@18:14:22.39CEST11-May-20
ESP2 v3.30 I2C Gateway v3.06 for core bus @18:14:24.79 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @18:14:25.08 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @18:14:31.13 Polling Can every 10:00 @18:14:33.01 SPE.coast @18:14:33.07 SPE.configure SPEconfig @18:14:33.16 AS.reconfigure ASconfig @18:14:33.21 AS.hold @18:14:33.26 AS.configure ASconfig @18:14:33.36 SS.reconfigure SSconfig @18:14:33.40 SS.hold @18:14:33.45 SS.configure SSconfig @18:14:33.64 CC.reconfigure CCconfig @18:14:33.73 CC.hold @18:14:33.84 CC.configure CCconfig @18:14:34.03 CS.reconfigure CSconfig @18:14:34.11 CS.hold @18:14:34.24 CS.configure CSconfig @18:14:34.82 PC.reconfigure PCconfig @18:14:34.86 PC.hold @18:14:34.93 PC.configure PCconfig @18:14:35.02 PS.configure PSconfig @18:14:35.14 Forearm.reconfigure LooseForearm @18:14:35.18 Forearm.hold @18:14:35.23 Forearm.configure LooseForearm @18:14:35.33 Elbow.reconfigure LooseElbow @18:14:35.37 Elbow.hold @18:14:35.43 Elbow.configure LooseElbow @18:14:35.55 SC.configure SCloose @18:14:35.64 SE.configure SEconfig @18:14:35.68 CH.coast @18:14:35.74 CH.configure CHconfig @18:14:35.78 PH.coast @18:14:35.84 PH.configure PHconfig @18:14:35.87 PCR.off @18:14:35.91 PCR.pidtemp= 50,0.1,0,300,25,25 @18:14:35.94 PCR.caltemp= 2.8814,0.0372,0 @18:14:35.97 All dwarf controllers running firmware version 3.75 @18:14:36.11 Loading /home/esp/esp2/mission/20invasivedeplopostcanqc.rb mission @18:14:36.18 Preparing to sleep until +0:00:05 @18:14:36.20 GoodNews.email "Starting 20invasivedeplopostcanqc w/pucks in tube 2\n{}\nCan@18:14:31, 17.0C, 15% humidity, 14.7psia, 11.996V, 0.575A, 0.563A avg, 6.90W",Subject: "20invasivedeplopostcanqc begun" @18:14:36.31 AS.coast @18:14:36.36
Asleep til +0:00:05 @18:14:36.37 SS.coast @18:14:36.40
Continuing at 18:14 @18:14:36.54 CC.coast @18:14:36.65 CS.coast @18:14:36.73 PC.coast @18:14:36.80 Forearm.coast @18:14:36.88 Elbow.coast @18:14:37.32 Session Begun @18:14:37.46 Gate.power :core,:OFF @18:14:37.49 Gate.power :raw,:OFF @18:14:37.51 Shutdown @18:14:41.14
Powering core bus @18:14:43.60 -> showlog 10 @18:14:45.12 GoodNews.email "Then, in tube 2: mbaPhase\n/home/esp/esp2/mission/20invasivedeplopostcanqc.rb:119\n{}\nCan@18:14:36, 17.1C, 15% humidity, 14.7psia, 11.996V, 0.544A, 0.544A avg, 6.52W",Subject: "Asleep until 18:14" @18:14:46.05
Gate.power :raw,:ON @18:14:47.44 SPE.coast @18:14:47.55 SPE.configure SPEconfig @18:14:47.74 AS.configure ASconfig @18:14:47.90 SS.configure SSconfig @18:14:48.05 CC.configure CCconfig @18:14:48.25 CS.configure CSconfig @18:14:48.52 PC.configure PCconfig @18:14:48.81 PS.configure PSconfig @18:14:49.17 Forearm.configure LooseForearm @18:14:49.41 Elbow.configure LooseElbow @18:14:49.67 SC.configure SCloose @18:14:50.02 SE.configure SEconfig @18:14:50.15 CH.coast @18:14:50.39 CH.configure CHconfig @18:14:50.51 PH.coast @18:14:50.75 PH.configure PHconfig @18:14:51.07 PCR.off @18:14:51.25 PCR.pidtemp= 50,0.1,0,300,25,25 @18:14:51.40 PCR.caltemp= 2.8814,0.0372,0 @18:14:51.51 All dwarf controllers running firmware version 3.75 @18:14:51.73 Valve.reset @18:14:52.20 PCR.off @18:14:52.30 PCR.pidtemp= 50,0.1,0,300,25,25 @18:14:52.44 PCR.caltemp= 2.8814,0.0372,0 @18:14:53.05 Intake.select :close @18:14:53.20 Exhaust.select :close @18:14:53.28 CTV.dial :air @18:14:53.52 PSV.dial :air @18:14:53.59 CSV.dial :waste1 @18:14:54.24 SSV.dial :spit,avoiding: :exhaust @18:14:54.37 PS.reconfigure PSconfig @18:14:54.47
Forearm.reconfigure LooseForearm @18:14:54.94 PS.home.seek 0ml @18:14:55.05
Forearm.home.seek :retract @18:14:57.13 Hand.close @18:14:57.37
Elbow.jog 1500 @18:14:57.51 SS.reconfigure SSconfig @18:14:57.69 CS.reconfigure CSfast @18:14:58.21 SS.home.seek 0ml @18:14:58.35 CS.home.seek 0ml @18:14:58.97
Elbow.reconfigure LooseElbow @18:14:59.16 Elbow.home.seek :clear @18:15:04.14 SE.reconfigure SEconfig @18:15:04.41 SE.home.seek :down @18:15:04.54 PS.coast @18:15:05.68
SC.reconfigure SCsloppy @18:15:05.91 SC.home.seek :initial @18:15:17.95 Forearm.seek :retract @18:15:18.32 Elbow.seek :hammer @18:15:19.74 Forearm.reconfigure TightForearm @18:15:19.97 Forearm.seek 5 + 350 counts @18:15:21.37 Forearm.seek :retract @18:15:23.28 PC.reconfigure PCconfig @18:15:23.34
CC.reconfigure CCconfig @18:15:23.69 PC.home.seek :open @18:15:23.79
CC.home.seek :open @18:15:28.22 Phase [mbaPhase] @18:15:28.27 GoodNews.email "At 18:15:27 began in tube 2: mbaPhase\n/home/esp/esp2/mission/20invasivedeplopostcanqc.rb:119\n{}\nCan@18:15:28, 17.2C, 15% humidity, 14.7psia, 11.937V, 0.752A, 0.752A avg, 8.98W",Subject: "Started 20invasivedeplopostcanqc phase mbaPhase" @18:15:28.38CEST11-May-20
===> STARTED Superphase (phase #1) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @18:15:28.87 Starting mfb @18:15:28.92 pcrBAC sampling at most 100ml, wcr at most 100ml @18:15:29.33 Loading sh1 puck from tube 2 into Collection Clamp @18:15:29.83 Readying the MFB @18:15:29.89
Hand.open @18:15:29.99 Homing MFB Syringe @18:15:30.21 AFV.select :holdingCoil @18:15:30.78 ATV.dial :air2 @18:15:32.32
SC.reconfigure SCloose @18:15:32.54 AS.reconfigure ASconfig @18:15:33.42 AS.home.seek :home @18:15:34.13
Elbow.reconfigure TightElbow @18:15:34.42 Elbow.seek 2 @18:15:35.76 Forearm.seek 2 + 700 counts @18:15:37.23 SE.reconfigure SEpick @18:15:37.51 SE.seek beamBreak @18:15:38.02 BACfans.email "Started mfb in tube 2 at 18:15:29CEST11-May-20\n{}\nCan@18:15:28, 17.2C, 15% humidity, 14.7psia, 11.937V, 0.752A, 0.752A avg, 8.98W",Subject: "pcrBAC sampling at most 100ml, wcr at most 100ml" @18:15:39.12
SE.configure SEconfig @18:15:39.96 AFV.select :carrierSolution @18:15:40.69
Elbow.seek 2 @18:15:41.14 delay 2 seconds @18:15:42.12
Forearm.seek 2 + 300 counts @18:15:42.82 Forearm.reconfigure LooseForearm @18:15:43.31 Hand.close @18:15:43.86 AS.seek 1ml @18:15:44.78 SE.seek :down @18:15:46.51
Forearm.seek :retract @18:15:46.85 SE.coast @18:15:47.79
Elbow.seek CC @18:15:50.01 Elbow.reconfigure LooseElbow @18:15:50.62 Forearm.reconfigure TightForearm @18:15:50.96 Forearm.seek CC @18:15:54.01 CC.seek :closed @18:19:38.25 I2C::Gateway::Reset in core_reader -- Received BREAK @18:19:55.96 Polling Can every 10:00 @18:20:57.94 delay 1 second @18:20:59.06 Session Ended @18:21:00.21 Errno::EPIPE in bkha's_reader -- Broken pipe @18:21:01.13 Session Begun @18:21:01.48 Errno::EPIPE in cmdSocket -- Broken pipe @18:21:02.08 Session Begun @18:21:03.51 -> d @18:21:03.63 AFV.select :holdingCoil @18:21:03.71 NameError in bkha#2 -- undefined local variable or method `d' for # @18:21:08.80 I2C::Bus::Error in core_reader -- No Response from core @18:21:14.01 I2C::Gateway::Reset in readyMFB -- Restarted ESP2 v3.30 I2C Gateway v3.06 @18:21:14.94 I2C::Gateway::Reset in MAIN -- Restarted ESP2 v3.30 I2C Gateway v3.06 @18:21:15.24 Retry #1 of 3 @18:21:15.89
Retry #1 of 3 @18:21:16.43 delay 1 second @18:21:16.80 Polling Can every 10:00 @18:21:20.55 ATV.dial :waste @18:21:25.63 I2C::Bus::Error in core_reader -- No Response from core @18:21:26.77 I2C::Gateway::Reset in readyMFB -- Restarted ESP2 v3.30 I2C Gateway v3.06 @18:21:27.15 I2C::Gateway::Reset in MAIN -- Restarted ESP2 v3.30 I2C Gateway v3.06 @18:21:27.40 Retry #1 of 3 @18:21:27.59
Retry #2 of 3 @18:21:28.25 Polling Can every 10:00 @18:21:28.61 AS.reconfigure ASnorm @18:21:33.80 I2C::Bus::Error in core_reader -- No Response from core @18:21:34.91 I2C::Gateway::Reset in readyMFB -- Restarted ESP2 v3.30 I2C Gateway v3.06 @18:21:35.23 I2C::Gateway::Reset in MAIN -- Restarted ESP2 v3.30 I2C Gateway v3.06 @18:21:35.33 Retry #1 of 3 @18:21:35.59
Retry #3 of 3 @18:21:36.41 Polling Can every 10:00 @18:21:36.57 AS.home.seek :empty @18:21:41.94 I2C::Bus::Error in core_reader -- No Response from core @18:21:43.31 I2C::Gateway::Reset in readyMFB -- Restarted ESP2 v3.30 I2C Gateway v3.06 @18:21:43.67 Puck::Warning in MAIN -- Retaining claim on Arm because Hand is likely holding a puck! @18:21:43.92 Retry #1 of 3 @18:21:44.09 I2C::Gateway::Reset in MAIN -- Restarted ESP2 v3.30 I2C Gateway v3.06 @18:21:44.60
Waiting to Resume from Checkpoint "Restarted ESP2 v3.30 I2C Gateway v3.06" @18:21:44.75 Polling Can every 10:00 @18:21:45.30 BadNews.email "Restarted ESP2 v3.30 I2C Gateway v3.06",Subject: "I2C::Gateway::Reset in MAIN" @18:22:29.61 ATV.dial :waste @18:22:29.74 ARV.dial between carrier and air1 @18:22:30.44 ASV.dial :waste1 @18:26:33.54 -> showlog 400 @18:36:23.59 -> @18:15:47.79
Elbow.seek CC @18:36:23.68 SyntaxError in aaa -- compile error aaa:2: `@1' is not allowed as an instance variable name @18:36:23.79 -> @18:15:50.01 Elbow.reconfigure LooseElbow @18:36:23.81 SyntaxError in aaa -- compile error aaa:3: `@1' is not allowed as an instance variable name @18:36:23.92 -> @18:15:50.62 Forearm.reconfigure TightForearm @18:36:23.93 SyntaxError in aaa -- compile error aaa:4: `@1' is not allowed as an instance variable name @18:36:24.03 -> @18:15:50.96 Forearm.seek CC @18:36:24.04 SyntaxError in aaa -- compile error aaa:5: `@1' is not allowed as an instance variable name @18:36:24.14 -> @18:15:54.01 CC.seek :closed @18:36:24.15 SyntaxError in aaa -- compile error aaa:6: `@1' is not allowed as an instance variable name @18:36:24.28 -> @18:19:38.25 I2C::Gateway::Reset in core_reader -- Received BREAK @18:36:24.29 SyntaxError in aaa -- compile error aaa:7: `@1' is not allowed as an instance variable name @18:36:24.55 -> @18:19:55.96 Polling Can every 10:00 @18:36:24.57 SyntaxError in aaa -- compile error aaa:8: `@1' is not allowed as an instance variable name @18:36:24.69 -> @18:20:57.94 delay 1 second @18:36:24.70 SyntaxError in aaa -- compile error aaa:9: `@1' is not allowed as an instance variable name @18:36:24.81 -> @18:20:59.06 Session Ended @18:36:24.83 SyntaxError in aaa -- compile error aaa:10: `@1' is not allowed as an instance variable name @18:36:24.97 -> @18:21:00.21 Errno::EPIPE in bkha's_reader -- Broken pipe @18:36:24.98 SyntaxError in aaa -- compile error aaa:11: `@1' is not allowed as an instance variable name @18:36:25.09 -> @18:21:01.13 Session Begun @18:36:25.11 SyntaxError in aaa -- compile error aaa:12: `@1' is not allowed as an instance variable name @18:36:25.24 -> @18:21:01.48 Errno::EPIPE in cmdSocket -- Broken pipe @18:36:25.26 SyntaxError in aaa -- compile error aaa:13: `@1' is not allowed as an instance variable name @18:36:25.38 -> @18:21:02.08 Session Begun @18:36:25.40 SyntaxError in aaa -- compile error aaa:14: `@1' is not allowed as an instance variable name @18:36:25.48 -> @18:21:03.51 -> d @18:36:25.50 SyntaxError in aaa -- compile error aaa:15: `@1' is not allowed as an instance variable name @18:36:25.63 -> @18:21:03.63 AFV.select :holdingCoil @18:36:25.65 SyntaxError in aaa -- compile error aaa:16: `@1' is not allowed as an instance variable name @18:36:25.85 -> @18:21:03.71 NameError in bkha#2 -- undefined local variable or method `d' for # @18:36:25.86 SyntaxError in aaa -- compile error aaa:17: `@1' is not allowed as an instance variable name @18:36:26.02 -> @18:21:08.80 I2C::Bus::Error in core_reader -- No Response from core @18:36:26.03 SyntaxError in aaa -- compile error aaa:18: `@1' is not allowed as an instance variable name @18:36:26.21 -> @18:21:14.01 I2C::Gateway::Reset in readyMFB -- Restarted ESP2 v3.30 I2C Gateway v3.06 @18:36:26.23 SyntaxError in aaa -- compile error aaa:19: `@1' is not allowed as an instance variable name @18:36:26.38 -> @18:21:14.94 I2C::Gateway::Reset in MAIN -- Restarted ESP2 v3.30 I2C Gateway v3.06 @18:36:26.40 SyntaxError in aaa -- compile error aaa:20: `@1' is not allowed as an instance variable name @18:36:26.49 -> @18:21:15.24 Retry #1 of 3 @18:36:26.50 SyntaxError in aaa -- compile error aaa:21: `@1' is not allowed as an instance variable name @18:36:26.64 -> @18:21:15.89
Retry #1 of 3 @18:36:26.67 SyntaxError in aaa -- compile error aaa:22: `@1' is not allowed as an instance variable name @18:36:26.79 -> @18:21:16.43 delay 1 second @18:36:26.80 SyntaxError in aaa -- compile error aaa:23: `@1' is not allowed as an instance variable name @18:36:26.91 -> @18:21:16.80 Polling Can every 10:00 @18:36:26.93 SyntaxError in aaa -- compile error aaa:24: `@1' is not allowed as an instance variable name @18:36:27.03 -> @18:21:20.55 ATV.dial :waste @18:36:27.05 SyntaxError in aaa -- compile error aaa:25: `@1' is not allowed as an instance variable name @18:36:27.17 -> @18:21:25.63 I2C::Bus::Error in core_reader -- No Response from core @18:36:27.19 SyntaxError in aaa -- compile error aaa:26: `@1' is not allowed as an instance variable name @18:36:27.33 -> @18:21:26.77 I2C::Gateway::Reset in readyMFB -- Restarted ESP2 v3.30 I2C Gateway v3.06 @18:36:27.34 SyntaxError in aaa -- compile error aaa:27: `@1' is not allowed as an instance variable name @18:36:27.48 -> @18:21:27.15 I2C::Gateway::Reset in MAIN -- Restarted ESP2 v3.30 I2C Gateway v3.06 @18:36:27.50 SyntaxError in aaa -- compile error aaa:28: `@1' is not allowed as an instance variable name @18:36:27.59 -> @18:21:27.40 Retry #1 of 3 @18:36:27.61 SyntaxError in aaa -- compile error aaa:29: `@1' is not allowed as an instance variable name @18:36:27.70 -> @18:21:27.59
Retry #2 of 3 @18:36:27.72 SyntaxError in aaa -- compile error aaa:30: `@1' is not allowed as an instance variable name @18:36:27.83 -> @18:21:28.25 Polling Can every 10:00 @18:36:27.84 SyntaxError in aaa -- compile error aaa:31: `@1' is not allowed as an instance variable name @18:36:27.95 -> @18:21:28.61 AS.reconfigure ASnorm @18:36:27.97 SyntaxError in aaa -- compile error aaa:32: `@1' is not allowed as an instance variable name @18:36:28.09 -> @18:21:33.80 I2C::Bus::Error in core_reader -- No Response from core @18:36:28.11 SyntaxError in aaa -- compile error aaa:33: `@1' is not allowed as an instance variable name @18:36:28.24 -> @18:21:34.91 I2C::Gateway::Reset in readyMFB -- Restarted ESP2 v3.30 I2C Gateway v3.06 @18:36:28.26 SyntaxError in aaa -- compile error aaa:34: `@1' is not allowed as an instance variable name @18:36:28.40 -> @18:21:35.23 I2C::Gateway::Reset in MAIN -- Restarted ESP2 v3.30 I2C Gateway v3.06 @18:36:28.42 SyntaxError in aaa -- compile error aaa:35: `@1' is not allowed as an instance variable name @18:36:28.50 -> @18:21:35.33 Retry #1 of 3 @18:36:28.52 SyntaxError in aaa -- compile error aaa:36: `@1' is not allowed as an instance variable name @18:36:28.62 -> @18:21:35.59
Retry #3 of 3 @18:36:28.65 SyntaxError in aaa -- compile error aaa:37: `@1' is not allowed as an instance variable name @18:36:28.76 -> @18:21:36.41 Polling Can every 10:00 @18:36:28.78 SyntaxError in aaa -- compile error aaa:38: `@1' is not allowed as an instance variable name @18:36:28.89 -> @18:21:36.57 AS.home.seek :empty @18:36:28.90 SyntaxError in aaa -- compile error aaa:39: `@1' is not allowed as an instance variable name @18:36:29.03 -> @18:21:41.94 I2C::Bus::Error in core_reader -- No Response from core @18:36:29.05 SyntaxError in aaa -- compile error aaa:40: `@1' is not allowed as an instance variable name @18:36:29.18 -> @18:21:43.31 I2C::Gateway::Reset in readyMFB -- Restarted ESP2 v3.30 I2C Gateway v3.06 @18:36:29.19 SyntaxError in aaa -- compile error aaa:41: `@1' is not allowed as an instance variable name @18:36:29.35 -> @18:21:43.67 Puck::Warning in MAIN -- Retaining claim on Arm because Hand is likely holding a puck! @18:36:29.36 SyntaxError in aaa -- compile error aaa:42: `@1' is not allowed as an instance variable name @18:36:29.45 -> @18:21:43.92 Retry #1 of 3 @18:36:29.47 SyntaxError in aaa -- compile error aaa:43: `@1' is not allowed as an instance variable name @18:36:29.61 -> @18:21:44.09 I2C::Gateway::Reset in MAIN -- Restarted ESP2 v3.30 I2C Gateway v3.06 @18:36:29.63 SyntaxError in aaa -- compile error aaa:44: `@1' is not allowed as an instance variable name @18:36:29.78 -> @18:21:44.60
Waiting to Resume from Checkpoint "Restarted ESP2 v3.30 I2C Gateway v3.06" @18:36:29.80 SyntaxError in aaa -- compile error aaa:45: `@1' is not allowed as an instance variable name @18:36:29.92 -> @18:21:44.75 Polling Can every 10:00 @18:36:29.93 SyntaxError in aaa -- compile error aaa:46: `@1' is not allowed as an instance variable name @18:36:30.10 -> @18:21:45.30 BadNews.email "Restarted ESP2 v3.30 I2C Gateway v3.06",Subject: "I2C::Gateway::Reset in MAIN" @18:36:30.12 SyntaxError in aaa -- compile error aaa:47: `@1' is not allowed as an instance variable name @18:36:30.22 -> @18:22:29.61 ATV.dial :waste @18:36:30.24 SyntaxError in aaa -- compile error aaa:48: `@1' is not allowed as an instance variable name @18:36:30.34 -> @18:22:29.74 ARV.dial between carrier and air1 @18:36:30.35 SyntaxError in aaa -- compile error aaa:49: `@1' is not allowed as an instance variable name @18:36:30.45 -> @18:22:30.44 ASV.dial :waste1 @18:36:30.47 SyntaxError in aaa -- compile error aaa:50: `@1' is not allowed as an instance variable name @18:36:30.56 -> @18:26:33.54 -> showlog 400 @18:36:30.57 SyntaxError in aaa -- compile error aaa:51: `@1' is not allowed as an instance variable name @18:36:30.68 -> @18:15:47.79
Elbow.seek CC @18:36:30.74 SyntaxError in aaa -- compile error aaa:52: `@1' is not allowed as an instance variable name UserAbort in aaa -- @14:53:47.67CEST14-May-20
ESP2 v3.30 I2C Gateway v3.06 for core bus @14:53:50.12 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @14:53:50.43 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @14:53:56.00 Polling Can every 10:00 @14:53:57.82 SPE.coast @14:53:57.88 SPE.configure SPEconfig @14:53:57.99 AS.configure ASconfig @14:53:58.09 SS.reconfigure SSconfig @14:53:58.13 SS.hold @14:53:58.18 SS.configure SSconfig @14:53:58.28 CC.reconfigure CCconfig @14:53:58.32 CC.hold @14:53:58.39 CC.configure CCconfig @14:53:58.49 CS.reconfigure CSconfig @14:53:58.53 CS.hold @14:53:58.61 CS.configure CSconfig @14:53:58.71 PC.reconfigure PCconfig @14:53:58.75 PC.hold @14:53:59.18 PC.configure PCconfig @14:53:59.27 PS.configure PSconfig @14:53:59.39 Forearm.reconfigure LooseForearm @14:53:59.44 Forearm.hold @14:53:59.49 Forearm.configure LooseForearm @14:53:59.59 Elbow.reconfigure LooseElbow @14:53:59.63 Elbow.hold @14:53:59.68 Elbow.configure LooseElbow @14:53:59.81 SC.reconfigure SCloose @14:53:59.85 SC.hold @14:53:59.91 SC.configure SCloose @14:54:00.01 SE.reconfigure SEconfig @14:54:00.05 SE.hold @14:54:00.10 SE.configure SEconfig @14:54:00.15 CH.coast @14:54:00.20 CH.configure CHconfig @14:54:00.23 PH.coast @14:54:00.29 PH.configure PHconfig @14:54:00.33 PCR.off @14:54:00.36 PCR.pidtemp= 50,0.1,0,300,25,25 @14:54:00.39 PCR.caltemp= 2.8814,0.0372,0 @14:54:00.42 All dwarf controllers running firmware version 3.75 @14:54:00.56 Loading /home/esp/esp2/mission/20invasivedeployfinal.rb mission @14:54:00.67 GoodNews.email "Starting 20invasivedeployfinal w/pucks in tube 2\n{}\nCan@14:53:55, 17.7C, 32% humidity, 14.7psia, 15.195V, 0.594A, 0.594A avg, 9.03W",Subject: "20invasivedeployfinal begun" @14:54:00.78
Preparing to sleep until +0:00:05 @14:54:00.89 SS.coast @14:54:00.95
Asleep til +0:00:05 @14:54:01.03 Continuing at 14:54 @14:54:01.11 CC.coast @14:54:01.27 CS.coast @14:54:01.34 PC.coast @14:54:01.41 Forearm.coast @14:54:01.48 Elbow.coast @14:54:01.55 SC.coast @14:54:01.62 SE.coast @14:54:01.83 Session Begun @14:54:02.16 Gate.power :core,:OFF @14:54:02.18 Gate.power :raw,:OFF @14:54:02.21 Shutdown @14:54:05.60
Powering core bus @14:54:10.59 GoodNews.email "Then, in tube 2: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:115\n{}\nCan@14:54:01, 17.6C, 32% humidity, 14.7psia, 15.191V, 0.588A, 0.588A avg, 8.93W",Subject: "Asleep until 14:54" @14:54:10.83
Gate.power :raw,:ON @14:54:12.59 SPE.coast @14:54:12.86 SPE.configure SPEconfig @14:54:13.18 AS.configure ASconfig @14:54:13.46 SS.configure SSconfig @14:54:13.87 CC.configure CCconfig @14:54:14.23 CS.configure CSconfig @14:54:14.71 PC.configure PCconfig @14:54:15.25 PS.configure PSconfig @14:54:15.70 Forearm.configure LooseForearm @14:54:16.04 Elbow.configure LooseElbow @14:54:16.49 SC.configure SCloose @14:54:16.81 SE.configure SEconfig @14:54:16.95 CH.coast @14:54:17.30 CH.configure CHconfig @14:54:17.39 PH.coast @14:54:17.60 PH.configure PHconfig @14:54:17.73 PCR.off @14:54:17.84 PCR.pidtemp= 50,0.1,0,300,25,25 @14:54:17.94 PCR.caltemp= 2.8814,0.0372,0 @14:54:18.07 All dwarf controllers running firmware version 3.75 @14:54:18.30 Valve.reset @14:54:18.76 PCR.off @14:54:18.85 PCR.pidtemp= 50,0.1,0,300,25,25 @14:54:18.93 PCR.caltemp= 2.8814,0.0372,0 @14:54:19.29 Intake.select :close @14:54:19.56 Exhaust.select :close @14:54:19.61 CTV.dial :air @14:54:19.84 PSV.dial :air @14:54:19.96 CSV.dial :waste1 @14:54:20.61 SSV.dial :spit,avoiding: :exhaust @14:54:20.77 PS.reconfigure PSconfig @14:54:20.89
Forearm.reconfigure LooseForearm @14:54:22.71 PS.home.seek 0ml @14:54:22.88
Forearm.home.seek :retract @14:54:23.49 CS.reconfigure CSfast @14:54:23.61 SS.reconfigure SSconfig @14:54:24.04 CS.home.seek 0ml @14:54:24.34 SS.home.seek 0ml @14:54:24.64 Hand.close @14:54:24.76
Elbow.jog 1500 @14:54:25.51 Elbow.reconfigure LooseElbow @14:54:25.72 Elbow.home.seek :clear @14:54:30.69 SE.reconfigure SEconfig @14:54:30.93 SE.home.seek :down @14:54:31.75 SC.reconfigure SCsloppy @14:54:32.00 SC.home.seek :initial @14:54:32.08 PS.coast @14:54:36.28
Forearm.seek :retract @14:54:36.58 Elbow.seek :hammer @14:54:37.88 Forearm.reconfigure TightForearm @14:54:38.12 Forearm.seek 5 + 350 counts @14:54:39.44 Forearm.seek :retract @14:54:41.19 PC.reconfigure PCconfig @14:54:41.28
CC.reconfigure CCconfig @14:54:41.61 PC.home.seek :open @14:54:41.73
CC.home.seek :open @14:54:46.12 Phase [stdphase] @14:54:46.18 GoodNews.email "At 14:54:45 began in tube 2: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:115\n{}\nCan@14:54:46, 17.4C, 33% humidity, 14.7psia, 15.171V, 0.651A, 0.651A avg, 9.88W",Subject: "Started 20invasivedeployfinal phase stdphase" @14:54:46.41CEST14-May-20
===> STARTED Superphase (phase #1) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @14:54:46.47 Starting initialPurge @14:54:46.56 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:54:46.83 Hand.open @14:54:48.15 Elbow.reconfigure TightElbow @14:54:48.36 Elbow.seek FlushPuck::Garage @14:54:50.68 Elbow.reconfigure LooseElbow @14:54:51.21 Forearm.reconfigure LooseForearm @14:54:51.63 Forearm.seek :garage @14:54:52.81 Hand.close @14:54:54.03 Forearm.seek :retract @14:54:55.32 Elbow.reconfigure TightElbow @14:54:55.55 Elbow.seek CC @14:54:58.82 Elbow.reconfigure LooseElbow @14:54:59.36 Forearm.reconfigure TightForearm @14:54:59.65 Forearm.seek CC @14:55:02.33 CC.seek :closed @14:55:22.31 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.72 @14:55:22.77 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[5B:27->20] @14:55:22.88 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[5B:27->20] @14:55:23.00 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[5B:27->20] @14:55:23.09 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[5B:27->20] @14:55:23.25 Hand.open @14:55:24.46 Forearm.reconfigure LooseForearm @14:55:24.74 Forearm.seek :retract @14:55:26.00 Flushing the Collection Valve::Manifold @14:55:26.71 CSV.dial CSR @14:55:28.39 Pulling 5ml of CSR.flush @14:55:28.44 CSR.select :flush @14:55:28.89 CS.reconfigure CSconfig @14:55:29.17 CS.seek 5ml @14:55:43.13 delay 5 seconds @14:55:48.16 CSR.select :air @14:55:48.67 CS.reconfigure CSfast @14:55:48.94 CS.seek 7.5ml @14:55:55.60 CSV.dial :waste @14:55:57.07 CS.reconfigure CSconfig @14:55:57.35 CS.seek 0ml @14:56:17.67 Purging external Intake @14:56:18.38 CSV.dial :flush @14:56:19.15 CS.reconfigure CSfast @14:56:19.43 CS.seek 10ml @14:56:40.80 delay 5 seconds @14:56:45.85 CSV.dial CBV @14:56:45.92
CBV.dial CSV @14:56:46.84 CTV.dial :intake @14:56:47.66 Intake.select :open @14:56:47.86 CS.seek 0ml @14:57:10.67 Intake.select :close @14:57:10.80 CTV.dial :air @14:57:11.53 Flushing upper manifold @14:57:12.15 CSV.dial :flush @14:57:12.90 CS.seek 5ml @14:57:24.11 delay 5 seconds @14:57:29.14 CSV.dial :air @14:57:29.66 CS.seek 7ml @14:57:34.95 CSV.dial CBV @14:57:34.98
CBV.dial CSV @14:57:35.97 CTV.dial :air @14:57:36.00 CS.seek 0ml @14:57:51.42 delay 7 seconds @14:57:58.46 CS.seek 3ml @14:58:05.61 CSV.dial :waste @14:58:06.09 CS.seek 0ml @14:58:13.45 CSV.dial :flush @14:58:13.91 CS.seek 3ml @14:58:21.23 delay 5 seconds @14:58:26.26 CSV.dial :air @14:58:26.79 CS.seek 5ml @14:58:32.08 CSV.dial :air @14:58:32.10 CS.seek 0ml @14:58:43.26 delay 7 seconds @14:58:50.30 CS.seek 3ml @14:58:57.48 CSV.dial :waste @14:58:58.21 CS.seek 0ml @14:59:05.39 Garaging FlushPuck in Collection Clamp @14:59:05.49 Elbow.reconfigure TightElbow @14:59:05.56 Elbow.seek CC @14:59:05.83 Elbow.reconfigure LooseElbow @14:59:05.95 Forearm.seek CC @14:59:07.02 Hand.close @14:59:07.63 CC.seek :open @14:59:11.98 Forearm.seek :retract @14:59:13.04 Elbow.reconfigure TightElbow @14:59:13.11 Elbow.seek :garage @14:59:16.22 Elbow.reconfigure LooseElbow @14:59:16.34 Forearm.reconfigure TightForearm @14:59:16.41 Forearm.seek :garage @14:59:17.57 Hand.open @14:59:18.33 Forearm.reconfigure LooseForearm @14:59:18.39 Forearm.seek :retract @14:59:19.52 Starting mfb @14:59:19.54 pcrBAC sampling at most 1500ml, wcr at most 1500ml @14:59:19.64 BACfans.email "Started mfb in tube 2 at 14:59:19CEST14-May-20\n{}\nCan@14:54:46, 17.4C, 33% humidity, 14.7psia, 15.171V, 0.651A, 0.651A avg, 9.88W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @14:59:19.71
Loading sh1 puck from tube 2 into Collection Clamp @14:59:19.84 Readying the MFB @14:59:19.92 Homing MFB Syringe @14:59:19.94 AFV.select :holdingCoil @14:59:20.20 ATV.dial :air2 @14:59:20.34
SC.reconfigure SCloose @14:59:20.60 Elbow.reconfigure TightElbow @14:59:20.68 Elbow.seek 2 @14:59:20.82 AS.reconfigure ASconfig @14:59:20.89 AS.home.seek :home @14:59:22.80
Forearm.reconfigure TightForearm @14:59:22.87 Forearm.seek 2 + 700 counts @14:59:23.95 SE.reconfigure SEpick @14:59:24.07 SE.seek beamBreak @14:59:25.45 SE.configure SEconfig @14:59:26.02 Elbow.seek 2 @14:59:26.67 AFV.select :carrierSolution @14:59:26.87 delay 2 seconds @14:59:26.93
Forearm.seek 2 + 300 counts @14:59:27.24 Forearm.reconfigure LooseForearm @14:59:27.33 Hand.close @14:59:28.23 SE.seek :down @14:59:28.74
Forearm.seek :retract @14:59:28.94 AS.seek 1ml @14:59:29.63 SE.coast @14:59:29.86
Elbow.seek CC @14:59:31.75 Elbow.reconfigure LooseElbow @14:59:32.02 Forearm.reconfigure TightForearm @14:59:32.19 Forearm.seek CC @14:59:34.04 CC.seek :closed @14:59:38.52 Collection Clamp onSpring/offSpring for 6/32 samples, on/off torque 1.75 @14:59:38.54 Hand.open @14:59:39.44 Forearm.reconfigure LooseForearm @14:59:39.58 Forearm.seek :retract @14:59:40.70 SC.coast @14:59:40.82 collectSample 1500ml w/bubble point of 55psi @14:59:40.86 Priming 7ml @14:59:40.94 Intake.select :close @14:59:40.98 Exhaust.select :close @14:59:41.04 SS.hold @14:59:41.59 Calibrating pressure and temperature sensors @14:59:41.63 CTV.dial :air @14:59:41.69 SSV.dial :spit,avoiding: :exhaust @14:59:41.81 SS.reconfigure SSfast @14:59:41.97 SS.seek 5ml @14:59:44.91 SSV.dial CBV @14:59:44.99
CBV.dial SSV @14:59:46.33 delay 3.8 seconds @14:59:50.41 SSV.dial :spit @14:59:51.76 SS.seek 0ml @14:59:54.30 SSV.dial CBV @14:59:55.65 CTV.dial :intake @14:59:56.48 SSV.dial CBV @14:59:56.54
CBV.dial SSV @14:59:56.59 Allowing 40s for 7.0ml of remaining 7.0ml @14:59:56.65 Intake.select :open @14:59:58.99 Intake @33.3psia, vacuum from 20.0 to 24.0psi @14:59:59.06 SS.reconfigure SSinhale.with maxSpeed: 150 @14:59:59.23 SS.seek 7ml @15:00:02.11 Vacuum too high -- waiting up to 40s @15:00:03.85 Intake @36.3psia, vacuum from 20.0 to 24.0psi @15:00:03.90 SS.reconfigure SSinhale.with maxSpeed: 135 @15:00:04.06 SS.seek 7ml @15:00:06.49 Vacuum too high -- waiting up to 40s @15:00:07.86 Intake @36.7psia, vacuum from 20.0 to 24.0psi @15:00:07.91 SS.reconfigure SSinhale.with maxSpeed: 122 @15:00:08.09 SS.seek 7ml @15:00:10.39 Vacuum too high -- waiting up to 40s @15:00:11.68 Intake @37.1psia, vacuum from 20.0 to 24.0psi @15:00:11.73 SS.reconfigure SSinhale.with maxSpeed: 110 @15:00:11.90 SS.seek 7ml @15:00:14.20 Vacuum too high -- waiting up to 40s @15:00:16.49 Intake @36.7psia, vacuum from 20.0 to 24.0psi @15:00:16.55 SS.reconfigure SSinhale.with maxSpeed: 99 @15:00:16.73 SS.seek 7ml @15:00:17.36 Stroke completed -- waiting for pressure to equalize ... @15:00:18.26 Intake.select :close @15:00:18.55 SSV.dial :exhaust @15:00:19.36 Exhaust.select :open @15:00:19.56 SS.reconfigure SSexhale @15:00:19.70 SS.seek 0ml @15:00:21.92 delay 1 second @15:00:22.95 AFV.select :holdingCoil @15:00:23.12 delay 1 second @15:00:23.58
Exhaust.select :close @15:00:23.78 Sampling 1500.0ml @15:00:23.81 Intake.select :close @15:00:23.86 Exhaust.select :close @15:00:23.92 SS.reconfigure SSconfig @15:00:24.05 SS.hold @15:00:24.36 ATV.dial :waste @15:00:24.74
CTV.dial :intake @15:00:24.79 SSV.dial CBV @15:00:24.87
CBV.dial SSV @15:00:25.50 AS.reconfigure ASnorm @15:00:25.64 AS.home.seek :empty @15:00:25.83
Allowing 130s for 25.0ml of remaining 1500.0ml @15:00:25.86 Intake.select :open @15:00:28.17 Intake @32.0psia, vacuum from 20.0 to 24.0psi @15:00:28.23 SS.reconfigure SSinhale.with maxSpeed: 99 @15:00:28.30 SS.seek 25ml @15:00:31.29 Vacuum too high -- waiting up to 40s @15:00:33.36 Intake @36.3psia, vacuum from 20.0 to 24.0psi @15:00:33.38 SS.reconfigure SSinhale.with maxSpeed: 89 @15:00:33.47 SS.seek 25ml @15:00:35.53 Vacuum too high -- waiting up to 40s @15:00:36.62 Intake @36.4psia, vacuum from 20.0 to 24.0psi @15:00:36.64 SS.reconfigure SSinhale.with maxSpeed: 80 @15:00:36.73 SS.seek 25ml @15:00:38.57 Vacuum too high -- waiting up to 40s @15:00:39.64 SS.reconfigure SSinhale.with maxSpeed: 72 @15:00:39.71 SS.seek 25ml @15:00:41.76 Vacuum too high -- waiting up to 40s @15:00:42.83 Intake @36.3psia, vacuum from 20.0 to 24.0psi @15:00:42.86 SS.reconfigure SSinhale.with maxSpeed: 65 @15:00:42.95 SS.seek 25ml @15:00:45.07 Vacuum too high -- waiting up to 40s @15:00:46.14 SS.reconfigure SSinhale.with maxSpeed: 59 @15:00:46.21 SS.seek 25ml @15:00:48.41 Vacuum too high -- waiting up to 40s @15:00:49.47 Intake @36.2psia, vacuum from 20.0 to 24.0psi @15:00:49.49 SS.reconfigure SSinhale.with maxSpeed: 53 @15:00:49.58 SS.seek 25ml @15:00:51.97 Vacuum too high -- waiting up to 40s @15:00:53.03 Intake @36.1psia, vacuum from 20.0 to 24.0psi @15:00:53.06 SS.reconfigure SSinhale.with maxSpeed: 48 @15:00:53.14 SS.seek 25ml @15:00:55.97 Vacuum too high -- waiting up to 40s @15:00:56.81 SS.reconfigure SSinhale.with maxSpeed: 43 @15:00:56.88 SS.seek 25ml @15:00:59.97 Vacuum too high -- waiting up to 40s @15:01:00.80 Intake @35.9psia, vacuum from 20.0 to 24.0psi @15:01:00.83 SS.reconfigure SSinhale.with maxSpeed: 39 @15:01:00.91 SS.seek 25ml @15:01:05.31 Vacuum too high -- waiting up to 40s @15:01:06.16 Intake @35.8psia, vacuum from 20.0 to 24.0psi @15:01:06.19 SS.reconfigure SSinhale.with maxSpeed: 35 @15:01:06.27 SS.seek 25ml @15:01:15.51 Vacuum too high -- waiting up to 40s @15:01:16.33 Intake @35.6psia, vacuum from 20.0 to 24.0psi @15:01:16.35 SS.reconfigure SSinhale.with maxSpeed: 32 @15:01:16.44 SS.seek 25ml @15:01:18.25 ATV.dial :waste @15:01:18.26 ARV.dial between carrier and air1 @15:01:18.37 ASV.dial :waste1 @15:01:26.91
Stroke completed -- waiting for pressure to equalize ... @15:01:30.73 Intake.select :close @15:01:30.91 SSV.dial :exhaust @15:01:31.66 Exhaust.select :open @15:01:31.80 SS.reconfigure SSexhale @15:01:31.86 SS.seek 0ml @15:01:41.17 Exhaust.select :close @15:01:41.31 SSV.dial CBV @15:01:41.34
CBV.dial SSV @15:01:42.12 Allowing 130s for 25.0ml of remaining 1475.0ml @15:01:42.14 Intake.select :open @15:01:44.35 Intake @32.2psia, vacuum from 20.0 to 24.0psi @15:01:44.37 SS.reconfigure SSinhale.with maxSpeed: 32 @15:01:44.46 SS.seek 25ml @15:02:39.28 Stroke completed -- waiting for pressure to equalize ... @15:02:43.07 Intake.select :close @15:02:43.25 SSV.dial :exhaust @15:02:44.00 Exhaust.select :open @15:02:44.14 SS.reconfigure SSexhale @15:02:44.20 SS.seek 0ml @15:02:52.86 Exhaust.select :close @15:02:53.00 SSV.dial CBV @15:02:53.04
CBV.dial SSV @15:02:53.77 Allowing 130s for 25.0ml of remaining 1450.0ml @15:02:53.79 Intake.select :open @15:02:55.99 Intake @32.1psia, vacuum from 20.0 to 24.0psi @15:02:56.01 SS.reconfigure SSinhale.with maxSpeed: 32 @15:02:56.10 SS.seek 25ml @15:03:51.21 Stroke completed -- waiting for pressure to equalize ... @15:03:55.02 Intake.select :close @15:03:55.20 SSV.dial :exhaust @15:03:55.94 Exhaust.select :open @15:03:56.08 SS.reconfigure SSexhale @15:03:56.14 SS.seek 0ml @15:04:05.06 Exhaust.select :close @15:04:05.20 SSV.dial CBV @15:04:05.23
CBV.dial SSV @15:04:05.98 Allowing 130s for 25.0ml of remaining 1425.0ml @15:04:06.00 Intake.select :open @15:04:08.20 Intake @32.0psia, vacuum from 20.0 to 24.0psi @15:04:08.22 SS.reconfigure SSinhale.with maxSpeed: 32 @15:04:08.31 SS.seek 25ml @15:05:03.42 Stroke completed -- waiting for pressure to equalize ... @15:05:07.12 Intake.select :close @15:05:07.30 SSV.dial :exhaust @15:05:08.05 Exhaust.select :open @15:05:08.19 SS.reconfigure SSexhale @15:05:08.26 SS.seek 0ml @15:05:17.20 Exhaust.select :close @15:05:17.34 SSV.dial CBV @15:05:17.37
CBV.dial SSV @15:05:18.14 Allowing 130s for 25.0ml of remaining 1400.0ml @15:05:18.16 Intake.select :open @15:05:20.36 Intake @31.8psia, vacuum from 20.0 to 24.0psi @15:05:20.39 SS.reconfigure SSinhale.with maxSpeed: 32 @15:05:20.47 SS.seek 25ml @15:06:15.25 Stroke completed -- waiting for pressure to equalize ... @15:06:19.07 Intake.select :close @15:06:19.24 SSV.dial :exhaust @15:06:20.00 Exhaust.select :open @15:06:20.14 SS.reconfigure SSexhale @15:06:20.21 SS.seek 0ml @15:06:29.00 Exhaust.select :close @15:06:29.14 SSV.dial CBV @15:06:29.17
CBV.dial SSV @15:06:29.95 Allowing 130s for 25.0ml of remaining 1375.0ml @15:06:29.97 Intake.select :open @15:06:32.17 Intake @31.8psia, vacuum from 20.0 to 24.0psi @15:06:32.19 SS.reconfigure SSinhale.with maxSpeed: 32 @15:06:32.28 SS.seek 25ml @15:07:27.35 Stroke completed -- waiting for pressure to equalize ... @15:07:31.79 Intake.select :close @15:07:31.96 SSV.dial :exhaust @15:07:32.72 Exhaust.select :open @15:07:32.86 SS.reconfigure SSexhale @15:07:32.93 SS.seek 0ml @15:07:41.65 Exhaust.select :close @15:07:41.79 SSV.dial CBV @15:07:41.82
CBV.dial SSV @15:07:42.59 Allowing 130s for 25.0ml of remaining 1350.0ml @15:07:42.61 Intake.select :open @15:07:44.81 Intake @31.7psia, vacuum from 20.0 to 24.0psi @15:07:44.84 SS.reconfigure SSinhale.with maxSpeed: 32 @15:07:44.92 SS.seek 25ml @15:08:40.05 Stroke completed -- waiting for pressure to equalize ... @15:08:45.12 Intake.select :close @15:08:45.29 SSV.dial :exhaust @15:08:46.05 Exhaust.select :open @15:08:46.19 SS.reconfigure SSexhale @15:08:46.25 SS.seek 0ml @15:08:54.95 Exhaust.select :close @15:08:55.09 SSV.dial CBV @15:08:55.12
CBV.dial SSV @15:08:55.89 Allowing 130s for 25.0ml of remaining 1325.0ml @15:08:55.91 Intake.select :open @15:08:58.11 Intake @31.7psia, vacuum from 20.0 to 24.0psi @15:08:58.13 SS.reconfigure SSinhale.with maxSpeed: 32 @15:08:58.22 SS.seek 25ml @15:09:49.15 Vacuum too high -- waiting up to 40s @15:09:51.49 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:09:51.51 SS.reconfigure SSinhale.with maxSpeed: 29 @15:09:51.59 SS.seek 25ml @15:09:56.84 Stroke completed -- waiting for pressure to equalize ... @15:10:00.70 Intake.select :close @15:10:00.89 SSV.dial :exhaust @15:10:01.65 Exhaust.select :open @15:10:01.79 SS.reconfigure SSexhale @15:10:02.00 SS.seek 0ml @15:10:10.83 Exhaust.select :close @15:10:10.96 SSV.dial CBV @15:10:10.99
CBV.dial SSV @15:10:11.78 Allowing 130s for 25.0ml of remaining 1300.0ml @15:10:11.80 Intake.select :open @15:10:14.00 Intake @31.7psia, vacuum from 20.0 to 24.0psi @15:10:14.03 SS.reconfigure SSinhale.with maxSpeed: 29 @15:10:14.11 SS.seek 25ml @15:10:26.78 Session Ended @15:11:14.80
Stroke completed -- waiting for pressure to equalize ... @15:11:20.49 Intake.select :close @15:11:20.65 SSV.dial :exhaust @15:11:21.39 Exhaust.select :open @15:11:21.51 SS.reconfigure SSexhale @15:11:21.56 SS.seek 0ml @15:11:30.83 Exhaust.select :close @15:11:30.95 SSV.dial CBV @15:11:30.98
CBV.dial SSV @15:11:31.73 Allowing 130s for 25.0ml of remaining 1275.0ml @15:11:31.74 Intake.select :open @15:11:33.91 Intake @31.8psia, vacuum from 20.0 to 24.0psi @15:11:33.93 SS.reconfigure SSinhale.with maxSpeed: 29 @15:11:33.98 SS.seek 25ml @15:12:34.41 Stroke completed -- waiting for pressure to equalize ... @15:12:40.73 Intake.select :close @15:12:40.89 SSV.dial :exhaust @15:12:41.62 Exhaust.select :open @15:12:41.74 SS.reconfigure SSexhale @15:12:41.78 SS.seek 0ml @15:12:50.50 Exhaust.select :close @15:12:50.62 SSV.dial CBV @15:12:50.65
CBV.dial SSV @15:12:51.42 Allowing 130s for 25.0ml of remaining 1250.0ml @15:12:51.43 Intake.select :open @15:12:53.60 Intake @31.8psia, vacuum from 20.0 to 24.0psi @15:12:53.62 SS.reconfigure SSinhale.with maxSpeed: 29 @15:12:53.67 SS.seek 25ml @15:13:35.41 Vacuum too high -- waiting up to 40s @15:13:37.97 Intake @33.3psia, vacuum from 20.0 to 24.0psi @15:13:38.36 SS.reconfigure SSinhale.with maxSpeed: 26 @15:13:38.54 SS.seek 25ml @15:14:00.21 Stroke completed -- waiting for pressure to equalize ... @15:14:06.55 Intake.select :close @15:14:06.71 SSV.dial :exhaust @15:14:07.45 Exhaust.select :open @15:14:07.57 SS.reconfigure SSexhale @15:14:07.61 SS.seek 0ml @15:14:16.54 Exhaust.select :close @15:14:16.65 SSV.dial CBV @15:14:16.68
CBV.dial SSV @15:14:17.44 Allowing 130s for 25.0ml of remaining 1225.0ml @15:14:17.45 Intake.select :open @15:14:19.62 Intake @31.6psia, vacuum from 20.0 to 24.0psi @15:14:19.64 SS.reconfigure SSinhale.with maxSpeed: 26 @15:14:19.69 SS.seek 25ml @15:15:26.95 Stroke completed -- waiting for pressure to equalize ... @15:15:34.66 Intake.select :close @15:15:34.82 SSV.dial :exhaust @15:15:35.56 Exhaust.select :open @15:15:35.68 SS.reconfigure SSexhale @15:15:35.72 SS.seek 0ml @15:15:44.63 Exhaust.select :close @15:15:44.76 SSV.dial CBV @15:15:44.78
CBV.dial SSV @15:15:45.54 Allowing 130s for 25.0ml of remaining 1200.0ml @15:15:45.55 Intake.select :open @15:15:47.72 Intake @31.7psia, vacuum from 20.0 to 24.0psi @15:15:47.74 SS.reconfigure SSinhale.with maxSpeed: 26 @15:15:47.79 SS.seek 25ml @15:16:27.97 Vacuum too high -- waiting up to 40s @15:16:31.03 Intake @33.1psia, vacuum from 20.0 to 24.0psi @15:16:31.05 SS.reconfigure SSinhale.with maxSpeed: 23 @15:16:31.10 SS.seek 25ml @15:17:01.07 Vacuum too high -- waiting up to 40s @15:17:03.23 Intake @33.0psia, vacuum from 20.0 to 24.0psi @15:17:03.26 SS.reconfigure SSinhale.with maxSpeed: 21 @15:17:03.37 SS.seek 25ml @15:17:03.71 Session Begun @15:17:05.06
Stroke completed -- waiting for pressure to equalize ... @15:17:09.54 Intake.select :close @15:17:09.73 SSV.dial :exhaust @15:17:10.48 Exhaust.select :open @15:17:10.63 SS.reconfigure SSexhale @15:17:10.70 SS.seek 0ml @15:17:19.64 Exhaust.select :close @15:17:19.78 SSV.dial CBV @15:17:19.81
CBV.dial SSV @15:17:20.59 Allowing 130s for 25.0ml of remaining 1175.0ml @15:17:20.61 Intake.select :open @15:17:22.81 Intake @31.8psia, vacuum from 20.0 to 24.0psi @15:17:22.84 SS.reconfigure SSinhale.with maxSpeed: 21 @15:17:22.93 SS.seek 25ml @15:18:46.50 Stroke completed -- waiting for pressure to equalize ... @15:18:54.08 Intake.select :close @15:18:54.27 SSV.dial :exhaust @15:18:55.03 Exhaust.select :open @15:18:55.17 SS.reconfigure SSexhale @15:18:55.24 SS.seek 0ml @15:19:04.09 Exhaust.select :close @15:19:04.23 SSV.dial CBV @15:19:04.26
CBV.dial SSV @15:19:05.04 Allowing 130s for 25.0ml of remaining 1150.0ml @15:19:05.06 Intake.select :open @15:19:07.28 Intake @31.7psia, vacuum from 20.0 to 24.0psi @15:19:07.30 SS.reconfigure SSinhale.with maxSpeed: 21 @15:19:07.38 SS.seek 25ml @15:20:13.82 Vacuum too high -- waiting up to 40s @15:20:16.63 Intake @32.8psia, vacuum from 20.0 to 24.0psi @15:20:16.65 SS.reconfigure SSinhale.with maxSpeed: 19 @15:20:16.77 SS.seek 25ml @15:20:35.78 Stroke completed -- waiting for pressure to equalize ... @15:20:43.85 Intake.select :close @15:20:44.03 SSV.dial :exhaust @15:20:44.79 Exhaust.select :open @15:20:44.93 SS.reconfigure SSexhale @15:20:45.00 SS.seek 0ml @15:20:53.83 Exhaust.select :close @15:20:53.97 SSV.dial CBV @15:20:54.00
CBV.dial SSV @15:20:54.78 Allowing 130s for 25.0ml of remaining 1125.0ml @15:20:54.80 Intake.select :open @15:20:57.00 Intake @31.8psia, vacuum from 20.0 to 24.0psi @15:20:57.03 SS.reconfigure SSinhale.with maxSpeed: 19 @15:20:57.12 SS.seek 25ml @15:22:29.63 Stroke completed -- waiting for pressure to equalize ... @15:22:39.67 Intake.select :close @15:22:39.85 SSV.dial :exhaust @15:22:40.59 Exhaust.select :open @15:22:40.73 SS.reconfigure SSexhale @15:22:40.80 SS.seek 0ml @15:22:49.72 Exhaust.select :close @15:22:49.86 SSV.dial CBV @15:22:49.89
CBV.dial SSV @15:22:50.68 Allowing 130s for 25.0ml of remaining 1100.0ml @15:22:50.69 Intake.select :open @15:22:52.90 Intake @31.8psia, vacuum from 20.0 to 24.0psi @15:22:52.92 SS.reconfigure SSinhale.with maxSpeed: 19 @15:22:53.01 SS.seek 25ml @15:23:40.44 Vacuum too high -- waiting up to 40s @15:23:44.25 Intake @32.7psia, vacuum from 20.0 to 24.0psi @15:23:44.27 SS.reconfigure SSinhale.with maxSpeed: 17 @15:23:44.36 SS.seek 25ml @15:24:23.65 Vacuum too high -- waiting up to 40s @15:24:26.75 Intake @32.7psia, vacuum from 20.0 to 24.0psi @15:24:26.78 SS.reconfigure SSinhale.with maxSpeed: 15 @15:24:26.87 SS.seek 25ml @15:24:40.95 Stroke completed -- waiting for pressure to equalize ... @15:24:49.12 Intake.select :close @15:24:49.29 SSV.dial :exhaust @15:24:50.06 Exhaust.select :open @15:24:50.20 SS.reconfigure SSexhale @15:24:50.26 SS.seek 0ml @15:24:59.15 Exhaust.select :close @15:24:59.29 SSV.dial CBV @15:24:59.32
CBV.dial SSV @15:25:00.12 Allowing 130s for 25.0ml of remaining 1075.0ml @15:25:00.13 Intake.select :open @15:25:02.34 Intake @31.8psia, vacuum from 20.0 to 24.0psi @15:25:02.36 SS.reconfigure SSinhale.with maxSpeed: 15 @15:25:02.45 SS.seek 25ml @15:26:59.81 Stroke completed -- waiting for pressure to equalize ... @15:27:10.14 SS.reconfigure SSconfig @15:27:10.21 SS.hold @15:27:10.36 Intake.select :close @15:27:10.53 ShallowSampler::Clogged in MAIN -- progress too slow after 450.0ml @15:27:10.55 SSV.dial :exhaust @15:27:11.33 Exhaust.select :open @15:27:11.48 SS.reconfigure SSexhale @15:27:11.54 SS.seek 0ml @15:27:20.42 Exhaust.select :close @15:27:20.55 Sampled 450.0ml @15:27:20.57 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @15:27:20.60 Intake.select :close @15:27:20.70 CSV.dial :waste @15:27:20.79 CS.seek 0ml @15:27:20.90 CSV.dial :air @15:27:21.44 SSV.dial CBV,avoiding: :exhaust @15:27:21.50
CBV.dial SSV @15:27:22.22 CS.seek 10ml @15:27:23.33
CTV.dial :air @15:27:24.08 delay 15 seconds @15:27:39.11 SSV.dial :spit,avoiding: :exhaust @15:27:40.38 CTV.dial CSV,avoiding: :intake @15:27:40.42
SS.seek 0ml @15:27:40.58 SSV.dial CBV,avoiding: :exhaust @15:27:44.03 CBV.dial between SSV and waste2 @15:27:44.47 CTV.dial CSV,avoiding: :air @15:27:44.51
CSV.dial CTV @15:27:45.11 CS.reconfigure CSpressurize @15:27:45.19 CS.seek 0ml @15:28:11.21 BubblePoint: Intake @72.1psia, Outlet @16.5psia @15:28:11.22 CBV.dial SSV @15:28:11.60 SS.reconfigure SSconfig @15:28:11.68 SS.seek 10ml @15:28:17.17 delay 15 seconds @15:28:32.32 Evacuated: Intake @37.0psia, Outlet @2.7psia @15:28:32.33 SSV.dial :spit,avoiding: :exhaust @15:28:33.62 SS.reconfigure SSexhale @15:28:33.70 SS.seek 0ml @15:28:38.57 SS.reconfigure SSfast @15:28:38.65 SS.seek 5ml @15:28:41.48 SSV.dial CBV,avoiding: :exhaust @15:28:41.52
CBV.dial SSV @15:28:42.81 CTV.dial :air,avoiding: :intake @15:28:44.10 CSV.dial CTV @15:28:44.15
SS.seek 10ml @15:28:45.15 CSV.dial :air @15:28:45.61 CS.reconfigure CSfast @15:28:45.68 CS.seek 0ml @15:28:46.41 CS.coast @15:28:46.73
SSV.dial :spit,avoiding: :exhaust @15:28:48.01 SS.reconfigure SSexhale @15:28:48.09 SS.seek 0ml @15:28:52.91 SS.reconfigure SSfast @15:28:52.99 SS.seek 5ml @15:28:55.64 SSV.dial CBV,avoiding: :exhaust @15:28:55.68
CBV.dial SSV @15:28:57.98 SSV.dial :spit,avoiding: :exhaust @15:28:59.26 SS.reconfigure SSconfig @15:28:59.34 SS.seek 0ml @15:29:02.44 SS.coast @15:29:02.51 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @15:29:02.52 Intake.select :close @15:29:02.64 CSV.dial :waste @15:29:02.78 SSV.dial CBV,avoiding: :exhaust @15:29:02.81
CBV.dial SSV @15:29:03.43 CS.seek 0ml @15:29:03.54 CSV.dial :air @15:29:04.11
CTV.dial :air @15:29:04.13 delay 15 seconds @15:29:04.28 CS.seek 10ml @15:29:19.17
SSV.dial :spit,avoiding: :exhaust @15:29:20.46 CTV.dial CSV,avoiding: :intake @15:29:20.50
SS.reconfigure SSexhale @15:29:20.63 SS.seek 0ml @15:29:20.71 SSV.dial CBV,avoiding: :exhaust @15:29:25.94 CBV.dial between SSV and waste2 @15:29:26.86 CSV.dial between air and CTV @15:29:27.20 CS.seek 8.16ml @15:29:32.33 CTV.dial CSV,avoiding: :air @15:29:32.36
CSV.dial CTV @15:29:32.72 CS.reconfigure CSpressurize @15:29:32.79 CS.seek 0ml @15:29:54.32 CSV.dial :air @15:29:54.80 CS.reconfigure CSfast @15:29:54.87 CS.seek 10ml @15:30:16.07 CSV.dial between air and CTV @15:30:16.44 CS.seek 3.081ml @15:30:31.49 CTV.dial CSV,avoiding: :air @15:30:31.52
CSV.dial CTV @15:30:31.88 CS.reconfigure CSpressurize @15:30:31.95 CS.seek 0ml @15:30:39.85 BubblePoint: Intake @71.8psia, Outlet @16.5psia @15:30:39.86 CBV.dial SSV @15:30:40.23 SS.reconfigure SSconfig @15:30:40.31 SS.seek 10ml @15:30:45.70 delay 15 seconds @15:31:00.84 Evacuated: Intake @39.1psia, Outlet @18.5psia @15:31:00.86 SSV.dial :spit,avoiding: :exhaust @15:31:02.15 SS.reconfigure SSexhale @15:31:02.22 SS.seek 0ml @15:31:07.04 SS.reconfigure SSfast @15:31:07.12 SS.seek 5ml @15:31:09.76 SSV.dial CBV,avoiding: :exhaust @15:31:09.80
CBV.dial SSV @15:31:11.09 CTV.dial :air,avoiding: :intake @15:31:12.40 CSV.dial CTV @15:31:12.45
SS.seek 10ml @15:31:13.44 CSV.dial :air @15:31:13.90 CS.reconfigure CSfast @15:31:13.98 CS.seek 0ml @15:31:14.90
SSV.dial :spit,avoiding: :exhaust @15:31:15.60 CS.coast @15:31:16.18
SS.reconfigure SSexhale @15:31:16.25 SS.seek 0ml @15:31:21.30 SS.reconfigure SSfast @15:31:21.38 SS.seek 5ml @15:31:24.07 SSV.dial CBV,avoiding: :exhaust @15:31:24.10
CBV.dial SSV @15:31:26.41 SSV.dial :spit,avoiding: :exhaust @15:31:27.70 SS.reconfigure SSconfig @15:31:27.77 SS.seek 0ml @15:31:30.98 SS.coast @15:31:31.05 Lysing: Adding 1.5ml lysis @15:31:31.06 CBV.dial between CSV and waste1,avoiding: :DBV @15:31:32.49 CTV.dial CSV @15:31:33.76 CSV.dial CSR @15:31:34.54 Pulling 1ml of CSR.lysis @15:31:34.55 CSR.select :lysis @15:31:34.71 CS.reconfigure CSconfig @15:31:34.78 CS.seek 1ml @15:31:38.54 delay 5 seconds @15:31:43.57 CSR.select :air @15:31:43.77 CS.seek 6ml @15:31:57.48 delay 2 seconds @15:31:59.50 CSV.dial :waste1 @15:32:00.74 CS.reconfigure CSfast @15:32:01.31 CS.seek :empty @15:32:14.48 CSV.dial CSR @15:32:15.90 Pulling 1.5ml of CSR.lysis @15:32:15.92 CSR.select :lysis @15:32:16.08 CS.reconfigure CSconfig @15:32:16.15 CS.seek 1.5ml @15:32:21.01 delay 5 seconds @15:32:26.04 CSR.select :air @15:32:26.25 CS.seek 4.5ml @15:32:34.85 delay 2 seconds @15:32:36.87 CSV.dial CTV @15:32:37.35 CS.seek 1.275ml @15:32:46.52 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @15:32:46.54 CH.seek 85C,8:00 @15:35:13.81 CH.hold 5:00,85C @15:40:14.00 Collection Puck Heater at 85.1C after lysing @15:40:14.03 CS.seek 0ml @15:40:18.38 CTV.dial between spare and air,avoiding: :intake @15:40:19.40 CSV.dial :air @15:40:19.88 CS.seek 5ml @15:40:33.67 CSV.dial CTV @15:40:34.14 delay 2 seconds @15:40:36.16 CSV.dial :air @15:40:36.62 delay 2 seconds @15:40:38.64 CSV.dial CTV @15:40:39.12 delay 2 seconds @15:40:41.15 CSV.dial :air @15:40:41.61 delay 2 seconds @15:40:43.64 CS.seek 0ml @15:40:57.45 Recovering Lysate into Collection syringe @15:40:57.46 CSV.dial CBV @15:40:58.47 CBV.dial CSV @15:40:58.88 CS.seek 5ml @15:41:12.67 delay 5 seconds @15:41:17.70 CTV.dial :air @15:41:18.24 CS.seek 7.001ml @15:41:24.45 delay 5 seconds @15:41:29.48 CSV.dial :air2 @15:41:29.95 CS.seek 10ml @15:41:38.61 CSV.dial between air and CTV,avoiding: CBV @15:41:39.70 Lysing Complete @15:41:39.81 Storing sh1 puck in Collection Clamp to tube 1 @15:41:40.01 SC.reconfigure SCtight @15:41:40.08 SC.seek 1 @15:41:42.22 SC.reconfigure SCloose @15:41:42.47 Elbow.reconfigure TightElbow @15:41:42.54 Elbow.seek 1 @15:41:44.54 Forearm.reconfigure TightForearm @15:41:44.61 Forearm.seek 1 + 700 counts @15:41:46.20 SE.reconfigure SEpick @15:41:46.31 SE.seek beamBreak @15:41:49.11 SE.configure SEconfig @15:41:49.31 Elbow.reconfigure LimpElbow @15:41:49.38 Forearm.reconfigure LooseForearm @15:41:49.46 Forearm.seek 1 @15:41:49.81 Elbow.hold @15:41:49.90 Elbow.reconfigure TightElbow @15:41:50.03 SE.seek 2.07 Pucks below Top Plate @15:41:50.54 Forearm.force -20 @15:41:50.60 Forearm.seek 1 + 304 counts @15:41:50.97 Forearm.reconfigure TightForearm @15:41:51.04 Forearm.seek 1 + 700 counts @15:41:51.39 SE.seek 3.07 Pucks below Top Plate @15:41:51.97 Forearm.reconfigure LooseForearm @15:41:52.04 Forearm.seek :retract @15:41:52.97 Elbow.seek CC @15:41:54.67 Elbow.reconfigure LooseElbow @15:41:54.80 Forearm.seek CC @15:41:55.89 Hand.close @15:41:56.56 CC.seek :open @15:42:01.05 Forearm.seek :retract @15:42:02.11 Elbow.reconfigure TightElbow @15:42:02.19 Elbow.seek 1 @15:42:04.24 Elbow.reconfigure LimpElbow @15:42:04.32 Forearm.seek 1 @15:42:05.33 Elbow.hold @15:42:05.43 Elbow.reconfigure TightElbow @15:42:05.50 Hand.open @15:42:06.33 Forearm.force -20 @15:42:06.40 Forearm.seek 1 + 350 counts @15:42:06.73 Forearm.reconfigure TightForearm @15:42:06.81 Forearm.seek 1 + 700 counts @15:42:07.16 SE.seek :down @15:42:08.31
Forearm.reconfigure LooseForearm @15:42:08.38 Forearm.seek :retract @15:42:09.78 SE.coast @15:42:09.84
SC.coast @15:42:09.91 Tranferring lysate to PRV @15:42:09.93 CSV.dial :air @15:42:10.28 CS.seek 10ml @15:42:10.39 CSV.dial between air and CTV @15:42:10.72 AIV.dial :bypass @15:42:10.83 Beginning samplePassC @15:42:10.85 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:42:10.95 Hand.open @15:42:11.34 Elbow.seek FlushPuck::Garage @15:42:13.79 Elbow.reconfigure LooseElbow @15:42:13.91 Forearm.seek :garage @15:42:14.97 Hand.close @15:42:15.70 Forearm.seek :retract @15:42:16.74 Elbow.reconfigure TightElbow @15:42:16.81 Elbow.seek CC @15:42:19.73 Elbow.reconfigure LooseElbow @15:42:19.87 Forearm.reconfigure TightForearm @15:42:19.95 Forearm.seek CC @15:42:21.29 CC.seek :closed @15:42:25.59 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.77 @15:42:25.71 Hand.open @15:42:26.38 Forearm.reconfigure LooseForearm @15:42:26.45 Forearm.seek :retract @15:42:27.52 AWV.select :air @15:42:27.53 AIV.dial :bypass @15:42:27.57 PSV.dial PSR @15:42:28.87 Pulling 1.5ml of PSR.flush @15:42:28.89 PSR.select :flush @15:42:29.06 PS.seek 1.5ml @15:42:45.06 delay 5 seconds @15:42:50.09 PSV.dial :waste1 @15:42:50.68 PS.seek 0ml @15:43:06.72 PSV.dial PSR @15:43:07.29 PSR.select :air @15:43:07.44 PS.seek 2.5ml @15:43:33.54 PRV.dial CBV @15:43:33.57
CBV.dial PRV @15:43:34.38 PSV.dial :mixloop @15:43:35.36 CTV.dial :air,avoiding: :intake @15:43:35.38 PTV.dial :PRVmixing @15:43:35.51 PS.seek 0ml @15:44:01.72 PSV.dial :air @15:44:02.20 PS.seek 2.5ml @15:44:28.44 PSV.dial :mixloop @15:44:28.92 PS.seek 0ml @15:44:55.12 PSV.dial :air @15:44:55.60 PS.seek 2.5ml @15:45:21.75 PSV.dial :mixloop @15:45:22.21 PS.seek 0ml @15:45:48.41 Garaging FlushPuck in Collection Clamp @15:45:48.52 Elbow.reconfigure TightElbow @15:45:48.58 Elbow.seek CC @15:45:48.85 Elbow.reconfigure LooseElbow @15:45:48.98 Forearm.seek CC @15:45:50.04 Hand.close @15:45:50.69 CC.seek :open @15:45:55.06 Forearm.seek :retract @15:45:56.09 Elbow.reconfigure TightElbow @15:45:56.17 Elbow.seek :garage @15:45:59.37 Elbow.reconfigure LooseElbow @15:45:59.50 Forearm.reconfigure TightForearm @15:45:59.58 Forearm.seek :garage @15:46:00.74 Hand.open @15:46:01.52 Forearm.reconfigure LooseForearm @15:46:01.59 Forearm.seek :retract @15:46:02.66 Connecting PS to CS to transfer sample @15:46:02.67 CBV.dial AIV @15:46:02.70 AIV.dial :bypass @15:46:02.73 PSV.dial :mixloop @15:46:02.76 PTV.dial :PRVmixing @15:46:02.79 PRV.dial AIV @15:46:02.82 CTV.dial :air,avoiding: :intake @15:46:02.85 Loading lyfil puck from tube 2 into Collection Clamp @15:46:03.15 SC.reconfigure SCtight @15:46:03.22 SC.seek 2 @15:46:05.35 SC.reconfigure SCloose @15:46:05.61 Elbow.reconfigure TightElbow @15:46:05.68 Elbow.seek 2 @15:46:07.83 Forearm.reconfigure TightForearm @15:46:07.91 Forearm.seek 2 + 700 counts @15:46:08.98 SE.reconfigure SEpick @15:46:09.08 SE.seek beamBreak @15:46:10.49 SE.configure SEconfig @15:46:10.69 Elbow.seek 2 @15:46:10.92 Forearm.seek 2 + 300 counts @15:46:11.15 Forearm.reconfigure LooseForearm @15:46:11.22 Hand.close @15:46:11.84 SE.seek :down @15:46:12.06
Forearm.seek :retract @15:46:12.96 SE.coast @15:46:13.05
Elbow.seek CC @15:46:14.72 Elbow.reconfigure LooseElbow @15:46:14.86 Forearm.reconfigure TightForearm @15:46:14.93 Forearm.seek CC @15:46:16.33 CC.seek :closed @15:46:20.62 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.77 @15:46:20.64 Hand.open @15:46:21.40 Forearm.reconfigure LooseForearm @15:46:21.47 Forearm.seek :retract @15:46:22.57 SC.coast @15:46:22.63 CTV.dial CSV,avoiding: :intake @15:46:23.91 CSV.dial CTV @15:46:24.25 CS.seek 5ml @15:46:37.96 delay 1:20 @15:47:58.00 CS.seek 2.5ml @15:48:05.43 delay 40 seconds @15:48:45.47 CS.seek 0ml @15:48:53.02 delay 1:00 @15:49:53.05 PSV.dial :air @15:49:53.53 PS.seek 2.5ml @15:50:19.69 PSV.dial :mixloop @15:50:20.16 delay 3 seconds @15:50:23.19 PSV.dial :air @15:50:23.64 delay 2 seconds @15:50:25.66 PSV.dial :mixloop @15:50:26.66 delay 3 seconds @15:50:29.69 PSV.dial :waste1 @15:50:30.93 PS.seek :empty @15:50:57.07 CBV.dial :waste1 @15:50:57.56 delay 5 seconds @15:51:02.59 CBV.dial AIV @15:51:03.07 delay 10 seconds @15:51:13.10 CBV.dial :waste1 @15:51:13.57 delay 10 seconds @15:51:23.60 CBV.dial AIV @15:51:24.07 delay 10 seconds @15:51:34.10 CTV.dial CSV,avoiding: :intake @15:51:34.12 CBV.dial between AIV and waste1 @15:51:34.49 CSV.dial :air @15:51:34.97 CS.seek 10ml @15:52:01.46 CSV.dial CTV @15:52:01.94 CS.seek 0ml @15:52:28.46 CBV.dial :waste1 @15:52:28.81 delay 10 seconds @15:52:38.84 CTV.dial :air,avoiding: :intake @15:52:40.19 Storing lyfil puck in Collection Clamp to tube 1 @15:52:40.40 SC.reconfigure SCtight @15:52:40.47 SC.seek 1 @15:52:42.56 SC.reconfigure SCloose @15:52:42.82 Elbow.reconfigure TightElbow @15:52:42.89 Elbow.seek 1 @15:52:44.82 Forearm.reconfigure TightForearm @15:52:44.90 Forearm.seek 1 + 700 counts @15:52:45.98 SE.reconfigure SEpick @15:52:46.08 SE.seek beamBreak @15:52:48.80 SE.configure SEconfig @15:52:48.99 Elbow.reconfigure LimpElbow @15:52:49.06 Forearm.reconfigure LooseForearm @15:52:49.13 Forearm.seek 1 @15:52:49.49 Elbow.hold @15:52:49.59 Elbow.reconfigure TightElbow @15:52:49.73 SE.seek 3.08 Pucks below Top Plate @15:52:50.26 Forearm.force -20 @15:52:50.37 Forearm.seek 1 + 321 counts @15:52:50.80 Forearm.reconfigure TightForearm @15:52:50.87 Forearm.seek 1 + 700 counts @15:52:51.25 SE.seek 4.08 Pucks below Top Plate @15:52:51.94 Forearm.reconfigure LooseForearm @15:52:52.01 Forearm.seek :retract @15:52:52.97 Elbow.seek CC @15:52:54.74 Elbow.reconfigure LooseElbow @15:52:54.86 Forearm.seek CC @15:52:55.88 Hand.close @15:52:56.46 CC.seek :open @15:53:00.93 Forearm.seek :retract @15:53:01.97 Elbow.reconfigure TightElbow @15:53:02.04 Elbow.seek 1 @15:53:04.04 Elbow.reconfigure LimpElbow @15:53:04.12 Forearm.seek 1 @15:53:05.16 Elbow.hold @15:53:05.26 Elbow.reconfigure TightElbow @15:53:05.33 Hand.open @15:53:06.59 Forearm.force -20 @15:53:06.66 Forearm.seek 1 + 346 counts @15:53:07.00 Forearm.reconfigure TightForearm @15:53:07.07 Forearm.seek 1 + 700 counts @15:53:07.43 SE.seek :down @15:53:08.58
Forearm.reconfigure LooseForearm @15:53:08.65 Forearm.seek :retract @15:53:09.88 SE.coast @15:53:09.95
SC.coast @15:53:10.01 PSV.dial :air @15:53:11.06 PS.seek 0.499ml @15:53:17.04 delay 3 seconds @15:53:20.06 PSV.dial :mixloop @15:53:20.51 PRV.dial :air @15:53:20.99 delay 3 seconds @15:53:24.02 PRV.dial CBV @15:53:24.47 CBV.dial AIV @15:53:24.95 PRV.dial between CBV and air @15:53:25.29 PRV.dial :air @15:53:25.70 PS.reconfigure PSslow @15:53:25.77 PS.seek 0.599ml @15:53:28.41 PRV.dial :waste2 @15:53:29.14 PS.reconfigure PSconfig @15:53:29.21 PS.seek :empty @15:53:36.22 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:53:36.32 Hand.open @15:53:36.71 Elbow.seek FlushPuck::Garage @15:53:38.75 Elbow.reconfigure LooseElbow @15:53:38.89 Forearm.seek :garage @15:53:39.94 Hand.close @15:53:40.71 Forearm.seek :retract @15:53:41.74 Elbow.reconfigure TightElbow @15:53:41.82 Elbow.seek CC @15:53:44.75 Elbow.reconfigure LooseElbow @15:53:44.88 Forearm.reconfigure TightForearm @15:53:44.95 Forearm.seek CC @15:53:46.31 CC.seek :closed @15:53:50.59 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.72 @15:53:50.69 Hand.open @15:53:51.40 Forearm.reconfigure LooseForearm @15:53:51.47 Forearm.seek :retract @15:53:52.60 Clearing PML for modified lysate @15:53:52.61 CTV.dial :air,avoiding: :intake @15:53:52.63 PRV.dial :waste2 @15:53:52.66 PTV.dial :PRV,avoiding: :puck @15:53:53.43 delay 2 seconds @15:53:55.45 PTV.dial :PRVmixing,avoiding: :puck @15:53:56.71 delay 2 seconds @15:53:58.76 PSV.dial :PML @15:53:58.78 delay 2 seconds @15:54:00.81 PRV.dial :air @15:54:01.55 delay 2 seconds @15:54:03.64 PS.seek 1.499ml @15:54:19.69 delay 5 seconds @15:54:24.73 PS.seek 2.5ml @15:54:35.69 PSV.dial :air @15:54:36.17 PS.seek 0ml @15:55:02.38 mixing lysate with speDil @15:55:02.39 PSV.dial :PML @15:55:02.88 CBV.dial :PRV @15:55:02.89 PRV.dial :air @15:55:02.93 delay 2 seconds @15:55:04.96 PRV.dial :speDil @15:55:05.43 delay 2 seconds @15:55:07.51 PS.reconfigure PSslow1 @15:55:07.59 PS.seek 0.225ml @15:55:31.16 delay 5 seconds @15:55:36.19 PRV.dial CBV @15:55:36.94 delay 2 seconds @15:55:39.02 PS.seek 0.475ml @15:56:04.73 delay 5 seconds @15:56:09.76 PRV.dial :air @15:56:10.24 delay 2 seconds @15:56:12.27 PS.seek 0.965ml @15:57:02.32 delay 10 seconds @15:57:12.35 PRV.dial CBV @15:57:12.84 CTV.dial :air,avoiding: :intake @15:57:12.86 PSV.dial :air @15:57:13.34 PS.reconfigure PSconfig @15:57:13.42 PS.seek 2.5ml @15:57:29.91 delay 3 seconds @15:57:32.93 PSV.dial PTV @15:57:33.66 PTV.dial :PRV,avoiding: :puck @15:57:34.41 PS.seek 0ml @15:58:00.54 PSV.dial :air @15:58:01.27 PS.seek 2.5ml @15:58:27.48 delay 3 seconds @15:58:30.50 PSV.dial PTV @15:58:31.23 PTV.dial :PRV,avoiding: :puck @15:58:31.26 PS.seek 0ml @15:58:57.48 Pushing modified lysate into AIV Injection Loop @15:58:57.49 CBV.dial AIV @15:58:57.52 AIV.dial :bypass @15:58:57.56 PRV.dial CBV @15:58:57.59 PSV.dial :air @15:58:58.33 PS.seek 2.5ml @15:59:24.51 delay 3 seconds @15:59:27.53 PSV.dial :PML @15:59:27.98 PTV.dial :PRVmixing,avoiding: :puck @15:59:28.80 PS.reconfigure PSslow1 @15:59:28.87 PS.seek 1.74ml @16:00:46.23 delay 5 seconds @16:00:51.26 AIV.dial :sample @16:00:51.85 AWV.select :waste @16:00:52.05 PS.reconfigure PSslow2 @16:00:52.13 PS.seek 1.22ml @16:02:40.36 delay 15 seconds @16:02:55.39 AIV.dial :bypass @16:02:55.96 PS.reconfigure PSconfig @16:02:56.04 PS.seek 0ml @16:03:09.54 PSV.dial :air @16:03:10.01 PS.seek 2.5ml @16:03:36.11 PSV.dial :PML @16:03:36.57 PS.seek 0ml @16:04:02.70 AIV.dial :coilToMFB @16:04:02.71 AWV.select :air @16:04:02.92 Extracting Nucleic Acid from AIV @16:04:02.95
Beginning samplePassD @16:04:02.99 SPE.hold 2:46:40,55C @16:04:03.09 ASV.dial :SPEdelivery @16:04:03.24
AWV.select :air @16:04:03.26 AIV.dial :bypass @16:04:03.29 PSV.dial PSR @16:04:03.55 delay 20 seconds @16:04:04.91
Pulling 1.5ml of PSR.killMFB @16:04:04.92 PSR.select :killMFB @16:04:05.09 PS.seek 1.5ml @16:04:21.08 delay 5 seconds @16:04:23.58 primeLysateINT @16:04:23.59 AWV.select :air @16:04:23.63 AIV.dial :coilToMFB @16:04:23.66 ATV.dial AIV @16:04:25.33 AS.reconfigure ASslow1 @16:04:25.40 AS.seek 0.299ml @16:04:26.11
PSV.dial :waste1 @16:04:26.60 PS.seek 0ml @16:04:42.58 PSV.dial PSR @16:04:43.06 PSR.select :air @16:04:43.21 PS.seek 2.5ml @16:05:09.39 PRV.dial CBV @16:05:09.42
CBV.dial PRV @16:05:09.46 PSV.dial :mixloop @16:05:10.48 CTV.dial :air,avoiding: :intake @16:05:10.50 PTV.dial :PRVmixing @16:05:10.55 PS.seek 0ml @16:05:26.09 delay 10 seconds @16:05:36.12 ATV.dial :air2 @16:05:36.76
PSV.dial PSR @16:05:36.95 AS.seek 0.329ml @16:05:37.84
Pulling 1.5ml of PSR.flush @16:05:37.85 PSR.select :flush @16:05:38.02 PS.seek 1.5ml @16:05:43.29 ATV.dial :waste2 @16:05:43.85 AS.reconfigure ASnorm @16:05:43.92 AS.seek :empty @16:05:54.08
delay 5 seconds @16:05:59.11 PSV.dial :waste1 @16:05:59.73 PS.seek 0ml @16:06:00.93 Lysate primed! @16:06:00.94 AFV.select :holdingCoil @16:06:00.98 ATV.dial :air2 @16:06:01.61 AS.reconfigure ASslow1 @16:06:01.68 AS.seek 0.069ml @16:06:15.70
PSV.dial PSR @16:06:16.12 delay 3 seconds @16:06:16.17
PSR.select :air @16:06:16.32 PS.seek 2.5ml @16:06:19.15 ATV.dial :AIV @16:06:19.97 AS.seek 0.469ml @16:06:42.45 PRV.dial CBV @16:06:42.48
CBV.dial PRV @16:06:42.52 PSV.dial :mixloop @16:06:43.53 CTV.dial :air,avoiding: :intake @16:06:43.55 PTV.dial :PRVmixing @16:06:43.59 PS.seek 0ml @16:07:09.77 PSV.dial PSR @16:07:10.83 Pulling 1.5ml of PSR.flush @16:07:10.84 PSR.select :flush @16:07:11.01 PS.seek 1.5ml @16:07:27.02 delay 5 seconds @16:07:32.05 PSV.dial :waste1 @16:07:32.54 PS.seek 0ml @16:07:40.71 delay 5 seconds @16:07:45.80 Solid Phase Extraction Heater at 52.8C @16:07:45.81 ATV.dial ASV @16:07:46.23 ASV.dial :SPEdelivery @16:07:46.33 AS.seek 0.041ml @16:07:48.55
PSV.dial PSR @16:07:49.02 PSR.select :air @16:07:49.17 PS.seek 2.5ml @16:08:15.27 PRV.dial CBV @16:08:15.30
CBV.dial PRV @16:08:15.34 PSV.dial :mixloop @16:08:16.34 CTV.dial :air,avoiding: :intake @16:08:16.36 PTV.dial :PRVmixing @16:08:16.40 PS.seek 0ml @16:08:42.61 PSV.dial PSR @16:08:43.65 Pulling 1.5ml of PSR.flush @16:08:43.67 PSR.select :flush @16:08:43.84 PS.seek 1.5ml @16:08:59.83 delay 5 seconds @16:09:04.86 PSV.dial :waste1 @16:09:05.87 PS.seek 0ml @16:09:12.70 delay 10 seconds @16:09:22.05
PSV.dial PSR @16:09:22.52 PSR.select :air @16:09:22.67 PS.seek 2.5ml @16:09:22.74 rinseLysateLineINT @16:09:22.75 AFV.select :carrierSolution @16:09:22.88 delay 2 seconds @16:09:24.91 AS.reconfigure ASconfig @16:09:24.98 AS.seek 1ml @16:09:48.80 PRV.dial CBV @16:09:48.83
CBV.dial PRV @16:09:48.87 PSV.dial :mixloop @16:09:49.86 CTV.dial :air,avoiding: :intake @16:09:49.88 PTV.dial :PRVmixing @16:09:49.95 PS.reconfigure PSslow @16:09:50.07 PS.seek 1.2ml @16:10:16.89 PSV.dial :waste1 @16:10:18.14 PS.reconfigure PSconfig @16:10:18.22 PS.seek 0ml @16:10:20.45 delay 1 second @16:10:21.47 AFV.select :holdingCoil @16:10:21.59 delay 1 second @16:10:22.62 ATV.dial AIV @16:10:23.17 AIV.dial :coilToMFB @16:10:23.19 AWV.select :waste @16:10:23.34 AS.reconfigure ASslow0 @16:10:23.40 AS.seek 0ml @16:10:31.30
PSV.dial :air @16:10:32.29 PS.seek 2.5ml @16:10:58.49 PSV.dial :mixloop @16:10:58.94 PS.reconfigure PSslow @16:10:59.01 PS.seek 0ml @16:11:50.14 PSV.dial :air @16:11:50.61 PS.reconfigure PSconfig @16:11:50.69 PS.seek 2.5ml @16:12:04.29 ATV.dial :air2 @16:12:04.97 AS.reconfigure ASslow1 @16:12:05.04 AS.seek 0.05ml @16:12:15.43 ATV.dial :AIV @16:12:16.27 AS.seek 0.01ml @16:12:16.93
PSV.dial :mixloop @16:12:17.40 PS.reconfigure PSslow @16:12:17.47 PS.seek 0ml @16:12:24.64 ATV.dial :waste @16:12:26.32 AS.seek :empty @16:12:28.79 ethanol wash @16:12:28.80 ATV.dial :air2 @16:12:30.05 AS.reconfigure ASslow2 @16:12:30.13 AS.seek 0.009ml @16:12:40.65 delay 3 seconds @16:12:43.68 Pulling 0.4ml of Ethanol Wash @16:12:43.69 ATV.dial :ethanol @16:12:44.52 AS.reconfigure ASslow1 @16:12:44.59 AS.seek 0.409ml @16:13:08.55
Cleaning Collection Syringe @16:13:08.56 CSV.dial CSR @16:13:09.09 Pulling 4ml of CSR.flush @16:13:09.11 CSR.select :flush @16:13:09.26 CS.seek 4ml @16:13:20.51 delay 5 seconds @16:13:25.54 CSR.select :air @16:13:25.70 CS.seek 10ml @16:13:41.95 CSV.dial CTV @16:13:41.99
CTV.dial CSV @16:13:43.33 CBV.dial :waste1 @16:13:43.84 CS.seek 0ml @16:14:05.38 delay 3 seconds @16:14:08.40 ATV.dial :air1 @16:14:08.81 delay 3 seconds @16:14:10.36
CSV.dial CSR @16:14:10.91 Pulling 4ml of CSR.flush @16:14:10.92 CSR.select :flush @16:14:11.08 CS.seek 4ml @16:14:11.89 AS.seek 0.434ml @16:14:17.22 delay 3 seconds @16:14:20.24 ATV.dial :ethanol @16:14:20.86 AS.seek 0.419ml @16:14:22.32
delay 5 seconds @16:14:24.15 delay 3 seconds @16:14:27.18 ATV.dial ASV @16:14:27.79
CSR.select :air @16:14:27.93 CS.seek 10ml @16:14:29.02 AS.seek 0.092ml @16:14:44.27 CSV.dial CTV @16:14:44.31
CTV.dial CSV @16:14:44.80 CBV.dial :waste1 @16:14:44.83 CS.seek 0ml @16:15:11.59 Garaging FlushPuck in Collection Clamp @16:15:11.70 Elbow.reconfigure TightElbow @16:15:11.77 Elbow.seek CC @16:15:12.07 Elbow.reconfigure LooseElbow @16:15:12.19 Forearm.seek CC @16:15:13.22 Hand.close @16:15:13.87 CC.seek :open @16:15:18.24 Forearm.seek :retract @16:15:19.27 Elbow.reconfigure TightElbow @16:15:19.35 Elbow.seek :garage @16:15:22.46 Elbow.reconfigure LooseElbow @16:15:22.60 Forearm.reconfigure TightForearm @16:15:22.67 Forearm.seek :garage @16:15:23.83 Hand.open @16:15:24.56 Forearm.reconfigure LooseForearm @16:15:24.63 Forearm.seek :retract @16:15:25.66 Whole Cell Archive sampling 1500ml @16:15:25.68 Loading wcr puck from tube 2 into Collection Clamp @16:15:25.99 SC.reconfigure SCtight @16:15:26.07 SC.seek 2 @16:15:28.23 SC.reconfigure SCloose @16:15:28.48 Elbow.reconfigure TightElbow @16:15:28.56 Elbow.seek 2 @16:15:30.79 Forearm.reconfigure TightForearm @16:15:30.87 Forearm.seek 2 + 700 counts @16:15:31.95 SE.reconfigure SEpick @16:15:32.06 SE.seek beamBreak @16:15:33.55 SE.configure SEconfig @16:15:33.74 Elbow.seek 2 @16:15:33.98 Forearm.seek 2 + 300 counts @16:15:34.27 Forearm.reconfigure LooseForearm @16:15:34.34 Hand.close @16:15:35.01 SE.seek :down @16:15:35.23 delay 3 seconds @16:15:35.34 Forearm.seek :retract @16:15:36.33 Elbow.seek CC @16:15:36.42 SE.coast @16:15:37.93 Elbow.reconfigure LooseElbow @16:15:38.07 Forearm.reconfigure TightForearm @16:15:38.14 Forearm.seek CC @16:15:38.26 ATV.dial :waste2 @16:15:39.00 AS.reconfigure ASconfig @16:15:39.07 AS.seek :empty @16:15:40.18 CC.seek :closed @16:15:44.54 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.70 @16:15:44.56 Hand.open @16:15:44.74 ATV.dial :air2 @16:15:45.15 AS.reconfigure ASslow1 @16:15:45.24 AS.seek 1ml @16:15:45.40 Forearm.reconfigure LooseForearm @16:15:45.47 Forearm.seek :retract @16:15:46.52 SC.coast @16:15:46.59 collectSample 1500ml w/bubble point of 55psi @16:15:46.60 Priming 7ml @16:15:46.63 Intake.select :close @16:15:46.65 Exhaust.select :close @16:15:46.69 SS.hold @16:15:46.95 Calibrating pressure sensors @16:15:46.97 CTV.dial :air @16:15:48.25 SSV.dial :spit,avoiding: :exhaust @16:15:48.29 SS.reconfigure SSfast @16:15:48.37 SS.seek 5ml @16:15:50.97 SSV.dial CBV @16:15:51.01 CBV.dial SSV @16:15:52.38 delay 3.8 seconds @16:15:56.32 SSV.dial :spit @16:15:57.60 SS.seek 0ml @16:16:00.22 SSV.dial CBV @16:16:01.52 CTV.dial :intake @16:16:02.27 SSV.dial CBV @16:16:02.30 CBV.dial SSV @16:16:02.33 Allowing 40s for 7.0ml of remaining 7.0ml @16:16:02.37 Intake.select :open @16:16:04.57 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:16:04.60 SS.reconfigure SSinhale.with maxSpeed: 150 @16:16:04.69 SS.seek 7ml @16:16:07.73 Vacuum too high -- waiting up to 40s @16:16:10.63 Intake @34.6psia, vacuum from 20.0 to 24.0psi @16:16:10.65 SS.reconfigure SSinhale.with maxSpeed: 135 @16:16:10.75 SS.seek 7ml @16:16:13.41 Vacuum too high -- waiting up to 40s @16:16:16.58 SS.reconfigure SSinhale.with maxSpeed: 122 @16:16:16.67 SS.seek 7ml @16:16:18.92 Stroke completed -- waiting for pressure to equalize ... @16:16:24.05 Intake.select :close @16:16:24.24 SSV.dial :exhaust @16:16:25.03 Exhaust.select :open @16:16:25.17 SS.reconfigure SSexhale @16:16:25.25 SS.seek 0ml @16:16:29.08 Exhaust.select :close @16:16:29.22 Sampling 1500.0ml @16:16:29.24 Intake.select :close @16:16:29.26 Exhaust.select :close @16:16:29.29 SS.reconfigure SSconfig @16:16:29.37 SS.hold @16:16:29.62 CTV.dial :intake @16:16:29.64 SSV.dial CBV @16:16:29.68 CBV.dial SSV @16:16:30.45 Allowing 130s for 25.0ml of remaining 1500.0ml @16:16:30.47 Intake.select :open @16:16:32.69 Intake @32.1psia, vacuum from 20.0 to 24.0psi @16:16:32.72 SS.reconfigure SSinhale.with maxSpeed: 122 @16:16:32.81 SS.seek 25ml @16:16:36.35 Vacuum too high -- waiting up to 40s @16:16:39.69 Intake @35.2psia, vacuum from 20.0 to 24.0psi @16:16:39.71 SS.reconfigure SSinhale.with maxSpeed: 110 @16:16:39.81 SS.seek 25ml @16:16:42.04 Vacuum too high -- waiting up to 40s @16:16:43.64 Intake @34.4psia, vacuum from 20.0 to 24.0psi @16:16:43.67 SS.reconfigure SSinhale.with maxSpeed: 99 @16:16:43.76 SS.seek 25ml @16:16:46.13 Vacuum too high -- waiting up to 40s @16:16:47.75 Intake @34.5psia, vacuum from 20.0 to 24.0psi @16:16:47.78 SS.reconfigure SSinhale.with maxSpeed: 89 @16:16:47.87 SS.seek 25ml @16:16:50.00 Vacuum too high -- waiting up to 40s @16:16:51.88 Intake @34.3psia, vacuum from 20.0 to 24.0psi @16:16:51.93 SS.reconfigure SSinhale.with maxSpeed: 80 @16:16:52.09 SS.seek 25ml @16:16:54.76 Vacuum too high -- waiting up to 40s @16:16:56.39 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:16:56.41 SS.reconfigure SSinhale.with maxSpeed: 72 @16:16:56.50 SS.seek 25ml @16:16:58.94 Vacuum too high -- waiting up to 40s @16:17:00.55 Intake @34.2psia, vacuum from 20.0 to 24.0psi @16:17:00.58 SS.reconfigure SSinhale.with maxSpeed: 65 @16:17:00.67 SS.seek 25ml @16:17:03.41 Vacuum too high -- waiting up to 40s @16:17:04.76 Intake @34.1psia, vacuum from 20.0 to 24.0psi @16:17:04.79 SS.reconfigure SSinhale.with maxSpeed: 59 @16:17:04.88 SS.seek 25ml @16:17:07.51 Vacuum too high -- waiting up to 40s @16:17:08.85 SS.reconfigure SSinhale.with maxSpeed: 53 @16:17:08.93 SS.seek 25ml @16:17:11.66 Vacuum too high -- waiting up to 40s @16:17:12.99 Intake @34.0psia, vacuum from 20.0 to 24.0psi @16:17:13.01 SS.reconfigure SSinhale.with maxSpeed: 48 @16:17:13.10 SS.seek 25ml @16:17:16.38 Vacuum too high -- waiting up to 40s @16:17:17.71 Intake @33.9psia, vacuum from 20.0 to 24.0psi @16:17:17.74 SS.reconfigure SSinhale.with maxSpeed: 43 @16:17:17.83 SS.seek 25ml @16:17:22.35 Vacuum too high -- waiting up to 40s @16:17:23.68 SS.reconfigure SSinhale.with maxSpeed: 39 @16:17:23.76 SS.seek 25ml @16:17:28.49 Stroke completed -- waiting for pressure to equalize ... @16:17:32.90 Intake.select :close @16:17:33.09 SSV.dial :exhaust @16:17:33.89 Exhaust.select :open @16:17:34.04 SS.reconfigure SSexhale @16:17:34.11 SS.seek 0ml @16:17:43.41 Exhaust.select :close @16:17:43.56 SSV.dial CBV @16:17:43.59 CBV.dial SSV @16:17:44.36 Allowing 130s for 25.0ml of remaining 1475.0ml @16:17:44.38 Intake.select :open @16:17:46.60 Intake @32.0psia, vacuum from 20.0 to 24.0psi @16:17:46.62 SS.reconfigure SSinhale.with maxSpeed: 39 @16:17:46.72 SS.seek 25ml @16:18:03.73 Vacuum too high -- waiting up to 40s @16:18:05.37 Intake @33.6psia, vacuum from 20.0 to 24.0psi @16:18:05.41 SS.reconfigure SSinhale.with maxSpeed: 35 @16:18:05.52 SS.seek 25ml @16:18:20.48 Vacuum too high -- waiting up to 40s @16:18:21.58 Intake @33.4psia, vacuum from 20.0 to 24.0psi @16:18:21.61 SS.reconfigure SSinhale.with maxSpeed: 32 @16:18:21.70 SS.seek 25ml @16:18:40.72 Stroke completed -- waiting for pressure to equalize ... @16:18:45.18 Intake.select :close @16:18:45.37 SSV.dial :exhaust @16:18:46.17 Exhaust.select :open @16:18:46.31 SS.reconfigure SSexhale @16:18:46.38 SS.seek 0ml @16:18:55.05 Exhaust.select :close @16:18:55.20 SSV.dial CBV @16:18:55.23 CBV.dial SSV @16:18:55.99 Allowing 130s for 25.0ml of remaining 1450.0ml @16:18:56.02 Intake.select :open @16:18:58.22 Intake @31.9psia, vacuum from 20.0 to 24.0psi @16:18:58.24 SS.reconfigure SSinhale.with maxSpeed: 32 @16:18:58.33 SS.seek 25ml @16:19:06.86 delay 5 seconds @16:19:11.89 ATV.dial ASV @16:19:12.86 ASV.dial :SPEdelivery @16:19:12.89 AS.seek 0.02ml @16:19:53.43 Stroke completed -- waiting for pressure to equalize ... @16:19:57.90 Intake.select :close @16:19:58.10 SSV.dial :exhaust @16:19:59.45 Exhaust.select :open @16:19:59.60 SS.reconfigure SSexhale @16:19:59.67 SS.seek 0ml @16:20:08.33 Exhaust.select :close @16:20:08.48 SSV.dial CBV @16:20:08.51 CBV.dial SSV @16:20:09.30 Allowing 130s for 25.0ml of remaining 1425.0ml @16:20:09.33 Intake.select :open @16:20:11.52 Intake @31.9psia, vacuum from 20.0 to 24.0psi @16:20:11.55 SS.reconfigure SSinhale.with maxSpeed: 32 @16:20:11.64 SS.seek 25ml @16:21:06.72 Stroke completed -- waiting for pressure to equalize ... @16:21:11.86 Intake.select :close @16:21:12.04 SSV.dial :exhaust @16:21:12.83 Exhaust.select :open @16:21:12.98 SS.reconfigure SSexhale @16:21:13.05 SS.seek 0ml @16:21:22.00 Exhaust.select :close @16:21:22.15 SSV.dial CBV @16:21:22.18 CBV.dial SSV @16:21:22.99 Allowing 130s for 25.0ml of remaining 1400.0ml @16:21:23.01 Intake.select :open @16:21:25.24 Intake @31.8psia, vacuum from 20.0 to 24.0psi @16:21:25.26 SS.reconfigure SSinhale.with maxSpeed: 32 @16:21:25.35 SS.seek 25ml @16:22:20.11 Stroke completed -- waiting for pressure to equalize ... @16:22:26.46 Intake.select :close @16:22:26.64 SSV.dial :exhaust @16:22:27.41 Exhaust.select :open @16:22:27.56 SS.reconfigure SSexhale @16:22:27.71 SS.seek 0ml @16:22:30.29 delay 5 seconds @16:22:35.32 ATV.dial :air2 @16:22:36.35 AS.seek 0.52ml @16:22:36.75 Exhaust.select :close @16:22:36.89 SSV.dial CBV @16:22:36.92 CBV.dial SSV @16:22:37.70 Allowing 130s for 25.0ml of remaining 1375.0ml @16:22:37.72 Intake.select :open @16:22:39.94 Intake @31.8psia, vacuum from 20.0 to 24.0psi @16:22:39.96 SS.reconfigure SSinhale.with maxSpeed: 32 @16:22:40.05 SS.seek 25ml @16:23:34.55 Stroke completed -- waiting for pressure to equalize ... @16:23:41.43 Intake.select :close @16:23:41.60 SSV.dial :exhaust @16:23:42.38 Exhaust.select :open @16:23:42.52 SS.reconfigure SSexhale @16:23:42.59 SS.seek 0ml @16:23:51.24 Exhaust.select :close @16:23:51.39 SSV.dial CBV @16:23:51.42 CBV.dial SSV @16:23:52.19 Allowing 130s for 25.0ml of remaining 1350.0ml @16:23:52.21 Intake.select :open @16:23:54.43 Intake @31.6psia, vacuum from 20.0 to 24.0psi @16:23:54.45 SS.reconfigure SSinhale.with maxSpeed: 32 @16:23:54.54 SS.seek 25ml @16:24:17.21 delay 5 seconds @16:24:22.24 ATV.dial ASV @16:24:23.21 ASV.dial :wasteR @16:24:23.61 AS.seek 0.3ml @16:24:30.38 Vacuum too high -- waiting up to 40s @16:24:32.75 Intake @32.4psia, vacuum from 20.0 to 24.0psi @16:24:32.77 SS.reconfigure SSinhale.with maxSpeed: 29 @16:24:32.87 SS.seek 25ml @16:24:54.44 Stroke completed -- waiting for pressure to equalize ... @16:25:00.77 Intake.select :close @16:25:00.95 SSV.dial :exhaust @16:25:01.75 Exhaust.select :open @16:25:01.89 SS.reconfigure SSexhale @16:25:01.96 SS.seek 0ml @16:25:08.16 delay 20 seconds @16:25:10.97 Exhaust.select :close @16:25:11.11 SSV.dial CBV @16:25:11.15 CBV.dial SSV @16:25:11.92 Allowing 130s for 25.0ml of remaining 1325.0ml @16:25:11.95 Intake.select :open @16:25:14.14 Intake @31.8psia, vacuum from 20.0 to 24.0psi @16:25:14.17 SS.reconfigure SSinhale.with maxSpeed: 29 @16:25:14.25 SS.seek 25ml @16:25:28.19 AS.seek 0.07ml @16:26:14.72 delay 20 seconds @16:26:14.77 Stroke completed -- waiting for pressure to equalize ... @16:26:22.38 Intake.select :close @16:26:23.12 SSV.dial :exhaust @16:26:23.92 Exhaust.select :open @16:26:24.07 SS.reconfigure SSexhale @16:26:24.14 SS.seek 0ml @16:26:32.83 Exhaust.select :close @16:26:32.98 SSV.dial CBV @16:26:33.01 CBV.dial SSV @16:26:33.80 Allowing 130s for 25.0ml of remaining 1300.0ml @16:26:33.83 Intake.select :open @16:26:34.74 ATV.dial :waste2 @16:26:35.47 AS.reconfigure ASconfig @16:26:35.54 AS.seek :empty @16:26:36.04 Intake @31.8psia, vacuum from 20.0 to 24.0psi @16:26:36.07 SS.reconfigure SSinhale.with maxSpeed: 29 @16:26:36.15 SS.seek 25ml @16:26:39.93 Elution @16:26:39.94 ATV.dial :air1 @16:26:41.06 AS.reconfigure ASslow1 @16:26:41.13 AS.seek 0.049ml @16:26:51.53 delay 3 seconds @16:26:54.56 ATV.dial :ElutionWater @16:26:55.25 AS.seek 0.109ml @16:27:07.60 delay 3 seconds @16:27:10.63 Vacuum too high -- waiting up to 40s @16:27:10.67 ATV.dial ASV,via: :air2 @16:27:12.73 ASV.dial :SPEelution @16:27:13.04 Intake @32.5psia, vacuum from 20.0 to 24.0psi @16:27:13.06 SS.reconfigure SSinhale.with maxSpeed: 26 @16:27:13.15 SS.seek 25ml @16:27:13.32 AFV.select :carrierSolution @16:27:13.48 AS.reconfigure ASnorm @16:27:13.55 AS.seek 0.759ml @16:27:43.10 Stroke completed -- waiting for pressure to equalize ... @16:27:46.64 delay 3 seconds @16:27:49.66 AFV.select :holdingCoil @16:27:49.84 AS.reconfigure ASslow1 @16:27:49.91 AS.seek 0.474ml @16:27:50.19 Intake.select :close @16:27:50.37 SSV.dial :exhaust @16:27:51.17 Exhaust.select :open @16:27:51.31 SS.reconfigure SSexhale @16:27:51.38 SS.seek 0ml @16:28:00.36 Exhaust.select :close @16:28:00.51 SSV.dial CBV @16:28:00.54 CBV.dial SSV @16:28:01.34 Allowing 130s for 25.0ml of remaining 1275.0ml @16:28:01.36 Intake.select :open @16:28:03.56 Intake @31.7psia, vacuum from 20.0 to 24.0psi @16:28:03.59 SS.reconfigure SSinhale.with maxSpeed: 26 @16:28:03.69 SS.seek 25ml @16:28:47.66 delay 5 seconds @16:28:52.76 AS.reconfigure ASslow2 @16:28:52.83 AS.seek 0.439ml @16:29:11.14 Stroke completed -- waiting for pressure to equalize ... @16:29:19.98 Intake.select :close @16:29:20.17 SSV.dial :exhaust @16:29:20.97 Exhaust.select :open @16:29:21.13 SS.reconfigure SSexhale @16:29:21.20 SS.seek 0ml @16:29:28.30 delay 1:00 @16:29:29.92 Exhaust.select :close @16:29:30.06 SSV.dial CBV @16:29:30.09 CBV.dial SSV @16:29:30.88 Allowing 130s for 25.0ml of remaining 1250.0ml @16:29:30.90 Intake.select :open @16:29:33.12 Intake @31.9psia, vacuum from 20.0 to 24.0psi @16:29:33.14 SS.reconfigure SSinhale.with maxSpeed: 26 @16:29:33.24 SS.seek 25ml @16:30:22.82 Vacuum too high -- waiting up to 40s @16:30:25.93 Intake @32.4psia, vacuum from 20.0 to 24.0psi @16:30:25.96 SS.reconfigure SSinhale.with maxSpeed: 23 @16:30:26.05 SS.seek 25ml @16:30:28.33 pushing eluate to holding coil @16:30:28.44 AS.seek 0.279ml @16:30:46.66 Stroke completed -- waiting for pressure to equalize ... @16:30:54.88 Intake.select :close @16:30:55.07 SSV.dial :exhaust @16:30:55.86 Exhaust.select :open @16:30:56.00 SS.reconfigure SSexhale @16:30:56.07 SS.seek 0ml @16:31:04.77 Exhaust.select :close @16:31:04.92 SSV.dial CBV @16:31:04.95 CBV.dial SSV @16:31:05.74 Allowing 130s for 25.0ml of remaining 1225.0ml @16:31:05.76 Intake.select :open @16:31:08.53 Intake @31.8psia, vacuum from 20.0 to 24.0psi @16:31:08.56 SS.reconfigure SSinhale.with maxSpeed: 23 @16:31:08.65 SS.seek 25ml @16:32:24.92 Stroke completed -- waiting for pressure to equalize ... @16:32:35.10 Intake.select :close @16:32:35.28 SSV.dial :exhaust @16:32:36.08 Exhaust.select :open @16:32:36.23 SS.reconfigure SSexhale @16:32:36.30 SS.seek 0ml @16:32:45.27 Exhaust.select :close @16:32:45.42 SSV.dial CBV @16:32:45.45 CBV.dial SSV @16:32:46.24 Allowing 130s for 25.0ml of remaining 1200.0ml @16:32:46.26 Intake.select :open @16:32:48.48 Intake @31.9psia, vacuum from 20.0 to 24.0psi @16:32:48.50 SS.reconfigure SSinhale.with maxSpeed: 23 @16:32:48.59 SS.seek 25ml @16:33:09.60 delay 30 seconds @16:33:38.34 Vacuum too high -- waiting up to 40s @16:33:39.63 ATV.dial :waste,via: :air3 @16:33:41.26 AS.reconfigure ASconfig @16:33:41.33 AS.seek :empty @16:33:42.08 Intake @32.3psia, vacuum from 20.0 to 24.0psi @16:33:42.11 SS.reconfigure SSinhale.with maxSpeed: 21 @16:33:42.20 SS.seek 25ml @16:33:57.71 Parking Bleach in LysateSource line and SPEcolumn @16:33:57.72 ATV.dial :air3 @16:33:58.58 AS.reconfigure ASslow1 @16:33:58.65 AS.seek 0.029ml @16:34:05.04 ATV.dial :ElutionWater @16:34:05.66 AS.seek 0.059ml @16:34:11.39 Stroke completed -- waiting for pressure to equalize ... @16:34:11.99 ATV.dial :air3 @16:34:12.66 AS.seek 0.069ml @16:34:15.22 delay 2 seconds @16:34:17.32 ATV.dial :bleach @16:34:17.78 AS.seek 0.769ml @16:34:21.84 Intake.select :close @16:34:22.03 SSV.dial :exhaust @16:34:22.80 Exhaust.select :open @16:34:22.94 SS.reconfigure SSexhale @16:34:23.01 SS.seek 0ml @16:34:32.08 Exhaust.select :close @16:34:32.23 SSV.dial CBV @16:34:32.26 CBV.dial SSV @16:34:33.05 Allowing 130s for 25.0ml of remaining 1175.0ml @16:34:33.07 Intake.select :open @16:34:35.29 Intake @31.9psia, vacuum from 20.0 to 24.0psi @16:34:35.31 SS.reconfigure SSinhale.with maxSpeed: 21 @16:34:35.40 SS.seek 25ml @16:35:33.13 Vacuum too high -- waiting up to 40s @16:35:36.99 Intake @32.3psia, vacuum from 20.0 to 24.0psi @16:35:37.02 SS.reconfigure SSinhale.with maxSpeed: 19 @16:35:37.11 SS.seek 25ml @16:36:06.13 Stroke completed -- waiting for pressure to equalize ... @16:36:16.88 Intake.select :close @16:36:17.63 SSV.dial :exhaust @16:36:18.39 Exhaust.select :open @16:36:18.53 SS.reconfigure SSexhale @16:36:18.60 SS.seek 0ml @16:36:27.44 Exhaust.select :close @16:36:27.59 SSV.dial CBV @16:36:27.62 CBV.dial SSV @16:36:28.39 Allowing 130s for 25.0ml of remaining 1150.0ml @16:36:28.42 Intake.select :open @16:36:30.62 Intake @31.9psia, vacuum from 20.0 to 24.0psi @16:36:30.64 SS.reconfigure SSinhale.with maxSpeed: 19 @16:36:30.73 SS.seek 25ml @16:36:38.85 delay 3 seconds @16:36:41.88 ATV.dial :air3 @16:36:42.43 delay 3 seconds @16:36:45.52 AS.seek 0.794ml @16:36:50.85 delay 3 seconds @16:36:53.88 ATV.dial :bleach @16:36:54.50 AS.seek 0.779ml @16:36:57.84 delay 3 seconds @16:37:00.87 ATV.dial :AIV @16:37:02.09 AS.reconfigure ASconfig @16:37:02.17 AS.seek 0.015ml @16:37:43.13 Vacuum too high -- waiting up to 40s @16:37:46.43 ATV.dial :waste @16:37:47.99 AS.seek :empty @16:37:48.15 Intake @32.2psia, vacuum from 20.0 to 24.0psi @16:37:48.18 SS.reconfigure SSinhale.with maxSpeed: 17 @16:37:48.26 SS.seek 25ml @16:37:49.29 ATV.dial :air3 @16:37:49.97 AS.reconfigure ASslow1 @16:37:50.04 AS.seek 0.009ml @16:37:52.40 delay 2 seconds @16:37:54.42 ATV.dial :bleach @16:37:54.89 AS.seek 0.579ml @16:38:10.95 Stroke completed -- waiting for pressure to equalize ... @16:38:22.92 Intake.select :close @16:38:23.11 SSV.dial :exhaust @16:38:23.91 Exhaust.select :open @16:38:24.06 SS.reconfigure SSexhale @16:38:24.13 SS.seek 0ml @16:38:33.27 Exhaust.select :close @16:38:33.42 SSV.dial CBV @16:38:33.45 CBV.dial SSV @16:38:34.24 Allowing 130s for 25.0ml of remaining 1125.0ml @16:38:34.27 Intake.select :open @16:38:36.48 Intake @32.0psia, vacuum from 20.0 to 24.0psi @16:38:36.50 SS.reconfigure SSinhale.with maxSpeed: 17 @16:38:36.59 SS.seek 25ml @16:39:49.81 delay 3 seconds @16:39:52.84 ATV.dial :air3 @16:39:53.40 delay 3 seconds @16:39:56.50 AS.seek 0.604ml @16:40:01.83 delay 3 seconds @16:40:04.86 ATV.dial :bleach @16:40:05.49 AS.seek 0.589ml @16:40:08.81 delay 3 seconds @16:40:08.95 Vacuum too high -- waiting up to 40s @16:40:11.83 ATV.dial :ASV @16:40:12.82 ASV.dial :wasteR @16:40:13.41 AS.seek 0ml @16:40:14.19 Intake @32.2psia, vacuum from 20.0 to 24.0psi @16:40:14.21 SS.reconfigure SSinhale.with maxSpeed: 15 @16:40:14.30 SS.seek 25ml @16:40:26.58 Stroke completed -- waiting for pressure to equalize ... @16:40:38.51 Intake.select :close @16:40:38.68 SSV.dial :exhaust @16:40:39.44 Exhaust.select :open @16:40:39.59 SS.reconfigure SSexhale @16:40:39.66 SS.seek 0ml @16:40:48.58 Exhaust.select :close @16:40:48.73 SSV.dial CBV @16:40:48.76 CBV.dial SSV @16:40:49.55 Allowing 130s for 25.0ml of remaining 1100.0ml @16:40:49.58 Intake.select :open @16:40:51.78 Intake @32.0psia, vacuum from 20.0 to 24.0psi @16:40:51.81 SS.reconfigure SSinhale.with maxSpeed: 15 @16:40:51.90 SS.seek 25ml @16:42:12.14 delay 1:00 @16:42:49.18 Stroke completed -- waiting for pressure to equalize ... @16:42:59.58 SS.reconfigure SSconfig @16:42:59.66 SS.hold @16:42:59.81 Intake.select :close @16:42:59.99 ShallowSampler::Clogged in WCR -- progress too slow after 425.0ml @16:43:00.01 SSV.dial :exhaust @16:43:00.79 Exhaust.select :open @16:43:00.94 SS.reconfigure SSexhale @16:43:01.01 SS.seek 0ml @16:43:09.88 Exhaust.select :close @16:43:10.02 Sampled 425.0ml @16:43:10.05 Drying puck before fixing for 4:00 @16:43:10.07 CBV.dial :waste2b @16:43:10.84 delay 1:00 @16:43:12.17 AFV.select :carrierSolution @16:43:12.29 delay 2 seconds @16:43:14.32 AS.reconfigure ASconfig @16:43:14.39 AS.seek 1ml @16:44:10.87 CSV.dial :air @16:44:11.34 CS.reconfigure CSfast @16:44:11.42 CS.seek 10ml @16:44:12.22 delay 1 second @16:44:13.25 AFV.select :holdingCoil @16:44:13.38 delay 1 second @16:44:14.41 ATV.dial ASV @16:44:14.42 ASV.dial :wasteR @16:44:14.46 AS.reconfigure ASslow1 @16:44:14.54 AS.seek 0ml @16:44:33.17 Pumping wcr puck from tube 2 up to 55psi... @16:44:33.19 CBV.dial SSV @16:44:34.54 CBV.dial between CSV and waste2b @16:44:35.54 CSV.dial between air and CTV @16:44:35.90 CS.seek 7.158ml @16:44:42.99 CTV.dial CSV,avoiding: :air @16:44:43.02 CSV.dial CTV @16:44:43.85 CS.reconfigure CSpressurize @16:44:44.48 CS.seek 0ml @16:45:03.45 CSV.dial :air @16:45:03.93 CS.reconfigure CSfast @16:45:04.00 CS.seek 10ml @16:45:25.77 CSV.dial between air and CTV @16:45:26.13 CS.seek 2.528ml @16:45:42.93 CTV.dial CSV,avoiding: :air @16:45:42.96 CSV.dial CTV @16:45:43.32 CS.reconfigure CSpressurize @16:45:43.39 CS.seek 0ml @16:45:47.98 CBV.dial :waste2b @16:45:48.36 CS.reconfigure CSconfig @16:45:48.44 CS.seek :empty @16:45:51.77 delay 1:00 @16:46:51.80 CTV.dial :air,avoiding: :intake @16:46:53.10 Fix #1 of 2 (with rnal) for 2:00 @16:46:53.11 CSV.dial CSR @16:46:53.66 Pulling 2ml of CSR.rnal @16:46:53.68 CSR.select :rnal @16:46:53.84 CS.seek 2ml @16:47:00.05 delay 5 seconds @16:47:05.08 CSR.select :air @16:47:05.22 CS.reconfigure CSfast @16:47:05.29 CS.seek 10ml @16:47:22.80 Pumping wcr puck from tube 2 up to 55psi... @16:47:22.82 CBV.dial SSV @16:47:24.16 CBV.dial between CSV and waste2b @16:47:25.13 CSV.dial between air and CTV @16:47:25.76 CS.seek 7.158ml @16:47:32.87 CTV.dial CSV,avoiding: :air @16:47:32.90 CSV.dial CTV @16:47:34.24 CS.reconfigure CSpressurize @16:47:34.33 CS.seek 0ml @16:47:35.94 delay 20 seconds @16:47:53.27 CSV.dial :air @16:47:53.78 CS.reconfigure CSfast @16:47:53.85 CS.seek 10ml @16:47:55.97 ATV.dial :air3 @16:47:57.40 AS.seek 0.59ml @16:48:15.68 CSV.dial between air and CTV @16:48:16.04 CS.seek 2.177ml @16:48:33.50 CTV.dial CSV,avoiding: :air @16:48:33.53 CSV.dial CTV @16:48:33.89 CS.reconfigure CSpressurize @16:48:33.96 CS.seek 0ml @16:48:36.16 delay 2:00 @16:49:56.38 delay 3 seconds @16:49:59.41 ATV.dial :ASV @16:50:00.77 ASV.dial :wasteR @16:50:00.87 AS.seek 0.09ml @16:50:36.19 CBV.dial :waste2b @16:50:36.55 CS.reconfigure CSconfig @16:50:36.63 CS.seek :empty @16:50:41.58 delay 1:00 @16:51:41.61 CTV.dial :air,avoiding: :intake @16:51:41.71 delay 30 seconds @16:51:42.90 Fix #2 of 2 (with rnal) for 2:00 @16:51:43.45 CSV.dial CSR @16:51:44.00 Pulling 2ml of CSR.rnal @16:51:44.01 CSR.select :rnal @16:51:44.17 CS.seek 2ml @16:51:50.33 delay 5 seconds @16:51:55.36 CSR.select :air @16:51:55.51 CS.reconfigure CSfast @16:51:55.58 CS.seek 10ml @16:52:11.73 ATV.dial :extSrc @16:52:12.49 delay 3 seconds @16:52:13.12 Pumping wcr puck from tube 2 up to 55psi... @16:52:13.14 CBV.dial SSV @16:52:14.50 CBV.dial between CSV and waste2b @16:52:15.50 CSV.dial between air and CTV @16:52:15.59 ATV.dial :ElutionWater @16:52:16.13 CS.seek 7.145ml @16:52:16.67 AS.seek 0.12ml @16:52:23.02 ATV.dial :air3 @16:52:23.16 CTV.dial CSV,avoiding: :air @16:52:23.19 CSV.dial CTV @16:52:23.66 AS.seek 0.13ml @16:52:24.53 CS.reconfigure CSpressurize @16:52:24.61 CS.seek 0ml @16:52:25.95 delay 2 seconds @16:52:27.97 ATV.dial :bleach @16:52:28.46 AS.seek 0.23ml @16:52:43.55 CSV.dial :air @16:52:44.05 CS.reconfigure CSfast @16:52:44.12 CS.seek 10ml @16:52:48.92 delay 3 seconds @16:52:51.95 ATV.dial :air3 @16:52:52.50 delay 3 seconds @16:52:55.59 AS.seek 0.255ml @16:53:00.95 delay 3 seconds @16:53:03.98 ATV.dial :bleach @16:53:04.60 AS.seek 0.24ml @16:53:05.87 CSV.dial between air and CTV @16:53:06.24 CS.seek 2.194ml @16:53:07.92 delay 3 seconds @16:53:10.95 ATV.dial :ASV @16:53:11.97 ASV.dial :SPEdelivery @16:53:12.38 AFV.select :carrierSolution @16:53:12.51 delay 2 seconds @16:53:14.54 AS.reconfigure ASconfig @16:53:14.61 AS.seek 0.5ml @16:53:23.66 CTV.dial CSV,avoiding: :air @16:53:23.69 CSV.dial CTV @16:53:24.05 CS.reconfigure CSpressurize @16:53:24.13 CS.seek 0ml @16:53:26.42 delay 2:00 @16:53:29.88 delay 1 second @16:53:30.90 AFV.select :holdingCoil @16:53:31.07 AS.reconfigure ASslow1 @16:53:31.14 AS.seek 0.17ml @16:54:37.89 SPE.hold 2:46:40,95C @16:54:37.95 delay 3:00 @16:55:26.46 CBV.dial :waste2b @16:55:26.84 CS.reconfigure CSconfig @16:55:26.92 CS.seek :empty @16:55:31.94 delay 1:00 @16:56:31.97 CTV.dial :air,avoiding: :intake @16:56:33.38 Storing wcr puck in Collection Clamp to tube 1 @16:56:34.13 SC.reconfigure SCtight @16:56:34.22 SC.seek 1 @16:56:36.33 SC.reconfigure SCloose @16:56:36.59 Elbow.reconfigure TightElbow @16:56:36.67 Elbow.seek 1 @16:56:38.63 Forearm.reconfigure TightForearm @16:56:38.71 Forearm.seek 1 + 700 counts @16:56:39.75 SE.reconfigure SEpick @16:56:39.87 SE.seek beamBreak @16:56:42.50 SE.configure SEconfig @16:56:42.70 Elbow.reconfigure LimpElbow @16:56:42.78 Forearm.reconfigure LooseForearm @16:56:42.85 Forearm.seek 1 @16:56:43.16 Elbow.hold @16:56:43.26 Elbow.reconfigure TightElbow @16:56:43.39 SE.seek 4.07 Pucks below Top Plate @16:56:43.91 Forearm.force -20 @16:56:43.97 Forearm.seek 1 + 339 counts @16:56:44.33 Forearm.reconfigure TightForearm @16:56:44.41 Forearm.seek 1 + 700 counts @16:56:44.67 SE.seek 5.07 Pucks below Top Plate @16:56:45.36 Forearm.reconfigure LooseForearm @16:56:45.44 Forearm.seek :retract @16:56:46.43 Elbow.seek CC @16:56:48.17 Elbow.reconfigure LooseElbow @16:56:48.30 Forearm.seek CC @16:56:49.34 Hand.close @16:56:49.93 CC.seek :open @16:56:54.47 Forearm.seek :retract @16:56:55.55 Elbow.reconfigure TightElbow @16:56:55.63 Elbow.seek 1 @16:56:57.66 Elbow.reconfigure LimpElbow @16:56:57.74 Forearm.seek 1 @16:56:58.77 Elbow.hold @16:56:58.87 Elbow.reconfigure TightElbow @16:56:58.94 Hand.open @16:56:59.68 Forearm.force -20 @16:56:59.75 Forearm.seek 1 + 348 counts @16:57:00.10 Forearm.reconfigure TightForearm @16:57:00.18 Forearm.seek 1 + 700 counts @16:57:00.57 SE.seek :down @16:57:02.19 Forearm.reconfigure LooseForearm @16:57:02.35 Forearm.seek :retract @16:57:02.83 SE.coast @16:57:03.32 SC.coast @16:57:03.39 Starting finalPurge @16:57:03.41 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:57:03.52 Hand.open @16:57:03.94 Elbow.seek FlushPuck::Garage @16:57:06.00 Elbow.reconfigure LooseElbow @16:57:06.14 Forearm.seek :garage @16:57:07.18 Hand.close @16:57:07.94 Forearm.seek :retract @16:57:08.99 Elbow.reconfigure TightElbow @16:57:09.06 Elbow.seek CC @16:57:11.97 Elbow.reconfigure LooseElbow @16:57:12.11 Forearm.reconfigure TightForearm @16:57:12.18 Forearm.seek CC @16:57:13.52 CC.seek :closed @16:57:17.83 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.81 @16:57:17.94 Hand.open @16:57:18.62 Forearm.reconfigure LooseForearm @16:57:18.69 Forearm.seek :retract @16:57:19.76 Flushing the Collection Valve::Manifold @16:57:19.92 CSV.dial CSR @16:57:20.45 Pulling 5ml of CSR.flush @16:57:20.47 CSR.select :flush @16:57:20.62 CS.seek 5ml @16:57:34.40 delay 5 seconds @16:57:37.98 AFV.select :carrierSolution @16:57:38.11 delay 2 seconds @16:57:39.43
CSR.select :air @16:57:39.62 CS.reconfigure CSfast @16:57:39.69 CS.seek 7.5ml @16:57:40.13 AS.reconfigure ASconfig @16:57:40.20 AS.seek 1ml @16:57:46.12
CSV.dial :waste @16:57:47.40 CS.reconfigure CSconfig @16:57:47.48 CS.seek 0ml @16:58:07.65 Flushing lower manifold's waste1 port @16:58:07.81 CSV.dial :flush @16:58:08.28 CS.reconfigure CSfast @16:58:08.36 CS.seek 5ml @16:58:19.97 delay 5 seconds @16:58:25.00 CSV.dial :air @16:58:25.66 CS.seek 7ml @16:58:28.21 delay 1 second @16:58:29.23 AFV.select :holdingCoil @16:58:29.35 delay 1 second @16:58:30.38 ASV.dial :wasteD @16:58:31.09 CSV.dial CTV @16:58:31.13
CTV.dial CSV @16:58:31.37 AS.seek 0ml @16:58:32.51
CBV.dial :waste1 @16:58:33.30 CS.seek 0ml @16:58:49.43 Flushing lower manifold's waste2b port @16:58:49.59 CSV.dial :flush @16:58:50.91 CS.seek 5ml @16:59:02.50 delay 5 seconds @16:59:07.53 CSV.dial :air @16:59:08.11 CS.seek 7ml @16:59:13.47 CSV.dial CTV @16:59:13.51
CTV.dial CSV @16:59:13.98 CBV.dial :waste2b @16:59:14.79 CS.seek 0ml @16:59:29.16 Priming Eluate @16:59:29.17 AFV.select :carrierSolution @16:59:29.32 delay 2 seconds @16:59:30.56
Flushing upper manifold @16:59:30.72 CSV.dial :flush @16:59:31.36 AS.seek 0.5ml @16:59:31.48
CS.seek 5ml @16:59:43.03 delay 5 seconds @16:59:48.06 CSV.dial :air @16:59:48.60 CS.seek 7ml @16:59:53.87 CSV.dial CBV @16:59:53.91
CBV.dial CSV @16:59:55.00 CTV.dial :air @16:59:56.30 CS.seek 0ml @17:00:00.40 delay 1 second @17:00:01.42 AFV.select :holdingCoil @17:00:01.55 ATV.dial ASV @17:00:01.57 ASV.dial :SPErecovery @17:00:02.16 Swish #1 of 2 @17:00:02.26 AS.reconfigure ASslow0 @17:00:02.33 AS.seek 0.6ml @17:00:12.00
delay 7 seconds @17:00:12.81 AS.seek 0.5ml @17:00:19.04
CS.seek 3ml @17:00:23.26 Swish #2 of 2 @17:00:23.37 AS.seek 0.6ml @17:00:26.50
CSV.dial :waste @17:00:27.10 CS.seek 0ml @17:00:33.86 AS.seek 0.5ml @17:00:34.54
CSV.dial :flush @17:00:35.02 CS.seek 3ml @17:00:42.50 delay 5 seconds @17:00:44.29 delay 3 seconds @17:00:47.38 AS.reconfigure ASslow1 @17:00:47.45 AS.seek 0.684ml @17:00:47.53
CSV.dial :air @17:00:48.07 CS.seek 5ml @17:00:53.35 CSV.dial :air @17:00:53.38 CS.seek 0ml @17:01:04.87 delay 7 seconds @17:01:11.91 CS.seek 3ml @17:01:19.28 CSV.dial :waste @17:01:20.04 CS.seek 0ml @17:01:24.74 delay 3 seconds @17:01:27.34
Disinfecting external Intake with mfbkill @17:01:27.50 CSV.dial CSR @17:01:27.76 Eluate primed @17:01:27.77 ATV.dial :waste2 @17:01:28.39 AS.reconfigure ASnorm @17:01:28.46 AS.seek 0ml @17:01:28.96
Pulling 8ml of CSR.mfbkill @17:01:28.97 CSR.select :mfbkill @17:01:29.14 CS.seek 8ml @17:01:46.84 delay 5 seconds @17:01:51.87 CSR.select :air @17:01:52.01 CS.seek 10ml @17:01:57.29 CSV.dial CBV @17:01:57.33
CBV.dial CSV @17:01:58.33 CTV.dial :intake @17:01:59.08 Intake.select :open @17:01:59.22 CS.seek 2ml @17:02:03.28 SPE.coast @17:02:03.39 ASV.dial :SPErecovery @17:02:03.42 Setting up PCR for OM -- writing /var/log/esp/OM20may1417h450ml.pcr @17:02:03.46 Building PCR Slug from ASV with MM3 mix, PP5 primer @17:02:03.50 Priming with carrier @17:02:03.52 ATV.dial ARV @17:02:04.51 ARV.dial :carrier @17:02:05.44 AS.seek 0.5ml @17:02:17.91
Intake.select :close @17:02:18.05 CTV.dial :air @17:02:18.81 CS.reconfigure CSconfig @17:02:18.88 CS.seek 0ml @17:02:25.03 Flushing lower manifold's waste1 port @17:02:25.05 CSV.dial CSR @17:02:26.13 Pulling 4ml of CSR.mfbkill @17:02:26.14 CSR.select :mfbkill @17:02:26.31 CS.seek 4ml @17:02:30.99 AFV.select :carrierSolution @17:02:31.11 delay 2 seconds @17:02:33.14 AS.reconfigure ASconfig @17:02:33.22 AS.seek 1ml @17:02:37.50
delay 5 seconds @17:02:42.53 CSR.select :air @17:02:42.69 CS.reconfigure CSfast @17:02:42.76 CS.seek 10ml @17:02:56.06 CSV.dial CTV @17:02:56.10
CTV.dial CSV @17:02:57.47 CBV.dial :waste1 @17:02:57.98 CS.seek 0ml @17:03:02.26 delay 1 second @17:03:03.29 AFV.select :holdingCoil @17:03:03.42 delay 1 second @17:03:04.45 ATV.dial :waste @17:03:05.88 AS.reconfigure ASnorm @17:03:05.96 AS.seek :empty @17:03:19.50
Flushing lower manifold's waste2b port @17:03:19.52 CSV.dial CSR @17:03:20.07 Pulling 4ml of CSR.mfbkill @17:03:20.08 CSR.select :mfbkill @17:03:20.24 CS.reconfigure CSconfig @17:03:20.31 CS.seek 4ml @17:03:31.56 delay 5 seconds @17:03:36.59 CSR.select :air @17:03:36.74 CS.reconfigure CSfast @17:03:36.81 CS.seek 10ml @17:03:50.19 CSV.dial CTV @17:03:50.23
CTV.dial CSV @17:03:50.74 CBV.dial :waste2b @17:03:51.54 CS.seek 0ml @17:03:56.69 AFV.select :carrierSolution @17:03:56.82 delay 2 seconds @17:03:58.85 AS.reconfigure ASconfig @17:03:58.93 AS.seek 0.5ml @17:04:12.97
Killing upper manifold @17:04:12.99 CSV.dial CSR @17:04:13.56 Pulling 4ml of CSR.mfbkill @17:04:13.58 CSR.select :mfbkill @17:04:13.73 CS.reconfigure CSconfig @17:04:13.80 CS.seek 4ml @17:04:25.28 delay 5 seconds @17:04:28.04 delay 1 second @17:04:29.07 AFV.select :holdingCoil @17:04:29.20 Building Slug @17:04:29.21 ATV.dial :ARV @17:04:30.31
CSR.select :air @17:04:30.46 CS.reconfigure CSfast @17:04:30.53 CS.seek 10ml @17:04:30.79 delay 2 seconds @17:04:32.82 ARV.dial :air1 @17:04:33.21 delay 2 seconds @17:04:35.30 AS.reconfigure ASslow2 @17:04:35.39 AS.seek 0.506ml @17:04:41.60 delay 2 seconds @17:04:43.63 ARV.dial :MM3 @17:04:43.82 CSV.dial CBV @17:04:43.86
CBV.dial CSV @17:04:44.66 delay 2 seconds @17:04:44.89
CTV.dial :air @17:04:46.17 CS.seek 0ml @17:04:46.68 Adding MM3 @17:04:46.80 AS.seek 0.524ml @17:05:05.02 delay 25 seconds @17:05:07.54
delay 7 seconds @17:05:14.58 CS.seek 3ml @17:05:22.04 CSV.dial :waste @17:05:22.51 CS.seek 0ml @17:05:29.94 Flushing upper manifold @17:05:30.08 ARV.dial :PP5 @17:05:30.17
CSV.dial :flush @17:05:30.50 Adding PP5 @17:05:30.52 delay 2 seconds @17:05:30.65
CS.seek 5ml @17:05:32.61 AS.seek 0.53ml @17:05:38.91 delay 10 seconds @17:05:42.50
delay 5 seconds @17:05:47.53 CSV.dial :air @17:05:48.06 CS.seek 7ml @17:05:48.93 ARV.dial :air3 @17:05:49.32 adding ARV air @17:05:49.34 delay 2 seconds @17:05:51.44 AS.seek 0.536ml @17:05:53.40 CSV.dial CBV @17:05:53.44
CBV.dial CSV @17:05:54.44 CTV.dial :air @17:05:54.47 CS.seek 0ml @17:05:57.68 delay 5 seconds @17:06:02.71 ARV.dial :carrier @17:06:03.93 delay 7 seconds @17:06:09.79
delay 7 seconds @17:06:11.02 AS.seek 0.594ml @17:06:16.83
CS.seek 3ml @17:06:24.07 CSV.dial :waste @17:06:24.55 CS.seek 0ml @17:06:31.84 CSV.dial :flush @17:06:32.31 CS.seek 3ml @17:06:39.63 delay 5 seconds @17:06:44.66 CSV.dial :air @17:06:45.18 CS.seek 5.001ml @17:06:50.42 CSV.dial :air @17:06:50.45 CS.seek 0ml @17:07:01.82 delay 7 seconds @17:07:08.86 CS.seek 3ml @17:07:09.54 delay 5 seconds @17:07:14.57 ATV.dial :ASV @17:07:14.98 delay 2 seconds @17:07:16.10
CSV.dial :waste @17:07:16.84 CS.seek 0ml @17:07:17.07 AS.seek 0.6ml @17:07:23.31 delay 4 seconds @17:07:24.16
Flushing lower manifold's waste1 port @17:07:24.32 CSV.dial :flush @17:07:24.80 CS.seek 5ml @17:07:27.34 ATV.dial ARV @17:07:28.01 delay 2 seconds @17:07:30.04 ARV.dial :carrier @17:07:30.06 delay 2 seconds @17:07:32.17 AS.seek 0.65ml @17:07:36.04
delay 5 seconds @17:07:41.07 CSV.dial :air @17:07:41.72 CS.seek 7ml @17:07:46.98 CSV.dial CTV @17:07:47.02
CTV.dial CSV @17:07:48.38 CBV.dial :waste1 @17:07:48.88 CS.seek 0ml @17:08:04.19 Flushing lower manifold's waste2b port @17:08:04.35 CSV.dial :flush @17:08:05.09 CS.seek 5ml @17:08:16.44 delay 5 seconds @17:08:21.47 CSV.dial :air @17:08:22.01 CS.seek 7ml @17:08:22.87 delay 4 seconds @17:08:26.89 ATV.dial PCR @17:08:27.23 CSV.dial CTV @17:08:27.27
CTV.dial CSV @17:08:27.51 ASV.dial :waste1 @17:08:27.73
CBV.dial :waste2b @17:08:28.29 ARV.dial :air1 @17:08:28.52
CS.seek 0ml @17:08:28.70 Positioning Slug by 0.324ml @17:08:28.82 AS.seek 0.326ml @17:08:43.82
Disinfecting external Intake with kill @17:08:44.56 CSV.dial CSR @17:08:45.11 Pulling 8ml of CSR.kill @17:08:45.12 CSR.select :kill @17:08:45.28 CS.seek 8ml @17:09:02.57 delay 5 seconds @17:09:07.60 CSR.select :air @17:09:07.76 CS.seek 10ml @17:09:13.02 CSV.dial CBV @17:09:13.06
CBV.dial CSV @17:09:14.12 CTV.dial :intake @17:09:14.87 Intake.select :open @17:09:15.02 CS.seek 2ml @17:09:33.82 Intake.select :close @17:09:33.96 CTV.dial :air @17:09:34.70 CS.reconfigure CSconfig @17:09:34.77 CS.seek 0ml @17:09:40.88 CSV.dial CSR @17:09:41.94 Pulling 5ml of CSR.flush @17:09:41.96 CSR.select :flush @17:09:42.12 CS.seek 5ml @17:09:55.91 delay 5 seconds @17:10:00.94 CSR.select :air @17:10:01.09 CS.seek 10ml @17:10:14.82 CSV.dial CBV @17:10:15.83 CS.seek 0ml @17:10:42.32 Garaging FlushPuck in Collection Clamp @17:10:42.43 Elbow.reconfigure TightElbow @17:10:42.50 Elbow.seek CC @17:10:42.77 Elbow.reconfigure LooseElbow @17:10:42.90 Forearm.seek CC @17:10:43.92 Hand.close @17:10:44.58 CC.seek :open @17:10:49.01 Forearm.seek :retract @17:10:50.03 Elbow.reconfigure TightElbow @17:10:50.11 Elbow.seek :garage @17:10:53.28 Elbow.reconfigure LooseElbow @17:10:53.41 Forearm.reconfigure TightForearm @17:10:53.49 Forearm.seek :garage @17:10:54.65 Hand.open @17:10:55.40 Forearm.reconfigure LooseForearm @17:10:55.48 Forearm.seek :retract @17:10:56.54 Starting initialPurge @17:10:56.56 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:10:56.67 Hand.open @17:10:57.02 Elbow.reconfigure TightElbow @17:10:57.09 Elbow.seek FlushPuck::Garage @17:10:57.25 Elbow.reconfigure LooseElbow @17:10:57.38 Forearm.seek :garage @17:10:58.42 Hand.close @17:10:59.21 Forearm.seek :retract @17:11:00.29 Elbow.reconfigure TightElbow @17:11:00.36 Elbow.seek CC @17:11:03.28 Elbow.reconfigure LooseElbow @17:11:03.42 Forearm.reconfigure TightForearm @17:11:03.49 Forearm.seek CC @17:11:04.86 CC.seek :closed @17:11:09.13 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.66 @17:11:09.82 Hand.open @17:11:10.52 Forearm.reconfigure LooseForearm @17:11:10.59 Forearm.seek :retract @17:11:11.61 Flushing the Collection Valve::Manifold @17:11:11.77 CSV.dial CSR @17:11:12.84 Pulling 5ml of CSR.flush @17:11:12.85 CSR.select :flush @17:11:13.02 CS.seek 5ml @17:11:26.89 delay 5 seconds @17:11:31.92 CSR.select :air @17:11:32.12 CS.reconfigure CSfast @17:11:32.20 CS.seek 7.501ml @17:11:38.51 CSV.dial :waste @17:11:39.78 CS.reconfigure CSconfig @17:11:39.86 CS.seek 0ml @17:12:00.04 Purging external Intake @17:12:00.20 CSV.dial :flush @17:12:00.67 CS.reconfigure CSfast @17:12:00.75 CS.seek 10ml @17:12:22.17 delay 5 seconds @17:12:27.20 CSV.dial CBV @17:12:27.25
CBV.dial CSV @17:12:27.98 CTV.dial :intake @17:12:28.73 Intake.select :open @17:12:28.87 CS.seek 0ml @17:12:51.54 Intake.select :close @17:12:51.67 CTV.dial :air @17:12:52.42 Flushing upper manifold @17:12:52.57 CSV.dial :flush @17:12:53.32 CS.seek 5ml @17:13:04.57 delay 5 seconds @17:13:09.60 CSV.dial :air @17:13:10.13 CS.seek 7ml @17:13:15.43 CSV.dial CBV @17:13:15.45
CBV.dial CSV @17:13:16.48 CTV.dial :air @17:13:16.51 CS.seek 0ml @17:13:31.76 delay 7 seconds @17:13:38.80 CS.seek 3ml @17:13:46.04 CSV.dial :waste @17:13:46.54 CS.seek 0ml @17:13:53.91 CSV.dial :flush @17:13:54.37 CS.seek 3ml @17:13:54.78 delay 5 seconds @17:13:59.80 ATV.dial ARV @17:14:00.56 ASV.dial :PCRlock @17:14:00.95 delay 5 seconds @17:14:01.64
delay 5 seconds @17:14:05.98 Running OM Profile60C350 thermocycling @17:14:06.00 PCR.program Profile60C350 @17:14:06.14 Profile60C350 should complete in < 2:02:45 @17:14:06.16 PCR.run 2:38:26.25 @17:14:06.66
CSV.dial :air @17:14:07.19 CS.seek 5ml @17:14:12.39 CSV.dial :air @17:14:12.41 CS.seek 0ml @17:14:24.04 delay 7 seconds @17:14:31.07 CS.seek 3ml @17:14:38.39 CSV.dial :waste @17:14:39.15 CS.seek 0ml @17:14:46.42 Garaging FlushPuck in Collection Clamp @17:14:46.53 Elbow.reconfigure TightElbow @17:14:46.59 Elbow.seek CC @17:14:46.85 Elbow.reconfigure LooseElbow @17:14:46.98 Forearm.seek CC @17:14:48.00 Hand.close @17:14:48.58 CC.seek :open @17:14:52.96 Forearm.seek :retract @17:14:53.99 Elbow.reconfigure TightElbow @17:14:54.06 Elbow.seek :garage @17:14:57.22 Elbow.reconfigure LooseElbow @17:14:57.94 Forearm.reconfigure TightForearm @17:14:58.02 Forearm.seek :garage @17:14:59.16 Hand.open @17:14:59.90 Forearm.reconfigure LooseForearm @17:14:59.97 Forearm.seek :retract @17:15:00.99 Completed [stdphase] begun 14:54:45 @17:15:01.00 Preparing to sleep until 10:00 15-may-20 @17:15:01.08 deferred until MFBPCR finish @17:15:01.11
Waiting for MFBPCR til 10:00 15-may-20 @17:15:01.17 Continuing at 10:00CEST15-May-20 @17:15:01.25 GoodNews.email "Then, in tube 2: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:119\n{1 => 6, 2 => 16}\nCan@17:15:01, 10.6C, 43% humidity, 14.5psia, 14.876V, 0.916A, 0.916A avg, 13.62W",Subject: "Waiting until 10:00CEST15-May-20" @18:09:49.99 -> exit @18:09:50.18 Session Ended @18:35:28.72 Session Begun @18:35:29.84 Session Ended @18:36:01.25 Session Begun @18:36:05.59 -> showlog 10 @18:50:36.26 PCR.off @18:50:37.55 PCR for OM completed -- closed /var/log/esp/OM20may1417h450ml.pcr @18:50:37.65 Cleaning PCR @18:50:37.66 AFV.select :carrierSolution @18:50:37.79 delay 2 seconds @18:50:39.87 AS.reconfigure ASconfig @18:50:39.94 AS.seek 0.5ml @18:50:50.26 delay 1 second @18:50:51.28 AFV.select :holdingCoil @18:50:51.40 ASV.dial :waste1 @18:50:51.80 ATV.dial :air3 @18:50:53.62 AS.reconfigure ASslow1 @18:50:53.69 AS.seek 0.51ml @18:50:56.01 delay 2 seconds @18:50:58.03 ATV.dial :bleach @18:50:58.65 AS.seek 0.81ml @18:51:59.26 delay 3 seconds @18:52:02.29 ATV.dial :air3 @18:52:02.85 delay 3 seconds @18:52:05.94 AS.seek 0.835ml @18:52:11.25 delay 3 seconds @18:52:14.28 ATV.dial :bleach @18:52:14.90 AS.seek 0.82ml @18:52:18.24 delay 3 seconds @18:52:21.27 ARV.dial :waste @18:52:21.87 delay 3 seconds @18:52:24.90 ATV.dial :ARV @18:52:25.76 AS.reconfigure ASconfig @18:52:25.83 AS.seek 0.47ml @18:52:46.26 AFV.select :carrierSolution @18:52:46.39 delay 2 seconds @18:52:48.43 AS.seek 1ml @18:53:19.24 delay 1 second @18:53:20.27 AFV.select :holdingCoil @18:53:20.39 delay 1 second @18:53:21.42 AS.reconfigure ASfast @18:53:21.49 AS.seek 0ml @18:53:48.87 AFV.select :carrierSolution @18:53:49.00 delay 2 seconds @18:53:51.03 AS.reconfigure ASconfig @18:53:51.10 AS.seek 1ml @18:54:48.93 delay 1 second @18:54:49.95 AFV.select :holdingCoil @18:54:50.07 delay 1 second @18:54:51.10 AS.reconfigure ASfast @18:54:51.17 AS.seek 0ml @18:55:18.54 AFV.select :carrierSolution @18:55:18.67 delay 2 seconds @18:55:20.70 AS.reconfigure ASconfig @18:55:20.77 AS.seek 1ml @18:56:18.54 delay 1 second @18:56:19.56 AFV.select :holdingCoil @18:56:19.68 delay 1 second @18:56:20.71 AS.reconfigure ASfast @18:56:20.78 AS.seek 0ml @18:56:48.13 ARV.dial between carrier and air1 @18:56:48.63 ATV.dial :air3 @18:56:49.88 AS.reconfigure ASslow1 @18:56:49.95 AS.seek 0.01ml @18:56:52.37 delay 2 seconds @18:56:54.39 ATV.dial :bleach @18:56:55.02 AS.seek 0.81ml @18:59:36.25 delay 3 seconds @18:59:39.28 ATV.dial :air3 @18:59:39.84 delay 3 seconds @18:59:42.93 AS.seek 0.835ml @18:59:48.26 delay 3 seconds @18:59:51.29 ATV.dial :bleach @18:59:51.91 AS.seek 0.82ml @18:59:55.25 delay 3 seconds @18:59:58.28 ATV.dial PCR @18:59:58.70 AS.reconfigure ASconfig @18:59:58.77 AS.seek :empty @19:00:46.25 PCR.program :bleachProfile @19:00:46.32 PCR program should complete in < 2:45 @19:00:46.33 PCR.run 10:00 @19:04:00.28 PCR.off @19:04:00.33 AFV.select :carrierSolution @19:04:00.45 delay 2 seconds @19:04:02.49 AS.seek 1ml @19:05:00.39 delay 1 second @19:05:01.41 AFV.select :holdingCoil @19:05:01.53 delay 1 second @19:05:02.56 AS.reconfigure ASfast @19:05:02.63 AS.seek 0ml @19:05:29.98 delay 3 seconds @19:05:33.01 AFV.select :carrierSolution @19:05:33.13 delay 2 seconds @19:05:35.16 AS.reconfigure ASconfig @19:05:35.23 AS.seek 1ml @19:06:33.03 delay 1 second @19:06:34.05 AFV.select :holdingCoil @19:06:34.17 delay 1 second @19:06:35.20 AS.reconfigure ASfast @19:06:35.27 AS.seek 0ml @19:07:02.61 delay 3 seconds @19:07:05.64 AFV.select :carrierSolution @19:07:05.76 delay 2 seconds @19:07:08.29 AS.reconfigure ASconfig @19:07:08.45 AS.seek 1ml @19:07:42.47 Session Ended @19:08:06.28 delay 1 second @19:08:07.30 AFV.select :holdingCoil @19:08:07.40 delay 1 second @19:08:08.43 AS.reconfigure ASfast @19:08:08.48 AS.seek 0ml @19:08:35.83 delay 3 seconds @19:08:38.86 AFV.select :carrierSolution @19:08:38.96 delay 2 seconds @19:08:40.99 AS.reconfigure ASconfig @19:08:41.05 AS.seek 1ml @19:09:38.89 delay 1 second @19:09:39.91 AFV.select :holdingCoil @19:09:40.01 delay 1 second @19:09:41.04 AS.reconfigure ASfast @19:09:41.09 AS.seek 0ml @19:10:08.42 delay 3 seconds @19:10:11.45 AFV.select :carrierSolution @19:10:11.55 delay 2 seconds @19:10:13.58 AS.reconfigure ASconfig @19:10:13.64 AS.seek 1ml @19:11:11.44 delay 1 second @19:11:12.46 AFV.select :holdingCoil @19:11:12.56 delay 1 second @19:11:13.59 AS.reconfigure ASnorm @19:11:13.64 AS.seek 0ml @19:12:04.37 Setting up PCR for ML -- writing /var/log/esp/ML20may1419h450ml.pcr @19:12:04.39 Building PCR Slug from ASV with MM2 mix, PP4 primer @19:12:04.41 Priming with carrier @19:12:04.42 ATV.dial ARV @19:12:05.00 ARV.dial :carrier @19:12:05.34 AS.seek 0.5ml @19:12:30.91 AFV.select :carrierSolution @19:12:31.02 delay 2 seconds @19:12:33.05 AS.reconfigure ASconfig @19:12:33.10 AS.seek 1ml @19:13:02.18 delay 1 second @19:13:03.20 AFV.select :holdingCoil @19:13:03.30 delay 1 second @19:13:04.33 ATV.dial :waste @19:13:05.89 AS.reconfigure ASnorm @19:13:05.94 AS.seek :empty @19:13:56.68 AFV.select :carrierSolution @19:13:56.79 delay 2 seconds @19:13:58.82 AS.reconfigure ASconfig @19:13:58.87 AS.seek 0.5ml @19:14:28.02 delay 1 second @19:14:29.04 AFV.select :holdingCoil @19:14:29.14 Building Slug @19:14:29.15 ATV.dial :ARV @19:14:30.69 delay 2 seconds @19:14:32.72 ARV.dial :air1 @19:14:33.11 delay 2 seconds @19:14:35.20 AS.reconfigure ASslow2 @19:14:35.25 AS.seek 0.506ml @19:14:41.41 delay 2 seconds @19:14:43.44 ARV.dial :MM2 @19:14:44.83 delay 2 seconds @19:14:46.86 Adding MM2 @19:14:46.94 AS.seek 0.524ml @19:15:05.21 delay 25 seconds @19:15:30.24 ARV.dial :PP4 @19:15:30.61 Adding PP4 @19:15:30.63 delay 2 seconds @19:15:32.71 AS.seek 0.53ml @19:15:38.97 delay 10 seconds @19:15:49.00 ARV.dial :air3 @19:15:49.38 adding ARV air @19:15:49.40 delay 2 seconds @19:15:51.48 AS.seek 0.536ml @19:15:57.83 delay 5 seconds @19:16:02.86 ARV.dial :carrier @19:16:04.05 delay 7 seconds @19:16:11.14 AS.seek 0.594ml @19:17:10.02 delay 5 seconds @19:17:15.05 ATV.dial :ASV @19:17:15.44 delay 2 seconds @19:17:17.53 AS.seek 0.6ml @19:17:23.76 delay 4 seconds @19:17:27.79 ATV.dial ARV @19:17:28.33 delay 2 seconds @19:17:30.36 ARV.dial :carrier @19:17:30.37 delay 2 seconds @19:17:32.46 AS.seek 0.65ml @19:18:22.96 delay 4 seconds @19:18:26.99 ATV.dial PCR @19:18:27.57 ASV.dial :waste1 @19:18:27.58 ARV.dial :air1 @19:18:27.97 Positioning Slug by 0.324ml @19:18:28.06 AS.seek 0.326ml @19:23:54.07 delay 5 seconds @19:23:59.10 ATV.dial ARV @19:23:59.84 ASV.dial :PCRlock @19:24:00.23 delay 5 seconds @19:24:05.26 Running ML Profile60C350 thermocycling @19:24:05.28 PCR.program Profile60C350 @19:24:05.38 Profile60C350 should complete in < 2:02:45 @19:24:05.40 PCR.run 2:38:26.25 @21:00:14.81 PCR.off @21:00:16.08 PCR for ML completed -- closed /var/log/esp/ML20may1419h450ml.pcr @21:00:16.15 Cleaning PCR @21:00:16.16 AFV.select :carrierSolution @21:00:16.27 delay 2 seconds @21:00:18.30 AS.reconfigure ASconfig @21:00:18.35 AS.seek 0.5ml @21:00:28.68 delay 1 second @21:00:29.70 AFV.select :holdingCoil @21:00:29.80 ASV.dial :waste1 @21:00:30.18 ATV.dial :air3 @21:00:31.39 AS.reconfigure ASslow1 @21:00:31.44 AS.seek 0.51ml @21:00:33.77 delay 2 seconds @21:00:35.79 ATV.dial :bleach @21:00:36.39 AS.seek 0.81ml @21:01:36.98 delay 3 seconds @21:01:40.00 ATV.dial :air3 @21:01:40.54 delay 3 seconds @21:01:43.63 AS.seek 0.835ml @21:01:48.96 delay 3 seconds @21:01:51.99 ATV.dial :bleach @21:01:52.60 AS.seek 0.82ml @21:01:55.87 delay 3 seconds @21:01:58.89 ARV.dial :waste @21:01:59.48 delay 3 seconds @21:02:02.51 ATV.dial :ARV @21:02:03.46 AS.reconfigure ASconfig @21:02:03.52 AS.seek 0.47ml @21:02:23.92 AFV.select :carrierSolution @21:02:24.03 delay 2 seconds @21:02:26.07 AS.seek 1ml @21:02:56.86 delay 1 second @21:02:57.88 AFV.select :holdingCoil @21:02:57.98 delay 1 second @21:02:59.01 AS.reconfigure ASfast @21:02:59.06 AS.seek 0ml @21:03:26.40 AFV.select :carrierSolution @21:03:26.51 delay 2 seconds @21:03:28.54 AS.reconfigure ASconfig @21:03:28.59 AS.seek 1ml @21:04:26.33 delay 1 second @21:04:27.35 AFV.select :holdingCoil @21:04:27.45 delay 1 second @21:04:28.48 AS.reconfigure ASfast @21:04:28.53 AS.seek 0ml @21:04:55.87 AFV.select :carrierSolution @21:04:55.98 delay 2 seconds @21:04:58.01 AS.reconfigure ASconfig @21:04:58.64 AS.seek 1ml @21:05:56.44 delay 1 second @21:05:57.46 AFV.select :holdingCoil @21:05:57.56 delay 1 second @21:05:58.59 AS.reconfigure ASfast @21:05:58.64 AS.seek 0ml @21:06:26.00 ARV.dial between carrier and air1 @21:06:26.48 ATV.dial :air3 @21:06:27.71 AS.reconfigure ASslow1 @21:06:27.76 AS.seek 0.01ml @21:06:30.09 delay 2 seconds @21:06:32.11 ATV.dial :bleach @21:06:32.71 AS.seek 0.81ml @21:09:13.83 delay 3 seconds @21:09:16.86 ATV.dial :air3 @21:09:17.39 delay 3 seconds @21:09:20.48 AS.seek 0.835ml @21:09:25.76 delay 3 seconds @21:09:28.79 ATV.dial :bleach @21:09:29.40 AS.seek 0.82ml @21:09:32.72 delay 3 seconds @21:09:35.75 ATV.dial PCR @21:09:36.14 AS.reconfigure ASconfig @21:09:36.19 AS.seek :empty @21:10:23.66 PCR.program :bleachProfile @21:10:23.72 PCR program should complete in < 2:45 @21:10:23.73 PCR.run 10:00 @21:13:37.88 PCR.off @21:13:37.94 AFV.select :carrierSolution @21:13:38.13 delay 2 seconds @21:13:40.21 AS.seek 1ml @21:14:38.15 delay 1 second @21:14:39.23 AFV.select :holdingCoil @21:14:39.39 delay 1 second @21:14:40.47 AS.reconfigure ASfast @21:14:40.61 AS.seek 0ml @21:15:07.99 delay 3 seconds @21:15:11.02 AFV.select :carrierSolution @21:15:11.12 delay 2 seconds @21:15:13.15 AS.reconfigure ASconfig @21:15:13.21 AS.seek 1ml @21:16:10.94 delay 1 second @21:16:11.96 AFV.select :holdingCoil @21:16:12.09 delay 1 second @21:16:13.12 AS.reconfigure ASfast @21:16:13.17 AS.seek 0ml @21:16:40.51 delay 3 seconds @21:16:43.54 AFV.select :carrierSolution @21:16:43.64 delay 2 seconds @21:16:45.67 AS.reconfigure ASconfig @21:16:45.73 AS.seek 1ml @21:17:43.52 delay 1 second @21:17:44.54 AFV.select :holdingCoil @21:17:44.64 delay 1 second @21:17:45.67 AS.reconfigure ASfast @21:17:45.72 AS.seek 0ml @21:18:13.04 delay 3 seconds @21:18:16.07 AFV.select :carrierSolution @21:18:16.17 delay 2 seconds @21:18:18.20 AS.reconfigure ASconfig @21:18:18.26 AS.seek 1ml @21:19:16.01 delay 1 second @21:19:17.03 AFV.select :holdingCoil @21:19:17.13 delay 1 second @21:19:18.16 AS.reconfigure ASfast @21:19:18.21 AS.seek 0ml @21:19:45.55 delay 3 seconds @21:19:48.58 AFV.select :carrierSolution @21:19:48.68 delay 2 seconds @21:19:50.71 AS.reconfigure ASconfig @21:19:50.77 AS.seek 1ml @21:20:48.52 delay 1 second @21:20:49.54 AFV.select :holdingCoil @21:20:49.64 delay 1 second @21:20:50.67 AS.reconfigure ASnorm @21:20:50.72 AS.seek 0ml @21:21:41.52 Setting up PCR for CG -- writing /var/log/esp/CG20may1421h450ml.pcr @21:21:41.56 Building PCR Slug from ASV with MM1 mix, PP2 primer @21:21:41.57 Priming with carrier @21:21:41.58 ATV.dial ARV @21:21:42.16 ARV.dial :carrier @21:21:42.50 AS.seek 0.5ml @21:22:08.01 AFV.select :carrierSolution @21:22:08.13 delay 2 seconds @21:22:10.16 AS.reconfigure ASconfig @21:22:10.21 AS.seek 1ml @21:22:39.23 delay 1 second @21:22:40.25 AFV.select :holdingCoil @21:22:40.35 delay 1 second @21:22:41.38 ATV.dial :waste @21:22:42.95 AS.reconfigure ASnorm @21:22:43.00 AS.seek :empty @21:23:33.76 AFV.select :carrierSolution @21:23:33.87 delay 2 seconds @21:23:35.90 AS.reconfigure ASconfig @21:23:35.95 AS.seek 0.5ml @21:24:05.03 delay 1 second @21:24:06.64 AFV.select :holdingCoil @21:24:06.74 Building Slug @21:24:06.75 ATV.dial :ARV @21:24:08.29 delay 2 seconds @21:24:10.32 ARV.dial :air1 @21:24:10.70 delay 2 seconds @21:24:12.79 AS.reconfigure ASslow2 @21:24:12.84 AS.seek 0.506ml @21:24:19.04 delay 2 seconds @21:24:21.07 ARV.dial :MM1 @21:24:21.64 delay 2 seconds @21:24:23.67 Adding MM1 @21:24:23.74 AS.seek 0.524ml @21:24:41.99 delay 25 seconds @21:25:07.02 ARV.dial :PP2 @21:25:07.40 Adding PP2 @21:25:07.42 delay 2 seconds @21:25:09.50 AS.seek 0.53ml @21:25:15.79 delay 10 seconds @21:25:25.82 ARV.dial :air2 @21:25:26.20 adding ARV air @21:25:26.22 delay 2 seconds @21:25:28.30 AS.seek 0.536ml @21:25:34.84 delay 5 seconds @21:25:39.87 ARV.dial :carrier @21:25:41.07 delay 7 seconds @21:25:48.16 AS.seek 0.594ml @21:26:46.70 delay 5 seconds @21:26:51.73 ATV.dial :ASV @21:26:52.12 delay 2 seconds @21:26:54.21 AS.seek 0.6ml @21:27:00.39 delay 4 seconds @21:27:04.42 ATV.dial ARV @21:27:04.95 delay 2 seconds @21:27:06.98 ARV.dial :carrier @21:27:06.99 delay 2 seconds @21:27:09.08 AS.seek 0.65ml @21:27:59.70 delay 4 seconds @21:28:03.73 ATV.dial PCR @21:28:04.31 ASV.dial :waste1 @21:28:04.32 ARV.dial :air1 @21:28:04.73 Positioning Slug by 0.324ml @21:28:04.82 AS.seek 0.326ml @21:33:30.80 delay 5 seconds @21:33:35.83 ATV.dial ARV @21:33:36.56 ASV.dial :PCRlock @21:33:36.94 delay 5 seconds @21:33:41.97 Running CG Profile60C300 thermocycling @21:33:41.99 PCR.program Profile60C300 @21:33:42.12 Profile60C300 should complete in < 1:59:25 @21:33:42.13 PCR.run 2:34:16.25 @23:09:08.10 PCR.off @23:09:09.40 PCR for CG completed -- closed /var/log/esp/CG20may1421h450ml.pcr @23:09:09.47 Cleaning PCR @23:09:09.48 AFV.select :carrierSolution @23:09:09.59 delay 2 seconds @23:09:11.62 AS.reconfigure ASconfig @23:09:11.67 AS.seek 0.5ml @23:09:21.99 delay 1 second @23:09:23.01 AFV.select :holdingCoil @23:09:23.11 ASV.dial :waste1 @23:09:23.50 ATV.dial :air3 @23:09:24.73 AS.reconfigure ASslow1 @23:09:24.78 AS.seek 0.51ml @23:09:27.07 delay 2 seconds @23:09:29.09 ATV.dial :bleach @23:09:29.70 AS.seek 0.81ml @23:10:30.30 delay 3 seconds @23:10:33.33 ATV.dial :air3 @23:10:33.86 delay 3 seconds @23:10:36.95 AS.seek 0.835ml @23:10:42.21 delay 3 seconds @23:10:45.24 ATV.dial :bleach @23:10:45.85 AS.seek 0.82ml @23:10:49.13 delay 3 seconds @23:10:52.15 ARV.dial :waste @23:10:52.74 delay 3 seconds @23:10:55.77 ATV.dial :ARV @23:10:56.62 AS.reconfigure ASconfig @23:10:56.67 AS.seek 0.47ml @23:11:17.05 AFV.select :carrierSolution @23:11:17.17 delay 2 seconds @23:11:19.21 AS.seek 1ml @23:11:49.96 delay 1 second @23:11:50.98 AFV.select :holdingCoil @23:11:51.08 delay 1 second @23:11:52.11 AS.reconfigure ASfast @23:11:52.16 AS.seek 0ml @23:12:19.50 AFV.select :carrierSolution @23:12:19.61 delay 2 seconds @23:12:21.64 AS.reconfigure ASconfig @23:12:21.69 AS.seek 1ml @23:13:19.47 delay 1 second @23:13:20.49 AFV.select :holdingCoil @23:13:20.59 delay 1 second @23:13:21.62 AS.reconfigure ASfast @23:13:21.67 AS.seek 0ml @23:13:49.02 AFV.select :carrierSolution @23:13:49.13 delay 2 seconds @23:13:51.16 AS.reconfigure ASconfig @23:13:51.21 AS.seek 1ml @23:14:48.97 delay 1 second @23:14:49.99 AFV.select :holdingCoil @23:14:50.09 delay 1 second @23:14:51.12 AS.reconfigure ASfast @23:14:51.17 AS.seek 0ml @23:15:18.52 ARV.dial between carrier and air1 @23:15:19.00 ATV.dial :air3 @23:15:20.22 AS.reconfigure ASslow1 @23:15:20.28 AS.seek 0.01ml @23:15:22.55 delay 2 seconds @23:15:24.57 ATV.dial :bleach @23:15:25.17 AS.seek 0.81ml @23:18:06.30 delay 3 seconds @23:18:09.33 ATV.dial :air3 @23:18:09.87 delay 3 seconds @23:18:12.96 AS.seek 0.835ml @23:18:18.27 delay 3 seconds @23:18:21.30 ATV.dial :bleach @23:18:21.91 AS.seek 0.82ml @23:18:25.19 delay 3 seconds @23:18:28.22 ATV.dial PCR @23:18:28.62 AS.reconfigure ASconfig @23:18:28.67 AS.seek :empty @23:19:16.13 PCR.program :bleachProfile @23:19:16.19 PCR program should complete in < 2:45 @23:19:16.20 PCR.run 10:00 @23:22:30.15 PCR.off @23:22:30.17 AFV.select :carrierSolution @23:22:30.28 delay 2 seconds @23:22:32.32 AS.seek 1ml @23:23:30.23 delay 1 second @23:23:31.25 AFV.select :holdingCoil @23:23:31.35 delay 1 second @23:23:32.38 AS.reconfigure ASfast @23:23:32.43 AS.seek 0ml @23:23:59.78 delay 3 seconds @23:24:02.80 AFV.select :carrierSolution @23:24:03.49 delay 2 seconds @23:24:05.52 AS.reconfigure ASconfig @23:24:05.57 AS.seek 1ml @23:25:03.36 delay 1 second @23:25:04.38 AFV.select :holdingCoil @23:25:04.48 delay 1 second @23:25:05.51 AS.reconfigure ASfast @23:25:05.56 AS.seek 0ml @23:25:32.92 delay 3 seconds @23:25:35.95 AFV.select :carrierSolution @23:25:36.05 delay 2 seconds @23:25:38.08 AS.reconfigure ASconfig @23:25:38.14 AS.seek 1ml @23:26:35.93 delay 1 second @23:26:36.95 AFV.select :holdingCoil @23:26:37.05 delay 1 second @23:26:38.08 AS.reconfigure ASfast @23:26:38.13 AS.seek 0ml @23:27:05.43 delay 3 seconds @23:27:08.46 AFV.select :carrierSolution @23:27:08.56 delay 2 seconds @23:27:10.59 AS.reconfigure ASconfig @23:27:10.65 AS.seek 1ml @23:28:08.39 delay 1 second @23:28:09.41 AFV.select :holdingCoil @23:28:09.51 delay 1 second @23:28:10.54 AS.reconfigure ASfast @23:28:10.59 AS.seek 0ml @23:28:37.94 delay 3 seconds @23:28:40.97 AFV.select :carrierSolution @23:28:41.07 delay 2 seconds @23:28:43.10 AS.reconfigure ASconfig @23:28:43.16 AS.seek 1ml @23:29:40.92 delay 1 second @23:29:41.95 AFV.select :holdingCoil @23:29:42.05 delay 1 second @23:29:43.08 AS.reconfigure ASnorm @23:29:43.13 AS.seek 0ml @23:30:33.88 Setting up PCR for NM -- writing /var/log/esp/NM20may1423h450ml.pcr @23:30:33.90 Building PCR Slug from ASV with MM1 mix, PP1 primer @23:30:33.91 Priming with carrier @23:30:33.92 ATV.dial ARV @23:30:34.50 ARV.dial :carrier @23:30:34.85 AS.seek 0.5ml @23:31:00.44 AFV.select :carrierSolution @23:31:00.55 delay 2 seconds @23:31:02.58 AS.reconfigure ASconfig @23:31:02.63 AS.seek 1ml @23:31:31.64 delay 1 second @23:31:32.66 AFV.select :holdingCoil @23:31:32.76 delay 1 second @23:31:33.79 ATV.dial :waste @23:31:35.36 AS.reconfigure ASnorm @23:31:35.41 AS.seek :empty @23:32:26.17 AFV.select :carrierSolution @23:32:26.28 delay 2 seconds @23:32:28.31 AS.reconfigure ASconfig @23:32:28.36 AS.seek 0.5ml @23:32:57.44 delay 1 second @23:32:58.46 AFV.select :holdingCoil @23:32:58.56 Building Slug @23:32:58.57 ATV.dial :ARV @23:33:00.11 delay 2 seconds @23:33:02.14 ARV.dial :air1 @23:33:02.53 delay 2 seconds @23:33:04.62 AS.reconfigure ASslow2 @23:33:04.67 AS.seek 0.506ml @23:33:10.91 delay 2 seconds @23:33:12.93 ARV.dial :MM1 @23:33:13.50 delay 2 seconds @23:33:15.53 Adding MM1 @23:33:15.60 AS.seek 0.524ml @23:33:33.89 delay 25 seconds @23:33:58.92 ARV.dial :PP1 @23:33:59.30 Adding PP1 @23:33:59.31 delay 2 seconds @23:34:01.42 AS.seek 0.53ml @23:34:07.58 delay 10 seconds @23:34:17.61 ARV.dial :air1 @23:34:17.97 adding ARV air @23:34:17.99 delay 2 seconds @23:34:20.07 AS.seek 0.536ml @23:34:26.25 delay 5 seconds @23:34:31.28 ARV.dial :carrier @23:34:31.67 delay 7 seconds @23:34:38.76 AS.seek 0.594ml @23:35:37.30 delay 5 seconds @23:35:42.33 ATV.dial :ASV @23:35:42.72 delay 2 seconds @23:35:44.81 AS.seek 0.6ml @23:35:51.03 delay 4 seconds @23:35:55.06 ATV.dial ARV @23:35:55.58 delay 2 seconds @23:35:57.61 ARV.dial :carrier @23:35:57.62 delay 2 seconds @23:35:59.72 AS.seek 0.65ml @23:36:50.39 delay 4 seconds @23:36:54.42 ATV.dial PCR @23:36:55.00 ASV.dial :waste1 @23:36:55.01 ARV.dial :air1 @23:36:55.40 Positioning Slug by 0.324ml @23:36:55.49 AS.seek 0.326ml @23:42:21.54 delay 5 seconds @23:42:26.57 ATV.dial ARV @23:42:27.88 ASV.dial :PCRlock @23:42:28.27 delay 5 seconds @23:42:33.30 Running NM Profile60C300 thermocycling @23:42:33.31 PCR.program Profile60C300 @23:42:33.41 Profile60C300 should complete in < 1:59:25 @23:42:33.43 PCR.run 2:34:16.25 @01:18:02.77CEST15-May-20 PCR.off @01:18:03.45 PCR for NM completed -- closed /var/log/esp/NM20may1423h450ml.pcr @01:18:03.52 Cleaning PCR @01:18:03.53 AFV.select :carrierSolution @01:18:03.64 delay 2 seconds @01:18:05.67 AS.reconfigure ASconfig @01:18:05.72 AS.seek 0.5ml @01:18:16.03 delay 1 second @01:18:17.05 AFV.select :holdingCoil @01:18:17.15 ASV.dial :waste1 @01:18:17.53 ATV.dial :air3 @01:18:18.76 AS.reconfigure ASslow1 @01:18:18.81 AS.seek 0.51ml @01:18:21.12 delay 2 seconds @01:18:23.14 ATV.dial :bleach @01:18:23.74 AS.seek 0.81ml @01:19:24.31 delay 3 seconds @01:19:27.34 ATV.dial :air3 @01:19:27.86 delay 3 seconds @01:19:30.95 AS.seek 0.835ml @01:19:36.24 delay 3 seconds @01:19:39.26 ATV.dial :bleach @01:19:39.87 AS.seek 0.82ml @01:19:43.20 delay 3 seconds @01:19:46.23 ARV.dial :waste @01:19:46.81 delay 3 seconds @01:19:49.84 ATV.dial :ARV @01:19:50.68 AS.reconfigure ASconfig @01:19:50.74 AS.seek 0.47ml @01:20:11.16 AFV.select :carrierSolution @01:20:11.27 delay 2 seconds @01:20:13.31 AS.seek 1ml @01:20:44.03 delay 1 second @01:20:45.05 AFV.select :holdingCoil @01:20:45.15 delay 1 second @01:20:46.18 AS.reconfigure ASfast @01:20:46.23 AS.seek 0ml @01:21:13.55 AFV.select :carrierSolution @01:21:13.66 delay 2 seconds @01:21:15.69 AS.reconfigure ASconfig @01:21:15.74 AS.seek 1ml @01:22:13.53 delay 1 second @01:22:14.55 AFV.select :holdingCoil @01:22:14.65 delay 1 second @01:22:15.68 AS.reconfigure ASfast @01:22:15.73 AS.seek 0ml @01:22:43.06 AFV.select :carrierSolution @01:22:43.17 delay 2 seconds @01:22:45.20 AS.reconfigure ASconfig @01:22:45.25 AS.seek 1ml @01:23:43.00 delay 1 second @01:23:44.02 AFV.select :holdingCoil @01:23:44.12 delay 1 second @01:23:45.15 AS.reconfigure ASfast @01:23:45.20 AS.seek 0ml @01:24:13.11 ARV.dial between carrier and air1 @01:24:13.60 ATV.dial :air3 @01:24:14.84 AS.reconfigure ASslow1 @01:24:14.89 AS.seek 0.01ml @01:24:17.21 delay 2 seconds @01:24:19.23 ATV.dial :bleach @01:24:19.84 AS.seek 0.81ml @01:27:00.96 delay 3 seconds @01:27:03.99 ATV.dial :air3 @01:27:04.52 delay 3 seconds @01:27:07.61 AS.seek 0.835ml @01:27:12.86 delay 3 seconds @01:27:15.89 ATV.dial :bleach @01:27:16.50 AS.seek 0.82ml @01:27:19.80 delay 3 seconds @01:27:22.83 ATV.dial PCR @01:27:23.22 AS.reconfigure ASconfig @01:27:23.27 AS.seek :empty @01:28:10.71 PCR.program :bleachProfile @01:28:10.77 PCR program should complete in < 2:45 @01:28:10.78 PCR.run 10:00 @01:31:24.72 PCR.off @01:31:24.74 AFV.select :carrierSolution @01:31:24.85 delay 2 seconds @01:31:26.89 AS.seek 1ml @01:32:24.71 delay 1 second @01:32:25.73 AFV.select :holdingCoil @01:32:25.83 delay 1 second @01:32:26.86 AS.reconfigure ASfast @01:32:26.91 AS.seek 0ml @01:32:54.24 delay 3 seconds @01:32:57.27 AFV.select :carrierSolution @01:32:57.37 delay 2 seconds @01:32:59.41 AS.reconfigure ASconfig @01:32:59.46 AS.seek 1ml @01:33:57.26 delay 1 second @01:33:58.28 AFV.select :holdingCoil @01:33:58.38 delay 1 second @01:33:59.41 AS.reconfigure ASfast @01:33:59.46 AS.seek 0ml @01:34:26.82 delay 3 seconds @01:34:29.85 AFV.select :carrierSolution @01:34:29.95 delay 2 seconds @01:34:31.98 AS.reconfigure ASconfig @01:34:32.03 AS.seek 1ml @01:35:29.79 delay 1 second @01:35:30.81 AFV.select :holdingCoil @01:35:30.91 delay 1 second @01:35:31.94 AS.reconfigure ASfast @01:35:31.99 AS.seek 0ml @01:35:59.34 delay 3 seconds @01:36:02.37 AFV.select :carrierSolution @01:36:02.47 delay 2 seconds @01:36:04.50 AS.reconfigure ASconfig @01:36:04.55 AS.seek 1ml @01:37:02.34 delay 1 second @01:37:03.36 AFV.select :holdingCoil @01:37:03.46 delay 1 second @01:37:04.49 AS.reconfigure ASfast @01:37:04.54 AS.seek 0ml @01:37:31.90 delay 3 seconds @01:37:34.93 AFV.select :carrierSolution @01:37:35.03 delay 2 seconds @01:37:37.06 AS.reconfigure ASconfig @01:37:37.11 AS.seek 1ml @01:38:34.86 delay 1 second @01:38:35.88 AFV.select :holdingCoil @01:38:35.98 delay 1 second @01:38:37.01 AS.reconfigure ASnorm @01:38:37.06 AS.seek 0ml @01:39:27.78 Setting up PCR for IPc -- writing /var/log/esp/IPc20may1501h450ml.pcr @01:39:27.79 Building PCR Slug from ASV with MM3 mix, PP6 primer @01:39:27.81 Priming with carrier @01:39:27.82 ATV.dial ARV @01:39:28.40 ARV.dial :carrier @01:39:28.74 AS.seek 0.5ml @01:39:54.34 AFV.select :carrierSolution @01:39:54.45 delay 2 seconds @01:39:56.48 AS.reconfigure ASconfig @01:39:56.53 AS.seek 1ml @01:40:25.56 delay 1 second @01:40:26.58 AFV.select :holdingCoil @01:40:26.68 delay 1 second @01:40:27.71 ATV.dial :waste @01:40:29.28 AS.reconfigure ASnorm @01:40:29.33 AS.seek :empty @01:41:20.06 AFV.select :carrierSolution @01:41:20.17 delay 2 seconds @01:41:22.20 AS.reconfigure ASconfig @01:41:22.25 AS.seek 0.5ml @01:41:51.34 delay 1 second @01:41:52.36 AFV.select :holdingCoil @01:41:52.46 Building Slug @01:41:52.47 ATV.dial :ARV @01:41:54.02 delay 2 seconds @01:41:56.05 ARV.dial :air1 @01:41:57.03 delay 2 seconds @01:41:59.12 AS.reconfigure ASslow2 @01:41:59.18 AS.seek 0.506ml @01:42:05.33 delay 2 seconds @01:42:07.36 ARV.dial :MM3 @01:42:08.36 delay 2 seconds @01:42:10.39 Adding MM3 @01:42:10.47 AS.seek 0.524ml @01:42:28.86 delay 25 seconds @01:42:53.89 ARV.dial :PP6 @01:42:54.27 Adding PP6 @01:42:54.29 delay 2 seconds @01:42:56.37 AS.seek 0.53ml @01:43:02.80 delay 10 seconds @01:43:12.83 ARV.dial :air1 @01:43:13.63 adding ARV air @01:43:13.65 delay 2 seconds @01:43:15.73 AS.seek 0.536ml @01:43:22.13 delay 5 seconds @01:43:27.16 ARV.dial :carrier @01:43:27.55 delay 7 seconds @01:43:34.64 AS.seek 0.594ml @01:44:33.16 delay 5 seconds @01:44:38.19 ATV.dial :ASV @01:44:38.58 delay 2 seconds @01:44:40.67 AS.seek 0.6ml @01:44:46.89 delay 4 seconds @01:44:50.92 ATV.dial ARV @01:44:51.46 delay 2 seconds @01:44:53.49 ARV.dial :carrier @01:44:53.50 delay 2 seconds @01:44:55.59 AS.seek 0.65ml @01:45:46.07 delay 4 seconds @01:45:50.10 ATV.dial PCR @01:45:50.69 ASV.dial :waste1 @01:45:50.70 ARV.dial :air1 @01:45:51.09 Positioning Slug by 0.324ml @01:45:51.18 AS.seek 0.326ml @01:51:17.34 delay 5 seconds @01:51:22.37 ATV.dial ARV @01:51:23.21 ASV.dial :PCRlock @01:51:23.60 delay 5 seconds @01:51:28.63 Running IPc ProfileIPc thermocycling @01:51:28.64 PCR.program ProfileIPc @01:51:28.75 ProfileIPc should complete in < 1:36:15 @01:51:28.76 PCR.run 2:05:18.75 @03:09:38.08 PCR.off @03:09:38.63 PCR for IPc completed -- closed /var/log/esp/IPc20may1501h450ml.pcr @03:09:38.71 Cleaning PCR @03:09:38.72 AFV.select :carrierSolution @03:09:38.83 delay 2 seconds @03:09:40.86 AS.reconfigure ASconfig @03:09:40.91 AS.seek 0.5ml @03:09:51.24 delay 1 second @03:09:52.26 AFV.select :holdingCoil @03:09:52.36 ASV.dial :waste1 @03:09:52.74 ATV.dial :air3 @03:09:53.96 AS.reconfigure ASslow1 @03:09:54.01 AS.seek 0.51ml @03:09:56.28 delay 2 seconds @03:09:58.30 ATV.dial :bleach @03:09:58.90 AS.seek 0.81ml @03:10:59.47 delay 3 seconds @03:11:02.50 ATV.dial :air3 @03:11:03.04 delay 3 seconds @03:11:06.13 AS.seek 0.835ml @03:11:11.42 delay 3 seconds @03:11:14.45 ATV.dial :bleach @03:11:15.06 AS.seek 0.82ml @03:11:18.38 delay 3 seconds @03:11:21.40 ARV.dial :waste @03:11:21.99 delay 3 seconds @03:11:25.02 ATV.dial :ARV @03:11:25.98 AS.reconfigure ASconfig @03:11:26.03 AS.seek 0.47ml @03:11:46.43 AFV.select :carrierSolution @03:11:46.54 delay 2 seconds @03:11:48.58 AS.seek 1ml @03:12:19.36 delay 1 second @03:12:20.38 AFV.select :holdingCoil @03:12:20.48 delay 1 second @03:12:21.51 AS.reconfigure ASfast @03:12:21.56 AS.seek 0ml @03:12:48.91 AFV.select :carrierSolution @03:12:49.02 delay 2 seconds @03:12:51.05 AS.reconfigure ASconfig @03:12:51.10 AS.seek 1ml @03:13:48.88 delay 1 second @03:13:49.90 AFV.select :holdingCoil @03:13:50.00 delay 1 second @03:13:51.03 AS.reconfigure ASfast @03:13:51.08 AS.seek 0ml @03:14:18.43 AFV.select :carrierSolution @03:14:18.54 delay 2 seconds @03:14:20.57 AS.reconfigure ASconfig @03:14:20.62 AS.seek 1ml @03:15:18.41 delay 1 second @03:15:19.43 AFV.select :holdingCoil @03:15:19.53 delay 1 second @03:15:20.56 AS.reconfigure ASfast @03:15:20.61 AS.seek 0ml @03:15:47.95 ARV.dial between carrier and air1 @03:15:48.43 ATV.dial :air3 @03:15:49.66 AS.reconfigure ASslow1 @03:15:49.71 AS.seek 0.01ml @03:15:52.05 delay 2 seconds @03:15:54.07 ATV.dial :bleach @03:15:54.67 AS.seek 0.81ml @03:18:35.79 delay 3 seconds @03:18:38.81 ATV.dial :air3 @03:18:39.35 delay 3 seconds @03:18:42.44 AS.seek 0.835ml @03:18:47.73 delay 3 seconds @03:18:50.75 ATV.dial :bleach @03:18:51.36 AS.seek 0.82ml @03:18:54.67 delay 3 seconds @03:18:57.70 ATV.dial PCR @03:18:58.09 AS.reconfigure ASconfig @03:18:58.14 AS.seek :empty @03:19:45.57 PCR.program :bleachProfile @03:19:45.63 PCR program should complete in < 2:45 @03:19:45.64 PCR.run 10:00 @03:23:00.16 PCR.off @03:23:00.18 AFV.select :carrierSolution @03:23:00.29 delay 2 seconds @03:23:02.33 AS.seek 1ml @03:24:00.11 delay 1 second @03:24:01.13 AFV.select :holdingCoil @03:24:01.23 delay 1 second @03:24:02.26 AS.reconfigure ASfast @03:24:02.31 AS.seek 0ml @03:24:29.64 delay 3 seconds @03:24:32.67 AFV.select :carrierSolution @03:24:32.77 delay 2 seconds @03:24:34.80 AS.reconfigure ASconfig @03:24:34.86 AS.seek 1ml @03:25:32.64 delay 1 second @03:25:33.66 AFV.select :holdingCoil @03:25:33.76 delay 1 second @03:25:34.79 AS.reconfigure ASfast @03:25:34.84 AS.seek 0ml @03:26:02.20 delay 3 seconds @03:26:05.23 AFV.select :carrierSolution @03:26:05.33 delay 2 seconds @03:26:07.36 AS.reconfigure ASconfig @03:26:07.41 AS.seek 1ml @03:27:05.16 delay 1 second @03:27:06.18 AFV.select :holdingCoil @03:27:06.28 delay 1 second @03:27:07.31 AS.reconfigure ASfast @03:27:07.36 AS.seek 0ml @03:27:34.72 delay 3 seconds @03:27:37.75 AFV.select :carrierSolution @03:27:37.85 delay 2 seconds @03:27:39.88 AS.reconfigure ASconfig @03:27:39.94 AS.seek 1ml @03:28:37.77 delay 1 second @03:28:38.79 AFV.select :holdingCoil @03:28:38.89 delay 1 second @03:28:39.92 AS.reconfigure ASfast @03:28:39.97 AS.seek 0ml @03:29:07.30 delay 3 seconds @03:29:10.33 AFV.select :carrierSolution @03:29:10.43 delay 2 seconds @03:29:12.46 AS.reconfigure ASconfig @03:29:12.51 AS.seek 1ml @03:30:10.30 delay 1 second @03:30:11.32 AFV.select :holdingCoil @03:30:11.42 delay 1 second @03:30:12.45 AS.reconfigure ASnorm @03:30:12.50 AS.seek 0ml @03:31:03.24 Setting up PCR for IPc2 -- writing /var/log/esp/IPc220may1503h450ml.pcr @03:31:03.26 Building PCR Slug from ASV with MM2 mix, PP3 primer @03:31:03.27 Priming with carrier @03:31:03.28 ATV.dial ARV @03:31:03.86 ARV.dial :carrier @03:31:04.20 AS.seek 0.5ml @03:31:29.77 AFV.select :carrierSolution @03:31:29.88 delay 2 seconds @03:31:31.91 AS.reconfigure ASconfig @03:31:31.96 AS.seek 1ml @03:32:00.97 delay 1 second @03:32:01.99 AFV.select :holdingCoil @03:32:02.09 delay 1 second @03:32:03.12 ATV.dial :waste @03:32:04.69 AS.reconfigure ASnorm @03:32:04.74 AS.seek :empty @03:32:55.50 AFV.select :carrierSolution @03:32:55.61 delay 2 seconds @03:32:57.64 AS.reconfigure ASconfig @03:32:57.69 AS.seek 0.5ml @03:33:26.79 delay 1 second @03:33:27.81 AFV.select :holdingCoil @03:33:27.91 Building Slug @03:33:27.92 ATV.dial :ARV @03:33:29.46 delay 2 seconds @03:33:31.49 ARV.dial :air1 @03:33:31.88 delay 2 seconds @03:33:33.96 AS.reconfigure ASslow2 @03:33:34.01 AS.seek 0.506ml @03:33:40.18 delay 2 seconds @03:33:42.20 ARV.dial :MM2 @03:33:43.60 delay 2 seconds @03:33:45.63 Adding MM2 @03:33:45.70 AS.seek 0.524ml @03:34:04.01 delay 25 seconds @03:34:29.04 ARV.dial :PP3 @03:34:29.43 Adding PP3 @03:34:29.44 delay 2 seconds @03:34:31.53 AS.seek 0.53ml @03:34:37.79 delay 10 seconds @03:34:47.82 ARV.dial :air2 @03:34:48.21 adding ARV air @03:34:48.22 delay 2 seconds @03:34:50.31 AS.seek 0.536ml @03:34:56.52 delay 5 seconds @03:35:01.55 ARV.dial :carrier @03:35:02.76 delay 7 seconds @03:35:09.85 AS.seek 0.594ml @03:36:08.37 delay 5 seconds @03:36:13.41 ATV.dial :ASV @03:36:13.79 delay 2 seconds @03:36:15.88 AS.seek 0.6ml @03:36:22.30 delay 4 seconds @03:36:26.33 ATV.dial ARV @03:36:27.43 delay 2 seconds @03:36:29.46 ARV.dial :carrier @03:36:29.47 delay 2 seconds @03:36:31.56 AS.seek 0.65ml @03:37:22.10 delay 4 seconds @03:37:26.13 ATV.dial PCR @03:37:26.71 ASV.dial :waste1 @03:37:26.72 ARV.dial :air1 @03:37:27.13 Positioning Slug by 0.324ml @03:37:27.22 AS.seek 0.326ml @03:42:53.50 delay 5 seconds @03:42:58.53 ATV.dial ARV @03:42:59.26 ASV.dial :PCRlock @03:42:59.65 delay 5 seconds @03:43:04.68 Running IPc2 ProfileIPc thermocycling @03:43:04.69 PCR.program ProfileIPc @03:43:04.79 ProfileIPc should complete in < 1:36:15 @03:43:04.80 PCR.run 2:05:18.75 @05:01:12.17 PCR.off @05:01:12.80 PCR for IPc2 completed -- closed /var/log/esp/IPc220may1503h450ml.pcr @05:01:12.82 Full MFB Decontamination @05:01:12.83 Decon extraction column and decon AIV @05:01:12.85 SPE.hold 2:46:40,55C @05:01:12.90 ASV.dial :SPEdelivery @05:01:13.51 delay 20 seconds @05:01:33.54 AFV.select :carrierSolution @05:01:33.64 delay 2 seconds @05:01:35.67 AS.reconfigure ASconfig @05:01:35.72 AS.seek 0.5ml @05:01:46.01 delay 1 second @05:01:47.03 AFV.select :holdingCoil @05:01:47.13 ATV.dial :air1 @05:01:48.81 AS.reconfigure ASslow1 @05:01:48.86 AS.seek 0.52ml @05:01:53.13 ATV.dial :ASV @05:01:54.68 ASV.dial :SPErecovery @05:01:55.92 AS.seek 0.62ml @05:02:16.29 ATV.dial :waste @05:02:18.05 AS.reconfigure ASnorm @05:02:18.10 AS.seek :empty @05:02:49.71 AFV.select :holdingCoil @05:02:49.72 ATV.dial :waste @05:02:49.74 AS.reconfigure ASconfig @05:02:49.79 AS.seek 0ml @05:02:50.12 AFV.select :carrierSolution @05:02:50.23 delay 2 seconds @05:02:52.27 AS.seek 0.5ml @05:03:21.29 delay 1 second @05:03:22.31 AFV.select :holdingCoil @05:03:22.41 ATV.dial :air3 @05:03:23.17 AS.reconfigure ASslow1 @05:03:23.22 AS.seek 0.52ml @05:03:27.49 ATV.dial :air3 @05:03:27.58 AS.seek 0.53ml @05:03:29.84 delay 2 seconds @05:03:31.86 ATV.dial :bleach @05:03:32.33 AS.seek 0.71ml @05:04:08.79 delay 3 seconds @05:04:11.82 ATV.dial :air3 @05:04:12.35 delay 3 seconds @05:04:15.44 AS.seek 0.735ml @05:04:20.76 delay 3 seconds @05:04:23.79 ATV.dial :bleach @05:04:24.38 AS.seek 0.72ml @05:04:27.68 delay 3 seconds @05:04:30.71 ATV.dial ASV @05:04:31.70 ASV.dial :SPErecovery @05:04:31.79 AS.seek 0.365ml @05:05:43.39 ATV.dial :waste @05:05:44.99 AS.reconfigure ASnorm @05:05:45.04 AS.seek :empty @05:06:03.81 ATV.dial :air3 @05:06:04.47 AS.reconfigure ASslow1 @05:06:04.52 AS.seek 0.009ml @05:06:06.79 delay 2 seconds @05:06:08.81 ATV.dial :bleach @05:06:09.27 AS.seek 0.579ml @05:08:04.17 delay 3 seconds @05:08:07.20 ATV.dial :air3 @05:08:07.73 delay 3 seconds @05:08:10.82 AS.seek 0.604ml @05:08:16.13 delay 3 seconds @05:08:19.16 ATV.dial :bleach @05:08:19.75 AS.seek 0.589ml @05:08:23.03 delay 3 seconds @05:08:26.06 ATV.dial :ASV @05:08:27.04 ASV.dial :wasteR @05:08:27.43 AS.seek 0ml @05:10:26.28 delay 1:00 @05:11:26.31 ATV.dial ASV @05:11:26.32 AFV.select :carrierSolution @05:11:26.42 delay 2 seconds @05:11:28.45 AS.reconfigure ASconfig @05:11:28.50 AS.seek 1ml @05:12:26.28 delay 1 second @05:12:27.30 AFV.select :holdingCoil @05:12:27.40 delay 1 second @05:12:28.43 ASV.dial :wasteR @05:12:28.45 AS.reconfigure ASslow1 @05:12:28.49 AS.seek 0ml @05:15:49.99 delay 10 seconds @05:16:00.02 ATV.dial :air3 @05:16:01.46 AS.seek 0.01ml @05:16:03.74 delay 2 seconds @05:16:05.76 ATV.dial :bleach @05:16:06.37 AS.seek 0.58ml @05:18:01.25 delay 3 seconds @05:18:04.28 ATV.dial :air3 @05:18:04.82 delay 3 seconds @05:18:07.91 AS.seek 0.605ml @05:18:13.23 delay 3 seconds @05:18:16.26 ATV.dial :bleach @05:18:16.87 AS.seek 0.59ml @05:18:20.16 delay 3 seconds @05:18:23.19 ATV.dial ASV @05:18:24.18 ASV.dial :SPEdelivery @05:18:24.56 AS.seek 0ml @05:20:23.50 delay 1:00 @05:21:23.53 AFV.select :carrierSolution @05:21:23.63 delay 2 seconds @05:21:25.66 AS.reconfigure ASconfig @05:21:26.29 AS.seek 1ml @05:22:24.07 delay 1 second @05:22:25.09 AFV.select :holdingCoil @05:22:25.19 delay 1 second @05:22:26.22 ATV.dial ASV @05:22:26.23 ASV.dial :SPEdelivery @05:22:26.25 AS.reconfigure ASslow1 @05:22:26.30 AS.seek 0ml @05:25:47.68 delay 10 seconds @05:25:57.71 ATV.dial :air2 @05:25:58.50 AS.reconfigure ASslow0 @05:25:58.55 AS.seek 0.8ml @05:27:19.34 delay 3 seconds @05:27:22.37 ATV.dial :ASV @05:27:23.31 ASV.dial :SPEdelivery @05:27:23.33 AS.reconfigure ASslow1 @05:27:23.38 AS.seek 0.03ml @05:29:58.53 delay 20 seconds @05:30:18.56 ATV.dial :waste,via: :air3 @05:30:20.18 AS.reconfigure ASconfig @05:30:20.23 AS.seek :empty @05:30:22.29 SPE.coast @05:30:22.38 Removing Bleach from Mixing Coil @05:30:22.39 AFV.select :carrierSolution @05:30:22.49 delay 2 seconds @05:30:24.53 AS.seek 0.5ml @05:30:53.64 delay 1 second @05:30:54.66 AFV.select :holdingCoil @05:30:54.76 ATV.dial :ASV @05:30:56.36 ASV.dial :SPErecovery @05:30:57.00 AS.reconfigure ASslow1 @05:30:57.05 AS.seek 0.73ml @05:31:43.56 ASV.dial :wasteD @05:31:44.12 AS.reconfigure ASnorm @05:31:44.17 AS.seek 0ml @05:32:21.29 AFV.select :carrierSolution @05:32:21.40 delay 2 seconds @05:32:23.43 AS.reconfigure ASconfig @05:32:23.48 AS.seek 0.5ml @05:32:52.51 delay 1 second @05:32:53.53 AFV.select :holdingCoil @05:32:53.63 ATV.dial ASV @05:32:53.64 ASV.dial :SPErecovery @05:32:54.29 AS.reconfigure ASslow0 @05:32:54.34 AS.seek 0.3ml @05:33:14.81 ATV.dial :air2 @05:33:15.86 AS.reconfigure ASslow1 @05:33:15.91 AS.seek 0.32ml @05:33:20.24 ATV.dial ASV @05:33:21.24 AS.reconfigure ASslow0 @05:33:21.29 AS.seek 0.57ml @05:33:46.76 ASV.dial :wasteD @05:33:47.33 AS.reconfigure ASnorm @05:33:47.38 AS.seek 0ml @05:34:16.44 AFV.select :carrierSolution @05:34:16.55 delay 2 seconds @05:34:18.58 AS.reconfigure ASconfig @05:34:18.63 AS.seek 0.5ml @05:34:47.67 delay 1 second @05:34:48.69 AFV.select :holdingCoil @05:34:48.79 ATV.dial ASV @05:34:48.80 ASV.dial :SPErecovery @05:34:49.44 AS.reconfigure ASslow0 @05:34:49.49 AS.seek 0.3ml @05:35:09.92 ATV.dial :air2 @05:35:10.91 AS.reconfigure ASslow1 @05:35:10.96 AS.seek 0.32ml @05:35:15.31 ATV.dial ASV @05:35:16.30 AS.reconfigure ASslow0 @05:35:16.35 AS.seek 0.57ml @05:35:41.81 ASV.dial :wasteD @05:35:42.45 AS.reconfigure ASnorm @05:35:42.50 AS.seek 0ml @05:36:11.58 ATV.dial :air2 @05:36:12.51 AS.reconfigure ASslow0 @05:36:12.56 AS.seek 0.3ml @05:36:43.09 ATV.dial :ASV @05:36:44.01 ASV.dial :wasteD @05:36:44.03 AS.reconfigure ASconfig @05:36:44.08 AS.seek 0.05ml @05:36:58.76 ATV.dial :waste @05:37:00.53 AS.seek 0ml @05:37:03.69 ASV.dial :SPEdelivery @05:37:04.66 deconLysateINT @05:37:04.67 ATV.dial :air3 @05:37:05.35 AS.reconfigure ASslow1 @05:37:05.40 AS.seek 0.01ml @05:37:07.69 delay 2 seconds @05:37:09.71 ATV.dial :bleach @05:37:10.74 AS.seek 0.96ml @05:40:22.05 delay 3 seconds @05:40:25.08 ATV.dial :air3 @05:40:25.61 delay 3 seconds @05:40:28.71 AS.seek 0.985ml @05:40:34.00 delay 3 seconds @05:40:37.03 ATV.dial :bleach @05:40:37.63 AS.seek 0.97ml @05:40:40.92 delay 3 seconds @05:40:43.95 ATV.dial AIV @05:40:45.24 AIV.dial :coilToMFB @05:40:45.26 AWV.select :waste @05:40:45.27 AFV.select :carrierSolution @05:40:45.38 delay 2 seconds @05:40:47.41 AS.reconfigure ASconfig @05:40:47.46 AS.seek 1ml @05:40:49.53 delay 1 second @05:40:50.55 AFV.select :holdingCoil @05:40:50.65 delay 1 second @05:40:51.68 AS.reconfigure ASslow0 @05:40:51.73 AS.seek :empty @05:42:32.67 AFV.select :carrierSolution @05:42:32.78 delay 2 seconds @05:42:34.81 AS.reconfigure ASconfig @05:42:34.86 AS.seek 1ml @05:43:32.69 delay 1 second @05:43:33.71 AFV.select :holdingCoil @05:43:33.81 delay 1 second @05:43:34.84 AS.reconfigure ASslow0 @05:43:34.89 AS.seek :empty @05:45:15.85 AFV.select :carrierSolution @05:45:15.96 delay 2 seconds @05:45:17.99 AS.reconfigure ASconfig @05:45:18.04 AS.seek 1ml @05:46:15.85 delay 1 second @05:46:16.87 AFV.select :holdingCoil @05:46:16.97 delay 1 second @05:46:18.00 AS.reconfigure ASslow0 @05:46:18.05 AS.seek :empty @05:47:59.04 ATV.dial :air2 @05:47:59.70 AS.reconfigure ASslow1 @05:47:59.75 AS.seek 0.009ml @05:48:02.16 AS.reconfigure ASslow0 @05:48:02.21 AS.seek 1ml @05:49:42.18 ATV.dial AIV @05:49:42.94 AS.seek 0.02ml @05:51:21.84 ATV.dial :waste @05:51:23.41 AS.seek 0ml @05:51:25.70 AWV.select :air @05:51:25.87 Cleaning PCR @05:51:25.88 AFV.select :carrierSolution @05:51:25.99 delay 2 seconds @05:51:28.02 AS.reconfigure ASconfig @05:51:28.07 AS.seek 0.5ml @05:51:57.10 delay 1 second @05:51:58.12 AFV.select :holdingCoil @05:51:58.22 ASV.dial :waste1 @05:51:58.59 ATV.dial :air3 @05:51:59.25 AS.reconfigure ASslow1 @05:51:59.30 AS.seek 0.51ml @05:52:01.57 delay 2 seconds @05:52:03.59 ATV.dial :bleach @05:52:04.06 AS.seek 0.81ml @05:53:04.74 delay 3 seconds @05:53:07.77 ATV.dial :air3 @05:53:08.31 delay 3 seconds @05:53:11.40 AS.seek 0.835ml @05:53:16.70 delay 3 seconds @05:53:19.73 ATV.dial :bleach @05:53:20.33 AS.seek 0.82ml @05:53:23.63 delay 3 seconds @05:53:26.65 ARV.dial :waste @05:53:27.23 delay 3 seconds @05:53:30.26 ATV.dial :ARV @05:53:31.11 AS.reconfigure ASconfig @05:53:31.16 AS.seek 0.47ml @05:53:51.56 AFV.select :carrierSolution @05:53:51.67 delay 2 seconds @05:53:53.71 AS.seek 1ml @05:54:24.54 delay 1 second @05:54:25.56 AFV.select :holdingCoil @05:54:25.66 delay 1 second @05:54:26.69 AS.reconfigure ASfast @05:54:26.74 AS.seek 0ml @05:54:54.06 AFV.select :carrierSolution @05:54:54.17 delay 2 seconds @05:54:56.20 AS.reconfigure ASconfig @05:54:56.25 AS.seek 1ml @05:55:54.00 delay 1 second @05:55:55.02 AFV.select :holdingCoil @05:55:55.12 delay 1 second @05:55:56.15 AS.reconfigure ASfast @05:55:56.20 AS.seek 0ml @05:56:23.53 AFV.select :carrierSolution @05:56:23.64 delay 2 seconds @05:56:25.67 AS.reconfigure ASconfig @05:56:25.72 AS.seek 1ml @05:57:23.53 delay 1 second @05:57:24.55 AFV.select :holdingCoil @05:57:24.65 delay 1 second @05:57:25.68 AS.reconfigure ASfast @05:57:26.31 AS.seek 0ml @05:57:53.67 ARV.dial between carrier and air1 @05:57:54.15 ATV.dial :air3 @05:57:55.40 AS.reconfigure ASslow1 @05:57:55.45 AS.seek 0.01ml @05:57:57.73 delay 2 seconds @05:57:59.75 ATV.dial :bleach @05:58:00.36 AS.seek 0.81ml @06:00:41.55 delay 3 seconds @06:00:44.58 ATV.dial :air3 @06:00:45.12 delay 3 seconds @06:00:48.21 AS.seek 0.835ml @06:00:53.51 delay 3 seconds @06:00:56.54 ATV.dial :bleach @06:00:57.15 AS.seek 0.82ml @06:01:00.50 delay 3 seconds @06:01:03.53 ATV.dial PCR @06:01:03.92 AS.reconfigure ASconfig @06:01:03.97 AS.seek :empty @06:01:51.44 PCR.program :bleachProfile @06:01:51.49 PCR program should complete in < 2:45 @06:01:51.50 PCR.run 10:00 @06:05:20.59 PCR.off @06:05:20.62 AFV.select :carrierSolution @06:05:20.73 delay 2 seconds @06:05:22.77 AS.seek 1ml @06:06:20.64 delay 1 second @06:06:21.67 AFV.select :holdingCoil @06:06:21.77 delay 1 second @06:06:22.80 AS.reconfigure ASfast @06:06:22.85 AS.seek 0ml @06:06:50.19 delay 3 seconds @06:06:53.22 AFV.select :carrierSolution @06:06:53.32 delay 2 seconds @06:06:55.35 AS.reconfigure ASconfig @06:06:55.44 AS.seek 1ml @06:07:53.24 delay 1 second @06:07:54.26 AFV.select :holdingCoil @06:07:54.36 delay 1 second @06:07:55.39 AS.reconfigure ASfast @06:07:55.44 AS.seek 0ml @06:08:22.77 delay 3 seconds @06:08:25.80 AFV.select :carrierSolution @06:08:25.90 delay 2 seconds @06:08:27.93 AS.reconfigure ASconfig @06:08:27.99 AS.seek 1ml @06:09:25.80 delay 1 second @06:09:26.82 AFV.select :holdingCoil @06:09:26.92 delay 1 second @06:09:27.95 AS.reconfigure ASfast @06:09:28.00 AS.seek 0ml @06:09:55.33 delay 3 seconds @06:09:58.36 AFV.select :carrierSolution @06:09:58.46 delay 2 seconds @06:10:00.49 AS.reconfigure ASconfig @06:10:00.54 AS.seek 1ml @06:10:58.35 delay 1 second @06:10:59.37 AFV.select :holdingCoil @06:10:59.47 delay 1 second @06:11:00.50 AS.reconfigure ASfast @06:11:00.55 AS.seek 0ml @06:11:27.88 delay 3 seconds @06:11:30.91 AFV.select :carrierSolution @06:11:31.01 delay 2 seconds @06:11:33.04 AS.reconfigure ASconfig @06:11:33.10 AS.seek 1ml @06:12:30.85 delay 1 second @06:12:31.87 AFV.select :holdingCoil @06:12:31.97 delay 1 second @06:12:33.00 AS.reconfigure ASnorm @06:12:33.05 AS.seek 0ml @06:13:23.75 AFV.select :carrierSolution @06:13:23.86 delay 2 seconds @06:13:25.89 AS.reconfigure ASconfig @06:13:25.94 AS.seek 1ml @06:14:23.73 delay 1 second @06:14:24.75 AFV.select :holdingCoil @06:14:24.85 delay 1 second @06:14:25.88 ATV.dial :waste @06:14:27.04 AS.reconfigure ASnorm @06:14:27.09 AS.seek 0.5ml @06:14:52.66 ATV.dial :waste2 @06:14:53.88 AS.seek 0ml @06:15:19.40 AS.coast @06:15:19.45 SS.coast @06:15:19.48 CC.coast @06:15:19.52 CS.coast @06:15:19.56 PC.coast @06:15:19.60 PS.coast @06:15:19.64 Forearm.coast @06:15:19.68 Elbow.coast @06:15:20.08 Gate.power :core,:OFF @06:15:20.10 Gate.power :raw,:OFF @06:15:20.11 Shutdown @06:15:20.12 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @09:58:50.94 Session Begun @10:00:00.10
Powering core bus @10:00:04.91 Gate.power :raw,:ON @10:00:05.60 SPE.coast @10:00:05.69 SPE.configure SPEconfig @10:00:05.80 AS.configure ASconfig @10:00:05.93 SS.configure SSconfig @10:00:06.04 CC.configure CCconfig @10:00:06.17 CS.configure CSconfig @10:00:06.29 PC.configure PCconfig @10:00:06.41 PS.configure PSconfig @10:00:06.55 Forearm.configure LooseForearm @10:00:06.67 Elbow.configure LooseElbow @10:00:06.81 SC.configure SCloose @10:00:06.93 SE.configure SEconfig @10:00:07.00 CH.coast @10:00:07.07 CH.configure CHconfig @10:00:07.13 PH.coast @10:00:07.21 PH.configure PHconfig @10:00:07.27 PCR.off @10:00:07.32 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:07.37 PCR.caltemp= 2.8814,0.0372,0 @10:00:07.41 All dwarf controllers running firmware version 3.75 @10:00:07.55 Valve.reset @10:00:07.75 PCR.off @10:00:07.80 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:07.84 PCR.caltemp= 2.8814,0.0372,0 @10:00:08.06 Intake.select :close @10:00:08.10 Exhaust.select :close @10:00:08.12 CTV.dial :air @10:00:08.25 PSV.dial :air @10:00:08.28 CSV.dial :waste1 @10:00:08.46
Forearm.reconfigure LooseForearm @10:00:08.56 SSV.dial :spit,avoiding: :exhaust @10:00:08.58
Forearm.home.seek :retract @10:00:08.84 PS.reconfigure PSconfig @10:00:08.91 PS.home.seek 0ml @10:00:09.41 Hand.close @10:00:09.46
Elbow.jog 1500 @10:00:09.64 CS.reconfigure CSfast @10:00:09.68 SS.reconfigure SSconfig @10:00:09.78 CS.home.seek 0ml @10:00:09.85 SS.home.seek 0ml @10:00:10.22
Elbow.reconfigure LooseElbow @10:00:10.29 Elbow.home.seek :clear @10:00:13.75 SE.reconfigure SEconfig @10:00:13.82 SE.home.seek :down @10:00:14.53 SC.reconfigure SCsloppy @10:00:14.60 SC.home.seek :initial @10:00:16.82 Forearm.seek :retract @10:00:16.92 Elbow.seek :hammer @10:00:17.94 PS.coast @10:00:18.25
Forearm.reconfigure TightForearm @10:00:18.33 Forearm.seek 5 + 350 counts @10:00:19.48 Forearm.seek :retract @10:00:20.81 CC.reconfigure CCconfig @10:00:20.82 PC.reconfigure PCconfig @10:00:20.94
CC.home.seek :open @10:00:20.98 PC.home.seek :open @10:00:24.34
Phase [stdphase] @10:00:24.36 GoodNews.email "At 10:00:24 began in tube 2: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:119\n{1 => 6, 2 => 16}\nCan@10:00:24, 8.4C, 41% humidity, 14.3psia, 14.547V, 0.764A, 0.764A avg, 11.12W",Subject: "Started 20invasivedeployfinal phase stdphase" @10:00:24.41CEST15-May-20
===> STARTED Superphase (phase #2) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @10:00:24.46 Starting initialPurge @10:00:24.49 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:00:24.59 Hand.open @10:00:25.38 Elbow.reconfigure TightElbow @10:00:25.53 Elbow.seek FlushPuck::Garage @10:00:27.58 Elbow.reconfigure LooseElbow @10:00:27.72 Forearm.reconfigure LooseForearm @10:00:27.79 Forearm.seek :garage @10:00:28.82 Hand.close @10:00:29.66 Forearm.seek :retract @10:00:30.74 Elbow.reconfigure TightElbow @10:00:30.92 Elbow.seek CC @10:00:34.23 Elbow.reconfigure LooseElbow @10:00:34.53 Forearm.reconfigure TightForearm @10:00:34.66 Forearm.seek CC @10:00:36.42 CC.seek :closed @10:00:40.81 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 1.83 @10:00:41.07 Hand.open @10:00:41.91 Forearm.reconfigure LooseForearm @10:00:42.05 Forearm.seek :retract @10:00:43.19 Flushing the Collection Valve::Manifold @10:00:43.50 CSV.dial CSR @10:00:45.01 Pulling 4.999ml of CSR.flush @10:00:45.04 CSR.select :flush @10:00:45.31 CS.reconfigure CSconfig @10:00:45.44 CS.seek 5ml @10:00:59.28 delay 5 seconds @10:01:04.31 CSR.select :air @10:01:04.60 CS.reconfigure CSfast @10:01:04.74 CS.seek 7.5ml @10:01:11.19 CSV.dial :waste @10:01:12.56 CS.reconfigure CSconfig @10:01:12.68 CS.seek 0ml @10:01:33.02 Purging external Intake @10:01:33.17 CSV.dial :flush @10:01:33.66 CS.reconfigure CSfast @10:01:33.73 CS.seek 10ml @10:01:55.80 delay 5 seconds @10:02:00.83 CSV.dial CBV @10:02:00.87
CBV.dial CSV @10:02:01.66 CTV.dial :intake @10:02:02.44 Intake.select :open @10:02:02.58 CS.seek 0ml @10:02:26.43 Intake.select :close @10:02:26.56 CTV.dial :air @10:02:27.33 Flushing upper manifold @10:02:27.49 CSV.dial :flush @10:02:28.25 CS.seek 5ml @10:02:39.90 delay 5 seconds @10:02:44.93 CSV.dial :air @10:02:46.11 CS.seek 7ml @10:02:51.49 CSV.dial CBV @10:02:51.52
CBV.dial CSV @10:02:52.55 CTV.dial :air @10:02:52.58 CS.seek 0ml @10:03:08.46 delay 7 seconds @10:03:15.50 CS.seek 3ml @10:03:22.90 CSV.dial :waste @10:03:23.39 CS.seek 0ml @10:03:30.99 CSV.dial :flush @10:03:31.47 CS.seek 3ml @10:03:38.90 delay 5 seconds @10:03:43.93 CSV.dial :air @10:03:44.48 CS.seek 5ml @10:03:49.90 CSV.dial :air @10:03:49.93 CS.seek 0ml @10:04:01.62 delay 7 seconds @10:04:08.65 CS.seek 3ml @10:04:16.05 CSV.dial :waste @10:04:16.81 CS.seek 0ml @10:04:24.30 Garaging FlushPuck in Collection Clamp @10:04:24.41 Elbow.reconfigure TightElbow @10:04:24.47 Elbow.seek CC @10:04:24.74 Elbow.reconfigure LooseElbow @10:04:24.87 Forearm.seek CC @10:04:25.89 Hand.close @10:04:26.51 CC.seek :open @10:04:30.90 Forearm.seek :retract @10:04:31.89 Elbow.reconfigure TightElbow @10:04:31.97 Elbow.seek :garage @10:04:35.20 Elbow.reconfigure LooseElbow @10:04:35.32 Forearm.reconfigure TightForearm @10:04:35.40 Forearm.seek :garage @10:04:36.57 Hand.open @10:04:37.21 Forearm.reconfigure LooseForearm @10:04:37.28 Forearm.seek :retract @10:04:38.41 Starting mfb @10:04:38.43 pcrBAC sampling at most 1500ml, wcr at most 1500ml @10:04:38.50 BACfans.email "Started mfb in tube 2 at 10:04:38CEST15-May-20\n{1 => 6, 2 => 16}\nCan@10:04:07, 8.4C, 41% humidity, 14.3psia, 14.562V, 0.670A, 0.670A avg, 9.75W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @10:04:38.55
Loading sh1 puck from tube 2 into Collection Clamp @10:04:38.75 Readying the MFB @10:04:38.82 Homing MFB Syringe @10:04:38.83 AFV.select :holdingCoil @10:04:39.14 ATV.dial :air2 @10:04:39.29
SC.reconfigure SCloose @10:04:39.59 Elbow.reconfigure TightElbow @10:04:39.74 Elbow.seek 2 @10:04:39.95 AS.reconfigure ASconfig @10:04:40.01 AS.home.seek :home @10:04:41.99
Forearm.reconfigure TightForearm @10:04:42.07 Forearm.seek 2 + 700 counts @10:04:43.16 SE.reconfigure SEpick @10:04:44.10 SE.seek beamBreak @10:04:45.57 AFV.select :carrierSolution @10:04:45.88 delay 2 seconds @10:04:45.97
SE.configure SEconfig @10:04:46.44 Elbow.seek 2 @10:04:46.91 Forearm.seek 2 + 300 counts @10:04:47.27 Forearm.reconfigure LooseForearm @10:04:47.43 Hand.close @10:04:47.94 AS.seek 1ml @10:04:48.20 SE.seek :down @10:04:48.78
Forearm.seek :retract @10:04:49.82 Elbow.seek CC @10:04:50.07 SE.coast @10:04:51.74
Elbow.reconfigure LooseElbow @10:04:52.06 Forearm.reconfigure TightForearm @10:04:52.21 Forearm.seek CC @10:04:54.09 CC.seek :closed @10:04:58.55 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.92 @10:04:58.58 Hand.open @10:04:59.45 Forearm.reconfigure LooseForearm @10:04:59.58 Forearm.seek :retract @10:05:00.67 SC.coast @10:05:00.83 collectSample 1500ml w/bubble point of 55psi @10:05:00.85 Priming 7ml @10:05:00.91 Intake.select :close @10:05:00.95 Exhaust.select :close @10:05:01.03 SS.hold @10:05:01.63 Calibrating pressure and temperature sensors @10:05:01.67 CTV.dial :air @10:05:01.74 SSV.dial :spit,avoiding: :exhaust @10:05:01.82 SS.reconfigure SSfast @10:05:02.01 SS.seek 5ml @10:05:04.87 SSV.dial CBV @10:05:04.96
CBV.dial SSV @10:05:06.48 delay 3.8 seconds @10:05:10.56 SSV.dial :spit @10:05:12.07 SS.seek 0ml @10:05:14.75 SSV.dial CBV @10:05:16.19 CTV.dial :intake @10:05:17.10 SSV.dial CBV @10:05:17.16
CBV.dial SSV @10:05:17.22 Allowing 40s for 7.0ml of remaining 7.0ml @10:05:17.27 Intake.select :open @10:05:19.58 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:05:19.63 SS.reconfigure SSinhale.with maxSpeed: 150 @10:05:19.83 SS.seek 7ml @10:05:23.37 Vacuum too high -- waiting up to 40s @10:05:27.42 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:05:27.46 SS.reconfigure SSinhale.with maxSpeed: 135 @10:05:27.67 SS.seek 7ml @10:05:31.23 Stroke completed -- waiting for pressure to equalize ... @10:05:36.71 Intake.select :close @10:05:37.00 SSV.dial :exhaust @10:05:37.87 Exhaust.select :open @10:05:38.09 SS.reconfigure SSexhale @10:05:38.23 SS.seek 0ml @10:05:40.93 delay 1 second @10:05:41.96 AFV.select :holdingCoil @10:05:42.06
Exhaust.select :close @10:05:42.27 delay 1 second @10:05:42.34
Sampling 1500.0ml @10:05:42.36 Intake.select :close @10:05:42.40 Exhaust.select :close @10:05:42.48 SS.reconfigure SSconfig @10:05:42.63 SS.hold @10:05:43.09 CTV.dial :intake @10:05:43.14 SSV.dial CBV @10:05:43.22
CBV.dial SSV @10:05:43.37 ATV.dial :waste @10:05:44.10
Allowing 130s for 25.0ml of remaining 1500.0ml @10:05:44.13 Intake.select :open @10:05:44.46 AS.reconfigure ASnorm @10:05:44.61 AS.home.seek :empty @10:05:46.47
Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:05:46.51 SS.reconfigure SSinhale.with maxSpeed: 135 @10:05:46.68 SS.seek 25ml @10:05:50.02 Vacuum too high -- waiting up to 40s @10:05:52.34 Intake @30.7psia, vacuum from 20.0 to 24.0psi @10:05:52.37 SS.reconfigure SSinhale.with maxSpeed: 122 @10:05:52.46 SS.seek 25ml @10:05:55.36 Vacuum too high -- waiting up to 40s @10:05:57.94 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:05:57.96 SS.reconfigure SSinhale.with maxSpeed: 110 @10:05:58.06 SS.seek 25ml @10:06:00.83 Vacuum too high -- waiting up to 40s @10:06:03.15 Intake @30.8psia, vacuum from 20.0 to 24.0psi @10:06:03.77 SS.reconfigure SSinhale.with maxSpeed: 99 @10:06:03.87 SS.seek 25ml @10:06:07.16 Vacuum too high -- waiting up to 40s @10:06:09.55 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:06:09.58 SS.reconfigure SSinhale.with maxSpeed: 89 @10:06:09.67 SS.seek 25ml @10:06:12.42 Vacuum too high -- waiting up to 40s @10:06:14.51 Intake @30.8psia, vacuum from 20.0 to 24.0psi @10:06:14.53 SS.reconfigure SSinhale.with maxSpeed: 80 @10:06:14.62 SS.seek 25ml @10:06:17.98 Vacuum too high -- waiting up to 40s @10:06:20.31 Intake @30.8psia, vacuum from 20.0 to 24.0psi @10:06:20.33 SS.reconfigure SSinhale.with maxSpeed: 72 @10:06:20.42 SS.seek 25ml @10:06:24.22 Vacuum too high -- waiting up to 40s @10:06:26.55 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:06:26.57 SS.reconfigure SSinhale.with maxSpeed: 65 @10:06:26.67 SS.seek 25ml @10:06:29.95 Vacuum too high -- waiting up to 40s @10:06:32.01 Intake @30.8psia, vacuum from 20.0 to 24.0psi @10:06:32.03 SS.reconfigure SSinhale.with maxSpeed: 59 @10:06:32.13 SS.seek 25ml @10:06:34.77 Stroke completed -- waiting for pressure to equalize ... @10:06:37.24 ATV.dial :waste @10:06:37.26 ARV.dial between carrier and air1 @10:06:37.37 ASV.dial :waste1 @10:06:39.82
Intake.select :close @10:06:40.01 SSV.dial :exhaust @10:06:40.79 Exhaust.select :open @10:06:40.94 SS.reconfigure SSexhale @10:06:41.01 SS.seek 0ml @10:06:49.87 Exhaust.select :close @10:06:50.02 SSV.dial CBV @10:06:50.05
CBV.dial SSV @10:06:50.87 Allowing 130s for 25.0ml of remaining 1475.0ml @10:06:50.89 Intake.select :open @10:06:53.10 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:06:53.13 SS.reconfigure SSinhale.with maxSpeed: 59 @10:06:53.22 SS.seek 25ml @10:06:58.54 Vacuum too high -- waiting up to 40s @10:07:00.36 Intake @30.8psia, vacuum from 20.0 to 24.0psi @10:07:00.38 SS.reconfigure SSinhale.with maxSpeed: 53 @10:07:00.48 SS.seek 25ml @10:07:05.66 Vacuum too high -- waiting up to 40s @10:07:07.74 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:07:07.77 SS.reconfigure SSinhale.with maxSpeed: 48 @10:07:07.86 SS.seek 25ml @10:07:13.73 Vacuum too high -- waiting up to 40s @10:07:15.79 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:07:15.81 SS.reconfigure SSinhale.with maxSpeed: 43 @10:07:15.91 SS.seek 25ml @10:07:22.76 Vacuum too high -- waiting up to 40s @10:07:24.83 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:07:24.86 SS.reconfigure SSinhale.with maxSpeed: 39 @10:07:24.95 SS.seek 25ml @10:07:33.63 Vacuum too high -- waiting up to 40s @10:07:35.73 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:07:35.76 SS.reconfigure SSinhale.with maxSpeed: 35 @10:07:35.85 SS.seek 25ml @10:07:47.14 Vacuum too high -- waiting up to 40s @10:07:49.45 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:07:49.47 SS.reconfigure SSinhale.with maxSpeed: 32 @10:07:49.56 SS.seek 25ml @10:07:49.71 Stroke completed -- waiting for pressure to equalize ... @10:07:54.17 Intake.select :close @10:07:54.36 SSV.dial :exhaust @10:07:55.15 Exhaust.select :open @10:07:55.30 SS.reconfigure SSexhale @10:07:55.37 SS.seek 0ml @10:08:04.46 Exhaust.select :close @10:08:04.60 SSV.dial CBV @10:08:04.63
CBV.dial SSV @10:08:05.45 Allowing 130s for 25.0ml of remaining 1450.0ml @10:08:05.46 Intake.select :open @10:08:07.69 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:08:07.71 SS.reconfigure SSinhale.with maxSpeed: 32 @10:08:07.80 SS.seek 25ml @10:08:26.74 Vacuum too high -- waiting up to 40s @10:08:28.55 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:08:28.57 SS.reconfigure SSinhale.with maxSpeed: 29 @10:08:28.66 SS.seek 25ml @10:08:55.42 Vacuum too high -- waiting up to 40s @10:08:57.74 SS.reconfigure SSinhale.with maxSpeed: 26 @10:08:57.82 SS.seek 25ml @10:09:12.92 Stroke completed -- waiting for pressure to equalize ... @10:09:19.14 Intake.select :close @10:09:19.33 SSV.dial :exhaust @10:09:20.12 Exhaust.select :open @10:09:20.27 SS.reconfigure SSexhale @10:09:20.34 SS.seek 0ml @10:09:29.00 Exhaust.select :close @10:09:29.14 SSV.dial CBV @10:09:29.17
CBV.dial SSV @10:09:29.96 Allowing 130s for 25.0ml of remaining 1425.0ml @10:09:29.98 Intake.select :open @10:09:32.18 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:09:32.21 SS.reconfigure SSinhale.with maxSpeed: 26 @10:09:32.30 SS.seek 25ml @10:10:15.36 Vacuum too high -- waiting up to 40s @10:10:17.18 Intake @30.8psia, vacuum from 20.0 to 24.0psi @10:10:17.21 SS.reconfigure SSinhale.with maxSpeed: 23 @10:10:17.30 SS.seek 25ml @10:10:45.18 Stroke completed -- waiting for pressure to equalize ... @10:10:51.48 Intake.select :close @10:10:51.66 SSV.dial :exhaust @10:10:52.43 Exhaust.select :open @10:10:52.57 SS.reconfigure SSexhale @10:10:52.63 SS.seek 0ml @10:11:01.64 Exhaust.select :close @10:11:01.78 SSV.dial CBV @10:11:01.81
CBV.dial SSV @10:11:02.62 Allowing 130s for 25.0ml of remaining 1400.0ml @10:11:02.64 Intake.select :open @10:11:04.85 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:11:04.87 SS.reconfigure SSinhale.with maxSpeed: 23 @10:11:04.96 SS.seek 25ml @10:12:21.05 Stroke completed -- waiting for pressure to equalize ... @10:12:28.64 Intake.select :close @10:12:28.83 SSV.dial :exhaust @10:12:29.62 Exhaust.select :open @10:12:29.76 SS.reconfigure SSexhale @10:12:29.82 SS.seek 0ml @10:12:38.82 Exhaust.select :close @10:12:38.96 SSV.dial CBV @10:12:38.99
CBV.dial SSV @10:12:39.81 Allowing 130s for 25.0ml of remaining 1375.0ml @10:12:39.83 Intake.select :open @10:12:42.56 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:12:42.58 SS.reconfigure SSinhale.with maxSpeed: 23 @10:12:42.67 SS.seek 25ml @10:13:58.74 Stroke completed -- waiting for pressure to equalize ... @10:14:07.59 Intake.select :close @10:14:07.77 SSV.dial :exhaust @10:14:08.56 Exhaust.select :open @10:14:08.70 SS.reconfigure SSexhale @10:14:08.77 SS.seek 0ml @10:14:17.86 Exhaust.select :close @10:14:18.00 SSV.dial CBV @10:14:18.03
CBV.dial SSV @10:14:18.83 Allowing 130s for 25.0ml of remaining 1350.0ml @10:14:18.86 Intake.select :open @10:14:21.13 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:14:21.15 SS.reconfigure SSinhale.with maxSpeed: 23 @10:14:21.25 SS.seek 25ml @10:15:12.49 Vacuum too high -- waiting up to 40s @10:15:14.80 Intake @30.8psia, vacuum from 20.0 to 24.0psi @10:15:14.82 SS.reconfigure SSinhale.with maxSpeed: 21 @10:15:14.92 SS.seek 25ml @10:15:43.18 Stroke completed -- waiting for pressure to equalize ... @10:15:51.37 Intake.select :close @10:15:51.56 SSV.dial :exhaust @10:15:52.34 Exhaust.select :open @10:15:52.47 SS.reconfigure SSexhale @10:15:52.53 SS.seek 0ml @10:16:01.51 Exhaust.select :close @10:16:01.65 SSV.dial CBV @10:16:01.68
CBV.dial SSV @10:16:02.50 Allowing 130s for 25.0ml of remaining 1325.0ml @10:16:02.52 Intake.select :open @10:16:04.73 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:16:04.75 SS.reconfigure SSinhale.with maxSpeed: 21 @10:16:04.85 SS.seek 25ml @10:17:26.58 Vacuum too high -- waiting up to 40s @10:17:29.20 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:17:29.23 SS.reconfigure SSinhale.with maxSpeed: 19 @10:17:29.32 SS.seek 25ml @10:17:31.79 Stroke completed -- waiting for pressure to equalize ... @10:17:39.37 Intake.select :close @10:17:39.54 SSV.dial :exhaust @10:17:40.33 Exhaust.select :open @10:17:40.47 SS.reconfigure SSexhale @10:17:40.54 SS.seek 0ml @10:17:49.26 Exhaust.select :close @10:17:49.39 SSV.dial CBV @10:17:49.42
CBV.dial SSV @10:17:50.25 Allowing 130s for 25.0ml of remaining 1300.0ml @10:17:50.27 Intake.select :open @10:17:52.47 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:17:52.50 SS.reconfigure SSinhale.with maxSpeed: 19 @10:17:52.59 SS.seek 25ml @10:19:25.09 Stroke completed -- waiting for pressure to equalize ... @10:19:36.37 Intake.select :close @10:19:36.55 SSV.dial :exhaust @10:19:37.34 Exhaust.select :open @10:19:37.47 SS.reconfigure SSexhale @10:19:37.53 SS.seek 0ml @10:19:46.37 Exhaust.select :close @10:19:46.51 SSV.dial CBV @10:19:46.54
CBV.dial SSV @10:19:47.34 Allowing 130s for 25.0ml of remaining 1275.0ml @10:19:47.35 Intake.select :open @10:19:49.57 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:19:49.59 SS.reconfigure SSinhale.with maxSpeed: 19 @10:19:49.68 SS.seek 25ml @10:20:27.58 Vacuum too high -- waiting up to 40s @10:20:29.94 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:20:29.96 SS.reconfigure SSinhale.with maxSpeed: 17 @10:20:30.06 SS.seek 25ml @10:21:31.99 Stroke completed -- waiting for pressure to equalize ... @10:21:43.98 Intake.select :close @10:21:44.16 SSV.dial :exhaust @10:21:44.94 Exhaust.select :open @10:21:45.09 SS.reconfigure SSexhale @10:21:45.16 SS.seek 0ml @10:21:54.19 Exhaust.select :close @10:21:54.33 SSV.dial CBV @10:21:54.36
CBV.dial SSV @10:21:55.17 Allowing 130s for 25.0ml of remaining 1250.0ml @10:21:55.19 Intake.select :open @10:21:57.39 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:21:57.41 SS.reconfigure SSinhale.with maxSpeed: 17 @10:21:57.51 SS.seek 25ml @10:22:42.02 Vacuum too high -- waiting up to 40s @10:22:44.83 Intake @30.8psia, vacuum from 20.0 to 24.0psi @10:22:44.85 SS.reconfigure SSinhale.with maxSpeed: 15 @10:22:44.94 SS.seek 25ml @10:23:52.16 Stroke completed -- waiting for pressure to equalize ... @10:24:05.20 SS.reconfigure SSconfig @10:24:05.26 SS.hold @10:24:05.40 Intake.select :close @10:24:05.58 ShallowSampler::Clogged in MAIN -- progress too slow after 275.0ml @10:24:05.60 SSV.dial :exhaust @10:24:06.41 Exhaust.select :open @10:24:06.56 SS.reconfigure SSexhale @10:24:06.63 SS.seek 0ml @10:24:15.75 Exhaust.select :close @10:24:15.88 Sampled 275.0ml @10:24:15.90 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:24:15.92 Intake.select :close @10:24:16.03 CSV.dial :waste @10:24:16.11 CS.seek 0ml @10:24:16.25 CSV.dial :air @10:24:16.29 SSV.dial CBV,avoiding: :exhaust @10:24:16.34
CBV.dial SSV @10:24:17.12 CS.seek 10ml @10:24:18.25
CTV.dial :air @10:24:19.03 delay 15 seconds @10:24:34.06 SSV.dial :spit,avoiding: :exhaust @10:24:35.42 CTV.dial CSV,avoiding: :intake @10:24:35.46
SS.seek 0ml @10:24:35.62 SSV.dial CBV,avoiding: :exhaust @10:24:39.86 CBV.dial between SSV and waste2 @10:24:40.29 CTV.dial CSV,avoiding: :air @10:24:40.33
CSV.dial CTV @10:24:40.82 CS.reconfigure CSpressurize @10:24:40.89 CS.seek 0ml @10:25:06.88 BubblePoint: Intake @70.5psia, Outlet @14.4psia @10:25:06.89 CBV.dial SSV @10:25:07.28 SS.reconfigure SSconfig @10:25:07.36 SS.seek 10ml @10:25:12.89 delay 15 seconds @10:25:28.03 Evacuated: Intake @36.8psia, Outlet @0.9psia @10:25:28.05 SSV.dial :spit,avoiding: :exhaust @10:25:29.42 SS.reconfigure SSexhale @10:25:29.49 SS.seek 0ml @10:25:34.60 SS.reconfigure SSfast @10:25:34.68 SS.seek 5ml @10:25:37.20 SSV.dial CBV,avoiding: :exhaust @10:25:37.24
CBV.dial SSV @10:25:38.58 CTV.dial :air,avoiding: :intake @10:25:39.92 CSV.dial CTV @10:25:39.99
SS.seek 10ml @10:25:40.97 CSV.dial :air @10:25:41.44 CS.reconfigure CSfast @10:25:41.51 CS.seek 0ml @10:25:42.33 CS.coast @10:25:42.66
SSV.dial :spit,avoiding: :exhaust @10:25:43.99 SS.reconfigure SSexhale @10:25:44.07 SS.seek 0ml @10:25:48.96 SS.reconfigure SSfast @10:25:49.04 SS.seek 5ml @10:25:51.72 SSV.dial CBV,avoiding: :exhaust @10:25:51.76
CBV.dial SSV @10:25:54.62 SSV.dial :spit,avoiding: :exhaust @10:25:55.95 SS.reconfigure SSconfig @10:25:56.04 SS.seek 0ml @10:25:59.20 SS.coast @10:25:59.27 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:25:59.28 Intake.select :close @10:25:59.40 CSV.dial :waste @10:25:59.55 SSV.dial CBV,avoiding: :exhaust @10:25:59.58
CBV.dial SSV @10:26:00.22 CS.seek 0ml @10:26:00.33 CSV.dial :air @10:26:00.94
CTV.dial :air @10:26:00.96 delay 15 seconds @10:26:01.07 CS.seek 10ml @10:26:16.00
SSV.dial :spit,avoiding: :exhaust @10:26:17.35 CTV.dial CSV,avoiding: :intake @10:26:17.39
SS.reconfigure SSexhale @10:26:17.53 SS.seek 0ml @10:26:17.62 SSV.dial CBV,avoiding: :exhaust @10:26:23.48 CBV.dial between SSV and waste2 @10:26:23.93 CSV.dial between air and CTV @10:26:24.28 CS.seek 8.232ml @10:26:29.26 CTV.dial CSV,avoiding: :air @10:26:29.29
CSV.dial CTV @10:26:29.67 CS.reconfigure CSpressurize @10:26:29.74 CS.seek 0ml @10:26:51.46 CSV.dial :air @10:26:51.94 CS.reconfigure CSfast @10:26:52.02 CS.seek 10ml @10:27:14.14 CSV.dial between air and CTV @10:27:14.50 CS.seek 3.049ml @10:27:30.28 CTV.dial CSV,avoiding: :air @10:27:30.31
CSV.dial CTV @10:27:30.67 CS.reconfigure CSpressurize @10:27:30.74 CS.seek 0ml @10:27:38.13 BubblePoint: Intake @69.6psia, Outlet @14.0psia @10:27:38.14 CBV.dial SSV @10:27:38.53 SS.reconfigure SSconfig @10:27:38.61 SS.seek 10ml @10:27:44.05 delay 15 seconds @10:27:59.20 Evacuated: Intake @39.9psia, Outlet @15.3psia @10:27:59.21 SSV.dial :spit,avoiding: :exhaust @10:28:00.56 SS.reconfigure SSexhale @10:28:00.64 SS.seek 0ml @10:28:05.53 SS.reconfigure SSfast @10:28:05.61 SS.seek 5ml @10:28:08.39 SSV.dial CBV,avoiding: :exhaust @10:28:08.43
CBV.dial SSV @10:28:09.77 CTV.dial :air,avoiding: :intake @10:28:11.10 CSV.dial CTV @10:28:11.16
SS.seek 10ml @10:28:12.14 CSV.dial :air @10:28:12.61 CS.reconfigure CSfast @10:28:12.69 CS.seek 0ml @10:28:13.78
SSV.dial :spit,avoiding: :exhaust @10:28:14.85 CS.coast @10:28:15.14
SS.reconfigure SSexhale @10:28:15.21 SS.seek 0ml @10:28:20.12 SS.reconfigure SSfast @10:28:20.20 SS.seek 5ml @10:28:23.43 SSV.dial CBV,avoiding: :exhaust @10:28:23.46
CBV.dial SSV @10:28:25.83 SSV.dial :spit,avoiding: :exhaust @10:28:27.16 SS.reconfigure SSconfig @10:28:27.24 SS.seek 0ml @10:28:30.38 SS.coast @10:28:30.45 Lysing: Adding 1.5ml lysis @10:28:30.46 CBV.dial between CSV and waste1,avoiding: :DBV @10:28:32.00 CTV.dial CSV @10:28:33.33 CSV.dial CSR @10:28:34.14 Pulling 1ml of CSR.lysis @10:28:34.16 CSR.select :lysis @10:28:34.32 CS.reconfigure CSconfig @10:28:34.39 CS.seek 1ml @10:28:37.96 delay 5 seconds @10:28:42.99 CSR.select :air @10:28:43.18 CS.seek 6ml @10:28:56.87 delay 2 seconds @10:28:58.89 CSV.dial :waste1 @10:29:00.19 CS.reconfigure CSfast @10:29:00.27 CS.seek :empty @10:29:14.02 CSV.dial CSR @10:29:15.38 Pulling 1.5ml of CSR.lysis @10:29:15.40 CSR.select :lysis @10:29:15.56 CS.reconfigure CSconfig @10:29:15.63 CS.seek 1.5ml @10:29:20.49 delay 5 seconds @10:29:25.52 CSR.select :air @10:29:25.72 CS.seek 4.5ml @10:29:34.43 delay 2 seconds @10:29:36.45 CSV.dial CTV @10:29:36.94 CS.seek 1.275ml @10:29:46.19 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @10:29:46.21 CH.seek 85C,8:00 @10:32:39.86 CH.hold 5:00,85C @10:37:40.03 Collection Puck Heater at 85C after lysing @10:37:40.06 CS.seek 0ml @10:37:44.55 CTV.dial between spare and air,avoiding: :intake @10:37:45.63 CSV.dial :air @10:37:46.68 CS.seek 5ml @10:38:00.44 CSV.dial CTV @10:38:00.92 delay 2 seconds @10:38:02.95 CSV.dial :air @10:38:03.44 delay 2 seconds @10:38:05.46 CSV.dial CTV @10:38:05.95 delay 2 seconds @10:38:07.98 CSV.dial :air @10:38:08.47 delay 2 seconds @10:38:10.50 CS.seek 0ml @10:38:24.47 Recovering Lysate into Collection syringe @10:38:24.48 CSV.dial CBV @10:38:25.53 CBV.dial CSV @10:38:25.96 CS.seek 5.001ml @10:38:39.72 delay 5 seconds @10:38:44.75 CTV.dial :air @10:38:45.31 CS.seek 7ml @10:38:51.35 delay 5 seconds @10:38:56.38 CSV.dial :air2 @10:38:56.86 CS.seek 10ml @10:39:05.69 CSV.dial between air and CTV,avoiding: CBV @10:39:06.85 Lysing Complete @10:39:06.95 Storing sh1 puck in Collection Clamp to tube 1 @10:39:07.16 SC.reconfigure SCtight @10:39:07.24 SC.seek 1 @10:39:09.38 SC.reconfigure SCloose @10:39:09.63 Elbow.reconfigure TightElbow @10:39:09.71 Elbow.seek 1 @10:39:11.69 Forearm.reconfigure TightForearm @10:39:11.77 Forearm.seek 1 + 700 counts @10:39:12.86 SE.reconfigure SEpick @10:39:12.96 SE.seek beamBreak @10:39:15.50 SE.configure SEconfig @10:39:15.69 Elbow.reconfigure LimpElbow @10:39:15.77 Forearm.reconfigure LooseForearm @10:39:15.84 Forearm.seek 1 @10:39:16.18 Elbow.hold @10:39:16.27 Elbow.reconfigure TightElbow @10:39:16.40 SE.seek 5.07 Pucks below Top Plate @10:39:16.88 Forearm.force -20 @10:39:16.95 Forearm.seek 1 + 321 counts @10:39:17.33 Forearm.reconfigure TightForearm @10:39:17.40 Forearm.seek 1 + 700 counts @10:39:17.76 SE.seek 6.07 Pucks below Top Plate @10:39:18.52 Forearm.reconfigure LooseForearm @10:39:18.60 Forearm.seek :retract @10:39:19.57 Elbow.seek CC @10:39:21.27 Elbow.reconfigure LooseElbow @10:39:21.40 Forearm.seek CC @10:39:22.48 Hand.close @10:39:23.07 CC.seek :open @10:39:28.13 Forearm.seek :retract @10:39:29.18 Elbow.reconfigure TightElbow @10:39:29.26 Elbow.seek 1 @10:39:31.38 Elbow.reconfigure LimpElbow @10:39:31.46 Forearm.seek 1 @10:39:32.51 Elbow.hold @10:39:32.61 Elbow.reconfigure TightElbow @10:39:32.68 Hand.open @10:39:33.45 Forearm.force -20 @10:39:33.52 Forearm.seek 1 + 347 counts @10:39:33.87 Forearm.reconfigure TightForearm @10:39:33.94 Forearm.seek 1 + 700 counts @10:39:34.34 SE.seek :down @10:39:35.49
Forearm.reconfigure LooseForearm @10:39:35.57 Forearm.seek :retract @10:39:36.52 SE.coast @10:39:36.60
SC.coast @10:39:36.65 Tranferring lysate to PRV @10:39:36.66 CSV.dial :air @10:39:37.01 CS.seek 10ml @10:39:37.10 CSV.dial between air and CTV @10:39:37.44 AIV.dial :bypass @10:39:37.57 Beginning samplePassC @10:39:37.58 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:39:37.69 Hand.open @10:39:38.09 Elbow.seek FlushPuck::Garage @10:39:40.12 Elbow.reconfigure LooseElbow @10:39:40.26 Forearm.seek :garage @10:39:41.28 Hand.close @10:39:42.08 Forearm.seek :retract @10:39:43.12 Elbow.reconfigure TightElbow @10:39:43.19 Elbow.seek CC @10:39:46.15 Elbow.reconfigure LooseElbow @10:39:46.28 Forearm.reconfigure TightForearm @10:39:46.35 Forearm.seek CC @10:39:47.66 CC.seek :closed @10:39:51.94 Collection Clamp onSpring/offSpring for 6/32 samples, on/off torque 1.81 @10:39:52.05 Hand.open @10:39:52.77 Forearm.reconfigure LooseForearm @10:39:52.84 Forearm.seek :retract @10:39:53.86 AWV.select :air @10:39:53.87 AIV.dial :bypass @10:39:53.90 PSV.dial PSR @10:39:55.26 Pulling 1.5ml of PSR.flush @10:39:55.27 PSR.select :flush @10:39:55.97 PS.seek 1.5ml @10:40:11.95 delay 5 seconds @10:40:16.98 PSV.dial :waste1 @10:40:17.48 PS.seek 0ml @10:40:33.49 PSV.dial PSR @10:40:33.97 PSR.select :air @10:40:34.12 PS.seek 2.5ml @10:41:00.34 PRV.dial CBV @10:41:00.37
CBV.dial PRV @10:41:01.22 PSV.dial :mixloop @10:41:02.24 CTV.dial :air,avoiding: :intake @10:41:02.26 PTV.dial :PRVmixing @10:41:02.40 PS.seek 0ml @10:41:28.61 PSV.dial :air @10:41:29.09 PS.seek 2.5ml @10:41:55.30 PSV.dial :mixloop @10:41:55.79 PS.seek 0ml @10:42:22.02 PSV.dial :air @10:42:22.51 PS.seek 2.5ml @10:42:48.61 PSV.dial :mixloop @10:42:49.11 PS.seek 0ml @10:43:15.24 Garaging FlushPuck in Collection Clamp @10:43:15.35 Elbow.reconfigure TightElbow @10:43:15.42 Elbow.seek CC @10:43:15.74 Elbow.reconfigure LooseElbow @10:43:15.86 Forearm.seek CC @10:43:16.94 Hand.close @10:43:17.58 CC.seek :open @10:43:21.98 Forearm.seek :retract @10:43:23.08 Elbow.reconfigure TightElbow @10:43:23.15 Elbow.seek :garage @10:43:26.34 Elbow.reconfigure LooseElbow @10:43:26.46 Forearm.reconfigure TightForearm @10:43:26.53 Forearm.seek :garage @10:43:27.71 Hand.open @10:43:28.40 Forearm.reconfigure LooseForearm @10:43:28.47 Forearm.seek :retract @10:43:29.53 Connecting PS to CS to transfer sample @10:43:29.54 CBV.dial AIV @10:43:29.57 AIV.dial :bypass @10:43:29.59 PSV.dial :mixloop @10:43:29.62 PTV.dial :PRVmixing @10:43:29.65 PRV.dial AIV @10:43:29.69 CTV.dial :air,avoiding: :intake @10:43:29.72 Loading lyfil puck from tube 2 into Collection Clamp @10:43:30.01 SC.reconfigure SCtight @10:43:30.08 SC.seek 2 @10:43:32.23 SC.reconfigure SCloose @10:43:32.48 Elbow.reconfigure TightElbow @10:43:32.55 Elbow.seek 2 @10:43:34.70 Forearm.reconfigure TightForearm @10:43:34.77 Forearm.seek 2 + 700 counts @10:43:35.82 SE.reconfigure SEpick @10:43:35.92 SE.seek beamBreak @10:43:37.51 SE.configure SEconfig @10:43:37.70 Elbow.seek 2 @10:43:37.92 Forearm.seek 2 + 300 counts @10:43:38.16 Forearm.reconfigure LooseForearm @10:43:38.23 Hand.close @10:43:39.48 SE.seek :down @10:43:39.74
Forearm.seek :retract @10:43:40.72 Elbow.seek CC @10:43:41.04 SE.coast @10:43:42.42
Elbow.reconfigure LooseElbow @10:43:42.55 Forearm.reconfigure TightForearm @10:43:42.62 Forearm.seek CC @10:43:43.99 CC.seek :closed @10:43:48.26 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.83 @10:43:48.27 Hand.open @10:43:49.03 Forearm.reconfigure LooseForearm @10:43:49.10 Forearm.seek :retract @10:43:50.18 SC.coast @10:43:50.24 CTV.dial CSV,avoiding: :intake @10:43:51.57 CSV.dial CTV @10:43:51.91 CS.seek 5ml @10:44:05.70 delay 1:20 @10:45:25.78 CS.seek 2.5ml @10:45:33.23 delay 40 seconds @10:46:13.27 CS.seek 0ml @10:46:20.79 delay 1:00 @10:47:20.82 PSV.dial :air @10:47:21.30 PS.seek 2.5ml @10:47:47.49 PSV.dial :mixloop @10:47:47.98 delay 3 seconds @10:47:51.01 PSV.dial :air @10:47:51.46 delay 2 seconds @10:47:53.48 PSV.dial :mixloop @10:47:53.95 delay 3 seconds @10:47:56.98 PSV.dial :waste1 @10:47:58.30 PS.seek :empty @10:48:24.43 CBV.dial :waste1 @10:48:24.92 delay 5 seconds @10:48:29.95 CBV.dial AIV @10:48:30.45 delay 10 seconds @10:48:40.48 CBV.dial :waste1 @10:48:40.97 delay 10 seconds @10:48:51.00 CBV.dial AIV @10:48:51.48 delay 10 seconds @10:49:01.51 CTV.dial CSV,avoiding: :intake @10:49:01.53 CBV.dial between AIV and waste1 @10:49:01.90 CSV.dial :air @10:49:02.38 CS.seek 10ml @10:49:28.89 CSV.dial CTV @10:49:29.38 CS.seek 0ml @10:49:55.92 CBV.dial :waste1 @10:49:56.27 delay 10 seconds @10:50:06.30 CTV.dial :air,avoiding: :intake @10:50:07.69 Storing lyfil puck in Collection Clamp to tube 1 @10:50:07.89 SC.reconfigure SCtight @10:50:07.95 SC.seek 1 @10:50:10.06 SC.reconfigure SCloose @10:50:10.32 Elbow.reconfigure TightElbow @10:50:10.39 Elbow.seek 1 @10:50:12.29 Forearm.reconfigure TightForearm @10:50:12.36 Forearm.seek 1 + 700 counts @10:50:13.48 SE.reconfigure SEpick @10:50:13.59 SE.seek beamBreak @10:50:16.50 SE.configure SEconfig @10:50:16.77 Elbow.reconfigure LimpElbow @10:50:16.84 Forearm.reconfigure LooseForearm @10:50:16.91 Forearm.seek 1 @10:50:17.29 Elbow.hold @10:50:17.39 Elbow.reconfigure TightElbow @10:50:17.52 SE.seek 6.07 Pucks below Top Plate @10:50:18.01 Forearm.force -20 @10:50:18.07 Forearm.seek 1 + 310 counts @10:50:18.40 Forearm.reconfigure TightForearm @10:50:18.47 Forearm.seek 1 + 700 counts @10:50:18.82 SE.seek 7.07 Pucks below Top Plate @10:50:19.46 Forearm.reconfigure LooseForearm @10:50:19.53 Forearm.seek :retract @10:50:20.50 Elbow.seek CC @10:50:22.15 Elbow.reconfigure LooseElbow @10:50:22.29 Forearm.seek CC @10:50:23.34 Hand.close @10:50:23.97 CC.seek :open @10:50:28.49 Forearm.seek :retract @10:50:29.56 Elbow.reconfigure TightElbow @10:50:29.63 Elbow.seek 1 @10:50:31.63 Elbow.reconfigure LimpElbow @10:50:31.71 Forearm.seek 1 @10:50:32.73 Elbow.hold @10:50:32.83 Elbow.reconfigure TightElbow @10:50:32.90 Hand.open @10:50:33.72 Forearm.force -20 @10:50:33.79 Forearm.seek 1 + 349 counts @10:50:34.11 Forearm.reconfigure TightForearm @10:50:34.18 Forearm.seek 1 + 700 counts @10:50:34.60 SE.seek :down @10:50:35.74
Forearm.reconfigure LooseForearm @10:50:35.81 Forearm.seek :retract @10:50:36.81 SE.coast @10:50:36.87
SC.coast @10:50:36.93 PSV.dial :air @10:50:38.00 PS.seek 0.499ml @10:50:43.87 delay 3 seconds @10:50:46.89 PSV.dial :mixloop @10:50:47.35 PRV.dial :air @10:50:47.84 delay 3 seconds @10:50:50.87 PRV.dial CBV @10:50:51.34 CBV.dial AIV @10:50:51.83 PRV.dial between CBV and air @10:50:52.18 PRV.dial :air @10:50:52.60 PS.reconfigure PSslow @10:50:52.67 PS.seek 0.599ml @10:50:55.28 PRV.dial :waste2 @10:50:56.06 PS.reconfigure PSconfig @10:50:56.13 PS.seek :empty @10:51:03.00 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:51:03.09 Hand.open @10:51:04.00 Elbow.seek FlushPuck::Garage @10:51:06.06 Elbow.reconfigure LooseElbow @10:51:06.19 Forearm.seek :garage @10:51:07.20 Hand.close @10:51:07.97 Forearm.seek :retract @10:51:09.06 Elbow.reconfigure TightElbow @10:51:09.13 Elbow.seek CC @10:51:12.06 Elbow.reconfigure LooseElbow @10:51:12.19 Forearm.reconfigure TightForearm @10:51:12.27 Forearm.seek CC @10:51:13.61 CC.seek :closed @10:51:17.89 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.80 @10:51:17.99 Hand.open @10:51:18.66 Forearm.reconfigure LooseForearm @10:51:18.73 Forearm.seek :retract @10:51:19.82 Clearing PML for modified lysate @10:51:19.83 CTV.dial :air,avoiding: :intake @10:51:19.85 PRV.dial :waste2 @10:51:19.88 PTV.dial :PRV,avoiding: :puck @10:51:20.68 delay 2 seconds @10:51:22.70 PTV.dial :PRVmixing,avoiding: :puck @10:51:23.48 delay 2 seconds @10:51:25.50 PSV.dial :PML @10:51:25.52 delay 2 seconds @10:51:27.55 PRV.dial :air @10:51:28.29 delay 2 seconds @10:51:30.37 PS.seek 1.499ml @10:51:46.37 delay 5 seconds @10:51:51.41 PS.seek 2.5ml @10:52:02.50 PSV.dial :air @10:52:02.98 PS.seek 0ml @10:52:29.15 mixing lysate with speDil @10:52:29.16 PSV.dial :PML @10:52:29.67 CBV.dial :PRV @10:52:29.68 PRV.dial :air @10:52:29.72 delay 2 seconds @10:52:31.75 PRV.dial :speDil @10:52:32.30 delay 2 seconds @10:52:34.40 PS.reconfigure PSslow1 @10:52:34.49 PS.seek 0.225ml @10:52:58.09 delay 5 seconds @10:53:03.12 PRV.dial CBV @10:53:03.90 delay 2 seconds @10:53:05.98 PS.seek 0.475ml @10:53:31.84 delay 5 seconds @10:53:36.87 PRV.dial :air @10:53:37.37 delay 2 seconds @10:53:39.40 PS.seek 0.965ml @10:54:29.34 delay 10 seconds @10:54:39.37 PRV.dial CBV @10:54:39.86 CTV.dial :air,avoiding: :intake @10:54:39.88 PSV.dial :air @10:54:40.37 PS.reconfigure PSconfig @10:54:40.45 PS.seek 2.5ml @10:54:56.87 delay 3 seconds @10:54:59.89 PSV.dial PTV @10:55:00.64 PTV.dial :PRV,avoiding: :puck @10:55:01.42 PS.seek 0ml @10:55:27.69 PSV.dial :air @10:55:28.99 PS.seek 2.5ml @10:55:55.16 delay 3 seconds @10:55:58.18 PSV.dial PTV @10:55:58.92 PTV.dial :PRV,avoiding: :puck @10:55:58.95 PS.seek 0ml @10:56:25.16 Pushing modified lysate into AIV Injection Loop @10:56:25.17 CBV.dial AIV @10:56:25.21 AIV.dial :bypass @10:56:25.24 PRV.dial CBV @10:56:25.27 PSV.dial :air @10:56:26.04 PS.seek 2.5ml @10:56:52.16 delay 3 seconds @10:56:55.18 PSV.dial :PML @10:56:55.66 PTV.dial :PRVmixing,avoiding: :puck @10:56:56.49 PS.reconfigure PSslow1 @10:56:56.56 PS.seek 1.74ml @10:58:13.88 delay 5 seconds @10:58:18.91 AIV.dial :sample @10:58:19.51 AWV.select :waste @10:58:19.70 PS.reconfigure PSslow2 @10:58:19.77 PS.seek 1.22ml @11:00:07.00 delay 15 seconds @11:00:22.03 AIV.dial :bypass @11:00:22.62 PS.reconfigure PSconfig @11:00:22.69 PS.seek 0ml @11:00:28.27 Session Ended @11:00:35.82
PSV.dial :air @11:00:36.30 PS.seek 2.5ml @11:01:02.38 PSV.dial :PML @11:01:02.84 PS.seek 0ml @11:01:29.04 AIV.dial :coilToMFB @11:01:29.05 AWV.select :air @11:01:29.22 Extracting Nucleic Acid from AIV @11:01:29.25
Beginning samplePassD @11:01:29.28 SPE.hold 2:46:40,55C @11:01:29.37 ASV.dial :SPEdelivery @11:01:29.45
AWV.select :air @11:01:29.47 AIV.dial :bypass @11:01:29.48 PSV.dial PSR @11:01:29.78 delay 20 seconds @11:01:30.60
Pulling 1.5ml of PSR.killMFB @11:01:30.61 PSR.select :killMFB @11:01:30.74 PS.seek 1.5ml @11:01:46.79 delay 5 seconds @11:01:49.81 primeLysateINT @11:01:49.82 AWV.select :air @11:01:49.83 AIV.dial :coilToMFB @11:01:49.85 ATV.dial AIV @11:01:51.49 AS.reconfigure ASslow1 @11:01:51.54 AS.seek 0.299ml @11:01:51.81
PSV.dial :waste1 @11:01:52.30 PS.seek 0ml @11:02:08.29 PSV.dial PSR @11:02:08.77 PSR.select :air @11:02:08.89 PS.seek 2.5ml @11:02:35.10 PRV.dial CBV @11:02:35.13
CBV.dial PRV @11:02:35.14 PSV.dial :mixloop @11:02:36.16 CTV.dial :air,avoiding: :intake @11:02:36.17 PTV.dial :PRVmixing @11:02:36.19 PS.seek 0ml @11:02:52.14 delay 10 seconds @11:03:02.17 ATV.dial :air2 @11:03:02.29
PSV.dial PSR @11:03:02.98 AS.seek 0.329ml @11:03:03.40
Pulling 1.5ml of PSR.flush @11:03:03.41 PSR.select :flush @11:03:03.54 PS.seek 1.5ml @11:03:09.28 ATV.dial :waste2 @11:03:09.81 AS.reconfigure ASnorm @11:03:09.86 AS.seek :empty @11:03:19.54
delay 5 seconds @11:03:24.57 PSV.dial :waste1 @11:03:25.05 PS.seek 0ml @11:03:26.87 Lysate primed! @11:03:26.88 AFV.select :holdingCoil @11:03:26.89 ATV.dial :air2 @11:03:27.49 AS.reconfigure ASslow1 @11:03:27.54 AS.seek 0.069ml @11:03:41.50
PSV.dial PSR @11:03:41.91 delay 3 seconds @11:03:42.12
PSR.select :air @11:03:42.25 PS.seek 2.5ml @11:03:44.93 ATV.dial :AIV @11:03:45.75 AS.seek 0.469ml @11:04:08.35 PRV.dial CBV @11:04:08.38
CBV.dial PRV @11:04:08.39 PSV.dial :mixloop @11:04:09.43 CTV.dial :air,avoiding: :intake @11:04:09.44 PTV.dial :PRVmixing @11:04:09.47 PS.seek 0ml @11:04:35.67 PSV.dial PSR @11:04:36.76 Pulling 1.5ml of PSR.flush @11:04:36.77 PSR.select :flush @11:04:36.90 PS.seek 1.5ml @11:04:52.88 delay 5 seconds @11:04:57.91 PSV.dial :waste1 @11:04:58.39 PS.seek 0ml @11:05:06.44 delay 5 seconds @11:05:11.53 Solid Phase Extraction Heater at 49.4C @11:05:11.54 ATV.dial ASV @11:05:11.92 ASV.dial :SPEdelivery @11:05:12.00 AS.seek 0.041ml @11:05:14.48
PSV.dial PSR @11:05:14.95 PSR.select :air @11:05:15.07 PS.seek 2.5ml @11:05:41.13 PRV.dial CBV @11:05:41.16
CBV.dial PRV @11:05:41.17 PSV.dial :mixloop @11:05:42.18 CTV.dial :air,avoiding: :intake @11:05:42.19 PTV.dial :PRVmixing @11:05:42.21 PS.seek 0ml @11:06:08.32 PSV.dial PSR @11:06:09.38 Pulling 1.5ml of PSR.flush @11:06:09.39 PSR.select :flush @11:06:09.52 PS.seek 1.5ml @11:06:25.48 delay 5 seconds @11:06:30.51 PSV.dial :waste1 @11:06:31.02 PS.seek 0ml @11:06:38.35 delay 10 seconds @11:06:47.04
PSV.dial PSR @11:06:47.62 PSR.select :air @11:06:47.75 PS.seek 2.5ml @11:06:48.37 rinseLysateLineINT @11:06:48.38 AFV.select :carrierSolution @11:06:48.48 delay 2 seconds @11:06:50.51 AS.reconfigure ASconfig @11:06:50.56 AS.seek 1ml @11:07:13.86 PRV.dial CBV @11:07:13.88
CBV.dial PRV @11:07:13.91 PSV.dial :mixloop @11:07:14.92 CTV.dial :air,avoiding: :intake @11:07:14.93 PTV.dial :PRVmixing @11:07:14.95 PS.reconfigure PSslow @11:07:15.00 PS.seek 1.2ml @11:07:41.79 PSV.dial :waste1 @11:07:43.07 PS.reconfigure PSconfig @11:07:43.12 PS.seek 0ml @11:07:45.97 delay 1 second @11:07:46.99 AFV.select :holdingCoil @11:07:47.10 delay 1 second @11:07:48.13 ATV.dial AIV @11:07:48.67 AIV.dial :coilToMFB @11:07:48.68 AWV.select :waste @11:07:48.80 AS.reconfigure ASslow0 @11:07:48.84 AS.seek 0ml @11:07:56.07
PSV.dial :air @11:07:57.08 PS.seek 2.5ml @11:08:23.20 PSV.dial :mixloop @11:08:23.64 PS.reconfigure PSslow @11:08:23.69 PS.seek 0ml @11:09:14.83 PSV.dial :air @11:09:15.28 PS.reconfigure PSconfig @11:09:15.33 PS.seek 2.5ml @11:09:29.75 ATV.dial :air2 @11:09:30.40 AS.reconfigure ASslow1 @11:09:30.45 AS.seek 0.05ml @11:09:40.79 ATV.dial :AIV @11:09:41.52
PSV.dial :mixloop @11:09:42.18 AS.seek 0.01ml @11:09:42.52
PS.reconfigure PSslow @11:09:42.56 PS.seek 0ml @11:09:50.50 ATV.dial :waste @11:09:52.05 AS.seek :empty @11:09:54.47 ethanol wash @11:09:54.48 ATV.dial :air2 @11:09:55.71 AS.reconfigure ASslow2 @11:09:55.76 AS.seek 0.009ml @11:10:06.10 delay 3 seconds @11:10:09.13 Pulling 0.4ml of Ethanol Wash @11:10:09.14 ATV.dial :ethanol @11:10:09.95 AS.reconfigure ASslow1 @11:10:10.00 AS.seek 0.409ml @11:10:33.61
Cleaning Collection Syringe @11:10:33.62 CSV.dial CSR @11:10:34.13 Pulling 4ml of CSR.flush @11:10:34.14 CSR.select :flush @11:10:34.27 CS.seek 4ml @11:10:45.51 delay 5 seconds @11:10:50.54 CSR.select :air @11:10:50.67 CS.seek 10ml @11:11:07.04 CSV.dial CTV @11:11:07.07
CTV.dial CSV @11:11:08.46 CBV.dial :waste1 @11:11:08.96 CS.seek 0ml @11:11:30.76 delay 3 seconds @11:11:33.79 ATV.dial :air1 @11:11:34.19 delay 3 seconds @11:11:35.54
CSV.dial CSR @11:11:36.06 Pulling 4ml of CSR.flush @11:11:36.07 CSR.select :flush @11:11:36.20 CS.seek 4ml @11:11:37.27 AS.seek 0.434ml @11:11:42.55 delay 3 seconds @11:11:45.58 ATV.dial :ethanol @11:11:46.18 AS.seek 0.419ml @11:11:47.45
delay 5 seconds @11:11:49.47 delay 3 seconds @11:11:52.48
CSR.select :air @11:11:52.53 ATV.dial ASV @11:11:52.60
CS.seek 10ml @11:11:53.79 AS.seek 0.092ml @11:12:08.92 CSV.dial CTV @11:12:08.96
CTV.dial CSV @11:12:09.43 CBV.dial :waste1 @11:12:09.45 CS.seek 0ml @11:12:36.07 Garaging FlushPuck in Collection Clamp @11:12:36.15 Elbow.reconfigure TightElbow @11:12:36.20 Elbow.seek CC @11:12:36.47 Elbow.reconfigure LooseElbow @11:12:36.58 Forearm.seek CC @11:12:37.59 Hand.close @11:12:38.26 CC.seek :open @11:12:42.64 Forearm.seek :retract @11:12:43.62 Elbow.reconfigure TightElbow @11:12:43.67 Elbow.seek :garage @11:12:46.86 Elbow.reconfigure LooseElbow @11:12:46.96 Forearm.reconfigure TightForearm @11:12:47.01 Forearm.seek :garage @11:12:48.17 Hand.open @11:12:48.83 Forearm.reconfigure LooseForearm @11:12:48.88 Forearm.seek :retract @11:12:49.88 Whole Cell Archive sampling 1500ml @11:12:49.90 Loading wcr puck from tube 2 into Collection Clamp @11:12:50.15 SC.reconfigure SCtight @11:12:50.20 SC.seek 2 @11:12:52.34 SC.reconfigure SCloose @11:12:53.12 Elbow.reconfigure TightElbow @11:12:53.18 Elbow.seek 2 @11:12:55.24 Forearm.reconfigure TightForearm @11:12:55.29 Forearm.seek 2 + 700 counts @11:12:56.36 SE.reconfigure SEpick @11:12:56.44 SE.seek beamBreak @11:12:58.34 SE.configure SEconfig @11:12:58.52 Elbow.seek 2 @11:12:58.72 Forearm.seek 2 + 300 counts @11:12:58.98 Forearm.reconfigure LooseForearm @11:12:59.03 Hand.close @11:12:59.65 SE.seek :down @11:12:59.89 delay 3 seconds @11:12:59.93 Forearm.seek :retract @11:13:00.90 Elbow.seek CC @11:13:01.36 SE.coast @11:13:02.52 Elbow.reconfigure LooseElbow @11:13:02.63 Forearm.reconfigure TightForearm @11:13:02.68 Forearm.seek CC @11:13:02.94 ATV.dial :waste2 @11:13:03.66 AS.reconfigure ASconfig @11:13:03.71 AS.seek :empty @11:13:04.12 CC.seek :closed @11:13:08.39 Collection Clamp onSpring/offSpring for 6/30 samples, on/off torque 1.87 @11:13:08.41 Hand.open @11:13:09.09 Forearm.reconfigure LooseForearm @11:13:09.14 Forearm.seek :retract @11:13:09.37 ATV.dial :air2 @11:13:09.76 AS.reconfigure ASslow1 @11:13:09.81 AS.seek 1ml @11:13:10.13 SC.coast @11:13:10.17 collectSample 1500ml w/bubble point of 55psi @11:13:10.19 Priming 7ml @11:13:10.20 Intake.select :close @11:13:10.21 Exhaust.select :close @11:13:10.22 SS.hold @11:13:10.44 Calibrating pressure sensors @11:13:10.46 CTV.dial :air @11:13:11.78 SSV.dial :spit,avoiding: :exhaust @11:13:11.80 SS.reconfigure SSfast @11:13:11.85 SS.seek 5ml @11:13:14.45 SSV.dial CBV @11:13:14.48 CBV.dial SSV @11:13:15.89 delay 3.8 seconds @11:13:19.84 SSV.dial :spit @11:13:21.18 SS.seek 0ml @11:13:23.77 SSV.dial CBV @11:13:25.09 CTV.dial :intake @11:13:26.39 SSV.dial CBV @11:13:26.42 CBV.dial SSV @11:13:26.44 Allowing 40s for 7.0ml of remaining 7.0ml @11:13:26.45 Intake.select :open @11:13:28.63 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:13:28.65 SS.reconfigure SSinhale.with maxSpeed: 150 @11:13:28.70 SS.seek 7ml @11:13:34.08 Stroke completed -- waiting for pressure to equalize ... @11:13:47.42 Intake.select :close @11:13:47.58 SSV.dial :exhaust @11:13:48.35 Exhaust.select :open @11:13:48.47 SS.reconfigure SSexhale @11:13:48.52 SS.seek 0ml @11:13:52.10 Exhaust.select :close @11:13:52.21 Sampling 1500.0ml @11:13:52.23 Intake.select :close @11:13:52.24 Exhaust.select :close @11:13:52.25 SS.reconfigure SSconfig @11:13:52.30 SS.hold @11:13:52.52 CTV.dial :intake @11:13:52.54 SSV.dial CBV @11:13:52.57 CBV.dial SSV @11:13:53.38 Allowing 130s for 25.0ml of remaining 1500.0ml @11:13:53.39 Intake.select :open @11:13:55.58 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:13:55.59 SS.reconfigure SSinhale.with maxSpeed: 150 @11:13:55.64 SS.seek 25ml @11:13:59.51 Vacuum too high -- waiting up to 40s @11:14:02.11 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:14:02.15 SS.reconfigure SSinhale.with maxSpeed: 135 @11:14:02.23 SS.seek 25ml @11:14:05.14 Vacuum too high -- waiting up to 40s @11:14:07.53 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:14:07.55 SS.reconfigure SSinhale.with maxSpeed: 122 @11:14:07.60 SS.seek 25ml @11:14:10.54 Vacuum too high -- waiting up to 40s @11:14:12.68 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:14:12.70 SS.reconfigure SSinhale.with maxSpeed: 110 @11:14:12.75 SS.seek 25ml @11:14:15.22 Vacuum too high -- waiting up to 40s @11:14:17.35 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:14:17.37 SS.reconfigure SSinhale.with maxSpeed: 99 @11:14:17.42 SS.seek 25ml @11:14:19.79 Vacuum too high -- waiting up to 40s @11:14:21.90 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:14:21.92 SS.reconfigure SSinhale.with maxSpeed: 89 @11:14:21.97 SS.seek 25ml @11:14:24.69 Vacuum too high -- waiting up to 40s @11:14:26.79 SS.reconfigure SSinhale.with maxSpeed: 80 @11:14:26.84 SS.seek 25ml @11:14:29.45 Vacuum too high -- waiting up to 40s @11:14:31.34 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:14:31.38 SS.reconfigure SSinhale.with maxSpeed: 72 @11:14:31.45 SS.seek 25ml @11:14:34.45 Vacuum too high -- waiting up to 40s @11:14:36.24 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:14:36.26 SS.reconfigure SSinhale.with maxSpeed: 65 @11:14:36.30 SS.seek 25ml @11:14:39.41 Stroke completed -- waiting for pressure to equalize ... @11:14:43.93 Intake.select :close @11:14:44.09 SSV.dial :exhaust @11:14:44.88 Exhaust.select :open @11:14:45.00 SS.reconfigure SSexhale @11:14:45.05 SS.seek 0ml @11:14:54.13 Exhaust.select :close @11:14:54.25 SSV.dial CBV @11:14:54.27 CBV.dial SSV @11:14:55.07 Allowing 130s for 25.0ml of remaining 1475.0ml @11:14:55.08 Intake.select :open @11:14:57.27 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:14:57.29 SS.reconfigure SSinhale.with maxSpeed: 65 @11:14:57.34 SS.seek 25ml @11:15:02.87 Vacuum too high -- waiting up to 40s @11:15:04.72 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:15:04.74 SS.reconfigure SSinhale.with maxSpeed: 59 @11:15:04.79 SS.seek 25ml @11:15:09.90 Vacuum too high -- waiting up to 40s @11:15:11.49 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:15:11.51 SS.reconfigure SSinhale.with maxSpeed: 53 @11:15:11.56 SS.seek 25ml @11:15:18.07 Vacuum too high -- waiting up to 40s @11:15:19.65 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:15:19.66 SS.reconfigure SSinhale.with maxSpeed: 48 @11:15:19.71 SS.seek 25ml @11:15:27.72 Vacuum too high -- waiting up to 40s @11:15:29.57 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:15:29.59 SS.reconfigure SSinhale.with maxSpeed: 43 @11:15:29.66 SS.seek 25ml @11:15:41.87 Stroke completed -- waiting for pressure to equalize ... @11:15:46.95 Intake.select :close @11:15:47.11 SSV.dial :exhaust @11:15:47.89 Exhaust.select :open @11:15:48.01 SS.reconfigure SSexhale @11:15:48.06 SS.seek 0ml @11:15:57.08 Exhaust.select :close @11:15:57.20 SSV.dial CBV @11:15:57.22 CBV.dial SSV @11:15:58.03 Allowing 130s for 25.0ml of remaining 1450.0ml @11:15:58.04 Intake.select :open @11:16:00.23 Intake @31.1psia, vacuum from 20.0 to 24.0psi @11:16:00.25 SS.reconfigure SSinhale.with maxSpeed: 43 @11:16:00.30 SS.seek 25ml @11:16:22.63 Vacuum too high -- waiting up to 40s @11:16:24.49 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:16:24.51 SS.reconfigure SSinhale.with maxSpeed: 39 @11:16:24.56 SS.seek 25ml @11:16:31.37 delay 5 seconds @11:16:36.40 ATV.dial ASV @11:16:37.35 ASV.dial :SPEdelivery @11:16:37.37 AS.seek 0.02ml @11:16:45.77 Stroke completed -- waiting for pressure to equalize ... @11:16:50.85 Intake.select :close @11:16:51.01 SSV.dial :exhaust @11:16:51.80 Exhaust.select :open @11:16:51.92 SS.reconfigure SSexhale @11:16:51.96 SS.seek 0ml @11:17:00.85 Exhaust.select :close @11:17:00.96 SSV.dial CBV @11:17:00.99 CBV.dial SSV @11:17:01.78 Allowing 130s for 25.0ml of remaining 1425.0ml @11:17:01.79 Intake.select :open @11:17:03.97 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:17:03.99 SS.reconfigure SSinhale.with maxSpeed: 39 @11:17:04.04 SS.seek 25ml @11:17:49.27 Stroke completed -- waiting for pressure to equalize ... @11:17:55.03 Intake.select :close @11:17:55.19 SSV.dial :exhaust @11:17:55.97 Exhaust.select :open @11:17:56.09 SS.reconfigure SSexhale @11:17:56.14 SS.seek 0ml @11:18:05.82 Exhaust.select :close @11:18:05.94 SSV.dial CBV @11:18:05.96 CBV.dial SSV @11:18:06.77 Allowing 130s for 25.0ml of remaining 1400.0ml @11:18:06.78 Intake.select :open @11:18:08.97 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:18:08.99 SS.reconfigure SSinhale.with maxSpeed: 39 @11:18:09.04 SS.seek 25ml @11:18:47.95 Vacuum too high -- waiting up to 40s @11:18:49.83 Intake @30.9psia, vacuum from 20.0 to 24.0psi @11:18:49.85 SS.reconfigure SSinhale.with maxSpeed: 35 @11:18:49.90 SS.seek 25ml @11:18:57.24 Stroke completed -- waiting for pressure to equalize ... @11:19:01.71 Intake.select :close @11:19:01.87 SSV.dial :exhaust @11:19:02.66 Exhaust.select :open @11:19:02.78 SS.reconfigure SSexhale @11:19:02.83 SS.seek 0ml @11:19:11.41 Exhaust.select :close @11:19:11.53 SSV.dial CBV @11:19:11.55 CBV.dial SSV @11:19:12.36 Allowing 130s for 25.0ml of remaining 1375.0ml @11:19:12.37 Intake.select :open @11:19:14.56 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:19:14.58 SS.reconfigure SSinhale.with maxSpeed: 35 @11:19:14.63 SS.seek 25ml @11:19:54.73 delay 5 seconds @11:19:59.76 ATV.dial :air2 @11:20:00.78 AS.seek 0.52ml @11:20:05.14 Stroke completed -- waiting for pressure to equalize ... @11:20:10.23 Intake.select :close @11:20:10.39 SSV.dial :exhaust @11:20:11.18 Exhaust.select :open @11:20:11.30 SS.reconfigure SSexhale @11:20:11.35 SS.seek 0ml @11:20:20.43 Exhaust.select :close @11:20:20.54 SSV.dial CBV @11:20:20.57 CBV.dial SSV @11:20:21.38 Allowing 130s for 25.0ml of remaining 1350.0ml @11:20:21.39 Intake.select :open @11:20:23.57 Intake @31.1psia, vacuum from 20.0 to 24.0psi @11:20:23.59 SS.reconfigure SSinhale.with maxSpeed: 35 @11:20:23.64 SS.seek 25ml @11:21:13.99 Stroke completed -- waiting for pressure to equalize ... @11:21:20.27 Intake.select :close @11:21:20.44 SSV.dial :exhaust @11:21:21.22 Exhaust.select :open @11:21:21.34 SS.reconfigure SSexhale @11:21:21.39 SS.seek 0ml @11:21:30.08 Exhaust.select :close @11:21:30.20 SSV.dial CBV @11:21:30.22 CBV.dial SSV @11:21:31.02 Allowing 130s for 25.0ml of remaining 1325.0ml @11:21:31.04 Intake.select :open @11:21:33.22 Intake @31.1psia, vacuum from 20.0 to 24.0psi @11:21:33.24 SS.reconfigure SSinhale.with maxSpeed: 35 @11:21:33.29 SS.seek 25ml @11:21:41.68 delay 5 seconds @11:21:46.71 ATV.dial ASV @11:21:47.65 ASV.dial :wasteR @11:21:48.02 AS.seek 0.3ml @11:22:23.79 Stroke completed -- waiting for pressure to equalize ... @11:22:30.13 Intake.select :close @11:22:30.29 SSV.dial :exhaust @11:22:31.07 Exhaust.select :open @11:22:31.19 SS.reconfigure SSexhale @11:22:31.23 SS.seek 0ml @11:22:32.56 delay 20 seconds @11:22:40.15 Exhaust.select :close @11:22:40.27 SSV.dial CBV @11:22:40.29 CBV.dial SSV @11:22:41.10 Allowing 130s for 25.0ml of remaining 1300.0ml @11:22:41.11 Intake.select :open @11:22:43.29 Intake @31.0psia, vacuum from 20.0 to 24.0psi @11:22:43.31 SS.reconfigure SSinhale.with maxSpeed: 35 @11:22:43.36 SS.seek 25ml @11:22:52.60 AS.seek 0.07ml @11:23:24.39 Vacuum too high -- waiting up to 40s @11:23:26.48 Intake @30.9psia, vacuum from 20.0 to 24.0psi @11:23:26.49 SS.reconfigure SSinhale.with maxSpeed: 32 @11:23:26.54 SS.seek 25ml @11:23:36.82 Stroke completed -- waiting for pressure to equalize ... @11:23:39.12 delay 20 seconds @11:23:42.54 Intake.select :close @11:23:42.70 SSV.dial :exhaust @11:23:44.02 Exhaust.select :open @11:23:44.17 SS.reconfigure SSexhale @11:23:44.22 SS.seek 0ml @11:23:53.21 Exhaust.select :close @11:23:53.33 SSV.dial CBV @11:23:53.35 CBV.dial SSV @11:23:54.15 Allowing 130s for 25.0ml of remaining 1275.0ml @11:23:54.16 Intake.select :open @11:23:56.34 Intake @31.1psia, vacuum from 20.0 to 24.0psi @11:23:56.36 SS.reconfigure SSinhale.with maxSpeed: 32 @11:23:56.41 SS.seek 25ml @11:23:59.14 ATV.dial :waste2 @11:23:59.87 AS.reconfigure ASconfig @11:23:59.93 AS.seek :empty @11:24:04.30 Elution @11:24:04.31 ATV.dial :air1 @11:24:05.40 AS.reconfigure ASslow1 @11:24:05.45 AS.seek 0.049ml @11:24:15.83 delay 3 seconds @11:24:18.86 ATV.dial :ElutionWater @11:24:19.52 AS.seek 0.109ml @11:24:31.84 delay 3 seconds @11:24:34.87 ATV.dial ASV,via: :air2 @11:24:36.85 ASV.dial :SPEelution @11:24:37.42 AFV.select :carrierSolution @11:24:37.58 AS.reconfigure ASnorm @11:24:37.64 AS.seek 0.759ml @11:24:51.41 Stroke completed -- waiting for pressure to equalize ... @11:24:58.40 Intake.select :close @11:24:58.56 SSV.dial :exhaust @11:24:59.35 Exhaust.select :open @11:24:59.47 SS.reconfigure SSexhale @11:24:59.52 SS.seek 0ml @11:25:08.19 Exhaust.select :close @11:25:08.31 SSV.dial CBV @11:25:08.33 CBV.dial SSV @11:25:09.13 Allowing 130s for 25.0ml of remaining 1250.0ml @11:25:09.14 Intake.select :open @11:25:10.72 delay 3 seconds @11:25:11.33 Intake @31.1psia, vacuum from 20.0 to 24.0psi @11:25:11.35 SS.reconfigure SSinhale.with maxSpeed: 32 @11:25:11.40 SS.seek 25ml @11:25:13.75 AFV.select :holdingCoil @11:25:13.90 AS.reconfigure ASslow1 @11:25:13.96 AS.seek 0.474ml @11:26:01.23 Vacuum too high -- waiting up to 40s @11:26:03.83 Intake @30.9psia, vacuum from 20.0 to 24.0psi @11:26:03.85 SS.reconfigure SSinhale.with maxSpeed: 29 @11:26:03.90 SS.seek 25ml @11:26:09.93 Stroke completed -- waiting for pressure to equalize ... @11:26:11.52 delay 5 seconds @11:26:15.65 Intake.select :close @11:26:15.82 SSV.dial :exhaust @11:26:16.60 AS.reconfigure ASslow2 @11:26:16.67 AS.seek 0.439ml @11:26:16.69 Exhaust.select :open @11:26:16.84 SS.reconfigure SSexhale @11:26:16.89 SS.seek 0ml @11:26:25.57 Exhaust.select :close @11:26:25.69 SSV.dial CBV @11:26:25.71 CBV.dial SSV @11:26:26.52 Allowing 130s for 25.0ml of remaining 1225.0ml @11:26:26.53 Intake.select :open @11:26:28.71 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:26:28.73 SS.reconfigure SSinhale.with maxSpeed: 29 @11:26:28.78 SS.seek 25ml @11:26:51.98 delay 1:00 @11:27:29.44 Stroke completed -- waiting for pressure to equalize ... @11:27:37.09 Intake.select :close @11:27:37.25 SSV.dial :exhaust @11:27:38.02 Exhaust.select :open @11:27:38.14 SS.reconfigure SSexhale @11:27:38.18 SS.seek 0ml @11:27:46.88 Exhaust.select :close @11:27:46.99 SSV.dial CBV @11:27:47.02 CBV.dial SSV @11:27:47.82 Allowing 130s for 25.0ml of remaining 1200.0ml @11:27:47.83 Intake.select :open @11:27:50.01 Intake @31.1psia, vacuum from 20.0 to 24.0psi @11:27:50.03 SS.reconfigure SSinhale.with maxSpeed: 29 @11:27:50.08 SS.seek 25ml @11:27:52.01 pushing eluate to holding coil @11:27:52.09 AS.seek 0.279ml @11:28:32.89 Vacuum too high -- waiting up to 40s @11:28:35.49 Intake @31.0psia, vacuum from 20.0 to 24.0psi @11:28:35.51 SS.reconfigure SSinhale.with maxSpeed: 26 @11:28:35.56 SS.seek 25ml @11:28:55.74 Stroke completed -- waiting for pressure to equalize ... @11:29:03.32 Intake.select :close @11:29:03.48 SSV.dial :exhaust @11:29:04.26 Exhaust.select :open @11:29:04.38 SS.reconfigure SSexhale @11:29:04.43 SS.seek 0ml @11:29:13.38 Exhaust.select :close @11:29:13.50 SSV.dial CBV @11:29:13.52 CBV.dial SSV @11:29:14.32 Allowing 130s for 25.0ml of remaining 1175.0ml @11:29:14.33 Intake.select :open @11:29:16.52 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:29:16.54 SS.reconfigure SSinhale.with maxSpeed: 26 @11:29:16.59 SS.seek 25ml @11:30:23.76 Stroke completed -- waiting for pressure to equalize ... @11:30:33.21 delay 30 seconds @11:30:33.98 Intake.select :close @11:30:34.14 SSV.dial :exhaust @11:30:34.93 Exhaust.select :open @11:30:35.05 SS.reconfigure SSexhale @11:30:35.10 SS.seek 0ml @11:30:44.07 Exhaust.select :close @11:30:44.19 SSV.dial CBV @11:30:44.21 CBV.dial SSV @11:30:44.99 Allowing 130s for 25.0ml of remaining 1150.0ml @11:30:45.00 Intake.select :open @11:30:47.18 Intake @31.1psia, vacuum from 20.0 to 24.0psi @11:30:47.20 SS.reconfigure SSinhale.with maxSpeed: 26 @11:30:47.25 SS.seek 25ml @11:31:03.25 ATV.dial :waste,via: :air3 @11:31:04.86 AS.reconfigure ASconfig @11:31:04.91 AS.seek :empty @11:31:21.30 Parking Bleach in LysateSource line and SPEcolumn @11:31:21.31 ATV.dial :air3 @11:31:22.12 AS.reconfigure ASslow1 @11:31:22.17 AS.seek 0.029ml @11:31:28.53 ATV.dial :ElutionWater @11:31:29.12 AS.seek 0.059ml @11:31:32.79 Vacuum too high -- waiting up to 40s @11:31:35.38 ATV.dial :air3 @11:31:36.32 Intake @31.0psia, vacuum from 20.0 to 24.0psi @11:31:36.35 AS.seek 0.069ml @11:31:36.37 SS.reconfigure SSinhale.with maxSpeed: 23 @11:31:36.49 SS.seek 25ml @11:31:38.73 delay 2 seconds @11:31:40.76 ATV.dial :bleach @11:31:41.23 AS.seek 0.769ml @11:32:01.91 Stroke completed -- waiting for pressure to equalize ... @11:32:10.81 Intake.select :close @11:32:10.97 SSV.dial :exhaust @11:32:11.76 Exhaust.select :open @11:32:11.88 SS.reconfigure SSexhale @11:32:11.93 SS.seek 0ml @11:32:20.68 Exhaust.select :close @11:32:20.80 SSV.dial CBV @11:32:20.82 CBV.dial SSV @11:32:21.62 Allowing 130s for 25.0ml of remaining 1125.0ml @11:32:21.63 Intake.select :open @11:32:23.82 Intake @31.1psia, vacuum from 20.0 to 24.0psi @11:32:23.84 SS.reconfigure SSinhale.with maxSpeed: 23 @11:32:23.88 SS.seek 25ml @11:33:28.20 Vacuum too high -- waiting up to 40s @11:33:31.79 Intake @30.9psia, vacuum from 20.0 to 24.0psi @11:33:31.81 SS.reconfigure SSinhale.with maxSpeed: 21 @11:33:31.86 SS.seek 25ml @11:33:45.23 Stroke completed -- waiting for pressure to equalize ... @11:33:55.33 Intake.select :close @11:33:55.49 SSV.dial :exhaust @11:33:56.27 Exhaust.select :open @11:33:56.39 SS.reconfigure SSexhale @11:33:56.44 SS.seek 0ml @11:34:02.27 delay 3 seconds @11:34:05.24 Exhaust.select :close @11:34:05.29 ATV.dial :air3 @11:34:05.36 SSV.dial CBV @11:34:05.38 CBV.dial SSV @11:34:05.84 delay 3 seconds @11:34:06.18 Allowing 130s for 25.0ml of remaining 1100.0ml @11:34:06.20 Intake.select :open @11:34:08.37 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:34:08.39 SS.reconfigure SSinhale.with maxSpeed: 21 @11:34:08.44 SS.seek 25ml @11:34:08.93 AS.seek 0.794ml @11:34:14.22 delay 3 seconds @11:34:17.25 ATV.dial :bleach @11:34:17.86 AS.seek 0.779ml @11:34:21.16 delay 3 seconds @11:34:24.18 ATV.dial :AIV @11:34:25.38 AS.reconfigure ASconfig @11:34:25.44 AS.seek 0.015ml @11:35:09.65 ATV.dial :waste @11:35:10.72 Vacuum too high -- waiting up to 40s @11:35:11.05 AS.seek :empty @11:35:12.31 ATV.dial :air3 @11:35:12.97 AS.reconfigure ASslow1 @11:35:13.01 AS.seek 0.009ml @11:35:14.27 Intake @30.9psia, vacuum from 20.0 to 24.0psi @11:35:14.29 SS.reconfigure SSinhale.with maxSpeed: 19 @11:35:14.34 SS.seek 25ml @11:35:15.34 delay 2 seconds @11:35:17.36 ATV.dial :bleach @11:35:17.83 AS.seek 0.579ml @11:35:38.12 Stroke completed -- waiting for pressure to equalize ... @11:35:49.42 Intake.select :close @11:35:49.58 SSV.dial :exhaust @11:35:50.37 Exhaust.select :open @11:35:50.49 SS.reconfigure SSexhale @11:35:50.54 SS.seek 0ml @11:35:59.40 Exhaust.select :close @11:35:59.52 SSV.dial CBV @11:35:59.54 CBV.dial SSV @11:36:00.35 Allowing 130s for 25.0ml of remaining 1075.0ml @11:36:00.36 Intake.select :open @11:36:02.55 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:36:02.57 SS.reconfigure SSinhale.with maxSpeed: 19 @11:36:02.62 SS.seek 25ml @11:37:12.71 delay 3 seconds @11:37:15.20 Vacuum too high -- waiting up to 40s @11:37:15.74 ATV.dial :air3 @11:37:16.29 delay 3 seconds @11:37:19.38 AS.seek 0.604ml @11:37:19.89 Intake @31.0psia, vacuum from 20.0 to 24.0psi @11:37:19.91 SS.reconfigure SSinhale.with maxSpeed: 17 @11:37:19.95 SS.seek 25ml @11:37:24.71 delay 3 seconds @11:37:27.74 ATV.dial :bleach @11:37:28.34 AS.seek 0.589ml @11:37:31.62 delay 3 seconds @11:37:34.64 ATV.dial :ASV @11:37:35.63 ASV.dial :wasteR @11:37:36.20 AS.seek 0ml @11:37:42.35 Stroke completed -- waiting for pressure to equalize ... @11:37:55.72 Intake.select :close @11:37:55.89 SSV.dial :exhaust @11:37:56.68 Exhaust.select :open @11:37:56.80 SS.reconfigure SSexhale @11:37:56.85 SS.seek 0ml @11:38:05.79 Exhaust.select :close @11:38:05.91 SSV.dial CBV @11:38:05.93 CBV.dial SSV @11:38:06.74 Allowing 130s for 25.0ml of remaining 1050.0ml @11:38:06.75 Intake.select :open @11:38:08.94 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:38:08.96 SS.reconfigure SSinhale.with maxSpeed: 17 @11:38:09.01 SS.seek 25ml @11:39:18.05 Vacuum too high -- waiting up to 40s @11:39:22.45 Intake @31.0psia, vacuum from 20.0 to 24.0psi @11:39:22.47 SS.reconfigure SSinhale.with maxSpeed: 15 @11:39:22.52 SS.seek 25ml @11:39:34.95 delay 1:00 @11:40:01.95 Stroke completed -- waiting for pressure to equalize ... @11:40:16.77 SS.reconfigure SSconfig @11:40:16.82 SS.hold @11:40:16.96 Intake.select :close @11:40:17.13 ShallowSampler::Clogged in WCR -- progress too slow after 475.0ml @11:40:17.14 SSV.dial :exhaust @11:40:17.93 Exhaust.select :open @11:40:18.63 SS.reconfigure SSexhale @11:40:18.68 SS.seek 0ml @11:40:27.89 Exhaust.select :close @11:40:28.00 Sampled 475.0ml @11:40:28.02 Drying puck before fixing for 4:00 @11:40:28.03 CBV.dial :waste2b @11:40:28.81 delay 1:00 @11:40:34.98 AFV.select :carrierSolution @11:40:35.08 delay 2 seconds @11:40:37.11 AS.reconfigure ASconfig @11:40:37.16 AS.seek 1ml @11:41:28.84 CSV.dial :air @11:41:29.31 CS.reconfigure CSfast @11:41:29.36 CS.seek 10ml @11:41:34.90 delay 1 second @11:41:35.92 AFV.select :holdingCoil @11:41:36.02 delay 1 second @11:41:37.05 ATV.dial ASV @11:41:37.06 ASV.dial :wasteR @11:41:37.08 AS.reconfigure ASslow1 @11:41:37.12 AS.seek 0ml @11:41:51.95 Pumping wcr puck from tube 2 up to 55psi... @11:41:51.97 CBV.dial SSV @11:41:53.30 CBV.dial between CSV and waste2b @11:41:54.29 CSV.dial between air and CTV @11:41:54.65 CS.seek 7.132ml @11:42:01.87 CTV.dial CSV,avoiding: :air @11:42:01.89 CSV.dial CTV @11:42:02.73 CS.reconfigure CSpressurize @11:42:02.78 CS.seek 0ml @11:42:20.10 CBV.dial :waste2b @11:42:20.45 CS.reconfigure CSconfig @11:42:20.50 CS.seek :empty @11:42:22.89 delay 1:00 @11:43:22.92 CTV.dial :air,avoiding: :intake @11:43:24.24 Fix #1 of 2 (with rnal) for 2:00 @11:43:24.26 CSV.dial CSR @11:43:24.77 Pulling 2ml of CSR.rnal @11:43:24.79 CSR.select :rnal @11:43:24.91 CS.seek 2ml @11:43:30.99 delay 5 seconds @11:43:36.02 CSR.select :air @11:43:36.15 CS.reconfigure CSfast @11:43:36.20 CS.seek 10ml @11:43:54.24 Pumping wcr puck from tube 2 up to 55psi... @11:43:54.26 CBV.dial SSV @11:43:55.59 CBV.dial between CSV and waste2b @11:43:56.57 CSV.dial between air and CTV @11:43:57.31 CS.seek 7.157ml @11:44:04.44 CTV.dial CSV,avoiding: :air @11:44:04.46 CSV.dial CTV @11:44:05.84 CS.reconfigure CSpressurize @11:44:05.89 CS.seek 0ml @11:44:24.23 delay 2:00 @11:44:58.62 delay 20 seconds @11:45:18.65 ATV.dial :air3 @11:45:20.08 AS.seek 0.59ml @11:46:24.26 CBV.dial :waste2b @11:46:24.62 CS.reconfigure CSconfig @11:46:24.67 CS.seek :empty @11:46:26.05 delay 1:00 @11:47:19.00 delay 3 seconds @11:47:22.03 ATV.dial :ASV @11:47:23.37 ASV.dial :wasteR @11:47:23.45 AS.seek 0.09ml @11:47:26.08 CTV.dial :air,avoiding: :intake @11:47:27.40 Fix #2 of 2 (with rnal) for 2:00 @11:47:27.95 CSV.dial CSR @11:47:28.47 Pulling 2ml of CSR.rnal @11:47:28.49 CSR.select :rnal @11:47:28.61 CS.seek 2ml @11:47:34.80 delay 5 seconds @11:47:39.83 CSR.select :air @11:47:39.96 CS.reconfigure CSfast @11:47:40.01 CS.seek 10ml @11:47:58.12 Pumping wcr puck from tube 2 up to 55psi... @11:47:58.14 CBV.dial SSV @11:47:59.48 CBV.dial between CSV and waste2b @11:48:00.47 CSV.dial between air and CTV @11:48:01.11 CS.seek 7.08ml @11:48:08.47 CTV.dial CSV,avoiding: :air @11:48:08.49 CSV.dial CTV @11:48:09.89 CS.reconfigure CSpressurize @11:48:09.94 CS.seek 0ml @11:48:27.76 delay 2:00 @11:49:04.28 delay 30 seconds @11:49:34.31 ATV.dial :extSrc @11:49:35.05 delay 3 seconds @11:49:38.08 ATV.dial :ElutionWater @11:49:39.16 AS.seek 0.12ml @11:49:45.47 ATV.dial :air3 @11:49:46.08 AS.seek 0.13ml @11:49:48.37 delay 2 seconds @11:49:50.39 ATV.dial :bleach @11:49:50.84 AS.seek 0.23ml @11:50:11.22 delay 3 seconds @11:50:14.25 ATV.dial :air3 @11:50:14.78 delay 3 seconds @11:50:17.87 AS.seek 0.255ml @11:50:23.16 delay 3 seconds @11:50:26.19 ATV.dial :bleach @11:50:26.82 AS.seek 0.24ml @11:50:27.79 CBV.dial :waste2b @11:50:28.14 CS.reconfigure CSconfig @11:50:28.20 CS.seek :empty @11:50:29.87 delay 1:00 @11:50:30.14 delay 3 seconds @11:50:33.16 ATV.dial :ASV @11:50:34.16 ASV.dial :SPEdelivery @11:50:34.55 AFV.select :carrierSolution @11:50:34.66 delay 2 seconds @11:50:36.69 AS.reconfigure ASconfig @11:50:36.74 AS.seek 0.5ml @11:50:51.97 delay 1 second @11:50:52.99 AFV.select :holdingCoil @11:50:53.14 AS.reconfigure ASslow1 @11:50:53.19 AS.seek 0.17ml @11:51:29.90 CTV.dial :air,avoiding: :intake @11:51:31.29 Storing wcr puck in Collection Clamp to tube 1 @11:51:31.48 SC.reconfigure SCtight @11:51:31.54 SC.seek 1 @11:51:33.68 SC.reconfigure SCloose @11:51:33.91 Elbow.reconfigure TightElbow @11:51:33.96 Elbow.seek 1 @11:51:35.83 Forearm.reconfigure TightForearm @11:51:35.89 Forearm.seek 1 + 700 counts @11:51:36.93 SE.reconfigure SEpick @11:51:37.01 SE.seek beamBreak @11:51:39.44 SE.configure SEconfig @11:51:40.16 Elbow.reconfigure LimpElbow @11:51:40.21 Forearm.reconfigure LooseForearm @11:51:40.26 Forearm.seek 1 @11:51:40.53 Elbow.hold @11:51:40.61 Elbow.reconfigure TightElbow @11:51:40.74 SE.seek 7.08 Pucks below Top Plate @11:51:41.94 Forearm.force -20 @11:51:41.98 Forearm.seek 1 + 345 counts @11:51:42.29 Forearm.reconfigure TightForearm @11:51:42.35 Forearm.seek 1 + 700 counts @11:51:42.60 SE.seek 8.08 Pucks below Top Plate @11:51:43.33 Forearm.reconfigure LooseForearm @11:51:43.39 Forearm.seek :retract @11:51:44.31 Elbow.seek CC @11:51:45.99 Elbow.reconfigure LooseElbow @11:51:46.10 Forearm.seek CC @11:51:47.14 Hand.close @11:51:47.70 CC.seek :open @11:51:52.20 Forearm.seek :retract @11:51:53.20 Elbow.reconfigure TightElbow @11:51:53.25 Elbow.seek 1 @11:51:55.18 Elbow.reconfigure LimpElbow @11:51:55.24 Forearm.seek 1 @11:51:56.23 Elbow.hold @11:51:56.31 Elbow.reconfigure TightElbow @11:51:56.36 Hand.open @11:51:57.09 Forearm.force -20 @11:51:57.13 Forearm.seek 1 + 349 counts @11:51:57.45 Forearm.reconfigure TightForearm @11:51:57.50 Forearm.seek 1 + 700 counts @11:51:57.84 SE.seek :down @11:51:58.99 Forearm.reconfigure LooseForearm @11:51:59.04 Forearm.seek :retract @11:51:59.86 SPE.hold 2:46:40,95C @11:51:59.92 delay 3:00 @11:52:00.00 SE.coast @11:52:00.05 SC.coast @11:52:00.09 Starting finalPurge @11:52:00.11 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:52:00.18 Hand.open @11:52:00.59 Elbow.seek FlushPuck::Garage @11:52:02.63 Elbow.reconfigure LooseElbow @11:52:02.74 Forearm.seek :garage @11:52:03.75 Hand.close @11:52:05.03 Forearm.seek :retract @11:52:06.03 Elbow.reconfigure TightElbow @11:52:06.09 Elbow.seek CC @11:52:09.01 Elbow.reconfigure LooseElbow @11:52:09.11 Forearm.reconfigure TightForearm @11:52:09.16 Forearm.seek CC @11:52:10.48 CC.seek :closed @11:52:14.75 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.89 @11:52:14.83 Hand.open @11:52:15.52 Forearm.reconfigure LooseForearm @11:52:15.57 Forearm.seek :retract @11:52:16.59 Flushing the Collection Valve::Manifold @11:52:16.73 CSV.dial CSR @11:52:17.25 Pulling 5ml of CSR.flush @11:52:17.26 CSR.select :flush @11:52:17.39 CS.seek 5ml @11:52:31.15 delay 5 seconds @11:52:36.18 CSR.select :air @11:52:36.36 CS.reconfigure CSfast @11:52:36.41 CS.seek 7.5ml @11:52:43.09 CSV.dial :waste @11:52:44.42 CS.reconfigure CSconfig @11:52:44.47 CS.seek 0ml @11:53:04.66 Flushing lower manifold's waste1 port @11:53:04.80 CSV.dial :flush @11:53:05.28 CS.reconfigure CSfast @11:53:05.33 CS.seek 5ml @11:53:17.44 delay 5 seconds @11:53:22.47 CSV.dial :air @11:53:23.01 CS.seek 7ml @11:53:28.53 CSV.dial CTV @11:53:28.57
CTV.dial CSV @11:53:29.98 CBV.dial :waste1 @11:53:30.79 CS.seek 0ml @11:53:47.31 Flushing lower manifold's waste2b port @11:53:47.45 CSV.dial :flush @11:53:48.34 CS.seek 5ml @11:54:00.41 delay 5 seconds @11:54:05.44 CSV.dial :air @11:54:05.98 CS.seek 7ml @11:54:11.50 CSV.dial CTV @11:54:11.53
CTV.dial CSV @11:54:12.00 CBV.dial :waste2b @11:54:12.82 CS.seek 0ml @11:54:29.34 Flushing upper manifold @11:54:29.48 CSV.dial :flush @11:54:30.24 CS.seek 5ml @11:54:42.22 delay 5 seconds @11:54:47.25 CSV.dial :air @11:54:47.79 CS.seek 7ml @11:54:53.25 CSV.dial CBV @11:54:53.29
CBV.dial CSV @11:54:54.39 CTV.dial :air @11:54:55.75 CS.seek 0ml @11:54:59.97 AFV.select :carrierSolution @11:55:00.07 delay 2 seconds @11:55:02.10 AS.reconfigure ASconfig @11:55:02.15 AS.seek 1ml @11:55:12.69
delay 7 seconds @11:55:19.73 CS.seek 3ml @11:55:27.31 CSV.dial :waste @11:55:27.78 CS.seek 0ml @11:55:35.57 CSV.dial :flush @11:55:36.04 CS.seek 3ml @11:55:43.63 delay 5 seconds @11:55:48.66 CSV.dial :air @11:55:49.20 CS.seek 5ml @11:55:50.21 delay 1 second @11:55:51.23 AFV.select :holdingCoil @11:55:51.33 delay 1 second @11:55:52.35 ASV.dial :wasteD @11:55:53.32 AS.seek 0ml @11:55:54.75
CSV.dial :air @11:55:54.77 CS.seek 0ml @11:56:06.85 delay 7 seconds @11:56:13.89 CS.seek 3ml @11:56:21.41 CSV.dial :waste @11:56:22.18 CS.seek 0ml @11:56:29.82 Disinfecting external Intake with mfbkill @11:56:29.96 CSV.dial CSR @11:56:31.33 Pulling 8ml of CSR.mfbkill @11:56:31.34 CSR.select :mfbkill @11:56:31.47 CS.seek 8ml @11:56:49.72 delay 5 seconds @11:56:51.12 Priming Eluate @11:56:51.13 AFV.select :carrierSolution @11:56:51.24 delay 2 seconds @11:56:53.28 AS.seek 0.5ml @11:56:54.74
CSR.select :air @11:56:54.86 CS.seek 10ml @11:57:00.25 CSV.dial CBV @11:57:00.28
CBV.dial CSV @11:57:01.32 CTV.dial :intake @11:57:02.08 Intake.select :open @11:57:02.20 CS.seek 2ml @11:57:21.44 Intake.select :close @11:57:21.55 CTV.dial :air @11:57:22.28 delay 1 second @11:57:22.33
CS.reconfigure CSconfig @11:57:22.38 CS.seek 0ml @11:57:23.31 AFV.select :holdingCoil @11:57:23.41 ATV.dial ASV @11:57:23.42 ASV.dial :SPErecovery @11:57:23.99 Swish #1 of 2 @11:57:24.07 AS.reconfigure ASslow0 @11:57:24.12 AS.seek 0.6ml @11:57:28.66
Flushing lower manifold's waste1 port @11:57:28.67 CSV.dial CSR @11:57:29.77 Pulling 4ml of CSR.mfbkill @11:57:29.78 CSR.select :mfbkill @11:57:29.91 CS.seek 4ml @11:57:34.57 AS.seek 0.5ml @11:57:41.19
delay 5 seconds @11:57:44.92 Swish #2 of 2 @11:57:45.01 AS.seek 0.6ml @11:57:46.21
CSR.select :air @11:57:46.34 CS.reconfigure CSfast @11:57:46.38 CS.seek 10ml @11:57:55.46 AS.seek 0.5ml @11:58:00.35 CSV.dial CTV @11:58:00.39
CTV.dial CSV @11:58:01.80 CBV.dial :waste1 @11:58:02.28 CS.seek 0ml @11:58:05.85 delay 3 seconds @11:58:08.94 AS.reconfigure ASslow1 @11:58:08.99 AS.seek 0.684ml @11:58:24.72
Flushing lower manifold's waste2b port @11:58:24.73 CSV.dial CSR @11:58:25.25 Pulling 4ml of CSR.mfbkill @11:58:25.26 CSR.select :mfbkill @11:58:25.39 CS.reconfigure CSconfig @11:58:25.44 CS.seek 4ml @11:58:36.69 delay 5 seconds @11:58:41.72 CSR.select :air @11:58:41.85 CS.reconfigure CSfast @11:58:41.89 CS.seek 10ml @11:58:46.27 delay 3 seconds @11:58:49.30 Eluate primed @11:58:49.31 ATV.dial :waste2 @11:58:49.94 AS.reconfigure ASnorm @11:58:49.99 AS.seek 0ml @11:58:55.94 CSV.dial CTV @11:58:55.97
CTV.dial CSV @11:58:56.44 CBV.dial :waste2b @11:58:57.25 CS.seek 0ml @11:59:19.79 Killing upper manifold @11:59:19.80 CSV.dial CSR @11:59:20.33 Pulling 4ml of CSR.mfbkill @11:59:20.34 CSR.select :mfbkill @11:59:20.47 CS.reconfigure CSconfig @11:59:20.52 CS.seek 4ml @11:59:24.86 SPE.coast @11:59:24.95 ASV.dial :SPErecovery @11:59:24.97 Setting up PCR for OM -- writing /var/log/esp/OM20may1511h275ml.pcr @11:59:24.99 Building PCR Slug from ASV with MM3 mix, PP5 primer @11:59:25.00 Priming with carrier @11:59:25.01 ATV.dial ARV @11:59:25.96 ARV.dial :carrier @11:59:26.31 AS.seek 0.5ml @11:59:31.69
delay 5 seconds @11:59:36.72 CSR.select :air @11:59:36.85 CS.reconfigure CSfast @11:59:36.89 CS.seek 10ml @11:59:50.63 CSV.dial CBV @11:59:50.66
CBV.dial CSV @11:59:51.74 CTV.dial :air @11:59:51.89 AFV.select :carrierSolution @11:59:52.01 delay 2 seconds @11:59:53.05
CS.seek 0ml @11:59:54.04 AS.reconfigure ASconfig @11:59:54.10 AS.seek 1ml @12:00:15.16
delay 7 seconds @12:00:22.20 CS.seek 3ml @12:00:23.11 delay 1 second @12:00:24.13 AFV.select :holdingCoil @12:00:24.23 delay 1 second @12:00:25.25 ATV.dial :waste @12:00:26.66 AS.reconfigure ASnorm @12:00:26.71 AS.seek :empty @12:00:29.73
CSV.dial :waste @12:00:30.31 CS.seek 0ml @12:00:37.91 Flushing upper manifold @12:00:38.05 CSV.dial :flush @12:00:38.53 CS.seek 5ml @12:00:50.10 delay 5 seconds @12:00:55.13 CSV.dial :air @12:00:55.66 CS.seek 7ml @12:01:00.98 CSV.dial CBV @12:01:01.02
CBV.dial CSV @12:01:02.02 CTV.dial :air @12:01:02.04 CS.seek 0ml @12:01:17.46 AFV.select :carrierSolution @12:01:17.57 delay 2 seconds @12:01:18.07
delay 7 seconds @12:01:19.60 AS.reconfigure ASconfig @12:01:19.65 AS.seek 0.5ml @12:01:25.10
CS.seek 3ml @12:01:32.48 CSV.dial :waste @12:01:32.96 CS.seek 0ml @12:01:40.57 CSV.dial :flush @12:01:41.03 CS.seek 3ml @12:01:48.54 delay 5 seconds @12:01:48.72 delay 1 second @12:01:49.74 AFV.select :holdingCoil @12:01:49.84 Building Slug @12:01:49.85 ATV.dial :ARV @12:01:51.41 delay 2 seconds @12:01:53.44 ARV.dial :air1 @12:01:53.56
CSV.dial :air @12:01:53.82 delay 2 seconds @12:01:54.10
CS.seek 5ml @12:01:55.91 AS.reconfigure ASslow2 @12:01:55.97 AS.seek 0.506ml @12:01:59.45
CSV.dial :air @12:01:59.47 CS.seek 0ml @12:02:02.38 delay 2 seconds @12:02:04.40 ARV.dial :MM3 @12:02:05.42 delay 2 seconds @12:02:07.45 Adding MM3 @12:02:07.52 AS.seek 0.524ml @12:02:11.25
delay 7 seconds @12:02:18.28 CS.seek 3ml @12:02:25.83 CSV.dial :waste @12:02:25.90 delay 25 seconds @12:02:26.58
CS.seek 0ml @12:02:34.07 Flushing lower manifold's waste1 port @12:02:34.21 CSV.dial :flush @12:02:34.68 CS.seek 5ml @12:02:46.35 delay 5 seconds @12:02:50.93 ARV.dial :PP5 @12:02:51.33 Adding PP5 @12:02:51.35 delay 2 seconds @12:02:51.37
CSV.dial :air @12:02:51.89 CS.seek 7ml @12:02:53.43 AS.seek 0.53ml @12:02:57.20 CSV.dial CTV @12:02:57.23
CTV.dial CSV @12:02:58.62 CBV.dial :waste1 @12:02:59.12 CS.seek 0ml @12:02:59.60 delay 10 seconds @12:03:09.63 ARV.dial :air3 @12:03:09.99 adding ARV air @12:03:10.01 delay 2 seconds @12:03:12.09 AS.seek 0.536ml @12:03:15.18
Flushing lower manifold's waste2b port @12:03:15.32 CSV.dial :flush @12:03:16.07 CS.seek 5ml @12:03:18.44 delay 5 seconds @12:03:23.47 ARV.dial :carrier @12:03:24.66 delay 7 seconds @12:03:27.64
delay 5 seconds @12:03:31.76 AS.seek 0.594ml @12:03:32.66
CSV.dial :air @12:03:33.21 CS.seek 7ml @12:03:38.57 CSV.dial CTV @12:03:38.60
CTV.dial CSV @12:03:39.06 CBV.dial :waste2b @12:03:39.87 CS.seek 0ml @12:03:55.70 Disinfecting external Intake with kill @12:03:55.84 CSV.dial CSR @12:03:56.35 Pulling 8ml of CSR.kill @12:03:56.36 CSR.select :kill @12:03:56.49 CS.seek 8ml @12:04:14.45 delay 5 seconds @12:04:19.48 CSR.select :air @12:04:19.61 CS.seek 10ml @12:04:24.99 CSV.dial CBV @12:04:25.03
CBV.dial CSV @12:04:26.11 CTV.dial :intake @12:04:26.86 Intake.select :open @12:04:26.98 CS.seek 2ml @12:04:30.65 delay 5 seconds @12:04:35.68 ATV.dial :ASV @12:04:36.07 delay 2 seconds @12:04:38.16 AS.seek 0.6ml @12:04:44.62 delay 4 seconds @12:04:45.92
Intake.select :close @12:04:46.03 CTV.dial :air @12:04:46.79 CS.reconfigure CSconfig @12:04:46.84 CS.seek 0ml @12:04:48.64 ATV.dial ARV @12:04:49.18 delay 2 seconds @12:04:51.21 ARV.dial :carrier @12:04:51.22 delay 2 seconds @12:04:53.11
CSV.dial CSR @12:04:53.30 AS.seek 0.65ml @12:04:54.18
Pulling 5ml of CSR.flush @12:04:54.19 CSR.select :flush @12:04:54.32 CS.seek 5ml @12:05:08.14 delay 5 seconds @12:05:13.17 CSR.select :air @12:05:13.30 CS.seek 10ml @12:05:27.01 CSV.dial CBV @12:05:28.02 CS.seek 0ml @12:05:43.76 delay 4 seconds @12:05:47.79 ATV.dial PCR @12:05:48.97 ASV.dial :waste1 @12:05:49.73 ARV.dial :air1 @12:05:50.12 Positioning Slug by 0.324ml @12:05:50.21 AS.seek 0.326ml @12:05:54.73
Garaging FlushPuck in Collection Clamp @12:05:54.81 Elbow.reconfigure TightElbow @12:05:54.86 Elbow.seek CC @12:05:55.12 Elbow.reconfigure LooseElbow @12:05:55.22 Forearm.seek CC @12:05:56.23 Hand.close @12:05:56.86 CC.seek :open @12:06:01.24 Forearm.seek :retract @12:06:02.27 Elbow.reconfigure TightElbow @12:06:02.32 Elbow.seek :garage @12:06:05.61 Elbow.reconfigure LooseElbow @12:06:05.71 Forearm.reconfigure TightForearm @12:06:05.76 Forearm.seek :garage @12:06:06.90 Hand.open @12:06:07.55 Forearm.reconfigure LooseForearm @12:06:07.60 Forearm.seek :retract @12:06:08.59 Starting initialPurge @12:06:08.60 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:06:08.68 Hand.open @12:06:09.05 Elbow.reconfigure TightElbow @12:06:09.10 Elbow.seek FlushPuck::Garage @12:06:09.29 Elbow.reconfigure LooseElbow @12:06:09.40 Forearm.seek :garage @12:06:10.37 Hand.close @12:06:11.11 Forearm.seek :retract @12:06:12.10 Elbow.reconfigure TightElbow @12:06:12.15 Elbow.seek CC @12:06:15.13 Elbow.reconfigure LooseElbow @12:06:15.24 Forearm.reconfigure TightForearm @12:06:15.29 Forearm.seek CC @12:06:16.64 CC.seek :closed @12:06:20.92 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.81 @12:06:20.99 Hand.open @12:06:21.67 Forearm.reconfigure LooseForearm @12:06:21.72 Forearm.seek :retract @12:06:22.73 Flushing the Collection Valve::Manifold @12:06:22.86 CSV.dial CSR @12:06:23.93 Pulling 5ml of CSR.flush @12:06:23.94 CSR.select :flush @12:06:24.07 CS.seek 5ml @12:06:37.77 delay 5 seconds @12:06:42.80 CSR.select :air @12:06:42.96 CS.reconfigure CSfast @12:06:43.01 CS.seek 7.5ml @12:06:49.42 CSV.dial :waste @12:06:50.69 CS.reconfigure CSconfig @12:06:50.74 CS.seek 0ml @12:07:10.89 Purging external Intake @12:07:11.02 CSV.dial :flush @12:07:11.49 CS.reconfigure CSfast @12:07:12.14 CS.seek 10ml @12:07:34.20 delay 5 seconds @12:07:39.23 CSV.dial CBV @12:07:39.27
CBV.dial CSV @12:07:40.02 CTV.dial :intake @12:07:40.78 Intake.select :open @12:07:40.90 CS.seek 0ml @12:08:04.39 Intake.select :close @12:08:04.50 CTV.dial :air @12:08:05.25 Flushing upper manifold @12:08:05.39 CSV.dial :flush @12:08:06.14 CS.seek 5ml @12:08:17.71 delay 5 seconds @12:08:22.74 CSV.dial :air @12:08:23.26 CS.seek 7ml @12:08:28.71 CSV.dial CBV @12:08:28.75
CBV.dial CSV @12:08:29.77 CTV.dial :air @12:08:29.79 CS.seek 0ml @12:08:45.61 delay 7 seconds @12:08:52.65 CS.seek 3ml @12:09:00.05 CSV.dial :waste @12:09:00.64 CS.seek 0ml @12:09:08.23 CSV.dial :flush @12:09:08.70 CS.seek 3ml @12:09:16.08 delay 5 seconds @12:09:21.11 CSV.dial :air @12:09:21.64 CS.seek 5ml @12:09:26.99 CSV.dial :air @12:09:27.01 CS.seek 0ml @12:09:38.61 delay 7 seconds @12:09:45.65 CS.seek 3ml @12:09:53.14 CSV.dial :waste @12:09:53.87 CS.seek 0ml @12:10:01.49 Garaging FlushPuck in Collection Clamp @12:10:01.57 Elbow.reconfigure TightElbow @12:10:01.62 Elbow.seek CC @12:10:01.87 Elbow.reconfigure LooseElbow @12:10:01.98 Forearm.seek CC @12:10:03.00 Hand.close @12:10:03.61 CC.seek :open @12:10:07.99 Forearm.seek :retract @12:10:09.00 Elbow.reconfigure TightElbow @12:10:09.05 Elbow.seek :garage @12:10:12.20 Elbow.reconfigure LooseElbow @12:10:12.31 Forearm.reconfigure TightForearm @12:10:12.35 Forearm.seek :garage @12:10:13.50 Hand.open @12:10:14.18 Forearm.reconfigure LooseForearm @12:10:14.23 Forearm.seek :retract @12:10:15.25 Completed [stdphase] begun 10:00:24 @12:10:15.26 Preparing to sleep until Sat May 16 10:00:24 +0200 2020 @12:10:15.32 deferred until MFBPCR finish @12:10:15.34
Waiting for MFBPCR til 10:00CEST16-May-20 @12:10:15.35 Continuing at 10:00CEST16-May-20 @12:10:15.41 GoodNews.email "Then, in tube 2: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:124\n{1 => 9, 2 => 13}\nCan@12:10:15, 8.8C, 43% humidity, 14.3psia, 14.468V, 0.430A, 0.430A avg, 6.23W",Subject: "Waiting until 10:00CEST16-May-20" @12:11:16.25 delay 5 seconds @12:11:21.28 ATV.dial ARV @12:11:22.12 ASV.dial :PCRlock @12:11:22.50 delay 5 seconds @12:11:27.53 Running OM Profile60C350 thermocycling @12:11:28.12 PCR.program Profile60C350 @12:11:28.23 Profile60C350 should complete in < 2:02:45 @12:11:28.24 PCR.run 2:38:26.25 @13:48:12.81 PCR.off @13:48:14.07 PCR for OM completed -- closed /var/log/esp/OM20may1511h275ml.pcr @13:48:14.14 Cleaning PCR @13:48:14.15 AFV.select :carrierSolution @13:48:14.26 delay 2 seconds @13:48:16.29 AS.reconfigure ASconfig @13:48:16.34 AS.seek 0.5ml @13:48:26.65 delay 1 second @13:48:27.67 AFV.select :holdingCoil @13:48:27.77 ASV.dial :waste1 @13:48:28.16 ATV.dial :air3 @13:48:29.38 AS.reconfigure ASslow1 @13:48:29.43 AS.seek 0.51ml @13:48:31.71 delay 2 seconds @13:48:33.73 ATV.dial :bleach @13:48:34.33 AS.seek 0.81ml @13:49:34.89 delay 3 seconds @13:49:37.92 ATV.dial :air3 @13:49:38.45 delay 3 seconds @13:49:41.54 AS.seek 0.835ml @13:49:46.85 delay 3 seconds @13:49:49.88 ATV.dial :bleach @13:49:50.49 AS.seek 0.82ml @13:49:53.80 delay 3 seconds @13:49:56.83 ARV.dial :waste @13:49:57.41 delay 3 seconds @13:50:00.44 ATV.dial :ARV @13:50:01.29 AS.reconfigure ASconfig @13:50:01.34 AS.seek 0.47ml @13:50:21.74 AFV.select :carrierSolution @13:50:21.86 delay 2 seconds @13:50:23.90 AS.seek 1ml @13:50:54.63 delay 1 second @13:50:55.65 AFV.select :holdingCoil @13:50:55.75 delay 1 second @13:50:57.30 AS.reconfigure ASfast @13:50:57.43 AS.seek 0ml @13:51:24.75 AFV.select :carrierSolution @13:51:24.86 delay 2 seconds @13:51:26.89 AS.reconfigure ASconfig @13:51:26.94 AS.seek 1ml @13:52:24.69 delay 1 second @13:52:25.71 AFV.select :holdingCoil @13:52:25.81 delay 1 second @13:52:26.84 AS.reconfigure ASfast @13:52:26.89 AS.seek 0ml @13:52:54.27 AFV.select :carrierSolution @13:52:54.38 delay 2 seconds @13:52:56.41 AS.reconfigure ASconfig @13:52:56.46 AS.seek 1ml @13:53:54.22 delay 1 second @13:53:55.24 AFV.select :holdingCoil @13:53:55.34 delay 1 second @13:53:56.37 AS.reconfigure ASfast @13:53:56.42 AS.seek 0ml @13:54:23.77 ARV.dial between carrier and air1 @13:54:24.25 ATV.dial :air3 @13:54:25.50 AS.reconfigure ASslow1 @13:54:25.55 AS.seek 0.01ml @13:54:27.86 delay 2 seconds @13:54:29.88 ATV.dial :bleach @13:54:30.48 AS.seek 0.81ml @13:57:11.63 delay 3 seconds @13:57:14.66 ATV.dial :air3 @13:57:15.19 delay 3 seconds @13:57:18.28 AS.seek 0.835ml @13:57:23.65 delay 3 seconds @13:57:26.68 ATV.dial :bleach @13:57:27.29 AS.seek 0.82ml @13:57:30.58 delay 3 seconds @13:57:33.61 ATV.dial PCR @13:57:34.01 AS.reconfigure ASconfig @13:57:34.06 AS.seek :empty @13:58:21.52 PCR.program :bleachProfile @13:58:21.58 PCR program should complete in < 2:45 @13:58:21.59 PCR.run 10:00 @14:01:38.56 PCR.off @14:01:38.59 AFV.select :carrierSolution @14:01:38.69 delay 2 seconds @14:01:40.73 AS.seek 1ml @14:02:38.49 delay 1 second @14:02:39.51 AFV.select :holdingCoil @14:02:39.61 delay 1 second @14:02:40.64 AS.reconfigure ASfast @14:02:40.69 AS.seek 0ml @14:03:08.03 delay 3 seconds @14:03:11.06 AFV.select :carrierSolution @14:03:11.16 delay 2 seconds @14:03:13.19 AS.reconfigure ASconfig @14:03:13.25 AS.seek 1ml @14:04:11.01 delay 1 second @14:04:12.03 AFV.select :holdingCoil @14:04:12.13 delay 1 second @14:04:13.16 AS.reconfigure ASfast @14:04:13.21 AS.seek 0ml @14:04:40.53 delay 3 seconds @14:04:43.56 AFV.select :carrierSolution @14:04:43.66 delay 2 seconds @14:04:45.69 AS.reconfigure ASconfig @14:04:45.75 AS.seek 1ml @14:05:43.51 delay 1 second @14:05:44.53 AFV.select :holdingCoil @14:05:44.63 delay 1 second @14:05:45.66 AS.reconfigure ASfast @14:05:45.71 AS.seek 0ml @14:06:13.03 delay 3 seconds @14:06:16.06 AFV.select :carrierSolution @14:06:16.16 delay 2 seconds @14:06:18.19 AS.reconfigure ASconfig @14:06:18.25 AS.seek 1ml @14:07:16.08 delay 1 second @14:07:17.10 AFV.select :holdingCoil @14:07:17.20 delay 1 second @14:07:18.23 AS.reconfigure ASfast @14:07:18.28 AS.seek 0ml @14:07:45.62 delay 3 seconds @14:07:48.65 AFV.select :carrierSolution @14:07:48.75 delay 2 seconds @14:07:50.78 AS.reconfigure ASconfig @14:07:50.84 AS.seek 1ml @14:08:48.65 delay 1 second @14:08:49.67 AFV.select :holdingCoil @14:08:49.77 delay 1 second @14:08:50.80 AS.reconfigure ASnorm @14:08:50.85 AS.seek 0ml @14:09:41.60 Setting up PCR for ML -- writing /var/log/esp/ML20may1514h275ml.pcr @14:09:41.62 Building PCR Slug from ASV with MM2 mix, PP4 primer @14:09:41.63 Priming with carrier @14:09:41.64 ATV.dial ARV @14:09:42.23 ARV.dial :carrier @14:09:42.57 AS.seek 0.5ml @14:10:08.14 AFV.select :carrierSolution @14:10:08.25 delay 2 seconds @14:10:10.28 AS.reconfigure ASconfig @14:10:10.93 AS.seek 1ml @14:10:39.94 delay 1 second @14:10:40.96 AFV.select :holdingCoil @14:10:41.06 delay 1 second @14:10:42.09 ATV.dial :waste @14:10:43.66 AS.reconfigure ASnorm @14:10:43.71 AS.seek :empty @14:11:34.52 AFV.select :carrierSolution @14:11:34.63 delay 2 seconds @14:11:36.66 AS.reconfigure ASconfig @14:11:36.71 AS.seek 0.5ml @14:12:05.78 delay 1 second @14:12:06.80 AFV.select :holdingCoil @14:12:06.90 Building Slug @14:12:06.91 ATV.dial :ARV @14:12:08.47 delay 2 seconds @14:12:10.50 ARV.dial :air1 @14:12:10.89 delay 2 seconds @14:12:12.98 AS.reconfigure ASslow2 @14:12:13.03 AS.seek 0.506ml @14:12:19.45 delay 2 seconds @14:12:21.48 ARV.dial :MM2 @14:12:22.88 delay 2 seconds @14:12:24.91 Adding MM2 @14:12:24.99 AS.seek 0.524ml @14:12:43.47 delay 25 seconds @14:13:08.50 ARV.dial :PP4 @14:13:08.88 Adding PP4 @14:13:08.90 delay 2 seconds @14:13:10.98 AS.seek 0.53ml @14:13:17.22 delay 10 seconds @14:13:27.25 ARV.dial :air3 @14:13:27.63 adding ARV air @14:13:27.65 delay 2 seconds @14:13:29.73 AS.seek 0.536ml @14:13:36.08 delay 5 seconds @14:13:41.11 ARV.dial :carrier @14:13:42.31 delay 7 seconds @14:13:49.40 AS.seek 0.594ml @14:14:47.99 delay 5 seconds @14:14:53.02 ATV.dial :ASV @14:14:53.41 delay 2 seconds @14:14:55.50 AS.seek 0.6ml @14:15:01.94 delay 4 seconds @14:15:05.97 ATV.dial ARV @14:15:06.50 delay 2 seconds @14:15:08.53 ARV.dial :carrier @14:15:08.54 delay 2 seconds @14:15:10.63 AS.seek 0.65ml @14:16:01.33 delay 4 seconds @14:16:05.36 ATV.dial PCR @14:16:05.94 ASV.dial :waste1 @14:16:05.95 ARV.dial :air1 @14:16:06.34 Positioning Slug by 0.324ml @14:16:06.43 AS.seek 0.326ml @14:21:32.46 delay 5 seconds @14:21:37.49 ATV.dial ARV @14:21:38.24 ASV.dial :PCRlock @14:21:38.63 delay 5 seconds @14:21:43.66 Running ML Profile60C350 thermocycling @14:21:43.67 PCR.program Profile60C350 @14:21:43.79 Profile60C350 should complete in < 2:02:45 @14:21:43.80 PCR.run 2:38:26.25 @15:57:55.50 PCR.off @15:57:56.79 PCR for ML completed -- closed /var/log/esp/ML20may1514h275ml.pcr @15:57:56.87 Cleaning PCR @15:57:56.88 AFV.select :carrierSolution @15:57:56.99 delay 2 seconds @15:57:59.02 AS.reconfigure ASconfig @15:57:59.07 AS.seek 0.5ml @15:58:09.38 delay 1 second @15:58:10.40 AFV.select :holdingCoil @15:58:10.50 ASV.dial :waste1 @15:58:10.88 ATV.dial :air3 @15:58:12.12 AS.reconfigure ASslow1 @15:58:12.17 AS.seek 0.51ml @15:58:14.43 delay 2 seconds @15:58:16.45 ATV.dial :bleach @15:58:17.06 AS.seek 0.81ml @15:59:17.63 delay 3 seconds @15:59:20.66 ATV.dial :air3 @15:59:21.19 delay 3 seconds @15:59:24.28 AS.seek 0.835ml @15:59:29.57 delay 3 seconds @15:59:32.60 ATV.dial :bleach @15:59:33.21 AS.seek 0.82ml @15:59:36.66 delay 3 seconds @15:59:39.69 ARV.dial :waste @15:59:40.27 delay 3 seconds @15:59:43.30 ATV.dial :ARV @15:59:44.26 AS.reconfigure ASconfig @15:59:44.31 AS.seek 0.47ml @16:00:04.71 AFV.select :carrierSolution @16:00:04.82 delay 2 seconds @16:00:06.86 AS.seek 1ml @16:00:37.68 delay 1 second @16:00:38.70 AFV.select :holdingCoil @16:00:38.80 delay 1 second @16:00:39.83 AS.reconfigure ASfast @16:00:39.88 AS.seek 0ml @16:01:07.22 AFV.select :carrierSolution @16:01:07.33 delay 2 seconds @16:01:09.36 AS.reconfigure ASconfig @16:01:09.41 AS.seek 1ml @16:02:07.15 delay 1 second @16:02:08.17 AFV.select :holdingCoil @16:02:08.27 delay 1 second @16:02:09.30 AS.reconfigure ASfast @16:02:09.35 AS.seek 0ml @16:02:36.68 AFV.select :carrierSolution @16:02:36.79 delay 2 seconds @16:02:38.82 AS.reconfigure ASconfig @16:02:38.87 AS.seek 1ml @16:03:36.66 delay 1 second @16:03:37.68 AFV.select :holdingCoil @16:03:37.78 delay 1 second @16:03:38.81 AS.reconfigure ASfast @16:03:38.86 AS.seek 0ml @16:04:06.16 ARV.dial between carrier and air1 @16:04:06.63 ATV.dial :air3 @16:04:07.86 AS.reconfigure ASslow1 @16:04:07.91 AS.seek 0.01ml @16:04:10.18 delay 2 seconds @16:04:12.20 ATV.dial :bleach @16:04:12.81 AS.seek 0.81ml @16:06:53.93 delay 3 seconds @16:06:56.96 ATV.dial :air3 @16:06:57.49 delay 3 seconds @16:07:00.58 AS.seek 0.835ml @16:07:05.91 delay 3 seconds @16:07:08.94 ATV.dial :bleach @16:07:09.55 AS.seek 0.82ml @16:07:12.85 delay 3 seconds @16:07:15.87 ATV.dial PCR @16:07:16.26 AS.reconfigure ASconfig @16:07:16.31 AS.seek :empty @16:08:03.76 PCR.program :bleachProfile @16:08:04.40 PCR program should complete in < 2:45 @16:08:04.42 PCR.run 10:00 @16:11:21.38 PCR.off @16:11:21.41 AFV.select :carrierSolution @16:11:21.52 delay 2 seconds @16:11:23.56 AS.seek 1ml @16:12:21.36 delay 1 second @16:12:22.38 AFV.select :holdingCoil @16:12:22.48 delay 1 second @16:12:23.51 AS.reconfigure ASfast @16:12:23.56 AS.seek 0ml @16:12:50.90 delay 3 seconds @16:12:53.93 AFV.select :carrierSolution @16:12:54.03 delay 2 seconds @16:12:56.06 AS.reconfigure ASconfig @16:12:56.11 AS.seek 1ml @16:13:53.92 delay 1 second @16:13:54.94 AFV.select :holdingCoil @16:13:55.04 delay 1 second @16:13:56.07 AS.reconfigure ASfast @16:13:56.12 AS.seek 0ml @16:14:23.47 delay 3 seconds @16:14:26.50 AFV.select :carrierSolution @16:14:26.60 delay 2 seconds @16:14:28.63 AS.reconfigure ASconfig @16:14:28.69 AS.seek 1ml @16:15:26.48 delay 1 second @16:15:27.50 AFV.select :holdingCoil @16:15:27.60 delay 1 second @16:15:28.63 AS.reconfigure ASfast @16:15:28.68 AS.seek 0ml @16:15:56.00 delay 3 seconds @16:15:59.03 AFV.select :carrierSolution @16:15:59.13 delay 2 seconds @16:16:01.16 AS.reconfigure ASconfig @16:16:01.21 AS.seek 1ml @16:16:59.00 delay 1 second @16:17:00.02 AFV.select :holdingCoil @16:17:00.12 delay 1 second @16:17:01.15 AS.reconfigure ASfast @16:17:01.20 AS.seek 0ml @16:17:28.55 delay 3 seconds @16:17:31.58 AFV.select :carrierSolution @16:17:31.68 delay 2 seconds @16:17:33.71 AS.reconfigure ASconfig @16:17:33.77 AS.seek 1ml @16:18:31.56 delay 1 second @16:18:32.58 AFV.select :holdingCoil @16:18:32.68 delay 1 second @16:18:33.71 AS.reconfigure ASnorm @16:18:33.76 AS.seek 0ml @16:19:24.51 Setting up PCR for CG -- writing /var/log/esp/CG20may1516h275ml.pcr @16:19:24.53 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:19:24.54 Priming with carrier @16:19:24.55 ATV.dial ARV @16:19:25.14 ARV.dial :carrier @16:19:25.48 AS.seek 0.5ml @16:19:51.05 AFV.select :carrierSolution @16:19:51.16 delay 2 seconds @16:19:53.19 AS.reconfigure ASconfig @16:19:53.25 AS.seek 1ml @16:20:22.32 delay 1 second @16:20:23.34 AFV.select :holdingCoil @16:20:23.44 delay 1 second @16:20:24.47 ATV.dial :waste @16:20:26.03 AS.reconfigure ASnorm @16:20:26.08 AS.seek :empty @16:21:16.78 AFV.select :carrierSolution @16:21:16.89 delay 2 seconds @16:21:18.92 AS.reconfigure ASconfig @16:21:18.97 AS.seek 0.5ml @16:21:48.08 delay 1 second @16:21:49.10 AFV.select :holdingCoil @16:21:49.20 Building Slug @16:21:49.21 ATV.dial :ARV @16:21:50.77 delay 2 seconds @16:21:52.80 ARV.dial :air1 @16:21:53.19 delay 2 seconds @16:21:55.28 AS.reconfigure ASslow2 @16:21:55.33 AS.seek 0.506ml @16:22:01.57 delay 2 seconds @16:22:03.59 ARV.dial :MM1 @16:22:04.16 delay 2 seconds @16:22:06.19 Adding MM1 @16:22:06.26 AS.seek 0.524ml @16:22:24.55 delay 25 seconds @16:22:49.58 ARV.dial :PP2 @16:22:49.96 Adding PP2 @16:22:49.98 delay 2 seconds @16:22:52.06 AS.seek 0.53ml @16:22:58.38 delay 10 seconds @16:23:08.41 ARV.dial :air2 @16:23:08.79 adding ARV air @16:23:08.81 delay 2 seconds @16:23:10.89 AS.seek 0.536ml @16:23:17.27 delay 5 seconds @16:23:22.30 ARV.dial :carrier @16:23:23.51 delay 7 seconds @16:23:31.20 AS.seek 0.594ml @16:24:29.72 delay 5 seconds @16:24:34.75 ATV.dial :ASV @16:24:35.15 delay 2 seconds @16:24:37.25 AS.seek 0.6ml @16:24:43.69 delay 4 seconds @16:24:47.72 ATV.dial ARV @16:24:48.26 delay 2 seconds @16:24:50.29 ARV.dial :carrier @16:24:50.30 delay 2 seconds @16:24:52.39 AS.seek 0.65ml @16:25:43.12 delay 4 seconds @16:25:47.15 ATV.dial PCR @16:25:47.74 ASV.dial :waste1 @16:25:47.75 ARV.dial :air1 @16:25:48.14 Positioning Slug by 0.324ml @16:25:48.23 AS.seek 0.326ml @16:31:14.35 delay 5 seconds @16:31:19.38 ATV.dial ARV @16:31:20.23 ASV.dial :PCRlock @16:31:20.62 delay 5 seconds @16:31:25.65 Running CG Profile60C300 thermocycling @16:31:25.66 PCR.program Profile60C300 @16:31:25.77 Profile60C300 should complete in < 1:59:25 @16:31:25.78 PCR.run 2:34:16.25 @18:07:01.19 PCR.off @18:07:01.86 PCR for CG completed -- closed /var/log/esp/CG20may1516h275ml.pcr @18:07:01.93 Cleaning PCR @18:07:01.94 AFV.select :carrierSolution @18:07:02.05 delay 2 seconds @18:07:04.08 AS.reconfigure ASconfig @18:07:04.13 AS.seek 0.5ml @18:07:14.44 delay 1 second @18:07:15.46 AFV.select :holdingCoil @18:07:15.56 ASV.dial :waste1 @18:07:15.94 ATV.dial :air3 @18:07:17.14 AS.reconfigure ASslow1 @18:07:17.20 AS.seek 0.51ml @18:07:19.48 delay 2 seconds @18:07:21.50 ATV.dial :bleach @18:07:22.11 AS.seek 0.81ml @18:08:22.68 delay 3 seconds @18:08:25.70 ATV.dial :air3 @18:08:26.24 delay 3 seconds @18:08:29.33 AS.seek 0.835ml @18:08:34.60 delay 3 seconds @18:08:37.62 ATV.dial :bleach @18:08:38.23 AS.seek 0.82ml @18:08:41.52 delay 3 seconds @18:08:44.54 ARV.dial :waste @18:08:45.72 delay 3 seconds @18:08:48.76 ATV.dial :ARV @18:08:49.73 AS.reconfigure ASconfig @18:08:49.78 AS.seek 0.47ml @18:09:10.18 AFV.select :carrierSolution @18:09:10.30 delay 2 seconds @18:09:12.34 AS.seek 1ml @18:09:43.09 delay 1 second @18:09:44.11 AFV.select :holdingCoil @18:09:44.21 delay 1 second @18:09:45.24 AS.reconfigure ASfast @18:09:45.29 AS.seek 0ml @18:10:12.62 AFV.select :carrierSolution @18:10:12.73 delay 2 seconds @18:10:14.76 AS.reconfigure ASconfig @18:10:14.81 AS.seek 1ml @18:11:12.59 delay 1 second @18:11:13.61 AFV.select :holdingCoil @18:11:13.71 delay 1 second @18:11:14.74 AS.reconfigure ASfast @18:11:14.79 AS.seek 0ml @18:11:42.12 AFV.select :carrierSolution @18:11:42.23 delay 2 seconds @18:11:44.26 AS.reconfigure ASconfig @18:11:44.31 AS.seek 1ml @18:12:42.06 delay 1 second @18:12:43.08 AFV.select :holdingCoil @18:12:43.18 delay 1 second @18:12:44.21 AS.reconfigure ASfast @18:12:44.26 AS.seek 0ml @18:13:11.59 ARV.dial between carrier and air1 @18:13:12.07 ATV.dial :air3 @18:13:13.30 AS.reconfigure ASslow1 @18:13:13.35 AS.seek 0.01ml @18:13:15.64 delay 2 seconds @18:13:17.66 ATV.dial :bleach @18:13:18.27 AS.seek 0.81ml @18:15:59.42 delay 3 seconds @18:16:02.45 ATV.dial :air3 @18:16:02.98 delay 3 seconds @18:16:06.07 AS.seek 0.835ml @18:16:11.37 delay 3 seconds @18:16:14.40 ATV.dial :bleach @18:16:15.00 AS.seek 0.82ml @18:16:18.31 delay 3 seconds @18:16:21.33 ATV.dial PCR @18:16:21.73 AS.reconfigure ASconfig @18:16:21.78 AS.seek :empty @18:17:09.26 PCR.program :bleachProfile @18:17:09.32 PCR program should complete in < 2:45 @18:17:09.33 PCR.run 10:00 @18:20:26.30 PCR.off @18:20:26.33 AFV.select :carrierSolution @18:20:26.44 delay 2 seconds @18:20:28.48 AS.seek 1ml @18:21:26.30 delay 1 second @18:21:27.32 AFV.select :holdingCoil @18:21:27.42 delay 1 second @18:21:28.45 AS.reconfigure ASfast @18:21:28.50 AS.seek 0ml @18:21:55.83 delay 3 seconds @18:21:58.86 AFV.select :carrierSolution @18:21:58.96 delay 2 seconds @18:22:01.00 AS.reconfigure ASconfig @18:22:01.05 AS.seek 1ml @18:22:58.80 delay 1 second @18:22:59.82 AFV.select :holdingCoil @18:22:59.92 delay 1 second @18:23:00.95 AS.reconfigure ASfast @18:23:01.00 AS.seek 0ml @18:23:28.31 delay 3 seconds @18:23:31.34 AFV.select :carrierSolution @18:23:31.44 delay 2 seconds @18:23:33.47 AS.reconfigure ASconfig @18:23:33.52 AS.seek 1ml @18:24:31.27 delay 1 second @18:24:32.29 AFV.select :holdingCoil @18:24:32.39 delay 1 second @18:24:33.42 AS.reconfigure ASfast @18:24:33.47 AS.seek 0ml @18:25:00.80 delay 3 seconds @18:25:03.83 AFV.select :carrierSolution @18:25:03.93 delay 2 seconds @18:25:05.96 AS.reconfigure ASconfig @18:25:06.02 AS.seek 1ml @18:26:03.82 delay 1 second @18:26:04.84 AFV.select :holdingCoil @18:26:04.94 delay 1 second @18:26:05.97 AS.reconfigure ASfast @18:26:06.02 AS.seek 0ml @18:26:33.35 delay 3 seconds @18:26:36.38 AFV.select :carrierSolution @18:26:36.48 delay 2 seconds @18:26:38.51 AS.reconfigure ASconfig @18:26:38.56 AS.seek 1ml @18:27:36.40 delay 1 second @18:27:37.42 AFV.select :holdingCoil @18:27:37.52 delay 1 second @18:27:38.55 AS.reconfigure ASnorm @18:27:38.60 AS.seek 0ml @18:28:29.31 Setting up PCR for NM -- writing /var/log/esp/NM20may1518h275ml.pcr @18:28:29.91 Building PCR Slug from ASV with MM1 mix, PP1 primer @18:28:29.94 Priming with carrier @18:28:29.95 ATV.dial ARV @18:28:30.54 ARV.dial :carrier @18:28:30.89 AS.seek 0.5ml @18:28:56.46 AFV.select :carrierSolution @18:28:56.57 delay 2 seconds @18:28:58.60 AS.reconfigure ASconfig @18:28:58.65 AS.seek 1ml @18:29:27.71 delay 1 second @18:29:28.73 AFV.select :holdingCoil @18:29:28.83 delay 1 second @18:29:29.86 ATV.dial :waste @18:29:31.43 AS.reconfigure ASnorm @18:29:31.48 AS.seek :empty @18:30:22.23 AFV.select :carrierSolution @18:30:22.34 delay 2 seconds @18:30:24.37 AS.reconfigure ASconfig @18:30:24.42 AS.seek 0.5ml @18:30:53.51 delay 1 second @18:30:54.53 AFV.select :holdingCoil @18:30:54.63 Building Slug @18:30:54.64 ATV.dial :ARV @18:30:56.20 delay 2 seconds @18:30:58.23 ARV.dial :air1 @18:30:58.63 delay 2 seconds @18:31:00.72 AS.reconfigure ASslow2 @18:31:00.77 AS.seek 0.506ml @18:31:07.02 delay 2 seconds @18:31:09.04 ARV.dial :MM1 @18:31:09.60 delay 2 seconds @18:31:11.63 Adding MM1 @18:31:11.70 AS.seek 0.524ml @18:31:29.91 delay 25 seconds @18:31:54.94 ARV.dial :PP1 @18:31:55.32 Adding PP1 @18:31:55.34 delay 2 seconds @18:31:57.42 AS.seek 0.53ml @18:32:03.62 delay 10 seconds @18:32:13.65 ARV.dial :air1 @18:32:14.03 adding ARV air @18:32:14.05 delay 2 seconds @18:32:16.13 AS.seek 0.536ml @18:32:22.40 delay 5 seconds @18:32:27.43 ARV.dial :carrier @18:32:27.79 delay 7 seconds @18:32:34.88 AS.seek 0.594ml @18:33:33.59 delay 5 seconds @18:33:38.62 ATV.dial :ASV @18:33:39.01 delay 2 seconds @18:33:41.10 AS.seek 0.6ml @18:33:47.32 delay 4 seconds @18:33:51.35 ATV.dial ARV @18:33:51.89 delay 2 seconds @18:33:53.92 ARV.dial :carrier @18:33:53.93 delay 2 seconds @18:33:56.02 AS.seek 0.65ml @18:34:46.51 delay 4 seconds @18:34:50.54 ATV.dial PCR @18:34:51.12 ASV.dial :waste1 @18:34:51.13 ARV.dial :air1 @18:34:51.52 Positioning Slug by 0.324ml @18:34:51.61 AS.seek 0.326ml @18:40:17.70 delay 5 seconds @18:40:22.73 ATV.dial ARV @18:40:23.48 ASV.dial :PCRlock @18:40:23.86 delay 5 seconds @18:40:28.89 Running NM Profile60C300 thermocycling @18:40:28.90 PCR.program Profile60C300 @18:40:29.01 Profile60C300 should complete in < 1:59:25 @18:40:29.02 PCR.run 2:34:16.25 @20:15:22.84 PCR.off @20:15:23.50 PCR for NM completed -- closed /var/log/esp/NM20may1518h275ml.pcr @20:15:23.57 Cleaning PCR @20:15:23.58 AFV.select :carrierSolution @20:15:23.69 delay 2 seconds @20:15:25.72 AS.reconfigure ASconfig @20:15:25.77 AS.seek 0.5ml @20:15:36.07 delay 1 second @20:15:37.09 AFV.select :holdingCoil @20:15:37.19 ASV.dial :waste1 @20:15:37.57 ATV.dial :air3 @20:15:38.80 AS.reconfigure ASslow1 @20:15:38.85 AS.seek 0.51ml @20:15:41.10 delay 2 seconds @20:15:43.12 ATV.dial :bleach @20:15:43.72 AS.seek 0.81ml @20:16:44.31 delay 3 seconds @20:16:47.34 ATV.dial :air3 @20:16:47.87 delay 3 seconds @20:16:50.96 AS.seek 0.835ml @20:16:56.24 delay 3 seconds @20:16:59.27 ATV.dial :bleach @20:16:59.88 AS.seek 0.82ml @20:17:03.17 delay 3 seconds @20:17:06.20 ARV.dial :waste @20:17:06.78 delay 3 seconds @20:17:09.81 ATV.dial :ARV @20:17:10.76 AS.reconfigure ASconfig @20:17:10.82 AS.seek 0.47ml @20:17:31.21 AFV.select :carrierSolution @20:17:31.33 delay 2 seconds @20:17:33.37 AS.seek 1ml @20:18:04.12 delay 1 second @20:18:05.14 AFV.select :holdingCoil @20:18:05.24 delay 1 second @20:18:06.27 AS.reconfigure ASfast @20:18:06.32 AS.seek 0ml @20:18:33.67 AFV.select :carrierSolution @20:18:33.78 delay 2 seconds @20:18:35.81 AS.reconfigure ASconfig @20:18:35.86 AS.seek 1ml @20:19:33.65 delay 1 second @20:19:34.67 AFV.select :holdingCoil @20:19:34.77 delay 1 second @20:19:35.80 AS.reconfigure ASfast @20:19:35.85 AS.seek 0ml @20:20:03.17 AFV.select :carrierSolution @20:20:03.28 delay 2 seconds @20:20:05.31 AS.reconfigure ASconfig @20:20:05.36 AS.seek 1ml @20:21:03.14 delay 1 second @20:21:04.16 AFV.select :holdingCoil @20:21:04.26 delay 1 second @20:21:05.29 AS.reconfigure ASfast @20:21:05.34 AS.seek 0ml @20:21:32.69 ARV.dial between carrier and air1 @20:21:33.17 ATV.dial :air3 @20:21:34.40 AS.reconfigure ASslow1 @20:21:34.45 AS.seek 0.01ml @20:21:36.73 delay 2 seconds @20:21:38.75 ATV.dial :bleach @20:21:39.35 AS.seek 0.81ml @20:24:20.49 delay 3 seconds @20:24:23.51 ATV.dial :air3 @20:24:24.05 delay 3 seconds @20:24:27.14 AS.seek 0.835ml @20:24:32.43 delay 3 seconds @20:24:35.45 ATV.dial :bleach @20:24:36.06 AS.seek 0.82ml @20:24:39.35 delay 3 seconds @20:24:42.38 ATV.dial PCR @20:24:42.77 AS.reconfigure ASconfig @20:24:42.82 AS.seek :empty @20:25:30.27 PCR.program :bleachProfile @20:25:30.33 PCR program should complete in < 2:45 @20:25:30.34 PCR.run 10:00 @20:28:44.87 PCR.off @20:28:44.90 AFV.select :carrierSolution @20:28:45.01 delay 2 seconds @20:28:47.05 AS.seek 1ml @20:29:44.88 delay 1 second @20:29:45.90 AFV.select :holdingCoil @20:29:46.00 delay 1 second @20:29:47.03 AS.reconfigure ASfast @20:29:47.08 AS.seek 0ml @20:30:14.43 delay 3 seconds @20:30:17.46 AFV.select :carrierSolution @20:30:17.56 delay 2 seconds @20:30:19.59 AS.reconfigure ASconfig @20:30:19.65 AS.seek 1ml @20:31:17.40 delay 1 second @20:31:18.42 AFV.select :holdingCoil @20:31:18.52 delay 1 second @20:31:19.55 AS.reconfigure ASfast @20:31:19.60 AS.seek 0ml @20:31:46.93 delay 3 seconds @20:31:49.96 AFV.select :carrierSolution @20:31:50.06 delay 2 seconds @20:31:52.09 AS.reconfigure ASconfig @20:31:52.14 AS.seek 1ml @20:32:49.93 delay 1 second @20:32:50.95 AFV.select :holdingCoil @20:32:51.05 delay 1 second @20:32:52.08 AS.reconfigure ASfast @20:32:52.13 AS.seek 0ml @20:33:19.51 delay 3 seconds @20:33:22.54 AFV.select :carrierSolution @20:33:22.64 delay 2 seconds @20:33:24.67 AS.reconfigure ASconfig @20:33:24.73 AS.seek 1ml @20:34:22.57 delay 1 second @20:34:23.59 AFV.select :holdingCoil @20:34:23.69 delay 1 second @20:34:24.72 AS.reconfigure ASfast @20:34:24.77 AS.seek 0ml @20:34:52.10 delay 3 seconds @20:34:55.13 AFV.select :carrierSolution @20:34:55.23 delay 2 seconds @20:34:57.26 AS.reconfigure ASconfig @20:34:57.32 AS.seek 1ml @20:35:55.04 delay 1 second @20:35:56.06 AFV.select :holdingCoil @20:35:56.16 delay 1 second @20:35:57.19 AS.reconfigure ASnorm @20:35:57.24 AS.seek 0ml @20:36:47.97 Setting up PCR for IPc -- writing /var/log/esp/IPc20may1520h275ml.pcr @20:36:47.98 Building PCR Slug from ASV with MM3 mix, PP6 primer @20:36:48.00 Priming with carrier @20:36:48.01 ATV.dial ARV @20:36:48.59 ARV.dial :carrier @20:36:48.94 AS.seek 0.5ml @20:37:14.49 AFV.select :carrierSolution @20:37:14.61 delay 2 seconds @20:37:16.64 AS.reconfigure ASconfig @20:37:16.69 AS.seek 1ml @20:37:45.72 delay 1 second @20:37:46.74 AFV.select :holdingCoil @20:37:46.84 delay 1 second @20:37:47.87 ATV.dial :waste @20:37:49.43 AS.reconfigure ASnorm @20:37:49.48 AS.seek :empty @20:38:40.22 AFV.select :carrierSolution @20:38:40.33 delay 2 seconds @20:38:42.36 AS.reconfigure ASconfig @20:38:42.41 AS.seek 0.5ml @20:39:11.48 delay 1 second @20:39:12.50 AFV.select :holdingCoil @20:39:12.60 Building Slug @20:39:12.61 ATV.dial :ARV @20:39:14.17 delay 2 seconds @20:39:16.20 ARV.dial :air1 @20:39:16.59 delay 2 seconds @20:39:18.68 AS.reconfigure ASslow2 @20:39:18.73 AS.seek 0.506ml @20:39:24.89 delay 2 seconds @20:39:26.91 ARV.dial :MM3 @20:39:27.92 delay 2 seconds @20:39:29.95 Adding MM3 @20:39:30.03 AS.seek 0.524ml @20:39:48.37 delay 25 seconds @20:40:13.40 ARV.dial :PP6 @20:40:13.78 Adding PP6 @20:40:13.79 delay 2 seconds @20:40:15.88 AS.seek 0.53ml @20:40:22.22 delay 10 seconds @20:40:32.25 ARV.dial :air1 @20:40:33.03 adding ARV air @20:40:33.05 delay 2 seconds @20:40:35.13 AS.seek 0.536ml @20:40:41.51 delay 5 seconds @20:40:46.54 ARV.dial :carrier @20:40:46.94 delay 7 seconds @20:40:54.03 AS.seek 0.594ml @20:41:52.50 delay 5 seconds @20:41:57.53 ATV.dial :ASV @20:41:57.92 delay 2 seconds @20:42:00.01 AS.seek 0.6ml @20:42:06.92 delay 4 seconds @20:42:10.95 ATV.dial ARV @20:42:11.48 delay 2 seconds @20:42:13.51 ARV.dial :carrier @20:42:13.52 delay 2 seconds @20:42:15.61 AS.seek 0.65ml @20:43:06.30 delay 4 seconds @20:43:10.33 ATV.dial PCR @20:43:10.92 ASV.dial :waste1 @20:43:10.93 ARV.dial :air1 @20:43:11.32 Positioning Slug by 0.324ml @20:43:11.41 AS.seek 0.326ml @20:48:37.51 delay 5 seconds @20:48:42.54 ATV.dial ARV @20:48:43.29 ASV.dial :PCRlock @20:48:43.68 delay 5 seconds @20:48:48.71 Running IPc ProfileIPc thermocycling @20:48:48.72 PCR.program ProfileIPc @20:48:48.83 ProfileIPc should complete in < 1:36:15 @20:48:48.84 PCR.run 2:05:18.75 @22:06:57.70 PCR.off @22:06:58.25 PCR for IPc completed -- closed /var/log/esp/IPc20may1520h275ml.pcr @22:06:58.32 Cleaning PCR @22:06:58.33 AFV.select :carrierSolution @22:06:58.44 delay 2 seconds @22:07:00.47 AS.reconfigure ASconfig @22:07:00.52 AS.seek 0.5ml @22:07:10.83 delay 1 second @22:07:11.85 AFV.select :holdingCoil @22:07:11.95 ASV.dial :waste1 @22:07:12.33 ATV.dial :air3 @22:07:13.56 AS.reconfigure ASslow1 @22:07:13.61 AS.seek 0.51ml @22:07:15.89 delay 2 seconds @22:07:17.91 ATV.dial :bleach @22:07:19.13 AS.seek 0.81ml @22:08:19.72 delay 3 seconds @22:08:22.75 ATV.dial :air3 @22:08:23.29 delay 3 seconds @22:08:26.38 AS.seek 0.835ml @22:08:31.71 delay 3 seconds @22:08:34.74 ATV.dial :bleach @22:08:35.34 AS.seek 0.82ml @22:08:38.63 delay 3 seconds @22:08:41.66 ARV.dial :waste @22:08:42.24 delay 3 seconds @22:08:45.27 ATV.dial :ARV @22:08:46.12 AS.reconfigure ASconfig @22:08:46.17 AS.seek 0.47ml @22:09:06.55 AFV.select :carrierSolution @22:09:06.66 delay 2 seconds @22:09:08.70 AS.seek 1ml @22:09:39.46 delay 1 second @22:09:40.48 AFV.select :holdingCoil @22:09:40.58 delay 1 second @22:09:41.61 AS.reconfigure ASfast @22:09:41.66 AS.seek 0ml @22:10:08.99 AFV.select :carrierSolution @22:10:09.10 delay 2 seconds @22:10:11.13 AS.reconfigure ASconfig @22:10:11.18 AS.seek 1ml @22:11:08.94 delay 1 second @22:11:09.96 AFV.select :holdingCoil @22:11:10.06 delay 1 second @22:11:11.09 AS.reconfigure ASfast @22:11:11.14 AS.seek 0ml @22:11:38.45 AFV.select :carrierSolution @22:11:38.56 delay 2 seconds @22:11:40.59 AS.reconfigure ASconfig @22:11:40.64 AS.seek 1ml @22:12:38.41 delay 1 second @22:12:39.43 AFV.select :holdingCoil @22:12:39.53 delay 1 second @22:12:40.56 AS.reconfigure ASfast @22:12:40.61 AS.seek 0ml @22:13:07.95 ARV.dial between carrier and air1 @22:13:08.43 ATV.dial :air3 @22:13:09.67 AS.reconfigure ASslow1 @22:13:09.72 AS.seek 0.01ml @22:13:12.02 delay 2 seconds @22:13:14.04 ATV.dial :bleach @22:13:14.64 AS.seek 0.81ml @22:15:55.79 delay 3 seconds @22:15:58.82 ATV.dial :air3 @22:15:59.35 delay 3 seconds @22:16:02.44 AS.seek 0.835ml @22:16:07.73 delay 3 seconds @22:16:10.75 ATV.dial :bleach @22:16:11.36 AS.seek 0.82ml @22:16:14.67 delay 3 seconds @22:16:17.70 ATV.dial PCR @22:16:18.09 AS.reconfigure ASconfig @22:16:18.14 AS.seek :empty @22:17:05.61 PCR.program :bleachProfile @22:17:05.67 PCR program should complete in < 2:45 @22:17:05.68 PCR.run 10:00 @22:20:22.65 PCR.off @22:20:22.68 AFV.select :carrierSolution @22:20:22.78 delay 2 seconds @22:20:24.82 AS.seek 1ml @22:21:22.66 delay 1 second @22:21:23.68 AFV.select :holdingCoil @22:21:23.78 delay 1 second @22:21:24.81 AS.reconfigure ASfast @22:21:24.86 AS.seek 0ml @22:21:52.19 delay 3 seconds @22:21:55.21 AFV.select :carrierSolution @22:21:55.31 delay 2 seconds @22:21:57.34 AS.reconfigure ASconfig @22:21:57.39 AS.seek 1ml @22:22:55.19 delay 1 second @22:22:56.21 AFV.select :holdingCoil @22:22:56.31 delay 1 second @22:22:57.34 AS.reconfigure ASfast @22:22:57.39 AS.seek 0ml @22:23:24.72 delay 3 seconds @22:23:27.75 AFV.select :carrierSolution @22:23:27.85 delay 2 seconds @22:23:29.88 AS.reconfigure ASconfig @22:23:29.94 AS.seek 1ml @22:24:27.75 delay 1 second @22:24:28.77 AFV.select :holdingCoil @22:24:28.87 delay 1 second @22:24:29.90 AS.reconfigure ASfast @22:24:29.95 AS.seek 0ml @22:24:57.28 delay 3 seconds @22:25:00.31 AFV.select :carrierSolution @22:25:00.41 delay 2 seconds @22:25:02.44 AS.reconfigure ASconfig @22:25:02.49 AS.seek 1ml @22:26:00.82 delay 1 second @22:26:01.84 AFV.select :holdingCoil @22:26:01.94 delay 1 second @22:26:02.97 AS.reconfigure ASfast @22:26:03.02 AS.seek 0ml @22:26:30.35 delay 3 seconds @22:26:33.38 AFV.select :carrierSolution @22:26:33.48 delay 2 seconds @22:26:35.51 AS.reconfigure ASconfig @22:26:35.56 AS.seek 1ml @22:27:33.30 delay 1 second @22:27:34.32 AFV.select :holdingCoil @22:27:34.42 delay 1 second @22:27:35.45 AS.reconfigure ASnorm @22:27:35.50 AS.seek 0ml @22:28:26.23 Setting up PCR for IPc2 -- writing /var/log/esp/IPc220may1522h275ml.pcr @22:28:26.25 Building PCR Slug from ASV with MM2 mix, PP3 primer @22:28:26.26 Priming with carrier @22:28:26.27 ATV.dial ARV @22:28:26.86 ARV.dial :carrier @22:28:27.20 AS.seek 0.5ml @22:28:52.79 AFV.select :carrierSolution @22:28:52.90 delay 2 seconds @22:28:54.93 AS.reconfigure ASconfig @22:28:54.98 AS.seek 1ml @22:29:23.99 delay 1 second @22:29:25.01 AFV.select :holdingCoil @22:29:25.11 delay 1 second @22:29:26.14 ATV.dial :waste @22:29:27.71 AS.reconfigure ASnorm @22:29:27.76 AS.seek :empty @22:30:18.48 AFV.select :carrierSolution @22:30:18.59 delay 2 seconds @22:30:20.62 AS.reconfigure ASconfig @22:30:20.67 AS.seek 0.5ml @22:30:49.73 delay 1 second @22:30:50.75 AFV.select :holdingCoil @22:30:50.85 Building Slug @22:30:50.86 ATV.dial :ARV @22:30:52.41 delay 2 seconds @22:30:54.44 ARV.dial :air1 @22:30:54.83 delay 2 seconds @22:30:56.92 AS.reconfigure ASslow2 @22:30:56.97 AS.seek 0.506ml @22:31:03.35 delay 2 seconds @22:31:05.37 ARV.dial :MM2 @22:31:06.77 delay 2 seconds @22:31:08.80 Adding MM2 @22:31:08.87 AS.seek 0.524ml @22:31:27.46 delay 25 seconds @22:31:52.49 ARV.dial :PP3 @22:31:52.87 Adding PP3 @22:31:52.88 delay 2 seconds @22:31:54.97 AS.seek 0.53ml @22:32:01.23 delay 10 seconds @22:32:11.26 ARV.dial :air2 @22:32:11.63 adding ARV air @22:32:11.64 delay 2 seconds @22:32:13.73 AS.seek 0.536ml @22:32:19.88 delay 5 seconds @22:32:24.91 ARV.dial :carrier @22:32:26.12 delay 7 seconds @22:32:33.21 AS.seek 0.594ml @22:33:31.79 delay 5 seconds @22:33:36.82 ATV.dial :ASV @22:33:37.21 delay 2 seconds @22:33:39.30 AS.seek 0.6ml @22:33:45.49 delay 4 seconds @22:33:49.52 ATV.dial ARV @22:33:50.06 delay 2 seconds @22:33:52.09 ARV.dial :carrier @22:33:52.10 delay 2 seconds @22:33:54.19 AS.seek 0.65ml @22:34:44.68 delay 4 seconds @22:34:48.71 ATV.dial PCR @22:34:49.29 ASV.dial :waste1 @22:34:49.30 ARV.dial :air1 @22:34:49.69 Positioning Slug by 0.324ml @22:34:49.78 AS.seek 0.326ml @22:40:15.62 delay 5 seconds @22:40:20.65 ATV.dial ARV @22:40:21.50 ASV.dial :PCRlock @22:40:21.89 delay 5 seconds @22:40:26.92 Running IPc2 ProfileIPc thermocycling @22:40:26.94 PCR.program ProfileIPc @22:40:27.04 ProfileIPc should complete in < 1:36:15 @22:40:27.05 PCR.run 2:05:18.75 @23:58:35.83 PCR.off @23:58:36.38 PCR for IPc2 completed -- closed /var/log/esp/IPc220may1522h275ml.pcr @23:58:36.40 Full MFB Decontamination @23:58:36.41 Decon extraction column and decon AIV @23:58:36.43 SPE.hold 2:46:40,55C @23:58:36.47 ASV.dial :SPEdelivery @23:58:37.09 delay 20 seconds @23:58:57.12 AFV.select :carrierSolution @23:58:57.22 delay 2 seconds @23:58:59.25 AS.reconfigure ASconfig @23:58:59.30 AS.seek 0.5ml @23:59:09.59 delay 1 second @23:59:10.61 AFV.select :holdingCoil @23:59:10.71 ATV.dial :air1 @23:59:12.40 AS.reconfigure ASslow1 @23:59:12.45 AS.seek 0.52ml @23:59:16.79 ATV.dial :ASV @23:59:18.36 ASV.dial :SPErecovery @23:59:19.01 AS.seek 0.62ml @23:59:39.42 ATV.dial :waste @23:59:41.17 AS.reconfigure ASnorm @23:59:41.22 AS.seek :empty @00:00:12.81CEST16-May-20 AFV.select :holdingCoil @00:00:12.82 ATV.dial :waste @00:00:12.84 AS.reconfigure ASconfig @00:00:12.89 AS.seek 0ml @00:00:13.23 AFV.select :carrierSolution @00:00:13.34 delay 2 seconds @00:00:15.38 AS.seek 0.5ml @00:00:44.40 delay 1 second @00:00:45.42 AFV.select :holdingCoil @00:00:45.52 ATV.dial :air3 @00:00:46.19 AS.reconfigure ASslow1 @00:00:46.24 AS.seek 0.52ml @00:00:50.51 ATV.dial :air3 @00:00:50.59 AS.seek 0.53ml @00:00:52.86 delay 2 seconds @00:00:54.88 ATV.dial :bleach @00:00:55.33 AS.seek 0.71ml @00:01:31.78 delay 3 seconds @00:01:34.81 ATV.dial :air3 @00:01:35.34 delay 3 seconds @00:01:38.43 AS.seek 0.735ml @00:01:43.72 delay 3 seconds @00:01:46.75 ATV.dial :bleach @00:01:47.98 AS.seek 0.72ml @00:01:51.27 delay 3 seconds @00:01:54.30 ATV.dial ASV @00:01:55.30 ASV.dial :SPErecovery @00:01:55.39 AS.seek 0.365ml @00:03:07.05 ATV.dial :waste @00:03:08.66 AS.reconfigure ASnorm @00:03:08.72 AS.seek :empty @00:03:27.50 ATV.dial :air3 @00:03:28.18 AS.reconfigure ASslow1 @00:03:28.23 AS.seek 0.009ml @00:03:30.53 delay 2 seconds @00:03:32.55 ATV.dial :bleach @00:03:33.01 AS.seek 0.579ml @00:05:27.89 delay 3 seconds @00:05:30.92 ATV.dial :air3 @00:05:31.46 delay 3 seconds @00:05:34.55 AS.seek 0.604ml @00:05:39.87 delay 3 seconds @00:05:42.90 ATV.dial :bleach @00:05:43.49 AS.seek 0.589ml @00:05:46.78 delay 3 seconds @00:05:49.81 ATV.dial :ASV @00:05:50.80 ASV.dial :wasteR @00:05:51.20 AS.seek 0ml @00:07:49.94 delay 1:00 @00:08:49.97 ATV.dial ASV @00:08:49.98 AFV.select :carrierSolution @00:08:50.08 delay 2 seconds @00:08:52.11 AS.reconfigure ASconfig @00:08:52.16 AS.seek 1ml @00:09:49.93 delay 1 second @00:09:50.95 AFV.select :holdingCoil @00:09:51.05 delay 1 second @00:09:52.08 ASV.dial :wasteR @00:09:52.10 AS.reconfigure ASslow1 @00:09:52.14 AS.seek 0ml @00:13:13.51 delay 10 seconds @00:13:23.54 ATV.dial :air3 @00:13:24.97 AS.seek 0.01ml @00:13:27.26 delay 2 seconds @00:13:29.28 ATV.dial :bleach @00:13:29.91 AS.seek 0.58ml @00:15:24.79 delay 3 seconds @00:15:27.82 ATV.dial :air3 @00:15:28.35 delay 3 seconds @00:15:31.44 AS.seek 0.605ml @00:15:36.71 delay 3 seconds @00:15:39.74 ATV.dial :bleach @00:15:40.35 AS.seek 0.59ml @00:15:43.67 delay 3 seconds @00:15:46.70 ATV.dial ASV @00:15:47.70 ASV.dial :SPEdelivery @00:15:48.08 AS.seek 0ml @00:17:46.96 delay 1:00 @00:18:46.99 AFV.select :carrierSolution @00:18:47.09 delay 2 seconds @00:18:49.12 AS.reconfigure ASconfig @00:18:49.17 AS.seek 1ml @00:19:46.97 delay 1 second @00:19:47.99 AFV.select :holdingCoil @00:19:48.09 delay 1 second @00:19:49.12 ATV.dial ASV @00:19:49.13 ASV.dial :SPEdelivery @00:19:49.15 AS.reconfigure ASslow1 @00:19:49.19 AS.seek 0ml @00:23:10.58 delay 10 seconds @00:23:20.61 ATV.dial :air2 @00:23:21.40 AS.reconfigure ASslow0 @00:23:21.45 AS.seek 0.8ml @00:24:42.20 delay 3 seconds @00:24:45.23 ATV.dial :ASV @00:24:46.16 ASV.dial :SPEdelivery @00:24:46.18 AS.reconfigure ASslow1 @00:24:46.23 AS.seek 0.03ml @00:27:21.35 delay 20 seconds @00:27:41.38 ATV.dial :waste,via: :air3 @00:27:43.00 AS.reconfigure ASconfig @00:27:43.05 AS.seek :empty @00:27:45.13 SPE.coast @00:27:45.22 Removing Bleach from Mixing Coil @00:27:45.23 AFV.select :carrierSolution @00:27:45.34 delay 2 seconds @00:27:47.38 AS.seek 0.5ml @00:28:16.47 delay 1 second @00:28:17.49 AFV.select :holdingCoil @00:28:17.59 ATV.dial :ASV @00:28:19.19 ASV.dial :SPErecovery @00:28:19.84 AS.reconfigure ASslow1 @00:28:19.89 AS.seek 0.73ml @00:29:06.99 ASV.dial :wasteD @00:29:07.65 AS.reconfigure ASnorm @00:29:07.70 AS.seek 0ml @00:29:44.83 AFV.select :carrierSolution @00:29:44.94 delay 2 seconds @00:29:46.97 AS.reconfigure ASconfig @00:29:47.02 AS.seek 0.5ml @00:30:16.07 delay 1 second @00:30:17.09 AFV.select :holdingCoil @00:30:17.19 ATV.dial ASV @00:30:17.20 ASV.dial :SPErecovery @00:30:17.84 AS.reconfigure ASslow0 @00:30:17.89 AS.seek 0.3ml @00:30:38.36 ATV.dial :air2 @00:30:39.36 AS.reconfigure ASslow1 @00:30:39.41 AS.seek 0.32ml @00:30:43.72 ATV.dial ASV @00:30:44.71 AS.reconfigure ASslow0 @00:30:44.76 AS.seek 0.57ml @00:31:10.19 ASV.dial :wasteD @00:31:10.75 AS.reconfigure ASnorm @00:31:10.81 AS.seek 0ml @00:31:39.90 AFV.select :carrierSolution @00:31:40.01 delay 2 seconds @00:31:42.04 AS.reconfigure ASconfig @00:31:42.09 AS.seek 0.5ml @00:32:11.11 delay 1 second @00:32:12.13 AFV.select :holdingCoil @00:32:12.23 ATV.dial ASV @00:32:12.24 ASV.dial :SPErecovery @00:32:12.89 AS.reconfigure ASslow0 @00:32:12.94 AS.seek 0.3ml @00:32:33.41 ATV.dial :air2 @00:32:34.42 AS.reconfigure ASslow1 @00:32:34.47 AS.seek 0.32ml @00:32:38.78 ATV.dial ASV @00:32:39.79 AS.reconfigure ASslow0 @00:32:39.84 AS.seek 0.57ml @00:33:05.22 ASV.dial :wasteD @00:33:05.79 AS.reconfigure ASnorm @00:33:05.84 AS.seek 0ml @00:33:34.88 ATV.dial :air2 @00:33:35.82 AS.reconfigure ASslow0 @00:33:35.87 AS.seek 0.3ml @00:34:06.38 ATV.dial :ASV @00:34:07.32 ASV.dial :wasteD @00:34:07.34 AS.reconfigure ASconfig @00:34:07.39 AS.seek 0.05ml @00:34:22.08 ATV.dial :waste @00:34:23.84 AS.seek 0ml @00:34:26.99 ASV.dial :SPEdelivery @00:34:27.94 deconLysateINT @00:34:27.95 ATV.dial :air3 @00:34:28.62 AS.reconfigure ASslow1 @00:34:28.67 AS.seek 0.01ml @00:34:31.01 delay 2 seconds @00:34:33.03 ATV.dial :bleach @00:34:33.48 AS.seek 0.96ml @00:37:44.77 delay 3 seconds @00:37:47.80 ATV.dial :air3 @00:37:48.34 delay 3 seconds @00:37:51.43 AS.seek 0.985ml @00:37:57.23 delay 3 seconds @00:38:00.26 ATV.dial :bleach @00:38:00.86 AS.seek 0.97ml @00:38:04.13 delay 3 seconds @00:38:07.16 ATV.dial AIV @00:38:08.35 AIV.dial :coilToMFB @00:38:08.36 AWV.select :waste @00:38:08.38 AFV.select :carrierSolution @00:38:08.49 delay 2 seconds @00:38:10.52 AS.reconfigure ASconfig @00:38:10.57 AS.seek 1ml @00:38:12.62 delay 1 second @00:38:13.64 AFV.select :holdingCoil @00:38:13.74 delay 1 second @00:38:14.77 AS.reconfigure ASslow0 @00:38:14.82 AS.seek :empty @00:39:55.82 AFV.select :carrierSolution @00:39:55.93 delay 2 seconds @00:39:57.96 AS.reconfigure ASconfig @00:39:58.01 AS.seek 1ml @00:40:55.82 delay 1 second @00:40:56.84 AFV.select :holdingCoil @00:40:56.94 delay 1 second @00:40:57.97 AS.reconfigure ASslow0 @00:40:58.02 AS.seek :empty @00:42:38.98 AFV.select :carrierSolution @00:42:39.09 delay 2 seconds @00:42:41.70 AS.reconfigure ASconfig @00:42:41.77 AS.seek 1ml @00:43:39.59 delay 1 second @00:43:40.61 AFV.select :holdingCoil @00:43:40.71 delay 1 second @00:43:41.74 AS.reconfigure ASslow0 @00:43:41.79 AS.seek :empty @00:45:22.78 ATV.dial :air2 @00:45:23.44 AS.reconfigure ASslow1 @00:45:23.49 AS.seek 0.009ml @00:45:25.78 AS.reconfigure ASslow0 @00:45:25.83 AS.seek 1ml @00:47:05.78 ATV.dial AIV @00:47:06.54 AS.seek 0.02ml @00:48:45.39 ATV.dial :waste @00:48:46.96 AS.seek 0ml @00:48:49.28 AWV.select :air @00:48:49.44 Cleaning PCR @00:48:49.46 AFV.select :carrierSolution @00:48:49.56 delay 2 seconds @00:48:51.59 AS.reconfigure ASconfig @00:48:51.64 AS.seek 0.5ml @00:49:20.67 delay 1 second @00:49:21.69 AFV.select :holdingCoil @00:49:21.79 ASV.dial :waste1 @00:49:22.16 ATV.dial :air3 @00:49:22.82 AS.reconfigure ASslow1 @00:49:22.87 AS.seek 0.51ml @00:49:25.13 delay 2 seconds @00:49:27.15 ATV.dial :bleach @00:49:27.62 AS.seek 0.81ml @00:50:28.20 delay 3 seconds @00:50:31.23 ATV.dial :air3 @00:50:31.77 delay 3 seconds @00:50:34.86 AS.seek 0.835ml @00:50:40.17 delay 3 seconds @00:50:43.20 ATV.dial :bleach @00:50:43.81 AS.seek 0.82ml @00:50:47.06 delay 3 seconds @00:50:50.09 ARV.dial :waste @00:50:50.67 delay 3 seconds @00:50:53.70 ATV.dial :ARV @00:50:54.55 AS.reconfigure ASconfig @00:50:54.61 AS.seek 0.47ml @00:51:15.02 AFV.select :carrierSolution @00:51:15.13 delay 2 seconds @00:51:17.17 AS.seek 1ml @00:51:47.96 delay 1 second @00:51:48.98 AFV.select :holdingCoil @00:51:49.08 delay 1 second @00:51:50.11 AS.reconfigure ASfast @00:51:50.16 AS.seek 0ml @00:52:17.49 AFV.select :carrierSolution @00:52:17.60 delay 2 seconds @00:52:19.63 AS.reconfigure ASconfig @00:52:19.68 AS.seek 1ml @00:53:17.47 delay 1 second @00:53:18.49 AFV.select :holdingCoil @00:53:18.59 delay 1 second @00:53:19.62 AS.reconfigure ASfast @00:53:19.67 AS.seek 0ml @00:53:46.97 AFV.select :carrierSolution @00:53:47.08 delay 2 seconds @00:53:49.11 AS.reconfigure ASconfig @00:53:49.16 AS.seek 1ml @00:54:46.94 delay 1 second @00:54:47.96 AFV.select :holdingCoil @00:54:48.06 delay 1 second @00:54:49.09 AS.reconfigure ASfast @00:54:49.14 AS.seek 0ml @00:55:16.47 ARV.dial between carrier and air1 @00:55:16.94 ATV.dial :air3 @00:55:18.17 AS.reconfigure ASslow1 @00:55:18.22 AS.seek 0.01ml @00:55:20.49 delay 2 seconds @00:55:22.51 ATV.dial :bleach @00:55:23.12 AS.seek 0.81ml @00:58:04.23 delay 3 seconds @00:58:07.26 ATV.dial :air3 @00:58:07.80 delay 3 seconds @00:58:10.89 AS.seek 0.835ml @00:58:16.25 delay 3 seconds @00:58:19.28 ATV.dial :bleach @00:58:19.89 AS.seek 0.82ml @00:58:23.16 delay 3 seconds @00:58:26.19 ATV.dial PCR @00:58:26.59 AS.reconfigure ASconfig @00:58:26.64 AS.seek :empty @00:59:14.10 PCR.program :bleachProfile @00:59:14.15 PCR program should complete in < 2:45 @00:59:14.16 PCR.run 10:00 @01:02:43.85 PCR.off @01:02:43.87 AFV.select :carrierSolution @01:02:43.98 delay 2 seconds @01:02:46.02 AS.seek 1ml @01:03:43.86 delay 1 second @01:03:44.88 AFV.select :holdingCoil @01:03:44.98 delay 1 second @01:03:46.01 AS.reconfigure ASfast @01:03:46.06 AS.seek 0ml @01:04:13.39 delay 3 seconds @01:04:16.42 AFV.select :carrierSolution @01:04:16.52 delay 2 seconds @01:04:18.55 AS.reconfigure ASconfig @01:04:18.60 AS.seek 1ml @01:05:16.42 delay 1 second @01:05:17.44 AFV.select :holdingCoil @01:05:17.54 delay 1 second @01:05:18.57 AS.reconfigure ASfast @01:05:18.62 AS.seek 0ml @01:05:45.98 delay 3 seconds @01:05:49.01 AFV.select :carrierSolution @01:05:49.11 delay 2 seconds @01:05:51.14 AS.reconfigure ASconfig @01:05:51.20 AS.seek 1ml @01:06:49.01 delay 1 second @01:06:50.03 AFV.select :holdingCoil @01:06:50.13 delay 1 second @01:06:51.16 AS.reconfigure ASfast @01:06:51.21 AS.seek 0ml @01:07:18.55 delay 3 seconds @01:07:21.58 AFV.select :carrierSolution @01:07:21.68 delay 2 seconds @01:07:23.71 AS.reconfigure ASconfig @01:07:23.77 AS.seek 1ml @01:08:21.53 delay 1 second @01:08:22.55 AFV.select :holdingCoil @01:08:22.65 delay 1 second @01:08:23.68 AS.reconfigure ASfast @01:08:23.73 AS.seek 0ml @01:08:51.06 delay 3 seconds @01:08:54.09 AFV.select :carrierSolution @01:08:54.19 delay 2 seconds @01:08:56.22 AS.reconfigure ASconfig @01:08:56.29 AS.seek 1ml @01:09:54.08 delay 1 second @01:09:55.10 AFV.select :holdingCoil @01:09:55.20 delay 1 second @01:09:56.23 AS.reconfigure ASnorm @01:09:56.28 AS.seek 0ml @01:10:46.98 AFV.select :carrierSolution @01:10:47.09 delay 2 seconds @01:10:49.12 AS.reconfigure ASconfig @01:10:49.17 AS.seek 1ml @01:11:46.97 delay 1 second @01:11:47.99 AFV.select :holdingCoil @01:11:48.09 delay 1 second @01:11:49.12 ATV.dial :waste @01:11:50.28 AS.reconfigure ASnorm @01:11:50.33 AS.seek 0.5ml @01:12:15.89 ATV.dial :waste2 @01:12:17.11 AS.seek 0ml @01:12:42.65 AS.coast @01:12:42.69 SS.coast @01:12:42.73 CC.coast @01:12:42.76 CS.coast @01:12:42.80 PC.coast @01:12:42.85 PS.coast @01:12:42.89 Forearm.coast @01:12:42.93 Elbow.coast @01:12:43.32 Gate.power :core,:OFF @01:12:43.33 Gate.power :raw,:OFF @01:12:43.34 Shutdown @01:12:43.36 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @10:00:24.35
Powering core bus @10:00:29.12 Gate.power :raw,:ON @10:00:29.80 SPE.coast @10:00:29.86 SPE.configure SPEconfig @10:00:29.96 AS.configure ASconfig @10:00:30.07 SS.configure SSconfig @10:00:30.17 CC.configure CCconfig @10:00:30.26 CS.configure CSconfig @10:00:30.36 PC.configure PCconfig @10:00:30.46 PS.configure PSconfig @10:00:30.59 Forearm.configure LooseForearm @10:00:30.69 Elbow.configure LooseElbow @10:00:30.81 SC.configure SCloose @10:00:30.90 SE.configure SEconfig @10:00:30.95 CH.coast @10:00:31.01 CH.configure CHconfig @10:00:31.04 PH.coast @10:00:31.10 PH.configure PHconfig @10:00:31.14 PCR.off @10:00:31.17 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:31.21 PCR.caltemp= 2.8814,0.0372,0 @10:00:31.24 All dwarf controllers running firmware version 3.75 @10:00:31.35 Valve.reset @10:00:31.53 PCR.off @10:00:31.57 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:31.60 PCR.caltemp= 2.8814,0.0372,0 @10:00:31.84 PSV.dial :air @10:00:31.86 Intake.select :close @10:00:31.90 Exhaust.select :close @10:00:31.93 CTV.dial :air @10:00:32.03 CSV.dial :waste1 @10:00:32.14
Forearm.reconfigure LooseForearm @10:00:32.20 SSV.dial :spit,avoiding: :exhaust @10:00:32.22
Forearm.home.seek :retract @10:00:32.36 PS.reconfigure PSconfig @10:00:32.44 PS.home.seek 0ml @10:00:32.98 Hand.close @10:00:33.01
Elbow.jog 1500 @10:00:33.15 CS.reconfigure CSfast @10:00:33.18 SS.reconfigure SSconfig @10:00:33.27 CS.home.seek 0ml @10:00:33.31 SS.home.seek 0ml @10:00:33.83
Elbow.reconfigure LooseElbow @10:00:33.88 Elbow.home.seek :clear @10:00:37.24 SE.reconfigure SEconfig @10:00:37.29 SE.home.seek :down @10:00:37.90 SC.reconfigure SCsloppy @10:00:37.95 SC.home.seek :initial @10:00:40.34 Forearm.seek :retract @10:00:40.41 Elbow.seek :hammer @10:00:41.47 PS.coast @10:00:41.67
Forearm.reconfigure TightForearm @10:00:41.72 Forearm.seek 5 + 350 counts @10:00:42.87 Forearm.seek :retract @10:00:44.09 PC.reconfigure PCconfig @10:00:44.10
CC.reconfigure CCconfig @10:00:44.19 PC.home.seek :open @10:00:44.23
CC.home.seek :open @10:00:47.73 Phase [stdphase] @10:00:47.74 GoodNews.email "At 10:00:47 began in tube 2: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:124\n{1 => 9, 2 => 13}\nCan@10:00:47, 7.3C, 42% humidity, 14.3psia, 14.247V, 0.695A, 0.695A avg, 9.90W",Subject: "Started 20invasivedeployfinal phase stdphase" @10:00:47.78CEST16-May-20
===> STARTED Superphase (phase #3) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @10:00:47.80 Starting initialPurge @10:00:47.82 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:00:47.89 Hand.open @10:00:48.63 Elbow.reconfigure TightElbow @10:00:48.68 Elbow.seek FlushPuck::Garage @10:00:50.65 Elbow.reconfigure LooseElbow @10:00:50.77 Forearm.reconfigure LooseForearm @10:00:50.82 Forearm.seek :garage @10:00:51.83 Hand.close @10:00:52.82 Forearm.seek :retract @10:00:53.86 Elbow.reconfigure TightElbow @10:00:53.98 Elbow.seek CC @10:00:57.21 Elbow.reconfigure LooseElbow @10:00:57.42 Forearm.reconfigure TightForearm @10:00:57.53 Forearm.seek CC @10:00:59.21 CC.seek :closed @10:01:03.75 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.91 @10:01:03.92 Hand.open @10:01:04.80 Forearm.reconfigure LooseForearm @10:01:04.89 Forearm.seek :retract @10:01:06.01 Flushing the Collection Valve::Manifold @10:01:06.32 CSV.dial CSR @10:01:07.85 Pulling 5ml of CSR.flush @10:01:07.87 CSR.select :flush @10:01:08.05 CS.reconfigure CSconfig @10:01:08.19 CS.seek 5ml @10:01:22.10 delay 5 seconds @10:01:27.15 CSR.select :air @10:01:27.42 CS.reconfigure CSfast @10:01:27.51 CS.seek 7.5ml @10:01:34.13 CSV.dial :waste @10:01:35.51 CS.reconfigure CSconfig @10:01:35.61 CS.seek 0ml @10:01:55.81 Purging external Intake @10:01:55.95 CSV.dial :flush @10:01:56.41 CS.reconfigure CSfast @10:01:56.46 CS.seek 10ml @10:02:19.20 delay 5 seconds @10:02:24.23 CSV.dial CBV @10:02:24.27
CBV.dial CSV @10:02:25.16 CTV.dial :intake @10:02:25.94 Intake.select :open @10:02:26.06 CS.seek 0ml @10:02:49.89 Intake.select :close @10:02:50.00 CTV.dial :air @10:02:50.77 Flushing upper manifold @10:02:50.90 CSV.dial :flush @10:02:51.64 CS.seek 5ml @10:03:03.89 delay 5 seconds @10:03:08.91 CSV.dial :air @10:03:09.43 CS.seek 7ml @10:03:14.86 CSV.dial CBV @10:03:14.88
