for pressure to equalize ... @19:31:11.91 Intake.select :close @19:31:12.07 SSV.dial :exhaust @19:31:12.91 Exhaust.select :open @19:31:13.02 SS.reconfigure SSexhale @19:31:13.07 SS.seek 0ml @19:31:22.83 Exhaust.select :close @19:31:22.94 SSV.dial CBV @19:31:22.96 CBV.dial SSV @19:31:23.82 Allowing 130s for 25.0ml of remaining 525.0ml @19:31:23.83 Intake.select :open @19:31:26.00 Intake @16.2psia, vacuum from 9.7 to 13.7psi @19:31:26.02 SS.reconfigure SSinhale.with maxSpeed: 19 @19:31:26.06 SS.seek 25ml @19:32:59.99 Stroke completed -- waiting for pressure to equalize ... @19:33:17.74 Intake.select :close @19:33:17.89 SSV.dial :exhaust @19:33:18.75 Exhaust.select :open @19:33:18.86 SS.reconfigure SSexhale @19:33:18.91 SS.seek 0ml @19:33:28.77 Exhaust.select :close @19:33:28.88 SSV.dial CBV @19:33:28.90 CBV.dial SSV @19:33:29.77 Allowing 130s for 25.0ml of remaining 500.0ml @19:33:29.78 Intake.select :open @19:33:31.94 Intake @16.2psia, vacuum from 9.7 to 13.7psi @19:33:31.96 SS.reconfigure SSinhale.with maxSpeed: 19 @19:33:32.00 SS.seek 25ml @19:35:06.20 Stroke completed -- waiting for pressure to equalize ... @19:35:22.74 Intake.select :close @19:35:22.89 SSV.dial :exhaust @19:35:23.75 Exhaust.select :open @19:35:23.86 SS.reconfigure SSexhale @19:35:23.91 SS.seek 0ml @19:35:33.67 Exhaust.select :close @19:35:33.78 SSV.dial CBV @19:35:33.80 CBV.dial SSV @19:35:34.67 Allowing 130s for 25.0ml of remaining 475.0ml @19:35:34.68 Intake.select :open @19:35:36.84 Intake @16.3psia, vacuum from 9.8 to 13.8psi @19:35:36.86 SS.reconfigure SSinhale.with maxSpeed: 19 @19:35:36.90 SS.seek 25ml @19:37:11.09 Stroke completed -- waiting for pressure to equalize ... @19:37:28.16 Intake.select :close @19:37:28.32 SSV.dial :exhaust @19:37:29.16 Exhaust.select :open @19:37:29.27 SS.reconfigure SSexhale @19:37:29.32 SS.seek 0ml @19:37:39.19 Exhaust.select :close @19:37:39.30 SSV.dial CBV @19:37:39.32 CBV.dial SSV @19:37:40.19 Allowing 130s for 25.0ml of remaining 450.0ml @19:37:40.20 Intake.select :open @19:37:42.36 Intake @16.2psia, vacuum from 9.7 to 13.7psi @19:37:42.38 SS.reconfigure SSinhale.with maxSpeed: 19 @19:37:42.42 SS.seek 25ml @19:39:16.22 Stroke completed -- waiting for pressure to equalize ... @19:39:34.45 Intake.select :close @19:39:34.60 SSV.dial :exhaust @19:39:35.45 Exhaust.select :open @19:39:35.56 SS.reconfigure SSexhale @19:39:35.61 SS.seek 0ml @19:39:45.64 Exhaust.select :close @19:39:45.75 SSV.dial CBV @19:39:45.77 CBV.dial SSV @19:39:46.64 Allowing 130s for 25.0ml of remaining 425.0ml @19:39:46.65 Intake.select :open @19:39:48.82 Intake @16.2psia, vacuum from 9.7 to 13.7psi @19:39:48.84 SS.reconfigure SSinhale.with maxSpeed: 19 @19:39:48.88 SS.seek 25ml @19:41:23.36 Stroke completed -- waiting for pressure to equalize ... @19:41:39.29 Intake.select :close @19:41:39.44 SSV.dial :exhaust @19:41:40.28 Exhaust.select :open @19:41:40.39 SS.reconfigure SSexhale @19:41:40.44 SS.seek 0ml @19:41:50.27 Exhaust.select :close @19:41:50.38 SSV.dial CBV @19:41:50.40 CBV.dial SSV @19:41:51.27 Allowing 130s for 25.0ml of remaining 400.0ml @19:41:51.28 Intake.select :open @19:41:53.44 Intake @16.3psia, vacuum from 9.8 to 13.8psi @19:41:53.45 SS.reconfigure SSinhale.with maxSpeed: 19 @19:41:53.50 SS.seek 25ml @19:43:27.42 Stroke completed -- waiting for pressure to equalize ... @19:43:45.11 Intake.select :close @19:43:45.26 SSV.dial :exhaust @19:43:46.12 Exhaust.select :open @19:43:46.23 SS.reconfigure SSexhale @19:43:46.28 SS.seek 0ml @19:43:56.01 Exhaust.select :close @19:43:56.12 SSV.dial CBV @19:43:56.14 CBV.dial SSV @19:43:57.01 Allowing 130s for 25.0ml of remaining 375.0ml @19:43:57.02 Intake.select :open @19:43:59.18 Intake @16.3psia, vacuum from 9.8 to 13.8psi @19:43:59.20 SS.reconfigure SSinhale.with maxSpeed: 19 @19:43:59.25 SS.seek 25ml @19:45:01.92 Vacuum too high -- waiting up to 40s @19:45:10.14 Intake @16.1psia, vacuum from 9.6 to 13.6psi @19:45:10.16 SS.reconfigure SSinhale.with maxSpeed: 17 @19:45:10.20 SS.seek 25ml @19:45:45.58 Stroke completed -- waiting for pressure to equalize ... @19:45:57.24 Intake.select :close @19:45:57.39 SSV.dial :exhaust @19:45:58.25 Exhaust.select :open @19:45:58.36 SS.reconfigure SSexhale @19:45:58.41 SS.seek 0ml @19:46:08.22 Exhaust.select :close @19:46:08.33 SSV.dial CBV @19:46:08.35 CBV.dial SSV @19:46:09.20 Allowing 130s for 25.0ml of remaining 350.0ml @19:46:09.21 Intake.select :open @19:46:11.37 Intake @16.3psia, vacuum from 9.8 to 13.8psi @19:46:11.39 SS.reconfigure SSinhale.with maxSpeed: 17 @19:46:11.43 SS.seek 25ml @19:47:56.95 Stroke completed -- waiting for pressure to equalize ... @19:48:10.35 Intake.select :close @19:48:10.50 SSV.dial :exhaust @19:48:11.34 Exhaust.select :open @19:48:11.45 SS.reconfigure SSexhale @19:48:11.50 SS.seek 0ml @19:48:21.22 Exhaust.select :close @19:48:21.33 SSV.dial CBV @19:48:21.35 CBV.dial SSV @19:48:22.20 Allowing 130s for 25.0ml of remaining 325.0ml @19:48:22.21 Intake.select :open @19:48:24.37 Intake @16.3psia, vacuum from 9.8 to 13.8psi @19:48:24.39 SS.reconfigure SSinhale.with maxSpeed: 17 @19:48:24.43 SS.seek 25ml @19:50:09.75 Stroke completed -- waiting for pressure to equalize ... @19:50:24.38 Intake.select :close @19:50:24.53 SSV.dial :exhaust @19:50:25.39 Exhaust.select :open @19:50:25.50 SS.reconfigure SSexhale @19:50:25.55 SS.seek 0ml @19:50:35.47 Exhaust.select :close @19:50:35.58 SSV.dial CBV @19:50:35.60 CBV.dial SSV @19:50:36.47 Allowing 130s for 25.0ml of remaining 300.0ml @19:50:36.48 Intake.select :open @19:50:38.65 Intake @16.3psia, vacuum from 9.8 to 13.8psi @19:50:38.67 SS.reconfigure SSinhale.with maxSpeed: 17 @19:50:38.71 SS.seek 25ml @19:52:24.07 Stroke completed -- waiting for pressure to equalize ... @19:52:36.94 Intake.select :close @19:52:37.09 SSV.dial :exhaust @19:52:37.95 Exhaust.select :open @19:52:38.06 SS.reconfigure SSexhale @19:52:38.11 SS.seek 0ml @19:52:47.93 Exhaust.select :close @19:52:48.04 SSV.dial CBV @19:52:48.06 CBV.dial SSV @19:52:48.92 Allowing 130s for 25.0ml of remaining 275.0ml @19:52:48.93 Intake.select :open @19:52:51.09 Intake @16.3psia, vacuum from 9.8 to 13.8psi @19:52:51.11 SS.reconfigure SSinhale.with maxSpeed: 17 @19:52:51.15 SS.seek 25ml @19:54:36.48 Stroke completed -- waiting for pressure to equalize ... @19:54:50.49 Intake.select :close @19:54:50.64 SSV.dial :exhaust @19:54:51.48 Exhaust.select :open @19:54:51.59 SS.reconfigure SSexhale @19:54:51.64 SS.seek 0ml @19:55:01.32 Exhaust.select :close @19:55:01.43 SSV.dial CBV @19:55:01.45 CBV.dial SSV @19:55:02.32 Allowing 130s for 25.0ml of remaining 250.0ml @19:55:02.33 Intake.select :open @19:55:04.49 Intake @16.3psia, vacuum from 9.8 to 13.8psi @19:55:04.51 SS.reconfigure SSinhale.with maxSpeed: 17 @19:55:04.55 SS.seek 25ml @19:56:49.81 Stroke completed -- waiting for pressure to equalize ... @19:57:04.52 Intake.select :close @19:57:04.67 SSV.dial :exhaust @19:57:05.97 Exhaust.select :open @19:57:06.17 SS.reconfigure SSexhale @19:57:06.22 SS.seek 0ml @19:57:15.99 Exhaust.select :close @19:57:16.10 SSV.dial CBV @19:57:16.12 CBV.dial SSV @19:57:16.99 Allowing 130s for 25.0ml of remaining 225.0ml @19:57:17.00 Intake.select :open @19:57:19.17 Intake @16.3psia, vacuum from 9.8 to 13.8psi @19:57:19.19 SS.reconfigure SSinhale.with maxSpeed: 17 @19:57:19.23 SS.seek 25ml @19:59:04.56 Stroke completed -- waiting for pressure to equalize ... @19:59:19.27 Intake.select :close @19:59:19.42 SSV.dial :exhaust @19:59:20.28 Exhaust.select :open @19:59:20.39 SS.reconfigure SSexhale @19:59:20.44 SS.seek 0ml @19:59:30.24 Exhaust.select :close @19:59:30.35 SSV.dial CBV @19:59:30.37 CBV.dial SSV @19:59:31.23 Allowing 130s for 25.0ml of remaining 200.0ml @19:59:31.24 Intake.select :open @19:59:33.40 Intake @16.3psia, vacuum from 9.8 to 13.8psi @19:59:33.42 SS.reconfigure SSinhale.with maxSpeed: 17 @19:59:33.46 SS.seek 25ml @20:01:18.77 Stroke completed -- waiting for pressure to equalize ... @20:01:34.00 Intake.select :close @20:01:34.15 SSV.dial :exhaust @20:01:35.00 Exhaust.select :open @20:01:35.11 SS.reconfigure SSexhale @20:01:35.16 SS.seek 0ml @20:01:44.81 Exhaust.select :close @20:01:44.92 SSV.dial CBV @20:01:44.94 CBV.dial SSV @20:01:45.79 Allowing 130s for 25.0ml of remaining 175.0ml @20:01:45.80 Intake.select :open @20:01:47.97 Intake @16.3psia, vacuum from 9.8 to 13.8psi @20:01:47.99 SS.reconfigure SSinhale.with maxSpeed: 17 @20:01:48.03 SS.seek 25ml @20:03:27.29 Vacuum too high -- waiting up to 40s @20:03:55.82 SS.reconfigure SSconfig @20:03:55.86 SS.hold @20:03:55.98 Intake.select :close @20:03:56.13 ShallowSampler::Clogged in WCR -- progress too slow after 1348.7ml @20:03:56.14 SSV.dial :exhaust @20:03:56.99 Exhaust.select :open @20:03:57.10 SS.reconfigure SSexhale @20:03:57.15 SS.seek 0ml @20:04:06.46 Exhaust.select :close @20:04:06.57 Sampled 1348.7ml @20:04:06.59 Drying puck before fixing for 4:00 @20:04:06.60 CBV.dial :waste2b @20:04:07.48 delay 1:00 @20:04:09.58 Gate.power :sampler,:OFF @20:05:07.50 CSV.dial :air @20:05:08.02 CS.reconfigure CSfast @20:05:08.06 CS.seek 10ml @20:05:34.96 Pumping wcr puck from tube 2 up to 55psi... @20:05:34.97 CBV.dial SSV @20:05:36.40 CBV.dial between CSV and waste2b @20:05:38.02 CSV.dial between air and CTV @20:05:38.39 CS.seek 6.881ml @20:05:47.46 CTV.dial CSV,avoiding: :air @20:05:47.49 CSV.dial CTV @20:05:48.39 CS.reconfigure CSpressurize @20:05:48.43 CS.seek 0ml @20:06:06.74 CSV.dial :air @20:06:07.27 CS.reconfigure CSfast @20:06:07.31 CS.seek 10ml @20:06:33.99 CSV.dial between air and CTV @20:06:34.35 CS.seek 2.573ml @20:06:54.56 CTV.dial CSV,avoiding: :air @20:06:54.59 CSV.dial CTV @20:06:54.93 CS.reconfigure CSpressurize @20:06:54.97 CS.seek 0ml @20:06:59.30 CBV.dial :waste2b @20:06:59.66 CS.reconfigure CSconfig @20:06:59.71 CS.seek :empty @20:07:03.68 delay 1:00 @20:08:03.70 CTV.dial :air,avoiding: :intake @20:08:05.22 Fix #1 of 2 (with rnal) for 2:00 @20:08:05.23 CSV.dial CSR @20:08:05.79 Pulling 2ml of CSR.rnal @20:08:05.80 CSR.select :rnal @20:08:05.91 CS.seek 2ml @20:08:12.06 delay 5 seconds @20:08:17.08 CSR.select :air @20:08:17.20 CS.reconfigure CSfast @20:08:17.25 CS.seek 10ml @20:08:38.84 Pumping wcr puck from tube 2 up to 55psi... @20:08:38.85 CBV.dial SSV @20:08:40.28 CBV.dial between CSV and waste2b @20:08:41.40 CSV.dial between air and CTV @20:08:42.10 CS.seek 6.858ml @20:08:51.37 CTV.dial CSV,avoiding: :air @20:08:51.40 CSV.dial CTV @20:08:52.98 CS.reconfigure CSpressurize @20:08:53.02 CS.seek 0ml @20:09:11.25 CSV.dial :air @20:09:11.76 CS.reconfigure CSfast @20:09:11.81 CS.seek 10ml @20:09:38.31 CSV.dial between air and CTV @20:09:39.18 CS.seek 2.033ml @20:10:00.59 CTV.dial CSV,avoiding: :air @20:10:00.62 CSV.dial CTV @20:10:00.94 CS.reconfigure CSpressurize @20:10:00.98 CS.seek 0ml @20:10:01.89 delay 2:00 @20:12:01.91 CBV.dial :waste2b @20:12:02.26 CS.reconfigure CSconfig @20:12:02.31 CS.seek :empty @20:12:08.28 delay 1:00 @20:13:08.30 CTV.dial :air,avoiding: :intake @20:13:09.83 Fix #2 of 2 (with rnal) for 2:00 @20:13:09.85 CSV.dial CSR @20:13:10.40 Pulling 2ml of CSR.rnal @20:13:10.41 CSR.select :rnal @20:13:10.52 CS.seek 2ml @20:13:16.69 delay 5 seconds @20:13:21.71 CSR.select :air @20:13:21.83 CS.reconfigure CSfast @20:13:21.88 CS.seek 10ml @20:13:43.31 Pumping wcr puck from tube 2 up to 55psi... @20:13:43.32 CBV.dial SSV @20:13:44.75 CBV.dial between CSV and waste2b @20:13:45.85 CSV.dial between air and CTV @20:13:46.56 CS.seek 6.858ml @20:13:55.56 CTV.dial CSV,avoiding: :air @20:13:55.59 CSV.dial CTV @20:13:57.20 CS.reconfigure CSpressurize @20:13:57.24 CS.seek 0ml @20:14:15.62 CSV.dial :air @20:14:16.15 CS.reconfigure CSfast @20:14:16.20 CS.seek 10ml @20:14:42.84 CSV.dial between air and CTV @20:14:43.20 CS.seek 1.993ml @20:15:04.69 CTV.dial CSV,avoiding: :air @20:15:04.72 CSV.dial CTV @20:15:05.06 CS.reconfigure CSpressurize @20:15:05.10 CS.seek 0ml @20:15:05.90 delay 2:00 @20:17:05.92 CBV.dial :waste2b @20:17:06.30 CS.reconfigure CSconfig @20:17:06.35 CS.seek :empty @20:17:12.28 delay 1:00 @20:18:12.30 CTV.dial :air,avoiding: :intake @20:18:13.87 Storing wcr puck in Collection Clamp to tube 1 @20:18:14.04 SC.reconfigure SCtight @20:18:14.08 SC.seek 1 @20:18:16.25 SC.reconfigure SCloose @20:18:16.45 Elbow.reconfigure TightElbow @20:18:16.50 Elbow.seek 1 @20:18:18.40 Forearm.reconfigure TightForearm @20:18:18.45 Forearm.seek 1 + 700 counts @20:18:19.51 SE.reconfigure SEpick @20:18:19.58 SE.seek beamBreak @20:18:22.69 SE.configure SEconfig @20:18:22.84 Elbow.reconfigure LimpElbow @20:18:22.89 Forearm.reconfigure LooseForearm @20:18:22.93 Forearm.seek 1 @20:18:23.23 Elbow.hold @20:18:23.34 Elbow.reconfigure TightElbow @20:18:23.44 SE.seek 1.07 Pucks below Top Plate @20:18:23.94 Forearm.force -20 @20:18:23.98 Forearm.seek 1 + 346 counts @20:18:24.28 Forearm.reconfigure TightForearm @20:18:24.33 Forearm.seek 1 + 700 counts @20:18:24.63 SE.seek 2.07 Pucks below Top Plate @20:18:25.34 Forearm.reconfigure LooseForearm @20:18:25.39 Forearm.seek :retract @20:18:26.37 Elbow.seek CC @20:18:27.99 Elbow.reconfigure LooseElbow @20:18:28.08 Forearm.seek CC @20:18:29.15 Hand.close @20:18:29.87 CC.seek :open @20:18:34.39 Forearm.seek :retract @20:18:35.43 Elbow.reconfigure TightElbow @20:18:35.48 Elbow.seek 1 @20:18:37.89 Elbow.reconfigure LimpElbow @20:18:37.94 Forearm.seek 1 @20:18:38.99 Elbow.hold @20:18:39.06 Elbow.reconfigure TightElbow @20:18:39.10 Hand.open @20:18:39.99 Forearm.force -20 @20:18:40.03 Forearm.seek 1 + 344 counts @20:18:40.33 Forearm.reconfigure TightForearm @20:18:40.38 Forearm.seek 1 + 700 counts @20:18:40.80 SE.seek :down @20:18:41.93 Forearm.reconfigure LooseForearm @20:18:41.97 Forearm.seek :retract @20:18:43.44 SE.coast @20:18:43.47 SC.coast @20:18:43.51 Starting finalPurge @20:18:43.52 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:18:43.59 Hand.open @20:18:43.99 Elbow.seek FlushPuck::Garage @20:18:46.02 Elbow.reconfigure LooseElbow @20:18:46.11 Forearm.seek :garage @20:18:47.18 Hand.close @20:18:47.99 Forearm.seek :retract @20:18:49.01 Elbow.reconfigure TightElbow @20:18:49.05 Elbow.seek CC @20:18:51.97 Elbow.reconfigure LooseElbow @20:18:52.06 Forearm.reconfigure TightForearm @20:18:52.10 Forearm.seek CC @20:18:53.39 CC.seek :closed @20:18:57.74 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.36 @20:18:57.81 Hand.open @20:18:58.55 Forearm.reconfigure LooseForearm @20:18:58.59 Forearm.seek :retract @20:18:59.60 Flushing the Collection Valve::Manifold @20:18:59.74 CSV.dial CSR @20:19:00.30 Pulling 5ml of CSR.flush CSR.select :flush @20:19:00.43 CS.seek 5ml @20:19:14.19 delay 5 seconds @20:19:19.21 CSR.select :air @20:19:19.37 CS.reconfigure CSfast @20:19:19.41 CS.seek 7.5ml @20:19:26.94 CSV.dial :waste @20:19:29.07 CS.reconfigure CSconfig @20:19:29.12 CS.seek 0ml @20:19:49.22 Flushing lower manifold's waste1 port @20:19:49.34 CSV.dial :flush @20:19:49.87 CS.reconfigure CSfast @20:19:49.91 CS.seek 5ml @20:20:03.82 delay 5 seconds @20:20:08.84 CSV.dial :air @20:20:09.42 CS.seek 7ml @20:20:15.41 CSV.dial CTV @20:20:15.44
CTV.dial CSV @20:20:17.00 CBV.dial :waste1 @20:20:17.89 CS.seek 0ml @20:20:36.72 Flushing lower manifold's waste2b port @20:20:36.84 CSV.dial :flush @20:20:37.70 CS.seek 5ml @20:20:51.53 delay 5 seconds @20:20:56.55 CSV.dial :air @20:20:57.14 CS.seek 7ml @20:21:03.28 CSV.dial CTV @20:21:03.32
CTV.dial CSV @20:21:03.78 CBV.dial :waste2b @20:21:04.65 CS.seek 0ml @20:21:23.57 Flushing lower manifold's waste1 port @20:21:23.58 CSV.dial CSR @20:21:24.14 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @20:21:24.25 CS.reconfigure CSconfig @20:21:24.30 CS.seek 4ml @20:21:35.57 delay 5 seconds @20:21:40.59 CSR.select :air @20:21:40.71 CS.reconfigure CSfast @20:21:40.75 CS.seek 10ml @20:21:57.13 CSV.dial CTV @20:21:57.16
CTV.dial CSV @20:21:57.71 CBV.dial :waste1 @20:21:58.58 CS.seek 0ml @20:22:25.10 Flushing lower manifold's waste2b port @20:22:25.11 CSV.dial CSR @20:22:25.69 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @20:22:25.80 CS.reconfigure CSconfig @20:22:25.85 CS.seek 4ml @20:22:37.00 delay 5 seconds @20:22:42.02 CSR.select :air @20:22:42.14 CS.reconfigure CSfast @20:22:42.18 CS.seek 10ml @20:22:58.38 CSV.dial CTV @20:22:58.41
CTV.dial CSV @20:22:58.94 CBV.dial :waste2b @20:22:59.81 CS.seek 0ml @20:23:26.13 Killing upper manifold @20:23:26.14 CSV.dial CSR @20:23:26.70 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @20:23:26.81 CS.reconfigure CSconfig @20:23:26.86 CS.seek 4ml @20:23:38.04 delay 5 seconds @20:23:43.06 CSR.select :air @20:23:43.18 CS.reconfigure CSfast @20:23:43.23 CS.seek 10ml @20:24:00.08 CSV.dial CBV @20:24:00.11
CBV.dial CSV @20:24:01.35 CTV.dial :air @20:24:02.87 CS.seek 0ml @20:24:29.44 delay 7 seconds @20:24:36.47 CS.seek 3ml @20:24:45.26 CSV.dial :waste @20:24:45.77 CS.seek 0ml @20:24:54.38 Flushing upper manifold @20:24:54.50 CSV.dial :flush @20:24:55.12 CS.seek 5ml @20:25:08.79 delay 5 seconds @20:25:13.81 CSV.dial :air @20:25:14.39 CS.seek 7ml @20:25:20.54 CSV.dial CBV @20:25:20.57
CBV.dial CSV @20:25:21.76 CTV.dial :air @20:25:21.78 CS.seek 0ml @20:25:40.57 delay 7 seconds @20:25:47.60 CS.seek 3ml @20:25:56.26 CSV.dial :waste @20:25:56.78 CS.seek 0ml @20:26:05.43 CSV.dial :flush @20:26:05.94 CS.seek 3ml @20:26:14.66 delay 5 seconds @20:26:19.68 CSV.dial :air @20:26:20.25 CS.seek 5ml @20:26:26.26 CSV.dial :air @20:26:26.28 CS.seek 0ml @20:26:39.94 delay 7 seconds @20:26:46.97 CS.seek 3ml @20:26:55.60 CSV.dial :waste @20:26:56.45 CS.seek 0ml @20:27:04.98 Flushing lower manifold's waste1 port @20:27:05.10 CSV.dial :flush @20:27:05.63 CS.seek 5ml @20:27:19.35 delay 5 seconds @20:27:24.37 CSV.dial :air @20:27:24.93 CS.seek 7ml @20:27:31.01 CSV.dial CTV @20:27:31.03
CTV.dial CSV @20:27:32.62 CBV.dial :waste1 @20:27:33.14 CS.seek 0ml @20:27:52.04 Flushing lower manifold's waste2b port @20:27:52.16 CSV.dial :flush @20:27:53.03 CS.seek 5ml @20:28:06.76 delay 5 seconds @20:28:11.78 CSV.dial :air @20:28:12.36 CS.seek 7ml @20:28:18.54 CSV.dial CTV @20:28:18.56
CTV.dial CSV @20:28:19.68 CBV.dial :waste2b @20:28:20.55 CS.seek 0ml @20:28:39.23 Garaging FlushPuck in Collection Clamp @20:28:39.30 Elbow.reconfigure TightElbow @20:28:39.35 Elbow.seek CC @20:28:39.58 Elbow.reconfigure LooseElbow @20:28:39.67 Forearm.seek CC @20:28:40.74 Hand.close @20:28:41.51 CC.seek :open @20:28:45.88 Forearm.seek :retract @20:28:46.94 Elbow.reconfigure TightElbow @20:28:46.99 Elbow.seek :garage @20:28:50.11 Elbow.reconfigure LooseElbow @20:28:50.20 Forearm.reconfigure TightForearm @20:28:50.24 Forearm.seek :garage @20:28:51.39 Hand.open @20:28:52.19 Forearm.reconfigure LooseForearm @20:28:52.23 Forearm.seek :retract @20:28:53.27 Starting initialPurge @20:28:53.28 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @20:28:53.34 Hand.open @20:28:53.69 Elbow.reconfigure TightElbow @20:28:53.73 Elbow.seek FlushPuck::Garage @20:28:53.97 Elbow.reconfigure LooseElbow @20:28:54.06 Forearm.seek :garage @20:28:55.08 Hand.close @20:28:55.99 Forearm.seek :retract @20:28:57.01 Elbow.reconfigure TightElbow @20:28:57.05 Elbow.seek CC @20:28:59.91 Elbow.reconfigure LooseElbow @20:29:00.00 Forearm.reconfigure TightForearm @20:29:00.04 Forearm.seek CC @20:29:01.32 CC.seek :closed @20:29:05.52 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.37 @20:29:05.60 Hand.open @20:29:06.44 Forearm.reconfigure LooseForearm @20:29:06.48 Forearm.seek :retract @20:29:07.47 Flushing the Collection Valve::Manifold @20:29:07.59 CSV.dial CSR @20:29:08.27 Pulling 5ml of CSR.flush CSR.select :flush @20:29:08.38 CS.reconfigure CSconfig @20:29:08.43 CS.seek 5ml @20:29:22.13 delay 5 seconds @20:29:27.15 CSR.select :air @20:29:27.31 CS.reconfigure CSfast @20:29:27.36 CS.seek 7.5ml @20:29:34.70 CSV.dial :waste @20:29:36.21 CS.reconfigure CSconfig @20:29:36.26 CS.seek 0ml @20:29:56.41 Purging external Intake @20:29:57.05 CSV.dial :flush @20:29:57.58 CS.reconfigure CSfast @20:29:57.62 CS.seek 10ml @20:30:24.10 delay 5 seconds @20:30:29.12 CSV.dial CBV @20:30:29.16
