@1533735502.38CEST = =msimfastScheduler =tsimfast =ECan =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @refi<; : @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT:\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #Loading /home/esp/esp2/mission/18tun.rb mission .GoodNews.suppressMail "Starting 18tun w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:38:30, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNegNTCphase\\n/home/esp/esp2/mission/18tun.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:38:30, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:38:34, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNegNTCphase" #\Phase [coreNegNTCphase] #==================> STARTED coreNegNTC (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 15:38:39CEST08-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@15:38:34, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*~% h %&K #tLoading sh1 puck from tube 2 into Collection Clamp #"readyMFB"Readying the MFB C'& #"readyMFB"Homing MFB Syringe .\AFV.select :holdingCoil h & F* M&% h %\  A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'%& h &W ."airStroke"\CSV.dial :waste S)'% ."airStroke"\CS.seek 0ml h % C'/'4 E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +889 C'I% h %4 S)H% h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S)K +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'39 h 9Td +100 C':9 h 9kd +100 C'.9 h 9d +100 C'@9 h 9d +100 C'A9 h 9d +100 C'89 h 9d +100 C'29 h 9d +100 C'D9 h 9d +100 C'E9 h 9 d +100 C'F9 h 9 d +100 C'=9 h 96d +100 C'>9 h 9Ld +100 C')9 h 9bd +100 C'I9 h 9xd +100 C'H9 h 9d +100 C'K9 h 9d +100 C'G9 h 9d +100 C'J9 h 9d +100 C'N9 h 9d +100 C'O9 h 9d +100 C'R9 h 9d +100 C'M9 h 9(d +100 C'S9 h 9>d +100 C'B9 h 9Td +100 C'Q9 h 9id +100 C'L9 h 9d +100 C'P9 h 9d +100 C'V9 h 9d +100 C'W9 h 9d +100 C'T9 h 9d +100 C'Z9 h 9d +100 C'U9 h 9  d +100 C'[9 h 9 d +100 C'Y9 h 9 *d +100 C'\\9 h 9 ?d +100 C'X9 h 9 Td +100 C']9 h 9 j d +100 C'`9 h 9 $d +100 C'_9 h 9 (d +100 C'b9 h 9 ,d +100 C'c9 h 9 0d +100 C'a9 h 9 4d +100 C'f9 h 9 8d +100 C'g9 h 9  fill! #-> ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X h  P%? h  C'@ h  F*A h  F*B h  F*C h  F*D9 h 9 F*E% h %&K S)F% h % C'G& h &W C'H% h %4 P%I& h & P%J% h % M&K% h %\  M&L& h &ӷ A(M& h &  A(N% h %X C'O9 h 9 P%P9 h 9 #t-> ESP::Mode #-> $bacmfb #-> mfb #\Starting mfb #pcrBAC sampling at most 1000ml, wcr at most 1000ml .BACfans.suppressMail "Started mfb in tube 2 at 23:02:50CEST08-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@23:02:02, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1000ml, wcr at most 1000ml" F*Q% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'R& #"readyMFB"Readying the MFB h &W #"readyMFB"Homing MFB Syringe .t\SE.seek :down ."readyMFB"\AFV.select :holdingCoil A(S'X F* +6 *T% h %&K ."readyMFB"\ATV.dial :waste2 F*U % h 'X .t\SE.coast A(5 M&V% h %\  !to:Slide::Error :bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475:@axisName"Collection Clamp: mesg"Collection Clamp not Open: @reply0 #-> CC.open .\CC.seek :open C'W( +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *X' +287 h ( C'Y& h & #t-> ESP.ready! C'Z% P%[% h %4 M&\\% h % S)]% h %\  % M&^& h &ӷ A(_& h &  A(`% h %X C'a& h & P%b& h & C'c h  P%d h  P%e h  P%f h  C'g h  F*h h  F*i h  F*j9 h 9 F*k% h %1i S)l% h % C'm& h & C'n% h %4 P%o& h & P%p% h % M&q% h %\  M&r& h &ӷ A(s& h &  A(t% h %X C'u9 h 9 P%v9 h 9 #t-> mfb #\Starting mfb #pcrBAC sampling at most 1000ml, wcr at most 1000ml .BACfans.suppressMail "Started mfb in tube 2 at 23:03:06CEST08-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@23:02:02, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1000ml, wcr at most 1000ml" F*w% #tLoading sh1 puck from tube 2 into Collection Clamp h %1i C'x& h & M&y% h %\  A(z% h %X A({. h ., A(|& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (}& h &  M&~& h &ӷ M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +143 h ( M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M& ( +6 h ( M&\ & h & A( & h &  .t\Forearm.seek 2 + 300 counts M& '; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b & / +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&% h %; .t\Forearm.seek :retract M&'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #collectSample 1000ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +6 h + S)% h % C'"& h &W E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X'R +5065 h 'R ."readyMFB"\ATV.dial :waste .\ARV.dial between carrier and air1 F*?  +11 h  ."readyMFB"\ASV.dial :waste1 F*@  h +1311 ' #t\Stroke completed -- waiting for pressure to equalize ... +50 C'A% h %4 S)B% h % S)C% h % .tIntake.select :close S)0 +6 )D% h % .t\SSV.dial :exhaust S)E  +75 h  .t\Exhaust.select :open S)@ +7 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'D9 h 9Td +100 C')9 h 9kd +100 C'E9 h 9d +100 C'C9 h 9d +100 C'H9 h 9d +100 C'=9 h 9d +100 C'J9 h 9d +100 C'K9 h 9d +100 C'F9 h 9 d +100 C'29 h 9 d +100 C'@9 h 96d +100 C'N9 h 9Ld +100 C'L9 h 9bd +100 C'P9 h 9xd +100 C'G9 h 9d +100 C'<9 h 9d +100 C'T9 h 9d +100 C'V9 h 9d +100 C'Q9 h 9d +100 C'O9 h 9d +100 C'59 h 9d +100 C'U9 h 9(d +100 C'Z9 h 9>d +100 C'#9 h 9Td +100 C'\\9 h 9id +100 C'M9 h 9d +100 C'I9 h 9d +100 C'Y9 h 9d +100 C']9 h 9d +100 C'_9 h 9d +100 C'`9 h 9d +100 C'b9 h 9  d +100 C'c9 h 9 d +100 C'S9 h 9 *d +100 C'R9 h 9 ?d +100 C'e9 h 9 Td +100 C'a9 h 9 j d +100 C'^9 h 9 $d +100 C'f9 h 9 (d +100 C'i9 h 9 ,d +100 C'g9 h 9 0d +100 C'l9 h 9 4d +100 C'[9 h 9 8d +100 C'n9 h 9  exit !o:Storage::Warning:bt["6/home/esp/esp2/utils/storage.rb:531:in `writeAll'"2/home/esp/esp2/utils/storage.rb:526:in `each'"6/home/esp/esp2/utils/storage.rb:526:in `writeAll'"2/home/esp/esp2/utils/storage.rb:522:in `open'"6/home/esp/esp2/utils/storage.rb:522:in `writeAll'"(/home/esp/esp2/utils/storage.rb:538"3/home/esp/esp2/lib/framework.rb:95:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"./home/esp/esp2/lib/threads.rb:739:in `[]'"1/home/esp/esp2/lib/threads.rb:739:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg" ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& h & .t\CC.configure CCconfig S)#.0X h  P%? h  C'@ h  F*A h  F*B h  F*C h  F*D9 h 9 F*E% h %&K S)F% h % C'G& h &W C'H% h %4 P%I& h & P%J% h % M&K% h %\  M&L& h &ӷ A(M& h &  A(N% h %X C'O9 h 9 P%P9 h 9 #t-> CC.unloadPuck 2 C'Q& h &W #t\Storing sh1 puck in Collection Clamp to tube 2 M&R% h %\  A(S% h %X A(T. h ., A(U& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (V& h &  M&W& h &ӷ M&X% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &Y( +142 h ( M&Z& h & A([& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &\\' +89 h ' A(]& h &  .t\SE.seek 19.71 Pucks below Top Plate A(^' +66 h ' M&_& h & A(`& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &a' +26 h ' .t\Elbow.hold M&b, +6 h , .t\Elbow.reconfigure TightElbow A(c- M&$&.,(\ #b h -+ .t\SE.seek 20.04 Pucks below Top Plate A(d' +27 h ' M&e% h % A(f& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&g'; +20 h '; M&h& h & A(i& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &j' +23 h ' .t\SE.seek 21.04 Pucks below Top Plate A(k' +36 h ' M&l& h & M&m% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +89 h '\  .t\Elbow.seek CC M&o(ӷ +143 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.seek CC M&r' +91 h ' .tHand.close M&s/ +150 h / .t\CC.seek :open C't( +416 h ( A(u& h &  A(v& h &  M&w& h &ӷ M&x% h % .t\Forearm.seek :retract M&y'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &z( +143 h ( M&{& h & A(|& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &}' +92 h ' .t\Elbow.hold M&~, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h % A(& h &  .t\Forearm.force -20 M&5 .t\Forearm.seek 2 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &'; +20 h '; M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(% h % ."downElevator"\SE.seek :down A('X +54 h 'X ."downElevator"\SE.coast A(5 +46 ( % h %X M&\ % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +90 h '\  .t\SC.coast A(6 !to: EOFError:bt[ "1/opt/mbari/lib/ruby/1.8/irb.rb:72:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"end of file reached !o:SignalException :\ signoi:bt["1/home/esp/esp2/lib/threads.rb:721:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg" SIGHUP:@timestampu: Time !; !o:Errno::EIO:bt["7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475:\ errnoi\ : mesg"Input/output error #m\EXIT @1533801791.67CEST @1533801851.82CEST = =ECan =tsimfast =hcore =msimfastScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi|; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 02:33:43CET01-Jan-70 .delayUntil "02:33:43.00" +562299 #-> execute '18tun' .GoodNews.suppressMail "Starting 18tun w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:04:19, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 02:33 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNegNTCphase\\n/home/esp/esp2/mission/18tun.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:06:06, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 02:33" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ C' 2\ P% 0\ S)&\ F*\ 9\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% h % .t\SS.configure SSconfig F*#,,'T(^20( b C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:06:11, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNegNTCphase" #\Phase [coreNegNTCphase] #==================> STARTED coreNegNTC (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 10:06:16CEST09-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:06:11, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*~% h %&K #tLoading sh1 puck from tube 2 into Collection Clamp #"readyMFB"Readying the MFB C'& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil M&% F* h %\  A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A('X h & M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'%& h &W ."airStroke"\CSV.dial :waste S)'% h % ."airStroke"\CS.seek 0ml E ;3\\0\\0 C'/'4 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +889 C'I% h %4 S)H% h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S)K +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'39 h 9Td +100 C':9 h 9kd +100 C'.9 h 9d +100 C'@9 h 9d +100 C'A9 h 9d +100 C'89 h 9d +100 C'29 h 9d +100 C'D9 h 9d +100 C'E9 h 9 d +100 C'F9 h 9 d +100 C'=9 h 96d +100 C'>9 h 9Ld +100 C')9 h 9bd +100 C'I9 h 9xd +100 C'H9 h 9d +100 C'K9 h 9d +100 C'G9 h 9d +100 C'J9 h 9d +100 C'N9 h 9d +100 C'O9 h 9d +100 C'R9 h 9d +100 C'M9 h 9(d +100 C'S9 h 9>d +100 C'B9 h 9Td +100 C'Q9 h 9id +100 C'L9 h 9d +100 C'P9 h 9d +100 C'V9 h 9d +100 C'W9 h 9d +100 C'T9 h 9d +100 C'Z9 h 9d +100 C'U9 h 9  d +100 C'[9 h 9 d +100 C'Y9 h 9 *d +100 C'\\9 h 9 ?d +100 C'X9 h 9 Td +100 C']9 h 9 j d +100 C'`9 h 9 $d +100 C'_9 h 9 (d +100 C'b9 h 9 ,d +100 C'c9 h 9 0d +100 C'a9 h 9 4d +100 C'f9 h 9 8d +100 C'g9 h 9  backtrace #-> $mfb #-> $bacmfb #-> qquit !o:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesgu:NameError::message?undefined local variable or method `qquit' for main:Object: name:\ qquit #-> quit !o:Storage::Warning:bt["6/home/esp/esp2/utils/storage.rb:531:in `writeAll'"2/home/esp/esp2/utils/storage.rb:526:in `each'"6/home/esp/esp2/utils/storage.rb:526:in `writeAll'"2/home/esp/esp2/utils/storage.rb:522:in `open'"6/home/esp/esp2/utils/storage.rb:522:in `writeAll'"(/home/esp/esp2/utils/storage.rb:538"3/home/esp/esp2/lib/framework.rb:95:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"1/home/esp/esp2/lib/framework.rb:99:in `call'"3/home/esp/esp2/lib/framework.rb:99:in `atExit'"./home/esp/esp2/lib/threads.rb:739:in `[]'"1/home/esp/esp2/lib/threads.rb:739:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg" ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& h & .t\CC.configure CCconfig S)#.0X h  P%? h  C'@ h  F*A h  F*B h  F*C h  F*D9 h 9 F*E% h %&K S)F% h % C'G& h &W C'H% h %4 P%I& h & P%J% h % M&K% h %\  M&L& h &ӷ A(M& h &  A(N% h %X C'O9 h 9 P%P9 h 9 #t-> CC.unloadPuck 2 C'Q& h &W #t\Storing sh1 puck in Collection Clamp to tube 2 M&R% h %\  A(S% h %X A(T. h ., A(U& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (V& h &  M&W& h &ӷ M&X% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &Y( +142 h ( M&Z& h & A([& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &\\' +89 h ' A(]& h &  .t\SE.seek 19.71 Pucks below Top Plate A(^' +66 h ' M&_& h & A(`& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 2 M&#Q@2^d^2b &a' +26 h ' .t\Elbow.hold M&b, +6 h , .t\Elbow.reconfigure TightElbow A(c- M&$&.,(\ #b h -+ .t\SE.seek 20.04 Pucks below Top Plate A(d' +27 h ' M&e% h % A(f& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&g'; +20 h '; M&h& h & A(i& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &j' +23 h ' .t\SE.seek 21.04 Pucks below Top Plate A(k' +36 h ' M&l& h & M&m% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &n'\ +89 h '\  .t\Elbow.seek CC M&o(ӷ +143 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &q& h &ӷ .t\Forearm.seek CC M&r' +91 h ' .tHand.close M&s/ +150 h / .t\CC.seek :open C't( +416 h ( A(u& h &  A(v& h &  M&w& h &ӷ M&x% h % .t\Forearm.seek :retract M&y'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &z( +143 h ( M&{& h & A(|& h &  .t\Elbow.reconfigure LimpElbow .\Forearm.seek 2 M&$ ,(#b &}' +92 h ' .t\Elbow.hold M&~, +7 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&% h % A(& h &  .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 2 + 300 counts +2 M&'; +20 h '; M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(% h % ."downElevator"\SE.seek :down A('X +54 h 'X ."downElevator"\SE.coast A(5 +46 ( % h %X M&\ % h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +90 h '\  .t\SC.coast A(6 #t-> execute 'tun18' !o:LoadError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"8No 'tun18' script found on /home/esp/esp2/mission:. #-> execute '18tun' .GoodNews.suppressMail "Starting 18tun w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:16:00, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 ."shutdown"\SS.coast #t\Asleep til +0:00:15 S)5 ."shutdown"\CC.coast #t\Continuing at 02:50 ."shutdown"\CS.coast C'6 E ;3\\0\\0 ."shutdown"\PC.coast .tGoodNews.suppressMail "Then, in tube 2: coreNegNTCphase\\n/home/esp/esp2/mission/18tun.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:16:49, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 02:50" P%6 ."shutdown"\Forearm.coast .\Elbow.coast M&5 + C'5 M&6 +29 ."shutdown"Gate.power :core,:OFF .Gate.power :raw,:OFF #\Shutdown +1470 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +120 S) )  h  C' '  h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ .t\SPE.coast F*\ 9\ C' 2\ F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h %&K .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X& h &2 C'?& ."openPC"\PC.reconfigure PCconfig h &3 ."openPC"\PC.home.seek :open .t\CC.reconfigure CCconfig P%$x2 ^22b %@( .t\CC.home.seek :open C'$x2 ^22b 'A( +223 h ( +3 ( +123 ' ."rdyProcessing"\PS.coast P%5 E ;3\\0\\0 .tGoodNews.suppressMail "At 02:50:53 began in tube 2: coreNegNTCphase\\n/home/esp/esp2/mission/18tun.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:16:54, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNegNTCphase" #\Phase [coreNegNTCphase] #==================> STARTED coreNegNTC (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'B& h & .tHand.open M&C1 +150 h 1 M&D& h &_ M&E% h %\  .t\Elbow.seek FlushPuck::Garage M&F(- +165 h (- M&G& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &H& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &I'η +91 h 'η .tHand.close M&J/ +150 h / M&K& h &- M&L% h %η .t\Forearm.seek :retract M&M'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +265 h (ӷ M&O& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Q' +91 h ' .t\CC.seek :closed C'R(W +416 h (W C'S& h &W .tHand.open M&T1 +150 h 1 M&U% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'W& h &W C'X% h %4 .t\CSV.dial CSR C'Z  +150 h  C'[% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ']'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '_% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '`'[ +463 h '[ .t\CSV.dial :waste C'/ +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'c'4 +2010 h '4 #t\Purging external Intake C'e& h &W C'f% h %4 .t\CSV.dial :flush C'h  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'i' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'l 'm  h +76 .t\CTV.dial :intake C'8  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'#'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'\\  +76 h #t\Flushing upper manifold C'Y& h &W C'^% h %4 .t\CSV.dial :flush C'a  +75 h  .t\CS.seek 5ml C'p'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'q @ +38 h @ C'g% h %p .t\CS.seek 7ml C'd'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'j +113 h .t\CTV.dial :air .\CS.seek 0ml C'b'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C's'0 +506 h '0 .t\CSV.dial :waste C'n +38 h .t\CS.seek 0ml C'5'4 +507 h '4 C'u& h &W C'v% h %4 .t\CSV.dial :flush C'w  +37 h  .t\CS.seek 3ml C'x'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'{ @ +37 h @ C'|% h %0 .t\CS.seek 5ml C'r'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'o'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C' +76 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &ӷ .t\Forearm.seek CC M&z' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'\ ( +416 h ( M&}& h &ӷ M&% h % .t\Forearm.seek :retract M&k'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &;& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M& 1 +150 h 1 M&~% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &$'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 10:16:59CEST09-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:16:54, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*t% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K #"readyMFB"Readying the MFB C'!& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil M& % F* h %\  A( % h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F*% h %&K ."readyMFB"\ATV.dial :waste2 F* % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *' +31 h ( M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A("& h &  M&& h & .t\Elbow.seek 2 M&(( +6 h ( M&*& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &+/ +150 h / ."downElevator"\SE.seek :down M&-& A(.'X h & M&'% h %; .t\Forearm.seek :retract M&&'\ +57 h 'X ."downElevator"\SE.coast A(5 +34 h '\  .t\Elbow.seek CC M&(ӷ +143 h (ӷ M&3& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &=' +91 h ' .t\CC.seek :closed C'>(W +416 h (W .tHand.open M&?1 +150 h 1 M&%% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &)'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'7& h &W ."airStroke"\CSV.dial :waste S):% ."airStroke"\CS.seek 0ml h % E ;3\\0\\0 C'B'4 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0Xd +100 C'F9 h 9Td +100 C'M9 h 9kd +100 C'A9 h 9d +100 C'S9 h 9d +100 C'T9 h 9d +100 C'K9 h 9d +100 C'E9 h 9d +100 C'W9 h 9d +100 C'X9 h 9 d +100 C'Z9 h 9 d +100 C'P9 h 96d +100 C'Q9 h 9Ld +100 C'<9 h 9bd +100 C'_9 h 9xd +100 C']9 h 9d +100 C'/9 h 9d +100 C'[9 h 9d +100 C'`9 h 9d +100 C'f9 h 9d +100 C'h9 h 9d +100 C'm9 h 9d +100 C'e9 h 9(d +100 C'89 h 9>d +100 C'U9 h 9Td +100 C'l9 h 9id +100 C'c9 h 9d +100 C'i9 h 9d +100 C'Y9 h 9d +100 C'^9 h 9d +100 C'#9 h 9d +100 C'q9 h 9d +100 C'\\9 h 9  d +100 C'g9 h 9 d +100 C'p9 h 9 *d +100 C'd9 h 9 ?d +100 C'a9 h 9 Td +100 C'j9 h 9 j d +100 C'n9 h 9 $d +100 C's9 h 9 (d +100 C'u9 h 9 ,d +100 C'v9 h 9 0d +100 C'59 h 9 4d +100 C'{9 h 9 8d +100 C'|9 h 9   +18 h  .t\CS.seek 5ml C'/'p +1373 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'[' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'`'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%f  +38 h  .t\PS.seek 2.5ml P%e' +2609 h ' .t\PSV.dial :mixloop P%8 +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%<  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%Z +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%c  +151 h  .t\PS.seek :empty P%i' +2609 h ' .t\CBV.dial :waste1 C'Y @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'#  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'q @ +37 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'g  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'h ` +19 h ` .t\CSV.dial :air C'm @ +38 h @ .t\CS.seek 10ml C'd' +2647 h ' .t\CSV.dial CTV C'a  +37 h  .t\CS.seek 0ml C'U'4 +2648 h '4 .t\CBV.dial :waste1 C'^ @ +18 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'n  +151 h C'X& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&u% h %\  A(v% h %X A(5. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b ({( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (|& h & M&x& h &ӷ M&\\% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &b( +143 h ( M&& h & A(p& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &K' +89 h ' A(O& h & .t\SE.seek -0.29 Pucks below Top Plate A(l'\\ +265 h '\\ M&& h & A(w& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &y'Y +25 h 'Y .t\Elbow.hold M&j, +6 h , .t\Elbow.reconfigure TightElbow A(- h -+\\ M&$&.,(\ #b .t\SE.seek 0.04 Pucks below Top Plate A(' +27 h ' M&V% h %Y A(r& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 & 'υ +20 h 'υ M&]& h & A(& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &R' +23 h ' .t\SE.seek 1.04 Pucks below Top Plate A(' +36 h ' M&;& h & M&z% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +89 h '\  .t\Elbow.seek CC M&k(ӷ +143 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &~& h &ӷ .t\Forearm.seek CC M&t' +91 h ' .tHand.close M&!/ +150 h / .t\CC.seek :open C' ( +416 h ( A(& h & A(& h & M& & h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&& h & A(& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .t\Elbow.hold M&, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&"% h %Y A( & h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&$'υ +20 h 'υ M&-& h & A(N& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &o' +23 h ' A(% h % ."downElevator"\SE.seek :down A('X +100 (% h %S M&+% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &2'\ +89 h '\  +61 'X ."downElevator"\SE.coast .t\SC.coast A(5 .t\PSV.dial :air P%3  + A(6 +112 h  P%% h % .t\PS.seek 0.499ml P%.' +584 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%( +38 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%\  +37 h .t\CBV.dial AIV C'?  +38 h .t\PRV.dial between CBV and air P%&  +19 h  .t\PRV.dial :air P%*  +19 h  P%%% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %D' +250 h ' .t\PRV.dial :waste2 P%= @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'1& h & .tHand.open M&:1 +2 h 1 M&& h & M&4% h %\  .t\Elbow.seek FlushPuck::Garage M&H(- +166 h (- M&9& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &- .t\Forearm.seek :garage M&L'η +91 h 'η .tHand.close M&/ +150 h / M&I& h &- M&% h %η .t\Forearm.seek :retract M&M'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &6(ӷ +264 h (ӷ M&G& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &T& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &E' +91 h ' .t\CC.seek :closed C'J(W +416 h (W C'B& h &W .tHand.open M&F1 +150 h 1 M&,% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &A'\ +91 h '\  C''& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%Q @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%_  +76 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%)  +75 h  .tdelay 2 seconds +200 P%>% h % .t\PS.seek 1.499ml P%W' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%7' +1094 h ' .t\PSV.dial :air P%s  +38 h  .t\PS.seek 0ml P%/' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%[ +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%f  +38 h  .tdelay 2 seconds +200 P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %8'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%<  +75 h .tdelay 2 seconds +200 P%Z% h %r .t\PS.seek 0.475ml P%c' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%e  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%Y'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%#  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%q  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %g' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%h +75 h .t\PTV.dial :PRV,avoiding: :puck P%m @ +76 h @ .t\PS.seek 0ml P%`' +2609 h ' .t\PSV.dial :air P%a  +76 h  .t\PS.seek 2.5ml P%d' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%^ +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%n' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%X  +76 h  .t\PS.seek 2.5ml P%u' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%v +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%5  +75 h P%|% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %x' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'\\ & +50 h & .t\AWV.select :waste C' +7 P%% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %p'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'O { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %S' +1313 h ' .t\PSV.dial :air P%  +37 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :PML P%w +38 h .t\PS.seek 0ml P%' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 'V& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'r& F*] @@T ."MFBPCR"\ASV.dial :SPEdelivery h &W .t\AWV.select :air F*  .t\AIV.dial :bypass .\PSV.dial PSR P%; +22 h  ."MFBPCR"delay 20 seconds +91 h P%z% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%K' +1597 h ' .tdelay 5 seconds +306 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*b I +128 h I F*l% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *y'6 +66 .t\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%~' +1597 h ' .t\PSV.dial PSR P% +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%R +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%j' +1587 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*!  +22 h ' .t\PSV.dial PSR P%} +21 h  F*P% h %6 ."MFBPCR"\AS.seek 0.329ml F*'ͯ +92 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P% ' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F* % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1049 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%t' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*k  +22 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *"' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%-' +217 ."MFBPCR"\ATV.dial :AIV F*N I +43 h I F*% h % ."MFBPCR"\AS.seek 0.469ml F*' +2349 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2610 h ' .t\PSV.dial PSR P%+ +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%$' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +37 h  .t\PS.seek 0ml P%3' +740 h ' ."MFBPCR"delay 5 seconds +500 F*9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*o n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*{'% +336 h ' .t\PSV.dial PSR P%i +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%(' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%\ ' +2610 h ' .t\PSV.dial PSR P%? +113 h P%&% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%*' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%%  +38 h  .t\PS.seek 0ml P%.' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%= +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%D' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%: +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%4  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %9' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F*C I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *'V +850 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%I' +2609 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %2' +5107 h ' .t\PSV.dial :air P%L  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %G' +1326 h 'V ."MFBPCR"\ATV.dial :air2 F*T  +43 h  F*H% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *M'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F* I +43 h I F*B% h %Q ."MFBPCR"\AS.seek 0.01ml F*F' +171 h ' .t\PSV.dial :mixloop P%, +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %'' +618 h ' ."MFBPCR"\ATV.dial :waste F*Q n +129 h n ."MFBPCR"\AS.seek :empty F*E'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*_  +85 h  F*)% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *>' +1023 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*W% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *s'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'7  +38 h  C'[% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'f'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'/  '<  +37 h  +113  .t\CBV.dial :waste1 C'Z @ +38 h @ .t\CS.seek 0ml C'8'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*e  +21 h ."MFBPCR"delay 3 seconds +300 F*c% h %_ ."MFBPCR"\AS.seek 0.434ml F*#'ݏ +110 h '4 .t\CSV.dial CSR C'q  +38 h  C'Y% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'h'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*m  +21 h F*g% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*a'& +324 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*` n +108 h n F*^% h %& ."MFBPCR"\AS.seek 0.092ml F*d'&0 +191 .t\CSR.select :air C' +6 .t\CS.seek 10ml C'X' +1626 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'n  +37 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'v'4 +2648 h '4 C'5& h &W #tGaraging FlushPuck in Collection Clamp M&|& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &u(ӷ +6 h (ӷ M&\\& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&x' +91 h ' .tHand.close M&O/ +150 h / .t\CC.seek :open C'p( +416 h ( M&& h &ӷ M&S% h % .t\Forearm.seek :retract M&w'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +264 h (- M&V& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &r& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&;1 +150 h 1 M&z% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'b& h & M&l% h %\  A( % h %X A(0. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (K( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b ( & h &  M&~& h &- M&R% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +165 h ( M&!& h & A(P& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &}' +90 h ' A(& h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(y' +77 h ' A(j& h &  M&& h & ."WCR"\Elbow.seek 2 M&( +6 h ( M&& h & A(k& h &  ."WCR"\Forearm.seek 2 + 300 counts M&'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b & / +150 h / ."downElevator"\SE.seek :down M&& A( 'X h & M&N% h %; ."WCR"\Forearm.seek :retract M&'\ +28 h '&0 ."MFBPCR"delay 3 seconds +49 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&t(ӷ +143 h (ӷ M&"& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &-' +91 h ' ."WCR"\CC.seek :closed C'+(W +3 ."MFBPCR"\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&1 +150 h 1 M&$% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*o  +21 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +30 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)+ +6 h + S)i% h % C'3& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +8250 h ' #"WCR"\Stroke completed -- waiting for pressure to equalize ... +50 C'7% h %4 S)[% h % S)U% h % ."WCR"Intake.select :close S)0 +6 )f% h % ."WCR"\SSV.dial :exhaust S)/  +2 h ' ."MFBPCR"delay 5 seconds +73 h  ."WCR"\Exhaust.select :open S)@ +7 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X % +22 h % ."MFBPCR"delay 3 seconds +300 F*f% h % ."MFBPCR"\AS.seek 0.255ml F*'X +103 h ' #"WCR"\Stroke completed -- waiting for pressure to equalize ... +50 C'c% h %4 S)q% h % S)% h % ."WCR"Intake.select :close S)0 +6 )M% h % ."WCR"\SSV.dial :exhaust S)e  +76 h  ."WCR"\Exhaust.select :open S)@ +6 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X% h % ."MFBPCR"\AS.seek 0.53ml F*f' +620 h ' ."MFBPCR"delay 10 seconds +354 h ' #"WCR"\Stroke completed -- waiting for pressure to equalize ... +50 C'c% h %4 S)q% h % S)% h % ."WCR"Intake.select :close S)0 +6 )M% h % ."WCR"\SSV.dial :exhaust S)e  +75 h  ."WCR"\Exhaust.select :open S)@ +7 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X  +75 h  #"WCR"\Allowing 130s for 25.0ml of remaining 550.0ml .Intake.select :open S) +206 C',% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X  +75 h  #"WCR"\Allowing 130s for 25.0ml of remaining 225.0ml .Intake.select :open S) +206 C',% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X% h % ."WCR"\CBV.dial between CSV and waste2b C',  +94 h  C'% h % ."WCR"\CSV.dial between air and CTV C'q ` +56 h ` ."WCR"\CS.seek 7.288ml C'' +482 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'M  'e  +19 h  +131  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ' '4 +1919 h '4 ."WCR"delay 2:00 +12000 .\CBV.dial :waste2b C'U  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b '<' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'`  +151 h C'c& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&% h %\  A(/% h %X A(Z. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (^( +200 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (g& h & M&_& h &ӷ M&h% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &\\( +143 h ( M&f& h & A(O& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &6' +89 h ' A(5& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A(8'K +253 h 'K M&Y& h & A(#& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &V'Y +26 h 'Y ."WCR"\Elbow.hold M&J, +6 h , ."WCR"\Elbow.reconfigure TightElbow A(m- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(a' +27 h ' M&X% h %Y A(s& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&n'υ +20 h 'υ M&r& h & A(A& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +23 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(b' +36 h ' M&;& h & M&~% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +89 h '\  ."WCR"\Elbow.seek CC M&l(ӷ +143 h (ӷ M&u& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ ."WCR"\Forearm.seek CC M&z' +91 h ' ."WCR"Hand.close M&!/ +150 h / ."WCR"\CC.seek :open C' ( +416 h ( A(|& h & A(& h & M&x& h &ӷ M&% h % ."WCR"\Forearm.seek :retract M&S'\ +90 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +143 h ( M&0& h & A(& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +92 h 'Y ."WCR"\Elbow.hold M&, +7 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &k1 +150 h 1 M&% h %Y A(j& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&R'υ +20 h 'υ M&& h & A(N& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(% h % ."downElevator"\SE.seek :down A("'X +100 ( % h %B M&$% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  +51 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&o1 +2 A(6 h 1 M&y& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&3(- +166 h (- M&d& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&i/ +150 h / M&\ & h &- M&}% h %η .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &p(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &v& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &:' +90 h ' .t\CC.seek :closed C'.(W +416 h (W C'& h &W .tHand.open M&=1 +150 h 1 M&C% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &-'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'% h % .t\CSV.dial CSR C'(  +38 h  C'*% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'?'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'T% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '1'[ +463 h '[ .t\CSV.dial :waste C'& +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '9'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'4& h &W C'B% h %4 .t\CSV.dial :flush C'%  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'D'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'2 @ +38 h @ C'% h %p .t\CS.seek 7ml C'{'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'G  +37 h  +113  .t\CBV.dial :waste1 C'H @ +75 h @ .t\CS.seek 0ml C'W'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'F& h &W C'7% h %4 .t\CSV.dial :flush C'L  +76 h  .t\CS.seek 5ml C'E'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'I @ +38 h @ C')% h %p .t\CS.seek 7ml C'Q'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'>  +37 h  .t\CBV.dial :waste2b C',  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'q  +38 h  C'[% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'e'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'M' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 C'U @ +75 h @ .t\CS.seek 0ml C' '4 +910 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'`  +38 h  C'c% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '/' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'Z  +38 h  .t\CBV.dial :waste2b C'g  +75 h  .t\CS.seek 0ml C'_'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'h  +37 h  C'f% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'O'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '5' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C't '  +37 h +76 .t\CTV.dial :air C'Y  +150 h .t\CS.seek 0ml C'#'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'm'0 +506 h '0 .t\CSV.dial :waste C'X +38 h .t\CS.seek 0ml C's'4 +507 h '4 #t\Flushing upper manifold C'r& h &W C'A% h %4 .t\CSV.dial :flush C';  +37 h  .t\CS.seek 5ml C'~'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'6 @ +37 h @ C'\\% h %p .t\CS.seek 7ml C'8'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'V +113 h .t\CTV.dial :air .\CS.seek 0ml C'u'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'P'0 +507 h '0 .t\CSV.dial :waste C'n +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'& h &W C'J% h %4 .t\CSV.dial :flush C'!  +37 h  .t\CS.seek 3ml C' '0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'+ @ +38 h @ C'|% h %0 .t\CS.seek 5ml C''p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'x'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +506 h '0 .t\CSV.dial :waste C'0 +76 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing lower manifold's waste1 port C'z& h &W C'l% h %4 .t\CSV.dial :flush C'k  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'j @ +38 h @ C'% h %p .t\CS.seek 7ml C'N'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'S  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C' '4 +766 h '4 #t\Flushing lower manifold's waste2b port C'$& h &W C'% h %4 .t\CSV.dial :flush C'o  +75 h  .t\CS.seek 5ml C'y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C'R'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'b  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'd'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &"(ӷ +7 h (ӷ M&^& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&i' +91 h ' .tHand.close M&\ / +150 h / .t\CC.seek :open C'}( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &v(- +265 h (- M&3& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&1 +150 h 1 M&=% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&(& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &*(- +6 h (- M&:& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &<& h &- .t\Forearm.seek :garage M&T'η +91 h 'η .tHand.close M&?/ +150 h / M&&& h &- M&1% h %η .t\Forearm.seek :retract M&4'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &B(ӷ +265 h (ӷ M&%& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &9& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &2' +90 h ' .t\CC.seek :closed C'(W +416 h (W C'D& h &W .tHand.open M&1 +150 h 1 M&G% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &H'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'{& h &W C'F% h %4 .t\CSV.dial CSR C'7  +38 h  C'L% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'W'p +1373 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ')'[ +463 h '[ .t\CSV.dial :waste C'E +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '>'4 +2010 h '4 #t\Purging external Intake C',& h &W C'Q% h %4 .t\CSV.dial :flush C'q  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '[' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 'e  +38 h +38 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'U'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'M  +76 h #t\Flushing upper manifold C'`& h &W C'c% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +38 h @ C'g% h %p .t\CS.seek 7ml C'/'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'h +113 h .t\CTV.dial :air .\CS.seek 0ml C'f'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +506 h '0 .t\CSV.dial :waste C'O +38 h .t\CS.seek 0ml C''4 +506 h '4 C't& h &W C'Y% h %4 .t\CSV.dial :flush C'5  +38 h  .t\CS.seek 3ml C'#'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'X @ +37 h @ C'm% h %0 .t\CS.seek 5ml C'r'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'A'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C';'0 +507 h '0 .t\CSV.dial :waste C's +75 h .t\CS.seek 0ml C'6'4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&\\& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &~(ӷ +6 h (ӷ M&V& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &8& h &ӷ .t\Forearm.seek CC M&u' +91 h ' .tHand.close M&n/ +150 h / .t\CC.seek :open C'P( +416 h ( M&& h &ӷ M&J% h % .t\Forearm.seek :retract M&!'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &a(- +265 h (- M&+& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &|& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b & 'η +91 h 'η .tHand.open M&1 +150 h 1 M&x% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +91 h '\  #t\Completed [coreNegNTCphase] begun 02:50:53 #\Extending 18tun until completion of MFBPCR +257347 !"PCR"o:#AnalyticalModule::PCRRawSample: @refi; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0104h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*l  +22 h ."MFBPCR"\ATV.dial :air3 F*k % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *j'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*N'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*$  +21 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*o'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *y'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*b' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*\  +31 h  ."MFBPCR"\ATV.dial :air3 F*p % +87 h % F*-% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *.' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*i'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*3 % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*"'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*=% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*(' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *:'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *T'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0106h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*%  +43 h  ."MFBPCR"\ARV.dial :carrier F*9  +11 h  F**% h %V ."MFBPCR"\AS.seek 0.5ml F*4'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*D n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*{  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*F  +22 h ."MFBPCR"delay 2 seconds +200 F*7% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *L'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*2 I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*d% h %Fj ."MFBPCR"\AS.seek 0.524ml F*I' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*W % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*E% h % ."MFBPCR"\AS.seek 0.53ml F*)' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*,  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*q'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*>  +22 h  ."MFBPCR"delay 7 seconds +700 F*e% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*[% h %\  ."MFBPCR"\AS.seek 0.6ml F*M'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*`  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*c% h %\  ."MFBPCR"\AS.seek 0.65ml F* ' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*U  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*Z  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*g% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*h  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*/ % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0106h.pcr F*f% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*_  +22 h ."MFBPCR"\ATV.dial :air3 F*t % +86 h % F*Y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *5'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*X% h %Z ."MFBPCR"\AS.seek 0.81ml F*m'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*# % +22 h % ."MFBPCR"delay 3 seconds +300 F*r% h %> ."MFBPCR"\AS.seek 0.835ml F*A'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*s  +22 h F*;% h %n ."MFBPCR"\AS.seek 0.82ml F*\\'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*V  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*8  +64 h  F*n% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *B'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*H' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*|  +32 h  ."MFBPCR"\ATV.dial :air3 F*~ % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *x' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*!  +21 h F* % h % ."MFBPCR"\AS.seek 0.81ml F*]'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*w% h %> ."MFBPCR"\AS.seek 0.835ml F*l'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*j' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0109h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*o  +43 h  ."MFBPCR"\ARV.dial :carrier F*y  +11 h  F*b% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *^'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\ '(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*p  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*-  +21 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*.% h %Fj ."MFBPCR"\AS.seek 0.524ml F*3' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*i% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*=  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*"% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*('\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*: n +21 h n ."MFBPCR"delay 2 seconds +200 F*<% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*?  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*&% h %\  ."MFBPCR"\AS.seek 0.65ml F*1' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*v  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*C  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*%% h % \\ ."MFBPCR"\AS.seek 0.326ml F*9'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F**  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*T % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0109h.pcr F*4% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*}  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*{% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *F'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*7  +22 h F*G% h %Z ."MFBPCR"\AS.seek 0.81ml F*2'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*d % +21 h % ."MFBPCR"delay 3 seconds +300 F*L% h %> ."MFBPCR"\AS.seek 0.835ml F*W'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*E  +21 h F*I% h %n ."MFBPCR"\AS.seek 0.82ml F*,'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*)  +65 h  F*>% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *e'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*q' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*M  +31 h  ."MFBPCR"\ATV.dial :air3 F*U % +87 h % F*Z% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *g' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*h% h % ."MFBPCR"\AS.seek 0.81ml F*/'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*f% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*_  +21 h F*t% h %m ."MFBPCR"\AS.seek 0.82ml F*Y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*O  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*X' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *8'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0111h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*n  +42 h  ."MFBPCR"\ARV.dial :carrier F*\\  +11 h  F*B% h %V ."MFBPCR"\AS.seek 0.5ml F*H'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *J'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *u'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*|  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*~  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *+'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*! I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*x' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*w% h % ."MFBPCR"\AS.seek 0.53ml F*]' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*k  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*l'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*z% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*j n +21 h n ."MFBPCR"delay 2 seconds +200 F*S% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*$% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*N  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*o'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*y  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*b % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0111h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*R % +86 h % F*^% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *p'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*-  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*\ '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*.% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*i% h %n ."MFBPCR"\AS.seek 0.82ml F*3'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*=  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*"  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*&  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*v% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *C' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +21 h F*1% h % ."MFBPCR"\AS.seek 0.81ml F**'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*4% h %> ."MFBPCR"\AS.seek 0.835ml F*9'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*}  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*{'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*D  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *7'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *E'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *)'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0113h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ARV.dial :carrier F*,  +11 h  F*e% h %V ."MFBPCR"\AS.seek 0.5ml F*q'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*[ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *`'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*M  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*U  +21 h ."MFBPCR"delay 2 seconds +200 F*Z% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *c'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*h% h %Fj ."MFBPCR"\AS.seek 0.524ml F*g' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*f% h % ."MFBPCR"\AS.seek 0.53ml F*/' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*_ % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*t% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*O  +107 h  ."MFBPCR"delay 7 seconds +700 F*Y% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*X n +21 h n ."MFBPCR"delay 2 seconds +200 F*5% h %\  ."MFBPCR"\AS.seek 0.6ml F*#'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*r  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*m% h %\  ."MFBPCR"\AS.seek 0.65ml F*s' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*;  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*A  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*V% h % \\ ."MFBPCR"\AS.seek 0.326ml F*n'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*\\  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*B % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0113h.pcr F*8% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *H'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *|'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*u'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*! % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*+'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*w% h %n ."MFBPCR"\AS.seek 0.82ml F*x'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*k  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*]% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*z' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*$  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*N% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*y'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*b % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*o'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*R% h %m ."MFBPCR"\AS.seek 0.82ml F*^'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *-'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *"'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt270jan0116h.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*3  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*: n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *<'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *('(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*&  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*v% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *?'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*% n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*1% h %Fj ."MFBPCR"\AS.seek 0.524ml F*C' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*T  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*4% h % ."MFBPCR"\AS.seek 0.53ml F**' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*}  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*9'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*D  +21 h  ."MFBPCR"delay 7 seconds +700 F*{% h %< ."MFBPCR"\AS.seek 0.594ml F*7'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*G n +21 h n ."MFBPCR"delay 2 seconds +200 F*F% h %\  ."MFBPCR"\AS.seek 0.6ml F*d'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*L  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*2% h %\  ."MFBPCR"\AS.seek 0.65ml F*E' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*I  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*W  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*Q% h % \\ ."MFBPCR"\AS.seek 0.326ml F*>'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*,  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*e % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt270jan0116h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F*) @@T *q  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*  +150 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *M' +424 h ' ."MFBPCR"\ATV.dial :ASV F*U n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*Z  +43 h  F*`% h % ."MFBPCR"\AS.seek 0.62ml F* '\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*h n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *c'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*f'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*g % +43 h % F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *t' +423 h ' ."MFBPCR"\ATV.dial :air3 F*/% h % ."MFBPCR"\AS.seek 0.53ml F*O' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*% h % ."MFBPCR"\AS.seek 0.71ml F*X' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*5 % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*r' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*m  +22 h F*#% h % Ű ."MFBPCR"\AS.seek 0.72ml F*;' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*A n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*V% h % zG ."MFBPCR"\AS.seek 0.365ml F*s' +7161 h ' ."MFBPCR"\ATV.dial :waste F*\\ n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *B'R +1873 h 'R ."MFBPCR"\ATV.dial :air3 F*8 % +43 h % F*n% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*J% h % ."MFBPCR"\AS.seek 0.579ml F*H' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*~ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*|'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*!  +22 h F* % h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*u' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*w  +21 h  ."MFBPCR"\AS.seek 0ml F*+'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*] % +108 h % F*x% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*z  +22 h F*l% h % ."MFBPCR"\AS.seek 0.58ml F*j' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*$'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*N% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*  +21 h  ."MFBPCR"\AS.seek 0ml F* 'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*y  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*R n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *o'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F* n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *^'R +202 h 'R ."MFBPCR"\SPE.coast F*-  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*p n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*  +43 h  F*.% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *\ ' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *i'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*=  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *3': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*  +65 h  F*"% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*: n +65 h n F*% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *<' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*& I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*(  +43 h  F*%% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *1': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*?  +65 h  F*T% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *4'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*C n +64 h n F*}% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F** I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *D'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*{  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *9': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*G n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *F'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*7 n +151 h n ."MFBPCR"\AS.seek 0ml F*L'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*2  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*d % +42 h % F*I% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *W' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Q  +21 h F*E% h % ."MFBPCR"\AS.seek 0.96ml F*,'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*e % +22 h % ."MFBPCR"delay 3 seconds +300 F*q% h %0 ."MFBPCR"\AS.seek 0.985ml F*>' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*['c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*U I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +199 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *`'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *h'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *c'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*g% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *_' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *f' +9988 h ' ."MFBPCR"\ATV.dial AIV F*/ I +43 h I ."MFBPCR"\AS.seek 0.02ml F*t' +9880 h ' ."MFBPCR"\ATV.dial :waste F*Y n +129 h n ."MFBPCR"\AS.seek 0ml F*'V +226 h 'V ."MFBPCR"\AWV.select :air C' +7 F*O% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *5'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*X % +43 h % F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *#'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*r  +22 h F*A% h %Z ."MFBPCR"\AS.seek 0.81ml F*V'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*; % +22 h % ."MFBPCR"delay 3 seconds +300 F*\\% h %> ."MFBPCR"\AS.seek 0.835ml F*s'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +22 h F*n% h %n ."MFBPCR"\AS.seek 0.82ml F*B'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*J  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *~'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *H'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *|' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*w  +32 h  ."MFBPCR"\ATV.dial :air3 F*u % +86 h % F*+% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *k' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +22 h F*x% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*z % +22 h % ."MFBPCR"delay 3 seconds +300 F*l% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*S  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*j'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *N'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*$' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*- n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *o'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*^ % +108 h % ."MFBPCR"\AS.seek 0ml F*p'V +2547 h 'V #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0119h.pcr #Building PCR Slug from elutionWater with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*  +64 h  ."MFBPCR"\ARV.dial :carrier F*.  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*i n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *='R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *3'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*:  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*&  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*<% h % ."MFBPCR"\AS.seek 0.53ml F* ' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*( % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*%% h % ."MFBPCR"\AS.seek 0.536ml F*v'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*?  +107 h  ."MFBPCR"delay 7 seconds +700 F*T% h %< ."MFBPCR"\AS.seek 0.594ml F*1'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*C I +108 h I ."MFBPCR"delay 2 seconds +200 F*}% h %\  ."MFBPCR"\AS.seek 0.6ml F*4'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F**  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*{' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*D  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*G  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*9% h % \\ ."MFBPCR"\AS.seek 0.326ml F*7'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*F  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*2 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0119h.pcr F*d% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *I'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*L  +22 h ."MFBPCR"\ATV.dial :air3 F*Q % +86 h % F*E% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *W'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*e  +22 h F*q% h %Z ."MFBPCR"\AS.seek 0.81ml F*,'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*>'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*U  +22 h F*[% h %n ."MFBPCR"\AS.seek 0.82ml F*Z'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*`  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*M  +65 h  F*h% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *_'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Y  +32 h  ."MFBPCR"\ATV.dial :air3 F*O % +87 h % F*t% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*X% h % ."MFBPCR"\AS.seek 0.81ml F*m'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*5 % +22 h % ."MFBPCR"delay 3 seconds +300 F*r% h %> ."MFBPCR"\AS.seek 0.835ml F*A'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*#  +22 h F*;% h %m ."MFBPCR"\AS.seek 0.82ml F*\\'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*V  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *8'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*n' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *B'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *!'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0121h.pcr #Building PCR Slug from elutionWater with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*|  +11 h  F*w% h %V ."MFBPCR"\AS.seek 0.5ml F*u'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *]'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*k  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*z  +21 h ."MFBPCR"delay 2 seconds +200 F*l% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*S I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F* % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*j% h % ."MFBPCR"\AS.seek 0.53ml F*N' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*$  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*b'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*y I +107 h I ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*R'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*-% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*^  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*o  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*.'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*p % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0121h.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *i'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*\  +21 h ."MFBPCR"\ATV.dial :air3 F*= % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*"  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*:'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*3% h %> ."MFBPCR"\AS.seek 0.835ml F*&'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*<  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*('q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*%  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*?% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *T'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*v' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *1'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*4  +32 h  ."MFBPCR"\ATV.dial :air3 F*D % +86 h % F*G% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *9' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*F% h % ."MFBPCR"\AS.seek 0.81ml F*2'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*) % +22 h % ."MFBPCR"delay 3 seconds +300 F*d% h %> ."MFBPCR"\AS.seek 0.835ml F*7'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*L  +22 h F*Q% h %m ."MFBPCR"\AS.seek 0.82ml F*E'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*I  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *e'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*q' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *M'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0123h.pcr #Building PCR Slug from elutionWater with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*h  +43 h  ."MFBPCR"\ARV.dial :carrier F*Z  +10 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*c n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *_'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b */'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Y  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*O  +22 h ."MFBPCR"delay 2 seconds +200 F*t% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *f'Fj @CET82803.21 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*X% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*5  +22 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*r% h % ."MFBPCR"\AS.seek 0.53ml F*m' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*#  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*;% h % ."MFBPCR"\AS.seek 0.536ml F*A'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*V  +107 h  ."MFBPCR"delay 7 seconds +700 F*\\% h %< ."MFBPCR"\AS.seek 0.594ml F*8'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*n I +108 h I ."MFBPCR"delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*J  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*B' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*~  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*H% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*|  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*w % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0123h.pcr F*!% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *u'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*+ % +86 h % F*]% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *k'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*z  +22 h F*l% h %Z ."MFBPCR"\AS.seek 0.81ml F*x'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*S % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*j% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*$  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*N% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*-  +32 h  ."MFBPCR"\ATV.dial :air3 F*R % +86 h % F*^% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *o' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*p % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*.'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*\  +22 h F*=% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*i  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *"'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *&'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0202h.pcr #Building PCR Slug from elutionWater with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*?  +43 h  ."MFBPCR"\ARV.dial :carrier F*(  +11 h  F*T% h %V ."MFBPCR"\AS.seek 0.5ml F*v'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*} n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *1'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b **'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*4  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*D  +21 h ."MFBPCR"delay 2 seconds +200 F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*{ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*F% h %Fj ."MFBPCR"\AS.seek 0.524ml F*9' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*) n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.53ml F*2' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*L  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.536ml F*7'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*I  +108 h  ."MFBPCR"delay 7 seconds +700 F*E% h %< ."MFBPCR"\AS.seek 0.594ml F*e'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*q I +108 h I ."MFBPCR"delay 2 seconds +200 F*W% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +107 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*,% h %\  ."MFBPCR"\AS.seek 0.65ml F*U' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*[  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*>  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*`% h % \\ ."MFBPCR"\AS.seek 0.326ml F*h'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*Z  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0202h.pcr F*M% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*c  +22 h ."MFBPCR"\ATV.dial :air3 F*g % +87 h % F*_% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Y'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*O  +22 h F*t% h %Z ."MFBPCR"\AS.seek 0.81ml F*/'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*X% h %> ."MFBPCR"\AS.seek 0.835ml F*f'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*5  +21 h F*r% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*#  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*;  +65 h  F*m% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *V'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*\\' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*~% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*H  +22 h F*B% h % ."MFBPCR"\AS.seek 0.81ml F*|'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*w % +21 h % ."MFBPCR"delay 3 seconds +300 F*!% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*+% h %m ."MFBPCR"\AS.seek 0.82ml F*]'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*u  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *z'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*l' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0204h.pcr #Building PCR Slug from elutionWater with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*N  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F* '(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*y n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *b'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*-  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*R  +22 h ."MFBPCR"delay 2 seconds +200 F*^% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*o' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*p I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*\ % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*=% h % ."MFBPCR"\AS.seek 0.536ml F*.'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*i  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*"'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F* I +108 h I ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*3  +107 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*:% h %\  ."MFBPCR"\AS.seek 0.65ml F*<' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*&  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*%% h % \\ ."MFBPCR"\AS.seek 0.326ml F*?'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*(  +42 h  ."MFBPCR"\ASV.dial :PCRlock F*T % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0204h.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *v'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*}  +22 h ."MFBPCR"\ATV.dial :air3 F*C % +87 h % F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *4'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*D  +21 h F*G% h %Z ."MFBPCR"\AS.seek 0.81ml F**'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*{ % +22 h % ."MFBPCR"delay 3 seconds +300 F*F% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +21 h F*d% h %n ."MFBPCR"\AS.seek 0.82ml F*9'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*L  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*Q  +65 h  F*2% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *I'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*E' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *7'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*,  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*[% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *>' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*`  +22 h F*U% h % ."MFBPCR"\AS.seek 0.81ml F*Z'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*M% h %> ."MFBPCR"\AS.seek 0.835ml F*h'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*c  +22 h F*g% h %m ."MFBPCR"\AS.seek 0.82ml F*_'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *O'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*t' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *f'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *;'V +5059 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt270jan0206h.pcr #Building PCR Slug from elutionWater with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*m  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*V% h %V ."MFBPCR"\AS.seek 0.5ml F*\\'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*n n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *s'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *8'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *J'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*H n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*B% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*w  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*!% h % ."MFBPCR"\AS.seek 0.53ml F*|' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*+% h % ."MFBPCR"\AS.seek 0.536ml F* '< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*u  +22 h  ."MFBPCR"delay 7 seconds +700 F*]% h %< ."MFBPCR"\AS.seek 0.594ml F*z'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*l I +107 h I ."MFBPCR"delay 2 seconds +200 F*k% h %\  ."MFBPCR"\AS.seek 0.6ml F*S'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*x% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*j  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*$% h % \\ ."MFBPCR"\AS.seek 0.326ml F*N'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt270jan0206h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*y  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *-'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*R  +22 h F*^% h %Z ."MFBPCR"\AS.seek 0.81ml F*b'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*p  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*o'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*\  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*=  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *i'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *3'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*:  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *&' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*%  +22 h F*<% h % ."MFBPCR"\AS.seek 0.81ml F*('> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*?'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*}  +22 h F*C% h %m ."MFBPCR"\AS.seek 0.82ml F*1'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*v  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *D'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *d' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Q'V +5060 h 'V #t\Asleep after completing 18tun assays .GoodNews.suppressMail "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:17:53, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun assays" #\Mission runtime was 4:27.98885 #-> exit #m\EXIT @1533802887.48CEST @1533968275.93CEST = =Pprocessing =hcore =Ccollection =FMFB =msimfastScheduler =tsimfast =Mmanipulator =Ssampler =Astorage @CET0.00 #t\Copyright 2017 MBARI\ /home/esp/esp2/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @refix37 : @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT:\ @name@ "Can" ;3\\0\\0 .t\Sleepy.power :dwarf,:ON .\Sleepy.power :raw,:ON S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X $bacmfb #-> pucks #-> ESP.ready! C'% M&% P%% h %p %3 ."rdySampler"Intake.select :close M&% h %q ."rdySampler"Exhaust.select :close ."rdyProcessing"\PSV.dial :air h %3 ."rdySampler"\CTV.dial :air P%  .t\Forearm.reconfigure LooseForearm C'  .t\Forearm.home.seek :retract M&#@2^d^2b &'\ +58 h '\  ."closeHand"Hand.close .tElbow.jog 1500 M&/ &* +17 h ."rdySampler"\CSV.dial :waste1 C' +27 h *k .tElbow.reconfigure LooseElbow .Elbow.home.seek :clear M&$ &.,(#b &(d +47 h (d +  ."rdyProcessing"\PS.reconfigure PSconfig .\PS.home.seek 0ml P%#A d`xb %' +38 h ."rdySampler"\SSV.dial :spit,avoiding: :exhaust S) h  S)  h ."readyCS"\CS.reconfigure CSfast ."rdySampler"\SS.reconfigure SSconfig ."readyCS"\CS.home.seek 0ml C'#d @b ."rdySampler"\SS.home.seek 0ml S)#.0X% h %\  M&?& h &_ A(@& h &  A(A% h %X C'B9 h 9 P%C9 h 9 #t-> mfbpcr !o:NameError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:204:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:709:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesgu:NameError::message@undefined local variable or method `mfbpcr' for main:Object: name: mfbpcr #-> bacPCRwcr #Not disinfecting INTAKE in Lab #\Starting bacmfb #pcrBAC sampling at most 1000ml, wcr at most 1000ml .BACfans.suppressMail "Started bacmfb in tube 2 at 08:19:58CEST11-Aug-18\\n{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@08:18:05, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC sampling at most 1000ml, wcr at most 1000ml" #Loading sh1 puck from tube 2 into Collection Clamp F*D% C'E& h %&K #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe .tHand.open ."readyMFB"\AFV.select :holdingCoil M&F1 F* +6 *G% h %&K ."readyMFB"\ATV.dial :waste2 F*H % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *I' +15 h 1 M&J% h %\  A(K% h %X A(L. h ., A(M& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (N& h &  M&O& h &_ .tElbow.reconfigure TightElbow .Elbow.seek 2 M&$&.,(\ #b &P( +22 h ( M&Q& h & A(R& h &  .t\Forearm.seek 2 + 700 counts M&S' +89 h ' A(T& h &  .t\SE.seek 17.71 Pucks below Top Plate A(U'* +87 h '* A(V& h &  M&W& h & .tElbow.seek 2 M&X( +6 h ( M&Y& h & A(Z& h &  .t\Forearm.seek 2 + 300 counts M&['; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &\\/ +150 h / ."downElevator"\SE.seek :down M&]& A(^'X h & M&_% h %; .t\Forearm.seek :retract M&`'\ +87 h 'X ."downElevator"\SE.coast A(5 +4 h '\  .tElbow.seek CC M&a(ӷ +143 h (ӷ M&b& h &ӷ .tElbow.reconfigure LooseElbow M&$ &.,(#b &c& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &d' +91 h ' .t\CC.seek :closed C'e(W +416 h (W .tHand.open M&f1 +150 h 1 M&g% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &h'\ +91 h '\  .t\SC.coast #collectSample 1000ml w/bubble point of 55psi A(6 #t\Priming 7ml .Intake.select :close .Exhaust.select :close .\SS.hold S)i+ +6 h + S)j% h % C'k& h &W "Can" ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +8251 h ' #t\Stroke completed -- waiting for pressure to equalize ... +50 C'?% h %4 S)@% h % S)A% h % .tIntake.select :close S)0 +6 )B% h % .t\SSV.dial :exhaust S)C  +75 h  .tExhaust.select :open S)@ +7 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X' +8250 h ' #t\Stroke completed -- waiting for pressure to equalize ... +50 C'E% h %4 S)G% h % S)D% h % .tIntake.select :close S)0 +7 )F% h % .t\SSV.dial :exhaust S)J  +75 h  .tExhaust.select :open S)@ +6 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X' +8251 h ' #t\Stroke completed -- waiting for pressure to equalize ... +50 C'L% h %4 S)M% h % S)K% h % .tIntake.select :close S)0 +6 )O% h % .t\SSV.dial :exhaust S)Q  +75 h  .tExhaust.select :open S)@ +7 .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X'4 +7 h '4 ."CSpush"\CS.coast C'5 +72 h ' .t\SSV.dial :spit,avoiding: :exhaust S)T +150 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'J9 h 9Td +100 C'G9 h 9kd +100 C'D9 h 9d +100 C'M9 h 9d +100 C'K9 h 9d +100 C'Q9 h 9d +100 C'L9 h 9d +100 C'T9 h 9d +100 C'B9 h 9 d +19 'Rq ."blocking"delay 5 seconds +81 C'>9 h 9 d +100 C'O9 h 96d +100 C'9 h 9Ld +100 C'W9 h 9bd +100 C'Y9 h 9xd +19 ."blocking"\PSR.select :air P% +6 ."blocking"\PS.seek 2.5ml P%N' +75 C'&9 h 9d +100 C'V9 h 9d +100 C')9 h 9d +100 C'H9 h 9d +100 C'_9 h 9d +100 C']9 h 9d +100 C'P9 h 9d +100 C'Z9 h 9(d +100 C'#9 h 9>d +100 C'S9 h 9Td +100 C'[9 h 9id +100 C'c9 h 9d +100 C'^9 h 9d +100 C'R9 h 9d +100 C'b9 h 9d +100 C'f9 h 9d +22 ' ."blocking"\PTV.dial :puck .\PSV.dial PTV P%` +76 h ."blocking"\PBV.dial :waste1 P%j  +2 C'k9 h 9d +98  P%$% h % ."blocking"\PS.seek 2ml P%!' +2 C'g9 h 9  d +100 C'm9 h 9 d +100 C'i9 h 9 *d +100 C'p9 h 9 ?d +100 C'U9 h 9 Td +100 C's9 h 9 j d +83 ' ."blocking"delay 3 seconds +17 C'o9 h 9 $d +100 C'a9 h 9 (d +100 C'v9 h 9 ,d +83 ."blocking"\PBV.dial between PSV and waste1 P%r * +17 C'y9 h 9 0d +8 * ."blocking"\PS.seek 0.25ml P%z' +92 C'w9 h 9 4d +100 C'h9 h 9 8d +100 C'x9 h 9 & h &W #t\Storing sh1 puck in Collection Clamp to tube 1 M&O% h %\  A(% h %X A(W. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (Y( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (&& h & M&V& h &w M&)% h %\  .tElbow.reconfigure TightElbow .Elbow.seek 1 M&$&.,(\ #b &<( +236 h ( M&H& h & A(_& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &]' +90 h ' A(P& h & .t\SE.seek 1.71 Pucks below Top Plate A(Z': +244 h ': M&#& h & A(S& h & .tElbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &['Y +25 h 'Y .tElbow.hold M&c, +7 h , .tElbow.reconfigure TightElbow A(^- M&$&.,(\ #b h -+: .t\SE.seek 2.04 Pucks below Top Plate A(R' +26 h ' M&b% h %Y A(f& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:602:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:600:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:144:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:449:in `process'"2/home/esp/esp2/lib/msg.rb:204:in `initialize'",/home/esp/esp2/lib/msg.rb:201:in `loop'"2/home/esp/esp2/lib/msg.rb:201:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:709:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&`'υ +21 h 'υ M&k& h & A(j& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &$' +22 h ' .t\SE.seek 3.04 Pucks below Top Plate A(g' +36 h ' M&m& h & M&i% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &N'\ +89 h '\  .tElbow.seek CC M&p(ӷ +143 h (ӷ M&U& h &ӷ .tElbow.reconfigure LooseElbow M&$ &.,(#b &s& h &ӷ .t\Forearm.seek CC M&o' +91 h ' .tHand.close M&a/ +150 h / .t\CC.seek :open C'v( +416 h ( A(y& h & A(r& h & M&w& h &ӷ M&h% h % .t\Forearm.seek :retract M&x'\ +91 h '\  .tElbow.reconfigure TightElbow .Elbow.seek 1 M&$&.,(\ #b &!( +142 h ( M&|& h & A(& h & .tElbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &'Y +93 h 'Y .tElbow.hold M&~, +6 h , .tElbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &n1 +150 h 1 M&q% h %Y A(t& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:602:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:600:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:144:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:449:in `process'"2/home/esp/esp2/lib/msg.rb:204:in `initialize'",/home/esp/esp2/lib/msg.rb:201:in `loop'"2/home/esp/esp2/lib/msg.rb:201:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:709:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&'υ +20 h 'υ M&u& h & A(d& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &\ ' +23 h ' A({% h % ."downElevator"\SE.seek :down A( 'X +100 (I% h %1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +89 h '\  +42 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C' @ +2 A(6 +17 h @ .t\CS.seek 10ml C'}' +7 h ' .t\CSV.dial between air and CTV C' ` +18 h ` .t\AIV.dial :bypass C'- { +73 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .tElbow.seek FlushPuck::Garage M&(- +166 h (- M&& h &- .tElbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&& h &- M&"% h %η .t\Forearm.seek :retract M& '\ +91 h '\  .tElbow.reconfigure TightElbow .Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&& h &ӷ .tElbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'z(W +416 h (W C'& h &W .tHand.open M&(1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &*'\ +91 h '\  .t\AWV.select :air C' +6 .t\AIV.dial :bypass .