CBV.dial CSV @10:03:15.92 CTV.dial :air @10:03:15.94 CS.seek 0ml @10:03:32.11 delay 7 seconds @10:03:39.15 CS.seek 3ml @10:03:46.64 CSV.dial :waste @10:03:47.11 CS.seek 0ml @10:03:54.76 CSV.dial :flush @10:03:55.23 CS.seek 3ml @10:04:02.71 delay 5 seconds @10:04:07.74 CSV.dial :air @10:04:08.26 CS.seek 5ml @10:04:13.74 CSV.dial :air @10:04:13.76 CS.seek 0ml @10:04:25.64 delay 7 seconds @10:04:32.68 CS.seek 3ml @10:04:40.39 CSV.dial :waste @10:04:41.14 CS.seek 0ml @10:04:48.71 Garaging FlushPuck in Collection Clamp @10:04:48.79 Elbow.reconfigure TightElbow @10:04:48.84 Elbow.seek CC @10:04:49.10 Elbow.reconfigure LooseElbow @10:04:49.21 Forearm.seek CC @10:04:50.21 Hand.close @10:04:50.88 CC.seek :open @10:04:55.24 Forearm.seek :retract @10:04:56.26 Elbow.reconfigure TightElbow @10:04:56.31 Elbow.seek :garage @10:04:59.65 Elbow.reconfigure LooseElbow @10:04:59.75 Forearm.reconfigure TightForearm @10:04:59.80 Forearm.seek :garage @10:05:00.91 Hand.open @10:05:01.58 Forearm.reconfigure LooseForearm @10:05:01.62 Forearm.seek :retract @10:05:02.71 Starting mfb @10:05:02.73 pcrBAC sampling at most 1500ml, wcr at most 1500ml @10:05:02.76 BACfans.email "Started mfb in tube 2 at 10:05:02CEST16-May-20\n{1 => 9, 2 => 13}\nCan@10:04:16, 7.3C, 42% humidity, 14.3psia, 14.247V, 0.651A, 0.651A avg, 9.27W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @10:05:02.87
Loading sh1 puck from tube 2 into Collection Clamp @10:05:02.97 Readying the MFB @10:05:03.03 Homing MFB Syringe @10:05:03.04 AFV.select :holdingCoil @10:05:03.28 ATV.dial :air2 @10:05:03.39
SC.reconfigure SCloose @10:05:03.65 Elbow.reconfigure TightElbow @10:05:03.70 Elbow.seek 2 @10:05:03.87 AS.reconfigure ASconfig @10:05:03.91 AS.home.seek :home @10:05:05.86
Forearm.reconfigure TightForearm @10:05:05.91 Forearm.seek 2 + 700 counts @10:05:06.94 SE.reconfigure SEpick @10:05:07.02 SE.seek beamBreak @10:05:08.92 SE.configure SEconfig @10:05:09.31 Elbow.seek 2 @10:05:09.50 AFV.select :carrierSolution @10:05:09.79 delay 2 seconds @10:05:10.14
Forearm.seek 2 + 300 counts @10:05:10.65 Forearm.reconfigure LooseForearm @10:05:10.80 Hand.close @10:05:11.50 SE.seek :down @10:05:12.04 AS.seek 1ml @10:05:12.24
Forearm.seek :retract @10:05:13.23 SE.coast @10:05:13.43
Elbow.seek CC @10:05:15.42 Elbow.reconfigure LooseElbow @10:05:15.72 Forearm.reconfigure TightForearm @10:05:15.82 Forearm.seek CC @10:05:17.58 CC.seek :closed @10:05:22.00 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.02 @10:05:22.04 Hand.open @10:05:22.83 Forearm.reconfigure LooseForearm @10:05:22.92 Forearm.seek :retract @10:05:23.98 SC.coast @10:05:24.10 collectSample 1500ml w/bubble point of 55psi @10:05:24.13 Priming 7ml @10:05:24.14 Intake.select :close @10:05:24.17 Exhaust.select :close @10:05:24.20 SS.hold @10:05:24.69 Calibrating pressure and temperature sensors @10:05:24.72 CTV.dial :air @10:05:24.75 SSV.dial :spit,avoiding: :exhaust @10:05:24.80 SS.reconfigure SSfast @10:05:24.89 SS.seek 5ml @10:05:27.62 SSV.dial CBV @10:05:27.70
CBV.dial SSV @10:05:29.15 delay 3.8 seconds @10:05:33.24 SSV.dial :spit @10:05:34.64 SS.seek 0ml @10:05:37.31 SSV.dial CBV @10:05:38.71 CTV.dial :intake @10:05:39.58 SSV.dial CBV @10:05:39.64
CBV.dial SSV @10:05:39.67 Allowing 40s for 7.0ml of remaining 7.0ml @10:05:39.69 Intake.select :open @10:05:42.00 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:05:42.03 SS.reconfigure SSinhale.with maxSpeed: 150 @10:05:42.13 SS.seek 7ml @10:05:45.58 Vacuum too high -- waiting up to 40s @10:05:48.39 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:05:48.45 SS.reconfigure SSinhale.with maxSpeed: 135 @10:05:48.55 SS.seek 7ml @10:05:51.80 Vacuum too high -- waiting up to 40s @10:05:54.36 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:05:54.39 SS.reconfigure SSinhale.with maxSpeed: 122 @10:05:54.49 SS.seek 7ml @10:05:54.85 Stroke completed -- waiting for pressure to equalize ... @10:05:58.08 Intake.select :close @10:05:58.32 SSV.dial :exhaust @10:05:59.17 Exhaust.select :open @10:05:59.34 SS.reconfigure SSexhale @10:05:59.43 SS.seek 0ml @10:06:03.30 Exhaust.select :close @10:06:03.47 Sampling 1500.0ml @10:06:03.49 Intake.select :close @10:06:03.52 Exhaust.select :close @10:06:03.54 SS.reconfigure SSconfig @10:06:03.64 SS.hold @10:06:04.07 CTV.dial :intake @10:06:04.10 SSV.dial CBV @10:06:04.19
CBV.dial SSV @10:06:04.99 delay 1 second @10:06:05.09
Allowing 130s for 25.0ml of remaining 1500.0ml @10:06:05.11 Intake.select :open @10:06:06.04 AFV.select :holdingCoil @10:06:06.17 delay 1 second @10:06:07.20 ATV.dial :waste @10:06:07.41
Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:06:07.44 SS.reconfigure SSinhale.with maxSpeed: 122 @10:06:07.55 SS.seek 25ml @10:06:08.30 AS.reconfigure ASnorm @10:06:08.40 AS.home.seek :empty @10:06:11.01
Vacuum too high -- waiting up to 40s @10:06:13.10 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:06:13.11 SS.reconfigure SSinhale.with maxSpeed: 110 @10:06:13.16 SS.seek 25ml @10:06:15.86 Vacuum too high -- waiting up to 40s @10:06:17.96 SS.reconfigure SSinhale.with maxSpeed: 99 @10:06:18.01 SS.seek 25ml @10:06:20.48 Vacuum too high -- waiting up to 40s @10:06:23.11 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:06:23.12 SS.reconfigure SSinhale.with maxSpeed: 89 @10:06:23.17 SS.seek 25ml @10:06:26.36 Vacuum too high -- waiting up to 40s @10:06:28.20 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:06:28.22 SS.reconfigure SSinhale.with maxSpeed: 80 @10:06:28.27 SS.seek 25ml @10:06:31.54 Vacuum too high -- waiting up to 40s @10:06:33.39 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:06:33.40 SS.reconfigure SSinhale.with maxSpeed: 72 @10:06:33.45 SS.seek 25ml @10:06:36.14 Vacuum too high -- waiting up to 40s @10:06:37.97 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:06:37.99 SS.reconfigure SSinhale.with maxSpeed: 65 @10:06:38.04 SS.seek 25ml @10:06:41.45 Vacuum too high -- waiting up to 40s @10:06:43.28 SS.reconfigure SSinhale.with maxSpeed: 59 @10:06:43.33 SS.seek 25ml @10:06:47.08 Vacuum too high -- waiting up to 40s @10:06:48.90 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:06:48.92 SS.reconfigure SSinhale.with maxSpeed: 53 @10:06:48.97 SS.seek 25ml @10:06:53.45 Stroke completed -- waiting for pressure to equalize ... @10:06:59.06 Intake.select :close @10:06:59.22 SSV.dial :exhaust @10:07:00.01 Exhaust.select :open @10:07:00.13 SS.reconfigure SSexhale @10:07:00.17 SS.seek 0ml @10:07:01.00 ATV.dial :waste @10:07:01.01 ARV.dial between carrier and air1 @10:07:01.10 ASV.dial :waste1 @10:07:09.18
Exhaust.select :close @10:07:09.31 SSV.dial CBV @10:07:09.33
CBV.dial SSV @10:07:10.15 Allowing 130s for 25.0ml of remaining 1475.0ml @10:07:10.16 Intake.select :open @10:07:12.34 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:07:12.35 SS.reconfigure SSinhale.with maxSpeed: 53 @10:07:12.40 SS.seek 25ml @10:07:17.36 Vacuum too high -- waiting up to 40s @10:07:18.92 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:07:18.94 SS.reconfigure SSinhale.with maxSpeed: 48 @10:07:18.99 SS.seek 25ml @10:07:24.23 Vacuum too high -- waiting up to 40s @10:07:25.80 SS.reconfigure SSinhale.with maxSpeed: 43 @10:07:25.85 SS.seek 25ml @10:07:32.39 Vacuum too high -- waiting up to 40s @10:07:33.95 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:07:33.97 SS.reconfigure SSinhale.with maxSpeed: 39 @10:07:34.02 SS.seek 25ml @10:07:41.53 Vacuum too high -- waiting up to 40s @10:07:43.10 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:07:43.11 SS.reconfigure SSinhale.with maxSpeed: 35 @10:07:43.16 SS.seek 25ml @10:07:55.48 Vacuum too high -- waiting up to 40s @10:07:57.04 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:07:57.06 SS.reconfigure SSinhale.with maxSpeed: 32 @10:07:57.11 SS.seek 25ml @10:08:07.41 Stroke completed -- waiting for pressure to equalize ... @10:08:12.42 Intake.select :close @10:08:12.57 SSV.dial :exhaust @10:08:13.37 Exhaust.select :open @10:08:13.49 SS.reconfigure SSexhale @10:08:13.53 SS.seek 0ml @10:08:22.54 Exhaust.select :close @10:08:22.65 SSV.dial CBV @10:08:22.69
CBV.dial SSV @10:08:23.49 Allowing 130s for 25.0ml of remaining 1450.0ml @10:08:23.50 Intake.select :open @10:08:25.67 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:08:25.69 SS.reconfigure SSinhale.with maxSpeed: 32 @10:08:25.74 SS.seek 25ml @10:08:47.33 Vacuum too high -- waiting up to 40s @10:08:48.89 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:08:48.91 SS.reconfigure SSinhale.with maxSpeed: 29 @10:08:48.96 SS.seek 25ml @10:09:25.70 Stroke completed -- waiting for pressure to equalize ... @10:09:30.78 Intake.select :close @10:09:30.94 SSV.dial :exhaust @10:09:31.73 Exhaust.select :open @10:09:31.85 SS.reconfigure SSexhale @10:09:31.89 SS.seek 0ml @10:09:40.74 Exhaust.select :close @10:09:40.86 SSV.dial CBV @10:09:40.88
CBV.dial SSV @10:09:41.70 Allowing 130s for 25.0ml of remaining 1425.0ml @10:09:41.71 Intake.select :open @10:09:43.88 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:09:43.90 SS.reconfigure SSinhale.with maxSpeed: 29 @10:09:43.95 SS.seek 25ml @10:10:44.30 Stroke completed -- waiting for pressure to equalize ... @10:10:49.36 Intake.select :close @10:10:49.52 SSV.dial :exhaust @10:10:50.31 Exhaust.select :open @10:10:50.43 SS.reconfigure SSexhale @10:10:50.48 SS.seek 0ml @10:10:59.43 Exhaust.select :close @10:10:59.55 SSV.dial CBV @10:10:59.57
CBV.dial SSV @10:11:00.38 Allowing 130s for 25.0ml of remaining 1400.0ml @10:11:00.39 Intake.select :open @10:11:02.56 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:11:02.58 SS.reconfigure SSinhale.with maxSpeed: 29 @10:11:02.63 SS.seek 25ml @10:12:03.02 Stroke completed -- waiting for pressure to equalize ... @10:12:08.61 Intake.select :close @10:12:08.77 SSV.dial :exhaust @10:12:09.56 Exhaust.select :open @10:12:09.68 SS.reconfigure SSexhale @10:12:09.72 SS.seek 0ml @10:12:18.65 Exhaust.select :close @10:12:18.77 SSV.dial CBV @10:12:18.79
CBV.dial SSV @10:12:19.60 Allowing 130s for 25.0ml of remaining 1375.0ml @10:12:19.61 Intake.select :open @10:12:21.79 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:12:21.80 SS.reconfigure SSinhale.with maxSpeed: 29 @10:12:21.85 SS.seek 25ml @10:13:22.31 Stroke completed -- waiting for pressure to equalize ... @10:13:27.37 Intake.select :close @10:13:27.53 SSV.dial :exhaust @10:13:28.32 Exhaust.select :open @10:13:28.44 SS.reconfigure SSexhale @10:13:28.49 SS.seek 0ml @10:13:37.42 Exhaust.select :close @10:13:37.54 SSV.dial CBV @10:13:37.56
CBV.dial SSV @10:13:38.39 Allowing 130s for 25.0ml of remaining 1350.0ml @10:13:38.40 Intake.select :open @10:13:40.57 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:13:40.59 SS.reconfigure SSinhale.with maxSpeed: 29 @10:13:40.64 SS.seek 25ml @10:14:40.98 Stroke completed -- waiting for pressure to equalize ... @10:14:46.66 Intake.select :close @10:14:46.81 SSV.dial :exhaust @10:14:47.60 Exhaust.select :open @10:14:47.72 SS.reconfigure SSexhale @10:14:47.76 SS.seek 0ml @10:14:56.51 Exhaust.select :close @10:14:56.63 SSV.dial CBV @10:14:56.65
CBV.dial SSV @10:14:57.46 Allowing 130s for 25.0ml of remaining 1325.0ml @10:14:57.47 Intake.select :open @10:14:59.64 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:14:59.66 SS.reconfigure SSinhale.with maxSpeed: 29 @10:14:59.71 SS.seek 25ml @10:16:00.40 Stroke completed -- waiting for pressure to equalize ... @10:16:06.05 Intake.select :close @10:16:06.73 SSV.dial :exhaust @10:16:07.51 Exhaust.select :open @10:16:07.63 SS.reconfigure SSexhale @10:16:07.67 SS.seek 0ml @10:16:16.42 Exhaust.select :close @10:16:16.54 SSV.dial CBV @10:16:16.56
CBV.dial SSV @10:16:17.39 Allowing 130s for 25.0ml of remaining 1300.0ml @10:16:17.40 Intake.select :open @10:16:19.58 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:16:19.60 SS.reconfigure SSinhale.with maxSpeed: 29 @10:16:19.65 SS.seek 25ml @10:17:20.36 Stroke completed -- waiting for pressure to equalize ... @10:17:26.08 Intake.select :close @10:17:26.24 SSV.dial :exhaust @10:17:27.03 Exhaust.select :open @10:17:27.15 SS.reconfigure SSexhale @10:17:27.19 SS.seek 0ml @10:17:35.95 Exhaust.select :close @10:17:36.07 SSV.dial CBV @10:17:36.09
CBV.dial SSV @10:17:36.90 Allowing 130s for 25.0ml of remaining 1275.0ml @10:17:36.91 Intake.select :open @10:17:39.08 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:17:39.10 SS.reconfigure SSinhale.with maxSpeed: 29 @10:17:39.15 SS.seek 25ml @10:18:39.69 Stroke completed -- waiting for pressure to equalize ... @10:18:46.01 Intake.select :close @10:18:46.17 SSV.dial :exhaust @10:18:46.95 Exhaust.select :open @10:18:47.07 SS.reconfigure SSexhale @10:18:47.12 SS.seek 0ml @10:18:56.26 Exhaust.select :close @10:18:56.38 SSV.dial CBV @10:18:56.40
CBV.dial SSV @10:18:57.22 Allowing 130s for 25.0ml of remaining 1250.0ml @10:18:57.23 Intake.select :open @10:18:59.40 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:18:59.42 SS.reconfigure SSinhale.with maxSpeed: 29 @10:18:59.47 SS.seek 25ml @10:19:55.73 Vacuum too high -- waiting up to 40s @10:19:57.55 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:19:57.57 SS.reconfigure SSinhale.with maxSpeed: 26 @10:19:57.62 SS.seek 25ml @10:20:02.71 Stroke completed -- waiting for pressure to equalize ... @10:20:07.73 Intake.select :close @10:20:07.89 SSV.dial :exhaust @10:20:08.68 Exhaust.select :open @10:20:08.80 SS.reconfigure SSexhale @10:20:08.84 SS.seek 0ml @10:20:17.87 Exhaust.select :close @10:20:17.99 SSV.dial CBV @10:20:18.01
CBV.dial SSV @10:20:18.81 Allowing 130s for 25.0ml of remaining 1225.0ml @10:20:18.82 Intake.select :open @10:20:21.00 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:20:21.01 SS.reconfigure SSinhale.with maxSpeed: 26 @10:20:21.07 SS.seek 25ml @10:21:28.43 Stroke completed -- waiting for pressure to equalize ... @10:21:34.14 Intake.select :close @10:21:34.29 SSV.dial :exhaust @10:21:35.06 Exhaust.select :open @10:21:35.18 SS.reconfigure SSexhale @10:21:35.23 SS.seek 0ml @10:21:44.04 Exhaust.select :close @10:21:44.16 SSV.dial CBV @10:21:44.18
CBV.dial SSV @10:21:44.98 Allowing 130s for 25.0ml of remaining 1200.0ml @10:21:44.99 Intake.select :open @10:21:47.16 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:21:47.18 SS.reconfigure SSinhale.with maxSpeed: 26 @10:21:47.23 SS.seek 25ml @10:22:54.83 Stroke completed -- waiting for pressure to equalize ... @10:23:01.13 Intake.select :close @10:23:01.28 SSV.dial :exhaust @10:23:02.04 Exhaust.select :open @10:23:02.16 SS.reconfigure SSexhale @10:23:02.21 SS.seek 0ml @10:23:11.00 Exhaust.select :close @10:23:11.12 SSV.dial CBV @10:23:11.14
CBV.dial SSV @10:23:11.94 Allowing 130s for 25.0ml of remaining 1175.0ml @10:23:11.95 Intake.select :open @10:23:14.12 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:23:14.14 SS.reconfigure SSinhale.with maxSpeed: 26 @10:23:14.19 SS.seek 25ml @10:24:20.70 Vacuum too high -- waiting up to 40s @10:24:22.53 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:24:22.99 SS.reconfigure SSinhale.with maxSpeed: 23 @10:24:23.13 SS.seek 25ml @10:24:24.88 Stroke completed -- waiting for pressure to equalize ... @10:24:29.37 Intake.select :close @10:24:29.53 SSV.dial :exhaust @10:24:30.31 Exhaust.select :open @10:24:30.43 SS.reconfigure SSexhale @10:24:30.47 SS.seek 0ml @10:24:39.40 Exhaust.select :close @10:24:39.52 SSV.dial CBV @10:24:39.54
CBV.dial SSV @10:24:40.34 Allowing 130s for 25.0ml of remaining 1150.0ml @10:24:40.35 Intake.select :open @10:24:42.53 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:24:42.54 SS.reconfigure SSinhale.with maxSpeed: 23 @10:24:42.59 SS.seek 25ml @10:25:58.91 Stroke completed -- waiting for pressure to equalize ... @10:26:05.21 Intake.select :close @10:26:05.36 SSV.dial :exhaust @10:26:06.14 Exhaust.select :open @10:26:06.26 SS.reconfigure SSexhale @10:26:06.30 SS.seek 0ml @10:26:15.30 Exhaust.select :close @10:26:15.42 SSV.dial CBV @10:26:15.44
CBV.dial SSV @10:26:16.26 Allowing 130s for 25.0ml of remaining 1125.0ml @10:26:16.27 Intake.select :open @10:26:18.44 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:26:18.46 SS.reconfigure SSinhale.with maxSpeed: 23 @10:26:18.50 SS.seek 25ml @10:27:34.58 Stroke completed -- waiting for pressure to equalize ... @10:27:42.14 Intake.select :close @10:27:42.29 SSV.dial :exhaust @10:27:43.08 Exhaust.select :open @10:27:43.20 SS.reconfigure SSexhale @10:27:43.25 SS.seek 0ml @10:27:52.36 Exhaust.select :close @10:27:52.48 SSV.dial CBV @10:27:52.50
CBV.dial SSV @10:27:53.31 Allowing 130s for 25.0ml of remaining 1100.0ml @10:27:53.32 Intake.select :open @10:27:55.49 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:27:55.51 SS.reconfigure SSinhale.with maxSpeed: 23 @10:27:55.56 SS.seek 25ml @10:29:07.57 Vacuum too high -- waiting up to 40s @10:29:09.68 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:29:09.70 SS.reconfigure SSinhale.with maxSpeed: 21 @10:29:09.75 SS.seek 25ml @10:29:15.07 Stroke completed -- waiting for pressure to equalize ... @10:29:21.42 Intake.select :close @10:29:21.58 SSV.dial :exhaust @10:29:22.37 Exhaust.select :open @10:29:22.49 SS.reconfigure SSexhale @10:29:22.53 SS.seek 0ml @10:29:31.59 Exhaust.select :close @10:29:31.71 SSV.dial CBV @10:29:31.73
CBV.dial SSV @10:29:32.56 Allowing 130s for 25.0ml of remaining 1075.0ml @10:29:32.57 Intake.select :open @10:29:34.74 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:29:34.76 SS.reconfigure SSinhale.with maxSpeed: 21 @10:29:34.81 SS.seek 25ml @10:30:58.12 Stroke completed -- waiting for pressure to equalize ... @10:31:05.67 Intake.select :close @10:31:05.82 SSV.dial :exhaust @10:31:06.61 Exhaust.select :open @10:31:06.73 SS.reconfigure SSexhale @10:31:06.78 SS.seek 0ml @10:31:15.92 Exhaust.select :close @10:31:16.04 SSV.dial CBV @10:31:16.06
CBV.dial SSV @10:31:16.86 Allowing 130s for 25.0ml of remaining 1050.0ml @10:31:16.87 Intake.select :open @10:31:19.04 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:31:19.06 SS.reconfigure SSinhale.with maxSpeed: 21 @10:31:19.11 SS.seek 25ml @10:32:38.24 Vacuum too high -- waiting up to 40s @10:32:40.30 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:32:40.32 SS.reconfigure SSinhale.with maxSpeed: 19 @10:32:40.37 SS.seek 25ml @10:32:46.08 Stroke completed -- waiting for pressure to equalize ... @10:32:52.43 Intake.select :close @10:32:52.59 SSV.dial :exhaust @10:32:53.36 Exhaust.select :open @10:32:53.48 SS.reconfigure SSexhale @10:32:53.52 SS.seek 0ml @10:33:02.55 Exhaust.select :close @10:33:02.67 SSV.dial CBV @10:33:02.69
CBV.dial SSV @10:33:03.50 Allowing 130s for 25.0ml of remaining 1025.0ml @10:33:03.51 Intake.select :open @10:33:05.68 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:33:05.70 SS.reconfigure SSinhale.with maxSpeed: 19 @10:33:05.75 SS.seek 25ml @10:34:38.02 Stroke completed -- waiting for pressure to equalize ... @10:34:46.79 Intake.select :close @10:34:46.95 SSV.dial :exhaust @10:34:47.74 Exhaust.select :open @10:34:47.86 SS.reconfigure SSexhale @10:34:47.91 SS.seek 0ml @10:34:56.74 Exhaust.select :close @10:34:56.86 SSV.dial CBV @10:34:56.89
CBV.dial SSV @10:34:57.71 Allowing 130s for 25.0ml of remaining 1000.0ml @10:34:57.72 Intake.select :open @10:34:59.89 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:34:59.91 SS.reconfigure SSinhale.with maxSpeed: 19 @10:34:59.96 SS.seek 25ml @10:36:32.68 Stroke completed -- waiting for pressure to equalize ... @10:36:43.25 Intake.select :close @10:36:43.41 SSV.dial :exhaust @10:36:44.21 Exhaust.select :open @10:36:44.33 SS.reconfigure SSexhale @10:36:44.37 SS.seek 0ml @10:36:53.42 Exhaust.select :close @10:36:53.53 SSV.dial CBV @10:36:53.57
CBV.dial SSV @10:36:54.38 Allowing 130s for 25.0ml of remaining 975.0ml @10:36:54.39 Intake.select :open @10:36:56.56 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:36:56.58 SS.reconfigure SSinhale.with maxSpeed: 19 @10:36:56.63 SS.seek 25ml @10:37:44.56 Vacuum too high -- waiting up to 40s @10:37:46.94 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:37:46.97 SS.reconfigure SSinhale.with maxSpeed: 17 @10:37:47.04 SS.seek 25ml @10:38:36.87 Stroke completed -- waiting for pressure to equalize ... @10:38:46.92 Intake.select :close @10:38:47.08 SSV.dial :exhaust @10:38:47.86 Exhaust.select :open @10:38:47.98 SS.reconfigure SSexhale @10:38:48.03 SS.seek 0ml @10:38:57.14 Exhaust.select :close @10:38:57.26 SSV.dial CBV @10:38:57.28
CBV.dial SSV @10:38:58.11 Allowing 130s for 25.0ml of remaining 950.0ml @10:38:58.12 Intake.select :open @10:39:00.29 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:39:00.31 SS.reconfigure SSinhale.with maxSpeed: 17 @10:39:00.36 SS.seek 25ml @10:40:30.83 Vacuum too high -- waiting up to 40s @10:40:33.40 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:40:33.42 SS.reconfigure SSinhale.with maxSpeed: 15 @10:40:34.01 SS.seek 25ml @10:40:48.74 Stroke completed -- waiting for pressure to equalize ... @10:40:58.20 Intake.select :close @10:40:58.35 SSV.dial :exhaust @10:40:59.15 Exhaust.select :open @10:40:59.27 SS.reconfigure SSexhale @10:40:59.32 SS.seek 0ml @10:41:08.52 Exhaust.select :close @10:41:08.64 SSV.dial CBV @10:41:08.66
CBV.dial SSV @10:41:09.47 Allowing 130s for 25.0ml of remaining 925.0ml @10:41:09.48 Intake.select :open @10:41:11.65 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:41:11.67 SS.reconfigure SSinhale.with maxSpeed: 15 @10:41:11.72 SS.seek 25ml @10:43:08.70 Stroke completed -- waiting for pressure to equalize ... @10:43:19.36 Intake.select :close @10:43:19.52 SSV.dial :exhaust @10:43:20.30 Exhaust.select :open @10:43:20.42 SS.reconfigure SSexhale @10:43:20.47 SS.seek 0ml @10:43:29.55 Exhaust.select :close @10:43:29.67 SSV.dial CBV @10:43:29.69
CBV.dial SSV @10:43:30.49 Allowing 130s for 25.0ml of remaining 900.0ml @10:43:30.50 Intake.select :open @10:43:32.67 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:43:32.69 SS.reconfigure SSinhale.with maxSpeed: 15 @10:43:32.74 SS.seek 25ml @10:45:26.56 Vacuum too high -- waiting up to 40s @10:45:29.42 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:45:29.44 SS.reconfigure SSinhale.with maxSpeed: 14 @10:45:29.49 SS.seek 25ml @10:45:33.15 Stroke completed -- waiting for pressure to equalize ... @10:45:40.51 SS.reconfigure SSconfig @10:45:40.56 SS.hold @10:45:40.69 Intake.select :close @10:45:40.85 ShallowSampler::Clogged in MAIN -- progress too slow after 625.0ml @10:45:40.86 SSV.dial :exhaust @10:45:41.65 Exhaust.select :open @10:45:41.77 SS.reconfigure SSexhale @10:45:41.81 SS.seek 0ml @10:45:50.77 Exhaust.select :close @10:45:50.88 Sampled 625.0ml @10:45:50.90 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:45:50.91 Intake.select :close @10:45:50.98 CSV.dial :waste @10:45:51.02 CS.seek 0ml @10:45:51.14 CSV.dial :air @10:45:51.17 SSV.dial CBV,avoiding: :exhaust @10:45:51.22
CBV.dial SSV @10:45:51.99 CS.seek 10ml @10:45:53.15
CTV.dial :air @10:45:53.93 delay 15 seconds @10:46:08.96 SSV.dial :spit,avoiding: :exhaust @10:46:10.33 CTV.dial CSV,avoiding: :intake @10:46:10.37
SS.seek 0ml @10:46:10.51 SSV.dial CBV,avoiding: :exhaust @10:46:15.29 CBV.dial between SSV and waste2 @10:46:15.71 CTV.dial CSV,avoiding: :air @10:46:15.74
CSV.dial CTV @10:46:16.19 CS.reconfigure CSpressurize @10:46:16.23 CS.seek 0ml @10:46:41.90 BubblePoint: Intake @69.5psia, Outlet @13.8psia @10:46:41.91 CBV.dial SSV @10:46:42.26 SS.reconfigure SSconfig @10:46:42.31 SS.seek 10ml @10:46:47.68 delay 15 seconds @10:47:02.81 Evacuated: Intake @35.7psia, Outlet @0.1psia @10:47:02.83 SSV.dial :spit,avoiding: :exhaust @10:47:04.18 SS.reconfigure SSexhale @10:47:04.23 SS.seek 0ml @10:47:09.19 SS.reconfigure SSfast @10:47:09.24 SS.seek 5ml @10:47:11.91 SSV.dial CBV,avoiding: :exhaust @10:47:11.94
CBV.dial SSV @10:47:13.30 CTV.dial :air,avoiding: :intake @10:47:14.65 CSV.dial CTV @10:47:14.69
SS.seek 10ml @10:47:15.69 CSV.dial :air @10:47:16.68 CS.reconfigure CSfast @10:47:16.73 CS.seek 0ml @10:47:17.15
SSV.dial :spit,avoiding: :exhaust @10:47:18.17 CS.coast @10:47:18.52
SS.reconfigure SSexhale @10:47:18.56 SS.seek 0ml @10:47:23.42 SS.reconfigure SSfast @10:47:23.47 SS.seek 5ml @10:47:26.14 SSV.dial CBV,avoiding: :exhaust @10:47:26.16
CBV.dial SSV @10:47:28.52 SSV.dial :spit,avoiding: :exhaust @10:47:29.85 SS.reconfigure SSconfig @10:47:29.90 SS.seek 0ml @10:47:32.97 SS.coast @10:47:33.01 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:47:33.02 Intake.select :close @10:47:33.10 CSV.dial :waste @10:47:33.24 SSV.dial CBV,avoiding: :exhaust @10:47:33.26
CBV.dial SSV @10:47:33.92 CS.seek 0ml @10:47:33.99 CSV.dial :air @10:47:34.62
CTV.dial :air @10:47:34.64 delay 15 seconds @10:47:34.74 CS.seek 10ml @10:47:49.66
SSV.dial :spit,avoiding: :exhaust @10:47:51.02 CTV.dial CSV,avoiding: :intake @10:47:51.06
SS.reconfigure SSexhale @10:47:51.16 SS.seek 0ml @10:47:51.22 SSV.dial CBV,avoiding: :exhaust @10:47:57.68 CBV.dial between SSV and waste2 @10:47:58.10 CSV.dial between air and CTV @10:47:58.43 CS.seek 8.233ml @10:48:03.44 CTV.dial CSV,avoiding: :air @10:48:03.46
CSV.dial CTV @10:48:03.79 CS.reconfigure CSpressurize @10:48:03.83 CS.seek 0ml @10:48:25.45 CSV.dial :air @10:48:25.90 CS.reconfigure CSfast @10:48:25.95 CS.seek 10ml @10:48:48.61 CSV.dial between air and CTV @10:48:48.95 CS.seek 3.032ml @10:49:05.36 CTV.dial CSV,avoiding: :air @10:49:05.39
CSV.dial CTV @10:49:05.73 CS.reconfigure CSpressurize @10:49:05.77 CS.seek 0ml @10:49:12.94 BubblePoint: Intake @68.7psia, Outlet @13.1psia @10:49:12.95 CBV.dial SSV @10:49:13.31 SS.reconfigure SSconfig @10:49:13.37 SS.seek 10ml @10:49:18.76 delay 15 seconds @10:49:33.89 Evacuated: Intake @38.5psia, Outlet @15.0psia @10:49:33.91 SSV.dial :spit,avoiding: :exhaust @10:49:35.24 SS.reconfigure SSexhale @10:49:35.29 SS.seek 0ml @10:49:40.16 SS.reconfigure SSfast @10:49:40.21 SS.seek 5ml @10:49:42.88 SSV.dial CBV,avoiding: :exhaust @10:49:42.91
CBV.dial SSV @10:49:44.75 CTV.dial :air,avoiding: :intake @10:49:46.09 CSV.dial CTV @10:49:46.14
SS.seek 10ml @10:49:47.14 CSV.dial :air @10:49:47.61 CS.reconfigure CSfast @10:49:47.66 CS.seek 0ml @10:49:48.52
SSV.dial :spit,avoiding: :exhaust @10:49:49.84 CS.coast @10:49:49.91
SS.reconfigure SSexhale @10:49:49.96 SS.seek 0ml @10:49:54.88 SS.reconfigure SSfast @10:49:54.94 SS.seek 5ml @10:49:57.44 SSV.dial CBV,avoiding: :exhaust @10:49:57.46
CBV.dial SSV @10:49:59.83 SSV.dial :spit,avoiding: :exhaust @10:50:01.18 SS.reconfigure SSconfig @10:50:01.24 SS.seek 0ml @10:50:04.37 SS.coast @10:50:04.41 Lysing: Adding 1.5ml lysis @10:50:04.42 CBV.dial between CSV and waste1,avoiding: :DBV @10:50:05.95 CTV.dial CSV @10:50:07.28 CSV.dial CSR @10:50:08.08 Pulling 1ml of CSR.lysis @10:50:08.09 CSR.select :lysis @10:50:08.21 CS.reconfigure CSconfig @10:50:08.26 CS.seek 1ml @10:50:11.74 delay 5 seconds @10:50:16.77 CSR.select :air @10:50:16.94 CS.seek 6ml @10:50:30.64 delay 2 seconds @10:50:32.66 CSV.dial :waste1 @10:50:33.96 CS.reconfigure CSfast @10:50:34.01 CS.seek :empty @10:50:48.02 CSV.dial CSR @10:50:49.37 Pulling 1.5ml of CSR.lysis @10:50:49.38 CSR.select :lysis @10:50:49.50 CS.reconfigure CSconfig @10:50:49.55 CS.seek 1.5ml @10:50:54.46 delay 5 seconds @10:50:59.49 CSR.select :air @10:50:59.67 CS.seek 4.5ml @10:51:08.36 delay 2 seconds @10:51:10.38 CSV.dial CTV @10:51:10.86 CS.seek 1.275ml @10:51:20.09 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @10:51:20.11 CH.seek 85C,8:00 @10:54:21.49 CH.hold 5:00,85C @10:59:21.65 Collection Puck Heater at 85C after lysing @10:59:21.67 CS.seek 0ml @10:59:25.90 CTV.dial between spare and air,avoiding: :intake @10:59:26.98 CSV.dial :air @10:59:27.46 CS.seek 5ml @10:59:41.19 CSV.dial CTV @10:59:41.65 delay 2 seconds @10:59:43.67 CSV.dial :air @10:59:44.12 delay 2 seconds @10:59:46.14 CSV.dial CTV @10:59:46.61 delay 2 seconds @10:59:48.63 CSV.dial :air @10:59:49.11 delay 2 seconds @10:59:51.14 CS.seek 0ml @11:00:04.90 Recovering Lysate into Collection syringe @11:00:04.91 CSV.dial CBV @11:00:05.94 CBV.dial CSV @11:00:06.37 CS.seek 5ml @11:00:20.12 delay 5 seconds @11:00:25.14 CTV.dial :air @11:00:25.69 CS.seek 7ml @11:00:31.75 delay 5 seconds @11:00:36.77 CSV.dial :air2 @11:00:37.23 CS.seek 10ml @11:00:45.81 CSV.dial between air and CTV,avoiding: CBV @11:00:47.09 Lysing Complete @11:00:47.17 Storing sh1 puck in Collection Clamp to tube 1 @11:00:47.36 SC.reconfigure SCtight @11:00:47.41 SC.seek 1 @11:00:49.52 SC.reconfigure SCloose @11:00:49.74 Elbow.reconfigure TightElbow @11:00:49.79 Elbow.seek 1 @11:00:51.80 Forearm.reconfigure TightForearm @11:00:51.85 Forearm.seek 1 + 700 counts @11:00:52.90 SE.reconfigure SEpick @11:00:52.97 SE.seek beamBreak @11:00:55.19 SE.configure SEconfig @11:00:55.35 Elbow.reconfigure LimpElbow @11:00:55.41 Forearm.reconfigure LooseForearm @11:00:55.45 Forearm.seek 1 @11:00:55.75 Elbow.hold @11:00:55.82 Elbow.reconfigure TightElbow @11:00:55.93 SE.seek 8.09 Pucks below Top Plate @11:00:56.92 Forearm.force -20 @11:00:56.96 Forearm.seek 1 + 324 counts @11:00:57.28 Forearm.reconfigure TightForearm @11:00:57.33 Forearm.seek 1 + 700 counts @11:00:57.64 SE.seek 9.09 Pucks below Top Plate @11:00:58.30 Forearm.reconfigure LooseForearm @11:00:58.35 Forearm.seek :retract @11:00:59.30 Elbow.seek CC @11:01:01.00 Elbow.reconfigure LooseElbow @11:01:01.12 Forearm.seek CC @11:01:02.20 Hand.close @11:01:02.86 CC.seek :open @11:01:07.35 Forearm.seek :retract @11:01:08.38 Elbow.reconfigure TightElbow @11:01:08.43 Elbow.seek 1 @11:01:10.38 Elbow.reconfigure LimpElbow @11:01:10.44 Forearm.seek 1 @11:01:11.44 Elbow.hold @11:01:11.51 Elbow.reconfigure TightElbow @11:01:11.56 Hand.open @11:01:12.38 Forearm.force -20 @11:01:12.42 Forearm.seek 1 + 348 counts @11:01:12.74 Forearm.reconfigure TightForearm @11:01:12.79 Forearm.seek 1 + 700 counts @11:01:13.16 SE.seek :down @11:01:14.32
Forearm.reconfigure LooseForearm @11:01:14.37 Forearm.seek :retract @11:01:15.03 SE.coast @11:01:15.30
SC.coast @11:01:15.34 Tranferring lysate to PRV @11:01:15.35 CSV.dial :air @11:01:15.70 CS.seek 10ml @11:01:15.77 CSV.dial between air and CTV @11:01:16.11 AIV.dial :bypass @11:01:16.20 Beginning samplePassC @11:01:16.21 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:01:16.29 Hand.open @11:01:16.62 Elbow.seek FlushPuck::Garage @11:01:18.67 Elbow.reconfigure LooseElbow @11:01:18.78 Forearm.seek :garage @11:01:19.78 Hand.close @11:01:20.49 Forearm.seek :retract @11:01:21.53 Elbow.reconfigure TightElbow @11:01:21.58 Elbow.seek CC @11:01:25.02 Elbow.reconfigure LooseElbow @11:01:25.13 Forearm.reconfigure TightForearm @11:01:25.18 Forearm.seek CC @11:01:26.52 CC.seek :closed @11:01:30.78 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.86 @11:01:30.86 Hand.open @11:01:31.56 Forearm.reconfigure LooseForearm @11:01:31.61 Forearm.seek :retract @11:01:32.61 AWV.select :air @11:01:32.62 AIV.dial :bypass @11:01:32.63 PSV.dial PSR @11:01:34.01 Pulling 1.5ml of PSR.flush @11:01:34.02 PSR.select :flush @11:01:34.20 PS.seek 1.5ml @11:01:50.23 delay 5 seconds @11:01:55.26 PSV.dial :waste1 @11:01:55.74 PS.seek 0ml @11:02:11.82 PSV.dial PSR @11:02:12.27 PSR.select :air @11:02:12.39 PS.seek 2.5ml @11:02:38.60 PRV.dial CBV @11:02:38.63
CBV.dial PRV @11:02:39.47 PSV.dial :mixloop @11:02:40.49 CTV.dial :air,avoiding: :intake @11:02:40.50 PTV.dial :PRVmixing @11:02:40.60 PS.seek 0ml @11:03:06.67 PSV.dial :air @11:03:07.15 PS.seek 2.5ml @11:03:33.26 PSV.dial :mixloop @11:03:33.72 PS.seek 0ml @11:03:59.80 PSV.dial :air @11:04:00.29 PS.seek 2.5ml @11:04:26.39 PSV.dial :mixloop @11:04:26.87 PS.seek 0ml @11:04:52.95 Garaging FlushPuck in Collection Clamp @11:04:53.03 Elbow.reconfigure TightElbow @11:04:53.08 Elbow.seek CC @11:04:53.35 Elbow.reconfigure LooseElbow @11:04:53.46 Forearm.seek CC @11:04:54.49 Hand.close @11:04:55.12 CC.seek :open @11:04:59.48 Forearm.seek :retract @11:05:00.49 Elbow.reconfigure TightElbow @11:05:00.54 Elbow.seek :garage @11:05:03.74 Elbow.reconfigure LooseElbow @11:05:03.84 Forearm.reconfigure TightForearm @11:05:03.89 Forearm.seek :garage @11:05:05.04 Hand.open @11:05:05.69 Forearm.reconfigure LooseForearm @11:05:05.73 Forearm.seek :retract @11:05:07.34 Connecting PS to CS to transfer sample @11:05:07.35 CBV.dial AIV @11:05:07.36 AIV.dial :bypass @11:05:07.37 PSV.dial :mixloop @11:05:07.39 PTV.dial :PRVmixing @11:05:07.40 PRV.dial AIV @11:05:07.41 CTV.dial :air,avoiding: :intake @11:05:07.43 Loading lyfil puck from tube 2 into Collection Clamp @11:05:07.69 SC.reconfigure SCtight @11:05:07.74 SC.seek 2 @11:05:09.87 SC.reconfigure SCloose @11:05:10.11 Elbow.reconfigure TightElbow @11:05:10.16 Elbow.seek 2 @11:05:12.27 Forearm.reconfigure TightForearm @11:05:12.32 Forearm.seek 2 + 700 counts @11:05:13.38 SE.reconfigure SEpick @11:05:13.46 SE.seek beamBreak @11:05:15.51 SE.configure SEconfig @11:05:15.67 Elbow.seek 2 @11:05:15.87 Forearm.seek 2 + 300 counts @11:05:16.10 Forearm.reconfigure LooseForearm @11:05:16.14 Hand.close @11:05:16.76 SE.seek :down @11:05:17.00
Forearm.seek :retract @11:05:17.95 Elbow.seek CC @11:05:18.56 SE.coast @11:05:19.62
Elbow.reconfigure LooseElbow @11:05:19.73 Forearm.reconfigure TightForearm @11:05:19.78 Forearm.seek CC @11:05:21.16 CC.seek :closed @11:05:25.44 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.92 @11:05:25.45 Hand.open @11:05:26.20 Forearm.reconfigure LooseForearm @11:05:26.24 Forearm.seek :retract @11:05:27.26 SC.coast @11:05:27.29 CTV.dial CSV,avoiding: :intake @11:05:28.63 CSV.dial CTV @11:05:28.94 CS.seek 5ml @11:05:42.66 delay 1:20 @11:07:02.70 CS.seek 2.5ml @11:07:10.04 delay 40 seconds @11:07:50.08 CS.seek 0ml @11:07:57.70 delay 1:00 @11:08:57.73 PSV.dial :air @11:08:58.20 PS.seek 2.5ml @11:09:24.33 PSV.dial :mixloop @11:09:24.78 delay 3 seconds @11:09:27.80 PSV.dial :air @11:09:28.25 delay 2 seconds @11:09:30.27 PSV.dial :mixloop @11:09:30.74 delay 3 seconds @11:09:33.76 PSV.dial :waste1 @11:09:35.06 PS.seek :empty @11:10:01.17 CBV.dial :waste1 @11:10:01.67 delay 5 seconds @11:10:06.70 CBV.dial AIV @11:10:07.65 delay 10 seconds @11:10:17.76 CBV.dial :waste1 @11:10:18.25 delay 10 seconds @11:10:28.28 CBV.dial AIV @11:10:28.78 delay 10 seconds @11:10:38.81 CTV.dial CSV,avoiding: :intake @11:10:38.82 CBV.dial between AIV and waste1 @11:10:39.18 CSV.dial :air @11:10:39.66 CS.seek 10ml @11:11:06.17 CSV.dial CTV @11:11:06.63 CS.seek 0ml @11:11:33.23 CBV.dial :waste1 @11:11:33.56 delay 10 seconds @11:11:43.59 CTV.dial :air,avoiding: :intake @11:11:45.00 Storing lyfil puck in Collection Clamp to tube 1 @11:11:45.18 SC.reconfigure SCtight @11:11:45.23 SC.seek 1 @11:11:47.33 SC.reconfigure SCloose @11:11:47.55 Elbow.reconfigure TightElbow @11:11:47.60 Elbow.seek 1 @11:11:49.46 Forearm.reconfigure TightForearm @11:11:49.52 Forearm.seek 1 + 700 counts @11:11:50.58 SE.reconfigure SEpick @11:11:50.66 SE.seek beamBreak @11:11:52.75 SE.configure SEconfig @11:11:52.92 Elbow.reconfigure LimpElbow @11:11:52.98 Forearm.reconfigure LooseForearm @11:11:53.02 Forearm.seek 1 @11:11:53.29 Elbow.hold @11:11:53.36 Elbow.reconfigure TightElbow @11:11:53.47 SE.seek 9.07 Pucks below Top Plate @11:11:53.93 Forearm.force -20 @11:11:53.98 Forearm.seek 1 + 349 counts @11:11:54.29 Forearm.reconfigure TightForearm @11:11:54.35 Forearm.seek 1 + 700 counts @11:11:54.63 SE.seek 10.07 Pucks below Top Plate @11:11:55.24 Forearm.reconfigure LooseForearm @11:11:55.29 Forearm.seek :retract @11:11:56.19 Elbow.seek CC @11:11:57.93 Elbow.reconfigure LooseElbow @11:11:58.03 Forearm.seek CC @11:11:59.05 Hand.close @11:11:59.63 CC.seek :open @11:12:04.14 Forearm.seek :retract @11:12:05.19 Elbow.reconfigure TightElbow @11:12:05.24 Elbow.seek 1 @11:12:07.26 Elbow.reconfigure LimpElbow @11:12:07.31 Forearm.seek 1 @11:12:08.34 Elbow.hold @11:12:08.41 Elbow.reconfigure TightElbow @11:12:08.46 Hand.open @11:12:09.26 Forearm.force -20 @11:12:09.30 Forearm.seek 1 + 342 counts @11:12:10.16 Forearm.reconfigure TightForearm @11:12:10.22 Forearm.seek 1 + 700 counts @11:12:10.60 SE.seek :down @11:12:11.75
Forearm.reconfigure LooseForearm @11:12:11.80 Forearm.seek :retract @11:12:12.46 SE.coast @11:12:12.77
SC.coast @11:12:12.81 PSV.dial :air @11:12:13.90 PS.seek 0.499ml @11:12:19.77 delay 3 seconds @11:12:22.79 PSV.dial :mixloop @11:12:23.24 PRV.dial :air @11:12:23.72 delay 3 seconds @11:12:26.74 PRV.dial CBV @11:12:27.21 CBV.dial AIV @11:12:27.70 PRV.dial between CBV and air @11:12:28.04 PRV.dial :air @11:12:28.43 PS.reconfigure PSslow @11:12:28.48 PS.seek 0.599ml @11:12:31.05 PRV.dial :waste2 @11:12:31.79 PS.reconfigure PSconfig @11:12:31.84 PS.seek :empty @11:12:38.86 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:12:38.94 Hand.open @11:12:39.26 Elbow.seek FlushPuck::Garage @11:12:41.33 Elbow.reconfigure LooseElbow @11:12:41.44 Forearm.seek :garage @11:12:42.41 Hand.close @11:12:43.14 Forearm.seek :retract @11:12:44.14 Elbow.reconfigure TightElbow @11:12:44.19 Elbow.seek CC @11:12:47.08 Elbow.reconfigure LooseElbow @11:12:47.19 Forearm.reconfigure TightForearm @11:12:47.23 Forearm.seek CC @11:12:48.55 CC.seek :closed @11:12:52.83 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.90 @11:12:52.90 Hand.open @11:12:53.58 Forearm.reconfigure LooseForearm @11:12:53.62 Forearm.seek :retract @11:12:54.69 Clearing PML for modified lysate @11:12:54.70 CTV.dial :air,avoiding: :intake @11:12:54.71 PRV.dial :waste2 @11:12:54.72 PTV.dial :PRV,avoiding: :puck @11:12:55.49 delay 2 seconds @11:12:57.51 PTV.dial :PRVmixing,avoiding: :puck @11:12:58.27 delay 2 seconds @11:13:00.29 PSV.dial :PML @11:13:00.31 delay 2 seconds @11:13:02.33 PRV.dial :air @11:13:03.65 delay 2 seconds @11:13:05.73 PS.seek 1.499ml @11:13:21.74 delay 5 seconds @11:13:26.78 PS.seek 2.5ml @11:13:37.74 PSV.dial :air @11:13:38.20 PS.seek 0ml @11:14:04.40 mixing lysate with speDil @11:14:04.41 PSV.dial :PML @11:14:04.88 CBV.dial :PRV @11:14:04.89 PRV.dial :air @11:14:04.91 delay 2 seconds @11:14:06.93 PRV.dial :speDil @11:14:07.41 delay 2 seconds @11:14:09.49 PS.reconfigure PSslow1 @11:14:09.55 PS.seek 0.225ml @11:14:32.90 delay 5 seconds @11:14:37.93 PRV.dial CBV @11:14:38.71 delay 2 seconds @11:14:40.79 PS.seek 0.475ml @11:15:06.52 delay 5 seconds @11:15:11.55 PRV.dial :air @11:15:12.04 delay 2 seconds @11:15:14.07 PS.seek 0.965ml @11:16:03.96 delay 10 seconds @11:16:13.99 PRV.dial CBV @11:16:14.46 CTV.dial :air,avoiding: :intake @11:16:14.47 PSV.dial :air @11:16:14.93 PS.reconfigure PSconfig @11:16:14.99 PS.seek 2.5ml @11:16:31.37 delay 3 seconds @11:16:34.39 PSV.dial PTV @11:16:35.15 PTV.dial :PRV,avoiding: :puck @11:16:35.91 PS.seek 0ml @11:17:02.09 PSV.dial :air @11:17:02.85 PS.seek 2.5ml @11:17:28.99 delay 3 seconds @11:17:32.01 PSV.dial PTV @11:17:32.74 PTV.dial :PRV,avoiding: :puck @11:17:32.76 PS.seek 0ml @11:17:58.90 Pushing modified lysate into AIV Injection Loop @11:17:58.91 CBV.dial AIV @11:17:58.92 AIV.dial :bypass @11:17:58.93 PRV.dial CBV @11:17:58.95 PSV.dial :air @11:17:59.69 PS.seek 2.5ml @11:18:25.78 delay 3 seconds @11:18:28.80 PSV.dial :PML @11:18:29.26 PTV.dial :PRVmixing,avoiding: :puck @11:18:30.08 PS.reconfigure PSslow1 @11:18:30.13 PS.seek 1.74ml @11:19:47.46 delay 5 seconds @11:19:52.49 AIV.dial :sample @11:19:53.09 AWV.select :waste @11:19:53.26 PS.reconfigure PSslow2 @11:19:53.31 PS.seek 1.22ml @11:21:40.03 delay 15 seconds @11:21:55.06 AIV.dial :bypass @11:21:55.65 PS.reconfigure PSconfig @11:21:55.70 PS.seek 0ml @11:22:08.81 PSV.dial :air @11:22:09.27 PS.seek 2.5ml @11:22:35.34 PSV.dial :PML @11:22:35.81 PS.seek 0ml @11:23:02.00 AIV.dial :coilToMFB @11:23:02.01 AWV.select :air @11:23:02.18 Extracting Nucleic Acid from AIV @11:23:02.21 SPE.hold 2:46:40,55C @11:23:02.24
Beginning samplePassD @11:23:02.30 ASV.dial :SPEdelivery @11:23:02.42
AWV.select :air @11:23:02.43 AIV.dial :bypass @11:23:02.45 PSV.dial PSR @11:23:02.74 delay 20 seconds @11:23:03.57
Pulling 1.5ml of PSR.killMFB @11:23:03.58 PSR.select :killMFB @11:23:04.25 PS.seek 1.5ml @11:23:20.31 delay 5 seconds @11:23:22.77 primeLysateINT @11:23:22.78 AWV.select :air @11:23:22.79 AIV.dial :coilToMFB @11:23:22.81 ATV.dial AIV @11:23:24.46 AS.reconfigure ASslow1 @11:23:24.51 AS.seek 0.299ml @11:23:25.33
PSV.dial :waste1 @11:23:25.82 PS.seek 0ml @11:23:41.84 PSV.dial PSR @11:23:42.33 PSR.select :air @11:23:42.46 PS.seek 2.5ml @11:24:08.53 PRV.dial CBV @11:24:08.56
CBV.dial PRV @11:24:08.58 PSV.dial :mixloop @11:24:09.63 CTV.dial :air,avoiding: :intake @11:24:09.64 PTV.dial :PRVmixing @11:24:09.67 PS.seek 0ml @11:24:25.08 delay 10 seconds @11:24:35.11 ATV.dial :air2 @11:24:35.81
PSV.dial PSR @11:24:35.95 AS.seek 0.329ml @11:24:36.95
Pulling 1.5ml of PSR.flush @11:24:36.96 PSR.select :flush @11:24:37.08 PS.seek 1.5ml @11:24:42.27 ATV.dial :waste2 @11:24:42.81 AS.reconfigure ASnorm @11:24:42.86 AS.seek :empty @11:24:53.16
delay 5 seconds @11:24:58.19 PSV.dial :waste1 @11:24:58.71 PS.seek 0ml @11:24:59.88 Lysate primed! @11:24:59.89 AFV.select :holdingCoil @11:24:59.90 ATV.dial :air2 @11:25:00.51 AS.reconfigure ASslow1 @11:25:00.56 AS.seek 0.069ml @11:25:14.69
PSV.dial PSR @11:25:14.91 delay 3 seconds @11:25:15.28
PSR.select :air @11:25:15.41 PS.seek 2.5ml @11:25:17.93 ATV.dial :AIV @11:25:18.74 AS.seek 0.469ml @11:25:41.60 PRV.dial CBV @11:25:41.63
CBV.dial PRV @11:25:41.64 PSV.dial :mixloop @11:25:42.72 CTV.dial :air,avoiding: :intake @11:25:42.73 PTV.dial :PRVmixing @11:25:42.75 PS.seek 0ml @11:26:08.88 PSV.dial PSR @11:26:10.00 Pulling 1.5ml of PSR.flush @11:26:10.01 PSR.select :flush @11:26:10.14 PS.seek 1.5ml @11:26:26.16 delay 5 seconds @11:26:31.19 PSV.dial :waste1 @11:26:31.68 PS.seek 0ml @11:26:39.41 delay 5 seconds @11:26:44.50 Solid Phase Extraction Heater at 48.1C @11:26:44.51 ATV.dial ASV @11:26:44.89 ASV.dial :SPEdelivery @11:26:44.97 AS.seek 0.041ml @11:26:47.72
PSV.dial PSR @11:26:48.19 PSR.select :air @11:26:48.32 PS.seek 2.5ml @11:27:14.44 PRV.dial CBV @11:27:14.47
CBV.dial PRV @11:27:14.48 PSV.dial :mixloop @11:27:15.52 CTV.dial :air,avoiding: :intake @11:27:15.53 PTV.dial :PRVmixing @11:27:15.55 PS.seek 0ml @11:27:41.72 PSV.dial PSR @11:27:42.81 Pulling 1.5ml of PSR.flush @11:27:42.82 PSR.select :flush @11:27:42.95 PS.seek 1.5ml @11:27:58.94 delay 5 seconds @11:28:03.97 PSV.dial :waste1 @11:28:05.12 PS.seek 0ml @11:28:11.33 delay 10 seconds @11:28:21.19
PSV.dial PSR @11:28:21.35 rinseLysateLineINT @11:28:21.36 AFV.select :carrierSolution @11:28:21.47 delay 2 seconds @11:28:21.77
PSR.select :air @11:28:21.90 PS.seek 2.5ml @11:28:23.50 AS.reconfigure ASconfig @11:28:23.55 AS.seek 1ml @11:28:48.07 PRV.dial CBV @11:28:48.10
CBV.dial PRV @11:28:48.11 PSV.dial :mixloop @11:28:49.16 CTV.dial :air,avoiding: :intake @11:28:49.17 PTV.dial :PRVmixing @11:28:49.19 PS.reconfigure PSslow @11:28:49.24 PS.seek 1.2ml @11:29:16.01 PSV.dial :waste1 @11:29:17.33 PS.reconfigure PSconfig @11:29:17.38 PS.seek 0ml @11:29:18.96 delay 1 second @11:29:19.98 AFV.select :holdingCoil @11:29:20.08 delay 1 second @11:29:21.10 ATV.dial AIV @11:29:21.63 AIV.dial :coilToMFB @11:29:21.64 AWV.select :waste @11:29:21.76 AS.reconfigure ASslow0 @11:29:21.81 AS.seek 0ml @11:29:30.41
PSV.dial :air @11:29:31.56 PS.seek 2.5ml @11:29:57.69 PSV.dial :mixloop @11:29:58.16 PS.reconfigure PSslow @11:29:58.21 PS.seek 0ml @11:30:49.29 PSV.dial :air @11:30:49.76 PS.reconfigure PSconfig @11:30:49.81 PS.seek 2.5ml @11:31:02.71 ATV.dial :air2 @11:31:03.36 AS.reconfigure ASslow1 @11:31:03.41 AS.seek 0.05ml @11:31:13.77 ATV.dial :AIV @11:31:14.56 AS.seek 0.01ml @11:31:15.98
PSV.dial :mixloop @11:31:16.45 PS.reconfigure PSslow @11:31:16.50 PS.seek 0ml @11:31:22.91 ATV.dial :waste @11:31:24.46 AS.seek :empty @11:31:26.90 ethanol wash @11:31:26.91 ATV.dial :air2 @11:31:28.12 AS.reconfigure ASslow2 @11:31:28.17 AS.seek 0.009ml @11:31:38.54 delay 3 seconds @11:31:41.56 Pulling 0.4ml of Ethanol Wash @11:31:41.57 ATV.dial :ethanol @11:31:42.36 AS.reconfigure ASslow1 @11:31:42.41 AS.seek 0.409ml @11:32:07.54
Cleaning Collection Syringe @11:32:07.55 CSV.dial CSR @11:32:08.07 Pulling 4ml of CSR.flush @11:32:08.08 CSR.select :flush @11:32:08.21 CS.seek 4ml @11:32:19.45 delay 5 seconds @11:32:24.48 CSR.select :air @11:32:24.61 CS.seek 10ml @11:32:40.95 CSV.dial CTV @11:32:40.97
CTV.dial CSV @11:32:42.38 CBV.dial :waste1 @11:32:42.88 CS.seek 0ml @11:33:03.16 delay 3 seconds @11:33:06.19 ATV.dial :air1 @11:33:06.59 delay 3 seconds @11:33:09.42
CSV.dial CSR @11:33:09.67 AS.seek 0.434ml @11:33:09.94
Pulling 4ml of CSR.flush @11:33:09.95 CSR.select :flush @11:33:10.08 CS.seek 4ml @11:33:14.97 delay 3 seconds @11:33:17.99 ATV.dial :ethanol @11:33:18.58 AS.seek 0.419ml @11:33:21.92
delay 5 seconds @11:33:21.96 delay 3 seconds @11:33:24.98 ATV.dial ASV @11:33:26.28 AS.seek 0.092ml @11:33:26.94
CSR.select :air @11:33:27.07 CS.seek 10ml @11:33:43.39 CSV.dial CTV @11:33:43.42
CTV.dial CSV @11:33:43.92 CBV.dial :waste1 @11:33:43.94 CS.seek 0ml @11:34:10.58 Garaging FlushPuck in Collection Clamp @11:34:10.65 Elbow.reconfigure TightElbow @11:34:10.70 Elbow.seek CC @11:34:10.98 Elbow.reconfigure LooseElbow @11:34:11.08 Forearm.seek CC @11:34:12.09 Hand.close @11:34:12.68 CC.seek :open @11:34:17.05 Forearm.seek :retract @11:34:18.05 Elbow.reconfigure TightElbow @11:34:18.10 Elbow.seek :garage @11:34:21.33 Elbow.reconfigure LooseElbow @11:34:21.44 Forearm.reconfigure TightForearm @11:34:21.49 Forearm.seek :garage @11:34:22.64 Hand.open @11:34:23.37 Forearm.reconfigure LooseForearm @11:34:23.42 Forearm.seek :retract @11:34:24.45 Whole Cell Archive sampling 1500ml @11:34:24.47 Loading wcr puck from tube 2 into Collection Clamp @11:34:24.73 SC.reconfigure SCtight @11:34:24.78 SC.seek 2 @11:34:26.94 SC.reconfigure SCloose @11:34:27.17 Elbow.reconfigure TightElbow @11:34:27.22 Elbow.seek 2 @11:34:29.39 Forearm.reconfigure TightForearm @11:34:29.44 Forearm.seek 2 + 700 counts @11:34:30.49 SE.reconfigure SEpick @11:34:30.57 SE.seek beamBreak @11:34:32.29 delay 3 seconds @11:34:32.62 SE.configure SEconfig @11:34:32.79 Elbow.seek 2 @11:34:33.00 Forearm.seek 2 + 300 counts @11:34:33.27 Forearm.reconfigure LooseForearm @11:34:33.32 Hand.close @11:34:33.94 SE.seek :down @11:34:34.18 Forearm.seek :retract @11:34:35.13 Elbow.seek CC @11:34:35.31 ATV.dial :waste2 @11:34:35.88 SE.coast @11:34:36.15 AS.reconfigure ASconfig @11:34:36.20 AS.seek :empty @11:34:36.82 Elbow.reconfigure LooseElbow @11:34:37.49 Forearm.reconfigure TightForearm @11:34:37.55 Forearm.seek CC @11:34:38.89 CC.seek :closed @11:34:41.87 ATV.dial :air2 @11:34:42.28 AS.reconfigure ASslow1 @11:34:42.36 AS.seek 1ml @11:34:43.19 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 1.89 @11:34:43.20 Hand.open @11:34:43.87 Forearm.reconfigure LooseForearm @11:34:43.92 Forearm.seek :retract @11:34:44.92 SC.coast @11:34:44.96 collectSample 1500ml w/bubble point of 55psi @11:34:44.98 Priming 7ml @11:34:44.99 Intake.select :close @11:34:45.00 Exhaust.select :close @11:34:45.01 SS.hold @11:34:45.25 Calibrating pressure sensors @11:34:45.26 CTV.dial :air @11:34:46.61 SSV.dial :spit,avoiding: :exhaust @11:34:46.63 SS.reconfigure SSfast @11:34:46.68 SS.seek 5ml @11:34:49.46 SSV.dial CBV @11:34:49.49 CBV.dial SSV @11:34:50.96 delay 3.8 seconds @11:34:54.90 SSV.dial :spit @11:34:56.25 SS.seek 0ml @11:34:58.73 SSV.dial CBV @11:35:00.09 CTV.dial :intake @11:35:00.86 SSV.dial CBV @11:35:00.89 CBV.dial SSV @11:35:00.91 Allowing 40s for 7.0ml of remaining 7.0ml @11:35:00.92 Intake.select :open @11:35:03.10 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:35:03.12 SS.reconfigure SSinhale.with maxSpeed: 150 @11:35:03.17 SS.seek 7ml @11:35:09.04 Stroke completed -- waiting for pressure to equalize ... @11:35:22.93 Intake.select :close @11:35:23.09 SSV.dial :exhaust @11:35:23.89 Exhaust.select :open @11:35:24.01 SS.reconfigure SSexhale @11:35:24.06 SS.seek 0ml @11:35:27.73 Exhaust.select :close @11:35:27.84 Sampling 1500.0ml @11:35:27.86 Intake.select :close @11:35:27.87 Exhaust.select :close @11:35:27.88 SS.reconfigure SSconfig @11:35:27.93 SS.hold @11:35:28.15 CTV.dial :intake @11:35:28.17 SSV.dial CBV @11:35:28.21 CBV.dial SSV @11:35:29.01 Allowing 130s for 25.0ml of remaining 1500.0ml @11:35:29.02 Intake.select :open @11:35:31.21 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:35:31.23 SS.reconfigure SSinhale.with maxSpeed: 150 @11:35:31.28 SS.seek 25ml @11:35:34.99 Vacuum too high -- waiting up to 40s @11:35:37.61 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:35:37.63 SS.reconfigure SSinhale.with maxSpeed: 135 @11:35:37.68 SS.seek 25ml @11:35:40.61 Vacuum too high -- waiting up to 40s @11:35:43.23 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:35:43.25 SS.reconfigure SSinhale.with maxSpeed: 122 @11:35:43.30 SS.seek 25ml @11:35:45.85 Vacuum too high -- waiting up to 40s @11:35:48.47 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:35:48.49 SS.reconfigure SSinhale.with maxSpeed: 110 @11:35:48.53 SS.seek 25ml @11:35:50.83 Vacuum too high -- waiting up to 40s @11:35:53.30 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:35:53.32 SS.reconfigure SSinhale.with maxSpeed: 99 @11:35:53.37 SS.seek 25ml @11:35:56.27 Vacuum too high -- waiting up to 40s @11:35:58.69 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:35:58.71 SS.reconfigure SSinhale.with maxSpeed: 89 @11:35:58.77 SS.seek 25ml @11:36:01.64 Vacuum too high -- waiting up to 40s @11:36:03.86 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:36:03.88 SS.reconfigure SSinhale.with maxSpeed: 80 @11:36:03.93 SS.seek 25ml @11:36:06.45 Vacuum too high -- waiting up to 40s @11:36:08.56 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:36:08.58 SS.reconfigure SSinhale.with maxSpeed: 72 @11:36:08.63 SS.seek 25ml @11:36:11.55 Vacuum too high -- waiting up to 40s @11:36:13.78 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:36:13.80 SS.reconfigure SSinhale.with maxSpeed: 65 @11:36:13.85 SS.seek 25ml @11:36:16.96 Stroke completed -- waiting for pressure to equalize ... @11:36:22.65 Intake.select :close @11:36:22.81 SSV.dial :exhaust @11:36:23.62 Exhaust.select :open @11:36:23.74 SS.reconfigure SSexhale @11:36:23.79 SS.seek 0ml @11:36:32.78 Exhaust.select :close @11:36:32.90 SSV.dial CBV @11:36:32.92 CBV.dial SSV @11:36:33.75 Allowing 130s for 25.0ml of remaining 1475.0ml @11:36:33.77 Intake.select :open @11:36:35.94 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:36:35.96 SS.reconfigure SSinhale.with maxSpeed: 65 @11:36:36.01 SS.seek 25ml @11:36:41.42 Vacuum too high -- waiting up to 40s @11:36:43.28 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:36:43.30 SS.reconfigure SSinhale.with maxSpeed: 59 @11:36:43.35 SS.seek 25ml @11:36:48.17 Vacuum too high -- waiting up to 40s @11:36:50.04 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:36:50.06 SS.reconfigure SSinhale.with maxSpeed: 53 @11:36:50.11 SS.seek 25ml @11:36:56.61 Vacuum too high -- waiting up to 40s @11:36:58.46 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:36:58.48 SS.reconfigure SSinhale.with maxSpeed: 48 @11:36:58.53 SS.seek 25ml @11:37:05.74 Vacuum too high -- waiting up to 40s @11:37:07.57 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:37:07.59 SS.reconfigure SSinhale.with maxSpeed: 43 @11:37:07.64 SS.seek 25ml @11:37:20.55 Vacuum too high -- waiting up to 40s @11:37:22.96 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:37:22.98 SS.reconfigure SSinhale.with maxSpeed: 39 @11:37:23.03 SS.seek 25ml @11:37:23.68 Stroke completed -- waiting for pressure to equalize ... @11:37:26.29 Intake.select :close @11:37:26.45 SSV.dial :exhaust @11:37:27.25 Exhaust.select :open @11:37:27.37 SS.reconfigure SSexhale @11:37:27.42 SS.seek 0ml @11:37:36.32 Exhaust.select :close @11:37:36.43 SSV.dial CBV @11:37:36.46 CBV.dial SSV @11:37:37.28 Allowing 130s for 25.0ml of remaining 1450.0ml @11:37:37.29 Intake.select :open @11:37:39.47 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:37:39.49 SS.reconfigure SSinhale.with maxSpeed: 39 @11:37:39.54 SS.seek 25ml @11:38:03.95 delay 5 seconds @11:38:08.98 ATV.dial ASV @11:38:09.93 ASV.dial :SPEdelivery @11:38:09.95 AS.seek 0.02ml @11:38:21.96 Vacuum too high -- waiting up to 40s @11:38:23.82 Intake @31.0psia, vacuum from 20.0 to 24.0psi @11:38:23.84 SS.reconfigure SSinhale.with maxSpeed: 35 @11:38:23.89 SS.seek 25ml @11:38:27.18 Stroke completed -- waiting for pressure to equalize ... @11:38:31.02 Intake.select :close @11:38:31.18 SSV.dial :exhaust @11:38:31.97 Exhaust.select :open @11:38:32.09 SS.reconfigure SSexhale @11:38:32.14 SS.seek 0ml @11:38:41.09 Exhaust.select :close @11:38:41.21 SSV.dial CBV @11:38:41.23 CBV.dial SSV @11:38:42.04 Allowing 130s for 25.0ml of remaining 1425.0ml @11:38:42.05 Intake.select :open @11:38:44.23 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:38:44.25 SS.reconfigure SSinhale.with maxSpeed: 35 @11:38:44.30 SS.seek 25ml @11:39:34.39 Stroke completed -- waiting for pressure to equalize ... @11:39:39.48 Intake.select :close @11:39:39.64 SSV.dial :exhaust @11:39:40.43 Exhaust.select :open @11:39:40.55 SS.reconfigure SSexhale @11:39:40.60 SS.seek 0ml @11:39:49.56 Exhaust.select :close @11:39:50.26 SSV.dial CBV @11:39:50.28 CBV.dial SSV @11:39:51.11 Allowing 130s for 25.0ml of remaining 1400.0ml @11:39:51.12 Intake.select :open @11:39:53.31 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:39:53.33 SS.reconfigure SSinhale.with maxSpeed: 35 @11:39:53.37 SS.seek 25ml @11:40:43.65 Stroke completed -- waiting for pressure to equalize ... @11:40:49.44 Intake.select :close @11:40:49.60 SSV.dial :exhaust @11:40:50.40 Exhaust.select :open @11:40:50.52 SS.reconfigure SSexhale @11:40:50.57 SS.seek 0ml @11:40:59.56 Exhaust.select :close @11:40:59.68 SSV.dial CBV @11:40:59.70 CBV.dial SSV @11:41:00.54 Allowing 130s for 25.0ml of remaining 1375.0ml @11:41:00.55 Intake.select :open @11:41:02.74 Intake @31.1psia, vacuum from 20.0 to 24.0psi @11:41:02.76 SS.reconfigure SSinhale.with maxSpeed: 35 @11:41:02.81 SS.seek 25ml @11:41:27.31 delay 5 seconds @11:41:32.34 ATV.dial :air2 @11:41:33.37 AS.seek 0.52ml @11:41:52.92 Stroke completed -- waiting for pressure to equalize ... @11:41:58.66 Intake.select :close @11:41:58.82 SSV.dial :exhaust @11:41:59.62 Exhaust.select :open @11:41:59.74 SS.reconfigure SSexhale @11:41:59.79 SS.seek 0ml @11:42:08.76 Exhaust.select :close @11:42:08.88 SSV.dial CBV @11:42:08.90 CBV.dial SSV @11:42:09.72 Allowing 130s for 25.0ml of remaining 1350.0ml @11:42:09.73 Intake.select :open @11:42:11.92 Intake @31.1psia, vacuum from 20.0 to 24.0psi @11:42:11.94 SS.reconfigure SSinhale.with maxSpeed: 35 @11:42:11.99 SS.seek 25ml @11:43:02.33 Stroke completed -- waiting for pressure to equalize ... @11:43:08.71 Intake.select :close @11:43:08.87 SSV.dial :exhaust @11:43:10.23 Exhaust.select :open @11:43:10.35 SS.reconfigure SSexhale @11:43:10.40 SS.seek 0ml @11:43:14.28 delay 5 seconds @11:43:19.16 Exhaust.select :close @11:43:19.28 SSV.dial CBV @11:43:19.31 CBV.dial SSV @11:43:19.37 ATV.dial ASV @11:43:20.14 Allowing 130s for 25.0ml of remaining 1325.0ml @11:43:20.16 Intake.select :open @11:43:20.33 ASV.dial :wasteR @11:43:20.71 AS.seek 0.3ml @11:43:22.34 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:43:22.36 SS.reconfigure SSinhale.with maxSpeed: 35 @11:43:22.41 SS.seek 25ml @11:44:05.19 delay 20 seconds @11:44:12.78 Stroke completed -- waiting for pressure to equalize ... @11:44:19.81 Intake.select :close @11:44:19.97 SSV.dial :exhaust @11:44:20.76 Exhaust.select :open @11:44:20.88 SS.reconfigure SSexhale @11:44:20.93 SS.seek 0ml @11:44:25.22 AS.seek 0.07ml @11:44:29.67 Exhaust.select :close @11:44:29.79 SSV.dial CBV @11:44:29.81 CBV.dial SSV @11:44:30.64 Allowing 130s for 25.0ml of remaining 1300.0ml @11:44:30.65 Intake.select :open @11:44:32.84 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:44:32.86 SS.reconfigure SSinhale.with maxSpeed: 35 @11:44:32.91 SS.seek 25ml @11:45:11.75 delay 20 seconds @11:45:21.08 Vacuum too high -- waiting up to 40s @11:45:23.44 Intake @30.9psia, vacuum from 20.0 to 24.0psi @11:45:23.46 SS.reconfigure SSinhale.with maxSpeed: 32 @11:45:23.51 SS.seek 25ml @11:45:26.38 Stroke completed -- waiting for pressure to equalize ... @11:45:30.83 Intake.select :close @11:45:30.99 SSV.dial :exhaust @11:45:31.78 Exhaust.select :open @11:45:31.83 ATV.dial :waste2 @11:45:31.90 SS.reconfigure SSexhale @11:45:31.95 SS.seek 0ml @11:45:33.11 AS.reconfigure ASconfig @11:45:33.19 AS.seek :empty @11:45:37.53 Elution @11:45:37.54 ATV.dial :air1 @11:45:38.62 AS.reconfigure ASslow1 @11:45:38.67 AS.seek 0.049ml @11:45:41.11 Exhaust.select :close @11:45:41.23 SSV.dial CBV @11:45:41.25 CBV.dial SSV @11:45:42.08 Allowing 130s for 25.0ml of remaining 1275.0ml @11:45:42.10 Intake.select :open @11:45:44.28 Intake @31.1psia, vacuum from 20.0 to 24.0psi @11:45:44.30 SS.reconfigure SSinhale.with maxSpeed: 32 @11:45:44.37 SS.seek 25ml @11:45:49.05 delay 3 seconds @11:45:52.08 ATV.dial :ElutionWater @11:45:52.74 AS.seek 0.109ml @11:46:05.11 delay 3 seconds @11:46:08.14 ATV.dial ASV,via: :air2 @11:46:10.12 ASV.dial :SPEelution @11:46:10.69 AFV.select :carrierSolution @11:46:10.85 AS.reconfigure ASnorm @11:46:10.91 AS.seek 0.759ml @11:46:39.37 Stroke completed -- waiting for pressure to equalize ... @11:46:43.99 delay 3 seconds @11:46:45.78 Intake.select :close @11:46:45.94 SSV.dial :exhaust @11:46:46.73 Exhaust.select :open @11:46:46.85 SS.reconfigure SSexhale @11:46:46.90 SS.seek 0ml @11:46:47.01 AFV.select :holdingCoil @11:46:47.16 AS.reconfigure ASslow1 @11:46:47.21 AS.seek 0.474ml @11:46:56.03 Exhaust.select :close @11:46:56.15 SSV.dial CBV @11:46:56.17 CBV.dial SSV @11:46:56.97 Allowing 130s for 25.0ml of remaining 1250.0ml @11:46:56.98 Intake.select :open @11:46:59.17 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:46:59.19 SS.reconfigure SSinhale.with maxSpeed: 32 @11:46:59.24 SS.seek 25ml @11:47:44.80 delay 5 seconds @11:47:49.90 AS.reconfigure ASslow2 @11:47:49.95 AS.seek 0.439ml @11:47:54.29 Stroke completed -- waiting for pressure to equalize ... @11:48:01.29 Intake.select :close @11:48:01.45 SSV.dial :exhaust @11:48:02.23 Exhaust.select :open @11:48:02.35 SS.reconfigure SSexhale @11:48:02.40 SS.seek 0ml @11:48:11.52 Exhaust.select :close @11:48:11.64 SSV.dial CBV @11:48:11.66 CBV.dial SSV @11:48:13.06 Allowing 130s for 25.0ml of remaining 1225.0ml @11:48:13.07 Intake.select :open @11:48:15.25 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:48:15.27 SS.reconfigure SSinhale.with maxSpeed: 32 @11:48:15.32 SS.seek 25ml @11:48:25.35 delay 1:00 @11:49:10.31 Stroke completed -- waiting for pressure to equalize ... @11:49:18.04 Intake.select :close @11:49:18.20 SSV.dial :exhaust @11:49:18.99 Exhaust.select :open @11:49:19.11 SS.reconfigure SSexhale @11:49:19.16 SS.seek 0ml @11:49:25.38 pushing eluate to holding coil @11:49:25.45 AS.seek 0.279ml @11:49:28.09 Exhaust.select :close @11:49:28.21 SSV.dial CBV @11:49:28.23 CBV.dial SSV @11:49:29.05 Allowing 130s for 25.0ml of remaining 1200.0ml @11:49:29.06 Intake.select :open @11:49:31.25 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:49:31.27 SS.reconfigure SSinhale.with maxSpeed: 32 @11:49:31.32 SS.seek 25ml @11:50:10.08 Vacuum too high -- waiting up to 40s @11:50:12.44 Intake @31.0psia, vacuum from 20.0 to 24.0psi @11:50:12.46 SS.reconfigure SSinhale.with maxSpeed: 29 @11:50:12.51 SS.seek 25ml @11:50:30.51 Stroke completed -- waiting for pressure to equalize ... @11:50:37.50 Intake.select :close @11:50:37.66 SSV.dial :exhaust @11:50:38.45 Exhaust.select :open @11:50:38.57 SS.reconfigure SSexhale @11:50:38.62 SS.seek 0ml @11:50:47.63 Exhaust.select :close @11:50:47.75 SSV.dial CBV @11:50:47.77 CBV.dial SSV @11:50:48.60 Allowing 130s for 25.0ml of remaining 1175.0ml @11:50:48.61 Intake.select :open @11:50:51.38 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:50:51.40 SS.reconfigure SSinhale.with maxSpeed: 29 @11:50:51.45 SS.seek 25ml @11:51:52.02 Stroke completed -- waiting for pressure to equalize ... @11:52:00.34 Intake.select :close @11:52:00.50 SSV.dial :exhaust @11:52:01.31 Exhaust.select :open @11:52:01.43 SS.reconfigure SSexhale @11:52:01.48 SS.seek 0ml @11:52:06.57 delay 30 seconds @11:52:10.55 Exhaust.select :close @11:52:10.66 SSV.dial CBV @11:52:10.69 CBV.dial SSV @11:52:11.50 Allowing 130s for 25.0ml of remaining 1150.0ml @11:52:11.51 Intake.select :open @11:52:13.70 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:52:13.72 SS.reconfigure SSinhale.with maxSpeed: 29 @11:52:13.76 SS.seek 25ml @11:52:36.60 ATV.dial :waste,via: :air3 @11:52:38.23 AS.reconfigure ASconfig @11:52:38.28 AS.seek :empty @11:52:54.65 Parking Bleach in LysateSource line and SPEcolumn @11:52:54.66 ATV.dial :air3 @11:52:55.47 AS.reconfigure ASslow1 @11:52:55.52 AS.seek 0.029ml @11:53:01.82 ATV.dial :ElutionWater @11:53:02.41 AS.seek 0.059ml @11:53:04.55 Vacuum too high -- waiting up to 40s @11:53:07.17 Intake @30.9psia, vacuum from 20.0 to 24.0psi @11:53:07.19 SS.reconfigure SSinhale.with maxSpeed: 26 @11:53:07.24 SS.seek 25ml @11:53:08.68 ATV.dial :air3 @11:53:09.28 AS.seek 0.069ml @11:53:11.52 delay 2 seconds @11:53:13.54 ATV.dial :bleach @11:53:14.00 AS.seek 0.769ml @11:53:18.07 Stroke completed -- waiting for pressure to equalize ... @11:53:25.05 Intake.select :close @11:53:25.21 SSV.dial :exhaust @11:53:26.00 Exhaust.select :open @11:53:26.12 SS.reconfigure SSexhale @11:53:26.17 SS.seek 0ml @11:53:35.16 Exhaust.select :close @11:53:35.28 SSV.dial CBV @11:53:35.30 CBV.dial SSV @11:53:36.13 Allowing 130s for 25.0ml of remaining 1125.0ml @11:53:36.14 Intake.select :open @11:53:38.33 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:53:38.35 SS.reconfigure SSinhale.with maxSpeed: 26 @11:53:38.39 SS.seek 25ml @11:54:45.75 Stroke completed -- waiting for pressure to equalize ... @11:54:54.06 Intake.select :close @11:54:54.22 SSV.dial :exhaust @11:54:55.02 Exhaust.select :open @11:54:55.14 SS.reconfigure SSexhale @11:54:55.19 SS.seek 0ml @11:55:04.30 Exhaust.select :close @11:55:04.42 SSV.dial CBV @11:55:04.44 CBV.dial SSV @11:55:05.27 Allowing 130s for 25.0ml of remaining 1100.0ml @11:55:05.28 Intake.select :open @11:55:07.47 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:55:07.49 SS.reconfigure SSinhale.with maxSpeed: 26 @11:55:07.54 SS.seek 25ml @11:55:35.02 delay 3 seconds @11:55:38.05 ATV.dial :air3 @11:55:38.59 delay 3 seconds @11:55:41.68 AS.seek 0.794ml @11:55:46.98 delay 3 seconds @11:55:50.01 ATV.dial :bleach @11:55:50.61 AS.seek 0.779ml @11:55:53.90 delay 3 seconds @11:55:56.93 ATV.dial :AIV @11:55:58.12 AS.reconfigure ASconfig @11:55:58.17 AS.seek 0.015ml @11:56:05.68 Vacuum too high -- waiting up to 40s @11:56:08.29 Intake @30.9psia, vacuum from 20.0 to 24.0psi @11:56:08.31 SS.reconfigure SSinhale.with maxSpeed: 23 @11:56:08.35 SS.seek 25ml @11:56:18.77 Stroke completed -- waiting for pressure to equalize ... @11:56:26.41 Intake.select :close @11:56:26.57 SSV.dial :exhaust @11:56:27.38 Exhaust.select :open @11:56:27.50 SS.reconfigure SSexhale @11:56:27.55 SS.seek 0ml @11:56:36.69 Exhaust.select :close @11:56:36.81 SSV.dial CBV @11:56:36.83 CBV.dial SSV @11:56:37.66 Allowing 130s for 25.0ml of remaining 1075.0ml @11:56:37.68 Intake.select :open @11:56:39.86 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:56:39.88 SS.reconfigure SSinhale.with maxSpeed: 23 @11:56:39.93 SS.seek 25ml @11:56:42.35 ATV.dial :waste @11:56:43.76 AS.seek :empty @11:56:44.98 ATV.dial :air3 @11:56:45.64 AS.reconfigure ASslow1 @11:56:45.69 AS.seek 0.009ml @11:56:48.02 delay 2 seconds @11:56:50.04 ATV.dial :bleach @11:56:50.49 AS.seek 0.579ml @11:57:56.10 Stroke completed -- waiting for pressure to equalize ... @11:58:05.63 Intake.select :close @11:58:05.79 SSV.dial :exhaust @11:58:06.58 Exhaust.select :open @11:58:06.70 SS.reconfigure SSexhale @11:58:06.75 SS.seek 0ml @11:58:15.55 Exhaust.select :close @11:58:15.67 SSV.dial CBV @11:58:15.69 CBV.dial SSV @11:58:16.52 Allowing 130s for 25.0ml of remaining 1050.0ml @11:58:16.53 Intake.select :open @11:58:18.72 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:58:18.74 SS.reconfigure SSinhale.with maxSpeed: 23 @11:58:18.79 SS.seek 25ml @11:58:45.37 delay 3 seconds @11:58:48.40 ATV.dial :air3 @11:58:48.93 delay 3 seconds @11:58:52.02 AS.seek 0.604ml @11:58:57.32 delay 3 seconds @11:59:00.34 ATV.dial :bleach @11:59:00.94 AS.seek 0.589ml @11:59:04.25 delay 3 seconds @11:59:07.28 ATV.dial :ASV @11:59:08.26 ASV.dial :wasteR @11:59:09.44 AS.seek 0ml @11:59:25.34 Vacuum too high -- waiting up to 40s @11:59:28.76 Intake @31.1psia, vacuum from 20.0 to 24.0psi @11:59:28.78 SS.reconfigure SSinhale.with maxSpeed: 21 @11:59:28.82 SS.seek 25ml @11:59:40.01 Stroke completed -- waiting for pressure to equalize ... @11:59:48.31 Intake.select :close @11:59:48.47 SSV.dial :exhaust @11:59:49.27 Exhaust.select :open @11:59:49.39 SS.reconfigure SSexhale @11:59:49.44 SS.seek 0ml @11:59:58.44 Exhaust.select :close @11:59:58.56 SSV.dial CBV @11:59:58.58 CBV.dial SSV @11:59:59.40 Allowing 130s for 25.0ml of remaining 1025.0ml @11:59:59.41 Intake.select :open @12:00:01.60 Intake @31.4psia, vacuum from 20.0 to 24.0psi @12:00:01.62 SS.reconfigure SSinhale.with maxSpeed: 21 @12:00:01.67 SS.seek 25ml @12:01:08.18 delay 1:00 @12:01:22.40 Vacuum too high -- waiting up to 40s @12:01:25.98 Intake @31.1psia, vacuum from 20.0 to 24.0psi @12:01:26.00 SS.reconfigure SSinhale.with maxSpeed: 19 @12:01:26.05 SS.seek 25ml @12:01:29.82 Stroke completed -- waiting for pressure to equalize ... @12:01:37.51 Intake.select :close @12:01:37.67 SSV.dial :exhaust @12:01:38.47 Exhaust.select :open @12:01:38.59 SS.reconfigure SSexhale @12:01:38.64 SS.seek 0ml @12:01:47.66 Exhaust.select :close @12:01:47.78 SSV.dial CBV @12:01:47.80 CBV.dial SSV @12:01:48.61 Allowing 130s for 25.0ml of remaining 1000.0ml @12:01:48.62 Intake.select :open @12:01:50.81 Intake @31.3psia, vacuum from 20.0 to 24.0psi @12:01:50.83 SS.reconfigure SSinhale.with maxSpeed: 19 @12:01:50.87 SS.seek 25ml @12:02:08.21 AFV.select :carrierSolution @12:02:08.31 delay 2 seconds @12:02:10.34 AS.reconfigure ASconfig @12:02:10.39 AS.seek 1ml @12:03:08.17 delay 1 second @12:03:09.19 AFV.select :holdingCoil @12:03:09.29 delay 1 second @12:03:10.32 ATV.dial ASV @12:03:10.33 ASV.dial :wasteR @12:03:10.35 AS.reconfigure ASslow1 @12:03:10.39 AS.seek 0ml @12:03:23.00 Stroke completed -- waiting for pressure to equalize ... @12:03:35.66 Intake.select :close @12:03:35.82 SSV.dial :exhaust @12:03:36.62 Exhaust.select :open @12:03:36.74 SS.reconfigure SSexhale @12:03:36.79 SS.seek 0ml @12:03:45.96 Exhaust.select :close @12:03:46.08 SSV.dial CBV @12:03:46.10 CBV.dial SSV @12:03:46.94 Allowing 130s for 25.0ml of remaining 975.0ml @12:03:46.96 Intake.select :open @12:03:49.14 Intake @31.4psia, vacuum from 20.0 to 24.0psi @12:03:49.16 SS.reconfigure SSinhale.with maxSpeed: 19 @12:03:49.21 SS.seek 25ml @12:04:50.46 Vacuum too high -- waiting up to 40s @12:04:54.23 Intake @31.2psia, vacuum from 20.0 to 24.0psi @12:04:54.25 SS.reconfigure SSinhale.with maxSpeed: 17 @12:04:54.30 SS.seek 25ml @12:05:29.44 Stroke completed -- waiting for pressure to equalize ... @12:05:42.74 Intake.select :close @12:05:42.90 SSV.dial :exhaust @12:05:43.69 Exhaust.select :open @12:05:43.81 SS.reconfigure SSexhale @12:05:43.86 SS.seek 0ml @12:05:52.93 Exhaust.select :close @12:05:53.05 SSV.dial CBV @12:05:53.07 CBV.dial SSV @12:05:53.90 Allowing 130s for 25.0ml of remaining 950.0ml @12:05:53.91 Intake.select :open @12:05:56.09 Intake @31.3psia, vacuum from 20.0 to 24.0psi @12:05:56.11 SS.reconfigure SSinhale.with maxSpeed: 17 @12:05:56.16 SS.seek 25ml @12:06:31.75 delay 20 seconds @12:06:51.78 ATV.dial :air3 @12:06:53.21 AS.seek 0.59ml @12:07:21.37 Vacuum too high -- waiting up to 40s @12:07:25.86 Intake @31.1psia, vacuum from 20.0 to 24.0psi @12:07:25.88 SS.reconfigure SSinhale.with maxSpeed: 15 @12:07:25.92 SS.seek 25ml @12:07:46.49 Stroke completed -- waiting for pressure to equalize ... @12:07:59.82 Intake.select :close @12:07:59.98 SSV.dial :exhaust @12:08:00.79 Exhaust.select :open @12:08:00.91 SS.reconfigure SSexhale @12:08:00.96 SS.seek 0ml @12:08:10.12 Exhaust.select :close @12:08:10.24 SSV.dial CBV @12:08:10.26 CBV.dial SSV @12:08:11.09 Allowing 130s for 25.0ml of remaining 925.0ml @12:08:11.10 Intake.select :open @12:08:13.29 Intake @31.4psia, vacuum from 20.0 to 24.0psi @12:08:13.31 SS.reconfigure SSinhale.with maxSpeed: 15 @12:08:13.35 SS.seek 25ml @12:08:52.13 delay 3 seconds @12:08:55.16 ATV.dial :ASV @12:08:56.50 ASV.dial :wasteR @12:08:56.58 AS.seek 0.09ml @12:10:10.23 Stroke completed -- waiting for pressure to equalize ... @12:10:21.11 SS.reconfigure SSconfig @12:10:21.16 SS.hold @12:10:21.30 Intake.select :close @12:10:21.46 ShallowSampler::Clogged in WCR -- progress too slow after 600.0ml @12:10:21.48 SSV.dial :exhaust @12:10:22.27 Exhaust.select :open @12:10:22.39 SS.reconfigure SSexhale @12:10:22.44 SS.seek 0ml @12:10:31.70 Exhaust.select :close @12:10:31.81 Sampled 600.0ml @12:10:31.83 Drying puck before fixing for 4:00 @12:10:31.84 CBV.dial :waste2b @12:10:32.62 delay 1:00 @12:10:37.42 delay 30 seconds @12:11:07.45 ATV.dial :extSrc @12:11:08.21 delay 3 seconds @12:11:11.24 ATV.dial :ElutionWater @12:11:12.31 AS.seek 0.12ml @12:11:18.64 ATV.dial :air3 @12:11:19.25 AS.seek 0.13ml @12:11:21.53 delay 2 seconds @12:11:23.55 ATV.dial :bleach @12:11:24.01 AS.seek 0.23ml @12:11:32.65 CSV.dial :air @12:11:33.12 CS.reconfigure CSfast @12:11:33.17 CS.seek 10ml @12:11:44.36 delay 3 seconds @12:11:47.39 ATV.dial :air3 @12:11:47.93 delay 3 seconds @12:11:51.02 AS.seek 0.255ml @12:11:56.22 Pumping wcr puck from tube 2 up to 55psi... @12:11:56.24 CBV.dial SSV @12:11:56.31 delay 3 seconds @12:11:57.62 CBV.dial between CSV and waste2b @12:11:58.64 CSV.dial between air and CTV @12:11:58.99 CS.seek 7.172ml @12:11:59.33 ATV.dial :bleach @12:11:59.93 AS.seek 0.24ml @12:12:03.24 delay 3 seconds @12:12:06.27 ATV.dial :ASV @12:12:06.34 CTV.dial CSV,avoiding: :air @12:12:06.37 CSV.dial CTV @12:12:07.23 CS.reconfigure CSpressurize @12:12:07.30 CS.seek 0ml @12:12:07.33 ASV.dial :SPEdelivery @12:12:07.75 AFV.select :carrierSolution @12:12:07.86 delay 2 seconds @12:12:09.89 AS.reconfigure ASconfig @12:12:09.94 AS.seek 0.5ml @12:12:25.18 delay 1 second @12:12:25.39 CBV.dial :waste2b @12:12:25.75 CS.reconfigure CSconfig @12:12:25.81 CS.seek :empty @12:12:26.20 AFV.select :holdingCoil @12:12:26.38 AS.reconfigure ASslow1 @12:12:26.43 AS.seek 0.17ml @12:12:27.50 delay 1:00 @12:13:27.53 CTV.dial :air,avoiding: :intake @12:13:28.87 Fix #1 of 2 (with rnal) for 2:00 @12:13:28.89 CSV.dial CSR @12:13:29.40 Pulling 2ml of CSR.rnal @12:13:29.42 CSR.select :rnal @12:13:29.54 CS.seek 2ml @12:13:33.13 SPE.hold 2:46:40,95C @12:13:33.19 delay 3:00 @12:13:35.75 delay 5 seconds @12:13:40.78 CSR.select :air @12:13:40.90 CS.reconfigure CSfast @12:13:40.95 CS.seek 10ml @12:14:00.09 Pumping wcr puck from tube 2 up to 55psi... @12:14:00.66 CBV.dial SSV @12:14:02.05 CBV.dial between CSV and waste2b @12:14:03.10 CSV.dial between air and CTV @12:14:03.76 CS.seek 7.12ml @12:14:11.32 CTV.dial CSV,avoiding: :air @12:14:11.36 CSV.dial CTV @12:14:12.81 CS.reconfigure CSpressurize @12:14:12.86 CS.seek 0ml @12:14:30.83 delay 2:00 @12:16:30.87 CBV.dial :waste2b @12:16:31.22 CS.reconfigure CSconfig @12:16:31.28 CS.seek :empty @12:16:32.97 delay 1:00 @12:16:33.23 AFV.select :carrierSolution @12:16:33.33 delay 2 seconds @12:16:35.36 AS.reconfigure ASconfig @12:16:35.41 AS.seek 1ml @12:17:23.46 delay 1 second @12:17:24.48 AFV.select :holdingCoil @12:17:24.58 delay 1 second @12:17:25.61 ASV.dial :wasteD @12:17:26.67 AS.seek 0ml @12:17:33.00 CTV.dial :air,avoiding: :intake @12:17:34.38 Fix #2 of 2 (with rnal) for 2:00 @12:17:34.40 CSV.dial CSR @12:17:34.93 Pulling 2ml of CSR.rnal @12:17:34.94 CSR.select :rnal @12:17:35.06 CS.seek 2ml @12:17:41.28 delay 5 seconds @12:17:46.31 CSR.select :air @12:17:46.44 CS.reconfigure CSfast @12:17:46.49 CS.seek 10ml @12:18:05.32 Pumping wcr puck from tube 2 up to 55psi... @12:18:05.34 CBV.dial SSV @12:18:06.70 CBV.dial between CSV and waste2b @12:18:07.70 CSV.dial between air and CTV @12:18:08.34 CS.seek 7.044ml @12:18:15.98 CTV.dial CSV,avoiding: :air @12:18:16.05 CSV.dial CTV @12:18:17.40 CS.reconfigure CSpressurize @12:18:17.45 CS.seek 0ml @12:18:24.45 Priming Eluate @12:18:24.46 AFV.select :carrierSolution @12:18:24.57 delay 2 seconds @12:18:26.61 AS.seek 0.5ml @12:18:35.84 delay 2:00 @12:18:55.66 delay 1 second @12:18:56.68 AFV.select :holdingCoil @12:18:56.78 ATV.dial ASV @12:18:56.79 ASV.dial :SPErecovery @12:18:57.37 Swish #1 of 2 @12:18:57.45 AS.reconfigure ASslow0 @12:18:57.50 AS.seek 0.6ml @12:19:07.94 AS.seek 0.5ml @12:19:18.31 Swish #2 of 2 @12:19:18.40 AS.seek 0.6ml @12:19:28.88 AS.seek 0.5ml @12:19:39.27 delay 3 seconds @12:19:42.35 AS.reconfigure ASslow1 @12:19:42.40 AS.seek 0.684ml @12:20:19.71 delay 3 seconds @12:20:22.73 Eluate primed @12:20:22.74 ATV.dial :waste2 @12:20:23.32 AS.reconfigure ASnorm @12:20:23.37 AS.seek 0ml @12:20:35.88 CBV.dial :waste2b @12:20:36.77 CS.reconfigure CSconfig @12:20:36.85 CS.seek :empty @12:20:37.73 delay 1:00 @12:20:58.21 SPE.coast @12:20:58.29 ASV.dial :SPErecovery @12:20:58.31 Setting up PCR for OM -- writing /var/log/esp/OM20may1612h625ml.pcr @12:20:58.33 Building PCR Slug from ASV with MM3 mix, PP5 primer @12:20:58.35 Priming with carrier @12:20:58.36 ATV.dial ARV @12:20:59.31 ARV.dial :carrier @12:20:59.67 AS.seek 0.5ml @12:21:25.26 AFV.select :carrierSolution @12:21:25.37 delay 2 seconds @12:21:27.40 AS.reconfigure ASconfig @12:21:27.45 AS.seek 1ml @12:21:37.76 CTV.dial :air,avoiding: :intake @12:21:39.17 Storing wcr puck in Collection Clamp to tube 1 @12:21:39.36 SC.reconfigure SCtight @12:21:39.42 SC.seek 1 @12:21:41.75 SC.reconfigure SCloose @12:21:41.99 Elbow.reconfigure TightElbow @12:21:42.04 Elbow.seek 1 @12:21:43.91 Forearm.reconfigure TightForearm @12:21:43.96 Forearm.seek 1 + 700 counts @12:21:45.02 SE.reconfigure SEpick @12:21:45.10 SE.seek beamBreak @12:21:47.23 SE.configure SEconfig @12:21:47.40 Elbow.reconfigure LimpElbow @12:21:47.46 Forearm.reconfigure LooseForearm @12:21:47.50 Forearm.seek 1 @12:21:47.84 Elbow.hold @12:21:47.92 Elbow.reconfigure TightElbow @12:21:48.02 SE.seek 10.09 Pucks below Top Plate @12:21:48.46 Forearm.force -20 @12:21:48.50 Forearm.seek 1 + 312 counts @12:21:48.83 Forearm.reconfigure TightForearm @12:21:48.88 Forearm.seek 1 + 700 counts @12:21:49.11 SE.seek 11.09 Pucks below Top Plate @12:21:49.74 Forearm.reconfigure LooseForearm @12:21:49.79 Forearm.seek :retract @12:21:50.74 Elbow.seek CC @12:21:52.38 Elbow.reconfigure LooseElbow @12:21:52.50 Forearm.seek CC @12:21:53.54 Hand.close @12:21:54.18 CC.seek :open @12:21:56.51 delay 1 second @12:21:57.53 AFV.select :holdingCoil @12:21:57.63 delay 1 second @12:21:58.68 Forearm.seek :retract @12:21:58.73 ATV.dial :waste @12:21:59.73 Elbow.reconfigure TightElbow @12:22:00.38 Elbow.seek 1 @12:22:00.46 AS.reconfigure ASnorm @12:22:00.51 AS.seek :empty @12:22:02.38 Elbow.reconfigure LimpElbow @12:22:02.44 Forearm.seek 1 @12:22:03.45 Elbow.hold @12:22:03.53 Elbow.reconfigure TightElbow @12:22:03.58 Hand.open @12:22:04.40 Forearm.force -20 @12:22:04.44 Forearm.seek 1 + 349 counts @12:22:04.79 Forearm.reconfigure TightForearm @12:22:04.84 Forearm.seek 1 + 700 counts @12:22:05.14 SE.seek :down @12:22:06.30 Forearm.reconfigure LooseForearm @12:22:06.36 Forearm.seek :retract @12:22:06.82 SE.coast @12:22:07.31 SC.coast @12:22:07.36 Starting finalPurge @12:22:07.37 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:22:07.45 Hand.open @12:22:07.90 Elbow.seek FlushPuck::Garage @12:22:09.95 Elbow.reconfigure LooseElbow @12:22:10.06 Forearm.seek :garage @12:22:11.07 Hand.close @12:22:11.77 Forearm.seek :retract @12:22:12.77 Elbow.reconfigure TightElbow @12:22:12.82 Elbow.seek CC @12:22:15.74 Elbow.reconfigure LooseElbow @12:22:15.84 Forearm.reconfigure TightForearm @12:22:15.89 Forearm.seek CC @12:22:17.21 CC.seek :closed @12:22:21.48 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.88 @12:22:21.56 Hand.open @12:22:22.20 Forearm.reconfigure LooseForearm @12:22:22.25 Forearm.seek :retract @12:22:23.26 Flushing the Collection Valve::Manifold @12:22:23.40 CSV.dial CSR @12:22:23.91 Pulling 5ml of CSR.flush @12:22:23.92 CSR.select :flush @12:22:24.05 CS.seek 5ml @12:22:37.88 delay 5 seconds @12:22:42.91 CSR.select :air @12:22:43.68 CS.reconfigure CSfast @12:22:43.73 CS.seek 7.5ml @12:22:50.29 CSV.dial :waste @12:22:51.26 AFV.select :carrierSolution @12:22:51.37 delay 2 seconds @12:22:51.59
CS.reconfigure CSconfig @12:22:51.64 CS.seek 0ml @12:22:53.40 AS.reconfigure ASconfig @12:22:53.46 AS.seek 0.5ml @12:23:11.79
Flushing lower manifold's waste1 port @12:23:11.93 CSV.dial :flush @12:23:12.41 CS.reconfigure CSfast @12:23:12.45 CS.seek 5ml @12:23:22.56 delay 1 second @12:23:23.58 AFV.select :holdingCoil @12:23:23.68 Building Slug @12:23:23.69 ATV.dial :ARV @12:23:24.45
delay 5 seconds @12:23:25.25 delay 2 seconds @12:23:27.28 ARV.dial :air1 @12:23:27.68 delay 2 seconds @12:23:29.47
CSV.dial :air @12:23:29.77 AS.reconfigure ASslow2 @12:23:29.82 AS.seek 0.506ml @12:23:30.00
CS.seek 7ml @12:23:35.51 CSV.dial CTV @12:23:35.54
CTV.dial CSV @12:23:36.18 delay 2 seconds @12:23:36.98
CBV.dial :waste1 @12:23:37.79 CS.seek 0ml @12:23:38.20 ARV.dial :MM3 @12:23:39.21 delay 2 seconds @12:23:41.24 Adding MM3 @12:23:41.32 AS.seek 0.524ml @12:23:54.07
Flushing lower manifold's waste2b port @12:23:54.21 CSV.dial :flush @12:23:54.97 CS.seek 5ml @12:23:59.61 delay 25 seconds @12:24:06.82
delay 5 seconds @12:24:11.85 CSV.dial :air @12:24:12.39 CS.seek 7ml @12:24:17.79 CSV.dial CTV @12:24:17.82
CTV.dial CSV @12:24:18.29 CBV.dial :waste2b @12:24:19.08 CS.seek 0ml @12:24:24.64 ARV.dial :PP5 @12:24:25.03 Adding PP5 @12:24:25.05 delay 2 seconds @12:24:27.13 AS.seek 0.53ml @12:24:33.39 delay 10 seconds @12:24:35.29
Flushing upper manifold @12:24:35.43 CSV.dial :flush @12:24:36.19 CS.seek 5ml @12:24:43.42 ARV.dial :air3 @12:24:43.79 adding ARV air @12:24:43.81 delay 2 seconds @12:24:45.89 AS.seek 0.536ml @12:24:48.11
delay 5 seconds @12:24:52.06 delay 5 seconds @12:24:53.13
CSV.dial :air @12:24:53.66 CS.seek 7ml @12:24:57.08 ARV.dial :carrier @12:24:58.29 delay 7 seconds @12:24:59.11 CSV.dial CBV @12:24:59.15
CBV.dial CSV @12:25:00.23 CTV.dial :air @12:25:01.55 CS.seek 0ml @12:25:05.38 AS.seek 0.594ml @12:25:17.70
delay 7 seconds @12:25:24.74 CS.seek 3ml @12:25:32.39 CSV.dial :waste @12:25:32.87 CS.seek 0ml @12:25:41.18 CSV.dial :flush @12:25:41.65 CS.seek 3ml @12:25:49.20 delay 5 seconds @12:25:54.23 CSV.dial :air @12:25:54.76 CS.seek 5ml @12:26:00.23 CSV.dial :air @12:26:00.25 CS.seek 0ml @12:26:03.84 delay 5 seconds @12:26:08.87 ATV.dial :ASV @12:26:09.25 delay 2 seconds @12:26:11.35 AS.seek 0.6ml @12:26:12.14
delay 7 seconds @12:26:17.62 delay 4 seconds @12:26:19.17
CS.seek 3ml @12:26:21.65 ATV.dial ARV @12:26:22.18 delay 2 seconds @12:26:24.21 ARV.dial :carrier @12:26:24.22 delay 2 seconds @12:26:26.31 AS.seek 0.65ml @12:26:26.92
CSV.dial :waste @12:26:27.68 CS.seek 0ml @12:26:35.33 Disinfecting external Intake with mfbkill @12:26:35.47 CSV.dial CSR @12:26:36.85 Pulling 8ml of CSR.mfbkill @12:26:36.86 CSR.select :mfbkill @12:26:36.98 CS.seek 8ml @12:26:55.36 delay 5 seconds @12:27:00.39 CSR.select :air @12:27:00.52 CS.seek 10ml @12:27:06.06 CSV.dial CBV @12:27:06.10
CBV.dial CSV @12:27:07.12 CTV.dial :intake @12:27:07.87 Intake.select :open @12:27:07.99 CS.seek 2ml @12:27:16.98 delay 4 seconds @12:27:21.01 ATV.dial PCR @12:27:21.59 ASV.dial :waste1 @12:27:22.37 ARV.dial :air1 @12:27:22.76 Positioning Slug by 0.324ml @12:27:22.85 AS.seek 0.326ml @12:27:27.67
Intake.select :close @12:27:27.78 CTV.dial :air @12:27:28.55 CS.reconfigure CSconfig @12:27:28.61 CS.seek 0ml @12:27:34.73 Flushing lower manifold's waste1 port @12:27:34.74 CSV.dial CSR @12:27:35.83 Pulling 4ml of CSR.mfbkill @12:27:35.84 CSR.select :mfbkill @12:27:35.97 CS.seek 4ml @12:27:47.14 delay 5 seconds @12:27:52.17 CSR.select :air @12:27:52.30 CS.reconfigure CSfast @12:27:52.34 CS.seek 10ml @12:28:06.49 CSV.dial CTV @12:28:06.52
CTV.dial CSV @12:28:07.95 CBV.dial :waste1 @12:28:08.43 CS.seek 0ml @12:28:31.20 Flushing lower manifold's waste2b port @12:28:31.21 CSV.dial CSR @12:28:31.73 Pulling 4ml of CSR.mfbkill @12:28:31.74 CSR.select :mfbkill @12:28:31.87 CS.reconfigure CSconfig @12:28:31.92 CS.seek 4ml @12:28:43.17 delay 5 seconds @12:28:48.20 CSR.select :air @12:28:48.33 CS.reconfigure CSfast @12:28:48.37 CS.seek 10ml @12:29:02.52 CSV.dial CTV @12:29:02.55
CTV.dial CSV @12:29:03.03 CBV.dial :waste2b @12:29:03.82 CS.seek 0ml @12:29:26.46 Killing upper manifold @12:29:26.47 CSV.dial CSR @12:29:27.00 Pulling 4ml of CSR.mfbkill @12:29:27.01 CSR.select :mfbkill @12:29:27.13 CS.reconfigure CSconfig @12:29:27.78 CS.seek 4ml @12:29:38.99 delay 5 seconds @12:29:44.02 CSR.select :air @12:29:44.15 CS.reconfigure CSfast @12:29:44.19 CS.seek 10ml @12:29:58.21 CSV.dial CBV @12:29:58.24
CBV.dial CSV @12:29:59.33 CTV.dial :air @12:30:00.65 CS.seek 0ml @12:30:23.36 delay 7 seconds @12:30:30.40 CS.seek 3ml @12:30:37.96 CSV.dial :waste @12:30:38.44 CS.seek 0ml @12:30:46.08 Flushing upper manifold @12:30:46.22 CSV.dial :flush @12:30:46.69 CS.seek 5ml @12:30:58.55 delay 5 seconds @12:31:03.58 CSV.dial :air @12:31:04.12 CS.seek 7ml @12:31:09.71 CSV.dial CBV @12:31:09.75
CBV.dial CSV @12:31:10.79 CTV.dial :air @12:31:10.81 CS.seek 0ml @12:31:26.83 delay 7 seconds @12:31:33.87 CS.seek 3ml @12:31:41.58 CSV.dial :waste @12:31:42.05 CS.seek 0ml @12:31:49.77 CSV.dial :flush @12:31:50.25 CS.seek 3ml @12:31:57.80 delay 5 seconds @12:32:02.83 CSV.dial :air @12:32:03.36 CS.seek 5ml @12:32:08.83 CSV.dial :air @12:32:08.85 CS.seek 0ml @12:32:20.65 delay 7 seconds @12:32:27.69 CS.seek 3ml @12:32:35.21 CSV.dial :waste @12:32:35.95 CS.seek 0ml @12:32:43.52 Flushing lower manifold's waste1 port @12:32:43.66 CSV.dial :flush @12:32:44.14 CS.seek 5ml @12:32:48.89 delay 5 seconds @12:32:53.92 ATV.dial ARV @12:32:54.67 ASV.dial :PCRlock @12:32:55.03 delay 5 seconds @12:32:55.99
delay 5 seconds @12:33:00.06 Running OM Profile60C350 thermocycling @12:33:00.08 PCR.program Profile60C350 @12:33:00.19 Profile60C350 should complete in < 2:02:45 @12:33:00.20 PCR.run 2:38:26.25 @12:33:01.01
CSV.dial :air @12:33:01.53 CS.seek 7ml @12:33:07.02 CSV.dial CTV @12:33:07.05
CTV.dial CSV @12:33:08.46 CBV.dial :waste1 @12:33:08.95 CS.seek 0ml @12:33:25.18 Flushing lower manifold's waste2b port @12:33:25.32 CSV.dial :flush @12:33:26.10 CS.seek 5ml @12:33:38.02 delay 5 seconds @12:33:43.04 CSV.dial :air @12:33:43.58 CS.seek 7ml @12:33:48.99 CSV.dial CTV @12:33:49.02
CTV.dial CSV @12:33:49.49 CBV.dial :waste2b @12:33:50.30 CS.seek 0ml @12:34:06.37 Disinfecting external Intake with kill @12:34:06.51 CSV.dial CSR @12:34:07.03 Pulling 8ml of CSR.kill @12:34:07.04 CSR.select :kill @12:34:07.16 CS.seek 8ml @12:34:25.56 delay 5 seconds @12:34:30.59 CSR.select :air @12:34:30.72 CS.seek 10ml @12:34:36.20 CSV.dial CBV @12:34:36.22
CBV.dial CSV @12:34:37.32 CTV.dial :intake @12:34:38.09 Intake.select :open @12:34:38.21 CS.seek 2ml @12:34:57.62 Intake.select :close @12:34:57.73 CTV.dial :air @12:34:58.49 CS.reconfigure CSconfig @12:34:58.54 CS.seek 0ml @12:35:04.68 CSV.dial CSR @12:35:05.77 Pulling 5ml of CSR.flush @12:35:05.78 CSR.select :flush @12:35:05.91 CS.seek 5ml @12:35:19.65 delay 5 seconds @12:35:24.68 CSR.select :air @12:35:24.81 CS.seek 10ml @12:35:38.49 CSV.dial CBV @12:35:39.54 CS.seek 0ml @12:36:06.06 Garaging FlushPuck in Collection Clamp @12:36:06.14 Elbow.reconfigure TightElbow @12:36:06.19 Elbow.seek CC @12:36:06.46 Elbow.reconfigure LooseElbow @12:36:06.56 Forearm.seek CC @12:36:07.53 Hand.close @12:36:08.16 CC.seek :open @12:36:12.53 Forearm.seek :retract @12:36:13.57 Elbow.reconfigure TightElbow @12:36:13.62 Elbow.seek :garage @12:36:16.81 Elbow.reconfigure LooseElbow @12:36:16.93 Forearm.reconfigure TightForearm @12:36:16.98 Forearm.seek :garage @12:36:18.11 Hand.open @12:36:18.85 Forearm.reconfigure LooseForearm @12:36:18.90 Forearm.seek :retract @12:36:19.89 Starting initialPurge @12:36:19.90 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:36:19.98 Hand.open @12:36:20.35 Elbow.reconfigure TightElbow @12:36:20.40 Elbow.seek FlushPuck::Garage @12:36:20.68 Elbow.reconfigure LooseElbow @12:36:20.78 Forearm.seek :garage @12:36:21.78 Hand.close @12:36:22.55 Forearm.seek :retract @12:36:23.57 Elbow.reconfigure TightElbow @12:36:23.62 Elbow.seek CC @12:36:26.58 Elbow.reconfigure LooseElbow @12:36:26.69 Forearm.reconfigure TightForearm @12:36:26.74 Forearm.seek CC @12:36:28.69 CC.seek :closed @12:36:32.95 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.88 @12:36:33.03 Hand.open @12:36:33.74 Forearm.reconfigure LooseForearm @12:36:33.79 Forearm.seek :retract @12:36:34.78 Flushing the Collection Valve::Manifold @12:36:34.92 CSV.dial CSR @12:36:36.02 Pulling 5ml of CSR.flush @12:36:36.03 CSR.select :flush @12:36:36.16 CS.seek 5ml @12:36:50.12 delay 5 seconds @12:36:55.14 CSR.select :air @12:36:55.31 CS.reconfigure CSfast @12:36:55.36 CS.seek 7.5ml @12:37:01.93 CSV.dial :waste @12:37:03.23 CS.reconfigure CSconfig @12:37:03.28 CS.seek 0ml @12:37:23.53 Purging external Intake @12:37:23.66 CSV.dial :flush @12:37:24.12 CS.reconfigure CSfast @12:37:24.17 CS.seek 10ml @12:37:46.59 delay 5 seconds @12:37:51.61 CSV.dial CBV @12:37:51.65
CBV.dial CSV @12:37:52.42 CTV.dial :intake @12:37:53.20 Intake.select :open @12:37:53.32 CS.seek 0ml @12:38:17.44 Intake.select :close @12:38:17.55 CTV.dial :air @12:38:18.33 Flushing upper manifold @12:38:18.46 CSV.dial :flush @12:38:19.21 CS.seek 5ml @12:38:31.09 delay 5 seconds @12:38:36.11 CSV.dial :air @12:38:36.65 CS.seek 7ml @12:38:42.28 CSV.dial CBV @12:38:42.30
CBV.dial CSV @12:38:43.36 CTV.dial :air @12:38:43.38 CS.seek 0ml @12:38:59.50 delay 7 seconds @12:39:06.53 CS.seek 3ml @12:39:14.06 CSV.dial :waste @12:39:14.52 CS.seek 0ml @12:39:22.21 CSV.dial :flush @12:39:22.68 CS.seek 3ml @12:39:30.19 delay 5 seconds @12:39:35.21 CSV.dial :air @12:39:35.73 CS.seek 5ml @12:39:41.16 CSV.dial :air @12:39:41.18 CS.seek 0ml @12:39:53.06 delay 7 seconds @12:40:00.09 CS.seek 3ml @12:40:07.72 CSV.dial :waste @12:40:09.10 CS.seek 0ml @12:40:16.75 Garaging FlushPuck in Collection Clamp @12:40:16.83 Elbow.reconfigure TightElbow @12:40:16.88 Elbow.seek CC @12:40:17.13 Elbow.reconfigure LooseElbow @12:40:17.24 Forearm.seek CC @12:40:18.23 Hand.close @12:40:18.80 CC.seek :open @12:40:23.16 Forearm.seek :retract @12:40:24.15 Elbow.reconfigure TightElbow @12:40:24.20 Elbow.seek :garage @12:40:27.40 Elbow.reconfigure LooseElbow @12:40:27.53 Forearm.reconfigure TightForearm @12:40:27.58 Forearm.seek :garage @12:40:28.71 Hand.open @12:40:29.36 Forearm.reconfigure LooseForearm @12:40:29.41 Forearm.seek :retract @12:40:30.43 Completed [stdphase] begun 10:00:47 @12:40:30.44 Preparing to sleep until Sun May 17 10:00:47 +0200 2020 @12:40:30.50 deferred until MFBPCR finish @12:40:30.52
Waiting for MFBPCR til 10:00CEST17-May-20 @12:40:30.54 Continuing at 10:00CEST17-May-20 @12:40:30.60 GoodNews.email "Then, in tube 2: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:124\n{1 => 12, 2 => 10}\nCan@12:40:30, 8.4C, 43% humidity, 14.3psia, 14.114V, 0.493A, 0.487A avg, 6.96W",Subject: "Waiting until 10:00CEST17-May-20" @14:09:36.81 PCR.off @14:09:38.07 PCR for OM completed -- closed /var/log/esp/OM20may1612h625ml.pcr @14:09:38.14 Cleaning PCR @14:09:38.15 AFV.select :carrierSolution @14:09:38.26 delay 2 seconds @14:09:40.29 AS.reconfigure ASconfig @14:09:40.34 AS.seek 0.5ml @14:09:50.65 delay 1 second @14:09:51.67 AFV.select :holdingCoil @14:09:51.77 ASV.dial :waste1 @14:09:52.15 ATV.dial :air3 @14:09:53.39 AS.reconfigure ASslow1 @14:09:53.44 AS.seek 0.51ml @14:09:56.33 delay 2 seconds @14:09:58.36 ATV.dial :bleach @14:09:58.97 AS.seek 0.81ml @14:10:59.54 delay 3 seconds @14:11:02.57 ATV.dial :air3 @14:11:03.10 delay 3 seconds @14:11:06.19 AS.seek 0.835ml @14:11:11.48 delay 3 seconds @14:11:14.51 ATV.dial :bleach @14:11:15.12 AS.seek 0.82ml @14:11:18.41 delay 3 seconds @14:11:21.44 ARV.dial :waste @14:11:22.02 delay 3 seconds @14:11:25.05 ATV.dial :ARV @14:11:25.91 AS.reconfigure ASconfig @14:11:25.96 AS.seek 0.47ml @14:11:46.37 AFV.select :carrierSolution @14:11:46.48 delay 2 seconds @14:11:48.52 AS.seek 1ml @14:12:19.34 delay 1 second @14:12:20.36 AFV.select :holdingCoil @14:12:20.46 delay 1 second @14:12:21.49 AS.reconfigure ASfast @14:12:21.54 AS.seek 0ml @14:12:48.88 AFV.select :carrierSolution @14:12:48.99 delay 2 seconds @14:12:51.03 AS.reconfigure ASconfig @14:12:51.08 AS.seek 1ml @14:13:48.82 delay 1 second @14:13:49.84 AFV.select :holdingCoil @14:13:49.94 delay 1 second @14:13:50.97 AS.reconfigure ASfast @14:13:51.02 AS.seek 0ml @14:14:18.34 AFV.select :carrierSolution @14:14:18.45 delay 2 seconds @14:14:20.48 AS.reconfigure ASconfig @14:14:20.53 AS.seek 1ml @14:15:18.29 delay 1 second @14:15:19.31 AFV.select :holdingCoil @14:15:19.41 delay 1 second @14:15:20.44 AS.reconfigure ASfast @14:15:20.49 AS.seek 0ml @14:15:47.79 ARV.dial between carrier and air1 @14:15:48.28 ATV.dial :air3 @14:15:49.52 AS.reconfigure ASslow1 @14:15:49.57 AS.seek 0.01ml @14:15:51.84 delay 2 seconds @14:15:53.86 ATV.dial :bleach @14:15:54.47 AS.seek 0.81ml @14:18:35.61 delay 3 seconds @14:18:38.63 ATV.dial :air3 @14:18:39.17 delay 3 seconds @14:18:42.26 AS.seek 0.835ml @14:18:47.54 delay 3 seconds @14:18:50.57 ATV.dial :bleach @14:18:51.18 AS.seek 0.82ml @14:18:54.47 delay 3 seconds @14:18:57.49 ATV.dial PCR @14:18:57.89 AS.reconfigure ASconfig @14:18:57.94 AS.seek :empty @14:19:45.40 PCR.program :bleachProfile @14:19:45.46 PCR program should complete in < 2:45 @14:19:45.47 PCR.run 10:00 @14:23:02.44 PCR.off @14:23:02.47 AFV.select :carrierSolution @14:23:02.58 delay 2 seconds @14:23:04.62 AS.seek 1ml @14:24:02.42 delay 1 second @14:24:03.44 AFV.select :holdingCoil @14:24:03.54 delay 1 second @14:24:04.57 AS.reconfigure ASfast @14:24:04.62 AS.seek 0ml @14:24:31.94 delay 3 seconds @14:24:34.97 AFV.select :carrierSolution @14:24:35.07 delay 2 seconds @14:24:37.10 AS.reconfigure ASconfig @14:24:37.15 AS.seek 1ml @14:25:34.99 delay 1 second @14:25:36.01 AFV.select :holdingCoil @14:25:36.11 delay 1 second @14:25:37.14 AS.reconfigure ASfast @14:25:37.19 AS.seek 0ml @14:26:04.52 delay 3 seconds @14:26:07.55 AFV.select :carrierSolution @14:26:07.65 delay 2 seconds @14:26:09.68 AS.reconfigure ASconfig @14:26:09.74 AS.seek 1ml @14:27:07.50 delay 1 second @14:27:08.52 AFV.select :holdingCoil @14:27:08.62 delay 1 second @14:27:10.22 AS.reconfigure ASfast @14:27:10.31 AS.seek 0ml @14:27:37.63 delay 3 seconds @14:27:40.66 AFV.select :carrierSolution @14:27:40.76 delay 2 seconds @14:27:42.79 AS.reconfigure ASconfig @14:27:42.85 AS.seek 1ml @14:28:40.69 delay 1 second @14:28:41.71 AFV.select :holdingCoil @14:28:41.81 delay 1 second @14:28:42.84 AS.reconfigure ASfast @14:28:42.89 AS.seek 0ml @14:29:10.19 delay 3 seconds @14:29:13.22 AFV.select :carrierSolution @14:29:13.32 delay 2 seconds @14:29:15.35 AS.reconfigure ASconfig @14:29:15.41 AS.seek 1ml @14:30:13.19 delay 1 second @14:30:14.21 AFV.select :holdingCoil @14:30:14.31 delay 1 second @14:30:15.34 AS.reconfigure ASnorm @14:30:15.39 AS.seek 0ml @14:31:06.12 Setting up PCR for ML -- writing /var/log/esp/ML20may1614h625ml.pcr @14:31:06.14 Building PCR Slug from ASV with MM2 mix, PP4 primer @14:31:06.16 Priming with carrier @14:31:06.17 ATV.dial ARV @14:31:06.76 ARV.dial :carrier @14:31:07.11 AS.seek 0.5ml @14:31:32.69 AFV.select :carrierSolution @14:31:32.80 delay 2 seconds @14:31:34.83 AS.reconfigure ASconfig @14:31:34.88 AS.seek 1ml @14:32:03.94 delay 1 second @14:32:04.96 AFV.select :holdingCoil @14:32:05.06 delay 1 second @14:32:06.09 ATV.dial :waste @14:32:07.66 AS.reconfigure ASnorm @14:32:07.72 AS.seek :empty @14:32:58.45 AFV.select :carrierSolution @14:32:58.56 delay 2 seconds @14:33:00.59 AS.reconfigure ASconfig @14:33:00.64 AS.seek 0.5ml @14:33:29.74 delay 1 second @14:33:30.76 AFV.select :holdingCoil @14:33:30.86 Building Slug @14:33:30.87 ATV.dial :ARV @14:33:32.43 delay 2 seconds @14:33:34.46 ARV.dial :air1 @14:33:34.85 delay 2 seconds @14:33:36.94 AS.reconfigure ASslow2 @14:33:36.99 AS.seek 0.506ml @14:33:43.21 delay 2 seconds @14:33:45.23 ARV.dial :MM2 @14:33:46.63 delay 2 seconds @14:33:48.66 Adding MM2 @14:33:48.73 AS.seek 0.524ml @14:34:07.01 delay 25 seconds @14:34:32.04 ARV.dial :PP4 @14:34:32.43 Adding PP4 @14:34:32.45 delay 2 seconds @14:34:34.53 AS.seek 0.53ml @14:34:40.71 delay 10 seconds @14:34:50.74 ARV.dial :air3 @14:34:51.12 adding ARV air @14:34:51.14 delay 2 seconds @14:34:53.22 AS.seek 0.536ml @14:34:59.43 delay 5 seconds @14:35:04.46 ARV.dial :carrier @14:35:05.67 delay 7 seconds @14:35:12.76 AS.seek 0.594ml @14:36:11.23 delay 5 seconds @14:36:16.26 ATV.dial :ASV @14:36:16.65 delay 2 seconds @14:36:18.74 AS.seek 0.6ml @14:36:24.96 delay 4 seconds @14:36:28.99 ATV.dial ARV @14:36:29.62 delay 2 seconds @14:36:31.65 ARV.dial :carrier @14:36:31.66 delay 2 seconds @14:36:33.75 AS.seek 0.65ml @14:37:24.34 delay 4 seconds @14:37:28.37 ATV.dial PCR @14:37:28.96 ASV.dial :waste1 @14:37:28.97 ARV.dial :air1 @14:37:29.35 Positioning Slug by 0.324ml @14:37:29.44 AS.seek 0.326ml @14:42:55.52 delay 5 seconds @14:43:00.55 ATV.dial ARV @14:43:01.30 ASV.dial :PCRlock @14:43:01.69 delay 5 seconds @14:43:06.72 Running ML Profile60C350 thermocycling @14:43:06.74 PCR.program Profile60C350 @14:43:06.85 Profile60C350 should complete in < 2:02:45 @14:43:06.86 PCR.run 2:38:26.25 @16:19:32.52 PCR.off @16:19:33.78 PCR for ML completed -- closed /var/log/esp/ML20may1614h625ml.pcr @16:19:33.85 Cleaning PCR @16:19:33.86 AFV.select :carrierSolution @16:19:33.97 delay 2 seconds @16:19:36.00 AS.reconfigure ASconfig @16:19:36.05 AS.seek 0.5ml @16:19:46.34 delay 1 second @16:19:47.36 AFV.select :holdingCoil @16:19:47.46 ASV.dial :waste1 @16:19:47.84 ATV.dial :air3 @16:19:49.08 AS.reconfigure ASslow1 @16:19:49.13 AS.seek 0.51ml @16:19:51.39 delay 2 seconds @16:19:53.42 ATV.dial :bleach @16:19:54.02 AS.seek 0.81ml @16:20:54.59 delay 3 seconds @16:20:57.62 ATV.dial :air3 @16:20:58.15 delay 3 seconds @16:21:01.24 AS.seek 0.835ml @16:21:06.53 delay 3 seconds @16:21:09.55 ATV.dial :bleach @16:21:10.16 AS.seek 0.82ml @16:21:13.45 delay 3 seconds @16:21:16.48 ARV.dial :waste @16:21:17.06 delay 3 seconds @16:21:20.09 ATV.dial :ARV @16:21:20.94 AS.reconfigure ASconfig @16:21:20.99 AS.seek 0.47ml @16:21:41.40 AFV.select :carrierSolution @16:21:41.51 delay 2 seconds @16:21:43.55 AS.seek 1ml @16:22:14.31 delay 1 second @16:22:15.33 AFV.select :holdingCoil @16:22:15.43 delay 1 second @16:22:16.46 AS.reconfigure ASfast @16:22:16.51 AS.seek 0ml @16:22:43.84 AFV.select :carrierSolution @16:22:43.95 delay 2 seconds @16:22:46.58 AS.reconfigure ASconfig @16:22:46.65 AS.seek 1ml @16:23:44.45 delay 1 second @16:23:45.47 AFV.select :holdingCoil @16:23:45.57 delay 1 second @16:23:46.60 AS.reconfigure ASfast @16:23:46.65 AS.seek 0ml @16:24:14.00 AFV.select :carrierSolution @16:24:14.11 delay 2 seconds @16:24:16.14 AS.reconfigure ASconfig @16:24:16.19 AS.seek 1ml @16:25:13.98 delay 1 second @16:25:15.00 AFV.select :holdingCoil @16:25:15.10 delay 1 second @16:25:16.13 AS.reconfigure ASfast @16:25:16.18 AS.seek 0ml @16:25:43.52 ARV.dial between carrier and air1 @16:25:44.00 ATV.dial :air3 @16:25:45.24 AS.reconfigure ASslow1 @16:25:45.29 AS.seek 0.01ml @16:25:47.58 delay 2 seconds @16:25:49.60 ATV.dial :bleach @16:25:50.21 AS.seek 0.81ml @16:28:31.38 delay 3 seconds @16:28:34.41 ATV.dial :air3 @16:28:34.94 delay 3 seconds @16:28:38.03 AS.seek 0.835ml @16:28:43.42 delay 3 seconds @16:28:46.45 ATV.dial :bleach @16:28:47.06 AS.seek 0.82ml @16:28:50.35 delay 3 seconds @16:28:53.38 ATV.dial PCR @16:28:53.78 AS.reconfigure ASconfig @16:28:53.83 AS.seek :empty @16:29:41.30 PCR.program :bleachProfile @16:29:41.35 PCR program should complete in < 2:45 @16:29:41.36 PCR.run 10:00 @16:32:58.33 PCR.off @16:32:58.36 AFV.select :carrierSolution @16:32:58.47 delay 2 seconds @16:33:00.51 AS.seek 1ml @16:33:58.37 delay 1 second @16:33:59.39 AFV.select :holdingCoil @16:33:59.49 delay 1 second @16:34:00.52 AS.reconfigure ASfast @16:34:00.57 AS.seek 0ml @16:34:27.94 delay 3 seconds @16:34:30.97 AFV.select :carrierSolution @16:34:31.07 delay 2 seconds @16:34:33.10 AS.reconfigure ASconfig @16:34:33.16 AS.seek 1ml @16:35:30.93 delay 1 second @16:35:31.95 AFV.select :holdingCoil @16:35:32.05 delay 1 second @16:35:33.08 AS.reconfigure ASfast @16:35:33.13 AS.seek 0ml @16:36:00.48 delay 3 seconds @16:36:03.51 AFV.select :carrierSolution @16:36:03.61 delay 2 seconds @16:36:05.64 AS.reconfigure ASconfig @16:36:05.70 AS.seek 1ml @16:37:03.46 delay 1 second @16:37:04.48 AFV.select :holdingCoil @16:37:04.58 delay 1 second @16:37:05.61 AS.reconfigure ASfast @16:37:05.66 AS.seek 0ml @16:37:32.99 delay 3 seconds @16:37:36.02 AFV.select :carrierSolution @16:37:36.12 delay 2 seconds @16:37:38.15 AS.reconfigure ASconfig @16:37:38.20 AS.seek 1ml @16:38:36.01 delay 1 second @16:38:37.03 AFV.select :holdingCoil @16:38:37.13 delay 1 second @16:38:38.16 AS.reconfigure ASfast @16:38:38.21 AS.seek 0ml @16:39:05.57 delay 3 seconds @16:39:08.60 AFV.select :carrierSolution @16:39:08.70 delay 2 seconds @16:39:10.73 AS.reconfigure ASconfig @16:39:10.79 AS.seek 1ml @16:40:08.59 delay 1 second @16:40:09.61 AFV.select :holdingCoil @16:40:09.71 delay 1 second @16:40:10.74 AS.reconfigure ASnorm @16:40:10.79 AS.seek 0ml @16:41:01.55 Setting up PCR for CG -- writing /var/log/esp/CG20may1616h625ml.pcr @16:41:01.56 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:41:01.58 Priming with carrier @16:41:01.59 ATV.dial ARV @16:41:02.17 ARV.dial :carrier @16:41:02.52 AS.seek 0.5ml @16:41:28.05 AFV.select :carrierSolution @16:41:28.17 delay 2 seconds @16:41:30.20 AS.reconfigure ASconfig @16:41:30.25 AS.seek 1ml @16:41:59.28 delay 1 second @16:42:00.30 AFV.select :holdingCoil @16:42:00.40 delay 1 second @16:42:02.05 ATV.dial :waste @16:42:03.62 AS.reconfigure ASnorm @16:42:03.68 AS.seek :empty @16:42:54.42 AFV.select :carrierSolution @16:42:54.53 delay 2 seconds @16:42:56.56 AS.reconfigure ASconfig @16:42:56.62 AS.seek 0.5ml @16:43:25.70 delay 1 second @16:43:26.72 AFV.select :holdingCoil @16:43:26.82 Building Slug @16:43:26.83 ATV.dial :ARV @16:43:28.39 delay 2 seconds @16:43:30.42 ARV.dial :air1 @16:43:30.80 delay 2 seconds @16:43:32.89 AS.reconfigure ASslow2 @16:43:32.94 AS.seek 0.506ml @16:43:39.36 delay 2 seconds @16:43:41.38 ARV.dial :MM1 @16:43:41.95 delay 2 seconds @16:43:43.98 Adding MM1 @16:43:44.05 AS.seek 0.524ml @16:44:02.51 delay 25 seconds @16:44:27.54 ARV.dial :PP2 @16:44:27.92 Adding PP2 @16:44:27.94 delay 2 seconds @16:44:30.02 AS.seek 0.53ml @16:44:36.44 delay 10 seconds @16:44:46.47 ARV.dial :air2 @16:44:46.86 adding ARV air @16:44:46.88 delay 2 seconds @16:44:48.96 AS.seek 0.536ml @16:44:55.17 delay 5 seconds @16:45:00.20 ARV.dial :carrier @16:45:01.41 delay 7 seconds @16:45:08.50 AS.seek 0.594ml @16:46:06.95 delay 5 seconds @16:46:11.98 ATV.dial :ASV @16:46:12.37 delay 2 seconds @16:46:14.46 AS.seek 0.6ml @16:46:20.69 delay 4 seconds @16:46:24.72 ATV.dial ARV @16:46:25.27 delay 2 seconds @16:46:27.30 ARV.dial :carrier @16:46:27.31 delay 2 seconds @16:46:29.40 AS.seek 0.65ml @16:47:19.84 delay 4 seconds @16:47:23.87 ATV.dial PCR @16:47:24.45 ASV.dial :waste1 @16:47:24.46 ARV.dial :air1 @16:47:24.85 Positioning Slug by 0.324ml @16:47:24.94 AS.seek 0.326ml @16:52:51.18 delay 5 seconds @16:52:56.21 ATV.dial ARV @16:52:57.05 ASV.dial :PCRlock @16:52:57.44 delay 5 seconds @16:53:02.47 Running CG Profile60C300 thermocycling @16:53:02.49 PCR.program Profile60C300 @16:53:02.59 Profile60C300 should complete in < 1:59:25 @16:53:02.60 PCR.run 2:34:16.25 @18:28:37.43 PCR.off @18:28:38.10 PCR for CG completed -- closed /var/log/esp/CG20may1616h625ml.pcr @18:28:38.17 Cleaning PCR @18:28:38.18 AFV.select :carrierSolution @18:28:38.29 delay 2 seconds @18:28:40.32 AS.reconfigure ASconfig @18:28:40.37 AS.seek 0.5ml @18:28:50.67 delay 1 second @18:28:51.69 AFV.select :holdingCoil @18:28:51.79 ASV.dial :waste1 @18:28:52.17 ATV.dial :air3 @18:28:53.40 AS.reconfigure ASslow1 @18:28:53.45 AS.seek 0.51ml @18:28:55.74 delay 2 seconds @18:28:57.76 ATV.dial :bleach @18:28:58.37 AS.seek 0.81ml @18:29:58.95 delay 3 seconds @18:30:01.98 ATV.dial :air3 @18:30:02.52 delay 3 seconds @18:30:05.61 AS.seek 0.835ml @18:30:10.89 delay 3 seconds @18:30:13.92 ATV.dial :bleach @18:30:14.52 AS.seek 0.82ml @18:30:17.83 delay 3 seconds @18:30:20.86 ARV.dial :waste @18:30:21.44 delay 3 seconds @18:30:24.47 ATV.dial :ARV @18:30:25.31 AS.reconfigure ASconfig @18:30:25.37 AS.seek 0.47ml @18:30:45.77 AFV.select :carrierSolution @18:30:45.88 delay 2 seconds @18:30:47.92 AS.seek 1ml @18:31:18.70 delay 1 second @18:31:19.72 AFV.select :holdingCoil @18:31:19.82 delay 1 second @18:31:20.85 AS.reconfigure ASfast @18:31:20.90 AS.seek 0ml @18:31:48.22 AFV.select :carrierSolution @18:31:48.33 delay 2 seconds @18:31:50.36 AS.reconfigure ASconfig @18:31:50.41 AS.seek 1ml @18:32:48.17 delay 1 second @18:32:49.19 AFV.select :holdingCoil @18:32:49.29 delay 1 second @18:32:50.32 AS.reconfigure ASfast @18:32:50.37 AS.seek 0ml @18:33:17.69 AFV.select :carrierSolution @18:33:17.80 delay 2 seconds @18:33:19.83 AS.reconfigure ASconfig @18:33:19.88 AS.seek 1ml @18:34:17.69 delay 1 second @18:34:18.71 AFV.select :holdingCoil @18:34:18.81 delay 1 second @18:34:19.84 AS.reconfigure ASfast @18:34:19.89 AS.seek 0ml @18:34:47.22 ARV.dial between carrier and air1 @18:34:47.69 ATV.dial :air3 @18:34:48.93 AS.reconfigure ASslow1 @18:34:48.98 AS.seek 0.01ml @18:34:51.27 delay 2 seconds @18:34:53.29 ATV.dial :bleach @18:34:53.90 AS.seek 0.81ml @18:37:35.04 delay 3 seconds @18:37:38.07 ATV.dial :air3 @18:37:38.60 delay 3 seconds @18:37:41.69 AS.seek 0.835ml @18:37:46.97 delay 3 seconds @18:37:50.00 ATV.dial :bleach @18:37:50.62 AS.seek 0.82ml @18:37:53.91 delay 3 seconds @18:37:56.93 ATV.dial PCR @18:37:57.32 AS.reconfigure ASconfig @18:37:57.37 AS.seek :empty @18:38:44.82 PCR.program :bleachProfile @18:38:44.87 PCR program should complete in < 2:45 @18:38:44.88 PCR.run 10:00 @18:41:59.44 PCR.off @18:41:59.47 AFV.select :carrierSolution @18:41:59.57 delay 2 seconds @18:42:01.61 AS.seek 1ml @18:42:59.45 delay 1 second @18:43:00.47 AFV.select :holdingCoil @18:43:00.57 delay 1 second @18:43:01.60 AS.reconfigure ASfast @18:43:01.65 AS.seek 0ml @18:43:28.98 delay 3 seconds @18:43:32.01 AFV.select :carrierSolution @18:43:32.11 delay 2 seconds @18:43:34.14 AS.reconfigure ASconfig @18:43:34.20 AS.seek 1ml @18:44:31.98 delay 1 second @18:44:33.00 AFV.select :holdingCoil @18:44:33.10 delay 1 second @18:44:34.13 AS.reconfigure ASfast @18:44:34.18 AS.seek 0ml @18:45:01.51 delay 3 seconds @18:45:04.54 AFV.select :carrierSolution @18:45:04.64 delay 2 seconds @18:45:06.67 AS.reconfigure ASconfig @18:45:06.73 AS.seek 1ml @18:46:04.51 delay 1 second @18:46:05.53 AFV.select :holdingCoil @18:46:05.63 delay 1 second @18:46:06.66 AS.reconfigure ASfast @18:46:06.71 AS.seek 0ml @18:46:34.03 delay 3 seconds @18:46:37.06 AFV.select :carrierSolution @18:46:37.16 delay 2 seconds @18:46:39.19 AS.reconfigure ASconfig @18:46:39.25 AS.seek 1ml @18:47:36.98 delay 1 second @18:47:38.00 AFV.select :holdingCoil @18:47:38.10 delay 1 second @18:47:39.13 AS.reconfigure ASfast @18:47:39.18 AS.seek 0ml @18:48:06.50 delay 3 seconds @18:48:09.53 AFV.select :carrierSolution @18:48:09.63 delay 2 seconds @18:48:11.66 AS.reconfigure ASconfig @18:48:11.72 AS.seek 1ml @18:49:09.47 delay 1 second @18:49:10.49 AFV.select :holdingCoil @18:49:10.59 delay 1 second @18:49:11.62 AS.reconfigure ASnorm @18:49:11.67 AS.seek 0ml @18:50:02.41 Setting up PCR for NM -- writing /var/log/esp/NM20may1618h625ml.pcr @18:50:02.42 Building PCR Slug from ASV with MM1 mix, PP1 primer @18:50:02.44 Priming with carrier @18:50:02.45 ATV.dial ARV @18:50:03.03 ARV.dial :carrier @18:50:03.38 AS.seek 0.5ml @18:50:28.88 AFV.select :carrierSolution @18:50:29.00 delay 2 seconds @18:50:31.03 AS.reconfigure ASconfig @18:50:31.08 AS.seek 1ml @18:51:00.13 delay 1 second @18:51:01.15 AFV.select :holdingCoil @18:51:01.25 delay 1 second @18:51:02.28 ATV.dial :waste @18:51:03.84 AS.reconfigure ASnorm @18:51:03.89 AS.seek :empty @18:51:54.63 AFV.select :carrierSolution @18:51:54.74 delay 2 seconds @18:51:56.77 AS.reconfigure ASconfig @18:51:56.82 AS.seek 0.5ml @18:52:25.97 delay 1 second @18:52:26.99 AFV.select :holdingCoil @18:52:27.09 Building Slug @18:52:27.10 ATV.dial :ARV @18:52:28.66 delay 2 seconds @18:52:30.69 ARV.dial :air1 @18:52:31.07 delay 2 seconds @18:52:33.15 AS.reconfigure ASslow2 @18:52:33.21 AS.seek 0.506ml @18:52:39.53 delay 2 seconds @18:52:41.55 ARV.dial :MM1 @18:52:42.11 delay 2 seconds @18:52:44.14 Adding MM1 @18:52:44.21 AS.seek 0.524ml @18:53:02.58 delay 25 seconds @18:53:27.61 ARV.dial :PP1 @18:53:27.99 Adding PP1 @18:53:28.01 delay 2 seconds @18:53:30.09 AS.seek 0.53ml @18:53:36.33 delay 10 seconds @18:53:46.36 ARV.dial :air1 @18:53:46.74 adding ARV air @18:53:46.75 delay 2 seconds @18:53:48.84 AS.seek 0.536ml @18:53:55.00 delay 5 seconds @18:54:00.03 ARV.dial :carrier @18:54:00.41 delay 7 seconds @18:54:07.50 AS.seek 0.594ml @18:55:06.11 delay 5 seconds @18:55:11.14 ATV.dial :ASV @18:55:12.16 delay 2 seconds @18:55:14.25 AS.seek 0.6ml @18:55:20.74 delay 4 seconds @18:55:24.77 ATV.dial ARV @18:55:25.41 delay 2 seconds @18:55:27.44 ARV.dial :carrier @18:55:27.45 delay 2 seconds @18:55:29.54 AS.seek 0.65ml @18:56:20.18 delay 4 seconds @18:56:24.21 ATV.dial PCR @18:56:24.82 ASV.dial :waste1 @18:56:24.83 ARV.dial :air1 @18:56:25.23 Positioning Slug by 0.324ml @18:56:25.32 AS.seek 0.326ml @19:01:51.43 delay 5 seconds @19:01:56.46 ATV.dial ARV @19:01:57.21 ASV.dial :PCRlock @19:01:57.60 delay 5 seconds @19:02:02.63 Running NM Profile60C300 thermocycling @19:02:02.65 PCR.program Profile60C300 @19:02:02.75 Profile60C300 should complete in < 1:59:25 @19:02:02.76 PCR.run 2:34:16.25 @20:37:31.10 PCR.off @20:37:31.76 PCR for NM completed -- closed /var/log/esp/NM20may1618h625ml.pcr @20:37:31.83 Cleaning PCR @20:37:31.84 AFV.select :carrierSolution @20:37:31.95 delay 2 seconds @20:37:33.98 AS.reconfigure ASconfig @20:37:34.03 AS.seek 0.5ml @20:37:44.33 delay 1 second @20:37:45.35 AFV.select :holdingCoil @20:37:45.45 ASV.dial :waste1 @20:37:45.83 ATV.dial :air3 @20:37:47.06 AS.reconfigure ASslow1 @20:37:47.12 AS.seek 0.51ml @20:37:49.42 delay 2 seconds @20:37:51.44 ATV.dial :bleach @20:37:52.04 AS.seek 0.81ml @20:38:52.63 delay 3 seconds @20:38:55.66 ATV.dial :air3 @20:38:56.19 delay 3 seconds @20:38:59.28 AS.seek 0.835ml @20:39:04.55 delay 3 seconds @20:39:07.57 ATV.dial :bleach @20:39:08.81 AS.seek 0.82ml @20:39:12.10 delay 3 seconds @20:39:15.13 ARV.dial :waste @20:39:15.71 delay 3 seconds @20:39:18.74 ATV.dial :ARV @20:39:19.59 AS.reconfigure ASconfig @20:39:19.64 AS.seek 0.47ml @20:39:40.04 AFV.select :carrierSolution @20:39:40.15 delay 2 seconds @20:39:42.19 AS.seek 1ml @20:40:12.94 delay 1 second @20:40:13.96 AFV.select :holdingCoil @20:40:14.06 delay 1 second @20:40:15.09 AS.reconfigure ASfast @20:40:15.14 AS.seek 0ml @20:40:42.47 AFV.select :carrierSolution @20:40:42.58 delay 2 seconds @20:40:44.61 AS.reconfigure ASconfig @20:40:44.66 AS.seek 1ml @20:41:42.46 delay 1 second @20:41:43.48 AFV.select :holdingCoil @20:41:43.58 delay 1 second @20:41:44.61 AS.reconfigure ASfast @20:41:44.66 AS.seek 0ml @20:42:11.99 AFV.select :carrierSolution @20:42:12.10 delay 2 seconds @20:42:14.13 AS.reconfigure ASconfig @20:42:14.18 AS.seek 1ml @20:43:12.01 delay 1 second @20:43:13.03 AFV.select :holdingCoil @20:43:13.13 delay 1 second @20:43:14.16 AS.reconfigure ASfast @20:43:14.21 AS.seek 0ml @20:43:41.54 ARV.dial between carrier and air1 @20:43:42.02 ATV.dial :air3 @20:43:43.27 AS.reconfigure ASslow1 @20:43:43.32 AS.seek 0.01ml @20:43:45.66 delay 2 seconds @20:43:47.68 ATV.dial :bleach @20:43:48.28 AS.seek 0.81ml @20:46:29.40 delay 3 seconds @20:46:32.43 ATV.dial :air3 @20:46:32.96 delay 3 seconds @20:46:36.05 AS.seek 0.835ml @20:46:41.35 delay 3 seconds @20:46:44.38 ATV.dial :bleach @20:46:44.99 AS.seek 0.82ml @20:46:48.29 delay 3 seconds @20:46:51.32 ATV.dial PCR @20:46:51.71 AS.reconfigure ASconfig @20:46:51.77 AS.seek :empty @20:47:39.23 PCR.program :bleachProfile @20:47:39.28 PCR program should complete in < 2:45 @20:47:39.30 PCR.run 10:00 @20:50:56.26 PCR.off @20:50:56.29 AFV.select :carrierSolution @20:50:56.40 delay 2 seconds @20:50:58.44 AS.seek 1ml @20:51:56.26 delay 1 second @20:51:57.28 AFV.select :holdingCoil @20:51:57.38 delay 1 second @20:51:58.41 AS.reconfigure ASfast @20:51:58.46 AS.seek 0ml @20:52:25.80 delay 3 seconds @20:52:28.83 AFV.select :carrierSolution @20:52:28.93 delay 2 seconds @20:52:30.96 AS.reconfigure ASconfig @20:52:31.02 AS.seek 1ml @20:53:28.84 delay 1 second @20:53:29.86 AFV.select :holdingCoil @20:53:29.96 delay 1 second @20:53:30.99 AS.reconfigure ASfast @20:53:31.04 AS.seek 0ml @20:53:58.36 delay 3 seconds @20:54:01.39 AFV.select :carrierSolution @20:54:01.49 delay 2 seconds @20:54:03.52 AS.reconfigure ASconfig @20:54:03.57 AS.seek 1ml @20:55:01.38 delay 1 second @20:55:02.40 AFV.select :holdingCoil @20:55:02.50 delay 1 second @20:55:03.53 AS.reconfigure ASfast @20:55:03.58 AS.seek 0ml @20:55:30.91 delay 3 seconds @20:55:33.94 AFV.select :carrierSolution @20:55:34.04 delay 2 seconds @20:55:36.07 AS.reconfigure ASconfig @20:55:36.13 AS.seek 1ml @20:56:33.89 delay 1 second @20:56:34.91 AFV.select :holdingCoil @20:56:35.01 delay 1 second @20:56:36.04 AS.reconfigure ASfast @20:56:36.09 AS.seek 0ml @20:57:03.42 delay 3 seconds @20:57:06.45 AFV.select :carrierSolution @20:57:06.55 delay 2 seconds @20:57:08.58 AS.reconfigure ASconfig @20:57:08.63 AS.seek 1ml @20:58:07.07 delay 1 second @20:58:08.09 AFV.select :holdingCoil @20:58:08.19 delay 1 second @20:58:09.22 AS.reconfigure ASnorm @20:58:09.27 AS.seek 0ml @20:59:00.01 Setting up PCR for IPc -- writing /var/log/esp/IPc20may1620h625ml.pcr @20:59:00.03 Building PCR Slug from ASV with MM3 mix, PP6 primer @20:59:00.04 Priming with carrier @20:59:00.05 ATV.dial ARV @20:59:00.65 ARV.dial :carrier @20:59:01.00 AS.seek 0.5ml @20:59:26.58 AFV.select :carrierSolution @20:59:26.69 delay 2 seconds @20:59:28.72 AS.reconfigure ASconfig @20:59:28.77 AS.seek 1ml @20:59:57.83 delay 1 second @20:59:58.85 AFV.select :holdingCoil @20:59:58.95 delay 1 second @20:59:59.98 ATV.dial :waste @21:00:01.55 AS.reconfigure ASnorm @21:00:01.61 AS.seek :empty @21:00:52.35 AFV.select :carrierSolution @21:00:52.46 delay 2 seconds @21:00:54.49 AS.reconfigure ASconfig @21:00:54.54 AS.seek 0.5ml @21:01:23.65 delay 1 second @21:01:24.67 AFV.select :holdingCoil @21:01:24.77 Building Slug @21:01:24.78 ATV.dial :ARV @21:01:26.33 delay 2 seconds @21:01:28.36 ARV.dial :air1 @21:01:28.75 delay 2 seconds @21:01:30.84 AS.reconfigure ASslow2 @21:01:30.90 AS.seek 0.506ml @21:01:37.16 delay 2 seconds @21:01:39.18 ARV.dial :MM3 @21:01:40.18 delay 2 seconds @21:01:42.21 Adding MM3 @21:01:42.29 AS.seek 0.524ml @21:02:00.77 delay 25 seconds @21:02:25.80 ARV.dial :PP6 @21:02:26.18 Adding PP6 @21:02:26.20 delay 2 seconds @21:02:28.28 AS.seek 0.53ml @21:02:34.63 delay 10 seconds @21:02:44.66 ARV.dial :air1 @21:02:45.43 adding ARV air @21:02:45.44 delay 2 seconds @21:02:47.53 AS.seek 0.536ml @21:02:53.69 delay 5 seconds @21:02:58.72 ARV.dial :carrier @21:02:59.10 delay 7 seconds @21:03:06.19 AS.seek 0.594ml @21:04:04.74 delay 5 seconds @21:04:09.77 ATV.dial :ASV @21:04:10.16 delay 2 seconds @21:04:12.25 AS.seek 0.6ml @21:04:18.60 delay 4 seconds @21:04:22.62 ATV.dial ARV @21:04:23.16 delay 2 seconds @21:04:25.19 ARV.dial :carrier @21:04:25.20 delay 2 seconds @21:04:27.29 AS.seek 0.65ml @21:05:18.02 delay 4 seconds @21:05:22.05 ATV.dial PCR @21:05:22.63 ASV.dial :waste1 @21:05:22.64 ARV.dial :air1 @21:05:23.03 Positioning Slug by 0.324ml @21:05:23.12 AS.seek 0.326ml @21:10:49.03 delay 5 seconds @21:10:54.06 ATV.dial ARV @21:10:54.81 ASV.dial :PCRlock @21:10:55.20 delay 5 seconds @21:11:00.23 Running IPc ProfileIPc thermocycling @21:11:00.24 PCR.program ProfileIPc @21:11:00.35 ProfileIPc should complete in < 1:36:15 @21:11:00.36 PCR.run 2:05:18.75 @22:29:13.29 PCR.off @22:29:13.84 PCR for IPc completed -- closed /var/log/esp/IPc20may1620h625ml.pcr @22:29:13.91 Cleaning PCR @22:29:13.92 AFV.select :carrierSolution @22:29:14.03 delay 2 seconds @22:29:16.06 AS.reconfigure ASconfig @22:29:16.11 AS.seek 0.5ml @22:29:26.43 delay 1 second @22:29:27.45 AFV.select :holdingCoil @22:29:27.55 ASV.dial :waste1 @22:29:27.93 ATV.dial :air3 @22:29:29.16 AS.reconfigure ASslow1 @22:29:29.21 AS.seek 0.51ml @22:29:31.54 delay 2 seconds @22:29:33.56 ATV.dial :bleach @22:29:34.16 AS.seek 0.81ml @22:30:34.79 delay 3 seconds @22:30:37.82 ATV.dial :air3 @22:30:38.36 delay 3 seconds @22:30:41.45 AS.seek 0.835ml @22:30:46.76 delay 3 seconds @22:30:49.79 ATV.dial :bleach @22:30:50.39 AS.seek 0.82ml @22:30:53.67 delay 3 seconds @22:30:56.70 ARV.dial :waste @22:30:57.28 delay 3 seconds @22:31:00.31 ATV.dial :ARV @22:31:01.15 AS.reconfigure ASconfig @22:31:01.20 AS.seek 0.47ml @22:31:21.61 AFV.select :carrierSolution @22:31:21.73 delay 2 seconds @22:31:23.77 AS.seek 1ml @22:31:54.51 delay 1 second @22:31:55.53 AFV.select :holdingCoil @22:31:55.63 delay 1 second @22:31:56.66 AS.reconfigure ASfast @22:31:56.71 AS.seek 0ml @22:32:24.04 AFV.select :carrierSolution @22:32:24.15 delay 2 seconds @22:32:26.18 AS.reconfigure ASconfig @22:32:26.23 AS.seek 1ml @22:33:24.03 delay 1 second @22:33:25.05 AFV.select :holdingCoil @22:33:25.15 delay 1 second @22:33:26.18 AS.reconfigure ASfast @22:33:26.23 AS.seek 0ml @22:33:53.58 AFV.select :carrierSolution @22:33:53.69 delay 2 seconds @22:33:55.72 AS.reconfigure ASconfig @22:33:56.40 AS.seek 1ml @22:34:54.19 delay 1 second @22:34:55.21 AFV.select :holdingCoil @22:34:55.31 delay 1 second @22:34:56.34 AS.reconfigure ASfast @22:34:56.39 AS.seek 0ml @22:35:23.73 ARV.dial between carrier and air1 @22:35:24.22 ATV.dial :air3 @22:35:25.46 AS.reconfigure ASslow1 @22:35:25.51 AS.seek 0.01ml @22:35:27.80 delay 2 seconds @22:35:29.82 ATV.dial :bleach @22:35:30.43 AS.seek 0.81ml @22:38:11.56 delay 3 seconds @22:38:14.59 ATV.dial :air3 @22:38:15.13 delay 3 seconds @22:38:18.22 AS.seek 0.835ml @22:38:23.52 delay 3 seconds @22:38:26.55 ATV.dial :bleach @22:38:27.15 AS.seek 0.82ml @22:38:30.45 delay 3 seconds @22:38:33.48 ATV.dial PCR @22:38:33.88 AS.reconfigure ASconfig @22:38:33.93 AS.seek :empty @22:39:21.39 PCR.program :bleachProfile @22:39:21.45 PCR program should complete in < 2:45 @22:39:21.46 PCR.run 10:00 @22:42:38.43 PCR.off @22:42:38.45 AFV.select :carrierSolution @22:42:38.56 delay 2 seconds @22:42:40.60 AS.seek 1ml @22:43:38.38 delay 1 second @22:43:39.40 AFV.select :holdingCoil @22:43:39.50 delay 1 second @22:43:40.53 AS.reconfigure ASfast @22:43:40.58 AS.seek 0ml @22:44:07.91 delay 3 seconds @22:44:10.94 AFV.select :carrierSolution @22:44:11.04 delay 2 seconds @22:44:13.07 AS.reconfigure ASconfig @22:44:13.13 AS.seek 1ml @22:45:10.91 delay 1 second @22:45:11.93 AFV.select :holdingCoil @22:45:12.03 delay 1 second @22:45:13.06 AS.reconfigure ASfast @22:45:13.11 AS.seek 0ml @22:45:40.43 delay 3 seconds @22:45:43.46 AFV.select :carrierSolution @22:45:43.56 delay 2 seconds @22:45:45.59 AS.reconfigure ASconfig @22:45:45.65 AS.seek 1ml @22:46:43.43 delay 1 second @22:46:44.45 AFV.select :holdingCoil @22:46:44.55 delay 1 second @22:46:45.58 AS.reconfigure ASfast @22:46:45.63 AS.seek 0ml @22:47:12.96 delay 3 seconds @22:47:15.99 AFV.select :carrierSolution @22:47:16.09 delay 2 seconds @22:47:18.12 AS.reconfigure ASconfig @22:47:18.17 AS.seek 1ml @22:48:15.93 delay 1 second @22:48:16.95 AFV.select :holdingCoil @22:48:17.05 delay 1 second @22:48:18.08 AS.reconfigure ASfast @22:48:18.13 AS.seek 0ml @22:48:45.48 delay 3 seconds @22:48:48.51 AFV.select :carrierSolution @22:48:48.61 delay 2 seconds @22:48:50.64 AS.reconfigure ASconfig @22:48:50.70 AS.seek 1ml @22:49:48.53 delay 1 second @22:49:49.55 AFV.select :holdingCoil @22:49:49.65 delay 1 second @22:49:50.68 AS.reconfigure ASnorm @22:49:50.73 AS.seek 0ml @22:50:41.48 Setting up PCR for IPc2 -- writing /var/log/esp/IPc220may1622h625ml.pcr @22:50:41.49 Building PCR Slug from ASV with MM2 mix, PP3 primer @22:50:41.51 Priming with carrier @22:50:41.52 ATV.dial ARV @22:50:42.10 ARV.dial :carrier @22:50:42.44 AS.seek 0.5ml @22:51:07.96 AFV.select :carrierSolution @22:51:08.07 delay 2 seconds @22:51:10.10 AS.reconfigure ASconfig @22:51:10.15 AS.seek 1ml @22:51:39.17 delay 1 second @22:51:40.19 AFV.select :holdingCoil @22:51:40.29 delay 1 second @22:51:41.32 ATV.dial :waste @22:51:42.89 AS.reconfigure ASnorm @22:51:42.94 AS.seek :empty @22:52:33.67 AFV.select :carrierSolution @22:52:33.78 delay 2 seconds @22:52:35.81 AS.reconfigure ASconfig @22:52:35.86 AS.seek 0.5ml @22:53:05.59 delay 1 second @22:53:06.62 AFV.select :holdingCoil @22:53:06.72 Building Slug @22:53:06.73 ATV.dial :ARV @22:53:08.29 delay 2 seconds @22:53:10.32 ARV.dial :air1 @22:53:10.71 delay 2 seconds @22:53:12.80 AS.reconfigure ASslow2 @22:53:12.86 AS.seek 0.506ml @22:53:19.12 delay 2 seconds @22:53:21.14 ARV.dial :MM2 @22:53:22.54 delay 2 seconds @22:53:24.57 Adding MM2 @22:53:24.64 AS.seek 0.524ml @22:53:43.06 delay 25 seconds @22:54:08.09 ARV.dial :PP3 @22:54:08.47 Adding PP3 @22:54:08.49 delay 2 seconds @22:54:10.57 AS.seek 0.53ml @22:54:16.76 delay 10 seconds @22:54:26.79 ARV.dial :air2 @22:54:27.17 adding ARV air @22:54:27.19 delay 2 seconds @22:54:29.27 AS.seek 0.536ml @22:54:35.47 delay 5 seconds @22:54:40.50 ARV.dial :carrier @22:54:41.70 delay 7 seconds @22:54:48.79 AS.seek 0.594ml @22:55:47.28 delay 5 seconds @22:55:52.31 ATV.dial :ASV @22:55:52.70 delay 2 seconds @22:55:54.79 AS.seek 0.6ml @22:56:01.12 delay 4 seconds @22:56:05.15 ATV.dial ARV @22:56:05.78 delay 2 seconds @22:56:07.81 ARV.dial :carrier @22:56:07.82 delay 2 seconds @22:56:09.91 AS.seek 0.65ml @22:57:00.70 delay 4 seconds @22:57:04.73 ATV.dial PCR @22:57:05.33 ASV.dial :waste1 @22:57:05.34 ARV.dial :air1 @22:57:05.75 Positioning Slug by 0.324ml @22:57:05.84 AS.seek 0.326ml @23:02:31.85 delay 5 seconds @23:02:36.88 ATV.dial ARV @23:02:37.63 ASV.dial :PCRlock @23:02:38.02 delay 5 seconds @23:02:43.05 Running IPc2 ProfileIPc thermocycling @23:02:43.06 PCR.program ProfileIPc @23:02:43.16 ProfileIPc should complete in < 1:36:15 @23:02:43.17 PCR.run 2:05:18.75 @00:20:43.33CEST17-May-20 PCR.off @00:20:43.90 PCR for IPc2 completed -- closed /var/log/esp/IPc220may1622h625ml.pcr @00:20:43.91 Full MFB Decontamination @00:20:43.92 Decon extraction column and decon AIV @00:20:43.94 SPE.hold 2:46:40,55C @00:20:43.99 ASV.dial :SPEdelivery @00:20:44.60 delay 20 seconds @00:21:04.63 AFV.select :carrierSolution @00:21:04.73 delay 2 seconds @00:21:06.76 AS.reconfigure ASconfig @00:21:06.81 AS.seek 0.5ml @00:21:17.13 delay 1 second @00:21:18.15 AFV.select :holdingCoil @00:21:18.25 ATV.dial :air1 @00:21:19.99 AS.reconfigure ASslow1 @00:21:20.04 AS.seek 0.52ml @00:21:24.33 ATV.dial :ASV @00:21:25.92 ASV.dial :SPErecovery @00:21:26.57 AS.seek 0.62ml @00:21:46.97 ATV.dial :waste @00:21:48.77 AS.reconfigure ASnorm @00:21:48.82 AS.seek :empty @00:22:20.39 AFV.select :holdingCoil @00:22:20.40 ATV.dial :waste @00:22:20.42 AS.reconfigure ASconfig @00:22:20.47 AS.seek 0ml @00:22:20.74 AFV.select :carrierSolution @00:22:20.86 delay 2 seconds @00:22:22.90 AS.seek 0.5ml @00:22:51.91 delay 1 second @00:22:52.93 AFV.select :holdingCoil @00:22:53.03 ATV.dial :air3 @00:22:53.70 AS.reconfigure ASslow1 @00:22:53.75 AS.seek 0.52ml @00:22:58.03 ATV.dial :air3 @00:22:58.11 AS.seek 0.53ml @00:23:00.39 delay 2 seconds @00:23:02.41 ATV.dial :bleach @00:23:02.87 AS.seek 0.71ml @00:23:39.33 delay 3 seconds @00:23:42.36 ATV.dial :air3 @00:23:42.89 delay 3 seconds @00:23:45.98 AS.seek 0.735ml @00:23:51.28 delay 3 seconds @00:23:54.31 ATV.dial :bleach @00:23:54.92 AS.seek 0.72ml @00:23:58.21 delay 3 seconds @00:24:01.24 ATV.dial ASV @00:24:02.22 ASV.dial :SPErecovery @00:24:02.30 AS.seek 0.365ml @00:25:13.94 ATV.dial :waste @00:25:15.54 AS.reconfigure ASnorm @00:25:15.59 AS.seek :empty @00:25:34.35 ATV.dial :air3 @00:25:35.01 AS.reconfigure ASslow1 @00:25:35.06 AS.seek 0.009ml @00:25:37.38 delay 2 seconds @00:25:39.41 ATV.dial :bleach @00:25:39.86 AS.seek 0.579ml @00:27:34.73 delay 3 seconds @00:27:37.76 ATV.dial :air3 @00:27:38.45 delay 3 seconds @00:27:41.53 AS.seek 0.604ml @00:27:46.83 delay 3 seconds @00:27:49.86 ATV.dial :bleach @00:27:50.47 AS.seek 0.589ml @00:27:53.79 delay 3 seconds @00:27:56.82 ATV.dial :ASV @00:27:57.80 ASV.dial :wasteR @00:27:58.19 AS.seek 0ml @00:29:56.95 delay 1:00 @00:30:56.98 ATV.dial ASV @00:30:56.99 AFV.select :carrierSolution @00:30:57.09 delay 2 seconds @00:30:59.12 AS.reconfigure ASconfig @00:30:59.17 AS.seek 1ml @00:31:56.92 delay 1 second @00:31:57.94 AFV.select :holdingCoil @00:31:58.04 delay 1 second @00:31:59.07 ASV.dial :wasteR @00:31:59.09 AS.reconfigure ASslow1 @00:31:59.13 AS.seek 0ml @00:35:20.53 delay 10 seconds @00:35:30.56 ATV.dial :air3 @00:35:32.00 AS.seek 0.01ml @00:35:34.29 delay 2 seconds @00:35:36.31 ATV.dial :bleach @00:35:36.93 AS.seek 0.58ml @00:37:31.83 delay 3 seconds @00:37:34.86 ATV.dial :air3 @00:37:35.40 delay 3 seconds @00:37:38.49 AS.seek 0.605ml @00:37:43.80 delay 3 seconds @00:37:46.83 ATV.dial :bleach @00:37:47.44 AS.seek 0.59ml @00:37:50.76 delay 3 seconds @00:37:53.79 ATV.dial ASV @00:37:54.78 ASV.dial :SPEdelivery @00:37:55.16 AS.seek 0ml @00:39:54.06 delay 1:00 @00:40:54.09 AFV.select :carrierSolution @00:40:54.19 delay 2 seconds @00:40:56.22 AS.reconfigure ASconfig @00:40:56.27 AS.seek 1ml @00:41:54.08 delay 1 second @00:41:55.10 AFV.select :holdingCoil @00:41:55.20 delay 1 second @00:41:56.23 ATV.dial ASV @00:41:56.24 ASV.dial :SPEdelivery @00:41:56.26 AS.reconfigure ASslow1 @00:41:56.30 AS.seek 0ml @00:45:17.66 delay 10 seconds @00:45:27.69 ATV.dial :air2 @00:45:28.48 AS.reconfigure ASslow0 @00:45:28.53 AS.seek 0.8ml @00:46:49.30 delay 3 seconds @00:46:52.33 ATV.dial :ASV @00:46:53.27 ASV.dial :SPEdelivery @00:46:53.29 AS.reconfigure ASslow1 @00:46:53.34 AS.seek 0.03ml @00:49:28.55 delay 20 seconds @00:49:48.58 ATV.dial :waste,via: :air3 @00:49:50.19 AS.reconfigure ASconfig @00:49:50.25 AS.seek :empty @00:49:52.31 SPE.coast @00:49:52.40 Removing Bleach from Mixing Coil @00:49:52.41 AFV.select :carrierSolution @00:49:52.52 delay 2 seconds @00:49:54.56 AS.seek 0.5ml @00:50:23.66 delay 1 second @00:50:24.68 AFV.select :holdingCoil @00:50:24.78 ATV.dial :ASV @00:50:26.52 ASV.dial :SPErecovery @00:50:27.15 AS.reconfigure ASslow1 @00:50:27.20 AS.seek 0.73ml @00:51:13.76 ASV.dial :wasteD @00:51:14.41 AS.reconfigure ASnorm @00:51:14.47 AS.seek 0ml @00:51:51.59 AFV.select :carrierSolution @00:51:51.70 delay 2 seconds @00:51:53.73 AS.reconfigure ASconfig @00:51:53.78 AS.seek 0.5ml @00:52:22.82 delay 1 second @00:52:23.84 AFV.select :holdingCoil @00:52:23.94 ATV.dial ASV @00:52:23.95 ASV.dial :SPErecovery @00:52:24.59 AS.reconfigure ASslow0 @00:52:24.64 AS.seek 0.3ml @00:52:45.10 ATV.dial :air2 @00:52:45.95 AS.reconfigure ASslow1 @00:52:46.00 AS.seek 0.32ml @00:52:50.29 ATV.dial ASV @00:52:51.30 AS.reconfigure ASslow0 @00:52:51.35 AS.seek 0.57ml @00:53:17.43 ASV.dial :wasteD @00:53:18.02 AS.reconfigure ASnorm @00:53:18.07 AS.seek 0ml @00:53:47.16 AFV.select :carrierSolution @00:53:47.27 delay 2 seconds @00:53:49.30 AS.reconfigure ASconfig @00:53:49.35 AS.seek 0.5ml @00:54:18.40 delay 1 second @00:54:19.42 AFV.select :holdingCoil @00:54:19.52 ATV.dial ASV @00:54:19.53 ASV.dial :SPErecovery @00:54:20.17 AS.reconfigure ASslow0 @00:54:20.22 AS.seek 0.3ml @00:54:40.67 ATV.dial :air2 @00:54:41.67 AS.reconfigure ASslow1 @00:54:41.72 AS.seek 0.32ml @00:54:45.96 ATV.dial ASV @00:54:46.97 AS.reconfigure ASslow0 @00:54:47.03 AS.seek 0.57ml @00:55:12.51 ASV.dial :wasteD @00:55:13.10 AS.reconfigure ASnorm @00:55:13.15 AS.seek 0ml @00:55:42.21 ATV.dial :air2 @00:55:43.13 AS.reconfigure ASslow0 @00:55:43.19 AS.seek 0.3ml @00:56:13.68 ATV.dial :ASV @00:56:14.60 ASV.dial :wasteD @00:56:14.62 AS.reconfigure ASconfig @00:56:14.67 AS.seek 0.05ml @00:56:29.35 ATV.dial :waste @00:56:31.11 AS.seek 0ml @00:56:34.28 ASV.dial :SPEdelivery @00:56:35.24 deconLysateINT @00:56:35.25 ATV.dial :air3 @00:56:35.92 AS.reconfigure ASslow1 @00:56:35.97 AS.seek 0.01ml @00:56:38.29 delay 2 seconds @00:56:40.31 ATV.dial :bleach @00:56:40.77 AS.seek 0.96ml @00:59:52.06 delay 3 seconds @00:59:55.09 ATV.dial :air3 @00:59:55.62 delay 3 seconds @00:59:58.71 AS.seek 0.985ml @01:00:04.01 delay 3 seconds @01:00:07.04 ATV.dial :bleach @01:00:07.65 AS.seek 0.97ml @01:00:10.95 delay 3 seconds @01:00:13.98 ATV.dial AIV @01:00:15.17 AIV.dial :coilToMFB @01:00:15.18 AWV.select :waste @01:00:15.20 AFV.select :carrierSolution @01:00:15.31 delay 2 seconds @01:00:17.34 AS.reconfigure ASconfig @01:00:17.39 AS.seek 1ml @01:00:19.47 delay 1 second @01:00:20.49 AFV.select :holdingCoil @01:00:20.59 delay 1 second @01:00:21.62 AS.reconfigure ASslow0 @01:00:21.67 AS.seek :empty @01:02:02.65 AFV.select :carrierSolution @01:02:02.76 delay 2 seconds @01:02:04.79 AS.reconfigure ASconfig @01:02:04.84 AS.seek 1ml @01:03:02.64 delay 1 second @01:03:03.66 AFV.select :holdingCoil @01:03:03.76 delay 1 second @01:03:04.79 AS.reconfigure ASslow0 @01:03:04.84 AS.seek :empty @01:04:45.81 AFV.select :carrierSolution @01:04:45.92 delay 2 seconds @01:04:47.95 AS.reconfigure ASconfig @01:04:48.00 AS.seek 1ml @01:05:45.83 delay 1 second @01:05:46.85 AFV.select :holdingCoil @01:05:46.95 delay 1 second @01:05:47.98 AS.reconfigure ASslow0 @01:05:48.03 AS.seek :empty @01:07:29.03 ATV.dial :air2 @01:07:29.68 AS.reconfigure ASslow1 @01:07:29.73 AS.seek 0.009ml @01:07:32.02 AS.reconfigure ASslow0 @01:07:32.07 AS.seek 1ml @01:09:12.00 ATV.dial AIV @01:09:12.75 AS.seek 0.02ml @01:10:51.61 ATV.dial :waste @01:10:53.19 AS.seek 0ml @01:10:55.50 AWV.select :air @01:10:55.67 Cleaning PCR @01:10:55.68 AFV.select :carrierSolution @01:10:55.78 delay 2 seconds @01:10:58.40 AS.reconfigure ASconfig @01:10:58.47 AS.seek 0.5ml @01:11:27.50 delay 1 second @01:11:28.52 AFV.select :holdingCoil @01:11:28.62 ASV.dial :waste1 @01:11:29.00 ATV.dial :air3 @01:11:29.67 AS.reconfigure ASslow1 @01:11:29.72 AS.seek 0.51ml @01:11:32.07 delay 2 seconds @01:11:34.09 ATV.dial :bleach @01:11:34.54 AS.seek 0.81ml @01:12:35.13 delay 3 seconds @01:12:38.16 ATV.dial :air3 @01:12:38.69 delay 3 seconds @01:12:41.78 AS.seek 0.835ml @01:12:47.08 delay 3 seconds @01:12:50.10 ATV.dial :bleach @01:12:50.70 AS.seek 0.82ml @01:12:53.97 delay 3 seconds @01:12:57.00 ARV.dial :waste @01:12:57.58 delay 3 seconds @01:13:00.61 ATV.dial :ARV @01:13:01.47 AS.reconfigure ASconfig @01:13:01.52 AS.seek 0.47ml @01:13:21.94 AFV.select :carrierSolution @01:13:22.05 delay 2 seconds @01:13:24.09 AS.seek 1ml @01:13:54.82 delay 1 second @01:13:55.84 AFV.select :holdingCoil @01:13:55.94 delay 1 second @01:13:56.97 AS.reconfigure ASfast @01:13:57.02 AS.seek 0ml @01:14:24.35 AFV.select :carrierSolution @01:14:24.46 delay 2 seconds @01:14:26.49 AS.reconfigure ASconfig @01:14:26.54 AS.seek 1ml @01:15:24.29 delay 1 second @01:15:25.31 AFV.select :holdingCoil @01:15:25.41 delay 1 second @01:15:26.44 AS.reconfigure ASfast @01:15:26.49 AS.seek 0ml @01:15:53.84 AFV.select :carrierSolution @01:15:53.95 delay 2 seconds @01:15:55.98 AS.reconfigure ASconfig @01:15:56.03 AS.seek 1ml @01:16:53.87 delay 1 second @01:16:54.89 AFV.select :holdingCoil @01:16:54.99 delay 1 second @01:16:56.02 AS.reconfigure ASfast @01:16:56.07 AS.seek 0ml @01:17:23.41 ARV.dial between carrier and air1 @01:17:23.88 ATV.dial :air3 @01:17:25.13 AS.reconfigure ASslow1 @01:17:25.18 AS.seek 0.01ml @01:17:27.49 delay 2 seconds @01:17:29.51 ATV.dial :bleach @01:17:30.11 AS.seek 0.81ml @01:20:11.26 delay 3 seconds @01:20:14.29 ATV.dial :air3 @01:20:14.82 delay 3 seconds @01:20:17.91 AS.seek 0.835ml @01:20:23.19 delay 3 seconds @01:20:26.22 ATV.dial :bleach @01:20:26.83 AS.seek 0.82ml @01:20:30.17 delay 3 seconds @01:20:33.20 ATV.dial PCR @01:20:33.59 AS.reconfigure ASconfig @01:20:33.64 AS.seek :empty @01:21:21.21 PCR.program :bleachProfile @01:21:21.27 PCR program should complete in < 2:45 @01:21:21.28 PCR.run 10:00 @01:24:50.37 PCR.off @01:24:50.41 AFV.select :carrierSolution @01:24:50.52 delay 2 seconds @01:24:52.56 AS.seek 1ml @01:25:50.41 delay 1 second @01:25:51.43 AFV.select :holdingCoil @01:25:51.53 delay 1 second @01:25:52.56 AS.reconfigure ASfast @01:25:52.61 AS.seek 0ml @01:26:19.95 delay 3 seconds @01:26:22.98 AFV.select :carrierSolution @01:26:23.08 delay 2 seconds @01:26:25.64 AS.reconfigure ASconfig @01:26:25.77 AS.seek 1ml @01:27:23.61 delay 1 second @01:27:24.63 AFV.select :holdingCoil @01:27:24.73 delay 1 second @01:27:25.76 AS.reconfigure ASfast @01:27:25.81 AS.seek 0ml @01:27:53.16 delay 3 seconds @01:27:56.19 AFV.select :carrierSolution @01:27:56.29 delay 2 seconds @01:27:58.32 AS.reconfigure ASconfig @01:27:58.37 AS.seek 1ml @01:28:56.16 delay 1 second @01:28:57.18 AFV.select :holdingCoil @01:28:57.28 delay 1 second @01:28:58.31 AS.reconfigure ASfast @01:28:58.36 AS.seek 0ml @01:29:25.69 delay 3 seconds @01:29:28.72 AFV.select :carrierSolution @01:29:28.82 delay 2 seconds @01:29:30.85 AS.reconfigure ASconfig @01:29:30.91 AS.seek 1ml @01:30:28.72 delay 1 second @01:30:29.74 AFV.select :holdingCoil @01:30:29.84 delay 1 second @01:30:30.87 AS.reconfigure ASfast @01:30:30.92 AS.seek 0ml @01:30:58.25 delay 3 seconds @01:31:01.28 AFV.select :carrierSolution @01:31:01.38 delay 2 seconds @01:31:03.41 AS.reconfigure ASconfig @01:31:03.46 AS.seek 1ml @01:32:01.25 delay 1 second @01:32:02.27 AFV.select :holdingCoil @01:32:02.37 delay 1 second @01:32:03.40 AS.reconfigure ASnorm @01:32:03.45 AS.seek 0ml @01:32:54.17 AFV.select :carrierSolution @01:32:54.28 delay 2 seconds @01:32:56.32 AS.reconfigure ASconfig @01:32:56.36 AS.seek 1ml @01:33:54.19 delay 1 second @01:33:55.21 AFV.select :holdingCoil @01:33:55.31 delay 1 second @01:33:56.34 ATV.dial :waste @01:33:57.51 AS.reconfigure ASnorm @01:33:57.55 AS.seek 0.5ml @01:34:23.10 ATV.dial :waste2 @01:34:24.33 AS.seek 0ml @01:34:49.82 AS.coast @01:34:49.86 SS.coast @01:34:49.90 CC.coast @01:34:49.94 CS.coast @01:34:49.98 PC.coast @01:34:50.02 PS.coast @01:34:50.06 Forearm.coast @01:34:50.10 Elbow.coast @01:34:50.50 Gate.power :core,:OFF @01:34:50.52 Gate.power :raw,:OFF @01:34:50.53 Shutdown @01:34:50.54 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @03:37:52.75 Session Begun @03:38:27.08 -> GoodNews.email "trigger data upload" @03:38:27.09 GoodNews.email "trigger data upload" @03:41:18.25 -> quit @03:41:18.27 Session Ended @10:00:47.72
Powering core bus @10:00:52.75 Gate.power :raw,:ON @10:00:53.42 SPE.coast @10:00:53.49 SPE.configure SPEconfig @10:00:53.59 AS.configure ASconfig @10:00:53.69 SS.configure SSconfig @10:00:53.79 CC.configure CCconfig @10:00:53.89 CS.configure CSconfig @10:00:53.99 PC.configure PCconfig @10:00:54.10 PS.configure PSconfig @10:00:54.22 Forearm.configure LooseForearm @10:00:54.31 Elbow.configure LooseElbow @10:00:54.44 SC.configure SCloose @10:00:54.53 SE.configure SEconfig @10:00:54.58 CH.coast @10:00:54.64 CH.configure CHconfig @10:00:54.67 PH.coast @10:00:54.73 PH.configure PHconfig @10:00:54.77 PCR.off @10:00:54.80 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:54.84 PCR.caltemp= 2.8814,0.0372,0 @10:00:54.87 All dwarf controllers running firmware version 3.75 @10:00:54.98 Valve.reset @10:00:55.15 PCR.off @10:00:55.18 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:55.21 PCR.caltemp= 2.8814,0.0372,0 @10:00:55.45 PSV.dial :air @10:00:55.48 Intake.select :close @10:00:55.56 Exhaust.select :close @10:00:55.59 CTV.dial :air @10:00:55.67
Forearm.reconfigure LooseForearm @10:00:55.73 Forearm.home.seek :retract @10:00:55.76 CSV.dial :waste1 @10:00:55.88 SSV.dial :spit,avoiding: :exhaust @10:00:56.00 PS.reconfigure PSconfig @10:00:56.06 PS.home.seek 0ml @10:00:56.53 Hand.close @10:00:56.56
Elbow.jog 1500 @10:00:56.73 CS.reconfigure CSfast @10:00:56.77 SS.reconfigure SSconfig @10:00:56.86 CS.home.seek 0ml @10:00:56.91 SS.home.seek 0ml @10:00:57.31
Elbow.reconfigure LooseElbow @10:00:57.36 Elbow.home.seek :clear @10:01:00.77 SE.reconfigure SEconfig @10:01:00.82 SE.home.seek :down @10:01:01.41 SC.reconfigure SCsloppy @10:01:01.46 SC.home.seek :initial @10:01:03.82 Forearm.seek :retract @10:01:03.89 Elbow.seek :hammer @10:01:05.08 PS.coast @10:01:05.22
Forearm.reconfigure TightForearm @10:01:05.26 Forearm.seek 5 + 350 counts @10:01:06.36 Forearm.seek :retract @10:01:07.57 PC.reconfigure PCconfig @10:01:07.58
CC.reconfigure CCconfig @10:01:07.68 CC.home.seek :open @10:01:07.72 PC.home.seek :open @10:01:11.23
Phase [stdphase] @10:01:11.26 GoodNews.email "At 10:01:11 began in tube 2: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:124\n{1 => 12, 2 => 10}\nCan@10:01:11, 7.5C, 42% humidity, 14.3psia, 14.001V, 0.676A, 0.676A avg, 9.47W",Subject: "Started 20invasivedeployfinal phase stdphase" @10:01:11.28CEST17-May-20
===> STARTED Superphase (phase #4) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @10:01:11.32 Starting initialPurge @10:01:11.33 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:01:11.41 Hand.open @10:01:12.16 Elbow.reconfigure TightElbow @10:01:12.22 Elbow.seek FlushPuck::Garage @10:01:14.32 Elbow.reconfigure LooseElbow @10:01:14.55 Forearm.reconfigure LooseForearm @10:01:14.63 Forearm.seek :garage @10:01:15.64 Hand.close @10:01:16.45 Forearm.seek :retract @10:01:17.48 Elbow.reconfigure TightElbow @10:01:17.53 Elbow.seek CC @10:01:20.62 Elbow.reconfigure LooseElbow @10:01:20.84 Forearm.reconfigure TightForearm @10:01:20.92 Forearm.seek CC @10:01:22.61 CC.seek :closed @10:01:27.04 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.92 @10:01:27.25 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[46:27->20] @10:01:27.31 Hand.open @10:01:28.16 Forearm.reconfigure LooseForearm @10:01:28.25 Forearm.seek :retract @10:01:29.28 Flushing the Collection Valve::Manifold @10:01:29.57 CSV.dial CSR @10:01:31.16 Pulling 5ml of CSR.flush @10:01:31.19 CSR.select :flush @10:01:31.39 CS.reconfigure CSconfig @10:01:31.49 CS.seek 5ml @10:01:45.35 delay 5 seconds @10:01:50.38 CSR.select :air @10:01:50.69 CS.reconfigure CSfast @10:01:50.79 CS.seek 7.501ml @10:01:57.44 CSV.dial :waste @10:01:58.85 CS.reconfigure CSconfig @10:01:58.95 CS.seek 0ml @10:02:19.14 Purging external Intake @10:02:19.27 CSV.dial :flush @10:02:19.74 CS.reconfigure CSfast @10:02:19.79 CS.seek 10ml @10:02:42.70 delay 5 seconds @10:02:47.73 CSV.dial CBV @10:02:47.77
CBV.dial CSV @10:02:48.54 CTV.dial :intake @10:02:49.31 Intake.select :open @10:02:49.43 CS.seek 0ml @10:03:13.73 Intake.select :close @10:03:13.84 CTV.dial :air @10:03:14.61 Flushing upper manifold @10:03:14.74 CSV.dial :flush @10:03:16.02 CS.seek 5ml @10:03:28.14 delay 5 seconds @10:03:33.17 CSV.dial :air @10:03:33.71 CS.seek 7ml @10:03:39.23 CSV.dial CBV @10:03:39.27
CBV.dial CSV @10:03:40.31 CTV.dial :air @10:03:40.33 CS.seek 0ml @10:03:57.10 delay 7 seconds @10:04:04.14 CS.seek 3ml @10:04:11.79 CSV.dial :waste @10:04:12.26 CS.seek 0ml @10:04:19.95 CSV.dial :flush @10:04:20.41 CS.seek 3ml @10:04:28.08 delay 5 seconds @10:04:33.11 CSV.dial :air @10:04:33.76 CS.seek 5.001ml @10:04:39.23 CSV.dial :air @10:04:39.25 CS.seek 0ml @10:04:51.23 delay 7 seconds @10:04:58.27 CS.seek 3ml @10:05:05.95 CSV.dial :waste @10:05:06.70 CS.seek 0ml @10:05:14.33 Garaging FlushPuck in Collection Clamp @10:05:14.41 Elbow.reconfigure TightElbow @10:05:14.46 Elbow.seek CC @10:05:14.72 Elbow.reconfigure LooseElbow @10:05:14.83 Forearm.seek CC @10:05:15.82 Hand.close @10:05:16.38 CC.seek :open @10:05:20.74 Forearm.seek :retract @10:05:21.77 Elbow.reconfigure TightElbow @10:05:21.82 Elbow.seek :garage @10:05:25.03 Elbow.reconfigure LooseElbow @10:05:25.13 Forearm.reconfigure TightForearm @10:05:25.18 Forearm.seek :garage @10:05:26.32 Hand.open @10:05:27.07 Forearm.reconfigure LooseForearm @10:05:27.11 Forearm.seek :retract @10:05:28.22 Starting mfb @10:05:28.24 pcrBAC sampling at most 1500ml, wcr at most 1500ml @10:05:28.27 BACfans.email "Started mfb in tube 2 at 10:05:28CEST17-May-20\n{1 => 12, 2 => 10}\nCan@10:04:26, 7.5C, 43% humidity, 14.3psia, 13.982V, 0.720A, 0.720A avg, 10.07W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @10:05:28.36
Loading sh1 puck from tube 2 into Collection Clamp @10:05:28.48 Readying the MFB @10:05:28.54 Homing MFB Syringe @10:05:28.55 AFV.select :holdingCoil @10:05:28.84 ATV.dial :air2 @10:05:29.08
SC.reconfigure SCloose @10:05:29.32 Elbow.reconfigure TightElbow @10:05:29.40 Elbow.seek 2 @10:05:29.54 AS.reconfigure ASconfig @10:05:29.59 AS.home.seek :home @10:05:31.50
Forearm.reconfigure TightForearm @10:05:31.56 Forearm.seek 2 + 700 counts @10:05:33.43 SE.reconfigure SEpick @10:05:33.51 SE.seek beamBreak @10:05:35.11 AFV.select :carrierSolution @10:05:35.26 delay 2 seconds @10:05:35.78
SE.configure SEconfig @10:05:36.25 Elbow.seek 2 @10:05:36.70 Forearm.seek 2 + 300 counts @10:05:36.99 Forearm.reconfigure LooseForearm @10:05:37.12 Hand.close @10:05:37.33 AS.seek 1ml @10:05:37.81 SE.seek :down @10:05:38.32
Forearm.seek :retract @10:05:39.32 Elbow.seek CC @10:05:39.89 SE.coast @10:05:41.10
Elbow.reconfigure LooseElbow @10:05:41.35 Forearm.reconfigure TightForearm @10:05:41.50 Forearm.seek CC @10:05:43.27 CC.seek :closed @10:05:47.63 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.06 @10:05:47.65 Hand.open @10:05:48.57 Forearm.reconfigure LooseForearm @10:05:48.66 Forearm.seek :retract @10:05:49.78 SC.coast @10:05:49.86 collectSample 1500ml w/bubble point of 55psi @10:05:49.89 Priming 7ml @10:05:49.90 Intake.select :close @10:05:49.93 Exhaust.select :close @10:05:49.96 SS.hold @10:05:50.45 Calibrating pressure and temperature sensors @10:05:50.49 CTV.dial :air @10:05:50.56 SSV.dial :spit,avoiding: :exhaust @10:05:50.60 SS.reconfigure SSfast @10:05:50.70 SS.seek 5ml @10:05:53.32 SSV.dial CBV @10:05:53.41
CBV.dial SSV @10:05:54.89 delay 3.8 seconds @10:05:58.99 SSV.dial :spit @10:06:00.42 SS.seek 0ml @10:06:03.05 SSV.dial CBV @10:06:04.44 CTV.dial :intake @10:06:05.29 SSV.dial CBV @10:06:05.34
CBV.dial SSV @10:06:05.37 Allowing 40s for 7.0ml of remaining 7.0ml @10:06:05.40 Intake.select :open @10:06:07.70 Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:06:07.76 SS.reconfigure SSinhale.with maxSpeed: 150 @10:06:07.86 SS.seek 7ml @10:06:11.38 Vacuum too high -- waiting up to 40s @10:06:14.01 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:06:14.07 SS.reconfigure SSinhale.with maxSpeed: 135 @10:06:14.16 SS.seek 7ml @10:06:17.36 Vacuum too high -- waiting up to 40s @10:06:19.97 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:06:20.02 SS.reconfigure SSinhale.with maxSpeed: 122 @10:06:20.12 SS.seek 7ml @10:06:20.94 Stroke completed -- waiting for pressure to equalize ... @10:06:23.40 Intake.select :close @10:06:23.67 SSV.dial :exhaust @10:06:24.51 Exhaust.select :open @10:06:24.68 SS.reconfigure SSexhale @10:06:24.77 SS.seek 0ml @10:06:28.62 Exhaust.select :close @10:06:28.78 Sampling 1500.0ml @10:06:28.84 Intake.select :close @10:06:28.87 Exhaust.select :close @10:06:28.89 SS.reconfigure SSconfig @10:06:28.99 SS.hold @10:06:29.45 CTV.dial :intake @10:06:29.48 SSV.dial CBV @10:06:29.54
CBV.dial SSV @10:06:30.30 delay 1 second @10:06:30.41
Allowing 130s for 25.0ml of remaining 1500.0ml @10:06:30.44 Intake.select :open @10:06:31.33 AFV.select :holdingCoil @10:06:31.46 delay 1 second @10:06:32.49 ATV.dial :waste @10:06:32.75
Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:06:32.81 SS.reconfigure SSinhale.with maxSpeed: 122 @10:06:32.91 SS.seek 25ml @10:06:33.58 AS.reconfigure ASnorm @10:06:33.72 AS.home.seek :empty @10:06:36.48
Vacuum too high -- waiting up to 40s @10:06:38.34 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:06:38.36 SS.reconfigure SSinhale.with maxSpeed: 110 @10:06:38.41 SS.seek 25ml @10:06:40.91 Vacuum too high -- waiting up to 40s @10:06:43.02 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:06:43.03 SS.reconfigure SSinhale.with maxSpeed: 99 @10:06:43.08 SS.seek 25ml @10:06:45.73 Vacuum too high -- waiting up to 40s @10:06:47.80 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:06:48.37 SS.reconfigure SSinhale.with maxSpeed: 89 @10:06:48.43 SS.seek 25ml @10:06:51.78 Vacuum too high -- waiting up to 40s @10:06:53.66 SS.reconfigure SSinhale.with maxSpeed: 80 @10:06:53.71 SS.seek 25ml @10:06:56.80 Vacuum too high -- waiting up to 40s @10:06:58.66 SS.reconfigure SSinhale.with maxSpeed: 72 @10:06:58.71 SS.seek 25ml @10:07:02.23 Vacuum too high -- waiting up to 40s @10:07:04.06 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:07:04.07 SS.reconfigure SSinhale.with maxSpeed: 65 @10:07:04.12 SS.seek 25ml @10:07:07.32 Vacuum too high -- waiting up to 40s @10:07:09.15 SS.reconfigure SSinhale.with maxSpeed: 59 @10:07:09.20 SS.seek 25ml @10:07:12.78 Vacuum too high -- waiting up to 40s @10:07:14.36 SS.reconfigure SSinhale.with maxSpeed: 53 @10:07:14.41 SS.seek 25ml @10:07:18.22 Stroke completed -- waiting for pressure to equalize ... @10:07:22.69 Intake.select :close @10:07:22.85 SSV.dial :exhaust @10:07:23.66 Exhaust.select :open @10:07:23.78 SS.reconfigure SSexhale @10:07:23.82 SS.seek 0ml @10:07:26.87 ATV.dial :waste @10:07:26.89 ARV.dial between carrier and air1 @10:07:26.98 ASV.dial :waste1 @10:07:32.74
Exhaust.select :close @10:07:32.87 SSV.dial CBV @10:07:32.89
CBV.dial SSV @10:07:33.69 Allowing 130s for 25.0ml of remaining 1475.0ml @10:07:33.70 Intake.select :open @10:07:35.87 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:07:35.89 SS.reconfigure SSinhale.with maxSpeed: 53 @10:07:35.94 SS.seek 25ml @10:07:41.82 Vacuum too high -- waiting up to 40s @10:07:43.41 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:07:43.42 SS.reconfigure SSinhale.with maxSpeed: 48 @10:07:43.48 SS.seek 25ml @10:07:48.79 Vacuum too high -- waiting up to 40s @10:07:50.63 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:07:50.65 SS.reconfigure SSinhale.with maxSpeed: 43 @10:07:50.70 SS.seek 25ml @10:07:57.31 Vacuum too high -- waiting up to 40s @10:07:59.14 SS.reconfigure SSinhale.with maxSpeed: 39 @10:07:59.19 SS.seek 25ml @10:08:06.73 Vacuum too high -- waiting up to 40s @10:08:08.55 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:08:08.57 SS.reconfigure SSinhale.with maxSpeed: 35 @10:08:08.61 SS.seek 25ml @10:08:18.38 Vacuum too high -- waiting up to 40s @10:08:20.27 SS.reconfigure SSinhale.with maxSpeed: 32 @10:08:20.32 SS.seek 25ml @10:08:32.63 Stroke completed -- waiting for pressure to equalize ... @10:08:38.89 Intake.select :close @10:08:39.05 SSV.dial :exhaust @10:08:39.86 Exhaust.select :open @10:08:39.98 SS.reconfigure SSexhale @10:08:40.02 SS.seek 0ml @10:08:48.82 Exhaust.select :close @10:08:48.96 SSV.dial CBV @10:08:48.98
CBV.dial SSV @10:08:49.80 Allowing 130s for 25.0ml of remaining 1450.0ml @10:08:49.81 Intake.select :open @10:08:51.98 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:08:52.00 SS.reconfigure SSinhale.with maxSpeed: 32 @10:08:52.05 SS.seek 25ml @10:09:07.51 Vacuum too high -- waiting up to 40s @10:09:09.34 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:09:09.36 SS.reconfigure SSinhale.with maxSpeed: 29 @10:09:09.40 SS.seek 25ml @10:09:39.22 Vacuum too high -- waiting up to 40s @10:09:41.30 SS.reconfigure SSinhale.with maxSpeed: 26 @10:09:41.35 SS.seek 25ml @10:09:57.71 Stroke completed -- waiting for pressure to equalize ... @10:10:03.41 Intake.select :close @10:10:03.56 SSV.dial :exhaust @10:10:04.36 Exhaust.select :open @10:10:04.48 SS.reconfigure SSexhale @10:10:04.52 SS.seek 0ml @10:10:13.46 Exhaust.select :close @10:10:13.58 SSV.dial CBV @10:10:13.60
CBV.dial SSV @10:10:14.43 Allowing 130s for 25.0ml of remaining 1425.0ml @10:10:14.44 Intake.select :open @10:10:16.62 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:10:16.63 SS.reconfigure SSinhale.with maxSpeed: 26 @10:10:16.68 SS.seek 25ml @10:11:23.87 Stroke completed -- waiting for pressure to equalize ... @10:11:29.48 Intake.select :close @10:11:29.64 SSV.dial :exhaust @10:11:30.44 Exhaust.select :open @10:11:30.56 SS.reconfigure SSexhale @10:11:30.60 SS.seek 0ml @10:11:39.64 Exhaust.select :close @10:11:39.76 SSV.dial CBV @10:11:39.79
CBV.dial SSV @10:11:40.58 Allowing 130s for 25.0ml of remaining 1400.0ml @10:11:40.59 Intake.select :open @10:11:42.77 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:11:42.78 SS.reconfigure SSinhale.with maxSpeed: 26 @10:11:42.83 SS.seek 25ml @10:12:50.08 Stroke completed -- waiting for pressure to equalize ... @10:12:55.75 Intake.select :close @10:12:55.91 SSV.dial :exhaust @10:12:56.71 Exhaust.select :open @10:12:56.83 SS.reconfigure SSexhale @10:12:56.88 SS.seek 0ml @10:13:05.63 Exhaust.select :close @10:13:05.75 SSV.dial CBV @10:13:05.80
CBV.dial SSV @10:13:06.57 Allowing 130s for 25.0ml of remaining 1375.0ml @10:13:06.58 Intake.select :open @10:13:08.75 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:13:08.77 SS.reconfigure SSinhale.with maxSpeed: 26 @10:13:08.82 SS.seek 25ml @10:14:16.43 Stroke completed -- waiting for pressure to equalize ... @10:14:22.12 Intake.select :close @10:14:22.27 SSV.dial :exhaust @10:14:23.07 Exhaust.select :open @10:14:23.19 SS.reconfigure SSexhale @10:14:23.23 SS.seek 0ml @10:14:32.16 Exhaust.select :close @10:14:32.28 SSV.dial CBV @10:14:32.30
CBV.dial SSV @10:14:33.11 Allowing 130s for 25.0ml of remaining 1350.0ml @10:14:33.12 Intake.select :open @10:14:35.30 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:14:35.31 SS.reconfigure SSinhale.with maxSpeed: 26 @10:14:35.36 SS.seek 25ml @10:15:43.12 Stroke completed -- waiting for pressure to equalize ... @10:15:48.17 Intake.select :close @10:15:48.33 SSV.dial :exhaust @10:15:49.13 Exhaust.select :open @10:15:49.25 SS.reconfigure SSexhale @10:15:49.30 SS.seek 0ml @10:15:58.88 Exhaust.select :close @10:15:59.00 SSV.dial CBV @10:15:59.02
CBV.dial SSV @10:15:59.82 Allowing 130s for 25.0ml of remaining 1325.0ml @10:15:59.83 Intake.select :open @10:16:02.00 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:16:02.02 SS.reconfigure SSinhale.with maxSpeed: 26 @10:16:02.07 SS.seek 25ml @10:17:09.33 Stroke completed -- waiting for pressure to equalize ... @10:17:15.06 Intake.select :close @10:17:15.22 SSV.dial :exhaust @10:17:16.02 Exhaust.select :open @10:17:16.14 SS.reconfigure SSexhale @10:17:16.18 SS.seek 0ml @10:17:25.16 Exhaust.select :close @10:17:25.28 SSV.dial CBV @10:17:25.30
CBV.dial SSV @10:17:26.10 Allowing 130s for 25.0ml of remaining 1300.0ml @10:17:26.11 Intake.select :open @10:17:28.28 Intake @31.9psia, vacuum from 20.0 to 24.0psi @10:17:28.30 SS.reconfigure SSinhale.with maxSpeed: 26 @10:17:28.35 SS.seek 25ml @10:18:35.57 Stroke completed -- waiting for pressure to equalize ... @10:18:41.27 Intake.select :close @10:18:41.43 SSV.dial :exhaust @10:18:42.23 Exhaust.select :open @10:18:42.35 SS.reconfigure SSexhale @10:18:42.40 SS.seek 0ml @10:18:51.44 Exhaust.select :close @10:18:51.56 SSV.dial CBV @10:18:51.58
CBV.dial SSV @10:18:52.38 Allowing 130s for 25.0ml of remaining 1275.0ml @10:18:52.39 Intake.select :open @10:18:54.57 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:18:54.58 SS.reconfigure SSinhale.with maxSpeed: 26 @10:18:54.63 SS.seek 25ml @10:20:01.97 Stroke completed -- waiting for pressure to equalize ... @10:20:08.27 Intake.select :close @10:20:08.42 SSV.dial :exhaust @10:20:09.23 Exhaust.select :open @10:20:09.35 SS.reconfigure SSexhale @10:20:09.40 SS.seek 0ml @10:20:18.49 Exhaust.select :close @10:20:18.61 SSV.dial CBV @10:20:18.63
CBV.dial SSV @10:20:19.43 Allowing 130s for 25.0ml of remaining 1250.0ml @10:20:19.95 Intake.select :open @10:20:22.16 Intake @32.1psia, vacuum from 20.0 to 24.0psi @10:20:22.18 SS.reconfigure SSinhale.with maxSpeed: 26 @10:20:22.23 SS.seek 25ml @10:21:29.50 Stroke completed -- waiting for pressure to equalize ... @10:21:35.89 Intake.select :close @10:21:36.05 SSV.dial :exhaust @10:21:36.85 Exhaust.select :open @10:21:36.97 SS.reconfigure SSexhale @10:21:37.02 SS.seek 0ml @10:21:46.12 Exhaust.select :close @10:21:46.24 SSV.dial CBV @10:21:46.26
CBV.dial SSV @10:21:47.07 Allowing 130s for 25.0ml of remaining 1225.0ml @10:21:47.08 Intake.select :open @10:21:49.25 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:21:49.27 SS.reconfigure SSinhale.with maxSpeed: 26 @10:21:49.32 SS.seek 25ml @10:22:56.79 Stroke completed -- waiting for pressure to equalize ... @10:23:03.72 Intake.select :close @10:23:03.87 SSV.dial :exhaust @10:23:04.68 Exhaust.select :open @10:23:04.80 SS.reconfigure SSexhale @10:23:04.85 SS.seek 0ml @10:23:13.99 Exhaust.select :close @10:23:14.11 SSV.dial CBV @10:23:14.13
CBV.dial SSV @10:23:14.93 Allowing 130s for 25.0ml of remaining 1200.0ml @10:23:14.94 Intake.select :open @10:23:17.11 Intake @32.1psia, vacuum from 20.0 to 24.0psi @10:23:17.13 SS.reconfigure SSinhale.with maxSpeed: 26 @10:23:17.18 SS.seek 25ml @10:24:04.39 Vacuum too high -- waiting up to 40s @10:24:06.20 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:24:06.22 SS.reconfigure SSinhale.with maxSpeed: 23 @10:24:06.27 SS.seek 25ml @10:24:29.40 Stroke completed -- waiting for pressure to equalize ... @10:24:36.25 Intake.select :close @10:24:36.41 SSV.dial :exhaust @10:24:37.22 Exhaust.select :open @10:24:37.34 SS.reconfigure SSexhale @10:24:37.38 SS.seek 0ml @10:24:46.46 Exhaust.select :close @10:24:46.58 SSV.dial CBV @10:24:46.60
CBV.dial SSV @10:24:47.40 Allowing 130s for 25.0ml of remaining 1175.0ml @10:24:47.41 Intake.select :open @10:24:49.58 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:24:49.60 SS.reconfigure SSinhale.with maxSpeed: 23 @10:24:49.65 SS.seek 25ml @10:26:05.75 Stroke completed -- waiting for pressure to equalize ... @10:26:13.32 Intake.select :close @10:26:13.48 SSV.dial :exhaust @10:26:14.29 Exhaust.select :open @10:26:14.41 SS.reconfigure SSexhale @10:26:14.46 SS.seek 0ml @10:26:23.67 Exhaust.select :close @10:26:23.79 SSV.dial CBV @10:26:23.81
CBV.dial SSV @10:26:24.62 Allowing 130s for 25.0ml of remaining 1150.0ml @10:26:24.63 Intake.select :open @10:26:26.80 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:26:26.82 SS.reconfigure SSinhale.with maxSpeed: 23 @10:26:26.87 SS.seek 25ml @10:27:27.67 Vacuum too high -- waiting up to 40s @10:27:29.74 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:27:29.76 SS.reconfigure SSinhale.with maxSpeed: 21 @10:27:29.81 SS.seek 25ml @10:27:46.59 Stroke completed -- waiting for pressure to equalize ... @10:27:53.51 Intake.select :close @10:27:53.66 SSV.dial :exhaust @10:27:54.46 Exhaust.select :open @10:27:55.11 SS.reconfigure SSexhale @10:27:55.17 SS.seek 0ml @10:28:04.29 Exhaust.select :close @10:28:04.41 SSV.dial CBV @10:28:04.44
CBV.dial SSV @10:28:05.23 Allowing 130s for 25.0ml of remaining 1125.0ml @10:28:05.24 Intake.select :open @10:28:07.42 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:28:07.44 SS.reconfigure SSinhale.with maxSpeed: 21 @10:28:07.49 SS.seek 25ml @10:29:30.95 Stroke completed -- waiting for pressure to equalize ... @10:29:39.16 Intake.select :close @10:29:39.32 SSV.dial :exhaust @10:29:40.12 Exhaust.select :open @10:29:40.24 SS.reconfigure SSexhale @10:29:40.29 SS.seek 0ml @10:29:49.43 Exhaust.select :close @10:29:49.55 SSV.dial CBV @10:29:49.57
CBV.dial SSV @10:29:50.37 Allowing 130s for 25.0ml of remaining 1100.0ml @10:29:50.38 Intake.select :open @10:29:52.55 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:29:52.57 SS.reconfigure SSinhale.with maxSpeed: 21 @10:29:52.62 SS.seek 25ml @10:30:45.55 Vacuum too high -- waiting up to 40s @10:30:47.61 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:30:47.63 SS.reconfigure SSinhale.with maxSpeed: 19 @10:30:47.68 SS.seek 25ml @10:31:22.17 Stroke completed -- waiting for pressure to equalize ... @10:31:30.30 Intake.select :close @10:31:30.45 SSV.dial :exhaust @10:31:31.26 Exhaust.select :open @10:31:31.38 SS.reconfigure SSexhale @10:31:31.42 SS.seek 0ml @10:31:40.53 Exhaust.select :close @10:31:40.65 SSV.dial CBV @10:31:40.67
CBV.dial SSV @10:31:41.48 Allowing 130s for 25.0ml of remaining 1075.0ml @10:31:41.49 Intake.select :open @10:31:43.66 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:31:43.68 SS.reconfigure SSinhale.with maxSpeed: 19 @10:31:43.73 SS.seek 25ml @10:33:11.91 Vacuum too high -- waiting up to 40s @10:33:14.26 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:33:14.27 SS.reconfigure SSinhale.with maxSpeed: 17 @10:33:14.32 SS.seek 25ml @10:33:19.44 Stroke completed -- waiting for pressure to equalize ... @10:33:27.63 Intake.select :close @10:33:27.79 SSV.dial :exhaust @10:33:28.59 Exhaust.select :open @10:33:28.71 SS.reconfigure SSexhale @10:33:28.76 SS.seek 0ml @10:33:37.76 Exhaust.select :close @10:33:37.88 SSV.dial CBV @10:33:37.90
CBV.dial SSV @10:33:38.71 Allowing 130s for 25.0ml of remaining 1050.0ml @10:33:38.72 Intake.select :open @10:33:40.90 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:33:40.91 SS.reconfigure SSinhale.with maxSpeed: 17 @10:33:40.96 SS.seek 25ml @10:35:24.31 Stroke completed -- waiting for pressure to equalize ... @10:35:35.65 Intake.select :close @10:35:35.80 SSV.dial :exhaust @10:35:36.61 Exhaust.select :open @10:35:36.73 SS.reconfigure SSexhale @10:35:37.33 SS.seek 0ml @10:35:46.45 Exhaust.select :close @10:35:46.57 SSV.dial CBV @10:35:46.59
CBV.dial SSV @10:35:47.42 Allowing 130s for 25.0ml of remaining 1025.0ml @10:35:47.43 Intake.select :open @10:35:49.60 Intake @32.1psia, vacuum from 20.0 to 24.0psi @10:35:49.62 SS.reconfigure SSinhale.with maxSpeed: 17 @10:35:49.67 SS.seek 25ml @10:37:05.46 Vacuum too high -- waiting up to 40s @10:37:08.03 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:37:08.05 SS.reconfigure SSinhale.with maxSpeed: 15 @10:37:08.10 SS.seek 25ml @10:37:39.14 Stroke completed -- waiting for pressure to equalize ... @10:37:49.82 Intake.select :close @10:37:49.97 SSV.dial :exhaust @10:37:50.78 Exhaust.select :open @10:37:50.90 SS.reconfigure SSexhale @10:37:50.94 SS.seek 0ml @10:38:00.28 Exhaust.select :close @10:38:00.40 SSV.dial CBV @10:38:00.42
CBV.dial SSV @10:38:01.23 Allowing 130s for 25.0ml of remaining 1000.0ml @10:38:01.24 Intake.select :open @10:38:03.41 Intake @31.9psia, vacuum from 20.0 to 24.0psi @10:38:03.43 SS.reconfigure SSinhale.with maxSpeed: 15 @10:38:03.48 SS.seek 25ml @10:39:58.63 Vacuum too high -- waiting up to 40s @10:40:01.45 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:40:01.47 SS.reconfigure SSinhale.with maxSpeed: 14 @10:40:01.51 SS.seek 25ml @10:40:03.84 Stroke completed -- waiting for pressure to equalize ... @10:40:11.32 SS.reconfigure SSconfig @10:40:11.38 SS.hold @10:40:11.51 Intake.select :close @10:40:11.67 ShallowSampler::Clogged in MAIN -- progress too slow after 525.0ml @10:40:11.68 SSV.dial :exhaust @10:40:12.48 Exhaust.select :open @10:40:12.60 SS.reconfigure SSexhale @10:40:12.64 SS.seek 0ml @10:40:22.01 Exhaust.select :close @10:40:22.12 Sampled 525.0ml @10:40:22.14 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:40:22.15 Intake.select :close @10:40:22.22 CSV.dial :waste @10:40:22.30 CS.seek 0ml @10:40:22.39 CSV.dial :air @10:40:22.43 SSV.dial CBV,avoiding: :exhaust @10:40:22.47
CBV.dial SSV @10:40:23.34 CS.seek 10ml @10:40:24.40
CTV.dial :air @10:40:25.17 delay 15 seconds @10:40:40.20 SSV.dial :spit,avoiding: :exhaust @10:40:41.57 CTV.dial CSV,avoiding: :intake @10:40:41.61
SS.seek 0ml @10:40:41.75 SSV.dial CBV,avoiding: :exhaust @10:40:46.87 CBV.dial between SSV and waste2 @10:40:47.29 CTV.dial CSV,avoiding: :air @10:40:47.32
CSV.dial CTV @10:40:47.82 CS.reconfigure CSpressurize @10:40:47.86 CS.seek 0ml @10:41:13.58 BubblePoint: Intake @70.0psia, Outlet @14.1psia @10:41:13.59 CBV.dial SSV @10:41:13.95 SS.reconfigure SSconfig @10:41:14.01 SS.seek 10ml @10:41:19.38 delay 15 seconds @10:41:34.53 Evacuated: Intake @35.5psia, Outlet @0.6psia @10:41:34.54 SSV.dial :spit,avoiding: :exhaust @10:41:35.90 SS.reconfigure SSexhale @10:41:35.96 SS.seek 0ml @10:41:40.86 SS.reconfigure SSfast @10:41:40.91 SS.seek 5ml @10:41:43.62 SSV.dial CBV,avoiding: :exhaust @10:41:43.66
CBV.dial SSV @10:41:45.03 CTV.dial :air,avoiding: :intake @10:41:46.41 CSV.dial CTV @10:41:46.46
SS.seek 10ml @10:41:47.46 CSV.dial :air @10:41:47.95 CS.reconfigure CSfast @10:41:48.00 CS.seek 0ml @10:41:48.90
SSV.dial :spit,avoiding: :exhaust @10:41:49.24 CS.coast @10:41:50.26
SS.reconfigure SSexhale @10:41:50.31 SS.seek 0ml @10:41:55.74 SS.reconfigure SSfast @10:41:55.79 SS.seek 5ml @10:41:58.51 SSV.dial CBV,avoiding: :exhaust @10:41:58.53
CBV.dial SSV @10:42:00.91 SSV.dial :spit,avoiding: :exhaust @10:42:02.26 SS.reconfigure SSconfig @10:42:02.31 SS.seek 0ml @10:42:05.44 SS.coast @10:42:05.48 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:42:05.49 Intake.select :close @10:42:05.57 CSV.dial :waste @10:42:05.70 SSV.dial CBV,avoiding: :exhaust @10:42:05.72
CBV.dial SSV @10:42:06.41 CS.seek 0ml @10:42:06.50 CSV.dial :air @10:42:07.09
CTV.dial :air @10:42:07.11 delay 15 seconds @10:42:07.26 CS.seek 10ml @10:42:22.13
SSV.dial :spit,avoiding: :exhaust @10:42:23.49 CTV.dial CSV,avoiding: :intake @10:42:23.53
SS.reconfigure SSexhale @10:42:23.63 SS.seek 0ml @10:42:23.70 SSV.dial CBV,avoiding: :exhaust @10:42:30.40 CBV.dial between SSV and waste2 @10:42:30.84 CSV.dial between air and CTV @10:42:31.17 CS.seek 8.264ml @10:42:36.14 CTV.dial CSV,avoiding: :air @10:42:36.16
CSV.dial CTV @10:42:36.48 CS.reconfigure CSpressurize @10:42:36.52 CS.seek 0ml @10:42:58.28 CSV.dial :air @10:42:58.73 CS.reconfigure CSfast @10:42:58.79 CS.seek 10ml @10:43:21.62 CSV.dial between air and CTV @10:43:21.96 CS.seek 3.056ml @10:43:38.26 CTV.dial CSV,avoiding: :air @10:43:38.28
CSV.dial CTV @10:43:38.62 CS.reconfigure CSpressurize @10:43:38.66 CS.seek 0ml @10:43:46.05 BubblePoint: Intake @69.2psia, Outlet @14.0psia @10:43:46.06 CBV.dial SSV @10:43:46.41 SS.reconfigure SSconfig @10:43:46.47 SS.seek 10ml @10:43:51.87 delay 15 seconds @10:44:07.01 Evacuated: Intake @40.2psia, Outlet @14.6psia @10:44:07.02 SSV.dial :spit,avoiding: :exhaust @10:44:08.37 SS.reconfigure SSexhale @10:44:08.42 SS.seek 0ml @10:44:13.32 SS.reconfigure SSfast @10:44:13.37 SS.seek 5ml @10:44:16.00 SSV.dial CBV,avoiding: :exhaust @10:44:16.04
CBV.dial SSV @10:44:17.39 CTV.dial :air,avoiding: :intake @10:44:18.75 CSV.dial CTV @10:44:18.80
SS.seek 10ml @10:44:19.80 CSV.dial :air @10:44:20.27 CS.reconfigure CSfast @10:44:20.32 CS.seek 0ml @10:44:21.25
SSV.dial :spit,avoiding: :exhaust @10:44:22.61 SS.reconfigure SSexhale @10:44:22.66 SS.seek 0ml @10:44:22.88 CS.coast @10:44:27.81
SS.reconfigure SSfast @10:44:27.87 SS.seek 5ml @10:44:30.41 SSV.dial CBV,avoiding: :exhaust @10:44:30.44
CBV.dial SSV @10:44:32.82 SSV.dial :spit,avoiding: :exhaust @10:44:34.16 SS.reconfigure SSconfig @10:44:34.21 SS.seek 0ml @10:44:37.35 SS.coast @10:44:37.39 Lysing: Adding 1.5ml lysis @10:44:37.40 CBV.dial between CSV and waste1,avoiding: :DBV @10:44:38.94 CTV.dial CSV @10:44:40.30 CSV.dial CSR @10:44:41.13 Pulling 1ml of CSR.lysis @10:44:41.14 CSR.select :lysis @10:44:41.26 CS.reconfigure CSconfig @10:44:41.31 CS.seek 1ml @10:44:44.82 delay 5 seconds @10:44:49.85 CSR.select :air @10:44:50.03 CS.seek 6.001ml @10:45:03.72 delay 2 seconds @10:45:05.74 CSV.dial :waste1 @10:45:07.05 CS.reconfigure CSfast @10:45:07.10 CS.seek :empty @10:45:21.19 CSV.dial CSR @10:45:22.56 Pulling 1.5ml of CSR.lysis @10:45:22.57 CSR.select :lysis @10:45:22.69 CS.reconfigure CSconfig @10:45:22.74 CS.seek 1.5ml @10:45:27.66 delay 5 seconds @10:45:32.69 CSR.select :air @10:45:32.87 CS.seek 4.501ml @10:45:41.47 delay 2 seconds @10:45:43.49 CSV.dial CTV @10:45:43.98 CS.seek 1.275ml @10:45:53.20 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @10:45:53.22 CH.seek 85C,8:00 @10:48:59.10 CH.hold 5:00,85C @10:53:59.28 Collection Puck Heater at 85C after lysing @10:53:59.30 CS.seek 0ml @10:54:03.64 CTV.dial between spare and air,avoiding: :intake @10:54:04.72 CSV.dial :air @10:54:05.21 CS.seek 5ml @10:54:19.05 CSV.dial CTV @10:54:19.53 delay 2 seconds @10:54:21.55 CSV.dial :air @10:54:22.03 delay 2 seconds @10:54:24.05 CSV.dial CTV @10:54:24.53 delay 2 seconds @10:54:26.55 CSV.dial :air @10:54:27.03 delay 2 seconds @10:54:29.06 CS.seek 0ml @10:54:42.86 Recovering Lysate into Collection syringe @10:54:42.87 CSV.dial CBV @10:54:43.91 CBV.dial CSV @10:54:44.32 CS.seek 5ml @10:54:58.05 delay 5 seconds @10:55:03.08 CTV.dial :air @10:55:03.63 CS.seek 7ml @10:55:09.77 delay 5 seconds @10:55:14.80 CSV.dial :air2 @10:55:15.28 CS.seek 10ml @10:55:23.99 CSV.dial between air and CTV,avoiding: CBV @10:55:25.16 Lysing Complete @10:55:25.24 Storing sh1 puck in Collection Clamp to tube 1 @10:55:25.43 SC.reconfigure SCtight @10:55:25.48 SC.seek 1 @10:55:27.61 SC.reconfigure SCloose @10:55:27.84 Elbow.reconfigure TightElbow @10:55:27.89 Elbow.seek 1 @10:55:29.88 Forearm.reconfigure TightForearm @10:55:29.93 Forearm.seek 1 + 700 counts @10:55:30.93 SE.reconfigure SEpick @10:55:31.02 SE.seek beamBreak @10:55:32.92 SE.configure SEconfig @10:55:33.09 Elbow.reconfigure LimpElbow @10:55:33.14 Forearm.reconfigure LooseForearm @10:55:33.18 Forearm.seek 1 @10:55:33.50 Elbow.hold @10:55:33.57 Elbow.reconfigure TightElbow @10:55:33.68 SE.seek 11.09 Pucks below Top Plate @10:55:34.11 Forearm.force -20 @10:55:34.15 Forearm.seek 1 + 319 counts @10:55:35.09 Forearm.reconfigure TightForearm @10:55:35.14 Forearm.seek 1 + 700 counts @10:55:35.53 SE.seek 12.09 Pucks below Top Plate @10:55:36.18 Forearm.reconfigure LooseForearm @10:55:36.23 Forearm.seek :retract @10:55:37.17 Elbow.seek CC @10:55:38.80 Elbow.reconfigure LooseElbow @10:55:38.91 Forearm.seek CC @10:55:39.97 Hand.close @10:55:40.60 CC.seek :open @10:55:45.09 Forearm.seek :retract @10:55:46.14 Elbow.reconfigure TightElbow @10:55:46.19 Elbow.seek 1 @10:55:48.15 Elbow.reconfigure LimpElbow @10:55:48.21 Forearm.seek 1 @10:55:49.24 Elbow.hold @10:55:49.31 Elbow.reconfigure TightElbow @10:55:49.36 Hand.open @10:55:50.11 Forearm.force -20 @10:55:50.15 Forearm.seek 1 + 348 counts @10:55:50.49 Forearm.reconfigure TightForearm @10:55:50.54 Forearm.seek 1 + 700 counts @10:55:50.83 SE.seek :down @10:55:51.99
Forearm.reconfigure LooseForearm @10:55:52.04 Forearm.seek :retract @10:55:52.54 SE.coast @10:55:52.94
SC.coast @10:55:52.98 Tranferring lysate to PRV @10:55:52.99 CSV.dial :air @10:55:53.32 CS.seek 10ml @10:55:53.39 CSV.dial between air and CTV @10:55:53.77 AIV.dial :bypass @10:55:53.88 Beginning samplePassC @10:55:53.89 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:55:53.97 Hand.open @10:55:54.35 Elbow.seek FlushPuck::Garage @10:55:56.37 Elbow.reconfigure LooseElbow @10:55:56.48 Forearm.seek :garage @10:55:57.49 Hand.close @10:55:58.23 Forearm.seek :retract @10:55:59.22 Elbow.reconfigure TightElbow @10:55:59.27 Elbow.seek CC @10:56:02.17 Elbow.reconfigure LooseElbow @10:56:02.27 Forearm.reconfigure TightForearm @10:56:02.88 Forearm.seek CC @10:56:04.24 CC.seek :closed @10:56:08.52 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.91 @10:56:08.59 Hand.open @10:56:09.29 Forearm.reconfigure LooseForearm @10:56:09.34 Forearm.seek :retract @10:56:10.33 AWV.select :air @10:56:10.34 AIV.dial :bypass @10:56:10.35 PSV.dial PSR @10:56:11.76 Pulling 1.5ml of PSR.flush @10:56:11.77 PSR.select :flush @10:56:11.90 PS.seek 1.5ml @10:56:28.00 delay 5 seconds @10:56:33.03 PSV.dial :waste1 @10:56:33.51 PS.seek 0ml @10:56:49.59 PSV.dial PSR @10:56:50.06 PSR.select :air @10:56:50.18 PS.seek 2.5ml @10:57:16.28 PRV.dial CBV @10:57:16.31
CBV.dial PRV @10:57:17.18 PSV.dial :mixloop @10:57:18.32 CTV.dial :air,avoiding: :intake @10:57:18.33 PTV.dial :PRVmixing @10:57:18.43 PS.seek 0ml @10:57:44.62 PSV.dial :air @10:57:45.10 PS.seek 2.5ml @10:58:11.28 PSV.dial :mixloop @10:58:11.74 PS.seek 0ml @10:58:37.81 PSV.dial :air @10:58:38.27 PS.seek 2.5ml @10:59:04.37 PSV.dial :mixloop @10:59:04.84 PS.seek 0ml @10:59:30.90 Garaging FlushPuck in Collection Clamp @10:59:30.98 Elbow.reconfigure TightElbow @10:59:31.03 Elbow.seek CC @10:59:31.30 Elbow.reconfigure LooseElbow @10:59:31.41 Forearm.seek CC @10:59:32.43 Hand.close @10:59:33.10 CC.seek :open @10:59:37.46 Forearm.seek :retract @10:59:38.49 Elbow.reconfigure TightElbow @10:59:38.54 Elbow.seek :garage @10:59:41.74 Elbow.reconfigure LooseElbow @10:59:41.84 Forearm.reconfigure TightForearm @10:59:41.89 Forearm.seek :garage @10:59:43.06 Hand.open @10:59:43.80 Forearm.reconfigure LooseForearm @10:59:43.85 Forearm.seek :retract @10:59:44.87 Connecting PS to CS to transfer sample @10:59:44.88 CBV.dial AIV @10:59:44.89 AIV.dial :bypass @10:59:44.90 PSV.dial :mixloop @10:59:44.92 PTV.dial :PRVmixing @10:59:44.93 PRV.dial AIV @10:59:45.42 CTV.dial :air,avoiding: :intake @10:59:45.52 Loading lyfil puck from tube 2 into Collection Clamp @10:59:45.78 SC.reconfigure SCtight @10:59:45.83 SC.seek 2 @10:59:48.01 SC.reconfigure SCloose @10:59:48.25 Elbow.reconfigure TightElbow @10:59:48.30 Elbow.seek 2 @10:59:50.40 Forearm.reconfigure TightForearm @10:59:50.45 Forearm.seek 2 + 700 counts @10:59:51.49 SE.reconfigure SEpick @10:59:51.57 SE.seek beamBreak @10:59:53.87 SE.configure SEconfig @10:59:54.03 Elbow.seek 2 @10:59:54.33 Forearm.seek 2 + 300 counts @10:59:54.57 Forearm.reconfigure LooseForearm @10:59:54.61 Hand.close @10:59:55.25 SE.seek :down @10:59:55.48
Forearm.seek :retract @10:59:56.45 Elbow.seek CC @10:59:57.34 SE.coast @10:59:58.03
Elbow.reconfigure LooseElbow @10:59:58.14 Forearm.reconfigure TightForearm @10:59:58.19 Forearm.seek CC @10:59:59.55 CC.seek :closed @11:00:03.80 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.02 @11:00:03.81 Hand.open @11:00:04.55 Forearm.reconfigure LooseForearm @11:00:04.60 Forearm.seek :retract @11:00:05.59 SC.coast @11:00:05.63 CTV.dial CSV,avoiding: :intake @11:00:06.99 CSV.dial CTV @11:00:07.30 CS.seek 5ml @11:00:21.40 delay 1:20 @11:01:41.44 CS.seek 2.5ml @11:01:48.87 delay 40 seconds @11:02:28.91 CS.seek 0ml @11:02:36.53 delay 1:00 @11:03:36.56 PSV.dial :air @11:03:37.03 PS.seek 2.5ml @11:04:03.13 PSV.dial :mixloop @11:04:03.58 delay 3 seconds @11:04:06.60 PSV.dial :air @11:04:07.07 delay 2 seconds @11:04:09.09 PSV.dial :mixloop @11:04:09.55 delay 3 seconds @11:04:12.58 PSV.dial :waste1 @11:04:13.92 PS.seek :empty @11:04:40.03 CBV.dial :waste1 @11:04:40.53 delay 5 seconds @11:04:45.56 CBV.dial AIV @11:04:46.04 delay 10 seconds @11:04:56.07 CBV.dial :waste1 @11:04:56.54 delay 10 seconds @11:05:06.57 CBV.dial AIV @11:05:07.06 delay 10 seconds @11:05:17.65 CTV.dial CSV,avoiding: :intake @11:05:17.75 CBV.dial between AIV and waste1 @11:05:18.11 CSV.dial :air @11:05:18.60 CS.seek 10ml @11:05:45.12 CSV.dial CTV @11:05:45.60 CS.seek 0ml @11:06:12.16 CBV.dial :waste1 @11:06:12.50 delay 10 seconds @11:06:22.53 CTV.dial :air,avoiding: :intake @11:06:23.98 Storing lyfil puck in Collection Clamp to tube 1 @11:06:24.17 SC.reconfigure SCtight @11:06:24.21 SC.seek 1 @11:06:26.36 SC.reconfigure SCloose @11:06:26.58 Elbow.reconfigure TightElbow @11:06:26.63 Elbow.seek 1 @11:06:28.65 Forearm.reconfigure TightForearm @11:06:28.71 Forearm.seek 1 + 700 counts @11:06:29.81 SE.reconfigure SEpick @11:06:29.89 SE.seek beamBreak @11:06:31.83 SE.configure SEconfig @11:06:31.99 Elbow.reconfigure LimpElbow @11:06:32.04 Forearm.reconfigure LooseForearm @11:06:32.09 Forearm.seek 1 @11:06:32.40 Elbow.hold @11:06:32.47 Elbow.reconfigure TightElbow @11:06:32.58 SE.seek 12.09 Pucks below Top Plate @11:06:33.15 Forearm.force -20 @11:06:33.19 Forearm.seek 1 + 322 counts @11:06:33.54 Forearm.reconfigure TightForearm @11:06:33.59 Forearm.seek 1 + 700 counts @11:06:33.81 SE.seek 13.09 Pucks below Top Plate @11:06:34.46 Forearm.reconfigure LooseForearm @11:06:34.50 Forearm.seek :retract @11:06:35.43 Elbow.seek CC @11:06:37.07 Elbow.reconfigure LooseElbow @11:06:37.18 Forearm.seek CC @11:06:38.21 Hand.close @11:06:38.88 CC.seek :open @11:06:43.41 Forearm.seek :retract @11:06:44.46 Elbow.reconfigure TightElbow @11:06:44.51 Elbow.seek 1 @11:06:46.47 Elbow.reconfigure LimpElbow @11:06:46.53 Forearm.seek 1 @11:06:47.52 Elbow.hold @11:06:47.59 Elbow.reconfigure TightElbow @11:06:47.64 Hand.open @11:06:48.37 Forearm.force -20 @11:06:48.41 Forearm.seek 1 + 350 counts @11:06:48.73 Forearm.reconfigure TightForearm @11:06:48.78 Forearm.seek 1 + 700 counts @11:06:49.75 SE.seek :down @11:06:50.90
Forearm.reconfigure LooseForearm @11:06:50.95 Forearm.seek :retract @11:06:51.24 SE.coast @11:06:51.87
SC.coast @11:06:51.91 PSV.dial :air @11:06:53.00 PS.seek 0.499ml @11:06:58.85 delay 3 seconds @11:07:01.87 PSV.dial :mixloop @11:07:02.32 PRV.dial :air @11:07:02.80 delay 3 seconds @11:07:05.82 PRV.dial CBV @11:07:06.29 CBV.dial AIV @11:07:06.78 PRV.dial between CBV and air @11:07:07.12 PRV.dial :air @11:07:07.51 PS.reconfigure PSslow @11:07:07.56 PS.seek 0.599ml @11:07:10.16 PRV.dial :waste2 @11:07:10.91 PS.reconfigure PSconfig @11:07:10.96 PS.seek :empty @11:07:17.94 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:07:18.02 Hand.open @11:07:18.37 Elbow.seek FlushPuck::Garage @11:07:20.41 Elbow.reconfigure LooseElbow @11:07:20.52 Forearm.seek :garage @11:07:21.55 Hand.close @11:07:22.25 Forearm.seek :retract @11:07:23.28 Elbow.reconfigure TightElbow @11:07:23.33 Elbow.seek CC @11:07:26.29 Elbow.reconfigure LooseElbow @11:07:26.39 Forearm.reconfigure TightForearm @11:07:26.45 Forearm.seek CC @11:07:27.84 CC.seek :closed @11:07:32.09 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.91 @11:07:32.17 Hand.open @11:07:32.81 Forearm.reconfigure LooseForearm @11:07:32.90 Forearm.seek :retract @11:07:34.01 Clearing PML for modified lysate @11:07:34.02 CTV.dial :air,avoiding: :intake @11:07:34.03 PRV.dial :waste2 @11:07:34.04 PTV.dial :PRV,avoiding: :puck @11:07:34.80 delay 2 seconds @11:07:36.82 PTV.dial :PRVmixing,avoiding: :puck @11:07:37.60 delay 2 seconds @11:07:39.62 PSV.dial :PML @11:07:39.63 delay 2 seconds @11:07:41.66 PRV.dial :air @11:07:42.43 delay 2 seconds @11:07:44.51 PS.seek 1.499ml @11:08:00.57 delay 5 seconds @11:08:06.16 PS.seek 2.5ml @11:08:17.23 PSV.dial :air @11:08:17.71 PS.seek 0ml @11:08:43.91 mixing lysate with speDil @11:08:43.92 PSV.dial :PML @11:08:44.38 CBV.dial :PRV @11:08:44.39 PRV.dial :air @11:08:44.41 delay 2 seconds @11:08:46.43 PRV.dial :speDil @11:08:46.91 delay 2 seconds @11:08:49.00 PS.reconfigure PSslow1 @11:08:49.05 PS.seek 0.225ml @11:09:12.38 delay 5 seconds @11:09:17.41 PRV.dial CBV @11:09:18.18 delay 2 seconds @11:09:20.26 PS.seek 0.475ml @11:09:45.95 delay 5 seconds @11:09:50.98 PRV.dial :air @11:09:51.46 delay 2 seconds @11:09:53.49 PS.seek 0.965ml @11:10:43.38 delay 10 seconds @11:10:53.41 PRV.dial CBV @11:10:53.89 CTV.dial :air,avoiding: :intake @11:10:53.90 PSV.dial :air @11:10:54.37 PS.reconfigure PSconfig @11:10:54.43 PS.seek 2.5ml @11:11:10.85 delay 3 seconds @11:11:13.87 PSV.dial PTV @11:11:14.62 PTV.dial :PRV,avoiding: :puck @11:11:15.41 PS.seek 0ml @11:11:41.54 PSV.dial :air @11:11:42.30 PS.seek 2.5ml @11:12:08.51 delay 3 seconds @11:12:11.53 PSV.dial PTV @11:12:12.29 PTV.dial :PRV,avoiding: :puck @11:12:12.31 PS.seek 0ml @11:12:38.39 Pushing modified lysate into AIV Injection Loop @11:12:38.40 CBV.dial AIV @11:12:38.41 AIV.dial :bypass @11:12:38.42 PRV.dial CBV @11:12:38.44 PSV.dial :air @11:12:39.19 PS.seek 2.5ml @11:13:05.39 delay 3 seconds @11:13:08.41 PSV.dial :PML @11:13:08.87 PTV.dial :PRVmixing,avoiding: :puck @11:13:09.72 PS.reconfigure PSslow1 @11:13:09.77 PS.seek 1.74ml @11:14:27.14 delay 5 seconds @11:14:32.17 AIV.dial :sample @11:14:32.76 AWV.select :waste @11:14:32.93 PS.reconfigure PSslow2 @11:14:32.98 PS.seek 1.22ml @11:16:19.92 delay 15 seconds @11:16:34.95 AIV.dial :bypass @11:16:35.53 PS.reconfigure PSconfig @11:16:35.58 PS.seek 0ml @11:16:48.73 PSV.dial :air @11:16:49.20 PS.seek 2.5ml @11:17:15.30 PSV.dial :PML @11:17:15.78 PS.seek 0ml @11:17:41.89 AIV.dial :coilToMFB @11:17:41.90 AWV.select :air @11:17:42.07 Extracting Nucleic Acid from AIV @11:17:42.10 SPE.hold 2:46:40,55C @11:17:42.13
Beginning samplePassD @11:17:42.21 ASV.dial :SPEdelivery @11:17:42.30
AWV.select :air @11:17:42.31 AIV.dial :bypass @11:17:42.33 PSV.dial PSR @11:17:42.61 delay 20 seconds @11:17:43.50
Pulling 1.5ml of PSR.killMFB @11:17:43.51 PSR.select :killMFB @11:17:43.64 PS.seek 1.5ml @11:17:59.67 delay 5 seconds @11:18:02.64 primeLysateINT @11:18:02.65 AWV.select :air @11:18:02.66 AIV.dial :coilToMFB @11:18:03.23 ATV.dial AIV @11:18:04.69
PSV.dial :waste1 @11:18:04.92 AS.reconfigure ASslow1 @11:18:04.97 AS.seek 0.299ml @11:18:05.29
PS.seek 0ml @11:18:21.33 PSV.dial PSR @11:18:21.81 PSR.select :air @11:18:21.94 PS.seek 2.5ml @11:18:48.05 PRV.dial CBV @11:18:48.08
CBV.dial PRV @11:18:48.09 PSV.dial :mixloop @11:18:49.19 CTV.dial :air,avoiding: :intake @11:18:49.20 PTV.dial :PRVmixing @11:18:49.22 PS.seek 0ml @11:19:05.65 delay 10 seconds @11:19:15.46
PSV.dial PSR @11:19:15.67 ATV.dial :air2 @11:19:16.50 AS.seek 0.329ml @11:19:16.63
Pulling 1.5ml of PSR.flush @11:19:16.64 PSR.select :flush @11:19:16.77 PS.seek 1.5ml @11:19:22.85 ATV.dial :waste2 @11:19:23.41 AS.reconfigure ASnorm @11:19:23.46 AS.seek :empty @11:19:32.89
delay 5 seconds @11:19:37.92 PSV.dial :waste1 @11:19:38.42 PS.seek 0ml @11:19:40.44 Lysate primed! @11:19:40.45 AFV.select :holdingCoil @11:19:40.46 ATV.dial :air2 @11:19:41.14 AS.reconfigure ASslow1 @11:19:41.19 AS.seek 0.069ml @11:19:54.39
PSV.dial PSR @11:19:54.88 PSR.select :air @11:19:55.01 PS.seek 2.5ml @11:19:55.57 delay 3 seconds @11:19:58.59 ATV.dial :AIV @11:19:59.47 AS.seek 0.469ml @11:20:21.18 PRV.dial CBV @11:20:21.21
CBV.dial PRV @11:20:21.22 PSV.dial :mixloop @11:20:22.30 CTV.dial :air,avoiding: :intake @11:20:22.31 PTV.dial :PRVmixing @11:20:22.33 PS.seek 0ml @11:20:48.46 PSV.dial PSR @11:20:49.60 Pulling 1.5ml of PSR.flush @11:20:49.61 PSR.select :flush @11:20:49.74 PS.seek 1.5ml @11:21:05.71 delay 5 seconds @11:21:10.74 PSV.dial :waste1 @11:21:11.23 PS.seek 0ml @11:21:20.18 delay 5 seconds @11:21:25.26 Solid Phase Extraction Heater at 47.4C @11:21:25.28 ATV.dial ASV @11:21:25.68 ASV.dial :SPEdelivery @11:21:25.76 AS.seek 0.041ml @11:21:27.18
PSV.dial PSR @11:21:27.66 PSR.select :air @11:21:27.79 PS.seek 2.5ml @11:21:53.86 PRV.dial CBV @11:21:53.89
CBV.dial PRV @11:21:53.90 PSV.dial :mixloop @11:21:54.96 CTV.dial :air,avoiding: :intake @11:21:54.97 PTV.dial :PRVmixing @11:21:54.99 PS.seek 0ml @11:22:21.33 PSV.dial PSR @11:22:22.44 Pulling 1.5ml of PSR.flush @11:22:22.45 PSR.select :flush @11:22:22.58 PS.seek 1.5ml @11:22:38.65 delay 5 seconds @11:22:43.68 PSV.dial :waste1 @11:22:44.16 PS.seek 0ml @11:22:52.10 delay 10 seconds @11:23:00.24
PSV.dial PSR @11:23:01.38 PSR.select :air @11:23:01.51 PS.seek 2.5ml @11:23:02.12 rinseLysateLineINT @11:23:02.13 AFV.select :carrierSolution @11:23:02.24 delay 2 seconds @11:23:04.27 AS.reconfigure ASconfig @11:23:04.32 AS.seek 1ml @11:23:27.62 PRV.dial CBV @11:23:27.65
CBV.dial PRV @11:23:27.67 PSV.dial :mixloop @11:23:28.71 CTV.dial :air,avoiding: :intake @11:23:28.72 PTV.dial :PRVmixing @11:23:28.74 PS.reconfigure PSslow @11:23:28.79 PS.seek 1.2ml @11:23:55.59 PSV.dial :waste1 @11:23:56.93 PS.reconfigure PSconfig @11:23:56.99 PS.seek 0ml @11:23:59.74 delay 1 second @11:24:00.76 AFV.select :holdingCoil @11:24:00.87 delay 1 second @11:24:01.90 ATV.dial AIV @11:24:02.43 AIV.dial :coilToMFB @11:24:02.44 AWV.select :waste @11:24:02.56 AS.reconfigure ASslow0 @11:24:02.60 AS.seek 0ml @11:24:10.02
PSV.dial :air @11:24:11.06 PS.seek 2.5ml @11:24:37.18 PSV.dial :mixloop @11:24:37.63 PS.reconfigure PSslow @11:24:37.68 PS.seek 0ml @11:25:28.74 PSV.dial :air @11:25:29.20 PS.reconfigure PSconfig @11:25:29.25 PS.seek 2.5ml @11:25:43.51 ATV.dial :air2 @11:25:44.16 AS.reconfigure ASslow1 @11:25:44.21 AS.seek 0.05ml @11:25:54.54 ATV.dial :AIV @11:25:55.33 AS.seek 0.01ml @11:25:55.41
PSV.dial :mixloop @11:25:55.88 PS.reconfigure PSslow @11:25:55.92 PS.seek 0ml @11:26:03.65 ATV.dial :waste @11:26:05.21 AS.seek :empty @11:26:07.65 ethanol wash @11:26:07.66 ATV.dial :air2 @11:26:08.89 AS.reconfigure ASslow2 @11:26:08.94 AS.seek 0.009ml @11:26:19.50 delay 3 seconds @11:26:22.52 Pulling 0.4ml of Ethanol Wash @11:26:22.53 ATV.dial :ethanol @11:26:23.35 AS.reconfigure ASslow1 @11:26:23.40 AS.seek 0.409ml @11:26:46.93
Cleaning Collection Syringe @11:26:46.94 CSV.dial CSR @11:26:47.46 Pulling 4ml of CSR.flush @11:26:47.47 CSR.select :flush @11:26:47.60 CS.seek 4ml @11:26:58.78 delay 5 seconds @11:27:03.81 CSR.select :air @11:27:03.94 CS.seek 10ml @11:27:20.24 CSV.dial CTV @11:27:20.28
CTV.dial CSV @11:27:21.71 CBV.dial :waste1 @11:27:22.21 CS.seek 0ml @11:27:44.14 delay 3 seconds @11:27:47.17 ATV.dial :air1 @11:27:47.54 delay 3 seconds @11:27:48.65
CSV.dial CSR @11:27:49.19 Pulling 4ml of CSR.flush @11:27:49.20 CSR.select :flush @11:27:49.33 CS.seek 4ml @11:27:50.62 AS.seek 0.434ml @11:27:55.90 delay 3 seconds @11:27:58.92 ATV.dial :ethanol @11:27:59.50 AS.seek 0.419ml @11:28:00.50
delay 5 seconds @11:28:02.78 delay 3 seconds @11:28:05.53
CSR.select :air @11:28:05.65 CS.seek 10ml @11:28:05.80 ATV.dial ASV @11:28:07.66 AS.seek 0.092ml @11:28:21.96 CSV.dial CTV @11:28:21.99
CTV.dial CSV @11:28:22.48 CBV.dial :waste1 @11:28:22.50 CS.seek 0ml @11:28:49.07 Garaging FlushPuck in Collection Clamp @11:28:49.14 Elbow.reconfigure TightElbow @11:28:49.19 Elbow.seek CC @11:28:49.47 Elbow.reconfigure LooseElbow @11:28:49.57 Forearm.seek CC @11:28:50.58 Hand.close @11:28:51.15 CC.seek :open @11:28:55.53 Forearm.seek :retract @11:28:56.55 Elbow.reconfigure TightElbow @11:28:56.60 Elbow.seek :garage @11:28:59.82 Elbow.reconfigure LooseElbow @11:28:59.93 Forearm.reconfigure TightForearm @11:28:59.98 Forearm.seek :garage @11:29:01.11 Hand.open @11:29:01.85 Forearm.reconfigure LooseForearm @11:29:01.90 Forearm.seek :retract @11:29:02.92 Whole Cell Archive sampling 1500ml @11:29:02.94 Loading wcr puck from tube 2 into Collection Clamp @11:29:03.20 SC.reconfigure SCtight @11:29:03.25 SC.seek 2 @11:29:05.42 SC.reconfigure SCloose @11:29:05.66 Elbow.reconfigure TightElbow @11:29:05.71 Elbow.seek 2 @11:29:07.73 Forearm.reconfigure TightForearm @11:29:07.78 Forearm.seek 2 + 700 counts @11:29:08.85 SE.reconfigure SEpick @11:29:08.93 SE.seek beamBreak @11:29:11.31 SE.configure SEconfig @11:29:11.49 Elbow.seek 2 @11:29:11.69 Forearm.seek 2 + 300 counts @11:29:11.91 Forearm.reconfigure LooseForearm @11:29:11.96 Hand.close @11:29:12.55 SE.seek :down @11:29:12.79 Forearm.seek :retract @11:29:13.67 delay 3 seconds @11:29:13.73 Elbow.seek CC @11:29:14.74 SE.coast @11:29:15.43 Elbow.reconfigure LooseElbow @11:29:15.54 Forearm.reconfigure TightForearm @11:29:15.59 Forearm.seek CC @11:29:16.69 ATV.dial :waste2 @11:29:17.62 AS.reconfigure ASconfig @11:29:17.69 CC.seek :closed @11:29:17.85 AS.seek :empty @11:29:21.98 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.05 @11:29:21.99 Hand.open @11:29:22.74 Forearm.reconfigure LooseForearm @11:29:22.79 Forearm.seek :retract @11:29:23.50 ATV.dial :air2 @11:29:23.80 SC.coast @11:29:23.84 collectSample 1500ml w/bubble point of 55psi @11:29:23.86 Priming 7ml @11:29:23.88 Intake.select :close @11:29:23.89 Exhaust.select :close @11:29:23.90 SS.hold @11:29:24.04 AS.reconfigure ASslow1 @11:29:24.11 AS.seek 1ml @11:29:24.27 Calibrating pressure sensors @11:29:24.28 CTV.dial :air @11:29:25.64 SSV.dial :spit,avoiding: :exhaust @11:29:25.66 SS.reconfigure SSfast @11:29:25.71 SS.seek 5ml @11:29:28.32 SSV.dial CBV @11:29:28.34 CBV.dial SSV @11:29:29.81 delay 3.8 seconds @11:29:33.75 SSV.dial :spit @11:29:35.14 SS.seek 0ml @11:29:37.92 SSV.dial CBV @11:29:39.28 CTV.dial :intake @11:29:40.06 SSV.dial CBV @11:29:40.09 CBV.dial SSV @11:29:40.11 Allowing 40s for 7.0ml of remaining 7.0ml @11:29:40.12 Intake.select :open @11:29:42.30 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:29:42.32 SS.reconfigure SSinhale.with maxSpeed: 150 @11:29:42.37 SS.seek 7ml @11:29:48.03 Stroke completed -- waiting for pressure to equalize ... @11:30:00.67 Intake.select :close @11:30:00.83 SSV.dial :exhaust @11:30:02.24 Exhaust.select :open @11:30:02.36 SS.reconfigure SSexhale @11:30:02.41 SS.seek 0ml @11:30:06.02 Exhaust.select :close @11:30:06.13 Sampling 1500.0ml @11:30:06.15 Intake.select :close @11:30:06.16 Exhaust.select :close @11:30:06.17 SS.reconfigure SSconfig @11:30:06.22 SS.hold @11:30:06.45 CTV.dial :intake @11:30:06.47 SSV.dial CBV @11:30:06.50 CBV.dial SSV @11:30:07.31 Allowing 130s for 25.0ml of remaining 1500.0ml @11:30:07.33 Intake.select :open @11:30:09.51 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:30:09.53 SS.reconfigure SSinhale.with maxSpeed: 150 @11:30:09.58 SS.seek 25ml @11:30:13.57 Vacuum too high -- waiting up to 40s @11:30:15.96 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:30:15.98 SS.reconfigure SSinhale.with maxSpeed: 135 @11:30:16.04 SS.seek 25ml @11:30:18.80 Vacuum too high -- waiting up to 40s @11:30:21.16 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:30:21.18 SS.reconfigure SSinhale.with maxSpeed: 122 @11:30:21.22 SS.seek 25ml @11:30:24.08 Vacuum too high -- waiting up to 40s @11:30:26.44 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:30:26.46 SS.reconfigure SSinhale.with maxSpeed: 110 @11:30:26.50 SS.seek 25ml @11:30:29.21 Vacuum too high -- waiting up to 40s @11:30:31.55 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:30:32.15 SS.reconfigure SSinhale.with maxSpeed: 99 @11:30:32.22 SS.seek 25ml @11:30:35.70 Vacuum too high -- waiting up to 40s @11:30:37.85 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:30:37.87 SS.reconfigure SSinhale.with maxSpeed: 89 @11:30:37.92 SS.seek 25ml @11:30:40.63 Vacuum too high -- waiting up to 40s @11:30:42.76 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:30:42.78 SS.reconfigure SSinhale.with maxSpeed: 80 @11:30:42.83 SS.seek 25ml @11:30:46.30 Vacuum too high -- waiting up to 40s @11:30:48.42 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:30:48.44 SS.reconfigure SSinhale.with maxSpeed: 72 @11:30:48.49 SS.seek 25ml @11:30:51.91 Stroke completed -- waiting for pressure to equalize ... @11:30:57.01 Intake.select :close @11:30:57.17 SSV.dial :exhaust @11:30:57.97 Exhaust.select :open @11:30:58.09 SS.reconfigure SSexhale @11:30:58.14 SS.seek 0ml @11:31:06.88 Exhaust.select :close @11:31:07.00 SSV.dial CBV @11:31:07.02 CBV.dial SSV @11:31:07.85 Allowing 130s for 25.0ml of remaining 1475.0ml @11:31:07.86 Intake.select :open @11:31:10.04 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:31:10.06 SS.reconfigure SSinhale.with maxSpeed: 72 @11:31:10.11 SS.seek 25ml @11:31:15.41 Vacuum too high -- waiting up to 40s @11:31:17.60 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:31:17.62 SS.reconfigure SSinhale.with maxSpeed: 65 @11:31:17.67 SS.seek 25ml @11:31:22.76 Vacuum too high -- waiting up to 40s @11:31:24.61 SS.reconfigure SSinhale.with maxSpeed: 59 @11:31:24.67 SS.seek 25ml @11:31:30.47 Vacuum too high -- waiting up to 40s @11:31:32.34 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:31:32.36 SS.reconfigure SSinhale.with maxSpeed: 53 @11:31:32.41 SS.seek 25ml @11:31:39.54 Vacuum too high -- waiting up to 40s @11:31:41.39 SS.reconfigure SSinhale.with maxSpeed: 48 @11:31:41.44 SS.seek 25ml @11:31:50.95 Vacuum too high -- waiting up to 40s @11:31:52.54 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:31:52.56 SS.reconfigure SSinhale.with maxSpeed: 43 @11:31:52.61 SS.seek 25ml @11:31:54.27 Stroke completed -- waiting for pressure to equalize ... @11:31:58.13 Intake.select :close @11:31:58.29 SSV.dial :exhaust @11:31:59.10 Exhaust.select :open @11:31:59.22 SS.reconfigure SSexhale @11:31:59.27 SS.seek 0ml @11:32:08.35 Exhaust.select :close @11:32:08.47 SSV.dial CBV @11:32:08.49 CBV.dial SSV @11:32:09.30 Allowing 130s for 25.0ml of remaining 1450.0ml @11:32:09.31 Intake.select :open @11:32:11.50 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:32:11.52 SS.reconfigure SSinhale.with maxSpeed: 43 @11:32:11.57 SS.seek 25ml @11:32:45.70 delay 5 seconds @11:32:46.91 Vacuum too high -- waiting up to 40s @11:32:48.44 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:32:48.46 SS.reconfigure SSinhale.with maxSpeed: 39 @11:32:48.51 SS.seek 25ml @11:32:50.73 ATV.dial ASV @11:32:51.67 ASV.dial :SPEdelivery @11:32:51.69 AS.seek 0.02ml @11:32:54.99 Stroke completed -- waiting for pressure to equalize ... @11:32:58.87 Intake.select :close @11:32:59.03 SSV.dial :exhaust @11:32:59.83 Exhaust.select :open @11:32:59.95 SS.reconfigure SSexhale @11:33:00.00 SS.seek 0ml @11:33:09.02 Exhaust.select :close @11:33:09.14 SSV.dial CBV @11:33:09.16 CBV.dial SSV @11:33:09.97 Allowing 130s for 25.0ml of remaining 1425.0ml @11:33:09.98 Intake.select :open @11:33:12.17 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:33:12.19 SS.reconfigure SSinhale.with maxSpeed: 39 @11:33:12.24 SS.seek 25ml @11:33:57.75 Stroke completed -- waiting for pressure to equalize ... @11:34:02.23 Intake.select :close @11:34:02.39 SSV.dial :exhaust @11:34:03.20 Exhaust.select :open @11:34:03.32 SS.reconfigure SSexhale @11:34:03.37 SS.seek 0ml @11:34:12.18 Exhaust.select :close @11:34:12.30 SSV.dial CBV @11:34:12.32 CBV.dial SSV @11:34:13.14 Allowing 130s for 25.0ml of remaining 1400.0ml @11:34:13.15 Intake.select :open @11:34:15.33 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:34:15.35 SS.reconfigure SSinhale.with maxSpeed: 39 @11:34:15.40 SS.seek 25ml @11:35:00.99 Stroke completed -- waiting for pressure to equalize ... @11:35:06.10 Intake.select :close @11:35:06.26 SSV.dial :exhaust @11:35:07.05 Exhaust.select :open @11:35:07.17 SS.reconfigure SSexhale @11:35:07.21 SS.seek 0ml @11:35:16.16 Exhaust.select :close @11:35:16.28 SSV.dial CBV @11:35:16.31 CBV.dial SSV @11:35:17.11 Allowing 130s for 25.0ml of remaining 1375.0ml @11:35:17.12 Intake.select :open @11:35:19.31 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:35:19.33 SS.reconfigure SSinhale.with maxSpeed: 39 @11:35:19.96 SS.seek 25ml @11:36:05.32 Stroke completed -- waiting for pressure to equalize ... @11:36:09.05 delay 5 seconds @11:36:10.53 Intake.select :close @11:36:10.70 SSV.dial :exhaust @11:36:11.49 Exhaust.select :open @11:36:11.61 SS.reconfigure SSexhale @11:36:11.66 SS.seek 0ml @11:36:14.07 ATV.dial :air2 @11:36:15.07 AS.seek 0.52ml @11:36:20.60 Exhaust.select :close @11:36:20.71 SSV.dial CBV @11:36:20.74 CBV.dial SSV @11:36:21.54 Allowing 130s for 25.0ml of remaining 1350.0ml @11:36:21.55 Intake.select :open @11:36:23.74 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:36:23.76 SS.reconfigure SSinhale.with maxSpeed: 39 @11:36:23.81 SS.seek 25ml @11:37:09.08 Stroke completed -- waiting for pressure to equalize ... @11:37:14.91 Intake.select :close @11:37:15.07 SSV.dial :exhaust @11:37:15.88 Exhaust.select :open @11:37:16.00 SS.reconfigure SSexhale @11:37:16.05 SS.seek 0ml @11:37:25.02 Exhaust.select :close @11:37:25.14 SSV.dial CBV @11:37:25.16 CBV.dial SSV @11:37:25.97 Allowing 130s for 25.0ml of remaining 1325.0ml @11:37:25.98 Intake.select :open @11:37:28.17 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:37:28.19 SS.reconfigure SSinhale.with maxSpeed: 39 @11:37:28.24 SS.seek 25ml @11:37:55.83 delay 5 seconds @11:38:00.86 ATV.dial ASV @11:38:01.80 ASV.dial :wasteR @11:38:02.19 AS.seek 0.3ml @11:38:04.32 Vacuum too high -- waiting up to 40s @11:38:06.17 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:38:06.19 SS.reconfigure SSinhale.with maxSpeed: 35 @11:38:06.24 SS.seek 25ml @11:38:16.76 Stroke completed -- waiting for pressure to equalize ... @11:38:21.20 Intake.select :close @11:38:21.36 SSV.dial :exhaust @11:38:22.19 Exhaust.select :open @11:38:22.31 SS.reconfigure SSexhale @11:38:22.36 SS.seek 0ml @11:38:31.19 Exhaust.select :close @11:38:31.31 SSV.dial CBV @11:38:31.33 CBV.dial SSV @11:38:32.15 Allowing 130s for 25.0ml of remaining 1300.0ml @11:38:32.16 Intake.select :open @11:38:34.35 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:38:34.37 SS.reconfigure SSinhale.with maxSpeed: 35 @11:38:34.42 SS.seek 25ml @11:38:46.72 delay 20 seconds @11:39:06.76 AS.seek 0.07ml @11:39:25.04 Stroke completed -- waiting for pressure to equalize ... @11:39:30.20 Intake.select :close @11:39:30.36 SSV.dial :exhaust @11:39:31.15 Exhaust.select :open @11:39:31.27 SS.reconfigure SSexhale @11:39:31.32 SS.seek 0ml @11:39:40.27 Exhaust.select :close @11:39:40.39 SSV.dial CBV @11:39:40.41 CBV.dial SSV @11:39:41.22 Allowing 130s for 25.0ml of remaining 1275.0ml @11:39:41.23 Intake.select :open @11:39:43.42 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:39:43.44 SS.reconfigure SSinhale.with maxSpeed: 35 @11:39:43.49 SS.seek 25ml @11:39:53.27 delay 20 seconds @11:40:13.30 ATV.dial :waste2 @11:40:14.02 AS.reconfigure ASconfig @11:40:14.07 AS.seek :empty @11:40:18.46 Elution @11:40:18.47 ATV.dial :air1 @11:40:19.54 AS.reconfigure ASslow1 @11:40:19.59 AS.seek 0.049ml @11:40:30.02 delay 3 seconds @11:40:33.05 ATV.dial :ElutionWater @11:40:33.72 AS.seek 0.109ml @11:40:34.02 Stroke completed -- waiting for pressure to equalize ... @11:40:39.15 Intake.select :close @11:40:39.31 SSV.dial :exhaust @11:40:40.12 Exhaust.select :open @11:40:40.24 SS.reconfigure SSexhale @11:40:40.30 SS.seek 0ml @11:40:46.07 delay 3 seconds @11:40:49.09 ATV.dial ASV,via: :air2 @11:40:49.40 Exhaust.select :close @11:40:49.52 SSV.dial CBV @11:40:49.54 CBV.dial SSV @11:40:50.37 Allowing 130s for 25.0ml of remaining 1250.0ml @11:40:50.38 Intake.select :open @11:40:51.08 ASV.dial :SPEelution @11:40:51.65 AFV.select :carrierSolution @11:40:51.81 AS.reconfigure ASnorm @11:40:51.87 AS.seek 0.759ml @11:40:52.56 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:40:52.58 SS.reconfigure SSinhale.with maxSpeed: 35 @11:40:52.63 SS.seek 25ml @11:41:25.54 delay 3 seconds @11:41:28.57 AFV.select :holdingCoil @11:41:28.72 AS.reconfigure ASslow1 @11:41:28.78 AS.seek 0.474ml @11:41:41.67 Vacuum too high -- waiting up to 40s @11:41:43.54 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:41:43.56 SS.reconfigure SSinhale.with maxSpeed: 32 @11:41:43.61 SS.seek 25ml @11:41:45.25 Stroke completed -- waiting for pressure to equalize ... @11:41:49.78 Intake.select :close @11:41:49.94 SSV.dial :exhaust @11:41:50.74 Exhaust.select :open @11:41:50.86 SS.reconfigure SSexhale @11:41:50.91 SS.seek 0ml @11:41:59.91 Exhaust.select :close @11:42:00.03 SSV.dial CBV @11:42:00.05 CBV.dial SSV @11:42:00.88 Allowing 130s for 25.0ml of remaining 1225.0ml @11:42:00.89 Intake.select :open @11:42:03.09 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:42:03.10 SS.reconfigure SSinhale.with maxSpeed: 32 @11:42:03.15 SS.seek 25ml @11:42:26.37 delay 5 seconds @11:42:31.46 AS.reconfigure ASslow2 @11:42:31.51 AS.seek 0.439ml @11:42:57.86 Stroke completed -- waiting for pressure to equalize ... @11:43:03.60 Intake.select :close @11:43:03.76 SSV.dial :exhaust @11:43:04.57 Exhaust.select :open @11:43:04.69 SS.reconfigure SSexhale @11:43:04.74 SS.seek 0ml @11:43:06.93 delay 1:00 @11:43:13.90 Exhaust.select :close @11:43:14.02 SSV.dial CBV @11:43:14.04 CBV.dial SSV @11:43:14.87 Allowing 130s for 25.0ml of remaining 1200.0ml @11:43:14.88 Intake.select :open @11:43:17.07 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:43:17.09 SS.reconfigure SSinhale.with maxSpeed: 32 @11:43:17.14 SS.seek 25ml @11:44:06.96 pushing eluate to holding coil @11:44:07.03 AS.seek 0.279ml @11:44:12.16 Stroke completed -- waiting for pressure to equalize ... @11:44:19.16 Intake.select :close @11:44:19.33 SSV.dial :exhaust @11:44:20.13 Exhaust.select :open @11:44:20.25 SS.reconfigure SSexhale @11:44:20.30 SS.seek 0ml @11:44:29.71 Exhaust.select :close @11:44:29.83 SSV.dial CBV @11:44:29.85 CBV.dial SSV @11:44:30.68 Allowing 130s for 25.0ml of remaining 1175.0ml @11:44:30.69 Intake.select :open @11:44:32.88 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:44:32.90 SS.reconfigure SSinhale.with maxSpeed: 32 @11:44:32.94 SS.seek 25ml @11:45:15.29 Vacuum too high -- waiting up to 40s @11:45:17.38 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:45:17.40 SS.reconfigure SSinhale.with maxSpeed: 29 @11:45:17.45 SS.seek 25ml @11:45:31.59 Stroke completed -- waiting for pressure to equalize ... @11:45:37.35 Intake.select :close @11:45:37.51 SSV.dial :exhaust @11:45:38.32 Exhaust.select :open @11:45:38.44 SS.reconfigure SSexhale @11:45:38.49 SS.seek 0ml @11:45:47.52 Exhaust.select :close @11:45:47.64 SSV.dial CBV @11:45:47.70 CBV.dial SSV @11:45:48.46 Allowing 130s for 25.0ml of remaining 1150.0ml @11:45:48.47 Intake.select :open @11:45:50.66 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:45:50.68 SS.reconfigure SSinhale.with maxSpeed: 29 @11:45:50.73 SS.seek 25ml @11:46:48.04 delay 30 seconds @11:46:51.33 Stroke completed -- waiting for pressure to equalize ... @11:46:58.93 Intake.select :close @11:46:59.09 SSV.dial :exhaust @11:46:59.90 Exhaust.select :open @11:47:00.02 SS.reconfigure SSexhale @11:47:00.07 SS.seek 0ml @11:47:09.18 Exhaust.select :close @11:47:09.30 SSV.dial CBV @11:47:09.32 CBV.dial SSV @11:47:10.15 Allowing 130s for 25.0ml of remaining 1125.0ml @11:47:10.17 Intake.select :open @11:47:12.35 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:47:12.37 SS.reconfigure SSinhale.with maxSpeed: 29 @11:47:12.42 SS.seek 25ml @11:47:18.07 ATV.dial :waste,via: :air3 @11:47:19.70 AS.reconfigure ASconfig @11:47:19.75 AS.seek :empty @11:47:36.15 Parking Bleach in LysateSource line and SPEcolumn @11:47:36.16 ATV.dial :air3 @11:47:36.97 AS.reconfigure ASslow1 @11:47:37.02 AS.seek 0.029ml @11:47:43.37 ATV.dial :ElutionWater @11:47:43.97 AS.seek 0.059ml @11:47:50.29 ATV.dial :air3 @11:47:50.89 AS.seek 0.069ml @11:47:53.15 delay 2 seconds @11:47:55.17 ATV.dial :bleach @11:47:55.64 AS.seek 0.769ml @11:47:59.26 Vacuum too high -- waiting up to 40s @11:48:01.87 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:48:01.89 SS.reconfigure SSinhale.with maxSpeed: 26 @11:48:01.94 SS.seek 25ml @11:48:17.53 Stroke completed -- waiting for pressure to equalize ... @11:48:24.49 Intake.select :close @11:48:24.65 SSV.dial :exhaust @11:48:25.45 Exhaust.select :open @11:48:25.57 SS.reconfigure SSexhale @11:48:25.62 SS.seek 0ml @11:48:34.68 Exhaust.select :close @11:48:34.80 SSV.dial CBV @11:48:34.82 CBV.dial SSV @11:48:35.63 Allowing 130s for 25.0ml of remaining 1100.0ml @11:48:35.64 Intake.select :open @11:48:37.83 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:48:37.85 SS.reconfigure SSinhale.with maxSpeed: 26 @11:48:37.90 SS.seek 25ml @11:49:45.24 Stroke completed -- waiting for pressure to equalize ... @11:49:54.22 Intake.select :close @11:49:54.39 SSV.dial :exhaust @11:49:55.20 Exhaust.select :open @11:49:55.32 SS.reconfigure SSexhale @11:49:55.38 SS.seek 0ml @11:50:04.48 Exhaust.select :close @11:50:04.60 SSV.dial CBV @11:50:04.62 CBV.dial SSV @11:50:05.45 Allowing 130s for 25.0ml of remaining 1075.0ml @11:50:05.46 Intake.select :open @11:50:07.65 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:50:07.67 SS.reconfigure SSinhale.with maxSpeed: 26 @11:50:07.72 SS.seek 25ml @11:50:16.64 delay 3 seconds @11:50:19.67 ATV.dial :air3 @11:50:20.21 delay 3 seconds @11:50:23.30 AS.seek 0.794ml @11:50:28.59 delay 3 seconds @11:50:31.62 ATV.dial :bleach @11:50:32.22 AS.seek 0.779ml @11:50:35.48 delay 3 seconds @11:50:38.51 ATV.dial :AIV @11:50:39.71 AS.reconfigure ASconfig @11:50:39.76 AS.seek 0.015ml @11:50:51.55 Vacuum too high -- waiting up to 40s @11:50:54.18 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:50:54.20 SS.reconfigure SSinhale.with maxSpeed: 23 @11:50:54.25 SS.seek 25ml @11:51:21.40 Stroke completed -- waiting for pressure to equalize ... @11:51:23.96 ATV.dial :waste @11:51:25.39 AS.seek :empty @11:51:26.59 ATV.dial :air3 @11:51:27.23 AS.reconfigure ASslow1 @11:51:27.28 AS.seek 0.009ml @11:51:29.63 delay 2 seconds @11:51:29.72 Intake.select :close @11:51:29.89 SSV.dial :exhaust @11:51:30.70 Exhaust.select :open @11:51:30.82 SS.reconfigure SSexhale @11:51:30.87 SS.seek 0ml @11:51:31.68 ATV.dial :bleach @11:51:32.14 AS.seek 0.579ml @11:51:39.99 Exhaust.select :close @11:51:40.11 SSV.dial CBV @11:51:40.13 CBV.dial SSV @11:51:40.95 Allowing 130s for 25.0ml of remaining 1050.0ml @11:51:40.96 Intake.select :open @11:51:43.15 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:51:43.17 SS.reconfigure SSinhale.with maxSpeed: 23 @11:51:43.21 SS.seek 25ml @11:52:50.83 Vacuum too high -- waiting up to 40s @11:52:53.73 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:52:53.75 SS.reconfigure SSinhale.with maxSpeed: 21 @11:52:53.80 SS.seek 25ml @11:53:03.40 Stroke completed -- waiting for pressure to equalize ... @11:53:12.36 Intake.select :close @11:53:12.52 SSV.dial :exhaust @11:53:13.34 Exhaust.select :open @11:53:13.47 SS.reconfigure SSexhale @11:53:13.52 SS.seek 0ml @11:53:22.60 Exhaust.select :close @11:53:22.72 SSV.dial CBV @11:53:22.74 CBV.dial SSV @11:53:23.57 Allowing 130s for 25.0ml of remaining 1025.0ml @11:53:23.58 Intake.select :open @11:53:25.77 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:53:25.79 SS.reconfigure SSinhale.with maxSpeed: 21 @11:53:25.84 SS.seek 25ml @11:53:27.01 delay 3 seconds @11:53:30.03 ATV.dial :air3 @11:53:30.56 delay 3 seconds @11:53:33.65 AS.seek 0.604ml @11:53:38.94 delay 3 seconds @11:53:41.97 ATV.dial :bleach @11:53:42.58 AS.seek 0.589ml @11:53:45.86 delay 3 seconds @11:53:48.89 ATV.dial :ASV @11:53:49.87 ASV.dial :wasteR @11:53:50.45 AS.seek 0ml @11:54:44.92 Vacuum too high -- waiting up to 40s @11:54:48.34 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:54:48.36 SS.reconfigure SSinhale.with maxSpeed: 19 @11:54:48.41 SS.seek 25ml @11:54:53.78 Stroke completed -- waiting for pressure to equalize ... @11:55:03.33 Intake.select :close @11:55:03.50 SSV.dial :exhaust @11:55:04.32 Exhaust.select :open @11:55:04.44 SS.reconfigure SSexhale @11:55:04.49 SS.seek 0ml @11:55:13.60 Exhaust.select :close @11:55:13.72 SSV.dial CBV @11:55:13.74 CBV.dial SSV @11:55:14.56 Allowing 130s for 25.0ml of remaining 1000.0ml @11:55:14.57 Intake.select :open @11:55:16.75 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:55:16.77 SS.reconfigure SSinhale.with maxSpeed: 19 @11:55:16.82 SS.seek 25ml @11:55:49.84 delay 1:00 @11:56:43.70 Vacuum too high -- waiting up to 40s @11:56:47.09 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:56:47.11 SS.reconfigure SSinhale.with maxSpeed: 17 @11:56:47.16 SS.seek 25ml @11:56:49.87 AFV.select :carrierSolution @11:56:49.97 delay 2 seconds @11:56:52.00 AS.reconfigure ASconfig @11:56:52.05 AS.seek 1ml @11:56:54.07 Stroke completed -- waiting for pressure to equalize ... @11:57:03.68 Intake.select :close @11:57:03.84 SSV.dial :exhaust @11:57:04.64 Exhaust.select :open @11:57:04.76 SS.reconfigure SSexhale @11:57:04.81 SS.seek 0ml @11:57:13.75 Exhaust.select :close @11:57:13.87 SSV.dial CBV @11:57:13.90 CBV.dial SSV @11:57:14.71 Allowing 130s for 25.0ml of remaining 975.0ml @11:57:14.72 Intake.select :open @11:57:16.91 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:57:16.93 SS.reconfigure SSinhale.with maxSpeed: 17 @11:57:16.97 SS.seek 25ml @11:57:49.84 delay 1 second @11:57:50.86 AFV.select :holdingCoil @11:57:50.96 delay 1 second @11:57:51.99 ATV.dial ASV @11:57:52.00 ASV.dial :wasteR @11:57:52.02 AS.reconfigure ASslow1 @11:57:52.06 AS.seek 0ml @11:58:57.27 Vacuum too high -- waiting up to 40s @11:59:00.94 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:59:00.96 SS.reconfigure SSinhale.with maxSpeed: 15 @11:59:01.00 SS.seek 25ml @11:59:04.88 Stroke completed -- waiting for pressure to equalize ... @11:59:15.14 Intake.select :close @11:59:15.30 SSV.dial :exhaust @11:59:16.09 Exhaust.select :open @11:59:16.21 SS.reconfigure SSexhale @11:59:16.26 SS.seek 0ml @11:59:25.23 Exhaust.select :close @11:59:25.35 SSV.dial CBV @11:59:25.37 CBV.dial SSV @11:59:26.20 Allowing 130s for 25.0ml of remaining 950.0ml @11:59:26.21 Intake.select :open @11:59:28.40 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:59:28.42 SS.reconfigure SSinhale.with maxSpeed: 15 @11:59:28.47 SS.seek 25ml @12:01:13.44 delay 20 seconds @12:01:25.51 Stroke completed -- waiting for pressure to equalize ... @12:01:33.46 ATV.dial :air3 @12:01:34.90 AS.seek 0.59ml @12:01:36.23 SS.reconfigure SSconfig @12:01:36.28 SS.hold @12:01:36.42 Intake.select :close @12:01:36.58 ShallowSampler::Clogged in WCR -- progress too slow after 575.0ml @12:01:36.60 SSV.dial :exhaust @12:01:37.40 Exhaust.select :open @12:01:37.52 SS.reconfigure SSexhale @12:01:37.57 SS.seek 0ml @12:01:46.53 Exhaust.select :close @12:01:46.64 Sampled 575.0ml @12:01:46.66 Drying puck before fixing for 4:00 @12:01:46.67 CBV.dial :waste2b @12:01:47.46 delay 1:00 @12:02:47.49 CSV.dial :air @12:02:47.94 CS.reconfigure CSfast @12:02:48.00 CS.seek 10ml @12:03:11.26 Pumping wcr puck from tube 2 up to 55psi... @12:03:11.28 CBV.dial SSV @12:03:12.63 CBV.dial between CSV and waste2b @12:03:13.66 CSV.dial between air and CTV @12:03:14.02 CS.seek 7.193ml @12:03:21.36 CTV.dial CSV,avoiding: :air @12:03:21.38 CSV.dial CTV @12:03:22.40 CS.reconfigure CSpressurize @12:03:22.45 CS.seek 0ml @12:03:33.83 delay 3 seconds @12:03:36.86 ATV.dial :ASV @12:03:38.21 ASV.dial :wasteR @12:03:38.30 AS.seek 0.09ml @12:03:40.03 CBV.dial :waste2b @12:03:40.38 CS.reconfigure CSconfig @12:03:40.44 CS.seek :empty @12:03:42.70 delay 1:00 @12:04:42.73 CTV.dial :air,avoiding: :intake @12:04:44.10 Fix #1 of 2 (with rnal) for 2:00 @12:04:44.12 CSV.dial CSR @12:04:44.65 Pulling 2ml of CSR.rnal @12:04:44.67 CSR.select :rnal @12:04:44.79 CS.seek 2ml @12:04:50.98 delay 5 seconds @12:04:56.01 CSR.select :air @12:04:56.14 CS.reconfigure CSfast @12:04:56.19 CS.seek 10ml @12:05:14.98 Pumping wcr puck from tube 2 up to 55psi... @12:05:15.00 CBV.dial SSV @12:05:16.35 CBV.dial between CSV and waste2b @12:05:17.38 CSV.dial between air and CTV @12:05:18.02 CS.seek 7.158ml @12:05:19.07 delay 30 seconds @12:05:25.36 CTV.dial CSV,avoiding: :air @12:05:25.38 CSV.dial CTV @12:05:26.82 CS.reconfigure CSpressurize @12:05:26.88 CS.seek 0ml @12:05:45.16 delay 2:00 @12:05:49.10 ATV.dial :extSrc @12:05:49.85 delay 3 seconds @12:05:52.88 ATV.dial :ElutionWater @12:05:53.95 AS.seek 0.12ml @12:06:00.27 ATV.dial :air3 @12:06:00.87 AS.seek 0.13ml @12:06:03.16 delay 2 seconds @12:06:05.18 ATV.dial :bleach @12:06:05.62 AS.seek 0.23ml @12:06:25.97 delay 3 seconds @12:06:29.00 ATV.dial :air3 @12:06:29.54 delay 3 seconds @12:06:32.63 AS.seek 0.255ml @12:06:37.91 delay 3 seconds @12:06:40.94 ATV.dial :bleach @12:06:41.54 AS.seek 0.24ml @12:06:44.82 delay 3 seconds @12:06:47.84 ATV.dial :ASV @12:06:48.83 ASV.dial :SPEdelivery @12:06:49.21 AFV.select :carrierSolution @12:06:49.32 delay 2 seconds @12:06:51.35 AS.reconfigure ASconfig @12:06:51.40 AS.seek 0.5ml @12:07:06.63 delay 1 second @12:07:07.65 AFV.select :holdingCoil @12:07:07.80 AS.reconfigure ASslow1 @12:07:07.85 AS.seek 0.17ml @12:07:45.19 CBV.dial :waste2b @12:07:45.54 CS.reconfigure CSconfig @12:07:45.59 CS.seek :empty @12:07:46.95 delay 1:00 @12:08:14.52 SPE.hold 2:46:40,95C @12:08:14.58 delay 3:00 @12:08:46.98 CTV.dial :air,avoiding: :intake @12:08:48.40 Fix #2 of 2 (with rnal) for 2:00 @12:08:48.42 CSV.dial CSR @12:08:48.95 Pulling 2ml of CSR.rnal @12:08:48.97 CSR.select :rnal @12:08:49.09 CS.seek 2ml @12:08:55.83 delay 5 seconds @12:09:00.86 CSR.select :air @12:09:00.99 CS.reconfigure CSfast @12:09:01.04 CS.seek 10ml @12:09:20.64 Pumping wcr puck from tube 2 up to 55psi... @12:09:20.66 CBV.dial SSV @12:09:22.04 CBV.dial between CSV and waste2b @12:09:23.10 CSV.dial between air and CTV @12:09:23.76 CS.seek 7.12ml @12:09:31.53 CTV.dial CSV,avoiding: :air @12:09:31.57 CSV.dial CTV @12:09:33.04 CS.reconfigure CSpressurize @12:09:33.09 CS.seek 0ml @12:09:51.01 delay 2:00 @12:11:14.63 AFV.select :carrierSolution @12:11:14.73 delay 2 seconds @12:11:16.76 AS.reconfigure ASconfig @12:11:16.81 AS.seek 1ml @12:11:51.04 CBV.dial :waste2b @12:11:51.39 CS.reconfigure CSconfig @12:11:51.44 CS.seek :empty @12:11:53.21 delay 1:00 @12:12:04.81 delay 1 second @12:12:05.83 AFV.select :holdingCoil @12:12:05.93 delay 1 second @12:12:06.96 ASV.dial :wasteD @12:12:07.94 AS.seek 0ml @12:12:53.24 CTV.dial :air,avoiding: :intake @12:12:54.71 Storing wcr puck in Collection Clamp to tube 1 @12:12:54.90 SC.reconfigure SCtight @12:12:54.96 SC.seek 1 @12:12:57.26 SC.reconfigure SCloose @12:12:57.49 Elbow.reconfigure TightElbow @12:12:57.54 Elbow.seek 1 @12:12:59.48 Forearm.reconfigure TightForearm @12:12:59.53 Forearm.seek 1 + 700 counts @12:13:00.58 SE.reconfigure SEpick @12:13:00.66 SE.seek beamBreak @12:13:02.51 SE.configure SEconfig @12:13:02.69 Elbow.reconfigure LimpElbow @12:13:02.74 Forearm.reconfigure LooseForearm @12:13:02.79 Forearm.seek 1 @12:13:03.06 Elbow.hold @12:13:03.14 Elbow.reconfigure TightElbow @12:13:03.25 SE.seek 13.1 Pucks below Top Plate @12:13:03.74 Forearm.force -20 @12:13:03.79 Forearm.seek 1 + 347 counts @12:13:04.12 Forearm.reconfigure TightForearm @12:13:04.17 Forearm.seek 1 + 700 counts @12:13:04.41 SE.seek 14.1 Pucks below Top Plate @12:13:05.09 Forearm.reconfigure LooseForearm @12:13:05.17 Forearm.seek :retract @12:13:05.88 Priming Eluate @12:13:05.89 AFV.select :carrierSolution @12:13:06.00 delay 2 seconds @12:13:06.76 Elbow.seek CC @12:13:08.03 AS.seek 0.5ml @12:13:08.46 Elbow.reconfigure LooseElbow @12:13:08.58 Forearm.seek CC @12:13:09.64 Hand.close @12:13:10.24 CC.seek :open @12:13:14.78 Forearm.seek :retract @12:13:15.84 Elbow.reconfigure TightElbow @12:13:15.89 Elbow.seek 1 @12:13:17.86 Elbow.reconfigure LimpElbow @12:13:17.92 Forearm.seek 1 @12:13:18.97 Elbow.hold @12:13:19.05 Elbow.reconfigure TightElbow @12:13:19.10 Hand.open @12:13:19.87 Forearm.force -20 @12:13:19.92 Forearm.seek 1 + 347 counts @12:13:20.27 Forearm.reconfigure TightForearm @12:13:20.32 Forearm.seek 1 + 700 counts @12:13:20.70 SE.seek :down @12:13:21.84 Forearm.reconfigure LooseForearm @12:13:21.89 Forearm.seek :retract @12:13:22.20 SE.coast @12:13:22.83 SC.coast @12:13:22.87 Starting finalPurge @12:13:22.89 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:13:22.97 Hand.open @12:13:23.37 Elbow.seek FlushPuck::Garage @12:13:25.44 Elbow.reconfigure LooseElbow @12:13:25.55 Forearm.seek :garage @12:13:26.56 Hand.close @12:13:27.36 Forearm.seek :retract @12:13:28.36 Elbow.reconfigure TightElbow @12:13:28.41 Elbow.seek CC @12:13:31.34 Elbow.reconfigure LooseElbow @12:13:31.44 Forearm.reconfigure TightForearm @12:13:31.49 Forearm.seek CC @12:13:32.83 CC.seek :closed @12:13:37.08 delay 1 second @12:13:37.15
Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 1.95 @12:13:37.24 Hand.open @12:13:37.93 Forearm.reconfigure LooseForearm @12:13:37.99 Forearm.seek :retract @12:13:38.15 AFV.select :holdingCoil @12:13:38.25 ATV.dial ASV @12:13:38.26 ASV.dial :SPErecovery @12:13:38.84 Swish #1 of 2 @12:13:38.92 AS.reconfigure ASslow0 @12:13:38.98 AS.seek 0.6ml @12:13:39.05
Flushing the Collection Valve::Manifold @12:13:39.19 CSV.dial CSR @12:13:39.72 Pulling 5ml of CSR.flush CSR.select :flush @12:13:39.85 CS.seek 5ml @12:13:49.46 AS.seek 0.5ml @12:13:53.68
delay 5 seconds @12:13:58.71 CSR.select :air @12:13:58.88 CS.reconfigure CSfast @12:13:58.93 CS.seek 7.5ml @12:13:59.80 Swish #2 of 2 @12:13:59.89 AS.seek 0.6ml @12:14:05.71
CSV.dial :waste @12:14:07.07 CS.reconfigure CSconfig @12:14:07.12 CS.seek 0ml @12:14:10.39 AS.seek 0.5ml @12:14:20.78 delay 3 seconds @12:14:23.86 AS.reconfigure ASslow1 @12:14:23.92 AS.seek 0.684ml @12:14:27.46
Flushing lower manifold's waste1 port @12:14:27.60 CSV.dial :flush @12:14:28.08 CS.reconfigure CSfast @12:14:28.12 CS.seek 5ml @12:14:40.52 delay 5 seconds @12:14:45.55 CSV.dial :air @12:14:46.09 CS.seek 7ml @12:14:51.74 CSV.dial CTV @12:14:51.78
CTV.dial CSV @12:14:53.22 CBV.dial :waste1 @12:14:54.05 CS.seek 0ml @12:15:01.21 delay 3 seconds @12:15:04.24 Eluate primed @12:15:04.25 ATV.dial :waste2 @12:15:04.84 AS.reconfigure ASnorm @12:15:04.89 AS.seek 0ml @12:15:10.93
Flushing lower manifold's waste2b port @12:15:11.07 CSV.dial :flush @12:15:11.86 CS.seek 5ml @12:15:24.24 delay 5 seconds @12:15:29.27 CSV.dial :air @12:15:29.83 CS.seek 7ml @12:15:35.52 CSV.dial CTV @12:15:35.55
CTV.dial CSV @12:15:36.02 CBV.dial :waste2b @12:15:36.83 CS.seek 0ml @12:15:39.67 SPE.coast @12:15:39.76 ASV.dial :SPErecovery @12:15:39.78 Setting up PCR for OM -- writing /var/log/esp/OM20may1712h525ml.pcr @12:15:39.80 Building PCR Slug from ASV with MM3 mix, PP5 primer @12:15:39.81 Priming with carrier @12:15:39.82 ATV.dial ARV @12:15:40.77 ARV.dial :carrier @12:15:41.13 AS.seek 0.5ml @12:15:54.09
Flushing upper manifold @12:15:54.23 CSV.dial :flush @12:15:55.00 CS.seek 5ml @12:16:06.71 AFV.select :carrierSolution @12:16:06.82 delay 2 seconds @12:16:07.48
delay 5 seconds @12:16:09.48 AS.reconfigure ASconfig @12:16:09.54 AS.seek 1ml @12:16:12.51
CSV.dial :air @12:16:13.06 CS.seek 7ml @12:16:18.55 CSV.dial CBV @12:16:18.58
CBV.dial CSV @12:16:19.73 CTV.dial :air @12:16:21.11 CS.seek 0ml @12:16:37.71 delay 7 seconds @12:16:38.57 delay 1 second @12:16:39.59 AFV.select :holdingCoil @12:16:39.69 delay 1 second @12:16:40.72 ATV.dial :waste @12:16:42.15 AS.reconfigure ASnorm @12:16:42.21 AS.seek :empty @12:16:44.74
CS.seek 3ml @12:16:52.40 CSV.dial :waste @12:16:52.89 CS.seek 0ml @12:17:00.72 CSV.dial :flush @12:17:01.19 CS.seek 3ml @12:17:08.96 delay 5 seconds @12:17:13.99 CSV.dial :air @12:17:14.53 CS.seek 5ml @12:17:20.02 CSV.dial :air @12:17:20.04 CS.seek 0ml @12:17:32.18 delay 7 seconds @12:17:32.95 AFV.select :carrierSolution @12:17:33.06 delay 2 seconds @12:17:35.09 AS.reconfigure ASconfig @12:17:35.14 AS.seek 0.5ml @12:17:39.21
CS.seek 3ml @12:17:46.93 CSV.dial :waste @12:17:47.69 CS.seek 0ml @12:17:55.40 Disinfecting external Intake with mfbkill @12:17:55.54 CSV.dial CSR @12:17:56.94 Pulling 8ml of CSR.mfbkill CSR.select :mfbkill @12:17:57.07 CS.seek 8ml @12:18:04.25 delay 1 second @12:18:05.27 AFV.select :holdingCoil @12:18:05.37 Building Slug @12:18:05.38 ATV.dial :ARV @12:18:06.93 delay 2 seconds @12:18:08.96 ARV.dial :air1 @12:18:09.39 delay 2 seconds @12:18:11.48 AS.reconfigure ASslow2 @12:18:11.53 AS.seek 0.506ml @12:18:15.74
delay 5 seconds @12:18:17.79 delay 2 seconds @12:18:19.81 ARV.dial :MM3 @12:18:20.76
CSR.select :air @12:18:20.82 delay 2 seconds @12:18:20.95
CS.seek 10ml @12:18:22.85 Adding MM3 @12:18:22.93 AS.seek 0.524ml @12:18:26.43 CSV.dial CBV @12:18:26.46
CBV.dial CSV @12:18:27.51 CTV.dial :intake @12:18:28.29 Intake.select :open @12:18:28.41 CS.seek 2ml @12:18:41.45 delay 25 seconds @12:18:48.28
Intake.select :close @12:18:48.39 CTV.dial :air @12:18:49.18 CS.reconfigure CSconfig @12:18:49.23 CS.seek 0ml @12:18:55.53 Flushing lower manifold's waste1 port @12:18:55.54 CSV.dial CSR @12:18:56.65 Pulling 3.999ml of CSR.mfbkill @12:18:56.66 CSR.select :mfbkill @12:18:56.79 CS.seek 4ml @12:19:06.48 ARV.dial :PP5 @12:19:06.87 Adding PP5 @12:19:06.89 delay 2 seconds @12:19:08.03
delay 5 seconds @12:19:08.97 AS.seek 0.53ml @12:19:13.05
CSR.select :air @12:19:13.17 CS.reconfigure CSfast @12:19:13.21 CS.seek 10ml @12:19:15.17 delay 10 seconds @12:19:25.20 ARV.dial :air3 @12:19:26.17 adding ARV air @12:19:26.18 delay 2 seconds @12:19:27.59 CSV.dial CTV @12:19:27.62
CTV.dial CSV @12:19:28.27 AS.seek 0.536ml @12:19:29.07
CBV.dial :waste1 @12:19:29.57 CS.seek 0ml @12:19:34.73 delay 5 seconds @12:19:39.76 ARV.dial :carrier @12:19:40.97 delay 7 seconds @12:19:48.06 AS.seek 0.594ml @12:19:52.87
Flushing lower manifold's waste2b port @12:19:52.88 CSV.dial CSR @12:19:53.44 Pulling 4ml of CSR.mfbkill @12:19:53.45 CSR.select :mfbkill @12:19:53.57 CS.reconfigure CSconfig @12:19:53.62 CS.seek 4ml @12:20:04.78 delay 5 seconds @12:20:09.81 CSR.select :air @12:20:09.94 CS.reconfigure CSfast @12:20:09.98 CS.seek 10ml @12:20:24.23 CSV.dial CTV @12:20:24.25
CTV.dial CSV @12:20:24.75 CBV.dial :waste2b @12:20:25.55 CS.seek 0ml @12:20:46.56 delay 5 seconds @12:20:48.72
Killing upper manifold @12:20:48.73 CSV.dial CSR @12:20:49.29 Pulling 4ml of CSR.mfbkill @12:20:49.30 CSR.select :mfbkill @12:20:49.42 CS.reconfigure CSconfig @12:20:49.47 CS.seek 4ml @12:20:51.59 ATV.dial :ASV @12:20:51.98 delay 2 seconds @12:20:54.07 AS.seek 0.6ml @12:21:00.23 delay 4 seconds @12:21:00.65
delay 5 seconds @12:21:04.26 ATV.dial ARV @12:21:04.80 delay 2 seconds @12:21:05.67
CSR.select :air @12:21:05.79 CS.reconfigure CSfast @12:21:05.83 CS.seek 10ml @12:21:06.83 ARV.dial :carrier @12:21:06.85 delay 2 seconds @12:21:08.92 AS.seek 0.65ml @12:21:19.97 CSV.dial CBV @12:21:20.00
CBV.dial CSV @12:21:21.09 CTV.dial :air @12:21:22.45 CS.seek 0ml @12:21:45.44 delay 7 seconds @12:21:52.48 CS.seek 3ml @12:21:59.36 delay 4 seconds @12:22:00.25
CSV.dial :waste @12:22:00.71 CS.seek 0ml @12:22:03.39 ATV.dial PCR @12:22:03.98 ASV.dial :waste1 @12:22:04.73 ARV.dial :air1 @12:22:05.11 Positioning Slug by 0.324ml @12:22:05.20 AS.seek 0.326ml @12:22:08.44
Flushing upper manifold @12:22:08.58 CSV.dial :flush @12:22:09.06 CS.seek 5ml @12:22:21.03 delay 5 seconds @12:22:26.06 CSV.dial :air @12:22:26.60 CS.seek 7ml @12:22:32.09 CSV.dial CBV @12:22:32.12
CBV.dial CSV @12:22:33.19 CTV.dial :air @12:22:33.21 CS.seek 0ml @12:22:49.63 delay 7 seconds @12:22:56.67 CS.seek 3ml @12:23:04.31 CSV.dial :waste @12:23:04.79 CS.seek 0ml @12:23:13.15 CSV.dial :flush @12:23:13.64 CS.seek 3ml @12:23:21.47 delay 5 seconds @12:23:26.50 CSV.dial :air @12:23:27.05 CS.seek 5ml @12:23:32.53 CSV.dial :air @12:23:32.55 CS.seek 0ml @12:23:44.53 delay 7 seconds @12:23:51.57 CS.seek 3ml @12:23:59.38 CSV.dial :waste @12:24:00.15 CS.seek 0ml @12:24:07.81 Flushing lower manifold's waste1 port @12:24:07.95 CSV.dial :flush @12:24:08.43 CS.seek 5ml @12:24:20.47 delay 5 seconds @12:24:25.50 CSV.dial :air @12:24:26.15 CS.seek 7ml @12:24:31.69 CSV.dial CTV @12:24:31.72
CTV.dial CSV @12:24:33.16 CBV.dial :waste1 @12:24:33.67 CS.seek 0ml @12:24:50.57 Flushing lower manifold's waste2b port @12:24:50.71 CSV.dial :flush @12:24:51.48 CS.seek 5ml @12:25:03.57 delay 5 seconds @12:25:08.60 CSV.dial :air @12:25:09.15 CS.seek 7ml @12:25:14.57 CSV.dial CTV @12:25:14.60
CTV.dial CSV @12:25:15.07 CBV.dial :waste2b @12:25:15.87 CS.seek 0ml @12:25:32.22 Disinfecting external Intake with kill @12:25:32.36 CSV.dial CSR @12:25:32.89 Pulling 8ml of CSR.kill @12:25:32.90 CSR.select :kill @12:25:33.03 CS.seek 8ml @12:25:51.57 delay 5 seconds @12:25:56.60 CSR.select :air @12:25:56.73 CS.seek 10ml @12:26:02.20 CSV.dial CBV @12:26:02.24
CBV.dial CSV @12:26:03.32 CTV.dial :intake @12:26:04.08 Intake.select :open @12:26:04.20 CS.seek 2ml @12:26:23.82 Intake.select :close @12:26:23.93 CTV.dial :air @12:26:24.71 CS.reconfigure CSconfig @12:26:24.76 CS.seek 0ml @12:26:30.82 CSV.dial CSR @12:26:32.05 Pulling 5ml of CSR.flush @12:26:32.06 CSR.select :flush @12:26:32.19 CS.seek 5ml @12:26:45.97 delay 5 seconds @12:26:51.00 CSR.select :air @12:26:51.13 CS.seek 10ml @12:27:04.82 CSV.dial CBV @12:27:05.86 CS.seek 0ml @12:27:31.21 delay 5 seconds @12:27:32.54
Garaging FlushPuck in Collection Clamp @12:27:32.62 Elbow.reconfigure TightElbow @12:27:32.67 Elbow.seek CC @12:27:32.94 Elbow.reconfigure LooseElbow @12:27:33.04 Forearm.seek CC @12:27:34.04 Hand.close @12:27:34.64 CC.seek :open @12:27:36.23 ATV.dial ARV @12:27:36.99 ASV.dial :PCRlock @12:27:37.99 delay 5 seconds @12:27:39.05
Forearm.seek :retract @12:27:40.09 Elbow.reconfigure TightElbow @12:27:40.14 Elbow.seek :garage @12:27:43.02 Running OM Profile60C350 thermocycling @12:27:43.04 PCR.program Profile60C350 @12:27:43.15 Profile60C350 should complete in < 2:02:45 @12:27:43.16 PCR.run 2:38:26.25 @12:27:43.38
Elbow.reconfigure LooseElbow @12:27:43.48 Forearm.reconfigure TightForearm @12:27:43.53 Forearm.seek :garage @12:27:44.68 Hand.open @12:27:45.33 Forearm.reconfigure LooseForearm @12:27:45.38 Forearm.seek :retract @12:27:46.40 Starting initialPurge @12:27:46.41 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:27:46.49 Hand.open @12:27:46.83 Elbow.reconfigure TightElbow @12:27:46.88 Elbow.seek FlushPuck::Garage @12:27:47.14 Elbow.reconfigure LooseElbow @12:27:47.24 Forearm.seek :garage @12:27:48.27 Hand.close @12:27:49.02 Forearm.seek :retract @12:27:50.06 Elbow.reconfigure TightElbow @12:27:50.11 Elbow.seek CC @12:27:53.01 Elbow.reconfigure LooseElbow @12:27:53.12 Forearm.reconfigure TightForearm @12:27:53.17 Forearm.seek CC @12:27:54.54 CC.seek :closed @12:27:58.81 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.92 @12:27:58.90 Hand.open @12:27:59.58 Forearm.reconfigure LooseForearm @12:27:59.63 Forearm.seek :retract @12:28:00.65 Flushing the Collection Valve::Manifold @12:28:00.79 CSV.dial CSR @12:28:01.91 Pulling 5ml of CSR.flush @12:28:01.92 CSR.select :flush @12:28:02.05 CS.seek 5ml @12:28:15.91 delay 5 seconds @12:28:20.94 CSR.select :air @12:28:21.10 CS.reconfigure CSfast @12:28:21.15 CS.seek 7.501ml @12:28:27.73 CSV.dial :waste @12:28:29.07 CS.reconfigure CSconfig @12:28:29.12 CS.seek 0ml @12:28:49.32 Purging external Intake @12:28:49.45 CSV.dial :flush @12:28:49.93 CS.reconfigure CSfast @12:28:49.98 CS.seek 10ml @12:29:13.07 delay 5 seconds @12:29:18.09 CSV.dial CBV @12:29:18.12
CBV.dial CSV @12:29:18.91 CTV.dial :intake @12:29:19.70 Intake.select :open @12:29:19.82 CS.seek 0ml @12:29:45.26 Intake.select :close @12:29:45.37 CTV.dial :air @12:29:46.17 Flushing upper manifold @12:29:46.31 CSV.dial :flush @12:29:47.08 CS.seek 5ml @12:29:59.23 delay 5 seconds @12:30:04.25 CSV.dial :air @12:30:04.86 CS.seek 7ml @12:30:10.32 CSV.dial CBV @12:30:10.34
CBV.dial CSV @12:30:11.42 CTV.dial :air @12:30:11.44 CS.seek 0ml @12:30:27.92 delay 7 seconds @12:30:34.95 CS.seek 3ml @12:30:42.60 CSV.dial :waste @12:30:43.08 CS.seek 0ml @12:30:50.94 CSV.dial :flush @12:30:51.43 CS.seek 3ml @12:30:59.07 delay 5 seconds @12:31:04.09 CSV.dial :air @12:31:04.63 CS.seek 5ml @12:31:10.17 CSV.dial :air @12:31:10.19 CS.seek 0ml @12:31:22.26 delay 7 seconds @12:31:29.29 CS.seek 3ml @12:31:36.98 CSV.dial :waste @12:31:37.74 CS.seek 0ml @12:31:45.45 Garaging FlushPuck in Collection Clamp @12:31:45.53 Elbow.reconfigure TightElbow @12:31:45.58 Elbow.seek CC @12:31:45.84 Elbow.reconfigure LooseElbow @12:31:45.95 Forearm.seek CC @12:31:46.97 Hand.close @12:31:47.65 CC.seek :open @12:31:52.02 Forearm.seek :retract @12:31:53.03 Elbow.reconfigure TightElbow @12:31:53.08 Elbow.seek :garage @12:31:56.28 Elbow.reconfigure LooseElbow @12:31:56.39 Forearm.reconfigure TightForearm @12:31:56.43 Forearm.seek :garage @12:31:57.61 Hand.open @12:31:58.34 Forearm.reconfigure LooseForearm @12:31:58.39 Forearm.seek :retract @12:31:59.41 Completed [stdphase] begun 10:01:11 @12:31:59.42 Preparing to sleep until Mon May 18 10:01:11 +0200 2020 @12:31:59.48 deferred until MFBPCR finish @12:31:59.50
Waiting for MFBPCR til 10:01CEST18-May-20 @12:31:59.52 Continuing at 10:01CEST18-May-20 @12:31:59.58 GoodNews.email "Then, in tube 2: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:124\n{1 => 15, 2 => 7}\nCan@12:31:59, 8.8C, 43% humidity, 14.3psia, 13.824V, 0.727A, 0.727A avg, 10.05W",Subject: "Waiting until 10:01CEST18-May-20" @14:03:43.01 PCR.off @14:03:44.29 PCR for OM completed -- closed /var/log/esp/OM20may1712h525ml.pcr @14:03:44.37 Cleaning PCR @14:03:44.38 AFV.select :carrierSolution @14:03:44.49 delay 2 seconds @14:03:46.52 AS.reconfigure ASconfig @14:03:46.57 AS.seek 0.5ml @14:03:56.86 delay 1 second @14:03:57.88 AFV.select :holdingCoil @14:03:57.98 ASV.dial :waste1 @14:03:58.36 ATV.dial :air3 @14:03:59.59 AS.reconfigure ASslow1 @14:03:59.64 AS.seek 0.51ml @14:04:01.92 delay 2 seconds @14:04:03.94 ATV.dial :bleach @14:04:04.54 AS.seek 0.81ml @14:05:05.13 delay 3 seconds @14:05:08.16 ATV.dial :air3 @14:05:08.69 delay 3 seconds @14:05:11.78 AS.seek 0.835ml @14:05:17.08 delay 3 seconds @14:05:20.10 ATV.dial :bleach @14:05:20.70 AS.seek 0.82ml @14:05:23.99 delay 3 seconds @14:05:27.02 ARV.dial :waste @14:05:27.60 delay 3 seconds @14:05:30.63 ATV.dial :ARV @14:05:31.59 AS.reconfigure ASconfig @14:05:31.63 AS.seek 0.47ml @14:05:52.05 AFV.select :carrierSolution @14:05:52.16 delay 2 seconds @14:05:54.20 AS.seek 1ml @14:06:24.98 delay 1 second @14:06:26.00 AFV.select :holdingCoil @14:06:26.10 delay 1 second @14:06:27.13 AS.reconfigure ASfast @14:06:27.18 AS.seek 0ml @14:06:54.52 AFV.select :carrierSolution @14:06:54.63 delay 2 seconds @14:06:56.66 AS.reconfigure ASconfig @14:06:56.71 AS.seek 1ml @14:07:54.51 delay 1 second @14:07:55.53 AFV.select :holdingCoil @14:07:55.63 delay 1 second @14:07:56.66 AS.reconfigure ASfast @14:07:56.71 AS.seek 0ml @14:08:24.06 AFV.select :carrierSolution @14:08:24.17 delay 2 seconds @14:08:26.20 AS.reconfigure ASconfig @14:08:26.25 AS.seek 1ml @14:09:24.01 delay 1 second @14:09:25.03 AFV.select :holdingCoil @14:09:25.13 delay 1 second @14:09:26.16 AS.reconfigure ASfast @14:09:26.21 AS.seek 0ml @14:09:54.17 ARV.dial between carrier and air1 @14:09:54.65 ATV.dial :air3 @14:09:55.88 AS.reconfigure ASslow1 @14:09:55.93 AS.seek 0.01ml @14:09:58.23 delay 2 seconds @14:10:00.25 ATV.dial :bleach @14:10:00.86 AS.seek 0.81ml @14:12:41.98 delay 3 seconds @14:12:45.01 ATV.dial :air3 @14:12:45.54 delay 3 seconds @14:12:48.63 AS.seek 0.835ml @14:12:53.94 delay 3 seconds @14:12:56.97 ATV.dial :bleach @14:12:57.58 AS.seek 0.82ml @14:13:00.87 delay 3 seconds @14:13:03.90 ATV.dial PCR @14:13:04.28 AS.reconfigure ASconfig @14:13:04.33 AS.seek :empty @14:13:51.79 PCR.program :bleachProfile @14:13:51.85 PCR program should complete in < 2:45 @14:13:51.86 PCR.run 10:00 @14:17:05.80 PCR.off @14:17:05.84 AFV.select :carrierSolution @14:17:05.95 delay 2 seconds @14:17:07.99 AS.seek 1ml @14:18:05.80 delay 1 second @14:18:06.82 AFV.select :holdingCoil @14:18:06.92 delay 1 second @14:18:07.95 AS.reconfigure ASfast @14:18:08.00 AS.seek 0ml @14:18:35.31 delay 3 seconds @14:18:38.34 AFV.select :carrierSolution @14:18:38.44 delay 2 seconds @14:18:40.47 AS.reconfigure ASconfig @14:18:40.52 AS.seek 1ml @14:19:38.30 delay 1 second @14:19:39.32 AFV.select :holdingCoil @14:19:39.42 delay 1 second @14:19:40.45 AS.reconfigure ASfast @14:19:40.50 AS.seek 0ml @14:20:07.83 delay 3 seconds @14:20:10.86 AFV.select :carrierSolution @14:20:10.96 delay 2 seconds @14:20:12.99 AS.reconfigure ASconfig @14:20:13.05 AS.seek 1ml @14:21:10.81 delay 1 second @14:21:11.83 AFV.select :holdingCoil @14:21:11.93 delay 1 second @14:21:12.96 AS.reconfigure ASfast @14:21:13.01 AS.seek 0ml @14:21:40.35 delay 3 seconds @14:21:43.38 AFV.select :carrierSolution @14:21:43.48 delay 2 seconds @14:21:45.51 AS.reconfigure ASconfig @14:21:45.56 AS.seek 1ml @14:22:43.33 delay 1 second @14:22:44.35 AFV.select :holdingCoil @14:22:44.45 delay 1 second @14:22:45.48 AS.reconfigure ASfast @14:22:45.53 AS.seek 0ml @14:23:12.85 delay 3 seconds @14:23:15.88 AFV.select :carrierSolution @14:23:15.98 delay 2 seconds @14:23:18.02 AS.reconfigure ASconfig @14:23:18.07 AS.seek 1ml @14:24:15.82 delay 1 second @14:24:16.84 AFV.select :holdingCoil @14:24:16.94 delay 1 second @14:24:17.97 AS.reconfigure ASnorm @14:24:18.02 AS.seek 0ml @14:25:08.78 Setting up PCR for ML -- writing /var/log/esp/ML20may1714h525ml.pcr @14:25:08.80 Building PCR Slug from ASV with MM2 mix, PP4 primer @14:25:08.81 Priming with carrier @14:25:08.82 ATV.dial ARV @14:25:09.41 ARV.dial :carrier @14:25:09.75 AS.seek 0.5ml @14:25:35.29 AFV.select :carrierSolution @14:25:35.40 delay 2 seconds @14:25:37.43 AS.reconfigure ASconfig @14:25:37.48 AS.seek 1ml @14:26:06.52 delay 1 second @14:26:07.54 AFV.select :holdingCoil @14:26:07.64 delay 1 second @14:26:08.67 ATV.dial :waste @14:26:10.24 AS.reconfigure ASnorm @14:26:10.29 AS.seek :empty @14:27:01.02 AFV.select :carrierSolution @14:27:01.13 delay 2 seconds @14:27:03.16 AS.reconfigure ASconfig @14:27:03.21 AS.seek 0.5ml @14:27:32.27 delay 1 second @14:27:33.29 AFV.select :holdingCoil @14:27:33.39 Building Slug @14:27:33.40 ATV.dial :ARV @14:27:34.98 delay 2 seconds @14:27:37.01 ARV.dial :air1 @14:27:37.41 delay 2 seconds @14:27:39.50 AS.reconfigure ASslow2 @14:27:39.56 AS.seek 0.506ml @14:27:45.79 delay 2 seconds @14:27:47.82 ARV.dial :MM2 @14:27:49.21 delay 2 seconds @14:27:51.24 Adding MM2 @14:27:51.32 AS.seek 0.524ml @14:28:09.62 delay 25 seconds @14:28:34.65 ARV.dial :PP4 @14:28:35.04 Adding PP4 @14:28:35.06 delay 2 seconds @14:28:37.14 AS.seek 0.53ml @14:28:43.32 delay 10 seconds @14:28:53.35 ARV.dial :air3 @14:28:53.73 adding ARV air @14:28:53.75 delay 2 seconds @14:28:55.83 AS.seek 0.536ml @14:29:02.05 delay 5 seconds @14:29:07.08 ARV.dial :carrier @14:29:08.29 delay 7 seconds @14:29:15.39 AS.seek 0.594ml @14:30:14.06 delay 5 seconds @14:30:19.09 ATV.dial :ASV @14:30:19.48 delay 2 seconds @14:30:21.58 AS.seek 0.6ml @14:30:27.87 delay 4 seconds @14:30:31.90 ATV.dial ARV @14:30:32.43 delay 2 seconds @14:30:34.46 ARV.dial :carrier @14:30:34.47 delay 2 seconds @14:30:36.56 AS.seek 0.65ml @14:31:27.06 delay 4 seconds @14:31:31.09 ATV.dial PCR @14:31:31.68 ASV.dial :waste1 @14:31:31.69 ARV.dial :air1 @14:31:32.07 Positioning Slug by 0.324ml @14:31:32.16 AS.seek 0.326ml @14:36:58.25 delay 5 seconds @14:37:03.28 ATV.dial ARV @14:37:04.03 ASV.dial :PCRlock @14:37:04.42 delay 5 seconds @14:37:09.45 Running ML Profile60C350 thermocycling @14:37:09.47 PCR.program Profile60C350 @14:37:09.58 Profile60C350 should complete in < 2:02:45 @14:37:09.59 PCR.run 2:38:26.25 @16:13:38.89 PCR.off @16:13:40.16 PCR for ML completed -- closed /var/log/esp/ML20may1714h525ml.pcr @16:13:40.23 Cleaning PCR @16:13:40.24 AFV.select :carrierSolution @16:13:40.35 delay 2 seconds @16:13:42.38 AS.reconfigure ASconfig @16:13:42.43 AS.seek 0.5ml @16:13:52.74 delay 1 second @16:13:53.76 AFV.select :holdingCoil @16:13:53.86 ASV.dial :waste1 @16:13:54.24 ATV.dial :air3 @16:13:55.48 AS.reconfigure ASslow1 @16:13:55.53 AS.seek 0.51ml @16:13:57.80 delay 2 seconds @16:13:59.83 ATV.dial :bleach @16:14:00.44 AS.seek 0.81ml @16:15:01.04 delay 3 seconds @16:15:04.07 ATV.dial :air3 @16:15:04.60 delay 3 seconds @16:15:07.69 AS.seek 0.835ml @16:15:12.99 delay 3 seconds @16:15:16.02 ATV.dial :bleach @16:15:16.63 AS.seek 0.82ml @16:15:19.93 delay 3 seconds @16:15:22.96 ARV.dial :waste @16:15:23.54 delay 3 seconds @16:15:26.57 ATV.dial :ARV @16:15:27.41 AS.reconfigure ASconfig @16:15:27.47 AS.seek 0.47ml @16:15:47.87 AFV.select :carrierSolution @16:15:47.98 delay 2 seconds @16:15:50.02 AS.seek 1ml @16:16:20.74 delay 1 second @16:16:21.76 AFV.select :holdingCoil @16:16:21.86 delay 1 second @16:16:22.89 AS.reconfigure ASfast @16:16:22.94 AS.seek 0ml @16:16:50.26 AFV.select :carrierSolution @16:16:50.37 delay 2 seconds @16:16:52.40 AS.reconfigure ASconfig @16:16:52.45 AS.seek 1ml @16:17:50.23 delay 1 second @16:17:51.25 AFV.select :holdingCoil @16:17:51.35 delay 1 second @16:17:52.38 AS.reconfigure ASfast @16:17:52.43 AS.seek 0ml @16:18:19.76 AFV.select :carrierSolution @16:18:19.87 delay 2 seconds @16:18:21.90 AS.reconfigure ASconfig @16:18:21.95 AS.seek 1ml @16:19:19.70 delay 1 second @16:19:20.72 AFV.select :holdingCoil @16:19:20.82 delay 1 second @16:19:21.85 AS.reconfigure ASfast @16:19:21.90 AS.seek 0ml @16:19:49.23 ARV.dial between carrier and air1 @16:19:49.72 ATV.dial :air3 @16:19:50.96 AS.reconfigure ASslow1 @16:19:51.01 AS.seek 0.01ml @16:19:53.31 delay 2 seconds @16:19:55.33 ATV.dial :bleach @16:19:55.94 AS.seek 0.81ml @16:22:37.08 delay 3 seconds @16:22:40.11 ATV.dial :air3 @16:22:40.64 delay 3 seconds @16:22:43.73 AS.seek 0.835ml @16:22:49.09 delay 3 seconds @16:22:52.12 ATV.dial :bleach @16:22:52.73 AS.seek 0.82ml @16:22:56.01 delay 3 seconds @16:22:59.03 ATV.dial PCR @16:22:59.42 AS.reconfigure ASconfig @16:22:59.47 AS.seek :empty @16:23:46.94 PCR.program :bleachProfile @16:23:46.99 PCR program should complete in < 2:45 @16:23:47.00 PCR.run 10:00 @16:27:01.56 PCR.off @16:27:01.59 AFV.select :carrierSolution @16:27:01.70 delay 2 seconds @16:27:03.74 AS.seek 1ml @16:28:01.55 delay 1 second @16:28:02.57 AFV.select :holdingCoil @16:28:02.67 delay 1 second @16:28:03.70 AS.reconfigure ASfast @16:28:03.75 AS.seek 0ml @16:28:31.11 delay 3 seconds @16:28:34.14 AFV.select :carrierSolution @16:28:34.24 delay 2 seconds @16:28:36.27 AS.reconfigure ASconfig @16:28:36.33 AS.seek 1ml @16:29:34.16 delay 1 second @16:29:35.18 AFV.select :holdingCoil @16:29:35.28 delay 1 second @16:29:36.31 AS.reconfigure ASfast @16:29:36.36 AS.seek 0ml @16:30:03.71 delay 3 seconds @16:30:06.74 AFV.select :carrierSolution @16:30:06.84 delay 2 seconds @16:30:08.87 AS.reconfigure ASconfig @16:30:08.93 AS.seek 1ml @16:31:06.68 delay 1 second @16:31:07.70 AFV.select :holdingCoil @16:31:07.80 delay 1 second @16:31:08.83 AS.reconfigure ASfast @16:31:08.88 AS.seek 0ml @16:31:36.22 delay 3 seconds @16:31:39.25 AFV.select :carrierSolution @16:31:39.35 delay 2 seconds @16:31:41.38 AS.reconfigure ASconfig @16:31:41.44 AS.seek 1ml @16:32:39.22 delay 1 second @16:32:40.24 AFV.select :holdingCoil @16:32:40.34 delay 1 second @16:32:41.37 AS.reconfigure ASfast @16:32:41.42 AS.seek 0ml @16:33:08.76 delay 3 seconds @16:33:11.79 AFV.select :carrierSolution @16:33:11.89 delay 2 seconds @16:33:13.92 AS.reconfigure ASconfig @16:33:13.98 AS.seek 1ml @16:34:11.79 delay 1 second @16:34:12.81 AFV.select :holdingCoil @16:34:12.91 delay 1 second @16:34:13.94 AS.reconfigure ASnorm @16:34:13.99 AS.seek 0ml @16:35:04.68 Setting up PCR for CG -- writing /var/log/esp/CG20may1716h525ml.pcr @16:35:04.70 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:35:04.71 Priming with carrier @16:35:04.72 ATV.dial ARV @16:35:05.30 ARV.dial :carrier @16:35:05.64 AS.seek 0.5ml @16:35:31.21 AFV.select :carrierSolution @16:35:31.32 delay 2 seconds @16:35:33.35 AS.reconfigure ASconfig @16:35:33.40 AS.seek 1ml @16:36:02.48 delay 1 second @16:36:03.50 AFV.select :holdingCoil @16:36:03.60 delay 1 second @16:36:04.63 ATV.dial :waste @16:36:06.20 AS.reconfigure ASnorm @16:36:06.25 AS.seek :empty @16:36:56.98 AFV.select :carrierSolution @16:36:57.09 delay 2 seconds @16:36:59.12 AS.reconfigure ASconfig @16:36:59.17 AS.seek 0.5ml @16:37:28.28 delay 1 second @16:37:29.30 AFV.select :holdingCoil @16:37:29.40 Building Slug @16:37:29.41 ATV.dial :ARV @16:37:30.96 delay 2 seconds @16:37:32.99 ARV.dial :air1 @16:37:33.38 delay 2 seconds @16:37:35.47 AS.reconfigure ASslow2 @16:37:35.52 AS.seek 0.506ml @16:37:41.98 delay 2 seconds @16:37:44.00 ARV.dial :MM1 @16:37:44.57 delay 2 seconds @16:37:46.60 Adding MM1 @16:37:46.67 AS.seek 0.524ml @16:38:04.96 delay 25 seconds @16:38:29.99 ARV.dial :PP2 @16:38:30.37 Adding PP2 @16:38:30.39 delay 2 seconds @16:38:32.47 AS.seek 0.53ml @16:38:38.74 delay 10 seconds @16:38:48.77 ARV.dial :air2 @16:38:49.14 adding ARV air @16:38:49.16 delay 2 seconds @16:38:51.24 AS.seek 0.536ml @16:38:57.62 delay 5 seconds @16:39:02.65 ARV.dial :carrier @16:39:03.85 delay 7 seconds @16:39:10.94 AS.seek 0.594ml @16:40:09.53 delay 5 seconds @16:40:15.19 ATV.dial :ASV @16:40:15.59 delay 2 seconds @16:40:17.68 AS.seek 0.6ml @16:40:23.93 delay 4 seconds @16:40:27.96 ATV.dial ARV @16:40:28.50 delay 2 seconds @16:40:30.53 ARV.dial :carrier @16:40:30.54 delay 2 seconds @16:40:32.63 AS.seek 0.65ml @16:41:23.32 delay 4 seconds @16:41:27.35 ATV.dial PCR @16:41:27.93 ASV.dial :waste1 @16:41:27.94 ARV.dial :air1 @16:41:28.34 Positioning Slug by 0.324ml @16:41:28.43 AS.seek 0.326ml @16:46:54.44 delay 5 seconds @16:46:59.47 ATV.dial ARV @16:47:00.22 ASV.dial :PCRlock @16:47:00.61 delay 5 seconds @16:47:05.64 Running CG Profile60C300 thermocycling @16:47:05.65 PCR.program Profile60C300 @16:47:05.76 Profile60C300 should complete in < 1:59:25 @16:47:05.77 PCR.run 2:34:16.25 @18:22:33.35 PCR.off @18:22:34.01 PCR for CG completed -- closed /var/log/esp/CG20may1716h525ml.pcr @18:22:34.08 Cleaning PCR @18:22:34.09 AFV.select :carrierSolution @18:22:34.20 delay 2 seconds @18:22:36.23 AS.reconfigure ASconfig @18:22:36.28 AS.seek 0.5ml @18:22:46.59 delay 1 second @18:22:47.61 AFV.select :holdingCoil @18:22:47.71 ASV.dial :waste1 @18:22:48.09 ATV.dial :air3 @18:22:49.33 AS.reconfigure ASslow1 @18:22:49.38 AS.seek 0.51ml @18:22:51.68 delay 2 seconds @18:22:53.70 ATV.dial :bleach @18:22:54.30 AS.seek 0.81ml @18:23:54.89 delay 3 seconds @18:23:57.92 ATV.dial :air3 @18:23:58.46 delay 3 seconds @18:24:01.55 AS.seek 0.835ml @18:24:07.49 delay 3 seconds @18:24:10.54 ATV.dial :bleach @18:24:11.14 AS.seek 0.82ml @18:24:14.43 delay 3 seconds @18:24:17.46 ARV.dial :waste @18:24:18.04 delay 3 seconds @18:24:21.07 ATV.dial :ARV @18:24:21.92 AS.reconfigure ASconfig @18:24:21.97 AS.seek 0.47ml @18:24:42.39 AFV.select :carrierSolution @18:24:42.50 delay 2 seconds @18:24:44.54 AS.seek 1ml @18:25:15.28 delay 1 second @18:25:16.30 AFV.select :holdingCoil @18:25:16.40 delay 1 second @18:25:17.43 AS.reconfigure ASfast @18:25:17.48 AS.seek 0ml @18:25:44.82 AFV.select :carrierSolution @18:25:44.93 delay 2 seconds @18:25:46.96 AS.reconfigure ASconfig @18:25:47.01 AS.seek 1ml @18:26:44.81 delay 1 second @18:26:45.83 AFV.select :holdingCoil @18:26:45.93 delay 1 second @18:26:46.96 AS.reconfigure ASfast @18:26:47.01 AS.seek 0ml @18:27:14.37 AFV.select :carrierSolution @18:27:14.48 delay 2 seconds @18:27:16.51 AS.reconfigure ASconfig @18:27:16.56 AS.seek 1ml @18:28:14.36 delay 1 second @18:28:15.38 AFV.select :holdingCoil @18:28:15.48 delay 1 second @18:28:16.51 AS.reconfigure ASfast @18:28:16.56 AS.seek 0ml @18:28:43.90 ARV.dial between carrier and air1 @18:28:44.37 ATV.dial :air3 @18:28:45.62 AS.reconfigure ASslow1 @18:28:45.67 AS.seek 0.01ml @18:28:48.00 delay 2 seconds @18:28:50.02 ATV.dial :bleach @18:28:50.63 AS.seek 0.81ml @18:31:31.77 delay 3 seconds @18:31:34.80 ATV.dial :air3 @18:31:35.34 delay 3 seconds @18:31:38.43 AS.seek 0.835ml @18:31:43.72 delay 3 seconds @18:31:46.75 ATV.dial :bleach @18:31:47.35 AS.seek 0.82ml @18:31:50.64 delay 3 seconds @18:31:53.67 ATV.dial PCR @18:31:54.06 AS.reconfigure ASconfig @18:31:54.11 AS.seek :empty @18:32:41.58 PCR.program :bleachProfile @18:32:41.63 PCR program should complete in < 2:45 @18:32:41.64 PCR.run 10:00 @18:35:55.58 PCR.off @18:35:55.61 AFV.select :carrierSolution @18:35:55.72 delay 2 seconds @18:35:57.76 AS.seek 1ml @18:36:55.58 delay 1 second @18:36:56.60 AFV.select :holdingCoil @18:36:56.70 delay 1 second @18:36:57.73 AS.reconfigure ASfast @18:36:57.78 AS.seek 0ml @18:37:25.11 delay 3 seconds @18:37:28.14 AFV.select :carrierSolution @18:37:28.24 delay 2 seconds @18:37:30.27 AS.reconfigure ASconfig @18:37:30.33 AS.seek 1ml @18:38:28.10 delay 1 second @18:38:29.12 AFV.select :holdingCoil @18:38:29.25 delay 1 second @18:38:30.28 AS.reconfigure ASfast @18:38:30.33 AS.seek 0ml @18:38:57.69 delay 3 seconds @18:39:00.72 AFV.select :carrierSolution @18:39:00.82 delay 2 seconds @18:39:02.85 AS.reconfigure ASconfig @18:39:02.91 AS.seek 1ml @18:40:00.73 delay 1 second @18:40:01.75 AFV.select :holdingCoil @18:40:01.85 delay 1 second @18:40:02.88 AS.reconfigure ASfast @18:40:02.93 AS.seek 0ml @18:40:30.27 delay 3 seconds @18:40:33.30 AFV.select :carrierSolution @18:40:33.40 delay 2 seconds @18:40:35.43 AS.reconfigure ASconfig @18:40:35.48 AS.seek 1ml @18:41:33.29 delay 1 second @18:41:34.31 AFV.select :holdingCoil @18:41:34.41 delay 1 second @18:41:35.44 AS.reconfigure ASfast @18:41:35.49 AS.seek 0ml @18:42:02.85 delay 3 seconds @18:42:05.88 AFV.select :carrierSolution @18:42:05.98 delay 2 seconds @18:42:08.61 AS.reconfigure ASconfig @18:42:08.69 AS.seek 1ml @18:43:06.52 delay 1 second @18:43:07.54 AFV.select :holdingCoil @18:43:07.64 delay 1 second @18:43:08.67 AS.reconfigure ASnorm @18:43:08.72 AS.seek 0ml @18:43:59.45 Setting up PCR for NM -- writing /var/log/esp/NM20may1718h525ml.pcr @18:43:59.47 Building PCR Slug from ASV with MM1 mix, PP1 primer @18:43:59.48 Priming with carrier @18:43:59.49 ATV.dial ARV @18:44:00.09 ARV.dial :carrier @18:44:00.44 AS.seek 0.5ml @18:44:25.96 AFV.select :carrierSolution @18:44:26.07 delay 2 seconds @18:44:28.10 AS.reconfigure ASconfig @18:44:28.15 AS.seek 1ml @18:44:57.17 delay 1 second @18:44:58.20 AFV.select :holdingCoil @18:44:58.31 delay 1 second @18:44:59.34 ATV.dial :waste @18:45:00.93 AS.reconfigure ASnorm @18:45:00.99 AS.seek :empty @18:45:51.73 AFV.select :carrierSolution @18:45:51.84 delay 2 seconds @18:45:53.87 AS.reconfigure ASconfig @18:45:53.92 AS.seek 0.5ml @18:46:23.04 delay 1 second @18:46:24.06 AFV.select :holdingCoil @18:46:24.16 Building Slug @18:46:24.17 ATV.dial :ARV @18:46:25.73 delay 2 seconds @18:46:27.76 ARV.dial :air1 @18:46:28.15 delay 2 seconds @18:46:30.24 AS.reconfigure ASslow2 @18:46:30.29 AS.seek 0.506ml @18:46:36.68 delay 2 seconds @18:46:38.70 ARV.dial :MM1 @18:46:39.26 delay 2 seconds @18:46:41.29 Adding MM1 @18:46:41.36 AS.seek 0.524ml @18:46:59.73 delay 25 seconds @18:47:24.76 ARV.dial :PP1 @18:47:25.14 Adding PP1 @18:47:25.16 delay 2 seconds @18:47:27.24 AS.seek 0.53ml @18:47:33.62 delay 10 seconds @18:47:43.65 ARV.dial :air1 @18:47:44.01 adding ARV air @18:47:44.03 delay 2 seconds @18:47:46.11 AS.seek 0.536ml @18:47:52.31 delay 5 seconds @18:47:57.34 ARV.dial :carrier @18:47:57.73 delay 7 seconds @18:48:04.82 AS.seek 0.594ml @18:49:03.29 delay 5 seconds @18:49:08.32 ATV.dial :ASV @18:49:08.72 delay 2 seconds @18:49:10.81 AS.seek 0.6ml @18:49:17.26 delay 4 seconds @18:49:21.29 ATV.dial ARV @18:49:21.81 delay 2 seconds @18:49:23.84 ARV.dial :carrier @18:49:23.85 delay 2 seconds @18:49:25.94 AS.seek 0.65ml @18:50:16.39 delay 4 seconds @18:50:20.42 ATV.dial PCR @18:50:21.00 ASV.dial :waste1 @18:50:21.01 ARV.dial :air1 @18:50:21.41 Positioning Slug by 0.324ml @18:50:21.50 AS.seek 0.326ml @18:55:47.53 delay 5 seconds @18:55:52.56 ATV.dial ARV @18:55:53.30 ASV.dial :PCRlock @18:55:53.69 delay 5 seconds @18:55:58.72 Running NM Profile60C300 thermocycling @18:55:58.73 PCR.program Profile60C300 @18:55:58.84 Profile60C300 should complete in < 1:59:25 @18:55:58.85 PCR.run 2:34:16.25 @20:31:22.83 PCR.off @20:31:23.50 PCR for NM completed -- closed /var/log/esp/NM20may1718h525ml.pcr @20:31:23.57 Cleaning PCR @20:31:23.58 AFV.select :carrierSolution @20:31:23.69 delay 2 seconds @20:31:25.72 AS.reconfigure ASconfig @20:31:25.77 AS.seek 0.5ml @20:31:36.07 delay 1 second @20:31:37.09 AFV.select :holdingCoil @20:31:37.19 ASV.dial :waste1 @20:31:37.57 ATV.dial :air3 @20:31:38.78 AS.reconfigure ASslow1 @20:31:38.83 AS.seek 0.51ml @20:31:41.13 delay 2 seconds @20:31:43.15 ATV.dial :bleach @20:31:43.75 AS.seek 0.81ml @20:32:44.32 delay 3 seconds @20:32:47.35 ATV.dial :air3 @20:32:47.89 delay 3 seconds @20:32:50.98 AS.seek 0.835ml @20:32:56.27 delay 3 seconds @20:32:59.30 ATV.dial :bleach @20:32:59.91 AS.seek 0.82ml @20:33:03.20 delay 3 seconds @20:33:06.22 ARV.dial :waste @20:33:06.81 delay 3 seconds @20:33:09.84 ATV.dial :ARV @20:33:10.68 AS.reconfigure ASconfig @20:33:10.74 AS.seek 0.47ml @20:33:31.14 AFV.select :carrierSolution @20:33:31.26 delay 2 seconds @20:33:33.31 AS.seek 1ml @20:33:57.73 Session Begun @20:33:59.39 -> can @20:34:04.04 delay 1 second @20:34:05.06 AFV.select :holdingCoil @20:34:05.18 delay 1 second @20:34:06.21 AS.reconfigure ASfast @20:34:06.29 AS.seek 0ml @20:34:09.00 -> exit @20:34:09.02 Session Ended @20:34:33.61 AFV.select :carrierSolution @20:34:33.72 delay 2 seconds @20:34:35.75 AS.reconfigure ASconfig @20:34:35.82 AS.seek 1ml @20:35:33.61 delay 1 second @20:35:34.63 AFV.select :holdingCoil @20:35:34.73 delay 1 second @20:35:35.76 AS.reconfigure ASfast @20:35:35.81 AS.seek 0ml @20:36:03.15 AFV.select :carrierSolution @20:36:03.26 delay 2 seconds @20:36:05.29 AS.reconfigure ASconfig @20:36:05.34 AS.seek 1ml @20:37:03.17 delay 1 second @20:37:04.19 AFV.select :holdingCoil @20:37:04.29 delay 1 second @20:37:05.32 AS.reconfigure ASfast @20:37:05.37 AS.seek 0ml @20:37:32.69 ARV.dial between carrier and air1 @20:37:33.17 ATV.dial :air3 @20:37:34.41 AS.reconfigure ASslow1 @20:37:34.46 AS.seek 0.01ml @20:37:36.72 delay 2 seconds @20:37:38.74 ATV.dial :bleach @20:37:39.99 AS.seek 0.81ml @20:40:21.13 delay 3 seconds @20:40:24.16 ATV.dial :air3 @20:40:24.70 delay 3 seconds @20:40:27.79 AS.seek 0.835ml @20:40:33.10 delay 3 seconds @20:40:36.13 ATV.dial :bleach @20:40:36.74 AS.seek 0.82ml @20:40:40.05 delay 3 seconds @20:40:43.08 ATV.dial PCR @20:40:43.47 AS.reconfigure ASconfig @20:40:43.52 AS.seek :empty @20:41:30.99 PCR.program :bleachProfile @20:41:31.05 PCR program should complete in < 2:45 @20:41:31.06 PCR.run 10:00 @20:44:44.99 PCR.off @20:44:45.02 AFV.select :carrierSolution @20:44:45.13 delay 2 seconds @20:44:47.17 AS.seek 1ml @20:45:45.00 delay 1 second @20:45:46.02 AFV.select :holdingCoil @20:45:46.12 delay 1 second @20:45:47.15 AS.reconfigure ASfast @20:45:47.20 AS.seek 0ml @20:46:14.50 delay 3 seconds @20:46:17.53 AFV.select :carrierSolution @20:46:17.63 delay 2 seconds @20:46:19.66 AS.reconfigure ASconfig @20:46:19.71 AS.seek 1ml @20:47:17.51 delay 1 second @20:47:18.53 AFV.select :holdingCoil @20:47:18.63 delay 1 second @20:47:19.66 AS.reconfigure ASfast @20:47:19.71 AS.seek 0ml @20:47:47.04 delay 3 seconds @20:47:50.07 AFV.select :carrierSolution @20:47:50.17 delay 2 seconds @20:47:52.20 AS.reconfigure ASconfig @20:47:52.26 AS.seek 1ml @20:48:50.05 delay 1 second @20:48:51.07 AFV.select :holdingCoil @20:48:51.17 delay 1 second @20:48:52.20 AS.reconfigure ASfast @20:48:52.25 AS.seek 0ml @20:49:19.59 delay 3 seconds @20:49:22.62 AFV.select :carrierSolution @20:49:22.72 delay 2 seconds @20:49:24.75 AS.reconfigure ASconfig @20:49:24.80 AS.seek 1ml @20:50:22.57 delay 1 second @20:50:23.59 AFV.select :holdingCoil @20:50:23.69 delay 1 second @20:50:24.72 AS.reconfigure ASfast @20:50:24.77 AS.seek 0ml @20:50:52.12 delay 3 seconds @20:50:55.15 AFV.select :carrierSolution @20:50:55.25 delay 2 seconds @20:50:57.28 AS.reconfigure ASconfig @20:50:57.37 AS.seek 1ml @20:51:55.20 delay 1 second @20:51:56.22 AFV.select :holdingCoil @20:51:56.32 delay 1 second @20:51:57.35 AS.reconfigure ASnorm @20:51:57.40 AS.seek 0ml @20:52:48.12 Setting up PCR for IPc -- writing /var/log/esp/IPc20may1720h525ml.pcr @20:52:48.13 Building PCR Slug from ASV with MM3 mix, PP6 primer @20:52:48.15 Priming with carrier @20:52:48.16 ATV.dial ARV @20:52:48.74 ARV.dial :carrier @20:52:49.08 AS.seek 0.5ml @20:53:14.63 AFV.select :carrierSolution @20:53:14.74 delay 2 seconds @20:53:16.77 AS.reconfigure ASconfig @20:53:16.82 AS.seek 1ml @20:53:45.89 delay 1 second @20:53:46.91 AFV.select :holdingCoil @20:53:47.01 delay 1 second @20:53:48.04 ATV.dial :waste @20:53:49.60 AS.reconfigure ASnorm @20:53:49.65 AS.seek :empty @20:54:40.42 AFV.select :carrierSolution @20:54:40.53 delay 2 seconds @20:54:42.56 AS.reconfigure ASconfig @20:54:42.61 AS.seek 0.5ml @20:55:11.68 delay 1 second @20:55:12.70 AFV.select :holdingCoil @20:55:12.80 Building Slug @20:55:12.81 ATV.dial :ARV @20:55:14.37 delay 2 seconds @20:55:16.40 ARV.dial :air1 @20:55:16.79 delay 2 seconds @20:55:18.88 AS.reconfigure ASslow2 @20:55:18.93 AS.seek 0.506ml @20:55:25.15 delay 2 seconds @20:55:27.17 ARV.dial :MM3 @20:55:28.17 delay 2 seconds @20:55:30.20 Adding MM3 @20:55:30.92 AS.seek 0.524ml @20:55:49.36 delay 25 seconds @20:56:14.39 ARV.dial :PP6 @20:56:14.78 Adding PP6 @20:56:14.80 delay 2 seconds @20:56:16.88 AS.seek 0.53ml @20:56:23.31 delay 10 seconds @20:56:33.34 ARV.dial :air1 @20:56:34.22 adding ARV air @20:56:34.24 delay 2 seconds @20:56:36.32 AS.seek 0.536ml @20:56:42.72 delay 5 seconds @20:56:47.75 ARV.dial :carrier @20:56:48.12 delay 7 seconds @20:56:55.21 AS.seek 0.594ml @20:57:53.67 delay 5 seconds @20:57:58.70 ATV.dial :ASV @20:57:59.09 delay 2 seconds @20:58:01.18 AS.seek 0.6ml @20:58:07.42 delay 4 seconds @20:58:11.45 ATV.dial ARV @20:58:11.98 delay 2 seconds @20:58:14.01 ARV.dial :carrier @20:58:14.02 delay 2 seconds @20:58:16.11 AS.seek 0.65ml @20:59:06.58 delay 4 seconds @20:59:10.61 ATV.dial PCR @20:59:11.20 ASV.dial :waste1 @20:59:11.21 ARV.dial :air1 @20:59:11.60 Positioning Slug by 0.324ml @20:59:11.69 AS.seek 0.326ml @21:04:37.80 delay 5 seconds @21:04:42.83 ATV.dial ARV @21:04:43.67 ASV.dial :PCRlock @21:04:44.05 delay 5 seconds @21:04:49.08 Running IPc ProfileIPc thermocycling @21:04:49.09 PCR.program ProfileIPc @21:04:49.20 ProfileIPc should complete in < 1:36:15 @21:04:49.21 PCR.run 2:05:18.75 @22:22:58.75 PCR.off @22:22:59.31 PCR for IPc completed -- closed /var/log/esp/IPc20may1720h525ml.pcr @22:22:59.38 Cleaning PCR @22:22:59.39 AFV.select :carrierSolution @22:22:59.50 delay 2 seconds @22:23:01.53 AS.reconfigure ASconfig @22:23:01.58 AS.seek 0.5ml @22:23:11.89 delay 1 second @22:23:12.91 AFV.select :holdingCoil @22:23:13.01 ASV.dial :waste1 @22:23:13.39 ATV.dial :air3 @22:23:14.61 AS.reconfigure ASslow1 @22:23:14.66 AS.seek 0.51ml @22:23:16.94 delay 2 seconds @22:23:18.97 ATV.dial :bleach @22:23:19.58 AS.seek 0.81ml @22:24:20.15 delay 3 seconds @22:24:23.18 ATV.dial :air3 @22:24:23.72 delay 3 seconds @22:24:26.81 AS.seek 0.835ml @22:24:32.09 delay 3 seconds @22:24:35.12 ATV.dial :bleach @22:24:35.72 AS.seek 0.82ml @22:24:39.03 delay 3 seconds @22:24:42.06 ARV.dial :waste @22:24:42.64 delay 3 seconds @22:24:45.67 ATV.dial :ARV @22:24:46.52 AS.reconfigure ASconfig @22:24:46.57 AS.seek 0.47ml @22:25:06.96 AFV.select :carrierSolution @22:25:07.08 delay 2 seconds @22:25:09.12 AS.seek 1ml @22:25:39.86 delay 1 second @22:25:40.88 AFV.select :holdingCoil @22:25:40.98 delay 1 second @22:25:42.01 AS.reconfigure ASfast @22:25:42.06 AS.seek 0ml @22:26:09.39 AFV.select :carrierSolution @22:26:09.50 delay 2 seconds @22:26:11.53 AS.reconfigure ASconfig @22:26:11.58 AS.seek 1ml @22:27:09.39 delay 1 second @22:27:10.41 AFV.select :holdingCoil @22:27:10.51 delay 1 second @22:27:11.54 AS.reconfigure ASfast @22:27:11.59 AS.seek 0ml @22:27:38.95 AFV.select :carrierSolution @22:27:39.06 delay 2 seconds @22:27:41.09 AS.reconfigure ASconfig @22:27:41.14 AS.seek 1ml @22:28:38.92 delay 1 second @22:28:39.94 AFV.select :holdingCoil @22:28:40.04 delay 1 second @22:28:41.07 AS.reconfigure ASfast @22:28:41.12 AS.seek 0ml @22:29:08.44 ARV.dial between carrier and air1 @22:29:08.92 ATV.dial :air3 @22:29:10.17 AS.reconfigure ASslow1 @22:29:10.22 AS.seek 0.01ml @22:29:12.55 delay 2 seconds @22:29:14.57 ATV.dial :bleach @22:29:15.18 AS.seek 0.81ml @22:31:56.32 delay 3 seconds @22:31:59.35 ATV.dial :air3 @22:31:59.88 delay 3 seconds @22:32:02.97 AS.seek 0.835ml @22:32:08.25 delay 3 seconds @22:32:11.28 ATV.dial :bleach @22:32:11.89 AS.seek 0.82ml @22:32:15.19 delay 3 seconds @22:32:18.22 ATV.dial PCR @22:32:18.61 AS.reconfigure ASconfig @22:32:18.66 AS.seek :empty @22:33:06.13 PCR.program :bleachProfile @22:33:06.18 PCR program should complete in < 2:45 @22:33:06.19 PCR.run 10:00 @22:36:20.75 PCR.off @22:36:20.78 AFV.select :carrierSolution @22:36:20.89 delay 2 seconds @22:36:22.93 AS.seek 1ml @22:37:20.76 delay 1 second @22:37:21.78 AFV.select :holdingCoil @22:37:21.88 delay 1 second @22:37:22.91 AS.reconfigure ASfast @22:37:22.96 AS.seek 0ml @22:37:50.30 delay 3 seconds @22:37:53.33 AFV.select :carrierSolution @22:37:53.43 delay 2 seconds @22:37:55.46 AS.reconfigure ASconfig @22:37:55.51 AS.seek 1ml @22:38:53.27 delay 1 second @22:38:54.29 AFV.select :holdingCoil @22:38:54.39 delay 1 second @22:38:55.42 AS.reconfigure ASfast @22:38:55.47 AS.seek 0ml @22:39:22.81 delay 3 seconds @22:39:25.84 AFV.select :carrierSolution @22:39:25.94 delay 2 seconds @22:39:27.97 AS.reconfigure ASconfig @22:39:28.03 AS.seek 1ml @22:40:25.82 delay 1 second @22:40:26.85 AFV.select :holdingCoil @22:40:26.95 delay 1 second @22:40:27.98 AS.reconfigure ASfast @22:40:28.03 AS.seek 0ml @22:40:55.34 delay 3 seconds @22:40:58.37 AFV.select :carrierSolution @22:40:58.47 delay 2 seconds @22:41:00.50 AS.reconfigure ASconfig @22:41:00.55 AS.seek 1ml @22:41:58.31 delay 1 second @22:41:59.33 AFV.select :holdingCoil @22:41:59.43 delay 1 second @22:42:00.46 AS.reconfigure ASfast @22:42:00.51 AS.seek 0ml @22:42:27.87 delay 3 seconds @22:42:30.90 AFV.select :carrierSolution @22:42:31.00 delay 2 seconds @22:42:33.03 AS.reconfigure ASconfig @22:42:33.09 AS.seek 1ml @22:43:30.84 delay 1 second @22:43:31.86 AFV.select :holdingCoil @22:43:31.96 delay 1 second @22:43:32.99 AS.reconfigure ASnorm @22:43:33.04 AS.seek 0ml @22:44:23.81 Setting up PCR for IPc2 -- writing /var/log/esp/IPc220may1722h525ml.pcr @22:44:23.82 Building PCR Slug from ASV with MM2 mix, PP3 primer @22:44:23.84 Priming with carrier @22:44:23.85 ATV.dial ARV @22:44:24.43 ARV.dial :carrier @22:44:24.77 AS.seek 0.5ml @22:44:50.33 AFV.select :carrierSolution @22:44:50.45 delay 2 seconds @22:44:52.48 AS.reconfigure ASconfig @22:44:52.53 AS.seek 1ml @22:45:21.61 delay 1 second @22:45:22.63 AFV.select :holdingCoil @22:45:22.73 delay 1 second @22:45:23.76 ATV.dial :waste @22:45:25.33 AS.reconfigure ASnorm @22:45:25.38 AS.seek :empty @22:46:16.12 AFV.select :carrierSolution @22:46:16.23 delay 2 seconds @22:46:18.26 AS.reconfigure ASconfig @22:46:18.31 AS.seek 0.5ml @22:46:47.42 delay 1 second @22:46:48.44 AFV.select :holdingCoil @22:46:48.55 Building Slug @22:46:48.56 ATV.dial :ARV @22:46:50.11 delay 2 seconds @22:46:52.14 ARV.dial :air1 @22:46:52.52 delay 2 seconds @22:46:54.61 AS.reconfigure ASslow2 @22:46:54.67 AS.seek 0.506ml @22:47:00.92 delay 2 seconds @22:47:02.94 ARV.dial :MM2 @22:47:04.34 delay 2 seconds @22:47:06.37 Adding MM2 @22:47:06.45 AS.seek 0.524ml @22:47:24.67 delay 25 seconds @22:47:49.70 ARV.dial :PP3 @22:47:50.08 Adding PP3 @22:47:50.10 delay 2 seconds @22:47:52.18 AS.seek 0.53ml @22:47:58.43 delay 10 seconds @22:48:08.49 ARV.dial :air2 @22:48:08.87 adding ARV air @22:48:08.89 delay 2 seconds @22:48:10.97 AS.seek 0.536ml @22:48:17.20 delay 5 seconds @22:48:22.23 ARV.dial :carrier @22:48:23.44 delay 7 seconds @22:48:30.53 AS.seek 0.594ml @22:49:29.08 delay 5 seconds @22:49:34.11 ATV.dial :ASV @22:49:34.50 delay 2 seconds @22:49:36.59 AS.seek 0.6ml @22:49:43.16 delay 4 seconds @22:49:47.19 ATV.dial ARV @22:49:48.45 delay 2 seconds @22:49:50.48 ARV.dial :carrier @22:49:50.49 delay 2 seconds @22:49:52.58 AS.seek 0.65ml @22:50:43.13 delay 4 seconds @22:50:47.16 ATV.dial PCR @22:50:47.75 ASV.dial :waste1 @22:50:47.76 ARV.dial :air1 @22:50:48.21 Positioning Slug by 0.324ml @22:50:48.30 AS.seek 0.326ml @22:56:14.49 delay 5 seconds @22:56:19.52 ATV.dial ARV @22:56:20.26 ASV.dial :PCRlock @22:56:20.65 delay 5 seconds @22:56:25.68 Running IPc2 ProfileIPc thermocycling @22:56:25.69 PCR.program ProfileIPc @22:56:25.80 ProfileIPc should complete in < 1:36:15 @22:56:25.81 PCR.run 2:05:18.75 @00:14:22.77CEST18-May-20 PCR.off @00:14:23.32 PCR for IPc2 completed -- closed /var/log/esp/IPc220may1722h525ml.pcr @00:14:23.34 Full MFB Decontamination @00:14:23.35 Decon extraction column and decon AIV @00:14:23.37 SPE.hold 2:46:40,55C @00:14:23.41 ASV.dial :SPEdelivery @00:14:24.05 delay 20 seconds @00:14:44.08 AFV.select :carrierSolution @00:14:44.18 delay 2 seconds @00:14:46.21 AS.reconfigure ASconfig @00:14:46.26 AS.seek 0.5ml @00:14:56.55 delay 1 second @00:14:57.57 AFV.select :holdingCoil @00:14:57.67 ATV.dial :air1 @00:15:00.09 AS.reconfigure ASslow1 @00:15:00.14 AS.seek 0.52ml @00:15:04.44 ATV.dial :ASV @00:15:06.09 ASV.dial :SPErecovery @00:15:06.74 AS.seek 0.62ml @00:15:27.14 ATV.dial :waste @00:15:28.98 AS.reconfigure ASnorm @00:15:29.03 AS.seek :empty @00:16:00.59 AFV.select :holdingCoil @00:16:00.60 ATV.dial :waste @00:16:00.62 AS.reconfigure ASconfig @00:16:00.67 AS.seek 0ml @00:16:00.97 AFV.select :carrierSolution @00:16:01.08 delay 2 seconds @00:16:03.12 AS.seek 0.5ml @00:16:32.13 delay 1 second @00:16:33.15 AFV.select :holdingCoil @00:16:33.25 ATV.dial :air3 @00:16:33.95 AS.reconfigure ASslow1 @00:16:34.00 AS.seek 0.52ml @00:16:38.30 ATV.dial :air3 @00:16:38.39 AS.seek 0.53ml @00:16:40.63 delay 2 seconds @00:16:42.65 ATV.dial :bleach @00:16:43.12 AS.seek 0.71ml @00:17:19.60 delay 3 seconds @00:17:22.63 ATV.dial :air3 @00:17:23.17 delay 3 seconds @00:17:26.26 AS.seek 0.735ml @00:17:31.64 delay 3 seconds @00:17:34.67 ATV.dial :bleach @00:17:35.27 AS.seek 0.72ml @00:17:38.53 delay 3 seconds @00:17:41.56 ATV.dial ASV @00:17:42.56 ASV.dial :SPErecovery @00:17:42.65 AS.seek 0.365ml @00:18:54.30 ATV.dial :waste @00:18:55.91 AS.reconfigure ASnorm @00:18:55.96 AS.seek :empty @00:19:14.77 ATV.dial :air3 @00:19:15.43 AS.reconfigure ASslow1 @00:19:15.48 AS.seek 0.009ml @00:19:17.86 delay 2 seconds @00:19:19.88 ATV.dial :bleach @00:19:20.34 AS.seek 0.579ml @00:21:15.22 delay 3 seconds @00:21:18.25 ATV.dial :air3 @00:21:18.79 delay 3 seconds @00:21:21.88 AS.seek 0.604ml @00:21:27.23 delay 3 seconds @00:21:30.26 ATV.dial :bleach @00:21:30.87 AS.seek 0.589ml @00:21:34.18 delay 3 seconds @00:21:37.21 ATV.dial :ASV @00:21:38.18 ASV.dial :wasteR @00:21:38.58 AS.seek 0ml @00:23:37.32 delay 1:00 @00:24:37.35 ATV.dial ASV @00:24:37.36 AFV.select :carrierSolution @00:24:37.47 delay 2 seconds @00:24:39.50 AS.reconfigure ASconfig @00:24:39.55 AS.seek 1ml @00:25:37.37 delay 1 second @00:25:38.39 AFV.select :holdingCoil @00:25:38.49 delay 1 second @00:25:39.52 ASV.dial :wasteR @00:25:39.54 AS.reconfigure ASslow1 @00:25:39.58 AS.seek 0ml @00:29:00.95 delay 10 seconds @00:29:10.98 ATV.dial :air3 @00:29:12.42 AS.seek 0.01ml @00:29:14.71 delay 2 seconds @00:29:16.73 ATV.dial :bleach @00:29:17.33 AS.seek 0.58ml @00:31:12.21 delay 3 seconds @00:31:15.24 ATV.dial :air3 @00:31:15.78 delay 3 seconds @00:31:18.87 AS.seek 0.605ml @00:31:24.18 delay 3 seconds @00:31:27.21 ATV.dial :bleach @00:31:27.82 AS.seek 0.59ml @00:31:31.51 delay 3 seconds @00:31:34.53 ATV.dial ASV @00:31:35.53 ASV.dial :SPEdelivery @00:31:35.91 AS.seek 0ml @00:33:34.81 delay 1:00 @00:34:34.84 AFV.select :carrierSolution @00:34:34.95 delay 2 seconds @00:34:36.98 AS.reconfigure ASconfig @00:34:37.03 AS.seek 1ml @00:35:34.79 delay 1 second @00:35:35.81 AFV.select :holdingCoil @00:35:35.91 delay 1 second @00:35:36.94 ATV.dial ASV @00:35:36.95 ASV.dial :SPEdelivery @00:35:36.97 AS.reconfigure ASslow1 @00:35:37.01 AS.seek 0ml @00:38:58.52 delay 10 seconds @00:39:08.55 ATV.dial :air2 @00:39:09.35 AS.reconfigure ASslow0 @00:39:09.40 AS.seek 0.8ml @00:40:30.16 delay 3 seconds @00:40:33.19 ATV.dial :ASV @00:40:34.11 ASV.dial :SPEdelivery @00:40:34.13 AS.reconfigure ASslow1 @00:40:34.18 AS.seek 0.03ml @00:43:09.30 delay 20 seconds @00:43:29.33 ATV.dial :waste,via: :air3 @00:43:30.97 AS.reconfigure ASconfig @00:43:31.02 AS.seek :empty @00:43:33.07 SPE.coast @00:43:33.16 Removing Bleach from Mixing Coil @00:43:33.18 AFV.select :carrierSolution @00:43:33.28 delay 2 seconds @00:43:35.32 AS.seek 0.5ml @00:44:04.43 delay 1 second @00:44:05.45 AFV.select :holdingCoil @00:44:05.55 ATV.dial :ASV @00:44:07.16 ASV.dial :SPErecovery @00:44:07.81 AS.reconfigure ASslow1 @00:44:07.86 AS.seek 0.73ml @00:44:54.38 ASV.dial :wasteD @00:44:54.94 AS.reconfigure ASnorm @00:44:54.99 AS.seek 0ml @00:45:32.13 AFV.select :carrierSolution @00:45:32.24 delay 2 seconds @00:45:34.27 AS.reconfigure ASconfig @00:45:34.32 AS.seek 0.5ml @00:46:03.35 delay 1 second @00:46:04.37 AFV.select :holdingCoil @00:46:04.47 ATV.dial ASV @00:46:04.48 ASV.dial :SPErecovery @00:46:05.12 AS.reconfigure ASslow0 @00:46:05.17 AS.seek 0.3ml @00:46:25.57 ATV.dial :air2 @00:46:26.57 AS.reconfigure ASslow1 @00:46:26.62 AS.seek 0.32ml @00:46:30.95 ATV.dial ASV @00:46:31.95 AS.reconfigure ASslow0 @00:46:32.00 AS.seek 0.57ml @00:46:57.47 ASV.dial :wasteD @00:46:58.04 AS.reconfigure ASnorm @00:46:58.10 AS.seek 0ml @00:47:27.16 AFV.select :carrierSolution @00:47:27.27 delay 2 seconds @00:47:29.30 AS.reconfigure ASconfig @00:47:29.35 AS.seek 0.5ml @00:47:58.38 delay 1 second @00:47:59.40 AFV.select :holdingCoil @00:47:59.50 ATV.dial ASV @00:47:59.51 ASV.dial :SPErecovery @00:48:00.15 AS.reconfigure ASslow0 @00:48:00.20 AS.seek 0.3ml @00:48:20.65 ATV.dial :air2 @00:48:21.65 AS.reconfigure ASslow1 @00:48:21.70 AS.seek 0.32ml @00:48:26.02 ATV.dial ASV @00:48:27.01 AS.reconfigure ASslow0 @00:48:27.06 AS.seek 0.57ml @00:48:52.51 ASV.dial :wasteD @00:48:53.08 AS.reconfigure ASnorm @00:48:53.13 AS.seek 0ml @00:49:22.16 ATV.dial :air2 @00:49:23.08 AS.reconfigure ASslow0 @00:49:23.14 AS.seek 0.3ml @00:49:53.63 ATV.dial :ASV @00:49:54.55 ASV.dial :wasteD @00:49:54.57 AS.reconfigure ASconfig @00:49:54.61 AS.seek 0.05ml @00:50:09.32 ATV.dial :waste @00:50:11.08 AS.seek 0ml @00:50:14.87 ASV.dial :SPEdelivery @00:50:15.84 deconLysateINT @00:50:15.85 ATV.dial :air3 @00:50:16.51 AS.reconfigure ASslow1 @00:50:16.56 AS.seek 0.01ml @00:50:18.88 delay 2 seconds @00:50:20.90 ATV.dial :bleach @00:50:21.36 AS.seek 0.96ml @00:53:32.67 delay 3 seconds @00:53:35.70 ATV.dial :air3 @00:53:36.24 delay 3 seconds @00:53:39.33 AS.seek 0.985ml @00:53:44.62 delay 3 seconds @00:53:47.65 ATV.dial :bleach @00:53:48.25 AS.seek 0.97ml @00:53:51.56 delay 3 seconds @00:53:54.59 ATV.dial AIV @00:53:55.79 AIV.dial :coilToMFB @00:53:55.81 AWV.select :waste @00:53:55.82 AFV.select :carrierSolution @00:53:55.93 delay 2 seconds @00:53:57.96 AS.reconfigure ASconfig @00:53:58.01 AS.seek 1ml @00:54:00.07 delay 1 second @00:54:01.09 AFV.select :holdingCoil @00:54:01.19 delay 1 second @00:54:02.22 AS.reconfigure ASslow0 @00:54:02.27 AS.seek :empty @00:55:43.24 AFV.select :carrierSolution @00:55:43.35 delay 2 seconds @00:55:45.38 AS.reconfigure ASconfig @00:55:45.43 AS.seek 1ml @00:56:43.29 delay 1 second @00:56:44.31 AFV.select :holdingCoil @00:56:44.41 delay 1 second @00:56:45.44 AS.reconfigure ASslow0 @00:56:45.49 AS.seek :empty @00:58:26.43 AFV.select :carrierSolution @00:58:26.54 delay 2 seconds @00:58:28.57 AS.reconfigure ASconfig @00:58:28.62 AS.seek 1ml @00:59:26.45 delay 1 second @00:59:27.47 AFV.select :holdingCoil @00:59:27.57 delay 1 second @00:59:28.60 AS.reconfigure ASslow0 @00:59:28.65 AS.seek :empty @01:01:09.65 ATV.dial :air2 @01:01:10.30 AS.reconfigure ASslow1 @01:01:10.35 AS.seek 0.009ml @01:01:12.64 AS.reconfigure ASslow0 @01:01:12.69 AS.seek 1ml @01:02:52.62 ATV.dial AIV @01:02:53.46 AS.seek 0.02ml @01:04:32.34 ATV.dial :waste @01:04:33.90 AS.seek 0ml @01:04:36.20 AWV.select :air @01:04:36.36 Cleaning PCR @01:04:36.37 AFV.select :carrierSolution @01:04:36.48 delay 2 seconds @01:04:38.51 AS.reconfigure ASconfig @01:04:38.56 AS.seek 0.5ml @01:05:07.59 delay 1 second @01:05:08.61 AFV.select :holdingCoil @01:05:08.72 ASV.dial :waste1 @01:05:09.09 ATV.dial :air3 @01:05:09.76 AS.reconfigure ASslow1 @01:05:09.81 AS.seek 0.51ml @01:05:12.08 delay 2 seconds @01:05:14.10 ATV.dial :bleach @01:05:14.56 AS.seek 0.81ml @01:06:15.14 delay 3 seconds @01:06:18.17 ATV.dial :air3 @01:06:18.70 delay 3 seconds @01:06:21.79 AS.seek 0.835ml @01:06:27.07 delay 3 seconds @01:06:30.10 ATV.dial :bleach @01:06:30.71 AS.seek 0.82ml @01:06:34.02 delay 3 seconds @01:06:37.05 ARV.dial :waste @01:06:37.63 delay 3 seconds @01:06:40.66 ATV.dial :ARV @01:06:41.51 AS.reconfigure ASconfig @01:06:41.56 AS.seek 0.47ml @01:07:01.97 AFV.select :carrierSolution @01:07:02.08 delay 2 seconds @01:07:04.12 AS.seek 1ml @01:07:34.96 delay 1 second @01:07:35.98 AFV.select :holdingCoil @01:07:36.08 delay 1 second @01:07:37.11 AS.reconfigure ASfast @01:07:37.16 AS.seek 0ml @01:08:04.49 AFV.select :carrierSolution @01:08:04.60 delay 2 seconds @01:08:06.63 AS.reconfigure ASconfig @01:08:06.68 AS.seek 1ml @01:09:05.11 delay 1 second @01:09:06.13 AFV.select :holdingCoil @01:09:06.23 delay 1 second @01:09:07.26 AS.reconfigure ASfast @01:09:07.31 AS.seek 0ml @01:09:34.61 AFV.select :carrierSolution @01:09:34.72 delay 2 seconds @01:09:36.75 AS.reconfigure ASconfig @01:09:36.80 AS.seek 1ml @01:10:34.55 delay 1 second @01:10:35.57 AFV.select :holdingCoil @01:10:35.67 delay 1 second @01:10:36.70 AS.reconfigure ASfast @01:10:36.75 AS.seek 0ml @01:11:04.11 ARV.dial between carrier and air1 @01:11:04.58 ATV.dial :air3 @01:11:05.82 AS.reconfigure ASslow1 @01:11:05.87 AS.seek 0.01ml @01:11:08.21 delay 2 seconds @01:11:10.23 ATV.dial :bleach @01:11:10.84 AS.seek 0.81ml @01:13:51.94 delay 3 seconds @01:13:54.97 ATV.dial :air3 @01:13:55.51 delay 3 seconds @01:13:58.60 AS.seek 0.835ml @01:14:03.92 delay 3 seconds @01:14:06.95 ATV.dial :bleach @01:14:07.56 AS.seek 0.82ml @01:14:10.87 delay 3 seconds @01:14:13.90 ATV.dial PCR @01:14:14.29 AS.reconfigure ASconfig @01:14:14.34 AS.seek :empty @01:15:01.90 PCR.program :bleachProfile @01:15:01.96 PCR program should complete in < 2:45 @01:15:01.97 PCR.run 10:00 @01:18:31.06 PCR.off @01:18:31.08 AFV.select :carrierSolution @01:18:31.19 delay 2 seconds @01:18:33.23 AS.seek 1ml @01:19:31.06 delay 1 second @01:19:32.08 AFV.select :holdingCoil @01:19:32.18 delay 1 second @01:19:33.21 AS.reconfigure ASfast @01:19:33.26 AS.seek 0ml @01:20:00.57 delay 3 seconds @01:20:03.60 AFV.select :carrierSolution @01:20:03.70 delay 2 seconds @01:20:05.73 AS.reconfigure ASconfig @01:20:05.79 AS.seek 1ml @01:21:03.59 delay 1 second @01:21:04.61 AFV.select :holdingCoil @01:21:04.71 delay 1 second @01:21:05.74 AS.reconfigure ASfast @01:21:05.79 AS.seek 0ml @01:21:33.12 delay 3 seconds @01:21:36.15 AFV.select :carrierSolution @01:21:36.25 delay 2 seconds @01:21:38.28 AS.reconfigure ASconfig @01:21:38.34 AS.seek 1ml @01:22:36.12 delay 1 second @01:22:37.14 AFV.select :holdingCoil @01:22:37.24 delay 1 second @01:22:38.27 AS.reconfigure ASfast @01:22:38.32 AS.seek 0ml @01:23:05.67 delay 3 seconds @01:23:08.70 AFV.select :carrierSolution @01:23:08.80 delay 2 seconds @01:23:10.83 AS.reconfigure ASconfig @01:23:10.89 AS.seek 1ml @01:24:08.69 delay 1 second @01:24:09.71 AFV.select :holdingCoil @01:24:09.81 delay 1 second @01:24:10.84 AS.reconfigure ASfast @01:24:10.89 AS.seek 0ml @01:24:38.23 delay 3 seconds @01:24:41.26 AFV.select :carrierSolution @01:24:41.36 delay 2 seconds @01:24:43.40 AS.reconfigure ASconfig @01:24:43.45 AS.seek 1ml @01:25:41.19 delay 1 second @01:25:42.21 AFV.select :holdingCoil @01:25:42.31 delay 1 second @01:25:43.34 AS.reconfigure ASnorm @01:25:43.39 AS.seek 0ml @01:26:34.12 AFV.select :carrierSolution @01:26:34.23 delay 2 seconds @01:26:36.26 AS.reconfigure ASconfig @01:26:36.31 AS.seek 1ml @01:27:34.13 delay 1 second @01:27:35.15 AFV.select :holdingCoil @01:27:35.25 delay 1 second @01:27:36.28 ATV.dial :waste @01:27:37.44 AS.reconfigure ASnorm @01:27:37.50 AS.seek 0.5ml @01:28:03.06 ATV.dial :waste2 @01:28:04.28 AS.seek 0ml @01:28:30.37 AS.coast @01:28:30.43 SS.coast @01:28:30.46 CC.coast @01:28:30.50 CS.coast @01:28:30.55 PC.coast @01:28:30.59 PS.coast @01:28:30.63 Forearm.coast @01:28:30.67 Elbow.coast @01:28:31.06 Gate.power :core,:OFF @01:28:31.08 Gate.power :raw,:OFF @01:28:31.09 Shutdown @01:28:31.10 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @08:19:01.33 Session Begun @08:20:01.34 -> ESP.wakeup @08:20:01.40 Powering core bus @08:20:06.13 Gate.power :raw,:ON @08:20:06.84 SPE.coast @08:20:06.93 SPE.configure SPEconfig @08:20:07.06 AS.configure ASconfig @08:20:07.19 SS.configure SSconfig @08:20:07.33 CC.configure CCconfig @08:20:07.46 CS.configure CSconfig @08:20:07.60 PC.configure PCconfig @08:20:07.73 PS.configure PSconfig @08:20:07.89 Forearm.configure LooseForearm @08:20:08.01 Elbow.configure LooseElbow @08:20:08.17 SC.configure SCloose @08:20:08.31 SE.configure SEconfig @08:20:08.38 CH.coast @08:20:08.47 CH.configure CHconfig @08:20:08.53 PH.coast @08:20:08.63 PH.configure PHconfig @08:20:08.69 PCR.off @08:20:08.75 PCR.pidtemp= 50,0.1,0,300,25,25 @08:20:08.79 PCR.caltemp= 2.8814,0.0372,0 @08:20:08.84 All dwarf controllers running firmware version 3.75 @08:20:08.99 Valve.reset @08:20:09.19 PCR.off @08:20:09.24 PCR.pidtemp= 50,0.1,0,300,25,25 @08:20:09.29 PCR.caltemp= 2.8814,0.0372,0 @08:20:09.55 PSV.dial :air @08:20:09.57 Intake.select :close @08:20:09.60 Exhaust.select :close @08:20:09.68 CTV.dial :air @08:20:09.85 CSV.dial :waste1 @08:20:09.89 Forearm.reconfigure LooseForearm @08:20:10.04 SSV.dial :spit,avoiding: :exhaust @08:20:10.07 Forearm.home.seek :retract @08:20:10.25 PS.reconfigure PSconfig @08:20:10.35 PS.home.seek 0ml @08:20:10.93 Hand.close @08:20:10.97 Elbow.jog 1500 @08:20:11.14 CS.reconfigure CSfast @08:20:11.18 SS.reconfigure SSconfig @08:20:11.31 CS.home.seek 0ml @08:20:11.34 SS.home.seek 0ml @08:20:11.76 Elbow.reconfigure LooseElbow @08:20:11.83 Elbow.home.seek :clear @08:20:15.30 SE.reconfigure SEconfig @08:20:15.37 SE.home.seek :down @08:20:16.01 SC.reconfigure SCsloppy @08:20:16.09 SC.home.seek :initial @08:20:18.31 Forearm.seek :retract @08:20:18.42 Elbow.seek :hammer @08:20:19.48 PS.coast @08:20:19.80 Forearm.reconfigure TightForearm @08:20:19.87 Forearm.seek 5 + 350 counts @08:20:21.49 Forearm.seek :retract @08:20:22.74 PC.reconfigure PCconfig @08:20:22.77 CC.reconfigure CCconfig @08:20:22.90 PC.home.seek :open @08:20:22.93 CC.home.seek :open @08:20:49.98 -> ATV.ARV @08:20:50.00 ATV.dial ARV @08:21:16.86 -> ARV.air3 @08:21:16.88 ARV.dial :air3 @08:21:32.54 -> AS.pull 0.03 @08:21:32.63 Slide::Error in bkha -- Analytical Syringe Not yet Homed! @08:21:49.50 -> MFB.ready! @08:21:49.60 Readying the MFB @08:21:49.61 Homing MFB Syringe @08:21:49.64 AFV.select :holdingCoil @08:21:49.84 ATV.dial :air2 @08:21:51.02 AS.reconfigure ASconfig @08:21:51.09 AS.home.seek :home @08:21:56.62 AFV.select :carrierSolution @08:21:56.75 delay 2 seconds @08:21:58.79 AS.seek 1ml @08:22:51.68 delay 1 second @08:22:52.70 AFV.select :holdingCoil @08:22:52.83 delay 1 second @08:22:53.86 ATV.dial :waste @08:22:54.89 AS.reconfigure ASnorm @08:22:54.97 AS.home.seek :empty @08:23:47.54 ATV.dial :waste @08:23:47.56 ARV.dial between carrier and air1 @08:23:48.90 ASV.dial :waste1 @08:24:06.46 -> ATV.ARV @08:24:06.48 ATV.dial ARV @08:25:23.79 -> ARV.air3 @08:25:23.81 ARV.dial :air3 @08:26:01.46 -> AS.pull 0.03 @08:26:01.57 AS.reconfigure ASconfig @08:26:01.64 AS.seek 0.029ml @08:26:40.18 -> ARV.PP5 @08:26:40.20 ARV.dial :PP5 @08:27:20.82 -> AS.pull 0.02, ASslow1 @08:27:20.93 AS.reconfigure ASslow1 @08:27:21.00 AS.seek 0.049ml @08:27:43.86 -> ARV.air3 @08:27:43.88 ARV.dial :air3 @08:28:12.02 -> AS.pull 0.03, ASslow2 @08:28:12.13 AS.reconfigure ASslow2 @08:28:12.20 AS.seek 0.079ml @08:28:50.69 -> ARV.carrier @08:28:50.71 ARV.dial :carrier @08:29:08.42 -> AS.pull 0.05, ASslow2 @08:29:08.53 AS.seek 0.129ml @08:30:05.87 -> ARV @08:30:08.17 -> ATV @08:30:37.87 -> ARV.air3 @08:30:37.89 ARV.dial :air3 @08:30:50.03 -> AS.pull 0.03 @08:30:50.14 AS.reconfigure ASconfig @08:30:50.21 AS.seek 0.159ml @08:31:03.15 -> ARV.MM3 @08:31:03.17 ARV.dial :MM3 @08:31:26.59 -> AS.pull 0.05, ASslow2 @08:31:26.69 AS.reconfigure ASslow2 @08:31:26.77 AS.seek 0.209ml @08:35:30.24 -> ARV.air3 @08:35:30.26 ARV.dial :air3 @08:35:44.83 -> AS.pull 0.03, ASslow2 @08:35:44.94 AS.seek 0.239ml @08:36:50.01 -> ATV.legend @08:39:19.54 -> ARV.carrier @08:39:19.56 ARV.dial :carrier @08:39:24.34 -> ATV @08:39:40.66 -> AS.pull 0.05, ASslow2 @08:39:40.77 AS.seek 0.289ml @08:40:38.00 -> ATV.waste @08:40:38.02 ATV.dial :waste @08:40:45.68 -> AS.empty @08:40:45.70 AS.reconfigure ASconfig @08:40:45.80 AS.seek :empty @08:41:54.20 -> pcrSlug(:air1,:air3,:air3,:air3) @08:41:54.22 Building PCR Slug from air1 with air3 mix, air3 primer @08:41:54.26 Priming with carrier @08:41:54.29 ATV.dial ARV @08:41:55.98 ARV.dial :carrier @08:41:56.09 AS.reconfigure ASnorm @08:41:56.16 AS.seek 0.499ml @08:42:21.74 AFV.select :carrierSolution @08:42:21.87 delay 2 seconds @08:42:23.90 AS.reconfigure ASconfig @08:42:23.97 AS.seek 1ml @08:42:53.67 delay 1 second @08:42:54.69 AFV.select :holdingCoil @08:42:54.82 delay 1 second @08:42:55.85 ATV.dial :waste @08:42:57.43 AS.reconfigure ASnorm @08:42:57.52 AS.seek :empty @08:43:48.31 AFV.select :carrierSolution @08:43:48.45 delay 2 seconds @08:43:50.48 AS.reconfigure ASconfig @08:43:50.56 AS.seek 0.5ml @08:44:19.67 delay 1 second @08:44:20.69 AFV.select :holdingCoil @08:44:20.82 Building Slug @08:44:20.83 ATV.dial :ARV @08:44:22.53 delay 2 seconds @08:44:24.56 ARV.dial :air1 @08:44:24.97 delay 2 seconds @08:44:27.06 AS.reconfigure ASslow2 @08:44:27.13 AS.seek 0.506ml @08:44:33.32 delay 2 seconds @08:44:35.34 ARV.dial :air3 @08:44:36.78 delay 2 seconds @08:44:38.81 Adding air3 @08:44:38.91 AS.seek 0.524ml @08:44:57.28 delay 25 seconds @08:45:22.31 ARV.dial :air3 @08:45:22.33 Adding air3 @08:45:22.35 delay 2 seconds @08:45:24.46 AS.seek 0.53ml @08:45:30.87 delay 10 seconds @08:45:40.90 ARV.dial :air3 @08:45:40.92 adding ARV air @08:45:40.95 delay 2 seconds @08:45:43.04 AS.seek 0.536ml @08:45:49.48 delay 5 seconds @08:45:54.51 ARV.dial :carrier @08:45:55.73 delay 7 seconds @08:46:02.82 AS.seek 0.594ml @08:47:01.29 delay 5 seconds @08:47:06.32 ATV.dial :air1 @08:47:08.11 delay 2 seconds @08:47:10.20 AS.seek 0.6ml @08:47:16.47 delay 4 seconds @08:47:20.50 ATV.dial ARV @08:47:22.13 delay 2 seconds @08:47:24.16 ARV.dial :carrier @08:47:24.17 delay 2 seconds @08:47:26.27 AS.seek 0.65ml @08:48:16.76 delay 4 seconds @08:48:20.79 ATV.dial PCR @08:48:21.37 ASV.dial :waste1 @08:48:21.39 ARV.dial :air1 @08:48:21.81 Positioning Slug by 0.324ml @08:48:21.92 AS.seek 0.326ml @08:53:47.95 delay 5 seconds @08:53:52.98 ATV.dial ARV @08:53:53.74 ASV.dial :PCRlock @09:09:45.85 Session Ended @09:12:32.82 Session Begun @09:12:40.14 -> showlog 30 @09:13:07.34 -> PCR.temp=60 @09:13:07.36 PCR.temp= 60 @09:13:22.70 -> PCR.program :diodeCalFAM @09:13:22.72 PCR.program :diodeCalFAM @09:13:22.92 PCR program should complete in < 1:04 @09:13:27.82 -> PCR.run @09:13:27.85 PCR.run 3:00:00 @09:13:47.33 PCR.off @09:13:53.34 -> PCR.sample @09:14:18.39 -> PCR.sample @09:16:50.89 -> pcrDecon @09:16:50.99 Cleaning PCR @09:16:51.00 AFV.select :carrierSolution @09:16:51.16 delay 2 seconds @09:16:53.19 AS.reconfigure ASconfig @09:16:53.27 AS.seek 0.5ml @09:17:03.58 delay 1 second @09:17:04.60 AFV.select :holdingCoil @09:17:04.72 ASV.dial :waste1 @09:17:05.13 ATV.dial :air3 @09:17:06.37 AS.reconfigure ASslow1 @09:17:06.44 AS.seek 0.51ml @09:17:08.73 delay 2 seconds @09:17:10.75 ATV.dial :bleach @09:17:11.39 AS.seek 0.81ml @09:18:12.02 delay 3 seconds @09:18:15.05 ATV.dial :air3 @09:18:15.60 delay 3 seconds @09:18:18.69 AS.seek 0.835ml @09:18:24.03 delay 3 seconds @09:18:27.06 ATV.dial :bleach @09:18:27.68 AS.seek 0.82ml @09:18:30.96 delay 3 seconds @09:18:33.99 ARV.dial :waste @09:18:34.60 delay 3 seconds @09:18:37.63 ATV.dial :ARV @09:18:38.51 AS.reconfigure ASconfig @09:18:38.58 AS.seek 0.47ml @09:18:59.02 AFV.select :carrierSolution @09:18:59.15 delay 2 seconds @09:19:01.19 AS.seek 1ml @09:19:31.96 delay 1 second @09:19:32.98 AFV.select :holdingCoil @09:19:33.11 delay 1 second @09:19:34.14 AS.reconfigure ASfast @09:19:34.22 AS.seek 0ml @09:20:01.57 AFV.select :carrierSolution @09:20:01.70 delay 2 seconds @09:20:03.73 AS.reconfigure ASconfig @09:20:03.80 AS.seek 1ml @09:21:01.58 delay 1 second @09:21:02.60 AFV.select :holdingCoil @09:21:02.73 delay 1 second @09:21:03.76 AS.reconfigure ASfast @09:21:03.84 AS.seek 0ml @09:21:31.21 AFV.select :carrierSolution @09:21:31.34 delay 2 seconds @09:21:33.37 AS.reconfigure ASconfig @09:21:33.44 AS.seek 1ml @09:22:31.79 delay 1 second @09:22:32.81 AFV.select :holdingCoil @09:22:32.94 delay 1 second @09:22:33.97 AS.reconfigure ASfast @09:22:34.05 AS.seek 0ml @09:23:01.40 ARV.dial between carrier and air1 @09:23:01.90 ATV.dial :air3 @09:23:03.14 AS.reconfigure ASslow1 @09:23:03.22 AS.seek 0.01ml @09:23:05.54 delay 2 seconds @09:23:07.56 ATV.dial :bleach @09:23:08.17 AS.seek 0.81ml @09:25:49.34 delay 3 seconds @09:25:52.37 ATV.dial :air3 @09:25:52.90 delay 3 seconds @09:25:55.99 AS.seek 0.835ml @09:26:01.30 delay 3 seconds @09:26:04.33 ATV.dial :bleach @09:26:04.95 AS.seek 0.82ml @09:26:08.26 delay 3 seconds @09:26:11.29 ATV.dial PCR @09:26:11.71 AS.reconfigure ASconfig @09:26:11.79 AS.seek :empty @09:26:59.26 PCR.program :bleachProfile @09:26:59.33 PCR program should complete in < 2:45 @09:26:59.35 PCR.run 10:00 @09:30:31.50 PCR.off @09:30:31.55 AFV.select :carrierSolution @09:30:31.68 delay 2 seconds @09:30:33.72 AS.seek 1ml @09:31:31.58 delay 1 second @09:31:32.60 AFV.select :holdingCoil @09:31:32.73 delay 1 second @09:31:33.76 AS.reconfigure ASfast @09:31:33.84 AS.seek 0ml @09:32:01.19 delay 3 seconds @09:32:04.22 AFV.select :carrierSolution @09:32:04.35 delay 2 seconds @09:32:06.38 AS.reconfigure ASconfig @09:32:06.46 AS.seek 1ml @09:33:04.25 delay 1 second @09:33:05.27 AFV.select :holdingCoil @09:33:05.40 delay 1 second @09:33:06.43 AS.reconfigure ASfast @09:33:06.51 AS.seek 0ml @09:33:33.87 delay 3 seconds @09:33:36.90 AFV.select :carrierSolution @09:33:37.03 delay 2 seconds @09:33:39.06 AS.reconfigure ASconfig @09:33:39.14 AS.seek 1ml @09:34:36.95 delay 1 second @09:34:37.97 AFV.select :holdingCoil @09:34:38.10 delay 1 second @09:34:39.13 AS.reconfigure ASfast @09:34:39.21 AS.seek 0ml @09:35:06.55 delay 3 seconds @09:35:09.58 AFV.select :carrierSolution @09:35:09.71 delay 2 seconds @09:35:11.74 AS.reconfigure ASconfig @09:35:11.82 AS.seek 1ml @09:36:09.63 delay 1 second @09:36:10.65 AFV.select :holdingCoil @09:36:10.78 delay 1 second @09:36:11.81 AS.reconfigure ASfast @09:36:11.89 AS.seek 0ml @09:36:39.25 delay 3 seconds @09:36:42.28 AFV.select :carrierSolution @09:36:42.41 delay 2 seconds @09:36:44.44 AS.reconfigure ASconfig @09:36:44.51 AS.seek 1ml @09:37:42.33 delay 1 second @09:37:43.35 AFV.select :holdingCoil @09:37:43.48 delay 1 second @09:37:44.51 AS.reconfigure ASnorm @09:37:44.59 AS.seek 0ml @09:39:04.62 -> ESP.shutdown @09:39:04.66 AS.coast @09:39:04.69 Asleep / Shutdown @09:39:04.78 SS.coast @09:39:04.85 CC.coast @09:39:04.91 CS.coast @09:39:04.97 PC.coast @09:39:05.04 Forearm.coast @09:39:05.10 Elbow.coast @09:39:05.17 SC.coast @09:39:05.23 SE.coast @09:39:05.65 Gate.power :core,:OFF @09:39:05.67 Gate.power :raw,:OFF @09:39:05.70 Shutdown @09:39:09.01 -> exit @09:39:09.03 Session Ended @10:01:11.17
Powering core bus @10:01:15.88 Gate.power :raw,:ON @10:01:16.53 SPE.coast @10:01:16.60 SPE.configure SPEconfig @10:01:16.69 AS.configure ASconfig @10:01:16.80 SS.configure SSconfig @10:01:16.89 CC.configure CCconfig @10:01:16.99 CS.configure CSconfig @10:01:17.09 PC.configure PCconfig @10:01:17.19 PS.configure PSconfig @10:01:17.88 Forearm.configure LooseForearm @10:01:17.98 Elbow.configure LooseElbow @10:01:18.10 SC.configure SCloose @10:01:18.20 SE.configure SEconfig @10:01:18.25 CH.coast @10:01:18.30 CH.configure CHconfig @10:01:18.34 PH.coast @10:01:18.40 PH.configure PHconfig @10:01:18.44 PCR.off @10:01:18.47 PCR.pidtemp= 50,0.1,0,300,25,25 @10:01:18.50 PCR.caltemp= 2.8814,0.0372,0 @10:01:18.53 All dwarf controllers running firmware version 3.75 @10:01:18.64 Valve.reset @10:01:18.81 PCR.off @10:01:18.84 PCR.pidtemp= 50,0.1,0,300,25,25 @10:01:18.87 PCR.caltemp= 2.8814,0.0372,0 @10:01:19.10 PSV.dial :air @10:01:19.12 Intake.select :close @10:01:19.16 Exhaust.select :close @10:01:19.19 CTV.dial :air @10:01:19.28 CSV.dial :waste1 @10:01:19.43 SSV.dial :spit,avoiding: :exhaust @10:01:19.47 PS.reconfigure PSconfig @10:01:19.49
Forearm.reconfigure LooseForearm @10:01:19.63 PS.home.seek 0ml @10:01:19.65
Forearm.home.seek :retract @10:01:19.83 CS.reconfigure CSfast @10:01:19.87 SS.reconfigure SSconfig @10:01:19.96 CS.home.seek 0ml @10:01:20.00 SS.home.seek 0ml @10:01:20.43 Hand.close @10:01:20.46
Elbow.jog 1500 @10:01:21.08 Elbow.reconfigure LooseElbow @10:01:21.13 Elbow.home.seek :clear @10:01:25.87 SE.reconfigure SEconfig @10:01:25.92 SE.home.seek :down @10:01:26.55 SC.reconfigure SCsloppy @10:01:26.60 SC.home.seek :initial @10:01:28.73 PS.coast @10:01:30.63
Forearm.seek :retract @10:01:30.71 Elbow.seek :hammer @10:01:32.02 Forearm.reconfigure TightForearm @10:01:32.07 Forearm.seek 5 + 350 counts @10:01:33.09 Forearm.seek :retract @10:01:34.33 CC.reconfigure CCconfig @10:01:34.35 PC.reconfigure PCconfig @10:01:34.45
CC.home.seek :open @10:01:34.49 PC.home.seek :open @10:01:37.98 GoodNews.email "At 10:01:37 began in tube 2: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:124\n{1 => 15, 2 => 7}\nCan@10:01:37, 8.1C, 43% humidity, 14.3psia, 13.795V, 0.607A, 0.607A avg, 8.37W",Subject: "Started 20invasivedeployfinal phase stdphase" @10:01:38.01