CBV.dial CSV @20:30:30.07 CTV.dial :intake @20:30:30.92 Intake.select :open @20:30:31.03 CS.seek 0ml @20:30:58.76 Intake.select :close @20:30:58.86 CTV.dial :air @20:30:59.71 Flushing upper manifold @20:30:59.83 CSV.dial :flush @20:31:00.68 CS.seek 5ml @20:31:14.35 delay 5 seconds @20:31:19.37 CSV.dial :air @20:31:20.05 CS.seek 7ml @20:31:26.17 CSV.dial CBV @20:31:26.20
CBV.dial CSV @20:31:27.37 CTV.dial :air @20:31:27.39 CS.seek 0ml @20:31:45.88 delay 7 seconds @20:31:52.91 CS.seek 3ml @20:32:01.45 CSV.dial :waste @20:32:01.97 CS.seek 0ml @20:32:10.59 CSV.dial :flush @20:32:11.10 CS.seek 3ml @20:32:19.63 delay 5 seconds @20:32:24.65 CSV.dial :air @20:32:25.20 CS.seek 5ml @20:32:31.20 CSV.dial :air @20:32:31.22 CS.seek 0ml @20:32:44.82 delay 7 seconds @20:32:51.85 CS.seek 3ml @20:33:00.32 CSV.dial :waste @20:33:01.18 CS.seek 0ml @20:33:09.67 Garaging FlushPuck in Collection Clamp @20:33:09.74 Elbow.reconfigure TightElbow @20:33:09.78 Elbow.seek CC @20:33:09.99 Elbow.reconfigure LooseElbow @20:33:10.07 Forearm.seek CC @20:33:11.12 Hand.close @20:33:11.88 CC.seek :open @20:33:16.25 Forearm.seek :retract @20:33:17.31 Elbow.reconfigure TightElbow @20:33:17.35 Elbow.seek :garage @20:33:20.55 Elbow.reconfigure LooseElbow @20:33:20.63 Forearm.reconfigure TightForearm @20:33:20.67 Forearm.seek :garage @20:33:21.84 Hand.open @20:33:22.69 Forearm.reconfigure LooseForearm @20:33:22.73 Forearm.seek :retract @20:33:23.79 Completed [mbaPhase] begun 16:24:37 @20:33:23.80 Extending 190517totalsampletest until completion of MFBPCR @20:34:09.41 PCR.off @20:34:11.92 PCR for IPc completed -- closed /var/log/esp/IPc19may1718h1000ml.pcr @20:34:11.98 Cleaning PCR @20:34:11.99 AFV.select :carrierSolution @20:34:12.09 delay 2 seconds @20:34:14.12 AS.reconfigure ASconfig @20:34:14.17 AS.seek 0.5ml @20:34:24.50 delay 1 second @20:34:25.52 AFV.select :holdingCoil @20:34:25.62 ASV.dial :waste1 @20:34:26.02 ATV.dial :air3 @20:34:27.33 AS.reconfigure ASslow1 @20:34:27.37 AS.seek 0.51ml @20:34:29.68 delay 2 seconds @20:34:31.70 ATV.dial :bleach @20:34:32.33 AS.seek 0.81ml @20:35:32.91 delay 3 seconds @20:35:35.93 ATV.dial :air3 @20:35:36.49 delay 3 seconds @20:35:39.56 AS.seek 0.835ml @20:35:44.86 delay 3 seconds @20:35:47.88 ATV.dial :bleach @20:35:48.50 AS.seek 0.82ml @20:35:51.83 delay 3 seconds @20:35:54.85 ARV.dial :waste @20:35:55.46 delay 3 seconds @20:35:58.48 ATV.dial :ARV @20:35:59.39 AS.reconfigure ASconfig @20:35:59.43 AS.seek 0.47ml @20:36:19.85 AFV.select :carrierSolution @20:36:19.95 delay 2 seconds @20:36:21.98 AS.seek 1ml @20:36:52.74 delay 1 second @20:36:53.76 AFV.select :holdingCoil @20:36:53.86 delay 1 second @20:36:54.89 AS.reconfigure ASfast @20:36:54.93 AS.seek 0ml @20:37:22.28 AFV.select :carrierSolution @20:37:22.38 delay 2 seconds @20:37:24.41 AS.reconfigure ASconfig @20:37:24.46 AS.seek 1ml @20:38:22.19 delay 1 second @20:38:23.21 AFV.select :holdingCoil @20:38:23.31 delay 1 second @20:38:24.34 AS.reconfigure ASfast @20:38:24.39 AS.seek 0ml @20:38:51.74 AFV.select :carrierSolution @20:38:51.84 delay 2 seconds @20:38:53.87 AS.reconfigure ASconfig @20:38:53.91 AS.seek 1ml @20:39:51.67 delay 1 second @20:39:52.69 AFV.select :holdingCoil @20:39:52.79 delay 1 second @20:39:53.82 AS.reconfigure ASfast @20:39:53.86 AS.seek 0ml @20:40:21.19 ARV.dial between carrier and air1 @20:40:21.67 ATV.dial :air3 @20:40:22.98 AS.reconfigure ASslow1 @20:40:23.02 AS.seek 0.01ml @20:40:25.27 delay 2 seconds @20:40:27.29 ATV.dial :bleach @20:40:27.91 AS.seek 0.81ml @20:43:09.05 delay 3 seconds @20:43:12.07 ATV.dial :air3 @20:43:12.61 delay 3 seconds @20:43:15.68 AS.seek 0.835ml @20:43:21.00 delay 3 seconds @20:43:24.02 ATV.dial :bleach @20:43:25.11 AS.seek 0.82ml @20:43:28.41 delay 3 seconds @20:43:31.43 ATV.dial PCR @20:43:31.83 AS.reconfigure ASconfig @20:43:31.88 AS.seek :empty @20:44:19.35 PCR.program :bleachProfile @20:44:19.40 PCR program should complete in < 2:45 @20:44:19.41 PCR.run 10:00 @20:47:15.16 PCR.off @20:47:15.19 AFV.select :carrierSolution @20:47:15.29 delay 2 seconds @20:47:17.32 AS.seek 1ml @20:48:15.14 delay 1 second @20:48:16.16 AFV.select :holdingCoil @20:48:16.26 delay 1 second @20:48:17.29 AS.reconfigure ASfast @20:48:17.34 AS.seek 0ml @20:48:44.67 delay 3 seconds @20:48:47.69 AFV.select :carrierSolution @20:48:47.79 delay 2 seconds @20:48:49.82 AS.reconfigure ASconfig @20:48:49.88 AS.seek 1ml @20:49:47.61 delay 1 second @20:49:48.63 AFV.select :holdingCoil @20:49:48.73 delay 1 second @20:49:49.76 AS.reconfigure ASfast @20:49:49.81 AS.seek 0ml @20:50:17.16 delay 3 seconds @20:50:20.18 AFV.select :carrierSolution @20:50:20.28 delay 2 seconds @20:50:22.31 AS.reconfigure ASconfig @20:50:22.36 AS.seek 1ml @20:51:20.14 delay 1 second @20:51:21.16 AFV.select :holdingCoil @20:51:21.26 delay 1 second @20:51:22.29 AS.reconfigure ASfast @20:51:22.34 AS.seek 0ml @20:51:49.67 delay 3 seconds @20:51:52.69 AFV.select :carrierSolution @20:51:52.79 delay 2 seconds @20:51:54.82 AS.reconfigure ASconfig @20:51:54.92 AS.seek 1ml @20:52:52.66 delay 1 second @20:52:53.68 AFV.select :holdingCoil @20:52:53.78 delay 1 second @20:52:54.81 AS.reconfigure ASfast @20:52:54.86 AS.seek 0ml @20:53:22.22 delay 3 seconds @20:53:25.24 AFV.select :carrierSolution @20:53:25.34 delay 2 seconds @20:53:27.37 AS.reconfigure ASconfig @20:53:27.41 AS.seek 1ml @20:54:25.17 delay 1 second @20:54:26.19 AFV.select :holdingCoil @20:54:26.29 delay 1 second @20:54:27.32 AS.reconfigure ASnorm @20:54:27.36 AS.seek 0ml @20:55:18.12 Setting up PCR for Pp -- writing /var/log/esp/Pp19may1720h1000ml.pcr @20:55:18.13 Building PCR Slug from ASV with MM1 mix, PP2 primer @20:55:18.15 Priming with carrier ATV.dial ARV @20:55:18.78 ARV.dial :carrier @20:55:19.11 AS.seek 0.5ml @20:55:44.64 AFV.select :carrierSolution @20:55:44.74 delay 2 seconds @20:55:46.77 AS.reconfigure ASconfig @20:55:46.82 AS.seek 1ml @20:56:15.83 delay 1 second @20:56:16.85 AFV.select :holdingCoil @20:56:16.96 delay 1 second @20:56:17.97 ATV.dial :waste @20:56:19.67 AS.reconfigure ASnorm @20:56:19.72 AS.seek :empty @20:57:10.46 AFV.select :carrierSolution @20:57:10.56 delay 2 seconds @20:57:12.59 AS.reconfigure ASconfig @20:57:12.64 AS.seek 0.5ml @20:57:41.74 delay 1 second @20:57:42.76 AFV.select :holdingCoil @20:57:42.87 Building Slug @20:57:42.88 ATV.dial :ARV @20:57:44.55 delay 2 seconds @20:57:46.57 ARV.dial :air1 @20:57:47.06 delay 2 seconds @20:57:49.13 AS.reconfigure ASslow2 @20:57:49.18 AS.seek 0.506ml @20:57:55.60 delay 2 seconds @20:57:57.62 ARV.dial :MM1 @20:57:58.24 delay 2 seconds @20:58:00.27 Adding MM1 @20:58:00.33 AS.seek 0.524ml @20:58:18.63 delay 25 seconds @20:58:43.65 ARV.dial :PP2 @20:58:44.05 Adding PP2 @20:58:44.06 delay 2 seconds @20:58:46.13 AS.seek 0.53ml @20:58:52.44 delay 10 seconds @20:59:02.47 ARV.dial :air2 @20:59:02.86 adding ARV air @20:59:03.37 delay 2 seconds @20:59:05.46 AS.seek 0.536ml @20:59:11.75 delay 5 seconds @20:59:16.77 ARV.dial :carrier @20:59:18.06 delay 7 seconds @20:59:25.15 AS.seek 0.594ml @21:00:23.58 delay 5 seconds @21:00:28.60 ATV.dial :ASV @21:00:29.00 delay 2 seconds @21:00:31.08 AS.seek 0.6ml @21:00:37.66 delay 4 seconds @21:00:41.68 ATV.dial ARV @21:00:42.22 delay 2 seconds @21:00:44.24 ARV.dial :carrier @21:00:44.25 delay 2 seconds @21:00:46.33 AS.seek 0.65ml @21:01:37.11 delay 4 seconds @21:01:41.13 ATV.dial PCR @21:01:41.75 ASV.dial :waste1 @21:01:41.76 ARV.dial :air1 @21:01:42.17 Positioning Slug by 0.324ml @21:01:42.25 AS.seek 0.326ml @21:07:08.49 delay 5 seconds @21:07:13.51 ATV.dial ARV @21:07:14.39 ASV.dial :PCRlock @21:07:14.78 delay 5 seconds @21:07:19.80 Running Pp Profile60C350 thermocycling @21:07:19.82 PCR.program Profile60C350 @21:07:19.94 Profile60C350 should complete in < 1:48:05 @21:07:19.95 PCR.run 2:20:06.25 @22:30:56.84 PCR.off @22:30:58.82 PCR for Pp completed -- closed /var/log/esp/Pp19may1720h1000ml.pcr @22:30:58.88 Cleaning PCR @22:30:58.89 AFV.select :carrierSolution @22:30:58.99 delay 2 seconds @22:31:01.02 AS.reconfigure ASconfig @22:31:01.07 AS.seek 0.5ml @22:31:11.34 delay 1 second @22:31:12.36 AFV.select :holdingCoil @22:31:12.46 ASV.dial :waste1 @22:31:12.86 ATV.dial :air3 @22:31:14.17 AS.reconfigure ASslow1 @22:31:14.21 AS.seek 0.51ml @22:31:16.50 delay 2 seconds @22:31:18.52 ATV.dial :bleach @22:31:19.15 AS.seek 0.81ml @22:32:19.74 delay 3 seconds @22:32:22.76 ATV.dial :air3 @22:32:23.33 delay 3 seconds @22:32:26.40 AS.seek 0.835ml @22:32:31.70 delay 3 seconds @22:32:34.72 ATV.dial :bleach @22:32:35.34 AS.seek 0.82ml @22:32:38.66 delay 3 seconds @22:32:41.68 ARV.dial :waste @22:32:42.30 delay 3 seconds @22:32:45.32 ATV.dial :ARV @22:32:46.22 AS.reconfigure ASconfig @22:32:46.26 AS.seek 0.47ml @22:33:06.67 AFV.select :carrierSolution @22:33:06.77 delay 2 seconds @22:33:08.80 AS.seek 1ml @22:33:39.56 delay 1 second @22:33:40.58 AFV.select :holdingCoil @22:33:40.68 delay 1 second @22:33:41.71 AS.reconfigure ASfast @22:33:41.76 AS.seek 0ml @22:34:09.06 AFV.select :carrierSolution @22:34:09.16 delay 2 seconds @22:34:11.19 AS.reconfigure ASconfig @22:34:11.23 AS.seek 1ml @22:35:09.02 delay 1 second @22:35:10.04 AFV.select :holdingCoil @22:35:10.14 delay 1 second @22:35:11.17 AS.reconfigure ASfast @22:35:11.21 AS.seek 0ml @22:35:38.56 AFV.select :carrierSolution @22:35:38.66 delay 2 seconds @22:35:40.69 AS.reconfigure ASconfig @22:35:40.73 AS.seek 1ml @22:36:38.49 delay 1 second @22:36:39.51 AFV.select :holdingCoil @22:36:39.61 delay 1 second @22:36:40.64 AS.reconfigure ASfast @22:36:40.69 AS.seek 0ml @22:37:08.03 ARV.dial between carrier and air1 @22:37:08.53 ATV.dial :air3 @22:37:09.84 AS.reconfigure ASslow1 @22:37:09.88 AS.seek 0.01ml @22:37:12.70 delay 2 seconds @22:37:14.72 ATV.dial :bleach @22:37:15.34 AS.seek 0.81ml @22:39:56.47 delay 3 seconds @22:39:59.49 ATV.dial :air3 @22:40:00.03 delay 3 seconds @22:40:03.10 AS.seek 0.835ml @22:40:08.39 delay 3 seconds @22:40:11.41 ATV.dial :bleach @22:40:12.03 AS.seek 0.82ml @22:40:15.34 delay 3 seconds @22:40:18.36 ATV.dial PCR @22:40:18.75 AS.reconfigure ASconfig @22:40:18.80 AS.seek :empty @22:41:06.25 PCR.program :bleachProfile @22:41:06.30 PCR program should complete in < 2:45 @22:41:06.31 PCR.run 10:00 @22:44:02.07 PCR.off @22:44:02.10 AFV.select :carrierSolution @22:44:02.20 delay 2 seconds @22:44:04.23 AS.seek 1ml @22:45:02.06 delay 1 second @22:45:03.08 AFV.select :holdingCoil @22:45:03.18 delay 1 second @22:45:04.21 AS.reconfigure ASfast @22:45:04.26 AS.seek 0ml @22:45:31.59 delay 3 seconds @22:45:34.61 AFV.select :carrierSolution @22:45:34.71 delay 2 seconds @22:45:36.74 AS.reconfigure ASconfig @22:45:36.79 AS.seek 1ml @22:46:34.52 delay 1 second @22:46:35.54 AFV.select :holdingCoil @22:46:35.64 delay 1 second @22:46:36.67 AS.reconfigure ASfast @22:46:36.72 AS.seek 0ml @22:47:04.06 delay 3 seconds @22:47:07.08 AFV.select :carrierSolution @22:47:07.18 delay 2 seconds @22:47:09.21 AS.reconfigure ASconfig @22:47:09.26 AS.seek 1ml @22:48:07.00 delay 1 second @22:48:08.02 AFV.select :holdingCoil @22:48:08.12 delay 1 second @22:48:09.15 AS.reconfigure ASfast @22:48:09.20 AS.seek 0ml @22:48:36.53 delay 3 seconds @22:48:39.55 AFV.select :carrierSolution @22:48:39.65 delay 2 seconds @22:48:41.68 AS.reconfigure ASconfig @22:48:41.72 AS.seek 1ml @22:49:39.50 delay 1 second @22:49:40.52 AFV.select :holdingCoil @22:49:40.62 delay 1 second @22:49:41.65 AS.reconfigure ASfast @22:49:41.70 AS.seek 0ml @22:50:09.03 delay 3 seconds @22:50:12.05 AFV.select :carrierSolution @22:50:12.15 delay 2 seconds @22:50:14.18 AS.reconfigure ASconfig @22:50:14.23 AS.seek 1ml @22:51:11.99 delay 1 second @22:51:13.01 AFV.select :holdingCoil @22:51:13.11 delay 1 second @22:51:14.14 AS.reconfigure ASnorm @22:51:14.18 AS.seek 0ml @22:52:04.93 Setting up PCR for Ss -- writing /var/log/esp/Ss19may1722h1000ml.pcr @22:52:04.94 Building PCR Slug from ASV with MM2 mix, PP4 primer @22:52:04.96 Priming with carrier ATV.dial ARV @22:52:05.58 ARV.dial :carrier @22:52:05.92 AS.seek 0.5ml @22:52:31.49 AFV.select :carrierSolution @22:52:31.59 delay 2 seconds @22:52:33.62 AS.reconfigure ASconfig @22:52:33.67 AS.seek 1ml @22:53:02.67 delay 1 second @22:53:03.69 AFV.select :holdingCoil @22:53:03.79 delay 1 second @22:53:04.81 ATV.dial :waste @22:53:06.52 AS.reconfigure ASnorm @22:53:06.57 AS.seek :empty @22:53:57.30 AFV.select :carrierSolution @22:53:57.41 delay 2 seconds @22:53:59.44 AS.reconfigure ASconfig @22:53:59.49 AS.seek 0.5ml @22:54:28.58 delay 1 second @22:54:29.60 AFV.select :holdingCoil @22:54:29.71 Building Slug @22:54:29.72 ATV.dial :ARV @22:54:31.39 delay 2 seconds @22:54:33.41 ARV.dial :air1 @22:54:33.81 delay 2 seconds @22:54:35.88 AS.reconfigure ASslow2 @22:54:35.93 AS.seek 0.506ml @22:54:42.17 delay 2 seconds @22:54:44.19 ARV.dial :MM2 @22:54:45.67 delay 2 seconds @22:54:47.70 Adding MM2 @22:54:47.75 AS.seek 0.524ml @22:55:06.48 delay 25 seconds @22:55:31.50 ARV.dial :PP4 @22:55:31.89 Adding PP4 @22:55:31.90 delay 2 seconds @22:55:33.98 AS.seek 0.53ml @22:55:40.19 delay 10 seconds @22:55:50.22 ARV.dial :air3 @22:55:50.61 adding ARV air @22:55:50.62 delay 2 seconds @22:55:52.70 AS.seek 0.536ml @22:55:58.92 delay 5 seconds @22:56:03.94 ARV.dial :carrier @22:56:05.22 delay 7 seconds @22:56:12.31 AS.seek 0.594ml @22:57:11.00 delay 5 seconds @22:57:16.02 ATV.dial :ASV @22:57:16.42 delay 2 seconds @22:57:18.49 AS.seek 0.6ml @22:57:25.09 delay 4 seconds @22:57:29.11 ATV.dial ARV @22:57:29.66 delay 2 seconds @22:57:31.68 ARV.dial :carrier @22:57:31.69 delay 2 seconds @22:57:33.76 AS.seek 0.65ml @22:58:24.49 delay 4 seconds @22:58:28.51 ATV.dial PCR @22:58:29.14 ASV.dial :waste1 @22:58:29.15 ARV.dial :air1 @22:58:29.54 Positioning Slug by 0.324ml @22:58:29.62 AS.seek 0.326ml @23:03:55.50 delay 5 seconds @23:04:00.52 ATV.dial ARV @23:04:01.28 ASV.dial :PCRlock @23:04:01.67 delay 5 seconds @23:04:06.69 Running Ss Profile60C350 thermocycling @23:04:06.71 PCR.program Profile60C350 @23:04:06.83 Profile60C350 should complete in < 1:48:05 @23:04:06.84 PCR.run 2:20:06.25 @00:27:40.91CEST18-May-19 PCR.off @00:27:42.90 PCR for Ss completed -- closed /var/log/esp/Ss19may1722h1000ml.pcr @00:27:42.96 Cleaning PCR @00:27:42.97 AFV.select :carrierSolution @00:27:43.07 delay 2 seconds @00:27:45.10 AS.reconfigure ASconfig @00:27:45.15 AS.seek 0.5ml @00:27:55.45 delay 1 second @00:27:56.47 AFV.select :holdingCoil @00:27:56.57 ASV.dial :waste1 @00:27:56.97 ATV.dial :air3 @00:27:58.28 AS.reconfigure ASslow1 @00:27:58.32 AS.seek 0.51ml @00:28:00.60 delay 2 seconds @00:28:02.62 ATV.dial :bleach @00:28:03.25 AS.seek 0.81ml @00:29:03.86 delay 3 seconds @00:29:06.88 ATV.dial :air3 @00:29:07.42 delay 3 seconds @00:29:10.49 AS.seek 0.835ml @00:29:15.81 delay 3 seconds @00:29:18.83 ATV.dial :bleach @00:29:19.45 AS.seek 0.82ml @00:29:22.78 delay 3 seconds @00:29:25.80 ARV.dial :waste @00:29:26.41 delay 3 seconds @00:29:29.43 ATV.dial :ARV @00:29:30.33 AS.reconfigure ASconfig @00:29:30.37 AS.seek 0.47ml @00:29:50.78 AFV.select :carrierSolution @00:29:50.88 delay 2 seconds @00:29:52.91 AS.seek 1ml @00:30:23.69 delay 1 second @00:30:24.71 AFV.select :holdingCoil @00:30:24.81 delay 1 second @00:30:25.84 AS.reconfigure ASfast @00:30:25.88 AS.seek 0ml @00:30:53.22 AFV.select :carrierSolution @00:30:53.32 delay 2 seconds @00:30:55.35 AS.reconfigure ASconfig @00:30:55.39 AS.seek 1ml @00:31:53.14 delay 1 second @00:31:54.16 AFV.select :holdingCoil @00:31:54.26 delay 1 second @00:31:55.29 AS.reconfigure ASfast @00:31:55.34 AS.seek 0ml @00:32:22.69 AFV.select :carrierSolution @00:32:22.79 delay 2 seconds @00:32:24.82 AS.reconfigure ASconfig @00:32:24.87 AS.seek 1ml @00:33:22.61 delay 1 second @00:33:23.63 AFV.select :holdingCoil @00:33:23.73 delay 1 second @00:33:24.76 AS.reconfigure ASfast @00:33:24.81 AS.seek 0ml @00:33:52.14 ARV.dial between carrier and air1 @00:33:53.11 ATV.dial :air3 @00:33:54.44 AS.reconfigure ASslow1 @00:33:54.48 AS.seek 0.01ml @00:33:56.78 delay 2 seconds @00:33:58.80 ATV.dial :bleach @00:33:59.40 AS.seek 0.81ml @00:36:40.53 delay 3 seconds @00:36:43.55 ATV.dial :air3 @00:36:44.09 delay 3 seconds @00:36:47.16 AS.seek 0.835ml @00:36:52.45 delay 3 seconds @00:36:55.47 ATV.dial :bleach @00:36:56.08 AS.seek 0.82ml @00:36:59.33 delay 3 seconds @00:37:02.35 ATV.dial PCR @00:37:02.75 AS.reconfigure ASconfig @00:37:02.80 AS.seek :empty @00:37:50.28 PCR.program :bleachProfile @00:37:50.33 PCR program should complete in < 2:45 @00:37:50.34 PCR.run 10:00 @00:40:46.09 PCR.off @00:40:46.12 AFV.select :carrierSolution @00:40:46.22 delay 2 seconds @00:40:48.25 AS.seek 1ml @00:41:46.05 delay 1 second @00:41:47.07 AFV.select :holdingCoil @00:41:47.17 delay 1 second @00:41:48.20 AS.reconfigure ASfast @00:41:48.25 AS.seek 0ml @00:42:15.59 delay 3 seconds @00:42:18.61 AFV.select :carrierSolution @00:42:18.71 delay 2 seconds @00:42:20.74 AS.reconfigure ASconfig @00:42:20.80 AS.seek 1ml @00:43:18.55 delay 1 second @00:43:19.57 AFV.select :holdingCoil @00:43:19.67 delay 1 second @00:43:20.70 AS.reconfigure ASfast @00:43:20.75 AS.seek 0ml @00:43:48.08 delay 3 seconds @00:43:51.10 AFV.select :carrierSolution @00:43:51.20 delay 2 seconds @00:43:53.23 AS.reconfigure ASconfig @00:43:53.28 AS.seek 1ml @00:44:51.03 delay 1 second @00:44:52.05 AFV.select :holdingCoil @00:44:52.15 delay 1 second @00:44:53.18 AS.reconfigure ASfast @00:44:53.23 AS.seek 0ml @00:45:20.56 delay 3 seconds @00:45:23.58 AFV.select :carrierSolution @00:45:23.68 delay 2 seconds @00:45:25.71 AS.reconfigure ASconfig @00:45:25.76 AS.seek 1ml @00:46:23.55 delay 1 second @00:46:24.57 AFV.select :holdingCoil @00:46:24.67 delay 1 second @00:46:25.70 AS.reconfigure ASfast @00:46:25.75 AS.seek 0ml @00:46:53.09 delay 3 seconds @00:46:56.11 AFV.select :carrierSolution @00:46:56.21 delay 2 seconds @00:46:58.24 AS.reconfigure ASconfig @00:46:58.30 AS.seek 1ml @00:47:56.05 delay 1 second @00:47:57.07 AFV.select :holdingCoil @00:47:57.17 delay 1 second @00:47:58.20 AS.reconfigure ASnorm @00:47:58.25 AS.seek 0ml @00:48:48.96 Setting up PCR for Mm -- writing /var/log/esp/Mm19may1800h1000ml.pcr @00:48:48.97 Building PCR Slug from ASV with MM2 mix, PP3 primer @00:48:48.99 Priming with carrier ATV.dial ARV @00:48:49.61 ARV.dial :carrier @00:48:49.95 AS.seek 0.5ml @00:49:15.55 AFV.select :carrierSolution @00:49:15.65 delay 2 seconds @00:49:17.68 AS.reconfigure ASconfig @00:49:17.73 AS.seek 1ml @00:49:46.73 delay 1 second @00:49:47.75 AFV.select :holdingCoil @00:49:47.85 delay 1 second @00:49:48.87 ATV.dial :waste @00:49:50.58 AS.reconfigure ASnorm @00:49:50.62 AS.seek :empty @00:50:41.34 AFV.select :carrierSolution @00:50:41.44 delay 2 seconds @00:50:43.47 AS.reconfigure ASconfig @00:50:43.52 AS.seek 0.5ml @00:51:12.63 delay 1 second @00:51:13.65 AFV.select :holdingCoil @00:51:13.76 Building Slug @00:51:13.77 ATV.dial :ARV @00:51:15.44 delay 2 seconds @00:51:17.46 ARV.dial :air1 @00:51:17.84 delay 2 seconds @00:51:19.91 AS.reconfigure ASslow2 @00:51:19.96 AS.seek 0.506ml @00:51:26.33 delay 2 seconds @00:51:28.85 ARV.dial :MM2 @00:51:30.36 delay 2 seconds @00:51:32.38 Adding MM2 @00:51:32.45 AS.seek 0.524ml @00:51:50.92 delay 25 seconds @00:52:15.94 ARV.dial :PP3 @00:52:16.33 Adding PP3 @00:52:16.34 delay 2 seconds @00:52:18.41 AS.seek 0.53ml @00:52:24.78 delay 10 seconds @00:52:34.81 ARV.dial :air2 @00:52:35.20 adding ARV air @00:52:35.21 delay 2 seconds @00:52:37.28 AS.seek 0.536ml @00:52:43.56 delay 5 seconds @00:52:48.58 ARV.dial :carrier @00:52:49.88 delay 7 seconds @00:52:56.97 AS.seek 0.594ml @00:53:55.56 delay 5 seconds @00:54:00.58 ATV.dial :ASV @00:54:00.98 delay 2 seconds @00:54:03.05 AS.seek 0.6ml @00:54:09.53 delay 4 seconds @00:54:13.55 ATV.dial ARV @00:54:14.09 delay 2 seconds @00:54:16.11 ARV.dial :carrier @00:54:16.12 delay 2 seconds @00:54:18.20 AS.seek 0.65ml @00:55:08.83 delay 4 seconds @00:55:12.85 ATV.dial PCR @00:55:13.47 ASV.dial :waste1 @00:55:13.48 ARV.dial :air1 @00:55:13.89 Positioning Slug by 0.324ml @00:55:13.97 AS.seek 0.326ml @01:00:40.00 delay 5 seconds @01:00:45.02 ATV.dial ARV @01:00:45.91 ASV.dial :PCRlock @01:00:46.30 delay 5 seconds @01:00:51.33 Running Mm Profile60C350 thermocycling @01:00:51.34 