\PSV.dial PSR P% +38 h .t\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%+  +38 h  .t\PS.seek 0ml P%%' +1597 h ' .t\PSV.dial PSR P%0 +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%'' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%3  C'4  h +75 .t\PSV.dial :mixloop P%, +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%  +151 h .t\PS.seek 0ml P%6' +2609 h ' .t\PSV.dial :air P%2  +38 h  .t\PS.seek 2.5ml P%/' +2610 h ' .t\PSV.dial :mixloop P%7 +37 h .t\PS.seek 0ml P%.' +2610 h ' .t\PSV.dial :air P%:  +38 h  .t\PS.seek 2.5ml P%1' +2609 h ' .t\PSV.dial :mixloop P%X +38 h .t\PS.seek 0ml P%' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&?& h &ӷ .tElbow.reconfigure TightElbow .Elbow.seek CC M&$&.,(\ #b &=(ӷ +6 h (ӷ M&;& h &ӷ .tElbow.reconfigure LooseElbow M&$ &.,(#b &A& h &ӷ .t\Forearm.seek CC M&E' +91 h ' .tHand.close M&C/ +150 h / .t\CC.seek :open C'@( +416 h ( M&F& h &ӷ M&J% h % .t\Forearm.seek :retract M&G'\ +91 h '\  .tElbow.reconfigure TightElbow .Elbow.seek :garage M&$&.,(\ #b &5(- +264 h (- M&M& h &- .tElbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &D'η +91 h 'η .tHand.open M&L1 +150 h 1 M&Q% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &B'\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C'>& h & M&O% h %\  A(% h %X A(W. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (&( +201 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (V& h &  M&)& h &- M&H% h %\  .tElbow.reconfigure TightElbow .Elbow.seek 2 M&$&.,(\ #b &_( +166 h ( M&l& h & A(P& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(#& h &  .t\SE.seek 15.71 Pucks below Top Plate A(S'L +106 h 'L A(^& h &  M&b& h & .tElbow.seek 2 M&f( +7 h ( M&k& h & A(j& h &  .t\Forearm.seek 2 + 300 counts M&m'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &i/ +150 h / ."downElevator"\SE.seek :down M&]& h & A(<'X M&Z% h %; .t\Forearm.seek :retract M&['\ +91 h '\  .tElbow.seek CC M&U(ӷ +16 h 'X ."downElevator"\SE.coast A(5 +127 h (ӷ M&s& h &ӷ .tElbow.reconfigure LooseElbow M&$ &.,(#b &`& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &R' +91 h ' .t\CC.seek :closed C'$(W +416 h (W .tHand.open M&c1 +150 h 1 M&a% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &N'\ +90 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'e  +151 h  .t\CSV.dial CTV C'y  +19 h  .t\CS.seek 5ml C'r'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'w' +735 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'h'4 +734 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%|  +38 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P%o +37 h .tdelay 3 seconds +300 .\PSV.dial :air P%p  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%n +38 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%q  +150 h  .t\PS.seek :empty P%t' +2610 h ' .t\CBV.dial :waste1 C'u @ +38 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'd  +37 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'{ @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'x  +38 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'! ` +18 h ` .t\CSV.dial :air C'I @ +38 h @ .t\CS.seek 10ml C'' +2647 h ' .t\CSV.dial CTV C'  +38 h  .t\CS.seek 0ml C''4 +2647 h '4 .t\CBV.dial :waste1 C' @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'  +151 h C'g& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&-% h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (& h & M&\ & h &ӷ M&% h %\  .tElbow.reconfigure TightElbow .Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M& & h & A(Y& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &T' +90 h ' A(~& h & .t\SE.seek 2.71 Pucks below Top Plate A(') +234 h ') M&& h & A("& h & .tElbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b & 'Y +26 h 'Y .tElbow.hold M&, +6 h , .tElbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+) .t\SE.seek 3.04 Pucks below Top Plate A(\\' +27 h ' M&% h %Y A( & h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:602:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:600:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:144:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:449:in `process'"2/home/esp/esp2/lib/msg.rb:204:in `initialize'",/home/esp/esp2/lib/msg.rb:201:in `loop'"2/home/esp/esp2/lib/msg.rb:201:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:709:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &v'υ +20 h 'υ M&& h & A((& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +23 h ' .t\SE.seek 4.04 Pucks below Top Plate A(' +36 h ' M&& h & M&8% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &+'\ +89 h '\  .tElbow.seek CC M&(ӷ +143 h (ӷ M&0& h &ӷ .tElbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.seek CC M&3' +91 h ' .tHand.close M&4/ +150 h / .t\CC.seek :open C',( +416 h ( A(& h & A('& h & M&2& h &ӷ M&6% h % .t\Forearm.seek :retract M&7'\ +90 h '\  .tElbow.reconfigure TightElbow .Elbow.seek 1 M&$&.,(\ #b &/( +143 h ( M&:& h & A(.& h & .tElbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &X'Y +92 h 'Y .tElbow.hold M&1, +7 h , .tElbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &?1 +150 h 1 M&;% h %Y A(A& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:602:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:600:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:144:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:449:in `process'"2/home/esp/esp2/lib/msg.rb:204:in `initialize'",/home/esp/esp2/lib/msg.rb:201:in `loop'"2/home/esp/esp2/lib/msg.rb:201:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:709:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&C'υ +20 h 'υ M&& h & A(*& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &z' +22 h ' A(F% h % ."downElevator"\SE.seek :down A(J'X +100 (E% h %  M&M% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +90 h '\  +31 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%=  +2 A(6 +111 h  P%L% h % .t\PS.seek 0.499ml P%Q' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P%G +37 h .t\PRV.dial :air P%>  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%O  +38 h .t\CBV.dial AIV C'  +37 h .t\PRV.dial between CBV and air P%W  +19 h  .t\PRV.dial :air P%V  +19 h  P%)% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %H' +250 h ' .t\PRV.dial :waste2 P%D @ +75 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %l' +688 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'P& h & .tHand.open M +2 h 1 M&5& h & M&^% h %\  .tElbow.seek FlushPuck::Garage M&b(- +166 h (- M&k& h &- .tElbow.reconfigure LooseElbow M&$ &.,(#b &j& h &- .t\Forearm.seek :garage M&B'η +91 h 'η .tHand.close M&i/ +150 h / M&]& h &- M&Z% h %η .t\Forearm.seek :retract M&_'\ +91 h '\  .tElbow.reconfigure TightElbow .Elbow.seek CC M&$&.,(\ #b &(ӷ +264 h (ӷ M&s& h &ӷ .tElbow.reconfigure LooseElbow M&$ &.,(#b &`& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &S' +91 h ' .t\CC.seek :closed C'm(W +416 h (W C'f& h &W .tHand.open M&<1 +150 h 1 M&[% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &@'\ +91 h '\  .t\CTV.dial :air,avoiding: :intake .\CBV.dial :AIV .\AIV.dial :bypass .\PTV.dial :PRV P%c @ +76 h @ .t\PSV.dial :PTV P%a +37 h .t\PRV.dial :CBV P%e  +113 h .tdelay 5 seconds +500 .\PS.reconfigure PSslow1 .\PS.seek 0.5ml P%#A \ `xb %y'8 +5100 h '8 .tdelay 5 seconds +500 .\PRV.dial :diluent P%R @ +38 h @ .tdelay 5 seconds +500 .\PS.seek 1ml P%&'Rq +5091 h 'Rq .tdelay 5 seconds +500 .\PRV.dial :air P%}  +75 h  .tdelay 2 seconds +200 .\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %r' +1598 h ' .tdelay 2 seconds +200 .\PRV.dial :waste2 P%w @ +75 h @ #tGaraging FlushPuck in Collection Clamp M&|& h &ӷ .tElbow.reconfigure TightElbow .Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&o& h &ӷ .tElbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&n' +91 h ' .tHand.close M&q/ +150 h / .t\CC.seek :open C'( +416 h ( M&u& h &ӷ M&d% h % .t\Forearm.seek :retract M&{'\ +91 h '\  .tElbow.reconfigure TightElbow .Elbow.seek :garage M&$&.,(\ #b &x(- +265 h (- M&!& h &- .tElbow.reconfigure LooseElbow M&$ &.,(#b &I& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &h'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  #t\Starting SH2 of pcrBAC using sig2 .\PTV.dial :puck P%  +76 h  .t\PBV.dial :air .\PTV.dial :puck .\PSV.dial PTV .\PBV.dial :waste1 P%g  +100 h  P%-% h % .t\PS.seek 2ml P%' +585 h ' .tdelay 3 seconds +300 .\PBV.dial between PSV and waste1 P% * +25 h * .t\PS.seek 0.25ml P%' +1850 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'\ & h & .tHand.open M&1 +2 h 1 M& & h &- .tElbow.reconfigure TightElbow .Elbow.seek FlushPuck::Garage M&$&.,(\ #b &Y(- +7 h (- M&~& h &- .tElbow.reconfigure LooseElbow M&$ &.,(#b &U& h &- .t\Forearm.seek :garage M&N'η +91 h 'η .tHand.close M&/ +150 h / M&"& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .tElbow.reconfigure TightElbow .Elbow.seek CC M&$&.,(\ #b & (ӷ +264 h (ӷ M&& h &ӷ .tElbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'8(W +416 h (W C'T& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  C'0& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P%% @ +75 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%v  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML P%\\ +38 h .tdelay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%% h % .t\PS.seek 1.75ml P%4'F +1597 h 'F .tdelay 5 seconds +500 .\PS.seek 2.5ml P%+' +838 h ' .t\PSV.dial :air P%$  +38 h  .t\PS.seek 0ml P%' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%' +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%2  +38 h  .tdelay 2 seconds +200 P%6% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %:'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%.  +75 h .tdelay 2 seconds +200 P%3% h %r .t\PS.seek 0.475ml P%' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%?  +37 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%;'N +4991 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%A  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %*' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%F +75 h .t\PTV.dial :PRV,avoiding: :puck P%7 @ +76 h @ .t\PS.seek 0ml P%/' +2609 h ' .t\PSV.dial :air P%E  +76 h  .t\PS.seek 2.5ml P%M' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%X +76 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%C' +2609 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%  +76 h  .t\PS.seek 2.5ml P%=' +2609 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%L +38 h .t\PTV.dial :PRVmixing,avoiding: :puck P%z  +75 h P%% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %J' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C't & +50 h & .t\AWV.select :waste C' +7 P%G% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %>'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C'O { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %' +1313 h ' .t\PSV.dial :air P%W  +37 h  .t\PS.seek 2.5ml P%V' +2610 h ' .t\PSV.dial :PML P%) +38 h .t\PS.seek 0ml P%Q' +2609 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +7 'D& h &W #"MFBPCR"Extracting Nucleic Acid from AIV #tBeginning samplePassCa ."MFBPCR"\SPE.hold 2:46:40,55C C'H& ."MFBPCR"\ASV.dial :SPEdelivery F*P @@T h &W .t\AWV.select :air F*#  .t\AIV.dial :bypass .\PSV.dial PSR,via: :waste1 P%5  +22 h  ."MFBPCR"delay 20 seconds +166 h .t\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%^' +1598 h ' .tdelay 5 seconds +230 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*k I +128 h I F*j% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *1'6 +142 .t\PSV.dial :waste1 P%i  +37 h  .t\PS.seek 0ml P%]' +1597 h ' .t\PSV.dial PSR P%Z +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%K' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop,via: :air P%B +188 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%s' +1437 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*`  +43 h  F*b% h %6 ."MFBPCR"\AS.seek 0.329ml F*_'ͯ +130 h ' .t\PSV.dial :air P%,  +38 h  .t\PS.seek 2.5ml P%f' +458 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*< % +21 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *['R +1693 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*c  +22 h  F*a% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *S' +415 h ' .t\PSV.dial :mixloop P%e +38 h .t\PS.seek 0ml P%l' +977 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*R I +43 h I F*y% h % ."MFBPCR"\AS.seek 0.469ml F*}' +1290 h ' .t\PSV.dial :air P%&  +37 h  .t\PS.seek 2.5ml P%w' +2610 h ' .t\PSV.dial :mixloop P%| +38 h .t\PS.seek 0ml P%o' +2609 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'p  +38 h  .t\CSR.select :flush C' +6 .t\CS.seek 4ml C'q'P +1116 h 'P .tdelay 5 seconds +322 h ' ."MFBPCR"delay 5 seconds +178 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'' +315 F*@9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*m n +21 h n ."MFBPCR"\ASV.dial :SPEdelivery F*u% h % ."MFBPCR"\AS.seek 0.041ml F*d'% +1289 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'n  '!  +38 h  +113  .t\CBV.dial :waste1 C'I @ +37 h @ .t\CS.seek 0ml C''4 +2647 h '4 .t\CSV.dial CSR C'  +38 h  .t\CSR.select :flush C' +6 .t\CS.seek 4ml C''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'{' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'g'4 +1175 h '% ."MFBPCR"delay 10 seconds +1000 #rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *-' +266 h '4 C'h& h &W .t\PSV.dial :air P%r  +37 h  .t\PTV.dial :puck,via: :PRV P%  +151 h  #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .tElbow.reconfigure TightElbow .Elbow.seek CC M&$&.,(\ #b &\ (ӷ +7 h (ӷ M&& h &ӷ .tElbow.reconfigure LooseElbow M&$ &.,(#b & & h &ӷ .t\Forearm.seek CC M&~' +91 h ' .tHand.close M&U/ +150 h / .t\CC.seek :open C'( +415 h ( M&"& h &ӷ M&% h % .t\Forearm.seek :retract M&x'\ +91 h '\  .tElbow.reconfigure TightElbow .Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&N& h &- .tElbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &('η +91 h 'η .tHand.open M&Y1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1000ml ."sh2"delay 9:31 #"WCR"Loading wcr puck from tube 2 into Collection Clamp C'T& h & M&% h %\  A(% h %X A(0. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (%( +200 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&v& h &- M&\\% h %\  ."WCR"Elbow.reconfigure TightElbow .Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&& h & A(& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &4' +89 h ' A($& h &  ."WCR"\SE.seek 14.71 Pucks below Top Plate A(+'] +116 h '] A('& h &  M&2& h & ."WCR"Elbow.seek 2 M&6( +7 h ( M&& h & A(.& h &  ."WCR"\Forearm.seek 2 + 300 counts M&3'; +22 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &:/ +150 h / ."downElevator"\SE.seek :down M&?& h & A('X M&A% h %; ."WCR"\Forearm.seek :retract M&'\ +91 h '\  ."WCR"Elbow.seek CC M&;(ӷ +25 h 'X ."downElevator"\SE.coast A(5 +117 h (ӷ M&F& h &ӷ ."WCR"Elbow.reconfigure LooseElbow M&$ &.,(#b &7& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' ."WCR"\CC.seek :closed C'E(W +416 h (W ."WCR"Hand.open M&/1 +150 h 1 M&X% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &M'\ +91 h '\  ."WCR"\SC.coast #collectSample 1000ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Intake.select :close .Exhaust.select :close .\SS.hold S)+ +7 h + S)C% h % C'L& h &W "Can" ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'z  +150 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X' +216 h ' ."WCR"\SSV.dial CBV S)W  +151 h  ."WCR"\CTV.dial :intake C'  +75 h  ."connect"\SSV.dial CBV ."WCR"\CBV.dial SSV #\Allowing 40s for 7.0ml of remaining 7.0ml .Intake.select :open S) +207 C')% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 7ml S)#.0X +37 h ."sh2"\PSR.select :wash P% +7 ."sh2"\PS.seek 1ml P%D'Rq +1091 h 'Rq ."sh2"delay 5 seconds +500 .\PSR.select :air P% +6 ."sh2"\PS.seek 2.5ml P%-' +892 h ' #"WCR"\Stroke completed -- waiting for pressure to equalize ... +50 C'#% h %4 S)5% h % S)Q% h % ."WCR"Intake.select :close S)0 +6 )k% h % ."WCR"\SSV.dial :exhaust S)j  +75 h  ."WCR"Exhaust.select :open S)@ +7 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X% h % S))% h % ."WCR"Intake.select :close S)0 +7 )D% h % ."WCR"\SSV.dial :exhaust S)#  +75 h  ."WCR"Exhaust.select :open S)@ +6 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X' +584 h ' ."sh2"delay 3 seconds +300 .\PBV.dial between PSV and waste1 P%) * +25 h * ."sh2"\PS.seek 0.25ml P%D' +1850 h ' ."sh2"delay 2:00 +1283 h ' #"WCR"\Stroke completed -- waiting for pressure to equalize ... +50 C'#% h %4 S)% h % S)Q% h % ."WCR"Intake.select :close S)0 +6 )k% h % ."WCR"\SSV.dial :exhaust S)5  +76 h  ."WCR"Exhaust.select :open S)@ +6 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X% h %4 S)#% h % S)% h % ."WCR"Intake.select :close S)0 +6 )Q% h % ."WCR"\SSV.dial :exhaust S)k  +75 h  ."WCR"Exhaust.select :open S)@ +7 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X  +43 h  #"MFBPCR"\Swish #1 of 2 F*#% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *'\  +507 h ' ."WCR"Exhaust.select :close S) +6 ."connect"\SSV.dial CBV ."WCR"\CBV.dial SSV S)Q  +75 h  #"WCR"\Allowing 130s for 25.0ml of remaining 225.0ml .Intake.select :open S) +207 C'k% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X% h %\  ."MFBPCR"\AS.seek 0.6ml F*#'\  +611 h ' ."sh2"delay 2 seconds +9 h '\  ."MFBPCR"delay 4 seconds +191 ."sh2"\PSV.dial PTV P%4 +75 h ."sh2"delay 2 seconds +134 ."MFBPCR"\ATV.dial ARV F*J  +22 h  ."MFBPCR"delay 2 seconds +44 ."sh2"\PSV.dial :air P%Q  +75 h  ."sh2"delay 2 seconds +81 ."MFBPCR"\ARV.dial :carrier .delay 2 seconds +119 ."sh2"\PSV.dial PTV P%k +75 h ."sh2"delay 2 seconds +6 F*~% h %\  ."MFBPCR"\AS.seek 0.65ml F**' \\ +194 #"sh2"Break puck bottom vacuum .\PBV.dial :air P%  +51 h  ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%Z @ +38 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%X +37 h ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%% @ +38 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%) +38 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%j  +37 h  ."sh2"\PS.seek 0ml P%5' +1180 h ' #"WCR"\Stroke completed -- waiting for pressure to equalize ... +50 C't% h %4 S)-% h % S)]% h % ."WCR"Intake.select :close S)0 +7 )V% h % ."WCR"\SSV.dial :exhaust S)s  +75 h  ."WCR"Exhaust.select :open S)@ +6 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X @ +38 h @ ."sh2"\PBV.dial PSV P% U +25 h U ."sh2"\PS.seek 2.5ml P%4' +2610 h ' ."sh2"delay 3 seconds +300 .\PSR.select :air .\PSV.dial PSR P%J +38 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%Q  +37 h  ."sh2"\PS.reconfigure PSfast .\PS.seek 0ml P%#A ,`xb %k' +1047 h ' #"sh2"Release puck top pressure .\PSV.dial :air P%~  +20 ."WCR"\CTV.dial :air,avoiding: :intake C'  +93 h  ."sh2"\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %#' +58 h #"WCR"\Fix #1 of 2 (with rnal) for 2:00 #\CS Pulling 2ml of rnal .\CSV.dial CSR C'Z  +37 h  ."WCR"\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'X' +606 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +6 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '%' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C') @ +76 h @ +50 S)j% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C't% h % ."WCR"\CSV.dial between air and CTV C'- ` +56 h ` ."WCR"\CS.seek 7.288ml C']' +304 h ' ."sh2"delay 2 seconds +178 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'V  's  +19 h  +3 ."sh2"\PSV.dial PTV P%L +75 h ."sh2"delay 2 seconds +53 h  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ''4 +147 ."sh2"\PSV.dial :air P%[  +76 h  ."sh2"delay 2 seconds +200 .\PSV.dial PTV P%W +75 h ."sh2"delay 2 seconds +200 #Break puck bottom vacuum .\PBV.dial :air P%  +51 h  ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%5 @ +37 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%e +38 h ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%` @ +37 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P%G +38 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%i  +20 h '4 ."WCR"delay 2:00 +18 h  ."sh2"\PS.seek 0ml P%*' +2609 h ' ."sh2"\Sleepy.power :camera,:ON #\Mixing sh2 Substrates .\PSV.dial PSR P%c +38 h ."sh2"\PSR.select :sub1 P%@ +6 %<% h % ."sh2"\PS.seek 0.5ml P%z'8 +585 h '8 ."sh2"delay 2 seconds +200 .\PSR.select :sub2 P% +6 %,% h %8 ."sh2"\PS.seek 1ml P%_'Rq +585 h 'Rq ."sh2"delay 2 seconds +200 .\PSR.select :air P% +7 ."sh2"\PS.seek 2.5ml P%b' +1597 h ' ."sh2"\PTV.dial :puck .\PSV.dial PTV P% +75 h ."sh2"\PBV.dial :waste1 P%m  +101 h  P%B% h % ."sh2"\PS.seek 2ml P%p' +584 h ' ."sh2"delay 3 seconds +300 .\PBV.dial between PSV and waste1 P%? * +25 h * ."sh2"\PS.seek 0.25ml P%n' +1850 h ' ."sh2"delay 7 seconds +700 #recoverReagent out waste1 .\PS.seek 0ml P%1' +332 h ' ."sh2"\PSV.dial PBV P%& @ +38 h @ ."sh2"\PBV.dial PSV P%I U +25 h U ."sh2"\PS.seek 2.5ml P%' +2119 ."WCR"\CBV.dial :waste2b C'}  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'D' +41 h ' ."WCR"delay 1:00 +430 h ' ."sh2"delay 3 seconds +300 .\PSR.select :air .\PSV.dial PSR P%| +38 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%f  +38 h  ."sh2"\PS.reconfigure PSfast .\PS.seek 0ml P%#A ,`xb %{' +1047 h ' #"sh2"Release puck top pressure .\PSV.dial :air P%K  +113 h  ."sh2"\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %l' +2610 h ' ."sh2"delay 2 seconds +200 .\PSV.dial PTV P% +75 h ."sh2"delay 2 seconds +200 .\PSV.dial :air P%R  +75 h  ."sh2"delay 2 seconds +200 .\PSV.dial PTV P% +75 h ."sh2"delay 2 seconds +200 #Break puck bottom vacuum .\PBV.dial :air P%:  +51 h  ."sh2"delay 2 seconds +148 ."WCR"\CTV.dial :air,avoiding: :intake C'  +52 ."sh2"\PSV.dial PBV P%S @ +38 h @ ."sh2"delay 2 seconds +61 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 #\CS Pulling 2ml of rnal .\CSV.dial CSR C'H  +37 h  ."WCR"\CSR.select :rnal C' +7 ."WCR"\CS.seek 2ml C'^' +95 ."sh2"\PSV.dial PSR P%a +37 h ."sh2"delay 2 seconds +200 .\PSV.dial PBV P%r @ +38 h @ ."sh2"delay 2 seconds +200 .\PSV.dial PSR P% +36 h ' ."WCR"delay 5 seconds +2 h ."sh2"delay 2 seconds +200 .\PSV.dial :waste1 P%@  +37 h  ."sh2"\PS.seek 0ml P%Y' +261 ."WCR"\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'q' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C' @ +75 h @ +50 S)"% h % ."WCR"\CBV.dial between CSV and waste2b C'w  +94 h  C'0% h % ."WCR"\CSV.dial between air and CTV C'T ` +57 h ` ."WCR"\CS.seek 7.288ml C'' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'  '\  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b ' '4 +618 h ' P%U9 h 9h #"sh2"\Processing Puck Heater at 29C before imaging .\PH.coast P%8  h h P%x& h &T #"sh2"Imaging sh2 puck in Processing Clamp while storing it to tube 1 M&(% h %\  A( % h %X A(u. h . ."sh2"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (v( +200 h ( ."sh2"\SC.reconfigure SCloose A($.(\ 1~b (& h & M&$& h &ӷ M&2% h %\  ."sh2"Elbow.reconfigure TightElbow .Elbow.seek 1 M&$&.,(\ #b &\\( +143 h ( M& & h & A(!& h & ."sh2"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &N' +89 h ' A(& h & ."sh2"\SE.seek 3.71 Pucks below Top Plate A('' +224 h ' M&& h & A(d& h & ."sh2"Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."sh2"\Forearm.seek 1 M&#Q@2^d^2b &+'Y +25 h 'Y ."sh2"Elbow.hold M&, +7 h , ."sh2"Elbow.reconfigure TightElbow A(- M&$&.,(\ #b h -+ ."sh2"\SE.seek 4.04 Pucks below Top Plate A(/' +27 h ' M&3% h %Y A(h& h & ."sh2"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:602:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:600:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:144:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:449:in `process'"2/home/esp/esp2/lib/msg.rb:204:in `initialize'",/home/esp/esp2/lib/msg.rb:201:in `loop'"2/home/esp/esp2/lib/msg.rb:201:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:709:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."sh2"\Forearm.seek 1 + 300 counts + M&'υ +21 h 'υ M&7& h & A(g& h & ."sh2"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &.' +22 h ' ."sh2"\SE.seek 5.04 Pucks below Top Plate A(o' +36 h ' M&& h & M&F% h % ."sh2"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &='\ +90 h '\  ."sh2"Elbow.seek PC M&(w +236 h (w M&O& h &w ."sh2"Elbow.reconfigure LooseElbow M&$ &.,(#b &A& h &w ."sh2"\Forearm.seek PC M&' +91 h ' ."sh2"Hand.close M&/ +89 h '4 ."WCR"delay 2:00 +61 h / ."sh2"\PC.seek :open P%( +403 h ( A(& h & M&>& h &w M&% h % ."sh2"\Forearm.seek :retract M&C'\ +91 h '\  ."sh2"Elbow.reconfigure TightElbow .Elbow.seek 1 M&$&.,(\ #b &J( +237 h ( M&Q& h & A(4& h & ."sh2"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &~' +89 h ' ."sh2"Hand.open M&1 +150 h 1 A(Z- h -+ ."sh2"\SE.seek 4.79 Pucks below Top Plate A(k'v +25 h 'v ."sh2"\SE.reconfigure SEseal .\SE.seek 4.09 Pucks below Top Plate A(#QK-0-\ b (' +47 h ' #"sh2"Lights, camera, ACTION! #\Simulating PuckCamera: snap -bin=1,1 160.0 /var/log/esp/hires/pcrBAC70jan0212h1000ml160s.tif .delay 2:50 +2000 #"psFlush"Waiting >20 seconds for sh2 to relinquish FlushPuck +8897 ."WCR"\CBV.dial :waste2b C'X  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b ')' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'j  +43 #"sh2"\Simulating PuckCamera: snap -bin=2,2 40.0 /var/log/esp/pcrBAC70jan0212h1000ml40s.tif .delay 50 seconds +107 h +4893 #"sh2"\Simulating PuckCamera: snap -bin=2,2 -auto /var/log/esp/midres/pcrBAC70jan0212h1000ml.tif .delay 1:50 +11000 .BACfans.suppressMail "110.0 sec auto-exposure",Subject: "pcrBAC70jan0212h1000ml images!" .\Sleepy.power :camera,:OFF A(- h -+ A(t& h & M&-& h & ."sh2"Elbow.seek 1 M&%( +7 h ( M&s& h & A(L& h & ."sh2"\Forearm.seek 1 + 700 counts M&V' +7 h ' ."sh2"\SE.reconfigure SEgently .\SE.seek 4.81 Pucks below Top Plate A(#QK-0-\ b ([' +47 h ' M&]& h & A(W& h & ."sh2"Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."sh2"\Forearm.seek 1 M&#Q@2^d^2b &'Y +25 h 'Y ."sh2"Elbow.hold M&5, +7 h , ."sh2"Elbow.reconfigure TightElbow .\SE.reconfigure SEconfig M&$&.,(\ #b ."sh2"\SE.seek 4.99 Pucks below Top Plate A(#QK-0-\ b (e'm +23 h 'm M&`% h %Y A(G& h & ."sh2"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:602:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:600:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:144:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:449:in `process'"2/home/esp/esp2/lib/msg.rb:204:in `initialize'",/home/esp/esp2/lib/msg.rb:201:in `loop'"2/home/esp/esp2/lib/msg.rb:201:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:709:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."sh2"\Forearm.seek 1 + 300 counts +2 M&i'υ +21 h 'υ M&#& h & A(c& h & ."sh2"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &<' +22 h ' A(*% h %m ."downElevator"\SE.seek :down A(,'X +100 (z% h % M&_% h % ."sh2"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +22 'X ."downElevator"\SE.coast A(5 ."sh2"\SC.coast #"psFlush"Loading FlushPuck into Processing Clamp from FlushPuck::Garage C'm& P%B& h &W & ."psFlush"Hand.open M&b1 +2 A(6 h 1 M&?& h & M&p% h %\  ."psFlush"Elbow.seek FlushPuck::Garage M&n(- +166 h (- M&&& h &- ."psFlush"Elbow.reconfigure LooseElbow M&$ &.,(#b &I& h &- ."psFlush"\Forearm.seek :garage M&1'η +91 h 'η ."psFlush"Hand.close M&}/ +150 h / M&& h &- M&|% h %η ."psFlush"\Forearm.seek :retract M&f'\ +91 h '\  ."psFlush"Elbow.reconfigure TightElbow .Elbow.seek PC M&$&.,(\ #b &(w +114 h (w M&K& h &w ."psFlush"Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &w ."psFlush"\Forearm.reconfigure TightForearm .\Forearm.seek PC M&#Q@2^d^\ b &' +91 h ' ."psFlush"\PC.seek :closed P%R(T +404 h (T P%& h &T ."psFlush"Hand.open M&:1 +150 h 1 M&S% h % ."psFlush"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  #"psFlush"\PS Pulling 0.5ml of killMFB #"WCR"\Storing wcr puck in Collection Clamp to tube 1 ."psFlush"\PSV.dial PSR M&H% P%a h %\  A(D% h %X A(r. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (( +7 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (@& h & M&l& h &w M&^% h %\  ."WCR"Elbow.reconfigure TightElbow .Elbow.seek 1 M&$&.,(\ #b &( +31 h ."psFlush"\PSR.select :killMFB P% +6 ."psFlush"\PS.seek 0.5ml P%"'8 +199 h ( M&w& h & A(0& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &T' +90 h ' A(q& h & ."WCR"\SE.seek 4.71 Pucks below Top Plate A(\ ' +214 h ' M&& h & A(& h & ."WCR"Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &U'Y +25 h 'Y ."WCR"Elbow.hold M&8, +7 h , ."WCR"Elbow.reconfigure TightElbow A(x- M&$&.,(\ #b h -+ ."WCR"\SE.seek 5.04 Pucks below Top Plate A((' +27 h ' M& % h %Y A(u& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:602:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:600:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:144:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:449:in `process'"2/home/esp/esp2/lib/msg.rb:204:in `initialize'",/home/esp/esp2/lib/msg.rb:201:in `loop'"2/home/esp/esp2/lib/msg.rb:201:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:709:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&'υ +21 h 'υ M&$& h & A(2& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b & ' + h '8 ."psFlush"delay 5 seconds +21 h ' ."WCR"\SE.seek 6.04 Pucks below Top Plate A(!' +36 h ' M&& h & M&;% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &M'\ +90 h '\  ."WCR"Elbow.seek CC M&(ӷ +142 h (ӷ M&d& h &ӷ ."WCR"Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ ."WCR"\Forearm.seek CC M&3' +91 h ' ."WCR"Hand.close M&h/ +120 ."psFlush"\PSR.select :air P% +6 ."psFlush"\PS.seek 2.5ml P%7' +24 h / ."WCR"\CC.seek :open C'g( +416 h ( A(& h & A(F& h & M&N& h &ӷ M&\\% h % ."WCR"\Forearm.seek :retract M&''\ +91 h '\  ."WCR"Elbow.reconfigure TightElbow .Elbow.seek 1 M&$&.,(\ #b &+( +143 h ( M&& h & A(/& h & ."WCR"Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &.'Y +92 h 'Y ."WCR"Elbow.hold M&O, +7 h , ."WCR"Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &A1 +150 h 1 M&% h %Y A(& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:602:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:600:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:144:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:449:in `process'"2/home/esp/esp2/lib/msg.rb:204:in `initialize'",/home/esp/esp2/lib/msg.rb:201:in `loop'"2/home/esp/esp2/lib/msg.rb:201:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:709:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&='υ +21 h 'υ M& & h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &>' +22 h ' A(% h % ."downElevator"\SE.seek :down A('X +100 (Q% h % M&4% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +13 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast + A(6 +934 h ' ."psFlush"\PTV.dial :puck .\PSV.dial PTV P% +75 h ."psFlush"\PBV.dial :waste1 P%Z  +101 h  ."psFlush"\PS.seek :empty P%C' +889 #tWaiting >20 seconds for psFlush to relinquish FlushPuck +1720 h ' ."psFlush"\PSV.dial PSR P%o +76 h ."psFlush"\PSR.select :flush P%@ +6 ."psFlush"\PS.seek 1ml P%k'Rq +1094 h 'Rq ."psFlush"\PSR.select :air P% +7 ."psFlush"\PS.seek 2.5ml P%X' +1597 h ' ."psFlush"\PSV.dial PTV P% +75 h ."psFlush"\PTV.dial :puck .\PBV.dial :waste1 .\PS.seek 0ml P%j' +2610 h ' ."psFlush"\PSV.dial PSR P% +75 h ."psFlush"\PSR.select :flush P%@ +6 ."psFlush"\PS.seek 0.5ml P%t'8 +585 h '8 ."psFlush"\PSR.select :air P% +6 ."psFlush"\PS.seek 2.5ml P%-' +2104 h ' ."psFlush"\PSV.dial PTV P%s +75 h ."psFlush"\PBV.dial PSV P%L U +50 h U ."psFlush"\PS.seek 0ml P%]' +2610 h ' ."psFlush"\PSV.dial PBV P%W @ +37 h @ ."psFlush"\PS.seek 2.5ml P%`' +2610 h ' ."psFlush"\PSV.dial :waste1 P%G  +75 h  ."psFlush"\PS.seek :empty P%#' +2610 h ' ."psFlush"\PSV.dial PSR P%c +38 h ."psFlush"\PSR.select :air .\PS.seek 2.5ml P%*' +2609 h ' ."psFlush"\PSV.dial PTV P%z +76 h ."psFlush"\PBV.dial PSV .\PS.seek 0ml P%_' +2609 h ' ."psFlush"\PSV.dial PBV P%J @ +38 h @ ."psFlush"\PS.seek 2.5ml P%~' +2610 h ' ."psFlush"\PSV.dial :waste1 P%  +75 h  ."psFlush"\PS.seek :empty P%V' +2610 h ' ."psFlush"\PSV.dial PSR P%m +37 h ."psFlush"\PSR.select :flush P%@ +7 ."psFlush"\PS.seek 1ml P%B'Rq +1094 h 'Rq ."psFlush"\PSR.select :air P% +6 ."psFlush"\PS.seek 2.5ml P%b' +1597 h ' ."psFlush"\PSV.dial PTV P%? +76 h ."psFlush"\PTV.dial :puck .\PBV.dial :waste1 P%p  +50 h  ."psFlush"\PS.seek 0ml P%%' +2609 h ' ."psFlush"\PSV.dial PSR P%[ +76 h ."psFlush"\PSR.select :flush P%@ +6 ."psFlush"\PS.seek 0.5ml P%'8 +585 h '8 ."psFlush"\PSR.select :air P% +6 ."psFlush"\PS.seek 2.5ml P%i' +2103 h ' ."psFlush"\PSV.dial PTV P%e +76 h ."psFlush"\PBV.dial PSV P%& U +50 h U ."psFlush"\PS.seek 0ml P%I' +2609 h ' ."psFlush"\PSV.dial PBV P%< @ +38 h @ ."psFlush"\PS.seek 2.5ml P%,' +2610 h ' ."psFlush"\PSV.dial :waste1 P%v  +75 h  ."psFlush"\PS.seek :empty P%5' +2610 h ' ."psFlush"\PSV.dial PSR P%} +37 h ."psFlush"\PSR.select :air .\PS.seek 2.5ml P%' +2610 h ' ."psFlush"\PSV.dial PTV P%| +75 h ."psFlush"\PBV.dial PSV .\PS.seek 0ml P%' +2610 h ' ."psFlush"\PSV.dial PBV P%K @ +38 h @ ."psFlush"\PS.seek 2.5ml P%{' +2609 h ' ."psFlush"\PSV.dial :waste1 P%1  +76 h  ."psFlush"\PS.seek :empty P%n' +2609 h ' #tLoading FlushPuck into Collection Clamp from Processing Clamp C'f& h & .tHand.open M&1 +2 h 1 M&& h & M&:% h %\  .tElbow.seek PC M&S(w +237 h (w M&H& h &w .tElbow.reconfigure LooseElbow M&$ &.,(#b &D& h &w .t\Forearm.seek PC M&r' +91 h ' .tHand.close M&@/ +150 h / .t\PC.seek :open P%l( +403 h ( M&^& h &w M&a% h % .t\Forearm.seek :retract M&'\ +91 h '\  .tElbow.reconfigure TightElbow .Elbow.seek CC M&$&.,(\ #b &w(ӷ +336 h (ӷ M&0& h &ӷ .tElbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &q' +91 h ' .t\CC.seek :closed C'Y(W +416 h (W C'& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &x'\ +91 h '\  #tBeginning samplePassD C' & h &W .t\AWV.select :air C' +6 .t\AIV.dial :bypass .\PSV.dial PSR P%u +38 h .t\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%$' +1598 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%2  +37 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P%; +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%T' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV C'  +113 h .t\PSV.dial :mixloop P%\ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%U  +151 h .t\PS.seek 0ml P%d' +2609 h ' .t\PSV.dial PSR P% +113 h .t\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%(  +37 h  .t\PS.seek 0ml P% ' +1598 h ' .t\PSV.dial PSR P%8 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%h' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%M +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%"' +2610 h ' .t\PSV.dial PSR P%E +113 h .t\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%F  +38 h  .t\PS.seek 0ml P%N' +1597 h ' .t\PSV.dial PSR P%\\ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%' +2610 h ' .t\CBV.dial PRV ."connect"\PRV.dial CBV .t\PSV.dial :mixloop P%/ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%3' +2609 h ' .t\PSV.dial PSR P% +113 h .t\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%A' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%  +38 h  .t\PS.seek 0ml P%' +1597 h ' .t\PSV.dial PSR P% +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %''k +2679 h 'k .t\PSV.dial :waste1 P%+  +150 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %Q' +1295 h ' .t\PSV.dial :air P%4  +112 h  .t\PS.seek 2.5ml P%.' +2610 h ' .t\PSV.dial :mixloop P%= +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %!' +5106 h ' .t\PSV.dial :air P%>  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' C'Z& h &W #t\Starting finalPurge C'7& h &W #t\Flushing the Collection Valve::Manifold C'o& h &W C'C% h % #t\CS Pulling 5ml of flush .\CSV.dial CSR C'k  +38 h  .t\CSR.select :flush C' +6 .t\CS.seek 5ml C' 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'X% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'j +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 't'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C's& h &W C'L% h %4 .t\CSV.dial :flush C'-  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'W'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'] @ +38 h @ C'9% h %p .t\CS.seek 7ml C'G'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'`  'c  +38 h  +113  .t\CBV.dial :waste1 C'# @ +37 h @ .t\CS.seek 0ml C'z'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'*& h &W C'J% h %4 .t\CSV.dial :flush C'_  +76 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'~ @ +37 h @ C'm% h %p .t\CS.seek 7ml C'V'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'B  +38 h  .t\CBV.dial :waste2b C'?  +75 h  .t\CS.seek 0ml C'p'4 +766 h '4 #t\Flushing lower manifold's waste1 port #\CS Pulling 4ml of mfbkill .\CSV.dial CSR C'b  +38 h  .t\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '['P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '%' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 C'e @ +75 h @ .t\CS.seek 0ml C'&'4 +910 h '4 #t\Flushing lower manifold's waste2b port #\CS Pulling 4ml of mfbkill .\CSV.dial CSR C'i  +38 h  .t\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '<'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'I' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'v  +38 h  .t\CBV.dial :waste2b C',  +75 h  .t\CS.seek 0ml C'}'4 +909 h '4 #tKilling upper manifold #\CS Pulling 4ml of mfbkill .\CSV.dial CSR C'5  +38 h  .t\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '|'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' .t\CBV.dial CSV ."connect"\CSV.dial CBV C'K  ' +38 h +75 .t\CTV.dial :air C'1  +151 h .t\CS.seek 0ml C'{'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'f'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +506 h '4 #t\Flushing upper manifold C':& h &W C'H% h %4 .t\CSV.dial :flush C'D  +38 h  .t\CS.seek 5ml C'O'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'@ @ +38 h @ C'% h %p .t\CS.seek 7ml C'R'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'^ +113 h .t\CTV.dial :air .\CS.seek 0ml C'a'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'r'0 +506 h '0 .t\CSV.dial :waste C'S +38 h .t\CS.seek 0ml C'0'4 +507 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'g  +37 h  .t\CS.seek 3ml C''0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %0 .t\CS.seek 5ml C' 'p +415 h 'p .t\CSV.dial :air .\CS.seek 0ml C'u'4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'q'0 +506 h '0 .t\CSV.dial :waste C'n +76 h .t\CS.seek 0ml C'2'4 +506 h '4 #t\Flushing lower manifold's waste1 port C'$& h &W C';% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C''p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'\ @ +38 h @ C'U% h %p .t\CS.seek 7ml C'T'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  'd  +37 h  +113  .t\CBV.dial :waste1 C'( @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'8& h &W C' % h %4 .t\CSV.dial :flush C'M  +75 h  .t\CS.seek 5ml C'h'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'E @ +38 h @ C'"% h %p .t\CS.seek 7ml C'F'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'\\  +75 h  .t\CS.seek 0ml C'N'4 +766 h '4 C'/& h &W #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .tElbow.reconfigure TightElbow .Elbow.seek CC M&$&.,(\ #b &(ӷ +7 h (ӷ M&3& h &ӷ .tElbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&A' +90 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&+'\ +91 h '\  .tElbow.reconfigure TightElbow .Elbow.seek :garage M&$&.,(\ #b &'(- +265 h (- M&4& h &- .tElbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &='η +91 h 'η .tHand.open M&.1 +150 h 1 M&>% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &!'\ +91 h '\  #twaiting for MFBPCR to finish +397462 !"PCR"o:#AnalyticalModule::PCRRawSample: @refix37 :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0211h1000ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Z  +22 h ."MFBPCR"\ATV.dial :air3 F*7 % +87 h % F*o% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *C'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*k  +22 h F*)% h %Z ."MFBPCR"\AS.seek 0.81ml F*X'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*j% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*s  +21 h F*L% h %n ."MFBPCR"\AS.seek 0.82ml F*-'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*t  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*]  +65 h  F*9% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *W'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*`' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*_  +31 h  ."MFBPCR"\ATV.dial :air3 F*z % +87 h % F*~% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *m' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*B% h % ."MFBPCR"\AS.seek 0.81ml F*?'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*V % +22 h % ."MFBPCR"delay 3 seconds +300 F*b% h %> ."MFBPCR"\AS.seek 0.835ml F*p'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*[  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*e'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*%  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *i'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*&' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *<'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *,'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *|'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0214h1000ml.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*1  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*{% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *f' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*: n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *H'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*@  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *O'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*^ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*R% h %Fj ."MFBPCR"\AS.seek 0.524ml F*a' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*S % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*r% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*g% h % ."MFBPCR"\AS.seek 0.536ml F*0'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*u% h %\  ."MFBPCR"\AS.seek 0.6ml F*n'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*q  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*$% h %\  ."MFBPCR"\AS.seek 0.65ml F*;' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*2  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*\ % h % \\ ."MFBPCR"\AS.seek 0.326ml F*U'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0214h1000ml.pcr F*d% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *('(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*T  +22 h ."MFBPCR"\ATV.dial :air3 F*8 % +86 h % F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *M'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*E% h %Z ."MFBPCR"\AS.seek 0.81ml F*"'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*\\'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*F  +22 h F*/% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*3  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *w'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*x' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Y'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *4'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*Q  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*.% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *>' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*+  +21 h F*=% h % ."MFBPCR"\AS.seek 0.81ml F*P'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*y% h %> ."MFBPCR"\AS.seek 0.835ml F*Z'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*7  +22 h F*o% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*k  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *)'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*C' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *s' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *9'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0216h1000ml.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*-  +43 h  ."MFBPCR"\ARV.dial :carrier F*W  +11 h  F*`% h %V ."MFBPCR"\AS.seek 0.5ml F*c'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*G n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b **'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *_'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*z  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*~  +21 h ."MFBPCR"delay 2 seconds +200 F*J% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*B  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*m% h %Fj ."MFBPCR"\AS.seek 0.524ml F*V' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*b  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*?% h % ."MFBPCR"\AS.seek 0.53ml F*[' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*p% h % ."MFBPCR"\AS.seek 0.536ml F*%'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*e  +108 h  ."MFBPCR"delay 7 seconds +700 F*i% h %< ."MFBPCR"\AS.seek 0.594ml F*&'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*< n +21 h n ."MFBPCR"delay 2 seconds +200 F*v% h %\  ."MFBPCR"\AS.seek 0.6ml F*,'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*I  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*5% h %\  ."MFBPCR"\AS.seek 0.65ml F*}' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*|  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*K  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*1% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*{  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0216h1000ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *:'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*f  +22 h ."MFBPCR"\ATV.dial :air3 F*H % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *@'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*D% h %Z ."MFBPCR"\AS.seek 0.81ml F*^'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*R % +21 h % ."MFBPCR"delay 3 seconds +300 F*O% h %> ."MFBPCR"\AS.seek 0.835ml F*S'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*r  +21 h F*a% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*g  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*0' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *u' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*n  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*2% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *\ ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*d% h %> ."MFBPCR"\AS.seek 0.835ml F*U'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*T  +21 h F*8% h %m ."MFBPCR"\AS.seek 0.82ml F* 'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*(  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*E' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *M'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *h' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\\'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0218h1000ml.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*w% h %V ."MFBPCR"\AS.seek 0.5ml F*x'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Y' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *A'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*Q  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*.% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *4'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*+ I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*=% h %Fj ."MFBPCR"\AS.seek 0.524ml F*>' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*y% h % ."MFBPCR"\AS.seek 0.53ml F*P' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*7  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*o% h % ."MFBPCR"\AS.seek 0.536ml F*Z'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*k  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*)'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*C n +21 h n ."MFBPCR"delay 2 seconds +200 F* % h %\  ."MFBPCR"\AS.seek 0.6ml F*j'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*X  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.65ml F*L' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*t  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*]% h % \\ ."MFBPCR"\AS.seek 0.326ml F*-'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*W  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*` % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0218h1000ml.pcr F*9% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*G  +22 h ."MFBPCR"\ATV.dial :air3 F*# % +86 h % F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *z'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +22 h F*J% h %Z ."MFBPCR"\AS.seek 0.81ml F*_'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*B % +22 h % ."MFBPCR"delay 3 seconds +300 F*m% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*b  +22 h F*?% h %n ."MFBPCR"\AS.seek 0.82ml F*V'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*p  +64 h  F*[% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *e'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*i' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *&' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *I'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*5  +32 h  ."MFBPCR"\ATV.dial :air3 F*, % +86 h % F*|% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *K' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*1  +21 h F*}% h % ."MFBPCR"\AS.seek 0.81ml F*{'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*f  +22 h F*H% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*:  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*D' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *S'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *g' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0221h1000ml.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*0'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*u n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*n  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *$'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*; n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*\ ' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F* I +21 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*d% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*T % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.536ml F*U'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*(  +107 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*E n +21 h n ."MFBPCR"delay 2 seconds +200 F*M% h %\  ."MFBPCR"\AS.seek 0.6ml F*h'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*"% h %\  ."MFBPCR"\AS.seek 0.65ml F*F' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*/  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*\\  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*3% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*w % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0221h1000ml.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *x'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*Y % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *Q'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*.% h %Z ."MFBPCR"\AS.seek 0.81ml F*A'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*+ % +22 h % ."MFBPCR"delay 3 seconds +300 F*=% h %> ."MFBPCR"\AS.seek 0.835ml F*4'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*y% h %n ."MFBPCR"\AS.seek 0.82ml F*>'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*7  +42 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*o  +65 h  F*P% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *k'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Z'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *)' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*s  +32 h  ."MFBPCR"\ATV.dial :air3 F*j % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *t' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*]  +22 h F*L% h % ."MFBPCR"\AS.seek 0.81ml F*W'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*` % +22 h % ."MFBPCR"delay 3 seconds +300 F*9% h %> ."MFBPCR"\AS.seek 0.835ml F*-'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*G  +22 h F*#% h %m ."MFBPCR"\AS.seek 0.82ml F**'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*c  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *~'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*J' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *?' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *p'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt270jan0223h1000ml.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*[  +43 h  ."MFBPCR"\ARV.dial :carrier F*V  +10 h  F*e% h %V ."MFBPCR"\AS.seek 0.5ml F*i'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*< n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *v'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *&'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*5  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*,  +22 h ."MFBPCR"delay 2 seconds +200 F*|% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *I'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*1 n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*}% h %Fj ."MFBPCR"\AS.seek 0.524ml F*K' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*{' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*f  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*H% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*:  +21 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*D n +21 h n ."MFBPCR"delay 2 seconds +200 F*@% h %\  ."MFBPCR"\AS.seek 0.6ml F*R'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*O  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*^% h %\  ."MFBPCR"\AS.seek 0.65ml F*r' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*a  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*S  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*g% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +42 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 @CET174570.97 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt270jan0223h1000ml.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C .\ASV.dial :SPEdelivery F* @@T *0  +44 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *u'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air1 F*  +150 h F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *n' +424 h ' ."MFBPCR"\ATV.dial :ASV F* n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*2  +42 h  F*q% h % ."MFBPCR"\AS.seek 0.62ml F*;'\ +2034 h '\  ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *$'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b * 'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*d'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*\ % +42 h % F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *8' +424 h ' ."MFBPCR"\ATV.dial :air3 F*% h % ."MFBPCR"\AS.seek 0.53ml F*(' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*U% h % ."MFBPCR"\AS.seek 0.71ml F*E' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*"  +22 h F*h% h % Ű ."MFBPCR"\AS.seek 0.72ml F*/' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*\\ n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*3% h % zG ."MFBPCR"\AS.seek 0.365ml F*F' +7161 h ' ."MFBPCR"\ATV.dial :waste F* n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *w'R +1873 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*x' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*.% h %  ."MFBPCR"\AS.seek 0.604ml F*Q'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*+  +22 h F*=% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*A' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*y  +21 h  ."MFBPCR"\AS.seek 0ml F*4'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *o'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*P % +108 h % F*>% h %V ."MFBPCR"\AS.seek 0.01ml F*k' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*Z% h % ."MFBPCR"\AS.seek 0.58ml F*C' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*)% h % q ."MFBPCR"\AS.seek 0.605ml F*s'\ 8 +525 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*j  +22 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*X' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*] n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*L  +21 h  ."MFBPCR"\AS.seek 0ml F*t'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *`' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *9'V +20132 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*W  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *G' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*# n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *-'^ +15507 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*c n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b **'R +202 h 'R ."MFBPCR"\SPE.coast F*~  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*J'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :ASV F*z n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*B  +43 h  F*m% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *_' +4647 h '  ."MFBPCR"\ASV.dial :wasteD F*b I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *?'V +3704 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*[% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *V': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*e  +65 h  F*p% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *i'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*< n +65 h n F*%% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *v' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*5 I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *,'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *|'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*&  +43 h  F*1% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *}': +2036 h ': ."MFBPCR"\ATV.dial :air2 F*I  +65 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*K n +64 h n F*f% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *H' + +2540 h ' + ."MFBPCR"\ASV.dial :wasteD F*{ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +2898 h 'V ."MFBPCR"\ATV.dial :air2 F*  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*D n +64 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *@'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*O'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*^  +85 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*R % +43 h % F*a% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *S' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*g  +21 h F*r% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19126 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*0% h %0 ."MFBPCR"\AS.seek 0.985ml F*' +526 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.97ml F*u'c +323 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F* I +108 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +6 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *2' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *q'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *n' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *;' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *$'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*  +43 h  F*\ % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *T' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *d' +9988 h ' ."MFBPCR"\ATV.dial AIV F* I +43 h I ."MFBPCR"\AS.seek 0.02ml F*8' +9880 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek 0ml F*U'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*(% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *M'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*  +21 h ."MFBPCR"\ATV.dial :air3 F*E % +43 h % F*"% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *h'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*\\% h %Z ."MFBPCR"\AS.seek 0.81ml F*3'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*/ % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*F'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*w'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*Y  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*.' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *x'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *+' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *='V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*y  +32 h  ."MFBPCR"\ATV.dial :air3 F*A % +87 h % F*4% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *7' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*P  +21 h F*>% h % ."MFBPCR"\AS.seek 0.81ml F*o'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*Z% h %> ."MFBPCR"\AS.seek 0.835ml F*k'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*)% h %m ."MFBPCR"\AS.seek 0.82ml F*C'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*j  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4740 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*s' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *X'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *t' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *G'V +5059 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*~ n +86 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *-'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F** % +108 h % ."MFBPCR"\AS.seek 0ml F*z'V +2547 h 'V #t-> exit #mEXIT @1533968818.66CEST @1533969106.96CEST = =tsimfast =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =msimfastScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 02:42:20CET03-Jan-70 .delayUntil "02:42:20.00CET03-Jan-70" @CET178940.00 #-> pucks #-> quit #m\EXIT @1533969126.36CEST @1533969140.48CEST = =msimfastScheduler =tsimfast =ECan =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @refiH; : @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT:\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 02:42:20CET03-Jan-70 .delayUntil "02:42:20.00CET03-Jan-70" @CET178940.00 #Loading /home/esp/esp2/mission/18tun.rb mission .GoodNews.suppressMail "Starting 18tun w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@08:32:28, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 02:42 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNegNTCphase\\n/home/esp/esp2/mission/18tun.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@08:32:28, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 02:42" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@08:32:32, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNegNTCphase" #\Phase [coreNegNTCphase] #==================> STARTED coreNegNTC (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 1500ml .BACfans.suppressMail "Started mfb in tube 2 at 08:32:37CEST11-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@08:32:32, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 1500ml" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp C'& h %&K & #"readyMFB"Readying the MFB M&% #"readyMFB"Homing MFB Syringe h %\  ."readyMFB"\AFV.select :holdingCoil A(% F* h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& h & A('X M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tskipping prime and sample collection #\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'%& h &W ."airStroke"\CSV.dial :waste S)'% ."airStroke"\CS.seek 0ml h % C'/'4 E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +889 C'I% h %4 S)H% h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S)K +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'39 h 9Td +100 C':9 h 9kd +100 C'.9 h 9d +100 C'@9 h 9d +100 C'A9 h 9d +100 C'89 h 9d +100 C'29 h 9d +100 C'D9 h 9d +100 C'E9 h 9 d +100 C'F9 h 9 d +100 C'=9 h 96d +100 C'>9 h 9Ld +100 C')9 h 9bd +100 C'I9 h 9xd +100 C'H9 h 9d +100 C'K9 h 9d +100 C'G9 h 9d +100 C'J9 h 9d +100 C'N9 h 9d +100 C'O9 h 9d +100 C'R9 h 9d +100 C'M9 h 9(d +100 C'S9 h 9>d +100 C'B9 h 9Td +100 C'Q9 h 9id +100 C'L9 h 9d +100 C'P9 h 9d +100 C'V9 h 9d +100 C'W9 h 9d +100 C'T9 h 9d +100 C'Z9 h 9d +100 C'U9 h 9  d +100 C'[9 h 9 d +100 C'Y9 h 9 *d +100 C'\\9 h 9 ?d +100 C'X9 h 9 Td +100 C']9 h 9 j d +100 C'`9 h 9 $d +100 C'_9 h 9 (d +100 C'b9 h 9 ,d +100 C'c9 h 9 0d +100 C'a9 h 9 4d +100 C'f9 h 9 8d +100 C'g9 h 9 % h %Z ."downElevator"\SE.seek :down A()'X +100 (I% h % M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'G @ + A(6 +17 h @ .t\CS.seek 10ml C'J' +7 h ' .t\CSV.dial between air and CTV C'N ` +19 h ` .t\AIV.dial :bypass C'O { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'R& h & .tHand.open M&M1 +2 h 1 M&S& h & M&B% h %\  .t\Elbow.seek FlushPuck::Garage M&Q(- +166 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.seek :garage M&V'η +91 h 'η .tHand.close M&W/ +150 h / M&T& h &- M&Z% h %η .t\Forearm.seek :retract M&U'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &[(ӷ +264 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &X' +91 h ' .t\CC.seek :closed C'](W +416 h (W C'`& h &W .tHand.open M&_1 +150 h 1 M&b% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%a +151 h P%f% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%g' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%e  +38 h  .t\PS.seek 0ml P%h' +1597 h ' .t\PSV.dial PSR P%^ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%k' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%j  C'm  h +75 .t\PSV.dial :mixloop P%d +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%p  +113 h .t\PS.seek 0ml P%r' +2610 h ' .t\PSV.dial :air P%;  +37 h  .t\PS.seek 2.5ml P%o' +2610 h ' .t\PSV.dial :mixloop P%C +38 h .t\PS.seek 0ml P%t' +2609 h ' .t\PSV.dial :air P%u  +38 h  .t\PS.seek 2.5ml P%x' +2610 h ' .t\PSV.dial :mixloop P%? +37 h .t\PS.seek 0ml P%n' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&i& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +7 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.seek CC M&v' +90 h ' .tHand.close M&~/ +150 h / .t\CC.seek :open C'|( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C' & h & M&\ % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (w& h &  M&z& h &- M&}% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 19.71 Pucks below Top Plate A(' +66 h ' A(& h &  M&#& h & .t\Elbow.seek 2 M&( +7 h ( M&& h & A((& h &  .t\Forearm.seek 2 + 300 counts M&'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A(s'X h & M&,% h %; .t\Forearm.seek :retract M&'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&%(ӷ +142 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &$' +91 h ' .t\CC.seek :closed C'4(W +416 h (W .tHand.open M&1 +150 h 1 M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'0' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'9'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%3' +2609 h ' .t\PSV.dial :mixloop P%: +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%/  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%l +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%A  +151 h  .t\PS.seek :empty P%8' +2610 h ' .t\CBV.dial :waste1 C'2 @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'E  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'F @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'>  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'6 ` +19 h ` .t\CSV.dial :air C'< @ +38 h @ .t\CS.seek 10ml C'I' +2647 h ' .t\CSV.dial CTV C'H  +38 h  .t\CS.seek 0ml C'.'4 +2647 h '4 .t\CBV.dial :waste1 C'D @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'G  +150 h C'!& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&N% h %\  A(O% h %X A(R. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (M( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (S& h & M&B& h &ӷ M&=% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &L( +143 h ( M&P& h & A()& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &1' +89 h ' A(& h & .t\SE.seek -0.29 Pucks below Top Plate A(@'\\ +265 h '\\ M&W& h & A(T& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &Z'Y +25 h 'Y .t\Elbow.hold M&K, +7 h , .t\Elbow.reconfigure TightElbow A(V- h -+\\ M&$&.,(\ #b .t\SE.seek 0.04 Pucks below Top Plate A(Y' +26 h ' M&\\% h %Y A(Q& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &U'υ +21 h 'υ M&&& h & A(`& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &_' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(b' +36 h ' M&a& h & M&f% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +89 h '\  .t\Elbow.seek CC M&e(ӷ +143 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&j/ +150 h / .t\CC.seek :open C'm( +416 h ( A(d& h & A(p& h & M&k& h &ӷ M&;% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &C( +142 h ( M&o& h & A(u& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &t'Y +93 h 'Y .t\Elbow.hold M&?, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &x1 +150 h 1 M&i% h %Y A(q& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &{'υ +21 h 'υ M&~& h & A([& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &c' +22 h ' A(]% h % ."downElevator"\SE.seek :down A('X +100 (% h %S M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast .t\SC.coast A(5 .t\PSV.dial :air P%  +2 A(6 +111 h  P%y% h % .t\PS.seek 0.499ml P% ' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'\  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%  +19 h  P%w% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %z' +250 h ' .t\PRV.dial :waste2 P%} @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&('η +91 h 'η .tHand.close M& / +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +90 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&s1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'|& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%5  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%$' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%J  +38 h  .t\PS.seek 0ml P%7' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%0 +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P%n% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %:'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%/  +75 h .tdelay 2 seconds +200 P%l% h %r .t\PS.seek 0.475ml P%A' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%3  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%2'N +4990 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%E  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%F  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %>' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%6 +76 h .t\PTV.dial :PRV,avoiding: :puck P%< @ +75 h @ .t\PS.seek 0ml P%9' +2610 h ' .t\PSV.dial :air P%H  +75 h  .t\PS.seek 2.5ml P%I' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%D +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%G' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%!  +75 h  .t\PS.seek 2.5ml P%N' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%O +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%R  +76 h P%S% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %B' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'= & +50 h & .t\AWV.select :waste C' +6 P%P% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %)'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %%' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%W' +2609 h ' .t\PSV.dial :PML P%T +38 h .t\PS.seek 0ml P%V' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '\\& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'Q& h &W ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air F*& @@T .t\AIV.dial :bypass F*`  .t\PSV.dial PSR P%a +22 h  ."MFBPCR"delay 20 seconds +91 h P%f% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*L I +129 h I F*@% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *Z'6 +66 .t\PSV.dial :waste1 P%g  +37 h  .t\PS.seek 0ml P%^' +1597 h ' .t\PSV.dial PSR P%U +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Y' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%K' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*j  +22 h ' .t\PSV.dial PSR P%X +21 h  F*4% h %6 ."MFBPCR"\AS.seek 0.329ml F*d'ͯ +92 h P%p% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%k' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*; % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *o'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%u  +38 h  .t\PS.seek 0ml P%h' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*e  +22 h  F*x% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *i' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%q +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%~' +217 ."MFBPCR"\ATV.dial :AIV F*[ I +43 h I F*]% h % ."MFBPCR"\AS.seek 0.469ml F*r' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%C +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P%v +113 h P%t% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%{' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%b  +37 h  .t\PS.seek 0ml P%' +740 h ' ."MFBPCR"delay 5 seconds +500 F*y9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*c n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*M'% +336 h ' .t\PSV.dial PSR P%8 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial PSR P%\ +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%w  +38 h  .t\PS.seek 0ml P% ' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%} +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%z' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %#' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *?'V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%(  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1327 h 'V ."MFBPCR"\ATV.dial :air2 F*'  +42 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *,'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*m I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*s' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %|' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F**'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*5  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*$% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *J'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'0% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'n' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'7  '/  +38 h  +113  .t\CBV.dial :waste1 C'l @ +37 h @ .t\CS.seek 0ml C':'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*3  +21 h ."MFBPCR"delay 3 seconds +300 F*A% h %_ ."MFBPCR"\AS.seek 0.434ml F*E'ݏ +110 h '4 .t\CSV.dial CSR C'F  +38 h  C'2% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'6'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*<  +22 h F*>% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*H'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*9 n +108 h n F*D% h %& ."MFBPCR"\AS.seek 0.092ml F*I'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'!' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'O'4 +2647 h '4 C'R& h &W #tGaraging FlushPuck in Collection Clamp M&S& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +6 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&B' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C')( +416 h ( M&"& h &ӷ M&%% h % .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &W(- +265 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +90 h 'η .tHand.open M&a1 +150 h 1 M&f% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #"WCR"Whole Cell Archive sampling 1500ml #Loading wcr puck from tube 2 into Collection Clamp C'L& h & M&@% h %\  A(g% h %X A( . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (1( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (U& h &  M&^& h &- M&_% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &Y( +166 h ( M&j& h & A(4& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &X' +89 h ' A(p& h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(Z' +77 h ' A(K& h &  M&;& h & ."WCR"\Elbow.seek 2 M&d( +6 h ( M&u& h & A(e& h &  ."WCR"\Forearm.seek 2 + 300 counts M&x'; +23 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &k/ +150 h / ."downElevator"\SE.seek :down A(q'X M&o& h & M&[% h %; ."WCR"\Forearm.seek :retract M&]'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&h(ӷ +142 h (ӷ M&i& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &~' +91 h ' ."WCR"\CC.seek :closed C'v(W +3 ."MFBPCR"\ATV.dial :waste2 F*t % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&b1 +150 h 1 M&{% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*c  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +29 h '\  ."WCR"\SC.coast #collectSample 1500ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)r+ +6 h + S)8% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X% h % ."WCR"Intake.select :close S)0 +6 )E% h % ."WCR"\SSV.dial :exhaust S)9  +75 h  ."WCR"\Exhaust.select :open S)@ +7 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X% h %4 S)E% h % S)9% h % ."WCR"Intake.select :close S)0 +6 )2% h % ."WCR"\SSV.dial :exhaust S)H  +75 h  ."WCR"\Exhaust.select :open S)@ +7 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X% h %X ."MFBPCR"\AS.seek 0.24ml F*E' +194 h ' ."WCR"\Exhaust.select :close S) +7 ."connect"\SSV.dial CBV ."WCR"\CBV.dial SSV S)9  +75 h  #"WCR"\Allowing 130s for 25.0ml of remaining 1000.0ml .Intake.select :open S) +48 h '  ."MFBPCR"delay 3 seconds +158 C'2% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X'< +70 h ' ."WCR"\Exhaust.select :close S) +7 ."connect"\SSV.dial CBV ."WCR"\CBV.dial SSV S)9  +75 h  #"WCR"\Allowing 130s for 25.0ml of remaining 800.0ml .Intake.select :open S) +206 C'n% h %4 #"WCR"Intake @14.7psia, vacuum from 8.2 to 12.2psi .\SS.reconfigure SSinhale.with maxSpeed: 21 .\SS.seek 25ml S)#.0X  +75 h  ."WCR"\Exhaust.select :open S)@ +6 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X  +75 h  ."WCR"\Exhaust.select :open S)@ +6 ."WCR"\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0X& h & M&5& h &ӷ M&6% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &=( +143 h ( M& & h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(R& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A(:'K +254 h 'K M&2& h & A(E& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &\\'Y +25 h 'Y ."WCR"\Elbow.hold M&, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(<- M&$&.,(\ #b h -+K ."WCR"\SE.seek 1.04 Pucks below Top Plate A(H' +26 h ' M&!% h %Y A(J& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&G'υ +21 h 'υ M&Q& h & A(& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &P' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(L' +36 h ' M&a& h & M&^% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &U'\ +89 h '\  ."WCR"\Elbow.seek CC M&@(ӷ +143 h (ӷ M&N& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &4& h &ӷ ."WCR"\Forearm.seek CC M&f' +91 h ' ."WCR"Hand.close M&j/ +150 h / ."WCR"\CC.seek :open C'g( +416 h ( A(S& h & A(u& h & M&B& h &ӷ M&V% h % ."WCR"\Forearm.seek :retract M&%'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &o( +142 h ( M& & h & A(p& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &;'Y +93 h 'Y ."WCR"\Elbow.hold M&`, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &e1 +150 h 1 M&W% h %Y A(K& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&_'υ +20 h 'υ M&Y& h & A([& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &C' +23 h ' A(d% h % ."downElevator"\SE.seek :down A(i'X +100 (q% h %B M&{% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &t'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C']& h & .tHand.open M&c1 + A(6 + h 1 M&Z& h & M&b% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &r& h &- .t\Forearm.seek :garage M&x'η +90 h 'η .tHand.close M&8/ +150 h / M& & h &- M&X% h %η .t\Forearm.seek :retract M&k'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &)(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &O& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C' (W +416 h (W C'& h &W .tHand.open M&}1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &~'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C' & h &W C'% h % .t\CSV.dial CSR C'  +37 h  C'% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'\ 'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 ''% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '#'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'% h %4 .t\CSV.dial :flush C'w  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b 'z'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C'M'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +37 h  +113  .t\CBV.dial :waste1 C' @ +76 h @ .t\CS.seek 0ml C'$'4 +766 h '4 #t\Flushing lower manifold's waste2b port C's& h &W C'% h %4 .t\CSV.dial :flush C'(  +75 h  .t\CS.seek 5ml C'*'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'+  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C'?'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'F  +37 h  C'0% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '3' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 C'. @ +76 h @ .t\CS.seek 0ml C'm'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'9  +38 h  C'A% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'n'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '7' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'l  +38 h  .t\CBV.dial :waste2b C'>  +75 h  .t\CS.seek 0ml C'5'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'6  +37 h  C' % h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'R' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'h 'y  +37 h +76 .t\CTV.dial :air C'2  +150 h .t\CS.seek 0ml C'E'4 +910 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'<'0 +507 h '0 .t\CSV.dial :waste C'! +37 h .t\CS.seek 0ml C'J'4 +507 h '4 #t\Flushing upper manifold C'Q& h &W C'% h %4 .t\CSV.dial :flush C'a  +37 h  .t\CS.seek 5ml C'^'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'=% h %p .t\CS.seek 7ml C':'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'\\ +113 h .t\CTV.dial :air .\CS.seek 0ml C'N'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'4'0 +507 h '0 .t\CSV.dial :waste C'G +37 h .t\CS.seek 0ml C'H'4 +507 h '4 C'P& h &W C'% h %4 .t\CSV.dial :flush C'j  +38 h  .t\CS.seek 3ml C'U'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'v @ +38 h @ C'S% h %0 .t\CS.seek 5ml C'u'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'B'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'V'0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'p'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'f& h &W C'@% h %4 .t\CSV.dial :flush C'e  +37 h  .t\CS.seek 5ml C'W'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'K @ +38 h @ C'Y% h %p .t\CS.seek 7ml C'['i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'd  '%  +38 h  +113  .t\CBV.dial :waste1 C'o @ +37 h @ .t\CS.seek 0ml C'q'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'{& h &W C']% h %4 .t\CSV.dial :flush C'c  +76 h  .t\CS.seek 5ml C'Z'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'b @ +37 h @ C';% h %p .t\CS.seek 7ml C'_'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'L  +38 h  .t\CBV.dial :waste2b C'C  +75 h  .t\CS.seek 0ml C'I'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&r& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &i(ӷ +7 h (ӷ M&D& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &`& h &ӷ .t\Forearm.seek CC M&8' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'X( +416 h ( M&t& h &ӷ M&x% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &O(- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &k& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &g'η +91 h 'η .tHand.open M&1 +150 h 1 M&}% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C' & h & .tHand.open M&1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +6 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &/& h &- .t\Forearm.seek :garage M&''η +91 h 'η .tHand.close M&\ / +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&w& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &#& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'z& h &W .tHand.open M&1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'M& h &W C's% h %4 .t\CSV.dial CSR C'  +37 h  C'(% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '$'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C'* +150 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '+'4 +2010 h '4 #t\Purging external Intake C'& h &W C'% h %4 .t\CSV.dial :flush C'F  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '0' +909 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'? ',  +38 h +38 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'.'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'3  +76 h #t\Flushing upper manifold C'9& h &W C'A% h %4 .t\CSV.dial :flush C'm  +75 h  .t\CS.seek 5ml C'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'l @ +38 h @ C'>% h %p .t\CS.seek 7ml C'7'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'6 +113 h .t\CTV.dial :air .