Phase [stdphase] @10:01:38.04CEST18-May-20 ===> STARTED Superphase (phase #5) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @10:01:38.05 Starting initialPurge @10:01:38.07 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:01:38.14 Hand.open @10:01:38.97 Elbow.reconfigure TightElbow @10:01:39.02 Elbow.seek FlushPuck::Garage @10:01:41.09 Elbow.reconfigure LooseElbow @10:01:41.36 Forearm.reconfigure LooseForearm @10:01:41.47 Forearm.seek :garage @10:01:42.48 Hand.close @10:01:43.20 Forearm.seek :retract @10:01:44.26 Elbow.reconfigure TightElbow @10:01:44.31 Elbow.seek CC @10:01:47.38 Elbow.reconfigure LooseElbow @10:01:47.61 Forearm.reconfigure TightForearm @10:01:47.71 Forearm.seek CC @10:01:49.39 CC.seek :closed @10:01:53.84 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 1.94 @10:01:54.08 Hand.open @10:01:54.90 Forearm.reconfigure LooseForearm @10:01:54.98 Forearm.seek :retract @10:01:56.09 Flushing the Collection Valve::Manifold @10:01:56.38 CSV.dial CSR @10:01:57.91 Pulling 5ml of CSR.flush @10:01:57.95 CSR.select :flush @10:01:58.12 CS.reconfigure CSconfig @10:01:58.22 CS.seek 5ml @10:02:12.08 delay 5 seconds @10:02:17.13 CSR.select :air @10:02:17.38 CS.reconfigure CSfast @10:02:17.49 CS.seek 7.5ml @10:02:24.33 CSV.dial :waste @10:02:25.73 CS.reconfigure CSconfig @10:02:25.85 CS.seek 0ml @10:02:46.21 Purging external Intake @10:02:46.35 CSV.dial :flush @10:02:46.85 CS.reconfigure CSfast @10:02:46.90 CS.seek 10ml @10:03:10.27 delay 5 seconds @10:03:15.30 CSV.dial CBV @10:03:15.34
CBV.dial CSV @10:03:16.13 CTV.dial :intake @10:03:16.91 Intake.select :open @10:03:17.03 CS.seek 0ml @10:03:41.93 Intake.select :close @10:03:42.04 CTV.dial :air @10:03:42.82 Flushing upper manifold @10:03:42.95 CSV.dial :flush @10:03:43.72 CS.seek 5ml @10:03:56.43 delay 5 seconds @10:04:01.46 CSV.dial :air @10:04:02.00 CS.seek 7ml @10:04:07.49 CSV.dial CBV @10:04:07.53
CBV.dial CSV @10:04:08.59 CTV.dial :air @10:04:08.61 CS.seek 0ml @10:04:25.24 delay 7 seconds @10:04:32.28 CS.seek 3ml @10:04:40.05 CSV.dial :waste @10:04:40.53 CS.seek 0ml @10:04:48.43 CSV.dial :flush @10:04:48.91 CS.seek 3ml @10:04:56.74 delay 5 seconds @10:05:01.77 CSV.dial :air @10:05:02.30 CS.seek 5ml @10:05:07.84 CSV.dial :air @10:05:07.86 CS.seek 0ml @10:05:20.09 delay 7 seconds @10:05:27.13 CS.seek 3ml @10:05:34.93 CSV.dial :waste @10:05:35.69 CS.seek 0ml @10:05:43.43 Garaging FlushPuck in Collection Clamp @10:05:43.51 Elbow.reconfigure TightElbow @10:05:43.56 Elbow.seek CC @10:05:43.81 Elbow.reconfigure LooseElbow @10:05:43.92 Forearm.seek CC @10:05:44.91 Hand.close @10:05:45.58 CC.seek :open @10:05:49.91 Forearm.seek :retract @10:05:50.92 Elbow.reconfigure TightElbow @10:05:50.97 Elbow.seek :garage @10:05:54.23 Elbow.reconfigure LooseElbow @10:05:54.33 Forearm.reconfigure TightForearm @10:05:54.38 Forearm.seek :garage @10:05:55.51 Hand.open @10:05:56.15 Forearm.reconfigure LooseForearm @10:05:56.19 Forearm.seek :retract @10:05:57.28 Starting mfb @10:05:57.30 pcrBAC sampling at most 1500ml, wcr at most 1500ml @10:05:57.34 BACfans.email "Started mfb in tube 2 at 10:05:57CEST18-May-20\n{1 => 15, 2 => 7}\nCan@10:04:38, 8.1C, 43% humidity, 14.3psia, 13.761V, 0.708A, 0.708A avg, 9.74W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @10:05:57.41
Loading sh1 puck from tube 2 into Collection Clamp @10:05:57.54 Readying the MFB @10:05:57.60 Homing MFB Syringe @10:05:57.61 AFV.select :holdingCoil @10:05:57.88 ATV.dial :air2 @10:05:58.00
SC.reconfigure SCloose @10:05:58.27 Elbow.reconfigure TightElbow @10:05:58.32 Elbow.seek 2 @10:05:59.35 AS.reconfigure ASconfig @10:05:59.40 AS.home.seek :home @10:06:00.46
Forearm.reconfigure TightForearm @10:06:00.52 Forearm.seek 2 + 700 counts @10:06:01.87 SE.reconfigure SEpick @10:06:01.98 SE.seek beamBreak @10:06:04.57 SE.configure SEconfig @10:06:05.07 AFV.select :carrierSolution @10:06:05.16
Elbow.seek 2 @10:06:05.39 delay 2 seconds @10:06:05.78
Forearm.seek 2 + 300 counts @10:06:06.08 Forearm.reconfigure LooseForearm @10:06:06.18 Hand.close @10:06:06.90 SE.seek :down @10:06:07.38
Forearm.seek :retract @10:06:07.52 AS.seek 1ml @10:06:08.44
Elbow.seek CC @10:06:09.38 SE.coast @10:06:10.23
Elbow.reconfigure LooseElbow @10:06:10.49 Forearm.reconfigure TightForearm @10:06:10.61 Forearm.seek CC @10:06:12.40 CC.seek :closed @10:06:16.84 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.07 @10:06:16.87 Hand.open @10:06:17.63 Forearm.reconfigure LooseForearm @10:06:17.72 Forearm.seek :retract @10:06:18.83 SC.coast @10:06:18.91 collectSample 1500ml w/bubble point of 55psi @10:06:18.93 Priming 7ml @10:06:18.95 Intake.select :close @10:06:18.98 Exhaust.select :close @10:06:19.01 SS.hold @10:06:19.50 Calibrating pressure and temperature sensors @10:06:19.53 CTV.dial :air @10:06:19.56 SSV.dial :spit,avoiding: :exhaust @10:06:19.64 SS.reconfigure SSfast @10:06:19.74 SS.seek 5ml @10:06:22.63 SSV.dial CBV @10:06:22.71
CBV.dial SSV @10:06:24.22 delay 3.8 seconds @10:06:28.34 SSV.dial :spit @10:06:29.79 SS.seek 0ml @10:06:32.55 SSV.dial CBV @10:06:33.99 CTV.dial :intake @10:06:34.86 SSV.dial CBV @10:06:34.92
CBV.dial SSV @10:06:34.95 Allowing 40s for 7.0ml of remaining 7.0ml @10:06:34.97 Intake.select :open @10:06:37.29 Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:06:37.32 SS.reconfigure SSinhale.with maxSpeed: 150 @10:06:37.42 SS.seek 7ml @10:06:40.84 Vacuum too high -- waiting up to 40s @10:06:43.41 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:06:43.45 SS.reconfigure SSinhale.with maxSpeed: 135 @10:06:43.58 SS.seek 7ml @10:06:46.60 Vacuum too high -- waiting up to 40s @10:06:49.16 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:06:49.20 SS.reconfigure SSinhale.with maxSpeed: 122 @10:06:49.29 SS.seek 7ml @10:06:51.63 Stroke completed -- waiting for pressure to equalize ... @10:06:55.61 Intake.select :close @10:06:55.86 SSV.dial :exhaust @10:06:56.73 Exhaust.select :open @10:06:56.91 SS.reconfigure SSexhale @10:06:57.00 SS.seek 0ml @10:07:00.47 delay 1 second @10:07:00.67
Exhaust.select :close @10:07:00.83 Sampling 1500.0ml @10:07:00.86 Intake.select :close @10:07:00.88 Exhaust.select :close @10:07:00.91 SS.reconfigure SSconfig @10:07:01.02 SS.hold @10:07:01.48 CTV.dial :intake @10:07:01.52 SSV.dial CBV @10:07:01.61
CBV.dial SSV @10:07:01.70 AFV.select :holdingCoil @10:07:01.88 delay 1 second @10:07:02.46
Allowing 130s for 25.0ml of remaining 1500.0ml @10:07:02.52 Intake.select :open @10:07:02.92 ATV.dial :waste @10:07:03.99 AS.reconfigure ASnorm @10:07:04.13 AS.home.seek :empty @10:07:04.80
Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:07:04.84 SS.reconfigure SSinhale.with maxSpeed: 122 @10:07:04.93 SS.seek 25ml @10:07:08.69 Vacuum too high -- waiting up to 40s @10:07:10.98 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:07:11.00 SS.reconfigure SSinhale.with maxSpeed: 110 @10:07:11.05 SS.seek 25ml @10:07:13.84 Vacuum too high -- waiting up to 40s @10:07:15.90 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:07:15.92 SS.reconfigure SSinhale.with maxSpeed: 99 @10:07:15.96 SS.seek 25ml @10:07:18.41 Vacuum too high -- waiting up to 40s @10:07:20.33 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:07:20.35 SS.reconfigure SSinhale.with maxSpeed: 89 @10:07:20.40 SS.seek 25ml @10:07:22.61 Vacuum too high -- waiting up to 40s @10:07:24.48 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:07:24.50 SS.reconfigure SSinhale.with maxSpeed: 80 @10:07:24.54 SS.seek 25ml @10:07:27.14 Vacuum too high -- waiting up to 40s @10:07:28.98 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:07:29.00 SS.reconfigure SSinhale.with maxSpeed: 72 @10:07:29.05 SS.seek 25ml @10:07:31.71 Vacuum too high -- waiting up to 40s @10:07:33.52 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:07:33.54 SS.reconfigure SSinhale.with maxSpeed: 65 @10:07:33.59 SS.seek 25ml @10:07:36.27 Vacuum too high -- waiting up to 40s @10:07:37.84 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:07:37.85 SS.reconfigure SSinhale.with maxSpeed: 59 @10:07:37.90 SS.seek 25ml @10:07:40.96 Vacuum too high -- waiting up to 40s @10:07:42.79 SS.reconfigure SSinhale.with maxSpeed: 53 @10:07:42.84 SS.seek 25ml @10:07:46.49 Vacuum too high -- waiting up to 40s @10:07:48.05 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:07:48.07 SS.reconfigure SSinhale.with maxSpeed: 48 @10:07:48.12 SS.seek 25ml @10:07:52.52 Vacuum too high -- waiting up to 40s @10:07:54.20 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:07:54.21 SS.reconfigure SSinhale.with maxSpeed: 43 @10:07:54.26 SS.seek 25ml @10:07:56.12 Stroke completed -- waiting for pressure to equalize ... @10:07:56.79 ATV.dial :waste @10:07:56.81 ARV.dial between carrier and air1 @10:07:56.90 ASV.dial :waste1 @10:07:59.97
Intake.select :close @10:08:00.13 SSV.dial :exhaust @10:08:00.94 Exhaust.select :open @10:08:01.06 SS.reconfigure SSexhale @10:08:01.10 SS.seek 0ml @10:08:10.31 Exhaust.select :close @10:08:10.43 SSV.dial CBV @10:08:10.45
CBV.dial SSV @10:08:11.28 Allowing 130s for 25.0ml of remaining 1475.0ml @10:08:11.29 Intake.select :open @10:08:13.47 Intake @31.9psia, vacuum from 20.0 to 24.0psi @10:08:13.48 SS.reconfigure SSinhale.with maxSpeed: 43 @10:08:13.53 SS.seek 25ml @10:08:21.14 Vacuum too high -- waiting up to 40s @10:08:22.45 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:08:22.47 SS.reconfigure SSinhale.with maxSpeed: 39 @10:08:22.52 SS.seek 25ml @10:08:31.27 Vacuum too high -- waiting up to 40s @10:08:32.59 SS.reconfigure SSinhale.with maxSpeed: 35 @10:08:32.64 SS.seek 25ml @10:08:48.21 Vacuum too high -- waiting up to 40s @10:08:49.78 SS.reconfigure SSinhale.with maxSpeed: 32 @10:08:49.83 SS.seek 25ml @10:09:08.71 Stroke completed -- waiting for pressure to equalize ... @10:09:13.81 Intake.select :close @10:09:13.97 SSV.dial :exhaust @10:09:14.78 Exhaust.select :open @10:09:14.90 SS.reconfigure SSexhale @10:09:14.94 SS.seek 0ml @10:09:24.49 Exhaust.select :close @10:09:24.61 SSV.dial CBV @10:09:24.63
CBV.dial SSV @10:09:25.45 Allowing 130s for 25.0ml of remaining 1450.0ml @10:09:25.46 Intake.select :open @10:09:27.64 Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:09:27.65 SS.reconfigure SSinhale.with maxSpeed: 32 @10:09:27.70 SS.seek 25ml @10:09:52.80 Vacuum too high -- waiting up to 40s @10:09:54.14 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:09:54.15 SS.reconfigure SSinhale.with maxSpeed: 29 @10:09:54.20 SS.seek 25ml @10:10:27.05 Stroke completed -- waiting for pressure to equalize ... @10:10:32.11 Intake.select :close @10:10:32.27 SSV.dial :exhaust @10:10:33.06 Exhaust.select :open @10:10:33.18 SS.reconfigure SSexhale @10:10:33.23 SS.seek 0ml @10:10:42.26 Exhaust.select :close @10:10:42.38 SSV.dial CBV @10:10:42.40
CBV.dial SSV @10:10:43.22 Allowing 130s for 25.0ml of remaining 1425.0ml @10:10:43.23 Intake.select :open @10:10:45.41 Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:10:45.42 SS.reconfigure SSinhale.with maxSpeed: 29 @10:10:45.47 SS.seek 25ml @10:11:33.96 Vacuum too high -- waiting up to 40s @10:11:35.52 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:11:35.54 SS.reconfigure SSinhale.with maxSpeed: 26 @10:11:35.59 SS.seek 25ml @10:11:49.53 Stroke completed -- waiting for pressure to equalize ... @10:11:54.58 Intake.select :close @10:11:54.74 SSV.dial :exhaust @10:11:55.55 Exhaust.select :open @10:11:55.67 SS.reconfigure SSexhale @10:11:55.72 SS.seek 0ml @10:12:05.12 Exhaust.select :close @10:12:05.24 SSV.dial CBV @10:12:05.26
CBV.dial SSV @10:12:06.09 Allowing 130s for 25.0ml of remaining 1400.0ml @10:12:06.10 Intake.select :open @10:12:08.27 Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:12:08.29 SS.reconfigure SSinhale.with maxSpeed: 26 @10:12:08.34 SS.seek 25ml @10:13:15.83 Stroke completed -- waiting for pressure to equalize ... @10:13:21.49 Intake.select :close @10:13:21.65 SSV.dial :exhaust @10:13:22.45 Exhaust.select :open @10:13:22.57 SS.reconfigure SSexhale @10:13:22.62 SS.seek 0ml @10:13:32.03 Exhaust.select :close @10:13:32.15 SSV.dial CBV @10:13:32.17
CBV.dial SSV @10:13:32.98 Allowing 130s for 25.0ml of remaining 1375.0ml @10:13:32.99 Intake.select :open @10:13:35.17 Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:13:35.18 SS.reconfigure SSinhale.with maxSpeed: 26 @10:13:35.23 SS.seek 25ml @10:14:42.58 Stroke completed -- waiting for pressure to equalize ... @10:14:48.35 Intake.select :close @10:14:48.52 SSV.dial :exhaust @10:14:49.34 Exhaust.select :open @10:14:49.46 SS.reconfigure SSexhale @10:14:49.51 SS.seek 0ml @10:14:58.34 Exhaust.select :close @10:14:58.46 SSV.dial CBV @10:14:58.48
CBV.dial SSV @10:14:59.31 Allowing 130s for 25.0ml of remaining 1350.0ml @10:14:59.32 Intake.select :open @10:15:01.49 Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:15:01.51 SS.reconfigure SSinhale.with maxSpeed: 26 @10:15:01.55 SS.seek 25ml @10:16:09.18 Stroke completed -- waiting for pressure to equalize ... @10:16:15.48 Intake.select :close @10:16:15.64 SSV.dial :exhaust @10:16:16.44 Exhaust.select :open @10:16:16.56 SS.reconfigure SSexhale @10:16:16.60 SS.seek 0ml @10:16:25.69 Exhaust.select :close @10:16:25.81 SSV.dial CBV @10:16:25.83
CBV.dial SSV @10:16:26.64 Allowing 130s for 25.0ml of remaining 1325.0ml @10:16:26.65 Intake.select :open @10:16:28.82 Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:16:28.84 SS.reconfigure SSinhale.with maxSpeed: 26 @10:16:28.89 SS.seek 25ml @10:17:23.27 Vacuum too high -- waiting up to 40s @10:17:25.30 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:17:25.42 SS.reconfigure SSinhale.with maxSpeed: 23 @10:17:25.46 SS.seek 25ml @10:17:40.27 Stroke completed -- waiting for pressure to equalize ... @10:17:46.02 Intake.select :close @10:17:46.18 SSV.dial :exhaust @10:17:46.97 Exhaust.select :open @10:17:47.09 SS.reconfigure SSexhale @10:17:47.13 SS.seek 0ml @10:17:56.19 Exhaust.select :close @10:17:56.31 SSV.dial CBV @10:17:56.33
CBV.dial SSV @10:17:57.16 Allowing 130s for 25.0ml of remaining 1300.0ml @10:17:57.17 Intake.select :open @10:17:59.36 Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:17:59.38 SS.reconfigure SSinhale.with maxSpeed: 23 @10:17:59.42 SS.seek 25ml @10:19:15.49 Stroke completed -- waiting for pressure to equalize ... @10:19:21.81 Intake.select :close @10:19:21.97 SSV.dial :exhaust @10:19:22.77 Exhaust.select :open @10:19:22.89 SS.reconfigure SSexhale @10:19:22.94 SS.seek 0ml @10:19:32.05 Exhaust.select :close @10:19:32.17 SSV.dial CBV @10:19:32.19
CBV.dial SSV @10:19:33.02 Allowing 130s for 25.0ml of remaining 1275.0ml @10:19:33.03 Intake.select :open @10:19:35.20 Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:19:35.22 SS.reconfigure SSinhale.with maxSpeed: 23 @10:19:35.27 SS.seek 25ml @10:20:51.32 Stroke completed -- waiting for pressure to equalize ... @10:20:58.86 Intake.select :close @10:20:59.02 SSV.dial :exhaust @10:20:59.81 Exhaust.select :open @10:20:59.93 SS.reconfigure SSexhale @10:20:59.98 SS.seek 0ml @10:21:09.03 Exhaust.select :close @10:21:09.15 SSV.dial CBV @10:21:09.17
CBV.dial SSV @10:21:09.99 Allowing 130s for 25.0ml of remaining 1250.0ml @10:21:10.00 Intake.select :open @10:21:12.74 Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:21:12.75 SS.reconfigure SSinhale.with maxSpeed: 23 @10:21:12.81 SS.seek 25ml @10:22:04.74 Vacuum too high -- waiting up to 40s @10:22:06.66 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:22:06.68 SS.reconfigure SSinhale.with maxSpeed: 21 @10:22:06.73 SS.seek 25ml @10:22:33.46 Stroke completed -- waiting for pressure to equalize ... @10:22:41.02 Intake.select :close @10:22:41.18 SSV.dial :exhaust @10:22:41.99 Exhaust.select :open @10:22:42.11 SS.reconfigure SSexhale @10:22:42.15 SS.seek 0ml @10:22:51.11 Exhaust.select :close @10:22:51.23 SSV.dial CBV @10:22:51.25
CBV.dial SSV @10:22:52.06 Allowing 130s for 25.0ml of remaining 1225.0ml @10:22:52.07 Intake.select :open @10:22:54.25 Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:22:54.26 SS.reconfigure SSinhale.with maxSpeed: 21 @10:22:54.31 SS.seek 25ml @10:24:17.99 Stroke completed -- waiting for pressure to equalize ... @10:24:26.78 Intake.select :close @10:24:26.94 SSV.dial :exhaust @10:24:27.72 Exhaust.select :open @10:24:27.84 SS.reconfigure SSexhale @10:24:27.88 SS.seek 0ml @10:24:37.05 Exhaust.select :close @10:24:37.17 SSV.dial CBV @10:24:37.19
CBV.dial SSV @10:24:38.02 Allowing 130s for 25.0ml of remaining 1200.0ml @10:24:38.03 Intake.select :open @10:24:40.21 Intake @31.9psia, vacuum from 20.0 to 24.0psi @10:24:40.23 SS.reconfigure SSinhale.with maxSpeed: 21 @10:24:40.27 SS.seek 25ml @10:25:25.84 Vacuum too high -- waiting up to 40s @10:25:27.98 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:25:28.00 SS.reconfigure SSinhale.with maxSpeed: 19 @10:25:28.05 SS.seek 25ml @10:26:10.24 Stroke completed -- waiting for pressure to equalize ... @10:26:19.11 Intake.select :close @10:26:19.27 SSV.dial :exhaust @10:26:20.08 Exhaust.select :open @10:26:20.20 SS.reconfigure SSexhale @10:26:20.25 SS.seek 0ml @10:26:29.39 Exhaust.select :close @10:26:29.51 SSV.dial CBV @10:26:29.53
CBV.dial SSV @10:26:30.35 Allowing 130s for 25.0ml of remaining 1175.0ml @10:26:30.36 Intake.select :open @10:26:32.53 Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:26:32.55 SS.reconfigure SSinhale.with maxSpeed: 19 @10:26:32.60 SS.seek 25ml @10:27:44.40 Vacuum too high -- waiting up to 40s @10:27:46.71 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:27:46.73 SS.reconfigure SSinhale.with maxSpeed: 17 @10:27:46.77 SS.seek 25ml @10:28:09.92 Stroke completed -- waiting for pressure to equalize ... @10:28:18.69 Intake.select :close @10:28:18.85 SSV.dial :exhaust @10:28:19.64 Exhaust.select :open @10:28:20.23 SS.reconfigure SSexhale @10:28:20.38 SS.seek 0ml @10:28:29.71 Exhaust.select :close @10:28:29.83 SSV.dial CBV @10:28:29.85
CBV.dial SSV @10:28:30.69 Allowing 130s for 25.0ml of remaining 1150.0ml @10:28:30.70 Intake.select :open @10:28:32.87 Intake @31.9psia, vacuum from 20.0 to 24.0psi @10:28:32.89 SS.reconfigure SSinhale.with maxSpeed: 17 @10:28:32.94 SS.seek 25ml @10:30:07.59 Vacuum too high -- waiting up to 40s @10:30:10.18 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:30:10.19 SS.reconfigure SSinhale.with maxSpeed: 15 @10:30:10.24 SS.seek 25ml @10:30:20.01 Stroke completed -- waiting for pressure to equalize ... @10:30:29.44 Intake.select :close @10:30:29.60 SSV.dial :exhaust @10:30:30.41 Exhaust.select :open @10:30:30.53 SS.reconfigure SSexhale @10:30:30.58 SS.seek 0ml @10:30:39.60 Exhaust.select :close @10:30:39.72 SSV.dial CBV @10:30:39.74
CBV.dial SSV @10:30:40.55 Allowing 130s for 25.0ml of remaining 1125.0ml @10:30:40.56 Intake.select :open @10:30:42.73 Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:30:42.75 SS.reconfigure SSinhale.with maxSpeed: 15 @10:30:42.80 SS.seek 25ml @10:32:39.96 Stroke completed -- waiting for pressure to equalize ... @10:32:50.57 SS.reconfigure SSconfig @10:32:50.63 SS.hold @10:32:50.76 Intake.select :close @10:32:50.91 ShallowSampler::Clogged in MAIN -- progress too slow after 400.0ml @10:32:50.92 SSV.dial :exhaust @10:32:51.71 Exhaust.select :open @10:32:51.83 SS.reconfigure SSexhale @10:32:51.87 SS.seek 0ml @10:33:01.26 Exhaust.select :close @10:33:01.37 Sampled 400.0ml @10:33:01.39 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:33:01.40 Intake.select :close @10:33:01.47 CSV.dial :waste @10:33:01.55 CS.seek 0ml @10:33:01.63 SSV.dial CBV,avoiding: :exhaust @10:33:01.66 CSV.dial :air @10:33:01.70
CBV.dial SSV @10:33:02.52 CS.seek 10ml @10:33:03.69
CTV.dial :air @10:33:04.48 delay 15 seconds @10:33:19.51 SSV.dial :spit,avoiding: :exhaust @10:33:20.91 CTV.dial CSV,avoiding: :intake @10:33:20.96
SS.seek 0ml @10:33:21.09 SSV.dial CBV,avoiding: :exhaust @10:33:26.56 CBV.dial between SSV and waste2 @10:33:27.00 CTV.dial CSV,avoiding: :air @10:33:27.02
CSV.dial CTV @10:33:27.51 CS.reconfigure CSpressurize @10:33:27.55 CS.seek 0ml @10:33:53.34 BubblePoint: Intake @69.7psia, Outlet @14.0psia @10:33:53.35 CBV.dial SSV @10:33:53.70 SS.reconfigure SSconfig @10:33:53.76 SS.seek 10ml @10:33:59.32 delay 15 seconds @10:34:14.47 Evacuated: Intake @36.1psia, Outlet @0.4psia @10:34:14.48 SSV.dial :spit,avoiding: :exhaust @10:34:15.87 SS.reconfigure SSexhale @10:34:15.92 SS.seek 0ml @10:34:21.27 SS.reconfigure SSfast @10:34:21.32 SS.seek 5ml @10:34:24.10 SSV.dial CBV,avoiding: :exhaust @10:34:24.14
CBV.dial SSV @10:34:25.52 CTV.dial :air,avoiding: :intake @10:34:26.90 CSV.dial CTV @10:34:26.95
SS.seek 10ml @10:34:27.95 CSV.dial :air @10:34:28.42 CS.reconfigure CSfast @10:34:28.47 CS.seek 0ml @10:34:29.55
SSV.dial :spit,avoiding: :exhaust @10:34:29.75 CS.coast @10:34:30.93
SS.reconfigure SSexhale @10:34:30.98 SS.seek 0ml @10:34:36.23 SS.reconfigure SSfast @10:34:36.28 SS.seek 5ml @10:34:38.80 SSV.dial CBV,avoiding: :exhaust @10:34:38.84
CBV.dial SSV @10:34:41.23 SSV.dial :spit,avoiding: :exhaust @10:34:42.63 SS.reconfigure SSconfig @10:34:42.69 SS.seek 0ml @10:34:45.82 SS.coast @10:34:45.86 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:34:45.87 Intake.select :close @10:34:45.95 CSV.dial :waste @10:34:46.09 SSV.dial CBV,avoiding: :exhaust @10:34:46.67
CBV.dial SSV @10:34:46.77 CS.seek 0ml @10:34:46.83 CSV.dial :air @10:34:47.60 CS.seek 10ml @10:34:48.07
CTV.dial :air @10:34:48.09 delay 15 seconds @10:35:03.11 SSV.dial :spit,avoiding: :exhaust @10:35:04.50 CTV.dial CSV,avoiding: :intake @10:35:04.58
SS.reconfigure SSexhale @10:35:04.63 SS.seek 0ml @10:35:04.71 SSV.dial CBV,avoiding: :exhaust @10:35:11.23 CBV.dial between SSV and waste2 @10:35:11.72 CSV.dial between air and CTV @10:35:12.06 CS.seek 8.252ml @10:35:17.12 CTV.dial CSV,avoiding: :air @10:35:17.15
CSV.dial CTV @10:35:17.49 CS.reconfigure CSpressurize @10:35:17.53 CS.seek 0ml @10:35:39.31 CSV.dial :air @10:35:39.79 CS.reconfigure CSfast @10:35:39.83 CS.seek 10ml @10:36:03.31 CSV.dial between air and CTV @10:36:03.67 CS.seek 3.046ml @10:36:20.32 CTV.dial CSV,avoiding: :air @10:36:20.34
CSV.dial CTV @10:36:20.67 CS.reconfigure CSpressurize @10:36:20.71 CS.seek 0ml @10:36:27.93 BubblePoint: Intake @68.8psia, Outlet @13.7psia @10:36:27.94 CBV.dial SSV @10:36:28.28 SS.reconfigure SSconfig @10:36:28.33 SS.seek 10ml @10:36:33.73 delay 15 seconds @10:36:48.87 Evacuated: Intake @39.7psia, Outlet @14.5psia @10:36:48.88 SSV.dial :spit,avoiding: :exhaust @10:36:50.27 SS.reconfigure SSexhale @10:36:50.32 SS.seek 0ml @10:36:55.25 SS.reconfigure SSfast @10:36:55.30 SS.seek 5ml @10:36:58.23 SSV.dial CBV,avoiding: :exhaust @10:36:58.26
CBV.dial SSV @10:36:59.65 CTV.dial :air,avoiding: :intake @10:37:01.03 CSV.dial CTV @10:37:01.08
SS.seek 10ml @10:37:02.08 CSV.dial :air @10:37:02.55 CS.reconfigure CSfast @10:37:02.60 CS.seek 0ml @10:37:03.71
SSV.dial :spit,avoiding: :exhaust @10:37:04.75 CS.coast @10:37:05.10
SS.reconfigure SSexhale @10:37:05.16 SS.seek 0ml @10:37:10.08 SS.reconfigure SSfast @10:37:10.13 SS.seek 5ml @10:37:12.77 SSV.dial CBV,avoiding: :exhaust @10:37:12.80
CBV.dial SSV @10:37:15.19 SSV.dial :spit,avoiding: :exhaust @10:37:16.58 SS.reconfigure SSconfig @10:37:16.63 SS.seek 0ml @10:37:20.33 SS.coast @10:37:20.38 Lysing: Adding 1.5ml lysis @10:37:20.39 CBV.dial between CSV and waste1,avoiding: :DBV @10:37:21.94 CTV.dial CSV @10:37:23.33 CSV.dial CSR @10:37:24.16 Pulling 1ml of CSR.lysis @10:37:24.17 CSR.select :lysis @10:37:24.29 CS.reconfigure CSconfig @10:37:24.33 CS.seek 1ml @10:37:27.94 delay 5 seconds @10:37:32.97 CSR.select :air @10:37:33.15 CS.seek 6ml @10:37:46.97 delay 2 seconds @10:37:48.99 CSV.dial :waste1 @10:37:50.33 CS.reconfigure CSfast @10:37:50.38 CS.seek :empty @10:38:04.78 CSV.dial CSR @10:38:06.17 Pulling 1.5ml of CSR.lysis @10:38:06.18 CSR.select :lysis @10:38:06.30 CS.reconfigure CSconfig @10:38:06.34 CS.seek 1.5ml @10:38:11.16 delay 5 seconds @10:38:16.19 CSR.select :air @10:38:16.36 CS.seek 4.5ml @10:38:25.00 delay 2 seconds @10:38:27.02 CSV.dial CTV @10:38:27.51 CS.seek 1.275ml @10:38:36.86 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @10:38:36.88 CH.seek 85C,8:00 @10:41:43.25 CH.hold 5:00,85C @10:46:43.42 Collection Puck Heater at 85C after lysing @10:46:44.03 CS.seek 0ml @10:46:48.48 CTV.dial between spare and air,avoiding: :intake @10:46:49.58 CSV.dial :air @10:46:50.06 CS.seek 5ml @10:47:03.80 CSV.dial CTV @10:47:04.28 delay 2 seconds @10:47:06.30 CSV.dial :air @10:47:06.80 delay 2 seconds @10:47:08.82 CSV.dial CTV @10:47:09.31 delay 2 seconds @10:47:11.33 CSV.dial :air @10:47:11.81 delay 2 seconds @10:47:13.84 CS.seek 0ml @10:47:27.61 Recovering Lysate into Collection syringe @10:47:27.62 CSV.dial CBV @10:47:28.67 CBV.dial CSV @10:47:29.07 CS.seek 5ml @10:47:42.76 delay 5 seconds @10:47:47.79 CTV.dial :air @10:47:48.34 CS.seek 6.999ml @10:47:54.48 delay 5 seconds @10:47:59.51 CSV.dial :air2 @10:47:59.99 CS.seek 10ml @10:48:08.61 CSV.dial between air and CTV,avoiding: CBV @10:48:09.80 Lysing Complete @10:48:09.88 Storing sh1 puck in Collection Clamp to tube 1 @10:48:10.07 SC.reconfigure SCtight @10:48:10.12 SC.seek 1 @10:48:12.27 SC.reconfigure SCloose @10:48:12.49 Elbow.reconfigure TightElbow @10:48:12.54 Elbow.seek 1 @10:48:14.46 Forearm.reconfigure TightForearm @10:48:14.52 Forearm.seek 1 + 700 counts @10:48:15.58 SE.reconfigure SEpick @10:48:15.67 SE.seek beamBreak @10:48:17.42 SE.configure SEconfig @10:48:17.58 Elbow.reconfigure LimpElbow @10:48:17.64 Forearm.reconfigure LooseForearm @10:48:17.69 Forearm.seek 1 @10:48:17.98 Elbow.hold @10:48:18.06 Elbow.reconfigure TightElbow @10:48:18.23 SE.seek 14.1 Pucks below Top Plate @10:48:18.72 Forearm.force -20 @10:48:18.76 Forearm.seek 1 + 346 counts @10:48:19.09 Forearm.reconfigure TightForearm @10:48:19.14 Forearm.seek 1 + 700 counts @10:48:19.49 SE.seek 15.1 Pucks below Top Plate @10:48:20.10 Forearm.reconfigure LooseForearm @10:48:20.15 Forearm.seek :retract @10:48:21.07 Elbow.seek CC @10:48:22.79 Elbow.reconfigure LooseElbow @10:48:23.46 Forearm.seek CC @10:48:24.51 Hand.close @10:48:25.15 CC.seek :open @10:48:29.65 Forearm.seek :retract @10:48:30.68 Elbow.reconfigure TightElbow @10:48:30.73 Elbow.seek 1 @10:48:32.64 Elbow.reconfigure LimpElbow @10:48:32.69 Forearm.seek 1 @10:48:33.72 Elbow.hold @10:48:33.80 Elbow.reconfigure TightElbow @10:48:33.85 Hand.open @10:48:34.67 Forearm.force -20 @10:48:34.71 Forearm.seek 1 + 350 counts @10:48:35.03 Forearm.reconfigure TightForearm @10:48:35.07 Forearm.seek 1 + 700 counts @10:48:35.45 SE.seek :down @10:48:36.61
Forearm.reconfigure LooseForearm @10:48:36.66 Forearm.seek :retract @10:48:36.73 SE.coast @10:48:37.62
SC.coast @10:48:37.66 Tranferring lysate to PRV @10:48:37.67 CSV.dial :air @10:48:38.01 CS.seek 10ml @10:48:38.08 CSV.dial between air and CTV @10:48:38.42 AIV.dial :bypass @10:48:38.62 Beginning samplePassC @10:48:38.63 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:48:38.71 Hand.open @10:48:39.04 Elbow.seek FlushPuck::Garage @10:48:41.04 Elbow.reconfigure LooseElbow @10:48:41.15 Forearm.seek :garage @10:48:42.17 Hand.close @10:48:42.91 Forearm.seek :retract @10:48:43.93 Elbow.reconfigure TightElbow @10:48:43.98 Elbow.seek CC @10:48:46.89 Elbow.reconfigure LooseElbow @10:48:46.99 Forearm.reconfigure TightForearm @10:48:47.04 Forearm.seek CC @10:48:48.36 CC.seek :closed @10:48:53.29 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.94 @10:48:53.38 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[66:27->20] @10:48:53.40 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[66:27->20] @10:48:53.43 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[66:27->20] @10:48:53.46 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[66:27->20] @10:48:53.48 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[66:27->20] @10:48:53.51 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[66:27->20] @10:48:53.54 Hand.open @10:48:54.22 Forearm.reconfigure LooseForearm @10:48:54.27 Forearm.seek :retract @10:48:55.29 AWV.select :air @10:48:55.30 AIV.dial :bypass @10:48:55.31 PSV.dial PSR @10:48:56.71 Pulling 1.5ml of PSR.flush @10:48:56.72 PSR.select :flush @10:48:56.86 PS.seek 1.5ml @10:49:12.90 delay 5 seconds @10:49:17.93 PSV.dial :waste1 @10:49:18.40 PS.seek 0ml @10:49:34.47 PSV.dial PSR @10:49:34.95 PSR.select :air @10:49:35.07 PS.seek 2.5ml @10:50:01.23 PRV.dial CBV @10:50:01.25
CBV.dial PRV @10:50:02.11 PSV.dial :mixloop @10:50:03.15 CTV.dial :air,avoiding: :intake @10:50:03.16 PTV.dial :PRVmixing @10:50:03.26 PS.seek 0ml @10:50:29.40 PSV.dial :air @10:50:29.88 PS.seek 2.5ml @10:50:56.00 PSV.dial :mixloop @10:50:56.49 PS.seek 0ml @10:51:22.59 PSV.dial :air @10:51:23.05 PS.seek 2.5ml @10:51:49.12 PSV.dial :mixloop @10:51:49.59 PS.seek 0ml @10:52:15.78 Garaging FlushPuck in Collection Clamp @10:52:15.86 Elbow.reconfigure TightElbow @10:52:15.91 Elbow.seek CC @10:52:16.17 Elbow.reconfigure LooseElbow @10:52:16.28 Forearm.seek CC @10:52:17.32 Hand.close @10:52:17.91 CC.seek :open @10:52:22.28 Forearm.seek :retract @10:52:23.33 Elbow.reconfigure TightElbow @10:52:23.38 Elbow.seek :garage @10:52:26.58 Elbow.reconfigure LooseElbow @10:52:26.68 Forearm.reconfigure TightForearm @10:52:26.73 Forearm.seek :garage @10:52:27.91 Hand.open @10:52:28.61 Forearm.reconfigure LooseForearm @10:52:28.66 Forearm.seek :retract @10:52:29.69 Connecting PS to CS to transfer sample @10:52:29.70 CBV.dial AIV @10:52:29.71 AIV.dial :bypass @10:52:29.72 PSV.dial :mixloop @10:52:29.74 PTV.dial :PRVmixing @10:52:29.75 PRV.dial AIV @10:52:29.76 CTV.dial :air,avoiding: :intake @10:52:29.78 Loading lyfil puck from tube 2 into Collection Clamp @10:52:30.03 SC.reconfigure SCtight @10:52:30.08 SC.seek 2 @10:52:32.38 SC.reconfigure SCloose @10:52:33.08 Elbow.reconfigure TightElbow @10:52:33.22 Elbow.seek 2 @10:52:35.25 Forearm.reconfigure TightForearm @10:52:35.30 Forearm.seek 2 + 700 counts @10:52:36.34 SE.reconfigure SEpick @10:52:36.42 SE.seek beamBreak @10:52:39.12 SE.configure SEconfig @10:52:39.28 Elbow.seek 2 @10:52:39.52 Forearm.seek 2 + 300 counts @10:52:39.72 Forearm.reconfigure LooseForearm @10:52:39.76 Hand.close @10:52:40.43 SE.seek :down @10:52:40.66
Forearm.seek :retract @10:52:41.63 Elbow.seek CC @10:52:42.79 SE.coast @10:52:43.31
Elbow.reconfigure LooseElbow @10:52:43.41 Forearm.reconfigure TightForearm @10:52:43.46 Forearm.seek CC @10:52:44.85 CC.seek :closed @10:52:49.11 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.98 @10:52:49.12 Hand.open @10:52:49.87 Forearm.reconfigure LooseForearm @10:52:49.92 Forearm.seek :retract @10:52:50.94 SC.coast @10:52:50.98 CTV.dial CSV,avoiding: :intake @10:52:52.36 CSV.dial CTV @10:52:52.69 CS.seek 5ml @10:53:06.37 delay 1:20 @10:54:26.41 CS.seek 2.5ml @10:54:33.77 delay 40 seconds @10:55:13.81 CS.seek 0ml @10:55:21.40 delay 1:00 @10:56:21.43 PSV.dial :air @10:56:21.90 PS.seek 2.5ml @10:56:48.07 PSV.dial :mixloop @10:56:48.54 delay 3 seconds @10:56:51.56 PSV.dial :air @10:56:52.02 delay 2 seconds @10:56:54.04 PSV.dial :mixloop @10:56:54.50 delay 3 seconds @10:56:57.52 PSV.dial :waste1 @10:56:58.83 PS.seek :empty @10:57:25.04 CBV.dial :waste1 @10:57:25.53 delay 5 seconds @10:57:30.56 CBV.dial AIV @10:57:31.04 delay 10 seconds @10:57:41.07 CBV.dial :waste1 @10:57:41.56 delay 10 seconds @10:57:51.59 CBV.dial AIV @10:57:52.07 delay 10 seconds @10:58:02.10 CTV.dial CSV,avoiding: :intake @10:58:02.11 CBV.dial between AIV and waste1 @10:58:02.46 CSV.dial :air @10:58:02.94 CS.seek 10ml @10:58:29.53 CSV.dial CTV @10:58:30.01 CS.seek 0ml @10:58:56.68 CBV.dial :waste1 @10:58:57.03 delay 10 seconds @10:59:07.55 CTV.dial :air,avoiding: :intake @10:59:09.07 Storing lyfil puck in Collection Clamp to tube 1 @10:59:09.26 SC.reconfigure SCtight @10:59:09.31 SC.seek 1 @10:59:11.42 SC.reconfigure SCloose @10:59:11.65 Elbow.reconfigure TightElbow @10:59:11.70 Elbow.seek 1 @10:59:13.60 Forearm.reconfigure TightForearm @10:59:13.65 Forearm.seek 1 + 700 counts @10:59:14.68 SE.reconfigure SEpick @10:59:14.76 SE.seek beamBreak @10:59:16.45 SE.configure SEconfig @10:59:16.61 Elbow.reconfigure LimpElbow @10:59:16.67 Forearm.reconfigure LooseForearm @10:59:16.71 Forearm.seek 1 @10:59:16.98 Elbow.hold @10:59:17.05 Elbow.reconfigure TightElbow @10:59:17.16 SE.seek 15.11 Pucks below Top Plate @10:59:17.64 Forearm.force -20 @10:59:17.68 Forearm.seek 1 + 349 counts @10:59:18.00 Forearm.reconfigure TightForearm @10:59:18.05 Forearm.seek 1 + 700 counts @10:59:18.32 SE.seek 16.11 Pucks below Top Plate @10:59:19.01 Forearm.reconfigure LooseForearm @10:59:19.06 Forearm.seek :retract @10:59:20.00 Elbow.seek CC @10:59:21.64 Elbow.reconfigure LooseElbow @10:59:21.75 Forearm.seek CC @10:59:22.80 Hand.close @10:59:23.42 CC.seek :open @10:59:27.90 Forearm.seek :retract @10:59:28.94 Elbow.reconfigure TightElbow @10:59:28.99 Elbow.seek 1 @10:59:30.90 Elbow.reconfigure LimpElbow @10:59:30.95 Forearm.seek 1 @10:59:31.95 Elbow.hold @10:59:32.02 Elbow.reconfigure TightElbow @10:59:32.07 Hand.open @10:59:32.81 Forearm.force -20 @10:59:32.85 Forearm.seek 1 + 348 counts @10:59:33.16 Forearm.reconfigure TightForearm @10:59:33.21 Forearm.seek 1 + 700 counts @10:59:33.55 SE.seek :down @10:59:34.69
Forearm.reconfigure LooseForearm @10:59:34.76 Forearm.seek :retract @10:59:34.78 SE.coast @10:59:35.70
SC.coast @10:59:35.74 PSV.dial :air @10:59:37.41 PS.seek 0.499ml @10:59:43.35 delay 3 seconds @10:59:46.37 PSV.dial :mixloop @10:59:46.82 PRV.dial :air @10:59:47.32 delay 3 seconds @10:59:50.34 PRV.dial CBV @10:59:50.82 CBV.dial AIV @10:59:51.31 PRV.dial between CBV and air @10:59:51.65 PRV.dial :air @10:59:52.05 PS.reconfigure PSslow @10:59:52.10 PS.seek 0.599ml @10:59:54.66 PRV.dial :waste2 @10:59:55.41 PS.reconfigure PSconfig @10:59:55.46 PS.seek :empty @11:00:02.44 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:00:02.51 Hand.open @11:00:02.93 Elbow.seek FlushPuck::Garage @11:00:05.00 Elbow.reconfigure LooseElbow @11:00:05.11 Forearm.seek :garage @11:00:06.10 Hand.close @11:00:06.80 Forearm.seek :retract @11:00:07.84 Elbow.reconfigure TightElbow @11:00:07.89 Elbow.seek CC @11:00:10.83 Elbow.reconfigure LooseElbow @11:00:10.94 Forearm.reconfigure TightForearm @11:00:10.99 Forearm.seek CC @11:00:12.34 CC.seek :closed @11:00:16.59 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 1.94 @11:00:16.66 Hand.open @11:00:17.37 Forearm.reconfigure LooseForearm @11:00:17.42 Forearm.seek :retract @11:00:18.49 Clearing PML for modified lysate @11:00:18.50 CTV.dial :air,avoiding: :intake @11:00:18.51 PRV.dial :waste2 @11:00:18.52 PTV.dial :PRV,avoiding: :puck @11:00:19.30 delay 2 seconds @11:00:21.32 PTV.dial :PRVmixing,avoiding: :puck @11:00:22.10 delay 2 seconds @11:00:24.12 PSV.dial :PML @11:00:24.14 delay 2 seconds @11:00:26.16 PRV.dial :air @11:00:26.93 delay 2 seconds @11:00:29.01 PS.seek 1.499ml @11:00:45.01 delay 5 seconds @11:00:50.04 PS.seek 2.5ml @11:01:00.98 PSV.dial :air @11:01:01.46 PS.seek 0ml @11:01:27.57 mixing lysate with speDil @11:01:27.58 PSV.dial :PML @11:01:28.04 CBV.dial :PRV @11:01:28.05 PRV.dial :air @11:01:28.07 delay 2 seconds @11:01:30.09 PRV.dial :speDil @11:01:30.57 delay 2 seconds @11:01:33.23 PS.reconfigure PSslow1 @11:01:33.28 PS.seek 0.225ml @11:01:56.57 delay 5 seconds @11:02:01.60 PRV.dial CBV @11:02:02.38 delay 2 seconds @11:02:04.46 PS.seek 0.475ml @11:02:30.17 delay 5 seconds @11:02:35.20 PRV.dial :air @11:02:35.70 delay 2 seconds @11:02:37.73 PS.seek 0.965ml @11:03:27.70 delay 10 seconds @11:03:37.73 PRV.dial CBV @11:03:38.21 CTV.dial :air,avoiding: :intake @11:03:38.22 PSV.dial :air @11:03:38.68 PS.reconfigure PSconfig @11:03:38.74 PS.seek 2.5ml @11:03:55.04 delay 3 seconds @11:03:58.06 PSV.dial PTV @11:03:58.84 PTV.dial :PRV,avoiding: :puck @11:03:59.61 PS.seek 0ml @11:04:25.79 PSV.dial :air @11:04:26.55 PS.seek 2.5ml @11:04:52.67 delay 3 seconds @11:04:55.69 PSV.dial PTV @11:04:56.45 PTV.dial :PRV,avoiding: :puck @11:04:56.47 PS.seek 0ml @11:05:22.57 Pushing modified lysate into AIV Injection Loop @11:05:22.58 CBV.dial AIV @11:05:22.59 AIV.dial :bypass @11:05:22.60 PRV.dial CBV @11:05:22.62 PSV.dial :air @11:05:23.38 PS.seek 2.5ml @11:05:49.48 delay 3 seconds @11:05:52.50 PSV.dial :PML @11:05:52.97 PTV.dial :PRVmixing,avoiding: :puck @11:05:53.82 PS.reconfigure PSslow1 @11:05:53.87 PS.seek 1.74ml @11:07:11.17 delay 5 seconds @11:07:16.20 AIV.dial :sample @11:07:16.79 AWV.select :waste @11:07:16.96 PS.reconfigure PSslow2 @11:07:17.01 PS.seek 1.22ml @11:09:04.05 delay 15 seconds @11:09:19.08 AIV.dial :bypass @11:09:19.68 PS.reconfigure PSconfig @11:09:19.73 PS.seek 0ml @11:09:32.86 PSV.dial :air @11:09:33.32 PS.seek 2.5ml @11:09:59.42 PSV.dial :PML @11:09:59.89 PS.seek 0ml @11:10:26.02 AIV.dial :coilToMFB @11:10:26.03 AWV.select :air @11:10:26.20 Extracting Nucleic Acid from AIV @11:10:26.23
Beginning samplePassD @11:10:26.30 SPE.hold 2:46:40,55C @11:10:26.36 ASV.dial :SPEdelivery @11:10:26.44
AWV.select :air @11:10:26.45 AIV.dial :bypass @11:10:26.46 PSV.dial PSR @11:10:26.76 delay 20 seconds @11:10:27.62
Pulling 1.5ml of PSR.killMFB @11:10:27.63 PSR.select :killMFB @11:10:27.76 PS.seek 1.5ml @11:10:43.83 delay 5 seconds @11:10:46.79 primeLysateINT @11:10:46.80 AWV.select :air @11:10:46.81 AIV.dial :coilToMFB @11:10:46.83 ATV.dial AIV @11:10:48.55 AS.reconfigure ASslow1 @11:10:48.60 AS.seek 0.299ml @11:10:48.85
PSV.dial :waste1 @11:10:49.36 PS.seek 0ml @11:11:05.39 PSV.dial PSR @11:11:05.88 PSR.select :air @11:11:06.01 PS.seek 2.5ml @11:11:32.22 PRV.dial CBV @11:11:32.24
CBV.dial PRV @11:11:32.26 PSV.dial :mixloop @11:11:33.35 CTV.dial :air,avoiding: :intake @11:11:33.36 PTV.dial :PRVmixing @11:11:33.39 PS.seek 0ml @11:11:49.24 delay 10 seconds @11:11:59.27 ATV.dial :air2 @11:11:59.55
PSV.dial PSR @11:12:00.09 AS.seek 0.329ml @11:12:00.69
Pulling 1.5ml of PSR.flush @11:12:00.70 PSR.select :flush @11:12:00.83 PS.seek 1.5ml @11:12:06.40 ATV.dial :waste2 @11:12:06.96 AS.reconfigure ASnorm @11:12:07.01 AS.seek :empty @11:12:16.83
delay 5 seconds @11:12:21.86 PSV.dial :waste1 @11:12:22.48 PS.seek 0ml @11:12:24.01 Lysate primed! @11:12:24.02 AFV.select :holdingCoil @11:12:24.03 ATV.dial :air2 @11:12:24.66 AS.reconfigure ASslow1 @11:12:24.71 AS.seek 0.069ml @11:12:38.55
PSV.dial PSR @11:12:39.06 delay 3 seconds @11:12:39.14
PSR.select :air @11:12:39.27 PS.seek 2.5ml @11:12:42.09 ATV.dial :AIV @11:12:42.94 AS.seek 0.469ml @11:13:05.49 PRV.dial CBV @11:13:05.52
CBV.dial PRV @11:13:05.53 PSV.dial :mixloop @11:13:06.65 CTV.dial :air,avoiding: :intake @11:13:06.66 PTV.dial :PRVmixing @11:13:06.68 PS.seek 0ml @11:13:32.86 PSV.dial PSR @11:13:34.00 Pulling 1.5ml of PSR.flush @11:13:34.01 PSR.select :flush @11:13:34.14 PS.seek 1.5ml @11:13:50.15 delay 5 seconds @11:13:55.18 PSV.dial :waste1 @11:13:55.80 PS.seek 0ml @11:14:03.63 delay 5 seconds @11:14:08.72 Solid Phase Extraction Heater at 46.8C @11:14:08.73 ATV.dial ASV @11:14:09.14 ASV.dial :SPEdelivery @11:14:09.22 AS.seek 0.041ml @11:14:11.77
PSV.dial PSR @11:14:12.24 PSR.select :air @11:14:12.37 PS.seek 2.5ml @11:14:38.53 PRV.dial CBV @11:14:38.55
CBV.dial PRV @11:14:38.57 PSV.dial :mixloop @11:14:39.65 CTV.dial :air,avoiding: :intake @11:14:39.66 PTV.dial :PRVmixing @11:14:39.68 PS.seek 0ml @11:15:05.90 PSV.dial PSR @11:15:07.03 Pulling 1.5ml of PSR.flush @11:15:07.04 PSR.select :flush @11:15:07.17 PS.seek 1.5ml @11:15:23.15 delay 5 seconds @11:15:28.18 PSV.dial :waste1 @11:15:28.66 PS.seek 0ml @11:15:35.57 delay 10 seconds @11:15:44.71
PSV.dial PSR @11:15:45.18 PSR.select :air @11:15:45.30 PS.seek 2.5ml @11:15:45.59 rinseLysateLineINT @11:15:45.60 AFV.select :carrierSolution @11:15:45.71 delay 2 seconds @11:15:47.74 AS.reconfigure ASconfig @11:15:47.79 AS.seek 1ml @11:16:11.49 PRV.dial CBV @11:16:11.52
CBV.dial PRV @11:16:11.53 PSV.dial :mixloop @11:16:12.59 CTV.dial :air,avoiding: :intake @11:16:13.10 PTV.dial :PRVmixing @11:16:13.20 PS.reconfigure PSslow @11:16:13.25 PS.seek 1.2ml @11:16:40.05 PSV.dial :waste1 @11:16:41.41 PS.reconfigure PSconfig @11:16:41.45 PS.seek 0ml @11:16:43.22 delay 1 second @11:16:44.24 AFV.select :holdingCoil @11:16:44.34 delay 1 second @11:16:45.37 ATV.dial AIV @11:16:46.01 AIV.dial :coilToMFB @11:16:46.03 AWV.select :waste @11:16:46.14 AS.reconfigure ASslow0 @11:16:46.18 AS.seek 0ml @11:16:54.40
PSV.dial :air @11:16:55.47 PS.seek 2.5ml @11:17:21.68 PSV.dial :mixloop @11:17:22.14 PS.reconfigure PSslow @11:17:22.18 PS.seek 0ml @11:18:13.34 PSV.dial :air @11:18:13.83 PS.reconfigure PSconfig @11:18:13.87 PS.seek 2.5ml @11:18:27.08 ATV.dial :air2 @11:18:27.74 AS.reconfigure ASslow1 @11:18:27.79 AS.seek 0.05ml @11:18:38.12 ATV.dial :AIV @11:18:38.91 AS.seek 0.01ml @11:18:39.99
PSV.dial :mixloop @11:18:40.47 PS.reconfigure PSslow @11:18:40.51 PS.seek 0ml @11:18:47.25 ATV.dial :waste @11:18:48.81 AS.seek :empty @11:18:51.25 ethanol wash @11:18:51.26 ATV.dial :air2 @11:18:52.50 AS.reconfigure ASslow2 @11:18:52.55 AS.seek 0.009ml @11:19:02.84 delay 3 seconds @11:19:05.87 Pulling 0.4ml of Ethanol Wash @11:19:05.88 ATV.dial :ethanol @11:19:06.69 AS.reconfigure ASslow1 @11:19:06.74 AS.seek 0.409ml @11:19:31.59
Cleaning Collection Syringe @11:19:31.60 CSV.dial CSR @11:19:32.13 Pulling 4ml of CSR.flush @11:19:32.14 CSR.select :flush @11:19:32.27 CS.seek 4ml @11:19:43.49 delay 5 seconds @11:19:48.52 CSR.select :air @11:19:48.65 CS.seek 10ml @11:20:05.02 CSV.dial CTV @11:20:05.06
CTV.dial CSV @11:20:06.51 CBV.dial :waste1 @11:20:07.02 CS.seek 0ml @11:20:27.44 delay 3 seconds @11:20:30.47 ATV.dial :air1 @11:20:30.84 delay 3 seconds @11:20:33.52
CSV.dial CSR @11:20:33.92 AS.seek 0.434ml @11:20:34.05
Pulling 4ml of CSR.flush @11:20:34.06 CSR.select :flush @11:20:34.18 CS.seek 4ml @11:20:39.17 delay 3 seconds @11:20:42.20 ATV.dial :ethanol @11:20:42.79 AS.seek 0.419ml @11:20:45.37
delay 5 seconds @11:20:46.08 delay 3 seconds @11:20:49.10 ATV.dial ASV @11:20:50.39 AS.seek 0.092ml @11:20:50.44
CSR.select :air @11:20:50.56 CS.seek 10ml @11:21:06.96 CSV.dial CTV @11:21:06.99
CTV.dial CSV @11:21:07.48 CBV.dial :waste1 @11:21:07.50 CS.seek 0ml @11:21:34.06 Garaging FlushPuck in Collection Clamp @11:21:34.14 Elbow.reconfigure TightElbow @11:21:34.77 Elbow.seek CC @11:21:35.05 Elbow.reconfigure LooseElbow @11:21:35.15 Forearm.seek CC @11:21:36.16 Hand.close @11:21:36.85 CC.seek :open @11:21:41.22 Forearm.seek :retract @11:21:42.24 Elbow.reconfigure TightElbow @11:21:42.29 Elbow.seek :garage @11:21:45.47 Elbow.reconfigure LooseElbow @11:21:45.57 Forearm.reconfigure TightForearm @11:21:45.62 Forearm.seek :garage @11:21:46.79 Hand.open @11:21:47.53 Forearm.reconfigure LooseForearm @11:21:47.58 Forearm.seek :retract @11:21:48.60 Whole Cell Archive sampling 1500ml @11:21:48.62 Loading wcr puck from tube 2 into Collection Clamp @11:21:48.88 SC.reconfigure SCtight @11:21:48.93 SC.seek 2 @11:21:51.30 SC.reconfigure SCloose @11:21:51.53 Elbow.reconfigure TightElbow @11:21:51.58 Elbow.seek 2 @11:21:53.68 Forearm.reconfigure TightForearm @11:21:53.73 Forearm.seek 2 + 700 counts @11:21:54.78 SE.reconfigure SEpick @11:21:54.87 SE.seek beamBreak @11:21:56.41 delay 3 seconds @11:21:57.62 SE.configure SEconfig @11:21:57.79 Elbow.seek 2 @11:21:57.99 Forearm.seek 2 + 300 counts @11:21:58.24 Forearm.reconfigure LooseForearm @11:21:58.29 Hand.close @11:21:58.99 SE.seek :down @11:21:59.22 Forearm.seek :retract @11:21:59.43 ATV.dial :waste2 @11:22:00.18 AS.reconfigure ASconfig @11:22:00.22 AS.seek :empty @11:22:00.29 Elbow.seek CC @11:22:01.53 SE.coast @11:22:01.99 Elbow.reconfigure LooseElbow @11:22:02.10 Forearm.reconfigure TightForearm @11:22:02.15 Forearm.seek CC @11:22:03.51 CC.seek :closed @11:22:05.87 ATV.dial :air2 @11:22:06.96 AS.reconfigure ASslow1 @11:22:07.02 AS.seek 1ml @11:22:07.76 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.00 @11:22:07.78 Hand.open @11:22:08.53 Forearm.reconfigure LooseForearm @11:22:08.58 Forearm.seek :retract @11:22:09.67 SC.coast @11:22:09.71 collectSample 1500ml w/bubble point of 55psi @11:22:09.73 Priming 7ml @11:22:09.74 Intake.select :close @11:22:09.75 Exhaust.select :close @11:22:09.76 SS.hold @11:22:10.00 Calibrating pressure sensors @11:22:10.01 CTV.dial :air @11:22:11.40 SSV.dial :spit,avoiding: :exhaust @11:22:11.42 SS.reconfigure SSfast @11:22:11.47 SS.seek 5ml @11:22:14.13 SSV.dial CBV @11:22:14.17 CBV.dial SSV @11:22:15.65 delay 3.8 seconds @11:22:19.60 SSV.dial :spit @11:22:21.01 SS.seek 0ml @11:22:23.82 SSV.dial CBV @11:22:25.21 CTV.dial :intake @11:22:26.03 SSV.dial CBV @11:22:26.05 CBV.dial SSV @11:22:26.08 Allowing 40s for 7.0ml of remaining 7.0ml @11:22:26.09 Intake.select :open @11:22:28.27 Intake @32.0psia, vacuum from 20.0 to 24.0psi @11:22:28.29 SS.reconfigure SSinhale.with maxSpeed: 150 @11:22:28.34 SS.seek 7ml @11:22:34.48 Stroke completed -- waiting for pressure to equalize ... @11:22:47.83 Intake.select :close @11:22:47.99 SSV.dial :exhaust @11:22:48.81 Exhaust.select :open @11:22:48.93 SS.reconfigure SSexhale @11:22:48.98 SS.seek 0ml @11:22:52.79 Exhaust.select :close @11:22:52.90 Sampling 1500.0ml @11:22:52.92 Intake.select :close @11:22:52.93 Exhaust.select :close @11:22:52.94 SS.reconfigure SSconfig @11:22:52.98 SS.hold @11:22:53.78 CTV.dial :intake @11:22:53.84 SSV.dial CBV @11:22:53.86 CBV.dial SSV @11:22:54.68 Allowing 130s for 25.0ml of remaining 1500.0ml @11:22:54.69 Intake.select :open @11:22:56.87 Intake @32.0psia, vacuum from 20.0 to 24.0psi @11:22:56.89 SS.reconfigure SSinhale.with maxSpeed: 150 @11:22:56.94 SS.seek 25ml @11:23:00.20 Vacuum too high -- waiting up to 40s @11:23:02.59 SS.reconfigure SSinhale.with maxSpeed: 135 @11:23:02.65 SS.seek 25ml @11:23:05.54 Vacuum too high -- waiting up to 40s @11:23:07.94 SS.reconfigure SSinhale.with maxSpeed: 122 @11:23:07.99 SS.seek 25ml @11:23:10.64 Vacuum too high -- waiting up to 40s @11:23:13.00 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:23:13.02 SS.reconfigure SSinhale.with maxSpeed: 110 @11:23:13.07 SS.seek 25ml @11:23:15.83 Vacuum too high -- waiting up to 40s @11:23:18.18 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:23:18.20 SS.reconfigure SSinhale.with maxSpeed: 99 @11:23:18.25 SS.seek 25ml @11:23:21.15 Vacuum too high -- waiting up to 40s @11:23:23.10 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:23:23.12 SS.reconfigure SSinhale.with maxSpeed: 89 @11:23:23.16 SS.seek 25ml @11:23:25.61 Vacuum too high -- waiting up to 40s @11:23:27.76 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:23:27.78 SS.reconfigure SSinhale.with maxSpeed: 80 @11:23:27.82 SS.seek 25ml @11:23:30.58 Vacuum too high -- waiting up to 40s @11:23:32.73 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:23:32.75 SS.reconfigure SSinhale.with maxSpeed: 72 @11:23:32.80 SS.seek 25ml @11:23:35.67 Vacuum too high -- waiting up to 40s @11:23:37.51 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:23:37.53 SS.reconfigure SSinhale.with maxSpeed: 65 @11:23:37.58 SS.seek 25ml @11:23:41.31 Stroke completed -- waiting for pressure to equalize ... @11:23:46.42 Intake.select :close @11:23:46.59 SSV.dial :exhaust @11:23:47.41 Exhaust.select :open @11:23:47.53 SS.reconfigure SSexhale @11:23:47.58 SS.seek 0ml @11:23:56.62 Exhaust.select :close @11:23:56.74 SSV.dial CBV @11:23:56.76 CBV.dial SSV @11:23:57.60 Allowing 130s for 25.0ml of remaining 1475.0ml @11:23:57.61 Intake.select :open @11:23:59.80 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:23:59.81 SS.reconfigure SSinhale.with maxSpeed: 65 @11:23:59.86 SS.seek 25ml @11:24:05.23 Vacuum too high -- waiting up to 40s @11:24:07.44 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:24:07.46 SS.reconfigure SSinhale.with maxSpeed: 59 @11:24:07.51 SS.seek 25ml @11:24:12.89 Vacuum too high -- waiting up to 40s @11:24:14.56 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:24:14.58 SS.reconfigure SSinhale.with maxSpeed: 53 @11:24:14.63 SS.seek 25ml @11:24:21.45 Vacuum too high -- waiting up to 40s @11:24:23.31 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:24:23.33 SS.reconfigure SSinhale.with maxSpeed: 48 @11:24:23.38 SS.seek 25ml @11:24:31.67 Vacuum too high -- waiting up to 40s @11:24:33.28 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:24:33.30 SS.reconfigure SSinhale.with maxSpeed: 43 @11:24:33.35 SS.seek 25ml @11:24:44.69 Stroke completed -- waiting for pressure to equalize ... @11:24:49.82 Intake.select :close @11:24:49.98 SSV.dial :exhaust @11:24:50.81 Exhaust.select :open @11:24:50.93 SS.reconfigure SSexhale @11:24:50.98 SS.seek 0ml @11:25:00.06 Exhaust.select :close @11:25:00.18 SSV.dial CBV @11:25:00.20 CBV.dial SSV @11:25:01.04 Allowing 130s for 25.0ml of remaining 1450.0ml @11:25:01.05 Intake.select :open @11:25:03.23 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:25:03.25 SS.reconfigure SSinhale.with maxSpeed: 43 @11:25:03.30 SS.seek 25ml @11:25:21.86 Vacuum too high -- waiting up to 40s @11:25:23.47 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:25:23.49 SS.reconfigure SSinhale.with maxSpeed: 39 @11:25:23.54 SS.seek 25ml @11:25:28.64 delay 5 seconds @11:25:34.26 ATV.dial ASV @11:25:35.22 ASV.dial :SPEdelivery @11:25:35.24 AS.seek 0.02ml @11:25:48.76 Stroke completed -- waiting for pressure to equalize ... @11:25:53.92 Intake.select :close @11:25:54.08 SSV.dial :exhaust @11:25:54.88 Exhaust.select :open @11:25:55.00 SS.reconfigure SSexhale @11:25:55.05 SS.seek 0ml @11:26:03.87 Exhaust.select :close @11:26:03.99 SSV.dial CBV @11:26:04.01 CBV.dial SSV @11:26:04.85 Allowing 130s for 25.0ml of remaining 1425.0ml @11:26:04.86 Intake.select :open @11:26:07.05 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:26:07.07 SS.reconfigure SSinhale.with maxSpeed: 39 @11:26:07.12 SS.seek 25ml @11:26:52.71 Stroke completed -- waiting for pressure to equalize ... @11:26:58.63 Intake.select :close @11:26:58.79 SSV.dial :exhaust @11:26:59.60 Exhaust.select :open @11:26:59.72 SS.reconfigure SSexhale @11:26:59.77 SS.seek 0ml @11:27:08.57 Exhaust.select :close @11:27:08.69 SSV.dial CBV @11:27:08.71 CBV.dial SSV @11:27:09.53 Allowing 130s for 25.0ml of remaining 1400.0ml @11:27:09.54 Intake.select :open @11:27:11.73 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:27:11.75 SS.reconfigure SSinhale.with maxSpeed: 39 @11:27:11.79 SS.seek 25ml @11:27:51.11 Vacuum too high -- waiting up to 40s @11:27:52.98 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:27:53.00 SS.reconfigure SSinhale.with maxSpeed: 35 @11:27:53.05 SS.seek 25ml @11:28:00.19 Stroke completed -- waiting for pressure to equalize ... @11:28:04.67 Intake.select :close @11:28:05.42 SSV.dial :exhaust @11:28:06.26 Exhaust.select :open @11:28:06.39 SS.reconfigure SSexhale @11:28:06.45 SS.seek 0ml @11:28:15.48 Exhaust.select :close @11:28:15.60 SSV.dial CBV @11:28:15.62 CBV.dial SSV @11:28:16.46 Allowing 130s for 25.0ml of remaining 1375.0ml @11:28:16.47 Intake.select :open @11:28:18.66 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:28:18.68 SS.reconfigure SSinhale.with maxSpeed: 35 @11:28:18.73 SS.seek 25ml @11:28:52.61 delay 5 seconds @11:28:57.64 ATV.dial :air2 @11:28:58.64 AS.seek 0.52ml @11:29:09.04 Stroke completed -- waiting for pressure to equalize ... @11:29:14.18 Intake.select :close @11:29:14.34 SSV.dial :exhaust @11:29:15.17 Exhaust.select :open @11:29:15.29 SS.reconfigure SSexhale @11:29:15.34 SS.seek 0ml @11:29:24.31 Exhaust.select :close @11:29:24.43 SSV.dial CBV @11:29:24.45 CBV.dial SSV @11:29:25.29 Allowing 130s for 25.0ml of remaining 1350.0ml @11:29:25.30 Intake.select :open @11:29:27.49 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:29:27.51 SS.reconfigure SSinhale.with maxSpeed: 35 @11:29:27.56 SS.seek 25ml @11:30:18.23 Stroke completed -- waiting for pressure to equalize ... @11:30:23.34 Intake.select :close @11:30:23.50 SSV.dial :exhaust @11:30:24.32 Exhaust.select :open @11:30:24.44 SS.reconfigure SSexhale @11:30:24.49 SS.seek 0ml @11:30:33.24 Exhaust.select :close @11:30:33.36 SSV.dial CBV @11:30:33.38 CBV.dial SSV @11:30:34.20 Allowing 130s for 25.0ml of remaining 1325.0ml @11:30:34.21 Intake.select :open @11:30:36.40 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:30:36.42 SS.reconfigure SSinhale.with maxSpeed: 35 @11:30:36.47 SS.seek 25ml @11:30:39.49 delay 5 seconds @11:30:44.52 ATV.dial ASV @11:30:45.46 ASV.dial :wasteR @11:30:45.84 AS.seek 0.3ml @11:31:27.13 Stroke completed -- waiting for pressure to equalize ... @11:31:30.39 delay 20 seconds @11:31:32.94 Intake.select :close @11:31:33.10 SSV.dial :exhaust @11:31:33.92 Exhaust.select :open @11:31:34.04 SS.reconfigure SSexhale @11:31:34.09 SS.seek 0ml @11:31:43.25 Exhaust.select :close @11:31:43.36 SSV.dial CBV @11:31:43.40 CBV.dial SSV @11:31:44.23 Allowing 130s for 25.0ml of remaining 1300.0ml @11:31:44.24 Intake.select :open @11:31:46.43 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:31:46.45 SS.reconfigure SSinhale.with maxSpeed: 35 @11:31:46.49 SS.seek 25ml @11:31:50.43 AS.seek 0.07ml @11:32:35.99 Vacuum too high -- waiting up to 40s @11:32:36.94 delay 20 seconds @11:32:37.91 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:32:37.93 SS.reconfigure SSinhale.with maxSpeed: 32 @11:32:37.98 SS.seek 25ml @11:32:39.11 Stroke completed -- waiting for pressure to equalize ... @11:32:43.63 Intake.select :close @11:32:43.79 SSV.dial :exhaust @11:32:44.62 Exhaust.select :open @11:32:44.74 SS.reconfigure SSexhale @11:32:44.79 SS.seek 0ml @11:32:53.82 Exhaust.select :close @11:32:53.94 SSV.dial CBV @11:32:53.96 CBV.dial SSV @11:32:54.79 Allowing 130s for 25.0ml of remaining 1275.0ml @11:32:54.80 Intake.select :open @11:32:56.99 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:32:57.01 SS.reconfigure SSinhale.with maxSpeed: 32 @11:32:57.06 SS.seek 25ml @11:32:57.07 ATV.dial :waste2 @11:32:57.85 AS.reconfigure ASconfig @11:32:57.90 AS.seek :empty @11:33:02.27 Elution @11:33:02.28 ATV.dial :air1 @11:33:03.36 AS.reconfigure ASslow1 @11:33:03.41 AS.seek 0.049ml @11:33:13.76 delay 3 seconds @11:33:16.79 ATV.dial :ElutionWater @11:33:17.45 AS.seek 0.109ml @11:33:29.77 delay 3 seconds @11:33:32.80 ATV.dial ASV,via: :air2 @11:33:34.78 ASV.dial :SPEelution @11:33:35.35 AFV.select :carrierSolution @11:33:35.51 AS.reconfigure ASnorm @11:33:35.56 AS.seek 0.759ml @11:33:52.03 Stroke completed -- waiting for pressure to equalize ... @11:33:57.75 Intake.select :close @11:33:57.91 SSV.dial :exhaust @11:33:58.71 Exhaust.select :open @11:33:58.83 SS.reconfigure SSexhale @11:33:58.88 SS.seek 0ml @11:34:08.14 Exhaust.select :close @11:34:08.25 SSV.dial CBV @11:34:08.29 CBV.dial SSV @11:34:08.60 delay 3 seconds @11:34:09.12 Allowing 130s for 25.0ml of remaining 1250.0ml @11:34:09.13 Intake.select :open @11:34:11.31 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:34:11.33 SS.reconfigure SSinhale.with maxSpeed: 32 @11:34:11.38 SS.seek 25ml @11:34:11.62 AFV.select :holdingCoil @11:34:11.78 AS.reconfigure ASslow1 @11:34:11.83 AS.seek 0.474ml @11:35:06.39 Stroke completed -- waiting for pressure to equalize ... @11:35:09.41 delay 5 seconds @11:35:12.82 Intake.select :close @11:35:12.98 SSV.dial :exhaust @11:35:13.81 Exhaust.select :open @11:35:13.93 SS.reconfigure SSexhale @11:35:13.98 SS.seek 0ml @11:35:14.48 AS.reconfigure ASslow2 @11:35:14.54 AS.seek 0.439ml @11:35:23.06 Exhaust.select :close @11:35:23.18 SSV.dial CBV @11:35:23.20 CBV.dial SSV @11:35:24.04 Allowing 130s for 25.0ml of remaining 1225.0ml @11:35:24.05 Intake.select :open @11:35:26.23 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:35:26.25 SS.reconfigure SSinhale.with maxSpeed: 32 @11:35:26.30 SS.seek 25ml @11:35:49.87 delay 1:00 @11:36:21.28 Stroke completed -- waiting for pressure to equalize ... @11:36:28.30 Intake.select :close @11:36:28.46 SSV.dial :exhaust @11:36:29.26 Exhaust.select :open @11:36:29.38 SS.reconfigure SSexhale @11:36:29.43 SS.seek 0ml @11:36:38.28 Exhaust.select :close @11:36:38.40 SSV.dial CBV @11:36:38.42 CBV.dial SSV @11:36:39.26 Allowing 130s for 25.0ml of remaining 1200.0ml @11:36:39.27 Intake.select :open @11:36:41.45 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:36:41.47 SS.reconfigure SSinhale.with maxSpeed: 32 @11:36:41.52 SS.seek 25ml @11:36:49.90 pushing eluate to holding coil @11:36:49.97 AS.seek 0.279ml @11:37:22.36 Vacuum too high -- waiting up to 40s @11:37:24.59 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:37:24.61 SS.reconfigure SSinhale.with maxSpeed: 29 @11:37:24.65 SS.seek 25ml @11:37:40.79 Stroke completed -- waiting for pressure to equalize ... @11:37:47.22 Intake.select :close @11:37:47.38 SSV.dial :exhaust @11:37:48.20 Exhaust.select :open @11:37:48.32 SS.reconfigure SSexhale @11:37:48.37 SS.seek 0ml @11:37:57.23 Exhaust.select :close @11:37:57.35 SSV.dial CBV @11:37:57.37 CBV.dial SSV @11:37:58.20 Allowing 130s for 25.0ml of remaining 1175.0ml @11:37:58.21 Intake.select :open @11:38:00.40 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:38:00.42 SS.reconfigure SSinhale.with maxSpeed: 29 @11:38:00.47 SS.seek 25ml @11:39:01.34 Stroke completed -- waiting for pressure to equalize ... @11:39:08.99 Intake.select :close @11:39:09.15 SSV.dial :exhaust @11:39:09.95 Exhaust.select :open @11:39:10.07 SS.reconfigure SSexhale @11:39:10.12 SS.seek 0ml @11:39:19.20 Exhaust.select :close @11:39:19.32 SSV.dial CBV @11:39:19.34 CBV.dial SSV @11:39:20.19 Allowing 130s for 25.0ml of remaining 1150.0ml @11:39:20.20 Intake.select :open @11:39:22.39 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:39:22.41 SS.reconfigure SSinhale.with maxSpeed: 29 @11:39:22.46 SS.seek 25ml @11:39:31.03 delay 30 seconds @11:40:01.06 ATV.dial :waste,via: :air3 @11:40:02.67 AS.reconfigure ASconfig @11:40:02.72 AS.seek :empty @11:40:19.11 Parking Bleach in LysateSource line and SPEcolumn @11:40:19.12 ATV.dial :air3 @11:40:19.36 Vacuum too high -- waiting up to 40s @11:40:19.97 AS.reconfigure ASslow1 @11:40:20.02 AS.seek 0.029ml @11:40:21.89 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:40:21.91 SS.reconfigure SSinhale.with maxSpeed: 26 @11:40:21.96 SS.seek 25ml @11:40:26.33 Stroke completed -- waiting for pressure to equalize ... @11:40:27.08 ATV.dial :ElutionWater @11:40:27.70 AS.seek 0.059ml @11:40:32.11 Intake.select :close @11:40:32.27 SSV.dial :exhaust @11:40:33.09 Exhaust.select :open @11:40:33.21 SS.reconfigure SSexhale @11:40:33.26 SS.seek 0ml @11:40:34.01 ATV.dial :air3 @11:40:34.61 AS.seek 0.069ml @11:40:36.87 delay 2 seconds @11:40:38.89 ATV.dial :bleach @11:40:39.33 AS.seek 0.769ml @11:40:42.53 Exhaust.select :close @11:40:42.65 SSV.dial CBV @11:40:42.68 CBV.dial SSV @11:40:43.50 Allowing 130s for 25.0ml of remaining 1125.0ml @11:40:43.51 Intake.select :open @11:40:45.70 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:40:45.72 SS.reconfigure SSinhale.with maxSpeed: 26 @11:40:45.77 SS.seek 25ml @11:41:52.87 Stroke completed -- waiting for pressure to equalize ... @11:41:59.88 Intake.select :close @11:42:00.04 SSV.dial :exhaust @11:42:00.84 Exhaust.select :open @11:42:00.96 SS.reconfigure SSexhale @11:42:01.01 SS.seek 0ml @11:42:10.39 Exhaust.select :close @11:42:10.51 SSV.dial CBV @11:42:10.53 CBV.dial SSV @11:42:11.36 Allowing 130s for 25.0ml of remaining 1100.0ml @11:42:11.37 Intake.select :open @11:42:13.56 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:42:13.58 SS.reconfigure SSinhale.with maxSpeed: 26 @11:42:13.63 SS.seek 25ml @11:43:00.37 delay 3 seconds @11:43:03.40 ATV.dial :air3 @11:43:03.94 delay 3 seconds @11:43:07.03 AS.seek 0.794ml @11:43:12.26 delay 3 seconds @11:43:15.88 ATV.dial :bleach @11:43:16.51 AS.seek 0.779ml @11:43:19.83 delay 3 seconds @11:43:21.15 Stroke completed -- waiting for pressure to equalize ... @11:43:22.85 ATV.dial :AIV @11:43:24.05 AS.reconfigure ASconfig @11:43:24.10 AS.seek 0.015ml @11:43:30.11 Intake.select :close @11:43:30.27 SSV.dial :exhaust @11:43:31.08 Exhaust.select :open @11:43:31.20 SS.reconfigure SSexhale @11:43:31.25 SS.seek 0ml @11:43:40.45 Exhaust.select :close @11:43:40.57 SSV.dial CBV @11:43:40.59 CBV.dial SSV @11:43:41.42 Allowing 130s for 25.0ml of remaining 1075.0ml @11:43:41.43 Intake.select :open @11:43:43.62 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:43:43.64 SS.reconfigure SSinhale.with maxSpeed: 26 @11:43:43.69 SS.seek 25ml @11:44:08.33 ATV.dial :waste @11:44:09.73 AS.seek :empty @11:44:10.94 ATV.dial :air3 @11:44:11.60 AS.reconfigure ASslow1 @11:44:11.65 AS.seek 0.009ml @11:44:13.97 delay 2 seconds @11:44:15.99 ATV.dial :bleach @11:44:16.46 AS.seek 0.579ml @11:44:26.85 Vacuum too high -- waiting up to 40s @11:44:29.46 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:44:29.48 SS.reconfigure SSinhale.with maxSpeed: 23 @11:44:29.53 SS.seek 25ml @11:44:57.19 Stroke completed -- waiting for pressure to equalize ... @11:45:05.48 Intake.select :close @11:45:05.64 SSV.dial :exhaust @11:45:06.47 Exhaust.select :open @11:45:06.59 SS.reconfigure SSexhale @11:45:06.64 SS.seek 0ml @11:45:15.75 Exhaust.select :close @11:45:15.87 SSV.dial CBV @11:45:15.89 CBV.dial SSV @11:45:16.73 Allowing 130s for 25.0ml of remaining 1050.0ml @11:45:16.74 Intake.select :open @11:45:18.93 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:45:18.95 SS.reconfigure SSinhale.with maxSpeed: 23 @11:45:19.01 SS.seek 25ml @11:46:11.36 delay 3 seconds @11:46:14.39 ATV.dial :air3 @11:46:14.92 delay 3 seconds @11:46:18.01 AS.seek 0.604ml @11:46:23.32 delay 3 seconds @11:46:26.35 ATV.dial :bleach @11:46:26.95 AS.seek 0.589ml @11:46:30.36 delay 3 seconds @11:46:33.39 ATV.dial :ASV @11:46:34.38 ASV.dial :wasteR @11:46:34.96 AS.seek 0ml @11:46:35.03 Stroke completed -- waiting for pressure to equalize ... @11:46:44.59 Intake.select :close @11:46:44.75 SSV.dial :exhaust @11:46:45.56 Exhaust.select :open @11:46:45.68 SS.reconfigure SSexhale @11:46:45.74 SS.seek 0ml @11:46:54.92 Exhaust.select :close @11:46:55.04 SSV.dial CBV @11:46:55.06 CBV.dial SSV @11:46:55.89 Allowing 130s for 25.0ml of remaining 1025.0ml @11:46:55.90 Intake.select :open @11:46:58.07 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:46:58.09 SS.reconfigure SSinhale.with maxSpeed: 23 @11:46:58.14 SS.seek 25ml @11:47:51.27 Vacuum too high -- waiting up to 40s @11:47:54.71 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:47:54.75 SS.reconfigure SSinhale.with maxSpeed: 21 @11:47:54.80 SS.seek 25ml @11:48:20.84 Stroke completed -- waiting for pressure to equalize ... @11:48:29.83 Intake.select :close @11:48:29.99 SSV.dial :exhaust @11:48:30.81 Exhaust.select :open @11:48:30.93 SS.reconfigure SSexhale @11:48:30.99 SS.seek 0ml @11:48:33.75 delay 1:00 @11:48:40.20 Exhaust.select :close @11:48:40.32 SSV.dial CBV @11:48:40.34 CBV.dial SSV @11:48:41.17 Allowing 130s for 25.0ml of remaining 1000.0ml @11:48:41.18 Intake.select :open @11:48:43.37 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:48:43.39 SS.reconfigure SSinhale.with maxSpeed: 21 @11:48:43.43 SS.seek 25ml @11:49:33.78 AFV.select :carrierSolution @11:49:33.88 delay 2 seconds @11:49:35.91 AS.reconfigure ASconfig @11:49:35.96 AS.seek 1ml @11:49:56.12 Vacuum too high -- waiting up to 40s @11:49:59.49 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:49:59.51 SS.reconfigure SSinhale.with maxSpeed: 19 @11:49:59.56 SS.seek 25ml @11:50:11.83 Stroke completed -- waiting for pressure to equalize ... @11:50:21.33 Intake.select :close @11:50:21.49 SSV.dial :exhaust @11:50:22.31 Exhaust.select :open @11:50:22.43 SS.reconfigure SSexhale @11:50:22.48 SS.seek 0ml @11:50:31.69 Exhaust.select :close @11:50:31.80 SSV.dial CBV @11:50:31.84 CBV.dial SSV @11:50:32.67 Allowing 130s for 25.0ml of remaining 975.0ml @11:50:32.69 Intake.select :open @11:50:33.72 delay 1 second @11:50:34.74 AFV.select :holdingCoil @11:50:34.87 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:50:34.89 SS.reconfigure SSinhale.with maxSpeed: 19 @11:50:34.94 SS.seek 25ml @11:50:34.97 delay 1 second @11:50:36.00 ATV.dial ASV @11:50:36.01 ASV.dial :wasteR @11:50:36.03 AS.reconfigure ASslow1 @11:50:36.08 AS.seek 0ml @11:52:07.25 Stroke completed -- waiting for pressure to equalize ... @11:52:20.58 Intake.select :close @11:52:20.74 SSV.dial :exhaust @11:52:21.56 Exhaust.select :open @11:52:21.68 SS.reconfigure SSexhale @11:52:21.73 SS.seek 0ml @11:52:30.72 Exhaust.select :close @11:52:30.83 SSV.dial CBV @11:52:30.86 CBV.dial SSV @11:52:31.69 Allowing 130s for 25.0ml of remaining 950.0ml @11:52:31.70 Intake.select :open @11:52:33.88 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:52:33.90 SS.reconfigure SSinhale.with maxSpeed: 19 @11:52:33.95 SS.seek 25ml @11:53:32.96 Vacuum too high -- waiting up to 40s @11:53:36.95 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:53:36.98 SS.reconfigure SSinhale.with maxSpeed: 17 @11:53:37.03 SS.seek 25ml @11:53:57.47 delay 20 seconds @11:54:15.05 Stroke completed -- waiting for pressure to equalize ... @11:54:17.59 ATV.dial :air3 @11:54:19.03 AS.seek 0.59ml @11:54:27.26 Intake.select :close @11:54:27.42 SSV.dial :exhaust @11:54:28.24 Exhaust.select :open @11:54:28.36 SS.reconfigure SSexhale @11:54:28.41 SS.seek 0ml @11:54:37.69 Exhaust.select :close @11:54:37.81 SSV.dial CBV @11:54:37.83 CBV.dial SSV @11:54:38.69 Allowing 130s for 25.0ml of remaining 925.0ml @11:54:38.70 Intake.select :open @11:54:40.88 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:54:40.90 SS.reconfigure SSinhale.with maxSpeed: 17 @11:54:40.95 SS.seek 25ml @11:56:10.31 Vacuum too high -- waiting up to 40s @11:56:14.46 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:56:14.48 SS.reconfigure SSinhale.with maxSpeed: 15 @11:56:14.53 SS.seek 25ml @11:56:18.01 delay 3 seconds @11:56:21.04 ATV.dial :ASV @11:56:22.37 ASV.dial :wasteR @11:56:22.45 AS.seek 0.09ml @11:56:30.67 Stroke completed -- waiting for pressure to equalize ... @11:56:43.41 Intake.select :close @11:56:43.57 SSV.dial :exhaust @11:56:44.39 Exhaust.select :open @11:56:44.51 SS.reconfigure SSexhale @11:56:44.56 SS.seek 0ml @11:56:53.82 Exhaust.select :close @11:56:53.94 SSV.dial CBV @11:56:53.96 CBV.dial SSV @11:56:54.80 Allowing 130s for 25.0ml of remaining 900.0ml @11:56:54.81 Intake.select :open @11:56:57.00 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:56:57.02 SS.reconfigure SSinhale.with maxSpeed: 15 @11:56:57.07 SS.seek 25ml @11:58:03.27 delay 30 seconds @11:58:33.30 ATV.dial :extSrc @11:58:34.04 delay 3 seconds @11:58:37.07 ATV.dial :ElutionWater @11:58:38.14 AS.seek 0.12ml @11:58:44.46 ATV.dial :air3 @11:58:45.06 AS.seek 0.13ml @11:58:47.32 delay 2 seconds @11:58:49.34 ATV.dial :bleach @11:58:49.82 AS.seek 0.23ml @11:58:53.95 Stroke completed -- waiting for pressure to equalize ... @11:59:04.82 SS.reconfigure SSconfig @11:59:04.87 SS.hold @11:59:05.00 Intake.select :close @11:59:05.16 ShallowSampler::Clogged in WCR -- progress too slow after 625.0ml @11:59:05.18 SSV.dial :exhaust @11:59:06.61 Exhaust.select :open @11:59:06.73 SS.reconfigure SSexhale @11:59:06.78 SS.seek 0ml @11:59:10.22 delay 3 seconds @11:59:13.24 ATV.dial :air3 @11:59:13.77 delay 3 seconds @11:59:16.24 Exhaust.select :close @11:59:16.35 Sampled 625.0ml @11:59:16.37 Drying puck before fixing for 4:00 @11:59:16.38 CBV.dial :waste2b @11:59:16.85 AS.seek 0.255ml @11:59:17.20 delay 1:00 @11:59:22.20 delay 3 seconds @11:59:25.23 ATV.dial :bleach @11:59:25.84 AS.seek 0.24ml @11:59:29.20 delay 3 seconds @11:59:32.23 ATV.dial :ASV @11:59:33.23 ASV.dial :SPEdelivery @11:59:33.60 AFV.select :carrierSolution @11:59:33.71 delay 2 seconds @11:59:35.74 AS.reconfigure ASconfig @11:59:35.79 AS.seek 0.5ml @11:59:51.02 delay 1 second @11:59:52.04 AFV.select :holdingCoil @11:59:52.19 AS.reconfigure ASslow1 @11:59:52.25 AS.seek 0.17ml @12:00:17.23 CSV.dial :air @12:00:17.72 CS.reconfigure CSfast @12:00:17.77 CS.seek 10ml @12:00:41.63 Pumping wcr puck from tube 2 up to 55psi... @12:00:41.65 CBV.dial SSV @12:00:43.03 CBV.dial between CSV and waste2b @12:00:44.06 CSV.dial between air and CTV @12:00:44.53 CS.seek 7.092ml @12:00:52.21 CTV.dial CSV,avoiding: :air @12:00:52.23 CSV.dial CTV @12:00:53.08 CS.reconfigure CSpressurize @12:00:53.13 CS.seek 0ml @12:00:58.91 SPE.hold 2:46:40,95C @12:00:58.95 delay 3:00 @12:01:10.41 CBV.dial :waste2b @12:01:10.78 CS.reconfigure CSconfig @12:01:10.83 CS.seek :empty @12:01:13.10 delay 1:00 @12:02:13.13 CTV.dial :air,avoiding: :intake @12:02:14.58 Fix #1 of 2 (with rnal) for 2:00 @12:02:14.59 CSV.dial CSR @12:02:15.13 Pulling 2ml of CSR.rnal @12:02:15.15 CSR.select :rnal @12:02:15.27 CS.seek 2ml @12:02:21.39 delay 5 seconds @12:02:26.42 CSR.select :air @12:02:26.54 CS.reconfigure CSfast @12:02:26.59 CS.seek 10ml @12:02:46.57 Pumping wcr puck from tube 2 up to 55psi... @12:02:46.59 CBV.dial SSV @12:02:47.97 CBV.dial between CSV and waste2b @12:02:49.03 CSV.dial between air and CTV @12:02:49.69 CS.seek 7.169ml @12:02:57.49 CTV.dial CSV,avoiding: :air @12:02:57.51 CSV.dial CTV @12:02:59.02 CS.reconfigure CSpressurize @12:02:59.07 CS.seek 0ml @12:03:17.47 delay 2:00 @12:03:59.54 AFV.select :carrierSolution @12:03:59.68 delay 2 seconds @12:04:01.71 AS.reconfigure ASconfig @12:04:01.76 AS.seek 1ml @12:04:49.76 delay 1 second @12:04:50.78 AFV.select :holdingCoil @12:04:50.88 delay 1 second @12:04:51.91 ASV.dial :wasteD @12:04:52.90 AS.seek 0ml @12:05:17.50 CBV.dial :waste2b @12:05:17.86 CS.reconfigure CSconfig @12:05:17.92 CS.seek :empty @12:05:19.30 delay 1:00 @12:05:50.67 Priming Eluate @12:05:50.68 AFV.select :carrierSolution @12:05:50.79 delay 2 seconds @12:05:52.83 AS.seek 0.5ml @12:06:19.33 CTV.dial :air,avoiding: :intake @12:06:20.76 Fix #2 of 2 (with rnal) for 2:00 @12:06:20.77 CSV.dial CSR @12:06:21.32 Pulling 2ml of CSR.rnal @12:06:21.33 CSR.select :rnal @12:06:21.45 CS.seek 2ml @12:06:21.90 delay 1 second @12:06:22.92 AFV.select :holdingCoil @12:06:23.02 ATV.dial ASV @12:06:23.03 ASV.dial :SPErecovery @12:06:23.61 Swish #1 of 2 @12:06:23.69 AS.reconfigure ASslow0 @12:06:23.74 AS.seek 0.6ml @12:06:27.58 delay 5 seconds @12:06:32.61 CSR.select :air @12:06:32.74 CS.reconfigure CSfast @12:06:32.79 CS.seek 10ml @12:06:34.21 AS.seek 0.5ml @12:06:44.60 Swish #2 of 2 @12:06:44.69 AS.seek 0.6ml @12:06:52.30 Pumping wcr puck from tube 2 up to 55psi... @12:06:52.32 CBV.dial SSV @12:06:53.70 CBV.dial between CSV and waste2b @12:06:54.74 CSV.dial between air and CTV @12:06:55.16 AS.seek 0.5ml @12:06:55.40 CS.seek 7.143ml @12:07:03.06 CTV.dial CSV,avoiding: :air @12:07:03.08 CSV.dial CTV @12:07:04.56 CS.reconfigure CSpressurize @12:07:04.61 CS.seek 0ml @12:07:05.56 delay 3 seconds @12:07:08.64 AS.reconfigure ASslow1 @12:07:08.69 AS.seek 0.684ml @12:07:22.26 delay 2:00 @12:07:45.98 delay 3 seconds @12:07:49.01 Eluate primed @12:07:49.02 ATV.dial :waste2 @12:07:49.61 AS.reconfigure ASnorm @12:07:49.66 AS.seek 0ml @12:08:24.46 SPE.coast @12:08:24.55 ASV.dial :SPErecovery @12:08:24.57 Setting up PCR for OM -- writing /var/log/esp/OM20may1812h400ml.pcr @12:08:24.59 Building PCR Slug from ASV with MM3 mix, PP5 primer @12:08:24.60 Priming with carrier @12:08:24.61 ATV.dial ARV @12:08:25.56 ARV.dial :carrier @12:08:25.91 AS.seek 0.5ml @12:08:51.47 AFV.select :carrierSolution @12:08:51.58 delay 2 seconds @12:08:53.61 AS.reconfigure ASconfig @12:08:53.66 AS.seek 1ml @12:09:22.29 CBV.dial :waste2b @12:09:22.65 CS.reconfigure CSconfig @12:09:22.74 delay 1 second @12:09:22.76 CS.seek :empty @12:09:23.76 AFV.select :holdingCoil @12:09:23.87 delay 1 second @12:09:24.90 ATV.dial :waste @12:09:25.15 delay 1:00 @12:09:26.33 AS.reconfigure ASnorm @12:09:26.39 AS.seek :empty @12:10:17.14 AFV.select :carrierSolution @12:10:17.25 delay 2 seconds @12:10:19.28 AS.reconfigure ASconfig @12:10:19.94 AS.seek 0.5ml @12:10:25.18 CTV.dial :air,avoiding: :intake @12:10:26.64 Storing wcr puck in Collection Clamp to tube 1 @12:10:26.84 SC.reconfigure SCtight @12:10:26.89 SC.seek 1 @12:10:28.98 SC.reconfigure SCloose @12:10:29.22 Elbow.reconfigure TightElbow @12:10:29.28 Elbow.seek 1 @12:10:31.27 Forearm.reconfigure TightForearm @12:10:31.32 Forearm.seek 1 + 700 counts @12:10:32.46 SE.reconfigure SEpick @12:10:32.54 SE.seek beamBreak @12:10:34.14 SE.configure SEconfig @12:10:34.32 Elbow.reconfigure LimpElbow @12:10:34.37 Forearm.reconfigure LooseForearm @12:10:34.42 Forearm.seek 1 @12:10:34.74 Elbow.hold @12:10:34.82 Elbow.reconfigure TightElbow @12:10:34.93 SE.seek 16.1 Pucks below Top Plate @12:10:35.50 Forearm.force -20 @12:10:35.55 Forearm.seek 1 + 334 counts @12:10:35.89 Forearm.reconfigure TightForearm @12:10:35.94 Forearm.seek 1 + 700 counts @12:10:36.24 SE.seek 17.1 Pucks below Top Plate @12:10:36.97 Forearm.reconfigure LooseForearm @12:10:37.02 Forearm.seek :retract @12:10:37.92 Elbow.seek CC @12:10:39.65 Elbow.reconfigure LooseElbow @12:10:39.75 Forearm.seek CC @12:10:40.80 Hand.close @12:10:41.43 CC.seek :open @12:10:45.94 Forearm.seek :retract @12:10:47.01 Elbow.reconfigure TightElbow @12:10:47.06 Elbow.seek 1 @12:10:48.97 Elbow.reconfigure LimpElbow @12:10:49.05 delay 1 second @12:10:49.08 Forearm.seek 1 @12:10:50.11 Elbow.hold @12:10:50.17 AFV.select :holdingCoil @12:10:50.24 Elbow.reconfigure TightElbow @12:10:50.29 Hand.open @12:10:50.34 Building Slug @12:10:50.35 ATV.dial :ARV @12:10:51.67 Forearm.force -20 @12:10:51.72 Forearm.seek 1 + 346 counts @12:10:51.94 delay 2 seconds @12:10:52.12 Forearm.reconfigure TightForearm @12:10:52.18 Forearm.seek 1 + 700 counts @12:10:52.54 SE.seek :down @12:10:53.70 SE.coast @12:10:53.72 Forearm.reconfigure LooseForearm @12:10:53.82 Forearm.seek :retract @12:10:53.97 ARV.dial :air1 @12:10:54.36 delay 2 seconds @12:10:54.74 SC.coast @12:10:54.79 Starting finalPurge @12:10:54.80 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:10:54.88 Hand.open @12:10:55.32 Elbow.seek FlushPuck::Garage @12:10:56.45 AS.reconfigure ASslow2 @12:10:56.51 AS.seek 0.506ml @12:10:57.35
Elbow.reconfigure LooseElbow @12:10:57.46 Forearm.seek :garage @12:10:58.42 Hand.close @12:10:59.19 Forearm.seek :retract @12:11:00.22 Elbow.reconfigure TightElbow @12:11:00.27 Elbow.seek CC @12:11:02.76 delay 2 seconds @12:11:03.15
Elbow.reconfigure LooseElbow @12:11:03.25 Forearm.reconfigure TightForearm @12:11:03.30 Forearm.seek CC @12:11:04.64 CC.seek :closed @12:11:04.81 ARV.dial :MM3 @12:11:05.81 delay 2 seconds @12:11:07.85 Adding MM3 @12:11:07.98 AS.seek 0.524ml @12:11:08.90
Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.94 @12:11:08.98 Hand.open @12:11:09.62 Forearm.reconfigure LooseForearm @12:11:09.67 Forearm.seek :retract @12:11:10.70 Flushing the Collection Valve::Manifold @12:11:10.84 CSV.dial CSR @12:11:11.36 Pulling 5ml of CSR.flush @12:11:11.37 CSR.select :flush @12:11:11.50 CS.seek 5ml @12:11:25.30 delay 5 seconds @12:11:26.34 delay 25 seconds @12:11:30.33
CSR.select :air @12:11:30.51 CS.reconfigure CSfast @12:11:30.55 CS.seek 7.5ml @12:11:37.24 CSV.dial :waste @12:11:39.21 CS.reconfigure CSconfig @12:11:39.27 CS.seek 0ml @12:11:51.37 ARV.dial :PP5 @12:11:51.75 Adding PP5 @12:11:51.77 delay 2 seconds @12:11:53.85 AS.seek 0.53ml @12:11:59.34
Flushing lower manifold's waste1 port @12:11:59.48 CSV.dial :flush @12:11:59.95 CS.reconfigure CSfast @12:11:59.99 CS.seek 5ml @12:12:00.23 delay 10 seconds @12:12:10.26 ARV.dial :air3 @12:12:10.64 adding ARV air @12:12:10.66 delay 2 seconds @12:12:12.49
delay 5 seconds @12:12:12.75 AS.seek 0.536ml @12:12:17.51
CSV.dial :air @12:12:18.06 CS.seek 7ml @12:12:19.11 delay 5 seconds @12:12:23.65 CSV.dial CTV @12:12:23.68
CTV.dial CSV @12:12:24.13 ARV.dial :carrier @12:12:25.13
CBV.dial :waste1 @12:12:25.36 delay 7 seconds @12:12:25.96
CS.seek 0ml @12:12:32.45 AS.seek 0.594ml @12:12:42.68
Flushing lower manifold's waste2b port @12:12:42.82 CSV.dial :flush @12:12:43.60 CS.seek 5ml @12:12:55.87 delay 5 seconds @12:13:00.90 CSV.dial :air @12:13:01.45 CS.seek 7ml @12:13:07.06 CSV.dial CTV @12:13:07.09
CTV.dial CSV @12:13:07.56 CBV.dial :waste2b @12:13:08.38 CS.seek 0ml @12:13:25.02 Flushing upper manifold @12:13:25.16 CSV.dial :flush @12:13:25.94 CS.seek 5ml @12:13:31.16 delay 5 seconds @12:13:36.19 ATV.dial :ASV @12:13:36.58 delay 2 seconds @12:13:38.21
delay 5 seconds @12:13:38.66 AS.seek 0.6ml @12:13:43.23
CSV.dial :air @12:13:43.78 CS.seek 7ml @12:13:44.92 delay 4 seconds @12:13:48.95 ATV.dial ARV @12:13:49.34 CSV.dial CBV @12:13:49.38
CBV.dial CSV @12:13:49.50 delay 2 seconds @12:13:50.49
CTV.dial :air @12:13:51.53 ARV.dial :carrier @12:13:51.54 delay 2 seconds @12:13:51.88
CS.seek 0ml @12:13:53.63 AS.seek 0.65ml @12:14:08.59
delay 7 seconds @12:14:15.63 CS.seek 3ml @12:14:23.37 CSV.dial :waste @12:14:23.86 CS.seek 0ml @12:14:31.77 CSV.dial :flush @12:14:32.24 CS.seek 3ml @12:14:40.09 delay 5 seconds @12:14:44.05 delay 4 seconds @12:14:45.11
CSV.dial :air @12:14:45.64 CS.seek 5ml @12:14:48.08 ATV.dial PCR @12:14:48.67 ASV.dial :waste1 @12:14:50.06 ARV.dial :air1 @12:14:50.46 Positioning Slug by 0.324ml @12:14:50.55 AS.seek 0.326ml @12:14:51.21
CSV.dial :air @12:14:51.23 CS.seek 0ml @12:15:03.46 delay 7 seconds @12:15:10.50 CS.seek 3ml @12:15:18.25 CSV.dial :waste @12:15:19.02 CS.seek 0ml @12:15:26.81 Disinfecting external Intake with mfbkill @12:15:26.95 CSV.dial CSR @12:15:28.36 Pulling 8ml of CSR.mfbkill @12:15:28.37 CSR.select :mfbkill @12:15:28.49 CS.seek 8ml @12:15:47.47 delay 5 seconds @12:15:52.50 CSR.select :air @12:15:52.63 CS.seek 10ml @12:15:58.33 CSV.dial CBV @12:15:58.35
CBV.dial CSV @12:15:59.40 CTV.dial :intake @12:16:00.20 Intake.select :open @12:16:00.32 CS.seek 2ml @12:16:20.47 Intake.select :close @12:16:20.58 CTV.dial :air @12:16:21.37 CS.reconfigure CSconfig @12:16:21.42 CS.seek 0ml @12:16:27.47 Flushing lower manifold's waste1 port @12:16:27.48 CSV.dial CSR @12:16:28.61 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @12:16:28.74 CS.seek 4ml @12:16:39.93 delay 5 seconds @12:16:44.96 CSR.select :air @12:16:45.09 CS.reconfigure CSfast @12:16:45.13 CS.seek 10ml @12:16:59.59 CSV.dial CTV @12:16:59.62
CTV.dial CSV @12:17:01.09 CBV.dial :waste1 @12:17:01.59 CS.seek 0ml @12:17:24.87 Flushing lower manifold's waste2b port @12:17:24.88 CSV.dial CSR @12:17:25.43 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @12:17:25.56 CS.reconfigure CSconfig @12:17:25.61 CS.seek 4ml @12:17:36.81 delay 5 seconds @12:17:41.84 CSR.select :air @12:17:41.97 CS.reconfigure CSfast @12:17:42.01 CS.seek 10ml @12:17:56.50 CSV.dial CTV @12:17:56.53
CTV.dial CSV @12:17:57.03 CBV.dial :waste2b @12:17:57.85 CS.seek 0ml @12:18:21.16 Killing upper manifold @12:18:21.17 CSV.dial CSR @12:18:21.72 Pulling 4ml of CSR.mfbkill @12:18:21.73 CSR.select :mfbkill @12:18:21.86 CS.reconfigure CSconfig @12:18:21.91 CS.seek 4ml @12:18:33.19 delay 5 seconds @12:18:38.22 CSR.select :air @12:18:38.35 CS.reconfigure CSfast @12:18:38.39 CS.seek 10ml @12:18:52.97 CSV.dial CBV @12:18:53.00
CBV.dial CSV @12:18:54.11 CTV.dial :air @12:18:55.49 CS.seek 0ml @12:19:18.81 delay 7 seconds @12:19:25.85 CS.seek 3ml @12:19:33.59 CSV.dial :waste @12:19:34.18 CS.seek 0ml @12:19:41.97 Flushing upper manifold @12:19:42.11 CSV.dial :flush @12:19:42.59 CS.seek 5ml @12:19:54.75 delay 5 seconds @12:20:00.38 CSV.dial :air @12:20:00.95 CS.seek 7ml @12:20:06.53 CSV.dial CBV @12:20:06.56
CBV.dial CSV @12:20:07.63 CTV.dial :air @12:20:07.65 CS.seek 0ml @12:20:16.74 delay 5 seconds @12:20:21.77 ATV.dial ARV @12:20:22.50 ASV.dial :PCRlock @12:20:22.86 delay 5 seconds @12:20:24.28
delay 7 seconds @12:20:27.89 Running OM Profile60C350 thermocycling @12:20:27.91 PCR.program Profile60C350 @12:20:28.02 Profile60C350 should complete in < 2:02:45 @12:20:28.03 PCR.run 2:38:26.25 @12:20:31.31
CS.seek 3ml @12:20:39.10 CSV.dial :waste @12:20:39.59 CS.seek 0ml @12:20:47.54 CSV.dial :flush @12:20:48.01 CS.seek 3ml @12:20:55.91 delay 5 seconds @12:21:00.93 CSV.dial :air @12:21:01.48 CS.seek 5ml @12:21:07.10 CSV.dial :air @12:21:07.12 CS.seek 0ml @12:21:19.42 delay 7 seconds @12:21:26.45 CS.seek 3ml @12:21:34.32 CSV.dial :waste @12:21:35.11 CS.seek 0ml @12:21:42.94 Flushing lower manifold's waste1 port @12:21:43.08 CSV.dial :flush @12:21:43.58 CS.seek 5ml @12:21:55.88 delay 5 seconds @12:22:00.90 CSV.dial :air @12:22:01.43 CS.seek 7ml @12:22:06.97 CSV.dial CTV @12:22:07.01
CTV.dial CSV @12:22:08.46 CBV.dial :waste1 @12:22:08.95 CS.seek 0ml @12:22:25.94 Flushing lower manifold's waste2b port @12:22:26.08 CSV.dial :flush @12:22:26.87 CS.seek 5ml @12:22:39.19 delay 5 seconds @12:22:44.21 CSV.dial :air @12:22:44.76 CS.seek 7ml @12:22:50.32 CSV.dial CTV @12:22:50.35
CTV.dial CSV @12:22:50.83 CBV.dial :waste2b @12:22:51.64 CS.seek 0ml @12:23:08.41 Disinfecting external Intake with kill @12:23:08.55 CSV.dial CSR @12:23:09.07 Pulling 8ml of CSR.kill @12:23:09.08 CSR.select :kill @12:23:09.21 CS.seek 8ml @12:23:28.22 delay 5 seconds @12:23:33.24 CSR.select :air @12:23:33.37 CS.seek 10ml @12:23:38.91 CSV.dial CBV @12:23:38.94
CBV.dial CSV @12:23:40.71 CTV.dial :intake @12:23:41.51 Intake.select :open @12:23:41.63 CS.seek 2ml @12:24:02.01 Intake.select :close @12:24:02.12 CTV.dial :air @12:24:02.91 CS.reconfigure CSconfig @12:24:02.97 CS.seek 0ml @12:24:09.23 CSV.dial CSR @12:24:10.36 Pulling 5ml of CSR.flush @12:24:10.37 CSR.select :flush @12:24:10.50 CS.seek 5ml @12:24:24.23 delay 5 seconds @12:24:29.26 CSR.select :air @12:24:29.39 CS.seek 10ml @12:24:43.23 CSV.dial CBV @12:24:44.30 CS.seek 0ml @12:25:10.82 Garaging FlushPuck in Collection Clamp @12:25:10.90 Elbow.reconfigure TightElbow @12:25:10.96 Elbow.seek CC @12:25:11.22 Elbow.reconfigure LooseElbow @12:25:11.32 Forearm.seek CC @12:25:12.34 Hand.close @12:25:12.96 CC.seek :open @12:25:17.33 Forearm.seek :retract @12:25:18.31 Elbow.reconfigure TightElbow @12:25:18.36 Elbow.seek :garage @12:25:21.49 Elbow.reconfigure LooseElbow @12:25:21.60 Forearm.reconfigure TightForearm @12:25:21.65 Forearm.seek :garage @12:25:22.81 Hand.open @12:25:23.52 Forearm.reconfigure LooseForearm @12:25:23.57 Forearm.seek :retract @12:25:24.59 Starting initialPurge @12:25:24.60 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:25:24.68 Hand.open @12:25:25.02 Elbow.reconfigure TightElbow @12:25:25.07 Elbow.seek FlushPuck::Garage @12:25:25.19 Elbow.reconfigure LooseElbow @12:25:25.30 Forearm.seek :garage @12:25:26.28 Hand.close @12:25:27.08 Forearm.seek :retract @12:25:28.08 Elbow.reconfigure TightElbow @12:25:28.13 Elbow.seek CC @12:25:30.96 Elbow.reconfigure LooseElbow @12:25:31.07 Forearm.reconfigure TightForearm @12:25:31.12 Forearm.seek CC @12:25:32.44 CC.seek :closed @12:25:36.69 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.91 @12:25:37.43 Hand.open @12:25:38.15 Forearm.reconfigure LooseForearm @12:25:38.20 Forearm.seek :retract @12:25:39.23 Flushing the Collection Valve::Manifold @12:25:39.37 CSV.dial CSR @12:25:40.50 Pulling 5ml of CSR.flush CSR.select :flush @12:25:40.63 CS.seek 5ml @12:25:54.48 delay 5 seconds @12:25:59.50 CSR.select :air @12:25:59.67 CS.reconfigure CSfast @12:25:59.73 CS.seek 7.5ml @12:26:06.32 CSV.dial :waste @12:26:07.65 CS.reconfigure CSconfig @12:26:07.71 CS.seek 0ml @12:26:27.85 Purging external Intake @12:26:27.99 CSV.dial :flush @12:26:28.46 CS.reconfigure CSfast @12:26:28.52 CS.seek 10ml @12:26:51.98 delay 5 seconds @12:26:57.00 CSV.dial CBV @12:26:57.04
CBV.dial CSV @12:26:57.82 CTV.dial :intake @12:26:58.60 Intake.select :open @12:26:58.72 CS.seek 0ml @12:27:23.64 Intake.select :close @12:27:23.75 CTV.dial :air @12:27:24.54 Flushing upper manifold @12:27:24.68 CSV.dial :flush @12:27:25.46 CS.seek 5ml @12:27:37.70 delay 5 seconds @12:27:42.72 CSV.dial :air @12:27:43.27 CS.seek 7ml @12:27:48.76 CSV.dial CBV @12:27:48.78
CBV.dial CSV @12:27:49.87 CTV.dial :air @12:27:49.89 CS.seek 0ml @12:28:06.64 delay 7 seconds @12:28:13.67 CS.seek 3ml @12:28:21.45 CSV.dial :waste @12:28:21.93 CS.seek 0ml @12:28:29.79 CSV.dial :flush @12:28:30.27 CS.seek 3ml @12:28:38.11 delay 5 seconds @12:28:43.13 CSV.dial :air @12:28:43.66 CS.seek 5ml @12:28:49.23 CSV.dial :air @12:28:49.25 CS.seek 0ml @12:29:01.64 delay 7 seconds @12:29:08.67 CS.seek 3ml @12:29:16.36 CSV.dial :waste @12:29:17.15 CS.seek 0ml @12:29:25.01 Garaging FlushPuck in Collection Clamp @12:29:25.09 Elbow.reconfigure TightElbow @12:29:25.14 Elbow.seek CC @12:29:25.39 Elbow.reconfigure LooseElbow @12:29:25.50 Forearm.seek CC @12:29:26.50 Hand.close @12:29:27.08 CC.seek :open @12:29:32.13 Forearm.seek :retract @12:29:33.14 Elbow.reconfigure TightElbow @12:29:33.19 Elbow.seek :garage @12:29:36.40 Elbow.reconfigure LooseElbow @12:29:36.50 Forearm.reconfigure TightForearm @12:29:36.55 Forearm.seek :garage @12:29:37.72 Hand.open @12:29:38.40 Forearm.reconfigure LooseForearm @12:29:38.45 Forearm.seek :retract @12:29:39.49 Completed [stdphase] begun 10:01:37 @12:29:39.50 Preparing to sleep until Tue May 19 10:01:37 +0200 2020 @12:29:39.56 deferred until MFBPCR finish @12:29:39.58
Waiting for MFBPCR til 10:01CEST19-May-20 @12:29:39.60 Continuing at 10:01CEST19-May-20 @12:29:39.66 GoodNews.email "Then, in tube 2: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:124\n{1 => 18, 2 => 4}\nCan@12:29:39, 9.2C, 44% humidity, 14.4psia, 13.594V, 0.840A, 0.840A avg, 11.42W",Subject: "Waiting until 10:01CEST19-May-20" @13:57:03.66 PCR.off @13:57:04.93 PCR for OM completed -- closed /var/log/esp/OM20may1812h400ml.pcr @13:57:05.00 Cleaning PCR @13:57:05.01 AFV.select :carrierSolution @13:57:05.12 delay 2 seconds @13:57:07.15 AS.reconfigure ASconfig @13:57:07.20 AS.seek 0.5ml @13:57:17.49 delay 1 second @13:57:18.51 AFV.select :holdingCoil @13:57:18.61 ASV.dial :waste1 @13:57:18.99 ATV.dial :air3 @13:57:20.22 AS.reconfigure ASslow1 @13:57:20.27 AS.seek 0.51ml @13:57:22.58 delay 2 seconds @13:57:24.60 ATV.dial :bleach @13:57:25.20 AS.seek 0.81ml @13:58:25.77 delay 3 seconds @13:58:28.80 ATV.dial :air3 @13:58:29.33 delay 3 seconds @13:58:32.42 AS.seek 0.835ml @13:58:37.71 delay 3 seconds @13:58:40.74 ATV.dial :bleach @13:58:41.34 AS.seek 0.82ml @13:58:44.63 delay 3 seconds @13:58:47.65 ARV.dial :waste @13:58:48.24 delay 3 seconds @13:58:51.27 ATV.dial :ARV @13:58:52.12 AS.reconfigure ASconfig @13:58:52.16 AS.seek 0.47ml @13:59:12.54 AFV.select :carrierSolution @13:59:12.65 delay 2 seconds @13:59:15.33 AS.seek 1ml @13:59:46.12 delay 1 second @13:59:47.14 AFV.select :holdingCoil @13:59:47.24 delay 1 second @13:59:48.27 AS.reconfigure ASfast @13:59:48.32 AS.seek 0ml @14:00:15.66 AFV.select :carrierSolution @14:00:15.77 delay 2 seconds @14:00:17.80 AS.reconfigure ASconfig @14:00:17.85 AS.seek 1ml @14:01:15.63 delay 1 second @14:01:16.65 AFV.select :holdingCoil @14:01:16.75 delay 1 second @14:01:17.78 AS.reconfigure ASfast @14:01:17.83 AS.seek 0ml @14:01:45.12 AFV.select :carrierSolution @14:01:45.23 delay 2 seconds @14:01:47.26 AS.reconfigure ASconfig @14:01:47.31 AS.seek 1ml @14:02:45.09 delay 1 second @14:02:46.11 AFV.select :holdingCoil @14:02:46.21 delay 1 second @14:02:47.24 AS.reconfigure ASfast @14:02:47.29 AS.seek 0ml @14:03:14.63 ARV.dial between carrier and air1 @14:03:15.10 ATV.dial :air3 @14:03:16.35 AS.reconfigure ASslow1 @14:03:16.40 AS.seek 0.01ml @14:03:18.70 delay 2 seconds @14:03:20.72 ATV.dial :bleach @14:03:21.33 AS.seek 0.81ml @14:06:02.45 delay 3 seconds @14:06:05.48 ATV.dial :air3 @14:06:06.01 delay 3 seconds @14:06:09.10 AS.seek 0.835ml @14:06:14.41 delay 3 seconds @14:06:17.44 ATV.dial :bleach @14:06:18.05 AS.seek 0.82ml @14:06:21.36 delay 3 seconds @14:06:24.39 ATV.dial PCR @14:06:24.79 AS.reconfigure ASconfig @14:06:24.84 AS.seek :empty @14:07:12.31 PCR.program :bleachProfile @14:07:12.37 PCR program should complete in < 2:45 @14:07:12.38 PCR.run 10:00 @14:10:26.33 PCR.off @14:10:26.35 AFV.select :carrierSolution @14:10:26.46 delay 2 seconds @14:10:28.50 AS.seek 1ml @14:11:26.36 delay 1 second @14:11:27.38 AFV.select :holdingCoil @14:11:27.48 delay 1 second @14:11:28.51 AS.reconfigure ASfast @14:11:28.56 AS.seek 0ml @14:11:55.89 delay 3 seconds @14:11:58.92 AFV.select :carrierSolution @14:11:59.02 delay 2 seconds @14:12:01.05 AS.reconfigure ASconfig @14:12:01.10 AS.seek 1ml @14:12:58.89 delay 1 second @14:12:59.91 AFV.select :holdingCoil @14:13:00.01 delay 1 second @14:13:01.04 AS.reconfigure ASfast @14:13:01.09 AS.seek 0ml @14:13:28.44 delay 3 seconds @14:13:31.47 AFV.select :carrierSolution @14:13:31.57 delay 2 seconds @14:13:33.60 AS.reconfigure ASconfig @14:13:33.66 AS.seek 1ml @14:14:31.49 delay 1 second @14:14:32.51 AFV.select :holdingCoil @14:14:32.61 delay 1 second @14:14:33.64 AS.reconfigure ASfast @14:14:33.69 AS.seek 0ml @14:15:01.05 delay 3 seconds @14:15:04.08 AFV.select :carrierSolution @14:15:04.18 delay 2 seconds @14:15:06.21 AS.reconfigure ASconfig @14:15:06.27 AS.seek 1ml @14:16:04.05 delay 1 second @14:16:05.07 AFV.select :holdingCoil @14:16:05.17 delay 1 second @14:16:06.20 AS.reconfigure ASfast @14:16:06.25 AS.seek 0ml @14:16:33.60 delay 3 seconds @14:16:36.63 AFV.select :carrierSolution @14:16:36.73 delay 2 seconds @14:16:38.76 AS.reconfigure ASconfig @14:16:38.82 AS.seek 1ml @14:17:36.60 delay 1 second @14:17:37.62 AFV.select :holdingCoil @14:17:37.72 delay 1 second @14:17:38.75 AS.reconfigure ASnorm @14:17:38.80 AS.seek 0ml @14:18:29.51 Setting up PCR for ML -- writing /var/log/esp/ML20may1814h400ml.pcr @14:18:29.53 Building PCR Slug from ASV with MM2 mix, PP4 primer @14:18:29.55 Priming with carrier @14:18:29.56 ATV.dial ARV @14:18:30.15 ARV.dial :carrier @14:18:31.15 AS.seek 0.5ml @14:18:56.71 AFV.select :carrierSolution @14:18:56.82 delay 2 seconds @14:18:58.85 AS.reconfigure ASconfig @14:18:58.90 AS.seek 1ml @14:19:27.97 delay 1 second @14:19:28.99 AFV.select :holdingCoil @14:19:29.09 delay 1 second @14:19:30.12 ATV.dial :waste @14:19:31.69 AS.reconfigure ASnorm @14:19:31.75 AS.seek :empty @14:20:22.49 AFV.select :carrierSolution @14:20:22.60 delay 2 seconds @14:20:24.63 AS.reconfigure ASconfig @14:20:24.68 AS.seek 0.5ml @14:20:53.76 delay 1 second @14:20:54.78 AFV.select :holdingCoil @14:20:54.88 Building Slug @14:20:54.89 ATV.dial :ARV @14:20:56.44 delay 2 seconds @14:20:58.47 ARV.dial :air1 @14:20:58.86 delay 2 seconds @14:21:00.95 AS.reconfigure ASslow2 @14:21:01.01 AS.seek 0.506ml @14:21:07.24 delay 2 seconds @14:21:09.27 ARV.dial :MM2 @14:21:10.66 delay 2 seconds @14:21:12.69 Adding MM2 @14:21:12.76 AS.seek 0.524ml @14:21:31.10 delay 25 seconds @14:21:56.13 ARV.dial :PP4 @14:21:56.51 Adding PP4 @14:21:56.53 delay 2 seconds @14:21:58.62 AS.seek 0.53ml @14:22:05.23 delay 10 seconds @14:22:15.26 ARV.dial :air3 @14:22:15.65 adding ARV air @14:22:15.67 delay 2 seconds @14:22:17.75 AS.seek 0.536ml @14:22:24.19 delay 5 seconds @14:22:29.22 ARV.dial :carrier @14:22:30.41 delay 7 seconds @14:22:37.50 AS.seek 0.594ml @14:23:36.26 delay 5 seconds @14:23:41.29 ATV.dial :ASV @14:23:41.68 delay 2 seconds @14:23:43.77 AS.seek 0.6ml @14:23:50.13 delay 4 seconds @14:23:54.16 ATV.dial ARV @14:23:54.70 delay 2 seconds @14:23:56.73 ARV.dial :carrier @14:23:56.74 delay 2 seconds @14:23:58.83 AS.seek 0.65ml @14:24:49.29 delay 4 seconds @14:24:53.32 ATV.dial PCR @14:24:53.90 ASV.dial :waste1 @14:24:53.91 ARV.dial :air1 @14:24:54.32 Positioning Slug by 0.324ml @14:24:54.41 AS.seek 0.326ml @14:30:20.61 delay 5 seconds @14:30:25.64 ATV.dial ARV @14:30:26.48 ASV.dial :PCRlock @14:30:26.87 delay 5 seconds @14:30:31.90 Running ML Profile60C350 thermocycling @14:30:31.91 PCR.program Profile60C350 @14:30:32.02 Profile60C350 should complete in < 2:02:45 @14:30:32.04 PCR.run 2:38:26.25 @16:06:58.03 PCR.off @16:06:59.30 PCR for ML completed -- closed /var/log/esp/ML20may1814h400ml.pcr @16:06:59.37 Cleaning PCR @16:06:59.38 AFV.select :carrierSolution @16:06:59.49 delay 2 seconds @16:07:01.52 AS.reconfigure ASconfig @16:07:01.57 AS.seek 0.5ml @16:07:11.86 delay 1 second @16:07:12.88 AFV.select :holdingCoil @16:07:12.98 ASV.dial :waste1 @16:07:13.36 ATV.dial :air3 @16:07:14.57 AS.reconfigure ASslow1 @16:07:14.62 AS.seek 0.51ml @16:07:16.89 delay 2 seconds @16:07:18.91 ATV.dial :bleach @16:07:19.51 AS.seek 0.81ml @16:08:20.08 delay 3 seconds @16:08:23.11 ATV.dial :air3 @16:08:23.65 delay 3 seconds @16:08:26.74 AS.seek 0.835ml @16:08:32.03 delay 3 seconds @16:08:35.06 ATV.dial :bleach @16:08:35.67 AS.seek 0.82ml @16:08:38.99 delay 3 seconds @16:08:42.02 ARV.dial :waste @16:08:42.60 delay 3 seconds @16:08:45.63 ATV.dial :ARV @16:08:46.47 AS.reconfigure ASconfig @16:08:46.53 AS.seek 0.47ml @16:09:06.93 AFV.select :carrierSolution @16:09:07.05 delay 2 seconds @16:09:09.09 AS.seek 1ml @16:09:39.87 delay 1 second @16:09:40.89 AFV.select :holdingCoil @16:09:40.99 delay 1 second @16:09:42.02 AS.reconfigure ASfast @16:09:42.07 AS.seek 0ml @16:10:09.41 AFV.select :carrierSolution @16:10:09.52 delay 2 seconds @16:10:11.55 AS.reconfigure ASconfig @16:10:11.60 AS.seek 1ml @16:11:09.40 delay 1 second @16:11:10.42 AFV.select :holdingCoil @16:11:10.52 delay 1 second @16:11:11.55 AS.reconfigure ASfast @16:11:11.60 AS.seek 0ml @16:11:38.94 AFV.select :carrierSolution @16:11:39.05 delay 2 seconds @16:11:41.08 AS.reconfigure ASconfig @16:11:41.13 AS.seek 1ml @16:12:38.91 delay 1 second @16:12:39.93 AFV.select :holdingCoil @16:12:40.03 delay 1 second @16:12:41.06 AS.reconfigure ASfast @16:12:41.11 AS.seek 0ml @16:13:08.46 ARV.dial between carrier and air1 @16:13:08.95 ATV.dial :air3 @16:13:10.19 AS.reconfigure ASslow1 @16:13:10.24 AS.seek 0.01ml @16:13:12.57 delay 2 seconds @16:13:14.59 ATV.dial :bleach @16:13:15.20 AS.seek 0.81ml @16:15:57.03 delay 3 seconds @16:16:00.06 ATV.dial :air3 @16:16:00.60 delay 3 seconds @16:16:03.69 AS.seek 0.835ml @16:16:09.02 delay 3 seconds @16:16:12.05 ATV.dial :bleach @16:16:12.66 AS.seek 0.82ml @16:16:15.98 delay 3 seconds @16:16:19.01 ATV.dial PCR @16:16:19.40 AS.reconfigure ASconfig @16:16:19.46 AS.seek :empty @16:17:06.92 PCR.program :bleachProfile @16:17:06.98 PCR program should complete in < 2:45 @16:17:06.99 PCR.run 10:00 @16:20:20.93 PCR.off @16:20:20.96 AFV.select :carrierSolution @16:20:21.07 delay 2 seconds @16:20:23.11 AS.seek 1ml @16:21:20.97 delay 1 second @16:21:21.99 AFV.select :holdingCoil @16:21:22.09 delay 1 second @16:21:23.12 AS.reconfigure ASfast @16:21:23.17 AS.seek 0ml @16:21:50.50 delay 3 seconds @16:21:53.53 AFV.select :carrierSolution @16:21:53.63 delay 2 seconds @16:21:55.66 AS.reconfigure ASconfig @16:21:56.25 AS.seek 1ml @16:22:54.03 delay 1 second @16:22:55.05 AFV.select :holdingCoil @16:22:55.15 delay 1 second @16:22:56.18 AS.reconfigure ASfast @16:22:56.23 AS.seek 0ml @16:23:23.58 delay 3 seconds @16:23:26.61 AFV.select :carrierSolution @16:23:26.71 delay 2 seconds @16:23:28.74 AS.reconfigure ASconfig @16:23:28.80 AS.seek 1ml @16:24:26.61 delay 1 second @16:24:27.63 AFV.select :holdingCoil @16:24:27.73 delay 1 second @16:24:28.76 AS.reconfigure ASfast @16:24:28.81 AS.seek 0ml @16:24:56.16 delay 3 seconds @16:24:59.19 AFV.select :carrierSolution @16:24:59.29 delay 2 seconds @16:25:01.32 AS.reconfigure ASconfig @16:25:01.38 AS.seek 1ml @16:25:59.19 delay 1 second @16:26:00.21 AFV.select :holdingCoil @16:26:00.31 delay 1 second @16:26:01.34 AS.reconfigure ASfast @16:26:01.39 AS.seek 0ml @16:26:28.72 delay 3 seconds @16:26:31.75 AFV.select :carrierSolution @16:26:31.85 delay 2 seconds @16:26:33.88 AS.reconfigure ASconfig @16:26:33.94 AS.seek 1ml @16:27:31.75 delay 1 second @16:27:32.77 AFV.select :holdingCoil @16:27:32.87 delay 1 second @16:27:33.90 AS.reconfigure ASnorm @16:27:33.95 AS.seek 0ml @16:28:24.68 Setting up PCR for CG -- writing /var/log/esp/CG20may1816h400ml.pcr @16:28:24.70 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:28:24.71 Priming with carrier @16:28:24.72 ATV.dial ARV @16:28:25.30 ARV.dial :carrier @16:28:25.65 AS.seek 0.5ml @16:28:51.18 AFV.select :carrierSolution @16:28:51.29 delay 2 seconds @16:28:53.32 AS.reconfigure ASconfig @16:28:53.37 AS.seek 1ml @16:29:22.40 delay 1 second @16:29:23.42 AFV.select :holdingCoil @16:29:23.52 delay 1 second @16:29:24.55 ATV.dial :waste @16:29:26.13 AS.reconfigure ASnorm @16:29:26.18 AS.seek :empty @16:30:16.96 AFV.select :carrierSolution @16:30:17.07 delay 2 seconds @16:30:19.10 AS.reconfigure ASconfig @16:30:19.15 AS.seek 0.5ml @16:30:48.22 delay 1 second @16:30:49.24 AFV.select :holdingCoil @16:30:49.34 Building Slug @16:30:49.35 ATV.dial :ARV @16:30:50.91 delay 2 seconds @16:30:52.94 ARV.dial :air1 @16:30:53.33 delay 2 seconds @16:30:55.42 AS.reconfigure ASslow2 @16:30:55.47 AS.seek 0.506ml @16:31:01.85 delay 2 seconds @16:31:03.87 ARV.dial :MM1 @16:31:04.44 delay 2 seconds @16:31:06.47 Adding MM1 @16:31:06.54 AS.seek 0.524ml @16:31:25.49 delay 25 seconds @16:31:50.52 ARV.dial :PP2 @16:31:50.90 Adding PP2 @16:31:50.92 delay 2 seconds @16:31:53.01 AS.seek 0.53ml @16:31:59.27 delay 10 seconds @16:32:09.30 ARV.dial :air2 @16:32:09.66 adding ARV air @16:32:09.68 delay 2 seconds @16:32:11.76 AS.seek 0.536ml @16:32:18.12 delay 5 seconds @16:32:23.15 ARV.dial :carrier @16:32:24.35 delay 7 seconds @16:32:31.44 AS.seek 0.594ml @16:33:29.96 delay 5 seconds @16:33:34.99 ATV.dial :ASV @16:33:35.38 delay 2 seconds @16:33:37.47 AS.seek 0.6ml @16:33:43.71 delay 4 seconds @16:33:47.74 ATV.dial ARV @16:33:48.27 delay 2 seconds @16:33:50.30 ARV.dial :carrier @16:33:50.31 delay 2 seconds @16:33:52.40 AS.seek 0.65ml @16:34:42.87 delay 4 seconds @16:34:46.90 ATV.dial PCR @16:34:47.49 ASV.dial :waste1 @16:34:47.50 ARV.dial :air1 @16:34:47.88 Positioning Slug by 0.324ml @16:34:47.97 AS.seek 0.326ml @16:40:13.98 delay 5 seconds @16:40:19.01 ATV.dial ARV @16:40:19.75 ASV.dial :PCRlock @16:40:20.14 delay 5 seconds @16:40:25.17 Running CG Profile60C300 thermocycling @16:40:25.18 PCR.program Profile60C300 @16:40:25.29 Profile60C300 should complete in < 1:59:25 @16:40:25.30 PCR.run 2:34:16.25 @18:15:24.41 PCR.off @18:15:25.07 PCR for CG completed -- closed /var/log/esp/CG20may1816h400ml.pcr @18:15:25.14 Cleaning PCR @18:15:25.15 AFV.select :carrierSolution @18:15:25.26 delay 2 seconds @18:15:27.29 AS.reconfigure ASconfig @18:15:27.34 AS.seek 0.5ml @18:15:37.63 delay 1 second @18:15:38.65 AFV.select :holdingCoil @18:15:39.38 ASV.dial :waste1 @18:15:39.80 ATV.dial :air3 @18:15:41.03 AS.reconfigure ASslow1 @18:15:41.08 AS.seek 0.51ml @18:15:43.36 delay 2 seconds @18:15:45.38 ATV.dial :bleach @18:15:45.99 AS.seek 0.81ml @18:16:46.57 delay 3 seconds @18:16:49.60 ATV.dial :air3 @18:16:50.14 delay 3 seconds @18:16:53.23 AS.seek 0.835ml @18:16:58.50 delay 3 seconds @18:17:01.53 ATV.dial :bleach @18:17:02.14 AS.seek 0.82ml @18:17:05.44 delay 3 seconds @18:17:08.47 ARV.dial :waste @18:17:09.05 delay 3 seconds @18:17:12.08 ATV.dial :ARV @18:17:13.04 AS.reconfigure ASconfig @18:17:13.09 AS.seek 0.47ml @18:17:33.50 AFV.select :carrierSolution @18:17:33.61 delay 2 seconds @18:17:35.65 AS.seek 1ml @18:18:06.39 delay 1 second @18:18:07.41 AFV.select :holdingCoil @18:18:07.51 delay 1 second @18:18:08.54 AS.reconfigure ASfast @18:18:08.59 AS.seek 0ml @18:18:35.91 AFV.select :carrierSolution @18:18:36.02 delay 2 seconds @18:18:38.05 AS.reconfigure ASconfig @18:18:38.10 AS.seek 1ml @18:19:35.86 delay 1 second @18:19:36.88 AFV.select :holdingCoil @18:19:36.98 delay 1 second @18:19:38.01 AS.reconfigure ASfast @18:19:38.06 AS.seek 0ml @18:20:05.38 AFV.select :carrierSolution @18:20:05.49 delay 2 seconds @18:20:07.52 AS.reconfigure ASconfig @18:20:07.57 AS.seek 1ml @18:21:05.33 delay 1 second @18:21:06.35 AFV.select :holdingCoil @18:21:06.45 delay 1 second @18:21:07.48 AS.reconfigure ASfast @18:21:07.53 AS.seek 0ml @18:21:34.87 ARV.dial between carrier and air1 @18:21:35.35 ATV.dial :air3 @18:21:36.58 AS.reconfigure ASslow1 @18:21:36.63 AS.seek 0.01ml @18:21:38.91 delay 2 seconds @18:21:40.93 ATV.dial :bleach @18:21:41.53 AS.seek 0.81ml @18:24:22.66 delay 3 seconds @18:24:25.69 ATV.dial :air3 @18:24:26.23 delay 3 seconds @18:24:29.32 AS.seek 0.835ml @18:24:34.63 delay 3 seconds @18:24:37.65 ATV.dial :bleach @18:24:38.25 AS.seek 0.82ml @18:24:41.54 delay 3 seconds @18:24:44.57 ATV.dial PCR @18:24:44.96 AS.reconfigure ASconfig @18:24:45.01 AS.seek :empty @18:25:32.46 PCR.program :bleachProfile @18:25:32.52 PCR program should complete in < 2:45 @18:25:32.53 PCR.run 10:00 @18:28:46.47 PCR.off @18:28:46.49 AFV.select :carrierSolution @18:28:46.60 delay 2 seconds @18:28:48.64 AS.seek 1ml @18:29:46.45 delay 1 second @18:29:47.47 AFV.select :holdingCoil @18:29:47.57 delay 1 second @18:29:48.60 AS.reconfigure ASfast @18:29:48.65 AS.seek 0ml @18:30:15.98 delay 3 seconds @18:30:19.01 AFV.select :carrierSolution @18:30:19.11 delay 2 seconds @18:30:21.14 AS.reconfigure ASconfig @18:30:21.19 AS.seek 1ml @18:31:18.98 delay 1 second @18:31:20.00 AFV.select :holdingCoil @18:31:20.10 delay 1 second @18:31:21.13 AS.reconfigure ASfast @18:31:21.18 AS.seek 0ml @18:31:48.51 delay 3 seconds @18:31:52.10 AFV.select :carrierSolution @18:31:52.29 delay 2 seconds @18:31:54.32 AS.reconfigure ASconfig @18:31:54.38 AS.seek 1ml @18:32:52.14 delay 1 second @18:32:53.16 AFV.select :holdingCoil @18:32:53.26 delay 1 second @18:32:54.29 AS.reconfigure ASfast @18:32:54.34 AS.seek 0ml @18:33:21.70 delay 3 seconds @18:33:24.73 AFV.select :carrierSolution @18:33:24.83 delay 2 seconds @18:33:26.86 AS.reconfigure ASconfig @18:33:26.92 AS.seek 1ml @18:34:24.72 delay 1 second @18:34:25.74 AFV.select :holdingCoil @18:34:25.84 delay 1 second @18:34:26.87 AS.reconfigure ASfast @18:34:26.92 AS.seek 0ml @18:34:54.27 delay 3 seconds @18:34:57.30 AFV.select :carrierSolution @18:34:57.40 delay 2 seconds @18:34:59.43 AS.reconfigure ASconfig @18:34:59.48 AS.seek 1ml @18:35:57.30 delay 1 second @18:35:58.32 AFV.select :holdingCoil @18:35:58.42 delay 1 second @18:35:59.45 AS.reconfigure ASnorm @18:35:59.50 AS.seek 0ml @18:36:50.21 Setting up PCR for NM -- writing /var/log/esp/NM20may1818h400ml.pcr @18:36:50.23 Building PCR Slug from ASV with MM1 mix, PP1 primer @18:36:50.24 Priming with carrier @18:36:50.25 ATV.dial ARV @18:36:50.85 ARV.dial :carrier @18:36:51.20 AS.seek 0.5ml @18:37:16.77 AFV.select :carrierSolution @18:37:16.88 delay 2 seconds @18:37:18.91 AS.reconfigure ASconfig @18:37:18.96 AS.seek 1ml @18:37:48.00 delay 1 second @18:37:49.02 AFV.select :holdingCoil @18:37:49.12 delay 1 second @18:37:50.15 ATV.dial :waste @18:37:51.72 AS.reconfigure ASnorm @18:37:51.78 AS.seek :empty @18:38:42.52 AFV.select :carrierSolution @18:38:42.63 delay 2 seconds @18:38:44.66 AS.reconfigure ASconfig @18:38:44.71 AS.seek 0.5ml @18:39:13.78 delay 1 second @18:39:14.80 AFV.select :holdingCoil @18:39:14.90 Building Slug @18:39:14.91 ATV.dial :ARV @18:39:16.47 delay 2 seconds @18:39:18.50 ARV.dial :air1 @18:39:18.89 delay 2 seconds @18:39:20.98 AS.reconfigure ASslow2 @18:39:21.03 AS.seek 0.506ml @18:39:27.30 delay 2 seconds @18:39:29.32 ARV.dial :MM1 @18:39:29.89 delay 2 seconds @18:39:31.92 Adding MM1 @18:39:31.99 AS.seek 0.524ml @18:39:50.21 delay 25 seconds @18:40:15.24 ARV.dial :PP1 @18:40:15.63 Adding PP1 @18:40:15.65 delay 2 seconds @18:40:17.73 AS.seek 0.53ml @18:40:23.96 delay 10 seconds @18:40:33.99 ARV.dial :air1 @18:40:34.38 adding ARV air @18:40:34.40 delay 2 seconds @18:40:36.48 AS.seek 0.536ml @18:40:42.74 delay 5 seconds @18:40:47.77 ARV.dial :carrier @18:40:48.14 delay 7 seconds @18:40:55.23 AS.seek 0.594ml @18:41:53.72 delay 5 seconds @18:41:58.75 ATV.dial :ASV @18:41:59.15 delay 2 seconds @18:42:01.24 AS.seek 0.6ml @18:42:07.63 delay 4 seconds @18:42:11.66 ATV.dial ARV @18:42:12.19 delay 2 seconds @18:42:14.22 ARV.dial :carrier @18:42:14.23 delay 2 seconds @18:42:16.32 AS.seek 0.65ml @18:43:07.02 delay 4 seconds @18:43:11.05 ATV.dial PCR @18:43:11.63 ASV.dial :waste1 @18:43:11.64 ARV.dial :air1 @18:43:12.03 Positioning Slug by 0.324ml @18:43:12.12 AS.seek 0.326ml @18:48:38.04 delay 5 seconds @18:48:43.07 ATV.dial ARV @18:48:43.81 ASV.dial :PCRlock @18:48:44.20 delay 5 seconds @18:48:49.23 Running NM Profile60C300 thermocycling @18:48:49.24 PCR.program Profile60C300 @18:48:49.35 Profile60C300 should complete in < 1:59:25 @18:48:50.02 PCR.run 2:34:16.25 @20:23:41.61 PCR.off @20:23:42.28 PCR for NM completed -- closed /var/log/esp/NM20may1818h400ml.pcr @20:23:42.35 Cleaning PCR @20:23:42.36 AFV.select :carrierSolution @20:23:42.47 delay 2 seconds @20:23:44.50 AS.reconfigure ASconfig @20:23:44.55 AS.seek 0.5ml @20:23:54.86 delay 1 second @20:23:55.88 AFV.select :holdingCoil @20:23:55.98 ASV.dial :waste1 @20:23:56.36 ATV.dial :air3 @20:23:57.58 AS.reconfigure ASslow1 @20:23:57.63 AS.seek 0.51ml @20:23:59.91 delay 2 seconds @20:24:01.93 ATV.dial :bleach @20:24:02.54 AS.seek 0.81ml @20:25:03.11 delay 3 seconds @20:25:06.14 ATV.dial :air3 @20:25:06.67 delay 3 seconds @20:25:09.76 AS.seek 0.835ml @20:25:15.04 delay 3 seconds @20:25:18.07 ATV.dial :bleach @20:25:18.68 AS.seek 0.82ml @20:25:21.97 delay 3 seconds @20:25:25.00 ARV.dial :waste @20:25:25.58 delay 3 seconds @20:25:28.61 ATV.dial :ARV @20:25:29.46 AS.reconfigure ASconfig @20:25:29.51 AS.seek 0.47ml @20:25:49.92 AFV.select :carrierSolution @20:25:50.03 delay 2 seconds @20:25:52.07 AS.seek 1ml @20:26:22.84 delay 1 second @20:26:23.86 AFV.select :holdingCoil @20:26:23.96 delay 1 second @20:26:24.99 AS.reconfigure ASfast @20:26:25.04 AS.seek 0ml @20:26:52.36 AFV.select :carrierSolution @20:26:52.47 delay 2 seconds @20:26:54.50 AS.reconfigure ASconfig @20:26:54.55 AS.seek 1ml @20:27:52.31 delay 1 second @20:27:53.33 AFV.select :holdingCoil @20:27:53.43 delay 1 second @20:27:54.46 AS.reconfigure ASfast @20:27:54.51 AS.seek 0ml @20:28:21.83 AFV.select :carrierSolution @20:28:21.94 delay 2 seconds @20:28:23.97 AS.reconfigure ASconfig @20:28:24.02 AS.seek 1ml @20:29:21.77 delay 1 second @20:29:22.79 AFV.select :holdingCoil @20:29:22.89 delay 1 second @20:29:23.92 AS.reconfigure ASfast @20:29:23.97 AS.seek 0ml @20:29:51.97 ARV.dial between carrier and air1 @20:29:52.45 ATV.dial :air3 @20:29:53.68 AS.reconfigure ASslow1 @20:29:53.73 AS.seek 0.01ml @20:29:56.11 delay 2 seconds @20:29:58.13 ATV.dial :bleach @20:29:58.74 AS.seek 0.81ml @20:32:39.86 delay 3 seconds @20:32:42.89 ATV.dial :air3 @20:32:43.43 delay 3 seconds @20:32:46.52 AS.seek 0.835ml @20:32:51.83 delay 3 seconds @20:32:54.86 ATV.dial :bleach @20:32:55.46 AS.seek 0.82ml @20:32:58.77 delay 3 seconds @20:33:01.80 ATV.dial PCR @20:33:02.19 AS.reconfigure ASconfig @20:33:02.24 AS.seek :empty @20:33:49.72 PCR.program :bleachProfile @20:33:49.78 PCR program should complete in < 2:45 @20:33:49.79 PCR.run 10:00 @20:37:03.73 PCR.off @20:37:03.76 AFV.select :carrierSolution @20:37:03.87 delay 2 seconds @20:37:05.91 AS.seek 1ml @20:38:03.73 delay 1 second @20:38:04.75 AFV.select :holdingCoil @20:38:04.85 delay 1 second @20:38:05.88 AS.reconfigure ASfast @20:38:05.93 AS.seek 0ml @20:38:33.27 delay 3 seconds @20:38:36.30 AFV.select :carrierSolution @20:38:36.40 delay 2 seconds @20:38:38.43 AS.reconfigure ASconfig @20:38:38.48 AS.seek 1ml @20:39:36.26 delay 1 second @20:39:37.28 AFV.select :holdingCoil @20:39:37.38 delay 1 second @20:39:38.41 AS.reconfigure ASfast @20:39:38.46 AS.seek 0ml @20:40:05.77 delay 3 seconds @20:40:08.80 AFV.select :carrierSolution @20:40:08.90 delay 2 seconds @20:40:10.93 AS.reconfigure ASconfig @20:40:10.99 AS.seek 1ml @20:41:08.74 delay 1 second @20:41:09.76 AFV.select :holdingCoil @20:41:09.86 delay 1 second @20:41:10.89 AS.reconfigure ASfast @20:41:10.94 AS.seek 0ml @20:41:38.29 delay 3 seconds @20:41:41.32 AFV.select :carrierSolution @20:41:41.42 delay 2 seconds @20:41:43.45 AS.reconfigure ASconfig @20:41:43.50 AS.seek 1ml @20:42:41.31 delay 1 second @20:42:42.33 AFV.select :holdingCoil @20:42:42.43 delay 1 second @20:42:43.46 AS.reconfigure ASfast @20:42:43.51 AS.seek 0ml @20:43:10.85 delay 3 seconds @20:43:13.88 AFV.select :carrierSolution @20:43:13.98 delay 2 seconds @20:43:16.01 AS.reconfigure ASconfig @20:43:16.07 AS.seek 1ml @20:44:13.79 delay 1 second @20:44:14.81 AFV.select :holdingCoil @20:44:14.91 delay 1 second @20:44:15.94 AS.reconfigure ASnorm @20:44:15.99 AS.seek 0ml @20:45:06.67 Setting up PCR for IPc -- writing /var/log/esp/IPc20may1820h400ml.pcr @20:45:06.68 Building PCR Slug from ASV with MM3 mix, PP6 primer @20:45:06.70 Priming with carrier @20:45:06.71 ATV.dial ARV @20:45:07.29 ARV.dial :carrier @20:45:07.63 AS.seek 0.5ml @20:45:33.26 AFV.select :carrierSolution @20:45:33.37 delay 2 seconds @20:45:35.40 AS.reconfigure ASconfig @20:45:35.45 AS.seek 1ml @20:46:04.47 delay 1 second @20:46:05.49 AFV.select :holdingCoil @20:46:05.59 delay 1 second @20:46:06.62 ATV.dial :waste @20:46:08.18 AS.reconfigure ASnorm @20:46:08.23 AS.seek :empty @20:46:59.00 AFV.select :carrierSolution @20:46:59.11 delay 2 seconds @20:47:01.14 AS.reconfigure ASconfig @20:47:01.19 AS.seek 0.5ml @20:47:30.31 delay 1 second @20:47:31.33 AFV.select :holdingCoil @20:47:31.43 Building Slug @20:47:31.44 ATV.dial :ARV @20:47:33.00 delay 2 seconds @20:47:35.67 ARV.dial :air1 @20:47:36.08 delay 2 seconds @20:47:38.17 AS.reconfigure ASslow2 @20:47:38.23 AS.seek 0.506ml @20:47:44.64 delay 2 seconds @20:47:46.67 ARV.dial :MM3 @20:47:47.67 delay 2 seconds @20:47:49.70 Adding MM3 @20:47:49.79 AS.seek 0.524ml @20:48:08.17 delay 25 seconds @20:48:33.20 ARV.dial :PP6 @20:48:33.58 Adding PP6 @20:48:33.60 delay 2 seconds @20:48:35.68 AS.seek 0.53ml @20:48:41.98 delay 10 seconds @20:48:52.01 ARV.dial :air1 @20:48:52.89 adding ARV air @20:48:52.91 delay 2 seconds @20:48:54.99 AS.seek 0.536ml @20:49:01.34 delay 5 seconds @20:49:06.37 ARV.dial :carrier @20:49:06.75 delay 7 seconds @20:49:13.84 AS.seek 0.594ml @20:50:12.41 delay 5 seconds @20:50:17.44 ATV.dial :ASV @20:50:17.83 delay 2 seconds @20:50:19.92 AS.seek 0.6ml @20:50:26.34 delay 4 seconds @20:50:30.37 ATV.dial ARV @20:50:30.89 delay 2 seconds @20:50:32.92 ARV.dial :carrier @20:50:32.93 delay 2 seconds @20:50:35.02 AS.seek 0.65ml @20:51:25.53 delay 4 seconds @20:51:29.56 ATV.dial PCR @20:51:30.14 ASV.dial :waste1 @20:51:30.15 ARV.dial :air1 @20:51:30.54 Positioning Slug by 0.324ml @20:51:30.63 AS.seek 0.326ml @20:56:56.47 delay 5 seconds @20:57:01.50 ATV.dial ARV @20:57:02.24 ASV.dial :PCRlock @20:57:02.63 delay 5 seconds @20:57:07.66 Running IPc ProfileIPc thermocycling @20:57:07.67 PCR.program ProfileIPc @20:57:07.78 ProfileIPc should complete in < 1:36:15 @20:57:07.79 PCR.run 2:05:18.75 @22:15:21.93 PCR.off @22:15:22.50 PCR for IPc completed -- closed /var/log/esp/IPc20may1820h400ml.pcr @22:15:22.57 Cleaning PCR @22:15:22.58 AFV.select :carrierSolution @22:15:22.69 delay 2 seconds @22:15:24.72 AS.reconfigure ASconfig @22:15:24.77 AS.seek 0.5ml @22:15:35.08 delay 1 second @22:15:36.10 AFV.select :holdingCoil @22:15:36.20 ASV.dial :waste1 @22:15:36.59 ATV.dial :air3 @22:15:37.82 AS.reconfigure ASslow1 @22:15:37.87 AS.seek 0.51ml @22:15:40.16 delay 2 seconds @22:15:42.19 ATV.dial :bleach @22:15:42.80 AS.seek 0.81ml @22:16:43.37 delay 3 seconds @22:16:46.40 ATV.dial :air3 @22:16:46.94 delay 3 seconds @22:16:50.03 AS.seek 0.835ml @22:16:55.32 delay 3 seconds @22:16:58.35 ATV.dial :bleach @22:16:58.96 AS.seek 0.82ml @22:17:02.25 delay 3 seconds @22:17:05.28 ARV.dial :waste @22:17:05.86 delay 3 seconds @22:17:08.89 ATV.dial :ARV @22:17:09.73 AS.reconfigure ASconfig @22:17:09.79 AS.seek 0.47ml @22:17:30.18 AFV.select :carrierSolution @22:17:30.30 delay 2 seconds @22:17:32.34 AS.seek 1ml @22:18:03.12 delay 1 second @22:18:04.14 AFV.select :holdingCoil @22:18:04.24 delay 1 second @22:18:05.27 AS.reconfigure ASfast @22:18:05.32 AS.seek 0ml @22:18:32.66 AFV.select :carrierSolution @22:18:32.77 delay 2 seconds @22:18:34.80 AS.reconfigure ASconfig @22:18:34.85 AS.seek 1ml @22:19:32.66 delay 1 second @22:19:33.68 AFV.select :holdingCoil @22:19:33.78 delay 1 second @22:19:34.81 AS.reconfigure ASfast @22:19:34.86 AS.seek 0ml @22:20:02.22 AFV.select :carrierSolution @22:20:02.33 delay 2 seconds @22:20:04.36 AS.reconfigure ASconfig @22:20:04.41 AS.seek 1ml @22:21:02.20 delay 1 second @22:21:03.22 AFV.select :holdingCoil @22:21:03.32 delay 1 second @22:21:04.35 AS.reconfigure ASfast @22:21:04.40 AS.seek 0ml @22:21:31.75 ARV.dial between carrier and air1 @22:21:32.24 ATV.dial :air3 @22:21:33.46 AS.reconfigure ASslow1 @22:21:33.51 AS.seek 0.01ml @22:21:35.85 delay 2 seconds @22:21:37.87 ATV.dial :bleach @22:21:38.48 AS.seek 0.81ml @22:24:19.61 delay 3 seconds @22:24:22.64 ATV.dial :air3 @22:24:23.18 delay 3 seconds @22:24:26.27 AS.seek 0.835ml @22:24:31.55 delay 3 seconds @22:24:34.57 ATV.dial :bleach @22:24:35.18 AS.seek 0.82ml @22:24:38.47 delay 3 seconds @22:24:41.50 ATV.dial PCR @22:24:41.89 AS.reconfigure ASconfig @22:24:41.94 AS.seek :empty @22:25:30.07 PCR.program :bleachProfile @22:25:30.13 PCR program should complete in < 2:45 @22:25:30.14 PCR.run 10:00 @22:28:44.08 PCR.off @22:28:44.10 AFV.select :carrierSolution @22:28:44.21 delay 2 seconds @22:28:46.25 AS.seek 1ml @22:29:44.04 delay 1 second @22:29:45.06 AFV.select :holdingCoil @22:29:45.16 delay 1 second @22:29:46.19 AS.reconfigure ASfast @22:29:46.24 AS.seek 0ml @22:30:13.59 delay 3 seconds @22:30:16.62 AFV.select :carrierSolution @22:30:16.72 delay 2 seconds @22:30:18.75 AS.reconfigure ASconfig @22:30:18.81 AS.seek 1ml @22:31:16.63 delay 1 second @22:31:17.65 AFV.select :holdingCoil @22:31:17.75 delay 1 second @22:31:18.78 AS.reconfigure ASfast @22:31:18.83 AS.seek 0ml @22:31:46.16 delay 3 seconds @22:31:49.19 AFV.select :carrierSolution @22:31:49.29 delay 2 seconds @22:31:51.32 AS.reconfigure ASconfig @22:31:51.38 AS.seek 1ml @22:32:49.18 delay 1 second @22:32:50.20 AFV.select :holdingCoil @22:32:50.30 delay 1 second @22:32:51.33 AS.reconfigure ASfast @22:32:51.38 AS.seek 0ml @22:33:18.74 delay 3 seconds @22:33:21.77 AFV.select :carrierSolution @22:33:21.87 delay 2 seconds @22:33:23.90 AS.reconfigure ASconfig @22:33:23.96 AS.seek 1ml @22:34:21.81 delay 1 second @22:34:22.83 AFV.select :holdingCoil @22:34:22.93 delay 1 second @22:34:23.96 AS.reconfigure ASfast @22:34:24.01 AS.seek 0ml @22:34:51.35 delay 3 seconds @22:34:54.38 AFV.select :carrierSolution @22:34:54.49 delay 2 seconds @22:34:56.51 AS.reconfigure ASconfig @22:34:56.56 AS.seek 1ml @22:35:54.37 delay 1 second @22:35:55.39 AFV.select :holdingCoil @22:35:55.49 delay 1 second @22:35:56.52 AS.reconfigure ASnorm @22:35:56.57 AS.seek 0ml @22:36:47.33 Setting up PCR for IPc2 -- writing /var/log/esp/IPc220may1822h400ml.pcr @22:36:47.35 Building PCR Slug from ASV with MM2 mix, PP3 primer @22:36:47.36 Priming with carrier @22:36:47.37 ATV.dial ARV @22:36:47.95 ARV.dial :carrier @22:36:48.30 AS.seek 0.5ml @22:37:13.87 AFV.select :carrierSolution @22:37:13.98 delay 2 seconds @22:37:16.01 AS.reconfigure ASconfig @22:37:16.06 AS.seek 1ml @22:37:45.14 delay 1 second @22:37:46.16 AFV.select :holdingCoil @22:37:46.26 delay 1 second @22:37:47.29 ATV.dial :waste @22:37:48.86 AS.reconfigure ASnorm @22:37:48.91 AS.seek :empty @22:38:39.67 AFV.select :carrierSolution @22:38:39.78 delay 2 seconds @22:38:41.81 AS.reconfigure ASconfig @22:38:41.86 AS.seek 0.5ml @22:39:10.94 delay 1 second @22:39:11.96 AFV.select :holdingCoil @22:39:12.06 Building Slug @22:39:12.07 ATV.dial :ARV @22:39:13.62 delay 2 seconds @22:39:15.65 ARV.dial :air1 @22:39:16.04 delay 2 seconds @22:39:18.12 AS.reconfigure ASslow2 @22:39:18.18 AS.seek 0.506ml @22:39:24.34 delay 2 seconds @22:39:26.36 ARV.dial :MM2 @22:39:27.76 delay 2 seconds @22:39:29.79 Adding MM2 @22:39:29.86 AS.seek 0.524ml @22:39:48.23 delay 25 seconds @22:40:13.26 ARV.dial :PP3 @22:40:13.64 Adding PP3 @22:40:13.65 delay 2 seconds @22:40:15.74 AS.seek 0.53ml @22:40:22.09 delay 10 seconds @22:40:32.12 ARV.dial :air2 @22:40:32.50 adding ARV air @22:40:32.51 delay 2 seconds @22:40:34.60 AS.seek 0.536ml @22:40:40.91 delay 5 seconds @22:40:45.94 ARV.dial :carrier @22:40:47.78 delay 7 seconds @22:40:54.87 AS.seek 0.594ml @22:41:53.70 delay 5 seconds @22:41:58.73 ATV.dial :ASV @22:41:59.13 delay 2 seconds @22:42:01.22 AS.seek 0.6ml @22:42:07.66 delay 4 seconds @22:42:11.69 ATV.dial ARV @22:42:12.22 delay 2 seconds @22:42:14.25 ARV.dial :carrier @22:42:14.26 delay 2 seconds @22:42:16.35 AS.seek 0.65ml @22:43:06.86 delay 4 seconds @22:43:10.89 ATV.dial PCR @22:43:11.47 ASV.dial :waste1 @22:43:11.48 ARV.dial :air1 @22:43:11.89 Positioning Slug by 0.324ml @22:43:11.98 AS.seek 0.326ml @22:48:38.27 delay 5 seconds @22:48:43.30 ATV.dial ARV @22:48:44.04 ASV.dial :PCRlock @22:48:44.43 delay 5 seconds @22:48:49.46 Running IPc2 ProfileIPc thermocycling @22:48:49.47 PCR.program ProfileIPc @22:48:49.58 ProfileIPc should complete in < 1:36:15 @22:48:49.59 PCR.run 2:05:18.75 @00:06:49.74CEST19-May-20 PCR.off @00:06:50.95 PCR for IPc2 completed -- closed /var/log/esp/IPc220may1822h400ml.pcr @00:06:50.97 Full MFB Decontamination @00:06:50.98 Decon extraction column and decon AIV @00:06:51.00 SPE.hold 2:46:40,55C @00:06:51.05 ASV.dial :SPEdelivery @00:06:51.69 delay 20 seconds @00:07:11.72 AFV.select :carrierSolution @00:07:11.82 delay 2 seconds @00:07:13.85 AS.reconfigure ASconfig @00:07:13.90 AS.seek 0.5ml @00:07:24.20 delay 1 second @00:07:25.22 AFV.select :holdingCoil @00:07:25.32 ATV.dial :air1 @00:07:27.27 AS.reconfigure ASslow1 @00:07:27.32 AS.seek 0.52ml @00:07:31.62 ATV.dial :ASV @00:07:33.12 ASV.dial :SPErecovery @00:07:33.77 AS.seek 0.62ml @00:07:54.15 ATV.dial :waste @00:07:56.03 AS.reconfigure ASnorm @00:07:56.08 AS.seek :empty @00:08:27.69 AFV.select :holdingCoil @00:08:27.70 ATV.dial :waste @00:08:27.72 AS.reconfigure ASconfig @00:08:27.77 AS.seek 0ml @00:08:28.06 AFV.select :carrierSolution @00:08:28.17 delay 2 seconds @00:08:30.21 AS.seek 0.5ml @00:08:59.23 delay 1 second @00:09:00.25 AFV.select :holdingCoil @00:09:00.35 ATV.dial :air3 @00:09:01.05 AS.reconfigure ASslow1 @00:09:01.10 AS.seek 0.52ml @00:09:05.37 ATV.dial :air3 @00:09:05.46 AS.seek 0.53ml @00:09:07.75 delay 2 seconds @00:09:09.77 ATV.dial :bleach @00:09:10.24 AS.seek 0.71ml @00:09:46.70 delay 3 seconds @00:09:49.73 ATV.dial :air3 @00:09:50.28 delay 3 seconds @00:09:53.37 AS.seek 0.735ml @00:09:58.66 delay 3 seconds @00:10:01.69 ATV.dial :bleach @00:10:02.30 AS.seek 0.72ml @00:10:05.69 delay 3 seconds @00:10:08.72 ATV.dial ASV @00:10:09.75 ASV.dial :SPErecovery @00:10:09.84 AS.seek 0.365ml @00:11:21.50 ATV.dial :waste @00:11:23.13 AS.reconfigure ASnorm @00:11:23.18 AS.seek :empty @00:11:41.97 ATV.dial :air3 @00:11:42.63 AS.reconfigure ASslow1 @00:11:42.68 AS.seek 0.009ml @00:11:44.97 delay 2 seconds @00:11:46.99 ATV.dial :bleach @00:11:47.45 AS.seek 0.579ml @00:13:42.33 delay 3 seconds @00:13:45.36 ATV.dial :air3 @00:13:45.89 delay 3 seconds @00:13:48.98 AS.seek 0.604ml @00:13:54.28 delay 3 seconds @00:13:57.31 ATV.dial :bleach @00:13:57.92 AS.seek 0.589ml @00:14:01.19 delay 3 seconds @00:14:04.21 ATV.dial :ASV @00:14:05.21 ASV.dial :wasteR @00:14:05.68 AS.seek 0ml @00:16:04.44 delay 1:00 @00:17:04.47 ATV.dial ASV @00:17:04.48 AFV.select :carrierSolution @00:17:04.58 delay 2 seconds @00:17:06.61 AS.reconfigure ASconfig @00:17:06.66 AS.seek 1ml @00:18:04.46 delay 1 second @00:18:05.48 AFV.select :holdingCoil @00:18:05.58 delay 1 second @00:18:06.61 ASV.dial :wasteR @00:18:06.63 AS.reconfigure ASslow1 @00:18:06.67 AS.seek 0ml @00:21:28.04 delay 10 seconds @00:21:38.07 ATV.dial :air3 @00:21:39.50 AS.seek 0.01ml @00:21:41.79 delay 2 seconds @00:21:43.81 ATV.dial :bleach @00:21:45.07 AS.seek 0.58ml @00:23:39.95 delay 3 seconds @00:23:42.98 ATV.dial :air3 @00:23:43.51 delay 3 seconds @00:23:46.60 AS.seek 0.605ml @00:23:51.96 delay 3 seconds @00:23:54.99 ATV.dial :bleach @00:23:55.60 AS.seek 0.59ml @00:23:58.90 delay 3 seconds @00:24:01.93 ATV.dial ASV @00:24:02.92 ASV.dial :SPEdelivery @00:24:03.30 AS.seek 0ml @00:26:02.20 delay 1:00 @00:27:02.23 AFV.select :carrierSolution @00:27:02.33 delay 2 seconds @00:27:04.36 AS.reconfigure ASconfig @00:27:04.41 AS.seek 1ml @00:28:02.17 delay 1 second @00:28:03.19 AFV.select :holdingCoil @00:28:03.29 delay 1 second @00:28:04.32 ATV.dial ASV @00:28:04.33 ASV.dial :SPEdelivery @00:28:04.35 AS.reconfigure ASslow1 @00:28:04.39 AS.seek 0ml @00:31:25.80 delay 10 seconds @00:31:35.83 ATV.dial :air2 @00:31:36.63 AS.reconfigure ASslow0 @00:31:36.68 AS.seek 0.8ml @00:32:57.45 delay 3 seconds @00:33:00.48 ATV.dial :ASV @00:33:01.42 ASV.dial :SPEdelivery @00:33:01.44 AS.reconfigure ASslow1 @00:33:01.49 AS.seek 0.03ml @00:35:36.61 delay 20 seconds @00:35:56.64 ATV.dial :waste,via: :air3 @00:35:58.25 AS.reconfigure ASconfig @00:35:58.30 AS.seek :empty @00:36:00.35 SPE.coast @00:36:00.44 Removing Bleach from Mixing Coil @00:36:00.46 AFV.select :carrierSolution @00:36:00.56 delay 2 seconds @00:36:02.60 AS.seek 0.5ml @00:36:31.70 delay 1 second @00:36:32.72 AFV.select :holdingCoil @00:36:32.82 ATV.dial :ASV @00:36:34.42 ASV.dial :SPErecovery @00:36:35.06 AS.reconfigure ASslow1 @00:36:35.11 AS.seek 0.73ml @00:37:21.64 ASV.dial :wasteD @00:37:22.19 AS.reconfigure ASnorm @00:37:22.24 AS.seek 0ml @00:37:59.38 AFV.select :carrierSolution @00:37:59.49 delay 2 seconds @00:38:01.52 AS.reconfigure ASconfig @00:38:01.57 AS.seek 0.5ml @00:38:30.60 delay 1 second @00:38:31.62 AFV.select :holdingCoil @00:38:31.72 ATV.dial ASV @00:38:31.73 ASV.dial :SPErecovery @00:38:32.37 AS.reconfigure ASslow0 @00:38:32.42 AS.seek 0.3ml @00:38:52.83 ATV.dial :air2 @00:38:53.82 AS.reconfigure ASslow1 @00:38:53.87 AS.seek 0.32ml @00:38:58.25 ATV.dial ASV @00:38:59.25 AS.reconfigure ASslow0 @00:38:59.30 AS.seek 0.57ml @00:39:24.83 ASV.dial :wasteD @00:39:25.39 AS.reconfigure ASnorm @00:39:25.44 AS.seek 0ml @00:39:54.53 AFV.select :carrierSolution @00:39:54.64 delay 2 seconds @00:39:56.67 AS.reconfigure ASconfig @00:39:56.72 AS.seek 0.5ml @00:40:25.75 delay 1 second @00:40:26.77 AFV.select :holdingCoil @00:40:26.87 ATV.dial ASV @00:40:26.88 ASV.dial :SPErecovery @00:40:27.53 AS.reconfigure ASslow0 @00:40:27.58 AS.seek 0.3ml @00:40:48.02 ATV.dial :air2 @00:40:49.03 AS.reconfigure ASslow1 @00:40:49.08 AS.seek 0.32ml @00:40:53.40 ATV.dial ASV @00:40:54.40 AS.reconfigure ASslow0 @00:40:54.45 AS.seek 0.57ml @00:41:19.91 ASV.dial :wasteD @00:41:20.47 AS.reconfigure ASnorm @00:41:20.52 AS.seek 0ml @00:41:49.63 ATV.dial :air2 @00:41:51.22 AS.reconfigure ASslow0 @00:41:51.26 AS.seek 0.3ml @00:42:21.77 ATV.dial :ASV @00:42:22.71 ASV.dial :wasteD @00:42:22.73 AS.reconfigure ASconfig @00:42:22.78 AS.seek 0.05ml @00:42:37.47 ATV.dial :waste @00:42:39.23 AS.seek 0ml @00:42:42.41 ASV.dial :SPEdelivery @00:42:43.37 deconLysateINT @00:42:43.38 ATV.dial :air3 @00:42:44.06 AS.reconfigure ASslow1 @00:42:44.11 AS.seek 0.01ml @00:42:46.41 delay 2 seconds @00:42:48.43 ATV.dial :bleach @00:42:48.89 AS.seek 0.96ml @00:46:00.24 delay 3 seconds @00:46:03.27 ATV.dial :air3 @00:46:03.80 delay 3 seconds @00:46:06.89 AS.seek 0.985ml @00:46:12.24 delay 3 seconds @00:46:15.27 ATV.dial :bleach @00:46:15.87 AS.seek 0.97ml @00:46:19.15 delay 3 seconds @00:46:22.18 ATV.dial AIV @00:46:23.37 AIV.dial :coilToMFB @00:46:23.39 AWV.select :waste @00:46:23.40 AFV.select :carrierSolution @00:46:23.51 delay 2 seconds @00:46:25.54 AS.reconfigure ASconfig @00:46:25.59 AS.seek 1ml @00:46:27.65 delay 1 second @00:46:28.67 AFV.select :holdingCoil @00:46:28.77 delay 1 second @00:46:29.80 AS.reconfigure ASslow0 @00:46:29.85 AS.seek :empty @00:48:10.84 AFV.select :carrierSolution @00:48:10.95 delay 2 seconds @00:48:12.98 AS.reconfigure ASconfig @00:48:13.03 AS.seek 1ml @00:49:10.87 delay 1 second @00:49:11.89 AFV.select :holdingCoil @00:49:11.99 delay 1 second @00:49:13.02 AS.reconfigure ASslow0 @00:49:13.07 AS.seek :empty @00:50:54.01 AFV.select :carrierSolution @00:50:54.12 delay 2 seconds @00:50:56.15 AS.reconfigure ASconfig @00:50:56.20 AS.seek 1ml @00:51:54.07 delay 1 second @00:51:55.09 AFV.select :holdingCoil @00:51:55.19 delay 1 second @00:51:56.22 AS.reconfigure ASslow0 @00:51:56.27 AS.seek :empty @00:53:37.28 ATV.dial :air2 @00:53:37.94 AS.reconfigure ASslow1 @00:53:37.99 AS.seek 0.009ml @00:53:40.28 AS.reconfigure ASslow0 @00:53:40.33 AS.seek 1ml @00:55:20.26 ATV.dial AIV @00:55:21.01 AS.seek 0.02ml @00:56:59.90 ATV.dial :waste @00:57:01.46 AS.seek 0ml @00:57:03.76 AWV.select :air @00:57:03.93 Cleaning PCR @00:57:03.94 AFV.select :carrierSolution @00:57:04.05 delay 2 seconds @00:57:06.08 AS.reconfigure ASconfig @00:57:06.13 AS.seek 0.5ml @00:57:35.16 delay 1 second @00:57:36.18 AFV.select :holdingCoil @00:57:36.28 ASV.dial :waste1 @00:57:36.66 ATV.dial :air3 @00:57:37.33 AS.reconfigure ASslow1 @00:57:37.38 AS.seek 0.51ml @00:57:39.67 delay 2 seconds @00:57:41.69 ATV.dial :bleach @00:57:42.15 AS.seek 0.81ml @00:58:42.72 delay 3 seconds @00:58:45.75 ATV.dial :air3 @00:58:46.28 delay 3 seconds @00:58:49.37 AS.seek 0.835ml @00:58:54.66 delay 3 seconds @00:58:57.69 ATV.dial :bleach @00:58:58.30 AS.seek 0.82ml @00:59:01.56 delay 3 seconds @00:59:04.59 ARV.dial :waste @00:59:05.17 delay 3 seconds @00:59:08.20 ATV.dial :ARV @00:59:09.05 AS.reconfigure ASconfig @00:59:09.74 AS.seek 0.47ml @00:59:30.16 AFV.select :carrierSolution @00:59:30.27 delay 2 seconds @00:59:32.31 AS.seek 1ml @01:00:03.05 delay 1 second @01:00:04.07 AFV.select :holdingCoil @01:00:04.17 delay 1 second @01:00:05.20 AS.reconfigure ASfast @01:00:05.25 AS.seek 0ml @01:00:32.59 AFV.select :carrierSolution @01:00:32.70 delay 2 seconds @01:00:34.73 AS.reconfigure ASconfig @01:00:34.78 AS.seek 1ml @01:01:32.60 delay 1 second @01:01:33.62 AFV.select :holdingCoil @01:01:33.72 delay 1 second @01:01:34.75 AS.reconfigure ASfast @01:01:34.80 AS.seek 0ml @01:02:02.14 AFV.select :carrierSolution @01:02:02.25 delay 2 seconds @01:02:04.28 AS.reconfigure ASconfig @01:02:04.33 AS.seek 1ml @01:03:02.16 delay 1 second @01:03:03.18 AFV.select :holdingCoil @01:03:03.28 delay 1 second @01:03:04.31 AS.reconfigure ASfast @01:03:04.36 AS.seek 0ml @01:03:31.72 ARV.dial between carrier and air1 @01:03:32.21 ATV.dial :air3 @01:03:33.45 AS.reconfigure ASslow1 @01:03:33.50 AS.seek 0.01ml @01:03:35.78 delay 2 seconds @01:03:37.80 ATV.dial :bleach @01:03:38.40 AS.seek 0.81ml @01:06:19.52 delay 3 seconds @01:06:22.55 ATV.dial :air3 @01:06:23.08 delay 3 seconds @01:06:26.17 AS.seek 0.835ml @01:06:31.55 delay 3 seconds @01:06:34.58 ATV.dial :bleach @01:06:35.18 AS.seek 0.82ml @01:06:38.46 delay 3 seconds @01:06:41.49 ATV.dial PCR @01:06:41.88 AS.reconfigure ASconfig @01:06:41.93 AS.seek :empty @01:07:29.40 PCR.program :bleachProfile @01:07:29.46 PCR program should complete in < 2:45 @01:07:29.47 PCR.run 10:00 @01:10:58.56 PCR.off @01:10:58.59 AFV.select :carrierSolution @01:10:58.70 delay 2 seconds @01:11:00.74 AS.seek 1ml @01:11:58.55 delay 1 second @01:11:59.57 AFV.select :holdingCoil @01:11:59.67 delay 1 second @01:12:00.70 AS.reconfigure ASfast @01:12:00.75 AS.seek 0ml @01:12:28.07 delay 3 seconds @01:12:31.10 AFV.select :carrierSolution @01:12:31.20 delay 2 seconds @01:12:33.23 AS.reconfigure ASconfig @01:12:33.29 AS.seek 1ml @01:13:31.09 delay 1 second @01:13:32.11 AFV.select :holdingCoil @01:13:32.21 delay 1 second @01:13:33.24 AS.reconfigure ASfast @01:13:33.29 AS.seek 0ml @01:14:00.63 delay 3 seconds @01:14:03.66 AFV.select :carrierSolution @01:14:03.76 delay 2 seconds @01:14:05.79 AS.reconfigure ASconfig @01:14:05.84 AS.seek 1ml @01:15:03.62 delay 1 second @01:15:04.64 AFV.select :holdingCoil @01:15:04.74 delay 1 second @01:15:05.77 AS.reconfigure ASfast @01:15:05.82 AS.seek 0ml @01:15:33.17 delay 3 seconds @01:15:36.20 AFV.select :carrierSolution @01:15:36.30 delay 2 seconds @01:15:38.33 AS.reconfigure ASconfig @01:15:38.39 AS.seek 1ml @01:16:36.17 delay 1 second @01:16:37.19 AFV.select :holdingCoil @01:16:37.29 delay 1 second @01:16:38.32 AS.reconfigure ASfast @01:16:38.37 AS.seek 0ml @01:17:05.71 delay 3 seconds @01:17:08.74 AFV.select :carrierSolution @01:17:08.84 delay 2 seconds @01:17:10.87 AS.reconfigure ASconfig @01:17:10.93 AS.seek 1ml @01:18:08.72 delay 1 second @01:18:09.74 AFV.select :holdingCoil @01:18:09.84 delay 1 second @01:18:10.87 AS.reconfigure ASnorm @01:18:10.92 AS.seek 0ml @01:19:01.65 AFV.select :carrierSolution @01:19:01.76 delay 2 seconds @01:19:04.35 AS.reconfigure ASconfig @01:19:04.48 AS.seek 1ml @01:20:02.28 delay 1 second @01:20:03.30 AFV.select :holdingCoil @01:20:03.40 delay 1 second @01:20:04.43 ATV.dial :waste @01:20:05.60 AS.reconfigure ASnorm @01:20:05.65 AS.seek 0.5ml @01:20:31.22 ATV.dial :waste2 @01:20:32.45 AS.seek 0ml @01:20:58.02 AS.coast @01:20:58.06 SS.coast @01:20:58.10 CC.coast @01:20:58.14 CS.coast @01:20:58.18 PC.coast @01:20:58.22 PS.coast @01:20:58.26 Forearm.coast @01:20:58.30 Elbow.coast @01:20:58.70 Gate.power :core,:OFF @01:20:58.72 Gate.power :raw,:OFF Shutdown @01:20:58.74 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @10:01:37.93
Powering core bus @10:01:42.74 Gate.power :raw,:ON @10:01:43.40 SPE.coast @10:01:43.46 SPE.configure SPEconfig @10:01:43.55 AS.configure ASconfig @10:01:43.65 SS.configure SSconfig @10:01:43.75 CC.configure CCconfig @10:01:43.85 CS.configure CSconfig @10:01:43.95 PC.configure PCconfig @10:01:44.04 PS.configure PSconfig @10:01:44.16 Forearm.configure LooseForearm @10:01:44.26 Elbow.configure LooseElbow @10:01:44.38 SC.configure SCloose @10:01:44.48 SE.configure SEconfig @10:01:44.52 CH.coast @10:01:44.58 CH.configure CHconfig @10:01:44.61 PH.coast @10:01:44.67 PH.configure PHconfig @10:01:44.71 PCR.off @10:01:44.74 PCR.pidtemp= 50,0.1,0,300,25,25 @10:01:44.78 PCR.caltemp= 2.8814,0.0372,0 @10:01:44.81 All dwarf controllers running firmware version 3.75 @10:01:44.92 Valve.reset @10:01:45.09 PCR.off @10:01:45.12 PCR.pidtemp= 50,0.1,0,300,25,25 @10:01:45.15 PCR.caltemp= 2.8814,0.0372,0 @10:01:45.37 Intake.select :close @10:01:45.39 Exhaust.select :close @10:01:45.40 CTV.dial :air @10:01:45.50 PSV.dial :air @10:01:45.52 CSV.dial :waste1 @10:01:45.69
Forearm.reconfigure LooseForearm @10:01:45.75 Forearm.home.seek :retract @10:01:45.78 SSV.dial :spit,avoiding: :exhaust @10:01:46.79 Hand.close @10:01:46.85 CS.reconfigure CSfast @10:01:46.91 PS.reconfigure PSconfig @10:01:46.96 SS.reconfigure SSconfig @10:01:47.00
Elbow.jog 1500 @10:01:47.14 CS.home.seek 0ml @10:01:47.25 PS.home.seek 0ml @10:01:47.28 SS.home.seek 0ml @10:01:47.83
Elbow.reconfigure LooseElbow @10:01:47.88 Elbow.home.seek :clear @10:01:51.17 SE.reconfigure SEconfig @10:01:51.22 SE.home.seek :down @10:01:51.86 SC.reconfigure SCsloppy @10:01:51.91 SC.home.seek :initial @10:01:54.15 Forearm.seek :retract @10:01:54.23 Elbow.seek :hammer @10:01:55.43 Forearm.reconfigure TightForearm @10:01:55.47 Forearm.seek 5 + 350 counts @10:01:56.37 PS.coast @10:01:56.59
Forearm.seek :retract @10:01:57.73 PC.reconfigure PCconfig @10:01:57.74
CC.reconfigure CCconfig @10:01:57.84 PC.home.seek :open @10:01:57.88
CC.home.seek :open @10:02:01.30 Phase [stdphase] @10:02:01.31 GoodNews.email "At 10:02:01 began in tube 2: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:124\n{1 => 18, 2 => 4}\nCan@10:02:01, 8.4C, 44% humidity, 14.3psia, 13.648V, 0.815A, 0.815A avg, 11.12W",Subject: "Started 20invasivedeployfinal phase stdphase" @10:02:01.35CEST19-May-20
===> STARTED Superphase (phase #6) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @10:02:01.37 Starting initialPurge @10:02:01.39 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:02:01.46 Hand.open @10:02:02.21 Elbow.reconfigure TightElbow @10:02:02.26 Elbow.seek FlushPuck::Garage @10:02:04.28 Elbow.reconfigure LooseElbow @10:02:04.40 Forearm.reconfigure LooseForearm @10:02:04.46 Forearm.seek :garage @10:02:05.45 Hand.close @10:02:06.44 Forearm.seek :retract @10:02:07.44 Elbow.reconfigure TightElbow @10:02:07.49 Elbow.seek CC @10:02:10.61 Elbow.reconfigure LooseElbow @10:02:10.87 Forearm.reconfigure TightForearm @10:02:10.96 Forearm.seek CC @10:02:12.61 CC.seek :closed @10:02:17.10 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.02 @10:02:17.33 Hand.open @10:02:18.15 Forearm.reconfigure LooseForearm @10:02:18.24 Forearm.seek :retract @10:02:19.27 Flushing the Collection Valve::Manifold @10:02:19.55 CSV.dial CSR @10:02:21.07 Pulling 5ml of CSR.flush @10:02:21.08 CSR.select :flush @10:02:21.29 CS.reconfigure CSconfig @10:02:21.40 CS.seek 5ml @10:02:35.25 delay 5 seconds @10:02:40.28 CSR.select :air @10:02:40.54 CS.reconfigure CSfast @10:02:40.67 CS.seek 7.5ml @10:02:47.40 CSV.dial :waste @10:02:48.80 CS.reconfigure CSconfig @10:02:48.90 CS.seek 0ml @10:03:09.28 Purging external Intake @10:03:09.44 CSV.dial :flush @10:03:09.92 CS.reconfigure CSfast @10:03:09.97 CS.seek 10ml @10:03:33.53 delay 5 seconds @10:03:38.55 CSV.dial CBV @10:03:38.58
CBV.dial CSV @10:03:39.39 CTV.dial :intake @10:03:40.17 Intake.select :open @10:03:40.29 CS.seek 0ml @10:04:05.22 Intake.select :close @10:04:05.33 CTV.dial :air @10:04:06.11 Flushing upper manifold @10:04:06.24 CSV.dial :flush @10:04:07.58 CS.seek 5ml @10:04:19.91 delay 5 seconds @10:04:24.94 CSV.dial :air @10:04:25.48 CS.seek 7ml @10:04:31.03 CSV.dial CBV @10:04:31.06
CBV.dial CSV @10:04:32.14 CTV.dial :air @10:04:32.16 CS.seek 0ml @10:04:49.19 delay 7 seconds @10:04:56.23 CS.seek 3ml @10:05:04.00 CSV.dial :waste @10:05:04.48 CS.seek 0ml @10:05:12.72 CSV.dial :flush @10:05:13.19 CS.seek 3ml @10:05:21.00 delay 5 seconds @10:05:26.03 CSV.dial :air @10:05:26.57 CS.seek 5ml @10:05:32.13 CSV.dial :air @10:05:32.15 CS.seek 0ml @10:05:44.38 delay 7 seconds @10:05:51.42 CS.seek 3ml @10:05:59.16 CSV.dial :waste @10:05:59.94 CS.seek 0ml @10:06:07.79 Garaging FlushPuck in Collection Clamp @10:06:07.87 Elbow.reconfigure TightElbow @10:06:07.92 Elbow.seek CC @10:06:08.19 Elbow.reconfigure LooseElbow @10:06:08.30 Forearm.seek CC @10:06:09.29 Hand.close @10:06:09.96 CC.seek :open @10:06:14.32 Forearm.seek :retract @10:06:15.29 Elbow.reconfigure TightElbow @10:06:15.34 Elbow.seek :garage @10:06:18.62 Elbow.reconfigure LooseElbow @10:06:18.72 Forearm.reconfigure TightForearm @10:06:18.77 Forearm.seek :garage @10:06:19.92 Hand.open @10:06:20.66 Forearm.reconfigure LooseForearm @10:06:20.70 Forearm.seek :retract @10:06:21.80 Starting mfb @10:06:21.81 pcrBAC sampling at most 1500ml, wcr at most 1500ml @10:06:21.89 BACfans.email "Started mfb in tube 2 at 10:06:21CEST19-May-20\n{1 => 18, 2 => 4}\nCan@10:04:48, 8.4C, 44% humidity, 14.3psia, 13.652V, 0.733A, 0.733A avg, 10.01W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @10:06:21.94
Loading sh1 puck from tube 2 into Collection Clamp @10:06:22.05 Readying the MFB @10:06:22.09 Homing MFB Syringe @10:06:22.13 AFV.select :holdingCoil @10:06:22.37 ATV.dial :air2 @10:06:22.53
SC.reconfigure SCloose @10:06:22.78 Elbow.reconfigure TightElbow @10:06:22.83 Elbow.seek 2 @10:06:23.00 AS.reconfigure ASconfig @10:06:23.05 AS.home.seek :home @10:06:24.98
Forearm.reconfigure TightForearm @10:06:25.03 Forearm.seek 2 + 700 counts @10:06:26.43 SE.reconfigure SEpick @10:06:26.50 SE.seek beamBreak @10:06:28.57 AFV.select :carrierSolution @10:06:28.68 delay 2 seconds @10:06:29.40
SE.configure SEconfig @10:06:29.82 Elbow.seek 2 @10:06:30.29 Forearm.seek 2 + 300 counts @10:06:30.61 Forearm.reconfigure LooseForearm @10:06:30.71 Hand.close @10:06:30.84 AS.seek 1ml @10:06:31.40 SE.seek :down @10:06:31.98
Forearm.seek :retract @10:06:32.98 Elbow.seek CC @10:06:34.25 SE.coast @10:06:34.86
Elbow.reconfigure LooseElbow @10:06:35.16 Forearm.reconfigure TightForearm @10:06:35.29 Forearm.seek CC @10:06:36.96 CC.seek :closed @10:06:41.38 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.01 @10:06:41.42 Hand.open @10:06:41.60 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[0F:27->20] @10:06:42.25 Forearm.reconfigure LooseForearm @10:06:42.34 Forearm.seek :retract @10:06:43.39 SC.coast @10:06:43.47 collectSample 1500ml w/bubble point of 55psi @10:06:43.49 Priming 7ml @10:06:43.51 Intake.select :close @10:06:43.54 Exhaust.select :close @10:06:43.57 SS.hold @10:06:44.07 Calibrating pressure and temperature sensors @10:06:44.10 CTV.dial :air @10:06:44.13 SSV.dial :spit,avoiding: :exhaust @10:06:44.21 SS.reconfigure SSfast @10:06:44.31 SS.seek 5ml @10:06:47.00 SSV.dial CBV @10:06:47.08
CBV.dial SSV @10:06:48.56 delay 3.8 seconds @10:06:52.69 SSV.dial :spit @10:06:54.13 SS.seek 0ml @10:06:56.81 SSV.dial CBV @10:06:58.23 CTV.dial :intake @10:06:59.14 SSV.dial CBV @10:06:59.20
CBV.dial SSV @10:06:59.23 Allowing 40s for 7.0ml of remaining 7.0ml @10:06:59.25 Intake.select :open @10:07:01.56 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:07:01.59 SS.reconfigure SSinhale.with maxSpeed: 150 @10:07:01.69 SS.seek 7ml @10:07:05.33 Vacuum too high -- waiting up to 40s @10:07:07.89 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:07:07.92 SS.reconfigure SSinhale.with maxSpeed: 135 @10:07:08.02 SS.seek 7ml @10:07:11.26 Vacuum too high -- waiting up to 40s @10:07:13.42 SS.reconfigure SSinhale.with maxSpeed: 122 @10:07:13.52 SS.seek 7ml @10:07:13.81 Stroke completed -- waiting for pressure to equalize ... @10:07:17.04 Intake.select :close @10:07:17.30 SSV.dial :exhaust @10:07:18.15 Exhaust.select :open @10:07:18.32 SS.reconfigure SSexhale @10:07:18.42 SS.seek 0ml @10:07:22.32 Exhaust.select :close @10:07:22.48 Sampling 1500.0ml @10:07:22.51 Intake.select :close @10:07:22.53 Exhaust.select :close @10:07:22.56 SS.reconfigure SSconfig @10:07:22.65 SS.hold @10:07:23.10 CTV.dial :intake @10:07:23.13 SSV.dial CBV @10:07:23.21
CBV.dial SSV @10:07:23.81 delay 1 second @10:07:24.10
Allowing 130s for 25.0ml of remaining 1500.0ml @10:07:24.13 Intake.select :open @10:07:24.84 AFV.select :holdingCoil @10:07:24.97 delay 1 second @10:07:26.00 ATV.dial :waste @10:07:26.45
Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:07:26.48 SS.reconfigure SSinhale.with maxSpeed: 122 @10:07:26.59 SS.seek 25ml @10:07:27.08 AS.reconfigure ASnorm @10:07:27.18 AS.home.seek :empty @10:07:30.15
Vacuum too high -- waiting up to 40s @10:07:32.29 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:07:32.33 SS.reconfigure SSinhale.with maxSpeed: 110 @10:07:32.44 SS.seek 25ml @10:07:35.43 Vacuum too high -- waiting up to 40s @10:07:37.53 SS.reconfigure SSinhale.with maxSpeed: 99 @10:07:37.58 SS.seek 25ml @10:07:40.75 Vacuum too high -- waiting up to 40s @10:07:42.84 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:07:42.85 SS.reconfigure SSinhale.with maxSpeed: 89 @10:07:42.90 SS.seek 25ml @10:07:47.09 Vacuum too high -- waiting up to 40s @10:07:49.20 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:07:49.21 SS.reconfigure SSinhale.with maxSpeed: 80 @10:07:49.26 SS.seek 25ml @10:07:52.68 Vacuum too high -- waiting up to 40s @10:07:54.53 SS.reconfigure SSinhale.with maxSpeed: 72 @10:07:54.58 SS.seek 25ml @10:07:58.31 Vacuum too high -- waiting up to 40s @10:08:00.14 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:08:00.15 SS.reconfigure SSinhale.with maxSpeed: 65 @10:08:00.20 SS.seek 25ml @10:08:03.83 Vacuum too high -- waiting up to 40s @10:08:05.66 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:08:05.67 SS.reconfigure SSinhale.with maxSpeed: 59 @10:08:05.72 SS.seek 25ml @10:08:09.79 Stroke completed -- waiting for pressure to equalize ... @10:08:14.25 Intake.select :close @10:08:14.41 SSV.dial :exhaust @10:08:15.22 Exhaust.select :open @10:08:15.34 SS.reconfigure SSexhale @10:08:15.38 SS.seek 0ml @10:08:19.76 ATV.dial :waste @10:08:19.77 ARV.dial between carrier and air1 @10:08:19.86 ASV.dial :waste1 @10:08:24.33
Exhaust.select :close @10:08:24.46 SSV.dial CBV @10:08:24.48
CBV.dial SSV @10:08:25.32 Allowing 130s for 25.0ml of remaining 1475.0ml @10:08:25.33 Intake.select :open @10:08:27.50 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:08:27.52 SS.reconfigure SSinhale.with maxSpeed: 59 @10:08:27.57 SS.seek 25ml @10:08:32.96 Vacuum too high -- waiting up to 40s @10:08:34.38 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:08:34.40 SS.reconfigure SSinhale.with maxSpeed: 53 @10:08:34.44 SS.seek 25ml @10:08:41.09 Vacuum too high -- waiting up to 40s @10:08:42.91 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:08:42.93 SS.reconfigure SSinhale.with maxSpeed: 48 @10:08:42.98 SS.seek 25ml @10:08:50.75 Vacuum too high -- waiting up to 40s @10:08:52.58 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:08:52.60 SS.reconfigure SSinhale.with maxSpeed: 43 @10:08:52.65 SS.seek 25ml @10:09:02.50 Vacuum too high -- waiting up to 40s @10:09:04.56 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:09:04.58 SS.reconfigure SSinhale.with maxSpeed: 39 @10:09:04.63 SS.seek 25ml @10:09:15.23 Stroke completed -- waiting for pressure to equalize ... @10:09:20.92 Intake.select :close @10:09:21.08 SSV.dial :exhaust @10:09:21.88 Exhaust.select :open @10:09:22.00 SS.reconfigure SSexhale @10:09:22.05 SS.seek 0ml @10:09:31.02 Exhaust.select :close @10:09:31.14 SSV.dial CBV @10:09:31.16
CBV.dial SSV @10:09:31.98 Allowing 130s for 25.0ml of remaining 1450.0ml @10:09:31.99 Intake.select :open @10:09:34.16 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:09:34.18 SS.reconfigure SSinhale.with maxSpeed: 39 @10:09:34.23 SS.seek 25ml @10:09:43.89 Vacuum too high -- waiting up to 40s @10:09:46.05 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:09:46.07 SS.reconfigure SSinhale.with maxSpeed: 35 @10:09:46.12 SS.seek 25ml @10:09:57.34 Vacuum too high -- waiting up to 40s @10:09:59.43 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:09:59.44 SS.reconfigure SSinhale.with maxSpeed: 32 @10:09:59.49 SS.seek 25ml @10:10:12.47 Vacuum too high -- waiting up to 40s @10:10:14.29 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:10:14.30 SS.reconfigure SSinhale.with maxSpeed: 29 @10:10:14.35 SS.seek 25ml @10:10:32.08 Vacuum too high -- waiting up to 40s @10:10:34.16 SS.reconfigure SSinhale.with maxSpeed: 26 @10:10:34.21 SS.seek 25ml @10:10:39.33 Stroke completed -- waiting for pressure to equalize ... @10:10:44.39 Intake.select :close @10:10:44.55 SSV.dial :exhaust @10:10:45.35 Exhaust.select :open @10:10:45.47 SS.reconfigure SSexhale @10:10:45.51 SS.seek 0ml @10:10:54.56 Exhaust.select :close @10:10:54.68 SSV.dial CBV @10:10:54.70
CBV.dial SSV @10:10:55.54 Allowing 130s for 25.0ml of remaining 1425.0ml @10:10:55.55 Intake.select :open @10:10:57.72 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:10:57.74 SS.reconfigure SSinhale.with maxSpeed: 26 @10:10:57.79 SS.seek 25ml @10:11:29.69 Vacuum too high -- waiting up to 40s @10:11:32.35 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:11:32.37 SS.reconfigure SSinhale.with maxSpeed: 23 @10:11:32.42 SS.seek 25ml @10:12:12.83 Stroke completed -- waiting for pressure to equalize ... @10:12:19.83 Intake.select :close @10:12:19.99 SSV.dial :exhaust @10:12:20.79 Exhaust.select :open @10:12:20.91 SS.reconfigure SSexhale @10:12:20.95 SS.seek 0ml @10:12:30.06 Exhaust.select :close @10:12:30.18 SSV.dial CBV @10:12:30.20
CBV.dial SSV @10:12:31.02 Allowing 130s for 25.0ml of remaining 1400.0ml @10:12:31.03 Intake.select :open @10:12:33.21 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:12:33.22 SS.reconfigure SSinhale.with maxSpeed: 23 @10:12:33.27 SS.seek 25ml @10:13:49.44 Stroke completed -- waiting for pressure to equalize ... @10:13:57.60 Intake.select :close @10:13:57.75 SSV.dial :exhaust @10:13:58.56 Exhaust.select :open @10:13:58.68 SS.reconfigure SSexhale @10:13:58.73 SS.seek 0ml @10:14:07.46 Exhaust.select :close @10:14:07.58 SSV.dial CBV @10:14:07.60
CBV.dial SSV @10:14:08.43 Allowing 130s for 25.0ml of remaining 1375.0ml @10:14:08.44 Intake.select :open @10:14:10.62 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:14:10.63 SS.reconfigure SSinhale.with maxSpeed: 23 @10:14:10.68 SS.seek 25ml @10:15:27.28 Stroke completed -- waiting for pressure to equalize ... @10:15:36.03 Intake.select :close @10:15:36.19 SSV.dial :exhaust @10:15:36.99 Exhaust.select :open @10:15:37.11 SS.reconfigure SSexhale @10:15:37.15 SS.seek 0ml @10:15:45.90 Exhaust.select :close @10:15:46.01 SSV.dial CBV @10:15:46.05
CBV.dial SSV @10:15:46.87 Allowing 130s for 25.0ml of remaining 1350.0ml @10:15:46.88 Intake.select :open @10:15:49.06 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:15:49.07 SS.reconfigure SSinhale.with maxSpeed: 23 @10:15:49.12 SS.seek 25ml @10:17:05.91 Stroke completed -- waiting for pressure to equalize ... @10:17:14.11 Intake.select :close @10:17:14.27 SSV.dial :exhaust @10:17:15.09 Exhaust.select :open @10:17:15.21 SS.reconfigure SSexhale @10:17:15.26 SS.seek 0ml @10:17:24.24 Exhaust.select :close @10:17:24.36 SSV.dial CBV @10:17:24.41
CBV.dial SSV @10:17:25.20 Allowing 130s for 25.0ml of remaining 1325.0ml @10:17:25.21 Intake.select :open @10:17:27.38 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:17:27.40 SS.reconfigure SSinhale.with maxSpeed: 23 @10:17:27.45 SS.seek 25ml @10:18:43.43 Stroke completed -- waiting for pressure to equalize ... @10:18:51.61 Intake.select :close @10:18:51.76 SSV.dial :exhaust @10:18:52.56 Exhaust.select :open @10:18:52.68 SS.reconfigure SSexhale @10:18:52.73 SS.seek 0ml @10:19:01.90 Exhaust.select :close @10:19:02.02 SSV.dial CBV @10:19:02.04
CBV.dial SSV @10:19:02.85 Allowing 130s for 25.0ml of remaining 1300.0ml @10:19:02.86 Intake.select :open @10:19:05.03 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:19:05.05 SS.reconfigure SSinhale.with maxSpeed: 23 @10:19:05.10 SS.seek 25ml @10:20:21.05 Stroke completed -- waiting for pressure to equalize ... @10:20:29.25 Intake.select :close @10:20:29.41 SSV.dial :exhaust @10:20:30.21 Exhaust.select :open @10:20:30.33 SS.reconfigure SSexhale @10:20:30.38 SS.seek 0ml @10:20:39.75 Exhaust.select :close @10:20:39.87 SSV.dial CBV @10:20:39.89
CBV.dial SSV @10:20:40.73 Allowing 130s for 25.0ml of remaining 1275.0ml @10:20:40.74 Intake.select :open @10:20:42.91 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:20:42.93 SS.reconfigure SSinhale.with maxSpeed: 23 @10:20:42.98 SS.seek 25ml @10:21:59.24 Stroke completed -- waiting for pressure to equalize ... @10:22:07.41 Intake.select :close @10:22:07.57 SSV.dial :exhaust @10:22:08.37 Exhaust.select :open @10:22:08.49 SS.reconfigure SSexhale @10:22:08.54 SS.seek 0ml @10:22:17.50 Exhaust.select :close @10:22:17.62 SSV.dial CBV @10:22:17.64
CBV.dial SSV @10:22:18.48 Allowing 130s for 25.0ml of remaining 1250.0ml @10:22:18.49 Intake.select :open @10:22:20.66 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:22:20.68 SS.reconfigure SSinhale.with maxSpeed: 23 @10:22:20.73 SS.seek 25ml @10:23:13.41 Vacuum too high -- waiting up to 40s @10:23:15.47 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:23:15.49 SS.reconfigure SSinhale.with maxSpeed: 21 @10:23:15.54 SS.seek 25ml @10:23:41.80 Stroke completed -- waiting for pressure to equalize ... @10:23:49.34 Intake.select :close @10:23:49.50 SSV.dial :exhaust @10:23:50.31 Exhaust.select :open @10:23:50.43 SS.reconfigure SSexhale @10:23:50.47 SS.seek 0ml @10:23:59.90 Exhaust.select :close @10:24:00.02 SSV.dial CBV @10:24:00.04
CBV.dial SSV @10:24:00.86 Allowing 130s for 25.0ml of remaining 1225.0ml @10:24:00.87 Intake.select :open @10:24:03.05 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:24:03.06 SS.reconfigure SSinhale.with maxSpeed: 21 @10:24:03.11 SS.seek 25ml @10:25:26.59 Stroke completed -- waiting for pressure to equalize ... @10:25:34.80 Intake.select :close @10:25:34.96 SSV.dial :exhaust @10:25:35.76 Exhaust.select :open @10:25:35.88 SS.reconfigure SSexhale @10:25:35.93 SS.seek 0ml @10:25:45.26 Exhaust.select :close @10:25:45.38 SSV.dial CBV @10:25:45.40
CBV.dial SSV @10:25:46.23 Allowing 130s for 25.0ml of remaining 1200.0ml @10:25:46.24 Intake.select :open @10:25:48.41 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:25:48.43 SS.reconfigure SSinhale.with maxSpeed: 21 @10:25:48.48 SS.seek 25ml @10:27:12.25 Stroke completed -- waiting for pressure to equalize ... @10:27:21.02 Intake.select :close @10:27:21.17 SSV.dial :exhaust @10:27:21.98 Exhaust.select :open @10:27:22.10 SS.reconfigure SSexhale @10:27:22.15 SS.seek 0ml @10:27:31.36 Exhaust.select :close @10:27:31.48 SSV.dial CBV @10:27:31.50
CBV.dial SSV @10:27:32.34 Allowing 130s for 25.0ml of remaining 1175.0ml @10:27:32.35 Intake.select :open @10:27:34.53 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:27:34.54 SS.reconfigure SSinhale.with maxSpeed: 21 @10:27:34.59 SS.seek 25ml @10:28:20.68 Vacuum too high -- waiting up to 40s @10:28:22.86 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:28:22.88 SS.reconfigure SSinhale.with maxSpeed: 19 @10:28:22.92 SS.seek 25ml @10:29:04.83 Stroke completed -- waiting for pressure to equalize ... @10:29:13.64 Intake.select :close @10:29:13.80 SSV.dial :exhaust @10:29:14.61 Exhaust.select :open @10:29:14.73 SS.reconfigure SSexhale @10:29:14.78 SS.seek 0ml @10:29:23.94 Exhaust.select :close @10:29:24.06 SSV.dial CBV @10:29:24.08
CBV.dial SSV @10:29:24.92 Allowing 130s for 25.0ml of remaining 1150.0ml @10:29:24.93 Intake.select :open @10:29:27.10 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:29:27.12 SS.reconfigure SSinhale.with maxSpeed: 19 @10:29:27.17 SS.seek 25ml @10:30:48.66 Vacuum too high -- waiting up to 40s @10:30:51.23 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:30:51.25 SS.reconfigure SSinhale.with maxSpeed: 17 @10:30:51.30 SS.seek 25ml @10:31:03.82 Stroke completed -- waiting for pressure to equalize ... @10:31:12.63 Intake.select :close @10:31:12.78 SSV.dial :exhaust @10:31:14.17 Exhaust.select :open @10:31:14.29 SS.reconfigure SSexhale @10:31:14.34 SS.seek 0ml @10:31:23.70 Exhaust.select :close @10:31:23.82 SSV.dial CBV @10:31:23.84
CBV.dial SSV @10:31:24.66 Allowing 130s for 25.0ml of remaining 1125.0ml @10:31:24.67 Intake.select :open @10:31:26.85 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:31:26.86 SS.reconfigure SSinhale.with maxSpeed: 17 @10:31:26.91 SS.seek 25ml @10:33:09.60 Stroke completed -- waiting for pressure to equalize ... @10:33:20.34 Intake.select :close @10:33:20.49 SSV.dial :exhaust @10:33:21.30 Exhaust.select :open @10:33:21.42 SS.reconfigure SSexhale @10:33:21.46 SS.seek 0ml @10:33:30.53 Exhaust.select :close @10:33:30.65 SSV.dial CBV @10:33:30.67
CBV.dial SSV @10:33:31.50 Allowing 130s for 25.0ml of remaining 1100.0ml @10:33:31.51 Intake.select :open @10:33:33.68 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:33:33.70 SS.reconfigure SSinhale.with maxSpeed: 17 @10:33:33.75 SS.seek 25ml @10:34:27.10 Vacuum too high -- waiting up to 40s @10:34:29.42 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:34:29.43 SS.reconfigure SSinhale.with maxSpeed: 15 @10:34:29.48 SS.seek 25ml @10:35:26.76 Stroke completed -- waiting for pressure to equalize ... @10:35:37.41 Intake.select :close @10:35:37.57 SSV.dial :exhaust @10:35:38.39 Exhaust.select :open @10:35:38.51 SS.reconfigure SSexhale @10:35:38.55 SS.seek 0ml @10:35:47.91 Exhaust.select :close @10:35:48.03 SSV.dial CBV @10:35:48.05
CBV.dial SSV @10:35:48.89 Allowing 130s for 25.0ml of remaining 1075.0ml @10:35:48.90 Intake.select :open @10:35:51.07 Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:35:51.09 SS.reconfigure SSinhale.with maxSpeed: 15 @10:35:51.14 SS.seek 25ml @10:37:48.22 Stroke completed -- waiting for pressure to equalize ... @10:37:58.91 SS.reconfigure SSconfig @10:37:58.96 SS.hold @10:37:59.09 Intake.select :close @10:37:59.25 ShallowSampler::Clogged in MAIN -- progress too slow after 450.0ml @10:37:59.26 SSV.dial :exhaust @10:38:00.06 Exhaust.select :open @10:38:00.18 SS.reconfigure SSexhale @10:38:00.22 SS.seek 0ml @10:38:09.33 Exhaust.select :close @10:38:09.44 Sampled 450.0ml @10:38:09.46 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:38:09.47 Intake.select :close @10:38:09.53 CSV.dial :waste @10:38:09.61 CS.seek 0ml @10:38:09.71 CSV.dial :air @10:38:09.74 SSV.dial CBV,avoiding: :exhaust @10:38:09.79
CBV.dial SSV @10:38:10.68 CS.seek 10ml @10:38:11.78
CTV.dial :air @10:38:12.59 delay 15 seconds @10:38:27.62 SSV.dial :spit,avoiding: :exhaust @10:38:29.01 CTV.dial CSV,avoiding: :intake @10:38:29.09
SS.seek 0ml @10:38:29.16 SSV.dial CBV,avoiding: :exhaust @10:38:34.79 CBV.dial between SSV and waste2 @10:38:35.22 CTV.dial CSV,avoiding: :air @10:38:35.24
CSV.dial CTV @10:38:35.74 CS.reconfigure CSpressurize @10:38:35.78 CS.seek 0ml @10:39:02.17 BubblePoint: Intake @69.7psia, Outlet @14.0psia @10:39:02.18 CBV.dial SSV @10:39:02.54 SS.reconfigure SSconfig @10:39:02.58 SS.seek 10ml @10:39:07.95 delay 15 seconds @10:39:23.09 Evacuated: Intake @36.4psia, Outlet @0.6psia @10:39:23.11 SSV.dial :spit,avoiding: :exhaust @10:39:24.50 SS.reconfigure SSexhale @10:39:24.56 SS.seek 0ml @10:39:29.73 SS.reconfigure SSfast @10:39:29.78 SS.seek 5ml @10:39:32.28 SSV.dial CBV,avoiding: :exhaust @10:39:32.31
CBV.dial SSV @10:39:33.70 CTV.dial :air,avoiding: :intake @10:39:35.10 CSV.dial CTV @10:39:35.14
SS.seek 10ml @10:39:36.14 CSV.dial :air @10:39:36.61 CS.reconfigure CSfast @10:39:36.66 CS.seek 0ml @10:39:37.63
SSV.dial :spit,avoiding: :exhaust @10:39:37.73 CS.coast @10:39:39.00
SS.reconfigure SSexhale @10:39:39.05 SS.seek 0ml @10:39:44.07 SS.reconfigure SSfast @10:39:44.12 SS.seek 5ml @10:39:46.77 SSV.dial CBV,avoiding: :exhaust @10:39:46.80
CBV.dial SSV @10:39:49.20 SSV.dial :spit,avoiding: :exhaust @10:39:50.59 SS.reconfigure SSconfig @10:39:50.64 SS.seek 0ml @10:39:53.80 SS.coast @10:39:53.84 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:39:53.85 Intake.select :close @10:39:53.93 CSV.dial :waste @10:39:54.07 SSV.dial CBV,avoiding: :exhaust @10:39:54.09
CBV.dial SSV @10:39:54.76 CS.seek 0ml @10:39:54.83 CSV.dial :air @10:39:55.47
CTV.dial :air @10:39:55.49 delay 15 seconds @10:39:55.60 CS.seek 10ml @10:40:10.51
SSV.dial :spit,avoiding: :exhaust @10:40:11.90 CTV.dial CSV,avoiding: :intake @10:40:11.94
SS.reconfigure SSexhale @10:40:12.04 SS.seek 0ml @10:40:12.11 SSV.dial CBV,avoiding: :exhaust @10:40:19.33 CBV.dial between SSV and waste2 @10:40:19.76 CSV.dial between air and CTV @10:40:20.09 CS.seek 8.224ml @10:40:25.14 CTV.dial CSV,avoiding: :air @10:40:25.17
CSV.dial CTV @10:40:25.51 CS.reconfigure CSpressurize @10:40:25.55 CS.seek 0ml @10:40:47.14 CSV.dial :air @10:40:47.61 CS.reconfigure CSfast @10:40:47.67 CS.seek 10ml @10:41:11.08 CSV.dial between air and CTV @10:41:11.43 CS.seek 3.048ml @10:41:28.11 CTV.dial CSV,avoiding: :air @10:41:28.14
CSV.dial CTV @10:41:29.15 CS.reconfigure CSpressurize @10:41:29.20 CS.seek 0ml @10:41:36.48 BubblePoint: Intake @69.2psia, Outlet @13.9psia @10:41:36.49 CBV.dial SSV @10:41:36.85 SS.reconfigure SSconfig @10:41:36.90 SS.seek 10ml @10:41:42.31 delay 15 seconds @10:41:57.47 Evacuated: Intake @38.2psia, Outlet @15.8psia @10:41:57.48 SSV.dial :spit,avoiding: :exhaust @10:41:58.87 SS.reconfigure SSexhale @10:41:58.92 SS.seek 0ml @10:42:03.82 SS.reconfigure SSfast @10:42:03.88 SS.seek 5ml @10:42:06.54 SSV.dial CBV,avoiding: :exhaust @10:42:06.56
CBV.dial SSV @10:42:07.95 CTV.dial :air,avoiding: :intake @10:42:09.35 CSV.dial CTV @10:42:09.40
SS.seek 10ml @10:42:10.39 CSV.dial :air @10:42:10.85 CS.reconfigure CSfast @10:42:10.90 CS.seek 0ml @10:42:11.89
SSV.dial :spit,avoiding: :exhaust @10:42:13.02 CS.coast @10:42:13.29
SS.reconfigure SSexhale @10:42:13.33 SS.seek 0ml @10:42:18.25 SS.reconfigure SSfast @10:42:18.30 SS.seek 5ml @10:42:20.99 SSV.dial CBV,avoiding: :exhaust @10:42:21.02
CBV.dial SSV @10:42:23.43 SSV.dial :spit,avoiding: :exhaust @10:42:24.80 SS.reconfigure SSconfig @10:42:24.85 SS.seek 0ml @10:42:28.00 SS.coast @10:42:28.04 Lysing: Adding 1.5ml lysis @10:42:28.05 CBV.dial between CSV and waste1,avoiding: :DBV @10:42:29.63 CTV.dial CSV @10:42:31.02 CSV.dial CSR @10:42:31.84 Pulling 1ml of CSR.lysis @10:42:31.85 CSR.select :lysis @10:42:31.97 CS.reconfigure CSconfig @10:42:32.02 CS.seek 1ml @10:42:35.64 delay 5 seconds @10:42:40.67 CSR.select :air @10:42:40.85 CS.seek 6ml @10:42:54.55 delay 2 seconds @10:42:56.57 CSV.dial :waste1 @10:42:57.91 CS.reconfigure CSfast @10:42:57.96 CS.seek :empty @10:43:12.43 CSV.dial CSR @10:43:13.83 Pulling 1.5ml of CSR.lysis @10:43:13.84 CSR.select :lysis @10:43:13.96 CS.reconfigure CSconfig @10:43:14.01 CS.seek 1.5ml @10:43:19.02 delay 5 seconds @10:43:24.05 CSR.select :air @10:43:24.23 CS.seek 4.5ml @10:43:32.96 delay 2 seconds @10:43:34.98 CSV.dial CTV @10:43:35.47 CS.seek 1.275ml @10:43:44.69 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @10:43:44.71 CH.seek 85C,8:00 @10:46:58.05 CH.hold 5:00,85C @10:51:58.23 Collection Puck Heater at 85C after lysing @10:51:58.25 CS.seek 0ml @10:52:02.72 CTV.dial between spare and air,avoiding: :intake @10:52:03.81 CSV.dial :air @10:52:04.31 CS.seek 5ml @10:52:18.12 CSV.dial CTV @10:52:18.59 delay 2 seconds @10:52:20.61 CSV.dial :air @10:52:21.09 delay 2 seconds @10:52:23.11 CSV.dial CTV @10:52:23.59 delay 2 seconds @10:52:25.61 CSV.dial :air @10:52:26.09 delay 2 seconds @10:52:28.12 CS.seek 0ml @10:52:41.87 Recovering Lysate into Collection syringe @10:52:41.88 CSV.dial CBV @10:52:42.94 CBV.dial CSV @10:52:43.37 CS.seek 5ml @10:52:57.16 delay 5 seconds @10:53:02.19 CTV.dial :air @10:53:02.74 CS.seek 7.001ml @10:53:08.84 delay 5 seconds @10:53:13.87 CSV.dial :air2 @10:53:14.35 CS.seek 10ml @10:53:23.06 CSV.dial between air and CTV,avoiding: CBV @10:53:24.27 Lysing Complete @10:53:24.35 Storing sh1 puck in Collection Clamp to tube 1 @10:53:25.13 SC.reconfigure SCtight @10:53:25.18 SC.seek 1 @10:53:27.31 SC.reconfigure SCloose @10:53:27.54 Elbow.reconfigure TightElbow @10:53:27.59 Elbow.seek 1 @10:53:29.55 Forearm.reconfigure TightForearm @10:53:29.60 Forearm.seek 1 + 700 counts @10:53:30.65 SE.reconfigure SEpick @10:53:30.73 SE.seek beamBreak @10:53:32.25 SE.configure SEconfig @10:53:32.42 Elbow.reconfigure LimpElbow @10:53:32.47 Forearm.reconfigure LooseForearm @10:53:32.51 Forearm.seek 1 @10:53:32.81 Elbow.hold @10:53:32.88 Elbow.reconfigure TightElbow @10:53:32.99 SE.seek 17.11 Pucks below Top Plate @10:53:33.51 Forearm.force -20 @10:53:33.55 Forearm.seek 1 + 349 counts @10:53:33.87 Forearm.reconfigure TightForearm @10:53:33.91 Forearm.seek 1 + 700 counts @10:53:34.21 SE.seek 18.11 Pucks below Top Plate @10:53:34.96 Forearm.reconfigure LooseForearm @10:53:35.01 Forearm.seek :retract @10:53:35.94 Elbow.seek CC @10:53:37.62 Elbow.reconfigure LooseElbow @10:53:37.73 Forearm.seek CC @10:53:38.80 Hand.close @10:53:39.42 CC.seek :open @10:53:43.91 Forearm.seek :retract @10:53:45.02 Elbow.reconfigure TightElbow @10:53:45.07 Elbow.seek 1 @10:53:47.13 Elbow.reconfigure LimpElbow @10:53:47.18 Forearm.seek 1 @10:53:48.17 Elbow.hold @10:53:48.24 Elbow.reconfigure TightElbow @10:53:48.29 Hand.open @10:53:49.06 Forearm.force -20 @10:53:49.10 Forearm.seek 1 + 346 counts @10:53:49.42 Forearm.reconfigure TightForearm @10:53:49.46 Forearm.seek 1 + 700 counts @10:53:49.80 SE.seek :down @10:53:50.97 SE.coast @10:53:51.49
Forearm.reconfigure LooseForearm @10:53:51.66 Forearm.seek :retract @10:53:52.62 SC.coast @10:53:52.66 Tranferring lysate to PRV @10:53:52.67 CSV.dial :air @10:53:53.00 CS.seek 10ml @10:53:53.10 CSV.dial between air and CTV @10:53:53.44 AIV.dial :bypass @10:53:53.53 Beginning samplePassC @10:53:53.55 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:53:53.62 Hand.open @10:53:54.07 Elbow.seek FlushPuck::Garage @10:53:56.08 Elbow.reconfigure LooseElbow @10:53:56.19 Forearm.seek :garage @10:53:57.19 Hand.close @10:53:57.93 Forearm.seek :retract @10:53:58.91 Elbow.reconfigure TightElbow @10:53:58.97 Elbow.seek CC @10:54:01.89 Elbow.reconfigure LooseElbow @10:54:02.00 Forearm.reconfigure TightForearm @10:54:02.05 Forearm.seek CC @10:54:03.37 CC.seek :closed @10:54:07.63 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.96 @10:54:07.70 Hand.open @10:54:08.37 Forearm.reconfigure LooseForearm @10:54:08.42 Forearm.seek :retract @10:54:09.50 AWV.select :air @10:54:09.52 AIV.dial :bypass @10:54:09.53 PSV.dial PSR @10:54:10.94 Pulling 1.5ml of PSR.flush @10:54:10.95 PSR.select :flush @10:54:11.08 PS.seek 1.5ml @10:54:27.17 delay 5 seconds @10:54:32.20 PSV.dial :waste1 @10:54:32.68 PS.seek 0ml @10:54:48.70 PSV.dial PSR @10:54:49.18 PSR.select :air @10:54:49.30 PS.seek 2.5ml @10:55:15.46 PRV.dial CBV @10:55:15.48
CBV.dial PRV @10:55:16.36 PSV.dial :mixloop @10:55:17.40 CTV.dial :air,avoiding: :intake @10:55:17.41 PTV.dial :PRVmixing @10:55:17.50 PS.seek 0ml @10:55:43.61 PSV.dial :air @10:55:44.10 PS.seek 2.5ml @10:56:10.26 PSV.dial :mixloop @10:56:10.72 PS.seek 0ml @10:56:36.89 PSV.dial :air @10:56:37.37 PS.seek 2.5ml @10:57:03.48 PSV.dial :mixloop @10:57:04.54 PS.seek 0ml @10:57:30.73 Garaging FlushPuck in Collection Clamp @10:57:30.81 Elbow.reconfigure TightElbow @10:57:30.86 Elbow.seek CC @10:57:31.10 Elbow.reconfigure LooseElbow @10:57:31.21 Forearm.seek CC @10:57:32.27 Hand.close @10:57:32.93 CC.seek :open @10:57:37.29 Forearm.seek :retract @10:57:38.30 Elbow.reconfigure TightElbow @10:57:38.35 Elbow.seek :garage @10:57:41.57 Elbow.reconfigure LooseElbow @10:57:41.68 Forearm.reconfigure TightForearm @10:57:41.73 Forearm.seek :garage @10:57:42.91 Hand.open @10:57:43.62 Forearm.reconfigure LooseForearm @10:57:43.67 Forearm.seek :retract @10:57:44.69 Connecting PS to CS to transfer sample @10:57:44.70 CBV.dial AIV @10:57:44.71 AIV.dial :bypass @10:57:44.73 PSV.dial :mixloop @10:57:44.74 PTV.dial :PRVmixing @10:57:44.75 PRV.dial AIV @10:57:44.76 CTV.dial :air,avoiding: :intake @10:57:44.78 Loading lyfil puck from tube 2 into Collection Clamp @10:57:45.04 SC.reconfigure SCtight @10:57:45.09 SC.seek 2 @10:57:47.26 SC.reconfigure SCloose @10:57:47.49 Elbow.reconfigure TightElbow @10:57:47.54 Elbow.seek 2 @10:57:49.62 Forearm.reconfigure TightForearm @10:57:49.67 Forearm.seek 2 + 700 counts @10:57:50.72 SE.reconfigure SEpick @10:57:50.80 SE.seek beamBreak @10:57:53.64 SE.configure SEconfig @10:57:53.81 Elbow.seek 2 @10:57:54.01 Forearm.seek 2 + 300 counts @10:57:54.24 Forearm.reconfigure LooseForearm @10:57:54.28 Hand.close @10:57:54.96 SE.seek :down @10:57:55.18
Forearm.seek :retract @10:57:56.15 Elbow.seek CC @10:57:57.59 SE.coast @10:57:57.84
Elbow.reconfigure LooseElbow @10:57:57.94 Forearm.reconfigure TightForearm @10:57:57.99 Forearm.seek CC @10:57:59.32 CC.seek :closed @10:58:03.60 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.00 @10:58:04.16 Hand.open @10:58:04.87 Forearm.reconfigure LooseForearm @10:58:04.92 Forearm.seek :retract @10:58:05.94 SC.coast @10:58:05.98 CTV.dial CSV,avoiding: :intake @10:58:07.37 CSV.dial CTV @10:58:07.81 CS.seek 5ml @10:58:21.54 delay 1:20 @10:59:41.58 CS.seek 2.5ml @10:59:48.95 delay 40 seconds @11:00:28.99 CS.seek 0ml @11:00:36.32 delay 1:00 @11:01:36.35 PSV.dial :air @11:01:36.82 PS.seek 2.5ml @11:02:02.92 PSV.dial :mixloop @11:02:03.38 delay 3 seconds @11:02:06.40 PSV.dial :air @11:02:06.86 delay 2 seconds @11:02:08.88 PSV.dial :mixloop @11:02:09.35 delay 3 seconds @11:02:12.37 PSV.dial :waste1 @11:02:13.71 PS.seek :empty @11:02:39.83 CBV.dial :waste1 @11:02:40.34 delay 5 seconds @11:02:45.37 CBV.dial AIV @11:02:45.87 delay 10 seconds @11:02:55.90 CBV.dial :waste1 @11:02:56.40 delay 10 seconds @11:03:06.43 CBV.dial AIV @11:03:06.93 delay 10 seconds @11:03:16.96 CTV.dial CSV,avoiding: :intake @11:03:16.97 CBV.dial between AIV and waste1 @11:03:17.34 CSV.dial :air @11:03:17.82 CS.seek 10ml @11:03:44.36 CSV.dial CTV @11:03:44.85 CS.seek 0ml @11:04:11.48 CBV.dial :waste1 @11:04:11.81 delay 10 seconds @11:04:21.84 CTV.dial :air,avoiding: :intake @11:04:23.29 Storing lyfil puck in Collection Clamp to tube 1 @11:04:23.47 SC.reconfigure SCtight @11:04:23.52 SC.seek 1 @11:04:25.67 SC.reconfigure SCloose @11:04:25.89 Elbow.reconfigure TightElbow @11:04:25.94 Elbow.seek 1 @11:04:27.96 Forearm.reconfigure TightForearm @11:04:28.01 Forearm.seek 1 + 700 counts @11:04:29.07 SE.reconfigure SEpick @11:04:29.15 SE.seek beamBreak @11:04:30.44 SE.configure SEconfig @11:04:30.62 Elbow.reconfigure LimpElbow @11:04:30.67 Forearm.reconfigure LooseForearm @11:04:30.71 Forearm.seek 1 @11:04:31.00 Elbow.hold @11:04:31.07 Elbow.reconfigure TightElbow @11:04:31.18 SE.seek 18.11 Pucks below Top Plate @11:04:31.70 Forearm.force -20 @11:04:31.75 Forearm.seek 1 + 341 counts @11:04:32.05 Forearm.reconfigure TightForearm @11:04:32.10 Forearm.seek 1 + 700 counts @11:04:32.39 SE.seek 19.11 Pucks below Top Plate @11:04:33.17 Forearm.reconfigure LooseForearm @11:04:33.22 Forearm.seek :retract @11:04:34.71 Elbow.seek CC @11:04:36.39 Elbow.reconfigure LooseElbow @11:04:36.50 Forearm.seek CC @11:04:37.53 Hand.close @11:04:38.21 CC.seek :open @11:04:42.71 Forearm.seek :retract @11:04:43.77 Elbow.reconfigure TightElbow @11:04:43.82 Elbow.seek 1 @11:04:45.84 Elbow.reconfigure LimpElbow @11:04:45.90 Forearm.seek 1 @11:04:46.93 Elbow.hold @11:04:47.00 Elbow.reconfigure TightElbow @11:04:47.05 Hand.open @11:04:47.83 Forearm.force -20 @11:04:47.87 Forearm.seek 1 + 344 counts @11:04:48.19 Forearm.reconfigure TightForearm @11:04:48.24 Forearm.seek 1 + 700 counts @11:04:48.60 SE.seek :down @11:04:49.66 SE.coast @11:04:49.83
Forearm.reconfigure LooseForearm @11:04:49.88 Forearm.seek :retract @11:04:50.80 SC.coast @11:04:50.84 PSV.dial :air @11:04:52.07 PS.seek 0.499ml @11:04:57.93 delay 3 seconds @11:05:00.95 PSV.dial :mixloop @11:05:01.43 PRV.dial :air @11:05:01.93 delay 3 seconds @11:05:04.95 PRV.dial CBV @11:05:05.44 CBV.dial AIV @11:05:05.94 PRV.dial between CBV and air @11:05:06.29 PRV.dial :air @11:05:06.68 PS.reconfigure PSslow @11:05:06.73 PS.seek 0.599ml @11:05:09.30 PRV.dial :waste2 @11:05:10.05 PS.reconfigure PSconfig @11:05:10.10 PS.seek :empty @11:05:16.99 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:05:17.06 Hand.open @11:05:17.45 Elbow.seek FlushPuck::Garage @11:05:19.55 Elbow.reconfigure LooseElbow @11:05:19.66 Forearm.seek :garage @11:05:20.64 Hand.close @11:05:21.45 Forearm.seek :retract @11:05:22.44 Elbow.reconfigure TightElbow @11:05:22.49 Elbow.seek CC @11:05:25.49 Elbow.reconfigure LooseElbow @11:05:25.59 Forearm.reconfigure TightForearm @11:05:25.64 Forearm.seek CC @11:05:27.56 CC.seek :closed @11:05:31.87 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.90 @11:05:31.96 Hand.open @11:05:32.65 Forearm.reconfigure LooseForearm @11:05:32.70 Forearm.seek :retract @11:05:33.81 Clearing PML for modified lysate @11:05:33.82 CTV.dial :air,avoiding: :intake @11:05:33.83 PRV.dial :waste2 @11:05:33.84 PTV.dial :PRV,avoiding: :puck @11:05:34.65 delay 2 seconds @11:05:36.67 PTV.dial :PRVmixing,avoiding: :puck @11:05:37.46 delay 2 seconds @11:05:39.48 PSV.dial :PML @11:05:39.49 delay 2 seconds @11:05:41.52 PRV.dial :air @11:05:42.30 delay 2 seconds @11:05:44.38 PS.seek 1.499ml @11:06:00.40 delay 5 seconds @11:06:05.44 PS.seek 2.5ml @11:06:16.40 PSV.dial :air @11:06:16.89 PS.seek 0ml @11:06:43.02 mixing lysate with speDil @11:06:43.03 PSV.dial :PML @11:06:43.51 CBV.dial :PRV @11:06:43.52 PRV.dial :air @11:06:43.54 delay 2 seconds @11:06:45.56 PRV.dial :speDil @11:06:46.05 delay 2 seconds @11:06:48.14 PS.reconfigure PSslow1 @11:06:48.19 PS.seek 0.225ml @11:07:11.58 delay 5 seconds @11:07:16.61 PRV.dial CBV @11:07:17.40 delay 2 seconds @11:07:19.48 PS.seek 0.475ml @11:07:45.18 delay 5 seconds @11:07:50.21 PRV.dial :air @11:07:50.71 delay 2 seconds @11:07:52.74 PS.seek 0.965ml @11:08:42.65 delay 10 seconds @11:08:52.68 PRV.dial CBV @11:08:53.16 CTV.dial :air,avoiding: :intake @11:08:53.17 PSV.dial :air @11:08:53.63 PS.reconfigure PSconfig @11:08:53.68 PS.seek 2.5ml @11:09:10.15 delay 3 seconds @11:09:13.17 PSV.dial PTV @11:09:13.95 PTV.dial :PRV,avoiding: :puck @11:09:14.74 PS.seek 0ml @11:09:40.90 PSV.dial :air @11:09:41.67 PS.seek 2.5ml @11:10:07.87 delay 3 seconds @11:10:10.89 PSV.dial PTV @11:10:11.65 PTV.dial :PRV,avoiding: :puck @11:10:11.67 PS.seek 0ml @11:10:37.87 Pushing modified lysate into AIV Injection Loop @11:10:37.88 CBV.dial AIV @11:10:37.89 AIV.dial :bypass @11:10:37.90 PRV.dial CBV @11:10:37.92 PSV.dial :air @11:10:38.69 PS.seek 2.5ml @11:11:04.84 delay 3 seconds @11:11:07.86 PSV.dial :PML @11:11:08.34 PTV.dial :PRVmixing,avoiding: :puck @11:11:09.75 PS.reconfigure PSslow1 @11:11:09.82 PS.seek 1.74ml @11:12:27.15 delay 5 seconds @11:12:32.18 AIV.dial :sample @11:12:32.79 AWV.select :waste @11:12:32.96 PS.reconfigure PSslow2 @11:12:33.01 PS.seek 1.22ml @11:14:20.09 delay 15 seconds @11:14:35.12 AIV.dial :bypass @11:14:35.73 PS.reconfigure PSconfig @11:14:35.79 PS.seek 0ml @11:14:49.03 PSV.dial :air @11:14:49.51 PS.seek 2.5ml @11:15:15.62 PSV.dial :PML @11:15:16.10 PS.seek 0ml @11:15:42.19 AIV.dial :coilToMFB @11:15:42.20 AWV.select :air @11:15:42.37 Extracting Nucleic Acid from AIV @11:15:42.40
Beginning samplePassD @11:15:42.46 SPE.hold 2:46:40,55C @11:15:42.52 ASV.dial :SPEdelivery @11:15:42.60
AWV.select :air @11:15:42.62 AIV.dial :bypass @11:15:42.63 PSV.dial PSR @11:15:42.94 delay 20 seconds @11:15:43.79
Pulling 1.5ml of PSR.killMFB @11:15:43.80 PSR.select :killMFB @11:15:43.93 PS.seek 1.5ml @11:15:59.97 delay 5 seconds @11:16:02.97 primeLysateINT @11:16:02.98 AWV.select :air @11:16:02.99 AIV.dial :coilToMFB @11:16:03.01 ATV.dial AIV @11:16:04.75 AS.reconfigure ASslow1 @11:16:04.80 AS.seek 0.299ml @11:16:05.00
PSV.dial :waste1 @11:16:05.51 PS.seek 0ml @11:16:21.62 PSV.dial PSR @11:16:22.23 PSR.select :air @11:16:22.36 PS.seek 2.5ml @11:16:48.50 PRV.dial CBV @11:16:48.53
CBV.dial PRV @11:16:48.54 PSV.dial :mixloop @11:16:49.66 CTV.dial :air,avoiding: :intake @11:16:49.67 PTV.dial :PRVmixing @11:16:49.69 PS.seek 0ml @11:17:05.36 delay 10 seconds @11:17:15.39 ATV.dial :air2 @11:17:15.78
PSV.dial PSR @11:17:16.23 AS.seek 0.329ml @11:17:16.94
Pulling 1.5ml of PSR.flush @11:17:16.95 PSR.select :flush @11:17:17.08 PS.seek 1.5ml @11:17:22.60 ATV.dial :waste2 @11:17:23.16 AS.reconfigure ASnorm @11:17:23.21 AS.seek :empty @11:17:33.06
delay 5 seconds @11:17:38.09 PSV.dial :waste1 @11:17:38.84 PS.seek 0ml @11:17:40.19 Lysate primed! @11:17:40.20 AFV.select :holdingCoil @11:17:40.21 ATV.dial :air2 @11:17:40.84 AS.reconfigure ASslow1 @11:17:40.89 AS.seek 0.069ml @11:17:54.81
PSV.dial PSR @11:17:55.24 delay 3 seconds @11:17:55.30
PSR.select :air @11:17:55.43 PS.seek 2.5ml @11:17:58.26 ATV.dial :AIV @11:17:59.11 AS.seek 0.469ml @11:18:21.50 PRV.dial CBV @11:18:21.53
CBV.dial PRV @11:18:21.54 PSV.dial :mixloop @11:18:22.64 CTV.dial :air,avoiding: :intake @11:18:22.65 PTV.dial :PRVmixing @11:18:22.68 PS.seek 0ml @11:18:48.85 PSV.dial PSR @11:18:50.70 Pulling 1.5ml of PSR.flush @11:18:50.71 PSR.select :flush @11:18:50.85 PS.seek 1.5ml @11:19:06.94 delay 5 seconds @11:19:11.97 PSV.dial :waste1 @11:19:12.48 PS.seek 0ml @11:19:19.80 delay 5 seconds @11:19:24.88 Solid Phase Extraction Heater at 46.5C @11:19:24.89 ATV.dial ASV @11:19:25.29 ASV.dial :SPEdelivery @11:19:25.37 AS.seek 0.041ml @11:19:28.47
PSV.dial PSR @11:19:28.96 PSR.select :air @11:19:29.09 PS.seek 2.5ml @11:19:55.22 PRV.dial CBV @11:19:55.25
CBV.dial PRV @11:19:55.26 PSV.dial :mixloop @11:19:56.33 CTV.dial :air,avoiding: :intake @11:19:56.34 PTV.dial :PRVmixing @11:19:56.36 PS.seek 0ml @11:20:22.50 PSV.dial PSR @11:20:23.63 Pulling 1.5ml of PSR.flush @11:20:23.64 PSR.select :flush @11:20:23.77 PS.seek 1.5ml @11:20:39.79 delay 5 seconds @11:20:44.82 PSV.dial :waste1 @11:20:45.31 PS.seek 0ml @11:20:51.73 delay 10 seconds @11:21:01.35
PSV.dial PSR @11:21:01.75 rinseLysateLineINT @11:21:01.76 AFV.select :carrierSolution @11:21:01.85
PSR.select :air @11:21:01.92 delay 2 seconds @11:21:01.98
PS.seek 2.5ml @11:21:03.94 AS.reconfigure ASconfig @11:21:03.99 AS.seek 1ml @11:21:28.10 PRV.dial CBV @11:21:28.13
CBV.dial PRV @11:21:28.14 PSV.dial :mixloop @11:21:29.22 CTV.dial :air,avoiding: :intake @11:21:29.23 PTV.dial :PRVmixing @11:21:29.25 PS.reconfigure PSslow @11:21:29.31 PS.seek 1.2ml @11:21:56.13 PSV.dial :waste1 @11:21:57.51 PS.reconfigure PSconfig @11:21:57.56 PS.seek 0ml @11:21:59.40 delay 1 second @11:22:00.42 AFV.select :holdingCoil @11:22:00.52 delay 1 second @11:22:01.54 ATV.dial AIV @11:22:02.16 AIV.dial :coilToMFB @11:22:02.17 AWV.select :waste @11:22:02.29 AS.reconfigure ASslow0 @11:22:02.33 AS.seek 0ml @11:22:10.54
PSV.dial :air @11:22:11.62 PS.seek 2.5ml @11:22:37.72 PSV.dial :mixloop @11:22:38.19 PS.reconfigure PSslow @11:22:38.23 PS.seek 0ml @11:23:29.29 PSV.dial :air @11:23:29.77 PS.reconfigure PSconfig @11:23:29.83 PS.seek 2.5ml @11:23:43.23 ATV.dial :air2 @11:23:43.89 AS.reconfigure ASslow1 @11:23:43.94 AS.seek 0.05ml @11:23:54.26 ATV.dial :AIV @11:23:55.05 AS.seek 0.01ml @11:23:55.94
PSV.dial :mixloop @11:23:56.42 PS.reconfigure PSslow @11:23:56.46 PS.seek 0ml @11:24:03.38 ATV.dial :waste @11:24:04.95 AS.seek :empty @11:24:07.40 ethanol wash @11:24:07.41 ATV.dial :air2 @11:24:08.62 AS.reconfigure ASslow2 @11:24:08.68 AS.seek 0.009ml @11:24:18.93 delay 3 seconds @11:24:21.96 Pulling 0.4ml of Ethanol Wash @11:24:21.97 ATV.dial :ethanol @11:24:23.37 AS.reconfigure ASslow1 @11:24:23.42 AS.seek 0.409ml @11:24:47.63
Cleaning Collection Syringe @11:24:47.64 CSV.dial CSR @11:24:48.17 Pulling 4ml of CSR.flush CSR.select :flush @11:24:48.30 CS.seek 4ml @11:24:59.51 delay 5 seconds @11:25:04.54 CSR.select :air @11:25:04.67 CS.seek 10ml @11:25:20.91 CSV.dial CTV @11:25:20.94
CTV.dial CSV @11:25:22.41 CBV.dial :waste1 @11:25:22.92 CS.seek 0ml @11:25:44.12 delay 3 seconds @11:25:47.15 ATV.dial :air1 @11:25:47.54 delay 3 seconds @11:25:49.45
CSV.dial CSR @11:25:50.00 Pulling 4ml of CSR.flush CSR.select :flush @11:25:50.13 CS.seek 4ml @11:25:50.63 AS.seek 0.434ml @11:25:55.90 delay 3 seconds @11:25:58.93 ATV.dial :ethanol @11:25:59.53 AS.seek 0.419ml @11:26:01.33
delay 5 seconds @11:26:02.82 delay 3 seconds @11:26:05.85 ATV.dial ASV @11:26:06.36
CSR.select :air @11:26:06.49 CS.seek 10ml @11:26:07.11 AS.seek 0.092ml @11:26:22.79 CSV.dial CTV @11:26:22.82
CTV.dial CSV @11:26:23.31 CBV.dial :waste1 @11:26:23.33 CS.seek 0ml @11:26:49.95 Garaging FlushPuck in Collection Clamp @11:26:50.02 Elbow.reconfigure TightElbow @11:26:50.07 Elbow.seek CC @11:26:50.34 Elbow.reconfigure LooseElbow @11:26:50.45 Forearm.seek CC @11:26:51.46 Hand.close @11:26:52.10 CC.seek :open @11:26:56.51 Forearm.seek :retract @11:26:57.49 Elbow.reconfigure TightElbow @11:26:57.54 Elbow.seek :garage @11:27:00.70 Elbow.reconfigure LooseElbow @11:27:00.80 Forearm.reconfigure TightForearm @11:27:00.85 Forearm.seek :garage @11:27:01.99 Hand.open @11:27:02.67 Forearm.reconfigure LooseForearm @11:27:02.72 Forearm.seek :retract @11:27:03.75 Whole Cell Archive sampling 1500ml @11:27:03.77 Loading wcr puck from tube 2 into Collection Clamp @11:27:04.03 SC.reconfigure SCtight @11:27:04.08 SC.seek 2 @11:27:06.23 SC.reconfigure SCloose @11:27:06.46 Elbow.reconfigure TightElbow @11:27:06.51 Elbow.seek 2 @11:27:08.64 Forearm.reconfigure TightForearm @11:27:08.69 Forearm.seek 2 + 700 counts @11:27:10.35 SE.reconfigure SEpick @11:27:10.43 SE.seek beamBreak @11:27:13.14 delay 3 seconds @11:27:13.37 SE.configure SEconfig @11:27:13.56 Elbow.seek 2 @11:27:13.77 Forearm.seek 2 + 300 counts @11:27:14.03 Forearm.reconfigure LooseForearm @11:27:14.08 Hand.close @11:27:14.75 SE.seek :down @11:27:14.99 Forearm.seek :retract @11:27:15.92 Elbow.seek CC @11:27:16.16 ATV.dial :waste2 @11:27:16.89 AS.reconfigure ASconfig @11:27:16.94 AS.seek :empty @11:27:17.52 Elbow.reconfigure LooseElbow @11:27:17.64 Forearm.reconfigure TightForearm @11:27:17.69 Forearm.seek CC @11:27:17.76 SE.coast @11:27:19.05 CC.seek :closed @11:27:22.56 ATV.dial :air2 @11:27:22.98 AS.reconfigure ASslow1 @11:27:23.02 AS.seek 1ml @11:27:23.31 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.02 @11:27:23.32 Hand.open @11:27:24.06 Forearm.reconfigure LooseForearm @11:27:24.11 Forearm.seek :retract @11:27:25.12 SC.coast @11:27:25.16 collectSample 1500ml w/bubble point of 55psi @11:27:25.18 Priming 7ml @11:27:25.19 Intake.select :close @11:27:25.20 Exhaust.select :close @11:27:25.21 SS.hold @11:27:25.44 Calibrating pressure sensors @11:27:25.45 CTV.dial :air @11:27:26.85 SSV.dial :spit,avoiding: :exhaust @11:27:26.87 SS.reconfigure SSfast @11:27:26.92 SS.seek 5ml @11:27:29.71 SSV.dial CBV @11:27:29.74 CBV.dial SSV @11:27:31.23 delay 3.8 seconds @11:27:35.18 SSV.dial :spit @11:27:36.57 SS.seek 0ml @11:27:39.22 SSV.dial CBV @11:27:40.63 CTV.dial :intake @11:27:41.43 SSV.dial CBV @11:27:41.45 CBV.dial SSV @11:27:41.47 Allowing 40s for 7.0ml of remaining 7.0ml @11:27:41.48 Intake.select :open @11:27:43.66 Intake @32.0psia, vacuum from 20.0 to 24.0psi @11:27:43.68 SS.reconfigure SSinhale.with maxSpeed: 150 @11:27:43.73 SS.seek 7ml @11:27:50.05 Stroke completed -- waiting for pressure to equalize ... @11:28:02.19 Intake.select :close @11:28:02.35 SSV.dial :exhaust @11:28:03.17 Exhaust.select :open @11:28:03.29 SS.reconfigure SSexhale @11:28:03.34 SS.seek 0ml @11:28:07.03 Exhaust.select :close @11:28:07.14 Sampling 1500.0ml @11:28:07.16 Intake.select :close @11:28:07.17 Exhaust.select :close @11:28:07.18 SS.reconfigure SSconfig @11:28:07.23 SS.hold @11:28:07.45 CTV.dial :intake @11:28:07.47 SSV.dial CBV @11:28:07.49 CBV.dial SSV @11:28:08.31 Allowing 130s for 25.0ml of remaining 1500.0ml @11:28:08.32 Intake.select :open @11:28:10.51 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:28:10.53 SS.reconfigure SSinhale.with maxSpeed: 150 @11:28:10.57 SS.seek 25ml @11:28:14.59 Vacuum too high -- waiting up to 40s @11:28:17.21 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:28:17.23 SS.reconfigure SSinhale.with maxSpeed: 135 @11:28:17.27 SS.seek 25ml @11:28:20.10 Vacuum too high -- waiting up to 40s @11:28:22.43 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:28:22.45 SS.reconfigure SSinhale.with maxSpeed: 122 @11:28:22.50 SS.seek 25ml @11:28:25.52 Vacuum too high -- waiting up to 40s @11:28:28.04 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:28:28.06 SS.reconfigure SSinhale.with maxSpeed: 110 @11:28:28.10 SS.seek 25ml @11:28:31.19 Vacuum too high -- waiting up to 40s @11:28:33.60 SS.reconfigure SSinhale.with maxSpeed: 99 @11:28:33.66 SS.seek 25ml @11:28:36.68 Vacuum too high -- waiting up to 40s @11:28:38.80 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:28:38.82 SS.reconfigure SSinhale.with maxSpeed: 89 @11:28:38.87 SS.seek 25ml @11:28:41.34 Vacuum too high -- waiting up to 40s @11:28:43.44 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:28:43.46 SS.reconfigure SSinhale.with maxSpeed: 80 @11:28:43.50 SS.seek 25ml @11:28:46.30 Vacuum too high -- waiting up to 40s @11:28:48.40 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:28:48.42 SS.reconfigure SSinhale.with maxSpeed: 72 @11:28:48.47 SS.seek 25ml @11:28:51.43 Vacuum too high -- waiting up to 40s @11:28:53.42 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:28:53.44 SS.reconfigure SSinhale.with maxSpeed: 65 @11:28:53.49 SS.seek 25ml @11:28:55.60 Stroke completed -- waiting for pressure to equalize ... @11:28:59.49 Intake.select :close @11:28:59.65 SSV.dial :exhaust @11:29:00.47 Exhaust.select :open @11:29:00.59 SS.reconfigure SSexhale @11:29:00.64 SS.seek 0ml @11:29:09.64 Exhaust.select :close @11:29:09.76 SSV.dial CBV @11:29:09.78 CBV.dial SSV @11:29:10.61 Allowing 130s for 25.0ml of remaining 1475.0ml @11:29:10.62 Intake.select :open @11:29:12.81 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:29:12.83 SS.reconfigure SSinhale.with maxSpeed: 65 @11:29:12.88 SS.seek 25ml @11:29:18.87 Vacuum too high -- waiting up to 40s @11:29:20.73 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:29:20.75 SS.reconfigure SSinhale.with maxSpeed: 59 @11:29:20.80 SS.seek 25ml @11:29:26.01 Vacuum too high -- waiting up to 40s @11:29:27.85 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:29:27.87 SS.reconfigure SSinhale.with maxSpeed: 53 @11:29:27.92 SS.seek 25ml @11:29:34.18 Vacuum too high -- waiting up to 40s @11:29:36.02 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:29:36.04 SS.reconfigure SSinhale.with maxSpeed: 48 @11:29:36.09 SS.seek 25ml @11:29:44.62 Vacuum too high -- waiting up to 40s @11:29:46.45 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:29:46.47 SS.reconfigure SSinhale.with maxSpeed: 43 @11:29:46.52 SS.seek 25ml @11:29:58.82 Stroke completed -- waiting for pressure to equalize ... @11:30:03.98 Intake.select :close @11:30:04.14 SSV.dial :exhaust @11:30:04.97 Exhaust.select :open @11:30:05.09 SS.reconfigure SSexhale @11:30:05.14 SS.seek 0ml @11:30:14.02 Exhaust.select :close @11:30:14.14 SSV.dial CBV @11:30:14.16 CBV.dial SSV @11:30:15.00 Allowing 130s for 25.0ml of remaining 1450.0ml @11:30:15.01 Intake.select :open @11:30:17.20 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:30:17.22 SS.reconfigure SSinhale.with maxSpeed: 43 @11:30:17.27 SS.seek 25ml @11:30:36.43 Vacuum too high -- waiting up to 40s @11:30:38.04 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:30:38.06 SS.reconfigure SSinhale.with maxSpeed: 39 @11:30:38.11 SS.seek 25ml @11:30:44.59 delay 5 seconds @11:30:49.62 ATV.dial ASV @11:30:50.58 ASV.dial :SPEdelivery @11:30:50.60 AS.seek 0.02ml @11:31:03.13 Stroke completed -- waiting for pressure to equalize ... @11:31:08.93 Intake.select :close @11:31:09.09 SSV.dial :exhaust @11:31:09.91 Exhaust.select :open @11:31:10.03 SS.reconfigure SSexhale @11:31:10.08 SS.seek 0ml @11:31:19.25 Exhaust.select :close @11:31:19.37 SSV.dial CBV @11:31:19.39 CBV.dial SSV @11:31:20.21 Allowing 130s for 25.0ml of remaining 1425.0ml @11:31:20.22 Intake.select :open @11:31:22.41 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:31:22.43 SS.reconfigure SSinhale.with maxSpeed: 39 @11:31:22.48 SS.seek 25ml @11:31:59.31 Vacuum too high -- waiting up to 40s @11:32:01.14 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:32:01.16 SS.reconfigure SSinhale.with maxSpeed: 35 @11:32:01.83 SS.seek 25ml @11:32:11.05 Stroke completed -- waiting for pressure to equalize ... @11:32:14.95 Intake.select :close @11:32:15.11 SSV.dial :exhaust @11:32:15.94 Exhaust.select :open @11:32:16.06 SS.reconfigure SSexhale @11:32:16.12 SS.seek 0ml @11:32:24.94 Exhaust.select :close @11:32:25.05 SSV.dial CBV @11:32:25.09 CBV.dial SSV @11:32:25.91 Allowing 130s for 25.0ml of remaining 1400.0ml @11:32:25.92 Intake.select :open @11:32:28.11 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:32:28.13 SS.reconfigure SSinhale.with maxSpeed: 35 @11:32:28.18 SS.seek 25ml @11:33:18.78 Stroke completed -- waiting for pressure to equalize ... @11:33:23.93 Intake.select :close @11:33:24.09 SSV.dial :exhaust @11:33:24.91 Exhaust.select :open @11:33:25.03 SS.reconfigure SSexhale @11:33:25.08 SS.seek 0ml @11:33:34.13 Exhaust.select :close @11:33:34.25 SSV.dial CBV @11:33:34.27 CBV.dial SSV @11:33:35.11 Allowing 130s for 25.0ml of remaining 1375.0ml @11:33:35.12 Intake.select :open @11:33:37.31 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:33:37.33 SS.reconfigure SSinhale.with maxSpeed: 35 @11:33:37.38 SS.seek 25ml @11:34:07.98 delay 5 seconds @11:34:13.01 ATV.dial :air2 @11:34:14.04 AS.seek 0.52ml @11:34:27.61 Stroke completed -- waiting for pressure to equalize ... @11:34:33.36 Intake.select :close @11:34:33.52 SSV.dial :exhaust @11:34:34.35 Exhaust.select :open @11:34:34.47 SS.reconfigure SSexhale @11:34:34.52 SS.seek 0ml @11:34:43.64 Exhaust.select :close @11:34:43.76 SSV.dial CBV @11:34:43.78 CBV.dial SSV @11:34:44.60 Allowing 130s for 25.0ml of remaining 1350.0ml @11:34:44.61 Intake.select :open @11:34:46.80 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:34:46.82 SS.reconfigure SSinhale.with maxSpeed: 35 @11:34:46.87 SS.seek 25ml @11:35:37.27 Stroke completed -- waiting for pressure to equalize ... @11:35:43.66 Intake.select :close @11:35:43.82 SSV.dial :exhaust @11:35:44.65 Exhaust.select :open @11:35:44.77 SS.reconfigure SSexhale @11:35:44.82 SS.seek 0ml @11:35:53.74 Exhaust.select :close @11:35:53.86 SSV.dial CBV @11:35:53.88 CBV.dial SSV @11:35:54.72 Allowing 130s for 25.0ml of remaining 1325.0ml @11:35:54.73 Intake.select :open @11:35:54.81 delay 5 seconds @11:35:56.91 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:35:56.93 SS.reconfigure SSinhale.with maxSpeed: 35 @11:35:56.98 SS.seek 25ml @11:35:59.84 ATV.dial ASV @11:36:00.78 ASV.dial :wasteR @11:36:01.15 AS.seek 0.3ml @11:36:40.15 Vacuum too high -- waiting up to 40s @11:36:42.25 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:36:42.27 SS.reconfigure SSinhale.with maxSpeed: 32 @11:36:42.32 SS.seek 25ml @11:36:45.66 delay 20 seconds @11:36:50.75 Stroke completed -- waiting for pressure to equalize ... @11:36:55.89 Intake.select :close @11:36:56.05 SSV.dial :exhaust @11:36:56.88 Exhaust.select :open @11:36:57.00 SS.reconfigure SSexhale @11:36:57.05 SS.seek 0ml @11:37:05.70 AS.seek 0.07ml @11:37:06.19 Exhaust.select :close @11:37:06.31 SSV.dial CBV @11:37:06.33 CBV.dial SSV @11:37:07.16 Allowing 130s for 25.0ml of remaining 1300.0ml @11:37:07.17 Intake.select :open @11:37:09.36 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:37:09.38 SS.reconfigure SSinhale.with maxSpeed: 32 @11:37:09.43 SS.seek 25ml @11:37:52.19 delay 20 seconds @11:38:04.22 Stroke completed -- waiting for pressure to equalize ... @11:38:10.60 Intake.select :close @11:38:10.77 SSV.dial :exhaust @11:38:11.60 Exhaust.select :open @11:38:11.72 SS.reconfigure SSexhale @11:38:11.77 SS.seek 0ml @11:38:12.21 ATV.dial :waste2 @11:38:12.94 AS.reconfigure ASconfig @11:38:12.99 AS.seek :empty @11:38:17.36 Elution @11:38:17.37 ATV.dial :air1 @11:38:18.45 AS.reconfigure ASslow1 @11:38:18.50 AS.seek 0.049ml @11:38:20.96 Exhaust.select :close @11:38:21.07 SSV.dial CBV @11:38:21.10 CBV.dial SSV @11:38:21.93 Allowing 130s for 25.0ml of remaining 1275.0ml @11:38:21.94 Intake.select :open @11:38:24.12 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:38:24.14 SS.reconfigure SSinhale.with maxSpeed: 32 @11:38:24.19 SS.seek 25ml @11:38:28.78 delay 3 seconds @11:38:31.81 ATV.dial :ElutionWater @11:38:32.48 AS.seek 0.109ml @11:38:44.86 delay 3 seconds @11:38:47.89 ATV.dial ASV,via: :air2 @11:38:49.87 ASV.dial :SPEelution @11:38:50.44 AFV.select :carrierSolution @11:38:50.60 AS.reconfigure ASnorm @11:38:50.66 AS.seek 0.759ml @11:39:14.43 Vacuum too high -- waiting up to 40s @11:39:16.56 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:39:16.58 SS.reconfigure SSinhale.with maxSpeed: 29 @11:39:16.63 SS.seek 25ml @11:39:22.04 Stroke completed -- waiting for pressure to equalize ... @11:39:23.75 delay 3 seconds @11:39:26.77 AFV.select :holdingCoil @11:39:26.92 AS.reconfigure ASslow1 @11:39:26.97 AS.seek 0.474ml @11:39:27.21 Intake.select :close @11:39:27.37 SSV.dial :exhaust @11:39:28.19 Exhaust.select :open @11:39:28.31 SS.reconfigure SSexhale @11:39:28.36 SS.seek 0ml @11:39:37.49 Exhaust.select :close @11:39:37.61 SSV.dial CBV @11:39:37.63 CBV.dial SSV @11:39:38.47 Allowing 130s for 25.0ml of remaining 1250.0ml @11:39:38.48 Intake.select :open @11:39:40.67 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:39:40.68 SS.reconfigure SSinhale.with maxSpeed: 29 @11:39:40.74 SS.seek 25ml @11:40:24.58 delay 5 seconds @11:40:29.68 AS.reconfigure ASslow2 @11:40:29.73 AS.seek 0.439ml @11:40:41.04 Stroke completed -- waiting for pressure to equalize ... @11:40:47.46 Intake.select :close @11:40:47.62 SSV.dial :exhaust @11:40:48.43 Exhaust.select :open @11:40:48.55 SS.reconfigure SSexhale @11:40:48.60 SS.seek 0ml @11:40:57.51 Exhaust.select :close @11:40:57.63 SSV.dial CBV @11:40:57.65 CBV.dial SSV @11:40:58.47 Allowing 130s for 25.0ml of remaining 1225.0ml @11:40:58.48 Intake.select :open @11:41:00.67 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:41:00.69 SS.reconfigure SSinhale.with maxSpeed: 29 @11:41:00.74 SS.seek 25ml @11:41:05.05 delay 1:00 @11:41:57.06 Vacuum too high -- waiting up to 40s @11:41:59.42 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:41:59.44 SS.reconfigure SSinhale.with maxSpeed: 26 @11:41:59.49 SS.seek 25ml @11:42:04.98 Stroke completed -- waiting for pressure to equalize ... @11:42:05.07 pushing eluate to holding coil @11:42:05.14 AS.seek 0.279ml @11:42:10.76 Intake.select :close @11:42:10.92 SSV.dial :exhaust @11:42:11.74 Exhaust.select :open @11:42:11.86 SS.reconfigure SSexhale @11:42:11.91 SS.seek 0ml @11:42:21.08 Exhaust.select :close @11:42:21.20 SSV.dial CBV @11:42:21.22 CBV.dial SSV @11:42:22.05 Allowing 130s for 25.0ml of remaining 1200.0ml @11:42:22.06 Intake.select :open @11:42:24.25 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:42:24.27 SS.reconfigure SSinhale.with maxSpeed: 26 @11:42:24.32 SS.seek 25ml @11:43:31.98 Stroke completed -- waiting for pressure to equalize ... @11:43:39.62 Intake.select :close @11:43:39.78 SSV.dial :exhaust @11:43:40.58 Exhaust.select :open @11:43:40.70 SS.reconfigure SSexhale @11:43:40.75 SS.seek 0ml @11:43:49.87 Exhaust.select :close @11:43:49.99 SSV.dial CBV @11:43:50.01 CBV.dial SSV @11:43:50.85 Allowing 130s for 25.0ml of remaining 1175.0ml @11:43:50.86 Intake.select :open @11:43:53.05 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:43:53.07 SS.reconfigure SSinhale.with maxSpeed: 26 @11:43:53.11 SS.seek 25ml @11:44:46.18 delay 30 seconds @11:44:47.15 Vacuum too high -- waiting up to 40s @11:44:50.01 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:44:50.03 SS.reconfigure SSinhale.with maxSpeed: 23 @11:44:50.08 SS.seek 25ml @11:45:05.43 Stroke completed -- waiting for pressure to equalize ... @11:45:13.06 Intake.select :close @11:45:13.22 SSV.dial :exhaust @11:45:14.04 Exhaust.select :open @11:45:14.16 SS.reconfigure SSexhale @11:45:14.21 SS.seek 0ml @11:45:16.21 ATV.dial :waste,via: :air3 @11:45:17.82 AS.reconfigure ASconfig @11:45:17.87 AS.seek :empty @11:45:23.13 Exhaust.select :close @11:45:23.25 SSV.dial CBV @11:45:23.27 CBV.dial SSV @11:45:24.12 Allowing 130s for 25.0ml of remaining 1150.0ml @11:45:24.13 Intake.select :open @11:45:26.32 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:45:26.34 SS.reconfigure SSinhale.with maxSpeed: 23 @11:45:26.39 SS.seek 25ml @11:45:34.27 Parking Bleach in LysateSource line and SPEcolumn @11:45:34.28 ATV.dial :air3 @11:45:35.09 AS.reconfigure ASslow1 @11:45:35.14 AS.seek 0.029ml @11:45:41.51 ATV.dial :ElutionWater @11:45:42.11 AS.seek 0.059ml @11:45:48.44 ATV.dial :air3 @11:45:49.05 AS.seek 0.069ml @11:45:51.37 delay 2 seconds @11:45:53.39 ATV.dial :bleach @11:45:53.87 AS.seek 0.769ml @11:46:43.10 Stroke completed -- waiting for pressure to equalize ... @11:46:53.33 Intake.select :close @11:46:53.49 SSV.dial :exhaust @11:46:54.32 Exhaust.select :open @11:46:54.44 SS.reconfigure SSexhale @11:46:54.49 SS.seek 0ml @11:47:03.40 Exhaust.select :close @11:47:03.52 SSV.dial CBV @11:47:03.54 CBV.dial SSV @11:47:04.37 Allowing 130s for 25.0ml of remaining 1125.0ml @11:47:04.38 Intake.select :open @11:47:06.56 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:47:06.58 SS.reconfigure SSinhale.with maxSpeed: 23 @11:47:06.63 SS.seek 25ml @11:47:50.78 Vacuum too high -- waiting up to 40s @11:47:53.66 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:47:53.68 SS.reconfigure SSinhale.with maxSpeed: 21 @11:47:53.73 SS.seek 25ml @11:48:14.90 delay 3 seconds @11:48:17.93 ATV.dial :air3 @11:48:18.46 delay 3 seconds @11:48:21.55 AS.seek 0.794ml @11:48:26.85 delay 3 seconds @11:48:29.88 ATV.dial :bleach @11:48:29.98 Stroke completed -- waiting for pressure to equalize ... @11:48:30.49 AS.seek 0.779ml @11:48:33.80 delay 3 seconds @11:48:36.82 ATV.dial :AIV @11:48:38.02 AS.reconfigure ASconfig @11:48:38.07 AS.seek 0.015ml @11:48:40.94 Intake.select :close @11:48:41.10 SSV.dial :exhaust @11:48:41.94 Exhaust.select :open @11:48:42.06 SS.reconfigure SSexhale @11:48:42.11 SS.seek 0ml @11:48:51.55 Exhaust.select :close @11:48:51.66 SSV.dial CBV @11:48:51.69 CBV.dial SSV @11:48:52.51 Allowing 130s for 25.0ml of remaining 1100.0ml @11:48:52.52 Intake.select :open @11:48:54.71 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:48:54.73 SS.reconfigure SSinhale.with maxSpeed: 21 @11:48:54.78 SS.seek 25ml @11:49:22.31 ATV.dial :waste @11:49:23.73 AS.seek :empty @11:49:24.90 ATV.dial :air3 @11:49:25.55 AS.reconfigure ASslow1 @11:49:25.60 AS.seek 0.009ml @11:49:27.95 delay 2 seconds @11:49:29.97 ATV.dial :bleach @11:49:30.44 AS.seek 0.579ml @11:49:40.44 Vacuum too high -- waiting up to 40s @11:49:43.56 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:49:43.58 SS.reconfigure SSinhale.with maxSpeed: 19 @11:49:43.63 SS.seek 25ml @11:50:25.62 Stroke completed -- waiting for pressure to equalize ... @11:50:37.71 Intake.select :close @11:50:37.87 SSV.dial :exhaust @11:50:38.70 Exhaust.select :open @11:50:38.82 SS.reconfigure SSexhale @11:50:38.87 SS.seek 0ml @11:50:47.85 Exhaust.select :close @11:50:47.97 SSV.dial CBV @11:50:47.99 CBV.dial SSV @11:50:48.82 Allowing 130s for 25.0ml of remaining 1075.0ml @11:50:48.83 Intake.select :open @11:50:51.01 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:50:51.03 SS.reconfigure SSinhale.with maxSpeed: 19 @11:50:51.08 SS.seek 25ml @11:51:25.32 delay 3 seconds @11:51:28.35 ATV.dial :air3 @11:51:28.89 delay 3 seconds @11:51:31.98 AS.seek 0.604ml @11:51:37.30 delay 3 seconds @11:51:40.09 Vacuum too high -- waiting up to 40s @11:51:40.37 ATV.dial :bleach @11:51:40.99 AS.seek 0.589ml @11:51:43.80 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:51:43.82 SS.reconfigure SSinhale.with maxSpeed: 17 @11:51:43.86 SS.seek 25ml @11:51:44.28 delay 3 seconds @11:51:47.30 ATV.dial :ASV @11:51:48.29 ASV.dial :wasteR @11:51:48.86 AS.seek 0ml @11:52:33.03 Stroke completed -- waiting for pressure to equalize ... @11:52:47.02 Intake.select :close @11:52:47.18 SSV.dial :exhaust @11:52:47.99 Exhaust.select :open @11:52:48.11 SS.reconfigure SSexhale @11:52:48.17 SS.seek 0ml @11:52:57.40 Exhaust.select :close @11:52:57.52 SSV.dial CBV @11:52:57.54 CBV.dial SSV @11:52:58.37 Allowing 130s for 25.0ml of remaining 1050.0ml @11:52:58.38 Intake.select :open @11:53:00.57 Intake @32.0psia, vacuum from 20.0 to 24.0psi @11:53:00.59 SS.reconfigure SSinhale.with maxSpeed: 17 @11:53:00.63 SS.seek 25ml @11:53:47.62 delay 1:00 @11:53:52.16 Vacuum too high -- waiting up to 40s @11:53:56.09 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:53:56.11 SS.reconfigure SSinhale.with maxSpeed: 15 @11:53:56.16 SS.seek 25ml @11:54:47.65 AFV.select :carrierSolution @11:54:47.75 delay 2 seconds @11:54:49.78 AS.reconfigure ASconfig @11:54:49.83 AS.seek 1ml @11:54:55.17 Stroke completed -- waiting for pressure to equalize ... @11:55:08.47 SS.reconfigure SSconfig @11:55:08.53 SS.hold @11:55:08.66 Intake.select :close @11:55:08.82 ShallowSampler::Clogged in WCR -- progress too slow after 475.0ml @11:55:08.84 SSV.dial :exhaust @11:55:09.65 Exhaust.select :open @11:55:10.43 SS.reconfigure SSexhale @11:55:10.48 SS.seek 0ml @11:55:19.87 Exhaust.select :close @11:55:19.98 Sampled 475.0ml @11:55:20.00 Drying puck before fixing for 4:00 @11:55:20.01 CBV.dial :waste2b @11:55:20.84 delay 1:00 @11:55:47.62 delay 1 second @11:55:48.64 AFV.select :holdingCoil @11:55:48.74 delay 1 second @11:55:49.77 ATV.dial ASV @11:55:49.78 ASV.dial :wasteR @11:55:49.80 AS.reconfigure ASslow1 @11:55:49.85 AS.seek 0ml @11:56:20.87 CSV.dial :air @11:56:21.37 CS.reconfigure CSfast @11:56:21.42 CS.seek 10ml @11:56:45.48 Pumping wcr puck from tube 2 up to 55psi... @11:56:45.50 CBV.dial SSV @11:56:46.88 CBV.dial between CSV and waste2b @11:56:47.91 CSV.dial between air and CTV @11:56:48.27 CS.seek 7.175ml @11:56:55.87 CTV.dial CSV,avoiding: :air @11:56:55.89 CSV.dial CTV @11:56:56.76 CS.reconfigure CSpressurize @11:56:56.81 CS.seek 0ml @11:57:14.69 CBV.dial :waste2b @11:57:15.05 CS.reconfigure CSconfig @11:57:15.10 CS.seek :empty @11:57:17.08 delay 1:00 @11:58:17.11 CTV.dial :air,avoiding: :intake @11:58:18.53 Fix #1 of 2 (with rnal) for 2:00 @11:58:18.54 CSV.dial CSR @11:58:19.06 Pulling 2ml of CSR.rnal @11:58:19.08 CSR.select :rnal @11:58:19.20 CS.seek 2ml @11:58:25.33 delay 5 seconds @11:58:30.36 CSR.select :air @11:58:30.49 CS.reconfigure CSfast @11:58:30.54 CS.seek 10ml @11:58:49.86 Pumping wcr puck from tube 2 up to 55psi... @11:58:49.88 CBV.dial SSV @11:58:51.24 CBV.dial between CSV and waste2b @11:58:52.27 CSV.dial between air and CTV @11:58:52.92 CS.seek 7.149ml @11:59:00.53 CTV.dial CSV,avoiding: :air @11:59:00.55 CSV.dial CTV @11:59:02.04 CS.reconfigure CSpressurize @11:59:02.09 CS.seek 0ml @11:59:11.25 delay 20 seconds @11:59:20.32 delay 2:00 @11:59:31.27 ATV.dial :air3 @11:59:32.71 AS.seek 0.59ml @12:01:20.35 CBV.dial :waste2b @12:01:20.72 CS.reconfigure CSconfig @12:01:20.77 CS.seek :empty @12:01:22.24 delay 1:00 @12:01:31.69 delay 3 seconds @12:01:34.72 ATV.dial :ASV @12:01:36.06 ASV.dial :wasteR @12:01:36.14 AS.seek 0.09ml @12:02:22.27 CTV.dial :air,avoiding: :intake @12:02:24.29 Fix #2 of 2 (with rnal) for 2:00 @12:02:24.30 CSV.dial CSR @12:02:24.84 Pulling 2ml of CSR.rnal @12:02:24.85 CSR.select :rnal @12:02:24.97 CS.seek 2ml @12:02:31.18 delay 5 seconds @12:02:36.21 CSR.select :air @12:02:36.34 CS.reconfigure CSfast @12:02:36.39 CS.seek 10ml @12:02:55.49 Pumping wcr puck from tube 2 up to 55psi... @12:02:55.51 CBV.dial SSV @12:02:56.89 CBV.dial between CSV and waste2b @12:02:57.93 CSV.dial between air and CTV @12:02:58.58 CS.seek 7.098ml @12:03:06.25 CTV.dial CSV,avoiding: :air @12:03:06.27 CSV.dial CTV @12:03:07.76 CS.reconfigure CSpressurize @12:03:07.81 CS.seek 0ml @12:03:16.99 delay 30 seconds @12:03:24.82 delay 2:00 @12:03:47.01 ATV.dial :extSrc @12:03:47.75 delay 3 seconds @12:03:50.78 ATV.dial :ElutionWater @12:03:51.84 AS.seek 0.12ml @12:03:58.16 ATV.dial :air3 @12:03:58.76 AS.seek 0.13ml @12:04:01.05 delay 2 seconds @12:04:03.07 ATV.dial :bleach @12:04:03.54 AS.seek 0.23ml @12:04:23.91 delay 3 seconds @12:04:26.94 ATV.dial :air3 @12:04:27.47 delay 3 seconds @12:04:30.56 AS.seek 0.255ml @12:04:35.83 delay 3 seconds @12:04:38.86 ATV.dial :bleach @12:04:39.46 AS.seek 0.24ml @12:04:42.74 delay 3 seconds @12:04:45.77 ATV.dial :ASV @12:04:46.75 ASV.dial :SPEdelivery @12:04:47.11 AFV.select :carrierSolution @12:04:47.22 delay 2 seconds @12:04:49.24 AS.reconfigure ASconfig @12:04:49.29 AS.seek 0.5ml @12:05:04.57 delay 1 second @12:05:05.59 AFV.select :holdingCoil @12:05:05.74 AS.reconfigure ASslow1 @12:05:05.79 AS.seek 0.17ml @12:05:24.85 CBV.dial :waste2b @12:05:25.21 CS.reconfigure CSconfig @12:05:25.26 CS.seek :empty @12:05:27.90 delay 1:00 @12:06:12.44 SPE.hold 2:46:40,95C @12:06:12.49 delay 3:00 @12:06:27.93 CTV.dial :air,avoiding: :intake @12:06:29.47 Storing wcr puck in Collection Clamp to tube 1 @12:06:29.66 SC.reconfigure SCtight @12:06:29.71 SC.seek 1 @12:06:32.00 SC.reconfigure SCloose @12:06:32.23 Elbow.reconfigure TightElbow @12:06:32.28 Elbow.seek 1 @12:06:34.20 Forearm.reconfigure TightForearm @12:06:34.25 Forearm.seek 1 + 700 counts @12:06:35.40 SE.reconfigure SEpick @12:06:36.10 SE.seek beamBreak @12:06:37.36 SE.configure SEconfig @12:06:37.54 Elbow.reconfigure LimpElbow @12:06:37.60 Forearm.reconfigure LooseForearm @12:06:37.65 Forearm.seek 1 @12:06:37.95 Elbow.hold @12:06:38.03 Elbow.reconfigure TightElbow @12:06:38.15 SE.seek 19.11 Pucks below Top Plate @12:06:38.75 Forearm.force -20 @12:06:38.79 Forearm.seek 1 + 343 counts @12:06:39.12 Forearm.reconfigure TightForearm @12:06:39.17 Forearm.seek 1 + 700 counts @12:06:39.45 SE.seek 20.11 Pucks below Top Plate @12:06:40.17 Forearm.reconfigure LooseForearm @12:06:40.23 Forearm.seek :retract @12:06:41.16 Elbow.seek CC @12:06:42.82 Elbow.reconfigure LooseElbow @12:06:42.93 Forearm.seek CC @12:06:44.00 Hand.close @12:06:44.80 CC.seek :open @12:06:49.36 Forearm.seek :retract @12:06:50.42 Elbow.reconfigure TightElbow @12:06:50.47 Elbow.seek 1 @12:06:52.47 Elbow.reconfigure LimpElbow @12:06:52.53 Forearm.seek 1 @12:06:53.56 Elbow.hold @12:06:53.64 Elbow.reconfigure TightElbow @12:06:53.69 Hand.open @12:06:54.43 Forearm.force -20 @12:06:54.48 Forearm.seek 1 + 346 counts @12:06:54.81 Forearm.reconfigure TightForearm @12:06:54.86 Forearm.seek 1 + 700 counts @12:06:55.26 SE.seek :down @12:06:56.17 SE.coast @12:06:56.42 Forearm.reconfigure LooseForearm @12:06:56.47 Forearm.seek :retract @12:06:57.44 SC.coast @12:06:57.48 Starting finalPurge @12:06:57.50 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:06:57.58 Hand.open @12:06:57.94 Elbow.seek FlushPuck::Garage @12:07:00.63 Elbow.reconfigure LooseElbow @12:07:00.74 Forearm.seek :garage @12:07:01.77 Hand.close @12:07:02.56 Forearm.seek :retract @12:07:03.58 Elbow.reconfigure TightElbow @12:07:03.63 Elbow.seek CC @12:07:06.57 Elbow.reconfigure LooseElbow @12:07:06.67 Forearm.reconfigure TightForearm @12:07:06.72 Forearm.seek CC @12:07:08.09 CC.seek :closed @12:07:12.35 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.22 @12:07:12.42 Hand.open @12:07:13.12 Forearm.reconfigure LooseForearm @12:07:13.17 Forearm.seek :retract @12:07:14.21 Flushing the Collection Valve::Manifold @12:07:14.35 CSV.dial CSR @12:07:14.91 Pulling 5ml of CSR.flush CSR.select :flush @12:07:15.04 CS.seek 5ml @12:07:28.81 delay 5 seconds @12:07:33.84 CSR.select :air @12:07:34.02 CS.reconfigure CSfast @12:07:34.07 CS.seek 7.501ml @12:07:41.09 CSV.dial :waste @12:07:42.50 CS.reconfigure CSconfig @12:07:42.55 CS.seek 0ml @12:08:02.68 Flushing lower manifold's waste1 port @12:08:02.82 CSV.dial :flush @12:08:03.32 CS.reconfigure CSfast @12:08:03.36 CS.seek 5ml @12:08:16.18 delay 5 seconds @12:08:21.21 CSV.dial :air @12:08:21.77 CS.seek 7ml @12:08:27.65 CSV.dial CTV @12:08:27.68
CTV.dial CSV @12:08:29.21 CBV.dial :waste1 @12:08:30.05 CS.seek 0ml @12:08:47.62 Flushing lower manifold's waste2b port @12:08:47.76 CSV.dial :flush @12:08:48.58 CS.seek 5ml @12:09:01.47 delay 5 seconds @12:09:06.50 CSV.dial :air @12:09:07.07 CS.seek 7ml @12:09:12.54 AFV.select :carrierSolution @12:09:12.64 delay 2 seconds @12:09:12.81 CSV.dial CTV @12:09:12.84
CTV.dial CSV @12:09:13.32 CBV.dial :waste2b @12:09:14.16 CS.seek 0ml @12:09:14.67 AS.reconfigure ASconfig @12:09:14.72 AS.seek 1ml @12:09:31.78
Flushing upper manifold @12:09:31.92 CSV.dial :flush @12:09:33.37 CS.seek 5ml @12:09:46.18 delay 5 seconds @12:09:51.21 CSV.dial :air @12:09:51.80 CS.seek 7ml @12:09:57.53 CSV.dial CBV @12:09:57.56
CBV.dial CSV @12:09:58.75 CTV.dial :air @12:10:00.20 CS.seek 0ml @12:10:02.77 delay 1 second @12:10:03.79 AFV.select :holdingCoil @12:10:03.89 delay 1 second @12:10:04.92 ASV.dial :wasteD @12:10:05.92 AS.seek 0ml @12:10:17.65
delay 7 seconds @12:10:24.69 CS.seek 3ml @12:10:32.94 CSV.dial :waste @12:10:33.45 CS.seek 0ml @12:10:41.66 CSV.dial :flush @12:10:42.15 CS.seek 3ml @12:10:50.37 delay 5 seconds @12:10:55.40 CSV.dial :air @12:10:55.94 CS.seek 5ml @12:11:01.75 CSV.dial :air @12:11:01.77 CS.seek 0ml @12:11:03.68 Priming Eluate @12:11:03.69 AFV.select :carrierSolution @12:11:03.80 delay 2 seconds @12:11:05.84 AS.seek 0.5ml @12:11:14.50
delay 7 seconds @12:11:21.54 CS.seek 3ml @12:11:29.56 CSV.dial :waste @12:11:30.35 CS.seek 0ml @12:11:34.88 delay 1 second @12:11:35.90 AFV.select :holdingCoil @12:11:36.00 ATV.dial ASV @12:11:36.01 ASV.dial :SPErecovery @12:11:36.58 Swish #1 of 2 @12:11:36.66 AS.reconfigure ASslow0 @12:11:36.71 AS.seek 0.6ml @12:11:38.34
Disinfecting external Intake with mfbkill @12:11:38.48 CSV.dial CSR @12:11:39.95 Pulling 8ml of CSR.mfbkill @12:11:39.96 CSR.select :mfbkill @12:11:40.08 CS.seek 8ml @12:11:47.17 AS.seek 0.5ml @12:11:57.54 Swish #2 of 2 @12:11:57.63 AS.seek 0.6ml @12:11:59.63
delay 5 seconds @12:12:04.66 CSR.select :air @12:12:04.78 CS.seek 10ml @12:12:08.12 AS.seek 0.5ml @12:12:10.40 CSV.dial CBV @12:12:10.42
CBV.dial CSV @12:12:11.52 CTV.dial :intake @12:12:12.33 Intake.select :open @12:12:12.45 CS.seek 2ml @12:12:18.48 delay 3 seconds @12:12:21.57 AS.reconfigure ASslow1 @12:12:21.62 AS.seek 0.684ml @12:12:33.13
Intake.select :close @12:12:33.24 CTV.dial :air @12:12:34.05 CS.reconfigure CSconfig @12:12:34.10 CS.seek 0ml @12:12:40.22 Flushing lower manifold's waste1 port @12:12:40.23 CSV.dial CSR @12:12:41.38 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @12:12:41.51 CS.seek 4ml @12:12:52.72 delay 5 seconds @12:12:57.75 CSR.select :air @12:12:57.88 CS.reconfigure CSfast @12:12:57.92 CS.seek 10ml @12:12:58.95 delay 3 seconds @12:13:01.97 Eluate primed @12:13:01.98 ATV.dial :waste2 @12:13:02.57 AS.reconfigure ASnorm @12:13:02.62 AS.seek 0ml @12:13:12.85 CSV.dial CTV @12:13:12.87
CTV.dial CSV @12:13:14.91 CBV.dial :waste1 @12:13:15.51 CS.seek 0ml @12:13:37.45 SPE.coast @12:13:37.53 ASV.dial :SPErecovery @12:13:37.55 Setting up PCR for OM -- writing /var/log/esp/OM20may1912h450ml.pcr @12:13:37.57 Building PCR Slug from ASV with MM3 mix, PP5 primer @12:13:37.58 Priming with carrier @12:13:37.59 ATV.dial ARV @12:13:38.52 ARV.dial :carrier @12:13:38.86 AS.seek 0.5ml @12:13:39.47
Flushing lower manifold's waste2b port @12:13:39.48 CSV.dial CSR @12:13:40.02 Pulling 4ml of CSR.mfbkill @12:13:40.03 CSR.select :mfbkill @12:13:40.15 CS.reconfigure CSconfig @12:13:40.20 CS.seek 4ml @12:13:51.35 delay 5 seconds @12:13:56.38 CSR.select :air @12:13:56.51 CS.reconfigure CSfast @12:13:56.55 CS.seek 10ml @12:14:04.41 AFV.select :carrierSolution @12:14:04.52 delay 2 seconds @12:14:06.55 AS.reconfigure ASconfig @12:14:06.60 AS.seek 1ml @12:14:11.07 CSV.dial CTV @12:14:11.11
CTV.dial CSV @12:14:11.61 CBV.dial :waste2b @12:14:12.43 CS.seek 0ml @12:14:35.68 delay 1 second @12:14:35.97
Killing upper manifold @12:14:35.98 CSV.dial CSR @12:14:36.53 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @12:14:36.66 CS.reconfigure CSconfig @12:14:36.71 AFV.select :holdingCoil @12:14:36.73
CS.seek 4ml @12:14:36.90 delay 1 second @12:14:37.93 ATV.dial :waste @12:14:39.34 AS.reconfigure ASnorm @12:14:39.40 AS.seek :empty @12:14:47.97
delay 5 seconds @12:14:53.00 CSR.select :air @12:14:53.13 CS.reconfigure CSfast @12:14:53.17 CS.seek 10ml @12:15:07.69 CSV.dial CBV @12:15:07.72
CBV.dial CSV @12:15:08.86 CTV.dial :air @12:15:10.23 CS.seek 0ml @12:15:30.13 AFV.select :carrierSolution @12:15:30.24 delay 2 seconds @12:15:32.27 AS.reconfigure ASconfig @12:15:32.32 AS.seek 0.5ml @12:15:33.66
delay 7 seconds @12:15:40.70 CS.seek 3ml @12:15:48.54 CSV.dial :waste @12:15:49.01 CS.seek 0ml @12:15:56.85 Flushing upper manifold @12:15:56.99 CSV.dial :flush @12:15:57.45 CS.seek 5ml @12:16:01.40 delay 1 second @12:16:02.42 AFV.select :holdingCoil @12:16:02.52 Building Slug @12:16:02.53 ATV.dial :ARV @12:16:04.07 delay 2 seconds @12:16:06.10 ARV.dial :air1 @12:16:06.48 delay 2 seconds @12:16:08.57 AS.reconfigure ASslow2 @12:16:08.63 AS.seek 0.506ml @12:16:09.76
delay 5 seconds @12:16:14.78 CSV.dial :air @12:16:14.90 delay 2 seconds @12:16:15.31
CS.seek 7ml @12:16:16.93 ARV.dial :MM3 @12:16:17.95 delay 2 seconds @12:16:19.98 Adding MM3 @12:16:20.06 AS.seek 0.524ml @12:16:21.07 CSV.dial CBV @12:16:21.11
CBV.dial CSV @12:16:22.18 CTV.dial :air @12:16:22.20 CS.seek 0ml @12:16:38.31 delay 25 seconds @12:16:38.91
delay 7 seconds @12:16:45.95 CS.seek 3ml @12:16:53.94 CSV.dial :waste @12:16:55.19 CS.seek 0ml @12:17:03.17 CSV.dial :flush @12:17:03.33 ARV.dial :PP5 @12:17:03.66
CS.seek 3ml @12:17:03.74 Adding PP5 @12:17:03.76 delay 2 seconds @12:17:05.85 AS.seek 0.53ml @12:17:11.60
delay 5 seconds @12:17:12.09 delay 10 seconds @12:17:16.63
CSV.dial :air @12:17:17.17 CS.seek 5.001ml @12:17:22.12 ARV.dial :air3 @12:17:22.51 adding ARV air @12:17:22.53 delay 2 seconds @12:17:22.66
CSV.dial :air @12:17:22.68 CS.seek 0ml @12:17:24.61 AS.seek 0.536ml @12:17:30.82 delay 5 seconds @12:17:34.91
delay 7 seconds @12:17:35.84 ARV.dial :carrier @12:17:37.07 delay 7 seconds @12:17:41.95
CS.seek 3ml @12:17:44.17 AS.seek 0.594ml @12:17:49.88
CSV.dial :waste @12:17:50.66 CS.seek 0ml @12:17:58.51 Flushing lower manifold's waste1 port @12:17:58.65 CSV.dial :flush @12:17:59.14 CS.seek 5ml @12:18:11.41 delay 5 seconds @12:18:16.44 CSV.dial :air @12:18:17.11 CS.seek 7ml @12:18:22.57 CSV.dial CTV @12:18:22.60
CTV.dial CSV @12:18:24.05 CBV.dial :waste1 @12:18:24.55 CS.seek 0ml @12:18:41.20 Flushing lower manifold's waste2b port @12:18:41.34 CSV.dial :flush @12:18:42.13 CS.seek 5ml @12:18:42.70 delay 5 seconds @12:18:47.73 ATV.dial :ASV @12:18:48.12 delay 2 seconds @12:18:50.21 AS.seek 0.6ml @12:18:54.41
delay 5 seconds @12:18:56.82 delay 4 seconds @12:18:59.43
CSV.dial :air @12:18:59.98 CS.seek 7ml @12:19:00.84 ATV.dial ARV @12:19:01.48 delay 2 seconds @12:19:03.50 ARV.dial :carrier @12:19:03.51 delay 2 seconds @12:19:05.59 CSV.dial CTV @12:19:05.64
CTV.dial CSV @12:19:05.72 AS.seek 0.65ml @12:19:06.10
CBV.dial :waste2b @12:19:06.92 CS.seek 0ml @12:19:23.63 Disinfecting external Intake with kill @12:19:23.77 CSV.dial CSR @12:19:24.29 Pulling 8ml of CSR.kill @12:19:24.30 CSR.select :kill @12:19:24.43 CS.seek 8ml @12:19:43.48 delay 5 seconds @12:19:48.51 CSR.select :air @12:19:48.64 CS.seek 10ml @12:19:54.21 CSV.dial CBV @12:19:54.24
CBV.dial CSV @12:19:55.37 CTV.dial :intake @12:19:56.17 Intake.select :open @12:19:56.29 CS.seek 2ml @12:19:56.36 delay 4 seconds @12:20:00.39 ATV.dial PCR @12:20:01.73 ASV.dial :waste1 @12:20:02.50 ARV.dial :air1 @12:20:02.87 Positioning Slug by 0.324ml @12:20:02.96 AS.seek 0.326ml @12:20:16.85
Intake.select :close @12:20:16.96 CTV.dial :air @12:20:17.74 CS.reconfigure CSconfig @12:20:17.79 CS.seek 0ml @12:20:23.98 CSV.dial CSR @12:20:25.13 Pulling 5ml of CSR.flush @12:20:25.14 CSR.select :flush @12:20:25.26 CS.seek 5ml @12:20:38.98 delay 5 seconds @12:20:44.01 CSR.select :air @12:20:44.14 CS.seek 10ml @12:20:57.98 CSV.dial CBV @12:20:59.04 CS.seek 0ml @12:21:25.57 Garaging FlushPuck in Collection Clamp @12:21:25.65 Elbow.reconfigure TightElbow @12:21:25.70 Elbow.seek CC @12:21:25.94 Elbow.reconfigure LooseElbow @12:21:26.04 Forearm.seek CC @12:21:27.06 Hand.close @12:21:27.71 CC.seek :open @12:21:32.08 Forearm.seek :retract @12:21:33.10 Elbow.reconfigure TightElbow @12:21:33.15 Elbow.seek :garage @12:21:36.35 Elbow.reconfigure LooseElbow @12:21:36.46 Forearm.reconfigure TightForearm @12:21:36.51 Forearm.seek :garage @12:21:37.68 Hand.open @12:21:38.39 Forearm.reconfigure LooseForearm @12:21:38.44 Forearm.seek :retract @12:21:39.46 Starting initialPurge @12:21:39.47 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:21:39.55 Hand.open @12:21:39.89 Elbow.reconfigure TightElbow @12:21:39.94 Elbow.seek FlushPuck::Garage @12:21:40.07 Elbow.reconfigure LooseElbow @12:21:40.18 Forearm.seek :garage @12:21:41.20 Hand.close @12:21:41.95 Forearm.seek :retract @12:21:42.96 Elbow.reconfigure TightElbow @12:21:43.01 Elbow.seek CC @12:21:45.93 Elbow.reconfigure LooseElbow @12:21:46.04 Forearm.reconfigure TightForearm @12:21:46.09 Forearm.seek CC @12:21:47.39 CC.seek :closed @12:21:51.67 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.98 @12:21:51.75 Hand.open @12:21:52.39 Forearm.reconfigure LooseForearm @12:21:52.44 Forearm.seek :retract @12:21:53.50 Flushing the Collection Valve::Manifold @12:21:54.30 CSV.dial CSR @12:21:55.42 Pulling 5ml of CSR.flush @12:21:55.43 CSR.select :flush @12:21:55.56 CS.seek 5ml @12:22:09.36 delay 5 seconds @12:22:14.39 CSR.select :air @12:22:14.57 CS.reconfigure CSfast @12:22:14.62 CS.seek 7.5ml @12:22:21.23 CSV.dial :waste @12:22:22.57 CS.reconfigure CSconfig @12:22:22.62 CS.seek 0ml @12:22:42.76 Purging external Intake @12:22:42.90 CSV.dial :flush @12:22:43.37 CS.reconfigure CSfast @12:22:43.42 CS.seek 10ml @12:23:06.89 delay 5 seconds @12:23:11.92 CSV.dial CBV @12:23:11.96
CBV.dial CSV @12:23:12.75 CTV.dial :intake @12:23:13.54 Intake.select :open @12:23:13.66 CS.seek 0ml @12:23:38.61 Intake.select :close @12:23:38.72 CTV.dial :air @12:23:39.51 Flushing upper manifold @12:23:39.65 CSV.dial :flush @12:23:40.43 CS.seek 5ml @12:23:52.64 delay 5 seconds @12:23:57.67 CSV.dial :air @12:23:58.21 CS.seek 7ml @12:24:03.73 CSV.dial CBV @12:24:03.77
CBV.dial CSV @12:24:04.84 CTV.dial :air @12:24:04.86 CS.seek 0ml @12:24:21.55 delay 7 seconds @12:24:28.59 CS.seek 3ml @12:24:36.33 CSV.dial :waste @12:24:36.82 CS.seek 0ml @12:24:44.76 CSV.dial :flush @12:24:45.23 CS.seek 3ml @12:24:53.05 delay 5 seconds @12:24:58.08 CSV.dial :air @12:24:58.62 CS.seek 5ml @12:25:04.27 CSV.dial :air @12:25:04.29 CS.seek 0ml @12:25:16.48 delay 7 seconds @12:25:23.52 CS.seek 3ml @12:25:28.81 delay 5 seconds @12:25:31.36
CSV.dial :waste @12:25:32.13 CS.seek 0ml @12:25:33.84 ATV.dial ARV @12:25:34.62 ASV.dial :PCRlock @12:25:34.98 delay 5 seconds @12:25:39.95
Garaging FlushPuck in Collection Clamp @12:25:40.03 Elbow.reconfigure TightElbow @12:25:40.08 Elbow.seek CC @12:25:40.11 Running OM Profile60C350 thermocycling @12:25:40.17 PCR.program Profile60C350 @12:25:40.28 Profile60C350 should complete in < 2:02:45 @12:25:40.33 PCR.run 2:38:26.25 @12:25:40.45
Elbow.reconfigure LooseElbow @12:25:40.55 Forearm.seek CC @12:25:41.55 Hand.close @12:25:42.21 CC.seek :open @12:25:46.61 Forearm.seek :retract @12:25:47.61 Elbow.reconfigure TightElbow @12:25:47.66 Elbow.seek :garage @12:25:50.81 Elbow.reconfigure LooseElbow @12:25:50.92 Forearm.reconfigure TightForearm @12:25:50.97 Forearm.seek :garage @12:25:52.11 Hand.open @12:25:52.78 Forearm.reconfigure LooseForearm @12:25:52.83 Forearm.seek :retract @12:25:53.83 Completed [stdphase] begun 10:02:01 @12:25:54.49 Preparing to sleep until 10:00 20-may-20 @12:25:54.54 deferred until MFBPCR finish @12:25:54.57
Waiting for MFBPCR til 10:00 20-may-20 @12:25:54.60 Continuing at 10:00CEST20-May-20 @12:25:54.66 GoodNews.email "Then, in tube 2: wcrphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:129\n{1 => 21, 2 => 1}\nCan@12:25:54, 9.5C, 45% humidity, 14.4psia, 13.466V, 0.897A, 0.897A avg, 12.08W",Subject: "Waiting until 10:00CEST20-May-20" @14:02:22.47 PCR.off @14:02:23.74 PCR for OM completed -- closed /var/log/esp/OM20may1912h450ml.pcr @14:02:23.81 Cleaning PCR @14:02:23.82 AFV.select :carrierSolution @14:02:23.93 delay 2 seconds @14:02:25.96 AS.reconfigure ASconfig @14:02:26.01 AS.seek 0.5ml @14:02:36.32 delay 1 second @14:02:37.34 AFV.select :holdingCoil @14:02:37.44 ASV.dial :waste1 @14:02:38.50 ATV.dial :air3 @14:02:39.72 AS.reconfigure ASslow1 @14:02:39.77 AS.seek 0.51ml @14:02:42.04 delay 2 seconds @14:02:44.06 ATV.dial :bleach @14:02:44.66 AS.seek 0.81ml @14:03:45.23 delay 3 seconds @14:03:48.26 ATV.dial :air3 @14:03:48.79 delay 3 seconds @14:03:51.88 AS.seek 0.835ml @14:03:57.16 delay 3 seconds @14:04:00.19 ATV.dial :bleach @14:04:00.80 AS.seek 0.82ml @14:04:04.09 delay 3 seconds @14:04:07.12 ARV.dial :waste @14:04:07.70 delay 3 seconds @14:04:10.73 ATV.dial :ARV @14:04:11.69 AS.reconfigure ASconfig @14:04:11.74 AS.seek 0.47ml @14:04:32.15 AFV.select :carrierSolution @14:04:32.26 delay 2 seconds @14:04:34.30 AS.seek 1ml @14:05:05.12 delay 1 second @14:05:06.14 AFV.select :holdingCoil @14:05:06.24 delay 1 second @14:05:07.27 AS.reconfigure ASfast @14:05:07.32 AS.seek 0ml @14:05:34.66 AFV.select :carrierSolution @14:05:34.77 delay 2 seconds @14:05:36.80 AS.reconfigure ASconfig @14:05:36.85 AS.seek 1ml @14:06:34.61 delay 1 second @14:06:35.63 AFV.select :holdingCoil @14:06:35.73 delay 1 second @14:06:36.76 AS.reconfigure ASfast @14:06:36.81 AS.seek 0ml @14:07:04.14 AFV.select :carrierSolution @14:07:04.25 delay 2 seconds @14:07:06.28 AS.reconfigure ASconfig @14:07:06.33 AS.seek 1ml @14:08:04.11 delay 1 second @14:08:05.13 AFV.select :holdingCoil @14:08:05.23 delay 1 second @14:08:06.26 AS.reconfigure ASfast @14:08:06.31 AS.seek 0ml @14:08:33.63 ARV.dial between carrier and air1 @14:08:34.11 ATV.dial :air3 @14:08:35.34 AS.reconfigure ASslow1 @14:08:35.39 AS.seek 0.01ml @14:08:37.69 delay 2 seconds @14:08:39.71 ATV.dial :bleach @14:08:40.32 AS.seek 0.81ml @14:11:21.46 delay 3 seconds @14:11:24.49 ATV.dial :air3 @14:11:25.02 delay 3 seconds @14:11:28.11 AS.seek 0.835ml @14:11:33.42 delay 3 seconds @14:11:36.45 ATV.dial :bleach @14:11:37.06 AS.seek 0.82ml @14:11:40.38 delay 3 seconds @14:11:43.41 ATV.dial PCR @14:11:43.80 AS.reconfigure ASconfig @14:11:43.85 AS.seek :empty @14:12:31.32 PCR.program :bleachProfile @14:12:31.38 PCR program should complete in < 2:45 @14:12:31.39 PCR.run 10:00 @14:15:45.32 PCR.off @14:15:45.35 AFV.select :carrierSolution @14:15:45.46 delay 2 seconds @14:15:47.50 AS.seek 1ml @14:16:45.32 delay 1 second @14:16:46.34 AFV.select :holdingCoil @14:16:46.44 delay 1 second @14:16:47.47 AS.reconfigure ASfast @14:16:47.52 AS.seek 0ml @14:17:14.88 delay 3 seconds @14:17:17.91 AFV.select :carrierSolution @14:17:18.01 delay 2 seconds @14:17:20.04 AS.reconfigure ASconfig @14:17:20.10 AS.seek 1ml @14:18:17.93 delay 1 second @14:18:18.95 AFV.select :holdingCoil @14:18:19.05 delay 1 second @14:18:20.08 AS.reconfigure ASfast @14:18:20.13 AS.seek 0ml @14:18:47.48 delay 3 seconds @14:18:51.16 AFV.select :carrierSolution @14:18:51.26 delay 2 seconds @14:18:53.29 AS.reconfigure ASconfig @14:18:53.35 AS.seek 1ml @14:19:51.16 delay 1 second @14:19:52.18 AFV.select :holdingCoil @14:19:52.28 delay 1 second @14:19:53.31 AS.reconfigure ASfast @14:19:53.36 AS.seek 0ml @14:20:20.68 delay 3 seconds @14:20:23.71 AFV.select :carrierSolution @14:20:23.81 delay 2 seconds @14:20:25.84 AS.reconfigure ASconfig @14:20:25.89 AS.seek 1ml @14:21:23.70 delay 1 second @14:21:24.72 AFV.select :holdingCoil @14:21:24.82 delay 1 second @14:21:25.85 AS.reconfigure ASfast @14:21:25.90 AS.seek 0ml @14:21:53.23 delay 3 seconds @14:21:56.26 AFV.select :carrierSolution @14:21:56.36 delay 2 seconds @14:21:58.40 AS.reconfigure ASconfig @14:21:58.45 AS.seek 1ml @14:22:56.26 delay 1 second @14:22:57.28 AFV.select :holdingCoil @14:22:57.38 delay 1 second @14:22:58.41 AS.reconfigure ASnorm @14:22:58.46 AS.seek 0ml @14:23:49.22 Setting up PCR for ML -- writing /var/log/esp/ML20may1914h450ml.pcr @14:23:49.24 Building PCR Slug from ASV with MM2 mix, PP4 primer @14:23:49.26 Priming with carrier @14:23:49.27 ATV.dial ARV @14:23:49.86 ARV.dial :carrier @14:23:50.21 AS.seek 0.5ml @14:24:15.76 AFV.select :carrierSolution @14:24:15.87 delay 2 seconds @14:24:17.90 AS.reconfigure ASconfig @14:24:17.95 AS.seek 1ml @14:24:47.00 delay 1 second @14:24:48.02 AFV.select :holdingCoil @14:24:48.12 delay 1 second @14:24:49.15 ATV.dial :waste @14:24:50.72 AS.reconfigure ASnorm @14:24:50.78 AS.seek :empty @14:25:41.53 AFV.select :carrierSolution @14:25:41.64 delay 2 seconds @14:25:43.67 AS.reconfigure ASconfig @14:25:43.72 AS.seek 0.5ml @14:26:12.79 delay 1 second @14:26:13.81 AFV.select :holdingCoil @14:26:13.91 Building Slug @14:26:13.92 ATV.dial :ARV @14:26:15.47 delay 2 seconds @14:26:17.50 ARV.dial :air1 @14:26:17.90 delay 2 seconds @14:26:19.99 AS.reconfigure ASslow2 @14:26:20.04 AS.seek 0.506ml @14:26:26.25 delay 2 seconds @14:26:28.28 ARV.dial :MM2 @14:26:29.67 delay 2 seconds @14:26:31.70 Adding MM2 @14:26:31.77 AS.seek 0.524ml @14:26:50.06 delay 25 seconds @14:27:15.09 ARV.dial :PP4 @14:27:15.47 Adding PP4 @14:27:15.49 delay 2 seconds @14:27:17.57 AS.seek 0.53ml @14:27:23.80 delay 10 seconds @14:27:33.83 ARV.dial :air3 @14:27:34.22 adding ARV air @14:27:34.24 delay 2 seconds @14:27:36.32 AS.seek 0.536ml @14:27:42.53 delay 5 seconds @14:27:47.56 ARV.dial :carrier @14:27:48.76 delay 7 seconds @14:27:55.85 AS.seek 0.594ml @14:28:54.44 delay 5 seconds @14:28:59.47 ATV.dial :ASV @14:28:59.86 delay 2 seconds @14:29:01.95 AS.seek 0.6ml @14:29:08.34 delay 4 seconds @14:29:12.37 ATV.dial ARV @14:29:13.00 delay 2 seconds @14:29:15.03 ARV.dial :carrier @14:29:15.04 delay 2 seconds @14:29:17.13 AS.seek 0.65ml @14:30:07.55 delay 4 seconds @14:30:11.58 ATV.dial PCR @14:30:12.16 ASV.dial :waste1 @14:30:12.17 ARV.dial :air1 @14:30:12.57 Positioning Slug by 0.324ml @14:30:12.66 AS.seek 0.326ml @14:35:38.72 delay 5 seconds @14:35:43.75 ATV.dial ARV @14:35:44.49 ASV.dial :PCRlock @14:35:44.88 delay 5 seconds @14:35:49.91 Running ML Profile60C350 thermocycling @14:35:49.92 PCR.program Profile60C350 @14:35:50.68 Profile60C350 should complete in < 2:02:45 @14:35:50.70 PCR.run 2:38:26.25 @16:12:07.28 PCR.off @16:12:08.55 PCR for ML completed -- closed /var/log/esp/ML20may1914h450ml.pcr @16:12:08.62 Cleaning PCR @16:12:08.63 AFV.select :carrierSolution @16:12:08.74 delay 2 seconds @16:12:10.77 AS.reconfigure ASconfig @16:12:10.82 AS.seek 0.5ml @16:12:21.15 delay 1 second @16:12:22.17 AFV.select :holdingCoil @16:12:22.27 ASV.dial :waste1 @16:12:22.65 ATV.dial :air3 @16:12:23.88 AS.reconfigure ASslow1 @16:12:23.93 AS.seek 0.51ml @16:12:26.21 delay 2 seconds @16:12:28.23 ATV.dial :bleach @16:12:28.83 AS.seek 0.81ml @16:13:29.43 delay 3 seconds @16:13:32.46 ATV.dial :air3 @16:13:32.99 delay 3 seconds @16:13:36.08 AS.seek 0.835ml @16:13:41.35 delay 3 seconds @16:13:44.38 ATV.dial :bleach @16:13:44.99 AS.seek 0.82ml @16:13:48.28 delay 3 seconds @16:13:51.30 ARV.dial :waste @16:13:51.89 delay 3 seconds @16:13:54.92 ATV.dial :ARV @16:13:55.88 AS.reconfigure ASconfig @16:13:55.94 AS.seek 0.47ml @16:14:16.34 AFV.select :carrierSolution @16:14:16.46 delay 2 seconds @16:14:18.50 AS.seek 1ml @16:14:49.26 delay 1 second @16:14:50.28 AFV.select :holdingCoil @16:14:50.98 delay 1 second @16:14:52.02 AS.reconfigure ASfast @16:14:52.07 AS.seek 0ml @16:15:19.42 AFV.select :carrierSolution @16:15:19.53 delay 2 seconds @16:15:21.56 AS.reconfigure ASconfig @16:15:21.61 AS.seek 1ml @16:16:19.37 delay 1 second @16:16:20.39 AFV.select :holdingCoil @16:16:20.49 delay 1 second @16:16:21.52 AS.reconfigure ASfast @16:16:21.57 AS.seek 0ml @16:16:48.93 AFV.select :carrierSolution @16:16:49.04 delay 2 seconds @16:16:51.07 AS.reconfigure ASconfig @16:16:51.12 AS.seek 1ml @16:17:48.90 delay 1 second @16:17:49.92 AFV.select :holdingCoil @16:17:50.02 delay 1 second @16:17:51.05 AS.reconfigure ASfast @16:17:51.10 AS.seek 0ml @16:18:18.43 ARV.dial between carrier and air1 @16:18:18.90 ATV.dial :air3 @16:18:20.13 AS.reconfigure ASslow1 @16:18:20.18 AS.seek 0.01ml @16:18:22.47 delay 2 seconds @16:18:24.49 ATV.dial :bleach @16:18:25.10 AS.seek 0.81ml @16:21:06.25 delay 3 seconds @16:21:09.28 ATV.dial :air3 @16:21:09.81 delay 3 seconds @16:21:12.90 AS.seek 0.835ml @16:21:18.21 delay 3 seconds @16:21:21.24 ATV.dial :bleach @16:21:21.85 AS.seek 0.82ml @16:21:25.16 delay 3 seconds @16:21:28.19 ATV.dial PCR @16:21:28.59 AS.reconfigure ASconfig @16:21:28.64 AS.seek :empty @16:22:16.09 PCR.program :bleachProfile @16:22:16.14 PCR program should complete in < 2:45 @16:22:16.15 PCR.run 10:00 @16:25:30.08 PCR.off @16:25:30.12 AFV.select :carrierSolution @16:25:30.23 delay 2 seconds @16:25:32.27 AS.seek 1ml @16:26:30.10 delay 1 second @16:26:31.12 AFV.select :holdingCoil @16:26:31.22 delay 1 second @16:26:32.25 AS.reconfigure ASfast @16:26:32.30 AS.seek 0ml @16:26:59.63 delay 3 seconds @16:27:02.66 AFV.select :carrierSolution @16:27:02.76 delay 2 seconds @16:27:04.79 AS.reconfigure ASconfig @16:27:04.85 AS.seek 1ml @16:28:02.61 delay 1 second @16:28:03.63 AFV.select :holdingCoil @16:28:03.73 delay 1 second @16:28:04.76 AS.reconfigure ASfast @16:28:04.81 AS.seek 0ml @16:28:32.13 delay 3 seconds @16:28:35.16 AFV.select :carrierSolution @16:28:35.26 delay 2 seconds @16:28:37.29 AS.reconfigure ASconfig @16:28:37.34 AS.seek 1ml @16:29:35.11 delay 1 second @16:29:36.13 AFV.select :holdingCoil @16:29:36.23 delay 1 second @16:29:37.26 AS.reconfigure ASfast @16:29:37.31 AS.seek 0ml @16:30:04.63 delay 3 seconds @16:30:07.66 AFV.select :carrierSolution @16:30:07.76 delay 2 seconds @16:30:09.79 AS.reconfigure ASconfig @16:30:09.85 AS.seek 1ml @16:31:07.63 delay 1 second @16:31:08.65 AFV.select :holdingCoil @16:31:08.75 delay 1 second @16:31:09.78 AS.reconfigure ASfast @16:31:09.83 AS.seek 0ml @16:31:37.18 delay 3 seconds @16:31:40.21 AFV.select :carrierSolution @16:31:40.31 delay 2 seconds @16:31:42.34 AS.reconfigure ASconfig @16:31:42.40 AS.seek 1ml @16:32:40.21 delay 1 second @16:32:41.23 AFV.select :holdingCoil @16:32:41.33 delay 1 second @16:32:42.36 AS.reconfigure ASnorm @16:32:42.41 AS.seek 0ml @16:33:33.12 Setting up PCR for CG -- writing /var/log/esp/CG20may1916h450ml.pcr @16:33:33.13 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:33:33.15 Priming with carrier @16:33:33.16 ATV.dial ARV @16:33:33.74 ARV.dial :carrier @16:33:34.08 AS.seek 0.5ml @16:33:59.59 AFV.select :carrierSolution @16:33:59.70 delay 2 seconds @16:34:02.38 AS.reconfigure ASconfig @16:34:02.44 AS.seek 1ml @16:34:31.51 delay 1 second @16:34:32.53 AFV.select :holdingCoil @16:34:32.63 delay 1 second @16:34:33.66 ATV.dial :waste @16:34:35.23 AS.reconfigure ASnorm @16:34:35.29 AS.seek :empty @16:35:26.06 AFV.select :carrierSolution @16:35:26.17 delay 2 seconds @16:35:28.20 AS.reconfigure ASconfig @16:35:28.25 AS.seek 0.5ml @16:35:57.31 delay 1 second @16:35:58.33 AFV.select :holdingCoil @16:35:58.43 Building Slug @16:35:58.44 ATV.dial :ARV @16:36:00.09 delay 2 seconds @16:36:02.12 ARV.dial :air1 @16:36:02.49 delay 2 seconds @16:36:04.58 AS.reconfigure ASslow2 @16:36:04.63 AS.seek 0.506ml @16:36:10.81 delay 2 seconds @16:36:12.84 ARV.dial :MM1 @16:36:13.42 delay 2 seconds @16:36:15.45 Adding MM1 @16:36:15.52 AS.seek 0.524ml @16:36:33.90 delay 25 seconds @16:36:58.93 ARV.dial :PP2 @16:36:59.31 Adding PP2 @16:36:59.33 delay 2 seconds @16:37:01.41 AS.seek 0.53ml @16:37:07.84 delay 10 seconds @16:37:17.87 ARV.dial :air2 @16:37:18.25 adding ARV air @16:37:18.27 delay 2 seconds @16:37:20.35 AS.seek 0.536ml @16:37:26.56 delay 5 seconds @16:37:31.59 ARV.dial :carrier @16:37:32.79 delay 7 seconds @16:37:39.88 AS.seek 0.594ml @16:38:38.46 delay 5 seconds @16:38:43.49 ATV.dial :ASV @16:38:43.89 delay 2 seconds @16:38:45.98 AS.seek 0.6ml @16:38:52.34 delay 4 seconds @16:38:56.37 ATV.dial ARV @16:38:57.00 delay 2 seconds @16:38:59.03 ARV.dial :carrier @16:38:59.04 delay 2 seconds @16:39:01.13 AS.seek 0.65ml @16:39:51.62 delay 4 seconds @16:39:55.65 ATV.dial PCR @16:39:56.34 ASV.dial :waste1 @16:39:56.35 ARV.dial :air1 @16:39:56.74 Positioning Slug by 0.324ml @16:39:56.83 AS.seek 0.326ml @16:45:22.89 delay 5 seconds @16:45:27.92 ATV.dial ARV @16:45:28.66 ASV.dial :PCRlock @16:45:29.05 delay 5 seconds @16:45:34.08 Running CG Profile60C300 thermocycling @16:45:34.09 PCR.program Profile60C300 @16:45:34.20 Profile60C300 should complete in < 1:59:25 @16:45:34.21 PCR.run 2:34:16.25 @18:20:52.21 PCR.off @18:20:52.98 PCR for CG completed -- closed /var/log/esp/CG20may1916h450ml.pcr @18:20:53.06 Cleaning PCR @18:20:53.07 AFV.select :carrierSolution @18:20:53.18 delay 2 seconds @18:20:55.26 AS.reconfigure ASconfig @18:20:55.30 AS.seek 0.5ml @18:21:05.61 delay 1 second @18:21:06.63 AFV.select :holdingCoil @18:21:06.73 ASV.dial :waste1 @18:21:07.11 ATV.dial :air3 @18:21:08.33 AS.reconfigure ASslow1 @18:21:08.38 AS.seek 0.51ml @18:21:11.42 delay 2 seconds @18:21:13.44 ATV.dial :bleach @18:21:14.04 AS.seek 0.81ml @18:22:14.63 delay 3 seconds @18:22:17.66 ATV.dial :air3 @18:22:18.22 delay 3 seconds @18:22:21.31 AS.seek 0.835ml @18:22:26.58 delay 3 seconds @18:22:29.61 ATV.dial :bleach @18:22:30.22 AS.seek 0.82ml @18:22:33.48 delay 3 seconds @18:22:36.50 ARV.dial :waste @18:22:37.08 delay 3 seconds @18:22:40.11 ATV.dial :ARV @18:22:41.07 AS.reconfigure ASconfig @18:22:41.13 AS.seek 0.47ml @18:23:01.51 AFV.select :carrierSolution @18:23:01.63 delay 2 seconds @18:23:03.67 AS.seek 1ml @18:23:34.44 delay 1 second @18:23:35.46 AFV.select :holdingCoil @18:23:35.56 delay 1 second @18:23:36.59 AS.reconfigure ASfast @18:23:36.64 AS.seek 0ml @18:24:03.96 AFV.select :carrierSolution @18:24:04.07 delay 2 seconds @18:24:06.10 AS.reconfigure ASconfig @18:24:06.15 AS.seek 1ml @18:25:03.90 delay 1 second @18:25:04.92 AFV.select :holdingCoil @18:25:05.02 delay 1 second @18:25:06.05 AS.reconfigure ASfast @18:25:06.10 AS.seek 0ml @18:25:33.43 AFV.select :carrierSolution @18:25:33.54 delay 2 seconds @18:25:35.57 AS.reconfigure ASconfig @18:25:35.62 AS.seek 1ml @18:26:33.40 delay 1 second @18:26:34.42 AFV.select :holdingCoil @18:26:34.52 delay 1 second @18:26:35.55 AS.reconfigure ASfast @18:26:35.60 AS.seek 0ml @18:27:02.93 ARV.dial between carrier and air1 @18:27:03.42 ATV.dial :air3 @18:27:04.64 AS.reconfigure ASslow1 @18:27:04.69 AS.seek 0.01ml @18:27:06.98 delay 2 seconds @18:27:09.00 ATV.dial :bleach @18:27:09.61 AS.seek 0.81ml @18:29:50.75 delay 3 seconds @18:29:53.77 ATV.dial :air3 @18:29:54.31 delay 3 seconds @18:29:57.40 AS.seek 0.835ml @18:30:02.68 delay 3 seconds @18:30:05.71 ATV.dial :bleach @18:30:06.32 AS.seek 0.82ml @18:30:09.61 delay 3 seconds @18:30:12.63 ATV.dial PCR @18:30:13.03 AS.reconfigure ASconfig @18:30:13.08 AS.seek :empty @18:31:00.53 PCR.program :bleachProfile @18:31:00.59 PCR program should complete in < 2:45 @18:31:00.60 PCR.run 10:00 @18:34:15.20 PCR.off @18:34:15.22 AFV.select :carrierSolution @18:34:15.33 delay 2 seconds @18:34:17.37 AS.seek 1ml @18:35:15.23 delay 1 second @18:35:16.25 AFV.select :holdingCoil @18:35:16.35 delay 1 second @18:35:17.38 AS.reconfigure ASfast @18:35:17.43 AS.seek 0ml @18:35:44.77 delay 3 seconds @18:35:47.80 AFV.select :carrierSolution @18:35:47.90 delay 2 seconds @18:35:49.93 AS.reconfigure ASconfig @18:35:49.99 AS.seek 1ml @18:36:47.77 delay 1 second @18:36:48.79 AFV.select :holdingCoil @18:36:48.89 delay 1 second @18:36:49.92 AS.reconfigure ASfast @18:36:49.97 AS.seek 0ml @18:37:17.30 delay 3 seconds @18:37:20.33 AFV.select :carrierSolution @18:37:20.43 delay 2 seconds @18:37:22.46 AS.reconfigure ASconfig @18:37:22.51 AS.seek 1ml @18:38:20.31 delay 1 second @18:38:21.33 AFV.select :holdingCoil @18:38:21.43 delay 1 second @18:38:22.46 AS.reconfigure ASfast @18:38:22.51 AS.seek 0ml @18:38:49.85 delay 3 seconds @18:38:52.88 AFV.select :carrierSolution @18:38:52.98 delay 2 seconds @18:38:55.01 AS.reconfigure ASconfig @18:38:55.07 AS.seek 1ml @18:39:52.86 delay 1 second @18:39:53.88 AFV.select :holdingCoil @18:39:53.98 delay 1 second @18:39:55.01 AS.reconfigure ASfast @18:39:55.06 AS.seek 0ml @18:40:22.36 delay 3 seconds @18:40:25.39 AFV.select :carrierSolution @18:40:25.49 delay 2 seconds @18:40:27.52 AS.reconfigure ASconfig @18:40:27.57 AS.seek 1ml @18:41:25.33 delay 1 second @18:41:26.35 AFV.select :holdingCoil @18:41:26.45 delay 1 second @18:41:27.48 AS.reconfigure ASnorm @18:41:27.53 AS.seek 0ml @18:42:18.26 Setting up PCR for NM -- writing /var/log/esp/NM20may1918h450ml.pcr @18:42:18.29 Building PCR Slug from ASV with MM1 mix, PP1 primer @18:42:18.31 Priming with carrier @18:42:18.32 ATV.dial ARV @18:42:18.91 ARV.dial :carrier @18:42:19.26 AS.seek 0.5ml @18:42:44.79 AFV.select :carrierSolution @18:42:44.90 delay 2 seconds @18:42:46.93 AS.reconfigure ASconfig @18:42:46.98 AS.seek 1ml @18:43:16.02 delay 1 second @18:43:17.04 AFV.select :holdingCoil @18:43:17.14 delay 1 second @18:43:18.17 ATV.dial :waste @18:43:19.74 AS.reconfigure ASnorm @18:43:19.79 AS.seek :empty @18:44:10.57 AFV.select :carrierSolution @18:44:10.68 delay 2 seconds @18:44:12.71 AS.reconfigure ASconfig @18:44:12.76 AS.seek 0.5ml @18:44:41.83 delay 1 second @18:44:42.85 AFV.select :holdingCoil @18:44:42.95 Building Slug @18:44:42.96 ATV.dial :ARV @18:44:44.62 delay 2 seconds @18:44:46.65 ARV.dial :air1 @18:44:47.05 delay 2 seconds @18:44:49.14 AS.reconfigure ASslow2 @18:44:49.19 AS.seek 0.506ml @18:44:55.37 delay 2 seconds @18:44:57.39 ARV.dial :MM1 @18:44:57.96 delay 2 seconds @18:44:59.99 Adding MM1 @18:45:00.06 AS.seek 0.524ml @18:45:18.30 delay 25 seconds @18:45:43.33 ARV.dial :PP1 @18:45:43.71 Adding PP1 @18:45:43.72 delay 2 seconds @18:45:45.81 AS.seek 0.53ml @18:45:52.24 delay 10 seconds @18:46:02.27 ARV.dial :air1 @18:46:02.63 adding ARV air @18:46:02.65 delay 2 seconds @18:46:04.79 AS.seek 0.536ml @18:46:11.21 delay 5 seconds @18:46:16.24 ARV.dial :carrier @18:46:16.63 delay 7 seconds @18:46:23.72 AS.seek 0.594ml @18:47:22.89 delay 5 seconds @18:47:27.93 ATV.dial :ASV @18:47:28.32 delay 2 seconds @18:47:30.41 AS.seek 0.6ml @18:47:36.85 delay 4 seconds @18:47:40.88 ATV.dial ARV @18:47:41.51 delay 2 seconds @18:47:43.54 ARV.dial :carrier @18:47:43.55 delay 2 seconds @18:47:45.64 AS.seek 0.65ml @18:48:36.16 delay 4 seconds @18:48:40.19 ATV.dial PCR @18:48:40.77 ASV.dial :waste1 @18:48:40.78 ARV.dial :air1 @18:48:41.18 Positioning Slug by 0.324ml @18:48:41.27 AS.seek 0.326ml @18:54:07.29 delay 5 seconds @18:54:12.32 ATV.dial ARV @18:54:13.06 ASV.dial :PCRlock @18:54:13.45 delay 5 seconds @18:54:18.48 Running NM Profile60C300 thermocycling @18:54:18.49 PCR.program Profile60C300 @18:54:18.60 Profile60C300 should complete in < 1:59:25 @18:54:18.61 PCR.run 2:34:16.25 @20:29:35.54 PCR.off @20:29:36.20 PCR for NM completed -- closed /var/log/esp/NM20may1918h450ml.pcr @20:29:36.27 Cleaning PCR @20:29:36.28 AFV.select :carrierSolution @20:29:36.39 delay 2 seconds @20:29:38.42 AS.reconfigure ASconfig @20:29:38.47 AS.seek 0.5ml @20:29:48.77 delay 1 second @20:29:49.79 AFV.select :holdingCoil @20:29:49.89 ASV.dial :waste1 @20:29:50.27 ATV.dial :air3 @20:29:51.49 AS.reconfigure ASslow1 @20:29:51.54 AS.seek 0.51ml @20:29:53.81 delay 2 seconds @20:29:55.83 ATV.dial :bleach @20:29:56.43 AS.seek 0.81ml @20:30:57.00 delay 3 seconds @20:31:00.02 ATV.dial :air3 @20:31:00.53 delay 3 seconds @20:31:03.62 AS.seek 0.835ml @20:31:08.92 delay 3 seconds @20:31:11.95 ATV.dial :bleach @20:31:13.24 AS.seek 0.82ml @20:31:16.53 delay 3 seconds @20:31:19.56 ARV.dial :waste @20:31:20.14 delay 3 seconds @20:31:23.17 ATV.dial :ARV @20:31:24.03 AS.reconfigure ASconfig @20:31:24.08 AS.seek 0.47ml @20:31:44.50 AFV.select :carrierSolution @20:31:44.61 delay 2 seconds @20:31:46.65 AS.seek 1ml @20:32:17.39 delay 1 second @20:32:18.41 AFV.select :holdingCoil @20:32:18.51 delay 1 second @20:32:19.54 AS.reconfigure ASfast @20:32:19.59 AS.seek 0ml @20:32:46.91 AFV.select :carrierSolution @20:32:47.02 delay 2 seconds @20:32:49.05 AS.reconfigure ASconfig @20:32:49.10 AS.seek 1ml @20:33:46.86 delay 1 second @20:33:47.88 AFV.select :holdingCoil @20:33:47.98 delay 1 second @20:33:49.01 AS.reconfigure ASfast @20:33:49.06 AS.seek 0ml @20:34:16.38 AFV.select :carrierSolution @20:34:16.49 delay 2 seconds @20:34:18.52 AS.reconfigure ASconfig @20:34:18.57 AS.seek 1ml @20:35:16.33 delay 1 second @20:35:17.35 AFV.select :holdingCoil @20:35:17.48 delay 1 second @20:35:18.51 AS.reconfigure ASfast @20:35:18.56 AS.seek 0ml @20:35:45.88 ARV.dial between carrier and air1 @20:35:46.37 ATV.dial :air3 @20:35:47.59 AS.reconfigure ASslow1 @20:35:47.64 AS.seek 0.01ml @20:35:49.94 delay 2 seconds @20:35:51.96 ATV.dial :bleach @20:35:52.56 AS.seek 0.81ml @20:38:33.68 delay 3 seconds @20:38:36.71 ATV.dial :air3 @20:38:37.23 delay 3 seconds @20:38:40.32 AS.seek 0.835ml @20:38:45.62 delay 3 seconds @20:38:48.65 ATV.dial :bleach @20:38:49.25 AS.seek 0.82ml @20:38:52.55 delay 3 seconds @20:38:55.58 ATV.dial PCR @20:38:55.98 AS.reconfigure ASconfig @20:38:56.03 AS.seek :empty @20:39:43.49 PCR.program :bleachProfile @20:39:43.55 PCR program should complete in < 2:45 @20:39:43.56 PCR.run 10:00 @20:42:57.50 PCR.off @20:42:57.53 AFV.select :carrierSolution @20:42:57.64 delay 2 seconds @20:42:59.68 AS.seek 1ml @20:43:57.48 delay 1 second @20:43:58.50 AFV.select :holdingCoil @20:43:58.60 delay 1 second @20:43:59.63 AS.reconfigure ASfast @20:43:59.68 AS.seek 0ml @20:44:27.01 delay 3 seconds @20:44:30.04 AFV.select :carrierSolution @20:44:30.14 delay 2 seconds @20:44:32.17 AS.reconfigure ASconfig @20:44:32.22 AS.seek 1ml @20:45:30.03 delay 1 second @20:45:31.05 AFV.select :holdingCoil @20:45:31.15 delay 1 second @20:45:32.18 AS.reconfigure ASfast @20:45:32.23 AS.seek 0ml @20:45:59.56 delay 3 seconds @20:46:02.59 AFV.select :carrierSolution @20:46:02.69 delay 2 seconds @20:46:04.72 AS.reconfigure ASconfig @20:46:04.78 AS.seek 1ml @20:47:02.54 delay 1 second @20:47:03.56 AFV.select :holdingCoil @20:47:03.66 delay 1 second @20:47:04.69 AS.reconfigure ASfast @20:47:04.74 AS.seek 0ml @20:47:32.08 delay 3 seconds @20:47:35.11 AFV.select :carrierSolution @20:47:35.21 delay 2 seconds @20:47:37.24 AS.reconfigure ASconfig @20:47:37.29 AS.seek 1ml @20:48:35.14 delay 1 second @20:48:36.16 AFV.select :holdingCoil @20:48:36.26 delay 1 second @20:48:37.29 AS.reconfigure ASfast @20:48:37.34 AS.seek 0ml @20:49:04.67 delay 3 seconds @20:49:07.70 AFV.select :carrierSolution @20:49:07.80 delay 2 seconds @20:49:09.83 AS.reconfigure ASconfig @20:49:10.56 AS.seek 1ml @20:50:08.36 delay 1 second @20:50:09.38 AFV.select :holdingCoil @20:50:09.48 delay 1 second @20:50:10.51 AS.reconfigure ASnorm @20:50:10.56 AS.seek 0ml @20:51:01.30 Setting up PCR for IPc -- writing /var/log/esp/IPc20may1920h450ml.pcr @20:51:01.32 Building PCR Slug from ASV with MM3 mix, PP6 primer @20:51:01.33 Priming with carrier @20:51:01.34 ATV.dial ARV @20:51:01.94 ARV.dial :carrier @20:51:02.29 AS.seek 0.5ml @20:51:27.84 AFV.select :carrierSolution @20:51:27.95 delay 2 seconds @20:51:29.98 AS.reconfigure ASconfig @20:51:30.03 AS.seek 1ml @20:51:59.11 delay 1 second @20:52:00.13 AFV.select :holdingCoil @20:52:00.23 delay 1 second @20:52:01.26 ATV.dial :waste @20:52:02.83 AS.reconfigure ASnorm @20:52:02.89 AS.seek :empty @20:52:53.63 AFV.select :carrierSolution @20:52:53.74 delay 2 seconds @20:52:55.77 AS.reconfigure ASconfig @20:52:55.82 AS.seek 0.5ml @20:53:24.91 delay 1 second @20:53:25.93 AFV.select :holdingCoil @20:53:26.03 Building Slug @20:53:26.04 ATV.dial :ARV @20:53:27.69 delay 2 seconds @20:53:29.72 ARV.dial :air1 @20:53:30.11 delay 2 seconds @20:53:32.20 AS.reconfigure ASslow2 @20:53:32.25 AS.seek 0.506ml @20:53:38.67 delay 2 seconds @20:53:40.70 ARV.dial :MM3 @20:53:41.71 delay 2 seconds @20:53:43.74 Adding MM3 @20:53:43.83 AS.seek 0.524ml @20:54:02.28 delay 25 seconds @20:54:27.31 ARV.dial :PP6 @20:54:27.69 Adding PP6 @20:54:27.71 delay 2 seconds @20:54:29.79 AS.seek 0.53ml @20:54:35.97 delay 10 seconds @20:54:46.00 ARV.dial :air1 @20:54:46.78 adding ARV air @20:54:46.80 delay 2 seconds @20:54:48.88 AS.seek 0.536ml @20:54:55.18 delay 5 seconds @20:55:00.21 ARV.dial :carrier @20:55:00.60 delay 7 seconds @20:55:07.69 AS.seek 0.594ml @20:56:06.55 delay 5 seconds @20:56:11.58 ATV.dial :ASV @20:56:11.97 delay 2 seconds @20:56:14.06 AS.seek 0.6ml @20:56:20.41 delay 4 seconds @20:56:24.44 ATV.dial ARV @20:56:24.96 delay 2 seconds @20:56:26.99 ARV.dial :carrier @20:56:27.00 delay 2 seconds @20:56:29.09 AS.seek 0.65ml @20:57:19.50 delay 4 seconds @20:57:23.53 ATV.dial PCR @20:57:24.11 ASV.dial :waste1 @20:57:24.12 ARV.dial :air1 @20:57:24.52 Positioning Slug by 0.324ml @20:57:24.61 AS.seek 0.326ml @21:02:50.66 delay 5 seconds @21:02:55.69 ATV.dial ARV @21:02:56.43 ASV.dial :PCRlock @21:02:56.82 delay 5 seconds @21:03:01.85 Running IPc ProfileIPc thermocycling @21:03:01.87 PCR.program ProfileIPc @21:03:01.97 ProfileIPc should complete in < 1:36:15 @21:03:01.98 PCR.run 2:05:18.75 @22:21:07.16 PCR.off @22:21:07.81 PCR for IPc completed -- closed /var/log/esp/IPc20may1920h450ml.pcr @22:21:07.88 Cleaning PCR @22:21:07.89 AFV.select :carrierSolution @22:21:08.00 delay 2 seconds @22:21:10.09 AS.reconfigure ASconfig @22:21:10.13 AS.seek 0.5ml @22:21:20.44 delay 1 second @22:21:21.46 AFV.select :holdingCoil @22:21:21.56 ASV.dial :waste1 @22:21:21.94 ATV.dial :air3 @22:21:23.17 AS.reconfigure ASslow1 @22:21:23.22 AS.seek 0.51ml @22:21:25.50 delay 2 seconds @22:21:27.52 ATV.dial :bleach @22:21:28.13 AS.seek 0.81ml @22:22:28.71 delay 3 seconds @22:22:31.73 ATV.dial :air3 @22:22:32.27 delay 3 seconds @22:22:35.36 AS.seek 0.835ml @22:22:40.63 delay 3 seconds @22:22:43.66 ATV.dial :bleach @22:22:44.26 AS.seek 0.82ml @22:22:47.55 delay 3 seconds @22:22:50.58 ARV.dial :waste @22:22:51.16 delay 3 seconds @22:22:54.19 ATV.dial :ARV @22:22:55.03 AS.reconfigure ASconfig @22:22:55.08 AS.seek 0.47ml @22:23:15.51 AFV.select :carrierSolution @22:23:15.63 delay 2 seconds @22:23:17.67 AS.seek 1ml @22:23:48.41 delay 1 second @22:23:49.43 AFV.select :holdingCoil @22:23:49.53 delay 1 second @22:23:50.56 AS.reconfigure ASfast @22:23:50.61 AS.seek 0ml @22:24:17.93 AFV.select :carrierSolution @22:24:18.04 delay 2 seconds @22:24:20.07 AS.reconfigure ASconfig @22:24:20.12 AS.seek 1ml @22:25:17.88 delay 1 second @22:25:18.90 AFV.select :holdingCoil @22:25:19.00 delay 1 second @22:25:20.03 AS.reconfigure ASfast @22:25:20.08 AS.seek 0ml @22:25:47.43 AFV.select :carrierSolution @22:25:47.54 delay 2 seconds @22:25:49.57 AS.reconfigure ASconfig @22:25:50.29 AS.seek 1ml @22:26:48.04 delay 1 second @22:26:49.06 AFV.select :holdingCoil @22:26:49.16 delay 1 second @22:26:50.19 AS.reconfigure ASfast @22:26:50.24 AS.seek 0ml @22:27:17.58 ARV.dial between carrier and air1 @22:27:18.07 ATV.dial :air3 @22:27:19.29 AS.reconfigure ASslow1 @22:27:19.34 AS.seek 0.01ml @22:27:21.61 delay 2 seconds @22:27:23.63 ATV.dial :bleach @22:27:24.23 AS.seek 0.81ml @22:30:05.37 delay 3 seconds @22:30:08.40 ATV.dial :air3 @22:30:08.91 delay 3 seconds @22:30:12.00 AS.seek 0.835ml @22:30:17.30 delay 3 seconds @22:30:20.33 ATV.dial :bleach @22:30:20.94 AS.seek 0.82ml @22:30:24.24 delay 3 seconds @22:30:27.27 ATV.dial PCR @22:30:27.65 AS.reconfigure ASconfig @22:30:27.70 AS.seek :empty @22:31:15.16 PCR.program :bleachProfile @22:31:15.22 PCR program should complete in < 2:45 @22:31:15.23 PCR.run 10:00 @22:34:29.17 PCR.off @22:34:29.21 AFV.select :carrierSolution @22:34:29.32 delay 2 seconds @22:34:31.36 AS.seek 1ml @22:35:29.20 delay 1 second @22:35:30.22 AFV.select :holdingCoil @22:35:30.32 delay 1 second @22:35:31.35 AS.reconfigure ASfast @22:35:31.40 AS.seek 0ml @22:35:58.75 delay 3 seconds @22:36:01.78 AFV.select :carrierSolution @22:36:01.88 delay 2 seconds @22:36:03.91 AS.reconfigure ASconfig @22:36:03.97 AS.seek 1ml @22:37:01.78 delay 1 second @22:37:02.80 AFV.select :holdingCoil @22:37:02.90 delay 1 second @22:37:03.93 AS.reconfigure ASfast @22:37:03.98 AS.seek 0ml @22:37:31.34 delay 3 seconds @22:37:34.37 AFV.select :carrierSolution @22:37:34.47 delay 2 seconds @22:37:36.50 AS.reconfigure ASconfig @22:37:36.55 AS.seek 1ml @22:38:34.29 delay 1 second @22:38:35.31 AFV.select :holdingCoil @22:38:35.41 delay 1 second @22:38:36.44 AS.reconfigure ASfast @22:38:36.49 AS.seek 0ml @22:39:03.78 delay 3 seconds @22:39:06.81 AFV.select :carrierSolution @22:39:06.91 delay 2 seconds @22:39:08.94 AS.reconfigure ASconfig @22:39:09.00 AS.seek 1ml @22:40:06.76 delay 1 second @22:40:07.78 AFV.select :holdingCoil @22:40:07.88 delay 1 second @22:40:08.91 AS.reconfigure ASfast @22:40:08.96 AS.seek 0ml @22:40:36.30 delay 3 seconds @22:40:39.33 AFV.select :carrierSolution @22:40:39.43 delay 2 seconds @22:40:41.46 AS.reconfigure ASconfig @22:40:41.51 AS.seek 1ml @22:41:39.33 delay 1 second @22:41:40.35 AFV.select :holdingCoil @22:41:40.45 delay 1 second @22:41:41.48 AS.reconfigure ASnorm @22:41:41.53 AS.seek 0ml @22:42:32.29 Setting up PCR for IPc2 -- writing /var/log/esp/IPc220may1922h450ml.pcr @22:42:32.33 Building PCR Slug from ASV with MM2 mix, PP3 primer @22:42:32.34 Priming with carrier @22:42:32.35 ATV.dial ARV @22:42:32.94 ARV.dial :carrier @22:42:33.29 AS.seek 0.5ml @22:42:58.84 AFV.select :carrierSolution @22:42:58.95 delay 2 seconds @22:43:00.98 AS.reconfigure ASconfig @22:43:01.03 AS.seek 1ml @22:43:30.09 delay 1 second @22:43:31.11 AFV.select :holdingCoil @22:43:31.21 delay 1 second @22:43:32.24 ATV.dial :waste @22:43:33.80 AS.reconfigure ASnorm @22:43:33.85 AS.seek :empty @22:44:24.60 AFV.select :carrierSolution @22:44:24.71 delay 2 seconds @22:44:26.74 AS.reconfigure ASconfig @22:44:26.82 AS.seek 0.5ml @22:44:56.60 delay 1 second @22:44:57.62 AFV.select :holdingCoil @22:44:57.72 Building Slug @22:44:57.73 ATV.dial :ARV @22:44:59.27 delay 2 seconds @22:45:01.30 ARV.dial :air1 @22:45:01.69 delay 2 seconds @22:45:03.78 AS.reconfigure ASslow2 @22:45:03.84 AS.seek 0.506ml @22:45:10.13 delay 2 seconds @22:45:12.15 ARV.dial :MM2 @22:45:13.55 delay 2 seconds @22:45:15.58 Adding MM2 @22:45:15.65 AS.seek 0.524ml @22:45:33.92 delay 25 seconds @22:45:58.95 ARV.dial :PP3 @22:45:59.33 Adding PP3 @22:45:59.35 delay 2 seconds @22:46:01.43 AS.seek 0.53ml @22:46:07.61 delay 10 seconds @22:46:17.64 ARV.dial :air2 @22:46:18.02 adding ARV air @22:46:18.04 delay 2 seconds @22:46:20.12 AS.seek 0.536ml @22:46:26.55 delay 5 seconds @22:46:31.58 ARV.dial :carrier @22:46:32.78 delay 7 seconds @22:46:39.87 AS.seek 0.594ml @22:47:38.39 delay 5 seconds @22:47:43.42 ATV.dial :ASV @22:47:43.80 delay 2 seconds @22:47:45.89 AS.seek 0.6ml @22:47:52.13 delay 4 seconds @22:47:56.16 ATV.dial ARV @22:47:56.68 delay 2 seconds @22:47:58.71 ARV.dial :carrier @22:47:58.72 delay 2 seconds @22:48:00.81 AS.seek 0.65ml @22:48:51.30 delay 4 seconds @22:48:55.33 ATV.dial PCR @22:48:55.91 ASV.dial :waste1 @22:48:55.92 ARV.dial :air1 @22:48:56.31 Positioning Slug by 0.324ml @22:48:56.40 AS.seek 0.326ml @22:54:22.45 delay 5 seconds @22:54:27.48 ATV.dial ARV @22:54:28.21 ASV.dial :PCRlock @22:54:28.60 delay 5 seconds @22:54:33.63 Running IPc2 ProfileIPc thermocycling @22:54:33.64 PCR.program ProfileIPc @22:54:33.75 ProfileIPc should complete in < 1:36:15 @22:54:33.76 PCR.run 2:05:18.75 @00:12:36.61CEST20-May-20 PCR.off @00:12:37.17 PCR for IPc2 completed -- closed /var/log/esp/IPc220may1922h450ml.pcr @00:12:37.18 Full MFB Decontamination @00:12:37.19 Decon extraction column and decon AIV @00:12:37.21 SPE.hold 2:46:40,55C @00:12:37.26 ASV.dial :SPEdelivery @00:12:37.90 delay 20 seconds @00:12:57.93 AFV.select :carrierSolution @00:12:58.03 delay 2 seconds @00:13:00.06 AS.reconfigure ASconfig @00:13:00.11 AS.seek 0.5ml @00:13:10.42 delay 1 second @00:13:11.44 AFV.select :holdingCoil @00:13:11.54 ATV.dial :air1 @00:13:13.35 AS.reconfigure ASslow1 @00:13:13.40 AS.seek 0.52ml @00:13:17.69 ATV.dial :ASV @00:13:19.37 ASV.dial :SPErecovery @00:13:20.02 AS.seek 0.62ml @00:13:40.42 ATV.dial :waste @00:13:42.31 AS.reconfigure ASnorm @00:13:42.36 AS.seek :empty @00:14:14.00 AFV.select :holdingCoil @00:14:14.01 ATV.dial :waste @00:14:14.03 AS.reconfigure ASconfig @00:14:14.07 AS.seek 0ml @00:14:14.35 AFV.select :carrierSolution @00:14:14.47 delay 2 seconds @00:14:16.51 AS.seek 0.5ml @00:14:45.50 delay 1 second @00:14:46.52 AFV.select :holdingCoil @00:14:46.62 ATV.dial :air3 @00:14:47.32 AS.reconfigure ASslow1 @00:14:47.37 AS.seek 0.52ml @00:14:51.69 ATV.dial :air3 @00:14:51.78 AS.seek 0.53ml @00:14:54.05 delay 2 seconds @00:14:56.07 ATV.dial :bleach @00:14:56.54 AS.seek 0.71ml @00:15:33.00 delay 3 seconds @00:15:36.03 ATV.dial :air3 @00:15:36.59 delay 3 seconds @00:15:39.68 AS.seek 0.735ml @00:15:44.98 delay 3 seconds @00:15:48.01 ATV.dial :bleach @00:15:48.63 AS.seek 0.72ml @00:15:51.92 delay 3 seconds @00:15:54.95 ATV.dial ASV @00:15:55.99 ASV.dial :SPErecovery @00:15:56.07 AS.seek 0.365ml @00:17:07.67 ATV.dial :waste @00:17:09.33 AS.reconfigure ASnorm @00:17:09.38 AS.seek :empty @00:17:28.20 ATV.dial :air3 @00:17:28.87 AS.reconfigure ASslow1 @00:17:28.92 AS.seek 0.009ml @00:17:31.20 delay 2 seconds @00:17:33.22 ATV.dial :bleach @00:17:33.70 AS.seek 0.579ml @00:19:28.57 delay 3 seconds @00:19:31.59 ATV.dial :air3 @00:19:32.80 delay 3 seconds @00:19:35.89 AS.seek 0.604ml @00:19:41.29 delay 3 seconds @00:19:44.32 ATV.dial :bleach @00:19:44.93 AS.seek 0.589ml @00:19:48.22 delay 3 seconds @00:19:51.25 ATV.dial :ASV @00:19:52.22 ASV.dial :wasteR @00:19:52.62 AS.seek 0ml @00:21:51.36 delay 1:00 @00:22:51.39 ATV.dial ASV @00:22:51.40 AFV.select :carrierSolution @00:22:51.50 delay 2 seconds @00:22:53.53 AS.reconfigure ASconfig @00:22:53.58 AS.seek 1ml @00:23:51.37 delay 1 second @00:23:52.39 AFV.select :holdingCoil @00:23:52.49 delay 1 second @00:23:53.52 ASV.dial :wasteR @00:23:53.54 AS.reconfigure ASslow1 @00:23:53.58 AS.seek 0ml @00:27:14.96 delay 10 seconds @00:27:24.99 ATV.dial :air3 @00:27:26.42 AS.seek 0.01ml @00:27:28.70 delay 2 seconds @00:27:30.72 ATV.dial :bleach @00:27:31.33 AS.seek 0.58ml @00:29:26.21 delay 3 seconds @00:29:29.24 ATV.dial :air3 @00:29:29.78 delay 3 seconds @00:29:32.87 AS.seek 0.605ml @00:29:38.18 delay 3 seconds @00:29:41.21 ATV.dial :bleach @00:29:41.81 AS.seek 0.59ml @00:29:45.10 delay 3 seconds @00:29:48.13 ATV.dial ASV @00:29:49.12 ASV.dial :SPEdelivery @00:29:49.49 AS.seek 0ml @00:31:48.40 delay 1:00 @00:32:48.43 AFV.select :carrierSolution @00:32:48.53 delay 2 seconds @00:32:50.56 AS.reconfigure ASconfig @00:32:50.61 AS.seek 1ml @00:33:48.36 delay 1 second @00:33:49.38 AFV.select :holdingCoil @00:33:49.48 delay 1 second @00:33:50.51 ATV.dial ASV @00:33:50.52 ASV.dial :SPEdelivery @00:33:50.54 AS.reconfigure ASslow1 @00:33:50.58 AS.seek 0ml @00:37:11.95 delay 10 seconds @00:37:21.98 ATV.dial :air2 @00:37:22.77 AS.reconfigure ASslow0 @00:37:22.81 AS.seek 0.8ml @00:38:43.58 delay 3 seconds @00:38:46.61 ATV.dial :ASV @00:38:47.55 ASV.dial :SPEdelivery @00:38:47.57 AS.reconfigure ASslow1 @00:38:47.62 AS.seek 0.03ml @00:41:22.73 delay 20 seconds @00:41:42.76 ATV.dial :waste,via: :air3 @00:41:44.38 AS.reconfigure ASconfig @00:41:44.43 AS.seek :empty @00:41:46.49 SPE.coast @00:41:46.58 Removing Bleach from Mixing Coil @00:41:46.59 AFV.select :carrierSolution @00:41:46.70 delay 2 seconds @00:41:48.74 AS.seek 0.5ml @00:42:17.84 delay 1 second @00:42:18.86 AFV.select :holdingCoil @00:42:18.96 ATV.dial :ASV @00:42:20.56 ASV.dial :SPErecovery @00:42:21.20 AS.reconfigure ASslow1 @00:42:21.25 AS.seek 0.73ml @00:43:07.77 ASV.dial :wasteD @00:43:08.33 AS.reconfigure ASnorm @00:43:08.39 AS.seek 0ml @00:43:45.49 AFV.select :carrierSolution @00:43:45.60 delay 2 seconds @00:43:47.63 AS.reconfigure ASconfig @00:43:47.68 AS.seek 0.5ml @00:44:16.69 delay 1 second @00:44:17.71 AFV.select :holdingCoil @00:44:17.81 ATV.dial ASV @00:44:17.82 ASV.dial :SPErecovery @00:44:18.46 AS.reconfigure ASslow0 @00:44:18.51 AS.seek 0.3ml @00:44:38.92 ATV.dial :air2 @00:44:39.92 AS.reconfigure ASslow1 @00:44:39.97 AS.seek 0.32ml @00:44:44.32 ATV.dial ASV @00:44:45.32 AS.reconfigure ASslow0 @00:44:45.37 AS.seek 0.57ml @00:45:11.50 ASV.dial :wasteD @00:45:12.08 AS.reconfigure ASnorm @00:45:12.13 AS.seek 0ml @00:45:41.22 AFV.select :carrierSolution @00:45:41.33 delay 2 seconds @00:45:43.36 AS.reconfigure ASconfig @00:45:43.41 AS.seek 0.5ml @00:46:12.44 delay 1 second @00:46:13.46 AFV.select :holdingCoil @00:46:13.56 ATV.dial ASV @00:46:13.57 ASV.dial :SPErecovery @00:46:14.21 AS.reconfigure ASslow0 @00:46:14.26 AS.seek 0.3ml @00:46:34.69 ATV.dial :air2 @00:46:35.70 AS.reconfigure ASslow1 @00:46:35.75 AS.seek 0.32ml @00:46:40.08 ATV.dial ASV @00:46:41.09 AS.reconfigure ASslow0 @00:46:41.14 AS.seek 0.57ml @00:47:06.63 ASV.dial :wasteD @00:47:07.22 AS.reconfigure ASnorm @00:47:07.27 AS.seek 0ml @00:47:36.33 ATV.dial :air2 @00:47:37.26 AS.reconfigure ASslow0 @00:47:37.30 AS.seek 0.3ml @00:48:07.85 ATV.dial :ASV @00:48:08.79 ASV.dial :wasteD @00:48:08.81 AS.reconfigure ASconfig @00:48:08.86 AS.seek 0.05ml @00:48:23.54 ATV.dial :waste @00:48:25.31 AS.seek 0ml @00:48:28.46 ASV.dial :SPEdelivery @00:48:29.43 deconLysateINT @00:48:29.44 ATV.dial :air3 @00:48:30.12 AS.reconfigure ASslow1 @00:48:30.17 AS.seek 0.01ml @00:48:32.44 delay 2 seconds @00:48:34.46 ATV.dial :bleach @00:48:34.92 AS.seek 0.96ml @00:51:46.24 delay 3 seconds @00:51:49.27 ATV.dial :air3 @00:51:49.80 delay 3 seconds @00:51:52.89 AS.seek 0.985ml @00:51:58.19 delay 3 seconds @00:52:01.22 ATV.dial :bleach @00:52:01.83 AS.seek 0.97ml @00:52:05.10 delay 3 seconds @00:52:08.13 ATV.dial AIV @00:52:09.32 AIV.dial :coilToMFB @00:52:09.34 AWV.select :waste @00:52:09.35 AFV.select :carrierSolution @00:52:09.46 delay 2 seconds @00:52:11.49 AS.reconfigure ASconfig @00:52:11.54 AS.seek 1ml @00:52:13.57 delay 1 second @00:52:14.59 AFV.select :holdingCoil @00:52:14.69 delay 1 second @00:52:15.72 AS.reconfigure ASslow0 @00:52:15.77 AS.seek :empty @00:53:56.73 AFV.select :carrierSolution @00:53:56.84 delay 2 seconds @00:53:58.87 AS.reconfigure ASconfig @00:53:58.92 AS.seek 1ml @00:54:56.71 delay 1 second @00:54:57.73 AFV.select :holdingCoil @00:54:57.84 delay 1 second @00:54:58.87 AS.reconfigure ASslow0 @00:54:58.92 AS.seek :empty @00:56:39.92 AFV.select :carrierSolution @00:56:40.03 delay 2 seconds @00:56:42.06 AS.reconfigure ASconfig @00:56:42.11 AS.seek 1ml @00:57:39.92 delay 1 second @00:57:40.94 AFV.select :holdingCoil @00:57:41.04 delay 1 second @00:57:42.07 AS.reconfigure ASslow0 @00:57:42.12 AS.seek :empty @00:59:23.06 ATV.dial :air2 @00:59:23.72 AS.reconfigure ASslow1 @00:59:23.77 AS.seek 0.009ml @00:59:26.10 AS.reconfigure ASslow0 @00:59:26.16 AS.seek 1ml @01:01:06.09 ATV.dial AIV @01:01:06.83 AS.seek 0.02ml @01:02:45.73 ATV.dial :waste @01:02:47.29 AS.seek 0ml @01:02:49.61 AWV.select :air @01:02:49.78 Cleaning PCR @01:02:49.79 AFV.select :carrierSolution @01:02:50.53 delay 2 seconds @01:02:52.59 AS.reconfigure ASconfig @01:02:52.64 AS.seek 0.5ml @01:03:21.67 delay 1 second @01:03:22.69 AFV.select :holdingCoil @01:03:22.79 ASV.dial :waste1 @01:03:23.15 ATV.dial :air3 @01:03:23.80 AS.reconfigure ASslow1 @01:03:23.85 AS.seek 0.51ml @01:03:26.14 delay 2 seconds @01:03:28.16 ATV.dial :bleach @01:03:28.61 AS.seek 0.81ml @01:04:29.20 delay 3 seconds @01:04:32.23 ATV.dial :air3 @01:04:32.76 delay 3 seconds @01:04:35.85 AS.seek 0.835ml @01:04:41.12 delay 3 seconds @01:04:44.15 ATV.dial :bleach @01:04:44.76 AS.seek 0.82ml @01:04:48.08 delay 3 seconds @01:04:51.11 ARV.dial :waste @01:04:51.70 delay 3 seconds @01:04:54.73 ATV.dial :ARV @01:04:55.58 AS.reconfigure ASconfig @01:04:55.63 AS.seek 0.47ml @01:05:16.06 AFV.select :carrierSolution @01:05:16.17 delay 2 seconds @01:05:18.21 AS.seek 1ml @01:05:49.00 delay 1 second @01:05:50.02 AFV.select :holdingCoil @01:05:50.12 delay 1 second @01:05:51.15 AS.reconfigure ASfast @01:05:51.20 AS.seek 0ml @01:06:18.56 AFV.select :carrierSolution @01:06:18.67 delay 2 seconds @01:06:20.70 AS.reconfigure ASconfig @01:06:20.75 AS.seek 1ml @01:07:18.53 delay 1 second @01:07:19.55 AFV.select :holdingCoil @01:07:19.65 delay 1 second @01:07:20.68 AS.reconfigure ASfast @01:07:20.73 AS.seek 0ml @01:07:48.06 AFV.select :carrierSolution @01:07:48.17 delay 2 seconds @01:07:50.20 AS.reconfigure ASconfig @01:07:50.25 AS.seek 1ml @01:08:48.06 delay 1 second @01:08:49.08 AFV.select :holdingCoil @01:08:49.18 delay 1 second @01:08:50.21 AS.reconfigure ASfast @01:08:50.26 AS.seek 0ml @01:09:17.61 ARV.dial between carrier and air1 @01:09:18.10 ATV.dial :air3 @01:09:19.34 AS.reconfigure ASslow1 @01:09:19.39 AS.seek 0.01ml @01:09:21.69 delay 2 seconds @01:09:23.71 ATV.dial :bleach @01:09:24.32 AS.seek 0.81ml @01:12:05.52 delay 3 seconds @01:12:08.55 ATV.dial :air3 @01:12:09.08 delay 3 seconds @01:12:12.17 AS.seek 0.835ml @01:12:17.44 delay 3 seconds @01:12:20.47 ATV.dial :bleach @01:12:21.07 AS.seek 0.82ml @01:12:24.36 delay 3 seconds @01:12:27.38 ATV.dial PCR @01:12:27.77 AS.reconfigure ASconfig @01:12:27.82 AS.seek :empty @01:13:15.28 PCR.program :bleachProfile @01:13:15.34 PCR program should complete in < 2:45 @01:13:15.35 PCR.run 10:00 @01:16:44.44 PCR.off @01:16:44.48 AFV.select :carrierSolution @01:16:44.59 delay 2 seconds @01:16:46.63 AS.seek 1ml @01:17:44.48 delay 1 second @01:17:45.50 AFV.select :holdingCoil @01:17:45.60 delay 1 second @01:17:46.63 AS.reconfigure ASfast @01:17:46.68 AS.seek 0ml @01:18:14.02 delay 3 seconds @01:18:17.05 AFV.select :carrierSolution @01:18:17.67 delay 2 seconds @01:18:19.78 AS.reconfigure ASconfig @01:18:19.83 AS.seek 1ml @01:19:17.57 delay 1 second @01:19:18.59 AFV.select :holdingCoil @01:19:18.69 delay 1 second @01:19:19.72 AS.reconfigure ASfast @01:19:19.77 AS.seek 0ml @01:19:47.10 delay 3 seconds @01:19:50.13 AFV.select :carrierSolution @01:19:50.23 delay 2 seconds @01:19:52.26 AS.reconfigure ASconfig @01:19:52.31 AS.seek 1ml @01:20:50.05 delay 1 second @01:20:51.07 AFV.select :holdingCoil @01:20:51.17 delay 1 second @01:20:52.20 AS.reconfigure ASfast @01:20:52.25 AS.seek 0ml @01:21:19.56 delay 3 seconds @01:21:22.59 AFV.select :carrierSolution @01:21:22.69 delay 2 seconds @01:21:24.72 AS.reconfigure ASconfig @01:21:24.78 AS.seek 1ml @01:22:22.59 delay 1 second @01:22:23.61 AFV.select :holdingCoil @01:22:23.71 delay 1 second @01:22:24.74 AS.reconfigure ASfast @01:22:24.79 AS.seek 0ml @01:22:52.13 delay 3 seconds @01:22:55.16 AFV.select :carrierSolution @01:22:55.26 delay 2 seconds @01:22:57.29 AS.reconfigure ASconfig @01:22:57.35 AS.seek 1ml @01:23:55.14 delay 1 second @01:23:56.16 AFV.select :holdingCoil @01:23:56.26 delay 1 second @01:23:57.29 AS.reconfigure ASnorm @01:23:57.34 AS.seek 0ml @01:24:48.07 AFV.select :carrierSolution @01:24:48.18 delay 2 seconds @01:24:50.21 AS.reconfigure ASconfig @01:24:50.26 AS.seek 1ml @01:25:48.03 delay 1 second @01:25:49.05 AFV.select :holdingCoil @01:25:49.15 delay 1 second @01:25:50.18 ATV.dial :waste @01:25:51.35 AS.reconfigure ASnorm @01:25:51.39 AS.seek 0.5ml @01:26:16.95 ATV.dial :waste2 @01:26:18.18 AS.seek 0ml @01:26:43.71 AS.coast @01:26:43.76 SS.coast @01:26:43.79 CC.coast @01:26:43.83 CS.coast @01:26:43.87 PC.coast @01:26:43.91 PS.coast @01:26:43.95 Forearm.coast @01:26:44.00 Elbow.coast @01:26:44.39 Gate.power :core,:OFF @01:26:44.40 Gate.power :raw,:OFF @01:26:44.41 Shutdown @01:26:44.43 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @10:00:00.10
Powering core bus @10:00:05.24 Gate.power :raw,:ON @10:00:05.90 SPE.coast @10:00:05.96 SPE.configure SPEconfig @10:00:06.05 AS.configure ASconfig @10:00:06.15 SS.configure SSconfig @10:00:06.25 CC.configure CCconfig @10:00:06.42 CS.configure CSconfig @10:00:06.52 PC.configure PCconfig @10:00:06.62 PS.configure PSconfig @10:00:07.24 Forearm.configure LooseForearm @10:00:07.42 Elbow.configure LooseElbow @10:00:07.54 SC.configure SCloose @10:00:07.64 SE.configure SEconfig @10:00:07.69 CH.coast @10:00:07.74 CH.configure CHconfig @10:00:07.78 PH.coast @10:00:07.84 PH.configure PHconfig @10:00:07.89 PCR.off @10:00:07.92 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:07.95 PCR.caltemp= 2.8814,0.0372,0 @10:00:07.98 All dwarf controllers running firmware version 3.75 @10:00:08.09 Valve.reset @10:00:08.26 PCR.off @10:00:08.30 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:08.33 PCR.caltemp= 2.8814,0.0372,0 @10:00:08.49 Intake.select :close @10:00:08.53 Exhaust.select :close @10:00:08.57 CTV.dial :air @10:00:08.67 PSV.dial :air @10:00:08.71 CSV.dial :waste1 @10:00:08.88 SSV.dial :spit,avoiding: :exhaust @10:00:08.90
Forearm.reconfigure LooseForearm @10:00:09.03 Forearm.home.seek :retract @10:00:09.24 PS.reconfigure PSconfig @10:00:09.29 PS.home.seek 0ml @10:00:09.84 Hand.close @10:00:09.88
Elbow.jog 1500 @10:00:10.01 CS.reconfigure CSfast @10:00:10.04 SS.reconfigure SSconfig @10:00:10.15 CS.home.seek 0ml @10:00:10.17 SS.home.seek 0ml @10:00:10.66
Elbow.reconfigure LooseElbow @10:00:10.71 Elbow.home.seek :clear @10:00:14.18 SE.reconfigure SEconfig @10:00:14.25 SE.home.seek :down @10:00:14.97 SC.reconfigure SCsloppy @10:00:15.02 SC.home.seek :initial @10:00:17.26 Forearm.seek :retract @10:00:17.33 Elbow.seek :hammer @10:00:18.32 PS.coast @10:00:18.64
Forearm.reconfigure TightForearm @10:00:18.68 Forearm.seek 5 + 350 counts @10:00:19.73 Forearm.seek :retract @10:00:20.98 PC.reconfigure PCconfig @10:00:20.99
CC.reconfigure CCconfig @10:00:21.10 CC.home.seek :open @10:00:21.11 PC.home.seek :open @10:00:24.65 GoodNews.email "At 10:00:24 began in tube 2: wcrphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:129\n{1 => 21, 2 => 1}\nCan@10:00:24, 8.2C, 45% humidity, 14.3psia, 13.539V, 0.809A, 0.809A avg, 10.95W",Subject: "Started 20invasivedeployfinal phase wcrphase" @10:00:24.68
Phase [wcrphase] @10:00:24.72CEST20-May-20 ===> STARTED WCRSphase (phase #7) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @10:00:24.73 Starting initialPurge @10:00:24.74 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:00:24.82 Hand.open @10:00:25.51 Elbow.reconfigure TightElbow @10:00:25.56 Elbow.seek FlushPuck::Garage @10:00:27.59 Elbow.reconfigure LooseElbow @10:00:27.69 Forearm.reconfigure LooseForearm @10:00:27.74 Forearm.seek :garage @10:00:28.75 Hand.close @10:00:29.71 Forearm.seek :retract @10:00:30.78 Elbow.reconfigure TightElbow @10:00:30.90 Elbow.seek CC @10:00:34.04 Elbow.reconfigure LooseElbow @10:00:34.30 Forearm.reconfigure TightForearm @10:00:34.42 Forearm.seek CC @10:00:36.19 CC.seek :closed @10:00:40.62 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.03 @10:00:40.84 Hand.open @10:00:41.70 Forearm.reconfigure LooseForearm @10:00:41.78 Forearm.seek :retract @10:00:42.80 Flushing the Collection Valve::Manifold @10:00:43.09 CSV.dial CSR @10:00:44.63 Pulling 5ml of CSR.flush @10:00:44.65 CSR.select :flush @10:00:44.84 CS.reconfigure CSconfig @10:00:44.94 CS.seek 5ml @10:00:58.77 delay 5 seconds @10:01:03.80 CSR.select :air @10:01:04.10 CS.reconfigure CSfast @10:01:04.20 CS.seek 7.5ml @10:01:11.16 CSV.dial :waste @10:01:12.59 CS.reconfigure CSconfig @10:01:12.68 CS.seek 0ml @10:01:32.84 Purging external Intake @10:01:32.98 CSV.dial :flush @10:01:33.46 CS.reconfigure CSfast @10:01:33.51 CS.seek 10ml @10:01:57.30 delay 5 seconds @10:02:02.33 CSV.dial CBV @10:02:02.37
CBV.dial CSV @10:02:03.28 CTV.dial :intake @10:02:04.11 Intake.select :open @10:02:04.23 CS.seek 0ml @10:02:29.58 Intake.select :close @10:02:29.69 CTV.dial :air @10:02:30.49 Flushing upper manifold @10:02:30.62 CSV.dial :flush @10:02:31.39 CS.seek 5ml @10:02:43.74 delay 5 seconds @10:02:48.77 CSV.dial :air @10:02:49.89 CS.seek 7ml @10:02:55.49 CSV.dial CBV @10:02:55.51
CBV.dial CSV @10:02:56.60 CTV.dial :air @10:02:56.62 CS.seek 0ml @10:03:13.52 delay 7 seconds @10:03:20.56 CS.seek 3ml @10:03:28.36 CSV.dial :waste @10:03:28.84 CS.seek 0ml @10:03:36.73 CSV.dial :flush @10:03:37.20 CS.seek 3ml @10:03:45.11 delay 5 seconds @10:03:50.14 CSV.dial :air @10:03:50.67 CS.seek 5.001ml @10:03:56.33 CSV.dial :air @10:03:56.35 CS.seek 0ml @10:04:08.68 delay 7 seconds @10:04:15.72 CS.seek 3ml @10:04:23.49 CSV.dial :waste @10:04:24.27 CS.seek 0ml @10:04:32.08 Garaging FlushPuck in Collection Clamp @10:04:32.16 Elbow.reconfigure TightElbow @10:04:32.21 Elbow.seek CC @10:04:32.46 Elbow.reconfigure LooseElbow @10:04:32.57 Forearm.seek CC @10:04:33.59 Hand.close @10:04:34.26 CC.seek :open @10:04:38.65 Forearm.seek :retract @10:04:39.64 Elbow.reconfigure TightElbow @10:04:39.69 Elbow.seek :garage @10:04:42.98 Elbow.reconfigure LooseElbow @10:04:43.08 Forearm.reconfigure TightForearm @10:04:43.13 Forearm.seek :garage @10:04:44.29 Hand.open @10:04:44.96 Forearm.reconfigure LooseForearm @10:04:45.00 Forearm.seek :retract @10:04:46.04 Starting stand-alone bac WCR @10:04:46.05 bac wcr sampling at most 1500ml @10:04:46.08 Whole Cell Archive sampling 1500ml @10:04:46.09 BACfans.email "Started stand-alone bac WCR in tube 2 at 10:04:46CEST20-May-20\n{1 => 21, 2 => 1}\nCan@10:00:24, 8.2C, 45% humidity, 14.3psia, 13.539V, 0.809A, 0.809A avg, 10.95W",Subject: "bac wcr sampling at most 1500ml" @10:04:46.14 Loading wcr puck from tube 2 into Collection Clamp @10:04:46.46 SC.reconfigure SCloose @10:04:46.73 Elbow.reconfigure TightElbow @10:04:46.79 Elbow.seek 2 @10:04:48.92 Forearm.reconfigure TightForearm @10:04:48.97 Forearm.seek 2 + 700 counts @10:04:50.00 SE.reconfigure SEpick @10:04:50.11 SE.seek beamBreak @10:04:53.19 SE.configure SEconfig @10:04:54.94 Elbow.seek 2 @10:04:55.41 Forearm.seek 2 + 300 counts @10:04:55.67 Forearm.reconfigure LooseForearm @10:04:55.77 Hand.close @10:04:56.48 SE.seek :down @10:04:56.97 Forearm.seek :retract @10:04:57.98 Elbow.seek CC @10:04:59.40 SE.coast @10:04:59.92 Elbow.reconfigure LooseElbow @10:05:00.16 Forearm.reconfigure TightForearm @10:05:00.31 Forearm.seek CC @10:05:02.02 CC.seek :closed @10:05:06.48 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.02 @10:05:06.51 Hand.open @10:05:06.71 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[64:27->20] @10:05:07.34 Forearm.reconfigure LooseForearm @10:05:07.44 Forearm.seek :retract @10:05:08.56 SC.coast @10:05:08.64 collectSample 1500ml w/bubble point of 55psi @10:05:08.68 Priming 7ml @10:05:08.70 Intake.select :close @10:05:08.72 Exhaust.select :close @10:05:08.75 SS.hold @10:05:09.24 Calibrating pressure and temperature sensors @10:05:09.28 CTV.dial :air @10:05:09.31 SSV.dial :spit,avoiding: :exhaust @10:05:09.38 SS.reconfigure SSfast @10:05:09.49 SS.seek 5ml @10:05:12.09 SSV.dial CBV @10:05:12.16 CBV.dial SSV @10:05:13.65 delay 3.8 seconds @10:05:17.74 SSV.dial :spit @10:05:19.20 SS.seek 0ml @10:05:21.89 SSV.dial CBV @10:05:23.34 CTV.dial :intake @10:05:24.24 SSV.dial CBV @10:05:24.29 CBV.dial SSV @10:05:24.35 Allowing 40s for 7.0ml of remaining 7.0ml @10:05:24.38 Intake.select :open @10:05:26.69 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:05:26.73 SS.reconfigure SSinhale.with maxSpeed: 150 @10:05:26.83 SS.seek 7ml @10:05:32.64 Stroke completed -- waiting for pressure to equalize ... @10:05:46.22 Intake.select :close @10:05:46.46 SSV.dial :exhaust @10:05:47.32 Exhaust.select :open @10:05:47.50 SS.reconfigure SSexhale @10:05:47.60 SS.seek 0ml @10:05:51.47 Exhaust.select :close @10:05:51.63 Sampling 1500.0ml @10:05:51.66 Intake.select :close @10:05:51.68 Exhaust.select :close @10:05:51.71 SS.reconfigure SSconfig @10:05:51.81 SS.hold @10:05:52.25 CTV.dial :intake @10:05:52.29 SSV.dial CBV @10:05:52.36 CBV.dial SSV @10:05:53.25 Allowing 130s for 25.0ml of remaining 1500.0ml @10:05:53.28 Intake.select :open @10:05:55.58 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:05:55.62 SS.reconfigure SSinhale.with maxSpeed: 150 @10:05:55.71 SS.seek 25ml @10:05:59.48 Vacuum too high -- waiting up to 40s @10:06:02.05 Intake @30.8psia, vacuum from 20.0 to 24.0psi @10:06:02.07 SS.reconfigure SSinhale.with maxSpeed: 135 @10:06:02.12 SS.seek 25ml @10:06:05.15 Vacuum too high -- waiting up to 40s @10:06:08.22 Intake @30.8psia, vacuum from 20.0 to 24.0psi @10:06:08.24 SS.reconfigure SSinhale.with maxSpeed: 122 @10:06:08.29 SS.seek 25ml @10:06:11.32 Vacuum too high -- waiting up to 40s @10:06:14.75 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:06:14.77 SS.reconfigure SSinhale.with maxSpeed: 110 @10:06:14.82 SS.seek 25ml @10:06:17.91 Vacuum too high -- waiting up to 40s @10:06:20.55 SS.reconfigure SSinhale.with maxSpeed: 99 @10:06:20.60 SS.seek 25ml @10:06:23.60 Vacuum too high -- waiting up to 40s @10:06:26.19 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:06:26.21 SS.reconfigure SSinhale.with maxSpeed: 89 @10:06:26.26 SS.seek 25ml @10:06:28.86 Vacuum too high -- waiting up to 40s @10:06:31.43 Intake @30.8psia, vacuum from 20.0 to 24.0psi @10:06:31.45 SS.reconfigure SSinhale.with maxSpeed: 80 @10:06:31.50 SS.seek 25ml @10:06:34.37 Vacuum too high -- waiting up to 40s @10:06:36.94 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:06:36.96 SS.reconfigure SSinhale.with maxSpeed: 72 @10:06:37.01 SS.seek 25ml @10:06:40.11 Vacuum too high -- waiting up to 40s @10:06:42.55 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:06:42.57 SS.reconfigure SSinhale.with maxSpeed: 65 @10:06:42.61 SS.seek 25ml @10:06:43.55 Stroke completed -- waiting for pressure to equalize ... @10:06:47.40 Intake.select :close @10:06:47.56 SSV.dial :exhaust @10:06:48.38 Exhaust.select :open @10:06:48.50 SS.reconfigure SSexhale @10:06:48.55 SS.seek 0ml @10:06:57.63 Exhaust.select :close @10:06:57.75 SSV.dial CBV @10:06:57.77 CBV.dial SSV @10:06:58.60 Allowing 130s for 25.0ml of remaining 1475.0ml @10:06:58.61 Intake.select :open @10:07:00.79 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:07:00.81 SS.reconfigure SSinhale.with maxSpeed: 65 @10:07:00.86 SS.seek 25ml @10:07:06.00 Vacuum too high -- waiting up to 40s @10:07:07.84 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:07:07.86 SS.reconfigure SSinhale.with maxSpeed: 59 @10:07:07.91 SS.seek 25ml @10:07:12.30 Vacuum too high -- waiting up to 40s @10:07:14.63 SS.reconfigure SSinhale.with maxSpeed: 53 @10:07:14.68 SS.seek 25ml @10:07:20.82 Vacuum too high -- waiting up to 40s @10:07:23.16 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:07:23.18 SS.reconfigure SSinhale.with maxSpeed: 48 @10:07:23.22 SS.seek 25ml @10:07:29.63 Vacuum too high -- waiting up to 40s @10:07:32.06 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:07:32.08 SS.reconfigure SSinhale.with maxSpeed: 43 @10:07:32.13 SS.seek 25ml @10:07:39.83 Vacuum too high -- waiting up to 40s @10:07:42.20 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:07:42.22 SS.reconfigure SSinhale.with maxSpeed: 39 @10:07:42.27 SS.seek 25ml @10:07:51.44 Vacuum too high -- waiting up to 40s @10:07:54.09 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:07:54.11 SS.reconfigure SSinhale.with maxSpeed: 35 @10:07:54.16 SS.seek 25ml @10:07:55.03 Stroke completed -- waiting for pressure to equalize ... @10:07:59.46 Intake.select :close @10:07:59.62 SSV.dial :exhaust @10:08:00.45 Exhaust.select :open @10:08:00.57 SS.reconfigure SSexhale @10:08:00.62 SS.seek 0ml @10:08:09.57 Exhaust.select :close @10:08:09.69 SSV.dial CBV @10:08:09.71 CBV.dial SSV @10:08:10.52 Allowing 130s for 25.0ml of remaining 1450.0ml @10:08:10.53 Intake.select :open @10:08:12.71 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:08:12.73 SS.reconfigure SSinhale.with maxSpeed: 35 @10:08:12.78 SS.seek 25ml @10:08:25.36 Vacuum too high -- waiting up to 40s @10:08:27.68 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:08:27.70 SS.reconfigure SSinhale.with maxSpeed: 32 @10:08:27.75 SS.seek 25ml @10:08:38.47 Vacuum too high -- waiting up to 40s @10:08:41.66 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:08:41.68 SS.reconfigure SSinhale.with maxSpeed: 29 @10:08:41.73 SS.seek 25ml @10:08:53.89 Vacuum too high -- waiting up to 40s @10:08:57.21 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:08:57.23 SS.reconfigure SSinhale.with maxSpeed: 26 @10:08:57.28 SS.seek 25ml @10:09:12.70 Vacuum too high -- waiting up to 40s @10:09:16.28 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:09:16.30 SS.reconfigure SSinhale.with maxSpeed: 23 @10:09:16.35 SS.seek 25ml @10:09:28.27 Stroke completed -- waiting for pressure to equalize ... @10:09:38.30 Intake.select :close @10:09:39.08 SSV.dial :exhaust @10:09:39.90 Exhaust.select :open @10:09:40.02 SS.reconfigure SSexhale @10:09:40.07 SS.seek 0ml @10:09:49.15 Exhaust.select :close @10:09:49.27 SSV.dial CBV @10:09:49.29 CBV.dial SSV @10:09:50.12 Allowing 130s for 25.0ml of remaining 1425.0ml @10:09:50.13 Intake.select :open @10:09:52.31 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:09:52.33 SS.reconfigure SSinhale.with maxSpeed: 23 @10:09:52.39 SS.seek 25ml @10:10:14.61 Vacuum too high -- waiting up to 40s @10:10:17.47 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:10:17.49 SS.reconfigure SSinhale.with maxSpeed: 21 @10:10:17.54 SS.seek 25ml @10:10:40.16 Vacuum too high -- waiting up to 40s @10:10:43.99 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:10:44.01 SS.reconfigure SSinhale.with maxSpeed: 19 @10:10:44.06 SS.seek 25ml @10:11:21.51 Vacuum too high -- waiting up to 40s @10:11:26.71 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:11:26.73 SS.reconfigure SSinhale.with maxSpeed: 17 @10:11:26.78 SS.seek 25ml @10:11:31.13 Stroke completed -- waiting for pressure to equalize ... @10:11:40.63 Intake.select :close @10:11:40.79 SSV.dial :exhaust @10:11:41.62 Exhaust.select :open @10:11:41.74 SS.reconfigure SSexhale @10:11:41.80 SS.seek 0ml @10:11:50.68 Exhaust.select :close @10:11:50.80 SSV.dial CBV @10:11:50.82 CBV.dial SSV @10:11:51.67 Allowing 130s for 25.0ml of remaining 1400.0ml @10:11:51.68 Intake.select :open @10:11:53.85 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:11:53.87 SS.reconfigure SSinhale.with maxSpeed: 17 @10:11:53.92 SS.seek 25ml @10:13:20.20 Vacuum too high -- waiting up to 40s @10:13:24.02 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:13:24.04 SS.reconfigure SSinhale.with maxSpeed: 15 @10:13:24.09 SS.seek 25ml @10:13:43.86 Stroke completed -- waiting for pressure to equalize ... @10:13:55.14 Intake.select :close @10:13:55.30 SSV.dial :exhaust @10:13:56.12 Exhaust.select :open @10:13:56.24 SS.reconfigure SSexhale @10:13:56.29 SS.seek 0ml @10:14:05.15 Exhaust.select :close @10:14:05.27 SSV.dial CBV @10:14:05.29 CBV.dial SSV @10:14:06.14 Allowing 130s for 25.0ml of remaining 1375.0ml @10:14:06.15 Intake.select :open @10:14:08.34 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:14:08.36 SS.reconfigure SSinhale.with maxSpeed: 15 @10:14:08.41 SS.seek 25ml @10:16:05.30 Stroke completed -- waiting for pressure to equalize ... @10:16:16.16 SS.reconfigure SSconfig @10:16:16.21 SS.hold @10:16:16.34 Intake.select :close @10:16:16.50 ShallowSampler::Clogged in WCR -- progress too slow after 150.0ml @10:16:16.52 SSV.dial :exhaust @10:16:17.34 Exhaust.select :open @10:16:17.46 SS.reconfigure SSexhale @10:16:17.51 SS.seek 0ml @10:16:26.61 Exhaust.select :close @10:16:26.72 Sampled 150.0ml @10:16:26.74 Drying puck before fixing for 4:00 @10:16:26.75 CBV.dial :waste2b @10:16:27.57 delay 1:00 @10:17:27.61 CSV.dial :air @10:17:28.50 CS.seek 10ml @10:17:52.19 Pumping wcr puck from tube 2 up to 55psi... @10:17:52.20 CBV.dial SSV @10:17:53.58 CBV.dial between CSV and waste2b @10:17:54.62 CSV.dial between air and CTV @10:17:54.97 CS.seek 7.154ml @10:18:02.52 CTV.dial CSV,avoiding: :air @10:18:02.55 CSV.dial CTV @10:18:03.37 CS.reconfigure CSpressurize @10:18:03.43 CS.seek 0ml @10:18:21.62 CBV.dial :waste2b @10:18:21.98 CS.reconfigure CSconfig @10:18:22.03 CS.seek :empty @10:18:23.51 delay 1:00 @10:19:23.54 CTV.dial :air,avoiding: :intake @10:19:24.95 Fix #1 of 2 (with rnal) for 2:00 @10:19:25.54 CSV.dial CSR @10:19:26.08 Pulling 2ml of CSR.rnal @10:19:26.10 CSR.select :rnal @10:19:26.22 CS.seek 2ml @10:19:32.45 delay 5 seconds @10:19:37.48 CSR.select :air @10:19:37.61 CS.reconfigure CSfast @10:19:37.66 CS.seek 10ml @10:19:56.70 Pumping wcr puck from tube 2 up to 55psi... @10:19:56.72 CBV.dial SSV @10:19:58.08 CBV.dial between CSV and waste2b @10:19:59.13 CSV.dial between air and CTV @10:19:59.77 CS.seek 7.219ml @10:20:07.14 CTV.dial CSV,avoiding: :air @10:20:07.16 CSV.dial CTV @10:20:08.64 CS.reconfigure CSpressurize @10:20:08.69 CS.seek 0ml @10:20:27.00 delay 2:00 @10:22:27.04 CBV.dial :waste2b @10:22:27.39 CS.reconfigure CSconfig @10:22:27.44 CS.seek :empty @10:22:29.01 delay 1:00 @10:23:29.04 CTV.dial :air,avoiding: :intake @10:23:30.44 Fix #2 of 2 (with rnal) for 2:00 @10:23:30.46 CSV.dial CSR @10:23:30.98 Pulling 2ml of CSR.rnal @10:23:30.99 CSR.select :rnal @10:23:31.12 CS.seek 2ml @10:23:37.30 delay 5 seconds @10:23:42.33 CSR.select :air @10:23:42.45 CS.reconfigure CSfast @10:23:42.50 CS.seek 10ml @10:24:01.48 Pumping wcr puck from tube 2 up to 55psi... @10:24:01.49 CBV.dial SSV @10:24:02.86 CBV.dial between CSV and waste2b @10:24:03.90 CSV.dial between air and CTV @10:24:04.54 CS.seek 7.219ml @10:24:11.93 CTV.dial CSV,avoiding: :air @10:24:11.95 CSV.dial CTV @10:24:13.41 CS.reconfigure CSpressurize @10:24:13.46 CS.seek 0ml @10:24:31.66 delay 2:00 @10:26:31.69 CBV.dial :waste2b @10:26:32.05 CS.reconfigure CSconfig @10:26:32.10 CS.seek :empty @10:26:34.05 delay 1:00 @10:27:34.08 CTV.dial :air,avoiding: :intake @10:27:35.56 Storing wcr puck in Collection Clamp to tube 1 @10:27:35.75 SC.reconfigure SCtight @10:27:35.80 SC.seek 1 @10:27:37.93 SC.reconfigure SCloose @10:27:38.15 Elbow.reconfigure TightElbow @10:27:38.20 Elbow.seek 1 @10:27:40.66 Forearm.reconfigure TightForearm @10:27:40.73 Forearm.seek 1 + 700 counts @10:27:41.78 SE.reconfigure SEpick @10:27:41.86 SE.seek beamBreak @10:27:43.03 SE.configure SEconfig @10:27:43.20 Elbow.reconfigure LimpElbow @10:27:43.25 Forearm.reconfigure LooseForearm @10:27:43.30 Forearm.seek 1 @10:27:43.58 Elbow.hold @10:27:43.66 Elbow.reconfigure TightElbow @10:27:43.77 SE.seek 20.11 Pucks below Top Plate @10:27:44.34 Forearm.force -20 @10:27:44.39 Forearm.seek 1 + 346 counts @10:27:44.71 Forearm.reconfigure TightForearm @10:27:44.76 Forearm.seek 1 + 700 counts @10:27:45.07 SE.seek 21.11 Pucks below Top Plate @10:27:45.77 Forearm.reconfigure LooseForearm @10:27:45.82 Forearm.seek :retract @10:27:46.74 Elbow.seek CC @10:27:48.46 Elbow.reconfigure LooseElbow @10:27:48.57 Forearm.seek CC @10:27:49.61 Hand.close @10:27:50.19 CC.seek :open @10:27:54.68 Forearm.seek :retract @10:27:55.69 Elbow.reconfigure TightElbow @10:27:55.74 Elbow.seek 1 @10:27:57.70 Elbow.reconfigure LimpElbow @10:27:57.76 Forearm.seek 1 @10:27:58.76 Elbow.hold @10:27:58.84 Elbow.reconfigure TightElbow @10:27:58.89 Hand.open @10:27:59.68 Forearm.force -20 @10:27:59.73 Forearm.seek 1 + 346 counts @10:28:00.05 Forearm.reconfigure TightForearm @10:28:00.10 Forearm.seek 1 + 700 counts @10:28:00.51 SE.seek :down @10:28:01.26 SE.coast @10:28:01.66 Forearm.reconfigure LooseForearm @10:28:01.71 Forearm.seek :retract @10:28:02.68 SC.coast @10:28:02.72 Starting finalPurge @10:28:02.74 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:28:02.81 Hand.open @10:28:03.19 Elbow.seek FlushPuck::Garage @10:28:05.81 Elbow.reconfigure LooseElbow @10:28:05.92 Forearm.seek :garage @10:28:06.91 Hand.close @10:28:07.69 Forearm.seek :retract @10:28:08.68 Elbow.reconfigure TightElbow @10:28:08.73 Elbow.seek CC @10:28:11.61 Elbow.reconfigure LooseElbow @10:28:11.72 Forearm.reconfigure TightForearm @10:28:11.77 Forearm.seek CC @10:28:13.09 CC.seek :closed @10:28:17.34 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.92 @10:28:17.43 Hand.open @10:28:18.13 Forearm.reconfigure LooseForearm @10:28:18.18 Forearm.seek :retract @10:28:19.18 Flushing the Collection Valve::Manifold @10:28:19.31 CSV.dial CSR @10:28:19.83 Pulling 5ml of CSR.flush @10:28:19.84 CSR.select :flush @10:28:19.97 CS.seek 5ml @10:28:33.83 delay 5 seconds @10:28:38.86 CSR.select :air @10:28:39.03 CS.reconfigure CSfast @10:28:39.08 CS.seek 7.5ml @10:28:45.86 CSV.dial :waste @10:28:47.21 CS.reconfigure CSconfig @10:28:47.26 CS.seek 0ml @10:29:07.55 Flushing lower manifold's waste1 port @10:29:07.68 CSV.dial :flush @10:29:08.15 CS.reconfigure CSfast @10:29:08.20 CS.seek 5ml @10:29:20.52 delay 5 seconds @10:29:25.55 CSV.dial :air @10:29:26.09 CS.seek 7ml @10:29:31.68 CSV.dial CTV @10:29:31.70