PCR.program Profile60C350 @01:00:51.46 Profile60C350 should complete in < 1:48:05 @01:00:51.47 PCR.run 2:20:06.25 @02:24:32.52 PCR.off @02:24:34.50 PCR for Mm completed -- closed /var/log/esp/Mm19may1800h1000ml.pcr @02:24:34.52 Full MFB Decontamination @02:24:34.53 Decon extraction column and decon AIV @02:24:34.55 SPE.hold 2:46:40,55C @02:24:34.58 ASV.dial :SPEdelivery @02:24:35.25 delay 20 seconds @02:24:55.28 AFV.select :carrierSolution @02:24:55.38 delay 2 seconds @02:24:57.41 AS.reconfigure ASconfig @02:24:57.45 AS.seek 0.5ml @02:25:07.75 delay 1 second @02:25:08.77 AFV.select :holdingCoil @02:25:08.87 ATV.dial :air1 @02:25:11.09 AS.reconfigure ASslow1 @02:25:11.14 AS.seek 0.52ml @02:25:15.45 ATV.dial :ASV @02:25:17.17 ASV.dial :SPErecovery @02:25:17.84 AS.seek 0.62ml @02:25:38.23 ATV.dial :waste @02:25:40.34 AS.reconfigure ASnorm @02:25:40.39 AS.seek :empty @02:26:12.00 AFV.select :holdingCoil @02:26:12.01 ATV.dial :waste @02:26:12.03 AS.reconfigure ASconfig @02:26:12.07 AS.seek 0ml @02:26:12.37 AFV.select :carrierSolution @02:26:12.47 delay 2 seconds @02:26:14.50 AS.seek 0.5ml @02:26:43.52 delay 1 second @02:26:44.54 AFV.select :holdingCoil @02:26:44.64 ATV.dial :air3 @02:26:45.39 AS.reconfigure ASslow1 @02:26:45.43 AS.seek 0.52ml @02:26:49.77 ATV.dial :air3 @02:26:49.84 AS.seek 0.53ml @02:26:52.19 delay 2 seconds @02:26:54.21 ATV.dial :bleach @02:26:54.68 AS.seek 0.71ml @02:27:31.16 delay 3 seconds @02:27:34.18 ATV.dial :air3 @02:27:34.78 delay 3 seconds @02:27:37.85 AS.seek 0.735ml @02:27:43.24 delay 3 seconds @02:27:46.26 ATV.dial :bleach @02:27:47.40 AS.seek 0.72ml @02:27:50.69 delay 3 seconds @02:27:53.71 ATV.dial ASV @02:27:54.83 ASV.dial :SPErecovery @02:27:54.91 AS.seek 0.365ml @02:29:06.55 ATV.dial :waste @02:29:08.37 AS.reconfigure ASnorm @02:29:08.42 AS.seek :empty @02:29:27.20 ATV.dial :air3 @02:29:27.90 AS.reconfigure ASslow1 @02:29:27.95 AS.seek 0.009ml @02:29:30.28 delay 2 seconds @02:29:32.30 ATV.dial :bleach @02:29:32.76 AS.seek 0.579ml @02:31:27.64 delay 3 seconds @02:31:30.66 ATV.dial :air3 @02:31:31.22 delay 3 seconds @02:31:34.29 AS.seek 0.604ml @02:31:39.61 delay 3 seconds @02:31:42.63 ATV.dial :bleach @02:31:43.25 AS.seek 0.589ml @02:31:46.53 delay 3 seconds @02:31:49.55 ATV.dial :ASV @02:31:50.61 ASV.dial :wasteR @02:31:50.99 AS.seek 0ml @02:33:49.73 delay 1:00 @02:34:49.75 ATV.dial ASV @02:34:49.76 AFV.select :carrierSolution @02:34:49.86 delay 2 seconds @02:34:51.89 AS.reconfigure ASconfig @02:34:51.94 AS.seek 1ml @02:35:49.70 delay 1 second @02:35:50.72 AFV.select :holdingCoil @02:35:50.82 delay 1 second @02:35:51.84 ASV.dial :wasteR @02:35:51.86 AS.reconfigure ASslow1 @02:35:51.91 AS.seek 0ml @02:39:13.30 delay 10 seconds @02:39:23.33 ATV.dial :air3 @02:39:24.86 AS.seek 0.01ml @02:39:27.16 delay 2 seconds @02:39:29.18 ATV.dial :bleach @02:39:29.81 AS.seek 0.58ml @02:41:24.72 delay 3 seconds @02:41:27.74 ATV.dial :air3 @02:41:28.30 delay 3 seconds @02:41:31.37 AS.seek 0.605ml @02:41:36.67 delay 3 seconds @02:41:39.69 ATV.dial :bleach @02:41:40.31 AS.seek 0.59ml @02:41:43.64 delay 3 seconds @02:41:46.66 ATV.dial ASV @02:41:47.72 ASV.dial :SPEdelivery @02:41:48.09 AS.seek 0ml @02:43:47.00 delay 1:00 @02:44:47.02 AFV.select :carrierSolution @02:44:47.12 delay 2 seconds @02:44:49.15 AS.reconfigure ASconfig @02:44:49.20 AS.seek 1ml @02:45:46.95 delay 1 second @02:45:47.97 AFV.select :holdingCoil @02:45:48.07 delay 1 second @02:45:49.09 ATV.dial ASV @02:45:49.10 ASV.dial :SPEdelivery @02:45:49.12 AS.reconfigure ASslow1 @02:45:49.17 AS.seek 0ml @02:49:10.55 delay 10 seconds @02:49:20.58 ATV.dial :air2 @02:49:21.43 AS.reconfigure ASslow0 @02:49:21.47 AS.seek 0.8ml @02:50:42.27 delay 3 seconds @02:50:45.29 ATV.dial :ASV @02:50:46.30 ASV.dial :SPEdelivery @02:50:46.32 AS.reconfigure ASslow1 @02:50:46.36 AS.seek 0.03ml @02:53:21.48 delay 20 seconds @02:53:41.51 ATV.dial :waste,via: :air3 @02:53:43.30 AS.reconfigure ASconfig @02:53:43.35 AS.seek :empty @02:53:45.42 SPE.coast @02:53:45.49 Removing Bleach from Mixing Coil @02:53:45.50 AFV.select :carrierSolution @02:53:45.59 delay 2 seconds @02:53:47.62 AS.seek 0.5ml @02:54:16.70 delay 1 second @02:54:17.72 AFV.select :holdingCoil @02:54:17.82 ATV.dial :ASV @02:54:19.58 ASV.dial :SPErecovery @02:54:20.22 AS.reconfigure ASslow1 @02:54:20.26 AS.seek 0.73ml @02:55:06.83 ASV.dial :wasteD @02:55:07.92 AS.reconfigure ASnorm @02:55:07.96 AS.seek 0ml @02:55:45.09 AFV.select :carrierSolution @02:55:45.19 delay 2 seconds @02:55:47.22 AS.reconfigure ASconfig @02:55:47.27 AS.seek 0.5ml @02:56:16.30 delay 1 second @02:56:17.32 AFV.select :holdingCoil @02:56:17.42 ATV.dial ASV @02:56:17.44 ASV.dial :SPErecovery @02:56:18.08 AS.reconfigure ASslow0 @02:56:18.12 AS.seek 0.3ml @02:56:38.53 ATV.dial :air2 @02:56:39.58 AS.reconfigure ASslow1 @02:56:39.63 AS.seek 0.32ml @02:56:43.88 ATV.dial ASV @02:56:44.92 AS.reconfigure ASslow0 @02:56:44.96 AS.seek 0.57ml @02:57:10.42 ASV.dial :wasteD @02:57:11.01 AS.reconfigure ASnorm @02:57:11.05 AS.seek 0ml @02:57:40.09 AFV.select :carrierSolution @02:57:40.19 delay 2 seconds @02:57:42.22 AS.reconfigure ASconfig @02:57:42.27 AS.seek 0.5ml @02:58:11.33 delay 1 second @02:58:12.35 AFV.select :holdingCoil @02:58:12.45 ATV.dial ASV @02:58:12.47 ASV.dial :SPErecovery @02:58:13.11 AS.reconfigure ASslow0 @02:58:13.15 AS.seek 0.3ml @02:58:33.56 ATV.dial :air2 @02:58:34.61 AS.reconfigure ASslow1 @02:58:34.65 AS.seek 0.32ml @02:58:39.03 ATV.dial ASV @02:58:40.09 AS.reconfigure ASslow0 @02:58:40.13 AS.seek 0.57ml @02:59:05.58 ASV.dial :wasteD @02:59:06.17 AS.reconfigure ASnorm @02:59:06.21 AS.seek 0ml @02:59:35.25 ATV.dial :air2 @02:59:36.24 AS.reconfigure ASslow0 @02:59:36.28 AS.seek 0.3ml @03:00:06.73 ATV.dial :ASV @03:00:07.73 ASV.dial :wasteD @03:00:07.75 AS.reconfigure ASconfig @03:00:07.79 AS.seek 0.05ml @03:00:22.47 ATV.dial :waste @03:00:24.37 AS.seek 0ml @03:00:27.54 ASV.dial :SPEdelivery @03:00:28.53 deconLysateINT @03:00:28.54 ATV.dial :air3 @03:00:29.23 AS.reconfigure ASslow1 @03:00:29.27 AS.seek 0.01ml @03:00:31.62 delay 2 seconds @03:00:33.64 ATV.dial :bleach @03:00:34.11 AS.seek 0.96ml @03:03:45.39 delay 3 seconds @03:03:48.41 ATV.dial :air3 @03:03:48.97 delay 3 seconds @03:03:52.04 AS.seek 0.985ml @03:03:57.32 delay 3 seconds @03:04:00.34 ATV.dial :bleach @03:04:00.97 AS.seek 0.97ml @03:04:04.28 delay 3 seconds @03:04:07.30 ATV.dial AIV @03:04:08.56 AIV.dial :coilToMFB @03:04:08.57 AWV.select :waste @03:04:08.58 AFV.select :carrierSolution @03:04:08.68 delay 2 seconds @03:04:10.71 AS.reconfigure ASconfig @03:04:10.76 AS.seek 1ml @03:04:12.78 delay 1 second @03:04:13.80 AFV.select :holdingCoil @03:04:13.90 delay 1 second @03:04:14.93 AS.reconfigure ASslow0 @03:04:14.97 AS.seek :empty @03:05:55.92 AFV.select :carrierSolution @03:05:56.02 delay 2 seconds @03:05:58.05 AS.reconfigure ASconfig @03:05:58.09 AS.seek 1ml @03:06:55.89 delay 1 second @03:06:56.91 AFV.select :holdingCoil @03:06:57.01 delay 1 second @03:06:58.04 AS.reconfigure ASslow0 @03:06:58.08 AS.seek :empty @03:08:39.06 AFV.select :carrierSolution @03:08:39.16 delay 2 seconds @03:08:41.19 AS.reconfigure ASconfig @03:08:41.24 AS.seek 1ml @03:09:39.03 delay 1 second @03:09:40.55 AFV.select :holdingCoil @03:09:40.66 delay 1 second @03:09:41.69 AS.reconfigure ASslow0 @03:09:41.73 AS.seek :empty @03:11:22.67 ATV.dial :air2 @03:11:23.34 AS.reconfigure ASslow1 @03:11:23.39 AS.seek 0.009ml @03:11:25.72 AS.reconfigure ASslow0 @03:11:25.77 AS.seek 1ml @03:13:05.70 ATV.dial AIV @03:13:06.48 AS.seek 0.02ml @03:14:45.31 ATV.dial :waste @03:14:46.99 AS.seek 0ml @03:14:49.31 AWV.select :air @03:14:49.46 Cleaning PCR @03:14:49.47 AFV.select :carrierSolution @03:14:49.56 delay 2 seconds @03:14:51.59 AS.reconfigure ASconfig @03:14:51.64 AS.seek 0.5ml @03:15:20.66 delay 1 second @03:15:21.68 AFV.select :holdingCoil @03:15:21.78 ASV.dial :waste1 @03:15:22.16 ATV.dial :air3 @03:15:22.84 AS.reconfigure ASslow1 @03:15:22.88 AS.seek 0.51ml @03:15:25.20 delay 2 seconds @03:15:27.22 ATV.dial :bleach @03:15:27.68 AS.seek 0.81ml @03:16:28.28 delay 3 seconds @03:16:31.30 ATV.dial :air3 @03:16:31.86 delay 3 seconds @03:16:34.93 AS.seek 0.835ml @03:16:40.23 delay 3 seconds @03:16:43.25 ATV.dial :bleach @03:16:43.86 AS.seek 0.82ml @03:16:47.16 delay 3 seconds @03:16:50.18 ARV.dial :waste @03:16:50.80 delay 3 seconds @03:16:53.82 ATV.dial :ARV @03:16:54.73 AS.reconfigure ASconfig @03:16:54.77 AS.seek 0.47ml @03:17:15.19 AFV.select :carrierSolution @03:17:15.29 delay 2 seconds @03:17:17.32 AS.seek 1ml @03:17:48.09 delay 1 second @03:17:49.11 AFV.select :holdingCoil @03:17:49.21 delay 1 second @03:17:50.24 AS.reconfigure ASfast @03:17:50.29 AS.seek 0ml @03:18:17.62 AFV.select :carrierSolution @03:18:17.72 delay 2 seconds @03:18:19.75 AS.reconfigure ASconfig @03:18:19.80 AS.seek 1ml @03:19:17.56 delay 1 second @03:19:18.58 AFV.select :holdingCoil @03:19:18.68 delay 1 second @03:19:19.71 AS.reconfigure ASfast @03:19:19.76 AS.seek 0ml @03:19:47.09 AFV.select :carrierSolution @03:19:47.19 delay 2 seconds @03:19:49.22 AS.reconfigure ASconfig @03:19:49.27 AS.seek 1ml @03:20:47.05 delay 1 second @03:20:48.07 AFV.select :holdingCoil @03:20:48.17 delay 1 second @03:20:49.20 AS.reconfigure ASfast @03:20:49.25 AS.seek 0ml @03:21:16.58 ARV.dial between carrier and air1 @03:21:17.08 ATV.dial :air3 @03:21:18.37 AS.reconfigure ASslow1 @03:21:18.41 AS.seek 0.01ml @03:21:20.78 delay 2 seconds @03:21:22.80 ATV.dial :bleach @03:21:23.40 AS.seek 0.81ml @03:24:04.58 delay 3 seconds @03:24:07.60 ATV.dial :air3 @03:24:08.14 delay 3 seconds @03:24:11.21 AS.seek 0.835ml @03:24:16.58 delay 3 seconds @03:24:19.60 ATV.dial :bleach @03:24:20.20 AS.seek 0.82ml @03:24:23.51 delay 3 seconds @03:24:26.53 ATV.dial PCR @03:24:26.94 AS.reconfigure ASconfig @03:24:26.99 AS.seek :empty @03:25:14.45 PCR.program :bleachProfile @03:25:14.49 PCR program should complete in < 2:45 @03:25:14.50 PCR.run 10:00 @03:28:13.79 PCR.off @03:28:13.82 AFV.select :carrierSolution @03:28:13.92 delay 2 seconds @03:28:15.95 AS.seek 1ml @03:29:13.76 delay 1 second @03:29:14.78 AFV.select :holdingCoil @03:29:14.88 delay 1 second @03:29:15.91 AS.reconfigure ASfast @03:29:15.96 AS.seek 0ml @03:29:43.30 delay 3 seconds @03:29:46.32 AFV.select :carrierSolution @03:29:46.42 delay 2 seconds @03:29:48.45 AS.reconfigure ASconfig @03:29:48.51 AS.seek 1ml @03:30:46.26 delay 1 second @03:30:47.28 AFV.select :holdingCoil @03:30:47.38 delay 1 second @03:30:48.41 AS.reconfigure ASfast @03:30:48.46 AS.seek 0ml @03:31:15.78 delay 3 seconds @03:31:18.80 AFV.select :carrierSolution @03:31:18.90 delay 2 seconds @03:31:20.93 AS.reconfigure ASconfig @03:31:20.99 AS.seek 1ml @03:32:18.73 delay 1 second @03:32:19.75 AFV.select :holdingCoil @03:32:19.85 delay 1 second @03:32:20.88 AS.reconfigure ASfast @03:32:20.93 AS.seek 0ml @03:32:48.26 delay 3 seconds @03:32:51.28 AFV.select :carrierSolution @03:32:51.38 delay 2 seconds @03:32:53.41 AS.reconfigure ASconfig @03:32:53.45 AS.seek 1ml @03:33:51.20 delay 1 second @03:33:52.22 AFV.select :holdingCoil @03:33:52.32 delay 1 second @03:33:53.35 AS.reconfigure ASfast @03:33:53.40 AS.seek 0ml @03:34:20.72 delay 3 seconds @03:34:23.74 AFV.select :carrierSolution @03:34:23.84 delay 2 seconds @03:34:25.87 AS.reconfigure ASconfig @03:34:25.93 AS.seek 1ml @03:35:23.67 delay 1 second @03:35:24.69 AFV.select :holdingCoil @03:35:24.79 delay 1 second @03:35:25.82 AS.reconfigure ASnorm @03:35:25.87 AS.seek 0ml @03:36:16.59 AFV.select :carrierSolution @03:36:16.69 delay 2 seconds @03:36:18.72 AS.reconfigure ASconfig @03:36:18.76 AS.seek 1ml @03:37:16.51 delay 1 second @03:37:17.53 AFV.select :holdingCoil @03:37:17.63 delay 1 second @03:37:18.65 ATV.dial :waste @03:37:19.90 AS.reconfigure ASnorm @03:37:19.94 AS.seek 0.5ml @03:37:45.52 ATV.dial :waste2 @03:37:46.82 AS.seek 0ml @03:38:12.34
Asleep after completing 190517totalsampletest assays @03:38:12.35 Mission runtime was 11:14:21.398441 @03:38:12.37 GoodNews.email "{1 => 3, 2 => 0}\nCan@03:33:55, 21.7C, 40% humidity, 14.6psia, 12.060V, 0.449A, 0.449A avg, 5.42W",Subject: "Asleep after completing 190517totalsampletest assays" @15:48:13.76CEST20-May-19
ESP2 v3.30 I2C Gateway v3.06 for core bus @15:48:15.43 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @15:48:15.68 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @15:48:19.89 Polling Can every 10:00 @15:48:21.66 SPE.coast @15:48:21.70 SPE.configure SPEconfig @15:48:21.79 AS.configure ASconfig @15:48:21.87 SS.reconfigure SSconfig @15:48:21.91 SS.hold @15:48:21.97 SS.configure SSconfig @15:48:22.05 CC.reconfigure CCconfig @15:48:22.09 CC.hold @15:48:22.16 CC.configure CCconfig @15:48:22.24 CS.reconfigure CSconfig @15:48:22.28 CS.hold @15:48:22.35 CS.configure CSconfig @15:48:22.43 PC.reconfigure PCconfig @15:48:22.47 PC.hold @15:48:22.87 PC.configure PCconfig @15:48:22.95 PS.configure PSconfig @15:48:23.05 Forearm.reconfigure LooseForearm @15:48:23.09 Forearm.hold @15:48:23.13 Forearm.configure LooseForearm @15:48:23.21 Elbow.reconfigure LooseElbow @15:48:23.25 Elbow.hold @15:48:23.30 Elbow.configure LooseElbow @15:48:23.40 SC.reconfigure SCloose @15:48:23.44 SC.hold @15:48:23.49 SC.configure SCloose @15:48:23.57 SE.reconfigure SEconfig @15:48:23.60 SE.hold @15:48:23.66 SE.configure SEconfig @15:48:23.70 CH.coast @15:48:23.75 CH.configure CHconfig @15:48:23.77 PH.coast @15:48:23.82 PH.configure PHconfig @15:48:23.86 PCR.off @15:48:23.88 PCR.pidtemp= 50,0.1,0,300,25,25 @15:48:23.91 PCR.caltemp= 2.8814,0.0372,0 @15:48:23.94 All dwarf controllers running firmware version 3.75 @15:48:24.05 Cleanly exited at 15:45:34CEST20-May-19 @15:48:24.08 Loading /home/esp/esp2/mission/TotalWCRTest.rb mission @15:48:24.12 RuntimeError in MAIN -- Mission's startTube should be 3, not 2 @15:48:24.14 Waiting to Resume from Checkpoint "Loading /home/esp/esp2/mission/TotalWCRTest.rb mission" @15:48:24.15 BadNews.email "Mission's startTube should be 3, not 2",Subject: "RuntimeError in MAIN" @15:48:24.72 Session Begun @15:50:14.27 -> abort! "forget ESP.ready in script I think" @15:50:14.28 CmdServer::Client::Error in mwj -- Aborting Mission: forget ESP.ready in script I think @15:50:14.34 BadNews.email "forget ESP.ready in script I think",Subject: "TotalWCRTest ABORTED!" @15:50:20.43 Session Ended @15:53:24.41CEST20-May-19
ESP2 v3.30 I2C Gateway v3.06 for core bus @15:53:26.06 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @15:53:26.31 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @15:53:30.95 Polling Can every 10:00 @15:53:32.71 SPE.coast @15:53:32.76 SPE.configure SPEconfig @15:53:32.84 AS.configure ASconfig @15:53:32.92 SS.reconfigure SSconfig @15:53:32.96 SS.hold @15:53:33.02 SS.configure SSconfig @15:53:33.11 CC.reconfigure CCconfig @15:53:33.14 CC.hold @15:53:33.19 CC.configure CCconfig @15:53:33.27 CS.reconfigure CSconfig @15:53:33.32 CS.hold @15:53:33.38 CS.configure CSconfig @15:53:33.46 PC.reconfigure PCconfig @15:53:33.50 PC.hold @15:53:33.90 PC.configure PCconfig @15:53:33.98 PS.configure PSconfig @15:53:34.08 Forearm.reconfigure LooseForearm @15:53:34.12 Forearm.hold @15:53:34.17 Forearm.configure LooseForearm @15:53:34.25 Elbow.reconfigure LooseElbow @15:53:34.29 Elbow.hold @15:53:34.34 Elbow.configure LooseElbow @15:53:34.44 SC.reconfigure SCloose @15:53:34.48 SC.hold @15:53:34.54 SC.configure SCloose @15:53:34.62 SE.reconfigure SEconfig @15:53:34.66 SE.hold @15:53:34.71 SE.configure SEconfig @15:53:34.75 CH.coast @15:53:34.80 CH.configure CHconfig @15:53:34.83 PH.coast @15:53:34.87 PH.configure PHconfig @15:53:34.91 PCR.off @15:53:34.93 PCR.pidtemp= 50,0.1,0,300,25,25 @15:53:34.96 PCR.caltemp= 2.8814,0.0372,0 @15:53:34.99 All dwarf controllers running firmware version 3.75 @15:53:35.12 Loading /home/esp/esp2/mission/TotalWCRTest.rb mission @15:53:35.19 Preparing to sleep until +00:00:10 @15:53:35.20 GoodNews.email "Starting TotalWCRTest w/pucks in tube 2\n{}\nCan@15:53:30, 23.0C, 47% humidity, 14.5psia, 11.957V, 0.758A, 0.758A avg, 9.07W",Subject: "TotalWCRTest begun" @15:53:35.27 SS.coast @15:53:35.30
Asleep til +00:00:10 @15:53:35.34 Continuing at 15:53 @15:53:35.35 CC.coast @15:53:35.43 CS.coast @15:53:35.46 PC.coast @15:53:35.50 Forearm.coast @15:53:35.52 Elbow.coast @15:53:35.55 SC.coast @15:53:35.58 SE.coast @15:53:35.97 Gate.power :core,:OFF @15:53:35.98 Gate.power :raw,:OFF @15:53:35.99 Shutdown @15:53:37.33 Session Begun @15:53:40.91 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotalWCRTest.rb:113\n{}\nCan@15:53:35, 23.0C, 47% humidity, 14.5psia, 11.957V, 0.764A, 0.758A avg, 9.14W",Subject: "Asleep until 15:53" @15:53:45.16
Powering core bus @15:53:49.86 Gate.power :raw,:ON @15:53:50.48 SPE.coast @15:53:50.54 SPE.configure SPEconfig @15:53:50.64 AS.configure ASconfig @15:53:50.74 SS.configure SSconfig @15:53:50.85 CC.configure CCconfig @15:53:50.94 CS.configure CSconfig @15:53:51.05 PC.configure PCconfig @15:53:51.15 PS.configure PSconfig @15:53:51.27 Forearm.configure LooseForearm @15:53:51.37 Elbow.configure LooseElbow @15:53:51.49 SC.configure SCloose @15:53:51.59 SE.configure SEconfig @15:53:51.65 CH.coast @15:53:51.71 CH.configure CHconfig @15:53:51.75 PH.coast @15:53:51.82 PH.configure PHconfig @15:53:51.87 PCR.off @15:53:51.91 PCR.pidtemp= 50,0.1,0,300,25,25 @15:53:51.95 PCR.caltemp= 2.8814,0.0372,0 @15:53:51.99 All dwarf controllers running firmware version 3.75 @15:53:52.10 Valve.reset @15:53:52.27 PCR.off @15:53:52.31 PCR.pidtemp= 50,0.1,0,300,25,25 @15:53:52.34 PCR.caltemp= 2.8814,0.0372,0 @15:53:52.48 Intake.select :close @15:53:52.52 Exhaust.select :close @15:53:52.54 CTV.dial :air @15:53:52.66 CSV.dial :waste1 @15:53:52.70 PSV.dial :air @15:53:52.83
Forearm.reconfigure LooseForearm @15:53:52.91 Forearm.home.seek :retract @15:53:52.96 PS.reconfigure PSconfig @15:53:53.03 PS.home.seek 0ml @15:53:53.09 SSV.dial :spit,avoiding: :exhaust @15:53:53.57 CS.reconfigure CSfast @15:53:53.60 SS.reconfigure SSconfig @15:53:53.73 CS.home.seek 0ml @15:53:53.77 SS.home.seek 0ml @15:53:53.89 Hand.close @15:53:53.94
Elbow.jog 1500 @15:53:54.45 Elbow.reconfigure LooseElbow @15:53:54.51 Elbow.home.seek :clear @15:53:59.21 SE.reconfigure SEconfig @15:53:59.28 SE.home.seek :down @15:54:00.04 SC.reconfigure SCsloppy @15:54:00.09 SC.home.seek :initial @15:54:02.27 PS.coast @15:54:10.65
Forearm.seek :retract @15:54:10.74 Elbow.seek :hammer @15:54:11.96 Forearm.reconfigure TightForearm @15:54:12.03 Forearm.seek 5 + 350 counts @15:54:13.10 Forearm.seek :retract @15:54:14.29 PC.reconfigure PCconfig @15:54:14.31
CC.reconfigure CCconfig @15:54:14.40 PC.home.seek :open @15:54:14.43
CC.home.seek :open @15:54:17.69 Phase [wcrPhase2] @15:54:17.71 GoodNews.email "At 15:54:17 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotalWCRTest.rb:113\n{}\nCan@15:54:17, 23.1C, 47% humidity, 14.5psia, 11.982V, 0.651A, 0.651A avg, 7.80W",Subject: "Started TotalWCRTest phase wcrPhase2" @15:54:18.28CEST20-May-19