\CS.seek 0ml C' '4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'5'0 +506 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C'y'4 +507 h '4 C'h& h &W C'2% h %4 .t\CSV.dial :flush C'R  +37 h  .t\CS.seek 3ml C'E'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'! @ +37 h @ C'<% h %0 .t\CS.seek 5ml C'Q'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +651 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'a'0 +506 h '0 .t\CSV.dial :waste C'J +76 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&=& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &^(ӷ +7 h (ӷ M&\\& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&N' +91 h ' .tHand.close M&G/ +150 h / .t\CC.seek :open C'4( +416 h ( M&P& h &ӷ M&% h % .t\Forearm.seek :retract M&j'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &H(- +264 h (- M&v& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &S& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &U'η +91 h 'η .tHand.open M&u1 +150 h 1 M&B% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Completed [coreNegNTCphase] begun 02:42:51 #\Extending 18tun until completion of MFBPCR +257348 !"PCR"o:#AnalyticalModule::PCRRawSample: @refiH; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0304h.pcr F*V% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *f'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*@  +21 h ."MFBPCR"\ATV.dial :air3 F*e % +87 h % F*p% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *K'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Y  +22 h F*W% h %Z ."MFBPCR"\AS.seek 0.81ml F*d'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*o% h %> ."MFBPCR"\AS.seek 0.835ml F*['n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*{  +22 h F*]% h %n ."MFBPCR"\AS.seek 0.82ml F*c'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*q  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*b  +65 h  F*;% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *Z'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*L' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *C'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*) % +86 h % F*~% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*t  +22 h F*x% h % ."MFBPCR"\AS.seek 0.81ml F*8'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*k% h %> ."MFBPCR"\AS.seek 0.835ml F*i'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*}% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *g'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\ ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *''V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0306h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*w  +43 h  ."MFBPCR"\ARV.dial :carrier F*#  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*z n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*M  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*s  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *('Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*I% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*$ % +22 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F**% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*F'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*+  +21 h  ."MFBPCR"delay 7 seconds +700 F*,% h %< ."MFBPCR"\AS.seek 0.594ml F*?'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*0% h %\  ."MFBPCR"\AS.seek 0.6ml F*3'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*9  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*A% h %\  ."MFBPCR"\AS.seek 0.65ml F*m' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*.  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*l  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*>% h % \\ ."MFBPCR"\AS.seek 0.326ml F*n'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*6  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*7 % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0306h.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*5  +22 h ."MFBPCR"\ATV.dial :air3 F*h % +86 h % F*2% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *R'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*y  +21 h F*!% h %Z ."MFBPCR"\AS.seek 0.81ml F*<'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*E % +22 h % ."MFBPCR"delay 3 seconds +300 F*Q% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*J  +22 h F*a% h %n ."MFBPCR"\AS.seek 0.82ml F*='q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*\\  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*:  +65 h  F*G% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*S  +32 h  ."MFBPCR"\ATV.dial :air3 F*^ % +87 h % F*u% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *B' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*j  +22 h F*U% h % ."MFBPCR"\AS.seek 0.81ml F*&'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*V % +22 h % ."MFBPCR"delay 3 seconds +300 F*T% h %> ."MFBPCR"\AS.seek 0.835ml F*@'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*e  +22 h F*p% h %m ."MFBPCR"\AS.seek 0.82ml F*f'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*Y  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *W'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*K' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *o' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *b' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *;'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0308h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*c  +43 h  ."MFBPCR"\ARV.dial :carrier F*Z  +11 h  F*L% h %V ."MFBPCR"\AS.seek 0.5ml F*C'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*r n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *D'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b * '(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*)  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*~  +22 h ."MFBPCR"delay 2 seconds +200 F*`% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *t'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*x  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*k  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*8% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*}  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*i% h % ."MFBPCR"\AS.seek 0.536ml F* '< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*/% h %\  ."MFBPCR"\AS.seek 0.6ml F*g'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*\  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*O  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*w% h % \\ ."MFBPCR"\AS.seek 0.326ml F*#'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*' % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0308h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *z'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*X  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*M% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *s'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*I % +22 h % ."MFBPCR"delay 3 seconds +300 F*(% h %> ."MFBPCR"\AS.seek 0.835ml F*$'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F**  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*+% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *,'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *A'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*3  +32 h  ."MFBPCR"\ATV.dial :air3 F*. % +86 h % F*l% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *>' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*m  +22 h F*6% h % ."MFBPCR"\AS.seek 0.81ml F*7'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*" % +21 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*n'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*5  +22 h F*h% h %m ."MFBPCR"\AS.seek 0.82ml F*2'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *y'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*!' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *Q'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *a' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *\\' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0311h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*G  +43 h  ."MFBPCR"\ARV.dial :carrier F*=  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*P n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*S  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*^  +22 h ."MFBPCR"delay 2 seconds +200 F*u% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *v'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*j I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*U% h %Fj ."MFBPCR"\AS.seek 0.524ml F*B' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*V n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*T% h % ."MFBPCR"\AS.seek 0.53ml F*&' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*e  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*p% h % ."MFBPCR"\AS.seek 0.536ml F*@'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*Y  +107 h  ."MFBPCR"delay 7 seconds +700 F*f% h %< ."MFBPCR"\AS.seek 0.594ml F*W'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*K n +21 h n ."MFBPCR"delay 2 seconds +200 F*%% h %\  ."MFBPCR"\AS.seek 0.6ml F*o'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*d  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*{% h %\  ."MFBPCR"\AS.seek 0.65ml F*]' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*[  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*q  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*b% h % \\ ."MFBPCR"\AS.seek 0.326ml F*c'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*Z  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*L % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0311h.pcr F*;% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *C'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*r  +22 h ."MFBPCR"\ATV.dial :air3 F*_ % +86 h % F*D% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *)'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +21 h F*`% h %Z ."MFBPCR"\AS.seek 0.81ml F* '> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*t'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*k  +22 h F*8% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*}  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*i  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*g % +87 h % F*O% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*' % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*#'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*M'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*z  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *('V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b **'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0313h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*+  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*,% h %V ."MFBPCR"\AS.seek 0.5ml F*F'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*0 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *?'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *9'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*3  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*.  +22 h ."MFBPCR"delay 2 seconds +200 F*l% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *A'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*m n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*6% h %Fj ."MFBPCR"\AS.seek 0.524ml F*>' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*" I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F* % h % ."MFBPCR"\AS.seek 0.53ml F*7' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*5 % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*h% h % ."MFBPCR"\AS.seek 0.536ml F*n'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*2% h %< ."MFBPCR"\AS.seek 0.594ml F*y'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*! n +21 h n ."MFBPCR"delay 2 seconds +200 F*R% h %\  ."MFBPCR"\AS.seek 0.6ml F*E'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*Q  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*<% h %\  ."MFBPCR"\AS.seek 0.65ml F*J' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*a  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*\\% h % \\ ."MFBPCR"\AS.seek 0.326ml F*G'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*=  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0313h.pcr F*:% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*P  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *S'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*^  +22 h F*u% h %Z ."MFBPCR"\AS.seek 0.81ml F*N'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +22 h % ."MFBPCR"delay 3 seconds +300 F*U% h %> ."MFBPCR"\AS.seek 0.835ml F*v'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*V  +22 h F*T% h %n ."MFBPCR"\AS.seek 0.82ml F*B'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*e  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*p  +64 h  F*&% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *Y'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*f' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *@'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *%'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*{  +32 h  ."MFBPCR"\ATV.dial :air3 F*o % +86 h % F*[% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *q' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*b  +21 h F*]% h % ."MFBPCR"\AS.seek 0.81ml F*Z'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*L % +22 h % ."MFBPCR"delay 3 seconds +300 F*;% h %> ."MFBPCR"\AS.seek 0.835ml F*c'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*r  +22 h F*_% h %m ."MFBPCR"\AS.seek 0.82ml F*D'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*C  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *~'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*`' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *t'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *}' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *i'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt270jan0315h.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b */'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*g  +21 h ."MFBPCR"delay 2 seconds +200 F*O% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *\ 'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*w n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*'  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*X  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*#'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*z  +22 h  ."MFBPCR"delay 7 seconds +700 F*M% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +22 h n ."MFBPCR"delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.6ml F*I'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*(  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F**' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*$  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*+'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*, % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt270jan0315h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F* @@T ."MFBPCR"\ASV.dial :SPEdelivery F*F  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *0'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*  +151 h F*?% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *3' +423 h ' ."MFBPCR"\ATV.dial :ASV F*. n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*l  +43 h  F*9% h % ."MFBPCR"\AS.seek 0.62ml F*m'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*6 n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *A'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *"'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F* '(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*> % +43 h % F*5% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *h' +423 h ' ."MFBPCR"\ATV.dial :air3 F*7% h % ."MFBPCR"\AS.seek 0.53ml F*' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*2  +21 h F*n% h % ."MFBPCR"\AS.seek 0.71ml F*!' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*R % +22 h % ."MFBPCR"delay 3 seconds +300 F*y% h % H ."MFBPCR"\AS.seek 0.735ml F*Q' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*<  +22 h F*E% h % Ű ."MFBPCR"\AS.seek 0.72ml F*a' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*\\% h % zG ."MFBPCR"\AS.seek 0.365ml F*J' +7161 h ' ."MFBPCR"\ATV.dial :waste F*= n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F*: % +43 h % F*G% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *P' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*^ % +22 h % ."MFBPCR"delay 3 seconds +300 F*u% h %  ."MFBPCR"\AS.seek 0.604ml F*S'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*j  +21 h F*U% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*N' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*V n +86 h n ."MFBPCR"\ASV.dial :wasteR F*T  +22 h  ."MFBPCR"\AS.seek 0ml F*v'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *p'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*& % +108 h % F*B% h %V ."MFBPCR"\AS.seek 0.01ml F*Y' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +22 h F*@% h % ."MFBPCR"\AS.seek 0.58ml F*K' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*W% h % q ."MFBPCR"\AS.seek 0.605ml F*{'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +21 h F*[% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*d' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*b n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*]  +21 h  ."MFBPCR"\AS.seek 0ml F*q'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *;'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*Z  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *r' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*_ n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *c'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*C n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *D'R +202 h 'R ."MFBPCR"\SPE.coast F*~  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*`'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*) n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*x  +43 h  F* % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b * ' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*k I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *8'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *t'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*}  +43 h  F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*i% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F* % h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b */' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *g'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +43 h  F*w% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*\  +64 h  F*'% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F* n +65 h n F*X% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *z'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*M  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *#': +3043 h ': ."MFBPCR"\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *s'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F* n +151 h n ."MFBPCR"\AS.seek 0ml F*('V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*I % +43 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *$' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F**% h % ."MFBPCR"\AS.seek 0.96ml F*'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*, % +22 h % ."MFBPCR"delay 3 seconds +300 F*F% h %0 ."MFBPCR"\AS.seek 0.985ml F*+' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*?% h % ."MFBPCR"\AS.seek 0.97ml F*0'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*. I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *9'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *6'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *A'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*"  +43 h  F*>% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *5' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b * ' +9988 h ' ."MFBPCR"\ATV.dial AIV F*7 I +43 h I ."MFBPCR"\AS.seek 0.02ml F*h' +9880 h ' ."MFBPCR"\ATV.dial :waste F*2 n +129 h n ."MFBPCR"\AS.seek 0ml F*n'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *R'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*y  +22 h ."MFBPCR"\ATV.dial :air3 F*! % +43 h % F*<% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *E'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*Q  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*\\'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*a % +22 h % ."MFBPCR"delay 3 seconds +300 F*=% h %> ."MFBPCR"\AS.seek 0.835ml F*J'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*:  +22 h F*G% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*P% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *^'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *S' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *V'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*T  +32 h  ."MFBPCR"\ATV.dial :air3 F*N % +86 h % F*v% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *e' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +21 h F*B% h % ."MFBPCR"\AS.seek 0.81ml F*p'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*f % +22 h % ."MFBPCR"delay 3 seconds +300 F*@% h %> ."MFBPCR"\AS.seek 0.835ml F*Y'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*%  +22 h F*W% h %m ."MFBPCR"\AS.seek 0.82ml F*K'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*o  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *['R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*{' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *b'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *d'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *r'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*~ n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *c'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*D % +108 h % ."MFBPCR"\AS.seek 0ml F*)'V +2547 h 'V #"MFBPCR"\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0319h.pcr #Building PCR Slug from elutionWater with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*x  +64 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*`% h %V ."MFBPCR"\AS.seek 0.5ml F*k'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*8 n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*t  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*i% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*  +21 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*/% h % ."MFBPCR"\AS.seek 0.53ml F*g' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +22 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*w% h % ."MFBPCR"\AS.seek 0.536ml F*O'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*\  +108 h  ."MFBPCR"delay 7 seconds +700 F*'% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F* I +108 h I ."MFBPCR"delay 2 seconds +200 F*X% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +107 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*M' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*z  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*#% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*s  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0319h.pcr F*I% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*(  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F**% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *$'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*,  +21 h F*F% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*?% h %> ."MFBPCR"\AS.seek 0.835ml F*+'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*.  +21 h F*0% h %n ."MFBPCR"\AS.seek 0.82ml F*l'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*9  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*3  +65 h  F*6% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *m'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*"' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *>'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *A' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *5'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*2  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*h% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *n' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*y  +22 h F*!% h % ."MFBPCR"\AS.seek 0.81ml F*<'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*R % +22 h % ."MFBPCR"delay 3 seconds +300 F*Q% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*E  +22 h F*a% h %m ."MFBPCR"\AS.seek 0.82ml F*='q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*\\  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *:'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*G' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *P' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *u'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *j'V +5059 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0321h.pcr #Building PCR Slug from elutionWater with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*U  +43 h  ."MFBPCR"\ARV.dial :carrier F*S  +11 h  F*T% h %V ."MFBPCR"\AS.seek 0.5ml F*N'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *v' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*V n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *&'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *B'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*e  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*f  +22 h ."MFBPCR"delay 2 seconds +200 F*@% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *p'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*% I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*W% h %Fj ."MFBPCR"\AS.seek 0.524ml F*Y' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*o % +21 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*K% h % ."MFBPCR"\AS.seek 0.53ml F*[' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*{  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*b% h % ."MFBPCR"\AS.seek 0.536ml F*]'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*d  +22 h  ."MFBPCR"delay 7 seconds +700 F*q% h %< ."MFBPCR"\AS.seek 0.594ml F*L'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*Z I +107 h I ."MFBPCR"delay 2 seconds +200 F*;% h %\  ."MFBPCR"\AS.seek 0.6ml F*_'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*r  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*~% h %\  ."MFBPCR"\AS.seek 0.65ml F*C' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*D  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*c  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*x% h % \\ ."MFBPCR"\AS.seek 0.326ml F* '{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*`  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*) % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0321h.pcr F*k% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *8'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F*} % +86 h % F*t% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*i  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*/  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*w  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*g  +64 h  F*\ % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *''n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*O' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*z % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *#' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*M  +21 h F*s% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*I% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *,'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*F' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *0' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *3'V +5060 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0323h.pcr #Building PCR Slug from elutionWater with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*6  +43 h  ."MFBPCR"\ARV.dial :carrier F*l  +11 h  F*m% h %V ."MFBPCR"\AS.seek 0.5ml F*"'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *>' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*A n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *5'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *7'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +21 h ."MFBPCR"delay 2 seconds +200 F*h% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b * 'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*y  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*!% h %Fj ."MFBPCR"\AS.seek 0.524ml F*n' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*R  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*Q% h % ."MFBPCR"\AS.seek 0.53ml F*<' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*E  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*a% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*\\  +107 h  ."MFBPCR"delay 7 seconds +700 F*=% h %< ."MFBPCR"\AS.seek 0.594ml F*:'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*G I +108 h I ."MFBPCR"delay 2 seconds +200 F*J% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*P% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*^  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*u  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*U'{ @CET255601.52 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*S  +42 h  ."MFBPCR"\ASV.dial :PCRlock F*T % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0323h.pcr F*j% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *N'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*V  +22 h ."MFBPCR"\ATV.dial :air3 F*v % +86 h % F*&% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *e'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +21 h F*@% h %Z ."MFBPCR"\AS.seek 0.81ml F*B'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*W% h %> ."MFBPCR"\AS.seek 0.835ml F*p'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*K% h %n ."MFBPCR"\AS.seek 0.82ml F*Y'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*{  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*b  +65 h  F*[% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *d'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*q' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *]'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *;'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *L' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *r'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*~  +32 h  ."MFBPCR"\ATV.dial :air3 F*_ % +87 h % F*D% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *c' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*C% h % ."MFBPCR"\AS.seek 0.81ml F*`'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*) % +22 h % ."MFBPCR"delay 3 seconds +300 F*k% h %> ."MFBPCR"\AS.seek 0.835ml F* 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*}% h %m ."MFBPCR"\AS.seek 0.82ml F*t'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*8  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *i'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b */'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *g'V +5059 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0402h.pcr #Building PCR Slug from elutionWater with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*\  +43 h  ."MFBPCR"\ARV.dial :carrier F*  +11 h  F*'% h %V ."MFBPCR"\AS.seek 0.5ml F*O'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*X n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*z  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*M I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*s% h %Fj ."MFBPCR"\AS.seek 0.524ml F*#' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F* n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*I% h % ."MFBPCR"\AS.seek 0.53ml F*' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*(  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F**% h %< ."MFBPCR"\AS.seek 0.594ml F*,'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*F I +107 h I ."MFBPCR"delay 2 seconds +200 F*$% h %\  ."MFBPCR"\AS.seek 0.6ml F*'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*?  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*.' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*0  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*+  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*9% h % \\ ."MFBPCR"\AS.seek 0.326ml F*6'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*l  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*m % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0402h.pcr F*3% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *"'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*A  +21 h ."MFBPCR"\ATV.dial :air3 F*> % +87 h % F*5% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *2'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*h% h %Z ."MFBPCR"\AS.seek 0.81ml F*7'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*y % +22 h % ."MFBPCR"delay 3 seconds +300 F*!% h %> ."MFBPCR"\AS.seek 0.835ml F* 'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*R  +22 h F*Q% h %n ."MFBPCR"\AS.seek 0.82ml F*n'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*E  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*a  +65 h  F*<% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *\\'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*=' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *G' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *J'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *:' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*P  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*^% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *u' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*S'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*T % +22 h % ."MFBPCR"delay 3 seconds +300 F*j% h %> ."MFBPCR"\AS.seek 0.835ml F*U'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*V  +22 h F*v% h %m ."MFBPCR"\AS.seek 0.82ml F*&'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*N  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *f'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*@' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *e'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *%' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *W'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *B' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *K' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *p'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *{' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *b'V +5060 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0404h.pcr #Building PCR Slug from elutionWater with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*[  +43 h  ."MFBPCR"\ARV.dial :carrier F*Y  +10 h  F*d% h %V ."MFBPCR"\AS.seek 0.5ml F*q'(@ +2548 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *]' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*Z n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *;'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *L'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*~  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*_  +22 h ."MFBPCR"delay 2 seconds +200 F*D% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *r'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*x n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*C% h %Fj ."MFBPCR"\AS.seek 0.524ml F*c' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*) I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*k% h % ."MFBPCR"\AS.seek 0.53ml F*`' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*}% h % ."MFBPCR"\AS.seek 0.536ml F* '< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*8  +107 h  ."MFBPCR"delay 7 seconds +700 F*t% h %< ."MFBPCR"\AS.seek 0.594ml F*i'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F* I +108 h I ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F* '\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +107 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*/' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*w% h % \\ ."MFBPCR"\AS.seek 0.326ml F*\ '{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*' % +21 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0404h.pcr F*g% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *O'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*X  +22 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*z  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*M % +21 h % ."MFBPCR"delay 3 seconds +300 F*s% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +526 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*I% h %n ."MFBPCR"\AS.seek 0.82ml F*#'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*(  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F**' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *F' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *$'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +31 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*0% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *+' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*9  +22 h F*.% h % ."MFBPCR"\AS.seek 0.81ml F*l'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*m % +21 h % ."MFBPCR"delay 3 seconds +300 F*3% h %> ."MFBPCR"\AS.seek 0.835ml F*6'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*A  +21 h F*>% h %m ."MFBPCR"\AS.seek 0.82ml F*5'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*"  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*h' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *2'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *y' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *7' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Q' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *E' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *a'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt270jan0406h.pcr #Building PCR Slug from elutionWater with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*<  +43 h  ."MFBPCR"\ARV.dial :carrier F*n  +10 h  F*\\% h %V ."MFBPCR"\AS.seek 0.5ml F*='(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*G n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *J'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *:'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*P  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*^% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F* n +85 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*% h %Fj ."MFBPCR"\AS.seek 0.524ml F*u' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*T  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*j% h % ."MFBPCR"\AS.seek 0.53ml F*S' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*V  +65 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*v% h % ."MFBPCR"\AS.seek 0.536ml F*U'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*N  +22 h  ."MFBPCR"delay 7 seconds +700 F*&% h %< ."MFBPCR"\AS.seek 0.594ml F*f'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :elutionWater F*@ I +108 h I ."MFBPCR"delay 2 seconds +200 F*e% h %\  ."MFBPCR"\AS.seek 0.6ml F*%'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*W  +108 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*B% h %\  ."MFBPCR"\AS.seek 0.65ml F*o' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*K  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*p  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*{% h % \\ ."MFBPCR"\AS.seek 0.326ml F*['{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*Y  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*d % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt270jan0406h.pcr F*b% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *q'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*Z  +22 h ."MFBPCR"\ATV.dial :air3 F*] % +86 h % F*;% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *~'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*D% h %Z ."MFBPCR"\AS.seek 0.81ml F*L'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*x % +22 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*r'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +22 h F*k% h %n ."MFBPCR"\AS.seek 0.82ml F*c'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*}  +64 h  F*`% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *8'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*t' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*w  +21 h F*/% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*' % +22 h % ."MFBPCR"delay 3 seconds +300 F*g% h %> ."MFBPCR"\AS.seek 0.835ml F*\ 'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*X  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*O  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *z'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *I' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *(' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #t\Asleep after completing 18tun assays .GoodNews.suppressMail "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@08:33:26, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun assays" #\Mission runtime was 4:02.503799 #m\EXIT @1533969390.92CEST @1534505548.08CEST = =ECan =tsimfast =hcore =msimfastScheduler =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi ; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 09:03:12CET04-Jan-70 .delayUntil "09:03:12.00CET04-Jan-70" @CET288192.00 #-> execute "18tun.rb" .GoodNews.suppressMail "Starting 18tun w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:32:36, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 09:03 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNEG\\n/home/esp/esp2/mission/18tun.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:32:53, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 09:03" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:32:57, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC wcr sampling at most 0ml .BACfans.suppressMail "Started mfb in tube 2 at 13:33:02CEST17-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:32:57, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC wcr sampling at most 0ml" F*~% h %&K #tLoading sh1 puck from tube 2 into Collection Clamp #"readyMFB"Readying the MFB C'& #"readyMFB"Homing MFB Syringe h & ."readyMFB"\AFV.select :holdingCoil M&% F* h %\  A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& h & A('X M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast A(6 #tskipping prime and sample collection #\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'%& h &W ."airStroke"\CSV.dial :waste S)'% ."airStroke"\CS.seek 0ml h % E ;3\\0\\0 #t\Calibrating pressure and temperature sensors C'/'4 .t\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +889 C'I% h %4 S)H% h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S)K +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'39 h 9Td +100 C':9 h 9kd +100 C'.9 h 9d +100 C'@9 h 9d +100 C'A9 h 9d +100 C'89 h 9d +100 C'29 h 9d +100 C'D9 h 9d +100 C'E9 h 9 d +100 C'F9 h 9 d +100 C'=9 h 96d +100 C'>9 h 9Ld +100 C')9 h 9bd +100 C'I9 h 9xd +100 C'H9 h 9d +100 C'K9 h 9d +100 C'G9 h 9d +100 C'J9 h 9d +100 C'N9 h 9d +100 C'O9 h 9d +100 C'R9 h 9d +100 C'M9 h 9(d +100 C'S9 h 9>d +100 C'B9 h 9Td +100 C'Q9 h 9id +100 C'L9 h 9d +100 C'P9 h 9d +100 C'V9 h 9d +100 C'W9 h 9d +100 C'T9 h 9d +100 C'Z9 h 9d +100 C'U9 h 9  d +100 C'[9 h 9 d +100 C'Y9 h 9 *d +100 C'\\9 h 9 ?d +100 C'X9 h 9 Td +100 C']9 h 9 j d +100 C'`9 h 9 $d +100 C'_9 h 9 (d +100 C'b9 h 9 ,d +100 C'c9 h 9 0d +100 C'a9 h 9 4d +100 C'f9 h 9 8d +100 C'g9 h 9 % h %Z ."downElevator"\SE.seek :down A()'X +100 (I% h % M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +89 h '\  +82 'X ."downElevator"\SE.coast .t\SC.coast A(5 #tTranferring lysate to PRV .