@01:00:00.00CET01-Jan-70 <quick> Simulated gateway v3.24 for core bus
Copyright 2018 MBARI
/home/esp/esp2/type/shallow/mer/configure.rb

Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
Polling Can every 10:00
Loading /home/esp/esp2/mission/190905totalPostcanqc.rb mission
Preparing to sleep until +0:00:15
<shutdown> Shutdown
<quick> Asleep  til +0:00:15
Continuing at 01:00
@01:00:16.50 All dwarf controllers running firmware version 3.99
@01:00:31.57 Phase [mbaPhase]
==================>  STARTED MBAsuperphase (phase #1)  <===
@01:00:31.58CET01-Jan-70 Readying the MFB
Homing MFB Syringe
@01:02:45.84 Starting mfb
pcrBAC sampling at most 100ml, wcr at most 100ml
Loading sh1 puck from tube 2 into Collection Clamp
@01:03:00.60 collectSample 100ml w/bubble point of 55psi
Priming 7ml
@01:03:00.67 Calibrating pressure and temperature sensors
@01:03:14.06 Waiting for external sampler to finish priming...
@01:08:30.41 Sampling 100.0ml
@01:14:49.74 Sampled  100.0ml
Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds)
@01:15:37.49 BubblePoint: Intake @14.7psia, Outlet @14.7psia
@01:15:57.79 Evacuated: Intake @14.7psia, Outlet @14.7psia
@01:16:25.85 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds)
@01:17:12.11 BubblePoint: Intake @14.7psia, Outlet @14.7psia
@01:17:32.41 Evacuated: Intake @14.7psia, Outlet @14.7psia
@01:18:00.46 Lysing: Adding 1.5ml lysis
@01:18:04.42 Pulling 1ml of CSR.lysis
@01:18:38.88 Pulling 1.5ml of CSR.lysis
@01:19:08.95 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00
@01:20:07.95 <mwj> Session Begun
-> showlog 10
-> exit
Session Ended
@01:26:02.95 <quick> Collection Puck Heater at 85C after lysing
@01:26:45.63 Recovering Lysate into Collection syringe
@01:27:27.40 Lysing Complete
Storing sh1 puck in Collection Clamp to tube 1
@01:27:40.86 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation
@01:27:44.01 Tranferring lysate to PRV
@01:27:45.18 Beginning samplePassC
Loading FlushPuck into Collection Clamp from FlushPuck::Garage
@01:28:01.81 Pulling 1.5ml of PSR.flush
@01:31:20.73 Garaging FlushPuck in Collection Clamp
@01:31:34.23 Connecting PS to CS to transfer sample
Loading lyfil puck from tube 2 into Collection Clamp
@01:38:06.45 Storing lyfil puck in Collection Clamp to tube 1
@01:38:14.00 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation
@01:38:28.52 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation
@01:38:56.46 Loading FlushPuck into Collection Clamp from FlushPuck::Garage
@01:39:11.58 Clearing PML for modified lysate
@01:40:20.23 mixing lysate with speDil
@01:44:13.11 Pushing modified lysate into AIV Injection Loop
@01:49:13.99 <MFBPCR> Extracting Nucleic Acid from AIV
<quick> Beginning samplePassD
@01:49:15.12 Pulling 1.5ml of PSR.killMFB
@01:49:34.21 <MFBPCR> primeLysateINT
@01:50:47.39 <quick> Pulling 1.5ml of PSR.flush
@01:51:09.87 <MFBPCR> Lysate primed!
@01:52:19.67 <quick> Pulling 1.5ml of PSR.flush
@01:52:53.48 <MFBPCR> Solid Phase Extraction Heater at 55C
@01:53:51.95 <quick> Pulling 1.5ml of PSR.flush
@01:54:29.98 <MFBPCR> rinseLysateLineINT
@01:57:33.60 ethanol wash
@01:57:47.68 Pulling 0.4ml of Ethanol Wash
@01:58:14.81 <quick> Cleaning Collection Syringe
@01:58:15.19 Pulling 4ml of CSR.flush
@01:59:16.46 Pulling 4ml of CSR.flush
@02:00:15.85 Garaging FlushPuck in Collection Clamp
@02:00:29.35 Whole Cell Archive sampling 100ml
Loading wcr puck from tube 2 into Collection Clamp
@02:00:46.28 collectSample 100ml w/bubble point of 55psi
Priming 7ml
@02:00:46.34 Calibrating pressure sensors
@02:01:01.24 Waiting for external sampler to finish priming...
@02:06:16.09 Sampling 100.0ml
@02:11:38.37 <MFBPCR> Elution
@02:12:35.41 <WCR> Sampled  100.0ml
Drying puck before fixing for 4:00
@02:13:45.64 Pumping wcr puck from tube 2 up to 55psi...