==================> STARTED WCRSphase (phase #1) <=== @15:54:18.29 Starting stand-alone bac WCR @15:54:18.31 bac wcr sampling at most 2000ml @15:54:18.37 Whole Cell Archive sampling 2000ml @15:54:18.38 Loading wcr puck from tube 2 into Collection Clamp @15:54:18.47 Hand.open @15:54:19.38 SC.reconfigure SCloose @15:54:19.56 Elbow.reconfigure TightElbow @15:54:19.63 Elbow.seek 2 @15:54:20.03 Forearm.seek 2 + 700 counts @15:54:21.21 SE.reconfigure SEpick @15:54:21.30 SE.seek beamBreak @15:54:23.50 BACfans.email "Started stand-alone bac WCR in tube 2 at 15:54:18CEST20-May-19\n{}\nCan@15:54:17, 23.1C, 47% humidity, 14.5psia, 11.982V, 0.651A, 0.651A avg, 7.80W",Subject: "bac wcr sampling at most 2000ml" @15:54:24.39 SE.configure SEconfig @15:54:24.58 Elbow.seek 2 @15:54:24.80 Forearm.seek 2 + 300 counts @15:54:25.00 Forearm.reconfigure LooseForearm @15:54:25.06 Hand.close @15:54:25.82 SE.seek :down @15:54:26.06 Forearm.seek :retract @15:54:27.05 Elbow.seek CC @15:54:28.72 Elbow.reconfigure LooseElbow @15:54:28.80 SE.coast @15:54:28.92 Forearm.reconfigure TightForearm @15:54:29.00 Forearm.seek CC @15:54:30.72 CC.seek :closed @15:54:35.05 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.34 @15:54:35.07 Hand.open @15:54:35.85 Forearm.reconfigure LooseForearm @15:54:35.92 Forearm.seek :retract @15:54:37.00 SC.coast @15:54:37.05 collectSample 2000ml w/bubble point of 55psi @15:54:37.07 Priming 7ml @15:54:37.08 Intake.select :close @15:54:37.11 Exhaust.select :close @15:54:37.14 SS.hold @15:54:37.36 Calibrating pressure and temperature sensors @15:54:37.38 CTV.dial :air @15:54:37.41 SSV.dial :spit,avoiding: :exhaust @15:54:37.45 SS.reconfigure SSfast @15:54:37.52 SS.seek 5ml @15:54:40.39 SSV.dial CBV @15:54:40.42 CBV.dial SSV @15:54:41.95 delay 3.8 seconds @15:54:45.87 SSV.dial :spit @15:54:47.39 SS.seek 0ml @15:54:50.38 SSV.dial CBV @15:54:51.86 CTV.dial :intake @15:54:52.76 SSV.dial CBV @15:54:52.79 CBV.dial SSV @15:54:52.81 Allowing 40s for 7.0ml of remaining 7.0ml @15:54:52.82 Intake.select :open @15:54:55.01 Intake @15.7psia, vacuum from 9.2 to 13.2psi @15:54:55.03 SS.reconfigure SSinhale.with maxSpeed: 150 @15:54:55.10 SS.seek 7ml @15:55:00.69 Stroke completed -- waiting for pressure to equalize ... @15:55:20.73 Intake.select :close @15:55:21.24 SSV.dial :exhaust @15:55:22.18 Exhaust.select :open @15:55:22.31 SS.reconfigure SSexhale @15:55:22.38 SS.seek 0ml @15:55:26.36 Exhaust.select :close @15:55:26.49 Sampling 2000.0ml @15:55:26.50 Intake.select :close @15:55:26.53 Exhaust.select :close @15:55:26.55 SS.reconfigure SSconfig @15:55:26.63 SS.hold @15:55:26.84 CTV.dial :intake @15:55:26.85 SSV.dial CBV @15:55:26.89 CBV.dial SSV @15:55:27.77 Allowing 130s for 25.0ml of remaining 2000.0ml @15:55:27.79 Intake.select :open @15:55:29.97 Intake @15.6psia, vacuum from 9.1 to 13.1psi @15:55:29.99 SS.reconfigure SSinhale.with maxSpeed: 150 @15:55:30.07 SS.seek 25ml @15:55:34.65 Vacuum too high -- waiting up to 40s @15:55:42.79 SS.reconfigure SSinhale.with maxSpeed: 135 @15:55:42.86 SS.seek 25ml @15:55:46.05 Vacuum too high -- waiting up to 40s @15:55:54.38 SS.reconfigure SSinhale.with maxSpeed: 122 @15:55:54.45 SS.seek 25ml @15:55:57.52 Vacuum too high -- waiting up to 40s @15:56:05.19 SS.reconfigure SSinhale.with maxSpeed: 110 @15:56:05.26 SS.seek 25ml @15:56:08.30 Vacuum too high -- waiting up to 40s @15:56:16.63 Intake @15.6psia, vacuum from 9.1 to 13.1psi @15:56:16.65 SS.reconfigure SSinhale.with maxSpeed: 99 @15:56:16.73 SS.seek 25ml @15:56:20.68 Vacuum too high -- waiting up to 40s @15:56:29.01 Intake @15.6psia, vacuum from 9.1 to 13.1psi @15:56:29.03 SS.reconfigure SSinhale.with maxSpeed: 89 @15:56:29.11 SS.seek 25ml @15:56:32.68 Vacuum too high -- waiting up to 40s @15:56:41.02 Intake @15.7psia, vacuum from 9.2 to 13.2psi @15:56:41.04 SS.reconfigure SSinhale.with maxSpeed: 80 @15:56:41.12 SS.seek 25ml @15:56:43.56 Stroke completed -- waiting for pressure to equalize ... @15:56:51.35 Intake.select :close @15:56:51.52 SSV.dial :exhaust @15:56:52.39 Exhaust.select :open @15:56:52.52 SS.reconfigure SSexhale @15:56:52.59 SS.seek 0ml @15:57:01.77 Exhaust.select :close @15:57:01.90 SSV.dial CBV @15:57:01.92 CBV.dial SSV @15:57:02.80 Allowing 130s for 25.0ml of remaining 1975.0ml @15:57:02.82 Intake.select :open @15:57:05.01 Intake @15.6psia, vacuum from 9.1 to 13.1psi @15:57:05.03 SS.reconfigure SSinhale.with maxSpeed: 80 @15:57:05.11 SS.seek 25ml @15:57:09.99 Vacuum too high -- waiting up to 40s @15:57:15.18 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:57:15.20 SS.reconfigure SSinhale.with maxSpeed: 72 @15:57:15.27 SS.seek 25ml @15:57:18.65 Vacuum too high -- waiting up to 40s @15:57:24.67 Intake @15.6psia, vacuum from 9.1 to 13.1psi @15:57:24.69 SS.reconfigure SSinhale.with maxSpeed: 65 @15:57:24.76 SS.seek 25ml @15:57:29.27 Vacuum too high -- waiting up to 40s @15:57:35.32 Intake @15.6psia, vacuum from 9.1 to 13.1psi @15:57:35.34 SS.reconfigure SSinhale.with maxSpeed: 59 @15:57:35.42 SS.seek 25ml @15:57:41.08 Vacuum too high -- waiting up to 40s @15:57:48.03 Intake @15.6psia, vacuum from 9.1 to 13.1psi @15:57:48.05 SS.reconfigure SSinhale.with maxSpeed: 53 @15:57:48.12 SS.seek 25ml @15:57:54.58 Vacuum too high -- waiting up to 40s @15:58:01.10 Intake @15.6psia, vacuum from 9.1 to 13.1psi @15:58:01.12 SS.reconfigure SSinhale.with maxSpeed: 48 @15:58:01.20 SS.seek 25ml @15:58:08.65 Vacuum too high -- waiting up to 40s @15:58:15.36 Intake @15.6psia, vacuum from 9.1 to 13.1psi @15:58:15.39 SS.reconfigure SSinhale.with maxSpeed: 43 @15:58:15.46 SS.seek 25ml @15:58:18.31 Stroke completed -- waiting for pressure to equalize ... @15:58:25.07 Intake.select :close @15:58:25.22 SSV.dial :exhaust @15:58:26.09 Exhaust.select :open @15:58:26.21 SS.reconfigure SSexhale @15:58:26.27 SS.seek 0ml @15:58:35.81 Exhaust.select :close @15:58:35.94 SSV.dial CBV @15:58:35.96 CBV.dial SSV @15:58:36.84 Allowing 130s for 25.0ml of remaining 1950.0ml @15:58:36.86 Intake.select :open @15:58:39.05 Intake @15.6psia, vacuum from 9.1 to 13.1psi @15:58:39.07 SS.reconfigure SSinhale.with maxSpeed: 43 @15:58:39.15 SS.seek 25ml @15:58:46.55 Vacuum too high -- waiting up to 40s @15:58:51.69 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:58:51.71 SS.reconfigure SSinhale.with maxSpeed: 39 @15:58:51.78 SS.seek 25ml @15:58:58.77 Vacuum too high -- waiting up to 40s @15:59:04.58 SS.reconfigure SSinhale.with maxSpeed: 35 @15:59:04.65 SS.seek 25ml @15:59:13.96 Vacuum too high -- waiting up to 40s @15:59:20.94 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:59:20.96 SS.reconfigure SSinhale.with maxSpeed: 32 @15:59:21.04 SS.seek 25ml @15:59:32.49 Vacuum too high -- waiting up to 40s @15:59:40.36 Intake @15.7psia, vacuum from 9.2 to 13.2psi @15:59:40.38 SS.reconfigure SSinhale.with maxSpeed: 29 @15:59:40.46 SS.seek 25ml @15:59:57.49 Vacuum too high -- waiting up to 40s @16:00:06.07 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:00:06.09 SS.reconfigure SSinhale.with maxSpeed: 26 @16:00:06.18 SS.seek 25ml @16:00:09.37 Stroke completed -- waiting for pressure to equalize ... @16:00:15.95 Intake.select :close @16:00:16.12 SSV.dial :exhaust @16:00:16.98 Exhaust.select :open @16:00:17.10 SS.reconfigure SSexhale @16:00:17.16 SS.seek 0ml @16:00:27.08 Exhaust.select :close @16:00:27.21 SSV.dial CBV @16:00:27.23 CBV.dial SSV @16:00:28.12 Allowing 130s for 25.0ml of remaining 1925.0ml @16:00:28.14 Intake.select :open @16:00:30.33 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:00:30.35 SS.reconfigure SSinhale.with maxSpeed: 26 @16:00:30.43 SS.seek 25ml @16:00:48.83 Vacuum too high -- waiting up to 40s @16:00:54.19 Intake @15.4psia, vacuum from 8.9 to 12.9psi @16:00:54.21 SS.reconfigure SSinhale.with maxSpeed: 23 @16:00:54.29 SS.seek 25ml @16:01:31.39 Vacuum too high -- waiting up to 40s @16:01:39.25 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:01:39.27 SS.reconfigure SSinhale.with maxSpeed: 21 @16:01:39.35 SS.seek 25ml @16:02:01.47 Stroke completed -- waiting for pressure to equalize ... @16:02:11.24 Intake.select :close @16:02:11.39 SSV.dial :exhaust @16:02:12.27 Exhaust.select :open @16:02:12.40 SS.reconfigure SSexhale @16:02:12.47 SS.seek 0ml @16:02:22.09 Exhaust.select :close @16:02:22.22 SSV.dial CBV @16:02:22.24 CBV.dial SSV @16:02:23.11 Allowing 130s for 25.0ml of remaining 1900.0ml @16:02:23.13 Intake.select :open @16:02:25.31 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:02:25.33 SS.reconfigure SSinhale.with maxSpeed: 21 @16:02:25.40 SS.seek 25ml @16:03:49.78 Stroke completed -- waiting for pressure to equalize ... @16:04:04.25 Intake.select :close @16:04:04.42 SSV.dial :exhaust @16:04:05.28 Exhaust.select :open @16:04:05.41 SS.reconfigure SSexhale @16:04:05.48 SS.seek 0ml @16:04:14.73 Exhaust.select :close @16:04:14.86 SSV.dial CBV @16:04:14.88 CBV.dial SSV @16:04:15.76 Allowing 130s for 25.0ml of remaining 1875.0ml @16:04:15.78 Intake.select :open @16:04:17.97 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:04:17.99 SS.reconfigure SSinhale.with maxSpeed: 21 @16:04:18.07 SS.seek 25ml @16:05:10.64 Vacuum too high -- waiting up to 40s @16:05:18.53 Intake @15.3psia, vacuum from 8.8 to 12.8psi @16:05:18.55 SS.reconfigure SSinhale.with maxSpeed: 19 @16:05:18.63 SS.seek 25ml @16:05:55.37 Stroke completed -- waiting for pressure to equalize ... @16:06:06.78 Intake.select :close @16:06:06.95 SSV.dial :exhaust @16:06:07.83 Exhaust.select :open @16:06:07.95 SS.reconfigure SSexhale @16:06:08.01 SS.seek 0ml @16:06:17.26 Exhaust.select :close @16:06:17.39 SSV.dial CBV @16:06:17.41 CBV.dial SSV @16:06:18.29 Allowing 130s for 25.0ml of remaining 1850.0ml @16:06:18.31 Intake.select :open @16:06:20.49 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:06:20.51 SS.reconfigure SSinhale.with maxSpeed: 19 @16:06:20.59 SS.seek 25ml @16:07:54.28 Stroke completed -- waiting for pressure to equalize ... @16:08:09.54 Intake.select :close @16:08:09.69 SSV.dial :exhaust @16:08:10.57 Exhaust.select :open @16:08:10.70 SS.reconfigure SSexhale @16:08:10.76 SS.seek 0ml @16:08:20.04 Exhaust.select :close @16:08:20.17 SSV.dial CBV @16:08:20.19 CBV.dial SSV @16:08:21.06 Allowing 130s for 25.0ml of remaining 1825.0ml @16:08:21.08 Intake.select :open @16:08:23.26 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:08:23.28 SS.reconfigure SSinhale.with maxSpeed: 19 @16:08:23.36 SS.seek 25ml @16:09:57.03 Stroke completed -- waiting for pressure to equalize ... @16:10:12.70 Intake.select :close @16:10:12.87 SSV.dial :exhaust @16:10:13.73 Exhaust.select :open @16:10:13.86 SS.reconfigure SSexhale @16:10:13.93 SS.seek 0ml @16:10:23.59 Exhaust.select :close @16:10:23.72 SSV.dial CBV @16:10:23.74 CBV.dial SSV @16:10:24.62 Allowing 130s for 25.0ml of remaining 1800.0ml @16:10:24.64 Intake.select :open @16:10:26.83 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:10:26.85 SS.reconfigure SSinhale.with maxSpeed: 19 @16:10:26.93 SS.seek 25ml @16:11:35.67 Vacuum too high -- waiting up to 40s @16:11:43.80 Intake @15.3psia, vacuum from 8.8 to 12.8psi @16:11:43.82 SS.reconfigure SSinhale.with maxSpeed: 17 @16:11:43.90 SS.seek 25ml @16:12:13.18 Stroke completed -- waiting for pressure to equalize ... @16:12:23.57 Intake.select :close @16:12:23.73 SSV.dial :exhaust @16:12:24.60 Exhaust.select :open @16:12:24.72 SS.reconfigure SSexhale @16:12:24.78 SS.seek 0ml @16:12:34.74 Exhaust.select :close @16:12:34.87 SSV.dial CBV @16:12:34.89 CBV.dial SSV @16:12:35.76 Allowing 130s for 25.0ml of remaining 1775.0ml @16:12:35.78 Intake.select :open @16:12:37.97 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:12:37.99 SS.reconfigure SSinhale.with maxSpeed: 17 @16:12:38.07 SS.seek 25ml @16:14:23.17 Stroke completed -- waiting for pressure to equalize ... @16:14:36.55 Intake.select :close @16:14:36.70 SSV.dial :exhaust @16:14:37.56 Exhaust.select :open @16:14:37.68 SS.reconfigure SSexhale @16:14:37.74 SS.seek 0ml @16:14:47.07 Exhaust.select :close @16:14:47.20 SSV.dial CBV @16:14:47.22 CBV.dial SSV @16:14:48.10 Allowing 130s for 25.0ml of remaining 1750.0ml @16:14:48.12 Intake.select :open @16:14:50.31 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:14:50.33 SS.reconfigure SSinhale.with maxSpeed: 17 @16:14:50.41 SS.seek 25ml @16:16:35.76 Stroke completed -- waiting for pressure to equalize ... @16:16:49.65 Intake.select :close @16:16:49.81 SSV.dial :exhaust @16:16:50.68 Exhaust.select :open @16:16:50.80 SS.reconfigure SSexhale @16:16:50.87 SS.seek 0ml @16:17:00.16 Exhaust.select :close @16:17:00.29 SSV.dial CBV @16:17:00.31 CBV.dial SSV @16:17:01.20 Allowing 130s for 25.0ml of remaining 1725.0ml @16:17:01.22 Intake.select :open @16:17:03.41 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:17:03.43 SS.reconfigure SSinhale.with maxSpeed: 17 @16:17:03.51 SS.seek 25ml @16:18:49.48 Stroke completed -- waiting for pressure to equalize ... @16:19:04.72 Intake.select :close @16:19:04.89 SSV.dial :exhaust @16:19:05.76 Exhaust.select :open @16:19:05.88 SS.reconfigure SSexhale @16:19:05.95 SS.seek 0ml @16:19:15.60 Exhaust.select :close @16:19:15.73 SSV.dial CBV @16:19:15.75 CBV.dial SSV @16:19:16.62 Allowing 130s for 25.0ml of remaining 1700.0ml @16:19:16.64 Intake.select :open @16:19:18.83 Intake @15.3psia, vacuum from 8.8 to 12.8psi @16:19:18.85 SS.reconfigure SSinhale.with maxSpeed: 17 @16:19:18.93 SS.seek 25ml @16:21:04.79 Stroke completed -- waiting for pressure to equalize ... @16:21:18.67 Intake.select :close @16:21:18.82 SSV.dial :exhaust @16:21:19.68 Exhaust.select :open @16:21:19.80 SS.reconfigure SSexhale @16:21:19.87 SS.seek 0ml @16:21:29.19 Exhaust.select :close @16:21:29.32 SSV.dial CBV @16:21:29.34 CBV.dial SSV @16:21:30.23 Allowing 130s for 25.0ml of remaining 1675.0ml @16:21:30.25 Intake.select :open @16:21:32.44 Intake @15.4psia, vacuum from 8.9 to 12.9psi @16:21:32.46 SS.reconfigure SSinhale.with maxSpeed: 17 @16:21:32.54 SS.seek 25ml @16:22:35.64 Vacuum too high -- waiting up to 40s @16:22:43.06 Intake @15.2psia, vacuum from 8.7 to 12.7psi @16:22:43.08 SS.reconfigure SSinhale.with maxSpeed: 15 @16:22:43.15 SS.seek 25ml @16:23:31.94 Stroke completed -- waiting for pressure to equalize ... @16:23:40.26 SS.reconfigure SSconfig @16:23:40.32 SS.hold @16:23:40.45 Intake.select :close @16:23:40.60 ShallowSampler::Clogged in WCR -- progress too slow after 350.0ml @16:23:40.62 SSV.dial :exhaust @16:23:41.51 Exhaust.select :open @16:23:41.63 SS.reconfigure SSexhale @16:23:41.69 SS.seek 0ml @16:23:51.38 Exhaust.select :close @16:23:51.51 Sampled 350.0ml @16:23:51.53 Drying puck before fixing for 4:00 @16:23:51.55 CBV.dial :waste2b @16:23:52.43 delay 1:00 @16:24:52.47 CSV.dial :air @16:24:53.30 CS.seek 10ml @16:25:20.01 Pumping wcr puck from tube 2 up to 55psi... @16:25:20.03 CBV.dial SSV @16:25:21.48 CBV.dial between CSV and waste2b @16:25:22.57 CSV.dial between air and CTV @16:25:22.93 CS.seek 7.266ml @16:25:30.96 CTV.dial CSV,avoiding: :air @16:25:30.99 CSV.dial CTV @16:25:31.90 CS.reconfigure CSpressurize @16:25:31.97 CS.seek 0ml @16:25:51.26 CSV.dial :air @16:25:51.77 CS.reconfigure CSfast @16:25:51.83 CS.seek 10ml @16:26:18.36 CSV.dial between air and CTV @16:26:18.74 CS.seek 2.158ml @16:26:39.81 CTV.dial CSV,avoiding: :air @16:26:39.84 CSV.dial CTV @16:26:40.20 CS.reconfigure CSpressurize @16:26:40.27 CS.seek 0ml @16:26:42.19 CBV.dial :waste2b @16:26:42.57 CS.reconfigure CSconfig @16:26:42.63 CS.seek :empty @16:26:47.83 delay 1:00 @16:27:47.87 CTV.dial :air,avoiding: :intake @16:27:49.42 Fix #1 of 2 (with rnal) for 2:00 @16:27:49.44 CSV.dial CSR @16:27:50.01 Pulling 2ml of CSR.rnal @16:27:50.02 CSR.select :rnal @16:27:50.17 CS.seek 2ml @16:27:56.42 delay 5 seconds @16:28:01.44 CSR.select :air @16:28:01.58 CS.reconfigure CSfast @16:28:01.65 CS.seek 10ml @16:28:23.11 Pumping wcr puck from tube 2 up to 55psi... @16:28:23.13 CBV.dial SSV @16:28:24.97 CBV.dial between CSV and waste2b @16:28:26.11 CSV.dial between air and CTV @16:28:26.81 CS.seek 7.279ml @16:28:34.81 CTV.dial CSV,avoiding: :air @16:28:34.84 CSV.dial CTV @16:28:36.42 CS.reconfigure CSpressurize @16:28:36.49 CS.seek 0ml @16:28:55.80 CSV.dial :air @16:28:56.35 CS.reconfigure CSfast @16:28:56.42 CS.seek 10ml @16:29:23.01 CSV.dial between air and CTV @16:29:23.38 CS.seek 2.067ml @16:29:44.74 CTV.dial CSV,avoiding: :air @16:29:44.77 CSV.dial CTV @16:29:45.14 CS.reconfigure CSpressurize @16:29:45.21 CS.seek 0ml @16:29:46.54 delay 2:00 @16:31:46.57 CBV.dial :waste2b @16:31:46.93 CS.reconfigure CSconfig @16:31:46.99 CS.seek :empty @16:31:52.58 delay 1:00 @16:32:52.62 CTV.dial :air,avoiding: :intake @16:32:54.15 Fix #2 of 2 (with rnal) for 2:00 @16:32:54.16 CSV.dial CSR @16:32:54.73 Pulling 2ml of CSR.rnal @16:32:54.74 CSR.select :rnal @16:32:54.89 CS.seek 2ml @16:33:00.99 delay 5 seconds @16:33:06.01 CSR.select :air @16:33:06.15 CS.reconfigure CSfast @16:33:06.21 CS.seek 10ml @16:33:27.46 Pumping wcr puck from tube 2 up to 55psi... @16:33:27.48 CBV.dial SSV @16:33:28.91 CBV.dial between CSV and waste2b @16:33:30.02 CSV.dial between air and CTV @16:33:30.73 CS.seek 7.293ml @16:33:38.62 CTV.dial CSV,avoiding: :air @16:33:38.65 CSV.dial CTV @16:33:40.23 CS.reconfigure CSpressurize @16:33:40.30 CS.seek 0ml @16:33:59.61 CSV.dial :air @16:34:00.26 CS.reconfigure CSfast @16:34:00.33 CS.seek 10ml @16:34:26.74 CSV.dial between air and CTV @16:34:27.11 CS.seek 2.076ml @16:34:48.59 CTV.dial CSV,avoiding: :air @16:34:48.62 CSV.dial CTV @16:34:48.98 CS.reconfigure CSpressurize @16:34:49.05 CS.seek 0ml @16:34:50.42 delay 2:00 @16:36:50.44 CBV.dial :waste2b @16:36:51.23 CS.reconfigure CSconfig @16:36:51.30 CS.seek :empty @16:36:56.96 delay 1:00 @16:37:57.00 CTV.dial :air,avoiding: :intake @16:37:58.59 Storing wcr puck in Collection Clamp to tube 1 @16:37:58.77 SC.reconfigure SCtight @16:37:58.83 SC.seek 1 @16:38:00.97 SC.reconfigure SCloose @16:38:01.19 Elbow.reconfigure TightElbow @16:38:01.26 Elbow.seek 1 @16:38:03.26 Forearm.reconfigure TightForearm @16:38:03.33 Forearm.seek 1 + 700 counts @16:38:04.37 SE.reconfigure SEpick @16:38:04.46 SE.seek beamBreak @16:38:07.43 SE.configure SEconfig @16:38:07.50 SE.seek :place @16:38:08.14 Forearm.reconfigure LooseForearm @16:38:08.22 Forearm.seek :retract @16:38:09.16 Elbow.seek CC @16:38:10.84 Elbow.reconfigure LooseElbow @16:38:10.95 Forearm.seek CC @16:38:12.00 Hand.close @16:38:12.73 CC.seek :open @16:38:17.24 Forearm.seek :retract @16:38:18.32 Elbow.reconfigure TightElbow @16:38:18.38 Elbow.seek 1 @16:38:20.40 Elbow.reconfigure LimpElbow @16:38:20.47 Forearm.seek 1 @16:38:21.51 Elbow.hold @16:38:21.60 Elbow.reconfigure TightElbow @16:38:21.66 Hand.open @16:38:22.59 Forearm.force -20 @16:38:22.65 Forearm.seek 1 + 347 counts @16:38:22.98 Forearm.reconfigure TightForearm @16:38:23.05 Forearm.seek 1 + 700 counts @16:38:23.33 SE.seek :down @16:38:24.46 Forearm.reconfigure LooseForearm @16:38:24.53 Forearm.seek :retract @16:38:26.21 SE.coast @16:38:26.26 SC.coast @16:38:26.31 Starting finalPurge @16:38:26.33 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:38:26.41 Hand.open @16:38:27.26 Elbow.seek FlushPuck::Garage @16:38:29.39 Elbow.reconfigure LooseElbow @16:38:29.50 Forearm.seek :garage @16:38:30.53 Hand.close @16:38:31.46 Forearm.seek :retract @16:38:32.59 Elbow.reconfigure TightElbow @16:38:32.65 Elbow.seek CC @16:38:35.50 Elbow.reconfigure LooseElbow @16:38:35.61 Forearm.reconfigure TightForearm @16:38:35.67 Forearm.seek CC @16:38:36.99 CC.seek :closed @16:38:41.33 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.42 @16:38:41.42 Hand.open @16:38:42.28 Forearm.reconfigure LooseForearm @16:38:42.34 Forearm.seek :retract @16:38:43.39 Flushing the Collection Valve::Manifold @16:38:43.53 CSV.dial CSR @16:38:44.08 Pulling 5ml of CSR.flush @16:38:44.09 CSR.select :flush @16:38:44.24 CS.seek 5ml @16:38:58.09 delay 5 seconds @16:39:03.10 CSR.select :air @16:39:03.28 CS.reconfigure CSfast @16:39:03.34 CS.seek 7.5ml @16:39:10.71 CSV.dial :waste @16:39:12.22 CS.reconfigure CSconfig @16:39:12.28 CS.seek 0ml @16:39:32.43 Flushing lower manifold's waste1 port @16:39:32.57 CSV.dial :flush @16:39:33.08 CS.reconfigure CSfast @16:39:33.14 CS.seek 5ml @16:39:46.83 delay 5 seconds @16:39:51.86 CSV.dial :air @16:39:52.42 CS.seek 7ml @16:39:58.49 CSV.dial CTV @16:39:58.52
CTV.dial CSV @16:40:00.12 CBV.dial :waste1 @16:40:01.00 CS.seek 0ml @16:40:19.80 Flushing lower manifold's waste2b port @16:40:19.94 CSV.dial :flush @16:40:20.80 CS.seek 5ml @16:40:34.43 delay 5 seconds @16:40:39.46 CSV.dial :air @16:40:40.06 CS.seek 7ml @16:40:46.12 CSV.dial CTV @16:40:46.15
CTV.dial CSV @16:40:46.65 CBV.dial :waste2b @16:40:47.53 CS.seek 0ml @16:41:06.24 Flushing upper manifold @16:41:06.38 CSV.dial :flush @16:41:07.25 CS.seek 5ml @16:41:20.87 delay 5 seconds @16:41:25.89 CSV.dial :air @16:41:26.95 CS.seek 7ml @16:41:33.15 CSV.dial CBV @16:41:33.19