CTV.dial CSV @10:29:33.19 CBV.dial :waste1 @10:29:34.00 CS.seek 0ml @10:29:50.83 Flushing lower manifold's waste2b port @10:29:50.96 CSV.dial :flush @10:29:51.75 CS.seek 5ml @10:30:04.02 delay 5 seconds @10:30:09.05 CSV.dial :air @10:30:09.61 CS.seek 7ml @10:30:15.24 CSV.dial CTV @10:30:15.26
CTV.dial CSV @10:30:15.74 CBV.dial :waste2b @10:30:16.55 CS.seek 0ml @10:30:33.49 Flushing upper manifold @10:30:33.62 CSV.dial :flush @10:30:34.41 CS.seek 5ml @10:30:46.71 delay 5 seconds @10:30:51.74 CSV.dial :air @10:30:52.30 CS.seek 7ml @10:30:57.96 CSV.dial CBV @10:30:58.58
CBV.dial CSV @10:30:59.73 CTV.dial :air @10:31:01.13 CS.seek 0ml @10:31:17.96 delay 7 seconds @10:31:25.00 CS.seek 3ml @10:31:32.84 CSV.dial :waste @10:31:33.33 CS.seek 0ml @10:31:41.46 CSV.dial :flush @10:31:41.94 CS.seek 3ml @10:31:49.74 delay 5 seconds @10:31:54.77 CSV.dial :air @10:31:55.31 CS.seek 5ml @10:32:00.93 CSV.dial :air @10:32:00.95 CS.seek 0ml @10:32:13.24 delay 7 seconds @10:32:20.28 CS.seek 3ml @10:32:28.18 CSV.dial :waste @10:32:28.96 CS.seek 0ml @10:32:36.96 Disinfecting external Intake with mfbkill @10:32:37.10 CSV.dial CSR @10:32:38.51 Pulling 7.999ml of CSR.mfbkill @10:32:38.52 CSR.select :mfbkill @10:32:38.64 CS.seek 8ml @10:32:57.78 delay 5 seconds @10:33:02.81 CSR.select :air @10:33:02.94 CS.seek 10ml @10:33:08.46 CSV.dial CBV @10:33:08.50
CBV.dial CSV @10:33:09.57 CTV.dial :intake @10:33:10.35 Intake.select :open @10:33:10.47 CS.seek 2ml @10:33:30.71 Intake.select :close @10:33:30.82 CTV.dial :air @10:33:31.62 CS.reconfigure CSconfig @10:33:31.67 CS.seek 0ml @10:33:37.84 Flushing lower manifold's waste1 port @10:33:37.85 CSV.dial CSR @10:33:38.98 Pulling 4ml of CSR.mfbkill @10:33:38.99 CSR.select :mfbkill @10:33:39.11 CS.seek 4ml @10:33:50.34 delay 5 seconds @10:33:55.37 CSR.select :air @10:33:55.50 CS.reconfigure CSfast @10:33:55.54 CS.seek 10ml @10:34:10.03 CSV.dial CTV @10:34:10.06
CTV.dial CSV @10:34:11.53 CBV.dial :waste1 @10:34:12.04 CS.seek 0ml @10:34:35.50 Flushing lower manifold's waste2b port @10:34:35.51 CSV.dial CSR @10:34:36.06 Pulling 4ml of CSR.mfbkill @10:34:36.07 CSR.select :mfbkill @10:34:36.20 CS.reconfigure CSconfig @10:34:36.25 CS.seek 4ml @10:34:47.56 delay 5 seconds @10:34:52.59 CSR.select :air @10:34:52.72 CS.reconfigure CSfast @10:34:52.76 CS.seek 10ml @10:35:07.25 CSV.dial CTV @10:35:07.29
CTV.dial CSV @10:35:07.76 CBV.dial :waste2b @10:35:08.58 CS.seek 0ml @10:35:32.25 Killing upper manifold @10:35:32.26 CSV.dial CSR @10:35:32.79 Pulling 4ml of CSR.mfbkill @10:35:32.80 CSR.select :mfbkill @10:35:32.92 CS.reconfigure CSconfig @10:35:32.96 CS.seek 4ml @10:35:44.15 delay 5 seconds @10:35:49.18 CSR.select :air @10:35:49.31 CS.reconfigure CSfast @10:35:49.36 CS.seek 10ml @10:36:03.84 CSV.dial CBV @10:36:03.86
CBV.dial CSV @10:36:04.98 CTV.dial :air @10:36:06.37 CS.seek 0ml @10:36:29.81 delay 7 seconds @10:36:36.85 CS.seek 3ml @10:36:45.19 CSV.dial :waste @10:36:45.79 CS.seek 0ml @10:36:53.59 Flushing upper manifold @10:36:53.73 CSV.dial :flush @10:36:54.21 CS.seek 5ml @10:37:06.50 delay 5 seconds @10:37:11.53 CSV.dial :air @10:37:12.07 CS.seek 7ml @10:37:17.62 CSV.dial CBV @10:37:17.65
CBV.dial CSV @10:37:18.73 CTV.dial :air @10:37:18.75 CS.seek 0ml @10:37:35.66 delay 7 seconds @10:37:42.70 CS.seek 3ml @10:37:50.47 CSV.dial :waste @10:37:50.95 CS.seek 0ml @10:37:59.08 CSV.dial :flush @10:37:59.57 CS.seek 3ml @10:38:07.50 delay 5 seconds @10:38:12.53 CSV.dial :air @10:38:13.07 CS.seek 5ml @10:38:18.69 CSV.dial :air @10:38:18.71 CS.seek 0ml @10:38:31.03 delay 7 seconds @10:38:38.07 CS.seek 3ml @10:38:46.06 CSV.dial :waste @10:38:46.84 CS.seek 0ml @10:38:54.63 Flushing lower manifold's waste1 port @10:38:54.77 CSV.dial :flush @10:38:55.26 CS.seek 5ml @10:39:07.60 delay 5 seconds @10:39:12.63 CSV.dial :air @10:39:13.29 CS.seek 7.001ml @10:39:18.91 CSV.dial CTV @10:39:18.95
CTV.dial CSV @10:39:20.41 CBV.dial :waste1 @10:39:20.92 CS.seek 0ml @10:39:37.85 Flushing lower manifold's waste2b port @10:39:37.98 CSV.dial :flush @10:39:38.76 CS.seek 5ml @10:39:51.03 delay 5 seconds @10:39:56.06 CSV.dial :air @10:39:56.62 CS.seek 7ml @10:40:02.19 CSV.dial CTV @10:40:02.23
CTV.dial CSV @10:40:02.69 CBV.dial :waste2b @10:40:03.53 CS.seek 0ml @10:40:20.25 Disinfecting external Intake with kill @10:40:20.38 CSV.dial CSR @10:40:20.91 Pulling 8ml of CSR.kill @10:40:20.92 CSR.select :kill @10:40:21.04 CS.seek 8ml @10:40:40.04 delay 5 seconds @10:40:45.07 CSR.select :air @10:40:45.20 CS.seek 10ml @10:40:50.75 CSV.dial CBV @10:40:50.77
CBV.dial CSV @10:40:51.89 CTV.dial :intake @10:40:52.68 Intake.select :open @10:40:52.80 CS.seek 2ml @10:41:13.10 Intake.select :close @10:41:13.21 CTV.dial :air @10:41:14.00 CS.reconfigure CSconfig @10:41:14.05 CS.seek 0ml @10:41:20.22 CSV.dial CSR @10:41:21.35 Pulling 5ml of CSR.flush @10:41:21.36 CSR.select :flush @10:41:21.48 CS.seek 5ml @10:41:35.35 delay 5 seconds @10:41:40.38 CSR.select :air @10:41:41.09 CS.seek 10ml @10:41:54.82 CSV.dial CBV @10:41:55.91 CS.seek 0ml @10:42:22.44 Garaging FlushPuck in Collection Clamp @10:42:22.52 Elbow.reconfigure TightElbow @10:42:22.57 Elbow.seek CC @10:42:22.81 Elbow.reconfigure LooseElbow @10:42:22.92 Forearm.seek CC @10:42:23.95 Hand.close @10:42:24.58 CC.seek :open @10:42:28.95 Forearm.seek :retract @10:42:29.97 Elbow.reconfigure TightElbow @10:42:30.02 Elbow.seek :garage @10:42:33.26 Elbow.reconfigure LooseElbow @10:42:33.37 Forearm.reconfigure TightForearm @10:42:33.42 Forearm.seek :garage @10:42:34.56 Hand.open @10:42:35.27 Forearm.reconfigure LooseForearm @10:42:35.32 Forearm.seek :retract @10:42:36.34 Starting initialPurge @10:42:36.35 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:42:36.43 Hand.open @10:42:36.77 Elbow.reconfigure TightElbow @10:42:36.81 Elbow.seek FlushPuck::Garage @10:42:37.04 Elbow.reconfigure LooseElbow @10:42:37.15 Forearm.seek :garage @10:42:38.16 Hand.close @10:42:38.96 Forearm.seek :retract @10:42:39.96 Elbow.reconfigure TightElbow @10:42:40.01 Elbow.seek CC @10:42:42.95 Elbow.reconfigure LooseElbow @10:42:43.05 Forearm.reconfigure TightForearm @10:42:43.10 Forearm.seek CC @10:42:44.43 CC.seek :closed @10:42:48.69 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.93 @10:42:48.76 Hand.open @10:42:49.40 Forearm.reconfigure LooseForearm @10:42:49.44 Forearm.seek :retract @10:42:50.45 Flushing the Collection Valve::Manifold @10:42:50.59 CSV.dial CSR @10:42:51.72 Pulling 5ml of CSR.flush @10:42:51.73 CSR.select :flush @10:42:51.85 CS.seek 5ml @10:43:05.69 delay 5 seconds @10:43:10.72 CSR.select :air @10:43:10.89 CS.reconfigure CSfast @10:43:10.94 CS.seek 7.5ml @10:43:17.98 CSV.dial :waste @10:43:19.33 CS.reconfigure CSconfig @10:43:19.38 CS.seek 0ml @10:43:39.54 Purging external Intake @10:43:39.67 CSV.dial :flush @10:43:40.15 CS.reconfigure CSfast @10:43:40.20 CS.seek 10ml @10:44:03.60 delay 5 seconds @10:44:08.62 CSV.dial CBV @10:44:08.67
CBV.dial CSV @10:44:09.43 CTV.dial :intake @10:44:10.82 Intake.select :open @10:44:10.94 CS.seek 0ml @10:44:36.01 Intake.select :close @10:44:36.12 CTV.dial :air @10:44:36.92 Flushing upper manifold @10:44:37.05 CSV.dial :flush @10:44:37.85 CS.seek 5ml @10:44:50.17 delay 5 seconds @10:44:55.20 CSV.dial :air @10:44:55.74 CS.seek 7ml @10:45:01.26 CSV.dial CBV @10:45:01.30
CBV.dial CSV @10:45:02.37 CTV.dial :air @10:45:02.39 CS.seek 0ml @10:45:19.13 delay 7 seconds @10:45:26.17 CS.seek 3ml @10:45:33.92 CSV.dial :waste @10:45:34.41 CS.seek 0ml @10:45:42.29 CSV.dial :flush @10:45:42.75 CS.seek 3ml @10:45:50.60 delay 5 seconds @10:45:55.63 CSV.dial :air @10:45:56.16 CS.seek 5.001ml @10:46:01.64 CSV.dial :air @10:46:01.66 CS.seek 0ml @10:46:13.92 delay 7 seconds @10:46:20.96 CS.seek 3ml @10:46:28.79 CSV.dial :waste @10:46:29.57 CS.seek 0ml @10:46:37.35 Garaging FlushPuck in Collection Clamp @10:46:37.43 Elbow.reconfigure TightElbow @10:46:37.48 Elbow.seek CC @10:46:37.74 Elbow.reconfigure LooseElbow @10:46:37.85 Forearm.seek CC @10:46:38.85 Hand.close @10:46:39.46 CC.seek :open @10:46:43.83 Forearm.seek :retract @10:46:44.84 Elbow.reconfigure TightElbow @10:46:44.89 Elbow.seek :garage @10:46:47.93 Elbow.reconfigure LooseElbow @10:46:48.04 Forearm.reconfigure TightForearm @10:46:48.08 Forearm.seek :garage @10:46:49.25 Hand.open @10:46:49.91 Forearm.reconfigure LooseForearm @10:46:49.95 Forearm.seek :retract @10:46:50.97 Completed [wcrphase] begun 10:00:24 @10:46:50.99 Preparing to sleep until 16:00 20-may-20 @10:46:51.06 SS.coast @10:46:51.09
Asleep til 16:00 20-may-20 @10:46:51.16 CC.coast @10:46:51.22
Continuing at 16:00 @10:46:51.24 CS.coast @10:46:51.35 PC.coast @10:46:51.38 GoodNews.email "Then, in tube 3: wcrphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:133\n{1 => 22, 2 => 0}\nCan@10:46:51, 9.1C, 44% humidity, 14.3psia, 13.584V, 0.449A, 0.449A avg, 6.10W",Subject: "Asleep until 16:00" @10:46:51.46 Forearm.coast @10:46:51.50 Elbow.coast @10:46:51.89 Gate.power :core,:OFF @10:46:51.91 Gate.power :raw,:OFF @10:46:51.92 Shutdown @16:00:00.05
Powering core bus @16:00:05.04 Gate.power :raw,:ON @16:00:05.70 SPE.coast @16:00:05.77 SPE.configure SPEconfig @16:00:05.86 AS.configure ASconfig @16:00:05.97 SS.configure SSconfig @16:00:06.07 CC.configure CCconfig @16:00:06.16 CS.configure CSconfig @16:00:06.26 PC.configure PCconfig @16:00:06.36 PS.configure PSconfig @16:00:06.48 Forearm.configure LooseForearm @16:00:06.58 Elbow.configure LooseElbow @16:00:06.70 SC.configure SCloose @16:00:06.79 SE.configure SEconfig @16:00:06.84 CH.coast @16:00:06.90 CH.configure CHconfig @16:00:06.94 PH.coast @16:00:07.00 PH.configure PHconfig @16:00:07.04 PCR.off @16:00:07.07 PCR.pidtemp= 50,0.1,0,300,25,25 @16:00:07.10 PCR.caltemp= 2.8814,0.0372,0 @16:00:07.13 All dwarf controllers running firmware version 3.75 @16:00:07.24 Valve.reset @16:00:07.41 PCR.off @16:00:07.45 PCR.pidtemp= 50,0.1,0,300,25,25 @16:00:07.48 PCR.caltemp= 2.8814,0.0372,0 @16:00:07.75 PSV.dial :air @16:00:07.78 Intake.select :close @16:00:07.86 Exhaust.select :close @16:00:07.89 CTV.dial :air @16:00:07.97
Forearm.reconfigure LooseForearm @16:00:08.02 Forearm.home.seek :retract @16:00:08.09 CSV.dial :waste1 @16:00:08.12 PS.reconfigure PSconfig @16:00:08.23 PS.home.seek 0ml @16:00:08.24 SSV.dial :spit,avoiding: :exhaust @16:00:08.78 Hand.close @16:00:08.81
Elbow.jog 1500 @16:00:09.15 CS.reconfigure CSfast @16:00:09.18 SS.reconfigure SSconfig @16:00:09.29 CS.home.seek 0ml @16:00:09.33 SS.home.seek 0ml @16:00:09.48 Slide::Error in rdyProcessing -- Processing Syringe overCurrent at 0.305ml @16:00:09.50 Waiting to Resume from Checkpoint "Processing Syringe overCurrent at 0.305ml" @16:00:09.51 BadNews.email "Processing Syringe overCurrent at 0.305ml",Subject: "Slide::Error in rdyProcessing" @16:00:09.58
Elbow.reconfigure LooseElbow @16:00:09.62 Elbow.home.seek :clear @16:00:13.04 SE.reconfigure SEconfig @16:00:13.11 SE.home.seek :down @16:00:13.74 SC.reconfigure SCsloppy @16:00:13.82 SC.home.seek :initial @16:00:16.19 Forearm.seek :retract @16:00:16.37 Elbow.seek :hammer @16:00:17.69 Forearm.reconfigure TightForearm @16:00:17.79 Forearm.seek 5 + 350 counts @16:00:18.99 Forearm.seek :retract @16:00:21.80 CC.reconfigure CCconfig @16:00:21.86 PC.reconfigure PCconfig @16:00:22.10 PC.home.seek :open @16:00:22.15
CC.home.seek :open @16:38:29.54 Session Begun @16:38:41.13 -> showlog 50 @16:40:41.76 -> MainThread.recover @16:40:41.78 Thread::CannotResume in bkha -- MAIN is not suspended @16:42:42.98 -> showlog 100 @16:44:37.73 -> ESP.ready @16:44:37.75 NoMethodError in bkha -- undefined method `ready' for ESP:Module @16:51:49.34 Session Begun @16:51:53.56 -> showlog 10 @16:51:57.40 -> ESP.ready! @16:52:58.20 -> MainThread.resume @16:52:58.22 Thread::CannotResume in bkha10 -- MAIN is not suspended @17:00:38.95 Session Begun @17:00:45.09 -> showlog 10 @17:01:04.28 -> PS.home.to :home @17:01:04.32 PS.reconfigure PSconfig @17:01:04.46 PS.home.seek :home @17:01:28.93 -> Thread[:rdyProcessing].recover @17:01:28.96 Recovering Thread after Processing Syringe overCurrent at 0.305ml at 16:00:09 @17:01:29.06 PS.seek 0ml @17:01:29.46 Slide::Error in rdyProcessing -- Processing Syringe overCurrent at 0.345ml @17:01:29.48 Waiting to Resume from Checkpoint "Processing Syringe overCurrent at 0.345ml" @17:01:29.54 BadNews.email "Processing Syringe overCurrent at 0.345ml",Subject: "Slide::Error in rdyProcessing" @17:04:09.67 -> PSV @17:06:26.03 -> PSV.legend @17:07:47.62 Session Ended @17:10:41.25 -> ESP.ready? @17:11:17.25 -> PSV.waste @17:11:17.28 NoMethodError in bkha11 -- undefined method `waste' for PSV:Shaft @17:11:25.89 -> PSV.waste1 @17:11:25.91 PSV.dial :waste1 @17:11:31.33 -> PS.empty @17:11:31.37 PS.seek :empty @17:11:31.78 Slide::Error in bkha11 -- Processing Syringe overCurrent at 0.345ml @17:13:33.78 -> PSV.air @17:13:33.80 PSV.dial :air @17:13:45.30 -> PS.to 0.400 @17:13:45.39 PS.seek 0.4ml @17:13:58.10 -> PS.to 0.500 @17:13:58.13 PS.seek 0.5ml @17:14:27.22 -> PS.to 0.6 @17:14:27.25 PS.seek 0.6ml @17:14:34.90 -> PS.to 0.9 @17:14:34.95 PS.seek 0.9ml @17:14:47.71 -> PSV.waste1 @17:14:47.73 PSV.dial :waste1 @17:15:02.74 -> PS.to 0.3 @17:15:02.77 PS.seek 0.3ml @17:15:08.94 Slide::Error in bkha11 -- Processing Syringe overCurrent at 0.341ml @17:15:19.04 -> PSV @17:15:30.90 -> PSV.air @17:15:30.92 PSV.dial :air @17:16:27.20 -> PSV @17:16:31.66 -> PS.fill @17:16:31.77 Session Ended @17:16:31.82 PS.seek 2.5ml @17:18:47.21 -> PSV.waste1 @17:18:47.23 PSV.dial :waste1 @17:19:05.77 -> PS.to 0.5 @17:19:05.80 PS.seek 0.5ml @17:19:46.10 -> PS.to 0.4 @17:19:46.13 PS.seek 0.4ml @17:19:53.78 -> PS.to 0.3 @17:19:53.81 PS.seek 0.3ml @17:19:54.88 Slide::Error in bkha11 -- Processing Syringe overCurrent at 0.341ml @17:20:21.62 -> PSV.air @17:20:21.64 PSV.dial :air @17:20:27.62 -> PS.fill @17:20:27.69 PS.seek 2.5ml @17:23:37.57 Session Begun @17:23:38.69 -> espclient preston @17:23:38.74 NameError in preston -- undefined local variable or method `preston' for # @17:23:53.52 Session Begun @17:23:57.71 -> showlog -100 @17:24:01.89 -> PSV.waste2 @17:24:01.92 PSV.dial :waste2 @17:24:18.78 -> PS.to 0.5 @17:24:18.82 PS.seek 0.5ml @17:24:53.34 -> PS.to 0.4 @17:24:53.38 PS.seek 0.4ml @17:24:59.74 -> PS.to 0.3 @17:24:59.78 PS.seek 0.3ml @17:25:00.92 Slide::Error in bkha11 -- Processing Syringe overCurrent at 0.341ml @17:25:19.24 -> PS.status @17:25:34.05 -> PS.status @17:26:10.13 -> Log.comment "Maybe try to ready the PS agai manually" @17:26:10.15 Maybe try to ready the PS agai manually @17:26:41.36 -> PS.home:to :home @17:26:41.39 SyntaxError in bkha11 -- compile error bkha11:32: syntax error, unexpected ':', expecting $end PS.home:to :home ^ @17:26:51.61 -> PS.home.to :home @17:26:51.64 PS.reconfigure PSconfig @17:26:51.78 PS.home.seek :home @17:27:14.47 -> PS @17:27:18.31 -> PS.status @17:27:24.23 -> PS @17:28:05.19 -> #is this how you write? @17:28:18.32 -> #good trick @17:28:39.05 -> PS.status @17:29:04.62 -> # should we move to air and see if we can move to 1ml? @17:29:13.34 -> PSV.air @17:29:13.36 PSV.dial :air @17:29:22.62 -> PS.to 1 @17:29:22.66 PS.seek 1ml @17:29:45.67 -> #it seems to do alright, turning valves and moving up @17:30:35.58 -> # head back to 0.5 @17:30:36.54 -> PSV.waste1 @17:30:36.56 PSV.dial :waste1 @17:30:48.06 -> PS.to 0.5 @17:30:48.10 PS.seek 0.5ml @17:31:10.09 -> #ps. to 0.4? @17:31:55.20 -> # go back to 1ml then head to empty. Lets try some momenteum @17:32:29.19 -> PS.to 1 @17:32:29.23 PS.seek 1ml @17:32:38.47 -> PS.empty @17:32:38.50 PS.seek :empty @17:32:54.47 -> #wub wub @17:33:12.71 -> #ps.homo.to :home? @17:33:33.06 -> PSV @17:33:42.39 -> #ok... now try the resume with PSV at air @17:33:52.08 -> PSV.air @17:33:52.10 PSV.dial :air @17:34:16.39 -> # the thread recover ? @17:34:32.72 -> #yes rdyProcessing @17:34:52.23 -> Thread[:rdyProcessing].recover @17:34:52.26 Recovering Thread after Processing Syringe overCurrent at 0.345ml at 17:01:29 @17:34:52.36 PS.seek 0ml @17:34:52.79 PS.coast @17:34:53.51
Phase [wcrphase] @17:34:53.52 GoodNews.email "At 17:34:53 began in tube 3: wcrphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:133\n{1 => 22, 2 => 0}\nCan@17:34:53, 8.6C, 45% humidity, 14.4psia, 13.589V, 0.506A, 0.506A avg, 6.87W",Subject: "Started 20invasivedeployfinal phase wcrphase" @17:34:53.66CEST20-May-20
===> STARTED WCRSphase (phase #8) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @17:34:53.69 Starting initialPurge @17:34:53.76 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @17:34:53.92 Hand.open @17:34:54.79 Elbow.reconfigure TightElbow @17:34:54.91 Elbow.seek FlushPuck::Garage @17:34:56.90 Elbow.reconfigure LooseElbow @17:34:57.11 Forearm.reconfigure LooseForearm @17:34:57.21 Forearm.seek :garage @17:34:58.32 Hand.close @17:34:59.16 Forearm.seek :retract @17:35:00.32 Elbow.reconfigure TightElbow @17:35:00.52 Elbow.seek CC @17:35:03.73 Elbow.reconfigure LooseElbow @17:35:04.04 Forearm.reconfigure TightForearm @17:35:04.29 Forearm.seek CC @17:35:06.11 CC.seek :closed @17:35:10.74 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.04 @17:35:11.11 Hand.open @17:35:12.03 Forearm.reconfigure LooseForearm @17:35:12.25 Forearm.seek :retract @17:35:13.45 Flushing the Collection Valve::Manifold @17:35:13.86 CSV.dial CSR @17:35:15.57 Pulling 5ml of CSR.flush @17:35:15.59 CSR.select :flush @17:35:15.92 CS.reconfigure CSconfig @17:35:16.10 CS.seek 5ml @17:35:24.17 -> #funny quote there Brian! @17:35:29.93
delay 5 seconds @17:35:34.96 CSR.select :air @17:35:35.34 CS.reconfigure CSfast @17:35:35.54 CS.seek 7.5ml @17:35:42.42 CSV.dial :waste @17:35:43.43 -> #ok fingers crossed and good luck! @17:35:43.96
CS.reconfigure CSconfig @17:35:44.15 CS.seek 0ml @17:35:45.47 -> exit @17:35:45.50 Session Ended @17:36:04.43
Purging external Intake @17:36:04.60 CSV.dial :flush @17:36:05.11 CS.reconfigure CSfast @17:36:05.21 CS.seek 10ml @17:36:28.90 delay 5 seconds @17:36:33.93 CSV.dial CBV @17:36:33.97
CBV.dial CSV @17:36:34.83 CTV.dial :intake @17:36:35.65 Intake.select :open @17:36:35.79 CS.seek 0ml @17:37:00.99 Intake.select :close @17:37:01.12 CTV.dial :air @17:37:01.96 Flushing upper manifold @17:37:02.13 CSV.dial :flush @17:37:03.55 CS.seek 5ml @17:37:15.96 delay 5 seconds @17:37:20.99 CSV.dial :air @17:37:21.56 CS.seek 7ml @17:37:27.24 CSV.dial CBV @17:37:27.28
CBV.dial CSV @17:37:28.40 CTV.dial :air @17:37:28.45 CS.seek 0ml @17:37:45.34 delay 7 seconds @17:37:52.38 CS.seek 3ml @17:38:00.34 CSV.dial :waste @17:38:00.86 CS.seek 0ml @17:38:08.89 CSV.dial :flush @17:38:09.41 CS.seek 3ml @17:38:17.27 delay 5 seconds @17:38:22.30 CSV.dial :air @17:38:22.88 CS.seek 5ml @17:38:28.49 CSV.dial :air @17:38:28.52 CS.seek 0ml @17:38:40.96 delay 7 seconds @17:38:48.00 CS.seek 3ml @17:38:55.96 CSV.dial :waste @17:38:56.77 CS.seek 0ml @17:39:04.71 Garaging FlushPuck in Collection Clamp @17:39:04.84 Elbow.reconfigure TightElbow @17:39:04.92 Elbow.seek CC @17:39:05.22 Elbow.reconfigure LooseElbow @17:39:05.36 Forearm.seek CC @17:39:06.39 Hand.close @17:39:07.00 CC.seek :open @17:39:11.40 Forearm.seek :retract @17:39:12.45 Elbow.reconfigure TightElbow @17:39:12.54 Elbow.seek :garage @17:39:15.83 Elbow.reconfigure LooseElbow @17:39:15.98 Forearm.reconfigure TightForearm @17:39:16.06 Forearm.seek :garage @17:39:17.22 Hand.open @17:39:17.95 Forearm.reconfigure LooseForearm @17:39:18.04 Forearm.seek :retract @17:39:19.14 Starting stand-alone bac WCR @17:39:19.15 bac wcr sampling at most 1500ml @17:39:19.24 Whole Cell Archive sampling 1500ml @17:39:19.27 BACfans.email "Started stand-alone bac WCR in tube 3 at 17:39:19CEST20-May-20\n{1 => 22, 2 => 0}\nCan@17:35:03, 8.7C, 45% humidity, 14.3psia, 13.554V, 0.638A, 0.638A avg, 8.65W",Subject: "bac wcr sampling at most 1500ml" @17:39:19.32 Loading wcr puck from tube 3 into Collection Clamp @17:39:19.71 SC.reconfigure SCtight @17:39:19.81 SC.seek 3 @17:39:22.03 SC.reconfigure SCloose @17:39:22.33 Elbow.reconfigure TightElbow @17:39:22.45 Elbow.seek 3 @17:39:24.76 Forearm.reconfigure TightForearm @17:39:24.85 Forearm.seek 3 + 700 counts @17:39:27.07 SE.reconfigure SEpick @17:39:27.29 SE.seek beamBreak @17:39:28.46 SE.configure SEconfig @17:39:28.95 Elbow.seek 3 @17:39:29.51 Forearm.seek 3 + 300 counts @17:39:29.94 Forearm.reconfigure LooseForearm @17:39:30.11 Hand.close @17:39:30.82 SE.seek :down @17:39:31.40 Forearm.seek :retract @17:39:31.86 SE.coast @17:39:32.50 Elbow.seek CC @17:39:34.43 Elbow.reconfigure LooseElbow @17:39:34.78 Forearm.reconfigure TightForearm @17:39:34.99 Forearm.seek CC @17:39:36.78 CC.seek :closed @17:39:41.35 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.09 @17:39:41.39 Hand.open @17:39:42.33 Forearm.reconfigure LooseForearm @17:39:42.49 Forearm.seek :retract @17:39:43.62 SC.coast @17:39:43.77 collectSample 1500ml w/bubble point of 55psi @17:39:43.80 Priming 7ml @17:39:43.88 Intake.select :close @17:39:43.96 Exhaust.select :close @17:39:44.04 SS.hold @17:39:44.66 Calibrating pressure and temperature sensors @17:39:44.71 CTV.dial :air @17:39:44.78 SSV.dial :spit,avoiding: :exhaust @17:39:44.91 SS.reconfigure SSfast @17:39:45.12 SS.seek 5ml @17:39:48.19 SSV.dial CBV @17:39:48.34 CBV.dial SSV @17:39:49.75 delay 3.8 seconds @17:39:53.84 SSV.dial :spit @17:39:55.42 SS.seek 0ml @17:39:58.03 SSV.dial CBV @17:39:59.51 CTV.dial :intake @17:40:00.46 SSV.dial CBV @17:40:00.56 CBV.dial SSV @17:40:00.66 Allowing 40s for 7.0ml of remaining 7.0ml @17:40:00.74 Intake.select :open @17:40:03.13 Intake @30.9psia, vacuum from 20.0 to 24.0psi @17:40:03.18 SS.reconfigure SSinhale.with maxSpeed: 150 @17:40:03.39 SS.seek 7ml @17:40:07.38 Vacuum too high -- waiting up to 40s @17:40:12.37 Intake @31.1psia, vacuum from 20.0 to 24.0psi @17:40:12.44 SS.reconfigure SSinhale.with maxSpeed: 135 @17:40:12.66 SS.seek 7ml @17:40:15.81 Stroke completed -- waiting for pressure to equalize ... @17:40:20.50 Session Ended @17:40:24.56 Intake.select :close @17:40:24.84 SSV.dial :exhaust @17:40:25.75 Exhaust.select :open @17:40:25.97 SS.reconfigure SSexhale @17:40:26.12 SS.seek 0ml @17:40:30.01 Exhaust.select :close @17:40:30.24 Sampling 1500.0ml @17:40:30.27 Intake.select :close @17:40:30.31 Exhaust.select :close @17:40:30.39 SS.reconfigure SSconfig @17:40:30.56 SS.hold @17:40:31.03 CTV.dial :intake @17:40:31.07 SSV.dial CBV @17:40:31.16 CBV.dial SSV @17:40:32.09 Allowing 130s for 25.0ml of remaining 1500.0ml @17:40:32.15 Intake.select :open @17:40:34.40 Intake @31.1psia, vacuum from 20.0 to 24.0psi @17:40:34.42 SS.reconfigure SSinhale.with maxSpeed: 135 @17:40:34.52 SS.seek 25ml @17:40:38.00 Vacuum too high -- waiting up to 40s @17:40:41.57 Intake @31.2psia, vacuum from 20.0 to 24.0psi @17:40:41.59 SS.reconfigure SSinhale.with maxSpeed: 122 @17:40:41.69 SS.seek 25ml @17:40:44.31 Vacuum too high -- waiting up to 40s @17:40:47.75 Intake @31.1psia, vacuum from 20.0 to 24.0psi @17:40:47.78 SS.reconfigure SSinhale.with maxSpeed: 110 @17:40:47.91 SS.seek 25ml @17:40:50.64 Vacuum too high -- waiting up to 40s @17:40:54.01 Intake @31.2psia, vacuum from 20.0 to 24.0psi @17:40:54.04 SS.reconfigure SSinhale.with maxSpeed: 99 @17:40:54.13 SS.seek 25ml @17:40:56.65 Vacuum too high -- waiting up to 40s @17:40:59.73 Intake @31.1psia, vacuum from 20.0 to 24.0psi @17:40:59.76 SS.reconfigure SSinhale.with maxSpeed: 89 @17:40:59.85 SS.seek 25ml @17:41:01.94 Vacuum too high -- waiting up to 40s @17:41:05.27 SS.reconfigure SSinhale.with maxSpeed: 80 @17:41:05.35 SS.seek 25ml @17:41:07.53 Vacuum too high -- waiting up to 40s @17:41:10.99 Intake @31.2psia, vacuum from 20.0 to 24.0psi @17:41:11.02 SS.reconfigure SSinhale.with maxSpeed: 72 @17:41:11.13 SS.seek 25ml @17:41:13.40 Vacuum too high -- waiting up to 40s @17:41:16.53 Intake @31.2psia, vacuum from 20.0 to 24.0psi @17:41:16.56 SS.reconfigure SSinhale.with maxSpeed: 65 @17:41:16.66 SS.seek 25ml @17:41:19.22 Vacuum too high -- waiting up to 40s @17:41:22.30 Intake @31.0psia, vacuum from 20.0 to 24.0psi @17:41:22.32 SS.reconfigure SSinhale.with maxSpeed: 59 @17:41:22.42 SS.seek 25ml @17:41:25.12 Vacuum too high -- waiting up to 40s @17:41:28.44 Intake @31.2psia, vacuum from 20.0 to 24.0psi @17:41:28.46 SS.reconfigure SSinhale.with maxSpeed: 53 @17:41:28.56 SS.seek 25ml @17:41:31.56 Vacuum too high -- waiting up to 40s @17:41:35.01 Intake @31.1psia, vacuum from 20.0 to 24.0psi @17:41:35.04 SS.reconfigure SSinhale.with maxSpeed: 48 @17:41:35.13 SS.seek 25ml @17:41:39.36 Vacuum too high -- waiting up to 40s @17:41:42.48 Intake @31.1psia, vacuum from 20.0 to 24.0psi @17:41:42.51 SS.reconfigure SSinhale.with maxSpeed: 43 @17:41:42.60 SS.seek 25ml @17:41:44.56 Stroke completed -- waiting for pressure to equalize ... @17:41:50.90 Intake.select :close @17:41:51.08 SSV.dial :exhaust @17:41:51.93 Exhaust.select :open @17:41:52.08 SS.reconfigure SSexhale @17:41:52.15 SS.seek 0ml @17:42:01.20 Exhaust.select :close @17:42:01.35 SSV.dial CBV @17:42:01.38 CBV.dial SSV @17:42:02.25 Allowing 130s for 25.0ml of remaining 1475.0ml @17:42:02.27 Intake.select :open @17:42:04.49 Intake @31.2psia, vacuum from 20.0 to 24.0psi @17:42:04.52 SS.reconfigure SSinhale.with maxSpeed: 43 @17:42:04.61 SS.seek 25ml @17:42:10.64 Vacuum too high -- waiting up to 40s @17:42:12.97 Intake @31.1psia, vacuum from 20.0 to 24.0psi @17:42:13.00 SS.reconfigure SSinhale.with maxSpeed: 39 @17:42:13.09 SS.seek 25ml @17:42:18.24 Vacuum too high -- waiting up to 40s @17:42:21.44 Intake @31.0psia, vacuum from 20.0 to 24.0psi @17:42:21.47 SS.reconfigure SSinhale.with maxSpeed: 35 @17:42:21.57 SS.seek 25ml @17:42:28.87 Vacuum too high -- waiting up to 40s @17:42:31.74 SS.reconfigure SSinhale.with maxSpeed: 32 @17:42:31.83 SS.seek 25ml @17:42:39.56 Vacuum too high -- waiting up to 40s @17:42:42.39 SS.reconfigure SSinhale.with maxSpeed: 29 @17:42:42.47 SS.seek 25ml @17:42:51.15 Vacuum too high -- waiting up to 40s @17:42:54.23 SS.reconfigure SSinhale.with maxSpeed: 26 @17:42:54.31 SS.seek 25ml @17:43:08.55 Vacuum too high -- waiting up to 40s @17:43:11.65 SS.reconfigure SSinhale.with maxSpeed: 23 @17:43:11.73 SS.seek 25ml @17:43:24.40 Stroke completed -- waiting for pressure to equalize ... @17:43:31.43 Intake.select :close @17:43:31.62 SSV.dial :exhaust @17:43:32.46 Exhaust.select :open @17:43:32.61 SS.reconfigure SSexhale @17:43:32.68 SS.seek 0ml @17:43:41.85 Exhaust.select :close @17:43:42.00 SSV.dial CBV @17:43:42.03 CBV.dial SSV @17:43:42.88 Allowing 130s for 25.0ml of remaining 1450.0ml @17:43:42.90 Intake.select :open @17:43:45.12 Intake @31.1psia, vacuum from 20.0 to 24.0psi @17:43:45.15 SS.reconfigure SSinhale.with maxSpeed: 23 @17:43:45.24 SS.seek 25ml @17:44:20.46 Vacuum too high -- waiting up to 40s @17:44:23.57 Intake @30.9psia, vacuum from 20.0 to 24.0psi @17:44:23.59 SS.reconfigure SSinhale.with maxSpeed: 21 @17:44:23.69 SS.seek 25ml @17:45:09.12 Stroke completed -- waiting for pressure to equalize ... @17:45:19.18 Intake.select :close @17:45:19.36 SSV.dial :exhaust @17:45:20.20 Exhaust.select :open @17:45:20.35 SS.reconfigure SSexhale @17:45:20.42 SS.seek 0ml @17:45:29.54 Exhaust.select :close @17:45:29.69 SSV.dial CBV @17:45:29.72 CBV.dial SSV @17:45:31.20 Allowing 130s for 25.0ml of remaining 1425.0ml @17:45:31.22 Intake.select :open @17:45:33.44 Intake @31.1psia, vacuum from 20.0 to 24.0psi @17:45:33.47 SS.reconfigure SSinhale.with maxSpeed: 21 @17:45:33.57 SS.seek 25ml @17:46:29.44 Vacuum too high -- waiting up to 40s @17:46:32.80 Intake @30.8psia, vacuum from 20.0 to 24.0psi @17:46:32.83 SS.reconfigure SSinhale.with maxSpeed: 19 @17:46:32.92 SS.seek 25ml @17:47:03.62 Stroke completed -- waiting for pressure to equalize ... @17:47:13.05 Intake.select :close @17:47:13.24 SSV.dial :exhaust @17:47:14.10 Exhaust.select :open @17:47:14.25 SS.reconfigure SSexhale @17:47:14.32 SS.seek 0ml @17:47:23.26 Exhaust.select :close @17:47:23.41 SSV.dial CBV @17:47:23.44 CBV.dial SSV @17:47:24.29 Allowing 130s for 25.0ml of remaining 1400.0ml @17:47:24.31 Intake.select :open @17:47:26.52 Intake @31.0psia, vacuum from 20.0 to 24.0psi @17:47:26.54 SS.reconfigure SSinhale.with maxSpeed: 19 @17:47:26.64 SS.seek 25ml @17:48:58.94 Stroke completed -- waiting for pressure to equalize ... @17:49:08.98 Intake.select :close @17:49:09.17 SSV.dial :exhaust @17:49:10.02 Exhaust.select :open @17:49:10.17 SS.reconfigure SSexhale @17:49:10.24 SS.seek 0ml @17:49:19.17 Exhaust.select :close @17:49:19.32 SSV.dial CBV @17:49:19.35 CBV.dial SSV @17:49:20.18 Allowing 130s for 25.0ml of remaining 1375.0ml @17:49:20.20 Intake.select :open @17:49:22.41 Intake @31.1psia, vacuum from 20.0 to 24.0psi @17:49:22.44 SS.reconfigure SSinhale.with maxSpeed: 19 @17:49:22.54 SS.seek 25ml @17:50:54.93 Stroke completed -- waiting for pressure to equalize ... @17:51:05.04 Intake.select :close @17:51:05.23 SSV.dial :exhaust @17:51:06.07 Exhaust.select :open @17:51:06.22 SS.reconfigure SSexhale @17:51:06.29 SS.seek 0ml @17:51:15.56 Exhaust.select :close @17:51:15.71 SSV.dial CBV @17:51:15.74 CBV.dial SSV @17:51:16.57 Allowing 130s for 25.0ml of remaining 1350.0ml @17:51:16.59 Intake.select :open @17:51:18.81 Intake @31.1psia, vacuum from 20.0 to 24.0psi @17:51:18.83 SS.reconfigure SSinhale.with maxSpeed: 19 @17:51:18.93 SS.seek 25ml @17:52:51.53 Stroke completed -- waiting for pressure to equalize ... @17:53:02.83 Intake.select :close @17:53:03.01 SSV.dial :exhaust @17:53:03.86 Exhaust.select :open @17:53:04.01 SS.reconfigure SSexhale @17:53:04.08 SS.seek 0ml @17:53:13.89 Exhaust.select :close @17:53:14.04 SSV.dial CBV @17:53:14.07 CBV.dial SSV @17:53:14.92 Allowing 130s for 25.0ml of remaining 1325.0ml @17:53:14.94 Intake.select :open @17:53:17.15 Intake @31.0psia, vacuum from 20.0 to 24.0psi @17:53:17.18 SS.reconfigure SSinhale.with maxSpeed: 19 @17:53:17.27 SS.seek 25ml @17:54:49.76 Stroke completed -- waiting for pressure to equalize ... @17:55:01.76 Intake.select :close @17:55:01.95 SSV.dial :exhaust @17:55:02.79 Exhaust.select :open @17:55:02.94 SS.reconfigure SSexhale @17:55:03.01 SS.seek 0ml @17:55:12.09 Exhaust.select :close @17:55:12.24 SSV.dial CBV @17:55:12.27 CBV.dial SSV @17:55:13.12 Allowing 130s for 25.0ml of remaining 1300.0ml @17:55:13.14 Intake.select :open @17:55:15.34 Intake @31.1psia, vacuum from 20.0 to 24.0psi @17:55:15.37 SS.reconfigure SSinhale.with maxSpeed: 19 @17:55:15.46 SS.seek 25ml @17:56:25.62 Vacuum too high -- waiting up to 40s @17:56:29.20 Intake @30.8psia, vacuum from 20.0 to 24.0psi @17:56:29.23 SS.reconfigure SSinhale.with maxSpeed: 17 @17:56:29.32 SS.seek 25ml @17:56:54.61 Stroke completed -- waiting for pressure to equalize ... @17:57:02.25 Session Ended @17:57:04.71 Intake.select :close @17:57:04.87 SSV.dial :exhaust @17:57:05.70 Exhaust.select :open @17:57:05.82 SS.reconfigure SSexhale @17:57:05.87 SS.seek 0ml @17:57:14.86 Exhaust.select :close @17:57:14.98 SSV.dial CBV @17:57:15.00 CBV.dial SSV @17:57:15.84 Allowing 130s for 25.0ml of remaining 1275.0ml @17:57:15.85 Intake.select :open @17:57:18.03 Intake @31.1psia, vacuum from 20.0 to 24.0psi @17:57:18.05 SS.reconfigure SSinhale.with maxSpeed: 17 @17:57:18.10 SS.seek 25ml @17:59:01.78 Stroke completed -- waiting for pressure to equalize ... @17:59:12.45 Intake.select :close @17:59:12.61 SSV.dial :exhaust @17:59:13.44 Exhaust.select :open @17:59:13.56 SS.reconfigure SSexhale @17:59:13.60 SS.seek 0ml @17:59:22.73 Exhaust.select :close @17:59:22.85 SSV.dial CBV @17:59:22.87 CBV.dial SSV @17:59:23.70 Allowing 130s for 25.0ml of remaining 1250.0ml @17:59:23.71 Intake.select :open @17:59:25.88 Intake @31.1psia, vacuum from 20.0 to 24.0psi @17:59:25.90 SS.reconfigure SSinhale.with maxSpeed: 17 @17:59:25.95 SS.seek 25ml @18:01:09.22 Stroke completed -- waiting for pressure to equalize ... @18:01:21.75 Intake.select :close @18:01:21.91 SSV.dial :exhaust @18:01:22.73 Exhaust.select :open @18:01:22.85 SS.reconfigure SSexhale @18:01:22.90 SS.seek 0ml @18:01:32.09 Exhaust.select :close @18:01:32.21 SSV.dial CBV @18:01:32.23 CBV.dial SSV @18:01:33.06 Allowing 130s for 25.0ml of remaining 1225.0ml @18:01:33.07 Intake.select :open @18:01:35.26 Intake @31.1psia, vacuum from 20.0 to 24.0psi @18:01:35.28 SS.reconfigure SSinhale.with maxSpeed: 17 @18:01:35.32 SS.seek 25ml @18:02:49.50 Vacuum too high -- waiting up to 40s @18:02:53.58 Intake @30.8psia, vacuum from 20.0 to 24.0psi @18:02:53.60 SS.reconfigure SSinhale.with maxSpeed: 15 @18:02:53.65 SS.seek 25ml @18:03:26.98 Stroke completed -- waiting for pressure to equalize ... @18:03:38.87 Intake.select :close @18:03:39.03 SSV.dial :exhaust @18:03:39.86 Exhaust.select :open @18:03:39.98 SS.reconfigure SSexhale @18:03:40.02 SS.seek 0ml @18:03:49.33 Exhaust.select :close @18:03:49.45 SSV.dial CBV @18:03:49.47 CBV.dial SSV @18:03:50.89 Allowing 130s for 25.0ml of remaining 1200.0ml @18:03:50.90 Intake.select :open @18:03:53.09 Intake @31.1psia, vacuum from 20.0 to 24.0psi @18:03:53.11 SS.reconfigure SSinhale.with maxSpeed: 15 @18:03:53.16 SS.seek 25ml @18:05:50.08 Stroke completed -- waiting for pressure to equalize ... @18:06:00.91 SS.reconfigure SSconfig @18:06:00.96 SS.hold @18:06:01.09 Intake.select :close @18:06:01.25 ShallowSampler::Clogged in WCR -- progress too slow after 325.0ml @18:06:01.26 SSV.dial :exhaust @18:06:02.09 Exhaust.select :open @18:06:02.21 SS.reconfigure SSexhale @18:06:02.26 SS.seek 0ml @18:06:11.77 Exhaust.select :close @18:06:11.88 Sampled 325.0ml @18:06:11.90 Drying puck before fixing for 4:00 @18:06:11.91 CBV.dial :waste2b @18:06:12.73 delay 1:00 @18:07:12.76 CSV.dial :air @18:07:13.54 CS.seek 10ml @18:07:37.37 Pumping wcr puck from tube 3 up to 55psi... @18:07:37.38 CBV.dial SSV @18:07:38.76 CBV.dial between CSV and waste2b @18:07:39.80 CSV.dial between air and CTV @18:07:40.13 CS.seek 7.122ml @18:07:47.79 CTV.dial CSV,avoiding: :air @18:07:47.81 CSV.dial CTV @18:07:48.67 CS.reconfigure CSpressurize @18:07:48.73 CS.seek 0ml @18:08:07.59 CSV.dial :air @18:08:08.09 CS.reconfigure CSfast @18:08:08.14 CS.seek 10ml @18:08:31.84 CSV.dial between air and CTV @18:08:32.20 CS.seek 2.529ml @18:08:50.34 CTV.dial CSV,avoiding: :air @18:08:50.37 CSV.dial CTV @18:08:50.71 CS.reconfigure CSpressurize @18:08:50.76 CS.seek 0ml @18:08:55.18 CBV.dial :waste2b @18:08:55.53 CS.reconfigure CSconfig @18:08:55.58 CS.seek :empty @18:08:59.03 delay 1:00 @18:09:59.06 CTV.dial :air,avoiding: :intake @18:10:01.03 Fix #1 of 2 (with rnal) for 2:00 @18:10:01.05 CSV.dial CSR @18:10:01.58 Pulling 2ml of CSR.rnal @18:10:01.60 CSR.select :rnal @18:10:01.72 CS.seek 2ml @18:10:07.84 delay 5 seconds @18:10:12.87 CSR.select :air @18:10:13.00 CS.reconfigure CSfast @18:10:13.05 CS.seek 10ml @18:10:32.25 Pumping wcr puck from tube 3 up to 55psi... @18:10:32.27 CBV.dial SSV @18:10:33.64 CBV.dial between CSV and waste2b @18:10:34.68 CSV.dial between air and CTV @18:10:35.32 CS.seek 7.266ml @18:10:42.54 CTV.dial CSV,avoiding: :air @18:10:42.56 CSV.dial CTV @18:10:44.06 CS.reconfigure CSpressurize @18:10:44.11 CS.seek 0ml @18:11:03.25 CSV.dial :air @18:11:03.74 CS.reconfigure CSfast @18:11:03.80 CS.seek 10ml @18:11:27.37 CSV.dial between air and CTV @18:11:27.73 CS.seek 2.131ml @18:11:46.92 CTV.dial CSV,avoiding: :air @18:11:46.94 CSV.dial CTV @18:11:47.29 CS.reconfigure CSpressurize @18:11:47.34 CS.seek 0ml @18:11:49.12 delay 2:00 @18:13:49.15 CBV.dial :waste2b @18:13:49.51 CS.reconfigure CSconfig @18:13:49.57 CS.seek :empty @18:13:54.69 delay 1:00 @18:14:54.72 CTV.dial :air,avoiding: :intake @18:14:56.12 Fix #2 of 2 (with rnal) for 2:00 @18:14:56.14 CSV.dial CSR @18:14:56.66 Pulling 2ml of CSR.rnal @18:14:56.67 CSR.select :rnal @18:14:56.80 CS.seek 2ml @18:15:02.98 delay 5 seconds @18:15:08.01 CSR.select :air @18:15:08.14 CS.reconfigure CSfast @18:15:08.18 CS.seek 10ml @18:15:27.29 Pumping wcr puck from tube 3 up to 55psi... @18:15:27.31 CBV.dial SSV @18:15:28.68 CBV.dial between CSV and waste2b @18:15:29.72 CSV.dial between air and CTV @18:15:30.36 CS.seek 7.261ml @18:15:37.67 CTV.dial CSV,avoiding: :air @18:15:37.74 CSV.dial CTV @18:15:39.12 CS.reconfigure CSpressurize @18:15:39.17 CS.seek 0ml @18:15:58.41 CSV.dial :air @18:15:58.88 CS.reconfigure CSfast @18:15:59.51 CS.seek 10ml @18:16:23.14 CSV.dial between air and CTV @18:16:23.49 CS.seek 2.246ml @18:16:42.33 CTV.dial CSV,avoiding: :air @18:16:42.35 CSV.dial CTV @18:16:42.69 CS.reconfigure CSpressurize @18:16:42.74 CS.seek 0ml @18:16:45.19 delay 2:00 @18:18:45.23 CBV.dial :waste2b @18:18:45.57 CS.reconfigure CSconfig @18:18:45.63 CS.seek :empty @18:18:50.37 delay 1:00 @18:19:50.40 CTV.dial :air,avoiding: :intake @18:19:51.85 Storing wcr puck in Collection Clamp to tube 2 @18:19:52.04 SC.reconfigure SCtight @18:19:52.09 SC.seek 2 @18:19:54.28 SC.reconfigure SCloose @18:19:54.45 SE.reconfigure SEplace @18:19:54.54 Elbow.reconfigure TightElbow @18:19:54.59 Elbow.seek CC @18:19:54.64 SE.seek beamBreak @18:19:54.87 Elbow.reconfigure LooseElbow @18:19:54.98 Forearm.seek CC @18:19:56.05 Hand.close @18:19:56.70 CC.seek :open @18:19:57.49 SE.configure SEconfig @18:19:57.55 SE.seek :place @18:20:01.21 Forearm.seek :retract @18:20:02.26 Elbow.reconfigure TightElbow @18:20:02.31 Elbow.seek 2 @18:20:04.20 Elbow.reconfigure LimpElbow @18:20:04.25 Forearm.seek 2 @18:20:05.26 Elbow.hold @18:20:05.34 Elbow.reconfigure TightElbow @18:20:05.39 Hand.open @18:20:06.21 Forearm.force -20 @18:20:06.26 Forearm.seek 2 + 343 counts @18:20:06.56 Forearm.reconfigure TightForearm @18:20:06.61 Forearm.seek 2 + 700 counts @18:20:07.08 SE.seek :down @18:20:08.24 Forearm.reconfigure LooseForearm @18:20:08.29 Forearm.seek :retract @18:20:09.88 SE.coast @18:20:09.92 SC.coast @18:20:09.96 Starting finalPurge @18:20:09.98 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:20:10.05 Hand.open @18:20:11.04 Elbow.seek FlushPuck::Garage @18:20:13.07 Elbow.reconfigure LooseElbow @18:20:13.18 Forearm.seek :garage @18:20:14.20 Hand.close @18:20:14.95 Forearm.seek :retract @18:20:15.95 Elbow.reconfigure TightElbow @18:20:16.00 Elbow.seek CC @18:20:18.95 Elbow.reconfigure LooseElbow @18:20:19.06 Forearm.reconfigure TightForearm @18:20:19.11 Forearm.seek CC @18:20:20.46 CC.seek :closed @18:20:24.73 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.90 @18:20:24.80 Hand.open @18:20:25.52 Forearm.reconfigure LooseForearm @18:20:25.56 Forearm.seek :retract @18:20:26.57 Flushing the Collection Valve::Manifold @18:20:26.71 CSV.dial CSR @18:20:27.23 Pulling 5ml of CSR.flush @18:20:27.24 CSR.select :flush @18:20:27.37 CS.seek 5ml @18:20:41.20 delay 5 seconds @18:20:46.23 CSR.select :air @18:20:46.39 CS.reconfigure CSfast @18:20:46.44 CS.seek 7.5ml @18:20:53.17 CSV.dial :waste @18:20:54.51 CS.reconfigure CSconfig @18:20:54.56 CS.seek 0ml @18:21:14.73 Flushing lower manifold's waste1 port @18:21:14.86 CSV.dial :flush @18:21:15.33 CS.reconfigure CSfast @18:21:15.38 CS.seek 5ml @18:21:27.64 delay 5 seconds @18:21:32.67 CSV.dial :air @18:21:33.21 CS.seek 7ml @18:21:38.89 CSV.dial CTV @18:21:38.93
CTV.dial CSV @18:21:40.39 CBV.dial :waste1 @18:21:41.21 CS.seek 0ml @18:21:57.92 Flushing lower manifold's waste2b port @18:21:58.05 CSV.dial :flush @18:21:58.84 CS.seek 5ml @18:22:11.23 delay 5 seconds @18:22:16.26 CSV.dial :air @18:22:16.82 CS.seek 7ml @18:22:22.36 CSV.dial CTV @18:22:22.40
CTV.dial CSV @18:22:22.86 CBV.dial :waste2b @18:22:23.68 CS.seek 0ml @18:22:40.48 Flushing upper manifold @18:22:40.61 CSV.dial :flush @18:22:41.40 CS.seek 5ml @18:22:53.67 delay 5 seconds @18:22:58.70 CSV.dial :air @18:22:59.85 CS.seek 7ml @18:23:05.52 CSV.dial CBV @18:23:05.55
CBV.dial CSV @18:23:06.69 CTV.dial :air @18:23:08.09 CS.seek 0ml @18:23:24.80 delay 7 seconds @18:23:31.84 CS.seek 3ml @18:23:39.77 CSV.dial :waste @18:23:40.26 CS.seek 0ml @18:23:48.23 CSV.dial :flush @18:23:48.72 CS.seek 3ml @18:23:56.52 delay 5 seconds @18:24:01.55 CSV.dial :air @18:24:02.09 CS.seek 5ml @18:24:07.67 CSV.dial :air @18:24:07.69 CS.seek 0ml @18:24:20.02 delay 7 seconds @18:24:27.06 CS.seek 3ml @18:24:34.86 CSV.dial :waste @18:24:35.64 CS.seek 0ml @18:24:43.52 Disinfecting external Intake with mfbkill @18:24:43.65 CSV.dial CSR @18:24:45.06 Pulling 8ml of CSR.mfbkill @18:24:45.07 CSR.select :mfbkill @18:24:45.20 CS.seek 8ml @18:25:04.24 delay 5 seconds @18:25:09.27 CSR.select :air @18:25:09.39 CS.seek 10ml @18:25:14.93 CSV.dial CBV @18:25:14.96
CBV.dial CSV @18:25:16.05 CTV.dial :intake @18:25:16.85 Intake.select :open @18:25:16.97 CS.seek 2ml @18:25:37.27 Intake.select :close @18:25:37.38 CTV.dial :air @18:25:38.18 CS.reconfigure CSconfig @18:25:38.23 CS.seek 0ml @18:25:44.36 Flushing lower manifold's waste1 port @18:25:44.37 CSV.dial CSR @18:25:45.50 Pulling 4ml of CSR.mfbkill @18:25:45.51 CSR.select :mfbkill @18:25:45.64 CS.seek 4ml @18:25:56.83 delay 5 seconds @18:26:01.86 CSR.select :air @18:26:01.98 CS.reconfigure CSfast @18:26:02.03 CS.seek 10ml @18:26:16.65 CSV.dial CTV @18:26:16.68
CTV.dial CSV @18:26:18.16 CBV.dial :waste1 @18:26:18.66 CS.seek 0ml @18:26:42.18 Flushing lower manifold's waste2b port @18:26:42.19 CSV.dial CSR @18:26:42.72 Pulling 4ml of CSR.mfbkill @18:26:42.73 CSR.select :mfbkill @18:26:42.86 CS.reconfigure CSconfig @18:26:42.90 CS.seek 4ml @18:26:54.12 delay 5 seconds @18:26:59.15 CSR.select :air @18:26:59.28 CS.reconfigure CSfast @18:26:59.32 CS.seek 10ml @18:27:14.02 CSV.dial CTV @18:27:14.06
CTV.dial CSV @18:27:14.54 CBV.dial :waste2b @18:27:15.36 CS.seek 0ml @18:27:38.87 Killing upper manifold @18:27:38.88 CSV.dial CSR @18:27:39.43 Pulling 4ml of CSR.mfbkill @18:27:39.44 CSR.select :mfbkill @18:27:39.56 CS.reconfigure CSconfig @18:27:39.60 CS.seek 4ml @18:27:50.87 delay 5 seconds @18:27:55.90 CSR.select :air @18:27:56.03 CS.reconfigure CSfast @18:27:56.07 CS.seek 10ml @18:28:10.65 CSV.dial CBV @18:28:10.69
CBV.dial CSV @18:28:12.37 CTV.dial :air @18:28:13.78 CS.seek 0ml @18:28:37.27 delay 7 seconds @18:28:44.31 CS.seek 3ml @18:28:52.15 CSV.dial :waste @18:28:52.63 CS.seek 0ml @18:29:00.43 Flushing upper manifold @18:29:00.57 CSV.dial :flush @18:29:01.03 CS.seek 5ml @18:29:13.31 delay 5 seconds @18:29:18.34 CSV.dial :air @18:29:19.00 CS.seek 7ml @18:29:24.59 CSV.dial CBV @18:29:24.62
CBV.dial CSV @18:29:25.70 CTV.dial :air @18:29:25.72 CS.seek 0ml @18:29:42.62 delay 7 seconds @18:29:49.66 CS.seek 3ml @18:29:57.46 CSV.dial :waste @18:29:57.94 CS.seek 0ml @18:30:06.05 CSV.dial :flush @18:30:06.52 CS.seek 3ml @18:30:14.28 delay 5 seconds @18:30:19.31 CSV.dial :air @18:30:19.88 CS.seek 5ml @18:30:25.53 CSV.dial :air @18:30:25.55 CS.seek 0ml @18:30:37.90 delay 7 seconds @18:30:44.94 CS.seek 3ml @18:30:52.84 CSV.dial :waste @18:30:53.61 CS.seek 0ml @18:31:01.40 Flushing lower manifold's waste1 port @18:31:01.54 CSV.dial :flush @18:31:02.02 CS.seek 5ml @18:31:14.34 delay 5 seconds @18:31:19.37 CSV.dial :air @18:31:19.91 CS.seek 7ml @18:31:25.46 CSV.dial CTV @18:31:25.50
CTV.dial CSV @18:31:26.96 CBV.dial :waste1 @18:31:27.46 CS.seek 0ml @18:31:44.25 Flushing lower manifold's waste2b port @18:31:44.38 CSV.dial :flush @18:31:45.18 CS.seek 5ml @18:31:57.40 delay 5 seconds @18:32:02.43 CSV.dial :air @18:32:02.97 CS.seek 7ml @18:32:08.50 CSV.dial CTV @18:32:08.52
CTV.dial CSV @18:32:09.00 CBV.dial :waste2b @18:32:09.82 CS.seek 0ml @18:32:26.56 Disinfecting external Intake with kill @18:32:26.69 CSV.dial CSR @18:32:27.21 Pulling 8ml of CSR.kill @18:32:27.22 CSR.select :kill @18:32:27.35 CS.seek 8ml @18:32:46.37 delay 5 seconds @18:32:51.41 CSR.select :air @18:32:51.53 CS.seek 10ml @18:32:57.19 CSV.dial CBV @18:32:57.23
CBV.dial CSV @18:32:58.34 CTV.dial :intake @18:32:59.11 Intake.select :open @18:32:59.23 CS.seek 2ml @18:33:19.31 Intake.select :close @18:33:19.42 CTV.dial :air @18:33:20.22 CS.reconfigure CSconfig @18:33:20.27 CS.seek 0ml @18:33:26.44 CSV.dial CSR @18:33:28.16 Pulling 5ml of CSR.flush @18:33:28.17 CSR.select :flush @18:33:28.29 CS.seek 5ml @18:33:42.00 delay 5 seconds @18:33:47.03 CSR.select :air @18:33:47.15 CS.seek 10ml @18:34:00.97 CSV.dial CBV @18:34:02.06 CS.seek 0ml @18:34:28.56 Garaging FlushPuck in Collection Clamp @18:34:28.64 Elbow.reconfigure TightElbow @18:34:28.69 Elbow.seek CC @18:34:28.92 Elbow.reconfigure LooseElbow @18:34:29.03 Forearm.seek CC @18:34:30.04 Hand.close @18:34:30.60 CC.seek :open @18:34:34.97 Forearm.seek :retract @18:34:35.99 Elbow.reconfigure TightElbow @18:34:36.04 Elbow.seek :garage @18:34:39.10 Elbow.reconfigure LooseElbow @18:34:39.21 Forearm.reconfigure TightForearm @18:34:39.26 Forearm.seek :garage @18:34:40.41 Hand.open @18:34:41.04 Forearm.reconfigure LooseForearm @18:34:41.09 Forearm.seek :retract @18:34:42.11 Starting initialPurge @18:34:42.12 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @18:34:42.20 Hand.open @18:34:42.54 Elbow.reconfigure TightElbow @18:34:42.58 Elbow.seek FlushPuck::Garage @18:34:42.71 Elbow.reconfigure LooseElbow @18:34:42.82 Forearm.seek :garage @18:34:43.83 Hand.close @18:34:44.60 Forearm.seek :retract @18:34:45.63 Elbow.reconfigure TightElbow @18:34:45.68 Elbow.seek CC @18:34:48.61 Elbow.reconfigure LooseElbow @18:34:48.71 Forearm.reconfigure TightForearm @18:34:48.76 Forearm.seek CC @18:34:50.09 CC.seek :closed @18:34:54.34 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.93 @18:34:54.41 Hand.open @18:34:55.04 Forearm.reconfigure LooseForearm @18:34:55.08 Forearm.seek :retract @18:34:56.11 Flushing the Collection Valve::Manifold @18:34:56.24 CSV.dial CSR @18:34:57.37 Pulling 5ml of CSR.flush @18:34:57.38 CSR.select :flush @18:34:57.50 CS.seek 5ml @18:35:11.31 delay 5 seconds @18:35:16.34 CSR.select :air @18:35:16.51 CS.reconfigure CSfast @18:35:16.56 CS.seek 7.501ml @18:35:23.34 CSV.dial :waste @18:35:24.69 CS.reconfigure CSconfig @18:35:24.74 CS.seek 0ml @18:35:45.03 Purging external Intake @18:35:45.16 CSV.dial :flush @18:35:45.64 CS.reconfigure CSfast @18:35:45.69 CS.seek 10ml @18:36:09.69 delay 5 seconds @18:36:14.72 CSV.dial CBV @18:36:14.76
CBV.dial CSV @18:36:15.55 CTV.dial :intake @18:36:16.36 Intake.select :open @18:36:16.48 CS.seek 0ml @18:36:41.56 Intake.select :close @18:36:41.67 CTV.dial :air @18:36:42.47 Flushing upper manifold @18:36:42.60 CSV.dial :flush @18:36:43.39 CS.seek 5ml @18:36:55.85 delay 5 seconds @18:37:00.88 CSV.dial :air @18:37:01.42 CS.seek 7.001ml @18:37:07.00 CSV.dial CBV @18:37:07.02
CBV.dial CSV @18:37:08.11 CTV.dial :air @18:37:08.13 CS.seek 0ml @18:37:24.82 delay 7 seconds @18:37:31.86 CS.seek 3ml @18:37:39.78 CSV.dial :waste @18:37:40.26 CS.seek 0ml @18:37:48.22 CSV.dial :flush @18:37:48.68 CS.seek 3ml @18:37:56.41 delay 5 seconds @18:38:01.44 CSV.dial :air @18:38:01.98 CS.seek 5ml @18:38:07.47 CSV.dial :air @18:38:07.49 CS.seek 0ml @18:38:19.79 delay 7 seconds @18:38:26.83 CS.seek 3ml @18:38:34.60 CSV.dial :waste @18:38:35.39 CS.seek 0ml @18:38:43.54 Garaging FlushPuck in Collection Clamp @18:38:43.62 Elbow.reconfigure TightElbow @18:38:43.67 Elbow.seek CC @18:38:43.92 Elbow.reconfigure LooseElbow @18:38:44.02 Forearm.seek CC @18:38:45.03 Hand.close @18:38:45.61 CC.seek :open @18:38:49.95 Forearm.seek :retract @18:38:50.95 Elbow.reconfigure TightElbow @18:38:51.00 Elbow.seek :garage @18:38:54.23 Elbow.reconfigure LooseElbow @18:38:54.33 Forearm.reconfigure TightForearm @18:38:54.38 Forearm.seek :garage @18:38:55.53 Hand.open @18:38:56.18 Forearm.reconfigure LooseForearm @18:38:56.22 Forearm.seek :retract @18:38:57.27 Completed [wcrphase] begun 17:34:53 @18:38:57.29 Preparing to sleep until 22:00 20-may-20 @18:38:57.36 SS.coast @18:38:57.38
Asleep til 22:00 20-may-20 @18:38:57.45 CC.coast @18:38:57.49
Continuing at 22:00 @18:38:57.54 CS.coast @18:38:57.65 PC.coast @18:38:57.68 GoodNews.email "Then, in tube 3: wcrphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:137\n{1 => 22, 2 => 1, 3 => 21}\nCan@18:38:57, 9.2C, 45% humidity, 14.4psia, 13.564V, 0.424A, 0.430A avg, 5.75W",Subject: "Asleep until 22:00" @18:38:57.76 Forearm.coast @18:38:57.80 Elbow.coast @18:38:58.19 Gate.power :core,:OFF @18:38:58.21 Gate.power :raw,:OFF @18:38:58.22 Shutdown @22:00:00.06
Powering core bus @22:00:05.18 Gate.power :raw,:ON @22:00:05.84 SPE.coast @22:00:05.91 SPE.configure SPEconfig @22:00:06.00 AS.configure ASconfig @22:00:06.11 SS.configure SSconfig @22:00:06.21 CC.configure CCconfig @22:00:06.30 CS.configure CSconfig @22:00:06.40 PC.configure PCconfig @22:00:06.51 PS.configure PSconfig @22:00:06.63 Forearm.configure LooseForearm @22:00:06.72 Elbow.configure LooseElbow @22:00:06.84 SC.configure SCloose @22:00:06.94 SE.configure SEconfig @22:00:06.99 CH.coast @22:00:07.04 CH.configure CHconfig @22:00:07.08 PH.coast @22:00:07.14 PH.configure PHconfig @22:00:07.18 PCR.off @22:00:07.21 PCR.pidtemp= 50,0.1,0,300,25,25 @22:00:07.24 PCR.caltemp= 2.8814,0.0372,0 @22:00:07.27 All dwarf controllers running firmware version 3.75 @22:00:07.38 Valve.reset @22:00:07.55 PCR.off @22:00:07.59 PCR.pidtemp= 50,0.1,0,300,25,25 @22:00:07.62 PCR.caltemp= 2.8814,0.0372,0 @22:00:07.85 Intake.select :close @22:00:07.86 Exhaust.select :close @22:00:07.87 CTV.dial :air @22:00:07.95 PSV.dial :air @22:00:08.11 CSV.dial :waste1 @22:00:08.14
Forearm.reconfigure LooseForearm @22:00:08.16 PS.reconfigure PSconfig @22:00:08.31 PS.home.seek 0ml @22:00:08.33
Forearm.home.seek :retract @22:00:08.43 SSV.dial :spit,avoiding: :exhaust @22:00:09.13 Hand.close @22:00:09.16
Elbow.jog 1500 @22:00:09.30 CS.reconfigure CSfast @22:00:09.33 SS.reconfigure SSconfig @22:00:09.43 SS.home.seek 0ml @22:00:09.46 CS.home.seek 0ml @22:00:09.88
Elbow.reconfigure LooseElbow @22:00:09.92 Elbow.home.seek :clear @22:00:13.41 SE.reconfigure SEconfig @22:00:13.46 SE.home.seek :down @22:00:14.08 SC.reconfigure SCsloppy @22:00:14.13 SC.home.seek :initial @22:00:17.40 PS.coast @22:00:18.11
Forearm.seek :retract @22:00:18.18 Elbow.seek :hammer @22:00:19.41 Forearm.reconfigure TightForearm @22:00:19.46 Forearm.seek 5 + 350 counts @22:00:20.48 Forearm.seek :retract @22:00:21.68 PC.reconfigure PCconfig @22:00:21.70
CC.reconfigure CCconfig @22:00:21.80 CC.home.seek :open @22:00:21.82 PC.home.seek :open @22:00:25.41
Phase [wcrphase] @22:00:25.42 GoodNews.email "At 22:00:25 began in tube 3: wcrphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:137\n{1 => 22, 2 => 1, 3 => 21}\nCan@22:00:25, 9.0C, 45% humidity, 14.3psia, 13.554V, 0.676A, 0.676A avg, 9.16W",Subject: "Started 20invasivedeployfinal phase wcrphase" @22:00:25.44CEST20-May-20
===> STARTED WCRSphase (phase #9) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @22:00:25.46 Starting initialPurge @22:00:25.50 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:00:25.58 Hand.open @22:00:26.34 Elbow.reconfigure TightElbow @22:00:26.39 Elbow.seek FlushPuck::Garage @22:00:28.36 Elbow.reconfigure LooseElbow @22:00:28.48 Forearm.reconfigure LooseForearm @22:00:28.53 Forearm.seek :garage @22:00:29.54 Hand.close @22:00:30.67 Forearm.seek :retract @22:00:31.69 Elbow.reconfigure TightElbow @22:00:31.74 Elbow.seek CC @22:00:34.75 Elbow.reconfigure LooseElbow @22:00:34.97 Forearm.reconfigure TightForearm @22:00:35.05 Forearm.seek CC @22:00:36.80 CC.seek :closed @22:00:41.26 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.07 @22:00:41.43 Hand.open @22:00:42.24 Forearm.reconfigure LooseForearm @22:00:42.33 Forearm.seek :retract @22:00:43.38 Flushing the Collection Valve::Manifold @22:00:43.72 CSV.dial CSR @22:00:45.25 Pulling 5ml of CSR.flush @22:00:45.27 CSR.select :flush @22:00:45.45 CS.reconfigure CSconfig @22:00:45.54 CS.seek 5ml @22:00:59.32 delay 5 seconds @22:01:04.35 CSR.select :air @22:01:04.62 CS.reconfigure CSfast @22:01:04.76 CS.seek 7.5ml @22:01:11.49 CSV.dial :waste @22:01:12.89 CS.reconfigure CSconfig @22:01:12.99 CS.seek 0ml @22:01:33.16 Purging external Intake @22:01:33.29 CSV.dial :flush @22:01:33.77 CS.reconfigure CSfast @22:01:33.82 CS.seek 10ml @22:01:57.51 delay 5 seconds @22:02:02.54 CSV.dial CBV @22:02:02.58
CBV.dial CSV @22:02:03.38 CTV.dial :intake @22:02:04.16 Intake.select :open @22:02:04.28 CS.seek 0ml @22:02:29.54 Intake.select :close @22:02:29.65 CTV.dial :air @22:02:30.46 Flushing upper manifold @22:02:30.59 CSV.dial :flush @22:02:31.38 CS.seek 5ml @22:02:43.73 delay 5 seconds @22:02:48.75 CSV.dial :air @22:02:49.87 CS.seek 7ml @22:02:55.51 CSV.dial CBV @22:02:55.53
CBV.dial CSV @22:02:56.62 CTV.dial :air @22:02:56.64 CS.seek 0ml @22:03:13.51 delay 7 seconds @22:03:20.55 CS.seek 3ml @22:03:28.35 CSV.dial :waste @22:03:28.83 CS.seek 0ml @22:03:36.82 CSV.dial :flush @22:03:37.30 CS.seek 3ml @22:03:45.26 delay 5 seconds @22:03:50.29 CSV.dial :air @22:03:50.83 CS.seek 5.001ml @22:03:56.54 CSV.dial :air @22:03:56.56 CS.seek 0ml @22:04:08.88 delay 7 seconds @22:04:15.92 CS.seek 3ml @22:04:23.85 CSV.dial :waste @22:04:24.63 CS.seek 0ml @22:04:32.45 Garaging FlushPuck in Collection Clamp @22:04:32.53 Elbow.reconfigure TightElbow @22:04:32.58 Elbow.seek CC @22:04:32.84 Elbow.reconfigure LooseElbow @22:04:32.95 Forearm.seek CC @22:04:33.92 Hand.close @22:04:34.59 CC.seek :open @22:04:38.95 Forearm.seek :retract @22:04:39.97 Elbow.reconfigure TightElbow @22:04:40.02 Elbow.seek :garage @22:04:43.20 Elbow.reconfigure LooseElbow @22:04:43.30 Forearm.reconfigure TightForearm @22:04:43.35 Forearm.seek :garage @22:04:44.50 Hand.open @22:04:45.16 Forearm.reconfigure LooseForearm @22:04:45.20 Forearm.seek :retract @22:04:46.25 Starting stand-alone bac WCR @22:04:46.26 bac wcr sampling at most 1500ml @22:04:46.29 BACfans.email "Started stand-alone bac WCR in tube 3 at 22:04:46CEST20-May-20\n{1 => 22, 2 => 1, 3 => 21}\nCan@22:00:25, 9.0C, 45% humidity, 14.3psia, 13.554V, 0.676A, 0.676A avg, 9.16W",Subject: "bac wcr sampling at most 1500ml" @22:04:46.32 Whole Cell Archive sampling 1500ml @22:04:46.36 Loading wcr puck from tube 3 into Collection Clamp @22:04:46.63 SC.reconfigure SCtight @22:04:46.69 SC.seek 3 @22:04:48.81 SC.reconfigure SCloose @22:04:49.06 Elbow.reconfigure TightElbow @22:04:49.12 Elbow.seek 3 @22:04:51.45 Forearm.reconfigure TightForearm @22:04:51.60 Forearm.seek 3 + 700 counts @22:04:52.69 SE.reconfigure SEpick @22:04:52.78 SE.seek beamBreak @22:04:54.83 SE.configure SEconfig @22:04:55.22 Elbow.seek 3 @22:04:55.85 Forearm.seek 3 + 300 counts @22:04:56.14 Forearm.reconfigure LooseForearm @22:04:56.26 Hand.close @22:04:57.07 SE.seek :down @22:04:57.62 Forearm.seek :retract @22:04:58.28 SE.coast @22:04:58.65 Elbow.seek CC @22:05:00.45 Elbow.reconfigure LooseElbow @22:05:00.75 Forearm.reconfigure TightForearm @22:05:00.88 Forearm.seek CC @22:05:02.66 CC.seek :closed @22:05:07.14 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.19 @22:05:07.17 Hand.open @22:05:08.01 Forearm.reconfigure LooseForearm @22:05:08.12 Forearm.seek :retract @22:05:09.21 SC.coast @22:05:09.29 collectSample 1500ml w/bubble point of 55psi @22:05:09.33 Priming 7ml @22:05:09.35 Intake.select :close @22:05:09.37 Exhaust.select :close @22:05:09.40 SS.hold @22:05:09.88 Calibrating pressure and temperature sensors @22:05:09.92 CTV.dial :air @22:05:09.95 SSV.dial :spit,avoiding: :exhaust @22:05:10.03 SS.reconfigure SSfast @22:05:10.12 SS.seek 5ml @22:05:12.89 SSV.dial CBV @22:05:12.96 CBV.dial SSV @22:05:14.45 delay 3.8 seconds @22:05:18.53 SSV.dial :spit @22:05:19.99 SS.seek 0ml @22:05:22.55 SSV.dial CBV @22:05:23.97 CTV.dial :intake @22:05:24.85 SSV.dial CBV @22:05:24.90 CBV.dial SSV @22:05:24.98 Allowing 40s for 7.0ml of remaining 7.0ml @22:05:25.00 Intake.select :open @22:05:27.27 Intake @31.3psia, vacuum from 20.0 to 24.0psi @22:05:27.35 SS.reconfigure SSinhale.with maxSpeed: 150 @22:05:27.45 SS.seek 7ml @22:05:33.07 Stroke completed -- waiting for pressure to equalize ... @22:05:47.61 Intake.select :close @22:05:47.85 SSV.dial :exhaust @22:05:48.70 Exhaust.select :open @22:05:48.88 SS.reconfigure SSexhale @22:05:48.97 SS.seek 0ml @22:05:52.80 Exhaust.select :close @22:05:52.96 Sampling 1500.0ml @22:05:52.99 Intake.select :close @22:05:53.01 Exhaust.select :close @22:05:53.04 SS.reconfigure SSconfig @22:05:53.12 SS.hold @22:05:53.57 CTV.dial :intake @22:05:53.62 SSV.dial CBV @22:05:53.67 CBV.dial SSV @22:05:54.59 Allowing 130s for 25.0ml of remaining 1500.0ml @22:05:54.62 Intake.select :open @22:05:56.91 Intake @31.4psia, vacuum from 20.0 to 24.0psi @22:05:56.95 SS.reconfigure SSinhale.with maxSpeed: 150 @22:05:57.04 SS.seek 25ml @22:06:00.68 Vacuum too high -- waiting up to 40s @22:06:03.26 Intake @30.6psia, vacuum from 20.0 to 24.0psi @22:06:03.28 SS.reconfigure SSinhale.with maxSpeed: 135 @22:06:03.33 SS.seek 25ml @22:06:06.38 Vacuum too high -- waiting up to 40s @22:06:09.70 Intake @30.6psia, vacuum from 20.0 to 24.0psi @22:06:09.72 SS.reconfigure SSinhale.with maxSpeed: 122 @22:06:09.77 SS.seek 25ml @22:06:12.56 Vacuum too high -- waiting up to 40s @22:06:16.00 Intake @30.7psia, vacuum from 20.0 to 24.0psi @22:06:16.03 SS.reconfigure SSinhale.with maxSpeed: 110 @22:06:16.07 SS.seek 25ml @22:06:18.63 Vacuum too high -- waiting up to 40s @22:06:21.83 Intake @30.7psia, vacuum from 20.0 to 24.0psi @22:06:21.85 SS.reconfigure SSinhale.with maxSpeed: 99 @22:06:21.90 SS.seek 25ml @22:06:24.59 Vacuum too high -- waiting up to 40s @22:06:28.11 Intake @30.8psia, vacuum from 20.0 to 24.0psi @22:06:28.13 SS.reconfigure SSinhale.with maxSpeed: 89 @22:06:28.18 SS.seek 25ml @22:06:31.27 Vacuum too high -- waiting up to 40s @22:06:34.60 Intake @30.8psia, vacuum from 20.0 to 24.0psi @22:06:34.62 SS.reconfigure SSinhale.with maxSpeed: 80 @22:06:34.67 SS.seek 25ml @22:06:37.31 Vacuum too high -- waiting up to 40s @22:06:40.75 Intake @30.8psia, vacuum from 20.0 to 24.0psi @22:06:40.77 SS.reconfigure SSinhale.with maxSpeed: 72 @22:06:40.82 SS.seek 25ml @22:06:43.88 Vacuum too high -- waiting up to 40s @22:06:47.22 Intake @30.9psia, vacuum from 20.0 to 24.0psi @22:06:47.24 SS.reconfigure SSinhale.with maxSpeed: 65 @22:06:47.29 SS.seek 25ml @22:06:49.02 Stroke completed -- waiting for pressure to equalize ... @22:06:54.70 Intake.select :close @22:06:54.86 SSV.dial :exhaust @22:06:55.68 Exhaust.select :open @22:06:55.80 SS.reconfigure SSexhale @22:06:55.84 SS.seek 0ml @22:07:04.89 Exhaust.select :close @22:07:05.01 SSV.dial CBV @22:07:05.03 CBV.dial SSV @22:07:05.87 Allowing 130s for 25.0ml of remaining 1475.0ml @22:07:05.88 Intake.select :open @22:07:08.05 Intake @31.3psia, vacuum from 20.0 to 24.0psi @22:07:08.07 SS.reconfigure SSinhale.with maxSpeed: 65 @22:07:08.12 SS.seek 25ml @22:07:12.50 Vacuum too high -- waiting up to 40s @22:07:14.83 Intake @30.9psia, vacuum from 20.0 to 24.0psi @22:07:14.85 SS.reconfigure SSinhale.with maxSpeed: 59 @22:07:14.90 SS.seek 25ml @22:07:19.99 Vacuum too high -- waiting up to 40s @22:07:22.69 Intake @31.0psia, vacuum from 20.0 to 24.0psi @22:07:22.71 SS.reconfigure SSinhale.with maxSpeed: 53 @22:07:22.77 SS.seek 25ml @22:07:27.03 Vacuum too high -- waiting up to 40s @22:07:29.61 Intake @30.9psia, vacuum from 20.0 to 24.0psi @22:07:29.63 SS.reconfigure SSinhale.with maxSpeed: 48 @22:07:29.68 SS.seek 25ml @22:07:34.60 Vacuum too high -- waiting up to 40s @22:07:37.17 Intake @31.0psia, vacuum from 20.0 to 24.0psi @22:07:37.19 SS.reconfigure SSinhale.with maxSpeed: 43 @22:07:37.24 SS.seek 25ml @22:07:42.85 Vacuum too high -- waiting up to 40s @22:07:45.42 Intake @30.9psia, vacuum from 20.0 to 24.0psi @22:07:45.44 SS.reconfigure SSinhale.with maxSpeed: 39 @22:07:45.49 SS.seek 25ml @22:07:52.30 Vacuum too high -- waiting up to 40s @22:07:54.88 Intake @31.0psia, vacuum from 20.0 to 24.0psi @22:07:54.90 SS.reconfigure SSinhale.with maxSpeed: 35 @22:07:54.95 SS.seek 25ml @22:08:03.71 Vacuum too high -- waiting up to 40s @22:08:06.42 SS.reconfigure SSinhale.with maxSpeed: 32 @22:08:06.48 SS.seek 25ml @22:08:12.36 Stroke completed -- waiting for pressure to equalize ... @22:08:19.37 Intake.select :close @22:08:19.53 SSV.dial :exhaust @22:08:20.36 Exhaust.select :open @22:08:20.48 SS.reconfigure SSexhale @22:08:20.53 SS.seek 0ml @22:08:29.45 Exhaust.select :close @22:08:29.57 SSV.dial CBV @22:08:29.59 CBV.dial SSV @22:08:30.43 Allowing 130s for 25.0ml of remaining 1450.0ml @22:08:30.44 Intake.select :open @22:08:32.63 Intake @31.3psia, vacuum from 20.0 to 24.0psi @22:08:32.65 SS.reconfigure SSinhale.with maxSpeed: 32 @22:08:32.70 SS.seek 25ml @22:08:50.25 Vacuum too high -- waiting up to 40s @22:08:52.57 Intake @30.9psia, vacuum from 20.0 to 24.0psi @22:08:52.59 SS.reconfigure SSinhale.with maxSpeed: 29 @22:08:52.64 SS.seek 25ml @22:09:20.00 Vacuum too high -- waiting up to 40s @22:09:22.83 Intake @31.0psia, vacuum from 20.0 to 24.0psi @22:09:22.85 SS.reconfigure SSinhale.with maxSpeed: 26 @22:09:22.90 SS.seek 25ml @22:09:39.69 Stroke completed -- waiting for pressure to equalize ... @22:09:47.27 Intake.select :close @22:09:47.43 SSV.dial :exhaust @22:09:48.25 Exhaust.select :open @22:09:48.37 SS.reconfigure SSexhale @22:09:48.42 SS.seek 0ml @22:09:57.40 Exhaust.select :close @22:09:57.53 SSV.dial CBV @22:09:57.55 CBV.dial SSV @22:09:58.39 Allowing 130s for 25.0ml of remaining 1425.0ml @22:09:58.40 Intake.select :open @22:10:00.59 Intake @31.4psia, vacuum from 20.0 to 24.0psi @22:10:00.61 SS.reconfigure SSinhale.with maxSpeed: 26 @22:10:00.65 SS.seek 25ml @22:11:08.49 Stroke completed -- waiting for pressure to equalize ... @22:11:17.32 Intake.select :close @22:11:17.48 SSV.dial :exhaust @22:11:18.31 Exhaust.select :open @22:11:18.43 SS.reconfigure SSexhale @22:11:18.48 SS.seek 0ml @22:11:27.65 Exhaust.select :close @22:11:27.77 SSV.dial CBV @22:11:27.79 CBV.dial SSV @22:11:28.61 Allowing 130s for 25.0ml of remaining 1400.0ml @22:11:28.62 Intake.select :open @22:11:30.79 Intake @31.4psia, vacuum from 20.0 to 24.0psi @22:11:30.81 SS.reconfigure SSinhale.with maxSpeed: 26 @22:11:30.86 SS.seek 25ml @22:12:27.40 Vacuum too high -- waiting up to 40s @22:12:29.98 Intake @31.0psia, vacuum from 20.0 to 24.0psi @22:12:30.00 SS.reconfigure SSinhale.with maxSpeed: 23 @22:12:30.05 SS.seek 25ml @22:12:42.82 Stroke completed -- waiting for pressure to equalize ... @22:12:50.35 Intake.select :close @22:12:50.51 SSV.dial :exhaust @22:12:51.34 Exhaust.select :open @22:12:51.46 SS.reconfigure SSexhale @22:12:51.51 SS.seek 0ml @22:13:00.51 Exhaust.select :close @22:13:00.63 SSV.dial CBV @22:13:00.65 CBV.dial SSV @22:13:01.48 Allowing 130s for 25.0ml of remaining 1375.0ml @22:13:01.49 Intake.select :open @22:13:03.68 Intake @31.4psia, vacuum from 20.0 to 24.0psi @22:13:03.70 SS.reconfigure SSinhale.with maxSpeed: 23 @22:13:03.75 SS.seek 25ml @22:14:20.11 Stroke completed -- waiting for pressure to equalize ... @22:14:29.57 Intake.select :close @22:14:29.73 SSV.dial :exhaust @22:14:30.55 Exhaust.select :open @22:14:30.67 SS.reconfigure SSexhale @22:14:30.71 SS.seek 0ml @22:14:40.05 Exhaust.select :close @22:14:40.17 SSV.dial CBV @22:14:40.19 CBV.dial SSV @22:14:41.03 Allowing 130s for 25.0ml of remaining 1350.0ml @22:14:41.04 Intake.select :open @22:14:43.21 Intake @31.4psia, vacuum from 20.0 to 24.0psi @22:14:43.23 SS.reconfigure SSinhale.with maxSpeed: 23 @22:14:43.28 SS.seek 25ml @22:15:59.88 Stroke completed -- waiting for pressure to equalize ... @22:16:10.54 Intake.select :close @22:16:10.70 SSV.dial :exhaust @22:16:11.51 Exhaust.select :open @22:16:11.63 SS.reconfigure SSexhale @22:16:11.67 SS.seek 0ml @22:16:20.83 Exhaust.select :close @22:16:21.55 SSV.dial CBV @22:16:21.57 CBV.dial SSV @22:16:22.41 Allowing 130s for 25.0ml of remaining 1325.0ml @22:16:22.42 Intake.select :open @22:16:24.61 Intake @31.4psia, vacuum from 20.0 to 24.0psi @22:16:24.63 SS.reconfigure SSinhale.with maxSpeed: 23 @22:16:24.68 SS.seek 25ml @22:17:22.72 Vacuum too high -- waiting up to 40s @22:17:25.84 Intake @30.9psia, vacuum from 20.0 to 24.0psi @22:17:25.86 SS.reconfigure SSinhale.with maxSpeed: 21 @22:17:25.91 SS.seek 25ml @22:17:46.44 Stroke completed -- waiting for pressure to equalize ... @22:17:56.47 Intake.select :close @22:17:56.63 SSV.dial :exhaust @22:17:57.45 Exhaust.select :open @22:17:57.57 SS.reconfigure SSexhale @22:17:57.62 SS.seek 0ml @22:18:06.86 Exhaust.select :close @22:18:06.98 SSV.dial CBV @22:18:07.00 CBV.dial SSV @22:18:07.84 Allowing 130s for 25.0ml of remaining 1300.0ml @22:18:07.85 Intake.select :open @22:18:10.03 Intake @31.4psia, vacuum from 20.0 to 24.0psi @22:18:10.05 SS.reconfigure SSinhale.with maxSpeed: 21 @22:18:10.10 SS.seek 25ml @22:19:15.15 Vacuum too high -- waiting up to 40s @22:19:19.09 Intake @31.0psia, vacuum from 20.0 to 24.0psi @22:19:19.11 SS.reconfigure SSinhale.with maxSpeed: 19 @22:19:19.16 SS.seek 25ml @22:19:39.82 Stroke completed -- waiting for pressure to equalize ... @22:19:51.20 Intake.select :close @22:19:51.36 SSV.dial :exhaust @22:19:52.19 Exhaust.select :open @22:19:52.31 SS.reconfigure SSexhale @22:19:52.36 SS.seek 0ml @22:20:01.86 Exhaust.select :close @22:20:01.98 SSV.dial CBV @22:20:02.00 CBV.dial SSV @22:20:02.84 Allowing 130s for 25.0ml of remaining 1275.0ml @22:20:02.85 Intake.select :open @22:20:05.02 Intake @31.4psia, vacuum from 20.0 to 24.0psi @22:20:05.04 SS.reconfigure SSinhale.with maxSpeed: 19 @22:20:05.10 SS.seek 25ml @22:21:20.60 Vacuum too high -- waiting up to 40s @22:21:23.95 Intake @31.1psia, vacuum from 20.0 to 24.0psi @22:21:23.97 SS.reconfigure SSinhale.with maxSpeed: 17 @22:21:24.02 SS.seek 25ml @22:21:43.42 Stroke completed -- waiting for pressure to equalize ... @22:21:56.03 Intake.select :close @22:21:56.19 SSV.dial :exhaust @22:21:57.02 Exhaust.select :open @22:21:57.14 SS.reconfigure SSexhale @22:21:57.18 SS.seek 0ml @22:22:06.13 Exhaust.select :close @22:22:06.25 SSV.dial CBV @22:22:06.27 CBV.dial SSV @22:22:07.11 Allowing 130s for 25.0ml of remaining 1250.0ml @22:22:07.12 Intake.select :open @22:22:09.30 Intake @31.4psia, vacuum from 20.0 to 24.0psi @22:22:09.32 SS.reconfigure SSinhale.with maxSpeed: 17 @22:22:09.37 SS.seek 25ml @22:23:49.72 Vacuum too high -- waiting up to 40s @22:23:53.79 Intake @31.0psia, vacuum from 20.0 to 24.0psi @22:23:53.81 SS.reconfigure SSinhale.with maxSpeed: 15 @22:23:53.86 SS.seek 25ml @22:23:57.96 Stroke completed -- waiting for pressure to equalize ... @22:24:10.47 Intake.select :close @22:24:10.63 SSV.dial :exhaust @22:24:11.45 Exhaust.select :open @22:24:11.57 SS.reconfigure SSexhale @22:24:11.61 SS.seek 0ml @22:24:21.49 Exhaust.select :close @22:24:21.61 SSV.dial CBV @22:24:21.63 CBV.dial SSV @22:24:22.47 Allowing 130s for 25.0ml of remaining 1225.0ml @22:24:22.48 Intake.select :open @22:24:24.66 Intake @31.4psia, vacuum from 20.0 to 24.0psi @22:24:24.69 SS.reconfigure SSinhale.with maxSpeed: 15 @22:24:24.73 SS.seek 25ml @22:26:11.01 Vacuum too high -- waiting up to 40s @22:26:15.60 Intake @31.0psia, vacuum from 20.0 to 24.0psi @22:26:15.62 SS.reconfigure SSinhale.with maxSpeed: 14 @22:26:15.66 SS.seek 25ml @22:26:27.88 Stroke completed -- waiting for pressure to equalize ... @22:26:32.49 SS.reconfigure SSconfig @22:26:32.54 SS.hold @22:26:32.67 Intake.select :close @22:26:32.83 ShallowSampler::Clogged in WCR -- progress too slow after 300.0ml @22:26:32.85 SSV.dial :exhaust @22:26:33.67 Exhaust.select :open @22:26:33.79 SS.reconfigure SSexhale @22:26:33.84 SS.seek 0ml @22:26:43.20 Exhaust.select :close @22:26:43.31 Sampled 300.0ml @22:26:43.33 Drying puck before fixing for 4:00 @22:26:43.34 CBV.dial :waste2b @22:26:44.14 delay 1:00 @22:27:44.17 CSV.dial :air @22:27:44.95 CS.seek 10ml @22:28:08.69 Pumping wcr puck from tube 3 up to 55psi... @22:28:08.70 CBV.dial SSV @22:28:10.11 CBV.dial between CSV and waste2b @22:28:11.15 CSV.dial between air and CTV @22:28:11.49 CS.seek 7.186ml @22:28:18.91 CTV.dial CSV,avoiding: :air @22:28:18.94 CSV.dial CTV @22:28:19.79 CS.reconfigure CSpressurize @22:28:19.84 CS.seek 0ml @22:28:37.43 CBV.dial :waste2b @22:28:37.79 CS.reconfigure CSconfig @22:28:38.45 CS.seek :empty @22:28:40.69 delay 1:00 @22:29:40.72 CTV.dial :air,avoiding: :intake @22:29:42.12 Fix #1 of 2 (with rnal) for 2:00 @22:29:42.14 CSV.dial CSR @22:29:42.66 Pulling 2ml of CSR.rnal @22:29:42.68 CSR.select :rnal @22:29:42.80 CS.seek 2ml @22:29:48.98 delay 5 seconds @22:29:54.01 CSR.select :air @22:29:54.14 CS.reconfigure CSfast @22:29:54.19 CS.seek 10ml @22:30:13.20 Pumping wcr puck from tube 3 up to 55psi... @22:30:13.22 CBV.dial SSV @22:30:14.59 CBV.dial between CSV and waste2b @22:30:15.61 CSV.dial between air and CTV @22:30:16.27 CS.seek 7.226ml @22:30:23.58 CTV.dial CSV,avoiding: :air @22:30:23.60 CSV.dial CTV @22:30:25.08 CS.reconfigure CSpressurize @22:30:25.13 CS.seek 0ml @22:30:43.37 delay 2:00 @22:32:43.41 CBV.dial :waste2b @22:32:43.76 CS.reconfigure CSconfig @22:32:43.81 CS.seek :empty @22:32:45.48 delay 1:00 @22:33:45.51 CTV.dial :air,avoiding: :intake @22:33:46.89 Fix #2 of 2 (with rnal) for 2:00 @22:33:46.91 CSV.dial CSR @22:33:47.43 Pulling 2ml of CSR.rnal @22:33:47.45 CSR.select :rnal @22:33:47.57 CS.seek 2ml @22:33:53.67 delay 5 seconds @22:33:58.70 CSR.select :air @22:33:58.83 CS.reconfigure CSfast @22:33:58.88 CS.seek 10ml @22:34:17.86 Pumping wcr puck from tube 3 up to 55psi... @22:34:17.88 CBV.dial SSV @22:34:19.24 CBV.dial between CSV and waste2b @22:34:20.27 CSV.dial between air and CTV @22:34:20.91 CS.seek 7.199ml @22:34:28.42 CTV.dial CSV,avoiding: :air @22:34:28.45 CSV.dial CTV @22:34:29.92 CS.reconfigure CSpressurize @22:34:29.98 CS.seek 0ml @22:34:48.07 delay 2:00 @22:36:48.10 CBV.dial :waste2b @22:36:48.45 CS.reconfigure CSconfig @22:36:48.50 CS.seek :empty @22:36:50.20 delay 1:00 @22:37:50.23 CTV.dial :air,avoiding: :intake @22:37:51.68 Storing wcr puck in Collection Clamp to tube 2 @22:37:51.87 SC.reconfigure SCtight @22:37:51.92 SC.seek 2 @22:37:54.10 SC.reconfigure SCloose @22:37:54.90 Elbow.reconfigure TightElbow @22:37:54.96 Elbow.seek 2 @22:37:56.90 Forearm.reconfigure TightForearm @22:37:56.95 Forearm.seek 2 + 700 counts @22:37:58.00 SE.reconfigure SEpick @22:37:58.08 SE.seek beamBreak @22:38:01.03 SE.configure SEconfig @22:38:01.20 Elbow.reconfigure LimpElbow @22:38:01.25 Forearm.reconfigure LooseForearm @22:38:01.30 Forearm.seek 2 @22:38:01.59 Elbow.hold @22:38:01.67 Elbow.reconfigure TightElbow @22:38:01.79 SE.seek 0.07 Pucks below Top Plate @22:38:02.25 Forearm.force -20 @22:38:02.30 Forearm.seek 2 + 337 counts @22:38:02.63 Forearm.reconfigure TightForearm @22:38:02.68 Forearm.seek 2 + 700 counts @22:38:02.91 SE.seek 1.07 Pucks below Top Plate @22:38:03.50 Forearm.reconfigure LooseForearm @22:38:03.55 Forearm.seek :retract @22:38:04.49 Elbow.seek CC @22:38:06.21 Elbow.reconfigure LooseElbow @22:38:06.32 Forearm.seek CC @22:38:07.38 Hand.close @22:38:08.02 CC.seek :open @22:38:12.53 Forearm.seek :retract @22:38:13.63 Elbow.reconfigure TightElbow @22:38:13.68 Elbow.seek 2 @22:38:15.65 Elbow.reconfigure LimpElbow @22:38:15.71 Forearm.seek 2 @22:38:16.70 Elbow.hold @22:38:16.78 Elbow.reconfigure TightElbow @22:38:16.82 Hand.open @22:38:17.54 Forearm.force -20 @22:38:17.59 Forearm.seek 2 + 347 counts @22:38:17.91 Forearm.reconfigure TightForearm @22:38:17.96 Forearm.seek 2 + 700 counts @22:38:18.39 SE.seek :down @22:38:19.54 Forearm.reconfigure LooseForearm @22:38:19.59 Forearm.seek :retract @22:38:21.10 SE.coast @22:38:21.14 SC.coast @22:38:21.18 Starting finalPurge @22:38:21.20 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:38:21.27 Hand.open @22:38:22.29 Elbow.seek FlushPuck::Garage @22:38:24.34 Elbow.reconfigure LooseElbow @22:38:24.45 Forearm.seek :garage @22:38:25.46 Hand.close @22:38:26.16 Forearm.seek :retract @22:38:27.17 Elbow.reconfigure TightElbow @22:38:27.22 Elbow.seek CC @22:38:30.12 Elbow.reconfigure LooseElbow @22:38:30.23 Forearm.reconfigure TightForearm @22:38:30.28 Forearm.seek CC @22:38:31.64 CC.seek :closed @22:38:35.92 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.97 @22:38:35.99 Hand.open @22:38:36.72 Forearm.reconfigure LooseForearm @22:38:36.77 Forearm.seek :retract @22:38:37.79 Flushing the Collection Valve::Manifold @22:38:37.93 CSV.dial CSR @22:38:38.45 Pulling 5ml of CSR.flush @22:38:38.46 CSR.select :flush @22:38:38.59 CS.seek 5ml @22:38:52.43 delay 5 seconds @22:38:57.46 CSR.select :air @22:38:57.63 CS.reconfigure CSfast @22:38:57.68 CS.seek 7.5ml @22:39:04.43 CSV.dial :waste @22:39:05.78 CS.reconfigure CSconfig @22:39:05.83 CS.seek 0ml @22:39:25.99 Flushing lower manifold's waste1 port @22:39:26.12 CSV.dial :flush @22:39:26.60 CS.reconfigure CSfast @22:39:26.65 CS.seek 5ml @22:39:38.93 delay 5 seconds @22:39:43.96 CSV.dial :air @22:39:44.50 CS.seek 7ml @22:39:50.08 CSV.dial CTV @22:39:50.12
CTV.dial CSV @22:39:51.58 CBV.dial :waste1 @22:39:52.39 CS.seek 0ml @22:40:09.08 Flushing lower manifold's waste2b port @22:40:09.21 CSV.dial :flush @22:40:10.00 CS.seek 5ml @22:40:22.40 delay 5 seconds @22:40:27.43 CSV.dial :air @22:40:27.98 CS.seek 7ml @22:40:33.49 CSV.dial CTV @22:40:33.51
CTV.dial CSV @22:40:34.00 CBV.dial :waste2b @22:40:34.81 CS.seek 0ml @22:40:51.58 Flushing upper manifold @22:40:51.71 CSV.dial :flush @22:40:52.50 CS.seek 5ml @22:41:05.40 delay 5 seconds @22:41:10.43 CSV.dial :air @22:41:10.98 CS.seek 7ml @22:41:16.62 CSV.dial CBV @22:41:16.66
CBV.dial CSV @22:41:17.79 CTV.dial :air @22:41:19.20 CS.seek 0ml @22:41:35.99 delay 7 seconds @22:41:43.03 CS.seek 3ml @22:41:50.90 CSV.dial :waste @22:41:51.39 CS.seek 0ml @22:41:59.49 CSV.dial :flush @22:41:59.97 CS.seek 3ml @22:42:07.74 delay 5 seconds @22:42:12.77 CSV.dial :air @22:42:13.31 CS.seek 5ml @22:42:18.90 CSV.dial :air @22:42:18.92 CS.seek 0ml @22:42:31.21 delay 7 seconds @22:42:38.25 CS.seek 3ml @22:42:46.18 CSV.dial :waste @22:42:46.95 CS.seek 0ml @22:42:54.93 Disinfecting external Intake with mfbkill @22:42:55.06 CSV.dial CSR @22:42:56.47 Pulling 8ml of CSR.mfbkill @22:42:56.48 CSR.select :mfbkill @22:42:56.61 CS.seek 8ml @22:43:15.65 delay 5 seconds @22:43:20.68 CSR.select :air @22:43:20.80 CS.seek 10ml @22:43:26.38 CSV.dial CBV @22:43:26.41
CBV.dial CSV @22:43:27.49 CTV.dial :intake @22:43:28.27 Intake.select :open @22:43:28.39 CS.seek 2ml @22:43:48.71 Intake.select :close @22:43:48.82 CTV.dial :air @22:43:49.62 CS.reconfigure CSconfig @22:43:49.67 CS.seek 0ml @22:43:55.71 Flushing lower manifold's waste1 port @22:43:55.72 CSV.dial CSR @22:43:56.85 Pulling 4ml of CSR.mfbkill @22:43:56.86 CSR.select :mfbkill @22:43:56.99 CS.seek 4ml @22:44:08.21 delay 5 seconds @22:44:13.24 CSR.select :air @22:44:13.37 CS.reconfigure CSfast @22:44:13.41 CS.seek 10ml @22:44:27.90 CSV.dial CTV @22:44:27.93
CTV.dial CSV @22:44:29.40 CBV.dial :waste1 @22:44:29.89 CS.seek 0ml @22:44:53.37 Flushing lower manifold's waste2b port @22:44:53.38 CSV.dial CSR @22:44:53.91 Pulling 4ml of CSR.mfbkill @22:44:53.92 CSR.select :mfbkill @22:44:54.04 CS.reconfigure CSconfig @22:44:54.08 CS.seek 4ml @22:45:05.31 delay 5 seconds @22:45:10.34 CSR.select :air @22:45:10.47 CS.reconfigure CSfast @22:45:10.51 CS.seek 10ml @22:45:25.09 CSV.dial CTV @22:45:25.12
CTV.dial CSV @22:45:25.60 CBV.dial :waste2b @22:45:26.41 CS.seek 0ml @22:45:50.09 Killing upper manifold @22:45:50.10 CSV.dial CSR @22:45:50.63 Pulling 3.999ml of CSR.mfbkill @22:45:50.64 CSR.select :mfbkill @22:45:50.76 CS.reconfigure CSconfig @22:45:50.80 CS.seek 4ml @22:46:01.96 delay 5 seconds @22:46:06.99 CSR.select :air @22:46:07.11 CS.reconfigure CSfast @22:46:07.16 CS.seek 10ml @22:46:21.68 CSV.dial CBV @22:46:21.71
CBV.dial CSV @22:46:23.45 CTV.dial :air @22:46:24.83 CS.seek 0ml @22:46:48.34 delay 7 seconds @22:46:55.38 CS.seek 3ml @22:47:03.28 CSV.dial :waste @22:47:03.76 CS.seek 0ml @22:47:11.62 Flushing upper manifold @22:47:11.76 CSV.dial :flush @22:47:12.23 CS.seek 5ml @22:47:24.59 delay 5 seconds @22:47:29.62 CSV.dial :air @22:47:30.29 CS.seek 7.001ml @22:47:35.97 CSV.dial CBV @22:47:36.00
CBV.dial CSV @22:47:37.06 CTV.dial :air @22:47:37.08 CS.seek 0ml @22:47:53.84 delay 7 seconds @22:48:00.88 CS.seek 3ml @22:48:08.75 CSV.dial :waste @22:48:09.23 CS.seek 0ml @22:48:17.21 CSV.dial :flush @22:48:17.81 CS.seek 3ml @22:48:25.56 delay 5 seconds @22:48:30.59 CSV.dial :air @22:48:31.27 CS.seek 5ml @22:48:36.81 CSV.dial :air @22:48:36.83 CS.seek 0ml @22:48:49.12 delay 7 seconds @22:48:56.16 CS.seek 3ml @22:49:04.00 CSV.dial :waste @22:49:04.76 CS.seek 0ml @22:49:12.59 Flushing lower manifold's waste1 port @22:49:12.73 CSV.dial :flush @22:49:13.21 CS.seek 5ml @22:49:25.44 delay 5 seconds @22:49:30.47 CSV.dial :air @22:49:31.13 CS.seek 7ml @22:49:36.78 CSV.dial CTV @22:49:36.82
CTV.dial CSV @22:49:38.28 CBV.dial :waste1 @22:49:38.78 CS.seek 0ml @22:49:55.50 Flushing lower manifold's waste2b port @22:49:55.64 CSV.dial :flush @22:49:56.42 CS.seek 5ml @22:50:08.78 delay 5 seconds @22:50:13.80 CSV.dial :air @22:50:14.35 CS.seek 7ml @22:50:20.00 CSV.dial CTV @22:50:20.04
CTV.dial CSV @22:50:20.50 CBV.dial :waste2b @22:50:21.32 CS.seek 0ml @22:50:37.97 Disinfecting external Intake with kill @22:50:38.10 CSV.dial CSR @22:50:38.62 Pulling 8ml of CSR.kill @22:50:38.63 CSR.select :kill @22:50:38.76 CS.seek 8ml @22:50:57.75 delay 5 seconds @22:51:02.78 CSR.select :air @22:51:02.91 CS.seek 10ml @22:51:08.47 CSV.dial CBV @22:51:08.49
CBV.dial CSV @22:51:09.63 CTV.dial :intake @22:51:10.41 Intake.select :open @22:51:10.53 CS.seek 2ml @22:51:30.63 Intake.select :close @22:51:30.74 CTV.dial :air @22:51:31.53 CS.reconfigure CSconfig @22:51:31.58 CS.seek 0ml @22:51:37.69 CSV.dial CSR @22:51:39.41 Pulling 5ml of CSR.flush @22:51:39.42 CSR.select :flush @22:51:39.54 CS.seek 5ml @22:51:53.32 delay 5 seconds @22:51:58.35 CSR.select :air @22:51:58.48 CS.seek 10ml @22:52:12.32 CSV.dial CBV @22:52:13.40 CS.seek 0ml @22:52:40.25 Garaging FlushPuck in Collection Clamp @22:52:40.33 Elbow.reconfigure TightElbow @22:52:40.38 Elbow.seek CC @22:52:40.61 Elbow.reconfigure LooseElbow @22:52:40.72 Forearm.seek CC @22:52:41.72 Hand.close @22:52:42.30 CC.seek :open @22:52:46.67 Forearm.seek :retract @22:52:47.69 Elbow.reconfigure TightElbow @22:52:47.74 Elbow.seek :garage @22:52:51.00 Elbow.reconfigure LooseElbow @22:52:51.11 Forearm.reconfigure TightForearm @22:52:51.15 Forearm.seek :garage @22:52:52.31 Hand.open @22:52:53.00 Forearm.reconfigure LooseForearm @22:52:53.05 Forearm.seek :retract @22:52:54.08 Starting initialPurge @22:52:54.09 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:52:54.17 Hand.open @22:52:54.50 Elbow.reconfigure TightElbow @22:52:54.54 Elbow.seek FlushPuck::Garage @22:52:54.67 Elbow.reconfigure LooseElbow @22:52:54.77 Forearm.seek :garage @22:52:55.78 Hand.close @22:52:56.56 Forearm.seek :retract @22:52:57.61 Elbow.reconfigure TightElbow @22:52:57.66 Elbow.seek CC @22:53:00.63 Elbow.reconfigure LooseElbow @22:53:00.73 Forearm.reconfigure TightForearm @22:53:00.78 Forearm.seek CC @22:53:02.08 CC.seek :closed @22:53:06.35 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.95 @22:53:06.42 Hand.open @22:53:07.12 Forearm.reconfigure LooseForearm @22:53:07.16 Forearm.seek :retract @22:53:08.20 Flushing the Collection Valve::Manifold @22:53:08.33 CSV.dial CSR @22:53:09.47 Pulling 5ml of CSR.flush @22:53:09.48 CSR.select :flush @22:53:09.60 CS.seek 5ml @22:53:23.35 delay 5 seconds @22:53:28.38 CSR.select :air @22:53:28.54 CS.reconfigure CSfast @22:53:28.59 CS.seek 7.5ml @22:53:35.22 CSV.dial :waste @22:53:36.57 CS.reconfigure CSconfig @22:53:36.62 CS.seek 0ml @22:53:56.79 Purging external Intake @22:53:56.92 CSV.dial :flush @22:53:58.00 CS.reconfigure CSfast @22:53:58.06 CS.seek 10ml @22:54:21.32 delay 5 seconds @22:54:26.35 CSV.dial CBV @22:54:26.39
CBV.dial CSV @22:54:27.19 CTV.dial :intake @22:54:27.98 Intake.select :open @22:54:28.10 CS.seek 0ml @22:54:53.19 Intake.select :close @22:54:53.30 CTV.dial :air @22:54:54.08 Flushing upper manifold @22:54:54.21 CSV.dial :flush @22:54:55.00 CS.seek 5ml @22:55:07.16 delay 5 seconds @22:55:12.18 CSV.dial :air @22:55:12.84 CS.seek 7ml @22:55:18.35 CSV.dial CBV @22:55:18.37
CBV.dial CSV @22:55:19.46 CTV.dial :air @22:55:19.48 CS.seek 0ml @22:55:36.29 delay 7 seconds @22:55:43.33 CS.seek 3ml @22:55:51.20 CSV.dial :waste @22:55:51.69 CS.seek 0ml @22:55:59.60 CSV.dial :flush @22:56:00.06 CS.seek 3ml @22:56:07.91 delay 5 seconds @22:56:12.94 CSV.dial :air @22:56:13.48 CS.seek 5.001ml @22:56:19.13 CSV.dial :air @22:56:19.15 CS.seek 0ml @22:56:31.41 delay 7 seconds @22:56:38.45 CS.seek 3ml @22:56:46.20 CSV.dial :waste @22:56:46.99 CS.seek 0ml @22:56:55.10 Garaging FlushPuck in Collection Clamp @22:56:55.18 Elbow.reconfigure TightElbow @22:56:55.23 Elbow.seek CC @22:56:55.45 Elbow.reconfigure LooseElbow @22:56:55.56 Forearm.seek CC @22:56:56.56 Hand.close @22:56:57.18 CC.seek :open @22:57:01.55 Forearm.seek :retract @22:57:02.54 Elbow.reconfigure TightElbow @22:57:02.59 Elbow.seek :garage @22:57:05.87 Elbow.reconfigure LooseElbow @22:57:05.97 Forearm.reconfigure TightForearm @22:57:06.02 Forearm.seek :garage @22:57:07.16 Hand.open @22:57:07.88 Forearm.reconfigure LooseForearm @22:57:07.92 Forearm.seek :retract @22:57:08.96 Completed [wcrphase] begun 22:00:25 @22:57:08.97 Preparing to sleep until 04:00 21-may-20 @22:57:09.03 SS.coast @22:57:09.06
Asleep til 04:00 21-may-20 @22:57:09.13 CC.coast @22:57:09.17
Continuing at 04:00CEST21-May-20 @22:57:09.23 CS.coast @22:57:09.33 PC.coast @22:57:09.38 GoodNews.email "Then, in tube 3: wcrphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:141\n{1 => 22, 2 => 2, 3 => 20}\nCan@22:57:09, 9.8C, 44% humidity, 14.4psia, 13.549V, 0.474A, 0.468A avg, 6.43W",Subject: "Asleep until 04:00CEST21-May-20" @22:57:09.46 Forearm.coast @22:57:09.51 Elbow.coast @22:57:09.95 Gate.power :core,:OFF @22:57:09.97 Gate.power :raw,:OFF @22:57:09.99 Shutdown @04:00:00.05CEST21-May-20
Powering core bus @04:00:05.12 Gate.power :raw,:ON @04:00:05.78 SPE.coast @04:00:05.85 SPE.configure SPEconfig @04:00:05.94 AS.configure ASconfig @04:00:06.04 SS.configure SSconfig @04:00:06.14 CC.configure CCconfig @04:00:06.24 CS.configure CSconfig @04:00:06.34 PC.configure PCconfig @04:00:06.44 PS.configure PSconfig @04:00:06.56 Forearm.configure LooseForearm @04:00:06.66 Elbow.configure LooseElbow @04:00:06.78 SC.configure SCloose @04:00:06.88 SE.configure SEconfig @04:00:06.93 CH.coast @04:00:06.99 CH.configure CHconfig @04:00:07.02 PH.coast @04:00:07.08 PH.configure PHconfig @04:00:07.12 PCR.off @04:00:07.16 PCR.pidtemp= 50,0.1,0,300,25,25 @04:00:07.20 PCR.caltemp= 2.8814,0.0372,0 @04:00:07.23 All dwarf controllers running firmware version 3.75 @04:00:07.34 Valve.reset @04:00:07.51 PCR.off @04:00:07.54 PCR.pidtemp= 50,0.1,0,300,25,25 @04:00:07.57 PCR.caltemp= 2.8814,0.0372,0 @04:00:07.79 PSV.dial :air @04:00:07.82 Intake.select :close @04:00:07.85 Exhaust.select :close @04:00:07.87 CTV.dial :air @04:00:08.01 CSV.dial :waste1 @04:00:08.06 PS.reconfigure PSconfig @04:00:08.20 PS.home.seek 0ml @04:00:08.23 SSV.dial :spit,avoiding: :exhaust @04:00:08.25
Forearm.reconfigure LooseForearm @04:00:08.37 Forearm.home.seek :retract @04:00:09.14 Hand.close @04:00:09.19
Elbow.jog 1500 @04:00:09.29 CS.reconfigure CSfast @04:00:09.33 SS.reconfigure SSconfig @04:00:09.43 CS.home.seek 0ml @04:00:09.45 SS.home.seek 0ml @04:00:09.87
Elbow.reconfigure LooseElbow @04:00:09.92 Elbow.home.seek :clear @04:00:13.35 SE.reconfigure SEconfig @04:00:13.39 SE.home.seek :down @04:00:13.99 SC.reconfigure SCsloppy @04:00:14.04 SC.home.seek :initial @04:00:17.35 PS.coast @04:00:18.03
Forearm.seek :retract @04:00:18.11 Elbow.seek :hammer @04:00:19.29 Forearm.reconfigure TightForearm @04:00:19.34 Forearm.seek 5 + 350 counts @04:00:20.38 Forearm.seek :retract @04:00:21.54 PC.reconfigure PCconfig @04:00:21.56
CC.reconfigure CCconfig @04:00:21.64 PC.home.seek :open @04:00:21.68
CC.home.seek :open @04:00:25.22 Phase [wcrphase] @04:00:25.24 GoodNews.email "At 04:00:25 began in tube 3: wcrphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:141\n{1 => 22, 2 => 2, 3 => 20}\nCan@04:00:25, 9.5C, 45% humidity, 14.4psia, 13.564V, 0.638A, 0.638A avg, 8.66W",Subject: "Started 20invasivedeployfinal phase wcrphase" @04:00:25.26CEST21-May-20
===> STARTED WCRSphase (phase #10) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @04:00:25.30 Starting initialPurge @04:00:25.32 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @04:00:25.39 Hand.open @04:00:26.20 Elbow.reconfigure TightElbow @04:00:26.25 Elbow.seek FlushPuck::Garage @04:00:28.41 Elbow.reconfigure LooseElbow @04:00:28.51 Forearm.reconfigure LooseForearm @04:00:28.56 Forearm.seek :garage @04:00:29.58 Hand.close @04:00:30.34 Forearm.seek :retract @04:00:31.41 Elbow.reconfigure TightElbow @04:00:31.49 Elbow.seek CC @04:00:34.50 Elbow.reconfigure LooseElbow @04:00:34.75 Forearm.reconfigure TightForearm @04:00:34.84 Forearm.seek CC @04:00:36.51 CC.seek :closed @04:00:41.07 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.05 @04:00:41.27 Hand.open @04:00:42.16 Forearm.reconfigure LooseForearm @04:00:42.25 Forearm.seek :retract @04:00:43.30 Flushing the Collection Valve::Manifold @04:00:43.59 CSV.dial CSR @04:00:45.13 Pulling 5ml of CSR.flush @04:00:45.16 CSR.select :flush @04:00:45.35 CS.reconfigure CSconfig @04:00:45.45 CS.seek 5ml @04:00:59.24 delay 5 seconds @04:01:04.29 CSR.select :air @04:01:04.54 CS.reconfigure CSfast @04:01:04.68 CS.seek 7.5ml @04:01:11.51 CSV.dial :waste @04:01:12.91 CS.reconfigure CSconfig @04:01:13.01 CS.seek 0ml @04:01:33.24 Purging external Intake @04:01:33.37 CSV.dial :flush @04:01:33.85 CS.reconfigure CSfast @04:01:33.90 CS.seek 10ml @04:01:57.68 delay 5 seconds @04:02:02.70 CSV.dial CBV @04:02:02.74
CBV.dial CSV @04:02:03.67 CTV.dial :intake @04:02:04.46 Intake.select :open @04:02:04.58 CS.seek 0ml @04:02:29.93 Intake.select :close @04:02:30.04 CTV.dial :air @04:02:30.84 Flushing upper manifold @04:02:30.97 CSV.dial :flush @04:02:31.74 CS.seek 5ml @04:02:44.12 delay 5 seconds @04:02:49.15 CSV.dial :air @04:02:50.27 CS.seek 7ml @04:02:55.87 CSV.dial CBV @04:02:55.89
CBV.dial CSV @04:02:56.98 CTV.dial :air @04:02:57.00 CS.seek 0ml @04:03:13.84 delay 7 seconds @04:03:20.88 CS.seek 3ml @04:03:28.81 CSV.dial :waste @04:03:29.29 CS.seek 0ml @04:03:37.46 CSV.dial :flush @04:03:37.93 CS.seek 3ml @04:03:45.84 delay 5 seconds @04:03:50.87 CSV.dial :air @04:03:51.54 CS.seek 5ml @04:03:57.22 CSV.dial :air @04:03:57.24 CS.seek 0ml @04:04:09.62 delay 7 seconds @04:04:16.66 CS.seek 3ml @04:04:24.53 CSV.dial :waste @04:04:25.29 CS.seek 0ml @04:04:33.15 Garaging FlushPuck in Collection Clamp @04:04:33.23 Elbow.reconfigure TightElbow @04:04:33.28 Elbow.seek CC @04:04:33.53 Elbow.reconfigure LooseElbow @04:04:33.64 Forearm.seek CC @04:04:34.61 Hand.close @04:04:35.21 CC.seek :open @04:04:39.53 Forearm.seek :retract @04:04:40.53 Elbow.reconfigure TightElbow @04:04:40.58 Elbow.seek :garage @04:04:43.73 Elbow.reconfigure LooseElbow @04:04:43.83 Forearm.reconfigure TightForearm @04:04:43.88 Forearm.seek :garage @04:04:45.02 Hand.open @04:04:45.65 Forearm.reconfigure LooseForearm @04:04:45.69 Forearm.seek :retract @04:04:46.74 Starting stand-alone bac WCR @04:04:46.75 bac wcr sampling at most 1500ml @04:04:46.78 BACfans.email "Started stand-alone bac WCR in tube 3 at 04:04:46CEST21-May-20\n{1 => 22, 2 => 2, 3 => 20}\nCan@04:00:25, 9.5C, 45% humidity, 14.4psia, 13.564V, 0.638A, 0.638A avg, 8.66W",Subject: "bac wcr sampling at most 1500ml" @04:04:46.81 Whole Cell Archive sampling 1500ml @04:04:46.85 Loading wcr puck from tube 3 into Collection Clamp @04:04:47.12 SC.reconfigure SCtight @04:04:47.17 SC.seek 3 @04:04:49.25 SC.reconfigure SCloose @04:04:49.86 Elbow.reconfigure TightElbow @04:04:49.95 Elbow.seek 3 @04:04:52.17 Forearm.reconfigure TightForearm @04:04:52.32 Forearm.seek 3 + 700 counts @04:04:53.49 SE.reconfigure SEpick @04:04:53.58 SE.seek beamBreak @04:04:55.76 SE.configure SEconfig @04:04:56.19 Elbow.seek 3 @04:04:56.68 Forearm.seek 3 + 300 counts @04:04:56.99 Forearm.reconfigure LooseForearm @04:04:57.09 Hand.close @04:04:57.89 SE.seek :down @04:04:58.42 Forearm.seek :retract @04:04:59.06 SE.coast @04:04:59.42 Elbow.seek CC @04:05:01.33 Elbow.reconfigure LooseElbow @04:05:01.61 Forearm.reconfigure TightForearm @04:05:01.76 Forearm.seek CC @04:05:03.63 CC.seek :closed @04:05:07.99 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.05 @04:05:08.01 Hand.open @04:05:08.87 Forearm.reconfigure LooseForearm @04:05:09.00 Forearm.seek :retract @04:05:10.07 SC.coast @04:05:10.15 collectSample 1500ml w/bubble point of 55psi @04:05:10.19 Priming 7ml @04:05:10.21 Intake.select :close @04:05:10.27 Exhaust.select :close @04:05:10.30 SS.hold @04:05:10.79 Calibrating pressure and temperature sensors @04:05:10.83 CTV.dial :air @04:05:10.85 SSV.dial :spit,avoiding: :exhaust @04:05:10.89 SS.reconfigure SSfast @04:05:10.99 SS.seek 5ml @04:05:13.73 SSV.dial CBV @04:05:13.79 CBV.dial SSV @04:05:15.30 delay 3.8 seconds @04:05:19.37 SSV.dial :spit @04:05:20.84 SS.seek 0ml @04:05:23.54 SSV.dial CBV @04:05:24.97 CTV.dial :intake @04:05:25.84 SSV.dial CBV @04:05:25.93 CBV.dial SSV @04:05:25.97 Allowing 40s for 7.0ml of remaining 7.0ml @04:05:25.99 Intake.select :open @04:05:28.25 Intake @31.1psia, vacuum from 20.0 to 24.0psi @04:05:28.30 SS.reconfigure SSinhale.with maxSpeed: 150 @04:05:28.43 SS.seek 7ml @04:05:32.75 Vacuum too high -- waiting up to 40s @04:05:37.76 Intake @30.9psia, vacuum from 20.0 to 24.0psi @04:05:37.84 SS.reconfigure SSinhale.with maxSpeed: 135 @04:05:37.94 SS.seek 7ml @04:05:40.66 Stroke completed -- waiting for pressure to equalize ... @04:05:43.92 Intake.select :close @04:05:44.16 SSV.dial :exhaust @04:05:45.04 Exhaust.select :open @04:05:45.25 SS.reconfigure SSexhale @04:05:45.35 SS.seek 0ml @04:05:49.08 Exhaust.select :close @04:05:49.24 Sampling 1500.0ml @04:05:49.27 Intake.select :close @04:05:49.29 Exhaust.select :close @04:05:49.32 SS.reconfigure SSconfig @04:05:49.40 SS.hold @04:05:49.86 CTV.dial :intake @04:05:49.90 SSV.dial CBV @04:05:49.97 CBV.dial SSV @04:05:50.83 Allowing 130s for 25.0ml of remaining 1500.0ml @04:05:50.86 Intake.select :open @04:05:53.14 Intake @31.4psia, vacuum from 20.0 to 24.0psi @04:05:53.18 SS.reconfigure SSinhale.with maxSpeed: 135 @04:05:53.27 SS.seek 25ml @04:05:57.56 Vacuum too high -- waiting up to 40s @04:06:00.35 Intake @30.7psia, vacuum from 20.0 to 24.0psi @04:06:00.42 SS.reconfigure SSinhale.with maxSpeed: 122 @04:06:00.54 SS.seek 25ml @04:06:04.18 Vacuum too high -- waiting up to 40s @04:06:07.25 Intake @30.8psia, vacuum from 20.0 to 24.0psi @04:06:07.27 SS.reconfigure SSinhale.with maxSpeed: 110 @04:06:07.32 SS.seek 25ml @04:06:10.77 Vacuum too high -- waiting up to 40s @04:06:13.59 Intake @30.8psia, vacuum from 20.0 to 24.0psi @04:06:13.61 SS.reconfigure SSinhale.with maxSpeed: 99 @04:06:13.66 SS.seek 25ml @04:06:16.89 Vacuum too high -- waiting up to 40s @04:06:19.62 Intake @30.8psia, vacuum from 20.0 to 24.0psi @04:06:19.64 SS.reconfigure SSinhale.with maxSpeed: 89 @04:06:19.69 SS.seek 25ml @04:06:22.93 Vacuum too high -- waiting up to 40s @04:06:25.76 Intake @30.9psia, vacuum from 20.0 to 24.0psi @04:06:25.78 SS.reconfigure SSinhale.with maxSpeed: 80 @04:06:25.83 SS.seek 25ml @04:06:28.83 Vacuum too high -- waiting up to 40s @04:06:31.46 SS.reconfigure SSinhale.with maxSpeed: 72 @04:06:31.51 SS.seek 25ml @04:06:34.71 Vacuum too high -- waiting up to 40s @04:06:37.54 SS.reconfigure SSinhale.with maxSpeed: 65 @04:06:37.59 SS.seek 25ml @04:06:38.76 Stroke completed -- waiting for pressure to equalize ... @04:06:43.20 Intake.select :close @04:06:43.36 SSV.dial :exhaust @04:06:44.78 Exhaust.select :open @04:06:44.90 SS.reconfigure SSexhale @04:06:44.95 SS.seek 0ml @04:06:53.94 Exhaust.select :close @04:06:54.06 SSV.dial CBV @04:06:54.08 CBV.dial SSV @04:06:54.92 Allowing 130s for 25.0ml of remaining 1475.0ml @04:06:54.93 Intake.select :open @04:06:57.11 Intake @31.5psia, vacuum from 20.0 to 24.0psi @04:06:57.14 SS.reconfigure SSinhale.with maxSpeed: 65 @04:06:57.19 SS.seek 25ml @04:07:02.30 Vacuum too high -- waiting up to 40s @04:07:04.39 Intake @30.9psia, vacuum from 20.0 to 24.0psi @04:07:04.41 SS.reconfigure SSinhale.with maxSpeed: 59 @04:07:04.46 SS.seek 25ml @04:07:08.27 Vacuum too high -- waiting up to 40s @04:07:10.84 Intake @30.9psia, vacuum from 20.0 to 24.0psi @04:07:10.86 SS.reconfigure SSinhale.with maxSpeed: 53 @04:07:10.91 SS.seek 25ml @04:07:16.21 Vacuum too high -- waiting up to 40s @04:07:19.03 Intake @31.0psia, vacuum from 20.0 to 24.0psi @04:07:19.05 SS.reconfigure SSinhale.with maxSpeed: 48 @04:07:19.10 SS.seek 25ml @04:07:25.04 Vacuum too high -- waiting up to 40s @04:07:27.91 SS.reconfigure SSinhale.with maxSpeed: 43 @04:07:28.00 SS.seek 25ml @04:07:35.45 Vacuum too high -- waiting up to 40s @04:07:38.17 Intake @30.9psia, vacuum from 20.0 to 24.0psi @04:07:38.19 SS.reconfigure SSinhale.with maxSpeed: 39 @04:07:38.24 SS.seek 25ml @04:07:46.93 Vacuum too high -- waiting up to 40s @04:07:49.75 Intake @31.0psia, vacuum from 20.0 to 24.0psi @04:07:49.77 SS.reconfigure SSinhale.with maxSpeed: 35 @04:07:49.82 SS.seek 25ml @04:07:55.02 Stroke completed -- waiting for pressure to equalize ... @04:08:01.96 Intake.select :close @04:08:02.12 SSV.dial :exhaust @04:08:02.94 Exhaust.select :open @04:08:03.06 SS.reconfigure SSexhale @04:08:03.10 SS.seek 0ml @04:08:12.20 Exhaust.select :close @04:08:12.32 SSV.dial CBV @04:08:12.34 CBV.dial SSV @04:08:13.17 Allowing 130s for 25.0ml of remaining 1450.0ml @04:08:13.18 Intake.select :open @04:08:15.37 Intake @31.4psia, vacuum from 20.0 to 24.0psi @04:08:15.39 SS.reconfigure SSinhale.with maxSpeed: 35 @04:08:15.44 SS.seek 25ml @04:08:29.49 Vacuum too high -- waiting up to 40s @04:08:31.81 Intake @30.9psia, vacuum from 20.0 to 24.0psi @04:08:31.83 SS.reconfigure SSinhale.with maxSpeed: 32 @04:08:31.88 SS.seek 25ml @04:08:49.01 Vacuum too high -- waiting up to 40s @04:08:51.97 Intake @31.0psia, vacuum from 20.0 to 24.0psi @04:08:51.99 SS.reconfigure SSinhale.with maxSpeed: 29 @04:08:52.03 SS.seek 25ml @04:09:18.04 Stroke completed -- waiting for pressure to equalize ... @04:09:27.52 Intake.select :close @04:09:27.68 SSV.dial :exhaust @04:09:28.50 Exhaust.select :open @04:09:28.62 SS.reconfigure SSexhale @04:09:28.66 SS.seek 0ml @04:09:37.89 Exhaust.select :close @04:09:38.01 SSV.dial CBV @04:09:38.03 CBV.dial SSV @04:09:38.86 Allowing 130s for 25.0ml of remaining 1425.0ml @04:09:38.87 Intake.select :open @04:09:41.04 Intake @31.4psia, vacuum from 20.0 to 24.0psi @04:09:41.06 SS.reconfigure SSinhale.with maxSpeed: 29 @04:09:41.11 SS.seek 25ml @04:10:08.87 Vacuum too high -- waiting up to 40s @04:10:11.19 Intake @30.9psia, vacuum from 20.0 to 24.0psi @04:10:11.21 SS.reconfigure SSinhale.with maxSpeed: 26 @04:10:11.26 SS.seek 25ml @04:10:48.10 Stroke completed -- waiting for pressure to equalize ... @04:10:57.57 Intake.select :close @04:10:57.73 SSV.dial :exhaust @04:10:58.55 Exhaust.select :open @04:10:58.67 SS.reconfigure SSexhale @04:10:58.72 SS.seek 0ml @04:11:07.70 Exhaust.select :close @04:11:07.82 SSV.dial CBV @04:11:07.86 CBV.dial SSV @04:11:08.64 Allowing 130s for 25.0ml of remaining 1400.0ml @04:11:08.65 Intake.select :open @04:11:10.84 Intake @31.4psia, vacuum from 20.0 to 24.0psi @04:11:10.86 SS.reconfigure SSinhale.with maxSpeed: 26 @04:11:10.90 SS.seek 25ml @04:11:57.70 Vacuum too high -- waiting up to 40s @04:12:00.29 Intake @31.0psia, vacuum from 20.0 to 24.0psi @04:12:00.31 SS.reconfigure SSinhale.with maxSpeed: 23 @04:12:00.36 SS.seek 25ml @04:12:24.14 Stroke completed -- waiting for pressure to equalize ... @04:12:32.93 Intake.select :close @04:12:33.09 SSV.dial :exhaust @04:12:33.89 Exhaust.select :open @04:12:34.01 SS.reconfigure SSexhale @04:12:34.05 SS.seek 0ml @04:12:43.77 Exhaust.select :close @04:12:43.89 SSV.dial CBV @04:12:43.91 CBV.dial SSV @04:12:44.75 Allowing 130s for 25.0ml of remaining 1375.0ml @04:12:44.76 Intake.select :open @04:12:46.93 Intake @31.3psia, vacuum from 20.0 to 24.0psi @04:12:46.95 SS.reconfigure SSinhale.with maxSpeed: 23 @04:12:47.00 SS.seek 25ml @04:14:03.11 Stroke completed -- waiting for pressure to equalize ... @04:14:13.15 Intake.select :close @04:14:13.31 SSV.dial :exhaust @04:14:14.14 Exhaust.select :open @04:14:14.26 SS.reconfigure SSexhale @04:14:14.31 SS.seek 0ml @04:14:23.46 Exhaust.select :close @04:14:23.58 SSV.dial CBV @04:14:23.60 CBV.dial SSV @04:14:24.44 Allowing 130s for 25.0ml of remaining 1350.0ml @04:14:24.45 Intake.select :open @04:14:26.64 Intake @31.4psia, vacuum from 20.0 to 24.0psi @04:14:26.66 SS.reconfigure SSinhale.with maxSpeed: 23 @04:14:26.71 SS.seek 25ml @04:15:43.07 Stroke completed -- waiting for pressure to equalize ... @04:15:55.02 Intake.select :close @04:15:55.18 SSV.dial :exhaust @04:15:56.00 Exhaust.select :open @04:15:56.12 SS.reconfigure SSexhale @04:15:56.17 SS.seek 0ml @04:16:05.39 Exhaust.select :close @04:16:05.51 SSV.dial CBV @04:16:05.53 CBV.dial SSV @04:16:06.38 Allowing 130s for 25.0ml of remaining 1325.0ml @04:16:06.39 Intake.select :open @04:16:08.56 Intake @31.4psia, vacuum from 20.0 to 24.0psi @04:16:08.58 SS.reconfigure SSinhale.with maxSpeed: 23 @04:16:08.63 SS.seek 25ml @04:16:54.20 Vacuum too high -- waiting up to 40s @04:16:57.27 Intake @30.9psia, vacuum from 20.0 to 24.0psi @04:16:57.29 SS.reconfigure SSinhale.with maxSpeed: 21 @04:16:57.34 SS.seek 25ml @04:17:31.09 Stroke completed -- waiting for pressure to equalize ... @04:17:42.48 Intake.select :close @04:17:42.64 SSV.dial :exhaust @04:17:43.46 Exhaust.select :open @04:17:43.58 SS.reconfigure SSexhale @04:17:43.63 SS.seek 0ml @04:17:52.91 Exhaust.select :close @04:17:53.03 SSV.dial CBV @04:17:53.05 CBV.dial SSV @04:17:53.89 Allowing 130s for 25.0ml of remaining 1300.0ml @04:17:53.90 Intake.select :open @04:17:56.07 Intake @31.3psia, vacuum from 20.0 to 24.0psi @04:17:56.09 SS.reconfigure SSinhale.with maxSpeed: 21 @04:17:56.14 SS.seek 25ml @04:18:55.59 Vacuum too high -- waiting up to 40s @04:18:59.16 Intake @31.1psia, vacuum from 20.0 to 24.0psi @04:18:59.18 SS.reconfigure SSinhale.with maxSpeed: 19 @04:18:59.23 SS.seek 25ml @04:19:26.10 Stroke completed -- waiting for pressure to equalize ... @04:19:38.63 Intake.select :close @04:19:38.79 SSV.dial :exhaust @04:19:39.61 Exhaust.select :open @04:19:39.73 SS.reconfigure SSexhale @04:19:39.78 SS.seek 0ml @04:19:49.21 Exhaust.select :close @04:19:49.33 SSV.dial CBV @04:19:49.35 CBV.dial SSV @04:19:50.19 Allowing 130s for 25.0ml of remaining 1275.0ml @04:19:50.20 Intake.select :open @04:19:52.39 Intake @31.3psia, vacuum from 20.0 to 24.0psi @04:19:52.41 SS.reconfigure SSinhale.with maxSpeed: 19 @04:19:52.45 SS.seek 25ml @04:21:05.21 Vacuum too high -- waiting up to 40s @04:21:09.04 Intake @31.2psia, vacuum from 20.0 to 24.0psi @04:21:09.06 SS.reconfigure SSinhale.with maxSpeed: 17 @04:21:09.11 SS.seek 25ml @04:21:31.26 Stroke completed -- waiting for pressure to equalize ... @04:21:44.42 Intake.select :close @04:21:44.58 SSV.dial :exhaust @04:21:45.39 Exhaust.select :open @04:21:45.51 SS.reconfigure SSexhale @04:21:45.55 SS.seek 0ml @04:21:54.57 Exhaust.select :close @04:21:54.69 SSV.dial CBV @04:21:54.71 CBV.dial SSV @04:21:55.54 Allowing 130s for 25.0ml of remaining 1250.0ml @04:21:55.55 Intake.select :open @04:21:57.72 Intake @31.4psia, vacuum from 20.0 to 24.0psi @04:21:57.74 SS.reconfigure SSinhale.with maxSpeed: 17 @04:21:58.41 SS.seek 25ml @04:23:41.88 Stroke completed -- waiting for pressure to equalize ... @04:23:58.93 Intake.select :close @04:23:59.09 SSV.dial :exhaust @04:23:59.91 Exhaust.select :open @04:24:00.03 SS.reconfigure SSexhale @04:24:00.07 SS.seek 0ml @04:24:09.18 Exhaust.select :close @04:24:09.30 SSV.dial CBV @04:24:09.32 CBV.dial SSV @04:24:10.16 Allowing 130s for 25.0ml of remaining 1225.0ml @04:24:10.17 Intake.select :open @04:24:12.35 Intake @31.4psia, vacuum from 20.0 to 24.0psi @04:24:12.37 SS.reconfigure SSinhale.with maxSpeed: 17 @04:24:12.42 SS.seek 25ml @04:25:10.28 Vacuum too high -- waiting up to 40s @04:25:14.36 Intake @31.2psia, vacuum from 20.0 to 24.0psi @04:25:14.38 SS.reconfigure SSinhale.with maxSpeed: 15 @04:25:14.43 SS.seek 25ml @04:26:06.54 Stroke completed -- waiting for pressure to equalize ... @04:26:20.18 SS.reconfigure SSconfig @04:26:20.23 SS.hold @04:26:20.36 Intake.select :close @04:26:20.52 ShallowSampler::Clogged in WCR -- progress too slow after 300.0ml @04:26:20.54 SSV.dial :exhaust @04:26:21.36 Exhaust.select :open @04:26:21.48 SS.reconfigure SSexhale @04:26:21.53 SS.seek 0ml @04:26:31.13 Exhaust.select :close @04:26:31.24 Sampled 300.0ml @04:26:31.26 Drying puck before fixing for 4:00 @04:26:31.27 CBV.dial :waste2b @04:26:32.07 delay 1:00 @04:27:32.10 CSV.dial :air @04:27:32.88 CS.seek 10ml @04:27:56.71 Pumping wcr puck from tube 3 up to 55psi... @04:27:56.72 CBV.dial SSV @04:27:58.09 CBV.dial between CSV and waste2b @04:27:59.11 CSV.dial between air and CTV @04:27:59.44 CS.seek 7.082ml @04:28:07.25 CTV.dial CSV,avoiding: :air @04:28:07.27 CSV.dial CTV @04:28:08.12 CS.reconfigure CSpressurize @04:28:08.17 CS.seek 0ml @04:28:26.93 CSV.dial :air @04:28:27.42 CS.reconfigure CSfast @04:28:27.47 CS.seek 10ml @04:28:51.18 CSV.dial between air and CTV @04:28:51.54 CS.seek 2.521ml @04:29:09.65 CTV.dial CSV,avoiding: :air @04:29:09.68 CSV.dial CTV @04:29:10.02 CS.reconfigure CSpressurize @04:29:10.07 CS.seek 0ml @04:29:14.52 CBV.dial :waste2b @04:29:14.88 CS.reconfigure CSconfig @04:29:14.93 CS.seek :empty @04:29:18.69 delay 1:00 @04:30:18.72 CTV.dial :air,avoiding: :intake @04:30:20.12 Fix #1 of 2 (with rnal) for 2:00 @04:30:20.14 CSV.dial CSR @04:30:21.26 Pulling 2ml of CSR.rnal @04:30:21.28 CSR.select :rnal @04:30:21.40 CS.seek 2ml @04:30:27.56 delay 5 seconds @04:30:32.59 CSR.select :air @04:30:32.72 CS.reconfigure CSfast @04:30:32.77 CS.seek 10ml @04:30:51.90 Pumping wcr puck from tube 3 up to 55psi... @04:30:51.92 CBV.dial SSV @04:30:53.29 CBV.dial between CSV and waste2b @04:30:54.33 CSV.dial between air and CTV @04:30:54.98 CS.seek 7.237ml @04:31:02.38 CTV.dial CSV,avoiding: :air @04:31:02.40 CSV.dial CTV @04:31:03.88 CS.reconfigure CSpressurize @04:31:03.93 CS.seek 0ml @04:31:23.09 CSV.dial :air @04:31:23.59 CS.reconfigure CSfast @04:31:23.64 CS.seek 10ml @04:31:47.43 CSV.dial between air and CTV @04:31:47.79 CS.seek 2.175ml @04:32:06.66 CTV.dial CSV,avoiding: :air @04:32:06.68 CSV.dial CTV @04:32:07.02 CS.reconfigure CSpressurize @04:32:07.07 CS.seek 0ml @04:32:09.11 delay 2:00 @04:34:09.14 CBV.dial :waste2b @04:34:09.49 CS.reconfigure CSconfig @04:34:09.54 CS.seek :empty @04:34:14.50 delay 1:00 @04:35:14.53 CTV.dial :air,avoiding: :intake @04:35:15.94 Fix #2 of 2 (with rnal) for 2:00 @04:35:15.96 CSV.dial CSR @04:35:16.48 Pulling 2ml of CSR.rnal @04:35:16.50 CSR.select :rnal @04:35:16.62 CS.seek 2ml @04:35:22.72 delay 5 seconds @04:35:27.75 CSR.select :air @04:35:27.88 CS.reconfigure CSfast @04:35:27.93 CS.seek 10ml @04:35:47.00 Pumping wcr puck from tube 3 up to 55psi... @04:35:47.01 CBV.dial SSV @04:35:48.38 CBV.dial between CSV and waste2b @04:35:49.42 CSV.dial between air and CTV @04:35:50.18 CS.seek 7.258ml @04:35:57.51 CTV.dial CSV,avoiding: :air @04:35:57.53 CSV.dial CTV @04:35:59.00 CS.reconfigure CSpressurize @04:35:59.05 CS.seek 0ml @04:36:18.25 CSV.dial :air @04:36:18.72 CS.reconfigure CSfast @04:36:18.78 CS.seek 10ml @04:36:42.31 CSV.dial between air and CTV @04:36:43.26 CS.seek 2.213ml @04:37:01.98 CTV.dial CSV,avoiding: :air @04:37:02.00 CSV.dial CTV @04:37:02.36 CS.reconfigure CSpressurize @04:37:02.41 CS.seek 0ml @04:37:04.67 delay 2:00 @04:39:04.71 CBV.dial :waste2b @04:39:05.05 CS.reconfigure CSconfig @04:39:05.10 CS.seek :empty @04:39:10.05 delay 1:00 @04:40:10.08 CTV.dial :air,avoiding: :intake @04:40:11.55 Storing wcr puck in Collection Clamp to tube 2 @04:40:11.74 SC.reconfigure SCtight @04:40:11.79 SC.seek 2 @04:40:13.96 SC.reconfigure SCloose @04:40:14.19 Elbow.reconfigure TightElbow @04:40:14.24 Elbow.seek 2 @04:40:16.19 Forearm.reconfigure TightForearm @04:40:16.25 Forearm.seek 2 + 700 counts @04:40:17.35 SE.reconfigure SEpick @04:40:17.43 SE.seek beamBreak @04:40:20.40 SE.configure SEconfig @04:40:20.57 Elbow.reconfigure LimpElbow @04:40:20.63 Forearm.reconfigure LooseForearm @04:40:20.67 Forearm.seek 2 @04:40:20.97 Elbow.hold @04:40:21.05 Elbow.reconfigure TightElbow @04:40:21.17 SE.seek 1.07 Pucks below Top Plate @04:40:21.61 Forearm.force -20 @04:40:21.66 Forearm.seek 2 + 335 counts @04:40:22.00 Forearm.reconfigure TightForearm @04:40:22.05 Forearm.seek 2 + 700 counts @04:40:22.32 SE.seek 2.07 Pucks below Top Plate @04:40:22.90 Forearm.reconfigure LooseForearm @04:40:22.95 Forearm.seek :retract @04:40:23.89 Elbow.seek CC @04:40:25.61 Elbow.reconfigure LooseElbow @04:40:25.72 Forearm.seek CC @04:40:26.77 Hand.close @04:40:27.39 CC.seek :open @04:40:31.88 Forearm.seek :retract @04:40:32.92 Elbow.reconfigure TightElbow @04:40:32.97 Elbow.seek 2 @04:40:34.93 Elbow.reconfigure LimpElbow @04:40:35.59 Forearm.seek 2 @04:40:36.64 Elbow.hold @04:40:36.72 Elbow.reconfigure TightElbow @04:40:36.77 Hand.open @04:40:37.52 Forearm.force -20 @04:40:37.57 Forearm.seek 2 + 346 counts @04:40:37.89 Forearm.reconfigure TightForearm @04:40:37.94 Forearm.seek 2 + 700 counts @04:40:38.34 SE.seek :down @04:40:39.49 Forearm.reconfigure LooseForearm @04:40:39.54 Forearm.seek :retract @04:40:40.97 SE.coast @04:40:41.01 SC.coast @04:40:41.05 Starting finalPurge @04:40:41.07 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @04:40:41.15 Hand.open @04:40:41.52 Elbow.seek FlushPuck::Garage @04:40:43.56 Elbow.reconfigure LooseElbow @04:40:43.66 Forearm.seek :garage @04:40:44.66 Hand.close @04:40:45.40 Forearm.seek :retract @04:40:46.42 Elbow.reconfigure TightElbow @04:40:46.47 Elbow.seek CC @04:40:49.42 Elbow.reconfigure LooseElbow @04:40:49.53 Forearm.reconfigure TightForearm @04:40:49.58 Forearm.seek CC @04:40:50.91 CC.seek :closed @04:40:55.16 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.96 @04:40:55.24 Hand.open @04:40:55.96 Forearm.reconfigure LooseForearm @04:40:56.01 Forearm.seek :retract @04:40:57.02 Flushing the Collection Valve::Manifold @04:40:57.16 CSV.dial CSR @04:40:57.73 Pulling 5ml of CSR.flush @04:40:57.74 CSR.select :flush @04:40:57.86 CS.seek 5ml @04:41:11.73 delay 5 seconds @04:41:16.76 CSR.select :air @04:41:16.92 CS.reconfigure CSfast @04:41:16.97 CS.seek 7.501ml @04:41:23.73 CSV.dial :waste @04:41:25.21 CS.reconfigure CSconfig @04:41:25.26 CS.seek 0ml @04:41:45.60 Flushing lower manifold's waste1 port @04:41:45.73 CSV.dial :flush @04:41:46.21 CS.reconfigure CSfast @04:41:46.88 CS.seek 5ml @04:41:59.26 delay 5 seconds @04:42:04.29 CSV.dial :air @04:42:04.83 CS.seek 7ml @04:42:10.39 CSV.dial CTV @04:42:10.42
CTV.dial CSV @04:42:11.90 CBV.dial :waste1 @04:42:12.71 CS.seek 0ml @04:42:29.51 Flushing lower manifold's waste2b port @04:42:29.65 CSV.dial :flush @04:42:30.44 CS.seek 5ml @04:42:42.67 delay 5 seconds @04:42:47.70 CSV.dial :air @04:42:48.25 CS.seek 7ml @04:42:53.79 CSV.dial CTV @04:42:53.83
CTV.dial CSV @04:42:54.29 CBV.dial :waste2b @04:42:55.10 CS.seek 0ml @04:43:11.86 Flushing upper manifold @04:43:11.99 CSV.dial :flush @04:43:12.79 CS.seek 5ml @04:43:25.14 delay 5 seconds @04:43:30.17 CSV.dial :air @04:43:30.72 CS.seek 7ml @04:43:36.29 CSV.dial CBV @04:43:36.33
CBV.dial CSV @04:43:37.45 CTV.dial :air @04:43:38.85 CS.seek 0ml @04:43:55.54 delay 7 seconds @04:44:02.58 CS.seek 3ml @04:44:10.39 CSV.dial :waste @04:44:10.88 CS.seek 0ml @04:44:18.76 CSV.dial :flush @04:44:19.23 CS.seek 3ml @04:44:27.04 delay 5 seconds @04:44:32.07 CSV.dial :air @04:44:32.61 CS.seek 5ml @04:44:38.17 CSV.dial :air @04:44:38.19 CS.seek 0ml @04:44:50.55 delay 7 seconds @04:44:57.59 CS.seek 3ml @04:45:05.51 CSV.dial :waste @04:45:06.29 CS.seek 0ml @04:45:14.14 Disinfecting external Intake with mfbkill @04:45:14.27 CSV.dial CSR @04:45:15.68 Pulling 8ml of CSR.mfbkill @04:45:15.69 CSR.select :mfbkill @04:45:15.81 CS.seek 8ml @04:45:34.86 delay 5 seconds @04:45:39.89 CSR.select :air @04:45:40.02 CS.seek 10ml @04:45:45.67 CSV.dial CBV @04:45:45.71
CBV.dial CSV @04:45:46.78 CTV.dial :intake @04:45:47.58 Intake.select :open @04:45:47.70 CS.seek 2ml @04:46:07.92 Intake.select :close @04:46:08.03 CTV.dial :air @04:46:08.83 CS.reconfigure CSconfig @04:46:08.88 CS.seek 0ml @04:46:14.98 Flushing lower manifold's waste1 port @04:46:14.99 CSV.dial CSR @04:46:16.25 Pulling 4ml of CSR.mfbkill @04:46:16.26 CSR.select :mfbkill @04:46:16.38 CS.seek 4ml @04:46:27.64 delay 5 seconds @04:46:32.67 CSR.select :air @04:46:32.79 CS.reconfigure CSfast @04:46:32.85 CS.seek 10ml @04:46:47.36 CSV.dial CTV @04:46:47.40
CTV.dial CSV @04:46:48.86 CBV.dial :waste1 @04:46:49.96 CS.seek 0ml @04:47:13.52 Flushing lower manifold's waste2b port @04:47:13.53 CSV.dial CSR @04:47:14.08 Pulling 4ml of CSR.mfbkill @04:47:14.09 CSR.select :mfbkill @04:47:14.22 CS.reconfigure CSconfig @04:47:14.27 CS.seek 4ml @04:47:25.58 delay 5 seconds @04:47:30.61 CSR.select :air @04:47:30.74 CS.reconfigure CSfast @04:47:30.78 CS.seek 10ml @04:47:45.41 CSV.dial CTV @04:47:45.44
CTV.dial CSV @04:47:45.94 CBV.dial :waste2b @04:47:46.76 CS.seek 0ml @04:48:10.49 Killing upper manifold @04:48:10.50 CSV.dial CSR @04:48:11.05 Pulling 4ml of CSR.mfbkill @04:48:11.06 CSR.select :mfbkill @04:48:11.18 CS.reconfigure CSconfig @04:48:11.23 CS.seek 4ml @04:48:22.36 delay 5 seconds @04:48:27.39 CSR.select :air @04:48:27.51 CS.reconfigure CSfast @04:48:27.55 CS.seek 10ml @04:48:42.30 CSV.dial CBV @04:48:42.33
CBV.dial CSV @04:48:43.46 CTV.dial :air @04:48:44.86 CS.seek 0ml @04:49:08.30 delay 7 seconds @04:49:15.34 CS.seek 3ml @04:49:23.14 CSV.dial :waste @04:49:23.61 CS.seek 0ml @04:49:31.64 Flushing upper manifold @04:49:31.77 CSV.dial :flush @04:49:32.26 CS.seek 5ml @04:49:44.64 delay 5 seconds @04:49:49.67 CSV.dial :air @04:49:50.21 CS.seek 7ml @04:49:55.93 CSV.dial CBV @04:49:55.96
CBV.dial CSV @04:49:57.03 CTV.dial :air @04:49:57.05 CS.seek 0ml @04:50:13.80 delay 7 seconds @04:50:20.84 CS.seek 3ml @04:50:28.65 CSV.dial :waste @04:50:29.26 CS.seek 0ml @04:50:37.39 CSV.dial :flush @04:50:37.87 CS.seek 3ml @04:50:45.65 delay 5 seconds @04:50:50.68 CSV.dial :air @04:50:51.34 CS.seek 5ml @04:50:56.93 CSV.dial :air @04:50:56.95 CS.seek 0ml @04:51:09.26 delay 7 seconds @04:51:16.31 CS.seek 3ml @04:51:24.21 CSV.dial :waste @04:51:24.97 CS.seek 0ml @04:51:32.83 Flushing lower manifold's waste1 port @04:51:32.96 CSV.dial :flush @04:51:33.45 CS.seek 5ml @04:51:45.74 delay 5 seconds @04:51:50.77 CSV.dial :air @04:51:51.31 CS.seek 7ml @04:51:56.99 CSV.dial CTV @04:51:57.03
CTV.dial CSV @04:51:58.49 CBV.dial :waste1 @04:51:58.98 CS.seek 0ml @04:52:15.74 Flushing lower manifold's waste2b port @04:52:15.87 CSV.dial :flush @04:52:16.66 CS.seek 5ml @04:52:29.59 delay 5 seconds @04:52:34.62 CSV.dial :air @04:52:35.17 CS.seek 7ml @04:52:40.74 CSV.dial CTV @04:52:40.77
CTV.dial CSV @04:52:41.24 CBV.dial :waste2b @04:52:42.06 CS.seek 0ml @04:52:58.87 Disinfecting external Intake with kill @04:52:59.01 CSV.dial CSR @04:52:59.54 Pulling 8ml of CSR.kill @04:52:59.55 CSR.select :kill @04:52:59.68 CS.seek 8ml @04:53:18.71 delay 5 seconds @04:53:23.74 CSR.select :air @04:53:23.87 CS.seek 10ml @04:53:29.41 CSV.dial CBV @04:53:29.45
CBV.dial CSV @04:53:30.57 CTV.dial :intake @04:53:31.34 Intake.select :open @04:53:31.46 CS.seek 2ml @04:53:52.21 Intake.select :close @04:53:52.32 CTV.dial :air @04:53:53.10 CS.reconfigure CSconfig @04:53:53.15 CS.seek 0ml @04:53:59.31 CSV.dial CSR @04:54:00.44 Pulling 5ml of CSR.flush @04:54:00.45 CSR.select :flush @04:54:00.57 CS.seek 5ml @04:54:14.34 delay 5 seconds @04:54:19.37 CSR.select :air @04:54:19.50 CS.seek 10ml @04:54:33.46 CSV.dial CBV @04:54:34.54 CS.seek 0ml @04:55:00.99 Garaging FlushPuck in Collection Clamp @04:55:01.07 Elbow.reconfigure TightElbow @04:55:01.12 Elbow.seek CC @04:55:01.38 Elbow.reconfigure LooseElbow @04:55:01.49 Forearm.seek CC @04:55:02.48 Hand.close @04:55:03.04 CC.seek :open @04:55:07.44 Forearm.seek :retract @04:55:08.43 Elbow.reconfigure TightElbow @04:55:08.48 Elbow.seek :garage @04:55:11.68 Elbow.reconfigure LooseElbow @04:55:11.78 Forearm.reconfigure TightForearm @04:55:11.83 Forearm.seek :garage @04:55:12.99 Hand.open @04:55:13.74 Forearm.reconfigure LooseForearm @04:55:13.79 Forearm.seek :retract @04:55:14.81 Starting initialPurge @04:55:14.82 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @04:55:14.90 Hand.open @04:55:15.24 Elbow.reconfigure TightElbow @04:55:15.28 Elbow.seek FlushPuck::Garage @04:55:15.48 Elbow.reconfigure LooseElbow @04:55:15.59 Forearm.seek :garage @04:55:16.59 Hand.close @04:55:17.30 Forearm.seek :retract @04:55:18.35 Elbow.reconfigure TightElbow @04:55:18.40 Elbow.seek CC @04:55:21.35 Elbow.reconfigure LooseElbow @04:55:21.45 Forearm.reconfigure TightForearm @04:55:21.50 Forearm.seek CC @04:55:23.44 CC.seek :closed @04:55:27.82 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.03 @04:55:27.91 Hand.open @04:55:28.62 Forearm.reconfigure LooseForearm @04:55:28.67 Forearm.seek :retract @04:55:29.67 Flushing the Collection Valve::Manifold @04:55:29.81 CSV.dial CSR @04:55:30.95 Pulling 5ml of CSR.flush @04:55:30.96 CSR.select :flush @04:55:31.09 CS.seek 5ml @04:55:44.90 delay 5 seconds @04:55:49.93 CSR.select :air @04:55:50.09 CS.reconfigure CSfast @04:55:50.14 CS.seek 7.501ml @04:55:56.87 CSV.dial :waste @04:55:58.21 CS.reconfigure CSconfig @04:55:58.26 CS.seek 0ml @04:56:18.40 Purging external Intake @04:56:18.53 CSV.dial :flush @04:56:19.01 CS.reconfigure CSfast @04:56:19.06 CS.seek 10ml @04:56:42.62 delay 5 seconds @04:56:47.65 CSV.dial CBV @04:56:47.68
CBV.dial CSV @04:56:48.47 CTV.dial :intake @04:56:49.28 Intake.select :open @04:56:49.40 CS.seek 0ml @04:57:14.28 Intake.select :close @04:57:14.39 CTV.dial :air @04:57:15.20 Flushing upper manifold @04:57:15.33 CSV.dial :flush @04:57:16.12 CS.seek 5ml @04:57:28.43 delay 5 seconds @04:57:33.46 CSV.dial :air @04:57:34.13 CS.seek 7ml @04:57:40.00 CSV.dial CBV @04:57:40.02
CBV.dial CSV @04:57:41.09 CTV.dial :air @04:57:41.11 CS.seek 0ml @04:57:57.87 delay 7 seconds @04:58:04.91 CS.seek 3ml @04:58:12.81 CSV.dial :waste @04:58:13.29 CS.seek 0ml @04:58:21.21 CSV.dial :flush @04:58:21.68 CS.seek 3ml @04:58:29.47 delay 5 seconds @04:58:34.49 CSV.dial :air @04:58:35.02 CS.seek 5ml @04:58:40.56 CSV.dial :air @04:58:40.58 CS.seek 0ml @04:58:52.84 delay 7 seconds @04:58:59.88 CS.seek 3ml @04:59:07.78 CSV.dial :waste @04:59:08.56 CS.seek 0ml @04:59:16.31 Garaging FlushPuck in Collection Clamp @04:59:16.39 Elbow.reconfigure TightElbow @04:59:16.44 Elbow.seek CC @04:59:16.66 Elbow.reconfigure LooseElbow @04:59:16.77 Forearm.seek CC @04:59:17.77 Hand.close @04:59:18.42 CC.seek :open @04:59:22.79 Forearm.seek :retract @04:59:23.78 Elbow.reconfigure TightElbow @04:59:23.83 Elbow.seek :garage @04:59:26.99 Elbow.reconfigure LooseElbow @04:59:27.09 Forearm.reconfigure TightForearm @04:59:27.14 Forearm.seek :garage @04:59:28.29 Hand.open @04:59:29.61 Forearm.reconfigure LooseForearm @04:59:29.66 Forearm.seek :retract @04:59:30.66 Completed [wcrphase] begun 04:00:25 @04:59:30.68 Preparing to sleep until 10:00 21-may-20 @04:59:30.75 SS.coast @04:59:30.77
Asleep til 10:00 21-may-20 @04:59:30.85 Continuing at 10:00 @04:59:30.86 CC.coast @04:59:30.98 GoodNews.email "Then, in tube 3: coreNEG\n/home/esp/esp2/mission/20invasivedeployfinal.rb:145\n{1 => 22, 2 => 3, 3 => 19}\nCan@04:59:30, 9.5C, 45% humidity, 14.4psia, 13.549V, 0.487A, 0.481A avg, 6.60W",Subject: "Asleep until 10:00" @04:59:31.01 CS.coast @04:59:31.11 PC.coast @04:59:31.15 Forearm.coast @04:59:31.19 Elbow.coast @04:59:31.58 Gate.power :core,:OFF @04:59:31.60 Gate.power :raw,:OFF @04:59:31.61 Shutdown @10:00:00.06
Powering core bus @10:00:04.87 Gate.power :raw,:ON @10:00:05.52 SPE.coast @10:00:05.59 SPE.configure SPEconfig @10:00:05.69 AS.configure ASconfig @10:00:05.79 SS.configure SSconfig @10:00:05.89 CC.configure CCconfig @10:00:05.99 CS.configure CSconfig @10:00:06.09 PC.configure PCconfig @10:00:06.19 PS.configure PSconfig @10:00:06.31 Forearm.configure LooseForearm @10:00:06.41 Elbow.configure LooseElbow @10:00:06.53 SC.configure SCloose @10:00:06.62 SE.configure SEconfig @10:00:06.67 CH.coast @10:00:06.73 CH.configure CHconfig @10:00:06.76 PH.coast @10:00:06.82 PH.configure PHconfig @10:00:06.86 PCR.off @10:00:06.89 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:06.93 PCR.caltemp= 2.8814,0.0372,0 @10:00:06.96 All dwarf controllers running firmware version 3.75 @10:00:07.07 Valve.reset @10:00:07.24 PCR.off @10:00:07.27 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:07.30 PCR.caltemp= 2.8814,0.0372,0 @10:00:07.54 Intake.select :close @10:00:07.55 Exhaust.select :close @10:00:07.56 CTV.dial :air @10:00:07.64 PSV.dial :air @10:00:07.80 CSV.dial :waste1 @10:00:07.84 PS.reconfigure PSconfig @10:00:07.86
Forearm.reconfigure LooseForearm @10:00:07.99 PS.home.seek 0ml @10:00:08.06
Forearm.home.seek :retract @10:00:08.12 SSV.dial :spit,avoiding: :exhaust @10:00:08.83 Hand.close @10:00:08.86
Elbow.jog 1500 @10:00:08.99 CS.reconfigure CSfast @10:00:09.02 SS.reconfigure SSconfig @10:00:09.12 CS.home.seek 0ml @10:00:09.15 SS.home.seek 0ml @10:00:09.58
Elbow.reconfigure LooseElbow @10:00:09.62 Elbow.home.seek :clear @10:00:13.34 SE.reconfigure SEconfig @10:00:13.40 SE.home.seek :down @10:00:14.13 SC.reconfigure SCsloppy @10:00:14.18 SC.home.seek :initial @10:00:17.03 PS.coast @10:00:18.37
Forearm.seek :retract @10:00:18.45 Elbow.seek :hammer @10:00:19.74 Forearm.reconfigure TightForearm @10:00:19.79 Forearm.seek 5 + 350 counts @10:00:20.81 Forearm.seek :retract @10:00:22.03 PC.reconfigure PCconfig @10:00:22.05
CC.reconfigure CCconfig @10:00:22.14 PC.home.seek :open @10:00:22.17
CC.home.seek :open @10:00:25.67 Phase [coreNEG] @10:00:25.68 GoodNews.email "At 10:00:25 began in tube 3: coreNEG\n/home/esp/esp2/mission/20invasivedeployfinal.rb:145\n{1 => 22, 2 => 3, 3 => 19}\nCan@10:00:25, 9.3C, 45% humidity, 14.4psia, 13.589V, 0.544A, 0.537A avg, 7.39W",Subject: "Started 20invasivedeployfinal phase coreNEG" @10:00:25.70CEST21-May-20
===> STARTED coreNEG (phase #11) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @10:00:25.72 Starting initialPurge @10:00:25.76 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:00:25.84 Hand.open @10:00:26.61 Elbow.reconfigure TightElbow @10:00:26.66 Elbow.seek FlushPuck::Garage @10:00:28.66 Elbow.reconfigure LooseElbow @10:00:28.77 Forearm.reconfigure LooseForearm @10:00:28.82 Forearm.seek :garage @10:00:29.83 Hand.close @10:00:30.73 Forearm.seek :retract @10:00:31.78 Elbow.reconfigure TightElbow @10:00:31.89 Elbow.seek CC @10:00:35.05 Elbow.reconfigure LooseElbow @10:00:35.28 Forearm.reconfigure TightForearm @10:00:35.41 Forearm.seek CC @10:00:37.24 CC.seek :closed @10:00:41.70 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.10 @10:00:41.86 Hand.open @10:00:42.64 Forearm.reconfigure LooseForearm @10:00:42.72 Forearm.seek :retract @10:00:43.78 Flushing the Collection Valve::Manifold @10:00:44.08 CSV.dial CSR @10:00:45.64 Pulling 5ml of CSR.flush @10:00:45.66 CSR.select :flush @10:00:45.87 CS.reconfigure CSconfig @10:00:45.97 CS.seek 5ml @10:00:59.78 delay 5 seconds @10:01:04.81 CSR.select :air @10:01:05.08 CS.reconfigure CSfast @10:01:05.20 CS.seek 7.5ml @10:01:11.97 CSV.dial :waste @10:01:13.42 CS.reconfigure CSconfig @10:01:13.52 CS.seek 0ml @10:01:33.72 Purging external Intake @10:01:33.86 CSV.dial :flush @10:01:34.35 CS.reconfigure CSfast @10:01:34.40 CS.seek 10ml @10:01:57.94 delay 5 seconds @10:02:02.97 CSV.dial CBV @10:02:03.01
CBV.dial CSV @10:02:03.80 CTV.dial :intake @10:02:04.58 Intake.select :open @10:02:04.70 CS.seek 0ml @10:02:29.85 Intake.select :close @10:02:29.96 CTV.dial :air @10:02:30.76 Flushing upper manifold @10:02:30.89 CSV.dial :flush @10:02:31.67 CS.seek 5ml @10:02:43.97 delay 5 seconds @10:02:49.00 CSV.dial :air @10:02:50.12 CS.seek 7ml @10:02:55.63 CSV.dial CBV @10:02:55.65
CBV.dial CSV @10:02:56.74 CTV.dial :air @10:02:56.76 CS.seek 0ml @10:03:13.57 delay 7 seconds @10:03:20.61 CS.seek 3ml @10:03:28.51 CSV.dial :waste @10:03:29.13 CS.seek 0ml @10:03:37.03 CSV.dial :flush @10:03:37.52 CS.seek 3ml @10:03:45.32 delay 5 seconds @10:03:50.35 CSV.dial :air @10:03:50.89 CS.seek 5ml @10:03:56.44 CSV.dial :air @10:03:56.46 CS.seek 0ml @10:04:08.94 delay 7 seconds @10:04:15.98 CS.seek 3ml @10:04:23.88 CSV.dial :waste @10:04:24.66 CS.seek 0ml @10:04:32.51 Garaging FlushPuck in Collection Clamp @10:04:32.59 Elbow.reconfigure TightElbow @10:04:32.64 Elbow.seek CC @10:04:32.92 Elbow.reconfigure LooseElbow @10:04:33.03 Forearm.seek CC @10:04:34.01 Hand.close @10:04:34.59 CC.seek :open @10:04:38.95 Forearm.seek :retract @10:04:39.95 Elbow.reconfigure TightElbow @10:04:40.00 Elbow.seek :garage @10:04:43.17 Elbow.reconfigure LooseElbow @10:04:43.32 Forearm.reconfigure TightForearm @10:04:43.36 Forearm.seek :garage @10:04:44.48 Hand.open @10:04:45.16 Forearm.reconfigure LooseForearm @10:04:45.20 Forearm.seek :retract @10:04:46.33 Starting mfb @10:04:46.34 pcrBAC wcr sampling at most 1500ml @10:04:46.38 BACfans.email "Started mfb in tube 3 at 10:04:46CEST21-May-20\n{1 => 22, 2 => 3, 3 => 19}\nCan@10:00:25, 9.3C, 45% humidity, 14.4psia, 13.589V, 0.544A, 0.537A avg, 7.39W",Subject: "pcrBAC wcr sampling at most 1500ml" @10:04:46.48
Loading sh1 puck from tube 3 into Collection Clamp @10:04:46.59 Readying the MFB @10:04:46.62 Homing MFB Syringe @10:04:46.64 AFV.select :holdingCoil @10:04:46.92 ATV.dial :air2 @10:04:46.95
SC.reconfigure SCtight @10:04:47.06 SC.seek 3 @10:04:47.53 AS.reconfigure ASconfig @10:04:47.58 AS.home.seek :home @10:04:49.26
SC.reconfigure SCloose @10:04:49.53 Elbow.reconfigure TightElbow @10:04:49.58 Elbow.seek 3 @10:04:51.82 Forearm.reconfigure TightForearm @10:04:51.94 Forearm.seek 3 + 700 counts @10:04:53.05 SE.reconfigure SEpick @10:04:53.12 AFV.select :carrierSolution @10:04:53.17
SE.seek beamBreak @10:04:53.23 delay 2 seconds @10:04:54.61
SE.configure SEconfig @10:04:54.99 Elbow.seek 3 @10:04:55.40 AS.seek 1ml @10:04:55.59
Forearm.seek 3 + 300 counts @10:04:55.94 Forearm.reconfigure LooseForearm @10:04:56.03 Hand.close @10:04:56.82 SE.seek :down @10:04:57.37
Forearm.seek :retract @10:04:58.23 SE.coast @10:04:58.40
Elbow.seek CC @10:05:00.27 Elbow.reconfigure LooseElbow @10:05:00.57 Forearm.reconfigure TightForearm @10:05:00.70 Forearm.seek CC @10:05:02.49 CC.seek :closed @10:05:06.90 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.10 @10:05:06.92 Hand.open @10:05:07.75 Forearm.reconfigure LooseForearm @10:05:07.88 Forearm.seek :retract @10:05:08.97 SC.coast @10:05:09.05 skipping prime and sample collection @10:05:09.08 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:05:09.13 Intake.select :close @10:05:09.30 CSV.dial :waste @10:05:09.46 CS.seek 0ml @10:05:09.68 CSV.dial :air @10:05:09.83
Calibrating pressure and temperature sensors @10:05:09.92 CTV.dial :air @10:05:09.95 SSV.dial :spit,avoiding: :exhaust @10:05:10.03 SS.reconfigure SSfast @10:05:10.15 SS.seek 5ml @10:05:10.69 CS.seek 10ml @10:05:12.87 SSV.dial CBV @10:05:12.95
CBV.dial SSV @10:05:14.45 delay 3.8 seconds @10:05:18.52 SSV.dial :spit @10:05:19.99 SS.seek 0ml @10:05:22.67 SSV.dial CBV @10:05:24.13 SSV.dial CBV,avoiding: :exhaust @10:05:24.18
CBV.dial SSV @10:05:24.22 CTV.dial :air @10:05:24.25 delay 15 seconds @10:05:39.32 SSV.dial :spit,avoiding: :exhaust @10:05:40.78 CTV.dial CSV,avoiding: :intake @10:05:40.85
SS.reconfigure SSexhale @10:05:41.07 SS.seek 0ml @10:05:41.21 SSV.dial CBV,avoiding: :exhaust @10:05:42.83 CBV.dial between SSV and waste2 @10:05:44.69 CTV.dial CSV,avoiding: :air @10:05:44.75
CSV.dial CTV @10:05:45.32 CS.reconfigure CSpressurize @10:05:45.42 CS.seek 0ml @10:05:48.39 delay 1 second @10:05:49.42 AFV.select :holdingCoil @10:05:49.55 delay 1 second @10:05:50.59 ATV.dial :waste @10:05:51.68 AS.reconfigure ASnorm @10:05:51.78 AS.home.seek :empty @10:06:11.64
CSV.dial :air @10:06:12.15 CS.reconfigure CSfast @10:06:12.19 CS.seek 10ml @10:06:35.79 CSV.dial between air and CTV @10:06:36.15 CS.seek 3.343ml @10:06:44.34 ATV.dial :waste @10:06:44.35 ARV.dial between carrier and air1 @10:06:44.45 ASV.dial :waste1 @10:06:52.20 CTV.dial CSV,avoiding: :air @10:06:52.23
CSV.dial CTV @10:06:52.57 CS.reconfigure CSpressurize @10:06:52.61 CS.seek 0ml @10:07:01.85 CSV.dial :air @10:07:02.32 CS.reconfigure CSfast @10:07:02.37 CS.seek 10ml @10:07:25.88 CSV.dial between air and CTV @10:07:26.24 CS.seek 2.067ml @10:07:45.37 CTV.dial CSV,avoiding: :air @10:07:45.40
CSV.dial CTV @10:07:45.72 CS.reconfigure CSpressurize @10:07:45.76 CS.seek 0ml @10:07:46.91 BubblePoint: Intake @69.6psia, Outlet @14.4psia @10:07:46.92 CBV.dial SSV @10:07:47.26 SS.reconfigure SSconfig @10:07:47.31 SS.seek 10ml @10:07:53.03 delay 15 seconds @10:08:08.16 Evacuated: Intake @38.5psia, Outlet @22.8psia @10:08:08.17 SSV.dial :spit,avoiding: :exhaust @10:08:09.57 SS.reconfigure SSexhale @10:08:09.62 SS.seek 0ml @10:08:14.38 SS.reconfigure SSfast @10:08:14.43 SS.seek 5ml @10:08:17.04 SSV.dial CBV,avoiding: :exhaust @10:08:17.07
CBV.dial SSV @10:08:18.48 CTV.dial :air,avoiding: :intake @10:08:19.89 CSV.dial CTV @10:08:19.94
SS.seek 10ml @10:08:20.94 CSV.dial :air @10:08:21.43 CS.reconfigure CSfast @10:08:21.48 CS.seek 0ml @10:08:22.61
SSV.dial :spit,avoiding: :exhaust @10:08:24.01 SS.reconfigure SSexhale @10:08:24.06 SS.seek 0ml @10:08:26.85 CS.coast @10:08:29.05
SS.reconfigure SSfast @10:08:29.10 SS.seek 5ml @10:08:31.78 SSV.dial CBV,avoiding: :exhaust @10:08:31.81
CBV.dial SSV @10:08:34.24 SSV.dial :spit,avoiding: :exhaust @10:08:35.64 SS.reconfigure SSconfig @10:08:35.69 SS.seek 0ml @10:08:38.81 SS.coast @10:08:38.85 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:08:38.86 Intake.select :close @10:08:39.53 CSV.dial :waste @10:08:39.67 SSV.dial CBV,avoiding: :exhaust @10:08:39.69
CBV.dial SSV @10:08:40.38 CS.seek 0ml @10:08:40.45 CSV.dial :air @10:08:41.11
CTV.dial :air @10:08:41.13 delay 15 seconds @10:08:41.23 CS.seek 10ml @10:08:56.15
SSV.dial :spit,avoiding: :exhaust @10:08:57.57 CTV.dial CSV,avoiding: :intake @10:08:57.60
SS.reconfigure SSexhale @10:08:57.71 SS.seek 0ml @10:08:57.78 SSV.dial CBV,avoiding: :exhaust @10:09:05.04 CBV.dial between SSV and waste2 @10:09:05.47 CSV.dial between air and CTV @10:09:05.80 CS.seek 8.203ml @10:09:11.01 CTV.dial CSV,avoiding: :air @10:09:11.04
CSV.dial CTV @10:09:11.38 CS.reconfigure CSpressurize @10:09:11.42 CS.seek 0ml @10:09:33.05 CSV.dial :air @10:09:33.53 CS.reconfigure CSfast @10:09:33.58 CS.seek 10ml @10:09:56.95 CSV.dial between air and CTV @10:09:57.31 CS.seek 3.074ml @10:10:13.95 CTV.dial CSV,avoiding: :air @10:10:13.98
CSV.dial CTV @10:10:14.32 CS.reconfigure CSpressurize @10:10:14.36 CS.seek 0ml @10:10:22.17 BubblePoint: Intake @69.5psia, Outlet @14.1psia @10:10:22.18 CBV.dial SSV @10:10:22.53 SS.reconfigure SSconfig @10:10:22.58 SS.seek 10ml @10:10:27.92 delay 15 seconds @10:10:43.06 Evacuated: Intake @38.4psia, Outlet @16.6psia @10:10:43.07 SSV.dial :spit,avoiding: :exhaust @10:10:44.48 SS.reconfigure SSexhale @10:10:44.53 SS.seek 0ml @10:10:49.63 SS.reconfigure SSfast @10:10:49.68 SS.seek 5ml @10:10:52.37 SSV.dial CBV,avoiding: :exhaust @10:10:52.40
CBV.dial SSV @10:10:53.81 CTV.dial :air,avoiding: :intake @10:10:55.22 CSV.dial CTV @10:10:55.27
SS.seek 10ml @10:10:56.27 CSV.dial :air @10:10:56.77 CS.reconfigure CSfast @10:10:56.82 CS.seek 0ml @10:10:58.07
SSV.dial :spit,avoiding: :exhaust @10:10:58.63 CS.coast @10:10:59.47
SS.reconfigure SSexhale @10:10:59.52 SS.seek 0ml @10:11:04.76 SS.reconfigure SSfast @10:11:04.82 SS.seek 5ml @10:11:07.32 SSV.dial CBV,avoiding: :exhaust @10:11:07.35
CBV.dial SSV @10:11:09.77 SSV.dial :spit,avoiding: :exhaust @10:11:11.15 SS.reconfigure SSconfig @10:11:11.79 SS.seek 0ml @10:11:14.90 SS.coast @10:11:14.95 Lysing: Adding 1.5ml lysis @10:11:14.96 CBV.dial between CSV and waste1,avoiding: :DBV @10:11:16.53 CTV.dial CSV @10:11:17.92 CSV.dial CSR @10:11:18.75 Pulling 1ml of CSR.lysis @10:11:18.76 CSR.select :lysis @10:11:18.88 CS.reconfigure CSconfig @10:11:18.92 CS.seek 1ml @10:11:22.52 delay 5 seconds @10:11:27.55 CSR.select :air @10:11:27.73 CS.seek 6ml @10:11:41.42 delay 2 seconds @10:11:43.44 CSV.dial :waste1 @10:11:44.78 CS.reconfigure CSfast @10:11:44.83 CS.seek :empty @10:11:59.27 CSV.dial CSR @10:12:00.69 Pulling 1.5ml of CSR.lysis @10:12:00.70 CSR.select :lysis @10:12:00.82 CS.reconfigure CSconfig @10:12:00.87 CS.seek 1.5ml @10:12:05.67 delay 5 seconds @10:12:10.70 CSR.select :air @10:12:10.88 CS.seek 4.5ml @10:12:19.49 delay 2 seconds @10:12:21.51 CSV.dial CTV @10:12:22.00 CS.seek 1.275ml @10:12:31.28 Heating sh1 puck from tube 3 in Collection Clamp to 85C for 5:00 @10:12:31.30 CH.seek 85C,8:00 @10:15:39.89 CH.hold 5:00,85C @10:20:40.07 Collection Puck Heater at 85C after lysing @10:20:40.09 CS.seek 0ml @10:20:44.48 CTV.dial between spare and air,avoiding: :intake @10:20:46.31 CSV.dial :air @10:20:46.80 CS.seek 5ml @10:21:00.62 CSV.dial CTV @10:21:01.09 delay 2 seconds @10:21:03.11 CSV.dial :air @10:21:03.59 delay 2 seconds @10:21:05.61 CSV.dial CTV @10:21:06.09 delay 2 seconds @10:21:08.11 CSV.dial :air @10:21:08.59 delay 2 seconds @10:21:10.62 CS.seek 0ml @10:21:24.40 Recovering Lysate into Collection syringe @10:21:24.41 CSV.dial CBV @10:21:25.48 CBV.dial CSV @10:21:25.88 CS.seek 5ml @10:21:39.72 delay 5 seconds @10:21:44.75 CTV.dial :air @10:21:45.30 CS.seek 7ml @10:21:51.37 delay 5 seconds @10:21:56.41 CSV.dial :air2 @10:21:56.88 CS.seek 10ml @10:22:05.59 CSV.dial between air and CTV,avoiding: CBV @10:22:06.79 Lysing Complete @10:22:06.87 Storing sh1 puck in Collection Clamp to tube 2 @10:22:07.06 SC.reconfigure SCtight @10:22:07.11 SC.seek 2 @10:22:09.28 SC.reconfigure SCloose @10:22:09.50 Elbow.reconfigure TightElbow @10:22:09.55 Elbow.seek 2 @10:22:11.46 Forearm.reconfigure TightForearm @10:22:11.52 Forearm.seek 2 + 700 counts @10:22:12.57 SE.reconfigure SEpick @10:22:12.65 SE.seek beamBreak @10:22:15.46 SE.configure SEconfig @10:22:15.62 Elbow.reconfigure LimpElbow @10:22:15.67 Forearm.reconfigure LooseForearm @10:22:15.72 Forearm.seek 2 @10:22:16.00 Elbow.hold @10:22:16.07 Elbow.reconfigure TightElbow @10:22:16.18 SE.seek 2.07 Pucks below Top Plate @10:22:16.63 Forearm.force -20 @10:22:16.67 Forearm.seek 2 + 339 counts @10:22:16.99 Forearm.reconfigure TightForearm @10:22:17.04 Forearm.seek 2 + 700 counts @10:22:17.33 SE.seek 3.07 Pucks below Top Plate @10:22:17.88 Forearm.reconfigure LooseForearm @10:22:17.93 Forearm.seek :retract @10:22:18.89 Elbow.seek CC @10:22:20.54 Elbow.reconfigure LooseElbow @10:22:20.65 Forearm.seek CC @10:22:21.73 Hand.close @10:22:22.37 CC.seek :open @10:22:27.49 Forearm.seek :retract @10:22:28.53 Elbow.reconfigure TightElbow @10:22:28.58 Elbow.seek 2 @10:22:30.58 Elbow.reconfigure LimpElbow @10:22:30.64 Forearm.seek 2 @10:22:31.63 Elbow.hold @10:22:31.71 Elbow.reconfigure TightElbow @10:22:31.75 Hand.open @10:22:32.51 Forearm.force -20 @10:22:32.56 Forearm.seek 2 + 341 counts @10:22:32.87 Forearm.reconfigure TightForearm @10:22:32.91 Forearm.seek 2 + 700 counts @10:22:33.28 SE.seek :down @10:22:34.44
Forearm.reconfigure LooseForearm @10:22:34.49 Forearm.seek :retract @10:22:35.80 SE.coast @10:22:35.84
SC.coast @10:22:35.89 Tranferring lysate to PRV @10:22:35.90 CSV.dial :air @10:22:36.23 CS.seek 10ml @10:22:36.30 CSV.dial between air and CTV @10:22:36.64 AIV.dial :bypass @10:22:36.73 Beginning samplePassC @10:22:36.75 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:22:36.82 Hand.open @10:22:37.25 Elbow.seek FlushPuck::Garage @10:22:39.26 Elbow.reconfigure LooseElbow @10:22:39.38 Forearm.seek :garage @10:22:40.39 Hand.close @10:22:41.13 Forearm.seek :retract @10:22:42.12 Elbow.reconfigure TightElbow @10:22:42.17 Elbow.seek CC @10:22:45.14 Elbow.reconfigure LooseElbow @10:22:45.25 Forearm.reconfigure TightForearm @10:22:45.30 Forearm.seek CC @10:22:46.63 CC.seek :closed @10:22:50.90 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.05 @10:22:50.97 Hand.open @10:22:51.69 Forearm.reconfigure LooseForearm @10:22:51.73 Forearm.seek :retract @10:22:52.75 AWV.select :air @10:22:52.76 AIV.dial :bypass @10:22:52.77 PSV.dial PSR @10:22:54.82 Pulling 1.5ml of PSR.flush @10:22:54.83 PSR.select :flush @10:22:54.96 PS.seek 1.5ml @10:23:11.05 delay 5 seconds @10:23:16.08 PSV.dial :waste1 @10:23:16.57 PS.seek 0ml @10:23:32.61 PSV.dial PSR @10:23:33.09 PSR.select :air @10:23:33.22 PS.seek 2.5ml @10:23:59.34 PRV.dial CBV @10:23:59.36
CBV.dial PRV @10:24:00.23 PSV.dial :mixloop @10:24:01.28 CTV.dial :air,avoiding: :intake @10:24:01.29 PTV.dial :PRVmixing @10:24:01.38 PS.seek 0ml @10:24:27.45 PSV.dial :air @10:24:27.93 PS.seek 2.5ml @10:24:54.08 PSV.dial :mixloop @10:24:54.67 PS.seek 0ml @10:25:20.80 PSV.dial :air @10:25:21.26 PS.seek 2.5ml @10:25:47.36 PSV.dial :mixloop @10:25:47.82 PS.seek 0ml @10:26:14.02 Garaging FlushPuck in Collection Clamp @10:26:14.10 Elbow.reconfigure TightElbow @10:26:14.15 Elbow.seek CC @10:26:14.39 Elbow.reconfigure LooseElbow @10:26:14.50 Forearm.seek CC @10:26:15.53 Hand.close @10:26:16.13 CC.seek :open @10:26:20.51 Forearm.seek :retract @10:26:21.57 Elbow.reconfigure TightElbow @10:26:21.62 Elbow.seek :garage @10:26:24.81 Elbow.reconfigure LooseElbow @10:26:24.91 Forearm.reconfigure TightForearm @10:26:24.96 Forearm.seek :garage @10:26:26.14 Hand.open @10:26:26.82 Forearm.reconfigure LooseForearm @10:26:26.87 Forearm.seek :retract @10:26:27.89 Connecting PS to CS to transfer sample @10:26:27.90 CBV.dial AIV @10:26:27.91 AIV.dial :bypass @10:26:27.92 PSV.dial :mixloop @10:26:27.94 PTV.dial :PRVmixing @10:26:27.95 PRV.dial AIV @10:26:27.96 CTV.dial :air,avoiding: :intake @10:26:27.98 Loading lyfil puck from tube 3 into Collection Clamp @10:26:28.23 SC.reconfigure SCtight @10:26:28.28 SC.seek 3 @10:26:30.44 SC.reconfigure SCloose @10:26:30.66 Elbow.reconfigure TightElbow @10:26:30.71 Elbow.seek 3 @10:26:32.81 Forearm.reconfigure TightForearm @10:26:32.86 Forearm.seek 3 + 700 counts @10:26:33.89 SE.reconfigure SEpick @10:26:33.97 SE.seek beamBreak @10:26:35.44 SE.configure SEconfig @10:26:35.61 Elbow.seek 3 @10:26:35.82 Forearm.seek 3 + 300 counts @10:26:36.62 Forearm.reconfigure LooseForearm @10:26:36.75 Hand.close @10:26:37.40 SE.seek :down @10:26:37.64
Forearm.seek :retract @10:26:38.53 SE.coast @10:26:38.63
Elbow.seek CC @10:26:40.29 Elbow.reconfigure LooseElbow @10:26:40.39 Forearm.reconfigure TightForearm @10:26:40.44 Forearm.seek CC @10:26:41.80 CC.seek :closed @10:26:46.06 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.09 @10:26:46.07 Hand.open @10:26:46.70 Forearm.reconfigure LooseForearm @10:26:46.75 Forearm.seek :retract @10:26:47.75 SC.coast @10:26:47.79 CTV.dial CSV,avoiding: :intake @10:26:49.17 CSV.dial CTV @10:26:49.50 CS.seek 5ml @10:27:03.22 delay 1:20 @10:28:23.26 CS.seek 2.5ml @10:28:30.74 delay 40 seconds @10:29:10.79 CS.seek 0ml @10:29:18.35 delay 1:00 @10:30:18.38 PSV.dial :air @10:30:18.84 PS.seek 2.5ml @10:30:44.99 PSV.dial :mixloop @10:30:45.44 delay 3 seconds @10:30:48.46 PSV.dial :air @10:30:48.91 delay 2 seconds @10:30:50.93 PSV.dial :mixloop @10:30:51.40 delay 3 seconds @10:30:54.43 PSV.dial :waste1 @10:30:55.79 PS.seek :empty @10:31:21.99 CBV.dial :waste1 @10:31:22.49 delay 5 seconds @10:31:27.52 CBV.dial AIV @10:31:28.02 delay 10 seconds @10:31:38.05 CBV.dial :waste1 @10:31:38.54 delay 10 seconds @10:31:48.57 CBV.dial AIV @10:31:49.05 delay 10 seconds @10:31:59.08 CTV.dial CSV,avoiding: :intake @10:31:59.09 CBV.dial between AIV and waste1 @10:31:59.46 CSV.dial :air @10:32:00.06 CS.seek 10ml @10:32:26.54 CSV.dial CTV @10:32:27.00 CS.seek 0ml @10:32:53.54 CBV.dial :waste1 @10:32:53.87 delay 10 seconds @10:33:03.90 CTV.dial :air,avoiding: :intake @10:33:05.35 Storing lyfil puck in Collection Clamp to tube 2 @10:33:05.54 SC.reconfigure SCtight @10:33:05.58 SC.seek 2 @10:33:07.99 SC.reconfigure SCloose @10:33:08.21 Elbow.reconfigure TightElbow @10:33:08.26 Elbow.seek 2 @10:33:10.16 Forearm.reconfigure TightForearm @10:33:10.21 Forearm.seek 2 + 700 counts @10:33:11.23 SE.reconfigure SEpick @10:33:11.31 SE.seek beamBreak @10:33:14.70 SE.configure SEconfig @10:33:14.87 Elbow.reconfigure LimpElbow @10:33:14.93 Forearm.reconfigure LooseForearm @10:33:14.97 Forearm.seek 2 @10:33:15.29 Elbow.hold @10:33:15.36 Elbow.reconfigure TightElbow @10:33:15.47 SE.seek 3.08 Pucks below Top Plate @10:33:15.91 Forearm.force -20 @10:33:15.96 Forearm.seek 2 + 340 counts @10:33:16.28 Forearm.reconfigure TightForearm @10:33:16.33 Forearm.seek 2 + 700 counts @10:33:16.65 SE.seek 4.08 Pucks below Top Plate @10:33:17.24 Forearm.reconfigure LooseForearm @10:33:17.29 Forearm.seek :retract @10:33:18.26 Elbow.seek CC @10:33:19.92 Elbow.reconfigure LooseElbow @10:33:20.03 Forearm.seek CC @10:33:21.08 Hand.close @10:33:21.64 CC.seek :open @10:33:26.15 Forearm.seek :retract @10:33:27.17 Elbow.reconfigure TightElbow @10:33:27.22 Elbow.seek 2 @10:33:29.15 Elbow.reconfigure LimpElbow @10:33:29.20 Forearm.seek 2 @10:33:30.22 Elbow.hold @10:33:30.29 Elbow.reconfigure TightElbow @10:33:30.34 Hand.open @10:33:31.16 Forearm.force -20 @10:33:31.20 Forearm.seek 2 + 340 counts @10:33:31.50 Forearm.reconfigure TightForearm @10:33:31.56 Forearm.seek 2 + 700 counts @10:33:31.94 SE.seek :down @10:33:33.09
Forearm.reconfigure LooseForearm @10:33:33.14 Forearm.seek :retract @10:33:34.42 SE.coast @10:33:34.46
SC.coast @10:33:34.50 PSV.dial :air @10:33:35.61 PS.seek 0.499ml @10:33:41.49 delay 3 seconds @10:33:44.51 PSV.dial :mixloop @10:33:44.96 PRV.dial :air @10:33:45.45 delay 3 seconds @10:33:48.48 PRV.dial CBV @10:33:48.98 CBV.dial AIV @10:33:49.46 PRV.dial between CBV and air @10:33:49.82 PRV.dial :air @10:33:50.23 PS.reconfigure PSslow @10:33:50.28 PS.seek 0.599ml @10:33:52.81 PRV.dial :waste2 @10:33:53.57 PS.reconfigure PSconfig @10:33:53.62 PS.seek :empty @10:34:00.52 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:34:00.59 Hand.open @10:34:01.64 Elbow.seek FlushPuck::Garage @10:34:03.70 Elbow.reconfigure LooseElbow @10:34:03.81 Forearm.seek :garage @10:34:04.84 Hand.close @10:34:05.67 Forearm.seek :retract @10:34:06.70 Elbow.reconfigure TightElbow @10:34:06.75 Elbow.seek CC @10:34:09.69 Elbow.reconfigure LooseElbow @10:34:09.80 Forearm.reconfigure TightForearm @10:34:09.85 Forearm.seek CC @10:34:11.22 CC.seek :closed @10:34:15.48 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.04 @10:34:15.55 Hand.open @10:34:16.22 Forearm.reconfigure LooseForearm @10:34:16.27 Forearm.seek :retract @10:34:17.38 Clearing PML for modified lysate @10:34:17.39 CTV.dial :air,avoiding: :intake @10:34:17.40 PRV.dial :waste2 @10:34:17.42 PTV.dial :PRV,avoiding: :puck @10:34:18.20 delay 2 seconds @10:34:20.22 PTV.dial :PRVmixing,avoiding: :puck @10:34:21.04 delay 2 seconds @10:34:23.06 PSV.dial :PML @10:34:23.07 delay 2 seconds @10:34:25.10 PRV.dial :air @10:34:25.88 delay 2 seconds @10:34:27.96 PS.seek 1.499ml @10:34:43.93 delay 5 seconds @10:34:48.96 PS.seek 2.5ml @10:34:59.90 PSV.dial :air @10:35:00.38 PS.seek 0ml @10:35:26.71 mixing lysate with speDil @10:35:26.72 PSV.dial :PML @10:35:27.20 CBV.dial :PRV @10:35:27.21 PRV.dial :air @10:35:27.23 delay 2 seconds @10:35:29.25 PRV.dial :speDil @10:35:29.74 delay 2 seconds @10:35:31.83 PS.reconfigure PSslow1 @10:35:31.88 PS.seek 0.225ml @10:35:55.12 delay 5 seconds @10:36:00.15 PRV.dial CBV @10:36:00.95 delay 2 seconds @10:36:03.03 PS.seek 0.475ml @10:36:28.68 delay 5 seconds @10:36:33.71 PRV.dial :air @10:36:34.21 delay 2 seconds @10:36:36.24 PS.seek 0.965ml @10:37:26.15 delay 10 seconds @10:37:36.18 PRV.dial CBV @10:37:36.65 CTV.dial :air,avoiding: :intake @10:37:36.66 PSV.dial :air @10:37:37.14 PS.reconfigure PSconfig @10:37:37.19 PS.seek 2.5ml @10:37:53.59 delay 3 seconds @10:37:56.61 PSV.dial PTV @10:37:57.39 PTV.dial :PRV,avoiding: :puck @10:37:58.19 PS.seek 0ml @10:38:24.37 PSV.dial :air @10:38:25.76 PS.seek 2.5ml @10:38:51.87 delay 3 seconds @10:38:54.89 PSV.dial PTV @10:38:55.67 PTV.dial :PRV,avoiding: :puck @10:38:55.69 PS.seek 0ml @10:39:21.87 Pushing modified lysate into AIV Injection Loop @10:39:21.88 CBV.dial AIV @10:39:21.89 AIV.dial :bypass @10:39:21.91 PRV.dial CBV @10:39:21.92 PSV.dial :air @10:39:22.71 PS.seek 2.5ml @10:39:48.81 delay 3 seconds @10:39:51.83 PSV.dial :PML @10:39:52.31 PTV.dial :PRVmixing,avoiding: :puck @10:39:53.16 PS.reconfigure PSslow1 @10:39:53.21 PS.seek 1.74ml @10:41:10.78 delay 5 seconds @10:41:15.81 AIV.dial :sample @10:41:16.43 AWV.select :waste @10:41:16.60 PS.reconfigure PSslow2 @10:41:16.65 PS.seek 1.22ml @10:43:03.78 delay 15 seconds @10:43:18.81 AIV.dial :bypass @10:43:19.41 PS.reconfigure PSconfig @10:43:19.46 PS.seek 0ml @10:43:32.69 PSV.dial :air @10:43:33.18 PS.seek 2.5ml @10:43:59.25 PSV.dial :PML @10:43:59.73 PS.seek 0ml @10:44:25.85 AIV.dial :coilToMFB @10:44:25.86 AWV.select :air @10:44:26.03 Extracting Nucleic Acid from AIV @10:44:26.06
Beginning samplePassD @10:44:26.13 SPE.hold 2:46:40,55C @10:44:26.18 ASV.dial :SPEdelivery @10:44:26.27
AWV.select :air @10:44:26.28 AIV.dial :bypass @10:44:26.30 PSV.dial PSR @10:44:26.61 delay 20 seconds @10:44:27.45
Pulling 1.5ml of PSR.killMFB @10:44:27.46 PSR.select :killMFB @10:44:27.59 PS.seek 1.5ml @10:44:43.63 delay 5 seconds @10:44:46.64 primeLysateINT @10:44:46.65 AWV.select :air @10:44:46.66 AIV.dial :coilToMFB @10:44:46.68 ATV.dial AIV @10:44:48.44 AS.reconfigure ASslow1 @10:44:48.49 AS.seek 0.299ml @10:44:48.65
PSV.dial :waste1 @10:44:49.17 PS.seek 0ml @10:45:05.16 PSV.dial PSR @10:45:05.64 PSR.select :air @10:45:05.76 PS.seek 2.5ml @10:45:31.82 PRV.dial CBV @10:45:31.85
CBV.dial PRV @10:45:31.86 PSV.dial :mixloop @10:45:32.97 CTV.dial :air,avoiding: :intake @10:45:32.98 PTV.dial :PRVmixing @10:45:33.00 PS.seek 0ml @10:45:49.16 delay 10 seconds @10:45:59.10
PSV.dial PSR @10:45:59.18 ATV.dial :air2 @10:46:00.02 AS.seek 0.329ml @10:46:00.27
Pulling 1.5ml of PSR.flush @10:46:00.28 PSR.select :flush @10:46:00.41 PS.seek 1.5ml @10:46:06.34 ATV.dial :waste2 @10:46:06.89 AS.reconfigure ASnorm @10:46:06.94 AS.seek :empty @10:46:16.41
delay 5 seconds @10:46:21.44 PSV.dial :waste1 @10:46:21.95 PS.seek 0ml @10:46:23.91 Lysate primed! @10:46:23.92 AFV.select :holdingCoil @10:46:23.93 ATV.dial :air2 @10:46:24.55 AS.reconfigure ASslow1 @10:46:24.60 AS.seek 0.069ml @10:46:37.94
PSV.dial PSR @10:46:39.02 delay 3 seconds @10:46:39.06
PSR.select :air @10:46:39.19 PS.seek 2.5ml @10:46:42.05 ATV.dial :AIV @10:46:42.91 AS.seek 0.469ml @10:47:05.35 PRV.dial CBV @10:47:05.38
CBV.dial PRV @10:47:05.40 PSV.dial :mixloop @10:47:06.50 CTV.dial :air,avoiding: :intake @10:47:06.51 PTV.dial :PRVmixing @10:47:06.54 PS.seek 0ml @10:47:32.66 PSV.dial PSR @10:47:33.83 Pulling 1.5ml of PSR.flush @10:47:33.84 PSR.select :flush @10:47:33.96 PS.seek 1.5ml @10:47:49.91 delay 5 seconds @10:47:54.94 PSV.dial :waste1 @10:47:55.44 PS.seek 0ml @10:48:03.60 delay 5 seconds @10:48:08.69 Solid Phase Extraction Heater at 46.4C @10:48:08.70 ATV.dial ASV @10:48:09.10 ASV.dial :SPEdelivery @10:48:09.18 AS.seek 0.041ml @10:48:11.41
PSV.dial PSR @10:48:11.90 PSR.select :air @10:48:12.03 PS.seek 2.5ml @10:48:38.19 PRV.dial CBV @10:48:38.22
CBV.dial PRV @10:48:38.23 PSV.dial :mixloop @10:48:39.32 CTV.dial :air,avoiding: :intake @10:48:39.33 PTV.dial :PRVmixing @10:48:39.35 PS.seek 0ml @10:49:05.48 PSV.dial PSR @10:49:06.63 Pulling 1.5ml of PSR.flush @10:49:06.64 PSR.select :flush @10:49:06.77 PS.seek 1.5ml @10:49:22.79 delay 5 seconds @10:49:27.82 PSV.dial :waste1 @10:49:28.31 PS.seek 0ml @10:49:35.50 delay 10 seconds @10:49:44.32
PSV.dial PSR @10:49:44.80 PSR.select :air @10:49:44.93 PS.seek 2.5ml @10:49:45.52 rinseLysateLineINT @10:49:45.53 AFV.select :carrierSolution @10:49:45.64 delay 2 seconds @10:49:47.67 AS.reconfigure ASconfig @10:49:47.72 AS.seek 1ml @10:50:11.10 PRV.dial CBV @10:50:11.13
CBV.dial PRV @10:50:11.14 PSV.dial :mixloop @10:50:12.21 CTV.dial :air,avoiding: :intake @10:50:12.22 PTV.dial :PRVmixing @10:50:12.24 PS.reconfigure PSslow @10:50:12.29 PS.seek 1.2ml @10:50:39.10 PSV.dial :waste1 @10:50:40.48 PS.reconfigure PSconfig @10:50:40.53 PS.seek 0ml @10:50:43.14 delay 1 second @10:50:44.16 AFV.select :holdingCoil @10:50:44.28 delay 1 second @10:50:45.31 ATV.dial AIV @10:50:45.94 AIV.dial :coilToMFB @10:50:45.95 AWV.select :waste @10:50:46.07 AS.reconfigure ASslow0 @10:50:46.11 AS.seek 0ml @10:50:53.41
PSV.dial :air @10:50:54.49 PS.seek 2.5ml @10:51:20.70 PSV.dial :mixloop @10:51:21.16 PS.reconfigure PSslow @10:51:21.21 PS.seek 0ml @10:52:12.29 PSV.dial :air @10:52:12.76 PS.reconfigure PSconfig @10:52:12.81 PS.seek 2.5ml @10:52:26.97 ATV.dial :air2 @10:52:27.64 AS.reconfigure ASslow1 @10:52:27.69 AS.seek 0.05ml @10:52:38.04 ATV.dial :AIV @10:52:38.86 AS.seek 0.01ml @10:52:39.55
PSV.dial :mixloop @10:52:40.03 PS.reconfigure PSslow @10:52:40.07 PS.seek 0ml @10:52:47.19 ATV.dial :waste @10:52:48.78 AS.seek :empty @10:52:51.21 ethanol wash @10:52:51.22 ATV.dial :air2 @10:52:52.44 AS.reconfigure ASslow2 @10:52:52.49 AS.seek 0.009ml @10:53:02.85 delay 3 seconds @10:53:05.88 Pulling 0.4ml of Ethanol Wash @10:53:05.89 ATV.dial :ethanol @10:53:06.70 AS.reconfigure ASslow1 @10:53:06.75 AS.seek 0.409ml @10:53:31.26
Cleaning Collection Syringe @10:53:31.27 CSV.dial CSR @10:53:31.80 Pulling 4ml of CSR.flush @10:53:31.81 CSR.select :flush @10:53:31.94 CS.seek 4ml @10:53:43.10 delay 5 seconds @10:53:48.13 CSR.select :air @10:53:48.26 CS.seek 10ml @10:54:04.66 CSV.dial CTV @10:54:04.69
CTV.dial CSV @10:54:06.17 CBV.dial :waste1 @10:54:06.68 CS.seek 0ml @10:54:27.46 delay 3 seconds @10:54:30.49 ATV.dial :air1 @10:54:30.86 delay 3 seconds @10:54:33.20
CSV.dial CSR @10:54:33.73 Pulling 4ml of CSR.flush @10:54:33.74 CSR.select :flush @10:54:33.87 CS.seek 4ml @10:54:33.99 AS.seek 0.434ml @10:54:39.27 delay 3 seconds @10:54:42.30 ATV.dial :ethanol @10:54:42.89 AS.seek 0.419ml @10:54:45.03
delay 5 seconds @10:54:46.18 delay 3 seconds @10:54:49.21 ATV.dial ASV @10:54:50.05
CSR.select :air @10:54:50.18 CS.seek 10ml @10:54:50.48 AS.seek 0.092ml @10:55:06.53 CSV.dial CTV @10:55:06.57
CTV.dial CSV @10:55:07.05 CBV.dial :waste1 @10:55:07.07 CS.seek 0ml @10:55:33.73 Garaging FlushPuck in Collection Clamp @10:55:33.80 Elbow.reconfigure TightElbow @10:55:33.85 Elbow.seek CC @10:55:34.11 Elbow.reconfigure LooseElbow @10:55:34.22 Forearm.seek CC @10:55:35.22 Hand.close @10:55:35.80 CC.seek :open @10:55:40.13 Forearm.seek :retract @10:55:41.16 Elbow.reconfigure TightElbow @10:55:41.21 Elbow.seek :garage @10:55:44.35 Elbow.reconfigure LooseElbow @10:55:44.46 Forearm.reconfigure TightForearm @10:55:44.51 Forearm.seek :garage @10:55:45.65 Hand.open @10:55:46.37 Forearm.reconfigure LooseForearm @10:55:46.42 Forearm.seek :retract @10:55:47.44 Whole Cell Archive sampling 1500ml @10:55:47.46 Loading wcr puck from tube 3 into Collection Clamp @10:55:47.72 SC.reconfigure SCtight @10:55:47.77 SC.seek 3 @10:55:49.91 SC.reconfigure SCloose @10:55:50.80 Elbow.reconfigure TightElbow @10:55:50.85 Elbow.seek 3 @10:55:53.02 Forearm.reconfigure TightForearm @10:55:53.07 Forearm.seek 3 + 700 counts @10:55:54.14 SE.reconfigure SEpick @10:55:54.22 SE.seek beamBreak @10:55:55.75 SE.configure SEconfig @10:55:55.93 Elbow.seek 3 @10:55:56.19 Forearm.seek 3 + 300 counts @10:55:56.43 Forearm.reconfigure LooseForearm @10:55:56.50 Hand.close @10:55:56.53 delay 3 seconds @10:55:57.20 SE.seek :down @10:55:57.44 Forearm.seek :retract @10:55:58.40 Elbow.seek CC @10:55:58.55 SE.coast @10:55:59.56 ATV.dial :waste2 @10:56:00.03 Elbow.reconfigure LooseElbow @10:56:00.14 Forearm.reconfigure TightForearm @10:56:00.20 Forearm.seek CC @10:56:00.29 AS.reconfigure ASconfig @10:56:00.34 AS.seek :empty @10:56:01.53 CC.seek :closed @10:56:05.78 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.04 @10:56:05.80 Hand.open @10:56:05.99 ATV.dial :air2 @10:56:06.39 AS.reconfigure ASslow1 @10:56:06.45 AS.seek 1ml @10:56:06.59 Forearm.reconfigure LooseForearm @10:56:06.65 Forearm.seek :retract @10:56:07.69 SC.coast @10:56:07.73 collectSample 1500ml w/bubble point of 55psi @10:56:07.75 Priming 7ml @10:56:07.76 Intake.select :close @10:56:07.77 Exhaust.select :close @10:56:07.78 SS.hold @10:56:08.01 Calibrating pressure sensors @10:56:08.02 CTV.dial :air @10:56:09.43 SSV.dial :spit,avoiding: :exhaust @10:56:09.45 SS.reconfigure SSfast @10:56:09.50 SS.seek 5ml @10:56:12.13 SSV.dial CBV @10:56:12.16 CBV.dial SSV @10:56:13.68 delay 3.8 seconds @10:56:17.63 SSV.dial :spit @10:56:19.02 SS.seek 0ml @10:56:21.63 SSV.dial CBV @10:56:23.04 CTV.dial :intake @10:56:24.50 SSV.dial CBV @10:56:24.52 CBV.dial SSV @10:56:24.55 Allowing 40s for 7.0ml of remaining 7.0ml @10:56:24.56 Intake.select :open @10:56:26.73 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:56:26.75 SS.reconfigure SSinhale.with maxSpeed: 150 @10:56:26.81 SS.seek 7ml @10:56:31.08 Vacuum too high -- waiting up to 40s @10:56:35.29 SS.reconfigure SSinhale.with maxSpeed: 135 @10:56:35.34 SS.seek 7ml @10:56:37.60 Stroke completed -- waiting for pressure to equalize ... @10:56:43.39 Intake.select :close @10:56:43.55 SSV.dial :exhaust @10:56:44.37 Exhaust.select :open @10:56:44.49 SS.reconfigure SSexhale @10:56:44.54 SS.seek 0ml @10:56:48.14 Exhaust.select :close @10:56:48.25 Sampling 1500.0ml @10:56:48.27 Intake.select :close @10:56:48.28 Exhaust.select :close @10:56:48.29 SS.reconfigure SSconfig @10:56:48.34 SS.hold @10:56:48.57 CTV.dial :intake @10:56:48.59 SSV.dial CBV @10:56:48.62 CBV.dial SSV @10:56:49.43 Allowing 130s for 25.0ml of remaining 1500.0ml @10:56:49.44 Intake.select :open @10:56:51.63 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:56:51.64 SS.reconfigure SSinhale.with maxSpeed: 135 @10:56:51.69 SS.seek 25ml @10:56:55.86 Vacuum too high -- waiting up to 40s @10:56:58.70 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:56:58.72 SS.reconfigure SSinhale.with maxSpeed: 122 @10:56:59.43 SS.seek 25ml @10:57:02.52 Vacuum too high -- waiting up to 40s @10:57:05.19 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:57:05.21 SS.reconfigure SSinhale.with maxSpeed: 110 @10:57:05.25 SS.seek 25ml @10:57:07.98 Vacuum too high -- waiting up to 40s @10:57:10.63 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:57:10.65 SS.reconfigure SSinhale.with maxSpeed: 99 @10:57:10.70 SS.seek 25ml @10:57:13.61 Vacuum too high -- waiting up to 40s @10:57:16.24 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:57:16.26 SS.reconfigure SSinhale.with maxSpeed: 89 @10:57:16.31 SS.seek 25ml @10:57:18.90 Vacuum too high -- waiting up to 40s @10:57:21.50 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:57:21.52 SS.reconfigure SSinhale.with maxSpeed: 80 @10:57:21.57 SS.seek 25ml @10:57:24.43 Vacuum too high -- waiting up to 40s @10:57:27.21 SS.reconfigure SSinhale.with maxSpeed: 72 @10:57:27.27 SS.seek 25ml @10:57:29.81 Vacuum too high -- waiting up to 40s @10:57:32.47 SS.reconfigure SSinhale.with maxSpeed: 65 @10:57:32.52 SS.seek 25ml @10:57:35.18 Vacuum too high -- waiting up to 40s @10:57:38.06 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:57:38.08 SS.reconfigure SSinhale.with maxSpeed: 59 @10:57:38.13 SS.seek 25ml @10:57:42.27 Vacuum too high -- waiting up to 40s @10:57:45.12 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:57:45.14 SS.reconfigure SSinhale.with maxSpeed: 53 @10:57:45.19 SS.seek 25ml @10:57:46.11 Stroke completed -- waiting for pressure to equalize ... @10:57:51.15 Intake.select :close @10:57:51.31 SSV.dial :exhaust @10:57:52.14 Exhaust.select :open @10:57:52.26 SS.reconfigure SSexhale @10:57:52.32 SS.seek 0ml @10:58:01.31 Exhaust.select :close @10:58:01.43 SSV.dial CBV @10:58:01.45 CBV.dial SSV @10:58:02.28 Allowing 130s for 25.0ml of remaining 1475.0ml @10:58:02.29 Intake.select :open @10:58:05.10 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:58:05.14 SS.reconfigure SSinhale.with maxSpeed: 53 @10:58:05.19 SS.seek 25ml @10:58:11.05 Vacuum too high -- waiting up to 40s @10:58:13.45 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:58:13.47 SS.reconfigure SSinhale.with maxSpeed: 48 @10:58:13.52 SS.seek 25ml @10:58:17.83 Vacuum too high -- waiting up to 40s @10:58:20.22 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:58:20.24 SS.reconfigure SSinhale.with maxSpeed: 43 @10:58:20.28 SS.seek 25ml @10:58:24.91 Vacuum too high -- waiting up to 40s @10:58:27.53 SS.reconfigure SSinhale.with maxSpeed: 39 @10:58:27.58 SS.seek 25ml @10:58:34.05 Vacuum too high -- waiting up to 40s @10:58:36.38 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:58:36.40 SS.reconfigure SSinhale.with maxSpeed: 35 @10:58:36.48 SS.seek 25ml @10:58:44.79 Vacuum too high -- waiting up to 40s @10:58:48.07 SS.reconfigure SSinhale.with maxSpeed: 32 @10:58:48.12 SS.seek 25ml @10:59:00.08 Vacuum too high -- waiting up to 40s @10:59:02.75 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:59:02.77 SS.reconfigure SSinhale.with maxSpeed: 29 @10:59:02.81 SS.seek 25ml @10:59:12.15 Stroke completed -- waiting for pressure to equalize ... @10:59:19.83 Intake.select :close @10:59:19.99 SSV.dial :exhaust @10:59:20.81 Exhaust.select :open @10:59:20.93 SS.reconfigure SSexhale @10:59:20.98 SS.seek 0ml @10:59:28.01 delay 5 seconds @10:59:30.24 Exhaust.select :close @10:59:30.36 SSV.dial CBV @10:59:30.38 CBV.dial SSV @10:59:31.21 Allowing 130s for 25.0ml of remaining 1450.0ml @10:59:31.22 Intake.select :open @10:59:33.03 ATV.dial ASV @10:59:33.41 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:59:33.42 SS.reconfigure SSinhale.with maxSpeed: 29 @10:59:33.47 SS.seek 25ml @10:59:33.99 ASV.dial :SPEdelivery @10:59:34.01 AS.seek 0.02ml @11:00:00.91 Vacuum too high -- waiting up to 40s @11:00:03.52 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:00:03.54 SS.reconfigure SSinhale.with maxSpeed: 26 @11:00:03.59 SS.seek 25ml @11:00:41.00 Stroke completed -- waiting for pressure to equalize ... @11:00:49.31 Intake.select :close @11:00:49.47 SSV.dial :exhaust @11:00:50.29 Exhaust.select :open @11:00:50.41 SS.reconfigure SSexhale @11:00:50.46 SS.seek 0ml @11:00:59.46 Exhaust.select :close @11:00:59.58 SSV.dial CBV @11:00:59.60 CBV.dial SSV @11:01:00.43 Allowing 130s for 25.0ml of remaining 1425.0ml @11:01:00.44 Intake.select :open @11:01:02.63 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:01:02.65 SS.reconfigure SSinhale.with maxSpeed: 26 @11:01:02.69 SS.seek 25ml @11:02:10.40 Stroke completed -- waiting for pressure to equalize ... @11:02:19.32 Intake.select :close @11:02:19.48 SSV.dial :exhaust @11:02:20.31 Exhaust.select :open @11:02:20.43 SS.reconfigure SSexhale @11:02:20.49 SS.seek 0ml @11:02:29.75 Exhaust.select :close @11:02:29.87 SSV.dial CBV @11:02:29.89 CBV.dial SSV @11:02:30.73 Allowing 130s for 25.0ml of remaining 1400.0ml @11:02:30.74 Intake.select :open @11:02:32.92 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:02:32.94 SS.reconfigure SSinhale.with maxSpeed: 26 @11:02:32.99 SS.seek 25ml @11:02:51.38 delay 5 seconds @11:02:56.41 ATV.dial :air2 @11:02:57.42 AS.seek 0.52ml @11:03:40.70 Stroke completed -- waiting for pressure to equalize ... @11:03:50.25 Intake.select :close @11:03:51.09 SSV.dial :exhaust @11:03:51.90 Exhaust.select :open @11:03:52.02 SS.reconfigure SSexhale @11:03:52.07 SS.seek 0ml @11:04:01.32 Exhaust.select :close @11:04:01.44 SSV.dial CBV @11:04:01.46 CBV.dial SSV @11:04:02.28 Allowing 130s for 25.0ml of remaining 1375.0ml @11:04:02.29 Intake.select :open @11:04:04.48 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:04:04.50 SS.reconfigure SSinhale.with maxSpeed: 26 @11:04:04.54 SS.seek 25ml @11:04:38.28 delay 5 seconds @11:04:43.31 ATV.dial ASV @11:04:44.26 ASV.dial :wasteR @11:04:44.65 AS.seek 0.3ml @11:04:50.36 Vacuum too high -- waiting up to 40s @11:04:53.02 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:04:53.03 SS.reconfigure SSinhale.with maxSpeed: 23 @11:04:53.08 SS.seek 25ml @11:05:18.11 Stroke completed -- waiting for pressure to equalize ... @11:05:27.06 Intake.select :close @11:05:27.22 SSV.dial :exhaust @11:05:28.03 Exhaust.select :open @11:05:28.15 SS.reconfigure SSexhale @11:05:28.21 SS.seek 0ml @11:05:29.17 delay 20 seconds @11:05:37.61 Exhaust.select :close @11:05:37.73 SSV.dial CBV @11:05:37.75 CBV.dial SSV @11:05:38.59 Allowing 130s for 25.0ml of remaining 1350.0ml @11:05:38.60 Intake.select :open @11:05:40.78 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:05:40.80 SS.reconfigure SSinhale.with maxSpeed: 23 @11:05:40.85 SS.seek 25ml @11:05:49.21 AS.seek 0.07ml @11:06:35.71 delay 20 seconds @11:06:55.74 ATV.dial :waste2 @11:06:56.48 AS.reconfigure ASconfig @11:06:56.53 AS.seek :empty @11:06:56.91 Stroke completed -- waiting for pressure to equalize ... @11:07:00.93 Elution @11:07:00.94 ATV.dial :air1 @11:07:02.68 AS.reconfigure ASslow1 @11:07:02.73 AS.seek 0.049ml @11:07:07.54 Intake.select :close @11:07:07.70 SSV.dial :exhaust @11:07:08.53 Exhaust.select :open @11:07:08.65 SS.reconfigure SSexhale @11:07:08.70 SS.seek 0ml @11:07:13.09 delay 3 seconds @11:07:16.12 ATV.dial :ElutionWater @11:07:16.79 AS.seek 0.109ml @11:07:17.75 Exhaust.select :close @11:07:17.87 SSV.dial CBV @11:07:17.89 CBV.dial SSV @11:07:18.73 Allowing 130s for 25.0ml of remaining 1325.0ml @11:07:18.74 Intake.select :open @11:07:20.93 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:07:20.95 SS.reconfigure SSinhale.with maxSpeed: 23 @11:07:20.99 SS.seek 25ml @11:07:29.14 delay 3 seconds @11:07:32.17 ATV.dial ASV,via: :air2 @11:07:34.18 ASV.dial :SPEelution @11:07:34.76 AFV.select :carrierSolution @11:07:34.92 AS.reconfigure ASnorm @11:07:34.97 AS.seek 0.759ml @11:08:08.11 delay 3 seconds @11:08:09.77 Vacuum too high -- waiting up to 40s @11:08:11.13 AFV.select :holdingCoil @11:08:11.38 AS.reconfigure ASslow1 @11:08:11.43 AS.seek 0.474ml @11:08:12.74 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:08:12.76 SS.reconfigure SSinhale.with maxSpeed: 21 @11:08:12.80 SS.seek 25ml @11:08:43.76 Stroke completed -- waiting for pressure to equalize ... @11:08:54.66 Intake.select :close @11:08:54.82 SSV.dial :exhaust @11:08:55.64 Exhaust.select :open @11:08:55.76 SS.reconfigure SSexhale @11:08:55.81 SS.seek 0ml @11:09:04.92 Exhaust.select :close @11:09:05.04 SSV.dial CBV @11:09:05.06 CBV.dial SSV @11:09:05.90 Allowing 130s for 25.0ml of remaining 1300.0ml @11:09:05.91 Intake.select :open @11:09:08.10 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:09:08.12 SS.reconfigure SSinhale.with maxSpeed: 21 @11:09:08.16 SS.seek 25ml @11:09:08.98 delay 5 seconds @11:09:14.07 AS.reconfigure ASslow2 @11:09:14.12 AS.seek 0.439ml @11:09:49.61 delay 1:00 @11:10:10.02 Vacuum too high -- waiting up to 40s @11:10:13.47 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:10:13.49 SS.reconfigure SSinhale.with maxSpeed: 19 @11:10:13.53 SS.seek 25ml @11:10:38.56 Stroke completed -- waiting for pressure to equalize ... @11:10:49.63 pushing eluate to holding coil @11:10:49.70 AS.seek 0.279ml @11:10:49.98 Intake.select :close @11:10:50.14 SSV.dial :exhaust @11:10:50.97 Exhaust.select :open @11:10:51.09 SS.reconfigure SSexhale @11:10:51.14 SS.seek 0ml @11:11:00.15 Exhaust.select :close @11:11:00.27 SSV.dial CBV @11:11:00.29 CBV.dial SSV @11:11:01.14 Allowing 130s for 25.0ml of remaining 1275.0ml @11:11:01.15 Intake.select :open @11:11:03.34 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:11:03.35 SS.reconfigure SSinhale.with maxSpeed: 19 @11:11:03.40 SS.seek 25ml @11:12:17.11 Vacuum too high -- waiting up to 40s @11:12:20.70 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:12:20.72 SS.reconfigure SSinhale.with maxSpeed: 17 @11:12:20.76 SS.seek 25ml @11:12:42.67 Stroke completed -- waiting for pressure to equalize ... @11:12:54.75 Intake.select :close @11:12:54.91 SSV.dial :exhaust @11:12:55.72 Exhaust.select :open @11:12:55.84 SS.reconfigure SSexhale @11:12:55.89 SS.seek 0ml @11:13:05.37 Exhaust.select :close @11:13:05.49 SSV.dial CBV @11:13:05.51 CBV.dial SSV @11:13:06.33 Allowing 130s for 25.0ml of remaining 1250.0ml @11:13:06.34 Intake.select :open @11:13:08.53 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:13:08.55 SS.reconfigure SSinhale.with maxSpeed: 17 @11:13:08.59 SS.seek 25ml @11:13:30.69 delay 30 seconds @11:14:00.72 ATV.dial :waste,via: :air3 @11:14:02.36 AS.reconfigure ASconfig @11:14:02.41 AS.seek :empty @11:14:18.80 Parking Bleach in LysateSource line and SPEcolumn @11:14:18.81 ATV.dial :air3 @11:14:19.62 AS.reconfigure ASslow1 @11:14:19.67 AS.seek 0.029ml @11:14:26.03 ATV.dial :ElutionWater @11:14:26.62 AS.seek 0.059ml @11:14:29.29 Vacuum too high -- waiting up to 40s @11:14:32.89 ATV.dial :air3 @11:14:33.12 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:14:33.15 SS.reconfigure SSinhale.with maxSpeed: 15 @11:14:33.19 SS.seek 25ml @11:14:33.48 AS.seek 0.069ml @11:14:35.75 delay 2 seconds @11:14:37.77 ATV.dial :bleach @11:14:38.24 AS.seek 0.769ml @11:14:59.39 Stroke completed -- waiting for pressure to equalize ... @11:15:12.79 Intake.select :close @11:15:12.95 SSV.dial :exhaust @11:15:13.76 Exhaust.select :open @11:15:13.88 SS.reconfigure SSexhale @11:15:13.93 SS.seek 0ml @11:15:23.40 Exhaust.select :close @11:15:23.53 SSV.dial CBV @11:15:23.55 CBV.dial SSV @11:15:24.39 Allowing 130s for 25.0ml of remaining 1225.0ml @11:15:24.40 Intake.select :open @11:15:26.59 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:15:26.61 SS.reconfigure SSinhale.with maxSpeed: 15 @11:15:26.65 SS.seek 25ml @11:16:48.83 Vacuum too high -- waiting up to 40s @11:16:52.95 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:16:53.54 SS.reconfigure SSinhale.with maxSpeed: 14 @11:16:53.69 SS.seek 25ml @11:16:59.27 delay 3 seconds @11:17:02.30 ATV.dial :air3 @11:17:02.83 delay 3 seconds @11:17:05.92 AS.seek 0.794ml @11:17:11.21 delay 3 seconds @11:17:14.24 ATV.dial :bleach @11:17:14.85 AS.seek 0.779ml @11:17:18.14 delay 3 seconds @11:17:21.17 ATV.dial :AIV @11:17:22.38 AS.reconfigure ASconfig @11:17:22.44 AS.seek 0.015ml @11:17:31.75 Stroke completed -- waiting for pressure to equalize ... @11:17:34.41 SS.reconfigure SSconfig @11:17:34.46 SS.hold @11:17:34.59 Intake.select :close @11:17:34.76 ShallowSampler::Clogged in WCR -- progress too slow after 300.0ml @11:17:34.78 SSV.dial :exhaust @11:17:35.59 Exhaust.select :open @11:17:35.71 SS.reconfigure SSexhale @11:17:35.76 SS.seek 0ml @11:17:45.41 Exhaust.select :close @11:17:45.52 Sampled 300.0ml @11:17:45.54 Drying puck before fixing for 4:00 @11:17:45.55 CBV.dial :waste2b @11:17:46.37 delay 1:00 @11:18:06.66 ATV.dial :waste @11:18:08.08 AS.seek :empty @11:18:09.30 ATV.dial :air3 @11:18:09.96 AS.reconfigure ASslow1 @11:18:10.01 AS.seek 0.009ml @11:18:12.29 delay 2 seconds @11:18:14.31 ATV.dial :bleach @11:18:14.76 AS.seek 0.579ml @11:18:46.40 CSV.dial :air @11:18:46.88 CS.reconfigure CSfast @11:18:46.93 CS.seek 10ml @11:19:11.03 Pumping wcr puck from tube 3 up to 55psi... @11:19:11.04 CBV.dial SSV @11:19:12.42 CBV.dial between CSV and waste2b @11:19:13.46 CSV.dial between air and CTV @11:19:13.84 CS.seek 7.149ml @11:19:21.45 CTV.dial CSV,avoiding: :air @11:19:21.47 CSV.dial CTV @11:19:22.33 CS.reconfigure CSpressurize @11:19:22.39 CS.seek 0ml @11:19:41.25 CSV.dial :air @11:19:41.75 CS.reconfigure CSfast @11:19:41.81 CS.seek 10ml @11:20:05.87 CSV.dial between air and CTV @11:20:06.24 CS.seek 2.522ml @11:20:09.66 delay 3 seconds @11:20:12.68 ATV.dial :air3 @11:20:13.22 delay 3 seconds @11:20:16.31 AS.seek 0.604ml @11:20:21.61 delay 3 seconds @11:20:24.60 CTV.dial CSV,avoiding: :air @11:20:24.62 CSV.dial CTV @11:20:24.69 ATV.dial :bleach @11:20:24.96 CS.reconfigure CSpressurize @11:20:25.01 CS.seek 0ml @11:20:25.82 AS.seek 0.589ml @11:20:29.11 delay 3 seconds @11:20:30.08 CBV.dial :waste2b @11:20:30.44 CS.reconfigure CSconfig @11:20:30.49 CS.seek :empty @11:20:32.13 ATV.dial :ASV @11:20:33.13 ASV.dial :wasteR @11:20:33.72 AS.seek 0ml @11:20:34.03 delay 1:00 @11:21:34.06 CTV.dial :air,avoiding: :intake @11:21:35.45 Fix #1 of 2 (with rnal) for 2:00 @11:21:35.47 CSV.dial CSR @11:21:36.01 Pulling 2ml of CSR.rnal @11:21:36.02 CSR.select :rnal @11:21:36.14 CS.seek 2ml @11:21:42.28 delay 5 seconds @11:21:47.31 CSR.select :air @11:21:47.44 CS.reconfigure CSfast @11:21:47.49 CS.seek 10ml @11:22:06.78 Pumping wcr puck from tube 3 up to 55psi... @11:22:06.80 CBV.dial SSV @11:22:08.18 CBV.dial between CSV and waste2b @11:22:09.21 CSV.dial between air and CTV @11:22:09.86 CS.seek 7.227ml @11:22:17.29 CTV.dial CSV,avoiding: :air @11:22:17.31 CSV.dial CTV @11:22:18.83 CS.reconfigure CSpressurize @11:22:18.88 CS.seek 0ml @11:22:32.47 delay 1:00 @11:22:38.01 CSV.dial :air @11:22:38.51 CS.reconfigure CSfast @11:22:38.56 CS.seek 10ml @11:23:02.73 CSV.dial between air and CTV @11:23:03.08 CS.seek 2.153ml @11:23:22.20 CTV.dial CSV,avoiding: :air @11:23:22.22 CSV.dial CTV @11:23:22.58 CS.reconfigure CSpressurize @11:23:22.63 CS.seek 0ml @11:23:24.49 delay 2:00 @11:23:32.50 AFV.select :carrierSolution @11:23:32.60 delay 2 seconds @11:23:34.63 AS.reconfigure ASconfig @11:23:34.68 AS.seek 1ml @11:24:32.46 delay 1 second @11:24:33.48 AFV.select :holdingCoil @11:24:33.58 delay 1 second @11:24:34.61 ATV.dial ASV @11:24:34.62 ASV.dial :wasteR @11:24:34.64 AS.reconfigure ASslow1 @11:24:34.68 AS.seek 0ml @11:25:24.52 CBV.dial :waste2b @11:25:24.86 CS.reconfigure CSconfig @11:25:24.91 CS.seek :empty @11:25:30.14 delay 1:00 @11:26:30.17 CTV.dial :air,avoiding: :intake @11:26:31.57 Fix #2 of 2 (with rnal) for 2:00 @11:26:31.59 CSV.dial CSR @11:26:32.11 Pulling 2ml of CSR.rnal @11:26:32.13 CSR.select :rnal @11:26:32.25 CS.seek 2ml @11:26:38.32 delay 5 seconds @11:26:43.35 CSR.select :air @11:26:43.48 CS.reconfigure CSfast @11:26:43.53 CS.seek 10ml @11:27:02.88 Pumping wcr puck from tube 3 up to 55psi... @11:27:02.90 CBV.dial SSV @11:27:04.88 CBV.dial between CSV and waste2b @11:27:05.94 CSV.dial between air and CTV @11:27:06.60 CS.seek 7.196ml @11:27:14.05 CTV.dial CSV,avoiding: :air @11:27:14.07 CSV.dial CTV @11:27:15.55 CS.reconfigure CSpressurize @11:27:15.60 CS.seek 0ml @11:27:34.66 CSV.dial :air @11:27:35.17 CS.reconfigure CSfast @11:27:35.22 CS.seek 10ml @11:27:56.05 delay 20 seconds @11:27:59.11 CSV.dial between air and CTV @11:27:59.46 CS.seek 2.169ml @11:28:16.08 ATV.dial :air3 @11:28:17.51 AS.seek 0.59ml @11:28:18.74 CTV.dial CSV,avoiding: :air @11:28:18.76 CSV.dial CTV @11:28:19.11 CS.reconfigure CSpressurize @11:28:19.17 CS.seek 0ml @11:28:21.09 delay 2:00 @11:30:16.46 delay 3 seconds @11:30:19.49 ATV.dial :ASV @11:30:20.82 ASV.dial :wasteR @11:30:20.90 AS.seek 0.09ml @11:30:21.13 CBV.dial :waste2b @11:30:21.49 CS.reconfigure CSconfig @11:30:21.55 CS.seek :empty @11:30:26.65 delay 1:00 @11:31:26.69 CTV.dial :air,avoiding: :intake @11:31:28.18 Storing wcr puck in Collection Clamp to tube 2 @11:31:28.37 SC.reconfigure SCtight @11:31:28.42 SC.seek 2 @11:31:30.61 SC.reconfigure SCloose @11:31:30.84 Elbow.reconfigure TightElbow @11:31:30.90 Elbow.seek 2 @11:31:32.77 Forearm.reconfigure TightForearm @11:31:32.82 Forearm.seek 2 + 700 counts @11:31:33.85 SE.reconfigure SEpick @11:31:33.93 SE.seek beamBreak @11:31:36.59 SE.configure SEconfig @11:31:36.76 Elbow.reconfigure LimpElbow @11:31:36.81 Forearm.reconfigure LooseForearm @11:31:36.86 Forearm.seek 2 @11:31:37.18 Elbow.hold @11:31:37.25 Elbow.reconfigure TightElbow @11:31:37.36 SE.seek 4.07 Pucks below Top Plate @11:31:37.86 Forearm.force -20 @11:31:37.90 Forearm.seek 2 + 326 counts @11:31:38.83 Forearm.reconfigure TightForearm @11:31:38.92 Forearm.seek 2 + 700 counts @11:31:39.26 SE.seek 5.07 Pucks below Top Plate @11:31:39.88 Forearm.reconfigure LooseForearm @11:31:39.93 Forearm.seek :retract @11:31:40.86 Elbow.seek CC @11:31:42.59 Elbow.reconfigure LooseElbow @11:31:42.70 Forearm.seek CC @11:31:43.75 Hand.close @11:31:44.36 CC.seek :open @11:31:48.90 Forearm.seek :retract @11:31:49.91 Elbow.reconfigure TightElbow @11:31:49.96 Elbow.seek 2 @11:31:51.91 Elbow.reconfigure LimpElbow @11:31:51.97 Forearm.seek 2 @11:31:52.99 Elbow.hold @11:31:53.07 Elbow.reconfigure TightElbow @11:31:53.12 Hand.open @11:31:53.88 Forearm.force -20 @11:31:53.93 Forearm.seek 2 + 342 counts @11:31:54.25 Forearm.reconfigure TightForearm @11:31:54.30 Forearm.seek 2 + 700 counts @11:31:54.72 SE.seek :down @11:31:55.88 Forearm.reconfigure LooseForearm @11:31:55.93 Forearm.seek :retract @11:31:57.05 SE.coast @11:31:57.09 SC.coast @11:31:57.13 Starting finalPurge @11:31:57.14 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:31:57.22 Hand.open @11:31:57.63 Elbow.seek FlushPuck::Garage @11:31:59.65 Elbow.reconfigure LooseElbow @11:31:59.76 Forearm.seek :garage @11:32:00.77 Hand.close @11:32:01.49 Forearm.seek :retract @11:32:01.79 delay 30 seconds @11:32:02.50
Elbow.reconfigure TightElbow @11:32:02.55 Elbow.seek CC @11:32:05.51 Elbow.reconfigure LooseElbow @11:32:05.62 Forearm.reconfigure TightForearm @11:32:05.67 Forearm.seek CC @11:32:07.00 CC.seek :closed @11:32:12.01 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.04 @11:32:12.09 Hand.open @11:32:12.81 Forearm.reconfigure LooseForearm @11:32:12.86 Forearm.seek :retract @11:32:13.93 Flushing the Collection Valve::Manifold @11:32:14.08 CSV.dial CSR @11:32:14.61 Pulling 5ml of CSR.flush @11:32:14.62 CSR.select :flush @11:32:14.75 CS.seek 5ml @11:32:28.48 delay 5 seconds @11:32:31.82 ATV.dial :extSrc @11:32:32.55 delay 3 seconds @11:32:33.50
CSR.select :air @11:32:33.67 CS.reconfigure CSfast @11:32:33.72 CS.seek 7.5ml @11:32:35.58 ATV.dial :ElutionWater @11:32:36.66 AS.seek 0.12ml @11:32:40.55
CSV.dial :waste @11:32:41.92 CS.reconfigure CSconfig @11:32:41.97 CS.seek 0ml @11:32:42.96 ATV.dial :air3 @11:32:43.56 AS.seek 0.13ml @11:32:45.93 delay 2 seconds @11:32:47.95 ATV.dial :bleach @11:32:48.39 AS.seek 0.23ml @11:33:02.17
Flushing lower manifold's waste1 port @11:33:02.31 CSV.dial :flush @11:33:02.78 CS.reconfigure CSfast @11:33:02.84 CS.seek 5ml @11:33:08.79 delay 3 seconds @11:33:11.82 ATV.dial :air3 @11:33:12.35 delay 3 seconds @11:33:15.36
delay 5 seconds @11:33:15.44 AS.seek 0.255ml @11:33:20.38
CSV.dial :air @11:33:20.73 delay 3 seconds @11:33:20.94
CS.seek 7ml @11:33:23.76 ATV.dial :bleach @11:33:24.38 AS.seek 0.24ml @11:33:26.52 CSV.dial CTV @11:33:26.56
CTV.dial CSV @11:33:27.65 delay 3 seconds @11:33:28.02
CBV.dial :waste1 @11:33:28.84 CS.seek 0ml @11:33:30.67 ATV.dial :ASV @11:33:31.68 ASV.dial :SPEdelivery @11:33:32.04 AFV.select :carrierSolution @11:33:32.15 delay 2 seconds @11:33:34.18 AS.reconfigure ASconfig @11:33:34.23 AS.seek 0.5ml @11:33:45.86
Flushing lower manifold's waste2b port @11:33:46.00 CSV.dial :flush @11:33:46.81 CS.seek 5ml @11:33:49.48 delay 1 second @11:33:50.50 AFV.select :holdingCoil @11:33:50.65 AS.reconfigure ASslow1 @11:33:50.70 AS.seek 0.17ml @11:33:59.23
delay 5 seconds @11:34:04.26 CSV.dial :air @11:34:04.82 CS.seek 7ml @11:34:10.48 CSV.dial CTV @11:34:10.52
CTV.dial CSV @11:34:10.98 CBV.dial :waste2b @11:34:11.81 CS.seek 0ml @11:34:28.86 Flushing upper manifold @11:34:28.99 CSV.dial :flush @11:34:30.44 CS.seek 5ml @11:34:42.86 delay 5 seconds @11:34:47.89 CSV.dial :air @11:34:48.45 CS.seek 7ml @11:34:54.08 CSV.dial CBV @11:34:54.11
CBV.dial CSV @11:34:55.38 CTV.dial :air @11:34:56.79 CS.seek 0ml @11:34:57.35 SPE.hold 2:46:40,95C @11:34:57.41 delay 3:00 @11:35:14.42
delay 7 seconds @11:35:21.45 CS.seek 3ml @11:35:29.55 CSV.dial :waste @11:35:30.04 CS.seek 0ml @11:35:38.33 CSV.dial :flush @11:35:38.82 CS.seek 3ml @11:35:46.92 delay 5 seconds @11:35:51.95 CSV.dial :air @11:35:52.51 CS.seek 5ml @11:35:58.33 CSV.dial :air @11:35:58.35 CS.seek 0ml @11:36:11.46 delay 7 seconds @11:36:18.50 CS.seek 3ml @11:36:26.74 CSV.dial :waste @11:36:27.56 CS.seek 0ml @11:36:35.80 Disinfecting external Intake with mfbkill @11:36:35.94 CSV.dial CSR @11:36:37.43 Pulling 8ml of CSR.mfbkill @11:36:37.44 CSR.select :mfbkill @11:36:37.56 CS.seek 8ml @11:36:57.68 delay 5 seconds @11:37:02.71 CSR.select :air @11:37:02.84 CS.seek 10ml @11:37:08.61 CSV.dial CBV @11:37:08.64
CBV.dial CSV @11:37:09.77 CTV.dial :intake @11:37:10.60 Intake.select :open @11:37:10.72 CS.seek 2ml @11:37:32.14 Intake.select :close @11:37:32.25 CTV.dial :air @11:37:33.08 CS.reconfigure CSconfig @11:37:33.14 CS.seek 0ml @11:37:39.27 Flushing lower manifold's waste1 port @11:37:39.28 CSV.dial CSR @11:37:40.48 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @11:37:40.61 CS.seek 4ml @11:37:51.77 delay 5 seconds @11:37:56.80 CSR.select :air @11:37:56.92 CS.reconfigure CSfast @11:37:56.97 CS.seek 10ml @11:37:57.46 AFV.select :carrierSolution @11:37:57.56 delay 2 seconds @11:37:59.59 AS.reconfigure ASconfig @11:37:59.64 AS.seek 1ml @11:38:12.36 CSV.dial CTV @11:38:12.40
CTV.dial CSV @11:38:13.93 CBV.dial :waste1 @11:38:14.45 CS.seek 0ml @11:38:39.36 Flushing lower manifold's waste2b port @11:38:39.37 CSV.dial CSR @11:38:39.92 Pulling 4ml of CSR.mfbkill @11:38:39.93 CSR.select :mfbkill @11:38:40.06 CS.reconfigure CSconfig @11:38:40.10 CS.seek 4ml @11:38:47.64 delay 1 second @11:38:48.66 AFV.select :holdingCoil @11:38:48.76 delay 1 second @11:38:49.79 ASV.dial :wasteD @11:38:50.80 AS.seek 0ml @11:38:51.27
delay 5 seconds @11:38:56.30 CSR.select :air @11:38:56.42 CS.reconfigure CSfast @11:38:56.46 CS.seek 10ml @11:39:11.58 CSV.dial CTV @11:39:11.64
CTV.dial CSV @11:39:12.12 CBV.dial :waste2b @11:39:12.97 CS.seek 0ml @11:39:37.58 Killing upper manifold @11:39:37.59 CSV.dial CSR @11:39:38.14 Pulling 4ml of CSR.mfbkill @11:39:38.15 CSR.select :mfbkill @11:39:38.28 CS.reconfigure CSconfig @11:39:38.33 CS.seek 4ml @11:39:48.58 Priming Eluate @11:39:48.59 AFV.select :carrierSolution @11:39:48.70 delay 2 seconds @11:39:49.62
delay 5 seconds @11:39:50.74 AS.seek 0.5ml @11:39:54.64
CSR.select :air @11:39:54.76 CS.reconfigure CSfast @11:39:54.80 CS.seek 10ml @11:40:09.77 CSV.dial CBV @11:40:09.81
CBV.dial CSV @11:40:11.07 CTV.dial :air @11:40:12.50 CS.seek 0ml @11:40:19.75 delay 1 second @11:40:20.77 AFV.select :holdingCoil @11:40:20.87 ATV.dial ASV @11:40:20.88 ASV.dial :SPErecovery @11:40:21.45 Swish #1 of 2 @11:40:21.53 AS.reconfigure ASslow0 @11:40:21.58 AS.seek 0.6ml @11:40:32.05 AS.seek 0.5ml @11:40:36.77
delay 7 seconds @11:40:42.45 Swish #2 of 2 @11:40:42.53 AS.seek 0.6ml @11:40:43.80
CS.seek 3ml @11:40:51.84 CSV.dial :waste @11:40:52.35 CS.seek 0ml @11:40:53.02 AS.seek 0.5ml @11:41:00.37
Flushing upper manifold @11:41:00.51 CSV.dial :flush @11:41:01.00 CS.seek 5ml @11:41:03.39 delay 3 seconds @11:41:06.48 AS.reconfigure ASslow1 @11:41:06.53 AS.seek 0.684ml @11:41:13.71
delay 5 seconds @11:41:18.74 CSV.dial :air @11:41:19.30 CS.seek 7ml @11:41:25.02 CSV.dial CBV @11:41:25.05
CBV.dial CSV @11:41:26.16 CTV.dial :air @11:41:26.18 CS.seek 0ml @11:41:43.46 delay 7 seconds @11:41:43.84 delay 3 seconds @11:41:46.87 Eluate primed @11:41:46.88 ATV.dial :waste2 @11:41:47.47 AS.reconfigure ASnorm @11:41:47.52 AS.seek 0ml @11:41:50.49
CS.seek 3ml @11:41:58.40 CSV.dial :waste @11:41:58.88 CS.seek 0ml @11:42:06.94 CSV.dial :flush @11:42:07.42 CS.seek 3ml @11:42:15.37 delay 5 seconds @11:42:20.40 CSV.dial :air @11:42:21.06 CS.seek 5ml @11:42:22.28 SPE.coast @11:42:22.36 ASV.dial :SPErecovery @11:42:22.38 Setting up PCR for OM -- writing /var/log/esp/OM20may2111h.pcr @11:42:22.40 Building PCR Slug from ASV with MM3 mix, PP5 primer @11:42:22.42 Priming with carrier @11:42:22.43 ATV.dial ARV @11:42:23.36 ARV.dial :carrier @11:42:23.71 AS.seek 0.5ml @11:42:26.93
CSV.dial :air @11:42:26.95 CS.seek 0ml @11:42:39.37 delay 7 seconds @11:42:46.41 CS.seek 3ml @11:42:49.33 AFV.select :carrierSolution @11:42:49.44 delay 2 seconds @11:42:51.47 AS.reconfigure ASconfig @11:42:51.53 AS.seek 1ml @11:42:54.24
CSV.dial :waste @11:42:55.00 CS.seek 0ml @11:43:02.93 Flushing lower manifold's waste1 port @11:43:03.07 CSV.dial :flush @11:43:03.57 CS.seek 5ml @11:43:16.68 delay 5 seconds @11:43:20.58 delay 1 second @11:43:21.60 AFV.select :holdingCoil @11:43:21.70
CSV.dial :air @11:43:21.76 delay 1 second @11:43:22.25
CS.seek 7ml @11:43:22.78 ATV.dial :waste @11:43:24.20 AS.reconfigure ASnorm @11:43:24.26 AS.seek :empty @11:43:27.96 CSV.dial CTV @11:43:28.00
CTV.dial CSV @11:43:29.46 CBV.dial :waste1 @11:43:29.96 CS.seek 0ml @11:43:46.96 Flushing lower manifold's waste2b port @11:43:47.10 CSV.dial :flush @11:43:47.90 CS.seek 5ml @11:44:00.31 delay 5 seconds @11:44:05.34 CSV.dial :air @11:44:05.89 CS.seek 7ml @11:44:11.53 CSV.dial CTV @11:44:11.56
CTV.dial CSV @11:44:12.03 CBV.dial :waste2b @11:44:12.85 CS.seek 0ml @11:44:15.00 AFV.select :carrierSolution @11:44:15.11 delay 2 seconds @11:44:17.14 AS.reconfigure ASconfig @11:44:17.20 AS.seek 0.5ml @11:44:29.75
Disinfecting external Intake with kill @11:44:29.89 CSV.dial CSR @11:44:30.42 Pulling 8ml of CSR.kill @11:44:30.43 CSR.select :kill @11:44:30.55 CS.seek 8ml @11:44:46.31 delay 1 second @11:44:47.33 AFV.select :holdingCoil @11:44:47.43 Building Slug @11:44:47.44 ATV.dial :ARV @11:44:48.98 delay 2 seconds @11:44:49.68
delay 5 seconds @11:44:51.01 ARV.dial :air1 @11:44:51.40 delay 2 seconds @11:44:53.49 AS.reconfigure ASslow2 @11:44:53.54 AS.seek 0.506ml @11:44:54.71
CSR.select :air @11:44:54.83 CS.seek 10ml @11:44:59.73 delay 2 seconds @11:45:00.53 CSV.dial CBV @11:45:00.56
CBV.dial CSV @11:45:01.68 CTV.dial :intake @11:45:01.75 ARV.dial :MM3 @11:45:02.47
Intake.select :open @11:45:02.59 CS.seek 2ml @11:45:02.77 delay 2 seconds @11:45:04.80 Adding MM3 @11:45:04.90 AS.seek 0.524ml @11:45:22.93
Intake.select :close @11:45:23.04 CTV.dial :air @11:45:23.20 delay 25 seconds @11:45:23.90
CS.reconfigure CSconfig @11:45:23.95 CS.seek 0ml @11:45:30.09 CSV.dial CSR @11:45:31.21 Pulling 5ml of CSR.flush @11:45:31.22 CSR.select :flush @11:45:31.35 CS.seek 5ml @11:45:45.31 delay 5 seconds @11:45:48.23 ARV.dial :PP5 @11:45:48.61 Adding PP5 @11:45:48.63 delay 2 seconds @11:45:50.34
CSR.select :air @11:45:50.47 CS.seek 10ml @11:45:50.71 AS.seek 0.53ml @11:45:56.92 delay 10 seconds @11:46:04.19
CSV.dial CBV @11:46:05.27 CS.seek 0ml @11:46:06.94 ARV.dial :air3 @11:46:07.31 adding ARV air @11:46:07.33 delay 2 seconds @11:46:09.42 AS.seek 0.536ml @11:46:15.69 delay 5 seconds @11:46:20.72 ARV.dial :carrier @11:46:21.92 delay 7 seconds @11:46:29.68 AS.seek 0.594ml @11:46:31.97
Garaging FlushPuck in Collection Clamp @11:46:32.05 Elbow.reconfigure TightElbow @11:46:32.10 Elbow.seek CC @11:46:32.33 Elbow.reconfigure LooseElbow @11:46:32.44 Forearm.seek CC @11:46:33.45 Hand.close @11:46:34.03 CC.seek :open @11:46:38.41 Forearm.seek :retract @11:46:39.41 Elbow.reconfigure TightElbow @11:46:39.46 Elbow.seek :garage @11:46:42.65 Elbow.reconfigure LooseElbow @11:46:42.75 Forearm.reconfigure TightForearm @11:46:42.80 Forearm.seek :garage @11:46:43.95 Hand.open @11:46:44.58 Forearm.reconfigure LooseForearm @11:46:44.63 Forearm.seek :retract @11:46:45.65 Starting initialPurge @11:46:45.66 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:46:45.74 Hand.open @11:46:46.08 Elbow.reconfigure TightElbow @11:46:46.13 Elbow.seek FlushPuck::Garage @11:46:46.33 Elbow.reconfigure LooseElbow @11:46:46.43 Forearm.seek :garage @11:46:47.42 Hand.close @11:46:48.14 Forearm.seek :retract @11:46:49.17 Elbow.reconfigure TightElbow @11:46:49.22 Elbow.seek CC @11:46:52.15 Elbow.reconfigure LooseElbow @11:46:52.26 Forearm.reconfigure TightForearm @11:46:52.31 Forearm.seek CC @11:46:53.63 CC.seek :closed @11:46:57.90 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.97 @11:46:57.97 Hand.open @11:46:58.71 Forearm.reconfigure LooseForearm @11:46:58.76 Forearm.seek :retract @11:46:59.78 Flushing the Collection Valve::Manifold @11:46:59.92 CSV.dial CSR @11:47:01.04 Pulling 5ml of CSR.flush @11:47:01.05 CSR.select :flush @11:47:01.17 CS.seek 5ml @11:47:15.03 delay 5 seconds @11:47:20.06 CSR.select :air @11:47:20.23 CS.reconfigure CSfast @11:47:20.29 CS.seek 7.501ml @11:47:27.09 CSV.dial :waste @11:47:28.30 delay 5 seconds @11:47:28.44
CS.reconfigure CSconfig @11:47:28.49 CS.seek 0ml @11:47:33.32 ATV.dial :ASV @11:47:33.70 delay 2 seconds @11:47:35.79 AS.seek 0.6ml @11:47:42.19 delay 4 seconds @11:47:46.22 ATV.dial ARV @11:47:46.74 delay 2 seconds @11:47:48.66
Purging external Intake @11:47:48.79 CSV.dial :flush @11:47:49.53 ARV.dial :carrier @11:47:49.55 delay 2 seconds @11:47:49.97
CS.reconfigure CSfast @11:47:50.02 CS.seek 10ml @11:47:51.64 AS.seek 0.65ml @11:48:13.78
delay 5 seconds @11:48:18.81 CSV.dial CBV @11:48:18.85
CBV.dial CSV @11:48:19.63 CTV.dial :intake @11:48:20.42 Intake.select :open @11:48:20.54 CS.seek 0ml @11:48:42.30 delay 4 seconds @11:48:45.59
Intake.select :close @11:48:45.70 CTV.dial :air @11:48:46.32 ATV.dial PCR @11:48:46.50
Flushing upper manifold @11:48:46.64 CSV.dial :flush @11:48:46.91 ASV.dial :waste1 @11:48:47.43
CS.seek 5ml @11:48:47.67 ARV.dial :air1 @11:48:48.06 Positioning Slug by 0.324ml @11:48:48.15 AS.seek 0.326ml @11:48:59.69
delay 5 seconds @11:49:04.72 CSV.dial :air @11:49:05.38 CS.seek 7ml @11:49:11.00 CSV.dial CBV @11:49:11.03
CBV.dial CSV @11:49:12.10 CTV.dial :air @11:49:12.12 CS.seek 0ml @11:49:28.91 delay 7 seconds @11:49:35.95 CS.seek 3ml @11:49:43.72 CSV.dial :waste @11:49:44.20 CS.seek 0ml @11:49:52.22 CSV.dial :flush @11:49:52.70 CS.seek 3ml @11:50:00.63 delay 5 seconds @11:50:05.66 CSV.dial :air @11:50:06.20 CS.seek 5.001ml @11:50:11.78 CSV.dial :air @11:50:11.80 CS.seek 0ml @11:50:24.16 delay 7 seconds @11:50:31.20 CS.seek 3ml @11:50:39.03 CSV.dial :waste @11:50:39.81 CS.seek 0ml @11:50:47.63 Garaging FlushPuck in Collection Clamp @11:50:47.71 Elbow.reconfigure TightElbow @11:50:47.76 Elbow.seek CC @11:50:47.99 Elbow.reconfigure LooseElbow @11:50:48.10 Forearm.seek CC @11:50:49.11 Hand.close @11:50:49.77 CC.seek :open @11:50:54.13 Forearm.seek :retract @11:50:55.16 Elbow.reconfigure TightElbow @11:50:55.21 Elbow.seek :garage @11:50:58.38 Elbow.reconfigure LooseElbow @11:50:58.48 Forearm.reconfigure TightForearm @11:50:58.53 Forearm.seek :garage @11:50:59.68 Hand.open @11:51:00.34 Forearm.reconfigure LooseForearm @11:51:00.39 Forearm.seek :retract @11:51:01.39 Completed [coreNEG] begun 10:00:25 @11:51:01.40 Preparing to sleep until Fri May 22 10:00:25 +0200 2020 @11:51:01.47 deferred until MFBPCR finish @11:51:01.49
Waiting for MFBPCR til 10:00CEST22-May-20 @11:51:01.50 Continuing at 10:00CEST22-May-20 @11:51:01.57 GoodNews.email "Then, in tube 3: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:150\n{1 => 22, 2 => 6, 3 => 16}\nCan@11:51:01, 10.7C, 46% humidity, 14.4psia, 13.456V, 0.481A, 0.481A avg, 6.47W",Subject: "Waiting until 10:00CEST22-May-20" @11:54:14.16 delay 5 seconds @11:54:19.19 ATV.dial ARV @11:54:19.93 ASV.dial :PCRlock @11:54:20.30 delay 5 seconds @11:54:25.33 Running OM Profile60C350 thermocycling @11:54:25.35 PCR.program Profile60C350 @11:54:25.46 Profile60C350 should complete in < 2:02:45 @11:54:25.47 PCR.run 2:38:26.25 @13:30:57.00 PCR.off @13:30:58.25 PCR for OM completed -- closed /var/log/esp/OM20may2111h.pcr @13:30:58.32 Cleaning PCR @13:30:58.33 AFV.select :carrierSolution @13:30:58.44 delay 2 seconds @13:31:00.47 AS.reconfigure ASconfig @13:31:00.52 AS.seek 0.5ml @13:31:10.82 delay 1 second @13:31:11.84 AFV.select :holdingCoil @13:31:11.94 ASV.dial :waste1 @13:31:12.32 ATV.dial :air3 @13:31:13.55 AS.reconfigure ASslow1 @13:31:13.60 AS.seek 0.51ml @13:31:15.94 delay 2 seconds @13:31:17.96 ATV.dial :bleach @13:31:18.56 AS.seek 0.81ml @13:32:19.15 delay 3 seconds @13:32:22.18 ATV.dial :air3 @13:32:22.71 delay 3 seconds @13:32:25.80 AS.seek 0.835ml @13:32:31.17 delay 3 seconds @13:32:34.20 ATV.dial :bleach @13:32:35.50 AS.seek 0.82ml @13:32:38.81 delay 3 seconds @13:32:41.84 ARV.dial :waste @13:32:42.43 delay 3 seconds @13:32:45.46 ATV.dial :ARV @13:32:46.31 AS.reconfigure ASconfig @13:32:46.36 AS.seek 0.47ml @13:33:06.79 AFV.select :carrierSolution @13:33:06.90 delay 2 seconds @13:33:08.94 AS.seek 1ml @13:33:39.70 delay 1 second @13:33:40.72 AFV.select :holdingCoil @13:33:40.82 delay 1 second @13:33:41.85 AS.reconfigure ASfast @13:33:41.90 AS.seek 0ml @13:34:09.24 AFV.select :carrierSolution @13:34:09.35 delay 2 seconds @13:34:11.38 AS.reconfigure ASconfig @13:34:11.43 AS.seek 1ml @13:35:09.20 delay 1 second @13:35:10.22 AFV.select :holdingCoil @13:35:10.32 delay 1 second @13:35:11.35 AS.reconfigure ASfast @13:35:11.40 AS.seek 0ml @13:35:38.76 AFV.select :carrierSolution @13:35:38.87 delay 2 seconds @13:35:40.90 AS.reconfigure ASconfig @13:35:40.95 AS.seek 1ml @13:36:38.75 delay 1 second @13:36:39.77 AFV.select :holdingCoil @13:36:39.87 delay 1 second @13:36:40.90 AS.reconfigure ASfast @13:36:40.95 AS.seek 0ml @13:37:08.29 ARV.dial between carrier and air1 @13:37:08.76 ATV.dial :air3 @13:37:10.00 AS.reconfigure ASslow1 @13:37:10.05 AS.seek 0.01ml @13:37:12.34 delay 2 seconds @13:37:14.36 ATV.dial :bleach @13:37:14.97 AS.seek 0.81ml @13:39:56.12 delay 3 seconds @13:39:59.15 ATV.dial :air3 @13:39:59.67 delay 3 seconds @13:40:02.76 AS.seek 0.835ml @13:40:08.07 delay 3 seconds @13:40:11.10 ATV.dial :bleach @13:40:11.71 AS.seek 0.82ml @13:40:14.95 delay 3 seconds @13:40:17.98 ATV.dial PCR @13:40:18.36 AS.reconfigure ASconfig @13:40:18.41 AS.seek :empty @13:41:05.87 PCR.program :bleachProfile @13:41:05.93 PCR program should complete in < 2:45 @13:41:05.94 PCR.run 10:00 @13:44:19.88 PCR.off @13:44:19.90 AFV.select :carrierSolution @13:44:20.01 delay 2 seconds @13:44:22.05 AS.seek 1ml @13:45:19.88 delay 1 second @13:45:20.90 AFV.select :holdingCoil @13:45:21.00 delay 1 second @13:45:22.03 AS.reconfigure ASfast @13:45:22.08 AS.seek 0ml @13:45:49.44 delay 3 seconds @13:45:52.47 AFV.select :carrierSolution @13:45:52.57 delay 2 seconds @13:45:54.60 AS.reconfigure ASconfig @13:45:54.66 AS.seek 1ml @13:46:52.46 delay 1 second @13:46:53.48 AFV.select :holdingCoil @13:46:53.58 delay 1 second @13:46:54.61 AS.reconfigure ASfast @13:46:54.66 AS.seek 0ml @13:47:22.00 delay 3 seconds @13:47:25.03 AFV.select :carrierSolution @13:47:25.13 delay 2 seconds @13:47:27.16 AS.reconfigure ASconfig @13:47:27.22 AS.seek 1ml @13:48:25.03 delay 1 second @13:48:26.05 AFV.select :holdingCoil @13:48:26.15 delay 1 second @13:48:27.18 AS.reconfigure ASfast @13:48:27.23 AS.seek 0ml @13:48:54.57 delay 3 seconds @13:48:57.60 AFV.select :carrierSolution @13:48:57.70 delay 2 seconds @13:48:59.73 AS.reconfigure ASconfig @13:48:59.79 AS.seek 1ml @13:49:57.57 delay 1 second @13:49:58.59 AFV.select :holdingCoil @13:49:58.69 delay 1 second @13:49:59.72 AS.reconfigure ASfast @13:49:59.77 AS.seek 0ml @13:50:27.11 delay 3 seconds @13:50:30.14 AFV.select :carrierSolution @13:50:30.24 delay 2 seconds @13:50:32.27 AS.reconfigure ASconfig @13:50:32.33 AS.seek 1ml @13:51:30.82 delay 1 second @13:51:31.84 AFV.select :holdingCoil @13:51:31.94 delay 1 second @13:51:32.97 AS.reconfigure ASnorm @13:51:33.02 AS.seek 0ml @13:52:23.76 Setting up PCR for ML -- writing /var/log/esp/ML20may2113h.pcr @13:52:23.78 Building PCR Slug from ASV with MM2 mix, PP4 primer @13:52:23.80 Priming with carrier @13:52:23.81 ATV.dial ARV @13:52:24.40 ARV.dial :carrier @13:52:24.75 AS.seek 0.5ml @13:52:50.33 AFV.select :carrierSolution @13:52:50.44 delay 2 seconds @13:52:52.47 AS.reconfigure ASconfig @13:52:52.52 AS.seek 1ml @13:53:21.58 delay 1 second @13:53:22.60 AFV.select :holdingCoil @13:53:22.70 delay 1 second @13:53:23.73 ATV.dial :waste @13:53:25.30 AS.reconfigure ASnorm @13:53:25.35 AS.seek :empty @13:54:16.12 AFV.select :carrierSolution @13:54:16.23 delay 2 seconds @13:54:18.26 AS.reconfigure ASconfig @13:54:18.31 AS.seek 0.5ml @13:54:47.43 delay 1 second @13:54:48.45 AFV.select :holdingCoil @13:54:48.55 Building Slug @13:54:48.56 ATV.dial :ARV @13:54:50.10 delay 2 seconds @13:54:52.13 ARV.dial :air1 @13:54:52.53 delay 2 seconds @13:54:54.61 AS.reconfigure ASslow2 @13:54:54.66 AS.seek 0.506ml @13:55:00.85 delay 2 seconds @13:55:02.88 ARV.dial :MM2 @13:55:04.27 delay 2 seconds @13:55:06.30 Adding MM2 @13:55:06.39 AS.seek 0.524ml @13:55:24.68 delay 25 seconds @13:55:49.71 ARV.dial :PP4 @13:55:50.09 Adding PP4 @13:55:50.11 delay 2 seconds @13:55:52.19 AS.seek 0.53ml @13:55:58.40 delay 10 seconds @13:56:08.43 ARV.dial :air3 @13:56:08.80 adding ARV air @13:56:08.82 delay 2 seconds @13:56:10.90 AS.seek 0.536ml @13:56:17.12 delay 5 seconds @13:56:22.15 ARV.dial :carrier @13:56:23.34 delay 7 seconds @13:56:30.43 AS.seek 0.594ml @13:57:28.91 delay 5 seconds @13:57:33.94 ATV.dial :ASV @13:57:34.34 delay 2 seconds @13:57:36.43 AS.seek 0.6ml @13:57:42.60 delay 4 seconds @13:57:46.63 ATV.dial ARV @13:57:47.15 delay 2 seconds @13:57:49.18 ARV.dial :carrier @13:57:49.19 delay 2 seconds @13:57:51.28 AS.seek 0.65ml @13:58:41.71 delay 4 seconds @13:58:45.74 ATV.dial PCR @13:58:46.32 ASV.dial :waste1 @13:58:46.33 ARV.dial :air1 @13:58:46.72 Positioning Slug by 0.324ml @13:58:46.81 AS.seek 0.326ml @14:04:12.81 delay 5 seconds @14:04:17.84 ATV.dial ARV @14:04:18.57 ASV.dial :PCRlock @14:04:18.95 delay 5 seconds @14:04:23.98 Running ML Profile60C350 thermocycling @14:04:24.00 PCR.program Profile60C350 @14:04:24.11 Profile60C350 should complete in < 2:02:45 @14:04:24.12 PCR.run 2:38:26.25 @15:40:33.64 PCR.off @15:40:34.92 PCR for ML completed -- closed /var/log/esp/ML20may2113h.pcr @15:40:34.99 Cleaning PCR @15:40:35.01 AFV.select :carrierSolution @15:40:35.11 delay 2 seconds @15:40:37.14 AS.reconfigure ASconfig @15:40:37.19 AS.seek 0.5ml @15:40:47.54 delay 1 second @15:40:48.56 AFV.select :holdingCoil @15:40:48.66 ASV.dial :waste1 @15:40:49.04 ATV.dial :air3 @15:40:50.26 AS.reconfigure ASslow1 @15:40:50.31 AS.seek 0.51ml @15:40:52.61 delay 2 seconds @15:40:54.63 ATV.dial :bleach @15:40:55.23 AS.seek 0.81ml @15:41:55.82 delay 3 seconds @15:41:58.85 ATV.dial :air3 @15:41:59.38 delay 3 seconds @15:42:02.47 AS.seek 0.835ml @15:42:07.75 delay 3 seconds @15:42:10.77 ATV.dial :bleach @15:42:11.37 AS.seek 0.82ml @15:42:14.63 delay 3 seconds @15:42:17.65 ARV.dial :waste @15:42:18.24 delay 3 seconds @15:42:21.27 ATV.dial :ARV @15:42:22.12 AS.reconfigure ASconfig @15:42:22.17 AS.seek 0.47ml @15:42:42.58 AFV.select :carrierSolution @15:42:42.69 delay 2 seconds @15:42:44.73 AS.seek 1ml @15:43:15.49 delay 1 second @15:43:16.51 AFV.select :holdingCoil @15:43:16.61 delay 1 second @15:43:17.64 AS.reconfigure ASfast @15:43:17.69 AS.seek 0ml @15:43:45.02 AFV.select :carrierSolution @15:43:45.13 delay 2 seconds @15:43:47.16 AS.reconfigure ASconfig @15:43:47.21 AS.seek 1ml @15:44:44.99 delay 1 second @15:44:46.01 AFV.select :holdingCoil @15:44:46.11 delay 1 second @15:44:47.14 AS.reconfigure ASfast @15:44:47.19 AS.seek 0ml @15:45:14.54 AFV.select :carrierSolution @15:45:14.65 delay 2 seconds @15:45:16.68 AS.reconfigure ASconfig @15:45:16.73 AS.seek 1ml @15:46:14.54 delay 1 second @15:46:15.56 AFV.select :holdingCoil @15:46:15.66 delay 1 second @15:46:16.69 AS.reconfigure ASfast @15:46:16.74 AS.seek 0ml @15:46:44.10 ARV.dial between carrier and air1 @15:46:44.57 ATV.dial :air3 @15:46:45.81 AS.reconfigure ASslow1 @15:46:45.86 AS.seek 0.01ml @15:46:48.16 delay 2 seconds @15:46:50.18 ATV.dial :bleach @15:46:50.78 AS.seek 0.81ml @15:49:32.60 delay 3 seconds @15:49:35.63 ATV.dial :air3 @15:49:36.17 delay 3 seconds @15:49:39.26 AS.seek 0.835ml @15:49:44.54 delay 3 seconds @15:49:47.57 ATV.dial :bleach @15:49:48.17 AS.seek 0.82ml @15:49:51.42 delay 3 seconds @15:49:54.45 ATV.dial PCR @15:49:54.84 AS.reconfigure ASconfig @15:49:54.89 AS.seek :empty @15:50:42.35 PCR.program :bleachProfile @15:50:42.41 PCR program should complete in < 2:45 @15:50:42.42 PCR.run 10:00 @15:53:56.36 PCR.off @15:53:56.39 AFV.select :carrierSolution @15:53:56.50 delay 2 seconds @15:53:58.54 AS.seek 1ml @15:54:56.34 delay 1 second @15:54:57.36 AFV.select :holdingCoil @15:54:57.46 delay 1 second @15:54:58.49 AS.reconfigure ASfast @15:54:58.54 AS.seek 0ml @15:55:25.87 delay 3 seconds @15:55:28.90 AFV.select :carrierSolution @15:55:29.00 delay 2 seconds @15:55:31.03 AS.reconfigure ASconfig @15:55:31.09 AS.seek 1ml @15:56:28.94 delay 1 second @15:56:29.96 AFV.select :holdingCoil @15:56:30.06 delay 1 second @15:56:31.09 AS.reconfigure ASfast @15:56:31.14 AS.seek 0ml @15:56:58.47 delay 3 seconds @15:57:01.50 AFV.select :carrierSolution @15:57:01.60 delay 2 seconds @15:57:03.63 AS.reconfigure ASconfig @15:57:03.68 AS.seek 1ml @15:58:01.45 delay 1 second @15:58:02.47 AFV.select :holdingCoil @15:58:02.57 delay 1 second @15:58:03.60 AS.reconfigure ASfast @15:58:03.65 AS.seek 0ml @15:58:30.98 delay 3 seconds @15:58:34.01 AFV.select :carrierSolution @15:58:34.11 delay 2 seconds @15:58:36.14 AS.reconfigure ASconfig @15:58:36.20 AS.seek 1ml @15:59:34.00 delay 1 second @15:59:35.02 AFV.select :holdingCoil @15:59:35.12 delay 1 second @15:59:36.15 AS.reconfigure ASfast @15:59:36.20 AS.seek 0ml @16:00:03.55 delay 3 seconds @16:00:06.58 AFV.select :carrierSolution @16:00:06.68 delay 2 seconds @16:00:08.71 AS.reconfigure ASconfig @16:00:08.76 AS.seek 1ml @16:01:06.56 delay 1 second @16:01:07.58 AFV.select :holdingCoil @16:01:07.68 delay 1 second @16:01:08.71 AS.reconfigure ASnorm @16:01:08.76 AS.seek 0ml @16:01:59.49 Setting up PCR for CG -- writing /var/log/esp/CG20may2116h.pcr @16:01:59.50 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:01:59.52 Priming with carrier @16:01:59.53 ATV.dial ARV @16:02:00.11 ARV.dial :carrier @16:02:00.45 AS.seek 0.5ml @16:02:26.08 AFV.select :carrierSolution @16:02:26.19 delay 2 seconds @16:02:28.22 AS.reconfigure ASconfig @16:02:28.27 AS.seek 1ml @16:02:57.30 delay 1 second @16:02:58.35 AFV.select :holdingCoil @16:02:58.45 delay 1 second @16:02:59.48 ATV.dial :waste @16:03:01.05 AS.reconfigure ASnorm @16:03:01.10 AS.seek :empty @16:03:51.86 AFV.select :carrierSolution @16:03:51.97 delay 2 seconds @16:03:54.00 AS.reconfigure ASconfig @16:03:54.05 AS.seek 0.5ml @16:04:23.13 delay 1 second @16:04:24.15 AFV.select :holdingCoil @16:04:24.25 Building Slug @16:04:24.26 ATV.dial :ARV @16:04:25.80 delay 2 seconds @16:04:27.83 ARV.dial :air1 @16:04:28.22 delay 2 seconds @16:04:30.31 AS.reconfigure ASslow2 @16:04:30.36 AS.seek 0.506ml @16:04:36.63 delay 2 seconds @16:04:38.65 ARV.dial :MM1 @16:04:39.22 delay 2 seconds @16:04:41.25 Adding MM1 @16:04:41.32 AS.seek 0.524ml @16:04:59.63 delay 25 seconds @16:05:25.33 ARV.dial :PP2 @16:05:25.74 Adding PP2 @16:05:25.76 delay 2 seconds @16:05:27.84 AS.seek 0.53ml @16:05:34.05 delay 10 seconds @16:05:44.08 ARV.dial :air2 @16:05:44.46 adding ARV air @16:05:44.48 delay 2 seconds @16:05:46.56 AS.seek 0.536ml @16:05:52.74 delay 5 seconds @16:05:57.77 ARV.dial :carrier @16:05:58.97 delay 7 seconds @16:06:06.07 AS.seek 0.594ml @16:07:04.58 delay 5 seconds @16:07:09.61 ATV.dial :ASV @16:07:10.01 delay 2 seconds @16:07:12.10 AS.seek 0.6ml @16:07:18.27 delay 4 seconds @16:07:22.30 ATV.dial ARV @16:07:22.83 delay 2 seconds @16:07:24.86 ARV.dial :carrier @16:07:24.87 delay 2 seconds @16:07:26.96 AS.seek 0.65ml @16:08:17.40 delay 4 seconds @16:08:21.43 ATV.dial PCR @16:08:22.02 ASV.dial :waste1 @16:08:22.03 ARV.dial :air1 @16:08:22.42 Positioning Slug by 0.324ml @16:08:22.51 AS.seek 0.326ml @16:13:48.71 delay 5 seconds @16:13:53.74 ATV.dial ARV @16:13:54.48 ASV.dial :PCRlock @16:13:54.87 delay 5 seconds @16:13:59.90 Running CG Profile60C300 thermocycling @16:13:59.91 PCR.program Profile60C300 @16:14:00.02 Profile60C300 should complete in < 1:59:25 @16:14:00.03 PCR.run 2:34:16.25 @17:49:25.04 PCR.off @17:49:25.71 PCR for CG completed -- closed /var/log/esp/CG20may2116h.pcr @17:49:25.77 Cleaning PCR @17:49:25.79 AFV.select :carrierSolution @17:49:25.90 delay 2 seconds @17:49:27.93 AS.reconfigure ASconfig @17:49:27.98 AS.seek 0.5ml @17:49:38.27 delay 1 second @17:49:39.29 AFV.select :holdingCoil @17:49:39.39 ASV.dial :waste1 @17:49:39.77 ATV.dial :air3 @17:49:40.98 AS.reconfigure ASslow1 @17:49:41.03 AS.seek 0.51ml @17:49:43.30 delay 2 seconds @17:49:45.32 ATV.dial :bleach @17:49:46.64 AS.seek 0.81ml @17:50:47.24 delay 3 seconds @17:50:50.27 ATV.dial :air3 @17:50:50.81 delay 3 seconds @17:50:53.90 AS.seek 0.835ml @17:50:59.19 delay 3 seconds @17:51:02.22 ATV.dial :bleach @17:51:02.83 AS.seek 0.82ml @17:51:06.12 delay 3 seconds @17:51:09.15 ARV.dial :waste @17:51:09.73 delay 3 seconds @17:51:12.76 ATV.dial :ARV @17:51:13.61 AS.reconfigure ASconfig @17:51:13.66 AS.seek 0.47ml @17:51:34.06 AFV.select :carrierSolution @17:51:34.17 delay 2 seconds @17:51:36.21 AS.seek 1ml @17:52:06.93 delay 1 second @17:52:07.95 AFV.select :holdingCoil @17:52:08.05 delay 1 second @17:52:09.08 AS.reconfigure ASfast @17:52:09.13 AS.seek 0ml @17:52:36.46 AFV.select :carrierSolution @17:52:36.57 delay 2 seconds @17:52:38.60 AS.reconfigure ASconfig @17:52:38.65 AS.seek 1ml @17:53:36.48 delay 1 second @17:53:37.50 AFV.select :holdingCoil @17:53:37.61 delay 1 second @17:53:38.64 AS.reconfigure ASfast @17:53:38.69 AS.seek 0ml @17:54:06.04 AFV.select :carrierSolution @17:54:06.15 delay 2 seconds @17:54:08.18 AS.reconfigure ASconfig @17:54:08.23 AS.seek 1ml @17:55:06.03 delay 1 second @17:55:07.05 AFV.select :holdingCoil @17:55:07.15 delay 1 second @17:55:08.18 AS.reconfigure ASfast @17:55:08.23 AS.seek 0ml @17:55:35.56 ARV.dial between carrier and air1 @17:55:36.04 ATV.dial :air3 @17:55:37.27 AS.reconfigure ASslow1 @17:55:37.32 AS.seek 0.01ml @17:55:39.59 delay 2 seconds @17:55:41.61 ATV.dial :bleach @17:55:42.22 AS.seek 0.81ml @17:58:23.37 delay 3 seconds @17:58:26.40 ATV.dial :air3 @17:58:26.94 delay 3 seconds @17:58:30.03 AS.seek 0.835ml @17:58:35.34 delay 3 seconds @17:58:38.37 ATV.dial :bleach @17:58:38.97 AS.seek 0.82ml @17:58:42.31 delay 3 seconds @17:58:45.33 ATV.dial PCR @17:58:45.73 AS.reconfigure ASconfig @17:58:45.78 AS.seek :empty @17:59:33.25 PCR.program :bleachProfile @17:59:33.30 PCR program should complete in < 2:45 @17:59:33.31 PCR.run 10:00 @18:02:47.25 PCR.off @18:02:47.28 AFV.select :carrierSolution @18:02:47.39 delay 2 seconds @18:02:49.43 AS.seek 1ml @18:03:47.25 delay 1 second @18:03:48.27 AFV.select :holdingCoil @18:03:48.37 delay 1 second @18:03:49.40 AS.reconfigure ASfast @18:03:49.45 AS.seek 0ml @18:04:16.81 delay 3 seconds @18:04:19.84 AFV.select :carrierSolution @18:04:19.94 delay 2 seconds @18:04:21.97 AS.reconfigure ASconfig @18:04:22.03 AS.seek 1ml @18:05:19.77 delay 1 second @18:05:20.79 AFV.select :holdingCoil @18:05:20.89 delay 1 second @18:05:21.92 AS.reconfigure ASfast @18:05:21.97 AS.seek 0ml @18:05:49.30 delay 3 seconds @18:05:52.33 AFV.select :carrierSolution @18:05:52.43 delay 2 seconds @18:05:54.47 AS.reconfigure ASconfig @18:05:54.62 AS.seek 1ml @18:06:52.49 delay 1 second @18:06:53.53 AFV.select :holdingCoil @18:06:53.69 delay 1 second @18:06:54.76 AS.reconfigure ASfast @18:06:54.89 AS.seek 0ml @18:07:22.34 delay 3 seconds @18:07:25.39 AFV.select :carrierSolution @18:07:25.57 delay 2 seconds @18:07:29.65 AS.reconfigure ASconfig @18:07:30.02 AS.seek 1ml @18:08:27.91 delay 1 second @18:08:28.94 AFV.select :holdingCoil @18:08:29.04 delay 1 second @18:08:30.07 AS.reconfigure ASfast @18:08:30.12 AS.seek 0ml @18:08:57.46 delay 3 seconds @18:09:00.49 AFV.select :carrierSolution @18:09:00.59 delay 2 seconds @18:09:02.62 AS.reconfigure ASconfig @18:09:02.68 AS.seek 1ml @18:10:00.52 delay 1 second @18:10:01.54 AFV.select :holdingCoil @18:10:01.64 delay 1 second @18:10:02.67 AS.reconfigure ASnorm @18:10:02.72 AS.seek 0ml @18:10:53.50 Setting up PCR for NM -- writing /var/log/esp/NM20may2118h.pcr @18:10:53.55 Building PCR Slug from ASV with MM1 mix, PP1 primer @18:10:53.56 Priming with carrier @18:10:53.57 ATV.dial ARV @18:10:54.16 ARV.dial :carrier @18:10:54.51 AS.seek 0.5ml @18:11:20.02 AFV.select :carrierSolution @18:11:20.13 delay 2 seconds @18:11:22.16 AS.reconfigure ASconfig @18:11:22.21 AS.seek 1ml @18:11:51.26 delay 1 second @18:11:52.28 AFV.select :holdingCoil @18:11:52.38 delay 1 second @18:11:53.41 ATV.dial :waste @18:11:54.97 AS.reconfigure ASnorm @18:11:55.03 AS.seek :empty @18:12:45.77 AFV.select :carrierSolution @18:12:45.88 delay 2 seconds @18:12:47.91 AS.reconfigure ASconfig @18:12:47.96 AS.seek 0.5ml @18:13:17.04 delay 1 second @18:13:18.06 AFV.select :holdingCoil @18:13:18.16 Building Slug @18:13:18.17 ATV.dial :ARV @18:13:19.71 delay 2 seconds @18:13:21.74 ARV.dial :air1 @18:13:22.13 delay 2 seconds @18:13:24.22 AS.reconfigure ASslow2 @18:13:24.27 AS.seek 0.506ml @18:13:30.60 delay 2 seconds @18:13:32.62 ARV.dial :MM1 @18:13:33.19 delay 2 seconds @18:13:35.22 Adding MM1 @18:13:35.29 AS.seek 0.524ml @18:13:53.79 delay 25 seconds @18:14:18.82 ARV.dial :PP1 @18:14:19.20 Adding PP1 @18:14:19.22 delay 2 seconds @18:14:21.30 AS.seek 0.53ml @18:14:27.52 delay 10 seconds @18:14:37.55 ARV.dial :air1 @18:14:37.93 adding ARV air @18:14:37.95 delay 2 seconds @18:14:40.03 AS.seek 0.536ml @18:14:46.20 delay 5 seconds @18:14:51.23 ARV.dial :carrier @18:14:51.60 delay 7 seconds @18:14:58.69 AS.seek 0.594ml @18:15:57.18 delay 5 seconds @18:16:02.21 ATV.dial :ASV @18:16:02.60 delay 2 seconds @18:16:04.69 AS.seek 0.6ml @18:16:10.91 delay 4 seconds @18:16:14.94 ATV.dial ARV @18:16:15.46 delay 2 seconds @18:16:17.49 ARV.dial :carrier @18:16:17.50 delay 2 seconds @18:16:19.59 AS.seek 0.65ml @18:17:10.20 delay 4 seconds @18:17:14.23 ATV.dial PCR @18:17:14.82 ASV.dial :waste1 @18:17:14.83 ARV.dial :air1 @18:17:15.22 Positioning Slug by 0.324ml @18:17:15.31 AS.seek 0.326ml @18:22:41.37 delay 5 seconds @18:22:46.41 ATV.dial ARV @18:22:47.14 ASV.dial :PCRlock @18:22:47.53 delay 5 seconds @18:22:52.56 Running NM Profile60C300 thermocycling @18:22:52.57 PCR.program Profile60C300 @18:22:52.71 Profile60C300 should complete in < 1:59:25 @18:22:52.72 PCR.run 2:34:16.25 @19:58:11.03 PCR.off @19:58:11.71 PCR for NM completed -- closed /var/log/esp/NM20may2118h.pcr @19:58:11.79 Cleaning PCR @19:58:11.80 AFV.select :carrierSolution @19:58:11.91 delay 2 seconds @19:58:13.94 AS.reconfigure ASconfig @19:58:13.99 AS.seek 0.5ml @19:58:24.29 delay 1 second @19:58:25.31 AFV.select :holdingCoil @19:58:25.41 ASV.dial :waste1 @19:58:25.79 ATV.dial :air3 @19:58:27.00 AS.reconfigure ASslow1 @19:58:27.05 AS.seek 0.51ml @19:58:29.35 delay 2 seconds @19:58:31.37 ATV.dial :bleach @19:58:31.97 AS.seek 0.81ml @19:59:32.56 delay 3 seconds @19:59:35.59 ATV.dial :air3 @19:59:36.12 delay 3 seconds @19:59:39.21 AS.seek 0.835ml @19:59:44.47 delay 3 seconds @19:59:47.50 ATV.dial :bleach @19:59:48.10 AS.seek 0.82ml @19:59:51.37 delay 3 seconds @19:59:54.40 ARV.dial :waste @19:59:54.98 delay 3 seconds @19:59:58.01 ATV.dial :ARV @19:59:58.85 AS.reconfigure ASconfig @19:59:58.91 AS.seek 0.47ml @20:00:19.33 AFV.select :carrierSolution @20:00:19.45 delay 2 seconds @20:00:21.49 AS.seek 1ml @20:00:52.23 delay 1 second @20:00:53.25 AFV.select :holdingCoil @20:00:53.35 delay 1 second @20:00:54.38 AS.reconfigure ASfast @20:00:54.43 AS.seek 0ml @20:01:21.73 AFV.select :carrierSolution @20:01:21.84 delay 2 seconds @20:01:23.87 AS.reconfigure ASconfig @20:01:23.92 AS.seek 1ml @20:02:21.67 delay 1 second @20:02:22.69 AFV.select :holdingCoil @20:02:22.79 delay 1 second @20:02:23.82 AS.reconfigure ASfast @20:02:23.87 AS.seek 0ml @20:02:51.22 AFV.select :carrierSolution @20:02:51.33 delay 2 seconds @20:02:53.36 AS.reconfigure ASconfig @20:02:53.41 AS.seek 1ml @20:03:51.17 delay 1 second @20:03:52.19 AFV.select :holdingCoil @20:03:52.29 delay 1 second @20:03:53.32 AS.reconfigure ASfast @20:03:53.37 AS.seek 0ml @20:04:20.70 ARV.dial between carrier and air1 @20:04:21.17 ATV.dial :air3 @20:04:22.40 AS.reconfigure ASslow1 @20:04:22.45 AS.seek 0.01ml @20:04:24.78 delay 2 seconds @20:04:26.80 ATV.dial :bleach @20:04:27.41 AS.seek 0.81ml @20:07:08.55 delay 3 seconds @20:07:11.57 ATV.dial :air3 @20:07:12.11 delay 3 seconds @20:07:15.20 AS.seek 0.835ml @20:07:21.16 delay 3 seconds @20:07:24.19 ATV.dial :bleach @20:07:24.79 AS.seek 0.82ml @20:07:28.10 delay 3 seconds @20:07:31.13 ATV.dial PCR @20:07:31.53 AS.reconfigure ASconfig @20:07:31.58 AS.seek :empty @20:08:19.03 PCR.program :bleachProfile @20:08:19.09 PCR program should complete in < 2:45 @20:08:19.10 PCR.run 10:00 @20:11:33.04 PCR.off @20:11:33.07 AFV.select :carrierSolution @20:11:33.18 delay 2 seconds @20:11:35.22 AS.seek 1ml @20:12:33.05 delay 1 second @20:12:34.07 AFV.select :holdingCoil @20:12:34.17 delay 1 second @20:12:35.20 AS.reconfigure ASfast @20:12:35.25 AS.seek 0ml @20:13:02.57 delay 3 seconds @20:13:05.60 AFV.select :carrierSolution @20:13:05.70 delay 2 seconds @20:13:07.73 AS.reconfigure ASconfig @20:13:07.79 AS.seek 1ml @20:14:05.55 delay 1 second @20:14:06.57 AFV.select :holdingCoil @20:14:06.67 delay 1 second @20:14:07.70 AS.reconfigure ASfast @20:14:07.75 AS.seek 0ml @20:14:35.08 delay 3 seconds @20:14:38.11 AFV.select :carrierSolution @20:14:38.21 delay 2 seconds @20:14:40.24 AS.reconfigure ASconfig @20:14:40.30 AS.seek 1ml @20:15:38.10 delay 1 second @20:15:39.12 AFV.select :holdingCoil @20:15:39.22 delay 1 second @20:15:40.25 AS.reconfigure ASfast @20:15:40.30 AS.seek 0ml @20:16:07.63 delay 3 seconds @20:16:10.66 AFV.select :carrierSolution @20:16:10.76 delay 2 seconds @20:16:12.79 AS.reconfigure ASconfig @20:16:12.85 AS.seek 1ml @20:17:10.63 delay 1 second @20:17:11.65 AFV.select :holdingCoil @20:17:11.75 delay 1 second @20:17:12.78 AS.reconfigure ASfast @20:17:12.83 AS.seek 0ml @20:17:40.17 delay 3 seconds @20:17:43.20 AFV.select :carrierSolution @20:17:43.30 delay 2 seconds @20:17:45.33 AS.reconfigure ASconfig @20:17:45.39 AS.seek 1ml @20:18:43.21 delay 1 second @20:18:44.23 AFV.select :holdingCoil @20:18:44.33 delay 1 second @20:18:45.36 AS.reconfigure ASnorm @20:18:45.41 AS.seek 0ml @20:19:36.12 Setting up PCR for IPc -- writing /var/log/esp/IPc20may2120h.pcr @20:19:36.13 Building PCR Slug from ASV with MM3 mix, PP6 primer @20:19:36.15 Priming with carrier @20:19:36.16 ATV.dial ARV @20:19:36.74 ARV.dial :carrier @20:19:37.08 AS.seek 0.5ml @20:20:02.67 AFV.select :carrierSolution @20:20:02.78 delay 2 seconds @20:20:04.81 AS.reconfigure ASconfig @20:20:04.86 AS.seek 1ml @20:20:33.93 delay 1 second @20:20:34.95 AFV.select :holdingCoil @20:20:35.05 delay 1 second @20:20:36.08 ATV.dial :waste @20:20:37.65 AS.reconfigure ASnorm @20:20:37.71 AS.seek :empty @20:21:28.46 AFV.select :carrierSolution @20:21:28.57 delay 2 seconds @20:21:30.60 AS.reconfigure ASconfig @20:21:30.65 AS.seek 0.5ml @20:21:59.73 delay 1 second @20:22:00.75 AFV.select :holdingCoil @20:22:00.85 Building Slug @20:22:00.86 ATV.dial :ARV @20:22:02.40 delay 2 seconds @20:22:04.43 ARV.dial :air1 @20:22:04.82 delay 2 seconds @20:22:06.91 AS.reconfigure ASslow2 @20:22:06.96 AS.seek 0.506ml @20:22:13.21 delay 2 seconds @20:22:15.23 ARV.dial :MM3 @20:22:16.23 delay 2 seconds @20:22:18.26 Adding MM3 @20:22:18.34 AS.seek 0.524ml @20:22:36.57 delay 25 seconds @20:23:01.60 ARV.dial :PP6 @20:23:01.98 Adding PP6 @20:23:01.99 delay 2 seconds @20:23:04.08 AS.seek 0.53ml @20:23:11.19 delay 10 seconds @20:23:21.23 ARV.dial :air1 @20:23:22.01 adding ARV air @20:23:22.03 delay 2 seconds @20:23:24.11 AS.seek 0.536ml @20:23:30.54 delay 5 seconds @20:23:35.57 ARV.dial :carrier @20:23:35.97 delay 7 seconds @20:23:43.06 AS.seek 0.594ml @20:24:41.61 delay 5 seconds @20:24:46.64 ATV.dial :ASV @20:24:47.03 delay 2 seconds @20:24:49.12 AS.seek 0.6ml @20:24:55.34 delay 4 seconds @20:24:59.37 ATV.dial ARV @20:25:00.00 delay 2 seconds @20:25:02.03 ARV.dial :carrier @20:25:02.04 delay 2 seconds @20:25:04.13 AS.seek 0.65ml @20:25:54.55 delay 4 seconds @20:25:58.58 ATV.dial PCR @20:25:59.17 ASV.dial :waste1 @20:25:59.18 ARV.dial :air1 @20:25:59.57 Positioning Slug by 0.324ml @20:25:59.66 AS.seek 0.326ml @20:31:25.66 delay 5 seconds @20:31:30.69 ATV.dial ARV @20:31:31.42 ASV.dial :PCRlock @20:31:31.80 delay 5 seconds @20:31:36.83 Running IPc ProfileIPc thermocycling @20:31:36.84 PCR.program ProfileIPc @20:31:36.95 ProfileIPc should complete in < 1:36:15 @20:31:36.96 PCR.run 2:05:18.75 @21:49:34.89 PCR.off @21:49:36.27 PCR for IPc completed -- closed /var/log/esp/IPc20may2120h.pcr @21:49:36.34 Cleaning PCR @21:49:36.36 AFV.select :carrierSolution @21:49:36.46 delay 2 seconds @21:49:38.54 AS.reconfigure ASconfig @21:49:38.58 AS.seek 0.5ml @21:49:48.88 delay 1 second @21:49:49.90 AFV.select :holdingCoil @21:49:50.00 ASV.dial :waste1 @21:49:50.38 ATV.dial :air3 @21:49:51.61 AS.reconfigure ASslow1 @21:49:51.66 AS.seek 0.51ml @21:49:53.97 delay 2 seconds @21:49:55.99 ATV.dial :bleach @21:49:56.59 AS.seek 0.81ml @21:50:57.16 delay 3 seconds @21:51:00.19 ATV.dial :air3 @21:51:00.72 delay 3 seconds @21:51:03.81 AS.seek 0.835ml @21:51:09.11 delay 3 seconds @21:51:12.13 ATV.dial :bleach @21:51:12.73 AS.seek 0.82ml @21:51:16.04 delay 3 seconds @21:51:19.07 ARV.dial :waste @21:51:19.65 delay 3 seconds @21:51:22.68 ATV.dial :ARV @21:51:23.54 AS.reconfigure ASconfig @21:51:23.59 AS.seek 0.47ml @21:51:44.01 AFV.select :carrierSolution @21:51:44.12 delay 2 seconds @21:51:46.16 AS.seek 1ml @21:52:16.96 delay 1 second @21:52:17.98 AFV.select :holdingCoil @21:52:18.08 delay 1 second @21:52:19.11 AS.reconfigure ASfast @21:52:19.16 AS.seek 0ml @21:52:46.52 AFV.select :carrierSolution @21:52:46.63 delay 2 seconds @21:52:48.66 AS.reconfigure ASconfig @21:52:48.71 AS.seek 1ml @21:53:46.49 delay 1 second @21:53:47.51 AFV.select :holdingCoil @21:53:47.61 delay 1 second @21:53:48.64 AS.reconfigure ASfast @21:53:48.69 AS.seek 0ml @21:54:16.05 AFV.select :carrierSolution @21:54:16.16 delay 2 seconds @21:54:18.19 AS.reconfigure ASconfig @21:54:18.24 AS.seek 1ml @21:55:16.04 delay 1 second @21:55:17.06 AFV.select :holdingCoil @21:55:17.16 delay 1 second @21:55:18.19 AS.reconfigure ASfast @21:55:18.24 AS.seek 0ml @21:55:45.60 ARV.dial between carrier and air1 @21:55:46.09 ATV.dial :air3 @21:55:47.33 AS.reconfigure ASslow1 @21:55:47.38 AS.seek 0.01ml @21:55:49.70 delay 2 seconds @21:55:51.72 ATV.dial :bleach @21:55:52.33 AS.seek 0.81ml @21:58:33.46 delay 3 seconds @21:58:36.49 ATV.dial :air3 @21:58:37.03 delay 3 seconds @21:58:40.12 AS.seek 0.835ml @21:58:45.43 delay 3 seconds @21:58:48.45 ATV.dial :bleach @21:58:49.05 AS.seek 0.82ml @21:58:52.35 delay 3 seconds @21:58:55.37 ATV.dial PCR @21:58:55.76 AS.reconfigure ASconfig @21:58:55.81 AS.seek :empty @21:59:43.26 PCR.program :bleachProfile @21:59:43.31 PCR program should complete in < 2:45 @21:59:43.32 PCR.run 10:00 @22:02:57.26 PCR.off @22:02:57.29 AFV.select :carrierSolution @22:02:57.40 delay 2 seconds @22:02:59.44 AS.seek 1ml @22:03:57.33 delay 1 second @22:03:58.35 AFV.select :holdingCoil @22:03:58.45 delay 1 second @22:03:59.48 AS.reconfigure ASfast @22:03:59.53 AS.seek 0ml @22:04:26.86 delay 3 seconds @22:04:29.89 AFV.select :carrierSolution @22:04:29.99 delay 2 seconds @22:04:32.02 AS.reconfigure ASconfig @22:04:32.08 AS.seek 1ml @22:05:30.56 delay 1 second @22:05:31.58 AFV.select :holdingCoil @22:05:31.68 delay 1 second @22:05:32.71 AS.reconfigure ASfast @22:05:32.76 AS.seek 0ml @22:06:00.11 delay 3 seconds @22:06:03.14 AFV.select :carrierSolution @22:06:03.24 delay 2 seconds @22:06:05.27 AS.reconfigure ASconfig @22:06:05.33 AS.seek 1ml @22:07:03.12 delay 1 second @22:07:04.14 AFV.select :holdingCoil @22:07:04.24 delay 1 second @22:07:05.27 AS.reconfigure ASfast @22:07:05.32 AS.seek 0ml @22:07:32.66 delay 3 seconds @22:07:35.69 AFV.select :carrierSolution @22:07:35.79 delay 2 seconds @22:07:37.82 AS.reconfigure ASconfig @22:07:37.88 AS.seek 1ml @22:08:35.67 delay 1 second @22:08:36.69 AFV.select :holdingCoil @22:08:36.79 delay 1 second @22:08:37.82 AS.reconfigure ASfast @22:08:37.87 AS.seek 0ml @22:09:05.22 delay 3 seconds @22:09:08.25 AFV.select :carrierSolution @22:09:08.35 delay 2 seconds @22:09:10.38 AS.reconfigure ASconfig @22:09:10.44 AS.seek 1ml @22:10:08.25 delay 1 second @22:10:09.27 AFV.select :holdingCoil @22:10:09.37 delay 1 second @22:10:10.40 AS.reconfigure ASnorm @22:10:10.45 AS.seek 0ml @22:11:01.18 Setting up PCR for IPc2 -- writing /var/log/esp/IPc220may2122h.pcr @22:11:01.22 Building PCR Slug from ASV with MM2 mix, PP3 primer @22:11:01.23 Priming with carrier @22:11:01.24 ATV.dial ARV @22:11:01.83 ARV.dial :carrier @22:11:02.18 AS.seek 0.5ml @22:11:27.74 AFV.select :carrierSolution @22:11:27.85 delay 2 seconds @22:11:29.88 AS.reconfigure ASconfig @22:11:29.93 AS.seek 1ml @22:11:59.00 delay 1 second @22:12:00.02 AFV.select :holdingCoil @22:12:00.12 delay 1 second @22:12:01.15 ATV.dial :waste @22:12:02.71 AS.reconfigure ASnorm @22:12:02.76 AS.seek :empty @22:12:53.50 AFV.select :carrierSolution @22:12:53.61 delay 2 seconds @22:12:55.64 AS.reconfigure ASconfig @22:12:55.69 AS.seek 0.5ml @22:13:24.77 delay 1 second @22:13:25.79 AFV.select :holdingCoil @22:13:25.89 Building Slug @22:13:25.90 ATV.dial :ARV @22:13:27.44 delay 2 seconds @22:13:29.47 ARV.dial :air1 @22:13:29.86 delay 2 seconds @22:13:31.95 AS.reconfigure ASslow2 @22:13:32.00 AS.seek 0.506ml @22:13:38.24 delay 2 seconds @22:13:40.26 ARV.dial :MM2 @22:13:41.66 delay 2 seconds @22:13:43.69 Adding MM2 @22:13:43.76 AS.seek 0.524ml @22:14:02.05 delay 25 seconds @22:14:27.08 ARV.dial :PP3 @22:14:27.46 Adding PP3 @22:14:27.47 delay 2 seconds @22:14:29.56 AS.seek 0.53ml @22:14:35.82 delay 10 seconds @22:14:45.85 ARV.dial :air2 @22:14:46.24 adding ARV air @22:14:46.25 delay 2 seconds @22:14:48.34 AS.seek 0.536ml @22:14:54.72 delay 5 seconds @22:14:59.75 ARV.dial :carrier @22:15:00.96 delay 7 seconds @22:15:08.05 AS.seek 0.594ml @22:16:06.57 delay 5 seconds @22:16:11.60 ATV.dial :ASV @22:16:11.99 delay 2 seconds @22:16:14.08 AS.seek 0.6ml @22:16:20.27 delay 4 seconds @22:16:24.30 ATV.dial ARV @22:16:24.82 delay 2 seconds @22:16:26.85 ARV.dial :carrier @22:16:26.86 delay 2 seconds @22:16:28.95 AS.seek 0.65ml @22:17:19.38 delay 4 seconds @22:17:23.41 ATV.dial PCR @22:17:23.99 ASV.dial :waste1 @22:17:24.00 ARV.dial :air1 @22:17:24.39 Positioning Slug by 0.324ml @22:17:24.48 AS.seek 0.326ml @22:22:50.48 delay 5 seconds @22:22:55.51 ATV.dial ARV @22:22:56.24 ASV.dial :PCRlock @22:22:57.31 delay 5 seconds @22:23:02.34 Running IPc2 ProfileIPc thermocycling @22:23:02.35 PCR.program ProfileIPc @22:23:02.46 ProfileIPc should complete in < 1:36:15 @22:23:02.47 PCR.run 2:05:18.75 @23:41:02.70 PCR.off @23:41:03.25 PCR for IPc2 completed -- closed /var/log/esp/IPc220may2122h.pcr @23:41:03.27 Full MFB Decontamination @23:41:03.28 Decon extraction column and decon AIV @23:41:03.30 SPE.hold 2:46:40,55C @23:41:03.34 ASV.dial :SPEdelivery @23:41:03.97 delay 20 seconds @23:41:24.00 AFV.select :carrierSolution @23:41:24.10 delay 2 seconds @23:41:26.13 AS.reconfigure ASconfig @23:41:26.18 AS.seek 0.5ml @23:41:36.49 delay 1 second @23:41:37.51 AFV.select :holdingCoil @23:41:37.61 ATV.dial :air1 @23:41:39.44 AS.reconfigure ASslow1 @23:41:39.49 AS.seek 0.52ml @23:41:43.87 ATV.dial :ASV @23:41:45.57 ASV.dial :SPErecovery @23:41:46.25 AS.seek 0.62ml @23:42:06.63 ATV.dial :waste @23:42:08.54 AS.reconfigure ASnorm @23:42:08.59 AS.seek :empty @23:42:40.21 AFV.select :holdingCoil @23:42:40.22 ATV.dial :waste @23:42:40.24 AS.reconfigure ASconfig @23:42:40.29 AS.seek 0ml @23:42:40.56 AFV.select :carrierSolution @23:42:40.69 delay 2 seconds @23:42:42.73 AS.seek 0.5ml @23:43:11.76 delay 1 second @23:43:12.78 AFV.select :holdingCoil @23:43:12.88 ATV.dial :air3 @23:43:13.58 AS.reconfigure ASslow1 @23:43:13.63 AS.seek 0.52ml @23:43:18.63 ATV.dial :air3 @23:43:18.72 AS.seek 0.53ml @23:43:20.98 delay 2 seconds @23:43:23.00 ATV.dial :bleach @23:43:23.48 AS.seek 0.71ml @23:43:59.93 delay 3 seconds @23:44:02.96 ATV.dial :air3 @23:44:03.51 delay 3 seconds @23:44:06.60 AS.seek 0.735ml @23:44:11.89 delay 3 seconds @23:44:14.92 ATV.dial :bleach @23:44:15.55 AS.seek 0.72ml @23:44:18.87 delay 3 seconds @23:44:21.90 ATV.dial ASV @23:44:22.96 ASV.dial :SPErecovery @23:44:23.05 AS.seek 0.365ml @23:45:34.70 ATV.dial :waste @23:45:36.35 AS.reconfigure ASnorm @23:45:36.40 AS.seek :empty @23:45:55.18 ATV.dial :air3 @23:45:55.86 AS.reconfigure ASslow1 @23:45:55.91 AS.seek 0.009ml @23:45:58.27 delay 2 seconds @23:46:00.29 ATV.dial :bleach @23:46:00.77 AS.seek 0.579ml @23:47:55.65 delay 3 seconds @23:47:58.68 ATV.dial :air3 @23:47:59.21 delay 3 seconds @23:48:02.30 AS.seek 0.604ml @23:48:07.57 delay 3 seconds @23:48:10.60 ATV.dial :bleach @23:48:11.21 AS.seek 0.589ml @23:48:14.51 delay 3 seconds @23:48:17.54 ATV.dial :ASV @23:48:18.53 ASV.dial :wasteR @23:48:18.93 AS.seek 0ml @23:50:17.70 delay 1:00 @23:51:17.73 ATV.dial ASV @23:51:17.74 AFV.select :carrierSolution @23:51:17.84 delay 2 seconds @23:51:19.87 AS.reconfigure ASconfig @23:51:19.92 AS.seek 1ml @23:52:17.68 delay 1 second @23:52:18.70 AFV.select :holdingCoil @23:52:18.80 delay 1 second @23:52:19.83 ASV.dial :wasteR @23:52:19.85 AS.reconfigure ASslow1 @23:52:19.89 AS.seek 0ml @23:55:41.31 delay 10 seconds @23:55:51.34 ATV.dial :air3 @23:55:52.77 AS.seek 0.01ml @23:55:55.06 delay 2 seconds @23:55:57.08 ATV.dial :bleach @23:55:57.69 AS.seek 0.58ml @23:57:52.59 delay 3 seconds @23:57:55.62 ATV.dial :air3 @23:57:56.16 delay 3 seconds @23:57:59.25 AS.seek 0.605ml @23:58:04.59 delay 3 seconds @23:58:07.62 ATV.dial :bleach @23:58:08.23 AS.seek 0.59ml @23:58:11.50 delay 3 seconds @23:58:14.53 ATV.dial ASV @23:58:15.52 ASV.dial :SPEdelivery @23:58:15.88 AS.seek 0ml @00:00:14.81CEST22-May-20 delay 1:00 @00:01:14.84 AFV.select :carrierSolution @00:01:14.94 delay 2 seconds @00:01:16.97 AS.reconfigure ASconfig @00:01:17.02 AS.seek 1ml @00:02:14.83 delay 1 second @00:02:15.85 AFV.select :holdingCoil @00:02:15.95 delay 1 second @00:02:16.98 ATV.dial ASV @00:02:16.99 ASV.dial :SPEdelivery @00:02:17.01 AS.reconfigure ASslow1 @00:02:17.05 AS.seek 0ml @00:05:38.41 delay 10 seconds @00:05:48.44 ATV.dial :air2 @00:05:49.23 AS.reconfigure ASslow0 @00:05:49.27 AS.seek 0.8ml @00:07:10.05 delay 3 seconds @00:07:13.08 ATV.dial :ASV @00:07:14.02 ASV.dial :SPEdelivery @00:07:14.04 AS.reconfigure ASslow1 @00:07:14.09 AS.seek 0.03ml @00:09:49.22 delay 20 seconds @00:10:09.25 ATV.dial :waste,via: :air3 @00:10:11.55 AS.reconfigure ASconfig @00:10:11.60 AS.seek :empty @00:10:13.67 SPE.coast @00:10:13.77 Removing Bleach from Mixing Coil @00:10:13.78 AFV.select :carrierSolution @00:10:13.88 delay 2 seconds @00:10:15.92 AS.seek 0.5ml @00:10:45.02 delay 1 second @00:10:46.04 AFV.select :holdingCoil @00:10:46.14 ATV.dial :ASV @00:10:47.87 ASV.dial :SPErecovery @00:10:48.51 AS.reconfigure ASslow1 @00:10:48.56 AS.seek 0.73ml @00:11:35.08 ASV.dial :wasteD @00:11:35.63 AS.reconfigure ASnorm @00:11:35.68 AS.seek 0ml @00:12:12.79 AFV.select :carrierSolution @00:12:12.90 delay 2 seconds @00:12:14.93 AS.reconfigure ASconfig @00:12:14.98 AS.seek 0.5ml @00:12:44.08 delay 1 second @00:12:45.10 AFV.select :holdingCoil @00:12:45.20 ATV.dial ASV @00:12:45.21 ASV.dial :SPErecovery @00:12:45.86 AS.reconfigure ASslow0 @00:12:45.91 AS.seek 0.3ml @00:13:06.37 ATV.dial :air2 @00:13:07.23 AS.reconfigure ASslow1 @00:13:07.28 AS.seek 0.32ml @00:13:11.58 ATV.dial ASV @00:13:12.58 AS.reconfigure ASslow0 @00:13:12.63 AS.seek 0.57ml @00:13:38.07 ASV.dial :wasteD @00:13:38.65 AS.reconfigure ASnorm @00:13:38.70 AS.seek 0ml @00:14:07.77 AFV.select :carrierSolution @00:14:07.88 delay 2 seconds @00:14:09.91 AS.reconfigure ASconfig @00:14:09.96 AS.seek 0.5ml @00:14:39.02 delay 1 second @00:14:40.04 AFV.select :holdingCoil @00:14:40.14 ATV.dial ASV @00:14:40.15 ASV.dial :SPErecovery @00:14:40.80 AS.reconfigure ASslow0 @00:14:40.85 AS.seek 0.3ml @00:15:01.30 ATV.dial :air2 @00:15:02.30 AS.reconfigure ASslow1 @00:15:02.35 AS.seek 0.32ml @00:15:06.68 ATV.dial ASV @00:15:07.69 AS.reconfigure ASslow0 @00:15:07.74 AS.seek 0.57ml @00:15:33.20 ASV.dial :wasteD @00:15:33.77 AS.reconfigure ASnorm @00:15:33.82 AS.seek 0ml @00:16:02.88 ATV.dial :air2 @00:16:03.81 AS.reconfigure ASslow0 @00:16:03.86 AS.seek 0.3ml @00:16:34.37 ATV.dial :ASV @00:16:35.31 ASV.dial :wasteD @00:16:35.33 AS.reconfigure ASconfig @00:16:35.38 AS.seek 0.05ml @00:16:50.06 ATV.dial :waste @00:16:51.83 AS.seek 0ml @00:16:55.00 ASV.dial :SPEdelivery @00:16:55.95 deconLysateINT @00:16:55.96 ATV.dial :air3 @00:16:56.63 AS.reconfigure ASslow1 @00:16:56.68 AS.seek 0.01ml @00:16:59.01 delay 2 seconds @00:17:01.03 ATV.dial :bleach @00:17:01.48 AS.seek 0.96ml @00:20:12.78 delay 3 seconds @00:20:15.81 ATV.dial :air3 @00:20:16.35 delay 3 seconds @00:20:19.44 AS.seek 0.985ml @00:20:24.71 delay 3 seconds @00:20:27.74 ATV.dial :bleach @00:20:28.35 AS.seek 0.97ml @00:20:31.67 delay 3 seconds @00:20:34.70 ATV.dial AIV @00:20:35.89 AIV.dial :coilToMFB @00:20:35.91 AWV.select :waste @00:20:35.92 AFV.select :carrierSolution @00:20:36.03 delay 2 seconds @00:20:38.06 AS.reconfigure ASconfig @00:20:38.81 AS.seek 1ml @00:20:40.89 delay 1 second @00:20:41.91 AFV.select :holdingCoil @00:20:42.01 delay 1 second @00:20:43.04 AS.reconfigure ASslow0 @00:20:43.10 AS.seek :empty @00:22:24.04 AFV.select :carrierSolution @00:22:24.15 delay 2 seconds @00:22:26.18 AS.reconfigure ASconfig @00:22:26.23 AS.seek 1ml @00:23:24.07 delay 1 second @00:23:25.09 AFV.select :holdingCoil @00:23:25.19 delay 1 second @00:23:26.22 AS.reconfigure ASslow0 @00:23:26.27 AS.seek :empty @00:25:07.25 AFV.select :carrierSolution @00:25:07.36 delay 2 seconds @00:25:09.39 AS.reconfigure ASconfig @00:25:09.44 AS.seek 1ml @00:26:07.25 delay 1 second @00:26:08.27 AFV.select :holdingCoil @00:26:08.37 delay 1 second @00:26:09.40 AS.reconfigure ASslow0 @00:26:09.45 AS.seek :empty @00:27:50.44 ATV.dial :air2 @00:27:51.10 AS.reconfigure ASslow1 @00:27:51.15 AS.seek 0.009ml @00:27:53.45 AS.reconfigure ASslow0 @00:27:53.50 AS.seek 1ml @00:29:33.44 ATV.dial AIV @00:29:34.20 AS.seek 0.02ml @00:31:13.06 ATV.dial :waste @00:31:14.62 AS.seek 0ml @00:31:16.95 AWV.select :air @00:31:17.11 Cleaning PCR @00:31:17.12 AFV.select :carrierSolution @00:31:17.23 delay 2 seconds @00:31:19.26 AS.reconfigure ASconfig @00:31:19.31 AS.seek 0.5ml @00:31:48.36 delay 1 second @00:31:49.38 AFV.select :holdingCoil @00:31:49.48 ASV.dial :waste1 @00:31:49.84 ATV.dial :air3 @00:31:50.49 AS.reconfigure ASslow1 @00:31:50.54 AS.seek 0.51ml @00:31:52.83 delay 2 seconds @00:31:54.85 ATV.dial :bleach @00:31:55.30 AS.seek 0.81ml @00:32:55.92 delay 3 seconds @00:32:58.95 ATV.dial :air3 @00:32:59.49 delay 3 seconds @00:33:02.58 AS.seek 0.835ml @00:33:07.95 delay 3 seconds @00:33:10.98 ATV.dial :bleach @00:33:11.59 AS.seek 0.82ml @00:33:14.88 delay 3 seconds @00:33:17.91 ARV.dial :waste @00:33:18.50 delay 3 seconds @00:33:21.53 ATV.dial :ARV @00:33:22.38 AS.reconfigure ASconfig @00:33:22.43 AS.seek 0.47ml @00:33:42.84 AFV.select :carrierSolution @00:33:42.95 delay 2 seconds @00:33:44.99 AS.seek 1ml @00:34:15.81 delay 1 second @00:34:16.83 AFV.select :holdingCoil @00:34:16.93 delay 1 second @00:34:17.96 AS.reconfigure ASfast @00:34:18.01 AS.seek 0ml @00:34:45.36 AFV.select :carrierSolution @00:34:45.47 delay 2 seconds @00:34:47.50 AS.reconfigure ASconfig @00:34:47.55 AS.seek 1ml @00:35:45.39 delay 1 second @00:35:46.41 AFV.select :holdingCoil @00:35:46.51 delay 1 second @00:35:47.54 AS.reconfigure ASfast @00:35:47.59 AS.seek 0ml @00:36:14.94 AFV.select :carrierSolution @00:36:15.05 delay 2 seconds @00:36:17.08 AS.reconfigure ASconfig @00:36:17.13 AS.seek 1ml @00:37:14.96 delay 1 second @00:37:15.98 AFV.select :holdingCoil @00:37:16.08 delay 1 second @00:37:17.11 AS.reconfigure ASfast @00:37:17.16 AS.seek 0ml @00:37:44.50 ARV.dial between carrier and air1 @00:37:44.97 ATV.dial :air3 @00:37:46.21 AS.reconfigure ASslow1 @00:37:46.26 AS.seek 0.01ml @00:37:48.61 delay 2 seconds @00:37:50.63 ATV.dial :bleach @00:37:51.23 AS.seek 0.81ml @00:40:32.38 delay 3 seconds @00:40:35.41 ATV.dial :air3 @00:40:35.94 delay 3 seconds @00:40:39.03 AS.seek 0.835ml @00:40:44.31 delay 3 seconds @00:40:48.02 ATV.dial :bleach @00:40:48.62 AS.seek 0.82ml @00:40:51.93 delay 3 seconds @00:40:54.96 ATV.dial PCR @00:40:55.36 AS.reconfigure ASconfig @00:40:55.41 AS.seek :empty @00:41:42.88 PCR.program :bleachProfile @00:41:42.94 PCR program should complete in < 2:45 @00:41:42.95 PCR.run 10:00 @00:45:09.01 PCR.off @00:45:09.03 AFV.select :carrierSolution @00:45:09.14 delay 2 seconds @00:45:11.18 AS.seek 1ml @00:46:08.96 delay 1 second @00:46:09.98 AFV.select :holdingCoil @00:46:10.08 delay 1 second @00:46:11.11 AS.reconfigure ASfast @00:46:11.16 AS.seek 0ml @00:46:38.54 delay 3 seconds @00:46:41.57 AFV.select :carrierSolution @00:46:41.67 delay 2 seconds @00:46:43.70 AS.reconfigure ASconfig @00:46:43.75 AS.seek 1ml @00:47:41.57 delay 1 second @00:47:42.59 AFV.select :holdingCoil @00:47:42.69 delay 1 second @00:47:43.72 AS.reconfigure ASfast @00:47:43.77 AS.seek 0ml @00:48:11.13 delay 3 seconds @00:48:14.16 AFV.select :carrierSolution @00:48:14.26 delay 2 seconds @00:48:16.29 AS.reconfigure ASconfig @00:48:16.35 AS.seek 1ml @00:49:14.12 delay 1 second @00:49:15.14 AFV.select :holdingCoil @00:49:15.24 delay 1 second @00:49:16.27 AS.reconfigure ASfast @00:49:16.32 AS.seek 0ml @00:49:43.67 delay 3 seconds @00:49:46.70 AFV.select :carrierSolution @00:49:46.80 delay 2 seconds @00:49:48.83 AS.reconfigure ASconfig @00:49:48.88 AS.seek 1ml @00:50:46.73 delay 1 second @00:50:47.75 AFV.select :holdingCoil @00:50:47.85 delay 1 second @00:50:48.88 AS.reconfigure ASfast @00:50:48.93 AS.seek 0ml @00:51:16.29 delay 3 seconds @00:51:19.32 AFV.select :carrierSolution @00:51:19.42 delay 2 seconds @00:51:21.45 AS.reconfigure ASconfig @00:51:21.50 AS.seek 1ml @00:52:19.34 delay 1 second @00:52:20.36 AFV.select :holdingCoil @00:52:20.46 delay 1 second @00:52:21.49 AS.reconfigure ASnorm @00:52:21.54 AS.seek 0ml @00:53:12.27 AFV.select :carrierSolution @00:53:12.38 delay 2 seconds @00:53:14.41 AS.reconfigure ASconfig @00:53:14.46 AS.seek 1ml @00:54:12.21 delay 1 second @00:54:13.23 AFV.select :holdingCoil @00:54:13.33 delay 1 second @00:54:14.36 ATV.dial :waste @00:54:15.54 AS.reconfigure ASnorm @00:54:15.58 AS.seek 0.5ml @00:54:41.17 ATV.dial :waste2 @00:54:42.40 AS.seek 0ml @00:55:07.92 AS.coast @00:55:07.96 SS.coast @00:55:08.00 CC.coast @00:55:08.04 CS.coast @00:55:08.08 PC.coast @00:55:08.12 PS.coast @00:55:08.16 Forearm.coast @00:55:08.20 Elbow.coast @00:55:08.60 Gate.power :core,:OFF @00:55:08.61 Gate.power :raw,:OFF @00:55:08.62 Shutdown @00:55:08.64 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @05:14:17.46 Session Begun @05:14:19.87 -> can @05:14:38.86 -> Power @05:15:04.47 -> exit @05:15:04.48 Session Ended @10:00:25.68
Powering core bus @10:00:30.48 Gate.power :raw,:ON @10:00:31.15 SPE.coast @10:00:31.22 SPE.configure SPEconfig @10:00:31.31 AS.configure ASconfig @10:00:31.42 SS.configure SSconfig @10:00:31.52 CC.configure CCconfig @10:00:31.61 CS.configure CSconfig @10:00:31.71 PC.configure PCconfig @10:00:31.81 PS.configure PSconfig @10:00:31.93 Forearm.configure LooseForearm @10:00:32.03 Elbow.configure LooseElbow @10:00:32.15 SC.configure SCloose @10:00:32.26 SE.configure SEconfig @10:00:32.30 CH.coast @10:00:32.36 CH.configure CHconfig @10:00:32.39 PH.coast @10:00:32.45 PH.configure PHconfig @10:00:32.49 PCR.off @10:00:32.53 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:32.56 PCR.caltemp= 2.8814,0.0372,0 @10:00:32.59 All dwarf controllers running firmware version 3.75 @10:00:32.70 Valve.reset @10:00:32.88 PCR.off @10:00:32.91 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:32.94 PCR.caltemp= 2.8814,0.0372,0 @10:00:33.17 Intake.select :close @10:00:33.19 PSV.dial :air @10:00:33.20 Exhaust.select :close @10:00:33.23 CTV.dial :air @10:00:33.40
Forearm.reconfigure LooseForearm @10:00:33.47 CSV.dial :waste1 @10:00:33.49
Forearm.home.seek :retract @10:00:33.61 SSV.dial :spit,avoiding: :exhaust @10:00:33.73 PS.reconfigure PSconfig @10:00:33.80 PS.home.seek 0ml @10:00:34.30 Hand.close @10:00:34.33
Elbow.jog 1500 @10:00:34.46 CS.reconfigure CSfast @10:00:34.49 SS.reconfigure SSconfig @10:00:34.60 CS.home.seek 0ml @10:00:34.64 SS.home.seek 0ml @10:00:34.97
Elbow.reconfigure LooseElbow @10:00:35.03 Elbow.home.seek :clear @10:00:38.46 SE.reconfigure SEconfig @10:00:38.53 SE.home.seek :down @10:00:39.14 SC.reconfigure SCsloppy @10:00:39.19 SC.home.seek :initial @10:00:42.86 PS.coast @10:00:43.39
Forearm.seek :retract @10:00:43.47 Elbow.seek :hammer @10:00:44.80 Forearm.reconfigure TightForearm @10:00:44.85 Forearm.seek 5 + 350 counts @10:00:45.98 Forearm.seek :retract @10:00:47.70 PC.reconfigure PCconfig @10:00:47.81
CC.reconfigure CCconfig @10:00:47.92 PC.home.seek :open @10:00:47.94
CC.home.seek :open @10:00:51.39 Phase [stdphase] @10:00:51.40 GoodNews.email "At 10:00:51 began in tube 3: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:150\n{1 => 22, 2 => 6, 3 => 16}\nCan@10:00:51, 9.0C, 47% humidity, 14.4psia, 13.485V, 0.512A, 0.512A avg, 6.91W",Subject: "Started 20invasivedeployfinal phase stdphase" @10:00:51.42CEST22-May-20
===> STARTED Superphase (phase #12) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @10:00:51.44 Starting initialPurge @10:00:51.46 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:00:51.56 Hand.open @10:00:52.34 Elbow.reconfigure TightElbow @10:00:52.39 Elbow.seek FlushPuck::Garage @10:00:54.34 Elbow.reconfigure LooseElbow @10:00:54.47 Forearm.reconfigure LooseForearm @10:00:54.52 Forearm.seek :garage @10:00:55.53 Hand.close @10:00:56.35 Forearm.seek :retract @10:00:57.39 Elbow.reconfigure TightElbow @10:00:57.46 Elbow.seek CC @10:01:00.58 Elbow.reconfigure LooseElbow @10:01:00.80 Forearm.reconfigure TightForearm @10:01:00.92 Forearm.seek CC @10:01:02.58 CC.seek :closed @10:01:07.03 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.04 @10:01:07.21 Hand.open @10:01:07.99 Forearm.reconfigure LooseForearm @10:01:08.08 Forearm.seek :retract @10:01:09.17 Flushing the Collection Valve::Manifold @10:01:09.45 CSV.dial CSR @10:01:10.99 Pulling 5ml of CSR.flush @10:01:11.01 CSR.select :flush @10:01:11.20 CS.reconfigure CSconfig @10:01:11.29 CS.seek 5ml @10:01:25.07 delay 5 seconds @10:01:30.11 CSR.select :air @10:01:30.41 CS.reconfigure CSfast @10:01:30.50 CS.seek 7.5ml @10:01:37.30 CSV.dial :waste @10:01:38.83 CS.reconfigure CSconfig @10:01:38.93 CS.seek 0ml @10:01:59.10 Purging external Intake @10:01:59.23 CSV.dial :flush @10:01:59.73 CS.reconfigure CSfast @10:01:59.79 CS.seek 10ml @10:02:23.85 delay 5 seconds @10:02:28.88 CSV.dial CBV @10:02:28.92
CBV.dial CSV @10:02:29.74 CTV.dial :intake @10:02:30.53 Intake.select :open @10:02:30.65 CS.seek 0ml @10:02:56.41 Intake.select :close @10:02:56.52 CTV.dial :air @10:02:57.33 Flushing upper manifold @10:02:57.46 CSV.dial :flush @10:02:58.37 CS.seek 5ml @10:03:11.50 delay 5 seconds @10:03:16.53 CSV.dial :air @10:03:17.09 CS.seek 7ml @10:03:22.72 CSV.dial CBV @10:03:22.76
CBV.dial CSV @10:03:23.83 CTV.dial :air @10:03:23.85 CS.seek 0ml @10:03:40.82 delay 7 seconds @10:03:47.86 CS.seek 3ml @10:03:55.69 CSV.dial :waste @10:03:56.30 CS.seek 0ml @10:04:04.28 CSV.dial :flush @10:04:04.76 CS.seek 3ml @10:04:12.60 delay 5 seconds @10:04:17.63 CSV.dial :air @10:04:18.29 CS.seek 5ml @10:04:23.97 CSV.dial :air @10:04:23.99 CS.seek 0ml @10:04:36.41 delay 7 seconds @10:04:43.45 CS.seek 3ml @10:04:51.32 CSV.dial :waste @10:04:52.09 CS.seek 0ml @10:04:59.97 Garaging FlushPuck in Collection Clamp @10:05:00.05 Elbow.reconfigure TightElbow @10:05:00.10 Elbow.seek CC @10:05:00.39 Elbow.reconfigure LooseElbow @10:05:00.49 Forearm.seek CC @10:05:01.49 Hand.close @10:05:02.09 CC.seek :open @10:05:06.45 Forearm.seek :retract @10:05:07.46 Elbow.reconfigure TightElbow @10:05:07.51 Elbow.seek :garage @10:05:10.70 Elbow.reconfigure LooseElbow @10:05:10.80 Forearm.reconfigure TightForearm @10:05:10.85 Forearm.seek :garage @10:05:11.98 Hand.open @10:05:12.66 Forearm.reconfigure LooseForearm @10:05:12.71 Forearm.seek :retract @10:05:13.83 Starting mfb @10:05:13.85 pcrBAC sampling at most 1500ml, wcr at most 1500ml @10:05:13.88 BACfans.email "Started mfb in tube 3 at 10:05:13CEST22-May-20\n{1 => 22, 2 => 6, 3 => 16}\nCan@10:00:51, 9.0C, 47% humidity, 14.4psia, 13.485V, 0.512A, 0.512A avg, 6.91W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @10:05:13.96
Loading sh1 puck from tube 3 into Collection Clamp @10:05:14.09 Readying the MFB @10:05:14.15 Homing MFB Syringe @10:05:14.16 AFV.select :holdingCoil @10:05:14.40 ATV.dial :air2 @10:05:14.43
SC.reconfigure SCtight @10:05:14.53 SC.seek 3 @10:05:15.00 AS.reconfigure ASconfig @10:05:15.05 AS.home.seek :home @10:05:16.72
SC.reconfigure SCloose @10:05:17.03 Elbow.reconfigure TightElbow @10:05:17.08 Elbow.seek 3 @10:05:19.82 Forearm.reconfigure TightForearm @10:05:19.87 Forearm.seek 3 + 700 counts @10:05:20.60 AFV.select :carrierSolution @10:05:20.77 delay 2 seconds @10:05:21.08
SE.reconfigure SEpick @10:05:21.20 SE.seek beamBreak @10:05:22.83 AS.seek 1ml @10:05:23.21
SE.configure SEconfig @10:05:23.60 Elbow.seek 3 @10:05:24.02 Forearm.seek 3 + 300 counts @10:05:24.36 Forearm.reconfigure LooseForearm @10:05:24.45 Hand.close @10:05:25.20 SE.seek :down @10:05:25.80
Forearm.seek :retract @10:05:26.65 SE.coast @10:05:26.84
Elbow.seek CC @10:05:28.66 Elbow.reconfigure LooseElbow @10:05:28.94 Forearm.reconfigure TightForearm @10:05:29.04 Forearm.seek CC @10:05:30.79 CC.seek :closed @10:05:35.24 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.13 @10:05:35.26 Hand.open @10:05:36.16 Forearm.reconfigure LooseForearm @10:05:36.25 Forearm.seek :retract @10:05:37.31 SC.coast @10:05:37.40 collectSample 1500ml w/bubble point of 55psi @10:05:37.42 Priming 7ml @10:05:37.44 Intake.select :close @10:05:37.47 Exhaust.select :close @10:05:37.50 SS.hold @10:05:38.00 Calibrating pressure and temperature sensors @10:05:38.03 CTV.dial :air @10:05:38.06 SSV.dial :spit,avoiding: :exhaust @10:05:38.14 SS.reconfigure SSfast @10:05:38.23 SS.seek 5ml @10:05:41.18 SSV.dial CBV @10:05:41.26
CBV.dial SSV @10:05:42.75 delay 3.8 seconds @10:05:46.86 SSV.dial :spit @10:05:48.33 SS.seek 0ml @10:05:50.87 SSV.dial CBV @10:05:52.34 CTV.dial :intake @10:05:53.22 SSV.dial CBV @10:05:53.32
CBV.dial SSV @10:05:53.35 Allowing 40s for 7.0ml of remaining 7.0ml @10:05:53.37 Intake.select :open @10:05:55.64 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:05:55.67 SS.reconfigure SSinhale.with maxSpeed: 150 @10:05:55.77 SS.seek 7ml @10:05:59.38 Vacuum too high -- waiting up to 40s @10:06:02.11 Intake @30.6psia, vacuum from 20.0 to 24.0psi @10:06:02.15 SS.reconfigure SSinhale.with maxSpeed: 135 @10:06:02.28 SS.seek 7ml @10:06:05.57 Vacuum too high -- waiting up to 40s @10:06:08.13 Intake @30.7psia, vacuum from 20.0 to 24.0psi @10:06:08.17 SS.reconfigure SSinhale.with maxSpeed: 122 @10:06:08.26 SS.seek 7ml @10:06:08.57 Stroke completed -- waiting for pressure to equalize ... @10:06:11.80 Intake.select :close @10:06:12.03 SSV.dial :exhaust @10:06:12.89 Exhaust.select :open @10:06:13.06 SS.reconfigure SSexhale @10:06:13.15 SS.seek 0ml @10:06:16.06 delay 1 second @10:06:16.68
Exhaust.select :close @10:06:16.84 Sampling 1500.0ml @10:06:16.87 Intake.select :close @10:06:16.89 Exhaust.select :close @10:06:16.92 SS.reconfigure SSconfig @10:06:17.00 SS.hold @10:06:17.21 AFV.select :holdingCoil @10:06:17.52
CTV.dial :intake @10:06:17.55 SSV.dial CBV @10:06:17.62
CBV.dial SSV @10:06:17.74 delay 1 second @10:06:18.48
Allowing 130s for 25.0ml of remaining 1500.0ml @10:06:18.51 Intake.select :open @10:06:18.77 ATV.dial :waste @10:06:19.85 AS.reconfigure ASnorm @10:06:19.94 AS.home.seek :empty @10:06:20.78
Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:06:20.82 SS.reconfigure SSinhale.with maxSpeed: 122 @10:06:20.92 SS.seek 25ml @10:06:24.82 Vacuum too high -- waiting up to 40s @10:06:26.91 Intake @30.5psia, vacuum from 20.0 to 24.0psi @10:06:26.92 SS.reconfigure SSinhale.with maxSpeed: 110 @10:06:26.97 SS.seek 25ml @10:06:29.87 Vacuum too high -- waiting up to 40s @10:06:32.43 Intake @30.6psia, vacuum from 20.0 to 24.0psi @10:06:32.45 SS.reconfigure SSinhale.with maxSpeed: 99 @10:06:32.50 SS.seek 25ml @10:06:35.34 Vacuum too high -- waiting up to 40s @10:06:37.59 SS.reconfigure SSinhale.with maxSpeed: 89 @10:06:37.64 SS.seek 25ml @10:06:40.19 Vacuum too high -- waiting up to 40s @10:06:42.33 SS.reconfigure SSinhale.with maxSpeed: 80 @10:06:42.38 SS.seek 25ml @10:06:45.37 Vacuum too high -- waiting up to 40s @10:06:47.70 Intake @30.7psia, vacuum from 20.0 to 24.0psi @10:06:47.71 SS.reconfigure SSinhale.with maxSpeed: 72 @10:06:47.76 SS.seek 25ml @10:06:51.22 Vacuum too high -- waiting up to 40s @10:06:53.30 Intake @30.7psia, vacuum from 20.0 to 24.0psi @10:06:53.31 SS.reconfigure SSinhale.with maxSpeed: 65 @10:06:53.38 SS.seek 25ml @10:06:56.97 Vacuum too high -- waiting up to 40s @10:06:59.04 Intake @30.6psia, vacuum from 20.0 to 24.0psi @10:06:59.06 SS.reconfigure SSinhale.with maxSpeed: 59 @10:06:59.11 SS.seek 25ml @10:07:03.65 Vacuum too high -- waiting up to 40s @10:07:05.97 Intake @30.7psia, vacuum from 20.0 to 24.0psi @10:07:06.62 SS.reconfigure SSinhale.with maxSpeed: 53 @10:07:06.69 SS.seek 25ml @10:07:09.89 Stroke completed -- waiting for pressure to equalize ... @10:07:12.56 ATV.dial :waste @10:07:12.58 ARV.dial between carrier and air1 @10:07:12.66 ASV.dial :waste1 @10:07:14.40
Intake.select :close @10:07:14.56 SSV.dial :exhaust @10:07:15.39 Exhaust.select :open @10:07:15.51 SS.reconfigure SSexhale @10:07:15.55 SS.seek 0ml @10:07:24.52 Exhaust.select :close @10:07:24.64 SSV.dial CBV @10:07:24.66
CBV.dial SSV @10:07:25.49 Allowing 130s for 25.0ml of remaining 1475.0ml @10:07:25.50 Intake.select :open @10:07:27.68 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:07:27.69 SS.reconfigure SSinhale.with maxSpeed: 53 @10:07:27.74 SS.seek 25ml @10:07:33.47 Vacuum too high -- waiting up to 40s @10:07:35.29 Intake @30.7psia, vacuum from 20.0 to 24.0psi @10:07:35.31 SS.reconfigure SSinhale.with maxSpeed: 48 @10:07:35.35 SS.seek 25ml @10:07:41.34 Vacuum too high -- waiting up to 40s @10:07:43.65 Intake @30.8psia, vacuum from 20.0 to 24.0psi @10:07:43.67 SS.reconfigure SSinhale.with maxSpeed: 43 @10:07:43.71 SS.seek 25ml @10:07:50.77 Vacuum too high -- waiting up to 40s @10:07:52.93 SS.reconfigure SSinhale.with maxSpeed: 39 @10:07:52.97 SS.seek 25ml @10:08:00.84 Vacuum too high -- waiting up to 40s @10:08:03.54 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:08:03.56 SS.reconfigure SSinhale.with maxSpeed: 35 @10:08:03.61 SS.seek 25ml @10:08:14.40 Vacuum too high -- waiting up to 40s @10:08:16.76 Intake @30.8psia, vacuum from 20.0 to 24.0psi @10:08:16.78 SS.reconfigure SSinhale.with maxSpeed: 32 @10:08:16.83 SS.seek 25ml @10:08:26.11 Stroke completed -- waiting for pressure to equalize ... @10:08:33.05 Intake.select :close @10:08:33.21 SSV.dial :exhaust @10:08:34.02 Exhaust.select :open @10:08:34.14 SS.reconfigure SSexhale @10:08:34.18 SS.seek 0ml @10:08:43.32 Exhaust.select :close @10:08:43.44 SSV.dial CBV @10:08:43.46
CBV.dial SSV @10:08:44.27 Allowing 130s for 25.0ml of remaining 1450.0ml @10:08:44.28 Intake.select :open @10:08:46.46 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:08:46.47 SS.reconfigure SSinhale.with maxSpeed: 32 @10:08:46.52 SS.seek 25ml @10:08:58.78 Vacuum too high -- waiting up to 40s @10:09:00.85 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:09:00.87 SS.reconfigure SSinhale.with maxSpeed: 29 @10:09:00.91 SS.seek 25ml @10:09:14.81 Vacuum too high -- waiting up to 40s @10:09:17.37 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:09:17.39 SS.reconfigure SSinhale.with maxSpeed: 26 @10:09:17.44 SS.seek 25ml @10:09:37.65 Vacuum too high -- waiting up to 40s @10:09:40.15 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:09:40.17 SS.reconfigure SSinhale.with maxSpeed: 23 @10:09:40.21 SS.seek 25ml @10:10:00.30 Stroke completed -- waiting for pressure to equalize ... @10:10:07.92 Intake.select :close @10:10:08.08 SSV.dial :exhaust @10:10:08.90 Exhaust.select :open @10:10:09.02 SS.reconfigure SSexhale @10:10:09.06 SS.seek 0ml @10:10:18.31 Exhaust.select :close @10:10:18.43 SSV.dial CBV @10:10:18.45
CBV.dial SSV @10:10:19.27 Allowing 130s for 25.0ml of remaining 1425.0ml @10:10:19.28 Intake.select :open @10:10:21.45 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:10:21.47 SS.reconfigure SSinhale.with maxSpeed: 23 @10:10:21.52 SS.seek 25ml @10:11:00.22 Vacuum too high -- waiting up to 40s @10:11:02.54 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:11:02.55 SS.reconfigure SSinhale.with maxSpeed: 21 @10:11:02.60 SS.seek 25ml @10:11:43.98 Stroke completed -- waiting for pressure to equalize ... @10:11:53.39 Intake.select :close @10:11:54.19 SSV.dial :exhaust @10:11:55.02 Exhaust.select :open @10:11:55.14 SS.reconfigure SSexhale @10:11:55.18 SS.seek 0ml @10:12:04.52 Exhaust.select :close @10:12:04.64 SSV.dial CBV @10:12:04.66
CBV.dial SSV @10:12:05.49 Allowing 130s for 25.0ml of remaining 1400.0ml @10:12:05.50 Intake.select :open @10:12:07.68 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:12:07.69 SS.reconfigure SSinhale.with maxSpeed: 21 @10:12:07.75 SS.seek 25ml @10:13:21.75 Vacuum too high -- waiting up to 40s @10:13:24.34 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:13:24.35 SS.reconfigure SSinhale.with maxSpeed: 19 @10:13:24.40 SS.seek 25ml @10:13:35.47 Stroke completed -- waiting for pressure to equalize ... @10:13:43.03 Intake.select :close @10:13:43.19 SSV.dial :exhaust @10:13:43.99 Exhaust.select :open @10:13:44.11 SS.reconfigure SSexhale @10:13:44.15 SS.seek 0ml @10:13:53.38 Exhaust.select :close @10:13:53.50 SSV.dial CBV @10:13:53.52
CBV.dial SSV @10:13:54.34 Allowing 130s for 25.0ml of remaining 1375.0ml @10:13:54.35 Intake.select :open @10:13:56.53 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:13:56.54 SS.reconfigure SSinhale.with maxSpeed: 19 @10:13:56.59 SS.seek 25ml @10:15:28.67 Stroke completed -- waiting for pressure to equalize ... @10:15:38.09 Intake.select :close @10:15:38.84 SSV.dial :exhaust @10:15:39.70 Exhaust.select :open @10:15:39.82 SS.reconfigure SSexhale @10:15:39.86 SS.seek 0ml @10:15:49.06 Exhaust.select :close @10:15:49.17 SSV.dial CBV @10:15:49.21
CBV.dial SSV @10:15:50.01 Allowing 130s for 25.0ml of remaining 1350.0ml @10:15:50.02 Intake.select :open @10:15:52.19 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:15:52.21 SS.reconfigure SSinhale.with maxSpeed: 19 @10:15:52.26 SS.seek 25ml @10:17:24.37 Stroke completed -- waiting for pressure to equalize ... @10:17:34.49 Intake.select :close @10:17:34.65 SSV.dial :exhaust @10:17:35.46 Exhaust.select :open @10:17:35.58 SS.reconfigure SSexhale @10:17:35.62 SS.seek 0ml @10:17:44.64 Exhaust.select :close @10:17:44.76 SSV.dial CBV @10:17:44.78
CBV.dial SSV @10:17:45.62 Allowing 130s for 25.0ml of remaining 1325.0ml @10:17:45.63 Intake.select :open @10:17:47.81 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:17:47.82 SS.reconfigure SSinhale.with maxSpeed: 19 @10:17:47.87 SS.seek 25ml @10:19:20.38 Stroke completed -- waiting for pressure to equalize ... @10:19:31.64 Intake.select :close @10:19:31.80 SSV.dial :exhaust @10:19:32.62 Exhaust.select :open @10:19:32.74 SS.reconfigure SSexhale @10:19:32.78 SS.seek 0ml @10:19:42.07 Exhaust.select :close @10:19:42.19 SSV.dial CBV @10:19:42.21
CBV.dial SSV @10:19:43.04 Allowing 130s for 25.0ml of remaining 1300.0ml @10:19:43.05 Intake.select :open @10:19:45.85 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:19:45.86 SS.reconfigure SSinhale.with maxSpeed: 19 @10:19:45.91 SS.seek 25ml @10:20:42.16 Vacuum too high -- waiting up to 40s @10:20:44.79 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:20:44.81 SS.reconfigure SSinhale.with maxSpeed: 17 @10:20:44.86 SS.seek 25ml @10:21:25.44 Stroke completed -- waiting for pressure to equalize ... @10:21:35.57 Intake.select :close @10:21:35.72 SSV.dial :exhaust @10:21:36.54 Exhaust.select :open @10:21:36.66 SS.reconfigure SSexhale @10:21:36.70 SS.seek 0ml @10:21:45.87 Exhaust.select :close @10:21:45.99 SSV.dial CBV @10:21:46.01
CBV.dial SSV @10:21:46.82 Allowing 130s for 25.0ml of remaining 1275.0ml @10:21:46.83 Intake.select :open @10:21:49.01 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:21:49.02 SS.reconfigure SSinhale.with maxSpeed: 17 @10:21:49.07 SS.seek 25ml @10:23:32.78 Stroke completed -- waiting for pressure to equalize ... @10:23:45.33 Intake.select :close @10:23:45.49 SSV.dial :exhaust @10:23:46.31 Exhaust.select :open @10:23:46.43 SS.reconfigure SSexhale @10:23:46.47 SS.seek 0ml @10:23:55.72 Exhaust.select :close @10:23:55.84 SSV.dial CBV @10:23:55.89
CBV.dial SSV @10:23:56.67 Allowing 130s for 25.0ml of remaining 1250.0ml @10:23:56.68 Intake.select :open @10:23:58.86 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:23:58.87 SS.reconfigure SSinhale.with maxSpeed: 17 @10:23:58.93 SS.seek 25ml @10:25:00.66 Vacuum too high -- waiting up to 40s @10:25:03.49 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:25:03.51 SS.reconfigure SSinhale.with maxSpeed: 15 @10:25:03.56 SS.seek 25ml @10:25:51.08 Stroke completed -- waiting for pressure to equalize ... @10:26:02.37 Intake.select :close @10:26:02.53 SSV.dial :exhaust @10:26:03.36 Exhaust.select :open @10:26:03.48 SS.reconfigure SSexhale @10:26:03.52 SS.seek 0ml @10:26:12.61 Exhaust.select :close @10:26:12.73 SSV.dial CBV @10:26:12.75
CBV.dial SSV @10:26:13.59 Allowing 130s for 25.0ml of remaining 1225.0ml @10:26:13.61 Intake.select :open @10:26:15.78 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:26:15.80 SS.reconfigure SSinhale.with maxSpeed: 15 @10:26:15.85 SS.seek 25ml @10:28:13.21 Stroke completed -- waiting for pressure to equalize ... @10:28:23.62 SS.reconfigure SSconfig @10:28:23.68 SS.hold @10:28:23.81 Intake.select :close @10:28:23.97 ShallowSampler::Clogged in MAIN -- progress too slow after 300.0ml @10:28:23.98 SSV.dial :exhaust @10:28:24.81 Exhaust.select :open @10:28:24.93 SS.reconfigure SSexhale @10:28:24.97 SS.seek 0ml @10:28:34.64 Exhaust.select :close @10:28:34.75 Sampled 300.0ml @10:28:34.77 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:28:34.78 Intake.select :close @10:28:34.85 CSV.dial :waste @10:28:34.94 CS.seek 0ml @10:28:35.02 SSV.dial CBV,avoiding: :exhaust @10:28:35.04
CBV.dial SSV @10:28:35.12 CSV.dial :air @10:28:35.92 CS.seek 10ml @10:28:37.03
CTV.dial :air @10:28:37.85 delay 15 seconds @10:28:52.88 SSV.dial :spit,avoiding: :exhaust @10:28:54.29 CTV.dial CSV,avoiding: :intake @10:28:54.33
SS.seek 0ml @10:28:54.47 SSV.dial CBV,avoiding: :exhaust @10:29:00.63 CBV.dial between SSV and waste2 @10:29:01.05 CTV.dial CSV,avoiding: :air @10:29:01.07
CSV.dial CTV @10:29:01.54 CS.reconfigure CSpressurize @10:29:01.58 CS.seek 0ml @10:29:27.53 BubblePoint: Intake @70.0psia, Outlet @14.5psia @10:29:27.54 CBV.dial SSV @10:29:27.89 SS.reconfigure SSconfig @10:29:27.94 SS.seek 10ml @10:29:33.36 delay 15 seconds @10:29:48.50 Evacuated: Intake @36.6psia, Outlet @0.9psia @10:29:48.51 SSV.dial :spit,avoiding: :exhaust @10:29:49.94 SS.reconfigure SSexhale @10:29:50.00 SS.seek 0ml @10:29:55.12 SS.reconfigure SSfast @10:29:55.17 SS.seek 5ml @10:29:57.72 SSV.dial CBV,avoiding: :exhaust @10:29:57.75
CBV.dial SSV @10:29:59.15 CTV.dial :air,avoiding: :intake @10:30:00.57 CSV.dial CTV @10:30:00.62
SS.seek 10ml @10:30:01.62 CSV.dial :air @10:30:02.11 CS.reconfigure CSfast @10:30:02.16 CS.seek 0ml @10:30:03.10 CS.coast @10:30:03.16
SSV.dial :spit,avoiding: :exhaust @10:30:04.54 SS.reconfigure SSexhale @10:30:04.59 SS.seek 0ml @10:30:09.59 SS.reconfigure SSfast @10:30:09.64 SS.seek 5ml @10:30:12.51 SSV.dial CBV,avoiding: :exhaust @10:30:12.54
CBV.dial SSV @10:30:15.59 SSV.dial :spit,avoiding: :exhaust @10:30:16.98 SS.reconfigure SSconfig @10:30:17.03 SS.seek 0ml @10:30:20.25 SS.coast @10:30:20.30 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:30:20.31 Intake.select :close @10:30:20.39 CSV.dial :waste @10:30:20.54 SSV.dial CBV,avoiding: :exhaust @10:30:20.56
CBV.dial SSV @10:30:21.25 CS.seek 0ml @10:30:21.32 CSV.dial :air @10:30:21.98
CTV.dial :air @10:30:22.00 delay 15 seconds @10:30:22.11 CS.seek 10ml @10:30:37.02
SSV.dial :spit,avoiding: :exhaust @10:30:38.41 CTV.dial CSV,avoiding: :intake @10:30:38.45
SS.reconfigure SSexhale @10:30:38.55 SS.seek 0ml @10:30:38.64 SSV.dial CBV,avoiding: :exhaust @10:30:46.42 CBV.dial between SSV and waste2 @10:30:46.84 CSV.dial between air and CTV @10:30:47.17 CS.seek 8.186ml @10:30:52.39 CTV.dial CSV,avoiding: :air @10:30:52.41
CSV.dial CTV @10:30:52.87 CS.reconfigure CSpressurize @10:30:52.91 CS.seek 0ml @10:31:14.41 CSV.dial :air @10:31:14.90 CS.reconfigure CSfast @10:31:14.95 CS.seek 10ml @10:31:38.91 CSV.dial between air and CTV @10:31:39.27 CS.seek 3.063ml @10:31:56.27 CTV.dial CSV,avoiding: :air @10:31:56.29
CSV.dial CTV @10:31:56.62 CS.reconfigure CSpressurize @10:31:56.66 CS.seek 0ml @10:32:04.22 BubblePoint: Intake @69.1psia, Outlet @13.6psia @10:32:04.23 CBV.dial SSV @10:32:04.58 SS.reconfigure SSconfig @10:32:04.63 SS.seek 10ml @10:32:09.97 delay 15 seconds @10:32:25.11 Evacuated: Intake @36.6psia, Outlet @16.9psia @10:32:25.12 SSV.dial :spit,avoiding: :exhaust @10:32:26.53 SS.reconfigure SSexhale @10:32:26.58 SS.seek 0ml @10:32:31.79 SS.reconfigure SSfast @10:32:31.84 SS.seek 5ml @10:32:34.40 SSV.dial CBV,avoiding: :exhaust @10:32:34.43
CBV.dial SSV @10:32:35.83 CTV.dial :air,avoiding: :intake @10:32:37.30 CSV.dial CTV @10:32:37.36
SS.seek 10ml @10:32:38.36 CSV.dial :air @10:32:38.85 CS.reconfigure CSfast @10:32:38.90 CS.seek 0ml @10:32:39.83
SSV.dial :spit,avoiding: :exhaust @10:32:40.76 CS.coast @10:32:41.22
SS.reconfigure SSexhale @10:32:41.89 SS.seek 0ml @10:32:46.87 SS.reconfigure SSfast @10:32:46.92 SS.seek 5ml @10:32:49.62 SSV.dial CBV,avoiding: :exhaust @10:32:49.64
CBV.dial SSV @10:32:52.07 SSV.dial :spit,avoiding: :exhaust @10:32:53.47 SS.reconfigure SSconfig @10:32:53.53 SS.seek 0ml @10:32:56.89 SS.coast @10:32:56.93 Lysing: Adding 1.5ml lysis @10:32:56.94 CBV.dial between CSV and waste1,avoiding: :DBV @10:32:58.54 CTV.dial CSV @10:32:59.94 CSV.dial CSR @10:33:00.77 Pulling 1ml of CSR.lysis @10:33:00.78 CSR.select :lysis @10:33:00.90 CS.reconfigure CSconfig @10:33:00.94 CS.seek 1ml @10:33:04.48 delay 5 seconds @10:33:09.51 CSR.select :air @10:33:09.69 CS.seek 6ml @10:33:23.41 delay 2 seconds @10:33:25.43 CSV.dial :waste1 @10:33:26.80 CS.reconfigure CSfast @10:33:26.85 CS.seek :empty @10:33:41.51 CSV.dial CSR @10:33:42.93 Pulling 1.5ml of CSR.lysis @10:33:42.94 CSR.select :lysis @10:33:43.06 CS.reconfigure CSconfig @10:33:43.10 CS.seek 1.5ml @10:33:48.01 delay 5 seconds @10:33:53.04 CSR.select :air @10:33:53.22 CS.seek 4.5ml @10:34:01.95 delay 2 seconds @10:34:03.97 CSV.dial CTV @10:34:04.46 CS.seek 1.275ml @10:34:13.77 Heating sh1 puck from tube 3 in Collection Clamp to 85C for 5:00 @10:34:13.79 CH.seek 85C,8:00 @10:37:30.51 CH.hold 5:00,85C @10:42:30.69 Collection Puck Heater at 85C after lysing @10:42:30.71 CS.seek 0ml @10:42:35.03 CTV.dial between spare and air,avoiding: :intake @10:42:36.15 CSV.dial :air @10:42:36.64 CS.seek 5ml @10:42:50.39 CSV.dial CTV @10:42:50.86 delay 2 seconds @10:42:52.88 CSV.dial :air @10:42:53.36 delay 2 seconds @10:42:55.38 CSV.dial CTV @10:42:55.91 delay 2 seconds @10:42:57.93 CSV.dial :air @10:42:58.43 delay 2 seconds @10:43:00.46 CS.seek 0ml @10:43:14.24 Recovering Lysate into Collection syringe @10:43:14.25 CSV.dial CBV @10:43:15.32 CBV.dial CSV @10:43:15.75 CS.seek 4.999ml @10:43:29.55 delay 5 seconds @10:43:34.58 CTV.dial :air @10:43:35.15 CS.seek 7ml @10:43:41.24 delay 5 seconds @10:43:46.27 CSV.dial :air2 @10:43:46.75 CS.seek 10ml @10:43:55.36 CSV.dial between air and CTV,avoiding: CBV @10:43:56.60 Lysing Complete @10:43:56.68 Storing sh1 puck in Collection Clamp to tube 2 @10:43:56.87 SC.reconfigure SCtight @10:43:56.92 SC.seek 2 @10:43:59.08 SC.reconfigure SCloose @10:43:59.31 Elbow.reconfigure TightElbow @10:43:59.36 Elbow.seek 2 @10:44:01.25 Forearm.reconfigure TightForearm @10:44:01.30 Forearm.seek 2 + 700 counts @10:44:02.38 SE.reconfigure SEpick @10:44:02.46 SE.seek beamBreak @10:44:05.10 SE.configure SEconfig @10:44:05.26 Elbow.reconfigure LimpElbow @10:44:05.31 Forearm.reconfigure LooseForearm @10:44:05.36 Forearm.seek 2 @10:44:05.67 Elbow.hold @10:44:05.74 Elbow.reconfigure TightElbow @10:44:05.85 SE.seek 5.07 Pucks below Top Plate @10:44:06.98 Forearm.force -20 @10:44:07.02 Forearm.seek 2 + 332 counts @10:44:07.36 Forearm.reconfigure TightForearm @10:44:07.41 Forearm.seek 2 + 700 counts @10:44:07.70 SE.seek 6.07 Pucks below Top Plate @10:44:08.32 Forearm.reconfigure LooseForearm @10:44:08.37 Forearm.seek :retract @10:44:09.26 Elbow.seek CC @10:44:10.98 Elbow.reconfigure LooseElbow @10:44:11.09 Forearm.seek CC @10:44:12.16 Hand.close @10:44:12.78 CC.seek :open @10:44:17.28 Forearm.seek :retract @10:44:18.34 Elbow.reconfigure TightElbow @10:44:18.39 Elbow.seek 2 @10:44:20.43 Elbow.reconfigure LimpElbow @10:44:20.49 Forearm.seek 2 @10:44:21.53 Elbow.hold @10:44:21.60 Elbow.reconfigure TightElbow @10:44:21.65 Hand.open @10:44:22.42 Forearm.force -20 @10:44:22.46 Forearm.seek 2 + 349 counts @10:44:22.78 Forearm.reconfigure TightForearm @10:44:22.83 Forearm.seek 2 + 700 counts @10:44:23.19 SE.seek :down @10:44:24.35
Forearm.reconfigure LooseForearm @10:44:24.40 Forearm.seek :retract @10:44:25.36 SE.coast @10:44:25.40
SC.coast @10:44:25.44 Tranferring lysate to PRV @10:44:25.45 CSV.dial :air @10:44:25.89 CS.seek 10ml @10:44:25.96 CSV.dial between air and CTV @10:44:26.29 AIV.dial :bypass @10:44:26.38 Beginning samplePassC @10:44:26.39 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:44:26.47 Hand.open @10:44:26.91 Elbow.seek FlushPuck::Garage @10:44:28.95 Elbow.reconfigure LooseElbow @10:44:29.06 Forearm.seek :garage @10:44:30.05 Hand.close @10:44:30.79 Forearm.seek :retract @10:44:31.80 Elbow.reconfigure TightElbow @10:44:31.85 Elbow.seek CC @10:44:35.35 Elbow.reconfigure LooseElbow @10:44:35.46 Forearm.reconfigure TightForearm @10:44:35.51 Forearm.seek CC @10:44:36.86 CC.seek :closed @10:44:41.11 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.01 @10:44:41.19 Hand.open @10:44:41.85 Forearm.reconfigure LooseForearm @10:44:41.90 Forearm.seek :retract @10:44:42.89 AWV.select :air @10:44:42.90 AIV.dial :bypass @10:44:42.91 PSV.dial PSR @10:44:44.34 Pulling 1.5ml of PSR.flush @10:44:44.35 PSR.select :flush @10:44:44.48 PS.seek 1.5ml @10:45:00.56 delay 5 seconds @10:45:05.59 PSV.dial :waste1 @10:45:06.09 PS.seek 0ml @10:45:22.03 PSV.dial PSR @10:45:22.52 PSR.select :air @10:45:22.64 PS.seek 2.5ml @10:45:48.79 PRV.dial CBV @10:45:48.81
CBV.dial PRV @10:45:49.68 PSV.dial :mixloop @10:45:50.86 CTV.dial :air,avoiding: :intake @10:45:50.87 PTV.dial :PRVmixing @10:45:50.96 PS.seek 0ml @10:46:17.16 PSV.dial :air @10:46:17.65 PS.seek 2.5ml @10:46:43.72 PSV.dial :mixloop @10:46:44.20 PS.seek 0ml @10:47:10.32 PSV.dial :air @10:47:10.81 PS.seek 2.5ml @10:47:36.97 PSV.dial :mixloop @10:47:37.45 PS.seek 0ml @10:48:03.54 Garaging FlushPuck in Collection Clamp @10:48:03.62 Elbow.reconfigure TightElbow @10:48:03.67 Elbow.seek CC @10:48:03.93 Elbow.reconfigure LooseElbow @10:48:04.04 Forearm.seek CC @10:48:05.07 Hand.close @10:48:05.66 CC.seek :open @10:48:10.03 Forearm.seek :retract @10:48:11.04 Elbow.reconfigure TightElbow @10:48:11.09 Elbow.seek :garage @10:48:14.27 Elbow.reconfigure LooseElbow @10:48:14.37 Forearm.reconfigure TightForearm @10:48:14.42 Forearm.seek :garage @10:48:15.60 Hand.open @10:48:16.23 Forearm.reconfigure LooseForearm @10:48:16.28 Forearm.seek :retract @10:48:17.33 Connecting PS to CS to transfer sample @10:48:17.34 CBV.dial AIV @10:48:17.90 AIV.dial :bypass @10:48:18.00 PSV.dial :mixloop @10:48:18.01 PTV.dial :PRVmixing @10:48:18.03 PRV.dial AIV @10:48:18.04 CTV.dial :air,avoiding: :intake @10:48:18.06 Loading lyfil puck from tube 3 into Collection Clamp @10:48:18.32 SC.reconfigure SCtight @10:48:18.37 SC.seek 3 @10:48:20.51 SC.reconfigure SCloose @10:48:20.75 Elbow.reconfigure TightElbow @10:48:20.80 Elbow.seek 3 @10:48:22.87 Forearm.reconfigure TightForearm @10:48:22.91 Forearm.seek 3 + 700 counts @10:48:23.97 SE.reconfigure SEpick @10:48:24.05 SE.seek beamBreak @10:48:25.83 SE.configure SEconfig @10:48:26.00 Elbow.seek 3 @10:48:26.26 Forearm.seek 3 + 300 counts @10:48:26.52 Forearm.reconfigure LooseForearm @10:48:26.56 Hand.close @10:48:27.18 SE.seek :down @10:48:27.43
Forearm.seek :retract @10:48:28.38 Elbow.seek CC @10:48:28.67 SE.coast @10:48:30.06
Elbow.reconfigure LooseElbow @10:48:30.16 Forearm.reconfigure TightForearm @10:48:30.21 Forearm.seek CC @10:48:31.63 CC.seek :closed @10:48:35.90 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.10 @10:48:35.91 Hand.open @10:48:36.61 Forearm.reconfigure LooseForearm @10:48:36.66 Forearm.seek :retract @10:48:37.68 SC.coast @10:48:37.71 CTV.dial CSV,avoiding: :intake @10:48:39.13 CSV.dial CTV @10:48:39.46 CS.seek 5ml @10:48:53.24 delay 1:20 @10:50:13.28 CS.seek 2.5ml @10:50:20.62 delay 40 seconds @10:51:00.66 CS.seek 0ml @10:51:08.12 delay 1:00 @10:52:08.15 PSV.dial :air @10:52:08.63 PS.seek 2.5ml @10:52:34.82 PSV.dial :mixloop @10:52:35.29 delay 3 seconds @10:52:38.32 PSV.dial :air @10:52:38.79 delay 2 seconds @10:52:40.81 PSV.dial :mixloop @10:52:41.29 delay 3 seconds @10:52:44.31 PSV.dial :waste1 @10:52:45.66 PS.seek :empty @10:53:11.82 CBV.dial :waste1 @10:53:12.33 delay 5 seconds @10:53:17.36 CBV.dial AIV @10:53:17.87 delay 10 seconds @10:53:27.90 CBV.dial :waste1 @10:53:29.05 delay 10 seconds @10:53:39.08 CBV.dial AIV @10:53:39.59 delay 10 seconds @10:53:49.62 CTV.dial CSV,avoiding: :intake @10:53:49.63 CBV.dial between AIV and waste1 @10:53:50.00 CSV.dial :air @10:53:50.62 CS.seek 10ml @10:54:17.22 CSV.dial CTV @10:54:17.68 CS.seek 0ml @10:54:44.34 CBV.dial :waste1 @10:54:44.67 delay 10 seconds @10:54:54.70 CTV.dial :air,avoiding: :intake @10:54:56.17 Storing lyfil puck in Collection Clamp to tube 2 @10:54:56.36 SC.reconfigure SCtight @10:54:56.41 SC.seek 2 @10:54:58.58 SC.reconfigure SCloose @10:54:58.80 Elbow.reconfigure TightElbow @10:54:58.85 Elbow.seek 2 @10:55:00.74 Forearm.reconfigure TightForearm @10:55:00.80 Forearm.seek 2 + 700 counts @10:55:01.81 SE.reconfigure SEpick @10:55:01.89 SE.seek beamBreak @10:55:04.42 SE.configure SEconfig @10:55:04.58 Elbow.reconfigure LimpElbow @10:55:04.64 Forearm.reconfigure LooseForearm @10:55:04.68 Forearm.seek 2 @10:55:04.97 Elbow.hold @10:55:05.04 Elbow.reconfigure TightElbow @10:55:05.15 SE.seek 6.07 Pucks below Top Plate @10:55:05.68 Forearm.force -20 @10:55:05.73 Forearm.seek 2 + 342 counts @10:55:06.06 Forearm.reconfigure TightForearm @10:55:06.10 Forearm.seek 2 + 700 counts @10:55:06.54 SE.seek 7.07 Pucks below Top Plate @10:55:07.16 Forearm.reconfigure LooseForearm @10:55:07.21 Forearm.seek :retract @10:55:08.14 Elbow.seek CC @10:55:09.84 Elbow.reconfigure LooseElbow @10:55:09.95 Forearm.seek CC @10:55:11.00 Hand.close @10:55:11.67 CC.seek :open @10:55:16.17 Forearm.seek :retract @10:55:17.17 Elbow.reconfigure TightElbow @10:55:17.22 Elbow.seek 2 @10:55:19.10 Elbow.reconfigure LimpElbow @10:55:19.15 Forearm.seek 2 @10:55:20.16 Elbow.hold @10:55:20.23 Elbow.reconfigure TightElbow @10:55:20.28 Hand.open @10:55:21.06 Forearm.force -20 @10:55:21.10 Forearm.seek 2 + 336 counts @10:55:22.05 Forearm.reconfigure TightForearm @10:55:22.09 Forearm.seek 2 + 700 counts @10:55:22.60 SE.seek :down @10:55:23.76
Forearm.reconfigure LooseForearm @10:55:23.81 Forearm.seek :retract @10:55:24.76 SE.coast @10:55:24.82
SC.coast @10:55:24.85 PSV.dial :air @10:55:25.99 PS.seek 0.499ml @10:55:31.95 delay 3 seconds @10:55:34.97 PSV.dial :mixloop @10:55:35.43 PRV.dial :air @10:55:35.92 delay 3 seconds @10:55:38.94 PRV.dial CBV @10:55:39.43 CBV.dial AIV @10:55:39.94 PRV.dial between CBV and air @10:55:40.29 PRV.dial :air @10:55:40.67 PS.reconfigure PSslow @10:55:40.72 PS.seek 0.599ml @10:55:43.20 PRV.dial :waste2 @10:55:43.99 PS.reconfigure PSconfig @10:55:44.04 PS.seek :empty @10:55:51.01 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:55:51.09 Hand.open @10:55:51.43 Elbow.seek FlushPuck::Garage @10:55:53.47 Elbow.reconfigure LooseElbow @10:55:53.59 Forearm.seek :garage @10:55:54.58 Hand.close @10:55:55.39 Forearm.seek :retract @10:55:56.40 Elbow.reconfigure TightElbow @10:55:56.45 Elbow.seek CC @10:55:59.39 Elbow.reconfigure LooseElbow @10:55:59.50 Forearm.reconfigure TightForearm @10:55:59.55 Forearm.seek CC @10:56:00.88 CC.seek :closed @10:56:05.14 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.99 @10:56:05.23 Hand.open @10:56:05.87 Forearm.reconfigure LooseForearm @10:56:05.91 Forearm.seek :retract @10:56:07.00 Clearing PML for modified lysate @10:56:07.02 CTV.dial :air,avoiding: :intake @10:56:07.03 PRV.dial :waste2 @10:56:07.04 PTV.dial :PRV,avoiding: :puck @10:56:07.82 delay 2 seconds @10:56:09.84 PTV.dial :PRVmixing,avoiding: :puck @10:56:10.62 delay 2 seconds @10:56:12.64 PSV.dial :PML @10:56:12.65 delay 2 seconds @10:56:14.68 PRV.dial :air @10:56:15.46 delay 2 seconds @10:56:18.19 PS.seek 1.499ml @10:56:34.20 delay 5 seconds @10:56:39.24 PS.seek 2.5ml @10:56:50.17 PSV.dial :air @10:56:50.66 PS.seek 0ml @10:57:16.73 mixing lysate with speDil @10:57:16.74 PSV.dial :PML @10:57:17.21 CBV.dial :PRV @10:57:17.22 PRV.dial :air @10:57:17.24 delay 2 seconds @10:57:19.26 PRV.dial :speDil @10:57:19.75 delay 2 seconds @10:57:21.84 PS.reconfigure PSslow1 @10:57:21.89 PS.seek 0.225ml @10:57:45.23 delay 5 seconds @10:57:50.26 PRV.dial CBV @10:57:51.06 delay 2 seconds @10:57:53.15 PS.seek 0.475ml @10:58:18.79 delay 5 seconds @10:58:23.82 PRV.dial :air @10:58:24.33 delay 2 seconds @10:58:26.36 PS.seek 0.965ml @10:59:16.30 delay 10 seconds @10:59:26.33 PRV.dial CBV @10:59:26.83 CTV.dial :air,avoiding: :intake @10:59:26.84 PSV.dial :air @10:59:27.31 PS.reconfigure PSconfig @10:59:27.37 PS.seek 2.5ml @10:59:43.83 delay 3 seconds @10:59:46.85 PSV.dial PTV @10:59:47.62 PTV.dial :PRV,avoiding: :puck @10:59:48.43 PS.seek 0ml @11:00:14.55 PSV.dial :air @11:00:15.32 PS.seek 2.5ml @11:00:41.42 delay 3 seconds @11:00:44.44 PSV.dial PTV @11:00:45.22 PTV.dial :PRV,avoiding: :puck @11:00:45.24 PS.seek 0ml @11:01:11.45 Pushing modified lysate into AIV Injection Loop @11:01:11.46 CBV.dial AIV @11:01:11.47 AIV.dial :bypass @11:01:11.49 PRV.dial CBV @11:01:11.50 PSV.dial :air @11:01:12.29 PS.seek 2.5ml @11:01:38.42 delay 3 seconds @11:01:41.44 PSV.dial :PML @11:01:41.92 PTV.dial :PRVmixing,avoiding: :puck @11:01:42.79 PS.reconfigure PSslow1 @11:01:42.84 PS.seek 1.74ml @11:03:00.20 delay 5 seconds @11:03:05.23 AIV.dial :sample @11:03:05.84 AWV.select :waste @11:03:06.01 PS.reconfigure PSslow2 @11:03:06.06 PS.seek 1.22ml @11:04:53.18 delay 15 seconds @11:05:08.21 AIV.dial :bypass @11:05:08.81 PS.reconfigure PSconfig @11:05:08.86 PS.seek 0ml @11:05:22.05 PSV.dial :air @11:05:22.53 PS.seek 2.5ml @11:05:48.64 PSV.dial :PML @11:05:49.12 PS.seek 0ml @11:06:15.33 AIV.dial :coilToMFB @11:06:15.34 AWV.select :air @11:06:15.51 Extracting Nucleic Acid from AIV @11:06:15.54
Beginning samplePassD @11:06:15.61 SPE.hold 2:46:40,55C @11:06:15.68 ASV.dial :SPEdelivery @11:06:15.74
AWV.select :air @11:06:15.75 AIV.dial :bypass @11:06:15.77 PSV.dial PSR @11:06:16.07 delay 20 seconds @11:06:16.94
Pulling 1.5ml of PSR.killMFB @11:06:16.95 PSR.select :killMFB @11:06:17.71 PS.seek 1.5ml @11:06:33.71 delay 5 seconds @11:06:36.10 primeLysateINT @11:06:36.11 AWV.select :air @11:06:36.12 AIV.dial :coilToMFB @11:06:36.14 ATV.dial AIV @11:06:37.93 AS.reconfigure ASslow1 @11:06:37.98 AS.seek 0.299ml @11:06:38.73
PSV.dial :waste1 @11:06:39.25 PS.seek 0ml @11:06:55.24 PSV.dial PSR @11:06:55.74 PSR.select :air @11:06:55.87 PS.seek 2.5ml @11:07:22.02 PRV.dial CBV @11:07:22.05
CBV.dial PRV @11:07:22.07 PSV.dial :mixloop @11:07:23.19 CTV.dial :air,avoiding: :intake @11:07:23.20 PTV.dial :PRVmixing @11:07:23.23 PS.seek 0ml @11:07:38.65 delay 10 seconds @11:07:48.68 ATV.dial :air2 @11:07:49.33
PSV.dial PSR @11:07:49.54 AS.seek 0.329ml @11:07:50.51
Pulling 1.5ml of PSR.flush PSR.select :flush @11:07:50.64 PS.seek 1.5ml @11:07:55.86 ATV.dial :waste2 @11:07:56.43 AS.reconfigure ASnorm @11:07:56.48 AS.seek :empty @11:08:06.83
delay 5 seconds @11:08:11.86 PSV.dial :waste1 @11:08:12.36 PS.seek 0ml @11:08:13.48 Lysate primed! @11:08:13.49 AFV.select :holdingCoil @11:08:13.50 ATV.dial :air2 @11:08:14.14 AS.reconfigure ASslow1 @11:08:14.19 AS.seek 0.069ml @11:08:28.43
PSV.dial PSR @11:08:28.62 delay 3 seconds @11:08:28.93
PSR.select :air @11:08:29.06 PS.seek 2.5ml @11:08:31.65 ATV.dial :AIV @11:08:32.56 AS.seek 0.469ml @11:08:55.24 PRV.dial CBV @11:08:55.27
CBV.dial PRV @11:08:55.28 PSV.dial :mixloop @11:08:56.40 CTV.dial :air,avoiding: :intake @11:08:56.41 PTV.dial :PRVmixing @11:08:56.43 PS.seek 0ml @11:09:22.62 PSV.dial PSR @11:09:23.79 Pulling 1.5ml of PSR.flush @11:09:23.80 PSR.select :flush @11:09:23.93 PS.seek 1.5ml @11:09:40.02 delay 5 seconds @11:09:45.05 PSV.dial :waste1 @11:09:45.56 PS.seek 0ml @11:09:53.31 delay 5 seconds @11:09:58.39 Solid Phase Extraction Heater at 46.1C @11:09:58.40 ATV.dial ASV @11:09:58.81 ASV.dial :SPEdelivery @11:09:58.89 AS.seek 0.041ml @11:10:01.65
PSV.dial PSR @11:10:02.27 PSR.select :air @11:10:02.40 PS.seek 2.5ml @11:10:28.46 PRV.dial CBV @11:10:28.49
CBV.dial PRV @11:10:28.50 PSV.dial :mixloop @11:10:29.60 CTV.dial :air,avoiding: :intake @11:10:29.61 PTV.dial :PRVmixing @11:10:29.63 PS.seek 0ml @11:10:55.74 PSV.dial PSR @11:10:57.01 Pulling 1.5ml of PSR.flush @11:10:57.02 PSR.select :flush @11:10:57.15 PS.seek 1.5ml @11:11:13.21 delay 5 seconds @11:11:18.24 PSV.dial :waste1 @11:11:19.38 PS.seek 0ml @11:11:25.25 delay 10 seconds @11:11:35.28 rinseLysateLineINT @11:11:35.29 AFV.select :carrierSolution @11:11:35.39 delay 2 seconds @11:11:35.43
PSV.dial PSR @11:11:35.93 PSR.select :air @11:11:36.06 PS.seek 2.5ml @11:11:37.43 AS.reconfigure ASconfig @11:11:37.48 AS.seek 1ml @11:12:02.18 PRV.dial CBV @11:12:02.21
CBV.dial PRV @11:12:02.23 PSV.dial :mixloop @11:12:03.32 CTV.dial :air,avoiding: :intake @11:12:03.33 PTV.dial :PRVmixing @11:12:03.35 PS.reconfigure PSslow @11:12:03.40 PS.seek 1.2ml @11:12:30.18 PSV.dial :waste1 @11:12:31.59 PS.reconfigure PSconfig @11:12:31.64 PS.seek 0ml @11:12:32.87 delay 1 second @11:12:33.89 AFV.select :holdingCoil @11:12:33.99 delay 1 second @11:12:35.01 ATV.dial AIV @11:12:35.55 AIV.dial :coilToMFB @11:12:35.56 AWV.select :waste @11:12:35.68 AS.reconfigure ASslow0 @11:12:35.72 AS.seek 0ml @11:12:44.56
PSV.dial :air @11:12:45.79 PS.seek 2.5ml @11:13:11.96 PSV.dial :mixloop @11:13:12.44 PS.reconfigure PSslow @11:13:12.48 PS.seek 0ml @11:14:03.65 PSV.dial :air @11:14:04.13 PS.reconfigure PSconfig @11:14:04.17 PS.seek 2.5ml @11:14:16.56 ATV.dial :air2 @11:14:17.21 AS.reconfigure ASslow1 @11:14:17.26 AS.seek 0.05ml @11:14:27.55 ATV.dial :AIV @11:14:28.37 AS.seek 0.01ml @11:14:30.37
PSV.dial :mixloop @11:14:30.85 PS.reconfigure PSslow @11:14:30.89 PS.seek 0ml @11:14:36.69 ATV.dial :waste @11:14:38.27 AS.seek :empty @11:14:40.73 ethanol wash @11:14:40.74 ATV.dial :air2 @11:14:41.97 AS.reconfigure ASslow2 @11:14:42.03 AS.seek 0.009ml @11:14:52.34 delay 3 seconds @11:14:55.37 Pulling 0.4ml of Ethanol Wash @11:14:55.38 ATV.dial :ethanol @11:14:56.18 AS.reconfigure ASslow1 @11:14:56.23 AS.seek 0.409ml @11:15:22.00
Cleaning Collection Syringe @11:15:22.01 CSV.dial CSR @11:15:22.55 Pulling 4ml of CSR.flush @11:15:22.56 CSR.select :flush @11:15:22.69 CS.seek 4ml @11:15:33.87 delay 5 seconds @11:15:38.90 CSR.select :air @11:15:39.03 CS.seek 10ml @11:15:55.28 CSV.dial CTV @11:15:55.31
CTV.dial CSV @11:15:56.81 CBV.dial :waste1 @11:15:57.33 CS.seek 0ml @11:16:16.99 delay 3 seconds @11:16:20.02 ATV.dial :air1 @11:16:20.41 delay 3 seconds @11:16:23.50 AS.seek 0.434ml @11:16:24.06
CSV.dial CSR @11:16:24.60 Pulling 4ml of CSR.flush @11:16:24.61 CSR.select :flush @11:16:24.74 CS.seek 4ml @11:16:28.77 delay 3 seconds @11:16:31.80 ATV.dial :ethanol @11:16:32.38 AS.seek 0.419ml @11:16:35.67 delay 3 seconds @11:16:35.90
delay 5 seconds @11:16:38.70 ATV.dial ASV @11:16:40.01 AS.seek 0.092ml @11:16:41.53
CSR.select :air @11:16:41.65 CS.seek 10ml @11:16:57.93 CSV.dial CTV @11:16:57.97