\CSV.dial :air C'G @ + A(6 +17 h @ .t\CS.seek 10ml C'J' +7 h ' .t\CSV.dial between air and CTV C'N ` +19 h ` .t\AIV.dial :bypass C'O { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'R& h & .tHand.open M&M1 +2 h 1 M&S& h & M&B% h %\  .t\Elbow.seek FlushPuck::Garage M&Q(- +166 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.seek :garage M&V'η +91 h 'η .tHand.close M&W/ +150 h / M&T& h &- M&Z% h %η .t\Forearm.seek :retract M&U'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &[(ӷ +264 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &X' +91 h ' .t\CC.seek :closed C'](W +416 h (W C'`& h &W .tHand.open M&_1 +150 h 1 M&b% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%a +151 h P%f% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%g' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%e  +38 h  .t\PS.seek 0ml P%h' +1597 h ' .t\PSV.dial PSR P%^ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%k' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%j  C'm  h +75 .t\PSV.dial :mixloop P%d +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%p  +113 h .t\PS.seek 0ml P%r' +2610 h ' .t\PSV.dial :air P%;  +37 h  .t\PS.seek 2.5ml P%o' +2610 h ' .t\PSV.dial :mixloop P%C +38 h .t\PS.seek 0ml P%t' +2609 h ' .t\PSV.dial :air P%u  +38 h  .t\PS.seek 2.5ml P%x' +2610 h ' .t\PSV.dial :mixloop P%? +37 h .t\PS.seek 0ml P%n' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&i& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +7 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.seek CC M&v' +90 h ' .tHand.close M&~/ +150 h / .t\CC.seek :open C'|( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C' & h & M&\ % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (w& h &  M&z& h &- M&}% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 19.71 Pucks below Top Plate A(' +66 h ' A(& h &  M&#& h & .t\Elbow.seek 2 M&( +7 h ( M&& h & A((& h &  .t\Forearm.seek 2 + 300 counts M&'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A(s'X h & M&,% h %; .t\Forearm.seek :retract M&'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&%(ӷ +142 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &$' +91 h ' .t\CC.seek :closed C'4(W +416 h (W .tHand.open M&1 +150 h 1 M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast A(6 .t\CTV.dial CSV,avoiding: :intake C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'0' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'9'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%3' +2609 h ' .t\PSV.dial :mixloop P%: +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%/  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%l +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%A  +151 h  .t\PS.seek :empty P%8' +2610 h ' .t\CBV.dial :waste1 C'2 @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'E  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'F @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'>  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'6 ` +19 h ` .t\CSV.dial :air C'< @ +38 h @ .t\CS.seek 10ml C'I' +2647 h ' .t\CSV.dial CTV C'H  +38 h  .t\CS.seek 0ml C'.'4 +2647 h '4 .t\CBV.dial :waste1 C'D @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'G  +150 h C'!& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&N% h %\  A(O% h %X A(R. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (M( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (S& h & M&B& h &ӷ M&=% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &L( +143 h ( M&P& h & A()& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &1' +89 h ' A(& h & .t\SE.seek -0.29 Pucks below Top Plate A(@'\\ +265 h '\\ M&W& h & A(T& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &Z'Y +25 h 'Y .t\Elbow.hold M&K, +7 h , .t\Elbow.reconfigure TightElbow A(V- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(Y' +26 h ' M&\\% h %Y A(Q& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&U'υ +21 h 'υ M&&& h & A(`& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &_' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(b' +36 h ' M&a& h & M&f% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +89 h '\  .t\Elbow.seek CC M&e(ӷ +143 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&j/ +150 h / .t\CC.seek :open C'm( +416 h ( A(d& h & A(p& h & M&k& h &ӷ M&;% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &C( +142 h ( M&o& h & A(u& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &t'Y +93 h 'Y .t\Elbow.hold M&?, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &x1 +150 h 1 M&i% h %Y A(q& h & .t\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation .t\Forearm.seek 1 + 300 counts +2 M&{'υ +21 h 'υ M&~& h & A([& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &c' +22 h ' A(]% h % ."downElevator"\SE.seek :down A('X +100 (% h %S M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast .t\SC.coast A(5 .t\PSV.dial :air P%  +2 A(6 +111 h  P%y% h % .t\PS.seek 0.499ml P% ' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'\  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%  +19 h  P%w% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %z' +250 h ' .t\PRV.dial :waste2 P%} @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&('η +91 h 'η .tHand.close M& / +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +90 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&s1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'|& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%5  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%$' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%J  +38 h  .t\PS.seek 0ml P%7' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%0 +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P%n% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %:'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%/  +75 h .tdelay 2 seconds +200 P%l% h %r .t\PS.seek 0.475ml P%A' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%3  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%2'N +4990 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%E  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%F  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %>' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%6 +76 h .t\PTV.dial :PRV,avoiding: :puck P%< @ +75 h @ .t\PS.seek 0ml P%9' +2610 h ' .t\PSV.dial :air P%H  +75 h  .t\PS.seek 2.5ml P%I' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%D +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%G' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%!  +75 h  .t\PS.seek 2.5ml P%N' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%O +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%R  +76 h P%S% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %B' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'= & +50 h & .t\AWV.select :waste C' +6 P%P% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %)'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %%' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%W' +2609 h ' .t\PSV.dial :PML P%T +38 h .t\PS.seek 0ml P%V' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '\\& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'Q& h &W .t\AWV.select :air ."MFBPCR"\ASV.dial :SPEdelivery F*& @@T .t\AIV.dial :bypass F*`  .t\PSV.dial PSR P%a +22 h  ."MFBPCR"delay 20 seconds +91 h P%f% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*L I +129 h I F*@% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *Z'6 +66 .t\PSV.dial :waste1 P%g  +37 h  .t\PS.seek 0ml P%^' +1597 h ' .t\PSV.dial PSR P%U +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Y' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%K' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*j  +22 h ' .t\PSV.dial PSR P%X +21 h  F*4% h %6 ."MFBPCR"\AS.seek 0.329ml F*d'ͯ +92 h P%p% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%k' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*; % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *o'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%u  +38 h  .t\PS.seek 0ml P%h' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*e  +22 h  F*x% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *i' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%q +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%~' +217 ."MFBPCR"\ATV.dial :AIV F*[ I +43 h I F*]% h % ."MFBPCR"\AS.seek 0.469ml F*r' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%C +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P%v +113 h P%t% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%{' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%b  +37 h  .t\PS.seek 0ml P%' +740 h ' ."MFBPCR"delay 5 seconds +500 F*y9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*c n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*M'% +336 h ' .t\PSV.dial PSR P%8 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial PSR P%\ +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%w  +38 h  .t\PS.seek 0ml P% ' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%} +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%z' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %#' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *?'V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%(  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1327 h 'V ."MFBPCR"\ATV.dial :air2 F*'  +42 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *,'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*m I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*s' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %|' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F**'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*5  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*$% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *J'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'0% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'n' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'7  '/  +38 h  +113  .t\CBV.dial :waste1 C'l @ +37 h @ .t\CS.seek 0ml C':'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*3  +21 h ."MFBPCR"delay 3 seconds +300 F*A% h %_ ."MFBPCR"\AS.seek 0.434ml F*E'ݏ +110 h '4 .t\CSV.dial CSR C'F  +38 h  C'2% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'6'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*<  +22 h F*>% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*H'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*9 n +108 h n F*D% h %& ."MFBPCR"\AS.seek 0.092ml F*I'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'!' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'O'4 +2647 h '4 C'R& h &W #tGaraging FlushPuck in Collection Clamp M&S& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +6 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&B' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C')( +416 h ( M&"& h &ӷ M&%% h % .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &W(- +265 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +90 h 'η .tHand.open M&a1 +150 h 1 M&f% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #"WCR"Whole Cell Archive sampling 0ml #Loading wcr puck from tube 2 into Collection Clamp C'L& h & M&@% h %\  A(g% h %X A( . h . ."WCR"\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (1( +201 h (  ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (U& h &  M&^& h &- M&_% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &Y( +166 h ( M&j& h & A(4& h &  ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &X' +89 h ' A(p& h &  ."WCR"\SE.seek 18.71 Pucks below Top Plate A(Z' +77 h ' A(K& h &  M&;& h & ."WCR"\Elbow.seek 2 M&d( +6 h ( M&u& h & A(e& h &  ."WCR"\Forearm.seek 2 + 300 counts M&x'; +23 h '; ."WCR"\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &k/ +150 h / ."downElevator"\SE.seek :down M&o& A(q'X h & M&[% h %; ."WCR"\Forearm.seek :retract M&]'\ +27 h '&0 ."MFBPCR"delay 3 seconds +50 h 'X ."downElevator"\SE.coast A(5 +14 h '\  ."WCR"\Elbow.seek CC M&h(ӷ +142 h (ӷ M&i& h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &ӷ ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &~' +91 h ' ."WCR"\CC.seek :closed C'v(W +3 ."MFBPCR"\ATV.dial :waste2 F*t % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +370 h (W ."WCR"Hand.open M&b1 +150 h 1 M&{% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &y'\ +40 h 'R ."MFBPCR"\ATV.dial :air2 F*c  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *' +29 h '\  ."WCR"\SC.coast #collectSample 0ml w/bubble point of 55psi A(6 #"WCR"\Priming 7ml .Gate.power :sampler,:ON .Intake.select :close .\Exhaust.select :close .\SS.hold S)r+ +6 h + S)8% h % C'& h &W E ;3\\0\\0 #"WCR"\Calibrating pressure sensors .\CTV.dial :air C'  +151 h ."WCR"\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X': +25 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'E  '9  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b 'D'4 +1919 h '4 ."WCR"delay 2:00 +2351 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*H'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F*6 % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *G'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*!  +86 h F*R% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *S'N +440 ."WCR"\CBV.dial :waste2b C'=  +18 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'P' +41 h ' ."WCR"delay 1:00 +528 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +43 h I F*% h %N ."MFBPCR"\AS.seek 0.109ml F*'{ +1229 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F* n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*"  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +6 *%% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *B' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +79 ."WCR"\CTV.dial :air,avoiding: :intake C'\\  +151 h #"WCR"\Fix #2 of 2 (with rnal) for 2:00 .\CSV.dial CSR C'Q  +38 h  C'N% h % #"WCR"\Pulling 2ml of CSR.rnal .\CSR.select :rnal C' +6 ."WCR"\CS.seek 2ml C'a' +26 ."MFBPCR"\AFV.select :holdingCoil F* +7 *f% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *T' +573 h ' ."WCR"delay 5 seconds +500 .\CSR.select :air C' +7 ."WCR"\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'L' +816 h ' #"WCR"\Pumping wcr puck from tube 2 up to 55psi... .\CBV.dial SSV C'@ @ +75 h @ +50 S)g% h % ."WCR"\CBV.dial between CSV and waste2b C'  +94 h  C'U% h % ."WCR"\CSV.dial between air and CTV C'^ ` +57 h ` ."WCR"\CS.seek 7.288ml C'_' +481 h ' ."connect"\CTV.dial CSV,avoiding: :air ."WCR"\CSV.dial CTV C'`  'j  +19 h  +132  ."WCR"\CS.reconfigure CSpressurize .\CS.seek 0ml C'# d@<<b '4'4 +1919 h '4 ."WCR"delay 2:00 +1030 h ' ."MFBPCR"delay 5 seconds +500 F*p% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *W' +3537 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*K% h % ."MFBPCR"\AS.seek 0.279ml F*;'Q +933 ."WCR"\CBV.dial :waste2b C'u  +19 h  ."WCR"\CS.reconfigure CSconfig .\CS.seek :empty C'#P d@b 'e' +41 h ' ."WCR"delay 1:00 +6000 .\CTV.dial :air,avoiding: :intake C'V  +150 h C'k& h &W #"WCR"\Storing wcr puck in Collection Clamp to tube 1 M&o% h %\  A([% h %X A(Y. h . ."WCR"\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (X( +201 h ( ."WCR"\SC.reconfigure SCloose A($.(\ 1~b (i& h & M&C& h &ӷ M&x% h %\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &Z( +142 h ( M&t& h & A(q& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &d' +89 h ' A(]& h & ."WCR"\SE.seek 0.71 Pucks below Top Plate A()'K +254 h 'K M&I& h & A(b& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b ."WCR"\Forearm.seek 1 M&#Q@2^d^2b &{'Y +25 h 'Y ."WCR"\Elbow.hold M&c, +7 h , ."WCR"\Elbow.reconfigure TightElbow A(8- h -+K M&$&.,(\ #b ."WCR"\SE.seek 1.04 Pucks below Top Plate A(' +27 h ' M&% h %Y A(~& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts + M&'υ +21 h 'υ M&1& h & A(r& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' ."WCR"\SE.seek 2.04 Pucks below Top Plate A(\ ' +36 h ' M& & h & M&w% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +90 h '\  ."WCR"\Elbow.seek CC M&(ӷ +142 h (ӷ M& & h &ӷ ."WCR"\Elbow.reconfigure LooseElbow M&$ &.,(#b &M& h &ӷ ."WCR"\Forearm.seek CC M&' +91 h ' ."WCR"Hand.close M&/ +150 h / ."WCR"\CC.seek :open C'( +416 h ( A(}& h & A(& h & M&& h &ӷ M& % h % ."WCR"\Forearm.seek :retract M&#'\ +91 h '\  ."WCR"\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &( +142 h ( M&& h & A(& h & ."WCR"\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &''Y +93 h 'Y ."WCR"\Elbow.hold M&, +6 h , ."WCR"\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &1 +150 h 1 M&?% h %Y A(O& h & ."WCR"\Forearm.force -20 M&5 !o:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation ."WCR"\Forearm.seek 1 + 300 counts +2 M&'υ +21 h 'υ M&& h & A(,& h & ."WCR"\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &' +22 h ' A(% h % ."downElevator"\SE.seek :down A(m'X +100 (% h %B M&5% h % ."WCR"\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +52 'X ."downElevator"\SE.coast A(5 ."WCR"\SC.coast #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'$& h & .tHand.open M&s1 + A(6 + h 1 M&z& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&*(- +166 h (- M&.& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b & & h &- .t\Forearm.seek :garage M&7'η +91 h 'η .tHand.close M&0/ +150 h / M&/& h &- M&n% h %η .t\Forearm.seek :retract M&3'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &A(ӷ +264 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &2& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &l' +91 h ' .t\CC.seek :closed C':(W +416 h (W C'<& h &W .tHand.open M&91 +150 h 1 M&E% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &+'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'J& h &W C'>% h % .t\CSV.dial CSR C'6  +37 h  C'H% h % #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.seek 5ml C'!'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'R% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'G'[ +463 h '[ .t\CSV.dial :waste C'= +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'D'4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'& h &W C'% h %4 .t\CSV.dial :flush C'S  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +385 h 'Q ."MFBPCR"delay 30 seconds +265 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'" @ +38 h @ C'%% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'\\  'Q  +38 h  +113  .t\CBV.dial :waste1 C'N @ +75 h @ .t\CS.seek 0ml C'f'4 +766 h '4 #t\Flushing lower manifold's waste2b port C'P& h &W C'B% h %4 .t\CSV.dial :flush C'a  +75 h  .t\CS.seek 5ml C'@'p +650 h 'p .tdelay 5 seconds +64 ."MFBPCR"\ATV.dial :waste,via: :air3 F*g n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b * 'R +286 .t\CSV.dial :air C'U @ +38 h @ C'^% h %p .t\CS.seek 7ml C'L'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'j  +38 h  .t\CBV.dial :waste2b C'`  +75 h  .t\CS.seek 0ml C'_'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'p  +16 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*T % +21 h  C'K% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +7 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'W'P +15 h % F*u% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *4'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*V I +21 h I F*k% h %v ."MFBPCR"\AS.seek 0.059ml F*o'H +455 h 'P .tdelay 5 seconds +171 h 'H ."MFBPCR"\ATV.dial :air3 F*[ % +21 h % F*Y% h %H ."MFBPCR"\AS.seek 0.069ml F*i' +222 h ' ."MFBPCR"delay 2 seconds +86 .t\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'C' +108 ."MFBPCR"\ATV.dial :bleach F*x  +22 h F*t% h % ."MFBPCR"\AS.seek 0.769ml F*q' q +580 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C']  +37 h  .t\CBV.dial :waste1 C'h @ +76 h @ .t\CS.seek 0ml C'y'4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'I  +38 h  C'b% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b '8'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'~  +38 h  .t\CBV.dial :waste2b C'1  +75 h  .t\CS.seek 0ml C'r'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C'w% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b 'd'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'Z' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C') '{  +38 h +75 .t\CTV.dial :air C'  +151 h .t\CS.seek 0ml C'M'4 +909 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'c& h &W C'% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'v'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'} @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C' +112 h .t\CTV.dial :air .\CS.seek 0ml C''4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +38 h .t\CS.seek 0ml C''4 +32 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +152 h '4 C'?& h &W C'O% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 3ml C','0 +110 F*% h % q ."MFBPCR"\AS.seek 0.794ml F*#' k +396 h '0 .tdelay 5 seconds +129 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h % k ."MFBPCR"\AS.seek 0.779ml F*5'  +49 .t\CSV.dial :air C'$ @ +38 h @ C's% h %0 .t\CS.seek 5ml C'z'p +237 h '  ."MFBPCR"delay 3 seconds +179 h 'p .t\CSV.dial :air .\CS.seek 0ml C''4 +121 ."MFBPCR"\ATV.dial :AIV F*' I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *' +421 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'\ '0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C'.'4 +507 h '4 #t\Flushing lower manifold's waste1 port C' & h &W C'm% h %4 .t\CSV.dial :flush C'X  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'0 @ +37 h @ C'/% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '7  +38 h  +113  .t\CBV.dial :waste1 C'F @ +37 h @ .t\CS.seek 0ml C'2'4 +377 h ' ."MFBPCR"\ATV.dial :waste F** n +128 h n ."MFBPCR"\AS.seek :empty F*3'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*<% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *9' +102 h '4 #t\Flushing lower manifold's waste2b port C'E& h &W C'J% h %4 .t\CSV.dial :flush C'>  +76 h  .t\CS.seek 5ml C'6'p +44 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*H  +22 h F*l% h % ."MFBPCR"\AS.seek 0.579ml F*e' +384 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +38 h @ C'!% h %p .t\CS.seek 7ml C'='i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  +37 h  .t\CBV.dial :waste2b C'  +76 h  .t\CS.seek 0ml C''4 +765 h '4 #tGaraging FlushPuck in Collection Clamp M&S& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &D(ӷ +7 h (ӷ M&"& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &%& h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&\\/ +150 h / .t\CC.seek :open C'Q( +416 h ( M&N& h &ӷ M&% h % .t\Forearm.seek :retract M&P'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &B(- +264 h (- M&a& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &f& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &g'η +91 h 'η .tHand.open M&;1 +150 h 1 M&U% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &^'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'@& h & .tHand.open M&j1 +2 h 1 M&`& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &L(- +7 h (- M&p& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &K& h &- .t\Forearm.seek :garage M&T'η +91 h 'η .tHand.close M&u/ +150 h / M&_& h &- M& % h %η .t\Forearm.seek :retract M&V'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &k(ӷ +264 h (ӷ M&4& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &[& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &Y' +91 h ' .t\CC.seek :closed C'o(W +416 h (W C'x& h &W .tHand.open M&t1 +150 h 1 M&W% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &i'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C']& h &W C'h% h %4 .t\CSV.dial CSR C'C  +37 h  C'I% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'b'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'y% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '8'[ +463 h '[ .t\CSV.dial :waste C'~ +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '1'4 +2009 h '4 #t\Purging external Intake C'& h &W C' % h %4 .t\CSV.dial :flush C'w  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'r' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'd '{  +37 h +38 .t\CTV.dial :intake C')  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C' '4 +415 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*Z % +22 h % ."MFBPCR"delay 3 seconds +172 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'M  +75 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'c  +46 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*'\ 48 +29 h  .t\CS.seek 5ml C''p +496 h '\ 48 ."MFBPCR"delay 3 seconds +155 h 'p .tdelay 5 seconds +145 ."MFBPCR"\ATV.dial :bleach F*}  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*v' +324 h '  ."MFBPCR"delay 3 seconds +9 .t\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +254 ."MFBPCR"\ATV.dial :ASV F* n +85 h n ."MFBPCR"\ASV.dial :wasteR F*  +43 h  ."MFBPCR"\AS.seek 0ml F*'V +34 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'? +113 h .t\CTV.dial :air .\CS.seek 0ml C'O'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +37 h .t\CS.seek 0ml C''4 +507 h '4 C'q& h &W C'% h %4 .t\CSV.dial :flush C'  +37 h  .t\CS.seek 3ml C'$'0 +507 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C's @ +38 h @ C'#% h %0 .t\CS.seek 5ml C','p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'''4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'z'0 +506 h '0 .t\CSV.dial :waste C'5 +76 h .t\CS.seek 0ml C''4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &\ (ӷ +7 h (ӷ M& & h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &m& h &ӷ .t\Forearm.seek CC M&X' +91 h ' .tHand.close M&./ +150 h / .t\CC.seek :open C'0( +416 h ( M&/& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &7(- +264 h (- M&F& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &n& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &*'η +91 h 'η .tHand.open M&1 +150 h 1 M&<% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &E'\ +91 h '\  #t\Completed [coreNEG] begun 09:03:43 #\Extending 18tun until completion of MFBPCR +3920 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *J' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F*> % +107 h % F*3% h %V ."MFBPCR"\AS.seek 0.59ml F*H' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*l n +107 h n ."MFBPCR"\ASV.dial :wasteR F*2% h %  ."MFBPCR"\AS.seek 0.09ml F*9' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*R  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*! I +86 h I F*6% h % ."MFBPCR"\AS.seek 0.12ml F*G' +625 h ' ."MFBPCR"\ATV.dial :air3 F* % +22 h % F*=% h % ."MFBPCR"\AS.seek 0.13ml F*S' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*"  +22 h F*%% h % ."MFBPCR"\AS.seek 0.23ml F*\\' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*A % +21 h % ."MFBPCR"delay 3 seconds +300 F*+% h % ."MFBPCR"\AS.seek 0.255ml F*:'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +21 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*a n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*f  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *D'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *;% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *U'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F*P @F@ ."MFBPCR"delay 3:00 h . +18000 ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F*j I +86 h I ."MFBPCR"\AS.seek 0ml F*`'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*p'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*K  +43 h  #"MFBPCR"\Swish #1 of 2 F*g% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *u'\  +1031 h '\  F*_% h %\  ."MFBPCR"\AS.seek 0.5ml F* '(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*B% h %(A ."MFBPCR"\AS.seek 0.6ml F*^'\  +1032 h '\  F*T% h %\  ."MFBPCR"\AS.seek 0.5ml F*4'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*[% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *L' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*V % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *Q'V +3472 h 'V ."MFBPCR"\SPE.coast F*x  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0410h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*t  +65 h  ."MFBPCR"\ARV.dial :carrier F*W  +10 h  F*]% h %V ."MFBPCR"\AS.seek 0.5ml F*h'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *C' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*I n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *Y'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*y  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*b  +22 h ."MFBPCR"delay 2 seconds +200 F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *8'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F* % h %Fj ."MFBPCR"\AS.seek 0.524ml F*w' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*1  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*{% h % ."MFBPCR"\AS.seek 0.53ml F*d' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*) % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*r% h % ."MFBPCR"\AS.seek 0.536ml F*Z'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*M  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*c% h %\  ."MFBPCR"\AS.seek 0.6ml F*e'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*}% h %\  ."MFBPCR"\AS.seek 0.65ml F*' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*  +43 h  ."MFBPCR"\ASV.dial :waste1 F*  +64 h ."MFBPCR"\ARV.dial :air1 F*  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*? % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refi ; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0410h.pcr F*v% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*O  +21 h ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *q'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +22 h % ."MFBPCR"delay 3 seconds +300 F*#% h %> ."MFBPCR"\AS.seek 0.835ml F*$'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +22 h F*'% h %n ."MFBPCR"\AS.seek 0.82ml F*5'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*z  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *m'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*.' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *k'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *o'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b */' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*X  +32 h  ."MFBPCR"\ATV.dial :air3 F*F % +86 h % F*n% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *\ ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*<% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F** % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*J'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*>  +22 h F*3% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*l  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *2'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*H' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *R'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *!' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *6'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *9' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *=' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *G'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *"' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *%'V +5060 h 'V #"MFBPCR"\Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0412h.pcr #Building PCR Slug from ASV with MM1 mix, PP1 primer #\Priming with carrier .\ATV.dial ARV F*S  +43 h  ."MFBPCR"\ARV.dial :carrier F*A  +10 h  F*+% h %V ."MFBPCR"\AS.seek 0.5ml F*\\'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *N' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *:'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *a'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*f  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*;% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *D'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F*U I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*j% h %Fj ."MFBPCR"\AS.seek 0.524ml F*@' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP1 F*` % +22 h % #"MFBPCR"\Adding PP1 .delay 2 seconds +200 F*K% h % ."MFBPCR"\AS.seek 0.53ml F*g' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*p  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*_% h % ."MFBPCR"\AS.seek 0.536ml F*u'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*B  +21 h  ."MFBPCR"delay 7 seconds +700 F* % h %< ."MFBPCR"\AS.seek 0.594ml F*T'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*^ n +21 h n ."MFBPCR"delay 2 seconds +200 F*[% h %\  ."MFBPCR"\AS.seek 0.6ml F*4'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*V  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*L% h %\  ."MFBPCR"\AS.seek 0.65ml F*x' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*t  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*W  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*]% h % \\ ."MFBPCR"\AS.seek 0.326ml F*Q'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*h  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*I % +22 h % ."MFBPCR"delay 5 seconds +500 #Running IPc ProfileIPc thermocycling ."PCR"\PCR.program ProfileIPc #\ProfileIPc should complete in < 1:50:49 .\PCR.run 2:23:31.25 +664900 #"MFBPCR"\PCR for IPc completed -- closed /var/log/esp/IPc70jan0412h.pcr F*C% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *Y'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*y  +22 h ."MFBPCR"\ATV.dial :air3 F*b % +86 h % F*~% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F* % h %Z ."MFBPCR"\AS.seek 0.81ml F*8'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*1 % +22 h % ."MFBPCR"delay 3 seconds +300 F*{% h %> ."MFBPCR"\AS.seek 0.835ml F*w'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*)  +22 h F*r% h %n ."MFBPCR"\AS.seek 0.82ml F*d'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*M  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +65 h  F*Z% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*c' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *}'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *e' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F* % +87 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*?% h % ."MFBPCR"\AS.seek 0.81ml F*&'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*v % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*O'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*q' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *#' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *,' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *''V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *z'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b * 'V +5059 h 'V #"MFBPCR"\Setting up PCR for Rp -- writing /var/log/esp/Rp70jan0415h.pcr #Building PCR Slug from ASV with MM2 mix, PP3 primer #\Priming with carrier .\ATV.dial ARV F*5  +43 h  ."MFBPCR"\ARV.dial :carrier F*m  +11 h  F*.% h %V ."MFBPCR"\AS.seek 0.5ml F*k'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*o n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b */'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *X'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*F  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*n  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*<  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*\ % h %Fj ."MFBPCR"\AS.seek 0.524ml F**' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP3 F*  +21 h #"MFBPCR"\Adding PP3 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*>' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*3  +22 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*J% h % ."MFBPCR"\AS.seek 0.536ml F*l'< +620 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +108 h  ."MFBPCR"delay 7 seconds +700 F*2% h %< ."MFBPCR"\AS.seek 0.594ml F*H'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*R n +21 h n ."MFBPCR"delay 2 seconds +200 F*!% h %\  ."MFBPCR"\AS.seek 0.6ml F*6'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*9  +22 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.65ml F*=' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*G  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*"  +21 h #"MFBPCR"\Positioning Slug by 0.324ml F*S% h % \\ ."MFBPCR"\AS.seek 0.326ml F*A'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*+  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*% % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Rp Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Rp completed -- closed /var/log/esp/Rp70jan0415h.pcr F*\\% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +1025 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*N  +21 h ."MFBPCR"\ATV.dial :air3 F*: % +87 h % F*f% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*;  +22 h F*a% h %Z ."MFBPCR"\AS.seek 0.81ml F*U'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*j % +22 h % ."MFBPCR"delay 3 seconds +300 F*D% h %> ."MFBPCR"\AS.seek 0.835ml F*`'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*K  +22 h F*@% h %n ."MFBPCR"\AS.seek 0.82ml F*p'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*_  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*g  +65 h  F*B% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b * 'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*u' +3070 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *^'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *[' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *T'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *V' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *L'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*4  +32 h  ."MFBPCR"\ATV.dial :air3 F*t % +86 h % F*W% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *]' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*x  +22 h F*h% h % ."MFBPCR"\AS.seek 0.81ml F*I'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*P % +21 h % ."MFBPCR"delay 3 seconds +300 F*C% h %> ."MFBPCR"\AS.seek 0.835ml F*Q'm +526 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*y  +22 h F*b% h %m ."MFBPCR"\AS.seek 0.82ml F*~'q +323 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*Y  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F* ' +5776 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *1' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *{'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *8' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *)'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *r' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *w'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *M' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +5060 h 'V #"MFBPCR"\Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0417h.pcr #Building PCR Slug from ASV with MM1 mix, PP2 primer #\Priming with carrier .\ATV.dial ARV F*Z  +43 h  ."MFBPCR"\ARV.dial :carrier F*d  +10 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*c'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *}'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *e'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*  +22 h ."MFBPCR"delay 2 seconds +200 F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM1 F* I +43 h I ."MFBPCR"delay 2 seconds +200 #\Adding MM1 F*?% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1826 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP2 F*v n +22 h n #"MFBPCR"\Adding PP2 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*&' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air2 F*  +21 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.536ml F*O'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +107 h  ."MFBPCR"delay 7 seconds +700 F*% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*q n +21 h n ."MFBPCR"delay 2 seconds +200 F*s% h %\  ."MFBPCR"\AS.seek 0.6ml F*#'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*,% h %\  ."MFBPCR"\AS.seek 0.65ml F*'' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*$  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*z  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*% h % \\ ."MFBPCR"\AS.seek 0.326ml F*5'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*m  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*. % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Ss Profile60C350 thermocycling ."PCR"\PCR.program Profile60C350 #\Profile60C350 should complete in < 1:48:05 .\PCR.run 2:20:06.25 +648500 #"MFBPCR"\PCR for Ss completed -- closed /var/log/esp/Ss70jan0417h.pcr F* % h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *k'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*o  +22 h ."MFBPCR"\ATV.dial :air3 F*i % +86 h % F*/% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *F'Z +223 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*n  +21 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*X'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*< % +22 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F**'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*3  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*J  +65 h  F*>% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2036 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*2' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *l'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *R' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *!'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *H' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *9'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*6 % +87 h % F*G% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *"' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*S  +22 h F*=% h % ."MFBPCR"\AS.seek 0.81ml F*+'> +16109 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*% % +22 h % ."MFBPCR"delay 3 seconds +300 F*\\% h %> ."MFBPCR"\AS.seek 0.835ml F*A'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*N  +22 h F*:% h %m ."MFBPCR"\AS.seek 0.82ml F*f'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*  +22 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *;'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*a' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *j' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *D'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *U' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *K'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *@' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *`'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *_' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *g'V +5059 h 'V #"MFBPCR"\Setting up PCR for Bb -- writing /var/log/esp/Bb70jan0419h.pcr #Building PCR Slug from ASV with MM3 mix, PP5 primer #\Priming with carrier .\ATV.dial ARV F*B  +43 h  ."MFBPCR"\ARV.dial :carrier F*p  +11 h  F* % h %V ."MFBPCR"\AS.seek 0.5ml F*u'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *^' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*[ n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *T'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *V'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*4  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*t  +22 h ."MFBPCR"delay 2 seconds +200 F*W% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *L'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*x n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*h% h %Fj ."MFBPCR"\AS.seek 0.524ml F*]' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP5 F*P I +22 h I #"MFBPCR"\Adding PP5 .delay 2 seconds +200 F*C% h % ."MFBPCR"\AS.seek 0.53ml F*I' +620 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F*y % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*b% h % ."MFBPCR"\AS.seek 0.536ml F*Q'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*Y  +107 h  ."MFBPCR"delay 7 seconds +700 F*~% h %< ."MFBPCR"\AS.seek 0.594ml F*'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F* n +21 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*1'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*{  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*8% h %\  ."MFBPCR"\AS.seek 0.65ml F*)' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*r  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*w  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*M% h % \\ ."MFBPCR"\AS.seek 0.326ml F*Z'{ +32595 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*d  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Bb Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 #"MFBPCR"\PCR for Bb completed -- closed /var/log/esp/Bb70jan0419h.pcr F*% h %{ #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *c'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +86 h % F*}% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b * 'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h %Z ."MFBPCR"\AS.seek 0.81ml F*e'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*?