CBV.dial CSV @16:41:34.43 CTV.dial :air @16:41:35.95 CS.seek 0ml @16:41:54.65 delay 7 seconds @16:42:01.68 CS.seek 3ml @16:42:10.24 CSV.dial :waste @16:42:10.77 CS.seek 0ml @16:42:19.41 CSV.dial :flush @16:42:19.92 CS.seek 3ml @16:42:28.52 delay 5 seconds @16:42:33.55 CSV.dial :air @16:42:34.11 CS.seek 5.001ml @16:42:40.12 CSV.dial :air @16:42:40.15 CS.seek 0ml @16:42:53.93 delay 7 seconds @16:43:00.97 CS.seek 3ml @16:43:09.52 CSV.dial :waste @16:43:10.38 CS.seek 0ml @16:43:18.90 Disinfecting external Intake with mfbkill @16:43:19.04 CSV.dial CSR @16:43:20.72 Pulling 8ml of CSR.mfbkill @16:43:20.73 CSR.select :mfbkill @16:43:20.88 CS.seek 8ml @16:43:42.16 delay 5 seconds @16:43:47.17 CSR.select :air @16:43:47.30 CS.seek 10ml @16:43:53.49 CSV.dial CBV @16:43:53.52
CBV.dial CSV @16:43:54.68 CTV.dial :intake @16:43:55.54 Intake.select :open @16:43:55.68 CS.seek 2ml @16:44:17.99 Intake.select :close @16:44:18.11 CTV.dial :air @16:44:18.99 CS.reconfigure CSconfig @16:44:19.05 CS.seek 0ml @16:44:25.21 Flushing lower manifold's waste1 port @16:44:25.22 CSV.dial CSR @16:44:26.49 Pulling 4ml of CSR.mfbkill @16:44:26.50 CSR.select :mfbkill @16:44:26.65 CS.seek 4ml @16:44:38.07 delay 5 seconds @16:44:43.08 CSR.select :air @16:44:43.22 CS.reconfigure CSfast @16:44:43.29 CS.seek 10ml @16:44:59.56 CSV.dial CTV @16:44:59.59
CTV.dial CSV @16:45:01.20 CBV.dial :waste1 @16:45:01.75 CS.seek 0ml @16:45:28.02 Flushing lower manifold's waste2b port @16:45:28.03 CSV.dial CSR @16:45:28.62 Pulling 4ml of CSR.mfbkill @16:45:28.63 CSR.select :mfbkill @16:45:28.77 CS.reconfigure CSconfig @16:45:28.83 CS.seek 4ml @16:45:40.06 delay 5 seconds @16:45:45.08 CSR.select :air @16:45:45.22 CS.reconfigure CSfast @16:45:45.29 CS.seek 10ml @16:46:01.49 CSV.dial CTV @16:46:01.52
CTV.dial CSV @16:46:02.04 CBV.dial :waste2b @16:46:02.94 CS.seek 0ml @16:46:29.43 Killing upper manifold @16:46:29.44 CSV.dial CSR @16:46:30.04 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @16:46:30.18 CS.reconfigure CSconfig @16:46:30.25 CS.seek 4ml @16:46:41.43 delay 5 seconds @16:46:46.45 CSR.select :air @16:46:46.59 CS.reconfigure CSfast @16:46:46.66 CS.seek 10ml @16:47:02.93 CSV.dial CBV @16:47:02.96
CBV.dial CSV @16:47:04.17 CTV.dial :air @16:47:06.16 CS.seek 0ml @16:47:32.34 delay 7 seconds @16:47:39.37 CS.seek 3ml @16:47:47.96 CSV.dial :waste @16:47:48.47 CS.seek 0ml @16:47:57.06 Flushing upper manifold @16:47:57.20 CSV.dial :flush @16:47:57.72 CS.seek 5ml @16:48:11.31 delay 5 seconds @16:48:16.34 CSV.dial :air @16:48:16.91 CS.seek 7ml @16:48:23.03 CSV.dial CBV @16:48:23.05
CBV.dial CSV @16:48:24.25 CTV.dial :air @16:48:24.28 CS.seek 0ml @16:48:43.00 delay 7 seconds @16:48:50.04 CS.seek 3ml @16:48:58.56 CSV.dial :waste @16:48:59.08 CS.seek 0ml @16:49:07.76 CSV.dial :flush @16:49:08.27 CS.seek 3ml @16:49:16.84 delay 5 seconds @16:49:21.86 CSV.dial :air @16:49:22.46 CS.seek 5ml @16:49:28.46 CSV.dial :air @16:49:28.49 CS.seek 0ml @16:49:42.15 delay 7 seconds @16:49:49.18 CS.seek 3ml @16:49:57.75 CSV.dial :waste @16:49:58.62 CS.seek 0ml @16:50:07.18 Flushing lower manifold's waste1 port @16:50:07.32 CSV.dial :flush @16:50:07.83 CS.seek 5ml @16:50:21.47 delay 5 seconds @16:50:26.49 CSV.dial :air @16:50:27.07 CS.seek 7ml @16:50:33.18 CSV.dial CTV @16:50:33.21
CTV.dial CSV @16:50:34.82 CBV.dial :waste1 @16:50:35.35 CS.seek 0ml @16:50:54.09 Flushing lower manifold's waste2b port @16:50:54.23 CSV.dial :flush @16:50:55.08 CS.seek 5ml @16:51:08.75 delay 5 seconds @16:51:13.77 CSV.dial :air @16:51:14.36 CS.seek 7ml @16:51:20.40 CSV.dial CTV @16:51:20.43
CTV.dial CSV @16:51:20.93 CBV.dial :waste2b @16:51:21.82 CS.seek 0ml @16:51:40.53 Disinfecting external Intake with kill @16:51:40.67 CSV.dial CSR @16:51:41.23 Pulling 8ml of CSR.kill @16:51:41.24 CSR.select :kill @16:51:41.38 CS.seek 8ml @16:52:02.85 delay 5 seconds @16:52:07.86 CSR.select :air @16:52:08.00 CS.seek 10ml @16:52:14.06 CSV.dial CBV @16:52:14.09
CBV.dial CSV @16:52:15.29 CTV.dial :intake @16:52:16.15 Intake.select :open @16:52:16.27 CS.seek 2ml @16:52:38.75 Intake.select :close @16:52:38.87 CTV.dial :air @16:52:39.73 CS.reconfigure CSconfig @16:52:39.79 CS.seek 0ml @16:52:45.94 CSV.dial CSR @16:52:47.62 Pulling 5ml of CSR.flush @16:52:47.63 CSR.select :flush @16:52:47.78 CS.seek 5ml @16:53:01.57 delay 5 seconds @16:53:06.58 CSR.select :air @16:53:06.72 CS.seek 10ml @16:53:20.50 CSV.dial CBV @16:53:21.70 CS.seek 0ml @16:53:48.25 Garaging FlushPuck in Collection Clamp @16:53:48.34 Elbow.reconfigure TightElbow @16:53:48.40 Elbow.seek CC @16:53:48.65 Elbow.reconfigure LooseElbow @16:53:48.76 Forearm.seek CC @16:53:49.84 Hand.close @16:53:50.62 CC.seek :open @16:53:55.01 Forearm.seek :retract @16:53:56.07 Elbow.reconfigure TightElbow @16:53:56.12 Elbow.seek :garage @16:53:59.25 Elbow.reconfigure LooseElbow @16:53:59.36 Forearm.reconfigure TightForearm @16:53:59.42 Forearm.seek :garage @16:54:00.60 Hand.open @16:54:01.42 Forearm.reconfigure LooseForearm @16:54:01.48 Forearm.seek :retract @16:54:02.52 Completed [wcrPhase2] begun 15:54:17 @16:54:02.58 Asleep after completing TotalWCRTest assays @16:54:02.59 GoodNews.email "{1 => 1, 2 => 0}\nCan@16:53:32, 23.4C, 44% humidity, 14.5psia, 12.026V, 0.544A, 0.537A avg, 6.54W",Subject: "Asleep after completing TotalWCRTest assays" @16:54:02.62
Mission runtime was 1:00:27.440585 @16:54:08.85 Session Ended @17:09:24.04CEST20-May-19
ESP2 v3.30 I2C Gateway v3.06 for core bus @17:09:26.61 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @17:09:26.87 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @17:09:32.81 Polling Can every 10:00 @17:09:34.60 SPE.coast @17:09:34.69 SPE.configure SPEconfig @17:09:34.86 AS.configure ASconfig @17:09:35.02 SS.reconfigure SSconfig @17:09:35.10 SS.hold @17:09:35.19 SS.configure SSconfig @17:09:35.36 CC.reconfigure CCconfig @17:09:35.40 CC.hold @17:09:35.48 CC.configure CCconfig @17:09:35.56 CS.reconfigure CSconfig @17:09:35.60 CS.hold @17:09:35.66 CS.configure CSconfig @17:09:35.74 PC.reconfigure PCconfig @17:09:35.78 PC.hold @17:09:36.21 PC.configure PCconfig @17:09:36.37 PS.configure PSconfig @17:09:36.56 Forearm.reconfigure LooseForearm @17:09:36.64 Forearm.hold @17:09:36.73 Forearm.configure LooseForearm @17:09:36.88 Elbow.reconfigure LooseElbow @17:09:36.96 Elbow.hold @17:09:37.03 Elbow.configure LooseElbow @17:09:37.13 SC.reconfigure SCloose @17:09:37.17 SC.hold @17:09:37.22 SC.configure SCloose @17:09:37.30 SE.reconfigure SEconfig @17:09:37.34 SE.hold @17:09:37.39 SE.configure SEconfig @17:09:37.43 CH.coast @17:09:37.47 CH.configure CHconfig @17:09:37.50 PH.coast @17:09:37.55 PH.configure PHconfig @17:09:37.58 PCR.off @17:09:37.61 PCR.pidtemp= 50,0.1,0,300,25,25 @17:09:37.65 PCR.caltemp= 2.8814,0.0372,0 @17:09:37.69 All dwarf controllers running firmware version 3.75 @17:09:37.83 Cleanly exited at 17:08:24CEST20-May-19 @17:09:37.89 Loading /home/esp/esp2/mission/TotalWCRTest.rb mission @17:09:37.96 RuntimeError in MAIN -- Mission's startTube should be 3, not 2 @17:09:38.00 Waiting to Resume from Checkpoint "Loading /home/esp/esp2/mission/TotalWCRTest.rb mission" @17:09:38.03 BadNews.email "Mission's startTube should be 3, not 2",Subject: "RuntimeError in MAIN" @17:09:38.33 Session Begun @17:09:44.22 -> showlog 100 @17:10:22.51 -> exit @17:10:22.53 Session Ended @17:10:27.56 Session Begun @17:10:47.94 -> abort! "Rookie mistake" @17:10:47.95 CmdServer::Client::Error in bkha -- Aborting Mission: Rookie mistake @17:10:48.01 BadNews.email "Rookie mistake",Subject: "TotalWCRTest ABORTED!" @17:10:53.68 Session Ended @17:13:28.81CEST20-May-19
>RESET< ESP2 v3.30 I2C Gateway v3.06 for core bus @17:13:31.15 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @17:13:31.61 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @17:13:37.91 Polling Can every 10:00 @17:13:37.95 Loading /home/esp/esp2/mission/TotalWCRTest.rb mission @17:13:38.02 Preparing to sleep until +00:00:10 @17:13:38.03 GoodNews.email "Starting TotalWCRTest w/pucks in tube 2\n{}\nCan@17:13:37, 23.2C, 42% humidity, 14.5psia, 12.144V, 0.096A, 0.096A avg, 1.17W",Subject: "TotalWCRTest begun" @17:13:38.09 Shutdown @17:13:38.12
Asleep til +00:00:10 @17:13:38.14 Continuing at 17:13 @17:13:39.39 Session Begun @17:13:43.90 -> showlog 10 @17:13:44.21 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotalWCRTest.rb:113\n{}\nCan@17:13:38, 23.2C, 42% humidity, 14.5psia, 12.144V, 0.096A, 0.096A avg, 1.17W",Subject: "Asleep until 17:13" @17:13:47.97
Powering core bus @17:13:52.98 Gate.power :raw,:ON @17:13:53.59 SPE.coast @17:13:53.66 SPE.configure SPEconfig @17:13:53.77 AS.configure ASconfig @17:13:53.87 SS.configure SSconfig @17:13:53.98 CC.configure CCconfig @17:13:54.08 CS.configure CSconfig @17:13:54.20 PC.configure PCconfig @17:13:54.30 PS.configure PSconfig @17:13:54.43 Forearm.configure LooseForearm @17:13:54.53 Elbow.configure LooseElbow @17:13:54.66 SC.configure SCloose @17:13:54.76 SE.configure SEconfig @17:13:54.83 CH.coast @17:13:54.89 CH.configure CHconfig @17:13:54.94 PH.coast @17:13:55.00 PH.configure PHconfig @17:13:55.05 PCR.off @17:13:55.09 PCR.pidtemp= 50,0.1,0,300,25,25 @17:13:55.12 PCR.caltemp= 2.8814,0.0372,0 @17:13:55.15 All dwarf controllers running firmware version 3.75 @17:13:55.28 Valve.reset @17:13:55.46 PCR.off @17:13:55.49 PCR.pidtemp= 50,0.1,0,300,25,25 @17:13:55.53 PCR.caltemp= 2.8814,0.0372,0 @17:13:55.69 Intake.select :close @17:13:55.73 Exhaust.select :close @17:13:55.74 CTV.dial :air @17:13:55.87 PSV.dial :air @17:13:55.93 CSV.dial :waste1 @17:13:56.07 PS.reconfigure PSconfig @17:13:56.10
Forearm.reconfigure LooseForearm @17:13:56.20 PS.home.seek 0ml @17:13:56.22
Forearm.home.seek :retract @17:13:56.47 SSV.dial :spit,avoiding: :exhaust @17:13:57.02 Hand.close @17:13:57.05
Elbow.jog 1500 @17:13:57.38 CS.reconfigure CSfast @17:13:57.41 SS.reconfigure SSconfig @17:13:57.51 CS.home.seek 0ml @17:13:57.54 SS.home.seek 0ml @17:13:57.69
Elbow.reconfigure LooseElbow @17:13:57.75 Elbow.home.seek :clear @17:14:01.16 SE.reconfigure SEconfig @17:14:01.22 SE.home.seek :down @17:14:01.90 SC.reconfigure SCsloppy @17:14:01.96 SC.home.seek :initial @17:14:05.50 PS.coast @17:14:12.27
Forearm.seek :retract @17:14:12.36 Elbow.seek :hammer @17:14:13.55 Forearm.reconfigure TightForearm @17:14:13.61 Forearm.seek 5 + 350 counts @17:14:14.72 Forearm.seek :retract @17:14:15.97 PC.reconfigure PCconfig @17:14:15.99
CC.reconfigure CCconfig @17:14:16.07 PC.home.seek :open @17:14:16.11
CC.home.seek :open @17:14:19.66 Phase [wcrPhase2] @17:14:19.69 GoodNews.email "At 17:14:19 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotalWCRTest.rb:113\n{}\nCan@17:14:19, 23.2C, 42% humidity, 14.5psia, 11.972V, 0.708A, 0.708A avg, 8.47W",Subject: "Started TotalWCRTest phase wcrPhase2" @17:14:20.21CEST20-May-19
==================> STARTED WCRSphase (phase #1) <=== @17:14:20.22 Starting stand-alone bac WCR @17:14:20.25 bac wcr sampling at most 2000ml @17:14:20.30 Whole Cell Archive sampling 2000ml @17:14:20.32 Loading wcr puck from tube 2 into Collection Clamp @17:14:20.41 Hand.open @17:14:21.32 SC.reconfigure SCloose @17:14:21.48 Elbow.reconfigure TightElbow @17:14:21.56 Elbow.seek 2 @17:14:22.05 Forearm.seek 2 + 700 counts @17:14:23.13 SE.reconfigure SEpick @17:14:23.22 SE.seek beamBreak @17:14:25.51 BACfans.email "Started stand-alone bac WCR in tube 2 at 17:14:20CEST20-May-19\n{}\nCan@17:14:19, 23.2C, 42% humidity, 14.5psia, 11.972V, 0.708A, 0.708A avg, 8.47W",Subject: "bac wcr sampling at most 2000ml" @17:14:26.36 SE.configure SEconfig @17:14:26.54 Elbow.seek 2 @17:14:26.76 Forearm.seek 2 + 300 counts @17:14:27.02 Forearm.reconfigure LooseForearm @17:14:27.08 Hand.close @17:14:27.88 SE.seek :down @17:14:28.11 Forearm.seek :retract @17:14:29.09 Elbow.seek CC @17:14:30.71 Elbow.reconfigure LooseElbow @17:14:30.82 Forearm.reconfigure TightForearm @17:14:30.90 SE.coast @17:14:30.93 Forearm.seek CC @17:14:32.71 CC.seek :closed @17:14:37.01 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.39 @17:14:37.02 Hand.open @17:14:37.80 Forearm.reconfigure LooseForearm @17:14:37.86 Forearm.seek :retract @17:14:38.93 SC.coast @17:14:38.98 collectSample 2000ml w/bubble point of 55psi @17:14:38.99 Priming 7ml @17:14:39.01 Intake.select :close @17:14:39.03 Exhaust.select :close @17:14:39.06 SS.hold @17:14:39.28 Calibrating pressure and temperature sensors @17:14:39.30 CTV.dial :air @17:14:39.33 SSV.dial :spit,avoiding: :exhaust @17:14:39.37 SS.reconfigure SSfast @17:14:39.44 SS.seek 5ml @17:14:42.32 SSV.dial CBV @17:14:42.35 CBV.dial SSV @17:14:43.87 delay 3.8 seconds @17:14:47.79 SSV.dial :spit @17:14:49.28 SS.seek 0ml @17:14:52.07 SSV.dial CBV @17:14:53.56 CTV.dial :intake @17:14:54.43 SSV.dial CBV @17:14:54.46 CBV.dial SSV @17:14:54.48 Allowing 40s for 7.0ml of remaining 7.0ml @17:14:54.50 Intake.select :open @17:14:56.70 Intake @15.5psia, vacuum from 9.0 to 13.0psi @17:14:56.72 SS.reconfigure SSinhale.with maxSpeed: 150 @17:14:56.79 SS.seek 7ml @17:15:03.18 Stroke completed -- waiting for pressure to equalize ... @17:15:22.04 Intake.select :close @17:15:22.21 SSV.dial :exhaust @17:15:23.06 Exhaust.select :open @17:15:23.19 SS.reconfigure SSexhale @17:15:23.25 SS.seek 0ml @17:15:27.91 Exhaust.select :close @17:15:28.04 Sampling 2000.0ml @17:15:28.05 Intake.select :close @17:15:28.07 Exhaust.select :close @17:15:28.09 SS.reconfigure SSconfig @17:15:28.17 SS.hold @17:15:28.37 CTV.dial :intake @17:15:28.39 SSV.dial CBV @17:15:28.42 CBV.dial SSV @17:15:29.31 Allowing 130s for 25.0ml of remaining 2000.0ml @17:15:29.33 Intake.select :open @17:15:31.52 Intake @15.5psia, vacuum from 9.0 to 13.0psi @17:15:31.54 SS.reconfigure SSinhale.with maxSpeed: 150 @17:15:31.61 SS.seek 25ml @17:15:35.83 Vacuum too high -- waiting up to 40s @17:15:43.96 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:15:43.98 SS.reconfigure SSinhale.with maxSpeed: 135 @17:15:44.06 SS.seek 25ml @17:15:47.71 Vacuum too high -- waiting up to 40s @17:15:56.98 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:15:57.00 SS.reconfigure SSinhale.with maxSpeed: 122 @17:15:57.07 SS.seek 25ml @17:16:00.71 Vacuum too high -- waiting up to 40s @17:16:09.96 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:16:09.98 SS.reconfigure SSinhale.with maxSpeed: 110 @17:16:10.06 SS.seek 25ml @17:16:13.36 Vacuum too high -- waiting up to 40s @17:16:23.10 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:16:23.12 SS.reconfigure SSinhale.with maxSpeed: 99 @17:16:23.20 SS.seek 25ml @17:16:26.92 Vacuum too high -- waiting up to 40s @17:16:38.03 SS.reconfigure SSinhale.with maxSpeed: 89 @17:16:38.10 SS.seek 25ml @17:16:40.62 Stroke completed -- waiting for pressure to equalize ... @17:16:52.63 Intake.select :close @17:16:52.79 SSV.dial :exhaust @17:16:53.68 Exhaust.select :open @17:16:53.81 SS.reconfigure SSexhale @17:16:53.87 SS.seek 0ml @17:17:03.46 Exhaust.select :close @17:17:03.60 SSV.dial CBV @17:17:03.62 CBV.dial SSV @17:17:04.51 Allowing 130s for 25.0ml of remaining 1975.0ml @17:17:04.53 Intake.select :open @17:17:06.72 Intake @15.5psia, vacuum from 9.0 to 13.0psi @17:17:06.74 SS.reconfigure SSinhale.with maxSpeed: 89 @17:17:06.81 SS.seek 25ml @17:17:10.55 Vacuum too high -- waiting up to 40s @17:17:18.07 SS.reconfigure SSinhale.with maxSpeed: 80 @17:17:18.13 SS.seek 25ml @17:17:21.33 Vacuum too high -- waiting up to 40s @17:17:28.75 Intake @15.5psia, vacuum from 9.0 to 13.0psi @17:17:28.77 SS.reconfigure SSinhale.with maxSpeed: 72 @17:17:28.84 SS.seek 25ml @17:17:32.67 Vacuum too high -- waiting up to 40s @17:17:41.03 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:17:41.05 SS.reconfigure SSinhale.with maxSpeed: 65 @17:17:41.12 SS.seek 25ml @17:17:44.92 Vacuum too high -- waiting up to 40s @17:17:53.08 SS.reconfigure SSinhale.with maxSpeed: 59 @17:17:53.14 SS.seek 25ml @17:17:57.27 Vacuum too high -- waiting up to 40s @17:18:06.53 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:18:06.55 SS.reconfigure SSinhale.with maxSpeed: 53 @17:18:06.62 SS.seek 25ml @17:18:11.14 Vacuum too high -- waiting up to 40s @17:18:20.20 SS.reconfigure SSinhale.with maxSpeed: 48 @17:18:20.26 SS.seek 25ml @17:18:25.96 Vacuum too high -- waiting up to 40s @17:18:36.13 Intake @15.5psia, vacuum from 9.0 to 13.0psi @17:18:36.15 SS.reconfigure SSinhale.with maxSpeed: 43 @17:18:36.23 SS.seek 25ml @17:18:41.40 Stroke completed -- waiting for pressure to equalize ... @17:18:54.23 Intake.select :close @17:18:54.39 SSV.dial :exhaust @17:18:55.69 Exhaust.select :open @17:18:55.81 SS.reconfigure SSexhale @17:18:55.87 SS.seek 0ml @17:19:05.46 Exhaust.select :close @17:19:05.59 SSV.dial CBV @17:19:05.61 CBV.dial SSV @17:19:06.49 Allowing 130s for 25.0ml of remaining 1950.0ml @17:19:06.51 Intake.select :open @17:19:08.70 Intake @15.5psia, vacuum from 9.0 to 13.0psi @17:19:08.72 SS.reconfigure SSinhale.with maxSpeed: 43 @17:19:08.79 SS.seek 25ml @17:19:15.58 Vacuum too high -- waiting up to 40s @17:19:22.79 Intake @15.4psia, vacuum from 8.9 to 12.9psi @17:19:22.81 SS.reconfigure SSinhale.with maxSpeed: 39 @17:19:22.89 SS.seek 25ml @17:19:29.49 Vacuum too high -- waiting up to 40s @17:19:37.37 Intake @15.4psia, vacuum from 8.9 to 12.9psi @17:19:37.39 SS.reconfigure SSinhale.with maxSpeed: 35 @17:19:37.46 SS.seek 25ml @17:19:44.99 Vacuum too high -- waiting up to 40s @17:19:53.34 Intake @15.5psia, vacuum from 9.0 to 13.0psi @17:19:53.36 SS.reconfigure SSinhale.with maxSpeed: 32 @17:19:53.44 SS.seek 25ml @17:20:02.80 Vacuum too high -- waiting up to 40s @17:20:11.62 SS.reconfigure SSinhale.with maxSpeed: 29 @17:20:11.69 SS.seek 25ml @17:20:23.61 Vacuum too high -- waiting up to 40s @17:20:33.09 SS.reconfigure SSinhale.with maxSpeed: 26 @17:20:33.16 SS.seek 25ml @17:20:48.96 Stroke completed -- waiting for pressure to equalize ... @17:21:06.03 Intake.select :close @17:21:06.19 SSV.dial :exhaust @17:21:07.07 Exhaust.select :open @17:21:07.20 SS.reconfigure SSexhale @17:21:07.26 SS.seek 0ml @17:21:16.79 Exhaust.select :close @17:21:16.92 SSV.dial CBV @17:21:16.94 CBV.dial SSV @17:21:17.83 Allowing 130s for 25.0ml of remaining 1925.0ml @17:21:17.84 Intake.select :open @17:21:20.03 Intake @15.4psia, vacuum from 8.9 to 12.9psi @17:21:20.05 SS.reconfigure SSinhale.with maxSpeed: 26 @17:21:20.12 SS.seek 25ml @17:21:33.86 Vacuum too high -- waiting up to 40s @17:21:40.81 Intake @15.3psia, vacuum from 8.8 to 12.8psi @17:21:40.83 SS.reconfigure SSinhale.with maxSpeed: 23 @17:21:40.90 SS.seek 25ml @17:21:56.83 Vacuum too high -- waiting up to 40s @17:22:04.74 Intake @15.4psia, vacuum from 8.9 to 12.9psi @17:22:04.76 SS.reconfigure SSinhale.with maxSpeed: 21 @17:22:04.84 SS.seek 25ml @17:22:27.30 Vacuum too high -- waiting up to 40s @17:22:35.86 Intake @15.3psia, vacuum from 8.8 to 12.8psi @17:22:35.88 SS.reconfigure SSinhale.with maxSpeed: 19 @17:22:35.95 SS.seek 25ml @17:23:07.96 Stroke completed -- waiting for pressure to equalize ... @17:23:24.22 Intake.select :close @17:23:24.39 SSV.dial :exhaust @17:23:25.26 Exhaust.select :open @17:23:25.39 SS.reconfigure SSexhale @17:23:25.45 SS.seek 0ml @17:23:34.97 Exhaust.select :close @17:23:35.10 SSV.dial CBV @17:23:35.12 CBV.dial SSV @17:23:36.01 Allowing 130s for 25.0ml of remaining 1900.0ml @17:23:36.03 Intake.select :open @17:23:38.22 Intake @15.4psia, vacuum from 8.9 to 12.9psi @17:23:38.24 SS.reconfigure SSinhale.with maxSpeed: 19 @17:23:38.31 SS.seek 25ml @17:24:06.11 Vacuum too high -- waiting up to 40s @17:24:13.33 Intake @15.3psia, vacuum from 8.8 to 12.8psi @17:24:13.35 SS.reconfigure SSinhale.with maxSpeed: 17 @17:24:13.43 SS.seek 25ml @17:25:11.61 Vacuum too high -- waiting up to 40s @17:25:21.33 SS.reconfigure SSinhale.with maxSpeed: 15 @17:25:21.40 SS.seek 25ml @17:25:39.65 Stroke completed -- waiting for pressure to equalize ... @17:25:46.04 SS.reconfigure SSconfig @17:25:46.10 SS.hold @17:25:46.22 Intake.select :close @17:25:46.38 ShallowSampler::Clogged in WCR -- progress too slow after 125.0ml @17:25:46.39 SSV.dial :exhaust @17:25:47.27 Exhaust.select :open @17:25:47.39 SS.reconfigure SSexhale @17:25:47.46 SS.seek 0ml @17:25:57.10 Exhaust.select :close @17:25:57.23 Sampled 125.0ml @17:25:57.25 Drying puck before fixing for 4:00 @17:25:57.26 CBV.dial :waste2b @17:25:58.17 delay 1:00 @17:26:58.20 CSV.dial :air @17:26:59.05 CS.seek 10ml @17:27:25.92 Pumping wcr puck from tube 2 up to 55psi... @17:27:25.93 