@02:15:14.89 Fix #1 of 2 (with rnal) for 2:00
@02:15:15.26 Pulling 2ml of CSR.rnal
@02:15:15.33 <mwj> Session Begun
-> showlog 10
-> showlog 10
-> showlog 10
-> exit
Session Ended
@02:15:24.39 <MFBPCR> pushing eluate to holding coil
@02:15:34.61 <WCR> Pumping wcr puck from tube 2 up to 55psi...
@02:18:53.30 <MFBPCR> Parking Bleach in LysateSource line and SPEcolumn
@02:19:04.99 <WCR> Fix #2 of 2 (with rnal) for 2:00
@02:19:05.36 Pulling 2ml of CSR.rnal
@02:19:24.72 Pumping wcr puck from tube 2 up to 55psi...
@02:22:38.24 <mwj> Session Begun
-> showlog 10
-> exit
Session Ended
@02:22:55.09 <WCR> Storing wcr puck in Collection Clamp to tube 1
@02:23:02.54 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation
@02:23:17.05 I2C::Servo::Model::Warning in manipulator -- force -20% treated as 0% in simulation
@02:23:19.91 Starting finalPurge
Loading FlushPuck into Collection Clamp from FlushPuck::Garage
@02:23:19.93 <mwj> Session Begun
-> showlog 10
-> exit
Session Ended
@02:23:35.03 <quick> Flushing the Collection Valve::Manifold
@02:23:35.40 Pulling 5ml of CSR.flush
@02:24:20.48 Flushing lower manifold's waste1 port
@02:24:46.82 Flushing lower manifold's waste2b port
@02:25:12.40 Flushing lower manifold's waste1 port
@02:25:12.77 Pulling 4ml of CSR.mfbkill
@02:25:46.38 Flushing lower manifold's waste2b port
@02:25:46.76 Pulling 4ml of CSR.mfbkill
@02:26:20.37 Killing upper manifold
@02:26:20.75 Pulling 4ml of CSR.mfbkill
@02:27:13.37 Flushing upper manifold
@02:28:35.45 Flushing lower manifold's waste1 port
@02:29:01.40 Flushing lower manifold's waste2b port
@02:29:26.98 Garaging FlushPuck in Collection Clamp
@02:29:40.49 Starting initialPurge
Loading FlushPuck into Collection Clamp from FlushPuck::Garage
@02:29:54.02 Flushing the Collection Valve::Manifold
@02:29:54.39 Pulling 5ml of CSR.flush
@02:30:39.47 Purging external Intake
@02:31:05.43 Flushing upper manifold
@02:32:27.88 Garaging FlushPuck in Collection Clamp
@02:32:41.39 Completed [mbaPhase] begun 01:00:31
Extending 190905totalPostcanqc until completion of MFBPCR
@02:44:12.31 <MFBPCR> Priming Eluate
@02:44:44.86 Swish #1 of 2
@02:45:05.49 Swish #2 of 2
@02:46:09.34 Eluate primed
@02:46:44.49 Setting up PCR for Pp -- writing /var/log/esp/Pp70jan0102h100ml.pcr
Building PCR Slug from ASV with MM1 mix, PP2 primer
Priming with carrier
@02:49:07.92 Building Slug
@02:49:24.07 Adding MM1
@02:50:07.56 Adding PP2
@02:50:25.98 adding ARV air
@02:53:03.11 Positioning Slug by 0.324ml
@02:58:39.71 Running Pp Profile60C350 thermocycling
<PCR> Profile60C350 should complete in < 1:48:05
<mwj> Session Begun
-> showlog 10
-> showlog 10
-> exit
Session Ended
@04:46:44.71 <MFBPCR> PCR for Pp completed -- closed /var/log/esp/Pp70jan0102h100ml.pcr
Cleaning PCR
@04:49:23.30 <mwj> Session Begun
-> showlog 10
-> showlog 10
-> exit
Session Ended
@04:56:44.97 <MFBPCR> PCR program should complete in < 2:45
@05:05:06.95 <mwj> Session Begun
-> showlog 10
-> exit
Session Ended
@05:07:30.67 <MFBPCR> Setting up PCR for Ss -- writing /var/log/esp/Ss70jan0105h100ml.pcr
Building PCR Slug from ASV with MM2 mix, PP4 primer
Priming with carrier
@05:09:53.89 Building Slug
@05:10:10.89 Adding MM2
@05:10:54.38 Adding PP4
@05:11:12.80 adding ARV air
@05:12:39.78 <mwj> Session Begun
-> showlog 10
-> exit
Session Ended
@05:13:49.29 <MFBPCR> Positioning Slug by 0.324ml
@05:19:25.89 Running Ss Profile60C350 thermocycling
<PCR> Profile60C350 should complete in < 1:48:05
@07:07:30.89 <MFBPCR> PCR for Ss completed -- closed /var/log/esp/Ss70jan0105h100ml.pcr
Cleaning PCR
@07:09:05.04 <mwj> Session Begun
-> showlog 10
-> showlog 10
-> exit
Session Ended