% h %> ."MFBPCR"\AS.seek 0.835ml F*'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*v  +22 h F*% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*&% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *O'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *q' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*,  +32 h  ."MFBPCR"\ATV.dial :air3 F*# % +86 h % F*$% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *z' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*'% h % ."MFBPCR"\AS.seek 0.81ml F*m'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*. % +22 h % ."MFBPCR"delay 3 seconds +300 F* % h %> ."MFBPCR"\AS.seek 0.835ml F*5'm +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*o  +22 h F*i% h %m ."MFBPCR"\AS.seek 0.82ml F*/'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*k  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *n'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *F'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *<' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *\ 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *X' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *3' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *J'V +5060 h 'V #"MFBPCR"\Setting up PCR for Tt2 -- writing /var/log/esp/Tt270jan0422h.pcr #Building PCR Slug from ASV with MM3 mix, PP6 primer #\Priming with carrier .\ATV.dial ARV F*>  +43 h  ."MFBPCR"\ARV.dial :carrier F**  +11 h  F*% h %V ."MFBPCR"\AS.seek 0.5ml F*2'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *l' +2899 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*R n +130 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *!'R +5064 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *H'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 #"MFBPCR"Building Slug .\ATV.dial :ARV F*  +130 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*6  +21 h ."MFBPCR"delay 2 seconds +200 F*G% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *9'Fj +621 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM3 F*S n +86 h n ."MFBPCR"delay 2 seconds +200 #\Adding MM3 F*=% h %Fj ."MFBPCR"\AS.seek 0.524ml F*"' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP6 F*%  +21 h  #"MFBPCR"\Adding PP6 .delay 2 seconds +200 F*\\% h % ."MFBPCR"\AS.seek 0.53ml F*+' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air1 F*N  +64 h #"MFBPCR"adding ARV air .delay 2 seconds +200 F*:% h % ."MFBPCR"\AS.seek 0.536ml F*A'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*  +22 h  ."MFBPCR"delay 7 seconds +700 F*f% h %< ."MFBPCR"\AS.seek 0.594ml F*;'\ +5848 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*a n +22 h n ."MFBPCR"delay 2 seconds +200 F*% h %\  ."MFBPCR"\AS.seek 0.6ml F*j'\  +620 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*D  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*U% h %\  ."MFBPCR"\AS.seek 0.65ml F*K' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*@  +43 h  ."MFBPCR"\ASV.dial :waste1 .\ARV.dial :air1 F*`  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*_% h % \\ ."MFBPCR"\AS.seek 0.326ml F*B'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*p  +43 h  ."MFBPCR"\ASV.dial :PCRlock F* % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt2 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 @CET342883.85 #"MFBPCR"\PCR for Tt2 completed -- closed /var/log/esp/Tt270jan0422h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*g @@T ."MFBPCR"\ASV.dial :SPEdelivery F*u  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *['(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*^  +151 h F*T% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *4' +423 h ' ."MFBPCR"\ATV.dial :ASV F*t n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*W  +43 h  F*V% h % ."MFBPCR"\AS.seek 0.62ml F*x'\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*h n +150 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *L'R +3154 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *P'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*C'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*] % +43 h % F*y% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *b' +423 h ' ."MFBPCR"\ATV.dial :air3 F*I% h % ."MFBPCR"\AS.seek 0.53ml F*Y' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*~  +21 h F*Q% h % ."MFBPCR"\AS.seek 0.71ml F* ' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % H ."MFBPCR"\AS.seek 0.735ml F*{' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +22 h F*1% h % Ű ."MFBPCR"\AS.seek 0.72ml F*r' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*w n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*M% h % zG ."MFBPCR"\AS.seek 0.365ml F*)' +7161 h ' ."MFBPCR"\ATV.dial :waste F*d n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*Z% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b * ' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*}% h % ."MFBPCR"\AS.seek 0.579ml F*c' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F* '\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +21 h F*?% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*e' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*v n +86 h n ."MFBPCR"\ASV.dial :wasteR F*  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F*& % +108 h % F*% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*O% h % ."MFBPCR"\AS.seek 0.58ml F*q' q +11484 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*s % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h % q ."MFBPCR"\AS.seek 0.605ml F*,'\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*#  +21 h F*$% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F* n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*'  +21 h  ."MFBPCR"\AS.seek 0ml F*z'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *.' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b * 'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*m  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *o' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*i n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *5'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*k n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b */'R +202 h 'R ."MFBPCR"\SPE.coast F*n  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F*F n +150 h n ."MFBPCR"\ASV.dial :SPErecovery F*<  +43 h  F*\ % h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *X' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*3  +43 h  F*>% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b **': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*J% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *2'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*R n +65 h n F*l% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *!' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F* I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *6'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*H  +43 h  F*S% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *=': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*9  +64 h  F*%% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *\\'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*" n +65 h n F*N% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *:' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*+ I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*f  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *A': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*a n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *'Q +1462 h 'Q ."MFBPCR"\ATV.dial :waste F*; n +151 h n ."MFBPCR"\AS.seek 0ml F*D'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*U  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*j % +43 h % F*@% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *`' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*K% h % ."MFBPCR"\AS.seek 0.96ml F*p'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*u% h %0 ."MFBPCR"\AS.seek 0.985ml F*B' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*^  +22 h F*T% h % ."MFBPCR"\AS.seek 0.97ml F*['c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*t I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *W' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *V'R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *4' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *h'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *L'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*P  +43 h  F*]% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *y' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *C' +9988 h ' ."MFBPCR"\ATV.dial AIV F*I I +43 h I ."MFBPCR"\AS.seek 0.02ml F*b' +9880 h ' ."MFBPCR"\ATV.dial :waste F*~ n +129 h n ."MFBPCR"\AS.seek 0ml F*Q'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*Y% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ASV.dial :waste1 F*  +22 h ."MFBPCR"\ATV.dial :air3 F* % +43 h % F*8% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *1'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*w% h %Z ."MFBPCR"\AS.seek 0.81ml F*M'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*r % +22 h % ."MFBPCR"delay 3 seconds +300 F*d% h %> ."MFBPCR"\AS.seek 0.835ml F*)'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*Z% h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*}  +64 h  F* % h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *c'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *?'V +2723 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *v'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*  +32 h  ."MFBPCR"\ATV.dial :air3 F*e % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*&  +21 h F*% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*O% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*s  +22 h F*% h %m ."MFBPCR"\AS.seek 0.82ml F*q'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*#  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *$'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*,' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *'' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *z' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *.'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *m' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *i' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *o'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *k' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*n n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *5'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*/ % +108 h % ."MFBPCR"\AS.seek 0ml F*F'V +2547 h 'V #t\Asleep after completing 18tun assays .GoodNews.suppressMail "{1 => 3, 2 => 19, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@13:33:53, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun assays" #\Mission runtime was 2:35.555218 #-> exit #m\EXIT @1534505738.38CEST @1534775019.44CEST = =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =ECan =msimfastScheduler =tsimfast =hcore @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 01:27:32CET05-Jan-70 .delayUntil "01:27:32.00CET05-Jan-70" @CET347252.00 #-> runSPE(:extSrc) #\Extracting Nucleic Acid from extSrc .\SPE.coast !o: BusOff:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"ESolid Phase Extraction Heater program: core bus was not powered:@checkpoint: omitted #-> ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig S)#.0X% h % M&?% h %\  M&@& h &_ A(A& h &  A(B% h %X C'C9 h 9 P%D9 h 9 #t-> MFB.ready! F*E% h %&K #tReadying the MFB #Homing MFB Syringe .\AFV.select :holdingCoil F* +6 *F% h %&K .t\ATV.dial :waste2 F*G % +129 h % .t\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b *H' +2410 h ' .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 1ml F*I' +5285 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ATV.dial :waste F*J n +108 h n .t\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b *K'R +5064 h 'R .t\ATV.dial :waste .\ARV.dial between carrier and air1 F*L  +12 h  .t\ASV.dial :waste1 F*M  h #t-> runSPE(:extSrc) #\Extracting Nucleic Acid from extSrc .\SPE.hold 2:46:40,55C F*N @@T .t\ASV.dial :SPEdelivery F*O  +21 h  .tdelay 20 seconds +2000 .\AFV.select :holdingCoil .\ATV.dial :air2 F*P  +86 h  F*Q% h %R .t\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *R' +1430 h ' .tdelay 3 seconds +300 .\ATV.dial :extSrc F*S  +108 h  F*T% h % .t\AS.seek 0.469ml F*U' +8066 h ' .tdelay 5 seconds +500 F*V9 h 9 #t\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*W n +43 h n .t\ASV.dial :SPEdelivery F*X% h % .t\AS.seek 0.041ml F*Y'% +8628 h '% .tdelay 10 seconds +1000 #Rinsing external lysate line extSrc .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5535 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\ATV.dial :extSrc F*[  +42 h  .t\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *\\'V +5060 h 'V .t\ATV.dial :air2 F*]  +108 h  F*^% h %V .t\AS.reconfigure ASslow0 .\AS.seek 0.05ml F*#,,'T20 b *_'Q +528 h 'Q .t\ATV.dial :extSrc F*`  +108 h  F*a% h %Q .t\AS.seek 0.01ml F*b' +428 h ' .t\ATV.dial :waste F*c n +108 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *d'R +88 h 'R #tethanol wash .\ATV.dial :air2 F*e  +86 h  F*f% h %R .t\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *g' +1022 h ' .tdelay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*h  +43 h F*i% h % .t\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *j'_ +8066 h '_ .tdelay 3 seconds +300 .\ATV.dial :air1 F*k  +21 h .tdelay 3 seconds +300 F*l% h %_ .t\AS.seek 0.434ml F*m'ݏ +526 h 'ݏ .tdelay 3 seconds +300 .\ATV.dial :ethanol F*n  +22 h F*o% h %ݏ .t\AS.seek 0.419ml F*p'& +323 h '& .tdelay 3 seconds +300 .\ATV.dial ASV F*q n +108 h n F*r% h %& .t\AS.seek 0.092ml F*s'&0 +6597 h '&0 .tdelay 3 seconds +300 .\ATV.dial :waste2 F*t % +43 h % .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +560 h 'R .t\ATV.dial :air2 F*v  +22 h  .t\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *w' +20147 h ' .tdelay 5 seconds +500 .\ATV.dial ASV F*x n +64 h n .t\ASV.dial :SPEdelivery .\AS.seek 0.02ml F*y' +19730 h ' .tdelay 5 seconds +500 .\ATV.dial :air2 F*z  +65 h  F*{% h % .t\AS.seek 0.52ml F*|' +10077 h ' .tdelay 5 seconds +500 .\ATV.dial ASV F*} n +65 h n .t\ASV.dial :wasteR F*~  +21 h  .t\AS.seek 0.3ml F*': +4446 h ': .tdelay 20 seconds +2000 .\AS.seek 0.07ml F*'w +4647 h 'w .tdelay 20 seconds +2000 .\ATV.dial :waste2 F* % +43 h % .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +433 h 'R #t\Elution .\ATV.dial :air1 F*  +86 h F*% h %R .t\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *'N +1027 h 'N .tdelay 3 seconds +300 .\ATV.dial :ElutionWater F* I +43 h I F* % h %N .t\AS.seek 0.109ml F*\ '{ +1228 h '{ .tdelay 3 seconds +300 .\ATV.dial ASV,via: :air2 F* n +173 h n .t\ASV.dial :SPEelution F*  +43 h .t\AFV.select :carrierSolution F* +6 * % h %{ .t\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *' ?x +3300 h ' ?x .tdelay 3 seconds +300 .\AFV.select :holdingCoil F* +7 *% h % ?x .t\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *' +5753 h ' .tdelay 5 seconds +500 F*% h % .t\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *' +3536 h ' .tdelay 1:00 +6000 #pushing eluate to holding coil F*% h % .t\AS.seek 0.279ml F*'Q +16105 h 'Q .tdelay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F* n +151 h n .t\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +1635 h 'R #t\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F* % +43 h % F*% h %R .t\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *'v +625 h 'v .t\ATV.dial :ElutionWater F* I +21 h I F* % h %v .t\AS.seek 0.059ml F*'H +626 h 'H .t\ATV.dial :air3 F*# % +21 h % F*% h %H .t\AS.seek 0.069ml F*' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*)  +22 h F*+% h % .t\AS.seek 0.149ml F*,'D +1630 h 'D .tdelay 3 seconds +300 .\ATV.dial :air3 F*- % +21 h % .tdelay 3 seconds +300 F*% h %D .t\AS.seek 0.174ml F*'n +526 h 'n .tdelay 3 seconds +300 .\ATV.dial :bleach F*&  +21 h F*(% h %n .t\AS.seek 0.159ml F*0'w +324 h 'w .tdelay 3 seconds +300 .\ATV.dial :extSrc F*1  +43 h  .t\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *2' +855 h ' .t\ATV.dial :waste F*3 n +108 h n .t\AS.seek :empty F*4'R +116 h 'R .t\ATV.dial :air3 F*5 % +43 h % F*6% h %R .t\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *7' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*8  +22 h F*9% h % .t\AS.seek 0.579ml F*:' +11484 h '  .tdelay 3 seconds +300 .\ATV.dial :air3 F*; % +22 h % .tdelay 3 seconds +300 F*<% h %  .t\AS.seek 0.604ml F*='\ 48 +525 h '\ 48 .tdelay 3 seconds +300 .\ATV.dial :bleach F*>  +22 h F*?% h %\ 48 .t\AS.seek 0.589ml F*@' +324 h '  .tdelay 3 seconds +300 .\ATV.dial :ASV F*A n +86 h n .t\ASV.dial :wasteR F*B  +43 h  .t\AS.seek 0ml F*C'V +11870 h 'V .tdelay 1:00 +6000 .\AFV.select :carrierSolution F* +7 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +5770 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +7 .tdelay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *E'V +20131 h 'V .tdelay 20 seconds +2000 .\ATV.dial :air3 F*F % +108 h % F*G% h %V .t\AS.seek 0.59ml F*' +11886 h '  .tdelay 3 seconds +300 .\ATV.dial :ASV F*H n +108 h n .t\ASV.dial :wasteR F*J% h %  .t\AS.seek 0.09ml F*I' +10077 h ' .tdelay 30 seconds +3000 .\ATV.dial :extSrc F*L  +43 h  .tdelay 3 seconds +300 .\ATV.dial :ElutionWater F*M I +86 h I F*O% h % .t\AS.seek 0.12ml F*P' +625 h ' .t\ATV.dial :air3 F*Q % +21 h % F*K% h % .t\AS.seek 0.13ml F*S' +222 h ' .tdelay 2 seconds +200 .\ATV.dial :bleach F*T  +22 h F*R% h % .t\AS.seek 0.23ml F*V' +2033 h ' .tdelay 3 seconds +300 .\ATV.dial :air3 F*W % +22 h % .tdelay 3 seconds +300 F*X% h % .t\AS.seek 0.255ml F*U'X +525 h 'X .tdelay 3 seconds +300 .\ATV.dial :bleach F*Y  +22 h F*[% h %X .t\AS.seek 0.24ml F*Z' +324 h '  .tdelay 3 seconds +300 .\ATV.dial :ASV F*] n +86 h n .t\ASV.dial :SPEdelivery F*^  +21 h  .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *\\'(@ +1519 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 *`% h %(@ .t\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *a'5 +6659 h '5 .t\SPE.hold 2:46:40,95C .delay 3:00 F*_ @F@ h * +18000 .t\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +4794 h ' .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .tdelay 1 second +100 .\ASV.dial :wasteD F*e I +86 h I .t\AS.seek 0ml F*f'V +5771 h 'V #t\Priming Eluate .\AFV.select :carrierSolution F* +6 .tdelay 2 seconds +200 .\AS.seek 0.5ml F*b'(@ +2899 h '(@ .tdelay 1 second +100 .\AFV.select :holdingCoil F* +6 .t\ATV.dial ASV .\ASV.dial :SPErecovery F*d  +43 h  #t\Swish #1 of 2 F*h% h %(@ .t\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *i'\  +1032 h '\  F*g% h %\  .t\AS.seek 0.5ml F*k'(A +1031 h '(A #t\Swish #2 of 2 F*l% h %(A .t\AS.seek 0.6ml F*j'\  +1032 h '\  F*n% h %\  .t\AS.seek 0.5ml F*o'(B +1032 h '(B .tdelay 3 seconds +300 F*m% h %(B .t\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *q' M +3722 h ' M .tdelay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*r % +43 h % .t\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *p'V +3472 h 'V .t\SPE.coast F*t  h  .t\ASV.dial :SPErecovery #-> recover 0.08 .\ASV.dial :SPErecovery .\ATV.dial :recoveryTube F*s  +86 h  F*v% h %V .t\AS.reconfigure ASslow1 .\AS.seek 0.2ml F*#,,'Td20 b *u'D +4044 h 'D .t\ATV.dial ASV F*x n +43 h n F*w% h %D .t\AS.seek 0.28ml F*z'8 +1630 h '8 .t\ATV.dial :air2 F*{  +64 h  F*y% h %8 .t\AS.seek 0.29ml F*}'~ +223 h '~ .t\ATV.dial :recoveryTube F*~  +107 h  F*|% h %~ .t\AS.seek 0.02ml F*' +5452 h ' F*% h % #t-> AS.reconfigure ASslow1 AS.seek 0.2ml !o:SyntaxError:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"Pcompile error\ (ESP):6: syntax error, unexpected tIDENTIFIER, expecting $end #-> exit #m\EXIT @1534776431.19CEST @1534840979.39CEST = =tsimfast =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =msimfastScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi !; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 02:24:15CET05-Jan-70 .delayUntil "02:24:15.00CET05-Jan-70" @CET350655.00 !o:SignalException :\ signoi:bt["1/home/esp/esp2/lib/threads.rb:721:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg" SIGHUP:@timestampu: Time ` !o:Errno::EIO:bt["7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475:\ errnoi\ : mesg"Input/output error #m\EXIT @1534841080.34CEST @1534841139.45CEST = =ECan =tsimfast =msimfastScheduler =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi!; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #\Cleanly exited at 02:24:15CET05-Jan-70 .delayUntil "02:24:15.00CET05-Jan-70" @CET350655.00 #-> initialPurge #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage !o:Puck::Warning:bt[ "7/home/esp/esp2/utils/puckmoves.rb:223:in `giveup!'"2/home/esp/esp2/utils/puckmoves.rb:726:in `to'"6/home/esp/esp2/utils/puckmoves.rb:777:in `loadAt'"=/home/esp/esp2/utils/puckmoves.rb:898:in `loadFlushPuck'">/home/esp/esp2/protocol/utilities.rb:75:in `initialPurge'"(ESP):1:in `irb_binding'"A/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52:in `irb_binding'"0/opt/mbari/lib/ruby/1.8/irb/workspace.rb:52: mesg"BRetaining claim on Arm because Hand is likely holding a puck! !o:I2C::NodeOffline:bt["7/opt/mbari/lib/ruby/1.8/irb.rb:177:in `eval_input'":/opt/mbari/lib/ruby/1.8/irb.rb:277:in `signal_status'"7/opt/mbari/lib/ruby/1.8/irb.rb:163:in `eval_input'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:241:in `each_top_level_statement'":/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `loop'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:227:in `each_top_level_statement'";/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `catch'"N/opt/mbari/lib/ruby/1.8/irb/ruby-lex.rb:226:in `each_top_level_statement'"7/opt/mbari/lib/ruby/1.8/irb.rb:162:in `eval_input'"1/opt/mbari/lib/ruby/1.8/irb.rb:69:in `start'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `catch'"1/opt/mbari/lib/ruby/1.8/irb.rb:67:in `start'"-/home/esp/esp2/lib/esp.rb:200:in `shell'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"Wcollection microcontroller never signed on while reading Collection Clamp's status:@checkpoint: omitted #-> ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ C' 2\ F*\ 9\ *\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X% h %\  M&?& h &_ A(@& h &  A(A% h %X C'B9 h 9 P%C9 h 9 #t-> initialPurge #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'D& h & .tHand.open M&E1 +150 h 1 M&F& h &_ M&G% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &H(- +166 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &K'η +91 h 'η .tHand.close M&L/ +150 h / M&M& h &- M&N% h %η .t\Forearm.seek :retract M&O'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &P(ӷ +264 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &S' +91 h ' .t\CC.seek :closed C'T(W +416 h (W C'U& h &W .tHand.open M&V1 +150 h 1 M&W% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'Y& h &W C'Z% h %4 .t\CSV.dial CSR C'[  +151 h  C'\\% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ']'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '^% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '_'[ +462 h '[ .t\CSV.dial :waste C'` +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'a'4 +2010 h '4 #t\Purging external Intake C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'e' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'f 'g  h +75 .t\CTV.dial :intake C'h  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'i'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'j  +75 h #t\Flushing upper manifold C'k& h &W C'l% h %4 .t\CSV.dial :flush C'm  +76 h  .t\CS.seek 5ml C'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'o @ +37 h @ C'p% h %p .t\CS.seek 7ml C'q'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'r +113 h .t\CTV.dial :air .\CS.seek 0ml C's'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +507 h '0 .t\CSV.dial :waste C'u +38 h .t\CS.seek 0ml C'v'4 +506 h '4 C'w& h &W C'x% h %4 .t\CSV.dial :flush C'y  +38 h  .t\CS.seek 3ml C'z'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'{ @ +38 h @ C'|% h %0 .t\CS.seek 5ml C'}'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'~'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C' ( +416 h ( M&\ & h &ӷ M& % h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t-> exit #m\EXIT @1534841321.47CEST @1535011875.96CEST = =tsimfast =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =msimfastScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2017 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi<; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> execute "18tun.rb" .GoodNews.suppressMail "Starting 18tun w/pucks in tube 2\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:11:24, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "18tun begun" #\Preparing to sleep until +0:00:15 #"shutdown"\Shutdown #t\Asleep til +0:00:15 #\Continuing at 01:00 E ;3\\0\\0 .tGoodNews.suppressMail "Then, in tube 2: coreNEG\\n/home/esp/esp2/mission/18tun.rb:106\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:11:41, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep until 01:00" +1499 E ;3\\0\\0 .tGate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ C' 2\ F*\ 9\ S)&\ .t\SPE.coast F*\ 9\ *\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig F*#,,'T(^20( b S)% h % .t\SS.configure SSconfig C'& S)#.0X 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:11:46, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Started 18tun phase coreNEG" #\Phase [coreNEG] #==================> STARTED coreNEG (phase #1) <=== #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'/& h & .tHand.open M&01 +150 h 1 M&1& h &_ M&2% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &3(- +166 h (- M&4& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &5& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &6'η +91 h 'η .tHand.close M&7/ +150 h / M&8& h &- M&9% h %η .t\Forearm.seek :retract M&:'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &;(ӷ +264 h (ӷ M&<& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &=& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &>' +91 h ' .t\CC.seek :closed C'?(W +416 h (W C'@& h &W .tHand.open M&A1 +150 h 1 M&B% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &C'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'D& h &W C'E% h %4 .t\CSV.dial CSR C'F  +150 h  C'G% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b 'H'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 'I% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 'J'[ +463 h '[ .t\CSV.dial :waste C'K +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'L'4 +2009 h '4 #t\Purging external Intake C'M& h &W C'N% h %4 .t\CSV.dial :flush C'O  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'P' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'Q 'R  h +75 .t\CTV.dial :intake C'S  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'T'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'U  +75 h #t\Flushing upper manifold C'V& h &W C'W% h %4 .t\CSV.dial :flush C'X  +75 h  .t\CS.seek 5ml C'Y'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'Z @ +37 h @ C'[% h %p .t\CS.seek 7ml C'\\'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'] +113 h .t\CTV.dial :air .\CS.seek 0ml C'^'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'_'0 +507 h '0 .t\CSV.dial :waste C'` +37 h .t\CS.seek 0ml C'a'4 +507 h '4 C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +38 h  .t\CS.seek 3ml C'e'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'f @ +38 h @ C'g% h %0 .t\CS.seek 5ml C'h'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'i'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'j'0 +507 h '0 .t\CSV.dial :waste C'k +75 h .t\CS.seek 0ml C'l'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&m& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &n(ӷ +7 h (ӷ M&o& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &p& h &ӷ .t\Forearm.seek CC M&q' +91 h ' .tHand.close M&r/ +150 h / .t\CC.seek :open C's( +416 h ( M&t& h &ӷ M&u% h % .t\Forearm.seek :retract M&v'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &w(- +264 h (- M&x& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &z'η +91 h 'η .tHand.open M&{1 +150 h 1 M&|% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &}'\ +91 h '\  #t\Starting mfb #pcrBAC .BACfans.suppressMail "Started mfb in tube 2 at 10:11:51CEST23-Aug-18\\n{1 => 0, 2 => 22, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:11:46, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "pcrBAC" F*~% #tLoading sh1 puck from tube 2 into Collection Clamp h %&K C'& #"readyMFB"Readying the MFB h & #"readyMFB"Homing MFB Syringe M&% ."readyMFB"\AFV.select :holdingCoil h %\  F* A(% h %X A(. h ., A(& h &  .t\SC.reconfigure SCloose A($.(\ 1~b (& h &  M&& h &- M&% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +6 F* % h %&K ."readyMFB"\ATV.dial :waste2 F*\ % +129 h % ."readyMFB"\AS.reconfigure ASconfig .\AS.home.seek :home F*#,,'T(^20( b * ' +31 h ( M& & h & A( & h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 20.71 Pucks below Top Plate A(' +57 h ' A(& h &  M&& h & .t\Elbow.seek 2 M&( +6 h ( M&& h & A(& h &  .t\Forearm.seek 2 + 300 counts M&'; +23 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& h & A('X M&% h %; .t\Forearm.seek :retract M&'\ +56 h 'X ."downElevator"\SE.coast A(5 +35 h '\  .t\Elbow.seek CC M&"(ӷ +142 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &(& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +91 h ' .t\CC.seek :closed C'+(W +416 h (W .tHand.open M&,1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  .t\SC.coast #skipping prime and sample collection A(6 #t\Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) .Intake.select :close C'%& h &W ."airStroke"\CSV.dial :waste S)'% ."airStroke"\CS.seek 0ml h % C'/'4 E ;3\\0\\0 #t\Calibrating pressure and temperature sensors .\CTV.dial :air .\SSV.dial :spit,avoiding: :exhaust .\SS.reconfigure SSfast .\SS.seek 5ml S)#.0X n +108 h n ."readyMFB"\AS.reconfigure ASnorm .\AS.home.seek :empty F*#,,'T20 b * 'R +889 C'I% h %4 S)H% h % #t\Evacuated: Intake @14.7psia, Outlet @14.7psia .\SSV.dial :spit,avoiding: :exhaust S)K +151 h .t\SS.reconfigure SSexhale .\SS.seek 0ml S)#.0Xd +100 C'39 h 9Td +100 C':9 h 9kd +100 C'.9 h 9d +100 C'@9 h 9d +100 C'A9 h 9d +100 C'89 h 9d +100 C'29 h 9d +100 C'D9 h 9d +100 C'E9 h 9 d +100 C'F9 h 9 d +100 C'=9 h 96d +100 C'>9 h 9Ld +100 C')9 h 9bd +100 C'I9 h 9xd +100 C'H9 h 9d +100 C'K9 h 9d +100 C'G9 h 9d +100 C'J9 h 9d +100 C'N9 h 9d +100 C'O9 h 9d +100 C'R9 h 9d +100 C'M9 h 9(d +100 C'S9 h 9>d +100 C'B9 h 9Td +100 C'Q9 h 9id +100 C'L9 h 9d +100 C'P9 h 9d +100 C'V9 h 9d +100 C'W9 h 9d +100 C'T9 h 9d +100 C'Z9 h 9d +100 C'U9 h 9  d +100 C'[9 h 9 d +100 C'Y9 h 9 *d +100 C'\\9 h 9 ?d +100 C'X9 h 9 Td +100 C']9 h 9 j d +100 C'`9 h 9 $d +100 C'_9 h 9 (d +100 C'b9 h 9 ,d +100 C'c9 h 9 0d +100 C'a9 h 9 4d +100 C'f9 h 9 8d +100 C'g9 h 9 % h %Z ."downElevator"\SE.seek :down A()'X +100 (I% h % M&H% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &K'\ +89 h '\  +82 'X ."downElevator"\SE.coast A(5 .t\SC.coast #Tranferring lysate to PRV .\CSV.dial :air C'G @ + A(6 +17 h @ .t\CS.seek 10ml C'J' +7 h ' .t\CSV.dial between air and CTV C'N ` +19 h ` .t\AIV.dial :bypass C'O { +72 h { #tBeginning samplePassC #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'R& h & .tHand.open M&M1 +2 h 1 M&S& h & M&B% h %\  .t\Elbow.seek FlushPuck::Garage M&Q(- +166 h (- M&L& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &- .t\Forearm.seek :garage M&V'η +91 h 'η .tHand.close M&W/ +150 h / M&T& h &- M&Z% h %η .t\Forearm.seek :retract M&U'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &[(ӷ +264 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &X' +91 h ' .t\CC.seek :closed C'](W +416 h (W C'`& h &W .tHand.open M&_1 +150 h 1 M&b% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &c'\ +91 h '\  .t\AWV.select :air .\AIV.dial :bypass .\PSV.dial PSR P%a +151 h P%f% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%g' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%e  +38 h  .t\PS.seek 0ml P%h' +1597 h ' .t\PSV.dial PSR P%^ +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%k' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV P%j  C'm  h +75 .t\PSV.dial :mixloop P%d +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing P%p  +113 h .t\PS.seek 0ml P%r' +2610 h ' .t\PSV.dial :air P%;  +37 h  .t\PS.seek 2.5ml P%o' +2610 h ' .t\PSV.dial :mixloop P%C +38 h .t\PS.seek 0ml P%t' +2609 h ' .t\PSV.dial :air P%u  +38 h  .t\PS.seek 2.5ml P%x' +2610 h ' .t\PSV.dial :mixloop P%? +37 h .t\PS.seek 0ml P%n' +2610 h ' #tGaraging FlushPuck in Collection Clamp M&i& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &y(ӷ +7 h (ӷ M&q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &{& h &ӷ .t\Forearm.seek CC M&v' +90 h ' .tHand.close M&~/ +150 h / .t\CC.seek :open C'|( +416 h ( M&& h &ӷ M&% h % .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &(- +265 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M& 1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b & '\ +91 h '\  #t\Connecting PS to CS to transfer sample .\CBV.dial AIV .\AIV.dial :bypass .\PSV.dial :mixloop .\PTV.dial :PRVmixing .\PRV.dial AIV .\CTV.dial :air,avoiding: :intake #Loading lyfil puck from tube 2 into Collection Clamp C' & h & M&\ % h %\  A(% h %X A(. h . .t\SC.reconfigure SCtight .\SC.seek 2 A($\ .(\ 1~b (( +200 h (  .t\SC.reconfigure SCloose A($.(\ 1~b (w& h &  M&z& h &- M&}% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 2 M&$&.,(\ #b &( +166 h ( M&& h & A(& h &  .t\Forearm.reconfigure TightForearm .\Forearm.seek 2 + 700 counts M&#Q@2^d^\ b &' +89 h ' A(& h &  .t\SE.seek 19.71 Pucks below Top Plate A(' +66 h ' A(& h &  M&#& h & .t\Elbow.seek 2 M&( +7 h ( M&& h & A((& h &  .t\Forearm.seek 2 + 300 counts M&'; +22 h '; .t\Forearm.reconfigure LooseForearm .Hand.close M&#Q@2^d^2b &/ +150 h / ."downElevator"\SE.seek :down M&& A(s'X h & M&,% h %; .t\Forearm.seek :retract M&'\ +66 h 'X ."downElevator"\SE.coast A(5 +25 h '\  .t\Elbow.seek CC M&%(ӷ +142 h (ӷ M&'& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &*& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &$' +91 h ' .t\CC.seek :closed C'4(W +416 h (W .tHand.open M&1 +150 h 1 M&5% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &"'\ +91 h '\  .t\SC.coast .\CTV.dial CSV,avoiding: :intake A(6 C'  +151 h  .t\CSV.dial CTV C'+  +19 h  .t\CS.seek 5ml C'7'p +1372 h 'p .tdelay 1:20 +8000 .\CS.seek 2.5ml C'0' +734 h '  .tdelay 40 seconds +4000 .\CS.seek 0ml C'9'4 +735 h '4 .tdelay 1:00 +6000 .\PSV.dial :air P%  +38 h  .t\PS.seek 2.5ml P%3' +2609 h ' .t\PSV.dial :mixloop P%: +38 h .tdelay 3 seconds +300 .\PSV.dial :air P%/  +38 h  .tdelay 2 seconds +200 .\PSV.dial :mixloop P%l +37 h .tdelay 3 seconds +300 .\PSV.dial :waste1 P%A  +151 h  .t\PS.seek :empty P%8' +2610 h ' .t\CBV.dial :waste1 C'2 @ +37 h @ .tdelay 5 seconds +500 .\CBV.dial AIV C'E  +38 h .tdelay 10 seconds +1000 .\CBV.dial :waste1 C'F @ +38 h @ .tdelay 10 seconds +1000 .\CBV.dial AIV C'>  +37 h .tdelay 10 seconds +1000 .\CTV.dial CSV,avoiding: :intake .\CBV.dial between AIV and waste1 C'6 ` +19 h ` .t\CSV.dial :air C'< @ +38 h @ .t\CS.seek 10ml C'I' +2647 h ' .t\CSV.dial CTV C'H  +38 h  .t\CS.seek 0ml C'.'4 +2647 h '4 .t\CBV.dial :waste1 C'D @ +19 h @ .tdelay 10 seconds +1000 .\CTV.dial :air,avoiding: :intake C'G  +150 h C'!& h &W #t\Storing lyfil puck in Collection Clamp to tube 1 M&N% h %\  A(O% h %X A(R. h . .t\SC.reconfigure SCtight .\SC.seek 1 A($\ .(\ 1~b (M( +200 h ( .t\SC.reconfigure SCloose A($.(\ 1~b (S& h & M&B& h &ӷ M&=% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &L( +143 h ( M&P& h & A()& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &1' +89 h ' A(& h & .t\SE.seek -0.29 Pucks below Top Plate A(@'\\ +265 h '\\ M&W& h & A(T& h & .t\Elbow.reconfigure LimpElbow .\Forearm.reconfigure LooseForearm M&$ ,(#b .t\Forearm.seek 1 M&#Q@2^d^2b &Z'Y +25 h 'Y .t\Elbow.hold M&K, +7 h , .t\Elbow.reconfigure TightElbow A(V- M&$&.,(\ #b h -+\\ .t\SE.seek 0.04 Pucks below Top Plate A(Y' +26 h ' M&\\% h %Y A(Q& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &U'υ +21 h 'υ M&&& h & A(`& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &_' +22 h ' .t\SE.seek 1.04 Pucks below Top Plate A(b' +36 h ' M&a& h & M&f% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +89 h '\  .t\Elbow.seek CC M&e(ӷ +143 h (ӷ M&g& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &^& h &ӷ .t\Forearm.seek CC M&h' +91 h ' .tHand.close M&j/ +150 h / .t\CC.seek :open C'm( +416 h ( A(d& h & A(p& h & M&k& h &ӷ M&;% h % .t\Forearm.seek :retract M&r'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek 1 M&$&.,(\ #b &C( +142 h ( M&o& h & A(u& h & .t\Elbow.reconfigure LimpElbow .\Forearm.seek 1 M&$ ,(#b &t'Y +93 h 'Y .t\Elbow.hold M&?, +6 h , .t\Elbow.reconfigure TightElbow .Hand.open M&$&.,(\ #b &x1 +150 h 1 M&i% h %Y A(q& h & .t\Forearm.force -20 M&5 .t\Forearm.seek 1 + 300 counts !Mo:I2C::Servo::Model::Warning:bt["=/home/esp/esp2/lib/i2c/servomodel.rb:606:in `applyForce'"5/home/esp/esp2/lib/rmutex.rb:85:in `synchronize'"=/home/esp/esp2/lib/i2c/servomodel.rb:604:in `applyForce'"=/home/esp/esp2/lib/i2c/servomodel.rb:146:in `applyForce'"5/home/esp/esp2/lib/i2c/servo.rb:461:in `process'"2/home/esp/esp2/lib/msg.rb:210:in `initialize'",/home/esp/esp2/lib/msg.rb:207:in `loop'"2/home/esp/esp2/lib/msg.rb:207:in `initialize'"7/home/esp/esp2/lib/schedule.rb:484:in `initialize'"1/home/esp/esp2/lib/threads.rb:718:in `start'"7/home/esp/esp2/lib/schedule.rb:477:in `initialize'"'/home/esp/esp2/lib/schedule.rb:475: mesg"+force -20% treated as 0% in simulation +2 &{'υ +21 h 'υ M&~& h & A([& h & .t\Forearm.reconfigure TightForearm .\Forearm.seek 1 + 700 counts M&#Q@2^d^\ b &c' +22 h ' A(]% h % ."downElevator"\SE.seek :down A('X +100 (% h %S M&v% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +89 h '\  +61 'X ."downElevator"\SE.coast A(5 .t\SC.coast .\PSV.dial :air P%  +2 A(6 +111 h  P%y% h % .t\PS.seek 0.499ml P% ' +585 h ' .tdelay 3 seconds +300 .\PSV.dial :mixloop P% +37 h .t\PRV.dial :air P%  +38 h  .tdelay 3 seconds +300 .\PRV.dial CBV P%  +38 h .t\CBV.dial AIV C'\  +37 h .t\PRV.dial between CBV and air P%  +19 h  .t\PRV.dial :air P%  +19 h  P%w% h % .t\PS.reconfigure PSslow .\PS.seek 0.599ml P%#A 2`xb %z' +250 h ' .t\PRV.dial :waste2 P%} @ +76 h @ .t\PS.reconfigure PSconfig .\PS.seek :empty P%#A d`xb %' +687 h ' #tLoading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h & M&% h %\  .t\Elbow.seek FlushPuck::Garage M&(- +166 h (- M&#& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&('η +91 h 'η .tHand.close M& / +150 h / M&& h &- M&% h %η .t\Forearm.seek :retract M&,'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +265 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &'& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &*' +90 h ' .t\CC.seek :closed C'(W +416 h (W C'& h &W .tHand.open M&s1 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  C'|& h &W #t\Clearing PML for modified lysate .\CTV.dial :air,avoiding: :intake .\PRV.dial :waste2 .\PTV.dial :PRV,avoiding: :puck P% @ +76 h @ .tdelay 2 seconds +200 .\PTV.dial :PRVmixing,avoiding: :puck P%5  +75 h .tdelay 2 seconds +200 .\PSV.dial :PML .delay 2 seconds +200 .\PRV.dial :air P%  +75 h  .tdelay 2 seconds +200 P%+% h % .t\PS.seek 1.499ml P%$' +1597 h ' .tdelay 5 seconds +500 .\PS.seek 2.5ml P%' +1094 h ' .t\PSV.dial :air P%J  +38 h  .t\PS.seek 0ml P%7' +2610 h ' #t\mixing lysate with speDil .\PSV.dial :PML P%0 +37 h .t\CBV.dial :PRV .\PRV.dial :air .delay 2 seconds +200 .\PRV.dial :speDil P%  +38 h  .tdelay 2 seconds +200 P%n% h % .t\PS.reconfigure PSslow1 .\PS.seek 0.225ml P%#A \ `xb %:'r +2310 h 'r .tdelay 5 seconds +500 .\PRV.dial CBV P%/  +75 h .tdelay 2 seconds +200 P%l% h %r .t\PS.seek 0.475ml P%A' +2563 h ' .tdelay 5 seconds +500 .\PRV.dial :air P%3  +38 h  .tdelay 2 seconds +200 .\PS.seek 0.965ml P%2'N +4990 h 'N .tdelay 10 seconds +1000 .\PRV.dial CBV P%E  +38 h .t\CTV.dial :air,avoiding: :intake .\PSV.dial :air P%F  +38 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %>' +1631 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%6 +76 h .t\PTV.dial :PRV,avoiding: :puck P%< @ +75 h @ .t\PS.seek 0ml P%9' +2610 h ' .t\PSV.dial :air P%H  +75 h  .t\PS.seek 2.5ml P%I' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial PTV P%D +75 h .t\PTV.dial :PRV,avoiding: :puck .\PS.seek 0ml P%G' +2610 h ' #t\Pushing modified lysate into AIV Injection Loop .\CBV.dial AIV .\AIV.dial :bypass .\PRV.dial CBV .\PSV.dial :air P%!  +75 h  .t\PS.seek 2.5ml P%N' +2610 h ' .tdelay 3 seconds +300 .\PSV.dial :PML P%O +37 h .t\PTV.dial :PRVmixing,avoiding: :puck P%R  +76 h P%S% h % .t\PS.reconfigure PSslow1 .\PS.seek 1.74ml P%#A \ `xb %B' +7722 h ' .tdelay 5 seconds +500 .\AIV.dial :sample C'= & +50 h & .t\AWV.select :waste C' +6 P%P% h % .t\PS.reconfigure PSslow2 .\PS.seek 1.22ml P%#A `xb %)'nC +10547 h 'nC .tdelay 15 seconds +1500 .\AIV.dial :bypass C' { +50 h { .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %%' +1313 h ' .t\PSV.dial :air P%"  +38 h  .t\PS.seek 2.5ml P%W' +2609 h ' .t\PSV.dial :PML P%T +38 h .t\PS.seek 0ml P%V' +2610 h ' .t\AIV.dial :coilToMFB .\AWV.select :air C' +6 '\\& h &W #"MFBPCR"\Extracting Nucleic Acid from AIV #tBeginning samplePassD ."MFBPCR"\SPE.hold 2:46:40,55C C'Q& F*& @@T h &W ."MFBPCR"\ASV.dial :SPEdelivery .t\AWV.select :air F*`  .t\AIV.dial :bypass .\PSV.dial PSR P%a +22 h  ."MFBPCR"delay 20 seconds +91 h P%f% h % #t\Pulling 1.5ml of PSR.killMFB .\PSR.select :killMFB P% +6 .t\PS.seek 1.5ml P%1' +1598 h ' .tdelay 5 seconds +305 #"MFBPCR"primeLysateINT .\AWV.select :air .\AIV.dial :coilToMFB .