CBV.dial SSV @17:27:27.40 CBV.dial between CSV and waste2b @17:27:28.52 CSV.dial between air and CTV @17:27:28.88 CS.seek 7.402ml @17:27:36.51 CTV.dial CSV,avoiding: :air @17:27:36.57 CSV.dial CTV @17:27:37.40 CS.reconfigure CSpressurize @17:27:37.47 CS.seek 0ml @17:27:57.00 CBV.dial :waste2b @17:27:57.37 CS.reconfigure CSconfig @17:27:57.43 CS.seek :empty @17:27:58.39 delay 1:00 @17:28:58.41 CTV.dial :air,avoiding: :intake @17:29:00.40 Fix #1 of 2 (with rnal) for 2:00 @17:29:00.41 CSV.dial CSR @17:29:00.98 Pulling 2ml of CSR.rnal @17:29:00.99 CSR.select :rnal @17:29:01.13 CS.seek 2ml @17:29:07.26 delay 5 seconds @17:29:12.28 CSR.select :air @17:29:12.42 CS.reconfigure CSfast @17:29:12.48 CS.seek 10ml @17:29:34.01 Pumping wcr puck from tube 2 up to 55psi... @17:29:34.03 CBV.dial SSV @17:29:35.46 CBV.dial between CSV and waste2b @17:29:36.57 CSV.dial between air and CTV @17:29:37.27 CS.seek 7.28ml @17:29:45.26 CTV.dial CSV,avoiding: :air @17:29:45.32 CSV.dial CTV @17:29:46.84 CS.reconfigure CSpressurize @17:29:46.91 CS.seek 0ml @17:30:05.13 delay 2:00 @17:32:05.16 CBV.dial :waste2b @17:32:05.55 CS.reconfigure CSconfig @17:32:05.61 CS.seek :empty @17:32:07.70 delay 1:00 @17:33:07.73 CTV.dial :air,avoiding: :intake @17:33:09.27 Fix #2 of 2 (with rnal) for 2:00 @17:33:09.29 CSV.dial CSR @17:33:09.86 Pulling 2ml of CSR.rnal @17:33:09.88 CSR.select :rnal @17:33:10.02 CS.seek 2ml @17:33:16.23 delay 5 seconds @17:33:21.25 CSR.select :air @17:33:21.39 CS.reconfigure CSfast @17:33:21.46 CS.seek 10ml @17:33:42.83 Pumping wcr puck from tube 2 up to 55psi... @17:33:42.84 CBV.dial SSV @17:33:44.28 CBV.dial between CSV and waste2b @17:33:45.37 CSV.dial between air and CTV @17:33:46.07 CS.seek 7.32ml @17:33:53.89 CTV.dial CSV,avoiding: :air @17:33:53.92 CSV.dial CTV @17:33:55.53 CS.reconfigure CSpressurize @17:33:55.59 CS.seek 0ml @17:34:13.79 delay 2:00 @17:36:13.82 CBV.dial :waste2b @17:36:14.20 CS.reconfigure CSconfig @17:36:14.26 CS.seek :empty @17:36:16.39 delay 1:00 @17:37:16.42 CTV.dial :air,avoiding: :intake @17:37:18.01 Storing wcr puck in Collection Clamp to tube 1 @17:37:18.19 SC.reconfigure SCtight @17:37:18.25 SC.seek 1 @17:37:20.39 SC.reconfigure SCloose @17:37:20.61 Elbow.reconfigure TightElbow @17:37:20.67 Elbow.seek 1 @17:37:22.50 Forearm.reconfigure TightForearm @17:37:22.57 Forearm.seek 1 + 700 counts @17:37:23.62 SE.reconfigure SEpick @17:37:23.71 SE.seek beamBreak @17:37:26.76 SE.configure SEconfig @17:37:26.83 SE.seek :place @17:37:27.88 Forearm.reconfigure LooseForearm @17:37:27.95 Forearm.seek :retract @17:37:28.90 Elbow.seek CC @17:37:30.50 Elbow.reconfigure LooseElbow @17:37:30.61 Forearm.seek CC @17:37:31.72 Hand.close @17:37:32.53 CC.seek :open @17:37:37.02 Forearm.seek :retract @17:37:38.12 Elbow.reconfigure TightElbow @17:37:38.19 Elbow.seek 1 @17:37:40.14 Elbow.reconfigure LimpElbow @17:37:40.21 Forearm.seek 1 @17:37:41.26 Elbow.hold @17:37:41.35 Elbow.reconfigure TightElbow @17:37:41.41 Hand.open @17:37:42.25 Forearm.force -20 @17:37:42.31 Forearm.seek 1 + 345 counts @17:37:42.63 Forearm.reconfigure TightForearm @17:37:42.69 Forearm.seek 1 + 700 counts @17:37:43.04 SE.seek :down @17:37:44.16 Forearm.reconfigure LooseForearm @17:37:44.23 Forearm.seek :retract @17:37:45.88 SE.coast @17:37:45.94 SC.coast @17:37:45.99 Starting finalPurge @17:37:46.00 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:37:46.09 Hand.open @17:37:46.51 Elbow.seek FlushPuck::Garage @17:37:48.64 Elbow.reconfigure LooseElbow @17:37:48.74 Forearm.seek :garage @17:37:49.79 Hand.close @17:37:50.62 Forearm.seek :retract @17:37:51.67 Elbow.reconfigure TightElbow @17:37:51.73 Elbow.seek CC @17:37:54.71 Elbow.reconfigure LooseElbow @17:37:54.83 Forearm.reconfigure TightForearm @17:37:54.89 Forearm.seek CC @17:37:56.40 CC.seek :closed @17:38:00.62 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.40 @17:38:00.71 Hand.open @17:38:01.57 Forearm.reconfigure LooseForearm @17:38:01.63 Forearm.seek :retract @17:38:02.70 Flushing the Collection Valve::Manifold @17:38:02.83 CSV.dial CSR @17:38:03.83 Pulling 5ml of CSR.flush @17:38:03.84 CSR.select :flush @17:38:03.99 CS.seek 5ml @17:38:17.80 delay 5 seconds @17:38:22.82 CSR.select :air @17:38:23.00 CS.reconfigure CSfast @17:38:23.06 CS.seek 7.5ml @17:38:30.39 CSV.dial :waste @17:38:31.89 CS.reconfigure CSconfig @17:38:31.95 CS.seek 0ml @17:38:52.11 Flushing lower manifold's waste1 port @17:38:52.25 CSV.dial :flush @17:38:52.76 CS.reconfigure CSfast @17:38:52.82 CS.seek 5ml @17:39:06.49 delay 5 seconds @17:39:11.51 CSV.dial :air @17:39:12.09 CS.seek 7ml @17:39:18.11 CSV.dial CTV @17:39:18.14
CTV.dial CSV @17:39:19.74 CBV.dial :waste1 @17:39:20.62 CS.seek 0ml @17:39:39.30 Flushing lower manifold's waste2b port @17:39:39.44 CSV.dial :flush @17:39:40.31 CS.seek 5ml @17:39:53.89 delay 5 seconds @17:39:58.91 CSV.dial :air @17:39:59.48 CS.seek 7ml @17:40:05.49 CSV.dial CTV @17:40:05.52
CTV.dial CSV @17:40:06.03 CBV.dial :waste2b @17:40:06.90 CS.seek 0ml @17:40:25.55 Flushing upper manifold @17:40:25.68 CSV.dial :flush @17:40:26.56 CS.seek 5ml @17:40:40.30 delay 5 seconds @17:40:45.32 CSV.dial :air @17:40:45.91 CS.seek 7ml @17:40:52.02 CSV.dial CBV @17:40:52.05
CBV.dial CSV @17:40:53.30 CTV.dial :air @17:40:54.84 CS.seek 0ml @17:41:13.55 delay 7 seconds @17:41:20.58 CS.seek 3ml @17:41:29.27 CSV.dial :waste @17:41:29.79 CS.seek 0ml @17:41:38.49 CSV.dial :flush @17:41:39.01 CS.seek 3ml @17:41:47.58 delay 5 seconds @17:41:52.60 CSV.dial :air @17:41:53.17 CS.seek 5ml @17:41:59.30 CSV.dial :air @17:41:59.32 CS.seek 0ml @17:42:13.02 delay 7 seconds @17:42:20.05 CS.seek 3ml @17:42:28.68 CSV.dial :waste @17:42:29.53 CS.seek 0ml @17:42:38.15 Disinfecting external Intake with mfbkill @17:42:38.28 CSV.dial CSR @17:42:39.93 Pulling 8ml of CSR.mfbkill @17:42:39.94 CSR.select :mfbkill @17:42:40.09 CS.seek 8ml @17:43:01.86 delay 5 seconds @17:43:06.88 CSR.select :air @17:43:07.01 CS.seek 10ml @17:43:13.08 CSV.dial CBV @17:43:13.11
CBV.dial CSV @17:43:14.27 CTV.dial :intake @17:43:15.13 Intake.select :open @17:43:15.25 CS.seek 2ml @17:43:37.40 Intake.select :close @17:43:37.51 CTV.dial :air @17:43:38.38 CS.reconfigure CSconfig @17:43:38.44 CS.seek 0ml @17:43:44.52 Flushing lower manifold's waste1 port @17:43:44.53 CSV.dial CSR @17:43:45.79 Pulling 4ml of CSR.mfbkill @17:43:45.80 CSR.select :mfbkill @17:43:45.95 CS.seek 4ml @17:43:57.18 delay 5 seconds @17:44:02.20 CSR.select :air @17:44:02.34 CS.reconfigure CSfast @17:44:02.40 CS.seek 10ml @17:44:18.58 CSV.dial CTV @17:44:18.61
CTV.dial CSV @17:44:20.21 CBV.dial :waste1 @17:44:20.75 CS.seek 0ml @17:44:47.46 Flushing lower manifold's waste2b port @17:44:47.47 CSV.dial CSR @17:44:48.07 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @17:44:48.22 CS.reconfigure CSconfig @17:44:48.28 CS.seek 4ml @17:44:59.46 delay 5 seconds @17:45:04.48 CSR.select :air @17:45:04.62 CS.reconfigure CSfast @17:45:04.69 CS.seek 10ml @17:45:20.87 CSV.dial CTV @17:45:20.90
CTV.dial CSV @17:45:21.44 CBV.dial :waste2b @17:45:22.33 CS.seek 0ml @17:45:48.71 Killing upper manifold @17:45:48.72 CSV.dial CSR @17:45:49.31 Pulling 4ml of CSR.mfbkill @17:45:49.32 CSR.select :mfbkill @17:45:49.47 CS.reconfigure CSconfig @17:45:49.53 CS.seek 4ml @17:46:00.83 delay 5 seconds @17:46:05.85 CSR.select :air @17:46:05.99 CS.reconfigure CSfast @17:46:06.05 CS.seek 10ml @17:46:22.27 CSV.dial CBV @17:46:22.30
CBV.dial CSV @17:46:23.51 CTV.dial :air @17:46:25.05 CS.seek 0ml @17:46:51.33 delay 7 seconds @17:46:58.36 CS.seek 3ml @17:47:06.96 CSV.dial :waste @17:47:07.60 CS.seek 0ml @17:47:16.09 Flushing upper manifold @17:47:16.22 CSV.dial :flush @17:47:16.77 CS.seek 5ml @17:47:30.65 delay 5 seconds @17:47:35.67 CSV.dial :air @17:47:36.24 CS.seek 7ml @17:47:42.30 CSV.dial CBV @17:47:42.33
CBV.dial CSV @17:47:43.52 CTV.dial :air @17:47:43.54 CS.seek 0ml @17:48:02.52 delay 7 seconds @17:48:09.55 CS.seek 3ml @17:48:18.15 CSV.dial :waste @17:48:18.80 CS.seek 0ml @17:48:27.50 CSV.dial :flush @17:48:28.14 CS.seek 3ml @17:48:36.71 delay 5 seconds @17:48:41.73 CSV.dial :air @17:48:42.32 CS.seek 5ml @17:48:48.37 CSV.dial :air @17:48:48.39 CS.seek 0ml @17:49:01.99 delay 7 seconds @17:49:09.02 CS.seek 3ml @17:49:17.68 CSV.dial :waste @17:49:18.55 CS.seek 0ml @17:49:27.18 Flushing lower manifold's waste1 port @17:49:27.31 CSV.dial :flush @17:49:27.85 CS.seek 5ml @17:49:41.49 delay 5 seconds @17:49:46.51 CSV.dial :air @17:49:47.21 CS.seek 7ml @17:49:53.27 CSV.dial CTV @17:49:53.30
CTV.dial CSV @17:49:54.90 CBV.dial :waste1 @17:49:55.42 CS.seek 0ml @17:50:13.93 Flushing lower manifold's waste2b port @17:50:14.06 CSV.dial :flush @17:50:14.92 CS.seek 5ml @17:50:28.52 delay 5 seconds @17:50:33.54 CSV.dial :air @17:50:34.56 CS.seek 7ml @17:50:40.65 CSV.dial CTV @17:50:40.67
CTV.dial CSV @17:50:41.20 CBV.dial :waste2b @17:50:42.08 CS.seek 0ml @17:51:00.59 Disinfecting external Intake with kill @17:51:00.72 CSV.dial CSR @17:51:01.29 Pulling 8ml of CSR.kill CSR.select :kill @17:51:01.44 CS.seek 8ml @17:51:22.71 delay 5 seconds @17:51:27.73 CSR.select :air @17:51:27.87 CS.seek 10ml @17:51:33.96 CSV.dial CBV @17:51:33.99
CBV.dial CSV @17:51:35.20 CTV.dial :intake @17:51:36.04 Intake.select :open @17:51:36.17 CS.seek 2ml @17:51:58.15 Intake.select :close @17:51:58.26 CTV.dial :air @17:51:59.13 CS.reconfigure CSconfig @17:51:59.19 CS.seek 0ml @17:52:05.31 CSV.dial CSR @17:52:06.54 Pulling 5ml of CSR.flush @17:52:06.55 CSR.select :flush @17:52:06.69 CS.seek 5ml @17:52:20.49 delay 5 seconds @17:52:25.51 CSR.select :air @17:52:25.65 CS.seek 10ml @17:52:39.40 CSV.dial CBV @17:52:40.58 CS.seek 0ml @17:53:07.15 Garaging FlushPuck in Collection Clamp @17:53:07.24 Elbow.reconfigure TightElbow @17:53:07.30 Elbow.seek CC @17:53:07.55 Elbow.reconfigure LooseElbow @17:53:07.66 Forearm.seek CC @17:53:08.72 Hand.close @17:53:09.53 CC.seek :open @17:53:13.92 Forearm.seek :retract @17:53:14.97 Elbow.reconfigure TightElbow @17:53:15.03 Elbow.seek :garage @17:53:18.14 Elbow.reconfigure LooseElbow @17:53:18.25 Forearm.reconfigure TightForearm @17:53:18.31 Forearm.seek :garage @17:53:19.47 Hand.open @17:53:20.34 Forearm.reconfigure LooseForearm @17:53:20.40 Forearm.seek :retract @17:53:21.44 Completed [wcrPhase2] begun 17:14:19 @17:53:21.49 Asleep after completing TotalWCRTest assays @17:53:21.51 Mission runtime was 39:43.522018 @17:53:21.53 GoodNews.email "{1 => 1, 2 => 0}\nCan@17:43:40, 23.5C, 43% humidity, 14.5psia, 12.036V, 0.506A, 0.506A avg, 6.09W",Subject: "Asleep after completing TotalWCRTest assays" @17:53:27.83 Session Ended Log::Locked in trapHandler -- Another process is already writing to /var/log/esp/real.log @16:12:02.60CEST21-May-19
>RESET< ESP2 v3.30 I2C Gateway v3.06 for core bus @16:12:05.49 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @16:12:05.93 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @16:12:13.26 Polling Can every 10:00 @16:12:13.36 Loading /home/esp/esp2/mission/TotalWCRTest.rb mission @16:12:13.52 Preparing to sleep until +00:00:10 @16:12:13.58 GoodNews.email "Starting TotalWCRTest w/pucks in tube 2\n{}\nCan@16:12:13, 21.3C, 69% humidity, 14.5psia, 12.124V, 0.178A, 0.178A avg, 2.16W",Subject: "TotalWCRTest begun" @16:12:13.72 Shutdown @16:12:13.76
Asleep til +00:00:10 @16:12:13.80 Continuing at 16:12 @16:12:13.99 Session Begun @16:12:18.23 -> showlog 100 @16:12:20.59 GoodNews.email "Then, in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotalWCRTest.rb:113\n{}\nCan@16:12:13, 21.3C, 69% humidity, 14.5psia, 12.119V, 0.172A, 0.172A avg, 2.08W",Subject: "Asleep until 16:12" @16:12:23.38
Powering core bus @16:12:28.13 Gate.power :raw,:ON @16:12:28.89 SPE.coast @16:12:28.98 SPE.configure SPEconfig @16:12:29.10 AS.configure ASconfig @16:12:29.23 SS.configure SSconfig @16:12:29.35 CC.configure CCconfig @16:12:29.47 CS.configure CSconfig @16:12:29.60 PC.configure PCconfig @16:12:29.74 PS.configure PSconfig @16:12:29.89 Forearm.configure LooseForearm @16:12:30.02 Elbow.configure LooseElbow @16:12:30.16 SC.configure SCloose @16:12:30.29 SE.configure SEconfig @16:12:30.36 CH.coast @16:12:30.43 CH.configure CHconfig @16:12:30.49 PH.coast @16:12:30.58 PH.configure PHconfig @16:12:30.64 PCR.off @16:12:30.70 PCR.pidtemp= 50,0.1,0,300,25,25 @16:12:30.75 PCR.caltemp= 2.8814,0.0372,0 @16:12:30.80 All dwarf controllers running firmware version 3.75 @16:12:30.93 Valve.reset @16:12:31.13 PCR.off @16:12:31.20 PCR.pidtemp= 50,0.1,0,300,25,25 @16:12:31.26 PCR.caltemp= 2.8814,0.0372,0 @16:12:31.48 Intake.select :close @16:12:31.52 Exhaust.select :close @16:12:31.63 CTV.dial :air @16:12:31.76 PSV.dial :air @16:12:31.83 CSV.dial :waste1 @16:12:32.16 SSV.dial :spit,avoiding: :exhaust @16:12:32.21
Forearm.reconfigure LooseForearm @16:12:32.24 PS.reconfigure PSconfig @16:12:32.55 PS.home.seek 0ml @16:12:32.67
Forearm.home.seek :retract @16:12:33.03 CS.reconfigure CSfast @16:12:33.11 SS.reconfigure SSconfig @16:12:33.25 CS.home.seek 0ml @16:12:33.30 SS.home.seek 0ml @16:12:33.51 Hand.close @16:12:33.55
Elbow.jog 1500 @16:12:34.12 Elbow.reconfigure LooseElbow @16:12:34.19 Elbow.home.seek :clear @16:12:38.87 SE.reconfigure SEconfig @16:12:38.94 SE.home.seek :down @16:12:39.77 SC.reconfigure SCsloppy @16:12:39.84 SC.home.seek :initial @16:12:41.87 PS.coast @16:12:50.14
Forearm.seek :retract @16:12:50.24 Elbow.seek :hammer @16:12:51.45 Forearm.reconfigure TightForearm @16:12:51.51 Forearm.seek 5 + 350 counts @16:12:52.62 Forearm.seek :retract @16:12:53.86 PC.reconfigure PCconfig @16:12:53.88
CC.reconfigure CCconfig @16:12:54.00 CC.home.seek :open @16:12:54.03 PC.home.seek :open @16:12:57.66 GoodNews.email "At 16:12:57 began in tube 2: wcrPhase2\n/home/esp/esp2/mission/TotalWCRTest.rb:113\n{}\nCan@16:12:57, 21.3C, 68% humidity, 14.5psia, 11.957V, 0.802A, 0.796A avg, 9.59W",Subject: "Started TotalWCRTest phase wcrPhase2" @16:12:57.68
Phase [wcrPhase2] @16:12:58.32CEST21-May-19 ==================> STARTED WCRSphase (phase #1) <=== @16:12:58.33 Starting initialPurge @16:12:58.36 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:12:58.46 Hand.open @16:12:59.40 Elbow.reconfigure TightElbow @16:12:59.48 Elbow.seek FlushPuck::Garage @16:13:01.50 Elbow.reconfigure LooseElbow @16:13:01.66 Forearm.reconfigure LooseForearm @16:13:01.78 Forearm.seek :garage @16:13:02.90 Hand.close @16:13:03.78 Forearm.seek :retract @16:13:04.85 Elbow.reconfigure TightElbow @16:13:04.92 Elbow.seek CC @16:13:07.78 Elbow.reconfigure LooseElbow @16:13:07.91 Forearm.reconfigure TightForearm @16:13:07.97 Forearm.seek CC @16:13:09.34 CC.seek :closed @16:13:13.61 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.50 @16:13:13.70 Hand.open @16:13:14.48 Forearm.reconfigure LooseForearm @16:13:14.55 Forearm.seek :retract @16:13:15.62 Flushing the Collection Valve::Manifold @16:13:15.77 CSV.dial CSR @16:13:17.35 Pulling 5ml of CSR.flush @16:13:17.36 CSR.select :flush @16:13:17.52 CS.reconfigure CSconfig @16:13:17.59 CS.seek 5ml @16:13:31.57 delay 5 seconds @16:13:36.60 CSR.select :air @16:13:36.80 CS.reconfigure CSfast @16:13:36.86 CS.seek 7.5ml @16:13:44.41 CSV.dial :waste @16:13:45.93 CS.reconfigure CSconfig @16:13:46.01 CS.seek 0ml @16:14:06.54 Purging external Intake @16:14:06.69 CSV.dial :flush @16:14:07.23 CS.reconfigure CSfast @16:14:07.30 CS.seek 10ml @16:14:34.35 delay 5 seconds @16:14:39.37 CSV.dial CBV @16:14:39.41
CBV.dial CSV @16:14:40.30 CTV.dial :intake @16:14:41.18 Intake.select :open @16:14:41.32 CS.seek 0ml @16:15:09.41 Intake.select :close @16:15:09.54 CTV.dial :air @16:15:10.43 Flushing upper manifold @16:15:10.58 CSV.dial :flush @16:15:11.44 CS.seek 5ml @16:15:25.29 delay 5 seconds @16:15:30.32 CSV.dial :air @16:15:31.06 CS.seek 7ml @16:15:37.26 CSV.dial CBV @16:15:37.29
CBV.dial CSV @16:15:38.49 CTV.dial :air @16:15:38.52 CS.seek 0ml @16:15:57.63 delay 7 seconds @16:16:04.67 CS.seek 3ml @16:16:14.04 CSV.dial :waste @16:16:14.71 CS.seek 0ml @16:16:23.50 CSV.dial :flush @16:16:24.05 CS.seek 3ml @16:16:32.85 delay 5 seconds @16:16:37.88 CSV.dial :air @16:16:38.47 CS.seek 5ml @16:16:44.63 CSV.dial :air @16:16:44.66 CS.seek 0ml @16:16:58.54 delay 7 seconds @16:17:05.58 CS.seek 3ml @16:17:14.38 CSV.dial :waste @16:17:15.25 CS.seek 0ml @16:17:23.95 Garaging FlushPuck in Collection Clamp @16:17:24.05 Elbow.reconfigure TightElbow @16:17:24.12 Elbow.seek CC @16:17:24.41 Elbow.reconfigure LooseElbow @16:17:24.53 Forearm.seek CC @16:17:25.62 Hand.close @16:17:26.41 CC.seek :open @16:17:30.83 Forearm.seek :retract @16:17:31.85 Elbow.reconfigure TightElbow @16:17:31.92 Elbow.seek :garage @16:17:35.15 Elbow.reconfigure LooseElbow @16:17:35.27 Forearm.reconfigure TightForearm @16:17:35.34 Forearm.seek :garage @16:17:36.54 Hand.open @16:17:37.35 Forearm.reconfigure LooseForearm @16:17:37.42 Forearm.seek :retract @16:17:38.53 Starting stand-alone bac WCR @16:17:38.54 bac wcr sampling at most 2000ml @16:17:38.60 Whole Cell Archive sampling 2000ml @16:17:38.62 BACfans.email "Started stand-alone bac WCR in tube 2 at 16:17:38CEST21-May-19\n{}\nCan@16:12:57, 21.3C, 68% humidity, 14.5psia, 11.957V, 0.802A, 0.796A avg, 9.59W",Subject: "bac wcr sampling at most 2000ml" @16:17:38.67 Loading wcr puck from tube 2 into Collection Clamp @16:17:39.05 SC.reconfigure SCloose @16:17:39.32 Elbow.reconfigure TightElbow @16:17:39.40 Elbow.seek 2 @16:17:41.56 Forearm.reconfigure TightForearm @16:17:41.64 Forearm.seek 2 + 700 counts @16:17:42.83 SE.reconfigure SEpick @16:17:42.98 SE.seek beamBreak @16:17:46.35 SE.configure SEconfig @16:17:46.56 Elbow.seek 2 @16:17:46.79 Forearm.seek 2 + 300 counts @16:17:47.19 Forearm.reconfigure LooseForearm @16:17:47.26 Hand.close @16:17:48.05 SE.seek :down @16:17:48.29 Forearm.seek :retract @16:17:49.69 Elbow.seek CC @16:17:51.11 SE.coast @16:17:51.36 Elbow.reconfigure LooseElbow @16:17:51.51 Forearm.reconfigure TightForearm @16:17:51.60 Forearm.seek CC @16:17:53.01 CC.seek :closed @16:17:57.38 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.32 @16:17:57.39 Hand.open @16:17:58.22 Forearm.reconfigure LooseForearm @16:17:58.29 Forearm.seek :retract @16:17:59.36 SC.coast @16:17:59.42 collectSample 2000ml w/bubble point of 55psi @16:17:59.44 Priming 7ml @16:17:59.47 Intake.select :close @16:17:59.49 Exhaust.select :close @16:17:59.53 SS.hold @16:17:59.80 Calibrating pressure and temperature sensors @16:17:59.82 CTV.dial :air @16:17:59.85 SSV.dial :spit,avoiding: :exhaust @16:17:59.90 SS.reconfigure SSfast @16:17:59.98 SS.seek 5ml @16:18:02.81 SSV.dial CBV @16:18:02.85 CBV.dial SSV @16:18:04.41 delay 3.8 seconds @16:18:08.34 SSV.dial :spit @16:18:09.88 SS.seek 0ml @16:18:12.59 SSV.dial CBV @16:18:14.11 CTV.dial :intake @16:18:14.99 SSV.dial CBV @16:18:15.03 CBV.dial SSV @16:18:15.06 Allowing 40s for 7.0ml of remaining 7.0ml @16:18:15.09 Intake.select :open @16:18:17.29 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:18:17.32 SS.reconfigure SSinhale.with maxSpeed: 150 @16:18:17.41 SS.seek 7ml @16:18:22.88 Stroke completed -- waiting for pressure to equalize ... @16:18:36.01 Intake.select :close @16:18:36.20 SSV.dial :exhaust @16:18:37.09 Exhaust.select :open @16:18:37.23 SS.reconfigure SSexhale @16:18:37.30 SS.seek 0ml @16:18:41.43 Exhaust.select :close @16:18:41.56 Sampling 2000.0ml @16:18:41.57 Intake.select :close @16:18:41.59 Exhaust.select :close @16:18:41.63 SS.reconfigure SSconfig @16:18:41.71 SS.hold @16:18:42.45 CTV.dial :intake @16:18:42.48 SSV.dial CBV @16:18:42.51 CBV.dial SSV @16:18:43.42 Allowing 130s for 25.0ml of remaining 2000.0ml @16:18:43.44 Intake.select :open @16:18:45.66 Intake @15.4psia, vacuum from 8.9 to 12.9psi @16:18:45.68 SS.reconfigure SSinhale.with maxSpeed: 150 @16:18:45.77 SS.seek 25ml @16:18:50.59 Vacuum too high -- waiting up to 40s @16:19:00.68 Intake @14.7psia, vacuum from 8.2 to 12.2psi @16:19:00.71 SS.reconfigure SSinhale.with maxSpeed: 135 @16:19:00.80 SS.seek 25ml @16:19:05.34 Vacuum too high -- waiting up to 40s @16:19:20.19 Intake @14.8psia, vacuum from 8.3 to 12.3psi @16:19:20.21 SS.reconfigure SSinhale.with maxSpeed: 122 @16:19:20.30 SS.seek 25ml @16:19:25.85 