CTV.dial CSV @11:16:58.46 CBV.dial :waste1 @11:16:58.48 CS.seek 0ml @11:17:25.22 Garaging FlushPuck in Collection Clamp @11:17:25.30 Elbow.reconfigure TightElbow @11:17:25.34 Elbow.seek CC @11:17:25.59 Elbow.reconfigure LooseElbow @11:17:25.69 Forearm.seek CC @11:17:26.68 Hand.close @11:17:27.35 CC.seek :open @11:17:31.72 Forearm.seek :retract @11:17:32.74 Elbow.reconfigure TightElbow @11:17:32.79 Elbow.seek :garage @11:17:35.96 Elbow.reconfigure LooseElbow @11:17:36.07 Forearm.reconfigure TightForearm @11:17:36.12 Forearm.seek :garage @11:17:37.28 Hand.open @11:17:38.03 Forearm.reconfigure LooseForearm @11:17:38.08 Forearm.seek :retract @11:17:39.09 Whole Cell Archive sampling 1500ml @11:17:39.11 Loading wcr puck from tube 3 into Collection Clamp @11:17:39.37 SC.reconfigure SCtight @11:17:39.42 SC.seek 3 @11:17:41.59 SC.reconfigure SCloose @11:17:41.82 Elbow.reconfigure TightElbow @11:17:41.87 Elbow.seek 3 @11:17:43.98 Forearm.reconfigure TightForearm @11:17:44.03 Forearm.seek 3 + 700 counts @11:17:45.08 SE.reconfigure SEpick @11:17:45.16 SE.seek beamBreak @11:17:46.03 delay 3 seconds @11:17:47.14 SE.configure SEconfig @11:17:47.31 Elbow.seek 3 @11:17:47.71 Forearm.seek 3 + 300 counts @11:17:47.93 Forearm.reconfigure LooseForearm @11:17:47.98 Hand.close @11:17:48.61 SE.seek :down @11:17:48.84 Forearm.seek :retract @11:17:49.05 ATV.dial :waste2 @11:17:49.78 AS.reconfigure ASconfig @11:17:49.84 AS.seek :empty @11:17:49.91 Elbow.seek CC @11:17:50.17 SE.coast @11:17:51.52 Elbow.reconfigure LooseElbow @11:17:52.26 Forearm.reconfigure TightForearm @11:17:52.33 Forearm.seek CC @11:17:53.68 CC.seek :closed @11:17:55.48 ATV.dial :air2 @11:17:55.92 AS.reconfigure ASslow1 @11:17:56.00 AS.seek 1ml @11:17:57.94 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.06 @11:17:57.95 Hand.open @11:17:58.67 Forearm.reconfigure LooseForearm @11:17:58.72 Forearm.seek :retract @11:17:59.73 SC.coast @11:17:59.77 collectSample 1500ml w/bubble point of 55psi @11:17:59.79 Priming 7ml @11:17:59.80 Intake.select :close @11:17:59.81 Exhaust.select :close @11:17:59.82 SS.hold @11:18:00.05 Calibrating pressure sensors @11:18:00.06 CTV.dial :air @11:18:01.48 SSV.dial :spit,avoiding: :exhaust @11:18:01.50 SS.reconfigure SSfast @11:18:01.56 SS.seek 5ml @11:18:04.22 SSV.dial CBV @11:18:04.25 CBV.dial SSV @11:18:05.78 delay 3.8 seconds @11:18:09.73 SSV.dial :spit @11:18:11.17 SS.seek 0ml @11:18:13.79 SSV.dial CBV @11:18:15.20 CTV.dial :intake @11:18:16.01 SSV.dial CBV @11:18:16.04 CBV.dial SSV @11:18:16.06 Allowing 40s for 7.0ml of remaining 7.0ml @11:18:16.07 Intake.select :open @11:18:18.25 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:18:18.27 SS.reconfigure SSinhale.with maxSpeed: 150 @11:18:18.31 SS.seek 7ml @11:18:22.49 Vacuum too high -- waiting up to 40s @11:18:26.12 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:18:26.14 SS.reconfigure SSinhale.with maxSpeed: 135 @11:18:26.19 SS.seek 7ml @11:18:28.76 Stroke completed -- waiting for pressure to equalize ... @11:18:34.58 Intake.select :close @11:18:34.74 SSV.dial :exhaust @11:18:35.56 Exhaust.select :open @11:18:35.69 SS.reconfigure SSexhale @11:18:35.74 SS.seek 0ml @11:18:39.61 Exhaust.select :close @11:18:39.72 Sampling 1500.0ml @11:18:39.74 Intake.select :close @11:18:39.75 Exhaust.select :close @11:18:39.76 SS.reconfigure SSconfig @11:18:39.81 SS.hold @11:18:40.04 CTV.dial :intake @11:18:40.07 SSV.dial CBV @11:18:40.09 CBV.dial SSV @11:18:40.95 Allowing 130s for 25.0ml of remaining 1500.0ml @11:18:40.96 Intake.select :open @11:18:43.15 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:18:43.17 SS.reconfigure SSinhale.with maxSpeed: 135 @11:18:43.21 SS.seek 25ml @11:18:46.74 Vacuum too high -- waiting up to 40s @11:18:49.11 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:18:49.13 SS.reconfigure SSinhale.with maxSpeed: 122 @11:18:49.18 SS.seek 25ml @11:18:52.21 Vacuum too high -- waiting up to 40s @11:18:54.62 SS.reconfigure SSinhale.with maxSpeed: 110 @11:18:54.67 SS.seek 25ml @11:18:57.63 Vacuum too high -- waiting up to 40s @11:19:00.01 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:19:00.03 SS.reconfigure SSinhale.with maxSpeed: 99 @11:19:00.08 SS.seek 25ml @11:19:03.33 Vacuum too high -- waiting up to 40s @11:19:05.63 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:19:05.65 SS.reconfigure SSinhale.with maxSpeed: 89 @11:19:05.70 SS.seek 25ml @11:19:08.83 Vacuum too high -- waiting up to 40s @11:19:10.95 Intake @31.1psia, vacuum from 20.0 to 24.0psi @11:19:10.97 SS.reconfigure SSinhale.with maxSpeed: 80 @11:19:11.02 SS.seek 25ml @11:19:14.40 Vacuum too high -- waiting up to 40s @11:19:16.51 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:19:16.53 SS.reconfigure SSinhale.with maxSpeed: 72 @11:19:16.58 SS.seek 25ml @11:19:20.24 Vacuum too high -- waiting up to 40s @11:19:22.11 Intake @31.1psia, vacuum from 20.0 to 24.0psi @11:19:22.13 SS.reconfigure SSinhale.with maxSpeed: 65 @11:19:22.17 SS.seek 25ml @11:19:26.22 Stroke completed -- waiting for pressure to equalize ... @11:19:30.73 Intake.select :close @11:19:30.89 SSV.dial :exhaust @11:19:31.72 Exhaust.select :open @11:19:31.84 SS.reconfigure SSexhale @11:19:31.89 SS.seek 0ml @11:19:41.62 Exhaust.select :close @11:19:41.74 SSV.dial CBV @11:19:41.76 CBV.dial SSV @11:19:42.59 Allowing 130s for 25.0ml of remaining 1475.0ml @11:19:42.60 Intake.select :open @11:19:44.79 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:19:44.81 SS.reconfigure SSinhale.with maxSpeed: 65 @11:19:44.85 SS.seek 25ml @11:19:50.60 Vacuum too high -- waiting up to 40s @11:19:52.39 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:19:52.41 SS.reconfigure SSinhale.with maxSpeed: 59 @11:19:52.45 SS.seek 25ml @11:19:57.64 Vacuum too high -- waiting up to 40s @11:19:59.52 SS.reconfigure SSinhale.with maxSpeed: 53 @11:19:59.57 SS.seek 25ml @11:20:05.71 Vacuum too high -- waiting up to 40s @11:20:07.58 SS.reconfigure SSinhale.with maxSpeed: 48 @11:20:07.63 SS.seek 25ml @11:20:16.42 Vacuum too high -- waiting up to 40s @11:20:18.32 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:20:18.34 SS.reconfigure SSinhale.with maxSpeed: 43 @11:20:18.38 SS.seek 25ml @11:20:29.85 Stroke completed -- waiting for pressure to equalize ... @11:20:34.99 Intake.select :close @11:20:35.15 SSV.dial :exhaust @11:20:35.98 Exhaust.select :open @11:20:36.10 SS.reconfigure SSexhale @11:20:36.15 SS.seek 0ml @11:20:45.28 Exhaust.select :close @11:20:45.40 SSV.dial CBV @11:20:45.42 CBV.dial SSV @11:20:46.28 Allowing 130s for 25.0ml of remaining 1450.0ml @11:20:46.29 Intake.select :open @11:20:48.47 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:20:48.49 SS.reconfigure SSinhale.with maxSpeed: 43 @11:20:48.54 SS.seek 25ml @11:21:06.21 Vacuum too high -- waiting up to 40s @11:21:07.81 Intake @31.0psia, vacuum from 20.0 to 24.0psi @11:21:07.83 SS.reconfigure SSinhale.with maxSpeed: 39 @11:21:07.88 SS.seek 25ml @11:21:17.60 delay 5 seconds @11:21:22.63 ATV.dial ASV @11:21:23.60 ASV.dial :SPEdelivery @11:21:23.62 AS.seek 0.02ml @11:21:35.03 Stroke completed -- waiting for pressure to equalize ... @11:21:40.83 Intake.select :close @11:21:41.00 SSV.dial :exhaust @11:21:41.83 Exhaust.select :open @11:21:41.95 SS.reconfigure SSexhale @11:21:42.00 SS.seek 0ml @11:21:51.12 Exhaust.select :close @11:21:51.24 SSV.dial CBV @11:21:51.26 CBV.dial SSV @11:21:52.11 Allowing 130s for 25.0ml of remaining 1425.0ml @11:21:52.12 Intake.select :open @11:21:54.31 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:21:54.33 SS.reconfigure SSinhale.with maxSpeed: 39 @11:21:54.37 SS.seek 25ml @11:22:29.20 Vacuum too high -- waiting up to 40s @11:22:31.07 Intake @31.1psia, vacuum from 20.0 to 24.0psi @11:22:31.09 SS.reconfigure SSinhale.with maxSpeed: 35 @11:22:31.14 SS.seek 25ml @11:22:43.04 Stroke completed -- waiting for pressure to equalize ... @11:22:48.19 Intake.select :close @11:22:48.35 SSV.dial :exhaust @11:22:49.17 Exhaust.select :open @11:22:49.29 SS.reconfigure SSexhale @11:22:49.34 SS.seek 0ml @11:22:58.48 Exhaust.select :close @11:22:58.60 SSV.dial CBV @11:22:58.62 CBV.dial SSV @11:22:59.45 Allowing 130s for 25.0ml of remaining 1400.0ml @11:22:59.46 Intake.select :open @11:23:01.65 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:23:01.67 SS.reconfigure SSinhale.with maxSpeed: 35 @11:23:01.71 SS.seek 25ml @11:23:52.04 Stroke completed -- waiting for pressure to equalize ... @11:23:58.47 Intake.select :close @11:23:58.63 SSV.dial :exhaust @11:23:59.45 Exhaust.select :open @11:23:59.57 SS.reconfigure SSexhale @11:23:59.62 SS.seek 0ml @11:24:08.61 Exhaust.select :close @11:24:08.72 SSV.dial CBV @11:24:08.76 CBV.dial SSV @11:24:09.61 Allowing 130s for 25.0ml of remaining 1375.0ml @11:24:09.62 Intake.select :open @11:24:11.81 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:24:11.83 SS.reconfigure SSinhale.with maxSpeed: 35 @11:24:11.87 SS.seek 25ml @11:24:40.99 delay 5 seconds @11:24:46.02 ATV.dial :air2 @11:24:47.05 AS.seek 0.52ml @11:25:00.11 Vacuum too high -- waiting up to 40s @11:25:02.24 Intake @31.0psia, vacuum from 20.0 to 24.0psi @11:25:02.26 SS.reconfigure SSinhale.with maxSpeed: 32 @11:25:02.30 SS.seek 25ml @11:25:05.86 Stroke completed -- waiting for pressure to equalize ... @11:25:10.34 Intake.select :close @11:25:10.50 SSV.dial :exhaust @11:25:11.34 Exhaust.select :open @11:25:11.46 SS.reconfigure SSexhale @11:25:11.51 SS.seek 0ml @11:25:20.47 Exhaust.select :close @11:25:20.59 SSV.dial CBV @11:25:20.61 CBV.dial SSV @11:25:21.47 Allowing 130s for 25.0ml of remaining 1350.0ml @11:25:21.48 Intake.select :open @11:25:23.67 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:25:23.69 SS.reconfigure SSinhale.with maxSpeed: 32 @11:25:23.73 SS.seek 25ml @11:26:18.96 Stroke completed -- waiting for pressure to equalize ... @11:26:25.36 Intake.select :close @11:26:25.52 SSV.dial :exhaust @11:26:26.34 Exhaust.select :open @11:26:26.46 SS.reconfigure SSexhale @11:26:26.51 SS.seek 0ml @11:26:27.90 delay 5 seconds @11:26:32.93 ATV.dial ASV @11:26:34.53 ASV.dial :wasteR @11:26:34.91 AS.seek 0.3ml @11:26:35.50 Exhaust.select :close @11:26:35.61 SSV.dial CBV @11:26:35.65 CBV.dial SSV @11:26:36.47 Allowing 130s for 25.0ml of remaining 1325.0ml @11:26:36.48 Intake.select :open @11:26:38.66 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:26:38.68 SS.reconfigure SSinhale.with maxSpeed: 32 @11:26:38.73 SS.seek 25ml @11:27:19.49 delay 20 seconds @11:27:24.96 Vacuum too high -- waiting up to 40s @11:27:27.33 Intake @31.0psia, vacuum from 20.0 to 24.0psi @11:27:27.35 SS.reconfigure SSinhale.with maxSpeed: 29 @11:27:27.39 SS.seek 25ml @11:27:37.49 Stroke completed -- waiting for pressure to equalize ... @11:27:39.52 AS.seek 0.07ml @11:27:43.29 Intake.select :close @11:27:43.45 SSV.dial :exhaust @11:27:44.28 Exhaust.select :open @11:27:44.40 SS.reconfigure SSexhale @11:27:44.45 SS.seek 0ml @11:27:53.97 Exhaust.select :close @11:27:54.09 SSV.dial CBV @11:27:54.11 CBV.dial SSV @11:27:54.97 Allowing 130s for 25.0ml of remaining 1300.0ml @11:27:54.98 Intake.select :open @11:27:57.16 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:27:57.18 SS.reconfigure SSinhale.with maxSpeed: 29 @11:27:57.23 SS.seek 25ml @11:28:26.06 delay 20 seconds @11:28:46.09 ATV.dial :waste2 @11:28:46.92 AS.reconfigure ASconfig @11:28:46.97 AS.seek :empty @11:28:51.33 Elution @11:28:51.34 ATV.dial :air1 @11:28:52.44 AS.reconfigure ASslow1 @11:28:52.49 AS.seek 0.049ml @11:28:57.59 Stroke completed -- waiting for pressure to equalize ... @11:29:02.87 delay 3 seconds @11:29:06.48 ATV.dial :ElutionWater @11:29:06.54 Intake.select :close @11:29:06.70 SSV.dial :exhaust @11:29:07.16 AS.seek 0.109ml @11:29:07.53 Exhaust.select :open @11:29:07.66 SS.reconfigure SSexhale @11:29:07.72 SS.seek 0ml @11:29:17.11 Exhaust.select :close @11:29:17.23 SSV.dial CBV @11:29:17.25 CBV.dial SSV @11:29:18.09 Allowing 130s for 25.0ml of remaining 1275.0ml @11:29:18.10 Intake.select :open @11:29:19.49 delay 3 seconds @11:29:20.29 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:29:20.31 SS.reconfigure SSinhale.with maxSpeed: 29 @11:29:20.35 SS.seek 25ml @11:29:22.52 ATV.dial ASV,via: :air2 @11:29:24.52 ASV.dial :SPEelution @11:29:25.10 AFV.select :carrierSolution @11:29:25.26 AS.reconfigure ASnorm @11:29:25.32 AS.seek 0.759ml @11:29:58.42 delay 3 seconds @11:29:59.27 Vacuum too high -- waiting up to 40s @11:30:01.44 AFV.select :holdingCoil @11:30:01.72 Intake @31.0psia, vacuum from 20.0 to 24.0psi @11:30:01.76 AS.reconfigure ASslow1 @11:30:01.79 SS.reconfigure SSinhale.with maxSpeed: 26 @11:30:01.87 AS.seek 0.474ml @11:30:01.94 SS.seek 25ml @11:30:26.43 Stroke completed -- waiting for pressure to equalize ... @11:30:34.10 Intake.select :close @11:30:34.26 SSV.dial :exhaust @11:30:35.08 Exhaust.select :open @11:30:35.20 SS.reconfigure SSexhale @11:30:35.25 SS.seek 0ml @11:30:44.72 Exhaust.select :close @11:30:44.84 SSV.dial CBV @11:30:44.86 CBV.dial SSV @11:30:45.72 Allowing 130s for 25.0ml of remaining 1250.0ml @11:30:45.73 Intake.select :open @11:30:47.92 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:30:47.93 SS.reconfigure SSinhale.with maxSpeed: 26 @11:30:47.98 SS.seek 25ml @11:30:59.57 delay 5 seconds @11:31:04.67 AS.reconfigure ASslow2 @11:31:04.72 AS.seek 0.439ml @11:31:38.90 Vacuum too high -- waiting up to 40s @11:31:40.37 delay 1:00 @11:31:41.91 Intake @31.0psia, vacuum from 20.0 to 24.0psi @11:31:41.93 SS.reconfigure SSinhale.with maxSpeed: 23 @11:31:41.98 SS.seek 25ml @11:32:01.02 Stroke completed -- waiting for pressure to equalize ... @11:32:09.33 Intake.select :close @11:32:09.49 SSV.dial :exhaust @11:32:10.31 Exhaust.select :open @11:32:10.43 SS.reconfigure SSexhale @11:32:10.48 SS.seek 0ml @11:32:19.80 Exhaust.select :close @11:32:19.92 SSV.dial CBV @11:32:19.94 CBV.dial SSV @11:32:20.78 Allowing 130s for 25.0ml of remaining 1225.0ml @11:32:20.79 Intake.select :open @11:32:22.98 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:32:23.00 SS.reconfigure SSinhale.with maxSpeed: 23 @11:32:23.04 SS.seek 25ml @11:32:40.39 pushing eluate to holding coil @11:32:40.46 AS.seek 0.279ml @11:33:28.74 Vacuum too high -- waiting up to 40s @11:33:31.90 Intake @31.1psia, vacuum from 20.0 to 24.0psi @11:33:31.92 SS.reconfigure SSinhale.with maxSpeed: 21 @11:33:31.97 SS.seek 25ml @11:33:44.38 Stroke completed -- waiting for pressure to equalize ... @11:33:53.36 Intake.select :close @11:33:53.52 SSV.dial :exhaust @11:33:54.34 Exhaust.select :open @11:33:54.47 SS.reconfigure SSexhale @11:33:54.52 SS.seek 0ml @11:34:03.56 Exhaust.select :close @11:34:03.69 SSV.dial CBV @11:34:03.71 CBV.dial SSV @11:34:04.56 Allowing 130s for 25.0ml of remaining 1200.0ml @11:34:04.57 Intake.select :open @11:34:06.76 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:34:06.78 SS.reconfigure SSinhale.with maxSpeed: 21 @11:34:06.82 SS.seek 25ml @11:35:08.37 Vacuum too high -- waiting up to 40s @11:35:11.80 Intake @31.1psia, vacuum from 20.0 to 24.0psi @11:35:11.81 SS.reconfigure SSinhale.with maxSpeed: 19 @11:35:11.86 SS.seek 25ml @11:35:21.50 delay 30 seconds @11:35:36.90 Stroke completed -- waiting for pressure to equalize ... @11:35:47.69 Intake.select :close @11:35:47.85 SSV.dial :exhaust @11:35:48.67 Exhaust.select :open @11:35:48.79 SS.reconfigure SSexhale @11:35:48.84 SS.seek 0ml @11:35:51.53 ATV.dial :waste,via: :air3 @11:35:53.17 AS.reconfigure ASconfig @11:35:53.22 AS.seek :empty @11:35:57.80 Exhaust.select :close @11:35:57.92 SSV.dial CBV @11:35:57.94 CBV.dial SSV @11:35:58.80 Allowing 130s for 25.0ml of remaining 1175.0ml @11:35:58.81 Intake.select :open @11:36:01.67 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:36:01.69 SS.reconfigure SSinhale.with maxSpeed: 19 @11:36:01.73 SS.seek 25ml @11:36:09.62 Parking Bleach in LysateSource line and SPEcolumn @11:36:09.63 ATV.dial :air3 @11:36:10.46 AS.reconfigure ASslow1 @11:36:10.51 AS.seek 0.029ml @11:36:16.89 ATV.dial :ElutionWater @11:36:17.50 AS.seek 0.059ml @11:36:23.78 ATV.dial :air3 @11:36:24.40 AS.seek 0.069ml @11:36:26.67 delay 2 seconds @11:36:28.69 ATV.dial :bleach @11:36:29.17 AS.seek 0.769ml @11:37:03.24 Vacuum too high -- waiting up to 40s @11:37:07.40 Intake @31.1psia, vacuum from 20.0 to 24.0psi @11:37:07.42 SS.reconfigure SSinhale.with maxSpeed: 17 @11:37:07.47 SS.seek 25ml @11:37:43.06 Stroke completed -- waiting for pressure to equalize ... @11:37:57.01 Intake.select :close @11:37:57.16 SSV.dial :exhaust @11:37:57.99 Exhaust.select :open @11:37:58.11 SS.reconfigure SSexhale @11:37:58.16 SS.seek 0ml @11:38:07.25 Exhaust.select :close @11:38:08.06 SSV.dial CBV @11:38:08.08 CBV.dial SSV @11:38:08.94 Allowing 130s for 25.0ml of remaining 1150.0ml @11:38:08.95 Intake.select :open @11:38:11.13 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:38:11.15 SS.reconfigure SSinhale.with maxSpeed: 17 @11:38:11.20 SS.seek 25ml @11:38:50.19 delay 3 seconds @11:38:53.22 ATV.dial :air3 @11:38:53.76 delay 3 seconds @11:38:56.85 AS.seek 0.794ml @11:39:02.18 delay 3 seconds @11:39:05.05 Vacuum too high -- waiting up to 40s @11:39:05.29 ATV.dial :bleach @11:39:05.97 AS.seek 0.779ml @11:39:09.12 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:39:09.14 SS.reconfigure SSinhale.with maxSpeed: 15 @11:39:09.19 SS.seek 25ml @11:39:09.26 delay 3 seconds @11:39:12.29 ATV.dial :AIV @11:39:13.53 AS.reconfigure ASconfig @11:39:13.58 AS.seek 0.015ml @11:39:57.81 ATV.dial :waste @11:39:59.23 AS.seek :empty @11:40:00.44 ATV.dial :air3 @11:40:01.12 AS.reconfigure ASslow1 @11:40:01.17 AS.seek 0.009ml @11:40:03.48 delay 2 seconds @11:40:05.50 ATV.dial :bleach @11:40:05.96 AS.seek 0.579ml @11:40:06.03 Stroke completed -- waiting for pressure to equalize ... @11:40:19.65 SS.reconfigure SSconfig @11:40:19.70 SS.hold @11:40:19.83 Intake.select :close @11:40:20.00 ShallowSampler::Clogged in WCR -- progress too slow after 375.0ml @11:40:20.02 SSV.dial :exhaust @11:40:20.85 Exhaust.select :open @11:40:20.97 SS.reconfigure SSexhale @11:40:21.02 SS.seek 0ml @11:40:30.50 Exhaust.select :close @11:40:30.61 Sampled 375.0ml @11:40:30.63 Drying puck before fixing for 4:00 @11:40:30.64 CBV.dial :waste2b @11:40:31.46 delay 1:00 @11:41:31.50 CSV.dial :air @11:41:31.98 CS.reconfigure CSfast @11:41:32.04 CS.seek 10ml @11:41:56.49 Pumping wcr puck from tube 3 up to 55psi... @11:41:56.51 CBV.dial SSV @11:41:57.91 CBV.dial between CSV and waste2b @11:41:58.97 CSV.dial between air and CTV @11:41:59.33 CS.seek 7.202ml @11:42:00.88 delay 3 seconds @11:42:03.90 ATV.dial :air3 @11:42:04.44 delay 3 seconds @11:42:06.94 CTV.dial CSV,avoiding: :air @11:42:06.96 CSV.dial CTV @11:42:07.52 AS.seek 0.604ml @11:42:07.85 CS.reconfigure CSpressurize @11:42:07.90 CS.seek 0ml @11:42:12.83 delay 3 seconds @11:42:15.86 ATV.dial :bleach @11:42:16.46 AS.seek 0.589ml @11:42:19.73 delay 3 seconds @11:42:22.76 ATV.dial :ASV @11:42:23.75 ASV.dial :wasteR @11:42:24.34 AS.seek 0ml @11:42:26.96 CSV.dial :air @11:42:27.46 CS.reconfigure CSfast @11:42:27.52 CS.seek 10ml @11:42:51.81 CSV.dial between air and CTV @11:42:52.16 CS.seek 2.539ml @11:43:10.85 CTV.dial CSV,avoiding: :air @11:43:10.87 CSV.dial CTV @11:43:11.22 CS.reconfigure CSpressurize @11:43:11.27 CS.seek 0ml @11:43:15.70 CBV.dial :waste2b @11:43:16.07 CS.reconfigure CSconfig @11:43:16.13 CS.seek :empty @11:43:19.74 delay 1:00 @11:44:19.77 CTV.dial :air,avoiding: :intake @11:44:21.18 Fix #1 of 2 (with rnal) for 2:00 @11:44:21.20 CSV.dial CSR @11:44:21.75 Pulling 2ml of CSR.rnal @11:44:21.76 CSR.select :rnal @11:44:21.88 CS.seek 2ml @11:44:23.05 delay 1:00 @11:44:28.06 delay 5 seconds @11:44:33.09 CSR.select :air @11:44:33.21 CS.reconfigure CSfast @11:44:33.26 CS.seek 10ml @11:44:52.93 Pumping wcr puck from tube 3 up to 55psi... @11:44:52.95 CBV.dial SSV @11:44:54.96 CBV.dial between CSV and waste2b @11:44:56.00 CSV.dial between air and CTV @11:44:56.68 CS.seek 7.202ml @11:45:04.35 CTV.dial CSV,avoiding: :air @11:45:04.38 CSV.dial CTV @11:45:05.86 CS.reconfigure CSpressurize @11:45:05.91 CS.seek 0ml @11:45:23.08 AFV.select :carrierSolution @11:45:23.18 delay 2 seconds @11:45:24.97 CSV.dial :air @11:45:25.20 AS.reconfigure ASconfig @11:45:25.25 AS.seek 1ml @11:45:25.48 CS.reconfigure CSfast @11:45:25.53 CS.seek 10ml @11:45:49.82 CSV.dial between air and CTV @11:45:50.18 CS.seek 2.204ml @11:46:09.44 CTV.dial CSV,avoiding: :air @11:46:09.46 CSV.dial CTV @11:46:09.80 CS.reconfigure CSpressurize @11:46:09.85 CS.seek 0ml @11:46:11.99 delay 2:00 @11:46:23.02 delay 1 second @11:46:24.04 AFV.select :holdingCoil @11:46:24.14 delay 1 second @11:46:25.17 ATV.dial ASV @11:46:25.18 ASV.dial :wasteR @11:46:25.20 AS.reconfigure ASslow1 @11:46:25.24 AS.seek 0ml @11:48:12.02 CBV.dial :waste2b @11:48:12.37 CS.reconfigure CSconfig @11:48:12.42 CS.seek :empty @11:48:17.42 delay 1:00 @11:49:17.45 CTV.dial :air,avoiding: :intake @11:49:18.86 Fix #2 of 2 (with rnal) for 2:00 @11:49:18.88 CSV.dial CSR @11:49:19.42 Pulling 2ml of CSR.rnal @11:49:19.44 CSR.select :rnal @11:49:19.56 CS.seek 2ml @11:49:25.66 delay 5 seconds @11:49:30.69 CSR.select :air @11:49:30.82 CS.reconfigure CSfast @11:49:30.87 CS.seek 10ml @11:49:46.60 delay 20 seconds @11:49:50.44 Pumping wcr puck from tube 3 up to 55psi... @11:49:50.46 CBV.dial SSV @11:49:51.85 CBV.dial between CSV and waste2b @11:49:52.90 CSV.dial between air and CTV @11:49:53.57 CS.seek 7.193ml @11:50:01.20 CTV.dial CSV,avoiding: :air @11:50:01.23 CSV.dial CTV @11:50:02.70 CS.reconfigure CSpressurize @11:50:02.75 CS.seek 0ml @11:50:06.62 ATV.dial :air3 @11:50:08.54 AS.seek 0.59ml @11:50:21.78 CSV.dial :air @11:50:22.28 CS.reconfigure CSfast @11:50:22.34 CS.seek 10ml @11:50:46.70 CSV.dial between air and CTV @11:50:47.06 CS.seek 2.176ml @11:51:06.42 CTV.dial CSV,avoiding: :air @11:51:06.44 CSV.dial CTV @11:51:06.78 CS.reconfigure CSpressurize @11:51:06.83 CS.seek 0ml @11:51:08.77 delay 2:00 @11:52:07.46 delay 3 seconds @11:52:10.49 ATV.dial :ASV @11:52:11.83 ASV.dial :wasteR @11:52:11.92 AS.seek 0.09ml @11:53:08.80 CBV.dial :waste2b @11:53:09.18 CS.reconfigure CSconfig @11:53:09.23 CS.seek :empty @11:53:14.54 delay 1:00 @11:53:52.74 delay 30 seconds @11:54:14.57 CTV.dial :air,avoiding: :intake @11:54:16.06 Storing wcr puck in Collection Clamp to tube 2 @11:54:16.25 SC.reconfigure SCtight @11:54:16.30 SC.seek 2 @11:54:18.50 SC.reconfigure SCloose @11:54:18.74 Elbow.reconfigure TightElbow @11:54:18.79 Elbow.seek 2 @11:54:20.71 Forearm.reconfigure TightForearm @11:54:20.76 Forearm.seek 2 + 700 counts @11:54:21.78 SE.reconfigure SEpick @11:54:21.86 SE.seek beamBreak @11:54:22.76 ATV.dial :extSrc @11:54:23.51 delay 3 seconds @11:54:24.29 SE.configure SEconfig @11:54:24.46 Elbow.reconfigure LimpElbow @11:54:24.52 Forearm.reconfigure LooseForearm @11:54:24.57 Forearm.seek 2 @11:54:24.87 Elbow.hold @11:54:24.95 Elbow.reconfigure TightElbow @11:54:25.06 SE.seek 7.07 Pucks below Top Plate @11:54:25.56 Forearm.force -20 @11:54:25.61 Forearm.seek 2 + 332 counts @11:54:25.95 Forearm.reconfigure TightForearm @11:54:26.00 Forearm.seek 2 + 700 counts @11:54:26.32 SE.seek 8.07 Pucks below Top Plate @11:54:26.53 ATV.dial :ElutionWater @11:54:26.95 Forearm.reconfigure LooseForearm @11:54:27.00 Forearm.seek :retract @11:54:27.61 AS.seek 0.12ml @11:54:27.95 Elbow.seek CC @11:54:29.69 Elbow.reconfigure LooseElbow @11:54:29.80 Forearm.seek CC @11:54:30.86 Hand.close @11:54:32.16 CC.seek :open @11:54:33.93 ATV.dial :air3 @11:54:34.53 AS.seek 0.13ml @11:54:36.68 Forearm.seek :retract @11:54:36.80 delay 2 seconds @11:54:37.73 Elbow.reconfigure TightElbow @11:54:37.78 Elbow.seek 2 @11:54:38.82 ATV.dial :bleach @11:54:39.30 AS.seek 0.23ml @11:54:39.74 Elbow.reconfigure LimpElbow @11:54:39.80 Forearm.seek 2 @11:54:40.81 Elbow.hold @11:54:40.89 Elbow.reconfigure TightElbow @11:54:40.94 Hand.open @11:54:41.66 Forearm.force -20 @11:54:41.71 Forearm.seek 2 + 347 counts @11:54:42.07 Forearm.reconfigure TightForearm @11:54:42.12 Forearm.seek 2 + 700 counts @11:54:42.54 SE.seek :down @11:54:43.68 Forearm.reconfigure LooseForearm @11:54:43.73 Forearm.seek :retract @11:54:44.51 SE.coast @11:54:44.68 SC.coast @11:54:44.72 Starting finalPurge @11:54:44.74 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:54:44.82 Hand.open @11:54:45.16 Elbow.seek FlushPuck::Garage @11:54:47.20 Elbow.reconfigure LooseElbow @11:54:47.30 Forearm.seek :garage @11:54:48.31 Hand.close @11:54:49.03 Forearm.seek :retract @11:54:50.02 Elbow.reconfigure TightElbow @11:54:50.07 Elbow.seek CC @11:54:52.94 Elbow.reconfigure LooseElbow @11:54:53.05 Forearm.reconfigure TightForearm @11:54:53.10 Forearm.seek CC @11:54:54.43 CC.seek :closed @11:54:59.35 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.00 @11:54:59.45 Hand.open @11:54:59.76 delay 3 seconds @11:55:00.10
Forearm.reconfigure LooseForearm @11:55:00.15 Forearm.seek :retract @11:55:01.13 Flushing the Collection Valve::Manifold @11:55:01.27 CSV.dial CSR @11:55:01.83 Pulling 5ml of CSR.flush @11:55:01.84 CSR.select :flush @11:55:01.96 CS.seek 5ml @11:55:02.78 ATV.dial :air3 @11:55:03.33 delay 3 seconds @11:55:06.42 AS.seek 0.255ml @11:55:11.73 delay 3 seconds @11:55:14.76 ATV.dial :bleach @11:55:15.37 AS.seek 0.24ml @11:55:15.82
delay 5 seconds @11:55:18.63 delay 3 seconds @11:55:20.85
CSR.select :air @11:55:21.02 CS.reconfigure CSfast @11:55:21.06 CS.seek 7.5ml @11:55:21.65 ATV.dial :ASV @11:55:22.65 ASV.dial :SPEdelivery @11:55:23.02 AFV.select :carrierSolution @11:55:23.13 delay 2 seconds @11:55:25.16 AS.reconfigure ASconfig @11:55:25.21 AS.seek 0.5ml @11:55:27.91
CSV.dial :waste @11:55:29.29 CS.reconfigure CSconfig @11:55:29.35 CS.seek 0ml @11:55:40.44 delay 1 second @11:55:41.46 AFV.select :holdingCoil @11:55:41.61 AS.reconfigure ASslow1 @11:55:41.67 AS.seek 0.17ml @11:55:49.45
Flushing lower manifold's waste1 port @11:55:49.59 CSV.dial :flush @11:55:50.08 CS.reconfigure CSfast @11:55:50.12 CS.seek 5ml @11:56:02.82 delay 5 seconds @11:56:07.85 CSV.dial :air @11:56:08.39 CS.seek 7ml @11:56:14.07 CSV.dial CTV @11:56:14.10
CTV.dial CSV @11:56:15.59 CBV.dial :waste1 @11:56:16.44 CS.seek 0ml @11:56:33.63 Flushing lower manifold's waste2b port @11:56:33.77 CSV.dial :flush @11:56:34.58 CS.seek 5ml @11:56:47.17 delay 5 seconds @11:56:48.33 SPE.hold 2:46:40,95C @11:56:48.38 delay 3:00 @11:56:52.20
CSV.dial :air @11:56:52.77 CS.seek 7ml @11:56:58.63 CSV.dial CTV @11:56:58.66
CTV.dial CSV @11:56:59.15 CBV.dial :waste2b @11:57:00.02 CS.seek 0ml @11:57:17.92 Flushing upper manifold @11:57:18.06 CSV.dial :flush @11:57:18.88 CS.seek 5ml @11:57:31.95 delay 5 seconds @11:57:36.98 CSV.dial :air @11:57:37.55 CS.seek 7ml @11:57:43.45 CSV.dial CBV @11:57:43.48
CBV.dial CSV @11:57:44.68 CTV.dial :air @11:57:46.16 CS.seek 0ml @11:58:04.23 delay 7 seconds @11:58:11.27 CS.seek 3ml @11:58:19.51 CSV.dial :waste @11:58:20.00 CS.seek 0ml @11:58:29.23 CSV.dial :flush @11:58:29.74 CS.seek 3ml @11:58:38.01 delay 5 seconds @11:58:43.04 CSV.dial :air @11:58:43.61 CS.seek 5ml @11:58:49.51 CSV.dial :air @11:58:49.53 CS.seek 0ml @11:59:02.60 delay 7 seconds @11:59:09.64 CS.seek 3ml @11:59:17.92 CSV.dial :waste @11:59:18.76 CS.seek 0ml @11:59:27.04 Disinfecting external Intake with mfbkill @11:59:27.18 CSV.dial CSR @11:59:28.69 Pulling 8ml of CSR.mfbkill @11:59:28.70 CSR.select :mfbkill @11:59:28.82 CS.seek 8ml @11:59:48.44 AFV.select :carrierSolution @11:59:48.54 delay 2 seconds @11:59:49.26
delay 5 seconds @11:59:50.57 AS.reconfigure ASconfig @11:59:50.62 AS.seek 1ml @11:59:54.28
CSR.select :air @11:59:54.41 CS.seek 10ml @12:00:00.33 CSV.dial CBV @12:00:00.36
CBV.dial CSV @12:00:01.51 CTV.dial :intake @12:00:02.34 Intake.select :open @12:00:02.46 CS.seek 2ml @12:00:23.86 Intake.select :close @12:00:23.97 CTV.dial :air @12:00:24.81 CS.reconfigure CSconfig @12:00:24.86 CS.seek 0ml @12:00:30.98 Flushing lower manifold's waste1 port @12:00:30.99 CSV.dial CSR @12:00:32.19 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @12:00:32.32 CS.seek 4ml @12:00:38.60 delay 1 second @12:00:39.62 AFV.select :holdingCoil @12:00:39.72 delay 1 second @12:00:40.74 ASV.dial :wasteD @12:00:41.75 AS.seek 0ml @12:00:43.58
delay 5 seconds @12:00:48.62 CSR.select :air @12:00:48.75 CS.reconfigure CSfast @12:00:48.79 CS.seek 10ml @12:01:04.23 CSV.dial CTV @12:01:04.26
CTV.dial CSV @12:01:05.83 CBV.dial :waste1 @12:01:06.35 CS.seek 0ml @12:01:31.45 Flushing lower manifold's waste2b port @12:01:31.46 CSV.dial CSR @12:01:32.02 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @12:01:32.15 CS.reconfigure CSconfig @12:01:32.20 CS.seek 4ml @12:01:39.53 Priming Eluate @12:01:39.54 AFV.select :carrierSolution @12:01:39.65 delay 2 seconds @12:01:41.69 AS.seek 0.5ml @12:01:43.33
delay 5 seconds @12:01:48.36 CSR.select :air @12:01:48.48 CS.reconfigure CSfast @12:01:48.52 CS.seek 10ml @12:02:03.83 CSV.dial CTV @12:02:03.87
CTV.dial CSV @12:02:04.37 CBV.dial :waste2b @12:02:05.21 CS.seek 0ml @12:02:10.71 delay 1 second @12:02:11.73 AFV.select :holdingCoil @12:02:11.83 ATV.dial ASV @12:02:11.84 ASV.dial :SPErecovery @12:02:12.43 Swish #1 of 2 @12:02:12.51 AS.reconfigure ASslow0 @12:02:12.56 AS.seek 0.6ml @12:02:23.00 AS.seek 0.5ml @12:02:29.92
Killing upper manifold @12:02:29.93 CSV.dial CSR @12:02:30.48 Pulling 4ml of CSR.mfbkill @12:02:30.49 CSR.select :mfbkill @12:02:30.62 CS.reconfigure CSconfig @12:02:30.67 CS.seek 4ml @12:02:33.36 Swish #2 of 2 @12:02:33.45 AS.seek 0.6ml @12:02:42.52
delay 5 seconds @12:02:43.99 AS.seek 0.5ml @12:02:47.54
CSR.select :air @12:02:47.67 CS.reconfigure CSfast @12:02:47.71 CS.seek 10ml @12:02:54.37 delay 3 seconds @12:02:57.46 AS.reconfigure ASslow1 @12:02:57.52 AS.seek 0.684ml @12:03:02.74 CSV.dial CBV @12:03:02.77
CBV.dial CSV @12:03:03.94 CTV.dial :air @12:03:05.38 CS.seek 0ml @12:03:29.80 delay 7 seconds @12:03:34.78 delay 3 seconds @12:03:36.83
CS.seek 3ml @12:03:37.81 Eluate primed @12:03:37.82 ATV.dial :waste2 @12:03:38.42 AS.reconfigure ASnorm @12:03:38.47 AS.seek 0ml @12:03:45.05
CSV.dial :waste @12:03:45.54 CS.seek 0ml @12:03:53.61 Flushing upper manifold @12:03:53.75 CSV.dial :flush @12:03:54.25 CS.seek 5ml @12:04:07.14 delay 5 seconds @12:04:12.17 CSV.dial :air @12:04:12.86 CS.seek 7.001ml @12:04:13.27 SPE.coast @12:04:13.36 ASV.dial :SPErecovery @12:04:13.38 Setting up PCR for OM -- writing /var/log/esp/OM20may2212h300ml.pcr @12:04:13.40 Building PCR Slug from ASV with MM3 mix, PP5 primer @12:04:13.42 Priming with carrier @12:04:13.43 ATV.dial ARV @12:04:14.36 ARV.dial :carrier @12:04:14.71 AS.seek 0.5ml @12:04:18.49 CSV.dial CBV @12:04:18.52
CBV.dial CSV @12:04:19.72 CTV.dial :air @12:04:19.74 CS.seek 0ml @12:04:36.83 delay 7 seconds @12:04:40.25 AFV.select :carrierSolution @12:04:40.36 delay 2 seconds @12:04:42.40 AS.reconfigure ASconfig @12:04:42.45 AS.seek 1ml @12:04:43.86
CS.seek 3ml @12:04:51.74 CSV.dial :waste @12:04:52.22 CS.seek 0ml @12:05:00.27 CSV.dial :flush @12:05:00.75 CS.seek 3ml @12:05:08.86 delay 5 seconds @12:05:11.50 delay 1 second @12:05:12.52 AFV.select :holdingCoil @12:05:12.62 delay 1 second @12:05:13.65 ATV.dial :waste @12:05:13.88
CSV.dial :air @12:05:14.43 CS.seek 5ml @12:05:15.06 AS.reconfigure ASnorm @12:05:15.12 AS.seek :empty @12:05:20.13
CSV.dial :air @12:05:20.17 CS.seek 0ml @12:05:32.71 delay 7 seconds @12:05:39.75 CS.seek 3ml @12:05:47.64 CSV.dial :waste @12:05:48.42 CS.seek 0ml @12:05:56.33 Flushing lower manifold's waste1 port @12:05:56.47 CSV.dial :flush @12:05:56.97 CS.seek 5ml @12:06:05.87 AFV.select :carrierSolution @12:06:05.98 delay 2 seconds @12:06:08.01 AS.reconfigure ASconfig @12:06:08.06 AS.seek 0.5ml @12:06:09.43
delay 5 seconds @12:06:14.45 CSV.dial :air @12:06:14.98 CS.seek 7ml @12:06:20.55 CSV.dial CTV @12:06:21.22
CTV.dial CSV @12:06:22.77 CBV.dial :waste1 @12:06:23.29 CS.seek 0ml @12:06:37.14 delay 1 second @12:06:38.17 AFV.select :holdingCoil @12:06:38.27 Building Slug @12:06:38.28 ATV.dial :ARV @12:06:39.93 delay 2 seconds @12:06:40.36
Flushing lower manifold's waste2b port @12:06:40.50 CSV.dial :flush @12:06:41.31 CS.seek 5ml @12:06:41.96 ARV.dial :air1 @12:06:42.35 delay 2 seconds @12:06:44.44 AS.reconfigure ASslow2 @12:06:44.49 AS.seek 0.506ml @12:06:50.64 delay 2 seconds @12:06:52.67 ARV.dial :MM3 @12:06:53.67 delay 2 seconds @12:06:53.77
delay 5 seconds @12:06:55.70 Adding MM3 @12:06:55.79 AS.seek 0.524ml @12:06:58.79
CSV.dial :air @12:06:59.37 CS.seek 7ml @12:07:05.08 CSV.dial CTV @12:07:05.11
CTV.dial CSV @12:07:05.58 CBV.dial :waste2b @12:07:06.42 CS.seek 0ml @12:07:14.09 delay 25 seconds @12:07:23.49
Disinfecting external Intake with kill @12:07:23.63 CSV.dial CSR @12:07:24.18 Pulling 8ml of CSR.kill @12:07:24.19 CSR.select :kill @12:07:24.31 CS.seek 8ml @12:07:39.12 ARV.dial :PP5 @12:07:39.50 Adding PP5 @12:07:39.52 delay 2 seconds @12:07:41.60 AS.seek 0.53ml @12:07:43.77
delay 5 seconds @12:07:47.98 delay 10 seconds @12:07:48.79
CSR.select :air @12:07:48.91 CS.seek 10ml @12:07:54.62 CSV.dial CBV @12:07:54.65
CBV.dial CSV @12:07:55.79 CTV.dial :intake @12:07:56.58 Intake.select :open @12:07:56.70 CS.seek 2ml @12:07:58.00 ARV.dial :air3 @12:07:58.39 adding ARV air @12:07:58.41 delay 2 seconds @12:08:00.49 AS.seek 0.536ml @12:08:06.73 delay 5 seconds @12:08:11.76 ARV.dial :carrier @12:08:12.98 delay 7 seconds @12:08:17.37
Intake.select :close @12:08:17.49 CTV.dial :air @12:08:18.30 CS.reconfigure CSconfig @12:08:18.35 CS.seek 0ml @12:08:20.07 AS.seek 0.594ml @12:08:24.43
CSV.dial CSR @12:08:25.58 Pulling 5ml of CSR.flush @12:08:25.59 CSR.select :flush @12:08:25.72 CS.seek 5ml @12:08:39.55 delay 5 seconds @12:08:44.58 CSR.select :air @12:08:44.71 CS.seek 10ml @12:08:58.52 CSV.dial CBV @12:08:59.61 CS.seek 0ml @12:09:18.50 delay 5 seconds @12:09:23.53 ATV.dial :ASV @12:09:23.92 delay 2 seconds @12:09:26.01 AS.seek 0.6ml @12:09:26.30
Garaging FlushPuck in Collection Clamp @12:09:26.38 Elbow.reconfigure TightElbow @12:09:26.43 Elbow.seek CC @12:09:26.67 Elbow.reconfigure LooseElbow @12:09:27.44 Forearm.seek CC @12:09:28.41 Hand.close @12:09:29.06 CC.seek :open @12:09:32.26 delay 4 seconds @12:09:33.44
Forearm.seek :retract @12:09:34.46 Elbow.reconfigure TightElbow @12:09:34.51 Elbow.seek :garage @12:09:36.29 ATV.dial ARV @12:09:36.81 delay 2 seconds @12:09:37.81
Elbow.reconfigure LooseElbow @12:09:37.91 Forearm.reconfigure TightForearm @12:09:37.96 Forearm.seek :garage @12:09:38.84 ARV.dial :carrier @12:09:38.86 delay 2 seconds @12:09:39.11
Hand.open @12:09:39.75 Forearm.reconfigure LooseForearm @12:09:39.80 Forearm.seek :retract @12:09:40.81 Starting initialPurge @12:09:40.82 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:09:40.90 Hand.open @12:09:41.02 AS.seek 0.65ml @12:09:41.24
Elbow.reconfigure TightElbow @12:09:41.29 Elbow.seek FlushPuck::Garage @12:09:41.49 Elbow.reconfigure LooseElbow @12:09:41.60 Forearm.seek :garage @12:09:42.61 Hand.close @12:09:43.31 Forearm.seek :retract @12:09:44.32 Elbow.reconfigure TightElbow @12:09:44.37 Elbow.seek CC @12:09:47.34 Elbow.reconfigure LooseElbow @12:09:47.45 Forearm.reconfigure TightForearm @12:09:47.50 Forearm.seek CC @12:09:48.83 CC.seek :closed @12:09:53.07 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.98 @12:09:53.16 Hand.open @12:09:53.87 Forearm.reconfigure LooseForearm @12:09:53.91 Forearm.seek :retract @12:09:54.94 Flushing the Collection Valve::Manifold @12:09:55.08 CSV.dial CSR @12:09:56.22 Pulling 4.999ml of CSR.flush @12:09:56.23 CSR.select :flush @12:09:56.36 CS.seek 5ml @12:10:10.15 delay 5 seconds @12:10:15.18 CSR.select :air @12:10:15.36 CS.reconfigure CSfast @12:10:15.40 CS.seek 7.5ml @12:10:22.21 CSV.dial :waste @12:10:23.57 CS.reconfigure CSconfig @12:10:23.62 CS.seek 0ml @12:10:31.55 delay 4 seconds @12:10:35.58 ATV.dial PCR @12:10:36.17 ASV.dial :waste1 @12:10:36.95 ARV.dial :air1 @12:10:37.34 Positioning Slug by 0.324ml @12:10:37.43 AS.seek 0.326ml @12:10:43.96
Purging external Intake @12:10:44.81 CSV.dial :flush @12:10:45.29 CS.reconfigure CSfast @12:10:45.34 CS.seek 10ml @12:11:09.18 delay 5 seconds @12:11:14.21 CSV.dial CBV @12:11:14.25
CBV.dial CSV @12:11:15.06 CTV.dial :intake @12:11:15.87 Intake.select :open @12:11:15.99 CS.seek 0ml @12:11:41.31 Intake.select :close @12:11:41.42 CTV.dial :air @12:11:42.23 Flushing upper manifold @12:11:42.37 CSV.dial :flush @12:11:43.16 CS.seek 5ml @12:11:55.62 delay 5 seconds @12:12:00.65 CSV.dial :air @12:12:01.20 CS.seek 7ml @12:12:06.81 CSV.dial CBV @12:12:06.84
CBV.dial CSV @12:12:07.96 CTV.dial :air @12:12:07.98 CS.seek 0ml @12:12:24.90 delay 7 seconds @12:12:31.94 CS.seek 3ml @12:12:40.03 CSV.dial :waste @12:12:40.52 CS.seek 0ml @12:12:48.71 CSV.dial :flush @12:12:49.21 CS.seek 3ml @12:12:57.09 delay 5 seconds @12:13:02.12 CSV.dial :air @12:13:02.66 CS.seek 5ml @12:13:08.37 CSV.dial :air @12:13:08.39 CS.seek 0ml @12:13:20.81 delay 7 seconds @12:13:27.85 CS.seek 3ml @12:13:35.75 CSV.dial :waste @12:13:36.55 CS.seek 0ml @12:13:44.47 Garaging FlushPuck in Collection Clamp @12:13:44.55 Elbow.reconfigure TightElbow @12:13:44.60 Elbow.seek CC @12:13:44.83 Elbow.reconfigure LooseElbow @12:13:44.94 Forearm.seek CC @12:13:45.93 Hand.close @12:13:46.56 CC.seek :open @12:13:50.94 Forearm.seek :retract @12:13:51.95 Elbow.reconfigure TightElbow @12:13:52.00 Elbow.seek :garage @12:13:55.18 Elbow.reconfigure LooseElbow @12:13:55.29 Forearm.reconfigure TightForearm @12:13:55.33 Forearm.seek :garage @12:13:56.48 Hand.open @12:13:57.13 Forearm.reconfigure LooseForearm @12:13:57.18 Forearm.seek :retract @12:13:58.17 Completed [stdphase] begun 10:00:51 @12:13:58.18 Preparing to sleep until Sat May 23 10:00:51 +0200 2020 @12:13:58.25 deferred until MFBPCR finish @12:13:58.26
Waiting for MFBPCR til 10:00CEST23-May-20 @12:13:58.28 Continuing at 10:00CEST23-May-20 @12:13:58.34 GoodNews.email "Then, in tube 3: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:150\n{1 => 22, 2 => 9, 3 => 13}\nCan@12:13:58, 10.7C, 47% humidity, 14.4psia, 13.318V, 0.493A, 0.493A avg, 6.57W",Subject: "Waiting until 10:00CEST23-May-20" @12:16:03.55 delay 5 seconds @12:16:08.58 ATV.dial ARV @12:16:09.31 ASV.dial :PCRlock @12:16:09.69 delay 5 seconds @12:16:14.72 Running OM Profile60C350 thermocycling @12:16:14.73 PCR.program Profile60C350 @12:16:14.85 Profile60C350 should complete in < 2:02:45 @12:16:14.86 PCR.run 2:38:26.25 @13:52:47.90 PCR.off @13:52:49.16 PCR for OM completed -- closed /var/log/esp/OM20may2212h300ml.pcr @13:52:49.23 Cleaning PCR @13:52:49.24 AFV.select :carrierSolution @13:52:49.35 delay 2 seconds @13:52:51.38 AS.reconfigure ASconfig @13:52:51.43 AS.seek 0.5ml @13:53:01.74 delay 1 second @13:53:02.76 AFV.select :holdingCoil @13:53:02.86 ASV.dial :waste1 @13:53:03.24 ATV.dial :air3 @13:53:04.47 AS.reconfigure ASslow1 @13:53:04.52 AS.seek 0.51ml @13:53:06.83 delay 2 seconds @13:53:08.85 ATV.dial :bleach @13:53:09.45 AS.seek 0.81ml @13:54:10.05 delay 3 seconds @13:54:13.08 ATV.dial :air3 @13:54:13.62 delay 3 seconds @13:54:16.71 AS.seek 0.835ml @13:54:22.01 delay 3 seconds @13:54:25.04 ATV.dial :bleach @13:54:26.36 AS.seek 0.82ml @13:54:29.65 delay 3 seconds @13:54:32.68 ARV.dial :waste @13:54:33.27 delay 3 seconds @13:54:36.30 ATV.dial :ARV @13:54:37.15 AS.reconfigure ASconfig @13:54:37.20 AS.seek 0.47ml @13:54:57.62 AFV.select :carrierSolution @13:54:57.73 delay 2 seconds @13:54:59.77 AS.seek 1ml @13:55:30.60 delay 1 second @13:55:31.62 AFV.select :holdingCoil @13:55:31.72 delay 1 second @13:55:32.75 AS.reconfigure ASfast @13:55:32.80 AS.seek 0ml @13:56:00.16 AFV.select :carrierSolution @13:56:00.27 delay 2 seconds @13:56:02.30 AS.reconfigure ASconfig @13:56:02.35 AS.seek 1ml @13:57:00.15 delay 1 second @13:57:01.17 AFV.select :holdingCoil @13:57:01.27 delay 1 second @13:57:02.30 AS.reconfigure ASfast @13:57:02.35 AS.seek 0ml @13:57:29.71 AFV.select :carrierSolution @13:57:29.82 delay 2 seconds @13:57:31.85 AS.reconfigure ASconfig @13:57:31.90 AS.seek 1ml @13:58:29.66 delay 1 second @13:58:30.68 AFV.select :holdingCoil @13:58:30.78 delay 1 second @13:58:31.81 AS.reconfigure ASfast @13:58:31.86 AS.seek 0ml @13:58:59.21 ARV.dial between carrier and air1 @13:58:59.70 ATV.dial :air3 @13:59:00.92 AS.reconfigure ASslow1 @13:59:00.97 AS.seek 0.01ml @13:59:03.24 delay 2 seconds @13:59:05.26 ATV.dial :bleach @13:59:05.86 AS.seek 0.81ml @14:01:47.01 delay 3 seconds @14:01:50.04 ATV.dial :air3 @14:01:50.57 delay 3 seconds @14:01:53.66 AS.seek 0.835ml @14:01:58.96 delay 3 seconds @14:02:01.99 ATV.dial :bleach @14:02:02.60 AS.seek 0.82ml @14:02:05.90 delay 3 seconds @14:02:08.93 ATV.dial PCR @14:02:09.31 AS.reconfigure ASconfig @14:02:09.36 AS.seek :empty @14:02:56.81 PCR.program :bleachProfile @14:02:56.87 PCR program should complete in < 2:45 @14:02:56.88 PCR.run 10:00 @14:06:07.78 PCR.off @14:06:07.82 AFV.select :carrierSolution @14:06:07.93 delay 2 seconds @14:06:09.97 AS.seek 1ml @14:07:07.79 delay 1 second @14:07:08.81 AFV.select :holdingCoil @14:07:08.91 delay 1 second @14:07:09.94 AS.reconfigure ASfast @14:07:09.99 AS.seek 0ml @14:07:37.34 delay 3 seconds @14:07:40.37 AFV.select :carrierSolution @14:07:40.47 delay 2 seconds @14:07:42.50 AS.reconfigure ASconfig @14:07:42.55 AS.seek 1ml @14:08:40.34 delay 1 second @14:08:41.36 AFV.select :holdingCoil @14:08:41.46 delay 1 second @14:08:42.49 AS.reconfigure ASfast @14:08:42.55 AS.seek 0ml @14:09:09.89 delay 3 seconds @14:09:12.92 AFV.select :carrierSolution @14:09:13.02 delay 2 seconds @14:09:15.05 AS.reconfigure ASconfig @14:09:15.11 AS.seek 1ml @14:10:12.87 delay 1 second @14:10:13.89 AFV.select :holdingCoil @14:10:13.99 delay 1 second @14:10:15.02 AS.reconfigure ASfast @14:10:15.07 AS.seek 0ml @14:10:42.41 delay 3 seconds @14:10:45.44 AFV.select :carrierSolution @14:10:45.54 delay 2 seconds @14:10:47.57 AS.reconfigure ASconfig @14:10:47.63 AS.seek 1ml @14:11:45.41 delay 1 second @14:11:46.43 AFV.select :holdingCoil @14:11:46.53 delay 1 second @14:11:47.56 AS.reconfigure ASfast @14:11:47.61 AS.seek 0ml @14:12:14.95 delay 3 seconds @14:12:17.98 AFV.select :carrierSolution @14:12:18.08 delay 2 seconds @14:12:20.11 AS.reconfigure ASconfig @14:12:20.87 AS.seek 1ml @14:13:18.63 delay 1 second @14:13:19.65 AFV.select :holdingCoil @14:13:19.75 delay 1 second @14:13:20.78 AS.reconfigure ASnorm @14:13:20.83 AS.seek 0ml @14:14:11.57 Setting up PCR for ML -- writing /var/log/esp/ML20may2214h300ml.pcr @14:14:11.60 Building PCR Slug from ASV with MM2 mix, PP4 primer @14:14:11.61 Priming with carrier @14:14:11.62 ATV.dial ARV @14:14:12.20 ARV.dial :carrier @14:14:12.54 AS.seek 0.5ml @14:14:38.05 AFV.select :carrierSolution @14:14:38.16 delay 2 seconds @14:14:40.19 AS.reconfigure ASconfig @14:14:40.24 AS.seek 1ml @14:15:09.25 delay 1 second @14:15:10.27 AFV.select :holdingCoil @14:15:10.37 delay 1 second @14:15:11.40 ATV.dial :waste @14:15:12.95 AS.reconfigure ASnorm @14:15:13.01 AS.seek :empty @14:16:03.74 AFV.select :carrierSolution @14:16:03.85 delay 2 seconds @14:16:05.88 AS.reconfigure ASconfig @14:16:05.93 AS.seek 0.5ml @14:16:35.00 delay 1 second @14:16:36.02 AFV.select :holdingCoil @14:16:36.12 Building Slug @14:16:36.13 ATV.dial :ARV @14:16:37.67 delay 2 seconds @14:16:39.70 ARV.dial :air1 @14:16:40.09 delay 2 seconds @14:16:42.18 AS.reconfigure ASslow2 @14:16:42.23 AS.seek 0.506ml @14:16:48.46 delay 2 seconds @14:16:50.48 ARV.dial :MM2 @14:16:51.88 delay 2 seconds @14:16:53.91 Adding MM2 @14:16:54.00 AS.seek 0.524ml @14:17:12.47 delay 25 seconds @14:17:37.50 ARV.dial :PP4 @14:17:37.88 Adding PP4 @14:17:37.90 delay 2 seconds @14:17:39.98 AS.seek 0.53ml @14:17:46.49 delay 10 seconds @14:17:56.52 ARV.dial :air3 @14:17:56.91 adding ARV air @14:17:56.93 delay 2 seconds @14:17:59.01 AS.seek 0.536ml @14:18:05.41 delay 5 seconds @14:18:10.44 ARV.dial :carrier @14:18:11.63 delay 7 seconds @14:18:18.72 AS.seek 0.594ml @14:19:17.28 delay 5 seconds @14:19:22.31 ATV.dial :ASV @14:19:22.71 delay 2 seconds @14:19:24.80 AS.seek 0.6ml @14:19:30.99 delay 4 seconds @14:19:35.02 ATV.dial ARV @14:19:35.64 delay 2 seconds @14:19:37.67 ARV.dial :carrier @14:19:37.68 delay 2 seconds @14:19:39.77 AS.seek 0.65ml @14:20:30.21 delay 4 seconds @14:20:34.24 ATV.dial PCR @14:20:34.82 ASV.dial :waste1 @14:20:34.83 ARV.dial :air1 @14:20:35.23 Positioning Slug by 0.324ml @14:20:35.32 AS.seek 0.326ml @14:26:01.38 delay 5 seconds @14:26:06.41 ATV.dial ARV @14:26:07.13 ASV.dial :PCRlock @14:26:07.52 delay 5 seconds @14:26:12.55 Running ML Profile60C350 thermocycling @14:26:12.57 PCR.program Profile60C350 @14:26:12.67 Profile60C350 should complete in < 2:02:45 @14:26:12.69 PCR.run 2:38:26.25 @16:02:21.22 PCR.off @16:02:22.49 PCR for ML completed -- closed /var/log/esp/ML20may2214h300ml.pcr @16:02:22.56 Cleaning PCR @16:02:22.57 AFV.select :carrierSolution @16:02:22.68 delay 2 seconds @16:02:24.71 AS.reconfigure ASconfig @16:02:24.76 AS.seek 0.5ml @16:02:35.08 delay 1 second @16:02:36.10 AFV.select :holdingCoil @16:02:36.20 ASV.dial :waste1 @16:02:36.58 ATV.dial :air3 @16:02:37.80 AS.reconfigure ASslow1 @16:02:37.85 AS.seek 0.51ml @16:02:40.12 delay 2 seconds @16:02:42.14 ATV.dial :bleach @16:02:42.74 AS.seek 0.81ml @16:03:43.37 delay 3 seconds @16:03:46.40 ATV.dial :air3 @16:03:46.94 delay 3 seconds @16:03:50.03 AS.seek 0.835ml @16:03:55.31 delay 3 seconds @16:03:58.34 ATV.dial :bleach @16:03:58.94 AS.seek 0.82ml @16:04:02.25 delay 3 seconds @16:04:05.28 ARV.dial :waste @16:04:05.86 delay 3 seconds @16:04:08.89 ATV.dial :ARV @16:04:09.73 AS.reconfigure ASconfig @16:04:09.79 AS.seek 0.47ml @16:04:30.20 AFV.select :carrierSolution @16:04:30.31 delay 2 seconds @16:04:32.35 AS.seek 1ml @16:05:03.09 delay 1 second @16:05:04.11 AFV.select :holdingCoil @16:05:04.21 delay 1 second @16:05:05.24 AS.reconfigure ASfast @16:05:05.29 AS.seek 0ml @16:05:32.63 AFV.select :carrierSolution @16:05:32.75 delay 2 seconds @16:05:34.78 AS.reconfigure ASconfig @16:05:34.83 AS.seek 1ml @16:06:32.61 delay 1 second @16:06:33.63 AFV.select :holdingCoil @16:06:33.73 delay 1 second @16:06:34.76 AS.reconfigure ASfast @16:06:34.81 AS.seek 0ml @16:07:02.16 AFV.select :carrierSolution @16:07:02.27 delay 2 seconds @16:07:04.30 AS.reconfigure ASconfig @16:07:04.35 AS.seek 1ml @16:08:02.13 delay 1 second @16:08:03.15 AFV.select :holdingCoil @16:08:03.25 delay 1 second @16:08:04.28 AS.reconfigure ASfast @16:08:04.33 AS.seek 0ml @16:08:31.69 ARV.dial between carrier and air1 @16:08:32.88 ATV.dial :air3 @16:08:34.10 AS.reconfigure ASslow1 @16:08:34.15 AS.seek 0.01ml @16:08:36.44 delay 2 seconds @16:08:38.46 ATV.dial :bleach @16:08:39.06 AS.seek 0.81ml @16:11:20.19 delay 3 seconds @16:11:23.22 ATV.dial :air3 @16:11:23.74 delay 3 seconds @16:11:26.83 AS.seek 0.835ml @16:11:32.23 delay 3 seconds @16:11:35.26 ATV.dial :bleach @16:11:35.86 AS.seek 0.82ml @16:11:39.10 delay 3 seconds @16:11:42.13 ATV.dial PCR @16:11:42.52 AS.reconfigure ASconfig @16:11:42.57 AS.seek :empty @16:12:30.02 PCR.program :bleachProfile @16:12:30.08 PCR program should complete in < 2:45 @16:12:30.09 PCR.run 10:00 @16:15:44.02 PCR.off @16:15:44.06 AFV.select :carrierSolution @16:15:44.17 delay 2 seconds @16:15:46.21 AS.seek 1ml @16:16:44.04 delay 1 second @16:16:45.06 AFV.select :holdingCoil @16:16:45.16 delay 1 second @16:16:46.20 AS.reconfigure ASfast @16:16:46.26 AS.seek 0ml @16:17:13.58 delay 3 seconds @16:17:16.61 AFV.select :carrierSolution @16:17:16.71 delay 2 seconds @16:17:18.74 AS.reconfigure ASconfig @16:17:18.80 AS.seek 1ml @16:18:16.59 delay 1 second @16:18:17.61 AFV.select :holdingCoil @16:18:17.71 delay 1 second @16:18:18.74 AS.reconfigure ASfast @16:18:18.79 AS.seek 0ml @16:18:46.12 delay 3 seconds @16:18:49.15 AFV.select :carrierSolution @16:18:49.25 delay 2 seconds @16:18:51.28 AS.reconfigure ASconfig @16:18:51.34 AS.seek 1ml @16:19:49.12 delay 1 second @16:19:50.14 AFV.select :holdingCoil @16:19:50.24 delay 1 second @16:19:51.27 AS.reconfigure ASfast @16:19:51.32 AS.seek 0ml @16:20:18.67 delay 3 seconds @16:20:21.70 AFV.select :carrierSolution @16:20:21.80 delay 2 seconds @16:20:23.83 AS.reconfigure ASconfig @16:20:23.88 AS.seek 1ml @16:21:21.71 delay 1 second @16:21:22.73 AFV.select :holdingCoil @16:21:22.83 delay 1 second @16:21:23.86 AS.reconfigure ASfast @16:21:23.91 AS.seek 0ml @16:21:51.23 delay 3 seconds @16:21:54.26 AFV.select :carrierSolution @16:21:54.36 delay 2 seconds @16:21:56.40 AS.reconfigure ASconfig @16:21:56.45 AS.seek 1ml @16:22:54.24 delay 1 second @16:22:55.26 AFV.select :holdingCoil @16:22:55.36 delay 1 second @16:22:56.39 AS.reconfigure ASnorm @16:22:56.44 AS.seek 0ml @16:23:47.14 Setting up PCR for CG -- writing /var/log/esp/CG20may2216h300ml.pcr @16:23:47.16 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:23:47.17 Priming with carrier @16:23:47.18 ATV.dial ARV @16:23:47.78 ARV.dial :carrier @16:23:48.13 AS.seek 0.5ml @16:24:13.68 AFV.select :carrierSolution @16:24:13.79 delay 2 seconds @16:24:15.82 AS.reconfigure ASconfig @16:24:15.87 AS.seek 1ml @16:24:44.90 delay 1 second @16:24:45.92 AFV.select :holdingCoil @16:24:46.02 delay 1 second @16:24:47.05 ATV.dial :waste @16:24:48.62 AS.reconfigure ASnorm @16:24:48.67 AS.seek :empty @16:25:39.42 AFV.select :carrierSolution @16:25:39.53 delay 2 seconds @16:25:41.56 AS.reconfigure ASconfig @16:25:41.61 AS.seek 0.5ml @16:26:10.72 delay 1 second @16:26:11.74 AFV.select :holdingCoil @16:26:11.84 Building Slug @16:26:11.85 ATV.dial :ARV @16:26:13.39 delay 2 seconds @16:26:15.42 ARV.dial :air1 @16:26:16.51 delay 2 seconds @16:26:18.60 AS.reconfigure ASslow2 @16:26:18.66 AS.seek 0.506ml @16:26:24.86 delay 2 seconds @16:26:26.89 ARV.dial :MM1 @16:26:27.47 delay 2 seconds @16:26:29.50 Adding MM1 @16:26:29.57 AS.seek 0.524ml @16:26:48.09 delay 25 seconds @16:27:13.12 ARV.dial :PP2 @16:27:13.50 Adding PP2 @16:27:13.52 delay 2 seconds @16:27:15.60 AS.seek 0.53ml @16:27:21.79 delay 10 seconds @16:27:31.82 ARV.dial :air2 @16:27:32.18 adding ARV air @16:27:32.20 delay 2 seconds @16:27:34.28 AS.seek 0.536ml @16:27:40.51 delay 5 seconds @16:27:45.54 ARV.dial :carrier @16:27:46.75 delay 7 seconds @16:27:53.85 AS.seek 0.594ml @16:28:52.47 delay 5 seconds @16:28:57.50 ATV.dial :ASV @16:28:57.89 delay 2 seconds @16:28:59.98 AS.seek 0.6ml @16:29:06.30 delay 4 seconds @16:29:10.33 ATV.dial ARV @16:29:10.97 delay 2 seconds @16:29:13.00 ARV.dial :carrier @16:29:13.01 delay 2 seconds @16:29:15.10 AS.seek 0.65ml @16:30:05.80 delay 4 seconds @16:30:09.83 ATV.dial PCR @16:30:10.42 ASV.dial :waste1 @16:30:10.43 ARV.dial :air1 @16:30:10.82 Positioning Slug by 0.324ml @16:30:10.91 AS.seek 0.326ml @16:35:36.97 delay 5 seconds @16:35:42.00 ATV.dial ARV @16:35:42.75 ASV.dial :PCRlock @16:35:43.14 delay 5 seconds @16:35:48.17 Running CG Profile60C300 thermocycling @16:35:48.18 PCR.program Profile60C300 @16:35:48.29 Profile60C300 should complete in < 1:59:25 @16:35:48.30 PCR.run 2:34:16.25 @18:11:19.15 PCR.off @18:11:19.83 PCR for CG completed -- closed /var/log/esp/CG20may2216h300ml.pcr @18:11:19.90 Cleaning PCR @18:11:19.91 AFV.select :carrierSolution @18:11:20.02 delay 2 seconds @18:11:22.05 AS.reconfigure ASconfig @18:11:22.10 AS.seek 0.5ml @18:11:32.39 delay 1 second @18:11:33.41 AFV.select :holdingCoil @18:11:33.51 ASV.dial :waste1 @18:11:33.90 ATV.dial :air3 @18:11:35.13 AS.reconfigure ASslow1 @18:11:35.18 AS.seek 0.51ml @18:11:37.48 delay 2 seconds @18:11:39.50 ATV.dial :bleach @18:11:40.11 AS.seek 0.81ml @18:12:40.70 delay 3 seconds @18:12:43.73 ATV.dial :air3 @18:12:44.26 delay 3 seconds @18:12:47.35 AS.seek 0.835ml @18:12:52.64 delay 3 seconds @18:12:55.67 ATV.dial :bleach @18:12:56.27 AS.seek 0.82ml @18:12:59.59 delay 3 seconds @18:13:02.61 ARV.dial :waste @18:13:03.20 delay 3 seconds @18:13:06.23 ATV.dial :ARV @18:13:07.08 AS.reconfigure ASconfig @18:13:07.13 AS.seek 0.47ml @18:13:27.53 AFV.select :carrierSolution @18:13:27.64 delay 2 seconds @18:13:29.68 AS.seek 1ml @18:14:00.43 delay 1 second @18:14:01.45 AFV.select :holdingCoil @18:14:01.55 delay 1 second @18:14:02.58 AS.reconfigure ASfast @18:14:02.63 AS.seek 0ml @18:14:29.95 AFV.select :carrierSolution @18:14:30.06 delay 2 seconds @18:14:32.09 AS.reconfigure ASconfig @18:14:32.14 AS.seek 1ml @18:15:29.90 delay 1 second @18:15:30.92 AFV.select :holdingCoil @18:15:31.02 delay 1 second @18:15:32.05 AS.reconfigure ASfast @18:15:32.10 AS.seek 0ml @18:15:59.42 AFV.select :carrierSolution @18:15:59.53 delay 2 seconds @18:16:01.56 AS.reconfigure ASconfig @18:16:01.61 AS.seek 1ml @18:16:59.39 delay 1 second @18:17:00.41 AFV.select :holdingCoil @18:17:00.51 delay 1 second @18:17:01.54 AS.reconfigure ASfast @18:17:01.59 AS.seek 0ml @18:17:28.95 ARV.dial between carrier and air1 @18:17:29.44 ATV.dial :air3 @18:17:30.66 AS.reconfigure ASslow1 @18:17:30.71 AS.seek 0.01ml @18:17:33.00 delay 2 seconds @18:17:35.02 ATV.dial :bleach @18:17:35.63 AS.seek 0.81ml @18:20:16.78 delay 3 seconds @18:20:19.81 ATV.dial :air3 @18:20:20.34 delay 3 seconds @18:20:23.43 AS.seek 0.835ml @18:20:28.70 delay 3 seconds @18:20:31.73 ATV.dial :bleach @18:20:32.34 AS.seek 0.82ml @18:20:35.64 delay 3 seconds @18:20:38.67 ATV.dial PCR @18:20:39.05 AS.reconfigure ASconfig @18:20:39.10 AS.seek :empty @18:21:26.55 PCR.program :bleachProfile @18:21:26.60 PCR program should complete in < 2:45 @18:21:26.61 PCR.run 10:00 @18:24:40.56 PCR.off @18:24:40.58 AFV.select :carrierSolution @18:24:40.69 delay 2 seconds @18:24:43.44 AS.seek 1ml @18:25:41.27 delay 1 second @18:25:42.29 AFV.select :holdingCoil @18:25:42.39 delay 1 second @18:25:43.42 AS.reconfigure ASfast @18:25:43.47 AS.seek 0ml @18:26:10.83 delay 3 seconds @18:26:13.86 AFV.select :carrierSolution @18:26:13.96 delay 2 seconds @18:26:15.99 AS.reconfigure ASconfig @18:26:16.05 AS.seek 1ml @18:27:13.85 delay 1 second @18:27:14.87 AFV.select :holdingCoil @18:27:14.97 delay 1 second @18:27:16.00 AS.reconfigure ASfast @18:27:16.05 AS.seek 0ml @18:27:43.38 delay 3 seconds @18:27:46.41 AFV.select :carrierSolution @18:27:46.51 delay 2 seconds @18:27:48.54 AS.reconfigure ASconfig @18:27:48.59 AS.seek 1ml @18:28:46.40 delay 1 second @18:28:47.42 AFV.select :holdingCoil @18:28:47.52 delay 1 second @18:28:48.55 AS.reconfigure ASfast @18:28:48.60 AS.seek 0ml @18:29:15.96 delay 3 seconds @18:29:18.99 AFV.select :carrierSolution @18:29:19.09 delay 2 seconds @18:29:21.12 AS.reconfigure ASconfig @18:29:21.18 AS.seek 1ml @18:30:18.96 delay 1 second @18:30:19.98 AFV.select :holdingCoil @18:30:20.08 delay 1 second @18:30:21.11 AS.reconfigure ASfast @18:30:21.17 AS.seek 0ml @18:30:48.52 delay 3 seconds @18:30:51.55 AFV.select :carrierSolution @18:30:51.65 delay 2 seconds @18:30:53.68 AS.reconfigure ASconfig @18:30:53.74 AS.seek 1ml @18:31:51.51 delay 1 second @18:31:52.53 AFV.select :holdingCoil @18:31:52.63 delay 1 second @18:31:53.66 AS.reconfigure ASnorm @18:31:53.71 AS.seek 0ml @18:32:44.42 Setting up PCR for NM -- writing /var/log/esp/NM20may2218h300ml.pcr @18:32:44.44 Building PCR Slug from ASV with MM1 mix, PP1 primer @18:32:44.45 Priming with carrier @18:32:44.46 ATV.dial ARV @18:32:45.06 ARV.dial :carrier @18:32:45.41 AS.seek 0.5ml @18:33:10.99 AFV.select :carrierSolution @18:33:11.10 delay 2 seconds @18:33:13.13 AS.reconfigure ASconfig @18:33:13.18 AS.seek 1ml @18:33:42.21 delay 1 second @18:33:43.23 AFV.select :holdingCoil @18:33:43.33 delay 1 second @18:33:44.36 ATV.dial :waste @18:33:45.92 AS.reconfigure ASnorm @18:33:45.97 AS.seek :empty @18:34:36.71 AFV.select :carrierSolution @18:34:36.82 delay 2 seconds @18:34:38.85 AS.reconfigure ASconfig @18:34:38.90 AS.seek 0.5ml @18:35:07.96 delay 1 second @18:35:08.98 AFV.select :holdingCoil @18:35:09.08 Building Slug @18:35:09.09 ATV.dial :ARV @18:35:10.63 delay 2 seconds @18:35:12.66 ARV.dial :air1 @18:35:13.05 delay 2 seconds @18:35:15.14 AS.reconfigure ASslow2 @18:35:15.19 AS.seek 0.506ml @18:35:21.63 delay 2 seconds @18:35:23.65 ARV.dial :MM1 @18:35:24.21 delay 2 seconds @18:35:26.24 Adding MM1 @18:35:26.31 AS.seek 0.524ml @18:35:44.68 delay 25 seconds @18:36:09.71 ARV.dial :PP1 @18:36:10.09 Adding PP1 @18:36:10.10 delay 2 seconds @18:36:12.19 AS.seek 0.53ml @18:36:18.60 delay 10 seconds @18:36:28.63 ARV.dial :air1 @18:36:29.01 adding ARV air @18:36:29.03 delay 2 seconds @18:36:31.11 AS.seek 0.536ml @18:36:37.26 delay 5 seconds @18:36:42.29 ARV.dial :carrier @18:36:42.68 delay 7 seconds @18:36:49.77 AS.seek 0.594ml @18:37:48.31 delay 5 seconds @18:37:53.34 ATV.dial :ASV @18:37:53.73 delay 2 seconds @18:37:55.82 AS.seek 0.6ml @18:38:01.98 delay 4 seconds @18:38:06.01 ATV.dial ARV @18:38:06.53 delay 2 seconds @18:38:08.56 ARV.dial :carrier @18:38:08.57 delay 2 seconds @18:38:10.66 AS.seek 0.65ml @18:39:01.18 delay 4 seconds @18:39:05.21 ATV.dial PCR @18:39:05.79 ASV.dial :waste1 @18:39:06.50 ARV.dial :air1 @18:39:06.90 Positioning Slug by 0.324ml @18:39:06.99 AS.seek 0.326ml @18:44:33.00 delay 5 seconds @18:44:38.03 ATV.dial ARV @18:44:38.77 ASV.dial :PCRlock @18:44:39.16 delay 5 seconds @18:44:44.19 Running NM Profile60C300 thermocycling @18:44:44.20 PCR.program Profile60C300 @18:44:44.31 Profile60C300 should complete in < 1:59:25 @18:44:44.32 PCR.run 2:34:16.25 @20:19:59.14 PCR.off @20:19:59.81 PCR for NM completed -- closed /var/log/esp/NM20may2218h300ml.pcr @20:19:59.88 Cleaning PCR @20:19:59.89 AFV.select :carrierSolution @20:20:00.00 delay 2 seconds @20:20:02.03 AS.reconfigure ASconfig @20:20:02.08 AS.seek 0.5ml @20:20:12.37 delay 1 second @20:20:13.39 AFV.select :holdingCoil @20:20:13.49 ASV.dial :waste1 @20:20:13.87 ATV.dial :air3 @20:20:15.09 AS.reconfigure ASslow1 @20:20:15.14 AS.seek 0.51ml @20:20:17.42 delay 2 seconds @20:20:19.44 ATV.dial :bleach @20:20:20.05 AS.seek 0.81ml @20:21:20.62 delay 3 seconds @20:21:23.64 ATV.dial :air3 @20:21:24.20 delay 3 seconds @20:21:27.30 AS.seek 0.835ml @20:21:32.70 delay 3 seconds @20:21:35.73 ATV.dial :bleach @20:21:36.34 AS.seek 0.82ml @20:21:39.57 delay 3 seconds @20:21:42.59 ARV.dial :waste @20:21:43.18 delay 3 seconds @20:21:46.21 ATV.dial :ARV @20:21:47.05 AS.reconfigure ASconfig @20:21:47.10 AS.seek 0.47ml @20:22:07.54 AFV.select :carrierSolution @20:22:07.65 delay 2 seconds @20:22:09.69 AS.seek 1ml @20:22:40.48 delay 1 second @20:22:41.50 AFV.select :holdingCoil @20:22:41.60 delay 1 second @20:22:42.63 AS.reconfigure ASfast @20:22:42.68 AS.seek 0ml @20:23:10.73 AFV.select :carrierSolution @20:23:10.84 delay 2 seconds @20:23:12.87 AS.reconfigure ASconfig @20:23:12.92 AS.seek 1ml @20:24:10.70 delay 1 second @20:24:11.72 AFV.select :holdingCoil @20:24:11.82 delay 1 second @20:24:12.85 AS.reconfigure ASfast @20:24:12.90 AS.seek 0ml @20:24:40.26 AFV.select :carrierSolution @20:24:40.37 delay 2 seconds @20:24:42.40 AS.reconfigure ASconfig @20:24:42.45 AS.seek 1ml @20:25:40.24 delay 1 second @20:25:41.26 AFV.select :holdingCoil @20:25:41.36 delay 1 second @20:25:42.39 AS.reconfigure ASfast @20:25:42.44 AS.seek 0ml @20:26:09.76 ARV.dial between carrier and air1 @20:26:10.24 ATV.dial :air3 @20:26:11.47 AS.reconfigure ASslow1 @20:26:11.52 AS.seek 0.01ml @20:26:13.79 delay 2 seconds @20:26:15.81 ATV.dial :bleach @20:26:16.42 AS.seek 0.81ml @20:28:57.54 delay 3 seconds @20:29:00.57 ATV.dial :air3 @20:29:01.11 delay 3 seconds @20:29:04.20 AS.seek 0.835ml @20:29:09.50 delay 3 seconds @20:29:12.53 ATV.dial :bleach @20:29:13.13 AS.seek 0.82ml @20:29:16.39 delay 3 seconds @20:29:19.42 ATV.dial PCR @20:29:19.81 AS.reconfigure ASconfig @20:29:19.86 AS.seek :empty @20:30:07.31 PCR.program :bleachProfile @20:30:07.37 PCR program should complete in < 2:45 @20:30:07.38 PCR.run 10:00 @20:33:18.30 PCR.off @20:33:18.33 AFV.select :carrierSolution @20:33:18.44 delay 2 seconds @20:33:20.48 AS.seek 1ml @20:34:18.30 delay 1 second @20:34:19.32 AFV.select :holdingCoil @20:34:19.42 delay 1 second @20:34:20.45 AS.reconfigure ASfast @20:34:20.50 AS.seek 0ml @20:34:47.81 delay 3 seconds @20:34:50.84 AFV.select :carrierSolution @20:34:50.94 delay 2 seconds @20:34:52.97 AS.reconfigure ASconfig @20:34:53.03 AS.seek 1ml @20:35:50.80 delay 1 second @20:35:51.82 AFV.select :holdingCoil @20:35:51.92 delay 1 second @20:35:52.95 AS.reconfigure ASfast @20:35:53.00 AS.seek 0ml @20:36:20.34 delay 3 seconds @20:36:23.37 AFV.select :carrierSolution @20:36:23.47 delay 2 seconds @20:36:25.50 AS.reconfigure ASconfig @20:36:25.55 AS.seek 1ml @20:37:23.34 delay 1 second @20:37:24.36 AFV.select :holdingCoil @20:37:24.46 delay 1 second @20:37:25.49 AS.reconfigure ASfast @20:37:25.54 AS.seek 0ml @20:37:52.88 delay 3 seconds @20:37:55.91 AFV.select :carrierSolution @20:37:56.01 delay 2 seconds @20:37:58.04 AS.reconfigure ASconfig @20:37:58.10 AS.seek 1ml @20:38:55.89 delay 1 second @20:38:56.91 AFV.select :holdingCoil @20:38:57.01 delay 1 second @20:38:58.04 AS.reconfigure ASfast @20:38:58.09 AS.seek 0ml @20:39:25.42 delay 3 seconds @20:39:28.45 AFV.select :carrierSolution @20:39:28.55 delay 2 seconds @20:39:30.58 AS.reconfigure ASconfig @20:39:30.63 AS.seek 1ml @20:40:28.39 delay 1 second @20:40:29.41 AFV.select :holdingCoil @20:40:29.51 delay 1 second @20:40:30.54 AS.reconfigure ASnorm @20:40:30.59 AS.seek 0ml @20:41:21.32 Setting up PCR for IPc -- writing /var/log/esp/IPc20may2220h300ml.pcr @20:41:21.33 Building PCR Slug from ASV with MM3 mix, PP6 primer @20:41:21.35 Priming with carrier @20:41:21.36 ATV.dial ARV @20:41:21.94 ARV.dial :carrier @20:41:22.29 AS.seek 0.5ml @20:41:47.82 AFV.select :carrierSolution @20:41:47.93 delay 2 seconds @20:41:49.96 AS.reconfigure ASconfig @20:41:50.01 AS.seek 1ml @20:42:19.77 delay 1 second @20:42:20.79 AFV.select :holdingCoil @20:42:20.89 delay 1 second @20:42:21.92 ATV.dial :waste @20:42:23.47 AS.reconfigure ASnorm @20:42:23.53 AS.seek :empty @20:43:14.27 AFV.select :carrierSolution @20:43:14.38 delay 2 seconds @20:43:16.41 AS.reconfigure ASconfig @20:43:16.46 AS.seek 0.5ml @20:43:45.55 delay 1 second @20:43:46.57 AFV.select :holdingCoil @20:43:46.67 Building Slug @20:43:46.68 ATV.dial :ARV @20:43:48.22 delay 2 seconds @20:43:50.25 ARV.dial :air1 @20:43:50.64 delay 2 seconds @20:43:52.73 AS.reconfigure ASslow2 @20:43:52.79 AS.seek 0.506ml @20:43:59.10 delay 2 seconds @20:44:01.13 ARV.dial :MM3 @20:44:02.11 delay 2 seconds @20:44:04.14 Adding MM3 @20:44:04.23 AS.seek 0.524ml @20:44:22.68 delay 25 seconds @20:44:47.71 ARV.dial :PP6 @20:44:48.10 Adding PP6 @20:44:48.12 delay 2 seconds @20:44:50.20 AS.seek 0.53ml @20:44:56.46 delay 10 seconds @20:45:06.49 ARV.dial :air1 @20:45:07.27 adding ARV air @20:45:07.29 delay 2 seconds @20:45:09.37 AS.seek 0.536ml @20:45:15.58 delay 5 seconds @20:45:20.61 ARV.dial :carrier @20:45:20.99 delay 7 seconds @20:45:28.09 AS.seek 0.594ml @20:46:26.58 delay 5 seconds @20:46:31.61 ATV.dial :ASV @20:46:32.01 delay 2 seconds @20:46:34.10 AS.seek 0.6ml @20:46:40.30 delay 4 seconds @20:46:44.33 ATV.dial ARV @20:46:44.85 delay 2 seconds @20:46:46.88 ARV.dial :carrier @20:46:46.89 delay 2 seconds @20:46:48.98 AS.seek 0.65ml @20:47:39.48 delay 4 seconds @20:47:43.51 ATV.dial PCR @20:47:44.10 ASV.dial :waste1 @20:47:44.11 ARV.dial :air1 @20:47:44.50 Positioning Slug by 0.324ml @20:47:44.59 AS.seek 0.326ml @20:53:10.67 delay 5 seconds @20:53:15.70 ATV.dial ARV @20:53:16.43 ASV.dial :PCRlock @20:53:16.82 delay 5 seconds @20:53:21.85 Running IPc ProfileIPc thermocycling @20:53:21.86 PCR.program ProfileIPc @20:53:21.97 ProfileIPc should complete in < 1:36:15 @20:53:21.98 PCR.run 2:05:18.75 @22:11:24.21 PCR.off @22:11:24.76 PCR for IPc completed -- closed /var/log/esp/IPc20may2220h300ml.pcr @22:11:24.84 Cleaning PCR @22:11:24.85 AFV.select :carrierSolution @22:11:24.96 delay 2 seconds @22:11:26.99 AS.reconfigure ASconfig @22:11:27.04 AS.seek 0.5ml @22:11:37.35 delay 1 second @22:11:38.37 AFV.select :holdingCoil @22:11:38.47 ASV.dial :waste1 @22:11:38.85 ATV.dial :air3 @22:11:40.07 AS.reconfigure ASslow1 @22:11:40.12 AS.seek 0.51ml @22:11:42.39 delay 2 seconds @22:11:44.42 ATV.dial :bleach @22:11:45.03 AS.seek 0.81ml @22:12:45.61 delay 3 seconds @22:12:48.64 ATV.dial :air3 @22:12:49.18 delay 3 seconds @22:12:52.27 AS.seek 0.835ml @22:12:57.55 delay 3 seconds @22:13:00.58 ATV.dial :bleach @22:13:01.18 AS.seek 0.82ml @22:13:04.49 delay 3 seconds @22:13:07.52 ARV.dial :waste @22:13:08.10 delay 3 seconds @22:13:11.13 ATV.dial :ARV @22:13:11.97 AS.reconfigure ASconfig @22:13:12.03 AS.seek 0.47ml @22:13:32.43 AFV.select :carrierSolution @22:13:32.54 delay 2 seconds @22:13:34.58 AS.seek 1ml @22:14:05.38 delay 1 second @22:14:06.40 AFV.select :holdingCoil @22:14:06.50 delay 1 second @22:14:07.53 AS.reconfigure ASfast @22:14:07.58 AS.seek 0ml @22:14:34.94 AFV.select :carrierSolution @22:14:35.05 delay 2 seconds @22:14:37.08 AS.reconfigure ASconfig @22:14:37.13 AS.seek 1ml @22:15:34.93 delay 1 second @22:15:35.95 AFV.select :holdingCoil @22:15:36.06 delay 1 second @22:15:37.09 AS.reconfigure ASfast @22:15:37.14 AS.seek 0ml @22:16:04.49 AFV.select :carrierSolution @22:16:04.60 delay 2 seconds @22:16:06.63 AS.reconfigure ASconfig @22:16:06.68 AS.seek 1ml @22:17:04.49 delay 1 second @22:17:05.51 AFV.select :holdingCoil @22:17:05.61 delay 1 second @22:17:06.64 AS.reconfigure ASfast @22:17:06.69 AS.seek 0ml @22:17:34.04 ARV.dial between carrier and air1 @22:17:34.52 ATV.dial :air3 @22:17:35.75 AS.reconfigure ASslow1 @22:17:35.80 AS.seek 0.01ml @22:17:38.13 delay 2 seconds @22:17:40.15 ATV.dial :bleach @22:17:40.76 AS.seek 0.81ml @22:20:22.63 delay 3 seconds @22:20:25.66 ATV.dial :air3 @22:20:26.20 delay 3 seconds @22:20:29.29 AS.seek 0.835ml @22:20:34.59 delay 3 seconds @22:20:37.62 ATV.dial :bleach @22:20:38.23 AS.seek 0.82ml @22:20:41.54 delay 3 seconds @22:20:44.57 ATV.dial PCR @22:20:44.95 AS.reconfigure ASconfig @22:20:45.00 AS.seek :empty @22:21:32.46 PCR.program :bleachProfile @22:21:32.52 PCR program should complete in < 2:45 @22:21:32.53 PCR.run 10:00 @22:24:43.44 PCR.off @22:24:43.47 AFV.select :carrierSolution @22:24:43.58 delay 2 seconds @22:24:45.62 AS.seek 1ml @22:25:43.42 delay 1 second @22:25:44.44 AFV.select :holdingCoil @22:25:44.54 delay 1 second @22:25:45.57 AS.reconfigure ASfast @22:25:45.62 AS.seek 0ml @22:26:12.96 delay 3 seconds @22:26:15.99 AFV.select :carrierSolution @22:26:16.09 delay 2 seconds @22:26:18.12 AS.reconfigure ASconfig @22:26:18.18 AS.seek 1ml @22:27:15.92 delay 1 second @22:27:16.94 AFV.select :holdingCoil @22:27:17.04 delay 1 second @22:27:18.07 AS.reconfigure ASfast @22:27:18.12 AS.seek 0ml @22:27:45.45 delay 3 seconds @22:27:48.48 AFV.select :carrierSolution @22:27:48.58 delay 2 seconds @22:27:50.61 AS.reconfigure ASconfig @22:27:50.67 AS.seek 1ml @22:28:48.45 delay 1 second @22:28:49.47 AFV.select :holdingCoil @22:28:49.57 delay 1 second @22:28:50.60 AS.reconfigure ASfast @22:28:50.65 AS.seek 0ml @22:29:18.01 delay 3 seconds @22:29:21.04 AFV.select :carrierSolution @22:29:21.14 delay 2 seconds @22:29:23.17 AS.reconfigure ASconfig @22:29:23.23 AS.seek 1ml @22:30:21.06 delay 1 second @22:30:22.08 AFV.select :holdingCoil @22:30:22.18 delay 1 second @22:30:23.21 AS.reconfigure ASfast @22:30:23.26 AS.seek 0ml @22:30:50.61 delay 3 seconds @22:30:53.64 AFV.select :carrierSolution @22:30:53.74 delay 2 seconds @22:30:55.77 AS.reconfigure ASconfig @22:30:55.82 AS.seek 1ml @22:31:53.58 delay 1 second @22:31:54.60 AFV.select :holdingCoil @22:31:54.70 delay 1 second @22:31:55.73 AS.reconfigure ASnorm @22:31:55.78 AS.seek 0ml @22:32:46.52 Setting up PCR for IPc2 -- writing /var/log/esp/IPc220may2222h300ml.pcr @22:32:46.54 Building PCR Slug from ASV with MM2 mix, PP3 primer @22:32:46.55 Priming with carrier @22:32:46.56 ATV.dial ARV @22:32:47.14 ARV.dial :carrier @22:32:47.49 AS.seek 0.5ml @22:33:13.05 AFV.select :carrierSolution @22:33:13.16 delay 2 seconds @22:33:15.19 AS.reconfigure ASconfig @22:33:15.24 AS.seek 1ml @22:33:44.28 delay 1 second @22:33:45.30 AFV.select :holdingCoil @22:33:45.40 delay 1 second @22:33:46.43 ATV.dial :waste @22:33:47.98 AS.reconfigure ASnorm @22:33:48.03 AS.seek :empty @22:34:38.78 AFV.select :carrierSolution @22:34:38.89 delay 2 seconds @22:34:40.92 AS.reconfigure ASconfig @22:34:40.97 AS.seek 0.5ml @22:35:10.05 delay 1 second @22:35:11.07 AFV.select :holdingCoil @22:35:11.17 Building Slug @22:35:11.18 ATV.dial :ARV @22:35:12.72 delay 2 seconds @22:35:14.75 ARV.dial :air1 @22:35:15.13 delay 2 seconds @22:35:17.22 AS.reconfigure ASslow2 @22:35:17.27 AS.seek 0.506ml @22:35:23.50 delay 2 seconds @22:35:25.52 ARV.dial :MM2 @22:35:26.92 delay 2 seconds @22:35:28.95 Adding MM2 @22:35:29.03 AS.seek 0.524ml @22:35:47.30 delay 25 seconds @22:36:13.04 ARV.dial :PP3 @22:36:13.42 Adding PP3 @22:36:13.44 delay 2 seconds @22:36:15.52 AS.seek 0.53ml @22:36:21.75 delay 10 seconds @22:36:31.78 ARV.dial :air2 @22:36:32.16 adding ARV air @22:36:32.18 delay 2 seconds @22:36:34.26 AS.seek 0.536ml @22:36:40.53 delay 5 seconds @22:36:45.56 ARV.dial :carrier @22:36:46.77 delay 7 seconds @22:36:53.87 AS.seek 0.594ml @22:37:52.41 delay 5 seconds @22:37:57.44 ATV.dial :ASV @22:37:57.83 delay 2 seconds @22:37:59.92 AS.seek 0.6ml @22:38:06.34 delay 4 seconds @22:38:10.37 ATV.dial ARV @22:38:10.89 delay 2 seconds @22:38:12.92 ARV.dial :carrier @22:38:12.93 delay 2 seconds @22:38:15.02 AS.seek 0.65ml @22:39:05.47 delay 4 seconds @22:39:09.50 ATV.dial PCR @22:39:10.08 ASV.dial :waste1 @22:39:10.09 ARV.dial :air1 @22:39:10.50 Positioning Slug by 0.324ml @22:39:10.59 AS.seek 0.326ml @22:44:36.64 delay 5 seconds @22:44:41.67 ATV.dial ARV @22:44:42.41 ASV.dial :PCRlock @22:44:42.80 delay 5 seconds @22:44:47.83 Running IPc2 ProfileIPc thermocycling @22:44:47.84 PCR.program ProfileIPc @22:44:47.94 ProfileIPc should complete in < 1:36:15 @22:44:47.95 PCR.run 2:05:18.75 @00:02:50.77CEST23-May-20 PCR.off @00:02:51.34 PCR for IPc2 completed -- closed /var/log/esp/IPc220may2222h300ml.pcr @00:02:51.35 Full MFB Decontamination @00:02:51.37 Decon extraction column and decon AIV @00:02:51.39 SPE.hold 2:46:40,55C @00:02:51.44 ASV.dial :SPEdelivery @00:02:52.07 delay 20 seconds @00:03:12.10 AFV.select :carrierSolution @00:03:12.20 delay 2 seconds @00:03:14.23 AS.reconfigure ASconfig @00:03:14.28 AS.seek 0.5ml @00:03:24.62 delay 1 second @00:03:25.64 AFV.select :holdingCoil @00:03:25.74 ATV.dial :air1 @00:03:27.56 AS.reconfigure ASslow1 @00:03:27.61 AS.seek 0.52ml @00:03:31.90 ATV.dial :ASV @00:03:33.60 ASV.dial :SPErecovery @00:03:34.25 AS.seek 0.62ml @00:03:54.65 ATV.dial :waste @00:03:56.57 AS.reconfigure ASnorm @00:03:56.62 AS.seek :empty @00:04:28.23 AFV.select :holdingCoil @00:04:28.24 ATV.dial :waste @00:04:28.26 AS.reconfigure ASconfig @00:04:28.31 AS.seek 0ml @00:04:28.60 AFV.select :carrierSolution @00:04:28.71 delay 2 seconds @00:04:30.75 AS.seek 0.5ml @00:04:59.78 delay 1 second @00:05:00.80 AFV.select :holdingCoil @00:05:00.90 ATV.dial :air3 @00:05:01.60 AS.reconfigure ASslow1 @00:05:01.65 AS.seek 0.52ml @00:05:05.95 ATV.dial :air3 @00:05:06.04 AS.seek 0.53ml @00:05:08.29 delay 2 seconds @00:05:10.31 ATV.dial :bleach @00:05:10.78 AS.seek 0.71ml @00:05:47.25 delay 3 seconds @00:05:50.28 ATV.dial :air3 @00:05:50.86 delay 3 seconds @00:05:53.95 AS.seek 0.735ml @00:05:59.24 delay 3 seconds @00:06:02.27 ATV.dial :bleach @00:06:02.89 AS.seek 0.72ml @00:06:06.20 delay 3 seconds @00:06:09.23 ATV.dial ASV @00:06:10.26 ASV.dial :SPErecovery @00:06:10.34 AS.seek 0.365ml @00:07:22.00 ATV.dial :waste @00:07:23.67 AS.reconfigure ASnorm @00:07:23.72 AS.seek :empty @00:07:42.50 ATV.dial :air3 @00:07:43.18 AS.reconfigure ASslow1 @00:07:43.23 AS.seek 0.009ml @00:07:45.57 delay 2 seconds @00:07:47.59 ATV.dial :bleach @00:07:48.07 AS.seek 0.579ml @00:09:42.97 delay 3 seconds @00:09:46.00 ATV.dial :air3 @00:09:46.53 delay 3 seconds @00:09:49.62 AS.seek 0.604ml @00:09:54.91 delay 3 seconds @00:09:57.94 ATV.dial :bleach @00:09:58.54 AS.seek 0.589ml @00:10:01.84 delay 3 seconds @00:10:04.86 ATV.dial :ASV @00:10:05.86 ASV.dial :wasteR @00:10:06.24 AS.seek 0ml @00:12:04.95 delay 1:00 @00:13:04.98 ATV.dial ASV @00:13:04.99 AFV.select :carrierSolution @00:13:05.09 delay 2 seconds @00:13:07.12 AS.reconfigure ASconfig @00:13:07.17 AS.seek 1ml @00:14:05.70 delay 1 second @00:14:06.72 AFV.select :holdingCoil @00:14:06.82 delay 1 second @00:14:07.85 ASV.dial :wasteR @00:14:07.87 AS.reconfigure ASslow1 @00:14:07.91 AS.seek 0ml @00:17:29.28 delay 10 seconds @00:17:39.31 ATV.dial :air3 @00:17:40.74 AS.seek 0.01ml @00:17:43.02 delay 2 seconds @00:17:45.04 ATV.dial :bleach @00:17:45.65 AS.seek 0.58ml @00:19:40.55 delay 3 seconds @00:19:43.58 ATV.dial :air3 @00:19:44.11 delay 3 seconds @00:19:47.20 AS.seek 0.605ml @00:19:52.52 delay 3 seconds @00:19:55.55 ATV.dial :bleach @00:19:56.15 AS.seek 0.59ml @00:19:59.44 delay 3 seconds @00:20:02.47 ATV.dial ASV @00:20:03.46 ASV.dial :SPEdelivery @00:20:03.84 AS.seek 0ml @00:22:02.71 delay 1:00 @00:23:02.74 AFV.select :carrierSolution @00:23:02.84 delay 2 seconds @00:23:04.87 AS.reconfigure ASconfig @00:23:04.92 AS.seek 1ml @00:24:02.69 delay 1 second @00:24:03.71 AFV.select :holdingCoil @00:24:03.81 delay 1 second @00:24:04.84 ATV.dial ASV @00:24:04.85 ASV.dial :SPEdelivery @00:24:04.87 AS.reconfigure ASslow1 @00:24:04.91 AS.seek 0ml @00:27:26.29 delay 10 seconds @00:27:36.32 ATV.dial :air2 @00:27:37.12 AS.reconfigure ASslow0 @00:27:37.16 AS.seek 0.8ml @00:28:57.94 delay 3 seconds @00:29:00.97 ATV.dial :ASV @00:29:01.90 ASV.dial :SPEdelivery @00:29:01.92 AS.reconfigure ASslow1 @00:29:01.97 AS.seek 0.03ml @00:31:37.07 delay 20 seconds @00:31:57.10 ATV.dial :waste,via: :air3 @00:31:58.73 AS.reconfigure ASconfig @00:31:58.78 AS.seek :empty @00:32:00.84 SPE.coast @00:32:00.93 Removing Bleach from Mixing Coil @00:32:00.95 AFV.select :carrierSolution @00:32:01.05 delay 2 seconds @00:32:03.09 AS.seek 0.5ml @00:32:32.19 delay 1 second @00:32:33.21 AFV.select :holdingCoil @00:32:33.31 ATV.dial :ASV @00:32:35.04 ASV.dial :SPErecovery @00:32:35.69 AS.reconfigure ASslow1 @00:32:35.74 AS.seek 0.73ml @00:33:22.27 ASV.dial :wasteD @00:33:22.90 AS.reconfigure ASnorm @00:33:22.95 AS.seek 0ml @00:34:00.09 AFV.select :carrierSolution @00:34:00.20 delay 2 seconds @00:34:02.23 AS.reconfigure ASconfig @00:34:02.28 AS.seek 0.5ml @00:34:31.35 delay 1 second @00:34:32.37 AFV.select :holdingCoil @00:34:32.47 ATV.dial ASV @00:34:32.48 ASV.dial :SPErecovery @00:34:33.11 AS.reconfigure ASslow0 @00:34:33.16 AS.seek 0.3ml @00:34:53.59 ATV.dial :air2 @00:34:54.45 AS.reconfigure ASslow1 @00:34:54.50 AS.seek 0.32ml @00:34:58.81 ATV.dial ASV @00:34:59.81 AS.reconfigure ASslow0 @00:34:59.86 AS.seek 0.57ml @00:35:25.30 ASV.dial :wasteD @00:35:25.85 AS.reconfigure ASnorm @00:35:25.90 AS.seek 0ml @00:35:54.98 AFV.select :carrierSolution @00:35:55.09 delay 2 seconds @00:35:57.12 AS.reconfigure ASconfig @00:35:57.17 AS.seek 0.5ml @00:36:26.20 delay 1 second @00:36:27.22 AFV.select :holdingCoil @00:36:27.32 ATV.dial ASV @00:36:27.33 ASV.dial :SPErecovery @00:36:27.95 AS.reconfigure ASslow0 @00:36:28.00 AS.seek 0.3ml @00:36:48.46 ATV.dial :air2 @00:36:50.15 AS.reconfigure ASslow1 @00:36:50.20 AS.seek 0.32ml @00:36:54.49 ATV.dial ASV @00:36:55.49 AS.reconfigure ASslow0 @00:36:55.55 AS.seek 0.57ml @00:37:20.99 ASV.dial :wasteD @00:37:21.55 AS.reconfigure ASnorm @00:37:21.60 AS.seek 0ml @00:37:50.67 ATV.dial :air2 @00:37:51.61 AS.reconfigure ASslow0 @00:37:51.66 AS.seek 0.3ml @00:38:22.20 ATV.dial :ASV @00:38:23.13 ASV.dial :wasteD @00:38:23.15 AS.reconfigure ASconfig @00:38:23.20 AS.seek 0.05ml @00:38:37.90 ATV.dial :waste @00:38:39.64 AS.seek 0ml @00:38:42.81 ASV.dial :SPEdelivery @00:38:43.76 deconLysateINT @00:38:43.77 ATV.dial :air3 @00:38:44.45 AS.reconfigure ASslow1 @00:38:44.50 AS.seek 0.01ml @00:38:46.79 delay 2 seconds @00:38:48.81 ATV.dial :bleach @00:38:49.27 AS.seek 0.96ml @00:42:00.57 delay 3 seconds @00:42:03.60 ATV.dial :air3 @00:42:04.14 delay 3 seconds @00:42:07.23 AS.seek 0.985ml @00:42:12.53 delay 3 seconds @00:42:15.56 ATV.dial :bleach @00:42:16.17 AS.seek 0.97ml @00:42:19.51 delay 3 seconds @00:42:22.54 ATV.dial AIV @00:42:23.71 AIV.dial :coilToMFB @00:42:23.73 AWV.select :waste @00:42:23.74 AFV.select :carrierSolution @00:42:23.85 delay 2 seconds @00:42:25.88 AS.reconfigure ASconfig @00:42:25.93 AS.seek 1ml @00:42:27.96 delay 1 second @00:42:28.98 AFV.select :holdingCoil @00:42:29.08 delay 1 second @00:42:30.11 AS.reconfigure ASslow0 @00:42:30.16 AS.seek :empty @00:44:11.12 AFV.select :carrierSolution @00:44:11.23 delay 2 seconds @00:44:13.26 AS.reconfigure ASconfig @00:44:13.31 AS.seek 1ml @00:45:11.12 delay 1 second @00:45:12.14 AFV.select :holdingCoil @00:45:12.24 delay 1 second @00:45:13.27 AS.reconfigure ASslow0 @00:45:13.32 AS.seek :empty @00:46:54.28 AFV.select :carrierSolution @00:46:54.39 delay 2 seconds @00:46:56.42 AS.reconfigure ASconfig @00:46:56.47 AS.seek 1ml @00:47:54.28 delay 1 second @00:47:55.30 AFV.select :holdingCoil @00:47:55.40 delay 1 second @00:47:56.43 AS.reconfigure ASslow0 @00:47:56.48 AS.seek :empty @00:49:37.44 ATV.dial :air2 @00:49:38.10 AS.reconfigure ASslow1 @00:49:38.15 AS.seek 0.009ml @00:49:40.45 AS.reconfigure ASslow0 @00:49:40.50 AS.seek 1ml @00:51:20.42 ATV.dial AIV @00:51:21.17 AS.seek 0.02ml @00:53:00.05 ATV.dial :waste @00:53:01.62 AS.seek 0ml @00:53:03.92 AWV.select :air @00:53:04.09 Cleaning PCR @00:53:04.10 AFV.select :carrierSolution @00:53:04.20 delay 2 seconds @00:53:06.23 AS.reconfigure ASconfig @00:53:06.28 AS.seek 0.5ml @00:53:35.31 delay 1 second @00:53:36.33 AFV.select :holdingCoil @00:53:36.43 ASV.dial :waste1 @00:53:36.80 ATV.dial :air3 @00:53:37.46 AS.reconfigure ASslow1 @00:53:37.51 AS.seek 0.51ml @00:53:39.77 delay 2 seconds @00:53:41.79 ATV.dial :bleach @00:53:42.24 AS.seek 0.81ml @00:54:42.83 delay 3 seconds @00:54:45.86 ATV.dial :air3 @00:54:47.08 delay 3 seconds @00:54:50.18 AS.seek 0.835ml @00:54:55.48 delay 3 seconds @00:54:58.51 ATV.dial :bleach @00:54:59.12 AS.seek 0.82ml @00:55:02.45 delay 3 seconds @00:55:05.48 ARV.dial :waste @00:55:06.06 delay 3 seconds @00:55:09.09 ATV.dial :ARV @00:55:09.95 AS.reconfigure ASconfig @00:55:10.00 AS.seek 0.47ml @00:55:30.44 AFV.select :carrierSolution @00:55:30.55 delay 2 seconds @00:55:32.59 AS.seek 1ml @00:56:03.38 delay 1 second @00:56:04.40 AFV.select :holdingCoil @00:56:04.50 delay 1 second @00:56:05.53 AS.reconfigure ASfast @00:56:05.58 AS.seek 0ml @00:56:32.92 AFV.select :carrierSolution @00:56:33.03 delay 2 seconds @00:56:35.06 AS.reconfigure ASconfig @00:56:35.11 AS.seek 1ml @00:57:32.91 delay 1 second @00:57:33.93 AFV.select :holdingCoil @00:57:34.03 delay 1 second @00:57:35.06 AS.reconfigure ASfast @00:57:35.11 AS.seek 0ml @00:58:02.45 AFV.select :carrierSolution @00:58:02.56 delay 2 seconds @00:58:04.59 AS.reconfigure ASconfig @00:58:04.64 AS.seek 1ml @00:59:02.44 delay 1 second @00:59:03.46 AFV.select :holdingCoil @00:59:03.56 delay 1 second @00:59:04.59 AS.reconfigure ASfast @00:59:04.64 AS.seek 0ml @00:59:31.99 ARV.dial between carrier and air1 @00:59:32.47 ATV.dial :air3 @00:59:33.70 AS.reconfigure ASslow1 @00:59:33.75 AS.seek 0.01ml @00:59:36.05 delay 2 seconds @00:59:38.07 ATV.dial :bleach @00:59:38.68 AS.seek 0.81ml @01:02:19.80 delay 3 seconds @01:02:22.83 ATV.dial :air3 @01:02:23.36 delay 3 seconds @01:02:26.45 AS.seek 0.835ml @01:02:31.73 delay 3 seconds @01:02:34.76 ATV.dial :bleach @01:02:35.37 AS.seek 0.82ml @01:02:38.66 delay 3 seconds @01:02:41.69 ATV.dial PCR @01:02:42.08 AS.reconfigure ASconfig @01:02:42.13 AS.seek :empty @01:03:29.61 PCR.program :bleachProfile @01:03:29.67 PCR program should complete in < 2:45 @01:03:29.68 PCR.run 10:00 @01:06:52.71 PCR.off @01:06:52.74 AFV.select :carrierSolution @01:06:52.85 delay 2 seconds @01:06:54.89 AS.seek 1ml @01:07:52.71 delay 1 second @01:07:53.73 AFV.select :holdingCoil @01:07:53.83 delay 1 second @01:07:54.86 AS.reconfigure ASfast @01:07:54.91 AS.seek 0ml @01:08:22.26 delay 3 seconds @01:08:25.29 AFV.select :carrierSolution @01:08:25.39 delay 2 seconds @01:08:27.42 AS.reconfigure ASconfig @01:08:27.47 AS.seek 1ml @01:09:25.24 delay 1 second @01:09:26.26 AFV.select :holdingCoil @01:09:26.36 delay 1 second @01:09:27.39 AS.reconfigure ASfast @01:09:27.44 AS.seek 0ml @01:09:54.77 delay 3 seconds @01:09:57.80 AFV.select :carrierSolution @01:09:57.90 delay 2 seconds @01:09:59.93 AS.reconfigure ASconfig @01:09:59.99 AS.seek 1ml @01:10:57.74 delay 1 second @01:10:58.76 AFV.select :holdingCoil @01:10:58.86 delay 1 second @01:10:59.89 AS.reconfigure ASfast @01:10:59.94 AS.seek 0ml @01:11:27.29 delay 3 seconds @01:11:30.32 AFV.select :carrierSolution @01:11:30.42 delay 2 seconds @01:11:32.45 AS.reconfigure ASconfig @01:11:32.50 AS.seek 1ml @01:12:31.01 delay 1 second @01:12:32.04 AFV.select :holdingCoil @01:12:32.14 delay 1 second @01:12:33.17 AS.reconfigure ASfast @01:12:33.22 AS.seek 0ml @01:13:00.55 delay 3 seconds @01:13:03.58 AFV.select :carrierSolution @01:13:03.68 delay 2 seconds @01:13:05.71 AS.reconfigure ASconfig @01:13:05.77 AS.seek 1ml @01:14:03.59 delay 1 second @01:14:04.61 AFV.select :holdingCoil @01:14:04.71 delay 1 second @01:14:05.74 AS.reconfigure ASnorm @01:14:05.79 AS.seek 0ml @01:14:56.50 AFV.select :carrierSolution @01:14:56.61 delay 2 seconds @01:14:58.65 AS.reconfigure ASconfig @01:14:58.74 AS.seek 1ml @01:15:56.54 delay 1 second @01:15:57.56 AFV.select :holdingCoil @01:15:57.66 delay 1 second @01:15:58.69 ATV.dial :waste @01:15:59.85 AS.reconfigure ASnorm @01:15:59.89 AS.seek 0.5ml @01:16:25.46 ATV.dial :waste2 @01:16:26.68 AS.seek 0ml @01:16:52.20 AS.coast @01:16:52.25 SS.coast @01:16:52.28 CC.coast @01:16:52.33 CS.coast @01:16:52.37 PC.coast @01:16:52.41 PS.coast @01:16:52.45 Forearm.coast @01:16:52.49 Elbow.coast @01:16:52.88 Gate.power :core,:OFF @01:16:52.90 Gate.power :raw,:OFF @01:16:52.91 Shutdown @01:16:52.92 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @10:00:51.39
Powering core bus @10:00:56.32 Gate.power :raw,:ON @10:00:56.98 SPE.coast @10:00:57.04 SPE.configure SPEconfig @10:00:57.14 AS.configure ASconfig @10:00:57.24 SS.configure SSconfig @10:00:57.34 CC.configure CCconfig @10:00:57.43 CS.configure CSconfig @10:00:57.53 PC.configure PCconfig @10:00:57.64 PS.configure PSconfig @10:00:57.76 Forearm.configure LooseForearm @10:00:57.86 Elbow.configure LooseElbow @10:00:57.98 SC.configure SCloose @10:00:58.08 SE.configure SEconfig @10:00:58.12 CH.coast @10:00:58.18 CH.configure CHconfig @10:00:58.21 PH.coast @10:00:58.27 PH.configure PHconfig @10:00:58.31 PCR.off @10:00:58.34 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:58.37 PCR.caltemp= 2.8814,0.0372,0 @10:00:58.39 All dwarf controllers running firmware version 3.75 @10:00:58.51 Valve.reset @10:00:58.68 PCR.off @10:00:58.71 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:58.74 PCR.caltemp= 2.8814,0.0372,0 @10:00:59.52 Intake.select :close @10:00:59.61 Exhaust.select :close @10:00:59.63 CTV.dial :air @10:00:59.71 PSV.dial :air @10:00:59.84 CSV.dial :waste1 @10:00:59.88
Forearm.reconfigure LooseForearm @10:00:59.99 Forearm.home.seek :retract @10:01:00.02 SSV.dial :spit,avoiding: :exhaust @10:01:00.23 PS.reconfigure PSconfig @10:01:00.29 PS.home.seek 0ml @10:01:00.79 Hand.close @10:01:00.82
Elbow.jog 1500 @10:01:00.96 CS.reconfigure CSfast @10:01:00.99 SS.reconfigure SSconfig @10:01:01.09 CS.home.seek 0ml @10:01:01.12 SS.home.seek 0ml @10:01:01.60
Elbow.reconfigure LooseElbow @10:01:01.65 Elbow.home.seek :clear @10:01:04.99 SE.reconfigure SEconfig @10:01:05.04 SE.home.seek :down @10:01:05.77 SC.reconfigure SCsloppy @10:01:05.82 SC.home.seek :initial @10:01:09.36 PS.coast @10:01:09.86
Forearm.seek :retract @10:01:09.94 Elbow.seek :hammer @10:01:11.15 Forearm.reconfigure TightForearm @10:01:11.20 Forearm.seek 5 + 350 counts @10:01:12.28 Forearm.seek :retract @10:01:13.48 PC.reconfigure PCconfig @10:01:13.49
CC.reconfigure CCconfig @10:01:13.58 CC.home.seek :open @10:01:13.63 PC.home.seek :open @10:01:17.18
Phase [stdphase] @10:01:17.19 GoodNews.email "At 10:01:17 began in tube 3: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:150\n{1 => 22, 2 => 9, 3 => 13}\nCan@10:01:17, 9.3C, 48% humidity, 14.4psia, 13.412V, 0.468A, 0.468A avg, 6.28W",Subject: "Started 20invasivedeployfinal phase stdphase" @10:01:17.23CEST23-May-20
===> STARTED Superphase (phase #13) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @10:01:17.25 Starting initialPurge @10:01:17.26 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:01:17.34 Hand.open @10:01:18.04 Elbow.reconfigure TightElbow @10:01:18.09 Elbow.seek FlushPuck::Garage @10:01:19.99 Elbow.reconfigure LooseElbow @10:01:20.10 Forearm.reconfigure LooseForearm @10:01:20.15 Forearm.seek :garage @10:01:21.13 Hand.close @10:01:21.93 Forearm.seek :retract @10:01:22.97 Elbow.reconfigure TightElbow @10:01:23.01 Elbow.seek CC @10:01:26.04 Elbow.reconfigure LooseElbow @10:01:26.25 Forearm.reconfigure TightForearm @10:01:26.35 Forearm.seek CC @10:01:28.11 CC.seek :closed @10:01:32.56 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.07 @10:01:32.76 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[11:27->20] @10:01:32.83 Hand.open @10:01:33.70 Forearm.reconfigure LooseForearm @10:01:33.78 Forearm.seek :retract @10:01:34.89 Flushing the Collection Valve::Manifold @10:01:35.17 CSV.dial CSR @10:01:36.73 Pulling 5ml of CSR.flush @10:01:36.75 CSR.select :flush @10:01:36.97 CS.reconfigure CSconfig @10:01:37.07 CS.seek 5ml @10:01:50.99 delay 5 seconds @10:01:56.03 CSR.select :air @10:01:56.34 CS.reconfigure CSfast @10:01:56.44 CS.seek 7.501ml @10:02:03.31 CSV.dial :waste @10:02:04.74 CS.reconfigure CSconfig @10:02:04.84 CS.seek 0ml @10:02:24.99 Purging external Intake @10:02:25.12 CSV.dial :flush @10:02:25.60 CS.reconfigure CSfast @10:02:25.65 CS.seek 10ml @10:02:49.55 delay 5 seconds @10:02:54.58 CSV.dial CBV @10:02:54.61
CBV.dial CSV @10:02:55.46 CTV.dial :intake @10:02:56.24 Intake.select :open @10:02:56.36 CS.seek 0ml @10:03:21.90 Intake.select :close @10:03:22.01 CTV.dial :air @10:03:22.82 Flushing upper manifold @10:03:22.95 CSV.dial :flush @10:03:24.36 CS.seek 5ml @10:03:36.90 delay 5 seconds @10:03:41.93 CSV.dial :air @10:03:42.48 CS.seek 7ml @10:03:48.09 CSV.dial CBV @10:03:48.13
CBV.dial CSV @10:03:49.21 CTV.dial :air @10:03:49.23 CS.seek 0ml @10:04:06.31 delay 7 seconds @10:04:13.35 CS.seek 3ml @10:04:21.34 CSV.dial :waste @10:04:21.83 CS.seek 0ml @10:04:29.89 CSV.dial :flush @10:04:30.36 CS.seek 3ml @10:04:38.24 delay 5 seconds @10:04:43.27 CSV.dial :air @10:04:43.83 CS.seek 5ml @10:04:49.43 CSV.dial :air @10:04:49.45 CS.seek 0ml @10:05:02.15 delay 7 seconds @10:05:09.19 CS.seek 3ml @10:05:17.21 CSV.dial :waste @10:05:18.00 CS.seek 0ml @10:05:25.93 Garaging FlushPuck in Collection Clamp @10:05:26.01 Elbow.reconfigure TightElbow @10:05:26.06 Elbow.seek CC @10:05:26.32 Elbow.reconfigure LooseElbow @10:05:26.43 Forearm.seek CC @10:05:27.44 Hand.close @10:05:28.05 CC.seek :open @10:05:32.41 Forearm.seek :retract @10:05:33.41 Elbow.reconfigure TightElbow @10:05:33.46 Elbow.seek :garage @10:05:36.62 Elbow.reconfigure LooseElbow @10:05:36.72 Forearm.reconfigure TightForearm @10:05:36.77 Forearm.seek :garage @10:05:37.91 Hand.open @10:05:38.62 Forearm.reconfigure LooseForearm @10:05:38.66 Forearm.seek :retract @10:05:39.77 Starting mfb @10:05:39.79 pcrBAC sampling at most 1500ml, wcr at most 1500ml @10:05:39.83 BACfans.email "Started mfb in tube 3 at 10:05:39CEST23-May-20\n{1 => 22, 2 => 9, 3 => 13}\nCan@10:05:36, 9.4C, 48% humidity, 14.4psia, 13.392V, 0.474A, 0.474A avg, 6.35W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @10:05:39.93
Loading sh1 puck from tube 3 into Collection Clamp @10:05:40.02 Readying the MFB @10:05:40.09 Homing MFB Syringe @10:05:40.10 AFV.select :holdingCoil @10:05:40.34 ATV.dial :air2 @10:05:40.36
SC.reconfigure SCtight @10:05:40.48 SC.seek 3 @10:05:40.98 AS.reconfigure ASconfig @10:05:41.07 AS.home.seek :home @10:05:42.60
SC.reconfigure SCloose @10:05:42.86 Elbow.reconfigure TightElbow @10:05:42.93 Elbow.seek 3 @10:05:45.97 Forearm.reconfigure TightForearm @10:05:46.02 Forearm.seek 3 + 700 counts @10:05:46.73 AFV.select :carrierSolution @10:05:46.88 delay 2 seconds @10:05:47.24
SE.reconfigure SEpick @10:05:47.43 SE.seek beamBreak @10:05:48.97 AS.seek 1ml @10:05:49.52
SE.configure SEconfig @10:05:50.00 Elbow.seek 3 @10:05:50.44 Forearm.seek 3 + 300 counts @10:05:50.71 Forearm.reconfigure LooseForearm @10:05:50.84 Hand.close @10:05:51.68 SE.seek :down @10:05:52.17
Forearm.seek :retract @10:05:53.19 Elbow.seek CC @10:05:53.59 SE.coast @10:05:55.13
Elbow.reconfigure LooseElbow @10:05:55.36 Forearm.reconfigure TightForearm @10:05:55.46 Forearm.seek CC @10:05:57.21 CC.seek :closed @10:06:01.60 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.11 @10:06:01.62 Hand.open @10:06:02.46 Forearm.reconfigure LooseForearm @10:06:02.56 Forearm.seek :retract @10:06:03.64 SC.coast @10:06:03.72 collectSample 1500ml w/bubble point of 55psi @10:06:03.75 Priming 7ml @10:06:03.77 Intake.select :close @10:06:03.79 Exhaust.select :close @10:06:03.82 SS.hold @10:06:04.30 Calibrating pressure and temperature sensors @10:06:04.36 CTV.dial :air @10:06:04.40 SSV.dial :spit,avoiding: :exhaust @10:06:04.46 SS.reconfigure SSfast @10:06:04.59 SS.seek 5ml @10:06:07.49 SSV.dial CBV @10:06:07.56
CBV.dial SSV @10:06:09.05 delay 3.8 seconds @10:06:13.12 SSV.dial :spit @10:06:14.64 SS.seek 0ml @10:06:17.20 SSV.dial CBV @10:06:18.63 CTV.dial :intake @10:06:19.50 SSV.dial CBV @10:06:19.55
CBV.dial SSV @10:06:19.60 Allowing 40s for 7.0ml of remaining 7.0ml @10:06:19.66 Intake.select :open @10:06:21.94 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:06:21.98 SS.reconfigure SSinhale.with maxSpeed: 150 @10:06:22.11 SS.seek 7ml @10:06:25.90 Vacuum too high -- waiting up to 40s @10:06:28.68 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:06:28.72 SS.reconfigure SSinhale.with maxSpeed: 135 @10:06:28.84 SS.seek 7ml @10:06:32.38 Stroke completed -- waiting for pressure to equalize ... @10:06:37.25 Intake.select :close @10:06:37.50 SSV.dial :exhaust @10:06:38.38 Exhaust.select :open @10:06:38.56 SS.reconfigure SSexhale @10:06:38.65 SS.seek 0ml @10:06:41.97 delay 1 second @10:06:42.54
Exhaust.select :close @10:06:42.71 Sampling 1500.0ml @10:06:42.73 Intake.select :close @10:06:42.76 Exhaust.select :close @10:06:42.78 SS.reconfigure SSconfig @10:06:42.87 SS.hold @10:06:43.19 AFV.select :holdingCoil @10:06:43.38
CTV.dial :intake @10:06:43.41 SSV.dial CBV @10:06:43.48
CBV.dial SSV @10:06:43.61 delay 1 second @10:06:44.36
Allowing 130s for 25.0ml of remaining 1500.0ml @10:06:44.39 Intake.select :open @10:06:44.65 ATV.dial :waste @10:06:45.73 AS.reconfigure ASnorm @10:06:45.82 AS.home.seek :empty @10:06:46.68
Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:06:46.71 SS.reconfigure SSinhale.with maxSpeed: 135 @10:06:46.81 SS.seek 25ml @10:06:50.21 Vacuum too high -- waiting up to 40s @10:06:52.63 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:06:52.66 SS.reconfigure SSinhale.with maxSpeed: 122 @10:06:52.76 SS.seek 25ml @10:06:55.83 Vacuum too high -- waiting up to 40s @10:06:58.14 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:06:58.16 SS.reconfigure SSinhale.with maxSpeed: 110 @10:06:58.20 SS.seek 25ml @10:07:01.17 Vacuum too high -- waiting up to 40s @10:07:03.50 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:07:03.52 SS.reconfigure SSinhale.with maxSpeed: 99 @10:07:03.56 SS.seek 25ml @10:07:06.31 Vacuum too high -- waiting up to 40s @10:07:08.31 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:07:08.32 SS.reconfigure SSinhale.with maxSpeed: 89 @10:07:08.37 SS.seek 25ml @10:07:11.27 Vacuum too high -- waiting up to 40s @10:07:13.39 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:07:13.41 SS.reconfigure SSinhale.with maxSpeed: 80 @10:07:13.46 SS.seek 25ml @10:07:16.46 Vacuum too high -- waiting up to 40s @10:07:18.28 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:07:18.30 SS.reconfigure SSinhale.with maxSpeed: 72 @10:07:18.35 SS.seek 25ml @10:07:21.39 Vacuum too high -- waiting up to 40s @10:07:23.46 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:07:23.48 SS.reconfigure SSinhale.with maxSpeed: 65 @10:07:23.53 SS.seek 25ml @10:07:27.70 Vacuum too high -- waiting up to 40s @10:07:29.53 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:07:29.55 SS.reconfigure SSinhale.with maxSpeed: 59 @10:07:29.59 SS.seek 25ml @10:07:31.67 Stroke completed -- waiting for pressure to equalize ... @10:07:35.49 Intake.select :close @10:07:35.65 SSV.dial :exhaust @10:07:36.45 Exhaust.select :open @10:07:36.57 SS.reconfigure SSexhale @10:07:36.61 SS.seek 0ml @10:07:38.41 ATV.dial :waste @10:07:38.43 ARV.dial between carrier and air1 @10:07:39.17 ASV.dial :waste1 @10:07:45.33
Exhaust.select :close @10:07:45.46 SSV.dial CBV @10:07:45.49
CBV.dial SSV @10:07:46.31 Allowing 130s for 25.0ml of remaining 1475.0ml @10:07:46.32 Intake.select :open @10:07:48.50 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:07:48.51 SS.reconfigure SSinhale.with maxSpeed: 59 @10:07:48.56 SS.seek 25ml @10:07:54.27 Vacuum too high -- waiting up to 40s @10:07:55.87 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:07:55.88 SS.reconfigure SSinhale.with maxSpeed: 53 @10:07:55.93 SS.seek 25ml @10:08:00.89 Vacuum too high -- waiting up to 40s @10:08:02.71 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:08:02.73 SS.reconfigure SSinhale.with maxSpeed: 48 @10:08:02.78 SS.seek 25ml @10:08:07.80 Vacuum too high -- waiting up to 40s @10:08:09.61 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:08:09.63 SS.reconfigure SSinhale.with maxSpeed: 43 @10:08:09.68 SS.seek 25ml @10:08:16.16 Vacuum too high -- waiting up to 40s @10:08:17.99 SS.reconfigure SSinhale.with maxSpeed: 39 @10:08:18.03 SS.seek 25ml @10:08:25.38 Vacuum too high -- waiting up to 40s @10:08:27.45 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:08:27.47 SS.reconfigure SSinhale.with maxSpeed: 35 @10:08:27.51 SS.seek 25ml @10:08:36.71 Vacuum too high -- waiting up to 40s @10:08:38.67 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:08:38.69 SS.reconfigure SSinhale.with maxSpeed: 32 @10:08:38.74 SS.seek 25ml @10:08:44.54 Stroke completed -- waiting for pressure to equalize ... @10:08:50.31 Intake.select :close @10:08:50.47 SSV.dial :exhaust @10:08:51.29 Exhaust.select :open @10:08:51.41 SS.reconfigure SSexhale @10:08:51.45 SS.seek 0ml @10:09:00.28 Exhaust.select :close @10:09:00.40 SSV.dial CBV @10:09:00.42
CBV.dial SSV @10:09:01.28 Allowing 130s for 25.0ml of remaining 1450.0ml @10:09:01.29 Intake.select :open @10:09:03.47 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:09:03.48 SS.reconfigure SSinhale.with maxSpeed: 32 @10:09:03.53 SS.seek 25ml @10:09:18.52 Vacuum too high -- waiting up to 40s @10:09:20.60 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:09:20.61 SS.reconfigure SSinhale.with maxSpeed: 29 @10:09:20.66 SS.seek 25ml @10:09:35.64 Vacuum too high -- waiting up to 40s @10:09:37.96 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:09:37.98 SS.reconfigure SSinhale.with maxSpeed: 26 @10:09:38.02 SS.seek 25ml @10:10:00.79 Vacuum too high -- waiting up to 40s @10:10:03.11 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:10:03.13 SS.reconfigure SSinhale.with maxSpeed: 23 @10:10:03.17 SS.seek 25ml @10:10:15.32 Stroke completed -- waiting for pressure to equalize ... @10:10:22.34 Intake.select :close @10:10:22.50 SSV.dial :exhaust @10:10:23.33 Exhaust.select :open @10:10:23.45 SS.reconfigure SSexhale @10:10:23.49 SS.seek 0ml @10:10:32.56 Exhaust.select :close @10:10:32.68 SSV.dial CBV @10:10:32.73
CBV.dial SSV @10:10:33.53 Allowing 130s for 25.0ml of remaining 1425.0ml @10:10:33.54 Intake.select :open @10:10:35.71 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:10:35.73 SS.reconfigure SSinhale.with maxSpeed: 23 @10:10:35.77 SS.seek 25ml @10:11:25.71 Vacuum too high -- waiting up to 40s @10:11:28.04 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:11:28.05 SS.reconfigure SSinhale.with maxSpeed: 21 @10:11:28.10 SS.seek 25ml @10:11:57.57 Stroke completed -- waiting for pressure to equalize ... @10:12:05.76 Intake.select :close @10:12:05.92 SSV.dial :exhaust @10:12:06.73 Exhaust.select :open @10:12:06.85 SS.reconfigure SSexhale @10:12:06.89 SS.seek 0ml @10:12:15.69 Exhaust.select :close @10:12:15.81 SSV.dial CBV @10:12:15.83
CBV.dial SSV @10:12:16.69 Allowing 130s for 25.0ml of remaining 1400.0ml @10:12:16.70 Intake.select :open @10:12:18.88 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:12:18.89 SS.reconfigure SSinhale.with maxSpeed: 21 @10:12:18.94 SS.seek 25ml @10:13:32.27 Vacuum too high -- waiting up to 40s @10:13:35.48 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:13:35.50 SS.reconfigure SSinhale.with maxSpeed: 19 @10:13:35.55 SS.seek 25ml @10:13:47.42 Stroke completed -- waiting for pressure to equalize ... @10:13:54.41 Intake.select :close @10:13:54.57 SSV.dial :exhaust @10:13:55.39 Exhaust.select :open @10:13:55.51 SS.reconfigure SSexhale @10:13:55.55 SS.seek 0ml @10:14:04.81 Exhaust.select :close @10:14:04.93 SSV.dial CBV @10:14:04.95
CBV.dial SSV @10:14:05.78 Allowing 130s for 25.0ml of remaining 1375.0ml @10:14:05.79 Intake.select :open @10:14:07.96 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:14:07.98 SS.reconfigure SSinhale.with maxSpeed: 19 @10:14:08.02 SS.seek 25ml @10:15:40.13 Stroke completed -- waiting for pressure to equalize ... @10:15:48.33 Intake.select :close @10:15:48.49 SSV.dial :exhaust @10:15:49.31 Exhaust.select :open @10:15:49.43 SS.reconfigure SSexhale @10:15:49.47 SS.seek 0ml @10:15:58.64 Exhaust.select :close @10:15:58.76 SSV.dial CBV @10:15:58.78
CBV.dial SSV @10:15:59.62 Allowing 130s for 25.0ml of remaining 1350.0ml @10:15:59.63 Intake.select :open @10:16:01.80 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:16:01.82 SS.reconfigure SSinhale.with maxSpeed: 19 @10:16:01.87 SS.seek 25ml @10:17:33.97 Stroke completed -- waiting for pressure to equalize ... @10:17:42.77 Intake.select :close @10:17:42.93 SSV.dial :exhaust @10:17:43.75 Exhaust.select :open @10:17:43.87 SS.reconfigure SSexhale @10:17:43.91 SS.seek 0ml @10:17:53.12 Exhaust.select :close @10:17:53.24 SSV.dial CBV @10:17:53.26
CBV.dial SSV @10:17:54.09 Allowing 130s for 25.0ml of remaining 1325.0ml @10:17:54.10 Intake.select :open @10:17:56.28 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:17:56.29 SS.reconfigure SSinhale.with maxSpeed: 19 @10:17:56.34 SS.seek 25ml @10:19:28.74 Stroke completed -- waiting for pressure to equalize ... @10:19:38.18 Intake.select :close @10:19:38.34 SSV.dial :exhaust @10:19:39.15 Exhaust.select :open @10:19:39.27 SS.reconfigure SSexhale @10:19:39.31 SS.seek 0ml @10:19:48.75 Exhaust.select :close @10:19:48.87 SSV.dial CBV @10:19:48.89
CBV.dial SSV @10:19:49.72 Allowing 130s for 25.0ml of remaining 1300.0ml @10:19:49.73 Intake.select :open @10:19:51.90 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:19:51.92 SS.reconfigure SSinhale.with maxSpeed: 19 @10:19:51.97 SS.seek 25ml @10:21:24.31 Stroke completed -- waiting for pressure to equalize ... @10:21:34.96 Intake.select :close @10:21:35.11 SSV.dial :exhaust @10:21:35.92 Exhaust.select :open @10:21:36.04 SS.reconfigure SSexhale @10:21:36.09 SS.seek 0ml @10:21:45.00 Exhaust.select :close @10:21:45.12 SSV.dial CBV @10:21:45.14
CBV.dial SSV @10:21:45.98 Allowing 130s for 25.0ml of remaining 1275.0ml @10:21:45.99 Intake.select :open @10:21:48.81 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:21:48.82 SS.reconfigure SSinhale.with maxSpeed: 19 @10:21:48.87 SS.seek 25ml @10:22:56.33 Vacuum too high -- waiting up to 40s @10:22:59.17 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:22:59.19 SS.reconfigure SSinhale.with maxSpeed: 17 @10:22:59.24 SS.seek 25ml @10:23:27.90 Stroke completed -- waiting for pressure to equalize ... @10:23:37.35 Intake.select :close @10:23:37.51 SSV.dial :exhaust @10:23:38.34 Exhaust.select :open @10:23:38.46 SS.reconfigure SSexhale @10:23:38.50 SS.seek 0ml @10:23:47.45 Exhaust.select :close @10:23:47.57 SSV.dial CBV @10:23:47.59
CBV.dial SSV @10:23:48.44 Allowing 130s for 25.0ml of remaining 1250.0ml @10:23:48.45 Intake.select :open @10:23:50.62 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:23:50.64 SS.reconfigure SSinhale.with maxSpeed: 17 @10:23:50.69 SS.seek 25ml @10:25:34.17 Stroke completed -- waiting for pressure to equalize ... @10:25:46.07 Intake.select :close @10:25:46.23 SSV.dial :exhaust @10:25:47.05 Exhaust.select :open @10:25:47.17 SS.reconfigure SSexhale @10:25:47.21 SS.seek 0ml @10:25:56.08 Exhaust.select :close @10:25:56.20 SSV.dial CBV @10:25:56.22
CBV.dial SSV @10:25:57.06 Allowing 130s for 25.0ml of remaining 1225.0ml @10:25:57.07 Intake.select :open @10:25:59.25 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:25:59.26 SS.reconfigure SSinhale.with maxSpeed: 17 @10:25:59.32 SS.seek 25ml @10:27:21.68 Vacuum too high -- waiting up to 40s @10:27:24.76 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:27:24.78 SS.reconfigure SSinhale.with maxSpeed: 15 @10:27:24.83 SS.seek 25ml @10:27:49.30 Stroke completed -- waiting for pressure to equalize ... @10:27:59.98 Intake.select :close @10:28:00.14 SSV.dial :exhaust @10:28:00.95 Exhaust.select :open @10:28:01.07 SS.reconfigure SSexhale @10:28:01.11 SS.seek 0ml @10:28:10.55 Exhaust.select :close @10:28:10.67 SSV.dial CBV @10:28:10.69
CBV.dial SSV @10:28:11.55 Allowing 130s for 25.0ml of remaining 1200.0ml @10:28:11.56 Intake.select :open @10:28:13.73 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:28:13.75 SS.reconfigure SSinhale.with maxSpeed: 15 @10:28:13.80 SS.seek 25ml @10:30:11.04 Stroke completed -- waiting for pressure to equalize ... @10:30:21.57 SS.reconfigure SSconfig @10:30:21.62 SS.hold @10:30:21.75 Intake.select :close @10:30:21.90 ShallowSampler::Clogged in MAIN -- progress too slow after 325.0ml @10:30:21.91 SSV.dial :exhaust @10:30:22.74 Exhaust.select :open @10:30:22.86 SS.reconfigure SSexhale @10:30:22.90 SS.seek 0ml @10:30:32.05 Exhaust.select :close @10:30:32.16 Sampled 325.0ml @10:30:32.18 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:30:32.19 Intake.select :close @10:30:32.26 CSV.dial :waste @10:30:32.32 CS.seek 0ml @10:30:32.43 SSV.dial CBV,avoiding: :exhaust @10:30:32.45
CBV.dial SSV @10:30:32.52 CSV.dial :air @10:30:33.33 CS.seek 10ml @10:30:34.47
CTV.dial :air @10:30:35.27 delay 15 seconds @10:30:50.30 SSV.dial :spit,avoiding: :exhaust @10:30:51.72 CTV.dial CSV,avoiding: :intake @10:30:51.76
SS.seek 0ml @10:30:51.90 SSV.dial CBV,avoiding: :exhaust @10:30:58.06 CBV.dial between SSV and waste2 @10:30:58.48 CTV.dial CSV,avoiding: :air @10:30:58.50
CSV.dial CTV @10:30:59.00 CS.reconfigure CSpressurize @10:30:59.04 CS.seek 0ml @10:31:24.96 BubblePoint: Intake @70.4psia, Outlet @14.7psia @10:31:24.97 CBV.dial SSV @10:31:25.32 SS.reconfigure SSconfig @10:31:25.38 SS.seek 10ml @10:31:30.80 delay 15 seconds @10:31:45.94 Evacuated: Intake @36.6psia, Outlet @1.3psia @10:31:45.95 SSV.dial :spit,avoiding: :exhaust @10:31:47.38 SS.reconfigure SSexhale @10:31:47.43 SS.seek 0ml @10:31:52.40 SS.reconfigure SSfast @10:31:52.45 SS.seek 5ml @10:31:55.14 SSV.dial CBV,avoiding: :exhaust @10:31:55.18
CBV.dial SSV @10:31:56.60 CTV.dial :air,avoiding: :intake @10:31:58.02 CSV.dial CTV @10:31:58.07
SS.seek 10ml @10:31:59.07 CSV.dial :air @10:31:59.56 CS.reconfigure CSfast @10:31:59.61 CS.seek 0ml @10:32:00.71
SSV.dial :spit,avoiding: :exhaust @10:32:00.86 CS.coast @10:32:02.11
SS.reconfigure SSexhale @10:32:02.16 SS.seek 0ml @10:32:07.14 SS.reconfigure SSfast @10:32:07.19 SS.seek 5ml @10:32:09.91 SSV.dial CBV,avoiding: :exhaust @10:32:09.95
CBV.dial SSV @10:32:12.37 SSV.dial :spit,avoiding: :exhaust @10:32:14.42 SS.reconfigure SSconfig @10:32:14.48 SS.seek 0ml @10:32:17.61 SS.coast @10:32:17.65 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:32:17.66 Intake.select :close @10:32:17.74 CSV.dial :waste @10:32:17.88 SSV.dial CBV,avoiding: :exhaust @10:32:17.93
CBV.dial SSV @10:32:18.60 CS.seek 0ml @10:32:18.68 CSV.dial :air @10:32:19.33
CTV.dial :air @10:32:19.35 delay 15 seconds @10:32:19.46 CS.seek 10ml @10:32:34.37
SSV.dial :spit,avoiding: :exhaust @10:32:35.78 CTV.dial CSV,avoiding: :intake @10:32:35.83
SS.reconfigure SSexhale @10:32:35.92 SS.seek 0ml @10:32:35.98 SSV.dial CBV,avoiding: :exhaust @10:32:43.81 CBV.dial between SSV and waste2 @10:32:44.24 CSV.dial between air and CTV @10:32:44.58 CS.seek 8.179ml @10:32:49.85 CTV.dial CSV,avoiding: :air @10:32:49.87
CSV.dial CTV @10:32:50.22 CS.reconfigure CSpressurize @10:32:50.26 CS.seek 0ml @10:33:11.84 CSV.dial :air @10:33:12.34 CS.reconfigure CSfast @10:33:12.40 CS.seek 10ml @10:33:36.46 CSV.dial between air and CTV @10:33:36.82 CS.seek 3.055ml @10:33:53.94 CTV.dial CSV,avoiding: :air @10:33:53.96
CSV.dial CTV @10:33:54.30 CS.reconfigure CSpressurize @10:33:54.34 CS.seek 0ml @10:34:01.83 BubblePoint: Intake @69.9psia, Outlet @14.3psia @10:34:01.84 CBV.dial SSV @10:34:02.18 SS.reconfigure SSconfig @10:34:02.24 SS.seek 10ml @10:34:07.64 delay 15 seconds @10:34:22.78 Evacuated: Intake @39.3psia, Outlet @15.8psia @10:34:22.79 SSV.dial :spit,avoiding: :exhaust @10:34:24.21 SS.reconfigure SSexhale @10:34:24.26 SS.seek 0ml @10:34:29.22 SS.reconfigure SSfast @10:34:29.27 SS.seek 5ml @10:34:31.97 SSV.dial CBV,avoiding: :exhaust @10:34:32.00
CBV.dial SSV @10:34:33.41 CTV.dial :air,avoiding: :intake @10:34:34.82 CSV.dial CTV @10:34:34.86
SS.seek 10ml @10:34:35.86 CSV.dial :air @10:34:36.35 CS.reconfigure CSfast @10:34:36.41 CS.seek 0ml @10:34:37.35
SSV.dial :spit,avoiding: :exhaust @10:34:38.52 CS.coast @10:34:38.78
SS.reconfigure SSexhale @10:34:38.82 SS.seek 0ml @10:34:44.38 SS.reconfigure SSfast @10:34:44.51 SS.seek 5ml @10:34:47.22 SSV.dial CBV,avoiding: :exhaust @10:34:47.25
CBV.dial SSV @10:34:49.68 SSV.dial :spit,avoiding: :exhaust @10:34:51.08 SS.reconfigure SSconfig @10:34:51.13 SS.seek 0ml @10:34:54.30 SS.coast @10:34:54.35 Lysing: Adding 1.5ml lysis @10:34:54.36 CBV.dial between CSV and waste1,avoiding: :DBV @10:34:55.95 CTV.dial CSV @10:34:57.36 CSV.dial CSR @10:34:58.20 Pulling 0.999ml of CSR.lysis @10:34:58.21 CSR.select :lysis @10:34:58.33 CS.reconfigure CSconfig @10:34:58.37 CS.seek 1ml @10:35:01.93 delay 5 seconds @10:35:06.96 CSR.select :air @10:35:07.13 CS.seek 6ml @10:35:20.93 delay 2 seconds @10:35:22.95 CSV.dial :waste1 @10:35:24.45 CS.reconfigure CSfast @10:35:24.51 CS.seek :empty @10:35:39.31 CSV.dial CSR @10:35:40.76 Pulling 1.5ml of CSR.lysis @10:35:40.77 CSR.select :lysis @10:35:40.89 CS.reconfigure CSconfig @10:35:40.93 CS.seek 1.5ml @10:35:45.84 delay 5 seconds @10:35:50.87 CSR.select :air @10:35:51.05 CS.seek 4.5ml @10:35:59.69 delay 2 seconds @10:36:01.71 CSV.dial CTV @10:36:02.21 CS.seek 1.275ml @10:36:11.48 Heating sh1 puck from tube 3 in Collection Clamp to 85C for 5:00 @10:36:11.50 CH.seek 85C,8:00 @10:39:21.89 CH.hold 5:00,85C @10:44:22.07 Collection Puck Heater at 85.1C after lysing @10:44:22.09 CS.seek 0ml @10:44:26.35 CTV.dial between spare and air,avoiding: :intake @10:44:27.48 CSV.dial :air @10:44:27.97 CS.seek 5ml @10:44:41.76 CSV.dial CTV @10:44:42.26 delay 2 seconds @10:44:44.28 CSV.dial :air @10:44:44.77 delay 2 seconds @10:44:46.79 CSV.dial CTV @10:44:47.40 delay 2 seconds @10:44:49.43 CSV.dial :air @10:44:49.90 delay 2 seconds @10:44:51.93 CS.seek 0ml @10:45:05.73 Recovering Lysate into Collection syringe @10:45:05.74 CSV.dial CBV @10:45:06.82 CBV.dial CSV @10:45:07.23 CS.seek 5ml @10:45:21.07 delay 5 seconds @10:45:26.10 CTV.dial :air @10:45:26.67 CS.seek 7ml @10:45:32.79 delay 5 seconds @10:45:37.81 CSV.dial :air2 @10:45:38.28 CS.seek 10ml @10:45:47.01 CSV.dial between air and CTV,avoiding: CBV @10:45:48.23 Lysing Complete @10:45:48.31 Storing sh1 puck in Collection Clamp to tube 2 @10:45:48.50 SC.reconfigure SCtight @10:45:48.55 SC.seek 2 @10:45:50.71 SC.reconfigure SCloose @10:45:50.93 Elbow.reconfigure TightElbow @10:45:50.98 Elbow.seek 2 @10:45:52.95 Forearm.reconfigure TightForearm @10:45:53.00 Forearm.seek 2 + 700 counts @10:45:54.00 SE.reconfigure SEpick @10:45:54.08 SE.seek beamBreak @10:45:56.37 SE.configure SEconfig @10:45:56.53 Elbow.reconfigure LimpElbow @10:45:56.59 Forearm.reconfigure LooseForearm @10:45:56.63 Forearm.seek 2 @10:45:56.96 Elbow.hold @10:45:57.03 Elbow.reconfigure TightElbow @10:45:57.14 SE.seek 8.08 Pucks below Top Plate @10:45:57.58 Forearm.force -20 @10:45:57.62 Forearm.seek 2 + 320 counts @10:45:57.96 Forearm.reconfigure TightForearm @10:45:58.57 Forearm.seek 2 + 700 counts @10:45:59.06 SE.seek 9.08 Pucks below Top Plate @10:45:59.68 Forearm.reconfigure LooseForearm @10:45:59.73 Forearm.seek :retract @10:46:00.69 Elbow.seek CC @10:46:02.39 Elbow.reconfigure LooseElbow @10:46:02.50 Forearm.seek CC @10:46:03.57 Hand.close @10:46:04.36 CC.seek :open @10:46:08.86 Forearm.seek :retract @10:46:09.90 Elbow.reconfigure TightElbow @10:46:09.95 Elbow.seek 2 @10:46:11.81 Elbow.reconfigure LimpElbow @10:46:11.87 Forearm.seek 2 @10:46:12.90 Elbow.hold @10:46:12.97 Elbow.reconfigure TightElbow @10:46:13.02 Hand.open @10:46:13.88 Forearm.force -20 @10:46:13.93 Forearm.seek 2 + 349 counts @10:46:14.25 Forearm.reconfigure TightForearm @10:46:14.30 Forearm.seek 2 + 700 counts @10:46:14.67 SE.seek :down @10:46:15.82
Forearm.reconfigure LooseForearm @10:46:15.87 Forearm.seek :retract @10:46:16.54 SE.coast @10:46:16.83
SC.coast @10:46:16.87 Tranferring lysate to PRV @10:46:16.88 CSV.dial :air @10:46:17.21 CS.seek 10ml @10:46:17.28 CSV.dial between air and CTV @10:46:17.62 AIV.dial :bypass @10:46:17.71 Beginning samplePassC @10:46:17.73 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:46:17.80 Hand.open @10:46:18.13 Elbow.seek FlushPuck::Garage @10:46:20.18 Elbow.reconfigure LooseElbow @10:46:20.29 Forearm.seek :garage @10:46:21.29 Hand.close @10:46:22.06 Forearm.seek :retract @10:46:23.08 Elbow.reconfigure TightElbow @10:46:23.13 Elbow.seek CC @10:46:26.07 Elbow.reconfigure LooseElbow @10:46:26.18 Forearm.reconfigure TightForearm @10:46:26.23 Forearm.seek CC @10:46:28.21 CC.seek :closed @10:46:32.53 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.03 @10:46:32.62 Hand.open @10:46:33.31 Forearm.reconfigure LooseForearm @10:46:33.36 Forearm.seek :retract @10:46:34.35 AWV.select :air @10:46:34.36 AIV.dial :bypass @10:46:34.37 PSV.dial PSR @10:46:35.82 Pulling 1.5ml of PSR.flush @10:46:35.83 PSR.select :flush @10:46:35.96 PS.seek 1.5ml @10:46:52.08 delay 5 seconds @10:46:57.11 PSV.dial :waste1 @10:46:57.73 PS.seek 0ml @10:47:13.74 PSV.dial PSR @10:47:14.21 PSR.select :air @10:47:14.34 PS.seek 2.5ml @10:47:40.56 PRV.dial CBV @10:47:40.58
CBV.dial PRV @10:47:41.45 PSV.dial :mixloop @10:47:42.52 CTV.dial :air,avoiding: :intake @10:47:42.53 PTV.dial :PRVmixing @10:47:42.63 PS.seek 0ml @10:48:08.71 PSV.dial :air @10:48:09.19 PS.seek 2.5ml @10:48:35.30 PSV.dial :mixloop @10:48:35.78 PS.seek 0ml @10:49:01.90 PSV.dial :air @10:49:02.39 PS.seek 2.5ml @10:49:28.55 PSV.dial :mixloop @10:49:29.03 PS.seek 0ml @10:49:55.15 Garaging FlushPuck in Collection Clamp @10:49:55.23 Elbow.reconfigure TightElbow @10:49:55.28 Elbow.seek CC @10:49:55.52 Elbow.reconfigure LooseElbow @10:49:55.63 Forearm.seek CC @10:49:56.68 Hand.close @10:49:57.25 CC.seek :open @10:50:01.61 Forearm.seek :retract @10:50:02.64 Elbow.reconfigure TightElbow @10:50:02.69 Elbow.seek :garage @10:50:05.85 Elbow.reconfigure LooseElbow @10:50:05.96 Forearm.reconfigure TightForearm @10:50:06.01 Forearm.seek :garage @10:50:07.18 Hand.open @10:50:07.82 Forearm.reconfigure LooseForearm @10:50:07.87 Forearm.seek :retract @10:50:08.92 Connecting PS to CS to transfer sample @10:50:08.93 CBV.dial AIV @10:50:08.94 AIV.dial :bypass @10:50:08.95 PSV.dial :mixloop @10:50:08.97 PTV.dial :PRVmixing @10:50:08.98 PRV.dial AIV @10:50:08.99 CTV.dial :air,avoiding: :intake @10:50:09.01 Loading lyfil puck from tube 3 into Collection Clamp @10:50:09.92 SC.reconfigure SCtight @10:50:09.97 SC.seek 3 @10:50:12.14 SC.reconfigure SCloose @10:50:12.38 Elbow.reconfigure TightElbow @10:50:12.43 Elbow.seek 3 @10:50:14.49 Forearm.reconfigure TightForearm @10:50:14.54 Forearm.seek 3 + 700 counts @10:50:15.61 SE.reconfigure SEpick @10:50:15.69 SE.seek beamBreak @10:50:17.64 SE.configure SEconfig @10:50:17.81 Elbow.seek 3 @10:50:18.08 Forearm.seek 3 + 300 counts @10:50:18.29 Forearm.reconfigure LooseForearm @10:50:18.34 Hand.close @10:50:19.02 SE.seek :down @10:50:19.25
Forearm.seek :retract @10:50:20.18 Elbow.seek CC @10:50:20.94 SE.coast @10:50:21.91
Elbow.reconfigure LooseElbow @10:50:22.01 Forearm.reconfigure TightForearm @10:50:22.06 Forearm.seek CC @10:50:23.42 CC.seek :closed @10:50:27.67 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.11 @10:50:27.68 Hand.open @10:50:28.32 Forearm.reconfigure LooseForearm @10:50:28.37 Forearm.seek :retract @10:50:29.37 SC.coast @10:50:29.40 CTV.dial CSV,avoiding: :intake @10:50:30.83 CSV.dial CTV @10:50:31.16 CS.seek 5ml @10:50:44.92 delay 1:20 @10:52:04.96 CS.seek 2.5ml @10:52:12.33 delay 40 seconds @10:52:52.37 CS.seek 0ml @10:52:59.80 delay 1:00 @10:53:59.83 PSV.dial :air @10:54:00.32 PS.seek 2.5ml @10:54:26.43 PSV.dial :mixloop @10:54:26.90 delay 3 seconds @10:54:29.92 PSV.dial :air @10:54:30.39 delay 2 seconds @10:54:32.41 PSV.dial :mixloop @10:54:32.89 delay 3 seconds @10:54:35.91 PSV.dial :waste1 @10:54:37.29 PS.seek :empty @10:55:03.37 CBV.dial :waste1 @10:55:03.88 delay 5 seconds @10:55:08.91 CBV.dial AIV @10:55:09.41 delay 10 seconds @10:55:19.44 CBV.dial :waste1 @10:55:19.94 delay 10 seconds @10:55:29.97 CBV.dial AIV @10:55:30.47 delay 10 seconds @10:55:40.49 CTV.dial CSV,avoiding: :intake @10:55:40.50 CBV.dial between AIV and waste1 @10:55:41.48 CSV.dial :air @10:55:42.03 CS.seek 10ml @10:56:08.52 CSV.dial CTV @10:56:09.01 CS.seek 0ml @10:56:35.71 CBV.dial :waste1 @10:56:36.04 delay 10 seconds @10:56:46.07 CTV.dial :air,avoiding: :intake @10:56:47.56 Storing lyfil puck in Collection Clamp to tube 2 @10:56:47.75 SC.reconfigure SCtight @10:56:47.80 SC.seek 2 @10:56:49.99 SC.reconfigure SCloose @10:56:50.21 Elbow.reconfigure TightElbow @10:56:50.26 Elbow.seek 2 @10:56:52.15 Forearm.reconfigure TightForearm @10:56:52.21 Forearm.seek 2 + 700 counts @10:56:53.34 SE.reconfigure SEpick @10:56:53.42 SE.seek beamBreak @10:56:55.77 SE.configure SEconfig @10:56:55.93 Elbow.reconfigure LimpElbow @10:56:55.99 Forearm.reconfigure LooseForearm @10:56:56.03 Forearm.seek 2 @10:56:56.29 Elbow.hold @10:56:56.36 Elbow.reconfigure TightElbow @10:56:56.47 SE.seek 9.06 Pucks below Top Plate @10:56:56.96 Forearm.force -20 @10:56:57.00 Forearm.seek 2 + 345 counts @10:56:57.35 Forearm.reconfigure TightForearm @10:56:57.39 Forearm.seek 2 + 700 counts @10:56:57.77 SE.seek 10.06 Pucks below Top Plate @10:56:58.38 Forearm.reconfigure LooseForearm @10:56:58.43 Forearm.seek :retract @10:56:59.37 Elbow.seek CC @10:57:00.99 Elbow.reconfigure LooseElbow @10:57:01.10 Forearm.seek CC @10:57:02.16 Hand.close @10:57:02.79 CC.seek :open @10:57:07.31 Forearm.seek :retract @10:57:08.32 Elbow.reconfigure TightElbow @10:57:08.37 Elbow.seek 2 @10:57:10.25 Elbow.reconfigure LimpElbow @10:57:10.30 Forearm.seek 2 @10:57:11.29 Elbow.hold @10:57:11.36 Elbow.reconfigure TightElbow @10:57:11.41 Hand.open @10:57:12.14 Forearm.force -20 @10:57:12.20 Forearm.seek 2 + 344 counts @10:57:12.51 Forearm.reconfigure TightForearm @10:57:12.56 Forearm.seek 2 + 700 counts @10:57:13.06 SE.seek :down @10:57:14.21
Forearm.reconfigure LooseForearm @10:57:14.26 Forearm.seek :retract @10:57:15.53 SE.coast @10:57:15.58
SC.coast @10:57:15.61 PSV.dial :air @10:57:16.76 PS.seek 0.499ml @10:57:22.69 delay 3 seconds @10:57:25.71 PSV.dial :mixloop @10:57:26.17 PRV.dial :air @10:57:26.66 delay 3 seconds @10:57:29.69 PRV.dial CBV @10:57:30.19 CBV.dial AIV @10:57:30.69 PRV.dial between CBV and air @10:57:31.05 PRV.dial :air @10:57:31.44 PS.reconfigure PSslow @10:57:31.49 PS.seek 0.599ml @10:57:34.06 PRV.dial :waste2 @10:57:34.83 PS.reconfigure PSconfig @10:57:34.88 PS.seek :empty @10:57:41.84 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:57:41.92 Hand.open @10:57:42.26 Elbow.seek FlushPuck::Garage @10:57:44.12 Elbow.reconfigure LooseElbow @10:57:44.23 Forearm.seek :garage @10:57:45.24 Hand.close @10:57:46.01 Forearm.seek :retract @10:57:47.05 Elbow.reconfigure TightElbow @10:57:47.09 Elbow.seek CC @10:57:50.05 Elbow.reconfigure LooseElbow @10:57:50.16 Forearm.reconfigure TightForearm @10:57:50.21 Forearm.seek CC @10:57:51.55 CC.seek :closed @10:57:55.80 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.98 @10:57:55.88 Hand.open @10:57:56.58 Forearm.reconfigure LooseForearm @10:57:56.63 Forearm.seek :retract @10:57:57.75 Clearing PML for modified lysate @10:57:57.76 CTV.dial :air,avoiding: :intake @10:57:57.77 PRV.dial :waste2 @10:57:57.78 PTV.dial :PRV,avoiding: :puck @10:57:58.58 delay 2 seconds @10:58:00.60 PTV.dial :PRVmixing,avoiding: :puck @10:58:01.39 delay 2 seconds @10:58:03.41 PSV.dial :PML @10:58:03.42 delay 2 seconds @10:58:05.45 PRV.dial :air @10:58:06.23 delay 2 seconds @10:58:08.31 PS.seek 1.499ml @10:58:24.31 delay 5 seconds @10:58:29.34 PS.seek 2.5ml @10:58:40.25 PSV.dial :air @10:58:41.38 PS.seek 0ml @10:59:07.53 mixing lysate with speDil @10:59:07.54 PSV.dial :PML @10:59:08.02 CBV.dial :PRV @10:59:08.03 PRV.dial :air @10:59:08.05 delay 2 seconds @10:59:10.07 PRV.dial :speDil @10:59:10.56 delay 2 seconds @10:59:12.65 PS.reconfigure PSslow1 @10:59:12.70 PS.seek 0.225ml @10:59:36.16 delay 5 seconds @10:59:41.19 PRV.dial CBV @10:59:41.99 delay 2 seconds @10:59:44.07 PS.seek 0.475ml @11:00:09.75 delay 5 seconds @11:00:14.78 PRV.dial :air @11:00:15.27 delay 2 seconds @11:00:17.30 PS.seek 0.965ml @11:01:07.19 delay 10 seconds @11:01:17.22 PRV.dial CBV @11:01:17.71 CTV.dial :air,avoiding: :intake @11:01:17.72 PSV.dial :air @11:01:18.21 PS.reconfigure PSconfig @11:01:18.26 PS.seek 2.5ml @11:01:34.60 delay 3 seconds @11:01:37.62 PSV.dial PTV @11:01:38.41 PTV.dial :PRV,avoiding: :puck @11:01:39.22 PS.seek 0ml @11:02:05.32 PSV.dial :air @11:02:06.11 PS.seek 2.5ml @11:02:32.25 delay 3 seconds @11:02:35.27 PSV.dial PTV @11:02:36.13 PTV.dial :PRV,avoiding: :puck @11:02:36.15 PS.seek 0ml @11:03:02.35 Pushing modified lysate into AIV Injection Loop @11:03:02.36 CBV.dial AIV @11:03:02.37 AIV.dial :bypass @11:03:02.39 PRV.dial CBV @11:03:02.40 PSV.dial :air @11:03:03.19 PS.seek 2.5ml @11:03:29.26 delay 3 seconds @11:03:32.28 PSV.dial :PML @11:03:32.76 PTV.dial :PRVmixing,avoiding: :puck @11:03:33.62 PS.reconfigure PSslow1 @11:03:33.67 PS.seek 1.74ml @11:04:51.22 delay 5 seconds @11:04:56.25 AIV.dial :sample @11:04:56.86 AWV.select :waste @11:04:57.03 PS.reconfigure PSslow2 @11:04:57.08 PS.seek 1.22ml @11:06:44.20 delay 15 seconds @11:06:59.23 AIV.dial :bypass @11:06:59.83 PS.reconfigure PSconfig @11:06:59.88 PS.seek 0ml @11:07:13.13 PSV.dial :air @11:07:13.61 PS.seek 2.5ml @11:07:39.76 PSV.dial :PML @11:07:40.24 PS.seek 0ml @11:08:06.45 AIV.dial :coilToMFB @11:08:06.46 AWV.select :air @11:08:06.63 Extracting Nucleic Acid from AIV @11:08:06.66
Beginning samplePassD @11:08:06.70 SPE.hold 2:46:40,55C @11:08:06.79 ASV.dial :SPEdelivery @11:08:06.87
AWV.select :air @11:08:06.89 AIV.dial :bypass @11:08:06.90 PSV.dial PSR @11:08:07.22 delay 20 seconds @11:08:08.10
Pulling 1.5ml of PSR.killMFB @11:08:08.11 PSR.select :killMFB @11:08:08.24 PS.seek 1.5ml @11:08:24.32 delay 5 seconds @11:08:27.25 primeLysateINT @11:08:27.26 AWV.select :air @11:08:27.27 AIV.dial :coilToMFB @11:08:27.29 ATV.dial AIV @11:08:29.07 AS.reconfigure ASslow1 @11:08:29.77 AS.seek 0.299ml @11:08:29.81
PSV.dial :waste1 @11:08:30.32 PS.seek 0ml @11:08:46.32 PSV.dial PSR @11:08:46.81 PSR.select :air @11:08:46.94 PS.seek 2.5ml @11:09:13.04 PRV.dial CBV @11:09:13.07
CBV.dial PRV @11:09:13.09 PSV.dial :mixloop @11:09:14.21 CTV.dial :air,avoiding: :intake @11:09:14.22 PTV.dial :PRVmixing @11:09:14.25 PS.seek 0ml @11:09:30.44 delay 10 seconds @11:09:40.35
PSV.dial PSR @11:09:40.46 ATV.dial :air2 @11:09:41.32 AS.seek 0.329ml @11:09:41.53
Pulling 1.5ml of PSR.flush @11:09:41.54 PSR.select :flush @11:09:41.66 PS.seek 1.5ml @11:09:47.63 ATV.dial :waste2 @11:09:48.20 AS.reconfigure ASnorm @11:09:48.25 AS.seek :empty @11:09:57.73
delay 5 seconds @11:10:02.76 PSV.dial :waste1 @11:10:03.27 PS.seek 0ml @11:10:05.27 Lysate primed! @11:10:05.28 AFV.select :holdingCoil @11:10:05.29 ATV.dial :air2 @11:10:05.93 AS.reconfigure ASslow1 @11:10:05.98 AS.seek 0.069ml @11:10:19.29
PSV.dial PSR @11:10:19.90 PSR.select :air @11:10:20.03 PS.seek 2.5ml @11:10:20.35 delay 3 seconds @11:10:23.37 ATV.dial :AIV @11:10:24.24 AS.seek 0.469ml @11:10:46.14 PRV.dial CBV @11:10:46.17
CBV.dial PRV @11:10:46.19 PSV.dial :mixloop @11:10:47.31 CTV.dial :air,avoiding: :intake @11:10:47.32 PTV.dial :PRVmixing @11:10:47.34 PS.seek 0ml @11:11:13.54 PSV.dial PSR @11:11:14.70 Pulling 1.5ml of PSR.flush @11:11:14.71 PSR.select :flush @11:11:14.84 PS.seek 1.5ml @11:11:30.79 delay 5 seconds @11:11:35.82 PSV.dial :waste1 @11:11:36.34 PS.seek 0ml @11:11:44.92 delay 5 seconds @11:11:50.01 Solid Phase Extraction Heater at 45.9C @11:11:50.02 ATV.dial ASV @11:11:50.42 ASV.dial :SPEdelivery @11:11:50.50 AS.seek 0.041ml @11:11:52.29
PSV.dial PSR @11:11:52.78 PSR.select :air @11:11:52.91 PS.seek 2.5ml @11:12:19.14 PRV.dial CBV @11:12:19.17
CBV.dial PRV @11:12:19.18 PSV.dial :mixloop @11:12:20.29 CTV.dial :air,avoiding: :intake @11:12:20.30 PTV.dial :PRVmixing @11:12:20.32 PS.seek 0ml @11:12:46.45 PSV.dial PSR @11:12:47.60 Pulling 1.5ml of PSR.flush @11:12:47.61 PSR.select :flush @11:12:47.74 PS.seek 1.5ml @11:13:03.76 delay 5 seconds @11:13:08.79 PSV.dial :waste1 @11:13:09.29 PS.seek 0ml @11:13:16.83 delay 10 seconds @11:13:25.26
PSV.dial PSR @11:13:25.75 PSR.select :air @11:13:25.88 PS.seek 2.5ml @11:13:26.85 rinseLysateLineINT @11:13:26.86 AFV.select :carrierSolution @11:13:26.97 delay 2 seconds @11:13:28.99 AS.reconfigure ASconfig @11:13:29.04 AS.seek 1ml @11:13:52.70 PRV.dial CBV @11:13:52.73
CBV.dial PRV @11:13:52.75 PSV.dial :mixloop @11:13:53.83 CTV.dial :air,avoiding: :intake @11:13:53.84 PTV.dial :PRVmixing @11:13:53.86 PS.reconfigure PSslow @11:13:53.91 PS.seek 1.2ml @11:14:20.70 PSV.dial :waste1 @11:14:22.11 PS.reconfigure PSconfig @11:14:22.16 PS.seek 0ml @11:14:24.41 delay 1 second @11:14:25.43 AFV.select :holdingCoil @11:14:25.53 delay 1 second @11:14:26.56 ATV.dial AIV @11:14:27.10 AIV.dial :coilToMFB @11:14:27.11 AWV.select :waste @11:14:27.23 AS.reconfigure ASslow0 @11:14:27.27 AS.seek 0ml @11:14:35.11
PSV.dial :air @11:14:36.34 PS.seek 2.5ml @11:15:02.48 PSV.dial :mixloop @11:15:02.98 PS.reconfigure PSslow @11:15:03.02 PS.seek 0ml @11:15:54.08 PSV.dial :air @11:15:54.56 PS.reconfigure PSconfig @11:15:54.61 PS.seek 2.5ml @11:16:08.15 ATV.dial :air2 @11:16:08.83 AS.reconfigure ASslow1 @11:16:08.88 AS.seek 0.05ml @11:16:19.24 ATV.dial :AIV @11:16:20.04 AS.seek 0.01ml @11:16:20.74
PSV.dial :mixloop @11:16:21.23 PS.reconfigure PSslow @11:16:21.27 PS.seek 0ml @11:16:28.38 ATV.dial :waste @11:16:29.97 AS.seek :empty @11:16:32.40 ethanol wash @11:16:32.41 ATV.dial :air2 @11:16:33.64 AS.reconfigure ASslow2 @11:16:33.69 AS.seek 0.009ml @11:16:43.97 delay 3 seconds @11:16:47.00 Pulling 0.4ml of Ethanol Wash @11:16:47.01 ATV.dial :ethanol @11:16:47.83 AS.reconfigure ASslow1 @11:16:47.88 AS.seek 0.409ml @11:17:12.46
Cleaning Collection Syringe @11:17:12.47 CSV.dial CSR @11:17:13.01 Pulling 4ml of CSR.flush @11:17:13.02 CSR.select :flush @11:17:13.15 CS.seek 4ml @11:17:24.39 delay 5 seconds @11:17:29.42 CSR.select :air @11:17:29.54 CS.seek 10ml @11:17:45.80 CSV.dial CTV @11:17:45.84
CTV.dial CSV @11:17:47.33 CBV.dial :waste1 @11:17:47.85 CS.seek 0ml @11:18:08.59 delay 3 seconds @11:18:11.62 ATV.dial :air1 @11:18:12.01 delay 3 seconds @11:18:14.61
CSV.dial CSR @11:18:15.10 AS.seek 0.434ml @11:18:15.22
Pulling 4ml of CSR.flush @11:18:15.23 CSR.select :flush @11:18:15.36 CS.seek 4ml @11:18:20.38 delay 3 seconds @11:18:23.41 ATV.dial :ethanol @11:18:24.00 AS.seek 0.419ml @11:18:26.51
delay 5 seconds @11:18:27.30 delay 3 seconds @11:18:30.32 ATV.dial ASV @11:18:31.59
CSR.select :air @11:18:31.67 AS.seek 0.092ml @11:18:31.73
CS.seek 10ml @11:18:48.08 CSV.dial CTV @11:18:48.74
CTV.dial CSV @11:18:49.28 CBV.dial :waste1 @11:18:49.30 CS.seek 0ml @11:19:15.90 Garaging FlushPuck in Collection Clamp @11:19:15.98 Elbow.reconfigure TightElbow @11:19:16.03 Elbow.seek CC @11:19:16.29 Elbow.reconfigure LooseElbow @11:19:16.39 Forearm.seek CC @11:19:17.39 Hand.close @11:19:18.06 CC.seek :open @11:19:22.43 Forearm.seek :retract @11:19:23.41 Elbow.reconfigure TightElbow @11:19:23.46 Elbow.seek :garage @11:19:26.51 Elbow.reconfigure LooseElbow @11:19:26.61 Forearm.reconfigure TightForearm @11:19:26.66 Forearm.seek :garage @11:19:27.81 Hand.open @11:19:28.49 Forearm.reconfigure LooseForearm @11:19:28.54 Forearm.seek :retract @11:19:29.53 Whole Cell Archive sampling 1500ml @11:19:29.55 Loading wcr puck from tube 3 into Collection Clamp @11:19:29.82 SC.reconfigure SCtight @11:19:29.87 SC.seek 3 @11:19:31.99 SC.reconfigure SCloose @11:19:32.22 Elbow.reconfigure TightElbow @11:19:32.27 Elbow.seek 3 @11:19:34.33 Forearm.reconfigure TightForearm @11:19:34.38 Forearm.seek 3 + 700 counts @11:19:35.44 SE.reconfigure SEpick @11:19:35.52 SE.seek beamBreak @11:19:37.72 delay 3 seconds @11:19:37.81 SE.configure SEconfig @11:19:37.99 Elbow.seek 3 @11:19:38.20 Forearm.seek 3 + 300 counts @11:19:38.39 Forearm.reconfigure LooseForearm @11:19:38.44 Hand.close @11:19:39.07 SE.seek :down @11:19:39.32 Forearm.seek :retract @11:19:40.31 Elbow.seek CC @11:19:40.76 ATV.dial :waste2 @11:19:40.90 SE.coast @11:19:41.49 AS.reconfigure ASconfig @11:19:41.54 AS.seek :empty @11:19:41.96 Elbow.reconfigure LooseElbow @11:19:42.07 Forearm.reconfigure TightForearm @11:19:42.12 Forearm.seek CC @11:19:44.15 CC.seek :closed @11:19:47.19 ATV.dial :air2 @11:19:47.62 AS.reconfigure ASslow1 @11:19:47.70 AS.seek 1ml @11:19:48.41 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.13 @11:19:48.42 Hand.open @11:19:49.12 Forearm.reconfigure LooseForearm @11:19:49.17 Forearm.seek :retract @11:19:50.22 SC.coast @11:19:50.26 collectSample 1500ml w/bubble point of 55psi @11:19:50.28 Priming 7ml @11:19:50.29 Intake.select :close @11:19:50.30 Exhaust.select :close @11:19:50.31 SS.hold @11:19:50.54 Calibrating pressure sensors @11:19:50.55 CTV.dial :air @11:19:51.99 SSV.dial :spit,avoiding: :exhaust @11:19:52.01 SS.reconfigure SSfast @11:19:52.07 SS.seek 5ml @11:19:54.69 SSV.dial CBV @11:19:54.72 CBV.dial SSV @11:19:56.27 delay 3.8 seconds @11:20:00.22 SSV.dial :spit @11:20:01.65 SS.seek 0ml @11:20:04.48 SSV.dial CBV @11:20:05.89 CTV.dial :intake @11:20:06.70 SSV.dial CBV @11:20:06.73 CBV.dial SSV @11:20:06.75 Allowing 40s for 7.0ml of remaining 7.0ml @11:20:06.76 Intake.select :open @11:20:08.94 Intake @32.3psia, vacuum from 20.0 to 24.0psi @11:20:08.96 SS.reconfigure SSinhale.with maxSpeed: 150 @11:20:09.00 SS.seek 7ml @11:20:12.29 Vacuum too high -- waiting up to 40s @11:20:15.41 Intake @32.1psia, vacuum from 20.0 to 24.0psi @11:20:15.43 SS.reconfigure SSinhale.with maxSpeed: 135 @11:20:15.48 SS.seek 7ml @11:20:18.47 Stroke completed -- waiting for pressure to equalize ... @11:20:25.53 Intake.select :close @11:20:25.69 SSV.dial :exhaust @11:20:26.52 Exhaust.select :open @11:20:26.64 SS.reconfigure SSexhale @11:20:26.69 SS.seek 0ml @11:20:30.61 Exhaust.select :close @11:20:30.72 Sampling 1500.0ml @11:20:30.74 Intake.select :close @11:20:30.75 Exhaust.select :close @11:20:30.76 SS.reconfigure SSconfig @11:20:30.81 SS.hold @11:20:31.03 CTV.dial :intake @11:20:31.06 SSV.dial CBV @11:20:31.08 CBV.dial SSV @11:20:31.94 Allowing 130s for 25.0ml of remaining 1500.0ml @11:20:31.96 Intake.select :open @11:20:34.14 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:20:34.16 SS.reconfigure SSinhale.with maxSpeed: 135 @11:20:34.20 SS.seek 25ml @11:20:38.01 Vacuum too high -- waiting up to 40s @11:20:40.66 Intake @32.0psia, vacuum from 20.0 to 24.0psi @11:20:40.68 SS.reconfigure SSinhale.with maxSpeed: 122 @11:20:40.72 SS.seek 25ml @11:20:43.66 Vacuum too high -- waiting up to 40s @11:20:46.05 Intake @32.1psia, vacuum from 20.0 to 24.0psi @11:20:46.07 SS.reconfigure SSinhale.with maxSpeed: 110 @11:20:46.12 SS.seek 25ml @11:20:48.82 Vacuum too high -- waiting up to 40s @11:20:50.90 Intake @32.1psia, vacuum from 20.0 to 24.0psi @11:20:50.92 SS.reconfigure SSinhale.with maxSpeed: 99 @11:20:50.97 SS.seek 25ml @11:20:53.76 Vacuum too high -- waiting up to 40s @11:20:56.56 Intake @32.3psia, vacuum from 20.0 to 24.0psi @11:20:56.58 SS.reconfigure SSinhale.with maxSpeed: 89 @11:20:56.62 SS.seek 25ml @11:20:59.85 Vacuum too high -- waiting up to 40s @11:21:01.73 Intake @32.0psia, vacuum from 20.0 to 24.0psi @11:21:01.75 SS.reconfigure SSinhale.with maxSpeed: 80 @11:21:01.80 SS.seek 25ml @11:21:04.29 Vacuum too high -- waiting up to 40s @11:21:06.18 SS.reconfigure SSinhale.with maxSpeed: 72 @11:21:06.23 SS.seek 25ml @11:21:09.21 Vacuum too high -- waiting up to 40s @11:21:11.08 Intake @32.0psia, vacuum from 20.0 to 24.0psi @11:21:11.10 SS.reconfigure SSinhale.with maxSpeed: 65 @11:21:11.15 SS.seek 25ml @11:21:14.15 Vacuum too high -- waiting up to 40s @11:21:16.04 Intake @32.1psia, vacuum from 20.0 to 24.0psi @11:21:16.06 SS.reconfigure SSinhale.with maxSpeed: 59 @11:21:16.11 SS.seek 25ml @11:21:19.50 Vacuum too high -- waiting up to 40s @11:21:21.38 SS.reconfigure SSinhale.with maxSpeed: 53 @11:21:21.43 SS.seek 25ml @11:21:22.75 Stroke completed -- waiting for pressure to equalize ... @11:21:25.98 Intake.select :close @11:21:26.83 SSV.dial :exhaust @11:21:27.67 Exhaust.select :open @11:21:27.79 SS.reconfigure SSexhale @11:21:27.84 SS.seek 0ml @11:21:36.84 Exhaust.select :close @11:21:36.95 SSV.dial CBV @11:21:36.99 CBV.dial SSV @11:21:37.83 Allowing 130s for 25.0ml of remaining 1475.0ml @11:21:37.85 Intake.select :open @11:21:40.03 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:21:40.05 SS.reconfigure SSinhale.with maxSpeed: 53 @11:21:40.09 SS.seek 25ml @11:21:47.12 Vacuum too high -- waiting up to 40s @11:21:48.82 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:21:48.84 SS.reconfigure SSinhale.with maxSpeed: 48 @11:21:48.88 SS.seek 25ml @11:21:55.57 Vacuum too high -- waiting up to 40s @11:21:57.18 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:21:57.20 SS.reconfigure SSinhale.with maxSpeed: 43 @11:21:57.25 SS.seek 25ml @11:22:06.79 Vacuum too high -- waiting up to 40s @11:22:08.41 SS.reconfigure SSinhale.with maxSpeed: 39 @11:22:08.46 SS.seek 25ml @11:22:25.93 Vacuum too high -- waiting up to 40s @11:22:27.80 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:22:27.82 SS.reconfigure SSinhale.with maxSpeed: 35 @11:22:27.87 SS.seek 25ml @11:22:29.61 Stroke completed -- waiting for pressure to equalize ... @11:22:32.85 Intake.select :close @11:22:33.01 SSV.dial :exhaust @11:22:33.83 Exhaust.select :open @11:22:33.95 SS.reconfigure SSexhale @11:22:34.00 SS.seek 0ml @11:22:42.84 Exhaust.select :close @11:22:42.96 SSV.dial CBV @11:22:42.98 CBV.dial SSV @11:22:43.83 Allowing 130s for 25.0ml of remaining 1450.0ml @11:22:43.84 Intake.select :open @11:22:46.70 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:22:46.72 SS.reconfigure SSinhale.with maxSpeed: 35 @11:22:46.76 SS.seek 25ml @11:23:09.28 delay 5 seconds @11:23:14.31 ATV.dial ASV @11:23:15.28 ASV.dial :SPEdelivery @11:23:15.30 AS.seek 0.02ml @11:23:37.33 Stroke completed -- waiting for pressure to equalize ... @11:23:43.08 Intake.select :close @11:23:43.24 SSV.dial :exhaust @11:23:44.08 Exhaust.select :open @11:23:44.20 SS.reconfigure SSexhale @11:23:44.25 SS.seek 0ml @11:23:53.53 Exhaust.select :close @11:23:53.65 SSV.dial CBV @11:23:53.67 CBV.dial SSV @11:23:54.50 Allowing 130s for 25.0ml of remaining 1425.0ml @11:23:54.51 Intake.select :open @11:23:56.70 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:23:56.72 SS.reconfigure SSinhale.with maxSpeed: 35 @11:23:56.76 SS.seek 25ml @11:24:47.20 Stroke completed -- waiting for pressure to equalize ... @11:24:53.60 Intake.select :close @11:24:53.76 SSV.dial :exhaust @11:24:54.60 Exhaust.select :open @11:24:54.72 SS.reconfigure SSexhale @11:24:54.78 SS.seek 0ml @11:25:03.69 Exhaust.select :close @11:25:03.81 SSV.dial CBV @11:25:03.83 CBV.dial SSV @11:25:04.69 Allowing 130s for 25.0ml of remaining 1400.0ml @11:25:04.70 Intake.select :open @11:25:06.89 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:25:06.90 SS.reconfigure SSinhale.with maxSpeed: 35 @11:25:06.95 SS.seek 25ml @11:25:40.34 Vacuum too high -- waiting up to 40s @11:25:42.21 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:25:42.23 SS.reconfigure SSinhale.with maxSpeed: 32 @11:25:42.28 SS.seek 25ml @11:26:01.30 Stroke completed -- waiting for pressure to equalize ... @11:26:07.11 Intake.select :close @11:26:07.28 SSV.dial :exhaust @11:26:08.13 Exhaust.select :open @11:26:08.25 SS.reconfigure SSexhale @11:26:08.30 SS.seek 0ml @11:26:17.39 Exhaust.select :close @11:26:17.51 SSV.dial CBV @11:26:17.53 CBV.dial SSV @11:26:18.39 Allowing 130s for 25.0ml of remaining 1375.0ml @11:26:18.40 Intake.select :open @11:26:20.59 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:26:20.61 SS.reconfigure SSinhale.with maxSpeed: 32 @11:26:20.65 SS.seek 25ml @11:26:32.68 delay 5 seconds @11:26:37.71 ATV.dial :air2 @11:26:38.75 AS.seek 0.52ml @11:27:14.38 Vacuum too high -- waiting up to 40s @11:27:16.52 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:27:16.54 SS.reconfigure SSinhale.with maxSpeed: 29 @11:27:16.59 SS.seek 25ml @11:27:18.07 Stroke completed -- waiting for pressure to equalize ... @11:27:22.56 Intake.select :close @11:27:22.72 SSV.dial :exhaust @11:27:23.56 Exhaust.select :open @11:27:23.68 SS.reconfigure SSexhale @11:27:23.73 SS.seek 0ml @11:27:32.71 Exhaust.select :close @11:27:32.83 SSV.dial CBV @11:27:32.85 CBV.dial SSV @11:27:33.71 Allowing 130s for 25.0ml of remaining 1350.0ml @11:27:33.72 Intake.select :open @11:27:35.91 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:27:35.93 SS.reconfigure SSinhale.with maxSpeed: 29 @11:27:35.97 SS.seek 25ml @11:28:19.61 delay 5 seconds @11:28:24.64 ATV.dial ASV @11:28:25.61 ASV.dial :wasteR @11:28:25.99 AS.seek 0.3ml @11:28:36.76 Stroke completed -- waiting for pressure to equalize ... @11:28:44.36 Intake.select :close @11:28:44.53 SSV.dial :exhaust @11:28:45.38 Exhaust.select :open @11:28:45.50 SS.reconfigure SSexhale @11:28:45.55 SS.seek 0ml @11:28:54.80 Exhaust.select :close @11:28:54.92 SSV.dial CBV @11:28:54.94 CBV.dial SSV @11:28:55.80 Allowing 130s for 25.0ml of remaining 1325.0ml @11:28:55.81 Intake.select :open @11:28:58.00 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:28:58.02 SS.reconfigure SSinhale.with maxSpeed: 29 @11:28:58.06 SS.seek 25ml @11:29:10.51 delay 20 seconds @11:29:30.55 AS.seek 0.07ml @11:29:50.66 Vacuum too high -- waiting up to 40s @11:29:53.04 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:29:53.06 SS.reconfigure SSinhale.with maxSpeed: 26 @11:29:53.11 SS.seek 25ml @11:30:02.27 Stroke completed -- waiting for pressure to equalize ... @11:30:08.68 Intake.select :close @11:30:08.84 SSV.dial :exhaust @11:30:09.68 Exhaust.select :open @11:30:09.80 SS.reconfigure SSexhale @11:30:09.85 SS.seek 0ml @11:30:17.09 delay 20 seconds @11:30:19.16 Exhaust.select :close @11:30:19.28 SSV.dial CBV @11:30:19.30 CBV.dial SSV @11:30:20.14 Allowing 130s for 25.0ml of remaining 1300.0ml @11:30:20.15 Intake.select :open @11:30:22.34 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:30:22.36 SS.reconfigure SSinhale.with maxSpeed: 26 @11:30:22.41 SS.seek 25ml @11:30:37.11 ATV.dial :waste2 @11:30:37.86 AS.reconfigure ASconfig @11:30:37.91 AS.seek :empty @11:30:42.31 Elution @11:30:42.32 ATV.dial :air1 @11:30:43.43 AS.reconfigure ASslow1 @11:30:43.48 AS.seek 0.049ml @11:30:53.90 delay 3 seconds @11:30:56.93 ATV.dial :ElutionWater @11:30:57.62 AS.seek 0.109ml @11:31:09.94 delay 3 seconds @11:31:12.97 ATV.dial ASV,via: :air2 @11:31:15.01 ASV.dial :SPEelution @11:31:15.59 AFV.select :carrierSolution @11:31:15.75 AS.reconfigure ASnorm @11:31:15.80 AS.seek 0.759ml @11:31:29.90 Stroke completed -- waiting for pressure to equalize ... @11:31:38.29 Intake.select :close @11:31:38.45 SSV.dial :exhaust @11:31:39.27 Exhaust.select :open @11:31:39.39 SS.reconfigure SSexhale @11:31:39.44 SS.seek 0ml @11:31:48.84 delay 3 seconds @11:31:49.11 Exhaust.select :close @11:31:49.23 SSV.dial CBV @11:31:49.25 CBV.dial SSV @11:31:50.11 Allowing 130s for 25.0ml of remaining 1275.0ml @11:31:50.13 Intake.select :open @11:31:51.86 AFV.select :holdingCoil @11:31:52.01 AS.reconfigure ASslow1 @11:31:52.07 AS.seek 0.474ml @11:31:52.31 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:31:52.33 SS.reconfigure SSinhale.with maxSpeed: 26 @11:31:52.37 SS.seek 25ml @11:32:33.82 Vacuum too high -- waiting up to 40s @11:32:36.47 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:32:36.49 SS.reconfigure SSinhale.with maxSpeed: 23 @11:32:36.54 SS.seek 25ml @11:32:49.64 delay 5 seconds @11:32:54.74 AS.reconfigure ASslow2 @11:32:54.79 AS.seek 0.439ml @11:33:06.12 Stroke completed -- waiting for pressure to equalize ... @11:33:15.03 Intake.select :close @11:33:15.19 SSV.dial :exhaust @11:33:16.04 Exhaust.select :open @11:33:16.16 SS.reconfigure SSexhale @11:33:16.21 SS.seek 0ml @11:33:25.60 Exhaust.select :close @11:33:25.72 SSV.dial CBV @11:33:25.74 CBV.dial SSV @11:33:27.29 Allowing 130s for 25.0ml of remaining 1250.0ml @11:33:27.30 Intake.select :open @11:33:29.48 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:33:29.50 SS.reconfigure SSinhale.with maxSpeed: 23 @11:33:29.55 SS.seek 25ml @11:33:30.00 delay 1:00 @11:34:25.07 Vacuum too high -- waiting up to 40s @11:34:28.27 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:34:28.29 SS.reconfigure SSinhale.with maxSpeed: 21 @11:34:28.33 SS.seek 25ml @11:34:30.03 pushing eluate to holding coil @11:34:30.11 AS.seek 0.279ml @11:34:51.37 Stroke completed -- waiting for pressure to equalize ... @11:35:01.02 Intake.select :close @11:35:01.18 SSV.dial :exhaust @11:35:02.03 Exhaust.select :open @11:35:02.16 SS.reconfigure SSexhale @11:35:02.21 SS.seek 0ml @11:35:11.35 Exhaust.select :close @11:35:11.47 SSV.dial CBV @11:35:11.49 CBV.dial SSV @11:35:12.33 Allowing 130s for 25.0ml of remaining 1225.0ml @11:35:12.34 Intake.select :open @11:35:14.53 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:35:14.55 SS.reconfigure SSinhale.with maxSpeed: 21 @11:35:14.59 SS.seek 25ml @11:36:05.60 Vacuum too high -- waiting up to 40s @11:36:09.71 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:36:09.73 SS.reconfigure SSinhale.with maxSpeed: 19 @11:36:09.78 SS.seek 25ml @11:36:46.36 Stroke completed -- waiting for pressure to equalize ... @11:36:59.12 Intake.select :close @11:36:59.28 SSV.dial :exhaust @11:37:00.10 Exhaust.select :open @11:37:00.22 SS.reconfigure SSexhale @11:37:00.27 SS.seek 0ml @11:37:09.29 Exhaust.select :close @11:37:09.42 SSV.dial CBV @11:37:09.44 CBV.dial SSV @11:37:10.29 Allowing 130s for 25.0ml of remaining 1200.0ml @11:37:10.30 Intake.select :open @11:37:11.17 delay 30 seconds @11:37:12.49 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:37:12.51 SS.reconfigure SSinhale.with maxSpeed: 19 @11:37:12.55 SS.seek 25ml @11:37:41.20 ATV.dial :waste,via: :air3 @11:37:42.86 AS.reconfigure ASconfig @11:37:42.91 AS.seek :empty @11:37:59.28 Parking Bleach in LysateSource line and SPEcolumn @11:37:59.29 ATV.dial :air3 @11:38:00.12 AS.reconfigure ASslow1 @11:38:00.17 AS.seek 0.029ml @11:38:00.57 Vacuum too high -- waiting up to 40s @11:38:04.26 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:38:04.29 SS.reconfigure SSinhale.with maxSpeed: 17 @11:38:04.34 SS.seek 25ml @11:38:06.41 ATV.dial :ElutionWater @11:38:07.03 AS.seek 0.059ml @11:38:14.07 ATV.dial :air3 @11:38:14.68 AS.seek 0.069ml @11:38:16.99 delay 2 seconds @11:38:19.01 ATV.dial :bleach @11:38:19.49 AS.seek 0.769ml @11:38:54.65 Stroke completed -- waiting for pressure to equalize ... @11:39:11.25 Intake.select :close @11:39:11.41 SSV.dial :exhaust @11:39:12.26 Exhaust.select :open @11:39:12.38 SS.reconfigure SSexhale @11:39:12.43 SS.seek 0ml @11:39:21.88 Exhaust.select :close @11:39:21.99 SSV.dial CBV @11:39:22.02 CBV.dial SSV @11:39:22.86 Allowing 130s for 25.0ml of remaining 1175.0ml @11:39:22.87 Intake.select :open @11:39:25.05 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:39:25.07 SS.reconfigure SSinhale.with maxSpeed: 17 @11:39:25.12 SS.seek 25ml @11:40:04.35 Vacuum too high -- waiting up to 40s @11:40:07.99 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:40:08.01 SS.reconfigure SSinhale.with maxSpeed: 15 @11:40:08.76 SS.seek 25ml @11:40:40.54 delay 3 seconds @11:40:43.57 ATV.dial :air3 @11:40:44.13 delay 3 seconds @11:40:47.22 AS.seek 0.794ml @11:40:52.49 delay 3 seconds @11:40:55.52 ATV.dial :bleach @11:40:56.12 AS.seek 0.779ml @11:40:59.43 delay 3 seconds @11:41:02.46 ATV.dial :AIV @11:41:03.69 AS.reconfigure ASconfig @11:41:03.74 AS.seek 0.015ml @11:41:10.92 Vacuum too high -- waiting up to 40s @11:41:15.99 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:41:16.01 SS.reconfigure SSinhale.with maxSpeed: 14 @11:41:16.06 SS.seek 25ml @11:41:28.28 Stroke completed -- waiting for pressure to equalize ... @11:41:32.88 SS.reconfigure SSconfig @11:41:32.93 SS.hold @11:41:33.06 Intake.select :close @11:41:33.22 ShallowSampler::Clogged in WCR -- progress too slow after 350.0ml @11:41:33.24 SSV.dial :exhaust @11:41:34.07 Exhaust.select :open @11:41:34.19 SS.reconfigure SSexhale @11:41:34.24 SS.seek 0ml @11:41:43.69 Exhaust.select :close @11:41:43.80 Sampled 350.0ml @11:41:43.82 Drying puck before fixing for 4:00 @11:41:43.83 CBV.dial :waste2b @11:41:44.65 delay 1:00 @11:41:47.97 ATV.dial :waste @11:41:49.41 AS.seek :empty @11:41:50.63 ATV.dial :air3 @11:41:51.29 AS.reconfigure ASslow1 @11:41:51.34 AS.seek 0.009ml @11:41:53.63 delay 2 seconds @11:41:55.65 ATV.dial :bleach @11:41:56.11 AS.seek 0.579ml @11:42:44.68 CSV.dial :air @11:42:45.15 CS.reconfigure CSfast @11:42:45.20 CS.seek 10ml @11:43:10.01 Pumping wcr puck from tube 3 up to 55psi... @11:43:10.02 CBV.dial SSV @11:43:12.11 CBV.dial between CSV and waste2b @11:43:13.18 CSV.dial between air and CTV @11:43:13.54 CS.seek 7.184ml @11:43:21.21 CTV.dial CSV,avoiding: :air @11:43:21.24 CSV.dial CTV @11:43:22.12 CS.reconfigure CSpressurize @11:43:22.17 CS.seek 0ml @11:43:41.14 CSV.dial :air @11:43:41.65 CS.reconfigure CSfast @11:43:41.70 CS.seek 10ml @11:43:51.01 delay 3 seconds @11:43:54.04 ATV.dial :air3 @11:43:54.58 delay 3 seconds @11:43:57.67 AS.seek 0.604ml @11:44:02.97 delay 3 seconds @11:44:06.00 ATV.dial :bleach @11:44:06.40 CSV.dial between air and CTV @11:44:06.61 AS.seek 0.589ml @11:44:06.76 CS.seek 2.547ml @11:44:09.90 delay 3 seconds @11:44:12.93 ATV.dial :ASV @11:44:13.93 ASV.dial :wasteR @11:44:14.52 AS.seek 0ml @11:44:25.46 CTV.dial CSV,avoiding: :air @11:44:25.48 CSV.dial CTV @11:44:25.82 CS.reconfigure CSpressurize @11:44:25.87 CS.seek 0ml @11:44:30.48 CBV.dial :waste2b @11:44:30.83 CS.reconfigure CSconfig @11:44:30.88 CS.seek :empty @11:44:34.23 delay 1:00 @11:45:34.26 CTV.dial :air,avoiding: :intake @11:45:35.69 Fix #1 of 2 (with rnal) for 2:00 @11:45:35.71 CSV.dial CSR @11:45:36.25 Pulling 2ml of CSR.rnal @11:45:36.27 CSR.select :rnal @11:45:36.39 CS.seek 2ml @11:45:42.61 delay 5 seconds @11:45:47.64 CSR.select :air @11:45:47.77 CS.reconfigure CSfast @11:45:47.82 CS.seek 10ml @11:46:07.67 Pumping wcr puck from tube 3 up to 55psi... @11:46:07.69 CBV.dial SSV @11:46:09.09 CBV.dial between CSV and waste2b @11:46:10.17 CSV.dial between air and CTV @11:46:10.84 CS.seek 7.189ml @11:46:13.27 delay 1:00 @11:46:18.56 CTV.dial CSV,avoiding: :air @11:46:18.58 CSV.dial CTV @11:46:20.12 CS.reconfigure CSpressurize @11:46:20.18 CS.seek 0ml @11:46:39.14 CSV.dial :air @11:46:39.66 CS.reconfigure CSfast @11:46:40.39 CS.seek 10ml @11:47:04.93 CSV.dial between air and CTV @11:47:05.29 CS.seek 2.253ml @11:47:13.30 AFV.select :carrierSolution @11:47:13.40 delay 2 seconds @11:47:15.43 AS.reconfigure ASconfig @11:47:15.48 AS.seek 1ml @11:47:24.87 CTV.dial CSV,avoiding: :air @11:47:24.89 CSV.dial CTV @11:47:25.23 CS.reconfigure CSpressurize @11:47:25.28 CS.seek 0ml @11:47:27.85 delay 2:00 @11:48:13.24 delay 1 second @11:48:14.26 AFV.select :holdingCoil @11:48:14.36 delay 1 second @11:48:15.39 ATV.dial ASV @11:48:15.40 ASV.dial :wasteR @11:48:15.42 AS.reconfigure ASslow1 @11:48:15.47 AS.seek 0ml @11:49:27.89 CBV.dial :waste2b @11:49:28.25 CS.reconfigure CSconfig @11:49:28.30 CS.seek :empty @11:49:33.03 delay 1:00 @11:50:33.06 CTV.dial :air,avoiding: :intake @11:50:34.49 Fix #2 of 2 (with rnal) for 2:00 @11:50:34.51 CSV.dial CSR @11:50:35.06 Pulling 2ml of CSR.rnal @11:50:35.07 CSR.select :rnal @11:50:35.19 CS.seek 2ml @11:50:41.27 delay 5 seconds @11:50:46.30 CSR.select :air @11:50:46.43 CS.reconfigure CSfast @11:50:46.48 CS.seek 10ml @11:51:06.08 Pumping wcr puck from tube 3 up to 55psi... @11:51:06.10 CBV.dial SSV @11:51:07.47 CBV.dial between CSV and waste2b @11:51:08.53 CSV.dial between air and CTV @11:51:09.20 CS.seek 7.176ml @11:51:16.94 CTV.dial CSV,avoiding: :air @11:51:17.01 CSV.dial CTV @11:51:18.44 CS.reconfigure CSpressurize @11:51:18.49 CS.seek 0ml @11:51:36.85 delay 20 seconds @11:51:37.49 CSV.dial :air @11:51:37.99 CS.reconfigure CSfast @11:51:38.05 CS.seek 10ml @11:51:56.88 ATV.dial :air3 @11:51:58.33 AS.seek 0.59ml @11:52:02.77 CSV.dial between air and CTV @11:52:03.13 CS.seek 2.273ml @11:52:22.44 CTV.dial CSV,avoiding: :air @11:52:22.46 CSV.dial CTV @11:52:22.81 CS.reconfigure CSpressurize @11:52:22.86 CS.seek 0ml @11:52:25.51 delay 2:00 @11:53:57.28 delay 3 seconds @11:54:00.31 ATV.dial :ASV @11:54:01.65 ASV.dial :wasteR @11:54:01.73 AS.seek 0.09ml @11:54:25.54 CBV.dial :waste2b @11:54:25.91 CS.reconfigure CSconfig @11:54:26.64 CS.seek :empty @11:54:31.27 delay 1:00 @11:55:31.31 CTV.dial :air,avoiding: :intake @11:55:32.81 Storing wcr puck in Collection Clamp to tube 2 @11:55:33.01 SC.reconfigure SCtight @11:55:33.06 SC.seek 2 @11:55:35.28 SC.reconfigure SCloose @11:55:35.52 Elbow.reconfigure TightElbow @11:55:35.58 Elbow.seek 2 @11:55:37.53 Forearm.reconfigure TightForearm @11:55:37.58 Forearm.seek 2 + 700 counts @11:55:38.61 SE.reconfigure SEpick @11:55:38.69 SE.seek beamBreak @11:55:40.94 SE.configure SEconfig @11:55:41.12 Elbow.reconfigure LimpElbow @11:55:41.18 Forearm.reconfigure LooseForearm @11:55:41.22 Forearm.seek 2 @11:55:41.53 Elbow.hold @11:55:41.61 Elbow.reconfigure TightElbow @11:55:41.72 SE.seek 10.07 Pucks below Top Plate @11:55:42.30 Forearm.force -20 @11:55:42.35 Forearm.seek 2 + 341 counts @11:55:42.51 delay 30 seconds @11:55:42.70 Forearm.reconfigure TightForearm @11:55:42.75 Forearm.seek 2 + 700 counts @11:55:43.04 SE.seek 11.07 Pucks below Top Plate @11:55:43.66 Forearm.reconfigure LooseForearm @11:55:43.71 Forearm.seek :retract @11:55:44.62 Elbow.seek CC @11:55:46.36 Elbow.reconfigure LooseElbow @11:55:46.46 Forearm.seek CC @11:55:47.51 Hand.close @11:55:48.23 CC.seek :open @11:55:52.72 Forearm.seek :retract @11:55:53.73 Elbow.reconfigure TightElbow @11:55:53.78 Elbow.seek 2 @11:55:55.78 Elbow.reconfigure LimpElbow @11:55:55.84 Forearm.seek 2 @11:55:56.81 Elbow.hold @11:55:56.89 Elbow.reconfigure TightElbow @11:55:56.94 Hand.open @11:55:57.75 Forearm.force -20 @11:55:58.45 Forearm.seek 2 + 342 counts @11:55:58.82 Forearm.reconfigure TightForearm @11:55:58.87 Forearm.seek 2 + 700 counts @11:55:59.29 SE.seek :down @11:56:00.43 Forearm.reconfigure LooseForearm @11:56:00.48 Forearm.seek :retract @11:56:00.97 SE.coast @11:56:01.39 SC.coast @11:56:01.44 Starting finalPurge @11:56:01.45 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:56:01.53 Hand.open @11:56:01.87 Elbow.seek FlushPuck::Garage @11:56:03.97 Elbow.reconfigure LooseElbow @11:56:04.08 Forearm.seek :garage @11:56:05.09 Hand.close @11:56:05.87 Forearm.seek :retract @11:56:06.87 Elbow.reconfigure TightElbow @11:56:06.92 Elbow.seek CC @11:56:09.88 Elbow.reconfigure LooseElbow @11:56:09.98 Forearm.reconfigure TightForearm @11:56:10.03 Forearm.seek CC @11:56:11.37 CC.seek :closed @11:56:12.57 ATV.dial :extSrc @11:56:13.34 delay 3 seconds @11:56:15.62
Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.05 @11:56:15.70 Hand.open @11:56:16.43 Forearm.reconfigure LooseForearm @11:56:16.48 Forearm.seek :retract @11:56:16.50 ATV.dial :ElutionWater @11:56:17.51
Flushing the Collection Valve::Manifold @11:56:17.73 CSV.dial CSR @11:56:17.78 AS.seek 0.12ml @11:56:18.35
Pulling 5ml of CSR.flush CSR.select :flush @11:56:18.48 CS.seek 5ml @11:56:24.17 ATV.dial :air3 @11:56:24.77 AS.seek 0.13ml @11:56:27.11 delay 2 seconds @11:56:29.13 ATV.dial :bleach @11:56:29.60 AS.seek 0.23ml @11:56:32.25
delay 5 seconds @11:56:37.28 CSR.select :air @11:56:37.44 CS.reconfigure CSfast @11:56:37.49 CS.seek 7.5ml @11:56:44.31 CSV.dial :waste @11:56:45.70 CS.reconfigure CSconfig @11:56:45.75 CS.seek 0ml @11:56:50.03 delay 3 seconds @11:56:53.06 ATV.dial :air3 @11:56:53.59 delay 3 seconds @11:56:56.68 AS.seek 0.255ml @11:57:01.98 delay 3 seconds @11:57:05.01 ATV.dial :bleach @11:57:05.61 AS.seek 0.24ml @11:57:06.59
Flushing lower manifold's waste1 port @11:57:06.73 CSV.dial :flush @11:57:07.24 CS.reconfigure CSfast @11:57:07.28 CS.seek 5ml @11:57:08.90 delay 3 seconds @11:57:11.92 ATV.dial :ASV @11:57:12.92 ASV.dial :SPEdelivery @11:57:13.29 AFV.select :carrierSolution @11:57:13.40 delay 2 seconds @11:57:15.43 AS.reconfigure ASconfig @11:57:15.48 AS.seek 0.5ml @11:57:20.12
delay 5 seconds @11:57:25.15 CSV.dial :air @11:57:25.84 CS.seek 7ml @11:57:30.72 delay 1 second @11:57:31.59 CSV.dial CTV @11:57:31.63
CTV.dial CSV @11:57:31.74 AFV.select :holdingCoil @11:57:31.89 AS.reconfigure ASslow1 @11:57:31.95 AS.seek 0.17ml @11:57:33.12
CBV.dial :waste1 @11:57:33.94 CS.seek 0ml @11:57:51.31 Flushing lower manifold's waste2b port @11:57:51.45 CSV.dial :flush @11:57:52.27 CS.seek 5ml @11:58:05.03 delay 5 seconds @11:58:10.06 CSV.dial :air @11:58:10.63 CS.seek 7ml @11:58:16.34 CSV.dial CTV @11:58:16.37
CTV.dial CSV @11:58:16.86 CBV.dial :waste2b @11:58:17.70 CS.seek 0ml @11:58:35.03 Flushing upper manifold @11:58:35.17 CSV.dial :flush @11:58:35.98 CS.seek 5ml @11:58:38.59 SPE.hold 2:46:40,95C @11:58:38.64 delay 3:00 @11:58:49.12
delay 5 seconds @11:58:54.15 CSV.dial :air @11:58:54.74 CS.seek 7ml @11:59:00.56 CSV.dial CBV @11:59:00.60
CBV.dial CSV @11:59:01.81 CTV.dial :air @11:59:03.29 CS.seek 0ml @11:59:21.37 delay 7 seconds @11:59:28.41 CS.seek 3ml @11:59:36.97 CSV.dial :waste @11:59:37.49 CS.seek 0ml @11:59:45.96 CSV.dial :flush @11:59:46.46 CS.seek 3ml @11:59:54.78 delay 5 seconds @11:59:59.81 CSV.dial :air @12:00:00.37 CS.seek 5ml @12:00:06.25 CSV.dial :air @12:00:06.27 CS.seek 0ml @12:00:19.66 delay 7 seconds @12:00:26.70 CS.seek 3ml @12:00:35.03 CSV.dial :waste @12:00:35.85 CS.seek 0ml @12:00:44.25 Disinfecting external Intake with mfbkill @12:00:44.39 CSV.dial CSR @12:00:45.89 Pulling 8ml of CSR.mfbkill @12:00:45.90 CSR.select :mfbkill @12:00:46.71 CS.seek 8ml @12:01:07.41 delay 5 seconds @12:01:12.44 CSR.select :air @12:01:12.57 CS.seek 10ml @12:01:18.47 CSV.dial CBV @12:01:18.51
CBV.dial CSV @12:01:19.66 CTV.dial :intake @12:01:20.52 Intake.select :open @12:01:20.64 CS.seek 2ml @12:01:38.70 AFV.select :carrierSolution @12:01:38.80 delay 2 seconds @12:01:40.83 AS.reconfigure ASconfig @12:01:40.88 AS.seek 1ml @12:01:42.28
Intake.select :close @12:01:42.39 CTV.dial :air @12:01:43.24 CS.reconfigure CSconfig @12:01:43.29 CS.seek 0ml @12:01:49.44 Flushing lower manifold's waste1 port @12:01:49.45 CSV.dial CSR @12:01:50.68 Pulling 4ml of CSR.mfbkill @12:01:50.69 CSR.select :mfbkill @12:01:50.81 CS.seek 4ml @12:02:02.00 delay 5 seconds @12:02:07.03 CSR.select :air @12:02:07.16 CS.reconfigure CSfast @12:02:07.20 CS.seek 10ml @12:02:22.88 CSV.dial CTV @12:02:22.91
CTV.dial CSV @12:02:24.49 CBV.dial :waste1 @12:02:25.01 CS.seek 0ml @12:02:28.86 delay 1 second @12:02:29.88 AFV.select :holdingCoil @12:02:29.98 delay 1 second @12:02:31.01 ASV.dial :wasteD @12:02:32.12 AS.seek 0ml @12:02:50.38
Flushing lower manifold's waste2b port @12:02:50.39 CSV.dial CSR @12:02:50.96 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @12:02:51.09 CS.reconfigure CSconfig @12:02:51.14 CS.seek 4ml @12:03:02.32 delay 5 seconds @12:03:07.35 CSR.select :air @12:03:07.48 CS.reconfigure CSfast @12:03:07.52 CS.seek 10ml @12:03:23.10 CSV.dial CTV @12:03:23.13
CTV.dial CSV @12:03:23.64 CBV.dial :waste2b @12:03:24.51 CS.seek 0ml @12:03:29.91 Priming Eluate @12:03:29.92 AFV.select :carrierSolution @12:03:30.03 delay 2 seconds @12:03:32.07 AS.seek 0.5ml @12:03:49.63
Killing upper manifold @12:03:49.64 CSV.dial CSR @12:03:50.19 Pulling 3.999ml of CSR.mfbkill @12:03:50.20 CSR.select :mfbkill @12:03:50.33 CS.reconfigure CSconfig @12:03:50.38 CS.seek 4ml @12:04:01.09 delay 1 second @12:04:01.60
delay 5 seconds @12:04:02.11 AFV.select :holdingCoil @12:04:02.21 ATV.dial ASV @12:04:02.22 ASV.dial :SPErecovery @12:04:02.81 Swish #1 of 2 @12:04:02.89 AS.reconfigure ASslow0 @12:04:02.94 AS.seek 0.6ml @12:04:06.62
CSR.select :air @12:04:06.74 CS.reconfigure CSfast @12:04:06.78 CS.seek 10ml @12:04:13.38 AS.seek 0.5ml @12:04:22.19 CSV.dial CBV @12:04:22.22
CBV.dial CSV @12:04:23.39 CTV.dial :air @12:04:23.77 Swish #2 of 2 @12:04:23.86 AS.seek 0.6ml @12:04:24.83
CS.seek 0ml @12:04:34.32 AS.seek 0.5ml @12:04:44.69 delay 3 seconds @12:04:47.77 AS.reconfigure ASslow1 @12:04:47.83 AS.seek 0.684ml @12:04:49.57
delay 7 seconds @12:04:56.61 CS.seek 3ml @12:05:04.72 CSV.dial :waste @12:05:05.20 CS.seek 0ml @12:05:14.04 Flushing upper manifold @12:05:14.18 CSV.dial :flush @12:05:14.67 CS.seek 5ml @12:05:25.14 delay 3 seconds @12:05:27.63
delay 5 seconds @12:05:28.16 Eluate primed @12:05:28.17 ATV.dial :waste2 @12:05:28.78 AS.reconfigure ASnorm @12:05:28.83 AS.seek 0ml @12:05:32.65
CSV.dial :air @12:05:33.21 CS.seek 7ml @12:05:39.01 CSV.dial CBV @12:05:39.05
CBV.dial CSV @12:05:40.30 CTV.dial :air @12:05:40.32 CS.seek 0ml @12:05:57.94 delay 7 seconds @12:06:03.64 SPE.coast @12:06:03.72 ASV.dial :SPErecovery @12:06:03.74 Setting up PCR for OM -- writing /var/log/esp/OM20may2312h325ml.pcr @12:06:03.78 Building PCR Slug from ASV with MM3 mix, PP5 primer @12:06:03.79 Priming with carrier @12:06:03.80 ATV.dial ARV @12:06:04.75 ARV.dial :carrier @12:06:04.97
CS.seek 3ml @12:06:05.12 AS.seek 0.5ml @12:06:12.94
CSV.dial :waste @12:06:13.42 CS.seek 0ml @12:06:21.61 CSV.dial :flush @12:06:22.08 CS.seek 3ml @12:06:30.04 delay 5 seconds @12:06:30.72 AFV.select :carrierSolution @12:06:30.83 delay 2 seconds @12:06:32.86 AS.reconfigure ASconfig @12:06:32.91 AS.seek 1ml @12:06:35.06
CSV.dial :air @12:06:35.62 CS.seek 5ml @12:06:41.26 CSV.dial :air @12:06:41.28 CS.seek 0ml @12:06:53.85 delay 7 seconds @12:07:00.89 CS.seek 3ml @12:07:01.92 delay 1 second @12:07:02.94 AFV.select :holdingCoil @12:07:03.04 delay 1 second @12:07:04.06 ATV.dial :waste @12:07:05.47 AS.reconfigure ASnorm @12:07:05.52 AS.seek :empty @12:07:08.88
CSV.dial :waste @12:07:09.67 CS.seek 0ml @12:07:17.70 Flushing lower manifold's waste1 port @12:07:17.84 CSV.dial :flush @12:07:18.35 CS.seek 5ml @12:07:30.91 delay 5 seconds @12:07:35.94 CSV.dial :air @12:07:36.48 CS.seek 7ml @12:07:42.29 CSV.dial CTV @12:07:42.33
CTV.dial CSV @12:07:43.82 CBV.dial :waste1 @12:07:44.31 CS.seek 0ml @12:07:56.25 AFV.select :carrierSolution @12:07:56.36 delay 2 seconds @12:07:58.39 AS.reconfigure ASconfig @12:07:58.44 AS.seek 0.5ml @12:08:01.57
Flushing lower manifold's waste2b port @12:08:01.71 CSV.dial :flush @12:08:02.52 CS.seek 5ml @12:08:15.17 delay 5 seconds @12:08:20.20 CSV.dial :air @12:08:20.88 CS.seek 7ml @12:08:26.67 CSV.dial CTV @12:08:26.70
CTV.dial CSV @12:08:27.20 CBV.dial :waste2b @12:08:27.52 delay 1 second @12:08:28.03
CS.seek 0ml @12:08:28.54 AFV.select :holdingCoil @12:08:28.65 Building Slug @12:08:28.66 ATV.dial :ARV @12:08:30.89 delay 2 seconds @12:08:32.92 ARV.dial :air1 @12:08:33.30 delay 2 seconds @12:08:35.39 AS.reconfigure ASslow2 @12:08:35.44 AS.seek 0.506ml @12:08:41.89 delay 2 seconds @12:08:43.92 ARV.dial :MM3 @12:08:44.93 delay 2 seconds @12:08:45.32
Disinfecting external Intake with kill @12:08:45.46 CSV.dial CSR @12:08:46.02 Pulling 8ml of CSR.kill @12:08:46.03 CSR.select :kill @12:08:46.15 CS.seek 8ml @12:08:46.96 Adding MM3 @12:08:47.05 AS.seek 0.524ml @12:09:05.49 delay 25 seconds @12:09:05.67
delay 5 seconds @12:09:10.70 CSR.select :air @12:09:10.83 CS.seek 10ml @12:09:16.48 CSV.dial CBV @12:09:16.51
CBV.dial CSV @12:09:17.65 CTV.dial :intake @12:09:18.45 Intake.select :open @12:09:18.57 CS.seek 2ml @12:09:30.52 ARV.dial :PP5 @12:09:30.91 Adding PP5 @12:09:30.93 delay 2 seconds @12:09:33.02 AS.seek 0.53ml @12:09:39.20
Intake.select :close @12:09:39.31 CTV.dial :air @12:09:39.47 delay 10 seconds @12:09:40.12
CS.reconfigure CSconfig @12:09:40.17 CS.seek 0ml @12:09:46.29 CSV.dial CSR @12:09:47.44 Pulling 5ml of CSR.flush CSR.select :flush @12:09:47.57 CS.seek 5ml @12:09:49.50 ARV.dial :air3 @12:09:49.88 adding ARV air @12:09:49.90 delay 2 seconds @12:09:51.98 AS.seek 0.536ml @12:09:58.39 delay 5 seconds @12:10:01.42
delay 5 seconds @12:10:03.41 ARV.dial :carrier @12:10:04.63 delay 7 seconds @12:10:06.45
CSR.select :air @12:10:06.57 CS.seek 10ml @12:10:11.72 AS.seek 0.594ml @12:10:20.26
CSV.dial CBV @12:10:21.35 CS.seek 0ml @12:10:47.82 Garaging FlushPuck in Collection Clamp @12:10:47.90 Elbow.reconfigure TightElbow @12:10:47.95 Elbow.seek CC @12:10:48.22 Elbow.reconfigure LooseElbow @12:10:48.32 Forearm.seek CC @12:10:49.35 Hand.close @12:10:50.01 CC.seek :open @12:10:54.42 Forearm.seek :retract @12:10:55.41 Elbow.reconfigure TightElbow @12:10:55.46 Elbow.seek :garage @12:10:58.47 Elbow.reconfigure LooseElbow @12:10:58.57 Forearm.reconfigure TightForearm @12:10:58.62 Forearm.seek :garage @12:10:59.76 Hand.open @12:11:00.58 Forearm.reconfigure LooseForearm @12:11:00.63 Forearm.seek :retract @12:11:01.68 Starting initialPurge @12:11:01.69 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:11:01.77 Hand.open @12:11:02.08 Elbow.reconfigure TightElbow @12:11:02.84 Elbow.seek FlushPuck::Garage @12:11:02.98 Elbow.reconfigure LooseElbow @12:11:03.08 Forearm.seek :garage @12:11:04.10 Hand.close @12:11:04.91 Forearm.seek :retract @12:11:05.91 Elbow.reconfigure TightElbow @12:11:05.96 Elbow.seek CC @12:11:08.89 Elbow.reconfigure LooseElbow @12:11:08.99 Forearm.reconfigure TightForearm @12:11:09.04 Forearm.seek CC @12:11:10.39 CC.seek :closed @12:11:10.48 delay 5 seconds @12:11:14.64
Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.07 @12:11:14.72 Hand.open @12:11:15.46 Forearm.reconfigure LooseForearm @12:11:15.51 Forearm.seek :retract @12:11:15.56 ATV.dial :ASV @12:11:15.94 delay 2 seconds @12:11:16.52
Flushing the Collection Valve::Manifold @12:11:16.66 CSV.dial CSR @12:11:17.81 Pulling 5ml of CSR.flush @12:11:17.82 CSR.select :flush @12:11:17.95 CS.seek 5ml @12:11:18.07 AS.seek 0.6ml @12:11:24.36 delay 4 seconds @12:11:28.39 ATV.dial ARV @12:11:28.91 delay 2 seconds @12:11:30.94 ARV.dial :carrier @12:11:30.95 delay 2 seconds @12:11:31.76
delay 5 seconds @12:11:33.05 AS.seek 0.65ml @12:11:36.78
CSR.select :air @12:11:36.94 CS.reconfigure CSfast @12:11:36.99 CS.seek 7.5ml @12:11:43.79 CSV.dial :waste @12:11:45.17 CS.reconfigure CSconfig @12:11:45.22 CS.seek 0ml @12:12:05.36 Purging external Intake @12:12:05.49 CSV.dial :flush @12:12:05.98 CS.reconfigure CSfast @12:12:06.03 CS.seek 10ml @12:12:23.71 delay 4 seconds @12:12:27.74 ATV.dial PCR @12:12:28.32 ASV.dial :waste1 @12:12:29.09 ARV.dial :air1 @12:12:29.47 Positioning Slug by 0.324ml @12:12:29.56 AS.seek 0.326ml @12:12:30.17
delay 5 seconds @12:12:35.19 CSV.dial CBV @12:12:35.22
CBV.dial CSV @12:12:36.01 CTV.dial :intake @12:12:36.81 Intake.select :open @12:12:36.93 CS.seek 0ml @12:13:02.48 Intake.select :close @12:13:02.59 CTV.dial :air @12:13:03.39 Flushing upper manifold @12:13:03.52 CSV.dial :flush @12:13:04.32 CS.seek 5ml @12:13:16.83 delay 5 seconds @12:13:21.86 CSV.dial :air @12:13:22.40 CS.seek 7ml @12:13:28.05 CSV.dial CBV @12:13:28.82
CBV.dial CSV @12:13:30.03 CTV.dial :air @12:13:30.05 CS.seek 0ml @12:13:47.36 delay 7 seconds @12:13:54.40 CS.seek 3ml @12:14:02.39 CSV.dial :waste @12:14:02.98 CS.seek 0ml @12:14:11.24 CSV.dial :flush @12:14:11.74 CS.seek 3ml @12:14:19.70 delay 5 seconds @12:14:24.73 CSV.dial :air @12:14:25.27 CS.seek 5ml @12:14:30.95 CSV.dial :air @12:14:30.97 CS.seek 0ml @12:14:43.70 delay 7 seconds @12:14:50.74 CS.seek 3ml @12:14:58.80 CSV.dial :waste @12:14:59.60 CS.seek 0ml @12:15:07.70 Garaging FlushPuck in Collection Clamp @12:15:07.78 Elbow.reconfigure TightElbow @12:15:07.83 Elbow.seek CC @12:15:08.07 Elbow.reconfigure LooseElbow @12:15:08.17 Forearm.seek CC @12:15:09.19 Hand.close @12:15:09.77 CC.seek :open @12:15:14.15 Forearm.seek :retract @12:15:15.14 Elbow.reconfigure TightElbow @12:15:15.19 Elbow.seek :garage @12:15:18.31 Elbow.reconfigure LooseElbow @12:15:18.42 Forearm.reconfigure TightForearm @12:15:18.46 Forearm.seek :garage @12:15:19.61 Hand.open @12:15:20.34 Forearm.reconfigure LooseForearm @12:15:20.39 Forearm.seek :retract @12:15:21.41 Completed [stdphase] begun 10:01:17 @12:15:21.42 Preparing to sleep until Sun May 24 10:01:17 +0200 2020 @12:15:21.48 deferred until MFBPCR finish @12:15:21.51
Waiting for MFBPCR til 10:01CEST24-May-20 @12:15:21.52 Continuing at 10:01CEST24-May-20 @12:15:21.59 GoodNews.email "Then, in tube 3: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:150\n{1 => 22, 2 => 12, 3 => 10}\nCan@12:15:21, 10.0C, 49% humidity, 14.4psia, 13.225V, 0.500A, 0.500A avg, 6.61W",Subject: "Waiting until 10:01CEST24-May-20" @12:17:55.57 delay 5 seconds @12:18:00.60 ATV.dial ARV @12:18:01.34 ASV.dial :PCRlock @12:18:01.71 delay 5 seconds @12:18:06.74 Running OM Profile60C350 thermocycling @12:18:06.75 PCR.program Profile60C350 @12:18:06.87 Profile60C350 should complete in < 2:02:45 @12:18:06.88 PCR.run 2:38:26.25 @13:54:42.72 PCR.off @13:54:43.99 PCR for OM completed -- closed /var/log/esp/OM20may2312h325ml.pcr @13:54:44.07 Cleaning PCR @13:54:44.08 AFV.select :carrierSolution @13:54:44.19 delay 2 seconds @13:54:46.22 AS.reconfigure ASconfig @13:54:46.27 AS.seek 0.5ml @13:54:56.57 delay 1 second @13:54:57.59 AFV.select :holdingCoil @13:54:57.69 ASV.dial :waste1 @13:54:58.07 ATV.dial :air3 @13:54:59.31 AS.reconfigure ASslow1 @13:54:59.36 AS.seek 0.51ml @13:55:01.67 delay 2 seconds @13:55:03.69 ATV.dial :bleach @13:55:04.29 AS.seek 0.81ml @13:56:04.88 delay 3 seconds @13:56:07.91 ATV.dial :air3 @13:56:08.43 delay 3 seconds @13:56:11.52 AS.seek 0.835ml @13:56:16.80 delay 3 seconds @13:56:19.83 ATV.dial :bleach @13:56:20.42 AS.seek 0.82ml @13:56:23.68 delay 3 seconds @13:56:26.71 ARV.dial :waste @13:56:27.29 delay 3 seconds @13:56:30.32 ATV.dial :ARV @13:56:31.28 AS.reconfigure ASconfig @13:56:31.33 AS.seek 0.47ml @13:56:51.73 AFV.select :carrierSolution @13:56:51.85 delay 2 seconds @13:56:53.89 AS.seek 1ml @13:57:24.63 delay 1 second @13:57:25.65 AFV.select :holdingCoil @13:57:25.75 delay 1 second @13:57:26.78 AS.reconfigure ASfast @13:57:26.83 AS.seek 0ml @13:57:54.16 AFV.select :carrierSolution @13:57:54.27 delay 2 seconds @13:57:56.30 AS.reconfigure ASconfig @13:57:56.35 AS.seek 1ml @13:58:54.12 delay 1 second @13:58:55.14 AFV.select :holdingCoil @13:58:55.24 delay 1 second @13:58:56.27 AS.reconfigure ASfast @13:58:56.32 AS.seek 0ml @13:59:23.65 AFV.select :carrierSolution @13:59:23.76 delay 2 seconds @13:59:25.79 AS.reconfigure ASconfig @13:59:25.92 AS.seek 1ml @14:00:23.76 delay 1 second @14:00:24.78 AFV.select :holdingCoil @14:00:24.88 delay 1 second @14:00:25.91 AS.reconfigure ASfast @14:00:25.96 AS.seek 0ml @14:00:53.32 ARV.dial between carrier and air1 @14:00:53.81 ATV.dial :air3 @14:00:55.03 AS.reconfigure ASslow1 @14:00:55.08 AS.seek 0.01ml @14:00:57.37 delay 2 seconds @14:00:59.39 ATV.dial :bleach @14:01:00.00 AS.seek 0.81ml @14:03:41.14 delay 3 seconds @14:03:44.17 ATV.dial :air3 @14:03:44.71 delay 3 seconds @14:03:47.80 AS.seek 0.835ml @14:03:53.08 delay 3 seconds @14:03:56.11 ATV.dial :bleach @14:03:56.72 AS.seek 0.82ml @14:04:00.00 delay 3 seconds @14:04:03.03 ATV.dial PCR @14:04:03.43 AS.reconfigure ASconfig @14:04:03.48 AS.seek :empty @14:04:50.95 PCR.program :bleachProfile @14:04:51.01 PCR program should complete in < 2:45 @14:04:51.02 PCR.run 10:00 @14:08:04.96 PCR.off @14:08:04.99 AFV.select :carrierSolution @14:08:05.10 delay 2 seconds @14:08:07.14 AS.seek 1ml @14:09:05.01 delay 1 second @14:09:06.03 AFV.select :holdingCoil @14:09:06.13 delay 1 second @14:09:07.16 AS.reconfigure ASfast @14:09:07.21 AS.seek 0ml @14:09:34.58 delay 3 seconds @14:09:37.61 AFV.select :carrierSolution @14:09:37.71 delay 2 seconds @14:09:39.74 AS.reconfigure ASconfig @14:09:39.83 AS.seek 1ml @14:10:37.61 delay 1 second @14:10:38.63 AFV.select :holdingCoil @14:10:38.73 delay 1 second @14:10:39.76 AS.reconfigure ASfast @14:10:39.81 AS.seek 0ml @14:11:07.16 delay 3 seconds @14:11:10.19 AFV.select :carrierSolution @14:11:10.29 delay 2 seconds @14:11:12.32 AS.reconfigure ASconfig @14:11:12.38 AS.seek 1ml @14:12:10.13 delay 1 second @14:12:11.15 AFV.select :holdingCoil @14:12:11.28 delay 1 second @14:12:12.31 AS.reconfigure ASfast @14:12:12.36 AS.seek 0ml @14:12:39.72 delay 3 seconds @14:12:42.75 AFV.select :carrierSolution @14:12:42.85 delay 2 seconds @14:12:44.88 AS.reconfigure ASconfig @14:12:44.93 AS.seek 1ml @14:13:42.72 delay 1 second @14:13:43.74 AFV.select :holdingCoil @14:13:43.84 delay 1 second @14:13:44.87 AS.reconfigure ASfast @14:13:44.92 AS.seek 0ml @14:14:12.28 delay 3 seconds @14:14:15.31 AFV.select :carrierSolution @14:14:15.41 delay 2 seconds @14:14:17.44 AS.reconfigure ASconfig @14:14:17.49 AS.seek 1ml @14:15:15.35 delay 1 second @14:15:16.37 AFV.select :holdingCoil @14:15:16.47 delay 1 second @14:15:17.50 AS.reconfigure ASnorm @14:15:17.55 AS.seek 0ml @14:16:08.27 Setting up PCR for ML -- writing /var/log/esp/ML20may2314h325ml.pcr @14:16:08.29 Building PCR Slug from ASV with MM2 mix, PP4 primer @14:16:08.30 Priming with carrier @14:16:08.31 ATV.dial ARV @14:16:08.89 ARV.dial :carrier @14:16:09.23 AS.seek 0.5ml @14:16:34.79 AFV.select :carrierSolution @14:16:34.90 delay 2 seconds @14:16:36.93 AS.reconfigure ASconfig @14:16:36.98 AS.seek 1ml @14:17:06.00 delay 1 second @14:17:07.02 AFV.select :holdingCoil @14:17:07.12 delay 1 second @14:17:08.15 ATV.dial :waste @14:17:09.71 AS.reconfigure ASnorm @14:17:09.76 AS.seek :empty @14:18:00.51 AFV.select :carrierSolution @14:18:00.62 delay 2 seconds @14:18:02.65 AS.reconfigure ASconfig @14:18:03.43 AS.seek 0.5ml @14:18:32.51 delay 1 second @14:18:33.53 AFV.select :holdingCoil @14:18:33.63 Building Slug @14:18:33.64 ATV.dial :ARV @14:18:35.18 delay 2 seconds @14:18:37.21 ARV.dial :air1 @14:18:37.61 delay 2 seconds @14:18:39.70 AS.reconfigure ASslow2 @14:18:39.76 AS.seek 0.506ml @14:18:46.16 delay 2 seconds @14:18:48.19 ARV.dial :MM2 @14:18:49.58 delay 2 seconds @14:18:51.61 Adding MM2 @14:18:51.69 AS.seek 0.524ml @14:19:10.18 delay 25 seconds @14:19:35.21 ARV.dial :PP4 @14:19:35.60 Adding PP4 @14:19:35.62 delay 2 seconds @14:19:37.71 AS.seek 0.53ml @14:19:43.99 delay 10 seconds @14:19:54.02 ARV.dial :air3 @14:19:54.40 adding ARV air @14:19:54.42 delay 2 seconds @14:19:56.50 AS.seek 0.536ml @14:20:02.85 delay 5 seconds @14:20:07.88 ARV.dial :carrier @14:20:09.08 delay 7 seconds @14:20:16.18 AS.seek 0.594ml @14:21:14.84 delay 5 seconds @14:21:19.87 ATV.dial :ASV @14:21:20.26 delay 2 seconds @14:21:22.36 AS.seek 0.6ml @14:21:28.68 delay 4 seconds @14:21:32.71 ATV.dial ARV @14:21:33.24 delay 2 seconds @14:21:35.27 ARV.dial :carrier @14:21:35.28 delay 2 seconds @14:21:37.37 AS.seek 0.65ml @14:22:27.91 delay 4 seconds @14:22:31.94 ATV.dial PCR @14:22:32.52 ASV.dial :waste1 @14:22:32.53 ARV.dial :air1 @14:22:32.92 Positioning Slug by 0.324ml @14:22:33.01 AS.seek 0.326ml @14:27:59.21 delay 5 seconds @14:28:04.24 ATV.dial ARV @14:28:04.98 ASV.dial :PCRlock @14:28:05.37 delay 5 seconds @14:28:10.40 Running ML Profile60C350 thermocycling @14:28:10.42 PCR.program Profile60C350 @14:28:10.53 Profile60C350 should complete in < 2:02:45 @14:28:10.54 PCR.run 2:38:26.25 @16:04:22.25 PCR.off @16:04:23.52 PCR for ML completed -- closed /var/log/esp/ML20may2314h325ml.pcr @16:04:23.59 Cleaning PCR @16:04:23.61 AFV.select :carrierSolution @16:04:23.72 delay 2 seconds @16:04:25.75 AS.reconfigure ASconfig @16:04:25.80 AS.seek 0.5ml @16:04:36.14 delay 1 second @16:04:37.16 AFV.select :holdingCoil @16:04:37.26 ASV.dial :waste1 @16:04:37.64 ATV.dial :air3 @16:04:38.87 AS.reconfigure ASslow1 @16:04:38.92 AS.seek 0.51ml @16:04:41.20 delay 2 seconds @16:04:43.22 ATV.dial :bleach @16:04:43.82 AS.seek 0.81ml @16:05:44.44 delay 3 seconds @16:05:47.47 ATV.dial :air3 @16:05:48.00 delay 3 seconds @16:05:51.09 AS.seek 0.835ml @16:05:56.39 delay 3 seconds @16:05:59.42 ATV.dial :bleach @16:06:00.03 AS.seek 0.82ml @16:06:03.35 delay 3 seconds @16:06:06.37 ARV.dial :waste @16:06:06.96 delay 3 seconds @16:06:09.99 ATV.dial :ARV @16:06:10.85 AS.reconfigure ASconfig @16:06:10.90 AS.seek 0.47ml @16:06:31.32 AFV.select :carrierSolution @16:06:31.43 delay 2 seconds @16:06:33.47 AS.seek 1ml @16:07:04.24 delay 1 second @16:07:05.26 AFV.select :holdingCoil @16:07:05.36 delay 1 second @16:07:06.39 AS.reconfigure ASfast @16:07:06.44 AS.seek 0ml @16:07:33.80 AFV.select :carrierSolution @16:07:33.91 delay 2 seconds @16:07:35.94 AS.reconfigure ASconfig @16:07:35.99 AS.seek 1ml @16:08:33.80 delay 1 second @16:08:34.82 AFV.select :holdingCoil @16:08:34.92 delay 1 second @16:08:35.95 AS.reconfigure ASfast @16:08:36.00 AS.seek 0ml @16:09:03.36 AFV.select :carrierSolution @16:09:03.47 delay 2 seconds @16:09:05.50 AS.reconfigure ASconfig @16:09:05.55 AS.seek 1ml @16:10:03.35 delay 1 second @16:10:04.37 AFV.select :holdingCoil @16:10:04.47 delay 1 second @16:10:05.50 AS.reconfigure ASfast @16:10:05.55 AS.seek 0ml @16:10:32.93 ARV.dial between carrier and air1 @16:10:33.41 ATV.dial :air3 @16:10:34.64 AS.reconfigure ASslow1 @16:10:34.69 AS.seek 0.01ml @16:10:36.97 delay 2 seconds @16:10:38.99 ATV.dial :bleach @16:10:39.59 AS.seek 0.81ml @16:13:20.76 delay 3 seconds @16:13:23.79 ATV.dial :air3 @16:13:24.32 delay 3 seconds @16:13:27.41 AS.seek 0.835ml @16:13:32.68 delay 3 seconds @16:13:35.70 ATV.dial :bleach @16:13:36.31 AS.seek 0.82ml @16:13:39.62 delay 3 seconds @16:13:42.65 ATV.dial PCR @16:13:43.04 AS.reconfigure ASconfig @16:13:43.09 AS.seek :empty @16:14:30.56 PCR.program :bleachProfile @16:14:30.62 PCR program should complete in < 2:45 @16:14:30.63 PCR.run 10:00 @16:17:44.57 PCR.off @16:17:44.60 AFV.select :carrierSolution @16:17:44.71 delay 2 seconds @16:17:46.75 AS.seek 1ml @16:18:44.54 delay 1 second @16:18:45.56 AFV.select :holdingCoil @16:18:45.66 delay 1 second @16:18:46.69 AS.reconfigure ASfast @16:18:46.74 AS.seek 0ml @16:19:14.07 delay 3 seconds @16:19:17.10 AFV.select :carrierSolution @16:19:17.20 delay 2 seconds @16:19:19.23 AS.reconfigure ASconfig @16:19:19.29 AS.seek 1ml @16:20:17.09 delay 1 second @16:20:18.11 AFV.select :holdingCoil @16:20:18.21 delay 1 second @16:20:19.24 AS.reconfigure ASfast @16:20:19.29 AS.seek 0ml @16:20:46.67 delay 3 seconds @16:20:49.70 AFV.select :carrierSolution @16:20:49.80 delay 2 seconds @16:20:51.83 AS.reconfigure ASconfig @16:20:51.89 AS.seek 1ml @16:21:49.70 delay 1 second @16:21:50.72 AFV.select :holdingCoil @16:21:50.82 delay 1 second @16:21:51.85 AS.reconfigure ASfast @16:21:51.90 AS.seek 0ml @16:22:19.26 delay 3 seconds @16:22:22.29 AFV.select :carrierSolution @16:22:22.39 delay 2 seconds @16:22:24.42 AS.reconfigure ASconfig @16:22:24.47 AS.seek 1ml @16:23:22.25 delay 1 second @16:23:23.27 AFV.select :holdingCoil @16:23:23.37 delay 1 second @16:23:24.40 AS.reconfigure ASfast @16:23:24.45 AS.seek 0ml @16:23:51.80 delay 3 seconds @16:23:54.83 AFV.select :carrierSolution @16:23:54.93 delay 2 seconds @16:23:56.96 AS.reconfigure ASconfig @16:23:57.02 AS.seek 1ml @16:24:54.81 delay 1 second @16:24:55.83 AFV.select :holdingCoil @16:24:55.93 delay 1 second @16:24:56.96 AS.reconfigure ASnorm @16:24:57.01 AS.seek 0ml @16:25:47.74 Setting up PCR for CG -- writing /var/log/esp/CG20may2316h325ml.pcr @16:25:47.76 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:25:47.77 Priming with carrier @16:25:47.78 ATV.dial ARV @16:25:48.38 ARV.dial :carrier @16:25:48.73 AS.seek 0.5ml @16:26:14.36 AFV.select :carrierSolution @16:26:14.47 delay 2 seconds @16:26:16.50 AS.reconfigure ASconfig @16:26:16.55 AS.seek 1ml @16:26:45.61 delay 1 second @16:26:46.63 AFV.select :holdingCoil @16:26:46.73 delay 1 second @16:26:47.76 ATV.dial :waste @16:26:49.33 AS.reconfigure ASnorm @16:26:49.39 AS.seek :empty @16:27:40.13 AFV.select :carrierSolution @16:27:40.24 delay 2 seconds @16:27:42.27 AS.reconfigure ASconfig @16:27:42.32 AS.seek 0.5ml @16:28:11.42 delay 1 second @16:28:12.44 AFV.select :holdingCoil @16:28:12.54 Building Slug @16:28:12.55 ATV.dial :ARV @16:28:14.10 delay 2 seconds @16:28:16.13 ARV.dial :air1 @16:28:16.53 delay 2 seconds @16:28:18.62 AS.reconfigure ASslow2 @16:28:18.67 AS.seek 0.506ml @16:28:24.96 delay 2 seconds @16:28:26.98 ARV.dial :MM1 @16:28:27.55 delay 2 seconds @16:28:29.58 Adding MM1 @16:28:29.65 AS.seek 0.524ml @16:28:47.88 delay 25 seconds @16:29:12.91 ARV.dial :PP2 @16:29:13.28 Adding PP2 @16:29:13.30 delay 2 seconds @16:29:15.38 AS.seek 0.53ml @16:29:21.69 delay 10 seconds @16:29:31.72 ARV.dial :air2 @16:29:32.10 adding ARV air @16:29:32.12 delay 2 seconds @16:29:34.20 AS.seek 0.536ml @16:29:40.58 delay 5 seconds @16:29:45.61 ARV.dial :carrier @16:29:47.53 delay 7 seconds @16:29:54.62 AS.seek 0.594ml @16:30:53.13 delay 5 seconds @16:30:58.16 ATV.dial :ASV @16:30:58.55 delay 2 seconds @16:31:00.65 AS.seek 0.6ml @16:31:06.86 delay 4 seconds @16:31:10.89 ATV.dial ARV @16:31:11.43 delay 2 seconds @16:31:13.46 ARV.dial :carrier @16:31:13.47 delay 2 seconds @16:31:15.56 AS.seek 0.65ml @16:32:06.07 delay 4 seconds @16:32:10.10 ATV.dial PCR @16:32:10.69 ASV.dial :waste1 @16:32:10.70 ARV.dial :air1 @16:32:11.09 Positioning Slug by 0.324ml @16:32:11.18 AS.seek 0.326ml @16:37:37.04 delay 5 seconds @16:37:42.07 ATV.dial ARV @16:37:42.81 ASV.dial :PCRlock @16:37:43.20 delay 5 seconds @16:37:48.23 Running CG Profile60C300 thermocycling @16:37:48.24 PCR.program Profile60C300 @16:37:48.35 Profile60C300 should complete in < 1:59:25 @16:37:48.36 PCR.run 2:34:16.25 @18:13:17.61 PCR.off @18:13:18.28 PCR for CG completed -- closed /var/log/esp/CG20may2316h325ml.pcr @18:13:18.35 Cleaning PCR @18:13:18.36 AFV.select :carrierSolution @18:13:18.47 delay 2 seconds @18:13:20.50 AS.reconfigure ASconfig @18:13:20.55 AS.seek 0.5ml @18:13:30.86 delay 1 second @18:13:31.88 AFV.select :holdingCoil @18:13:31.98 ASV.dial :waste1 @18:13:32.36 ATV.dial :air3 @18:13:33.58 AS.reconfigure ASslow1 @18:13:33.63 AS.seek 0.51ml @18:13:35.88 delay 2 seconds @18:13:37.90 ATV.dial :bleach @18:13:38.50 AS.seek 0.81ml @18:14:39.08 delay 3 seconds @18:14:42.10 ATV.dial :air3 @18:14:43.37 delay 3 seconds @18:14:46.46 AS.seek 0.835ml @18:14:51.74 delay 3 seconds @18:14:54.77 ATV.dial :bleach @18:14:55.38 AS.seek 0.82ml @18:14:58.64 delay 3 seconds @18:15:01.67 ARV.dial :waste @18:15:02.25 delay 3 seconds @18:15:05.28 ATV.dial :ARV @18:15:06.13 AS.reconfigure ASconfig @18:15:06.18 AS.seek 0.47ml @18:15:26.61 AFV.select :carrierSolution @18:15:26.72 delay 2 seconds @18:15:28.76 AS.seek 1ml @18:15:59.59 delay 1 second @18:16:00.61 AFV.select :holdingCoil @18:16:00.71 delay 1 second @18:16:01.74 AS.reconfigure ASfast @18:16:01.79 AS.seek 0ml @18:16:29.14 AFV.select :carrierSolution @18:16:29.25 delay 2 seconds @18:16:31.28 AS.reconfigure ASconfig @18:16:31.33 AS.seek 1ml @18:17:29.11 delay 1 second @18:17:30.13 AFV.select :holdingCoil @18:17:30.23 delay 1 second @18:17:31.26 AS.reconfigure ASfast @18:17:31.31 AS.seek 0ml @18:17:58.66 AFV.select :carrierSolution @18:17:58.77 delay 2 seconds @18:18:00.80 AS.reconfigure ASconfig @18:18:00.85 AS.seek 1ml @18:18:58.61 delay 1 second @18:18:59.63 AFV.select :holdingCoil @18:18:59.73 delay 1 second @18:19:00.76 AS.reconfigure ASfast @18:19:00.81 AS.seek 0ml @18:19:28.17 ARV.dial between carrier and air1 @18:19:28.64 ATV.dial :air3 @18:19:29.87 AS.reconfigure ASslow1 @18:19:29.92 AS.seek 0.01ml @18:19:32.24 delay 2 seconds @18:19:34.26 ATV.dial :bleach @18:19:34.87 AS.seek 0.81ml @18:22:16.02 delay 3 seconds @18:22:19.04 ATV.dial :air3 @18:22:19.58 delay 3 seconds @18:22:22.67 AS.seek 0.835ml @18:22:27.95 delay 3 seconds @18:22:30.98 ATV.dial :bleach @18:22:31.59 AS.seek 0.82ml @18:22:34.88 delay 3 seconds @18:22:37.91 ATV.dial PCR @18:22:38.30 AS.reconfigure ASconfig @18:22:38.35 AS.seek :empty @18:23:25.82 PCR.program :bleachProfile @18:23:25.87 PCR program should complete in < 2:45 @18:23:25.88 PCR.run 10:00 @18:26:39.81 PCR.off @18:26:39.85 AFV.select :carrierSolution @18:26:39.96 delay 2 seconds @18:26:42.00 AS.seek 1ml @18:27:39.79 delay 1 second @18:27:40.81 AFV.select :holdingCoil @18:27:40.91 delay 1 second @18:27:41.94 AS.reconfigure ASfast @18:27:41.99 AS.seek 0ml @18:28:09.35 delay 3 seconds @18:28:12.38 AFV.select :carrierSolution @18:28:12.48 delay 2 seconds @18:28:14.51 AS.reconfigure ASconfig @18:28:14.56 AS.seek 1ml @18:29:12.32 delay 1 second @18:29:13.34 AFV.select :holdingCoil @18:29:13.44 delay 1 second @18:29:14.47 AS.reconfigure ASfast @18:29:14.52 AS.seek 0ml @18:29:41.88 delay 3 seconds @18:29:44.91 AFV.select :carrierSolution @18:29:45.01 delay 2 seconds @18:29:47.04 AS.reconfigure ASconfig @18:29:47.09 AS.seek 1ml @18:30:44.87 delay 1 second @18:30:45.89 AFV.select :holdingCoil @18:30:45.99 delay 1 second @18:30:47.02 AS.reconfigure ASfast @18:30:47.07 AS.seek 0ml @18:31:14.42 delay 3 seconds @18:31:17.45 AFV.select :carrierSolution @18:31:17.55 delay 2 seconds @18:31:19.58 AS.reconfigure ASconfig @18:31:19.64 AS.seek 1ml @18:32:17.42 delay 1 second @18:32:18.44 AFV.select :holdingCoil @18:32:19.18 delay 1 second @18:32:20.29 AS.reconfigure ASfast @18:32:20.34 AS.seek 0ml @18:32:47.68 delay 3 seconds @18:32:50.71 AFV.select :carrierSolution @18:32:50.81 delay 2 seconds @18:32:52.84 AS.reconfigure ASconfig @18:32:52.90 AS.seek 1ml @18:33:50.65 delay 1 second @18:33:51.67 AFV.select :holdingCoil @18:33:51.77 delay 1 second @18:33:52.80 AS.reconfigure ASnorm @18:33:52.85 AS.seek 0ml @18:34:43.58 Setting up PCR for NM -- writing /var/log/esp/NM20may2318h325ml.pcr @18:34:43.60 Building PCR Slug from ASV with MM1 mix, PP1 primer @18:34:43.61 Priming with carrier @18:34:43.62 ATV.dial ARV @18:34:44.21 ARV.dial :carrier @18:34:44.55 AS.seek 0.5ml @18:35:10.14 AFV.select :carrierSolution @18:35:10.25 delay 2 seconds @18:35:12.28 AS.reconfigure ASconfig @18:35:12.33 AS.seek 1ml @18:35:41.32 delay 1 second @18:35:42.34 AFV.select :holdingCoil @18:35:42.44 delay 1 second @18:35:43.47 ATV.dial :waste @18:35:45.06 AS.reconfigure ASnorm @18:35:45.12 AS.seek :empty @18:36:35.86 AFV.select :carrierSolution @18:36:35.97 delay 2 seconds @18:36:38.00 AS.reconfigure ASconfig @18:36:38.05 AS.seek 0.5ml @18:37:07.15 delay 1 second @18:37:08.17 AFV.select :holdingCoil @18:37:08.27 Building Slug @18:37:08.28 ATV.dial :ARV @18:37:09.83 delay 2 seconds @18:37:11.86 ARV.dial :air1 @18:37:12.26 delay 2 seconds @18:37:14.35 AS.reconfigure ASslow2 @18:37:14.40 AS.seek 0.506ml @18:37:20.61 delay 2 seconds @18:37:22.64 ARV.dial :MM1 @18:37:23.22 delay 2 seconds @18:37:25.25 Adding MM1 @18:37:25.32 AS.seek 0.524ml @18:37:43.69 delay 25 seconds @18:38:08.72 ARV.dial :PP1 @18:38:09.11 Adding PP1 @18:38:09.13 delay 2 seconds @18:38:11.21 AS.seek 0.53ml @18:38:17.80 delay 10 seconds @18:38:27.83 ARV.dial :air1 @18:38:28.20 adding ARV air @18:38:28.22 delay 2 seconds @18:38:30.30 AS.seek 0.536ml @18:38:36.75 delay 5 seconds @18:38:41.78 ARV.dial :carrier @18:38:42.17 delay 7 seconds @18:38:49.26 AS.seek 0.594ml @18:39:47.81 delay 5 seconds @18:39:52.84 ATV.dial :ASV @18:39:53.23 delay 2 seconds @18:39:55.32 AS.seek 0.6ml @18:40:01.66 delay 4 seconds @18:40:05.69 ATV.dial ARV @18:40:06.23 delay 2 seconds @18:40:08.26 ARV.dial :carrier @18:40:08.27 delay 2 seconds @18:40:10.36 AS.seek 0.65ml @18:41:01.27 delay 4 seconds @18:41:05.30 ATV.dial PCR @18:41:05.89 ASV.dial :waste1 @18:41:05.90 ARV.dial :air1 @18:41:06.29 Positioning Slug by 0.324ml @18:41:06.38 AS.seek 0.326ml @18:46:32.57 delay 5 seconds @18:46:37.60 ATV.dial ARV @18:46:38.33 ASV.dial :PCRlock @18:46:38.71 delay 5 seconds @18:46:43.74 Running NM Profile60C300 thermocycling @18:46:43.76 PCR.program Profile60C300 @18:46:43.86 Profile60C300 should complete in < 1:59:25 @18:46:43.87 PCR.run 2:34:16.25 @20:22:14.20 PCR.off @20:22:14.86 PCR for NM completed -- closed /var/log/esp/NM20may2318h325ml.pcr @20:22:14.93 Cleaning PCR @20:22:14.94 AFV.select :carrierSolution @20:22:15.05 delay 2 seconds @20:22:17.08 AS.reconfigure ASconfig @20:22:17.13 AS.seek 0.5ml @20:22:27.43 delay 1 second @20:22:28.45 AFV.select :holdingCoil @20:22:28.55 ASV.dial :waste1 @20:22:28.93 ATV.dial :air3 @20:22:30.15 AS.reconfigure ASslow1 @20:22:30.20 AS.seek 0.51ml @20:22:32.45 delay 2 seconds @20:22:34.48 ATV.dial :bleach @20:22:35.09 AS.seek 0.81ml @20:23:35.69 delay 3 seconds @20:23:38.72 ATV.dial :air3 @20:23:39.26 delay 3 seconds @20:23:42.35 AS.seek 0.835ml @20:23:47.62 delay 3 seconds @20:23:50.65 ATV.dial :bleach @20:23:51.25 AS.seek 0.82ml @20:23:54.54 delay 3 seconds @20:23:57.57 ARV.dial :waste @20:23:58.15 delay 3 seconds @20:24:01.18 ATV.dial :ARV @20:24:02.03 AS.reconfigure ASconfig @20:24:02.08 AS.seek 0.47ml @20:24:22.50 AFV.select :carrierSolution @20:24:22.62 delay 2 seconds @20:24:24.66 AS.seek 1ml @20:24:55.41 delay 1 second @20:24:56.43 AFV.select :holdingCoil @20:24:56.53 delay 1 second @20:24:57.56 AS.reconfigure ASfast @20:24:57.61 AS.seek 0ml @20:25:24.93 AFV.select :carrierSolution @20:25:25.04 delay 2 seconds @20:25:27.07 AS.reconfigure ASconfig @20:25:27.12 AS.seek 1ml @20:26:24.88 delay 1 second @20:26:25.90 AFV.select :holdingCoil @20:26:26.00 delay 1 second @20:26:27.03 AS.reconfigure ASfast @20:26:27.08 AS.seek 0ml @20:26:54.42 AFV.select :carrierSolution @20:26:54.53 delay 2 seconds @20:26:56.56 AS.reconfigure ASconfig @20:26:56.61 AS.seek 1ml @20:27:54.40 delay 1 second @20:27:55.42 AFV.select :holdingCoil @20:27:55.52 delay 1 second @20:27:56.55 AS.reconfigure ASfast @20:27:56.60 AS.seek 0ml @20:28:23.96 ARV.dial between carrier and air1 @20:28:24.43 ATV.dial :air3 @20:28:25.66 AS.reconfigure ASslow1 @20:28:25.71 AS.seek 0.01ml @20:28:28.06 delay 2 seconds @20:28:30.08 ATV.dial :bleach @20:28:31.42 AS.seek 0.81ml @20:31:12.57 delay 3 seconds @20:31:15.60 ATV.dial :air3 @20:31:16.14 delay 3 seconds @20:31:19.23 AS.seek 0.835ml @20:31:24.51 delay 3 seconds @20:31:27.54 ATV.dial :bleach @20:31:28.15 AS.seek 0.82ml @20:31:31.45 delay 3 seconds @20:31:34.48 ATV.dial PCR @20:31:34.87 AS.reconfigure ASconfig @20:31:34.92 AS.seek :empty @20:32:22.39 PCR.program :bleachProfile @20:32:22.45 PCR program should complete in < 2:45 @20:32:22.46 PCR.run 10:00 @20:35:36.41 PCR.off @20:35:36.44 AFV.select :carrierSolution @20:35:36.55 delay 2 seconds @20:35:38.59 AS.seek 1ml @20:36:36.42 delay 1 second @20:36:37.44 AFV.select :holdingCoil @20:36:37.54 delay 1 second @20:36:38.57 AS.reconfigure ASfast @20:36:38.62 AS.seek 0ml @20:37:05.95 delay 3 seconds @20:37:08.98 AFV.select :carrierSolution @20:37:09.08 delay 2 seconds @20:37:11.11 AS.reconfigure ASconfig @20:37:11.18 AS.seek 1ml @20:38:08.95 delay 1 second @20:38:09.97 AFV.select :holdingCoil @20:38:10.07 delay 1 second @20:38:11.10 AS.reconfigure ASfast @20:38:11.15 AS.seek 0ml @20:38:38.49 delay 3 seconds @20:38:41.52 AFV.select :carrierSolution @20:38:41.62 delay 2 seconds @20:38:43.65 AS.reconfigure ASconfig @20:38:43.71 AS.seek 1ml @20:39:41.53 delay 1 second @20:39:42.55 AFV.select :holdingCoil @20:39:42.65 delay 1 second @20:39:43.68 AS.reconfigure ASfast @20:39:43.73 AS.seek 0ml @20:40:11.08 delay 3 seconds @20:40:14.11 AFV.select :carrierSolution @20:40:14.21 delay 2 seconds @20:40:16.24 AS.reconfigure ASconfig @20:40:16.30 AS.seek 1ml @20:41:14.08 delay 1 second @20:41:15.10 AFV.select :holdingCoil @20:41:15.20 delay 1 second @20:41:16.23 AS.reconfigure ASfast @20:41:16.28 AS.seek 0ml @20:41:43.61 delay 3 seconds @20:41:46.64 AFV.select :carrierSolution @20:41:46.74 delay 2 seconds @20:41:48.77 AS.reconfigure ASconfig @20:41:48.83 AS.seek 1ml @20:42:46.60 delay 1 second @20:42:47.62 AFV.select :holdingCoil @20:42:47.72 delay 1 second @20:42:48.75 AS.reconfigure ASnorm @20:42:48.80 AS.seek 0ml @20:43:39.53 Setting up PCR for IPc -- writing /var/log/esp/IPc20may2320h325ml.pcr @20:43:39.55 Building PCR Slug from ASV with MM3 mix, PP6 primer @20:43:39.56 Priming with carrier @20:43:39.57 ATV.dial ARV @20:43:40.16 ARV.dial :carrier @20:43:40.50 AS.seek 0.5ml @20:44:06.11 AFV.select :carrierSolution @20:44:06.22 delay 2 seconds @20:44:08.25 AS.reconfigure ASconfig @20:44:08.30 AS.seek 1ml @20:44:37.30 delay 1 second @20:44:38.32 AFV.select :holdingCoil @20:44:38.42 delay 1 second @20:44:39.45 ATV.dial :waste @20:44:41.01 AS.reconfigure ASnorm @20:44:41.06 AS.seek :empty @20:45:31.80 AFV.select :carrierSolution @20:45:31.91 delay 2 seconds @20:45:33.94 AS.reconfigure ASconfig @20:45:33.99 AS.seek 0.5ml @20:46:03.08 delay 1 second @20:46:04.10 AFV.select :holdingCoil @20:46:04.20 Building Slug @20:46:04.21 ATV.dial :ARV @20:46:05.76 delay 2 seconds @20:46:07.79 ARV.dial :air1 @20:46:08.19 delay 2 seconds @20:46:10.28 AS.reconfigure ASslow2 @20:46:10.33 AS.seek 0.506ml @20:46:16.55 delay 2 seconds @20:46:18.57 ARV.dial :MM3 @20:46:19.57 delay 2 seconds @20:46:22.33 Adding MM3 @20:46:22.41 AS.seek 0.524ml @20:46:40.76 delay 25 seconds @20:47:05.79 ARV.dial :PP6 @20:47:06.18 Adding PP6 @20:47:06.20 delay 2 seconds @20:47:08.28 AS.seek 0.53ml @20:47:14.71 delay 10 seconds @20:47:24.74 ARV.dial :air1 @20:47:25.52 adding ARV air @20:47:25.54 delay 2 seconds @20:47:27.62 AS.seek 0.536ml @20:47:33.84 delay 5 seconds @20:47:38.87 ARV.dial :carrier @20:47:39.26 delay 7 seconds @20:47:46.35 AS.seek 0.594ml @20:48:44.84 delay 5 seconds @20:48:49.88 ATV.dial :ASV @20:48:50.29 delay 2 seconds @20:48:52.38 AS.seek 0.6ml @20:48:58.70 delay 4 seconds @20:49:02.73 ATV.dial ARV @20:49:03.26 delay 2 seconds @20:49:05.29 ARV.dial :carrier @20:49:05.30 delay 2 seconds @20:49:07.39 AS.seek 0.65ml @20:49:57.81 delay 4 seconds @20:50:01.84 ATV.dial PCR @20:50:02.43 ASV.dial :waste1 @20:50:02.44 ARV.dial :air1 @20:50:02.85 Positioning Slug by 0.324ml @20:50:02.94 AS.seek 0.326ml @20:55:28.98 delay 5 seconds @20:55:34.01 ATV.dial ARV @20:55:34.75 ASV.dial :PCRlock @20:55:35.14 delay 5 seconds @20:55:40.16 Running IPc ProfileIPc thermocycling @20:55:40.18 PCR.program ProfileIPc @20:55:40.28 ProfileIPc should complete in < 1:36:15 @20:55:40.29 PCR.run 2:05:18.75 @22:13:46.80 PCR.off @22:13:47.36 PCR for IPc completed -- closed /var/log/esp/IPc20may2320h325ml.pcr @22:13:47.43 Cleaning PCR @22:13:47.44 AFV.select :carrierSolution @22:13:47.55 delay 2 seconds @22:13:49.58 AS.reconfigure ASconfig @22:13:49.63 AS.seek 0.5ml @22:13:59.94 delay 1 second @22:14:00.96 AFV.select :holdingCoil @22:14:01.06 ASV.dial :waste1 @22:14:01.44 ATV.dial :air3 @22:14:02.84 AS.reconfigure ASslow1 @22:14:02.89 AS.seek 0.51ml @22:14:05.18 delay 2 seconds @22:14:07.21 ATV.dial :bleach @22:14:07.82 AS.seek 0.81ml @22:15:08.42 delay 3 seconds @22:15:11.45 ATV.dial :air3 @22:15:11.99 delay 3 seconds @22:15:15.08 AS.seek 0.835ml @22:15:20.39 delay 3 seconds @22:15:23.41 ATV.dial :bleach @22:15:24.01 AS.seek 0.82ml @22:15:27.33 delay 3 seconds @22:15:30.35 ARV.dial :waste @22:15:30.94 delay 3 seconds @22:15:33.97 ATV.dial :ARV @22:15:34.82 AS.reconfigure ASconfig @22:15:34.87 AS.seek 0.47ml @22:15:55.27 AFV.select :carrierSolution @22:15:55.39 delay 2 seconds @22:15:57.43 AS.seek 1ml @22:16:28.21 delay 1 second @22:16:29.23 AFV.select :holdingCoil @22:16:29.33 delay 1 second @22:16:30.36 AS.reconfigure ASfast @22:16:30.41 AS.seek 0ml @22:16:57.74 AFV.select :carrierSolution @22:16:57.85 delay 2 seconds @22:16:59.88 AS.reconfigure ASconfig @22:16:59.93 AS.seek 1ml @22:17:57.71 delay 1 second @22:17:58.73 AFV.select :holdingCoil @22:17:58.83 delay 1 second @22:17:59.86 AS.reconfigure ASfast @22:17:59.91 AS.seek 0ml @22:18:27.26 AFV.select :carrierSolution @22:18:27.37 delay 2 seconds @22:18:29.40 AS.reconfigure ASconfig @22:18:29.45 AS.seek 1ml @22:19:27.24 delay 1 second @22:19:28.26 AFV.select :holdingCoil @22:19:28.36 delay 1 second @22:19:29.39 AS.reconfigure ASfast @22:19:29.44 AS.seek 0ml @22:19:56.77 ARV.dial between carrier and air1 @22:19:57.26 ATV.dial :air3 @22:19:58.48 AS.reconfigure ASslow1 @22:19:58.53 AS.seek 0.01ml @22:20:00.85 delay 2 seconds @22:20:02.87 ATV.dial :bleach @22:20:03.48 AS.seek 0.81ml @22:22:44.60 delay 3 seconds @22:22:47.63 ATV.dial :air3 @22:22:48.16 delay 3 seconds @22:22:51.25 AS.seek 0.835ml @22:22:56.57 delay 3 seconds @22:22:59.59 ATV.dial :bleach @22:23:00.20 AS.seek 0.82ml @22:23:03.43 delay 3 seconds @22:23:06.46 ATV.dial PCR @22:23:06.85 AS.reconfigure ASconfig @22:23:06.90 AS.seek :empty @22:23:54.35 PCR.program :bleachProfile @22:23:54.40 PCR program should complete in < 2:45 @22:23:54.41 PCR.run 10:00 @22:27:09.06 PCR.off @22:27:09.09 AFV.select :carrierSolution @22:27:09.20 delay 2 seconds @22:27:11.24 AS.seek 1ml @22:28:09.06 delay 1 second @22:28:10.08 AFV.select :holdingCoil @22:28:10.18 delay 1 second @22:28:11.21 AS.reconfigure ASfast @22:28:11.26 AS.seek 0ml @22:28:38.61 delay 3 seconds @22:28:41.64 AFV.select :carrierSolution @22:28:41.74 delay 2 seconds @22:28:43.77 AS.reconfigure ASconfig @22:28:43.83 AS.seek 1ml @22:29:41.61 delay 1 second @22:29:42.63 AFV.select :holdingCoil @22:29:42.73 delay 1 second @22:29:43.76 AS.reconfigure ASfast @22:29:43.81 AS.seek 0ml @22:30:11.15 delay 3 seconds @22:30:14.18 AFV.select :carrierSolution @22:30:14.28 delay 2 seconds @22:30:16.31 AS.reconfigure ASconfig @22:30:16.37 AS.seek 1ml @22:31:14.14 delay 1 second @22:31:15.16 AFV.select :holdingCoil @22:31:15.26 delay 1 second @22:31:16.29 AS.reconfigure ASfast @22:31:16.34 AS.seek 0ml @22:31:43.70 delay 3 seconds @22:31:46.73 AFV.select :carrierSolution @22:31:46.83 delay 2 seconds @22:31:48.86 AS.reconfigure ASconfig @22:31:48.92 AS.seek 1ml @22:32:46.73 delay 1 second @22:32:47.75 AFV.select :holdingCoil @22:32:47.85 delay 1 second @22:32:48.88 AS.reconfigure ASfast @22:32:48.93 AS.seek 0ml @22:33:16.26 delay 3 seconds @22:33:19.29 AFV.select :carrierSolution @22:33:19.39 delay 2 seconds @22:33:21.42 AS.reconfigure ASconfig @22:33:21.47 AS.seek 1ml @22:34:19.25 delay 1 second @22:34:20.27 AFV.select :holdingCoil @22:34:20.37 delay 1 second @22:34:21.40 AS.reconfigure ASnorm @22:34:21.45 AS.seek 0ml @22:35:12.18 Setting up PCR for IPc2 -- writing /var/log/esp/IPc220may2322h325ml.pcr @22:35:12.20 Building PCR Slug from ASV with MM2 mix, PP3 primer @22:35:12.21 Priming with carrier @22:35:12.22 ATV.dial ARV @22:35:12.81 ARV.dial :carrier @22:35:13.15 AS.seek 0.5ml @22:35:38.72 AFV.select :carrierSolution @22:35:38.84 delay 2 seconds @22:35:40.87 AS.reconfigure ASconfig @22:35:40.92 AS.seek 1ml @22:36:09.94 delay 1 second @22:36:10.96 AFV.select :holdingCoil @22:36:11.06 delay 1 second @22:36:12.09 ATV.dial :waste @22:36:13.66 AS.reconfigure ASnorm @22:36:13.71 AS.seek :empty @22:37:04.45 AFV.select :carrierSolution @22:37:04.56 delay 2 seconds @22:37:06.59 AS.reconfigure ASconfig @22:37:06.64 AS.seek 0.5ml @22:37:35.72 delay 1 second @22:37:36.74 AFV.select :holdingCoil @22:37:36.84 Building Slug @22:37:36.85 ATV.dial :ARV @22:37:38.39 delay 2 seconds @22:37:40.42 ARV.dial :air1 @22:37:40.81 delay 2 seconds @22:37:42.90 AS.reconfigure ASslow2 @22:37:42.95 AS.seek 0.506ml @22:37:49.20 delay 2 seconds @22:37:51.22 ARV.dial :MM2 @22:37:52.61 delay 2 seconds @22:37:54.64 Adding MM2 @22:37:54.71 AS.seek 0.524ml @22:38:13.02 delay 25 seconds @22:38:38.05 ARV.dial :PP3 @22:38:38.42 Adding PP3 @22:38:38.44 delay 2 seconds @22:38:40.52 AS.seek 0.53ml @22:38:46.75 delay 10 seconds @22:38:56.78 ARV.dial :air2 @22:38:57.16 adding ARV air @22:38:57.17 delay 2 seconds @22:38:59.26 AS.seek 0.536ml @22:39:05.47 delay 5 seconds @22:39:10.50 ARV.dial :carrier @22:39:11.71 delay 7 seconds @22:39:18.80 AS.seek 0.594ml @22:40:17.43 delay 5 seconds @22:40:22.46 ATV.dial :ASV @22:40:22.85 delay 2 seconds @22:40:24.94 AS.seek 0.6ml @22:40:32.02 delay 4 seconds @22:40:36.05 ATV.dial ARV @22:40:36.58 delay 2 seconds @22:40:38.61 ARV.dial :carrier @22:40:38.62 delay 2 seconds @22:40:40.71 AS.seek 0.65ml @22:41:31.22 delay 4 seconds @22:41:35.25 ATV.dial PCR @22:41:35.83 ASV.dial :waste1 @22:41:35.84 ARV.dial :air1 @22:41:36.23 Positioning Slug by 0.324ml @22:41:36.32 AS.seek 0.326ml @22:47:02.40 delay 5 seconds @22:47:07.43 ATV.dial ARV @22:47:08.15 ASV.dial :PCRlock @22:47:08.54 delay 5 seconds @22:47:13.57 Running IPc2 ProfileIPc thermocycling @22:47:13.58 PCR.program ProfileIPc @22:47:13.69 ProfileIPc should complete in < 1:36:15 @22:47:13.70 PCR.run 2:05:18.75 @00:05:13.02CEST24-May-20 PCR.off @00:05:13.57 PCR for IPc2 completed -- closed /var/log/esp/IPc220may2322h325ml.pcr @00:05:13.59 Full MFB Decontamination @00:05:13.60 Decon extraction column and decon AIV @00:05:13.62 SPE.hold 2:46:40,55C @00:05:13.67 ASV.dial :SPEdelivery @00:05:14.32 delay 20 seconds @00:05:34.35 AFV.select :carrierSolution @00:05:34.45 delay 2 seconds @00:05:36.48 AS.reconfigure ASconfig @00:05:36.53 AS.seek 0.5ml @00:05:47.59 delay 1 second @00:05:48.61 AFV.select :holdingCoil @00:05:48.74 ATV.dial :air1 @00:05:50.79 AS.reconfigure ASslow1 @00:05:50.84 AS.seek 0.52ml @00:05:55.14 ATV.dial :ASV @00:05:56.72 ASV.dial :SPErecovery @00:05:57.40 AS.seek 0.62ml @00:06:17.79 ATV.dial :waste @00:06:19.79 AS.reconfigure ASnorm @00:06:19.85 AS.seek :empty @00:06:51.45 AFV.select :holdingCoil @00:06:51.46 ATV.dial :waste @00:06:51.48 AS.reconfigure ASconfig @00:06:51.53 AS.seek 0ml @00:06:51.83 AFV.select :carrierSolution @00:06:51.94 delay 2 seconds @00:06:53.98 AS.seek 0.5ml @00:07:23.00 delay 1 second @00:07:24.02 AFV.select :holdingCoil @00:07:24.12 ATV.dial :air3 @00:07:24.83 AS.reconfigure ASslow1 @00:07:24.88 AS.seek 0.52ml @00:07:29.19 ATV.dial :air3 @00:07:29.28 AS.seek 0.53ml @00:07:31.58 delay 2 seconds @00:07:33.60 ATV.dial :bleach @00:07:34.08 AS.seek 0.71ml @00:08:10.59 delay 3 seconds @00:08:13.62 ATV.dial :air3 @00:08:14.20 delay 3 seconds @00:08:17.29 AS.seek 0.735ml @00:08:22.58 delay 3 seconds @00:08:25.61 ATV.dial :bleach @00:08:26.24 AS.seek 0.72ml @00:08:29.55 delay 3 seconds @00:08:32.58 ATV.dial ASV @00:08:33.67 ASV.dial :SPErecovery @00:08:33.76 AS.seek 0.365ml @00:09:45.34 ATV.dial :waste @00:09:47.03 AS.reconfigure ASnorm @00:09:47.08 AS.seek :empty @00:10:05.84 ATV.dial :air3 @00:10:06.52 AS.reconfigure ASslow1 @00:10:06.57 AS.seek 0.009ml @00:10:08.91 delay 2 seconds @00:10:10.93 ATV.dial :bleach @00:10:11.41 AS.seek 0.579ml @00:12:06.33 delay 3 seconds @00:12:09.36 ATV.dial :air3 @00:12:09.89 delay 3 seconds @00:12:12.98 AS.seek 0.604ml @00:12:18.31 delay 3 seconds @00:12:21.34 ATV.dial :bleach @00:12:22.06 AS.seek 0.589ml @00:12:25.36 delay 3 seconds @00:12:28.39 ATV.dial :ASV @00:12:29.41 ASV.dial :wasteR @00:12:29.81 AS.seek 0ml @00:14:28.53 delay 1:00 @00:15:28.56 ATV.dial ASV @00:15:28.57 AFV.select :carrierSolution @00:15:28.67 delay 2 seconds @00:15:30.70 AS.reconfigure ASconfig @00:15:30.75 AS.seek 1ml @00:16:28.53 delay 1 second @00:16:29.55 AFV.select :holdingCoil @00:16:29.65 delay 1 second @00:16:30.68 ASV.dial :wasteR @00:16:30.70 AS.reconfigure ASslow1 @00:16:30.74 AS.seek 0ml @00:19:52.13 delay 10 seconds @00:20:02.16 ATV.dial :air3 @00:20:03.62 AS.seek 0.01ml @00:20:05.91 delay 2 seconds @00:20:07.93 ATV.dial :bleach @00:20:08.53 AS.seek 0.58ml @00:22:03.40 delay 3 seconds @00:22:06.43 ATV.dial :air3 @00:22:06.96 delay 3 seconds @00:22:10.05 AS.seek 0.605ml @00:22:15.36 delay 3 seconds @00:22:18.38 ATV.dial :bleach @00:22:19.78 AS.seek 0.59ml @00:22:23.07 delay 3 seconds @00:22:26.10 ATV.dial ASV @00:22:27.10 ASV.dial :SPEdelivery @00:22:27.49 AS.seek 0ml @00:24:26.40 delay 1:00 @00:25:26.43 AFV.select :carrierSolution @00:25:26.53 delay 2 seconds @00:25:28.56 AS.reconfigure ASconfig @00:25:28.61 AS.seek 1ml @00:26:26.37 delay 1 second @00:26:27.39 AFV.select :holdingCoil @00:26:27.49 delay 1 second @00:26:28.52 ATV.dial ASV @00:26:28.53 ASV.dial :SPEdelivery @00:26:28.55 AS.reconfigure ASslow1 @00:26:28.60 AS.seek 0ml @00:29:49.98 delay 10 seconds @00:30:00.01 ATV.dial :air2 @00:30:00.82 AS.reconfigure ASslow0 @00:30:00.87 AS.seek 0.8ml @00:31:21.67 delay 3 seconds @00:31:24.70 ATV.dial :ASV @00:31:25.64 ASV.dial :SPEdelivery @00:31:25.66 AS.reconfigure ASslow1 @00:31:25.71 AS.seek 0.03ml @00:34:00.84 delay 20 seconds @00:34:20.87 ATV.dial :waste,via: :air3 @00:34:22.53 AS.reconfigure ASconfig @00:34:22.58 AS.seek :empty @00:34:24.64 SPE.coast @00:34:24.74 Removing Bleach from Mixing Coil @00:34:24.75 AFV.select :carrierSolution @00:34:24.85 delay 2 seconds @00:34:26.89 AS.seek 0.5ml @00:34:56.01 delay 1 second @00:34:57.03 AFV.select :holdingCoil @00:34:57.13 ATV.dial :ASV @00:34:58.73 ASV.dial :SPErecovery @00:34:59.37 AS.reconfigure ASslow1 @00:34:59.42 AS.seek 0.73ml @00:35:45.94 ASV.dial :wasteD @00:35:46.50 AS.reconfigure ASnorm @00:35:46.56 AS.seek 0ml @00:36:23.69 AFV.select :carrierSolution @00:36:23.80 delay 2 seconds @00:36:25.83 AS.reconfigure ASconfig @00:36:25.88 AS.seek 0.5ml @00:36:54.91 delay 1 second @00:36:55.93 AFV.select :holdingCoil @00:36:56.03 ATV.dial ASV @00:36:56.04 ASV.dial :SPErecovery @00:36:56.68 AS.reconfigure ASslow0 @00:36:56.73 AS.seek 0.3ml @00:37:17.16 ATV.dial :air2 @00:37:18.16 AS.reconfigure ASslow1 @00:37:18.21 AS.seek 0.32ml @00:37:22.55 ATV.dial ASV @00:37:23.55 AS.reconfigure ASslow0 @00:37:23.60 AS.seek 0.57ml @00:37:49.13 ASV.dial :wasteD @00:37:49.80 AS.reconfigure ASnorm @00:37:49.85 AS.seek 0ml @00:38:18.92 AFV.select :carrierSolution @00:38:19.03 delay 2 seconds @00:38:21.06 AS.reconfigure ASconfig @00:38:21.11 AS.seek 0.5ml @00:38:50.16 delay 1 second @00:38:51.18 AFV.select :holdingCoil @00:38:51.28 ATV.dial ASV @00:38:51.29 ASV.dial :SPErecovery @00:38:51.91 AS.reconfigure ASslow0 @00:38:51.96 AS.seek 0.3ml @00:39:12.38 ATV.dial :air2 @00:39:13.38 AS.reconfigure ASslow1 @00:39:13.43 AS.seek 0.32ml @00:39:17.78 ATV.dial ASV @00:39:18.77 AS.reconfigure ASslow0 @00:39:18.82 AS.seek 0.57ml @00:39:44.30 ASV.dial :wasteD @00:39:44.97 AS.reconfigure ASnorm @00:39:45.02 AS.seek 0ml @00:40:14.10 ATV.dial :air2 @00:40:15.03 AS.reconfigure ASslow0 @00:40:15.09 AS.seek 0.3ml @00:40:45.65 ATV.dial :ASV @00:40:46.63 ASV.dial :wasteD @00:40:46.65 AS.reconfigure ASconfig @00:40:46.69 AS.seek 0.05ml @00:41:01.36 ATV.dial :waste @00:41:03.87 AS.seek 0ml @00:41:07.06 ASV.dial :SPEdelivery @00:41:08.00 deconLysateINT @00:41:08.01 ATV.dial :air3 @00:41:08.67 AS.reconfigure ASslow1 @00:41:08.72 AS.seek 0.01ml @00:41:11.06 delay 2 seconds @00:41:13.08 ATV.dial :bleach @00:41:13.53 AS.seek 0.96ml @00:44:24.83 delay 3 seconds @00:44:27.86 ATV.dial :air3 @00:44:28.38 delay 3 seconds @00:44:31.47 AS.seek 0.985ml @00:44:36.76 delay 3 seconds @00:44:39.79 ATV.dial :bleach @00:44:40.39 AS.seek 0.97ml @00:44:43.69 delay 3 seconds @00:44:46.72 ATV.dial AIV @00:44:47.91 AIV.dial :coilToMFB @00:44:47.93 AWV.select :waste @00:44:47.94 AFV.select :carrierSolution @00:44:48.05 delay 2 seconds @00:44:50.08 AS.reconfigure ASconfig @00:44:50.13 AS.seek 1ml @00:44:52.16 delay 1 second @00:44:53.18 AFV.select :holdingCoil @00:44:53.28 delay 1 second @00:44:54.31 AS.reconfigure ASslow0 @00:44:54.36 AS.seek :empty @00:46:35.32 AFV.select :carrierSolution @00:46:35.43 delay 2 seconds @00:46:37.46 AS.reconfigure ASconfig @00:46:37.51 AS.seek 1ml @00:47:35.32 delay 1 second @00:47:36.34 AFV.select :holdingCoil @00:47:36.44 delay 1 second @00:47:37.47 AS.reconfigure ASslow0 @00:47:37.52 AS.seek :empty @00:49:18.49 AFV.select :carrierSolution @00:49:18.60 delay 2 seconds @00:49:20.63 AS.reconfigure ASconfig @00:49:20.68 AS.seek 1ml @00:50:18.49 delay 1 second @00:50:19.51 AFV.select :holdingCoil @00:50:19.61 delay 1 second @00:50:20.64 AS.reconfigure ASslow0 @00:50:20.69 AS.seek :empty @00:52:01.62 ATV.dial :air2 @00:52:02.28 AS.reconfigure ASslow1 @00:52:02.33 AS.seek 0.009ml @00:52:04.66 AS.reconfigure ASslow0 @00:52:04.71 AS.seek 1ml @00:53:44.67 ATV.dial AIV @00:53:45.41 AS.seek 0.02ml @00:55:24.29 ATV.dial :waste @00:55:25.85 AS.seek 0ml @00:55:28.15 AWV.select :air @00:55:28.32 Cleaning PCR @00:55:28.33 AFV.select :carrierSolution @00:55:28.43 delay 2 seconds @00:55:30.46 AS.reconfigure ASconfig @00:55:30.51 AS.seek 0.5ml @00:55:59.54 delay 1 second @00:56:00.56 AFV.select :holdingCoil @00:56:00.66 ASV.dial :waste1 @00:56:01.03 ATV.dial :air3 @00:56:01.69 AS.reconfigure ASslow1 @00:56:01.74 AS.seek 0.51ml @00:56:04.01 delay 2 seconds @00:56:06.03 ATV.dial :bleach @00:56:06.49 AS.seek 0.81ml @00:57:07.06 delay 3 seconds @00:57:10.09 ATV.dial :air3 @00:57:10.62 delay 3 seconds @00:57:13.71 AS.seek 0.835ml @00:57:19.08 delay 3 seconds @00:57:22.11 ATV.dial :bleach @00:57:22.72 AS.seek 0.82ml @00:57:25.98 delay 3 seconds @00:57:29.01 ARV.dial :waste @00:57:29.59 delay 3 seconds @00:57:32.62 ATV.dial :ARV @00:57:33.47 AS.reconfigure ASconfig @00:57:33.52 AS.seek 0.47ml @00:57:53.93 AFV.select :carrierSolution @00:57:54.04 delay 2 seconds @00:57:56.08 AS.seek 1ml @00:58:26.87 delay 1 second @00:58:27.89 AFV.select :holdingCoil @00:58:27.99 delay 1 second @00:58:29.02 AS.reconfigure ASfast @00:58:29.07 AS.seek 0ml @00:58:56.42 AFV.select :carrierSolution @00:58:56.53 delay 2 seconds @00:58:59.26 AS.reconfigure ASconfig @00:58:59.33 AS.seek 1ml @00:59:57.09 delay 1 second @00:59:58.11 AFV.select :holdingCoil @00:59:58.21 delay 1 second @00:59:59.24 AS.reconfigure ASfast @00:59:59.29 AS.seek 0ml @01:00:26.65 AFV.select :carrierSolution @01:00:26.76 delay 2 seconds @01:00:28.79 AS.reconfigure ASconfig @01:00:28.84 AS.seek 1ml @01:01:26.64 delay 1 second @01:01:27.66 AFV.select :holdingCoil @01:01:27.76 delay 1 second @01:01:28.79 AS.reconfigure ASfast @01:01:28.84 AS.seek 0ml @01:01:56.20 ARV.dial between carrier and air1 @01:01:56.69 ATV.dial :air3 @01:01:57.93 AS.reconfigure ASslow1 @01:01:57.98 AS.seek 0.01ml @01:02:00.33 delay 2 seconds @01:02:02.36 ATV.dial :bleach @01:02:02.97 AS.seek 0.81ml @01:04:44.11 delay 3 seconds @01:04:47.14 ATV.dial :air3 @01:04:47.67 delay 3 seconds @01:04:50.76 AS.seek 0.835ml @01:04:56.12 delay 3 seconds @01:04:59.15 ATV.dial :bleach @01:04:59.76 AS.seek 0.82ml @01:05:03.08 delay 3 seconds @01:05:06.11 ATV.dial PCR @01:05:06.50 AS.reconfigure ASconfig @01:05:06.55 AS.seek :empty @01:05:54.02 PCR.program :bleachProfile @01:05:54.08 PCR program should complete in < 2:45 @01:05:54.09 PCR.run 10:00 @01:09:20.15 PCR.off @01:09:20.17 AFV.select :carrierSolution @01:09:20.28 delay 2 seconds @01:09:22.32 AS.seek 1ml @01:10:20.19 delay 1 second @01:10:21.21 AFV.select :holdingCoil @01:10:21.31 delay 1 second @01:10:22.34 AS.reconfigure ASfast @01:10:22.39 AS.seek 0ml @01:10:49.75 delay 3 seconds @01:10:52.78 AFV.select :carrierSolution @01:10:52.88 delay 2 seconds @01:10:54.91 AS.reconfigure ASconfig @01:10:54.97 AS.seek 1ml @01:11:52.77 delay 1 second @01:11:53.79 AFV.select :holdingCoil @01:11:53.89 delay 1 second @01:11:54.92 AS.reconfigure ASfast @01:11:54.97 AS.seek 0ml @01:12:22.32 delay 3 seconds @01:12:25.35 AFV.select :carrierSolution @01:12:25.45 delay 2 seconds @01:12:27.48 AS.reconfigure ASconfig @01:12:27.53 AS.seek 1ml @01:13:25.29 delay 1 second @01:13:26.31 AFV.select :holdingCoil @01:13:26.41 delay 1 second @01:13:27.44 AS.reconfigure ASfast @01:13:27.49 AS.seek 0ml @01:13:54.83 delay 3 seconds @01:13:57.86 AFV.select :carrierSolution @01:13:57.96 delay 2 seconds @01:13:59.99 AS.reconfigure ASconfig @01:14:00.05 AS.seek 1ml @01:14:57.83 delay 1 second @01:14:58.85 AFV.select :holdingCoil @01:14:58.95 delay 1 second @01:14:59.98 AS.reconfigure ASfast @01:15:00.03 AS.seek 0ml @01:15:27.37 delay 3 seconds @01:15:30.40 AFV.select :carrierSolution @01:15:30.50 delay 2 seconds @01:15:32.53 AS.reconfigure ASconfig @01:15:32.58 AS.seek 1ml @01:16:30.37 delay 1 second @01:16:31.39 AFV.select :holdingCoil @01:16:31.49 delay 1 second @01:16:32.52 AS.reconfigure ASnorm @01:16:32.57 AS.seek 0ml @01:17:23.28 AFV.select :carrierSolution @01:17:23.39 delay 2 seconds @01:17:25.42 AS.reconfigure ASconfig @01:17:25.47 AS.seek 1ml @01:18:23.30 delay 1 second @01:18:24.32 AFV.select :holdingCoil @01:18:24.42 delay 1 second @01:18:25.45 ATV.dial :waste @01:18:26.62 AS.reconfigure ASnorm @01:18:26.67 AS.seek 0.5ml @01:18:52.23 ATV.dial :waste2 @01:18:54.18 AS.seek 0ml @01:19:19.68 AS.coast @01:19:19.72 SS.coast @01:19:19.76 CC.coast @01:19:19.80 CS.coast @01:19:19.84 PC.coast @01:19:19.89 PS.coast @01:19:19.93 Forearm.coast @01:19:19.97 Elbow.coast @01:19:20.36 Gate.power :core,:OFF @01:19:20.38 Gate.power :raw,:OFF @01:19:20.39 Shutdown @01:19:20.40 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @10:01:17.14
Powering core bus @10:01:21.85 Gate.power :raw,:ON @10:01:22.59 SPE.coast @10:01:22.66 SPE.configure SPEconfig @10:01:22.75 AS.configure ASconfig @10:01:22.85 SS.configure SSconfig @10:01:22.95 CC.configure CCconfig @10:01:23.05 CS.configure CSconfig @10:01:23.16 PC.configure PCconfig @10:01:23.25 PS.configure PSconfig @10:01:23.37 Forearm.configure LooseForearm @10:01:23.47 Elbow.configure LooseElbow @10:01:23.59 SC.configure SCloose @10:01:23.69 SE.configure SEconfig @10:01:23.73 CH.coast @10:01:23.79 CH.configure CHconfig @10:01:23.82 PH.coast @10:01:23.88 PH.configure PHconfig @10:01:23.92 PCR.off @10:01:23.95 PCR.pidtemp= 50,0.1,0,300,25,25 @10:01:23.98 PCR.caltemp= 2.8814,0.0372,0 @10:01:24.01 All dwarf controllers running firmware version 3.75 @10:01:24.12 Valve.reset @10:01:24.29 PCR.off @10:01:24.33 PCR.pidtemp= 50,0.1,0,300,25,25 @10:01:24.36 PCR.caltemp= 2.8814,0.0372,0 @10:01:24.59 Intake.select :close @10:01:24.60 Exhaust.select :close @10:01:24.61 CTV.dial :air @10:01:24.71 PSV.dial :air @10:01:24.74 CSV.dial :waste1 @10:01:24.90
Forearm.reconfigure LooseForearm @10:01:24.96 SSV.dial :spit,avoiding: :exhaust @10:01:24.98
Forearm.home.seek :retract @10:01:25.28 PS.reconfigure PSconfig @10:01:25.33 PS.home.seek 0ml @10:01:25.80 Hand.close @10:01:25.83
Elbow.jog 1500 @10:01:25.98 CS.reconfigure CSfast @10:01:26.02 SS.reconfigure SSconfig @10:01:26.10 CS.home.seek 0ml @10:01:26.14 SS.home.seek 0ml @10:01:26.92
Elbow.reconfigure LooseElbow @10:01:26.97 Elbow.home.seek :clear @10:01:30.45 SE.reconfigure SEconfig @10:01:30.50 SE.home.seek :down @10:01:31.22 SC.reconfigure SCsloppy @10:01:31.27 SC.home.seek :initial @10:01:34.44 PS.coast @10:01:35.50
Forearm.seek :retract @10:01:35.58 Elbow.seek :hammer @10:01:36.90 Forearm.reconfigure TightForearm @10:01:36.95 Forearm.seek 5 + 350 counts @10:01:38.08 Forearm.seek :retract @10:01:39.30 PC.reconfigure PCconfig @10:01:39.31
CC.reconfigure CCconfig @10:01:39.41 PC.home.seek :open @10:01:39.44
CC.home.seek :open @10:01:42.96 Phase [stdphase] @10:01:42.97 GoodNews.email "At 10:01:42 began in tube 3: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:150\n{1 => 22, 2 => 12, 3 => 10}\nCan@10:01:42, 8.4C, 49% humidity, 14.3psia, 13.254V, 0.550A, 0.550A avg, 7.29W",Subject: "Started 20invasivedeployfinal phase stdphase" @10:01:43.01CEST24-May-20
===> STARTED Superphase (phase #14) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @10:01:43.03 Starting initialPurge @10:01:43.04 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:01:43.12 Hand.open @10:01:43.92 Elbow.reconfigure TightElbow @10:01:43.97 Elbow.seek FlushPuck::Garage @10:01:46.05 Elbow.reconfigure LooseElbow @10:01:46.25 Forearm.reconfigure LooseForearm @10:01:46.30 Forearm.seek :garage @10:01:47.30 Hand.close @10:01:48.05 Forearm.seek :retract @10:01:49.08 Elbow.reconfigure TightElbow @10:01:49.17 Elbow.seek CC @10:01:52.25 Elbow.reconfigure LooseElbow @10:01:52.51 Forearm.reconfigure TightForearm @10:01:52.62 Forearm.seek CC @10:01:54.28 CC.seek :closed @10:01:58.74 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.12 @10:01:58.91 Hand.open @10:01:59.71 Forearm.reconfigure LooseForearm @10:01:59.81 Forearm.seek :retract @10:02:00.83 Flushing the Collection Valve::Manifold @10:02:01.15 CSV.dial CSR @10:02:02.74 Pulling 5ml of CSR.flush @10:02:02.76 CSR.select :flush @10:02:02.93 CS.reconfigure CSconfig @10:02:03.07 CS.seek 5ml @10:02:16.86 delay 5 seconds @10:02:21.91 CSR.select :air @10:02:22.19 CS.reconfigure CSfast @10:02:22.32 CS.seek 7.5ml @10:02:29.20 CSV.dial :waste @10:02:30.65 CS.reconfigure CSconfig @10:02:30.75 CS.seek 0ml @10:02:50.93 Purging external Intake @10:02:51.06 CSV.dial :flush @10:02:51.55 CS.reconfigure CSfast @10:02:51.60 CS.seek 10ml @10:03:15.84 delay 5 seconds @10:03:20.87 CSV.dial CBV @10:03:20.91
CBV.dial CSV @10:03:21.74 CTV.dial :intake @10:03:22.55 Intake.select :open @10:03:22.67 CS.seek 0ml @10:03:48.49 Intake.select :close @10:03:48.60 CTV.dial :air @10:03:49.41 Flushing upper manifold @10:03:49.54 CSV.dial :flush @10:03:50.35 CS.seek 5ml @10:04:03.65 delay 5 seconds @10:04:08.68 CSV.dial :air @10:04:09.23 CS.seek 7ml @10:04:14.99 CSV.dial CBV @10:04:15.03
CBV.dial CSV @10:04:16.13 CTV.dial :air @10:04:16.15 CS.seek 0ml @10:04:33.34 delay 7 seconds @10:04:40.38 CS.seek 3ml @10:04:48.37 CSV.dial :waste @10:04:48.86 CS.seek 0ml @10:04:56.96 CSV.dial :flush @10:04:57.43 CS.seek 3ml @10:05:05.37 delay 5 seconds @10:05:10.40 CSV.dial :air @10:05:10.95 CS.seek 5ml @10:05:16.59 CSV.dial :air @10:05:16.61 CS.seek 0ml @10:05:29.21 delay 7 seconds @10:05:36.25 CS.seek 3ml @10:05:44.28 CSV.dial :waste @10:05:45.08 CS.seek 0ml @10:05:53.15 Garaging FlushPuck in Collection Clamp @10:05:53.23 Elbow.reconfigure TightElbow @10:05:53.28 Elbow.seek CC @10:05:53.54 Elbow.reconfigure LooseElbow @10:05:53.65 Forearm.seek CC @10:05:54.62 Hand.close @10:05:55.42 CC.seek :open @10:05:59.78 Forearm.seek :retract @10:06:00.80 Elbow.reconfigure TightElbow @10:06:00.85 Elbow.seek :garage @10:06:04.08 Elbow.reconfigure LooseElbow @10:06:04.18 Forearm.reconfigure TightForearm @10:06:04.23 Forearm.seek :garage @10:06:05.38 Hand.open @10:06:06.24 Forearm.reconfigure LooseForearm @10:06:06.28 Forearm.seek :retract @10:06:07.39 Starting mfb @10:06:07.40 pcrBAC sampling at most 1500ml, wcr at most 1500ml @10:06:07.44 BACfans.email "Started mfb in tube 3 at 10:06:07CEST24-May-20\n{1 => 22, 2 => 12, 3 => 10}\nCan@10:05:46, 8.4C, 49% humidity, 14.4psia, 13.205V, 0.651A, 0.657A avg, 8.60W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @10:06:07.51
Loading sh1 puck from tube 3 into Collection Clamp @10:06:07.64 Readying the MFB @10:06:07.68 Homing MFB Syringe @10:06:07.73 AFV.select :holdingCoil @10:06:07.96 ATV.dial :air2 @10:06:08.01
SC.reconfigure SCtight @10:06:08.12 SC.seek 3 @10:06:08.60 AS.reconfigure ASconfig @10:06:08.65 AS.home.seek :home @10:06:10.28
SC.reconfigure SCloose @10:06:10.53 Elbow.reconfigure TightElbow @10:06:10.60 Elbow.seek 3 @10:06:13.74 Forearm.reconfigure TightForearm @10:06:13.79 Forearm.seek 3 + 700 counts @10:06:14.23 AFV.select :carrierSolution @10:06:14.40 delay 2 seconds @10:06:14.94
SE.reconfigure SEpick @10:06:15.14 SE.seek beamBreak @10:06:16.46 AS.seek 1ml @10:06:17.64
SE.configure SEconfig @10:06:18.16 Elbow.seek 3 @10:06:18.64 Forearm.seek 3 + 300 counts @10:06:18.93 Forearm.reconfigure LooseForearm @10:06:19.05 Hand.close @10:06:19.80 SE.seek :down @10:06:20.30
Forearm.seek :retract @10:06:21.39 Elbow.seek CC @10:06:21.94 SE.coast @10:06:23.25
Elbow.reconfigure LooseElbow @10:06:23.51 Forearm.reconfigure TightForearm @10:06:23.61 Forearm.seek CC @10:06:25.36 CC.seek :closed @10:06:29.88 Collection Clamp onSpring/offSpring for 6/30 samples, on/off torque 2.24 @10:06:29.92 Hand.open @10:06:30.83 Forearm.reconfigure LooseForearm @10:06:30.97 Forearm.seek :retract @10:06:32.02 SC.coast @10:06:32.10 collectSample 1500ml w/bubble point of 55psi @10:06:32.13 Priming 7ml @10:06:32.15 Intake.select :close @10:06:32.17 Exhaust.select :close @10:06:32.20 SS.hold @10:06:32.72 Calibrating pressure and temperature sensors @10:06:32.76 CTV.dial :air @10:06:32.78 SSV.dial :spit,avoiding: :exhaust @10:06:32.82 SS.reconfigure SSfast @10:06:32.93 SS.seek 5ml @10:06:35.47 SSV.dial CBV @10:06:35.54
CBV.dial SSV @10:06:37.09 delay 3.8 seconds @10:06:41.16 SSV.dial :spit @10:06:42.66 SS.seek 0ml @10:06:45.42 SSV.dial CBV @10:06:46.89 CTV.dial :intake @10:06:47.78 SSV.dial CBV @10:06:47.85
CBV.dial SSV @10:06:47.88 Allowing 40s for 7.0ml of remaining 7.0ml @10:06:47.90 Intake.select :open @10:06:50.22 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:06:50.26 SS.reconfigure SSinhale.with maxSpeed: 150 @10:06:50.35 SS.seek 7ml @10:06:53.96 Vacuum too high -- waiting up to 40s @10:06:56.82 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:06:56.85 SS.reconfigure SSinhale.with maxSpeed: 135 @10:06:56.96 SS.seek 7ml @10:07:00.15 Vacuum too high -- waiting up to 40s @10:07:03.10 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:07:03.17 SS.reconfigure SSinhale.with maxSpeed: 122 @10:07:03.27 SS.seek 7ml @10:07:03.91 Stroke completed -- waiting for pressure to equalize ... @10:07:07.15 Intake.select :close @10:07:07.40 SSV.dial :exhaust @10:07:08.27 Exhaust.select :open @10:07:08.44 SS.reconfigure SSexhale @10:07:08.53 SS.seek 0ml @10:07:09.41 delay 1 second @10:07:10.44 AFV.select :holdingCoil @10:07:10.57 delay 1 second @10:07:11.62 ATV.dial :waste @10:07:12.33
Exhaust.select :close @10:07:12.50 Sampling 1500.0ml @10:07:12.54 Intake.select :close @10:07:12.57 Exhaust.select :close @10:07:12.72 SS.reconfigure SSconfig @10:07:12.83 AS.reconfigure ASnorm @10:07:12.94
SS.hold @10:07:13.13 AS.home.seek :empty @10:07:13.56
CTV.dial :intake @10:07:13.61 SSV.dial CBV @10:07:13.66
CBV.dial SSV @10:07:14.58 Allowing 130s for 25.0ml of remaining 1500.0ml @10:07:14.60 Intake.select :open @10:07:16.88 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:07:16.91 SS.reconfigure SSinhale.with maxSpeed: 122 @10:07:17.01 SS.seek 25ml @10:07:20.59 Vacuum too high -- waiting up to 40s @10:07:22.90 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:07:22.91 SS.reconfigure SSinhale.with maxSpeed: 110 @10:07:22.96 SS.seek 25ml @10:07:25.52 Vacuum too high -- waiting up to 40s @10:07:27.88 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:07:27.89 SS.reconfigure SSinhale.with maxSpeed: 99 @10:07:27.94 SS.seek 25ml @10:07:30.74 Vacuum too high -- waiting up to 40s @10:07:33.00 SS.reconfigure SSinhale.with maxSpeed: 89 @10:07:33.05 SS.seek 25ml @10:07:36.17 Vacuum too high -- waiting up to 40s @10:07:38.30 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:07:38.32 SS.reconfigure SSinhale.with maxSpeed: 80 @10:07:38.37 SS.seek 25ml @10:07:41.17 Vacuum too high -- waiting up to 40s @10:07:43.33 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:07:43.35 SS.reconfigure SSinhale.with maxSpeed: 72 @10:07:43.40 SS.seek 25ml @10:07:46.39 Vacuum too high -- waiting up to 40s @10:07:48.50 SS.reconfigure SSinhale.with maxSpeed: 65 @10:07:48.55 SS.seek 25ml @10:07:52.17 Vacuum too high -- waiting up to 40s @10:07:54.26 SS.reconfigure SSinhale.with maxSpeed: 59 @10:07:54.31 SS.seek 25ml @10:07:57.57 Vacuum too high -- waiting up to 40s @10:07:59.65 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:07:59.67 SS.reconfigure SSinhale.with maxSpeed: 53 @10:07:59.72 SS.seek 25ml @10:08:04.02 Vacuum too high -- waiting up to 40s @10:08:05.73 ATV.dial :waste @10:08:05.75 ARV.dial between carrier and air1 @10:08:05.84 ASV.dial :waste1 @10:08:06.11
Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:08:06.13 SS.reconfigure SSinhale.with maxSpeed: 48 @10:08:06.18 SS.seek 25ml @10:08:08.30 Stroke completed -- waiting for pressure to equalize ... @10:08:11.52 Intake.select :close @10:08:11.67 SSV.dial :exhaust @10:08:12.50 Exhaust.select :open @10:08:12.62 SS.reconfigure SSexhale @10:08:12.66 SS.seek 0ml @10:08:21.72 Exhaust.select :close @10:08:21.84 SSV.dial CBV @10:08:21.86
CBV.dial SSV @10:08:22.72 Allowing 130s for 25.0ml of remaining 1475.0ml @10:08:22.73 Intake.select :open @10:08:24.90 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:08:24.92 SS.reconfigure SSinhale.with maxSpeed: 48 @10:08:24.97 SS.seek 25ml @10:08:31.30 Vacuum too high -- waiting up to 40s @10:08:33.11 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:08:33.13 SS.reconfigure SSinhale.with maxSpeed: 43 @10:08:33.18 SS.seek 25ml @10:08:38.33 Vacuum too high -- waiting up to 40s @10:08:40.42 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:08:40.44 SS.reconfigure SSinhale.with maxSpeed: 39 @10:08:40.49 SS.seek 25ml @10:08:46.55 Vacuum too high -- waiting up to 40s @10:08:48.61 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:08:48.63 SS.reconfigure SSinhale.with maxSpeed: 35 @10:08:48.67 SS.seek 25ml @10:08:55.24 Vacuum too high -- waiting up to 40s @10:08:57.47 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:08:57.49 SS.reconfigure SSinhale.with maxSpeed: 32 @10:08:57.54 SS.seek 25ml @10:09:04.89 Vacuum too high -- waiting up to 40s @10:09:07.25 SS.reconfigure SSinhale.with maxSpeed: 29 @10:09:07.30 SS.seek 25ml @10:09:15.99 Vacuum too high -- waiting up to 40s @10:09:18.55 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:09:18.57 SS.reconfigure SSinhale.with maxSpeed: 26 @10:09:18.62 SS.seek 25ml @10:09:30.83 Vacuum too high -- waiting up to 40s @10:09:33.40 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:09:33.42 SS.reconfigure SSinhale.with maxSpeed: 23 @10:09:33.46 SS.seek 25ml @10:09:36.10 Stroke completed -- waiting for pressure to equalize ... @10:09:41.82 Intake.select :close @10:09:41.98 SSV.dial :exhaust @10:09:42.81 Exhaust.select :open @10:09:42.93 SS.reconfigure SSexhale @10:09:42.98 SS.seek 0ml @10:09:52.34 Exhaust.select :close @10:09:52.46 SSV.dial CBV @10:09:52.48
CBV.dial SSV @10:09:53.31 Allowing 130s for 25.0ml of remaining 1450.0ml @10:09:53.32 Intake.select :open @10:09:55.49 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:09:55.51 SS.reconfigure SSinhale.with maxSpeed: 23 @10:09:55.55 SS.seek 25ml @10:10:21.27 Vacuum too high -- waiting up to 40s @10:10:23.52 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:10:23.53 SS.reconfigure SSinhale.with maxSpeed: 21 @10:10:23.58 SS.seek 25ml @10:11:07.93 Vacuum too high -- waiting up to 40s @10:11:10.51 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:11:10.53 SS.reconfigure SSinhale.with maxSpeed: 19 @10:11:10.58 SS.seek 25ml @10:11:24.09 Stroke completed -- waiting for pressure to equalize ... @10:11:31.63 Intake.select :close @10:11:31.79 SSV.dial :exhaust @10:11:32.61 Exhaust.select :open @10:11:32.73 SS.reconfigure SSexhale @10:11:32.77 SS.seek 0ml @10:11:42.33 Exhaust.select :close @10:11:42.45 SSV.dial CBV @10:11:42.47
CBV.dial SSV @10:11:43.33 Allowing 130s for 25.0ml of remaining 1425.0ml @10:11:43.34 Intake.select :open @10:11:45.51 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:11:45.53 SS.reconfigure SSinhale.with maxSpeed: 19 @10:11:45.57 SS.seek 25ml @10:13:17.55 Stroke completed -- waiting for pressure to equalize ... @10:13:25.10 Intake.select :close @10:13:25.26 SSV.dial :exhaust @10:13:26.09 Exhaust.select :open @10:13:26.21 SS.reconfigure SSexhale @10:13:26.25 SS.seek 0ml @10:13:35.45 Exhaust.select :close @10:13:36.23 SSV.dial CBV @10:13:36.25
CBV.dial SSV @10:13:37.11 Allowing 130s for 25.0ml of remaining 1400.0ml @10:13:37.12 Intake.select :open @10:13:39.30 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:13:39.32 SS.reconfigure SSinhale.with maxSpeed: 19 @10:13:39.36 SS.seek 25ml @10:15:11.19 Stroke completed -- waiting for pressure to equalize ... @10:15:18.22 Intake.select :close @10:15:18.38 SSV.dial :exhaust @10:15:19.22 Exhaust.select :open @10:15:19.34 SS.reconfigure SSexhale @10:15:19.38 SS.seek 0ml @10:15:28.64 Exhaust.select :close @10:15:28.76 SSV.dial CBV @10:15:28.81
CBV.dial SSV @10:15:29.59 Allowing 130s for 25.0ml of remaining 1375.0ml @10:15:29.60 Intake.select :open @10:15:31.78 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:15:31.79 SS.reconfigure SSinhale.with maxSpeed: 19 @10:15:31.84 SS.seek 25ml @10:17:03.69 Stroke completed -- waiting for pressure to equalize ... @10:17:10.01 Intake.select :close @10:17:10.17 SSV.dial :exhaust @10:17:11.00 Exhaust.select :open @10:17:11.12 SS.reconfigure SSexhale @10:17:11.16 SS.seek 0ml @10:17:20.86 Exhaust.select :close @10:17:20.98 SSV.dial CBV @10:17:21.01
CBV.dial SSV @10:17:21.84 Allowing 130s for 25.0ml of remaining 1350.0ml @10:17:21.85 Intake.select :open @10:17:24.02 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:17:24.04 SS.reconfigure SSinhale.with maxSpeed: 19 @10:17:24.09 SS.seek 25ml @10:18:56.05 Stroke completed -- waiting for pressure to equalize ... @10:19:02.35 Intake.select :close @10:19:02.50 SSV.dial :exhaust @10:19:03.33 Exhaust.select :open @10:19:03.45 SS.reconfigure SSexhale @10:19:03.49 SS.seek 0ml @10:19:12.44 Exhaust.select :close @10:19:13.21 SSV.dial CBV @10:19:13.26
CBV.dial SSV @10:19:14.06 Allowing 130s for 25.0ml of remaining 1325.0ml @10:19:14.07 Intake.select :open @10:19:16.24 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:19:16.26 SS.reconfigure SSinhale.with maxSpeed: 19 @10:19:16.31 SS.seek 25ml @10:20:48.57 Stroke completed -- waiting for pressure to equalize ... @10:20:54.94 Intake.select :close @10:20:55.10 SSV.dial :exhaust @10:20:55.94 Exhaust.select :open @10:20:56.06 SS.reconfigure SSexhale @10:20:56.10 SS.seek 0ml @10:21:05.33 Exhaust.select :close @10:21:05.45 SSV.dial CBV @10:21:05.47
CBV.dial SSV @10:21:06.30 Allowing 130s for 25.0ml of remaining 1300.0ml @10:21:06.31 Intake.select :open @10:21:08.48 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:21:08.50 SS.reconfigure SSinhale.with maxSpeed: 19 @10:21:08.55 SS.seek 25ml @10:22:40.40 Stroke completed -- waiting for pressure to equalize ... @10:22:47.37 Intake.select :close @10:22:47.53 SSV.dial :exhaust @10:22:48.36 Exhaust.select :open @10:22:48.48 SS.reconfigure SSexhale @10:22:48.52 SS.seek 0ml @10:22:57.81 Exhaust.select :close @10:22:57.93 SSV.dial CBV @10:22:57.95
CBV.dial SSV @10:22:58.80 Allowing 130s for 25.0ml of remaining 1275.0ml @10:22:58.81 Intake.select :open @10:23:00.98 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:23:01.00 SS.reconfigure SSinhale.with maxSpeed: 19 @10:23:01.04 SS.seek 25ml @10:23:32.77 Session Begun @10:23:37.80 -> showlog 100 @10:24:33.10
Stroke completed -- waiting for pressure to equalize ... @10:24:40.64 Intake.select :close @10:24:41.53 SSV.dial :exhaust @10:24:42.39 Exhaust.select :open @10:24:42.54 SS.reconfigure SSexhale @10:24:42.63 SS.seek 0ml @10:24:47.24 -> exit @10:24:47.26 Session Ended @10:24:51.91
Exhaust.select :close @10:24:52.02 SSV.dial CBV @10:24:52.06
CBV.dial SSV @10:24:52.89 Allowing 130s for 25.0ml of remaining 1250.0ml @10:24:52.90 Intake.select :open @10:24:55.07 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:24:55.09 SS.reconfigure SSinhale.with maxSpeed: 19 @10:24:55.14 SS.seek 25ml @10:26:27.26 Stroke completed -- waiting for pressure to equalize ... @10:26:36.10 Intake.select :close @10:26:36.26 SSV.dial :exhaust @10:26:37.10 Exhaust.select :open @10:26:37.22 SS.reconfigure SSexhale @10:26:37.26 SS.seek 0ml @10:26:46.49 Exhaust.select :close @10:26:46.61 SSV.dial CBV @10:26:46.66
CBV.dial SSV @10:26:47.46 Allowing 130s for 25.0ml of remaining 1225.0ml @10:26:47.47 Intake.select :open @10:26:49.64 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:26:49.66 SS.reconfigure SSinhale.with maxSpeed: 19 @10:26:49.71 SS.seek 25ml @10:28:21.68 Stroke completed -- waiting for pressure to equalize ... @10:28:31.71 Intake.select :close @10:28:31.86 SSV.dial :exhaust @10:28:32.69 Exhaust.select :open @10:28:32.81 SS.reconfigure SSexhale @10:28:32.85 SS.seek 0ml @10:28:41.88 Exhaust.select :close @10:28:42.00 SSV.dial CBV @10:28:42.03
CBV.dial SSV @10:28:42.86 Allowing 130s for 25.0ml of remaining 1200.0ml @10:28:42.87 Intake.select :open @10:28:45.04 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:28:45.06 SS.reconfigure SSinhale.with maxSpeed: 19 @10:28:45.11 SS.seek 25ml @10:29:46.18 Vacuum too high -- waiting up to 40s @10:29:48.70 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:29:48.72 SS.reconfigure SSinhale.with maxSpeed: 17 @10:29:48.76 SS.seek 25ml @10:30:24.18 Stroke completed -- waiting for pressure to equalize ... @10:30:33.65 Intake.select :close @10:30:33.81 SSV.dial :exhaust @10:30:34.65 Exhaust.select :open @10:30:34.77 SS.reconfigure SSexhale @10:30:34.81 SS.seek 0ml @10:30:43.88 Exhaust.select :close @10:30:44.00 SSV.dial CBV @10:30:44.02
CBV.dial SSV @10:30:44.86 Allowing 130s for 25.0ml of remaining 1175.0ml @10:30:44.87 Intake.select :open @10:30:47.04 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:30:47.06 SS.reconfigure SSinhale.with maxSpeed: 17 @10:30:47.10 SS.seek 25ml @10:32:30.75 Stroke completed -- waiting for pressure to equalize ... @10:32:42.05 Intake.select :close @10:32:42.21 SSV.dial :exhaust @10:32:43.05 Exhaust.select :open @10:32:43.17 SS.reconfigure SSexhale @10:32:43.21 SS.seek 0ml @10:32:52.47 Exhaust.select :close @10:32:52.59 SSV.dial CBV @10:32:52.61
CBV.dial SSV @10:32:53.46 Allowing 130s for 25.0ml of remaining 1150.0ml @10:32:53.47 Intake.select :open @10:32:56.21 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:32:56.33 SS.reconfigure SSinhale.with maxSpeed: 17 @10:32:56.38 SS.seek 25ml @10:33:56.68 Vacuum too high -- waiting up to 40s @10:33:59.53 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:33:59.55 SS.reconfigure SSinhale.with maxSpeed: 15 @10:33:59.60 SS.seek 25ml @10:34:48.56 Stroke completed -- waiting for pressure to equalize ... @10:35:00.51 Intake.select :close @10:35:00.67 SSV.dial :exhaust @10:35:01.51 Exhaust.select :open @10:35:01.63 SS.reconfigure SSexhale @10:35:01.67 SS.seek 0ml @10:35:11.12 Exhaust.select :close @10:35:11.24 SSV.dial CBV @10:35:11.27
CBV.dial SSV @10:35:12.12 Allowing 130s for 25.0ml of remaining 1125.0ml @10:35:12.13 Intake.select :open @10:35:14.31 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:35:14.32 SS.reconfigure SSinhale.with maxSpeed: 15 @10:35:14.38 SS.seek 25ml @10:36:58.34 Vacuum too high -- waiting up to 40s @10:37:01.15 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:37:01.17 SS.reconfigure SSinhale.with maxSpeed: 14 @10:37:01.22 SS.seek 25ml @10:37:15.92 Stroke completed -- waiting for pressure to equalize ... @10:37:22.14 SS.reconfigure SSconfig @10:37:22.20 SS.hold @10:37:22.33 Intake.select :close @10:37:22.49 ShallowSampler::Clogged in MAIN -- progress too slow after 400.0ml @10:37:22.50 SSV.dial :exhaust @10:37:23.35 Exhaust.select :open @10:37:23.47 SS.reconfigure SSexhale @10:37:23.51 SS.seek 0ml @10:37:33.24 Exhaust.select :close @10:37:33.35 Sampled 400.0ml @10:37:33.37 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:37:33.38 Intake.select :close @10:37:33.45 CSV.dial :waste @10:37:33.51 CS.seek 0ml @10:37:33.61 SSV.dial CBV,avoiding: :exhaust @10:37:33.64
CBV.dial SSV @10:37:33.88 CSV.dial :air @10:37:34.68 CS.seek 10ml @10:37:35.68
CTV.dial :air @10:37:36.50 delay 15 seconds @10:37:51.53 SSV.dial :spit,avoiding: :exhaust @10:37:52.95 CTV.dial CSV,avoiding: :intake @10:37:53.03
SS.seek 0ml @10:37:53.11 SSV.dial CBV,avoiding: :exhaust @10:37:59.73 CBV.dial between SSV and waste2 @10:38:00.15 CTV.dial CSV,avoiding: :air @10:38:00.17
CSV.dial CTV @10:38:00.67 CS.reconfigure CSpressurize @10:38:00.71 CS.seek 0ml @10:38:26.44 BubblePoint: Intake @69.8psia, Outlet @14.3psia @10:38:26.45 CBV.dial SSV @10:38:26.82 SS.reconfigure SSconfig @10:38:26.88 SS.seek 10ml @10:38:32.33 delay 15 seconds @10:38:47.47 Evacuated: Intake @36.1psia, Outlet @0.7psia @10:38:47.48 SSV.dial :spit,avoiding: :exhaust @10:38:48.91 SS.reconfigure SSexhale @10:38:48.96 SS.seek 0ml @10:38:53.97 SS.reconfigure SSfast @10:38:54.02 SS.seek 5ml @10:38:56.91 SSV.dial CBV,avoiding: :exhaust @10:38:56.94
CBV.dial SSV @10:38:58.37 CTV.dial :air,avoiding: :intake @10:38:59.80 CSV.dial CTV @10:38:59.85
SS.seek 10ml @10:39:00.85 CSV.dial :air @10:39:01.35 CS.reconfigure CSfast @10:39:01.40 CS.seek 0ml @10:39:02.69
SSV.dial :spit,avoiding: :exhaust @10:39:02.76 CS.coast @10:39:04.12
SS.reconfigure SSexhale @10:39:04.17 SS.seek 0ml @10:39:09.49 SS.reconfigure SSfast @10:39:09.54 SS.seek 5ml @10:39:12.05 SSV.dial CBV,avoiding: :exhaust @10:39:12.08
CBV.dial SSV @10:39:15.09 SSV.dial :spit,avoiding: :exhaust @10:39:16.68 SS.reconfigure SSconfig @10:39:16.73 SS.seek 0ml @10:39:19.88 SS.coast @10:39:19.92 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:39:19.93 Intake.select :close @10:39:20.02 CSV.dial :waste @10:39:20.15 SSV.dial CBV,avoiding: :exhaust @10:39:20.20
CBV.dial SSV @10:39:20.87 CS.seek 0ml @10:39:20.94 CSV.dial :air @10:39:21.60
CTV.dial :air @10:39:21.62 delay 15 seconds @10:39:21.73 CS.seek 10ml @10:39:36.64
SSV.dial :spit,avoiding: :exhaust @10:39:38.06 CTV.dial CSV,avoiding: :intake @10:39:38.10
SS.reconfigure SSexhale @10:39:38.20 SS.seek 0ml @10:39:38.27 SSV.dial CBV,avoiding: :exhaust @10:39:46.44 CBV.dial between SSV and waste2 @10:39:46.87 CSV.dial between air and CTV @10:39:47.22 CS.seek 8.249ml @10:39:52.48 CTV.dial CSV,avoiding: :air @10:39:52.50
CSV.dial CTV @10:39:52.84 CS.reconfigure CSpressurize @10:39:52.88 CS.seek 0ml @10:40:14.63 CSV.dial :air @10:40:15.12 CS.reconfigure CSfast @10:40:15.17 CS.seek 10ml @10:40:39.58 CSV.dial between air and CTV @10:40:39.92 CS.seek 3.042ml @10:40:57.20 CTV.dial CSV,avoiding: :air @10:40:57.22
CSV.dial CTV @10:40:57.69 CS.reconfigure CSpressurize @10:40:57.73 CS.seek 0ml @10:41:05.03 BubblePoint: Intake @69.5psia, Outlet @13.9psia @10:41:05.04 CBV.dial SSV @10:41:05.38 SS.reconfigure SSconfig @10:41:05.43 SS.seek 10ml @10:41:11.05 delay 15 seconds @10:41:26.20 Evacuated: Intake @41.3psia, Outlet @14.3psia @10:41:26.21 SSV.dial :spit,avoiding: :exhaust @10:41:27.63 SS.reconfigure SSexhale @10:41:27.68 SS.seek 0ml @10:41:33.00 SS.reconfigure SSfast @10:41:33.05 SS.seek 5ml @10:41:35.73 SSV.dial CBV,avoiding: :exhaust @10:41:35.76
CBV.dial SSV @10:41:37.20 CTV.dial :air,avoiding: :intake @10:41:38.65 CSV.dial CTV @10:41:38.70
SS.seek 10ml @10:41:39.70 CSV.dial :air @10:41:40.18 CS.reconfigure CSfast @10:41:40.23 CS.seek 0ml @10:41:41.29
SSV.dial :spit,avoiding: :exhaust @10:41:42.31 CS.coast @10:41:42.71
SS.reconfigure SSexhale @10:41:42.77 SS.seek 0ml @10:41:48.55 SS.reconfigure SSfast @10:41:48.60 SS.seek 5ml @10:41:51.20 SSV.dial CBV,avoiding: :exhaust @10:41:51.23
CBV.dial SSV @10:41:53.66 SSV.dial :spit,avoiding: :exhaust @10:41:55.07 SS.reconfigure SSconfig @10:41:55.13 SS.seek 0ml @10:41:58.29 SS.coast @10:41:58.33 Lysing: Adding 1.5ml lysis @10:41:58.34 CBV.dial between CSV and waste1,avoiding: :DBV @10:41:59.95 CTV.dial CSV @10:42:01.38 CSV.dial CSR @10:42:02.22 Pulling 1ml of CSR.lysis @10:42:02.23 CSR.select :lysis @10:42:02.36 CS.reconfigure CSconfig @10:42:02.40 CS.seek 1ml @10:42:05.95 delay 5 seconds @10:42:10.98 CSR.select :air @10:42:11.16 CS.seek 6ml @10:42:24.85 delay 2 seconds @10:42:26.87 CSV.dial :waste1 @10:42:28.26 CS.reconfigure CSfast @10:42:28.31 CS.seek :empty @10:42:43.29 CSV.dial CSR @10:42:44.73 Pulling 1.5ml of CSR.lysis @10:42:44.74 CSR.select :lysis @10:42:44.86 CS.reconfigure CSconfig @10:42:44.90 CS.seek 1.5ml @10:42:49.73 delay 5 seconds @10:42:54.76 CSR.select :air @10:42:54.93 CS.seek 4.5ml @10:43:03.67 delay 2 seconds @10:43:05.69 CSV.dial CTV @10:43:06.19 CS.seek 1.275ml @10:43:15.77 Heating sh1 puck from tube 3 in Collection Clamp to 85C for 5:00 @10:43:15.79 CH.seek 85C,8:00 @10:46:39.97 CH.hold 5:00,85C @10:51:40.14 Collection Puck Heater at 85.1C after lysing @10:51:40.16 CS.seek 0ml @10:51:44.46 CTV.dial between spare and air,avoiding: :intake @10:51:45.58 CSV.dial :air @10:51:46.09 CS.seek 5ml @10:51:59.80 CSV.dial CTV @10:52:00.29 delay 2 seconds @10:52:02.31 CSV.dial :air @10:52:02.80 delay 2 seconds @10:52:04.82 CSV.dial CTV @10:52:05.83 delay 2 seconds @10:52:07.85 CSV.dial :air @10:52:08.35 delay 2 seconds @10:52:10.38 CS.seek 0ml @10:52:24.15 Recovering Lysate into Collection syringe @10:52:24.16 CSV.dial CBV @10:52:25.26 CBV.dial CSV @10:52:25.69 CS.seek 5ml @10:52:39.52 delay 5 seconds @10:52:44.54 CTV.dial :air @10:52:45.11 CS.seek 7ml @10:52:51.18 delay 5 seconds @10:52:56.21 CSV.dial :air2 @10:52:56.70 CS.seek 10ml @10:53:05.30 CSV.dial between air and CTV,avoiding: CBV @10:53:06.54 Lysing Complete @10:53:06.62 Storing sh1 puck in Collection Clamp to tube 2 @10:53:06.81 SC.reconfigure SCtight @10:53:06.86 SC.seek 2 @10:53:09.05 SC.reconfigure SCloose @10:53:09.27 Elbow.reconfigure TightElbow @10:53:09.32 Elbow.seek 2 @10:53:11.24 Forearm.reconfigure TightForearm @10:53:11.29 Forearm.seek 2 + 700 counts @10:53:12.36 SE.reconfigure SEpick @10:53:12.44 SE.seek beamBreak @10:53:14.46 SE.configure SEconfig @10:53:14.62 Elbow.reconfigure LimpElbow @10:53:14.67 Forearm.reconfigure LooseForearm @10:53:14.72 Forearm.seek 2 @10:53:14.99 Elbow.hold @10:53:15.06 Elbow.reconfigure TightElbow @10:53:15.82 SE.seek 11.08 Pucks below Top Plate @10:53:16.27 Forearm.force -20 @10:53:16.31 Forearm.seek 2 + 345 counts @10:53:16.63 Forearm.reconfigure TightForearm @10:53:16.68 Forearm.seek 2 + 700 counts @10:53:16.98 SE.seek 12.08 Pucks below Top Plate @10:53:17.62 Forearm.reconfigure LooseForearm @10:53:17.67 Forearm.seek :retract @10:53:18.61 Elbow.seek CC @10:53:20.32 Elbow.reconfigure LooseElbow @10:53:20.43 Forearm.seek CC @10:53:21.49 Hand.close @10:53:22.26 CC.seek :open @10:53:26.78 Forearm.seek :retract @10:53:27.82 Elbow.reconfigure TightElbow @10:53:27.87 Elbow.seek 2 @10:53:29.78 Elbow.reconfigure LimpElbow @10:53:29.83 Forearm.seek 2 @10:53:30.85 Elbow.hold @10:53:30.93 Elbow.reconfigure TightElbow @10:53:30.97 Hand.open @10:53:31.90 Forearm.force -20 @10:53:31.94 Forearm.seek 2 + 339 counts @10:53:32.27 Forearm.reconfigure TightForearm @10:53:32.31 Forearm.seek 2 + 700 counts @10:53:32.69 SE.seek :down @10:53:33.83
Forearm.reconfigure LooseForearm @10:53:33.88 Forearm.seek :retract @10:53:34.37 SE.coast @10:53:34.79
SC.coast @10:53:34.83 Tranferring lysate to PRV @10:53:34.84 CSV.dial :air @10:53:35.30 CS.seek 10ml @10:53:35.37 CSV.dial between air and CTV @10:53:35.82 AIV.dial :bypass @10:53:35.91 Beginning samplePassC @10:53:35.92 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:53:36.00 Hand.open @10:53:36.40 Elbow.seek FlushPuck::Garage @10:53:38.41 Elbow.reconfigure LooseElbow @10:53:38.52 Forearm.seek :garage @10:53:39.49 Hand.close @10:53:40.84 Forearm.seek :retract @10:53:41.91 Elbow.reconfigure TightElbow @10:53:41.96 Elbow.seek CC @10:53:44.83 Elbow.reconfigure LooseElbow @10:53:44.94 Forearm.reconfigure TightForearm @10:53:45.00 Forearm.seek CC @10:53:46.32 CC.seek :closed @10:53:50.58 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.08 @10:53:50.66 Hand.open @10:53:51.45 Forearm.reconfigure LooseForearm @10:53:51.50 Forearm.seek :retract @10:53:52.52 AWV.select :air @10:53:52.53 AIV.dial :bypass @10:53:52.54 PSV.dial PSR @10:53:54.11 Pulling 1.5ml of PSR.flush @10:53:54.12 PSR.select :flush @10:53:54.25 PS.seek 1.5ml @10:54:10.31 delay 5 seconds @10:54:15.34 PSV.dial :waste1 @10:54:15.84 PS.seek 0ml @10:54:31.81 PSV.dial PSR @10:54:32.30 PSR.select :air @10:54:32.42 PS.seek 2.5ml @10:54:58.57 PRV.dial CBV @10:54:58.59
CBV.dial PRV @10:54:59.48 PSV.dial :mixloop @10:55:00.56 CTV.dial :air,avoiding: :intake @10:55:00.57 PTV.dial :PRVmixing @10:55:00.67 PS.seek 0ml @10:55:26.85 PSV.dial :air @10:55:27.34 PS.seek 2.5ml @10:55:53.44 PSV.dial :mixloop @10:55:53.92 PS.seek 0ml @10:56:20.00 PSV.dial :air @10:56:20.48 PS.seek 2.5ml @10:56:46.60 PSV.dial :mixloop @10:56:47.09 PS.seek 0ml @10:57:13.25 Garaging FlushPuck in Collection Clamp @10:57:13.33 Elbow.reconfigure TightElbow @10:57:13.38 Elbow.seek CC @10:57:13.60 Elbow.reconfigure LooseElbow @10:57:13.71 Forearm.seek CC @10:57:14.73 Hand.close @10:57:15.51 CC.seek :open @10:57:19.88 Forearm.seek :retract @10:57:20.90 Elbow.reconfigure TightElbow @10:57:20.95 Elbow.seek :garage @10:57:24.10 Elbow.reconfigure LooseElbow @10:57:24.20 Forearm.reconfigure TightForearm @10:57:24.25 Forearm.seek :garage @10:57:25.42 Hand.open @10:57:26.87 Forearm.reconfigure LooseForearm @10:57:26.92 Forearm.seek :retract @10:57:28.01 Connecting PS to CS to transfer sample @10:57:28.02 CBV.dial AIV @10:57:28.03 AIV.dial :bypass @10:57:28.05 PSV.dial :mixloop @10:57:28.06 PTV.dial :PRVmixing @10:57:28.07 PRV.dial AIV @10:57:28.09 CTV.dial :air,avoiding: :intake @10:57:28.11 Loading lyfil puck from tube 3 into Collection Clamp @10:57:28.36 SC.reconfigure SCtight @10:57:28.41 SC.seek 3 @10:57:30.59 SC.reconfigure SCloose @10:57:30.81 Elbow.reconfigure TightElbow @10:57:30.86 Elbow.seek 3 @10:57:33.03 Forearm.reconfigure TightForearm @10:57:33.08 Forearm.seek 3 + 700 counts @10:57:34.11 SE.reconfigure SEpick @10:57:34.19 SE.seek beamBreak @10:57:36.56 SE.configure SEconfig @10:57:36.72 Elbow.seek 3 @10:57:36.93 Forearm.seek 3 + 300 counts @10:57:37.23 Forearm.reconfigure LooseForearm @10:57:37.27 Hand.close @10:57:37.91 SE.seek :down @10:57:38.14
Forearm.seek :retract @10:57:39.09 Elbow.seek CC @10:57:40.00 SE.coast @10:57:40.72
Elbow.reconfigure LooseElbow @10:57:40.82 Forearm.reconfigure TightForearm @10:57:40.87 Forearm.seek CC @10:57:42.22 CC.seek :closed @10:57:46.48 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.16 @10:57:46.49 Hand.open @10:57:47.34 Forearm.reconfigure LooseForearm @10:57:47.39 Forearm.seek :retract @10:57:48.40 SC.coast @10:57:48.43 CTV.dial CSV,avoiding: :intake @10:57:49.87 CSV.dial CTV @10:57:50.21 CS.seek 5ml @10:58:03.93 delay 1:20 @10:59:23.97 CS.seek 2.5ml @10:59:31.34 delay 40 seconds @11:00:11.38 CS.seek 0ml @11:00:18.94 delay 1:00 @11:01:18.97 PSV.dial :air @11:01:19.46 PS.seek 2.5ml @11:01:45.57 PSV.dial :mixloop @11:01:46.04 delay 3 seconds @11:01:49.06 PSV.dial :air @11:01:49.54 delay 2 seconds @11:01:51.56 PSV.dial :mixloop @11:01:52.04 delay 3 seconds @11:01:55.06 PSV.dial :waste1 @11:01:56.45 PS.seek :empty @11:02:23.21 CBV.dial :waste1 @11:02:23.81 delay 5 seconds @11:02:28.84 CBV.dial AIV @11:02:29.35 delay 10 seconds @11:02:39.38 CBV.dial :waste1 @11:02:39.89 delay 10 seconds @11:02:49.92 CBV.dial AIV @11:02:50.42 delay 10 seconds @11:03:00.45 CTV.dial CSV,avoiding: :intake @11:03:00.46 CBV.dial between AIV and waste1 @11:03:00.83 CSV.dial :air @11:03:01.34 CS.seek 10ml @11:03:27.94 CSV.dial CTV @11:03:28.43 CS.seek 0ml @11:03:54.94 CBV.dial :waste1 @11:03:55.27 delay 10 seconds @11:04:05.30 CTV.dial :air,avoiding: :intake @11:04:06.81 Storing lyfil puck in Collection Clamp to tube 2 @11:04:07.00 SC.reconfigure SCtight @11:04:07.05 SC.seek 2 @11:04:09.26 SC.reconfigure SCloose @11:04:09.48 Elbow.reconfigure TightElbow @11:04:09.53 Elbow.seek 2 @11:04:11.50 Forearm.reconfigure TightForearm @11:04:11.55 Forearm.seek 2 + 700 counts @11:04:12.63 SE.reconfigure SEpick @11:04:12.71 SE.seek beamBreak @11:04:14.65 SE.configure SEconfig @11:04:14.82 Elbow.reconfigure LimpElbow @11:04:14.88 Forearm.reconfigure LooseForearm @11:04:14.92 Forearm.seek 2 @11:04:15.23 Elbow.hold @11:04:15.30 Elbow.reconfigure TightElbow @11:04:15.41 SE.seek 12.07 Pucks below Top Plate @11:04:16.03 Forearm.force -20 @11:04:16.07 Forearm.seek 2 + 319 counts @11:04:16.41 Forearm.reconfigure TightForearm @11:04:16.45 Forearm.seek 2 + 700 counts @11:04:16.80 SE.seek 13.07 Pucks below Top Plate @11:04:17.46 Forearm.reconfigure LooseForearm @11:04:17.51 Forearm.seek :retract @11:04:18.43 Elbow.seek CC @11:04:20.13 Elbow.reconfigure LooseElbow @11:04:20.24 Forearm.seek CC @11:04:21.29 Hand.close @11:04:22.02 CC.seek :open @11:04:26.50 Forearm.seek :retract @11:04:27.51 Elbow.reconfigure TightElbow @11:04:27.56 Elbow.seek 2 @11:04:29.47 Elbow.reconfigure LimpElbow @11:04:29.52 Forearm.seek 2 @11:04:30.52 Elbow.hold @11:04:30.59 Elbow.reconfigure TightElbow @11:04:30.64 Hand.open @11:04:32.06 Forearm.force -20 @11:04:32.11 Forearm.seek 2 + 345 counts @11:04:32.43 Forearm.reconfigure TightForearm @11:04:32.48 Forearm.seek 2 + 700 counts @11:04:32.79 SE.seek :down @11:04:33.94
Forearm.reconfigure LooseForearm @11:04:33.99 Forearm.seek :retract @11:04:34.34 SE.coast @11:04:34.90
SC.coast @11:04:34.94 PSV.dial :air @11:04:36.21 PS.seek 0.499ml @11:04:42.07 delay 3 seconds @11:04:45.09 PSV.dial :mixloop @11:04:45.56 PRV.dial :air @11:04:46.06 delay 3 seconds @11:04:49.08 PRV.dial CBV @11:04:49.57 CBV.dial AIV @11:04:50.08 PRV.dial between CBV and air @11:04:50.43 PRV.dial :air @11:04:50.83 PS.reconfigure PSslow @11:04:50.88 PS.seek 0.599ml @11:04:53.42 PRV.dial :waste2 @11:04:54.21 PS.reconfigure PSconfig @11:04:54.26 PS.seek :empty @11:05:01.23 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:05:01.30 Hand.open @11:05:01.66 Elbow.seek FlushPuck::Garage @11:05:03.70 Elbow.reconfigure LooseElbow @11:05:03.81 Forearm.seek :garage @11:05:04.79 Hand.close @11:05:05.53 Forearm.seek :retract @11:05:06.54 Elbow.reconfigure TightElbow @11:05:06.59 Elbow.seek CC @11:05:09.51 Elbow.reconfigure LooseElbow @11:05:09.62 Forearm.reconfigure TightForearm @11:05:09.66 Forearm.seek CC @11:05:10.98 CC.seek :closed @11:05:15.25 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.10 @11:05:15.32 Hand.open @11:05:15.97 Forearm.reconfigure LooseForearm @11:05:16.02 Forearm.seek :retract @11:05:17.09 Clearing PML for modified lysate @11:05:17.10 CTV.dial :air,avoiding: :intake @11:05:17.11 PRV.dial :waste2 @11:05:17.13 PTV.dial :PRV,avoiding: :puck @11:05:17.92 delay 2 seconds @11:05:19.94 PTV.dial :PRVmixing,avoiding: :puck @11:05:21.40 delay 2 seconds @11:05:23.42 PSV.dial :PML @11:05:23.43 delay 2 seconds @11:05:25.46 PRV.dial :air @11:05:26.26 delay 2 seconds @11:05:28.34 PS.seek 1.499ml @11:05:44.39 delay 5 seconds @11:05:49.42 PS.seek 2.5ml @11:06:00.36 PSV.dial :air @11:06:00.85 PS.seek 0ml @11:06:27.01 mixing lysate with speDil @11:06:27.02 PSV.dial :PML @11:06:27.50 CBV.dial :PRV @11:06:27.51 PRV.dial :air @11:06:27.53 delay 2 seconds @11:06:29.55 PRV.dial :speDil @11:06:30.04 delay 2 seconds @11:06:32.13 PS.reconfigure PSslow1 @11:06:32.18 PS.seek 0.225ml @11:06:55.64 delay 5 seconds @11:07:00.67 PRV.dial CBV @11:07:01.48 delay 2 seconds @11:07:03.56 PS.seek 0.475ml @11:07:29.64 delay 5 seconds @11:07:34.67 PRV.dial :air @11:07:35.19 delay 2 seconds @11:07:37.22 PS.seek 0.965ml @11:08:27.17 delay 10 seconds @11:08:37.20 PRV.dial CBV @11:08:37.70 CTV.dial :air,avoiding: :intake @11:08:37.71 PSV.dial :air @11:08:38.19 PS.reconfigure PSconfig @11:08:38.25 PS.seek 2.5ml @11:08:54.55 delay 3 seconds @11:08:57.57 PSV.dial PTV @11:08:58.36 PTV.dial :PRV,avoiding: :puck @11:08:59.17 PS.seek 0ml @11:09:25.27 PSV.dial :air @11:09:26.07 PS.seek 2.5ml @11:09:52.17 delay 3 seconds @11:09:55.19 PSV.dial PTV @11:09:55.98 PTV.dial :PRV,avoiding: :puck @11:09:56.00 PS.seek 0ml @11:10:22.11 Pushing modified lysate into AIV Injection Loop @11:10:22.12 CBV.dial AIV @11:10:22.13 AIV.dial :bypass @11:10:22.15 PRV.dial CBV @11:10:22.16 PSV.dial :air @11:10:22.95 PS.seek 2.5ml @11:10:49.17 delay 3 seconds @11:10:52.19 PSV.dial :PML @11:10:52.66 PTV.dial :PRVmixing,avoiding: :puck @11:10:53.54 PS.reconfigure PSslow1 @11:10:53.59 PS.seek 1.74ml @11:12:10.92 delay 5 seconds @11:12:15.95 AIV.dial :sample @11:12:16.58 AWV.select :waste @11:12:16.75 PS.reconfigure PSslow2 @11:12:16.80 PS.seek 1.22ml @11:14:03.64 delay 15 seconds @11:14:18.67 AIV.dial :bypass @11:14:19.28 PS.reconfigure PSconfig @11:14:19.33 PS.seek 0ml @11:14:32.52 PSV.dial :air @11:14:33.00 PS.seek 2.5ml @11:14:59.08 PSV.dial :PML @11:14:59.56 PS.seek 0ml @11:15:25.71 AIV.dial :coilToMFB @11:15:25.72 AWV.select :air @11:15:25.89 Extracting Nucleic Acid from AIV @11:15:25.92
Beginning samplePassD @11:15:25.99 SPE.hold 2:46:40,55C @11:15:26.04 ASV.dial :SPEdelivery @11:15:26.12
AWV.select :air @11:15:26.13 AIV.dial :bypass @11:15:26.73 PSV.dial PSR @11:15:26.87 delay 20 seconds @11:15:28.02
Pulling 1.5ml of PSR.killMFB @11:15:28.03 PSR.select :killMFB @11:15:28.16 PS.seek 1.5ml @11:15:44.18 delay 5 seconds @11:15:46.90 primeLysateINT @11:15:46.91 AWV.select :air @11:15:46.92 AIV.dial :coilToMFB @11:15:46.94 ATV.dial AIV @11:15:48.77 AS.reconfigure ASslow1 @11:15:48.82 AS.seek 0.299ml @11:15:49.20
PSV.dial :waste1 @11:15:49.72 PS.seek 0ml @11:16:05.74 PSV.dial PSR @11:16:06.35 PSR.select :air @11:16:06.48 PS.seek 2.5ml @11:16:32.66 PRV.dial CBV @11:16:32.68
CBV.dial PRV @11:16:32.70 PSV.dial :mixloop @11:16:33.85 CTV.dial :air,avoiding: :intake @11:16:33.86 PTV.dial :PRVmixing @11:16:33.88 PS.seek 0ml @11:16:49.45 delay 10 seconds @11:16:59.48 ATV.dial :air2 @11:17:00.05
PSV.dial PSR @11:17:00.33 AS.seek 0.329ml @11:17:01.25
Pulling 1.5ml of PSR.flush PSR.select :flush @11:17:01.38 PS.seek 1.5ml @11:17:06.65 ATV.dial :waste2 @11:17:07.21 AS.reconfigure ASnorm @11:17:07.26 AS.seek :empty @11:17:17.46
delay 5 seconds @11:17:22.49 PSV.dial :waste1 @11:17:23.02 PS.seek 0ml @11:17:24.26 Lysate primed! @11:17:24.27 AFV.select :holdingCoil @11:17:24.28 ATV.dial :air2 @11:17:24.92 AS.reconfigure ASslow1 @11:17:24.97 AS.seek 0.069ml @11:17:39.12
PSV.dial PSR @11:17:39.37 delay 3 seconds @11:17:39.63
PSR.select :air @11:17:39.76 PS.seek 2.5ml @11:17:42.40 ATV.dial :AIV @11:17:43.27 AS.seek 0.469ml @11:18:05.84 PRV.dial CBV @11:18:05.87
CBV.dial PRV @11:18:05.88 PSV.dial :mixloop @11:18:07.02 CTV.dial :air,avoiding: :intake @11:18:07.03 PTV.dial :PRVmixing @11:18:07.05 PS.seek 0ml @11:18:33.15 PSV.dial PSR @11:18:34.35 Pulling 1.5ml of PSR.flush @11:18:34.36 PSR.select :flush @11:18:34.49 PS.seek 1.5ml @11:18:50.59 delay 5 seconds @11:18:55.62 PSV.dial :waste1 @11:18:56.13 PS.seek 0ml @11:19:03.98 delay 5 seconds @11:19:09.07 Solid Phase Extraction Heater at 45.1C @11:19:09.08 ATV.dial ASV @11:19:09.50 ASV.dial :SPEdelivery @11:19:09.58 AS.seek 0.041ml @11:19:12.12
PSV.dial PSR @11:19:12.63 PSR.select :air @11:19:12.75 PS.seek 2.5ml @11:19:38.93 PRV.dial CBV @11:19:38.96
CBV.dial PRV @11:19:38.97 PSV.dial :mixloop @11:19:40.20 CTV.dial :air,avoiding: :intake @11:19:40.21 PTV.dial :PRVmixing @11:19:40.23 PS.seek 0ml @11:20:06.43 PSV.dial PSR @11:20:08.26 Pulling 1.5ml of PSR.flush @11:20:08.27 PSR.select :flush @11:20:08.40 PS.seek 1.5ml @11:20:24.53 delay 5 seconds @11:20:29.56 PSV.dial :waste1 @11:20:30.07 PS.seek 0ml @11:20:35.92 delay 10 seconds @11:20:45.95 rinseLysateLineINT @11:20:45.96 AFV.select :carrierSolution @11:20:46.06
PSV.dial PSR @11:20:46.12 delay 2 seconds @11:20:46.56
PSR.select :air @11:20:46.69 PS.seek 2.5ml @11:20:48.14 AS.reconfigure ASconfig @11:20:48.19 AS.seek 1ml @11:21:12.87 PRV.dial CBV @11:21:12.90
CBV.dial PRV @11:21:12.92 PSV.dial :mixloop @11:21:14.03 CTV.dial :air,avoiding: :intake @11:21:14.04 PTV.dial :PRVmixing @11:21:14.06 PS.reconfigure PSslow @11:21:14.11 PS.seek 1.2ml @11:21:40.87 PSV.dial :waste1 @11:21:42.29 PS.reconfigure PSconfig @11:21:42.34 PS.seek 0ml @11:21:43.58 delay 1 second @11:21:44.60 AFV.select :holdingCoil @11:21:44.70 delay 1 second @11:21:45.72 ATV.dial AIV @11:21:46.29 AIV.dial :coilToMFB @11:21:46.31 AWV.select :waste @11:21:46.43 AS.reconfigure ASslow0 @11:21:46.47 AS.seek 0ml @11:21:55.28
PSV.dial :air @11:21:56.52 PS.seek 2.5ml @11:22:22.68 PSV.dial :mixloop @11:22:23.18 PS.reconfigure PSslow @11:22:23.22 PS.seek 0ml @11:23:14.31 PSV.dial :air @11:23:14.81 PS.reconfigure PSconfig @11:23:14.86 PS.seek 2.5ml @11:23:27.36 ATV.dial :air2 @11:23:28.04 AS.reconfigure ASslow1 @11:23:28.09 AS.seek 0.05ml @11:23:38.45 ATV.dial :AIV @11:23:39.26 AS.seek 0.01ml @11:23:40.97
PSV.dial :mixloop @11:23:41.47 PS.reconfigure PSslow @11:23:41.52 PS.seek 0ml @11:23:47.59 ATV.dial :waste @11:23:49.19 AS.seek :empty @11:23:51.62 ethanol wash @11:23:51.63 ATV.dial :air2 @11:23:52.88 AS.reconfigure ASslow2 @11:23:52.93 AS.seek 0.009ml @11:24:03.22 delay 3 seconds @11:24:06.25 Pulling 0.4ml of Ethanol Wash @11:24:06.26 ATV.dial :ethanol @11:24:07.07 AS.reconfigure ASslow1 @11:24:07.12 AS.seek 0.409ml @11:24:32.69
Cleaning Collection Syringe @11:24:32.70 CSV.dial CSR @11:24:33.24 Pulling 4ml of CSR.flush @11:24:33.25 CSR.select :flush @11:24:33.38 CS.seek 4ml @11:24:44.59 delay 5 seconds @11:24:49.62 CSR.select :air @11:24:49.75 CS.seek 10ml @11:25:06.00 CSV.dial CTV @11:25:06.04
CTV.dial CSV @11:25:07.54 CBV.dial :waste1 @11:25:08.05 CS.seek 0ml @11:25:27.81 delay 3 seconds @11:25:30.84 ATV.dial :air1 @11:25:31.23 delay 3 seconds @11:25:34.32 AS.seek 0.434ml @11:25:34.78
CSV.dial CSR @11:25:35.34 Pulling 4ml of CSR.flush @11:25:35.35 CSR.select :flush @11:25:35.48 CS.seek 4ml @11:25:40.23 delay 3 seconds @11:25:43.29 ATV.dial :ethanol @11:25:43.90 AS.seek 0.419ml @11:25:46.72
delay 5 seconds @11:25:47.19 delay 3 seconds @11:25:50.21 ATV.dial ASV @11:25:51.52 AS.seek 0.092ml @11:25:51.74
CSR.select :air @11:25:51.87 CS.seek 10ml @11:26:08.25 CSV.dial CTV @11:26:08.28
CTV.dial CSV @11:26:08.80 CBV.dial :waste1 @11:26:08.82 CS.seek 0ml @11:26:35.35 Garaging FlushPuck in Collection Clamp @11:26:35.43 Elbow.reconfigure TightElbow @11:26:35.48 Elbow.seek CC @11:26:35.73 Elbow.reconfigure LooseElbow @11:26:35.83 Forearm.seek CC @11:26:36.83 Hand.close @11:26:37.44 CC.seek :open @11:26:41.82 Forearm.seek :retract @11:26:42.89 Elbow.reconfigure TightElbow @11:26:42.94 Elbow.seek :garage @11:26:46.12 Elbow.reconfigure LooseElbow @11:26:46.23 Forearm.reconfigure TightForearm @11:26:46.28 Forearm.seek :garage @11:26:47.44 Hand.open @11:26:48.13 Forearm.reconfigure LooseForearm @11:26:48.18 Forearm.seek :retract @11:26:49.22 Whole Cell Archive sampling 1500ml @11:26:49.24 Loading wcr puck from tube 3 into Collection Clamp @11:26:49.58 SC.reconfigure SCtight @11:26:49.63 SC.seek 3 @11:26:51.80 SC.reconfigure SCloose @11:26:52.03 Elbow.reconfigure TightElbow @11:26:52.08 Elbow.seek 3 @11:26:54.27 Forearm.reconfigure TightForearm @11:26:54.32 Forearm.seek 3 + 700 counts @11:26:55.37 SE.reconfigure SEpick @11:26:55.45 SE.seek beamBreak @11:26:57.53 delay 3 seconds @11:26:57.94 SE.configure SEconfig @11:26:58.11 Elbow.seek 3 @11:26:58.41 Forearm.seek 3 + 300 counts @11:26:58.66 Forearm.reconfigure LooseForearm @11:26:58.71 Hand.close @11:26:59.34 SE.seek :down @11:26:59.58 Forearm.seek :retract @11:27:00.52 Elbow.seek CC @11:27:00.59 ATV.dial :waste2 @11:27:01.33 AS.reconfigure ASconfig @11:27:01.38 AS.seek :empty @11:27:02.17 SE.coast @11:27:02.28 Elbow.reconfigure LooseElbow @11:27:02.40 Forearm.reconfigure TightForearm @11:27:02.45 Forearm.seek CC @11:27:03.81 CC.seek :closed @11:27:07.75 ATV.dial :air2 @11:27:08.06 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.26 @11:27:08.08 Hand.open @11:27:08.16 AS.reconfigure ASslow1 @11:27:08.21 AS.seek 1ml @11:27:08.76 Forearm.reconfigure LooseForearm @11:27:08.81 Forearm.seek :retract @11:27:09.81 SC.coast @11:27:09.85 collectSample 1500ml w/bubble point of 55psi @11:27:09.87 Priming 7ml @11:27:09.88 Intake.select :close @11:27:09.89 Exhaust.select :close @11:27:09.90 SS.hold @11:27:10.14 Calibrating pressure sensors @11:27:10.15 CTV.dial :air @11:27:11.58 SSV.dial :spit,avoiding: :exhaust @11:27:11.60 SS.reconfigure SSfast @11:27:11.65 SS.seek 5ml @11:27:14.34 SSV.dial CBV @11:27:14.37 CBV.dial SSV @11:27:15.91 delay 3.8 seconds @11:27:19.86 SSV.dial :spit @11:27:21.30 SS.seek 0ml @11:27:23.95 SSV.dial CBV @11:27:25.38 CTV.dial :intake @11:27:26.20 SSV.dial CBV @11:27:26.23 CBV.dial SSV @11:27:26.25 Allowing 40s for 7.0ml of remaining 7.0ml @11:27:26.26 Intake.select :open @11:27:28.45 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:27:28.47 SS.reconfigure SSinhale.with maxSpeed: 150 @11:27:28.51 SS.seek 7ml @11:27:32.48 Vacuum too high -- waiting up to 40s @11:27:36.09 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:27:36.11 SS.reconfigure SSinhale.with maxSpeed: 135 @11:27:36.16 SS.seek 7ml @11:27:38.98 Stroke completed -- waiting for pressure to equalize ... @11:27:45.46 Intake.select :close @11:27:45.62 SSV.dial :exhaust @11:27:46.46 Exhaust.select :open @11:27:46.58 SS.reconfigure SSexhale @11:27:46.63 SS.seek 0ml @11:27:50.45 Exhaust.select :close @11:27:50.56 Sampling 1500.0ml @11:27:50.58 Intake.select :close @11:27:50.59 Exhaust.select :close @11:27:50.60 SS.reconfigure SSconfig @11:27:50.65 SS.hold @11:27:50.88 CTV.dial :intake @11:27:50.90 SSV.dial CBV @11:27:50.94 CBV.dial SSV @11:27:51.77 Allowing 130s for 25.0ml of remaining 1500.0ml @11:27:51.79 Intake.select :open @11:27:53.97 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:27:53.99 SS.reconfigure SSinhale.with maxSpeed: 135 @11:27:54.03 SS.seek 25ml @11:27:57.82 Vacuum too high -- waiting up to 40s @11:28:00.46 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:28:00.48 SS.reconfigure SSinhale.with maxSpeed: 122 @11:28:00.54 SS.seek 25ml @11:28:03.61 Vacuum too high -- waiting up to 40s @11:28:05.97 SS.reconfigure SSinhale.with maxSpeed: 110 @11:28:06.03 SS.seek 25ml @11:28:08.84 Vacuum too high -- waiting up to 40s @11:28:11.22 SS.reconfigure SSinhale.with maxSpeed: 99 @11:28:11.27 SS.seek 25ml @11:28:15.44 Vacuum too high -- waiting up to 40s @11:28:17.66 SS.reconfigure SSinhale.with maxSpeed: 89 @11:28:17.71 SS.seek 25ml @11:28:20.17 Vacuum too high -- waiting up to 40s @11:28:22.05 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:28:22.07 SS.reconfigure SSinhale.with maxSpeed: 80 @11:28:22.12 SS.seek 25ml @11:28:24.80 Vacuum too high -- waiting up to 40s @11:28:26.92 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:28:26.93 SS.reconfigure SSinhale.with maxSpeed: 72 @11:28:26.98 SS.seek 25ml @11:28:29.98 Vacuum too high -- waiting up to 40s @11:28:31.84 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:28:31.86 SS.reconfigure SSinhale.with maxSpeed: 65 @11:28:31.91 SS.seek 25ml @11:28:35.45 Vacuum too high -- waiting up to 40s @11:28:37.56 SS.reconfigure SSinhale.with maxSpeed: 59 @11:28:37.61 SS.seek 25ml @11:28:39.62 Stroke completed -- waiting for pressure to equalize ... @11:28:44.19 Intake.select :close @11:28:44.35 SSV.dial :exhaust @11:28:45.18 Exhaust.select :open @11:28:45.30 SS.reconfigure SSexhale @11:28:45.35 SS.seek 0ml @11:28:54.21 Exhaust.select :close @11:28:54.32 SSV.dial CBV @11:28:54.36 CBV.dial SSV @11:28:55.20 Allowing 130s for 25.0ml of remaining 1475.0ml @11:28:55.21 Intake.select :open @11:28:57.40 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:28:57.42 SS.reconfigure SSinhale.with maxSpeed: 59 @11:28:57.47 SS.seek 25ml @11:29:03.98 Vacuum too high -- waiting up to 40s @11:29:05.60 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:29:05.62 SS.reconfigure SSinhale.with maxSpeed: 53 @11:29:05.66 SS.seek 25ml @11:29:12.14 Vacuum too high -- waiting up to 40s @11:29:13.74 SS.reconfigure SSinhale.with maxSpeed: 48 @11:29:13.79 SS.seek 25ml @11:29:21.89 Vacuum too high -- waiting up to 40s @11:29:23.49 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:29:23.51 SS.reconfigure SSinhale.with maxSpeed: 43 @11:29:23.57 SS.seek 25ml @11:29:37.20 Vacuum too high -- waiting up to 40s @11:29:38.82 SS.reconfigure SSinhale.with maxSpeed: 39 @11:29:38.87 SS.seek 25ml @11:29:44.24 Stroke completed -- waiting for pressure to equalize ... @11:29:48.11 Intake.select :close @11:29:48.27 SSV.dial :exhaust @11:29:49.10 Exhaust.select :open @11:29:49.22 SS.reconfigure SSexhale @11:29:49.28 SS.seek 0ml @11:29:58.17 Exhaust.select :close @11:29:58.29 SSV.dial CBV @11:29:58.31 CBV.dial SSV @11:29:59.88 Allowing 130s for 25.0ml of remaining 1450.0ml @11:29:59.89 Intake.select :open @11:30:02.08 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:30:02.10 SS.reconfigure SSinhale.with maxSpeed: 39 @11:30:02.14 SS.seek 25ml @11:30:29.77 delay 5 seconds @11:30:34.80 ATV.dial ASV @11:30:35.79 ASV.dial :SPEdelivery @11:30:35.81 AS.seek 0.02ml @11:30:46.55 Vacuum too high -- waiting up to 40s @11:30:48.42 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:30:48.44 SS.reconfigure SSinhale.with maxSpeed: 35 @11:30:48.49 SS.seek 25ml @11:30:50.30 Stroke completed -- waiting for pressure to equalize ... @11:30:53.58 Intake.select :close @11:30:53.74 SSV.dial :exhaust @11:30:54.59 Exhaust.select :open @11:30:54.71 SS.reconfigure SSexhale @11:30:54.76 SS.seek 0ml @11:31:03.95 Exhaust.select :close @11:31:04.07 SSV.dial CBV @11:31:04.09 CBV.dial SSV @11:31:04.95 Allowing 130s for 25.0ml of remaining 1425.0ml @11:31:04.96 Intake.select :open @11:31:07.15 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:31:07.17 SS.reconfigure SSinhale.with maxSpeed: 35 @11:31:07.21 SS.seek 25ml @11:31:57.63 Stroke completed -- waiting for pressure to equalize ... @11:32:02.12 Intake.select :close @11:32:02.28 SSV.dial :exhaust @11:32:03.13 Exhaust.select :open @11:32:03.25 SS.reconfigure SSexhale @11:32:03.30 SS.seek 0ml @11:32:12.21 Exhaust.select :close @11:32:12.33 SSV.dial CBV @11:32:12.35 CBV.dial SSV @11:32:13.20 Allowing 130s for 25.0ml of remaining 1400.0ml @11:32:13.21 Intake.select :open @11:32:15.40 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:32:15.42 SS.reconfigure SSinhale.with maxSpeed: 35 @11:32:15.46 SS.seek 25ml @11:33:06.12 Stroke completed -- waiting for pressure to equalize ... @11:33:11.25 Intake.select :close @11:33:12.06 SSV.dial :exhaust @11:33:12.95 Exhaust.select :open @11:33:13.07 SS.reconfigure SSexhale @11:33:13.12 SS.seek 0ml @11:33:21.87 Exhaust.select :close @11:33:21.98 SSV.dial CBV @11:33:22.02 CBV.dial SSV @11:33:22.87 Allowing 130s for 25.0ml of remaining 1375.0ml @11:33:22.89 Intake.select :open @11:33:25.07 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:33:25.09 SS.reconfigure SSinhale.with maxSpeed: 35 @11:33:25.13 SS.seek 25ml @11:33:53.16 delay 5 seconds @11:33:58.19 ATV.dial :air2 @11:33:59.25 AS.seek 0.52ml @11:34:15.82 Stroke completed -- waiting for pressure to equalize ... @11:34:22.27 Intake.select :close @11:34:22.43 SSV.dial :exhaust @11:34:23.28 Exhaust.select :open @11:34:23.40 SS.reconfigure SSexhale @11:34:23.45 SS.seek 0ml @11:34:32.48 Exhaust.select :close @11:34:32.60 SSV.dial CBV @11:34:32.62 CBV.dial SSV @11:34:33.48 Allowing 130s for 25.0ml of remaining 1350.0ml @11:34:33.49 Intake.select :open @11:34:35.68 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:34:35.70 SS.reconfigure SSinhale.with maxSpeed: 35 @11:34:35.74 SS.seek 25ml @11:35:15.61 Vacuum too high -- waiting up to 40s @11:35:17.47 Intake @31.1psia, vacuum from 20.0 to 24.0psi @11:35:17.49 SS.reconfigure SSinhale.with maxSpeed: 32 @11:35:17.54 SS.seek 25ml @11:35:29.19 Stroke completed -- waiting for pressure to equalize ... @11:35:34.30 Intake.select :close @11:35:34.46 SSV.dial :exhaust @11:35:35.29 Exhaust.select :open @11:35:35.41 SS.reconfigure SSexhale @11:35:35.46 SS.seek 0ml @11:35:40.09 delay 5 seconds @11:35:44.62 Exhaust.select :close @11:35:44.74 SSV.dial CBV @11:35:44.76 CBV.dial SSV @11:35:45.11 ATV.dial ASV @11:35:45.64 Allowing 130s for 25.0ml of remaining 1325.0ml @11:35:45.65 Intake.select :open @11:35:46.73 ASV.dial :wasteR @11:35:47.16 AS.seek 0.3ml @11:35:47.84 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:35:47.86 SS.reconfigure SSinhale.with maxSpeed: 32 @11:35:47.91 SS.seek 25ml @11:36:31.65 delay 20 seconds @11:36:42.76 Stroke completed -- waiting for pressure to equalize ... @11:36:49.19 Intake.select :close @11:36:49.35 SSV.dial :exhaust @11:36:50.17 Exhaust.select :open @11:36:50.29 SS.reconfigure SSexhale @11:36:50.34 SS.seek 0ml @11:36:51.68 AS.seek 0.07ml @11:36:59.51 Exhaust.select :close @11:36:59.63 SSV.dial CBV @11:36:59.65 CBV.dial SSV @11:37:00.51 Allowing 130s for 25.0ml of remaining 1300.0ml @11:37:00.52 Intake.select :open @11:37:02.70 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:37:02.72 SS.reconfigure SSinhale.with maxSpeed: 32 @11:37:02.77 SS.seek 25ml @11:37:38.18 delay 20 seconds @11:37:48.45 Vacuum too high -- waiting up to 40s @11:37:50.56 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:37:50.58 SS.reconfigure SSinhale.with maxSpeed: 29 @11:37:50.64 SS.seek 25ml @11:37:58.21 ATV.dial :waste2 @11:37:58.96 AS.reconfigure ASconfig @11:37:59.01 AS.seek :empty @11:38:01.14 Stroke completed -- waiting for pressure to equalize ... @11:38:03.40 Elution @11:38:03.42 ATV.dial :air1 @11:38:04.54 AS.reconfigure ASslow1 @11:38:04.60 AS.seek 0.049ml @11:38:06.87 Intake.select :close @11:38:07.03 SSV.dial :exhaust @11:38:07.87 Exhaust.select :open @11:38:07.99 SS.reconfigure SSexhale @11:38:08.04 SS.seek 0ml @11:38:14.98 delay 3 seconds @11:38:16.96 Exhaust.select :close @11:38:17.08 SSV.dial CBV @11:38:17.10 CBV.dial SSV @11:38:17.96 Allowing 130s for 25.0ml of remaining 1275.0ml @11:38:18.65 Intake.select :open @11:38:18.73 ATV.dial :ElutionWater @11:38:19.42 AS.seek 0.109ml @11:38:20.85 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:38:20.87 SS.reconfigure SSinhale.with maxSpeed: 29 @11:38:20.93 SS.seek 25ml @11:38:31.77 delay 3 seconds @11:38:34.80 ATV.dial ASV,via: :air2 @11:38:36.88 ASV.dial :SPEelution @11:38:37.49 AFV.select :carrierSolution @11:38:37.66 AS.reconfigure ASnorm @11:38:37.71 AS.seek 0.759ml @11:39:10.79 delay 3 seconds @11:39:13.82 AFV.select :holdingCoil @11:39:13.97 AS.reconfigure ASslow1 @11:39:14.03 AS.seek 0.474ml @11:39:21.76 Stroke completed -- waiting for pressure to equalize ... @11:39:28.83 Intake.select :close @11:39:28.99 SSV.dial :exhaust @11:39:29.82 Exhaust.select :open @11:39:29.94 SS.reconfigure SSexhale @11:39:29.99 SS.seek 0ml @11:39:39.22 Exhaust.select :close @11:39:39.34 SSV.dial CBV @11:39:39.36 CBV.dial SSV @11:39:40.22 Allowing 130s for 25.0ml of remaining 1250.0ml @11:39:40.23 Intake.select :open @11:39:42.42 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:39:42.44 SS.reconfigure SSinhale.with maxSpeed: 29 @11:39:42.48 SS.seek 25ml @11:40:11.63 delay 5 seconds @11:40:16.72 AS.reconfigure ASslow2 @11:40:16.77 AS.seek 0.439ml @11:40:33.61 Vacuum too high -- waiting up to 40s @11:40:36.27 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:40:36.29 SS.reconfigure SSinhale.with maxSpeed: 26 @11:40:36.33 SS.seek 25ml @11:40:47.11 Stroke completed -- waiting for pressure to equalize ... @11:40:52.17 delay 1:00 @11:40:54.27 Intake.select :close @11:40:54.43 SSV.dial :exhaust @11:40:55.28 Exhaust.select :open @11:40:55.40 SS.reconfigure SSexhale @11:40:55.45 SS.seek 0ml @11:41:04.62 Exhaust.select :close @11:41:04.74 SSV.dial CBV @11:41:04.76 CBV.dial SSV @11:41:05.64 Allowing 130s for 25.0ml of remaining 1225.0ml @11:41:05.65 Intake.select :open @11:41:07.83 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:41:07.85 SS.reconfigure SSinhale.with maxSpeed: 26 @11:41:07.90 SS.seek 25ml @11:41:52.21 pushing eluate to holding coil @11:41:52.29 AS.seek 0.279ml @11:42:15.23 Stroke completed -- waiting for pressure to equalize ... @11:42:24.26 Intake.select :close @11:42:24.42 SSV.dial :exhaust @11:42:25.27 Exhaust.select :open @11:42:25.39 SS.reconfigure SSexhale @11:42:25.44 SS.seek 0ml @11:42:34.50 Exhaust.select :close @11:42:34.62 SSV.dial CBV @11:42:34.64 CBV.dial SSV @11:42:35.50 Allowing 130s for 25.0ml of remaining 1200.0ml @11:42:35.51 Intake.select :open @11:42:37.69 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:42:37.71 SS.reconfigure SSinhale.with maxSpeed: 26 @11:42:37.76 SS.seek 25ml @11:43:21.89 Vacuum too high -- waiting up to 40s @11:43:24.74 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:43:24.76 SS.reconfigure SSinhale.with maxSpeed: 23 @11:43:24.81 SS.seek 25ml @11:43:51.30 Stroke completed -- waiting for pressure to equalize ... @11:44:00.27 Intake.select :close @11:44:00.43 SSV.dial :exhaust @11:44:01.26 Exhaust.select :open @11:44:01.38 SS.reconfigure SSexhale @11:44:01.44 SS.seek 0ml @11:44:10.62 Exhaust.select :close @11:44:10.74 SSV.dial CBV @11:44:10.77 CBV.dial SSV @11:44:11.61 Allowing 130s for 25.0ml of remaining 1175.0ml @11:44:11.62 Intake.select :open @11:44:13.81 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:44:13.83 SS.reconfigure SSinhale.with maxSpeed: 23 @11:44:13.87 SS.seek 25ml @11:44:33.29 delay 30 seconds @11:45:03.32 ATV.dial :waste,via: :air3 @11:45:05.00 AS.reconfigure ASconfig @11:45:05.05 AS.seek :empty @11:45:17.11 Vacuum too high -- waiting up to 40s @11:45:20.26 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:45:20.28 SS.reconfigure SSinhale.with maxSpeed: 21 @11:45:20.33 SS.seek 25ml @11:45:21.45 Parking Bleach in LysateSource line and SPEcolumn @11:45:21.46 ATV.dial :air3 @11:45:22.31 AS.reconfigure ASslow1 @11:45:22.36 AS.seek 0.029ml @11:45:28.78 ATV.dial :ElutionWater @11:45:29.40 AS.seek 0.059ml @11:45:34.53 Stroke completed -- waiting for pressure to equalize ... @11:45:35.74 ATV.dial :air3 @11:45:36.36 AS.seek 0.069ml @11:45:38.62 delay 2 seconds @11:45:40.64 ATV.dial :bleach @11:45:41.11 AS.seek 0.769ml @11:45:43.54 Intake.select :close @11:45:43.71 SSV.dial :exhaust @11:45:45.27 Exhaust.select :open @11:45:45.39 SS.reconfigure SSexhale @11:45:45.47 SS.seek 0ml @11:45:54.51 Exhaust.select :close @11:45:54.62 SSV.dial CBV @11:45:54.66 CBV.dial SSV @11:45:55.50 Allowing 130s for 25.0ml of remaining 1150.0ml @11:45:55.51 Intake.select :open @11:45:57.69 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:45:57.71 SS.reconfigure SSinhale.with maxSpeed: 21 @11:45:57.77 SS.seek 25ml @11:47:07.02 Vacuum too high -- waiting up to 40s @11:47:10.71 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:47:10.73 SS.reconfigure SSinhale.with maxSpeed: 19 @11:47:10.78 SS.seek 25ml @11:47:26.76 Stroke completed -- waiting for pressure to equalize ... @11:47:36.99 Intake.select :close @11:47:37.15 SSV.dial :exhaust @11:47:37.98 Exhaust.select :open @11:47:38.10 SS.reconfigure SSexhale @11:47:38.15 SS.seek 0ml @11:47:47.20 Exhaust.select :close @11:47:47.32 SSV.dial CBV @11:47:47.34 CBV.dial SSV @11:47:48.20 Allowing 130s for 25.0ml of remaining 1125.0ml @11:47:48.21 Intake.select :open @11:47:50.40 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:47:50.42 SS.reconfigure SSinhale.with maxSpeed: 19 @11:47:50.46 SS.seek 25ml @11:48:02.12 delay 3 seconds @11:48:05.15 ATV.dial :air3 @11:48:05.69 delay 3 seconds @11:48:08.78 AS.seek 0.794ml @11:48:14.09 delay 3 seconds @11:48:17.11 ATV.dial :bleach @11:48:17.73 AS.seek 0.779ml @11:48:21.05 delay 3 seconds @11:48:24.08 ATV.dial :AIV @11:48:25.31 AS.reconfigure ASconfig @11:48:25.36 AS.seek 0.015ml @11:49:06.18 Vacuum too high -- waiting up to 40s @11:49:10.12 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:49:10.14 SS.reconfigure SSinhale.with maxSpeed: 17 @11:49:10.18 SS.seek 25ml @11:49:10.31 ATV.dial :waste @11:49:11.78 AS.seek :empty @11:49:12.98 ATV.dial :air3 @11:49:13.65 AS.reconfigure ASslow1 @11:49:13.70 AS.seek 0.009ml @11:49:16.03 delay 2 seconds @11:49:18.05 ATV.dial :bleach @11:49:18.52 AS.seek 0.579ml @11:49:29.09 Stroke completed -- waiting for pressure to equalize ... @11:49:41.21 Intake.select :close @11:49:41.37 SSV.dial :exhaust @11:49:42.20 Exhaust.select :open @11:49:42.32 SS.reconfigure SSexhale @11:49:42.37 SS.seek 0ml @11:49:51.62 Exhaust.select :close @11:49:51.74 SSV.dial CBV @11:49:51.76 CBV.dial SSV @11:49:52.62 Allowing 130s for 25.0ml of remaining 1100.0ml @11:49:52.63 Intake.select :open @11:49:54.82 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:49:54.84 SS.reconfigure SSinhale.with maxSpeed: 17 @11:49:54.88 SS.seek 25ml @11:51:13.41 delay 3 seconds @11:51:15.10 Vacuum too high -- waiting up to 40s @11:51:16.61 ATV.dial :air3 @11:51:17.17 delay 3 seconds @11:51:18.99 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:51:19.01 SS.reconfigure SSinhale.with maxSpeed: 15 @11:51:19.05 SS.seek 25ml @11:51:20.25 AS.seek 0.604ml @11:51:25.56 delay 3 seconds @11:51:28.59 ATV.dial :bleach @11:51:29.21 AS.seek 0.589ml @11:51:32.50 delay 3 seconds @11:51:35.53 ATV.dial :ASV @11:51:36.55 ASV.dial :wasteR @11:51:37.15 AS.seek 0ml @11:51:45.44 Stroke completed -- waiting for pressure to equalize ... @11:51:58.20 Intake.select :close @11:51:58.36 SSV.dial :exhaust @11:51:59.20 Exhaust.select :open @11:51:59.32 SS.reconfigure SSexhale @11:51:59.37 SS.seek 0ml @11:52:08.62 Exhaust.select :close @11:52:08.74 SSV.dial CBV @11:52:08.76 CBV.dial SSV @11:52:09.62 Allowing 130s for 25.0ml of remaining 1075.0ml @11:52:09.63 Intake.select :open @11:52:11.81 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:52:11.83 SS.reconfigure SSinhale.with maxSpeed: 15 @11:52:11.88 SS.seek 25ml @11:53:35.88 delay 1:00 @11:53:52.68 Vacuum too high -- waiting up to 40s @11:53:57.58 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:53:57.60 SS.reconfigure SSinhale.with maxSpeed: 14 @11:53:57.64 SS.seek 25ml @11:54:15.33 Stroke completed -- waiting for pressure to equalize ... @11:54:19.65 SS.reconfigure SSconfig @11:54:19.70 SS.hold @11:54:19.84 Intake.select :close @11:54:20.00 ShallowSampler::Clogged in WCR -- progress too slow after 450.0ml @11:54:20.02 SSV.dial :exhaust @11:54:20.86 Exhaust.select :open @11:54:20.98 SS.reconfigure SSexhale @11:54:21.03 SS.seek 0ml @11:54:30.23 Exhaust.select :close @11:54:30.34 Sampled 450.0ml @11:54:30.36 Drying puck before fixing for 4:00 @11:54:30.37 CBV.dial :waste2b @11:54:31.20 delay 1:00 @11:54:35.91 AFV.select :carrierSolution @11:54:36.01 delay 2 seconds @11:54:38.04 AS.reconfigure ASconfig @11:54:38.09 AS.seek 1ml @11:55:31.22 CSV.dial :air @11:55:31.72 CS.reconfigure CSfast @11:55:31.76 CS.seek 10ml @11:55:35.83 delay 1 second @11:55:36.85 AFV.select :holdingCoil @11:55:36.95 delay 1 second @11:55:37.98 ATV.dial ASV @11:55:37.99 ASV.dial :wasteR @11:55:38.01 AS.reconfigure ASslow1 @11:55:38.05 AS.seek 0ml @11:55:56.69 Pumping wcr puck from tube 3 up to 55psi... @11:55:56.71 CBV.dial SSV @11:55:58.11 CBV.dial between CSV and waste2b @11:55:59.18 CSV.dial between air and CTV @11:55:59.53 CS.seek 7.198ml @11:56:07.23 CTV.dial CSV,avoiding: :air @11:56:07.25 CSV.dial CTV @11:56:08.16 CS.reconfigure CSpressurize @11:56:08.22 CS.seek 0ml @11:56:27.32 CSV.dial :air @11:56:27.82 CS.reconfigure CSfast @11:56:27.88 CS.seek 10ml @11:56:52.89 CSV.dial between air and CTV @11:56:53.25 CS.seek 2.482ml @11:57:12.33 CTV.dial CSV,avoiding: :air @11:57:12.35 CSV.dial CTV @11:57:12.72 CS.reconfigure CSpressurize @11:57:13.46 CS.seek 0ml @11:57:17.56 CBV.dial :waste2b @11:57:17.93 CS.reconfigure CSconfig @11:57:17.99 CS.seek :empty @11:57:21.66 delay 1:00 @11:58:21.69 CTV.dial :air,avoiding: :intake @11:58:23.15 Fix #1 of 2 (with rnal) for 2:00 @11:58:23.17 CSV.dial CSR @11:58:23.72 Pulling 2ml of CSR.rnal @11:58:23.73 CSR.select :rnal @11:58:23.85 CS.seek 2ml @11:58:29.94 delay 5 seconds @11:58:34.97 CSR.select :air @11:58:35.10 CS.reconfigure CSfast @11:58:35.15 CS.seek 10ml @11:58:55.07 Pumping wcr puck from tube 3 up to 55psi... @11:58:55.09 CBV.dial SSV @11:58:56.47 CBV.dial between CSV and waste2b @11:58:57.55 CSV.dial between air and CTV @11:58:58.24 CS.seek 7.159ml @11:58:59.40 delay 20 seconds @11:59:06.02 CTV.dial CSV,avoiding: :air @11:59:06.04 CSV.dial CTV @11:59:07.55 CS.reconfigure CSpressurize @11:59:07.60 CS.seek 0ml @11:59:19.43 ATV.dial :air3 @11:59:20.90 AS.seek 0.59ml @11:59:26.51 CSV.dial :air @11:59:27.02 CS.reconfigure CSfast @11:59:27.07 CS.seek 10ml @11:59:51.77 CSV.dial between air and CTV @11:59:52.13 CS.seek 2.221ml @12:00:11.67 CTV.dial CSV,avoiding: :air @12:00:11.69 CSV.dial CTV @12:00:12.03 CS.reconfigure CSpressurize @12:00:12.09 CS.seek 0ml @12:00:14.34 delay 2:00 @12:01:19.83 delay 3 seconds @12:01:22.86 ATV.dial :ASV @12:01:24.23 ASV.dial :wasteR @12:01:24.31 AS.seek 0.09ml @12:02:14.37 CBV.dial :waste2b @12:02:14.74 CS.reconfigure CSconfig @12:02:14.80 CS.seek :empty @12:02:19.73 delay 1:00 @12:03:05.15 delay 30 seconds @12:03:19.76 CTV.dial :air,avoiding: :intake @12:03:21.21 Fix #2 of 2 (with rnal) for 2:00 @12:03:21.23 CSV.dial CSR @12:03:21.77 Pulling 2ml of CSR.rnal @12:03:21.79 CSR.select :rnal @12:03:21.91 CS.seek 2ml @12:03:28.11 delay 5 seconds @12:03:33.14 CSR.select :air @12:03:33.27 CS.reconfigure CSfast @12:03:33.32 CS.seek 10ml @12:03:35.17 ATV.dial :extSrc @12:03:35.93 delay 3 seconds @12:03:38.96 ATV.dial :ElutionWater @12:03:40.05 AS.seek 0.12ml @12:03:46.38 ATV.dial :air3 @12:03:47.67 AS.seek 0.13ml @12:03:49.98 delay 2 seconds @12:03:52.00 ATV.dial :bleach @12:03:52.47 AS.seek 0.23ml @12:03:53.33 Pumping wcr puck from tube 3 up to 55psi... @12:03:53.35 CBV.dial SSV @12:03:54.74 CBV.dial between CSV and waste2b @12:03:55.82 CSV.dial between air and CTV @12:03:56.49 CS.seek 7.108ml @12:04:04.34 CTV.dial CSV,avoiding: :air @12:04:04.36 CSV.dial CTV @12:04:05.91 CS.reconfigure CSpressurize @12:04:05.96 CS.seek 0ml @12:04:12.84 delay 3 seconds @12:04:15.87 ATV.dial :air3 @12:04:16.41 delay 3 seconds @12:04:19.50 AS.seek 0.255ml @12:04:24.78 CSV.dial :air @12:04:24.80 delay 3 seconds @12:04:25.32 CS.reconfigure CSfast @12:04:25.37 CS.seek 10ml @12:04:27.85 ATV.dial :bleach @12:04:28.45 AS.seek 0.24ml @12:04:31.74 delay 3 seconds @12:04:34.77 ATV.dial :ASV @12:04:35.79 ASV.dial :SPEdelivery @12:04:36.16 AFV.select :carrierSolution @12:04:36.27 delay 2 seconds @12:04:38.30 AS.reconfigure ASconfig @12:04:38.35 AS.seek 0.5ml @12:04:50.08 CSV.dial between air and CTV @12:04:50.43 CS.seek 2.257ml @12:04:53.59 delay 1 second @12:04:54.61 AFV.select :holdingCoil @12:04:54.76 AS.reconfigure ASslow1 @12:04:54.81 AS.seek 0.17ml @12:05:09.90 CTV.dial CSV,avoiding: :air @12:05:09.92 CSV.dial CTV @12:05:10.38 CS.reconfigure CSpressurize @12:05:10.43 CS.seek 0ml @12:05:12.94 delay 2:00 @12:06:01.44 SPE.hold 2:46:40,95C @12:06:01.50 delay 3:00 @12:07:12.97 CBV.dial :waste2b @12:07:13.34 CS.reconfigure CSconfig @12:07:13.39 CS.seek :empty @12:07:18.40 delay 1:00 @12:08:18.43 CTV.dial :air,avoiding: :intake @12:08:20.01 Storing wcr puck in Collection Clamp to tube 2 @12:08:20.20 SC.reconfigure SCtight @12:08:20.26 SC.seek 2 @12:08:22.46 SC.reconfigure SCloose @12:08:22.69 Elbow.reconfigure TightElbow @12:08:22.74 Elbow.seek 2 @12:08:24.62 Forearm.reconfigure TightForearm @12:08:24.67 Forearm.seek 2 + 700 counts @12:08:26.40 SE.reconfigure SEpick @12:08:26.48 SE.seek beamBreak @12:08:28.40 SE.configure SEconfig @12:08:28.57 Elbow.reconfigure LimpElbow @12:08:28.63 Forearm.reconfigure LooseForearm @12:08:28.68 Forearm.seek 2 @12:08:28.96 Elbow.hold @12:08:29.04 Elbow.reconfigure TightElbow @12:08:29.15 SE.seek 13.08 Pucks below Top Plate @12:08:29.64 Forearm.force -20 @12:08:29.68 Forearm.seek 2 + 347 counts @12:08:30.01 Forearm.reconfigure TightForearm @12:08:30.06 Forearm.seek 2 + 700 counts @12:08:30.32 SE.seek 14.08 Pucks below Top Plate @12:08:30.99 Forearm.reconfigure LooseForearm @12:08:31.05 Forearm.seek :retract @12:08:32.01 Elbow.seek CC @12:08:33.72 Elbow.reconfigure LooseElbow @12:08:33.83 Forearm.seek CC @12:08:34.90 Hand.close @12:08:35.64 CC.seek :open @12:08:40.19 Forearm.seek :retract @12:08:41.23 Elbow.reconfigure TightElbow @12:08:41.28 Elbow.seek 2 @12:08:43.26 Elbow.reconfigure LimpElbow @12:08:43.32 Forearm.seek 2 @12:08:44.34 Elbow.hold @12:08:44.42 Elbow.reconfigure TightElbow @12:08:44.47 Hand.open @12:08:45.40 Forearm.force -20 @12:08:45.45 Forearm.seek 2 + 346 counts @12:08:45.78 Forearm.reconfigure TightForearm @12:08:45.84 Forearm.seek 2 + 700 counts @12:08:46.21 SE.seek :down @12:08:47.35 Forearm.reconfigure LooseForearm @12:08:47.40 Forearm.seek :retract @12:08:47.76 SE.coast @12:08:48.36 SC.coast @12:08:48.40 Starting finalPurge @12:08:48.42 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:08:48.50 Hand.open @12:08:49.51 Elbow.seek FlushPuck::Garage @12:08:51.83 Elbow.reconfigure LooseElbow @12:08:51.94 Forearm.seek :garage @12:08:52.95 Hand.close @12:08:53.90 Forearm.seek :retract @12:08:54.92 Elbow.reconfigure TightElbow @12:08:54.98 Elbow.seek CC @12:08:57.97 Elbow.reconfigure LooseElbow @12:08:58.08 Forearm.reconfigure TightForearm @12:08:58.13 Forearm.seek CC @12:08:59.47 CC.seek :closed @12:09:01.54 AFV.select :carrierSolution @12:09:01.64 delay 2 seconds @12:09:03.74
Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.20 @12:09:03.81 AS.reconfigure ASconfig @12:09:03.88 AS.seek 1ml @12:09:03.90
Hand.open @12:09:04.71 Forearm.reconfigure LooseForearm @12:09:04.76 Forearm.seek :retract @12:09:05.78 Flushing the Collection Valve::Manifold @12:09:05.92 CSV.dial CSR @12:09:06.51 Pulling 5ml of CSR.flush @12:09:06.52 CSR.select :flush @12:09:06.64 CS.seek 5ml @12:09:20.46 delay 5 seconds @12:09:25.49 CSR.select :air @12:09:25.66 CS.reconfigure CSfast @12:09:25.70 CS.seek 7.5ml @12:09:32.89 CSV.dial :waste @12:09:34.36 CS.reconfigure CSconfig @12:09:34.41 CS.seek 0ml @12:09:51.98 delay 1 second @12:09:53.00 AFV.select :holdingCoil @12:09:53.10 delay 1 second @12:09:54.13 ASV.dial :wasteD @12:09:54.55
Flushing lower manifold's waste1 port @12:09:54.69 CSV.dial :flush @12:09:55.19 AS.seek 0ml @12:09:55.26
CS.reconfigure CSfast @12:09:55.31 CS.seek 5ml @12:10:08.71 delay 5 seconds @12:10:13.74 CSV.dial :air @12:10:14.31 CS.seek 7.001ml @12:10:20.27 CSV.dial CTV @12:10:20.30
CTV.dial CSV @12:10:21.86 CBV.dial :waste1 @12:10:22.72 CS.seek 0ml @12:10:40.90 Flushing lower manifold's waste2b port @12:10:41.04 CSV.dial :flush @12:10:41.87 CS.seek 5ml @12:10:52.95 Priming Eluate @12:10:52.96 AFV.select :carrierSolution @12:10:53.07 delay 2 seconds @12:10:55.11 AS.seek 0.5ml @12:10:55.15
delay 5 seconds @12:11:00.18 CSV.dial :air @12:11:00.76 CS.seek 7ml @12:11:07.41 CSV.dial CTV @12:11:07.45
CTV.dial CSV @12:11:07.96 CBV.dial :waste2b @12:11:08.83 CS.seek 0ml @12:11:24.18 delay 1 second @12:11:25.20 AFV.select :holdingCoil @12:11:25.30 ATV.dial ASV @12:11:25.31 ASV.dial :SPErecovery @12:11:25.92 Swish #1 of 2 @12:11:26.00 AS.reconfigure ASslow0 @12:11:26.05 AS.seek 0.6ml @12:11:27.02
Flushing upper manifold @12:11:27.16 CSV.dial :flush @12:11:28.02 CS.seek 5ml @12:11:36.50 AS.seek 0.5ml @12:11:41.24
delay 5 seconds @12:11:46.27 CSV.dial :air @12:11:46.86 CS.seek 7ml @12:11:46.91 Swish #2 of 2 @12:11:47.00 AS.seek 0.6ml @12:11:52.68 CSV.dial CBV @12:11:52.71
CBV.dial CSV @12:11:53.93 CTV.dial :air @12:11:55.44 CS.seek 0ml @12:11:57.41 AS.seek 0.5ml @12:12:07.76 delay 3 seconds @12:12:10.85 AS.reconfigure ASslow1 @12:12:10.90 AS.seek 0.684ml @12:12:13.49
delay 7 seconds @12:12:20.53 CS.seek 3ml @12:12:28.84 CSV.dial :waste @12:12:29.35 CS.seek 0ml @12:12:37.78 CSV.dial :flush @12:12:38.28 CS.seek 3ml @12:12:46.84 delay 5 seconds @12:12:48.20 delay 3 seconds @12:12:51.23 Eluate primed @12:12:51.24 ATV.dial :waste2 @12:12:51.88 AS.reconfigure ASnorm @12:12:51.93
CSV.dial :air @12:12:51.95 AS.seek 0ml @12:12:52.56
CS.seek 5ml @12:12:58.40 CSV.dial :air @12:12:58.42 CS.seek 0ml @12:13:11.65 delay 7 seconds @12:13:18.69 CS.seek 3ml @12:13:26.78 SPE.coast @12:13:26.87 ASV.dial :SPErecovery @12:13:26.89 Setting up PCR for OM -- writing /var/log/esp/OM20may2412h400ml.pcr @12:13:26.90 Building PCR Slug from ASV with MM3 mix, PP5 primer @12:13:26.92 Priming with carrier @12:13:26.93 ATV.dial ARV @12:13:27.09
CSV.dial :waste @12:13:27.87 ARV.dial :carrier @12:13:27.96
CS.seek 0ml @12:13:28.25 AS.seek 0.5ml @12:13:36.06
Disinfecting external Intake with mfbkill @12:13:36.20 CSV.dial CSR @12:13:37.67 Pulling 8ml of CSR.mfbkill @12:13:37.68 CSR.select :mfbkill @12:13:37.80 CS.seek 8ml @12:13:53.76 AFV.select :carrierSolution @12:13:53.87 delay 2 seconds @12:13:55.90 AS.reconfigure ASconfig @12:13:55.95 AS.seek 1ml @12:13:57.74
delay 5 seconds @12:14:02.77 CSR.select :air @12:14:02.89 CS.seek 10ml @12:14:08.59 CSV.dial CBV @12:14:08.62
CBV.dial CSV @12:14:09.73 CTV.dial :intake @12:14:10.54 Intake.select :open @12:14:10.66 CS.seek 2ml @12:14:24.98 delay 1 second @12:14:26.00 AFV.select :holdingCoil @12:14:26.10 delay 1 second @12:14:27.13 ATV.dial :waste @12:14:28.59 AS.reconfigure ASnorm @12:14:29.36 AS.seek :empty @12:14:32.18
Intake.select :close @12:14:32.29 CTV.dial :air @12:14:33.12 CS.reconfigure CSconfig @12:14:33.17 CS.seek 0ml @12:14:39.28 Flushing lower manifold's waste1 port @12:14:39.29 CSV.dial CSR @12:14:40.49 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @12:14:40.62 CS.seek 4ml @12:14:51.99 delay 5 seconds @12:14:57.02 CSR.select :air @12:14:57.15 CS.reconfigure CSfast @12:14:57.19 CS.seek 10ml @12:15:12.28 CSV.dial CTV @12:15:12.31
CTV.dial CSV @12:15:13.84 CBV.dial :waste1 @12:15:14.35 CS.seek 0ml @12:15:20.12 AFV.select :carrierSolution @12:15:20.23 delay 2 seconds @12:15:22.26 AS.reconfigure ASconfig @12:15:22.31 AS.seek 0.5ml @12:15:39.18
Flushing lower manifold's waste2b port @12:15:39.19 CSV.dial CSR @12:15:39.75 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @12:15:39.88 CS.reconfigure CSconfig @12:15:39.93 CS.seek 4ml @12:15:51.13 delay 5 seconds @12:15:51.41 delay 1 second @12:15:52.43 AFV.select :holdingCoil @12:15:52.53 Building Slug @12:15:52.55 ATV.dial :ARV @12:15:54.11 delay 2 seconds @12:15:56.14 ARV.dial :air1 @12:15:56.19
CSR.select :air @12:15:56.32 CS.reconfigure CSfast @12:15:56.36 CS.seek 10ml @12:15:56.53 delay 2 seconds @12:15:58.62 AS.reconfigure ASslow2 @12:15:58.67 AS.seek 0.506ml @12:16:04.97 delay 2 seconds @12:16:07.00 ARV.dial :MM3 @12:16:08.00 delay 2 seconds @12:16:10.03 Adding MM3 @12:16:10.12 AS.seek 0.524ml @12:16:11.46 CSV.dial CTV @12:16:11.50
CTV.dial CSV @12:16:12.01 CBV.dial :waste2b @12:16:12.85 CS.seek 0ml @12:16:28.44 delay 25 seconds @12:16:37.53
Killing upper manifold @12:16:37.54 CSV.dial CSR @12:16:38.10 Pulling 3.999ml of CSR.mfbkill @12:16:38.11 CSR.select :mfbkill @12:16:38.24 CS.reconfigure CSconfig @12:16:38.29 CS.seek 4ml @12:16:49.43 delay 5 seconds @12:16:53.47 ARV.dial :PP5 @12:16:53.86 Adding PP5 @12:16:53.88 delay 2 seconds @12:16:54.45
CSR.select :air @12:16:54.58 CS.reconfigure CSfast @12:16:54.62 CS.seek 10ml @12:16:55.96 AS.seek 0.53ml @12:17:02.22 delay 10 seconds @12:17:09.75 CSV.dial CBV @12:17:09.78
CBV.dial CSV @12:17:11.04 CTV.dial :air @12:17:12.24 ARV.dial :air3 @12:17:12.48
CS.seek 0ml @12:17:12.62 adding ARV air @12:17:12.64 delay 2 seconds @12:17:14.72 AS.seek 0.536ml @12:17:21.16 delay 5 seconds @12:17:26.19 ARV.dial :carrier @12:17:27.41 delay 7 seconds @12:17:34.51 AS.seek 0.594ml @12:17:36.94
delay 7 seconds @12:17:43.98 CS.seek 3ml @12:17:52.03 CSV.dial :waste @12:17:52.54 CS.seek 0ml @12:18:01.50 Flushing upper manifold @12:18:01.64 CSV.dial :flush @12:18:02.13 CS.seek 5ml @12:18:14.87 delay 5 seconds @12:18:19.90 CSV.dial :air @12:18:20.58 CS.seek 7ml @12:18:26.28 CSV.dial CBV @12:18:26.31
CBV.dial CSV @12:18:27.42 CTV.dial :air @12:18:27.44 CS.seek 0ml @12:18:32.98 delay 5 seconds @12:18:38.01 ATV.dial :ASV @12:18:38.39 delay 2 seconds @12:18:40.48 AS.seek 0.6ml @12:18:45.06
delay 7 seconds @12:18:46.78 delay 4 seconds @12:18:50.81 ATV.dial ARV @12:18:51.33 delay 2 seconds @12:18:52.09
CS.seek 3ml @12:18:53.36 ARV.dial :carrier @12:18:53.38 delay 2 seconds @12:18:55.46 AS.seek 0.65ml @12:19:00.09
CSV.dial :waste @12:19:00.59 CS.seek 0ml @12:19:08.94 CSV.dial :flush @12:19:09.43 CS.seek 3ml @12:19:17.56 delay 5 seconds @12:19:22.59 CSV.dial :air @12:19:23.27 CS.seek 5ml @12:19:28.94 CSV.dial :air @12:19:28.96 CS.seek 0ml @12:19:41.66 delay 7 seconds @12:19:46.06 delay 4 seconds @12:19:48.69
CS.seek 3ml @12:19:50.08 ATV.dial PCR @12:19:50.69 ASV.dial :waste1 @12:19:51.45 ARV.dial :air1 @12:19:51.83 Positioning Slug by 0.324ml @12:19:51.92 AS.seek 0.326ml @12:19:56.84
CSV.dial :waste @12:19:57.64 CS.seek 0ml @12:20:05.91 Flushing lower manifold's waste1 port @12:20:06.05 CSV.dial :flush @12:20:06.56 CS.seek 5ml @12:20:19.16 delay 5 seconds @12:20:24.19 CSV.dial :air @12:20:24.74 CS.seek 7ml @12:20:30.38 CSV.dial CTV @12:20:30.41
CTV.dial CSV @12:20:31.94 CBV.dial :waste1 @12:20:32.46 CS.seek 0ml @12:20:49.81 Flushing lower manifold's waste2b port @12:20:49.95 CSV.dial :flush @12:20:50.76 CS.seek 5ml @12:21:03.53 delay 5 seconds @12:21:08.56 CSV.dial :air @12:21:09.71 CS.seek 7ml @12:21:15.38 CSV.dial CTV @12:21:15.41
CTV.dial CSV @12:21:15.89 CBV.dial :waste2b @12:21:16.73 CS.seek 0ml @12:21:34.35 Disinfecting external Intake with kill @12:21:34.49 CSV.dial CSR @12:21:35.04 Pulling 8ml of CSR.kill CSR.select :kill @12:21:35.17 CS.seek 8ml @12:21:54.94 delay 5 seconds @12:22:00.68 CSR.select :air @12:22:00.82 CS.seek 10ml @12:22:06.53 CSV.dial CBV @12:22:06.56