@07:17:31.14 <MFBPCR> PCR program should complete in < 2:45
@07:28:16.84 Setting up PCR for Mm -- writing /var/log/esp/Mm70jan0107h100ml.pcr
Building PCR Slug from ASV with MM2 mix, PP3 primer
Priming with carrier
@07:30:40.06 Building Slug
@07:30:57.07 Adding MM2
<mwj> Session Begun
-> showlog 20
-> exit
Session Ended
@07:31:40.55 <MFBPCR> Adding PP3
@07:31:58.97 adding ARV air
@07:34:35.46 Positioning Slug by 0.324ml
@07:40:12.06 Running Mm Profile60C350 thermocycling
<PCR> Profile60C350 should complete in < 1:48:05
@09:28:17.06 <MFBPCR> PCR for Mm completed -- closed /var/log/esp/Mm70jan0107h100ml.pcr
Cleaning PCR
@09:34:27.67 <mwj> Session Begun
-> showlog 10
-> exit
Session Ended
@09:38:17.32 <MFBPCR> PCR program should complete in < 2:45
@09:49:03.01 Setting up PCR for IPc -- writing /var/log/esp/IPc70jan0109h100ml.pcr
Building PCR Slug from ASV with MM1 mix, PP1 primer
Priming with carrier
@09:50:03.43 <mwj> Session Begun
-> showlog 10
-> exit
Session Ended
@09:51:26.24 <MFBPCR> Building Slug
@09:51:42.38 Adding MM1
@09:52:25.86 Adding PP1
@09:52:44.29 adding ARV air
@09:55:19.92 Positioning Slug by 0.324ml
@10:00:56.52 Running IPc ProfileIPc thermocycling
<PCR> ProfileIPc should complete in < 1:50:49
@11:51:45.52 <MFBPCR> PCR for IPc completed -- closed /var/log/esp/IPc70jan0109h100ml.pcr
Full MFB Decontamination
Decon extraction column and decon AIV
@12:20:43.23 Removing Bleach from Mixing Coil
@12:27:19.60 deconLysateINT
@12:41:35.61 Cleaning PCR
@12:51:54.17 PCR program should complete in < 2:45
@13:04:34.58 <quick> Asleep after completing 190905totalPostcanqc assays
Mission runtime was 14:02.220403
@01:00:00.00CET01-Jan-70 <quick> Simulated gateway v3.24 for core bus
Copyright 2018 MBARI
/home/esp/esp2/type/shallow/mer/configure.rb

Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
Polling Can every 10:00
Cleanly exited at 01:01:16CET01-Jan-70
@01:01:16.00 Loading /home/esp/esp2/mission/TotalTRIAL.rb mission
Preparing to sleep until +00:01:00
<shutdown> Shutdown
<quick> Asleep  til +00:01:00
Continuing at 01:02
@01:02:17.50 All dwarf controllers running firmware version 3.99
@01:02:32.57 Phase [wcrPhase2]
@01:02:32.58 Completed [wcrPhase2] begun 01:02:32
Asleep after completing TotalTRIAL assays
Mission runtime was 5.423787 seconds
@01:00:00.00CET01-Jan-70 <quick> Simulated gateway v3.24 for core bus
Copyright 2018 MBARI
/home/esp/esp2/type/shallow/mer/configure.rb

Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
Polling Can every 10:00
Cleanly exited at 01:02:32CET01-Jan-70
@01:02:32.00 Loading /home/esp/esp2/mission/TotalTRIAL.rb mission
Preparing to sleep until +00:01:00
<shutdown> Shutdown
<quick> Asleep  til +00:01:00
Continuing at 01:03
@01:03:33.50 All dwarf controllers running firmware version 3.99
@01:03:48.57 Phase [wcrPhase2]
@01:03:48.58 Completed [wcrPhase2] begun 01:03:48
Asleep after completing TotalTRIAL assays
Mission runtime was 5.295949 seconds
@01:00:00.00CET01-Jan-70 <quick> Simulated gateway v3.24 for core bus
Copyright 2018 MBARI
/home/esp/esp2/type/shallow/mer/configure.rb

Configuration for MFB3 by preston@mbari.org
/home/esp/esp2/type/mfb/MFB3/configure.rb
Polling Can every 10:00
Cleanly exited at 01:03:48CET01-Jan-70
@01:03:48.00 Loading /home/esp/esp2/mission/TotalTRIAL.rb mission
Preparing to sleep until +00:01:00
<shutdown> Shutdown
<quick> Asleep  til +00:01:00
Continuing at 01:04
@01:04:49.50 All dwarf controllers running firmware version 3.99
@01:05:04.57 Phase [wcrPhase2]
@01:05:04.58 Completed [wcrPhase2] begun 01:05:04
Asleep after completing TotalTRIAL assays
Mission runtime was 5.397966 seconds