Vacuum too high -- waiting up to 40s @16:19:42.18 Intake @15.0psia, vacuum from 8.5 to 12.5psi @16:19:42.20 SS.reconfigure SSinhale.with maxSpeed: 110 @16:19:42.29 SS.seek 25ml @16:19:46.95 Stroke completed -- waiting for pressure to equalize ... @16:20:01.88 Intake.select :close @16:20:02.06 SSV.dial :exhaust @16:20:02.94 Exhaust.select :open @16:20:03.09 SS.reconfigure SSexhale @16:20:03.16 SS.seek 0ml @16:20:12.65 Exhaust.select :close @16:20:12.79 SSV.dial CBV @16:20:12.82 CBV.dial SSV @16:20:13.73 Allowing 130s for 25.0ml of remaining 1975.0ml @16:20:13.75 Intake.select :open @16:20:15.97 Intake @15.3psia, vacuum from 8.8 to 12.8psi @16:20:15.99 SS.reconfigure SSinhale.with maxSpeed: 110 @16:20:16.08 SS.seek 25ml @16:20:20.50 Vacuum too high -- waiting up to 40s @16:20:28.82 Intake @14.9psia, vacuum from 8.4 to 12.4psi @16:20:29.27 SS.reconfigure SSinhale.with maxSpeed: 99 @16:20:29.45 SS.seek 25ml @16:20:33.62 Vacuum too high -- waiting up to 40s @16:20:42.70 Intake @15.0psia, vacuum from 8.5 to 12.5psi @16:20:42.73 SS.reconfigure SSinhale.with maxSpeed: 89 @16:20:42.82 SS.seek 25ml @16:20:47.81 Vacuum too high -- waiting up to 40s @16:20:58.67 SS.reconfigure SSinhale.with maxSpeed: 80 @16:20:58.75 SS.seek 25ml @16:21:05.53 Vacuum too high -- waiting up to 40s @16:21:18.18 Intake @15.1psia, vacuum from 8.6 to 12.6psi @16:21:18.21 SS.reconfigure SSinhale.with maxSpeed: 72 @16:21:18.30 SS.seek 25ml @16:21:22.23 Stroke completed -- waiting for pressure to equalize ... @16:21:32.29 Intake.select :close @16:21:32.47 SSV.dial :exhaust @16:21:33.37 Exhaust.select :open @16:21:33.51 SS.reconfigure SSexhale @16:21:33.58 SS.seek 0ml @16:21:43.24 Exhaust.select :close @16:21:43.39 SSV.dial CBV @16:21:43.42 CBV.dial SSV @16:21:44.32 Allowing 130s for 25.0ml of remaining 1950.0ml @16:21:44.34 Intake.select :open @16:21:46.55 Intake @15.3psia, vacuum from 8.8 to 12.8psi @16:21:46.58 SS.reconfigure SSinhale.with maxSpeed: 72 @16:21:46.67 SS.seek 25ml @16:21:52.03 Vacuum too high -- waiting up to 40s @16:21:58.90 Intake @15.0psia, vacuum from 8.5 to 12.5psi @16:21:58.93 SS.reconfigure SSinhale.with maxSpeed: 65 @16:21:59.02 SS.seek 25ml @16:22:04.84 Vacuum too high -- waiting up to 40s @16:22:12.94 Intake @14.9psia, vacuum from 8.4 to 12.4psi @16:22:12.96 SS.reconfigure SSinhale.with maxSpeed: 59 @16:22:13.05 SS.seek 25ml @16:22:20.72 Vacuum too high -- waiting up to 40s @16:22:30.32 Intake @15.0psia, vacuum from 8.5 to 12.5psi @16:22:30.35 SS.reconfigure SSinhale.with maxSpeed: 53 @16:22:30.44 SS.seek 25ml @16:22:40.97 Vacuum too high -- waiting up to 40s @16:22:52.07 Intake @15.0psia, vacuum from 8.5 to 12.5psi @16:22:52.09 SS.reconfigure SSinhale.with maxSpeed: 48 @16:22:52.18 SS.seek 25ml @16:22:55.99 Stroke completed -- waiting for pressure to equalize ... @16:23:03.56 Intake.select :close @16:23:03.74 SSV.dial :exhaust @16:23:04.63 Exhaust.select :open @16:23:04.77 SS.reconfigure SSexhale @16:23:04.84 SS.seek 0ml @16:23:14.48 Exhaust.select :close @16:23:14.62 SSV.dial CBV @16:23:14.65 CBV.dial SSV @16:23:15.55 Allowing 130s for 25.0ml of remaining 1925.0ml @16:23:15.57 Intake.select :open @16:23:17.79 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:23:17.81 SS.reconfigure SSinhale.with maxSpeed: 48 @16:23:17.91 SS.seek 25ml @16:23:26.68 Vacuum too high -- waiting up to 40s @16:23:33.01 Intake @15.0psia, vacuum from 8.5 to 12.5psi @16:23:33.03 SS.reconfigure SSinhale.with maxSpeed: 43 @16:23:33.12 SS.seek 25ml @16:23:45.09 Vacuum too high -- waiting up to 40s @16:23:53.73 SS.reconfigure SSinhale.with maxSpeed: 39 @16:23:53.81 SS.seek 25ml @16:24:11.09 Vacuum too high -- waiting up to 40s @16:24:20.94 Intake @15.0psia, vacuum from 8.5 to 12.5psi @16:24:20.97 SS.reconfigure SSinhale.with maxSpeed: 35 @16:24:21.07 SS.seek 25ml @16:24:28.35 Stroke completed -- waiting for pressure to equalize ... @16:24:35.91 Intake.select :close @16:24:36.09 SSV.dial :exhaust @16:24:36.96 Exhaust.select :open @16:24:37.10 SS.reconfigure SSexhale @16:24:37.17 SS.seek 0ml @16:24:46.77 Exhaust.select :close @16:24:46.92 SSV.dial CBV @16:24:46.95 CBV.dial SSV @16:24:47.85 Allowing 130s for 25.0ml of remaining 1900.0ml @16:24:47.87 Intake.select :open @16:24:50.08 Intake @15.3psia, vacuum from 8.8 to 12.8psi @16:24:50.11 SS.reconfigure SSinhale.with maxSpeed: 35 @16:24:50.19 SS.seek 25ml @16:25:09.31 Vacuum too high -- waiting up to 40s @16:25:16.40 Intake @14.9psia, vacuum from 8.4 to 12.4psi @16:25:16.42 SS.reconfigure SSinhale.with maxSpeed: 32 @16:25:16.51 SS.seek 25ml @16:25:40.18 Vacuum too high -- waiting up to 40s @16:25:48.57 Intake @15.1psia, vacuum from 8.6 to 12.6psi @16:25:48.59 SS.reconfigure SSinhale.with maxSpeed: 29 @16:25:48.68 SS.seek 25ml @16:26:00.96 Stroke completed -- waiting for pressure to equalize ... @16:26:09.13 Intake.select :close @16:26:09.31 SSV.dial :exhaust @16:26:10.19 Exhaust.select :open @16:26:10.34 SS.reconfigure SSexhale @16:26:10.41 SS.seek 0ml @16:26:20.18 Exhaust.select :close @16:26:20.33 SSV.dial CBV @16:26:20.36 CBV.dial SSV @16:26:21.26 Allowing 130s for 25.0ml of remaining 1875.0ml @16:26:21.28 Intake.select :open @16:26:23.49 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:26:23.52 SS.reconfigure SSinhale.with maxSpeed: 29 @16:26:23.62 SS.seek 25ml @16:27:05.28 Vacuum too high -- waiting up to 40s @16:27:12.63 Intake @15.0psia, vacuum from 8.5 to 12.5psi @16:27:12.66 SS.reconfigure SSinhale.with maxSpeed: 26 @16:27:12.76 SS.seek 25ml @16:27:34.54 Stroke completed -- waiting for pressure to equalize ... @16:27:43.34 Intake.select :close @16:27:43.52 SSV.dial :exhaust @16:27:44.41 Exhaust.select :open @16:27:44.55 SS.reconfigure SSexhale @16:27:44.63 SS.seek 0ml @16:27:54.44 Exhaust.select :close @16:27:54.59 SSV.dial CBV @16:27:54.62 CBV.dial SSV @16:27:55.52 Allowing 130s for 25.0ml of remaining 1850.0ml @16:27:55.54 Intake.select :open @16:27:57.75 Intake @15.4psia, vacuum from 8.9 to 12.9psi @16:27:57.78 SS.reconfigure SSinhale.with maxSpeed: 26 @16:27:57.86 SS.seek 25ml @16:29:06.07 Stroke completed -- waiting for pressure to equalize ... @16:29:17.25 Intake.select :close @16:29:17.44 SSV.dial :exhaust @16:29:18.32 Exhaust.select :open @16:29:18.47 SS.reconfigure SSexhale @16:29:18.54 SS.seek 0ml @16:29:28.43 Exhaust.select :close @16:29:28.58 SSV.dial CBV @16:29:28.61 CBV.dial SSV @16:29:29.51 Allowing 130s for 25.0ml of remaining 1825.0ml @16:29:29.53 Intake.select :open @16:29:31.73 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:29:31.76 SS.reconfigure SSinhale.with maxSpeed: 26 @16:29:31.86 SS.seek 25ml @16:30:40.15 Stroke completed -- waiting for pressure to equalize ... @16:30:50.81 Intake.select :close @16:30:50.98 SSV.dial :exhaust @16:30:51.86 Exhaust.select :open @16:30:52.00 SS.reconfigure SSexhale @16:30:52.07 SS.seek 0ml @16:31:01.66 Exhaust.select :close @16:31:01.81 SSV.dial CBV @16:31:01.84 CBV.dial SSV @16:31:02.74 Allowing 130s for 25.0ml of remaining 1800.0ml @16:31:02.76 Intake.select :open @16:31:04.97 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:31:05.00 SS.reconfigure SSinhale.with maxSpeed: 26 @16:31:05.10 SS.seek 25ml @16:32:12.74 Stroke completed -- waiting for pressure to equalize ... @16:32:24.05 Intake.select :close @16:32:24.23 SSV.dial :exhaust @16:32:25.11 Exhaust.select :open @16:32:25.26 SS.reconfigure SSexhale @16:32:25.33 SS.seek 0ml @16:32:34.58 Exhaust.select :close @16:32:34.72 SSV.dial CBV @16:32:34.75 CBV.dial SSV @16:32:35.64 Allowing 130s for 25.0ml of remaining 1775.0ml @16:32:35.66 Intake.select :open @16:32:37.87 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:32:37.90 SS.reconfigure SSinhale.with maxSpeed: 26 @16:32:38.50 SS.seek 25ml @16:33:46.68 Stroke completed -- waiting for pressure to equalize ... @16:33:58.60 Intake.select :close @16:33:58.79 SSV.dial :exhaust @16:33:59.66 Exhaust.select :open @16:33:59.81 SS.reconfigure SSexhale @16:33:59.88 SS.seek 0ml @16:34:09.44 Exhaust.select :close @16:34:09.59 SSV.dial CBV @16:34:09.62 CBV.dial SSV @16:34:10.52 Allowing 130s for 25.0ml of remaining 1750.0ml @16:34:10.54 Intake.select :open @16:34:12.75 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:34:12.78 SS.reconfigure SSinhale.with maxSpeed: 26 @16:34:12.87 SS.seek 25ml @16:35:20.43 Stroke completed -- waiting for pressure to equalize ... @16:35:32.37 Intake.select :close @16:35:32.55 SSV.dial :exhaust @16:35:33.44 Exhaust.select :open @16:35:33.58 SS.reconfigure SSexhale @16:35:33.65 SS.seek 0ml @16:35:43.57 Exhaust.select :close @16:35:43.72 SSV.dial CBV @16:35:43.75 CBV.dial SSV @16:35:44.66 Allowing 130s for 25.0ml of remaining 1725.0ml @16:35:44.68 Intake.select :open @16:35:46.89 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:35:46.92 SS.reconfigure SSinhale.with maxSpeed: 26 @16:35:47.02 SS.seek 25ml @16:36:54.94 Stroke completed -- waiting for pressure to equalize ... @16:37:06.15 Intake.select :close @16:37:06.34 SSV.dial :exhaust @16:37:07.24 Exhaust.select :open @16:37:07.38 SS.reconfigure SSexhale @16:37:07.45 SS.seek 0ml @16:37:17.19 Exhaust.select :close @16:37:17.33 SSV.dial CBV @16:37:17.36 CBV.dial SSV @16:37:18.25 Allowing 130s for 25.0ml of remaining 1700.0ml @16:37:18.27 Intake.select :open @16:37:20.48 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:37:20.50 SS.reconfigure SSinhale.with maxSpeed: 26 @16:37:20.60 SS.seek 25ml @16:38:28.42 Stroke completed -- waiting for pressure to equalize ... @16:38:39.11 Intake.select :close @16:38:39.29 SSV.dial :exhaust @16:38:40.16 Exhaust.select :open @16:38:40.31 SS.reconfigure SSexhale @16:38:40.38 SS.seek 0ml @16:38:50.13 Exhaust.select :close @16:38:50.27 SSV.dial CBV @16:38:50.31 CBV.dial SSV @16:38:51.21 Allowing 130s for 25.0ml of remaining 1675.0ml @16:38:51.23 Intake.select :open @16:38:53.45 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:38:53.47 SS.reconfigure SSinhale.with maxSpeed: 26 @16:38:53.56 SS.seek 25ml @16:40:01.14 Stroke completed -- waiting for pressure to equalize ... @16:40:12.92 Intake.select :close @16:40:13.11 SSV.dial :exhaust @16:40:14.00 Exhaust.select :open @16:40:14.15 SS.reconfigure SSexhale @16:40:14.22 SS.seek 0ml @16:40:23.89 Exhaust.select :close @16:40:24.04 SSV.dial CBV @16:40:24.07 CBV.dial SSV @16:40:24.97 Allowing 130s for 25.0ml of remaining 1650.0ml @16:40:24.99 Intake.select :open @16:40:27.21 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:40:27.23 SS.reconfigure SSinhale.with maxSpeed: 26 @16:40:27.33 SS.seek 25ml @16:41:34.89 Stroke completed -- waiting for pressure to equalize ... @16:41:46.14 Intake.select :close @16:41:46.32 SSV.dial :exhaust @16:41:47.20 Exhaust.select :open @16:41:47.34 SS.reconfigure SSexhale @16:41:47.41 SS.seek 0ml @16:41:57.05 Exhaust.select :close @16:41:57.19 SSV.dial CBV @16:41:57.22 CBV.dial SSV @16:41:58.13 Allowing 130s for 25.0ml of remaining 1625.0ml @16:41:58.15 Intake.select :open @16:42:00.35 Intake @15.5psia, vacuum from 9.0 to 13.0psi @16:42:00.38 SS.reconfigure SSinhale.with maxSpeed: 26 @16:42:00.47 SS.seek 25ml @16:43:08.19 Stroke completed -- waiting for pressure to equalize ... @16:43:19.47 Intake.select :close @16:43:20.06 SSV.dial :exhaust @16:43:21.03 Exhaust.select :open @16:43:21.18 SS.reconfigure SSexhale @16:43:21.25 SS.seek 0ml @16:43:30.92 Exhaust.select :close @16:43:31.07 SSV.dial CBV @16:43:31.10 CBV.dial SSV @16:43:32.00 Allowing 130s for 25.0ml of remaining 1600.0ml @16:43:32.03 Intake.select :open @16:43:34.23 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:43:34.26 SS.reconfigure SSinhale.with maxSpeed: 26 @16:43:34.35 SS.seek 25ml @16:44:27.71 Vacuum too high -- waiting up to 40s @16:44:33.54 Intake @15.2psia, vacuum from 8.7 to 12.7psi @16:44:33.57 SS.reconfigure SSinhale.with maxSpeed: 23 @16:44:33.67 SS.seek 25ml @16:44:50.53 Stroke completed -- waiting for pressure to equalize ... @16:44:57.44 Intake.select :close @16:44:57.63 SSV.dial :exhaust @16:44:58.52 Exhaust.select :open @16:44:58.66 SS.reconfigure SSexhale @16:44:58.73 SS.seek 0ml @16:45:08.28 Exhaust.select :close @16:45:08.42 SSV.dial CBV @16:45:08.45 CBV.dial SSV @16:45:09.34 Allowing 130s for 25.0ml of remaining 1575.0ml @16:45:09.36 Intake.select :open @16:45:11.57 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:45:11.60 SS.reconfigure SSinhale.with maxSpeed: 23 @16:45:11.70 SS.seek 25ml @16:46:28.50 Stroke completed -- waiting for pressure to equalize ... @16:46:37.21 Intake.select :close @16:46:37.39 SSV.dial :exhaust @16:46:38.28 Exhaust.select :open @16:46:38.43 SS.reconfigure SSexhale @16:46:38.50 SS.seek 0ml @16:46:48.08 Exhaust.select :close @16:46:48.22 SSV.dial CBV @16:46:48.25 CBV.dial SSV @16:46:49.16 Allowing 130s for 25.0ml of remaining 1550.0ml @16:46:49.18 Intake.select :open @16:46:51.39 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:46:51.41 SS.reconfigure SSinhale.with maxSpeed: 23 @16:46:51.50 SS.seek 25ml @16:48:08.08 Stroke completed -- waiting for pressure to equalize ... @16:48:16.86 Intake.select :close @16:48:17.05 SSV.dial :exhaust @16:48:17.92 Exhaust.select :open @16:48:18.07 SS.reconfigure SSexhale @16:48:18.14 SS.seek 0ml @16:48:27.73 Exhaust.select :close @16:48:27.87 SSV.dial CBV @16:48:27.91 CBV.dial SSV @16:48:28.80 Allowing 130s for 25.0ml of remaining 1525.0ml @16:48:28.83 Intake.select :open @16:48:31.02 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:48:31.04 SS.reconfigure SSinhale.with maxSpeed: 23 @16:48:31.13 SS.seek 25ml @16:49:47.61 Stroke completed -- waiting for pressure to equalize ... @16:49:56.39 Intake.select :close @16:49:56.57 SSV.dial :exhaust @16:49:57.95 Exhaust.select :open @16:49:58.10 SS.reconfigure SSexhale @16:49:58.18 SS.seek 0ml @16:50:07.44 Exhaust.select :close @16:50:07.59 SSV.dial CBV @16:50:07.62 CBV.dial SSV @16:50:08.53 Allowing 130s for 25.0ml of remaining 1500.0ml @16:50:08.55 Intake.select :open @16:50:10.78 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:50:10.81 SS.reconfigure SSinhale.with maxSpeed: 23 @16:50:10.89 SS.seek 25ml @16:51:27.61 Stroke completed -- waiting for pressure to equalize ... @16:51:36.40 Intake.select :close @16:51:36.58 SSV.dial :exhaust @16:51:37.47 Exhaust.select :open @16:51:37.62 SS.reconfigure SSexhale @16:51:37.69 SS.seek 0ml @16:51:47.34 Exhaust.select :close @16:51:47.49 SSV.dial CBV @16:51:47.52 CBV.dial SSV @16:51:48.42 Allowing 130s for 25.0ml of remaining 1475.0ml @16:51:48.44 Intake.select :open @16:51:50.65 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:51:50.68 SS.reconfigure SSinhale.with maxSpeed: 23 @16:51:50.77 SS.seek 25ml @16:53:07.20 Stroke completed -- waiting for pressure to equalize ... @16:53:16.02 Intake.select :close @16:53:16.20 SSV.dial :exhaust @16:53:17.08 Exhaust.select :open @16:53:17.23 SS.reconfigure SSexhale @16:53:17.31 SS.seek 0ml @16:53:26.89 Exhaust.select :close @16:53:27.03 SSV.dial CBV @16:53:27.06 CBV.dial SSV @16:53:27.97 Allowing 130s for 25.0ml of remaining 1450.0ml @16:53:28.00 Intake.select :open @16:53:30.20 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:53:30.23 SS.reconfigure SSinhale.with maxSpeed: 23 @16:53:30.33 SS.seek 25ml @16:54:47.00 Stroke completed -- waiting for pressure to equalize ... @16:54:56.31 Intake.select :close @16:54:56.49 SSV.dial :exhaust @16:54:57.39 Exhaust.select :open @16:54:57.55 SS.reconfigure SSexhale @16:54:57.62 SS.seek 0ml @16:55:06.86 Exhaust.select :close @16:55:06.99 SSV.dial CBV @16:55:07.02 CBV.dial SSV @16:55:07.92 Allowing 130s for 25.0ml of remaining 1425.0ml @16:55:07.94 Intake.select :open @16:55:10.15 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:55:10.18 SS.reconfigure SSinhale.with maxSpeed: 23 @16:55:10.28 SS.seek 25ml @16:56:26.92 Stroke completed -- waiting for pressure to equalize ... @16:56:36.35 Intake.select :close @16:56:36.53 SSV.dial :exhaust @16:56:37.42 Exhaust.select :open @16:56:37.61 SS.reconfigure SSexhale @16:56:37.68 SS.seek 0ml @16:56:47.32 Exhaust.select :close @16:56:47.46 SSV.dial CBV @16:56:47.49 CBV.dial SSV @16:56:48.39 Allowing 130s for 25.0ml of remaining 1400.0ml @16:56:48.41 Intake.select :open @16:56:50.62 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:56:50.65 SS.reconfigure SSinhale.with maxSpeed: 23 @16:56:50.74 SS.seek 25ml @16:58:07.18 Stroke completed -- waiting for pressure to equalize ... @16:58:16.46 Intake.select :close @16:58:16.63 SSV.dial :exhaust @16:58:17.51 Exhaust.select :open @16:58:17.66 SS.reconfigure SSexhale @16:58:17.74 SS.seek 0ml @16:58:27.31 Exhaust.select :close @16:58:27.46 SSV.dial CBV @16:58:27.49 CBV.dial SSV @16:58:28.39 Allowing 130s for 25.0ml of remaining 1375.0ml @16:58:28.41 Intake.select :open @16:58:30.62 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:58:30.64 SS.reconfigure SSinhale.with maxSpeed: 23 @16:58:30.73 SS.seek 25ml @16:59:47.25 Stroke completed -- waiting for pressure to equalize ... @16:59:56.04 Intake.select :close @16:59:56.23 SSV.dial :exhaust @16:59:57.12 Exhaust.select :open @16:59:57.26 SS.reconfigure SSexhale @16:59:57.34 SS.seek 0ml @17:00:06.96 Exhaust.select :close @17:00:07.10 SSV.dial CBV @17:00:07.13 CBV.dial SSV @17:00:08.03 Allowing 130s for 25.0ml of remaining 1350.0ml @17:00:08.05 Intake.select :open @17:00:10.26 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:00:10.29 SS.reconfigure SSinhale.with maxSpeed: 23 @17:00:10.38 SS.seek 25ml @17:01:26.82 Stroke completed -- waiting for pressure to equalize ... @17:01:35.62 Intake.select :close @17:01:35.80 SSV.dial :exhaust @17:01:36.68 Exhaust.select :open @17:01:36.83 SS.reconfigure SSexhale @17:01:36.90 SS.seek 0ml @17:01:46.59 Exhaust.select :close @17:01:46.74 SSV.dial CBV @17:01:46.77 CBV.dial SSV @17:01:47.67 Allowing 130s for 25.0ml of remaining 1325.0ml @17:01:47.69 Intake.select :open @17:01:49.90 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:01:49.93 SS.reconfigure SSinhale.with maxSpeed: 23 @17:01:50.03 SS.seek 25ml @17:02:42.83 Vacuum too high -- waiting up to 40s @17:02:46.66 Intake @15.3psia, vacuum from 8.8 to 12.8psi @17:02:46.69 SS.reconfigure SSinhale.with maxSpeed: 21 @17:02:46.78 SS.seek 25ml @17:03:13.73 Stroke completed -- waiting for pressure to equalize ... @17:03:20.63 Intake.select :close @17:03:20.81 SSV.dial :exhaust @17:03:21.68 Exhaust.select :open @17:03:21.82 SS.reconfigure SSexhale @17:03:21.89 SS.seek 0ml @17:03:31.67 Exhaust.select :close @17:03:31.82 SSV.dial CBV @17:03:31.85 CBV.dial SSV @17:03:32.73 Allowing 130s for 25.0ml of remaining 1300.0ml @17:03:32.75 Intake.select :open @17:03:34.97 Intake @15.7psia, vacuum from 9.2 to 13.2psi @17:03:34.99 SS.reconfigure SSinhale.with maxSpeed: 21 @17:03:35.08 SS.seek 25ml @17:04:58.82 Stroke completed -- waiting for pressure to equalize ... @17:05:06.37 Intake.select :close @17:05:06.55 SSV.dial :exhaust @17:05:07.43 Exhaust.select :open @17:05:07.57 SS.reconfigure SSexhale @17:05:07.64 SS.seek 0ml @17:05:17.24 Exhaust.select :close @17:05:17.39 SSV.dial CBV @17:05:17.42 CBV.dial SSV @17:05:18.31 Allowing 130s for 25.0ml of remaining 1275.0ml @17:05:18.33 Intake.select :open @17:05:20.54 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:05:20.57 SS.reconfigure SSinhale.with maxSpeed: 21 @17:05:20.67 SS.seek 25ml @17:06:44.56 Stroke completed -- waiting for pressure to equalize ... @17:06:53.25 Intake.select :close @17:06:53.42 SSV.dial :exhaust @17:06:54.29 Exhaust.select :open @17:06:54.44 SS.reconfigure SSexhale @17:06:54.52 SS.seek 0ml @17:07:04.57 Exhaust.select :close @17:07:04.71 SSV.dial CBV @17:07:04.74 CBV.dial SSV @17:07:05.63 Allowing 130s for 25.0ml of remaining 1250.0ml @17:07:05.65 Intake.select :open @17:07:07.86 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:07:07.88 SS.reconfigure SSinhale.with maxSpeed: 21 @17:07:07.98 SS.seek 25ml @17:08:31.87 Stroke completed -- waiting for pressure to equalize ... @17:08:39.43 Intake.select :close @17:08:39.61 SSV.dial :exhaust @17:08:40.49 Exhaust.select :open @17:08:40.63 SS.reconfigure SSexhale @17:08:40.70 SS.seek 0ml @17:08:50.31 Exhaust.select :close @17:08:50.45 SSV.dial CBV @17:08:50.48 CBV.dial SSV @17:08:51.37 Allowing 130s for 25.0ml of remaining 1225.0ml @17:08:51.39 Intake.select :open @17:08:53.60 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:08:53.63 SS.reconfigure SSinhale.with maxSpeed: 21 @17:08:53.72 SS.seek 25ml @17:10:17.55 Stroke completed -- waiting for pressure to equalize ... @17:10:26.36 Intake.select :close @17:10:27.04 SSV.dial :exhaust @17:10:27.93 Exhaust.select :open @17:10:28.08 SS.reconfigure SSexhale @17:10:28.15 SS.seek 0ml @17:10:37.77 Exhaust.select :close @17:10:37.92 SSV.dial CBV @17:10:37.95 CBV.dial SSV @17:10:38.85 Allowing 130s for 25.0ml of remaining 1200.0ml @17:10:38.87 Intake.select :open @17:10:41.09 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:10:41.11 SS.reconfigure SSinhale.with maxSpeed: 21 @17:10:41.21 SS.seek 25ml @17:12:05.21 Stroke completed -- waiting for pressure to equalize ... @17:12:14.01 Intake.select :close @17:12:14.18 SSV.dial :exhaust @17:12:15.07 Exhaust.select :open @17:12:15.21 SS.reconfigure SSexhale @17:12:15.29 SS.seek 0ml @17:12:24.82 Exhaust.select :close @17:12:24.96 SSV.dial CBV @17:12:24.99 CBV.dial SSV @17:12:25.88 Allowing 130s for 25.0ml of remaining 1175.0ml @17:12:25.90 Intake.select :open @17:12:28.11 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:12:28.14 SS.reconfigure SSinhale.with maxSpeed: 21 @17:12:28.23 SS.seek 25ml @17:13:52.19 Stroke completed -- waiting for pressure to equalize ... @17:14:01.62 Intake.select :close @17:14:01.80 SSV.dial :exhaust @17:14:02.69 Exhaust.select :open @17:14:02.84 SS.reconfigure SSexhale @17:14:02.91 SS.seek 0ml @17:14:12.58 Exhaust.select :close @17:14:12.73 SSV.dial CBV @17:14:12.75 CBV.dial SSV @17:14:13.65 Allowing 130s for 25.0ml of remaining 1150.0ml @17:14:13.67 Intake.select :open @17:14:15.88 Intake @15.5psia, vacuum from 9.0 to 13.0psi @17:14:15.90 SS.reconfigure SSinhale.with maxSpeed: 21 @17:14:16.00 SS.seek 25ml @17:15:39.71 Stroke completed -- waiting for pressure to equalize ... @17:15:48.43 Intake.select :close @17:15:48.61 SSV.dial :exhaust @17:15:49.49 Exhaust.select :open @17:15:49.64 SS.reconfigure SSexhale @17:15:49.71 SS.seek 0ml @17:15:59.46 Exhaust.select :close @17:15:59.61 SSV.dial CBV @17:15:59.64 CBV.dial SSV @17:16:00.52 Allowing 130s for 25.0ml of remaining 1125.0ml @17:16:00.54 Intake.select :open @17:16:02.75 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:16:02.78 SS.reconfigure SSinhale.with maxSpeed: 21 @17:16:02.87 SS.seek 25ml @17:17:26.49 Stroke completed -- waiting for pressure to equalize ... @17:17:35.92 Intake.select :close @17:17:36.10 SSV.dial :exhaust @17:17:36.99 Exhaust.select :open @17:17:37.13 SS.reconfigure SSexhale @17:17:37.20 SS.seek 0ml @17:17:46.40 Exhaust.select :close @17:17:46.55 SSV.dial CBV @17:17:46.57 CBV.dial SSV @17:17:47.47 Allowing 130s for 25.0ml of remaining 1100.0ml @17:17:47.49 Intake.select :open @17:17:49.70 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:17:49.73 SS.reconfigure SSinhale.with maxSpeed: 21 @17:17:49.83 SS.seek 25ml @17:19:13.76 Stroke completed -- waiting for pressure to equalize ... @17:19:23.66 Intake.select :close @17:19:23.85 SSV.dial :exhaust @17:19:24.75 Exhaust.select :open @17:19:24.90 SS.reconfigure SSexhale @17:19:24.97 SS.seek 0ml @17:19:34.63 Exhaust.select :close @17:19:34.78 SSV.dial CBV @17:19:34.81 CBV.dial SSV @17:19:35.71 Allowing 130s for 25.0ml of remaining 1075.0ml @17:19:35.73 Intake.select :open @17:19:37.95 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:19:37.97 SS.reconfigure SSinhale.with maxSpeed: 21 @17:19:38.07 SS.seek 25ml @17:20:22.82 Vacuum too high -- waiting up to 40s @17:20:25.90 Intake @15.3psia, vacuum from 8.8 to 12.8psi @17:20:25.92 SS.reconfigure SSinhale.with maxSpeed: 19 @17:20:26.01 SS.seek 25ml @17:21:09.99 Stroke completed -- waiting for pressure to equalize ... @17:21:18.76 Intake.select :close @17:21:18.94 SSV.dial :exhaust @17:21:19.82 Exhaust.select :open @17:21:19.96 SS.reconfigure SSexhale @17:21:20.03 SS.seek 0ml @17:21:29.27 Exhaust.select :close @17:21:29.41 SSV.dial CBV @17:21:29.44 CBV.dial SSV @17:21:30.33 Allowing 130s for 25.0ml of remaining 1050.0ml @17:21:30.35 Intake.select :open @17:21:32.57 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:21:32.59 SS.reconfigure SSinhale.with maxSpeed: 19 @17:21:32.69 SS.seek 25ml @17:23:05.47 Stroke completed -- waiting for pressure to equalize ... @17:23:16.05 Intake.select :close @17:23:16.24 SSV.dial :exhaust @17:23:17.13 Exhaust.select :open @17:23:17.28 SS.reconfigure SSexhale @17:23:17.35 SS.seek 0ml @17:23:26.58 Exhaust.select :close @17:23:26.72 SSV.dial CBV @17:23:26.75 CBV.dial SSV @17:23:27.66 Allowing 130s for 25.0ml of remaining 1025.0ml @17:23:27.68 Intake.select :open @17:23:29.89 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:23:29.91 SS.reconfigure SSinhale.with maxSpeed: 19 @17:23:30.01 SS.seek 25ml @17:25:02.60 Stroke completed -- waiting for pressure to equalize ... @17:25:13.27 Intake.select :close @17:25:13.45 SSV.dial :exhaust @17:25:14.33 Exhaust.select :open @17:25:14.48 SS.reconfigure SSexhale @17:25:14.55 SS.seek 0ml @17:25:24.13 Exhaust.select :close @17:25:24.28 SSV.dial CBV @17:25:24.31 CBV.dial SSV @17:25:25.19 Allowing 130s for 25.0ml of remaining 1000.0ml @17:25:25.21 Intake.select :open @17:25:27.41 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:25:27.44 SS.reconfigure SSinhale.with maxSpeed: 19 @17:25:27.54 SS.seek 25ml @17:27:00.28 Stroke completed -- waiting for pressure to equalize ... @17:27:09.60 Intake.select :close @17:27:09.78 SSV.dial :exhaust @17:27:10.68 Exhaust.select :open @17:27:10.83 SS.reconfigure SSexhale @17:27:10.90 SS.seek 0ml @17:27:20.50 Exhaust.select :close @17:27:20.65 SSV.dial CBV @17:27:20.68 CBV.dial SSV @17:27:21.58 Allowing 130s for 25.0ml of remaining 975.0ml @17:27:21.60 Intake.select :open @17:27:23.82 Intake @15.5psia, vacuum from 9.0 to 13.0psi @17:27:23.85 SS.reconfigure SSinhale.with maxSpeed: 19 @17:27:23.95 SS.seek 25ml @17:28:56.53 Stroke completed -- waiting for pressure to equalize ... @17:29:05.98 Intake.select :close @17:29:06.16 SSV.dial :exhaust @17:29:07.03 Exhaust.select :open @17:29:07.19 SS.reconfigure SSexhale @17:29:07.26 SS.seek 0ml @17:29:16.89 Exhaust.select :close @17:29:17.04 SSV.dial CBV @17:29:17.07 CBV.dial SSV @17:29:17.96 Allowing 130s for 25.0ml of remaining 950.0ml @17:29:17.98 Intake.select :open @17:29:20.18 Intake @15.5psia, vacuum from 9.0 to 13.0psi @17:29:20.21 SS.reconfigure SSinhale.with maxSpeed: 19 @17:29:20.30 SS.seek 25ml @17:30:52.99 Stroke completed -- waiting for pressure to equalize ... @17:31:04.26 Intake.select :close @17:31:04.44 SSV.dial :exhaust @17:31:05.33 Exhaust.select :open @17:31:05.47 SS.reconfigure SSexhale @17:31:05.54 SS.seek 0ml @17:31:14.78 Exhaust.select :close @17:31:14.92 SSV.dial CBV @17:31:14.95 CBV.dial SSV @17:31:16.35 Allowing 130s for 25.0ml of remaining 925.0ml @17:31:16.38 Intake.select :open @17:31:18.58 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:31:18.60 SS.reconfigure SSinhale.with maxSpeed: 19 @17:31:18.70 SS.seek 25ml @17:32:51.84 Stroke completed -- waiting for pressure to equalize ... @17:33:15.59 Intake.select :close @17:33:15.77 SSV.dial :exhaust @17:33:16.66 Exhaust.select :open @17:33:16.80 SS.reconfigure SSexhale @17:33:16.87 SS.seek 0ml @17:33:26.63 Exhaust.select :close @17:33:26.77 SSV.dial CBV @17:33:26.80 CBV.dial SSV @17:33:27.71 Allowing 130s for 25.0ml of remaining 900.0ml @17:33:27.73 Intake.select :open @17:33:29.94 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:33:29.97 SS.reconfigure SSinhale.with maxSpeed: 19 @17:33:30.06 SS.seek 25ml @17:35:02.63 Stroke completed -- waiting for pressure to equalize ... @17:35:15.02 Intake.select :close @17:35:15.21 SSV.dial :exhaust @17:35:16.08 Exhaust.select :open @17:35:16.23 SS.reconfigure SSexhale @17:35:16.30 SS.seek 0ml @17:35:25.92 Exhaust.select :close @17:35:26.05 SSV.dial CBV @17:35:26.08 CBV.dial SSV @17:35:26.97 Allowing 130s for 25.0ml of remaining 875.0ml @17:35:26.99 Intake.select :open @17:35:29.19 Intake @15.6psia, vacuum from 9.1 to 13.1psi @17:35:29.22 SS.reconfigure SSinhale.with maxSpeed: 19 @17:35:29.31 SS.seek 25ml @17:37:02.20 Stroke completed -- waiting for pressure to equalize ... @17:37:32.06 Intake.select :close @17:37:32.25 SSV.dial :exhaust @17:37:33.15 Exhaust.select :open @17:37:33.30 SS.reconfigure SSexhale @17:37:33.38 SS.seek 0ml @17:37:42.86 Exhaust.select :close @17:37:43.00 SSV.dial CBV @17:37:43.03 CBV.dial SSV @17:37:43.94 Allowing 130s for 25.0ml of remaining 850.0ml @17:37:43.96 Intake.select :open @17:37:46.17 Intake @15.5psia, vacuum from 9.0 to 13.0psi @17:37:46.20 SS.reconfigure SSinhale.with maxSpeed: 19 @17:37:46.28 SS.seek 25ml @17:39:19.44 Stroke completed -- waiting for pressure to equalize ... @17:39:53.97 SS.reconfigure SSconfig @17:39:54.04 SS.hold @17:39:54.19 Intake.select :close @17:39:54.37 ShallowSampler::Clogged in WCR -- progress too slow after 1175.0ml @17:39:54.39 SSV.dial :exhaust @17:39:55.28 Exhaust.select :open @17:39:55.43 SS.reconfigure SSexhale @17:39:55.50 SS.seek 0ml @17:40:05.03 Exhaust.select :close @17:40:05.18 Sampled 1175.0ml @17:40:05.20 Drying puck before fixing for 4:00 @17:40:05.22 CBV.dial :waste2b @17:40:06.13 delay 1:00 @17:41:06.17 CSV.dial :air @17:41:07.14 CS.seek 10ml @17:41:34.06 Pumping wcr puck from tube 2 up to 55psi... @17:41:34.08 CBV.dial SSV @17:41:35.55 CBV.dial between CSV and waste2b @17:41:36.67 CSV.dial between air and CTV @17:41:37.07 CS.seek 7.184ml @17:41:45.35 CTV.dial CSV,avoiding: :air @17:41:45.38 CSV.dial CTV @17:41:46.32 CS.reconfigure CSpressurize @17:41:46.40 CS.seek 0ml @17:42:02.51 CBV.dial :waste2b @17:42:02.90 CS.reconfigure CSconfig @17:42:02.98 CS.seek :empty @17:42:06.99 delay 1:00 @17:43:07.01 CTV.dial :air,avoiding: :intake @17:43:08.57 Fix #1 of 2 (with rnal) for 2:00 @17:43:08.59 CSV.dial CSR @17:43:09.18 Pulling 2ml of CSR.rnal @17:43:09.20 CSR.select :rnal @17:43:09.35 CS.seek 2ml @17:43:15.46 delay 5 seconds @17:43:20.50 CSR.select :air @17:43:20.65 CS.reconfigure CSfast @17:43:20.72 CS.seek 10ml @17:43:42.24 Pumping wcr puck from tube 2 up to 55psi... @17:43:42.26 CBV.dial SSV @17:43:43.71 CBV.dial between CSV and waste2b @17:43:44.83 CSV.dial between air and CTV @17:43:45.55 CS.seek 7.154ml @17:43:54.66 CTV.dial CSV,avoiding: :air @17:43:54.69 CSV.dial CTV @17:43:56.30 CS.reconfigure CSpressurize @17:43:56.38 CS.seek 0ml @17:44:13.98 delay 2:00 @17:46:14.02 CBV.dial :waste2b @17:46:14.41 CS.reconfigure CSconfig @17:46:14.48 CS.seek :empty @17:46:16.96 delay 1:00 @17:47:16.99 CTV.dial :air,avoiding: :intake @17:47:18.56 Fix #2 of 2 (with rnal) for 2:00 @17:47:18.58 CSV.dial CSR @17:47:19.18 Pulling 2ml of CSR.rnal @17:47:19.20 CSR.select :rnal @17:47:19.35 CS.seek 2ml @17:47:25.50 delay 5 seconds @17:47:30.53 CSR.select :air @17:47:30.68 CS.reconfigure CSfast @17:47:30.75 CS.seek 10ml @17:47:52.18 Pumping wcr puck from tube 2 up to 55psi... @17:47:52.20 CBV.dial SSV @17:47:53.65 CBV.dial between CSV and waste2b @17:47:54.79 CSV.dial between air and CTV @17:47:55.51 CS.seek 7.116ml @17:48:03.98 CTV.dial CSV,avoiding: :air @17:48:04.02 CSV.dial CTV @17:48:05.63 CS.reconfigure CSpressurize @17:48:05.71 CS.seek 0ml @17:48:23.00 delay 2:00 @17:50:23.03 CBV.dial :waste2b @17:50:23.41 CS.reconfigure CSconfig @17:50:23.48 CS.seek :empty @17:50:26.15 delay 1:00 @17:51:26.18 CTV.dial :air,avoiding: :intake @17:51:27.80 Storing wcr puck in Collection Clamp to tube 1 @17:51:28.02 SC.reconfigure SCtight @17:51:28.10 SC.seek 1 @17:51:30.28 SC.reconfigure SCloose @17:51:30.53 Elbow.reconfigure TightElbow @17:51:30.61 Elbow.seek 1 @17:51:32.56 Forearm.reconfigure TightForearm @17:51:32.65 Forearm.seek 1 + 700 counts @17:51:33.70 SE.reconfigure SEpick @17:51:33.80 SE.seek beamBreak @17:51:36.77 SE.configure SEconfig @17:51:36.85 SE.seek :place @17:51:37.42 Forearm.reconfigure LooseForearm @17:51:37.50 Forearm.seek :retract @17:51:38.50 Elbow.seek CC @17:51:40.19 Elbow.reconfigure LooseElbow @17:51:40.32 Forearm.seek CC @17:51:41.40 Hand.close @17:51:42.16 CC.seek :open @17:51:46.73 Forearm.seek :retract @17:51:47.77 Elbow.reconfigure TightElbow @17:51:47.85 Elbow.seek 1 @17:51:49.83 Elbow.reconfigure LimpElbow @17:51:49.92 Forearm.seek 1 @17:51:50.96 Elbow.hold @17:51:51.06 Elbow.reconfigure TightElbow @17:51:51.13 Hand.open @17:51:52.03 Forearm.force -20 @17:51:52.09 Forearm.seek 1 + 350 counts @17:51:52.43 Forearm.reconfigure TightForearm @17:51:52.50 Forearm.seek 1 + 700 counts @17:51:52.88 SE.seek :down @17:51:54.04 Forearm.reconfigure LooseForearm @17:51:54.12 Forearm.seek :retract @17:51:55.79 SE.coast @17:51:55.84 SC.coast @17:51:55.91 Starting finalPurge @17:51:55.93 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:51:56.03 Hand.open @17:51:56.40 Elbow.seek FlushPuck::Garage @17:51:58.42 Elbow.reconfigure LooseElbow @17:51:58.55 Forearm.seek :garage @17:51:59.62 Hand.close @17:52:00.51 Forearm.seek :retract @17:52:01.56 Elbow.reconfigure TightElbow @17:52:01.63 Elbow.seek CC @17:52:04.52 Elbow.reconfigure LooseElbow @17:52:04.65 Forearm.reconfigure TightForearm @17:52:04.72 Forearm.seek CC @17:52:06.09 CC.seek :closed @17:52:10.49 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.42 @17:52:10.60 Hand.open @17:52:11.45 Forearm.reconfigure LooseForearm @17:52:11.52 Forearm.seek :retract @17:52:12.59 Flushing the Collection Valve::Manifold @17:52:12.75 CSV.dial CSR @17:52:13.84 Pulling 5ml of CSR.flush @17:52:13.85 CSR.select :flush @17:52:14.02 CS.seek 5ml @17:52:28.06 delay 5 seconds @17:52:33.09 CSR.select :air @17:52:33.29 CS.reconfigure CSfast @17:52:33.36 CS.seek 7.5ml @17:52:40.75 CSV.dial :waste @17:52:42.38 CS.reconfigure CSconfig @17:52:42.45 CS.seek 0ml @17:53:02.67 Flushing lower manifold's waste1 port @17:53:02.83 CSV.dial :flush @17:53:03.38 CS.reconfigure CSfast @17:53:03.45 CS.seek 5ml @17:53:17.34 delay 5 seconds @17:53:22.37 CSV.dial :air @17:53:22.97 CS.seek 7ml @17:53:29.06 CSV.dial CTV @17:53:29.10
CTV.dial CSV @17:53:30.72 CBV.dial :waste1 @17:53:31.64 CS.seek 0ml @17:53:50.63 Flushing lower manifold's waste2b port @17:53:50.80 CSV.dial :flush @17:53:51.67 CS.seek 5ml @17:54:05.47 delay 5 seconds @17:54:10.50 CSV.dial :air @17:54:11.12 CS.seek 7ml @17:54:17.28 CSV.dial CTV @17:54:17.32
CTV.dial CSV @17:54:17.84 CBV.dial :waste2b @17:54:18.74 CS.seek 0ml @17:54:37.56 Flushing upper manifold @17:54:37.72 CSV.dial :flush @17:54:38.59 CS.seek 5ml @17:54:52.41 delay 5 seconds @17:54:57.44 CSV.dial :air @17:54:58.05 CS.seek 7ml @17:55:04.41 CSV.dial CBV @17:55:04.45
CBV.dial CSV @17:55:05.69 CTV.dial :air @17:55:07.24 CS.seek 0ml @17:55:26.06 delay 7 seconds @17:55:33.10 CS.seek 3ml @17:55:41.75 CSV.dial :waste @17:55:42.29 CS.seek 0ml @17:55:51.06 CSV.dial :flush @17:55:51.59 CS.seek 3ml @17:56:00.25 delay 5 seconds @17:56:05.28 CSV.dial :air @17:56:05.87 CS.seek 5ml @17:56:12.06 CSV.dial :air @17:56:12.09 CS.seek 0ml @17:56:25.85 delay 7 seconds @17:56:32.89 CS.seek 3ml @17:56:41.56 CSV.dial :waste @17:56:42.42 CS.seek 0ml @17:56:51.16 Disinfecting external Intake with mfbkill @17:56:51.32 CSV.dial CSR @17:56:53.01 Pulling 8ml of CSR.mfbkill @17:56:53.02 CSR.select :mfbkill @17:56:53.17 CS.seek 8ml @17:57:14.66 delay 5 seconds @17:57:19.69 CSR.select :air @17:57:19.84 CS.seek 10ml @17:57:26.03 CSV.dial CBV @17:57:26.06
CBV.dial CSV @17:57:27.24 CTV.dial :intake @17:57:28.11 Intake.select :open @17:57:28.25 CS.seek 2ml @17:57:50.91 Intake.select :close @17:57:51.04 CTV.dial :air @17:57:52.45 CS.reconfigure CSconfig @17:57:52.52 CS.seek 0ml @17:57:58.60 Flushing lower manifold's waste1 port @17:57:58.62 CSV.dial CSR @17:57:59.90 Pulling 4ml of CSR.mfbkill @17:57:59.91 CSR.select :mfbkill @17:58:00.08 CS.seek 4ml @17:58:11.25 delay 5 seconds @17:58:16.28 CSR.select :air @17:58:16.42 CS.reconfigure CSfast @17:58:16.49 CS.seek 10ml @17:58:32.82 CSV.dial CTV @17:58:32.86
CTV.dial CSV @17:58:34.49 CBV.dial :waste1 @17:58:35.03 CS.seek 0ml @17:59:01.41 Flushing lower manifold's waste2b port @17:59:01.43 CSV.dial CSR @17:59:02.04 Pulling 4ml of CSR.mfbkill @17:59:02.05 CSR.select :mfbkill @17:59:02.21 CS.reconfigure CSconfig @17:59:02.28 CS.seek 4ml @17:59:13.57 delay 5 seconds @17:59:18.60 CSR.select :air @17:59:18.74 CS.reconfigure CSfast @17:59:18.81 CS.seek 10ml @17:59:35.07 CSV.dial CTV @17:59:35.11
CTV.dial CSV @17:59:35.64 CBV.dial :waste2b @17:59:36.54 CS.seek 0ml @18:00:02.78 Killing upper manifold @18:00:02.79 CSV.dial CSR @18:00:03.41 Pulling 4ml of CSR.mfbkill @18:00:03.42 CSR.select :mfbkill @18:00:03.58 CS.reconfigure CSconfig @18:00:03.65 CS.seek 4ml @18:00:14.94 delay 5 seconds @18:00:19.97 CSR.select :air @18:00:20.12 CS.reconfigure CSfast @18:00:20.19 CS.seek 10ml @18:00:36.50 CSV.dial CBV @18:00:36.54
CBV.dial CSV @18:00:37.77 CTV.dial :air @18:00:39.33 CS.seek 0ml @18:01:05.94 delay 7 seconds @18:01:12.98 CS.seek 3ml @18:01:21.69 CSV.dial :waste @18:01:22.22 CS.seek 0ml @18:01:30.91 Flushing upper manifold @18:01:31.07 CSV.dial :flush @18:01:31.59 CS.seek 5ml @18:01:45.44 delay 5 seconds @18:01:50.47 CSV.dial :air @18:01:51.06 CS.seek 7ml @18:01:57.10 CSV.dial CBV @18:01:57.14
CBV.dial CSV @18:01:58.46 CTV.dial :air @18:01:58.49 CS.seek 0ml @18:02:17.38 delay 7 seconds @18:02:24.42 CS.seek 3ml @18:02:33.13 CSV.dial :waste @18:02:33.66 CS.seek 0ml @18:02:42.44 CSV.dial :flush @18:02:42.95 CS.seek 3ml @18:02:51.69 delay 5 seconds @18:02:56.72 CSV.dial :air @18:02:57.43 CS.seek 5.001ml @18:03:03.60 CSV.dial :air @18:03:03.63 CS.seek 0ml @18:03:17.29 delay 7 seconds @18:03:24.33 CS.seek 3ml @18:03:32.91 CSV.dial :waste @18:03:33.77 CS.seek 0ml @18:03:42.44 Flushing lower manifold's waste1 port @18:03:42.60 CSV.dial :flush @18:03:43.14 CS.seek 5ml @18:03:57.51 delay 5 seconds @18:04:02.54 CSV.dial :air @18:04:03.29 CS.seek 7ml @18:04:09.47 CSV.dial CTV @18:04:09.51
CTV.dial CSV @18:04:11.16 CBV.dial :waste1 @18:04:11.72 CS.seek 0ml @18:04:30.41 Flushing lower manifold's waste2b port @18:04:30.57 CSV.dial :flush @18:04:31.44 CS.seek 5ml @18:04:45.22 delay 5 seconds @18:04:50.25 CSV.dial :air @18:04:50.87 CS.seek 7ml @18:04:56.98 CSV.dial CTV @18:04:57.02
CTV.dial CSV @18:04:57.54 CBV.dial :waste2b @18:04:58.44 CS.seek 0ml @18:05:17.16 Disinfecting external Intake with kill @18:05:17.32 CSV.dial CSR @18:05:17.90 Pulling 8ml of CSR.kill @18:05:17.91 CSR.select :kill @18:05:18.07 CS.seek 8ml @18:05:39.85 delay 5 seconds @18:05:44.88 CSR.select :air @18:05:45.03 CS.seek 10ml @18:05:51.07 CSV.dial CBV @18:05:51.11
CBV.dial CSV @18:05:52.33 CTV.dial :intake @18:05:53.23 Intake.select :open @18:05:53.37 CS.seek 2ml @18:06:15.69 Intake.select :close @18:06:15.82 CTV.dial :air @18:06:16.71 CS.reconfigure CSconfig @18:06:16.79 CS.seek 0ml @18:06:22.98 CSV.dial CSR @18:06:24.22 Pulling 5ml of CSR.flush @18:06:24.23 CSR.select :flush @18:06:24.40 CS.seek 5ml @18:06:38.19 delay 5 seconds @18:06:43.21 CSR.select :air @18:06:43.35 CS.seek 10ml @18:06:57.09 CSV.dial CBV @18:06:58.27 CS.seek 0ml @18:07:24.79 Garaging FlushPuck in Collection Clamp @18:07:24.87 Elbow.reconfigure TightElbow @18:07:24.94 Elbow.seek CC @18:07:25.18 Elbow.reconfigure LooseElbow @18:07:25.29 Forearm.seek CC @18:07:26.34 Hand.close @18:07:27.15 CC.seek :open @18:07:31.56 Forearm.seek :retract @18:07:32.63 Elbow.reconfigure TightElbow @18:07:32.69 Elbow.seek :garage @18:07:35.89 Elbow.reconfigure LooseElbow @18:07:36.00 Forearm.reconfigure TightForearm @18:07:36.06 Forearm.seek :garage @18:07:37.23 Hand.open @18:07:38.09 Forearm.reconfigure LooseForearm @18:07:38.15 Forearm.seek :retract @18:07:39.19 Completed [wcrPhase2] begun 16:12:57 @18:07:39.25 Asleep after completing TotalWCRTest assays @18:07:39.26 Mission runtime was 1:55:25.825458 @18:07:39.29 GoodNews.email "{1 => 1, 2 => 0}\nCan@18:02:16, 23.1C, 59% humidity, 14.5psia, 12.006V, 0.651A, 0.651A avg, 7.82W",Subject: "Asleep after completing TotalWCRTest assays" @18:07:46.36 Session Ended