CBV.dial CSV @12:22:07.72 CTV.dial :intake @12:22:08.53 Intake.select :open @12:22:08.65 CS.seek 2ml @12:22:29.63 Intake.select :close @12:22:29.74 CTV.dial :air @12:22:30.57 CS.reconfigure CSconfig @12:22:30.62 CS.seek 0ml @12:22:36.72 CSV.dial CSR @12:22:37.89 Pulling 5ml of CSR.flush CSR.select :flush @12:22:38.02 CS.seek 5ml @12:22:51.75 delay 5 seconds @12:22:56.78 CSR.select :air @12:22:56.91 CS.seek 10ml @12:23:10.75 CSV.dial CBV @12:23:11.86 CS.seek 0ml @12:23:38.41 Garaging FlushPuck in Collection Clamp @12:23:38.50 Elbow.reconfigure TightElbow @12:23:38.55 Elbow.seek CC @12:23:38.81 Elbow.reconfigure LooseElbow @12:23:38.91 Forearm.seek CC @12:23:39.90 Hand.close @12:23:40.55 CC.seek :open @12:23:44.95 Forearm.seek :retract @12:23:45.96 Elbow.reconfigure TightElbow @12:23:46.01 Elbow.seek :garage @12:23:49.20 Elbow.reconfigure LooseElbow @12:23:49.30 Forearm.reconfigure TightForearm @12:23:49.35 Forearm.seek :garage @12:23:50.52 Hand.open @12:23:51.23 Forearm.reconfigure LooseForearm @12:23:51.28 Forearm.seek :retract @12:23:52.32 Starting initialPurge @12:23:52.33 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:23:52.41 Hand.open @12:23:52.73 Elbow.reconfigure TightElbow @12:23:52.78 Elbow.seek FlushPuck::Garage @12:23:53.03 Elbow.reconfigure LooseElbow @12:23:53.13 Forearm.seek :garage @12:23:54.16 Hand.close @12:23:54.92 Forearm.seek :retract @12:23:55.95 Elbow.reconfigure TightElbow @12:23:56.01 Elbow.seek CC @12:23:59.02 Elbow.reconfigure LooseElbow @12:23:59.13 Forearm.reconfigure TightForearm @12:23:59.19 Forearm.seek CC @12:24:00.52 CC.seek :closed @12:24:04.77 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 1.99 @12:24:04.86 Hand.open @12:24:05.61 Forearm.reconfigure LooseForearm @12:24:05.65 Forearm.seek :retract @12:24:07.49 Flushing the Collection Valve::Manifold @12:24:07.63 CSV.dial CSR @12:24:08.80 Pulling 5ml of CSR.flush @12:24:08.81 CSR.select :flush @12:24:08.94 CS.seek 5ml @12:24:22.66 delay 5 seconds @12:24:27.69 CSR.select :air @12:24:27.87 CS.reconfigure CSfast @12:24:27.92 CS.seek 7.5ml @12:24:34.91 CSV.dial :waste @12:24:36.30 CS.reconfigure CSconfig @12:24:36.35 CS.seek 0ml @12:24:56.69 Purging external Intake @12:24:56.83 CSV.dial :flush @12:24:57.32 CS.reconfigure CSfast @12:24:57.38 CS.seek 10ml @12:25:18.00 delay 5 seconds @12:25:21.63
delay 5 seconds @12:25:23.03 ATV.dial ARV @12:25:23.79 ASV.dial :PCRlock @12:25:24.16 delay 5 seconds @12:25:26.65 CSV.dial CBV @12:25:26.68
CBV.dial CSV @12:25:27.49 CTV.dial :intake @12:25:28.33 Intake.select :open @12:25:28.45 CS.seek 0ml @12:25:29.19 Running OM Profile60C350 thermocycling @12:25:29.21 PCR.program Profile60C350 @12:25:29.32 Profile60C350 should complete in < 2:02:45 @12:25:29.33 PCR.run 2:38:26.25 @12:25:54.73
Intake.select :close @12:25:54.84 CTV.dial :air @12:25:55.66 Flushing upper manifold @12:25:55.80 CSV.dial :flush @12:25:56.61 CS.seek 5ml @12:26:09.32 delay 5 seconds @12:26:14.34 CSV.dial :air @12:26:14.89 CS.seek 7ml @12:26:20.73 CSV.dial CBV @12:26:20.77
CBV.dial CSV @12:26:21.88 CTV.dial :air @12:26:21.90 CS.seek 0ml @12:26:39.54 delay 7 seconds @12:26:46.57 CS.seek 3ml @12:26:54.60 CSV.dial :waste @12:26:55.10 CS.seek 0ml @12:27:03.19 CSV.dial :flush @12:27:03.67 CS.seek 3ml @12:27:11.76 delay 5 seconds @12:27:16.78 CSV.dial :air @12:27:17.33 CS.seek 5ml @12:27:23.26 CSV.dial :air @12:27:23.28 CS.seek 0ml @12:27:36.04 delay 7 seconds @12:27:43.07 CS.seek 3ml @12:27:51.10 CSV.dial :waste @12:27:51.91 CS.seek 0ml @12:27:59.95 Garaging FlushPuck in Collection Clamp @12:28:00.03 Elbow.reconfigure TightElbow @12:28:00.08 Elbow.seek CC @12:28:00.31 Elbow.reconfigure LooseElbow @12:28:00.42 Forearm.seek CC @12:28:01.42 Hand.close @12:28:02.17 CC.seek :open @12:28:06.55 Forearm.seek :retract @12:28:08.26 Elbow.reconfigure TightElbow @12:28:08.31 Elbow.seek :garage @12:28:11.65 Elbow.reconfigure LooseElbow @12:28:11.76 Forearm.reconfigure TightForearm @12:28:11.81 Forearm.seek :garage @12:28:12.97 Hand.open @12:28:13.74 Forearm.reconfigure LooseForearm @12:28:13.79 Forearm.seek :retract @12:28:14.83 Completed [stdphase] begun 10:01:42 @12:28:14.84 Preparing to sleep until Mon May 25 10:01:42 +0200 2020 @12:28:14.91 deferred until MFBPCR finish @12:28:14.93
Waiting for MFBPCR til 10:01CEST25-May-20 @12:28:14.94 Continuing at 10:01CEST25-May-20 @12:28:15.00 GoodNews.email "Then, in tube 3: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:150\n{1 => 22, 2 => 15, 3 => 7}\nCan@12:28:14, 9.6C, 49% humidity, 14.4psia, 12.994V, 0.834A, 0.834A avg, 10.83W",Subject: "Waiting until 10:01CEST25-May-20" @14:02:03.93 PCR.off @14:02:05.19 PCR for OM completed -- closed /var/log/esp/OM20may2412h400ml.pcr @14:02:05.26 Cleaning PCR @14:02:05.27 AFV.select :carrierSolution @14:02:05.38 delay 2 seconds @14:02:07.41 AS.reconfigure ASconfig @14:02:07.46 AS.seek 0.5ml @14:02:17.77 delay 1 second @14:02:19.45 AFV.select :holdingCoil @14:02:19.64 ASV.dial :waste1 @14:02:20.02 ATV.dial :air3 @14:02:21.26 AS.reconfigure ASslow1 @14:02:21.31 AS.seek 0.51ml @14:02:23.60 delay 2 seconds @14:02:25.62 ATV.dial :bleach @14:02:26.23 AS.seek 0.81ml @14:03:26.82 delay 3 seconds @14:03:29.85 ATV.dial :air3 @14:03:30.39 delay 3 seconds @14:03:33.48 AS.seek 0.835ml @14:03:38.79 delay 3 seconds @14:03:41.82 ATV.dial :bleach @14:03:42.43 AS.seek 0.82ml @14:03:45.67 delay 3 seconds @14:03:48.70 ARV.dial :waste @14:03:49.28 delay 3 seconds @14:03:52.31 ATV.dial :ARV @14:03:53.17 AS.reconfigure ASconfig @14:03:53.22 AS.seek 0.47ml @14:04:13.63 AFV.select :carrierSolution @14:04:13.74 delay 2 seconds @14:04:15.78 AS.seek 1ml @14:04:46.52 delay 1 second @14:04:47.54 AFV.select :holdingCoil @14:04:47.64 delay 1 second @14:04:48.67 AS.reconfigure ASfast @14:04:48.72 AS.seek 0ml @14:05:16.05 AFV.select :carrierSolution @14:05:16.16 delay 2 seconds @14:05:18.19 AS.reconfigure ASconfig @14:05:18.24 AS.seek 1ml @14:06:16.04 delay 1 second @14:06:17.06 AFV.select :holdingCoil @14:06:17.16 delay 1 second @14:06:18.19 AS.reconfigure ASfast @14:06:18.24 AS.seek 0ml @14:06:45.58 AFV.select :carrierSolution @14:06:45.69 delay 2 seconds @14:06:47.72 AS.reconfigure ASconfig @14:06:47.77 AS.seek 1ml @14:07:45.52 delay 1 second @14:07:46.54 AFV.select :holdingCoil @14:07:46.64 delay 1 second @14:07:47.67 AS.reconfigure ASfast @14:07:47.72 AS.seek 0ml @14:08:15.05 ARV.dial between carrier and air1 @14:08:15.52 ATV.dial :air3 @14:08:16.75 AS.reconfigure ASslow1 @14:08:16.80 AS.seek 0.01ml @14:08:19.10 delay 2 seconds @14:08:21.12 ATV.dial :bleach @14:08:21.73 AS.seek 0.81ml @14:11:02.88 delay 3 seconds @14:11:05.91 ATV.dial :air3 @14:11:06.45 delay 3 seconds @14:11:09.54 AS.seek 0.835ml @14:11:14.86 delay 3 seconds @14:11:17.89 ATV.dial :bleach @14:11:18.50 AS.seek 0.82ml @14:11:21.76 delay 3 seconds @14:11:24.79 ATV.dial PCR @14:11:25.16 AS.reconfigure ASconfig @14:11:25.21 AS.seek :empty @14:12:12.68 PCR.program :bleachProfile @14:12:12.74 PCR program should complete in < 2:45 @14:12:12.75 PCR.run 10:00 @14:15:26.71 PCR.off @14:15:26.74 AFV.select :carrierSolution @14:15:26.85 delay 2 seconds @14:15:28.89 AS.seek 1ml @14:16:26.78 delay 1 second @14:16:27.80 AFV.select :holdingCoil @14:16:27.90 delay 1 second @14:16:28.93 AS.reconfigure ASfast @14:16:28.98 AS.seek 0ml @14:16:56.31 delay 3 seconds @14:16:59.34 AFV.select :carrierSolution @14:16:59.44 delay 2 seconds @14:17:01.47 AS.reconfigure ASconfig @14:17:01.52 AS.seek 1ml @14:17:59.26 delay 1 second @14:18:00.28 AFV.select :holdingCoil @14:18:00.38 delay 1 second @14:18:01.41 AS.reconfigure ASfast @14:18:01.46 AS.seek 0ml @14:18:29.54 delay 3 seconds @14:18:32.57 AFV.select :carrierSolution @14:18:32.67 delay 2 seconds @14:18:34.70 AS.reconfigure ASconfig @14:18:34.76 AS.seek 1ml @14:19:32.50 delay 1 second @14:19:33.52 AFV.select :holdingCoil @14:19:33.62 delay 1 second @14:19:34.65 AS.reconfigure ASfast @14:19:34.70 AS.seek 0ml @14:20:02.03 delay 3 seconds @14:20:05.06 AFV.select :carrierSolution @14:20:05.16 delay 2 seconds @14:20:07.20 AS.reconfigure ASconfig @14:20:07.30 AS.seek 1ml @14:21:05.05 delay 1 second @14:21:06.07 AFV.select :holdingCoil @14:21:06.17 delay 1 second @14:21:07.20 AS.reconfigure ASfast @14:21:07.25 AS.seek 0ml @14:21:34.61 delay 3 seconds @14:21:37.64 AFV.select :carrierSolution @14:21:37.74 delay 2 seconds @14:21:39.77 AS.reconfigure ASconfig @14:21:39.83 AS.seek 1ml @14:22:37.61 delay 1 second @14:22:38.63 AFV.select :holdingCoil @14:22:38.73 delay 1 second @14:22:39.76 AS.reconfigure ASnorm @14:22:39.81 AS.seek 0ml @14:23:30.54 Setting up PCR for ML -- writing /var/log/esp/ML20may2414h400ml.pcr @14:23:30.56 Building PCR Slug from ASV with MM2 mix, PP4 primer @14:23:30.58 Priming with carrier @14:23:30.59 ATV.dial ARV @14:23:31.19 ARV.dial :carrier @14:23:31.56 AS.seek 0.5ml @14:23:57.19 AFV.select :carrierSolution @14:23:57.30 delay 2 seconds @14:23:59.33 AS.reconfigure ASconfig @14:23:59.38 AS.seek 1ml @14:24:28.44 delay 1 second @14:24:29.46 AFV.select :holdingCoil @14:24:29.59 delay 1 second @14:24:30.62 ATV.dial :waste @14:24:32.19 AS.reconfigure ASnorm @14:24:32.25 AS.seek :empty @14:25:22.99 AFV.select :carrierSolution @14:25:23.10 delay 2 seconds @14:25:25.13 AS.reconfigure ASconfig @14:25:25.18 AS.seek 0.5ml @14:25:54.25 delay 1 second @14:25:55.27 AFV.select :holdingCoil @14:25:55.37 Building Slug @14:25:55.38 ATV.dial :ARV @14:25:57.03 delay 2 seconds @14:25:59.06 ARV.dial :air1 @14:25:59.44 delay 2 seconds @14:26:01.53 AS.reconfigure ASslow2 @14:26:01.58 AS.seek 0.506ml @14:26:07.83 delay 2 seconds @14:26:09.86 ARV.dial :MM2 @14:26:11.27 delay 2 seconds @14:26:13.30 Adding MM2 @14:26:13.39 AS.seek 0.524ml @14:26:31.66 delay 25 seconds @14:26:56.69 ARV.dial :PP4 @14:26:57.07 Adding PP4 @14:26:57.09 delay 2 seconds @14:26:59.17 AS.seek 0.53ml @14:27:05.57 delay 10 seconds @14:27:15.60 ARV.dial :air3 @14:27:15.99 adding ARV air @14:27:16.01 delay 2 seconds @14:27:18.09 AS.seek 0.536ml @14:27:24.39 delay 5 seconds @14:27:29.42 ARV.dial :carrier @14:27:30.61 delay 7 seconds @14:27:37.71 AS.seek 0.594ml @14:28:36.19 delay 5 seconds @14:28:41.22 ATV.dial :ASV @14:28:41.61 delay 2 seconds @14:28:43.70 AS.seek 0.6ml @14:28:49.86 delay 4 seconds @14:28:53.89 ATV.dial ARV @14:28:54.52 delay 2 seconds @14:28:56.55 ARV.dial :carrier @14:28:56.56 delay 2 seconds @14:28:58.65 AS.seek 0.65ml @14:29:49.30 delay 4 seconds @14:29:53.33 ATV.dial PCR @14:29:53.91 ASV.dial :waste1 @14:29:53.92 ARV.dial :air1 @14:29:54.31 Positioning Slug by 0.324ml @14:29:54.40 AS.seek 0.326ml @14:35:20.48 delay 5 seconds @14:35:25.51 ATV.dial ARV @14:35:26.24 ASV.dial :PCRlock @14:35:26.62 delay 5 seconds @14:35:31.65 Running ML Profile60C350 thermocycling @14:35:31.67 PCR.program Profile60C350 @14:35:32.52 Profile60C350 should complete in < 2:02:45 @14:35:32.53 PCR.run 2:38:26.25 @16:11:49.02 PCR.off @16:11:50.28 PCR for ML completed -- closed /var/log/esp/ML20may2414h400ml.pcr @16:11:50.35 Cleaning PCR @16:11:50.36 AFV.select :carrierSolution @16:11:50.47 delay 2 seconds @16:11:52.50 AS.reconfigure ASconfig @16:11:52.55 AS.seek 0.5ml @16:12:02.83 delay 1 second @16:12:03.85 AFV.select :holdingCoil @16:12:03.95 ASV.dial :waste1 @16:12:04.33 ATV.dial :air3 @16:12:05.57 AS.reconfigure ASslow1 @16:12:05.62 AS.seek 0.51ml @16:12:07.92 delay 2 seconds @16:12:09.94 ATV.dial :bleach @16:12:10.54 AS.seek 0.81ml @16:13:11.16 delay 3 seconds @16:13:14.19 ATV.dial :air3 @16:13:14.72 delay 3 seconds @16:13:17.81 AS.seek 0.835ml @16:13:23.11 delay 3 seconds @16:13:26.14 ATV.dial :bleach @16:13:26.74 AS.seek 0.82ml @16:13:30.00 delay 3 seconds @16:13:33.03 ARV.dial :waste @16:13:33.63 delay 3 seconds @16:13:36.66 ATV.dial :ARV @16:13:37.50 AS.reconfigure ASconfig @16:13:37.56 AS.seek 0.47ml @16:13:57.97 AFV.select :carrierSolution @16:13:58.08 delay 2 seconds @16:14:00.12 AS.seek 1ml @16:14:30.86 delay 1 second @16:14:32.63 AFV.select :holdingCoil @16:14:32.73 delay 1 second @16:14:33.76 AS.reconfigure ASfast @16:14:33.81 AS.seek 0ml @16:15:01.13 AFV.select :carrierSolution @16:15:01.24 delay 2 seconds @16:15:03.27 AS.reconfigure ASconfig @16:15:03.32 AS.seek 1ml @16:16:01.10 delay 1 second @16:16:02.12 AFV.select :holdingCoil @16:16:02.22 delay 1 second @16:16:03.25 AS.reconfigure ASfast @16:16:03.30 AS.seek 0ml @16:16:30.63 AFV.select :carrierSolution @16:16:30.74 delay 2 seconds @16:16:32.77 AS.reconfigure ASconfig @16:16:32.82 AS.seek 1ml @16:17:30.66 delay 1 second @16:17:31.68 AFV.select :holdingCoil @16:17:31.78 delay 1 second @16:17:32.81 AS.reconfigure ASfast @16:17:32.86 AS.seek 0ml @16:18:00.24 ARV.dial between carrier and air1 @16:18:00.74 ATV.dial :air3 @16:18:01.97 AS.reconfigure ASslow1 @16:18:02.02 AS.seek 0.01ml @16:18:04.35 delay 2 seconds @16:18:06.37 ATV.dial :bleach @16:18:06.98 AS.seek 0.81ml @16:20:48.13 delay 3 seconds @16:20:51.16 ATV.dial :air3 @16:20:51.69 delay 3 seconds @16:20:54.78 AS.seek 0.835ml @16:21:00.08 delay 3 seconds @16:21:03.11 ATV.dial :bleach @16:21:03.72 AS.seek 0.82ml @16:21:07.02 delay 3 seconds @16:21:10.05 ATV.dial PCR @16:21:10.44 AS.reconfigure ASconfig @16:21:10.49 AS.seek :empty @16:21:57.95 PCR.program :bleachProfile @16:21:58.00 PCR program should complete in < 2:45 @16:21:58.02 PCR.run 10:00 @16:25:11.96 PCR.off @16:25:11.98 AFV.select :carrierSolution @16:25:12.09 delay 2 seconds @16:25:14.13 AS.seek 1ml @16:26:11.98 delay 1 second @16:26:13.00 AFV.select :holdingCoil @16:26:13.10 delay 1 second @16:26:14.13 AS.reconfigure ASfast @16:26:14.18 AS.seek 0ml @16:26:41.51 delay 3 seconds @16:26:44.54 AFV.select :carrierSolution @16:26:44.64 delay 2 seconds @16:26:46.67 AS.reconfigure ASconfig @16:26:46.73 AS.seek 1ml @16:27:44.48 delay 1 second @16:27:45.50 AFV.select :holdingCoil @16:27:45.60 delay 1 second @16:27:46.63 AS.reconfigure ASfast @16:27:46.68 AS.seek 0ml @16:28:14.03 delay 3 seconds @16:28:17.06 AFV.select :carrierSolution @16:28:17.16 delay 2 seconds @16:28:19.19 AS.reconfigure ASconfig @16:28:19.25 AS.seek 1ml @16:29:17.03 delay 1 second @16:29:18.05 AFV.select :holdingCoil @16:29:18.15 delay 1 second @16:29:19.18 AS.reconfigure ASfast @16:29:19.23 AS.seek 0ml @16:29:46.58 delay 3 seconds @16:29:49.61 AFV.select :carrierSolution @16:29:49.71 delay 2 seconds @16:29:51.74 AS.reconfigure ASconfig @16:29:51.83 AS.seek 1ml @16:30:49.59 delay 1 second @16:30:50.61 AFV.select :holdingCoil @16:30:50.71 delay 1 second @16:30:51.74 AS.reconfigure ASfast @16:30:51.79 AS.seek 0ml @16:31:19.12 delay 3 seconds @16:31:22.15 AFV.select :carrierSolution @16:31:22.25 delay 2 seconds @16:31:24.28 AS.reconfigure ASconfig @16:31:24.34 AS.seek 1ml @16:32:22.09 delay 1 second @16:32:23.11 AFV.select :holdingCoil @16:32:23.21 delay 1 second @16:32:24.24 AS.reconfigure ASnorm @16:32:24.29 AS.seek 0ml @16:33:15.00 Setting up PCR for CG -- writing /var/log/esp/CG20may2416h400ml.pcr @16:33:15.02 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:33:15.03 Priming with carrier @16:33:15.04 ATV.dial ARV @16:33:15.63 ARV.dial :carrier @16:33:15.99 AS.seek 0.5ml @16:33:41.52 AFV.select :carrierSolution @16:33:42.37 delay 2 seconds @16:33:44.40 AS.reconfigure ASconfig @16:33:44.45 AS.seek 1ml @16:34:13.47 delay 1 second @16:34:14.49 AFV.select :holdingCoil @16:34:14.59 delay 1 second @16:34:15.62 ATV.dial :waste @16:34:17.19 AS.reconfigure ASnorm @16:34:17.25 AS.seek :empty @16:35:08.00 AFV.select :carrierSolution @16:35:08.11 delay 2 seconds @16:35:10.14 AS.reconfigure ASconfig @16:35:10.19 AS.seek 0.5ml @16:35:39.27 delay 1 second @16:35:40.29 AFV.select :holdingCoil @16:35:40.39 Building Slug @16:35:40.40 ATV.dial :ARV @16:35:41.96 delay 2 seconds @16:35:43.99 ARV.dial :air1 @16:35:44.39 delay 2 seconds @16:35:46.48 AS.reconfigure ASslow2 @16:35:46.54 AS.seek 0.506ml @16:35:52.94 delay 2 seconds @16:35:54.96 ARV.dial :MM1 @16:35:55.55 delay 2 seconds @16:35:57.58 Adding MM1 @16:35:57.65 AS.seek 0.524ml @16:36:15.99 delay 25 seconds @16:36:41.02 ARV.dial :PP2 @16:36:41.41 Adding PP2 @16:36:41.43 delay 2 seconds @16:36:43.51 AS.seek 0.53ml @16:36:49.92 delay 10 seconds @16:36:59.95 ARV.dial :air2 @16:37:00.33 adding ARV air @16:37:00.35 delay 2 seconds @16:37:02.43 AS.seek 0.536ml @16:37:08.64 delay 5 seconds @16:37:13.67 ARV.dial :carrier @16:37:14.88 delay 7 seconds @16:37:21.97 AS.seek 0.594ml @16:38:20.63 delay 5 seconds @16:38:25.66 ATV.dial :ASV @16:38:26.05 delay 2 seconds @16:38:28.14 AS.seek 0.6ml @16:38:34.47 delay 4 seconds @16:38:38.50 ATV.dial ARV @16:38:39.04 delay 2 seconds @16:38:41.07 ARV.dial :carrier @16:38:41.08 delay 2 seconds @16:38:43.17 AS.seek 0.65ml @16:39:33.58 delay 4 seconds @16:39:37.61 ATV.dial PCR @16:39:38.19 ASV.dial :waste1 @16:39:38.20 ARV.dial :air1 @16:39:38.59 Positioning Slug by 0.324ml @16:39:38.68 AS.seek 0.326ml @16:45:04.70 delay 5 seconds @16:45:09.73 ATV.dial ARV @16:45:10.46 ASV.dial :PCRlock @16:45:10.85 delay 5 seconds @16:45:15.88 Running CG Profile60C300 thermocycling @16:45:15.90 PCR.program Profile60C300 @16:45:16.00 Profile60C300 should complete in < 1:59:25 @16:45:16.01 PCR.run 2:34:16.25 @18:20:20.96 PCR.off @18:20:21.63 PCR for CG completed -- closed /var/log/esp/CG20may2416h400ml.pcr @18:20:21.70 Cleaning PCR @18:20:21.71 AFV.select :carrierSolution @18:20:21.82 delay 2 seconds @18:20:23.85 AS.reconfigure ASconfig @18:20:23.90 AS.seek 0.5ml @18:20:34.22 delay 1 second @18:20:35.24 AFV.select :holdingCoil @18:20:35.34 ASV.dial :waste1 @18:20:35.73 ATV.dial :air3 @18:20:36.96 AS.reconfigure ASslow1 @18:20:37.01 AS.seek 0.51ml @18:20:39.23 delay 2 seconds @18:20:41.25 ATV.dial :bleach @18:20:41.85 AS.seek 0.81ml @18:21:42.45 delay 3 seconds @18:21:45.48 ATV.dial :air3 @18:21:46.01 delay 3 seconds @18:21:49.10 AS.seek 0.835ml @18:21:54.45 delay 3 seconds @18:21:57.48 ATV.dial :bleach @18:21:58.08 AS.seek 0.82ml @18:22:01.40 delay 3 seconds @18:22:04.43 ARV.dial :waste @18:22:05.03 delay 3 seconds @18:22:08.06 ATV.dial :ARV @18:22:08.90 AS.reconfigure ASconfig @18:22:08.96 AS.seek 0.47ml @18:22:29.37 AFV.select :carrierSolution @18:22:29.48 delay 2 seconds @18:22:31.52 AS.seek 1ml @18:23:02.31 delay 1 second @18:23:03.33 AFV.select :holdingCoil @18:23:03.43 delay 1 second @18:23:04.46 AS.reconfigure ASfast @18:23:04.51 AS.seek 0ml @18:23:31.84 AFV.select :carrierSolution @18:23:31.95 delay 2 seconds @18:23:33.98 AS.reconfigure ASconfig @18:23:34.03 AS.seek 1ml @18:24:31.80 delay 1 second @18:24:32.82 AFV.select :holdingCoil @18:24:32.92 delay 1 second @18:24:33.95 AS.reconfigure ASfast @18:24:34.00 AS.seek 0ml @18:25:01.34 AFV.select :carrierSolution @18:25:01.45 delay 2 seconds @18:25:03.48 AS.reconfigure ASconfig @18:25:03.53 AS.seek 1ml @18:26:01.30 delay 1 second @18:26:02.32 AFV.select :holdingCoil @18:26:02.42 delay 1 second @18:26:03.45 AS.reconfigure ASfast @18:26:03.50 AS.seek 0ml @18:26:30.85 ARV.dial between carrier and air1 @18:26:31.35 ATV.dial :air3 @18:26:32.57 AS.reconfigure ASslow1 @18:26:32.62 AS.seek 0.01ml @18:26:34.91 delay 2 seconds @18:26:36.93 ATV.dial :bleach @18:26:37.54 AS.seek 0.81ml @18:29:18.68 delay 3 seconds @18:29:21.71 ATV.dial :air3 @18:29:22.25 delay 3 seconds @18:29:25.34 AS.seek 0.835ml @18:29:30.66 delay 3 seconds @18:29:33.69 ATV.dial :bleach @18:29:34.29 AS.seek 0.82ml @18:29:37.54 delay 3 seconds @18:29:40.56 ATV.dial PCR @18:29:40.96 AS.reconfigure ASconfig @18:29:41.01 AS.seek :empty @18:30:28.47 PCR.program :bleachProfile @18:30:28.53 PCR program should complete in < 2:45 @18:30:28.54 PCR.run 10:00 @18:33:43.22 PCR.off @18:33:43.24 AFV.select :carrierSolution @18:33:43.35 delay 2 seconds @18:33:45.39 AS.seek 1ml @18:34:43.19 delay 1 second @18:34:44.21 AFV.select :holdingCoil @18:34:44.31 delay 1 second @18:34:45.34 AS.reconfigure ASfast @18:34:45.39 AS.seek 0ml @18:35:12.71 delay 3 seconds @18:35:15.74 AFV.select :carrierSolution @18:35:15.84 delay 2 seconds @18:35:17.87 AS.reconfigure ASconfig @18:35:17.92 AS.seek 1ml @18:36:15.71 delay 1 second @18:36:16.73 AFV.select :holdingCoil @18:36:16.83 delay 1 second @18:36:17.86 AS.reconfigure ASfast @18:36:17.91 AS.seek 0ml @18:36:45.24 delay 3 seconds @18:36:48.27 AFV.select :carrierSolution @18:36:48.37 delay 2 seconds @18:36:50.41 AS.reconfigure ASconfig @18:36:50.46 AS.seek 1ml @18:37:48.24 delay 1 second @18:37:49.26 AFV.select :holdingCoil @18:37:49.36 delay 1 second @18:37:50.39 AS.reconfigure ASfast @18:37:50.44 AS.seek 0ml @18:38:17.78 delay 3 seconds @18:38:20.81 AFV.select :carrierSolution @18:38:20.91 delay 2 seconds @18:38:22.94 AS.reconfigure ASconfig @18:38:22.99 AS.seek 1ml @18:39:20.74 delay 1 second @18:39:21.76 AFV.select :holdingCoil @18:39:21.86 delay 1 second @18:39:22.89 AS.reconfigure ASfast @18:39:22.94 AS.seek 0ml @18:39:50.26 delay 3 seconds @18:39:53.29 AFV.select :carrierSolution @18:39:53.39 delay 2 seconds @18:39:55.42 AS.reconfigure ASconfig @18:39:55.47 AS.seek 1ml @18:40:53.21 delay 1 second @18:40:54.23 AFV.select :holdingCoil @18:40:54.33 delay 1 second @18:40:55.36 AS.reconfigure ASnorm @18:40:55.41 AS.seek 0ml @18:41:46.14 Setting up PCR for NM -- writing /var/log/esp/NM20may2418h400ml.pcr @18:41:46.16 Building PCR Slug from ASV with MM1 mix, PP1 primer @18:41:46.17 Priming with carrier @18:41:46.18 ATV.dial ARV @18:41:46.76 ARV.dial :carrier @18:41:47.11 AS.seek 0.5ml @18:42:12.72 AFV.select :carrierSolution @18:42:12.83 delay 2 seconds @18:42:14.86 AS.reconfigure ASconfig @18:42:14.91 AS.seek 1ml @18:42:43.92 delay 1 second @18:42:44.94 AFV.select :holdingCoil @18:42:45.04 delay 1 second @18:42:46.07 ATV.dial :waste @18:42:47.64 AS.reconfigure ASnorm @18:42:47.70 AS.seek :empty @18:43:38.44 AFV.select :carrierSolution @18:43:38.55 delay 2 seconds @18:43:40.58 AS.reconfigure ASconfig @18:43:40.63 AS.seek 0.5ml @18:44:09.69 delay 1 second @18:44:10.71 AFV.select :holdingCoil @18:44:10.81 Building Slug @18:44:10.82 ATV.dial :ARV @18:44:12.37 delay 2 seconds @18:44:14.40 ARV.dial :air1 @18:44:14.79 delay 2 seconds @18:44:16.87 AS.reconfigure ASslow2 @18:44:16.93 AS.seek 0.506ml @18:44:23.20 delay 2 seconds @18:44:25.22 ARV.dial :MM1 @18:44:25.81 delay 2 seconds @18:44:27.84 Adding MM1 @18:44:27.91 AS.seek 0.524ml @18:44:46.28 delay 25 seconds @18:45:11.31 ARV.dial :PP1 @18:45:11.69 Adding PP1 @18:45:11.71 delay 2 seconds @18:45:13.79 AS.seek 0.53ml @18:45:19.97 delay 10 seconds @18:45:30.00 ARV.dial :air1 @18:45:30.37 adding ARV air @18:45:30.39 delay 2 seconds @18:45:32.47 AS.seek 0.536ml @18:45:38.70 delay 5 seconds @18:45:43.73 ARV.dial :carrier @18:45:44.12 delay 7 seconds @18:45:51.21 AS.seek 0.594ml @18:46:50.59 delay 5 seconds @18:46:55.62 ATV.dial :ASV @18:46:56.02 delay 2 seconds @18:46:58.11 AS.seek 0.6ml @18:47:04.41 delay 4 seconds @18:47:08.44 ATV.dial ARV @18:47:08.98 delay 2 seconds @18:47:11.01 ARV.dial :carrier @18:47:11.02 delay 2 seconds @18:47:13.11 AS.seek 0.65ml @18:48:03.61 delay 4 seconds @18:48:07.64 ATV.dial PCR @18:48:08.22 ASV.dial :waste1 @18:48:08.23 ARV.dial :air1 @18:48:08.64 Positioning Slug by 0.324ml @18:48:08.73 AS.seek 0.326ml @18:53:34.74 delay 5 seconds @18:53:39.77 ATV.dial ARV @18:53:40.51 ASV.dial :PCRlock @18:53:40.90 delay 5 seconds @18:53:45.93 Running NM Profile60C300 thermocycling @18:53:45.94 PCR.program Profile60C300 @18:53:46.04 Profile60C300 should complete in < 1:59:25 @18:53:46.05 PCR.run 2:34:16.25 @20:28:37.25 PCR.off @20:28:37.91 PCR for NM completed -- closed /var/log/esp/NM20may2418h400ml.pcr @20:28:37.98 Cleaning PCR @20:28:37.99 AFV.select :carrierSolution @20:28:38.10 delay 2 seconds @20:28:40.13 AS.reconfigure ASconfig @20:28:40.18 AS.seek 0.5ml @20:28:50.48 delay 1 second @20:28:51.50 AFV.select :holdingCoil @20:28:51.60 ASV.dial :waste1 @20:28:51.98 ATV.dial :air3 @20:28:53.19 AS.reconfigure ASslow1 @20:28:53.25 AS.seek 0.51ml @20:28:55.55 delay 2 seconds @20:28:57.57 ATV.dial :bleach @20:28:58.17 AS.seek 0.81ml @20:29:58.79 delay 3 seconds @20:30:01.81 ATV.dial :air3 @20:30:02.35 delay 3 seconds @20:30:05.44 AS.seek 0.835ml @20:30:10.73 delay 3 seconds @20:30:13.76 ATV.dial :bleach @20:30:15.12 AS.seek 0.82ml @20:30:18.38 delay 3 seconds @20:30:21.41 ARV.dial :waste @20:30:22.01 delay 3 seconds @20:30:25.04 ATV.dial :ARV @20:30:26.00 AS.reconfigure ASconfig @20:30:26.05 AS.seek 0.47ml @20:30:46.46 AFV.select :carrierSolution @20:30:46.57 delay 2 seconds @20:30:48.61 AS.seek 1ml @20:31:19.37 delay 1 second @20:31:20.39 AFV.select :holdingCoil @20:31:20.49 delay 1 second @20:31:21.52 AS.reconfigure ASfast @20:31:21.57 AS.seek 0ml @20:31:48.92 AFV.select :carrierSolution @20:31:49.03 delay 2 seconds @20:31:51.06 AS.reconfigure ASconfig @20:31:51.11 AS.seek 1ml @20:32:48.85 delay 1 second @20:32:49.87 AFV.select :holdingCoil @20:32:49.97 delay 1 second @20:32:51.00 AS.reconfigure ASfast @20:32:51.05 AS.seek 0ml @20:33:18.35 AFV.select :carrierSolution @20:33:18.46 delay 2 seconds @20:33:20.49 AS.reconfigure ASconfig @20:33:20.54 AS.seek 1ml @20:34:18.31 delay 1 second @20:34:19.33 AFV.select :holdingCoil @20:34:19.43 delay 1 second @20:34:20.46 AS.reconfigure ASfast @20:34:20.51 AS.seek 0ml @20:34:47.82 ARV.dial between carrier and air1 @20:34:48.31 ATV.dial :air3 @20:34:49.53 AS.reconfigure ASslow1 @20:34:49.58 AS.seek 0.01ml @20:34:51.87 delay 2 seconds @20:34:53.89 ATV.dial :bleach @20:34:54.50 AS.seek 0.81ml @20:37:35.62 delay 3 seconds @20:37:38.65 ATV.dial :air3 @20:37:39.19 delay 3 seconds @20:37:42.28 AS.seek 0.835ml @20:37:47.59 delay 3 seconds @20:37:50.62 ATV.dial :bleach @20:37:51.22 AS.seek 0.82ml @20:37:54.53 delay 3 seconds @20:37:57.56 ATV.dial PCR @20:37:57.95 AS.reconfigure ASconfig @20:37:58.00 AS.seek :empty @20:38:45.47 PCR.program :bleachProfile @20:38:45.53 PCR program should complete in < 2:45 @20:38:45.54 PCR.run 10:00 @20:41:59.48 PCR.off @20:41:59.51 AFV.select :carrierSolution @20:41:59.62 delay 2 seconds @20:42:01.66 AS.seek 1ml @20:42:59.47 delay 1 second @20:43:00.49 AFV.select :holdingCoil @20:43:00.59 delay 1 second @20:43:01.62 AS.reconfigure ASfast @20:43:01.67 AS.seek 0ml @20:43:29.02 delay 3 seconds @20:43:32.05 AFV.select :carrierSolution @20:43:32.15 delay 2 seconds @20:43:34.18 AS.reconfigure ASconfig @20:43:34.24 AS.seek 1ml @20:44:32.00 delay 1 second @20:44:33.02 AFV.select :holdingCoil @20:44:33.12 delay 1 second @20:44:34.15 AS.reconfigure ASfast @20:44:34.20 AS.seek 0ml @20:45:01.53 delay 3 seconds @20:45:04.56 AFV.select :carrierSolution @20:45:04.66 delay 2 seconds @20:45:06.69 AS.reconfigure ASconfig @20:45:06.74 AS.seek 1ml @20:46:04.52 delay 1 second @20:46:05.54 AFV.select :holdingCoil @20:46:05.64 delay 1 second @20:46:06.67 AS.reconfigure ASfast @20:46:06.72 AS.seek 0ml @20:46:34.07 delay 3 seconds @20:46:37.10 AFV.select :carrierSolution @20:46:37.20 delay 2 seconds @20:46:39.23 AS.reconfigure ASconfig @20:46:39.29 AS.seek 1ml @20:47:37.02 delay 1 second @20:47:38.04 AFV.select :holdingCoil @20:47:38.14 delay 1 second @20:47:39.17 AS.reconfigure ASfast @20:47:39.22 AS.seek 0ml @20:48:06.55 delay 3 seconds @20:48:09.58 AFV.select :carrierSolution @20:48:09.68 delay 2 seconds @20:48:11.71 AS.reconfigure ASconfig @20:48:11.77 AS.seek 1ml @20:49:09.51 delay 1 second @20:49:11.27 AFV.select :holdingCoil @20:49:11.37 delay 1 second @20:49:12.40 AS.reconfigure ASnorm @20:49:12.45 AS.seek 0ml @20:50:03.18 Setting up PCR for IPc -- writing /var/log/esp/IPc20may2420h400ml.pcr @20:50:03.20 Building PCR Slug from ASV with MM3 mix, PP6 primer @20:50:03.21 Priming with carrier @20:50:03.22 ATV.dial ARV @20:50:03.80 ARV.dial :carrier @20:50:04.18 AS.seek 0.5ml @20:50:29.77 AFV.select :carrierSolution @20:50:29.88 delay 2 seconds @20:50:31.91 AS.reconfigure ASconfig @20:50:31.96 AS.seek 1ml @20:51:00.98 delay 1 second @20:51:02.00 AFV.select :holdingCoil @20:51:02.10 delay 1 second @20:51:03.13 ATV.dial :waste @20:51:04.69 AS.reconfigure ASnorm @20:51:04.75 AS.seek :empty @20:51:55.51 AFV.select :carrierSolution @20:51:55.62 delay 2 seconds @20:51:57.65 AS.reconfigure ASconfig @20:51:57.70 AS.seek 0.5ml @20:52:26.77 delay 1 second @20:52:27.79 AFV.select :holdingCoil @20:52:27.89 Building Slug @20:52:27.90 ATV.dial :ARV @20:52:29.46 delay 2 seconds @20:52:31.49 ARV.dial :air1 @20:52:31.88 delay 2 seconds @20:52:33.97 AS.reconfigure ASslow2 @20:52:34.02 AS.seek 0.506ml @20:52:40.23 delay 2 seconds @20:52:42.25 ARV.dial :MM3 @20:52:43.26 delay 2 seconds @20:52:45.29 Adding MM3 @20:52:45.37 AS.seek 0.524ml @20:53:03.70 delay 25 seconds @20:53:28.73 ARV.dial :PP6 @20:53:29.12 Adding PP6 @20:53:29.14 delay 2 seconds @20:53:31.22 AS.seek 0.53ml @20:53:37.41 delay 10 seconds @20:53:47.44 ARV.dial :air1 @20:53:48.23 adding ARV air @20:53:48.25 delay 2 seconds @20:53:50.33 AS.seek 0.536ml @20:53:56.62 delay 5 seconds @20:54:01.65 ARV.dial :carrier @20:54:02.06 delay 7 seconds @20:54:09.15 AS.seek 0.594ml @20:55:08.01 delay 5 seconds @20:55:13.04 ATV.dial :ASV @20:55:13.43 delay 2 seconds @20:55:15.52 AS.seek 0.6ml @20:55:21.82 delay 4 seconds @20:55:25.85 ATV.dial ARV @20:55:26.37 delay 2 seconds @20:55:28.40 ARV.dial :carrier @20:55:28.41 delay 2 seconds @20:55:30.50 AS.seek 0.65ml @20:56:21.10 delay 4 seconds @20:56:25.13 ATV.dial PCR @20:56:25.71 ASV.dial :waste1 @20:56:25.72 ARV.dial :air1 @20:56:26.11 Positioning Slug by 0.324ml @20:56:26.20 AS.seek 0.326ml @21:01:52.23 delay 5 seconds @21:01:57.26 ATV.dial ARV @21:01:58.00 ASV.dial :PCRlock @21:01:58.39 delay 5 seconds @21:02:03.42 Running IPc ProfileIPc thermocycling @21:02:03.43 PCR.program ProfileIPc @21:02:03.54 ProfileIPc should complete in < 1:36:15 @21:02:03.55 PCR.run 2:05:18.75 @22:20:15.97 PCR.off @22:20:16.52 PCR for IPc completed -- closed /var/log/esp/IPc20may2420h400ml.pcr @22:20:16.59 Cleaning PCR @22:20:16.60 AFV.select :carrierSolution @22:20:16.71 delay 2 seconds @22:20:18.74 AS.reconfigure ASconfig @22:20:18.79 AS.seek 0.5ml @22:20:29.10 delay 1 second @22:20:30.12 AFV.select :holdingCoil @22:20:30.22 ASV.dial :waste1 @22:20:30.60 ATV.dial :air3 @22:20:31.83 AS.reconfigure ASslow1 @22:20:31.89 AS.seek 0.51ml @22:20:34.09 delay 2 seconds @22:20:36.12 ATV.dial :bleach @22:20:36.73 AS.seek 0.81ml @22:21:37.33 delay 3 seconds @22:21:40.37 ATV.dial :air3 @22:21:40.90 delay 3 seconds @22:21:43.99 AS.seek 0.835ml @22:21:49.28 delay 3 seconds @22:21:52.31 ATV.dial :bleach @22:21:52.92 AS.seek 0.82ml @22:21:56.23 delay 3 seconds @22:21:59.26 ARV.dial :waste @22:21:59.84 delay 3 seconds @22:22:02.87 ATV.dial :ARV @22:22:03.72 AS.reconfigure ASconfig @22:22:03.78 AS.seek 0.47ml @22:22:24.20 AFV.select :carrierSolution @22:22:24.31 delay 2 seconds @22:22:26.35 AS.seek 1ml @22:22:57.12 delay 1 second @22:22:58.14 AFV.select :holdingCoil @22:22:58.24 delay 1 second @22:22:59.27 AS.reconfigure ASfast @22:22:59.32 AS.seek 0ml @22:23:26.67 AFV.select :carrierSolution @22:23:26.78 delay 2 seconds @22:23:28.81 AS.reconfigure ASconfig @22:23:28.86 AS.seek 1ml @22:24:26.63 delay 1 second @22:24:27.65 AFV.select :holdingCoil @22:24:27.75 delay 1 second @22:24:28.78 AS.reconfigure ASfast @22:24:28.83 AS.seek 0ml @22:24:56.18 AFV.select :carrierSolution @22:24:56.29 delay 2 seconds @22:24:58.32 AS.reconfigure ASconfig @22:24:58.37 AS.seek 1ml @22:25:56.15 delay 1 second @22:25:57.17 AFV.select :holdingCoil @22:25:57.27 delay 1 second @22:25:58.30 AS.reconfigure ASfast @22:25:58.35 AS.seek 0ml @22:26:25.68 ARV.dial between carrier and air1 @22:26:26.17 ATV.dial :air3 @22:26:27.41 AS.reconfigure ASslow1 @22:26:27.46 AS.seek 0.01ml @22:26:29.76 delay 2 seconds @22:26:31.78 ATV.dial :bleach @22:26:32.39 AS.seek 0.81ml @22:29:13.53 delay 3 seconds @22:29:16.56 ATV.dial :air3 @22:29:17.09 delay 3 seconds @22:29:20.18 AS.seek 0.835ml @22:29:25.45 delay 3 seconds @22:29:28.48 ATV.dial :bleach @22:29:29.08 AS.seek 0.82ml @22:29:32.37 delay 3 seconds @22:29:35.40 ATV.dial PCR @22:29:35.80 AS.reconfigure ASconfig @22:29:35.85 AS.seek :empty @22:30:23.33 PCR.program :bleachProfile @22:30:23.38 PCR program should complete in < 2:45 @22:30:23.39 PCR.run 10:00 @22:33:37.33 PCR.off @22:33:37.35 AFV.select :carrierSolution @22:33:37.46 delay 2 seconds @22:33:39.50 AS.seek 1ml @22:34:37.36 delay 1 second @22:34:38.38 AFV.select :holdingCoil @22:34:38.48 delay 1 second @22:34:39.51 AS.reconfigure ASfast @22:34:39.56 AS.seek 0ml @22:35:06.89 delay 3 seconds @22:35:09.92 AFV.select :carrierSolution @22:35:10.02 delay 2 seconds @22:35:12.05 AS.reconfigure ASconfig @22:35:12.10 AS.seek 1ml @22:36:09.86 delay 1 second @22:36:10.88 AFV.select :holdingCoil @22:36:10.98 delay 1 second @22:36:12.01 AS.reconfigure ASfast @22:36:12.06 AS.seek 0ml @22:36:39.41 delay 3 seconds @22:36:42.44 AFV.select :carrierSolution @22:36:42.54 delay 2 seconds @22:36:44.57 AS.reconfigure ASconfig @22:36:44.63 AS.seek 1ml @22:37:42.36 delay 1 second @22:37:43.38 AFV.select :holdingCoil @22:37:43.48 delay 1 second @22:37:44.51 AS.reconfigure ASfast @22:37:44.56 AS.seek 0ml @22:38:11.89 delay 3 seconds @22:38:14.92 AFV.select :carrierSolution @22:38:15.02 delay 2 seconds @22:38:17.05 AS.reconfigure ASconfig @22:38:17.11 AS.seek 1ml @22:39:14.90 delay 1 second @22:39:15.92 AFV.select :holdingCoil @22:39:16.02 delay 1 second @22:39:17.05 AS.reconfigure ASfast @22:39:17.10 AS.seek 0ml @22:39:44.44 delay 3 seconds @22:39:47.47 AFV.select :carrierSolution @22:39:47.57 delay 2 seconds @22:39:49.60 AS.reconfigure ASconfig @22:39:49.66 AS.seek 1ml @22:40:47.43 delay 1 second @22:40:48.45 AFV.select :holdingCoil @22:40:48.55 delay 1 second @22:40:49.58 AS.reconfigure ASnorm @22:40:49.63 AS.seek 0ml @22:41:40.37 Setting up PCR for IPc2 -- writing /var/log/esp/IPc220may2422h400ml.pcr @22:41:40.40 Building PCR Slug from ASV with MM2 mix, PP3 primer @22:41:40.42 Priming with carrier @22:41:40.43 ATV.dial ARV @22:41:41.01 ARV.dial :carrier @22:41:41.36 AS.seek 0.5ml @22:42:06.95 AFV.select :carrierSolution @22:42:07.07 delay 2 seconds @22:42:09.10 AS.reconfigure ASconfig @22:42:09.15 AS.seek 1ml @22:42:38.15 delay 1 second @22:42:39.17 AFV.select :holdingCoil @22:42:39.27 delay 1 second @22:42:40.30 ATV.dial :waste @22:42:41.87 AS.reconfigure ASnorm @22:42:41.92 AS.seek :empty @22:43:32.68 AFV.select :carrierSolution @22:43:32.79 delay 2 seconds @22:43:34.82 AS.reconfigure ASconfig @22:43:34.87 AS.seek 0.5ml @22:44:03.95 delay 1 second @22:44:04.97 AFV.select :holdingCoil @22:44:05.81 Building Slug @22:44:05.82 ATV.dial :ARV @22:44:07.38 delay 2 seconds @22:44:09.41 ARV.dial :air1 @22:44:09.79 delay 2 seconds @22:44:11.88 AS.reconfigure ASslow2 @22:44:11.94 AS.seek 0.506ml @22:44:18.16 delay 2 seconds @22:44:20.19 ARV.dial :MM2 @22:44:21.60 delay 2 seconds @22:44:23.63 Adding MM2 @22:44:23.70 AS.seek 0.524ml @22:44:41.99 delay 25 seconds @22:45:07.02 ARV.dial :PP3 @22:45:07.40 Adding PP3 @22:45:07.42 delay 2 seconds @22:45:09.50 AS.seek 0.53ml @22:45:15.93 delay 10 seconds @22:45:25.96 ARV.dial :air2 @22:45:26.34 adding ARV air @22:45:26.36 delay 2 seconds @22:45:28.44 AS.seek 0.536ml @22:45:34.67 delay 5 seconds @22:45:39.70 ARV.dial :carrier @22:45:40.92 delay 7 seconds @22:45:48.02 AS.seek 0.594ml @22:46:46.49 delay 5 seconds @22:46:51.52 ATV.dial :ASV @22:46:51.92 delay 2 seconds @22:46:54.01 AS.seek 0.6ml @22:47:00.18 delay 4 seconds @22:47:04.21 ATV.dial ARV @22:47:04.73 delay 2 seconds @22:47:06.76 ARV.dial :carrier @22:47:06.77 delay 2 seconds @22:47:08.86 AS.seek 0.65ml @22:47:59.29 delay 4 seconds @22:48:03.32 ATV.dial PCR @22:48:03.90 ASV.dial :waste1 @22:48:03.91 ARV.dial :air1 @22:48:04.30 Positioning Slug by 0.324ml @22:48:04.39 AS.seek 0.326ml @22:53:30.37 delay 5 seconds @22:53:35.41 ATV.dial ARV @22:53:36.14 ASV.dial :PCRlock @22:53:36.53 delay 5 seconds @22:53:41.56 Running IPc2 ProfileIPc thermocycling @22:53:41.57 PCR.program ProfileIPc @22:53:41.68 ProfileIPc should complete in < 1:36:15 @22:53:41.69 PCR.run 2:05:18.75 @00:11:49.35CEST25-May-20 PCR.off @00:11:49.92 PCR for IPc2 completed -- closed /var/log/esp/IPc220may2422h400ml.pcr @00:11:49.93 Full MFB Decontamination @00:11:49.95 Decon extraction column and decon AIV @00:11:49.97 SPE.hold 2:46:40,55C @00:11:50.02 ASV.dial :SPEdelivery @00:11:50.67 delay 20 seconds @00:12:10.70 AFV.select :carrierSolution @00:12:10.80 delay 2 seconds @00:12:12.83 AS.reconfigure ASconfig @00:12:12.88 AS.seek 0.5ml @00:12:23.18 delay 1 second @00:12:24.20 AFV.select :holdingCoil @00:12:24.30 ATV.dial :air1 @00:12:26.19 AS.reconfigure ASslow1 @00:12:26.24 AS.seek 0.52ml @00:12:30.53 ATV.dial :ASV @00:12:32.29 ASV.dial :SPErecovery @00:12:32.99 AS.seek 0.62ml @00:12:53.40 ATV.dial :waste @00:12:55.40 AS.reconfigure ASnorm @00:12:55.45 AS.seek :empty @00:13:27.07 AFV.select :holdingCoil @00:13:27.08 ATV.dial :waste @00:13:27.10 AS.reconfigure ASconfig @00:13:27.15 AS.seek 0ml @00:13:27.46 AFV.select :carrierSolution @00:13:27.57 delay 2 seconds @00:13:29.61 AS.seek 0.5ml @00:13:58.62 delay 1 second @00:13:59.64 AFV.select :holdingCoil @00:13:59.74 ATV.dial :air3 @00:14:00.46 AS.reconfigure ASslow1 @00:14:00.51 AS.seek 0.52ml @00:14:04.82 ATV.dial :air3 @00:14:04.90 AS.seek 0.53ml @00:14:07.17 delay 2 seconds @00:14:09.19 ATV.dial :bleach @00:14:09.67 AS.seek 0.71ml @00:14:46.14 delay 3 seconds @00:14:49.17 ATV.dial :air3 @00:14:49.76 delay 3 seconds @00:14:52.85 AS.seek 0.735ml @00:14:58.15 delay 3 seconds @00:15:01.18 ATV.dial :bleach @00:15:01.83 AS.seek 0.72ml @00:15:05.14 delay 3 seconds @00:15:08.17 ATV.dial ASV @00:15:09.26 ASV.dial :SPErecovery @00:15:09.35 AS.seek 0.365ml @00:16:20.95 ATV.dial :waste @00:16:22.68 AS.reconfigure ASnorm @00:16:22.73 AS.seek :empty @00:16:41.48 ATV.dial :air3 @00:16:42.18 AS.reconfigure ASslow1 @00:16:42.23 AS.seek 0.009ml @00:16:44.62 delay 2 seconds @00:16:46.64 ATV.dial :bleach @00:16:47.11 AS.seek 0.579ml @00:18:42.03 delay 3 seconds @00:18:45.06 ATV.dial :air3 @00:18:45.61 delay 3 seconds @00:18:49.45 AS.seek 0.604ml @00:18:54.76 delay 3 seconds @00:18:57.79 ATV.dial :bleach @00:18:58.41 AS.seek 0.589ml @00:19:01.70 delay 3 seconds @00:19:04.73 ATV.dial :ASV @00:19:05.75 ASV.dial :wasteR @00:19:06.15 AS.seek 0ml @00:21:04.87 delay 1:00 @00:22:04.90 ATV.dial ASV @00:22:04.91 AFV.select :carrierSolution @00:22:05.02 delay 2 seconds @00:22:07.05 AS.reconfigure ASconfig @00:22:07.10 AS.seek 1ml @00:23:04.91 delay 1 second @00:23:05.93 AFV.select :holdingCoil @00:23:06.03 delay 1 second @00:23:07.06 ASV.dial :wasteR @00:23:07.08 AS.reconfigure ASslow1 @00:23:07.12 AS.seek 0ml @00:26:28.50 delay 10 seconds @00:26:38.53 ATV.dial :air3 @00:26:40.00 AS.seek 0.01ml @00:26:42.30 delay 2 seconds @00:26:44.32 ATV.dial :bleach @00:26:44.93 AS.seek 0.58ml @00:28:39.83 delay 3 seconds @00:28:42.86 ATV.dial :air3 @00:28:43.40 delay 3 seconds @00:28:46.49 AS.seek 0.605ml @00:28:51.81 delay 3 seconds @00:28:54.84 ATV.dial :bleach @00:28:55.45 AS.seek 0.59ml @00:28:58.74 delay 3 seconds @00:29:01.77 ATV.dial ASV @00:29:02.79 ASV.dial :SPEdelivery @00:29:03.17 AS.seek 0ml @00:31:02.07 delay 1:00 @00:32:02.10 AFV.select :carrierSolution @00:32:02.20 delay 2 seconds @00:32:04.23 AS.reconfigure ASconfig @00:32:04.28 AS.seek 1ml @00:33:02.02 delay 1 second @00:33:03.04 AFV.select :holdingCoil @00:33:03.14 delay 1 second @00:33:04.17 ATV.dial ASV @00:33:04.18 ASV.dial :SPEdelivery @00:33:04.20 AS.reconfigure ASslow1 @00:33:04.24 AS.seek 0ml @00:36:25.59 delay 10 seconds @00:36:35.62 ATV.dial :air2 @00:36:36.43 AS.reconfigure ASslow0 @00:36:36.48 AS.seek 0.8ml @00:37:57.28 delay 3 seconds @00:38:00.31 ATV.dial :ASV @00:38:01.26 ASV.dial :SPEdelivery @00:38:01.28 AS.reconfigure ASslow1 @00:38:01.33 AS.seek 0.03ml @00:40:36.45 delay 20 seconds @00:40:56.48 ATV.dial :waste,via: :air3 @00:40:58.15 AS.reconfigure ASconfig @00:40:58.20 AS.seek :empty @00:41:00.29 SPE.coast @00:41:00.37 Removing Bleach from Mixing Coil @00:41:00.39 AFV.select :carrierSolution @00:41:00.49 delay 2 seconds @00:41:02.53 AS.seek 0.5ml @00:41:31.62 delay 1 second @00:41:32.64 AFV.select :holdingCoil @00:41:32.74 ATV.dial :ASV @00:41:34.36 ASV.dial :SPErecovery @00:41:35.00 AS.reconfigure ASslow1 @00:41:35.05 AS.seek 0.73ml @00:42:21.56 ASV.dial :wasteD @00:42:22.11 AS.reconfigure ASnorm @00:42:22.17 AS.seek 0ml @00:42:59.28 AFV.select :carrierSolution @00:42:59.39 delay 2 seconds @00:43:01.42 AS.reconfigure ASconfig @00:43:01.47 AS.seek 0.5ml @00:43:30.50 delay 1 second @00:43:31.52 AFV.select :holdingCoil @00:43:31.62 ATV.dial ASV @00:43:31.63 ASV.dial :SPErecovery @00:43:32.27 AS.reconfigure ASslow0 @00:43:32.32 AS.seek 0.3ml @00:43:52.76 ATV.dial :air2 @00:43:53.77 AS.reconfigure ASslow1 @00:43:53.82 AS.seek 0.32ml @00:43:58.13 ATV.dial ASV @00:43:59.15 AS.reconfigure ASslow0 @00:43:59.20 AS.seek 0.57ml @00:44:24.64 ASV.dial :wasteD @00:44:25.95 AS.reconfigure ASnorm @00:44:26.00 AS.seek 0ml @00:44:55.06 AFV.select :carrierSolution @00:44:55.17 delay 2 seconds @00:44:57.20 AS.reconfigure ASconfig @00:44:57.25 AS.seek 0.5ml @00:45:26.27 delay 1 second @00:45:27.29 AFV.select :holdingCoil @00:45:27.39 ATV.dial ASV @00:45:27.40 ASV.dial :SPErecovery @00:45:28.05 AS.reconfigure ASslow0 @00:45:28.10 AS.seek 0.3ml @00:45:48.50 ATV.dial :air2 @00:45:49.52 AS.reconfigure ASslow1 @00:45:49.57 AS.seek 0.32ml @00:45:53.93 ATV.dial ASV @00:45:54.94 AS.reconfigure ASslow0 @00:45:54.99 AS.seek 0.57ml @00:46:20.45 ASV.dial :wasteD @00:46:21.03 AS.reconfigure ASnorm @00:46:21.07 AS.seek 0ml @00:46:50.14 ATV.dial :air2 @00:46:51.09 AS.reconfigure ASslow0 @00:46:51.14 AS.seek 0.3ml @00:47:21.69 ATV.dial :ASV @00:47:22.64 ASV.dial :wasteD @00:47:22.66 AS.reconfigure ASconfig @00:47:22.71 AS.seek 0.05ml @00:47:37.39 ATV.dial :waste @00:47:39.17 AS.seek 0ml @00:47:42.33 ASV.dial :SPEdelivery @00:47:43.30 deconLysateINT @00:47:43.31 ATV.dial :air3 @00:47:43.99 AS.reconfigure ASslow1 @00:47:44.04 AS.seek 0.01ml @00:47:46.33 delay 2 seconds @00:47:48.35 ATV.dial :bleach @00:47:48.81 AS.seek 0.96ml @00:51:00.11 delay 3 seconds @00:51:03.14 ATV.dial :air3 @00:51:03.68 delay 3 seconds @00:51:06.77 AS.seek 0.985ml @00:51:12.08 delay 3 seconds @00:51:15.11 ATV.dial :bleach @00:51:15.71 AS.seek 0.97ml @00:51:19.02 delay 3 seconds @00:51:22.05 ATV.dial AIV @00:51:23.24 AIV.dial :coilToMFB @00:51:23.25 AWV.select :waste @00:51:23.27 AFV.select :carrierSolution @00:51:23.38 delay 2 seconds @00:51:25.41 AS.reconfigure ASconfig @00:51:25.46 AS.seek 1ml @00:51:27.52 delay 1 second @00:51:28.54 AFV.select :holdingCoil @00:51:28.64 delay 1 second @00:51:29.67 AS.reconfigure ASslow0 @00:51:29.72 AS.seek :empty @00:53:10.71 AFV.select :carrierSolution @00:53:10.82 delay 2 seconds @00:53:12.85 AS.reconfigure ASconfig @00:53:12.90 AS.seek 1ml @00:54:10.74 delay 1 second @00:54:11.76 AFV.select :holdingCoil @00:54:11.86 delay 1 second @00:54:12.89 AS.reconfigure ASslow0 @00:54:12.94 AS.seek :empty @00:55:53.93 AFV.select :carrierSolution @00:55:54.04 delay 2 seconds @00:55:56.07 AS.reconfigure ASconfig @00:55:56.13 AS.seek 1ml @00:56:53.93 delay 1 second @00:56:54.95 AFV.select :holdingCoil @00:56:55.05 delay 1 second @00:56:56.08 AS.reconfigure ASslow0 @00:56:56.13 AS.seek :empty @00:58:37.10 ATV.dial :air2 @00:58:37.75 AS.reconfigure ASslow1 @00:58:37.80 AS.seek 0.009ml @00:58:40.15 AS.reconfigure ASslow0 @00:58:40.21 AS.seek 1ml @01:00:20.13 ATV.dial AIV @01:00:20.88 AS.seek 0.02ml @01:01:59.74 ATV.dial :waste @01:02:01.32 AS.seek 0ml @01:02:03.60 AWV.select :air @01:02:03.77 Cleaning PCR @01:02:03.78 AFV.select :carrierSolution @01:02:03.88 delay 2 seconds @01:02:05.91 AS.reconfigure ASconfig @01:02:06.70 AS.seek 0.5ml @01:02:35.78 delay 1 second @01:02:36.80 AFV.select :holdingCoil @01:02:36.91 ASV.dial :waste1 @01:02:37.28 ATV.dial :air3 @01:02:37.96 AS.reconfigure ASslow1 @01:02:38.01 AS.seek 0.51ml @01:02:40.29 delay 2 seconds @01:02:42.31 ATV.dial :bleach @01:02:42.77 AS.seek 0.81ml @01:03:43.39 delay 3 seconds @01:03:46.42 ATV.dial :air3 @01:03:46.96 delay 3 seconds @01:03:50.05 AS.seek 0.835ml @01:03:55.33 delay 3 seconds @01:03:58.36 ATV.dial :bleach @01:03:58.97 AS.seek 0.82ml @01:04:02.28 delay 3 seconds @01:04:05.31 ARV.dial :waste @01:04:05.90 delay 3 seconds @01:04:08.93 ATV.dial :ARV @01:04:09.78 AS.reconfigure ASconfig @01:04:09.83 AS.seek 0.47ml @01:04:30.26 AFV.select :carrierSolution @01:04:30.37 delay 2 seconds @01:04:32.41 AS.seek 1ml @01:05:03.20 delay 1 second @01:05:04.22 AFV.select :holdingCoil @01:05:04.32 delay 1 second @01:05:05.35 AS.reconfigure ASfast @01:05:05.40 AS.seek 0ml @01:05:32.75 AFV.select :carrierSolution @01:05:32.86 delay 2 seconds @01:05:34.89 AS.reconfigure ASconfig @01:05:34.94 AS.seek 1ml @01:06:32.68 delay 1 second @01:06:33.70 AFV.select :holdingCoil @01:06:33.80 delay 1 second @01:06:34.83 AS.reconfigure ASfast @01:06:34.88 AS.seek 0ml @01:07:02.23 AFV.select :carrierSolution @01:07:02.34 delay 2 seconds @01:07:04.37 AS.reconfigure ASconfig @01:07:04.42 AS.seek 1ml @01:08:02.20 delay 1 second @01:08:03.22 AFV.select :holdingCoil @01:08:03.32 delay 1 second @01:08:04.35 AS.reconfigure ASfast @01:08:04.40 AS.seek 0ml @01:08:31.72 ARV.dial between carrier and air1 @01:08:32.22 ATV.dial :air3 @01:08:33.46 AS.reconfigure ASslow1 @01:08:33.51 AS.seek 0.01ml @01:08:35.78 delay 2 seconds @01:08:37.80 ATV.dial :bleach @01:08:38.41 AS.seek 0.81ml @01:11:19.55 delay 3 seconds @01:11:22.58 ATV.dial :air3 @01:11:23.11 delay 3 seconds @01:11:26.20 AS.seek 0.835ml @01:11:31.45 delay 3 seconds @01:11:34.48 ATV.dial :bleach @01:11:35.09 AS.seek 0.82ml @01:11:38.41 delay 3 seconds @01:11:41.44 ATV.dial PCR @01:11:41.83 AS.reconfigure ASconfig @01:11:41.89 AS.seek :empty @01:12:29.34 PCR.program :bleachProfile @01:12:29.40 PCR program should complete in < 2:45 @01:12:29.41 PCR.run 10:00 @01:15:58.51 PCR.off @01:15:58.54 AFV.select :carrierSolution @01:15:58.64 delay 2 seconds @01:16:00.68 AS.seek 1ml @01:16:58.49 delay 1 second @01:16:59.51 AFV.select :holdingCoil @01:16:59.61 delay 1 second @01:17:00.64 AS.reconfigure ASfast @01:17:00.69 AS.seek 0ml @01:17:28.04 delay 3 seconds @01:17:31.07 AFV.select :carrierSolution @01:17:31.17 delay 2 seconds @01:17:33.20 AS.reconfigure ASconfig @01:17:34.00 AS.seek 1ml @01:18:31.77 delay 1 second @01:18:32.79 AFV.select :holdingCoil @01:18:32.89 delay 1 second @01:18:33.92 AS.reconfigure ASfast @01:18:33.97 AS.seek 0ml @01:19:01.29 delay 3 seconds @01:19:04.32 AFV.select :carrierSolution @01:19:04.42 delay 2 seconds @01:19:06.45 AS.reconfigure ASconfig @01:19:06.50 AS.seek 1ml @01:20:04.26 delay 1 second @01:20:05.28 AFV.select :holdingCoil @01:20:05.38 delay 1 second @01:20:06.41 AS.reconfigure ASfast @01:20:06.46 AS.seek 0ml @01:20:33.79 delay 3 seconds @01:20:36.82 AFV.select :carrierSolution @01:20:36.92 delay 2 seconds @01:20:38.95 AS.reconfigure ASconfig @01:20:39.01 AS.seek 1ml @01:21:36.79 delay 1 second @01:21:37.81 AFV.select :holdingCoil @01:21:37.91 delay 1 second @01:21:38.94 AS.reconfigure ASfast @01:21:38.99 AS.seek 0ml @01:22:06.34 delay 3 seconds @01:22:09.37 AFV.select :carrierSolution @01:22:09.47 delay 2 seconds @01:22:11.50 AS.reconfigure ASconfig @01:22:11.55 AS.seek 1ml @01:23:09.34 delay 1 second @01:23:10.36 AFV.select :holdingCoil @01:23:10.46 delay 1 second @01:23:11.49 AS.reconfigure ASnorm @01:23:11.54 AS.seek 0ml @01:24:02.25 AFV.select :carrierSolution @01:24:02.36 delay 2 seconds @01:24:04.39 AS.reconfigure ASconfig @01:24:04.44 AS.seek 1ml @01:25:02.24 delay 1 second @01:25:03.26 AFV.select :holdingCoil @01:25:03.36 delay 1 second @01:25:04.39 ATV.dial :waste @01:25:05.56 AS.reconfigure ASnorm @01:25:05.62 AS.seek 0.5ml @01:25:31.20 ATV.dial :waste2 @01:25:32.43 AS.seek 0ml @01:25:57.93 AS.coast @01:25:57.98 SS.coast @01:25:58.02 CC.coast @01:25:58.05 CS.coast @01:25:58.09 PC.coast @01:25:58.13 PS.coast @01:25:58.17 Forearm.coast @01:25:58.22 Elbow.coast @01:25:58.61 Gate.power :core,:OFF @01:25:58.63 Gate.power :raw,:OFF @01:25:58.64 Shutdown @01:25:58.65 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @10:01:42.94
Powering core bus @10:01:48.04 Gate.power :raw,:ON @10:01:48.71 SPE.coast @10:01:48.77 SPE.configure SPEconfig @10:01:48.86 AS.configure ASconfig @10:01:48.97 SS.configure SSconfig @10:01:49.07 CC.configure CCconfig @10:01:49.16 CS.configure CSconfig @10:01:49.26 PC.configure PCconfig @10:01:49.36 PS.configure PSconfig @10:01:50.08 Forearm.configure LooseForearm @10:01:50.26 Elbow.configure LooseElbow @10:01:50.39 SC.configure SCloose @10:01:50.49 SE.configure SEconfig @10:01:50.53 CH.coast @10:01:50.59 CH.configure CHconfig @10:01:50.63 PH.coast @10:01:50.69 PH.configure PHconfig @10:01:50.73 PCR.off @10:01:50.76 PCR.pidtemp= 50,0.1,0,300,25,25 @10:01:50.79 PCR.caltemp= 2.8814,0.0372,0 @10:01:50.82 All dwarf controllers running firmware version 3.75 @10:01:50.93 Valve.reset @10:01:51.10 PCR.off @10:01:51.13 PCR.pidtemp= 50,0.1,0,300,25,25 @10:01:51.17 PCR.caltemp= 2.8814,0.0372,0 @10:01:51.40 PSV.dial :air @10:01:51.42 Intake.select :close @10:01:51.46 Exhaust.select :close @10:01:51.49 CTV.dial :air @10:01:51.63
Forearm.reconfigure LooseForearm @10:01:51.70 Forearm.home.seek :retract @10:01:51.73 CSV.dial :waste1 @10:01:51.85 SSV.dial :spit,avoiding: :exhaust @10:01:51.95 PS.reconfigure PSconfig @10:01:52.04 PS.home.seek 0ml @10:01:52.51 Hand.close @10:01:52.56
Elbow.jog 1500 @10:01:52.79 CS.reconfigure CSfast @10:01:52.83 SS.reconfigure SSconfig @10:01:52.92 CS.home.seek 0ml @10:01:52.96 SS.home.seek 0ml @10:01:53.27
Elbow.reconfigure LooseElbow @10:01:53.33 Elbow.home.seek :clear @10:01:56.85 SE.reconfigure SEconfig @10:01:56.90 SE.home.seek :down @10:01:57.68 SC.reconfigure SCsloppy @10:01:57.73 SC.home.seek :initial @10:02:01.05 PS.coast @10:02:01.75
Forearm.seek :retract @10:02:01.83 Elbow.seek :hammer @10:02:03.15 Forearm.reconfigure TightForearm @10:02:03.20 Forearm.seek 5 + 350 counts @10:02:04.25 Forearm.seek :retract @10:02:05.46 PC.reconfigure PCconfig @10:02:05.48
CC.reconfigure CCconfig @10:02:05.57 PC.home.seek :open @10:02:05.60
CC.home.seek :open @10:02:09.19 Phase [stdphase] @10:02:09.21 GoodNews.email "At 10:02:09 began in tube 3: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:150\n{1 => 22, 2 => 15, 3 => 7}\nCan@10:02:09, 7.8C, 50% humidity, 14.3psia, 13.137V, 0.601A, 0.601A avg, 7.89W",Subject: "Started 20invasivedeployfinal phase stdphase" @10:02:09.23CEST25-May-20
===> STARTED Superphase (phase #15) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @10:02:09.27 Starting initialPurge @10:02:09.28 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:02:09.36 Hand.open @10:02:10.26 Elbow.reconfigure TightElbow @10:02:10.31 Elbow.seek FlushPuck::Garage @10:02:12.34 Elbow.reconfigure LooseElbow @10:02:12.47 Forearm.reconfigure LooseForearm @10:02:12.52 Forearm.seek :garage @10:02:13.56 Hand.close @10:02:14.48 Forearm.seek :retract @10:02:15.53 Elbow.reconfigure TightElbow @10:02:15.58 Elbow.seek CC @10:02:18.70 Elbow.reconfigure LooseElbow @10:02:18.91 Forearm.reconfigure TightForearm @10:02:19.01 Forearm.seek CC @10:02:20.75 CC.seek :closed @10:02:25.19 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.20 @10:02:25.42 Hand.open @10:02:26.40 Forearm.reconfigure LooseForearm @10:02:26.50 Forearm.seek :retract @10:02:27.52 Flushing the Collection Valve::Manifold @10:02:27.81 CSV.dial CSR @10:02:29.43 Pulling 5ml of CSR.flush @10:02:29.45 CSR.select :flush @10:02:29.66 CS.reconfigure CSconfig @10:02:29.76 CS.seek 5ml @10:02:43.58 delay 5 seconds @10:02:48.64 CSR.select :air @10:02:48.94 CS.reconfigure CSfast @10:02:49.08 CS.seek 7.5ml @10:02:56.09 CSV.dial :waste @10:02:57.54 CS.reconfigure CSconfig @10:02:57.67 CS.seek 0ml @10:03:17.95 Purging external Intake @10:03:18.08 CSV.dial :flush @10:03:18.57 CS.reconfigure CSfast @10:03:18.62 CS.seek 10ml @10:03:43.07 delay 5 seconds @10:03:48.10 CSV.dial CBV @10:03:48.14
CBV.dial CSV @10:03:48.96 CTV.dial :intake @10:03:49.76 Intake.select :open @10:03:49.88 CS.seek 0ml @10:04:15.89 Intake.select :close @10:04:16.00 CTV.dial :air @10:04:16.83 Flushing upper manifold @10:04:16.96 CSV.dial :flush @10:04:17.77 CS.seek 5ml @10:04:31.17 delay 5 seconds @10:04:36.20 CSV.dial :air @10:04:36.75 CS.seek 7ml @10:04:42.42 CSV.dial CBV @10:04:42.44
CBV.dial CSV @10:04:43.68 CTV.dial :air @10:04:43.70 CS.seek 0ml @10:05:01.20 delay 7 seconds @10:05:08.24 CS.seek 3ml @10:05:16.26 CSV.dial :waste @10:05:16.74 CS.seek 0ml @10:05:24.95 CSV.dial :flush @10:05:25.45 CS.seek 3ml @10:05:33.51 delay 5 seconds @10:05:38.54 CSV.dial :air @10:05:39.10 CS.seek 5ml @10:05:44.76 CSV.dial :air @10:05:44.78 CS.seek 0ml @10:05:57.51 delay 7 seconds @10:06:04.55 CS.seek 3ml @10:06:12.55 CSV.dial :waste @10:06:13.37 CS.seek 0ml @10:06:21.52 Garaging FlushPuck in Collection Clamp @10:06:21.60 Elbow.reconfigure TightElbow @10:06:21.65 Elbow.seek CC @10:06:21.91 Elbow.reconfigure LooseElbow @10:06:22.02 Forearm.seek CC @10:06:22.99 Hand.close @10:06:23.72 CC.seek :open @10:06:28.08 Forearm.seek :retract @10:06:29.07 Elbow.reconfigure TightElbow @10:06:29.12 Elbow.seek :garage @10:06:32.34 Elbow.reconfigure LooseElbow @10:06:32.44 Forearm.reconfigure TightForearm @10:06:32.49 Forearm.seek :garage @10:06:33.64 Hand.open @10:06:34.29 Forearm.reconfigure LooseForearm @10:06:34.33 Forearm.seek :retract @10:06:35.44 Starting mfb @10:06:35.45 pcrBAC sampling at most 1500ml, wcr at most 1500ml @10:06:35.49 BACfans.email "Started mfb in tube 3 at 10:06:35CEST25-May-20\n{1 => 22, 2 => 15, 3 => 7}\nCan@10:05:56, 7.7C, 50% humidity, 14.3psia, 13.087V, 0.714A, 0.714A avg, 9.34W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @10:06:35.57
Loading sh1 puck from tube 3 into Collection Clamp @10:06:35.71 Readying the MFB @10:06:35.78 Homing MFB Syringe @10:06:35.79 AFV.select :holdingCoil @10:06:36.03 ATV.dial :air2 @10:06:36.06
SC.reconfigure SCtight @10:06:36.16 SC.seek 3 @10:06:36.63 AS.reconfigure ASconfig @10:06:36.68 AS.home.seek :home @10:06:38.25
SC.reconfigure SCloose @10:06:38.53 Elbow.reconfigure TightElbow @10:06:38.58 Elbow.seek 3 @10:06:41.71 Forearm.reconfigure TightForearm @10:06:41.76 Forearm.seek 3 + 700 counts @10:06:42.23 AFV.select :carrierSolution @10:06:42.38 delay 2 seconds @10:06:42.96
SE.reconfigure SEpick @10:06:43.12 SE.seek beamBreak @10:06:44.46 AS.seek 1ml @10:06:45.86
SE.configure SEconfig @10:06:46.28 Elbow.seek 3 @10:06:46.80 Forearm.seek 3 + 300 counts @10:06:47.07 Forearm.reconfigure LooseForearm @10:06:47.19 Hand.close @10:06:47.95 SE.seek :down @10:06:48.50
Forearm.seek :retract @10:06:49.52 Elbow.seek CC @10:06:50.28 SE.coast @10:06:51.35
Elbow.reconfigure LooseElbow @10:06:51.58 Forearm.reconfigure TightForearm @10:06:51.72 Forearm.seek CC @10:06:53.48 CC.seek :closed @10:06:58.02 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.19 @10:06:58.06 Hand.open @10:06:58.92 Forearm.reconfigure LooseForearm @10:06:59.01 Forearm.seek :retract @10:07:00.07 SC.coast @10:07:00.20 collectSample 1500ml w/bubble point of 55psi @10:07:00.22 Priming 7ml @10:07:00.24 Intake.select :close @10:07:00.26 Exhaust.select :close @10:07:00.29 SS.hold @10:07:00.78 Calibrating pressure and temperature sensors @10:07:00.81 CTV.dial :air @10:07:00.84 SSV.dial :spit,avoiding: :exhaust @10:07:00.88 SS.reconfigure SSfast @10:07:01.01 SS.seek 5ml @10:07:04.00 SSV.dial CBV @10:07:04.08
CBV.dial SSV @10:07:05.61 delay 3.8 seconds @10:07:09.72 SSV.dial :spit @10:07:11.21 SS.seek 0ml @10:07:14.01 SSV.dial CBV @10:07:15.54 CTV.dial :intake @10:07:16.47 SSV.dial CBV @10:07:16.51
CBV.dial SSV @10:07:16.54 Allowing 40s for 7.0ml of remaining 7.0ml @10:07:16.56 Intake.select :open @10:07:18.88 Intake @31.9psia, vacuum from 20.0 to 24.0psi @10:07:18.92 SS.reconfigure SSinhale.with maxSpeed: 150 @10:07:19.01 SS.seek 7ml @10:07:22.90 Vacuum too high -- waiting up to 40s @10:07:25.42 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:07:25.45 SS.reconfigure SSinhale.with maxSpeed: 135 @10:07:25.56 SS.seek 7ml @10:07:29.05 Vacuum too high -- waiting up to 40s @10:07:31.27 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:07:31.31 SS.reconfigure SSinhale.with maxSpeed: 122 @10:07:31.41 SS.seek 7ml @10:07:31.68 Stroke completed -- waiting for pressure to equalize ... @10:07:34.18 Intake.select :close @10:07:34.42 SSV.dial :exhaust @10:07:35.29 Exhaust.select :open @10:07:35.46 SS.reconfigure SSexhale @10:07:35.55 SS.seek 0ml @10:07:37.42 delay 1 second @10:07:38.45 AFV.select :holdingCoil @10:07:38.58 delay 1 second @10:07:39.62 ATV.dial :waste @10:07:39.76
Exhaust.select :close @10:07:39.92 Sampling 1500.0ml @10:07:39.95 Intake.select :close @10:07:39.97 Exhaust.select :close @10:07:40.01 SS.reconfigure SSconfig @10:07:40.11 SS.hold @10:07:40.56 CTV.dial :intake @10:07:40.60 SSV.dial CBV @10:07:40.68
CBV.dial SSV @10:07:40.83 AS.reconfigure ASnorm @10:07:40.94 AS.home.seek :empty @10:07:41.57
Allowing 130s for 25.0ml of remaining 1500.0ml @10:07:41.60 Intake.select :open @10:07:43.88 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:07:43.91 SS.reconfigure SSinhale.with maxSpeed: 122 @10:07:44.01 SS.seek 25ml @10:07:47.90 Vacuum too high -- waiting up to 40s @10:07:49.76 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:07:49.78 SS.reconfigure SSinhale.with maxSpeed: 110 @10:07:49.82 SS.seek 25ml @10:07:52.90 Vacuum too high -- waiting up to 40s @10:07:54.98 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:07:54.99 SS.reconfigure SSinhale.with maxSpeed: 99 @10:07:55.04 SS.seek 25ml @10:07:58.01 Vacuum too high -- waiting up to 40s @10:08:00.08 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:08:00.10 SS.reconfigure SSinhale.with maxSpeed: 89 @10:08:00.15 SS.seek 25ml @10:08:03.14 Vacuum too high -- waiting up to 40s @10:08:05.17 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:08:05.18 SS.reconfigure SSinhale.with maxSpeed: 80 @10:08:05.23 SS.seek 25ml @10:08:08.60 Vacuum too high -- waiting up to 40s @10:08:10.48 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:08:10.50 SS.reconfigure SSinhale.with maxSpeed: 72 @10:08:10.55 SS.seek 25ml @10:08:13.86 Vacuum too high -- waiting up to 40s @10:08:15.70 SS.reconfigure SSinhale.with maxSpeed: 65 @10:08:15.75 SS.seek 25ml @10:08:19.39 Vacuum too high -- waiting up to 40s @10:08:20.98 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:08:21.00 SS.reconfigure SSinhale.with maxSpeed: 59 @10:08:21.05 SS.seek 25ml @10:08:24.93 Vacuum too high -- waiting up to 40s @10:08:26.76 SS.reconfigure SSinhale.with maxSpeed: 53 @10:08:26.81 SS.seek 25ml @10:08:28.51 Stroke completed -- waiting for pressure to equalize ... @10:08:31.73 Intake.select :close @10:08:31.89 SSV.dial :exhaust @10:08:32.16 ATV.dial :waste @10:08:32.18 ARV.dial between carrier and air1 @10:08:32.26 ASV.dial :waste1 @10:08:32.73
Exhaust.select :open @10:08:32.86 SS.reconfigure SSexhale @10:08:32.90 SS.seek 0ml @10:08:42.05 Exhaust.select :close @10:08:42.17 SSV.dial CBV @10:08:42.19
CBV.dial SSV @10:08:43.05 Allowing 130s for 25.0ml of remaining 1475.0ml @10:08:43.06 Intake.select :open @10:08:45.23 Intake @31.9psia, vacuum from 20.0 to 24.0psi @10:08:45.93 SS.reconfigure SSinhale.with maxSpeed: 53 @10:08:45.98 SS.seek 25ml @10:08:51.85 Vacuum too high -- waiting up to 40s @10:08:53.44 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:08:53.45 SS.reconfigure SSinhale.with maxSpeed: 48 @10:08:53.50 SS.seek 25ml @10:08:58.63 Vacuum too high -- waiting up to 40s @10:09:00.45 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:09:00.47 SS.reconfigure SSinhale.with maxSpeed: 43 @10:09:00.52 SS.seek 25ml @10:09:06.43 Vacuum too high -- waiting up to 40s @10:09:08.26 SS.reconfigure SSinhale.with maxSpeed: 39 @10:09:08.31 SS.seek 25ml @10:09:14.72 Vacuum too high -- waiting up to 40s @10:09:16.57 SS.reconfigure SSinhale.with maxSpeed: 35 @10:09:16.62 SS.seek 25ml @10:09:23.85 Vacuum too high -- waiting up to 40s @10:09:25.91 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:09:25.93 SS.reconfigure SSinhale.with maxSpeed: 32 @10:09:25.97 SS.seek 25ml @10:09:34.98 Vacuum too high -- waiting up to 40s @10:09:37.04 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:09:37.06 SS.reconfigure SSinhale.with maxSpeed: 29 @10:09:37.80 SS.seek 25ml @10:09:48.23 Stroke completed -- waiting for pressure to equalize ... @10:09:55.25 Intake.select :close @10:09:55.41 SSV.dial :exhaust @10:09:56.25 Exhaust.select :open @10:09:56.37 SS.reconfigure SSexhale @10:09:56.41 SS.seek 0ml @10:10:05.92 Exhaust.select :close @10:10:06.04 SSV.dial CBV @10:10:06.06
CBV.dial SSV @10:10:06.91 Allowing 130s for 25.0ml of remaining 1450.0ml @10:10:06.92 Intake.select :open @10:10:09.10 Intake @31.9psia, vacuum from 20.0 to 24.0psi @10:10:09.11 SS.reconfigure SSinhale.with maxSpeed: 29 @10:10:09.16 SS.seek 25ml @10:10:21.32 Vacuum too high -- waiting up to 40s @10:10:23.13 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:10:23.15 SS.reconfigure SSinhale.with maxSpeed: 26 @10:10:23.19 SS.seek 25ml @10:10:39.38 Vacuum too high -- waiting up to 40s @10:10:41.45 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:10:41.46 SS.reconfigure SSinhale.with maxSpeed: 23 @10:10:41.51 SS.seek 25ml @10:11:25.07 Stroke completed -- waiting for pressure to equalize ... @10:11:32.61 Intake.select :close @10:11:32.77 SSV.dial :exhaust @10:11:33.60 Exhaust.select :open @10:11:33.72 SS.reconfigure SSexhale @10:11:33.76 SS.seek 0ml @10:11:42.86 Exhaust.select :close @10:11:42.98 SSV.dial CBV @10:11:43.66
CBV.dial SSV @10:11:44.53 Allowing 130s for 25.0ml of remaining 1425.0ml @10:11:44.54 Intake.select :open @10:11:46.71 Intake @31.9psia, vacuum from 20.0 to 24.0psi @10:11:46.73 SS.reconfigure SSinhale.with maxSpeed: 23 @10:11:46.78 SS.seek 25ml @10:12:43.39 Vacuum too high -- waiting up to 40s @10:12:45.50 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:12:45.52 SS.reconfigure SSinhale.with maxSpeed: 21 @10:12:45.56 SS.seek 25ml @10:13:07.07 Stroke completed -- waiting for pressure to equalize ... @10:13:14.04 Intake.select :close @10:13:14.20 SSV.dial :exhaust @10:13:15.03 Exhaust.select :open @10:13:15.15 SS.reconfigure SSexhale @10:13:15.19 SS.seek 0ml @10:13:24.39 Exhaust.select :close @10:13:24.51 SSV.dial CBV @10:13:24.53
CBV.dial SSV @10:13:25.38 Allowing 130s for 25.0ml of remaining 1400.0ml @10:13:25.39 Intake.select :open @10:13:27.56 Intake @31.9psia, vacuum from 20.0 to 24.0psi @10:13:27.58 SS.reconfigure SSinhale.with maxSpeed: 21 @10:13:27.63 SS.seek 25ml @10:14:50.74 Stroke completed -- waiting for pressure to equalize ... @10:14:57.05 Intake.select :close @10:14:57.21 SSV.dial :exhaust @10:14:58.05 Exhaust.select :open @10:14:58.17 SS.reconfigure SSexhale @10:14:58.21 SS.seek 0ml @10:15:07.38 Exhaust.select :close @10:15:07.50 SSV.dial CBV @10:15:07.52
CBV.dial SSV @10:15:08.38 Allowing 130s for 25.0ml of remaining 1375.0ml @10:15:08.39 Intake.select :open @10:15:10.57 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:15:10.58 SS.reconfigure SSinhale.with maxSpeed: 21 @10:15:10.63 SS.seek 25ml @10:16:33.82 Stroke completed -- waiting for pressure to equalize ... @10:16:39.60 Intake.select :close @10:16:39.76 SSV.dial :exhaust @10:16:40.60 Exhaust.select :open @10:16:40.72 SS.reconfigure SSexhale @10:16:40.76 SS.seek 0ml @10:16:49.85 Exhaust.select :close @10:16:49.96 SSV.dial CBV @10:16:50.00
CBV.dial SSV @10:16:50.83 Allowing 130s for 25.0ml of remaining 1350.0ml @10:16:50.84 Intake.select :open @10:16:53.02 Intake @31.9psia, vacuum from 20.0 to 24.0psi @10:16:53.04 SS.reconfigure SSinhale.with maxSpeed: 21 @10:16:53.08 SS.seek 25ml @10:18:16.39 Stroke completed -- waiting for pressure to equalize ... @10:18:22.12 Intake.select :close @10:18:22.28 SSV.dial :exhaust @10:18:23.11 Exhaust.select :open @10:18:23.23 SS.reconfigure SSexhale @10:18:23.27 SS.seek 0ml @10:18:32.40 Exhaust.select :close @10:18:32.52 SSV.dial CBV @10:18:32.54
CBV.dial SSV @10:18:33.40 Allowing 130s for 25.0ml of remaining 1325.0ml @10:18:33.41 Intake.select :open @10:18:35.58 Intake @31.9psia, vacuum from 20.0 to 24.0psi @10:18:35.60 SS.reconfigure SSinhale.with maxSpeed: 21 @10:18:35.65 SS.seek 25ml @10:19:58.82 Stroke completed -- waiting for pressure to equalize ... @10:20:03.88 Intake.select :close @10:20:04.04 SSV.dial :exhaust @10:20:04.90 Exhaust.select :open @10:20:05.02 SS.reconfigure SSexhale @10:20:05.07 SS.seek 0ml @10:20:14.32 Exhaust.select :close @10:20:14.44 SSV.dial CBV @10:20:14.46
CBV.dial SSV @10:20:15.32 Allowing 130s for 25.0ml of remaining 1300.0ml @10:20:15.33 Intake.select :open @10:20:17.50 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:20:17.52 SS.reconfigure SSinhale.with maxSpeed: 21 @10:20:17.57 SS.seek 25ml @10:21:40.76 Stroke completed -- waiting for pressure to equalize ... @10:21:46.43 Intake.select :close @10:21:47.26 SSV.dial :exhaust @10:21:48.10 Exhaust.select :open @10:21:48.22 SS.reconfigure SSexhale @10:21:48.26 SS.seek 0ml @10:21:57.41 Exhaust.select :close @10:21:57.52 SSV.dial CBV @10:21:57.56
CBV.dial SSV @10:21:58.40 Allowing 130s for 25.0ml of remaining 1275.0ml @10:21:58.41 Intake.select :open @10:22:00.59 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:22:00.60 SS.reconfigure SSinhale.with maxSpeed: 21 @10:22:00.65 SS.seek 25ml @10:23:24.29 Stroke completed -- waiting for pressure to equalize ... @10:23:30.06 Intake.select :close @10:23:30.22 SSV.dial :exhaust @10:23:31.05 Exhaust.select :open @10:23:31.17 SS.reconfigure SSexhale @10:23:31.21 SS.seek 0ml @10:23:40.37 Exhaust.select :close @10:23:40.49 SSV.dial CBV @10:23:40.51
CBV.dial SSV @10:23:41.37 Allowing 130s for 25.0ml of remaining 1250.0ml @10:23:41.38 Intake.select :open @10:23:43.55 Intake @31.9psia, vacuum from 20.0 to 24.0psi @10:23:43.57 SS.reconfigure SSinhale.with maxSpeed: 21 @10:23:43.61 SS.seek 25ml @10:25:06.65 Stroke completed -- waiting for pressure to equalize ... @10:25:13.59 Intake.select :close @10:25:13.75 SSV.dial :exhaust @10:25:14.59 Exhaust.select :open @10:25:14.71 SS.reconfigure SSexhale @10:25:14.75 SS.seek 0ml @10:25:24.13 Exhaust.select :close @10:25:24.25 SSV.dial CBV @10:25:24.27
CBV.dial SSV @10:25:25.12 Allowing 130s for 25.0ml of remaining 1225.0ml @10:25:25.13 Intake.select :open @10:25:27.30 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:25:27.32 SS.reconfigure SSinhale.with maxSpeed: 21 @10:25:27.37 SS.seek 25ml @10:26:50.89 Stroke completed -- waiting for pressure to equalize ... @10:26:59.12 Intake.select :close @10:26:59.28 SSV.dial :exhaust @10:27:00.12 Exhaust.select :open @10:27:00.24 SS.reconfigure SSexhale @10:27:00.28 SS.seek 0ml @10:27:09.60 Exhaust.select :close @10:27:09.72 SSV.dial CBV @10:27:09.74
CBV.dial SSV @10:27:10.59 Allowing 130s for 25.0ml of remaining 1200.0ml @10:27:10.60 Intake.select :open @10:27:12.77 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:27:12.79 SS.reconfigure SSinhale.with maxSpeed: 21 @10:27:12.84 SS.seek 25ml @10:28:11.51 Vacuum too high -- waiting up to 40s @10:28:13.57 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:28:13.59 SS.reconfigure SSinhale.with maxSpeed: 19 @10:28:13.64 SS.seek 25ml @10:28:40.86 Stroke completed -- waiting for pressure to equalize ... @10:28:49.70 Intake.select :close @10:28:49.86 SSV.dial :exhaust @10:28:50.70 Exhaust.select :open @10:28:50.82 SS.reconfigure SSexhale @10:28:50.86 SS.seek 0ml @10:28:59.96 Exhaust.select :close @10:29:00.08 SSV.dial CBV @10:29:00.10
CBV.dial SSV @10:29:00.95 Allowing 130s for 25.0ml of remaining 1175.0ml @10:29:00.96 Intake.select :open @10:29:03.13 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:29:03.15 SS.reconfigure SSinhale.with maxSpeed: 19 @10:29:03.20 SS.seek 25ml @10:30:16.22 Vacuum too high -- waiting up to 40s @10:30:18.54 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:30:18.56 SS.reconfigure SSinhale.with maxSpeed: 17 @10:30:18.61 SS.seek 25ml @10:30:40.80 Stroke completed -- waiting for pressure to equalize ... @10:30:49.74 Intake.select :close @10:30:49.90 SSV.dial :exhaust @10:30:50.74 Exhaust.select :open @10:30:50.86 SS.reconfigure SSexhale @10:30:50.91 SS.seek 0ml @10:31:00.20 Exhaust.select :close @10:31:00.32 SSV.dial CBV @10:31:00.34
CBV.dial SSV @10:31:01.20 Allowing 130s for 25.0ml of remaining 1150.0ml @10:31:01.21 Intake.select :open @10:31:03.39 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:31:03.40 SS.reconfigure SSinhale.with maxSpeed: 17 @10:31:03.45 SS.seek 25ml @10:32:36.73 Vacuum too high -- waiting up to 40s @10:32:39.37 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:32:39.39 SS.reconfigure SSinhale.with maxSpeed: 15 @10:32:39.44 SS.seek 25ml @10:32:51.06 Stroke completed -- waiting for pressure to equalize ... @10:33:00.48 Intake.select :close @10:33:00.64 SSV.dial :exhaust @10:33:01.48 Exhaust.select :open @10:33:01.60 SS.reconfigure SSexhale @10:33:01.65 SS.seek 0ml @10:33:10.85 Exhaust.select :close @10:33:10.97 SSV.dial CBV @10:33:10.99
CBV.dial SSV @10:33:11.84 Allowing 130s for 25.0ml of remaining 1125.0ml @10:33:11.85 Intake.select :open @10:33:14.03 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:33:14.04 SS.reconfigure SSinhale.with maxSpeed: 15 @10:33:14.09 SS.seek 25ml @10:34:50.79 Vacuum too high -- waiting up to 40s @10:34:53.57 Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:34:53.59 SS.reconfigure SSinhale.with maxSpeed: 14 @10:34:53.64 SS.seek 25ml @10:35:16.05 Stroke completed -- waiting for pressure to equalize ... @10:35:21.86 SS.reconfigure SSconfig @10:35:21.91 SS.hold @10:35:22.04 Intake.select :close @10:35:22.20 ShallowSampler::Clogged in MAIN -- progress too slow after 400.0ml @10:35:22.21 SSV.dial :exhaust @10:35:23.06 Exhaust.select :open @10:35:23.18 SS.reconfigure SSexhale @10:35:23.22 SS.seek 0ml @10:35:32.96 Exhaust.select :close @10:35:33.07 Sampled 400.0ml @10:35:33.09 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:35:33.10 Intake.select :close @10:35:33.17 CSV.dial :waste @10:35:33.25 CS.seek 0ml @10:35:33.35 SSV.dial CBV,avoiding: :exhaust @10:35:33.37 CSV.dial :air @10:35:33.40
CBV.dial SSV @10:35:34.25 CS.seek 10ml @10:35:35.50
CTV.dial :air @10:35:36.32 delay 15 seconds @10:35:51.35 SSV.dial :spit,avoiding: :exhaust @10:35:52.80 CTV.dial CSV,avoiding: :intake @10:35:52.84
SS.seek 0ml @10:35:52.97 SSV.dial CBV,avoiding: :exhaust @10:35:59.56 CBV.dial between SSV and waste2 @10:35:59.99 CTV.dial CSV,avoiding: :air @10:36:00.01
CSV.dial CTV @10:36:00.52 CS.reconfigure CSpressurize @10:36:00.56 CS.seek 0ml @10:36:26.42 BubblePoint: Intake @70.0psia, Outlet @14.3psia @10:36:26.43 CBV.dial SSV @10:36:26.77 SS.reconfigure SSconfig @10:36:26.82 SS.seek 10ml @10:36:32.34 delay 15 seconds @10:36:47.48 Evacuated: Intake @36.3psia, Outlet @0.5psia @10:36:47.49 SSV.dial :spit,avoiding: :exhaust @10:36:48.93 SS.reconfigure SSexhale @10:36:48.98 SS.seek 0ml @10:36:54.43 SS.reconfigure SSfast @10:36:54.49 SS.seek 5ml @10:36:57.12 SSV.dial CBV,avoiding: :exhaust @10:36:57.15
CBV.dial SSV @10:36:58.57 CTV.dial :air,avoiding: :intake @10:37:00.02 CSV.dial CTV @10:37:00.74
SS.seek 10ml @10:37:01.75 CSV.dial :air @10:37:02.26 CS.reconfigure CSfast @10:37:02.31 CS.seek 0ml @10:37:03.40 CS.coast @10:37:03.46
SSV.dial :spit,avoiding: :exhaust @10:37:04.90 SS.reconfigure SSexhale @10:37:04.95 SS.seek 0ml @10:37:10.18 SS.reconfigure SSfast @10:37:10.23 SS.seek 5ml @10:37:12.79 SSV.dial CBV,avoiding: :exhaust @10:37:12.81
CBV.dial SSV @10:37:15.27 SSV.dial :spit,avoiding: :exhaust @10:37:16.70 SS.reconfigure SSconfig @10:37:16.76 SS.seek 0ml @10:37:20.10 SS.coast @10:37:20.14 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:37:20.15 Intake.select :close @10:37:20.24 CSV.dial :waste @10:37:20.37 SSV.dial CBV,avoiding: :exhaust @10:37:20.40
CBV.dial SSV @10:37:21.10 CS.seek 0ml @10:37:21.17 CSV.dial :air @10:37:21.84
CTV.dial :air @10:37:21.86 delay 15 seconds @10:37:21.97 CS.seek 10ml @10:37:36.88
SSV.dial :spit,avoiding: :exhaust @10:37:38.32 CTV.dial CSV,avoiding: :intake @10:37:38.36
SS.reconfigure SSexhale @10:37:38.46 SS.seek 0ml @10:37:38.52 SSV.dial CBV,avoiding: :exhaust @10:37:46.83 CBV.dial between SSV and waste2 @10:37:47.27 CSV.dial between air and CTV @10:37:47.60 CS.seek 8.218ml @10:37:52.82 CTV.dial CSV,avoiding: :air @10:37:52.84
CSV.dial CTV @10:37:53.21 CS.reconfigure CSpressurize @10:37:53.25 CS.seek 0ml @10:38:14.84 CSV.dial :air @10:38:15.34 CS.reconfigure CSfast @10:38:15.40 CS.seek 10ml @10:38:39.87 CSV.dial between air and CTV @10:38:40.24 CS.seek 3.051ml @10:38:57.66 CTV.dial CSV,avoiding: :air @10:38:57.68
CSV.dial CTV @10:38:58.02 CS.reconfigure CSpressurize @10:38:58.06 CS.seek 0ml @10:39:05.43 BubblePoint: Intake @69.3psia, Outlet @14.1psia @10:39:05.44 CBV.dial SSV @10:39:05.79 SS.reconfigure SSconfig @10:39:05.84 SS.seek 10ml @10:39:11.25 delay 15 seconds @10:39:26.39 Evacuated: Intake @42.8psia, Outlet @13.6psia @10:39:26.40 SSV.dial :spit,avoiding: :exhaust @10:39:27.82 SS.reconfigure SSexhale @10:39:27.88 SS.seek 0ml @10:39:32.85 SS.reconfigure SSfast @10:39:32.90 SS.seek 5ml @10:39:35.79 SSV.dial CBV,avoiding: :exhaust @10:39:36.45
CBV.dial SSV @10:39:37.95 CTV.dial :air,avoiding: :intake @10:39:39.41 CSV.dial CTV @10:39:39.45
SS.seek 10ml @10:39:40.45 CSV.dial :air @10:39:40.95 CS.reconfigure CSfast @10:39:41.00 CS.seek 0ml @10:39:42.12
SSV.dial :spit,avoiding: :exhaust @10:39:43.01 CS.coast @10:39:43.55
SS.reconfigure SSexhale @10:39:43.59 SS.seek 0ml @10:39:48.66 SS.reconfigure SSfast @10:39:48.71 SS.seek 5ml @10:39:51.41 SSV.dial CBV,avoiding: :exhaust @10:39:51.43
CBV.dial SSV @10:39:53.88 SSV.dial :spit,avoiding: :exhaust @10:39:55.31 SS.reconfigure SSconfig @10:39:55.37 SS.seek 0ml @10:39:58.53 SS.coast @10:39:58.57 Lysing: Adding 1.5ml lysis @10:39:58.58 CBV.dial between CSV and waste1,avoiding: :DBV @10:40:00.21 CTV.dial CSV @10:40:01.64 CSV.dial CSR @10:40:02.49 Pulling 1ml of CSR.lysis @10:40:02.50 CSR.select :lysis @10:40:02.62 CS.reconfigure CSconfig @10:40:02.67 CS.seek 1ml @10:40:06.28 delay 5 seconds @10:40:11.31 CSR.select :air @10:40:11.48 CS.seek 6ml @10:40:25.25 delay 2 seconds @10:40:27.27 CSV.dial :waste1 @10:40:28.67 CS.reconfigure CSfast @10:40:28.72 CS.seek :empty @10:40:43.75 CSV.dial CSR @10:40:45.21 Pulling 1.5ml of CSR.lysis @10:40:45.22 CSR.select :lysis @10:40:45.34 CS.reconfigure CSconfig @10:40:45.38 CS.seek 1.5ml @10:40:50.22 delay 5 seconds @10:40:55.25 CSR.select :air @10:40:55.43 CS.seek 4.5ml @10:41:04.06 delay 2 seconds @10:41:06.08 CSV.dial CTV @10:41:06.58 CS.seek 1.275ml @10:41:15.85 Heating sh1 puck from tube 3 in Collection Clamp to 85C for 5:00 @10:41:15.87 CH.seek 85C,8:00 @10:44:27.55 CH.hold 5:00,85C @10:49:27.72 Collection Puck Heater at 85.1C after lysing @10:49:27.74 CS.seek 0ml @10:49:32.04 CTV.dial between spare and air,avoiding: :intake @10:49:33.19 CSV.dial :air @10:49:33.69 CS.seek 5ml @10:49:47.48 CSV.dial CTV @10:49:47.98 delay 2 seconds @10:49:50.00 CSV.dial :air @10:49:50.51 delay 2 seconds @10:49:52.53 CSV.dial CTV @10:49:53.04 delay 2 seconds @10:49:55.06 CSV.dial :air @10:49:55.55 delay 2 seconds @10:49:57.58 CS.seek 0ml @10:50:11.35 Recovering Lysate into Collection syringe @10:50:11.36 CSV.dial CBV @10:50:12.48 CBV.dial CSV @10:50:12.91 CS.seek 5ml @10:50:26.63 delay 5 seconds @10:50:31.65 CTV.dial :air @10:50:32.22 CS.seek 7ml @10:50:38.32 delay 5 seconds @10:50:43.34 CSV.dial :air2 @10:50:43.83 CS.seek 10ml @10:50:52.54 CSV.dial between air and CTV,avoiding: CBV @10:50:53.81 Lysing Complete @10:50:53.89 Storing sh1 puck in Collection Clamp to tube 2 @10:50:54.08 SC.reconfigure SCtight @10:50:54.13 SC.seek 2 @10:50:56.34 SC.reconfigure SCloose @10:50:56.56 Elbow.reconfigure TightElbow @10:50:56.61 Elbow.seek 2 @10:50:58.59 Forearm.reconfigure TightForearm @10:50:58.64 Forearm.seek 2 + 700 counts @10:50:59.70 SE.reconfigure SEpick @10:50:59.78 SE.seek beamBreak @10:51:01.60 SE.configure SEconfig @10:51:02.46 Elbow.reconfigure LimpElbow @10:51:02.51 Forearm.reconfigure LooseForearm @10:51:02.56 Forearm.seek 2 @10:51:02.87 Elbow.hold @10:51:02.94 Elbow.reconfigure TightElbow @10:51:03.05 SE.seek 14.08 Pucks below Top Plate @10:51:03.54 Forearm.force -20 @10:51:03.58 Forearm.seek 2 + 331 counts @10:51:03.91 Forearm.reconfigure TightForearm @10:51:03.96 Forearm.seek 2 + 700 counts @10:51:04.33 SE.seek 15.08 Pucks below Top Plate @10:51:04.95 Forearm.reconfigure LooseForearm @10:51:05.00 Forearm.seek :retract @10:51:05.90 Elbow.seek CC @10:51:07.55 Elbow.reconfigure LooseElbow @10:51:07.66 Forearm.seek CC @10:51:08.73 Hand.close @10:51:09.43 CC.seek :open @10:51:13.93 Forearm.seek :retract @10:51:14.97 Elbow.reconfigure TightElbow @10:51:15.02 Elbow.seek 2 @10:51:16.95 Elbow.reconfigure LimpElbow @10:51:17.01 Forearm.seek 2 @10:51:18.02 Elbow.hold @10:51:18.09 Elbow.reconfigure TightElbow @10:51:18.14 Hand.open @10:51:18.94 Forearm.force -20 @10:51:18.98 Forearm.seek 2 + 346 counts @10:51:19.32 Forearm.reconfigure TightForearm @10:51:19.36 Forearm.seek 2 + 700 counts @10:51:19.73 SE.seek :down @10:51:20.90
Forearm.reconfigure LooseForearm @10:51:20.95 Forearm.seek :retract @10:51:21.10 SE.coast @10:51:21.87
SC.coast @10:51:21.91 Tranferring lysate to PRV @10:51:21.92 CSV.dial :air @10:51:22.25 CS.seek 10ml @10:51:22.32 CSV.dial between air and CTV @10:51:22.67 AIV.dial :bypass @10:51:22.87 Beginning samplePassC @10:51:22.89 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:51:22.96 Hand.open @10:51:23.31 Elbow.seek FlushPuck::Garage @10:51:25.36 Elbow.reconfigure LooseElbow @10:51:26.06 Forearm.seek :garage @10:51:27.16 Hand.close @10:51:27.94 Forearm.seek :retract @10:51:28.96 Elbow.reconfigure TightElbow @10:51:29.01 Elbow.seek CC @10:51:31.93 Elbow.reconfigure LooseElbow @10:51:32.04 Forearm.reconfigure TightForearm @10:51:32.09 Forearm.seek CC @10:51:33.50 CC.seek :closed @10:51:37.76 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.11 @10:51:37.84 Hand.open @10:51:38.50 Forearm.reconfigure LooseForearm @10:51:38.55 Forearm.seek :retract @10:51:39.54 AWV.select :air @10:51:39.55 AIV.dial :bypass @10:51:39.56 PSV.dial PSR @10:51:41.02 Pulling 1.5ml of PSR.flush @10:51:41.03 PSR.select :flush @10:51:41.16 PS.seek 1.5ml @10:51:57.27 delay 5 seconds @10:52:02.30 PSV.dial :waste1 @10:52:02.81 PS.seek 0ml @10:52:18.80 PSV.dial PSR @10:52:19.30 PSR.select :air @10:52:19.42 PS.seek 2.5ml @10:52:45.53 PRV.dial CBV @10:52:45.57
CBV.dial PRV @10:52:46.43 PSV.dial :mixloop @10:52:47.52 CTV.dial :air,avoiding: :intake @10:52:47.53 PTV.dial :PRVmixing @10:52:47.62 PS.seek 0ml @10:53:13.71 PSV.dial :air @10:53:14.22 PS.seek 2.5ml @10:53:40.37 PSV.dial :mixloop @10:53:40.88 PS.seek 0ml @10:54:06.96 PSV.dial :air @10:54:07.45 PS.seek 2.5ml @10:54:33.62 PSV.dial :mixloop @10:54:34.13 PS.seek 0ml @10:55:00.24 Garaging FlushPuck in Collection Clamp @10:55:00.32 Elbow.reconfigure TightElbow @10:55:00.37 Elbow.seek CC @10:55:00.62 Elbow.reconfigure LooseElbow @10:55:00.73 Forearm.seek CC @10:55:01.78 Hand.close @10:55:02.56 CC.seek :open @10:55:06.93 Forearm.seek :retract @10:55:07.95 Elbow.reconfigure TightElbow @10:55:08.00 Elbow.seek :garage @10:55:11.73 Elbow.reconfigure LooseElbow @10:55:11.86 Forearm.reconfigure TightForearm @10:55:11.91 Forearm.seek :garage @10:55:13.09 Hand.open @10:55:13.88 Forearm.reconfigure LooseForearm @10:55:13.93 Forearm.seek :retract @10:55:14.97 Connecting PS to CS to transfer sample @10:55:14.98 CBV.dial AIV @10:55:14.99 AIV.dial :bypass @10:55:15.01 PSV.dial :mixloop @10:55:15.02 PTV.dial :PRVmixing @10:55:15.03 PRV.dial AIV @10:55:15.05 CTV.dial :air,avoiding: :intake @10:55:15.06 Loading lyfil puck from tube 3 into Collection Clamp @10:55:15.32 SC.reconfigure SCtight @10:55:15.37 SC.seek 3 @10:55:17.55 SC.reconfigure SCloose @10:55:17.78 Elbow.reconfigure TightElbow @10:55:17.83 Elbow.seek 3 @10:55:20.03 Forearm.reconfigure TightForearm @10:55:20.08 Forearm.seek 3 + 700 counts @10:55:21.13 SE.reconfigure SEpick @10:55:21.21 SE.seek beamBreak @10:55:23.87 SE.configure SEconfig @10:55:24.04 Elbow.seek 3 @10:55:24.41 Forearm.seek 3 + 300 counts @10:55:24.70 Forearm.reconfigure LooseForearm @10:55:24.74 Hand.close @10:55:25.33 SE.seek :down @10:55:25.56
Forearm.seek :retract @10:55:26.54 Elbow.seek CC @10:55:27.72 SE.coast @10:55:28.15
Elbow.reconfigure LooseElbow @10:55:28.25 Forearm.reconfigure TightForearm @10:55:28.30 Forearm.seek CC @10:55:29.69 CC.seek :closed @10:55:33.95 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.18 @10:55:33.96 Hand.open @10:55:34.77 Forearm.reconfigure LooseForearm @10:55:34.81 Forearm.seek :retract @10:55:35.84 SC.coast @10:55:35.88 CTV.dial CSV,avoiding: :intake @10:55:37.33 CSV.dial CTV @10:55:37.68 CS.seek 5ml @10:55:51.48 delay 1:20 @10:57:11.51 CS.seek 2.5ml @10:57:18.89 delay 40 seconds @10:57:58.93 CS.seek 0ml @10:58:06.52 delay 1:00 @10:59:06.55 PSV.dial :air @10:59:07.05 PS.seek 2.5ml @10:59:33.21 PSV.dial :mixloop @10:59:33.68 delay 3 seconds @10:59:36.70 PSV.dial :air @10:59:37.87 delay 2 seconds @10:59:39.89 PSV.dial :mixloop @10:59:40.37 delay 3 seconds @10:59:43.39 PSV.dial :waste1 @10:59:44.78 PS.seek :empty @11:00:11.00 CBV.dial :waste1 @11:00:11.50 delay 5 seconds @11:00:16.53 CBV.dial AIV @11:00:17.04 delay 10 seconds @11:00:27.07 CBV.dial :waste1 @11:00:27.58 delay 10 seconds @11:00:37.61 CBV.dial AIV @11:00:38.12 delay 10 seconds @11:00:48.15 CTV.dial CSV,avoiding: :intake @11:00:48.16 CBV.dial between AIV and waste1 @11:00:48.52 CSV.dial :air @11:00:49.03 CS.seek 10ml @11:01:15.65 CSV.dial CTV @11:01:16.16 CS.seek 0ml @11:01:42.68 CBV.dial :waste1 @11:01:43.01 delay 10 seconds @11:01:53.04 CTV.dial :air,avoiding: :intake @11:01:54.56 Storing lyfil puck in Collection Clamp to tube 2 @11:01:54.75 SC.reconfigure SCtight @11:01:54.80 SC.seek 2 @11:01:57.02 SC.reconfigure SCloose @11:01:57.24 Elbow.reconfigure TightElbow @11:01:57.29 Elbow.seek 2 @11:01:59.34 Forearm.reconfigure TightForearm @11:01:59.39 Forearm.seek 2 + 700 counts @11:02:00.44 SE.reconfigure SEpick @11:02:00.52 SE.seek beamBreak @11:02:02.23 SE.configure SEconfig @11:02:02.40 Elbow.reconfigure LimpElbow @11:02:02.45 Forearm.reconfigure LooseForearm @11:02:02.50 Forearm.seek 2 @11:02:02.79 Elbow.hold @11:02:02.86 Elbow.reconfigure TightElbow @11:02:02.97 SE.seek 15.09 Pucks below Top Plate @11:02:03.41 Forearm.force -20 @11:02:03.45 Forearm.seek 2 + 341 counts @11:02:03.78 Forearm.reconfigure TightForearm @11:02:03.83 Forearm.seek 2 + 700 counts @11:02:04.13 SE.seek 16.09 Pucks below Top Plate @11:02:04.79 Forearm.reconfigure LooseForearm @11:02:04.84 Forearm.seek :retract @11:02:05.74 Elbow.seek CC @11:02:07.46 Elbow.reconfigure LooseElbow @11:02:07.57 Forearm.seek CC @11:02:08.56 Hand.close @11:02:09.20 CC.seek :open @11:02:13.68 Forearm.seek :retract @11:02:14.70 Elbow.reconfigure TightElbow @11:02:14.75 Elbow.seek 2 @11:02:16.65 Elbow.reconfigure LimpElbow @11:02:17.31 Forearm.seek 2 @11:02:18.39 Elbow.hold @11:02:18.48 Elbow.reconfigure TightElbow @11:02:18.53 Hand.open @11:02:19.33 Forearm.force -20 @11:02:19.37 Forearm.seek 2 + 339 counts @11:02:19.71 Forearm.reconfigure TightForearm @11:02:19.75 Forearm.seek 2 + 700 counts @11:02:20.14 SE.seek :down @11:02:21.30
Forearm.reconfigure LooseForearm @11:02:21.38 SE.coast @11:02:21.40
Forearm.seek :retract @11:02:22.39 SC.coast @11:02:22.42 PSV.dial :air @11:02:23.59 PS.seek 0.499ml @11:02:29.47 delay 3 seconds @11:02:32.49 PSV.dial :mixloop @11:02:32.97 PRV.dial :air @11:02:33.48 delay 3 seconds @11:02:36.51 PRV.dial CBV @11:02:37.01 CBV.dial AIV @11:02:37.52 PRV.dial between CBV and air @11:02:37.87 PRV.dial :air @11:02:38.27 PS.reconfigure PSslow @11:02:38.32 PS.seek 0.599ml @11:02:40.81 PRV.dial :waste2 @11:02:41.60 PS.reconfigure PSconfig @11:02:41.65 PS.seek :empty @11:02:48.50 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:02:48.57 Hand.open @11:02:48.95 Elbow.seek FlushPuck::Garage @11:02:50.84 Elbow.reconfigure LooseElbow @11:02:50.95 Forearm.seek :garage @11:02:51.96 Hand.close @11:02:52.70 Forearm.seek :retract @11:02:53.76 Elbow.reconfigure TightElbow @11:02:53.81 Elbow.seek CC @11:02:56.76 Elbow.reconfigure LooseElbow @11:02:56.87 Forearm.reconfigure TightForearm @11:02:56.92 Forearm.seek CC @11:02:58.27 CC.seek :closed @11:03:02.52 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.08 @11:03:02.60 Hand.open @11:03:03.27 Forearm.reconfigure LooseForearm @11:03:03.32 Forearm.seek :retract @11:03:04.42 Clearing PML for modified lysate @11:03:05.12 CTV.dial :air,avoiding: :intake @11:03:05.14 PRV.dial :waste2 @11:03:05.15 PTV.dial :PRV,avoiding: :puck @11:03:05.97 delay 2 seconds @11:03:07.99 PTV.dial :PRVmixing,avoiding: :puck @11:03:08.80 delay 2 seconds @11:03:10.82 PSV.dial :PML @11:03:10.83 delay 2 seconds @11:03:12.86 PRV.dial :air @11:03:13.67 delay 2 seconds @11:03:15.75 PS.seek 1.499ml @11:03:31.84 delay 5 seconds @11:03:36.88 PS.seek 2.5ml @11:03:47.81 PSV.dial :air @11:03:48.31 PS.seek 0ml @11:04:14.47 mixing lysate with speDil @11:04:14.48 PSV.dial :PML @11:04:14.95 CBV.dial :PRV @11:04:14.96 PRV.dial :air @11:04:14.98 delay 2 seconds @11:04:17.00 PRV.dial :speDil @11:04:17.50 delay 2 seconds @11:04:19.59 PS.reconfigure PSslow1 @11:04:19.64 PS.seek 0.225ml @11:04:43.22 delay 5 seconds @11:04:48.25 PRV.dial CBV @11:04:49.08 delay 2 seconds @11:04:51.16 PS.seek 0.475ml @11:05:17.00 delay 5 seconds @11:05:22.03 PRV.dial :air @11:05:22.53 delay 2 seconds @11:05:24.56 PS.seek 0.965ml @11:06:14.50 delay 10 seconds @11:06:24.53 PRV.dial CBV @11:06:25.02 CTV.dial :air,avoiding: :intake @11:06:25.03 PSV.dial :air @11:06:25.64 PS.reconfigure PSconfig @11:06:25.69 PS.seek 2.5ml @11:06:42.10 delay 3 seconds @11:06:45.12 PSV.dial PTV @11:06:45.89 PTV.dial :PRV,avoiding: :puck @11:06:46.72 PS.seek 0ml @11:07:12.82 PSV.dial :air @11:07:13.62 PS.seek 2.5ml @11:07:39.82 delay 3 seconds @11:07:42.84 PSV.dial PTV @11:07:43.63 PTV.dial :PRV,avoiding: :puck @11:07:43.65 PS.seek 0ml @11:08:09.72 Pushing modified lysate into AIV Injection Loop @11:08:09.73 CBV.dial AIV @11:08:09.74 AIV.dial :bypass @11:08:09.76 PRV.dial CBV @11:08:09.77 PSV.dial :air @11:08:10.58 PS.seek 2.5ml @11:08:36.79 delay 3 seconds @11:08:39.81 PSV.dial :PML @11:08:40.29 PTV.dial :PRVmixing,avoiding: :puck @11:08:41.17 PS.reconfigure PSslow1 @11:08:41.22 PS.seek 1.74ml @11:09:58.54 delay 5 seconds @11:10:03.57 AIV.dial :sample @11:10:04.17 AWV.select :waste @11:10:04.34 PS.reconfigure PSslow2 @11:10:04.39 PS.seek 1.22ml @11:11:51.38 delay 15 seconds @11:12:06.41 AIV.dial :bypass @11:12:07.02 PS.reconfigure PSconfig @11:12:07.07 PS.seek 0ml @11:12:20.26 PSV.dial :air @11:12:20.75 PS.seek 2.5ml @11:12:46.92 PSV.dial :PML @11:12:47.43 PS.seek 0ml @11:13:13.57 AIV.dial :coilToMFB @11:13:13.58 AWV.select :air @11:13:14.42 Extracting Nucleic Acid from AIV @11:13:14.45
Beginning samplePassD @11:13:14.48 SPE.hold 2:46:40,55C @11:13:14.57 ASV.dial :SPEdelivery @11:13:14.65
AWV.select :air @11:13:14.66 AIV.dial :bypass @11:13:14.68 PSV.dial PSR @11:13:15.00 delay 20 seconds @11:13:15.89
Pulling 1.5ml of PSR.killMFB @11:13:15.90 PSR.select :killMFB @11:13:16.02 PS.seek 1.5ml @11:13:32.10 delay 5 seconds @11:13:35.03 primeLysateINT @11:13:35.04 AWV.select :air @11:13:35.05 AIV.dial :coilToMFB @11:13:35.07 ATV.dial AIV @11:13:36.90 AS.reconfigure ASslow1 @11:13:36.95 AS.seek 0.299ml @11:13:37.12
PSV.dial :waste1 @11:13:37.63 PS.seek 0ml @11:13:53.64 PSV.dial PSR @11:13:54.15 PSR.select :air @11:13:54.28 PS.seek 2.5ml @11:14:20.42 PRV.dial CBV @11:14:20.45
CBV.dial PRV @11:14:20.47 PSV.dial :mixloop @11:14:21.62 CTV.dial :air,avoiding: :intake @11:14:21.63 PTV.dial :PRVmixing @11:14:21.66 PS.seek 0ml @11:14:37.59 delay 10 seconds @11:14:47.62 ATV.dial :air2 @11:14:47.82
PSV.dial PSR @11:14:48.49 AS.seek 0.329ml @11:14:49.03
Pulling 1.5ml of PSR.flush PSR.select :flush @11:14:49.16 PS.seek 1.5ml @11:14:54.78 ATV.dial :waste2 @11:14:55.36 AS.reconfigure ASnorm @11:14:55.41 AS.seek :empty @11:15:05.17
delay 5 seconds @11:15:10.20 PSV.dial :waste1 @11:15:10.72 PS.seek 0ml @11:15:12.41 Lysate primed! @11:15:12.42 AFV.select :holdingCoil @11:15:12.43 ATV.dial :air2 @11:15:13.09 AS.reconfigure ASslow1 @11:15:13.14 AS.seek 0.069ml @11:15:26.70
PSV.dial PSR @11:15:27.21 PSR.select :air @11:15:27.34 PS.seek 2.5ml @11:15:27.47 delay 3 seconds @11:15:30.49 ATV.dial :AIV @11:15:31.38 AS.seek 0.469ml @11:15:53.45 PRV.dial CBV @11:15:53.48
CBV.dial PRV @11:15:53.49 PSV.dial :mixloop @11:15:54.75 CTV.dial :air,avoiding: :intake @11:15:54.76 PTV.dial :PRVmixing @11:15:54.78 PS.seek 0ml @11:16:20.86 PSV.dial PSR @11:16:22.06 Pulling 1.5ml of PSR.flush @11:16:22.07 PSR.select :flush @11:16:22.20 PS.seek 1.5ml @11:16:38.29 delay 5 seconds @11:16:43.32 PSV.dial :waste1 @11:16:43.84 PS.seek 0ml @11:16:52.05 delay 5 seconds @11:16:57.14 Solid Phase Extraction Heater at 44.1C @11:16:57.15 ATV.dial ASV @11:16:57.56 ASV.dial :SPEdelivery @11:16:57.65 AS.seek 0.041ml @11:16:59.92
PSV.dial PSR @11:17:00.42 PSR.select :air @11:17:00.55 PS.seek 2.5ml @11:17:26.64 PRV.dial CBV @11:17:26.67
CBV.dial PRV @11:17:26.68 PSV.dial :mixloop @11:17:28.48 CTV.dial :air,avoiding: :intake @11:17:28.49 PTV.dial :PRVmixing @11:17:28.52 PS.seek 0ml @11:17:54.64 PSV.dial PSR @11:17:55.83 Pulling 1.5ml of PSR.flush PSR.select :flush @11:17:55.96 PS.seek 1.5ml @11:18:11.98 delay 5 seconds @11:18:17.01 PSV.dial :waste1 @11:18:17.53 PS.seek 0ml @11:18:24.00 delay 10 seconds @11:18:33.52
PSV.dial PSR @11:18:34.03 PSR.select :air @11:18:34.08 rinseLysateLineINT @11:18:34.09 AFV.select :carrierSolution @11:18:34.15
PS.seek 2.5ml @11:18:34.21 delay 2 seconds @11:18:36.23 AS.reconfigure ASconfig @11:18:36.28 AS.seek 1ml @11:19:00.30 PRV.dial CBV @11:19:00.33
CBV.dial PRV @11:19:00.35 PSV.dial :mixloop @11:19:01.47 CTV.dial :air,avoiding: :intake @11:19:01.48 PTV.dial :PRVmixing @11:19:01.50 PS.reconfigure PSslow @11:19:01.55 PS.seek 1.2ml @11:19:28.36 PSV.dial :waste1 @11:19:29.81 PS.reconfigure PSconfig @11:19:29.85 PS.seek 0ml @11:19:31.72 delay 1 second @11:19:32.74 AFV.select :holdingCoil @11:19:32.84 delay 1 second @11:19:33.87 ATV.dial AIV @11:19:34.43 AIV.dial :coilToMFB @11:19:34.44 AWV.select :waste @11:19:34.56 AS.reconfigure ASslow0 @11:19:34.60 AS.seek 0ml @11:19:42.80
PSV.dial :air @11:19:43.93 PS.seek 2.5ml @11:20:10.11 PSV.dial :mixloop @11:20:10.59 PS.reconfigure PSslow @11:20:10.63 PS.seek 0ml @11:21:01.77 PSV.dial :air @11:21:02.25 PS.reconfigure PSconfig @11:21:02.30 PS.seek 2.5ml @11:21:15.50 ATV.dial :air2 @11:21:16.17 AS.reconfigure ASslow1 @11:21:16.22 AS.seek 0.05ml @11:21:26.60 ATV.dial :AIV @11:21:27.43 AS.seek 0.01ml @11:21:28.39
PSV.dial :mixloop @11:21:28.87 PS.reconfigure PSslow @11:21:28.91 PS.seek 0ml @11:21:35.77 ATV.dial :waste @11:21:37.40 AS.seek :empty @11:21:39.82 ethanol wash @11:21:39.83 ATV.dial :air2 @11:21:41.09 AS.reconfigure ASslow2 @11:21:41.14 AS.seek 0.009ml @11:21:51.74 delay 3 seconds @11:21:54.77 Pulling 0.4ml of Ethanol Wash @11:21:54.78 ATV.dial :ethanol @11:21:55.59 AS.reconfigure ASslow1 @11:21:55.64 AS.seek 0.409ml @11:22:19.96
Cleaning Collection Syringe @11:22:19.97 CSV.dial CSR @11:22:20.51 Pulling 4ml of CSR.flush @11:22:20.52 CSR.select :flush @11:22:20.65 CS.seek 4ml @11:22:31.86 delay 5 seconds @11:22:36.89 CSR.select :air @11:22:37.02 CS.seek 10ml @11:22:53.58 CSV.dial CTV @11:22:53.62
CTV.dial CSV @11:22:55.14 CBV.dial :waste1 @11:22:55.65 CS.seek 0ml @11:23:16.33 delay 3 seconds @11:23:19.36 ATV.dial :air1 @11:23:19.77 delay 3 seconds @11:23:22.11
CSV.dial CSR @11:23:22.92 AS.seek 0.434ml @11:23:23.37
Pulling 4ml of CSR.flush @11:23:23.38 CSR.select :flush @11:23:23.50 CS.seek 4ml @11:23:28.24 delay 3 seconds @11:23:31.27 ATV.dial :ethanol @11:23:31.89 AS.seek 0.419ml @11:23:34.68
delay 5 seconds @11:23:35.18 delay 3 seconds @11:23:38.21 ATV.dial ASV @11:23:39.51 AS.seek 0.092ml @11:23:39.71
CSR.select :air @11:23:39.84 CS.seek 10ml @11:23:56.14 CSV.dial CTV @11:23:56.17
CTV.dial CSV @11:23:56.71 CBV.dial :waste1 @11:23:56.73 CS.seek 0ml @11:24:23.31 Garaging FlushPuck in Collection Clamp @11:24:23.39 Elbow.reconfigure TightElbow @11:24:23.44 Elbow.seek CC @11:24:23.70 Elbow.reconfigure LooseElbow @11:24:23.80 Forearm.seek CC @11:24:24.83 Hand.close @11:24:25.49 CC.seek :open @11:24:29.90 Forearm.seek :retract @11:24:30.92 Elbow.reconfigure TightElbow @11:24:30.97 Elbow.seek :garage @11:24:34.13 Elbow.reconfigure LooseElbow @11:24:34.24 Forearm.reconfigure TightForearm @11:24:34.29 Forearm.seek :garage @11:24:35.46 Hand.open @11:24:36.31 Forearm.reconfigure LooseForearm @11:24:36.36 Forearm.seek :retract @11:24:37.39 Whole Cell Archive sampling 1500ml @11:24:37.41 Loading wcr puck from tube 3 into Collection Clamp @11:24:37.67 SC.reconfigure SCtight @11:24:37.72 SC.seek 3 @11:24:39.88 SC.reconfigure SCloose @11:24:40.11 Elbow.reconfigure TightElbow @11:24:40.16 Elbow.seek 3 @11:24:42.28 Forearm.reconfigure TightForearm @11:24:42.33 Forearm.seek 3 + 700 counts @11:24:43.37 SE.reconfigure SEpick @11:24:43.46 SE.seek beamBreak @11:24:45.52 delay 3 seconds @11:24:46.24 SE.configure SEconfig @11:24:46.41 Elbow.seek 3 @11:24:46.69 Forearm.seek 3 + 300 counts @11:24:46.96 Forearm.reconfigure LooseForearm @11:24:47.01 Hand.close @11:24:47.76 SE.seek :down @11:24:48.68 Forearm.seek :retract @11:24:48.76 ATV.dial :waste2 @11:24:49.51 AS.reconfigure ASconfig @11:24:49.56 AS.seek :empty @11:24:49.64 Elbow.seek CC @11:24:50.24 SE.coast @11:24:51.36 Elbow.reconfigure LooseElbow @11:24:51.47 Forearm.reconfigure TightForearm @11:24:51.52 Forearm.seek CC @11:24:52.87 CC.seek :closed @11:24:55.24 ATV.dial :air2 @11:24:55.63 AS.reconfigure ASslow1 @11:24:55.71 AS.seek 1ml @11:24:57.14 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.23 @11:24:57.15 Hand.open @11:24:57.94 Forearm.reconfigure LooseForearm @11:24:57.99 Forearm.seek :retract @11:24:59.08 SC.coast @11:24:59.12 collectSample 1500ml w/bubble point of 55psi @11:24:59.14 Priming 7ml @11:24:59.15 Intake.select :close @11:24:59.16 Exhaust.select :close @11:24:59.17 SS.hold @11:24:59.41 Calibrating pressure sensors @11:24:59.42 CTV.dial :air @11:25:00.86 SSV.dial :spit,avoiding: :exhaust @11:25:00.88 SS.reconfigure SSfast @11:25:00.94 SS.seek 5ml @11:25:03.59 SSV.dial CBV @11:25:03.62 CBV.dial SSV @11:25:05.19 delay 3.8 seconds @11:25:09.14 SSV.dial :spit @11:25:10.60 SS.seek 0ml @11:25:13.45 SSV.dial CBV @11:25:14.89 CTV.dial :intake @11:25:15.72 SSV.dial CBV @11:25:15.74 CBV.dial SSV @11:25:15.76 Allowing 40s for 7.0ml of remaining 7.0ml @11:25:15.77 Intake.select :open @11:25:17.94 Intake @32.2psia, vacuum from 20.0 to 24.0psi @11:25:17.96 SS.reconfigure SSinhale.with maxSpeed: 150 @11:25:18.01 SS.seek 7ml @11:25:23.83 Stroke completed -- waiting for pressure to equalize ... @11:25:38.01 Intake.select :close @11:25:38.17 SSV.dial :exhaust @11:25:39.04 Exhaust.select :open @11:25:39.16 SS.reconfigure SSexhale @11:25:39.21 SS.seek 0ml @11:25:43.08 Exhaust.select :close @11:25:43.19 Sampling 1500.0ml @11:25:43.21 Intake.select :close @11:25:43.22 Exhaust.select :close @11:25:43.23 SS.reconfigure SSconfig @11:25:43.28 SS.hold @11:25:43.51 CTV.dial :intake @11:25:43.54 SSV.dial CBV @11:25:43.56 CBV.dial SSV @11:25:44.44 Allowing 130s for 25.0ml of remaining 1500.0ml @11:25:44.45 Intake.select :open @11:25:46.64 Intake @32.0psia, vacuum from 20.0 to 24.0psi @11:25:46.66 SS.reconfigure SSinhale.with maxSpeed: 150 @11:25:46.70 SS.seek 25ml @11:25:50.41 Vacuum too high -- waiting up to 40s @11:25:53.05 Intake @32.1psia, vacuum from 20.0 to 24.0psi @11:25:53.07 SS.reconfigure SSinhale.with maxSpeed: 135 @11:25:53.12 SS.seek 25ml @11:25:56.00 Vacuum too high -- waiting up to 40s @11:25:58.39 SS.reconfigure SSinhale.with maxSpeed: 122 @11:25:58.44 SS.seek 25ml @11:26:01.24 Vacuum too high -- waiting up to 40s @11:26:03.60 Intake @32.1psia, vacuum from 20.0 to 24.0psi @11:26:03.62 SS.reconfigure SSinhale.with maxSpeed: 110 @11:26:03.66 SS.seek 25ml @11:26:07.14 Vacuum too high -- waiting up to 40s @11:26:08.77 Intake @32.0psia, vacuum from 20.0 to 24.0psi @11:26:08.79 SS.reconfigure SSinhale.with maxSpeed: 99 @11:26:08.83 SS.seek 25ml @11:26:11.32 Vacuum too high -- waiting up to 40s @11:26:13.48 SS.reconfigure SSinhale.with maxSpeed: 89 @11:26:13.53 SS.seek 25ml @11:26:16.31 Vacuum too high -- waiting up to 40s @11:26:18.43 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:26:18.45 SS.reconfigure SSinhale.with maxSpeed: 80 @11:26:18.50 SS.seek 25ml @11:26:21.03 Vacuum too high -- waiting up to 40s @11:26:23.18 Intake @32.0psia, vacuum from 20.0 to 24.0psi @11:26:23.20 SS.reconfigure SSinhale.with maxSpeed: 72 @11:26:23.25 SS.seek 25ml @11:26:25.97 Vacuum too high -- waiting up to 40s @11:26:28.10 Intake @32.0psia, vacuum from 20.0 to 24.0psi @11:26:28.12 SS.reconfigure SSinhale.with maxSpeed: 65 @11:26:28.17 SS.seek 25ml @11:26:31.44 Vacuum too high -- waiting up to 40s @11:26:33.77 Intake @32.0psia, vacuum from 20.0 to 24.0psi @11:26:33.79 SS.reconfigure SSinhale.with maxSpeed: 59 @11:26:33.83 SS.seek 25ml @11:26:35.41 Stroke completed -- waiting for pressure to equalize ... @11:26:39.29 Intake.select :close @11:26:39.45 SSV.dial :exhaust @11:26:40.30 Exhaust.select :open @11:26:40.42 SS.reconfigure SSexhale @11:26:40.47 SS.seek 0ml @11:26:49.80 Exhaust.select :close @11:26:49.92 SSV.dial CBV @11:26:49.96 CBV.dial SSV @11:26:50.80 Allowing 130s for 25.0ml of remaining 1475.0ml @11:26:50.81 Intake.select :open @11:26:52.99 Intake @32.0psia, vacuum from 20.0 to 24.0psi @11:26:53.01 SS.reconfigure SSinhale.with maxSpeed: 59 @11:26:53.06 SS.seek 25ml @11:26:58.65 Vacuum too high -- waiting up to 40s @11:27:00.27 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:27:00.29 SS.reconfigure SSinhale.with maxSpeed: 53 @11:27:00.34 SS.seek 25ml @11:27:04.87 Vacuum too high -- waiting up to 40s @11:27:06.74 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:27:06.76 SS.reconfigure SSinhale.with maxSpeed: 48 @11:27:06.80 SS.seek 25ml @11:27:13.85 Vacuum too high -- waiting up to 40s @11:27:15.47 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:27:15.48 SS.reconfigure SSinhale.with maxSpeed: 43 @11:27:15.53 SS.seek 25ml @11:27:25.09 Vacuum too high -- waiting up to 40s @11:27:26.68 SS.reconfigure SSinhale.with maxSpeed: 39 @11:27:26.73 SS.seek 25ml @11:27:40.68 Stroke completed -- waiting for pressure to equalize ... @11:27:45.93 Intake.select :close @11:27:46.09 SSV.dial :exhaust @11:27:46.94 Exhaust.select :open @11:27:47.07 SS.reconfigure SSexhale @11:27:47.12 SS.seek 0ml @11:27:56.22 Exhaust.select :close @11:27:56.33 SSV.dial CBV @11:27:56.37 CBV.dial SSV @11:27:57.23 Allowing 130s for 25.0ml of remaining 1450.0ml @11:27:57.25 Intake.select :open @11:27:59.43 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:27:59.45 SS.reconfigure SSinhale.with maxSpeed: 39 @11:27:59.49 SS.seek 25ml @11:28:17.35 delay 5 seconds @11:28:22.38 ATV.dial ASV @11:28:22.54 Vacuum too high -- waiting up to 40s @11:28:23.36 ASV.dial :SPEdelivery @11:28:23.38 AS.seek 0.02ml @11:28:24.17 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:28:24.19 SS.reconfigure SSinhale.with maxSpeed: 35 @11:28:24.23 SS.seek 25ml @11:28:49.53 Stroke completed -- waiting for pressure to equalize ... @11:28:54.65 Intake.select :close @11:28:54.81 SSV.dial :exhaust @11:28:55.66 Exhaust.select :open @11:28:55.78 SS.reconfigure SSexhale @11:28:55.83 SS.seek 0ml @11:29:04.98 Exhaust.select :close @11:29:05.09 SSV.dial CBV @11:29:05.12 CBV.dial SSV @11:29:05.97 Allowing 130s for 25.0ml of remaining 1425.0ml @11:29:05.98 Intake.select :open @11:29:08.17 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:29:08.19 SS.reconfigure SSinhale.with maxSpeed: 35 @11:29:08.23 SS.seek 25ml @11:29:58.69 Stroke completed -- waiting for pressure to equalize ... @11:30:03.82 Intake.select :close @11:30:03.98 SSV.dial :exhaust @11:30:04.81 Exhaust.select :open @11:30:04.93 SS.reconfigure SSexhale @11:30:04.98 SS.seek 0ml @11:30:13.77 Exhaust.select :close @11:30:13.89 SSV.dial CBV @11:30:13.91 CBV.dial SSV @11:30:15.50 Allowing 130s for 25.0ml of remaining 1400.0ml @11:30:15.51 Intake.select :open @11:30:17.69 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:30:17.71 SS.reconfigure SSinhale.with maxSpeed: 35 @11:30:17.76 SS.seek 25ml @11:31:08.42 Stroke completed -- waiting for pressure to equalize ... @11:31:14.88 Intake.select :close @11:31:15.04 SSV.dial :exhaust @11:31:15.89 Exhaust.select :open @11:31:16.01 SS.reconfigure SSexhale @11:31:16.06 SS.seek 0ml @11:31:25.17 Exhaust.select :close @11:31:25.29 SSV.dial CBV @11:31:25.31 CBV.dial SSV @11:31:26.16 Allowing 130s for 25.0ml of remaining 1375.0ml @11:31:26.17 Intake.select :open @11:31:28.35 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:31:28.37 SS.reconfigure SSinhale.with maxSpeed: 35 @11:31:28.42 SS.seek 25ml @11:31:40.75 delay 5 seconds @11:31:45.78 ATV.dial :air2 @11:31:46.83 AS.seek 0.52ml @11:32:04.32 Vacuum too high -- waiting up to 40s @11:32:06.18 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:32:06.20 SS.reconfigure SSinhale.with maxSpeed: 32 @11:32:06.25 SS.seek 25ml @11:32:22.08 Stroke completed -- waiting for pressure to equalize ... @11:32:27.20 Intake.select :close @11:32:27.36 SSV.dial :exhaust @11:32:28.20 Exhaust.select :open @11:32:28.32 SS.reconfigure SSexhale @11:32:28.37 SS.seek 0ml @11:32:37.94 Exhaust.select :close @11:32:38.06 SSV.dial CBV @11:32:38.08 CBV.dial SSV @11:32:38.95 Allowing 130s for 25.0ml of remaining 1350.0ml @11:32:38.96 Intake.select :open @11:32:41.15 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:32:41.17 SS.reconfigure SSinhale.with maxSpeed: 32 @11:32:41.21 SS.seek 25ml @11:33:27.69 delay 5 seconds @11:33:32.72 ATV.dial ASV @11:33:33.70 ASV.dial :wasteR @11:33:34.07 AS.seek 0.3ml @11:33:36.13 Stroke completed -- waiting for pressure to equalize ... @11:33:41.99 Intake.select :close @11:33:42.15 SSV.dial :exhaust @11:33:43.00 Exhaust.select :open @11:33:43.14 SS.reconfigure SSexhale @11:33:43.19 SS.seek 0ml @11:33:52.39 Exhaust.select :close @11:33:52.51 SSV.dial CBV @11:33:52.53 CBV.dial SSV @11:33:53.41 Allowing 130s for 25.0ml of remaining 1325.0ml @11:33:53.43 Intake.select :open @11:33:55.61 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:33:55.63 SS.reconfigure SSinhale.with maxSpeed: 32 @11:33:55.67 SS.seek 25ml @11:34:18.59 delay 20 seconds @11:34:38.63 AS.seek 0.07ml @11:34:45.70 Vacuum too high -- waiting up to 40s @11:34:47.82 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:34:47.84 SS.reconfigure SSinhale.with maxSpeed: 29 @11:34:47.89 SS.seek 25ml @11:34:53.96 Stroke completed -- waiting for pressure to equalize ... @11:34:58.46 Intake.select :close @11:34:58.62 SSV.dial :exhaust @11:34:59.46 Exhaust.select :open @11:34:59.58 SS.reconfigure SSexhale @11:34:59.63 SS.seek 0ml @11:35:08.96 Exhaust.select :close @11:35:09.08 SSV.dial CBV @11:35:09.10 CBV.dial SSV @11:35:09.96 Allowing 130s for 25.0ml of remaining 1300.0ml @11:35:09.97 Intake.select :open @11:35:12.16 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:35:12.18 SS.reconfigure SSinhale.with maxSpeed: 29 @11:35:12.22 SS.seek 25ml @11:35:25.17 delay 20 seconds @11:35:45.20 ATV.dial :waste2 @11:35:45.95 AS.reconfigure ASconfig @11:35:46.00 AS.seek :empty @11:35:50.40 Elution @11:35:50.42 ATV.dial :air1 @11:35:51.52 AS.reconfigure ASslow1 @11:35:51.57 AS.seek 0.049ml @11:36:01.98 delay 3 seconds @11:36:05.01 ATV.dial :ElutionWater @11:36:05.69 AS.seek 0.109ml @11:36:12.94 Stroke completed -- waiting for pressure to equalize ... @11:36:18.03 delay 3 seconds @11:36:19.25 Intake.select :close @11:36:19.41 SSV.dial :exhaust @11:36:20.25 Exhaust.select :open @11:36:20.37 SS.reconfigure SSexhale @11:36:20.42 SS.seek 0ml @11:36:21.05 ATV.dial ASV,via: :air2 @11:36:23.10 ASV.dial :SPEelution @11:36:23.69 AFV.select :carrierSolution @11:36:23.86 AS.reconfigure ASnorm @11:36:23.91 AS.seek 0.759ml @11:36:29.32 Exhaust.select :close @11:36:29.43 SSV.dial CBV @11:36:29.47 CBV.dial SSV @11:36:30.33 Allowing 130s for 25.0ml of remaining 1275.0ml @11:36:30.34 Intake.select :open @11:36:32.52 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:36:32.54 SS.reconfigure SSinhale.with maxSpeed: 29 @11:36:32.59 SS.seek 25ml @11:36:56.95 delay 3 seconds @11:36:59.98 AFV.select :holdingCoil @11:37:00.13 AS.reconfigure ASslow1 @11:37:00.19 AS.seek 0.474ml @11:37:33.43 Stroke completed -- waiting for pressure to equalize ... @11:37:41.10 Intake.select :close @11:37:41.26 SSV.dial :exhaust @11:37:42.11 Exhaust.select :open @11:37:42.23 SS.reconfigure SSexhale @11:37:42.28 SS.seek 0ml @11:37:51.68 Exhaust.select :close @11:37:51.80 SSV.dial CBV @11:37:51.82 CBV.dial SSV @11:37:52.68 Allowing 130s for 25.0ml of remaining 1250.0ml @11:37:52.69 Intake.select :open @11:37:54.88 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:37:54.90 SS.reconfigure SSinhale.with maxSpeed: 29 @11:37:54.94 SS.seek 25ml @11:37:57.80 delay 5 seconds @11:38:02.90 AS.reconfigure ASslow2 @11:38:02.95 AS.seek 0.439ml @11:38:38.49 delay 1:00 @11:38:38.87 Vacuum too high -- waiting up to 40s @11:38:41.27 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:38:41.29 SS.reconfigure SSinhale.with maxSpeed: 26 @11:38:41.34 SS.seek 25ml @11:39:00.30 Stroke completed -- waiting for pressure to equalize ... @11:39:06.81 Intake.select :close @11:39:06.97 SSV.dial :exhaust @11:39:07.82 Exhaust.select :open @11:39:07.94 SS.reconfigure SSexhale @11:39:07.99 SS.seek 0ml @11:39:17.13 Exhaust.select :close @11:39:17.26 SSV.dial CBV @11:39:17.28 CBV.dial SSV @11:39:18.14 Allowing 130s for 25.0ml of remaining 1225.0ml @11:39:18.15 Intake.select :open @11:39:20.34 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:39:20.36 SS.reconfigure SSinhale.with maxSpeed: 26 @11:39:20.40 SS.seek 25ml @11:39:38.53 pushing eluate to holding coil @11:39:38.60 AS.seek 0.279ml @11:40:27.77 Stroke completed -- waiting for pressure to equalize ... @11:40:36.08 Intake.select :close @11:40:36.24 SSV.dial :exhaust @11:40:37.10 Exhaust.select :open @11:40:37.22 SS.reconfigure SSexhale @11:40:37.27 SS.seek 0ml @11:40:46.49 Exhaust.select :close @11:40:46.61 SSV.dial CBV @11:40:46.63 CBV.dial SSV @11:40:47.50 Allowing 130s for 25.0ml of remaining 1200.0ml @11:40:47.51 Intake.select :open @11:40:49.70 Intake @32.0psia, vacuum from 20.0 to 24.0psi @11:40:49.72 SS.reconfigure SSinhale.with maxSpeed: 26 @11:40:49.76 SS.seek 25ml @11:41:32.73 Vacuum too high -- waiting up to 40s @11:41:35.39 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:41:35.41 SS.reconfigure SSinhale.with maxSpeed: 23 @11:41:35.46 SS.seek 25ml @11:42:03.33 Stroke completed -- waiting for pressure to equalize ... @11:42:11.75 Intake.select :close @11:42:11.91 SSV.dial :exhaust @11:42:12.76 Exhaust.select :open @11:42:12.88 SS.reconfigure SSexhale @11:42:12.93 SS.seek 0ml @11:42:19.74 delay 30 seconds @11:42:21.90 Exhaust.select :close @11:42:22.02 SSV.dial CBV @11:42:22.04 CBV.dial SSV @11:42:22.91 Allowing 130s for 25.0ml of remaining 1175.0ml @11:42:22.93 Intake.select :open @11:42:25.12 Intake @32.0psia, vacuum from 20.0 to 24.0psi @11:42:25.14 SS.reconfigure SSinhale.with maxSpeed: 23 @11:42:25.18 SS.seek 25ml @11:42:49.76 ATV.dial :waste,via: :air3 @11:42:51.46 AS.reconfigure ASconfig @11:42:51.51 AS.seek :empty @11:43:07.89 Parking Bleach in LysateSource line and SPEcolumn @11:43:07.90 ATV.dial :air3 @11:43:08.75 AS.reconfigure ASslow1 @11:43:08.80 AS.seek 0.029ml @11:43:15.14 ATV.dial :ElutionWater @11:43:15.76 AS.seek 0.059ml @11:43:22.08 ATV.dial :air3 @11:43:22.70 AS.seek 0.069ml @11:43:24.96 delay 2 seconds @11:43:26.98 ATV.dial :bleach @11:43:27.47 AS.seek 0.769ml @11:43:34.97 Vacuum too high -- waiting up to 40s @11:43:37.89 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:43:37.91 SS.reconfigure SSinhale.with maxSpeed: 21 @11:43:37.95 SS.seek 25ml @11:43:45.37 Stroke completed -- waiting for pressure to equalize ... @11:43:53.74 Intake.select :close @11:43:53.90 SSV.dial :exhaust @11:43:54.76 Exhaust.select :open @11:43:54.88 SS.reconfigure SSexhale @11:43:54.93 SS.seek 0ml @11:44:04.38 Exhaust.select :close @11:44:04.50 SSV.dial CBV @11:44:04.52 CBV.dial SSV @11:44:05.40 Allowing 130s for 25.0ml of remaining 1150.0ml @11:44:05.42 Intake.select :open @11:44:07.60 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:44:07.62 SS.reconfigure SSinhale.with maxSpeed: 21 @11:44:07.67 SS.seek 25ml @11:45:31.13 Stroke completed -- waiting for pressure to equalize ... @11:45:42.00 Intake.select :close @11:45:42.16 SSV.dial :exhaust @11:45:43.02 Exhaust.select :open @11:45:43.14 SS.reconfigure SSexhale @11:45:43.19 SS.seek 0ml @11:45:48.51 delay 3 seconds @11:45:51.54 ATV.dial :air3 @11:45:52.10 delay 3 seconds @11:45:52.63 Exhaust.select :close @11:45:52.75 SSV.dial CBV @11:45:52.77 CBV.dial SSV @11:45:53.66 Allowing 130s for 25.0ml of remaining 1125.0ml @11:45:53.67 Intake.select :open @11:45:55.18 AS.seek 0.794ml @11:45:55.85 Intake @32.0psia, vacuum from 20.0 to 24.0psi @11:45:55.87 SS.reconfigure SSinhale.with maxSpeed: 21 @11:45:55.91 SS.seek 25ml @11:46:00.47 delay 3 seconds @11:46:03.50 ATV.dial :bleach @11:46:04.12 AS.seek 0.779ml @11:46:07.44 delay 3 seconds @11:46:10.47 ATV.dial :AIV @11:46:11.72 AS.reconfigure ASconfig @11:46:11.78 AS.seek 0.015ml @11:46:41.10 Vacuum too high -- waiting up to 40s @11:46:44.22 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:46:44.94 SS.reconfigure SSinhale.with maxSpeed: 19 @11:46:45.02 SS.seek 25ml @11:46:55.99 ATV.dial :waste @11:46:57.47 AS.seek :empty @11:46:58.69 ATV.dial :air3 @11:46:59.37 AS.reconfigure ASslow1 @11:46:59.42 AS.seek 0.009ml @11:47:01.76 delay 2 seconds @11:47:03.78 ATV.dial :bleach @11:47:04.25 AS.seek 0.579ml @11:47:27.85 Stroke completed -- waiting for pressure to equalize ... @11:47:39.32 Intake.select :close @11:47:39.48 SSV.dial :exhaust @11:47:40.33 Exhaust.select :open @11:47:40.45 SS.reconfigure SSexhale @11:47:40.50 SS.seek 0ml @11:47:49.68 Exhaust.select :close @11:47:49.80 SSV.dial CBV @11:47:49.82 CBV.dial SSV @11:47:50.68 Allowing 130s for 25.0ml of remaining 1100.0ml @11:47:50.69 Intake.select :open @11:47:52.88 Intake @32.0psia, vacuum from 20.0 to 24.0psi @11:47:52.90 SS.reconfigure SSinhale.with maxSpeed: 19 @11:47:52.94 SS.seek 25ml @11:48:44.16 Vacuum too high -- waiting up to 40s @11:48:47.54 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:48:47.56 SS.reconfigure SSinhale.with maxSpeed: 17 @11:48:47.61 SS.seek 25ml @11:48:59.13 delay 3 seconds @11:49:02.16 ATV.dial :air3 @11:49:02.71 delay 3 seconds @11:49:05.80 AS.seek 0.604ml @11:49:11.08 delay 3 seconds @11:49:14.10 ATV.dial :bleach @11:49:14.72 AS.seek 0.589ml @11:49:17.99 delay 3 seconds @11:49:21.01 ATV.dial :ASV @11:49:22.77 ASV.dial :wasteR @11:49:23.36 AS.seek 0ml @11:49:34.10 Stroke completed -- waiting for pressure to equalize ... @11:49:46.87 Intake.select :close @11:49:47.03 SSV.dial :exhaust @11:49:47.87 Exhaust.select :open @11:49:47.99 SS.reconfigure SSexhale @11:49:48.04 SS.seek 0ml @11:49:57.32 Exhaust.select :close @11:49:57.45 SSV.dial CBV @11:49:57.47 CBV.dial SSV @11:49:58.34 Allowing 130s for 25.0ml of remaining 1075.0ml @11:49:58.35 Intake.select :open @11:50:00.54 Intake @32.1psia, vacuum from 20.0 to 24.0psi @11:50:00.56 SS.reconfigure SSinhale.with maxSpeed: 17 @11:50:00.60 SS.seek 25ml @11:51:03.13 Vacuum too high -- waiting up to 40s @11:51:07.26 Intake @31.9psia, vacuum from 20.0 to 24.0psi @11:51:07.28 SS.reconfigure SSinhale.with maxSpeed: 15 @11:51:07.32 SS.seek 25ml @11:51:22.12 delay 1:00 @11:51:53.73 Stroke completed -- waiting for pressure to equalize ... @11:52:08.44 SS.reconfigure SSconfig @11:52:08.50 SS.hold @11:52:08.63 Intake.select :close @11:52:08.79 ShallowSampler::Clogged in WCR -- progress too slow after 450.0ml @11:52:08.81 SSV.dial :exhaust @11:52:09.66 Exhaust.select :open @11:52:09.78 SS.reconfigure SSexhale @11:52:09.83 SS.seek 0ml @11:52:19.15 Exhaust.select :close @11:52:19.26 Sampled 450.0ml @11:52:19.28 Drying puck before fixing for 4:00 @11:52:19.29 CBV.dial :waste2b @11:52:20.13 delay 1:00 @11:52:22.15 AFV.select :carrierSolution @11:52:22.25 delay 2 seconds @11:52:24.28 AS.reconfigure ASconfig @11:52:24.34 AS.seek 1ml @11:53:20.16 CSV.dial :air @11:53:20.64 CS.reconfigure CSfast @11:53:20.69 CS.seek 10ml @11:53:22.15 delay 1 second @11:53:23.17 AFV.select :holdingCoil @11:53:23.27 delay 1 second @11:53:24.29 ATV.dial ASV @11:53:24.30 ASV.dial :wasteR @11:53:24.32 AS.reconfigure ASslow1 @11:53:24.36 AS.seek 0ml @11:53:45.78 Pumping wcr puck from tube 3 up to 55psi... @11:53:45.80 CBV.dial SSV @11:53:47.21 CBV.dial between CSV and waste2b @11:53:48.28 CSV.dial between air and CTV @11:53:48.63 CS.seek 7.226ml @11:53:56.44 CTV.dial CSV,avoiding: :air @11:53:56.46 CSV.dial CTV @11:53:57.36 CS.reconfigure CSpressurize @11:53:57.41 CS.seek 0ml @11:54:15.14 CBV.dial :waste2b @11:54:15.49 CS.reconfigure CSconfig @11:54:15.54 CS.seek :empty @11:54:18.06 delay 1:00 @11:55:18.09 CTV.dial :air,avoiding: :intake @11:55:19.56 Fix #1 of 2 (with rnal) for 2:00 @11:55:19.58 CSV.dial CSR @11:55:20.12 Pulling 2ml of CSR.rnal @11:55:20.14 CSR.select :rnal @11:55:20.26 CS.seek 2ml @11:55:26.34 delay 5 seconds @11:55:31.37 CSR.select :air @11:55:31.50 CS.reconfigure CSfast @11:55:31.55 CS.seek 10ml @11:55:51.81 Pumping wcr puck from tube 3 up to 55psi... @11:55:52.53 CBV.dial SSV @11:55:53.96 CBV.dial between CSV and waste2b @11:55:55.05 CSV.dial between air and CTV @11:55:55.73 CS.seek 7.2ml @11:56:03.41 CTV.dial CSV,avoiding: :air @11:56:03.43 CSV.dial CTV @11:56:04.98 CS.reconfigure CSpressurize @11:56:05.03 CS.seek 0ml @11:56:23.36 delay 2:00 @11:56:45.76 delay 20 seconds @11:57:05.79 ATV.dial :air3 @11:57:07.27 AS.seek 0.59ml @11:58:23.39 CBV.dial :waste2b @11:58:23.76 CS.reconfigure CSconfig @11:58:23.81 CS.seek :empty @11:58:25.31 delay 1:00 @11:59:06.20 delay 3 seconds @11:59:09.23 ATV.dial :ASV @11:59:10.61 ASV.dial :wasteR @11:59:10.69 AS.seek 0.09ml @11:59:25.34 CTV.dial :air,avoiding: :intake @11:59:26.82 Fix #2 of 2 (with rnal) for 2:00 @11:59:26.83 CSV.dial CSR @11:59:27.37 Pulling 2ml of CSR.rnal @11:59:27.39 CSR.select :rnal @11:59:27.51 CS.seek 2ml @11:59:33.63 delay 5 seconds @11:59:38.66 CSR.select :air @11:59:38.78 CS.reconfigure CSfast @11:59:38.83 CS.seek 10ml @11:59:58.88 Pumping wcr puck from tube 3 up to 55psi... @11:59:58.90 CBV.dial SSV @12:00:00.28 CBV.dial between CSV and waste2b @12:00:01.35 CSV.dial between air and CTV @12:00:02.03 CS.seek 7.135ml @12:00:09.98 CTV.dial CSV,avoiding: :air @12:00:10.00 CSV.dial CTV @12:00:11.56 CS.reconfigure CSpressurize @12:00:11.61 CS.seek 0ml @12:00:29.55 delay 2:00 @12:00:51.55 delay 30 seconds @12:01:21.58 ATV.dial :extSrc @12:01:22.33 delay 3 seconds @12:01:25.36 ATV.dial :ElutionWater @12:01:26.44 AS.seek 0.12ml @12:01:32.75 ATV.dial :air3 @12:01:33.35 AS.seek 0.13ml @12:01:35.62 delay 2 seconds @12:01:37.64 ATV.dial :bleach @12:01:38.11 AS.seek 0.23ml @12:01:58.48 delay 3 seconds @12:02:01.51 ATV.dial :air3 @12:02:02.05 delay 3 seconds @12:02:05.14 AS.seek 0.255ml @12:02:10.44 delay 3 seconds @12:02:13.46 ATV.dial :bleach @12:02:14.07 AS.seek 0.24ml @12:02:17.34 delay 3 seconds @12:02:20.36 ATV.dial :ASV @12:02:21.38 ASV.dial :SPEdelivery @12:02:22.45 AFV.select :carrierSolution @12:02:22.56 delay 2 seconds @12:02:24.59 AS.reconfigure ASconfig @12:02:24.64 AS.seek 0.5ml @12:02:29.58 CBV.dial :waste2b @12:02:29.96 CS.reconfigure CSconfig @12:02:30.02 CS.seek :empty @12:02:31.72 delay 1:00 @12:02:39.88 delay 1 second @12:02:40.90 AFV.select :holdingCoil @12:02:41.05 AS.reconfigure ASslow1 @12:02:41.11 AS.seek 0.17ml @12:03:31.75 CTV.dial :air,avoiding: :intake @12:03:33.29 Storing wcr puck in Collection Clamp to tube 2 @12:03:33.48 SC.reconfigure SCtight @12:03:33.54 SC.seek 2 @12:03:35.93 SC.reconfigure SCloose @12:03:36.16 Elbow.reconfigure TightElbow @12:03:36.21 Elbow.seek 2 @12:03:38.12 Forearm.reconfigure TightForearm @12:03:38.17 Forearm.seek 2 + 700 counts @12:03:39.20 SE.reconfigure SEpick @12:03:39.28 SE.seek beamBreak @12:03:40.93 SE.configure SEconfig @12:03:41.11 Elbow.reconfigure LimpElbow @12:03:41.16 Forearm.reconfigure LooseForearm @12:03:41.21 Forearm.seek 2 @12:03:41.53 Elbow.hold @12:03:41.61 Elbow.reconfigure TightElbow @12:03:41.72 SE.seek 16.08 Pucks below Top Plate @12:03:42.25 Forearm.force -20 @12:03:42.30 Forearm.seek 2 + 341 counts @12:03:42.63 Forearm.reconfigure TightForearm @12:03:42.68 Forearm.seek 2 + 700 counts @12:03:43.00 SE.seek 17.08 Pucks below Top Plate @12:03:43.78 Forearm.reconfigure LooseForearm @12:03:43.83 Forearm.seek :retract @12:03:44.76 Elbow.seek CC @12:03:46.49 Elbow.reconfigure LooseElbow @12:03:46.59 Forearm.seek CC @12:03:47.64 Hand.close @12:03:47.78 SPE.hold 2:46:40,95C @12:03:47.84 delay 3:00 @12:03:48.43 CC.seek :open @12:03:52.97 Forearm.seek :retract @12:03:54.05 Elbow.reconfigure TightElbow @12:03:54.10 Elbow.seek 2 @12:03:56.19 Elbow.reconfigure LimpElbow @12:03:56.93 Forearm.seek 2 @12:03:57.99 Elbow.hold @12:03:58.07 Elbow.reconfigure TightElbow @12:03:58.13 Hand.open @12:03:59.08 Forearm.force -20 @12:03:59.13 Forearm.seek 2 + 339 counts @12:03:59.45 Forearm.reconfigure TightForearm @12:03:59.51 Forearm.seek 2 + 700 counts @12:03:59.91 SE.seek :down @12:04:01.07 SE.coast @12:04:01.10 Forearm.reconfigure LooseForearm @12:04:01.19 Forearm.seek :retract @12:04:02.10 SC.coast @12:04:02.15 Starting finalPurge @12:04:02.16 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:04:02.24 Hand.open @12:04:02.58 Elbow.seek FlushPuck::Garage @12:04:04.66 Elbow.reconfigure LooseElbow @12:04:04.77 Forearm.seek :garage @12:04:05.78 Hand.close @12:04:06.70 Forearm.seek :retract @12:04:07.71 Elbow.reconfigure TightElbow @12:04:07.76 Elbow.seek CC @12:04:10.78 Elbow.reconfigure LooseElbow @12:04:10.88 Forearm.reconfigure TightForearm @12:04:10.93 Forearm.seek CC @12:04:12.29 CC.seek :closed @12:04:16.59 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.44 @12:04:16.68 Hand.open @12:04:17.50 Forearm.reconfigure LooseForearm @12:04:17.55 Forearm.seek :retract @12:04:18.54 Flushing the Collection Valve::Manifold @12:04:18.69 CSV.dial CSR @12:04:19.24 Pulling 5ml of CSR.flush @12:04:19.25 CSR.select :flush @12:04:19.38 CS.seek 5ml @12:04:33.19 delay 5 seconds @12:04:38.22 CSR.select :air @12:04:38.39 CS.reconfigure CSfast @12:04:38.43 CS.seek 7.5ml @12:04:45.69 CSV.dial :waste @12:04:47.19 CS.reconfigure CSconfig @12:04:47.24 CS.seek 0ml @12:05:07.41 Flushing lower manifold's waste1 port @12:05:08.28 CSV.dial :flush @12:05:08.80 CS.reconfigure CSfast @12:05:08.84 CS.seek 5ml @12:05:22.45 delay 5 seconds @12:05:27.48 CSV.dial :air @12:05:28.07 CS.seek 7ml @12:05:34.16 CSV.dial CTV @12:05:34.19
CTV.dial CSV @12:05:35.82 CBV.dial :waste1 @12:05:36.71 CS.seek 0ml @12:05:55.10 Flushing lower manifold's waste2b port @12:05:55.24 CSV.dial :flush @12:05:56.11 CS.seek 5ml @12:06:09.82 delay 5 seconds @12:06:14.85 CSV.dial :air @12:06:15.45 CS.seek 7ml @12:06:21.45 CSV.dial CTV @12:06:21.48
CTV.dial CSV @12:06:21.98 CBV.dial :waste2b @12:06:22.86 CS.seek 0ml @12:06:41.38 Flushing upper manifold @12:06:41.52 CSV.dial :flush @12:06:42.36 CS.seek 5ml @12:06:47.89 AFV.select :carrierSolution @12:06:47.99 delay 2 seconds @12:06:50.02 AS.reconfigure ASconfig @12:06:50.07 AS.seek 1ml @12:06:55.85
delay 5 seconds @12:07:00.88 CSV.dial :air @12:07:01.47 CS.seek 7ml @12:07:07.48 CSV.dial CBV @12:07:07.51
CBV.dial CSV @12:07:08.76 CTV.dial :air @12:07:10.30 CS.seek 0ml @12:07:28.70 delay 7 seconds @12:07:35.74 CS.seek 3ml @12:07:38.07 delay 1 second @12:07:39.09 AFV.select :holdingCoil @12:07:39.19 delay 1 second @12:07:40.21 ASV.dial :wasteD @12:07:41.37 AS.seek 0ml @12:07:44.20
CSV.dial :waste @12:07:44.72 CS.seek 0ml @12:07:53.42 CSV.dial :flush @12:07:53.93 CS.seek 3ml @12:08:02.26 delay 5 seconds @12:08:07.29 CSV.dial :air @12:08:07.88 CS.seek 5ml @12:08:13.86 CSV.dial :air @12:08:13.88 CS.seek 0ml @12:08:27.64 delay 7 seconds @12:08:34.68 CS.seek 3ml @12:08:39.15 Priming Eluate @12:08:39.16 AFV.select :carrierSolution @12:08:39.27 delay 2 seconds @12:08:41.31 AS.seek 0.5ml @12:08:43.11
CSV.dial :waste @12:08:43.96 CS.seek 0ml @12:08:52.36 Disinfecting external Intake with mfbkill @12:08:52.50 CSV.dial CSR @12:08:54.02 Pulling 8ml of CSR.mfbkill @12:08:54.03 CSR.select :mfbkill @12:08:54.16 CS.seek 8ml @12:09:10.38 delay 1 second @12:09:11.40 AFV.select :holdingCoil @12:09:11.50 ATV.dial ASV @12:09:11.51 ASV.dial :SPErecovery @12:09:12.11 Swish #1 of 2 @12:09:12.19 AS.reconfigure ASslow0 @12:09:12.24 AS.seek 0.6ml @12:09:14.79
delay 5 seconds @12:09:19.82 CSR.select :air @12:09:19.94 CS.seek 10ml @12:09:22.75 AS.seek 0.5ml @12:09:25.92 CSV.dial CBV @12:09:25.95
CBV.dial CSV @12:09:27.12 CTV.dial :intake @12:09:27.98 Intake.select :open @12:09:28.10 CS.seek 2ml @12:09:33.88 Swish #2 of 2 @12:09:33.96 AS.seek 0.6ml @12:09:44.43 AS.seek 0.5ml @12:09:49.95
Intake.select :close @12:09:50.06 CTV.dial :air @12:09:50.92 CS.reconfigure CSconfig @12:09:50.97 CS.seek 0ml @12:09:54.83 delay 3 seconds @12:09:57.11
Flushing lower manifold's waste1 port @12:09:57.12 CSV.dial CSR @12:09:57.91 AS.reconfigure ASslow1 @12:09:57.96 AS.seek 0.684ml @12:09:58.35
Pulling 4ml of CSR.mfbkill @12:09:58.36 CSR.select :mfbkill @12:09:58.48 CS.seek 4ml @12:10:09.86 delay 5 seconds @12:10:14.89 CSR.select :air @12:10:15.02 CS.reconfigure CSfast @12:10:15.06 CS.seek 10ml @12:10:30.73 CSV.dial CTV @12:10:30.76
CTV.dial CSV @12:10:32.34 CBV.dial :waste1 @12:10:32.85 CS.seek 0ml @12:10:35.26 delay 3 seconds @12:10:38.29 Eluate primed @12:10:38.30 ATV.dial :waste2 @12:10:38.91 AS.reconfigure ASnorm @12:10:38.96 AS.seek 0ml @12:10:58.17
Flushing lower manifold's waste2b port @12:10:58.18 CSV.dial CSR @12:10:58.77 Pulling 4ml of CSR.mfbkill @12:10:58.78 CSR.select :mfbkill @12:10:58.90 CS.reconfigure CSconfig @12:10:58.95 CS.seek 4ml @12:11:10.11 delay 5 seconds @12:11:13.76 SPE.coast @12:11:13.85 ASV.dial :SPErecovery @12:11:13.87 Setting up PCR for OM -- writing /var/log/esp/OM20may2512h400ml.pcr @12:11:13.89 Building PCR Slug from ASV with MM3 mix, PP5 primer @12:11:13.91 Priming with carrier ATV.dial ARV @12:11:14.87 ARV.dial :carrier @12:11:15.13
CSR.select :air @12:11:15.31 AS.seek 0.5ml @12:11:15.37
CS.reconfigure CSfast @12:11:15.42 CS.seek 10ml @12:11:30.67 CSV.dial CTV @12:11:30.70
CTV.dial CSV @12:11:31.20 CBV.dial :waste2b @12:11:32.06 CS.seek 0ml @12:11:40.93 AFV.select :carrierSolution @12:11:41.04 delay 2 seconds @12:11:43.07 AS.reconfigure ASconfig @12:11:43.12 AS.seek 1ml @12:11:56.80
Killing upper manifold @12:11:56.81 CSV.dial CSR @12:11:57.37 Pulling 4ml of CSR.mfbkill @12:11:57.38 CSR.select :mfbkill @12:11:57.51 CS.reconfigure CSconfig @12:11:57.56 CS.seek 4ml @12:12:08.73 delay 5 seconds @12:12:12.15 delay 1 second @12:12:13.17 AFV.select :holdingCoil @12:12:13.27 delay 1 second @12:12:13.75
CSR.select :air @12:12:13.87 CS.reconfigure CSfast @12:12:13.92 CS.seek 10ml @12:12:14.30 ATV.dial :waste @12:12:15.74 AS.reconfigure ASnorm @12:12:15.80 AS.seek :empty @12:12:29.20 CSV.dial CBV @12:12:29.23
CBV.dial CSV @12:12:30.41 CTV.dial :air @12:12:31.87 CS.seek 0ml @12:12:56.49 delay 7 seconds @12:13:03.53 CS.seek 3ml @12:13:06.55 AFV.select :carrierSolution @12:13:06.66 delay 2 seconds @12:13:08.69 AS.reconfigure ASconfig @12:13:09.48 AS.seek 0.5ml @12:13:11.61
CSV.dial :waste @12:13:12.09 CS.seek 0ml @12:13:20.24 Flushing upper manifold @12:13:20.38 CSV.dial :flush @12:13:20.89 CS.seek 5ml @12:13:33.71 delay 5 seconds @12:13:38.55 delay 1 second @12:13:38.73
CSV.dial :air @12:13:39.30 CS.seek 7ml @12:13:39.57 AFV.select :holdingCoil @12:13:39.68 Building Slug @12:13:39.69 ATV.dial :ARV @12:13:41.27 delay 2 seconds @12:13:43.30 ARV.dial :air1 @12:13:43.68 delay 2 seconds @12:13:45.14 CSV.dial CBV @12:13:45.17
CBV.dial CSV @12:13:45.77 AS.reconfigure ASslow2 @12:13:45.83 AS.seek 0.506ml @12:13:46.30
CTV.dial :air @12:13:46.32 CS.seek 0ml @12:13:52.01 delay 2 seconds @12:13:54.04 ARV.dial :MM3 @12:13:55.07 delay 2 seconds @12:13:57.10 Adding MM3 @12:13:57.19 AS.seek 0.524ml @12:14:03.89
delay 7 seconds @12:14:10.93 CS.seek 3ml @12:14:15.49 delay 25 seconds @12:14:18.96
CSV.dial :waste @12:14:19.47 CS.seek 0ml @12:14:27.74 CSV.dial :flush @12:14:28.23 CS.seek 3ml @12:14:36.49 delay 5 seconds @12:14:40.52 ARV.dial :PP5 @12:14:40.90 Adding PP5 @12:14:40.92 delay 2 seconds @12:14:41.51
CSV.dial :air @12:14:42.07 CS.seek 5ml @12:14:43.01 AS.seek 0.53ml @12:14:47.83
CSV.dial :air @12:14:47.85 CS.seek 0ml @12:14:49.30 delay 10 seconds @12:14:59.33 ARV.dial :air3 @12:14:59.71 adding ARV air @12:14:59.73 delay 2 seconds @12:15:00.74
delay 7 seconds @12:15:01.82 AS.seek 0.536ml @12:15:07.77
CS.seek 3ml @12:15:08.09 delay 5 seconds @12:15:13.12 ARV.dial :carrier @12:15:14.35 delay 7 seconds @12:15:15.86
CSV.dial :waste @12:15:16.67 CS.seek 0ml @12:15:21.44 AS.seek 0.594ml @12:15:24.77
Flushing lower manifold's waste1 port @12:15:24.91 CSV.dial :flush @12:15:25.42 CS.seek 5ml @12:15:38.21 delay 5 seconds @12:15:43.24 CSV.dial :air @12:15:43.92 CS.seek 7ml @12:15:49.68 CSV.dial CTV @12:15:49.71
CTV.dial CSV @12:15:51.22 CBV.dial :waste1 @12:15:51.73 CS.seek 0ml @12:16:09.33 Flushing lower manifold's waste2b port @12:16:09.47 CSV.dial :flush @12:16:10.28 CS.seek 5ml @12:16:19.99 delay 5 seconds @12:16:23.27
delay 5 seconds @12:16:25.02 ATV.dial :ASV @12:16:26.17 delay 2 seconds @12:16:28.27 AS.seek 0.6ml @12:16:28.32
CSV.dial :air @12:16:28.90 CS.seek 7.001ml @12:16:34.65 delay 4 seconds @12:16:34.70 CSV.dial CTV @12:16:34.72
CTV.dial CSV @12:16:35.21 CBV.dial :waste2b @12:16:36.06 CS.seek 0ml @12:16:38.69 ATV.dial ARV @12:16:39.23 delay 2 seconds @12:16:41.26 ARV.dial :carrier @12:16:41.27 delay 2 seconds @12:16:43.36 AS.seek 0.65ml @12:16:53.62
Disinfecting external Intake with kill @12:16:53.76 CSV.dial CSR @12:16:54.31 Pulling 8ml of CSR.kill @12:16:54.32 CSR.select :kill @12:16:54.44 CS.seek 8ml @12:17:14.40 delay 5 seconds @12:17:19.43 CSR.select :air @12:17:19.55 CS.seek 10ml @12:17:25.32 CSV.dial CBV @12:17:25.34
CBV.dial CSV @12:17:26.52 CTV.dial :intake @12:17:27.32 Intake.select :open @12:17:27.44 CS.seek 2ml @12:17:34.06 delay 4 seconds @12:17:38.09 ATV.dial PCR @12:17:38.70 ASV.dial :waste1 @12:17:39.48 ARV.dial :air1 @12:17:39.87 Positioning Slug by 0.324ml @12:17:39.96 AS.seek 0.326ml @12:17:48.43
Intake.select :close @12:17:48.54 CTV.dial :air @12:17:49.37 CS.reconfigure CSconfig @12:17:49.42 CS.seek 0ml @12:17:55.55 CSV.dial CSR @12:17:56.72 Pulling 5ml of CSR.flush @12:17:56.73 CSR.select :flush @12:17:56.86 CS.seek 5ml @12:18:10.55 delay 5 seconds @12:18:15.58 CSR.select :air @12:18:15.71 CS.seek 10ml @12:18:29.46 CSV.dial CBV @12:18:30.58 CS.seek 0ml @12:18:57.24 Garaging FlushPuck in Collection Clamp @12:18:57.32 Elbow.reconfigure TightElbow @12:18:57.37 Elbow.seek CC @12:18:57.67 Elbow.reconfigure LooseElbow @12:18:57.77 Forearm.seek CC @12:18:58.77 Hand.close @12:18:59.46 CC.seek :open @12:19:03.84 Forearm.seek :retract @12:19:04.83 Elbow.reconfigure TightElbow @12:19:04.88 Elbow.seek :garage @12:19:08.08 Elbow.reconfigure LooseElbow @12:19:08.19 Forearm.reconfigure TightForearm @12:19:08.24 Forearm.seek :garage @12:19:09.39 Hand.open @12:19:10.15 Forearm.reconfigure LooseForearm @12:19:10.20 Forearm.seek :retract @12:19:11.19 Starting initialPurge @12:19:11.20 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:19:11.28 Hand.open @12:19:11.65 Elbow.reconfigure TightElbow @12:19:11.70 Elbow.seek FlushPuck::Garage @12:19:12.69 Elbow.reconfigure LooseElbow @12:19:12.79 Forearm.seek :garage @12:19:13.77 Hand.close @12:19:14.48 Forearm.seek :retract @12:19:15.49 Elbow.reconfigure TightElbow @12:19:15.54 Elbow.seek CC @12:19:18.51 Elbow.reconfigure LooseElbow @12:19:18.61 Forearm.reconfigure TightForearm @12:19:18.66 Forearm.seek CC @12:19:20.01 CC.seek :closed @12:19:24.26 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.10 @12:19:24.35 Hand.open @12:19:25.20 Forearm.reconfigure LooseForearm @12:19:25.25 Forearm.seek :retract @12:19:26.27 Flushing the Collection Valve::Manifold @12:19:26.41 CSV.dial CSR @12:19:27.59 Pulling 5ml of CSR.flush @12:19:27.60 CSR.select :flush @12:19:27.73 CS.seek 5ml @12:19:41.49 delay 5 seconds @12:19:46.52 CSR.select :air @12:19:46.69 CS.reconfigure CSfast @12:19:46.73 CS.seek 7.5ml @12:19:53.62 CSV.dial :waste @12:19:55.03 CS.reconfigure CSconfig @12:19:55.08 CS.seek 0ml @12:20:15.40 Purging external Intake @12:20:15.54 CSV.dial :flush @12:20:16.03 CS.reconfigure CSfast @12:20:16.08 CS.seek 10ml @12:20:40.62 delay 5 seconds @12:20:45.65 CSV.dial CBV @12:20:45.69
CBV.dial CSV @12:20:46.51 CTV.dial :intake @12:20:47.34 Intake.select :open @12:20:47.46 CS.seek 0ml @12:21:13.71 Intake.select :close @12:21:13.82 CTV.dial :air @12:21:14.64 Flushing upper manifold @12:21:14.77 CSV.dial :flush @12:21:15.57 CS.seek 5ml @12:21:28.28 delay 5 seconds @12:21:33.31 CSV.dial :air @12:21:33.86 CS.seek 7ml @12:21:39.50 CSV.dial CBV @12:21:39.53
CBV.dial CSV @12:21:40.65 CTV.dial :air @12:21:40.67 CS.seek 0ml @12:21:58.12 delay 7 seconds @12:22:05.16 CS.seek 3ml @12:22:13.22 CSV.dial :waste @12:22:13.73 CS.seek 0ml @12:22:22.40 CSV.dial :flush @12:22:22.88 CS.seek 3ml @12:22:30.94 delay 5 seconds @12:22:35.97 CSV.dial :air @12:22:36.52 CS.seek 5ml @12:22:42.40 CSV.dial :air @12:22:42.42 CS.seek 0ml @12:22:55.15 delay 7 seconds @12:23:02.19 CS.seek 3ml @12:23:05.97 delay 5 seconds @12:23:10.28
CSV.dial :waste @12:23:11.09 ATV.dial ARV @12:23:11.85 ASV.dial :PCRlock @12:23:11.93
CS.seek 0ml @12:23:12.23 delay 5 seconds @12:23:17.26 Running OM Profile60C350 thermocycling @12:23:17.27 PCR.program Profile60C350 @12:23:17.39 Profile60C350 should complete in < 2:02:45 @12:23:17.40 PCR.run 2:38:26.25 @12:23:20.00
Garaging FlushPuck in Collection Clamp @12:23:20.08 Elbow.reconfigure TightElbow @12:23:20.13 Elbow.seek CC @12:23:20.40 Elbow.reconfigure LooseElbow @12:23:20.50 Forearm.seek CC @12:23:21.47 Hand.close @12:23:22.22 CC.seek :open @12:23:26.60 Forearm.seek :retract @12:23:27.61 Elbow.reconfigure TightElbow @12:23:27.66 Elbow.seek :garage @12:23:30.88 Elbow.reconfigure LooseElbow @12:23:30.99 Forearm.reconfigure TightForearm @12:23:31.04 Forearm.seek :garage @12:23:32.20 Hand.open @12:23:33.04 Forearm.reconfigure LooseForearm @12:23:33.09 Forearm.seek :retract @12:23:34.11 Completed [stdphase] begun 10:02:09 @12:23:34.12 Preparing to sleep until Tue May 26 10:02:09 +0200 2020 @12:23:34.18 deferred until MFBPCR finish @12:23:34.20
Waiting for MFBPCR til 10:02CEST26-May-20 @12:23:34.22 Continuing at 10:02CEST26-May-20 @12:23:34.29 GoodNews.email "Then, in tube 3: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:150\n{1 => 22, 2 => 18, 3 => 4}\nCan@12:23:34, 9.0C, 50% humidity, 14.4psia, 12.871V, 0.922A, 0.922A avg, 11.87W",Subject: "Waiting until 10:02CEST26-May-20" @14:00:03.06 PCR.off @14:00:04.33 PCR for OM completed -- closed /var/log/esp/OM20may2512h400ml.pcr @14:00:04.41 Cleaning PCR @14:00:04.42 AFV.select :carrierSolution @14:00:04.53 delay 2 seconds @14:00:06.56 AS.reconfigure ASconfig @14:00:06.61 AS.seek 0.5ml @14:00:16.90 delay 1 second @14:00:17.92 AFV.select :holdingCoil @14:00:18.02 ASV.dial :waste1 @14:00:18.40 ATV.dial :air3 @14:00:19.63 AS.reconfigure ASslow1 @14:00:19.68 AS.seek 0.51ml @14:00:22.02 delay 2 seconds @14:00:24.04 ATV.dial :bleach @14:00:24.65 AS.seek 0.81ml @14:01:25.23 delay 3 seconds @14:01:28.26 ATV.dial :air3 @14:01:28.79 delay 3 seconds @14:01:31.88 AS.seek 0.835ml @14:01:37.18 delay 3 seconds @14:01:40.21 ATV.dial :bleach @14:01:40.82 AS.seek 0.82ml @14:01:44.09 delay 3 seconds @14:01:47.12 ARV.dial :waste @14:01:47.70 delay 3 seconds @14:01:50.73 ATV.dial :ARV @14:01:51.60 AS.reconfigure ASconfig @14:01:51.65 AS.seek 0.47ml @14:02:12.15 AFV.select :carrierSolution @14:02:12.26 delay 2 seconds @14:02:14.30 AS.seek 1ml @14:02:45.08 delay 1 second @14:02:46.10 AFV.select :holdingCoil @14:02:46.20 delay 1 second @14:02:47.23 AS.reconfigure ASfast @14:02:47.28 AS.seek 0ml @14:03:14.62 AFV.select :carrierSolution @14:03:14.73 delay 2 seconds @14:03:16.76 AS.reconfigure ASconfig @14:03:16.81 AS.seek 1ml @14:04:14.61 delay 1 second @14:04:15.63 AFV.select :holdingCoil @14:04:15.73 delay 1 second @14:04:16.76 AS.reconfigure ASfast @14:04:16.81 AS.seek 0ml @14:04:44.16 AFV.select :carrierSolution @14:04:44.27 delay 2 seconds @14:04:46.30 AS.reconfigure ASconfig @14:04:46.35 AS.seek 1ml @14:05:44.13 delay 1 second @14:05:45.15 AFV.select :holdingCoil @14:05:45.25 delay 1 second @14:05:46.28 AS.reconfigure ASfast @14:05:46.33 AS.seek 0ml @14:06:13.67 ARV.dial between carrier and air1 @14:06:14.16 ATV.dial :air3 @14:06:15.40 AS.reconfigure ASslow1 @14:06:15.45 AS.seek 0.01ml @14:06:17.80 delay 2 seconds @14:06:19.82 ATV.dial :bleach @14:06:20.43 AS.seek 0.81ml @14:09:01.57 delay 3 seconds @14:09:04.60 ATV.dial :air3 @14:09:05.14 delay 3 seconds @14:09:08.23 AS.seek 0.835ml @14:09:13.50 delay 3 seconds @14:09:17.27 ATV.dial :bleach @14:09:17.87 AS.seek 0.82ml @14:09:21.14 delay 3 seconds @14:09:24.17 ATV.dial PCR @14:09:24.57 AS.reconfigure ASconfig @14:09:24.62 AS.seek :empty @14:10:12.10 PCR.program :bleachProfile @14:10:12.16 PCR program should complete in < 2:45 @14:10:12.17 PCR.run 10:00 @14:13:26.11 PCR.off @14:13:26.14 AFV.select :carrierSolution @14:13:26.25 delay 2 seconds @14:13:28.29 AS.seek 1ml @14:14:26.08 delay 1 second @14:14:27.10 AFV.select :holdingCoil @14:14:27.20 delay 1 second @14:14:28.23 AS.reconfigure ASfast @14:14:28.28 AS.seek 0ml @14:14:55.63 delay 3 seconds @14:14:58.66 AFV.select :carrierSolution @14:14:58.76 delay 2 seconds @14:15:00.79 AS.reconfigure ASconfig @14:15:00.85 AS.seek 1ml @14:15:58.65 delay 1 second @14:15:59.67 AFV.select :holdingCoil @14:15:59.77 delay 1 second @14:16:00.80 AS.reconfigure ASfast @14:16:00.85 AS.seek 0ml @14:16:28.20 delay 3 seconds @14:16:31.23 AFV.select :carrierSolution @14:16:31.33 delay 2 seconds @14:16:33.36 AS.reconfigure ASconfig @14:16:33.42 AS.seek 1ml @14:17:31.16 delay 1 second @14:17:32.18 AFV.select :holdingCoil @14:17:32.28 delay 1 second @14:17:33.31 AS.reconfigure ASfast @14:17:33.36 AS.seek 0ml @14:18:00.68 delay 3 seconds @14:18:03.71 AFV.select :carrierSolution @14:18:03.81 delay 2 seconds @14:18:05.84 AS.reconfigure ASconfig @14:18:05.90 AS.seek 1ml @14:19:03.68 delay 1 second @14:19:04.70 AFV.select :holdingCoil @14:19:04.80 delay 1 second @14:19:05.83 AS.reconfigure ASfast @14:19:05.88 AS.seek 0ml @14:19:33.31 delay 3 seconds @14:19:36.34 AFV.select :carrierSolution @14:19:36.44 delay 2 seconds @14:19:38.47 AS.reconfigure ASconfig @14:19:38.52 AS.seek 1ml @14:20:36.29 delay 1 second @14:20:37.31 AFV.select :holdingCoil @14:20:37.41 delay 1 second @14:20:38.44 AS.reconfigure ASnorm @14:20:38.49 AS.seek 0ml @14:21:29.20 Setting up PCR for ML -- writing /var/log/esp/ML20may2514h400ml.pcr @14:21:29.22 Building PCR Slug from ASV with MM2 mix, PP4 primer @14:21:29.23 Priming with carrier @14:21:29.24 ATV.dial ARV @14:21:29.82 ARV.dial :carrier @14:21:30.16 AS.seek 0.5ml @14:21:55.81 AFV.select :carrierSolution @14:21:55.92 delay 2 seconds @14:21:57.95 AS.reconfigure ASconfig @14:21:58.00 AS.seek 1ml @14:22:27.01 delay 1 second @14:22:28.03 AFV.select :holdingCoil @14:22:28.13 delay 1 second @14:22:29.16 ATV.dial :waste @14:22:30.73 AS.reconfigure ASnorm @14:22:30.78 AS.seek :empty @14:23:21.51 AFV.select :carrierSolution @14:23:21.62 delay 2 seconds @14:23:23.65 AS.reconfigure ASconfig @14:23:23.70 AS.seek 0.5ml @14:23:52.76 delay 1 second @14:23:53.78 AFV.select :holdingCoil @14:23:53.88 Building Slug @14:23:53.89 ATV.dial :ARV @14:23:55.45 delay 2 seconds @14:23:57.48 ARV.dial :air1 @14:23:57.87 delay 2 seconds @14:23:59.96 AS.reconfigure ASslow2 @14:24:00.02 AS.seek 0.506ml @14:24:06.26 delay 2 seconds @14:24:08.28 ARV.dial :MM2 @14:24:09.70 delay 2 seconds @14:24:11.73 Adding MM2 @14:24:11.80 AS.seek 0.524ml @14:24:30.03 delay 25 seconds @14:24:55.06 ARV.dial :PP4 @14:24:55.44 Adding PP4 @14:24:55.46 delay 2 seconds @14:24:57.54 AS.seek 0.53ml @14:25:04.58 delay 10 seconds @14:25:14.61 ARV.dial :air3 @14:25:15.00 adding ARV air @14:25:15.02 delay 2 seconds @14:25:17.11 AS.seek 0.536ml @14:25:23.56 delay 5 seconds @14:25:28.59 ARV.dial :carrier @14:25:29.81 delay 7 seconds @14:25:36.91 AS.seek 0.594ml @14:26:35.44 delay 5 seconds @14:26:40.47 ATV.dial :ASV @14:26:40.86 delay 2 seconds @14:26:42.96 AS.seek 0.6ml @14:26:49.31 delay 4 seconds @14:26:53.34 ATV.dial ARV @14:26:53.86 delay 2 seconds @14:26:55.89 ARV.dial :carrier @14:26:55.90 delay 2 seconds @14:26:58.00 AS.seek 0.65ml @14:27:48.61 delay 4 seconds @14:27:52.64 ATV.dial PCR @14:27:53.23 ASV.dial :waste1 @14:27:53.24 ARV.dial :air1 @14:27:53.64 Positioning Slug by 0.324ml @14:27:53.73 AS.seek 0.326ml @14:33:19.63 delay 5 seconds @14:33:24.66 ATV.dial ARV @14:33:25.41 ASV.dial :PCRlock @14:33:25.80 delay 5 seconds @14:33:30.83 Running ML Profile60C350 thermocycling @14:33:30.84 PCR.program Profile60C350 @14:33:30.96 Profile60C350 should complete in < 2:02:45 @14:33:30.97 PCR.run 2:38:26.25 @16:09:41.29 PCR.off @16:09:43.32 PCR for ML completed -- closed /var/log/esp/ML20may2514h400ml.pcr @16:09:43.39 Cleaning PCR @16:09:43.40 AFV.select :carrierSolution @16:09:43.51 delay 2 seconds @16:09:45.54 AS.reconfigure ASconfig @16:09:45.59 AS.seek 0.5ml @16:09:55.90 delay 1 second @16:09:56.92 AFV.select :holdingCoil @16:09:57.02 ASV.dial :waste1 @16:09:57.40 ATV.dial :air3 @16:09:58.64 AS.reconfigure ASslow1 @16:09:58.69 AS.seek 0.51ml @16:10:00.97 delay 2 seconds @16:10:02.99 ATV.dial :bleach @16:10:03.59 AS.seek 0.81ml @16:11:04.17 delay 3 seconds @16:11:07.20 ATV.dial :air3 @16:11:07.74 delay 3 seconds @16:11:10.83 AS.seek 0.835ml @16:11:16.13 delay 3 seconds @16:11:19.16 ATV.dial :bleach @16:11:19.77 AS.seek 0.82ml @16:11:23.04 delay 3 seconds @16:11:26.07 ARV.dial :waste @16:11:26.67 delay 3 seconds @16:11:29.70 ATV.dial :ARV @16:11:30.58 AS.reconfigure ASconfig @16:11:30.63 AS.seek 0.47ml @16:11:51.01 AFV.select :carrierSolution @16:11:51.12 delay 2 seconds @16:11:53.16 AS.seek 1ml @16:12:23.93 delay 1 second @16:12:24.95 AFV.select :holdingCoil @16:12:25.05 delay 1 second @16:12:26.08 AS.reconfigure ASfast @16:12:26.13 AS.seek 0ml @16:12:53.46 AFV.select :carrierSolution @16:12:53.57 delay 2 seconds @16:12:55.60 AS.reconfigure ASconfig @16:12:55.65 AS.seek 1ml @16:13:53.42 delay 1 second @16:13:54.44 AFV.select :holdingCoil @16:13:54.54 delay 1 second @16:13:55.57 AS.reconfigure ASfast @16:13:55.62 AS.seek 0ml @16:14:22.97 AFV.select :carrierSolution @16:14:23.08 delay 2 seconds @16:14:25.11 AS.reconfigure ASconfig @16:14:25.16 AS.seek 1ml @16:15:22.93 delay 1 second @16:15:23.95 AFV.select :holdingCoil @16:15:24.05 delay 1 second @16:15:25.08 AS.reconfigure ASfast @16:15:25.13 AS.seek 0ml @16:15:52.47 ARV.dial between carrier and air1 @16:15:52.97 ATV.dial :air3 @16:15:54.21 AS.reconfigure ASslow1 @16:15:54.26 AS.seek 0.01ml @16:15:56.59 delay 2 seconds @16:15:58.61 ATV.dial :bleach @16:15:59.22 AS.seek 0.81ml @16:18:40.36 delay 3 seconds @16:18:43.39 ATV.dial :air3 @16:18:43.92 delay 3 seconds @16:18:47.01 AS.seek 0.835ml @16:18:52.29 delay 3 seconds @16:18:55.32 ATV.dial :bleach @16:18:55.93 AS.seek 0.82ml @16:18:59.20 delay 3 seconds @16:19:02.23 ATV.dial PCR @16:19:02.62 AS.reconfigure ASconfig @16:19:02.67 AS.seek :empty @16:19:50.14 PCR.program :bleachProfile @16:19:50.20 PCR program should complete in < 2:45 @16:19:50.21 PCR.run 10:00 @16:23:04.15 PCR.off @16:23:04.17 AFV.select :carrierSolution @16:23:04.28 delay 2 seconds @16:23:06.32 AS.seek 1ml @16:24:04.13 delay 1 second @16:24:05.15 AFV.select :holdingCoil @16:24:05.25 delay 1 second @16:24:06.28 AS.reconfigure ASfast @16:24:07.09 AS.seek 0ml @16:24:34.44 delay 3 seconds @16:24:37.47 AFV.select :carrierSolution @16:24:37.57 delay 2 seconds @16:24:39.60 AS.reconfigure ASconfig @16:24:39.66 AS.seek 1ml @16:25:37.47 delay 1 second @16:25:38.49 AFV.select :holdingCoil @16:25:38.59 delay 1 second @16:25:39.62 AS.reconfigure ASfast @16:25:39.67 AS.seek 0ml @16:26:07.00 delay 3 seconds @16:26:10.03 AFV.select :carrierSolution @16:26:10.13 delay 2 seconds @16:26:12.17 AS.reconfigure ASconfig @16:26:12.22 AS.seek 1ml @16:27:10.02 delay 1 second @16:27:11.04 AFV.select :holdingCoil @16:27:11.14 delay 1 second @16:27:12.17 AS.reconfigure ASfast @16:27:12.22 AS.seek 0ml @16:27:39.57 delay 3 seconds @16:27:42.60 AFV.select :carrierSolution @16:27:42.70 delay 2 seconds @16:27:44.73 AS.reconfigure ASconfig @16:27:44.79 AS.seek 1ml @16:28:42.54 delay 1 second @16:28:43.56 AFV.select :holdingCoil @16:28:43.66 delay 1 second @16:28:44.69 AS.reconfigure ASfast @16:28:44.74 AS.seek 0ml @16:29:12.08 delay 3 seconds @16:29:15.11 AFV.select :carrierSolution @16:29:15.21 delay 2 seconds @16:29:17.24 AS.reconfigure ASconfig @16:29:17.29 AS.seek 1ml @16:30:15.09 delay 1 second @16:30:16.11 AFV.select :holdingCoil @16:30:16.21 delay 1 second @16:30:17.24 AS.reconfigure ASnorm @16:30:17.29 AS.seek 0ml @16:31:08.04 Setting up PCR for CG -- writing /var/log/esp/CG20may2516h400ml.pcr @16:31:08.06 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:31:08.07 Priming with carrier @16:31:08.08 ATV.dial ARV @16:31:08.66 ARV.dial :carrier @16:31:09.02 AS.seek 0.5ml @16:31:34.56 AFV.select :carrierSolution @16:31:34.67 delay 2 seconds @16:31:36.70 AS.reconfigure ASconfig @16:31:36.75 AS.seek 1ml @16:32:05.79 delay 1 second @16:32:06.81 AFV.select :holdingCoil @16:32:06.91 delay 1 second @16:32:07.94 ATV.dial :waste @16:32:09.52 AS.reconfigure ASnorm @16:32:09.57 AS.seek :empty @16:33:00.34 AFV.select :carrierSolution @16:33:00.45 delay 2 seconds @16:33:02.48 AS.reconfigure ASconfig @16:33:02.53 AS.seek 0.5ml @16:33:31.62 delay 1 second @16:33:32.64 AFV.select :holdingCoil @16:33:32.74 Building Slug @16:33:32.75 ATV.dial :ARV @16:33:34.31 delay 2 seconds @16:33:36.34 ARV.dial :air1 @16:33:36.74 delay 2 seconds @16:33:38.83 AS.reconfigure ASslow2 @16:33:38.88 AS.seek 0.506ml @16:33:45.29 delay 2 seconds @16:33:47.32 ARV.dial :MM1 @16:33:47.90 delay 2 seconds @16:33:49.93 Adding MM1 @16:33:50.00 AS.seek 0.524ml @16:34:08.31 delay 25 seconds @16:34:33.34 ARV.dial :PP2 @16:34:33.73 Adding PP2 @16:34:33.75 delay 2 seconds @16:34:35.83 AS.seek 0.53ml @16:34:42.07 delay 10 seconds @16:34:52.10 ARV.dial :air2 @16:34:52.48 adding ARV air @16:34:52.50 delay 2 seconds @16:34:54.58 AS.seek 0.536ml @16:35:00.81 delay 5 seconds @16:35:05.84 ARV.dial :carrier @16:35:07.06 delay 7 seconds @16:35:14.15 AS.seek 0.594ml @16:36:12.64 delay 5 seconds @16:36:17.67 ATV.dial :ASV @16:36:18.06 delay 2 seconds @16:36:20.15 AS.seek 0.6ml @16:36:26.45 delay 4 seconds @16:36:30.48 ATV.dial ARV @16:36:31.11 delay 2 seconds @16:36:33.14 ARV.dial :carrier @16:36:33.15 delay 2 seconds @16:36:35.24 AS.seek 0.65ml @16:37:26.09 delay 4 seconds @16:37:30.12 ATV.dial PCR @16:37:30.72 ASV.dial :waste1 @16:37:30.73 ARV.dial :air1 @16:37:31.13 Positioning Slug by 0.324ml @16:37:31.97 AS.seek 0.326ml @16:42:58.09 delay 5 seconds @16:43:03.12 ATV.dial ARV @16:43:03.88 ASV.dial :PCRlock @16:43:04.27 delay 5 seconds @16:43:09.30 Running CG Profile60C300 thermocycling @16:43:09.31 PCR.program Profile60C300 @16:43:09.41 Profile60C300 should complete in < 1:59:25 @16:43:09.43 PCR.run 2:34:16.25 @18:18:03.39 PCR.off @18:18:04.05 PCR for CG completed -- closed /var/log/esp/CG20may2516h400ml.pcr @18:18:04.12 Cleaning PCR @18:18:04.13 AFV.select :carrierSolution @18:18:04.24 delay 2 seconds @18:18:06.27 AS.reconfigure ASconfig @18:18:06.32 AS.seek 0.5ml @18:18:16.60 delay 1 second @18:18:17.62 AFV.select :holdingCoil @18:18:17.72 ASV.dial :waste1 @18:18:18.10 ATV.dial :air3 @18:18:19.34 AS.reconfigure ASslow1 @18:18:19.39 AS.seek 0.51ml @18:18:21.66 delay 2 seconds @18:18:23.68 ATV.dial :bleach @18:18:24.28 AS.seek 0.81ml @18:19:24.85 delay 3 seconds @18:19:27.88 ATV.dial :air3 @18:19:28.41 delay 3 seconds @18:19:31.50 AS.seek 0.835ml @18:19:36.80 delay 3 seconds @18:19:39.83 ATV.dial :bleach @18:19:40.44 AS.seek 0.82ml @18:19:43.76 delay 3 seconds @18:19:46.79 ARV.dial :waste @18:19:47.38 delay 3 seconds @18:19:50.41 ATV.dial :ARV @18:19:51.28 AS.reconfigure ASconfig @18:19:51.33 AS.seek 0.47ml @18:20:11.73 AFV.select :carrierSolution @18:20:11.85 delay 2 seconds @18:20:13.89 AS.seek 1ml @18:20:44.66 delay 1 second @18:20:45.68 AFV.select :holdingCoil @18:20:45.78 delay 1 second @18:20:46.81 AS.reconfigure ASfast @18:20:46.86 AS.seek 0ml @18:21:14.20 AFV.select :carrierSolution @18:21:14.31 delay 2 seconds @18:21:16.34 AS.reconfigure ASconfig @18:21:16.39 AS.seek 1ml @18:22:14.17 delay 1 second @18:22:15.19 AFV.select :holdingCoil @18:22:16.06 delay 1 second @18:22:17.09 AS.reconfigure ASfast @18:22:17.14 AS.seek 0ml @18:22:44.48 AFV.select :carrierSolution @18:22:44.59 delay 2 seconds @18:22:46.62 AS.reconfigure ASconfig @18:22:46.67 AS.seek 1ml @18:23:44.45 delay 1 second @18:23:45.47 AFV.select :holdingCoil @18:23:45.57 delay 1 second @18:23:46.60 AS.reconfigure ASfast @18:23:46.65 AS.seek 0ml @18:24:13.98 ARV.dial between carrier and air1 @18:24:14.48 ATV.dial :air3 @18:24:15.72 AS.reconfigure ASslow1 @18:24:15.77 AS.seek 0.01ml @18:24:18.06 delay 2 seconds @18:24:20.08 ATV.dial :bleach @18:24:20.69 AS.seek 0.81ml @18:27:01.82 delay 3 seconds @18:27:04.85 ATV.dial :air3 @18:27:05.39 delay 3 seconds @18:27:08.48 AS.seek 0.835ml @18:27:13.79 delay 3 seconds @18:27:16.82 ATV.dial :bleach @18:27:17.43 AS.seek 0.82ml @18:27:20.75 delay 3 seconds @18:27:23.78 ATV.dial PCR @18:27:24.17 AS.reconfigure ASconfig @18:27:24.22 AS.seek :empty @18:28:11.67 PCR.program :bleachProfile @18:28:11.73 PCR program should complete in < 2:45 @18:28:11.74 PCR.run 10:00 @18:31:25.68 PCR.off @18:31:25.71 AFV.select :carrierSolution @18:31:25.82 delay 2 seconds @18:31:27.86 AS.seek 1ml @18:32:25.66 delay 1 second @18:32:26.68 AFV.select :holdingCoil @18:32:26.78 delay 1 second @18:32:27.81 AS.reconfigure ASfast @18:32:27.86 AS.seek 0ml @18:32:55.22 delay 3 seconds @18:32:58.25 AFV.select :carrierSolution @18:32:58.35 delay 2 seconds @18:33:00.38 AS.reconfigure ASconfig @18:33:00.47 AS.seek 1ml @18:33:58.25 delay 1 second @18:33:59.27 AFV.select :holdingCoil @18:33:59.37 delay 1 second @18:34:00.40 AS.reconfigure ASfast @18:34:00.45 AS.seek 0ml @18:34:27.80 delay 3 seconds @18:34:30.83 AFV.select :carrierSolution @18:34:30.93 delay 2 seconds @18:34:32.96 AS.reconfigure ASconfig @18:34:33.01 AS.seek 1ml @18:35:30.85 delay 1 second @18:35:31.87 AFV.select :holdingCoil @18:35:31.97 delay 1 second @18:35:33.00 AS.reconfigure ASfast @18:35:33.05 AS.seek 0ml @18:36:00.41 delay 3 seconds @18:36:03.44 AFV.select :carrierSolution @18:36:03.54 delay 2 seconds @18:36:05.57 AS.reconfigure ASconfig @18:36:05.62 AS.seek 1ml @18:37:03.39 delay 1 second @18:37:04.41 AFV.select :holdingCoil @18:37:04.51 delay 1 second @18:37:05.54 AS.reconfigure ASfast @18:37:05.59 AS.seek 0ml @18:37:32.94 delay 3 seconds @18:37:35.97 AFV.select :carrierSolution @18:37:36.07 delay 2 seconds @18:37:38.10 AS.reconfigure ASconfig @18:37:38.16 AS.seek 1ml @18:38:35.99 delay 1 second @18:38:37.01 AFV.select :holdingCoil @18:38:37.11 delay 1 second @18:38:38.14 AS.reconfigure ASnorm @18:38:38.19 AS.seek 0ml @18:39:28.93 Setting up PCR for NM -- writing /var/log/esp/NM20may2518h400ml.pcr @18:39:28.95 Building PCR Slug from ASV with MM1 mix, PP1 primer @18:39:28.96 Priming with carrier @18:39:28.97 ATV.dial ARV @18:39:29.66 ARV.dial :carrier @18:39:30.01 AS.seek 0.5ml @18:39:55.60 AFV.select :carrierSolution @18:39:55.71 delay 2 seconds @18:39:57.74 AS.reconfigure ASconfig @18:39:57.81 AS.seek 1ml @18:40:26.85 delay 1 second @18:40:27.87 AFV.select :holdingCoil @18:40:27.97 delay 1 second @18:40:29.00 ATV.dial :waste @18:40:31.31 AS.reconfigure ASnorm @18:40:31.39 AS.seek :empty @18:41:22.13 AFV.select :carrierSolution @18:41:22.24 delay 2 seconds @18:41:24.27 AS.reconfigure ASconfig @18:41:24.32 AS.seek 0.5ml @18:41:53.43 delay 1 second @18:41:54.45 AFV.select :holdingCoil @18:41:54.55 Building Slug @18:41:54.56 ATV.dial :ARV @18:41:56.12 delay 2 seconds @18:41:58.15 ARV.dial :air1 @18:41:58.55 delay 2 seconds @18:42:00.64 AS.reconfigure ASslow2 @18:42:00.70 AS.seek 0.506ml @18:42:06.96 delay 2 seconds @18:42:08.99 ARV.dial :MM1 @18:42:09.57 delay 2 seconds @18:42:11.60 Adding MM1 @18:42:11.67 AS.seek 0.524ml @18:42:30.13 delay 25 seconds @18:42:55.16 ARV.dial :PP1 @18:42:55.54 Adding PP1 @18:42:55.56 delay 2 seconds @18:42:57.65 AS.seek 0.53ml @18:43:04.09 delay 10 seconds @18:43:14.12 ARV.dial :air1 @18:43:14.51 adding ARV air @18:43:14.53 delay 2 seconds @18:43:16.61 AS.seek 0.536ml @18:43:22.87 delay 5 seconds @18:43:27.90 ARV.dial :carrier @18:43:28.29 delay 7 seconds @18:43:35.38 AS.seek 0.594ml @18:44:33.93 delay 5 seconds @18:44:38.96 ATV.dial :ASV @18:44:39.35 delay 2 seconds @18:44:41.44 AS.seek 0.6ml @18:44:47.77 delay 4 seconds @18:44:51.80 ATV.dial ARV @18:44:52.34 delay 2 seconds @18:44:54.37 ARV.dial :carrier @18:44:54.38 delay 2 seconds @18:44:56.47 AS.seek 0.65ml @18:45:46.90 delay 4 seconds @18:45:50.93 ATV.dial PCR @18:45:51.51 ASV.dial :waste1 @18:45:51.52 ARV.dial :air1 @18:45:51.93 Positioning Slug by 0.324ml @18:45:52.02 AS.seek 0.326ml @18:51:18.09 delay 5 seconds @18:51:23.12 ATV.dial ARV @18:51:23.87 ASV.dial :PCRlock @18:51:24.25 delay 5 seconds @18:51:29.28 Running NM Profile60C300 thermocycling @18:51:29.29 PCR.program Profile60C300 @18:51:29.40 Profile60C300 should complete in < 1:59:25 @18:51:29.41 PCR.run 2:34:16.25 @20:26:32.40 PCR.off @20:26:33.08 PCR for NM completed -- closed /var/log/esp/NM20may2518h400ml.pcr @20:26:33.15 Cleaning PCR @20:26:33.16 AFV.select :carrierSolution @20:26:33.27 delay 2 seconds @20:26:35.30 AS.reconfigure ASconfig @20:26:35.35 AS.seek 0.5ml @20:26:45.65 delay 1 second @20:26:46.67 AFV.select :holdingCoil @20:26:46.77 ASV.dial :waste1 @20:26:47.16 ATV.dial :air3 @20:26:48.40 AS.reconfigure ASslow1 @20:26:48.45 AS.seek 0.51ml @20:26:50.73 delay 2 seconds @20:26:52.75 ATV.dial :bleach @20:26:53.36 AS.seek 0.81ml @20:27:53.97 delay 3 seconds @20:27:57.00 ATV.dial :air3 @20:27:57.54 delay 3 seconds @20:28:00.63 AS.seek 0.835ml @20:28:05.91 delay 3 seconds @20:28:08.94 ATV.dial :bleach @20:28:09.54 AS.seek 0.82ml @20:28:12.86 delay 3 seconds @20:28:15.89 ARV.dial :waste @20:28:16.49 delay 3 seconds @20:28:19.52 ATV.dial :ARV @20:28:20.39 AS.reconfigure ASconfig @20:28:20.44 AS.seek 0.47ml @20:28:40.85 AFV.select :carrierSolution @20:28:40.96 delay 2 seconds @20:28:43.00 AS.seek 1ml @20:29:13.77 delay 1 second @20:29:14.79 AFV.select :holdingCoil @20:29:14.89 delay 1 second @20:29:15.92 AS.reconfigure ASfast @20:29:15.97 AS.seek 0ml @20:29:43.30 AFV.select :carrierSolution @20:29:43.41 delay 2 seconds @20:29:45.44 AS.reconfigure ASconfig @20:29:45.49 AS.seek 1ml @20:30:43.34 delay 1 second @20:30:44.36 AFV.select :holdingCoil @20:30:44.46 delay 1 second @20:30:45.49 AS.reconfigure ASfast @20:30:45.54 AS.seek 0ml @20:31:12.88 AFV.select :carrierSolution @20:31:12.99 delay 2 seconds @20:31:15.02 AS.reconfigure ASconfig @20:31:15.07 AS.seek 1ml @20:32:12.84 delay 1 second @20:32:13.86 AFV.select :holdingCoil @20:32:13.96 delay 1 second @20:32:14.99 AS.reconfigure ASfast @20:32:15.04 AS.seek 0ml @20:32:42.38 ARV.dial between carrier and air1 @20:32:42.87 ATV.dial :air3 @20:32:44.11 AS.reconfigure ASslow1 @20:32:44.16 AS.seek 0.01ml @20:32:46.49 delay 2 seconds @20:32:48.51 ATV.dial :bleach @20:32:49.11 AS.seek 0.81ml @20:35:30.23 delay 3 seconds @20:35:33.26 ATV.dial :air3 @20:35:33.79 delay 3 seconds @20:35:36.88 AS.seek 0.835ml @20:35:42.18 delay 3 seconds @20:35:45.21 ATV.dial :bleach @20:35:45.82 AS.seek 0.82ml @20:35:49.14 delay 3 seconds @20:35:52.17 ATV.dial PCR @20:35:52.57 AS.reconfigure ASconfig @20:35:52.63 AS.seek :empty @20:36:40.09 PCR.program :bleachProfile @20:36:40.14 PCR program should complete in < 2:45 @20:36:40.16 PCR.run 10:00 @20:39:54.84 PCR.off @20:39:54.86 AFV.select :carrierSolution @20:39:54.97 delay 2 seconds @20:39:57.01 AS.seek 1ml @20:40:54.83 delay 1 second @20:40:55.85 AFV.select :holdingCoil @20:40:55.95 delay 1 second @20:40:56.98 AS.reconfigure ASfast @20:40:57.03 AS.seek 0ml @20:41:24.37 delay 3 seconds @20:41:27.40 AFV.select :carrierSolution @20:41:27.50 delay 2 seconds @20:41:29.53 AS.reconfigure ASconfig @20:41:29.58 AS.seek 1ml @20:42:27.33 delay 1 second @20:42:28.35 AFV.select :holdingCoil @20:42:28.45 delay 1 second @20:42:29.48 AS.reconfigure ASfast @20:42:29.53 AS.seek 0ml @20:42:56.88 delay 3 seconds @20:42:59.91 AFV.select :carrierSolution @20:43:00.01 delay 2 seconds @20:43:02.04 AS.reconfigure ASconfig @20:43:02.10 AS.seek 1ml @20:43:59.84 delay 1 second @20:44:00.86 AFV.select :holdingCoil @20:44:00.96 delay 1 second @20:44:01.99 AS.reconfigure ASfast @20:44:02.04 AS.seek 0ml @20:44:29.36 delay 3 seconds @20:44:32.39 AFV.select :carrierSolution @20:44:32.50 delay 2 seconds @20:44:34.53 AS.reconfigure ASconfig @20:44:34.58 AS.seek 1ml @20:45:32.38 delay 1 second @20:45:33.40 AFV.select :holdingCoil @20:45:33.50 delay 1 second @20:45:34.53 AS.reconfigure ASfast @20:45:34.58 AS.seek 0ml @20:46:01.94 delay 3 seconds @20:46:04.97 AFV.select :carrierSolution @20:46:05.07 delay 2 seconds @20:46:07.10 AS.reconfigure ASconfig @20:46:07.15 AS.seek 1ml @20:47:04.93 delay 1 second @20:47:05.95 AFV.select :holdingCoil @20:47:06.05 delay 1 second @20:47:07.08 AS.reconfigure ASnorm @20:47:07.13 AS.seek 0ml @20:47:57.84 Setting up PCR for IPc -- writing /var/log/esp/IPc20may2520h400ml.pcr @20:47:57.86 Building PCR Slug from ASV with MM3 mix, PP6 primer @20:47:57.87 Priming with carrier @20:47:57.88 ATV.dial ARV @20:47:58.47 ARV.dial :carrier @20:47:58.82 AS.seek 0.5ml @20:48:24.44 AFV.select :carrierSolution @20:48:24.55 delay 2 seconds @20:48:26.58 AS.reconfigure ASconfig @20:48:26.63 AS.seek 1ml @20:48:55.65 delay 1 second @20:48:56.67 AFV.select :holdingCoil @20:48:56.77 delay 1 second @20:48:57.80 ATV.dial :waste @20:48:59.36 AS.reconfigure ASnorm @20:48:59.42 AS.seek :empty @20:49:50.18 AFV.select :carrierSolution @20:49:50.29 delay 2 seconds @20:49:52.32 AS.reconfigure ASconfig @20:49:52.37 AS.seek 0.5ml @20:50:21.46 delay 1 second @20:50:22.48 AFV.select :holdingCoil @20:50:22.58 Building Slug @20:50:22.59 ATV.dial :ARV @20:50:24.15 delay 2 seconds @20:50:26.18 ARV.dial :air1 @20:50:26.58 delay 2 seconds @20:50:28.67 AS.reconfigure ASslow2 @20:50:28.72 AS.seek 0.506ml @20:50:35.10 delay 2 seconds @20:50:37.12 ARV.dial :MM3 @20:50:38.13 delay 2 seconds @20:50:40.16 Adding MM3 @20:50:40.24 AS.seek 0.524ml @20:50:58.71 delay 25 seconds @20:51:23.74 ARV.dial :PP6 @20:51:24.12 Adding PP6 @20:51:24.14 delay 2 seconds @20:51:26.22 AS.seek 0.53ml @20:51:32.40 delay 10 seconds @20:51:42.43 ARV.dial :air1 @20:51:43.24 adding ARV air @20:51:43.25 delay 2 seconds @20:51:45.34 AS.seek 0.536ml @20:51:51.57 delay 5 seconds @20:51:56.60 ARV.dial :carrier @20:51:57.01 delay 7 seconds @20:52:04.10 AS.seek 0.594ml @20:53:02.65 delay 5 seconds @20:53:07.68 ATV.dial :ASV @20:53:08.07 delay 2 seconds @20:53:10.16 AS.seek 0.6ml @20:53:16.63 delay 4 seconds @20:53:20.66 ATV.dial ARV @20:53:21.94 delay 2 seconds @20:53:23.97 ARV.dial :carrier @20:53:23.98 delay 2 seconds @20:53:26.07 AS.seek 0.65ml @20:54:16.74 delay 4 seconds @20:54:20.77 ATV.dial PCR @20:54:21.37 ASV.dial :waste1 @20:54:21.38 ARV.dial :air1 @20:54:21.78 Positioning Slug by 0.324ml @20:54:21.87 AS.seek 0.326ml @20:59:47.97 delay 5 seconds @20:59:53.00 ATV.dial ARV @20:59:53.75 ASV.dial :PCRlock @20:59:54.14 delay 5 seconds @20:59:59.17 Running IPc ProfileIPc thermocycling @20:59:59.18 PCR.program ProfileIPc @20:59:59.29 ProfileIPc should complete in < 1:36:15 @20:59:59.30 PCR.run 2:05:18.75 @22:18:01.71 PCR.off @22:18:02.27 PCR for IPc completed -- closed /var/log/esp/IPc20may2520h400ml.pcr @22:18:02.34 Cleaning PCR @22:18:02.35 AFV.select :carrierSolution @22:18:02.46 delay 2 seconds @22:18:04.49 AS.reconfigure ASconfig @22:18:04.54 AS.seek 0.5ml @22:18:14.83 delay 1 second @22:18:15.85 AFV.select :holdingCoil @22:18:15.95 ASV.dial :waste1 @22:18:16.33 ATV.dial :air3 @22:18:17.56 AS.reconfigure ASslow1 @22:18:18.39 AS.seek 0.51ml @22:18:20.66 delay 2 seconds @22:18:22.68 ATV.dial :bleach @22:18:23.31 AS.seek 0.81ml @22:19:23.94 delay 3 seconds @22:19:26.97 ATV.dial :air3 @22:19:27.51 delay 3 seconds @22:19:30.60 AS.seek 0.835ml @22:19:35.89 delay 3 seconds @22:19:38.92 ATV.dial :bleach @22:19:39.53 AS.seek 0.82ml @22:19:42.83 delay 3 seconds @22:19:45.86 ARV.dial :waste @22:19:46.44 delay 3 seconds @22:19:49.47 ATV.dial :ARV @22:19:50.34 AS.reconfigure ASconfig @22:19:50.39 AS.seek 0.47ml @22:20:10.82 AFV.select :carrierSolution @22:20:10.93 delay 2 seconds @22:20:12.97 AS.seek 1ml @22:20:43.76 delay 1 second @22:20:44.78 AFV.select :holdingCoil @22:20:44.88 delay 1 second @22:20:45.91 AS.reconfigure ASfast @22:20:45.96 AS.seek 0ml @22:21:13.30 AFV.select :carrierSolution @22:21:13.41 delay 2 seconds @22:21:15.44 AS.reconfigure ASconfig @22:21:15.49 AS.seek 1ml @22:22:13.27 delay 1 second @22:22:14.29 AFV.select :holdingCoil @22:22:14.39 delay 1 second @22:22:15.42 AS.reconfigure ASfast @22:22:15.47 AS.seek 0ml @22:22:42.81 AFV.select :carrierSolution @22:22:42.92 delay 2 seconds @22:22:44.95 AS.reconfigure ASconfig @22:22:45.00 AS.seek 1ml @22:23:42.78 delay 1 second @22:23:43.80 AFV.select :holdingCoil @22:23:43.90 delay 1 second @22:23:44.93 AS.reconfigure ASfast @22:23:44.98 AS.seek 0ml @22:24:12.32 ARV.dial between carrier and air1 @22:24:12.79 ATV.dial :air3 @22:24:14.03 AS.reconfigure ASslow1 @22:24:14.08 AS.seek 0.01ml @22:24:16.40 delay 2 seconds @22:24:18.42 ATV.dial :bleach @22:24:19.03 AS.seek 0.81ml @22:27:00.17 delay 3 seconds @22:27:03.20 ATV.dial :air3 @22:27:03.73 delay 3 seconds @22:27:06.82 AS.seek 0.835ml @22:27:12.13 delay 3 seconds @22:27:15.16 ATV.dial :bleach @22:27:15.77 AS.seek 0.82ml @22:27:19.03 delay 3 seconds @22:27:22.06 ATV.dial PCR @22:27:22.45 AS.reconfigure ASconfig @22:27:22.51 AS.seek :empty @22:28:09.93 PCR.program :bleachProfile @22:28:09.98 PCR program should complete in < 2:45 @22:28:09.99 PCR.run 10:00 @22:31:23.93 PCR.off @22:31:23.95 AFV.select :carrierSolution @22:31:24.06 delay 2 seconds @22:31:26.10 AS.seek 1ml @22:32:23.89 delay 1 second @22:32:24.91 AFV.select :holdingCoil @22:32:25.01 delay 1 second @22:32:26.04 AS.reconfigure ASfast @22:32:26.09 AS.seek 0ml @22:32:53.44 delay 3 seconds @22:32:56.47 AFV.select :carrierSolution @22:32:56.57 delay 2 seconds @22:32:58.60 AS.reconfigure ASconfig @22:32:58.66 AS.seek 1ml @22:33:56.44 delay 1 second @22:33:57.46 AFV.select :holdingCoil @22:33:57.56 delay 1 second @22:33:58.59 AS.reconfigure ASfast @22:33:58.64 AS.seek 0ml @22:34:25.99 delay 3 seconds @22:34:29.02 AFV.select :carrierSolution @22:34:29.12 delay 2 seconds @22:34:31.15 AS.reconfigure ASconfig @22:34:31.21 AS.seek 1ml @22:35:29.03 delay 1 second @22:35:30.05 AFV.select :holdingCoil @22:35:30.15 delay 1 second @22:35:31.89 AS.reconfigure ASfast @22:35:31.98 AS.seek 0ml @22:35:59.32 delay 3 seconds @22:36:02.35 AFV.select :carrierSolution @22:36:02.46 delay 2 seconds @22:36:04.49 AS.reconfigure ASconfig @22:36:04.54 AS.seek 1ml @22:37:02.30 delay 1 second @22:37:03.32 AFV.select :holdingCoil @22:37:03.42 delay 1 second @22:37:04.45 AS.reconfigure ASfast @22:37:04.50 AS.seek 0ml @22:37:31.86 delay 3 seconds @22:37:34.89 AFV.select :carrierSolution @22:37:34.99 delay 2 seconds @22:37:37.02 AS.reconfigure ASconfig @22:37:37.08 AS.seek 1ml @22:38:34.88 delay 1 second @22:38:35.90 AFV.select :holdingCoil @22:38:36.00 delay 1 second @22:38:37.03 AS.reconfigure ASnorm @22:38:37.08 AS.seek 0ml @22:39:27.82 Setting up PCR for IPc2 -- writing /var/log/esp/IPc220may2522h400ml.pcr @22:39:27.84 Building PCR Slug from ASV with MM2 mix, PP3 primer @22:39:27.85 Priming with carrier @22:39:27.86 ATV.dial ARV @22:39:28.57 ARV.dial :carrier @22:39:28.92 AS.seek 0.5ml @22:39:54.46 AFV.select :carrierSolution @22:39:54.57 delay 2 seconds @22:39:56.61 AS.reconfigure ASconfig @22:39:56.65 AS.seek 1ml @22:40:25.71 delay 1 second @22:40:26.73 AFV.select :holdingCoil @22:40:26.83 delay 1 second @22:40:27.86 ATV.dial :waste @22:40:29.44 AS.reconfigure ASnorm @22:40:29.49 AS.seek :empty @22:41:20.24 AFV.select :carrierSolution @22:41:20.35 delay 2 seconds @22:41:22.38 AS.reconfigure ASconfig @22:41:22.43 AS.seek 0.5ml @22:41:51.51 delay 1 second @22:41:52.53 AFV.select :holdingCoil @22:41:52.63 Building Slug @22:41:52.64 ATV.dial :ARV @22:41:54.30 delay 2 seconds @22:41:56.33 ARV.dial :air1 @22:41:56.72 delay 2 seconds @22:41:58.81 AS.reconfigure ASslow2 @22:41:58.86 AS.seek 0.506ml @22:42:05.24 delay 2 seconds @22:42:07.27 ARV.dial :MM2 @22:42:08.69 delay 2 seconds @22:42:10.72 Adding MM2 @22:42:10.79 AS.seek 0.524ml @22:42:29.21 delay 25 seconds @22:42:54.24 ARV.dial :PP3 @22:42:54.62 Adding PP3 @22:42:54.63 delay 2 seconds @22:42:56.72 AS.seek 0.53ml @22:43:03.01 delay 10 seconds @22:43:13.04 ARV.dial :air2 @22:43:13.43 adding ARV air @22:43:13.45 delay 2 seconds @22:43:15.53 AS.seek 0.536ml @22:43:21.96 delay 5 seconds @22:43:26.99 ARV.dial :carrier @22:43:28.23 delay 7 seconds @22:43:35.32 AS.seek 0.594ml @22:44:34.04 delay 5 seconds @22:44:39.07 ATV.dial :ASV @22:44:39.46 delay 2 seconds @22:44:41.55 AS.seek 0.6ml @22:44:47.95 delay 4 seconds @22:44:51.98 ATV.dial ARV @22:44:52.52 delay 2 seconds @22:44:54.55 ARV.dial :carrier @22:44:54.56 delay 2 seconds @22:44:56.65 AS.seek 0.65ml @22:45:47.07 delay 4 seconds @22:45:51.10 ATV.dial PCR @22:45:51.68 ASV.dial :waste1 @22:45:51.69 ARV.dial :air1 @22:45:52.19 Positioning Slug by 0.324ml @22:45:52.28 AS.seek 0.326ml @22:51:18.37 delay 5 seconds @22:51:23.41 ATV.dial ARV @22:51:24.15 ASV.dial :PCRlock @22:51:24.53 delay 5 seconds @22:51:29.56 Running IPc2 ProfileIPc thermocycling @22:51:29.57 PCR.program ProfileIPc @22:51:29.68 ProfileIPc should complete in < 1:36:15 @22:51:29.69 PCR.run 2:05:18.75 @00:09:29.91CEST26-May-20 PCR.off @00:09:30.46 PCR for IPc2 completed -- closed /var/log/esp/IPc220may2522h400ml.pcr @00:09:30.48 Full MFB Decontamination @00:09:30.49 Decon extraction column and decon AIV @00:09:30.51 SPE.hold 2:46:40,55C @00:09:30.56 ASV.dial :SPEdelivery @00:09:31.21 delay 20 seconds @00:09:51.24 AFV.select :carrierSolution @00:09:51.34 delay 2 seconds @00:09:53.37 AS.reconfigure ASconfig @00:09:53.42 AS.seek 0.5ml @00:10:03.72 delay 1 second @00:10:04.74 AFV.select :holdingCoil @00:10:04.84 ATV.dial :air1 @00:10:06.75 AS.reconfigure ASslow1 @00:10:06.80 AS.seek 0.52ml @00:10:11.08 ATV.dial :ASV @00:10:12.85 ASV.dial :SPErecovery @00:10:13.55 AS.seek 0.62ml @00:10:33.94 ATV.dial :waste @00:10:35.91 AS.reconfigure ASnorm @00:10:35.96 AS.seek :empty @00:11:07.60 AFV.select :holdingCoil @00:11:07.61 ATV.dial :waste @00:11:07.63 AS.reconfigure ASconfig @00:11:07.68 AS.seek 0ml @00:11:07.98 AFV.select :carrierSolution @00:11:08.10 delay 2 seconds @00:11:10.14 AS.seek 0.5ml @00:11:39.16 delay 1 second @00:11:40.18 AFV.select :holdingCoil @00:11:40.28 ATV.dial :air3 @00:11:41.01 AS.reconfigure ASslow1 @00:11:41.06 AS.seek 0.52ml @00:11:45.37 ATV.dial :air3 @00:11:46.23 AS.seek 0.53ml @00:11:48.46 delay 2 seconds @00:11:50.48 ATV.dial :bleach @00:11:50.97 AS.seek 0.71ml @00:12:27.38 delay 3 seconds @00:12:30.41 ATV.dial :air3 @00:12:30.99 delay 3 seconds @00:12:34.08 AS.seek 0.735ml @00:12:39.39 delay 3 seconds @00:12:42.42 ATV.dial :bleach @00:12:43.08 AS.seek 0.72ml @00:12:46.34 delay 3 seconds @00:12:49.37 ATV.dial ASV @00:12:50.46 ASV.dial :SPErecovery @00:12:50.55 AS.seek 0.365ml @00:14:02.23 ATV.dial :waste @00:14:03.96 AS.reconfigure ASnorm @00:14:04.01 AS.seek :empty @00:14:22.81 ATV.dial :air3 @00:14:23.52 AS.reconfigure ASslow1 @00:14:23.57 AS.seek 0.009ml @00:14:25.93 delay 2 seconds @00:14:27.95 ATV.dial :bleach @00:14:28.42 AS.seek 0.579ml @00:16:23.29 delay 3 seconds @00:16:26.32 ATV.dial :air3 @00:16:26.87 delay 3 seconds @00:16:29.96 AS.seek 0.604ml @00:16:35.32 delay 3 seconds @00:16:38.34 ATV.dial :bleach @00:16:38.94 AS.seek 0.589ml @00:16:42.20 delay 3 seconds @00:16:45.23 ATV.dial :ASV @00:16:46.26 ASV.dial :wasteR @00:16:46.66 AS.seek 0ml @00:18:45.42 delay 1:00 @00:19:45.45 ATV.dial ASV @00:19:45.46 AFV.select :carrierSolution @00:19:45.56 delay 2 seconds @00:19:47.59 AS.reconfigure ASconfig @00:19:47.64 AS.seek 1ml @00:20:45.40 delay 1 second @00:20:46.42 AFV.select :holdingCoil @00:20:46.52 delay 1 second @00:20:47.55 ASV.dial :wasteR @00:20:47.57 AS.reconfigure ASslow1 @00:20:47.61 AS.seek 0ml @00:24:09.00 delay 10 seconds @00:24:19.03 ATV.dial :air3 @00:24:20.49 AS.seek 0.01ml @00:24:22.77 delay 2 seconds @00:24:24.79 ATV.dial :bleach @00:24:25.41 AS.seek 0.58ml @00:26:20.31 delay 3 seconds @00:26:23.34 ATV.dial :air3 @00:26:23.88 delay 3 seconds @00:26:26.97 AS.seek 0.605ml @00:26:32.31 delay 3 seconds @00:26:35.34 ATV.dial :bleach @00:26:35.95 AS.seek 0.59ml @00:26:39.24 delay 3 seconds @00:26:42.27 ATV.dial ASV @00:26:43.30 ASV.dial :SPEdelivery @00:26:43.68 AS.seek 0ml @00:28:42.61 delay 1:00 @00:29:42.64 AFV.select :carrierSolution @00:29:42.74 delay 2 seconds @00:29:44.77 AS.reconfigure ASconfig @00:29:44.82 AS.seek 1ml @00:30:42.58 delay 1 second @00:30:43.60 AFV.select :holdingCoil @00:30:43.70 delay 1 second @00:30:44.73 ATV.dial ASV @00:30:44.74 ASV.dial :SPEdelivery @00:30:44.76 AS.reconfigure ASslow1 @00:30:44.80 AS.seek 0ml @00:34:06.21 delay 10 seconds @00:34:16.24 ATV.dial :air2 @00:34:17.05 AS.reconfigure ASslow0 @00:34:17.09 AS.seek 0.8ml @00:35:37.82 delay 3 seconds @00:35:40.85 ATV.dial :ASV @00:35:41.80 ASV.dial :SPEdelivery @00:35:41.82 AS.reconfigure ASslow1 @00:35:41.87 AS.seek 0.03ml @00:38:16.99 delay 20 seconds @00:38:37.02 ATV.dial :waste,via: :air3 @00:38:39.45 AS.reconfigure ASconfig @00:38:39.50 AS.seek :empty @00:38:41.56 SPE.coast @00:38:41.66 Removing Bleach from Mixing Coil @00:38:41.67 AFV.select :carrierSolution @00:38:41.77 delay 2 seconds @00:38:43.81 AS.seek 0.5ml @00:39:12.90 delay 1 second @00:39:13.92 AFV.select :holdingCoil @00:39:14.02 ATV.dial :ASV @00:39:15.63 ASV.dial :SPErecovery @00:39:16.27 AS.reconfigure ASslow1 @00:39:16.32 AS.seek 0.73ml @00:40:02.85 ASV.dial :wasteD @00:40:03.43 AS.reconfigure ASnorm @00:40:03.48 AS.seek 0ml @00:40:40.59 AFV.select :carrierSolution @00:40:40.70 delay 2 seconds @00:40:42.73 AS.reconfigure ASconfig @00:40:42.78 AS.seek 0.5ml @00:41:11.84 delay 1 second @00:41:12.86 AFV.select :holdingCoil @00:41:12.96 ATV.dial ASV @00:41:12.97 ASV.dial :SPErecovery @00:41:13.61 AS.reconfigure ASslow0 @00:41:13.66 AS.seek 0.3ml @00:41:34.14 ATV.dial :air2 @00:41:35.16 AS.reconfigure ASslow1 @00:41:35.21 AS.seek 0.32ml @00:41:39.59 ATV.dial ASV @00:41:40.60 AS.reconfigure ASslow0 @00:41:40.65 AS.seek 0.57ml @00:42:06.18 ASV.dial :wasteD @00:42:06.76 AS.reconfigure ASnorm @00:42:06.80 AS.seek 0ml @00:42:35.89 AFV.select :carrierSolution @00:42:36.00 delay 2 seconds @00:42:38.03 AS.reconfigure ASconfig @00:42:38.08 AS.seek 0.5ml @00:43:07.15 delay 1 second @00:43:08.17 AFV.select :holdingCoil @00:43:08.27 ATV.dial ASV @00:43:08.28 ASV.dial :SPErecovery @00:43:08.93 AS.reconfigure ASslow0 @00:43:08.98 AS.seek 0.3ml @00:43:29.43 ATV.dial :air2 @00:43:30.44 AS.reconfigure ASslow1 @00:43:30.49 AS.seek 0.32ml @00:43:34.82 ATV.dial ASV @00:43:35.83 AS.reconfigure ASslow0 @00:43:35.88 AS.seek 0.57ml @00:44:01.34 ASV.dial :wasteD @00:44:01.92 AS.reconfigure ASnorm @00:44:01.97 AS.seek 0ml @00:44:31.06 ATV.dial :air2 @00:44:32.01 AS.reconfigure ASslow0 @00:44:32.06 AS.seek 0.3ml @00:45:02.57 ATV.dial :ASV @00:45:03.51 ASV.dial :wasteD @00:45:03.53 AS.reconfigure ASconfig @00:45:03.58 AS.seek 0.05ml @00:45:18.28 ATV.dial :waste @00:45:20.09 AS.seek 0ml @00:45:23.26 ASV.dial :SPEdelivery @00:45:24.25 deconLysateINT @00:45:24.26 ATV.dial :air3 @00:45:24.94 AS.reconfigure ASslow1 @00:45:24.99 AS.seek 0.01ml @00:45:27.39 delay 2 seconds @00:45:29.41 ATV.dial :bleach @00:45:29.86 AS.seek 0.96ml @00:48:41.19 delay 3 seconds @00:48:44.22 ATV.dial :air3 @00:48:44.76 delay 3 seconds @00:48:47.85 AS.seek 0.985ml @00:48:53.13 delay 3 seconds @00:48:56.16 ATV.dial :bleach @00:48:56.77 AS.seek 0.97ml @00:49:00.05 delay 3 seconds @00:49:03.08 ATV.dial AIV @00:49:04.30 AIV.dial :coilToMFB @00:49:04.32 AWV.select :waste @00:49:04.33 AFV.select :carrierSolution @00:49:04.44 delay 2 seconds @00:49:07.20 AS.reconfigure ASconfig @00:49:07.28 AS.seek 1ml @00:49:09.31 delay 1 second @00:49:10.33 AFV.select :holdingCoil @00:49:10.43 delay 1 second @00:49:11.46 AS.reconfigure ASslow0 @00:49:11.51 AS.seek :empty @00:50:52.48 AFV.select :carrierSolution @00:50:52.59 delay 2 seconds @00:50:54.62 AS.reconfigure ASconfig @00:50:54.67 AS.seek 1ml @00:51:52.49 delay 1 second @00:51:53.51 AFV.select :holdingCoil @00:51:53.61 delay 1 second @00:51:54.64 AS.reconfigure ASslow0 @00:51:54.69 AS.seek :empty @00:53:35.67 AFV.select :carrierSolution @00:53:35.78 delay 2 seconds @00:53:37.81 AS.reconfigure ASconfig @00:53:37.86 AS.seek 1ml @00:54:35.67 delay 1 second @00:54:36.69 AFV.select :holdingCoil @00:54:36.79 delay 1 second @00:54:37.82 AS.reconfigure ASslow0 @00:54:37.87 AS.seek :empty @00:56:18.83 ATV.dial :air2 @00:56:19.48 AS.reconfigure ASslow1 @00:56:19.53 AS.seek 0.009ml @00:56:21.85 AS.reconfigure ASslow0 @00:56:21.90 AS.seek 1ml @00:58:01.85 ATV.dial AIV @00:58:02.61 AS.seek 0.02ml @00:59:41.51 ATV.dial :waste @00:59:43.09 AS.seek 0ml @00:59:45.40 AWV.select :air @00:59:45.56 Cleaning PCR @00:59:45.58 AFV.select :carrierSolution @00:59:45.68 delay 2 seconds @00:59:47.71 AS.reconfigure ASconfig @00:59:47.76 AS.seek 0.5ml @01:00:16.79 delay 1 second @01:00:17.81 AFV.select :holdingCoil @01:00:17.91 ASV.dial :waste1 @01:00:18.27 ATV.dial :air3 @01:00:18.95 AS.reconfigure ASslow1 @01:00:19.00 AS.seek 0.51ml @01:00:21.27 delay 2 seconds @01:00:23.29 ATV.dial :bleach @01:00:23.76 AS.seek 0.81ml @01:01:24.38 delay 3 seconds @01:01:27.41 ATV.dial :air3 @01:01:27.94 delay 3 seconds @01:01:31.03 AS.seek 0.835ml @01:01:36.36 delay 3 seconds @01:01:39.39 ATV.dial :bleach @01:01:40.00 AS.seek 0.82ml @01:01:43.27 delay 3 seconds @01:01:46.30 ARV.dial :waste @01:01:46.88 delay 3 seconds @01:01:49.91 ATV.dial :ARV @01:01:50.87 AS.reconfigure ASconfig @01:01:50.92 AS.seek 0.47ml @01:02:11.35 AFV.select :carrierSolution @01:02:11.46 delay 2 seconds @01:02:13.50 AS.seek 1ml @01:02:44.29 delay 1 second @01:02:45.31 AFV.select :holdingCoil @01:02:45.41 delay 1 second @01:02:46.44 AS.reconfigure ASfast @01:02:46.49 AS.seek 0ml @01:03:13.85 AFV.select :carrierSolution @01:03:13.96 delay 2 seconds @01:03:15.99 AS.reconfigure ASconfig @01:03:16.04 AS.seek 1ml @01:04:13.82 delay 1 second @01:04:14.84 AFV.select :holdingCoil @01:04:14.94 delay 1 second @01:04:15.97 AS.reconfigure ASfast @01:04:16.02 AS.seek 0ml @01:04:43.37 AFV.select :carrierSolution @01:04:43.48 delay 2 seconds @01:04:45.51 AS.reconfigure ASconfig @01:04:45.56 AS.seek 1ml @01:05:43.37 delay 1 second @01:05:44.39 AFV.select :holdingCoil @01:05:44.49 delay 1 second @01:05:45.52 AS.reconfigure ASfast @01:05:45.57 AS.seek 0ml @01:06:12.93 ARV.dial between carrier and air1 @01:06:13.43 ATV.dial :air3 @01:06:14.67 AS.reconfigure ASslow1 @01:06:14.72 AS.seek 0.01ml @01:06:17.04 delay 2 seconds @01:06:19.06 ATV.dial :bleach @01:06:19.67 AS.seek 0.81ml @01:09:00.79 delay 3 seconds @01:09:03.82 ATV.dial :air3 @01:09:04.36 delay 3 seconds @01:09:08.21 AS.seek 0.835ml @01:09:13.48 delay 3 seconds @01:09:16.51 ATV.dial :bleach @01:09:17.11 AS.seek 0.82ml @01:09:20.42 delay 3 seconds @01:09:23.45 ATV.dial PCR @01:09:23.86 AS.reconfigure ASconfig @01:09:23.91 AS.seek :empty @01:10:11.37 PCR.program :bleachProfile @01:10:11.43 PCR program should complete in < 2:45 @01:10:11.44 PCR.run 10:00 @01:13:40.53 PCR.off @01:13:40.55 AFV.select :carrierSolution @01:13:40.66 delay 2 seconds @01:13:42.70 AS.seek 1ml @01:14:40.53 delay 1 second @01:14:41.55 AFV.select :holdingCoil @01:14:41.65 delay 1 second @01:14:42.68 AS.reconfigure ASfast @01:14:42.73 AS.seek 0ml @01:15:10.09 delay 3 seconds @01:15:13.12 AFV.select :carrierSolution @01:15:13.22 delay 2 seconds @01:15:15.25 AS.reconfigure ASconfig @01:15:15.31 AS.seek 1ml @01:16:13.06 delay 1 second @01:16:14.08 AFV.select :holdingCoil @01:16:14.18 delay 1 second @01:16:15.21 AS.reconfigure ASfast @01:16:15.26 AS.seek 0ml @01:16:42.59 delay 3 seconds @01:16:45.62 AFV.select :carrierSolution @01:16:45.72 delay 2 seconds @01:16:47.75 AS.reconfigure ASconfig @01:16:47.81 AS.seek 1ml @01:17:45.59 delay 1 second @01:17:46.61 AFV.select :holdingCoil @01:17:46.71 delay 1 second @01:17:47.74 AS.reconfigure ASfast @01:17:47.79 AS.seek 0ml @01:18:15.13 delay 3 seconds @01:18:18.16 AFV.select :carrierSolution @01:18:18.26 delay 2 seconds @01:18:20.29 AS.reconfigure ASconfig @01:18:20.35 AS.seek 1ml @01:19:18.10 delay 1 second @01:19:19.12 AFV.select :holdingCoil @01:19:19.22 delay 1 second @01:19:20.25 AS.reconfigure ASfast @01:19:20.30 AS.seek 0ml @01:19:47.64 delay 3 seconds @01:19:50.67 AFV.select :carrierSolution @01:19:50.77 delay 2 seconds @01:19:52.80 AS.reconfigure ASconfig @01:19:52.86 AS.seek 1ml @01:20:50.63 delay 1 second @01:20:51.65 AFV.select :holdingCoil @01:20:51.75 delay 1 second @01:20:52.78 AS.reconfigure ASnorm @01:20:52.83 AS.seek 0ml @01:21:43.55 AFV.select :carrierSolution @01:21:43.66 delay 2 seconds @01:21:45.69 AS.reconfigure ASconfig @01:21:45.74 AS.seek 1ml @01:22:43.54 delay 1 second @01:22:44.56 AFV.select :holdingCoil @01:22:44.66 delay 1 second @01:22:45.69 ATV.dial :waste @01:22:46.85 AS.reconfigure ASnorm @01:22:46.90 AS.seek 0.5ml @01:23:12.47 ATV.dial :waste2 @01:23:13.70 AS.seek 0ml @01:23:39.26 AS.coast @01:23:39.30 SS.coast @01:23:39.33 CC.coast @01:23:39.37 CS.coast @01:23:39.41 PC.coast @01:23:39.45 PS.coast @01:23:39.49 Forearm.coast @01:23:39.53 Elbow.coast @01:23:39.93 Gate.power :core,:OFF @01:23:39.94 Gate.power :raw,:OFF @01:23:39.95 Shutdown @01:23:39.97 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @08:47:29.78 Session Begun @08:48:51.42 -> ESP.wakeup @08:48:51.49 Powering core bus @08:48:56.61 Gate.power :raw,:ON @08:48:57.32 SPE.coast @08:48:57.42 SPE.configure SPEconfig @08:48:57.55 AS.configure ASconfig @08:48:57.69 SS.configure SSconfig @08:48:57.82 CC.configure CCconfig @08:48:57.95 CS.configure CSconfig @08:48:58.08 PC.configure PCconfig @08:48:58.21 PS.configure PSconfig @08:48:58.37 Forearm.configure LooseForearm @08:48:58.51 Elbow.configure LooseElbow @08:48:58.67 SC.configure SCloose @08:48:58.80 SE.configure SEconfig @08:48:58.87 CH.coast @08:48:58.96 CH.configure CHconfig @08:48:59.03 PH.coast @08:48:59.12 PH.configure PHconfig @08:48:59.19 PCR.off @08:48:59.24 PCR.pidtemp= 50,0.1,0,300,25,25 @08:48:59.29 PCR.caltemp= 2.8814,0.0372,0 @08:48:59.34 All dwarf controllers running firmware version 3.75 @08:48:59.48 Valve.reset @08:48:59.68 PCR.off @08:48:59.74 PCR.pidtemp= 50,0.1,0,300,25,25 @08:48:59.79 PCR.caltemp= 2.8814,0.0372,0 @08:48:59.96 Intake.select :close @08:49:00.03 Exhaust.select :close @08:49:00.09 CTV.dial :air @08:49:00.24 PSV.dial :air @08:49:00.33 CSV.dial :waste1 @08:49:00.46 SSV.dial :spit,avoiding: :exhaust @08:49:00.50 Forearm.reconfigure LooseForearm @08:49:00.65 Forearm.home.seek :retract @08:49:00.83 PS.reconfigure PSconfig @08:49:00.91 PS.home.seek 0ml @08:49:01.50 Hand.close @08:49:01.55 Elbow.jog 1500 @08:49:01.63 CS.reconfigure CSfast @08:49:01.76 SS.reconfigure SSconfig @08:49:01.86 CS.home.seek 0ml @08:49:01.91 SS.home.seek 0ml @08:49:02.46 Elbow.reconfigure LooseElbow @08:49:02.53 Elbow.home.seek :clear @08:49:06.05 SE.reconfigure SEconfig @08:49:06.12 SE.home.seek :down @08:49:06.91 SC.reconfigure SCsloppy @08:49:06.98 SC.home.seek :initial @08:49:10.00 PS.coast @08:49:11.06 Forearm.seek :retract @08:49:11.17 Elbow.seek :hammer @08:49:12.46 Forearm.reconfigure TightForearm @08:49:12.54 Forearm.seek 5 + 350 counts @08:49:13.63 Forearm.seek :retract @08:49:14.83 PC.reconfigure PCconfig @08:49:14.86 CC.reconfigure CCconfig @08:49:14.99 PC.home.seek :open @08:49:15.03 CC.home.seek :open @08:49:27.57 -> ATV.ARV @08:49:27.59 ATV.dial ARV @08:52:17.42 -> ARV.air1 @08:52:17.44 ARV.dial :air1 @08:52:29.26 -> AS.pull 0.03 @08:52:29.36 Slide::Error in bkha -- Analytical Syringe Not yet Homed! @08:52:37.58 -> MFB.ready! @08:52:37.68 Readying the MFB @08:52:37.70 Homing MFB Syringe @08:52:37.72 AFV.select :holdingCoil @08:52:37.93 ATV.dial :air2 @08:52:39.11 AS.reconfigure ASconfig @08:52:39.18 AS.home.seek :home @08:52:45.20 AFV.select :carrierSolution @08:52:45.34 delay 2 seconds @08:52:47.38 AS.seek 1ml @08:53:40.32 delay 1 second @08:53:41.34 AFV.select :holdingCoil @08:53:41.47 delay 1 second @08:53:42.50 ATV.dial :waste @08:53:43.53 AS.reconfigure ASnorm @08:53:43.62 AS.home.seek :empty @08:54:36.20 ATV.dial :waste @08:54:36.22 ARV.dial between carrier and air1 @08:54:36.54 ASV.dial :waste1 @08:55:37.29 -> ARV @08:55:50.10 -> ATV.ARV @08:55:50.12 ATV.dial ARV @08:55:54.99 -> ARV.air1 @08:55:55.01 ARV.dial :air1 @08:56:24.52 -> AS.pull 0.03 @08:56:24.63 AS.reconfigure ASconfig @08:56:24.71 AS.seek 0.029ml @08:56:39.24 -> ARV.MM1 @08:56:39.26 ARV.dial :MM1 @08:57:13.84 -> AS.pull 0.05, ASslow1 @08:57:13.95 AS.reconfigure ASslow1 @08:57:14.04 AS.seek 0.079ml @08:57:51.70 -> ARV.air1 @08:57:51.72 ARV.dial :air1 @08:58:20.18 -> AS.pull 0.03, ASslow1 @08:58:20.29 AS.seek 0.109ml @08:58:53.14 -> ARV.carrier @08:58:53.16 ARV.dial :carrier @08:58:56.64 -> ATV @08:59:15.86 -> AS.pull 0.05, ASslow2 @08:59:15.97 AS.reconfigure ASslow2 @08:59:16.04 AS.seek 0.159ml @09:00:47.55 -> ATV.waste @09:00:47.57 ATV.dial :waste @09:00:52.99 -> AS.empty @09:00:53.02 AS.reconfigure ASconfig @09:00:53.13 AS.seek :empty @09:02:04.39 -> pcrDecon @09:02:04.49 Cleaning PCR @09:02:04.50 AFV.select :carrierSolution @09:02:04.66 delay 2 seconds @09:02:06.70 AS.seek 0.5ml @09:02:35.78 delay 1 second @09:02:36.80 AFV.select :holdingCoil @09:02:36.93 ASV.dial :waste1 @09:02:36.95 ATV.dial :air3 @09:02:37.79 AS.reconfigure ASslow1 @09:02:37.86 AS.seek 0.51ml @09:02:40.14 delay 2 seconds @09:02:42.16 ATV.dial :bleach @09:02:42.63 AS.seek 0.81ml @09:03:43.25 delay 3 seconds @09:03:46.28 ATV.dial :air3 @09:03:46.83 delay 3 seconds @09:03:49.92 AS.seek 0.835ml @09:03:55.25 delay 3 seconds @09:03:58.28 ATV.dial :bleach @09:03:58.90 AS.seek 0.82ml @09:04:02.22 delay 3 seconds @09:04:05.25 ARV.dial :waste @09:04:05.66 delay 3 seconds @09:04:08.69 ATV.dial :ARV @09:04:09.59 AS.reconfigure ASconfig @09:04:09.66 AS.seek 0.47ml @09:04:30.08 AFV.select :carrierSolution @09:04:30.21 delay 2 seconds @09:04:32.25 AS.seek 1ml @09:05:03.07 delay 1 second @09:05:04.09 AFV.select :holdingCoil @09:05:04.22 delay 1 second @09:05:05.25 AS.reconfigure ASfast @09:05:05.33 AS.seek 0ml @09:05:32.69 AFV.select :carrierSolution @09:05:32.83 delay 2 seconds @09:05:34.86 AS.reconfigure ASconfig @09:05:34.93 AS.seek 1ml @09:06:32.74 delay 1 second @09:06:33.76 AFV.select :holdingCoil @09:06:33.89 delay 1 second @09:06:34.92 AS.reconfigure ASfast @09:06:35.00 AS.seek 0ml @09:07:02.40 AFV.select :carrierSolution @09:07:02.53 delay 2 seconds @09:07:04.56 AS.reconfigure ASconfig @09:07:04.63 AS.seek 1ml @09:08:02.43 delay 1 second @09:08:03.45 AFV.select :holdingCoil @09:08:03.58 delay 1 second @09:08:04.61 AS.reconfigure ASfast @09:08:04.69 AS.seek 0ml @09:08:32.08 ARV.dial between carrier and air1 @09:08:32.60 ATV.dial :air3 @09:08:33.87 AS.reconfigure ASslow1 @09:08:33.94 AS.seek 0.01ml @09:08:36.27 delay 2 seconds @09:08:38.29 ATV.dial :bleach @09:08:39.62 AS.seek 0.81ml @09:11:20.82 delay 3 seconds @09:11:23.85 ATV.dial :air3 @09:11:24.40 delay 3 seconds @09:11:27.49 AS.seek 0.835ml @09:11:32.80 delay 3 seconds @09:11:35.83 ATV.dial :bleach @09:11:36.45 AS.seek 0.82ml @09:11:39.77 delay 3 seconds @09:11:42.80 ATV.dial PCR @09:11:43.24 AS.reconfigure ASconfig @09:11:43.32 AS.seek :empty @09:12:30.81 PCR.program :bleachProfile @09:12:30.88 PCR program should complete in < 2:45 @09:12:30.90 PCR.run 10:00 @09:16:09.11 PCR.off @09:16:09.16 AFV.select :carrierSolution @09:16:09.30 delay 2 seconds @09:16:11.34 AS.seek 1ml @09:17:09.18 delay 1 second @09:17:10.20 AFV.select :holdingCoil @09:17:10.33 delay 1 second @09:17:11.36 AS.reconfigure ASfast @09:17:11.44 AS.seek 0ml @09:17:38.81 delay 3 seconds @09:17:41.84 AFV.select :carrierSolution @09:17:41.97 delay 2 seconds @09:17:44.00 AS.reconfigure ASconfig @09:17:44.08 AS.seek 1ml @09:18:41.87 delay 1 second @09:18:42.89 AFV.select :holdingCoil @09:18:43.02 delay 1 second @09:18:44.05 AS.reconfigure ASfast @09:18:44.13 AS.seek 0ml @09:19:11.48 delay 3 seconds @09:19:14.51 AFV.select :carrierSolution @09:19:14.64 delay 2 seconds @09:19:16.67 AS.reconfigure ASconfig @09:19:16.75 AS.seek 1ml @09:20:14.54 delay 1 second @09:20:15.56 AFV.select :holdingCoil @09:20:15.69 delay 1 second @09:20:16.72 AS.reconfigure ASfast @09:20:16.80 AS.seek 0ml @09:20:44.17 delay 3 seconds @09:20:47.20 AFV.select :carrierSolution @09:20:47.33 delay 2 seconds @09:20:49.36 AS.reconfigure ASconfig @09:20:49.44 AS.seek 1ml @09:21:47.25 delay 1 second @09:21:48.27 AFV.select :holdingCoil @09:21:48.40 delay 1 second @09:21:49.43 AS.reconfigure ASfast @09:21:49.51 AS.seek 0ml @09:22:16.89 delay 3 seconds @09:22:19.92 AFV.select :carrierSolution @09:22:20.05 delay 2 seconds @09:22:22.08 AS.reconfigure ASconfig @09:22:22.16 AS.seek 1ml @09:23:20.00 delay 1 second @09:23:21.02 AFV.select :holdingCoil @09:23:21.15 delay 1 second @09:23:22.18 AS.reconfigure ASnorm @09:23:22.32 AS.seek 0ml @09:25:14.33 -> ESP.shutdown @09:25:14.36 AS.coast @09:25:14.41 Asleep / Shutdown @09:25:14.50 SS.coast @09:25:14.56 CC.coast @09:25:14.63 CS.coast @09:25:14.69 PC.coast @09:25:14.76 Forearm.coast @09:25:14.82 Elbow.coast @09:25:14.89 SC.coast @09:25:14.95 SE.coast @09:25:15.37 Gate.power :core,:OFF @09:25:15.39 Gate.power :raw,:OFF @09:25:15.42 Shutdown @09:25:22.03 -> exit @09:25:22.05 Session Ended @10:02:09.14
Powering core bus @10:02:14.13 Gate.power :raw,:ON @10:02:14.79 SPE.coast @10:02:14.85 SPE.configure SPEconfig @10:02:14.94 AS.configure ASconfig @10:02:15.04 SS.configure SSconfig @10:02:15.14 CC.configure CCconfig @10:02:15.25 CS.configure CSconfig @10:02:15.35 PC.configure PCconfig @10:02:15.45 PS.configure PSconfig @10:02:15.57 Forearm.configure LooseForearm @10:02:15.67 Elbow.configure LooseElbow @10:02:15.79 SC.configure SCloose @10:02:15.89 SE.configure SEconfig @10:02:15.94 CH.coast @10:02:15.99 CH.configure CHconfig @10:02:16.02 PH.coast @10:02:16.08 PH.configure PHconfig @10:02:16.12 PCR.off @10:02:16.15 PCR.pidtemp= 50,0.1,0,300,25,25 @10:02:16.87 PCR.caltemp= 2.8814,0.0372,0 @10:02:16.90 All dwarf controllers running firmware version 3.75 @10:02:17.01 Valve.reset @10:02:17.18 PCR.off @10:02:17.22 PCR.pidtemp= 50,0.1,0,300,25,25 @10:02:17.25 PCR.caltemp= 2.8814,0.0372,0 @10:02:17.47 Intake.select :close @10:02:17.48 Exhaust.select :close @10:02:17.50 CTV.dial :air @10:02:17.59 PSV.dial :air @10:02:17.63 CSV.dial :waste1 @10:02:17.83 SSV.dial :spit,avoiding: :exhaust @10:02:17.87 PS.reconfigure PSconfig @10:02:17.89
Forearm.reconfigure LooseForearm @10:02:18.02 PS.home.seek 0ml @10:02:18.05
Forearm.home.seek :retract @10:02:18.24 CS.reconfigure CSfast @10:02:18.28 SS.reconfigure SSconfig @10:02:18.37 CS.home.seek 0ml @10:02:18.41 SS.home.seek 0ml @10:02:18.83 Hand.close @10:02:18.86
Elbow.jog 1500 @10:02:19.38 Elbow.reconfigure LooseElbow @10:02:19.43 Elbow.home.seek :clear @10:02:24.22 SE.reconfigure SEconfig @10:02:24.28 SE.home.seek :down @10:02:25.05 SC.reconfigure SCsloppy @10:02:25.10 SC.home.seek :initial @10:02:27.20 PS.coast @10:02:29.30
Forearm.seek :retract @10:02:29.38 Elbow.seek :hammer @10:02:30.58 Forearm.reconfigure TightForearm @10:02:30.63 Forearm.seek 5 + 350 counts @10:02:31.70 Forearm.seek :retract @10:02:32.89 PC.reconfigure PCconfig @10:02:32.90
CC.reconfigure CCconfig @10:02:33.00 PC.home.seek :open @10:02:33.03
CC.home.seek :open @10:02:36.62 Phase [stdphase] @10:02:36.63 GoodNews.email "At 10:02:36 began in tube 3: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:150\n{1 => 22, 2 => 18, 3 => 4}\nCan@10:02:36, 8.7C, 49% humidity, 14.3psia, 13.043V, 0.601A, 0.601A avg, 7.83W",Subject: "Started 20invasivedeployfinal phase stdphase" @10:02:36.65CEST26-May-20
===> STARTED Superphase (phase #16) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @10:02:36.67 Starting initialPurge @10:02:36.71 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:02:36.79 Hand.open @10:02:37.54 Elbow.reconfigure TightElbow @10:02:37.59 Elbow.seek FlushPuck::Garage @10:02:39.68 Elbow.reconfigure LooseElbow @10:02:39.92 Forearm.reconfigure LooseForearm @10:02:40.04 Forearm.seek :garage @10:02:41.02 Hand.close @10:02:41.82 Forearm.seek :retract @10:02:42.79 Elbow.reconfigure TightElbow @10:02:42.84 Elbow.seek CC @10:02:45.90 Elbow.reconfigure LooseElbow @10:02:46.70 Forearm.reconfigure TightForearm @10:02:46.79 Forearm.seek CC @10:02:48.51 CC.seek :closed @10:02:52.89 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.04 @10:02:53.13 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[1B:27->20] @10:02:53.19 Hand.open @10:02:53.99 Forearm.reconfigure LooseForearm @10:02:54.09 Forearm.seek :retract @10:02:55.18 Flushing the Collection Valve::Manifold @10:02:55.49 CSV.dial CSR @10:02:57.08 Pulling 5ml of CSR.flush @10:02:57.10 CSR.select :flush @10:02:57.27 CS.reconfigure CSconfig @10:02:57.39 CS.seek 5ml @10:03:11.14 delay 5 seconds @10:03:16.17 CSR.select :air @10:03:16.47 CS.reconfigure CSfast @10:03:16.57 CS.seek 7.5ml @10:03:23.67 CSV.dial :waste @10:03:25.14 CS.reconfigure CSconfig @10:03:25.29 CS.seek 0ml @10:03:45.49 Purging external Intake @10:03:45.62 CSV.dial :flush @10:03:46.12 CS.reconfigure CSfast @10:03:46.17 CS.seek 10ml @10:04:10.83 delay 5 seconds @10:04:15.86 CSV.dial CBV @10:04:15.90
CBV.dial CSV @10:04:16.72 CTV.dial :intake @10:04:17.54 Intake.select :open @10:04:17.66 CS.seek 0ml @10:04:44.15 Intake.select :close @10:04:44.26 CTV.dial :air @10:04:45.09 Flushing upper manifold @10:04:45.22 CSV.dial :flush @10:04:46.15 CS.seek 5ml @10:04:59.09 delay 5 seconds @10:05:04.12 CSV.dial :air @10:05:04.69 CS.seek 7ml @10:05:10.52 CSV.dial CBV @10:05:10.55
CBV.dial CSV @10:05:11.68 CTV.dial :air @10:05:11.70 CS.seek 0ml @10:05:29.18 delay 7 seconds @10:05:36.22 CS.seek 3ml @10:05:44.27 CSV.dial :waste @10:05:44.77 CS.seek 0ml @10:05:53.05 CSV.dial :flush @10:05:53.53 CS.seek 3ml @10:06:01.71 delay 5 seconds @10:06:06.74 CSV.dial :air @10:06:07.42 CS.seek 5ml @10:06:13.21 CSV.dial :air @10:06:13.23 CS.seek 0ml @10:06:26.02 delay 7 seconds @10:06:33.06 CS.seek 3ml @10:06:41.09 CSV.dial :waste @10:06:41.89 CS.seek 0ml @10:06:50.02 Garaging FlushPuck in Collection Clamp @10:06:50.10 Elbow.reconfigure TightElbow @10:06:50.15 Elbow.seek CC @10:06:50.42 Elbow.reconfigure LooseElbow @10:06:50.53 Forearm.seek CC @10:06:51.53 Hand.close @10:06:52.17 CC.seek :open @10:06:56.53 Forearm.seek :retract @10:06:57.59 Elbow.reconfigure TightElbow @10:06:57.64 Elbow.seek :garage @10:07:00.79 Elbow.reconfigure LooseElbow @10:07:00.89 Forearm.reconfigure TightForearm @10:07:00.94 Forearm.seek :garage @10:07:02.09 Hand.open @10:07:02.74 Forearm.reconfigure LooseForearm @10:07:02.79 Forearm.seek :retract @10:07:03.92 Starting mfb @10:07:03.94 pcrBAC sampling at most 1500ml, wcr at most 1500ml @10:07:03.97 BACfans.email "Started mfb in tube 3 at 10:07:03CEST26-May-20\n{1 => 22, 2 => 18, 3 => 4}\nCan@10:06:09, 8.7C, 50% humidity, 14.3psia, 13.019V, 0.613A, 0.607A avg, 7.98W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @10:07:04.06
Loading sh1 puck from tube 3 into Collection Clamp @10:07:04.18 Readying the MFB @10:07:04.24 Homing MFB Syringe @10:07:04.25 AFV.select :holdingCoil @10:07:04.53 ATV.dial :air2 @10:07:04.55
SC.reconfigure SCtight @10:07:04.65 SC.seek 3 @10:07:06.03 AS.reconfigure ASconfig @10:07:06.14 AS.home.seek :home @10:07:06.85
SC.reconfigure SCloose @10:07:07.09 Elbow.reconfigure TightElbow @10:07:07.18 Elbow.seek 3 @10:07:10.09 Forearm.reconfigure TightForearm @10:07:10.14 Forearm.seek 3 + 700 counts @10:07:11.42 SE.reconfigure SEpick @10:07:11.55 SE.seek beamBreak @10:07:11.76 AFV.select :carrierSolution @10:07:11.90 delay 2 seconds @10:07:13.96 AS.seek 1ml @10:07:14.66
SE.configure SEconfig @10:07:15.05 Elbow.seek 3 @10:07:15.67 Forearm.seek 3 + 300 counts @10:07:15.96 Forearm.reconfigure LooseForearm @10:07:16.06 Hand.close @10:07:16.75 SE.seek :down @10:07:17.30
Forearm.seek :retract @10:07:18.34 Elbow.seek CC @10:07:19.45 SE.coast @10:07:20.18
Elbow.reconfigure LooseElbow @10:07:20.47 Forearm.reconfigure TightForearm @10:07:20.56 Forearm.seek CC @10:07:22.26 CC.seek :closed @10:07:26.75 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.31 @10:07:26.79 Hand.open @10:07:27.71 Forearm.reconfigure LooseForearm @10:07:27.81 Forearm.seek :retract @10:07:28.91 SC.coast @10:07:28.99 collectSample 1500ml w/bubble point of 55psi @10:07:29.02 Priming 7ml @10:07:29.04 Intake.select :close @10:07:29.06 Exhaust.select :close @10:07:29.09 SS.hold @10:07:29.59 Calibrating pressure and temperature sensors @10:07:29.63 CTV.dial :air @10:07:29.65 SSV.dial :spit,avoiding: :exhaust @10:07:29.73 SS.reconfigure SSfast @10:07:29.83 SS.seek 5ml @10:07:32.39 SSV.dial CBV @10:07:32.46
CBV.dial SSV @10:07:34.03 delay 3.8 seconds @10:07:38.12 SSV.dial :spit @10:07:39.61 SS.seek 0ml @10:07:42.28 SSV.dial CBV @10:07:43.77 CTV.dial :intake @10:07:44.70 SSV.dial CBV @10:07:44.75
CBV.dial SSV @10:07:44.79 Allowing 40s for 7.0ml of remaining 7.0ml @10:07:44.81 Intake.select :open @10:07:47.12 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:07:47.15 SS.reconfigure SSinhale.with maxSpeed: 150 @10:07:47.26 SS.seek 7ml @10:07:50.93 Vacuum too high -- waiting up to 40s @10:07:53.79 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:07:53.83 SS.reconfigure SSinhale.with maxSpeed: 135 @10:07:53.96 SS.seek 7ml @10:07:57.37 Vacuum too high -- waiting up to 40s @10:07:59.60 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:07:59.64 SS.reconfigure SSinhale.with maxSpeed: 122 @10:07:59.74 SS.seek 7ml @10:07:59.99 Stroke completed -- waiting for pressure to equalize ... @10:08:03.35 Intake.select :close @10:08:03.59 SSV.dial :exhaust @10:08:04.47 Exhaust.select :open @10:08:04.65 SS.reconfigure SSexhale @10:08:04.74 SS.seek 0ml @10:08:06.92 delay 1 second @10:08:07.95 AFV.select :holdingCoil @10:08:08.08 delay 1 second @10:08:08.77
Exhaust.select :close @10:08:08.93 Sampling 1500.0ml @10:08:08.96 Intake.select :close @10:08:08.98 Exhaust.select :close @10:08:09.01 SS.reconfigure SSconfig @10:08:09.09 SS.hold @10:08:09.31 ATV.dial :waste @10:08:09.63
CTV.dial :intake @10:08:09.67 SSV.dial CBV @10:08:09.72
CBV.dial SSV @10:08:10.38 AS.reconfigure ASnorm @10:08:10.48 AS.home.seek :empty @10:08:10.66
Allowing 130s for 25.0ml of remaining 1500.0ml @10:08:10.69 Intake.select :open @10:08:12.97 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:08:13.01 SS.reconfigure SSinhale.with maxSpeed: 122 @10:08:13.11 SS.seek 25ml @10:08:16.46 Vacuum too high -- waiting up to 40s @10:08:18.56 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:08:18.59 SS.reconfigure SSinhale.with maxSpeed: 110 @10:08:18.69 SS.seek 25ml @10:08:21.71 Vacuum too high -- waiting up to 40s @10:08:23.81 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:08:23.83 SS.reconfigure SSinhale.with maxSpeed: 99 @10:08:23.88 SS.seek 25ml @10:08:26.67 Vacuum too high -- waiting up to 40s @10:08:29.43 SS.reconfigure SSinhale.with maxSpeed: 89 @10:08:29.50 SS.seek 25ml @10:08:32.82 Vacuum too high -- waiting up to 40s @10:08:34.69 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:08:34.70 SS.reconfigure SSinhale.with maxSpeed: 80 @10:08:34.76 SS.seek 25ml @10:08:37.42 Vacuum too high -- waiting up to 40s @10:08:39.28 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:08:39.30 SS.reconfigure SSinhale.with maxSpeed: 72 @10:08:39.35 SS.seek 25ml @10:08:42.17 Vacuum too high -- waiting up to 40s @10:08:44.04 SS.reconfigure SSinhale.with maxSpeed: 65 @10:08:44.09 SS.seek 25ml @10:08:47.04 Vacuum too high -- waiting up to 40s @10:08:48.88 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:08:48.90 SS.reconfigure SSinhale.with maxSpeed: 59 @10:08:48.95 SS.seek 25ml @10:08:52.79 Vacuum too high -- waiting up to 40s @10:08:54.63 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:08:54.64 SS.reconfigure SSinhale.with maxSpeed: 53 @10:08:54.69 SS.seek 25ml @10:08:58.72 Vacuum too high -- waiting up to 40s @10:09:00.54 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:09:00.56 SS.reconfigure SSinhale.with maxSpeed: 48 @10:09:00.60 SS.seek 25ml @10:09:01.61 Stroke completed -- waiting for pressure to equalize ... @10:09:03.06 ATV.dial :waste @10:09:03.08 ARV.dial between carrier and air1 @10:09:03.17 ASV.dial :waste1 @10:09:04.93
Intake.select :close @10:09:05.09 SSV.dial :exhaust @10:09:05.92 Exhaust.select :open @10:09:06.04 SS.reconfigure SSexhale @10:09:06.08 SS.seek 0ml @10:09:15.12 Exhaust.select :close @10:09:15.24 SSV.dial CBV @10:09:15.26
CBV.dial SSV @10:09:16.11 Allowing 130s for 25.0ml of remaining 1475.0ml @10:09:16.12 Intake.select :open @10:09:18.31 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:09:18.32 SS.reconfigure SSinhale.with maxSpeed: 48 @10:09:18.37 SS.seek 25ml @10:09:24.39 Vacuum too high -- waiting up to 40s @10:09:25.97 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:09:25.98 SS.reconfigure SSinhale.with maxSpeed: 43 @10:09:26.04 SS.seek 25ml @10:09:31.42 Vacuum too high -- waiting up to 40s @10:09:33.49 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:09:33.51 SS.reconfigure SSinhale.with maxSpeed: 39 @10:09:33.56 SS.seek 25ml @10:09:39.77 Vacuum too high -- waiting up to 40s @10:09:41.83 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:09:41.85 SS.reconfigure SSinhale.with maxSpeed: 35 @10:09:41.89 SS.seek 25ml @10:09:48.33 Vacuum too high -- waiting up to 40s @10:09:50.14 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:09:50.16 SS.reconfigure SSinhale.with maxSpeed: 32 @10:09:50.20 SS.seek 25ml @10:09:57.48 Vacuum too high -- waiting up to 40s @10:09:59.84 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:09:59.85 SS.reconfigure SSinhale.with maxSpeed: 29 @10:09:59.90 SS.seek 25ml @10:10:09.14 Vacuum too high -- waiting up to 40s @10:10:11.25 SS.reconfigure SSinhale.with maxSpeed: 26 @10:10:11.30 SS.seek 25ml @10:10:23.96 Vacuum too high -- waiting up to 40s @10:10:26.29 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:10:26.30 SS.reconfigure SSinhale.with maxSpeed: 23 @10:10:26.35 SS.seek 25ml @10:10:29.01 Stroke completed -- waiting for pressure to equalize ... @10:10:34.69 Intake.select :close @10:10:34.85 SSV.dial :exhaust @10:10:35.67 Exhaust.select :open @10:10:35.79 SS.reconfigure SSexhale @10:10:35.83 SS.seek 0ml @10:10:44.92 Exhaust.select :close @10:10:45.04 SSV.dial CBV @10:10:45.06
CBV.dial SSV @10:10:45.90 Allowing 130s for 25.0ml of remaining 1450.0ml @10:10:45.91 Intake.select :open @10:10:48.09 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:10:48.11 SS.reconfigure SSinhale.with maxSpeed: 23 @10:10:48.16 SS.seek 25ml @10:11:15.42 Vacuum too high -- waiting up to 40s @10:11:17.49 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:11:17.50 SS.reconfigure SSinhale.with maxSpeed: 21 @10:11:17.55 SS.seek 25ml @10:12:08.14 Vacuum too high -- waiting up to 40s @10:12:10.81 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:12:10.83 SS.reconfigure SSinhale.with maxSpeed: 19 @10:12:10.87 SS.seek 25ml @10:12:14.69 Stroke completed -- waiting for pressure to equalize ... @10:12:21.05 Intake.select :close @10:12:21.21 SSV.dial :exhaust @10:12:22.04 Exhaust.select :open @10:12:22.16 SS.reconfigure SSexhale @10:12:22.20 SS.seek 0ml @10:12:31.75 Exhaust.select :close @10:12:31.87 SSV.dial CBV @10:12:31.89
CBV.dial SSV @10:12:32.75 Allowing 130s for 25.0ml of remaining 1425.0ml @10:12:32.76 Intake.select :open @10:12:34.93 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:12:34.95 SS.reconfigure SSinhale.with maxSpeed: 19 @10:12:35.00 SS.seek 25ml @10:14:07.12 Stroke completed -- waiting for pressure to equalize ... @10:14:14.66 Intake.select :close @10:14:14.81 SSV.dial :exhaust @10:14:15.62 Exhaust.select :open @10:14:15.74 SS.reconfigure SSexhale @10:14:15.78 SS.seek 0ml @10:14:25.08 Exhaust.select :close @10:14:25.20 SSV.dial CBV @10:14:25.22
CBV.dial SSV @10:14:26.08 Allowing 130s for 25.0ml of remaining 1400.0ml @10:14:26.09 Intake.select :open @10:14:28.26 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:14:28.28 SS.reconfigure SSinhale.with maxSpeed: 19 @10:14:28.33 SS.seek 25ml @10:16:00.53 Stroke completed -- waiting for pressure to equalize ... @10:16:08.13 Intake.select :close @10:16:08.29 SSV.dial :exhaust @10:16:09.12 Exhaust.select :open @10:16:09.24 SS.reconfigure SSexhale @10:16:09.28 SS.seek 0ml @10:16:18.53 Exhaust.select :close @10:16:18.65 SSV.dial CBV @10:16:18.67
CBV.dial SSV @10:16:19.51 Allowing 130s for 25.0ml of remaining 1375.0ml @10:16:19.52 Intake.select :open @10:16:21.70 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:16:21.71 SS.reconfigure SSinhale.with maxSpeed: 19 @10:16:21.76 SS.seek 25ml @10:17:53.86 Stroke completed -- waiting for pressure to equalize ... @10:18:00.85 Intake.select :close @10:18:01.01 SSV.dial :exhaust @10:18:01.83 Exhaust.select :open @10:18:01.95 SS.reconfigure SSexhale @10:18:01.99 SS.seek 0ml @10:18:11.00 Exhaust.select :close @10:18:11.12 SSV.dial CBV @10:18:11.14
CBV.dial SSV @10:18:11.98 Allowing 130s for 25.0ml of remaining 1350.0ml @10:18:11.99 Intake.select :open @10:18:14.19 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:18:14.20 SS.reconfigure SSinhale.with maxSpeed: 19 @10:18:14.25 SS.seek 25ml @10:19:46.62 Stroke completed -- waiting for pressure to equalize ... @10:19:53.56 Intake.select :close @10:19:53.72 SSV.dial :exhaust @10:19:54.56 Exhaust.select :open @10:19:54.68 SS.reconfigure SSexhale @10:19:54.72 SS.seek 0ml @10:20:03.75 Exhaust.select :close @10:20:03.87 SSV.dial CBV @10:20:03.89
CBV.dial SSV @10:20:04.75 Allowing 130s for 25.0ml of remaining 1325.0ml @10:20:04.76 Intake.select :open @10:20:06.93 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:20:06.95 SS.reconfigure SSinhale.with maxSpeed: 19 @10:20:06.99 SS.seek 25ml @10:21:39.37 Stroke completed -- waiting for pressure to equalize ... @10:21:46.98 Intake.select :close @10:21:47.14 SSV.dial :exhaust @10:21:47.97 Exhaust.select :open @10:21:48.09 SS.reconfigure SSexhale @10:21:48.13 SS.seek 0ml @10:21:57.48 Exhaust.select :close @10:21:57.59 SSV.dial CBV @10:21:57.63
CBV.dial SSV @10:21:58.47 Allowing 130s for 25.0ml of remaining 1300.0ml @10:21:58.48 Intake.select :open @10:22:00.66 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:22:00.67 SS.reconfigure SSinhale.with maxSpeed: 19 @10:22:00.72 SS.seek 25ml @10:23:33.06 Stroke completed -- waiting for pressure to equalize ... @10:23:41.28 Intake.select :close @10:23:41.44 SSV.dial :exhaust @10:23:42.27 Exhaust.select :open @10:23:42.39 SS.reconfigure SSexhale @10:23:42.43 SS.seek 0ml @10:23:51.45 Exhaust.select :close @10:23:51.57 SSV.dial CBV @10:23:51.59
CBV.dial SSV @10:23:52.44 Allowing 130s for 25.0ml of remaining 1275.0ml @10:23:52.45 Intake.select :open @10:23:54.62 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:23:54.64 SS.reconfigure SSinhale.with maxSpeed: 19 @10:23:54.69 SS.seek 25ml @10:25:27.12 Stroke completed -- waiting for pressure to equalize ... @10:25:36.53 Intake.select :close @10:25:36.69 SSV.dial :exhaust @10:25:37.52 Exhaust.select :open @10:25:37.64 SS.reconfigure SSexhale @10:25:37.69 SS.seek 0ml @10:25:47.05 Exhaust.select :close @10:25:47.17 SSV.dial CBV @10:25:47.19
CBV.dial SSV @10:25:48.03 Allowing 130s for 25.0ml of remaining 1250.0ml @10:25:48.04 Intake.select :open @10:25:50.21 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:25:50.23 SS.reconfigure SSinhale.with maxSpeed: 19 @10:25:50.28 SS.seek 25ml @10:27:14.61 Vacuum too high -- waiting up to 40s @10:27:16.70 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:27:16.72 SS.reconfigure SSinhale.with maxSpeed: 17 @10:27:16.77 SS.seek 25ml @10:27:27.04 Stroke completed -- waiting for pressure to equalize ... @10:27:35.27 Intake.select :close @10:27:35.42 SSV.dial :exhaust @10:27:36.24 Exhaust.select :open @10:27:36.36 SS.reconfigure SSexhale @10:27:36.40 SS.seek 0ml @10:27:45.46 Exhaust.select :close @10:27:45.58 SSV.dial CBV @10:27:45.60
CBV.dial SSV @10:27:46.46 Allowing 130s for 25.0ml of remaining 1225.0ml @10:27:46.47 Intake.select :open @10:27:48.65 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:27:48.66 SS.reconfigure SSinhale.with maxSpeed: 17 @10:27:48.71 SS.seek 25ml @10:29:32.04 Stroke completed -- waiting for pressure to equalize ... @10:29:43.35 Intake.select :close @10:29:43.50 SSV.dial :exhaust @10:29:44.33 Exhaust.select :open @10:29:44.45 SS.reconfigure SSexhale @10:29:44.49 SS.seek 0ml @10:29:53.85 Exhaust.select :close @10:29:53.97 SSV.dial CBV @10:29:53.99
CBV.dial SSV @10:29:54.85 Allowing 130s for 25.0ml of remaining 1200.0ml @10:29:54.86 Intake.select :open @10:29:57.62 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:29:57.74 SS.reconfigure SSinhale.with maxSpeed: 17 @10:29:57.79 SS.seek 25ml @10:31:05.40 Vacuum too high -- waiting up to 40s @10:31:08.24 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:31:08.26 SS.reconfigure SSinhale.with maxSpeed: 15 @10:31:08.31 SS.seek 25ml @10:31:49.04 Stroke completed -- waiting for pressure to equalize ... @10:32:00.36 Intake.select :close @10:32:00.52 SSV.dial :exhaust @10:32:01.33 Exhaust.select :open @10:32:01.45 SS.reconfigure SSexhale @10:32:01.50 SS.seek 0ml @10:32:10.94 Exhaust.select :close @10:32:11.06 SSV.dial CBV @10:32:11.08
CBV.dial SSV @10:32:11.93 Allowing 130s for 25.0ml of remaining 1175.0ml @10:32:11.94 Intake.select :open @10:32:14.11 Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:32:14.13 SS.reconfigure SSinhale.with maxSpeed: 15 @10:32:14.18 SS.seek 25ml @10:34:11.17 Stroke completed -- waiting for pressure to equalize ... @10:34:21.96 SS.reconfigure SSconfig @10:34:22.01 SS.hold @10:34:22.14 Intake.select :close @10:34:22.30 ShallowSampler::Clogged in MAIN -- progress too slow after 350.0ml @10:34:22.32 SSV.dial :exhaust @10:34:23.16 Exhaust.select :open @10:34:23.28 SS.reconfigure SSexhale @10:34:23.32 SS.seek 0ml @10:34:32.55 Exhaust.select :close @10:34:32.66 Sampled 350.0ml @10:34:32.68 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:34:32.69 Intake.select :close @10:34:32.76 CSV.dial :waste @10:34:32.84 CS.seek 0ml @10:34:32.92 SSV.dial CBV,avoiding: :exhaust @10:34:32.94
CBV.dial SSV @10:34:33.15 CSV.dial :air @10:34:33.96 CS.seek 10ml @10:34:35.02
CTV.dial :air @10:34:35.85 delay 15 seconds @10:34:50.88 SSV.dial :spit,avoiding: :exhaust @10:34:52.34 CTV.dial CSV,avoiding: :intake @10:34:52.38
SS.seek 0ml @10:34:52.53 SSV.dial CBV,avoiding: :exhaust @10:34:59.35 CBV.dial between SSV and waste2 @10:34:59.78 CTV.dial CSV,avoiding: :air @10:34:59.80
CSV.dial CTV @10:35:00.30 CS.reconfigure CSpressurize @10:35:00.34 CS.seek 0ml @10:35:26.21 BubblePoint: Intake @70.2psia, Outlet @14.3psia @10:35:26.22 CBV.dial SSV @10:35:26.57 SS.reconfigure SSconfig @10:35:26.63 SS.seek 10ml @10:35:32.00 delay 15 seconds @10:35:47.15 Evacuated: Intake @36.5psia, Outlet @0.7psia @10:35:47.16 SSV.dial :spit,avoiding: :exhaust @10:35:48.62 SS.reconfigure SSexhale @10:35:48.66 SS.seek 0ml @10:35:53.56 SS.reconfigure SSfast @10:35:53.61 SS.seek 5ml @10:35:56.50 SSV.dial CBV,avoiding: :exhaust @10:35:56.53
CBV.dial SSV @10:35:57.97 CTV.dial :air,avoiding: :intake @10:35:59.44 CSV.dial CTV @10:35:59.48
SS.seek 10ml @10:36:00.48 CSV.dial :air @10:36:00.97 CS.reconfigure CSfast @10:36:01.02 CS.seek 0ml @10:36:02.09 CS.coast @10:36:02.15
SSV.dial :spit,avoiding: :exhaust @10:36:03.60 SS.reconfigure SSexhale @10:36:03.65 SS.seek 0ml @10:36:08.66 SS.reconfigure SSfast @10:36:08.71 SS.seek 5ml @10:36:11.68 SSV.dial CBV,avoiding: :exhaust @10:36:11.73
CBV.dial SSV @10:36:14.20 SSV.dial :spit,avoiding: :exhaust @10:36:15.63 SS.reconfigure SSconfig @10:36:15.68 SS.seek 0ml @10:36:18.68 SS.coast @10:36:18.72 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:36:18.73 Intake.select :close @10:36:18.81 CSV.dial :waste @10:36:18.96 SSV.dial CBV,avoiding: :exhaust @10:36:19.66
CBV.dial SSV @10:36:19.76 CS.seek 0ml @10:36:19.84 CSV.dial :air @10:36:20.65 CS.seek 10ml @10:36:21.11
CTV.dial :air @10:36:21.13 delay 15 seconds @10:36:36.15 SSV.dial :spit,avoiding: :exhaust @10:36:37.59 CTV.dial CSV,avoiding: :intake @10:36:37.63
SS.reconfigure SSexhale @10:36:37.74 SS.seek 0ml @10:36:38.02 SSV.dial CBV,avoiding: :exhaust @10:36:45.63 CBV.dial between SSV and waste2 @10:36:46.05 CSV.dial between air and CTV @10:36:46.38 CS.seek 8.211ml @10:36:51.66 CTV.dial CSV,avoiding: :air @10:36:51.68
CSV.dial CTV @10:36:52.15 CS.reconfigure CSpressurize @10:36:52.19 CS.seek 0ml @10:37:13.75 CSV.dial :air @10:37:14.26 CS.reconfigure CSfast @10:37:14.30 CS.seek 10ml @10:37:39.00 CSV.dial between air and CTV @10:37:39.37 CS.seek 3.039ml @10:37:57.04 CTV.dial CSV,avoiding: :air @10:37:57.06
CSV.dial CTV @10:37:57.40 CS.reconfigure CSpressurize @10:37:57.44 CS.seek 0ml @10:38:04.68 BubblePoint: Intake @69.2psia, Outlet @13.8psia @10:38:04.69 CBV.dial SSV @10:38:05.05 SS.reconfigure SSconfig @10:38:05.10 SS.seek 10ml @10:38:10.60 delay 15 seconds @10:38:25.74 Evacuated: Intake @39.8psia, Outlet @15.0psia @10:38:25.75 SSV.dial :spit,avoiding: :exhaust @10:38:27.19 SS.reconfigure SSexhale @10:38:27.24 SS.seek 0ml @10:38:32.23 SS.reconfigure SSfast @10:38:32.28 SS.seek 5ml @10:38:34.99 SSV.dial CBV,avoiding: :exhaust @10:38:35.02
CBV.dial SSV @10:38:36.47 CTV.dial :air,avoiding: :intake @10:38:37.94 CSV.dial CTV @10:38:37.98
SS.seek 10ml @10:38:38.98 CSV.dial :air @10:38:39.48 CS.reconfigure CSfast @10:38:39.53 CS.seek 0ml @10:38:40.66
SSV.dial :spit,avoiding: :exhaust @10:38:41.62 CS.coast @10:38:42.10
SS.reconfigure SSexhale @10:38:42.15 SS.seek 0ml @10:38:47.11 SS.reconfigure SSfast @10:38:47.16 SS.seek 5ml @10:38:49.88 SSV.dial CBV,avoiding: :exhaust @10:38:49.91
CBV.dial SSV @10:38:52.34 SSV.dial :spit,avoiding: :exhaust @10:38:53.79 SS.reconfigure SSconfig @10:38:53.84 SS.seek 0ml @10:38:57.56 SS.coast @10:38:57.69 Lysing: Adding 1.5ml lysis @10:38:57.70 CBV.dial between CSV and waste1,avoiding: :DBV @10:38:59.35 CTV.dial CSV @10:39:00.80 CSV.dial CSR @10:39:01.67 Pulling 1ml of CSR.lysis @10:39:01.68 CSR.select :lysis @10:39:01.80 CS.reconfigure CSconfig @10:39:01.84 CS.seek 1ml @10:39:05.35 delay 5 seconds @10:39:10.38 CSR.select :air @10:39:10.56 CS.seek 6ml @10:39:24.28 delay 2 seconds @10:39:26.30 CSV.dial :waste1 @10:39:27.72 CS.reconfigure CSfast @10:39:27.77 CS.seek :empty @10:39:42.94 CSV.dial CSR @10:39:44.41 Pulling 1.5ml of CSR.lysis @10:39:44.42 CSR.select :lysis @10:39:44.54 CS.reconfigure CSconfig @10:39:44.58 CS.seek 1.5ml @10:39:49.53 delay 5 seconds @10:39:54.56 CSR.select :air @10:39:54.74 CS.seek 4.501ml @10:40:03.38 delay 2 seconds @10:40:05.40 CSV.dial CTV @10:40:05.90 CS.seek 1.275ml @10:40:15.23 Heating sh1 puck from tube 3 in Collection Clamp to 85C for 5:00 @10:40:15.25 CH.seek 85C,8:00 @10:43:39.68 CH.hold 5:00,85C @10:48:39.85 Collection Puck Heater at 85.1C after lysing @10:48:39.87 CS.seek 0ml @10:48:44.33 CTV.dial between spare and air,avoiding: :intake @10:48:45.48 CSV.dial :air @10:48:45.99 CS.seek 5ml @10:48:59.79 CSV.dial CTV @10:49:00.28 delay 2 seconds @10:49:02.30 CSV.dial :air @10:49:02.81 delay 2 seconds @10:49:04.83 CSV.dial CTV @10:49:05.33 delay 2 seconds @10:49:07.35 CSV.dial :air @10:49:07.97 delay 2 seconds @10:49:10.00 CS.seek 0ml @10:49:23.70 Recovering Lysate into Collection syringe @10:49:23.71 CSV.dial CBV @10:49:24.84 CBV.dial CSV @10:49:25.24 CS.seek 5ml @10:49:38.98 delay 5 seconds @10:49:44.01 CTV.dial :air @10:49:44.58 CS.seek 7ml @10:49:50.73 delay 5 seconds @10:49:55.76 CSV.dial :air2 @10:49:56.26 CS.seek 10ml @10:50:04.86 CSV.dial between air and CTV,avoiding: CBV @10:50:06.12 Lysing Complete @10:50:06.20 Storing sh1 puck in Collection Clamp to tube 2 @10:50:06.39 SC.reconfigure SCtight @10:50:06.44 SC.seek 2 @10:50:08.64 SC.reconfigure SCloose @10:50:08.86 Elbow.reconfigure TightElbow @10:50:08.91 Elbow.seek 2 @10:50:10.80 Forearm.reconfigure TightForearm @10:50:10.85 Forearm.seek 2 + 700 counts @10:50:11.85 SE.reconfigure SEpick @10:50:11.93 SE.seek beamBreak @10:50:13.36 SE.configure SEconfig @10:50:13.53 Elbow.reconfigure LimpElbow @10:50:13.58 Forearm.reconfigure LooseForearm @10:50:13.62 Forearm.seek 2 @10:50:13.90 Elbow.hold @10:50:13.97 Elbow.reconfigure TightElbow @10:50:14.07 SE.seek 17.09 Pucks below Top Plate @10:50:14.64 Forearm.force -20 @10:50:14.68 Forearm.seek 2 + 342 counts @10:50:15.59 Forearm.reconfigure TightForearm @10:50:15.74 Forearm.seek 2 + 700 counts @10:50:16.16 SE.seek 18.09 Pucks below Top Plate @10:50:16.86 Forearm.reconfigure LooseForearm @10:50:16.91 Forearm.seek :retract @10:50:17.84 Elbow.seek CC @10:50:19.55 Elbow.reconfigure LooseElbow @10:50:19.66 Forearm.seek CC @10:50:20.73 Hand.close @10:50:21.50 CC.seek :open @10:50:25.99 Forearm.seek :retract @10:50:27.03 Elbow.reconfigure TightElbow @10:50:27.08 Elbow.seek 2 @10:50:29.11 Elbow.reconfigure LimpElbow @10:50:29.17 Forearm.seek 2 @10:50:30.15 Elbow.hold @10:50:30.22 Elbow.reconfigure TightElbow @10:50:30.27 Hand.open @10:50:31.14 Forearm.force -20 @10:50:31.18 Forearm.seek 2 + 349 counts @10:50:31.50 Forearm.reconfigure TightForearm @10:50:31.55 Forearm.seek 2 + 700 counts @10:50:31.95 SE.seek :down @10:50:33.03 SE.coast @10:50:33.19
Forearm.reconfigure LooseForearm @10:50:33.24 Forearm.seek :retract @10:50:34.15 SC.coast @10:50:34.19 Tranferring lysate to PRV @10:50:34.20 CSV.dial :air @10:50:34.53 CS.seek 10ml @10:50:34.60 CSV.dial between air and CTV @10:50:34.94 AIV.dial :bypass @10:50:35.03 Beginning samplePassC @10:50:35.05 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:50:35.12 Hand.open @10:50:35.51 Elbow.seek FlushPuck::Garage @10:50:37.51 Elbow.reconfigure LooseElbow @10:50:37.62 Forearm.seek :garage @10:50:38.59 Hand.close @10:50:39.38 Forearm.seek :retract @10:50:40.36 Elbow.reconfigure TightElbow @10:50:40.41 Elbow.seek CC @10:50:43.34 Elbow.reconfigure LooseElbow @10:50:43.44 Forearm.reconfigure TightForearm @10:50:43.49 Forearm.seek CC @10:50:45.58 CC.seek :closed @10:50:49.86 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.16 @10:50:49.94 Hand.open @10:50:50.69 Forearm.reconfigure LooseForearm @10:50:50.74 Forearm.seek :retract @10:50:51.75 AWV.select :air @10:50:51.76 AIV.dial :bypass @10:50:51.77 PSV.dial PSR @10:50:53.23 Pulling 1.5ml of PSR.flush @10:50:53.24 PSR.select :flush @10:50:53.37 PS.seek 1.5ml @10:51:09.46 delay 5 seconds @10:51:14.49 PSV.dial :waste1 @10:51:15.01 PS.seek 0ml @10:51:31.09 PSV.dial PSR @10:51:31.57 PSR.select :air @10:51:31.69 PS.seek 2.5ml @10:51:57.81 PRV.dial CBV @10:51:57.84
CBV.dial PRV @10:51:58.74 PSV.dial :mixloop @10:51:59.84 CTV.dial :air,avoiding: :intake @10:51:59.85 PTV.dial :PRVmixing @10:51:59.94 PS.seek 0ml @10:52:26.09 PSV.dial :air @10:52:26.58 PS.seek 2.5ml @10:52:52.75 PSV.dial :mixloop @10:52:53.26 PS.seek 0ml @10:53:19.43 PSV.dial :air @10:53:19.93 PS.seek 2.5ml @10:53:46.00 PSV.dial :mixloop @10:53:46.49 PS.seek 0ml @10:54:12.69 Garaging FlushPuck in Collection Clamp @10:54:12.77 Elbow.reconfigure TightElbow @10:54:12.82 Elbow.seek CC @10:54:13.08 Elbow.reconfigure LooseElbow @10:54:13.19 Forearm.seek CC @10:54:14.24 Hand.close @10:54:14.88 CC.seek :open @10:54:19.24 Forearm.seek :retract @10:54:20.30 Elbow.reconfigure TightElbow @10:54:20.35 Elbow.seek :garage @10:54:23.59 Elbow.reconfigure LooseElbow @10:54:23.70 Forearm.reconfigure TightForearm @10:54:23.75 Forearm.seek :garage @10:54:24.93 Hand.open @10:54:25.57 Forearm.reconfigure LooseForearm @10:54:25.62 Forearm.seek :retract @10:54:26.66 Connecting PS to CS to transfer sample @10:54:26.67 CBV.dial AIV @10:54:26.68 AIV.dial :bypass @10:54:26.69 PSV.dial :mixloop @10:54:26.71 PTV.dial :PRVmixing @10:54:26.72 PRV.dial AIV @10:54:26.73 CTV.dial :air,avoiding: :intake @10:54:26.75 Loading lyfil puck from tube 3 into Collection Clamp @10:54:27.70 SC.reconfigure SCtight @10:54:27.75 SC.seek 3 @10:54:29.97 SC.reconfigure SCloose @10:54:30.20 Elbow.reconfigure TightElbow @10:54:30.25 Elbow.seek 3 @10:54:32.31 Forearm.reconfigure TightForearm @10:54:32.36 Forearm.seek 3 + 700 counts @10:54:33.40 SE.reconfigure SEpick @10:54:33.48 SE.seek beamBreak @10:54:36.43 SE.configure SEconfig @10:54:36.60 Elbow.seek 3 @10:54:36.79 Forearm.seek 3 + 300 counts @10:54:37.07 Forearm.reconfigure LooseForearm @10:54:37.12 Hand.close @10:54:37.77 SE.seek :down @10:54:37.99
Forearm.seek :retract @10:54:38.96 Elbow.seek CC @10:54:40.38 SE.coast @10:54:40.62
Elbow.reconfigure LooseElbow @10:54:40.72 Forearm.reconfigure TightForearm @10:54:40.78 Forearm.seek CC @10:54:42.13 CC.seek :closed @10:54:46.39 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.22 @10:54:46.40 Hand.open @10:54:47.21 Forearm.reconfigure LooseForearm @10:54:47.26 Forearm.seek :retract @10:54:48.27 SC.coast @10:54:48.31 CTV.dial CSV,avoiding: :intake @10:54:49.75 CSV.dial CTV @10:54:50.08 CS.seek 5ml @10:55:03.77 delay 1:20 @10:56:23.81 CS.seek 2.5ml @10:56:31.24 delay 40 seconds @10:57:11.28 CS.seek 0ml @10:57:18.68 delay 1:00 @10:58:18.71 PSV.dial :air @10:58:19.20 PS.seek 2.5ml @10:58:45.35 PSV.dial :mixloop @10:58:45.95 delay 3 seconds @10:58:48.97 PSV.dial :air @10:58:49.42 delay 2 seconds @10:58:51.44 PSV.dial :mixloop @10:58:51.91 delay 3 seconds @10:58:54.94 PSV.dial :waste1 @10:58:56.35 PS.seek :empty @10:59:22.53 CBV.dial :waste1 @10:59:23.04 delay 5 seconds @10:59:28.07 CBV.dial AIV @10:59:28.57 delay 10 seconds @10:59:38.60 CBV.dial :waste1 @10:59:39.10 delay 10 seconds @10:59:49.13 CBV.dial AIV @10:59:49.64 delay 10 seconds @10:59:59.67 CTV.dial CSV,avoiding: :intake @10:59:59.68 CBV.dial between AIV and waste1 @11:00:00.73 CSV.dial :air @11:00:01.24 CS.seek 10ml @11:00:27.71 CSV.dial CTV @11:00:28.21 CS.seek 0ml @11:00:54.72 CBV.dial :waste1 @11:00:55.06 delay 10 seconds @11:01:05.09 CTV.dial :air,avoiding: :intake @11:01:06.63 Storing lyfil puck in Collection Clamp to tube 2 @11:01:06.82 SC.reconfigure SCtight @11:01:06.87 SC.seek 2 @11:01:09.09 SC.reconfigure SCloose @11:01:09.31 Elbow.reconfigure TightElbow @11:01:09.36 Elbow.seek 2 @11:01:11.25 Forearm.reconfigure TightForearm @11:01:11.30 Forearm.seek 2 + 700 counts @11:01:12.40 SE.reconfigure SEpick @11:01:12.48 SE.seek beamBreak @11:01:13.81 SE.configure SEconfig @11:01:13.98 Elbow.reconfigure LimpElbow @11:01:14.03 Forearm.reconfigure LooseForearm @11:01:14.07 Forearm.seek 2 @11:01:14.37 Elbow.hold @11:01:14.44 Elbow.reconfigure TightElbow @11:01:14.55 SE.seek 18.09 Pucks below Top Plate @11:01:15.17 Forearm.force -20 @11:01:15.21 Forearm.seek 2 + 338 counts @11:01:15.52 Forearm.reconfigure TightForearm @11:01:15.57 Forearm.seek 2 + 700 counts @11:01:15.93 SE.seek 19.09 Pucks below Top Plate @11:01:16.64 Forearm.reconfigure LooseForearm @11:01:16.69 Forearm.seek :retract @11:01:17.64 Elbow.seek CC @11:01:19.34 Elbow.reconfigure LooseElbow @11:01:19.45 Forearm.seek CC @11:01:20.50 Hand.close @11:01:21.27 CC.seek :open @11:01:25.75 Forearm.seek :retract @11:01:26.78 Elbow.reconfigure TightElbow @11:01:26.83 Elbow.seek 2 @11:01:28.79 Elbow.reconfigure LimpElbow @11:01:28.84 Forearm.seek 2 @11:01:29.86 Elbow.hold @11:01:29.93 Elbow.reconfigure TightElbow @11:01:29.98 Hand.open @11:01:30.77 Forearm.force -20 @11:01:30.82 Forearm.seek 2 + 341 counts @11:01:31.13 Forearm.reconfigure TightForearm @11:01:31.18 Forearm.seek 2 + 700 counts @11:01:32.24 SE.seek :down @11:01:33.14 SE.coast @11:01:33.39
Forearm.reconfigure LooseForearm @11:01:33.44 Forearm.seek :retract @11:01:34.36 SC.coast @11:01:34.40 PSV.dial :air @11:01:35.56 PS.seek 0.499ml @11:01:41.41 delay 3 seconds @11:01:44.43 PSV.dial :mixloop @11:01:44.91 PRV.dial :air @11:01:45.40 delay 3 seconds @11:01:48.43 PRV.dial CBV @11:01:48.94 CBV.dial AIV @11:01:49.48 PRV.dial between CBV and air @11:01:49.83 PRV.dial :air @11:01:50.23 PS.reconfigure PSslow @11:01:50.28 PS.seek 0.599ml @11:01:52.76 PRV.dial :waste2 @11:01:53.55 PS.reconfigure PSconfig @11:01:53.60 PS.seek :empty @11:02:00.51 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:02:00.59 Hand.open @11:02:01.02 Elbow.seek FlushPuck::Garage @11:02:03.05 Elbow.reconfigure LooseElbow @11:02:03.16 Forearm.seek :garage @11:02:04.17 Hand.close @11:02:05.02 Forearm.seek :retract @11:02:06.04 Elbow.reconfigure TightElbow @11:02:06.09 Elbow.seek CC @11:02:08.97 Elbow.reconfigure LooseElbow @11:02:09.08 Forearm.reconfigure TightForearm @11:02:09.13 Forearm.seek CC @11:02:10.52 CC.seek :closed @11:02:14.77 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.09 @11:02:14.85 Hand.open @11:02:15.71 Forearm.reconfigure LooseForearm @11:02:15.76 Forearm.seek :retract @11:02:16.84 Clearing PML for modified lysate @11:02:16.85 CTV.dial :air,avoiding: :intake @11:02:16.86 PRV.dial :waste2 @11:02:16.87 PTV.dial :PRV,avoiding: :puck @11:02:17.68 delay 2 seconds @11:02:19.70 PTV.dial :PRVmixing,avoiding: :puck @11:02:20.51 delay 2 seconds @11:02:22.53 PSV.dial :PML @11:02:22.54 delay 2 seconds @11:02:24.57 PRV.dial :air @11:02:25.36 delay 2 seconds @11:02:27.44 PS.seek 1.499ml @11:02:43.51 delay 5 seconds @11:02:48.55 PS.seek 2.5ml @11:02:59.57 PSV.dial :air @11:03:00.77 PS.seek 0ml @11:03:26.98 mixing lysate with speDil @11:03:26.99 PSV.dial :PML @11:03:27.48 CBV.dial :PRV @11:03:27.49 PRV.dial :air @11:03:27.51 delay 2 seconds @11:03:29.53 PRV.dial :speDil @11:03:30.04 delay 2 seconds @11:03:32.13 PS.reconfigure PSslow1 @11:03:32.18 PS.seek 0.225ml @11:03:55.54 delay 5 seconds @11:04:00.57 PRV.dial CBV @11:04:01.40 delay 2 seconds @11:04:03.48 PS.seek 0.475ml @11:04:29.13 delay 5 seconds @11:04:34.16 PRV.dial :air @11:04:34.66 delay 2 seconds @11:04:36.69 PS.seek 0.965ml @11:05:26.85 delay 10 seconds @11:05:36.88 PRV.dial CBV @11:05:37.37 CTV.dial :air,avoiding: :intake @11:05:37.38 PSV.dial :air @11:05:37.87 PS.reconfigure PSconfig @11:05:37.92 PS.seek 2.5ml @11:05:54.26 delay 3 seconds @11:05:57.28 PSV.dial PTV @11:05:58.07 PTV.dial :PRV,avoiding: :puck @11:05:58.89 PS.seek 0ml @11:06:25.07 PSV.dial :air @11:06:25.86 PS.seek 2.5ml @11:06:52.01 delay 3 seconds @11:06:55.03 PSV.dial PTV @11:06:55.82 PTV.dial :PRV,avoiding: :puck @11:06:55.84 PS.seek 0ml @11:07:21.98 Pushing modified lysate into AIV Injection Loop @11:07:21.99 CBV.dial AIV @11:07:22.00 AIV.dial :bypass @11:07:22.02 PRV.dial CBV @11:07:22.03 PSV.dial :air @11:07:22.83 PS.seek 2.5ml @11:07:49.04 delay 3 seconds @11:07:52.06 PSV.dial :PML @11:07:52.54 PTV.dial :PRVmixing,avoiding: :puck @11:07:53.42 PS.reconfigure PSslow1 @11:07:53.47 PS.seek 1.74ml @11:09:10.79 delay 5 seconds @11:09:15.82 AIV.dial :sample @11:09:16.44 AWV.select :waste @11:09:16.61 PS.reconfigure PSslow2 @11:09:16.66 PS.seek 1.22ml @11:11:03.45 delay 15 seconds @11:11:18.48 AIV.dial :bypass @11:11:19.10 PS.reconfigure PSconfig @11:11:19.15 PS.seek 0ml @11:11:32.39 PSV.dial :air @11:11:32.87 PS.seek 2.5ml @11:11:58.98 PSV.dial :PML @11:11:59.46 PS.seek 0ml @11:12:25.58 AIV.dial :coilToMFB @11:12:25.59 AWV.select :air @11:12:25.76 Extracting Nucleic Acid from AIV @11:12:25.79
Beginning samplePassD @11:12:25.86 SPE.hold 2:46:40,55C @11:12:25.91 ASV.dial :SPEdelivery @11:12:25.99
AWV.select :air @11:12:26.01 AIV.dial :bypass @11:12:26.02 PSV.dial PSR @11:12:26.35 delay 20 seconds @11:12:27.23
Pulling 1.5ml of PSR.killMFB @11:12:27.24 PSR.select :killMFB @11:12:27.37 PS.seek 1.5ml @11:12:43.42 delay 5 seconds @11:12:46.38 primeLysateINT @11:12:46.39 AWV.select :air @11:12:46.40 AIV.dial :coilToMFB @11:12:46.42 ATV.dial AIV @11:12:48.89 AS.reconfigure ASslow1 @11:12:48.99 AS.seek 0.299ml @11:12:49.04
PSV.dial :waste1 @11:12:49.67 PS.seek 0ml @11:13:05.67 PSV.dial PSR @11:13:06.16 PSR.select :air @11:13:06.29 PS.seek 2.5ml @11:13:32.48 PRV.dial CBV @11:13:32.51
CBV.dial PRV @11:13:32.53 PSV.dial :mixloop @11:13:33.69 CTV.dial :air,avoiding: :intake @11:13:33.70 PTV.dial :PRVmixing @11:13:33.73 PS.seek 0ml @11:13:49.66 delay 10 seconds @11:13:59.69 ATV.dial :air2 @11:13:59.86
PSV.dial PSR @11:14:00.56 AS.seek 0.329ml @11:14:01.07
Pulling 1.5ml of PSR.flush @11:14:01.08 PSR.select :flush @11:14:01.21 PS.seek 1.5ml @11:14:06.88 ATV.dial :waste2 @11:14:07.46 AS.reconfigure ASnorm @11:14:07.51 AS.seek :empty @11:14:17.27
delay 5 seconds @11:14:22.30 PSV.dial :waste1 @11:14:22.82 PS.seek 0ml @11:14:24.54 Lysate primed! @11:14:24.55 AFV.select :holdingCoil @11:14:24.56 ATV.dial :air2 @11:14:25.20 AS.reconfigure ASslow1 @11:14:25.25 AS.seek 0.069ml @11:14:38.80
PSV.dial PSR @11:14:39.44 PSR.select :air @11:14:39.57 PS.seek 2.5ml @11:14:39.64 delay 3 seconds @11:14:42.66 ATV.dial :AIV @11:14:43.53 AS.seek 0.469ml @11:15:05.86 PRV.dial CBV @11:15:05.89
CBV.dial PRV @11:15:05.90 PSV.dial :mixloop @11:15:07.06 CTV.dial :air,avoiding: :intake @11:15:07.07 PTV.dial :PRVmixing @11:15:07.10 PS.seek 0ml @11:15:33.21 PSV.dial PSR @11:15:34.42 Pulling 1.5ml of PSR.flush @11:15:34.43 PSR.select :flush @11:15:34.56 PS.seek 1.5ml @11:15:50.52 delay 5 seconds @11:15:55.55 PSV.dial :waste1 @11:15:56.07 PS.seek 0ml @11:16:04.19 delay 5 seconds @11:16:09.28 Solid Phase Extraction Heater at 44.4C @11:16:09.29 ATV.dial ASV @11:16:09.69 ASV.dial :SPEdelivery @11:16:09.77 AS.seek 0.041ml @11:16:12.05
PSV.dial PSR @11:16:12.55 PSR.select :air @11:16:12.68 PS.seek 2.5ml @11:16:38.89 PRV.dial CBV @11:16:38.92
CBV.dial PRV @11:16:38.93 PSV.dial :mixloop @11:16:40.07 CTV.dial :air,avoiding: :intake @11:16:40.08 PTV.dial :PRVmixing @11:16:40.10 PS.seek 0ml @11:17:06.21 PSV.dial PSR @11:17:07.41 Pulling 1.5ml of PSR.flush @11:17:07.42 PSR.select :flush @11:17:07.55 PS.seek 1.5ml @11:17:23.58 delay 5 seconds @11:17:28.61 PSV.dial :waste1 @11:17:29.12 PS.seek 0ml @11:17:36.13 delay 10 seconds @11:17:45.08
PSV.dial PSR @11:17:45.58 PSR.select :air @11:17:45.71 PS.seek 2.5ml @11:17:46.45 rinseLysateLineINT @11:17:46.46 AFV.select :carrierSolution @11:17:46.57 delay 2 seconds @11:17:48.60 AS.reconfigure ASconfig @11:17:48.65 AS.seek 1ml @11:18:12.55 PRV.dial CBV @11:18:12.58
CBV.dial PRV @11:18:12.60 PSV.dial :mixloop @11:18:13.72 CTV.dial :air,avoiding: :intake @11:18:13.73 PTV.dial :PRVmixing @11:18:13.75 PS.reconfigure PSslow @11:18:13.81 PS.seek 1.2ml @11:18:40.61 PSV.dial :waste1 @11:18:42.06 PS.reconfigure PSconfig @11:18:42.10 PS.seek 0ml @11:18:44.09 delay 1 second @11:18:45.11 AFV.select :holdingCoil @11:18:45.21 delay 1 second @11:18:46.24 ATV.dial AIV @11:18:46.81 AIV.dial :coilToMFB @11:18:46.82 AWV.select :waste @11:18:46.94 AS.reconfigure ASslow0 @11:18:46.98 AS.seek 0ml @11:18:55.08
PSV.dial :air @11:18:56.33 PS.seek 2.5ml @11:19:22.49 PSV.dial :mixloop @11:19:22.98 PS.reconfigure PSslow @11:19:23.02 PS.seek 0ml @11:20:14.12 PSV.dial :air @11:20:14.62 PS.reconfigure PSconfig @11:20:14.67 PS.seek 2.5ml @11:20:27.90 ATV.dial :air2 @11:20:28.56 AS.reconfigure ASslow1 @11:20:28.61 AS.seek 0.05ml @11:20:38.95 ATV.dial :AIV @11:20:39.78 AS.seek 0.01ml @11:20:40.87
PSV.dial :mixloop @11:20:41.38 PS.reconfigure PSslow @11:20:41.42 PS.seek 0ml @11:20:48.12 ATV.dial :waste @11:20:49.74 AS.seek :empty @11:20:52.18 ethanol wash @11:20:52.19 ATV.dial :air2 @11:20:53.45 AS.reconfigure ASslow2 @11:20:53.50 AS.seek 0.009ml @11:21:03.87 delay 3 seconds @11:21:06.90 Pulling 0.4ml of Ethanol Wash @11:21:06.91 ATV.dial :ethanol @11:21:07.74 AS.reconfigure ASslow1 @11:21:07.79 AS.seek 0.409ml @11:21:32.56
Cleaning Collection Syringe @11:21:32.57 CSV.dial CSR @11:21:33.11 Pulling 4ml of CSR.flush @11:21:33.12 CSR.select :flush @11:21:33.25 CS.seek 4ml @11:21:44.52 delay 5 seconds @11:21:49.55 CSR.select :air @11:21:49.68 CS.seek 10ml @11:22:06.05 CSV.dial CTV @11:22:06.08
CTV.dial CSV @11:22:07.62 CBV.dial :waste1 @11:22:08.16 CS.seek 0ml @11:22:28.48 delay 3 seconds @11:22:31.51 ATV.dial :air1 @11:22:31.90 delay 3 seconds @11:22:34.71
CSV.dial CSR @11:22:34.98 AS.seek 0.434ml @11:22:35.27
Pulling 4ml of CSR.flush @11:22:35.28 CSR.select :flush @11:22:35.41 CS.seek 4ml @11:22:40.25 delay 3 seconds @11:22:43.28 ATV.dial :ethanol @11:22:43.88 AS.seek 0.419ml @11:22:46.68
delay 5 seconds @11:22:47.17 delay 3 seconds @11:22:50.20 ATV.dial ASV @11:22:51.50 AS.seek 0.092ml @11:22:51.70
CSR.select :air @11:22:51.83 CS.seek 10ml @11:23:08.93 CSV.dial CTV @11:23:08.96
CTV.dial CSV @11:23:09.48 CBV.dial :waste1 @11:23:09.50 CS.seek 0ml @11:23:36.03 Garaging FlushPuck in Collection Clamp @11:23:36.11 Elbow.reconfigure TightElbow @11:23:36.16 Elbow.seek CC @11:23:36.42 Elbow.reconfigure LooseElbow @11:23:36.52 Forearm.seek CC @11:23:37.54 Hand.close @11:23:38.31 CC.seek :open @11:23:42.68 Forearm.seek :retract @11:23:43.69 Elbow.reconfigure TightElbow @11:23:43.74 Elbow.seek :garage @11:23:46.93 Elbow.reconfigure LooseElbow @11:23:47.04 Forearm.reconfigure TightForearm @11:23:47.09 Forearm.seek :garage @11:23:48.24 Hand.open @11:23:49.00 Forearm.reconfigure LooseForearm @11:23:49.05 Forearm.seek :retract @11:23:50.09 Whole Cell Archive sampling 1500ml @11:23:50.11 Loading wcr puck from tube 3 into Collection Clamp @11:23:50.38 SC.reconfigure SCtight @11:23:50.43 SC.seek 3 @11:23:52.62 SC.reconfigure SCloose @11:23:52.86 Elbow.reconfigure TightElbow @11:23:52.91 Elbow.seek 3 @11:23:54.99 Forearm.reconfigure TightForearm @11:23:55.04 Forearm.seek 3 + 700 counts @11:23:56.08 SE.reconfigure SEpick @11:23:56.17 SE.seek beamBreak @11:23:57.45 delay 3 seconds @11:23:59.24 SE.configure SEconfig @11:23:59.41 Elbow.seek 3 @11:23:59.62 Forearm.seek 3 + 300 counts @11:23:59.89 Forearm.reconfigure LooseForearm @11:23:59.94 Hand.close @11:24:00.47 ATV.dial :waste2 @11:24:00.70 SE.seek :down @11:24:00.94 Forearm.seek :retract @11:24:01.22 AS.reconfigure ASconfig @11:24:01.27 AS.seek :empty @11:24:01.90 Elbow.seek CC @11:24:03.47 SE.coast @11:24:03.60 Elbow.reconfigure LooseElbow @11:24:03.71 Forearm.reconfigure TightForearm @11:24:03.76 Forearm.seek CC @11:24:05.85 CC.seek :closed @11:24:06.91 ATV.dial :air2 @11:24:07.36 AS.reconfigure ASslow1 @11:24:07.44 AS.seek 1ml @11:24:10.11 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.24 @11:24:10.13 Hand.open @11:24:11.00 Forearm.reconfigure LooseForearm @11:24:11.05 Forearm.seek :retract @11:24:12.09 SC.coast @11:24:12.13 collectSample 1500ml w/bubble point of 55psi @11:24:12.15 Priming 7ml @11:24:12.16 Intake.select :close @11:24:12.17 Exhaust.select :close @11:24:12.19 SS.hold @11:24:12.42 Calibrating pressure sensors @11:24:12.43 CTV.dial :air @11:24:13.90 SSV.dial :spit,avoiding: :exhaust @11:24:13.92 SS.reconfigure SSfast @11:24:13.97 SS.seek 5ml @11:24:16.83 SSV.dial CBV @11:24:16.86 CBV.dial SSV @11:24:18.43 delay 3.8 seconds @11:24:22.38 SSV.dial :spit @11:24:23.83 SS.seek 0ml @11:24:26.54 SSV.dial CBV @11:24:28.01 CTV.dial :intake @11:24:28.85 SSV.dial CBV @11:24:28.87 CBV.dial SSV @11:24:28.90 Allowing 40s for 7.0ml of remaining 7.0ml @11:24:28.91 Intake.select :open @11:24:31.09 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:24:31.11 SS.reconfigure SSinhale.with maxSpeed: 150 @11:24:31.15 SS.seek 7ml @11:24:36.94 Stroke completed -- waiting for pressure to equalize ... @11:24:50.23 Intake.select :close @11:24:50.39 SSV.dial :exhaust @11:24:51.24 Exhaust.select :open @11:24:52.10 SS.reconfigure SSexhale @11:24:52.15 SS.seek 0ml @11:24:55.88 Exhaust.select :close @11:24:55.99 Sampling 1500.0ml @11:24:56.01 Intake.select :close @11:24:56.02 Exhaust.select :close @11:24:56.03 SS.reconfigure SSconfig @11:24:56.08 SS.hold @11:24:56.30 CTV.dial :intake @11:24:56.32 SSV.dial CBV @11:24:56.36 CBV.dial SSV @11:24:57.23 Allowing 130s for 25.0ml of remaining 1500.0ml @11:24:57.25 Intake.select :open @11:24:59.43 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:24:59.45 SS.reconfigure SSinhale.with maxSpeed: 150 @11:24:59.49 SS.seek 25ml @11:25:03.51 Vacuum too high -- waiting up to 40s @11:25:05.90 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:25:05.92 SS.reconfigure SSinhale.with maxSpeed: 135 @11:25:05.97 SS.seek 25ml @11:25:09.10 Vacuum too high -- waiting up to 40s @11:25:11.73 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:25:11.75 SS.reconfigure SSinhale.with maxSpeed: 122 @11:25:11.80 SS.seek 25ml @11:25:15.01 Vacuum too high -- waiting up to 40s @11:25:17.63 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:25:17.66 SS.reconfigure SSinhale.with maxSpeed: 110 @11:25:17.70 SS.seek 25ml @11:25:20.75 Vacuum too high -- waiting up to 40s @11:25:23.85 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:25:23.87 SS.reconfigure SSinhale.with maxSpeed: 99 @11:25:23.92 SS.seek 25ml @11:25:27.28 Vacuum too high -- waiting up to 40s @11:25:29.57 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:25:29.59 SS.reconfigure SSinhale.with maxSpeed: 89 @11:25:29.64 SS.seek 25ml @11:25:32.57 Vacuum too high -- waiting up to 40s @11:25:34.94 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:25:34.96 SS.reconfigure SSinhale.with maxSpeed: 80 @11:25:35.02 SS.seek 25ml @11:25:37.98 Vacuum too high -- waiting up to 40s @11:25:40.33 SS.reconfigure SSinhale.with maxSpeed: 72 @11:25:40.38 SS.seek 25ml @11:25:43.17 Stroke completed -- waiting for pressure to equalize ... @11:25:48.27 Intake.select :close @11:25:48.43 SSV.dial :exhaust @11:25:49.27 Exhaust.select :open @11:25:49.39 SS.reconfigure SSexhale @11:25:49.44 SS.seek 0ml @11:25:58.33 Exhaust.select :close @11:25:58.45 SSV.dial CBV @11:25:58.47 CBV.dial SSV @11:25:59.33 Allowing 130s for 25.0ml of remaining 1475.0ml @11:25:59.34 Intake.select :open @11:26:01.53 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:26:01.55 SS.reconfigure SSinhale.with maxSpeed: 72 @11:26:01.59 SS.seek 25ml @11:26:07.00 Vacuum too high -- waiting up to 40s @11:26:08.87 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:26:08.88 SS.reconfigure SSinhale.with maxSpeed: 65 @11:26:08.93 SS.seek 25ml @11:26:13.00 Vacuum too high -- waiting up to 40s @11:26:15.15 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:26:15.17 SS.reconfigure SSinhale.with maxSpeed: 59 @11:26:15.22 SS.seek 25ml @11:26:20.73 Vacuum too high -- waiting up to 40s @11:26:22.60 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:26:22.63 SS.reconfigure SSinhale.with maxSpeed: 53 @11:26:22.67 SS.seek 25ml @11:26:29.50 Vacuum too high -- waiting up to 40s @11:26:31.38 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:26:31.40 SS.reconfigure SSinhale.with maxSpeed: 48 @11:26:31.46 SS.seek 25ml @11:26:40.53 Vacuum too high -- waiting up to 40s @11:26:42.46 SS.reconfigure SSinhale.with maxSpeed: 43 @11:26:42.51 SS.seek 25ml @11:26:47.46 Stroke completed -- waiting for pressure to equalize ... @11:26:51.97 Intake.select :close @11:26:52.13 SSV.dial :exhaust @11:26:53.00 Exhaust.select :open @11:26:53.12 SS.reconfigure SSexhale @11:26:53.17 SS.seek 0ml @11:27:02.01 Exhaust.select :close @11:27:02.13 SSV.dial CBV @11:27:02.15 CBV.dial SSV @11:27:03.01 Allowing 130s for 25.0ml of remaining 1450.0ml @11:27:03.02 Intake.select :open @11:27:05.93 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:27:05.95 SS.reconfigure SSinhale.with maxSpeed: 43 @11:27:05.99 SS.seek 25ml @11:27:25.23 Vacuum too high -- waiting up to 40s @11:27:27.12 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:27:27.14 SS.reconfigure SSinhale.with maxSpeed: 39 @11:27:27.19 SS.seek 25ml @11:27:28.98 delay 5 seconds @11:27:34.01 ATV.dial ASV @11:27:34.98 ASV.dial :SPEdelivery @11:27:35.00 AS.seek 0.02ml @11:27:52.46 Stroke completed -- waiting for pressure to equalize ... @11:27:58.25 Intake.select :close @11:27:58.41 SSV.dial :exhaust @11:27:59.26 Exhaust.select :open @11:27:59.38 SS.reconfigure SSexhale @11:27:59.43 SS.seek 0ml @11:28:08.21 Exhaust.select :close @11:28:08.33 SSV.dial CBV @11:28:08.35 CBV.dial SSV @11:28:09.23 Allowing 130s for 25.0ml of remaining 1425.0ml @11:28:09.24 Intake.select :open @11:28:11.43 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:28:11.45 SS.reconfigure SSinhale.with maxSpeed: 39 @11:28:11.49 SS.seek 25ml @11:28:41.51 Vacuum too high -- waiting up to 40s @11:28:43.39 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:28:43.41 SS.reconfigure SSinhale.with maxSpeed: 35 @11:28:43.46 SS.seek 25ml @11:29:01.37 Stroke completed -- waiting for pressure to equalize ... @11:29:06.54 Intake.select :close @11:29:06.70 SSV.dial :exhaust @11:29:07.55 Exhaust.select :open @11:29:07.67 SS.reconfigure SSexhale @11:29:07.72 SS.seek 0ml @11:29:16.68 Exhaust.select :close @11:29:16.80 SSV.dial CBV @11:29:16.82 CBV.dial SSV @11:29:18.41 Allowing 130s for 25.0ml of remaining 1400.0ml @11:29:18.42 Intake.select :open @11:29:20.61 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:29:20.63 SS.reconfigure SSinhale.with maxSpeed: 35 @11:29:20.67 SS.seek 25ml @11:30:11.37 Stroke completed -- waiting for pressure to equalize ... @11:30:17.16 Intake.select :close @11:30:17.32 SSV.dial :exhaust @11:30:18.18 Exhaust.select :open @11:30:18.30 SS.reconfigure SSexhale @11:30:18.35 SS.seek 0ml @11:30:27.49 Exhaust.select :close @11:30:27.61 SSV.dial CBV @11:30:27.63 CBV.dial SSV @11:30:28.51 Allowing 130s for 25.0ml of remaining 1375.0ml @11:30:28.52 Intake.select :open @11:30:30.71 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:30:30.73 SS.reconfigure SSinhale.with maxSpeed: 35 @11:30:30.78 SS.seek 25ml @11:30:52.36 delay 5 seconds @11:30:57.39 ATV.dial :air2 @11:30:58.45 AS.seek 0.52ml @11:31:18.64 Vacuum too high -- waiting up to 40s @11:31:20.52 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:31:20.54 SS.reconfigure SSinhale.with maxSpeed: 32 @11:31:20.59 SS.seek 25ml @11:31:23.69 Stroke completed -- waiting for pressure to equalize ... @11:31:28.17 Intake.select :close @11:31:28.33 SSV.dial :exhaust @11:31:29.19 Exhaust.select :open @11:31:29.31 SS.reconfigure SSexhale @11:31:29.36 SS.seek 0ml @11:31:38.29 Exhaust.select :close @11:31:38.41 SSV.dial CBV @11:31:38.43 CBV.dial SSV @11:31:39.30 Allowing 130s for 25.0ml of remaining 1350.0ml @11:31:39.31 Intake.select :open @11:31:41.50 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:31:41.52 SS.reconfigure SSinhale.with maxSpeed: 32 @11:31:41.56 SS.seek 25ml @11:32:36.84 Stroke completed -- waiting for pressure to equalize ... @11:32:39.33 delay 5 seconds @11:32:43.35 Intake.select :close @11:32:43.52 SSV.dial :exhaust @11:32:44.38 Exhaust.select :open @11:32:44.43 ATV.dial ASV @11:32:44.50 SS.reconfigure SSexhale @11:32:44.55 SS.seek 0ml @11:32:45.39 ASV.dial :wasteR @11:32:45.76 AS.seek 0.3ml @11:32:53.84 Exhaust.select :close @11:32:53.96 SSV.dial CBV @11:32:53.98 CBV.dial SSV @11:32:54.84 Allowing 130s for 25.0ml of remaining 1325.0ml @11:32:54.85 Intake.select :open @11:32:57.03 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:32:57.05 SS.reconfigure SSinhale.with maxSpeed: 32 @11:32:57.10 SS.seek 25ml @11:33:30.28 delay 20 seconds @11:33:44.98 Vacuum too high -- waiting up to 40s @11:33:47.13 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:33:47.14 SS.reconfigure SSinhale.with maxSpeed: 29 @11:33:47.19 SS.seek 25ml @11:33:50.31 AS.seek 0.07ml @11:33:54.94 Stroke completed -- waiting for pressure to equalize ... @11:34:00.05 Intake.select :close @11:34:00.21 SSV.dial :exhaust @11:34:01.08 Exhaust.select :open @11:34:01.20 SS.reconfigure SSexhale @11:34:01.25 SS.seek 0ml @11:34:10.37 Exhaust.select :close @11:34:10.49 SSV.dial CBV @11:34:10.51 CBV.dial SSV @11:34:11.38 Allowing 130s for 25.0ml of remaining 1300.0ml @11:34:11.40 Intake.select :open @11:34:13.58 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:34:13.60 SS.reconfigure SSinhale.with maxSpeed: 29 @11:34:13.64 SS.seek 25ml @11:34:36.80 delay 20 seconds @11:34:56.83 ATV.dial :waste2 @11:34:57.58 AS.reconfigure ASconfig @11:34:57.63 AS.seek :empty @11:35:02.00 Elution @11:35:02.01 ATV.dial :air1 @11:35:03.14 AS.reconfigure ASslow1 @11:35:03.19 AS.seek 0.049ml @11:35:13.55 delay 3 seconds @11:35:14.19 Stroke completed -- waiting for pressure to equalize ... @11:35:16.57 ATV.dial :ElutionWater @11:35:17.28 AS.seek 0.109ml @11:35:21.42 Intake.select :close @11:35:21.58 SSV.dial :exhaust @11:35:22.43 Exhaust.select :open @11:35:22.55 SS.reconfigure SSexhale @11:35:22.60 SS.seek 0ml @11:35:29.58 delay 3 seconds @11:35:32.09 Exhaust.select :close @11:35:32.21 SSV.dial CBV @11:35:32.23 CBV.dial SSV @11:35:32.60 ATV.dial ASV,via: :air2 @11:35:33.10 Allowing 130s for 25.0ml of remaining 1275.0ml @11:35:33.11 Intake.select :open @11:35:34.69 ASV.dial :SPEelution @11:35:35.31 AFV.select :carrierSolution @11:35:35.33 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:35:35.38 SS.reconfigure SSinhale.with maxSpeed: 29 @11:35:35.46 SS.seek 25ml @11:35:35.58 AS.reconfigure ASnorm @11:35:35.63 AS.seek 0.759ml @11:36:08.68 delay 3 seconds @11:36:11.70 AFV.select :holdingCoil @11:36:11.85 AS.reconfigure ASslow1 @11:36:11.91 AS.seek 0.474ml @11:36:17.97 Vacuum too high -- waiting up to 40s @11:36:20.38 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:36:20.40 SS.reconfigure SSinhale.with maxSpeed: 26 @11:36:20.45 SS.seek 25ml @11:36:40.82 Stroke completed -- waiting for pressure to equalize ... @11:36:48.44 Intake.select :close @11:36:48.60 SSV.dial :exhaust @11:36:49.46 Exhaust.select :open @11:36:49.58 SS.reconfigure SSexhale @11:36:49.63 SS.seek 0ml @11:36:59.29 Exhaust.select :close @11:36:59.40 SSV.dial CBV @11:36:59.43 CBV.dial SSV @11:37:00.29 Allowing 130s for 25.0ml of remaining 1250.0ml @11:37:00.30 Intake.select :open @11:37:02.48 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:37:02.50 SS.reconfigure SSinhale.with maxSpeed: 26 @11:37:02.55 SS.seek 25ml @11:37:09.50 delay 5 seconds @11:37:14.60 AS.reconfigure ASslow2 @11:37:14.65 AS.seek 0.439ml @11:37:50.83 delay 1:00 @11:37:58.76 Vacuum too high -- waiting up to 40s @11:38:01.41 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:38:01.43 SS.reconfigure SSinhale.with maxSpeed: 23 @11:38:01.48 SS.seek 25ml @11:38:14.26 Stroke completed -- waiting for pressure to equalize ... @11:38:21.30 Intake.select :close @11:38:21.46 SSV.dial :exhaust @11:38:22.32 Exhaust.select :open @11:38:22.44 SS.reconfigure SSexhale @11:38:22.49 SS.seek 0ml @11:38:31.48 Exhaust.select :close @11:38:31.60 SSV.dial CBV @11:38:31.62 CBV.dial SSV @11:38:32.48 Allowing 130s for 25.0ml of remaining 1225.0ml @11:38:32.49 Intake.select :open @11:38:34.68 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:38:34.70 SS.reconfigure SSinhale.with maxSpeed: 23 @11:38:34.74 SS.seek 25ml @11:38:50.86 pushing eluate to holding coil @11:38:50.93 AS.seek 0.279ml @11:39:51.24 Stroke completed -- waiting for pressure to equalize ... @11:40:02.02 Intake.select :close @11:40:02.18 SSV.dial :exhaust @11:40:03.04 Exhaust.select :open @11:40:03.16 SS.reconfigure SSexhale @11:40:03.21 SS.seek 0ml @11:40:13.12 Exhaust.select :close @11:40:13.24 SSV.dial CBV @11:40:13.26 CBV.dial SSV @11:40:14.13 Allowing 130s for 25.0ml of remaining 1200.0ml @11:40:14.14 Intake.select :open @11:40:16.36 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:40:16.38 SS.reconfigure SSinhale.with maxSpeed: 23 @11:40:16.43 SS.seek 25ml @11:40:55.70 Vacuum too high -- waiting up to 40s @11:40:58.89 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:40:58.91 SS.reconfigure SSinhale.with maxSpeed: 21 @11:40:58.95 SS.seek 25ml @11:41:31.98 delay 30 seconds @11:41:39.89 Stroke completed -- waiting for pressure to equalize ... @11:41:51.99 Intake.select :close @11:41:52.15 SSV.dial :exhaust @11:41:53.01 Exhaust.select :open @11:41:53.13 SS.reconfigure SSexhale @11:41:53.18 SS.seek 0ml @11:42:02.01 ATV.dial :waste,via: :air3 @11:42:02.37 Exhaust.select :close @11:42:02.49 SSV.dial CBV @11:42:02.51 CBV.dial SSV @11:42:03.39 Allowing 130s for 25.0ml of remaining 1175.0ml @11:42:03.40 Intake.select :open @11:42:03.70 AS.reconfigure ASconfig @11:42:03.75 AS.seek :empty @11:42:05.59 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:42:05.61 SS.reconfigure SSinhale.with maxSpeed: 21 @11:42:05.65 SS.seek 25ml @11:42:20.13 Parking Bleach in LysateSource line and SPEcolumn @11:42:20.14 ATV.dial :air3 @11:42:20.98 AS.reconfigure ASslow1 @11:42:21.03 AS.seek 0.029ml @11:42:27.38 ATV.dial :ElutionWater @11:42:28.00 AS.seek 0.059ml @11:42:34.30 ATV.dial :air3 @11:42:35.66 AS.seek 0.069ml @11:42:37.93 delay 2 seconds @11:42:39.95 ATV.dial :bleach @11:42:40.44 AS.seek 0.769ml @11:42:44.64 Vacuum too high -- waiting up to 40s @11:42:48.07 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:42:48.09 SS.reconfigure SSinhale.with maxSpeed: 19 @11:42:48.15 SS.seek 25ml @11:43:33.39 Vacuum too high -- waiting up to 40s @11:43:37.82 SS.reconfigure SSinhale.with maxSpeed: 17 @11:43:37.87 SS.seek 25ml @11:43:43.45 Stroke completed -- waiting for pressure to equalize ... @11:43:53.60 Intake.select :close @11:43:53.76 SSV.dial :exhaust @11:43:55.34 Exhaust.select :open @11:43:55.46 SS.reconfigure SSexhale @11:43:55.51 SS.seek 0ml @11:44:04.79 Exhaust.select :close @11:44:04.90 SSV.dial CBV @11:44:04.94 CBV.dial SSV @11:44:05.79 Allowing 130s for 25.0ml of remaining 1150.0ml @11:44:05.80 Intake.select :open @11:44:07.98 Intake @31.7psia, vacuum from 20.0 to 24.0psi @11:44:08.00 SS.reconfigure SSinhale.with maxSpeed: 17 @11:44:08.06 SS.seek 25ml @11:45:01.48 delay 3 seconds @11:45:04.51 ATV.dial :air3 @11:45:05.06 delay 3 seconds @11:45:08.15 AS.seek 0.794ml @11:45:13.45 delay 3 seconds @11:45:16.48 ATV.dial :bleach @11:45:17.10 AS.seek 0.779ml @11:45:20.37 delay 3 seconds @11:45:21.95 Vacuum too high -- waiting up to 40s @11:45:23.39 ATV.dial :AIV @11:45:24.67 AS.reconfigure ASconfig @11:45:24.72 AS.seek 0.015ml @11:45:26.28 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:45:26.30 SS.reconfigure SSinhale.with maxSpeed: 15 @11:45:26.34 SS.seek 25ml @11:45:59.93 Stroke completed -- waiting for pressure to equalize ... @11:46:08.91 ATV.dial :waste @11:46:10.42 AS.seek :empty @11:46:11.59 ATV.dial :air3 @11:46:12.28 AS.reconfigure ASslow1 @11:46:12.33 AS.seek 0.009ml @11:46:13.93 Intake.select :close @11:46:14.10 SSV.dial :exhaust @11:46:14.96 Exhaust.select :open @11:46:15.08 SS.reconfigure SSexhale @11:46:15.14 SS.seek 0ml @11:46:15.49 delay 2 seconds @11:46:17.51 ATV.dial :bleach @11:46:17.97 AS.seek 0.579ml @11:46:24.23 Exhaust.select :close @11:46:24.35 SSV.dial CBV @11:46:24.37 CBV.dial SSV @11:46:25.25 Allowing 130s for 25.0ml of remaining 1125.0ml @11:46:25.26 Intake.select :open @11:46:27.44 Intake @31.8psia, vacuum from 20.0 to 24.0psi @11:46:27.46 SS.reconfigure SSinhale.with maxSpeed: 15 @11:46:27.51 SS.seek 25ml @11:47:42.95 Vacuum too high -- waiting up to 40s @11:47:47.90 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:47:47.92 SS.reconfigure SSinhale.with maxSpeed: 14 @11:47:47.98 SS.seek 25ml @11:48:12.89 delay 3 seconds @11:48:15.92 ATV.dial :air3 @11:48:16.50 delay 3 seconds @11:48:19.59 AS.seek 0.604ml @11:48:24.88 delay 3 seconds @11:48:27.91 ATV.dial :bleach @11:48:28.53 AS.seek 0.589ml @11:48:31.82 delay 3 seconds @11:48:33.45 Stroke completed -- waiting for pressure to equalize ... @11:48:34.84 ATV.dial :ASV @11:48:35.34 SS.reconfigure SSconfig @11:48:35.40 SS.hold @11:48:35.53 Intake.select :close @11:48:35.69 ShallowSampler::Clogged in WCR -- progress too slow after 400.0ml @11:48:35.71 SSV.dial :exhaust @11:48:35.89 ASV.dial :wasteR @11:48:36.49 AS.seek 0ml @11:48:36.56 Exhaust.select :open @11:48:36.68 SS.reconfigure SSexhale @11:48:36.73 SS.seek 0ml @11:48:46.31 Exhaust.select :close @11:48:46.42 Sampled 400.0ml @11:48:46.44 Drying puck before fixing for 4:00 @11:48:46.45 CBV.dial :waste2b @11:48:47.30 delay 1:00 @11:49:47.33 CSV.dial :air @11:49:48.52 CS.reconfigure CSfast @11:49:48.61 CS.seek 10ml @11:50:14.34 Pumping wcr puck from tube 3 up to 55psi... @11:50:14.36 CBV.dial SSV @11:50:15.78 CBV.dial between CSV and waste2b @11:50:16.86 CSV.dial between air and CTV @11:50:17.24 CS.seek 7.11ml @11:50:25.25 CTV.dial CSV,avoiding: :air @11:50:25.27 CSV.dial CTV @11:50:26.17 CS.reconfigure CSpressurize @11:50:26.22 CS.seek 0ml @11:50:35.23 delay 1:00 @11:50:42.95 CBV.dial :waste2b @11:50:43.32 CS.reconfigure CSconfig @11:50:43.37 CS.seek :empty @11:50:46.30 delay 1:00 @11:51:35.26 AFV.select :carrierSolution @11:51:35.36 delay 2 seconds @11:51:37.39 AS.reconfigure ASconfig @11:51:37.44 AS.seek 1ml @11:51:46.33 CTV.dial :air,avoiding: :intake @11:51:47.83 Fix #1 of 2 (with rnal) for 2:00 @11:51:47.84 CSV.dial CSR @11:51:48.38 Pulling 2ml of CSR.rnal @11:51:48.40 CSR.select :rnal @11:51:48.52 CS.seek 2ml @11:51:54.74 delay 5 seconds @11:51:59.77 CSR.select :air @11:51:59.90 CS.reconfigure CSfast @11:51:59.95 CS.seek 10ml @11:52:20.46 Pumping wcr puck from tube 3 up to 55psi... @11:52:20.48 CBV.dial SSV @11:52:21.88 CBV.dial between CSV and waste2b @11:52:22.95 CSV.dial between air and CTV @11:52:23.61 CS.seek 7.2ml @11:52:31.47 CTV.dial CSV,avoiding: :air @11:52:31.49 CSV.dial CTV @11:52:33.04 CS.reconfigure CSpressurize @11:52:33.09 CS.seek 0ml @11:52:35.20 delay 1 second @11:52:36.22 AFV.select :holdingCoil @11:52:36.32 delay 1 second @11:52:37.35 ATV.dial ASV @11:52:37.37 ASV.dial :wasteR @11:52:37.39 AS.reconfigure ASslow1 @11:52:37.44 AS.seek 0ml @11:52:51.58 delay 2:00 @11:54:51.62 CBV.dial :waste2b @11:54:51.98 CS.reconfigure CSconfig @11:54:52.03 CS.seek :empty @11:54:53.34 delay 1:00 @11:55:53.37 CTV.dial :air,avoiding: :intake @11:55:54.84 Fix #2 of 2 (with rnal) for 2:00 @11:55:54.86 CSV.dial CSR @11:55:55.40 Pulling 2ml of CSR.rnal @11:55:55.42 CSR.select :rnal @11:55:55.54 CS.seek 2ml @11:55:58.80 delay 20 seconds @11:56:01.75 delay 5 seconds @11:56:06.78 CSR.select :air @11:56:06.90 CS.reconfigure CSfast @11:56:06.95 CS.seek 10ml @11:56:18.83 ATV.dial :air3 @11:56:20.32 AS.seek 0.59ml @11:56:27.53 Pumping wcr puck from tube 3 up to 55psi... @11:56:27.54 CBV.dial SSV @11:56:28.94 CBV.dial between CSV and waste2b @11:56:30.02 CSV.dial between air and CTV @11:56:30.82 CS.seek 7.123ml @11:56:38.79 CTV.dial CSV,avoiding: :air @11:56:38.81 CSV.dial CTV @11:56:40.38 CS.reconfigure CSpressurize @11:56:40.43 CS.seek 0ml @11:56:57.80 delay 2:00 @11:58:19.24 delay 3 seconds @11:58:22.27 ATV.dial :ASV @11:58:23.66 ASV.dial :wasteR @11:58:23.75 AS.seek 0.09ml @11:58:57.83 CBV.dial :waste2b @11:58:58.20 CS.reconfigure CSconfig @11:58:58.26 CS.seek :empty @11:59:00.63 delay 1:00 @12:00:00.66 CTV.dial :air,avoiding: :intake @12:00:02.21 Storing wcr puck in Collection Clamp to tube 2 @12:00:02.40 SC.reconfigure SCtight @12:00:02.46 SC.seek 2 @12:00:04.58 delay 30 seconds @12:00:04.68 SC.reconfigure SCloose @12:00:04.91 Elbow.reconfigure TightElbow @12:00:04.96 Elbow.seek 2 @12:00:06.85 Forearm.reconfigure TightForearm @12:00:06.90 Forearm.seek 2 + 700 counts @12:00:07.95 SE.reconfigure SEpick @12:00:08.03 SE.seek beamBreak @12:00:09.37 SE.configure SEconfig @12:00:09.55 Elbow.reconfigure LimpElbow @12:00:09.60 Forearm.reconfigure LooseForearm @12:00:09.65 Forearm.seek 2 @12:00:09.93 Elbow.hold @12:00:10.01 Elbow.reconfigure TightElbow @12:00:10.12 SE.seek 19.09 Pucks below Top Plate @12:00:10.70 Forearm.force -20 @12:00:10.74 Forearm.seek 2 + 343 counts @12:00:11.08 Forearm.reconfigure TightForearm @12:00:11.14 Forearm.seek 2 + 700 counts @12:00:11.42 SE.seek 20.09 Pucks below Top Plate @12:00:12.25 Forearm.reconfigure LooseForearm @12:00:12.30 Forearm.seek :retract @12:00:13.21 Elbow.seek CC @12:00:14.92 Elbow.reconfigure LooseElbow @12:00:15.71 Forearm.seek CC @12:00:16.74 Hand.close @12:00:17.50 CC.seek :open @12:00:21.99 Forearm.seek :retract @12:00:23.05 Elbow.reconfigure TightElbow @12:00:23.10 Elbow.seek 2 @12:00:25.10 Elbow.reconfigure LimpElbow @12:00:25.16 Forearm.seek 2 @12:00:26.18 Elbow.hold @12:00:26.26 Elbow.reconfigure TightElbow @12:00:26.31 Hand.open @12:00:27.26 Forearm.force -20 @12:00:27.30 Forearm.seek 2 + 342 counts @12:00:27.63 Forearm.reconfigure TightForearm @12:00:27.68 Forearm.seek 2 + 700 counts @12:00:28.08 SE.seek :down @12:00:28.89 SE.coast @12:00:29.23 Forearm.reconfigure LooseForearm @12:00:29.28 Forearm.seek :retract @12:00:30.20 SC.coast @12:00:30.25 Starting finalPurge @12:00:30.26 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:00:30.34 Hand.open @12:00:30.76 Elbow.seek FlushPuck::Garage @12:00:32.87 Elbow.reconfigure LooseElbow @12:00:32.97 Forearm.seek :garage @12:00:33.95 Hand.close @12:00:34.66 ATV.dial :extSrc @12:00:34.80
Forearm.seek :retract @12:00:35.53 delay 3 seconds @12:00:35.79
Elbow.reconfigure TightElbow @12:00:35.84 Elbow.seek CC @12:00:38.56 ATV.dial :ElutionWater @12:00:38.72
Elbow.reconfigure LooseElbow @12:00:38.82 Forearm.reconfigure TightForearm @12:00:38.87 Forearm.seek CC @12:00:39.67 AS.seek 0.12ml @12:00:40.20
CC.seek :closed @12:00:45.24 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.18 @12:00:45.31 Hand.open @12:00:46.03 ATV.dial :air3 @12:00:46.19
Forearm.reconfigure LooseForearm @12:00:46.24 Forearm.seek :retract @12:00:46.65 AS.seek 0.13ml @12:00:47.27
Flushing the Collection Valve::Manifold @12:00:47.41 CSV.dial CSR @12:00:47.96 Pulling 5ml of CSR.flush @12:00:47.97 CSR.select :flush @12:00:48.09 CS.seek 5ml @12:00:48.94 delay 2 seconds @12:00:50.97 ATV.dial :bleach @12:00:51.43 AS.seek 0.23ml @12:01:01.94
delay 5 seconds @12:01:06.97 CSR.select :air @12:01:07.15 CS.reconfigure CSfast @12:01:07.20 CS.seek 7.5ml @12:01:11.86 delay 3 seconds @12:01:14.38
CSV.dial :waste @12:01:14.88 ATV.dial :air3 @12:01:15.42 delay 3 seconds @12:01:15.82
CS.reconfigure CSconfig @12:01:15.87 CS.seek 0ml @12:01:18.51 AS.seek 0.255ml @12:01:23.83 delay 3 seconds @12:01:26.86 ATV.dial :bleach @12:01:27.46 AS.seek 0.24ml @12:01:30.77 delay 3 seconds @12:01:33.80 ATV.dial :ASV @12:01:34.83 ASV.dial :SPEdelivery @12:01:35.21 AFV.select :carrierSolution @12:01:35.32 delay 2 seconds @12:01:36.08
Flushing lower manifold's waste1 port @12:01:36.22 CSV.dial :flush @12:01:36.72 CS.reconfigure CSfast @12:01:36.77 CS.seek 5ml @12:01:37.34 AS.reconfigure ASconfig @12:01:37.39 AS.seek 0.5ml @12:01:49.91
delay 5 seconds @12:01:52.64 delay 1 second @12:01:53.66 AFV.select :holdingCoil @12:01:53.81 AS.reconfigure ASslow1 @12:01:53.86 AS.seek 0.17ml @12:01:54.93
CSV.dial :air @12:01:55.49 CS.seek 7ml @12:02:01.41 CSV.dial CTV @12:02:01.44
CTV.dial CSV @12:02:02.99 CBV.dial :waste1 @12:02:03.84 CS.seek 0ml @12:02:21.69 Flushing lower manifold's waste2b port @12:02:21.83 CSV.dial :flush @12:02:22.67 CS.seek 5ml @12:02:35.69 delay 5 seconds @12:02:40.72 CSV.dial :air @12:02:41.31 CS.seek 7ml @12:02:47.16 CSV.dial CTV @12:02:47.21
CTV.dial CSV @12:02:47.66 CBV.dial :waste2b @12:02:48.52 CS.seek 0ml @12:03:00.53 SPE.hold 2:46:40,95C @12:03:00.57 delay 3:00 @12:03:06.42
Flushing upper manifold @12:03:06.55 CSV.dial :flush @12:03:07.41 CS.seek 5ml @12:03:21.01 delay 5 seconds @12:03:26.04 CSV.dial :air @12:03:26.62 CS.seek 7.001ml @12:03:32.66 CSV.dial CBV @12:03:32.71
CBV.dial CSV @12:03:34.02 CTV.dial :air @12:03:35.55 CS.seek 0ml @12:03:54.20 delay 7 seconds @12:04:01.24 CS.seek 3ml @12:04:09.88 CSV.dial :waste @12:04:10.39 CS.seek 0ml @12:04:19.01 CSV.dial :flush @12:04:19.53 CS.seek 3ml @12:04:28.16 delay 5 seconds @12:04:33.19 CSV.dial :air @12:04:33.78 CS.seek 5ml @12:04:39.76 CSV.dial :air @12:04:39.78 CS.seek 0ml @12:04:53.70 delay 7 seconds @12:05:00.74 CS.seek 3ml @12:05:09.23 CSV.dial :waste @12:05:10.08 CS.seek 0ml @12:05:18.85 Disinfecting external Intake with mfbkill @12:05:18.99 CSV.dial CSR @12:05:20.56 Pulling 7.999ml of CSR.mfbkill CSR.select :mfbkill @12:05:20.69 CS.seek 8ml @12:05:41.70 delay 5 seconds @12:05:46.73 CSR.select :air @12:05:46.86 CS.seek 10ml @12:05:52.95 CSV.dial CBV @12:05:52.98
CBV.dial CSV @12:05:54.14 CTV.dial :intake @12:05:54.99 Intake.select :open @12:05:55.11 CS.seek 2ml @12:06:00.60 AFV.select :carrierSolution @12:06:00.70 delay 2 seconds @12:06:02.73 AS.reconfigure ASconfig @12:06:02.78 AS.seek 1ml @12:06:17.39
Intake.select :close @12:06:17.50 CTV.dial :air @12:06:18.37 CS.reconfigure CSconfig @12:06:18.42 CS.seek 0ml @12:06:24.57 Flushing lower manifold's waste1 port @12:06:24.58 CSV.dial CSR @12:06:25.82 Pulling 4ml of CSR.mfbkill @12:06:25.83 CSR.select :mfbkill @12:06:25.96 CS.seek 4ml @12:06:37.10 delay 5 seconds @12:06:42.13 CSR.select :air @12:06:42.26 CS.reconfigure CSfast @12:06:42.30 CS.seek 10ml @12:06:50.76 delay 1 second @12:06:51.78 AFV.select :holdingCoil @12:06:51.88 delay 1 second @12:06:52.91 ASV.dial :wasteD @12:06:53.98 AS.seek 0ml @12:06:58.30 CSV.dial CTV @12:06:58.32
CTV.dial CSV @12:06:59.96 CBV.dial :waste1 @12:07:00.49 CS.seek 0ml @12:07:26.54 Flushing lower manifold's waste2b port @12:07:26.55 CSV.dial CSR @12:07:27.12 Pulling 4ml of CSR.mfbkill @12:07:27.13 CSR.select :mfbkill @12:07:27.27 CS.reconfigure CSconfig @12:07:27.32 CS.seek 4ml @12:07:38.57 delay 5 seconds @12:07:43.60 CSR.select :air @12:07:43.73 CS.reconfigure CSfast @12:07:43.77 CS.seek 10ml @12:07:51.76 Priming Eluate @12:07:51.77 AFV.select :carrierSolution @12:07:51.88 delay 2 seconds @12:07:53.92 AS.seek 0.5ml @12:07:59.52 CSV.dial CTV @12:07:59.54
CTV.dial CSV @12:08:00.06 CBV.dial :waste2b @12:08:00.93 CS.seek 0ml @12:08:22.94 delay 1 second @12:08:23.96 AFV.select :holdingCoil @12:08:24.06 ATV.dial ASV @12:08:24.07 ASV.dial :SPErecovery @12:08:24.68 Swish #1 of 2 @12:08:24.76 AS.reconfigure ASslow0 @12:08:25.61 AS.seek 0.6ml @12:08:26.48
Killing upper manifold @12:08:26.49 CSV.dial CSR @12:08:27.07 Pulling 4ml of CSR.mfbkill @12:08:27.08 CSR.select :mfbkill @12:08:27.20 CS.reconfigure CSconfig @12:08:27.25 CS.seek 4ml @12:08:36.06 AS.seek 0.5ml @12:08:38.58
delay 5 seconds @12:08:43.61 CSR.select :air @12:08:43.73 CS.reconfigure CSfast @12:08:43.77 CS.seek 10ml @12:08:46.44 Swish #2 of 2 @12:08:46.52 AS.seek 0.6ml @12:08:57.00 AS.seek 0.5ml @12:08:59.73 CSV.dial CBV @12:08:59.76
CBV.dial CSV @12:09:00.97 CTV.dial :air @12:09:02.46 CS.seek 0ml @12:09:07.40 delay 3 seconds @12:09:10.49 AS.reconfigure ASslow1 @12:09:10.54 AS.seek 0.684ml @12:09:28.05
delay 7 seconds @12:09:35.09 CS.seek 3ml @12:09:43.42 CSV.dial :waste @12:09:43.91 CS.seek 0ml @12:09:47.85 delay 3 seconds @12:09:50.88 Eluate primed @12:09:50.89 ATV.dial :waste2 @12:09:51.51 AS.reconfigure ASnorm @12:09:51.57 AS.seek 0ml @12:09:52.20
Flushing upper manifold @12:09:52.34 CSV.dial :flush @12:09:52.85 CS.seek 5ml @12:10:06.11 delay 5 seconds @12:10:11.14 CSV.dial :air @12:10:11.71 CS.seek 7ml @12:10:17.64 CSV.dial CBV @12:10:17.68
CBV.dial CSV @12:10:18.84 CTV.dial :air @12:10:18.86 CS.seek 0ml @12:10:26.35 SPE.coast @12:10:26.43 ASV.dial :SPErecovery @12:10:26.45 Setting up PCR for OM -- writing /var/log/esp/OM20may2612h350ml.pcr @12:10:26.47 Building PCR Slug from ASV with MM3 mix, PP5 primer @12:10:26.48 Priming with carrier @12:10:26.49 ATV.dial ARV @12:10:27.45 ARV.dial :carrier @12:10:27.81 AS.seek 0.5ml @12:10:36.83
delay 7 seconds @12:10:43.87 CS.seek 3ml @12:10:52.02 CSV.dial :waste @12:10:52.53 CS.seek 0ml @12:10:53.37 AFV.select :carrierSolution @12:10:53.48 delay 2 seconds @12:10:55.51 AS.reconfigure ASconfig @12:10:55.56 AS.seek 1ml @12:11:00.79
CSV.dial :flush @12:11:01.28 CS.seek 3ml @12:11:09.48 delay 5 seconds @12:11:14.51 CSV.dial :air @12:11:15.07 CS.seek 5ml @12:11:20.83 CSV.dial :air @12:11:20.85 CS.seek 0ml @12:11:24.57 delay 1 second @12:11:25.59 AFV.select :holdingCoil @12:11:25.69 delay 1 second @12:11:26.72 ATV.dial :waste @12:11:28.16 AS.reconfigure ASnorm @12:11:28.22 AS.seek :empty @12:11:33.70
delay 7 seconds @12:11:40.74 CS.seek 3ml @12:11:48.99 CSV.dial :waste @12:11:49.81 CS.seek 0ml @12:11:57.99 Flushing lower manifold's waste1 port @12:11:58.13 CSV.dial :flush @12:11:58.64 CS.seek 5ml @12:12:12.21 delay 5 seconds @12:12:17.24 CSV.dial :air @12:12:17.93 CS.seek 7ml @12:12:18.96 AFV.select :carrierSolution @12:12:19.07 delay 2 seconds @12:12:21.10 AS.reconfigure ASconfig @12:12:21.15 AS.seek 0.5ml @12:12:23.74 CSV.dial CTV @12:12:23.77
CTV.dial CSV @12:12:25.31 CBV.dial :waste1 @12:12:25.82 CS.seek 0ml @12:12:43.36 Flushing lower manifold's waste2b port @12:12:43.50 CSV.dial :flush @12:12:44.34 CS.seek 5ml @12:12:50.27 delay 1 second @12:12:51.29 AFV.select :holdingCoil @12:12:51.39 Building Slug @12:12:51.40 ATV.dial :ARV @12:12:52.98 delay 2 seconds @12:12:55.01 ARV.dial :air1 @12:12:55.39 delay 2 seconds @12:12:57.33
delay 5 seconds @12:12:57.48 AS.reconfigure ASslow2 @12:12:57.54 AS.seek 0.506ml @12:13:02.35
CSV.dial :air @12:13:02.93 CS.seek 7.001ml @12:13:03.82 delay 2 seconds @12:13:05.85 ARV.dial :MM3 @12:13:06.87 delay 2 seconds @12:13:08.83 CSV.dial CTV @12:13:08.86
CTV.dial CSV @12:13:08.93 Adding MM3 @12:13:09.00 AS.seek 0.524ml @12:13:09.35
CBV.dial :waste2b @12:13:10.20 CS.seek 0ml @12:13:27.45 delay 25 seconds @12:13:27.83
Disinfecting external Intake with kill @12:13:27.97 CSV.dial CSR @12:13:28.53 Pulling 8ml of CSR.kill CSR.select :kill @12:13:28.66 CS.seek 8ml @12:13:48.71 delay 5 seconds @12:13:52.48 ARV.dial :PP5 @12:13:52.87 Adding PP5 @12:13:52.89 delay 2 seconds @12:13:53.73
CSR.select :air @12:13:53.86 CS.seek 10ml @12:13:54.97 AS.seek 0.53ml @12:13:59.61 CSV.dial CBV @12:13:59.64
CBV.dial CSV @12:14:00.82 CTV.dial :intake @12:14:01.15 delay 10 seconds @12:14:01.63
Intake.select :open @12:14:01.75 CS.seek 2ml @12:14:11.18 ARV.dial :air3 @12:14:11.59 adding ARV air @12:14:11.61 delay 2 seconds @12:14:13.69 AS.seek 0.536ml @12:14:20.04 delay 5 seconds @12:14:23.08
Intake.select :close @12:14:23.19 CTV.dial :air @12:14:24.00 CS.reconfigure CSconfig @12:14:24.05 CS.seek 0ml @12:14:25.06 ARV.dial :carrier @12:14:26.31 delay 7 seconds @12:14:30.22
CSV.dial CSR @12:14:31.39 Pulling 5ml of CSR.flush @12:14:31.40 CSR.select :flush @12:14:31.53 CS.seek 5ml @12:14:33.40 AS.seek 0.594ml @12:14:45.30
delay 5 seconds @12:14:50.33 CSR.select :air @12:14:50.46 CS.seek 10ml @12:15:04.27 CSV.dial CBV @12:15:05.39 CS.seek 0ml @12:15:31.99 Garaging FlushPuck in Collection Clamp @12:15:32.09 delay 5 seconds @12:15:32.11
Elbow.reconfigure TightElbow @12:15:32.92 Elbow.seek CC @12:15:33.18 Elbow.reconfigure LooseElbow @12:15:33.29 Forearm.seek CC @12:15:34.25 Hand.close @12:15:35.03 CC.seek :open @12:15:37.13 ATV.dial :ASV @12:15:37.54 delay 2 seconds @12:15:39.44
Forearm.seek :retract @12:15:39.63 AS.seek 0.6ml @12:15:40.44
Elbow.reconfigure TightElbow @12:15:40.49 Elbow.seek :garage @12:15:43.70 Elbow.reconfigure LooseElbow @12:15:43.80 Forearm.reconfigure TightForearm @12:15:43.85 Forearm.seek :garage @12:15:44.99 Hand.open @12:15:45.84 Forearm.reconfigure LooseForearm @12:15:45.89 Forearm.seek :retract @12:15:45.97 delay 4 seconds @12:15:46.92
Starting initialPurge @12:15:46.93 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @12:15:47.01 Hand.open @12:15:47.34 Elbow.reconfigure TightElbow @12:15:47.39 Elbow.seek FlushPuck::Garage @12:15:47.62 Elbow.reconfigure LooseElbow @12:15:47.73 Forearm.seek :garage @12:15:48.72 Hand.close @12:15:49.52 Forearm.seek :retract @12:15:49.99 ATV.dial ARV @12:15:50.54 delay 2 seconds @12:15:50.56
Elbow.reconfigure TightElbow @12:15:50.62 Elbow.seek CC @12:15:52.57 ARV.dial :carrier @12:15:52.59 delay 2 seconds @12:15:53.59
Elbow.reconfigure LooseElbow @12:15:53.69 Forearm.reconfigure TightForearm @12:15:53.75 Forearm.seek CC @12:15:54.67 AS.seek 0.65ml @12:15:55.08
CC.seek :closed @12:15:59.33 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.13 @12:15:59.41 Hand.open @12:16:00.21 Forearm.reconfigure LooseForearm @12:16:00.26 Forearm.seek :retract @12:16:01.28 Flushing the Collection Valve::Manifold @12:16:01.42 CSV.dial CSR @12:16:02.60 Pulling 5ml of CSR.flush @12:16:02.61 CSR.select :flush @12:16:02.74 CS.seek 5ml @12:16:16.52 delay 5 seconds @12:16:21.55 CSR.select :air @12:16:21.72 CS.reconfigure CSfast @12:16:21.77 CS.seek 7.5ml @12:16:28.83 CSV.dial :waste @12:16:30.26 CS.reconfigure CSconfig @12:16:30.31 CS.seek 0ml @12:16:45.10 delay 4 seconds @12:16:49.87 ATV.dial PCR @12:16:50.50 ASV.dial :waste1 @12:16:50.65
Purging external Intake @12:16:50.79 CSV.dial :flush @12:16:51.30 ARV.dial :air1 @12:16:51.37
CS.reconfigure CSfast @12:16:51.43 CS.seek 10ml @12:16:51.68 Positioning Slug by 0.324ml @12:16:51.77 AS.seek 0.326ml @12:17:16.18
delay 5 seconds @12:17:21.21 CSV.dial CBV @12:17:21.25
CBV.dial CSV @12:17:22.07 CTV.dial :intake @12:17:22.90 Intake.select :open @12:17:23.02 CS.seek 0ml @12:17:49.27 Intake.select :close @12:17:49.38 CTV.dial :air @12:17:50.21 Flushing upper manifold @12:17:50.35 CSV.dial :flush @12:17:51.17 CS.seek 5ml @12:18:03.99 delay 5 seconds @12:18:09.02 CSV.dial :air @12:18:09.70 CS.seek 7ml @12:18:15.40 CSV.dial CBV @12:18:15.43
CBV.dial CSV @12:18:16.56 CTV.dial :air @12:18:16.58 CS.seek 0ml @12:18:34.43 delay 7 seconds @12:18:41.47 CS.seek 3ml @12:18:49.71 CSV.dial :waste @12:18:50.21 CS.seek 0ml @12:18:58.43 CSV.dial :flush @12:18:58.93 CS.seek 3ml @12:19:07.03 delay 5 seconds @12:19:12.06 CSV.dial :air @12:19:12.74 CS.seek 5ml @12:19:18.62 CSV.dial :air @12:19:18.64 CS.seek 0ml @12:19:31.56 delay 7 seconds @12:19:38.60 CS.seek 3ml @12:19:46.99 CSV.dial :waste @12:19:47.79 CS.seek 0ml @12:19:56.06 Garaging FlushPuck in Collection Clamp @12:19:56.14 Elbow.reconfigure TightElbow @12:19:56.19 Elbow.seek CC @12:19:56.44 Elbow.reconfigure LooseElbow @12:19:56.54 Forearm.seek CC @12:19:57.53 Hand.close @12:19:58.28 CC.seek :open @12:20:02.66 Forearm.seek :retract @12:20:03.65 Elbow.reconfigure TightElbow @12:20:03.70 Elbow.seek :garage @12:20:06.87 Elbow.reconfigure LooseElbow @12:20:06.98 Forearm.reconfigure TightForearm @12:20:07.03 Forearm.seek :garage @12:20:08.17 Hand.open @12:20:08.97 Forearm.reconfigure LooseForearm @12:20:09.02 Forearm.seek :retract @12:20:10.06 Completed [stdphase] begun 10:02:36 @12:20:10.07 Preparing to sleep until 10:00 27-may-20 @12:20:10.13 deferred until MFBPCR finish @12:20:10.15