\ATV.dial AIV F*L I +129 h I F*@% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.299ml F*#,,'Td20 b *Z'6 +66 .t\PSV.dial :waste1 P%g  +37 h  .t\PS.seek 0ml P%^' +1597 h ' .t\PSV.dial PSR P%U +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%Y' +2610 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%_ +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%K' +1588 h '6 ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F*j  +22 h ' .t\PSV.dial PSR P%X +21 h  F*4% h %6 ."MFBPCR"\AS.seek 0.329ml F*d'ͯ +92 h P%p% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%k' +527 h 'ͯ ."MFBPCR"\ATV.dial :waste2 F*; % +22 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *o'R +1048 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%u  +38 h  .t\PS.seek 0ml P%h' +106 h 'R #"MFBPCR"Lysate primed! .\AFV.select :holdingCoil .\ATV.dial :air2 F*e  +22 h  F*x% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.069ml F*#,,'Td20 b *i' +1430 h ' ."MFBPCR"delay 3 seconds +39 h ' .t\PSV.dial PSR P%q +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%~' +217 ."MFBPCR"\ATV.dial :AIV F*[ I +43 h I F*]% h % ."MFBPCR"\AS.seek 0.469ml F*r' +2350 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P%C +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P%' +2609 h ' .t\PSV.dial PSR P%v +113 h P%t% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +7 .t\PS.seek 1.5ml P%{' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%b  +37 h  .t\PS.seek 0ml P%' +740 h ' ."MFBPCR"delay 5 seconds +500 F*y9 h 9n #"MFBPCR"\Solid Phase Extraction Heater at 55C .\ATV.dial ASV F*c n +22 h n ."MFBPCR"\ASV.dial :SPEdelivery F*% h % ."MFBPCR"\AS.seek 0.041ml F*M'% +336 h ' .t\PSV.dial PSR P%8 +37 h .t\PSR.select :air P% +7 .t\PS.seek 2.5ml P%' +2609 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.seek 0ml P% ' +2610 h ' .t\PSV.dial PSR P%\ +113 h P%% h % #t\Pulling 1.5ml of PSR.flush .\PSR.select :flush P%@ +6 .t\PS.seek 1.5ml P%' +1597 h ' .tdelay 5 seconds +500 .\PSV.dial :waste1 P%w  +38 h  .t\PS.seek 0ml P% ' +662 h '% ."MFBPCR"delay 10 seconds +935 h ' .t\PSV.dial PSR P%} +38 h .t\PSR.select :air P% +6 .t\PS.seek 2.5ml P%z' +21 #"MFBPCR"rinseLysateLineINT .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +2383 h ' ."connect"\PRV.dial CBV .t\CBV.dial PRV .\PSV.dial :mixloop P% +113 h .t\CTV.dial :air,avoiding: :intake .\PTV.dial :PRVmixing .\PS.reconfigure PSslow .\PS.seek 1.2ml P%#A 2`xb %'k +2678 h 'k .t\PSV.dial :waste1 P%  +151 h  .t\PS.reconfigure PSconfig .\PS.seek 0ml P%#A d`xb %#' +210 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial AIV F* I +22 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste C' +7 ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0ml F*#,,'T20 b *?'V +849 h ' .t\PSV.dial :air P%  +113 h  .t\PS.seek 2.5ml P%' +2610 h ' .t\PSV.dial :mixloop P% +37 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %' +5107 h ' .t\PSV.dial :air P%(  +37 h  .t\PS.reconfigure PSconfig .\PS.seek 2.5ml P%#A d`xb %' +1327 h 'V ."MFBPCR"\ATV.dial :air2 F*'  +42 h  F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.05ml F*#,,'Td20 b *,'Q +1027 h 'Q ."MFBPCR"\ATV.dial :AIV F*m I +43 h I F*% h %Q ."MFBPCR"\AS.seek 0.01ml F*s' +171 h ' .t\PSV.dial :mixloop P% +38 h .t\PS.reconfigure PSslow .\PS.seek 0ml P%#A 2`xb %|' +618 h ' ."MFBPCR"\ATV.dial :waste F* n +129 h n ."MFBPCR"\AS.seek :empty F**'R +238 h 'R #"MFBPCR"ethanol wash .\ATV.dial :air2 F*5  +86 h  F*% h %R ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.009ml F*#,,'T0 b *+' +1022 h ' ."MFBPCR"delay 3 seconds +300 #\Pulling 0.4ml of Ethanol Wash .\ATV.dial :ethanol F*  +43 h F*$% h % ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.409ml F*#,,'Td20 b *J'_ +2670 h ' #t\Cleaning Collection Syringe .\CSV.dial CSR C'  +38 h  C'0% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C' 'P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'n' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'7  '/  +38 h  +113  .t\CBV.dial :waste1 C'l @ +37 h @ .t\CS.seek 0ml C':'4 +1916 h '_ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air1 F*3  +21 h ."MFBPCR"delay 3 seconds +300 F*A% h %_ ."MFBPCR"\AS.seek 0.434ml F*E'ݏ +110 h '4 .t\CSV.dial CSR C'F  +38 h  C'2% h %4 #t\Pulling 4ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 4ml C'6'P +372 h 'ݏ ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ethanol F*<  +22 h F*>% h %ݏ ."MFBPCR"\AS.seek 0.419ml F*H'& +323 h '& ."MFBPCR"delay 3 seconds +99 h 'P .tdelay 5 seconds +201 ."MFBPCR"\ATV.dial ASV F*9 n +108 h n F*D% h %& ."MFBPCR"\AS.seek 0.092ml F*I'&0 +191 .t\CSR.select :air C' +7 .t\CS.seek 10ml C'!' +1625 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'G  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C'O'4 +2647 h '4 C'R& h &W #tGaraging FlushPuck in Collection Clamp M&S& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &N(ӷ +6 h (ӷ M&=& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &P& h &ӷ .t\Forearm.seek CC M&B' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C')( +416 h ( M&"& h &ӷ M&%% h % .t\Forearm.seek :retract M&T'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &W(- +265 h (- M&\\& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Q& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &`'η +90 h 'η .tHand.open M&a1 +150 h 1 M&f% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &V'\ +91 h '\  #t\Skipping Whole Cell Archive #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'L& h & .tHand.open M&@1 +2 h 1 M&g& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b & (- +7 h (- M&1& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &U& h &- .t\Forearm.seek :garage M&^'η +91 h 'η .tHand.close M&_/ +150 h / M&Y& h &- M&j% h %η .t\Forearm.seek :retract M&4'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &X(ӷ +264 h (ӷ M&p& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &Z& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &K' +91 h ' .t\CC.seek :closed C';(W +43 h '&0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :waste2 F*d % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *u'R +30 h (W C'e& h &W .tHand.open M&x1 +150 h 1 M&k% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &o'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'q& h &W C'[% h %4 .t\CSV.dial CSR C']  +38 h  C'h% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'i'p +245 h 'R ."MFBPCR"\ATV.dial :air2 F*C  +22 h  ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 1ml F*#,,'Td20 b *~' +1105 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 'v% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b 't'[ +462 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'b'4 +2010 h '4 #t\Flushing lower manifold's waste1 port C'{& h &W C'y% h %4 .t\CSV.dial :flush C'c  +37 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b ''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'r @ +37 h @ C'8% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste1 .\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C'\ & h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C'w'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'}% h %p .t\CS.seek 7ml C' 'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'M  +38 h  .t\CBV.dial :waste2b C'  +75 h  .t\CS.seek 0ml C'z'4 +766 h '4 #t\Flushing lower manifold's waste1 port .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +37 h  .t\CBV.dial :waste1 C'# @ +76 h @ .t\CS.seek 0ml C''4 +909 h '4 #t\Flushing lower manifold's waste2b port .\CSV.dial CSR C'  +38 h  C'(% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ''P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ''' +709 h ' ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'?  +75 h  .t\CS.seek 0ml C'm'4 +910 h '4 #tKilling upper manifold .\CSV.dial CSR C'  +38 h  C'% h %4 #t\Pulling 4ml of CSR.mfbkill .\CSR.select :mfbkill C'@ +6 .t\CS.reconfigure CSconfig .\CS.seek 4ml C'#P d@b ','P +1116 h 'P .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '' +710 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C' 's  +38 h +75 .t\CTV.dial :air C'5  +151 h .t\CS.seek 0ml C''4 +909 h '4 .tdelay 7 seconds +563 h ' ."MFBPCR"delay 5 seconds +137 .t\CS.seek 3ml C'*'0 +363 ."MFBPCR"\ATV.dial ASV F* n +64 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.seek 0.02ml F*$' +80 h '0 .t\CSV.dial :waste C'+ +37 h .t\CS.seek 0ml C''4 +507 h '4 #t\Flushing upper manifold C'0& h &W C'.% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.seek 5ml C'7'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'/ @ +38 h @ C'l% h %p .t\CS.seek 7ml C'n'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'3 +113 h .t\CTV.dial :air .\CS.seek 0ml C'A'4 +765 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'F'0 +507 h '0 .t\CSV.dial :waste C'2 +38 h .t\CS.seek 0ml C'J'4 +506 h '4 C':& h &W C'<% h %4 .t\CSV.dial :flush C'>  +38 h  .t\CS.seek 3ml C'E'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'9 @ +38 h @ C'D% h %0 .t\CS.seek 5ml C'H'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'6'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'G'0 +507 h '0 .t\CSV.dial :waste C'! +75 h .t\CS.seek 0ml C'R'4 +507 h '4 #t\Flushing lower manifold's waste1 port C'S& h &W C'=% h %4 .t\CSV.dial :flush C'P  +37 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'"  '%  +38 h  +113  .t\CBV.dial :waste1 C'B @ +38 h @ .t\CS.seek 0ml C'\\'4 +765 h '4 #t\Flushing lower manifold's waste2b port C'Q& h &W C'N% h %4 .t\CSV.dial :flush C'a  +76 h  .t\CS.seek 5ml C'f'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'T @ +38 h @ C'L% h %p .t\CS.seek 7ml C'@'i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'g  +37 h  .t\CBV.dial :waste2b C'1  +76 h  .t\CS.seek 0ml C'U'4 +766 h '4 #tGaraging FlushPuck in Collection Clamp M&`& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &_(ӷ +6 h (ӷ M&Y& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &j& h &ӷ .t\Forearm.seek CC M&W' +91 h ' .tHand.close M&V/ +150 h / .t\CC.seek :open C'^( +416 h ( M&p& h &ӷ M&Z% h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &4(- +264 h (- M&)& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &e'η +91 h 'η .tHand.open M&x1 +150 h 1 M&k% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &q'\ +91 h '\  #t\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'[& h & .tHand.open M&I1 +2 h 1 M&]& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &h(- +7 h (- M&K& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &- .t\Forearm.seek :garage M&O'η +91 h 'η .tHand.close M&u/ +150 h / M&v& h &- M&i% h %η .t\Forearm.seek :retract M&'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &t(ӷ +264 h (ӷ M&{& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &y& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &c' +91 h ' .t\CC.seek :closed C'b(W +416 h (W C'r& h &W .tHand.open M&81 +150 h 1 M&% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'& h &W C'\ % h %4 .t\CSV.dial CSR C'  +37 h  C' % h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '}'[ +463 h '[ .t\CSV.dial :waste C'w +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'M'4 +505 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial :air2 F*  +65 h  F* % h % ."MFBPCR"\AS.seek 0.52ml F*' +939 h '4 #t\Purging external Intake C'& h &W C'z% h %4 .t\CSV.dial :flush C'  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b ' ' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'# '  +37 h +38 .t\CTV.dial :intake C'  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'('4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'  +75 h #t\Flushing upper manifold C'& h &W C'% h %4 .t\CSV.dial :flush C'?  +75 h  .t\CS.seek 5ml C'''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C'm'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C', +113 h .t\CTV.dial :air .\CS.seek 0ml C's'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C'5 +37 h .t\CS.seek 0ml C''4 +507 h '4 C'& h &W C'% h %4 .t\CSV.dial :flush C'+  +38 h  .t\CS.seek 3ml C'~'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'* @ +38 h @ C'0% h %0 .t\CS.seek 5ml C'.'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C' '4 +650 h '4 .tdelay 7 seconds +85 h ' ."MFBPCR"delay 5 seconds +500 .\ATV.dial ASV F* n +65 h n ."MFBPCR"\ASV.dial :wasteR F*/  +21 h  ."MFBPCR"\AS.seek 0.3ml F*l': +29 .t\CS.seek 3ml C'7'0 +507 h '0 .t\CSV.dial :waste C'3 +75 h .t\CS.seek 0ml C'n'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&A& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &2(ӷ +7 h (ӷ M&F& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&<' +91 h ' .tHand.close M&>/ +150 h / .t\CC.seek :open C'J( +416 h ( M&9& h &ӷ M&D% h % .t\Forearm.seek :retract M&E'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &H(- +264 h (- M&6& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &!& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &G'η +91 h 'η .tHand.open M&S1 +150 h 1 M&=% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &P'\ +91 h '\  #t\Completed [coreNEG] begun 01:00:31 #\Extending 18tun until completion of MFBPCR +1978 h ': ."MFBPCR"delay 20 seconds +2000 .\AS.seek 0.07ml F*R'w +4647 h 'w ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste2 F* % +43 h % ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +433 h 'R #"MFBPCR"\Elution .\ATV.dial :air1 F*  +86 h F*"% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.049ml F*#,,'Td20 b *%'N +1027 h 'N ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F*B I +43 h I F*% h %N ."MFBPCR"\AS.seek 0.109ml F*Q'{ +1229 h '{ ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV,via: :air2 F*N n +172 h n ."MFBPCR"\ASV.dial :SPEelution F*a  +43 h ."MFBPCR"\AFV.select :carrierSolution F* +6 *\\% h %{ ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.759ml F*#,,'T20 b *T' ?x +3300 h ' ?x ."MFBPCR"delay 3 seconds +300 .\AFV.select :holdingCoil F* +7 *L% h % ?x ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.474ml F*#,,'Td20 b *f' +5753 h ' ."MFBPCR"delay 5 seconds +500 F*g% h % ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.439ml F*#,,'T0 b *1' +3537 h ' ."MFBPCR"delay 1:00 +6000 #pushing eluate to holding coil F*@% h % ."MFBPCR"\AS.seek 0.279ml F*`'Q +16105 h 'Q ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :waste,via: :air3 F*Y n +150 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *j'R +1635 h 'R #"MFBPCR"\Parking Bleach in LysateSource line and SPEcolumn .\ATV.dial :air3 F*V % +43 h % F*X% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.029ml F*#,,'Td20 b *o'v +625 h 'v ."MFBPCR"\ATV.dial :ElutionWater F*; I +21 h I F*p% h %v ."MFBPCR"\AS.seek 0.059ml F*Z'H +626 h 'H ."MFBPCR"\ATV.dial :air3 F*W % +21 h % F*)% h %H ."MFBPCR"\AS.seek 0.069ml F*d' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*_  +22 h F*x% h % ."MFBPCR"\AS.seek 0.769ml F*k' q +14098 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*[% h % q ."MFBPCR"\AS.seek 0.794ml F*I' k +525 h ' k ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*]  +22 h F*K% h % k ."MFBPCR"\AS.seek 0.779ml F*C'  +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :AIV F*e I +108 h I ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.015ml F*#,,'T(^20( b *u' +4416 h ' ."MFBPCR"\ATV.dial :waste F*v n +128 h n ."MFBPCR"\AS.seek :empty F*i'R +116 h 'R ."MFBPCR"\ATV.dial :air3 F*4 % +43 h % F*q% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *O' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*{  +22 h F*y% h % ."MFBPCR"\AS.seek 0.579ml F*h' +11485 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*^% h %  ."MFBPCR"\AS.seek 0.604ml F*r'\ 48 +525 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*8  +22 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*\ n +85 h n ."MFBPCR"\ASV.dial :wasteR F*  +43 h  ."MFBPCR"\AS.seek 0ml F* 'V +11871 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *c' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *U'V +20132 h 'V ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :air3 F* % +107 h % F*% h %V ."MFBPCR"\AS.seek 0.59ml F*w' +11887 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*} n +107 h n ."MFBPCR"\ASV.dial :wasteR F*% h %  ."MFBPCR"\AS.seek 0.09ml F* ' +10077 h ' ."MFBPCR"delay 30 seconds +3000 .\ATV.dial :extSrc F*$  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ElutionWater F* I +86 h I F*z% h % ."MFBPCR"\AS.seek 0.12ml F*' +625 h ' ."MFBPCR"\ATV.dial :air3 F*M % +22 h % F*% h % ."MFBPCR"\AS.seek 0.13ml F*#' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F* % h % ."MFBPCR"\AS.seek 0.23ml F*' +2033 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*% h % ."MFBPCR"\AS.seek 0.255ml F*?'X +526 h 'X ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*(  +21 h F*% h %X ."MFBPCR"\AS.seek 0.24ml F*' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F*' n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*,  +21 h  ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *m'(@ +1519 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 *s% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.17ml F*#,,'Td20 b *5'5 +6658 h '5 ."MFBPCR"\SPE.hold 2:46:40,95C F* @F@ h . ."MFBPCR"delay 3:00 +18000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +4794 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteD F* I +86 h I ."MFBPCR"\AS.seek 0ml F*+'V +5770 h 'V #"MFBPCR"\Priming Eluate .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F**  +43 h  #"MFBPCR"\Swish #1 of 2 F*0% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.6ml F*#,,'T20 b *~'\  +1031 h '\  F*.% h %\  ."MFBPCR"\AS.seek 0.5ml F*'(A +1032 h '(A #"MFBPCR"\Swish #2 of 2 F*/% h %(A ."MFBPCR"\AS.seek 0.6ml F*'\  +1032 h '\  F* % h %\  ."MFBPCR"\AS.seek 0.5ml F*3'(B +1031 h '(B ."MFBPCR"delay 3 seconds +300 F*7% h %(B ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.684ml F*#,,'Td20 b *A' M +3722 h ' M ."MFBPCR"delay 3 seconds +300 #\Eluate primed .\ATV.dial :waste2 F*F % +43 h % ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *:'V +3472 h 'V ."MFBPCR"\SPE.coast F*>  h  ."MFBPCR"\ASV.dial :SPErecovery #\Setting up PCR for Tt1 -- writing /var/log/esp/Tt170jan0102h.pcr #Building PCR Slug from ASV with MM2 mix, PP4 primer #\Priming with carrier .\ATV.dial ARV F*t  +65 h  ."MFBPCR"\ARV.dial :carrier F*  +10 h  F*b% h %V ."MFBPCR"\AS.seek 0.5ml F*9'(@ +2547 h '(@ ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *D' +2898 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F*< n +129 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *6'R +5065 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *!'(@ +2903 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 #"MFBPCR"Building Slug .\ATV.dial :ARV F*2  +129 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :air1 F*S  +22 h ."MFBPCR"delay 2 seconds +200 F*=% h %(@ ."MFBPCR"\AS.reconfigure ASslow2 .\AS.seek 0.506ml F*#,,'T0 b *E'Fj +620 h 'Fj ."MFBPCR"delay 2 seconds +200 .\ARV.dial :MM2 F*G  +129 h ."MFBPCR"delay 2 seconds +200 #\Adding MM2 F*l% h %Fj ."MFBPCR"\AS.seek 0.524ml F*' +1827 h ' ."MFBPCR"delay 25 seconds +2500 .\ARV.dial :PP4 F*R  +22 h  #"MFBPCR"\Adding PP4 .delay 2 seconds +200 F*% h % ."MFBPCR"\AS.seek 0.53ml F*"' +621 h ' ."MFBPCR"delay 10 seconds +1000 .\ARV.dial :air3 F* % +21 h % #"MFBPCR"adding ARV air .delay 2 seconds +200 F*B% h % ."MFBPCR"\AS.seek 0.536ml F*'< +621 h '< ."MFBPCR"delay 5 seconds +500 .\ARV.dial :carrier F*%  +107 h  ."MFBPCR"delay 7 seconds +700 F*N% h %< ."MFBPCR"\AS.seek 0.594ml F*a'\ +5849 h '\  ."MFBPCR"delay 5 seconds +500 .\ATV.dial :ASV F*\\ n +21 h n ."MFBPCR"delay 2 seconds +200 F*Q% h %\  ."MFBPCR"\AS.seek 0.6ml F*L'\  +621 h '\  ."MFBPCR"delay 4 seconds +400 .\ATV.dial ARV F*T  +21 h  ."MFBPCR"delay 2 seconds +200 .\ARV.dial :carrier .delay 2 seconds +200 F*g% h %\  ."MFBPCR"\AS.seek 0.65ml F*f' \\ +5044 h ' \\ ."MFBPCR"delay 4 seconds +400 .\ATV.dial PCR F*@  +43 h  ."MFBPCR"\ASV.dial :waste1 F*1  +64 h ."MFBPCR"\ARV.dial :air1 F*Y  +22 h #"MFBPCR"\Positioning Slug by 0.324ml F*`% h % \\ ."MFBPCR"\AS.seek 0.326ml F*V'{ +32596 h '{ ."MFBPCR"delay 5 seconds +500 .\ATV.dial ARV F*X  +43 h  ."MFBPCR"\ASV.dial :PCRlock F*j % +22 h % ."MFBPCR"delay 5 seconds +500 #Running Tt1 Profile60C300 thermocycling ."PCR"\PCR.program Profile60C300 #\Profile60C300 should complete in < 1:45:21 .\PCR.run 2:16:41.25 +632100 !o:#AnalyticalModule::PCRRawSample: @refi<; :\ @data""1:1,2,3,4,0.0;2:4,3,2,1,100.0 #"MFBPCR"\PCR for Tt1 completed -- closed /var/log/esp/Tt170jan0102h.pcr #\Full MFB Decontamination #Decon extraction column and decon AIV .\SPE.hold 2:46:40,55C F*; @@T ."MFBPCR"\ASV.dial :SPEdelivery F*p  +43 h  ."MFBPCR"delay 20 seconds +2000 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *o'(@ +1026 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :air1 F*W  +151 h F*)% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *Z' +423 h ' ."MFBPCR"\ATV.dial :ASV F*_ n +130 h n ."MFBPCR"\ASV.dial :SPErecovery F*x  +43 h  F*d% h % ."MFBPCR"\AS.seek 0.62ml F* '\ +2033 h '\  ."MFBPCR"\ATV.dial :waste F*[ n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *k'R +3153 h 'R ."MFBPCR"\AFV.select :holdingCoil .\ATV.dial :waste .\AS.reconfigure ASconfig .\AS.seek 0ml F*#,,'T(^20( b *]'V +28 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F*K'(@ +2898 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial :air3 F*I % +43 h % F*e% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.52ml F*#,,'Td20 b *C' +424 h ' ."MFBPCR"\ATV.dial :air3 F*v% h % ."MFBPCR"\AS.seek 0.53ml F*4' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*q  +21 h F*u% h % ."MFBPCR"\AS.seek 0.71ml F*i' H +3641 h ' H ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*{ % +22 h % ."MFBPCR"delay 3 seconds +300 F*y% h % H ."MFBPCR"\AS.seek 0.735ml F*O' Ű +525 h ' Ű ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*^% h % Ű ."MFBPCR"\AS.seek 0.72ml F*h' zG +324 h ' zG ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*8 n +86 h n ."MFBPCR"\ASV.dial :SPErecovery F*% h % zG ."MFBPCR"\AS.seek 0.365ml F*r' +7161 h ' ."MFBPCR"\ATV.dial :waste F*\ n +151 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek :empty F*#,,'T20 b *'R +1872 h 'R ."MFBPCR"\ATV.dial :air3 F* % +43 h % F* % h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *c' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.579ml F*U' +11484 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*} % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %  ."MFBPCR"\AS.seek 0.604ml F*w'\ 48 +526 h '\ 48 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*$  +21 h F*% h %\ 48 ."MFBPCR"\AS.seek 0.589ml F*z' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +86 h n ."MFBPCR"\ASV.dial :wasteR F*M  +22 h  ."MFBPCR"\AS.seek 0ml F*'V +11870 h 'V ."MFBPCR"delay 1:00 +6000 .\ATV.dial ASV .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ASV.dial :wasteR .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air3 F* % +108 h % F*#% h %V ."MFBPCR"\AS.seek 0.01ml F*' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +22 h F*% h % ."MFBPCR"\AS.seek 0.58ml F*(' q +11485 h ' q ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +21 h % ."MFBPCR"delay 3 seconds +300 F*?% h % q ."MFBPCR"\AS.seek 0.605ml F*''\ 8 +526 h '\ 8  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*,  +21 h F*% h %\ 8  ."MFBPCR"\AS.seek 0.59ml F*s' +324 h '  ."MFBPCR"delay 3 seconds +300 .\ATV.dial ASV F*m n +86 h n ."MFBPCR"\ASV.dial :SPEdelivery F*5  +21 h  ."MFBPCR"\AS.seek 0ml F*'V +11886 h 'V ."MFBPCR"delay 1:00 +6000 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\ATV.dial ASV .\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0ml F*#,,'Td20 b *+'V +20131 h 'V ."MFBPCR"delay 10 seconds +1000 .\ATV.dial :air2 F**  +65 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.8ml F*#,,'T20 b *0' x +8069 h ' x ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ASV F* n +65 h n ."MFBPCR"\ASV.dial :SPEdelivery .\AS.reconfigure ASslow1 .\AS.seek 0.03ml F*#,,'Td20 b *.'^ +15506 h '^ ."MFBPCR"delay 20 seconds +2000 .\ATV.dial :waste,via: :air3 F*~ n +151 h n ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b */'R +202 h 'R ."MFBPCR"\SPE.coast F*  h ' #"MFBPCR"Removing Bleach from Mixing Coil .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 0.5ml F* '(@ +2904 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial :ASV F* n +151 h n ."MFBPCR"\ASV.dial :SPErecovery F*7  +42 h  F*3% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.73ml F*#,,'Td20 b *F' +4648 h '  ."MFBPCR"\ASV.dial :wasteD F*A I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *>'V +3703 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *t'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*  +42 h  F*b% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *:': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*9  +64 h  F*<% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *D'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*6 n +65 h n F*2% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *S' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*= I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *!'V +2899 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *G'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"\ATV.dial ASV .\ASV.dial :SPErecovery F*l  +43 h  F*E% h %(@ ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *R': +2037 h ': ."MFBPCR"\ATV.dial :air2 F*  +64 h  F*% h %: ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.32ml F*#,,'Td20 b *'Q +424 h 'Q ."MFBPCR"\ATV.dial ASV F*B n +65 h n F*"% h %Q ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.57ml F*#,,'T20 b *%' + +2539 h ' + ."MFBPCR"\ASV.dial :wasteD F*N I +43 h I ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *'V +2899 h 'V ."MFBPCR"\ATV.dial :air2 F*\\  +64 h  ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 0.3ml F*#,,'T20 b *Q': +3043 h ': ."MFBPCR"\ATV.dial :ASV F*a n +65 h n ."MFBPCR"\ASV.dial :wasteD .\AS.reconfigure ASconfig .\AS.seek 0.05ml F*#,,'T(^20( b *T'Q +1463 h 'Q ."MFBPCR"\ATV.dial :waste F*g n +150 h n ."MFBPCR"\AS.seek 0ml F*L'V +313 h 'V ."MFBPCR"\ASV.dial :SPEdelivery F*@  +86 h  #"MFBPCR"deconLysateINT .\ATV.dial :air3 F*1 % +43 h % F*Y% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *`' +222 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*f  +22 h F*X% h % ."MFBPCR"\AS.seek 0.96ml F*j'0 +19125 h '0 ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*& % +22 h % ."MFBPCR"delay 3 seconds +300 F*p% h %0 ."MFBPCR"\AS.seek 0.985ml F*V' +525 h ' ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*W  +22 h F*)% h % ."MFBPCR"\AS.seek 0.97ml F*o'c +324 h 'c  ."MFBPCR"delay 3 seconds +300 .\ATV.dial AIV F*_ I +107 h I ."MFBPCR"\AIV.dial :coilToMFB .\AWV.select :waste .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *x' +198 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *d'R +10088 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *Z' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *['R +10087 h 'R ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b * ' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASslow0 .\AS.seek :empty F*#,,'T20 b *k'R +10088 h 'R ."MFBPCR"\ATV.dial :air2 F*]  +43 h  F*I% h %R ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.009ml F*#,,'Td20 b *e' +222 h ' ."MFBPCR"\AS.reconfigure ASslow0 .\AS.seek 1ml F*#,,'T20 b *K' +9988 h ' ."MFBPCR"\ATV.dial AIV F*v I +43 h I ."MFBPCR"\AS.seek 0.02ml F*C' +9880 h ' ."MFBPCR"\ATV.dial :waste F*q n +129 h n ."MFBPCR"\AS.seek 0ml F*u'V +227 h 'V ."MFBPCR"\AWV.select :air C' +6 F*4% h %V #"MFBPCR"\Cleaning PCR .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 0.5ml F*#,,'T(^20( b *{'(@ +2899 h '(@ ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"\ASV.dial :waste1 F*y  +21 h ."MFBPCR"\ATV.dial :air3 F*i % +43 h % F*% h %(@ ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.51ml F*#,,'Td20 b *^'Z +222 h 'Z ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*O  +22 h F*8% h %Z ."MFBPCR"\AS.seek 0.81ml F*'> +6055 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F*h % +22 h % ."MFBPCR"delay 3 seconds +300 F*\ % h %> ."MFBPCR"\AS.seek 0.835ml F*r'n +525 h 'n ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F* % h %n ."MFBPCR"\AS.seek 0.82ml F*'q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ARV.dial :waste F*  +43 h  ."MFBPCR"delay 3 seconds +300 .\ATV.dial :ARV F*  +64 h  F*c% h %q ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek 0.47ml F*#,,'T(^20( b *}'n +2037 h 'n ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*' +3071 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *U'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *$' +5770 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +7 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2722 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *w' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b * 'V +2722 h 'V ."MFBPCR"\ARV.dial between carrier and air1 F*M  +32 h  ."MFBPCR"\ATV.dial :air3 F*z % +86 h % F*% h %V ."MFBPCR"\AS.reconfigure ASslow1 .\AS.seek 0.01ml F*#,,'Td20 b *' +223 h ' ."MFBPCR"delay 2 seconds +200 .\ATV.dial :bleach F*  +21 h F*#% h % ."MFBPCR"\AS.seek 0.81ml F*'> +16110 h '> ."MFBPCR"delay 3 seconds +300 .\ATV.dial :air3 F* % +22 h % ."MFBPCR"delay 3 seconds +300 F*% h %> ."MFBPCR"\AS.seek 0.835ml F*'m +525 h 'm ."MFBPCR"delay 3 seconds +300 .\ATV.dial :bleach F*  +22 h F*?% h %m ."MFBPCR"\AS.seek 0.82ml F*('q +324 h 'q ."MFBPCR"delay 3 seconds +300 .\ATV.dial PCR F*,  +21 h  ."MFBPCR"\AS.reconfigure ASconfig .\AS.seek :empty F*#,,'T(^20( b *'R +4741 h 'R ."MFBPCR"\PCR.program :bleachProfile #\PCR program should complete in < 2:45 .\PCR.run 10:00 +16500 .\AFV.select :carrierSolution F* +7 ."MFBPCR"delay 2 seconds +200 .\AS.seek 1ml F*'' +5775 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *m'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *5' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *s'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *'V +2723 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b **' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASfast .\AS.seek 0ml F*#,,'T(20( b *+'V +2722 h 'V ."MFBPCR"delay 3 seconds +300 .\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\AS.reconfigure ASnorm .\AS.seek 0ml F*#,,'T20 b *0'V +5060 h 'V ."MFBPCR"\AFV.select :carrierSolution F* +6 ."MFBPCR"delay 2 seconds +200 .\AS.reconfigure ASconfig .\AS.seek 1ml F*#,,'T(^20( b *~' +5771 h ' ."MFBPCR"delay 1 second +100 .\AFV.select :holdingCoil F* +6 ."MFBPCR"delay 1 second +100 .\ATV.dial :waste F* n +87 h n ."MFBPCR"\AS.reconfigure ASnorm .\AS.seek 0.5ml F*#,,'T20 b *.'(@ +2547 h '(@ ."MFBPCR"\ATV.dial :waste2 F*/ % +108 h % ."MFBPCR"\AS.seek 0ml F*'V +2547 h 'V #t\Asleep after completing 18tun assays .GoodNews.suppressMail "{1 => 2, 2 => 20, 3 => 22, 4 => 22, 5 => 22, 6 => 22, 7 => 22}\\nCTD data unavailable\\nCan@10:11:54, 20.0C, 50% humidity, 14.7psia, 12.001V, 0.298A, 0.247A avg, 3.57W",Subject: "Asleep after completing 18tun assays" #\Mission runtime was 1:41.066682 #-> exit #m\EXIT @1535012017.69CEST @1537114903.18CEST = =tsimfast =hcore =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =msimfastScheduler =ECan @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi$; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ *\ 9\ C' 2\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% h % F*#,,'T(^20( b .t\SS.configure SSconfig C'& h & .t\CC.configure CCconfig S)#.0X% h %\  M&?& h &M A(@& h &  A(A% h %X C'B9 h 9 P%C9 h 9 #t-> initialPurge #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'D& h & .tHand.open M&E1 +150 h 1 M&F& h &M M&G% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &H(- +166 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &K'η +91 h 'η .tHand.close M&L/ +150 h / M&M& h &- M&N% h %η .t\Forearm.seek :retract M&O'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &P(ӷ +264 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &S' +91 h ' .t\CC.seek :closed C'T(W +416 h (W C'U& h &W .tHand.open M&V1 +150 h 1 M&W% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'Y& h &W C'Z% h %4 .t\CSV.dial CSR C'[  +151 h  C'\\% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ']'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '^% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '_'[ +462 h '[ .t\CSV.dial :waste C'` +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'a'4 +2010 h '4 #t\Purging external Intake C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'e' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'f 'g  h +75 .t\CTV.dial :intake C'h  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'i'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'j  +75 h #t\Flushing upper manifold C'k& h &W C'l% h %4 .t\CSV.dial :flush C'm  +76 h  .t\CS.seek 5ml C'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'o @ +37 h @ C'p% h %p .t\CS.seek 7ml C'q'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'r +113 h .t\CTV.dial :air .\CS.seek 0ml C's'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +507 h '0 .t\CSV.dial :waste C'u +38 h .t\CS.seek 0ml C'v'4 +506 h '4 C'w& h &W C'x% h %4 .t\CSV.dial :flush C'y  +38 h  .t\CS.seek 3ml C'z'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'{ @ +38 h @ C'|% h %0 .t\CS.seek 5ml C'}'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'~'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C' ( +416 h ( M&\ & h &ӷ M& % h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t-> exit #m\EXIT @1537115042.87CEST @1537968118.44CEST = =FMFB =Pprocessing =Ssampler =Astorage =Ccollection =Mmanipulator =ECan =msimfastScheduler =tsimfast =hcore @CET0.00 #t\Simulated gateway v3.24 for core bus #\Copyright 2018 MBARI\ /home/esp/esp2/type/shallow/mer/configure.rb\ #\ Configuration for MFB3 by preston@mbari.org\ /home/esp/esp2/type/mfb/MFB3/configure.rb\ !o:AnalyticalModule::PCRCore: @configS:Instrument::Configuration: name"PCR:calFileName0:sampleCmd" DATA?\ : prompt0:statusCmd" STATUS?\ :sampleTimeouti:promptTimeouti\ :statusTimeouti\ : retriesi:strToPrompt0: pace0: echoT: @refi; :\ @name@ E ;3\\0\\0  ;3\\0\\0 #t\Polling Can every 10:00 #-> ESP.ready! .Gate.power :core,:ON .Gate.power :raw,:ON +119 S) ) h  C' ' h  P% % h  F* * h  C' 9\ ' 0\ ' -\ P% 9\ % 2\ % *\ % 0\ S)&\ F*\ 9\ C' 2\ F*\ 9\ .t\SPE.coast F*\ 9\ *  h  .t\SPE.configure SPEconfig F*\ 2 *% h % .t\AS.configure ASconfig S)% F*#,,'T(^20( b h % .t\SS.configure SSconfig C'& S)#.0X% h %\  M&?& h &M A(@& h &  A(A% h %X C'B9 h 9 P%C9 h 9 #t-> initialPurge #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'D& h & .tHand.open M&E1 +150 h 1 M&F& h &M M&G% h %\  .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &H(- +166 h (- M&I& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &J& h &- .t\Forearm.reconfigure LooseForearm .\Forearm.seek :garage M&#Q@2^d^2b &K'η +91 h 'η .tHand.close M&L/ +150 h / M&M& h &- M&N% h %η .t\Forearm.seek :retract M&O'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &P(ӷ +264 h (ӷ M&Q& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &R& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &S' +91 h ' .t\CC.seek :closed C'T(W +416 h (W C'U& h &W .tHand.open M&V1 +150 h 1 M&W% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &X'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'Y& h &W C'Z% h %4 .t\CSV.dial CSR C'[  +151 h  C'\\% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ']'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 '^% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '_'[ +462 h '[ .t\CSV.dial :waste C'` +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b 'a'4 +2010 h '4 #t\Purging external Intake C'b& h &W C'c% h %4 .t\CSV.dial :flush C'd  +37 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b 'e' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'f 'g  h +75 .t\CTV.dial :intake C'h  +76 h  .tIntake.select :open S) +6 .t\CS.seek 0ml C'i'4 +910 h '4 .tIntake.select :close S)0 +6 .t\CTV.dial :air C'j  +75 h #t\Flushing upper manifold C'k& h &W C'l% h %4 .t\CSV.dial :flush C'm  +76 h  .t\CS.seek 5ml C'n'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'o @ +37 h @ C'p% h %p .t\CS.seek 7ml C'q'i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'r +113 h .t\CTV.dial :air .\CS.seek 0ml C's'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C't'0 +507 h '0 .t\CSV.dial :waste C'u +38 h .t\CS.seek 0ml C'v'4 +506 h '4 C'w& h &W C'x% h %4 .t\CSV.dial :flush C'y  +38 h  .t\CS.seek 3ml C'z'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'{ @ +38 h @ C'|% h %0 .t\CS.seek 5ml C'}'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'~'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C''0 +507 h '0 .t\CSV.dial :waste C' +75 h .t\CS.seek 0ml C''4 +507 h '4 #tGaraging FlushPuck in Collection Clamp M&& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &(ӷ +6 h (ӷ M&& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &ӷ .t\Forearm.seek CC M&' +91 h ' .tHand.close M&/ +150 h / .t\CC.seek :open C' ( +416 h ( M&\ & h &ӷ M& % h % .t\Forearm.seek :retract M& '\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b & (- +264 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &'η +91 h 'η .tHand.open M&1 +150 h 1 M&% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &'\ +91 h '\  #t-> initialPurge #\Starting initialPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'& h & .tHand.open M&1 +2 h 1 M&& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &(- +7 h (- M&& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b && h &- .t\Forearm.seek :garage M&'η +91 h 'η .tHand.close M&/ +150 h / M&"& h &- M&% h %η .t\Forearm.seek :retract M&('\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &*(ӷ +264 h (ӷ M&+& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &,& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &' +91 h ' .t\CC.seek :closed C'(W +416 h (W C'%& h &W .tHand.open M&'1 +150 h 1 M&/% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &0'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'1& h &W C'2% h %4 .t\CSV.dial CSR C'3  +150 h  C'4% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b '5'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '6% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b '7'[ +463 h '[ .t\CSV.dial :waste C'8 +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '9'4 +2009 h '4 #t\Purging external Intake C':& h &W C';% h %4 .t\CSV.dial :flush C'<  +38 h  .t\CS.reconfigure CSfast .\CS.seek 10ml C'#d @b '=' +910 h ' .tdelay 5 seconds +500 ."connect"\CSV.dial CBV .t\CBV.dial CSV C'> +75 h .t\CTV.dial :intake C'?  +75 h  .tIntake.select :open S) +7 .t\CS.seek 0ml C'@'4 +909 h '4 .tIntake.select :close S)0 +7 .t\CTV.dial :air C'A  +75 h #t\Flushing upper manifold C'B& h &W C'C% h %4 .t\CSV.dial :flush C'D  +75 h  .t\CS.seek 5ml C'E'p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C'F @ +37 h @ C'G% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CBV .t\CBV.dial CSV C'I +113 h .t\CTV.dial :air .\CS.seek 0ml C'J'4 +766 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'&'0 +507 h '0 .t\CSV.dial :waste C'L +37 h .t\CS.seek 0ml C'M'4 +507 h '4 C'N& h &W C')% h %4 .t\CSV.dial :flush C'K  +38 h  .t\CS.seek 3ml C'Q'0 +506 h '0 .tdelay 5 seconds +500 .\CSV.dial :air C'R @ +38 h @ C'H% h %0 .t\CS.seek 5ml C'O'p +416 h 'p .t\CSV.dial :air .\CS.seek 0ml C'.'4 +650 h '4 .tdelay 7 seconds +700 .\CS.seek 3ml C'U'0 +507 h '0 .t\CSV.dial :waste C'V +75 h .t\CS.seek 0ml C'W'4 +506 h '4 #tGaraging FlushPuck in Collection Clamp M&Y& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &Z(ӷ +7 h (ӷ M&[& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &\\& h &ӷ .t\Forearm.seek CC M&S' +91 h ' .tHand.close M& / +150 h / .t\CC.seek :open C'^( +416 h ( M&]& h &ӷ M&`% h % .t\Forearm.seek :retract M&_'\ +91 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &b(- +264 h (- M&c& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &d& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &a'η +91 h 'η .tHand.open M&g1 +150 h 1 M&f% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &h'\ +91 h '\  #t-> finalPurge #\Starting finalPurge #Loading FlushPuck into Collection Clamp from FlushPuck::Garage C'e& h & .tHand.open M&j1 +2 h 1 M&k& h &- .t\Elbow.reconfigure TightElbow .\Elbow.seek FlushPuck::Garage M&$&.,(\ #b &l(- +7 h (- M&m& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &i& h &- .t\Forearm.seek :garage M&o'η +91 h 'η .tHand.close M&p/ +150 h / M&n& h &- M&r% h %η .t\Forearm.seek :retract M&q'\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &s(ӷ +265 h (ӷ M&u& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &t& h &ӷ .t\Forearm.reconfigure TightForearm .\Forearm.seek CC M&#Q@2^d^\ b &w' +91 h ' .t\CC.seek :closed C'x(W +416 h (W C'y& h &W .tHand.open M&v1 +150 h 1 M&{% h % .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &|'\ +91 h '\  #t\Flushing the Collection Valve::Manifold C'z& h &W C'}% h %4 .t\CSV.dial CSR C'~  +150 h  C'% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +7 .t\CS.reconfigure CSconfig .\CS.seek 5ml C'#P d@b ''p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +6 '% h %p .t\CS.reconfigure CSfast .\CS.seek 7.5ml C'#d @b ''[ +463 h '[ .t\CSV.dial :waste C' +151 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b ''4 +2009 h '4 #t\Flushing lower manifold's waste1 port C'P& h &W C'X% h %4 .t\CSV.dial :flush C'T  +38 h  .t\CS.reconfigure CSfast .\CS.seek 5ml C'#d @b '\ 'p +650 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +38 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  '  +37 h  +113  .t\CBV.dial :waste1 C' @ +38 h @ .t\CS.seek 0ml C''4 +766 h '4 #t\Flushing lower manifold's waste2b port C' & h &W C'% h %4 .t\CSV.dial :flush C'  +75 h  .t\CS.seek 5ml C''p +651 h 'p .tdelay 5 seconds +500 .\CSV.dial :air C' @ +37 h @ C'% h %p .t\CS.seek 7ml C''i +416 h 'i ."connect"\CSV.dial CTV .t\CTV.dial CSV C'  +38 h  .t\CBV.dial :waste2b C'"  +75 h  .t\CS.seek 0ml C''4 +766 h '4 #tDisinfecting external Intake with kill C' & h &W C'% h %4 .t\CSV.dial CSR C'  +38 h  C'+% h %4 #t\Pulling 8ml of CSR.kill .\CSR.select :kill C' +6 .t\CS.seek 8ml C','L +816 h 'L .tdelay 5 seconds +500 .\CSR.select :air C' +6 .t\CS.seek 10ml C'' +416 h ' ."connect"\CSV.dial CBV .t\CBV.dial CSV C'( '  +38 h +75 .t\CTV.dial :intake C'%  +75 h  .tIntake.select :open S) +7 .t\CS.seek 2ml C''' +816 h ' .tIntake.select :close S)0 +6 .t\CTV.dial :air C'/  +75 h .t\CS.reconfigure CSconfig .\CS.seek 0ml C'#P d@b '1'4 +607 h '4 .t\CSV.dial CSR C'2  +113 h  C'3% h %4 #t\Pulling 5ml of CSR.flush .\CSR.select :flush C' +6 .t\CS.seek 5ml C'4'p +1372 h 'p .tdelay 5 seconds +500 .\CSR.select :air C' +7 .t\CS.seek 10ml C'' +1372 h ' .t\CSV.dial CBV C' +113 h .t\CS.seek 0ml C'6'4 +2647 h '4 #tGaraging FlushPuck in Collection Clamp M&5& h &ӷ .t\Elbow.reconfigure TightElbow .\Elbow.seek CC M&$&.,(\ #b &8(ӷ +7 h (ӷ M&7& h &ӷ .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &:& h &ӷ .t\Forearm.seek CC M&;' +91 h ' .tHand.close M&& h &ӷ M&?% h % .t\Forearm.seek :retract M&='\ +90 h '\  .t\Elbow.reconfigure TightElbow .\Elbow.seek :garage M&$&.,(\ #b &A(- +265 h (- M&B& h &- .t\Elbow.reconfigure LooseElbow M&$ &.,(#b &C& h &- .t\Forearm.reconfigure TightForearm .\Forearm.seek :garage M&#Q@2^d^\ b &D'η +91 h 'η .tHand.open M&@1 +150 h 1 M&F% h %η .t\Forearm.reconfigure LooseForearm .\Forearm.seek :retract M&#Q@2^d^2b &G'\ +91 h '\  #t-> exit #m\EXIT @1537969765.75CEST