Waiting for MFBPCR til 10:00 27-may-20 @12:20:10.18 Continuing at 10:00CEST27-May-20 @12:20:10.24 GoodNews.email "Then, in tube 3: wcrphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:155\n{1 => 22, 2 => 21, 3 => 1}\nCan@12:20:10, 10.0C, 50% humidity, 14.4psia, 12.886V, 0.537A, 0.537A avg, 6.93W",Subject: "Waiting until 10:00CEST27-May-20" @12:22:17.90 delay 5 seconds @12:22:22.93 ATV.dial ARV @12:22:23.69 ASV.dial :PCRlock @12:22:24.06 delay 5 seconds @12:22:29.09 Running OM Profile60C350 thermocycling @12:22:29.10 PCR.program Profile60C350 @12:22:29.21 Profile60C350 should complete in < 2:02:45 @12:22:29.23 PCR.run 2:38:26.25 @13:59:06.76 PCR.off @13:59:08.03 PCR for OM completed -- closed /var/log/esp/OM20may2612h350ml.pcr @13:59:08.10 Cleaning PCR @13:59:08.11 AFV.select :carrierSolution @13:59:08.22 delay 2 seconds @13:59:10.25 AS.reconfigure ASconfig @13:59:10.30 AS.seek 0.5ml @13:59:20.59 delay 1 second @13:59:21.61 AFV.select :holdingCoil @13:59:21.71 ASV.dial :waste1 @13:59:22.10 ATV.dial :air3 @13:59:23.34 AS.reconfigure ASslow1 @13:59:23.39 AS.seek 0.51ml @13:59:25.66 delay 2 seconds @13:59:28.36 ATV.dial :bleach @13:59:29.06 AS.seek 0.81ml @14:00:29.65 delay 3 seconds @14:00:32.68 ATV.dial :air3 @14:00:33.21 delay 3 seconds @14:00:36.30 AS.seek 0.835ml @14:00:41.59 delay 3 seconds @14:00:44.62 ATV.dial :bleach @14:00:45.22 AS.seek 0.82ml @14:00:48.52 delay 3 seconds @14:00:51.55 ARV.dial :waste @14:00:52.15 delay 3 seconds @14:00:55.18 ATV.dial :ARV @14:00:56.05 AS.reconfigure ASconfig @14:00:56.10 AS.seek 0.47ml @14:01:16.52 AFV.select :carrierSolution @14:01:16.63 delay 2 seconds @14:01:18.67 AS.seek 1ml @14:01:49.48 delay 1 second @14:01:50.50 AFV.select :holdingCoil @14:01:50.60 delay 1 second @14:01:51.63 AS.reconfigure ASfast @14:01:51.68 AS.seek 0ml @14:02:19.04 AFV.select :carrierSolution @14:02:19.15 delay 2 seconds @14:02:21.18 AS.reconfigure ASconfig @14:02:21.23 AS.seek 1ml @14:03:18.96 delay 1 second @14:03:19.98 AFV.select :holdingCoil @14:03:20.08 delay 1 second @14:03:21.11 AS.reconfigure ASfast @14:03:21.16 AS.seek 0ml @14:03:48.49 AFV.select :carrierSolution @14:03:48.60 delay 2 seconds @14:03:50.63 AS.reconfigure ASconfig @14:03:50.68 AS.seek 1ml @14:04:48.45 delay 1 second @14:04:49.47 AFV.select :holdingCoil @14:04:49.57 delay 1 second @14:04:50.60 AS.reconfigure ASfast @14:04:50.65 AS.seek 0ml @14:05:18.00 ARV.dial between carrier and air1 @14:05:18.50 ATV.dial :air3 @14:05:19.74 AS.reconfigure ASslow1 @14:05:19.79 AS.seek 0.01ml @14:05:22.07 delay 2 seconds @14:05:24.09 ATV.dial :bleach @14:05:24.69 AS.seek 0.81ml @14:08:05.83 delay 3 seconds @14:08:08.86 ATV.dial :air3 @14:08:09.40 delay 3 seconds @14:08:12.49 AS.seek 0.835ml @14:08:17.79 delay 3 seconds @14:08:20.82 ATV.dial :bleach @14:08:21.43 AS.seek 0.82ml @14:08:24.69 delay 3 seconds @14:08:27.71 ATV.dial PCR @14:08:28.11 AS.reconfigure ASconfig @14:08:28.17 AS.seek :empty @14:09:15.61 PCR.program :bleachProfile @14:09:15.67 PCR program should complete in < 2:45 @14:09:15.68 PCR.run 10:00 @14:12:29.62 PCR.off @14:12:29.65 AFV.select :carrierSolution @14:12:29.76 delay 2 seconds @14:12:31.80 AS.seek 1ml @14:13:29.63 delay 1 second @14:13:30.65 AFV.select :holdingCoil @14:13:30.75 delay 1 second @14:13:31.78 AS.reconfigure ASfast @14:13:31.83 AS.seek 0ml @14:13:59.19 delay 3 seconds @14:14:02.22 AFV.select :carrierSolution @14:14:02.32 delay 2 seconds @14:14:04.35 AS.reconfigure ASconfig @14:14:04.40 AS.seek 1ml @14:15:02.14 delay 1 second @14:15:03.16 AFV.select :holdingCoil @14:15:03.26 delay 1 second @14:15:04.29 AS.reconfigure ASfast @14:15:04.34 AS.seek 0ml @14:15:31.66 delay 3 seconds @14:15:34.69 AFV.select :carrierSolution @14:15:34.79 delay 2 seconds @14:15:36.82 AS.reconfigure ASconfig @14:15:36.88 AS.seek 1ml @14:16:34.63 delay 1 second @14:16:35.65 AFV.select :holdingCoil @14:16:35.75 delay 1 second @14:16:36.78 AS.reconfigure ASfast @14:16:36.83 AS.seek 0ml @14:17:04.96 delay 3 seconds @14:17:07.99 AFV.select :carrierSolution @14:17:08.09 delay 2 seconds @14:17:10.12 AS.reconfigure ASconfig @14:17:10.18 AS.seek 1ml @14:18:07.97 delay 1 second @14:18:08.99 AFV.select :holdingCoil @14:18:09.09 delay 1 second @14:18:10.12 AS.reconfigure ASfast @14:18:10.17 AS.seek 0ml @14:18:37.52 delay 3 seconds @14:18:40.55 AFV.select :carrierSolution @14:18:40.65 delay 2 seconds @14:18:42.68 AS.reconfigure ASconfig @14:18:42.74 AS.seek 1ml @14:19:40.49 delay 1 second @14:19:41.51 AFV.select :holdingCoil @14:19:41.61 delay 1 second @14:19:42.64 AS.reconfigure ASnorm @14:19:42.69 AS.seek 0ml @14:20:33.40 Setting up PCR for ML -- writing /var/log/esp/ML20may2614h350ml.pcr @14:20:33.43 Building PCR Slug from ASV with MM2 mix, PP4 primer @14:20:33.44 Priming with carrier @14:20:33.45 ATV.dial ARV @14:20:34.15 ARV.dial :carrier @14:20:34.50 AS.seek 0.5ml @14:21:00.10 AFV.select :carrierSolution @14:21:00.21 delay 2 seconds @14:21:02.24 AS.reconfigure ASconfig @14:21:02.29 AS.seek 1ml @14:21:31.29 delay 1 second @14:21:32.31 AFV.select :holdingCoil @14:21:32.41 delay 1 second @14:21:33.44 ATV.dial :waste @14:21:35.02 AS.reconfigure ASnorm @14:21:35.08 AS.seek :empty @14:22:25.82 AFV.select :carrierSolution @14:22:25.93 delay 2 seconds @14:22:27.96 AS.reconfigure ASconfig @14:22:28.01 AS.seek 0.5ml @14:22:57.10 delay 1 second @14:22:58.12 AFV.select :holdingCoil @14:22:58.23 Building Slug @14:22:58.24 ATV.dial :ARV @14:22:59.82 delay 2 seconds @14:23:01.85 ARV.dial :air1 @14:23:02.24 delay 2 seconds @14:23:04.33 AS.reconfigure ASslow2 @14:23:04.38 AS.seek 0.506ml @14:23:10.66 delay 2 seconds @14:23:12.68 ARV.dial :MM2 @14:23:14.10 delay 2 seconds @14:23:16.13 Adding MM2 @14:23:16.22 AS.seek 0.524ml @14:23:34.66 delay 25 seconds @14:23:59.69 ARV.dial :PP4 @14:24:00.07 Adding PP4 @14:24:00.09 delay 2 seconds @14:24:02.17 AS.seek 0.53ml @14:24:08.37 delay 10 seconds @14:24:18.40 ARV.dial :air3 @14:24:18.79 adding ARV air @14:24:18.81 delay 2 seconds @14:24:20.90 AS.seek 0.536ml @14:24:27.12 delay 5 seconds @14:24:32.15 ARV.dial :carrier @14:24:33.37 delay 7 seconds @14:24:40.47 AS.seek 0.594ml @14:25:38.93 delay 5 seconds @14:25:43.96 ATV.dial :ASV @14:25:44.35 delay 2 seconds @14:25:46.44 AS.seek 0.6ml @14:25:52.73 delay 4 seconds @14:25:56.76 ATV.dial ARV @14:25:57.29 delay 2 seconds @14:25:59.32 ARV.dial :carrier @14:25:59.33 delay 2 seconds @14:26:01.42 AS.seek 0.65ml @14:26:51.95 delay 4 seconds @14:26:55.98 ATV.dial PCR @14:26:56.56 ASV.dial :waste1 @14:26:56.57 ARV.dial :air1 @14:26:56.97 Positioning Slug by 0.324ml @14:26:57.06 AS.seek 0.326ml @14:32:22.87 delay 5 seconds @14:32:27.90 ATV.dial ARV @14:32:28.65 ASV.dial :PCRlock @14:32:29.03 delay 5 seconds @14:32:34.06 Running ML Profile60C350 thermocycling @14:32:34.08 PCR.program Profile60C350 @14:32:34.18 Profile60C350 should complete in < 2:02:45 @14:32:34.20 PCR.run 2:38:26.25 @16:08:37.98 PCR.off @16:08:39.25 PCR for ML completed -- closed /var/log/esp/ML20may2614h350ml.pcr @16:08:39.32 Cleaning PCR @16:08:39.33 AFV.select :carrierSolution @16:08:39.44 delay 2 seconds @16:08:41.47 AS.reconfigure ASconfig @16:08:41.52 AS.seek 0.5ml @16:08:51.86 delay 1 second @16:08:52.88 AFV.select :holdingCoil @16:08:52.98 ASV.dial :waste1 @16:08:53.37 ATV.dial :air3 @16:08:54.63 AS.reconfigure ASslow1 @16:08:54.68 AS.seek 0.51ml @16:08:56.96 delay 2 seconds @16:08:58.98 ATV.dial :bleach @16:08:59.58 AS.seek 0.81ml @16:10:00.20 delay 3 seconds @16:10:03.23 ATV.dial :air3 @16:10:03.76 delay 3 seconds @16:10:06.85 AS.seek 0.835ml @16:10:12.21 delay 3 seconds @16:10:15.26 ATV.dial :bleach @16:10:15.86 AS.seek 0.82ml @16:10:19.15 delay 3 seconds @16:10:22.18 ARV.dial :waste @16:10:22.78 delay 3 seconds @16:10:25.81 ATV.dial :ARV @16:10:26.67 AS.reconfigure ASconfig @16:10:26.73 AS.seek 0.47ml @16:10:47.12 AFV.select :carrierSolution @16:10:47.23 delay 2 seconds @16:10:49.27 AS.seek 1ml @16:11:20.04 delay 1 second @16:11:21.06 AFV.select :holdingCoil @16:11:21.16 delay 1 second @16:11:22.19 AS.reconfigure ASfast @16:11:22.24 AS.seek 0ml @16:11:49.59 AFV.select :carrierSolution @16:11:49.70 delay 2 seconds @16:11:51.73 AS.reconfigure ASconfig @16:11:51.78 AS.seek 1ml @16:12:49.55 delay 1 second @16:12:50.57 AFV.select :holdingCoil @16:12:50.67 delay 1 second @16:12:52.45 AS.reconfigure ASfast @16:12:52.50 AS.seek 0ml @16:13:19.84 AFV.select :carrierSolution @16:13:19.95 delay 2 seconds @16:13:21.98 AS.reconfigure ASconfig @16:13:22.03 AS.seek 1ml @16:14:19.83 delay 1 second @16:14:20.85 AFV.select :holdingCoil @16:14:20.95 delay 1 second @16:14:21.98 AS.reconfigure ASfast @16:14:22.03 AS.seek 0ml @16:14:49.39 ARV.dial between carrier and air1 @16:14:49.89 ATV.dial :air3 @16:14:51.13 AS.reconfigure ASslow1 @16:14:51.18 AS.seek 0.01ml @16:14:53.48 delay 2 seconds @16:14:55.50 ATV.dial :bleach @16:14:56.10 AS.seek 0.81ml @16:17:37.24 delay 3 seconds @16:17:40.27 ATV.dial :air3 @16:17:40.81 delay 3 seconds @16:17:43.90 AS.seek 0.835ml @16:17:49.17 delay 3 seconds @16:17:52.20 ATV.dial :bleach @16:17:52.81 AS.seek 0.82ml @16:17:56.10 delay 3 seconds @16:17:59.13 ATV.dial PCR @16:17:59.53 AS.reconfigure ASconfig @16:17:59.58 AS.seek :empty @16:18:47.03 PCR.program :bleachProfile @16:18:47.09 PCR program should complete in < 2:45 @16:18:47.10 PCR.run 10:00 @16:22:01.04 PCR.off @16:22:01.06 AFV.select :carrierSolution @16:22:01.17 delay 2 seconds @16:22:03.21 AS.seek 1ml @16:23:01.04 delay 1 second @16:23:02.06 AFV.select :holdingCoil @16:23:02.16 delay 1 second @16:23:03.19 AS.reconfigure ASfast @16:23:03.24 AS.seek 0ml @16:23:30.58 delay 3 seconds @16:23:33.61 AFV.select :carrierSolution @16:23:33.71 delay 2 seconds @16:23:35.74 AS.reconfigure ASconfig @16:23:35.80 AS.seek 1ml @16:24:33.55 delay 1 second @16:24:34.57 AFV.select :holdingCoil @16:24:34.67 delay 1 second @16:24:35.70 AS.reconfigure ASfast @16:24:35.75 AS.seek 0ml @16:25:03.07 delay 3 seconds @16:25:06.10 AFV.select :carrierSolution @16:25:06.20 delay 2 seconds @16:25:08.24 AS.reconfigure ASconfig @16:25:08.29 AS.seek 1ml @16:26:06.10 delay 1 second @16:26:07.12 AFV.select :holdingCoil @16:26:07.22 delay 1 second @16:26:08.25 AS.reconfigure ASfast @16:26:08.30 AS.seek 0ml @16:26:35.66 delay 3 seconds @16:26:38.69 AFV.select :carrierSolution @16:26:38.79 delay 2 seconds @16:26:40.82 AS.reconfigure ASconfig @16:26:40.88 AS.seek 1ml @16:27:38.66 delay 1 second @16:27:39.68 AFV.select :holdingCoil @16:27:39.78 delay 1 second @16:27:40.81 AS.reconfigure ASfast @16:27:40.86 AS.seek 0ml @16:28:08.21 delay 3 seconds @16:28:11.24 AFV.select :carrierSolution @16:28:11.34 delay 2 seconds @16:28:13.37 AS.reconfigure ASconfig @16:28:13.42 AS.seek 1ml @16:29:11.17 delay 1 second @16:29:12.19 AFV.select :holdingCoil @16:29:12.29 delay 1 second @16:29:13.32 AS.reconfigure ASnorm @16:29:13.37 AS.seek 0ml @16:30:04.11 Setting up PCR for CG -- writing /var/log/esp/CG20may2616h350ml.pcr @16:30:04.13 Building PCR Slug from ASV with MM1 mix, PP2 primer @16:30:04.14 Priming with carrier @16:30:04.15 ATV.dial ARV @16:30:04.74 ARV.dial :carrier @16:30:05.10 AS.seek 0.5ml @16:30:30.67 AFV.select :carrierSolution @16:30:30.79 delay 2 seconds @16:30:32.82 AS.reconfigure ASconfig @16:30:32.87 AS.seek 1ml @16:31:01.89 delay 1 second @16:31:02.91 AFV.select :holdingCoil @16:31:03.01 delay 1 second @16:31:04.04 ATV.dial :waste @16:31:05.62 AS.reconfigure ASnorm @16:31:06.44 AS.seek :empty @16:31:57.20 AFV.select :carrierSolution @16:31:57.31 delay 2 seconds @16:31:59.34 AS.reconfigure ASconfig @16:31:59.39 AS.seek 0.5ml @16:32:28.46 delay 1 second @16:32:29.48 AFV.select :holdingCoil @16:32:29.58 Building Slug @16:32:29.59 ATV.dial :ARV @16:32:31.17 delay 2 seconds @16:32:33.20 ARV.dial :air1 @16:32:33.60 delay 2 seconds @16:32:35.69 AS.reconfigure ASslow2 @16:32:35.74 AS.seek 0.506ml @16:32:41.93 delay 2 seconds @16:32:43.96 ARV.dial :MM1 @16:32:44.54 delay 2 seconds @16:32:46.57 Adding MM1 @16:32:46.64 AS.seek 0.524ml @16:33:05.07 delay 25 seconds @16:33:30.10 ARV.dial :PP2 @16:33:30.48 Adding PP2 @16:33:30.50 delay 2 seconds @16:33:32.58 AS.seek 0.53ml @16:33:38.86 delay 10 seconds @16:33:48.89 ARV.dial :air2 @16:33:49.29 adding ARV air @16:33:49.31 delay 2 seconds @16:33:51.39 AS.seek 0.536ml @16:33:57.73 delay 5 seconds @16:34:02.76 ARV.dial :carrier @16:34:04.00 delay 7 seconds @16:34:11.09 AS.seek 0.594ml @16:35:09.79 delay 5 seconds @16:35:14.82 ATV.dial :ASV @16:35:15.22 delay 2 seconds @16:35:17.31 AS.seek 0.6ml @16:35:23.53 delay 4 seconds @16:35:27.56 ATV.dial ARV @16:35:28.09 delay 2 seconds @16:35:30.12 ARV.dial :carrier @16:35:30.13 delay 2 seconds @16:35:32.22 AS.seek 0.65ml @16:36:22.65 delay 4 seconds @16:36:26.68 ATV.dial PCR @16:36:27.26 ASV.dial :waste1 @16:36:27.27 ARV.dial :air1 @16:36:27.66 Positioning Slug by 0.324ml @16:36:27.75 AS.seek 0.326ml @16:41:53.88 delay 5 seconds @16:41:58.91 ATV.dial ARV @16:41:59.64 ASV.dial :PCRlock @16:42:00.03 delay 5 seconds @16:42:05.06 Running CG Profile60C300 thermocycling @16:42:05.08 PCR.program Profile60C300 @16:42:05.18 Profile60C300 should complete in < 1:59:25 @16:42:05.19 PCR.run 2:34:16.25 @18:17:11.92 PCR.off @18:17:12.72 PCR for CG completed -- closed /var/log/esp/CG20may2616h350ml.pcr @18:17:12.79 Cleaning PCR @18:17:12.80 AFV.select :carrierSolution @18:17:12.91 delay 2 seconds @18:17:14.99 AS.reconfigure ASconfig @18:17:15.03 AS.seek 0.5ml @18:17:25.39 delay 1 second @18:17:26.41 AFV.select :holdingCoil @18:17:26.51 ASV.dial :waste1 @18:17:26.89 ATV.dial :air3 @18:17:28.13 AS.reconfigure ASslow1 @18:17:28.18 AS.seek 0.51ml @18:17:30.45 delay 2 seconds @18:17:32.47 ATV.dial :bleach @18:17:33.07 AS.seek 0.81ml @18:18:33.67 delay 3 seconds @18:18:36.70 ATV.dial :air3 @18:18:37.24 delay 3 seconds @18:18:40.33 AS.seek 0.835ml @18:18:45.63 delay 3 seconds @18:18:48.66 ATV.dial :bleach @18:18:49.26 AS.seek 0.82ml @18:18:52.50 delay 3 seconds @18:18:55.53 ARV.dial :waste @18:18:56.13 delay 3 seconds @18:18:59.16 ATV.dial :ARV @18:19:00.02 AS.reconfigure ASconfig @18:19:00.07 AS.seek 0.47ml @18:19:20.47 AFV.select :carrierSolution @18:19:20.58 delay 2 seconds @18:19:22.62 AS.seek 1ml @18:19:53.39 delay 1 second @18:19:54.41 AFV.select :holdingCoil @18:19:54.51 delay 1 second @18:19:55.54 AS.reconfigure ASfast @18:19:55.59 AS.seek 0ml @18:20:22.94 AFV.select :carrierSolution @18:20:23.05 delay 2 seconds @18:20:25.08 AS.reconfigure ASconfig @18:20:25.13 AS.seek 1ml @18:21:22.97 delay 1 second @18:21:23.99 AFV.select :holdingCoil @18:21:24.09 delay 1 second @18:21:25.12 AS.reconfigure ASfast @18:21:25.17 AS.seek 0ml @18:21:52.52 AFV.select :carrierSolution @18:21:52.63 delay 2 seconds @18:21:54.66 AS.reconfigure ASconfig @18:21:54.71 AS.seek 1ml @18:22:52.52 delay 1 second @18:22:53.54 AFV.select :holdingCoil @18:22:53.64 delay 1 second @18:22:54.67 AS.reconfigure ASfast @18:22:54.72 AS.seek 0ml @18:23:22.07 ARV.dial between carrier and air1 @18:23:22.57 ATV.dial :air3 @18:23:23.81 AS.reconfigure ASslow1 @18:23:23.86 AS.seek 0.01ml @18:23:26.14 delay 2 seconds @18:23:28.16 ATV.dial :bleach @18:23:28.76 AS.seek 0.81ml @18:26:09.88 delay 3 seconds @18:26:12.90 ATV.dial :air3 @18:26:13.44 delay 3 seconds @18:26:16.53 AS.seek 0.835ml @18:26:21.86 delay 3 seconds @18:26:24.89 ATV.dial :bleach @18:26:25.50 AS.seek 0.82ml @18:26:28.76 delay 3 seconds @18:26:31.78 ATV.dial PCR @18:26:32.18 AS.reconfigure ASconfig @18:26:32.23 AS.seek :empty @18:27:19.71 PCR.program :bleachProfile @18:27:19.77 PCR program should complete in < 2:45 @18:27:19.78 PCR.run 10:00 @18:30:31.45 PCR.off @18:30:31.47 AFV.select :carrierSolution @18:30:31.58 delay 2 seconds @18:30:33.62 AS.seek 1ml @18:31:31.43 delay 1 second @18:31:32.45 AFV.select :holdingCoil @18:31:32.55 delay 1 second @18:31:33.58 AS.reconfigure ASfast @18:31:33.63 AS.seek 0ml @18:32:00.98 delay 3 seconds @18:32:04.01 AFV.select :carrierSolution @18:32:04.11 delay 2 seconds @18:32:06.14 AS.reconfigure ASconfig @18:32:06.20 AS.seek 1ml @18:33:03.98 delay 1 second @18:33:05.00 AFV.select :holdingCoil @18:33:05.10 delay 1 second @18:33:06.13 AS.reconfigure ASfast @18:33:06.18 AS.seek 0ml @18:33:33.53 delay 3 seconds @18:33:36.56 AFV.select :carrierSolution @18:33:36.66 delay 2 seconds @18:33:38.69 AS.reconfigure ASconfig @18:33:38.75 AS.seek 1ml @18:34:36.51 delay 1 second @18:34:37.53 AFV.select :holdingCoil @18:34:37.63 delay 1 second @18:34:38.66 AS.reconfigure ASfast @18:34:38.71 AS.seek 0ml @18:35:06.07 delay 3 seconds @18:35:09.10 AFV.select :carrierSolution @18:35:09.20 delay 2 seconds @18:35:11.23 AS.reconfigure ASconfig @18:35:11.29 AS.seek 1ml @18:36:09.11 delay 1 second @18:36:10.13 AFV.select :holdingCoil @18:36:10.23 delay 1 second @18:36:11.26 AS.reconfigure ASfast @18:36:11.31 AS.seek 0ml @18:36:38.67 delay 3 seconds @18:36:41.70 AFV.select :carrierSolution @18:36:41.80 delay 2 seconds @18:36:43.83 AS.reconfigure ASconfig @18:36:43.89 AS.seek 1ml @18:37:41.70 delay 1 second @18:37:42.72 AFV.select :holdingCoil @18:37:42.82 delay 1 second @18:37:43.85 AS.reconfigure ASnorm @18:37:43.90 AS.seek 0ml @18:38:34.61 Setting up PCR for NM -- writing /var/log/esp/NM20may2618h350ml.pcr @18:38:34.65 Building PCR Slug from ASV with MM1 mix, PP1 primer @18:38:34.66 Priming with carrier @18:38:34.67 ATV.dial ARV @18:38:35.26 ARV.dial :carrier @18:38:35.62 AS.seek 0.5ml @18:39:01.22 AFV.select :carrierSolution @18:39:01.33 delay 2 seconds @18:39:03.36 AS.reconfigure ASconfig @18:39:03.41 AS.seek 1ml @18:39:32.41 delay 1 second @18:39:33.43 AFV.select :holdingCoil @18:39:33.53 delay 1 second @18:39:34.56 ATV.dial :waste @18:39:36.14 AS.reconfigure ASnorm @18:39:36.19 AS.seek :empty @18:40:26.92 AFV.select :carrierSolution @18:40:27.03 delay 2 seconds @18:40:29.06 AS.reconfigure ASconfig @18:40:29.11 AS.seek 0.5ml @18:40:58.22 delay 1 second @18:40:59.24 AFV.select :holdingCoil @18:40:59.34 Building Slug @18:40:59.35 ATV.dial :ARV @18:41:00.92 delay 2 seconds @18:41:02.95 ARV.dial :air1 @18:41:03.34 delay 2 seconds @18:41:05.43 AS.reconfigure ASslow2 @18:41:05.48 AS.seek 0.506ml @18:41:11.78 delay 2 seconds @18:41:13.80 ARV.dial :MM1 @18:41:14.39 delay 2 seconds @18:41:16.42 Adding MM1 @18:41:16.49 AS.seek 0.524ml @18:41:35.00 delay 25 seconds @18:42:00.03 ARV.dial :PP1 @18:42:00.41 Adding PP1 @18:42:00.43 delay 2 seconds @18:42:02.51 AS.seek 0.53ml @18:42:09.02 delay 10 seconds @18:42:19.05 ARV.dial :air1 @18:42:19.44 adding ARV air @18:42:19.45 delay 2 seconds @18:42:21.54 AS.seek 0.536ml @18:42:27.94 delay 5 seconds @18:42:32.97 ARV.dial :carrier @18:42:33.36 delay 7 seconds @18:42:40.45 AS.seek 0.594ml @18:43:39.14 delay 5 seconds @18:43:44.17 ATV.dial :ASV @18:43:44.56 delay 2 seconds @18:43:46.65 AS.seek 0.6ml @18:43:52.89 delay 4 seconds @18:43:56.92 ATV.dial ARV @18:43:57.46 delay 2 seconds @18:43:59.49 ARV.dial :carrier @18:44:00.25 delay 2 seconds @18:44:02.36 AS.seek 0.65ml @18:44:52.91 delay 4 seconds @18:44:56.94 ATV.dial PCR @18:44:57.52 ASV.dial :waste1 @18:44:57.54 ARV.dial :air1 @18:44:57.93 Positioning Slug by 0.324ml @18:44:58.02 AS.seek 0.326ml @18:50:23.90 delay 5 seconds @18:50:28.93 ATV.dial ARV @18:50:29.80 ASV.dial :PCRlock @18:50:30.19 delay 5 seconds @18:50:35.22 Running NM Profile60C300 thermocycling @18:50:35.23 PCR.program Profile60C300 @18:50:35.34 Profile60C300 should complete in < 1:59:25 @18:50:35.35 PCR.run 2:34:16.25 @20:25:35.29 PCR.off @20:25:35.95 PCR for NM completed -- closed /var/log/esp/NM20may2618h350ml.pcr @20:25:36.02 Cleaning PCR @20:25:36.03 AFV.select :carrierSolution @20:25:36.14 delay 2 seconds @20:25:38.17 AS.reconfigure ASconfig @20:25:38.22 AS.seek 0.5ml @20:25:48.52 delay 1 second @20:25:49.54 AFV.select :holdingCoil @20:25:49.64 ASV.dial :waste1 @20:25:50.02 ATV.dial :air3 @20:25:51.25 AS.reconfigure ASslow1 @20:25:51.30 AS.seek 0.51ml @20:25:53.58 delay 2 seconds @20:25:55.60 ATV.dial :bleach @20:25:56.20 AS.seek 0.81ml @20:26:56.87 delay 3 seconds @20:26:59.90 ATV.dial :air3 @20:27:00.44 delay 3 seconds @20:27:03.53 AS.seek 0.835ml @20:27:08.84 delay 3 seconds @20:27:11.87 ATV.dial :bleach @20:27:12.48 AS.seek 0.82ml @20:27:15.73 delay 3 seconds @20:27:18.76 ARV.dial :waste @20:27:19.35 delay 3 seconds @20:27:22.38 ATV.dial :ARV @20:27:23.25 AS.reconfigure ASconfig @20:27:23.30 AS.seek 0.47ml @20:27:44.48 AFV.select :carrierSolution @20:27:44.59 delay 2 seconds @20:27:46.63 AS.seek 1ml @20:28:17.35 delay 1 second @20:28:18.37 AFV.select :holdingCoil @20:28:18.47 delay 1 second @20:28:19.50 AS.reconfigure ASfast @20:28:19.55 AS.seek 0ml @20:28:46.88 AFV.select :carrierSolution @20:28:46.99 delay 2 seconds @20:28:49.02 AS.reconfigure ASconfig @20:28:49.07 AS.seek 1ml @20:29:46.91 delay 1 second @20:29:47.93 AFV.select :holdingCoil @20:29:48.03 delay 1 second @20:29:49.06 AS.reconfigure ASfast @20:29:49.11 AS.seek 0ml @20:30:16.46 AFV.select :carrierSolution @20:30:16.57 delay 2 seconds @20:30:18.60 AS.reconfigure ASconfig @20:30:18.65 AS.seek 1ml @20:31:16.49 delay 1 second @20:31:17.51 AFV.select :holdingCoil @20:31:17.61 delay 1 second @20:31:18.64 AS.reconfigure ASfast @20:31:18.69 AS.seek 0ml @20:31:46.04 ARV.dial between carrier and air1 @20:31:46.54 ATV.dial :air3 @20:31:47.78 AS.reconfigure ASslow1 @20:31:47.83 AS.seek 0.01ml @20:31:50.13 delay 2 seconds @20:31:52.15 ATV.dial :bleach @20:31:52.75 AS.seek 0.81ml @20:34:33.89 delay 3 seconds @20:34:36.92 ATV.dial :air3 @20:34:37.46 delay 3 seconds @20:34:40.55 AS.seek 0.835ml @20:34:45.83 delay 3 seconds @20:34:48.85 ATV.dial :bleach @20:34:49.46 AS.seek 0.82ml @20:34:52.76 delay 3 seconds @20:34:55.79 ATV.dial PCR @20:34:56.18 AS.reconfigure ASconfig @20:34:56.23 AS.seek :empty @20:35:43.70 PCR.program :bleachProfile @20:35:43.76 PCR program should complete in < 2:45 @20:35:43.77 PCR.run 10:00 @20:38:57.71 PCR.off @20:38:57.74 AFV.select :carrierSolution @20:38:57.85 delay 2 seconds @20:38:59.89 AS.seek 1ml @20:39:57.72 delay 1 second @20:39:58.74 AFV.select :holdingCoil @20:39:58.84 delay 1 second @20:39:59.87 AS.reconfigure ASfast @20:39:59.92 AS.seek 0ml @20:40:27.28 delay 3 seconds @20:40:30.31 AFV.select :carrierSolution @20:40:30.41 delay 2 seconds @20:40:32.44 AS.reconfigure ASconfig @20:40:32.49 AS.seek 1ml @20:41:30.27 delay 1 second @20:41:31.29 AFV.select :holdingCoil @20:41:31.39 delay 1 second @20:41:32.42 AS.reconfigure ASfast @20:41:32.47 AS.seek 0ml @20:41:59.83 delay 3 seconds @20:42:02.86 AFV.select :carrierSolution @20:42:02.96 delay 2 seconds @20:42:04.99 AS.reconfigure ASconfig @20:42:05.04 AS.seek 1ml @20:43:02.80 delay 1 second @20:43:03.82 AFV.select :holdingCoil @20:43:03.92 delay 1 second @20:43:04.95 AS.reconfigure ASfast @20:43:05.00 AS.seek 0ml @20:43:32.35 delay 3 seconds @20:43:35.38 AFV.select :carrierSolution @20:43:35.48 delay 2 seconds @20:43:37.51 AS.reconfigure ASconfig @20:43:37.56 AS.seek 1ml @20:44:35.31 delay 1 second @20:44:36.33 AFV.select :holdingCoil @20:44:36.43 delay 1 second @20:44:37.46 AS.reconfigure ASfast @20:44:37.51 AS.seek 0ml @20:45:04.85 delay 3 seconds @20:45:07.88 AFV.select :carrierSolution @20:45:07.98 delay 2 seconds @20:45:10.01 AS.reconfigure ASconfig @20:45:10.06 AS.seek 1ml @20:46:07.82 delay 1 second @20:46:08.84 AFV.select :holdingCoil @20:46:08.94 delay 1 second @20:46:09.97 AS.reconfigure ASnorm @20:46:10.02 AS.seek 0ml @20:47:00.76 Setting up PCR for IPc -- writing /var/log/esp/IPc20may2620h350ml.pcr @20:47:00.77 Building PCR Slug from ASV with MM3 mix, PP6 primer @20:47:00.79 Priming with carrier @20:47:00.80 ATV.dial ARV @20:47:01.49 ARV.dial :carrier @20:47:02.61 AS.seek 0.5ml @20:47:28.24 AFV.select :carrierSolution @20:47:28.35 delay 2 seconds @20:47:30.38 AS.reconfigure ASconfig @20:47:30.43 AS.seek 1ml @20:47:59.46 delay 1 second @20:48:00.48 AFV.select :holdingCoil @20:48:00.58 delay 1 second @20:48:01.61 ATV.dial :waste @20:48:03.19 AS.reconfigure ASnorm @20:48:03.25 AS.seek :empty @20:48:53.99 AFV.select :carrierSolution @20:48:54.10 delay 2 seconds @20:48:56.13 AS.reconfigure ASconfig @20:48:56.18 AS.seek 0.5ml @20:49:25.26 delay 1 second @20:49:26.28 AFV.select :holdingCoil @20:49:26.38 Building Slug @20:49:26.39 ATV.dial :ARV @20:49:27.96 delay 2 seconds @20:49:29.99 ARV.dial :air1 @20:49:30.37 delay 2 seconds @20:49:32.46 AS.reconfigure ASslow2 @20:49:32.51 AS.seek 0.506ml @20:49:38.71 delay 2 seconds @20:49:40.73 ARV.dial :MM3 @20:49:41.76 delay 2 seconds @20:49:43.79 Adding MM3 @20:49:43.87 AS.seek 0.524ml @20:50:02.15 delay 25 seconds @20:50:27.18 ARV.dial :PP6 @20:50:27.57 Adding PP6 @20:50:27.59 delay 2 seconds @20:50:29.67 AS.seek 0.53ml @20:50:36.12 delay 10 seconds @20:50:46.15 ARV.dial :air1 @20:50:46.96 adding ARV air @20:50:46.97 delay 2 seconds @20:50:49.06 AS.seek 0.536ml @20:50:55.47 delay 5 seconds @20:51:00.50 ARV.dial :carrier @20:51:00.91 delay 7 seconds @20:51:08.00 AS.seek 0.594ml @20:52:06.66 delay 5 seconds @20:52:11.69 ATV.dial :ASV @20:52:12.09 delay 2 seconds @20:52:14.18 AS.seek 0.6ml @20:52:20.49 delay 4 seconds @20:52:24.52 ATV.dial ARV @20:52:25.05 delay 2 seconds @20:52:27.08 ARV.dial :carrier @20:52:27.09 delay 2 seconds @20:52:29.18 AS.seek 0.65ml @20:53:19.84 delay 4 seconds @20:53:23.87 ATV.dial PCR @20:53:24.46 ASV.dial :waste1 @20:53:24.47 ARV.dial :air1 @20:53:24.86 Positioning Slug by 0.324ml @20:53:24.95 AS.seek 0.326ml @20:58:51.21 delay 5 seconds @20:58:56.24 ATV.dial ARV @20:58:57.09 ASV.dial :PCRlock @20:58:57.48 delay 5 seconds @20:59:02.51 Running IPc ProfileIPc thermocycling @20:59:02.52 PCR.program ProfileIPc @20:59:02.63 ProfileIPc should complete in < 1:36:15 @20:59:02.64 PCR.run 2:05:18.75 @22:17:06.56 PCR.off @22:17:07.23 PCR for IPc completed -- closed /var/log/esp/IPc20may2620h350ml.pcr @22:17:07.30 Cleaning PCR @22:17:07.31 AFV.select :carrierSolution @22:17:07.42 delay 2 seconds @22:17:09.51 AS.reconfigure ASconfig @22:17:09.55 AS.seek 0.5ml @22:17:19.84 delay 1 second @22:17:20.86 AFV.select :holdingCoil @22:17:20.96 ASV.dial :waste1 @22:17:21.34 ATV.dial :air3 @22:17:22.57 AS.reconfigure ASslow1 @22:17:22.62 AS.seek 0.51ml @22:17:24.89 delay 2 seconds @22:17:26.91 ATV.dial :bleach @22:17:27.54 AS.seek 0.81ml @22:18:28.14 delay 3 seconds @22:18:31.17 ATV.dial :air3 @22:18:31.70 delay 3 seconds @22:18:34.79 AS.seek 0.835ml @22:18:40.09 delay 3 seconds @22:18:43.12 ATV.dial :bleach @22:18:43.73 AS.seek 0.82ml @22:18:46.95 delay 3 seconds @22:18:49.98 ARV.dial :waste @22:18:50.56 delay 3 seconds @22:18:53.59 ATV.dial :ARV @22:18:54.45 AS.reconfigure ASconfig @22:18:54.51 AS.seek 0.47ml @22:19:14.94 AFV.select :carrierSolution @22:19:15.05 delay 2 seconds @22:19:17.09 AS.seek 1ml @22:19:47.85 delay 1 second @22:19:48.87 AFV.select :holdingCoil @22:19:48.97 delay 1 second @22:19:50.00 AS.reconfigure ASfast @22:19:50.05 AS.seek 0ml @22:20:17.39 AFV.select :carrierSolution @22:20:17.50 delay 2 seconds @22:20:19.53 AS.reconfigure ASconfig @22:20:19.58 AS.seek 1ml @22:21:17.39 delay 1 second @22:21:18.41 AFV.select :holdingCoil @22:21:18.51 delay 1 second @22:21:19.54 AS.reconfigure ASfast @22:21:19.59 AS.seek 0ml @22:21:46.94 AFV.select :carrierSolution @22:21:47.05 delay 2 seconds @22:21:49.08 AS.reconfigure ASconfig @22:21:49.13 AS.seek 1ml @22:22:46.93 delay 1 second @22:22:47.95 AFV.select :holdingCoil @22:22:48.05 delay 1 second @22:22:49.08 AS.reconfigure ASfast @22:22:49.13 AS.seek 0ml @22:23:16.50 ARV.dial between carrier and air1 @22:23:16.97 ATV.dial :air3 @22:23:18.20 AS.reconfigure ASslow1 @22:23:19.02 AS.seek 0.01ml @22:23:21.30 delay 2 seconds @22:23:23.32 ATV.dial :bleach @22:23:23.93 AS.seek 0.81ml @22:26:05.07 delay 3 seconds @22:26:08.10 ATV.dial :air3 @22:26:08.63 delay 3 seconds @22:26:11.72 AS.seek 0.835ml @22:26:17.02 delay 3 seconds @22:26:20.05 ATV.dial :bleach @22:26:20.65 AS.seek 0.82ml @22:26:23.93 delay 3 seconds @22:26:26.96 ATV.dial PCR @22:26:27.35 AS.reconfigure ASconfig @22:26:27.40 AS.seek :empty @22:27:14.85 PCR.program :bleachProfile @22:27:14.91 PCR program should complete in < 2:45 @22:27:14.92 PCR.run 10:00 @22:30:28.86 PCR.off @22:30:28.88 AFV.select :carrierSolution @22:30:28.99 delay 2 seconds @22:30:31.03 AS.seek 1ml @22:31:28.82 delay 1 second @22:31:29.84 AFV.select :holdingCoil @22:31:29.94 delay 1 second @22:31:30.97 AS.reconfigure ASfast @22:31:31.02 AS.seek 0ml @22:31:58.37 delay 3 seconds @22:32:01.40 AFV.select :carrierSolution @22:32:01.50 delay 2 seconds @22:32:03.53 AS.reconfigure ASconfig @22:32:03.58 AS.seek 1ml @22:33:01.35 delay 1 second @22:33:02.37 AFV.select :holdingCoil @22:33:02.47 delay 1 second @22:33:03.50 AS.reconfigure ASfast @22:33:03.55 AS.seek 0ml @22:33:30.90 delay 3 seconds @22:33:33.93 AFV.select :carrierSolution @22:33:34.03 delay 2 seconds @22:33:36.06 AS.reconfigure ASconfig @22:33:36.12 AS.seek 1ml @22:34:33.90 delay 1 second @22:34:34.92 AFV.select :holdingCoil @22:34:35.02 delay 1 second @22:34:36.05 AS.reconfigure ASfast @22:34:36.10 AS.seek 0ml @22:35:03.45 delay 3 seconds @22:35:06.48 AFV.select :carrierSolution @22:35:06.58 delay 2 seconds @22:35:08.61 AS.reconfigure ASconfig @22:35:08.66 AS.seek 1ml @22:36:06.45 delay 1 second @22:36:07.47 AFV.select :holdingCoil @22:36:07.57 delay 1 second @22:36:08.60 AS.reconfigure ASfast @22:36:08.65 AS.seek 0ml @22:36:36.01 delay 3 seconds @22:36:39.04 AFV.select :carrierSolution @22:36:39.14 delay 2 seconds @22:36:41.17 AS.reconfigure ASconfig @22:36:41.23 AS.seek 1ml @22:37:39.05 delay 1 second @22:37:40.07 AFV.select :holdingCoil @22:37:40.17 delay 1 second @22:37:41.20 AS.reconfigure ASnorm @22:37:41.25 AS.seek 0ml @22:38:31.97 Setting up PCR for IPc2 -- writing /var/log/esp/IPc220may2622h350ml.pcr @22:38:32.01 Building PCR Slug from ASV with MM2 mix, PP3 primer @22:38:32.02 Priming with carrier @22:38:32.03 ATV.dial ARV @22:38:32.73 ARV.dial :carrier @22:38:33.09 AS.seek 0.5ml @22:38:58.70 AFV.select :carrierSolution @22:38:58.81 delay 2 seconds @22:39:00.84 AS.reconfigure ASconfig @22:39:00.89 AS.seek 1ml @22:39:29.92 delay 1 second @22:39:30.94 AFV.select :holdingCoil @22:39:31.04 delay 1 second @22:39:32.07 ATV.dial :waste @22:39:33.66 AS.reconfigure ASnorm @22:39:33.72 AS.seek :empty @22:40:24.48 AFV.select :carrierSolution @22:40:24.59 delay 2 seconds @22:40:26.62 AS.reconfigure ASconfig @22:40:26.67 AS.seek 0.5ml @22:40:55.75 delay 1 second @22:40:56.77 AFV.select :holdingCoil @22:40:56.87 Building Slug @22:40:56.88 ATV.dial :ARV @22:40:58.45 delay 2 seconds @22:41:00.48 ARV.dial :air1 @22:41:00.87 delay 2 seconds @22:41:02.95 AS.reconfigure ASslow2 @22:41:03.01 AS.seek 0.506ml @22:41:09.23 delay 2 seconds @22:41:12.02 ARV.dial :MM2 @22:41:13.45 delay 2 seconds @22:41:15.48 Adding MM2 @22:41:15.55 AS.seek 0.524ml @22:41:33.97 delay 25 seconds @22:41:59.00 ARV.dial :PP3 @22:41:59.38 Adding PP3 @22:41:59.40 delay 2 seconds @22:42:01.48 AS.seek 0.53ml @22:42:07.77 delay 10 seconds @22:42:17.80 ARV.dial :air2 @22:42:18.19 adding ARV air @22:42:18.21 delay 2 seconds @22:42:20.29 AS.seek 0.536ml @22:42:26.47 delay 5 seconds @22:42:31.50 ARV.dial :carrier @22:42:32.74 delay 7 seconds @22:42:39.83 AS.seek 0.594ml @22:43:38.30 delay 5 seconds @22:43:43.33 ATV.dial :ASV @22:43:43.72 delay 2 seconds @22:43:45.81 AS.seek 0.6ml @22:43:52.05 delay 4 seconds @22:43:56.08 ATV.dial ARV @22:43:56.61 delay 2 seconds @22:43:58.64 ARV.dial :carrier @22:43:58.65 delay 2 seconds @22:44:00.74 AS.seek 0.65ml @22:44:51.39 delay 4 seconds @22:44:55.42 ATV.dial PCR @22:44:56.00 ASV.dial :waste1 @22:44:56.01 ARV.dial :air1 @22:44:56.40 Positioning Slug by 0.324ml @22:44:56.49 AS.seek 0.326ml @22:50:22.54 delay 5 seconds @22:50:27.57 ATV.dial ARV @22:50:28.32 ASV.dial :PCRlock @22:50:28.71 delay 5 seconds @22:50:33.74 Running IPc2 ProfileIPc thermocycling @22:50:33.76 PCR.program ProfileIPc @22:50:33.86 ProfileIPc should complete in < 1:36:15 @22:50:33.87 PCR.run 2:05:18.75 @00:08:42.22CEST27-May-20 PCR.off @00:08:42.79 PCR for IPc2 completed -- closed /var/log/esp/IPc220may2622h350ml.pcr @00:08:42.81 Full MFB Decontamination @00:08:42.82 Decon extraction column and decon AIV @00:08:42.84 SPE.hold 2:46:40,55C @00:08:42.89 ASV.dial :SPEdelivery @00:08:43.56 delay 20 seconds @00:09:03.59 AFV.select :carrierSolution @00:09:03.69 delay 2 seconds @00:09:05.72 AS.reconfigure ASconfig @00:09:05.77 AS.seek 0.5ml @00:09:16.07 delay 1 second @00:09:17.09 AFV.select :holdingCoil @00:09:17.19 ATV.dial :air1 @00:09:19.14 AS.reconfigure ASslow1 @00:09:19.19 AS.seek 0.52ml @00:09:23.40 ATV.dial :ASV @00:09:25.17 ASV.dial :SPErecovery @00:09:25.86 AS.seek 0.62ml @00:09:46.25 ATV.dial :waste @00:09:48.26 AS.reconfigure ASnorm @00:09:48.31 AS.seek :empty @00:10:19.93 AFV.select :holdingCoil @00:10:19.94 ATV.dial :waste @00:10:19.96 AS.reconfigure ASconfig @00:10:20.01 AS.seek 0ml @00:10:20.29 AFV.select :carrierSolution @00:10:20.40 delay 2 seconds @00:10:22.44 AS.seek 0.5ml @00:10:51.45 delay 1 second @00:10:52.47 AFV.select :holdingCoil @00:10:52.57 ATV.dial :air3 @00:10:53.30 AS.reconfigure ASslow1 @00:10:53.35 AS.seek 0.52ml @00:10:57.67 ATV.dial :air3 @00:10:57.76 AS.seek 0.53ml @00:11:00.03 delay 2 seconds @00:11:02.05 ATV.dial :bleach @00:11:02.54 AS.seek 0.71ml @00:11:39.01 delay 3 seconds @00:11:42.04 ATV.dial :air3 @00:11:42.62 delay 3 seconds @00:11:45.71 AS.seek 0.735ml @00:11:50.93 delay 3 seconds @00:11:53.96 ATV.dial :bleach @00:11:54.61 AS.seek 0.72ml @00:11:57.86 delay 3 seconds @00:12:00.89 ATV.dial ASV @00:12:02.00 ASV.dial :SPErecovery @00:12:02.09 AS.seek 0.365ml @00:13:13.72 ATV.dial :waste @00:13:15.47 AS.reconfigure ASnorm @00:13:15.52 AS.seek :empty @00:13:34.31 ATV.dial :air3 @00:13:35.02 AS.reconfigure ASslow1 @00:13:35.07 AS.seek 0.009ml @00:13:37.34 delay 2 seconds @00:13:39.36 ATV.dial :bleach @00:13:39.83 AS.seek 0.579ml @00:15:34.66 delay 3 seconds @00:15:37.69 ATV.dial :air3 @00:15:38.25 delay 3 seconds @00:15:41.34 AS.seek 0.604ml @00:15:46.62 delay 3 seconds @00:15:49.64 ATV.dial :bleach @00:15:50.26 AS.seek 0.589ml @00:15:53.53 delay 3 seconds @00:15:56.56 ATV.dial :ASV @00:15:57.59 ASV.dial :wasteR @00:15:58.85 AS.seek 0ml @00:17:57.63 delay 1:00 @00:18:57.66 ATV.dial ASV @00:18:57.67 AFV.select :carrierSolution @00:18:57.77 delay 2 seconds @00:18:59.80 AS.reconfigure ASconfig @00:18:59.85 AS.seek 1ml @00:19:57.63 delay 1 second @00:19:58.65 AFV.select :holdingCoil @00:19:58.75 delay 1 second @00:19:59.78 ASV.dial :wasteR @00:19:59.80 AS.reconfigure ASslow1 @00:19:59.84 AS.seek 0ml @00:23:21.21 delay 10 seconds @00:23:31.24 ATV.dial :air3 @00:23:32.73 AS.seek 0.01ml @00:23:35.02 delay 2 seconds @00:23:37.04 ATV.dial :bleach @00:23:37.68 AS.seek 0.58ml @00:25:32.60 delay 3 seconds @00:25:35.63 ATV.dial :air3 @00:25:36.18 delay 3 seconds @00:25:39.27 AS.seek 0.605ml @00:25:44.63 delay 3 seconds @00:25:47.66 ATV.dial :bleach @00:25:48.28 AS.seek 0.59ml @00:25:51.60 delay 3 seconds @00:25:54.63 ATV.dial ASV @00:25:55.65 ASV.dial :SPEdelivery @00:25:56.03 AS.seek 0ml @00:27:54.93 delay 1:00 @00:28:54.96 AFV.select :carrierSolution @00:28:55.06 delay 2 seconds @00:28:57.09 AS.reconfigure ASconfig @00:28:57.14 AS.seek 1ml @00:29:54.93 delay 1 second @00:29:55.95 AFV.select :holdingCoil @00:29:56.05 delay 1 second @00:29:57.08 ATV.dial ASV @00:29:57.09 ASV.dial :SPEdelivery @00:29:57.11 AS.reconfigure ASslow1 @00:29:57.15 AS.seek 0ml @00:33:18.53 delay 10 seconds @00:33:28.56 ATV.dial :air2 @00:33:29.39 AS.reconfigure ASslow0 @00:33:29.44 AS.seek 0.8ml @00:34:50.17 delay 3 seconds @00:34:53.20 ATV.dial :ASV @00:34:54.17 ASV.dial :SPEdelivery @00:34:54.19 AS.reconfigure ASslow1 @00:34:54.24 AS.seek 0.03ml @00:37:29.37 delay 20 seconds @00:37:49.40 ATV.dial :waste,via: :air3 @00:37:51.07 AS.reconfigure ASconfig @00:37:51.12 AS.seek :empty @00:37:53.21 SPE.coast @00:37:53.30 Removing Bleach from Mixing Coil @00:37:53.32 AFV.select :carrierSolution @00:37:53.42 delay 2 seconds @00:37:55.46 AS.seek 0.5ml @00:38:24.54 delay 1 second @00:38:25.56 AFV.select :holdingCoil @00:38:25.66 ATV.dial :ASV @00:38:27.43 ASV.dial :SPErecovery @00:38:28.07 AS.reconfigure ASslow1 @00:38:28.12 AS.seek 0.73ml @00:39:14.64 ASV.dial :wasteD @00:39:15.22 AS.reconfigure ASnorm @00:39:15.27 AS.seek 0ml @00:39:52.37 AFV.select :carrierSolution @00:39:52.48 delay 2 seconds @00:39:54.51 AS.reconfigure ASconfig @00:39:54.56 AS.seek 0.5ml @00:40:23.59 delay 1 second @00:40:24.61 AFV.select :holdingCoil @00:40:24.71 ATV.dial ASV @00:40:24.72 ASV.dial :SPErecovery @00:40:25.38 AS.reconfigure ASslow0 @00:40:25.43 AS.seek 0.3ml @00:40:45.83 ATV.dial :air2 @00:40:46.71 AS.reconfigure ASslow1 @00:40:46.76 AS.seek 0.32ml @00:40:51.10 ATV.dial ASV @00:40:52.14 AS.reconfigure ASslow0 @00:40:52.19 AS.seek 0.57ml @00:41:17.64 ASV.dial :wasteD @00:41:18.33 AS.reconfigure ASnorm @00:41:18.38 AS.seek 0ml @00:41:47.45 AFV.select :carrierSolution @00:41:47.56 delay 2 seconds @00:41:49.59 AS.reconfigure ASconfig @00:41:49.64 AS.seek 0.5ml @00:42:18.70 delay 1 second @00:42:19.72 AFV.select :holdingCoil @00:42:19.82 ATV.dial ASV @00:42:20.57 ASV.dial :SPErecovery @00:42:21.23 AS.reconfigure ASslow0 @00:42:21.28 AS.seek 0.3ml @00:42:41.72 ATV.dial :air2 @00:42:42.74 AS.reconfigure ASslow1 @00:42:42.79 AS.seek 0.32ml @00:42:47.12 ATV.dial ASV @00:42:48.15 AS.reconfigure ASslow0 @00:42:48.20 AS.seek 0.57ml @00:43:13.67 ASV.dial :wasteD @00:43:14.36 AS.reconfigure ASnorm @00:43:14.41 AS.seek 0ml @00:43:43.48 ATV.dial :air2 @00:43:44.43 AS.reconfigure ASslow0 @00:43:44.47 AS.seek 0.3ml @00:44:15.00 ATV.dial :ASV @00:44:15.97 ASV.dial :wasteD @00:44:15.99 AS.reconfigure ASconfig @00:44:16.04 AS.seek 0.05ml @00:44:30.72 ATV.dial :waste @00:44:32.54 AS.seek 0ml @00:44:35.73 ASV.dial :SPEdelivery @00:44:36.69 deconLysateINT @00:44:36.70 ATV.dial :air3 @00:44:37.38 AS.reconfigure ASslow1 @00:44:37.43 AS.seek 0.01ml @00:44:39.77 delay 2 seconds @00:44:41.79 ATV.dial :bleach @00:44:42.24 AS.seek 0.96ml @00:47:53.53 delay 3 seconds @00:47:56.56 ATV.dial :air3 @00:47:57.10 delay 3 seconds @00:48:00.19 AS.seek 0.985ml @00:48:05.47 delay 3 seconds @00:48:08.50 ATV.dial :bleach @00:48:09.10 AS.seek 0.97ml @00:48:12.42 delay 3 seconds @00:48:15.45 ATV.dial AIV @00:48:16.66 AIV.dial :coilToMFB @00:48:16.67 AWV.select :waste @00:48:16.69 AFV.select :carrierSolution @00:48:16.80 delay 2 seconds @00:48:18.83 AS.reconfigure ASconfig @00:48:18.88 AS.seek 1ml @00:48:20.92 delay 1 second @00:48:21.94 AFV.select :holdingCoil @00:48:22.04 delay 1 second @00:48:23.07 AS.reconfigure ASslow0 @00:48:23.12 AS.seek :empty @00:50:04.10 AFV.select :carrierSolution @00:50:04.21 delay 2 seconds @00:50:06.24 AS.reconfigure ASconfig @00:50:06.29 AS.seek 1ml @00:51:04.14 delay 1 second @00:51:05.16 AFV.select :holdingCoil @00:51:05.26 delay 1 second @00:51:06.29 AS.reconfigure ASslow0 @00:51:06.34 AS.seek :empty @00:52:47.33 AFV.select :carrierSolution @00:52:47.44 delay 2 seconds @00:52:49.47 AS.reconfigure ASconfig @00:52:49.52 AS.seek 1ml @00:53:47.38 delay 1 second @00:53:48.40 AFV.select :holdingCoil @00:53:48.50 delay 1 second @00:53:49.53 AS.reconfigure ASslow0 @00:53:49.58 AS.seek :empty @00:55:30.57 ATV.dial :air2 @00:55:31.23 AS.reconfigure ASslow1 @00:55:31.28 AS.seek 0.009ml @00:55:33.57 AS.reconfigure ASslow0 @00:55:33.62 AS.seek 1ml @00:57:13.55 ATV.dial AIV @00:57:14.30 AS.seek 0.02ml @00:58:53.18 ATV.dial :waste @00:58:54.77 AS.seek 0ml @00:58:57.08 AWV.select :air @00:58:57.26 Cleaning PCR @00:58:57.27 AFV.select :carrierSolution @00:58:57.38 delay 2 seconds @00:58:59.41 AS.reconfigure ASconfig @00:58:59.46 AS.seek 0.5ml @00:59:28.49 delay 1 second @00:59:29.51 AFV.select :holdingCoil @00:59:29.61 ASV.dial :waste1 @00:59:29.98 ATV.dial :air3 @00:59:30.66 AS.reconfigure ASslow1 @00:59:30.71 AS.seek 0.51ml @00:59:33.01 delay 2 seconds @00:59:35.79 ATV.dial :bleach @00:59:36.27 AS.seek 0.81ml @01:00:36.87 delay 3 seconds @01:00:39.90 ATV.dial :air3 @01:00:40.43 delay 3 seconds @01:00:43.52 AS.seek 0.835ml @01:00:48.88 delay 3 seconds @01:00:51.91 ATV.dial :bleach @01:00:52.52 AS.seek 0.82ml @01:00:55.82 delay 3 seconds @01:00:58.85 ARV.dial :waste @01:00:59.45 delay 3 seconds @01:01:02.48 ATV.dial :ARV @01:01:03.34 AS.reconfigure ASconfig @01:01:03.39 AS.seek 0.47ml @01:01:23.82 AFV.select :carrierSolution @01:01:23.93 delay 2 seconds @01:01:25.97 AS.seek 1ml @01:01:56.74 delay 1 second @01:01:57.76 AFV.select :holdingCoil @01:01:57.86 delay 1 second @01:01:58.89 AS.reconfigure ASfast @01:01:58.94 AS.seek 0ml @01:02:26.29 AFV.select :carrierSolution @01:02:26.40 delay 2 seconds @01:02:28.43 AS.reconfigure ASconfig @01:02:28.48 AS.seek 1ml @01:03:26.26 delay 1 second @01:03:27.28 AFV.select :holdingCoil @01:03:27.38 delay 1 second @01:03:28.41 AS.reconfigure ASfast @01:03:28.46 AS.seek 0ml @01:03:55.82 AFV.select :carrierSolution @01:03:55.93 delay 2 seconds @01:03:57.96 AS.reconfigure ASconfig @01:03:58.01 AS.seek 1ml @01:04:55.79 delay 1 second @01:04:56.81 AFV.select :holdingCoil @01:04:56.91 delay 1 second @01:04:57.94 AS.reconfigure ASfast @01:04:57.99 AS.seek 0ml @01:05:25.32 ARV.dial between carrier and air1 @01:05:25.81 ATV.dial :air3 @01:05:27.07 AS.reconfigure ASslow1 @01:05:27.12 AS.seek 0.01ml @01:05:29.45 delay 2 seconds @01:05:31.47 ATV.dial :bleach @01:05:32.08 AS.seek 0.81ml @01:08:13.22 delay 3 seconds @01:08:16.25 ATV.dial :air3 @01:08:16.79 delay 3 seconds @01:08:19.88 AS.seek 0.835ml @01:08:25.16 delay 3 seconds @01:08:28.19 ATV.dial :bleach @01:08:28.80 AS.seek 0.82ml @01:08:32.06 delay 3 seconds @01:08:35.09 ATV.dial PCR @01:08:35.48 AS.reconfigure ASconfig @01:08:35.53 AS.seek :empty @01:09:22.98 PCR.program :bleachProfile @01:09:23.04 PCR program should complete in < 2:45 @01:09:23.05 PCR.run 10:00 @01:12:49.11 PCR.off @01:12:49.14 AFV.select :carrierSolution @01:12:49.25 delay 2 seconds @01:12:51.29 AS.seek 1ml @01:13:49.11 delay 1 second @01:13:50.13 AFV.select :holdingCoil @01:13:50.23 delay 1 second @01:13:51.26 AS.reconfigure ASfast @01:13:51.31 AS.seek 0ml @01:14:18.67 delay 3 seconds @01:14:21.70 AFV.select :carrierSolution @01:14:21.80 delay 2 seconds @01:14:23.83 AS.reconfigure ASconfig @01:14:23.88 AS.seek 1ml @01:15:21.67 delay 1 second @01:15:22.69 AFV.select :holdingCoil @01:15:22.79 delay 1 second @01:15:23.82 AS.reconfigure ASfast @01:15:23.87 AS.seek 0ml @01:15:51.20 delay 3 seconds @01:15:54.23 AFV.select :carrierSolution @01:15:54.33 delay 2 seconds @01:15:56.36 AS.reconfigure ASconfig @01:15:56.42 AS.seek 1ml @01:16:54.21 delay 1 second @01:16:55.23 AFV.select :holdingCoil @01:16:56.09 delay 1 second @01:16:57.12 AS.reconfigure ASfast @01:16:57.17 AS.seek 0ml @01:17:24.52 delay 3 seconds @01:17:27.55 AFV.select :carrierSolution @01:17:27.65 delay 2 seconds @01:17:29.68 AS.reconfigure ASconfig @01:17:29.74 AS.seek 1ml @01:18:27.52 delay 1 second @01:18:28.54 AFV.select :holdingCoil @01:18:28.64 delay 1 second @01:18:29.67 AS.reconfigure ASfast @01:18:29.72 AS.seek 0ml @01:18:57.07 delay 3 seconds @01:19:00.10 AFV.select :carrierSolution @01:19:00.20 delay 2 seconds @01:19:02.23 AS.reconfigure ASconfig @01:19:02.29 AS.seek 1ml @01:20:00.11 delay 1 second @01:20:01.13 AFV.select :holdingCoil @01:20:01.23 delay 1 second @01:20:02.26 AS.reconfigure ASnorm @01:20:02.31 AS.seek 0ml @01:20:53.01 AFV.select :carrierSolution @01:20:53.12 delay 2 seconds @01:20:55.15 AS.reconfigure ASconfig @01:20:55.20 AS.seek 1ml @01:21:52.97 delay 1 second @01:21:53.99 AFV.select :holdingCoil @01:21:54.09 delay 1 second @01:21:55.12 ATV.dial :waste @01:21:56.31 AS.reconfigure ASnorm @01:21:56.36 AS.seek 0.5ml @01:22:21.93 ATV.dial :waste2 @01:22:23.17 AS.seek 0ml @01:22:48.68 AS.coast @01:22:48.72 SS.coast @01:22:48.76 CC.coast @01:22:48.80 CS.coast @01:22:48.84 PC.coast @01:22:48.88 PS.coast @01:22:48.92 Forearm.coast @01:22:48.97 Elbow.coast @01:22:49.36 Gate.power :core,:OFF @01:22:49.38 Gate.power :raw,:OFF @01:22:49.39 Shutdown @01:22:49.40 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @08:45:13.57 Session Begun @08:45:56.76 -> ESP.wakeup @08:45:56.87 Powering core bus @08:46:01.72 Gate.power :raw,:ON @08:46:02.43 SPE.coast @08:46:02.52 SPE.configure SPEconfig @08:46:02.65 AS.configure ASconfig @08:46:02.78 SS.configure SSconfig @08:46:02.92 CC.configure CCconfig @08:46:03.05 CS.configure CSconfig @08:46:03.18 PC.configure PCconfig @08:46:03.31 PS.configure PSconfig @08:46:03.47 Forearm.configure LooseForearm @08:46:03.60 Elbow.configure LooseElbow @08:46:03.75 SC.configure SCloose @08:46:03.88 SE.configure SEconfig @08:46:03.95 CH.coast @08:46:04.05 CH.configure CHconfig @08:46:04.11 PH.coast @08:46:04.21 PH.configure PHconfig @08:46:04.28 PCR.off @08:46:04.33 PCR.pidtemp= 50,0.1,0,300,25,25 @08:46:04.38 PCR.caltemp= 2.8814,0.0372,0 @08:46:04.43 All dwarf controllers running firmware version 3.75 @08:46:04.57 Valve.reset @08:46:04.76 PCR.off @08:46:04.81 PCR.pidtemp= 50,0.1,0,300,25,25 @08:46:04.86 PCR.caltemp= 2.8814,0.0372,0 @08:46:05.05 Intake.select :close @08:46:05.79 Exhaust.select :close @08:46:05.81 CTV.dial :air @08:46:05.93 PSV.dial :air @08:46:06.08 CSV.dial :waste1 @08:46:06.12 Forearm.reconfigure LooseForearm @08:46:06.25 Forearm.home.seek :retract @08:46:06.34 SSV.dial :spit,avoiding: :exhaust @08:46:06.49 PS.reconfigure PSconfig @08:46:06.56 PS.home.seek 0ml @08:46:07.01 Hand.close @08:46:07.05 Elbow.jog 1500 @08:46:07.24 CS.reconfigure CSfast @08:46:07.28 SS.reconfigure SSconfig @08:46:07.40 CS.home.seek 0ml @08:46:07.45 SS.home.seek 0ml @08:46:07.82 Elbow.reconfigure LooseElbow @08:46:07.89 Elbow.home.seek :clear @08:46:11.30 SE.reconfigure SEconfig @08:46:11.40 SE.home.seek :down @08:46:12.08 SC.reconfigure SCsloppy @08:46:12.15 SC.home.seek :initial @08:46:15.73 PS.coast @08:46:16.43 Forearm.seek :retract @08:46:16.54 Elbow.seek :hammer @08:46:17.82 Forearm.reconfigure TightForearm @08:46:17.91 Forearm.seek 5 + 350 counts @08:46:19.03 Forearm.seek :retract @08:46:20.28 CC.reconfigure CCconfig @08:46:20.29 PC.reconfigure PCconfig @08:46:20.42 CC.home.seek :open @08:46:20.48 PC.home.seek :open @08:46:31.01 -> MFB.ready! @08:46:31.10 Readying the MFB @08:46:31.13 Homing MFB Syringe @08:46:31.15 AFV.select :holdingCoil @08:46:31.35 ATV.dial :air2 @08:46:31.91 AS.reconfigure ASconfig @08:46:31.99 AS.home.seek :home @08:46:37.51 AFV.select :carrierSolution @08:46:37.64 delay 2 seconds @08:46:39.68 AS.seek 1ml @08:47:32.59 delay 1 second @08:47:33.61 AFV.select :holdingCoil @08:47:33.74 delay 1 second @08:47:34.77 ATV.dial :waste @08:47:35.81 AS.reconfigure ASnorm @08:47:35.90 AS.home.seek :empty @08:48:28.45 ATV.dial :waste @08:48:28.46 ARV.dial between carrier and air1 @08:48:28.59 ASV.dial :waste1 @08:49:04.54 -> ATV.ARV @08:49:04.56 ATV.dial ARV @08:49:41.92 -> ARV.air1 @08:49:41.94 ARV.dial :air1 @08:51:04.10 -> AS.pull 0.03 @08:51:04.20 AS.reconfigure ASconfig @08:51:04.28 AS.seek 0.029ml @08:51:15.62 -> ARV.PP1 @08:51:15.64 ARV.dial :PP1 @08:51:43.78 -> AS.pull 0.018, ASslow2 @08:51:43.89 AS.reconfigure ASslow2 @08:51:43.96 AS.seek 0.047ml @08:52:10.98 -> ARV.air2 @08:52:11.00 ARV.dial :air2 @08:52:23.15 -> AS.pull 0.03, ASslow1 @08:52:23.26 AS.reconfigure ASslow1 @08:52:23.33 AS.seek 0.077ml @08:53:00.24 -> ARV.PP2 @08:53:00.26 ARV.dial :PP2 @08:53:13.37 -> AS.pull 0.018, ASslow2 @08:53:13.48 AS.reconfigure ASslow2 @08:53:13.55 AS.seek 0.095ml @08:53:43.45 -> ARV.air2 @08:53:43.47 ARV.dial :air2 @08:53:52.41 -> AS.pull 0.03, ASslow1 @08:53:52.51 AS.reconfigure ASslow1 @08:53:52.59 AS.seek 0.125ml @08:54:25.37 -> ARV.mm1 @08:54:25.39 ARV.dial :mm1 @08:54:45.85 -> AS.pull 0.05, ASslow2 @08:54:46.56 AS.reconfigure ASslow2 @08:54:46.73 AS.seek 0.175ml @08:56:00.26 -> ARV.air2 @08:56:00.28 ARV.dial :air2 @08:56:12.10 -> AS.pull 0.03, ASslow1 @08:56:12.21 AS.reconfigure ASslow1 @08:56:12.28 AS.seek 0.205ml @08:56:32.58 -> pcrDecon @08:56:32.69 Cleaning PCR @08:56:32.70 AFV.select :carrierSolution @08:56:32.86 delay 2 seconds @08:56:34.89 AS.reconfigure ASconfig @08:56:34.97 AS.seek 0.5ml @08:56:52.20 delay 1 second @08:56:53.22 AFV.select :holdingCoil @08:56:53.35 ASV.dial :waste1 @08:56:53.37 ATV.dial :air3 @08:56:54.63 AS.reconfigure ASslow1 @08:56:54.70 AS.seek 0.51ml @08:56:56.99 delay 2 seconds @08:56:59.01 ATV.dial :bleach @08:56:59.63 AS.seek 0.81ml @08:58:00.21 delay 3 seconds @08:58:03.24 ATV.dial :air3 @08:58:03.80 delay 3 seconds @08:58:06.89 AS.seek 0.835ml @08:58:12.20 delay 3 seconds @08:58:15.23 ATV.dial :bleach @08:58:15.85 AS.seek 0.82ml @08:58:19.16 delay 3 seconds @08:58:22.19 ARV.dial :waste @08:58:23.63 delay 3 seconds @08:58:26.66 ATV.dial :ARV @08:58:27.56 AS.reconfigure ASconfig @08:58:27.63 AS.seek 0.47ml @08:58:48.07 AFV.select :carrierSolution @08:58:48.21 delay 2 seconds @08:58:50.25 AS.seek 1ml @08:59:21.03 delay 1 second @08:59:22.05 AFV.select :holdingCoil @08:59:22.18 delay 1 second @08:59:23.21 AS.reconfigure ASfast @08:59:23.29 AS.seek 0ml @08:59:50.64 AFV.select :carrierSolution @08:59:50.78 delay 2 seconds @08:59:52.81 AS.reconfigure ASconfig @08:59:52.89 AS.seek 1ml @09:00:50.71 delay 1 second @09:00:51.73 AFV.select :holdingCoil @09:00:51.86 delay 1 second @09:00:52.89 AS.reconfigure ASfast @09:00:52.96 AS.seek 0ml @09:01:20.35 AFV.select :carrierSolution @09:01:20.48 delay 2 seconds @09:01:22.51 AS.reconfigure ASconfig @09:01:22.59 AS.seek 1ml @09:02:20.40 delay 1 second @09:02:21.42 AFV.select :holdingCoil @09:02:21.55 delay 1 second @09:02:22.58 AS.reconfigure ASfast @09:02:22.66 AS.seek 0ml @09:02:50.04 ARV.dial between carrier and air1 @09:02:50.55 ATV.dial :air3 @09:02:51.83 AS.reconfigure ASslow1 @09:02:51.90 AS.seek 0.01ml @09:02:54.27 delay 2 seconds @09:02:56.29 ATV.dial :bleach @09:02:56.92 AS.seek 0.81ml @09:05:38.09 delay 3 seconds @09:05:41.12 ATV.dial :air3 @09:05:41.68 delay 3 seconds @09:05:44.77 AS.seek 0.835ml @09:05:50.11 delay 3 seconds @09:05:53.14 ATV.dial :bleach @09:05:53.76 AS.seek 0.82ml @09:05:57.12 delay 3 seconds @09:06:00.15 ATV.dial PCR @09:06:00.57 AS.reconfigure ASconfig @09:06:00.65 AS.seek :empty @09:06:48.13 PCR.program :bleachProfile @09:06:48.20 PCR program should complete in < 2:45 @09:06:48.22 PCR.run 10:00 @09:10:23.39 PCR.off @09:10:23.44 AFV.select :carrierSolution @09:10:23.57 delay 2 seconds @09:10:25.61 AS.seek 1ml @09:11:23.45 delay 1 second @09:11:24.47 AFV.select :holdingCoil @09:11:24.60 delay 1 second @09:11:25.63 AS.reconfigure ASfast @09:11:25.71 AS.seek 0ml @09:11:53.06 delay 3 seconds @09:11:56.09 AFV.select :carrierSolution @09:11:56.22 delay 2 seconds @09:11:58.25 AS.reconfigure ASconfig @09:11:58.33 AS.seek 1ml @09:12:56.15 delay 1 second @09:12:57.17 AFV.select :holdingCoil @09:12:57.30 delay 1 second @09:12:58.33 AS.reconfigure ASfast @09:12:58.40 AS.seek 0ml @09:13:25.78 delay 3 seconds @09:13:28.81 AFV.select :carrierSolution @09:13:28.94 delay 2 seconds @09:13:30.97 AS.reconfigure ASconfig @09:13:31.76 AS.seek 1ml @09:14:29.62 delay 1 second @09:14:30.64 AFV.select :holdingCoil @09:14:30.77 delay 1 second @09:14:31.80 AS.reconfigure ASfast @09:14:31.87 AS.seek 0ml @09:14:59.25 delay 3 seconds @09:15:02.28 AFV.select :carrierSolution @09:15:02.41 delay 2 seconds @09:15:04.44 AS.reconfigure ASconfig @09:15:04.52 AS.seek 1ml @09:16:02.34 delay 1 second @09:16:03.36 AFV.select :holdingCoil @09:16:03.49 delay 1 second @09:16:04.52 AS.reconfigure ASfast @09:16:04.59 AS.seek 0ml @09:16:31.97 delay 3 seconds @09:16:35.00 AFV.select :carrierSolution @09:16:35.13 delay 2 seconds @09:16:37.16 AS.reconfigure ASconfig @09:16:37.25 AS.seek 1ml @09:17:35.06 delay 1 second @09:17:36.08 AFV.select :holdingCoil @09:17:36.21 delay 1 second @09:17:37.24 AS.reconfigure ASnorm @09:17:37.32 AS.seek 0ml @09:19:32.56 -> ESP.shutdown @09:19:32.59 AS.coast @09:19:32.64 Asleep / Shutdown @09:19:32.72 SS.coast @09:19:32.79 CC.coast @09:19:32.85 CS.coast @09:19:32.92 PC.coast @09:19:32.98 Forearm.coast @09:19:33.05 Elbow.coast @09:19:33.11 SC.coast @09:19:33.18 SE.coast @09:19:33.59 Gate.power :core,:OFF @09:19:33.61 Gate.power :raw,:OFF @09:19:33.64 Shutdown @09:19:40.96 -> exit @09:19:40.98 Session Ended @10:00:00.05
Powering core bus @10:00:04.87 Gate.power :raw,:ON @10:00:05.53 SPE.coast @10:00:05.59 SPE.configure SPEconfig @10:00:05.68 AS.configure ASconfig @10:00:05.79 SS.configure SSconfig @10:00:05.89 CC.configure CCconfig @10:00:05.98 CS.configure CSconfig @10:00:06.08 PC.configure PCconfig @10:00:06.18 PS.configure PSconfig @10:00:06.30 Forearm.configure LooseForearm @10:00:06.40 Elbow.configure LooseElbow @10:00:06.52 SC.configure SCloose @10:00:06.62 SE.configure SEconfig @10:00:06.67 CH.coast @10:00:06.72 CH.configure CHconfig @10:00:06.76 PH.coast @10:00:06.82 PH.configure PHconfig @10:00:06.86 PCR.off @10:00:06.89 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:06.93 PCR.caltemp= 2.8814,0.0372,0 @10:00:06.96 All dwarf controllers running firmware version 3.75 @10:00:07.07 Valve.reset @10:00:07.24 PCR.off @10:00:07.27 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:07.30 PCR.caltemp= 2.8814,0.0372,0 @10:00:07.52 Intake.select :close @10:00:07.54 Exhaust.select :close @10:00:07.55 CTV.dial :air @10:00:07.64 PSV.dial :air @10:00:07.68 CSV.dial :waste1 @10:00:07.87 SSV.dial :spit,avoiding: :exhaust @10:00:07.90
Forearm.reconfigure LooseForearm @10:00:07.92 PS.reconfigure PSconfig @10:00:08.05 PS.home.seek 0ml @10:00:08.08
Forearm.home.seek :retract @10:00:08.26 CS.reconfigure CSfast @10:00:08.30 SS.reconfigure SSconfig @10:00:08.39 CS.home.seek 0ml @10:00:08.43 SS.home.seek 0ml @10:00:08.87 Hand.close @10:00:08.91
Elbow.jog 1500 @10:00:09.48 Elbow.reconfigure LooseElbow @10:00:09.52 Elbow.home.seek :clear @10:00:14.25 SE.reconfigure SEconfig @10:00:14.29 SE.home.seek :down @10:00:15.02 SC.reconfigure SCsloppy @10:00:15.07 SC.home.seek :initial @10:00:17.23 PS.coast @10:00:19.06
Forearm.seek :retract @10:00:19.14 Elbow.seek :hammer @10:00:21.00 Forearm.reconfigure TightForearm @10:00:21.08 Forearm.seek 5 + 350 counts @10:00:22.13 Forearm.seek :retract @10:00:23.32 PC.reconfigure PCconfig @10:00:23.34
CC.reconfigure CCconfig @10:00:23.44 PC.home.seek :open @10:00:23.46
CC.home.seek :open @10:00:26.99 GoodNews.email "At 10:00:26 began in tube 3: wcrphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:155\n{1 => 22, 2 => 21, 3 => 1}\nCan@10:00:26, 8.9C, 50% humidity, 14.3psia, 13.009V, 0.481A, 0.481A avg, 6.25W",Subject: "Started 20invasivedeployfinal phase wcrphase" @10:00:27.03
Phase [wcrphase] @10:00:27.05CEST27-May-20 ===> STARTED WCRSphase (phase #17) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @10:00:27.06 Starting initialPurge @10:00:27.08 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:00:27.15 Hand.open @10:00:28.08 Elbow.reconfigure TightElbow @10:00:28.14 Elbow.seek FlushPuck::Garage @10:00:30.21 Elbow.reconfigure LooseElbow @10:00:30.31 Forearm.reconfigure LooseForearm @10:00:30.40 Forearm.seek :garage @10:00:31.42 Hand.close @10:00:32.20 Forearm.seek :retract @10:00:33.25 Elbow.reconfigure TightElbow @10:00:33.29 Elbow.seek CC @10:00:36.35 Elbow.reconfigure LooseElbow @10:00:36.59 Forearm.reconfigure TightForearm @10:00:36.67 Forearm.seek CC @10:00:38.35 CC.seek :closed @10:00:42.81 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.15 @10:00:42.96 Hand.open @10:00:43.90 Forearm.reconfigure LooseForearm @10:00:43.99 Forearm.seek :retract @10:00:45.05 Flushing the Collection Valve::Manifold @10:00:45.34 CSV.dial CSR @10:00:46.96 Pulling 5ml of CSR.flush @10:00:46.98 CSR.select :flush @10:00:47.15 CS.reconfigure CSconfig @10:00:47.24 CS.seek 5ml @10:01:01.12 delay 5 seconds @10:01:06.16 CSR.select :air @10:01:06.41 CS.reconfigure CSfast @10:01:06.50 CS.seek 7.501ml @10:01:13.58 CSV.dial :waste @10:01:15.06 CS.reconfigure CSconfig @10:01:15.19 CS.seek 0ml @10:01:35.33 Purging external Intake @10:01:35.48 CSV.dial :flush @10:01:35.96 CS.reconfigure CSfast @10:01:36.01 CS.seek 10ml @10:02:00.87 delay 5 seconds @10:02:05.90 CSV.dial CBV @10:02:05.93
CBV.dial CSV @10:02:06.76 CTV.dial :intake @10:02:07.57 Intake.select :open @10:02:07.69 CS.seek 0ml @10:02:34.03 Intake.select :close @10:02:34.14 CTV.dial :air @10:02:34.98 Flushing upper manifold @10:02:35.11 CSV.dial :flush @10:02:35.93 CS.seek 5ml @10:02:48.75 delay 5 seconds @10:02:53.78 CSV.dial :air @10:02:55.00 CS.seek 7ml @10:03:00.84 CSV.dial CBV @10:03:00.86
CBV.dial CSV @10:03:02.00 CTV.dial :air @10:03:02.02 CS.seek 0ml @10:03:19.62 delay 7 seconds @10:03:26.66 CS.seek 3ml @10:03:34.87 CSV.dial :waste @10:03:35.37 CS.seek 0ml @10:03:43.59 CSV.dial :flush @10:03:44.09 CS.seek 3ml @10:03:52.15 delay 5 seconds @10:03:57.18 CSV.dial :air @10:03:57.74 CS.seek 5ml @10:04:03.53 CSV.dial :air @10:04:03.55 CS.seek 0ml @10:04:16.37 delay 7 seconds @10:04:23.41 CS.seek 3ml @10:04:31.65 CSV.dial :waste @10:04:32.46 CS.seek 0ml @10:04:40.62 Garaging FlushPuck in Collection Clamp @10:04:40.70 Elbow.reconfigure TightElbow @10:04:40.75 Elbow.seek CC @10:04:40.98 Elbow.reconfigure LooseElbow @10:04:41.08 Forearm.seek CC @10:04:42.11 Hand.close @10:04:42.83 CC.seek :open @10:04:47.19 Forearm.seek :retract @10:04:48.22 Elbow.reconfigure TightElbow @10:04:48.27 Elbow.seek :garage @10:04:51.47 Elbow.reconfigure LooseElbow @10:04:51.57 Forearm.reconfigure TightForearm @10:04:51.62 Forearm.seek :garage @10:04:52.77 Hand.open @10:04:53.53 Forearm.reconfigure LooseForearm @10:04:53.57 Forearm.seek :retract @10:04:54.61 Starting stand-alone bac WCR @10:04:54.62 bac wcr sampling at most 1500ml @10:04:54.65 BACfans.email "Started stand-alone bac WCR in tube 3 at 10:04:54CEST27-May-20\n{1 => 22, 2 => 21, 3 => 1}\nCan@10:00:26, 8.9C, 50% humidity, 14.3psia, 13.009V, 0.481A, 0.481A avg, 6.25W",Subject: "bac wcr sampling at most 1500ml" @10:04:54.68 Whole Cell Archive sampling 1500ml @10:04:54.72 Loading wcr puck from tube 3 into Collection Clamp @10:04:54.99 SC.reconfigure SCtight @10:04:55.04 SC.seek 3 @10:04:57.17 SC.reconfigure SCloose @10:04:57.53 Elbow.reconfigure TightElbow @10:04:57.69 Elbow.seek 3 @10:04:59.91 Forearm.reconfigure TightForearm @10:04:59.97 Forearm.seek 3 + 700 counts @10:05:01.03 SE.reconfigure SEpick @10:05:01.11 SE.seek beamBreak @10:05:04.24 SE.configure SEconfig @10:05:04.64 Elbow.seek 3 @10:05:05.12 Forearm.seek 3 + 300 counts @10:05:05.41 Forearm.reconfigure LooseForearm @10:05:05.57 Hand.close @10:05:06.48 SE.seek :down @10:05:07.03 Forearm.seek :retract @10:05:08.06 Elbow.seek CC @10:05:09.58 SE.coast @10:05:09.89 Elbow.reconfigure LooseElbow @10:05:10.19 Forearm.reconfigure TightForearm @10:05:10.29 Forearm.seek CC @10:05:12.05 CC.seek :closed @10:05:16.51 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.17 @10:05:16.55 Hand.open @10:05:17.49 Forearm.reconfigure LooseForearm @10:05:17.64 Forearm.seek :retract @10:05:18.79 SC.coast @10:05:18.88 collectSample 1500ml w/bubble point of 55psi @10:05:18.91 Priming 7ml @10:05:18.92 Intake.select :close @10:05:18.95 Exhaust.select :close @10:05:18.98 SS.hold @10:05:19.47 Calibrating pressure and temperature sensors @10:05:19.51 CTV.dial :air @10:05:19.54 SSV.dial :spit,avoiding: :exhaust @10:05:19.62 SS.reconfigure SSfast @10:05:19.73 SS.seek 5ml @10:05:22.71 SSV.dial CBV @10:05:22.79 CBV.dial SSV @10:05:24.30 delay 3.8 seconds @10:05:28.40 SSV.dial :spit @10:05:29.92 SS.seek 0ml @10:05:32.68 SSV.dial CBV @10:05:34.19 CTV.dial :intake @10:05:35.12 SSV.dial CBV @10:05:35.17 CBV.dial SSV @10:05:35.21 Allowing 40s for 7.0ml of remaining 7.0ml @10:05:35.23 Intake.select :open @10:05:37.53 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:05:37.57 SS.reconfigure SSinhale.with maxSpeed: 150 @10:05:37.66 SS.seek 7ml @10:05:43.58 Stroke completed -- waiting for pressure to equalize ... @10:05:57.74 Intake.select :close @10:05:57.99 SSV.dial :exhaust @10:05:58.88 Exhaust.select :open @10:05:59.05 SS.reconfigure SSexhale @10:05:59.15 SS.seek 0ml @10:06:03.11 Exhaust.select :close @10:06:03.27 Sampling 1500.0ml @10:06:03.30 Intake.select :close @10:06:03.32 Exhaust.select :close @10:06:03.35 SS.reconfigure SSconfig @10:06:03.43 SS.hold @10:06:03.88 CTV.dial :intake @10:06:03.93 SSV.dial CBV @10:06:03.98 CBV.dial SSV @10:06:04.90 Allowing 130s for 25.0ml of remaining 1500.0ml @10:06:04.94 Intake.select :open @10:06:07.20 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:06:07.24 SS.reconfigure SSinhale.with maxSpeed: 150 @10:06:07.34 SS.seek 25ml @10:06:11.04 Vacuum too high -- waiting up to 40s @10:06:13.40 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:06:13.42 SS.reconfigure SSinhale.with maxSpeed: 135 @10:06:13.47 SS.seek 25ml @10:06:16.40 Vacuum too high -- waiting up to 40s @10:06:18.97 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:06:18.99 SS.reconfigure SSinhale.with maxSpeed: 122 @10:06:19.04 SS.seek 25ml @10:06:21.92 Vacuum too high -- waiting up to 40s @10:06:24.50 SS.reconfigure SSinhale.with maxSpeed: 110 @10:06:24.55 SS.seek 25ml @10:06:27.18 Vacuum too high -- waiting up to 40s @10:06:29.48 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:06:29.50 SS.reconfigure SSinhale.with maxSpeed: 99 @10:06:29.55 SS.seek 25ml @10:06:31.83 Vacuum too high -- waiting up to 40s @10:06:34.20 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:06:34.22 SS.reconfigure SSinhale.with maxSpeed: 89 @10:06:34.27 SS.seek 25ml @10:06:36.96 Vacuum too high -- waiting up to 40s @10:06:39.11 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:06:39.13 SS.reconfigure SSinhale.with maxSpeed: 80 @10:06:39.18 SS.seek 25ml @10:06:41.55 Vacuum too high -- waiting up to 40s @10:06:43.62 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:06:43.64 SS.reconfigure SSinhale.with maxSpeed: 72 @10:06:43.69 SS.seek 25ml @10:06:46.29 Vacuum too high -- waiting up to 40s @10:06:48.39 SS.reconfigure SSinhale.with maxSpeed: 65 @10:06:48.44 SS.seek 25ml @10:06:51.12 Vacuum too high -- waiting up to 40s @10:06:53.19 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:06:53.21 SS.reconfigure SSinhale.with maxSpeed: 59 @10:06:53.26 SS.seek 25ml @10:06:56.99 Stroke completed -- waiting for pressure to equalize ... @10:07:02.74 Intake.select :close @10:07:02.90 SSV.dial :exhaust @10:07:03.74 Exhaust.select :open @10:07:03.86 SS.reconfigure SSexhale @10:07:03.91 SS.seek 0ml @10:07:13.06 Exhaust.select :close @10:07:13.18 SSV.dial CBV @10:07:13.20 CBV.dial SSV @10:07:14.05 Allowing 130s for 25.0ml of remaining 1475.0ml @10:07:14.06 Intake.select :open @10:07:16.24 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:07:16.26 SS.reconfigure SSinhale.with maxSpeed: 59 @10:07:16.31 SS.seek 25ml @10:07:20.93 Vacuum too high -- waiting up to 40s @10:07:23.00 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:07:23.02 SS.reconfigure SSinhale.with maxSpeed: 53 @10:07:23.07 SS.seek 25ml @10:07:26.40 Vacuum too high -- waiting up to 40s @10:07:28.73 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:07:28.75 SS.reconfigure SSinhale.with maxSpeed: 48 @10:07:28.80 SS.seek 25ml @10:07:32.65 Vacuum too high -- waiting up to 40s @10:07:34.97 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:07:34.99 SS.reconfigure SSinhale.with maxSpeed: 43 @10:07:35.04 SS.seek 25ml @10:07:39.40 Vacuum too high -- waiting up to 40s @10:07:41.93 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:07:41.95 SS.reconfigure SSinhale.with maxSpeed: 39 @10:07:42.00 SS.seek 25ml @10:07:47.08 Vacuum too high -- waiting up to 40s @10:07:49.49 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:07:49.51 SS.reconfigure SSinhale.with maxSpeed: 35 @10:07:49.56 SS.seek 25ml @10:07:54.37 Vacuum too high -- waiting up to 40s @10:07:56.94 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:07:56.96 SS.reconfigure SSinhale.with maxSpeed: 32 @10:07:57.01 SS.seek 25ml @10:08:03.77 Vacuum too high -- waiting up to 40s @10:08:06.59 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:08:06.61 SS.reconfigure SSinhale.with maxSpeed: 29 @10:08:06.65 SS.seek 25ml @10:08:14.05 Vacuum too high -- waiting up to 40s @10:08:17.12 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:08:17.14 SS.reconfigure SSinhale.with maxSpeed: 26 @10:08:17.19 SS.seek 25ml @10:08:25.65 Vacuum too high -- waiting up to 40s @10:08:28.98 Intake @31.5psia, vacuum from 20.0 to 24.0psi @10:08:29.00 SS.reconfigure SSinhale.with maxSpeed: 23 @10:08:29.05 SS.seek 25ml @10:08:33.60 Stroke completed -- waiting for pressure to equalize ... @10:08:42.45 Intake.select :close @10:08:42.61 SSV.dial :exhaust @10:08:43.45 Exhaust.select :open @10:08:43.57 SS.reconfigure SSexhale @10:08:43.62 SS.seek 0ml @10:08:52.97 Exhaust.select :close @10:08:53.09 SSV.dial CBV @10:08:53.11 CBV.dial SSV @10:08:53.99 Allowing 130s for 25.0ml of remaining 1450.0ml @10:08:54.00 Intake.select :open @10:08:56.17 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:08:56.19 SS.reconfigure SSinhale.with maxSpeed: 23 @10:08:56.24 SS.seek 25ml @10:09:11.48 Vacuum too high -- waiting up to 40s @10:09:14.57 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:09:14.59 SS.reconfigure SSinhale.with maxSpeed: 21 @10:09:14.63 SS.seek 25ml @10:09:27.04 Vacuum too high -- waiting up to 40s @10:09:30.83 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:09:30.85 SS.reconfigure SSinhale.with maxSpeed: 19 @10:09:30.89 SS.seek 25ml @10:09:47.55 Vacuum too high -- waiting up to 40s @10:09:51.67 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:09:51.69 SS.reconfigure SSinhale.with maxSpeed: 17 @10:09:51.73 SS.seek 25ml @10:10:11.80 Vacuum too high -- waiting up to 40s @10:10:15.94 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:10:15.96 SS.reconfigure SSinhale.with maxSpeed: 15 @10:10:16.01 SS.seek 25ml @10:10:46.90 Vacuum too high -- waiting up to 40s @10:10:51.95 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:10:51.97 SS.reconfigure SSinhale.with maxSpeed: 14 @10:10:52.02 SS.seek 25ml @10:10:57.27 Stroke completed -- waiting for pressure to equalize ... @10:11:04.01 SS.reconfigure SSconfig @10:11:04.06 SS.hold @10:11:04.19 Intake.select :close @10:11:04.35 ShallowSampler::Clogged in WCR -- progress too slow after 75.0ml @10:11:04.37 SSV.dial :exhaust @10:11:05.21 Exhaust.select :open @10:11:05.33 SS.reconfigure SSexhale @10:11:05.38 SS.seek 0ml @10:11:14.63 Exhaust.select :close @10:11:14.74 Sampled 75.0ml @10:11:14.76 Drying puck before fixing for 4:00 @10:11:14.77 CBV.dial :waste2b @10:11:15.62 delay 1:00 @10:12:15.65 CSV.dial :air @10:12:16.47 CS.seek 10ml @10:12:41.29 Pumping wcr puck from tube 3 up to 55psi... @10:12:41.30 CBV.dial SSV @10:12:42.71 CBV.dial between CSV and waste2b @10:12:43.78 CSV.dial between air and CTV @10:12:44.11 CS.seek 7.294ml @10:12:51.60 CTV.dial CSV,avoiding: :air @10:12:51.64 CSV.dial CTV @10:12:52.51 CS.reconfigure CSpressurize @10:12:52.57 CS.seek 0ml @10:13:10.78 CBV.dial :waste2b @10:13:11.15 CS.reconfigure CSconfig @10:13:11.20 CS.seek :empty @10:13:13.42 delay 1:00 @10:14:13.45 CTV.dial :air,avoiding: :intake @10:14:14.92 Fix #1 of 2 (with rnal) for 2:00 @10:14:14.94 CSV.dial CSR @10:14:15.48 Pulling 2ml of CSR.rnal @10:14:15.49 CSR.select :rnal @10:14:15.62 CS.seek 2ml @10:14:21.76 delay 5 seconds @10:14:26.79 CSR.select :air @10:14:26.92 CS.reconfigure CSfast @10:14:26.97 CS.seek 10ml @10:14:47.01 Pumping wcr puck from tube 3 up to 55psi... @10:14:47.02 CBV.dial SSV @10:14:48.41 CBV.dial between CSV and waste2b @10:14:49.49 CSV.dial between air and CTV @10:14:50.18 CS.seek 7.162ml @10:14:58.02 CTV.dial CSV,avoiding: :air @10:14:58.04 CSV.dial CTV @10:14:59.60 CS.reconfigure CSpressurize @10:14:59.65 CS.seek 0ml @10:15:17.19 delay 2:00 @10:17:17.22 CBV.dial :waste2b @10:17:17.59 CS.reconfigure CSconfig @10:17:17.64 CS.seek :empty @10:17:19.89 delay 1:00 @10:18:19.92 CTV.dial :air,avoiding: :intake @10:18:21.39 Fix #2 of 2 (with rnal) for 2:00 @10:18:21.41 CSV.dial CSR @10:18:21.95 Pulling 2ml of CSR.rnal @10:18:21.97 CSR.select :rnal @10:18:22.09 CS.seek 2ml @10:18:28.26 delay 5 seconds @10:18:33.29 CSR.select :air @10:18:33.42 CS.reconfigure CSfast @10:18:33.47 CS.seek 10ml @10:18:53.45 Pumping wcr puck from tube 3 up to 55psi... @10:18:53.47 CBV.dial SSV @10:18:54.86 CBV.dial between CSV and waste2b @10:18:55.93 CSV.dial between air and CTV @10:18:56.73 CS.seek 7.215ml @10:19:04.46 CTV.dial CSV,avoiding: :air @10:19:04.48 CSV.dial CTV @10:19:06.01 CS.reconfigure CSpressurize @10:19:06.06 CS.seek 0ml @10:19:24.38 delay 2:00 @10:21:24.42 CBV.dial :waste2b @10:21:24.77 CS.reconfigure CSconfig @10:21:24.82 CS.seek :empty @10:21:26.33 delay 1:00 @10:22:26.36 CTV.dial :air,avoiding: :intake @10:22:27.89 Storing wcr puck in Collection Clamp to tube 2 @10:22:28.07 SC.reconfigure SCtight @10:22:28.13 SC.seek 2 @10:22:30.33 SC.reconfigure SCloose @10:22:30.56 Elbow.reconfigure TightElbow @10:22:30.61 Elbow.seek 2 @10:22:32.62 Forearm.reconfigure TightForearm @10:22:32.67 Forearm.seek 2 + 700 counts @10:22:33.71 SE.reconfigure SEpick @10:22:33.79 SE.seek beamBreak @10:22:34.99 SE.configure SEconfig @10:22:35.15 Elbow.reconfigure LimpElbow @10:22:35.21 Forearm.reconfigure LooseForearm @10:22:35.26 Forearm.seek 2 @10:22:35.52 Elbow.hold @10:22:35.60 Elbow.reconfigure TightElbow @10:22:35.71 SE.seek 20.09 Pucks below Top Plate @10:22:36.37 Forearm.force -20 @10:22:36.42 Forearm.seek 2 + 339 counts @10:22:36.73 Forearm.reconfigure TightForearm @10:22:36.79 Forearm.seek 2 + 700 counts @10:22:37.79 SE.seek 21.09 Pucks below Top Plate @10:22:38.44 Forearm.reconfigure LooseForearm @10:22:38.49 Forearm.seek :retract @10:22:39.41 Elbow.seek CC @10:22:41.13 Elbow.reconfigure LooseElbow @10:22:41.24 Forearm.seek CC @10:22:42.32 Hand.close @10:22:43.12 CC.seek :open @10:22:47.62 Forearm.seek :retract @10:22:48.64 Elbow.reconfigure TightElbow @10:22:48.69 Elbow.seek 2 @10:22:50.68 Elbow.reconfigure LimpElbow @10:22:50.74 Forearm.seek 2 @10:22:51.77 Elbow.hold @10:22:51.85 Elbow.reconfigure TightElbow @10:22:51.90 Hand.open @10:22:52.74 Forearm.force -20 @10:22:52.79 Forearm.seek 2 + 344 counts @10:22:53.11 Forearm.reconfigure TightForearm @10:22:53.16 Forearm.seek 2 + 700 counts @10:22:53.54 SE.seek :down @10:22:54.32 SE.coast @10:22:54.69 Forearm.reconfigure LooseForearm @10:22:54.74 Forearm.seek :retract @10:22:55.66 SC.coast @10:22:55.70 Starting finalPurge @10:22:55.72 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:22:55.79 Hand.open @10:22:56.13 Elbow.seek FlushPuck::Garage @10:22:58.20 Elbow.reconfigure LooseElbow @10:22:58.31 Forearm.seek :garage @10:22:59.28 Hand.close @10:23:00.12 Forearm.seek :retract @10:23:01.10 Elbow.reconfigure TightElbow @10:23:01.15 Elbow.seek CC @10:23:04.08 Elbow.reconfigure LooseElbow @10:23:04.19 Forearm.reconfigure TightForearm @10:23:04.24 Forearm.seek CC @10:23:05.64 CC.seek :closed @10:23:09.89 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.10 @10:23:09.97 Hand.open @10:23:10.81 Forearm.reconfigure LooseForearm @10:23:10.86 Forearm.seek :retract @10:23:11.85 Flushing the Collection Valve::Manifold @10:23:11.99 CSV.dial CSR @10:23:12.53 Pulling 5ml of CSR.flush @10:23:12.54 CSR.select :flush @10:23:12.67 CS.seek 5ml @10:23:26.52 delay 5 seconds @10:23:31.55 CSR.select :air @10:23:31.71 CS.reconfigure CSfast @10:23:31.77 CS.seek 7.5ml @10:23:38.77 CSV.dial :waste @10:23:40.29 CS.reconfigure CSconfig @10:23:40.34 CS.seek 0ml @10:24:00.52 Flushing lower manifold's waste1 port @10:24:00.66 CSV.dial :flush @10:24:01.15 CS.reconfigure CSfast @10:24:01.20 CS.seek 5ml @10:24:14.05 delay 5 seconds @10:24:19.08 CSV.dial :air @10:24:19.64 CS.seek 7ml @10:24:25.46 CSV.dial CTV @10:24:25.49
CTV.dial CSV @10:24:27.02 CBV.dial :waste1 @10:24:27.84 CS.seek 0ml @10:24:45.40 Flushing lower manifold's waste2b port @10:24:45.54 CSV.dial :flush @10:24:46.36 CS.seek 5ml @10:24:59.21 delay 5 seconds @10:25:04.23 CSV.dial :air @10:25:04.80 CS.seek 7ml @10:25:10.59 CSV.dial CTV @10:25:10.63
CTV.dial CSV @10:25:11.12 CBV.dial :waste2b @10:25:11.97 CS.seek 0ml @10:25:29.55 Flushing upper manifold @10:25:29.68 CSV.dial :flush @10:25:30.50 CS.seek 5ml @10:25:43.37 delay 5 seconds @10:25:48.40 CSV.dial :air @10:25:48.97 CS.seek 7ml @10:25:54.71 CSV.dial CBV @10:25:54.75
CBV.dial CSV @10:25:55.93 CTV.dial :air @10:25:57.38 CS.seek 0ml @10:26:14.95 delay 7 seconds @10:26:21.99 CS.seek 3ml @10:26:30.09 CSV.dial :waste @10:26:30.60 CS.seek 0ml @10:26:39.18 CSV.dial :flush @10:26:39.69 CS.seek 3ml @10:26:47.77 delay 5 seconds @10:26:52.80 CSV.dial :air @10:26:53.35 CS.seek 5ml @10:26:59.15 CSV.dial :air @10:26:59.17 CS.seek 0ml @10:27:12.03 delay 7 seconds @10:27:19.07 CS.seek 3ml @10:27:27.31 CSV.dial :waste @10:27:28.13 CS.seek 0ml @10:27:36.24 Disinfecting external Intake with mfbkill @10:27:36.97 CSV.dial CSR @10:27:38.54 Pulling 8ml of CSR.mfbkill @10:27:38.55 CSR.select :mfbkill @10:27:38.68 CS.seek 8ml @10:27:59.21 delay 5 seconds @10:28:04.24 CSR.select :air @10:28:04.37 CS.seek 10ml @10:28:10.25 CSV.dial CBV @10:28:10.29
CBV.dial CSV @10:28:11.42 CTV.dial :intake @10:28:12.26 Intake.select :open @10:28:12.38 CS.seek 2ml @10:28:33.50 Intake.select :close @10:28:33.61 CTV.dial :air @10:28:34.43 CS.reconfigure CSconfig @10:28:34.48 CS.seek 0ml @10:28:40.53 Flushing lower manifold's waste1 port @10:28:40.54 CSV.dial CSR @10:28:41.73 Pulling 4ml of CSR.mfbkill @10:28:41.74 CSR.select :mfbkill @10:28:41.87 CS.seek 4ml @10:28:53.06 delay 5 seconds @10:28:58.09 CSR.select :air @10:28:58.21 CS.reconfigure CSfast @10:28:58.25 CS.seek 10ml @10:29:13.43 CSV.dial CTV @10:29:13.46
CTV.dial CSV @10:29:15.03 CBV.dial :waste1 @10:29:15.55 CS.seek 0ml @10:29:40.40 Flushing lower manifold's waste2b port @10:29:40.41 CSV.dial CSR @10:29:40.96 Pulling 4ml of CSR.mfbkill @10:29:40.97 CSR.select :mfbkill @10:29:41.10 CS.reconfigure CSconfig @10:29:41.15 CS.seek 4ml @10:29:52.31 delay 5 seconds @10:29:57.34 CSR.select :air @10:29:57.47 CS.reconfigure CSfast @10:29:57.51 CS.seek 10ml @10:30:12.66 CSV.dial CTV @10:30:12.68
CTV.dial CSV @10:30:13.20 CBV.dial :waste2b @10:30:14.05 CS.seek 0ml @10:30:38.78 Killing upper manifold @10:30:38.79 CSV.dial CSR @10:30:39.34 Pulling 4ml of CSR.mfbkill @10:30:39.35 CSR.select :mfbkill @10:30:39.47 CS.reconfigure CSconfig @10:30:39.52 CS.seek 4ml @10:30:50.72 delay 5 seconds @10:30:55.75 CSR.select :air @10:30:55.88 CS.reconfigure CSfast @10:30:55.92 CS.seek 10ml @10:31:11.09 CSV.dial CBV @10:31:11.12
CBV.dial CSV @10:31:12.39 CTV.dial :air @10:31:13.84 CS.seek 0ml @10:31:38.50 delay 7 seconds @10:31:45.54 CS.seek 3ml @10:31:53.66 CSV.dial :waste @10:31:54.17 CS.seek 0ml @10:32:02.31 Flushing upper manifold @10:32:02.44 CSV.dial :flush @10:32:02.93 CS.seek 5ml @10:32:15.81 delay 5 seconds @10:32:20.84 CSV.dial :air @10:32:21.41 CS.seek 7ml @10:32:27.25 CSV.dial CBV @10:32:27.29
CBV.dial CSV @10:32:28.41 CTV.dial :air @10:32:28.43 CS.seek 0ml @10:32:46.16 delay 7 seconds @10:32:53.20 CS.seek 3ml @10:33:01.41 CSV.dial :waste @10:33:01.92 CS.seek 0ml @10:33:10.09 CSV.dial :flush @10:33:10.57 CS.seek 3ml @10:33:18.75 delay 5 seconds @10:33:23.78 CSV.dial :air @10:33:25.01 CS.seek 5ml @10:33:30.75 CSV.dial :air @10:33:30.77 CS.seek 0ml @10:33:43.53 delay 7 seconds @10:33:50.57 CS.seek 3ml @10:33:58.88 CSV.dial :waste @10:33:59.70 CS.seek 0ml @10:34:07.81 Flushing lower manifold's waste1 port @10:34:07.95 CSV.dial :flush @10:34:08.45 CS.seek 5ml @10:34:21.19 delay 5 seconds @10:34:26.22 CSV.dial :air @10:34:26.78 CS.seek 7ml @10:34:32.60 CSV.dial CTV @10:34:32.63
CTV.dial CSV @10:34:34.16 CBV.dial :waste1 @10:34:34.67 CS.seek 0ml @10:34:52.22 Flushing lower manifold's waste2b port @10:34:52.36 CSV.dial :flush @10:34:53.17 CS.seek 5ml @10:35:06.00 delay 5 seconds @10:35:11.03 CSV.dial :air @10:35:11.60 CS.seek 7ml @10:35:17.38 CSV.dial CTV @10:35:17.41
CTV.dial CSV @10:35:17.89 CBV.dial :waste2b @10:35:18.73 CS.seek 0ml @10:35:36.19 Disinfecting external Intake with kill @10:35:36.32 CSV.dial CSR @10:35:36.86 Pulling 8ml of CSR.kill @10:35:36.87 CSR.select :kill @10:35:37.00 CS.seek 8ml @10:35:57.10 delay 5 seconds @10:36:02.13 CSR.select :air @10:36:02.26 CS.seek 10ml @10:36:08.13 CSV.dial CBV @10:36:08.16
CBV.dial CSV @10:36:09.33 CTV.dial :intake @10:36:10.14 Intake.select :open @10:36:10.26 CS.seek 2ml @10:36:31.41 Intake.select :close @10:36:31.52 CTV.dial :air @10:36:32.33 CS.reconfigure CSconfig @10:36:32.38 CS.seek 0ml @10:36:38.72 CSV.dial CSR @10:36:39.89 Pulling 4.999ml of CSR.flush @10:36:39.90 CSR.select :flush @10:36:40.02 CS.seek 5ml @10:36:53.79 delay 5 seconds @10:36:58.82 CSR.select :air @10:36:58.94 CS.seek 10ml @10:37:12.69 CSV.dial CBV @10:37:13.80 CS.seek 0ml @10:37:40.32 Garaging FlushPuck in Collection Clamp @10:37:40.40 Elbow.reconfigure TightElbow @10:37:40.45 Elbow.seek CC @10:37:40.67 Elbow.reconfigure LooseElbow @10:37:40.78 Forearm.seek CC @10:37:41.76 Hand.close @10:37:42.51 CC.seek :open @10:37:46.89 Forearm.seek :retract @10:37:47.87 Elbow.reconfigure TightElbow @10:37:47.92 Elbow.seek :garage @10:37:51.12 Elbow.reconfigure LooseElbow @10:37:51.22 Forearm.reconfigure TightForearm @10:37:51.27 Forearm.seek :garage @10:37:52.41 Hand.open @10:37:53.99 Forearm.reconfigure LooseForearm @10:37:54.08 Forearm.seek :retract @10:37:55.09 Starting initialPurge @10:37:55.10 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:37:55.18 Hand.open @10:37:55.46 Elbow.reconfigure TightElbow @10:37:55.51 Elbow.seek FlushPuck::Garage @10:37:55.63 Elbow.reconfigure LooseElbow @10:37:55.73 Forearm.seek :garage @10:37:56.75 Hand.close @10:37:57.53 Forearm.seek :retract @10:37:58.56 Elbow.reconfigure TightElbow @10:37:58.61 Elbow.seek CC @10:38:01.50 Elbow.reconfigure LooseElbow @10:38:01.61 Forearm.reconfigure TightForearm @10:38:01.65 Forearm.seek CC @10:38:03.00 CC.seek :closed @10:38:07.24 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.08 @10:38:07.32 Hand.open @10:38:08.08 Forearm.reconfigure LooseForearm @10:38:08.13 Forearm.seek :retract @10:38:09.11 Flushing the Collection Valve::Manifold @10:38:09.24 CSV.dial CSR @10:38:10.41 Pulling 5ml of CSR.flush @10:38:10.42 CSR.select :flush @10:38:10.54 CS.seek 5ml @10:38:24.33 delay 5 seconds @10:38:29.36 CSR.select :air @10:38:29.53 CS.reconfigure CSfast @10:38:29.58 CS.seek 7.5ml @10:38:36.48 CSV.dial :waste @10:38:37.91 CS.reconfigure CSconfig @10:38:37.96 CS.seek 0ml @10:38:58.10 Purging external Intake @10:38:58.23 CSV.dial :flush @10:38:58.73 CS.reconfigure CSfast @10:38:58.78 CS.seek 10ml @10:39:23.32 delay 5 seconds @10:39:28.35 CSV.dial CBV @10:39:28.39
CBV.dial CSV @10:39:29.34 CTV.dial :intake @10:39:30.17 Intake.select :open @10:39:30.29 CS.seek 0ml @10:39:56.42 Intake.select :close @10:39:56.53 CTV.dial :air @10:39:57.35 Flushing upper manifold @10:39:57.48 CSV.dial :flush @10:39:58.28 CS.seek 5ml @10:40:11.01 delay 5 seconds @10:40:16.04 CSV.dial :air @10:40:16.59 CS.seek 7ml @10:40:22.32 CSV.dial CBV @10:40:22.34
CBV.dial CSV @10:40:23.48 CTV.dial :air @10:40:23.50 CS.seek 0ml @10:40:41.01 delay 7 seconds @10:40:48.05 CS.seek 3ml @10:40:56.23 CSV.dial :waste @10:40:56.72 CS.seek 0ml @10:41:04.85 CSV.dial :flush @10:41:05.33 CS.seek 3ml @10:41:13.64 delay 5 seconds @10:41:18.66 CSV.dial :air @10:41:19.91 CS.seek 4.999ml @10:41:25.64 CSV.dial :air @10:41:25.66 CS.seek 0ml @10:41:38.54 delay 7 seconds @10:41:45.58 CS.seek 3ml @10:41:53.67 CSV.dial :waste @10:41:54.49 CS.seek 0ml @10:42:02.67 Garaging FlushPuck in Collection Clamp @10:42:02.75 Elbow.reconfigure TightElbow @10:42:02.80 Elbow.seek CC @10:42:03.02 Elbow.reconfigure LooseElbow @10:42:03.13 Forearm.seek CC @10:42:04.13 Hand.close @10:42:04.90 CC.seek :open @10:42:09.27 Forearm.seek :retract @10:42:10.26 Elbow.reconfigure TightElbow @10:42:10.31 Elbow.seek :garage @10:42:13.49 Elbow.reconfigure LooseElbow @10:42:13.59 Forearm.reconfigure TightForearm @10:42:13.64 Forearm.seek :garage @10:42:14.76 Hand.open @10:42:15.59 Forearm.reconfigure LooseForearm @10:42:15.64 Forearm.seek :retract @10:42:16.68 Completed [wcrphase] begun 10:00:26 @10:42:16.69 Preparing to sleep until 16:00 27-may-20 @10:42:16.76 SS.coast @10:42:16.78
Asleep til 16:00 27-may-20 @10:42:16.85 Continuing at 16:00 @10:42:16.86 CC.coast @10:42:16.95 CS.coast @10:42:17.01 GoodNews.email "Then, in tube 4: wcrphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:159\n{1 => 22, 2 => 22, 3 => 0}\nCan@10:42:16, 9.4C, 50% humidity, 14.4psia, 12.974V, 0.468A, 0.468A avg, 6.07W",Subject: "Asleep until 16:00" @10:42:17.04 PC.coast @10:42:17.11 Forearm.coast @10:42:17.15 Elbow.coast @10:42:17.55 Gate.power :core,:OFF @10:42:17.56 Gate.power :raw,:OFF @10:42:17.57 Shutdown @16:00:00.06
Powering core bus @16:00:04.75 Gate.power :raw,:ON @16:00:05.42 SPE.coast @16:00:05.49 SPE.configure SPEconfig @16:00:05.58 AS.configure ASconfig @16:00:05.68 SS.configure SSconfig @16:00:05.78 CC.configure CCconfig @16:00:05.88 CS.configure CSconfig @16:00:05.98 PC.configure PCconfig @16:00:06.07 PS.configure PSconfig @16:00:06.19 Forearm.configure LooseForearm @16:00:06.29 Elbow.configure LooseElbow @16:00:06.41 SC.configure SCloose @16:00:06.50 SE.configure SEconfig @16:00:06.55 CH.coast @16:00:06.60 CH.configure CHconfig @16:00:07.31 PH.coast @16:00:07.37 PH.configure PHconfig @16:00:07.41 PCR.off @16:00:07.44 PCR.pidtemp= 50,0.1,0,300,25,25 @16:00:07.48 PCR.caltemp= 2.8814,0.0372,0 @16:00:07.51 All dwarf controllers running firmware version 3.75 @16:00:07.62 Valve.reset @16:00:07.79 PCR.off @16:00:07.82 PCR.pidtemp= 50,0.1,0,300,25,25 @16:00:07.85 PCR.caltemp= 2.8814,0.0372,0 @16:00:08.00 Intake.select :close @16:00:08.06 Exhaust.select :close @16:00:08.10 CTV.dial :air @16:00:08.21 PSV.dial :air @16:00:08.26 CSV.dial :waste1 @16:00:08.42 SSV.dial :spit,avoiding: :exhaust @16:00:08.46 PS.reconfigure PSconfig @16:00:08.49
Forearm.reconfigure LooseForearm @16:00:08.63 PS.home.seek 0ml @16:00:08.65
Forearm.home.seek :retract @16:00:09.43 Hand.close @16:00:09.46
Elbow.jog 1500 @16:00:09.60 CS.reconfigure CSfast @16:00:09.64 SS.reconfigure SSconfig @16:00:09.72 CS.home.seek 0ml @16:00:09.76 SS.home.seek 0ml @16:00:10.07
Elbow.reconfigure LooseElbow @16:00:10.13 Elbow.home.seek :clear @16:00:13.60 SE.reconfigure SEconfig @16:00:13.65 SE.home.seek :down @16:00:14.28 SC.reconfigure SCsloppy @16:00:14.33 SC.home.seek :initial @16:00:17.71 PS.coast @16:00:18.53
Forearm.seek :retract @16:00:18.61 Elbow.seek :hammer @16:00:19.82 Forearm.reconfigure TightForearm @16:00:19.87 Forearm.seek 5 + 350 counts @16:00:20.95 Forearm.seek :retract @16:00:22.14 PC.reconfigure PCconfig @16:00:22.15
CC.reconfigure CCconfig @16:00:22.26 CC.home.seek :open @16:00:22.28 PC.home.seek :open @16:00:25.88
Phase [wcrphase] @16:00:25.89 GoodNews.email "At 16:00:25 began in tube 4: wcrphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:159\n{1 => 22, 2 => 22, 3 => 0}\nCan@16:00:25, 9.2C, 50% humidity, 14.4psia, 13.028V, 0.525A, 0.525A avg, 6.84W",Subject: "Started 20invasivedeployfinal phase wcrphase" @16:00:25.91CEST27-May-20
===> STARTED WCRSphase (phase #18) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @16:00:25.93 Starting initialPurge @16:00:25.97 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:00:26.04 Hand.open @16:00:26.84 Elbow.reconfigure TightElbow @16:00:26.89 Elbow.seek FlushPuck::Garage @16:00:28.89 Elbow.reconfigure LooseElbow @16:00:28.99 Forearm.reconfigure LooseForearm @16:00:29.04 Forearm.seek :garage @16:00:30.03 Hand.close @16:00:30.84 Forearm.seek :retract @16:00:31.85 Elbow.reconfigure TightElbow @16:00:31.90 Elbow.seek CC @16:00:34.92 Elbow.reconfigure LooseElbow @16:00:35.13 Forearm.reconfigure TightForearm @16:00:35.25 Forearm.seek CC @16:00:36.98 CC.seek :closed @16:00:41.52 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.27 @16:00:41.72 Hand.open @16:00:42.77 Forearm.reconfigure LooseForearm @16:00:42.86 Forearm.seek :retract @16:00:43.93 Flushing the Collection Valve::Manifold @16:00:44.21 CSV.dial CSR @16:00:45.81 Pulling 5ml of CSR.flush @16:00:45.83 CSR.select :flush @16:00:46.05 CS.reconfigure CSconfig @16:00:46.15 CS.seek 5ml @16:00:59.97 delay 5 seconds @16:01:05.00 CSR.select :air @16:01:05.26 CS.reconfigure CSfast @16:01:05.40 CS.seek 7.5ml @16:01:12.34 CSV.dial :waste @16:01:13.85 CS.reconfigure CSconfig @16:01:13.95 CS.seek 0ml @16:01:34.13 Purging external Intake @16:01:34.26 CSV.dial :flush @16:01:34.76 CS.reconfigure CSfast @16:01:34.81 CS.seek 10ml @16:01:59.44 delay 5 seconds @16:02:04.47 CSV.dial CBV @16:02:04.51
CBV.dial CSV @16:02:05.33 CTV.dial :intake @16:02:06.15 Intake.select :open @16:02:06.27 CS.seek 0ml @16:02:32.57 Intake.select :close @16:02:32.68 CTV.dial :air @16:02:33.51 Flushing upper manifold @16:02:33.64 CSV.dial :flush @16:02:34.44 CS.seek 5ml @16:02:47.29 delay 5 seconds @16:02:52.32 CSV.dial :air @16:02:53.55 CS.seek 7ml @16:02:59.26 CSV.dial CBV @16:02:59.28
CBV.dial CSV @16:03:00.41 CTV.dial :air @16:03:00.43 CS.seek 0ml @16:03:18.01 delay 7 seconds @16:03:25.05 CS.seek 3ml @16:03:33.13 CSV.dial :waste @16:03:33.63 CS.seek 0ml @16:03:42.06 CSV.dial :flush @16:03:42.55 CS.seek 3ml @16:03:50.76 delay 5 seconds @16:03:55.79 CSV.dial :air @16:03:56.45 CS.seek 5ml @16:04:02.20 CSV.dial :air @16:04:02.22 CS.seek 0ml @16:04:15.01 delay 7 seconds @16:04:22.05 CS.seek 3ml @16:04:30.26 CSV.dial :waste @16:04:31.05 CS.seek 0ml @16:04:39.41 Garaging FlushPuck in Collection Clamp @16:04:39.49 Elbow.reconfigure TightElbow @16:04:39.54 Elbow.seek CC @16:04:39.76 Elbow.reconfigure LooseElbow @16:04:39.87 Forearm.seek CC @16:04:40.86 Hand.close @16:04:41.59 CC.seek :open @16:04:45.95 Forearm.seek :retract @16:04:46.97 Elbow.reconfigure TightElbow @16:04:47.02 Elbow.seek :garage @16:04:50.18 Elbow.reconfigure LooseElbow @16:04:50.28 Forearm.reconfigure TightForearm @16:04:50.33 Forearm.seek :garage @16:04:51.48 Hand.open @16:04:52.29 Forearm.reconfigure LooseForearm @16:04:52.33 Forearm.seek :retract @16:04:53.37 Starting stand-alone bac WCR @16:04:53.38 bac wcr sampling at most 1500ml @16:04:53.41 Whole Cell Archive sampling 1500ml @16:04:53.43 BACfans.email "Started stand-alone bac WCR in tube 4 at 16:04:53CEST27-May-20\n{1 => 22, 2 => 22, 3 => 0}\nCan@16:00:25, 9.2C, 50% humidity, 14.4psia, 13.028V, 0.525A, 0.525A avg, 6.84W",Subject: "bac wcr sampling at most 1500ml" @16:04:53.45 Loading wcr puck from tube 4 into Collection Clamp @16:04:53.76 SC.reconfigure SCtight @16:04:53.81 SC.seek 4 @16:04:57.52 SC.reconfigure SCloose @16:04:57.79 Elbow.reconfigure TightElbow @16:04:57.85 Elbow.seek 4 @16:04:59.95 Forearm.reconfigure TightForearm @16:05:00.00 Forearm.seek 4 + 700 counts @16:05:01.12 SE.reconfigure SEpick @16:05:01.26 SE.seek beamBreak @16:05:03.88 SE.configure SEconfig @16:05:04.32 Elbow.seek 4 @16:05:04.87 Forearm.seek 4 + 300 counts @16:05:05.19 Forearm.reconfigure LooseForearm @16:05:05.29 Hand.close @16:05:06.04 SE.seek :down @16:05:06.57 Forearm.seek :retract @16:05:06.98 SE.coast @16:05:07.56 Elbow.seek CC @16:05:09.41 Elbow.reconfigure LooseElbow @16:05:09.70 Forearm.reconfigure TightForearm @16:05:09.82 Forearm.seek CC @16:05:11.54 CC.seek :closed @16:05:16.00 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.22 @16:05:16.03 Hand.open @16:05:16.19 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[61:27->20] @16:05:16.90 Forearm.reconfigure LooseForearm @16:05:17.00 Forearm.seek :retract @16:05:18.10 SC.coast @16:05:18.19 collectSample 1500ml w/bubble point of 55psi @16:05:18.22 Priming 7ml @16:05:18.24 Intake.select :close @16:05:18.26 Exhaust.select :close @16:05:18.29 SS.hold @16:05:18.77 Calibrating pressure and temperature sensors @16:05:18.85 CTV.dial :air @16:05:18.87 SSV.dial :spit,avoiding: :exhaust @16:05:18.92 SS.reconfigure SSfast @16:05:19.02 SS.seek 5ml @16:05:21.97 SSV.dial CBV @16:05:22.04 CBV.dial SSV @16:05:23.68 delay 3.8 seconds @16:05:27.79 SSV.dial :spit @16:05:29.30 SS.seek 0ml @16:05:32.25 SSV.dial CBV @16:05:33.73 CTV.dial :intake @16:05:34.65 SSV.dial CBV @16:05:34.70 CBV.dial SSV @16:05:34.75 Allowing 40s for 7.0ml of remaining 7.0ml @16:05:34.77 Intake.select :open @16:05:37.07 Intake @31.6psia, vacuum from 20.0 to 24.0psi @16:05:37.12 SS.reconfigure SSinhale.with maxSpeed: 150 @16:05:37.21 SS.seek 7ml @16:05:41.40 Vacuum too high -- waiting up to 40s @16:05:46.12 Intake @31.4psia, vacuum from 20.0 to 24.0psi @16:05:46.16 SS.reconfigure SSinhale.with maxSpeed: 135 @16:05:46.27 SS.seek 7ml @16:05:49.51 Stroke completed -- waiting for pressure to equalize ... @16:05:54.28 Intake.select :close @16:05:54.55 SSV.dial :exhaust @16:05:55.44 Exhaust.select :open @16:05:55.61 SS.reconfigure SSexhale @16:05:55.71 SS.seek 0ml @16:05:59.76 Exhaust.select :close @16:05:59.93 Sampling 1500.0ml @16:05:59.96 Intake.select :close @16:05:59.98 Exhaust.select :close @16:06:00.01 SS.reconfigure SSconfig @16:06:00.10 SS.hold @16:06:00.54 CTV.dial :intake @16:06:00.59 SSV.dial CBV @16:06:00.66 CBV.dial SSV @16:06:01.55 Allowing 130s for 25.0ml of remaining 1500.0ml @16:06:01.58 Intake.select :open @16:06:03.85 Intake @32.0psia, vacuum from 20.0 to 24.0psi @16:06:03.89 SS.reconfigure SSinhale.with maxSpeed: 135 @16:06:04.00 SS.seek 25ml @16:06:07.76 Vacuum too high -- waiting up to 40s @16:06:10.10 Intake @31.3psia, vacuum from 20.0 to 24.0psi @16:06:10.12 SS.reconfigure SSinhale.with maxSpeed: 122 @16:06:10.17 SS.seek 25ml @16:06:12.98 Vacuum too high -- waiting up to 40s @16:06:15.56 Intake @31.3psia, vacuum from 20.0 to 24.0psi @16:06:15.58 SS.reconfigure SSinhale.with maxSpeed: 110 @16:06:15.63 SS.seek 25ml @16:06:18.41 Vacuum too high -- waiting up to 40s @16:06:21.23 Intake @31.4psia, vacuum from 20.0 to 24.0psi @16:06:21.25 SS.reconfigure SSinhale.with maxSpeed: 99 @16:06:22.00 SS.seek 25ml @16:06:25.34 Vacuum too high -- waiting up to 40s @16:06:27.96 SS.reconfigure SSinhale.with maxSpeed: 89 @16:06:28.02 SS.seek 25ml @16:06:30.81 Vacuum too high -- waiting up to 40s @16:06:33.40 Intake @31.5psia, vacuum from 20.0 to 24.0psi @16:06:33.42 SS.reconfigure SSinhale.with maxSpeed: 80 @16:06:33.47 SS.seek 25ml @16:06:36.31 Vacuum too high -- waiting up to 40s @16:06:38.90 SS.reconfigure SSinhale.with maxSpeed: 72 @16:06:38.95 SS.seek 25ml @16:06:42.21 Vacuum too high -- waiting up to 40s @16:06:44.80 SS.reconfigure SSinhale.with maxSpeed: 65 @16:06:44.85 SS.seek 25ml @16:06:47.84 Vacuum too high -- waiting up to 40s @16:06:50.62 Intake @31.6psia, vacuum from 20.0 to 24.0psi @16:06:50.64 SS.reconfigure SSinhale.with maxSpeed: 59 @16:06:50.69 SS.seek 25ml @16:06:52.62 Stroke completed -- waiting for pressure to equalize ... @16:06:57.77 Intake.select :close @16:06:57.93 SSV.dial :exhaust @16:06:58.78 Exhaust.select :open @16:06:58.90 SS.reconfigure SSexhale @16:06:58.95 SS.seek 0ml @16:07:08.40 Exhaust.select :close @16:07:08.52 SSV.dial CBV @16:07:08.54 CBV.dial SSV @16:07:09.40 Allowing 130s for 25.0ml of remaining 1475.0ml @16:07:09.43 Intake.select :open @16:07:11.60 Intake @32.0psia, vacuum from 20.0 to 24.0psi @16:07:11.62 SS.reconfigure SSinhale.with maxSpeed: 59 @16:07:11.67 SS.seek 25ml @16:07:16.59 Vacuum too high -- waiting up to 40s @16:07:18.66 Intake @31.5psia, vacuum from 20.0 to 24.0psi @16:07:18.68 SS.reconfigure SSinhale.with maxSpeed: 53 @16:07:18.73 SS.seek 25ml @16:07:22.71 Vacuum too high -- waiting up to 40s @16:07:24.80 SS.reconfigure SSinhale.with maxSpeed: 48 @16:07:24.85 SS.seek 25ml @16:07:29.27 Vacuum too high -- waiting up to 40s @16:07:31.60 Intake @31.5psia, vacuum from 20.0 to 24.0psi @16:07:31.62 SS.reconfigure SSinhale.with maxSpeed: 43 @16:07:31.67 SS.seek 25ml @16:07:36.71 Vacuum too high -- waiting up to 40s @16:07:38.98 Intake @31.6psia, vacuum from 20.0 to 24.0psi @16:07:39.00 SS.reconfigure SSinhale.with maxSpeed: 39 @16:07:39.05 SS.seek 25ml @16:07:45.59 Vacuum too high -- waiting up to 40s @16:07:47.94 Intake @31.5psia, vacuum from 20.0 to 24.0psi @16:07:47.96 SS.reconfigure SSinhale.with maxSpeed: 35 @16:07:48.02 SS.seek 25ml @16:07:57.44 Vacuum too high -- waiting up to 40s @16:07:59.76 Intake @31.6psia, vacuum from 20.0 to 24.0psi @16:07:59.78 SS.reconfigure SSinhale.with maxSpeed: 32 @16:07:59.83 SS.seek 25ml @16:08:10.38 Vacuum too high -- waiting up to 40s @16:08:12.71 Intake @31.5psia, vacuum from 20.0 to 24.0psi @16:08:12.73 SS.reconfigure SSinhale.with maxSpeed: 29 @16:08:12.78 SS.seek 25ml @16:08:15.56 Stroke completed -- waiting for pressure to equalize ... @16:08:20.62 Intake.select :close @16:08:20.78 SSV.dial :exhaust @16:08:21.60 Exhaust.select :open @16:08:21.72 SS.reconfigure SSexhale @16:08:21.76 SS.seek 0ml @16:08:31.17 Exhaust.select :close @16:08:31.29 SSV.dial CBV @16:08:31.31 CBV.dial SSV @16:08:32.17 Allowing 130s for 25.0ml of remaining 1450.0ml @16:08:32.18 Intake.select :open @16:08:34.35 Intake @32.0psia, vacuum from 20.0 to 24.0psi @16:08:34.37 SS.reconfigure SSinhale.with maxSpeed: 29 @16:08:34.42 SS.seek 25ml @16:08:58.91 Vacuum too high -- waiting up to 40s @16:09:00.95 Intake @31.6psia, vacuum from 20.0 to 24.0psi @16:09:00.97 SS.reconfigure SSinhale.with maxSpeed: 26 @16:09:01.02 SS.seek 25ml @16:09:41.66 Stroke completed -- waiting for pressure to equalize ... @16:09:49.89 Intake.select :close @16:09:50.05 SSV.dial :exhaust @16:09:50.89 Exhaust.select :open @16:09:51.01 SS.reconfigure SSexhale @16:09:51.06 SS.seek 0ml @16:10:00.03 Exhaust.select :close @16:10:00.15 SSV.dial CBV @16:10:00.17 CBV.dial SSV @16:10:01.03 Allowing 130s for 25.0ml of remaining 1425.0ml @16:10:01.04 Intake.select :open @16:10:03.22 Intake @32.0psia, vacuum from 20.0 to 24.0psi @16:10:03.24 SS.reconfigure SSinhale.with maxSpeed: 26 @16:10:03.29 SS.seek 25ml @16:10:58.10 Vacuum too high -- waiting up to 40s @16:11:00.67 Intake @31.6psia, vacuum from 20.0 to 24.0psi @16:11:00.69 SS.reconfigure SSinhale.with maxSpeed: 23 @16:11:00.74 SS.seek 25ml @16:11:15.14 Stroke completed -- waiting for pressure to equalize ... @16:11:22.07 Intake.select :close @16:11:22.23 SSV.dial :exhaust @16:11:23.06 Exhaust.select :open @16:11:23.18 SS.reconfigure SSexhale @16:11:23.22 SS.seek 0ml @16:11:32.47 Exhaust.select :close @16:11:32.59 SSV.dial CBV @16:11:32.61 CBV.dial SSV @16:11:33.47 Allowing 130s for 25.0ml of remaining 1400.0ml @16:11:33.48 Intake.select :open @16:11:36.34 Intake @32.0psia, vacuum from 20.0 to 24.0psi @16:11:36.36 SS.reconfigure SSinhale.with maxSpeed: 23 @16:11:36.41 SS.seek 25ml @16:12:52.54 Stroke completed -- waiting for pressure to equalize ... @16:13:00.79 Intake.select :close @16:13:00.95 SSV.dial :exhaust @16:13:01.79 Exhaust.select :open @16:13:01.91 SS.reconfigure SSexhale @16:13:01.96 SS.seek 0ml @16:13:10.78 Exhaust.select :close @16:13:10.90 SSV.dial CBV @16:13:10.92 CBV.dial SSV @16:13:11.79 Allowing 130s for 25.0ml of remaining 1375.0ml @16:13:11.80 Intake.select :open @16:13:13.99 Intake @32.0psia, vacuum from 20.0 to 24.0psi @16:13:14.01 SS.reconfigure SSinhale.with maxSpeed: 23 @16:13:14.06 SS.seek 25ml @16:14:30.46 Stroke completed -- waiting for pressure to equalize ... @16:14:39.90 Intake.select :close @16:14:40.06 SSV.dial :exhaust @16:14:40.91 Exhaust.select :open @16:14:41.03 SS.reconfigure SSexhale @16:14:41.08 SS.seek 0ml @16:14:50.29 Exhaust.select :close @16:14:50.41 SSV.dial CBV @16:14:50.43 CBV.dial SSV @16:14:51.29 Allowing 130s for 25.0ml of remaining 1350.0ml @16:14:51.30 Intake.select :open @16:14:53.48 Intake @31.9psia, vacuum from 20.0 to 24.0psi @16:14:53.50 SS.reconfigure SSinhale.with maxSpeed: 23 @16:14:53.55 SS.seek 25ml @16:16:10.13 Stroke completed -- waiting for pressure to equalize ... @16:16:20.39 Intake.select :close @16:16:20.55 SSV.dial :exhaust @16:16:21.39 Exhaust.select :open @16:16:21.51 SS.reconfigure SSexhale @16:16:21.56 SS.seek 0ml @16:16:30.72 Exhaust.select :close @16:16:30.84 SSV.dial CBV @16:16:30.86 CBV.dial SSV @16:16:31.74 Allowing 130s for 25.0ml of remaining 1325.0ml @16:16:31.75 Intake.select :open @16:16:33.93 Intake @32.0psia, vacuum from 20.0 to 24.0psi @16:16:33.95 SS.reconfigure SSinhale.with maxSpeed: 23 @16:16:34.00 SS.seek 25ml @16:17:21.90 Vacuum too high -- waiting up to 40s @16:17:24.49 Intake @31.5psia, vacuum from 20.0 to 24.0psi @16:17:24.51 SS.reconfigure SSinhale.with maxSpeed: 21 @16:17:24.57 SS.seek 25ml @16:17:56.30 Stroke completed -- waiting for pressure to equalize ... @16:18:06.95 Intake.select :close @16:18:07.11 SSV.dial :exhaust @16:18:07.95 Exhaust.select :open @16:18:08.07 SS.reconfigure SSexhale @16:18:08.11 SS.seek 0ml @16:18:17.23 Exhaust.select :close @16:18:17.35 SSV.dial CBV @16:18:17.37 CBV.dial SSV @16:18:18.25 Allowing 130s for 25.0ml of remaining 1300.0ml @16:18:18.26 Intake.select :open @16:18:20.43 Intake @32.0psia, vacuum from 20.0 to 24.0psi @16:18:20.45 SS.reconfigure SSinhale.with maxSpeed: 21 @16:18:20.50 SS.seek 25ml @16:19:31.13 Vacuum too high -- waiting up to 40s @16:19:34.43 Intake @31.5psia, vacuum from 20.0 to 24.0psi @16:19:34.45 SS.reconfigure SSinhale.with maxSpeed: 19 @16:19:34.50 SS.seek 25ml @16:19:49.45 Stroke completed -- waiting for pressure to equalize ... @16:20:00.15 Intake.select :close @16:20:00.31 SSV.dial :exhaust @16:20:01.15 Exhaust.select :open @16:20:01.27 SS.reconfigure SSexhale @16:20:01.32 SS.seek 0ml @16:20:10.44 Exhaust.select :close @16:20:10.56 SSV.dial CBV @16:20:10.58 CBV.dial SSV @16:20:11.45 Allowing 130s for 25.0ml of remaining 1275.0ml @16:20:11.46 Intake.select :open @16:20:13.63 Intake @32.0psia, vacuum from 20.0 to 24.0psi @16:20:13.65 SS.reconfigure SSinhale.with maxSpeed: 19 @16:20:13.70 SS.seek 25ml @16:21:40.12 Vacuum too high -- waiting up to 40s @16:21:43.45 Intake @31.6psia, vacuum from 20.0 to 24.0psi @16:21:43.47 SS.reconfigure SSinhale.with maxSpeed: 17 @16:21:43.52 SS.seek 25ml @16:21:50.69 Stroke completed -- waiting for pressure to equalize ... @16:22:01.53 Intake.select :close @16:22:01.69 SSV.dial :exhaust @16:22:02.53 Exhaust.select :open @16:22:02.65 SS.reconfigure SSexhale @16:22:02.70 SS.seek 0ml @16:22:11.75 Exhaust.select :close @16:22:11.87 SSV.dial CBV @16:22:11.89 CBV.dial SSV @16:22:12.76 Allowing 130s for 25.0ml of remaining 1250.0ml @16:22:12.77 Intake.select :open @16:22:14.95 Intake @32.0psia, vacuum from 20.0 to 24.0psi @16:22:14.97 SS.reconfigure SSinhale.with maxSpeed: 17 @16:22:15.03 SS.seek 25ml @16:23:58.39 Stroke completed -- waiting for pressure to equalize ... @16:24:13.46 Intake.select :close @16:24:13.62 SSV.dial :exhaust @16:24:14.47 Exhaust.select :open @16:24:14.59 SS.reconfigure SSexhale @16:24:14.64 SS.seek 0ml @16:24:23.62 Exhaust.select :close @16:24:23.74 SSV.dial CBV @16:24:23.76 CBV.dial SSV @16:24:24.62 Allowing 130s for 25.0ml of remaining 1225.0ml @16:24:24.63 Intake.select :open @16:24:26.80 Intake @32.0psia, vacuum from 20.0 to 24.0psi @16:24:26.82 SS.reconfigure SSinhale.with maxSpeed: 17 @16:24:26.87 SS.seek 25ml @16:25:26.94 Vacuum too high -- waiting up to 40s @16:25:30.71 Intake @31.5psia, vacuum from 20.0 to 24.0psi @16:25:30.73 SS.reconfigure SSinhale.with maxSpeed: 15 @16:25:30.79 SS.seek 25ml @16:26:20.33 Stroke completed -- waiting for pressure to equalize ... @16:26:34.64 SS.reconfigure SSconfig @16:26:34.69 SS.hold @16:26:34.82 Intake.select :close @16:26:34.98 ShallowSampler::Clogged in WCR -- progress too slow after 300.0ml @16:26:34.99 SSV.dial :exhaust @16:26:35.83 Exhaust.select :open @16:26:35.95 SS.reconfigure SSexhale @16:26:36.00 SS.seek 0ml @16:26:45.41 Exhaust.select :close @16:26:45.52 Sampled 300.0ml @16:26:45.54 Drying puck before fixing for 4:00 @16:26:45.55 CBV.dial :waste2b @16:26:46.39 delay 1:00 @16:27:46.42 CSV.dial :air @16:27:47.23 CS.seek 10ml @16:28:12.13 Pumping wcr puck from tube 4 up to 55psi... @16:28:12.14 CBV.dial SSV @16:28:13.55 CBV.dial between CSV and waste2b @16:28:14.62 CSV.dial between air and CTV @16:28:14.95 CS.seek 7.154ml @16:28:22.77 CTV.dial CSV,avoiding: :air @16:28:22.79 CSV.dial CTV @16:28:23.69 CS.reconfigure CSpressurize @16:28:23.74 CS.seek 0ml @16:28:42.66 CSV.dial :air @16:28:43.18 CS.reconfigure CSfast @16:28:43.23 CS.seek 10ml @16:29:07.94 CSV.dial between air and CTV @16:29:09.00 CS.seek 2.556ml @16:29:27.86 CTV.dial CSV,avoiding: :air @16:29:27.88 CSV.dial CTV @16:29:28.24 CS.reconfigure CSpressurize @16:29:28.29 CS.seek 0ml @16:29:33.01 CBV.dial :waste2b @16:29:33.38 CS.reconfigure CSconfig @16:29:33.44 CS.seek :empty @16:29:36.98 delay 1:00 @16:30:37.01 CTV.dial :air,avoiding: :intake @16:30:38.46 Fix #1 of 2 (with rnal) for 2:00 @16:30:38.48 CSV.dial CSR @16:30:39.02 Pulling 2ml of CSR.rnal @16:30:39.04 CSR.select :rnal @16:30:39.16 CS.seek 2ml @16:30:45.29 delay 5 seconds @16:30:50.32 CSR.select :air @16:30:50.45 CS.reconfigure CSfast @16:30:50.50 CS.seek 10ml @16:31:10.51 Pumping wcr puck from tube 4 up to 55psi... @16:31:10.53 CBV.dial SSV @16:31:11.91 CBV.dial between CSV and waste2b @16:31:12.98 CSV.dial between air and CTV @16:31:13.64 CS.seek 7.219ml @16:31:21.30 CTV.dial CSV,avoiding: :air @16:31:21.32 CSV.dial CTV @16:31:22.89 CS.reconfigure CSpressurize @16:31:22.94 CS.seek 0ml @16:31:41.98 CSV.dial :air @16:31:42.49 CS.reconfigure CSfast @16:31:42.54 CS.seek 10ml @16:32:07.29 CSV.dial between air and CTV @16:32:07.66 CS.seek 2.174ml @16:32:27.35 CTV.dial CSV,avoiding: :air @16:32:27.38 CSV.dial CTV @16:32:27.74 CS.reconfigure CSpressurize @16:32:27.79 CS.seek 0ml @16:32:29.81 delay 2:00 @16:34:29.85 CBV.dial :waste2b @16:34:30.21 CS.reconfigure CSconfig @16:34:30.26 CS.seek :empty @16:34:35.27 delay 1:00 @16:35:35.30 CTV.dial :air,avoiding: :intake @16:35:36.75 Fix #2 of 2 (with rnal) for 2:00 @16:35:36.77 CSV.dial CSR @16:35:37.31 Pulling 2ml of CSR.rnal @16:35:37.33 CSR.select :rnal @16:35:37.45 CS.seek 2ml @16:35:43.67 delay 5 seconds @16:35:48.70 CSR.select :air @16:35:48.83 CS.reconfigure CSfast @16:35:48.88 CS.seek 10ml @16:36:08.83 Pumping wcr puck from tube 4 up to 55psi... @16:36:08.84 CBV.dial SSV @16:36:10.23 CBV.dial between CSV and waste2b @16:36:11.30 CSV.dial between air and CTV @16:36:12.76 CS.seek 7.245ml @16:36:20.43 CTV.dial CSV,avoiding: :air @16:36:20.46 CSV.dial CTV @16:36:21.96 CS.reconfigure CSpressurize @16:36:22.01 CS.seek 0ml @16:36:41.14 CSV.dial :air @16:36:41.67 CS.reconfigure CSfast @16:36:41.72 CS.seek 10ml @16:37:06.43 CSV.dial between air and CTV @16:37:06.79 CS.seek 2.231ml @16:37:26.34 CTV.dial CSV,avoiding: :air @16:37:26.36 CSV.dial CTV @16:37:26.71 CS.reconfigure CSpressurize @16:37:26.76 CS.seek 0ml @16:37:29.13 delay 2:00 @16:39:29.17 CBV.dial :waste2b @16:39:29.53 CS.reconfigure CSconfig @16:39:29.58 CS.seek :empty @16:39:34.43 delay 1:00 @16:40:34.46 CTV.dial :air,avoiding: :intake @16:40:35.98 Storing wcr puck in Collection Clamp to tube 3 @16:40:36.17 SC.reconfigure SCtight @16:40:36.22 SC.seek 3 @16:40:38.36 SC.reconfigure SCloose @16:40:38.54 SE.reconfigure SEplace @16:40:38.63 Elbow.reconfigure TightElbow @16:40:38.68 Elbow.seek CC @16:40:38.73 SE.seek beamBreak @16:40:38.87 Elbow.reconfigure LooseElbow @16:40:38.98 Forearm.seek CC @16:40:40.05 Hand.close @16:40:40.78 CC.seek :open @16:40:41.68 SE.configure SEconfig @16:40:41.74 SE.seek :place @16:40:45.28 Forearm.seek :retract @16:40:46.32 Elbow.reconfigure TightElbow @16:40:46.37 Elbow.seek 3 @16:40:48.28 Elbow.reconfigure LimpElbow @16:40:48.33 Forearm.seek 3 @16:40:49.36 Elbow.hold @16:40:49.44 Elbow.reconfigure TightElbow @16:40:49.49 Hand.open @16:40:50.42 Forearm.force -20 @16:40:50.47 Forearm.seek 3 + 333 counts @16:40:51.42 Forearm.reconfigure TightForearm @16:40:51.51 Forearm.seek 3 + 700 counts @16:40:51.84 SE.seek :down @16:40:53.00 Forearm.reconfigure LooseForearm @16:40:53.05 Forearm.seek :retract @16:40:54.60 SE.coast @16:40:54.64 SC.coast @16:40:54.69 Starting finalPurge @16:40:54.70 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:40:54.78 Hand.open @16:40:55.17 Elbow.seek FlushPuck::Garage @16:40:57.18 Elbow.reconfigure LooseElbow @16:40:57.28 Forearm.seek :garage @16:40:58.28 Hand.close @16:40:59.03 Forearm.seek :retract @16:41:00.08 Elbow.reconfigure TightElbow @16:41:00.13 Elbow.seek CC @16:41:02.99 Elbow.reconfigure LooseElbow @16:41:03.10 Forearm.reconfigure TightForearm @16:41:03.15 Forearm.seek CC @16:41:04.48 CC.seek :closed @16:41:08.74 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.05 @16:41:08.81 Hand.open @16:41:09.48 Forearm.reconfigure LooseForearm @16:41:09.52 Forearm.seek :retract @16:41:10.53 Flushing the Collection Valve::Manifold @16:41:10.67 CSV.dial CSR @16:41:11.21 Pulling 5ml of CSR.flush @16:41:11.22 CSR.select :flush @16:41:11.35 CS.seek 5ml @16:41:25.24 delay 5 seconds @16:41:30.27 CSR.select :air @16:41:30.44 CS.reconfigure CSfast @16:41:30.49 CS.seek 7.5ml @16:41:37.40 CSV.dial :waste @16:41:38.82 CS.reconfigure CSconfig @16:41:38.87 CS.seek 0ml @16:41:59.05 Flushing lower manifold's waste1 port @16:41:59.18 CSV.dial :flush @16:41:59.66 CS.reconfigure CSfast @16:41:59.71 CS.seek 5ml @16:42:12.62 delay 5 seconds @16:42:17.65 CSV.dial :air @16:42:18.33 CS.seek 7ml @16:42:24.05 CSV.dial CTV @16:42:24.09
CTV.dial CSV @16:42:25.62 CBV.dial :waste1 @16:42:26.47 CS.seek 0ml @16:42:44.08 Flushing lower manifold's waste2b port @16:42:44.21 CSV.dial :flush @16:42:45.05 CS.seek 5ml @16:42:58.58 delay 5 seconds @16:43:03.61 CSV.dial :air @16:43:04.19 CS.seek 7ml @16:43:09.93 CSV.dial CTV @16:43:09.97
CTV.dial CSV @16:43:10.44 CBV.dial :waste2b @16:43:11.28 CS.seek 0ml @16:43:28.87 Flushing upper manifold @16:43:29.01 CSV.dial :flush @16:43:29.83 CS.seek 5ml @16:43:42.68 delay 5 seconds @16:43:47.71 CSV.dial :air @16:43:48.28 CS.seek 7ml @16:43:54.02 CSV.dial CBV @16:43:54.05
CBV.dial CSV @16:43:55.23 CTV.dial :air @16:43:56.69 CS.seek 0ml @16:44:14.43 delay 7 seconds @16:44:21.47 CS.seek 3ml @16:44:29.68 CSV.dial :waste @16:44:30.17 CS.seek 0ml @16:44:38.39 CSV.dial :flush @16:44:38.88 CS.seek 3ml @16:44:47.12 delay 5 seconds @16:44:52.15 CSV.dial :air @16:44:52.73 CS.seek 5ml @16:44:58.43 CSV.dial :air @16:44:58.45 CS.seek 0ml @16:45:11.21 delay 7 seconds @16:45:18.25 CS.seek 3ml @16:45:26.37 CSV.dial :waste @16:45:27.18 CS.seek 0ml @16:45:35.34 Disinfecting external Intake with mfbkill @16:45:35.47 CSV.dial CSR @16:45:36.94 Pulling 8ml of CSR.mfbkill @16:45:36.95 CSR.select :mfbkill @16:45:37.07 CS.seek 8ml @16:45:57.15 delay 5 seconds @16:46:02.18 CSR.select :air @16:46:02.30 CS.seek 10ml @16:46:08.09 CSV.dial CBV @16:46:08.13
CBV.dial CSV @16:46:09.24 CTV.dial :intake @16:46:10.06 Intake.select :open @16:46:10.18 CS.seek 2ml @16:46:31.21 Intake.select :close @16:46:31.32 CTV.dial :air @16:46:32.16 CS.reconfigure CSconfig @16:46:32.21 CS.seek 0ml @16:46:38.25 Flushing lower manifold's waste1 port @16:46:38.26 CSV.dial CSR @16:46:39.45 Pulling 4ml of CSR.mfbkill @16:46:39.46 CSR.select :mfbkill @16:46:39.59 CS.seek 4ml @16:46:50.87 delay 5 seconds @16:46:55.90 CSR.select :air @16:46:56.03 CS.reconfigure CSfast @16:46:56.07 CS.seek 10ml @16:47:11.21 CSV.dial CTV @16:47:11.24
CTV.dial CSV @16:47:12.78 CBV.dial :waste1 @16:47:13.30 CS.seek 0ml @16:47:37.97 Flushing lower manifold's waste2b port @16:47:37.98 CSV.dial CSR @16:47:38.54 Pulling 4ml of CSR.mfbkill @16:47:38.55 CSR.select :mfbkill @16:47:38.67 CS.reconfigure CSconfig @16:47:38.71 CS.seek 4ml @16:47:49.93 delay 5 seconds @16:47:54.96 CSR.select :air @16:47:55.08 CS.reconfigure CSfast @16:47:55.13 CS.seek 10ml @16:48:10.31 CSV.dial CTV @16:48:10.35
CTV.dial CSV @16:48:11.10 CBV.dial :waste2b @16:48:11.96 CS.seek 0ml @16:48:36.56 Killing upper manifold @16:48:36.57 CSV.dial CSR @16:48:37.12 Pulling 4ml of CSR.mfbkill @16:48:37.13 CSR.select :mfbkill @16:48:37.26 CS.reconfigure CSconfig @16:48:37.31 CS.seek 4ml @16:48:48.53 delay 5 seconds @16:48:53.56 CSR.select :air @16:48:53.69 CS.reconfigure CSfast @16:48:53.73 CS.seek 10ml @16:49:08.90 CSV.dial CBV @16:49:08.93
CBV.dial CSV @16:49:10.09 CTV.dial :air @16:49:11.52 CS.seek 0ml @16:49:35.94 delay 7 seconds @16:49:42.98 CS.seek 3ml @16:49:51.06 CSV.dial :waste @16:49:51.56 CS.seek 0ml @16:49:59.75 Flushing upper manifold @16:49:59.88 CSV.dial :flush @16:50:00.37 CS.seek 5ml @16:50:13.16 delay 5 seconds @16:50:18.19 CSV.dial :air @16:50:18.87 CS.seek 7ml @16:50:24.69 CSV.dial CBV @16:50:24.72
CBV.dial CSV @16:50:25.86 CTV.dial :air @16:50:25.88 CS.seek 0ml @16:50:43.66 delay 7 seconds @16:50:50.70 CS.seek 3ml @16:50:58.75 CSV.dial :waste @16:50:59.24 CS.seek 0ml @16:51:07.62 CSV.dial :flush @16:51:08.12 CS.seek 3ml @16:51:16.13 delay 5 seconds @16:51:21.16 CSV.dial :air @16:51:21.71 CS.seek 5ml @16:51:27.41 CSV.dial :air @16:51:27.43 CS.seek 0ml @16:51:40.16 delay 7 seconds @16:51:47.20 CS.seek 3ml @16:51:55.38 CSV.dial :waste @16:51:56.20 CS.seek 0ml @16:52:04.28 Flushing lower manifold's waste1 port @16:52:04.41 CSV.dial :flush @16:52:04.92 CS.seek 5ml @16:52:17.78 delay 5 seconds @16:52:22.81 CSV.dial :air @16:52:23.37 CS.seek 7ml @16:52:29.10 CSV.dial CTV @16:52:29.14
CTV.dial CSV @16:52:30.67 CBV.dial :waste1 @16:52:31.17 CS.seek 0ml @16:52:48.63 Flushing lower manifold's waste2b port @16:52:48.76 CSV.dial :flush @16:52:49.59 CS.seek 5ml @16:53:02.32 delay 5 seconds @16:53:07.35 CSV.dial :air @16:53:07.92 CS.seek 7ml @16:53:13.63 CSV.dial CTV @16:53:13.67
CTV.dial CSV @16:53:14.14 CBV.dial :waste2b @16:53:14.98 CS.seek 0ml @16:53:32.69 Disinfecting external Intake with kill @16:53:32.82 CSV.dial CSR @16:53:33.36 Pulling 8ml of CSR.kill @16:53:33.37 CSR.select :kill @16:53:33.50 CS.seek 8ml @16:53:54.25 delay 5 seconds @16:53:59.28 CSR.select :air @16:53:59.41 CS.seek 10ml @16:54:05.13 CSV.dial CBV @16:54:05.16
CBV.dial CSV @16:54:06.32 CTV.dial :intake @16:54:07.14 Intake.select :open @16:54:07.26 CS.seek 2ml @16:54:28.29 Intake.select :close @16:54:28.40 CTV.dial :air @16:54:29.22 CS.reconfigure CSconfig @16:54:29.27 CS.seek 0ml @16:54:35.44 CSV.dial CSR @16:54:36.61 Pulling 5ml of CSR.flush @16:54:36.62 CSR.select :flush @16:54:36.75 CS.seek 5ml @16:54:50.85 delay 5 seconds @16:54:55.88 CSR.select :air @16:54:56.01 CS.seek 10ml @16:55:09.76 CSV.dial CBV @16:55:11.00 CS.seek 0ml @16:55:37.60 Garaging FlushPuck in Collection Clamp @16:55:37.68 Elbow.reconfigure TightElbow @16:55:37.73 Elbow.seek CC @16:55:37.97 Elbow.reconfigure LooseElbow @16:55:38.08 Forearm.seek CC @16:55:39.08 Hand.close @16:55:39.68 CC.seek :open @16:55:44.04 Forearm.seek :retract @16:55:45.02 Elbow.reconfigure TightElbow @16:55:45.07 Elbow.seek :garage @16:55:48.29 Elbow.reconfigure LooseElbow @16:55:48.39 Forearm.reconfigure TightForearm @16:55:48.44 Forearm.seek :garage @16:55:49.57 Hand.open @16:55:50.25 Forearm.reconfigure LooseForearm @16:55:50.30 Forearm.seek :retract @16:55:51.29 Starting initialPurge @16:55:51.30 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:55:51.38 Hand.open @16:55:51.75 Elbow.reconfigure TightElbow @16:55:51.80 Elbow.seek FlushPuck::Garage @16:55:51.99 Elbow.reconfigure LooseElbow @16:55:52.10 Forearm.seek :garage @16:55:53.08 Hand.close @16:55:53.81 Forearm.seek :retract @16:55:54.84 Elbow.reconfigure TightElbow @16:55:54.89 Elbow.seek CC @16:55:57.85 Elbow.reconfigure LooseElbow @16:55:57.95 Forearm.reconfigure TightForearm @16:55:58.00 Forearm.seek CC @16:55:59.31 CC.seek :closed @16:56:03.57 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 1.98 @16:56:03.64 Hand.open @16:56:04.37 Forearm.reconfigure LooseForearm @16:56:04.41 Forearm.seek :retract @16:56:05.42 Flushing the Collection Valve::Manifold @16:56:05.55 CSV.dial CSR @16:56:07.41 Pulling 5ml of CSR.flush @16:56:07.42 CSR.select :flush @16:56:07.54 CS.seek 5ml @16:56:21.32 delay 5 seconds @16:56:26.35 CSR.select :air @16:56:26.51 CS.reconfigure CSfast @16:56:26.56 CS.seek 7.5ml @16:56:33.44 CSV.dial :waste @16:56:34.85 CS.reconfigure CSconfig @16:56:34.90 CS.seek 0ml @16:56:55.01 Purging external Intake @16:56:55.14 CSV.dial :flush @16:56:55.63 CS.reconfigure CSfast @16:56:55.68 CS.seek 10ml @16:57:20.23 delay 5 seconds @16:57:25.26 CSV.dial CBV @16:57:25.31
CBV.dial CSV @16:57:26.10 CTV.dial :intake @16:57:26.93 Intake.select :open @16:57:27.05 CS.seek 0ml @16:57:53.35 Intake.select :close @16:57:53.46 CTV.dial :air @16:57:54.27 Flushing upper manifold @16:57:54.40 CSV.dial :flush @16:57:55.21 CS.seek 5ml @16:58:07.95 delay 5 seconds @16:58:12.98 CSV.dial :air @16:58:13.53 CS.seek 7ml @16:58:19.23 CSV.dial CBV @16:58:19.25
CBV.dial CSV @16:58:20.38 CTV.dial :air @16:58:20.40 CS.seek 0ml @16:58:37.92 delay 7 seconds @16:58:44.96 CS.seek 3ml @16:58:53.17 CSV.dial :waste @16:58:53.68 CS.seek 0ml @16:59:01.94 CSV.dial :flush @16:59:02.43 CS.seek 3ml @16:59:10.60 delay 5 seconds @16:59:15.63 CSV.dial :air @16:59:16.17 CS.seek 5.001ml @16:59:22.14 CSV.dial :air @16:59:22.16 CS.seek 0ml @16:59:34.95 delay 7 seconds @16:59:41.99 CS.seek 3ml @16:59:50.04 CSV.dial :waste @16:59:50.85 CS.seek 0ml @16:59:58.95 Garaging FlushPuck in Collection Clamp @16:59:59.03 Elbow.reconfigure TightElbow @16:59:59.08 Elbow.seek CC @16:59:59.32 Elbow.reconfigure LooseElbow @16:59:59.43 Forearm.seek CC @17:00:00.40 Hand.close @17:00:01.06 CC.seek :open @17:00:05.42 Forearm.seek :retract @17:00:06.44 Elbow.reconfigure TightElbow @17:00:06.49 Elbow.seek :garage @17:00:09.54 Elbow.reconfigure LooseElbow @17:00:09.65 Forearm.reconfigure TightForearm @17:00:09.69 Forearm.seek :garage @17:00:10.86 Hand.open @17:00:11.63 Forearm.reconfigure LooseForearm @17:00:11.67 Forearm.seek :retract @17:00:12.69 Completed [wcrphase] begun 16:00:25 @17:00:12.70 Preparing to sleep until 22:00 27-may-20 @17:00:12.77 SS.coast @17:00:12.79
Asleep til 22:00 27-may-20 @17:00:12.86 CC.coast @17:00:12.92
Continuing at 22:00 @17:00:12.95 CS.coast @17:00:13.06 PC.coast @17:00:13.09 GoodNews.email "Then, in tube 4: wcrphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:163\n{1 => 22, 2 => 22, 3 => 1, 4 => 21}\nCan@17:00:13, 9.8C, 49% humidity, 14.4psia, 12.984V, 0.462A, 0.462A avg, 6.00W",Subject: "Asleep until 22:00" @17:00:13.16 Forearm.coast @17:00:13.21 Elbow.coast @17:00:13.60 Gate.power :core,:OFF @17:00:13.61 Gate.power :raw,:OFF @17:00:13.63 Shutdown @22:00:00.05
Powering core bus @22:00:05.19 Gate.power :raw,:ON @22:00:05.86 SPE.coast @22:00:05.92 SPE.configure SPEconfig @22:00:06.02 AS.configure ASconfig @22:00:06.12 SS.configure SSconfig @22:00:06.22 CC.configure CCconfig @22:00:06.33 CS.configure CSconfig @22:00:06.43 PC.configure PCconfig @22:00:06.53 PS.configure PSconfig @22:00:06.65 Forearm.configure LooseForearm @22:00:06.75 Elbow.configure LooseElbow @22:00:06.87 SC.configure SCloose @22:00:06.97 SE.configure SEconfig @22:00:07.01 CH.coast @22:00:07.07 CH.configure CHconfig @22:00:07.10 PH.coast @22:00:07.16 PH.configure PHconfig @22:00:07.20 PCR.off @22:00:07.23 PCR.pidtemp= 50,0.1,0,300,25,25 @22:00:07.26 PCR.caltemp= 2.8814,0.0372,0 @22:00:07.29 All dwarf controllers running firmware version 3.75 @22:00:07.40 Valve.reset @22:00:07.57 PCR.off @22:00:07.61 PCR.pidtemp= 50,0.1,0,300,25,25 @22:00:07.64 PCR.caltemp= 2.8814,0.0372,0 @22:00:07.87 PSV.dial :air @22:00:07.97 Intake.select :close @22:00:08.00 PS.reconfigure PSconfig @22:00:08.04 Exhaust.select :close @22:00:08.11 CTV.dial :air @22:00:08.15 PS.home.seek 0ml @22:00:08.26 CSV.dial :waste1 @22:00:08.28
Forearm.reconfigure LooseForearm @22:00:08.40 Forearm.home.seek :retract @22:00:08.43 SSV.dial :spit,avoiding: :exhaust @22:00:09.20 Hand.close @22:00:09.24
Elbow.jog 1500 @22:00:09.36 CS.reconfigure CSfast @22:00:09.40 SS.reconfigure SSconfig @22:00:09.48 CS.home.seek 0ml @22:00:09.52 SS.home.seek 0ml @22:00:09.92
Elbow.reconfigure LooseElbow @22:00:09.97 Elbow.home.seek :clear @22:00:13.39 SE.reconfigure SEconfig @22:00:13.43 SE.home.seek :down @22:00:14.16 SC.reconfigure SCsloppy @22:00:14.21 SC.home.seek :initial @22:00:17.16 PS.coast @22:00:19.81
Forearm.seek :retract @22:00:19.89 Elbow.seek :hammer @22:00:21.20 Forearm.reconfigure TightForearm @22:00:21.26 Forearm.seek 5 + 350 counts @22:00:22.33 Forearm.seek :retract @22:00:24.23 PC.reconfigure PCconfig @22:00:24.24
CC.reconfigure CCconfig @22:00:24.33 CC.home.seek :open @22:00:24.37 PC.home.seek :open @22:00:27.88
Phase [wcrphase] @22:00:27.90 GoodNews.email "At 22:00:27 began in tube 4: wcrphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:163\n{1 => 22, 2 => 22, 3 => 1, 4 => 21}\nCan@22:00:27, 9.2C, 50% humidity, 14.4psia, 13.043V, 0.474A, 0.474A avg, 6.19W",Subject: "Started 20invasivedeployfinal phase wcrphase" @22:00:27.92CEST27-May-20
===> STARTED WCRSphase (phase #19) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @22:00:27.95 Starting initialPurge @22:00:27.97 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:00:28.04 Hand.open @22:00:28.83 Elbow.reconfigure TightElbow @22:00:28.88 Elbow.seek FlushPuck::Garage @22:00:30.82 Elbow.reconfigure LooseElbow @22:00:31.09 Forearm.reconfigure LooseForearm @22:00:31.19 Forearm.seek :garage @22:00:32.26 Hand.close @22:00:32.98 Forearm.seek :retract @22:00:34.01 Elbow.reconfigure TightElbow @22:00:34.10 Elbow.seek CC @22:00:37.20 Elbow.reconfigure LooseElbow @22:00:37.44 Forearm.reconfigure TightForearm @22:00:37.53 Forearm.seek CC @22:00:39.27 CC.seek :closed @22:00:43.99 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.25 @22:00:44.18 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[07:27->20] @22:00:44.29 Hand.open @22:00:45.11 Forearm.reconfigure LooseForearm @22:00:45.24 Forearm.seek :retract @22:00:46.30 Flushing the Collection Valve::Manifold @22:00:46.61 CSV.dial CSR @22:00:48.25 Pulling 5ml of CSR.flush @22:00:48.26 CSR.select :flush @22:00:48.43 CS.reconfigure CSconfig @22:00:48.53 CS.seek 5ml @22:01:02.37 delay 5 seconds @22:01:07.46 CSR.select :air @22:01:07.76 CS.reconfigure CSfast @22:01:07.86 CS.seek 7.5ml @22:01:14.92 CSV.dial :waste @22:01:16.40 CS.reconfigure CSconfig @22:01:16.50 CS.seek 0ml @22:01:36.68 Purging external Intake @22:01:36.81 CSV.dial :flush @22:01:37.31 CS.reconfigure CSfast @22:01:37.36 CS.seek 10ml @22:02:02.00 delay 5 seconds @22:02:07.03 CSV.dial CBV @22:02:07.10
CBV.dial CSV @22:02:07.92 CTV.dial :intake @22:02:08.75 Intake.select :open @22:02:08.87 CS.seek 0ml @22:02:35.06 Intake.select :close @22:02:35.17 CTV.dial :air @22:02:36.00 Flushing upper manifold @22:02:36.14 CSV.dial :flush @22:02:36.94 CS.seek 5ml @22:02:49.81 delay 5 seconds @22:02:55.50 CSV.dial :air @22:02:56.05 CS.seek 7ml @22:03:02.00 CSV.dial CBV @22:03:02.03
CBV.dial CSV @22:03:03.15 CTV.dial :air @22:03:03.17 CS.seek 0ml @22:03:20.65 delay 7 seconds @22:03:27.69 CS.seek 3ml @22:03:35.81 CSV.dial :waste @22:03:36.30 CS.seek 0ml @22:03:44.55 CSV.dial :flush @22:03:45.04 CS.seek 3ml @22:03:53.18 delay 5 seconds @22:03:58.21 CSV.dial :air @22:03:58.76 CS.seek 5ml @22:04:04.47 CSV.dial :air @22:04:04.49 CS.seek 0ml @22:04:17.28 delay 7 seconds @22:04:24.32 CS.seek 3ml @22:04:32.37 CSV.dial :waste @22:04:33.18 CS.seek 0ml @22:04:41.31 Garaging FlushPuck in Collection Clamp @22:04:41.39 Elbow.reconfigure TightElbow @22:04:41.44 Elbow.seek CC @22:04:41.72 Elbow.reconfigure LooseElbow @22:04:41.83 Forearm.seek CC @22:04:42.82 Hand.close @22:04:43.58 CC.seek :open @22:04:47.94 Forearm.seek :retract @22:04:48.92 Elbow.reconfigure TightElbow @22:04:48.97 Elbow.seek :garage @22:04:52.16 Elbow.reconfigure LooseElbow @22:04:52.26 Forearm.reconfigure TightForearm @22:04:52.31 Forearm.seek :garage @22:04:53.44 Hand.open @22:04:54.28 Forearm.reconfigure LooseForearm @22:04:54.32 Forearm.seek :retract @22:04:55.33 Starting stand-alone bac WCR @22:04:55.34 bac wcr sampling at most 1500ml @22:04:55.37 Whole Cell Archive sampling 1500ml @22:04:55.39 BACfans.email "Started stand-alone bac WCR in tube 4 at 22:04:55CEST27-May-20\n{1 => 22, 2 => 22, 3 => 1, 4 => 21}\nCan@22:00:27, 9.2C, 50% humidity, 14.4psia, 13.043V, 0.474A, 0.474A avg, 6.19W",Subject: "bac wcr sampling at most 1500ml" @22:04:55.41 Loading wcr puck from tube 4 into Collection Clamp @22:04:55.72 SC.reconfigure SCtight @22:04:55.78 SC.seek 4 @22:04:59.48 SC.reconfigure SCloose @22:04:59.73 Elbow.reconfigure TightElbow @22:04:59.78 Elbow.seek 4 @22:05:02.21 Forearm.reconfigure TightForearm @22:05:02.32 Forearm.seek 4 + 700 counts @22:05:03.59 SE.reconfigure SEpick @22:05:05.29 SE.seek beamBreak @22:05:06.67 SE.configure SEconfig @22:05:07.10 Elbow.seek 4 @22:05:07.62 Forearm.seek 4 + 300 counts @22:05:07.91 Forearm.reconfigure LooseForearm @22:05:08.00 Hand.close @22:05:08.79 SE.seek :down @22:05:09.32 Forearm.seek :retract @22:05:09.83 SE.coast @22:05:10.38 Elbow.seek CC @22:05:12.21 Elbow.reconfigure LooseElbow @22:05:12.47 Forearm.reconfigure TightForearm @22:05:12.57 Forearm.seek CC @22:05:14.35 CC.seek :closed @22:05:18.84 Collection Clamp onSpring/offSpring for 6/30 samples, on/off torque 2.31 @22:05:18.89 Hand.open @22:05:19.76 Forearm.reconfigure LooseForearm @22:05:19.87 Forearm.seek :retract @22:05:20.96 SC.coast @22:05:21.08 collectSample 1500ml w/bubble point of 55psi @22:05:21.11 Priming 7ml @22:05:21.13 Intake.select :close @22:05:21.15 Exhaust.select :close @22:05:21.18 SS.hold @22:05:21.67 Calibrating pressure and temperature sensors @22:05:21.71 CTV.dial :air @22:05:21.73 SSV.dial :spit,avoiding: :exhaust @22:05:21.77 SS.reconfigure SSfast @22:05:21.92 SS.seek 5ml @22:05:24.88 SSV.dial CBV @22:05:24.95 CBV.dial SSV @22:05:26.50 delay 3.8 seconds @22:05:30.62 SSV.dial :spit @22:05:32.13 SS.seek 0ml @22:05:34.71 SSV.dial CBV @22:05:36.19 CTV.dial :intake @22:05:37.08 SSV.dial CBV @22:05:37.15 CBV.dial SSV @22:05:37.18 Allowing 40s for 7.0ml of remaining 7.0ml @22:05:37.21 Intake.select :open @22:05:39.53 Intake @31.2psia, vacuum from 20.0 to 24.0psi @22:05:39.57 SS.reconfigure SSinhale.with maxSpeed: 150 @22:05:39.67 SS.seek 7ml @22:05:43.97 Vacuum too high -- waiting up to 40s @22:05:49.20 Intake @31.2psia, vacuum from 20.0 to 24.0psi @22:05:49.28 SS.reconfigure SSinhale.with maxSpeed: 135 @22:05:49.37 SS.seek 7ml @22:05:51.94 Stroke completed -- waiting for pressure to equalize ... @22:05:56.81 Intake.select :close @22:05:57.05 SSV.dial :exhaust @22:05:57.94 Exhaust.select :open @22:05:58.12 SS.reconfigure SSexhale @22:05:58.22 SS.seek 0ml @22:06:02.10 Exhaust.select :close @22:06:02.26 Sampling 1500.0ml @22:06:02.29 Intake.select :close @22:06:02.31 Exhaust.select :close @22:06:02.34 SS.reconfigure SSconfig @22:06:02.42 SS.hold @22:06:02.87 CTV.dial :intake @22:06:02.92 SSV.dial CBV @22:06:02.97 CBV.dial SSV @22:06:03.88 Allowing 130s for 25.0ml of remaining 1500.0ml @22:06:03.92 Intake.select :open @22:06:06.20 Intake @31.4psia, vacuum from 20.0 to 24.0psi @22:06:06.24 SS.reconfigure SSinhale.with maxSpeed: 135 @22:06:06.33 SS.seek 25ml @22:06:09.97 Vacuum too high -- waiting up to 40s @22:06:12.43 Intake @31.1psia, vacuum from 20.0 to 24.0psi @22:06:12.45 SS.reconfigure SSinhale.with maxSpeed: 122 @22:06:12.50 SS.seek 25ml @22:06:15.57 Vacuum too high -- waiting up to 40s @22:06:18.16 Intake @31.2psia, vacuum from 20.0 to 24.0psi @22:06:18.18 SS.reconfigure SSinhale.with maxSpeed: 110 @22:06:18.23 SS.seek 25ml @22:06:21.10 Vacuum too high -- waiting up to 40s @22:06:23.69 Intake @31.2psia, vacuum from 20.0 to 24.0psi @22:06:23.71 SS.reconfigure SSinhale.with maxSpeed: 99 @22:06:24.46 SS.seek 25ml @22:06:27.71 Vacuum too high -- waiting up to 40s @22:06:30.08 SS.reconfigure SSinhale.with maxSpeed: 89 @22:06:30.14 SS.seek 25ml @22:06:32.94 Vacuum too high -- waiting up to 40s @22:06:35.28 Intake @31.2psia, vacuum from 20.0 to 24.0psi @22:06:35.30 SS.reconfigure SSinhale.with maxSpeed: 80 @22:06:35.35 SS.seek 25ml @22:06:38.09 Vacuum too high -- waiting up to 40s @22:06:40.43 SS.reconfigure SSinhale.with maxSpeed: 72 @22:06:40.48 SS.seek 25ml @22:06:43.37 Vacuum too high -- waiting up to 40s @22:06:45.69 SS.reconfigure SSinhale.with maxSpeed: 65 @22:06:45.74 SS.seek 25ml @22:06:48.48 Vacuum too high -- waiting up to 40s @22:06:50.82 SS.reconfigure SSinhale.with maxSpeed: 59 @22:06:50.87 SS.seek 25ml @22:06:54.69 Vacuum too high -- waiting up to 40s @22:06:56.55 Intake @31.3psia, vacuum from 20.0 to 24.0psi @22:06:56.57 SS.reconfigure SSinhale.with maxSpeed: 53 @22:06:56.62 SS.seek 25ml @22:06:58.06 Stroke completed -- waiting for pressure to equalize ... @22:07:01.91 Intake.select :close @22:07:02.07 SSV.dial :exhaust @22:07:02.89 Exhaust.select :open @22:07:03.01 SS.reconfigure SSexhale @22:07:03.06 SS.seek 0ml @22:07:11.84 Exhaust.select :close @22:07:11.96 SSV.dial CBV @22:07:11.98 CBV.dial SSV @22:07:12.83 Allowing 130s for 25.0ml of remaining 1475.0ml @22:07:12.84 Intake.select :open @22:07:15.02 Intake @31.5psia, vacuum from 20.0 to 24.0psi @22:07:15.04 SS.reconfigure SSinhale.with maxSpeed: 53 @22:07:15.09 SS.seek 25ml @22:07:21.21 Vacuum too high -- waiting up to 40s @22:07:23.53 Intake @31.2psia, vacuum from 20.0 to 24.0psi @22:07:23.55 SS.reconfigure SSinhale.with maxSpeed: 48 @22:07:23.60 SS.seek 25ml @22:07:28.70 Vacuum too high -- waiting up to 40s @22:07:31.03 Intake @31.3psia, vacuum from 20.0 to 24.0psi @22:07:31.05 SS.reconfigure SSinhale.with maxSpeed: 43 @22:07:31.10 SS.seek 25ml @22:07:37.04 Vacuum too high -- waiting up to 40s @22:07:40.39 Intake @31.4psia, vacuum from 20.0 to 24.0psi @22:07:40.41 SS.reconfigure SSinhale.with maxSpeed: 39 @22:07:40.46 SS.seek 25ml @22:07:48.27 Vacuum too high -- waiting up to 40s @22:07:50.65 Intake @31.3psia, vacuum from 20.0 to 24.0psi @22:07:50.67 SS.reconfigure SSinhale.with maxSpeed: 35 @22:07:50.72 SS.seek 25ml @22:07:58.71 Vacuum too high -- waiting up to 40s @22:08:01.29 Intake @31.2psia, vacuum from 20.0 to 24.0psi @22:08:01.31 SS.reconfigure SSinhale.with maxSpeed: 32 @22:08:01.36 SS.seek 25ml @22:08:13.14 Vacuum too high -- waiting up to 40s @22:08:15.98 Intake @31.3psia, vacuum from 20.0 to 24.0psi @22:08:16.00 SS.reconfigure SSinhale.with maxSpeed: 29 @22:08:16.05 SS.seek 25ml @22:08:21.16 Stroke completed -- waiting for pressure to equalize ... @22:08:28.11 Intake.select :close @22:08:28.27 SSV.dial :exhaust @22:08:29.11 Exhaust.select :open @22:08:29.23 SS.reconfigure SSexhale @22:08:29.27 SS.seek 0ml @22:08:38.50 Exhaust.select :close @22:08:38.66 SSV.dial CBV @22:08:38.68 CBV.dial SSV @22:08:39.55 Allowing 130s for 25.0ml of remaining 1450.0ml @22:08:39.56 Intake.select :open @22:08:42.44 Intake @31.4psia, vacuum from 20.0 to 24.0psi @22:08:42.46 SS.reconfigure SSinhale.with maxSpeed: 29 @22:08:42.51 SS.seek 25ml @22:09:01.36 Vacuum too high -- waiting up to 40s @22:09:03.98 Intake @31.2psia, vacuum from 20.0 to 24.0psi @22:09:04.00 SS.reconfigure SSinhale.with maxSpeed: 26 @22:09:04.05 SS.seek 25ml @22:09:29.70 Vacuum too high -- waiting up to 40s @22:09:32.55 SS.reconfigure SSinhale.with maxSpeed: 23 @22:09:32.60 SS.seek 25ml @22:09:57.12 Stroke completed -- waiting for pressure to equalize ... @22:10:06.56 Intake.select :close @22:10:06.72 SSV.dial :exhaust @22:10:07.56 Exhaust.select :open @22:10:07.68 SS.reconfigure SSexhale @22:10:07.72 SS.seek 0ml @22:10:16.64 Exhaust.select :close @22:10:16.76 SSV.dial CBV @22:10:16.78 CBV.dial SSV @22:10:17.63 Allowing 130s for 25.0ml of remaining 1425.0ml @22:10:17.64 Intake.select :open @22:10:19.82 Intake @31.4psia, vacuum from 20.0 to 24.0psi @22:10:19.84 SS.reconfigure SSinhale.with maxSpeed: 23 @22:10:19.89 SS.seek 25ml @22:11:19.58 Vacuum too high -- waiting up to 40s @22:11:22.87 Intake @31.2psia, vacuum from 20.0 to 24.0psi @22:11:22.89 SS.reconfigure SSinhale.with maxSpeed: 21 @22:11:22.94 SS.seek 25ml @22:11:41.48 Stroke completed -- waiting for pressure to equalize ... @22:11:51.57 Intake.select :close @22:11:51.73 SSV.dial :exhaust @22:11:52.55 Exhaust.select :open @22:11:52.67 SS.reconfigure SSexhale @22:11:52.72 SS.seek 0ml @22:12:01.92 Exhaust.select :close @22:12:02.04 SSV.dial CBV @22:12:02.06 CBV.dial SSV @22:12:02.91 Allowing 130s for 25.0ml of remaining 1400.0ml @22:12:02.92 Intake.select :open @22:12:05.10 Intake @31.4psia, vacuum from 20.0 to 24.0psi @22:12:05.12 SS.reconfigure SSinhale.with maxSpeed: 21 @22:12:05.17 SS.seek 25ml @22:13:28.23 Stroke completed -- waiting for pressure to equalize ... @22:13:41.39 Intake.select :close @22:13:41.55 SSV.dial :exhaust @22:13:42.39 Exhaust.select :open @22:13:42.51 SS.reconfigure SSexhale @22:13:42.56 SS.seek 0ml @22:13:51.42 Exhaust.select :close @22:13:52.24 SSV.dial CBV @22:13:52.26 CBV.dial SSV @22:13:53.11 Allowing 130s for 25.0ml of remaining 1375.0ml @22:13:53.12 Intake.select :open @22:13:55.31 Intake @31.4psia, vacuum from 20.0 to 24.0psi @22:13:55.33 SS.reconfigure SSinhale.with maxSpeed: 21 @22:13:55.37 SS.seek 25ml @22:14:59.34 Vacuum too high -- waiting up to 40s @22:15:02.93 Intake @31.0psia, vacuum from 20.0 to 24.0psi @22:15:02.95 SS.reconfigure SSinhale.with maxSpeed: 19 @22:15:03.00 SS.seek 25ml @22:15:24.94 Stroke completed -- waiting for pressure to equalize ... @22:15:37.45 Intake.select :close @22:15:37.61 SSV.dial :exhaust @22:15:38.46 Exhaust.select :open @22:15:38.58 SS.reconfigure SSexhale @22:15:38.62 SS.seek 0ml @22:15:47.67 Exhaust.select :close @22:15:47.79 SSV.dial CBV @22:15:47.81 CBV.dial SSV @22:15:48.67 Allowing 130s for 25.0ml of remaining 1350.0ml @22:15:48.68 Intake.select :open @22:15:50.85 Intake @31.4psia, vacuum from 20.0 to 24.0psi @22:15:50.87 SS.reconfigure SSinhale.with maxSpeed: 19 @22:15:50.92 SS.seek 25ml @22:17:23.29 Stroke completed -- waiting for pressure to equalize ... @22:17:39.10 Intake.select :close @22:17:39.26 SSV.dial :exhaust @22:17:40.10 Exhaust.select :open @22:17:40.22 SS.reconfigure SSexhale @22:17:40.27 SS.seek 0ml @22:17:49.55 Exhaust.select :close @22:17:49.67 SSV.dial CBV @22:17:49.69 CBV.dial SSV @22:17:50.57 Allowing 130s for 25.0ml of remaining 1325.0ml @22:17:50.58 Intake.select :open @22:17:52.75 Intake @31.3psia, vacuum from 20.0 to 24.0psi @22:17:52.77 SS.reconfigure SSinhale.with maxSpeed: 19 @22:17:52.82 SS.seek 25ml @22:18:46.18 Vacuum too high -- waiting up to 40s @22:18:49.75 Intake @31.1psia, vacuum from 20.0 to 24.0psi @22:18:49.77 SS.reconfigure SSinhale.with maxSpeed: 17 @22:18:49.82 SS.seek 25ml @22:19:34.09 Stroke completed -- waiting for pressure to equalize ... @22:19:48.60 Intake.select :close @22:19:48.76 SSV.dial :exhaust @22:19:49.60 Exhaust.select :open @22:19:49.72 SS.reconfigure SSexhale @22:19:49.78 SS.seek 0ml @22:19:58.75 Exhaust.select :close @22:19:58.87 SSV.dial CBV @22:19:58.89 CBV.dial SSV @22:19:59.74 Allowing 130s for 25.0ml of remaining 1300.0ml @22:19:59.75 Intake.select :open @22:20:01.94 Intake @31.4psia, vacuum from 20.0 to 24.0psi @22:20:01.95 SS.reconfigure SSinhale.with maxSpeed: 17 @22:20:02.00 SS.seek 25ml @22:21:14.37 Vacuum too high -- waiting up to 40s @22:21:18.76 Intake @31.1psia, vacuum from 20.0 to 24.0psi @22:21:18.78 SS.reconfigure SSinhale.with maxSpeed: 15 @22:21:18.83 SS.seek 25ml @22:21:54.51 Stroke completed -- waiting for pressure to equalize ... @22:22:10.19 SS.reconfigure SSconfig @22:22:10.25 SS.hold @22:22:10.38 Intake.select :close @22:22:10.54 ShallowSampler::Clogged in WCR -- progress too slow after 225.0ml @22:22:10.56 SSV.dial :exhaust @22:22:11.39 Exhaust.select :open @22:22:11.51 SS.reconfigure SSexhale @22:22:11.56 SS.seek 0ml @22:22:20.61 Exhaust.select :close @22:22:20.72 Sampled 225.0ml @22:22:20.74 Drying puck before fixing for 4:00 @22:22:20.75 CBV.dial :waste2b @22:22:21.60 delay 1:00 @22:23:21.64 CSV.dial :air @22:23:22.45 CS.seek 10ml @22:23:47.40 Pumping wcr puck from tube 4 up to 55psi... @22:23:47.42 CBV.dial SSV @22:23:48.81 CBV.dial between CSV and waste2b @22:23:49.89 CSV.dial between air and CTV @22:23:50.23 CS.seek 7.159ml @22:23:58.22 CTV.dial CSV,avoiding: :air @22:23:58.24 CSV.dial CTV @22:23:59.15 CS.reconfigure CSpressurize @22:23:59.21 CS.seek 0ml @22:24:18.12 CSV.dial :air @22:24:18.64 CS.reconfigure CSfast @22:24:18.69 CS.seek 10ml @22:24:43.49 CSV.dial between air and CTV @22:24:43.86 CS.seek 2.534ml @22:25:02.72 CTV.dial CSV,avoiding: :air @22:25:02.74 CSV.dial CTV @22:25:03.09 CS.reconfigure CSpressurize @22:25:03.14 CS.seek 0ml @22:25:07.67 CBV.dial :waste2b @22:25:08.05 CS.reconfigure CSconfig @22:25:08.10 CS.seek :empty @22:25:11.68 delay 1:00 @22:26:11.71 CTV.dial :air,avoiding: :intake @22:26:13.16 Fix #1 of 2 (with rnal) for 2:00 @22:26:13.18 CSV.dial CSR @22:26:13.72 Pulling 2ml of CSR.rnal @22:26:13.74 CSR.select :rnal @22:26:13.86 CS.seek 2ml @22:26:20.02 delay 5 seconds @22:26:25.05 CSR.select :air @22:26:25.18 CS.reconfigure CSfast @22:26:25.23 CS.seek 10ml @22:26:45.15 Pumping wcr puck from tube 4 up to 55psi... @22:26:45.17 CBV.dial SSV @22:26:46.55 CBV.dial between CSV and waste2b @22:26:47.63 CSV.dial between air and CTV @22:26:48.30 CS.seek 7.224ml @22:26:56.07 CTV.dial CSV,avoiding: :air @22:26:56.09 CSV.dial CTV @22:26:57.63 CS.reconfigure CSpressurize @22:26:58.36 CS.seek 0ml @22:27:17.46 CSV.dial :air @22:27:17.97 CS.reconfigure CSfast @22:27:18.02 CS.seek 10ml @22:27:42.80 CSV.dial between air and CTV @22:27:43.16 CS.seek 2.143ml @22:28:03.07 CTV.dial CSV,avoiding: :air @22:28:03.09 CSV.dial CTV @22:28:03.44 CS.reconfigure CSpressurize @22:28:03.49 CS.seek 0ml @22:28:05.36 delay 2:00 @22:30:05.40 CBV.dial :waste2b @22:30:05.75 CS.reconfigure CSconfig @22:30:05.80 CS.seek :empty @22:30:11.13 delay 1:00 @22:31:11.16 CTV.dial :air,avoiding: :intake @22:31:12.61 Fix #2 of 2 (with rnal) for 2:00 @22:31:12.63 CSV.dial CSR @22:31:13.17 Pulling 2ml of CSR.rnal @22:31:13.18 CSR.select :rnal @22:31:13.31 CS.seek 2ml @22:31:19.62 delay 5 seconds @22:31:24.65 CSR.select :air @22:31:24.78 CS.reconfigure CSfast @22:31:24.83 CS.seek 10ml @22:31:44.81 Pumping wcr puck from tube 4 up to 55psi... @22:31:44.83 CBV.dial SSV @22:31:46.22 CBV.dial between CSV and waste2b @22:31:47.27 CSV.dial between air and CTV @22:31:48.06 CS.seek 7.224ml @22:31:55.79 CTV.dial CSV,avoiding: :air @22:31:55.81 CSV.dial CTV @22:31:57.34 CS.reconfigure CSpressurize @22:31:57.39 CS.seek 0ml @22:32:16.44 CSV.dial :air @22:32:16.95 CS.reconfigure CSfast @22:32:17.00 CS.seek 10ml @22:32:41.78 CSV.dial between air and CTV @22:32:42.15 CS.seek 2.222ml @22:33:01.82 CTV.dial CSV,avoiding: :air @22:33:01.84 CSV.dial CTV @22:33:02.20 CS.reconfigure CSpressurize @22:33:02.25 CS.seek 0ml @22:33:04.52 delay 2:00 @22:35:04.56 CBV.dial :waste2b @22:35:04.91 CS.reconfigure CSconfig @22:35:04.96 CS.seek :empty @22:35:09.86 delay 1:00 @22:36:09.89 CTV.dial :air,avoiding: :intake @22:36:11.42 Storing wcr puck in Collection Clamp to tube 3 @22:36:11.61 SC.reconfigure SCtight @22:36:11.66 SC.seek 3 @22:36:13.79 SC.reconfigure SCloose @22:36:14.68 Elbow.reconfigure TightElbow @22:36:14.74 Elbow.seek 3 @22:36:16.67 Forearm.reconfigure TightForearm @22:36:16.72 Forearm.seek 3 + 700 counts @22:36:17.81 SE.reconfigure SEpick @22:36:17.90 SE.seek beamBreak @22:36:20.96 SE.configure SEconfig @22:36:21.13 Elbow.reconfigure LimpElbow @22:36:21.18 Forearm.reconfigure LooseForearm @22:36:21.23 Forearm.seek 3 @22:36:21.49 Elbow.hold @22:36:21.57 Elbow.reconfigure TightElbow @22:36:21.68 SE.seek 0.07 Pucks below Top Plate @22:36:22.14 Forearm.force -20 @22:36:22.19 Forearm.seek 3 + 347 counts @22:36:22.51 Forearm.reconfigure TightForearm @22:36:22.56 Forearm.seek 3 + 700 counts @22:36:22.90 SE.seek 1.07 Pucks below Top Plate @22:36:23.49 Forearm.reconfigure LooseForearm @22:36:23.54 Forearm.seek :retract @22:36:24.48 Elbow.seek CC @22:36:26.26 Elbow.reconfigure LooseElbow @22:36:26.37 Forearm.seek CC @22:36:27.43 Hand.close @22:36:28.14 CC.seek :open @22:36:32.67 Forearm.seek :retract @22:36:33.70 Elbow.reconfigure TightElbow @22:36:33.75 Elbow.seek 3 @22:36:35.97 Elbow.reconfigure LimpElbow @22:36:36.02 Forearm.seek 3 @22:36:37.06 Elbow.hold @22:36:37.13 Elbow.reconfigure TightElbow @22:36:37.19 Hand.open @22:36:37.91 Forearm.force -20 @22:36:37.95 Forearm.seek 3 + 336 counts @22:36:38.27 Forearm.reconfigure TightForearm @22:36:38.33 Forearm.seek 3 + 700 counts @22:36:38.64 SE.seek :down @22:36:39.80 Forearm.reconfigure LooseForearm @22:36:39.85 Forearm.seek :retract @22:36:41.40 SE.coast @22:36:41.44 SC.coast @22:36:42.17 Starting finalPurge @22:36:42.18 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:36:42.26 Hand.open @22:36:42.65 Elbow.seek FlushPuck::Garage @22:36:44.63 Elbow.reconfigure LooseElbow @22:36:44.74 Forearm.seek :garage @22:36:45.75 Hand.close @22:36:46.52 Forearm.seek :retract @22:36:47.51 Elbow.reconfigure TightElbow @22:36:47.56 Elbow.seek CC @22:36:50.51 Elbow.reconfigure LooseElbow @22:36:50.62 Forearm.reconfigure TightForearm @22:36:50.66 Forearm.seek CC @22:36:51.98 CC.seek :closed @22:36:56.25 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.10 @22:36:56.32 Hand.open @22:36:56.96 Forearm.reconfigure LooseForearm @22:36:57.01 Forearm.seek :retract @22:36:58.00 Flushing the Collection Valve::Manifold @22:36:58.14 CSV.dial CSR @22:36:58.67 Pulling 5ml of CSR.flush @22:36:58.68 CSR.select :flush @22:36:58.81 CS.seek 5ml @22:37:12.63 delay 5 seconds @22:37:17.66 CSR.select :air @22:37:17.83 CS.reconfigure CSfast @22:37:17.88 CS.seek 7.501ml @22:37:24.79 CSV.dial :waste @22:37:26.21 CS.reconfigure CSconfig @22:37:26.26 CS.seek 0ml @22:37:46.35 Flushing lower manifold's waste1 port @22:37:46.48 CSV.dial :flush @22:37:47.08 CS.reconfigure CSfast @22:37:47.14 CS.seek 5ml @22:38:00.01 delay 5 seconds @22:38:05.04 CSV.dial :air @22:38:05.61 CS.seek 7ml @22:38:11.41 CSV.dial CTV @22:38:11.45
CTV.dial CSV @22:38:12.96 CBV.dial :waste1 @22:38:13.80 CS.seek 0ml @22:38:31.41 Flushing lower manifold's waste2b port @22:38:31.54 CSV.dial :flush @22:38:32.38 CS.seek 5ml @22:38:45.23 delay 5 seconds @22:38:50.26 CSV.dial :air @22:38:50.83 CS.seek 7ml @22:38:56.60 CSV.dial CTV @22:38:56.64
CTV.dial CSV @22:38:57.12 CBV.dial :waste2b @22:38:57.97 CS.seek 0ml @22:39:15.51 Flushing upper manifold @22:39:15.64 CSV.dial :flush @22:39:17.16 CS.seek 5ml @22:39:29.98 delay 5 seconds @22:39:35.01 CSV.dial :air @22:39:35.58 CS.seek 7ml @22:39:41.29 CSV.dial CBV @22:39:41.32
CBV.dial CSV @22:39:42.51 CTV.dial :air @22:39:43.99 CS.seek 0ml @22:40:01.57 delay 7 seconds @22:40:08.61 CS.seek 3ml @22:40:16.79 CSV.dial :waste @22:40:17.28 CS.seek 0ml @22:40:25.47 CSV.dial :flush @22:40:25.96 CS.seek 3ml @22:40:34.10 delay 5 seconds @22:40:39.13 CSV.dial :air @22:40:39.69 CS.seek 5ml @22:40:45.38 CSV.dial :air @22:40:45.40 CS.seek 0ml @22:40:58.23 delay 7 seconds @22:41:05.27 CS.seek 3ml @22:41:13.45 CSV.dial :waste @22:41:14.27 CS.seek 0ml @22:41:22.48 Disinfecting external Intake with mfbkill @22:41:22.61 CSV.dial CSR @22:41:24.09 Pulling 8ml of CSR.mfbkill @22:41:24.10 CSR.select :mfbkill @22:41:24.23 CS.seek 8ml @22:41:44.14 delay 5 seconds @22:41:49.17 CSR.select :air @22:41:49.30 CS.seek 10ml @22:41:55.07 CSV.dial CBV @22:41:55.11
CBV.dial CSV @22:41:56.23 CTV.dial :intake @22:41:57.06 Intake.select :open @22:41:57.18 CS.seek 2ml @22:42:18.32 Intake.select :close @22:42:18.43 CTV.dial :air @22:42:19.26 CS.reconfigure CSconfig @22:42:19.31 CS.seek 0ml @22:42:25.42 Flushing lower manifold's waste1 port @22:42:25.43 CSV.dial CSR @22:42:26.62 Pulling 4ml of CSR.mfbkill @22:42:26.63 CSR.select :mfbkill @22:42:26.76 CS.seek 4ml @22:42:37.95 delay 5 seconds @22:42:42.98 CSR.select :air @22:42:43.11 CS.reconfigure CSfast @22:42:43.15 CS.seek 10ml @22:42:58.42 CSV.dial CTV @22:42:58.46
CTV.dial CSV @22:42:59.98 CBV.dial :waste1 @22:43:00.47 CS.seek 0ml @22:43:25.23 Flushing lower manifold's waste2b port @22:43:25.24 CSV.dial CSR @22:43:25.79 Pulling 4ml of CSR.mfbkill @22:43:25.80 CSR.select :mfbkill @22:43:25.93 CS.reconfigure CSconfig @22:43:25.97 CS.seek 4ml @22:43:37.20 delay 5 seconds @22:43:42.23 CSR.select :air @22:43:42.35 CS.reconfigure CSfast @22:43:42.40 CS.seek 10ml @22:43:57.54 CSV.dial CTV @22:43:57.57
CTV.dial CSV @22:43:58.08 CBV.dial :waste2b @22:43:58.93 CS.seek 0ml @22:44:23.42 Killing upper manifold @22:44:23.43 CSV.dial CSR @22:44:23.98 Pulling 4ml of CSR.mfbkill @22:44:23.99 CSR.select :mfbkill @22:44:24.11 CS.reconfigure CSconfig @22:44:24.16 CS.seek 4ml @22:44:35.39 delay 5 seconds @22:44:40.42 CSR.select :air @22:44:40.54 CS.reconfigure CSfast @22:44:40.58 CS.seek 10ml @22:44:56.48 CSV.dial CBV @22:44:56.52
CBV.dial CSV @22:44:57.67 CTV.dial :air @22:44:59.13 CS.seek 0ml @22:45:23.83 delay 7 seconds @22:45:30.87 CS.seek 3ml @22:45:38.92 CSV.dial :waste @22:45:39.40 CS.seek 0ml @22:45:47.73 Flushing upper manifold @22:45:47.87 CSV.dial :flush @22:45:48.35 CS.seek 5ml @22:46:01.22 delay 5 seconds @22:46:06.26 CSV.dial :air @22:46:06.82 CS.seek 7ml @22:46:12.52 CSV.dial CBV @22:46:12.56
CBV.dial CSV @22:46:13.69 CTV.dial :air @22:46:13.71 CS.seek 0ml @22:46:31.55 delay 7 seconds @22:46:38.59 CS.seek 3ml @22:46:46.70 CSV.dial :waste @22:46:47.20 CS.seek 0ml @22:46:55.61 CSV.dial :flush @22:46:56.10 CS.seek 3ml @22:47:04.20 delay 5 seconds @22:47:09.23 CSV.dial :air @22:47:09.91 CS.seek 5ml @22:47:15.89 CSV.dial :air @22:47:15.91 CS.seek 0ml @22:47:28.74 delay 7 seconds @22:47:35.78 CS.seek 3ml @22:47:43.96 CSV.dial :waste @22:47:44.76 CS.seek 0ml @22:47:52.83 Flushing lower manifold's waste1 port @22:47:52.96 CSV.dial :flush @22:47:53.47 CS.seek 5ml @22:48:06.24 delay 5 seconds @22:48:11.27 CSV.dial :air @22:48:11.82 CS.seek 7ml @22:48:17.52 CSV.dial CTV @22:48:17.56
CTV.dial CSV @22:48:19.08 CBV.dial :waste1 @22:48:19.59 CS.seek 0ml @22:48:37.46 Flushing lower manifold's waste2b port @22:48:37.59 CSV.dial :flush @22:48:38.42 CS.seek 5ml @22:48:51.18 delay 5 seconds @22:48:56.21 CSV.dial :air @22:48:56.78 CS.seek 7ml @22:49:02.61 CSV.dial CTV @22:49:02.65
CTV.dial CSV @22:49:03.13 CBV.dial :waste2b @22:49:03.98 CS.seek 0ml @22:49:21.46 Disinfecting external Intake with kill @22:49:21.59 CSV.dial CSR @22:49:22.13 Pulling 8ml of CSR.kill @22:49:22.14 CSR.select :kill @22:49:22.27 CS.seek 8ml @22:49:42.21 delay 5 seconds @22:49:47.24 CSR.select :air @22:49:47.37 CS.seek 10ml @22:49:53.08 CSV.dial CBV @22:49:53.12
CBV.dial CSV @22:49:54.29 CTV.dial :intake @22:49:55.10 Intake.select :open @22:49:55.22 CS.seek 2ml @22:50:16.52 Intake.select :close @22:50:16.63 CTV.dial :air @22:50:17.44 CS.reconfigure CSconfig @22:50:18.18 CS.seek 0ml @22:50:24.65 CSV.dial CSR @22:50:25.83 Pulling 5ml of CSR.flush @22:50:25.84 CSR.select :flush @22:50:25.97 CS.seek 5ml @22:50:39.80 delay 5 seconds @22:50:44.83 CSR.select :air @22:50:44.96 CS.seek 10ml @22:50:58.96 CSV.dial CBV @22:51:00.06 CS.seek 0ml @22:51:26.58 Garaging FlushPuck in Collection Clamp @22:51:26.66 Elbow.reconfigure TightElbow @22:51:26.71 Elbow.seek CC @22:51:26.97 Elbow.reconfigure LooseElbow @22:51:27.08 Forearm.seek CC @22:51:28.05 Hand.close @22:51:28.66 CC.seek :open @22:51:33.06 Forearm.seek :retract @22:51:34.07 Elbow.reconfigure TightElbow @22:51:34.12 Elbow.seek :garage @22:51:37.30 Elbow.reconfigure LooseElbow @22:51:37.41 Forearm.reconfigure TightForearm @22:51:37.46 Forearm.seek :garage @22:51:38.61 Hand.open @22:51:39.48 Forearm.reconfigure LooseForearm @22:51:39.53 Forearm.seek :retract @22:51:40.52 Starting initialPurge @22:51:40.53 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @22:51:40.61 Hand.open @22:51:40.98 Elbow.reconfigure TightElbow @22:51:41.02 Elbow.seek FlushPuck::Garage @22:51:41.16 Elbow.reconfigure LooseElbow @22:51:41.27 Forearm.seek :garage @22:51:42.29 Hand.close @22:51:43.04 Forearm.seek :retract @22:51:44.08 Elbow.reconfigure TightElbow @22:51:44.13 Elbow.seek CC @22:51:47.05 Elbow.reconfigure LooseElbow @22:51:47.15 Forearm.reconfigure TightForearm @22:51:47.20 Forearm.seek CC @22:51:48.55 CC.seek :closed @22:51:52.83 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.09 @22:51:52.90 Hand.open @22:51:53.61 Forearm.reconfigure LooseForearm @22:51:53.65 Forearm.seek :retract @22:51:54.66 Flushing the Collection Valve::Manifold @22:51:54.79 CSV.dial CSR @22:51:55.97 Pulling 5ml of CSR.flush @22:51:55.98 CSR.select :flush @22:51:56.10 CS.seek 5ml @22:52:09.84 delay 5 seconds @22:52:14.87 CSR.select :air @22:52:15.04 CS.reconfigure CSfast @22:52:15.09 CS.seek 7.5ml @22:52:22.09 CSV.dial :waste @22:52:23.51 CS.reconfigure CSconfig @22:52:23.56 CS.seek 0ml @22:52:43.68 Purging external Intake @22:52:44.50 CSV.dial :flush @22:52:44.99 CS.reconfigure CSfast @22:52:45.04 CS.seek 10ml @22:53:09.59 delay 5 seconds @22:53:14.62 CSV.dial CBV @22:53:14.66
CBV.dial CSV @22:53:15.49 CTV.dial :intake @22:53:16.31 Intake.select :open @22:53:16.43 CS.seek 0ml @22:53:42.46 Intake.select :close @22:53:42.57 CTV.dial :air @22:53:43.38 Flushing upper manifold @22:53:43.51 CSV.dial :flush @22:53:44.33 CS.seek 5ml @22:53:57.03 delay 5 seconds @22:54:02.06 CSV.dial :air @22:54:02.61 CS.seek 7ml @22:54:08.31 CSV.dial CBV @22:54:08.33
CBV.dial CSV @22:54:09.48 CTV.dial :air @22:54:09.50 CS.seek 0ml @22:54:26.99 delay 7 seconds @22:54:34.03 CS.seek 3ml @22:54:42.18 CSV.dial :waste @22:54:42.68 CS.seek 0ml @22:54:50.99 CSV.dial :flush @22:54:51.49 CS.seek 3ml @22:54:59.62 delay 5 seconds @22:55:04.65 CSV.dial :air @22:55:05.21 CS.seek 5ml @22:55:10.93 CSV.dial :air @22:55:10.95 CS.seek 0ml @22:55:23.75 delay 7 seconds @22:55:30.79 CS.seek 3ml @22:55:38.90 CSV.dial :waste @22:55:39.71 CS.seek 0ml @22:55:47.78 Garaging FlushPuck in Collection Clamp @22:55:47.86 Elbow.reconfigure TightElbow @22:55:47.91 Elbow.seek CC @22:55:48.17 Elbow.reconfigure LooseElbow @22:55:48.28 Forearm.seek CC @22:55:49.26 Hand.close @22:55:49.92 CC.seek :open @22:55:54.28 Forearm.seek :retract @22:55:55.26 Elbow.reconfigure TightElbow @22:55:55.31 Elbow.seek :garage @22:55:58.48 Elbow.reconfigure LooseElbow @22:55:58.58 Forearm.reconfigure TightForearm @22:55:58.63 Forearm.seek :garage @22:55:59.78 Hand.open @22:56:00.49 Forearm.reconfigure LooseForearm @22:56:00.53 Forearm.seek :retract @22:56:01.53 Completed [wcrphase] begun 22:00:27 @22:56:01.54 Preparing to sleep until 04:00 28-may-20 @22:56:01.61 SS.coast @22:56:01.63
Asleep til 04:00 28-may-20 @22:56:01.71 Continuing at 04:00CEST28-May-20 @22:56:01.74 CC.coast @22:56:01.84 CS.coast @22:56:01.87 GoodNews.email "Then, in tube 4: wcrphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:167\n{1 => 22, 2 => 22, 3 => 2, 4 => 20}\nCan@22:56:01, 9.5C, 50% humidity, 14.4psia, 12.969V, 0.525A, 0.525A avg, 6.81W",Subject: "Asleep until 04:00CEST28-May-20" @22:56:01.95 PC.coast @22:56:01.99 Forearm.coast @22:56:02.03 Elbow.coast @22:56:02.43 Gate.power :core,:OFF @22:56:02.45 Gate.power :raw,:OFF @22:56:02.46 Shutdown @04:00:00.06CEST28-May-20
Powering core bus @04:00:04.90 Gate.power :raw,:ON @04:00:05.56 SPE.coast @04:00:05.62 SPE.configure SPEconfig @04:00:05.72 AS.configure ASconfig @04:00:05.82 SS.configure SSconfig @04:00:05.92 CC.configure CCconfig @04:00:06.01 CS.configure CSconfig @04:00:06.12 PC.configure PCconfig @04:00:06.21 PS.configure PSconfig @04:00:06.33 Forearm.configure LooseForearm @04:00:06.43 Elbow.configure LooseElbow @04:00:06.55 SC.configure SCloose @04:00:06.65 SE.configure SEconfig @04:00:06.69 CH.coast @04:00:06.75 CH.configure CHconfig @04:00:06.78 PH.coast @04:00:06.84 PH.configure PHconfig @04:00:06.88 PCR.off @04:00:06.91 PCR.pidtemp= 50,0.1,0,300,25,25 @04:00:06.94 PCR.caltemp= 2.8814,0.0372,0 @04:00:06.97 All dwarf controllers running firmware version 3.75 @04:00:07.08 Valve.reset @04:00:07.25 PCR.off @04:00:07.29 PCR.pidtemp= 50,0.1,0,300,25,25 @04:00:07.32 PCR.caltemp= 2.8814,0.0372,0 @04:00:07.54 PSV.dial :air @04:00:07.69 Intake.select :close @04:00:07.72 PS.reconfigure PSconfig @04:00:07.73 Exhaust.select :close @04:00:07.76 CTV.dial :air @04:00:07.83 PS.home.seek 0ml @04:00:07.93 CSV.dial :waste1 @04:00:07.95
Forearm.reconfigure LooseForearm @04:00:08.05 Forearm.home.seek :retract @04:00:08.11 SSV.dial :spit,avoiding: :exhaust @04:00:08.80 Hand.close @04:00:08.87
Elbow.jog 1500 @04:00:08.99 CS.reconfigure CSfast @04:00:09.02 SS.reconfigure SSconfig @04:00:09.14 CS.home.seek 0ml @04:00:09.16 SS.home.seek 0ml @04:00:09.55
Elbow.reconfigure LooseElbow @04:00:09.62 Elbow.home.seek :clear @04:00:13.08 SE.reconfigure SEconfig @04:00:13.12 SE.home.seek :down @04:00:13.75 SC.reconfigure SCsloppy @04:00:13.80 SC.home.seek :initial @04:00:16.89 PS.coast @04:00:19.39
Forearm.seek :retract @04:00:19.47 Elbow.seek :hammer @04:00:20.74 Forearm.reconfigure TightForearm @04:00:20.79 Forearm.seek 5 + 350 counts @04:00:21.86 Forearm.seek :retract @04:00:23.09 PC.reconfigure PCconfig @04:00:23.11
CC.reconfigure CCconfig @04:00:23.20 PC.home.seek :open @04:00:23.23
CC.home.seek :open @04:00:26.91 Phase [wcrphase] @04:00:26.92 GoodNews.email "At 04:00:26 began in tube 4: wcrphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:167\n{1 => 22, 2 => 22, 3 => 2, 4 => 20}\nCan@04:00:26, 8.7C, 51% humidity, 14.3psia, 12.999V, 0.645A, 0.645A avg, 8.38W",Subject: "Started 20invasivedeployfinal phase wcrphase" @04:00:26.94CEST28-May-20
===> STARTED WCRSphase (phase #20) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @04:00:26.98 Starting initialPurge @04:00:26.99 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @04:00:27.07 Hand.open @04:00:27.99 Elbow.reconfigure TightElbow @04:00:28.05 Elbow.seek FlushPuck::Garage @04:00:30.01 Elbow.reconfigure LooseElbow @04:00:30.12 Forearm.reconfigure LooseForearm @04:00:30.19 Forearm.seek :garage @04:00:31.23 Hand.close @04:00:32.13 Forearm.seek :retract @04:00:33.16 Elbow.reconfigure TightElbow @04:00:33.23 Elbow.seek CC @04:00:36.35 Elbow.reconfigure LooseElbow @04:00:36.61 Forearm.reconfigure TightForearm @04:00:36.74 Forearm.seek CC @04:00:39.17 CC.seek :closed @04:00:43.64 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.27 @04:00:43.81 Hand.open @04:00:44.75 Forearm.reconfigure LooseForearm @04:00:44.84 Forearm.seek :retract @04:00:45.89 Flushing the Collection Valve::Manifold @04:00:46.16 CSV.dial CSR @04:00:47.79 Pulling 5ml of CSR.flush @04:00:47.80 CSR.select :flush @04:00:47.97 CS.reconfigure CSconfig @04:00:48.07 CS.seek 5ml @04:01:01.84 delay 5 seconds @04:01:06.88 CSR.select :air @04:01:07.15 CS.reconfigure CSfast @04:01:07.25 CS.seek 7.5ml @04:01:14.27 CSV.dial :waste @04:01:15.74 CS.reconfigure CSconfig @04:01:15.88 CS.seek 0ml @04:01:36.07 Purging external Intake @04:01:36.20 CSV.dial :flush @04:01:36.70 CS.reconfigure CSfast @04:01:36.75 CS.seek 10ml @04:02:01.41 delay 5 seconds @04:02:06.44 CSV.dial CBV @04:02:06.48
CBV.dial CSV @04:02:07.32 CTV.dial :intake @04:02:08.16 Intake.select :open @04:02:08.28 CS.seek 0ml @04:02:34.72 Intake.select :close @04:02:34.83 CTV.dial :air @04:02:35.66 Flushing upper manifold @04:02:35.79 CSV.dial :flush @04:02:36.61 CS.seek 5ml @04:02:49.44 delay 5 seconds @04:02:54.47 CSV.dial :air @04:02:55.83 CS.seek 7.001ml @04:03:01.66 CSV.dial CBV @04:03:01.70
CBV.dial CSV @04:03:02.93 CTV.dial :air @04:03:02.95 CS.seek 0ml @04:03:20.51 delay 7 seconds @04:03:27.55 CS.seek 3ml @04:03:35.66 CSV.dial :waste @04:03:36.14 CS.seek 0ml @04:03:44.44 CSV.dial :flush @04:03:44.92 CS.seek 3ml @04:03:53.07 delay 5 seconds @04:03:58.10 CSV.dial :air @04:03:58.66 CS.seek 5ml @04:04:04.35 CSV.dial :air @04:04:04.37 CS.seek 0ml @04:04:17.10 delay 7 seconds @04:04:24.14 CS.seek 3ml @04:04:32.35 CSV.dial :waste @04:04:33.16 CS.seek 0ml @04:04:41.23 Garaging FlushPuck in Collection Clamp @04:04:41.31 Elbow.reconfigure TightElbow @04:04:41.36 Elbow.seek CC @04:04:41.60 Elbow.reconfigure LooseElbow @04:04:41.71 Forearm.seek CC @04:04:42.70 Hand.close @04:04:43.47 CC.seek :open @04:04:47.86 Forearm.seek :retract @04:04:48.86 Elbow.reconfigure TightElbow @04:04:48.91 Elbow.seek :garage @04:04:52.04 Elbow.reconfigure LooseElbow @04:04:52.14 Forearm.reconfigure TightForearm @04:04:52.19 Forearm.seek :garage @04:04:53.34 Hand.open @04:04:54.16 Forearm.reconfigure LooseForearm @04:04:54.20 Forearm.seek :retract @04:04:55.22 Starting stand-alone bac WCR @04:04:55.23 bac wcr sampling at most 1500ml @04:04:55.26 Whole Cell Archive sampling 1500ml @04:04:55.28 BACfans.email "Started stand-alone bac WCR in tube 4 at 04:04:55CEST28-May-20\n{1 => 22, 2 => 22, 3 => 2, 4 => 20}\nCan@04:00:26, 8.7C, 51% humidity, 14.3psia, 12.999V, 0.645A, 0.645A avg, 8.38W",Subject: "bac wcr sampling at most 1500ml" @04:04:55.30 Loading wcr puck from tube 4 into Collection Clamp @04:04:55.58 SC.reconfigure SCtight @04:04:55.64 SC.seek 4 @04:04:59.27 SC.reconfigure SCloose @04:04:59.52 Elbow.reconfigure TightElbow @04:04:59.57 Elbow.seek 4 @04:05:01.69 Forearm.reconfigure TightForearm @04:05:01.74 Forearm.seek 4 + 700 counts @04:05:02.89 SE.reconfigure SEpick @04:05:03.06 SE.seek beamBreak @04:05:06.13 SE.configure SEconfig @04:05:06.59 Elbow.seek 4 @04:05:07.08 Forearm.seek 4 + 300 counts @04:05:07.35 Forearm.reconfigure LooseForearm @04:05:07.45 Hand.close @04:05:08.15 SE.seek :down @04:05:08.76 Forearm.seek :retract @04:05:09.26 SE.coast @04:05:09.83 Elbow.seek CC @04:05:11.69 Elbow.reconfigure LooseElbow @04:05:11.91 Forearm.reconfigure TightForearm @04:05:12.07 Forearm.seek CC @04:05:13.87 CC.seek :closed @04:05:18.38 Collection Clamp onSpring/offSpring for 6/30 samples, on/off torque 2.26 @04:05:18.42 Hand.open @04:05:19.25 Forearm.reconfigure LooseForearm @04:05:19.36 Forearm.seek :retract @04:05:20.46 SC.coast @04:05:20.55 collectSample 1500ml w/bubble point of 55psi @04:05:20.58 Priming 7ml @04:05:20.60 Intake.select :close @04:05:20.62 Exhaust.select :close @04:05:20.65 SS.hold @04:05:21.14 Calibrating pressure and temperature sensors @04:05:21.18 CTV.dial :air @04:05:21.20 SSV.dial :spit,avoiding: :exhaust @04:05:21.24 SS.reconfigure SSfast @04:05:21.39 SS.seek 5ml @04:05:24.31 SSV.dial CBV @04:05:24.42 CBV.dial SSV @04:05:25.98 delay 3.8 seconds @04:05:30.06 SSV.dial :spit @04:05:31.61 SS.seek 0ml @04:05:34.16 SSV.dial CBV @04:05:35.64 CTV.dial :intake @04:05:36.56 SSV.dial CBV @04:05:36.63 CBV.dial SSV @04:05:36.66 Allowing 40s for 7.0ml of remaining 7.0ml @04:05:36.68 Intake.select :open @04:05:38.96 Intake @31.6psia, vacuum from 20.0 to 24.0psi @04:05:39.00 SS.reconfigure SSinhale.with maxSpeed: 150 @04:05:39.13 SS.seek 7ml @04:05:43.20 Vacuum too high -- waiting up to 40s @04:05:48.35 SS.reconfigure SSinhale.with maxSpeed: 135 @04:05:48.45 SS.seek 7ml @04:05:51.78 Stroke completed -- waiting for pressure to equalize ... @04:05:56.67 Intake.select :close @04:05:56.95 SSV.dial :exhaust @04:05:57.83 Exhaust.select :open @04:05:58.00 SS.reconfigure SSexhale @04:05:58.10 SS.seek 0ml @04:06:01.75 Exhaust.select :close @04:06:01.91 Sampling 1500.0ml @04:06:01.94 Intake.select :close @04:06:01.96 Exhaust.select :close @04:06:01.99 SS.reconfigure SSconfig @04:06:02.07 SS.hold @04:06:02.52 CTV.dial :intake @04:06:02.57 SSV.dial CBV @04:06:02.62 CBV.dial SSV @04:06:03.53 Allowing 130s for 25.0ml of remaining 1500.0ml @04:06:03.57 Intake.select :open @04:06:05.85 Intake @32.0psia, vacuum from 20.0 to 24.0psi @04:06:05.89 SS.reconfigure SSinhale.with maxSpeed: 135 @04:06:05.98 SS.seek 25ml @04:06:10.14 Vacuum too high -- waiting up to 40s @04:06:12.49 Intake @31.5psia, vacuum from 20.0 to 24.0psi @04:06:12.51 SS.reconfigure SSinhale.with maxSpeed: 122 @04:06:12.56 SS.seek 25ml @04:06:15.44 Vacuum too high -- waiting up to 40s @04:06:18.01 Intake @31.5psia, vacuum from 20.0 to 24.0psi @04:06:18.03 SS.reconfigure SSinhale.with maxSpeed: 110 @04:06:18.08 SS.seek 25ml @04:06:21.10 Vacuum too high -- waiting up to 40s @04:06:23.67 Intake @31.5psia, vacuum from 20.0 to 24.0psi @04:06:23.69 SS.reconfigure SSinhale.with maxSpeed: 99 @04:06:23.74 SS.seek 25ml @04:06:26.44 Vacuum too high -- waiting up to 40s @04:06:28.97 Intake @31.6psia, vacuum from 20.0 to 24.0psi @04:06:28.99 SS.reconfigure SSinhale.with maxSpeed: 89 @04:06:29.04 SS.seek 25ml @04:06:31.72 Vacuum too high -- waiting up to 40s @04:06:34.07 SS.reconfigure SSinhale.with maxSpeed: 80 @04:06:34.12 SS.seek 25ml @04:06:36.95 Vacuum too high -- waiting up to 40s @04:06:39.54 SS.reconfigure SSinhale.with maxSpeed: 72 @04:06:39.59 SS.seek 25ml @04:06:42.69 Vacuum too high -- waiting up to 40s @04:06:45.02 Intake @31.6psia, vacuum from 20.0 to 24.0psi @04:06:45.04 SS.reconfigure SSinhale.with maxSpeed: 65 @04:06:45.09 SS.seek 25ml @04:06:47.86 Vacuum too high -- waiting up to 40s @04:06:50.19 SS.reconfigure SSinhale.with maxSpeed: 59 @04:06:50.24 SS.seek 25ml @04:06:53.27 Vacuum too high -- waiting up to 40s @04:06:55.56 Intake @31.7psia, vacuum from 20.0 to 24.0psi @04:06:55.58 SS.reconfigure SSinhale.with maxSpeed: 53 @04:06:55.64 SS.seek 25ml @04:06:57.16 Stroke completed -- waiting for pressure to equalize ... @04:07:02.26 Intake.select :close @04:07:02.42 SSV.dial :exhaust @04:07:03.25 Exhaust.select :open @04:07:03.37 SS.reconfigure SSexhale @04:07:03.42 SS.seek 0ml @04:07:12.23 Exhaust.select :close @04:07:12.35 SSV.dial CBV @04:07:12.37 CBV.dial SSV @04:07:13.23 Allowing 130s for 25.0ml of remaining 1475.0ml @04:07:13.24 Intake.select :open @04:07:15.42 Intake @32.0psia, vacuum from 20.0 to 24.0psi @04:07:15.44 SS.reconfigure SSinhale.with maxSpeed: 53 @04:07:15.49 SS.seek 25ml @04:07:21.31 Vacuum too high -- waiting up to 40s @04:07:23.38 Intake @31.7psia, vacuum from 20.0 to 24.0psi @04:07:23.40 SS.reconfigure SSinhale.with maxSpeed: 48 @04:07:23.45 SS.seek 25ml @04:07:27.94 Vacuum too high -- waiting up to 40s @04:07:30.02 SS.reconfigure SSinhale.with maxSpeed: 43 @04:07:30.08 SS.seek 25ml @04:07:35.06 Vacuum too high -- waiting up to 40s @04:07:37.38 Intake @31.6psia, vacuum from 20.0 to 24.0psi @04:07:37.40 SS.reconfigure SSinhale.with maxSpeed: 39 @04:07:37.45 SS.seek 25ml @04:07:44.12 Vacuum too high -- waiting up to 40s @04:07:46.40 Intake @31.7psia, vacuum from 20.0 to 24.0psi @04:07:46.42 SS.reconfigure SSinhale.with maxSpeed: 35 @04:07:46.47 SS.seek 25ml @04:07:54.16 Vacuum too high -- waiting up to 40s @04:07:56.52 Intake @31.7psia, vacuum from 20.0 to 24.0psi @04:07:56.54 SS.reconfigure SSinhale.with maxSpeed: 32 @04:07:56.59 SS.seek 25ml @04:08:06.93 Vacuum too high -- waiting up to 40s @04:08:09.26 Intake @31.7psia, vacuum from 20.0 to 24.0psi @04:08:09.28 SS.reconfigure SSinhale.with maxSpeed: 29 @04:08:09.33 SS.seek 25ml @04:08:20.05 Stroke completed -- waiting for pressure to equalize ... @04:08:27.68 Intake.select :close @04:08:27.84 SSV.dial :exhaust @04:08:28.68 Exhaust.select :open @04:08:28.80 SS.reconfigure SSexhale @04:08:28.84 SS.seek 0ml @04:08:37.98 Exhaust.select :close @04:08:38.10 SSV.dial CBV @04:08:38.12 CBV.dial SSV @04:08:38.98 Allowing 130s for 25.0ml of remaining 1450.0ml @04:08:38.99 Intake.select :open @04:08:41.16 Intake @31.9psia, vacuum from 20.0 to 24.0psi @04:08:41.18 SS.reconfigure SSinhale.with maxSpeed: 29 @04:08:41.23 SS.seek 25ml @04:09:00.96 Vacuum too high -- waiting up to 40s @04:09:03.99 Intake @31.6psia, vacuum from 20.0 to 24.0psi @04:09:04.01 SS.reconfigure SSinhale.with maxSpeed: 26 @04:09:04.06 SS.seek 25ml @04:09:37.88 Vacuum too high -- waiting up to 40s @04:09:40.48 Intake @31.7psia, vacuum from 20.0 to 24.0psi @04:09:40.50 SS.reconfigure SSinhale.with maxSpeed: 23 @04:09:40.55 SS.seek 25ml @04:09:54.66 Stroke completed -- waiting for pressure to equalize ... @04:10:02.24 Intake.select :close @04:10:02.40 SSV.dial :exhaust @04:10:03.23 Exhaust.select :open @04:10:03.35 SS.reconfigure SSexhale @04:10:03.40 SS.seek 0ml @04:10:12.37 Exhaust.select :close @04:10:12.49 SSV.dial CBV @04:10:12.51 CBV.dial SSV @04:10:13.37 Allowing 130s for 25.0ml of remaining 1425.0ml @04:10:13.38 Intake.select :open @04:10:15.57 Intake @32.0psia, vacuum from 20.0 to 24.0psi @04:10:15.59 SS.reconfigure SSinhale.with maxSpeed: 23 @04:10:15.64 SS.seek 25ml @04:11:32.00 Stroke completed -- waiting for pressure to equalize ... @04:11:41.40 Intake.select :close @04:11:41.56 SSV.dial :exhaust @04:11:42.40 Exhaust.select :open @04:11:42.52 SS.reconfigure SSexhale @04:11:42.58 SS.seek 0ml @04:11:51.51 Exhaust.select :close @04:11:51.63 SSV.dial CBV @04:11:51.65 CBV.dial SSV @04:11:52.51 Allowing 130s for 25.0ml of remaining 1400.0ml @04:11:52.52 Intake.select :open @04:11:55.40 Intake @32.0psia, vacuum from 20.0 to 24.0psi @04:11:55.42 SS.reconfigure SSinhale.with maxSpeed: 23 @04:11:55.46 SS.seek 25ml @04:12:52.72 Vacuum too high -- waiting up to 40s @04:12:55.33 Intake @31.5psia, vacuum from 20.0 to 24.0psi @04:12:55.35 SS.reconfigure SSinhale.with maxSpeed: 21 @04:12:55.40 SS.seek 25ml @04:13:16.94 Stroke completed -- waiting for pressure to equalize ... @04:13:26.37 Intake.select :close @04:13:26.53 SSV.dial :exhaust @04:13:27.38 Exhaust.select :open @04:13:27.50 SS.reconfigure SSexhale @04:13:27.55 SS.seek 0ml @04:13:36.80 Exhaust.select :close @04:13:36.92 SSV.dial CBV @04:13:36.94 CBV.dial SSV @04:13:37.80 Allowing 130s for 25.0ml of remaining 1375.0ml @04:13:37.81 Intake.select :open @04:13:39.99 Intake @32.0psia, vacuum from 20.0 to 24.0psi @04:13:40.01 SS.reconfigure SSinhale.with maxSpeed: 21 @04:13:40.06 SS.seek 25ml @04:14:55.69 Vacuum too high -- waiting up to 40s @04:14:58.76 Intake @31.6psia, vacuum from 20.0 to 24.0psi @04:14:58.78 SS.reconfigure SSinhale.with maxSpeed: 19 @04:14:58.83 SS.seek 25ml @04:15:07.85 Stroke completed -- waiting for pressure to equalize ... @04:15:17.49 Intake.select :close @04:15:17.65 SSV.dial :exhaust @04:15:18.50 Exhaust.select :open @04:15:18.62 SS.reconfigure SSexhale @04:15:18.67 SS.seek 0ml @04:15:27.98 Exhaust.select :close @04:15:28.10 SSV.dial CBV @04:15:28.12 CBV.dial SSV @04:15:28.98 Allowing 130s for 25.0ml of remaining 1350.0ml @04:15:28.99 Intake.select :open @04:15:31.17 Intake @32.0psia, vacuum from 20.0 to 24.0psi @04:15:31.19 SS.reconfigure SSinhale.with maxSpeed: 19 @04:15:31.24 SS.seek 25ml @04:17:01.53 Vacuum too high -- waiting up to 40s @04:17:04.61 Intake @31.5psia, vacuum from 20.0 to 24.0psi @04:17:04.63 SS.reconfigure SSinhale.with maxSpeed: 17 @04:17:04.68 SS.seek 25ml @04:17:07.48 Stroke completed -- waiting for pressure to equalize ... @04:17:16.28 Intake.select :close @04:17:16.44 SSV.dial :exhaust @04:17:17.28 Exhaust.select :open @04:17:17.40 SS.reconfigure SSexhale @04:17:17.45 SS.seek 0ml @04:17:26.59 Exhaust.select :close @04:17:26.71 SSV.dial CBV @04:17:26.73 CBV.dial SSV @04:17:27.59 Allowing 130s for 25.0ml of remaining 1325.0ml @04:17:27.60 Intake.select :open @04:17:29.77 Intake @31.9psia, vacuum from 20.0 to 24.0psi @04:17:29.79 SS.reconfigure SSinhale.with maxSpeed: 17 @04:17:29.84 SS.seek 25ml @04:19:12.85 Stroke completed -- waiting for pressure to equalize ... @04:19:27.43 Intake.select :close @04:19:27.59 SSV.dial :exhaust @04:19:28.43 Exhaust.select :open @04:19:28.55 SS.reconfigure SSexhale @04:19:28.60 SS.seek 0ml @04:19:37.95 Exhaust.select :close @04:19:38.07 SSV.dial CBV @04:19:38.09 CBV.dial SSV @04:19:38.95 Allowing 130s for 25.0ml of remaining 1300.0ml @04:19:38.96 Intake.select :open @04:19:41.14 Intake @31.9psia, vacuum from 20.0 to 24.0psi @04:19:41.16 SS.reconfigure SSinhale.with maxSpeed: 17 @04:19:41.21 SS.seek 25ml @04:20:33.57 Vacuum too high -- waiting up to 40s @04:20:37.14 Intake @31.6psia, vacuum from 20.0 to 24.0psi @04:20:37.16 SS.reconfigure SSinhale.with maxSpeed: 15 @04:20:37.20 SS.seek 25ml @04:21:34.97 Stroke completed -- waiting for pressure to equalize ... @04:21:48.97 SS.reconfigure SSconfig @04:21:49.02 SS.hold @04:21:49.15 Intake.select :close @04:21:49.31 ShallowSampler::Clogged in WCR -- progress too slow after 225.0ml @04:21:49.33 SSV.dial :exhaust @04:21:50.16 Exhaust.select :open @04:21:50.28 SS.reconfigure SSexhale @04:21:50.33 SS.seek 0ml @04:21:59.66 Exhaust.select :close @04:21:59.77 Sampled 225.0ml @04:21:59.79 Drying puck before fixing for 4:00 @04:21:59.80 CBV.dial :waste2b @04:22:00.64 delay 1:00 @04:23:00.68 CSV.dial :air @04:23:01.49 CS.seek 10ml @04:23:26.06 Pumping wcr puck from tube 4 up to 55psi... @04:23:26.08 CBV.dial SSV @04:23:27.48 CBV.dial between CSV and waste2b @04:23:28.55 CSV.dial between air and CTV @04:23:28.89 CS.seek 7.091ml @04:23:36.85 CTV.dial CSV,avoiding: :air @04:23:36.87 CSV.dial CTV @04:23:37.75 CS.reconfigure CSpressurize @04:23:37.81 CS.seek 0ml @04:23:56.56 CSV.dial :air @04:23:57.05 CS.reconfigure CSfast @04:23:57.11 CS.seek 10ml @04:24:21.81 CSV.dial between air and CTV @04:24:22.18 CS.seek 2.536ml @04:24:40.92 CTV.dial CSV,avoiding: :air @04:24:40.94 CSV.dial CTV @04:24:41.29 CS.reconfigure CSpressurize @04:24:41.34 CS.seek 0ml @04:24:45.90 CBV.dial :waste2b @04:24:46.28 CS.reconfigure CSconfig @04:24:46.33 CS.seek :empty @04:24:50.00 delay 1:00 @04:25:50.03 CTV.dial :air,avoiding: :intake @04:25:51.49 Fix #1 of 2 (with rnal) for 2:00 @04:25:51.51 CSV.dial CSR @04:25:52.05 Pulling 2ml of CSR.rnal @04:25:52.06 CSR.select :rnal @04:25:52.19 CS.seek 2ml @04:25:58.41 delay 5 seconds @04:26:03.44 CSR.select :air @04:26:03.56 CS.reconfigure CSfast @04:26:03.60 CS.seek 10ml @04:26:23.53 Pumping wcr puck from tube 4 up to 55psi... @04:26:23.54 CBV.dial SSV @04:26:24.92 CBV.dial between CSV and waste2b @04:26:26.68 CSV.dial between air and CTV @04:26:27.36 CS.seek 7.194ml @04:26:35.01 CTV.dial CSV,avoiding: :air @04:26:35.04 CSV.dial CTV @04:26:36.56 CS.reconfigure CSpressurize @04:26:36.61 CS.seek 0ml @04:26:55.60 CSV.dial :air @04:26:56.11 CS.reconfigure CSfast @04:26:56.16 CS.seek 10ml @04:27:20.94 CSV.dial between air and CTV @04:27:21.30 CS.seek 2.164ml @04:27:41.02 CTV.dial CSV,avoiding: :air @04:27:41.04 CSV.dial CTV @04:27:41.39 CS.reconfigure CSpressurize @04:27:41.44 CS.seek 0ml @04:27:43.43 delay 2:00 @04:29:43.46 CBV.dial :waste2b @04:29:43.82 CS.reconfigure CSconfig @04:29:43.87 CS.seek :empty @04:29:48.92 delay 1:00 @04:30:48.96 CTV.dial :air,avoiding: :intake @04:30:50.43 Fix #2 of 2 (with rnal) for 2:00 @04:30:50.45 CSV.dial CSR @04:30:50.99 Pulling 2ml of CSR.rnal @04:30:51.01 CSR.select :rnal @04:30:51.13 CS.seek 2ml @04:30:57.32 delay 5 seconds @04:31:02.35 CSR.select :air @04:31:02.48 CS.reconfigure CSfast @04:31:02.53 CS.seek 10ml @04:31:22.38 Pumping wcr puck from tube 4 up to 55psi... @04:31:22.39 CBV.dial SSV @04:31:23.77 CBV.dial between CSV and waste2b @04:31:24.84 CSV.dial between air and CTV @04:31:25.52 CS.seek 7.225ml @04:31:33.08 CTV.dial CSV,avoiding: :air @04:31:33.10 CSV.dial CTV @04:31:34.65 CS.reconfigure CSpressurize @04:31:34.70 CS.seek 0ml @04:31:53.76 CSV.dial :air @04:31:54.26 CS.reconfigure CSfast @04:31:54.31 CS.seek 10ml @04:32:18.82 CSV.dial between air and CTV @04:32:19.18 CS.seek 2.291ml @04:32:38.55 CTV.dial CSV,avoiding: :air @04:32:38.57 CSV.dial CTV @04:32:38.91 CS.reconfigure CSpressurize @04:32:38.96 CS.seek 0ml @04:32:41.69 delay 2:00 @04:34:41.72 CBV.dial :waste2b @04:34:42.75 CS.reconfigure CSconfig @04:34:42.80 CS.seek :empty @04:34:47.43 delay 1:00 @04:35:47.46 CTV.dial :air,avoiding: :intake @04:35:48.99 Storing wcr puck in Collection Clamp to tube 3 @04:35:49.18 SC.reconfigure SCtight @04:35:49.23 SC.seek 3 @04:35:51.38 SC.reconfigure SCloose @04:35:51.61 Elbow.reconfigure TightElbow @04:35:51.66 Elbow.seek 3 @04:35:53.53 Forearm.reconfigure TightForearm @04:35:53.58 Forearm.seek 3 + 700 counts @04:35:54.65 SE.reconfigure SEpick @04:35:54.74 SE.seek beamBreak @04:35:57.78 SE.configure SEconfig @04:35:57.95 Elbow.reconfigure LimpElbow @04:35:58.00 Forearm.reconfigure LooseForearm @04:35:58.05 Forearm.seek 3 @04:35:58.36 Elbow.hold @04:35:58.45 Elbow.reconfigure TightElbow @04:35:58.55 SE.seek 1.07 Pucks below Top Plate @04:35:59.01 Forearm.force -20 @04:35:59.05 Forearm.seek 3 + 330 counts @04:35:59.38 Forearm.reconfigure TightForearm @04:35:59.43 Forearm.seek 3 + 700 counts @04:35:59.72 SE.seek 2.07 Pucks below Top Plate @04:36:00.33 Forearm.reconfigure LooseForearm @04:36:00.38 Forearm.seek :retract @04:36:01.30 Elbow.seek CC @04:36:03.00 Elbow.reconfigure LooseElbow @04:36:03.11 Forearm.seek CC @04:36:04.13 Hand.close @04:36:04.90 CC.seek :open @04:36:09.43 Forearm.seek :retract @04:36:10.46 Elbow.reconfigure TightElbow @04:36:10.51 Elbow.seek 3 @04:36:12.35 Elbow.reconfigure LimpElbow @04:36:12.40 Forearm.seek 3 @04:36:13.40 Elbow.hold @04:36:13.48 Elbow.reconfigure TightElbow @04:36:13.53 Hand.open @04:36:14.41 Forearm.force -20 @04:36:14.46 Forearm.seek 3 + 346 counts @04:36:15.47 Forearm.reconfigure TightForearm @04:36:15.52 Forearm.seek 3 + 700 counts @04:36:15.88 SE.seek :down @04:36:17.04 Forearm.reconfigure LooseForearm @04:36:17.09 Forearm.seek :retract @04:36:18.49 SE.coast @04:36:18.53 SC.coast @04:36:18.58 Starting finalPurge @04:36:18.59 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @04:36:18.67 Hand.open @04:36:19.04 Elbow.seek FlushPuck::Garage @04:36:21.11 Elbow.reconfigure LooseElbow @04:36:21.21 Forearm.seek :garage @04:36:22.23 Hand.close @04:36:23.03 Forearm.seek :retract @04:36:24.05 Elbow.reconfigure TightElbow @04:36:24.10 Elbow.seek CC @04:36:27.05 Elbow.reconfigure LooseElbow @04:36:27.16 Forearm.reconfigure TightForearm @04:36:27.21 Forearm.seek CC @04:36:28.57 CC.seek :closed @04:36:32.83 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.10 @04:36:32.90 Hand.open @04:36:33.59 Forearm.reconfigure LooseForearm @04:36:33.64 Forearm.seek :retract @04:36:34.66 Flushing the Collection Valve::Manifold @04:36:34.80 CSV.dial CSR @04:36:35.34 Pulling 5ml of CSR.flush @04:36:35.35 CSR.select :flush @04:36:35.47 CS.seek 5ml @04:36:49.30 delay 5 seconds @04:36:54.33 CSR.select :air @04:36:54.49 CS.reconfigure CSfast @04:36:54.54 CS.seek 7.5ml @04:37:01.42 CSV.dial :waste @04:37:02.83 CS.reconfigure CSconfig @04:37:02.88 CS.seek 0ml @04:37:22.95 Flushing lower manifold's waste1 port @04:37:23.08 CSV.dial :flush @04:37:23.56 CS.reconfigure CSfast @04:37:23.62 CS.seek 5ml @04:37:36.52 delay 5 seconds @04:37:41.54 CSV.dial :air @04:37:42.10 CS.seek 7ml @04:37:47.92 CSV.dial CTV @04:37:47.95
CTV.dial CSV @04:37:49.49 CBV.dial :waste1 @04:37:50.34 CS.seek 0ml @04:38:07.92 Flushing lower manifold's waste2b port @04:38:08.75 CSV.dial :flush @04:38:09.57 CS.seek 5ml @04:38:22.39 delay 5 seconds @04:38:27.42 CSV.dial :air @04:38:27.98 CS.seek 7ml @04:38:33.74 CSV.dial CTV @04:38:33.77
CTV.dial CSV @04:38:34.25 CBV.dial :waste2b @04:38:35.11 CS.seek 0ml @04:38:52.71 Flushing upper manifold @04:38:52.85 CSV.dial :flush @04:38:53.67 CS.seek 5ml @04:39:06.52 delay 5 seconds @04:39:11.55 CSV.dial :air @04:39:12.12 CS.seek 7ml @04:39:17.89 CSV.dial CBV @04:39:17.93
CBV.dial CSV @04:39:19.10 CTV.dial :air @04:39:20.58 CS.seek 0ml @04:39:38.11 delay 7 seconds @04:39:45.15 CS.seek 3ml @04:39:53.24 CSV.dial :waste @04:39:53.73 CS.seek 0ml @04:40:01.95 CSV.dial :flush @04:40:02.44 CS.seek 3ml @04:40:10.49 delay 5 seconds @04:40:15.52 CSV.dial :air @04:40:16.07 CS.seek 5ml @04:40:21.77 CSV.dial :air @04:40:21.79 CS.seek 0ml @04:40:34.71 delay 7 seconds @04:40:41.75 CS.seek 3ml @04:40:49.86 CSV.dial :waste @04:40:50.67 CS.seek 0ml @04:40:58.77 Disinfecting external Intake with mfbkill @04:40:58.90 CSV.dial CSR @04:41:00.38 Pulling 8ml of CSR.mfbkill @04:41:00.39 CSR.select :mfbkill @04:41:00.51 CS.seek 8ml @04:41:20.40 delay 5 seconds @04:41:25.43 CSR.select :air @04:41:25.55 CS.seek 10ml @04:41:31.31 CSV.dial CBV @04:41:31.33
CBV.dial CSV @04:41:32.46 CTV.dial :intake @04:41:33.29 Intake.select :open @04:41:33.41 CS.seek 2ml @04:41:54.43 Intake.select :close @04:41:54.54 CTV.dial :air @04:41:55.37 CS.reconfigure CSconfig @04:41:55.42 CS.seek 0ml @04:42:01.46 Flushing lower manifold's waste1 port @04:42:01.47 CSV.dial CSR @04:42:02.64 Pulling 4ml of CSR.mfbkill @04:42:02.65 CSR.select :mfbkill @04:42:02.77 CS.seek 4ml @04:42:14.18 delay 5 seconds @04:42:19.21 CSR.select :air @04:42:19.34 CS.reconfigure CSfast @04:42:19.38 CS.seek 10ml @04:42:34.55 CSV.dial CTV @04:42:34.58
CTV.dial CSV @04:42:36.12 CBV.dial :waste1 @04:42:36.64 CS.seek 0ml @04:43:01.27 Flushing lower manifold's waste2b port @04:43:01.28 CSV.dial CSR @04:43:01.83 Pulling 4ml of CSR.mfbkill @04:43:01.84 CSR.select :mfbkill @04:43:01.97 CS.reconfigure CSconfig @04:43:02.02 CS.seek 4ml @04:43:13.21 delay 5 seconds @04:43:18.24 CSR.select :air @04:43:18.37 CS.reconfigure CSfast @04:43:18.41 CS.seek 10ml @04:43:34.34 CSV.dial CTV @04:43:34.36
CTV.dial CSV @04:43:34.88 CBV.dial :waste2b @04:43:35.74 CS.seek 0ml @04:44:00.18 Killing upper manifold @04:44:00.19 CSV.dial CSR @04:44:00.74 Pulling 4ml of CSR.mfbkill @04:44:00.75 CSR.select :mfbkill @04:44:00.88 CS.reconfigure CSconfig @04:44:00.93 CS.seek 4ml @04:44:12.09 delay 5 seconds @04:44:17.12 CSR.select :air @04:44:17.25 CS.reconfigure CSfast @04:44:17.29 CS.seek 10ml @04:44:32.49 CSV.dial CBV @04:44:32.52
CBV.dial CSV @04:44:33.70 CTV.dial :air @04:44:35.18 CS.seek 0ml @04:44:59.65 delay 7 seconds @04:45:06.69 CS.seek 3ml @04:45:14.78 CSV.dial :waste @04:45:15.27 CS.seek 0ml @04:45:23.28 Flushing upper manifold @04:45:23.42 CSV.dial :flush @04:45:23.93 CS.seek 5ml @04:45:36.65 delay 5 seconds @04:45:41.68 CSV.dial :air @04:45:42.24 CS.seek 7ml @04:45:47.96 CSV.dial CBV @04:45:47.99
CBV.dial CSV @04:45:49.12 CTV.dial :air @04:45:49.14 CS.seek 0ml @04:46:06.81 delay 7 seconds @04:46:13.85 CS.seek 3ml @04:46:22.00 CSV.dial :waste @04:46:22.51 CS.seek 0ml @04:46:30.74 CSV.dial :flush @04:46:31.23 CS.seek 3ml @04:46:39.53 delay 5 seconds @04:46:44.56 CSV.dial :air @04:46:45.10 CS.seek 5ml @04:46:50.90 CSV.dial :air @04:46:50.92 CS.seek 0ml @04:47:03.84 delay 7 seconds @04:47:10.88 CS.seek 3ml @04:47:19.03 CSV.dial :waste @04:47:19.83 CS.seek 0ml @04:47:27.94 Flushing lower manifold's waste1 port @04:47:28.07 CSV.dial :flush @04:47:28.59 CS.seek 5ml @04:47:41.31 delay 5 seconds @04:47:46.34 CSV.dial :air @04:47:47.02 CS.seek 7ml @04:47:52.81 CSV.dial CTV @04:47:52.85
CTV.dial CSV @04:47:54.34 CBV.dial :waste1 @04:47:54.84 CS.seek 0ml @04:48:12.31 Flushing lower manifold's waste2b port @04:48:12.44 CSV.dial :flush @04:48:13.26 CS.seek 5ml @04:48:26.00 delay 5 seconds @04:48:31.03 CSV.dial :air @04:48:31.60 CS.seek 7ml @04:48:37.34 CSV.dial CTV @04:48:37.38
CTV.dial CSV @04:48:37.86 CBV.dial :waste2b @04:48:38.71 CS.seek 0ml @04:48:56.34 Disinfecting external Intake with kill @04:48:56.47 CSV.dial CSR @04:48:57.69 Pulling 8ml of CSR.kill @04:48:57.70 CSR.select :kill @04:48:57.83 CS.seek 8ml @04:49:17.69 delay 5 seconds @04:49:22.72 CSR.select :air @04:49:22.85 CS.seek 10ml @04:49:28.69 CSV.dial CBV @04:49:28.73
CBV.dial CSV @04:49:29.89 CTV.dial :intake @04:49:30.70 Intake.select :open @04:49:30.82 CS.seek 2ml @04:49:51.91 Intake.select :close @04:49:52.02 CTV.dial :air @04:49:52.84 CS.reconfigure CSconfig @04:49:52.89 CS.seek 0ml @04:49:58.97 CSV.dial CSR @04:50:00.14 Pulling 5ml of CSR.flush @04:50:00.15 CSR.select :flush @04:50:00.27 CS.seek 5ml @04:50:14.00 delay 5 seconds @04:50:19.03 CSR.select :air @04:50:19.16 CS.seek 10ml @04:50:32.91 CSV.dial CBV @04:50:34.15 CS.seek 0ml @04:51:00.72 Garaging FlushPuck in Collection Clamp @04:51:00.80 Elbow.reconfigure TightElbow @04:51:00.85 Elbow.seek CC @04:51:01.09 Elbow.reconfigure LooseElbow @04:51:01.20 Forearm.seek CC @04:51:02.17 Hand.close @04:51:02.80 CC.seek :open @04:51:07.16 Forearm.seek :retract @04:51:08.17 Elbow.reconfigure TightElbow @04:51:08.22 Elbow.seek :garage @04:51:11.45 Elbow.reconfigure LooseElbow @04:51:11.56 Forearm.reconfigure TightForearm @04:51:11.60 Forearm.seek :garage @04:51:12.75 Hand.open @04:51:13.49 Forearm.reconfigure LooseForearm @04:51:13.54 Forearm.seek :retract @04:51:14.55 Starting initialPurge @04:51:14.56 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @04:51:14.64 Hand.open @04:51:14.99 Elbow.reconfigure TightElbow @04:51:15.03 Elbow.seek FlushPuck::Garage @04:51:15.23 Elbow.reconfigure LooseElbow @04:51:15.34 Forearm.seek :garage @04:51:16.31 Hand.close @04:51:17.05 Forearm.seek :retract @04:51:18.06 Elbow.reconfigure TightElbow @04:51:18.11 Elbow.seek CC @04:51:20.98 Elbow.reconfigure LooseElbow @04:51:21.08 Forearm.reconfigure TightForearm @04:51:21.13 Forearm.seek CC @04:51:22.45 CC.seek :closed @04:51:26.72 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.07 @04:51:26.79 Hand.open @04:51:27.49 Forearm.reconfigure LooseForearm @04:51:27.53 Forearm.seek :retract @04:51:29.27 Flushing the Collection Valve::Manifold @04:51:29.40 CSV.dial CSR @04:51:30.56 Pulling 5ml of CSR.flush @04:51:30.57 CSR.select :flush @04:51:30.69 CS.seek 5ml @04:51:44.44 delay 5 seconds @04:51:49.47 CSR.select :air @04:51:49.63 CS.reconfigure CSfast @04:51:49.68 CS.seek 7.5ml @04:51:56.57 CSV.dial :waste @04:51:57.99 CS.reconfigure CSconfig @04:51:58.04 CS.seek 0ml @04:52:18.16 Purging external Intake @04:52:18.29 CSV.dial :flush @04:52:18.78 CS.reconfigure CSfast @04:52:18.83 CS.seek 10ml @04:52:43.22 delay 5 seconds @04:52:48.25 CSV.dial CBV @04:52:48.28
CBV.dial CSV @04:52:49.11 CTV.dial :intake @04:52:49.94 Intake.select :open @04:52:50.06 CS.seek 0ml @04:53:16.22 Intake.select :close @04:53:16.33 CTV.dial :air @04:53:17.16 Flushing upper manifold @04:53:17.29 CSV.dial :flush @04:53:18.11 CS.seek 5ml @04:53:30.94 delay 5 seconds @04:53:35.97 CSV.dial :air @04:53:36.53 CS.seek 7ml @04:53:42.19 CSV.dial CBV @04:53:42.21
CBV.dial CSV @04:53:43.35 CTV.dial :air @04:53:43.37 CS.seek 0ml @04:54:00.76 delay 7 seconds @04:54:07.80 CS.seek 3ml @04:54:15.82 CSV.dial :waste @04:54:16.31 CS.seek 0ml @04:54:24.72 CSV.dial :flush @04:54:25.20 CS.seek 3ml @04:54:33.26 delay 5 seconds @04:54:38.29 CSV.dial :air @04:54:38.84 CS.seek 5ml @04:54:44.85 CSV.dial :air @04:54:44.87 CS.seek 0ml @04:54:57.48 delay 7 seconds @04:55:04.52 CS.seek 3ml @04:55:12.54 CSV.dial :waste @04:55:13.34 CS.seek 0ml @04:55:21.38 Garaging FlushPuck in Collection Clamp @04:55:21.46 Elbow.reconfigure TightElbow @04:55:21.51 Elbow.seek CC @04:55:21.74 Elbow.reconfigure LooseElbow @04:55:21.84 Forearm.seek CC @04:55:22.84 Hand.close @04:55:23.43 CC.seek :open @04:55:27.79 Forearm.seek :retract @04:55:28.81 Elbow.reconfigure TightElbow @04:55:28.86 Elbow.seek :garage @04:55:31.99 Elbow.reconfigure LooseElbow @04:55:32.09 Forearm.reconfigure TightForearm @04:55:32.14 Forearm.seek :garage @04:55:33.32 Hand.open @04:55:34.00 Forearm.reconfigure LooseForearm @04:55:34.04 Forearm.seek :retract @04:55:35.07 Completed [wcrphase] begun 04:00:26 @04:55:35.08 Preparing to sleep until 10:00 28-may-20 @04:55:35.15 SS.coast @04:55:35.17
Asleep til 10:00 28-may-20 @04:55:35.25 Continuing at 10:00 @04:55:35.28 CC.coast @04:55:35.38 CS.coast @04:55:35.45 PC.coast @04:55:35.46 GoodNews.email "Then, in tube 4: coreNEG\n/home/esp/esp2/mission/20invasivedeployfinal.rb:171\n{1 => 22, 2 => 22, 3 => 3, 4 => 19}\nCan@04:55:35, 9.4C, 51% humidity, 14.4psia, 12.979V, 0.500A, 0.500A avg, 6.48W",Subject: "Asleep until 10:00" @04:55:35.55 Forearm.coast @04:55:36.27 Elbow.coast @04:55:36.70 Gate.power :core,:OFF @04:55:36.72 Gate.power :raw,:OFF @04:55:36.73 Shutdown @10:00:00.05
Powering core bus @10:00:04.75 Gate.power :raw,:ON @10:00:05.42 SPE.coast @10:00:05.49 SPE.configure SPEconfig @10:00:05.58 AS.configure ASconfig @10:00:05.69 SS.configure SSconfig @10:00:05.79 CC.configure CCconfig @10:00:05.88 CS.configure CSconfig @10:00:05.98 PC.configure PCconfig @10:00:06.08 PS.configure PSconfig @10:00:06.20 Forearm.configure LooseForearm @10:00:06.30 Elbow.configure LooseElbow @10:00:06.42 SC.configure SCloose @10:00:06.52 SE.configure SEconfig @10:00:06.57 CH.coast @10:00:06.62 CH.configure CHconfig @10:00:06.66 PH.coast @10:00:06.72 PH.configure PHconfig @10:00:06.76 PCR.off @10:00:06.79 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:06.82 PCR.caltemp= 2.8814,0.0372,0 @10:00:06.85 All dwarf controllers running firmware version 3.75 @10:00:06.96 Valve.reset @10:00:07.13 PCR.off @10:00:07.17 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:07.20 PCR.caltemp= 2.8814,0.0372,0 @10:00:07.43 Intake.select :close @10:00:07.45 PSV.dial :air @10:00:07.46 Exhaust.select :close @10:00:07.49 CTV.dial :air @10:00:07.68 PS.reconfigure PSconfig @10:00:07.76 PS.home.seek 0ml @10:00:07.80 CSV.dial :waste1 @10:00:07.86
Forearm.reconfigure LooseForearm @10:00:07.98 Forearm.home.seek :retract @10:00:08.00 SSV.dial :spit,avoiding: :exhaust @10:00:08.78 Hand.close @10:00:08.83
Elbow.jog 1500 @10:00:08.94 CS.reconfigure CSfast @10:00:08.97 SS.reconfigure SSconfig @10:00:09.06 CS.home.seek 0ml @10:00:09.10 SS.home.seek 0ml @10:00:09.54
Elbow.reconfigure LooseElbow @10:00:09.58 Elbow.home.seek :clear @10:00:13.02 SE.reconfigure SEconfig @10:00:13.09 SE.home.seek :down @10:00:13.84 SC.reconfigure SCsloppy @10:00:13.89 SC.home.seek :initial @10:00:16.94 PS.coast @10:00:19.48
Forearm.seek :retract @10:00:19.56 Elbow.seek :hammer @10:00:20.84 Forearm.reconfigure TightForearm @10:00:20.89 Forearm.seek 5 + 350 counts @10:00:22.71 Forearm.seek :retract @10:00:23.93 PC.reconfigure PCconfig @10:00:23.95
CC.reconfigure CCconfig @10:00:24.05 PC.home.seek :open @10:00:24.07
CC.home.seek :open @10:00:27.61 Phase [coreNEG] @10:00:27.63 GoodNews.email "At 10:00:27 began in tube 4: coreNEG\n/home/esp/esp2/mission/20invasivedeployfinal.rb:171\n{1 => 22, 2 => 22, 3 => 3, 4 => 19}\nCan@10:00:27, 8.6C, 52% humidity, 14.3psia, 12.994V, 0.664A, 0.657A avg, 8.62W",Subject: "Started 20invasivedeployfinal phase coreNEG" @10:00:27.65CEST28-May-20
===> STARTED coreNEG (phase #21) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @10:00:27.69 Starting initialPurge @10:00:27.70 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:00:27.78 Hand.open @10:00:28.58 Elbow.reconfigure TightElbow @10:00:28.63 Elbow.seek FlushPuck::Garage @10:00:30.61 Elbow.reconfigure LooseElbow @10:00:30.71 Forearm.reconfigure LooseForearm @10:00:30.76 Forearm.seek :garage @10:00:31.75 Hand.close @10:00:32.58 Forearm.seek :retract @10:00:33.61 Elbow.reconfigure TightElbow @10:00:33.66 Elbow.seek CC @10:00:36.63 Elbow.reconfigure LooseElbow @10:00:36.84 Forearm.reconfigure TightForearm @10:00:36.96 Forearm.seek CC @10:00:38.70 CC.seek :closed @10:00:43.25 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.27 @10:00:43.44 Hand.open @10:00:44.40 Forearm.reconfigure LooseForearm @10:00:44.49 Forearm.seek :retract @10:00:45.58 Flushing the Collection Valve::Manifold @10:00:45.86 CSV.dial CSR @10:00:47.47 Pulling 5ml of CSR.flush @10:00:47.49 CSR.select :flush @10:00:47.67 CS.reconfigure CSconfig @10:00:47.76 CS.seek 5ml @10:01:01.59 delay 5 seconds @10:01:06.62 CSR.select :air @10:01:06.90 CS.reconfigure CSfast @10:01:07.00 CS.seek 7.5ml @10:01:14.02 CSV.dial :waste @10:01:15.52 CS.reconfigure CSconfig @10:01:15.62 CS.seek 0ml @10:01:35.92 Purging external Intake @10:01:36.20 CSV.dial :flush @10:01:36.73 CS.reconfigure CSfast @10:01:36.78 CS.seek 10ml @10:02:01.59 delay 5 seconds @10:02:06.62 CSV.dial CBV @10:02:06.65
CBV.dial CSV @10:02:07.51 CTV.dial :intake @10:02:08.32 Intake.select :open @10:02:08.44 CS.seek 0ml @10:02:34.87 Intake.select :close @10:02:34.98 CTV.dial :air @10:02:35.81 Flushing upper manifold @10:02:35.94 CSV.dial :flush @10:02:36.76 CS.seek 5ml @10:02:49.65 delay 5 seconds @10:02:54.68 CSV.dial :air @10:02:55.91 CS.seek 7ml @10:03:01.65 CSV.dial CBV @10:03:01.67
CBV.dial CSV @10:03:02.81 CTV.dial :air @10:03:02.83 CS.seek 0ml @10:03:20.37 delay 7 seconds @10:03:27.41 CS.seek 3ml @10:03:35.50 CSV.dial :waste @10:03:36.01 CS.seek 0ml @10:03:44.27 CSV.dial :flush @10:03:44.76 CS.seek 3ml @10:03:52.84 delay 5 seconds @10:03:57.87 CSV.dial :air @10:03:58.55 CS.seek 5ml @10:04:04.37 CSV.dial :air @10:04:04.39 CS.seek 0ml @10:04:17.34 delay 7 seconds @10:04:24.38 CS.seek 3ml @10:04:32.53 CSV.dial :waste @10:04:33.32 CS.seek 0ml @10:04:41.50 Garaging FlushPuck in Collection Clamp @10:04:41.58 Elbow.reconfigure TightElbow @10:04:41.63 Elbow.seek CC @10:04:41.89 Elbow.reconfigure LooseElbow @10:04:42.00 Forearm.seek CC @10:04:43.02 Hand.close @10:04:43.58 CC.seek :open @10:04:47.94 Forearm.seek :retract @10:04:48.96 Elbow.reconfigure TightElbow @10:04:49.01 Elbow.seek :garage @10:04:52.16 Elbow.reconfigure LooseElbow @10:04:52.26 Forearm.reconfigure TightForearm @10:04:52.31 Forearm.seek :garage @10:04:53.47 Hand.open @10:04:54.15 Forearm.reconfigure LooseForearm @10:04:54.19 Forearm.seek :retract @10:04:55.35 Starting mfb @10:04:55.36 pcrBAC wcr sampling at most 1500ml @10:04:55.40 BACfans.email "Started mfb in tube 4 at 10:04:55CEST28-May-20\n{1 => 22, 2 => 22, 3 => 3, 4 => 19}\nCan@10:00:27, 8.6C, 52% humidity, 14.3psia, 12.994V, 0.664A, 0.657A avg, 8.62W",Subject: "pcrBAC wcr sampling at most 1500ml" @10:04:55.48
Loading sh1 puck from tube 4 into Collection Clamp @10:04:55.60 Readying the MFB @10:04:55.67 Homing MFB Syringe @10:04:55.68 AFV.select :holdingCoil @10:04:55.94 ATV.dial :air2 @10:04:55.96
SC.reconfigure SCtight @10:04:56.06 SC.seek 4 @10:04:57.46 AS.reconfigure ASconfig @10:04:57.53 AS.home.seek :home @10:04:59.70
SC.reconfigure SCloose @10:05:00.12 Elbow.reconfigure TightElbow @10:05:00.25 Elbow.seek 4 @10:05:02.59 Forearm.reconfigure TightForearm @10:05:02.69 Forearm.seek 4 + 700 counts @10:05:04.38 AFV.select :carrierSolution @10:05:04.51 delay 2 seconds @10:05:05.47
SE.reconfigure SEpick @10:05:05.59 SE.seek beamBreak @10:05:06.62 AS.seek 1ml @10:05:07.18
SE.configure SEconfig @10:05:07.66 Elbow.seek 4 @10:05:08.15 Forearm.seek 4 + 300 counts @10:05:08.53 Forearm.reconfigure LooseForearm @10:05:08.62 Hand.close @10:05:09.42 SE.seek :down @10:05:09.95
Forearm.seek :retract @10:05:10.69 SE.coast @10:05:10.97
Elbow.seek CC @10:05:12.80 Elbow.reconfigure LooseElbow @10:05:13.11 Forearm.reconfigure TightForearm @10:05:13.21 Forearm.seek CC @10:05:14.90 CC.seek :closed @10:05:19.30 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.21 @10:05:19.32 Hand.open @10:05:20.13 Forearm.reconfigure LooseForearm @10:05:20.24 Forearm.seek :retract @10:05:21.31 SC.coast @10:05:21.39 skipping prime and sample collection @10:05:21.42 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:05:21.44 Intake.select :close @10:05:21.62 CSV.dial :waste @10:05:21.82 CS.seek 0ml @10:05:22.00 CSV.dial :air @10:05:22.16
Calibrating pressure and temperature sensors @10:05:22.27 CTV.dial :air @10:05:22.31 SSV.dial :spit,avoiding: :exhaust @10:05:22.35 SS.reconfigure SSfast @10:05:22.46 SS.seek 5ml @10:05:23.09 CS.seek 10ml @10:05:25.22 SSV.dial CBV @10:05:25.28
CBV.dial SSV @10:05:26.83 delay 3.8 seconds @10:05:30.91 SSV.dial :spit @10:05:32.50 SS.seek 0ml @10:05:35.25 SSV.dial CBV @10:05:36.76 SSV.dial CBV,avoiding: :exhaust @10:05:36.81
CBV.dial SSV @10:05:36.85 CTV.dial :air @10:05:36.88 delay 15 seconds @10:05:51.91 SSV.dial :spit,avoiding: :exhaust @10:05:53.45 CTV.dial CSV,avoiding: :intake @10:05:53.53
SS.reconfigure SSexhale @10:05:53.75 SS.seek 0ml @10:05:53.87 SSV.dial CBV,avoiding: :exhaust @10:05:55.50 CBV.dial between SSV and waste2 @10:05:57.48 CTV.dial CSV,avoiding: :air @10:05:57.55
CSV.dial CTV @10:05:58.10 CS.reconfigure CSpressurize @10:05:58.19 CS.seek 0ml @10:05:59.60 delay 1 second @10:06:00.63 AFV.select :holdingCoil @10:06:00.76 delay 1 second @10:06:01.81 ATV.dial :waste @10:06:02.89 AS.reconfigure ASnorm @10:06:02.99 AS.home.seek :empty @10:06:24.44
CSV.dial :air @10:06:24.95 CS.reconfigure CSfast @10:06:24.99 CS.seek 10ml @10:06:49.72 CSV.dial between air and CTV @10:06:50.07 CS.seek 3.344ml @10:06:55.58 ATV.dial :waste @10:06:55.59 ARV.dial between carrier and air1 @10:06:55.69 ASV.dial :waste1 @10:07:07.00 CTV.dial CSV,avoiding: :air @10:07:07.03
CSV.dial CTV @10:07:07.37 CS.reconfigure CSpressurize @10:07:07.41 CS.seek 0ml @10:07:16.60 CSV.dial :air @10:07:17.11 CS.reconfigure CSfast @10:07:17.15 CS.seek 10ml @10:07:41.59 CSV.dial between air and CTV @10:07:41.96 CS.seek 2.08ml @10:08:01.73 CTV.dial CSV,avoiding: :air @10:08:01.75
CSV.dial CTV @10:08:02.09 CS.reconfigure CSpressurize @10:08:02.13 CS.seek 0ml @10:08:03.40 BubblePoint: Intake @69.5psia, Outlet @14.3psia @10:08:03.41 CBV.dial SSV @10:08:03.75 SS.reconfigure SSconfig @10:08:03.80 SS.seek 10ml @10:08:09.81 delay 15 seconds @10:08:24.94 Evacuated: Intake @28.7psia, Outlet @28.8psia @10:08:24.95 SSV.dial :spit,avoiding: :exhaust @10:08:26.41 SS.reconfigure SSexhale @10:08:26.46 SS.seek 0ml @10:08:31.28 SS.reconfigure SSfast @10:08:31.33 SS.seek 5ml @10:08:33.98 SSV.dial CBV,avoiding: :exhaust @10:08:34.00
CBV.dial SSV @10:08:35.44 CTV.dial :air,avoiding: :intake @10:08:36.89 CSV.dial CTV @10:08:36.94
SS.seek 10ml @10:08:37.94 CSV.dial :air @10:08:38.55 CS.reconfigure CSfast @10:08:38.60 CS.seek 0ml @10:08:39.85
SSV.dial :spit,avoiding: :exhaust @10:08:41.30 SS.reconfigure SSexhale @10:08:41.35 SS.seek 0ml @10:08:44.00 CS.coast @10:08:46.26
SS.reconfigure SSfast @10:08:46.31 SS.seek 5ml @10:08:49.17 SSV.dial CBV,avoiding: :exhaust @10:08:49.20
CBV.dial SSV @10:08:51.65 SSV.dial :spit,avoiding: :exhaust @10:08:53.08 SS.reconfigure SSconfig @10:08:53.13 SS.seek 0ml @10:08:56.12 SS.coast @10:08:56.17 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:08:56.18 Intake.select :close @10:08:56.93 CSV.dial :waste @10:08:57.06 SSV.dial CBV,avoiding: :exhaust @10:08:57.09
CBV.dial SSV @10:08:57.83 CS.seek 0ml @10:08:57.90 CSV.dial :air @10:08:58.55
CTV.dial :air @10:08:58.57 delay 15 seconds @10:08:58.72 CS.seek 10ml @10:09:13.59
SSV.dial :spit,avoiding: :exhaust @10:09:15.03 CTV.dial CSV,avoiding: :intake @10:09:15.07
SS.reconfigure SSexhale @10:09:15.18 SS.seek 0ml @10:09:15.25 SSV.dial CBV,avoiding: :exhaust @10:09:23.42 CBV.dial between SSV and waste2 @10:09:23.87 CTV.dial CSV,avoiding: :air @10:09:23.89
CSV.dial CTV @10:09:24.39 CS.reconfigure CSpressurize @10:09:24.43 CS.seek 0ml @10:09:50.63 CSV.dial :air @10:09:51.15 CS.reconfigure CSfast @10:09:51.19 CS.seek 10ml @10:10:15.57 CSV.dial between air and CTV @10:10:15.92 CS.seek 3.324ml @10:10:32.58 CTV.dial CSV,avoiding: :air @10:10:32.60
CSV.dial CTV @10:10:32.94 CS.reconfigure CSpressurize @10:10:32.98 CS.seek 0ml @10:10:42.10 CSV.dial :air @10:10:42.60 CS.reconfigure CSfast @10:10:42.66 CS.seek 10ml @10:11:06.94 CSV.dial between air and CTV @10:11:07.30 CS.seek 2.073ml @10:11:27.04 CTV.dial CSV,avoiding: :air @10:11:27.07
CSV.dial CTV @10:11:27.41 CS.reconfigure CSpressurize @10:11:27.45 CS.seek 0ml @10:11:28.85 BubblePoint: Intake @70.2psia, Outlet @14.8psia @10:11:28.86 CBV.dial SSV @10:11:29.23 SS.reconfigure SSconfig @10:11:29.28 SS.seek 10ml @10:11:35.28 delay 15 seconds @10:11:50.41 Evacuated: Intake @28.8psia, Outlet @28.9psia @10:11:50.42 SSV.dial :spit,avoiding: :exhaust @10:11:51.86 SS.reconfigure SSexhale @10:11:51.92 SS.seek 0ml @10:11:56.92 SS.reconfigure SSfast @10:11:56.97 SS.seek 5ml @10:11:59.83 SSV.dial CBV,avoiding: :exhaust @10:11:59.86
CBV.dial SSV @10:12:01.30 CTV.dial :air,avoiding: :intake @10:12:02.77 CSV.dial CTV @10:12:02.81
SS.seek 10ml @10:12:03.81 CSV.dial :air @10:12:04.32 CS.reconfigure CSfast @10:12:04.37 CS.seek 0ml @10:12:05.64
SSV.dial :spit,avoiding: :exhaust @10:12:07.75 SS.reconfigure SSexhale @10:12:07.81 SS.seek 0ml @10:12:09.51 CS.coast @10:12:12.75
SS.reconfigure SSfast @10:12:12.81 SS.seek 5ml @10:12:15.67 SSV.dial CBV,avoiding: :exhaust @10:12:15.69
CBV.dial SSV @10:12:18.15 SSV.dial :spit,avoiding: :exhaust @10:12:19.60 SS.reconfigure SSconfig @10:12:19.65 SS.seek 0ml @10:12:22.88 SS.coast @10:12:22.92 Lysing: Adding 1.5ml lysis @10:12:22.93 CBV.dial between CSV and waste1,avoiding: :DBV @10:12:24.57 CTV.dial CSV @10:12:26.02 CSV.dial CSR @10:12:26.88 Pulling 1ml of CSR.lysis @10:12:26.89 CSR.select :lysis @10:12:27.01 CS.reconfigure CSconfig @10:12:27.06 CS.seek 1ml @10:12:30.66 delay 5 seconds @10:12:35.69 CSR.select :air @10:12:35.87 CS.seek 6ml @10:12:49.57 delay 2 seconds @10:12:51.59 CSV.dial :waste1 @10:12:52.99 CS.reconfigure CSfast @10:12:53.05 CS.seek :empty @10:13:08.10 CSV.dial CSR @10:13:09.55 Pulling 1.5ml of CSR.lysis @10:13:09.56 CSR.select :lysis @10:13:09.68 CS.reconfigure CSconfig @10:13:09.72 CS.seek 1.5ml @10:13:14.57 delay 5 seconds @10:13:19.60 CSR.select :air @10:13:19.77 CS.seek 4.5ml @10:13:28.42 delay 2 seconds @10:13:30.44 CSV.dial CTV @10:13:30.94 CS.seek 1.275ml @10:13:40.21 Heating sh1 puck from tube 4 in Collection Clamp to 85C for 5:00 @10:13:40.23 CH.seek 85C,8:00 @10:16:59.19 CH.hold 5:00,85C @10:21:59.36 Collection Puck Heater at 85.1C after lysing @10:21:59.38 CS.seek 0ml @10:22:03.88 CTV.dial between spare and air,avoiding: :intake @10:22:05.03 CSV.dial :air @10:22:05.53 CS.seek 5ml @10:22:19.27 CSV.dial CTV @10:22:19.77 delay 2 seconds @10:22:21.79 CSV.dial :air @10:22:22.30 delay 2 seconds @10:22:24.32 CSV.dial CTV @10:22:24.82 delay 2 seconds @10:22:26.84 CSV.dial :air @10:22:27.35 delay 2 seconds @10:22:29.38 CS.seek 0ml @10:22:43.18 Recovering Lysate into Collection syringe @10:22:43.19 CSV.dial CBV @10:22:44.41 CBV.dial CSV @10:22:44.82 CS.seek 5ml @10:22:58.55 delay 5 seconds @10:23:03.57 CTV.dial :air @10:23:04.14 CS.seek 6.999ml @10:23:10.21 delay 5 seconds @10:23:15.23 CSV.dial :air2 @10:23:15.75 CS.seek 10ml @10:23:24.43 CSV.dial between air and CTV,avoiding: CBV @10:23:25.69 Lysing Complete @10:23:25.77 Storing sh1 puck in Collection Clamp to tube 3 @10:23:25.96 SC.reconfigure SCtight @10:23:26.01 SC.seek 3 @10:23:28.30 SC.reconfigure SCloose @10:23:28.52 Elbow.reconfigure TightElbow @10:23:28.57 Elbow.seek 3 @10:23:30.62 Forearm.reconfigure TightForearm @10:23:30.68 Forearm.seek 3 + 700 counts @10:23:31.75 SE.reconfigure SEpick @10:23:31.82 SE.seek beamBreak @10:23:34.73 SE.configure SEconfig @10:23:34.90 Elbow.reconfigure LimpElbow @10:23:34.95 Forearm.reconfigure LooseForearm @10:23:35.00 Forearm.seek 3 @10:23:35.30 Elbow.hold @10:23:35.37 Elbow.reconfigure TightElbow @10:23:35.48 SE.seek 2.06 Pucks below Top Plate @10:23:35.94 Forearm.force -20 @10:23:35.98 Forearm.seek 3 + 339 counts @10:23:36.31 Forearm.reconfigure TightForearm @10:23:37.05 Forearm.seek 3 + 700 counts @10:23:37.39 SE.seek 3.06 Pucks below Top Plate @10:23:37.97 Forearm.reconfigure LooseForearm @10:23:38.02 Forearm.seek :retract @10:23:38.94 Elbow.seek CC @10:23:40.62 Elbow.reconfigure LooseElbow @10:23:40.72 Forearm.seek CC @10:23:41.77 Hand.close @10:23:42.36 CC.seek :open @10:23:46.87 Forearm.seek :retract @10:23:47.91 Elbow.reconfigure TightElbow @10:23:47.96 Elbow.seek 3 @10:23:49.82 Elbow.reconfigure LimpElbow @10:23:49.87 Forearm.seek 3 @10:23:50.85 Elbow.hold @10:23:50.92 Elbow.reconfigure TightElbow @10:23:50.97 Hand.open @10:23:51.75 Forearm.force -20 @10:23:51.79 Forearm.seek 3 + 349 counts @10:23:52.13 Forearm.reconfigure TightForearm @10:23:52.17 Forearm.seek 3 + 700 counts @10:23:52.46 SE.seek :down @10:23:53.61
Forearm.reconfigure LooseForearm @10:23:53.66 Forearm.seek :retract @10:23:54.92 SE.coast @10:23:54.96
SC.coast @10:23:55.00 Tranferring lysate to PRV @10:23:55.01 CSV.dial :air @10:23:55.34 CS.seek 10ml @10:23:55.42 CSV.dial between air and CTV @10:23:55.77 AIV.dial :bypass @10:23:55.87 Beginning samplePassC @10:23:55.89 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:23:55.96 Hand.open @10:23:56.37 Elbow.seek FlushPuck::Garage @10:23:58.24 Elbow.reconfigure LooseElbow @10:23:58.35 Forearm.seek :garage @10:23:59.36 Hand.close @10:24:00.12 Forearm.seek :retract @10:24:01.13 Elbow.reconfigure TightElbow @10:24:01.18 Elbow.seek CC @10:24:04.10 Elbow.reconfigure LooseElbow @10:24:04.20 Forearm.reconfigure TightForearm @10:24:04.25 Forearm.seek CC @10:24:06.26 CC.seek :closed @10:24:10.52 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.16 @10:24:10.60 Hand.open @10:24:11.31 Forearm.reconfigure LooseForearm @10:24:11.36 Forearm.seek :retract @10:24:12.35 AWV.select :air @10:24:12.36 AIV.dial :bypass @10:24:12.37 PSV.dial PSR @10:24:13.85 Pulling 1.5ml of PSR.flush @10:24:13.86 PSR.select :flush @10:24:13.99 PS.seek 1.5ml @10:24:29.98 delay 5 seconds @10:24:35.01 PSV.dial :waste1 @10:24:35.52 PS.seek 0ml @10:24:51.51 PSV.dial PSR @10:24:52.01 PSR.select :air @10:24:52.13 PS.seek 2.5ml @10:25:18.24 PRV.dial CBV @10:25:18.26
CBV.dial PRV @10:25:19.16 PSV.dial :mixloop @10:25:20.26 CTV.dial :air,avoiding: :intake @10:25:20.27 PTV.dial :PRVmixing @10:25:20.35 PS.seek 0ml @10:25:46.54 PSV.dial :air @10:25:47.02 PS.seek 2.5ml @10:26:13.20 PSV.dial :mixloop @10:26:13.69 PS.seek 0ml @10:26:39.76 PSV.dial :air @10:26:40.24 PS.seek 2.5ml @10:27:06.38 PSV.dial :mixloop @10:27:06.86 PS.seek 0ml @10:27:33.01 Garaging FlushPuck in Collection Clamp @10:27:33.09 Elbow.reconfigure TightElbow @10:27:33.14 Elbow.seek CC @10:27:33.38 Elbow.reconfigure LooseElbow @10:27:33.49 Forearm.seek CC @10:27:34.53 Hand.close @10:27:35.12 CC.seek :open @10:27:39.50 Forearm.seek :retract @10:27:40.53 Elbow.reconfigure TightElbow @10:27:40.58 Elbow.seek :garage @10:27:43.79 Elbow.reconfigure LooseElbow @10:27:43.89 Forearm.reconfigure TightForearm @10:27:43.94 Forearm.seek :garage @10:27:45.12 Hand.open @10:27:45.82 Forearm.reconfigure LooseForearm @10:27:45.87 Forearm.seek :retract @10:27:46.92 Connecting PS to CS to transfer sample @10:27:46.93 CBV.dial AIV @10:27:46.94 AIV.dial :bypass @10:27:46.95 PSV.dial :mixloop @10:27:46.97 PTV.dial :PRVmixing @10:27:46.98 PRV.dial AIV @10:27:46.99 CTV.dial :air,avoiding: :intake @10:27:47.01 Loading lyfil puck from tube 4 into Collection Clamp @10:27:47.26 SC.reconfigure SCtight @10:27:47.31 SC.seek 4 @10:27:49.53 SC.reconfigure SCloose @10:27:50.46 Elbow.reconfigure TightElbow @10:27:50.51 Elbow.seek 4 @10:27:52.59 Forearm.reconfigure TightForearm @10:27:52.64 Forearm.seek 4 + 700 counts @10:27:53.74 SE.reconfigure SEpick @10:27:53.82 SE.seek beamBreak @10:27:55.38 SE.configure SEconfig @10:27:55.56 Elbow.seek 4 @10:27:55.95 Forearm.seek 4 + 300 counts @10:27:56.20 Forearm.reconfigure LooseForearm @10:27:56.25 Hand.close @10:27:56.89 SE.seek :down @10:27:57.12
Forearm.seek :retract @10:27:58.12 SE.coast @10:27:58.18
Elbow.seek CC @10:27:59.88 Elbow.reconfigure LooseElbow @10:27:59.98 Forearm.reconfigure TightForearm @10:28:00.03 Forearm.seek CC @10:28:01.39 CC.seek :closed @10:28:05.65 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.26 @10:28:05.66 Hand.open @10:28:06.33 Forearm.reconfigure LooseForearm @10:28:06.37 Forearm.seek :retract @10:28:07.40 SC.coast @10:28:07.44 CTV.dial CSV,avoiding: :intake @10:28:08.90 CSV.dial CTV @10:28:09.22 CS.seek 5ml @10:28:22.96 delay 1:20 @10:29:43.00 CS.seek 2.5ml @10:29:50.31 delay 40 seconds @10:30:30.35 CS.seek 0ml @10:30:37.75 delay 1:00 @10:31:37.78 PSV.dial :air @10:31:38.26 PS.seek 2.5ml @10:32:04.39 PSV.dial :mixloop @10:32:04.86 delay 3 seconds @10:32:07.88 PSV.dial :air @10:32:08.36 delay 2 seconds @10:32:10.38 PSV.dial :mixloop @10:32:10.86 delay 3 seconds @10:32:13.88 PSV.dial :waste1 @10:32:15.30 PS.seek :empty @10:32:41.51 CBV.dial :waste1 @10:32:42.02 delay 5 seconds @10:32:47.05 CBV.dial AIV @10:32:47.56 delay 10 seconds @10:32:57.59 CBV.dial :waste1 @10:32:58.10 delay 10 seconds @10:33:08.13 CBV.dial AIV @10:33:08.66 delay 10 seconds @10:33:18.69 CTV.dial CSV,avoiding: :intake @10:33:18.70 CBV.dial between AIV and waste1 @10:33:19.06 CSV.dial :air @10:33:19.56 CS.seek 10ml @10:33:46.12 CSV.dial CTV @10:33:46.64 CS.seek 0ml @10:34:13.16 CBV.dial :waste1 @10:34:13.49 delay 10 seconds @10:34:23.52 CTV.dial :air,avoiding: :intake @10:34:25.74 Storing lyfil puck in Collection Clamp to tube 3 @10:34:25.93 SC.reconfigure SCtight @10:34:25.98 SC.seek 3 @10:34:28.32 SC.reconfigure SCloose @10:34:28.55 Elbow.reconfigure TightElbow @10:34:28.60 Elbow.seek 3 @10:34:30.53 Forearm.reconfigure TightForearm @10:34:30.58 Forearm.seek 3 + 700 counts @10:34:31.66 SE.reconfigure SEpick @10:34:31.74 SE.seek beamBreak @10:34:34.58 SE.configure SEconfig @10:34:34.74 Elbow.reconfigure LimpElbow @10:34:34.79 Forearm.reconfigure LooseForearm @10:34:34.84 Forearm.seek 3 @10:34:35.12 Elbow.hold @10:34:35.19 Elbow.reconfigure TightElbow @10:34:35.30 SE.seek 3.07 Pucks below Top Plate @10:34:35.80 Forearm.force -20 @10:34:35.85 Forearm.seek 3 + 340 counts @10:34:36.18 Forearm.reconfigure TightForearm @10:34:36.23 Forearm.seek 3 + 700 counts @10:34:36.57 SE.seek 4.07 Pucks below Top Plate @10:34:37.30 Forearm.reconfigure LooseForearm @10:34:37.35 Forearm.seek :retract @10:34:38.24 Elbow.seek CC @10:34:39.94 Elbow.reconfigure LooseElbow @10:34:40.05 Forearm.seek CC @10:34:41.10 Hand.close @10:34:41.77 CC.seek :open @10:34:46.25 Forearm.seek :retract @10:34:47.32 Elbow.reconfigure TightElbow @10:34:47.37 Elbow.seek 3 @10:34:49.31 Elbow.reconfigure LimpElbow @10:34:49.36 Forearm.seek 3 @10:34:50.35 Elbow.hold @10:34:50.43 Elbow.reconfigure TightElbow @10:34:50.49 Hand.open @10:34:51.26 Forearm.force -20 @10:34:51.30 Forearm.seek 3 + 350 counts @10:34:51.62 Forearm.reconfigure TightForearm @10:34:51.67 Forearm.seek 3 + 700 counts @10:34:52.09 SE.seek :down @10:34:53.24
Forearm.reconfigure LooseForearm @10:34:53.29 Forearm.seek :retract @10:34:54.43 SE.coast @10:34:54.47
SC.coast @10:34:54.51 PSV.dial :air @10:34:55.79 PS.seek 0.499ml @10:35:01.74 delay 3 seconds @10:35:04.76 PSV.dial :mixloop @10:35:05.95 PRV.dial :air @10:35:06.44 delay 3 seconds @10:35:09.46 PRV.dial CBV @10:35:09.97 CBV.dial AIV @10:35:10.47 PRV.dial between CBV and air @10:35:10.83 PRV.dial :air @10:35:11.24 PS.reconfigure PSslow @10:35:11.29 PS.seek 0.599ml @10:35:13.80 PRV.dial :waste2 @10:35:14.61 PS.reconfigure PSconfig @10:35:14.65 PS.seek :empty @10:35:21.55 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:35:21.62 Hand.open @10:35:22.01 Elbow.seek FlushPuck::Garage @10:35:23.82 Elbow.reconfigure LooseElbow @10:35:23.93 Forearm.seek :garage @10:35:24.92 Hand.close @10:35:25.64 Forearm.seek :retract @10:35:26.66 Elbow.reconfigure TightElbow @10:35:26.71 Elbow.seek CC @10:35:29.62 Elbow.reconfigure LooseElbow @10:35:29.73 Forearm.reconfigure TightForearm @10:35:29.78 Forearm.seek CC @10:35:31.11 CC.seek :closed @10:35:35.38 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.10 @10:35:35.45 Hand.open @10:35:36.21 Forearm.reconfigure LooseForearm @10:35:36.26 Forearm.seek :retract @10:35:37.36 Clearing PML for modified lysate @10:35:37.37 CTV.dial :air,avoiding: :intake @10:35:37.38 PRV.dial :waste2 @10:35:37.39 PTV.dial :PRV,avoiding: :puck @10:35:38.21 delay 2 seconds @10:35:40.23 PTV.dial :PRVmixing,avoiding: :puck @10:35:41.03 delay 2 seconds @10:35:43.05 PSV.dial :PML @10:35:43.06 delay 2 seconds @10:35:45.09 PRV.dial :air @10:35:45.89 delay 2 seconds @10:35:47.97 PS.seek 1.499ml @10:36:03.99 delay 5 seconds @10:36:09.03 PS.seek 2.5ml @10:36:20.02 PSV.dial :air @10:36:20.51 PS.seek 0ml @10:36:46.64 mixing lysate with speDil @10:36:46.65 PSV.dial :PML @10:36:47.15 CBV.dial :PRV @10:36:47.16 PRV.dial :air @10:36:47.18 delay 2 seconds @10:36:49.20 PRV.dial :speDil @10:36:49.71 delay 2 seconds @10:36:51.79 PS.reconfigure PSslow1 @10:36:51.84 PS.seek 0.225ml @10:37:15.11 delay 5 seconds @10:37:20.13 PRV.dial CBV @10:37:20.96 delay 2 seconds @10:37:23.04 PS.seek 0.475ml @10:37:49.46 delay 5 seconds @10:37:54.49 PRV.dial :air @10:37:54.99 delay 2 seconds @10:37:57.02 PS.seek 0.965ml @10:38:46.86 delay 10 seconds @10:38:56.89 PRV.dial CBV @10:38:57.40 CTV.dial :air,avoiding: :intake @10:38:57.41 PSV.dial :air @10:38:57.91 PS.reconfigure PSconfig @10:38:57.97 PS.seek 2.5ml @10:39:14.40 delay 3 seconds @10:39:17.42 PSV.dial PTV @10:39:18.22 PTV.dial :PRV,avoiding: :puck @10:39:19.03 PS.seek 0ml @10:39:45.18 PSV.dial :air @10:39:46.00 PS.seek 2.5ml @10:40:12.12 delay 3 seconds @10:40:15.14 PSV.dial PTV @10:40:15.93 PTV.dial :PRV,avoiding: :puck @10:40:15.95 PS.seek 0ml @10:40:42.05 Pushing modified lysate into AIV Injection Loop @10:40:42.06 CBV.dial AIV @10:40:42.07 AIV.dial :bypass @10:40:42.08 PRV.dial CBV @10:40:42.10 PSV.dial :air @10:40:42.89 PS.seek 2.5ml @10:41:08.99 delay 3 seconds @10:41:12.01 PSV.dial :PML @10:41:12.49 PTV.dial :PRVmixing,avoiding: :puck @10:41:13.37 PS.reconfigure PSslow1 @10:41:13.42 PS.seek 1.74ml @10:42:30.81 delay 5 seconds @10:42:35.84 AIV.dial :sample @10:42:36.47 AWV.select :waste @10:42:36.64 PS.reconfigure PSslow2 @10:42:36.69 PS.seek 1.22ml @10:44:24.09 delay 15 seconds @10:44:39.12 AIV.dial :bypass @10:44:39.73 PS.reconfigure PSconfig @10:44:39.78 PS.seek 0ml @10:44:53.03 PSV.dial :air @10:44:53.52 PS.seek 2.5ml @10:45:19.62 PSV.dial :PML @10:45:20.10 PS.seek 0ml @10:45:46.21 AIV.dial :coilToMFB @10:45:46.22 AWV.select :air @10:45:46.39 Extracting Nucleic Acid from AIV @10:45:46.42
Beginning samplePassD @10:45:46.46 SPE.hold 2:46:40,55C @10:45:46.55 ASV.dial :SPEdelivery @10:45:46.61
AWV.select :air @10:45:46.63 AIV.dial :bypass @10:45:46.64 PSV.dial PSR @10:45:46.96 delay 20 seconds @10:45:47.85
Pulling 1.5ml of PSR.killMFB @10:45:47.86 PSR.select :killMFB @10:45:47.99 PS.seek 1.5ml @10:46:03.97 delay 5 seconds @10:46:06.99 primeLysateINT @10:46:07.00 AWV.select :air @10:46:07.01 AIV.dial :coilToMFB @10:46:07.03 ATV.dial AIV @10:46:08.88 AS.reconfigure ASslow1 @10:46:08.93 AS.seek 0.299ml @10:46:08.99
PSV.dial :waste1 @10:46:09.62 PS.seek 0ml @10:46:25.72 PSV.dial PSR @10:46:26.34 PSR.select :air @10:46:26.46 PS.seek 2.5ml @10:46:52.59 PRV.dial CBV @10:46:52.62
CBV.dial PRV @10:46:52.63 PSV.dial :mixloop @10:46:53.79 CTV.dial :air,avoiding: :intake @10:46:53.80 PTV.dial :PRVmixing @10:46:53.82 PS.seek 0ml @10:47:09.60 delay 10 seconds @10:47:19.63 ATV.dial :air2 @10:47:19.90
PSV.dial PSR @10:47:20.73 AS.seek 0.329ml @10:47:21.82
Pulling 1.5ml of PSR.flush PSR.select :flush @10:47:21.96 PS.seek 1.5ml @10:47:27.06 ATV.dial :waste2 @10:47:27.65 AS.reconfigure ASnorm @10:47:27.70 AS.seek :empty @10:47:37.97
delay 5 seconds @10:47:43.00 PSV.dial :waste1 @10:47:43.52 PS.seek 0ml @10:47:44.70 Lysate primed! @10:47:44.71 AFV.select :holdingCoil @10:47:44.72 ATV.dial :air2 @10:47:45.36 AS.reconfigure ASslow1 @10:47:45.41 AS.seek 0.069ml @10:47:59.62
PSV.dial PSR @10:47:59.82 delay 3 seconds @10:48:00.12
PSR.select :air @10:48:00.25 PS.seek 2.5ml @10:48:02.84 ATV.dial :AIV @10:48:03.72 AS.seek 0.469ml @10:48:26.50 PRV.dial CBV @10:48:26.53
CBV.dial PRV @10:48:26.55 PSV.dial :mixloop @10:48:27.70 CTV.dial :air,avoiding: :intake @10:48:27.71 PTV.dial :PRVmixing @10:48:27.73 PS.seek 0ml @10:48:53.81 PSV.dial PSR @10:48:55.01 Pulling 1.5ml of PSR.flush @10:48:55.02 PSR.select :flush @10:48:55.15 PS.seek 1.5ml @10:49:11.16 delay 5 seconds @10:49:16.19 PSV.dial :waste1 @10:49:16.71 PS.seek 0ml @10:49:24.40 delay 5 seconds @10:49:29.48 Solid Phase Extraction Heater at 44.5C @10:49:29.49 ATV.dial ASV @10:49:29.90 ASV.dial :SPEdelivery @10:49:29.98 AS.seek 0.041ml @10:49:32.75
PSV.dial PSR @10:49:33.26 PSR.select :air @10:49:33.39 PS.seek 2.5ml @10:49:59.50 PRV.dial CBV @10:49:59.53
CBV.dial PRV @10:49:59.54 PSV.dial :mixloop @10:50:00.67 CTV.dial :air,avoiding: :intake @10:50:00.68 PTV.dial :PRVmixing @10:50:00.70 PS.seek 0ml @10:50:26.91 PSV.dial PSR @10:50:28.11 Pulling 1.5ml of PSR.flush @10:50:28.12 PSR.select :flush @10:50:28.25 PS.seek 1.5ml @10:50:44.38 delay 5 seconds @10:50:49.41 PSV.dial :waste1 @10:50:49.92 PS.seek 0ml @10:50:56.34 delay 10 seconds @10:51:05.91
PSV.dial PSR @10:51:06.36 rinseLysateLineINT @10:51:06.37 AFV.select :carrierSolution @10:51:06.43
PSR.select :air @10:51:06.50 delay 2 seconds @10:51:06.56
PS.seek 2.5ml @10:51:08.53 AS.reconfigure ASconfig @10:51:08.59 AS.seek 1ml @10:51:32.66 PRV.dial CBV @10:51:32.69
CBV.dial PRV @10:51:32.70 PSV.dial :mixloop @10:51:33.83 CTV.dial :air,avoiding: :intake @10:51:33.84 PTV.dial :PRVmixing @10:51:33.86 PS.reconfigure PSslow @10:51:33.91 PS.seek 1.2ml @10:52:00.75 PSV.dial :waste1 @10:52:02.19 PS.reconfigure PSconfig @10:52:02.24 PS.seek 0ml @10:52:04.00 delay 1 second @10:52:05.02 AFV.select :holdingCoil @10:52:05.12 delay 1 second @10:52:06.14 ATV.dial AIV @10:52:06.70 AIV.dial :coilToMFB @10:52:06.71 AWV.select :waste @10:52:06.83 AS.reconfigure ASslow0 @10:52:07.57 AS.seek 0ml @10:52:15.25
PSV.dial :air @10:52:16.37 PS.seek 2.5ml @10:52:42.50 PSV.dial :mixloop @10:52:42.98 PS.reconfigure PSslow @10:52:43.02 PS.seek 0ml @10:53:34.19 PSV.dial :air @10:53:34.67 PS.reconfigure PSconfig @10:53:34.72 PS.seek 2.5ml @10:53:48.44 ATV.dial :air2 @10:53:49.11 AS.reconfigure ASslow1 @10:53:49.16 AS.seek 0.05ml @10:53:59.54 ATV.dial :AIV @10:54:00.37 AS.seek 0.01ml @10:54:00.85
PSV.dial :mixloop @10:54:01.35 PS.reconfigure PSslow @10:54:01.40 PS.seek 0ml @10:54:08.70 ATV.dial :waste @10:54:10.35 AS.seek :empty @10:54:12.76 ethanol wash @10:54:12.77 ATV.dial :air2 @10:54:14.05 AS.reconfigure ASslow2 @10:54:14.10 AS.seek 0.009ml @10:54:24.45 delay 3 seconds @10:54:27.48 Pulling 0.4ml of Ethanol Wash @10:54:27.49 ATV.dial :ethanol @10:54:28.32 AS.reconfigure ASslow1 @10:54:28.37 AS.seek 0.409ml @10:54:52.54
Cleaning Collection Syringe @10:54:52.55 CSV.dial CSR @10:54:53.09 Pulling 4ml of CSR.flush @10:54:53.10 CSR.select :flush @10:54:53.23 CS.seek 4ml @10:55:04.53 delay 5 seconds @10:55:09.56 CSR.select :air @10:55:09.69 CS.seek 10ml @10:55:26.03 CSV.dial CTV @10:55:26.07
CTV.dial CSV @10:55:27.61 CBV.dial :waste1 @10:55:28.13 CS.seek 0ml @10:55:49.12 delay 3 seconds @10:55:52.15 ATV.dial :air1 @10:55:52.55 delay 3 seconds @10:55:54.65
CSV.dial CSR @10:55:55.20 Pulling 4ml of CSR.flush @10:55:55.21 CSR.select :flush @10:55:55.34 CS.seek 4ml @10:55:55.63 AS.seek 0.434ml @10:56:00.95 delay 3 seconds @10:56:03.98 ATV.dial :ethanol @10:56:04.59 AS.seek 0.419ml @10:56:06.75
delay 5 seconds @10:56:07.86 delay 3 seconds @10:56:10.89 ATV.dial ASV @10:56:11.78
CSR.select :air @10:56:11.91 CS.seek 10ml @10:56:12.21 AS.seek 0.092ml @10:56:28.28 CSV.dial CTV @10:56:28.32
CTV.dial CSV @10:56:28.83 CBV.dial :waste1 @10:56:28.85 CS.seek 0ml @10:56:55.69 Garaging FlushPuck in Collection Clamp @10:56:55.76 Elbow.reconfigure TightElbow @10:56:55.81 Elbow.seek CC @10:56:56.06 Elbow.reconfigure LooseElbow @10:56:56.17 Forearm.seek CC @10:56:57.17 Hand.close @10:56:57.80 CC.seek :open @10:57:02.16 Forearm.seek :retract @10:57:03.17 Elbow.reconfigure TightElbow @10:57:03.22 Elbow.seek :garage @10:57:07.10 Elbow.reconfigure LooseElbow @10:57:07.20 Forearm.reconfigure TightForearm @10:57:07.25 Forearm.seek :garage @10:57:08.42 Hand.open @10:57:09.12 Forearm.reconfigure LooseForearm @10:57:09.17 Forearm.seek :retract @10:57:10.19 Whole Cell Archive sampling 1500ml @10:57:10.21 Loading wcr puck from tube 4 into Collection Clamp @10:57:10.48 SC.reconfigure SCtight @10:57:10.53 SC.seek 4 @10:57:12.91 SC.reconfigure SCloose @10:57:13.15 Elbow.reconfigure TightElbow @10:57:13.20 Elbow.seek 4 @10:57:15.28 Forearm.reconfigure TightForearm @10:57:15.33 Forearm.seek 4 + 700 counts @10:57:16.46 SE.reconfigure SEpick @10:57:16.54 SE.seek beamBreak @10:57:18.15 SE.configure SEconfig @10:57:18.23 delay 3 seconds @10:57:18.39 Elbow.seek 4 @10:57:18.72 Forearm.seek 4 + 300 counts @10:57:19.09 Forearm.reconfigure LooseForearm @10:57:19.14 Hand.close @10:57:19.82 SE.seek :down @10:57:20.05 Forearm.seek :retract @10:57:21.02 Elbow.seek CC @10:57:21.14 SE.coast @10:57:21.26 ATV.dial :waste2 @10:57:22.00 AS.reconfigure ASconfig @10:57:22.05 AS.seek :empty @10:57:22.73 Elbow.reconfigure LooseElbow @10:57:22.84 Forearm.reconfigure TightForearm @10:57:22.89 Forearm.seek CC @10:57:24.24 CC.seek :closed @10:57:27.72 ATV.dial :air2 @10:57:28.14 AS.reconfigure ASslow1 @10:57:28.19 AS.seek 1ml @10:57:28.50 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.38 @10:57:28.52 Hand.open @10:57:29.38 Forearm.reconfigure LooseForearm @10:57:29.43 Forearm.seek :retract @10:57:30.44 SC.coast @10:57:30.48 collectSample 1500ml w/bubble point of 55psi @10:57:30.50 Priming 7ml @10:57:30.51 Intake.select :close @10:57:30.52 Exhaust.select :close @10:57:30.53 SS.hold @10:57:31.49 Calibrating pressure sensors @10:57:31.50 CTV.dial :air @10:57:32.97 SSV.dial :spit,avoiding: :exhaust @10:57:32.99 SS.reconfigure SSfast @10:57:33.04 SS.seek 5ml @10:57:35.63 SSV.dial CBV @10:57:35.66 CBV.dial SSV @10:57:37.23 delay 3.8 seconds @10:57:41.18 SSV.dial :spit @10:57:42.64 SS.seek 0ml @10:57:45.32 SSV.dial CBV @10:57:46.77 CTV.dial :intake @10:57:47.60 SSV.dial CBV @10:57:47.62 CBV.dial SSV @10:57:47.65 Allowing 40s for 7.0ml of remaining 7.0ml @10:57:47.66 Intake.select :open @10:57:49.84 Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:57:49.86 SS.reconfigure SSinhale.with maxSpeed: 150 @10:57:49.90 SS.seek 7ml @10:57:55.52 Stroke completed -- waiting for pressure to equalize ... @10:58:09.51 Intake.select :close @10:58:09.67 SSV.dial :exhaust @10:58:10.50 Exhaust.select :open @10:58:10.62 SS.reconfigure SSexhale @10:58:10.67 SS.seek 0ml @10:58:14.24 Exhaust.select :close @10:58:14.35 Sampling 1500.0ml @10:58:14.37 Intake.select :close @10:58:14.38 Exhaust.select :close @10:58:14.39 SS.reconfigure SSconfig @10:58:14.43 SS.hold @10:58:14.66 CTV.dial :intake @10:58:14.67 SSV.dial CBV @10:58:14.71 CBV.dial SSV @10:58:15.57 Allowing 130s for 25.0ml of remaining 1500.0ml @10:58:15.58 Intake.select :open @10:58:17.76 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:58:17.78 SS.reconfigure SSinhale.with maxSpeed: 150 @10:58:17.83 SS.seek 25ml @10:58:21.69 Vacuum too high -- waiting up to 40s @10:58:24.05 Intake @32.1psia, vacuum from 20.0 to 24.0psi @10:58:24.07 SS.reconfigure SSinhale.with maxSpeed: 135 @10:58:24.12 SS.seek 25ml @10:58:26.76 Vacuum too high -- waiting up to 40s @10:58:28.92 Intake @32.0psia, vacuum from 20.0 to 24.0psi @10:58:28.94 SS.reconfigure SSinhale.with maxSpeed: 122 @10:58:28.98 SS.seek 25ml @10:58:31.60 Vacuum too high -- waiting up to 40s @10:58:34.01 SS.reconfigure SSinhale.with maxSpeed: 110 @10:58:34.06 SS.seek 25ml @10:58:36.79 Vacuum too high -- waiting up to 40s @10:58:39.17 SS.reconfigure SSinhale.with maxSpeed: 99 @10:58:39.22 SS.seek 25ml @10:58:42.01 Vacuum too high -- waiting up to 40s @10:58:44.38 Intake @31.9psia, vacuum from 20.0 to 24.0psi @10:58:44.40 SS.reconfigure SSinhale.with maxSpeed: 89 @10:58:44.45 SS.seek 25ml @10:58:46.85 Vacuum too high -- waiting up to 40s @10:58:49.19 Intake @31.9psia, vacuum from 20.0 to 24.0psi @10:58:49.21 SS.reconfigure SSinhale.with maxSpeed: 80 @10:58:49.26 SS.seek 25ml @10:58:53.08 Vacuum too high -- waiting up to 40s @10:58:55.23 SS.reconfigure SSinhale.with maxSpeed: 72 @10:58:55.28 SS.seek 25ml @10:58:57.54 Vacuum too high -- waiting up to 40s @10:58:59.95 SS.reconfigure SSinhale.with maxSpeed: 65 @10:59:00.00 SS.seek 25ml @10:59:02.66 Vacuum too high -- waiting up to 40s @10:59:05.02 Intake @31.9psia, vacuum from 20.0 to 24.0psi @10:59:05.04 SS.reconfigure SSinhale.with maxSpeed: 59 @10:59:05.09 SS.seek 25ml @10:59:07.79 Vacuum too high -- waiting up to 40s @10:59:09.90 Intake @31.8psia, vacuum from 20.0 to 24.0psi @10:59:09.92 SS.reconfigure SSinhale.with maxSpeed: 53 @10:59:09.97 SS.seek 25ml @10:59:12.71 Stroke completed -- waiting for pressure to equalize ... @10:59:18.43 Intake.select :close @10:59:18.59 SSV.dial :exhaust @10:59:19.44 Exhaust.select :open @10:59:19.56 SS.reconfigure SSexhale @10:59:19.61 SS.seek 0ml @10:59:29.31 Exhaust.select :close @10:59:29.46 SSV.dial CBV @10:59:29.48 CBV.dial SSV @10:59:30.35 Allowing 130s for 25.0ml of remaining 1475.0ml @10:59:30.36 Intake.select :open @10:59:32.55 Intake @31.4psia, vacuum from 20.0 to 24.0psi @10:59:32.57 SS.reconfigure SSinhale.with maxSpeed: 53 @10:59:32.61 SS.seek 25ml @10:59:37.70 Vacuum too high -- waiting up to 40s @10:59:40.11 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:59:40.13 SS.reconfigure SSinhale.with maxSpeed: 48 @10:59:40.17 SS.seek 25ml @10:59:44.25 Vacuum too high -- waiting up to 40s @10:59:46.13 Intake @31.6psia, vacuum from 20.0 to 24.0psi @10:59:46.15 SS.reconfigure SSinhale.with maxSpeed: 43 @10:59:46.20 SS.seek 25ml @10:59:50.73 Vacuum too high -- waiting up to 40s @10:59:52.84 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:59:52.86 SS.reconfigure SSinhale.with maxSpeed: 39 @10:59:52.90 SS.seek 25ml @10:59:57.26 Vacuum too high -- waiting up to 40s @10:59:59.13 Intake @31.7psia, vacuum from 20.0 to 24.0psi @10:59:59.15 SS.reconfigure SSinhale.with maxSpeed: 35 @10:59:59.20 SS.seek 25ml @11:00:06.01 Vacuum too high -- waiting up to 40s @11:00:08.11 SS.reconfigure SSinhale.with maxSpeed: 32 @11:00:08.16 SS.seek 25ml @11:00:16.19 Vacuum too high -- waiting up to 40s @11:00:18.30 Intake @31.6psia, vacuum from 20.0 to 24.0psi @11:00:18.32 SS.reconfigure SSinhale.with maxSpeed: 29 @11:00:18.36 SS.seek 25ml @11:00:29.57 Vacuum too high -- waiting up to 40s @11:00:31.73 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:00:31.75 SS.reconfigure SSinhale.with maxSpeed: 26 @11:00:31.80 SS.seek 25ml @11:00:41.61 Stroke completed -- waiting for pressure to equalize ... @11:00:47.99 Intake.select :close @11:00:48.15 SSV.dial :exhaust @11:00:49.00 Exhaust.select :open @11:00:49.12 SS.reconfigure SSexhale @11:00:49.17 SS.seek 0ml @11:00:49.75 delay 5 seconds @11:00:54.78 ATV.dial ASV @11:00:55.74 ASV.dial :SPEdelivery @11:00:55.76 AS.seek 0.02ml @11:00:58.44 Exhaust.select :close @11:00:58.56 SSV.dial CBV @11:00:58.58 CBV.dial SSV @11:00:59.44 Allowing 130s for 25.0ml of remaining 1450.0ml @11:00:59.45 Intake.select :open @11:01:01.64 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:01:01.66 SS.reconfigure SSinhale.with maxSpeed: 26 @11:01:01.70 SS.seek 25ml @11:01:38.12 Vacuum too high -- waiting up to 40s @11:01:40.74 Intake @31.5psia, vacuum from 20.0 to 24.0psi @11:01:40.76 SS.reconfigure SSinhale.with maxSpeed: 23 @11:01:40.81 SS.seek 25ml @11:02:15.79 Stroke completed -- waiting for pressure to equalize ... @11:02:23.56 Intake.select :close @11:02:23.73 SSV.dial :exhaust @11:02:24.58 Exhaust.select :open @11:02:24.70 SS.reconfigure SSexhale @11:02:24.75 SS.seek 0ml @11:02:33.85 Exhaust.select :close @11:02:33.97 SSV.dial CBV @11:02:33.99 CBV.dial SSV @11:02:34.86 Allowing 130s for 25.0ml of remaining 1425.0ml @11:02:34.87 Intake.select :open @11:02:37.06 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:02:37.08 SS.reconfigure SSinhale.with maxSpeed: 23 @11:02:37.12 SS.seek 25ml @11:03:53.25 Stroke completed -- waiting for pressure to equalize ... @11:04:02.21 Intake.select :close @11:04:02.37 SSV.dial :exhaust @11:04:03.22 Exhaust.select :open @11:04:03.34 SS.reconfigure SSexhale @11:04:03.40 SS.seek 0ml @11:04:12.69 Exhaust.select :close @11:04:12.81 SSV.dial CBV @11:04:12.83 CBV.dial SSV @11:04:13.10 delay 5 seconds @11:04:13.69 Allowing 130s for 25.0ml of remaining 1400.0ml @11:04:13.70 Intake.select :open @11:04:15.89 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:04:15.91 SS.reconfigure SSinhale.with maxSpeed: 23 @11:04:15.95 SS.seek 25ml @11:04:18.12 ATV.dial :air2 @11:04:19.17 AS.seek 0.52ml @11:05:32.05 Stroke completed -- waiting for pressure to equalize ... @11:05:42.17 Intake.select :close @11:05:42.33 SSV.dial :exhaust @11:05:43.80 Exhaust.select :open @11:05:44.03 SS.reconfigure SSexhale @11:05:44.08 SS.seek 0ml @11:05:53.15 Exhaust.select :close @11:05:53.27 SSV.dial CBV @11:05:53.29 CBV.dial SSV @11:05:54.15 Allowing 130s for 25.0ml of remaining 1375.0ml @11:05:54.16 Intake.select :open @11:05:56.35 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:05:56.37 SS.reconfigure SSinhale.with maxSpeed: 23 @11:05:56.41 SS.seek 25ml @11:06:00.01 delay 5 seconds @11:06:05.04 ATV.dial ASV @11:06:06.02 ASV.dial :wasteR @11:06:06.50 AS.seek 0.3ml @11:06:51.02 delay 20 seconds @11:07:05.92 Vacuum too high -- waiting up to 40s @11:07:09.09 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:07:09.11 SS.reconfigure SSinhale.with maxSpeed: 21 @11:07:09.16 SS.seek 25ml @11:07:11.05 AS.seek 0.07ml @11:07:16.51 Stroke completed -- waiting for pressure to equalize ... @11:07:24.16 Intake.select :close @11:07:24.32 SSV.dial :exhaust @11:07:25.18 Exhaust.select :open @11:07:25.30 SS.reconfigure SSexhale @11:07:25.35 SS.seek 0ml @11:07:34.33 Exhaust.select :close @11:07:34.45 SSV.dial CBV @11:07:34.47 CBV.dial SSV @11:07:35.33 Allowing 130s for 25.0ml of remaining 1350.0ml @11:07:35.34 Intake.select :open @11:07:37.53 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:07:37.54 SS.reconfigure SSinhale.with maxSpeed: 21 @11:07:37.59 SS.seek 25ml @11:07:57.55 delay 20 seconds @11:08:17.58 ATV.dial :waste2 @11:08:18.34 AS.reconfigure ASconfig @11:08:18.39 AS.seek :empty @11:08:22.77 Elution @11:08:22.78 ATV.dial :air1 @11:08:23.92 AS.reconfigure ASslow1 @11:08:23.96 AS.seek 0.049ml @11:08:34.40 delay 3 seconds @11:08:37.43 ATV.dial :ElutionWater @11:08:38.12 AS.seek 0.109ml @11:08:50.44 delay 3 seconds @11:08:53.47 ATV.dial ASV,via: :air2 @11:08:55.58 ASV.dial :SPEelution @11:08:56.18 AFV.select :carrierSolution @11:08:56.34 AS.reconfigure ASnorm @11:08:56.40 AS.seek 0.759ml @11:09:01.19 Stroke completed -- waiting for pressure to equalize ... @11:09:12.07 Intake.select :close @11:09:12.26 SSV.dial :exhaust @11:09:13.12 Exhaust.select :open @11:09:13.24 SS.reconfigure SSexhale @11:09:13.29 SS.seek 0ml @11:09:22.33 Exhaust.select :close @11:09:22.46 SSV.dial CBV @11:09:22.48 CBV.dial SSV @11:09:23.33 Allowing 130s for 25.0ml of remaining 1325.0ml @11:09:23.34 Intake.select :open @11:09:25.53 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:09:25.55 SS.reconfigure SSinhale.with maxSpeed: 21 @11:09:25.59 SS.seek 25ml @11:09:30.16 delay 3 seconds @11:09:33.19 AFV.select :holdingCoil @11:09:33.34 AS.reconfigure ASslow1 @11:09:33.40 AS.seek 0.474ml @11:10:23.07 Vacuum too high -- waiting up to 40s @11:10:26.46 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:10:26.47 SS.reconfigure SSinhale.with maxSpeed: 19 @11:10:26.53 SS.seek 25ml @11:10:31.01 delay 5 seconds @11:10:36.10 AS.reconfigure ASslow2 @11:10:36.15 AS.seek 0.439ml @11:10:55.90 Stroke completed -- waiting for pressure to equalize ... @11:11:06.79 Intake.select :close @11:11:06.96 SSV.dial :exhaust @11:11:07.82 Exhaust.select :open @11:11:07.94 SS.reconfigure SSexhale @11:11:07.99 SS.seek 0ml @11:11:11.59 delay 1:00 @11:11:17.42 Exhaust.select :close @11:11:17.54 SSV.dial CBV @11:11:17.56 CBV.dial SSV @11:11:18.41 Allowing 130s for 25.0ml of remaining 1300.0ml @11:11:18.42 Intake.select :open @11:11:20.61 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:11:20.63 SS.reconfigure SSinhale.with maxSpeed: 19 @11:11:20.67 SS.seek 25ml @11:12:11.62 pushing eluate to holding coil @11:12:11.69 AS.seek 0.279ml @11:12:43.14 Vacuum too high -- waiting up to 40s @11:12:47.09 Intake @31.2psia, vacuum from 20.0 to 24.0psi @11:12:47.11 SS.reconfigure SSinhale.with maxSpeed: 17 @11:12:47.16 SS.seek 25ml @11:12:58.54 Stroke completed -- waiting for pressure to equalize ... @11:13:09.33 Intake.select :close @11:13:09.49 SSV.dial :exhaust @11:13:10.35 Exhaust.select :open @11:13:10.47 SS.reconfigure SSexhale @11:13:10.52 SS.seek 0ml @11:13:19.65 Exhaust.select :close @11:13:19.77 SSV.dial CBV @11:13:19.79 CBV.dial SSV @11:13:20.66 Allowing 130s for 25.0ml of remaining 1275.0ml @11:13:20.67 Intake.select :open @11:13:23.49 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:13:23.62 SS.reconfigure SSinhale.with maxSpeed: 17 @11:13:23.66 SS.seek 25ml @11:14:52.79 delay 30 seconds @11:15:01.77 Vacuum too high -- waiting up to 40s @11:15:05.74 Intake @31.3psia, vacuum from 20.0 to 24.0psi @11:15:05.75 SS.reconfigure SSinhale.with maxSpeed: 15 @11:15:05.80 SS.seek 25ml @11:15:12.41 Stroke completed -- waiting for pressure to equalize ... @11:15:22.60 Intake.select :close @11:15:22.76 SSV.dial :exhaust @11:15:22.82 ATV.dial :waste,via: :air3 @11:15:23.62 Exhaust.select :open @11:15:23.74 SS.reconfigure SSexhale @11:15:23.79 SS.seek 0ml @11:15:24.51 AS.reconfigure ASconfig @11:15:24.56 AS.seek :empty @11:15:32.95 Exhaust.select :close @11:15:33.07 SSV.dial CBV @11:15:33.09 CBV.dial SSV @11:15:33.96 Allowing 130s for 25.0ml of remaining 1250.0ml @11:15:33.97 Intake.select :open @11:15:36.16 Intake @31.4psia, vacuum from 20.0 to 24.0psi @11:15:36.18 SS.reconfigure SSinhale.with maxSpeed: 15 @11:15:36.22 SS.seek 25ml @11:15:40.95 Parking Bleach in LysateSource line and SPEcolumn @11:15:40.96 ATV.dial :air3 @11:15:41.80 AS.reconfigure ASslow1 @11:15:41.85 AS.seek 0.029ml @11:15:48.17 ATV.dial :ElutionWater @11:15:48.78 AS.seek 0.059ml @11:15:55.09 ATV.dial :air3 @11:15:55.71 AS.seek 0.069ml @11:15:57.98 delay 2 seconds @11:16:00.00 ATV.dial :bleach @11:16:00.52 AS.seek 0.769ml @11:17:33.12 Stroke completed -- waiting for pressure to equalize ... @11:17:44.04 SS.reconfigure SSconfig @11:17:44.09 SS.hold @11:17:44.22 Intake.select :close @11:17:44.38 ShallowSampler::Clogged in WCR -- progress too slow after 275.0ml @11:17:44.40 SSV.dial :exhaust @11:17:45.23 Exhaust.select :open @11:17:45.35 SS.reconfigure SSexhale @11:17:45.40 SS.seek 0ml @11:17:54.74 Exhaust.select :close @11:17:54.85 Sampled 275.0ml @11:17:54.87 Drying puck before fixing for 4:00 @11:17:54.88 CBV.dial :waste2b @11:17:55.71 delay 1:00 @11:18:21.54 delay 3 seconds @11:18:24.57 ATV.dial :air3 @11:18:25.12 delay 3 seconds @11:18:28.21 AS.seek 0.794ml @11:18:33.48 delay 3 seconds @11:18:36.50 ATV.dial :bleach @11:18:37.13 AS.seek 0.779ml @11:18:40.43 delay 3 seconds @11:18:43.46 ATV.dial :AIV @11:18:44.70 AS.reconfigure ASconfig @11:18:44.75 AS.seek 0.015ml @11:18:55.74 CSV.dial :air @11:18:56.23 CS.reconfigure CSfast @11:18:56.28 CS.seek 10ml @11:19:21.68 Pumping wcr puck from tube 4 up to 55psi... @11:19:21.69 CBV.dial SSV @11:19:23.10 CBV.dial between CSV and waste2b @11:19:24.17 CSV.dial between air and CTV @11:19:24.54 CS.seek 7.194ml @11:19:28.98 ATV.dial :waste @11:19:30.44 AS.seek :empty @11:19:31.64 ATV.dial :air3 @11:19:32.33 AS.reconfigure ASslow1 @11:19:32.40 AS.seek 0.009ml @11:19:32.45 CTV.dial CSV,avoiding: :air @11:19:32.47 CSV.dial CTV @11:19:33.38 CS.reconfigure CSpressurize @11:19:33.43 CS.seek 0ml @11:19:34.83 delay 2 seconds @11:19:36.85 ATV.dial :bleach @11:19:37.30 AS.seek 0.579ml @11:19:51.92 CBV.dial :waste2b @11:19:52.28 CS.reconfigure CSconfig @11:19:52.33 CS.seek :empty @11:19:53.62 delay 1:00 @11:20:53.65 CTV.dial :air,avoiding: :intake @11:20:55.14 Fix #1 of 2 (with rnal) for 2:00 @11:20:55.16 CSV.dial CSR @11:20:56.44 Pulling 2ml of CSR.rnal @11:20:56.45 CSR.select :rnal @11:20:56.57 CS.seek 2ml @11:21:02.71 delay 5 seconds @11:21:07.74 CSR.select :air @11:21:07.87 CS.reconfigure CSfast @11:21:07.92 CS.seek 10ml @11:21:28.22 Pumping wcr puck from tube 4 up to 55psi... @11:21:28.24 CBV.dial SSV @11:21:29.63 CBV.dial between CSV and waste2b @11:21:30.71 CSV.dial between air and CTV @11:21:31.40 CS.seek 7.168ml @11:21:32.19 delay 3 seconds @11:21:35.21 ATV.dial :air3 @11:21:35.75 delay 3 seconds @11:21:38.84 AS.seek 0.604ml @11:21:39.26 CTV.dial CSV,avoiding: :air @11:21:39.28 CSV.dial CTV @11:21:40.87 CS.reconfigure CSpressurize @11:21:40.92 CS.seek 0ml @11:21:44.12 delay 3 seconds @11:21:47.15 ATV.dial :bleach @11:21:47.76 AS.seek 0.589ml @11:21:51.05 delay 3 seconds @11:21:54.08 ATV.dial :ASV @11:21:55.11 ASV.dial :wasteR @11:21:55.71 AS.seek 0ml @11:21:57.99 delay 2:00 @11:23:54.44 delay 1:00 @11:23:58.02 CBV.dial :waste2b @11:23:58.39 CS.reconfigure CSconfig @11:23:58.44 CS.seek :empty @11:24:01.19 delay 1:00 @11:24:54.47 AFV.select :carrierSolution @11:24:54.57 delay 2 seconds @11:24:56.60 AS.reconfigure ASconfig @11:24:56.65 AS.seek 1ml @11:25:01.22 CTV.dial :air,avoiding: :intake @11:25:02.70 Fix #2 of 2 (with rnal) for 2:00 @11:25:02.72 CSV.dial CSR @11:25:03.26 Pulling 2ml of CSR.rnal @11:25:03.28 CSR.select :rnal @11:25:03.40 CS.seek 2ml @11:25:09.63 delay 5 seconds @11:25:14.66 CSR.select :air @11:25:14.79 CS.reconfigure CSfast @11:25:14.84 CS.seek 10ml @11:25:35.09 Pumping wcr puck from tube 4 up to 55psi... @11:25:35.11 CBV.dial SSV @11:25:36.49 CBV.dial between CSV and waste2b @11:25:37.57 CSV.dial between air and CTV @11:25:38.25 CS.seek 7.155ml @11:25:46.11 CTV.dial CSV,avoiding: :air @11:25:46.13 CSV.dial CTV @11:25:47.70 CS.reconfigure CSpressurize @11:25:47.75 CS.seek 0ml @11:25:54.41 delay 1 second @11:25:55.43 AFV.select :holdingCoil @11:25:55.53 delay 1 second @11:25:56.56 ATV.dial ASV @11:25:56.57 ASV.dial :wasteR @11:25:56.59 AS.reconfigure ASslow1 @11:25:56.63 AS.seek 0ml @11:26:05.90 delay 2:00 @11:28:05.93 CBV.dial :waste2b @11:28:06.30 CS.reconfigure CSconfig @11:28:06.35 CS.seek :empty @11:28:08.72 delay 1:00 @11:29:08.75 CTV.dial :air,avoiding: :intake @11:29:10.29 Storing wcr puck in Collection Clamp to tube 3 @11:29:10.49 SC.reconfigure SCtight @11:29:10.54 SC.seek 3 @11:29:12.90 SC.reconfigure SCloose @11:29:13.13 Elbow.reconfigure TightElbow @11:29:13.19 Elbow.seek 3 @11:29:15.10 Forearm.reconfigure TightForearm @11:29:15.15 Forearm.seek 3 + 700 counts @11:29:16.20 SE.reconfigure SEpick @11:29:16.28 SE.seek beamBreak @11:29:18.00 delay 20 seconds @11:29:19.15 SE.configure SEconfig @11:29:19.32 Elbow.reconfigure LimpElbow @11:29:19.38 Forearm.reconfigure LooseForearm @11:29:19.43 Forearm.seek 3 @11:29:19.74 Elbow.hold @11:29:19.82 Elbow.reconfigure TightElbow @11:29:19.93 SE.seek 4.07 Pucks below Top Plate @11:29:20.44 Forearm.force -20 @11:29:20.48 Forearm.seek 3 + 334 counts @11:29:20.83 Forearm.reconfigure TightForearm @11:29:20.88 Forearm.seek 3 + 700 counts @11:29:21.19 SE.seek 5.07 Pucks below Top Plate @11:29:21.81 Forearm.reconfigure LooseForearm @11:29:21.86 Forearm.seek :retract @11:29:22.79 Elbow.seek CC @11:29:24.55 Elbow.reconfigure LooseElbow @11:29:24.66 Forearm.seek CC @11:29:25.73 Hand.close @11:29:26.48 CC.seek :open @11:29:31.02 Forearm.seek :retract @11:29:32.07 Elbow.reconfigure TightElbow @11:29:32.12 Elbow.seek 3 @11:29:34.16 Elbow.reconfigure LimpElbow @11:29:34.22 Forearm.seek 3 @11:29:35.21 Elbow.hold @11:29:35.29 Elbow.reconfigure TightElbow @11:29:35.34 Hand.open @11:29:36.24 Forearm.force -20 @11:29:36.29 Forearm.seek 3 + 344 counts @11:29:37.30 Forearm.reconfigure TightForearm @11:29:37.37 Forearm.seek 3 + 700 counts @11:29:37.76 SE.seek :down @11:29:38.02 ATV.dial :air3 @11:29:38.91 Forearm.reconfigure LooseForearm @11:29:38.96 Forearm.seek :retract @11:29:39.54 AS.seek 0.59ml @11:29:40.00 SE.coast @11:29:40.05 SC.coast @11:29:40.09 Starting finalPurge @11:29:40.11 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:29:40.19 Hand.open @11:29:40.62 Elbow.seek FlushPuck::Garage @11:29:42.63 Elbow.reconfigure LooseElbow @11:29:42.74 Forearm.seek :garage @11:29:43.76 Hand.close @11:29:44.49 Forearm.seek :retract @11:29:45.49 Elbow.reconfigure TightElbow @11:29:45.54 Elbow.seek CC @11:29:48.50 Elbow.reconfigure LooseElbow @11:29:48.60 Forearm.reconfigure TightForearm @11:29:48.65 Forearm.seek CC @11:29:49.98 CC.seek :closed @11:29:54.26 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.14 @11:29:54.34 Hand.open @11:29:55.05 Forearm.reconfigure LooseForearm @11:29:55.10 Forearm.seek :retract @11:29:56.12 Flushing the Collection Valve::Manifold @11:29:56.26 CSV.dial CSR @11:29:56.80 Pulling 5ml of CSR.flush @11:29:56.81 CSR.select :flush @11:29:56.94 CS.seek 5ml @11:30:10.76 delay 5 seconds @11:30:15.79 CSR.select :air @11:30:15.96 CS.reconfigure CSfast @11:30:16.01 CS.seek 7.501ml @11:30:23.04 CSV.dial :waste @11:30:24.60 CS.reconfigure CSconfig @11:30:24.65 CS.seek 0ml @11:30:44.85 Flushing lower manifold's waste1 port @11:30:44.99 CSV.dial :flush @11:30:45.49 CS.reconfigure CSfast @11:30:45.55 CS.seek 5ml @11:30:58.66 delay 5 seconds @11:31:03.69 CSV.dial :air @11:31:04.25 CS.seek 7ml @11:31:10.04 CSV.dial CTV @11:31:10.07
CTV.dial CSV @11:31:11.62 CBV.dial :waste1 @11:31:13.20 CS.seek 0ml @11:31:31.13 Flushing lower manifold's waste2b port @11:31:31.27 CSV.dial :flush @11:31:32.10 CS.seek 5ml @11:31:38.51 delay 3 seconds @11:31:41.54 ATV.dial :ASV @11:31:42.91 ASV.dial :wasteR @11:31:43.00 AS.seek 0.09ml @11:31:45.17
delay 5 seconds @11:31:50.20 CSV.dial :air @11:31:50.77 CS.seek 7ml @11:31:56.63 CSV.dial CTV @11:31:56.66
CTV.dial CSV @11:31:57.17 CBV.dial :waste2b @11:31:58.02 CS.seek 0ml @11:32:15.76 Flushing upper manifold @11:32:15.90 CSV.dial :flush @11:32:16.74 CS.seek 5ml @11:32:29.70 delay 5 seconds @11:32:34.73 CSV.dial :air @11:32:35.30 CS.seek 7ml @11:32:41.13 CSV.dial CBV @11:32:41.16
CBV.dial CSV @11:32:42.38 CTV.dial :air @11:32:43.85 CS.seek 0ml @11:33:01.51 delay 7 seconds @11:33:08.55 CS.seek 3ml @11:33:16.76 CSV.dial :waste @11:33:17.25 CS.seek 0ml @11:33:23.80 delay 30 seconds @11:33:25.67
CSV.dial :flush @11:33:26.18 CS.seek 3ml @11:33:34.54 delay 5 seconds @11:33:39.57 CSV.dial :air @11:33:40.13 CS.seek 5ml @11:33:46.01 CSV.dial :air @11:33:46.03 CS.seek 0ml @11:33:53.83 ATV.dial :extSrc @11:33:54.58 delay 3 seconds @11:33:57.61 ATV.dial :ElutionWater @11:33:58.72 AS.seek 0.12ml @11:33:59.08
delay 7 seconds @11:34:05.04 ATV.dial :air3 @11:34:05.67 AS.seek 0.13ml @11:34:06.11
CS.seek 3ml @11:34:07.94 delay 2 seconds @11:34:09.96 ATV.dial :bleach @11:34:10.43 AS.seek 0.23ml @11:34:14.36
CSV.dial :waste @11:34:15.19 CS.seek 0ml @11:34:23.42 Disinfecting external Intake with mfbkill @11:34:23.56 CSV.dial CSR @11:34:25.07 Pulling 8ml of CSR.mfbkill @11:34:25.08 CSR.select :mfbkill @11:34:25.21 CS.seek 8ml @11:34:30.80 delay 3 seconds @11:34:33.83 ATV.dial :air3 @11:34:34.38 delay 3 seconds @11:34:37.47 AS.seek 0.255ml @11:34:42.77 delay 3 seconds @11:34:45.54
delay 5 seconds @11:34:45.79 ATV.dial :bleach @11:34:46.42 AS.seek 0.24ml @11:34:49.69 delay 3 seconds @11:34:50.56
CSR.select :air @11:34:50.68 CS.seek 10ml @11:34:52.71 ATV.dial :ASV @11:34:53.74 ASV.dial :SPEdelivery @11:34:54.12 AFV.select :carrierSolution @11:34:54.23 delay 2 seconds @11:34:56.26 AS.reconfigure ASconfig @11:34:56.31 AS.seek 0.5ml @11:34:56.48 CSV.dial CBV @11:34:56.51
CBV.dial CSV @11:34:58.39 CTV.dial :intake @11:34:59.23 Intake.select :open @11:34:59.35 CS.seek 2ml @11:35:11.54 delay 1 second @11:35:12.56 AFV.select :holdingCoil @11:35:12.72 AS.reconfigure ASslow1 @11:35:12.77 AS.seek 0.17ml @11:35:20.70
Intake.select :close @11:35:20.81 CTV.dial :air @11:35:21.65 CS.reconfigure CSconfig @11:35:21.70 CS.seek 0ml @11:35:27.86 Flushing lower manifold's waste1 port @11:35:27.87 CSV.dial CSR @11:35:29.08 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @11:35:29.21 CS.seek 4ml @11:35:40.45 delay 5 seconds @11:35:45.48 CSR.select :air @11:35:45.61 CS.reconfigure CSfast @11:35:45.65 CS.seek 10ml @11:36:01.04 CSV.dial CTV @11:36:01.07
CTV.dial CSV @11:36:02.62 CBV.dial :waste1 @11:36:03.13 CS.seek 0ml @11:36:19.39 SPE.hold 2:46:40,95C @11:36:19.45 delay 3:00 @11:36:28.33
Flushing lower manifold's waste2b port @11:36:28.34 CSV.dial CSR @11:36:28.93 Pulling 4ml of CSR.mfbkill CSR.select :mfbkill @11:36:29.06 CS.reconfigure CSconfig @11:36:29.11 CS.seek 4ml @11:36:40.36 delay 5 seconds @11:36:45.39 CSR.select :air @11:36:45.52 CS.reconfigure CSfast @11:36:45.56 CS.seek 10ml @11:37:01.45 CSV.dial CTV @11:37:01.49
CTV.dial CSV @11:37:02.03 CBV.dial :waste2b @11:37:02.93 CS.seek 0ml @11:37:29.02 Killing upper manifold @11:37:29.03 CSV.dial CSR @11:37:29.62 Pulling 4ml of CSR.mfbkill @11:37:29.63 CSR.select :mfbkill @11:37:29.76 CS.reconfigure CSconfig @11:37:29.81 CS.seek 4ml @11:37:41.08 delay 5 seconds @11:37:46.11 CSR.select :air @11:37:46.24 CS.reconfigure CSfast @11:37:46.28 CS.seek 10ml @11:38:02.77 CSV.dial CBV @11:38:02.80
CBV.dial CSV @11:38:04.03 CTV.dial :air @11:38:05.56 CS.seek 0ml @11:38:31.58 delay 7 seconds @11:38:38.62 CS.seek 3ml @11:38:47.05 CSV.dial :waste @11:38:47.57 CS.seek 0ml @11:38:56.11 Flushing upper manifold @11:38:56.25 CSV.dial :flush @11:38:56.78 CS.seek 5ml @11:39:10.30 delay 5 seconds @11:39:15.33 CSV.dial :air @11:39:15.90 CS.seek 7ml @11:39:19.50 AFV.select :carrierSolution @11:39:19.60 delay 2 seconds @11:39:21.63 AS.reconfigure ASconfig @11:39:21.68 AS.seek 1ml @11:39:21.99 CSV.dial CBV @11:39:22.02
CBV.dial CSV @11:39:23.21 CTV.dial :air @11:39:23.23 CS.seek 0ml @11:39:41.80 delay 7 seconds @11:39:48.84 CS.seek 3ml @11:39:57.36 CSV.dial :waste @11:39:57.89 CS.seek 0ml @11:40:06.51 CSV.dial :flush @11:40:07.01 CS.seek 3ml @11:40:09.68 delay 1 second @11:40:11.32 AFV.select :holdingCoil @11:40:11.52 delay 1 second @11:40:12.55 ASV.dial :wasteD @11:40:13.61 AS.seek 0ml @11:40:15.52
delay 5 seconds @11:40:20.55 CSV.dial :air @11:40:21.24 CS.seek 5ml @11:40:27.14 CSV.dial :air @11:40:27.16 CS.seek 0ml @11:40:40.64 delay 7 seconds @11:40:47.68 CS.seek 3ml @11:40:56.14 CSV.dial :waste @11:40:56.98 CS.seek 0ml @11:41:05.43 Flushing lower manifold's waste1 port @11:41:05.57 CSV.dial :flush @11:41:06.09 CS.seek 5ml @11:41:11.41 Priming Eluate @11:41:11.42 AFV.select :carrierSolution @11:41:11.53 delay 2 seconds @11:41:13.57 AS.seek 0.5ml @11:41:19.46
delay 5 seconds @11:41:24.49 CSV.dial :air @11:41:25.06 CS.seek 7ml @11:41:31.02 CSV.dial CTV @11:41:31.06
CTV.dial CSV @11:41:32.63 CBV.dial :waste1 @11:41:33.17 CS.seek 0ml @11:41:42.57 delay 1 second @11:41:43.59 AFV.select :holdingCoil @11:41:43.69 ATV.dial ASV @11:41:43.72 ASV.dial :SPErecovery @11:41:44.32 Swish #1 of 2 @11:41:44.40 AS.reconfigure ASslow0 @11:41:44.45 AS.seek 0.6ml @11:41:51.43
Flushing lower manifold's waste2b port @11:41:51.57 CSV.dial :flush @11:41:52.42 CS.seek 5ml @11:41:54.85 AS.seek 0.5ml @11:42:05.23 Swish #2 of 2 @11:42:05.31 AS.seek 0.6ml @11:42:05.74
delay 5 seconds @11:42:10.76 CSV.dial :air @11:42:11.34 CS.seek 7ml @11:42:15.81 AS.seek 0.5ml @11:42:17.33 CSV.dial CTV @11:42:17.36
CTV.dial CSV @11:42:17.87 CBV.dial :waste2b @11:42:18.75 CS.seek 0ml @11:42:26.20 delay 3 seconds @11:42:29.29 AS.reconfigure ASslow1 @11:42:29.34 AS.seek 0.684ml @11:42:36.93
Disinfecting external Intake with kill @11:42:37.07 CSV.dial CSR @11:42:37.74 Pulling 8ml of CSR.kill @11:42:37.75 CSR.select :kill @11:42:37.88 CS.seek 8ml @11:42:58.49 delay 5 seconds @11:43:03.52 CSR.select :air @11:43:03.65 CS.seek 10ml @11:43:06.62 delay 3 seconds @11:43:09.65 CSV.dial CBV @11:43:09.68
CBV.dial CSV @11:43:09.77 Eluate primed @11:43:09.79 ATV.dial :waste2 @11:43:10.42 AS.reconfigure ASnorm @11:43:10.47 AS.seek 0ml @11:43:10.90
CTV.dial :intake @11:43:11.74 Intake.select :open @11:43:11.86 CS.seek 2ml @11:43:33.55 Intake.select :close @11:43:33.66 CTV.dial :air @11:43:34.49 CS.reconfigure CSconfig @11:43:34.54 CS.seek 0ml @11:43:40.68 CSV.dial CSR @11:43:41.90 Pulling 5ml of CSR.flush @11:43:41.91 CSR.select :flush @11:43:42.04 CS.seek 5ml @11:43:45.28 SPE.coast @11:43:45.36 ASV.dial :SPErecovery @11:43:46.11 Setting up PCR for OM -- writing /var/log/esp/OM20may2811h.pcr @11:43:46.13 Building PCR Slug from ASV with MM3 mix, PP5 primer @11:43:46.15 Priming with carrier @11:43:46.16 ATV.dial ARV @11:43:47.12 ARV.dial :carrier @11:43:47.50 AS.seek 0.5ml @11:43:55.80
delay 5 seconds @11:44:00.83 CSR.select :air @11:44:00.96 CS.seek 10ml @11:44:13.11 AFV.select :carrierSolution @11:44:13.22 delay 2 seconds @11:44:14.77
CSV.dial CBV @11:44:15.25 AS.reconfigure ASconfig @11:44:15.31 AS.seek 1ml @11:44:15.89
CS.seek 0ml @11:44:42.62 Garaging FlushPuck in Collection Clamp @11:44:42.71 Elbow.reconfigure TightElbow @11:44:42.75 Elbow.seek CC @11:44:43.04 Elbow.reconfigure LooseElbow @11:44:43.14 Forearm.seek CC @11:44:44.15 Hand.close @11:44:44.39 delay 1 second @11:44:44.77
CC.seek :open @11:44:45.42 AFV.select :holdingCoil @11:44:45.53 delay 1 second @11:44:46.56 ATV.dial :waste @11:44:48.01 AS.reconfigure ASnorm @11:44:48.06 AS.seek :empty @11:44:49.13
Forearm.seek :retract @11:44:50.13 Elbow.reconfigure TightElbow @11:44:50.18 Elbow.seek :garage @11:44:53.38 Elbow.reconfigure LooseElbow @11:44:53.48 Forearm.reconfigure TightForearm @11:44:53.53 Forearm.seek :garage @11:44:54.68 Hand.open @11:44:55.33 Forearm.reconfigure LooseForearm @11:44:55.38 Forearm.seek :retract @11:44:56.44 Starting initialPurge @11:44:56.45 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @11:44:56.53 Hand.open @11:44:56.83 Elbow.reconfigure TightElbow @11:44:56.87 Elbow.seek FlushPuck::Garage @11:44:57.14 Elbow.reconfigure LooseElbow @11:44:57.24 Forearm.seek :garage @11:44:58.22 Hand.close @11:44:59.01 Forearm.seek :retract @11:44:59.99 Elbow.reconfigure TightElbow @11:45:00.04 Elbow.seek CC @11:45:03.02 Elbow.reconfigure LooseElbow @11:45:03.13 Forearm.reconfigure TightForearm @11:45:03.17 Forearm.seek CC @11:45:04.49 CC.seek :closed @11:45:08.74 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.19 @11:45:08.81 Hand.open @11:45:09.45 Forearm.reconfigure LooseForearm @11:45:09.50 Forearm.seek :retract @11:45:10.52 Flushing the Collection Valve::Manifold @11:45:10.66 CSV.dial CSR @11:45:12.58 Pulling 5ml of CSR.flush CSR.select :flush @11:45:12.71 CS.seek 5ml @11:45:26.46 delay 5 seconds @11:45:31.49 CSR.select :air @11:45:31.67 CS.reconfigure CSfast @11:45:31.72 CS.seek 7.5ml @11:45:38.65 CSV.dial :waste @11:45:38.83 AFV.select :carrierSolution @11:45:38.94 delay 2 seconds @11:45:40.07
CS.reconfigure CSconfig @11:45:40.12 CS.seek 0ml @11:45:40.97 AS.reconfigure ASconfig @11:45:41.02 AS.seek 0.5ml @11:46:00.31
Purging external Intake @11:46:00.45 CSV.dial :flush @11:46:00.96 CS.reconfigure CSfast @11:46:01.02 CS.seek 10ml @11:46:10.11 delay 1 second @11:46:11.14 AFV.select :holdingCoil @11:46:11.24 Building Slug @11:46:11.26 ATV.dial :ARV @11:46:12.83 delay 2 seconds @11:46:14.86 ARV.dial :air1 @11:46:15.26 delay 2 seconds @11:46:17.35 AS.reconfigure ASslow2 @11:46:17.40 AS.seek 0.506ml @11:46:23.70 delay 2 seconds @11:46:25.73 ARV.dial :MM3 @11:46:25.87
delay 5 seconds @11:46:26.75 delay 2 seconds @11:46:28.78 Adding MM3 @11:46:28.87 AS.seek 0.524ml @11:46:30.89 CSV.dial CBV @11:46:30.93
CBV.dial CSV @11:46:31.75 CTV.dial :intake @11:46:32.59 Intake.select :open @11:46:32.71 CS.seek 0ml @11:46:47.38 delay 25 seconds @11:46:59.12
Intake.select :close @11:46:59.23 CTV.dial :air @11:47:00.07 Flushing upper manifold @11:47:00.21 CSV.dial :flush @11:47:01.02 CS.seek 5ml @11:47:12.41 ARV.dial :PP5 @11:47:12.81 Adding PP5 @11:47:12.83 delay 2 seconds @11:47:13.87
delay 5 seconds @11:47:14.91 AS.seek 0.53ml @11:47:18.89
CSV.dial :air @11:47:19.44 CS.seek 7ml @11:47:21.28 delay 10 seconds @11:47:25.18 CSV.dial CBV @11:47:25.20
CBV.dial CSV @11:47:26.35 CTV.dial :air @11:47:26.37 CS.seek 0ml @11:47:31.31 ARV.dial :air3 @11:47:31.70 adding ARV air @11:47:31.72 delay 2 seconds @11:47:33.80 AS.seek 0.536ml @11:47:40.09 delay 5 seconds @11:47:44.12
delay 7 seconds @11:47:45.11 ARV.dial :carrier @11:47:46.34 delay 7 seconds @11:47:51.16
CS.seek 3ml @11:47:53.43 AS.seek 0.594ml @11:47:59.31
CSV.dial :waste @11:47:59.82 CS.seek 0ml @11:48:08.02 CSV.dial :flush @11:48:08.52 CS.seek 3ml @11:48:16.62 delay 5 seconds @11:48:21.65 CSV.dial :air @11:48:22.21 CS.seek 5ml @11:48:28.00 CSV.dial :air @11:48:28.02 CS.seek 0ml @11:48:40.81 delay 7 seconds @11:48:47.85 CS.seek 3ml @11:48:52.19 delay 5 seconds @11:48:56.00
CSV.dial :waste @11:48:56.82 CS.seek 0ml @11:48:57.21 ATV.dial :ASV @11:48:57.59 delay 2 seconds @11:48:59.68 AS.seek 0.6ml @11:49:04.94
Garaging FlushPuck in Collection Clamp @11:49:05.76 Elbow.reconfigure TightElbow @11:49:05.81 Elbow.seek CC @11:49:06.07 Elbow.reconfigure LooseElbow @11:49:06.14 delay 4 seconds @11:49:06.24
Forearm.seek CC @11:49:07.20 Hand.close @11:49:07.77 CC.seek :open @11:49:10.18 ATV.dial ARV @11:49:10.73 delay 2 seconds @11:49:12.13
Forearm.seek :retract @11:49:12.76 ARV.dial :carrier @11:49:12.78 delay 2 seconds @11:49:13.11
Elbow.reconfigure TightElbow @11:49:13.16 Elbow.seek :garage @11:49:14.87 AS.seek 0.65ml @11:49:16.35
Elbow.reconfigure LooseElbow @11:49:16.45 Forearm.reconfigure TightForearm @11:49:16.50 Forearm.seek :garage @11:49:17.65 Hand.open @11:49:18.33 Forearm.reconfigure LooseForearm @11:49:18.38 Forearm.seek :retract @11:49:19.42 Completed [coreNEG] begun 10:00:27 @11:49:19.43 Preparing to sleep until Fri May 29 10:00:27 +0200 2020 @11:49:19.51 deferred until MFBPCR finish @11:49:19.53
Waiting for MFBPCR til 10:00CEST29-May-20 @11:49:19.55 Continuing at 10:00CEST29-May-20 @11:49:19.61 GoodNews.email "Then, in tube 4: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:176\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@11:49:19, 9.4C, 53% humidity, 14.4psia, 12.871V, 0.512A, 0.512A avg, 6.59W",Subject: "Waiting until 10:00CEST29-May-20" @11:50:05.39 delay 4 seconds @11:50:09.44 ATV.dial PCR @11:50:10.15 ASV.dial :waste1 @11:50:10.97 ARV.dial :air1 @11:50:11.42 Positioning Slug by 0.324ml @11:50:11.64 AS.seek 0.326ml @11:55:37.78 delay 5 seconds @11:55:42.81 ATV.dial ARV @11:55:43.57 ASV.dial :PCRlock @11:55:43.94 delay 5 seconds @11:55:48.97 Running OM Profile60C350 thermocycling @11:55:48.98 PCR.program Profile60C350 @11:55:49.10 Profile60C350 should complete in < 2:02:45 @11:55:49.11 PCR.run 2:38:26.25 @13:45:38.98 PCR.off @13:45:40.22 PCR for OM completed -- closed /var/log/esp/OM20may2811h.pcr @13:45:40.29 Cleaning PCR @13:45:40.30 AFV.select :carrierSolution @13:45:40.41 delay 2 seconds @13:45:42.44 AS.reconfigure ASconfig @13:45:43.26 AS.seek 0.5ml @13:45:53.57 delay 1 second @13:45:54.59 AFV.select :holdingCoil @13:45:54.69 ASV.dial :waste1 @13:45:55.07 ATV.dial :air3 @13:45:56.31 AS.reconfigure ASslow1 @13:45:56.36 AS.seek 0.51ml @13:45:58.60 delay 2 seconds @13:46:00.62 ATV.dial :bleach @13:46:01.22 AS.seek 0.81ml @13:47:01.84 delay 3 seconds @13:47:04.87 ATV.dial :air3 @13:47:05.40 delay 3 seconds @13:47:08.49 AS.seek 0.835ml @13:47:13.79 delay 3 seconds @13:47:16.82 ATV.dial :bleach @13:47:17.42 AS.seek 0.82ml @13:47:20.73 delay 3 seconds @13:47:23.76 ARV.dial :waste @13:47:24.35 delay 3 seconds @13:47:27.38 ATV.dial :ARV @13:47:28.25 AS.reconfigure ASconfig @13:47:28.30 AS.seek 0.47ml @13:47:48.71 AFV.select :carrierSolution @13:47:48.82 delay 2 seconds @13:47:50.86 AS.seek 1ml @13:48:21.65 delay 1 second @13:48:22.67 AFV.select :holdingCoil @13:48:22.77 delay 1 second @13:48:23.80 AS.reconfigure ASfast @13:48:23.85 AS.seek 0ml @13:48:51.18 AFV.select :carrierSolution @13:48:51.29 delay 2 seconds @13:48:53.32 AS.reconfigure ASconfig @13:48:53.37 AS.seek 1ml @13:49:51.14 delay 1 second @13:49:52.16 AFV.select :holdingCoil @13:49:52.26 delay 1 second @13:49:53.29 AS.reconfigure ASfast @13:49:53.34 AS.seek 0ml @13:50:20.70 AFV.select :carrierSolution @13:50:20.81 delay 2 seconds @13:50:22.84 AS.reconfigure ASconfig @13:50:22.89 AS.seek 1ml @13:51:20.62 delay 1 second @13:51:21.64 AFV.select :holdingCoil @13:51:21.74 delay 1 second @13:51:22.77 AS.reconfigure ASfast @13:51:22.82 AS.seek 0ml @13:51:50.15 ARV.dial between carrier and air1 @13:51:50.64 ATV.dial :air3 @13:51:51.90 AS.reconfigure ASslow1 @13:51:51.95 AS.seek 0.01ml @13:51:54.25 delay 2 seconds @13:51:56.27 ATV.dial :bleach @13:51:56.90 AS.seek 0.81ml @13:54:38.03 delay 3 seconds @13:54:41.06 ATV.dial :air3 @13:54:41.59 delay 3 seconds @13:54:44.68 AS.seek 0.835ml @13:54:49.95 delay 3 seconds @13:54:52.98 ATV.dial :bleach @13:54:53.59 AS.seek 0.82ml @13:54:56.88 delay 3 seconds @13:54:59.91 ATV.dial PCR @13:55:00.31 AS.reconfigure ASconfig @13:55:00.36 AS.seek :empty @13:55:47.83 PCR.program :bleachProfile @13:55:47.88 PCR program should complete in < 2:45 @13:55:47.89 PCR.run 10:00 @13:58:40.62 PCR.off @13:58:40.65 AFV.select :carrierSolution @13:58:40.76 delay 2 seconds @13:58:42.80 AS.seek 1ml @13:59:40.60 delay 1 second @13:59:41.62 AFV.select :holdingCoil @13:59:41.72 delay 1 second @13:59:42.75 AS.reconfigure ASfast @13:59:42.80 AS.seek 0ml @14:00:10.14 delay 3 seconds @14:00:13.17 AFV.select :carrierSolution @14:00:13.27 delay 2 seconds @14:00:15.30 AS.reconfigure ASconfig @14:00:15.36 AS.seek 1ml @14:01:13.18 delay 1 second @14:01:14.20 AFV.select :holdingCoil @14:01:14.30 delay 1 second @14:01:15.33 AS.reconfigure ASfast @14:01:15.38 AS.seek 0ml @14:01:43.49 delay 3 seconds @14:01:46.52 AFV.select :carrierSolution @14:01:46.62 delay 2 seconds @14:01:48.65 AS.reconfigure ASconfig @14:01:48.71 AS.seek 1ml @14:02:46.47 delay 1 second @14:02:47.49 AFV.select :holdingCoil @14:02:47.59 delay 1 second @14:02:48.62 AS.reconfigure ASfast @14:02:48.67 AS.seek 0ml @14:03:15.99 delay 3 seconds @14:03:19.02 AFV.select :carrierSolution @14:03:19.12 delay 2 seconds @14:03:21.15 AS.reconfigure ASconfig @14:03:21.21 AS.seek 1ml @14:04:18.98 delay 1 second @14:04:20.00 AFV.select :holdingCoil @14:04:20.10 delay 1 second @14:04:21.13 AS.reconfigure ASfast @14:04:21.18 AS.seek 0ml @14:04:48.54 delay 3 seconds @14:04:51.57 AFV.select :carrierSolution @14:04:51.67 delay 2 seconds @14:04:53.70 AS.reconfigure ASconfig @14:04:53.76 AS.seek 1ml @14:05:51.57 delay 1 second @14:05:52.59 AFV.select :holdingCoil @14:05:52.69 delay 1 second @14:05:53.72 AS.reconfigure ASnorm @14:05:53.77 AS.seek 0ml @14:06:44.50 Setting up PCR for ML -- writing /var/log/esp/ML20may2814h.pcr @14:06:44.52 Building PCR Slug from ASV with MM2 mix, PP4 primer @14:06:44.54 Priming with carrier @14:06:44.55 ATV.dial ARV @14:06:45.13 ARV.dial :carrier @14:06:45.48 AS.seek 0.5ml @14:07:11.04 AFV.select :carrierSolution @14:07:11.15 delay 2 seconds @14:07:13.18 AS.reconfigure ASconfig @14:07:13.23 AS.seek 1ml @14:07:42.23 delay 1 second @14:07:43.25 AFV.select :holdingCoil @14:07:43.35 delay 1 second @14:07:44.38 ATV.dial :waste @14:07:45.96 AS.reconfigure ASnorm @14:07:46.01 AS.seek :empty @14:08:36.74 AFV.select :carrierSolution @14:08:36.85 delay 2 seconds @14:08:38.88 AS.reconfigure ASconfig @14:08:38.93 AS.seek 0.5ml @14:09:07.99 delay 1 second @14:09:09.01 AFV.select :holdingCoil @14:09:09.11 Building Slug @14:09:09.12 ATV.dial :ARV @14:09:10.79 delay 2 seconds @14:09:12.82 ARV.dial :air1 @14:09:13.21 delay 2 seconds @14:09:15.30 AS.reconfigure ASslow2 @14:09:15.35 AS.seek 0.506ml @14:09:21.78 delay 2 seconds @14:09:23.80 ARV.dial :MM2 @14:09:25.23 delay 2 seconds @14:09:27.26 Adding MM2 @14:09:27.35 AS.seek 0.524ml @14:09:45.71 delay 25 seconds @14:10:10.74 ARV.dial :PP4 @14:10:11.12 Adding PP4 @14:10:11.14 delay 2 seconds @14:10:13.22 AS.seek 0.53ml @14:10:19.59 delay 10 seconds @14:10:29.62 ARV.dial :air3 @14:10:30.00 adding ARV air @14:10:30.02 delay 2 seconds @14:10:32.10 AS.seek 0.536ml @14:10:38.50 delay 5 seconds @14:10:43.53 ARV.dial :carrier @14:10:44.75 delay 7 seconds @14:10:51.84 AS.seek 0.594ml @14:11:50.43 delay 5 seconds @14:11:55.46 ATV.dial :ASV @14:11:55.86 delay 2 seconds @14:11:57.95 AS.seek 0.6ml @14:12:04.20 delay 4 seconds @14:12:08.23 ATV.dial ARV @14:12:08.86 delay 2 seconds @14:12:10.89 ARV.dial :carrier @14:12:10.90 delay 2 seconds @14:12:12.99 AS.seek 0.65ml @14:13:03.50 delay 4 seconds @14:13:07.53 ATV.dial PCR @14:13:08.12 ASV.dial :waste1 @14:13:08.13 ARV.dial :air1 @14:13:08.52 Positioning Slug by 0.324ml @14:13:08.61 AS.seek 0.326ml @14:18:34.48 delay 5 seconds @14:18:39.51 ATV.dial ARV @14:18:40.27 ASV.dial :PCRlock @14:18:40.66 delay 5 seconds @14:18:45.69 Running ML Profile60C350 thermocycling @14:18:45.71 PCR.program Profile60C350 @14:18:45.82 Profile60C350 should complete in < 2:02:45 @14:18:45.83 PCR.run 2:38:26.25 @16:57:13.67 PCR.abort @16:57:13.69 PCR.off @16:57:13.72 AnalyticalModule::PCR::Aborted in PCR -- Program aborted due to timeout @16:57:13.74 BadNews.email "Program aborted due to timeout",Subject: "AnalyticalModule::PCR::Aborted in PCR" @16:57:13.79 Thread::ChildDied in MFBPCR -- :PCR @16:57:13.81 Waiting to Resume from Checkpoint ":PCR" @16:57:22.16 BadNews.email ":PCR",Subject: "Thread::ChildDied in MFBPCR" @23:20:45.37 Session Begun @23:20:48.12 -> can @23:20:58.04 -> Power @23:21:16.03 -> showlog 50 @23:21:41.56 -> PCR.data @23:22:07.17 -> ESP.shutdown @23:22:07.21 Waiting for MFBPCR / Shutdown @23:22:11.70 UserAbort in brent -- @23:22:22.12 -> Clamp @23:22:46.36 -> ESP.background @23:22:46.38 NoMethodError in brent -- undefined method `background' for ESP:Module @23:24:06.60 -> Sleep.background @23:25:39.08 -> Sleep.background.first.abort @23:25:39.11 Thread::Aborted in MFBPCR -- "Aborted by brent" @23:25:39.14 Waiting to Resume from Checkpoint ":PCR" @23:25:50.25 -> Sleep.background.first.abort @23:25:50.28 Thread::Aborted in MFBPCR -- "Aborted by brent" @23:25:50.32 Waiting to Resume from Checkpoint ":PCR" @23:26:02.09 -> Sleep.background.first.exit @23:26:02.13 AS.coast @23:26:02.94 SS.coast @23:26:03.00 CC.coast @23:26:03.07 CS.coast @23:26:03.13 PC.coast @23:26:03.20 PS.coast @23:26:03.26 Forearm.coast @23:26:03.33 Elbow.coast @23:26:03.75 Gate.power :core,:OFF @23:26:03.77 Gate.power :raw,:OFF @23:26:03.80 Shutdown @23:26:03.83 GoodNews.email "MFBPCR completed",Subject: "Shutdown" @23:26:24.81 -> can @23:30:21.38 -> can @23:33:38.59 -> quit @23:33:38.61 Session Ended @10:00:27.66CEST29-May-20
Powering core bus @10:00:32.34 Gate.power :raw,:ON @10:00:33.12 SPE.coast @10:00:33.18 SPE.configure SPEconfig @10:00:33.28 AS.configure ASconfig @10:00:33.38 SS.configure SSconfig @10:00:33.48 CC.configure CCconfig @10:00:33.57 CS.configure CSconfig @10:00:33.67 PC.configure PCconfig @10:00:33.77 PS.configure PSconfig @10:00:33.89 Forearm.configure LooseForearm @10:00:33.99 Elbow.configure LooseElbow @10:00:34.12 SC.configure SCloose @10:00:34.21 SE.configure SEconfig @10:00:34.26 CH.coast @10:00:34.32 CH.configure CHconfig @10:00:34.35 PH.coast @10:00:34.41 PH.configure PHconfig @10:00:34.45 PCR.off @10:00:34.48 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:34.51 PCR.caltemp= 2.8814,0.0372,0 @10:00:34.55 All dwarf controllers running firmware version 3.75 @10:00:34.66 Valve.reset @10:00:34.83 PCR.off @10:00:34.87 PCR.pidtemp= 50,0.1,0,300,25,25 @10:00:34.90 PCR.caltemp= 2.8814,0.0372,0 @10:00:35.13 Intake.select :close @10:00:35.14 Exhaust.select :close @10:00:35.15 CTV.dial :air @10:00:35.25 PSV.dial :air @10:00:35.28 CSV.dial :waste1 @10:00:35.46 SSV.dial :spit,avoiding: :exhaust @10:00:35.49
Forearm.reconfigure LooseForearm @10:00:35.60 Forearm.home.seek :retract @10:00:35.87 PS.reconfigure PSconfig @10:00:35.93 PS.home.seek 0ml @10:00:36.36 Hand.close @10:00:36.43
Elbow.jog 1500 @10:00:37.22 CS.reconfigure CSfast @10:00:37.25 SS.reconfigure SSconfig @10:00:37.36 SS.home.seek 0ml @10:00:37.38 CS.home.seek 0ml @10:00:37.50
Elbow.reconfigure LooseElbow @10:00:37.55 Elbow.home.seek :clear @10:00:41.02 SE.reconfigure SEconfig @10:00:41.07 SE.home.seek :down @10:00:41.86 SC.reconfigure SCsloppy @10:00:41.91 SC.home.seek :initial @10:00:44.94 PS.coast @10:00:47.57
Forearm.seek :retract @10:00:47.65 Elbow.seek :hammer @10:00:48.92 Forearm.reconfigure TightForearm @10:00:48.96 Forearm.seek 5 + 350 counts @10:00:50.13 Forearm.seek :retract @10:00:51.36 PC.reconfigure PCconfig @10:00:51.37
CC.reconfigure CCconfig @10:00:51.47 PC.home.seek :open @10:00:51.50
CC.home.seek :open @10:00:55.06 Phase [stdphase] @10:00:55.07 GoodNews.email "At 10:00:54 began in tube 4: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:176\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "Started 20invasivedeployfinal phase stdphase" @10:00:55.09CEST29-May-20
===> STARTED Superphase (phase #22) <=== Everybody has a plan untill they get punched in the face - Mike Tyson @10:00:55.13 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @10:00:55.16 Starting initialPurge @10:00:55.17 Retry #1 of 10 after 10:00 @10:00:55.20
Loading FlushPuck into Collection Clamp from FlushPuck::Garage @10:00:55.27 Hand.open @10:00:56.19 Elbow.reconfigure TightElbow @10:00:56.25 Elbow.seek FlushPuck::Garage @10:00:58.20 Elbow.reconfigure LooseElbow @10:00:58.31 Forearm.reconfigure LooseForearm @10:00:58.36 Forearm.seek :garage @10:00:59.38 Hand.close @10:01:00.19 Forearm.seek :retract @10:01:01.19 Elbow.reconfigure TightElbow @10:01:01.24 Elbow.seek CC @10:01:04.19 Elbow.reconfigure LooseElbow @10:01:04.30 Forearm.reconfigure TightForearm @10:01:04.35 Forearm.seek CC @10:01:05.67 CC.seek :closed @10:01:09.95 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.24 @10:01:10.02 Hand.open @10:01:10.77 Forearm.reconfigure LooseForearm @10:01:10.82 Forearm.seek :retract @10:01:11.83 Flushing the Collection Valve::Manifold @10:01:11.97 CSV.dial CSR @10:01:13.45 Pulling 5ml of CSR.flush @10:01:13.46 CSR.select :flush @10:01:13.59 CS.reconfigure CSconfig @10:01:13.64 CS.seek 5ml @10:01:27.42 delay 5 seconds @10:01:32.45 CSR.select :air @10:01:32.62 CS.reconfigure CSfast @10:01:32.67 CS.seek 7.5ml @10:01:39.61 CSV.dial :waste @10:01:41.02 CS.reconfigure CSconfig @10:01:41.07 CS.seek 0ml @10:02:01.20 Purging external Intake @10:02:01.34 CSV.dial :flush @10:02:01.83 CS.reconfigure CSfast @10:02:01.88 CS.seek 10ml @10:02:26.77 delay 5 seconds @10:02:31.80 CSV.dial CBV @10:02:31.84
CBV.dial CSV @10:02:32.69 CTV.dial :intake @10:02:33.53 Intake.select :open @10:02:33.65 CS.seek 0ml @10:03:00.17 Intake.select :close @10:03:00.28 CTV.dial :air @10:03:01.11 Flushing upper manifold @10:03:01.24 CSV.dial :flush @10:03:02.06 CS.seek 5ml @10:03:14.92 delay 5 seconds @10:03:19.95 CSV.dial :air @10:03:20.63 CS.seek 7ml @10:03:26.36 CSV.dial CBV @10:03:26.38
CBV.dial CSV @10:03:27.50 CTV.dial :air @10:03:27.52 CS.seek 0ml @10:03:45.05 delay 7 seconds @10:03:52.09 CS.seek 3ml @10:04:00.24 CSV.dial :waste @10:04:00.73 CS.seek 0ml @10:04:08.98 CSV.dial :flush @10:04:10.15 CS.seek 3ml @10:04:18.33 delay 5 seconds @10:04:23.36 CSV.dial :air @10:04:24.04 CS.seek 5ml @10:04:29.77 CSV.dial :air @10:04:29.79 CS.seek 0ml @10:04:42.80 delay 7 seconds @10:04:49.84 CS.seek 3ml @10:04:57.96 CSV.dial :waste @10:04:58.76 CS.seek 0ml @10:05:06.83 Garaging FlushPuck in Collection Clamp @10:05:06.91 Elbow.reconfigure TightElbow @10:05:06.96 Elbow.seek CC @10:05:07.23 Elbow.reconfigure LooseElbow @10:05:07.34 Forearm.seek CC @10:05:08.32 Hand.close @10:05:08.95 CC.seek :open @10:05:13.31 Forearm.seek :retract @10:05:14.29 Elbow.reconfigure TightElbow @10:05:14.34 Elbow.seek :garage @10:05:17.60 Elbow.reconfigure LooseElbow @10:05:17.71 Forearm.reconfigure TightForearm @10:05:17.76 Forearm.seek :garage @10:05:18.90 Hand.open @10:05:19.64 Forearm.reconfigure LooseForearm @10:05:19.69 Forearm.seek :retract @10:05:20.79 Starting mfb @10:05:20.80 pcrBAC sampling at most 1500ml, wcr at most 1500ml @10:05:20.85 Loading sh1 puck from tube 4 into Collection Clamp @10:05:21.00 Readying the MFB @10:05:21.07 Homing MFB Syringe @10:05:21.08 AFV.select :holdingCoil @10:05:21.32 ATV.dial :waste2 @10:05:21.36
SC.reconfigure SCtight @10:05:21.44 SC.seek 4 @10:05:22.19 AS.reconfigure ASconfig @10:05:22.24 AS.home.seek :home @10:05:25.26
SC.reconfigure SCloose @10:05:25.49 Elbow.reconfigure TightElbow @10:05:25.54 Elbow.seek 4 @10:05:27.65 Forearm.reconfigure TightForearm @10:05:27.70 Forearm.seek 4 + 700 counts @10:05:28.78 SE.reconfigure SEpick @10:05:28.86 SE.seek beamBreak @10:05:30.58 SE.configure SEconfig @10:05:30.76 Elbow.seek 4 @10:05:30.96 Forearm.seek 4 + 300 counts @10:05:31.27 Forearm.reconfigure LooseForearm @10:05:31.32 Hand.close @10:05:31.97 SE.seek :down @10:05:32.21
Forearm.seek :retract @10:05:33.20 Elbow.seek CC @10:05:33.38 SE.coast @10:05:34.89
Elbow.reconfigure LooseElbow @10:05:35.00 Forearm.reconfigure TightForearm @10:05:35.05 Forearm.seek CC @10:05:37.15 CC.seek :closed @10:05:37.93 AFV.select :carrierSolution @10:05:38.09 delay 2 seconds @10:05:40.12 AS.seek 1ml @10:05:41.43
Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 1.99 @10:05:41.44 Hand.open @10:05:42.14 Forearm.reconfigure LooseForearm @10:05:42.19 Forearm.seek :retract @10:05:43.21 SC.coast @10:05:43.25 collectSample 1500ml w/bubble point of 55psi @10:05:43.26 Priming 7ml @10:05:43.27 Intake.select :close @10:05:43.28 Exhaust.select :close @10:05:43.30 SS.hold @10:05:43.54 Calibrating pressure and temperature sensors @10:05:43.55 CTV.dial :air @10:05:43.57 SSV.dial :spit,avoiding: :exhaust @10:05:43.59 SS.reconfigure SSfast @10:05:43.64 SS.seek 5ml @10:05:46.52 SSV.dial CBV @10:05:46.55
CBV.dial SSV @10:05:48.02 delay 3.8 seconds @10:05:51.97 SSV.dial :spit @10:05:53.42 SS.seek 0ml @10:05:56.18 SSV.dial CBV @10:05:57.62 CTV.dial :intake @10:05:58.47 SSV.dial CBV @10:05:58.49
CBV.dial SSV @10:05:58.51 Allowing 40s for 7.0ml of remaining 7.0ml @10:05:58.52 Intake.select :open @10:06:00.69 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:06:00.71 SS.reconfigure SSinhale.with maxSpeed: 150 @10:06:00.76 SS.seek 7ml @10:06:04.39 Vacuum too high -- waiting up to 40s @10:06:07.24 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:06:07.26 SS.reconfigure SSinhale.with maxSpeed: 135 @10:06:07.31 SS.seek 7ml @10:06:10.26 Vacuum too high -- waiting up to 40s @10:06:13.55 SS.reconfigure SSinhale.with maxSpeed: 122 @10:06:13.63 SS.seek 7ml @10:06:13.71 Stroke completed -- waiting for pressure to equalize ... @10:06:16.32 Intake.select :close @10:06:16.48 SSV.dial :exhaust @10:06:17.32 Exhaust.select :open @10:06:17.44 SS.reconfigure SSexhale @10:06:17.48 SS.seek 0ml @10:06:21.32 Exhaust.select :close @10:06:21.44 Sampling 1500.0ml @10:06:21.45 Intake.select :close @10:06:21.47 Exhaust.select :close @10:06:21.48 SS.reconfigure SSconfig @10:06:21.52 SS.hold @10:06:21.75 CTV.dial :intake @10:06:21.77 SSV.dial CBV @10:06:21.80
CBV.dial SSV @10:06:22.65 Allowing 130s for 25.0ml of remaining 1500.0ml @10:06:22.66 Intake.select :open @10:06:24.85 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:06:24.86 SS.reconfigure SSinhale.with maxSpeed: 122 @10:06:24.91 SS.seek 25ml @10:06:28.42 Vacuum too high -- waiting up to 40s @10:06:30.53 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:06:30.54 SS.reconfigure SSinhale.with maxSpeed: 110 @10:06:30.59 SS.seek 25ml @10:06:33.04 delay 1 second @10:06:33.49
Vacuum too high -- waiting up to 40s @10:06:34.06 AFV.select :holdingCoil @10:06:34.24 delay 1 second @10:06:35.30 ATV.dial :waste @10:06:35.60
Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:06:35.62 SS.reconfigure SSinhale.with maxSpeed: 99 @10:06:35.67 SS.seek 25ml @10:06:36.55 AS.reconfigure ASnorm @10:06:36.60 AS.home.seek :empty @10:06:38.44
Vacuum too high -- waiting up to 40s @10:06:40.57 SS.reconfigure SSinhale.with maxSpeed: 89 @10:06:40.61 SS.seek 25ml @10:06:43.42 Vacuum too high -- waiting up to 40s @10:06:45.49 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:06:45.51 SS.reconfigure SSinhale.with maxSpeed: 80 @10:06:45.56 SS.seek 25ml @10:06:48.77 Vacuum too high -- waiting up to 40s @10:06:50.66 SS.reconfigure SSinhale.with maxSpeed: 72 @10:06:50.71 SS.seek 25ml @10:06:53.61 Vacuum too high -- waiting up to 40s @10:06:55.47 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:06:55.49 SS.reconfigure SSinhale.with maxSpeed: 65 @10:06:55.53 SS.seek 25ml @10:06:58.57 Vacuum too high -- waiting up to 40s @10:07:00.40 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:07:00.42 SS.reconfigure SSinhale.with maxSpeed: 59 @10:07:00.47 SS.seek 25ml @10:07:04.01 Vacuum too high -- waiting up to 40s @10:07:05.86 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:07:05.87 SS.reconfigure SSinhale.with maxSpeed: 53 @10:07:05.92 SS.seek 25ml @10:07:10.57 Stroke completed -- waiting for pressure to equalize ... @10:07:15.67 Intake.select :close @10:07:15.83 SSV.dial :exhaust @10:07:16.66 Exhaust.select :open @10:07:16.78 SS.reconfigure SSexhale @10:07:16.82 SS.seek 0ml @10:07:25.66 Exhaust.select :close @10:07:25.77 SSV.dial CBV @10:07:25.80
CBV.dial SSV @10:07:26.66 Allowing 130s for 25.0ml of remaining 1475.0ml @10:07:26.67 Intake.select :open @10:07:28.84 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:07:28.86 SS.reconfigure SSinhale.with maxSpeed: 53 @10:07:28.91 SS.seek 25ml @10:07:29.15 ATV.dial :waste @10:07:29.16 ARV.dial between carrier and air1 @10:07:30.16 ASV.dial :waste1 @10:07:35.47
Vacuum too high -- waiting up to 40s @10:07:37.34 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:07:37.36 SS.reconfigure SSinhale.with maxSpeed: 48 @10:07:37.41 SS.seek 25ml @10:07:43.95 Vacuum too high -- waiting up to 40s @10:07:46.08 Intake @30.9psia, vacuum from 20.0 to 24.0psi @10:07:46.10 SS.reconfigure SSinhale.with maxSpeed: 43 @10:07:46.14 SS.seek 25ml @10:07:53.51 Vacuum too high -- waiting up to 40s @10:07:55.59 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:07:55.60 SS.reconfigure SSinhale.with maxSpeed: 39 @10:07:55.65 SS.seek 25ml @10:08:04.20 Vacuum too high -- waiting up to 40s @10:08:06.26 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:08:06.28 SS.reconfigure SSinhale.with maxSpeed: 35 @10:08:06.33 SS.seek 25ml @10:08:15.95 Vacuum too high -- waiting up to 40s @10:08:18.27 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:08:18.29 SS.reconfigure SSinhale.with maxSpeed: 32 @10:08:18.33 SS.seek 25ml @10:08:26.37 Stroke completed -- waiting for pressure to equalize ... @10:08:33.45 Intake.select :close @10:08:33.61 SSV.dial :exhaust @10:08:34.44 Exhaust.select :open @10:08:34.56 SS.reconfigure SSexhale @10:08:34.60 SS.seek 0ml @10:08:43.55 Exhaust.select :close @10:08:43.67 SSV.dial CBV @10:08:43.69
CBV.dial SSV @10:08:44.54 Allowing 130s for 25.0ml of remaining 1450.0ml @10:08:44.55 Intake.select :open @10:08:46.72 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:08:46.74 SS.reconfigure SSinhale.with maxSpeed: 32 @10:08:46.79 SS.seek 25ml @10:08:58.26 Vacuum too high -- waiting up to 40s @10:09:00.39 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:09:00.41 SS.reconfigure SSinhale.with maxSpeed: 29 @10:09:00.46 SS.seek 25ml @10:09:13.17 Vacuum too high -- waiting up to 40s @10:09:15.75 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:09:15.76 SS.reconfigure SSinhale.with maxSpeed: 26 @10:09:15.81 SS.seek 25ml @10:09:33.08 Vacuum too high -- waiting up to 40s @10:09:35.67 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:09:35.68 SS.reconfigure SSinhale.with maxSpeed: 23 @10:09:35.73 SS.seek 25ml @10:10:02.33 Stroke completed -- waiting for pressure to equalize ... @10:10:11.89 Intake.select :close @10:10:12.05 SSV.dial :exhaust @10:10:12.88 Exhaust.select :open @10:10:13.00 SS.reconfigure SSexhale @10:10:13.05 SS.seek 0ml @10:10:21.91 Exhaust.select :close @10:10:22.03 SSV.dial CBV @10:10:22.05
CBV.dial SSV @10:10:22.91 Allowing 130s for 25.0ml of remaining 1425.0ml @10:10:22.92 Intake.select :open @10:10:25.10 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:10:25.11 SS.reconfigure SSinhale.with maxSpeed: 23 @10:10:25.16 SS.seek 25ml @10:10:50.92 Vacuum too high -- waiting up to 40s @10:10:53.80 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:10:53.81 SS.reconfigure SSinhale.with maxSpeed: 21 @10:10:53.86 SS.seek 25ml @10:10:55.20 GoodNews.email "At 10:00:54 began in tube 4: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:176\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "Started 20invasivedeployfinal phase stdphase" @10:10:55.23 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @10:10:55.25 Retry #2 of 10 after 10:00 @10:11:23.67
Vacuum too high -- waiting up to 40s @10:11:26.77 Intake @31.1psia, vacuum from 20.0 to 24.0psi @10:11:26.78 SS.reconfigure SSinhale.with maxSpeed: 19 @10:11:26.83 SS.seek 25ml @10:11:55.87 Stroke completed -- waiting for pressure to equalize ... @10:12:05.37 Intake.select :close @10:12:05.53 SSV.dial :exhaust @10:12:06.37 Exhaust.select :open @10:12:06.50 SS.reconfigure SSexhale @10:12:06.54 SS.seek 0ml @10:12:15.60 Exhaust.select :close @10:12:15.72 SSV.dial CBV @10:12:15.74
CBV.dial SSV @10:12:16.59 Allowing 130s for 25.0ml of remaining 1400.0ml @10:12:16.60 Intake.select :open @10:12:18.78 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:12:18.79 SS.reconfigure SSinhale.with maxSpeed: 19 @10:12:18.84 SS.seek 25ml @10:13:48.36 Vacuum too high -- waiting up to 40s @10:13:51.50 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:13:51.52 SS.reconfigure SSinhale.with maxSpeed: 17 @10:13:51.57 SS.seek 25ml @10:13:55.00 Stroke completed -- waiting for pressure to equalize ... @10:14:03.23 Intake.select :close @10:14:03.38 SSV.dial :exhaust @10:14:04.21 Exhaust.select :open @10:14:04.33 SS.reconfigure SSexhale @10:14:04.37 SS.seek 0ml @10:14:13.32 Exhaust.select :close @10:14:13.44 SSV.dial CBV @10:14:13.46
CBV.dial SSV @10:14:14.32 Allowing 130s for 25.0ml of remaining 1375.0ml @10:14:14.33 Intake.select :open @10:14:16.51 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:14:16.52 SS.reconfigure SSinhale.with maxSpeed: 17 @10:14:16.57 SS.seek 25ml @10:15:59.56 Stroke completed -- waiting for pressure to equalize ... @10:16:09.81 Intake.select :close @10:16:09.97 SSV.dial :exhaust @10:16:10.82 Exhaust.select :open @10:16:10.94 SS.reconfigure SSexhale @10:16:10.98 SS.seek 0ml @10:16:20.26 Exhaust.select :close @10:16:20.38 SSV.dial CBV @10:16:20.40
CBV.dial SSV @10:16:21.27 Allowing 130s for 25.0ml of remaining 1350.0ml @10:16:21.28 Intake.select :open @10:16:23.46 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:16:23.47 SS.reconfigure SSinhale.with maxSpeed: 17 @10:16:23.52 SS.seek 25ml @10:18:06.49 Stroke completed -- waiting for pressure to equalize ... @10:18:16.64 Intake.select :close @10:18:16.80 SSV.dial :exhaust @10:18:17.63 Exhaust.select :open @10:18:17.75 SS.reconfigure SSexhale @10:18:17.79 SS.seek 0ml @10:18:26.87 Exhaust.select :close @10:18:26.99 SSV.dial CBV @10:18:27.01
CBV.dial SSV @10:18:27.87 Allowing 130s for 25.0ml of remaining 1325.0ml @10:18:27.88 Intake.select :open @10:18:30.05 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:18:30.07 SS.reconfigure SSinhale.with maxSpeed: 17 @10:18:30.11 SS.seek 25ml @10:20:13.35 Stroke completed -- waiting for pressure to equalize ... @10:20:25.87 Intake.select :close @10:20:26.13 SSV.dial :exhaust @10:20:26.98 Exhaust.select :open @10:20:27.10 SS.reconfigure SSexhale @10:20:27.14 SS.seek 0ml @10:20:36.71 Exhaust.select :close @10:20:36.82 SSV.dial CBV @10:20:36.86
CBV.dial SSV @10:20:37.71 Allowing 130s for 25.0ml of remaining 1300.0ml @10:20:37.72 Intake.select :open @10:20:39.90 Intake @31.3psia, vacuum from 20.0 to 24.0psi @10:20:39.91 SS.reconfigure SSinhale.with maxSpeed: 17 @10:20:39.97 SS.seek 25ml @10:20:55.27 GoodNews.email "At 10:00:54 began in tube 4: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:176\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "Started 20invasivedeployfinal phase stdphase" @10:20:55.30 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @10:20:55.32 Retry #3 of 10 after 10:00 @10:21:59.23
Vacuum too high -- waiting up to 40s @10:22:02.38 Intake @31.0psia, vacuum from 20.0 to 24.0psi @10:22:02.40 SS.reconfigure SSinhale.with maxSpeed: 15 @10:22:02.45 SS.seek 25ml @10:22:29.35 Stroke completed -- waiting for pressure to equalize ... @10:22:40.68 Intake.select :close @10:22:40.84 SSV.dial :exhaust @10:22:41.68 Exhaust.select :open @10:22:41.80 SS.reconfigure SSexhale @10:22:41.84 SS.seek 0ml @10:22:51.28 Exhaust.select :close @10:22:51.40 SSV.dial CBV @10:22:51.42
CBV.dial SSV @10:22:52.30 Allowing 130s for 25.0ml of remaining 1275.0ml @10:22:52.33 Intake.select :open @10:22:54.55 Intake @31.2psia, vacuum from 20.0 to 24.0psi @10:22:54.56 SS.reconfigure SSinhale.with maxSpeed: 15 @10:22:54.61 SS.seek 25ml @10:24:51.64 Stroke completed -- waiting for pressure to equalize ... @10:25:02.40 SS.reconfigure SSconfig @10:25:02.46 SS.hold @10:25:02.58 Intake.select :close @10:25:02.74 ShallowSampler::Clogged in MAIN -- progress too slow after 250.0ml @10:25:02.76 SSV.dial :exhaust @10:25:03.61 Exhaust.select :open @10:25:03.73 SS.reconfigure SSexhale @10:25:03.77 SS.seek 0ml @10:25:13.18 Exhaust.select :close @10:25:13.29 Sampled 250.0ml @10:25:13.31 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:25:13.32 Intake.select :close @10:25:13.39 CSV.dial :waste @10:25:13.45 CS.seek 0ml @10:25:13.56 CSV.dial :air @10:25:13.60 SSV.dial CBV,avoiding: :exhaust @10:25:13.62
CBV.dial SSV @10:25:14.59 CS.seek 10ml @10:25:15.71
CTV.dial :air @10:25:16.54 delay 15 seconds @10:25:31.57 SSV.dial :spit,avoiding: :exhaust @10:25:33.02 CTV.dial CSV,avoiding: :intake @10:25:33.07
SS.seek 0ml @10:25:33.19 SSV.dial CBV,avoiding: :exhaust @10:25:40.08 CBV.dial between SSV and waste2 @10:25:40.51 CTV.dial CSV,avoiding: :air @10:25:40.53
CSV.dial CTV @10:25:41.04 CS.reconfigure CSpressurize @10:25:41.09 CS.seek 0ml @10:26:07.16 BubblePoint: Intake @70.4psia, Outlet @14.7psia @10:26:07.17 CBV.dial SSV @10:26:07.51 SS.reconfigure SSconfig @10:26:07.57 SS.seek 10ml @10:26:13.11 delay 15 seconds @10:26:28.26 Evacuated: Intake @37.2psia, Outlet @1.2psia @10:26:28.27 SSV.dial :spit,avoiding: :exhaust @10:26:29.73 SS.reconfigure SSexhale @10:26:29.78 SS.seek 0ml @10:26:34.90 SS.reconfigure SSfast @10:26:34.95 SS.seek 5ml @10:26:37.83 SSV.dial CBV,avoiding: :exhaust @10:26:37.85
CBV.dial SSV @10:26:39.31 CTV.dial :air,avoiding: :intake @10:26:40.78 CSV.dial CTV @10:26:40.83
SS.seek 10ml @10:26:41.83 CSV.dial :air @10:26:42.33 CS.reconfigure CSfast @10:26:42.38 CS.seek 0ml @10:26:42.95 CS.coast @10:26:43.48
SSV.dial :spit,avoiding: :exhaust @10:26:44.93 SS.reconfigure SSexhale @10:26:44.98 SS.seek 0ml @10:26:49.90 SS.reconfigure SSfast @10:26:49.95 SS.seek 5ml @10:26:53.51 SSV.dial CBV,avoiding: :exhaust @10:26:53.53
CBV.dial SSV @10:26:56.01 SSV.dial :spit,avoiding: :exhaust @10:26:57.45 SS.reconfigure SSconfig @10:26:57.50 SS.seek 0ml @10:27:00.75 SS.coast @10:27:00.79 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @10:27:00.80 Intake.select :close @10:27:00.89 CSV.dial :waste @10:27:01.02 SSV.dial CBV,avoiding: :exhaust @10:27:01.06
CBV.dial SSV @10:27:01.76 CS.seek 0ml @10:27:01.83 CSV.dial :air @10:27:02.51
CTV.dial :air @10:27:02.53 delay 15 seconds @10:27:02.65 CS.seek 10ml @10:27:17.55
SSV.dial :spit,avoiding: :exhaust @10:27:19.01 CTV.dial CSV,avoiding: :intake @10:27:19.05
SS.reconfigure SSexhale @10:27:19.15 SS.seek 0ml @10:27:19.23 SSV.dial CBV,avoiding: :exhaust @10:27:27.77 CBV.dial between SSV and waste2 @10:27:28.19 CSV.dial between air and CTV @10:27:28.53 CS.seek 8.199ml @10:27:33.81 CTV.dial CSV,avoiding: :air @10:27:33.83
CSV.dial CTV @10:27:34.18 CS.reconfigure CSpressurize @10:27:34.22 CS.seek 0ml @10:27:55.83 CSV.dial :air @10:27:56.34 CS.reconfigure CSfast @10:27:56.39 CS.seek 10ml @10:28:21.11 CSV.dial between air and CTV @10:28:21.46 CS.seek 3.048ml @10:28:38.96 CTV.dial CSV,avoiding: :air @10:28:38.98
CSV.dial CTV @10:28:39.32 CS.reconfigure CSpressurize @10:28:39.36 CS.seek 0ml @10:28:46.76 BubblePoint: Intake @69.5psia, Outlet @14.1psia @10:28:46.77 CBV.dial SSV @10:28:47.13 SS.reconfigure SSconfig @10:28:47.19 SS.seek 10ml @10:28:52.61 delay 15 seconds @10:29:07.75 Evacuated: Intake @40.6psia, Outlet @15.0psia @10:29:07.77 SSV.dial :spit,avoiding: :exhaust @10:29:09.22 SS.reconfigure SSexhale @10:29:09.27 SS.seek 0ml @10:29:14.24 SS.reconfigure SSfast @10:29:14.29 SS.seek 5ml @10:29:16.98 SSV.dial CBV,avoiding: :exhaust @10:29:17.02
CBV.dial SSV @10:29:18.48 CTV.dial :air,avoiding: :intake @10:29:19.94 CSV.dial CTV @10:29:19.99
SS.seek 10ml @10:29:20.99 CSV.dial :air @10:29:21.49 CS.reconfigure CSfast @10:29:21.54 CS.seek 0ml @10:29:22.65
SSV.dial :spit,avoiding: :exhaust @10:29:23.55 CS.coast @10:29:24.79
SS.reconfigure SSexhale @10:29:24.83 SS.seek 0ml @10:29:29.87 SS.reconfigure SSfast @10:29:29.92 SS.seek 5ml @10:29:32.65 SSV.dial CBV,avoiding: :exhaust @10:29:32.67
CBV.dial SSV @10:29:35.15 SSV.dial :spit,avoiding: :exhaust @10:29:36.59 SS.reconfigure SSconfig @10:29:36.64 SS.seek 0ml @10:29:39.81 SS.coast @10:29:39.85 Lysing: Adding 1.5ml lysis @10:29:39.86 CBV.dial between CSV and waste1,avoiding: :DBV @10:29:41.50 CTV.dial CSV @10:29:42.97 CSV.dial CSR @10:29:43.84 Pulling 1ml of CSR.lysis @10:29:43.85 CSR.select :lysis @10:29:43.97 CS.reconfigure CSconfig @10:29:44.01 CS.seek 1ml @10:29:47.52 delay 5 seconds @10:29:52.55 CSR.select :air @10:29:52.73 CS.seek 6ml @10:30:06.46 delay 2 seconds @10:30:08.48 CSV.dial :waste1 @10:30:09.89 CS.reconfigure CSfast @10:30:09.94 CS.seek :empty @10:30:25.24 CSV.dial CSR @10:30:26.72 Pulling 1.5ml of CSR.lysis @10:30:26.73 CSR.select :lysis @10:30:26.86 CS.reconfigure CSconfig @10:30:26.91 CS.seek 1.5ml @10:30:31.74 delay 5 seconds @10:30:36.77 CSR.select :air @10:30:36.95 CS.seek 4.5ml @10:30:45.55 delay 2 seconds @10:30:47.57 CSV.dial CTV @10:30:48.09 CS.seek 1.275ml @10:30:55.34 GoodNews.email "At 10:00:54 began in tube 4: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:176\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "Started 20invasivedeployfinal phase stdphase" @10:30:55.37 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @10:30:55.39 Retry #4 of 10 after 10:00 @10:30:57.31
Heating sh1 puck from tube 4 in Collection Clamp to 85C for 5:00 @10:30:57.33 CH.seek 85C,8:00 @10:34:42.91 CH.hold 5:00,85C @10:39:43.07 Collection Puck Heater at 85C after lysing @10:39:43.09 CS.seek 0ml @10:39:47.35 CTV.dial between spare and air,avoiding: :intake @10:39:48.50 CSV.dial :air @10:39:48.99 CS.seek 5ml @10:40:02.72 CSV.dial CTV @10:40:03.22 delay 2 seconds @10:40:05.24 CSV.dial :air @10:40:05.86 delay 2 seconds @10:40:07.88 CSV.dial CTV @10:40:08.39 delay 2 seconds @10:40:10.41 CSV.dial :air @10:40:10.92 delay 2 seconds @10:40:12.95 CS.seek 0ml @10:40:26.69 Recovering Lysate into Collection syringe @10:40:26.70 CSV.dial CBV @10:40:27.81 CBV.dial CSV @10:40:28.23 CS.seek 5ml @10:40:41.97 delay 5 seconds @10:40:47.00 CTV.dial :air @10:40:47.57 CS.seek 7ml @10:40:53.63 delay 5 seconds @10:40:55.41 GoodNews.email "At 10:00:54 began in tube 4: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:176\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "Started 20invasivedeployfinal phase stdphase" @10:40:55.44 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @10:40:55.46 Retry #5 of 10 after 10:00 @10:40:58.65
CSV.dial :air2 @10:40:59.15 CS.seek 10ml @10:41:07.75 CSV.dial between air and CTV,avoiding: CBV @10:41:09.02 Lysing Complete @10:41:09.10 Storing sh1 puck in Collection Clamp to tube 3 @10:41:09.29 SC.reconfigure SCtight @10:41:10.05 SC.seek 3 @10:41:12.19 SC.reconfigure SCloose @10:41:12.42 Elbow.reconfigure TightElbow @10:41:12.47 Elbow.seek 3 @10:41:14.40 Forearm.reconfigure TightForearm @10:41:14.45 Forearm.seek 3 + 700 counts @10:41:15.48 SE.reconfigure SEpick @10:41:15.56 SE.seek beamBreak @10:41:18.19 SE.configure SEconfig @10:41:18.36 Elbow.reconfigure LimpElbow @10:41:18.41 Forearm.reconfigure LooseForearm @10:41:18.46 Forearm.seek 3 @10:41:18.73 Elbow.hold @10:41:18.81 Elbow.reconfigure TightElbow @10:41:18.91 SE.seek 5.07 Pucks below Top Plate @10:41:19.44 Forearm.force -20 @10:41:19.48 Forearm.seek 3 + 338 counts @10:41:19.80 Forearm.reconfigure TightForearm @10:41:19.85 Forearm.seek 3 + 700 counts @10:41:20.14 SE.seek 6.07 Pucks below Top Plate @10:41:20.75 Forearm.reconfigure LooseForearm @10:41:20.80 Forearm.seek :retract @10:41:21.70 Elbow.seek CC @10:41:23.44 Elbow.reconfigure LooseElbow @10:41:23.54 Forearm.seek CC @10:41:26.55 Slide::Error in MAIN -- Forearm trajectoryErr at CC @10:41:26.56 Retry #1 of 3 @10:41:26.65 Elbow.reconfigure TightElbow @10:41:26.70 Elbow.seek CC @10:41:26.88 Elbow.reconfigure LooseElbow @10:41:27.00 Forearm.seek CC @10:41:29.25 Slide::Error in MAIN -- Forearm trajectoryErr at CC @10:41:29.26 Retry #2 of 3 @10:41:29.35 Elbow.reconfigure TightElbow @10:41:29.40 Elbow.seek CC @10:41:29.59 Elbow.reconfigure LooseElbow @10:41:29.69 Forearm.seek CC @10:41:31.95 Slide::Error in MAIN -- Forearm trajectoryErr at CC @10:41:31.96 Retry #3 of 3 @10:41:32.05 Elbow.reconfigure TightElbow @10:41:32.10 Elbow.seek CC @10:41:32.27 Elbow.reconfigure LooseElbow @10:41:32.39 Forearm.seek CC @10:41:34.65 SE.seek :down @10:41:36.86 SE.coast @10:41:36.98 Forearm.seek :retract @10:41:37.93 Slide::Error in MAIN -- Forearm trajectoryErr at CC @10:41:37.94 Waiting to Resume from Checkpoint "Forearm trajectoryErr at CC" @10:50:55.48 GoodNews.email "At 10:00:54 began in tube 4: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:176\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "Started 20invasivedeployfinal phase stdphase" @10:50:55.51 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @10:50:55.53 Retry #6 of 10 after 10:00 @11:00:55.55 GoodNews.email "At 10:00:54 began in tube 4: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:176\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "Started 20invasivedeployfinal phase stdphase" @11:00:55.58 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @11:00:55.59 Retry #7 of 10 after 10:00 @11:10:55.62 GoodNews.email "At 10:00:54 began in tube 4: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:176\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "Started 20invasivedeployfinal phase stdphase" @11:10:55.68 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @11:10:55.70 Retry #8 of 10 after 10:00 @11:20:55.72 GoodNews.email "At 10:00:54 began in tube 4: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:176\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "Started 20invasivedeployfinal phase stdphase" @11:20:55.75 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @11:20:56.47 Retry #9 of 10 after 10:00 @11:30:56.50 GoodNews.email "At 10:00:54 began in tube 4: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:176\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "Started 20invasivedeployfinal phase stdphase" @11:30:56.53 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @11:30:56.55 Retry #10 of 10 after 10:00 @11:40:56.57 GoodNews.email "At 10:00:54 began in tube 4: stdphase\n/home/esp/esp2/mission/20invasivedeployfinal.rb:176\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "Started 20invasivedeployfinal phase stdphase" @11:40:56.60 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @11:40:56.61 BACfans.email "Started mfb in tube 4 at 10:05:20CEST29-May-20\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @11:40:56.64 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @11:40:56.66 Retry #1 of 10 after 10:00 @11:50:56.68 BACfans.email "Started mfb in tube 4 at 10:05:20CEST29-May-20\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @11:50:56.71 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @11:50:56.73 Retry #2 of 10 after 10:00 @12:00:56.75 BACfans.email "Started mfb in tube 4 at 10:05:20CEST29-May-20\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @12:00:56.78 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @12:00:56.80 Retry #3 of 10 after 10:00 @12:10:56.82 BACfans.email "Started mfb in tube 4 at 10:05:20CEST29-May-20\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @12:10:56.85 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @12:10:56.87 Retry #4 of 10 after 10:00 @12:20:56.89 BACfans.email "Started mfb in tube 4 at 10:05:20CEST29-May-20\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @12:20:56.92 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @12:20:56.94 Retry #5 of 10 after 10:00 @12:30:56.96 BACfans.email "Started mfb in tube 4 at 10:05:20CEST29-May-20\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @12:30:56.99 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @12:30:57.01 Retry #6 of 10 after 10:00 @12:40:57.03 BACfans.email "Started mfb in tube 4 at 10:05:20CEST29-May-20\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @12:40:57.06 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @12:40:57.08 Retry #7 of 10 after 10:00 @12:50:57.10 BACfans.email "Started mfb in tube 4 at 10:05:20CEST29-May-20\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @12:50:57.13 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @12:50:57.15 Retry #8 of 10 after 10:00 @13:00:57.17 BACfans.email "Started mfb in tube 4 at 10:05:20CEST29-May-20\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @13:00:57.20 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @13:00:57.22 Retry #9 of 10 after 10:00 @13:10:57.24 BACfans.email "Started mfb in tube 4 at 10:05:20CEST29-May-20\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @13:10:57.27 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @13:10:57.29 Retry #10 of 10 after 10:00 @13:20:57.31 BACfans.email "Started mfb in tube 4 at 10:05:20CEST29-May-20\n{1 => 22, 2 => 22, 3 => 6, 4 => 16}\nCan@10:00:55, 9.0C, 52% humidity, 14.3psia, 12.915V, 0.802A, 0.802A avg, 10.36W",Subject: "pcrBAC sampling at most 1500ml, wcr at most 1500ml" @13:20:57.34 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @13:20:57.35 BadNews.email "Forearm trajectoryErr at CC",Subject: "Slide::Error in MAIN" @13:20:57.38 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @13:20:57.40 Retry #1 of 10 after 10:00 @13:30:57.42 BadNews.email "Forearm trajectoryErr at CC",Subject: "Slide::Error in MAIN" @13:30:57.45 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @13:30:57.46 Retry #2 of 10 after 10:00 @13:40:57.49 BadNews.email "Forearm trajectoryErr at CC",Subject: "Slide::Error in MAIN" @13:40:57.52 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @13:40:57.53 Retry #3 of 10 after 10:00 @13:50:57.56 BadNews.email "Forearm trajectoryErr at CC",Subject: "Slide::Error in MAIN" @13:50:57.59 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @13:50:57.60 Retry #4 of 10 after 10:00 @14:00:57.63 BadNews.email "Forearm trajectoryErr at CC",Subject: "Slide::Error in MAIN" @14:00:57.66 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @14:00:57.68 Retry #5 of 10 after 10:00 @14:10:57.70 BadNews.email "Forearm trajectoryErr at CC",Subject: "Slide::Error in MAIN" @14:10:57.73 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @14:10:57.74 Retry #6 of 10 after 10:00 @14:20:57.77 BadNews.email "Forearm trajectoryErr at CC",Subject: "Slide::Error in MAIN" @14:20:57.80 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @14:20:57.82 Retry #7 of 10 after 10:00 @14:30:57.84 BadNews.email "Forearm trajectoryErr at CC",Subject: "Slide::Error in MAIN" @14:30:57.87 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @14:30:57.88 Retry #8 of 10 after 10:00 @14:40:57.91 BadNews.email "Forearm trajectoryErr at CC",Subject: "Slide::Error in MAIN" @14:40:57.94 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @14:40:57.95 Retry #9 of 10 after 10:00 @14:50:57.98 BadNews.email "Forearm trajectoryErr at CC",Subject: "Slide::Error in MAIN" @14:50:58.01 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @14:50:58.02 Retry #10 of 10 after 10:00 @15:00:58.05 BadNews.email "Forearm trajectoryErr at CC",Subject: "Slide::Error in MAIN" @15:00:58.08 Errno::ECONNREFUSED in Qmail -- Connection refused - connect(2) @23:08:58.08CEST23-Jun-20 Slide::Error in MAIN -- Collection Clamp positionErr at closed - 203 counts @23:08:58.11
Waiting to Resume from Checkpoint "Forearm trajectoryErr at CC" @14:26:48.28CEST25-Jun-20 Slide::Error in MAIN -- Forearm positionErr at retract - 402 counts @14:26:48.29 Waiting to Resume from Checkpoint "Forearm trajectoryErr at CC" @16:34:38.60 Session Begun @16:34:42.28 -> showlog 100 @16:35:56.81 -> can @16:36:24.03 -> exit @16:36:24.04 Session Ended @17:44:19.17 Session Begun @17:44:23.10 -> showlog 1000 @17:46:34.00 -> exit @17:46:34.01 Session Ended @15:01:27.18CEST13-Jul-20
ESP2 v3.30 I2C Gateway v3.06 for core bus @15:01:29.17 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @15:01:29.48 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @15:01:35.02 Polling Can every 10:00 @15:01:36.91 SPE.coast @15:01:36.97 SPE.configure SPEconfig @15:01:37.07 AS.configure ASconfig @15:01:37.17 SS.reconfigure SSconfig @15:01:37.21 SS.hold @15:01:37.27 SS.configure SSconfig @15:01:37.37 CC.reconfigure CCconfig @15:01:37.41 CC.hold @15:01:37.48 CC.configure CCconfig @15:01:37.57 CS.reconfigure CSconfig @15:01:37.61 CS.hold @15:01:37.66 CS.configure CSconfig @15:01:37.76 PC.reconfigure PCconfig @15:01:37.80 PC.hold @15:01:38.27 PC.configure PCconfig @15:01:38.37 PS.configure PSconfig @15:01:38.49 Forearm.reconfigure LooseForearm @15:01:38.54 Forearm.hold @15:01:38.60 Forearm.configure LooseForearm @15:01:38.69 Elbow.reconfigure LooseElbow @15:01:38.73 Elbow.hold @15:01:38.78 Elbow.configure LooseElbow @15:01:38.90 SC.reconfigure SCloose @15:01:38.94 SC.hold @15:01:39.01 SC.configure SCloose @15:01:39.11 SE.reconfigure SEconfig @15:01:39.15 SE.hold @15:01:39.22 SE.configure SEconfig @15:01:39.26 CH.coast @15:01:39.32 CH.configure CHconfig @15:01:39.35 PH.coast @15:01:39.41 PH.configure PHconfig @15:01:39.45 PCR.off @15:01:39.47 PCR.pidtemp= 50,0.1,0,300,25,25 @15:01:39.50 PCR.caltemp= 2.8814,0.0372,0 @15:01:39.53 All dwarf controllers running firmware version 3.75 @15:01:39.67 Loading /home/esp/esp2/mission/InvasivePostCoreNeg.rb mission @15:01:39.74 GoodNews.email "Starting InvasivePostCoreNeg w/pucks in tube 2\n{}\nCan@15:01:35, 22.4C, 44% humidity, 14.7psia, 11.977V, 0.758A, 0.758A avg, 9.08W",Subject: "InvasivePostCoreNeg begun" @15:01:39.79
Preparing to sleep until +00:01:00 @15:01:39.85 SS.coast @15:01:39.88
Asleep til +00:01:00 @15:01:39.91 Continuing at 15:02 @15:01:39.94 CC.coast @15:01:40.02 CS.coast @15:01:40.07 PC.coast @15:01:40.11 Forearm.coast @15:01:40.14 Elbow.coast @15:01:40.18 SC.coast @15:01:40.22 SE.coast @15:01:40.62 Gate.power :core,:OFF @15:01:40.64 Gate.power :raw,:OFF @15:01:40.65 Shutdown @15:01:45.52 Session Begun @15:01:48.77 GoodNews.email "Then, in tube 2: coreNEG\n/home/esp/esp2/mission/InvasivePostCoreNeg.rb:113\n{}\nCan@15:01:39, 22.3C, 45% humidity, 14.7psia, 11.962V, 0.796A, 0.796A avg, 9.52W",Subject: "Asleep until 15:02" @15:01:51.90 -> exit @15:01:51.96 Session Ended @15:02:39.84
Powering core bus @15:02:45.10 Gate.power :raw,:ON @15:02:47.33 SPE.coast @15:02:47.54 SPE.configure SPEconfig @15:02:47.87 AS.configure ASconfig @15:02:48.20 SS.configure SSconfig @15:02:48.56 CC.configure CCconfig @15:02:48.91 CS.configure CSconfig @15:02:49.28 PC.configure PCconfig @15:02:49.64 PS.configure PSconfig @15:02:50.16 Forearm.configure LooseForearm @15:02:50.60 Elbow.configure LooseElbow @15:02:51.10 SC.configure SCloose @15:02:51.41 SE.configure SEconfig @15:02:51.53 CH.coast @15:02:51.73 CH.configure CHconfig @15:02:51.85 PH.coast @15:02:52.06 PH.configure PHconfig @15:02:52.18 PCR.off @15:02:52.27 PCR.pidtemp= 50,0.1,0,300,25,25 @15:02:52.38 PCR.caltemp= 2.8814,0.0372,0 @15:02:52.45 All dwarf controllers running firmware version 3.75 @15:02:52.67 Valve.reset @15:02:53.13 PCR.off @15:02:53.19 PCR.pidtemp= 50,0.1,0,300,25,25 @15:02:53.28 PCR.caltemp= 2.8814,0.0372,0 @15:02:53.87 Intake.select :close @15:02:54.13 Exhaust.select :close @15:02:54.28 CTV.dial :air @15:02:54.58 PSV.dial :air @15:02:54.76 CSV.dial :waste1 @15:02:55.56 SSV.dial :spit,avoiding: :exhaust @15:02:55.65 PS.reconfigure PSconfig @15:02:55.74
Forearm.reconfigure LooseForearm @15:02:56.25 Forearm.home.seek :retract @15:02:56.34 PS.home.seek 0ml @15:02:58.36 Hand.close @15:02:58.67 CS.reconfigure CSfast @15:02:58.79 SS.reconfigure SSconfig @15:02:58.99
Elbow.jog 1500 @15:02:59.51 CS.home.seek 0ml @15:02:59.75 SS.home.seek 0ml @15:03:00.32
Elbow.reconfigure LooseElbow @15:03:00.52 Elbow.home.seek :clear @15:03:01.20 Session Begun @15:03:05.51
SE.reconfigure SEconfig @15:03:05.87 SE.home.seek :down @15:03:05.93 PS.coast @15:03:06.05 -> showlog 100 @15:03:07.09
SC.reconfigure SCsloppy @15:03:07.36 SC.home.seek :initial @15:03:17.89 Forearm.seek :retract @15:03:18.24 Elbow.seek :hammer @15:03:19.72 Forearm.reconfigure TightForearm @15:03:19.93 Forearm.seek 5 + 350 counts @15:03:21.27 Forearm.seek :retract @15:03:23.15 PC.reconfigure PCconfig @15:03:23.22
CC.reconfigure CCconfig @15:03:23.49 PC.home.seek :open @15:03:23.83
CC.home.seek :open @15:03:25.84 -> exit @15:03:25.90 Session Ended @15:03:28.12
Phase [coreNEG] @15:03:28.19 GoodNews.email "At 15:03:27 began in tube 2: coreNEG\n/home/esp/esp2/mission/InvasivePostCoreNeg.rb:113\n{}\nCan@15:03:28, 22.4C, 44% humidity, 14.7psia, 12.031V, 0.537A, 0.544A avg, 6.47W",Subject: "Started InvasivePostCoreNeg phase coreNEG" @15:03:28.25CEST13-Jul-20
==================> STARTED coreNEG (phase #1) <=== @15:03:28.64 Starting mfb @15:03:28.67 pcrBAC wcr sampling at most 200ml @15:03:29.07 Loading sh1 puck from tube 2 into Collection Clamp @15:03:29.34 Readying the MFB @15:03:29.40
Hand.open @15:03:29.46 Homing MFB Syringe @15:03:29.62 AFV.select :holdingCoil @15:03:30.10 ATV.dial :air2 @15:03:31.24 AS.reconfigure ASconfig @15:03:31.55 AS.home.seek :home @15:03:32.22
SC.reconfigure SCloose @15:03:33.14 Elbow.reconfigure TightElbow @15:03:33.31 Elbow.seek 2 @15:03:34.52 Forearm.seek 2 + 700 counts @15:03:35.90 SE.reconfigure SEpick @15:03:36.18 SE.seek beamBreak @15:03:36.98 BACfans.email "Started mfb in tube 2 at 15:03:28CEST13-Jul-20\n{}\nCan@15:03:28, 22.4C, 44% humidity, 14.7psia, 12.031V, 0.537A, 0.544A avg, 6.47W",Subject: "pcrBAC wcr sampling at most 200ml" @15:03:37.24 AFV.select :carrierSolution @15:03:37.48 delay 2 seconds @15:03:39.72
SE.configure SEconfig @15:03:40.49 AS.seek 1ml @15:03:41.51
Elbow.seek 2 @15:03:43.00 Forearm.seek 2 + 300 counts @15:03:43.39 Forearm.reconfigure LooseForearm @15:03:43.86 Hand.close @15:03:44.85 SE.seek :down @15:03:46.06
Forearm.seek :retract @15:03:47.31 Elbow.seek CC @15:03:48.24 SE.coast @15:03:49.35
Elbow.reconfigure LooseElbow @15:03:49.79 Forearm.reconfigure TightForearm @15:03:50.21 Forearm.seek CC @15:03:53.64 CC.seek :closed @15:04:03.68 Session Begun @15:05:38.28 delay 1 second @15:05:38.56 Clamp::VelocityError in MAIN -- Collection Clamp always accelerating!?! @15:05:38.61
Retry #1 of 2 @15:05:39.08 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[6E:27->20] @15:05:39.17 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[6E:27->20] @15:05:39.70 AFV.select :holdingCoil @15:05:40.05
CC.seek :open @15:05:40.29 delay 1 second @15:05:41.33 ATV.dial :waste @15:05:42.62 AS.reconfigure ASnorm @15:05:42.83 AS.home.seek :empty @15:05:44.70
CC.seek :closed @15:05:49.63 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.43 @15:05:49.69 Hand.open @15:05:50.84 Forearm.reconfigure LooseForearm @15:05:51.07 Forearm.seek :retract @15:05:52.29 SC.coast @15:05:52.47 skipping prime and sample collection @15:05:52.53 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @15:05:52.63 Intake.select :close @15:05:54.67 CSV.dial :waste @15:05:54.88 CS.seek 0ml @15:05:55.42 CSV.dial :air @15:05:55.64
Calibrating pressure and temperature sensors @15:05:55.84 CTV.dial :air @15:05:55.88 SSV.dial :spit,avoiding: :exhaust @15:05:56.09 SS.reconfigure SSfast @15:05:56.31 SS.seek 5ml @15:05:56.80 CS.seek 10ml @15:05:59.47 SSV.dial CBV @15:05:59.56
CBV.dial SSV @15:06:01.41 delay 3.8 seconds @15:06:05.61 SSV.dial :spit @15:06:07.25 SS.seek 0ml @15:06:10.32 SSV.dial CBV @15:06:12.03 SSV.dial CBV,avoiding: :exhaust @15:06:12.12
CBV.dial SSV @15:06:12.19 CTV.dial :air @15:06:12.32 delay 15 seconds @15:06:25.53 -> ls @15:06:25.59 NameError in mwj -- undefined local variable or method `ls' for # @15:06:27.40
SSV.dial :spit,avoiding: :exhaust @15:06:29.10 CTV.dial CSV,avoiding: :intake @15:06:29.25
SS.reconfigure SSexhale @15:06:29.61 SS.seek 0ml @15:06:29.91 SSV.dial CBV,avoiding: :exhaust @15:06:31.79 CBV.dial between SSV and waste2 @15:06:32.60 CTV.dial CSV,avoiding: :air @15:06:32.69
CSV.dial CTV @15:06:33.44 CS.reconfigure CSpressurize @15:06:33.67 CS.seek 0ml @15:06:35.65 ATV.dial :waste @15:06:35.71 ARV.dial between carrier and air1 @15:06:35.99 ASV.dial :waste1 @15:07:00.13
CSV.dial :air @15:07:00.69 CS.reconfigure CSfast @15:07:00.76 CS.seek 10ml @15:07:27.64 CSV.dial between air and CTV @15:07:28.02 CS.seek 3.353ml @15:07:46.24 CTV.dial CSV,avoiding: :air @15:07:46.27
CSV.dial CTV @15:07:46.64 CS.reconfigure CSpressurize @15:07:46.71 CS.seek 0ml @15:07:56.16 CSV.dial :air @15:07:56.71 CS.reconfigure CSfast @15:07:56.79 CS.seek 10ml @15:08:23.69 CSV.dial between air and CTV @15:08:24.08 CS.seek 2.088ml @15:08:45.43 CTV.dial CSV,avoiding: :air @15:08:45.46
CSV.dial CTV @15:08:45.83 CS.reconfigure CSpressurize @15:08:45.90 CS.seek 0ml @15:08:47.07 BubblePoint: Intake @70.2psia, Outlet @14.9psia @15:08:47.08 CBV.dial SSV @15:08:47.49 SS.reconfigure SSconfig @15:08:47.56 SS.seek 10ml @15:08:53.81 delay 15 seconds @15:09:08.96 Evacuated: Intake @28.9psia, Outlet @29.3psia @15:09:08.97 SSV.dial :spit,avoiding: :exhaust @15:09:10.52 SS.reconfigure SSexhale @15:09:10.59 SS.seek 0ml @15:09:15.82 SS.reconfigure SSfast @15:09:15.88 SS.seek 5ml @15:09:18.55 SSV.dial CBV,avoiding: :exhaust @15:09:18.59
CBV.dial SSV @15:09:20.13 CTV.dial :air,avoiding: :intake @15:09:21.71 CSV.dial CTV @15:09:21.75
SS.seek 10ml @15:09:22.75 CSV.dial :air @15:09:23.30 CS.reconfigure CSfast @15:09:23.83 CS.seek 0ml @15:09:24.51
SSV.dial :spit,avoiding: :exhaust @15:09:26.05 SS.reconfigure SSexhale @15:09:26.13 SS.seek 0ml @15:09:29.73 CS.coast @15:09:31.26
SS.reconfigure SSfast @15:09:31.33 SS.seek 5ml @15:09:34.17 SSV.dial CBV,avoiding: :exhaust @15:09:34.20
CBV.dial SSV @15:09:36.75 SSV.dial :spit,avoiding: :exhaust @15:09:38.28 SS.reconfigure SSconfig @15:09:38.35 SS.seek 0ml @15:09:41.54 SS.coast @15:09:41.60 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @15:09:41.61 Intake.select :close @15:09:41.73 CSV.dial :waste @15:09:41.88 SSV.dial CBV,avoiding: :exhaust @15:09:41.92
CBV.dial SSV @15:09:42.66 CS.seek 0ml @15:09:42.76 CSV.dial :air @15:09:43.46
CTV.dial :air @15:09:43.48 delay 15 seconds @15:09:43.63 CS.seek 10ml @15:09:58.51
SSV.dial :spit,avoiding: :exhaust @15:10:00.05 CTV.dial CSV,avoiding: :intake @15:10:00.13
SS.reconfigure SSexhale @15:10:00.23 SS.seek 0ml @15:10:00.31 SSV.dial CBV,avoiding: :exhaust @15:10:10.83 CBV.dial between SSV and waste2 @15:10:11.30 CTV.dial CSV,avoiding: :air @15:10:11.34
CSV.dial CTV @15:10:12.00 CS.reconfigure CSpressurize @15:10:12.07 CS.seek 0ml @15:10:38.29 CSV.dial :air @15:10:38.83 CS.reconfigure CSfast @15:10:38.91 CS.seek 10ml @15:11:05.49 CSV.dial between air and CTV @15:11:05.86 CS.seek 3.353ml @15:11:23.99 CTV.dial CSV,avoiding: :air @15:11:24.02
CSV.dial CTV @15:11:24.40 CS.reconfigure CSpressurize @15:11:24.47 CS.seek 0ml @15:11:33.92 CSV.dial :air @15:11:34.47 CS.reconfigure CSfast @15:11:34.55 CS.seek 10ml @15:12:01.11 CSV.dial between air and CTV @15:12:01.49 CS.seek 2.094ml @15:12:23.08 CTV.dial CSV,avoiding: :air @15:12:23.11
CSV.dial CTV @15:12:23.49 CS.reconfigure CSpressurize @15:12:23.56 CS.seek 0ml @15:12:24.94 BubblePoint: Intake @70.9psia, Outlet @15.4psia @15:12:24.95 CBV.dial SSV @15:12:25.35 SS.reconfigure SSconfig @15:12:25.42 SS.seek 10ml @15:12:31.54 delay 15 seconds @15:12:46.68 Evacuated: Intake @29.2psia, Outlet @29.6psia @15:12:46.69 SSV.dial :spit,avoiding: :exhaust @15:12:48.25 SS.reconfigure SSexhale @15:12:48.32 SS.seek 0ml @15:12:53.43 SS.reconfigure SSfast @15:12:53.50 SS.seek 5ml @15:12:56.15 SSV.dial CBV,avoiding: :exhaust @15:12:56.19
CBV.dial SSV @15:12:57.74 CTV.dial :air,avoiding: :intake @15:12:59.30 CSV.dial CTV @15:12:59.35
SS.seek 10ml @15:13:00.34 CSV.dial :air @15:13:00.88 CS.reconfigure CSfast @15:13:00.95 CS.seek 0ml @15:13:02.35
SSV.dial :spit,avoiding: :exhaust @15:13:03.89 SS.reconfigure SSexhale @15:13:03.97 SS.seek 0ml @15:13:07.07 CS.coast @15:13:09.09
SS.reconfigure SSfast @15:13:09.16 SS.seek 5ml @15:13:12.21 SSV.dial CBV,avoiding: :exhaust @15:13:12.26
CBV.dial SSV @15:13:14.75 SSV.dial :spit,avoiding: :exhaust @15:13:16.30 SS.reconfigure SSconfig @15:13:16.37 SS.seek 0ml @15:13:19.44 SS.coast @15:13:19.50 Lysing: Adding 1.5ml lysis @15:13:19.52 CBV.dial between CSV and waste1,avoiding: :DBV @15:13:21.29 CTV.dial CSV @15:13:22.84 CSV.dial CSR @15:13:23.76 Pulling 1ml of CSR.lysis @15:13:23.77 CSR.select :lysis @15:13:23.93 CS.reconfigure CSconfig @15:13:24.00 CS.seek 1ml @15:13:27.54 delay 5 seconds @15:13:32.57 CSR.select :air @15:13:32.77 CS.seek 6ml @15:13:46.48 delay 2 seconds @15:13:48.50 CSV.dial :waste1 @15:13:50.02 CS.reconfigure CSfast @15:13:50.09 CS.seek :empty @15:14:06.23 CSV.dial CSR @15:14:07.82 Pulling 1.5ml of CSR.lysis @15:14:07.83 CSR.select :lysis @15:14:07.98 CS.reconfigure CSconfig @15:14:08.05 CS.seek 1.5ml @15:14:13.01 delay 5 seconds @15:14:18.04 CSR.select :air @15:14:18.24 CS.seek 4.5ml @15:14:26.89 delay 2 seconds @15:14:28.91 CSV.dial CTV @15:14:29.44 CS.seek 1.275ml @15:14:38.74 Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @15:14:38.77 CH.seek 85C,8:00 @15:17:55.12 CH.hold 5:00,85C @15:22:55.32 Collection Puck Heater at 85.1C after lysing @15:22:55.34 CS.seek 0ml @15:22:59.76 CTV.dial between spare and air,avoiding: :intake @15:23:00.97 CSV.dial :air @15:23:01.51 CS.seek 5ml @15:23:15.52 CSV.dial CTV @15:23:16.06 delay 2 seconds @15:23:18.08 CSV.dial :air @15:23:18.63 delay 2 seconds @15:23:20.65 CSV.dial CTV @15:23:21.31 delay 2 seconds @15:23:23.33 CSV.dial :air @15:23:23.86 delay 2 seconds @15:23:25.89 CS.seek 0ml @15:23:39.67 Recovering Lysate into Collection syringe @15:23:39.68 CSV.dial CBV @15:23:40.89 CBV.dial CSV @15:23:41.35 CS.seek 5ml @15:23:55.24 delay 5 seconds @15:24:00.27 CTV.dial :air @15:24:00.88 CS.seek 7ml @15:24:06.99 delay 5 seconds @15:24:12.02 CSV.dial :air2 @15:24:12.56 CS.seek 10ml @15:24:21.27 CSV.dial between air and CTV,avoiding: CBV @15:24:22.65 Lysing Complete @15:24:22.74 Storing sh1 puck in Collection Clamp to tube 1 @15:24:22.95 SC.reconfigure SCtight @15:24:23.02 SC.seek 1 @15:24:25.17 SC.reconfigure SCloose @15:24:25.42 Elbow.reconfigure TightElbow @15:24:25.49 Elbow.seek 1 @15:24:27.33 Forearm.reconfigure TightForearm @15:24:27.40 Forearm.seek 1 + 700 counts @15:24:28.55 SE.reconfigure SEpick @15:24:28.71 SE.seek beamBreak @15:24:31.69 SE.configure SEconfig @15:24:31.77 SE.seek :place @15:24:32.34 Forearm.reconfigure LooseForearm @15:24:32.40 Forearm.seek :retract @15:24:33.37 Elbow.seek CC @15:24:35.04 Elbow.reconfigure LooseElbow @15:24:35.17 Forearm.seek CC @15:24:36.27 Hand.close @15:24:36.99 CC.seek :open @15:24:41.52 Forearm.seek :retract @15:24:42.59 Elbow.reconfigure TightElbow @15:24:42.66 Elbow.seek 1 @15:24:44.60 Elbow.reconfigure LimpElbow @15:24:44.68 Forearm.seek 1 @15:24:45.72 Elbow.hold @15:24:45.83 Elbow.reconfigure TightElbow @15:24:45.89 Hand.open @15:24:46.75 Forearm.force -20 @15:24:47.24 Forearm.seek 1 + 349 counts @15:24:47.68 Forearm.reconfigure TightForearm @15:24:47.75 Forearm.seek 1 + 700 counts @15:24:48.06 SE.seek :down @15:24:49.21
Forearm.reconfigure LooseForearm @15:24:49.28 Forearm.seek :retract @15:24:51.00 SE.coast @15:24:51.05
SC.coast @15:24:51.11 Tranferring lysate to PRV @15:24:51.12 CSV.dial :air @15:24:51.48 CS.seek 10ml @15:24:51.58 CSV.dial between air and CTV @15:24:51.97 AIV.dial :bypass @15:24:52.09 Beginning samplePassC @15:24:52.10 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:24:52.21 Hand.open @15:24:52.62 Elbow.seek FlushPuck::Garage @15:24:54.66 Elbow.reconfigure LooseElbow @15:24:54.78 Forearm.seek :garage @15:24:55.86 Hand.close @15:24:56.75 Forearm.seek :retract @15:24:57.85 Elbow.reconfigure TightElbow @15:24:57.92 Elbow.seek CC @15:25:00.87 Elbow.reconfigure LooseElbow @15:25:00.99 Forearm.reconfigure TightForearm @15:25:01.06 Forearm.seek CC @15:25:02.63 CC.seek :closed @15:25:06.98 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.39 @15:25:07.07 Hand.open @15:25:07.94 Forearm.reconfigure LooseForearm @15:25:08.01 Forearm.seek :retract @15:25:09.03 AWV.select :air @15:25:09.04 AIV.dial :bypass @15:25:09.06 PSV.dial PSR @15:25:10.66 Pulling 1.5ml of PSR.flush @15:25:10.67 PSR.select :flush @15:25:10.83 PS.seek 1.5ml @15:25:27.22 delay 5 seconds @15:25:32.25 PSV.dial :waste1 @15:25:32.79 PS.seek 0ml @15:25:49.16 PSV.dial PSR @15:25:49.69 PSR.select :air @15:25:49.83 PS.seek 2.5ml @15:26:16.48 PRV.dial CBV @15:26:16.51
CBV.dial PRV @15:26:17.46 PSV.dial :mixloop @15:26:18.64 CTV.dial :air,avoiding: :intake @15:26:18.65 PTV.dial :PRVmixing @15:26:18.78 PS.seek 0ml @15:26:45.38 PSV.dial :air @15:26:45.90 PS.seek 2.5ml @15:27:12.50 PSV.dial :mixloop @15:27:13.03 PS.seek 0ml @15:27:39.72 PSV.dial :air @15:27:40.25 PS.seek 2.5ml @15:28:06.88 PSV.dial :mixloop @15:28:07.40 PS.seek 0ml @15:28:34.10 Garaging FlushPuck in Collection Clamp @15:28:34.73 Elbow.reconfigure TightElbow @15:28:34.80 Elbow.seek CC @15:28:35.06 Elbow.reconfigure LooseElbow @15:28:35.19 Forearm.seek CC @15:28:36.25 Hand.close @15:28:37.00 CC.seek :open @15:28:41.43 Forearm.seek :retract @15:28:42.49 Elbow.reconfigure TightElbow @15:28:42.56 Elbow.seek :garage @15:28:45.61 Elbow.reconfigure LooseElbow @15:28:45.74 Forearm.reconfigure TightForearm @15:28:45.80 Forearm.seek :garage @15:28:46.90 Hand.open @15:28:47.68 Forearm.reconfigure LooseForearm @15:28:47.75 Forearm.seek :retract @15:28:48.79 Connecting PS to CS to transfer sample @15:28:48.80 CBV.dial AIV @15:28:48.83 AIV.dial :bypass @15:28:48.86 PSV.dial :mixloop @15:28:48.88 PTV.dial :PRVmixing @15:28:48.91 PRV.dial AIV @15:28:48.93 CTV.dial :air,avoiding: :intake @15:28:48.97 Loading lyfil puck from tube 2 into Collection Clamp @15:28:49.26 SC.reconfigure SCtight @15:28:49.34 SC.seek 2 @15:28:51.46 SC.reconfigure SCloose @15:28:51.70 Elbow.reconfigure TightElbow @15:28:51.77 Elbow.seek 2 @15:28:53.87 Forearm.reconfigure TightForearm @15:28:53.95 Forearm.seek 2 + 700 counts @15:28:54.97 SE.reconfigure SEpick @15:28:55.07 SE.seek beamBreak @15:28:58.24 SE.configure SEconfig @15:28:58.42 Elbow.seek 2 @15:28:58.64 Forearm.seek 2 + 300 counts @15:28:58.92 Forearm.reconfigure LooseForearm @15:28:58.98 Hand.close @15:28:59.76 SE.seek :down @15:29:00.00
Forearm.seek :retract @15:29:00.97 Elbow.seek CC @15:29:02.62 SE.coast @15:29:02.76
Elbow.reconfigure LooseElbow @15:29:02.88 Forearm.reconfigure TightForearm @15:29:02.95 Forearm.seek CC @15:29:04.30 CC.seek :closed @15:29:08.64 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.44 @15:29:08.65 Hand.open @15:29:09.56 Forearm.reconfigure LooseForearm @15:29:09.63 Forearm.seek :retract @15:29:10.69 SC.coast @15:29:10.75 CTV.dial CSV,avoiding: :intake @15:29:12.30 CSV.dial CTV @15:29:12.67 CS.seek 5ml @15:29:26.58 delay 1:20 @15:30:46.62 CS.seek 2.5ml @15:30:54.65 delay 40 seconds @15:31:34.69 CS.seek 0ml @15:31:42.40 delay 1:00 @15:32:42.43 PSV.dial :air @15:32:42.97 PS.seek 2.5ml @15:33:09.66 PSV.dial :mixloop @15:33:10.19 delay 3 seconds @15:33:13.22 PSV.dial :air @15:33:13.74 delay 2 seconds @15:33:15.76 PSV.dial :mixloop @15:33:16.28 delay 3 seconds @15:33:19.31 PSV.dial :waste1 @15:33:20.84 PS.seek :empty @15:33:47.50 CBV.dial :waste1 @15:33:48.05 delay 5 seconds @15:33:53.08 CBV.dial AIV @15:33:53.65 delay 10 seconds @15:34:03.68 CBV.dial :waste1 @15:34:04.22 delay 10 seconds @15:34:14.25 CBV.dial AIV @15:34:14.80 delay 10 seconds @15:34:24.83 CTV.dial CSV,avoiding: :intake @15:34:24.85 CBV.dial between AIV and waste1 @15:34:25.26 CSV.dial :air @15:34:25.81 CS.seek 10ml @15:34:52.65 CSV.dial CTV @15:34:53.20 CS.seek 0ml @15:35:20.27 CBV.dial :waste1 @15:35:20.63 delay 10 seconds @15:35:30.66 CTV.dial :air,avoiding: :intake @15:35:32.30 Storing lyfil puck in Collection Clamp to tube 1 @15:35:32.50 SC.reconfigure SCtight @15:35:32.57 SC.seek 1 @15:35:34.71 SC.reconfigure SCloose @15:35:34.95 Elbow.reconfigure TightElbow @15:35:35.02 Elbow.seek 1 @15:35:36.94 Forearm.reconfigure TightForearm @15:35:37.02 Forearm.seek 1 + 700 counts @15:35:38.05 SE.reconfigure SEpick @15:35:38.15 SE.seek beamBreak @15:35:41.33 SE.configure SEconfig @15:35:41.52 Elbow.reconfigure LimpElbow @15:35:41.58 Forearm.reconfigure LooseForearm @15:35:41.65 Forearm.seek 1 @15:35:41.96 Elbow.hold @15:35:42.06 Elbow.reconfigure TightElbow @15:35:42.18 SE.seek 0.07 Pucks below Top Plate @15:35:42.67 Forearm.force -20 @15:35:42.74 Forearm.seek 1 + 350 counts @15:35:43.09 Forearm.reconfigure TightForearm @15:35:43.16 Forearm.seek 1 + 700 counts @15:35:43.40 SE.seek 1.07 Pucks below Top Plate @15:35:44.15 Forearm.reconfigure LooseForearm @15:35:44.22 Forearm.seek :retract @15:35:45.19 Elbow.seek CC @15:35:46.90 Elbow.reconfigure LooseElbow @15:35:47.03 Forearm.seek CC @15:35:48.12 Hand.close @15:35:48.88 CC.seek :open @15:35:53.43 Forearm.seek :retract @15:35:55.00 Elbow.reconfigure TightElbow @15:35:55.07 Elbow.seek 1 @15:35:57.01 Elbow.reconfigure LimpElbow @15:35:57.08 Forearm.seek 1 @15:35:58.17 Elbow.hold @15:35:58.28 Elbow.reconfigure TightElbow @15:35:58.35 Hand.open @15:35:59.25 Forearm.force -20 @15:35:59.31 Forearm.seek 1 + 338 counts @15:35:59.67 Forearm.reconfigure TightForearm @15:35:59.74 Forearm.seek 1 + 700 counts @15:36:00.06 SE.seek :down @15:36:01.33
Forearm.reconfigure LooseForearm @15:36:01.47 Forearm.seek :retract @15:36:02.83 SE.coast @15:36:02.88
SC.coast @15:36:02.93 PSV.dial :air @15:36:04.14 PS.seek 0.499ml @15:36:10.13 delay 3 seconds @15:36:13.15 PSV.dial :mixloop @15:36:13.64 PRV.dial :air @15:36:14.21 delay 3 seconds @15:36:17.25 PRV.dial CBV @15:36:17.93 CBV.dial AIV @15:36:18.65 PRV.dial between CBV and air @15:36:19.20 PRV.dial :air @15:36:19.71 PS.reconfigure PSslow @15:36:19.78 PS.seek 0.599ml @15:36:22.41 PRV.dial :waste2 @15:36:23.31 PS.reconfigure PSconfig @15:36:23.37 PS.seek :empty @15:36:30.32 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:36:30.42 Hand.open @15:36:30.75 Elbow.seek FlushPuck::Garage @15:36:32.79 Elbow.reconfigure LooseElbow @15:36:32.91 Forearm.seek :garage @15:36:34.00 Hand.close @15:36:34.88 Forearm.seek :retract @15:36:35.95 Elbow.reconfigure TightElbow @15:36:36.02 Elbow.seek CC @15:36:38.99 Elbow.reconfigure LooseElbow @15:36:39.11 Forearm.reconfigure TightForearm @15:36:39.18 Forearm.seek CC @15:36:40.57 CC.seek :closed @15:36:44.89 Collection Clamp onSpring/offSpring for 5/31 samples, on/off torque 2.34 @15:36:44.99 Hand.open @15:36:46.30 Forearm.reconfigure LooseForearm @15:36:46.41 Forearm.seek :retract @15:36:47.54 Clearing PML for modified lysate @15:36:47.55 CTV.dial :air,avoiding: :intake @15:36:47.57 PRV.dial :waste2 @15:36:47.61 PTV.dial :PRV,avoiding: :puck @15:36:48.49 delay 2 seconds @15:36:50.51 PTV.dial :PRVmixing,avoiding: :puck @15:36:51.37 delay 2 seconds @15:36:53.39 PSV.dial :PML @15:36:53.41 delay 2 seconds @15:36:55.45 PRV.dial :air @15:36:56.30 delay 2 seconds @15:36:58.38 PS.seek 1.499ml @15:37:14.66 delay 5 seconds @15:37:19.70 PS.seek 2.5ml @15:37:30.79 PSV.dial :air @15:37:31.33 PS.seek 0ml @15:37:58.04 mixing lysate with speDil @15:37:58.05 PSV.dial :PML @15:37:58.60 CBV.dial :PRV @15:37:58.62 PRV.dial :air @15:37:58.65 delay 2 seconds @15:38:00.68 PRV.dial :speDil @15:38:01.22 delay 2 seconds @15:38:03.32 PS.reconfigure PSslow1 @15:38:03.40 PS.seek 0.225ml @15:38:27.75 delay 5 seconds @15:38:32.78 PRV.dial CBV @15:38:33.66 delay 2 seconds @15:38:35.75 PS.seek 0.475ml @15:39:01.97 delay 5 seconds @15:39:07.00 PRV.dial :air @15:39:07.54 delay 2 seconds @15:39:09.57 PS.seek 0.965ml @15:39:59.75 delay 10 seconds @15:40:09.78 PRV.dial CBV @15:40:10.30 CTV.dial :air,avoiding: :intake @15:40:10.32 PSV.dial :air @15:40:10.87 PS.reconfigure PSconfig @15:40:10.94 PS.seek 2.5ml @15:40:27.29 delay 3 seconds @15:40:30.31 PSV.dial PTV @15:40:31.16 PTV.dial :PRV,avoiding: :puck @15:40:32.02 PS.seek 0ml @15:40:58.38 PSV.dial :air @15:40:59.25 PS.seek 2.5ml @15:41:25.66 delay 3 seconds @15:41:28.68 PSV.dial PTV @15:41:29.54 PTV.dial :PRV,avoiding: :puck @15:41:29.57 PS.seek 0ml @15:41:56.01 Pushing modified lysate into AIV Injection Loop @15:41:56.02 CBV.dial AIV @15:41:56.06 AIV.dial :bypass @15:41:56.08 PRV.dial CBV @15:41:56.11 PSV.dial :air @15:41:56.98 PS.seek 2.5ml @15:42:23.38 delay 3 seconds @15:42:26.40 PSV.dial :PML @15:42:26.93 PTV.dial :PRVmixing,avoiding: :puck @15:42:27.86 PS.reconfigure PSslow1 @15:42:27.93 PS.seek 1.74ml @15:43:45.26 delay 5 seconds @15:43:50.29 AIV.dial :sample @15:43:50.97 AWV.select :waste @15:43:51.17 PS.reconfigure PSslow2 @15:43:51.24 PS.seek 1.22ml @15:45:39.07 delay 15 seconds @15:45:54.10 AIV.dial :bypass @15:45:54.77 PS.reconfigure PSconfig @15:45:54.84 PS.seek 0ml @15:46:08.07 PSV.dial :air @15:46:08.61 PS.seek 2.5ml @15:46:35.01 PSV.dial :PML @15:46:35.55 PS.seek 0ml @15:47:01.73 AIV.dial :coilToMFB @15:47:02.20 AWV.select :air @15:47:02.44 Extracting Nucleic Acid from AIV @15:47:02.47
Beginning samplePassD @15:47:02.51 SPE.hold 2:46:40,55C @15:47:02.63 ASV.dial :SPEdelivery @15:47:02.73
AWV.select :air @15:47:02.74 AIV.dial :bypass @15:47:02.77 PSV.dial PSR @15:47:03.10 delay 20 seconds @15:47:04.11
Pulling 1.5ml of PSR.killMFB @15:47:04.12 PSR.select :killMFB @15:47:04.29 PS.seek 1.5ml @15:47:21.42 delay 5 seconds @15:47:23.13 primeLysateINT @15:47:23.15 AWV.select :air @15:47:23.17 AIV.dial :coilToMFB @15:47:23.20 ATV.dial AIV @15:47:25.23 AS.reconfigure ASslow1 @15:47:25.31 AS.seek 0.299ml @15:47:26.44
PSV.dial :waste1 @15:47:27.00 PS.seek 0ml @15:47:44.10 PSV.dial PSR @15:47:44.66 PSR.select :air @15:47:44.81 PS.seek 2.5ml @15:48:12.88 PRV.dial CBV @15:48:12.91
CBV.dial PRV @15:48:12.95 PSV.dial :mixloop @15:48:14.19 CTV.dial :air,avoiding: :intake @15:48:14.21 PTV.dial :PRVmixing @15:48:14.25 PS.seek 0ml @15:48:25.97 delay 10 seconds @15:48:36.00 ATV.dial :air2 @15:48:36.95 AS.seek 0.329ml @15:48:42.10
PSV.dial PSR @15:48:43.30 ATV.dial :waste2 @15:48:43.46
Pulling 1.5ml of PSR.flush @15:48:43.47 PSR.select :flush @15:48:43.63 PS.seek 1.5ml @15:48:43.95 AS.reconfigure ASnorm @15:48:44.01 AS.seek :empty @15:49:00.85
delay 5 seconds @15:49:01.08 Lysate primed! @15:49:01.10 AFV.select :holdingCoil @15:49:01.12 ATV.dial :air2 @15:49:01.82 AS.reconfigure ASslow1 @15:49:01.89 AS.seek 0.069ml @15:49:05.88
PSV.dial :waste1 @15:49:06.44 PS.seek 0ml @15:49:16.33 delay 3 seconds @15:49:19.36 ATV.dial :AIV @15:49:20.32 AS.seek 0.469ml @15:49:23.54
PSV.dial PSR @15:49:24.08 PSR.select :air @15:49:24.23 PS.seek 2.5ml @15:49:52.29 PRV.dial CBV @15:49:52.33
CBV.dial PRV @15:49:52.36 PSV.dial :mixloop @15:49:53.60 CTV.dial :air,avoiding: :intake @15:49:53.62 PTV.dial :PRVmixing @15:49:53.66 PS.seek 0ml @15:50:21.07 PSV.dial PSR @15:50:22.36 Pulling 1.5ml of PSR.flush @15:50:22.37 PSR.select :flush @15:50:22.54 PS.seek 1.5ml @15:50:39.35 delay 5 seconds @15:50:41.05 delay 5 seconds @15:50:44.38
PSV.dial :waste1 @15:50:44.94 PS.seek 0ml @15:50:46.12 Solid Phase Extraction Heater at 50.6C @15:50:46.13 ATV.dial ASV @15:50:46.58 ASV.dial :SPEdelivery @15:50:46.68 AS.seek 0.041ml @15:51:01.51
PSV.dial PSR @15:51:02.05 PSR.select :air @15:51:02.20 PS.seek 2.5ml @15:51:29.32 PRV.dial CBV @15:51:29.36
CBV.dial PRV @15:51:29.38 PSV.dial :mixloop @15:51:31.10 CTV.dial :air,avoiding: :intake @15:51:31.12 PTV.dial :PRVmixing @15:51:31.17 PS.seek 0ml @15:51:58.35 PSV.dial PSR @15:51:59.62 Pulling 1.5ml of PSR.flush @15:51:59.63 PSR.select :flush @15:51:59.79 PS.seek 1.5ml @15:52:13.05 delay 10 seconds @15:52:16.33
delay 5 seconds @15:52:21.35 PSV.dial :waste1 @15:52:21.89 PS.seek 0ml @15:52:23.07 rinseLysateLineINT @15:52:23.09 AFV.select :carrierSolution @15:52:23.20 delay 2 seconds @15:52:25.24 AS.reconfigure ASconfig @15:52:25.30 AS.seek 1ml @15:52:38.32
PSV.dial PSR @15:52:38.87 PSR.select :air @15:52:39.02 PS.seek 2.5ml @15:53:05.98 PRV.dial CBV @15:53:06.01
CBV.dial PRV @15:53:06.05 PSV.dial :mixloop @15:53:07.24 CTV.dial :air,avoiding: :intake @15:53:07.26 PTV.dial :PRVmixing @15:53:07.30 PS.reconfigure PSslow @15:53:07.38 PS.seek 1.2ml @15:53:20.69 delay 1 second @15:53:21.71 AFV.select :holdingCoil @15:53:21.82 delay 1 second @15:53:22.85 ATV.dial AIV @15:53:23.44 AIV.dial :coilToMFB @15:53:23.46 AWV.select :waste @15:53:23.60 AS.reconfigure ASslow0 @15:53:23.66 AS.seek 0ml @15:53:34.26
PSV.dial :waste1 @15:53:35.80 PS.reconfigure PSconfig @15:53:35.87 PS.seek 0ml @15:53:49.23 PSV.dial :air @15:53:50.44 PS.seek 2.5ml @15:54:17.35 PSV.dial :mixloop @15:54:17.88 PS.reconfigure PSslow @15:54:17.95 PS.seek 0ml @15:55:04.57 ATV.dial :air2 @15:55:05.29 AS.reconfigure ASslow1 @15:55:05.36 AS.seek 0.05ml @15:55:09.10
PSV.dial :air @15:55:09.65 PS.reconfigure PSconfig @15:55:09.72 PS.seek 2.5ml @15:55:15.69 ATV.dial :AIV @15:55:16.57 AS.seek 0.01ml @15:55:24.91 ATV.dial :waste @15:55:26.67 AS.seek :empty @15:55:29.13 ethanol wash @15:55:29.14 ATV.dial :air2 @15:55:30.48 AS.reconfigure ASslow2 @15:55:30.55 AS.seek 0.009ml @15:55:36.35
PSV.dial :mixloop @15:55:36.89 PS.reconfigure PSslow @15:55:36.96 PS.seek 0ml @15:55:41.00 delay 3 seconds @15:55:44.03 Pulling 0.4ml of Ethanol Wash @15:55:44.04 ATV.dial :ethanol @15:55:44.93 AS.reconfigure ASslow1 @15:55:45.00 AS.seek 0.409ml @15:56:28.10
Cleaning Collection Syringe @15:56:28.12 CSV.dial CSR @15:56:28.71 Pulling 4ml of CSR.flush @15:56:28.72 CSR.select :flush @15:56:28.88 CS.seek 4ml @15:56:40.47 delay 5 seconds @15:56:45.50 CSR.select :air @15:56:45.64 CS.seek 10ml @15:57:02.16 CSV.dial CTV @15:57:02.20
CTV.dial CSV @15:57:03.83 CBV.dial :waste1 @15:57:04.40 CS.seek 0ml @15:57:05.77 delay 3 seconds @15:57:08.79 ATV.dial :air1 @15:57:09.21 delay 3 seconds @15:57:12.30 AS.seek 0.434ml @15:57:17.63 delay 3 seconds @15:57:21.11 ATV.dial :ethanol @15:57:21.84 AS.seek 0.419ml @15:57:25.16 delay 3 seconds @15:57:28.19 ATV.dial ASV @15:57:29.60 AS.seek 0.092ml @15:57:31.13
CSV.dial CSR @15:57:31.74 Pulling 4ml of CSR.flush @15:57:31.75 CSR.select :flush @15:57:31.91 CS.seek 4ml @15:57:43.22 delay 5 seconds @15:57:48.25 CSR.select :air @15:57:48.40 CS.seek 10ml @15:58:05.00 CSV.dial CTV @15:58:05.04
CTV.dial CSV @15:58:05.58 CBV.dial :waste1 @15:58:05.61 CS.seek 0ml @15:58:32.29 Garaging FlushPuck in Collection Clamp @15:58:32.39 Elbow.reconfigure TightElbow @15:58:32.46 Elbow.seek CC @15:58:32.76 Elbow.reconfigure LooseElbow @15:58:32.89 Forearm.seek CC @15:58:33.96 Hand.close @15:58:34.78 CC.seek :open @15:58:35.64 delay 3 seconds @15:58:38.66 ATV.dial :waste2 @15:58:39.20
Forearm.seek :retract @15:58:39.47 AS.reconfigure ASconfig @15:58:39.53 AS.seek :empty @15:58:40.27
Elbow.reconfigure TightElbow @15:58:40.34 Elbow.seek :garage @15:58:43.39 Elbow.reconfigure LooseElbow @15:58:43.52 Forearm.reconfigure TightForearm @15:58:43.59 Forearm.seek :garage @15:58:44.77 Hand.open @15:58:45.22 ATV.dial :air2 @15:58:45.59
Forearm.reconfigure LooseForearm @15:58:45.69 Forearm.seek :retract @15:58:45.72 AS.reconfigure ASslow1 @15:58:45.83 AS.seek 1ml @15:58:46.83 Whole Cell Archive sampling 200ml @15:58:46.85 Loading wcr puck from tube 2 into Collection Clamp @15:58:47.15 SC.reconfigure SCtight @15:58:47.22 SC.seek 2 @15:58:49.44 SC.reconfigure SCloose @15:58:49.69 Elbow.reconfigure TightElbow @15:58:49.77 Elbow.seek 2 @15:58:51.90 Forearm.reconfigure TightForearm @15:58:51.98 Forearm.seek 2 + 700 counts @15:58:53.05 SE.reconfigure SEpick @15:58:53.16 SE.seek beamBreak @15:58:56.41 SE.configure SEconfig @15:58:56.66 Elbow.seek 2 @15:58:56.91 Forearm.seek 2 + 300 counts @15:58:57.21 Forearm.reconfigure LooseForearm @15:58:57.28 Hand.close @15:58:58.05 SE.seek :down @15:58:58.83 Forearm.seek :retract @15:58:59.84 Elbow.seek CC @15:59:01.52 Elbow.reconfigure LooseElbow @15:59:01.66 Forearm.reconfigure TightForearm @15:59:01.75 Forearm.seek CC @15:59:01.78 SE.coast @15:59:03.23 CC.seek :closed @15:59:07.50 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.50 @15:59:07.52 Hand.open @15:59:08.34 Forearm.reconfigure LooseForearm @15:59:08.42 Forearm.seek :retract @15:59:09.48 SC.coast @15:59:09.55 collectSample 200ml w/bubble point of 55psi @15:59:09.56 Priming 7ml @15:59:09.59 Intake.select :close @15:59:09.62 Exhaust.select :close @15:59:09.65 SS.hold @15:59:09.90 Calibrating pressure sensors @15:59:09.91 CTV.dial :air @15:59:11.47 SSV.dial :spit,avoiding: :exhaust @15:59:11.50 SS.reconfigure SSfast @15:59:11.60 SS.seek 5ml @15:59:14.35 SSV.dial CBV @15:59:14.39 CBV.dial SSV @15:59:16.05 delay 3.8 seconds @15:59:20.00 SSV.dial :spit @15:59:21.52 SS.seek 0ml @15:59:24.18 SSV.dial CBV @15:59:25.73 CTV.dial :intake @15:59:26.69 SSV.dial CBV @15:59:26.72 CBV.dial SSV @15:59:26.75 Allowing 40s for 7.0ml of remaining 7.0ml @15:59:26.78 Intake.select :open @15:59:28.98 Intake @15.5psia, vacuum from 9.0 to 13.0psi @15:59:29.01 SS.reconfigure SSinhale.with maxSpeed: 150 @15:59:29.09 SS.seek 7ml @15:59:35.10 Stroke completed -- waiting for pressure to equalize ... @15:59:53.37 Intake.select :close @15:59:53.55 SSV.dial :exhaust @15:59:54.44 Exhaust.select :open @15:59:54.58 SS.reconfigure SSexhale @15:59:54.65 SS.seek 0ml @15:59:59.10 Exhaust.select :close @15:59:59.24 Sampling 200.0ml @15:59:59.25 Intake.select :close @15:59:59.27 Exhaust.select :close @15:59:59.30 SS.reconfigure SSconfig @15:59:59.38 SS.hold @15:59:59.62 CTV.dial :intake @15:59:59.64 SSV.dial CBV @15:59:59.68 CBV.dial SSV @16:00:00.57 Allowing 130s for 25.0ml of remaining 200.0ml @16:00:00.59 Intake.select :open @16:00:02.81 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:00:02.83 SS.reconfigure SSinhale.with maxSpeed: 150 @16:00:02.93 SS.seek 25ml @16:00:07.11 Vacuum too high -- waiting up to 40s @16:00:16.84 Intake @16.1psia, vacuum from 9.6 to 13.6psi @16:00:16.87 SS.reconfigure SSinhale.with maxSpeed: 135 @16:00:16.95 SS.seek 25ml @16:00:20.36 Vacuum too high -- waiting up to 40s @16:00:29.93 Intake @16.1psia, vacuum from 9.6 to 13.6psi @16:00:29.95 SS.reconfigure SSinhale.with maxSpeed: 122 @16:00:30.04 SS.seek 25ml @16:00:33.64 Vacuum too high -- waiting up to 40s @16:00:44.70 SS.reconfigure SSinhale.with maxSpeed: 110 @16:00:44.78 SS.seek 25ml @16:00:48.51 Vacuum too high -- waiting up to 40s @16:00:59.66 Intake @16.1psia, vacuum from 9.6 to 13.6psi @16:00:59.68 SS.reconfigure SSinhale.with maxSpeed: 99 @16:00:59.77 SS.seek 25ml @16:01:03.96 Vacuum too high -- waiting up to 40s @16:01:15.79 Intake @16.0psia, vacuum from 9.5 to 13.5psi @16:01:15.81 SS.reconfigure SSinhale.with maxSpeed: 89 @16:01:15.90 SS.seek 25ml @16:01:19.17 Stroke completed -- waiting for pressure to equalize ... @16:01:30.66 Intake.select :close @16:01:30.83 SSV.dial :exhaust @16:01:31.73 Exhaust.select :open @16:01:31.87 SS.reconfigure SSexhale @16:01:31.94 SS.seek 0ml @16:01:41.68 Exhaust.select :close @16:01:41.82 SSV.dial CBV @16:01:41.85 CBV.dial SSV @16:01:42.76 Allowing 130s for 25.0ml of remaining 175.0ml @16:01:42.78 Intake.select :open @16:01:45.00 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:01:45.03 SS.reconfigure SSinhale.with maxSpeed: 89 @16:01:45.11 SS.seek 25ml @16:01:49.07 Vacuum too high -- waiting up to 40s @16:01:56.84 Intake @15.9psia, vacuum from 9.4 to 13.4psi @16:01:56.86 SS.reconfigure SSinhale.with maxSpeed: 80 @16:01:56.95 SS.seek 25ml @16:02:01.07 Vacuum too high -- waiting up to 40s @16:02:07.50 delay 5 seconds @16:02:08.59 Intake @15.9psia, vacuum from 9.4 to 13.4psi @16:02:08.61 SS.reconfigure SSinhale.with maxSpeed: 72 @16:02:08.70 SS.seek 25ml @16:02:12.52 ATV.dial ASV @16:02:13.07 Vacuum too high -- waiting up to 40s @16:02:13.61 ASV.dial :SPEdelivery @16:02:13.64 AS.seek 0.02ml @16:02:21.46 SS.reconfigure SSinhale.with maxSpeed: 65 @16:02:21.53 SS.seek 25ml @16:02:27.23 Vacuum too high -- waiting up to 40s @16:02:36.68 SS.reconfigure SSinhale.with maxSpeed: 59 @16:02:36.76 SS.seek 25ml @16:02:43.70 Vacuum too high -- waiting up to 40s @16:02:53.60 SS.reconfigure SSinhale.with maxSpeed: 53 @16:02:53.68 SS.seek 25ml @16:02:59.66 Stroke completed -- waiting for pressure to equalize ... @16:03:11.06 Intake.select :close @16:03:11.23 SSV.dial :exhaust @16:03:12.12 Exhaust.select :open @16:03:12.26 SS.reconfigure SSexhale @16:03:12.33 SS.seek 0ml @16:03:21.94 Exhaust.select :close @16:03:22.08 SSV.dial CBV @16:03:22.11 CBV.dial SSV @16:03:23.01 Allowing 130s for 25.0ml of remaining 150.0ml @16:03:23.03 Intake.select :open @16:03:25.25 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:03:25.27 SS.reconfigure SSinhale.with maxSpeed: 53 @16:03:25.36 SS.seek 25ml @16:03:33.11 Vacuum too high -- waiting up to 40s @16:03:40.16 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:03:40.18 SS.reconfigure SSinhale.with maxSpeed: 48 @16:03:40.26 SS.seek 25ml @16:03:48.20 Vacuum too high -- waiting up to 40s @16:03:56.70 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:03:56.72 SS.reconfigure SSinhale.with maxSpeed: 43 @16:03:56.81 SS.seek 25ml @16:04:09.39 Vacuum too high -- waiting up to 40s @16:04:18.42 Intake @15.8psia, vacuum from 9.3 to 13.3psi @16:04:18.44 SS.reconfigure SSinhale.with maxSpeed: 39 @16:04:18.53 SS.seek 25ml @16:04:32.30 Stroke completed -- waiting for pressure to equalize ... @16:04:44.91 Intake.select :close @16:04:45.08 SSV.dial :exhaust @16:04:45.97 Exhaust.select :open @16:04:46.11 SS.reconfigure SSexhale @16:04:46.19 SS.seek 0ml @16:04:55.85 Exhaust.select :close @16:04:55.99 SSV.dial CBV @16:04:56.02 CBV.dial SSV @16:04:56.93 Allowing 130s for 25.0ml of remaining 125.0ml @16:04:56.96 Intake.select :open @16:04:59.16 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:04:59.19 SS.reconfigure SSinhale.with maxSpeed: 39 @16:04:59.27 SS.seek 25ml @16:05:15.57 Vacuum too high -- waiting up to 40s @16:05:23.29 SS.reconfigure SSinhale.with maxSpeed: 35 @16:05:23.37 SS.seek 25ml @16:05:31.02 delay 5 seconds @16:05:36.05 ATV.dial :air2 @16:05:37.18 AS.seek 0.52ml @16:05:48.95 Vacuum too high -- waiting up to 40s @16:05:57.44 SS.reconfigure SSinhale.with maxSpeed: 32 @16:05:57.52 SS.seek 25ml @16:06:06.46 Stroke completed -- waiting for pressure to equalize ... @16:06:13.47 Intake.select :close @16:06:13.65 SSV.dial :exhaust @16:06:14.54 Exhaust.select :open @16:06:14.68 SS.reconfigure SSexhale @16:06:14.75 SS.seek 0ml @16:06:24.16 Exhaust.select :close @16:06:24.30 SSV.dial CBV @16:06:24.33 CBV.dial SSV @16:06:25.24 Allowing 130s for 25.0ml of remaining 100.0ml @16:06:25.27 Intake.select :open @16:06:27.47 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:06:27.49 SS.reconfigure SSinhale.with maxSpeed: 32 @16:06:27.59 SS.seek 25ml @16:07:18.05 delay 5 seconds @16:07:23.06 Stroke completed -- waiting for pressure to equalize ... @16:07:23.10 ATV.dial ASV @16:07:24.16 ASV.dial :wasteR @16:07:24.65 AS.seek 0.3ml @16:07:35.69 Intake.select :close @16:07:35.87 SSV.dial :exhaust @16:07:36.75 Exhaust.select :open @16:07:36.89 SS.reconfigure SSexhale @16:07:36.96 SS.seek 0ml @16:07:46.29 Exhaust.select :close @16:07:46.44 SSV.dial CBV @16:07:46.47 CBV.dial SSV @16:07:47.36 Allowing 130s for 25.0ml of remaining 75.0ml @16:07:47.38 Intake.select :open @16:07:49.60 Intake @15.7psia, vacuum from 9.2 to 13.2psi @16:07:49.62 SS.reconfigure SSinhale.with maxSpeed: 32 @16:07:49.71 SS.seek 25ml @16:08:09.22 delay 20 seconds @16:08:29.26 AS.seek 0.07ml @16:08:45.35 Stroke completed -- waiting for pressure to equalize ... @16:08:56.19 Intake.select :close @16:08:56.37 SSV.dial :exhaust @16:08:57.25 Exhaust.select :open @16:08:57.39 SS.reconfigure SSexhale @16:08:57.46 SS.seek 0ml @16:09:07.16 Exhaust.select :close @16:09:07.30 SSV.dial CBV @16:09:07.33 CBV.dial SSV @16:09:08.24 Allowing 130s for 25.0ml of remaining 50.0ml @16:09:08.26 Intake.select :open @16:09:10.47 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:09:10.50 SS.reconfigure SSinhale.with maxSpeed: 32 @16:09:10.58 SS.seek 25ml @16:09:15.80 delay 20 seconds @16:09:35.83 ATV.dial :waste2 @16:09:36.75 AS.reconfigure ASconfig @16:09:36.81 AS.seek :empty @16:09:41.22 Elution @16:09:41.23 ATV.dial :air1 @16:09:42.48 AS.reconfigure ASslow1 @16:09:42.55 AS.seek 0.049ml @16:09:52.96 delay 3 seconds @16:09:55.99 ATV.dial :ElutionWater @16:09:56.74 AS.seek 0.109ml @16:10:05.60 Stroke completed -- waiting for pressure to equalize ... @16:10:09.11 delay 3 seconds @16:10:12.45 ATV.dial ASV,via: :air2 @16:10:14.71 ASV.dial :SPEelution @16:10:15.12 Intake.select :close @16:10:15.30 SSV.dial :exhaust @16:10:15.39 AFV.select :carrierSolution @16:10:15.58 AS.reconfigure ASnorm @16:10:15.66 AS.seek 0.759ml @16:10:16.21 Exhaust.select :open @16:10:16.35 SS.reconfigure SSexhale @16:10:16.42 SS.seek 0ml @16:10:26.27 Exhaust.select :close @16:10:26.41 SSV.dial CBV @16:10:26.44 CBV.dial SSV @16:10:27.35 Allowing 130s for 25.0ml of remaining 25.0ml @16:10:27.37 Intake.select :open @16:10:29.59 Intake @15.6psia, vacuum from 9.1 to 13.1psi @16:10:29.62 SS.reconfigure SSinhale.with maxSpeed: 32 @16:10:29.70 SS.seek 24.99ml @16:10:48.72 delay 3 seconds @16:10:51.75 AFV.select :holdingCoil @16:10:51.93 AS.reconfigure ASslow1 @16:10:52.00 AS.seek 0.474ml @16:11:25.10 Stroke completed -- waiting for pressure to equalize ... @16:11:33.99 Intake.select :close @16:11:34.17 SSV.dial :exhaust @16:11:35.07 Exhaust.select :open @16:11:35.22 SS.reconfigure SSexhale @16:11:35.29 SS.seek 0ml @16:11:45.11 Exhaust.select :close @16:11:45.25 Sampled 200.0ml @16:11:45.27 Drying puck before fixing for 4:00 @16:11:45.29 CBV.dial :waste2b @16:11:46.20 delay 1:00 @16:11:49.63 delay 5 seconds @16:11:54.72 AS.reconfigure ASslow2 @16:11:54.80 AS.seek 0.439ml @16:12:30.17 delay 1:00 @16:12:46.23 CSV.dial :air @16:12:46.77 CS.reconfigure CSfast @16:12:46.84 CS.seek 10ml @16:13:13.61 Pumping wcr puck from tube 2 up to 55psi... @16:13:13.63 CBV.dial SSV @16:13:15.10 CBV.dial between CSV and waste2b @16:13:16.24 CSV.dial between air and CTV @16:13:16.63 CS.seek 7.231ml @16:13:24.83 CTV.dial CSV,avoiding: :air @16:13:24.86 CSV.dial CTV @16:13:25.79 CS.reconfigure CSpressurize @16:13:26.40 CS.seek 0ml @16:13:30.19 pushing eluate to holding coil @16:13:30.29 AS.seek 0.279ml @16:13:45.76 CSV.dial :air @16:13:46.32 CS.reconfigure CSfast @16:13:46.40 CS.seek 10ml @16:14:13.21 CSV.dial between air and CTV @16:14:13.61 CS.seek 2.271ml @16:14:34.52 CTV.dial CSV,avoiding: :air @16:14:34.56 CSV.dial CTV @16:14:34.93 CS.reconfigure CSpressurize @16:14:35.01 CS.seek 0ml @16:14:37.57 CBV.dial :waste2b @16:14:37.99 CS.reconfigure CSconfig @16:14:38.06 CS.seek :empty @16:14:43.01 delay 1:00 @16:15:43.04 CTV.dial :air,avoiding: :intake @16:15:44.62 Fix #1 of 2 (with rnal) for 2:00 @16:15:44.64 CSV.dial CSR @16:15:45.22 Pulling 2ml of CSR.rnal @16:15:45.23 CSR.select :rnal @16:15:45.40 CS.seek 2ml @16:15:51.54 delay 5 seconds @16:15:56.57 CSR.select :air @16:15:56.72 CS.reconfigure CSfast @16:15:56.79 CS.seek 10ml @16:16:11.38 delay 30 seconds @16:16:18.49 Pumping wcr puck from tube 2 up to 55psi... @16:16:18.54 CBV.dial SSV @16:16:20.35 CBV.dial between CSV and waste2b @16:16:21.50 CSV.dial between air and CTV @16:16:22.25 CS.seek 7.231ml @16:16:30.59 CTV.dial CSV,avoiding: :air @16:16:30.63 CSV.dial CTV @16:16:32.23 CS.reconfigure CSpressurize @16:16:32.31 CS.seek 0ml @16:16:41.43 ATV.dial :waste,via: :air3 @16:16:43.25 AS.reconfigure ASconfig @16:16:43.31 AS.seek :empty @16:16:51.64 CSV.dial :air @16:16:52.20 CS.reconfigure CSfast @16:16:52.28 CS.seek 10ml @16:16:59.74 Parking Bleach in LysateSource line and SPEcolumn @16:16:59.76 ATV.dial :air3 @16:17:00.65 AS.reconfigure ASslow1 @16:17:00.72 AS.seek 0.029ml @16:17:07.11 ATV.dial :ElutionWater @16:17:07.76 AS.seek 0.059ml @16:17:14.10 ATV.dial :air3 @16:17:14.74 AS.seek 0.069ml @16:17:17.05 delay 2 seconds @16:17:19.13 CSV.dial between air and CTV @16:17:19.20 ATV.dial :bleach @16:17:19.54 CS.seek 2.256ml @16:17:19.71 AS.seek 0.769ml @16:17:40.77 CTV.dial CSV,avoiding: :air @16:17:40.80 CSV.dial CTV @16:17:41.70 CS.reconfigure CSpressurize @16:17:41.78 CS.seek 0ml @16:17:44.20 delay 2:00 @16:19:40.75 delay 3 seconds @16:19:43.78 ATV.dial :air3 @16:19:44.22 CBV.dial :waste2b @16:19:44.36 delay 3 seconds @16:19:44.62 CS.reconfigure CSconfig @16:19:44.69 CS.seek :empty @16:19:47.45 AS.seek 0.794ml @16:19:49.77 delay 1:00 @16:19:52.78 delay 3 seconds @16:19:55.81 ATV.dial :bleach @16:19:56.46 AS.seek 0.779ml @16:19:59.78 delay 3 seconds @16:20:02.81 ATV.dial :AIV @16:20:04.15 AS.reconfigure ASconfig @16:20:04.22 AS.seek 0.015ml @16:20:48.42 ATV.dial :waste @16:20:49.80 CTV.dial :air,avoiding: :intake @16:20:50.01 AS.seek :empty @16:20:51.25 ATV.dial :air3 @16:20:51.37 Fix #2 of 2 (with rnal) for 2:00 @16:20:51.38 CSV.dial CSR @16:20:52.01 AS.reconfigure ASslow1 @16:20:52.13 AS.seek 0.009ml @16:20:52.16 Pulling 2ml of CSR.rnal @16:20:52.18 CSR.select :rnal @16:20:52.39 CS.seek 2ml @16:20:54.55 delay 2 seconds @16:20:56.57 ATV.dial :bleach @16:20:57.07 AS.seek 0.579ml @16:20:58.58 delay 5 seconds @16:21:03.61 CSR.select :air @16:21:03.75 CS.reconfigure CSfast @16:21:03.82 CS.seek 10ml @16:21:25.33 Pumping wcr puck from tube 2 up to 55psi... @16:21:25.35 CBV.dial SSV @16:21:26.84 CBV.dial between CSV and waste2b @16:21:27.99 CSV.dial between air and CTV @16:21:28.73 CS.seek 7.231ml @16:21:36.78 CTV.dial CSV,avoiding: :air @16:21:36.82 CSV.dial CTV @16:21:38.42 CS.reconfigure CSpressurize @16:21:38.50 CS.seek 0ml @16:21:57.83 CSV.dial :air @16:21:58.39 CS.reconfigure CSfast @16:21:58.47 CS.seek 10ml @16:22:25.43 CSV.dial between air and CTV @16:22:25.95 CS.seek 2.257ml @16:22:46.90 CTV.dial CSV,avoiding: :air @16:22:46.93 CSV.dial CTV @16:22:47.32 CS.reconfigure CSpressurize @16:22:47.40 CS.seek 0ml @16:22:49.83 delay 2:00 @16:22:51.98 delay 3 seconds @16:22:55.01 ATV.dial :air3 @16:22:55.60 delay 3 seconds @16:22:58.69 AS.seek 0.604ml @16:23:04.03 delay 3 seconds @16:23:07.06 ATV.dial :bleach @16:23:07.70 AS.seek 0.589ml @16:23:11.28 delay 3 seconds @16:23:14.31 ATV.dial :ASV @16:23:15.39 ASV.dial :wasteR @16:23:16.01 AS.seek 0ml @16:24:49.85 CBV.dial :waste2b @16:24:50.25 CS.reconfigure CSconfig @16:24:50.32 CS.seek :empty @16:24:55.67 delay 1:00 @16:25:14.77 delay 1:00 @16:25:55.70 CTV.dial :air,avoiding: :intake @16:25:57.34 Storing wcr puck in Collection Clamp to tube 1 @16:25:57.56 SC.reconfigure SCtight @16:25:57.63 SC.seek 1 @16:25:59.82 SC.reconfigure SCloose @16:26:00.08 Elbow.reconfigure TightElbow @16:26:00.16 Elbow.seek 1 @16:26:02.10 Forearm.reconfigure TightForearm @16:26:02.18 Forearm.seek 1 + 700 counts @16:26:03.27 SE.reconfigure SEpick @16:26:03.37 SE.seek beamBreak @16:26:06.53 SE.configure SEconfig @16:26:06.72 Elbow.reconfigure LimpElbow @16:26:06.79 Forearm.reconfigure LooseForearm @16:26:06.86 Forearm.seek 1 @16:26:07.22 Elbow.hold @16:26:07.32 Elbow.reconfigure TightElbow @16:26:07.45 SE.seek 1.07 Pucks below Top Plate @16:26:08.19 Forearm.force -20 @16:26:08.26 Forearm.seek 1 + 318 counts @16:26:08.64 Forearm.reconfigure TightForearm @16:26:08.71 Forearm.seek 1 + 700 counts @16:26:09.12 SE.seek 2.07 Pucks below Top Plate @16:26:09.87 Forearm.reconfigure LooseForearm @16:26:09.95 Forearm.seek :retract @16:26:10.92 Elbow.seek CC @16:26:12.70 Elbow.reconfigure LooseElbow @16:26:12.83 Forearm.seek CC @16:26:13.93 Hand.close @16:26:14.69 CC.seek :open @16:26:14.79 AFV.select :carrierSolution @16:26:14.91 delay 2 seconds @16:26:16.95 AS.reconfigure ASconfig @16:26:17.01 AS.seek 1ml @16:26:19.26 Forearm.seek :retract @16:26:20.30 Elbow.reconfigure TightElbow @16:26:20.38 Elbow.seek 1 @16:26:22.38 Elbow.reconfigure LimpElbow @16:26:22.46 Forearm.seek 1 @16:26:23.52 Elbow.hold @16:26:24.17 Elbow.reconfigure TightElbow @16:26:24.24 Hand.open @16:26:25.19 Forearm.force -20 @16:26:25.26 Forearm.seek 1 + 348 counts @16:26:25.62 Forearm.reconfigure TightForearm @16:26:25.69 Forearm.seek 1 + 700 counts @16:26:26.10 SE.seek :down @16:26:27.24 Forearm.reconfigure LooseForearm @16:26:27.32 Forearm.seek :retract @16:26:28.77 SE.coast @16:26:28.83 SC.coast @16:26:28.89 Starting finalPurge @16:26:28.91 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:26:29.02 Hand.open @16:26:29.46 Elbow.seek FlushPuck::Garage @16:26:31.54 Elbow.reconfigure LooseElbow @16:26:31.67 Forearm.seek :garage @16:26:32.74 Hand.close @16:26:33.69 Forearm.seek :retract @16:26:34.75 Elbow.reconfigure TightElbow @16:26:34.82 Elbow.seek CC @16:26:37.81 Elbow.reconfigure LooseElbow @16:26:37.94 Forearm.reconfigure TightForearm @16:26:38.01 Forearm.seek CC @16:26:39.40 CC.seek :closed @16:26:43.77 Collection Clamp onSpring/offSpring for 6/30 samples, on/off torque 2.39 @16:26:43.87 Hand.open @16:26:44.75 Forearm.reconfigure LooseForearm @16:26:44.83 Forearm.seek :retract @16:26:45.90 Flushing the Collection Valve::Manifold @16:26:46.07 CSV.dial CSR @16:26:46.67 Pulling 5ml of CSR.flush @16:26:46.68 CSR.select :flush @16:26:46.83 CS.seek 5ml @16:27:00.83 delay 5 seconds @16:27:05.86 CSR.select :air @16:27:06.05 CS.reconfigure CSfast @16:27:06.12 CS.seek 7.5ml @16:27:13.58 CSV.dial :waste @16:27:14.77 delay 1 second @16:27:15.12
CS.reconfigure CSconfig @16:27:15.19 CS.seek 0ml @16:27:15.79 AFV.select :holdingCoil @16:27:15.91 delay 1 second @16:27:16.94 ATV.dial ASV @16:27:16.95 ASV.dial :wasteR @16:27:16.99 AS.reconfigure ASslow1 @16:27:17.06 AS.seek 0ml @16:27:35.52
Flushing lower manifold's waste1 port @16:27:36.21 CSV.dial :flush @16:27:36.76 CS.reconfigure CSfast @16:27:36.83 CS.seek 5ml @16:27:50.71 delay 5 seconds @16:27:55.74 CSV.dial :air @16:27:56.36 CS.seek 7ml @16:28:02.74 CSV.dial CTV @16:28:02.78
CTV.dial CSV @16:28:04.43 CBV.dial :waste1 @16:28:05.34 CS.seek 0ml @16:28:24.46 Flushing lower manifold's waste2b port @16:28:24.62 CSV.dial :flush @16:28:25.51 CS.seek 5ml @16:28:39.52 delay 5 seconds @16:28:44.55 CSV.dial :air @16:28:45.18 CS.seek 7ml @16:28:51.30 CSV.dial CTV @16:28:51.34
CTV.dial CSV @16:28:51.88 CBV.dial :waste2b @16:28:52.81 CS.seek 0ml @16:29:11.86 Flushing upper manifold @16:29:12.03 CSV.dial :flush @16:29:12.92 CS.seek 5ml @16:29:27.02 delay 5 seconds @16:29:32.05 CSV.dial :air @16:29:32.68 CS.seek 7ml @16:29:38.80 CSV.dial CBV @16:29:38.84
CBV.dial CSV @16:29:40.13 CTV.dial :air @16:29:41.72 CS.seek 0ml @16:30:00.74 delay 7 seconds @16:30:07.78 CS.seek 3ml @16:30:16.55 CSV.dial :waste @16:30:17.11 CS.seek 0ml @16:30:25.90 CSV.dial :flush @16:30:26.44 CS.seek 3ml @16:30:35.30 delay 5 seconds @16:30:38.42 delay 20 seconds @16:30:40.33
CSV.dial :air @16:30:41.04 CS.seek 5ml @16:30:47.27 CSV.dial :air @16:30:47.30 CS.seek 0ml @16:30:58.45 ATV.dial :air3 @16:31:00.03 AS.seek 0.59ml @16:31:01.15
delay 7 seconds @16:31:08.19 CS.seek 3ml @16:31:17.02 CSV.dial :waste @16:31:17.89 CS.seek 0ml @16:31:26.65 Disinfecting external Intake with mfbkill @16:31:26.82 CSV.dial CSR @16:31:28.45 Pulling 8ml of CSR.mfbkill @16:31:28.47 CSR.select :mfbkill @16:31:28.67 CS.seek 8ml @16:31:50.46 delay 5 seconds @16:31:55.49 CSR.select :air @16:31:55.64 CS.seek 10ml @16:32:01.80 CSV.dial CBV @16:32:01.84
CBV.dial CSV @16:32:03.04 CTV.dial :intake @16:32:03.93 Intake.select :open @16:32:04.07 CS.seek 2ml @16:32:27.12 Intake.select :close @16:32:27.25 CTV.dial :air @16:32:28.16 CS.reconfigure CSconfig @16:32:28.23 CS.seek 0ml @16:32:34.43 Flushing lower manifold's waste1 port @16:32:34.45 CSV.dial CSR @16:32:35.86 Pulling 4ml of CSR.mfbkill @16:32:35.87 CSR.select :mfbkill @16:32:36.02 CS.seek 4ml @16:32:48.12 delay 5 seconds @16:32:53.15 CSR.select :air @16:32:53.30 CS.reconfigure CSfast @16:32:53.37 CS.seek 10ml @16:32:59.03 delay 3 seconds @16:33:02.06 ATV.dial :ASV @16:33:03.55 ASV.dial :wasteR @16:33:03.66 AS.seek 0.09ml @16:33:09.91 CSV.dial CTV @16:33:09.94
CTV.dial CSV @16:33:11.60 CBV.dial :waste1 @16:33:12.16 CS.seek 0ml @16:33:38.96 Flushing lower manifold's waste2b port @16:33:38.98 CSV.dial CSR @16:33:39.61 Pulling 4ml of CSR.mfbkill @16:33:39.62 CSR.select :mfbkill @16:33:39.78 CS.reconfigure CSconfig @16:33:39.85 CS.seek 4ml @16:33:51.30 delay 5 seconds @16:33:56.33 CSR.select :air @16:33:56.48 CS.reconfigure CSfast @16:33:56.55 CS.seek 10ml @16:34:13.18 CSV.dial CTV @16:34:13.22
CTV.dial CSV @16:34:13.77 CBV.dial :waste2b @16:34:14.70 CS.seek 0ml @16:34:41.52 Killing upper manifold @16:34:41.54 CSV.dial CSR @16:34:42.17 Pulling 4ml of CSR.mfbkill @16:34:42.18 CSR.select :mfbkill @16:34:42.34 CS.reconfigure CSconfig @16:34:42.41 CS.seek 4ml @16:34:44.52 delay 30 seconds @16:34:53.68
delay 5 seconds @16:34:58.71 CSR.select :air @16:34:58.86 CS.reconfigure CSfast @16:34:58.93 CS.seek 10ml @16:35:14.55 ATV.dial :extSrc @16:35:15.35 CSV.dial CBV @16:35:15.39
CBV.dial CSV @16:35:15.53 delay 3 seconds @16:35:16.76
CTV.dial :air @16:35:18.33 CS.seek 0ml @16:35:18.55 ATV.dial :ElutionWater @16:35:19.72 AS.seek 0.12ml @16:35:26.13 ATV.dial :air3 @16:35:26.79 AS.seek 0.13ml @16:35:29.17 delay 2 seconds @16:35:31.19 ATV.dial :bleach @16:35:31.70 AS.seek 0.23ml @16:35:45.15
delay 7 seconds @16:35:52.17 delay 3 seconds @16:35:52.21
CS.seek 3ml @16:35:55.20 ATV.dial :air3 @16:35:55.79 delay 3 seconds @16:35:58.87 AS.seek 0.255ml @16:36:01.02
CSV.dial :waste @16:36:01.57 CS.seek 0ml @16:36:04.24 delay 3 seconds @16:36:07.26 ATV.dial :bleach @16:36:07.89 AS.seek 0.24ml @16:36:10.51
Flushing upper manifold @16:36:11.34 CSV.dial :flush @16:36:11.47 delay 3 seconds @16:36:12.22
CS.seek 5ml @16:36:14.57 ATV.dial :ASV @16:36:15.90 ASV.dial :SPEdelivery @16:36:16.67 AFV.select :carrierSolution @16:36:17.12 delay 2 seconds @16:36:19.18 AS.reconfigure ASconfig @16:36:19.24 AS.seek 0.5ml @16:36:26.43
delay 5 seconds @16:36:31.46 CSV.dial :air @16:36:32.06 CS.seek 7ml @16:36:34.50 delay 1 second @16:36:35.52 AFV.select :holdingCoil @16:36:35.69 AS.reconfigure ASslow1 @16:36:35.75 AS.seek 0.17ml @16:36:38.18 CSV.dial CBV @16:36:38.22
CBV.dial CSV @16:36:39.99 CTV.dial :air @16:36:40.02 CS.seek 0ml @16:36:59.27 delay 7 seconds @16:37:06.31 CS.seek 3ml @16:37:15.02 CSV.dial :waste @16:37:15.57 CS.seek 0ml @16:37:24.47 CSV.dial :flush @16:37:25.00 CS.seek 3ml @16:37:33.87 delay 5 seconds @16:37:38.90 CSV.dial :air @16:37:39.50 CS.seek 5.001ml @16:37:42.45 SPE.hold 2:46:40,95C @16:37:42.50 delay 3:00 @16:37:45.68
CSV.dial :air @16:37:45.71 CS.seek 0ml @16:38:00.18 delay 7 seconds @16:38:07.22 CS.seek 3ml @16:38:16.46 CSV.dial :waste @16:38:17.39 CS.seek 0ml @16:38:26.62 Flushing lower manifold's waste1 port @16:38:26.78 CSV.dial :flush @16:38:27.35 CS.seek 5ml @16:38:41.96 delay 5 seconds @16:38:46.99 CSV.dial :air @16:38:47.62 CS.seek 7ml @16:38:53.99 CSV.dial CTV @16:38:54.03
CTV.dial CSV @16:38:55.74 CBV.dial :waste1 @16:38:56.30 CS.seek 0ml @16:39:16.15 Flushing lower manifold's waste2b port @16:39:16.31 CSV.dial :flush @16:39:17.24 CS.seek 5ml @16:39:31.74 delay 5 seconds @16:39:36.77 CSV.dial :air @16:39:37.42 CS.seek 7ml @16:39:43.90 CSV.dial CTV @16:39:43.94
CTV.dial CSV @16:39:44.48 CBV.dial :waste2b @16:39:45.44 CS.seek 0ml @16:40:05.46 Disinfecting external Intake with kill @16:40:05.62 CSV.dial CSR @16:40:06.22 Pulling 7.999ml of CSR.kill @16:40:06.23 CSR.select :kill @16:40:06.39 CS.seek 8ml @16:40:29.09 delay 5 seconds @16:40:34.12 CSR.select :air @16:40:34.27 CS.seek 10ml @16:40:40.65 CSV.dial CBV @16:40:40.69
CBV.dial CSV @16:40:42.01 CTV.dial :intake @16:40:42.53 AFV.select :carrierSolution @16:40:42.65 delay 2 seconds @16:40:42.93
Intake.select :open @16:40:43.07 CS.seek 2ml @16:40:44.69 AS.reconfigure ASconfig @16:40:44.75 AS.seek 1ml @16:41:07.15
Intake.select :close @16:41:07.28 CTV.dial :air @16:41:08.22 CS.reconfigure CSconfig @16:41:08.28 CS.seek 0ml @16:41:14.65 CSV.dial CSR @16:41:16.00 Pulling 5ml of CSR.flush @16:41:16.01 CSR.select :flush @16:41:16.17 CS.seek 5ml @16:41:30.81 delay 5 seconds @16:41:32.78 delay 1 second @16:41:33.80 AFV.select :holdingCoil @16:41:33.91 delay 1 second @16:41:34.94 ASV.dial :wasteD @16:41:35.84
CSR.select :air @16:41:35.99 CS.seek 10ml @16:41:36.09 AS.seek 0ml @16:41:50.68
CSV.dial CBV @16:41:51.97 CS.seek 0ml @16:42:20.71 Garaging FlushPuck in Collection Clamp @16:42:20.82 Elbow.reconfigure TightElbow @16:42:20.89 Elbow.seek CC @16:42:21.16 Elbow.reconfigure LooseElbow @16:42:21.29 Forearm.seek CC @16:42:22.38 Hand.close @16:42:23.21 CC.seek :open @16:42:27.67 Forearm.seek :retract @16:42:28.74 Elbow.reconfigure TightElbow @16:42:28.82 Elbow.seek :garage @16:42:32.03 Elbow.reconfigure LooseElbow @16:42:32.16 Forearm.reconfigure TightForearm @16:42:32.22 Forearm.seek :garage @16:42:33.41 Hand.open @16:42:33.91 Priming Eluate @16:42:33.92 AFV.select :carrierSolution @16:42:34.05 delay 2 seconds @16:42:34.28
Forearm.reconfigure LooseForearm @16:42:34.34 Forearm.seek :retract @16:42:35.41 Starting initialPurge @16:42:35.43 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @16:42:35.53 Hand.open @16:42:35.89 Elbow.reconfigure TightElbow @16:42:35.96 Elbow.seek FlushPuck::Garage @16:42:36.16 AS.seek 0.5ml @16:42:36.25
Elbow.reconfigure LooseElbow @16:42:36.38 Forearm.seek :garage @16:42:37.46 Hand.close @16:42:38.39 Forearm.seek :retract @16:42:39.52 Elbow.reconfigure TightElbow @16:42:39.59 Elbow.seek CC @16:42:42.56 Elbow.reconfigure LooseElbow @16:42:42.69 Forearm.reconfigure TightForearm @16:42:42.76 Forearm.seek CC @16:42:44.18 CC.seek :closed @16:42:48.53 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.50 @16:42:48.63 Hand.open @16:42:49.52 Forearm.reconfigure LooseForearm @16:42:49.59 Forearm.seek :retract @16:42:50.68 Flushing the Collection Valve::Manifold @16:42:50.84 CSV.dial CSR @16:42:52.16 Pulling 5ml of CSR.flush @16:42:52.17 CSR.select :flush @16:42:52.33 CS.seek 5ml @16:43:05.19 delay 1 second @16:43:06.21 AFV.select :holdingCoil @16:43:06.32 ATV.dial ASV @16:43:06.34 ASV.dial :SPErecovery @16:43:06.56
delay 5 seconds @16:43:06.98 Swish #1 of 2 @16:43:07.08 AS.reconfigure ASslow0 @16:43:07.15 AS.seek 0.6ml @16:43:11.58
CSR.select :air @16:43:11.77 CS.reconfigure CSfast @16:43:11.83 CS.seek 7.5ml @16:43:17.63 AS.seek 0.5ml @16:43:19.68
CSV.dial :waste @16:43:21.26 CS.reconfigure CSconfig @16:43:21.33 CS.seek 0ml @16:43:28.00 Swish #2 of 2 @16:43:28.10 AS.seek 0.6ml @16:43:38.60 AS.seek 0.5ml @16:43:42.56
Purging external Intake @16:43:42.72 CSV.dial :flush @16:43:43.27 CS.reconfigure CSfast @16:43:43.34 CS.seek 10ml @16:43:48.98 delay 3 seconds @16:43:52.07 AS.reconfigure ASslow1 @16:43:52.14 AS.seek 0.684ml @16:44:10.75
delay 5 seconds @16:44:15.78 CSV.dial CBV @16:44:15.82
CBV.dial CSV @16:44:16.73 CTV.dial :intake @16:44:17.65 Intake.select :open @16:44:17.79 CS.seek 0ml @16:44:29.44 delay 3 seconds @16:44:32.47 Eluate primed @16:44:32.48 ATV.dial :waste2 @16:44:33.17 AS.reconfigure ASnorm @16:44:33.24 AS.seek 0ml @16:44:46.72
Intake.select :close @16:44:46.85 CTV.dial :air @16:44:47.76 Flushing upper manifold @16:44:47.92 CSV.dial :flush @16:44:48.80 CS.seek 5ml @16:45:03.03 delay 5 seconds @16:45:08.09 SPE.coast @16:45:08.16
CSV.dial :air @16:45:08.25 ASV.dial :SPErecovery @16:45:08.27 Setting up PCR for OM -- writing /var/log/esp/OM20jul1316h.pcr @16:45:08.30 Building PCR Slug from ASV with MM3 mix, PP5 primer @16:45:08.34 Priming with carrier @16:45:08.36 ATV.dial ARV @16:45:08.75
CS.seek 7ml @16:45:09.38 ARV.dial :carrier @16:45:09.77 AS.seek 0.5ml @16:45:14.87 CSV.dial CBV @16:45:14.91
CBV.dial CSV @16:45:16.11 CTV.dial :air @16:45:16.14 CS.seek 0ml @16:45:34.90 delay 7 seconds @16:45:35.38 AFV.select :carrierSolution @16:45:35.51 delay 2 seconds @16:45:37.55 AS.reconfigure ASconfig @16:45:37.62 AS.seek 1ml @16:45:41.93
CS.seek 3ml @16:45:50.53 CSV.dial :waste @16:45:51.20 CS.seek 0ml @16:45:59.93 CSV.dial :flush @16:46:00.48 CS.seek 3ml @16:46:06.67 delay 1 second @16:46:07.70 AFV.select :holdingCoil @16:46:07.82 delay 1 second @16:46:08.85 ATV.dial :waste @16:46:09.15
delay 5 seconds @16:46:10.42 AS.reconfigure ASnorm @16:46:10.49 AS.seek :empty @16:46:14.17
CSV.dial :air @16:46:14.77 CS.seek 5ml @16:46:20.90 CSV.dial :air @16:46:20.93 CS.seek 0ml @16:46:34.56 delay 7 seconds @16:46:41.60 CS.seek 3ml @16:46:50.22 CSV.dial :waste @16:46:51.10 CS.seek 0ml @16:46:59.78 Garaging FlushPuck in Collection Clamp @16:46:59.88 Elbow.reconfigure TightElbow @16:46:59.95 Elbow.seek CC @16:47:00.21 Elbow.reconfigure LooseElbow @16:47:00.34 Forearm.seek CC @16:47:01.23 AFV.select :carrierSolution @16:47:01.36 delay 2 seconds @16:47:01.43
Hand.close @16:47:02.21 CC.seek :open @16:47:03.39 AS.reconfigure ASconfig @16:47:03.46 AS.seek 0.5ml @16:47:06.63
Forearm.seek :retract @16:47:07.67 Elbow.reconfigure TightElbow @16:47:07.74 Elbow.seek :garage @16:47:11.56 Elbow.reconfigure LooseElbow @16:47:11.69 Forearm.reconfigure TightForearm @16:47:11.75 Forearm.seek :garage @16:47:12.95 Hand.open @16:47:13.77 Forearm.reconfigure LooseForearm @16:47:13.84 Forearm.seek :retract @16:47:14.93 Completed [coreNEG] begun 15:03:27 @16:47:14.95 Extending InvasivePostCoreNeg until completion of MFBPCR @16:47:32.59 delay 1 second @16:47:33.62 AFV.select :holdingCoil @16:47:33.74 Building Slug @16:47:33.76 ATV.dial :ARV @16:47:35.45 delay 2 seconds @16:47:37.48 ARV.dial :air1 @16:47:37.90 delay 2 seconds @16:47:39.99 AS.reconfigure ASslow2 @16:47:40.07 AS.seek 0.506ml @16:47:46.47 delay 2 seconds @16:47:48.50 ARV.dial :MM3 @16:47:49.59 delay 2 seconds @16:47:51.62 Adding MM3 @16:47:51.73 AS.seek 0.524ml @16:48:10.20 delay 25 seconds @16:48:35.23 ARV.dial :PP5 @16:48:35.65 Adding PP5 @16:48:35.66 delay 2 seconds @16:48:37.75 AS.seek 0.53ml @16:48:43.94 delay 10 seconds @16:48:53.97 ARV.dial :air3 @16:48:54.40 adding ARV air @16:48:54.41 delay 2 seconds @16:48:56.50 AS.seek 0.536ml @16:49:02.70 delay 5 seconds @16:49:07.73 ARV.dial :carrier @16:49:09.05 delay 7 seconds @16:49:16.14 AS.seek 0.594ml @16:50:14.72 delay 5 seconds @16:50:19.75 ATV.dial :ASV @16:50:20.19 delay 2 seconds @16:50:22.28 AS.seek 0.6ml @16:50:28.72 delay 4 seconds @16:50:32.75 ATV.dial ARV @16:50:33.33 delay 2 seconds @16:50:35.36 ARV.dial :carrier @16:50:35.38 delay 2 seconds @16:50:37.47 AS.seek 0.65ml @16:51:28.17 delay 4 seconds @16:51:32.20 ATV.dial PCR @16:51:32.83 ASV.dial :waste1 @16:51:33.64 ARV.dial :air1 @16:51:34.06 Positioning Slug by 0.324ml @16:51:34.17 AS.seek 0.326ml @16:57:00.17 delay 5 seconds @16:57:05.20 ATV.dial ARV @16:57:06.00 ASV.dial :PCRlock @16:57:06.41 delay 5 seconds @16:57:11.44 Running OM Profile60C350 thermocycling @16:57:11.46 PCR.program Profile60C350 @16:57:11.60 Profile60C350 should complete in < 2:02:45 @16:57:11.62 PCR.run 2:38:26.25 @18:48:01.80 PCR.off @18:48:03.10 PCR for OM completed -- closed /var/log/esp/OM20jul1316h.pcr @18:48:03.19 Cleaning PCR @18:48:03.20 AFV.select :carrierSolution @18:48:03.34 delay 2 seconds @18:48:05.38 AS.reconfigure ASconfig @18:48:05.44 AS.seek 0.5ml @18:48:15.85 delay 1 second @18:48:16.87 AFV.select :holdingCoil @18:48:16.99 ASV.dial :waste1 @18:48:17.40 ATV.dial :air3 @18:48:18.73 AS.reconfigure ASslow1 @18:48:18.80 AS.seek 0.51ml @18:48:21.10 delay 2 seconds @18:48:23.12 ATV.dial :bleach @18:48:23.76 AS.seek 0.81ml @18:49:24.34 delay 3 seconds @18:49:27.37 ATV.dial :air3 @18:49:27.93 delay 3 seconds @18:49:31.02 AS.seek 0.835ml @18:49:36.37 delay 3 seconds @18:49:39.40 ATV.dial :bleach @18:49:40.03 AS.seek 0.82ml @18:49:43.34 delay 3 seconds @18:49:46.37 ARV.dial :waste @18:49:47.02 delay 3 seconds @18:49:50.05 ATV.dial :ARV @18:49:50.98 AS.reconfigure ASconfig @18:49:51.05 AS.seek 0.47ml @18:50:11.48 AFV.select :carrierSolution @18:50:11.61 delay 2 seconds @18:50:13.65 AS.seek 1ml @18:50:44.46 delay 1 second @18:50:45.48 AFV.select :holdingCoil @18:50:45.60 delay 1 second @18:50:46.64 AS.reconfigure ASfast @18:50:46.70 AS.seek 0ml @18:51:14.07 AFV.select :carrierSolution @18:51:14.20 delay 2 seconds @18:51:16.24 AS.reconfigure ASconfig @18:51:16.30 AS.seek 1ml @18:52:14.07 delay 1 second @18:52:15.09 AFV.select :holdingCoil @18:52:15.21 delay 1 second @18:52:16.25 AS.reconfigure ASfast @18:52:16.35 AS.seek 0ml @18:52:43.77 AFV.select :carrierSolution @18:52:43.90 delay 2 seconds @18:52:45.94 AS.reconfigure ASconfig @18:52:46.00 AS.seek 1ml @18:53:43.80 delay 1 second @18:53:44.82 AFV.select :holdingCoil @18:53:44.94 delay 1 second @18:53:45.98 AS.reconfigure ASfast @18:53:46.04 AS.seek 0ml @18:54:13.41 ARV.dial between carrier and air1 @18:54:13.95 ATV.dial :air3 @18:54:15.28 AS.reconfigure ASslow1 @18:54:15.35 AS.seek 0.01ml @18:54:17.71 delay 2 seconds @18:54:19.73 ATV.dial :bleach @18:54:20.39 AS.seek 0.81ml @18:57:01.60 delay 3 seconds @18:57:04.63 ATV.dial :air3 @18:57:05.21 delay 3 seconds @18:57:08.30 AS.seek 0.835ml @18:57:13.62 delay 3 seconds @18:57:16.65 ATV.dial :bleach @18:57:17.30 AS.seek 0.82ml @18:57:20.63 delay 3 seconds @18:57:23.66 ATV.dial PCR @18:57:24.08 AS.reconfigure ASconfig @18:57:24.14 AS.seek :empty @18:58:11.65 PCR.program :bleachProfile @18:58:11.72 PCR program should complete in < 2:45 @18:58:11.73 PCR.run 10:00 @19:01:01.45 PCR.off @19:01:01.49 AFV.select :carrierSolution @19:01:01.61 delay 2 seconds @19:01:03.65 AS.seek 1ml @19:02:01.49 delay 1 second @19:02:02.51 AFV.select :holdingCoil @19:02:02.63 delay 1 second @19:02:03.67 AS.reconfigure ASfast @19:02:03.73 AS.seek 0ml @19:02:31.12 delay 3 seconds @19:02:34.15 AFV.select :carrierSolution @19:02:34.27 delay 2 seconds @19:02:36.31 AS.reconfigure ASconfig @19:02:36.38 AS.seek 1ml @19:03:34.19 delay 1 second @19:03:35.21 AFV.select :holdingCoil @19:03:35.33 delay 1 second @19:03:36.37 AS.reconfigure ASfast @19:03:36.43 AS.seek 0ml @19:04:03.80 delay 3 seconds @19:04:06.83 AFV.select :carrierSolution @19:04:07.45 delay 2 seconds @19:04:09.56 AS.reconfigure ASconfig @19:04:09.62 AS.seek 1ml @19:05:07.43 delay 1 second @19:05:08.45 AFV.select :holdingCoil @19:05:08.57 delay 1 second @19:05:09.61 AS.reconfigure ASfast @19:05:09.67 AS.seek 0ml @19:05:37.13 delay 3 seconds @19:05:40.16 AFV.select :carrierSolution @19:05:40.28 delay 2 seconds @19:05:42.33 AS.reconfigure ASconfig @19:05:42.39 AS.seek 1ml @19:06:40.18 delay 1 second @19:06:41.20 AFV.select :holdingCoil @19:06:41.32 delay 1 second @19:06:42.36 AS.reconfigure ASfast @19:06:42.42 AS.seek 0ml @19:07:09.80 delay 3 seconds @19:07:12.83 AFV.select :carrierSolution @19:07:12.95 delay 2 seconds @19:07:14.99 AS.reconfigure ASconfig @19:07:15.06 AS.seek 1ml @19:08:12.83 delay 1 second @19:08:13.85 AFV.select :holdingCoil @19:08:13.97 delay 1 second @19:08:15.01 AS.reconfigure ASnorm @19:08:15.07 AS.seek 0ml @19:09:05.82 Setting up PCR for ML -- writing /var/log/esp/ML20jul1319h.pcr @19:09:05.84 Building PCR Slug from ASV with MM2 mix, PP4 primer @19:09:05.86 Priming with carrier @19:09:05.89 ATV.dial ARV @19:09:06.65 ARV.dial :carrier @19:09:07.04 AS.seek 0.5ml @19:09:32.62 AFV.select :carrierSolution @19:09:32.75 delay 2 seconds @19:09:34.79 AS.reconfigure ASconfig @19:09:34.86 AS.seek 1ml @19:10:03.90 delay 1 second @19:10:04.93 AFV.select :holdingCoil @19:10:05.05 delay 1 second @19:10:06.08 ATV.dial :waste @19:10:07.80 AS.reconfigure ASnorm @19:10:07.87 AS.seek :empty @19:10:58.63 AFV.select :carrierSolution @19:10:58.76 delay 2 seconds @19:11:00.80 AS.reconfigure ASconfig @19:11:00.86 AS.seek 0.5ml @19:11:29.99 delay 1 second @19:11:31.01 AFV.select :holdingCoil @19:11:31.14 Building Slug @19:11:31.15 ATV.dial :ARV @19:11:32.83 delay 2 seconds @19:11:34.86 ARV.dial :air1 @19:11:35.28 delay 2 seconds @19:11:37.37 AS.reconfigure ASslow2 @19:11:37.45 AS.seek 0.506ml @19:11:43.68 delay 2 seconds @19:11:45.70 ARV.dial :MM2 @19:11:47.23 delay 2 seconds @19:11:49.26 Adding MM2 @19:11:49.37 AS.seek 0.524ml @19:12:07.63 delay 25 seconds @19:12:32.66 ARV.dial :PP4 @19:12:33.08 Adding PP4 @19:12:33.09 delay 2 seconds @19:12:35.18 AS.seek 0.53ml @19:12:41.55 delay 10 seconds @19:12:51.58 ARV.dial :air3 @19:12:51.99 adding ARV air @19:12:52.01 delay 2 seconds @19:12:54.10 AS.seek 0.536ml @19:13:00.37 delay 5 seconds @19:13:05.40 ARV.dial :carrier @19:13:06.69 delay 7 seconds @19:13:13.78 AS.seek 0.594ml @19:14:12.26 delay 5 seconds @19:14:17.29 ATV.dial :ASV @19:14:17.71 delay 2 seconds @19:14:19.80 AS.seek 0.6ml @19:14:26.01 delay 4 seconds @19:14:30.04 ATV.dial ARV @19:14:30.62 delay 2 seconds @19:14:32.65 ARV.dial :carrier @19:14:32.67 delay 2 seconds @19:14:34.76 AS.seek 0.65ml @19:15:25.26 delay 4 seconds @19:15:29.29 ATV.dial PCR @19:15:29.93 ASV.dial :waste1 @19:15:29.95 ARV.dial :air1 @19:15:30.39 Positioning Slug by 0.324ml @19:15:30.49 AS.seek 0.326ml @19:20:56.65 delay 5 seconds @19:21:01.68 ATV.dial ARV @19:21:02.48 ASV.dial :PCRlock @19:21:02.90 delay 5 seconds @19:21:07.93 Running ML Profile60C350 thermocycling @19:21:07.95 PCR.program Profile60C350 @19:21:08.08 Profile60C350 should complete in < 2:02:45 @19:21:08.09 PCR.run 2:38:26.25 @21:11:55.88 PCR.off @21:11:57.65 PCR for ML completed -- closed /var/log/esp/ML20jul1319h.pcr @21:11:57.74 Cleaning PCR @21:11:57.75 AFV.select :carrierSolution @21:11:57.88 delay 2 seconds @21:11:59.92 AS.reconfigure ASconfig @21:11:59.98 AS.seek 0.5ml @21:12:10.33 delay 1 second @21:12:11.35 AFV.select :holdingCoil @21:12:11.47 ASV.dial :waste1 @21:12:11.89 ATV.dial :air3 @21:12:13.23 AS.reconfigure ASslow1 @21:12:13.30 AS.seek 0.51ml @21:12:15.59 delay 2 seconds @21:12:17.61 ATV.dial :bleach @21:12:18.24 AS.seek 0.81ml @21:13:18.86 delay 3 seconds @21:13:21.89 ATV.dial :air3 @21:13:22.45 delay 3 seconds @21:13:25.54 AS.seek 0.835ml @21:13:30.86 delay 3 seconds @21:13:33.90 ATV.dial :bleach @21:13:34.54 AS.seek 0.82ml @21:13:37.87 delay 3 seconds @21:13:40.90 ARV.dial :waste @21:13:41.51 delay 3 seconds @21:13:44.54 ATV.dial :ARV @21:13:45.48 AS.reconfigure ASconfig @21:13:45.55 AS.seek 0.47ml @21:14:05.98 AFV.select :carrierSolution @21:14:06.11 delay 2 seconds @21:14:08.15 AS.seek 1ml @21:14:38.94 delay 1 second @21:14:39.96 AFV.select :holdingCoil @21:14:40.08 delay 1 second @21:14:41.12 AS.reconfigure ASfast @21:14:41.18 AS.seek 0ml @21:15:08.58 AFV.select :carrierSolution @21:15:08.71 delay 2 seconds @21:15:10.75 AS.reconfigure ASconfig @21:15:10.81 AS.seek 1ml @21:16:08.61 delay 1 second @21:16:09.63 AFV.select :holdingCoil @21:16:09.75 delay 1 second @21:16:10.79 AS.reconfigure ASfast @21:16:10.85 AS.seek 0ml @21:16:38.22 AFV.select :carrierSolution @21:16:38.35 delay 2 seconds @21:16:40.39 AS.reconfigure ASconfig @21:16:40.45 AS.seek 1ml @21:17:38.26 delay 1 second @21:17:39.28 AFV.select :holdingCoil @21:17:39.40 delay 1 second @21:17:40.44 AS.reconfigure ASfast @21:17:40.50 AS.seek 0ml @21:18:07.87 ARV.dial between carrier and air1 @21:18:08.39 ATV.dial :air3 @21:18:09.73 AS.reconfigure ASslow1 @21:18:09.80 AS.seek 0.01ml @21:18:12.17 delay 2 seconds @21:18:14.19 ATV.dial :bleach @21:18:14.85 AS.seek 0.81ml @21:20:56.00 delay 3 seconds @21:20:59.03 ATV.dial :air3 @21:20:59.61 delay 3 seconds @21:21:02.70 AS.seek 0.835ml @21:21:07.95 delay 3 seconds @21:21:10.98 ATV.dial :bleach @21:21:11.63 AS.seek 0.82ml @21:21:14.94 delay 3 seconds @21:21:17.97 ATV.dial PCR @21:21:18.40 AS.reconfigure ASconfig @21:21:18.46 AS.seek :empty @21:22:05.95 PCR.program :bleachProfile @21:22:06.02 PCR program should complete in < 2:45 @21:22:06.03 PCR.run 10:00 @21:24:55.75 PCR.off @21:24:55.79 AFV.select :carrierSolution @21:24:55.91 delay 2 seconds @21:24:57.95 AS.seek 1ml @21:25:55.80 delay 1 second @21:25:56.82 AFV.select :holdingCoil @21:25:56.94 delay 1 second @21:25:57.98 AS.reconfigure ASfast @21:25:58.04 AS.seek 0ml @21:26:26.00 delay 3 seconds @21:26:29.03 AFV.select :carrierSolution @21:26:29.15 delay 2 seconds @21:26:31.19 AS.reconfigure ASconfig @21:26:31.26 AS.seek 1ml @21:27:29.05 delay 1 second @21:27:30.07 AFV.select :holdingCoil @21:27:30.19 delay 1 second @21:27:31.23 AS.reconfigure ASfast @21:27:31.29 AS.seek 0ml @21:27:58.76 delay 3 seconds @21:28:01.79 AFV.select :carrierSolution @21:28:01.91 delay 2 seconds @21:28:03.95 AS.reconfigure ASconfig @21:28:04.02 AS.seek 1ml @21:29:01.83 delay 1 second @21:29:02.85 AFV.select :holdingCoil @21:29:02.98 delay 1 second @21:29:04.02 AS.reconfigure ASfast @21:29:04.08 AS.seek 0ml @21:29:31.45 delay 3 seconds @21:29:34.48 AFV.select :carrierSolution @21:29:34.60 delay 2 seconds @21:29:36.64 AS.reconfigure ASconfig @21:29:36.71 AS.seek 1ml @21:30:34.48 delay 1 second @21:30:35.50 AFV.select :holdingCoil @21:30:35.62 delay 1 second @21:30:36.66 AS.reconfigure ASfast @21:30:36.72 AS.seek 0ml @21:31:04.09 delay 3 seconds @21:31:07.12 AFV.select :carrierSolution @21:31:07.24 delay 2 seconds @21:31:09.28 AS.reconfigure ASconfig @21:31:09.35 AS.seek 1ml @21:32:07.12 delay 1 second @21:32:08.14 AFV.select :holdingCoil @21:32:08.26 delay 1 second @21:32:09.30 AS.reconfigure ASnorm @21:32:09.36 AS.seek 0ml @21:33:00.11 Setting up PCR for CG -- writing /var/log/esp/CG20jul1321h.pcr @21:33:00.13 Building PCR Slug from ASV with MM1 mix, PP2 primer @21:33:00.16 Priming with carrier @21:33:00.18 ATV.dial ARV @21:33:00.84 ARV.dial :carrier @21:33:01.22 AS.seek 0.5ml @21:33:26.79 AFV.select :carrierSolution @21:33:26.92 delay 2 seconds @21:33:28.96 AS.reconfigure ASconfig @21:33:29.03 AS.seek 1ml @21:33:58.08 delay 1 second @21:33:59.10 AFV.select :holdingCoil @21:33:59.22 delay 1 second @21:34:00.25 ATV.dial :waste @21:34:01.98 AS.reconfigure ASnorm @21:34:02.05 AS.seek :empty @21:34:52.81 AFV.select :carrierSolution @21:34:52.94 delay 2 seconds @21:34:54.98 AS.reconfigure ASconfig @21:34:55.04 AS.seek 0.5ml @21:35:24.19 delay 1 second @21:35:25.21 AFV.select :holdingCoil @21:35:25.33 Building Slug @21:35:25.35 ATV.dial :ARV @21:35:27.04 delay 2 seconds @21:35:29.07 ARV.dial :air1 @21:35:29.50 delay 2 seconds @21:35:31.57 AS.reconfigure ASslow2 @21:35:31.64 AS.seek 0.506ml @21:35:37.86 delay 2 seconds @21:35:39.88 ARV.dial :MM1 @21:35:40.50 delay 2 seconds @21:35:42.52 Adding MM1 @21:35:42.98 AS.seek 0.524ml @21:36:01.45 delay 25 seconds @21:36:26.48 ARV.dial :PP2 @21:36:26.89 Adding PP2 @21:36:26.91 delay 2 seconds @21:36:29.00 AS.seek 0.53ml @21:36:35.28 delay 10 seconds @21:36:45.31 ARV.dial :air2 @21:36:45.70 adding ARV air @21:36:45.72 delay 2 seconds @21:36:47.82 AS.seek 0.536ml @21:36:54.06 delay 5 seconds @21:36:59.09 ARV.dial :carrier @21:37:00.40 delay 7 seconds @21:37:07.49 AS.seek 0.594ml @21:38:05.98 delay 5 seconds @21:38:11.01 ATV.dial :ASV @21:38:11.44 delay 2 seconds @21:38:13.53 AS.seek 0.6ml @21:38:19.73 delay 4 seconds @21:38:23.76 ATV.dial ARV @21:38:24.33 delay 2 seconds @21:38:26.36 ARV.dial :carrier @21:38:26.38 delay 2 seconds @21:38:28.47 AS.seek 0.65ml @21:39:18.95 delay 4 seconds @21:39:22.98 ATV.dial PCR @21:39:23.62 ASV.dial :waste1 @21:39:23.64 ARV.dial :air1 @21:39:24.08 Positioning Slug by 0.324ml @21:39:24.18 AS.seek 0.326ml @21:44:50.81 delay 5 seconds @21:44:55.84 ATV.dial ARV @21:44:56.75 ASV.dial :PCRlock @21:44:57.17 delay 5 seconds @21:45:02.20 Running CG Profile60C300 thermocycling @21:45:02.22 PCR.program Profile60C300 @21:45:02.34 Profile60C300 should complete in < 1:59:25 @21:45:02.36 PCR.run 2:34:16.25 @23:34:30.01 PCR.off @23:34:31.30 PCR for CG completed -- closed /var/log/esp/CG20jul1321h.pcr @23:34:31.39 Cleaning PCR @23:34:31.40 AFV.select :carrierSolution @23:34:31.54 delay 2 seconds @23:34:33.58 AS.reconfigure ASconfig @23:34:33.64 AS.seek 0.5ml @23:34:43.94 delay 1 second @23:34:44.96 AFV.select :holdingCoil @23:34:45.08 ASV.dial :waste1 @23:34:45.49 ATV.dial :air3 @23:34:46.83 AS.reconfigure ASslow1 @23:34:46.90 AS.seek 0.51ml @23:34:49.20 delay 2 seconds @23:34:51.23 ATV.dial :bleach @23:34:51.89 AS.seek 0.81ml @23:35:52.48 delay 3 seconds @23:35:55.51 ATV.dial :air3 @23:35:56.08 delay 3 seconds @23:35:59.17 AS.seek 0.835ml @23:36:04.48 delay 3 seconds @23:36:07.51 ATV.dial :bleach @23:36:08.15 AS.seek 0.82ml @23:36:11.48 delay 3 seconds @23:36:14.51 ARV.dial :waste @23:36:15.16 delay 3 seconds @23:36:18.19 ATV.dial :ARV @23:36:19.12 AS.reconfigure ASconfig @23:36:19.19 AS.seek 0.47ml @23:36:39.62 AFV.select :carrierSolution @23:36:39.75 delay 2 seconds @23:36:41.79 AS.seek 1ml @23:37:12.54 delay 1 second @23:37:13.56 AFV.select :holdingCoil @23:37:13.68 delay 1 second @23:37:14.72 AS.reconfigure ASfast @23:37:14.78 AS.seek 0ml @23:37:42.13 AFV.select :carrierSolution @23:37:42.26 delay 2 seconds @23:37:44.30 AS.reconfigure ASconfig @23:37:44.36 AS.seek 1ml @23:38:42.13 delay 1 second @23:38:43.15 AFV.select :holdingCoil @23:38:43.27 delay 1 second @23:38:44.31 AS.reconfigure ASfast @23:38:44.37 AS.seek 0ml @23:39:11.74 AFV.select :carrierSolution @23:39:11.87 delay 2 seconds @23:39:13.91 AS.reconfigure ASconfig @23:39:13.97 AS.seek 1ml @23:40:11.76 delay 1 second @23:40:12.78 AFV.select :holdingCoil @23:40:12.90 delay 1 second @23:40:13.94 AS.reconfigure ASfast @23:40:14.00 AS.seek 0ml @23:40:41.38 ARV.dial between carrier and air1 @23:40:41.91 ATV.dial :air3 @23:40:43.26 AS.reconfigure ASslow1 @23:40:43.33 AS.seek 0.01ml @23:40:45.66 delay 2 seconds @23:40:47.68 ATV.dial :bleach @23:40:48.33 AS.seek 0.81ml @23:43:29.49 delay 3 seconds @23:43:32.52 ATV.dial :air3 @23:43:33.08 delay 3 seconds @23:43:36.17 AS.seek 0.835ml @23:43:41.40 delay 3 seconds @23:43:44.43 ATV.dial :bleach @23:43:45.06 AS.seek 0.82ml @23:43:48.37 delay 3 seconds @23:43:51.40 ATV.dial PCR @23:43:51.84 AS.reconfigure ASconfig @23:43:51.90 AS.seek :empty @23:44:39.40 PCR.program :bleachProfile @23:44:39.47 PCR program should complete in < 2:45 @23:44:39.48 PCR.run 10:00 @23:47:29.22 PCR.off @23:47:29.26 AFV.select :carrierSolution @23:47:29.37 delay 2 seconds @23:47:31.41 AS.seek 1ml @23:48:29.26 delay 1 second @23:48:30.28 AFV.select :holdingCoil @23:48:30.40 delay 1 second @23:48:31.44 AS.reconfigure ASfast @23:48:31.50 AS.seek 0ml @23:48:58.86 delay 3 seconds @23:49:01.89 AFV.select :carrierSolution @23:49:02.01 delay 2 seconds @23:49:04.05 AS.reconfigure ASconfig @23:49:04.12 AS.seek 1ml @23:50:01.92 delay 1 second @23:50:02.94 AFV.select :holdingCoil @23:50:03.06 delay 1 second @23:50:04.10 AS.reconfigure ASfast @23:50:04.16 AS.seek 0ml @23:50:31.54 delay 3 seconds @23:50:34.57 AFV.select :carrierSolution @23:50:34.69 delay 2 seconds @23:50:36.73 AS.reconfigure ASconfig @23:50:36.80 AS.seek 1ml @23:51:34.57 delay 1 second @23:51:35.59 AFV.select :holdingCoil @23:51:35.71 delay 1 second @23:51:36.75 AS.reconfigure ASfast @23:51:36.81 AS.seek 0ml @23:52:04.19 delay 3 seconds @23:52:07.22 AFV.select :carrierSolution @23:52:07.34 delay 2 seconds @23:52:09.38 AS.reconfigure ASconfig @23:52:09.44 AS.seek 1ml @23:53:07.22 delay 1 second @23:53:08.24 AFV.select :holdingCoil @23:53:08.36 delay 1 second @23:53:09.40 AS.reconfigure ASfast @23:53:09.46 AS.seek 0ml @23:53:36.93 delay 3 seconds @23:53:39.96 AFV.select :carrierSolution @23:53:40.08 delay 2 seconds @23:53:42.12 AS.reconfigure ASconfig @23:53:42.19 AS.seek 1ml @23:54:40.01 delay 1 second @23:54:41.03 AFV.select :holdingCoil @23:54:41.15 delay 1 second @23:54:42.19 AS.reconfigure ASnorm @23:54:42.25 AS.seek 0ml @23:55:32.97 Setting up PCR for NM -- writing /var/log/esp/NM20jul1323h.pcr @23:55:32.99 Building PCR Slug from ASV with MM1 mix, PP1 primer @23:55:33.02 Priming with carrier @23:55:33.04 ATV.dial ARV @23:55:33.69 ARV.dial :carrier @23:55:34.24 AS.seek 0.5ml @23:55:59.91 AFV.select :carrierSolution @23:56:00.04 delay 2 seconds @23:56:02.08 AS.reconfigure ASconfig @23:56:02.15 AS.seek 1ml @23:56:31.19 delay 1 second @23:56:32.21 AFV.select :holdingCoil @23:56:32.33 delay 1 second @23:56:33.36 ATV.dial :waste @23:56:35.08 AS.reconfigure ASnorm @23:56:35.15 AS.seek :empty @23:57:25.91 AFV.select :carrierSolution @23:57:26.04 delay 2 seconds @23:57:28.08 AS.reconfigure ASconfig @23:57:28.15 AS.seek 0.5ml @23:57:57.27 delay 1 second @23:57:58.29 AFV.select :holdingCoil @23:57:58.41 Building Slug @23:57:58.43 ATV.dial :ARV @23:58:00.11 delay 2 seconds @23:58:02.14 ARV.dial :air1 @23:58:02.55 delay 2 seconds @23:58:04.64 AS.reconfigure ASslow2 @23:58:04.71 AS.seek 0.506ml @23:58:11.13 delay 2 seconds @23:58:13.15 ARV.dial :MM1 @23:58:13.77 delay 2 seconds @23:58:15.80 Adding MM1 @23:58:15.90 AS.seek 0.524ml @23:58:34.41 delay 25 seconds @23:58:59.44 ARV.dial :PP1 @23:58:59.85 Adding PP1 @23:58:59.87 delay 2 seconds @23:59:01.97 AS.seek 0.53ml @23:59:08.43 delay 10 seconds @23:59:18.46 ARV.dial :air1 @23:59:18.89 adding ARV air @23:59:18.90 delay 2 seconds @23:59:20.99 AS.seek 0.536ml @23:59:27.32 delay 5 seconds @23:59:32.35 ARV.dial :carrier @23:59:32.77 delay 7 seconds @23:59:39.86 AS.seek 0.594ml @00:00:38.33CEST14-Jul-20 delay 5 seconds @00:00:43.36 ATV.dial :ASV @00:00:43.79 delay 2 seconds @00:00:45.88 AS.seek 0.6ml @00:00:52.16 delay 4 seconds @00:00:56.19 ATV.dial ARV @00:00:56.76 delay 2 seconds @00:00:58.79 ARV.dial :carrier @00:00:58.83 delay 2 seconds @00:01:00.95 AS.seek 0.65ml @00:01:51.49 delay 4 seconds @00:01:55.52 ATV.dial PCR @00:01:56.15 ASV.dial :waste1 @00:01:56.17 ARV.dial :air1 @00:01:56.62 Positioning Slug by 0.324ml @00:01:56.72 AS.seek 0.326ml @00:07:22.68 delay 5 seconds @00:07:27.71 ATV.dial ARV @00:07:28.50 ASV.dial :PCRlock @00:07:29.50 delay 5 seconds @00:07:34.53 Running NM Profile60C300 thermocycling @00:07:34.55 PCR.program Profile60C300 @00:07:34.67 Profile60C300 should complete in < 1:59:25 @00:07:34.69 PCR.run 2:34:16.25 @01:56:19.79 PCR.off @01:56:20.48 PCR for NM completed -- closed /var/log/esp/NM20jul1323h.pcr @01:56:20.57 Cleaning PCR @01:56:20.58 AFV.select :carrierSolution @01:56:20.71 delay 2 seconds @01:56:22.75 AS.reconfigure ASconfig @01:56:22.81 AS.seek 0.5ml @01:56:33.14 delay 1 second @01:56:34.16 AFV.select :holdingCoil @01:56:34.86 ASV.dial :waste1 @01:56:35.30 ATV.dial :air3 @01:56:36.63 AS.reconfigure ASslow1 @01:56:36.70 AS.seek 0.51ml @01:56:38.98 delay 2 seconds @01:56:41.00 ATV.dial :bleach @01:56:41.66 AS.seek 0.81ml @01:57:42.25 delay 3 seconds @01:57:45.28 ATV.dial :air3 @01:57:45.84 delay 3 seconds @01:57:48.93 AS.seek 0.835ml @01:57:54.27 delay 3 seconds @01:57:57.30 ATV.dial :bleach @01:57:57.99 AS.seek 0.82ml @01:58:01.30 delay 3 seconds @01:58:04.33 ARV.dial :waste @01:58:04.97 delay 3 seconds @01:58:08.00 ATV.dial :ARV @01:58:08.93 AS.reconfigure ASconfig @01:58:09.00 AS.seek 0.47ml @01:58:29.44 AFV.select :carrierSolution @01:58:29.56 delay 2 seconds @01:58:31.60 AS.seek 1ml @01:59:02.36 delay 1 second @01:59:03.38 AFV.select :holdingCoil @01:59:03.50 delay 1 second @01:59:04.54 AS.reconfigure ASfast @01:59:04.60 AS.seek 0ml @01:59:31.97 AFV.select :carrierSolution @01:59:32.10 delay 2 seconds @01:59:34.14 AS.reconfigure ASconfig @01:59:34.20 AS.seek 1ml @02:00:32.01 delay 1 second @02:00:33.03 AFV.select :holdingCoil @02:00:33.15 delay 1 second @02:00:34.19 AS.reconfigure ASfast @02:00:34.25 AS.seek 0ml @02:01:01.61 AFV.select :carrierSolution @02:01:01.74 delay 2 seconds @02:01:03.78 AS.reconfigure ASconfig @02:01:03.84 AS.seek 1ml @02:02:01.64 delay 1 second @02:02:02.66 AFV.select :holdingCoil @02:02:02.78 delay 1 second @02:02:03.82 AS.reconfigure ASfast @02:02:03.88 AS.seek 0ml @02:02:31.25 ARV.dial between carrier and air1 @02:02:31.78 ATV.dial :air3 @02:02:33.12 AS.reconfigure ASslow1 @02:02:33.19 AS.seek 0.01ml @02:02:35.53 delay 2 seconds @02:02:37.55 ATV.dial :bleach @02:02:38.19 AS.seek 0.81ml @02:05:19.36 delay 3 seconds @02:05:22.39 ATV.dial :air3 @02:05:22.95 delay 3 seconds @02:05:26.04 AS.seek 0.835ml @02:05:31.27 delay 3 seconds @02:05:34.30 ATV.dial :bleach @02:05:34.93 AS.seek 0.82ml @02:05:38.20 delay 3 seconds @02:05:41.23 ATV.dial PCR @02:05:41.66 AS.reconfigure ASconfig @02:05:41.72 AS.seek :empty @02:06:29.19 PCR.program :bleachProfile @02:06:29.26 PCR program should complete in < 2:45 @02:06:29.27 PCR.run 10:00 @02:09:18.99 PCR.off @02:09:19.03 AFV.select :carrierSolution @02:09:19.15 delay 2 seconds @02:09:21.19 AS.seek 1ml @02:10:19.03 delay 1 second @02:10:20.05 AFV.select :holdingCoil @02:10:20.17 delay 1 second @02:10:21.21 AS.reconfigure ASfast @02:10:21.27 AS.seek 0ml @02:10:48.62 delay 3 seconds @02:10:51.65 AFV.select :carrierSolution @02:10:51.77 delay 2 seconds @02:10:53.81 AS.reconfigure ASconfig @02:10:53.88 AS.seek 1ml @02:11:51.67 delay 1 second @02:11:52.69 AFV.select :holdingCoil @02:11:52.81 delay 1 second @02:11:53.85 AS.reconfigure ASfast @02:11:53.91 AS.seek 0ml @02:12:21.28 delay 3 seconds @02:12:24.31 AFV.select :carrierSolution @02:12:24.43 delay 2 seconds @02:12:26.47 AS.reconfigure ASconfig @02:12:26.53 AS.seek 1ml @02:13:24.90 delay 1 second @02:13:25.92 AFV.select :holdingCoil @02:13:26.04 delay 1 second @02:13:27.08 AS.reconfigure ASfast @02:13:27.14 AS.seek 0ml @02:13:54.50 delay 3 seconds @02:13:57.53 AFV.select :carrierSolution @02:13:57.65 delay 2 seconds @02:13:59.69 AS.reconfigure ASconfig @02:13:59.76 AS.seek 1ml @02:14:57.58 delay 1 second @02:14:58.60 AFV.select :holdingCoil @02:14:58.72 delay 1 second @02:14:59.76 AS.reconfigure ASfast @02:14:59.82 AS.seek 0ml @02:15:27.20 delay 3 seconds @02:15:30.23 AFV.select :carrierSolution @02:15:30.35 delay 2 seconds @02:15:32.39 AS.reconfigure ASconfig @02:15:32.45 AS.seek 1ml @02:16:30.23 delay 1 second @02:16:31.25 AFV.select :holdingCoil @02:16:31.37 delay 1 second @02:16:32.41 AS.reconfigure ASnorm @02:16:32.47 AS.seek 0ml @02:17:23.22 Setting up PCR for IPc -- writing /var/log/esp/IPc20jul1402h.pcr @02:17:23.24 Building PCR Slug from ASV with MM3 mix, PP6 primer @02:17:23.27 Priming with carrier @02:17:23.29 ATV.dial ARV @02:17:24.06 ARV.dial :carrier @02:17:24.44 AS.seek 0.5ml @02:17:50.03 AFV.select :carrierSolution @02:17:50.16 delay 2 seconds @02:17:52.20 AS.reconfigure ASconfig @02:17:52.27 AS.seek 1ml @02:18:21.31 delay 1 second @02:18:22.33 AFV.select :holdingCoil @02:18:22.45 delay 1 second @02:18:23.48 ATV.dial :waste @02:18:25.19 AS.reconfigure ASnorm @02:18:25.26 AS.seek :empty @02:19:16.01 AFV.select :carrierSolution @02:19:16.14 delay 2 seconds @02:19:18.18 AS.reconfigure ASconfig @02:19:18.24 AS.seek 0.5ml @02:19:47.39 delay 1 second @02:19:48.41 AFV.select :holdingCoil @02:19:48.55 Building Slug @02:19:48.57 ATV.dial :ARV @02:19:50.26 delay 2 seconds @02:19:52.29 ARV.dial :air1 @02:19:52.71 delay 2 seconds @02:19:54.80 AS.reconfigure ASslow2 @02:19:54.87 AS.seek 0.506ml @02:20:01.15 delay 2 seconds @02:20:03.18 ARV.dial :MM3 @02:20:04.26 delay 2 seconds @02:20:06.29 Adding MM3 @02:20:06.40 AS.seek 0.524ml @02:20:24.64 delay 25 seconds @02:20:49.67 ARV.dial :PP6 @02:20:50.08 Adding PP6 @02:20:50.10 delay 2 seconds @02:20:52.19 AS.seek 0.53ml @02:20:58.43 delay 10 seconds @02:21:08.46 ARV.dial :air1 @02:21:09.31 adding ARV air @02:21:09.33 delay 2 seconds @02:21:11.43 AS.seek 0.536ml @02:21:17.59 delay 5 seconds @02:21:22.62 ARV.dial :carrier @02:21:23.06 delay 7 seconds @02:21:30.15 AS.seek 0.594ml @02:22:28.68 delay 5 seconds @02:22:33.71 ATV.dial :ASV @02:22:34.14 delay 2 seconds @02:22:36.23 AS.seek 0.6ml @02:22:42.54 delay 4 seconds @02:22:46.57 ATV.dial ARV @02:22:47.14 delay 2 seconds @02:22:49.17 ARV.dial :carrier @02:22:49.19 delay 2 seconds @02:22:51.28 AS.seek 0.65ml @02:23:41.93 delay 4 seconds @02:23:45.96 ATV.dial PCR @02:23:46.59 ASV.dial :waste1 @02:23:46.61 ARV.dial :air1 @02:23:47.05 Positioning Slug by 0.324ml @02:23:47.17 AS.seek 0.326ml @02:29:13.23 delay 5 seconds @02:29:18.26 ATV.dial ARV @02:29:19.06 ASV.dial :PCRlock @02:29:19.48 delay 5 seconds @02:29:24.51 Running IPc ProfileIPc thermocycling @02:29:24.53 PCR.program ProfileIPc @02:29:24.66 ProfileIPc should complete in < 1:36:15 @02:29:24.68 PCR.run 2:05:18.75 @04:01:38.71 PCR.off @04:01:39.28 PCR for IPc completed -- closed /var/log/esp/IPc20jul1402h.pcr @04:01:39.37 Cleaning PCR @04:01:39.39 AFV.select :carrierSolution @04:01:39.52 delay 2 seconds @04:01:41.56 AS.reconfigure ASconfig @04:01:41.63 AS.seek 0.5ml @04:01:51.96 delay 1 second @04:01:52.98 AFV.select :holdingCoil @04:01:53.10 ASV.dial :waste1 @04:01:53.51 ATV.dial :air3 @04:01:54.86 AS.reconfigure ASslow1 @04:01:54.93 AS.seek 0.51ml @04:01:57.22 delay 2 seconds @04:01:59.24 ATV.dial :bleach @04:01:59.89 AS.seek 0.81ml @04:03:00.51 delay 3 seconds @04:03:03.54 ATV.dial :air3 @04:03:04.12 delay 3 seconds @04:03:07.21 AS.seek 0.835ml @04:03:12.51 delay 3 seconds @04:03:15.54 ATV.dial :bleach @04:03:16.19 AS.seek 0.82ml @04:03:19.52 delay 3 seconds @04:03:22.55 ARV.dial :waste @04:03:23.16 delay 3 seconds @04:03:26.19 ATV.dial :ARV @04:03:27.11 AS.reconfigure ASconfig @04:03:27.18 AS.seek 0.47ml @04:03:47.62 AFV.select :carrierSolution @04:03:47.75 delay 2 seconds @04:03:49.79 AS.seek 1ml @04:04:20.58 delay 1 second @04:04:21.60 AFV.select :holdingCoil @04:04:21.72 delay 1 second @04:04:22.76 AS.reconfigure ASfast @04:04:22.82 AS.seek 0ml @04:04:50.19 AFV.select :carrierSolution @04:04:50.32 delay 2 seconds @04:04:52.36 AS.reconfigure ASconfig @04:04:52.42 AS.seek 1ml @04:05:50.18 delay 1 second @04:05:51.20 AFV.select :holdingCoil @04:05:51.32 delay 1 second @04:05:52.36 AS.reconfigure ASfast @04:05:52.42 AS.seek 0ml @04:06:19.77 AFV.select :carrierSolution @04:06:19.90 delay 2 seconds @04:06:21.94 AS.reconfigure ASconfig @04:06:22.00 AS.seek 1ml @04:07:19.80 delay 1 second @04:07:20.82 AFV.select :holdingCoil @04:07:20.94 delay 1 second @04:07:21.98 AS.reconfigure ASfast @04:07:22.04 AS.seek 0ml @04:07:49.41 ARV.dial between carrier and air1 @04:07:49.93 ATV.dial :air3 @04:07:51.26 AS.reconfigure ASslow1 @04:07:51.33 AS.seek 0.01ml @04:07:53.68 delay 2 seconds @04:07:55.70 ATV.dial :bleach @04:07:56.36 AS.seek 0.81ml @04:10:37.52 delay 3 seconds @04:10:40.55 ATV.dial :air3 @04:10:41.12 delay 3 seconds @04:10:44.21 AS.seek 0.835ml @04:10:49.44 delay 3 seconds @04:10:52.47 ATV.dial :bleach @04:10:53.09 AS.seek 0.82ml @04:10:56.38 delay 3 seconds @04:10:59.41 ATV.dial PCR @04:10:59.83 AS.reconfigure ASconfig @04:10:59.90 AS.seek :empty @04:11:47.40 PCR.program :bleachProfile @04:11:47.47 PCR program should complete in < 2:45 @04:11:47.48 PCR.run 10:00 @04:14:37.76 PCR.off @04:14:37.80 AFV.select :carrierSolution @04:14:37.93 delay 2 seconds @04:14:39.97 AS.seek 1ml @04:15:37.82 delay 1 second @04:15:38.84 AFV.select :holdingCoil @04:15:38.96 delay 1 second @04:15:40.00 AS.reconfigure ASfast @04:15:40.06 AS.seek 0ml @04:16:07.44 delay 3 seconds @04:16:10.47 AFV.select :carrierSolution @04:16:10.59 delay 2 seconds @04:16:12.63 AS.reconfigure ASconfig @04:16:12.73 AS.seek 1ml @04:17:10.54 delay 1 second @04:17:11.56 AFV.select :holdingCoil @04:17:11.68 delay 1 second @04:17:12.72 AS.reconfigure ASfast @04:17:12.78 AS.seek 0ml @04:17:40.15 delay 3 seconds @04:17:43.18 AFV.select :carrierSolution @04:17:43.30 delay 2 seconds @04:17:45.34 AS.reconfigure ASconfig @04:17:45.40 AS.seek 1ml @04:18:43.18 delay 1 second @04:18:44.20 AFV.select :holdingCoil @04:18:44.32 delay 1 second @04:18:45.36 AS.reconfigure ASfast @04:18:45.42 AS.seek 0ml @04:19:12.79 delay 3 seconds @04:19:15.82 AFV.select :carrierSolution @04:19:15.94 delay 2 seconds @04:19:17.98 AS.reconfigure ASconfig @04:19:18.05 AS.seek 1ml @04:20:15.83 delay 1 second @04:20:16.85 AFV.select :holdingCoil @04:20:16.97 delay 1 second @04:20:18.01 AS.reconfigure ASfast @04:20:18.07 AS.seek 0ml @04:20:45.46 delay 3 seconds @04:20:48.49 AFV.select :carrierSolution @04:20:48.61 delay 2 seconds @04:20:50.65 AS.reconfigure ASconfig @04:20:50.72 AS.seek 1ml @04:21:48.51 delay 1 second @04:21:49.53 AFV.select :holdingCoil @04:21:49.65 delay 1 second @04:21:50.69 AS.reconfigure ASnorm @04:21:50.75 AS.seek 0ml @04:22:41.46 Setting up PCR for IPc2 -- writing /var/log/esp/IPc220jul1404h.pcr @04:22:41.49 Building PCR Slug from ASV with MM2 mix, PP3 primer @04:22:41.52 Priming with carrier @04:22:41.54 ATV.dial ARV @04:22:42.18 ARV.dial :carrier @04:22:42.54 AS.seek 0.5ml @04:23:08.16 AFV.select :carrierSolution @04:23:08.29 delay 2 seconds @04:23:10.33 AS.reconfigure ASconfig @04:23:10.40 AS.seek 1ml @04:23:39.44 delay 1 second @04:23:40.46 AFV.select :holdingCoil @04:23:40.58 delay 1 second @04:23:41.61 ATV.dial :waste @04:23:43.33 AS.reconfigure ASnorm @04:23:43.40 AS.seek :empty @04:24:34.16 AFV.select :carrierSolution @04:24:34.29 delay 2 seconds @04:24:36.33 AS.reconfigure ASconfig @04:24:36.39 AS.seek 0.5ml @04:25:05.52 delay 1 second @04:25:06.54 AFV.select :holdingCoil @04:25:06.66 Building Slug @04:25:06.68 ATV.dial :ARV @04:25:08.37 delay 2 seconds @04:25:10.40 ARV.dial :air1 @04:25:10.81 delay 2 seconds @04:25:12.90 AS.reconfigure ASslow2 @04:25:12.97 AS.seek 0.506ml @04:25:19.44 delay 2 seconds @04:25:21.46 ARV.dial :MM2 @04:25:22.99 delay 2 seconds @04:25:25.02 Adding MM2 @04:25:25.13 AS.seek 0.524ml @04:25:43.44 delay 25 seconds @04:26:08.47 ARV.dial :PP3 @04:26:08.88 Adding PP3 @04:26:08.90 delay 2 seconds @04:26:11.00 AS.seek 0.53ml @04:26:17.30 delay 10 seconds @04:26:27.33 ARV.dial :air2 @04:26:27.74 adding ARV air @04:26:27.76 delay 2 seconds @04:26:29.85 AS.seek 0.536ml @04:26:36.26 delay 5 seconds @04:26:41.29 ARV.dial :carrier @04:26:42.60 delay 7 seconds @04:26:49.69 AS.seek 0.594ml @04:27:48.27 delay 5 seconds @04:27:53.30 ATV.dial :ASV @04:27:53.72 delay 2 seconds @04:27:55.81 AS.seek 0.6ml @04:28:02.02 delay 4 seconds @04:28:06.05 ATV.dial ARV @04:28:06.62 delay 2 seconds @04:28:08.65 ARV.dial :carrier @04:28:08.67 delay 2 seconds @04:28:10.76 AS.seek 0.65ml @04:29:01.24 delay 4 seconds @04:29:05.83 ATV.dial PCR @04:29:06.47 ASV.dial :waste1 @04:29:06.49 ARV.dial :air1 @04:29:06.93 Positioning Slug by 0.324ml @04:29:07.05 AS.seek 0.326ml @04:34:33.08 delay 5 seconds @04:34:38.11 ATV.dial ARV @04:34:38.91 ASV.dial :PCRlock @04:34:39.33 delay 5 seconds @04:34:44.36 Running IPc2 ProfileIPc thermocycling @04:34:44.38 PCR.program ProfileIPc @04:34:44.50 ProfileIPc should complete in < 1:36:15 @04:34:44.52 PCR.run 2:05:18.75 @06:06:29.31 PCR.off @06:06:29.89 PCR for IPc2 completed -- closed /var/log/esp/IPc220jul1404h.pcr @06:06:29.91 Full MFB Decontamination @06:06:29.93 Decon extraction column and decon AIV @06:06:29.97 SPE.hold 2:46:40,55C @06:06:30.02 ASV.dial :SPEdelivery @06:06:30.70 delay 20 seconds @06:06:50.73 AFV.select :carrierSolution @06:06:50.85 delay 2 seconds @06:06:52.89 AS.reconfigure ASconfig @06:06:52.95 AS.seek 0.5ml @06:07:03.27 delay 1 second @06:07:04.29 AFV.select :holdingCoil @06:07:04.41 ATV.dial :air1 @06:07:06.49 AS.reconfigure ASslow1 @06:07:06.56 AS.seek 0.52ml @06:07:10.92 ATV.dial :ASV @06:07:12.86 ASV.dial :SPErecovery @06:07:13.59 AS.seek 0.62ml @06:07:34.06 ATV.dial :waste @06:07:36.26 AS.reconfigure ASnorm @06:07:36.33 AS.seek :empty @06:08:08.00 AFV.select :holdingCoil @06:08:08.01 ATV.dial :waste @06:08:08.05 AS.reconfigure ASconfig @06:08:08.13 AS.seek 0ml @06:08:08.50 AFV.select :carrierSolution @06:08:08.63 delay 2 seconds @06:08:10.67 AS.seek 0.5ml @06:08:39.69 delay 1 second @06:08:40.71 AFV.select :holdingCoil @06:08:40.83 ATV.dial :air3 @06:08:41.62 AS.reconfigure ASslow1 @06:08:41.69 AS.seek 0.52ml @06:08:46.00 ATV.dial :air3 @06:08:46.11 AS.seek 0.53ml @06:08:48.41 delay 2 seconds @06:08:50.43 ATV.dial :bleach @06:08:50.96 AS.seek 0.71ml @06:09:27.47 delay 3 seconds @06:09:30.50 ATV.dial :air3 @06:09:31.11 delay 3 seconds @06:09:34.20 AS.seek 0.735ml @06:09:39.52 delay 3 seconds @06:09:42.55 ATV.dial :bleach @06:09:43.23 AS.seek 0.72ml @06:09:46.59 delay 3 seconds @06:09:49.62 ATV.dial ASV @06:09:50.78 ASV.dial :SPErecovery @06:09:50.88 AS.seek 0.365ml @06:11:02.55 ATV.dial :waste @06:11:04.40 AS.reconfigure ASnorm @06:11:04.47 AS.seek :empty @06:11:23.27 ATV.dial :air3 @06:11:24.01 AS.reconfigure ASslow1 @06:11:24.08 AS.seek 0.009ml @06:11:26.45 delay 2 seconds @06:11:28.47 ATV.dial :bleach @06:11:28.96 AS.seek 0.579ml @06:13:23.86 delay 3 seconds @06:13:26.89 ATV.dial :air3 @06:13:27.47 delay 3 seconds @06:13:31.14 AS.seek 0.604ml @06:13:36.45 delay 3 seconds @06:13:39.48 ATV.dial :bleach @06:13:40.14 AS.seek 0.589ml @06:13:43.45 delay 3 seconds @06:13:46.48 ATV.dial :ASV @06:13:47.56 ASV.dial :wasteR @06:13:47.98 AS.seek 0ml @06:15:46.75 delay 1:00 @06:16:46.78 ATV.dial ASV @06:16:46.79 AFV.select :carrierSolution @06:16:46.92 delay 2 seconds @06:16:48.96 AS.reconfigure ASconfig @06:16:49.02 AS.seek 1ml @06:17:46.80 delay 1 second @06:17:47.82 AFV.select :holdingCoil @06:17:47.94 delay 1 second @06:17:48.97 ASV.dial :wasteR @06:17:48.99 AS.reconfigure ASslow1 @06:17:49.07 AS.seek 0ml @06:21:10.48 delay 10 seconds @06:21:20.51 ATV.dial :air3 @06:21:22.08 AS.seek 0.01ml @06:21:24.41 delay 2 seconds @06:21:26.43 ATV.dial :bleach @06:21:27.09 AS.seek 0.58ml @06:23:22.01 delay 3 seconds @06:23:25.04 ATV.dial :air3 @06:23:25.62 delay 3 seconds @06:23:28.71 AS.seek 0.605ml @06:23:34.01 delay 3 seconds @06:23:37.04 ATV.dial :bleach @06:23:37.69 AS.seek 0.59ml @06:23:41.01 delay 3 seconds @06:23:44.04 ATV.dial ASV @06:23:45.12 ASV.dial :SPEdelivery @06:23:45.60 AS.seek 0ml @06:25:44.53 delay 1:00 @06:26:44.56 AFV.select :carrierSolution @06:26:44.68 delay 2 seconds @06:26:46.72 AS.reconfigure ASconfig @06:26:46.78 AS.seek 1ml @06:27:44.56 delay 1 second @06:27:45.59 AFV.select :holdingCoil @06:27:45.72 delay 1 second @06:27:46.75 ATV.dial ASV @06:27:46.76 ASV.dial :SPEdelivery @06:27:46.80 AS.reconfigure ASslow1 @06:27:46.87 AS.seek 0ml @06:31:08.30 delay 10 seconds @06:31:18.33 ATV.dial :air2 @06:31:19.21 AS.reconfigure ASslow0 @06:31:19.28 AS.seek 0.8ml @06:32:40.08 delay 3 seconds @06:32:43.11 ATV.dial :ASV @06:32:44.12 ASV.dial :SPEdelivery @06:32:44.15 AS.reconfigure ASslow1 @06:32:44.22 AS.seek 0.03ml @06:35:19.33 delay 20 seconds @06:35:39.36 ATV.dial :waste,via: :air3 @06:35:41.17 AS.reconfigure ASconfig @06:35:41.23 AS.seek :empty @06:35:43.31 SPE.coast @06:35:43.41 Removing Bleach from Mixing Coil @06:35:43.43 AFV.select :carrierSolution @06:35:43.55 delay 2 seconds @06:35:45.59 AS.seek 0.5ml @06:36:14.70 delay 1 second @06:36:15.72 AFV.select :holdingCoil @06:36:15.84 ATV.dial :ASV @06:36:17.58 ASV.dial :SPErecovery @06:36:18.26 AS.reconfigure ASslow1 @06:36:18.33 AS.seek 0.73ml @06:37:04.84 ASV.dial :wasteD @06:37:05.45 AS.reconfigure ASnorm @06:37:05.52 AS.seek 0ml @06:37:42.65 AFV.select :carrierSolution @06:37:42.78 delay 2 seconds @06:37:44.82 AS.reconfigure ASconfig @06:37:44.88 AS.seek 0.5ml @06:38:13.92 delay 1 second @06:38:14.94 AFV.select :holdingCoil @06:38:15.06 ATV.dial ASV @06:38:15.08 ASV.dial :SPErecovery @06:38:15.76 AS.reconfigure ASslow0 @06:38:15.83 AS.seek 0.3ml @06:38:36.26 ATV.dial :air2 @06:38:37.33 AS.reconfigure ASslow1 @06:38:37.40 AS.seek 0.32ml @06:38:41.78 ATV.dial ASV @06:38:42.87 AS.reconfigure ASslow0 @06:38:42.94 AS.seek 0.57ml @06:39:08.50 ASV.dial :wasteD @06:39:09.70 AS.reconfigure ASnorm @06:39:09.77 AS.seek 0ml @06:39:38.87 AFV.select :carrierSolution @06:39:39.00 delay 2 seconds @06:39:41.04 AS.reconfigure ASconfig @06:39:41.10 AS.seek 0.5ml @06:40:10.17 delay 1 second @06:40:11.19 AFV.select :holdingCoil @06:40:11.31 ATV.dial ASV @06:40:11.33 ASV.dial :SPErecovery @06:40:12.01 AS.reconfigure ASslow0 @06:40:12.09 AS.seek 0.3ml @06:40:32.50 ATV.dial :air2 @06:40:33.58 AS.reconfigure ASslow1 @06:40:33.66 AS.seek 0.32ml @06:40:38.04 ATV.dial ASV @06:40:39.13 AS.reconfigure ASslow0 @06:40:39.21 AS.seek 0.57ml @06:41:04.65 ASV.dial :wasteD @06:41:05.26 AS.reconfigure ASnorm @06:41:05.33 AS.seek 0ml @06:41:34.42 ATV.dial :air2 @06:41:35.45 AS.reconfigure ASslow0 @06:41:35.52 AS.seek 0.3ml @06:42:06.05 ATV.dial :ASV @06:42:07.06 ASV.dial :wasteD @06:42:07.09 AS.reconfigure ASconfig @06:42:07.16 AS.seek 0.05ml @06:42:21.87 ATV.dial :waste @06:42:23.79 AS.seek 0ml @06:42:27.01 ASV.dial :SPEdelivery @06:42:28.11 deconLysateINT @06:42:28.13 ATV.dial :air3 @06:42:28.85 AS.reconfigure ASslow1 @06:42:28.92 AS.seek 0.01ml @06:42:31.33 delay 2 seconds @06:42:33.35 ATV.dial :bleach @06:42:33.85 AS.seek 0.96ml @06:45:45.20 delay 3 seconds @06:45:48.23 ATV.dial :air3 @06:45:48.81 delay 3 seconds @06:45:51.90 AS.seek 0.985ml @06:45:57.21 delay 3 seconds @06:46:00.24 ATV.dial :bleach @06:46:00.88 AS.seek 0.97ml @06:46:04.20 delay 3 seconds @06:46:07.23 ATV.dial AIV @06:46:08.51 AIV.dial :coilToMFB @06:46:08.53 AWV.select :waste @06:46:08.56 AFV.select :carrierSolution @06:46:08.69 delay 2 seconds @06:46:10.73 AS.reconfigure ASconfig @06:46:10.80 AS.seek 1ml @06:46:12.84 delay 1 second @06:46:13.86 AFV.select :holdingCoil @06:46:13.98 delay 1 second @06:46:15.02 AS.reconfigure ASslow0 @06:46:15.08 AS.seek :empty @06:47:56.04 AFV.select :carrierSolution @06:47:56.17 delay 2 seconds @06:47:58.21 AS.reconfigure ASconfig @06:47:58.28 AS.seek 1ml @06:48:56.12 delay 1 second @06:48:57.14 AFV.select :holdingCoil @06:48:57.26 delay 1 second @06:48:58.30 AS.reconfigure ASslow0 @06:48:58.36 AS.seek :empty @06:50:39.37 AFV.select :carrierSolution @06:50:39.50 delay 2 seconds @06:50:41.54 AS.reconfigure ASconfig @06:50:41.60 AS.seek 1ml @06:51:39.45 delay 1 second @06:51:40.47 AFV.select :holdingCoil @06:51:40.59 delay 1 second @06:51:41.63 AS.reconfigure ASslow0 @06:51:41.69 AS.seek :empty @06:53:22.67 ATV.dial :air2 @06:53:23.38 AS.reconfigure ASslow1 @06:53:23.45 AS.seek 0.009ml @06:53:25.79 AS.reconfigure ASslow0 @06:53:25.87 AS.seek 1ml @06:55:05.81 ATV.dial AIV @06:55:06.63 AS.seek 0.02ml @06:56:45.53 ATV.dial :waste @06:56:47.24 AS.seek 0ml @06:56:49.54 AWV.select :air @06:56:49.73 Cleaning PCR @06:56:49.74 AFV.select :carrierSolution @06:56:49.87 delay 2 seconds @06:56:51.91 AS.reconfigure ASconfig @06:56:51.97 AS.seek 0.5ml @06:57:21.62 delay 1 second @06:57:22.64 AFV.select :holdingCoil @06:57:22.76 ASV.dial :waste1 @06:57:23.15 ATV.dial :air3 @06:57:23.88 AS.reconfigure ASslow1 @06:57:23.95 AS.seek 0.51ml @06:57:26.23 delay 2 seconds @06:57:28.25 ATV.dial :bleach @06:57:28.73 AS.seek 0.81ml @06:58:29.33 delay 3 seconds @06:58:32.36 ATV.dial :air3 @06:58:32.94 delay 3 seconds @06:58:36.03 AS.seek 0.835ml @06:58:41.35 delay 3 seconds @06:58:44.38 ATV.dial :bleach @06:58:45.02 AS.seek 0.82ml @06:58:48.36 delay 3 seconds @06:58:51.39 ARV.dial :waste @06:58:52.03 delay 3 seconds @06:58:55.06 ATV.dial :ARV @06:58:55.97 AS.reconfigure ASconfig @06:58:56.04 AS.seek 0.47ml @06:59:16.47 AFV.select :carrierSolution @06:59:16.60 delay 2 seconds @06:59:18.64 AS.seek 1ml @06:59:49.43 delay 1 second @06:59:50.45 AFV.select :holdingCoil @06:59:50.57 delay 1 second @06:59:51.61 AS.reconfigure ASfast @06:59:51.67 AS.seek 0ml @07:00:19.03 AFV.select :carrierSolution @07:00:19.16 delay 2 seconds @07:00:21.20 AS.reconfigure ASconfig @07:00:21.26 AS.seek 1ml @07:01:19.04 delay 1 second @07:01:20.06 AFV.select :holdingCoil @07:01:20.18 delay 1 second @07:01:21.22 AS.reconfigure ASfast @07:01:21.28 AS.seek 0ml @07:01:48.64 AFV.select :carrierSolution @07:01:48.76 delay 2 seconds @07:01:50.80 AS.reconfigure ASconfig @07:01:50.86 AS.seek 1ml @07:02:48.67 delay 1 second @07:02:49.69 AFV.select :holdingCoil @07:02:49.81 delay 1 second @07:02:50.85 AS.reconfigure ASfast @07:02:50.91 AS.seek 0ml @07:03:18.28 ARV.dial between carrier and air1 @07:03:18.81 ATV.dial :air3 @07:03:20.15 AS.reconfigure ASslow1 @07:03:20.22 AS.seek 0.01ml @07:03:22.61 delay 2 seconds @07:03:24.63 ATV.dial :bleach @07:03:25.29 AS.seek 0.81ml @07:06:06.43 delay 3 seconds @07:06:09.46 ATV.dial :air3 @07:06:10.03 delay 3 seconds @07:06:13.12 AS.seek 0.835ml @07:06:18.36 delay 3 seconds @07:06:21.39 ATV.dial :bleach @07:06:22.02 AS.seek 0.82ml @07:06:25.33 delay 3 seconds @07:06:28.36 ATV.dial PCR @07:06:28.80 AS.reconfigure ASconfig @07:06:28.86 AS.seek :empty @07:07:16.36 PCR.program :bleachProfile @07:07:16.43 PCR program should complete in < 2:45 @07:07:16.45 PCR.run 10:00 @07:10:06.16 PCR.off @07:10:06.20 AFV.select :carrierSolution @07:10:06.32 delay 2 seconds @07:10:08.36 AS.seek 1ml @07:11:06.22 delay 1 second @07:11:07.24 AFV.select :holdingCoil @07:11:07.36 delay 1 second @07:11:08.40 AS.reconfigure ASfast @07:11:08.46 AS.seek 0ml @07:11:35.81 delay 3 seconds @07:11:38.84 AFV.select :carrierSolution @07:11:38.96 delay 2 seconds @07:11:41.00 AS.reconfigure ASconfig @07:11:41.07 AS.seek 1ml @07:12:38.87 delay 1 second @07:12:39.89 AFV.select :holdingCoil @07:12:40.01 delay 1 second @07:12:41.05 AS.reconfigure ASfast @07:12:41.11 AS.seek 0ml @07:13:09.09 delay 3 seconds @07:13:12.12 AFV.select :carrierSolution @07:13:12.24 delay 2 seconds @07:13:14.28 AS.reconfigure ASconfig @07:13:14.35 AS.seek 1ml @07:14:12.17 delay 1 second @07:14:13.19 AFV.select :holdingCoil @07:14:13.31 delay 1 second @07:14:14.35 AS.reconfigure ASfast @07:14:14.41 AS.seek 0ml @07:14:41.79 delay 3 seconds @07:14:44.81 AFV.select :carrierSolution @07:14:44.91 delay 2 seconds @07:14:46.95 AS.reconfigure ASconfig @07:14:47.08 AS.seek 1ml @07:15:44.89 delay 1 second @07:15:45.91 AFV.select :holdingCoil @07:15:46.03 delay 1 second @07:15:47.07 AS.reconfigure ASfast @07:15:47.13 AS.seek 0ml @07:16:14.51 delay 3 seconds @07:16:17.54 AFV.select :carrierSolution @07:16:17.66 delay 2 seconds @07:16:19.70 AS.reconfigure ASconfig @07:16:19.77 AS.seek 1ml @07:17:17.56 delay 1 second @07:17:18.58 AFV.select :holdingCoil @07:17:18.70 delay 1 second @07:17:19.74 AS.reconfigure ASnorm @07:17:19.80 AS.seek 0ml @07:18:10.55 AFV.select :carrierSolution @07:18:10.67 delay 2 seconds @07:18:12.71 AS.reconfigure ASconfig @07:18:12.77 AS.seek 1ml @07:19:10.57 delay 1 second @07:19:11.59 AFV.select :holdingCoil @07:19:11.71 delay 1 second @07:19:12.74 ATV.dial :waste @07:19:14.01 AS.reconfigure ASnorm @07:19:14.08 AS.seek 0.5ml @07:19:39.65 ATV.dial :waste2 @07:19:40.98 AS.seek 0ml @07:20:06.53
Asleep after completing InvasivePostCoreNeg assays @07:20:06.55 Mission runtime was 16:18:26.849071 @07:20:06.58 GoodNews.email "{1 => 3, 2 => 0}\nCan@07:11:41, 23.3C, 50% humidity, 14.6psia, 12.016V, 0.607A, 0.607A avg, 7.29W",Subject: "Asleep after completing InvasivePostCoreNeg assays" @07:20:15.26 Session Ended @13:38:28.89CEST14-Jul-20
ESP2 v3.30 I2C Gateway v3.06 for core bus @13:38:30.82 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @13:38:31.12 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @13:38:36.52 Polling Can every 10:00 @13:38:38.34 SPE.coast @13:38:38.40 SPE.configure SPEconfig @13:38:38.49 AS.configure ASconfig @13:38:38.59 SS.reconfigure SSconfig @13:38:38.63 SS.hold @13:38:38.69 SS.configure SSconfig @13:38:38.79 CC.reconfigure CCconfig @13:38:38.84 CC.hold @13:38:38.90 CC.configure CCconfig @13:38:39.01 CS.reconfigure CSconfig @13:38:39.05 CS.hold @13:38:39.12 CS.configure CSconfig @13:38:39.22 PC.reconfigure PCconfig @13:38:39.27 PC.hold @13:38:39.70 PC.configure PCconfig @13:38:39.79 PS.configure PSconfig @13:38:39.91 Forearm.reconfigure LooseForearm @13:38:39.95 Forearm.hold @13:38:40.00 Forearm.configure LooseForearm @13:38:40.10 Elbow.reconfigure LooseElbow @13:38:40.14 Elbow.hold @13:38:40.19 Elbow.configure LooseElbow @13:38:40.31 SC.reconfigure SCloose @13:38:40.35 SC.hold @13:38:40.40 SC.configure SCloose @13:38:40.50 SE.reconfigure SEconfig @13:38:40.54 SE.hold @13:38:40.59 SE.configure SEconfig @13:38:40.64 CH.coast @13:38:40.69 CH.configure CHconfig @13:38:40.72 PH.coast @13:38:40.78 PH.configure PHconfig @13:38:40.81 PCR.off @13:38:40.85 PCR.pidtemp= 50,0.1,0,300,25,25 @13:38:40.88 PCR.caltemp= 2.8814,0.0372,0 @13:38:40.91 All dwarf controllers running firmware version 3.75 @13:38:41.05 Loading /home/esp/esp2/mission/InvasivePostCoreNeg.rb mission @13:38:41.12 Preparing to sleep until +00:01:00 @13:38:41.14 GoodNews.email "Starting InvasivePostCoreNeg w/pucks in tube 2\n{}\nCan@13:38:36, 23.6C, 52% humidity, 14.6psia, 11.952V, 0.834A, 0.821A avg, 9.97W",Subject: "InvasivePostCoreNeg begun" @13:38:41.24 SS.coast @13:38:41.30
Asleep til +00:01:00 @13:38:41.31 CC.coast @13:38:41.34
Continuing at 13:39 @13:38:41.42 CS.coast @13:38:41.49 PC.coast @13:38:41.52 Forearm.coast @13:38:41.56 Elbow.coast @13:38:41.60 SC.coast @13:38:41.66 SE.coast @13:38:42.10 Gate.power :core,:OFF @13:38:42.12 Gate.power :raw,:OFF @13:38:42.14 Shutdown @13:38:42.60 Session Begun @13:38:48.85 GoodNews.email "Then, in tube 2: coreNEG\n/home/esp/esp2/mission/InvasivePostCoreNeg.rb:113\n{}\nCan@13:38:41, 23.5C, 52% humidity, 14.6psia, 11.962V, 0.809A, 0.809A avg, 9.67W",Subject: "Asleep until 13:39" @13:39:41.38
Powering core bus @13:39:46.61 Gate.power :raw,:ON @13:39:49.85 SPE.coast @13:39:50.12 SPE.configure SPEconfig @13:39:50.54 AS.configure ASconfig @13:39:50.98 SS.configure SSconfig @13:39:51.37 CC.configure CCconfig @13:39:51.79 CS.configure CSconfig @13:39:52.18 PC.configure PCconfig @13:39:52.61 PS.configure PSconfig @13:39:53.11 Forearm.configure LooseForearm @13:39:53.48 Elbow.configure LooseElbow @13:39:53.91 SC.configure SCloose @13:39:54.29 SE.configure SEconfig @13:39:54.50 CH.coast @13:39:54.73 CH.configure CHconfig @13:39:54.89 PH.coast @13:39:55.13 PH.configure PHconfig @13:39:55.33 PCR.off @13:39:55.45 PCR.pidtemp= 50,0.1,0,300,25,25 @13:39:55.63 PCR.caltemp= 2.8814,0.0372,0 @13:39:55.77 All dwarf controllers running firmware version 3.75 @13:39:56.09 Valve.reset @13:39:56.56 PCR.off @13:39:56.68 PCR.pidtemp= 50,0.1,0,300,25,25 @13:39:56.84 PCR.caltemp= 2.8814,0.0372,0 @13:39:57.35 Intake.select :close @13:39:57.62 Exhaust.select :close @13:39:57.69 CTV.dial :air @13:39:58.03 PSV.dial :air @13:39:58.20 CSV.dial :waste1 @13:39:58.88 SSV.dial :spit,avoiding: :exhaust @13:39:59.02 PS.reconfigure PSconfig @13:39:59.08
Forearm.reconfigure LooseForearm @13:39:59.67 PS.home.seek 0ml @13:39:59.78
Forearm.home.seek :retract @13:40:01.76 Hand.close @13:40:01.97
Elbow.jog 1500 @13:40:02.36 CS.reconfigure CSfast @13:40:02.44 SS.reconfigure SSconfig @13:40:02.82 CS.home.seek 0ml @13:40:02.94 SS.home.seek 0ml @13:40:03.31
Elbow.reconfigure LooseElbow @13:40:03.53 Elbow.home.seek :clear @13:40:08.58 SE.reconfigure SEconfig @13:40:08.79 SE.home.seek :down @13:40:09.15 PS.coast @13:40:09.63
SC.reconfigure SCsloppy @13:40:09.85 SC.home.seek :initial @13:40:20.32 Forearm.seek :retract @13:40:20.69 Elbow.seek :hammer @13:40:22.05 Forearm.reconfigure TightForearm @13:40:22.26 Forearm.seek 5 + 350 counts @13:40:23.65 Forearm.seek :retract @13:40:25.52 PC.reconfigure PCconfig @13:40:25.58
CC.reconfigure CCconfig @13:40:25.93 CC.home.seek :open @13:40:26.05 PC.home.seek :open @13:40:30.57
Phase [coreNEG] @13:40:30.60 GoodNews.email "At 13:40:30 began in tube 2: coreNEG\n/home/esp/esp2/mission/InvasivePostCoreNeg.rb:113\n{}\nCan@13:40:30, 23.5C, 52% humidity, 14.6psia, 11.962V, 0.809A, 0.809A avg, 9.67W",Subject: "Started InvasivePostCoreNeg phase coreNEG" @13:40:30.79CEST14-Jul-20
==================> STARTED coreNEG (phase #1) <=== @13:40:31.27 Starting mfb @13:40:31.36 pcrBAC wcr sampling at most 100ml @13:40:31.89 Loading sh1 puck from tube 2 into Collection Clamp @13:40:32.47 Readying the MFB @13:40:32.54
Hand.open @13:40:32.63 Homing MFB Syringe @13:40:32.93 AFV.select :holdingCoil @13:40:33.45 ATV.dial :air2 @13:40:35.10
SC.reconfigure SCloose @13:40:35.86 AS.reconfigure ASconfig @13:40:36.35 AS.home.seek :home @13:40:36.51
Elbow.reconfigure TightElbow @13:40:36.91 Elbow.seek 2 @13:40:38.27 Forearm.seek 2 + 700 counts @13:40:39.77 BACfans.email "Started mfb in tube 2 at 13:40:31CEST14-Jul-20\n{}\nCan@13:40:30, 23.5C, 52% humidity, 14.6psia, 11.962V, 0.809A, 0.809A avg, 9.67W",Subject: "pcrBAC wcr sampling at most 100ml" @13:40:39.98
SE.reconfigure SEpick @13:40:40.31 SE.seek beamBreak @13:40:42.33 AFV.select :carrierSolution @13:40:42.91 delay 2 seconds @13:40:43.99
SE.configure SEconfig @13:40:45.33 Elbow.seek 2 @13:40:45.71 AS.seek 1ml @13:40:46.94
Forearm.seek 2 + 300 counts @13:40:47.54 Forearm.reconfigure LooseForearm @13:40:47.82 Hand.close @13:40:48.98 SE.seek :down @13:40:50.00
Forearm.seek :retract @13:40:51.27 Elbow.seek CC @13:40:52.14 SE.coast @13:40:53.57
Elbow.reconfigure LooseElbow @13:40:54.15 Forearm.reconfigure TightForearm @13:40:54.63 Forearm.seek CC @13:40:57.50 CC.seek :closed @13:42:55.34 delay 1 second @13:42:55.53
Collection Clamp onSpring/offSpring for 6/30 samples, on/off torque 2.34 @13:42:55.56 Hand.open @13:42:56.25 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[6E:27->20] @13:42:56.32 I2C::UnexpectedReply in core -- I2C::Servo::Status3Reply[6E:27->20] @13:42:56.76 Forearm.reconfigure LooseForearm @13:42:56.95 Forearm.seek :retract @13:42:58.63 AFV.select :holdingCoil @13:42:59.38 delay 1 second @13:43:00.22
SC.coast @13:43:00.41 skipping prime and sample collection @13:43:00.47 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @13:43:00.53 Intake.select :close @13:43:00.92 CSV.dial :waste @13:43:01.22 CS.seek 0ml @13:43:01.48 ATV.dial :waste @13:43:01.89 CSV.dial :air @13:43:01.96
Calibrating pressure and temperature sensors @13:43:02.19 CTV.dial :air @13:43:02.32 SSV.dial :spit,avoiding: :exhaust @13:43:02.44 SS.reconfigure SSfast @13:43:02.71 SS.seek 5ml @13:43:03.16 CS.seek 10ml @13:43:03.25 AS.reconfigure ASnorm @13:43:03.61 AS.home.seek :empty @13:43:06.01 SSV.dial CBV @13:43:06.11
CBV.dial SSV @13:43:07.86 delay 3.8 seconds @13:43:12.07 SSV.dial :spit @13:43:13.72 SS.seek 0ml @13:43:16.78 SSV.dial CBV @13:43:18.44 SSV.dial CBV,avoiding: :exhaust @13:43:18.56
CBV.dial SSV @13:43:18.62 CTV.dial :air @13:43:18.72 delay 15 seconds @13:43:33.80 SSV.dial :spit,avoiding: :exhaust @13:43:35.55 CTV.dial CSV,avoiding: :intake @13:43:35.68
SS.reconfigure SSexhale @13:43:36.08 SS.seek 0ml @13:43:36.40 SSV.dial CBV,avoiding: :exhaust @13:43:38.26 CBV.dial between SSV and waste2 @13:43:39.04 CTV.dial CSV,avoiding: :air @13:43:39.13
CSV.dial CTV @13:43:39.86 CS.reconfigure CSpressurize @13:43:40.07 CS.seek 0ml @13:43:56.32 ATV.dial :waste @13:43:56.33 ARV.dial between carrier and air1 @13:43:56.46 ASV.dial :waste1 @13:44:06.40
CSV.dial :air @13:44:06.95 CS.reconfigure CSfast @13:44:07.03 CS.seek 10ml @13:44:33.69 CSV.dial between air and CTV @13:44:34.08 CS.seek 3.386ml @13:44:51.99 CTV.dial CSV,avoiding: :air @13:44:52.02
CSV.dial CTV @13:44:52.40 CS.reconfigure CSpressurize @13:44:52.49 CS.seek 0ml @13:45:02.01 CSV.dial :air @13:45:02.55 CS.reconfigure CSfast @13:45:02.63 CS.seek 10ml @13:45:29.16 CSV.dial between air and CTV @13:45:29.55 CS.seek 2.11ml @13:45:50.82 CTV.dial CSV,avoiding: :air @13:45:50.85
CSV.dial CTV @13:45:51.24 CS.reconfigure CSpressurize @13:45:51.33 CS.seek 0ml @13:45:52.68 BubblePoint: Intake @69.6psia, Outlet @14.4psia @13:45:52.70 CBV.dial SSV @13:45:53.10 SS.reconfigure SSconfig @13:45:53.19 SS.seek 10ml @13:45:59.40 delay 15 seconds @13:46:14.53 Evacuated: Intake @28.7psia, Outlet @29.1psia @13:46:14.55 SSV.dial :spit,avoiding: :exhaust @13:46:16.11 SS.reconfigure SSexhale @13:46:16.19 SS.seek 0ml @13:46:21.31 SS.reconfigure SSfast @13:46:21.39 SS.seek 5ml @13:46:24.29 SSV.dial CBV,avoiding: :exhaust @13:46:24.33
CBV.dial SSV @13:46:25.89 CTV.dial :air,avoiding: :intake @13:46:27.90 CSV.dial CTV @13:46:27.97
SS.seek 10ml @13:46:28.94 CSV.dial :air @13:46:29.48 CS.reconfigure CSfast @13:46:29.56 CS.seek 0ml @13:46:30.77
SSV.dial :spit,avoiding: :exhaust @13:46:32.29 SS.reconfigure SSexhale @13:46:32.38 SS.seek 0ml @13:46:35.31 CS.coast @13:46:37.61
SS.reconfigure SSfast @13:46:37.69 SS.seek 5ml @13:46:40.76 SSV.dial CBV,avoiding: :exhaust @13:46:40.79
CBV.dial SSV @13:46:43.36 SSV.dial :spit,avoiding: :exhaust @13:46:44.88 SS.reconfigure SSconfig @13:46:44.97 SS.seek 0ml @13:46:48.09 SS.coast @13:46:48.17 Evacuating Puck (bubblePt=55psi, evacVolume=10ml, delay 15 seconds) @13:46:48.18 Intake.select :close @13:46:48.30 CSV.dial :waste @13:46:48.48 SSV.dial CBV,avoiding: :exhaust @13:46:48.50
CBV.dial SSV @13:46:49.25 CS.seek 0ml @13:46:49.34 CSV.dial :air @13:46:50.05
CTV.dial :air @13:46:50.07 delay 15 seconds @13:46:50.35 CS.seek 10ml @13:47:05.11
SSV.dial :spit,avoiding: :exhaust @13:47:06.63 CTV.dial CSV,avoiding: :intake @13:47:06.68
SS.reconfigure SSexhale @13:47:06.82 SS.seek 0ml @13:47:06.92 SSV.dial CBV,avoiding: :exhaust @13:47:17.19 CBV.dial between SSV and waste2 @13:47:17.66 CTV.dial CSV,avoiding: :air @13:47:17.70
CSV.dial CTV @13:47:18.37 CS.reconfigure CSpressurize @13:47:18.45 CS.seek 0ml @13:47:44.67 CSV.dial :air @13:47:45.20 CS.reconfigure CSfast @13:47:45.28 CS.seek 10ml @13:48:11.98 CSV.dial between air and CTV @13:48:12.50 CS.seek 3.383ml @13:48:30.29 CTV.dial CSV,avoiding: :air @13:48:30.32
CSV.dial CTV @13:48:30.73 CS.reconfigure CSpressurize @13:48:30.81 CS.seek 0ml @13:48:40.32 CSV.dial :air @13:48:40.87 CS.reconfigure CSfast @13:48:40.95 CS.seek 10ml @13:49:07.57 CSV.dial between air and CTV @13:49:07.97 CS.seek 2.107ml @13:49:29.10 CTV.dial CSV,avoiding: :air @13:49:29.13
CSV.dial CTV @13:49:29.54 CS.reconfigure CSpressurize @13:49:29.61 CS.seek 0ml @13:49:31.06 BubblePoint: Intake @70.1psia, Outlet @15.0psia @13:49:31.07 CBV.dial SSV @13:49:31.49 SS.reconfigure SSconfig @13:49:31.57 SS.seek 10ml @13:49:37.63 delay 15 seconds @13:49:52.76 Evacuated: Intake @28.8psia, Outlet @29.1psia @13:49:52.78 SSV.dial :spit,avoiding: :exhaust @13:49:54.33 SS.reconfigure SSexhale @13:49:54.41 SS.seek 0ml @13:49:59.25 SS.reconfigure SSfast @13:49:59.33 SS.seek 5ml @13:50:02.40 SSV.dial CBV,avoiding: :exhaust @13:50:02.44
CBV.dial SSV @13:50:03.99 CTV.dial :air,avoiding: :intake @13:50:06.01 CSV.dial CTV @13:50:06.08
SS.seek 10ml @13:50:07.06 CSV.dial :air @13:50:07.60 CS.reconfigure CSfast @13:50:07.68 CS.seek 0ml @13:50:09.08
SSV.dial :spit,avoiding: :exhaust @13:50:10.62 SS.reconfigure SSexhale @13:50:10.71 SS.seek 0ml @13:50:13.31 CS.coast @13:50:16.09
SS.reconfigure SSfast @13:50:16.17 SS.seek 5ml @13:50:18.87 SSV.dial CBV,avoiding: :exhaust @13:50:18.90
CBV.dial SSV @13:50:21.46 SSV.dial :spit,avoiding: :exhaust @13:50:23.02 SS.reconfigure SSconfig @13:50:23.10 SS.seek 0ml @13:50:26.62 SS.coast @13:50:26.70 Lysing: Adding 1.5ml lysis @13:50:26.72 CBV.dial between CSV and waste1,avoiding: :DBV @13:50:28.49 CTV.dial CSV @13:50:30.03 CSV.dial CSR @13:50:30.96 Pulling 1ml of CSR.lysis @13:50:30.97 CSR.select :lysis @13:50:31.14 CS.reconfigure CSconfig @13:50:31.21 CS.seek 1ml @13:50:34.76 delay 5 seconds @13:50:39.79 CSR.select :air @13:50:40.00 CS.seek 6ml @13:50:53.82 delay 2 seconds @13:50:55.84 CSV.dial :waste1 @13:50:57.34 CS.reconfigure CSfast @13:50:57.42 CS.seek :empty @13:51:13.70 CSV.dial CSR @13:51:15.37 Pulling 1.5ml of CSR.lysis @13:51:15.38 CSR.select :lysis @13:51:15.55 CS.reconfigure CSconfig @13:51:15.62 CS.seek 1.5ml @13:51:20.48 delay 5 seconds @13:51:25.51 CSR.select :air @13:51:25.70 CS.seek 4.5ml @13:51:34.39 delay 2 seconds @13:51:36.41 CSV.dial CTV @13:51:36.94 CS.seek 1.275ml @13:51:45.71 -> exit @13:51:45.79 Session Ended @13:51:46.52
Heating sh1 puck from tube 2 in Collection Clamp to 85C for 5:00 @13:51:46.54 CH.seek 85C,8:00 @13:53:05.55 Session Begun @13:54:52.43
CH.hold 5:00,85C @13:59:52.63 Collection Puck Heater at 85.1C after lysing @13:59:52.66 CS.seek 0ml @13:59:57.22 CTV.dial between spare and air,avoiding: :intake @13:59:58.45 CSV.dial :air @13:59:58.98 CS.seek 5ml @14:00:12.86 CSV.dial CTV @14:00:13.39 delay 2 seconds @14:00:15.41 CSV.dial :air @14:00:15.94 delay 2 seconds @14:00:17.96 CSV.dial CTV @14:00:18.48 delay 2 seconds @14:00:20.50 CSV.dial :air @14:00:21.03 delay 2 seconds @14:00:23.06 CS.seek 0ml @14:00:36.83 Recovering Lysate into Collection syringe @14:00:36.84 CSV.dial CBV @14:00:38.05 CBV.dial CSV @14:00:38.49 CS.seek 5ml @14:00:52.27 delay 5 seconds @14:00:57.30 CTV.dial :air @14:00:57.90 CS.seek 7ml @14:01:04.27 delay 5 seconds @14:01:09.30 CSV.dial :air2 @14:01:09.82 CS.seek 10ml @14:01:18.58 CSV.dial between air and CTV,avoiding: CBV @14:01:19.92 Lysing Complete @14:01:20.02 Storing sh1 puck in Collection Clamp to tube 1 @14:01:20.23 SC.reconfigure SCtight @14:01:20.30 SC.seek 1 @14:01:22.47 SC.reconfigure SCloose @14:01:22.71 Elbow.reconfigure TightElbow @14:01:22.78 Elbow.seek 1 @14:01:24.72 Forearm.reconfigure TightForearm @14:01:24.80 Forearm.seek 1 + 700 counts @14:01:25.85 SE.reconfigure SEpick @14:01:25.95 SE.seek beamBreak @14:01:28.93 SE.configure SEconfig @14:01:29.01 SE.seek :place @14:01:29.56 Forearm.reconfigure LooseForearm @14:01:29.64 Forearm.seek :retract @14:01:30.58 Elbow.seek CC @14:01:32.28 Elbow.reconfigure LooseElbow @14:01:32.41 Forearm.seek CC @14:01:33.47 Hand.close @14:01:34.20 CC.seek :open @14:01:38.73 Forearm.seek :retract @14:01:39.78 Elbow.reconfigure TightElbow @14:01:39.85 Elbow.seek 1 @14:01:41.89 Elbow.reconfigure LimpElbow @14:01:41.97 Forearm.seek 1 @14:01:43.03 Elbow.hold @14:01:43.12 Elbow.reconfigure TightElbow @14:01:43.19 Hand.open @14:01:44.08 Forearm.force -20 @14:01:44.15 Forearm.seek 1 + 347 counts @14:01:45.02 Forearm.reconfigure TightForearm @14:01:45.10 Forearm.seek 1 + 700 counts @14:01:45.49 SE.seek :down @14:01:46.65
Forearm.reconfigure LooseForearm @14:01:46.72 Forearm.seek :retract @14:01:48.34 SE.coast @14:01:48.40
SC.coast @14:01:48.46 Tranferring lysate to PRV @14:01:48.47 CSV.dial :air @14:01:48.84 CS.seek 10ml @14:01:48.96 CSV.dial between air and CTV @14:01:49.33 AIV.dial :bypass @14:01:49.44 Beginning samplePassC @14:01:49.45 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:01:49.56 Hand.open @14:01:49.96 Elbow.seek FlushPuck::Garage @14:01:51.90 Elbow.reconfigure LooseElbow @14:01:52.03 Forearm.seek :garage @14:01:53.09 Hand.close @14:01:53.97 Forearm.seek :retract @14:01:55.03 Elbow.reconfigure TightElbow @14:01:55.10 Elbow.seek CC @14:01:58.00 Elbow.reconfigure LooseElbow @14:01:58.13 Forearm.reconfigure TightForearm @14:01:58.20 Forearm.seek CC @14:01:59.55 CC.seek :closed @14:02:03.92 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.35 @14:02:04.02 Hand.open @14:02:04.90 Forearm.reconfigure LooseForearm @14:02:04.97 Forearm.seek :retract @14:02:06.02 AWV.select :air @14:02:06.04 AIV.dial :bypass @14:02:06.06 PSV.dial PSR @14:02:07.64 Pulling 1.5ml of PSR.flush @14:02:07.65 PSR.select :flush @14:02:07.82 PS.seek 1.5ml @14:02:24.03 delay 5 seconds @14:02:29.06 PSV.dial :waste1 @14:02:29.63 PS.seek 0ml @14:02:45.93 PSV.dial PSR @14:02:46.47 PSR.select :air @14:02:46.62 PS.seek 2.5ml @14:03:13.22 PRV.dial CBV @14:03:13.25
CBV.dial PRV @14:03:14.19 PSV.dial :mixloop @14:03:15.38 CTV.dial :air,avoiding: :intake @14:03:15.39 PTV.dial :PRVmixing @14:03:15.52 PS.seek 0ml @14:03:42.21 PSV.dial :air @14:03:42.75 PS.seek 2.5ml @14:04:09.41 PSV.dial :mixloop @14:04:09.94 PS.seek 0ml @14:04:36.65 PSV.dial :air @14:04:37.20 PS.seek 2.5ml @14:05:03.58 PSV.dial :mixloop @14:05:04.12 PS.seek 0ml @14:05:30.75 Garaging FlushPuck in Collection Clamp @14:05:31.29 Elbow.reconfigure TightElbow @14:05:31.45 Elbow.seek CC @14:05:31.70 Elbow.reconfigure LooseElbow @14:05:31.83 Forearm.seek CC @14:05:32.93 Hand.close @14:05:33.71 CC.seek :open @14:05:38.15 Forearm.seek :retract @14:05:39.24 Elbow.reconfigure TightElbow @14:05:39.31 Elbow.seek :garage @14:05:42.36 Elbow.reconfigure LooseElbow @14:05:42.49 Forearm.reconfigure TightForearm @14:05:42.57 Forearm.seek :garage @14:05:43.74 Hand.open @14:05:44.53 Forearm.reconfigure LooseForearm @14:05:44.60 Forearm.seek :retract @14:05:45.69 Connecting PS to CS to transfer sample @14:05:45.70 CBV.dial AIV @14:05:45.73 AIV.dial :bypass @14:05:45.76 PSV.dial :mixloop @14:05:45.78 PTV.dial :PRVmixing @14:05:45.81 PRV.dial AIV @14:05:45.84 CTV.dial :air,avoiding: :intake @14:05:45.88 Loading lyfil puck from tube 2 into Collection Clamp @14:05:46.15 SC.reconfigure SCtight @14:05:46.22 SC.seek 2 @14:05:48.41 SC.reconfigure SCloose @14:05:48.65 Elbow.reconfigure TightElbow @14:05:48.72 Elbow.seek 2 @14:05:50.95 Forearm.reconfigure TightForearm @14:05:51.03 Forearm.seek 2 + 700 counts @14:05:52.14 SE.reconfigure SEpick @14:05:52.24 SE.seek beamBreak @14:05:55.37 SE.configure SEconfig @14:05:55.56 Elbow.seek 2 @14:05:55.77 Forearm.seek 2 + 300 counts @14:05:56.07 Forearm.reconfigure LooseForearm @14:05:56.13 Hand.close @14:05:56.98 SE.seek :down @14:05:57.21
Forearm.seek :retract @14:05:58.18 Elbow.seek CC @14:05:59.85 SE.coast @14:05:59.89
Elbow.reconfigure LooseElbow @14:06:00.04 Forearm.reconfigure TightForearm @14:06:00.11 Forearm.seek CC @14:06:01.46 CC.seek :closed @14:06:05.74 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.32 @14:06:05.75 Hand.open @14:06:06.65 Forearm.reconfigure LooseForearm @14:06:06.71 Forearm.seek :retract @14:06:07.74 SC.coast @14:06:07.79 CTV.dial CSV,avoiding: :intake @14:06:09.35 CSV.dial CTV @14:06:09.72 CS.seek 5ml @14:06:23.64 delay 1:20 @14:07:43.68 CS.seek 2.5ml @14:07:51.18 delay 40 seconds @14:08:31.21 CS.seek 0ml @14:08:38.71 delay 1:00 @14:09:38.74 PSV.dial :air @14:09:39.79 PS.seek 2.5ml @14:10:06.21 PSV.dial :mixloop @14:10:06.75 delay 3 seconds @14:10:09.78 PSV.dial :air @14:10:10.30 delay 2 seconds @14:10:12.32 PSV.dial :mixloop @14:10:12.85 delay 3 seconds @14:10:15.88 PSV.dial :waste1 @14:10:17.40 PS.seek :empty @14:10:43.99 CBV.dial :waste1 @14:10:44.55 delay 5 seconds @14:10:49.58 CBV.dial AIV @14:10:50.13 delay 10 seconds @14:11:00.16 CBV.dial :waste1 @14:11:00.71 delay 10 seconds @14:11:10.74 CBV.dial AIV @14:11:11.29 delay 10 seconds @14:11:21.32 CTV.dial CSV,avoiding: :intake @14:11:21.33 CBV.dial between AIV and waste1 @14:11:21.74 CSV.dial :air @14:11:22.30 CS.seek 10ml @14:11:49.14 CSV.dial CTV @14:11:49.70 CS.seek 0ml @14:11:59.82 Session Begun @14:12:16.96
CBV.dial :waste1 @14:12:17.35 delay 10 seconds @14:12:27.38 CTV.dial :air,avoiding: :intake @14:12:29.03 Storing lyfil puck in Collection Clamp to tube 1 @14:12:29.26 SC.reconfigure SCtight @14:12:29.35 SC.seek 1 @14:12:31.54 SC.reconfigure SCloose @14:12:31.80 Elbow.reconfigure TightElbow @14:12:31.88 Elbow.seek 1 @14:12:33.81 Forearm.reconfigure TightForearm @14:12:33.90 Forearm.seek 1 + 700 counts @14:12:34.97 SE.reconfigure SEpick @14:12:35.09 SE.seek beamBreak @14:12:38.17 SE.configure SEconfig @14:12:38.38 Elbow.reconfigure LimpElbow @14:12:38.47 Forearm.reconfigure LooseForearm @14:12:38.56 Forearm.seek 1 @14:12:38.91 Elbow.hold @14:12:39.03 Elbow.reconfigure TightElbow @14:12:39.16 SE.seek 0.06 Pucks below Top Plate @14:12:39.66 Forearm.force -20 @14:12:39.74 Forearm.seek 1 + 349 counts @14:12:40.10 Forearm.reconfigure TightForearm @14:12:40.19 Forearm.seek 1 + 700 counts @14:12:40.43 SE.seek 1.06 Pucks below Top Plate @14:12:41.16 Forearm.reconfigure LooseForearm @14:12:41.24 Forearm.seek :retract @14:12:42.20 Elbow.seek CC @14:12:44.00 Elbow.reconfigure LooseElbow @14:12:44.14 Forearm.seek CC @14:12:45.24 Hand.close @14:12:45.97 CC.seek :open @14:12:50.52 Forearm.seek :retract @14:12:51.61 Elbow.reconfigure TightElbow @14:12:51.70 Elbow.seek 1 @14:12:54.16 Elbow.reconfigure LimpElbow @14:12:54.25 Forearm.seek 1 @14:12:55.37 Elbow.hold @14:12:55.48 Elbow.reconfigure TightElbow @14:12:55.57 Hand.open @14:12:56.47 Forearm.force -20 @14:12:56.56 Forearm.seek 1 + 345 counts @14:12:56.91 Forearm.reconfigure TightForearm @14:12:57.00 Forearm.seek 1 + 700 counts @14:12:57.53 SE.seek :down @14:12:58.68
Forearm.reconfigure LooseForearm @14:12:58.77 Forearm.seek :retract @14:13:00.28 SE.coast @14:13:00.36
SC.coast @14:13:00.44 PSV.dial :air @14:13:01.81 PS.seek 0.499ml @14:13:07.88 delay 3 seconds @14:13:10.90 PSV.dial :mixloop @14:13:11.44 PRV.dial :air @14:13:12.00 delay 3 seconds @14:13:15.03 PRV.dial CBV @14:13:15.58 CBV.dial AIV @14:13:16.15 PRV.dial between CBV and air @14:13:16.53 PRV.dial :air @14:13:16.98 PS.reconfigure PSslow @14:13:17.07 PS.seek 0.599ml @14:13:19.65 PRV.dial :waste2 @14:13:20.52 PS.reconfigure PSconfig @14:13:20.60 PS.seek :empty @14:13:27.60 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:13:27.71 Hand.open @14:13:28.10 Elbow.seek FlushPuck::Garage @14:13:30.06 Elbow.reconfigure LooseElbow @14:13:30.20 Forearm.seek :garage @14:13:31.31 Hand.close @14:13:32.22 Forearm.seek :retract @14:13:33.32 Elbow.reconfigure TightElbow @14:13:33.41 Elbow.seek CC @14:13:36.34 Elbow.reconfigure LooseElbow @14:13:36.49 Forearm.reconfigure TightForearm @14:13:36.57 Forearm.seek CC @14:13:37.97 CC.seek :closed @14:13:42.33 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.42 @14:13:42.44 Hand.open @14:13:43.29 Forearm.reconfigure LooseForearm @14:13:43.38 Forearm.seek :retract @14:13:45.04 Clearing PML for modified lysate @14:13:45.06 CTV.dial :air,avoiding: :intake @14:13:45.12 PRV.dial :waste2 @14:13:45.14 PTV.dial :PRV,avoiding: :puck @14:13:46.03 delay 2 seconds @14:13:48.05 PTV.dial :PRVmixing,avoiding: :puck @14:13:48.92 delay 2 seconds @14:13:50.94 PSV.dial :PML @14:13:50.96 delay 2 seconds @14:13:52.99 PRV.dial :air @14:13:53.86 delay 2 seconds @14:13:55.94 PS.seek 1.499ml @14:14:12.33 delay 5 seconds @14:14:17.36 PS.seek 2.5ml @14:14:28.52 PSV.dial :air @14:14:29.09 PS.seek 0ml @14:14:55.69 mixing lysate with speDil @14:14:55.70 PSV.dial :PML @14:14:56.29 CBV.dial :PRV @14:14:56.30 PRV.dial :air @14:14:56.35 delay 2 seconds @14:14:58.40 PRV.dial :speDil @14:14:58.94 delay 2 seconds @14:15:01.02 PS.reconfigure PSslow1 @14:15:01.11 PS.seek 0.225ml @14:15:25.13 delay 5 seconds @14:15:30.16 PRV.dial CBV @14:15:31.02 delay 2 seconds @14:15:33.10 PS.seek 0.475ml @14:15:58.88 delay 5 seconds @14:16:03.91 PRV.dial :air @14:16:04.46 delay 2 seconds @14:16:06.49 PS.seek 0.965ml @14:16:56.60 delay 10 seconds @14:17:06.63 PRV.dial CBV @14:17:07.18 CTV.dial :air,avoiding: :intake @14:17:07.20 PSV.dial :air @14:17:07.77 PS.reconfigure PSconfig @14:17:07.87 PS.seek 2.5ml @14:17:24.22 delay 3 seconds @14:17:27.24 PSV.dial PTV @14:17:28.10 PTV.dial :PRV,avoiding: :puck @14:17:28.97 PS.seek 0ml @14:17:55.13 PSV.dial :air @14:17:56.01 PS.seek 2.5ml @14:18:22.38 delay 3 seconds @14:18:25.40 PSV.dial PTV @14:18:26.36 PTV.dial :PRV,avoiding: :puck @14:18:26.39 PS.seek 0ml @14:18:52.69 Pushing modified lysate into AIV Injection Loop @14:18:52.70 CBV.dial AIV @14:18:52.76 AIV.dial :bypass @14:18:52.78 PRV.dial CBV @14:18:52.84 PSV.dial :air @14:18:53.73 PS.seek 2.5ml @14:19:20.12 delay 3 seconds @14:19:23.22 PSV.dial :PML @14:19:23.75 PTV.dial :PRVmixing,avoiding: :puck @14:19:24.65 PS.reconfigure PSslow1 @14:19:24.73 PS.seek 1.74ml @14:20:42.19 delay 5 seconds @14:20:47.22 AIV.dial :sample @14:20:47.92 AWV.select :waste @14:20:48.13 PS.reconfigure PSslow2 @14:20:48.22 PS.seek 1.22ml @14:22:36.44 delay 15 seconds @14:22:51.47 AIV.dial :bypass @14:22:52.12 PS.reconfigure PSconfig @14:22:52.21 PS.seek 0ml @14:23:05.51 PSV.dial :air @14:23:06.06 PS.seek 2.5ml @14:23:32.65 PSV.dial :PML @14:23:33.22 PS.seek 0ml @14:23:59.46 AIV.dial :coilToMFB @14:23:59.48 AWV.select :air @14:23:59.71 Extracting Nucleic Acid from AIV @14:24:00.25
Beginning samplePassD @14:24:00.30 SPE.hold 2:46:40,55C @14:24:00.45 ASV.dial :SPEdelivery @14:24:00.56
AWV.select :air @14:24:00.58 AIV.dial :bypass @14:24:00.63 PSV.dial PSR @14:24:00.91 delay 20 seconds @14:24:01.97
Pulling 1.5ml of PSR.killMFB @14:24:01.98 PSR.select :killMFB @14:24:02.17 PS.seek 1.5ml @14:24:19.29 delay 5 seconds @14:24:20.94 primeLysateINT @14:24:20.95 AWV.select :air @14:24:21.00 AIV.dial :coilToMFB @14:24:21.04 ATV.dial AIV @14:24:23.07 AS.reconfigure ASslow1 @14:24:23.16 AS.seek 0.299ml @14:24:24.32
PSV.dial :waste1 @14:24:24.89 PS.seek 0ml @14:24:41.97 PSV.dial PSR @14:24:42.57 PSR.select :air @14:24:42.78 PS.seek 2.5ml @14:25:10.85 PRV.dial CBV @14:25:10.89
CBV.dial PRV @14:25:10.94 PSV.dial :mixloop @14:25:12.19 CTV.dial :air,avoiding: :intake @14:25:12.21 PTV.dial :PRVmixing @14:25:12.27 PS.seek 0ml @14:25:23.78 delay 10 seconds @14:25:33.81 ATV.dial :air2 @14:25:34.78 AS.seek 0.329ml @14:25:40.29
PSV.dial PSR @14:25:41.12 ATV.dial :waste2 @14:25:41.60
Pulling 1.5ml of PSR.flush @14:25:41.61 PSR.select :flush @14:25:41.78 AS.reconfigure ASnorm @14:25:41.87 AS.seek :empty @14:25:41.97
PS.seek 1.5ml @14:25:58.94 Lysate primed! @14:25:58.95 AFV.select :holdingCoil @14:25:59.00 ATV.dial :air2 @14:25:59.11
delay 5 seconds @14:25:59.73 AS.reconfigure ASslow1 @14:25:59.81 AS.seek 0.069ml @14:26:04.14
PSV.dial :waste1 @14:26:04.72 PS.seek 0ml @14:26:14.24 delay 3 seconds @14:26:17.27 ATV.dial :AIV @14:26:18.23 AS.seek 0.469ml @14:26:21.68
PSV.dial PSR @14:26:22.27 PSR.select :air @14:26:22.44 PS.seek 2.5ml @14:26:50.19 PRV.dial CBV @14:26:50.23
CBV.dial PRV @14:26:50.28 PSV.dial :mixloop @14:26:51.51 CTV.dial :air,avoiding: :intake @14:26:51.53 PTV.dial :PRVmixing @14:26:51.59 PS.seek 0ml @14:27:19.10 PSV.dial PSR @14:27:20.39 Pulling 1.5ml of PSR.flush @14:27:20.41 PSR.select :flush @14:27:20.59 PS.seek 1.5ml @14:27:37.32 delay 5 seconds @14:27:38.89 delay 5 seconds @14:27:42.35
PSV.dial :waste1 @14:27:42.92 PS.seek 0ml @14:27:43.96 Solid Phase Extraction Heater at 50.4C @14:27:43.97 ATV.dial ASV @14:27:44.44 ASV.dial :SPEdelivery @14:27:44.56 AS.seek 0.041ml @14:27:59.59
PSV.dial PSR @14:28:00.16 PSR.select :air @14:28:00.33 PS.seek 2.5ml @14:28:27.60 PRV.dial CBV @14:28:27.64
CBV.dial PRV @14:28:27.69 PSV.dial :mixloop @14:28:28.91 CTV.dial :air,avoiding: :intake @14:28:28.93 PTV.dial :PRVmixing @14:28:29.52 PS.seek 0ml @14:28:56.63 PSV.dial PSR @14:28:57.91 Pulling 1.5ml of PSR.flush @14:28:57.92 PSR.select :flush @14:28:58.11 PS.seek 1.5ml @14:29:10.92 delay 10 seconds @14:29:14.57
delay 5 seconds @14:29:19.60 PSV.dial :waste1 @14:29:20.16 PS.seek 0ml @14:29:20.94 rinseLysateLineINT @14:29:20.95 AFV.select :carrierSolution @14:29:21.11 delay 2 seconds @14:29:23.14 AS.reconfigure ASconfig @14:29:23.23 AS.seek 1ml @14:29:36.57
PSV.dial PSR @14:29:37.13 PSR.select :air @14:29:37.30 PS.seek 2.5ml @14:30:04.23 PRV.dial CBV @14:30:04.27
CBV.dial PRV @14:30:04.32 PSV.dial :mixloop @14:30:05.54 CTV.dial :air,avoiding: :intake @14:30:05.56 PTV.dial :PRVmixing @14:30:05.62 PS.reconfigure PSslow @14:30:05.71 PS.seek 1.2ml @14:30:18.64 delay 1 second @14:30:19.66 AFV.select :holdingCoil @14:30:19.79 delay 1 second @14:30:20.82 ATV.dial AIV @14:30:21.41 AIV.dial :coilToMFB @14:30:21.43 AWV.select :waste @14:30:21.58 AS.reconfigure ASslow0 @14:30:21.66 AS.seek 0ml @14:30:32.54
PSV.dial :waste1 @14:30:34.09 PS.reconfigure PSconfig @14:30:34.18 PS.seek 0ml @14:30:47.48 PSV.dial :air @14:30:48.81 PS.seek 2.5ml @14:31:15.48 PSV.dial :mixloop @14:31:16.02 PS.reconfigure PSslow @14:31:16.11 PS.seek 0ml @14:32:02.60 ATV.dial :air2 @14:32:03.34 AS.reconfigure ASslow1 @14:32:03.42 AS.seek 0.05ml @14:32:07.26
PSV.dial :air @14:32:07.80 PS.reconfigure PSconfig @14:32:07.90 PS.seek 2.5ml @14:32:13.77 ATV.dial :AIV @14:32:14.67 AS.seek 0.01ml @14:32:23.05 ATV.dial :waste @14:32:24.80 AS.seek :empty @14:32:27.27 ethanol wash @14:32:27.28 ATV.dial :air2 @14:32:28.66 AS.reconfigure ASslow2 @14:32:28.80 AS.seek 0.009ml @14:32:34.63
PSV.dial :mixloop @14:32:35.16 PS.reconfigure PSslow @14:32:35.26 PS.seek 0ml @14:32:39.33 delay 3 seconds @14:32:42.36 Pulling 0.4ml of Ethanol Wash @14:32:42.37 ATV.dial :ethanol @14:32:43.29 AS.reconfigure ASslow1 @14:32:43.37 AS.seek 0.409ml @14:33:26.35
Cleaning Collection Syringe @14:33:26.37 CSV.dial CSR @14:33:26.98 Pulling 4ml of CSR.flush @14:33:27.00 CSR.select :flush @14:33:27.26 CS.seek 4ml @14:33:38.78 delay 5 seconds @14:33:43.81 CSR.select :air @14:33:43.98 CS.seek 10ml @14:34:00.56 CSV.dial CTV @14:34:00.60
CTV.dial CSV @14:34:02.25 CBV.dial :waste1 @14:34:02.84 CS.seek 0ml @14:34:04.17 delay 3 seconds @14:34:07.19 ATV.dial :air1 @14:34:07.63 delay 3 seconds @14:34:10.72 AS.seek 0.434ml @14:34:16.05 delay 3 seconds @14:34:19.08 ATV.dial :ethanol @14:34:20.25 AS.seek 0.419ml @14:34:23.58 delay 3 seconds @14:34:26.61 ATV.dial ASV @14:34:28.03 AS.seek 0.092ml @14:34:29.63
CSV.dial CSR @14:34:30.26 Pulling 4ml of CSR.flush @14:34:30.27 CSR.select :flush @14:34:30.47 CS.seek 4ml @14:34:41.88 delay 5 seconds @14:34:46.92 CSR.select :air @14:34:47.09 CS.seek 10ml @14:35:03.63 CSV.dial CTV @14:35:03.67
CTV.dial CSV @14:35:04.25 CBV.dial :waste1 @14:35:04.30 CS.seek 0ml @14:35:31.17 Garaging FlushPuck in Collection Clamp @14:35:31.29 Elbow.reconfigure TightElbow @14:35:31.37 Elbow.seek CC @14:35:31.67 Elbow.reconfigure LooseElbow @14:35:31.81 Forearm.seek CC @14:35:32.87 Hand.close @14:35:33.62 CC.seek :open @14:35:34.08 delay 3 seconds @14:35:37.10 ATV.dial :waste2 @14:35:37.89 AS.reconfigure ASconfig @14:35:38.01 AS.seek :empty @14:35:38.22
Forearm.seek :retract @14:35:39.30 Elbow.reconfigure TightElbow @14:35:39.39 Elbow.seek :garage @14:35:42.58 Elbow.reconfigure LooseElbow @14:35:42.73 Forearm.reconfigure TightForearm @14:35:42.81 Forearm.seek :garage @14:35:43.73 ATV.dial :air2 @14:35:44.00
Hand.open @14:35:44.17 AS.reconfigure ASslow1 @14:35:44.25 AS.seek 1ml @14:35:44.81
Forearm.reconfigure LooseForearm @14:35:44.90 Forearm.seek :retract @14:35:45.97 Whole Cell Archive sampling 100ml @14:35:46.00 Loading wcr puck from tube 2 into Collection Clamp @14:35:46.31 SC.reconfigure SCtight @14:35:46.41 SC.seek 2 @14:35:48.63 SC.reconfigure SCloose @14:35:48.90 Elbow.reconfigure TightElbow @14:35:48.99 Elbow.seek 2 @14:35:51.15 Forearm.reconfigure TightForearm @14:35:51.25 Forearm.seek 2 + 700 counts @14:35:52.34 SE.reconfigure SEpick @14:35:52.46 SE.seek beamBreak @14:35:55.61 SE.configure SEconfig @14:35:55.81 Elbow.seek 2 @14:35:56.06 Forearm.seek 2 + 300 counts @14:35:56.37 Forearm.reconfigure LooseForearm @14:35:56.45 Hand.close @14:35:57.26 SE.seek :down @14:35:58.06 Forearm.seek :retract @14:35:59.04 Elbow.seek CC @14:36:00.32 SE.coast @14:36:00.68 Elbow.reconfigure LooseElbow @14:36:00.83 Forearm.reconfigure TightForearm @14:36:00.91 Forearm.seek CC @14:36:02.32 CC.seek :closed @14:36:06.65 Collection Clamp onSpring/offSpring for 6/31 samples, on/off torque 2.42 @14:36:06.67 Hand.open @14:36:07.56 Forearm.reconfigure LooseForearm @14:36:07.65 Forearm.seek :retract @14:36:08.73 SC.coast @14:36:08.80 collectSample 100ml w/bubble point of 55psi @14:36:08.83 Priming 7ml @14:36:08.86 Intake.select :close @14:36:08.90 Exhaust.select :close @14:36:08.95 SS.hold @14:36:09.24 Calibrating pressure sensors @14:36:09.25 CTV.dial :air @14:36:10.87 SSV.dial :spit,avoiding: :exhaust @14:36:10.92 SS.reconfigure SSfast @14:36:11.01 SS.seek 5ml @14:36:13.82 SSV.dial CBV @14:36:13.86 CBV.dial SSV @14:36:15.57 delay 3.8 seconds @14:36:19.51 SSV.dial :spit @14:36:21.06 SS.seek 0ml @14:36:24.21 SSV.dial CBV @14:36:25.78 CTV.dial :intake @14:36:26.69 SSV.dial CBV @14:36:26.73 CBV.dial SSV @14:36:26.78 Allowing 40s for 7.0ml of remaining 7.0ml @14:36:26.82 Intake.select :open @14:36:29.05 Intake @15.6psia, vacuum from 9.1 to 13.1psi @14:36:29.08 SS.reconfigure SSinhale.with maxSpeed: 150 @14:36:29.19 SS.seek 7ml @14:36:34.68 Stroke completed -- waiting for pressure to equalize ... @14:36:56.71 Intake.select :close @14:36:56.91 SSV.dial :exhaust @14:36:57.82 Exhaust.select :open @14:36:57.97 SS.reconfigure SSexhale @14:36:58.06 SS.seek 0ml @14:37:02.02 Exhaust.select :close @14:37:02.17 Sampling 100.0ml @14:37:02.19 Intake.select :close @14:37:02.21 Exhaust.select :close @14:37:02.25 SS.reconfigure SSconfig @14:37:02.38 SS.hold @14:37:02.64 CTV.dial :intake @14:37:02.67 SSV.dial CBV @14:37:02.72 CBV.dial SSV @14:37:03.65 Allowing 130s for 25.0ml of remaining 100.0ml @14:37:03.69 Intake.select :open @14:37:05.92 Intake @15.6psia, vacuum from 9.1 to 13.1psi @14:37:05.95 SS.reconfigure SSinhale.with maxSpeed: 150 @14:37:06.06 SS.seek 25ml @14:37:10.63 Vacuum too high -- waiting up to 40s @14:37:19.60 Intake @15.3psia, vacuum from 8.8 to 12.8psi @14:37:19.63 SS.reconfigure SSinhale.with maxSpeed: 135 @14:37:19.74 SS.seek 25ml @14:37:23.44 Vacuum too high -- waiting up to 40s @14:37:35.43 Intake @15.5psia, vacuum from 9.0 to 13.0psi @14:37:35.46 SS.reconfigure SSinhale.with maxSpeed: 122 @14:37:35.57 SS.seek 25ml @14:37:39.48 Vacuum too high -- waiting up to 40s @14:37:52.13 SS.reconfigure SSinhale.with maxSpeed: 110 @14:37:52.22 SS.seek 25ml @14:37:56.34 Vacuum too high -- waiting up to 40s @14:38:09.70 Intake @15.5psia, vacuum from 9.0 to 13.0psi @14:38:09.73 SS.reconfigure SSinhale.with maxSpeed: 99 @14:38:09.84 SS.seek 25ml @14:38:14.63 Vacuum too high -- waiting up to 40s @14:38:29.28 Intake @15.6psia, vacuum from 9.1 to 13.1psi @14:38:29.31 SS.reconfigure SSinhale.with maxSpeed: 89 @14:38:29.42 SS.seek 25ml @14:38:31.31 Stroke completed -- waiting for pressure to equalize ... @14:38:38.92 Intake.select :close @14:38:39.14 SSV.dial :exhaust @14:38:40.05 Exhaust.select :open @14:38:40.20 SS.reconfigure SSexhale @14:38:40.29 SS.seek 0ml @14:38:49.87 Exhaust.select :close @14:38:50.60 SSV.dial CBV @14:38:50.63 CBV.dial SSV @14:38:51.58 Allowing 130s for 25.0ml of remaining 75.0ml @14:38:51.60 Intake.select :open @14:38:53.85 Intake @15.5psia, vacuum from 9.0 to 13.0psi @14:38:53.88 SS.reconfigure SSinhale.with maxSpeed: 89 @14:38:53.99 SS.seek 25ml @14:38:58.53 Vacuum too high -- waiting up to 40s @14:39:05.83 delay 5 seconds @14:39:07.06 Intake @15.9psia, vacuum from 9.4 to 13.4psi @14:39:07.09 SS.reconfigure SSinhale.with maxSpeed: 80 @14:39:07.20 SS.seek 25ml @14:39:10.85 ATV.dial ASV @14:39:10.96 Vacuum too high -- waiting up to 40s @14:39:11.95 ASV.dial :SPEdelivery @14:39:12.07 AS.seek 0.02ml @14:39:18.38 Intake @15.8psia, vacuum from 9.3 to 13.3psi @14:39:18.44 SS.reconfigure SSinhale.with maxSpeed: 72 @14:39:18.83 SS.seek 25ml @14:39:23.19 Vacuum too high -- waiting up to 40s @14:39:31.88 SS.reconfigure SSinhale.with maxSpeed: 65 @14:39:32.16 SS.seek 25ml @14:39:37.95 Vacuum too high -- waiting up to 40s @14:39:47.19 Intake @15.9psia, vacuum from 9.4 to 13.4psi @14:39:47.22 SS.reconfigure SSinhale.with maxSpeed: 59 @14:39:47.33 SS.seek 25ml @14:39:53.22 Vacuum too high -- waiting up to 40s @14:40:02.39 Intake @15.8psia, vacuum from 9.3 to 13.3psi @14:40:02.99 SS.reconfigure SSinhale.with maxSpeed: 53 @14:40:03.10 SS.seek 25ml @14:40:10.31 Stroke completed -- waiting for pressure to equalize ... @14:40:23.69 Intake.select :close @14:40:23.88 SSV.dial :exhaust @14:40:24.80 Exhaust.select :open @14:40:24.95 SS.reconfigure SSexhale @14:40:25.04 SS.seek 0ml @14:40:34.75 Exhaust.select :close @14:40:34.90 SSV.dial CBV @14:40:34.94 CBV.dial SSV @14:40:35.85 Allowing 130s for 25.0ml of remaining 50.0ml @14:40:35.89 Intake.select :open @14:40:38.10 Intake @15.5psia, vacuum from 9.0 to 13.0psi @14:40:38.13 SS.reconfigure SSinhale.with maxSpeed: 53 @14:40:38.25 SS.seek 25ml @14:40:44.59 Vacuum too high -- waiting up to 40s @14:40:50.50 Intake @15.7psia, vacuum from 9.2 to 13.2psi @14:40:50.53 SS.reconfigure SSinhale.with maxSpeed: 48 @14:40:50.64 SS.seek 25ml @14:40:57.28 Vacuum too high -- waiting up to 40s @14:41:03.57 Intake @15.8psia, vacuum from 9.3 to 13.3psi @14:41:03.60 SS.reconfigure SSinhale.with maxSpeed: 43 @14:41:03.72 SS.seek 25ml @14:41:12.87 Vacuum too high -- waiting up to 40s @14:41:19.87 Intake @15.8psia, vacuum from 9.3 to 13.3psi @14:41:19.90 SS.reconfigure SSinhale.with maxSpeed: 39 @14:41:20.01 SS.seek 25ml @14:41:32.60 Vacuum too high -- waiting up to 40s @14:41:40.16 Intake @15.7psia, vacuum from 9.2 to 13.2psi @14:41:40.19 SS.reconfigure SSinhale.with maxSpeed: 35 @14:41:40.31 SS.seek 25ml @14:41:50.45 Stroke completed -- waiting for pressure to equalize ... @14:42:00.56 Intake.select :close @14:42:00.76 SSV.dial :exhaust @14:42:01.68 Exhaust.select :open @14:42:01.83 SS.reconfigure SSexhale @14:42:01.92 SS.seek 0ml @14:42:11.57 Exhaust.select :close @14:42:11.75 SSV.dial CBV @14:42:11.78 CBV.dial SSV @14:42:12.71 Allowing 130s for 25.0ml of remaining 25.0ml @14:42:12.75 Intake.select :open @14:42:14.97 Intake @15.5psia, vacuum from 9.0 to 13.0psi @14:42:15.00 SS.reconfigure SSinhale.with maxSpeed: 35 @14:42:15.10 SS.seek 25ml @14:42:29.46 delay 5 seconds @14:42:34.22 Vacuum too high -- waiting up to 40s @14:42:34.50 ATV.dial :air2 @14:42:35.63 AS.seek 0.52ml @14:42:41.09 Intake @15.7psia, vacuum from 9.2 to 13.2psi @14:42:41.13 SS.reconfigure SSinhale.with maxSpeed: 32 @14:42:41.24 SS.seek 25ml @14:43:16.07 Stroke completed -- waiting for pressure to equalize ... @14:43:30.01 Intake.select :close @14:43:30.22 SSV.dial :exhaust @14:43:31.14 Exhaust.select :open @14:43:31.29 SS.reconfigure SSexhale @14:43:31.38 SS.seek 0ml @14:43:40.77 Exhaust.select :close @14:43:40.93 Sampled 100.0ml @14:43:40.96 Drying puck before fixing for 4:00 @14:43:40.99 CBV.dial :waste2b @14:43:41.94 delay 1:00 @14:44:17.11 delay 5 seconds @14:44:22.14 ATV.dial ASV @14:44:23.19 ASV.dial :wasteR @14:44:23.59 AS.seek 0.3ml @14:44:41.97 CSV.dial :air @14:44:42.52 CS.reconfigure CSfast @14:44:42.61 CS.seek 10ml @14:45:08.16 delay 20 seconds @14:45:09.48 Pumping wcr puck from tube 2 up to 55psi... @14:45:09.50 CBV.dial SSV @14:45:11.57 CBV.dial between CSV and waste2b @14:45:12.75 CSV.dial between air and CTV @14:45:13.29 CS.seek 7.245ml @14:45:21.49 CTV.dial CSV,avoiding: :air @14:45:21.52 CSV.dial CTV @14:45:22.48 CS.reconfigure CSpressurize @14:45:22.58 CS.seek 0ml @14:45:28.20 AS.seek 0.07ml @14:45:39.53 CBV.dial :waste2b @14:45:39.95 CS.reconfigure CSconfig @14:45:40.04 CS.seek :empty @14:45:43.60 delay 1:00 @14:46:14.75 delay 20 seconds @14:46:34.78 ATV.dial :waste2 @14:46:35.58 AS.reconfigure ASconfig @14:46:35.67 AS.seek :empty @14:46:40.07 Elution @14:46:40.08 ATV.dial :air1 @14:46:41.31 AS.reconfigure ASslow1 @14:46:41.39 AS.seek 0.049ml @14:46:43.63 CTV.dial :air,avoiding: :intake @14:46:45.23 Fix #1 of 2 (with rnal) for 2:00 @14:46:45.25 CSV.dial CSR @14:46:45.87 Pulling 2ml of CSR.rnal @14:46:45.89 CSR.select :rnal @14:46:46.07 CS.seek 2ml @14:46:51.77 delay 3 seconds @14:46:52.26 delay 5 seconds @14:46:54.80 ATV.dial :ElutionWater @14:46:55.54 AS.seek 0.109ml @14:46:57.29 CSR.select :air @14:46:57.45 CS.reconfigure CSfast @14:46:57.54 CS.seek 10ml @14:47:07.92 delay 3 seconds @14:47:10.95 ATV.dial ASV,via: :air2 @14:47:13.15 ASV.dial :SPEelution @14:47:13.77 AFV.select :carrierSolution @14:47:13.95 AS.reconfigure ASnorm @14:47:14.03 AS.seek 0.759ml @14:47:19.23 Pumping wcr puck from tube 2 up to 55psi... @14:47:19.25 CBV.dial SSV @14:47:20.74 CBV.dial between CSV and waste2b @14:47:21.92 CSV.dial between air and CTV @14:47:22.66 CS.seek 7.168ml @14:47:31.04 CTV.dial CSV,avoiding: :air @14:47:31.08 CSV.dial CTV @14:47:32.71 CS.reconfigure CSpressurize @14:47:32.81 CS.seek 0ml @14:47:47.11 delay 3 seconds @14:47:50.14 AFV.select :holdingCoil @14:47:50.33 AS.reconfigure ASslow1 @14:47:50.42 AS.seek 0.474ml @14:47:51.00 delay 2:00 @14:48:48.03 delay 5 seconds @14:48:53.12 AS.reconfigure ASslow2 @14:48:53.22 AS.seek 0.439ml @14:49:28.65 delay 1:00 @14:49:51.03 CBV.dial :waste2b @14:49:51.45 CS.reconfigure CSconfig @14:49:51.55 CS.seek :empty @14:49:53.73 delay 1:00 @14:50:28.69 pushing eluate to holding coil @14:50:28.80 AS.seek 0.279ml @14:50:53.76 CTV.dial :air,avoiding: :intake @14:50:55.87 Fix #2 of 2 (with rnal) for 2:00 @14:50:55.89 CSV.dial CSR @14:50:56.52 Pulling 2ml of CSR.rnal @14:50:56.54 CSR.select :rnal @14:50:56.73 CS.seek 2ml @14:51:02.92 delay 5 seconds @14:51:07.96 CSR.select :air @14:51:08.12 CS.reconfigure CSfast @14:51:08.21 CS.seek 10ml @14:51:30.04 Pumping wcr puck from tube 2 up to 55psi... @14:51:30.06 CBV.dial SSV @14:51:31.57 CBV.dial between CSV and waste2b @14:51:32.74 CSV.dial between air and CTV @14:51:33.49 CS.seek 7.168ml @14:51:41.87 CTV.dial CSV,avoiding: :air @14:51:41.90 CSV.dial CTV @14:51:43.56 CS.reconfigure CSpressurize @14:51:43.66 CS.seek 0ml @14:52:02.06 delay 2:00 @14:53:09.88 delay 30 seconds @14:53:39.91 ATV.dial :waste,via: :air3 @14:53:41.72 AS.reconfigure ASconfig @14:53:41.81 AS.seek :empty @14:53:58.25 Parking Bleach in LysateSource line and SPEcolumn @14:53:58.26 ATV.dial :air3 @14:53:59.18 AS.reconfigure ASslow1 @14:53:59.26 AS.seek 0.029ml @14:54:02.09 CBV.dial :waste2b @14:54:02.50 CS.reconfigure CSconfig @14:54:02.60 CS.seek :empty @14:54:04.42 delay 1:00 @14:54:05.61 ATV.dial :ElutionWater @14:54:06.27 AS.seek 0.059ml @14:54:12.61 ATV.dial :air3 @14:54:13.29 AS.seek 0.069ml @14:54:15.59 delay 2 seconds @14:54:17.61 ATV.dial :bleach @14:54:18.12 AS.seek 0.769ml @14:55:04.45 CTV.dial :air,avoiding: :intake @14:55:06.11 Storing wcr puck in Collection Clamp to tube 1 @14:55:06.35 SC.reconfigure SCtight @14:55:06.44 SC.seek 1 @14:55:08.60 SC.reconfigure SCloose @14:55:08.86 Elbow.reconfigure TightElbow @14:55:08.95 Elbow.seek 1 @14:55:10.92 Forearm.reconfigure TightForearm @14:55:11.02 Forearm.seek 1 + 700 counts @14:55:12.11 SE.reconfigure SEpick @14:55:12.23 SE.seek beamBreak @14:55:15.43 SE.configure SEconfig @14:55:15.63 Elbow.reconfigure LimpElbow @14:55:15.73 Forearm.reconfigure LooseForearm @14:55:15.82 Forearm.seek 1 @14:55:16.18 Elbow.hold @14:55:16.30 Elbow.reconfigure TightElbow @14:55:16.97 SE.seek 1.07 Pucks below Top Plate @14:55:17.55 Forearm.force -20 @14:55:17.64 Forearm.seek 1 + 334 counts @14:55:18.00 Forearm.reconfigure TightForearm @14:55:18.09 Forearm.seek 1 + 700 counts @14:55:18.37 SE.seek 2.07 Pucks below Top Plate @14:55:19.01 Forearm.reconfigure LooseForearm @14:55:19.10 Forearm.seek :retract @14:55:20.07 Elbow.seek CC @14:55:21.75 Elbow.reconfigure LooseElbow @14:55:21.89 Forearm.seek CC @14:55:23.03 Hand.close @14:55:23.76 CC.seek :open @14:55:28.34 Forearm.seek :retract @14:55:29.42 Elbow.reconfigure TightElbow @14:55:29.51 Elbow.seek 1 @14:55:31.56 Elbow.reconfigure LimpElbow @14:55:31.65 Forearm.seek 1 @14:55:32.73 Elbow.hold @14:55:32.85 Elbow.reconfigure TightElbow @14:55:32.93 Hand.open @14:55:33.89 Forearm.force -20 @14:55:33.98 Forearm.seek 1 + 345 counts @14:55:34.34 Forearm.reconfigure TightForearm @14:55:34.43 Forearm.seek 1 + 700 counts @14:55:34.89 SE.seek :down @14:55:36.04 Forearm.reconfigure LooseForearm @14:55:36.13 Forearm.seek :retract @14:55:37.62 SE.coast @14:55:37.69 SC.coast @14:55:37.77 Starting finalPurge @14:55:37.79 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @14:55:37.92 Hand.open @14:55:38.40 Elbow.seek FlushPuck::Garage @14:55:40.50 Elbow.reconfigure LooseElbow @14:55:40.64 Forearm.seek :garage @14:55:41.73 Hand.close @14:55:42.64 Forearm.seek :retract @14:55:43.72 Elbow.reconfigure TightElbow @14:55:43.81 Elbow.seek CC @14:55:46.71 Elbow.reconfigure LooseElbow @14:55:46.86 Forearm.reconfigure TightForearm @14:55:47.56 Forearm.seek CC @14:55:48.98 CC.seek :closed @14:55:53.38 Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.41 @14:55:53.51 Hand.open @14:55:54.33 Forearm.reconfigure LooseForearm @14:55:54.42 Forearm.seek :retract @14:55:55.47 Flushing the Collection Valve::Manifold @14:55:55.65 CSV.dial CSR @14:55:56.24 Pulling 5ml of CSR.flush @14:55:56.25 CSR.select :flush @14:55:56.44 CS.seek 5ml @14:56:10.48 delay 5 seconds @14:56:15.51 CSR.select :air @14:56:15.71 CS.reconfigure CSfast @14:56:15.81 CS.seek 7.5ml @14:56:23.55 CSV.dial :waste @14:56:25.22 CS.reconfigure CSconfig @14:56:25.31 CS.seek 0ml @14:56:39.17 delay 3 seconds @14:56:42.20 ATV.dial :air3 @14:56:42.80 delay 3 seconds @14:56:45.74
Flushing lower manifold's waste1 port @14:56:45.97 CSV.dial :flush @14:56:46.01 AS.seek 0.794ml @14:56:46.65
CS.reconfigure CSfast @14:56:46.74 CS.seek 5ml @14:56:51.39 delay 3 seconds @14:56:54.42 ATV.dial :bleach @14:56:55.09 AS.seek 0.779ml @14:56:58.44 delay 3 seconds @14:57:00.67
delay 5 seconds @14:57:01.46 ATV.dial :AIV @14:57:02.80 AS.reconfigure ASconfig @14:57:02.89 AS.seek 0.015ml @14:57:05.69
CSV.dial :air @14:57:06.29 CS.seek 7ml @14:57:12.45 CSV.dial CTV @14:57:12.49
CTV.dial CSV @14:57:14.14 CBV.dial :waste1 @14:57:15.09 CS.seek 0ml @14:57:33.95 Flushing lower manifold's waste2b port @14:57:34.13 CSV.dial :flush @14:57:35.02 CS.seek 5ml @14:57:47.13 ATV.dial :waste @14:57:48.73 AS.seek :empty @14:57:49.11
delay 5 seconds @14:57:49.97 ATV.dial :air3 @14:57:50.70 AS.reconfigure ASslow1 @14:57:50.78 AS.seek 0.009ml @14:57:53.17 delay 2 seconds @14:57:54.13
CSV.dial :air @14:57:54.76 CS.seek 7ml @14:57:55.19 ATV.dial :bleach @14:57:55.68 AS.seek 0.579ml @14:58:00.98 CSV.dial CTV @14:58:01.02
CTV.dial CSV @14:58:01.69 CBV.dial :waste2b @14:58:02.62 CS.seek 0ml @14:58:21.73 Flushing upper manifold @14:58:22.46 CSV.dial :flush @14:58:23.35 CS.seek 5ml @14:58:37.23 delay 5 seconds @14:58:42.25 CSV.dial :air @14:58:42.90 CS.seek 7ml @14:58:49.08 CSV.dial CBV @14:58:49.12
CBV.dial CSV @14:58:50.56 CTV.dial :air @14:58:52.16 CS.seek 0ml @14:59:11.33 delay 7 seconds @14:59:18.37 CS.seek 3ml @14:59:27.04 CSV.dial :waste @14:59:27.58 CS.seek 0ml @14:59:36.81 CSV.dial :flush @14:59:37.39 CS.seek 3ml @14:59:46.23 delay 5 seconds @14:59:50.63 delay 3 seconds @14:59:51.25
CSV.dial :air @14:59:51.84 CS.seek 5ml @14:59:53.66 ATV.dial :air3 @14:59:54.27 delay 3 seconds @14:59:57.36 AS.seek 0.604ml @14:59:58.08
CSV.dial :air @14:59:58.11 CS.seek 0ml @15:00:02.72 delay 3 seconds @15:00:05.75 ATV.dial :bleach @15:00:06.41 AS.seek 0.589ml @15:00:09.75 delay 3 seconds @15:00:12.02
delay 7 seconds @15:00:12.77 ATV.dial :ASV @15:00:13.88 ASV.dial :wasteR @15:00:14.51 AS.seek 0ml @15:00:19.06
CS.seek 3ml @15:00:27.89 CSV.dial :waste @15:00:28.79 CS.seek 0ml @15:00:37.61 Disinfecting external Intake with mfbkill @15:00:37.79 CSV.dial CSR @15:00:39.51 Pulling 8ml of CSR.mfbkill @15:00:39.53 CSR.select :mfbkill @15:00:39.71 CS.seek 8ml @15:01:01.30 delay 5 seconds @15:01:06.33 CSR.select :air @15:01:06.50 CS.seek 10ml @15:01:12.74 CSV.dial CBV @15:01:12.78
CBV.dial CSV @15:01:14.13 CTV.dial :intake @15:01:15.03 Intake.select :open @15:01:15.19 CS.seek 2ml @15:01:38.08 Intake.select :close @15:01:38.23 CTV.dial :air @15:01:39.14 CS.reconfigure CSconfig @15:01:39.23 CS.seek 0ml @15:01:45.39 Flushing lower manifold's waste1 port @15:01:45.41 CSV.dial CSR @15:01:46.70 Pulling 4ml of CSR.mfbkill @15:01:46.72 CSR.select :mfbkill @15:01:46.90 CS.seek 4ml @15:01:58.33 delay 5 seconds @15:02:03.36 CSR.select :air @15:02:03.52 CS.reconfigure CSfast @15:02:03.61 CS.seek 10ml @15:02:13.31 delay 1:00 @15:02:20.14 CSV.dial CTV @15:02:20.18
CTV.dial CSV @15:02:21.86 CBV.dial :waste1 @15:02:22.43 CS.seek 0ml @15:02:49.24 Flushing lower manifold's waste2b port @15:02:49.26 CSV.dial CSR @15:02:49.90 Pulling 4ml of CSR.mfbkill @15:02:49.92 CSR.select :mfbkill @15:02:50.10 CS.reconfigure CSconfig @15:02:50.18 CS.seek 4ml @15:03:01.49 delay 5 seconds @15:03:06.52 CSR.select :air @15:03:06.68 CS.reconfigure CSfast @15:03:06.77 CS.seek 10ml @15:03:13.34 AFV.select :carrierSolution @15:03:13.48 delay 2 seconds @15:03:15.51 AS.reconfigure ASconfig @15:03:15.59 AS.seek 1ml @15:03:23.80 CSV.dial CTV @15:03:23.84
CTV.dial CSV @15:03:24.42 CBV.dial :waste2b @15:03:25.36 CS.seek 0ml @15:03:52.21 Killing upper manifold @15:03:52.23 CSV.dial CSR @15:03:52.87 Pulling 4ml of CSR.mfbkill @15:03:52.88 CSR.select :mfbkill @15:03:53.07 CS.reconfigure CSconfig @15:03:53.15 CS.seek 4ml @15:04:04.46 delay 5 seconds @15:04:09.49 CSR.select :air @15:04:09.64 CS.reconfigure CSfast @15:04:09.73 CS.seek 10ml @15:04:13.38 delay 1 second @15:04:14.40 AFV.select :holdingCoil @15:04:14.53 delay 1 second @15:04:15.57 ATV.dial ASV @15:04:15.58 ASV.dial :wasteR @15:04:15.64 AS.reconfigure ASslow1 @15:04:15.73 AS.seek 0ml @15:04:26.30 CSV.dial CBV @15:04:26.34
CBV.dial CSV @15:04:27.61 CTV.dial :air @15:04:29.20 CS.seek 0ml @15:04:55.80 delay 7 seconds @15:05:02.84 CS.seek 3ml @15:05:11.61 CSV.dial :waste @15:05:12.17 CS.seek 0ml @15:05:21.11 Flushing upper manifold @15:05:21.29 CSV.dial :flush @15:05:21.82 CS.seek 5ml @15:05:35.67 delay 5 seconds @15:05:40.70 CSV.dial :air @15:05:41.28 CS.seek 7ml @15:05:47.46 CSV.dial CBV @15:05:47.50
CBV.dial CSV @15:05:48.75 CTV.dial :air @15:05:48.80 CS.seek 0ml @15:06:07.74 delay 7 seconds @15:06:14.78 CS.seek 3ml @15:06:23.55 CSV.dial :waste @15:06:24.12 CS.seek 0ml @15:06:32.89 CSV.dial :flush @15:06:33.45 CS.seek 3ml @15:06:42.30 delay 5 seconds @15:06:47.33 CSV.dial :air @15:06:47.93 CS.seek 5ml @15:06:54.14 CSV.dial :air @15:06:54.17 CS.seek 0ml @15:07:07.96 delay 7 seconds @15:07:15.00 CS.seek 3ml @15:07:23.77 CSV.dial :waste @15:07:24.67 CS.seek 0ml @15:07:33.30 Flushing lower manifold's waste1 port @15:07:33.48 CSV.dial :flush @15:07:34.03 CS.seek 5ml @15:07:37.16 delay 20 seconds @15:07:47.89
delay 5 seconds @15:07:52.92 CSV.dial :air @15:07:53.54 CS.seek 7ml @15:07:57.18 ATV.dial :air3 @15:07:58.78 AS.seek 0.59ml @15:07:59.74 CSV.dial CTV @15:07:59.78
CTV.dial CSV @15:08:01.44 CBV.dial :waste1 @15:08:02.03 CS.seek 0ml @15:08:20.99 Flushing lower manifold's waste2b port @15:08:21.17 CSV.dial :flush @15:08:22.08 CS.seek 5ml @15:08:36.52 delay 5 seconds @15:08:41.55 CSV.dial :air @15:08:42.18 CS.seek 7ml @15:08:48.43 CSV.dial CTV @15:08:48.47
CTV.dial CSV @15:08:49.00 CBV.dial :waste2b @15:08:49.95 CS.seek 0ml @15:09:08.93 Disinfecting external Intake with kill @15:09:09.11 CSV.dial CSR @15:09:09.71 Pulling 8ml of CSR.kill @15:09:09.72 CSR.select :kill @15:09:09.91 CS.seek 8ml @15:09:31.55 delay 5 seconds @15:09:36.58 CSR.select :air @15:09:36.75 CS.seek 10ml @15:09:42.93 CSV.dial CBV @15:09:42.97
CBV.dial CSV @15:09:44.35 CTV.dial :intake @15:09:45.24 Intake.select :open @15:09:45.40 CS.seek 2ml @15:09:57.80 delay 3 seconds @15:10:00.83 ATV.dial :ASV @15:10:02.34 ASV.dial :wasteR @15:10:02.46 AS.seek 0.09ml @15:10:08.49
Intake.select :close @15:10:08.64 CTV.dial :air @15:10:09.54 CS.reconfigure CSconfig @15:10:09.64 CS.seek 0ml @15:10:15.86 CSV.dial CSR @15:10:17.14 Pulling 5ml of CSR.flush @15:10:17.16 CSR.select :flush @15:10:17.34 CS.seek 5ml @15:10:31.36 delay 5 seconds @15:10:36.39 CSR.select :air @15:10:36.56 CS.seek 10ml @15:10:50.48 CSV.dial CBV @15:10:51.75 CS.seek 0ml @15:11:18.40 Garaging FlushPuck in Collection Clamp @15:11:18.52 Elbow.reconfigure TightElbow @15:11:18.61 Elbow.seek CC @15:11:18.85 Elbow.reconfigure LooseElbow @15:11:19.00 Forearm.seek CC @15:11:20.09 Hand.close @15:11:20.90 CC.seek :open @15:11:25.34 Forearm.seek :retract @15:11:26.42 Elbow.reconfigure TightElbow @15:11:26.51 Elbow.seek :garage @15:11:29.67 Elbow.reconfigure LooseElbow @15:11:29.82 Forearm.reconfigure TightForearm @15:11:29.91 Forearm.seek :garage @15:11:31.12 Hand.open @15:11:31.97 Forearm.reconfigure LooseForearm @15:11:32.05 Forearm.seek :retract @15:11:33.15 Starting initialPurge @15:11:33.17 Loading FlushPuck into Collection Clamp from FlushPuck::Garage @15:11:33.30 Hand.open @15:11:33.60 Elbow.reconfigure TightElbow @15:11:33.68 Elbow.seek FlushPuck::Garage @15:11:33.83 Elbow.reconfigure LooseElbow @15:11:33.98 Forearm.seek :garage @15:11:35.08 Hand.close @15:11:36.03 Forearm.seek :retract @15:11:37.14 Elbow.reconfigure TightElbow @15:11:37.22 Elbow.seek CC @15:11:40.07 Elbow.reconfigure LooseElbow @15:11:40.75 Forearm.reconfigure TightForearm @15:11:40.84 Forearm.seek CC @15:11:42.55 CC.seek :closed @15:11:43.39 delay 30 seconds @15:11:47.02
Collection Clamp onSpring/offSpring for 7/31 samples, on/off torque 2.35 @15:11:47.13 Hand.open @15:11:47.97 Forearm.reconfigure LooseForearm @15:11:48.05 Forearm.seek :retract @15:11:49.14 Flushing the Collection Valve::Manifold @15:11:49.32 CSV.dial CSR @15:11:50.72 Pulling 5ml of CSR.flush @15:11:50.74 CSR.select :flush @15:11:50.92 CS.seek 5ml @15:12:04.90 delay 5 seconds @15:12:09.93 CSR.select :air @15:12:10.14 CS.reconfigure CSfast @15:12:10.23 CS.seek 7.501ml @15:12:13.43 ATV.dial :extSrc @15:12:14.25 delay 3 seconds @15:12:17.28 ATV.dial :ElutionWater @15:12:17.64
CSV.dial :waste @15:12:18.46 AS.seek 0.12ml @15:12:19.19
CS.reconfigure CSconfig @15:12:19.28 CS.seek 0ml @15:12:24.83 ATV.dial :air3 @15:12:25.49 AS.seek 0.13ml @15:12:27.83 delay 2 seconds @15:12:29.85 ATV.dial :bleach @15:12:30.37 AS.seek 0.23ml @15:12:39.46
Purging external Intake @15:12:39.63 CSV.dial :flush @15:12:40.18 CS.reconfigure CSfast @15:12:40.27 CS.seek 10ml @15:12:50.77 delay 3 seconds @15:12:53.80 ATV.dial :air3 @15:12:54.39 delay 3 seconds @15:12:57.48 AS.seek 0.255ml @15:13:02.84 delay 3 seconds @15:13:05.87 ATV.dial :bleach @15:13:06.54 AS.seek 0.24ml @15:13:07.21
delay 5 seconds @15:13:09.81 delay 3 seconds @15:13:12.24 CSV.dial CBV @15:13:12.28
CBV.dial CSV @15:13:12.83 ATV.dial :ASV @15:13:13.18
CTV.dial :intake @15:13:13.92 ASV.dial :SPEdelivery @15:13:14.10
Intake.select :open @15:13:14.25 CS.seek 0ml @15:13:14.36 AFV.select :carrierSolution @15:13:14.50 delay 2 seconds @15:13:16.53 AS.reconfigure ASconfig @15:13:16.61 AS.seek 0.5ml @15:13:31.87 delay 1 second @15:13:32.89 AFV.select :holdingCoil @15:13:33.07 AS.reconfigure ASslow1 @15:13:33.16 AS.seek 0.17ml @15:13:42.67
Intake.select :close @15:13:42.81 CTV.dial :air @15:13:43.73 Flushing upper manifold @15:13:43.90 CSV.dial :flush @15:13:44.80 CS.seek 5ml @15:13:58.68 delay 5 seconds @15:14:03.71 CSV.dial :air @15:14:04.33 CS.seek 7ml @15:14:10.55 CSV.dial CBV @15:14:10.58
CBV.dial CSV @15:14:11.84 CTV.dial :air @15:14:11.87 CS.seek 0ml @15:14:30.84 delay 7 seconds @15:14:37.88 CS.seek 3ml @15:14:39.87 SPE.hold 2:46:40,95C @15:14:40.53 delay 3:00 @15:14:46.92
CSV.dial :waste @15:14:47.52 CS.seek 0ml @15:14:56.71 CSV.dial :flush @15:14:57.28 CS.seek 3ml @15:15:06.34 delay 5 seconds @15:15:11.37 CSV.dial :air @15:15:12.00 CS.seek 5ml @15:15:18.43 CSV.dial :air @15:15:18.46 CS.seek 0ml @15:15:32.93 delay 7 seconds @15:15:39.97 CS.seek 3ml @15:15:49.15 CSV.dial :waste @15:15:50.10 CS.seek 0ml @15:15:59.12 Garaging FlushPuck in Collection Clamp @15:15:59.24 Elbow.reconfigure TightElbow @15:15:59.32 Elbow.seek CC @15:15:59.58 Elbow.reconfigure LooseElbow @15:15:59.73 Forearm.seek CC @15:16:00.82 Hand.close @15:16:01.53 CC.seek :open @15:16:05.97 Forearm.seek :retract @15:16:07.08 Elbow.reconfigure TightElbow @15:16:07.17 Elbow.seek :garage @15:16:10.42 Elbow.reconfigure LooseElbow @15:16:10.56 Forearm.reconfigure TightForearm @15:16:10.64 Forearm.seek :garage @15:16:11.88 Hand.open @15:16:12.73 Forearm.reconfigure LooseForearm @15:16:12.81 Forearm.seek :retract @15:16:13.93 Completed [coreNEG] begun 13:40:30 @15:16:13.95 Extending InvasivePostCoreNeg until completion of MFBPCR @15:17:40.56 AFV.select :carrierSolution @15:17:40.70 delay 2 seconds @15:17:42.73 AS.reconfigure ASconfig @15:17:42.81 AS.seek 1ml @15:18:30.84 delay 1 second @15:18:31.86 AFV.select :holdingCoil @15:18:31.99 delay 1 second @15:18:33.02 ASV.dial :wasteD @15:18:34.17 AS.seek 0ml @15:19:32.00 Priming Eluate @15:19:32.03 AFV.select :carrierSolution @15:19:32.27 delay 2 seconds @15:19:34.35 AS.seek 0.5ml @15:20:03.55 delay 1 second @15:20:04.57 AFV.select :holdingCoil @15:20:04.70 ATV.dial ASV @15:20:04.72 ASV.dial :SPErecovery @15:20:05.39 Swish #1 of 2 @15:20:05.50 AS.reconfigure ASslow0 @15:20:05.58 AS.seek 0.6ml @15:20:16.07 AS.seek 0.5ml @15:20:26.49 Swish #2 of 2 @15:20:26.61 AS.seek 0.6ml @15:20:37.04 AS.seek 0.5ml @15:20:47.42 delay 3 seconds @15:20:50.51 AS.reconfigure ASslow1 @15:20:50.59 AS.seek 0.684ml @15:21:27.98 delay 3 seconds @15:21:31.01 Eluate primed @15:21:31.02 ATV.dial :waste2 @15:21:31.73 AS.reconfigure ASnorm @15:21:31.82 AS.seek 0ml @15:22:06.65 SPE.coast @15:22:06.76 ASV.dial :SPErecovery @15:22:06.80 Setting up PCR for OM -- writing /var/log/esp/OM20jul1415h.pcr @15:22:06.85 Building PCR Slug from ASV with MM3 mix, PP5 primer @15:22:06.89 Priming with carrier @15:22:06.93 ATV.dial ARV @15:22:07.95 ARV.dial :carrier @15:22:08.35 AS.seek 0.5ml @15:22:33.94 AFV.select :carrierSolution @15:22:34.10 delay 2 seconds @15:22:36.13 AS.reconfigure ASconfig @15:22:36.22 AS.seek 1ml @15:23:05.28 delay 1 second @15:23:06.30 AFV.select :holdingCoil @15:23:06.44 delay 1 second @15:23:07.47 ATV.dial :waste @15:23:09.00 AS.reconfigure ASnorm @15:23:09.09 AS.seek :empty @15:23:59.83 AFV.select :carrierSolution @15:23:59.99 delay 2 seconds @15:24:02.02 AS.reconfigure ASconfig @15:24:02.11 AS.seek 0.5ml @15:24:31.76 delay 1 second @15:24:32.78 AFV.select :holdingCoil @15:24:32.91 Building Slug @15:24:32.92 ATV.dial :ARV @15:24:34.62 delay 2 seconds @15:24:36.65 ARV.dial :air1 @15:24:37.09 delay 2 seconds @15:24:39.18 AS.reconfigure ASslow2 @15:24:39.27 AS.seek 0.506ml @15:24:45.61 delay 2 seconds @15:24:47.64 ARV.dial :MM3 @15:24:48.75 delay 2 seconds @15:24:50.78 Adding MM3 @15:24:50.91 AS.seek 0.524ml @15:25:09.31 delay 25 seconds @15:25:34.34 ARV.dial :PP5 @15:25:34.78 Adding PP5 @15:25:34.80 delay 2 seconds @15:25:36.91 AS.seek 0.53ml @15:25:43.31 delay 10 seconds @15:25:53.34 ARV.dial :air3 @15:25:53.77 adding ARV air @15:25:53.79 delay 2 seconds @15:25:55.88 AS.seek 0.536ml @15:26:02.09 delay 5 seconds @15:26:07.12 ARV.dial :carrier @15:26:08.45 delay 7 seconds @15:26:15.54 AS.seek 0.594ml @15:27:14.06 delay 5 seconds @15:27:19.09 ATV.dial :ASV @15:27:19.53 delay 2 seconds @15:27:21.62 AS.seek 0.6ml @15:27:27.98 delay 4 seconds @15:27:32.01 ATV.dial ARV @15:27:32.59 delay 2 seconds @15:27:34.62 ARV.dial :carrier @15:27:34.64 delay 2 seconds @15:27:36.74 AS.seek 0.65ml @15:28:27.42 delay 4 seconds @15:28:31.45 ATV.dial PCR @15:28:32.11 ASV.dial :waste1 @15:28:33.01 ARV.dial :air1 @15:28:33.45 Positioning Slug by 0.324ml @15:28:33.58 AS.seek 0.326ml @15:33:59.72 delay 5 seconds @15:34:04.75 ATV.dial ARV @15:34:05.58 ASV.dial :PCRlock @15:34:06.00 delay 5 seconds @15:34:11.03 Running OM Profile60C350 thermocycling @15:34:11.05 PCR.program Profile60C350 @15:34:11.22 Profile60C350 should complete in < 2:02:45 @15:34:11.24 PCR.run 2:38:26.25 @17:33:10.05 PCR.off @17:33:11.37 PCR for OM completed -- closed /var/log/esp/OM20jul1415h.pcr @17:33:11.47 Cleaning PCR @17:33:11.49 AFV.select :carrierSolution @17:33:11.64 delay 2 seconds @17:33:13.67 AS.reconfigure ASconfig @17:33:13.76 AS.seek 0.5ml @17:33:24.12 delay 1 second @17:33:25.14 AFV.select :holdingCoil @17:33:25.27 ASV.dial :waste1 @17:33:25.77 ATV.dial :air3 @17:33:27.12 AS.reconfigure ASslow1 @17:33:27.20 AS.seek 0.51ml @17:33:29.51 delay 2 seconds @17:33:31.53 ATV.dial :bleach @17:33:32.20 AS.seek 0.81ml @17:34:32.84 delay 3 seconds @17:34:35.87 ATV.dial :air3 @17:34:36.44 delay 3 seconds @17:34:39.53 AS.seek 0.835ml @17:34:44.88 delay 3 seconds @17:34:47.91 ATV.dial :bleach @17:34:48.56 AS.seek 0.82ml @17:34:51.88 delay 3 seconds @17:34:54.91 ARV.dial :waste @17:34:55.55 delay 3 seconds @17:34:58.58 ATV.dial :ARV @17:34:59.50 AS.reconfigure ASconfig @17:34:59.59 AS.seek 0.47ml @17:35:20.03 AFV.select :carrierSolution @17:35:20.18 delay 2 seconds @17:35:22.21 AS.seek 1ml @17:35:53.04 delay 1 second @17:35:54.06 AFV.select :holdingCoil @17:35:54.19 delay 1 second @17:35:55.22 AS.reconfigure ASfast @17:35:55.30 AS.seek 0ml @17:36:22.65 AFV.select :carrierSolution @17:36:22.79 delay 2 seconds @17:36:24.82 AS.reconfigure ASconfig @17:36:24.90 AS.seek 1ml @17:37:22.72 delay 1 second @17:37:23.74 AFV.select :holdingCoil @17:37:23.87 delay 1 second @17:37:24.90 AS.reconfigure ASfast @17:37:24.98 AS.seek 0ml @17:37:52.37 AFV.select :carrierSolution @17:37:52.51 delay 2 seconds @17:37:54.54 AS.reconfigure ASconfig @17:37:54.62 AS.seek 1ml @17:38:52.44 delay 1 second @17:38:53.46 AFV.select :holdingCoil @17:38:53.59 delay 1 second @17:38:54.62 AS.reconfigure ASfast @17:38:54.70 AS.seek 0ml @17:39:22.09 ARV.dial between carrier and air1 @17:39:22.62 ATV.dial :air3 @17:39:23.95 AS.reconfigure ASslow1 @17:39:24.05 AS.seek 0.01ml @17:39:26.51 delay 2 seconds @17:39:28.53 ATV.dial :bleach @17:39:29.19 AS.seek 0.81ml @17:42:10.44 delay 3 seconds @17:42:13.47 ATV.dial :air3 @17:42:14.05 delay 3 seconds @17:42:17.14 AS.seek 0.835ml @17:42:22.50 delay 3 seconds @17:42:25.53 ATV.dial :bleach @17:42:26.19 AS.seek 0.82ml @17:42:29.51 delay 3 seconds @17:42:32.54 ATV.dial PCR @17:42:32.97 AS.reconfigure ASconfig @17:42:33.05 AS.seek :empty @17:43:20.54 PCR.program :bleachProfile @17:43:20.62 PCR program should complete in < 2:45 @17:43:20.64 PCR.run 10:00 @17:46:10.95 PCR.off @17:46:11.01 AFV.select :carrierSolution @17:46:11.14 delay 2 seconds @17:46:13.18 AS.seek 1ml @17:47:11.04 delay 1 second @17:47:12.06 AFV.select :holdingCoil @17:47:12.19 delay 1 second @17:47:13.22 AS.reconfigure ASfast @17:47:13.30 AS.seek 0ml @17:47:40.68 delay 3 seconds @17:47:43.71 AFV.select :carrierSolution @17:47:43.84 delay 2 seconds @17:47:45.87 AS.reconfigure ASconfig @17:47:45.96 AS.seek 1ml @17:48:43.79 delay 1 second @17:48:44.81 AFV.select :holdingCoil @17:48:44.94 delay 1 second @17:48:45.97 AS.reconfigure ASfast @17:48:46.05 AS.seek 0ml @17:49:13.44 delay 3 seconds @17:49:16.47 AFV.select :carrierSolution @17:49:16.61 delay 2 seconds @17:49:18.64 AS.reconfigure ASconfig @17:49:18.72 AS.seek 1ml @17:50:16.54 delay 1 second @17:50:17.56 AFV.select :holdingCoil @17:50:17.69 delay 1 second @17:50:18.72 AS.reconfigure ASfast @17:50:18.80 AS.seek 0ml @17:50:46.19 delay 3 seconds @17:50:49.22 AFV.select :carrierSolution @17:50:49.35 delay 2 seconds @17:50:51.38 AS.reconfigure ASconfig @17:50:51.47 AS.seek 1ml @17:51:49.24 delay 1 second @17:51:50.27 AFV.select :holdingCoil @17:51:50.40 delay 1 second @17:51:51.43 AS.reconfigure ASfast @17:51:51.51 AS.seek 0ml @17:52:18.90 delay 3 seconds @17:52:21.93 AFV.select :carrierSolution @17:52:22.07 delay 2 seconds @17:52:24.10 AS.reconfigure ASconfig @17:52:24.19 AS.seek 1ml @17:53:21.97 delay 1 second @17:53:22.99 AFV.select :holdingCoil @17:53:23.12 delay 1 second @17:53:24.15 AS.reconfigure ASnorm @17:53:24.23 AS.seek 0ml @17:54:14.98 Setting up PCR for ML -- writing /var/log/esp/ML20jul1417h.pcr @17:54:15.00 Building PCR Slug from ASV with MM2 mix, PP4 primer @17:54:15.02 Priming with carrier @17:54:15.08 ATV.dial ARV @17:54:15.75 ARV.dial :carrier @17:54:16.14 AS.seek 0.5ml @17:54:41.76 AFV.select :carrierSolution @17:54:41.91 delay 2 seconds @17:54:43.94 AS.reconfigure ASconfig @17:54:44.03 AS.seek 1ml @17:55:13.08 delay 1 second @17:55:14.10 AFV.select :holdingCoil @17:55:14.24 delay 1 second @17:55:15.27 ATV.dial :waste @17:55:16.96 AS.reconfigure ASnorm @17:55:17.06 AS.seek :empty @17:56:07.81 AFV.select :carrierSolution @17:56:07.97 delay 2 seconds @17:56:10.00 AS.reconfigure ASconfig @17:56:10.09 AS.seek 0.5ml @17:56:39.24 delay 1 second @17:56:40.26 AFV.select :holdingCoil @17:56:40.39 Building Slug @17:56:40.40 ATV.dial :ARV @17:56:42.11 delay 2 seconds @17:56:44.14 ARV.dial :air1 @17:56:44.55 delay 2 seconds @17:56:46.63 AS.reconfigure ASslow2 @17:56:46.72 AS.seek 0.506ml @17:56:52.96 delay 2 seconds @17:56:54.98 ARV.dial :MM2 @17:56:56.49 delay 2 seconds @17:56:58.52 Adding MM2 @17:56:58.63 AS.seek 0.524ml @17:57:17.04 delay 25 seconds @17:57:42.07 ARV.dial :PP4 @17:57:42.50 Adding PP4 @17:57:42.52 delay 2 seconds @17:57:44.62 AS.seek 0.53ml @17:57:51.06 delay 10 seconds @17:58:01.09 ARV.dial :air3 @17:58:01.52 adding ARV air @17:58:01.54 delay 2 seconds @17:58:03.64 AS.seek 0.536ml @17:58:09.90 delay 5 seconds @17:58:14.93 ARV.dial :carrier @17:58:16.24 delay 7 seconds @17:58:23.33 AS.seek 0.594ml @17:59:22.63 delay 5 seconds @17:59:27.66 ATV.dial :ASV @17:59:28.11 delay 2 seconds @17:59:30.20 AS.seek 0.6ml @17:59:36.44 delay 4 seconds @17:59:40.47 ATV.dial ARV @17:59:41.07 delay 2 seconds @17:59:43.10 ARV.dial :carrier @17:59:43.12 delay 2 seconds @17:59:45.21 AS.seek 0.65ml @18:00:35.79 delay 4 seconds @18:00:39.82 ATV.dial PCR @18:00:40.47 ASV.dial :waste1 @18:00:40.49 ARV.dial :air1 @18:00:40.95 Positioning Slug by 0.324ml @18:00:41.07 AS.seek 0.326ml @18:06:06.94 delay 5 seconds @18:06:11.97 ATV.dial ARV @18:06:12.79 ASV.dial :PCRlock @18:06:13.21 delay 5 seconds @18:06:18.24 Running ML Profile60C350 thermocycling @18:06:18.25 PCR.program Profile60C350 @18:06:18.41 Profile60C350 should complete in < 2:02:45 @18:06:18.43 PCR.run 2:38:26.25 @20:05:24.26 PCR.off @20:05:25.60 PCR for ML completed -- closed /var/log/esp/ML20jul1417h.pcr @20:05:25.71 Cleaning PCR @20:05:25.73 AFV.select :carrierSolution @20:05:25.88 delay 2 seconds @20:05:27.96 AS.reconfigure ASconfig @20:05:28.04 AS.seek 0.5ml @20:05:38.39 delay 1 second @20:05:39.41 AFV.select :holdingCoil @20:05:39.54 ASV.dial :waste1 @20:05:39.95 ATV.dial :air3 @20:05:41.29 AS.reconfigure ASslow1 @20:05:41.38 AS.seek 0.51ml @20:05:43.71 delay 2 seconds @20:05:45.73 ATV.dial :bleach @20:05:46.39 AS.seek 0.81ml @20:06:47.01 delay 3 seconds @20:06:50.04 ATV.dial :air3 @20:06:50.62 delay 3 seconds @20:06:53.71 AS.seek 0.835ml @20:06:58.96 delay 3 seconds @20:07:01.99 ATV.dial :bleach @20:07:02.64 AS.seek 0.82ml @20:07:05.98 delay 3 seconds @20:07:09.00 ARV.dial :waste @20:07:09.64 delay 3 seconds @20:07:12.67 ATV.dial :ARV @20:07:14.29 AS.reconfigure ASconfig @20:07:14.38 AS.seek 0.47ml @20:07:34.80 AFV.select :carrierSolution @20:07:34.94 delay 2 seconds @20:07:36.98 AS.seek 1ml @20:08:07.78 delay 1 second @20:08:08.80 AFV.select :holdingCoil @20:08:08.92 delay 1 second @20:08:09.95 AS.reconfigure ASfast @20:08:10.03 AS.seek 0ml @20:08:37.39 AFV.select :carrierSolution @20:08:37.53 delay 2 seconds @20:08:39.56 AS.reconfigure ASconfig @20:08:39.65 AS.seek 1ml @20:09:37.45 delay 1 second @20:09:38.47 AFV.select :holdingCoil @20:09:38.59 delay 1 second @20:09:39.62 AS.reconfigure ASfast @20:09:39.71 AS.seek 0ml @20:10:07.10 AFV.select :carrierSolution @20:10:07.24 delay 2 seconds @20:10:09.27 AS.reconfigure ASconfig @20:10:09.35 AS.seek 1ml @20:11:07.14 delay 1 second @20:11:08.16 AFV.select :holdingCoil @20:11:08.29 delay 1 second @20:11:09.32 AS.reconfigure ASfast @20:11:09.40 AS.seek 0ml @20:11:36.79 ARV.dial between carrier and air1 @20:11:37.34 ATV.dial :air3 @20:11:38.67 AS.reconfigure ASslow1 @20:11:38.76 AS.seek 0.01ml @20:11:41.15 delay 2 seconds @20:11:43.17 ATV.dial :bleach @20:11:43.83 AS.seek 0.81ml @20:14:25.00 delay 3 seconds @20:14:28.03 ATV.dial :air3 @20:14:28.62 delay 3 seconds @20:14:31.71 AS.seek 0.835ml @20:14:36.97 delay 3 seconds @20:14:40.00 ATV.dial :bleach @20:14:40.66 AS.seek 0.82ml @20:14:44.00 delay 3 seconds @20:14:47.03 ATV.dial PCR @20:14:47.45 AS.reconfigure ASconfig @20:14:47.53 AS.seek :empty @20:15:35.01 PCR.program :bleachProfile @20:15:35.09 PCR program should complete in < 2:45 @20:15:35.10 PCR.run 10:00 @20:18:24.86 PCR.off @20:18:24.90 AFV.select :carrierSolution @20:18:25.05 delay 2 seconds @20:18:27.08 AS.seek 1ml @20:19:24.95 delay 1 second @20:19:25.97 AFV.select :holdingCoil @20:19:26.09 delay 1 second @20:19:27.12 AS.reconfigure ASfast @20:19:27.21 AS.seek 0ml @20:19:54.59 delay 3 seconds @20:19:57.62 AFV.select :carrierSolution @20:19:57.76 delay 2 seconds @20:19:59.79 AS.reconfigure ASconfig @20:19:59.89 AS.seek 1ml @20:20:57.71 delay 1 second @20:20:58.73 AFV.select :holdingCoil @20:20:58.86 delay 1 second @20:20:59.89 AS.reconfigure ASfast @20:20:59.98 AS.seek 0ml @20:21:27.35 delay 3 seconds @20:21:30.38 AFV.select :carrierSolution @20:21:30.50 delay 2 seconds @20:21:32.53 AS.reconfigure ASconfig @20:21:32.62 AS.seek 1ml @20:22:30.45 delay 1 second @20:22:31.47 AFV.select :holdingCoil @20:22:31.59 delay 1 second @20:22:32.62 AS.reconfigure ASfast @20:22:32.70 AS.seek 0ml @20:23:00.07 delay 3 seconds @20:23:03.10 AFV.select :carrierSolution @20:23:03.22 delay 2 seconds @20:23:05.25 AS.reconfigure ASconfig @20:23:05.35 AS.seek 1ml @20:24:03.17 delay 1 second @20:24:04.19 AFV.select :holdingCoil @20:24:04.32 delay 1 second @20:24:05.35 AS.reconfigure ASfast @20:24:05.44 AS.seek 0ml @20:24:32.81 delay 3 seconds @20:24:35.84 AFV.select :carrierSolution @20:24:35.98 delay 2 seconds @20:24:38.01 AS.reconfigure ASconfig @20:24:38.11 AS.seek 1ml @20:25:35.90 delay 1 second @20:25:36.92 AFV.select :holdingCoil @20:25:37.05 delay 1 second @20:25:38.08 AS.reconfigure ASnorm @20:25:38.17 AS.seek 0ml @20:26:28.95 Setting up PCR for CG -- writing /var/log/esp/CG20jul1420h.pcr @20:26:28.98 Building PCR Slug from ASV with MM1 mix, PP2 primer @20:26:29.00 Priming with carrier @20:26:29.04 ATV.dial ARV @20:26:30.31 ARV.dial :carrier @20:26:30.71 AS.seek 0.5ml @20:26:56.28 AFV.select :carrierSolution @20:26:56.44 delay 2 seconds @20:26:58.47 AS.reconfigure ASconfig @20:26:58.56 AS.seek 1ml @20:27:27.62 delay 1 second @20:27:28.64 AFV.select :holdingCoil @20:27:28.78 delay 1 second @20:27:29.81 ATV.dial :waste @20:27:31.53 AS.reconfigure ASnorm @20:27:31.62 AS.seek :empty @20:28:22.41 AFV.select :carrierSolution @20:28:22.57 delay 2 seconds @20:28:24.60 AS.reconfigure ASconfig @20:28:24.69 AS.seek 0.5ml @20:28:53.82 delay 1 second @20:28:54.84 AFV.select :holdingCoil @20:28:54.97 Building Slug @20:28:54.99 ATV.dial :ARV @20:28:56.68 delay 2 seconds @20:28:58.71 ARV.dial :air1 @20:28:59.13 delay 2 seconds @20:29:01.21 AS.reconfigure ASslow2 @20:29:01.30 AS.seek 0.506ml @20:29:07.54 delay 2 seconds @20:29:09.57 ARV.dial :MM1 @20:29:10.21 delay 2 seconds @20:29:12.24 Adding MM1 @20:29:12.36 AS.seek 0.524ml @20:29:30.81 delay 25 seconds @20:29:55.84 ARV.dial :PP2 @20:29:56.28 Adding PP2 @20:29:56.30 delay 2 seconds @20:29:58.41 AS.seek 0.53ml @20:30:04.67 delay 10 seconds @20:30:14.70 ARV.dial :air2 @20:30:15.12 adding ARV air @20:30:15.14 delay 2 seconds @20:30:17.23 AS.seek 0.536ml @20:30:23.56 delay 5 seconds @20:30:28.59 ARV.dial :carrier @20:30:29.93 delay 7 seconds @20:30:37.02 AS.seek 0.594ml @20:31:35.70 delay 5 seconds @20:31:40.73 ATV.dial :ASV @20:31:41.17 delay 2 seconds @20:31:43.26 AS.seek 0.6ml @20:31:49.53 delay 4 seconds @20:31:53.56 ATV.dial ARV @20:31:54.12 delay 2 seconds @20:31:56.15 ARV.dial :carrier @20:31:56.17 delay 2 seconds @20:31:58.26 AS.seek 0.65ml @20:32:48.81 delay 4 seconds @20:32:52.84 ATV.dial PCR @20:32:53.49 ASV.dial :waste1 @20:32:53.51 ARV.dial :air1 @20:32:53.97 Positioning Slug by 0.324ml @20:32:54.10 AS.seek 0.326ml @20:38:20.12 delay 5 seconds @20:38:25.15 ATV.dial ARV @20:38:25.96 ASV.dial :PCRlock @20:38:26.38 delay 5 seconds @20:38:31.41 Running CG Profile60C300 thermocycling @20:38:31.43 PCR.program Profile60C300 @20:38:31.58 Profile60C300 should complete in < 1:59:25 @20:38:31.60 PCR.run 2:34:16.25 @22:35:28.55 PCR.off @22:35:29.87 PCR for CG completed -- closed /var/log/esp/CG20jul1420h.pcr @22:35:29.98 Cleaning PCR @22:35:30.00 AFV.select :carrierSolution @22:35:30.16 delay 2 seconds @22:35:32.24 AS.reconfigure ASconfig @22:35:32.33 AS.seek 0.5ml @22:35:42.67 delay 1 second @22:35:43.69 AFV.select :holdingCoil @22:35:43.81 ASV.dial :waste1 @22:35:44.22 ATV.dial :air3 @22:35:45.57 AS.reconfigure ASslow1 @22:35:45.66 AS.seek 0.51ml @22:35:48.00 delay 2 seconds @22:35:50.02 ATV.dial :bleach @22:35:50.67 AS.seek 0.81ml @22:36:51.30 delay 3 seconds @22:36:54.33 ATV.dial :air3 @22:36:54.91 delay 3 seconds @22:36:58.00 AS.seek 0.835ml @22:37:03.26 delay 3 seconds @22:37:06.29 ATV.dial :bleach @22:37:06.95 AS.seek 0.82ml @22:37:10.28 delay 3 seconds @22:37:13.31 ARV.dial :waste @22:37:13.96 delay 3 seconds @22:37:16.99 ATV.dial :ARV @22:37:18.02 AS.reconfigure ASconfig @22:37:18.11 AS.seek 0.47ml @22:37:38.52 AFV.select :carrierSolution @22:37:38.66 delay 2 seconds @22:37:40.69 AS.seek 1ml @22:38:11.47 delay 1 second @22:38:12.49 AFV.select :holdingCoil @22:38:12.63 delay 1 second @22:38:13.66 AS.reconfigure ASfast @22:38:13.74 AS.seek 0ml @22:38:41.12 AFV.select :carrierSolution @22:38:41.24 delay 2 seconds @22:38:43.26 AS.reconfigure ASconfig @22:38:43.33 AS.seek 1ml @22:39:41.08 delay 1 second @22:39:42.11 AFV.select :holdingCoil @22:39:42.23 delay 1 second @22:39:43.26 AS.reconfigure ASfast @22:39:43.34 AS.seek 0ml @22:40:10.73 AFV.select :carrierSolution @22:40:10.87 delay 2 seconds @22:40:12.90 AS.reconfigure ASconfig @22:40:12.99 AS.seek 1ml @22:41:10.81 delay 1 second @22:41:11.83 AFV.select :holdingCoil @22:41:11.96 delay 1 second @22:41:12.99 AS.reconfigure ASfast @22:41:13.07 AS.seek 0ml @22:41:40.43 ARV.dial between carrier and air1 @22:41:40.99 ATV.dial :air3 @22:41:42.33 AS.reconfigure ASslow1 @22:41:42.42 AS.seek 0.01ml @22:41:44.75 delay 2 seconds @22:41:46.77 ATV.dial :bleach @22:41:47.43 AS.seek 0.81ml @22:44:28.61 delay 3 seconds @22:44:31.65 ATV.dial :air3 @22:44:32.24 delay 3 seconds @22:44:35.33 AS.seek 0.835ml @22:44:40.58 delay 3 seconds @22:44:43.60 ATV.dial :bleach @22:44:44.24 AS.seek 0.82ml @22:44:47.58 delay 3 seconds @22:44:50.60 ATV.dial PCR @22:44:51.02 AS.reconfigure ASconfig @22:44:51.11 AS.seek :empty @22:45:38.58 PCR.program :bleachProfile @22:45:38.66 PCR program should complete in < 2:45 @22:45:38.68 PCR.run 10:00 @22:48:28.42 PCR.off @22:48:28.47 AFV.select :carrierSolution @22:48:28.62 delay 2 seconds @22:48:30.65 AS.seek 1ml @22:49:28.50 delay 1 second @22:49:29.52 AFV.select :holdingCoil @22:49:29.65 delay 1 second @22:49:30.68 AS.reconfigure ASfast @22:49:30.77 AS.seek 0ml @22:49:58.16 delay 3 seconds @22:50:01.19 AFV.select :carrierSolution @22:50:01.32 delay 2 seconds @22:50:03.35 AS.reconfigure ASconfig @22:50:04.02 AS.seek 1ml @22:51:01.84 delay 1 second @22:51:02.86 AFV.select :holdingCoil @22:51:02.99 delay 1 second @22:51:04.02 AS.reconfigure ASfast @22:51:04.11 AS.seek 0ml @22:51:31.49 delay 3 seconds @22:51:34.52 AFV.select :carrierSolution @22:51:34.66 delay 2 seconds @22:51:36.69 AS.reconfigure ASconfig @22:51:36.79 AS.seek 1ml @22:52:34.63 delay 1 second @22:52:35.65 AFV.select :holdingCoil @22:52:35.77 delay 1 second @22:52:36.80 AS.reconfigure ASfast @22:52:36.88 AS.seek 0ml @22:53:04.28 delay 3 seconds @22:53:07.31 AFV.select :carrierSolution @22:53:07.43 delay 2 seconds @22:53:09.46 AS.reconfigure ASconfig @22:53:09.55 AS.seek 1ml @22:54:07.38 delay 1 second @22:54:08.40 AFV.select :holdingCoil @22:54:08.52 delay 1 second @22:54:09.55 AS.reconfigure ASfast @22:54:09.63 AS.seek 0ml @22:54:37.02 delay 3 seconds @22:54:40.05 AFV.select :carrierSolution @22:54:40.19 delay 2 seconds @22:54:42.22 AS.reconfigure ASconfig @22:54:42.32 AS.seek 1ml @22:55:40.14 delay 1 second @22:55:41.16 AFV.select :holdingCoil @22:55:41.29 delay 1 second @22:55:42.32 AS.reconfigure ASnorm @22:55:42.41 AS.seek 0ml @22:56:33.18 Setting up PCR for NM -- writing /var/log/esp/NM20jul1422h.pcr @22:56:33.21 Building PCR Slug from ASV with MM1 mix, PP1 primer @22:56:33.23 Priming with carrier @22:56:33.29 ATV.dial ARV @22:56:33.95 ARV.dial :carrier @22:56:35.35 AS.seek 0.5ml @22:57:01.01 AFV.select :carrierSolution @22:57:01.17 delay 2 seconds @22:57:03.20 AS.reconfigure ASconfig @22:57:03.29 AS.seek 1ml @22:57:32.33 delay 1 second @22:57:33.35 AFV.select :holdingCoil @22:57:33.47 delay 1 second @22:57:34.50 ATV.dial :waste @22:57:36.20 AS.reconfigure ASnorm @22:57:36.29 AS.seek :empty @22:58:27.05 AFV.select :carrierSolution @22:58:27.21 delay 2 seconds @22:58:29.24 AS.reconfigure ASconfig @22:58:29.33 AS.seek 0.5ml @22:58:58.44 delay 1 second @22:58:59.46 AFV.select :holdingCoil @22:58:59.59 Building Slug @22:58:59.61 ATV.dial :ARV @22:59:01.31 delay 2 seconds @22:59:03.34 ARV.dial :air1 @22:59:03.77 delay 2 seconds @22:59:05.85 AS.reconfigure ASslow2 @22:59:05.95 AS.seek 0.506ml @22:59:12.31 delay 2 seconds @22:59:14.33 ARV.dial :MM1 @22:59:14.94 delay 2 seconds @22:59:16.97 Adding MM1 @22:59:17.08 AS.seek 0.524ml @22:59:35.69 delay 25 seconds @23:00:00.72 ARV.dial :PP1 @23:00:01.16 Adding PP1 @23:00:01.18 delay 2 seconds @23:00:03.29 AS.seek 0.53ml @23:00:09.50 delay 10 seconds @23:00:19.53 ARV.dial :air1 @23:00:19.94 adding ARV air @23:00:19.96 delay 2 seconds @23:00:22.07 AS.seek 0.536ml @23:00:28.45 delay 5 seconds @23:00:33.49 ARV.dial :carrier @23:00:33.92 delay 7 seconds @23:00:41.01 AS.seek 0.594ml @23:01:39.72 delay 5 seconds @23:01:44.75 ATV.dial :ASV @23:01:45.19 delay 2 seconds @23:01:47.28 AS.seek 0.6ml @23:01:53.58 delay 4 seconds @23:01:57.61 ATV.dial ARV @23:01:58.20 delay 2 seconds @23:02:00.23 ARV.dial :carrier @23:02:00.25 delay 2 seconds @23:02:02.35 AS.seek 0.65ml @23:02:52.89 delay 4 seconds @23:02:56.92 ATV.dial PCR @23:02:57.55 ASV.dial :waste1 @23:02:57.57 ARV.dial :air1 @23:02:58.03 Positioning Slug by 0.324ml @23:02:58.16 AS.seek 0.326ml @23:08:24.27 delay 5 seconds @23:08:29.30 ATV.dial ARV @23:08:30.70 ASV.dial :PCRlock @23:08:31.13 delay 5 seconds @23:08:36.16 Running NM Profile60C300 thermocycling @23:08:36.17 PCR.program Profile60C300 @23:08:36.32 Profile60C300 should complete in < 1:59:25 @23:08:36.34 PCR.run 2:34:16.25 @01:05:58.53CEST15-Jul-20 PCR.off @01:05:59.23 PCR for NM completed -- closed /var/log/esp/NM20jul1422h.pcr @01:05:59.34 Cleaning PCR @01:05:59.35 AFV.select :carrierSolution @01:05:59.51 delay 2 seconds @01:06:01.54 AS.reconfigure ASconfig @01:06:01.63 AS.seek 0.5ml @01:06:11.98 delay 1 second @01:06:13.00 AFV.select :holdingCoil @01:06:13.13 ASV.dial :waste1 @01:06:13.54 ATV.dial :air3 @01:06:14.88 AS.reconfigure ASslow1 @01:06:14.98 AS.seek 0.51ml @01:06:17.28 delay 2 seconds @01:06:19.30 ATV.dial :bleach @01:06:19.97 AS.seek 0.81ml @01:07:20.63 delay 3 seconds @01:07:23.66 ATV.dial :air3 @01:07:24.23 delay 3 seconds @01:07:27.31 AS.seek 0.835ml @01:07:32.57 delay 3 seconds @01:07:35.59 ATV.dial :bleach @01:07:36.24 AS.seek 0.82ml @01:07:39.59 delay 3 seconds @01:07:42.61 ARV.dial :waste @01:07:43.26 delay 3 seconds @01:07:46.29 ATV.dial :ARV @01:07:47.23 AS.reconfigure ASconfig @01:07:47.32 AS.seek 0.47ml @01:08:07.78 AFV.select :carrierSolution @01:08:07.92 delay 2 seconds @01:08:09.95 AS.seek 1ml @01:08:40.77 delay 1 second @01:08:41.79 AFV.select :holdingCoil @01:08:41.93 delay 1 second @01:08:42.96 AS.reconfigure ASfast @01:08:43.05 AS.seek 0ml @01:09:10.44 AFV.select :carrierSolution @01:09:10.58 delay 2 seconds @01:09:12.61 AS.reconfigure ASconfig @01:09:12.70 AS.seek 1ml @01:10:10.48 delay 1 second @01:10:11.50 AFV.select :holdingCoil @01:10:11.63 delay 1 second @01:10:12.66 AS.reconfigure ASfast @01:10:12.74 AS.seek 0ml @01:10:40.11 AFV.select :carrierSolution @01:10:40.25 delay 2 seconds @01:10:42.28 AS.reconfigure ASconfig @01:10:42.37 AS.seek 1ml @01:11:40.15 delay 1 second @01:11:41.17 AFV.select :holdingCoil @01:11:41.30 delay 1 second @01:11:42.33 AS.reconfigure ASfast @01:11:42.42 AS.seek 0ml @01:12:09.81 ARV.dial between carrier and air1 @01:12:10.35 ATV.dial :air3 @01:12:11.69 AS.reconfigure ASslow1 @01:12:11.78 AS.seek 0.01ml @01:12:14.13 delay 2 seconds @01:12:16.15 ATV.dial :bleach @01:12:16.81 AS.seek 0.81ml @01:14:57.98 delay 3 seconds @01:15:01.00 ATV.dial :air3 @01:15:01.59 delay 3 seconds @01:15:05.26 AS.seek 0.835ml @01:15:10.51 delay 3 seconds @01:15:13.54 ATV.dial :bleach @01:15:14.20 AS.seek 0.82ml @01:15:17.52 delay 3 seconds @01:15:20.55 ATV.dial PCR @01:15:20.99 AS.reconfigure ASconfig @01:15:21.08 AS.seek :empty @01:16:08.57 PCR.program :bleachProfile @01:16:08.65 PCR program should complete in < 2:45 @01:16:08.67 PCR.run 10:00 @01:18:58.46 PCR.off @01:18:58.52 AFV.select :carrierSolution @01:18:58.66 delay 2 seconds @01:19:00.70 AS.seek 1ml @01:19:58.55 delay 1 second @01:19:59.57 AFV.select :holdingCoil @01:19:59.70 delay 1 second @01:20:00.73 AS.reconfigure ASfast @01:20:00.81 AS.seek 0ml @01:20:28.29 delay 3 seconds @01:20:31.32 AFV.select :carrierSolution @01:20:31.45 delay 2 seconds @01:20:33.49 AS.reconfigure ASconfig @01:20:33.58 AS.seek 1ml @01:21:31.41 delay 1 second @01:21:32.43 AFV.select :holdingCoil @01:21:32.55 delay 1 second @01:21:33.58 AS.reconfigure ASfast @01:21:33.66 AS.seek 0ml @01:22:01.05 delay 3 seconds @01:22:04.08 AFV.select :carrierSolution @01:22:04.21 delay 2 seconds @01:22:06.24 AS.reconfigure ASconfig @01:22:06.34 AS.seek 1ml @01:23:04.16 delay 1 second @01:23:05.18 AFV.select :holdingCoil @01:23:05.30 delay 1 second @01:23:06.33 AS.reconfigure ASfast @01:23:06.42 AS.seek 0ml @01:23:33.79 delay 3 seconds @01:23:36.82 AFV.select :carrierSolution @01:23:36.94 delay 2 seconds @01:23:38.97 AS.reconfigure ASconfig @01:23:39.07 AS.seek 1ml @01:24:36.88 delay 1 second @01:24:37.90 AFV.select :holdingCoil @01:24:38.03 delay 1 second @01:24:39.06 AS.reconfigure ASfast @01:24:39.14 AS.seek 0ml @01:25:06.51 delay 3 seconds @01:25:09.54 AFV.select :carrierSolution @01:25:09.68 delay 2 seconds @01:25:11.71 AS.reconfigure ASconfig @01:25:11.81 AS.seek 1ml @01:26:09.60 delay 1 second @01:26:10.62 AFV.select :holdingCoil @01:26:10.75 delay 1 second @01:26:11.78 AS.reconfigure ASnorm @01:26:11.87 AS.seek 0ml @01:27:02.65 Setting up PCR for IPc -- writing /var/log/esp/IPc20jul1501h.pcr @01:27:02.68 Building PCR Slug from ASV with MM3 mix, PP6 primer @01:27:02.73 Priming with carrier @01:27:02.75 ATV.dial ARV @01:27:03.43 ARV.dial :carrier @01:27:03.83 AS.seek 0.5ml @01:27:29.39 AFV.select :carrierSolution @01:27:29.54 delay 2 seconds @01:27:31.57 AS.reconfigure ASconfig @01:27:31.66 AS.seek 1ml @01:28:00.70 delay 1 second @01:28:01.72 AFV.select :holdingCoil @01:28:01.86 delay 1 second @01:28:02.89 ATV.dial :waste @01:28:04.60 AS.reconfigure ASnorm @01:28:04.69 AS.seek :empty @01:28:55.44 AFV.select :carrierSolution @01:28:55.58 delay 2 seconds @01:28:57.61 AS.reconfigure ASconfig @01:28:57.70 AS.seek 0.5ml @01:29:26.84 delay 1 second @01:29:27.86 AFV.select :holdingCoil @01:29:27.99 Building Slug @01:29:28.01 ATV.dial :ARV @01:29:29.71 delay 2 seconds @01:29:31.74 ARV.dial :air1 @01:29:32.17 delay 2 seconds @01:29:34.25 AS.reconfigure ASslow2 @01:29:34.34 AS.seek 0.506ml @01:29:40.65 delay 2 seconds @01:29:42.68 ARV.dial :MM3 @01:29:43.79 delay 2 seconds @01:29:45.82 Adding MM3 @01:29:45.94 AS.seek 0.524ml @01:30:04.41 delay 25 seconds @01:30:29.44 ARV.dial :PP6 @01:30:29.87 Adding PP6 @01:30:29.88 delay 2 seconds @01:30:31.99 AS.seek 0.53ml @01:30:38.80 delay 10 seconds @01:30:48.83 ARV.dial :air1 @01:30:49.70 adding ARV air @01:30:49.72 delay 2 seconds @01:30:51.83 AS.seek 0.536ml @01:30:58.02 delay 5 seconds @01:31:03.05 ARV.dial :carrier @01:31:03.51 delay 7 seconds @01:31:10.60 AS.seek 0.594ml @01:32:09.21 delay 5 seconds @01:32:14.24 ATV.dial :ASV @01:32:14.68 delay 2 seconds @01:32:16.77 AS.seek 0.6ml @01:32:23.07 delay 4 seconds @01:32:27.10 ATV.dial ARV @01:32:27.70 delay 2 seconds @01:32:29.73 ARV.dial :carrier @01:32:29.75 delay 2 seconds @01:32:31.84 AS.seek 0.65ml @01:33:22.55 delay 4 seconds @01:33:26.58 ATV.dial PCR @01:33:27.23 ASV.dial :waste1 @01:33:27.25 ARV.dial :air1 @01:33:27.70 Positioning Slug by 0.324ml @01:33:27.83 AS.seek 0.326ml @01:38:53.91 delay 5 seconds @01:38:58.94 ATV.dial ARV @01:38:59.76 ASV.dial :PCRlock @01:39:00.18 delay 5 seconds @01:39:05.21 Running IPc ProfileIPc thermocycling @01:39:05.22 PCR.program ProfileIPc @01:39:05.37 ProfileIPc should complete in < 1:36:15 @01:39:05.39 PCR.run 2:05:18.75 @03:18:16.49 PCR.off @03:18:17.04 PCR for IPc completed -- closed /var/log/esp/IPc20jul1501h.pcr @03:18:17.12 Cleaning PCR @03:18:17.14 AFV.select :carrierSolution @03:18:17.28 delay 2 seconds @03:18:19.30 AS.reconfigure ASconfig @03:18:19.38 AS.seek 0.5ml @03:18:29.70 delay 1 second @03:18:30.72 AFV.select :holdingCoil @03:18:30.85 ASV.dial :waste1 @03:18:31.27 ATV.dial :air3 @03:18:32.61 AS.reconfigure ASslow1 @03:18:32.70 AS.seek 0.51ml @03:18:35.03 delay 2 seconds @03:18:37.05 ATV.dial :bleach @03:18:37.71 AS.seek 0.81ml @03:19:38.94 delay 3 seconds @03:19:41.97 ATV.dial :air3 @03:19:42.55 delay 3 seconds @03:19:45.64 AS.seek 0.835ml @03:19:50.98 delay 3 seconds @03:19:54.01 ATV.dial :bleach @03:19:54.67 AS.seek 0.82ml @03:19:58.02 delay 3 seconds @03:20:01.05 ARV.dial :waste @03:20:01.69 delay 3 seconds @03:20:04.72 ATV.dial :ARV @03:20:05.66 AS.reconfigure ASconfig @03:20:05.75 AS.seek 0.47ml @03:20:26.18 AFV.select :carrierSolution @03:20:26.32 delay 2 seconds @03:20:28.36 AS.seek 1ml @03:20:59.16 delay 1 second @03:21:00.18 AFV.select :holdingCoil @03:21:00.30 delay 1 second @03:21:01.33 AS.reconfigure ASfast @03:21:01.41 AS.seek 0ml @03:21:28.79 AFV.select :carrierSolution @03:21:28.93 delay 2 seconds @03:21:30.96 AS.reconfigure ASconfig @03:21:31.05 AS.seek 1ml @03:22:28.84 delay 1 second @03:22:29.86 AFV.select :holdingCoil @03:22:29.99 delay 1 second @03:22:31.02 AS.reconfigure ASfast @03:22:31.10 AS.seek 0ml @03:22:58.48 AFV.select :carrierSolution @03:22:58.62 delay 2 seconds @03:23:00.65 AS.reconfigure ASconfig @03:23:00.73 AS.seek 1ml @03:23:58.55 delay 1 second @03:23:59.57 AFV.select :holdingCoil @03:23:59.71 delay 1 second @03:24:00.74 AS.reconfigure ASfast @03:24:00.82 AS.seek 0ml @03:24:28.18 ARV.dial between carrier and air1 @03:24:28.74 ATV.dial :air3 @03:24:30.08 AS.reconfigure ASslow1 @03:24:30.18 AS.seek 0.01ml @03:24:32.58 delay 2 seconds @03:24:34.60 ATV.dial :bleach @03:24:35.26 AS.seek 0.81ml @03:27:16.44 delay 3 seconds @03:27:19.47 ATV.dial :air3 @03:27:20.06 delay 3 seconds @03:27:23.14 AS.seek 0.835ml @03:27:28.40 delay 3 seconds @03:27:31.43 ATV.dial :bleach @03:27:32.10 AS.seek 0.82ml @03:27:35.36 delay 3 seconds @03:27:38.39 ATV.dial PCR @03:27:38.83 AS.reconfigure ASconfig @03:27:38.91 AS.seek :empty @03:28:26.41 PCR.program :bleachProfile @03:28:26.50 PCR program should complete in < 2:45 @03:28:26.52 PCR.run 10:00 @03:31:16.26 PCR.off @03:31:16.32 AFV.select :carrierSolution @03:31:16.46 delay 2 seconds @03:31:18.49 AS.seek 1ml @03:32:16.31 delay 1 second @03:32:17.33 AFV.select :holdingCoil @03:32:17.46 delay 1 second @03:32:18.49 AS.reconfigure ASfast @03:32:18.58 AS.seek 0ml @03:32:45.97 delay 3 seconds @03:32:49.00 AFV.select :carrierSolution @03:32:49.12 delay 2 seconds @03:32:51.15 AS.reconfigure ASconfig @03:32:51.25 AS.seek 1ml @03:33:49.05 delay 1 second @03:33:50.07 AFV.select :holdingCoil @03:33:50.19 delay 1 second @03:33:51.22 AS.reconfigure ASfast @03:33:51.30 AS.seek 0ml @03:34:18.67 delay 3 seconds @03:34:21.70 AFV.select :carrierSolution @03:34:21.84 delay 2 seconds @03:34:23.87 AS.reconfigure ASconfig @03:34:23.97 AS.seek 1ml @03:35:21.77 delay 1 second @03:35:22.79 AFV.select :holdingCoil @03:35:22.92 delay 1 second @03:35:23.95 AS.reconfigure ASfast @03:35:24.04 AS.seek 0ml @03:35:51.41 delay 3 seconds @03:35:54.44 AFV.select :carrierSolution @03:35:54.58 delay 2 seconds @03:35:56.61 AS.reconfigure ASconfig @03:35:56.70 AS.seek 1ml @03:36:54.55 delay 1 second @03:36:55.57 AFV.select :holdingCoil @03:36:55.70 delay 1 second @03:36:57.22 AS.reconfigure ASfast @03:36:57.39 AS.seek 0ml @03:37:24.78 delay 3 seconds @03:37:27.81 AFV.select :carrierSolution @03:37:27.95 delay 2 seconds @03:37:29.98 AS.reconfigure ASconfig @03:37:30.08 AS.seek 1ml @03:38:27.87 delay 1 second @03:38:28.90 AFV.select :holdingCoil @03:38:29.03 delay 1 second @03:38:30.06 AS.reconfigure ASnorm @03:38:30.15 AS.seek 0ml @03:39:20.91 Setting up PCR for IPc2 -- writing /var/log/esp/IPc220jul1503h.pcr @03:39:20.94 Building PCR Slug from ASV with MM2 mix, PP3 primer @03:39:20.98 Priming with carrier @03:39:21.02 ATV.dial ARV @03:39:21.69 ARV.dial :carrier @03:39:22.09 AS.seek 0.5ml @03:39:47.63 AFV.select :carrierSolution @03:39:47.79 delay 2 seconds @03:39:49.82 AS.reconfigure ASconfig @03:39:49.91 AS.seek 1ml @03:40:18.97 delay 1 second @03:40:19.99 AFV.select :holdingCoil @03:40:20.13 delay 1 second @03:40:21.16 ATV.dial :waste @03:40:22.89 AS.reconfigure ASnorm @03:40:22.98 AS.seek :empty @03:41:13.77 AFV.select :carrierSolution @03:41:13.93 delay 2 seconds @03:41:15.96 AS.reconfigure ASconfig @03:41:16.05 AS.seek 0.5ml @03:41:45.19 delay 1 second @03:41:46.21 AFV.select :holdingCoil @03:41:46.34 Building Slug @03:41:46.36 ATV.dial :ARV @03:41:48.08 delay 2 seconds @03:41:50.11 ARV.dial :air1 @03:41:50.51 delay 2 seconds @03:41:52.59 AS.reconfigure ASslow2 @03:41:52.68 AS.seek 0.506ml @03:41:59.00 delay 2 seconds @03:42:01.02 ARV.dial :MM2 @03:42:02.56 delay 2 seconds @03:42:04.60 Adding MM2 @03:42:04.71 AS.seek 0.524ml @03:42:23.06 delay 25 seconds @03:42:48.09 ARV.dial :PP3 @03:42:48.52 Adding PP3 @03:42:48.54 delay 2 seconds @03:42:50.65 AS.seek 0.53ml @03:42:57.05 delay 10 seconds @03:43:07.08 ARV.dial :air2 @03:43:07.48 adding ARV air @03:43:07.50 delay 2 seconds @03:43:09.61 AS.seek 0.536ml @03:43:15.89 delay 5 seconds @03:43:20.92 ARV.dial :carrier @03:43:22.25 delay 7 seconds @03:43:29.34 AS.seek 0.594ml @03:44:27.86 delay 5 seconds @03:44:32.89 ATV.dial :ASV @03:44:33.33 delay 2 seconds @03:44:35.42 AS.seek 0.6ml @03:44:41.66 delay 4 seconds @03:44:45.69 ATV.dial ARV @03:44:46.28 delay 2 seconds @03:44:48.31 ARV.dial :carrier @03:44:48.33 delay 2 seconds @03:44:50.43 AS.seek 0.65ml @03:45:41.12 delay 4 seconds @03:45:45.15 ATV.dial PCR @03:45:45.80 ASV.dial :waste1 @03:45:45.82 ARV.dial :air1 @03:45:46.28 Positioning Slug by 0.324ml @03:45:46.40 AS.seek 0.326ml @03:51:12.33 delay 5 seconds @03:51:17.36 ATV.dial ARV @03:51:18.17 ASV.dial :PCRlock @03:51:18.59 delay 5 seconds @03:51:23.62 Running IPc2 ProfileIPc thermocycling @03:51:23.64 PCR.program ProfileIPc @03:51:23.79 ProfileIPc should complete in < 1:36:15 @03:51:23.81 PCR.run 2:05:18.75 @05:29:35.09 PCR.off @05:29:35.66 PCR for IPc2 completed -- closed /var/log/esp/IPc220jul1503h.pcr @05:29:35.68 Full MFB Decontamination @05:29:35.73 Decon extraction column and decon AIV @05:29:35.79 SPE.hold 2:46:40,55C @05:29:35.85 ASV.dial :SPEdelivery @05:29:36.56 delay 20 seconds @05:29:56.59 AFV.select :carrierSolution @05:29:56.72 delay 2 seconds @05:29:58.75 AS.reconfigure ASconfig @05:29:58.83 AS.seek 0.5ml @05:30:09.17 delay 1 second @05:30:10.19 AFV.select :holdingCoil @05:30:10.31 ATV.dial :air1 @05:30:12.58 AS.reconfigure ASslow1 @05:30:12.67 AS.seek 0.52ml @05:30:16.97 ATV.dial :ASV @05:30:18.74 ASV.dial :SPErecovery @05:30:19.49 AS.seek 0.62ml @05:30:39.91 ATV.dial :waste @05:30:42.12 AS.reconfigure ASnorm @05:30:42.21 AS.seek :empty @05:31:13.89 AFV.select :holdingCoil @05:31:13.91 ATV.dial :waste @05:31:13.96 AS.reconfigure ASconfig @05:31:14.06 AS.seek 0ml @05:31:14.37 AFV.select :carrierSolution @05:31:14.51 delay 2 seconds @05:31:16.55 AS.seek 0.5ml @05:31:45.60 delay 1 second @05:31:46.62 AFV.select :holdingCoil @05:31:46.75 ATV.dial :air3 @05:31:47.55 AS.reconfigure ASslow1 @05:31:47.63 AS.seek 0.52ml @05:31:51.98 ATV.dial :air3 @05:31:52.10 AS.seek 0.53ml @05:31:54.35 delay 2 seconds @05:31:56.37 ATV.dial :bleach @05:31:56.91 AS.seek 0.71ml @05:32:33.45 delay 3 seconds @05:32:36.48 ATV.dial :air3 @05:32:37.09 delay 3 seconds @05:32:40.18 AS.seek 0.735ml @05:32:45.43 delay 3 seconds @05:32:48.46 ATV.dial :bleach @05:32:49.14 AS.seek 0.72ml @05:32:52.48 delay 3 seconds @05:32:55.51 ATV.dial ASV @05:32:56.70 ASV.dial :SPErecovery @05:32:56.82 AS.seek 0.365ml @05:34:08.52 ATV.dial :waste @05:34:10.46 AS.reconfigure ASnorm @05:34:10.55 AS.seek :empty @05:34:29.37 ATV.dial :air3 @05:34:30.13 AS.reconfigure ASslow1 @05:34:30.21 AS.seek 0.009ml @05:34:32.59 delay 2 seconds @05:34:34.61 ATV.dial :bleach @05:34:35.13 AS.seek 0.579ml @05:36:30.06 delay 3 seconds @05:36:33.09 ATV.dial :air3 @05:36:33.71 delay 3 seconds @05:36:36.80 AS.seek 0.604ml @05:36:42.16 delay 3 seconds @05:36:45.19 ATV.dial :bleach @05:36:45.86 AS.seek 0.589ml @05:36:49.23 delay 3 seconds @05:36:52.26 ATV.dial :ASV @05:36:53.37 ASV.dial :wasteR @05:36:53.89 AS.seek 0ml @05:38:52.70 delay 1:00 @05:39:52.73 ATV.dial ASV @05:39:52.74 AFV.select :carrierSolution @05:39:52.91 delay 2 seconds @05:39:54.94 AS.reconfigure ASconfig @05:39:55.02 AS.seek 1ml @05:40:52.81 delay 1 second @05:40:53.83 AFV.select :holdingCoil @05:40:53.96 delay 1 second @05:40:54.99 ASV.dial :wasteR @05:40:55.01 AS.reconfigure ASslow1 @05:40:55.11 AS.seek 0ml @05:44:16.52 delay 10 seconds @05:44:26.55 ATV.dial :air3 @05:44:28.15 AS.seek 0.01ml @05:44:30.48 delay 2 seconds @05:44:32.50 ATV.dial :bleach @05:44:33.17 AS.seek 0.58ml @05:46:28.09 delay 3 seconds @05:46:31.12 ATV.dial :air3 @05:46:31.73 delay 3 seconds @05:46:34.82 AS.seek 0.605ml @05:46:40.14 delay 3 seconds @05:46:43.17 ATV.dial :bleach @05:46:43.84 AS.seek 0.59ml @05:46:47.18 delay 3 seconds @05:46:50.21 ATV.dial ASV @05:46:51.29 ASV.dial :SPEdelivery @05:46:51.71 AS.seek 0ml @05:48:50.65 delay 1:00 @05:49:50.68 AFV.select :carrierSolution @05:49:50.81 delay 2 seconds @05:49:52.84 AS.reconfigure ASconfig @05:49:52.93 AS.seek 1ml @05:50:50.73 delay 1 second @05:50:51.75 AFV.select :holdingCoil @05:50:51.88 delay 1 second @05:50:52.91 ATV.dial ASV @05:50:52.92 ASV.dial :SPEdelivery @05:50:52.98 AS.reconfigure ASslow1 @05:50:53.07 AS.seek 0ml @05:54:14.48 delay 10 seconds @05:54:24.51 ATV.dial :air2 @05:54:25.38 AS.reconfigure ASslow0 @05:54:25.47 AS.seek 0.8ml @05:55:46.30 delay 3 seconds @05:55:49.33 ATV.dial :ASV @05:55:50.37 ASV.dial :SPEdelivery @05:55:50.40 AS.reconfigure ASslow1 @05:55:50.50 AS.seek 0.03ml @05:58:25.66 delay 20 seconds @05:58:45.69 ATV.dial :waste,via: :air3 @05:58:47.51 AS.reconfigure ASconfig @05:58:47.60 AS.seek :empty @05:58:49.70 SPE.coast @05:58:49.82 Removing Bleach from Mixing Coil @05:58:49.83 AFV.select :carrierSolution @05:58:49.98 delay 2 seconds @05:58:52.02 AS.seek 0.5ml @05:59:21.15 delay 1 second @05:59:22.17 AFV.select :holdingCoil @05:59:22.30 ATV.dial :ASV @05:59:24.07 ASV.dial :SPErecovery @05:59:24.74 AS.reconfigure ASslow1 @05:59:24.83 AS.seek 0.73ml @06:00:11.36 ASV.dial :wasteD @06:00:11.99 AS.reconfigure ASnorm @06:00:12.08 AS.seek 0ml @06:00:49.21 AFV.select :carrierSolution @06:00:49.34 delay 2 seconds @06:00:51.37 AS.reconfigure ASconfig @06:00:51.45 AS.seek 0.5ml @06:01:20.57 delay 1 second @06:01:21.59 AFV.select :holdingCoil @06:01:21.72 ATV.dial ASV @06:01:21.74 ASV.dial :SPErecovery @06:01:22.43 AS.reconfigure ASslow0 @06:01:22.53 AS.seek 0.3ml @06:01:43.00 ATV.dial :air2 @06:01:44.08 AS.reconfigure ASslow1 @06:01:44.17 AS.seek 0.32ml @06:01:48.53 ATV.dial ASV @06:01:50.23 AS.reconfigure ASslow0 @06:01:50.32 AS.seek 0.57ml @06:02:15.84 ASV.dial :wasteD @06:02:16.46 AS.reconfigure ASnorm @06:02:16.55 AS.seek 0ml @06:02:45.65 AFV.select :carrierSolution @06:02:45.79 delay 2 seconds @06:02:47.82 AS.reconfigure ASconfig @06:02:47.91 AS.seek 0.5ml @06:03:16.99 delay 1 second @06:03:18.01 AFV.select :holdingCoil @06:03:18.14 ATV.dial ASV @06:03:18.16 ASV.dial :SPErecovery @06:03:18.86 AS.reconfigure ASslow0 @06:03:18.94 AS.seek 0.3ml @06:03:39.40 ATV.dial :air2 @06:03:40.50 AS.reconfigure ASslow1 @06:03:40.58 AS.seek 0.32ml @06:03:44.95 ATV.dial ASV @06:03:46.05 AS.reconfigure ASslow0 @06:03:46.13 AS.seek 0.57ml @06:04:11.53 ASV.dial :wasteD @06:04:12.16 AS.reconfigure ASnorm @06:04:12.25 AS.seek 0ml @06:04:41.33 ATV.dial :air2 @06:04:42.37 AS.reconfigure ASslow0 @06:04:42.46 AS.seek 0.3ml @06:05:13.06 ATV.dial :ASV @06:05:14.10 ASV.dial :wasteD @06:05:14.13 AS.reconfigure ASconfig @06:05:14.23 AS.seek 0.05ml @06:05:28.94 ATV.dial :waste @06:05:30.87 AS.seek 0ml @06:05:34.11 ASV.dial :SPEdelivery @06:05:35.13 deconLysateINT @06:05:35.15 ATV.dial :air3 @06:05:35.91 AS.reconfigure ASslow1 @06:05:35.99 AS.seek 0.01ml @06:05:38.35 delay 2 seconds @06:05:40.37 ATV.dial :bleach @06:05:40.86 AS.seek 0.96ml @06:08:52.19 delay 3 seconds @06:08:55.22 ATV.dial :air3 @06:08:55.82 delay 3 seconds @06:08:58.91 AS.seek 0.985ml @06:09:04.27 delay 3 seconds @06:09:07.30 ATV.dial :bleach @06:09:07.95 AS.seek 0.97ml @06:09:11.32 delay 3 seconds @06:09:14.35 ATV.dial AIV @06:09:15.66 AIV.dial :coilToMFB @06:09:15.68 AWV.select :waste @06:09:15.72 AFV.select :carrierSolution @06:09:15.88 delay 2 seconds @06:09:17.91 AS.reconfigure ASconfig @06:09:18.00 AS.seek 1ml @06:09:20.05 delay 1 second @06:09:21.07 AFV.select :holdingCoil @06:09:21.20 delay 1 second @06:09:22.23 AS.reconfigure ASslow0 @06:09:22.32 AS.seek :empty @06:11:03.31 AFV.select :carrierSolution @06:11:03.45 delay 2 seconds @06:11:05.48 AS.reconfigure ASconfig @06:11:05.57 AS.seek 1ml @06:12:03.41 delay 1 second @06:12:04.43 AFV.select :holdingCoil @06:12:04.56 delay 1 second @06:12:05.59 AS.reconfigure ASslow0 @06:12:05.68 AS.seek :empty @06:13:46.68 AFV.select :carrierSolution @06:13:46.82 delay 2 seconds @06:13:48.85 AS.reconfigure ASconfig @06:13:48.94 AS.seek 1ml @06:14:46.76 delay 1 second @06:14:47.78 AFV.select :holdingCoil @06:14:47.91 delay 1 second @06:14:48.94 AS.reconfigure ASslow0 @06:14:49.03 AS.seek :empty @06:16:30.01 ATV.dial :air2 @06:16:30.75 AS.reconfigure ASslow1 @06:16:30.83 AS.seek 0.009ml @06:16:33.18 AS.reconfigure ASslow0 @06:16:33.27 AS.seek 1ml @06:18:13.25 ATV.dial AIV @06:18:14.08 AS.seek 0.02ml @06:19:52.93 ATV.dial :waste @06:19:54.64 AS.seek 0ml @06:19:57.01 AWV.select :air @06:19:57.79 Cleaning PCR @06:19:57.80 AFV.select :carrierSolution @06:19:57.97 delay 2 seconds @06:20:00.01 AS.reconfigure ASconfig @06:20:00.10 AS.seek 0.5ml @06:20:29.18 delay 1 second @06:20:30.20 AFV.select :holdingCoil @06:20:30.32 ASV.dial :waste1 @06:20:30.73 ATV.dial :air3 @06:20:31.47 AS.reconfigure ASslow1 @06:20:31.56 AS.seek 0.51ml @06:20:33.85 delay 2 seconds @06:20:35.87 ATV.dial :bleach @06:20:36.37 AS.seek 0.81ml @06:21:36.98 delay 3 seconds @06:21:40.01 ATV.dial :air3 @06:21:40.60 delay 3 seconds @06:21:43.69 AS.seek 0.835ml @06:21:49.06 delay 3 seconds @06:21:52.09 ATV.dial :bleach @06:21:52.75 AS.seek 0.82ml @06:21:56.07 delay 3 seconds @06:21:59.10 ARV.dial :waste @06:21:59.74 delay 3 seconds @06:22:02.77 ATV.dial :ARV @06:22:03.69 AS.reconfigure ASconfig @06:22:03.78 AS.seek 0.47ml @06:22:24.22 AFV.select :carrierSolution @06:22:24.37 delay 2 seconds @06:22:26.40 AS.seek 1ml @06:22:57.21 delay 1 second @06:22:58.23 AFV.select :holdingCoil @06:22:58.35 delay 1 second @06:22:59.38 AS.reconfigure ASfast @06:22:59.46 AS.seek 0ml @06:23:26.82 AFV.select :carrierSolution @06:23:26.96 delay 2 seconds @06:23:28.99 AS.reconfigure ASconfig @06:23:29.07 AS.seek 1ml @06:24:26.85 delay 1 second @06:24:27.87 AFV.select :holdingCoil @06:24:27.99 delay 1 second @06:24:29.02 AS.reconfigure ASfast @06:24:29.10 AS.seek 0ml @06:24:56.48 AFV.select :carrierSolution @06:24:56.61 delay 2 seconds @06:24:58.64 AS.reconfigure ASconfig @06:24:58.72 AS.seek 1ml @06:25:56.49 delay 1 second @06:25:57.51 AFV.select :holdingCoil @06:25:57.63 delay 1 second @06:25:58.66 AS.reconfigure ASfast @06:25:58.74 AS.seek 0ml @06:26:26.13 ARV.dial between carrier and air1 @06:26:26.66 ATV.dial :air3 @06:26:28.01 AS.reconfigure ASslow1 @06:26:28.09 AS.seek 0.01ml @06:26:30.48 delay 2 seconds @06:26:32.50 ATV.dial :bleach @06:26:33.15 AS.seek 0.81ml @06:29:14.32 delay 3 seconds @06:29:17.35 ATV.dial :air3 @06:29:17.93 delay 3 seconds @06:29:21.02 AS.seek 0.835ml @06:29:26.41 delay 3 seconds @06:29:29.44 ATV.dial :bleach @06:29:30.09 AS.seek 0.82ml @06:29:33.38 delay 3 seconds @06:29:36.40 ATV.dial PCR @06:29:36.83 AS.reconfigure ASconfig @06:29:36.92 AS.seek :empty @06:30:24.40 PCR.program :bleachProfile @06:30:24.48 PCR program should complete in < 2:45 @06:30:24.50 PCR.run 10:00 @06:33:14.24 PCR.off @06:33:14.30 AFV.select :carrierSolution @06:33:14.43 delay 2 seconds @06:33:16.46 AS.seek 1ml @06:34:14.29 delay 1 second @06:34:15.31 AFV.select :holdingCoil @06:34:15.44 delay 1 second @06:34:16.47 AS.reconfigure ASfast @06:34:16.55 AS.seek 0ml @06:34:43.94 delay 3 seconds @06:34:46.97 AFV.select :carrierSolution @06:34:47.11 delay 2 seconds @06:34:49.14 AS.reconfigure ASconfig @06:34:49.88 AS.seek 1ml @06:35:47.68 delay 1 second @06:35:48.70 AFV.select :holdingCoil @06:35:48.82 delay 1 second @06:35:49.85 AS.reconfigure ASfast @06:35:49.94 AS.seek 0ml @06:36:17.32 delay 3 seconds @06:36:20.35 AFV.select :carrierSolution @06:36:20.48 delay 2 seconds @06:36:22.51 AS.reconfigure ASconfig @06:36:22.59 AS.seek 1ml @06:37:20.40 delay 1 second @06:37:21.42 AFV.select :holdingCoil @06:37:21.55 delay 1 second @06:37:22.58 AS.reconfigure ASfast @06:37:22.67 AS.seek 0ml @06:37:50.06 delay 3 seconds @06:37:53.09 AFV.select :carrierSolution @06:37:53.20 delay 2 seconds @06:37:55.22 AS.reconfigure ASconfig @06:37:55.30 AS.seek 1ml @06:38:53.12 delay 1 second @06:38:54.15 AFV.select :holdingCoil @06:38:54.27 delay 1 second @06:38:55.30 AS.reconfigure ASfast @06:38:55.38 AS.seek 0ml @06:39:22.76 delay 3 seconds @06:39:25.79 AFV.select :carrierSolution @06:39:25.92 delay 2 seconds @06:39:27.95 AS.reconfigure ASconfig @06:39:28.03 AS.seek 1ml @06:40:25.82 delay 1 second @06:40:26.84 AFV.select :holdingCoil @06:40:26.97 delay 1 second @06:40:28.00 AS.reconfigure ASnorm @06:40:28.08 AS.seek 0ml @06:41:18.84 AFV.select :carrierSolution @06:41:18.98 delay 2 seconds @06:41:21.01 AS.reconfigure ASconfig @06:41:21.09 AS.seek 1ml @06:42:18.91 delay 1 second @06:42:19.93 AFV.select :holdingCoil @06:42:20.06 delay 1 second @06:42:21.09 ATV.dial :waste @06:42:22.33 AS.reconfigure ASnorm @06:42:22.42 AS.seek 0.5ml @06:42:48.01 ATV.dial :waste2 @06:42:49.38 AS.seek 0ml @06:43:14.98
Asleep after completing InvasivePostCoreNeg assays @06:43:15.00 Mission runtime was 17:04:33.917422 @06:43:15.05 GoodNews.email "{1 => 3, 2 => 0}\nCan@06:38:42, 23.4C, 49% humidity, 14.5psia, 12.031V, 0.563A, 0.569A avg, 6.77W",Subject: "Asleep after completing InvasivePostCoreNeg assays" @06:43:24.73 Session Ended @06:43:24.80 Session Ended @12:26:16.65CEST17-Jul-20
ESP2 v3.30 I2C Gateway v3.06 for core bus @12:26:18.57 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @12:26:18.87 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @12:26:23.78 Polling Can every 10:00 @12:26:25.60 SPE.coast @12:26:25.66 SPE.configure SPEconfig @12:26:25.76 AS.reconfigure ASconfig @12:26:25.80 AS.hold @12:26:25.86 AS.configure ASconfig @12:26:25.95 SS.reconfigure SSconfig @12:26:26.00 SS.hold @12:26:26.05 SS.configure SSconfig @12:26:26.15 CC.reconfigure CCconfig @12:26:26.19 CC.hold @12:26:26.26 CC.configure CCconfig @12:26:26.38 CS.reconfigure CSconfig @12:26:26.42 CS.hold @12:26:26.47 CS.configure CSconfig @12:26:26.94 PC.reconfigure PCconfig @12:26:27.01 PC.hold @12:26:27.08 PC.configure PCconfig @12:26:27.17 PS.configure PSconfig @12:26:27.29 Forearm.reconfigure LooseForearm @12:26:27.34 Forearm.hold @12:26:27.40 Forearm.configure LooseForearm @12:26:27.50 Elbow.reconfigure LooseElbow @12:26:27.54 Elbow.hold @12:26:27.60 Elbow.configure LooseElbow @12:26:27.71 SC.reconfigure SCloose @12:26:27.76 SC.hold @12:26:27.81 SC.configure SCloose @12:26:27.91 SE.reconfigure SEconfig @12:26:27.96 SE.hold @12:26:28.01 SE.configure SEconfig @12:26:28.06 CH.coast @12:26:28.11 CH.configure CHconfig @12:26:28.14 PH.coast @12:26:28.20 PH.configure PHconfig @12:26:28.24 PCR.off @12:26:28.27 PCR.pidtemp= 50,0.1,0,300,25,25 @12:26:28.29 PCR.caltemp= 2.8814,0.0372,0 @12:26:28.33 All dwarf controllers running firmware version 3.75 @12:26:28.49 Cleanly exited at 10:58:59CEST17-Jul-20 @12:26:28.56 Loading /home/esp/esp2/mission/stdMER2020MWJ.rb mission @12:26:29.29 AFV.select :carrierSolution @12:26:29.40 delay 2 seconds @12:26:31.43 AS.seek 0.5ml @12:26:34.13 Session Begun @12:27:00.51
delay 1 second @12:27:01.52 AFV.select :holdingCoil @12:27:01.64 ATV.dial :extSrc @12:27:03.17 AS.reconfigure ASslow1 @12:27:03.23 AS.seek 0.53ml @12:27:09.55 delay 5 seconds @12:27:14.64 AS.reconfigure ASslow2 @12:27:14.71 AS.seek 0.54ml @12:27:25.05 delay 20 seconds @12:27:45.13 AS.reconfigure ASslow1 @12:27:45.20 AS.seek 0.57ml @12:27:51.54 delay 5 seconds @12:27:56.64 AS.reconfigure ASslow2 @12:27:56.70 AS.seek 0.58ml @12:28:07.01 delay 20 seconds @12:28:27.09 AS.reconfigure ASslow1 @12:28:27.15 AS.seek 0.61ml @12:28:33.51 delay 5 seconds @12:28:38.60 AS.reconfigure ASslow2 @12:28:38.67 AS.seek 0.62ml @12:28:48.96 delay 20 seconds @12:29:09.04 AS.reconfigure ASslow1 @12:29:09.11 AS.seek 0.65ml @12:29:15.41 delay 5 seconds @12:29:20.51 AS.reconfigure ASslow2 @12:29:20.57 AS.seek 0.66ml @12:29:30.90 delay 20 seconds @12:29:50.93 AS.reconfigure ASslow1 @12:29:51.00 AS.seek 0.705ml @12:30:00.38 delay 10 seconds @12:30:10.41 ATV.dial :air1 @12:30:12.16 AS.seek 0.725ml @12:30:16.45 delay 3 seconds @12:30:19.48 ATV.dial :waste @12:30:20.04 AS.reconfigure ASslow0 @12:30:20.10 AS.seek :empty @12:31:33.57 Setting up PCR for OM -- writing /var/log/esp/OM20jul1712h.pcr @12:31:33.60 Building PCR Slug from extSrc with MM3 mix, PP5 primer @12:31:33.63 Priming with carrier @12:31:33.65 ATV.dial ARV @12:31:35.19 ARV.dial :carrier @12:31:35.53 AS.reconfigure ASnorm @12:31:35.61 AS.seek 0.499ml @12:32:01.22 AFV.select :carrierSolution @12:32:01.35 delay 2 seconds @12:32:03.38 AS.reconfigure ASconfig @12:32:03.45 AS.seek 1ml @12:32:32.52 delay 1 second @12:32:33.53 AFV.select :holdingCoil @12:32:33.66 delay 1 second @12:32:34.68 ATV.dial :waste @12:32:36.55 AS.reconfigure ASnorm @12:32:36.63 AS.seek :empty @12:33:27.50 AFV.select :carrierSolution @12:33:27.64 delay 2 seconds @12:33:29.67 AS.reconfigure ASconfig @12:33:29.74 AS.seek 0.5ml @12:33:58.85 delay 1 second @12:33:59.87 AFV.select :holdingCoil @12:34:00.00 Building Slug @12:34:00.01 ATV.dial :ARV @12:34:01.71 delay 2 seconds @12:34:03.73 ARV.dial :air1 @12:34:04.16 delay 2 seconds @12:34:06.24 AS.reconfigure ASslow2 @12:34:06.31 AS.seek 0.506ml @12:34:12.51 delay 2 seconds @12:34:14.53 ARV.dial :MM3 @12:34:15.63 delay 2 seconds @12:34:17.66 Adding MM3 @12:34:17.76 AS.seek 0.524ml @12:34:36.04 delay 25 seconds @12:35:01.07 ARV.dial :PP5 @12:35:01.49 Adding PP5 @12:35:01.51 delay 2 seconds @12:35:03.60 AS.seek 0.53ml @12:35:09.85 delay 10 seconds @12:35:19.88 ARV.dial :air3 @12:35:20.30 adding ARV air @12:35:20.32 delay 2 seconds @12:35:22.41 AS.seek 0.536ml @12:35:28.65 delay 5 seconds @12:35:33.68 ARV.dial :carrier @12:35:35.01 delay 7 seconds @12:35:42.10 AS.seek 0.594ml @12:36:40.61 delay 5 seconds @12:36:45.63 ATV.dial :extSrc @12:36:46.22 delay 2 seconds @12:36:48.31 AS.seek 0.6ml @12:36:54.70 delay 4 seconds @12:36:58.73 ATV.dial ARV @12:36:59.30 delay 2 seconds @12:37:01.32 ARV.dial :carrier @12:37:01.34 delay 2 seconds @12:37:03.44 AS.seek 0.65ml @12:37:54.30 delay 4 seconds @12:37:58.32 ATV.dial PCR @12:37:59.12 ASV.dial :waste1 @12:37:59.23 ARV.dial :air1 @12:38:00.13 Positioning Slug by 0.324ml @12:38:00.24 AS.seek 0.326ml @12:43:26.29 delay 5 seconds @12:43:31.31 ATV.dial ARV @12:43:32.11 ASV.dial :PCRlock @12:43:32.52 delay 5 seconds @12:43:37.55 Running OM Profile60C350 thermocycling @12:43:37.57 PCR.program Profile60C350 @12:43:37.70 Profile60C350 should complete in < 2:02:45 @12:43:37.71 PCR.run 2:38:26.25 @15:22:05.88 PCR.abort @15:22:05.91 PCR.off @15:22:05.95 AnalyticalModule::PCR::Aborted in PCR -- Program aborted due to timeout @15:22:06.00 BadNews.email "Program aborted due to timeout",Subject: "AnalyticalModule::PCR::Aborted in PCR" @15:22:06.03 Thread::ChildDied in MFBPCR -- :PCR @15:22:06.09 Waiting to Resume from Checkpoint ":PCR" @15:22:15.54 BadNews.email ":PCR",Subject: "Thread::ChildDied in MFBPCR" @21:02:10.16 Session Begun @21:02:21.68 -> showlog 10 @21:02:49.68 -> Abort! @21:02:49.71 NoMethodError in mwj#2 -- undefined method `Abort!' for # @21:02:58.48 -> abort! @21:02:58.51 CmdServer::Client::Error in mwj#2 -- Attempt to Abort Mission without specifying a reason! @21:03:19.60 -> abort! "PCR stopped" @21:03:19.63 CmdServer::Client::Error in mwj#2 -- Aborting Mission: PCR stopped @21:03:19.72 BadNews.email "PCR stopped",Subject: "Mission ABORTED!" @21:03:27.81 Session Ended @21:03:27.89 Session Ended @12:54:30.70CEST24-Jul-20
ESP2 v3.30 I2C Gateway v3.06 for core bus @12:54:32.94 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @12:54:33.36 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @12:54:39.68 Polling Can every 10:00 @12:54:41.97 SPE.coast @12:54:42.09 SPE.configure SPEconfig @12:54:42.21 AS.reconfigure ASconfig @12:54:42.25 AS.hold @12:54:42.31 AS.configure ASconfig @12:54:42.40 SS.reconfigure SSconfig @12:54:42.45 SS.hold @12:54:42.51 SS.configure SSconfig @12:54:42.61 CC.reconfigure CCconfig @12:54:42.65 CC.hold @12:54:42.71 CC.configure CCconfig @12:54:42.80 CS.reconfigure CSconfig @12:54:42.85 CS.hold @12:54:42.93 CS.configure CSconfig @12:54:43.42 PC.reconfigure PCconfig @12:54:43.47 PC.hold @12:54:43.52 PC.configure PCconfig @12:54:43.61 PS.configure PSconfig @12:54:43.73 Forearm.reconfigure LooseForearm @12:54:43.78 Forearm.hold @12:54:43.83 Forearm.configure LooseForearm @12:54:43.97 Elbow.reconfigure LooseElbow @12:54:44.05 Elbow.hold @12:54:44.17 Elbow.configure LooseElbow @12:54:44.42 SC.reconfigure SCloose @12:54:44.51 SC.hold @12:54:44.62 SC.configure SCloose @12:54:44.82 SE.reconfigure SEconfig @12:54:44.89 SE.hold @12:54:44.94 SE.configure SEconfig @12:54:44.99 CH.coast @12:54:45.04 CH.configure CHconfig @12:54:45.07 PH.coast @12:54:45.13 PH.configure PHconfig @12:54:45.17 PCR.off @12:54:45.20 PCR.pidtemp= 50,0.1,0,300,25,25 @12:54:45.23 PCR.caltemp= 2.8814,0.0372,0 @12:54:45.26 All dwarf controllers running firmware version 3.75 @12:54:45.39 Cleanly exited at 12:52:41CEST24-Jul-20 @12:54:45.43 Loading /home/esp/esp2/mission/stdMERsingleslugs2020.rb mission @12:54:46.06 AFV.select :carrierSolution @12:54:46.16 delay 2 seconds @12:54:47.18 Session Begun @12:54:48.19
AS.seek 0.5ml @12:55:17.31 delay 1 second @12:55:18.33 AFV.select :holdingCoil @12:55:18.45 ATV.dial :extSrc @12:55:19.97 AS.reconfigure ASslow1 @12:55:20.04 AS.seek 0.53ml @12:55:26.39 delay 5 seconds @12:55:31.49 AS.reconfigure ASslow2 @12:55:31.56 AS.seek 0.54ml @12:55:41.90 delay 20 seconds @12:56:01.98 AS.reconfigure ASslow1 @12:56:02.05 AS.seek 0.57ml @12:56:08.34 delay 5 seconds @12:56:13.43 AS.reconfigure ASslow2 @12:56:13.51 AS.seek 0.58ml @12:56:23.81 delay 20 seconds @12:56:43.89 AS.reconfigure ASslow1 @12:56:43.96 AS.seek 0.61ml @12:56:50.26 delay 5 seconds @12:56:55.36 AS.reconfigure ASslow2 @12:56:55.43 AS.seek 0.62ml @12:57:05.78 delay 20 seconds @12:57:25.86 AS.reconfigure ASslow1 @12:57:25.94 AS.seek 0.65ml @12:57:32.28 delay 5 seconds @12:57:37.37 AS.reconfigure ASslow2 @12:57:37.44 AS.seek 0.66ml @12:57:47.72 delay 20 seconds @12:58:07.75 AS.reconfigure ASslow1 @12:58:07.83 AS.seek 0.705ml @12:58:17.17 delay 10 seconds @12:58:27.20 ATV.dial :air1 @12:58:28.95 AS.seek 0.725ml @12:58:33.20 delay 3 seconds @12:58:36.22 ATV.dial :waste @12:58:36.79 AS.reconfigure ASslow0 @12:58:36.86 AS.seek :empty @12:59:50.34 Setting up PCR for ML -- writing /var/log/esp/ML20jul2412h.pcr @12:59:50.37 Building PCR Slug from extSrc with MM2 mix, PP4 primer @12:59:50.41 Priming with carrier @12:59:50.43 ATV.dial ARV @12:59:51.98 ARV.dial :carrier @12:59:52.36 AS.reconfigure ASnorm @12:59:52.43 AS.seek 0.499ml @13:00:17.97 AFV.select :carrierSolution @13:00:18.11 delay 2 seconds @13:00:20.14 AS.reconfigure ASconfig @13:00:20.21 AS.seek 1ml @13:00:49.29 delay 1 second @13:00:50.30 AFV.select :holdingCoil @13:00:50.43 delay 1 second @13:00:51.45 ATV.dial :waste @13:00:53.15 AS.reconfigure ASnorm @13:00:53.23 AS.seek :empty @13:01:44.02 AFV.select :carrierSolution @13:01:44.15 delay 2 seconds @13:01:46.18 AS.reconfigure ASconfig @13:01:46.25 AS.seek 0.5ml @13:02:15.39 delay 1 second @13:02:16.40 AFV.select :holdingCoil @13:02:16.54 Building Slug @13:02:16.55 ATV.dial :ARV @13:02:18.25 delay 2 seconds @13:02:20.27 ARV.dial :air1 @13:02:20.71 delay 2 seconds @13:02:22.79 AS.reconfigure ASslow2 @13:02:22.87 AS.seek 0.506ml @13:02:29.28 delay 2 seconds @13:02:31.31 ARV.dial :MM2 @13:02:32.83 delay 2 seconds @13:02:34.86 Adding MM2 @13:02:34.96 AS.seek 0.524ml @13:02:53.47 delay 25 seconds @13:03:18.49 ARV.dial :PP4 @13:03:18.92 Adding PP4 @13:03:18.94 delay 2 seconds @13:03:21.03 AS.seek 0.53ml @13:03:27.43 delay 10 seconds @13:03:37.45 ARV.dial :air3 @13:03:37.86 adding ARV air @13:03:37.88 delay 2 seconds @13:03:39.97 AS.seek 0.536ml @13:03:46.22 delay 5 seconds @13:03:51.25 ARV.dial :carrier @13:03:52.56 delay 7 seconds @13:03:59.65 AS.seek 0.594ml @13:04:58.20 delay 5 seconds @13:05:03.23 ATV.dial :extSrc @13:05:03.83 delay 2 seconds @13:05:05.92 AS.seek 0.6ml @13:05:12.14 delay 4 seconds @13:05:16.17 ATV.dial ARV @13:05:16.73 delay 2 seconds @13:05:18.76 ARV.dial :carrier @13:05:18.77 delay 2 seconds @13:05:20.88 AS.seek 0.65ml @13:06:11.47 delay 4 seconds @13:06:15.50 ATV.dial PCR @13:06:16.29 ASV.dial :waste1 @13:06:16.40 ARV.dial :air1 @13:06:17.29 Positioning Slug by 0.324ml @13:06:17.41 AS.seek 0.326ml @13:11:43.25 delay 5 seconds @13:11:48.28 ATV.dial ARV @13:11:49.12 ASV.dial :PCRlock @13:11:49.54 delay 5 seconds @13:11:54.57 Running ML Profile60C350 thermocycling @13:11:54.58 PCR.program Profile60C350 @13:11:54.73 Profile60C350 should complete in < 2:02:45 @13:11:54.74 PCR.run 2:38:26.25 @14:24:34.16 -> exit @14:24:34.18 Session Ended @14:52:58.06 PCR.off @14:52:59.35 PCR for ML completed -- closed /var/log/esp/ML20jul2412h.pcr @14:52:59.42 Cleaning PCR @14:52:59.43 AFV.select :carrierSolution @14:52:59.54 delay 2 seconds @14:53:01.57 AS.reconfigure ASconfig @14:53:01.62 AS.seek 0.5ml @14:53:11.96 delay 1 second @14:53:12.97 AFV.select :holdingCoil @14:53:13.08 ASV.dial :waste1 @14:53:13.47 ATV.dial :air3 @14:53:14.77 AS.reconfigure ASslow1 @14:53:14.82 AS.seek 0.51ml @14:53:17.11 delay 2 seconds @14:53:19.12 ATV.dial :bleach @14:53:19.76 AS.seek 0.81ml @14:54:20.35 delay 3 seconds @14:54:23.38 ATV.dial :air3 @14:54:23.93 delay 3 seconds @14:54:27.02 AS.seek 0.835ml @14:54:32.31 delay 3 seconds @14:54:35.33 ATV.dial :bleach @14:54:35.97 AS.seek 0.82ml @14:54:39.22 delay 3 seconds @14:54:42.24 ARV.dial :waste @14:54:42.88 delay 3 seconds @14:54:45.90 ATV.dial :ARV @14:54:46.81 AS.reconfigure ASconfig @14:54:46.86 AS.seek 0.47ml @14:55:07.28 AFV.select :carrierSolution @14:55:07.40 delay 2 seconds @14:55:09.43 AS.seek 1ml @14:55:40.21 delay 1 second @14:55:41.22 AFV.select :holdingCoil @14:55:41.33 delay 1 second @14:55:42.36 AS.reconfigure ASfast @14:55:42.41 AS.seek 0ml @14:56:09.76 AFV.select :carrierSolution @14:56:09.88 delay 2 seconds @14:56:11.91 AS.reconfigure ASconfig @14:56:11.96 AS.seek 1ml @14:57:09.72 delay 1 second @14:57:10.73 AFV.select :holdingCoil @14:57:10.84 delay 1 second @14:57:11.87 AS.reconfigure ASfast @14:57:11.92 AS.seek 0ml @14:57:39.26 AFV.select :carrierSolution @14:57:39.38 delay 2 seconds @14:57:41.41 AS.reconfigure ASconfig @14:57:41.46 AS.seek 1ml @14:58:39.25 delay 1 second @14:58:40.26 AFV.select :holdingCoil @14:58:40.37 delay 1 second @14:58:41.40 AS.reconfigure ASfast @14:58:41.45 AS.seek 0ml @14:59:08.78 ARV.dial between carrier and air1 @14:59:09.30 ATV.dial :air3 @14:59:10.61 AS.reconfigure ASslow1 @14:59:10.67 AS.seek 0.01ml @14:59:13.02 delay 2 seconds @14:59:15.03 ATV.dial :bleach @14:59:15.67 AS.seek 0.81ml @15:01:56.80 delay 3 seconds @15:01:59.81 ATV.dial :air3 @15:02:00.38 delay 3 seconds @15:02:03.47 AS.seek 0.835ml @15:02:08.76 delay 3 seconds @15:02:11.78 ATV.dial :bleach @15:02:12.42 AS.seek 0.82ml @15:02:15.71 delay 3 seconds @15:02:18.72 ATV.dial PCR @15:02:19.14 AS.reconfigure ASconfig @15:02:19.19 AS.seek :empty @15:03:06.67 PCR.program :bleachProfile @15:03:06.73 PCR program should complete in < 2:45 @15:03:06.74 PCR.run 10:00 @15:05:59.88 PCR.off @15:05:59.91 AFV.select :carrierSolution @15:06:00.03 delay 2 seconds @15:06:02.06 AS.seek 1ml @15:06:59.88 delay 1 second @15:07:00.89 AFV.select :holdingCoil @15:07:01.00 delay 1 second @15:07:02.03 AS.reconfigure ASfast @15:07:02.08 AS.seek 0ml @15:07:29.46 delay 3 seconds @15:07:32.48 AFV.select :carrierSolution @15:07:32.59 delay 2 seconds @15:07:34.62 AS.reconfigure ASconfig @15:07:34.68 AS.seek 1ml @15:08:32.44 delay 1 second @15:08:33.45 AFV.select :holdingCoil @15:08:33.56 delay 1 second @15:08:34.59 AS.reconfigure ASfast @15:08:34.64 AS.seek 0ml @15:09:01.99 delay 3 seconds @15:09:05.01 AFV.select :carrierSolution @15:09:05.12 delay 2 seconds @15:09:07.15 AS.reconfigure ASconfig @15:09:07.21 AS.seek 1ml @15:10:05.02 delay 1 second @15:10:06.03 AFV.select :holdingCoil @15:10:06.14 delay 1 second @15:10:07.17 AS.reconfigure ASfast @15:10:07.22 AS.seek 0ml @15:10:34.58 delay 3 seconds @15:10:37.60 AFV.select :carrierSolution @15:10:37.71 delay 2 seconds @15:10:39.74 AS.reconfigure ASconfig @15:10:39.80 AS.seek 1ml @15:11:37.60 delay 1 second @15:11:38.61 AFV.select :holdingCoil @15:11:38.72 delay 1 second @15:11:39.75 AS.reconfigure ASfast @15:11:39.80 AS.seek 0ml @15:12:07.16 delay 3 seconds @15:12:10.18 AFV.select :carrierSolution @15:12:10.29 delay 2 seconds @15:12:12.32 AS.reconfigure ASconfig @15:12:12.38 AS.seek 1ml @15:13:10.17 delay 1 second @15:13:11.18 AFV.select :holdingCoil @15:13:11.29 delay 1 second @15:13:12.32 AS.reconfigure ASnorm @15:13:12.37 AS.seek 0ml @15:14:03.09
AFV.select :carrierSolution @15:14:03.20 delay 2 seconds @15:14:05.22 AS.reconfigure ASconfig @15:14:05.27 AS.seek 0.5ml @15:14:34.35 delay 1 second @15:14:35.36 AFV.select :holdingCoil @15:14:35.47 ATV.dial :air1 @15:14:36.97 AS.reconfigure ASslow2 @15:14:37.02 AS.seek 0.506ml @15:14:43.25 ATV.dial :extSrc @15:14:44.98 AS.seek 0.518ml @15:14:57.21 delay 5 seconds @15:15:02.24 ATV.dial :air1 @15:15:03.98 AS.reconfigure ASslow1 @15:15:04.03 AS.seek 0.548ml @15:15:10.38 ATV.dial :waste @15:15:10.94 AS.reconfigure ASconfig @15:15:10.99 AS.seek :empty @15:15:42.85 AFV.select :carrierSolution @15:15:42.96 delay 2 seconds @15:15:44.98 AS.seek 0.5ml @15:16:14.06 delay 1 second @15:16:15.07 AFV.select :holdingCoil @15:16:15.18 ATV.dial :air1 @15:16:15.80 AS.reconfigure ASslow2 @15:16:15.85 AS.seek 0.51ml @15:16:26.14 delay 5 seconds @15:16:31.16 ATV.dial :extSrc @15:16:32.90 AS.seek 0.53ml @15:16:53.39 delay 10 seconds @15:17:03.42 ATV.dial :air1 @15:17:05.16 AS.reconfigure ASslow1 @15:17:05.21 AS.seek 0.56ml @15:17:11.55 ATV.dial :waste @15:17:12.11 AS.reconfigure ASconfig @15:17:12.16 AS.seek :empty @15:17:44.85 Setting up PCR for ML -- writing /var/log/esp/ML20jul2415h.pcr @15:17:44.88 Building PCR Slug from extSrc with MM2 mix, PP4 primer @15:17:44.89 Priming with carrier @15:17:44.90 ATV.dial ARV @15:17:46.39 ARV.dial :carrier @15:17:46.73 AS.reconfigure ASnorm @15:17:46.78 AS.seek 0.499ml @15:18:12.34 AFV.select :carrierSolution @15:18:12.46 delay 2 seconds @15:18:14.49 AS.reconfigure ASconfig @15:18:14.54 AS.seek 1ml @15:18:43.61 delay 1 second @15:18:44.62 AFV.select :holdingCoil @15:18:44.73 delay 1 second @15:18:45.75 ATV.dial :waste @15:18:47.44 AS.reconfigure ASnorm @15:18:47.49 AS.seek :empty @15:19:38.24 AFV.select :carrierSolution @15:19:38.36 delay 2 seconds @15:19:40.39 AS.reconfigure ASconfig @15:19:40.44 AS.seek 0.5ml @15:20:09.52 delay 1 second @15:20:10.53 AFV.select :holdingCoil @15:20:10.64 Building Slug @15:20:10.65 ATV.dial :ARV @15:20:12.31 delay 2 seconds @15:20:14.33 ARV.dial :air1 @15:20:14.75 delay 2 seconds @15:20:16.83 AS.reconfigure ASslow2 @15:20:16.88 AS.seek 0.506ml @15:20:23.17 delay 2 seconds @15:20:25.18 ARV.dial :MM2 @15:20:26.69 delay 2 seconds @15:20:28.72 Adding MM2 @15:20:28.80 AS.seek 0.524ml @15:20:47.08 delay 25 seconds @15:21:12.10 ARV.dial :PP4 @15:21:12.50 Adding PP4 @15:21:12.51 delay 2 seconds @15:21:14.59 AS.seek 0.53ml @15:21:20.83 delay 10 seconds @15:21:30.85 ARV.dial :air3 @15:21:31.25 adding ARV air @15:21:31.26 delay 2 seconds @15:21:33.35 AS.seek 0.536ml @15:21:39.63 delay 5 seconds @15:21:44.65 ARV.dial :carrier @15:21:45.96 delay 7 seconds @15:21:53.05 AS.seek 0.594ml @15:22:51.69 delay 5 seconds @15:22:56.72 ATV.dial :extSrc @15:22:57.30 delay 2 seconds @15:22:59.39 AS.seek 0.6ml @15:23:06.13 delay 4 seconds @15:23:10.16 ATV.dial ARV @15:23:10.71 delay 2 seconds @15:23:12.74 ARV.dial :carrier @15:23:12.75 delay 2 seconds @15:23:14.84 AS.seek 0.65ml @15:24:05.53 delay 4 seconds @15:24:09.55 ATV.dial PCR @15:24:10.33 ASV.dial :waste1 @15:24:10.34 ARV.dial :air1 @15:24:10.73 Positioning Slug by 0.324ml @15:24:10.82 AS.seek 0.326ml @15:29:36.67 delay 5 seconds @15:29:41.70 ATV.dial ARV @15:29:42.49 ASV.dial :PCRlock @15:29:42.89 delay 5 seconds @15:29:47.92 Running ML Profile60C350 thermocycling @15:29:47.93 PCR.program Profile60C350 @15:29:48.04 Profile60C350 should complete in < 2:02:45 @15:29:48.05 PCR.run 2:38:26.25 @17:10:54.30 PCR.off @17:10:55.59 PCR for ML completed -- closed /var/log/esp/ML20jul2415h.pcr @17:10:55.66 Cleaning PCR @17:10:55.67 AFV.select :carrierSolution @17:10:55.78 delay 2 seconds @17:10:57.81 AS.reconfigure ASconfig @17:10:57.86 AS.seek 0.5ml @17:11:08.17 delay 1 second @17:11:09.18 AFV.select :holdingCoil @17:11:09.29 ASV.dial :waste1 @17:11:09.68 ATV.dial :air3 @17:11:10.99 AS.reconfigure ASslow1 @17:11:11.04 AS.seek 0.51ml @17:11:13.32 delay 2 seconds @17:11:15.34 ATV.dial :bleach @17:11:15.99 AS.seek 0.81ml @17:12:16.59 delay 3 seconds @17:12:19.60 ATV.dial :air3 @17:12:20.17 delay 3 seconds @17:12:23.25 AS.seek 0.835ml @17:12:28.57 delay 3 seconds @17:12:31.59 ATV.dial :bleach @17:12:32.22 AS.seek 0.82ml @17:12:35.52 delay 3 seconds @17:12:38.54 ARV.dial :waste @17:12:39.17 delay 3 seconds @17:12:42.19 ATV.dial :ARV @17:12:43.10 AS.reconfigure ASconfig @17:12:43.15 AS.seek 0.47ml @17:13:03.55 AFV.select :carrierSolution @17:13:03.67 delay 2 seconds @17:13:05.70 AS.seek 1ml @17:13:36.49 delay 1 second @17:13:37.50 AFV.select :holdingCoil @17:13:37.61 delay 1 second @17:13:38.64 AS.reconfigure ASfast @17:13:38.69 AS.seek 0ml @17:14:06.03 AFV.select :carrierSolution @17:14:06.15 delay 2 seconds @17:14:08.18 AS.reconfigure ASconfig @17:14:08.23 AS.seek 1ml @17:15:05.99 delay 1 second @17:15:07.00 AFV.select :holdingCoil @17:15:07.11 delay 1 second @17:15:08.14 AS.reconfigure ASfast @17:15:08.19 AS.seek 0ml @17:15:35.53 AFV.select :carrierSolution @17:15:35.65 delay 2 seconds @17:15:37.68 AS.reconfigure ASconfig @17:15:37.76 AS.seek 1ml @17:16:35.59 delay 1 second @17:16:36.60 AFV.select :holdingCoil @17:16:36.71 delay 1 second @17:16:37.74 AS.reconfigure ASfast @17:16:37.79 AS.seek 0ml @17:17:05.17 ARV.dial between carrier and air1 @17:17:05.71 ATV.dial :air3 @17:17:07.02 AS.reconfigure ASslow1 @17:17:07.07 AS.seek 0.01ml @17:17:09.41 delay 2 seconds @17:17:11.42 ATV.dial :bleach @17:17:12.06 AS.seek 0.81ml @17:19:53.20 delay 3 seconds @17:19:56.22 ATV.dial :air3 @17:19:56.77 delay 3 seconds @17:19:59.85 AS.seek 0.835ml @17:20:05.13 delay 3 seconds @17:20:08.14 ATV.dial :bleach @17:20:09.23 AS.seek 0.82ml @17:20:12.48 delay 3 seconds @17:20:15.49 ATV.dial PCR @17:20:15.91 AS.reconfigure ASconfig @17:20:15.96 AS.seek :empty @17:21:03.39 PCR.program :bleachProfile @17:21:03.46 PCR program should complete in < 2:45 @17:21:03.47 PCR.run 10:00 @17:23:56.20 PCR.off @17:23:56.23 AFV.select :carrierSolution @17:23:56.34 delay 2 seconds @17:23:58.37 AS.seek 1ml @17:24:56.21 delay 1 second @17:24:57.22 AFV.select :holdingCoil @17:24:57.33 delay 1 second @17:24:58.36 AS.reconfigure ASfast @17:24:58.41 AS.seek 0ml @17:25:25.76 delay 3 seconds @17:25:28.78 AFV.select :carrierSolution @17:25:28.89 delay 2 seconds @17:25:30.92 AS.reconfigure ASconfig @17:25:30.97 AS.seek 1ml @17:26:28.73 delay 1 second @17:26:29.74 AFV.select :holdingCoil @17:26:29.85 delay 1 second @17:26:30.88 AS.reconfigure ASfast @17:26:30.93 AS.seek 0ml @17:26:58.29 delay 3 seconds @17:27:01.31 AFV.select :carrierSolution @17:27:01.42 delay 2 seconds @17:27:03.45 AS.reconfigure ASconfig @17:27:03.51 AS.seek 1ml @17:28:01.27 delay 1 second @17:28:02.28 AFV.select :holdingCoil @17:28:02.39 delay 1 second @17:28:03.42 AS.reconfigure ASfast @17:28:03.47 AS.seek 0ml @17:28:30.82 delay 3 seconds @17:28:33.84 AFV.select :carrierSolution @17:28:33.95 delay 2 seconds @17:28:35.98 AS.reconfigure ASconfig @17:28:36.03 AS.seek 1ml @17:29:33.80 delay 1 second @17:29:34.81 AFV.select :holdingCoil @17:29:34.92 delay 1 second @17:29:35.95 AS.reconfigure ASfast @17:29:36.00 AS.seek 0ml @17:30:03.35 delay 3 seconds @17:30:06.37 AFV.select :carrierSolution @17:30:06.48 delay 2 seconds @17:30:08.51 AS.reconfigure ASconfig @17:30:08.57 AS.seek 1ml @17:31:06.37 delay 1 second @17:31:07.38 AFV.select :holdingCoil @17:31:07.49 delay 1 second @17:31:08.52 AS.reconfigure ASnorm @17:31:08.57 AS.seek 0ml @17:31:59.30
AFV.select :carrierSolution @17:31:59.41 delay 2 seconds @17:32:01.43 AS.reconfigure ASconfig @17:32:01.48 AS.seek 0.5ml @17:32:30.54 delay 1 second @17:32:31.55 AFV.select :holdingCoil @17:32:31.66 ATV.dial :air1 @17:32:33.16 AS.reconfigure ASslow2 @17:32:33.21 AS.seek 0.506ml @17:32:39.51 ATV.dial :extSrc @17:32:41.23 AS.seek 0.518ml @17:32:53.57 delay 5 seconds @17:32:58.60 ATV.dial :air1 @17:33:00.38 AS.reconfigure ASslow1 @17:33:00.43 AS.seek 0.548ml @17:33:06.76 ATV.dial :waste @17:33:07.32 AS.reconfigure ASconfig @17:33:07.37 AS.seek :empty @17:33:39.23 AFV.select :carrierSolution @17:33:39.34 delay 2 seconds @17:33:41.36 AS.seek 0.5ml @17:34:10.48 delay 1 second @17:34:11.49 AFV.select :holdingCoil @17:34:11.60 ATV.dial :air1 @17:34:12.21 AS.reconfigure ASslow1 @17:34:12.26 AS.seek 0.52ml @17:34:16.55 delay 5 seconds @17:34:21.58 ATV.dial :extSrc @17:34:23.32 AS.reconfigure ASslow2 @17:34:23.37 AS.seek 0.545ml @17:34:48.76 delay 10 seconds @17:34:58.79 ATV.dial :air1 @17:35:00.52 AS.reconfigure ASslow1 @17:35:00.57 AS.seek 0.575ml @17:35:06.90 ATV.dial :waste @17:35:07.44 AS.reconfigure ASconfig @17:35:07.49 AS.seek :empty @17:35:41.04 Setting up PCR for ML -- writing /var/log/esp/ML20jul2417h.pcr @17:35:41.07 Building PCR Slug from extSrc with MM2 mix, PP4 primer @17:35:41.08 Priming with carrier @17:35:41.09 ATV.dial ARV @17:35:42.60 ARV.dial :carrier @17:35:42.95 AS.reconfigure ASnorm @17:35:43.00 AS.seek 0.499ml @17:36:08.62 AFV.select :carrierSolution @17:36:08.74 delay 2 seconds @17:36:10.77 AS.reconfigure ASconfig @17:36:10.82 AS.seek 1ml @17:36:39.90 delay 1 second @17:36:40.91 AFV.select :holdingCoil @17:36:41.02 delay 1 second @17:36:42.05 ATV.dial :waste @17:36:43.73 AS.reconfigure ASnorm @17:36:43.78 AS.seek :empty @17:37:34.53 AFV.select :carrierSolution @17:37:34.65 delay 2 seconds @17:37:36.68 AS.reconfigure ASconfig @17:37:36.73 AS.seek 0.5ml @17:38:06.24 delay 1 second @17:38:07.25 AFV.select :holdingCoil @17:38:07.36 Building Slug @17:38:07.37 ATV.dial :ARV @17:38:09.02 delay 2 seconds @17:38:11.05 ARV.dial :air1 @17:38:11.55 delay 2 seconds @17:38:13.63 AS.reconfigure ASslow2 @17:38:13.69 AS.seek 0.506ml @17:38:20.01 delay 2 seconds @17:38:22.03 ARV.dial :MM2 @17:38:23.55 delay 2 seconds @17:38:25.58 Adding MM2 @17:38:25.66 AS.seek 0.524ml @17:38:44.18 delay 25 seconds @17:39:09.21 ARV.dial :PP4 @17:39:09.60 Adding PP4 @17:39:09.61 delay 2 seconds @17:39:11.70 AS.seek 0.53ml @17:39:18.04 delay 10 seconds @17:39:28.07 ARV.dial :air3 @17:39:28.46 adding ARV air @17:39:28.47 delay 2 seconds @17:39:30.56 AS.seek 0.536ml @17:39:37.04 delay 5 seconds @17:39:42.07 ARV.dial :carrier @17:39:43.35 delay 7 seconds @17:39:50.44 AS.seek 0.594ml @17:40:49.05 delay 5 seconds @17:40:54.08 ATV.dial :extSrc @17:40:54.65 delay 2 seconds @17:40:56.74 AS.seek 0.6ml @17:41:03.02 delay 4 seconds @17:41:07.05 ATV.dial ARV @17:41:07.60 delay 2 seconds @17:41:09.63 ARV.dial :carrier @17:41:09.64 delay 2 seconds @17:41:11.73 AS.seek 0.65ml @17:42:02.54 delay 4 seconds @17:42:06.56 ATV.dial PCR @17:42:07.33 ASV.dial :waste1 @17:42:07.34 ARV.dial :air1 @17:42:07.76 Positioning Slug by 0.324ml @17:42:07.85 AS.seek 0.326ml @17:47:33.85 delay 5 seconds @17:47:38.88 ATV.dial ARV @17:47:39.66 ASV.dial :PCRlock @17:47:40.05 delay 5 seconds @17:47:45.08 Running ML Profile60C350 thermocycling @17:47:45.09 PCR.program Profile60C350 @17:47:45.20 Profile60C350 should complete in < 2:02:45 @17:47:45.21 PCR.run 2:38:26.25 @19:28:53.20 PCR.off @19:28:54.51 PCR for ML completed -- closed /var/log/esp/ML20jul2417h.pcr @19:28:54.58 Cleaning PCR @19:28:54.59 AFV.select :carrierSolution @19:28:54.70 delay 2 seconds @19:28:56.73 AS.reconfigure ASconfig @19:28:56.78 AS.seek 0.5ml @19:29:07.09 delay 1 second @19:29:08.10 AFV.select :holdingCoil @19:29:08.21 ASV.dial :waste1 @19:29:08.63 ATV.dial :air3 @19:29:10.40 AS.reconfigure ASslow1 @19:29:10.46 AS.seek 0.51ml @19:29:12.73 delay 2 seconds @19:29:14.75 ATV.dial :bleach @19:29:15.40 AS.seek 0.81ml @19:30:16.00 delay 3 seconds @19:30:19.02 ATV.dial :air3 @19:30:19.58 delay 3 seconds @19:30:22.67 AS.seek 0.835ml @19:30:27.97 delay 3 seconds @19:30:31.00 ATV.dial :bleach @19:30:31.62 AS.seek 0.82ml @19:30:34.92 delay 3 seconds @19:30:37.95 ARV.dial :waste @19:30:38.56 delay 3 seconds @19:30:41.58 ATV.dial :ARV @19:30:42.48 AS.reconfigure ASconfig @19:30:42.54 AS.seek 0.47ml @19:31:02.94 AFV.select :carrierSolution @19:31:03.06 delay 2 seconds @19:31:05.09 AS.seek 1ml @19:31:35.84 delay 1 second @19:31:36.85 AFV.select :holdingCoil @19:31:36.96 delay 1 second @19:31:37.99 AS.reconfigure ASfast @19:31:38.04 AS.seek 0ml @19:32:05.39 AFV.select :carrierSolution @19:32:05.50 delay 2 seconds @19:32:07.53 AS.reconfigure ASconfig @19:32:07.58 AS.seek 1ml @19:33:05.33 delay 1 second @19:33:06.34 AFV.select :holdingCoil @19:33:06.45 delay 1 second @19:33:07.48 AS.reconfigure ASfast @19:33:07.53 AS.seek 0ml @19:33:34.89 AFV.select :carrierSolution @19:33:35.01 delay 2 seconds @19:33:37.04 AS.reconfigure ASconfig @19:33:37.09 AS.seek 1ml @19:34:34.86 delay 1 second @19:34:35.87 AFV.select :holdingCoil @19:34:35.98 delay 1 second @19:34:37.01 AS.reconfigure ASfast @19:34:37.06 AS.seek 0ml @19:35:04.44 ARV.dial between carrier and air1 @19:35:04.95 ATV.dial :air3 @19:35:06.26 AS.reconfigure ASslow1 @19:35:06.31 AS.seek 0.01ml @19:35:08.61 delay 2 seconds @19:35:10.62 ATV.dial :bleach @19:35:11.26 AS.seek 0.81ml @19:37:52.39 delay 3 seconds @19:37:55.41 ATV.dial :air3 @19:37:55.97 delay 3 seconds @19:37:59.05 AS.seek 0.835ml @19:38:04.35 delay 3 seconds @19:38:07.37 ATV.dial :bleach @19:38:08.01 AS.seek 0.82ml @19:38:11.33 delay 3 seconds @19:38:14.35 ATV.dial PCR @19:38:14.76 AS.reconfigure ASconfig @19:38:14.81 AS.seek :empty @19:39:02.27 PCR.program :bleachProfile @19:39:02.34 PCR program should complete in < 2:45 @19:39:02.35 PCR.run 10:00 @19:41:55.09 PCR.off @19:41:55.11 AFV.select :carrierSolution @19:41:55.22 delay 2 seconds @19:41:57.25 AS.seek 1ml @19:42:55.06 delay 1 second @19:42:56.07 AFV.select :holdingCoil @19:42:56.18 delay 1 second @19:42:57.21 AS.reconfigure ASfast @19:42:57.26 AS.seek 0ml @19:43:24.72 delay 3 seconds @19:43:27.73 AFV.select :carrierSolution @19:43:27.84 delay 2 seconds @19:43:29.87 AS.reconfigure ASconfig @19:43:29.92 AS.seek 1ml @19:44:27.72 delay 1 second @19:44:28.73 AFV.select :holdingCoil @19:44:28.84 delay 1 second @19:44:29.87 AS.reconfigure ASfast @19:44:29.92 AS.seek 0ml @19:44:57.28 delay 3 seconds @19:45:00.30 AFV.select :carrierSolution @19:45:00.41 delay 2 seconds @19:45:02.44 AS.reconfigure ASconfig @19:45:02.50 AS.seek 1ml @19:46:00.25 delay 1 second @19:46:01.26 AFV.select :holdingCoil @19:46:01.37 delay 1 second @19:46:02.40 AS.reconfigure ASfast @19:46:02.45 AS.seek 0ml @19:46:29.79 delay 3 seconds @19:46:32.80 AFV.select :carrierSolution @19:46:32.91 delay 2 seconds @19:46:34.94 AS.reconfigure ASconfig @19:46:34.99 AS.seek 1ml @19:47:33.23 delay 1 second @19:47:34.24 AFV.select :holdingCoil @19:47:34.35 delay 1 second @19:47:35.38 AS.reconfigure ASfast @19:47:35.43 AS.seek 0ml @19:48:02.87 delay 3 seconds @19:48:05.89 AFV.select :carrierSolution @19:48:06.00 delay 2 seconds @19:48:08.03 AS.reconfigure ASconfig @19:48:08.08 AS.seek 1ml @19:49:05.87 delay 1 second @19:49:06.88 AFV.select :holdingCoil @19:49:06.99 delay 1 second @19:49:08.02 AS.reconfigure ASnorm @19:49:08.07 AS.seek 0ml @19:49:58.79
AFV.select :carrierSolution @19:49:58.90 delay 2 seconds @19:50:00.92 AS.reconfigure ASconfig @19:50:00.97 AS.seek 0.5ml @19:50:30.04 delay 1 second @19:50:31.05 AFV.select :holdingCoil @19:50:31.16 ATV.dial :air1 @19:50:32.67 AS.reconfigure ASslow2 @19:50:32.72 AS.seek 0.506ml @19:50:39.20 ATV.dial :extSrc @19:50:40.91 AS.seek 0.518ml @19:50:53.32 delay 5 seconds @19:50:58.35 ATV.dial :air1 @19:51:00.09 AS.reconfigure ASslow1 @19:51:00.14 AS.seek 0.548ml @19:51:06.43 ATV.dial :waste @19:51:06.98 AS.reconfigure ASconfig @19:51:07.03 AS.seek :empty @19:51:38.90 AFV.select :carrierSolution @19:51:39.01 delay 2 seconds @19:51:41.04 AS.seek 0.5ml @19:52:10.15 delay 1 second @19:52:11.16 AFV.select :holdingCoil @19:52:11.27 ATV.dial :air1 @19:52:11.88 AS.reconfigure ASslow1 @19:52:11.93 AS.seek 0.52ml @19:52:16.32 delay 5 seconds @19:52:21.35 ATV.dial :extSrc @19:52:23.07 AS.reconfigure ASslow2 @19:52:23.12 AS.seek 0.538ml @19:52:41.45 delay 10 seconds @19:52:51.48 ATV.dial :air1 @19:52:53.20 AS.reconfigure ASslow1 @19:52:53.25 AS.seek 0.568ml @19:52:59.54 ATV.dial :waste @19:53:00.09 AS.reconfigure ASconfig @19:53:00.14 AS.seek :empty @19:53:33.33 Setting up PCR for ML -- writing /var/log/esp/ML20jul2419h.pcr @19:53:33.36 Building PCR Slug from extSrc with MM2 mix, PP4 primer @19:53:33.37 Priming with carrier @19:53:33.38 ATV.dial ARV @19:53:34.87 ARV.dial :carrier @19:53:35.21 AS.reconfigure ASnorm @19:53:35.27 AS.seek 0.499ml @19:54:00.89 AFV.select :carrierSolution @19:54:01.01 delay 2 seconds @19:54:03.04 AS.reconfigure ASconfig @19:54:03.09 AS.seek 1ml @19:54:32.15 delay 1 second @19:54:33.16 AFV.select :holdingCoil @19:54:33.27 delay 1 second @19:54:34.30 ATV.dial :waste @19:54:35.98 AS.reconfigure ASnorm @19:54:36.03 AS.seek :empty @19:55:26.77 AFV.select :carrierSolution @19:55:26.89 delay 2 seconds @19:55:28.92 AS.reconfigure ASconfig @19:55:28.97 AS.seek 0.5ml @19:55:58.07 delay 1 second @19:55:59.08 AFV.select :holdingCoil @19:55:59.19 Building Slug @19:55:59.20 ATV.dial :ARV @19:56:00.86 delay 2 seconds @19:56:02.89 ARV.dial :air1 @19:56:03.29 delay 2 seconds @19:56:05.37 AS.reconfigure ASslow2 @19:56:05.42 AS.seek 0.506ml @19:56:11.81 delay 2 seconds @19:56:13.83 ARV.dial :MM2 @19:56:15.36 delay 2 seconds @19:56:17.39 Adding MM2 @19:56:17.46 AS.seek 0.524ml @19:56:35.79 delay 25 seconds @19:57:00.82 ARV.dial :PP4 @19:57:01.21 Adding PP4 @19:57:01.22 delay 2 seconds @19:57:03.31 AS.seek 0.53ml @19:57:09.67 delay 10 seconds @19:57:19.70 ARV.dial :air3 @19:57:20.09 adding ARV air @19:57:20.10 delay 2 seconds @19:57:22.19 AS.seek 0.536ml @19:57:28.68 delay 5 seconds @19:57:33.70 ARV.dial :carrier @19:57:35.01 delay 7 seconds @19:57:42.10 AS.seek 0.594ml @19:58:40.79 delay 5 seconds @19:58:45.82 ATV.dial :extSrc @19:58:46.82 delay 2 seconds @19:58:48.91 AS.seek 0.6ml @19:58:55.29 delay 4 seconds @19:58:59.32 ATV.dial ARV @19:58:59.87 delay 2 seconds @19:59:01.90 ARV.dial :carrier @19:59:01.91 delay 2 seconds @19:59:04.00 AS.seek 0.65ml @19:59:54.70 delay 4 seconds @19:59:58.73 ATV.dial PCR @19:59:59.50 ASV.dial :waste1 @19:59:59.51 ARV.dial :air1 @19:59:59.90 Positioning Slug by 0.324ml @19:59:59.99 AS.seek 0.326ml @20:05:25.95 delay 5 seconds @20:05:30.98 ATV.dial ARV @20:05:31.76 ASV.dial :PCRlock @20:05:32.17 delay 5 seconds @20:05:37.20 Running ML Profile60C350 thermocycling @20:05:37.21 PCR.program Profile60C350 @20:05:37.32 Profile60C350 should complete in < 2:02:45 @20:05:37.34 PCR.run 2:38:26.25 @21:46:11.51 PCR.off @21:46:12.82 PCR for ML completed -- closed /var/log/esp/ML20jul2419h.pcr @21:46:12.89 Cleaning PCR @21:46:12.91 AFV.select :carrierSolution @21:46:13.02 delay 2 seconds @21:46:15.05 AS.reconfigure ASconfig @21:46:15.10 AS.seek 0.5ml @21:46:25.39 delay 1 second @21:46:26.40 AFV.select :holdingCoil @21:46:26.51 ASV.dial :waste1 @21:46:26.91 ATV.dial :air3 @21:46:28.22 AS.reconfigure ASslow1 @21:46:28.27 AS.seek 0.51ml @21:46:30.55 delay 2 seconds @21:46:32.57 ATV.dial :bleach @21:46:33.21 AS.seek 0.81ml @21:47:33.80 delay 3 seconds @21:47:36.83 ATV.dial :air3 @21:47:37.38 delay 3 seconds @21:47:40.46 AS.seek 0.835ml @21:47:45.78 delay 3 seconds @21:47:48.81 ATV.dial :bleach @21:47:49.43 AS.seek 0.82ml @21:47:52.80 delay 3 seconds @21:47:55.82 ARV.dial :waste @21:47:56.44 delay 3 seconds @21:47:59.46 ATV.dial :ARV @21:48:00.47 AS.reconfigure ASconfig @21:48:00.52 AS.seek 0.47ml @21:48:20.95 AFV.select :carrierSolution @21:48:21.07 delay 2 seconds @21:48:23.10 AS.seek 1ml @21:48:53.83 delay 1 second @21:48:54.84 AFV.select :holdingCoil @21:48:54.95 delay 1 second @21:48:55.98 AS.reconfigure ASfast @21:48:56.03 AS.seek 0ml @21:49:23.37 AFV.select :carrierSolution @21:49:23.49 delay 2 seconds @21:49:25.52 AS.reconfigure ASconfig @21:49:25.57 AS.seek 1ml @21:50:23.36 delay 1 second @21:50:24.38 AFV.select :holdingCoil @21:50:24.49 delay 1 second @21:50:25.52 AS.reconfigure ASfast @21:50:25.57 AS.seek 0ml @21:50:52.91 AFV.select :carrierSolution @21:50:53.03 delay 2 seconds @21:50:55.51 AS.reconfigure ASconfig @21:50:55.56 AS.seek 1ml @21:51:53.30 delay 1 second @21:51:54.31 AFV.select :holdingCoil @21:51:54.42 delay 1 second @21:51:55.45 AS.reconfigure ASfast @21:51:55.50 AS.seek 0ml @21:52:22.84 ARV.dial between carrier and air1 @21:52:23.36 ATV.dial :air3 @21:52:24.67 AS.reconfigure ASslow1 @21:52:24.72 AS.seek 0.01ml @21:52:27.07 delay 2 seconds @21:52:29.08 ATV.dial :bleach @21:52:29.71 AS.seek 0.81ml @21:55:10.86 delay 3 seconds @21:55:13.88 ATV.dial :air3 @21:55:14.44 delay 3 seconds @21:55:17.53 AS.seek 0.835ml @21:55:22.82 delay 3 seconds @21:55:25.85 ATV.dial :bleach @21:55:26.48 AS.seek 0.82ml @21:55:29.78 delay 3 seconds @21:55:32.80 ATV.dial PCR @21:55:33.22 AS.reconfigure ASconfig @21:55:33.27 AS.seek :empty @21:56:20.74 PCR.program :bleachProfile @21:56:20.81 PCR program should complete in < 2:45 @21:56:20.82 PCR.run 10:00 @21:59:13.55 PCR.off @21:59:13.58 AFV.select :carrierSolution @21:59:13.69 delay 2 seconds @21:59:15.72 AS.seek 1ml @22:00:13.56 delay 1 second @22:00:14.57 AFV.select :holdingCoil @22:00:14.68 delay 1 second @22:00:15.71 AS.reconfigure ASfast @22:00:15.76 AS.seek 0ml @22:00:43.10 delay 3 seconds @22:00:46.12 AFV.select :carrierSolution @22:00:46.23 delay 2 seconds @22:00:48.26 AS.reconfigure ASconfig @22:00:48.31 AS.seek 1ml @22:01:46.05 delay 1 second @22:01:47.06 AFV.select :holdingCoil @22:01:47.17 delay 1 second @22:01:48.20 AS.reconfigure ASfast @22:01:48.25 AS.seek 0ml @22:02:15.61 delay 3 seconds @22:02:18.63 AFV.select :carrierSolution @22:02:18.74 delay 2 seconds @22:02:20.77 AS.reconfigure ASconfig @22:02:20.83 AS.seek 1ml @22:03:18.58 delay 1 second @22:03:19.59 AFV.select :holdingCoil @22:03:19.70 delay 1 second @22:03:20.73 AS.reconfigure ASfast @22:03:20.78 AS.seek 0ml @22:03:48.14 delay 3 seconds @22:03:51.16 AFV.select :carrierSolution @22:03:51.27 delay 2 seconds @22:03:53.30 AS.reconfigure ASconfig @22:03:53.35 AS.seek 1ml @22:04:51.14 delay 1 second @22:04:52.15 AFV.select :holdingCoil @22:04:52.26 delay 1 second @22:04:53.29 AS.reconfigure ASfast @22:04:53.34 AS.seek 0ml @22:05:20.71 delay 3 seconds @22:05:23.73 AFV.select :carrierSolution @22:05:23.84 delay 2 seconds @22:05:25.87 AS.reconfigure ASconfig @22:05:25.92 AS.seek 1ml @22:06:23.69 delay 1 second @22:06:24.70 AFV.select :holdingCoil @22:06:24.81 delay 1 second @22:06:25.84 AS.reconfigure ASnorm @22:06:25.89 AS.seek 0ml @22:07:16.62
AFV.select :carrierSolution @22:07:16.73 delay 2 seconds @22:07:18.75 AS.reconfigure ASconfig @22:07:18.80 AS.seek 0.5ml @22:07:47.86 delay 1 second @22:07:48.87 AFV.select :holdingCoil @22:07:48.98 ATV.dial :air1 @22:07:50.48 AS.reconfigure ASslow2 @22:07:50.53 AS.seek 0.506ml @22:07:56.74 ATV.dial :extSrc @22:07:58.45 AS.seek 0.518ml @22:08:10.67 delay 5 seconds @22:08:15.70 ATV.dial :air1 @22:08:17.44 AS.reconfigure ASslow1 @22:08:17.49 AS.seek 0.548ml @22:08:23.81 ATV.dial :waste @22:08:24.36 AS.reconfigure ASconfig @22:08:24.41 AS.seek :empty @22:08:56.28 deconEluateRecoveryLine extSrc @22:08:56.29 ATV.dial :waste @22:08:56.30 AS.reconfigure ASnorm @22:08:56.35 AS.seek 0ml @22:08:56.76 ATV.dial :air3 @22:08:57.45 AS.reconfigure ASslow1 @22:08:57.50 AS.seek 0.01ml @22:09:00.27 delay 2 seconds @22:09:02.28 ATV.dial :bleach @22:09:02.76 AS.seek 0.31ml @22:10:03.41 delay 3 seconds @22:10:06.42 ATV.dial :air3 @22:10:06.99 delay 3 seconds @22:10:10.07 AS.seek 0.335ml @22:10:15.37 delay 3 seconds @22:10:18.38 ATV.dial :bleach @22:10:19.01 AS.seek 0.32ml @22:10:22.33 delay 3 seconds @22:10:25.35 ATV.dial :extSrc @22:10:25.98 AS.seek 0ml @22:11:30.58 AFV.select :carrierSolution @22:11:30.70 delay 2 seconds @22:11:32.72 AS.reconfigure ASconfig @22:11:32.77 AS.seek 0.5ml @22:12:01.81 delay 1 second @22:12:02.82 AFV.select :holdingCoil @22:12:02.93 ATV.dial :extSrc @22:12:02.95 AS.reconfigure ASslow1 @22:12:02.99 AS.seek 0ml @22:13:43.77 AFV.select :carrierSolution @22:13:43.89 delay 2 seconds @22:13:45.91 AS.reconfigure ASconfig @22:13:45.96 AS.seek 0.5ml @22:14:14.97 delay 1 second @22:14:15.98 AFV.select :holdingCoil @22:14:16.09 ATV.dial :extSrc @22:14:16.11 AS.reconfigure ASslow1 @22:14:16.16 AS.seek 0ml @22:15:56.99 AFV.select :carrierSolution @22:15:57.11 delay 2 seconds @22:15:59.13 AS.reconfigure ASconfig @22:15:59.18 AS.seek 0.5ml @22:16:28.22 delay 1 second @22:16:29.23 AFV.select :holdingCoil @22:16:29.34 ATV.dial :extSrc @22:16:29.36 AS.reconfigure ASslow1 @22:16:29.41 AS.seek 0ml @22:18:10.24 AFV.select :carrierSolution @22:18:10.35 delay 2 seconds @22:18:12.37 AS.reconfigure ASconfig @22:18:12.42 AS.seek 0.5ml @22:18:41.44 delay 1 second @22:18:42.45 AFV.select :holdingCoil @22:18:42.56 ATV.dial :extSrc @22:18:42.58 AS.reconfigure ASslow1 @22:18:42.63 AS.seek 0ml @22:20:23.46 ATV.dial :air2 @22:20:24.82 AS.reconfigure ASslow0 @22:20:24.86 AS.seek 0.5ml @22:21:15.49 ATV.dial :extSrc @22:21:16.99 AS.seek 0.02ml @22:22:05.59 delay 1 second @22:22:06.61 ATV.dial :waste @22:22:07.91 AS.reconfigure ASconfig @22:22:07.96 AS.seek :empty @22:22:09.47 deconEluateRecoveryLine extSrc @22:22:09.48 ATV.dial :waste @22:22:09.49 AS.reconfigure ASnorm @22:22:09.54 AS.seek 0ml @22:22:09.93 ATV.dial :air3 @22:22:10.78 AS.reconfigure ASslow1 @22:22:10.83 AS.seek 0.01ml @22:22:13.08 delay 2 seconds @22:22:15.09 ATV.dial :bleach @22:22:15.59 AS.seek 0.31ml @22:23:16.19 delay 3 seconds @22:23:19.21 ATV.dial :air3 @22:23:19.78 delay 3 seconds @22:23:22.86 AS.seek 0.335ml @22:23:28.20 delay 3 seconds @22:23:31.21 ATV.dial :bleach @22:23:31.85 AS.seek 0.32ml @22:23:35.13 delay 3 seconds @22:23:38.15 ATV.dial :extSrc @22:23:38.77 AS.seek 0ml @22:24:43.37 AFV.select :carrierSolution @22:24:43.48 delay 2 seconds @22:24:45.50 AS.reconfigure ASconfig @22:24:45.55 AS.seek 0.5ml @22:25:14.59 delay 1 second @22:25:15.60 AFV.select :holdingCoil @22:25:15.71 ATV.dial :extSrc @22:25:15.72 AS.reconfigure ASslow1 @22:25:15.78 AS.seek 0ml @22:26:56.57 AFV.select :carrierSolution @22:26:56.69 delay 2 seconds @22:26:58.71 AS.reconfigure ASconfig @22:26:58.76 AS.seek 0.5ml @22:27:27.80 delay 1 second @22:27:28.81 AFV.select :holdingCoil @22:27:28.92 ATV.dial :extSrc @22:27:28.94 AS.reconfigure ASslow1 @22:27:28.99 AS.seek 0ml @22:29:09.82 AFV.select :carrierSolution @22:29:09.94 delay 2 seconds @22:29:11.96 AS.reconfigure ASconfig @22:29:12.01 AS.seek 0.5ml @22:29:41.05 delay 1 second @22:29:42.06 AFV.select :holdingCoil @22:29:42.17 ATV.dial :extSrc @22:29:42.18 AS.reconfigure ASslow1 @22:29:42.24 AS.seek 0ml @22:31:23.02 AFV.select :carrierSolution @22:31:23.14 delay 2 seconds @22:31:25.16 AS.reconfigure ASconfig @22:31:25.21 AS.seek 0.5ml @22:31:54.27 delay 1 second @22:31:55.28 AFV.select :holdingCoil @22:31:55.39 ATV.dial :extSrc @22:31:55.41 AS.reconfigure ASslow1 @22:31:55.46 AS.seek 0ml @22:33:36.27 ATV.dial :air2 @22:33:38.04 AS.reconfigure ASslow0 @22:33:38.09 AS.seek 0.5ml @22:34:28.72 ATV.dial :extSrc @22:34:30.23 AS.seek 0.02ml @22:35:18.83 delay 1 second @22:35:19.85 ATV.dial :waste @22:35:21.16 AS.reconfigure ASconfig @22:35:21.21 AS.seek :empty @22:35:22.72 deconEluateRecoveryLine extSrc @22:35:22.73 ATV.dial :waste @22:35:22.75 AS.reconfigure ASnorm @22:35:22.79 AS.seek 0ml @22:35:23.18 ATV.dial :air3 @22:35:24.04 AS.reconfigure ASslow1 @22:35:24.09 AS.seek 0.01ml @22:35:26.34 delay 2 seconds @22:35:28.35 ATV.dial :bleach @22:35:28.84 AS.seek 0.31ml @22:36:29.42 delay 3 seconds @22:36:32.43 ATV.dial :air3 @22:36:33.00 delay 3 seconds @22:36:36.08 AS.seek 0.335ml @22:36:41.42 delay 3 seconds @22:36:44.43 ATV.dial :bleach @22:36:45.07 AS.seek 0.32ml @22:36:48.39 delay 3 seconds @22:36:51.40 ATV.dial :extSrc @22:36:52.03 AS.seek 0ml @22:37:56.65 AFV.select :carrierSolution @22:37:56.76 delay 2 seconds @22:37:58.78 AS.reconfigure ASconfig @22:37:58.83 AS.seek 0.5ml @22:38:27.86 delay 1 second @22:38:28.87 AFV.select :holdingCoil @22:38:28.98 ATV.dial :extSrc @22:38:29.00 AS.reconfigure ASslow1 @22:38:29.05 AS.seek 0ml @22:40:09.86 AFV.select :carrierSolution @22:40:09.98 delay 2 seconds @22:40:12.00 AS.reconfigure ASconfig @22:40:12.05 AS.seek 0.5ml @22:40:41.08 delay 1 second @22:40:42.09 AFV.select :holdingCoil @22:40:42.20 ATV.dial :extSrc @22:40:42.22 AS.reconfigure ASslow1 @22:40:42.27 AS.seek 0ml @22:42:23.08 AFV.select :carrierSolution @22:42:23.19 delay 2 seconds @22:42:25.21 AS.reconfigure ASconfig @22:42:25.26 AS.seek 0.5ml @22:42:54.28 delay 1 second @22:42:55.29 AFV.select :holdingCoil @22:42:55.40 ATV.dial :extSrc @22:42:55.42 AS.reconfigure ASslow1 @22:42:55.47 AS.seek 0ml @22:44:36.29 AFV.select :carrierSolution @22:44:36.41 delay 2 seconds @22:44:38.43 AS.reconfigure ASconfig @22:44:38.48 AS.seek 0.5ml @22:45:07.48 delay 1 second @22:45:08.49 AFV.select :holdingCoil @22:45:08.60 ATV.dial :extSrc @22:45:08.62 AS.reconfigure ASslow1 @22:45:08.67 AS.seek 0ml @22:46:49.46 ATV.dial :air2 @22:46:50.82 AS.reconfigure ASslow0 @22:46:50.87 AS.seek 0.5ml @22:47:41.52 ATV.dial :extSrc @22:47:43.02 AS.seek 0.02ml @22:48:31.62 delay 1 second @22:48:32.64 ATV.dial :waste @22:48:33.94 AS.reconfigure ASconfig @22:48:33.99 AS.seek :empty @16:47:55.02CEST27-Jul-20
ESP2 v3.30 I2C Gateway v3.06 for core bus @16:47:57.02 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @16:47:57.34 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @16:48:01.41 LoadError in MAIN -- no such file to load -- shortmfb in MFB @16:48:02.36 Polling Can every 10:00 @16:48:04.19 SPE.coast @16:48:04.25 SPE.configure SPEconfig @16:48:04.35 AS.reconfigure ASconfig @16:48:04.39 AS.hold @16:48:04.45 AS.configure ASconfig @16:48:04.55 SS.reconfigure SSconfig @16:48:04.59 SS.hold @16:48:04.65 SS.configure SSconfig @16:48:04.74 CC.reconfigure CCconfig @16:48:04.78 CC.hold @16:48:04.86 CC.configure CCconfig @16:48:04.96 CS.reconfigure CSconfig @16:48:05.00 CS.hold @16:48:05.08 CS.configure CSconfig @16:48:05.18 PC.reconfigure PCconfig @16:48:05.22 PC.hold @16:48:05.27 PC.configure PCconfig @16:48:05.37 PS.configure PSconfig @16:48:05.49 Forearm.reconfigure LooseForearm @16:48:05.53 Forearm.hold @16:48:05.59 Forearm.configure LooseForearm @16:48:05.68 Elbow.reconfigure LooseElbow @16:48:05.73 Elbow.hold @16:48:05.79 Elbow.configure LooseElbow @16:48:05.91 SC.reconfigure SCloose @16:48:05.95 SC.hold @16:48:06.01 SC.configure SCloose @16:48:06.11 SE.reconfigure SEconfig @16:48:06.16 SE.hold @16:48:06.22 SE.configure SEconfig @16:48:06.26 CH.coast @16:48:06.31 CH.configure CHconfig @16:48:06.34 PH.coast @16:48:06.40 PH.configure PHconfig @16:48:06.44 PCR.off @16:48:06.47 PCR.pidtemp= 50,0.1,0,300,25,25 @16:48:06.50 PCR.caltemp= 2.8814,0.0372,0 @16:48:06.54 All dwarf controllers running firmware version 3.75 @16:48:07.09 Cleanly exited at 16:42:35CEST27-Jul-20 @16:48:07.13 Loading /home/esp/esp2/mission/stdMERsingleslugs2020.rb mission @16:48:07.69 NoMethodError in MAIN -- undefined method `ready!' for MFB:Module @16:48:07.71 Waiting to Resume from Checkpoint "Loading /home/esp/esp2/mission/stdMERsingleslugs2020.rb mission" @16:48:07.72 BadNews.email "undefined method `ready!' for MFB:Module",Subject: "NoMethodError in MAIN" @16:48:10.32 Session Begun @16:49:49.40 -> Abort! "error in script I guess" @16:49:49.42 NoMethodError in mwj -- undefined method `Abort!' for # @16:49:57.44 -> abort! "error in script I guess" @16:49:57.45 CmdServer::Client::Error in mwj -- Aborting Mission: error in script I guess @16:49:57.49 BadNews.email "error in script I guess",Subject: "Mission ABORTED!" @16:50:04.49 -> exit @16:50:04.52 Session Ended @14:06:54.31CEST29-Jul-20
ESP2 v3.30 I2C Gateway v3.06 for core bus @14:06:56.28 Copyright 2018 MBARI /home/esp/esp2/type/shallow/mer/configure.rb @14:06:56.60 Configuration for MFB3 by preston@mbari.org /home/esp/esp2/type/mfb/MFB3/configure.rb @14:07:02.02 Polling Can every 10:00 @14:07:03.86 SPE.coast @14:07:03.92 SPE.configure SPEconfig @14:07:04.01 AS.reconfigure ASconfig @14:07:04.06 AS.hold @14:07:04.12 AS.configure ASconfig @14:07:04.22 SS.reconfigure SSconfig @14:07:04.27 SS.hold @14:07:04.32 SS.configure SSconfig @14:07:04.42 CC.reconfigure CCconfig @14:07:04.46 CC.hold @14:07:04.53 CC.configure CCconfig @14:07:04.63 CS.reconfigure CSconfig @14:07:04.67 CS.hold @14:07:04.75 CS.configure CSconfig @14:07:05.23 PC.reconfigure PCconfig @14:07:05.27 PC.hold @14:07:05.33 PC.configure PCconfig @14:07:05.42 PS.configure PSconfig @14:07:05.54 Forearm.reconfigure LooseForearm @14:07:05.58 Forearm.hold @14:07:05.64 Forearm.configure LooseForearm @14:07:05.74 Elbow.reconfigure LooseElbow @14:07:05.78 Elbow.hold @14:07:05.85 Elbow.configure LooseElbow @14:07:05.97 SC.reconfigure SCloose @14:07:06.01 SC.hold @14:07:06.06 SC.configure SCloose @14:07:06.15 SE.reconfigure SEconfig @14:07:06.20 SE.hold @14:07:06.26 SE.configure SEconfig @14:07:06.31 CH.coast @14:07:06.36 CH.configure CHconfig @14:07:06.39 PH.coast @14:07:06.45 PH.configure PHconfig @14:07:06.49 PCR.off @14:07:06.52 PCR.pidtemp= 50,0.1,0,300,25,25 @14:07:06.55 PCR.caltemp= 2.8814,0.0372,0 @14:07:06.58 All dwarf controllers running firmware version 3.75 @14:07:06.73 Cleanly exited at 14:04:07CEST29-Jul-20 @14:07:06.76 Loading /home/esp/esp2/mission/stdMER2020MWJ.rb mission @14:07:07.38 AFV.select :carrierSolution @14:07:07.49 delay 2 seconds @14:07:09.52 AS.seek 0.5ml @14:07:35.22 Session Begun @14:07:38.61
delay 1 second @14:07:39.63 AFV.select :holdingCoil @14:07:39.74 ATV.dial :extSrc @14:07:41.10 AS.reconfigure ASslow1 @14:07:41.17 AS.seek 0.53ml @14:07:47.47 delay 5 seconds @14:07:52.57 AS.reconfigure ASslow2 @14:07:52.64 AS.seek 0.54ml @14:08:03.16 delay 20 seconds @14:08:23.24 AS.reconfigure ASslow1 @14:08:23.31 AS.seek 0.57ml @14:08:29.64 delay 5 seconds @14:08:34.74 AS.reconfigure ASslow2 @14:08:34.80 AS.seek 0.58ml @14:08:45.05 delay 20 seconds @14:09:05.13 AS.reconfigure ASslow1 @14:09:05.20 AS.seek 0.61ml @14:09:11.50 delay 5 seconds @14:09:16.58 AS.reconfigure ASslow2 @14:09:16.65 AS.seek 0.62ml @14:09:26.85 delay 20 seconds @14:09:46.93 AS.reconfigure ASslow1 @14:09:47.00 AS.seek 0.65ml @14:09:53.30 delay 5 seconds @14:09:58.38 AS.reconfigure ASslow2 @14:09:58.45 AS.seek 0.66ml @14:10:08.75 delay 20 seconds @14:10:24.68 -> exit @14:10:24.69 Session Ended @14:10:28.77
AS.reconfigure ASslow1 @14:10:28.83 AS.seek 0.705ml @14:10:38.14 delay 10 seconds @14:10:48.17 ATV.dial :air1 @14:10:49.18 Session Begun @14:10:49.92
AS.seek 0.725ml @14:10:54.22 delay 3 seconds @14:10:57.24 ATV.dial :waste @14:10:57.80 AS.reconfigure ASslow0 @14:10:57.87 AS.seek :empty @14:11:04.27 -> CG @14:12:11.38 Setting up PCR for CG -- writing /var/log/esp/CG20jul2914h.pcr @14:12:11.41 Building PCR Slug from extSrc with MM1 mix, PP2 primer @14:12:11.44 Priming with carrier @14:12:11.46 ATV.dial ARV @14:12:12.97 ARV.dial :carrier @14:12:13.35 AS.reconfigure ASnorm @14:12:13.43 AS.seek 0.499ml @14:12:38.96 AFV.select :carrierSolution @14:12:39.10 delay 2 seconds @14:12:41.13 AS.reconfigure ASconfig @14:12:41.20 AS.seek 1ml @14:13:10.27 delay 1 second @14:13:11.29 AFV.select :holdingCoil @14:13:11.41 delay 1 second @14:13:12.44 ATV.dial :waste @14:13:14.13 AS.reconfigure ASnorm @14:13:14.20 AS.seek :empty @14:14:04.96 AFV.select :carrierSolution @14:14:05.10 delay 2 seconds @14:14:07.13 AS.reconfigure ASconfig @14:14:07.20 AS.seek 0.5ml @14:14:36.30 delay 1 second @14:14:37.32 AFV.select :holdingCoil @14:14:37.44 Building Slug @14:14:37.46 ATV.dial :ARV @14:14:39.13 delay 2 seconds @14:14:41.16 ARV.dial :air1 @14:14:41.58 delay 2 seconds @14:14:43.66 AS.reconfigure ASslow2 @14:14:43.74 AS.seek 0.506ml @14:14:49.96 delay 2 seconds @14:14:51.99 ARV.dial :MM1 @14:14:52.61 delay 2 seconds @14:14:54.64 Adding MM1 @14:14:54.73 AS.seek 0.524ml @14:15:13.14 delay 25 seconds @14:15:38.17 ARV.dial :PP2 @14:15:38.58 Adding PP2 @14:15:38.59 delay 2 seconds @14:15:40.68 AS.seek 0.53ml @14:15:47.14 delay 10 seconds @14:15:57.17 ARV.dial :air2 @14:15:57.57 adding ARV air @14:15:57.58 delay 2 seconds @14:15:59.68 AS.seek 0.536ml @14:16:06.28 delay 5 seconds @14:16:11.31 ARV.dial :carrier @14:16:12.63 delay 7 seconds @14:16:19.72 AS.seek 0.594ml @14:17:18.38 delay 5 seconds @14:17:23.41 ATV.dial :extSrc @14:17:23.99 delay 2 seconds @14:17:26.10 AS.seek 0.6ml @14:17:32.39 delay 4 seconds @14:17:36.42 ATV.dial ARV @14:17:36.99 delay 2 seconds @14:17:39.02 ARV.dial :carrier @14:17:39.04 delay 2 seconds @14:17:41.13 AS.seek 0.65ml @14:18:31.80 delay 4 seconds @14:18:35.83 ATV.dial PCR @14:18:36.61 ASV.dial :waste1 @14:18:37.17 ARV.dial :air1 @14:18:37.58 Positioning Slug by 0.324ml @14:18:37.69 AS.seek 0.326ml @14:24:03.74 delay 5 seconds @14:24:08.77 ATV.dial ARV @14:24:09.57 ASV.dial :PCRlock @14:24:09.97 delay 5 seconds @14:24:15.00 Running CG Profile60C300 thermocycling @14:24:15.02 PCR.program Profile60C300 @14:24:15.14 Profile60C300 should complete in < 1:59:25 @14:24:15.16 PCR.run 2:34:16.25 @16:04:08.08 PCR.off @16:04:08.75 PCR for CG completed -- closed /var/log/esp/CG20jul2914h.pcr @16:04:08.84 Cleaning PCR @16:04:08.85 AFV.select :carrierSolution @16:04:08.99 delay 2 seconds @16:04:11.02 AS.reconfigure ASconfig @16:04:11.09 AS.seek 0.5ml @16:04:21.40 delay 1 second @16:04:22.41 AFV.select :holdingCoil @16:04:22.53 ASV.dial :waste1 @16:04:22.93 ATV.dial :air3 @16:04:24.26 AS.reconfigure ASslow1 @16:04:24.33 AS.seek 0.51ml @16:04:26.63 delay 2 seconds @16:04:28.65 ATV.dial :bleach @16:04:29.30 AS.seek 0.81ml @16:05:29.89 delay 3 seconds @16:05:32.91 ATV.dial :air3 @16:05:33.50 delay 3 seconds @16:05:36.58 AS.seek 0.835ml @16:05:41.87 delay 3 seconds @16:05:44.90 ATV.dial :bleach @16:05:45.53 AS.seek 0.82ml @16:05:48.85 delay 3 seconds @16:05:51.87 ARV.dial :waste @16:05:52.50 delay 3 seconds @16:05:55.53 ATV.dial :ARV @16:05:56.45 AS.reconfigure ASconfig @16:05:56.52 AS.seek 0.47ml @16:06:16.95 AFV.select :carrierSolution @16:06:17.08 delay 2 seconds @16:06:19.11 AS.seek 1ml @16:06:49.92 delay 1 second @16:06:50.93 AFV.select :holdingCoil @16:06:51.06 delay 1 second @16:06:52.09 AS.reconfigure ASfast @16:06:52.16 AS.seek 0ml @16:07:19.52 AFV.select :carrierSolution @16:07:19.66 delay 2 seconds @16:07:21.69 AS.reconfigure ASconfig @16:07:21.76 AS.seek 1ml @16:08:19.57 delay 1 second @16:08:20.58 AFV.select :holdingCoil @16:08:20.71 delay 1 second @16:08:21.74 AS.reconfigure ASfast @16:08:21.81 AS.seek 0ml @16:08:49.17 AFV.select :carrierSolution @16:08:49.31 delay 2 seconds @16:08:51.34 AS.reconfigure ASconfig @16:08:51.41 AS.seek 1ml @16:09:49.21 delay 1 second @16:09:50.23 AFV.select :holdingCoil @16:09:50.35 delay 1 second @16:09:51.38 AS.reconfigure ASfast @16:09:51.45 AS.seek 0ml @16:10:18.82 ARV.dial between carrier and air1 @16:10:19.34 ATV.dial :air3 @16:10:20.66 AS.reconfigure ASslow1 @16:10:20.73 AS.seek 0.01ml @16:10:23.10 delay 2 seconds @16:10:25.12 ATV.dial :bleach @16:10:25.77 AS.seek 0.81ml @16:13:06.92 delay 3 seconds @16:13:09.95 ATV.dial :air3 @16:13:10.54 delay 3 seconds @16:13:13.63 AS.seek 0.835ml @16:13:18.98 delay 3 seconds @16:13:22.00 ATV.dial :bleach @16:13:22.66 AS.seek 0.82ml @16:13:25.95 delay 3 seconds @16:13:28.98 ATV.dial PCR @16:13:29.39 AS.reconfigure ASconfig @16:13:29.46 AS.seek :empty @16:14:16.92 PCR.program :bleachProfile @16:14:16.99 PCR program should complete in < 2:45 @16:14:17.01 PCR.run 10:00 @16:17:09.75 PCR.off @16:17:09.79 AFV.select :carrierSolution @16:17:09.92 delay 2 seconds @16:17:11.95 AS.seek 1ml @16:18:09.76 delay 1 second @16:18:10.77 AFV.select :holdingCoil @16:18:10.89 delay 1 second @16:18:11.92 AS.reconfigure ASfast @16:18:12.46 AS.seek 0ml @16:18:39.85 delay 3 seconds @16:18:42.88 AFV.select :carrierSolution @16:18:43.00 delay 2 seconds @16:18:45.03 AS.reconfigure ASconfig @16:18:45.11 AS.seek 1ml @16:19:42.95 delay 1 second @16:19:43.96 AFV.select :holdingCoil @16:19:44.09 delay 1 second @16:19:45.12 AS.reconfigure ASfast @16:19:45.19 AS.seek 0ml @16:20:12.59 delay 3 seconds @16:20:15.62 AFV.select :carrierSolution @16:20:15.74 delay 2 seconds @16:20:17.77 AS.reconfigure ASconfig @16:20:17.84 AS.seek 1ml @16:21:15.65 delay 1 second @16:21:16.66 AFV.select :holdingCoil @16:21:16.78 delay 1 second @16:21:17.81 AS.reconfigure ASfast @16:21:17.88 AS.seek 0ml @16:21:45.26 delay 3 seconds @16:21:48.29 AFV.select :carrierSolution @16:21:48.41 delay 2 seconds @16:21:50.44 AS.reconfigure ASconfig @16:21:50.52 AS.seek 1ml @16:22:48.32 delay 1 second @16:22:49.34 AFV.select :holdingCoil @16:22:49.46 delay 1 second @16:22:50.49 AS.reconfigure ASfast @16:22:50.56 AS.seek 0ml @16:23:17.93 delay 3 seconds @16:23:20.95 AFV.select :carrierSolution @16:23:21.07 delay 2 seconds @16:23:23.10 AS.reconfigure ASconfig @16:23:23.18 AS.seek 1ml @16:24:20.99 delay 1 second @16:24:22.00 AFV.select :holdingCoil @16:24:22.12 delay 1 second @16:24:23.15 AS.reconfigure ASnorm @16:24:23.22 AS.seek 0ml @16:25:13.95
AFV.select :carrierSolution @16:25:14.07 delay 2 seconds @16:25:16.09 AS.reconfigure ASconfig @16:25:16.16 AS.seek 0.5ml @16:25:45.23 delay 1 second @16:25:46.24 AFV.select :holdingCoil @16:25:46.36 ATV.dial :air1 @16:25:47.90 AS.reconfigure ASslow2 @16:25:47.97 AS.seek 0.506ml @16:25:54.24 ATV.dial :extSrc @16:25:55.97 AS.seek 0.512ml @16:26:02.38 delay 5 seconds @16:26:07.41 ATV.dial :air1 @16:26:09.18 AS.reconfigure ASslow1 @16:26:09.25 AS.seek 0.542ml @16:26:15.54 ATV.dial :waste @16:26:16.10 AS.reconfigure ASconfig @16:26:16.16 AS.seek :empty @16:26:47.86 Setting up PCR for CG -- writing /var/log/esp/CG20jul2916h.pcr @16:26:47.89 Building PCR Slug from extSrc with MM1 mix, PP2 primer @16:26:47.92 Priming with carrier @16:26:47.94 ATV.dial ARV @16:26:49.46 ARV.dial :carrier @16:26:49.83 AS.reconfigure ASnorm @16:26:49.90 AS.seek 0.499ml @16:27:15.55 AFV.select :carrierSolution @16:27:15.69 delay 2 seconds @16:27:17.72 AS.reconfigure ASconfig @16:27:17.79 AS.seek 1ml @16:27:46.92 delay 1 second @16:27:47.94 AFV.select :holdingCoil @16:27:48.06 delay 1 second @16:27:49.09 ATV.dial :waste @16:27:50.81 AS.reconfigure ASnorm @16:27:50.89 AS.seek :empty @16:28:41.64 AFV.select :carrierSolution @16:28:41.78 delay 2 seconds @16:28:43.81 AS.reconfigure ASconfig @16:28:43.88 AS.seek 0.5ml @16:29:12.99 delay 1 second @16:29:14.00 AFV.select :holdingCoil @16:29:14.13 Building Slug @16:29:14.14 ATV.dial :ARV @16:29:15.84 delay 2 seconds @16:29:17.87 ARV.dial :air1 @16:29:18.28 delay 2 seconds @16:29:20.36 AS.reconfigure ASslow2 @16:29:20.44 AS.seek 0.506ml @16:29:26.68 delay 2 seconds @16:29:28.70 ARV.dial :MM1 @16:29:29.32 delay 2 seconds @16:29:31.35 Adding MM1 @16:29:31.44 AS.seek 0.524ml @16:29:49.96 delay 25 seconds @16:30:14.99 ARV.dial :PP2 @16:30:15.40 Adding PP2 @16:30:15.42 delay 2 seconds @16:30:17.51 AS.seek 0.53ml @16:30:23.73 delay 10 seconds @16:30:33.76 ARV.dial :air2 @16:30:34.15 adding ARV air @16:30:34.17 delay 2 seconds @16:30:36.26 AS.seek 0.536ml @16:30:42.52 delay 5 seconds @16:30:47.55 ARV.dial :carrier @16:30:48.85 delay 7 seconds @16:30:55.93 AS.seek 0.594ml @16:31:54.51 delay 5 seconds @16:31:59.54 ATV.dial :extSrc @16:32:00.12 delay 2 seconds @16:32:02.20 AS.seek 0.6ml @16:32:08.63 delay 4 seconds @16:32:12.66 ATV.dial ARV @16:32:13.23 delay 2 seconds @16:32:15.26 ARV.dial :carrier @16:32:15.27 delay 2 seconds @16:32:17.37 AS.seek 0.65ml @16:33:07.85 delay 4 seconds @16:33:11.88 ATV.dial PCR @16:33:12.66 ASV.dial :waste1 @16:33:12.67 ARV.dial :air1 @16:33:13.11 Positioning Slug by 0.324ml @16:33:13.22 AS.seek 0.326ml @16:38:39.62 delay 5 seconds @16:38:44.65 ATV.dial ARV @16:38:45.45 ASV.dial :PCRlock @16:38:45.85 delay 5 seconds @16:38:50.88 Running CG Profile60C300 thermocycling @16:38:50.89 PCR.program Profile60C300 @16:38:51.01 Profile60C300 should complete in < 1:59:25 @16:38:51.03 PCR.run 2:34:16.25 @18:18:48.40 PCR.off @18:18:49.09 PCR for CG completed -- closed /var/log/esp/CG20jul2916h.pcr @18:18:49.17 Cleaning PCR @18:18:49.18 AFV.select :carrierSolution @18:18:49.32 delay 2 seconds @18:18:51.35 AS.reconfigure ASconfig @18:18:51.42 AS.seek 0.5ml @18:19:01.74 delay 1 second @18:19:02.75 AFV.select :holdingCoil @18:19:02.88 ASV.dial :waste1 @18:19:03.28 ATV.dial :air3 @18:19:04.59 AS.reconfigure ASslow1 @18:19:04.66 AS.seek 0.51ml @18:19:06.96 delay 2 seconds @18:19:08.98 ATV.dial :bleach @18:19:09.63 AS.seek 0.81ml @18:20:10.23 delay 3 seconds @18:20:13.25 ATV.dial :air3 @18:20:13.83 delay 3 seconds @18:20:16.91 AS.seek 0.835ml @18:20:22.21 delay 3 seconds @18:20:25.23 ATV.dial :bleach @18:20:25.88 AS.seek 0.82ml @18:20:29.20 delay 3 seconds @18:20:32.22 ARV.dial :waste @18:20:32.84 delay 3 seconds @18:20:35.87 ATV.dial :ARV @18:20:36.78 AS.reconfigure ASconfig @18:20:36.85 AS.seek 0.47ml @18:20:57.29 AFV.select :carrierSolution @18:20:57.42 delay 2 seconds @18:20:59.45 AS.seek 1ml @18:21:30.22 delay 1 second @18:21:31.24 AFV.select :holdingCoil @18:21:31.36 delay 1 second @18:21:32.39 AS.reconfigure ASfast @18:21:32.45 AS.seek 0ml @18:21:59.80 AFV.select :carrierSolution @18:21:59.94 delay 2 seconds @18:22:01.97 AS.reconfigure ASconfig @18:22:02.04 AS.seek 1ml @18:22:59.84 delay 1 second @18:23:00.85 AFV.select :holdingCoil @18:23:00.97 delay 1 second @18:23:02.00 AS.reconfigure ASfast @18:23:02.06 AS.seek 0ml @18:23:29.43 AFV.select :carrierSolution @18:23:29.57 delay 2 seconds @18:23:31.60 AS.reconfigure ASconfig @18:23:31.67 AS.seek 1ml @18:24:29.48 delay 1 second @18:24:30.49 AFV.select :holdingCoil @18:24:30.61 delay 1 second @18:24:31.64 AS.reconfigure ASfast @18:24:31.71 AS.seek 0ml @18:24:59.07 ARV.dial between carrier and air1 @18:24:59.61 ATV.dial :air3 @18:25:00.94 AS.reconfigure ASslow1 @18:25:01.01 AS.seek 0.01ml @18:25:03.33 delay 2 seconds @18:25:05.35 ATV.dial :bleach @18:25:06.01 AS.seek 0.81ml @18:27:47.17 delay 3 seconds @18:27:50.19 ATV.dial :air3 @18:27:50.77 delay 3 seconds @18:27:53.85 AS.seek 0.835ml @18:27:59.17 delay 3 seconds @18:28:02.20 ATV.dial :bleach @18:28:02.84 AS.seek 0.82ml @18:28:06.16 delay 3 seconds @18:28:09.18 ATV.dial PCR @18:28:09.59 AS.reconfigure ASconfig @18:28:09.66 AS.seek :empty @18:28:57.14 PCR.program :bleachProfile @18:28:57.21 PCR program should complete in < 2:45 @18:28:57.22 PCR.run 10:00 @18:31:49.98 PCR.off @18:31:50.02 AFV.select :carrierSolution @18:31:50.14 delay 2 seconds @18:31:52.17 AS.seek 1ml @18:32:49.97 delay 1 second @18:32:50.98 AFV.select :holdingCoil @18:32:51.10 delay 1 second @18:32:52.13 AS.reconfigure ASfast @18:32:52.20 AS.seek 0ml @18:33:19.58 delay 3 seconds @18:33:22.59 AFV.select :carrierSolution @18:33:22.72 delay 2 seconds @18:33:24.75 AS.reconfigure ASconfig @18:33:24.82 AS.seek 1ml @18:34:22.61 delay 1 second @18:34:23.62 AFV.select :holdingCoil @18:34:23.74 delay 1 second @18:34:24.77 AS.reconfigure ASfast @18:34:24.84 AS.seek 0ml @18:34:52.20 delay 3 seconds @18:34:55.23 AFV.select :carrierSolution @18:34:55.35 delay 2 seconds @18:34:57.38 AS.reconfigure ASconfig @18:34:57.46 AS.seek 1ml @18:35:55.23 delay 1 second @18:35:56.25 AFV.select :holdingCoil @18:35:56.37 delay 1 second @18:35:57.40 AS.reconfigure ASfast @18:35:57.47 AS.seek 0ml @18:36:24.84 delay 3 seconds @18:36:27.86 AFV.select :carrierSolution @18:36:27.98 delay 2 seconds @18:36:30.01 AS.reconfigure ASconfig @18:36:30.09 AS.seek 1ml @18:37:27.91 delay 1 second @18:37:28.92 AFV.select :holdingCoil @18:37:29.04 delay 1 second @18:37:30.07 AS.reconfigure ASfast @18:37:30.14 AS.seek 0ml @18:37:57.50 delay 3 seconds @18:38:00.52 AFV.select :carrierSolution @18:38:00.64 delay 2 seconds @18:38:02.67 AS.reconfigure ASconfig @18:38:02.75 AS.seek 1ml @18:39:01.03 delay 1 second @18:39:02.04 AFV.select :holdingCoil @18:39:02.16 delay 1 second @18:39:03.19 AS.reconfigure ASnorm @18:39:03.26 AS.seek 0ml @18:39:53.99
AFV.select :carrierSolution @18:39:54.12 delay 2 seconds @18:39:56.14 AS.reconfigure ASconfig @18:39:56.21 AS.seek 0.5ml @18:40:25.28 delay 1 second @18:40:26.29 AFV.select :holdingCoil @18:40:26.42 ATV.dial :air1 @18:40:27.96 AS.reconfigure ASslow2 @18:40:28.02 AS.seek 0.51ml @18:40:38.37 delay 5 seconds @18:40:43.40 ATV.dial :extSrc @18:40:45.15 AS.seek 0.53ml @18:41:05.55 delay 10 seconds @18:41:15.58 ATV.dial :air1 @18:41:17.32 AS.reconfigure ASslow1 @18:41:17.39 AS.seek 0.56ml @18:41:23.72 ATV.dial :waste @18:41:24.30 AS.reconfigure ASconfig @18:41:24.36 AS.seek :empty @18:41:57.08 Setting up PCR for CG -- writing /var/log/esp/CG20jul2918h.pcr @18:41:57.11 Building PCR Slug from extSrc with MM1 mix, PP2 primer @18:41:57.14 Priming with carrier @18:41:57.16 ATV.dial ARV @18:41:58.67 ARV.dial :carrier @18:41:59.07 AS.reconfigure ASnorm @18:41:59.14 AS.seek 0.499ml @18:42:24.72 AFV.select :carrierSolution @18:42:24.86 delay 2 seconds @18:42:26.89 AS.reconfigure ASconfig @18:42:26.96 AS.seek 1ml @18:42:56.05 delay 1 second @18:42:57.07 AFV.select :holdingCoil @18:42:57.19 delay 1 second @18:42:58.22 ATV.dial :waste @18:42:59.92 AS.reconfigure ASnorm @18:42:59.99 AS.seek :empty @18:43:50.75 AFV.select :carrierSolution @18:43:50.88 delay 2 seconds @18:43:52.91 AS.reconfigure ASconfig @18:43:52.98 AS.seek 0.5ml @18:44:22.11 delay 1 second @18:44:23.12 AFV.select :holdingCoil @18:44:23.25 Building Slug @18:44:23.26 ATV.dial :ARV @18:44:24.92 delay 2 seconds @18:44:26.95 ARV.dial :air1 @18:44:27.37 delay 2 seconds @18:44:29.45 AS.reconfigure ASslow2 @18:44:29.53 AS.seek 0.506ml @18:44:36.11 delay 2 seconds @18:44:38.13 ARV.dial :MM1 @18:44:38.75 delay 2 seconds @18:44:40.78 Adding MM1 @18:44:40.87 AS.seek 0.524ml @18:44:59.26 delay 25 seconds @18:45:24.29 ARV.dial :PP2 @18:45:24.70 Adding PP2 @18:45:24.72 delay 2 seconds @18:45:26.81 AS.seek 0.53ml @18:45:33.01 delay 10 seconds @18:45:43.04 ARV.dial :air2 @18:45:43.44 adding ARV air @18:45:43.46 delay 2 seconds @18:45:45.55 AS.seek 0.536ml @18:45:51.82 delay 5 seconds @18:45:56.86 ARV.dial :carrier @18:45:58.16 delay 7 seconds @18:46:05.25 AS.seek 0.594ml @18:47:03.75 delay 5 seconds @18:47:08.78 ATV.dial :extSrc @18:47:09.34 delay 2 seconds @18:47:11.43 AS.seek 0.6ml @18:47:17.86 delay 4 seconds @18:47:21.89 ATV.dial ARV @18:47:22.45 delay 2 seconds @18:47:24.48 ARV.dial :carrier @18:47:24.49 delay 2 seconds @18:47:26.59 AS.seek 0.65ml @18:48:17.08 delay 4 seconds @18:48:21.11 ATV.dial PCR @18:48:21.89 ASV.dial :waste1 @18:48:21.90 ARV.dial :air1 @18:48:22.34 Positioning Slug by 0.324ml @18:48:22.45 AS.seek 0.326ml @18:53:48.76 delay 5 seconds @18:53:53.79 ATV.dial ARV @18:53:54.59 ASV.dial :PCRlock @18:53:55.00 delay 5 seconds @18:54:00.03 Running CG Profile60C300 thermocycling @18:54:00.04 PCR.program Profile60C300 @18:54:00.16 Profile60C300 should complete in < 1:59:25 @18:54:00.55 PCR.run 2:34:16.25 @20:33:10.82 PCR.off @20:33:11.51 PCR for CG completed -- closed /var/log/esp/CG20jul2918h.pcr @20:33:11.60 Cleaning PCR @20:33:11.61 AFV.select :carrierSolution @20:33:11.74 delay 2 seconds @20:33:13.77 AS.reconfigure ASconfig @20:33:13.84 AS.seek 0.5ml @20:33:24.15 delay 1 second @20:33:25.17 AFV.select :holdingCoil @20:33:25.29 ASV.dial :waste1 @20:33:25.68 ATV.dial :air3 @20:33:27.00 AS.reconfigure ASslow1 @20:33:27.07 AS.seek 0.51ml @20:33:29.38 delay 2 seconds @20:33:31.41 ATV.dial :bleach @20:33:32.06 AS.seek 0.81ml @20:34:32.76 delay 3 seconds @20:34:35.79 ATV.dial :air3 @20:34:36.35 delay 3 seconds @20:34:39.43 AS.seek 0.835ml @20:34:44.75 delay 3 seconds @20:34:47.77 ATV.dial :bleach @20:34:48.41 AS.seek 0.82ml @20:34:51.71 delay 3 seconds @20:34:54.73 ARV.dial :waste @20:34:55.35 delay 3 seconds @20:34:58.38 ATV.dial :ARV @20:34:59.29 AS.reconfigure ASconfig @20:34:59.36 AS.seek 0.47ml @20:35:19.79 AFV.select :carrierSolution @20:35:19.92 delay 2 seconds @20:35:21.95 AS.seek 1ml @20:35:52.74 delay 1 second @20:35:53.75 AFV.select :holdingCoil @20:35:53.88 delay 1 second @20:35:54.91 AS.reconfigure ASfast @20:35:54.98 AS.seek 0ml @20:36:22.34 AFV.select :carrierSolution @20:36:22.48 delay 2 seconds @20:36:24.51 AS.reconfigure ASconfig @20:36:24.58 AS.seek 1ml @20:37:22.38 delay 1 second @20:37:23.39 AFV.select :holdingCoil @20:37:23.52 delay 1 second @20:37:24.55 AS.reconfigure ASfast @20:37:24.62 AS.seek 0ml @20:37:51.98 AFV.select :carrierSolution @20:37:52.12 delay 2 seconds @20:37:54.15 AS.reconfigure ASconfig @20:37:54.22 AS.seek 1ml @20:38:52.02 delay 1 second @20:38:53.03 AFV.select :holdingCoil @20:38:53.16 delay 1 second @20:38:54.19 AS.reconfigure ASfast @20:38:54.26 AS.seek 0ml @20:39:21.64 ARV.dial between carrier and air1 @20:39:22.16 ATV.dial :air3 @20:39:23.47 AS.reconfigure ASslow1 @20:39:23.55 AS.seek 0.01ml @20:39:25.90 delay 2 seconds @20:39:27.92 ATV.dial :bleach @20:39:28.55 AS.seek 0.81ml @20:42:09.73 delay 3 seconds @20:42:12.76 ATV.dial :air3 @20:42:13.33 delay 3 seconds @20:42:16.41 AS.seek 0.835ml @20:42:21.77 delay 3 seconds @20:42:24.79 ATV.dial :bleach @20:42:25.41 AS.seek 0.82ml @20:42:28.74 delay 3 seconds @20:42:31.77 ATV.dial PCR @20:42:32.18 AS.reconfigure ASconfig @20:42:32.25 AS.seek :empty @20:43:19.73 PCR.program :bleachProfile @20:43:19.80 PCR program should complete in < 2:45 @20:43:19.82 PCR.run 10:00 @20:46:13.02 PCR.off @20:46:13.06 AFV.select :carrierSolution @20:46:13.19 delay 2 seconds @20:46:15.22 AS.seek 1ml @20:47:13.05 delay 1 second @20:47:14.06 AFV.select :holdingCoil @20:47:14.19 delay 1 second @20:47:15.22 AS.reconfigure ASfast @20:47:15.29 AS.seek 0ml @20:47:42.66 delay 3 seconds @20:47:45.69 AFV.select :carrierSolution @20:47:45.81 delay 2 seconds @20:47:47.84 AS.reconfigure ASconfig @20:47:47.90 AS.seek 1ml @20:48:45.71 delay 1 second @20:48:46.73 AFV.select :holdingCoil @20:48:46.85 delay 1 second @20:48:47.88 AS.reconfigure ASfast @20:48:47.95 AS.seek 0ml @20:49:15.33 delay 3 seconds @20:49:18.36 AFV.select :carrierSolution @20:49:18.48 delay 2 seconds @20:49:20.51 AS.reconfigure ASconfig @20:49:20.59 AS.seek 1ml @20:50:18.37 delay 1 second @20:50:19.38 AFV.select :holdingCoil @20:50:19.51 delay 1 second @20:50:20.54 AS.reconfigure ASfast @20:50:20.61 AS.seek 0ml @20:50:47.97 delay 3 seconds @20:50:50.99 AFV.select :carrierSolution @20:50:51.12 delay 2 seconds @20:50:53.15 AS.reconfigure ASconfig @20:50:53.23 AS.seek 1ml @20:51:51.01 delay 1 second @20:51:52.02 AFV.select :holdingCoil @20:51:52.15 delay 1 second @20:51:53.18 AS.reconfigure ASfast @20:51:53.25 AS.seek 0ml @20:52:20.72 delay 3 seconds @20:52:23.75 AFV.select :carrierSolution @20:52:23.87 delay 2 seconds @20:52:25.90 AS.reconfigure ASconfig @20:52:25.97 AS.seek 1ml @20:53:23.76 delay 1 second @20:53:24.77 AFV.select :holdingCoil @20:53:24.89 delay 1 second @20:53:25.92 AS.reconfigure ASnorm @20:53:25.99 AS.seek 0ml @20:54:16.73
AFV.select :carrierSolution @20:54:16.86 delay 2 seconds @20:54:18.89 AS.reconfigure ASconfig @20:54:18.96 AS.seek 0.5ml @20:54:48.04 delay 1 second @20:54:49.05 AFV.select :holdingCoil @20:54:49.17 ATV.dial :air1 @20:54:50.69 AS.reconfigure ASslow2 @20:54:50.76 AS.seek 0.506ml @20:54:56.96 ATV.dial :extSrc @20:54:58.69 AS.seek 0.512ml @20:55:05.10 delay 5 seconds @20:55:10.13 ATV.dial :air1 @20:55:11.88 AS.reconfigure ASslow1 @20:55:11.94 AS.seek 0.542ml @20:55:18.27 ATV.dial :waste @20:55:18.83 AS.reconfigure ASconfig @20:55:18.89 AS.seek :empty @20:55:50.58 Setting up PCR for CG -- writing /var/log/esp/CG20jul2920h.pcr @20:55:50.61 Building PCR Slug from extSrc with MM1 mix, PP2 primer @20:55:50.64 Priming with carrier @20:55:50.66 ATV.dial ARV @20:55:52.27 ARV.dial :carrier @20:55:52.63 AS.reconfigure ASnorm @20:55:52.71 AS.seek 0.499ml @20:56:18.29 AFV.select :carrierSolution @20:56:18.43 delay 2 seconds @20:56:20.46 AS.reconfigure ASconfig @20:56:20.53 AS.seek 1ml @20:56:49.61 delay 1 second @20:56:50.63 AFV.select :holdingCoil @20:56:50.75 delay 1 second @20:56:51.78 ATV.dial :waste @20:56:53.47 AS.reconfigure ASnorm @20:56:53.55 AS.seek :empty @20:57:44.31 AFV.select :carrierSolution @20:57:44.45 delay 2 seconds @20:57:46.48 AS.reconfigure ASconfig @20:57:46.55 AS.seek 0.5ml @20:58:15.71 delay 1 second @20:58:16.73 AFV.select :holdingCoil @20:58:16.85 Building Slug @20:58:16.86 ATV.dial :ARV @20:58:18.54 delay 2 seconds @20:58:20.57 ARV.dial :air1 @20:58:20.97 delay 2 seconds @20:58:23.05 AS.reconfigure ASslow2 @20:58:23.13 AS.seek 0.506ml @20:58:29.33 delay 2 seconds @20:58:31.35 ARV.dial :MM1 @20:58:31.96 delay 2 seconds @20:58:33.99 Adding MM1 @20:58:34.08 AS.seek 0.524ml @20:58:52.33 delay 25 seconds @20:59:17.36 ARV.dial :PP2 @20:59:17.77 Adding PP2 @20:59:17.79 delay 2 seconds @20:59:19.87 AS.seek 0.53ml @20:59:26.08 delay 10 seconds @20:59:36.11 ARV.dial :air2 @20:59:36.92 adding ARV air @20:59:36.96 delay 2 seconds @20:59:39.06 AS.seek 0.536ml @20:59:45.51 delay 5 seconds @20:59:50.54 ARV.dial :carrier @20:59:51.85 delay 7 seconds @20:59:58.94 AS.seek 0.594ml @21:00:57.60 delay 5 seconds @21:01:02.63 ATV.dial :extSrc @21:01:03.21 delay 2 seconds @21:01:05.30 AS.seek 0.6ml @21:01:11.77 delay 4 seconds @21:01:15.80 ATV.dial ARV @21:01:16.36 delay 2 seconds @21:01:18.39 ARV.dial :carrier @21:01:18.40 delay 2 seconds @21:01:20.50 AS.seek 0.65ml @21:02:10.96 delay 4 seconds @21:02:14.99 ATV.dial PCR @21:02:15.79 ASV.dial :waste1 @21:02:15.83 ARV.dial :air1 @21:02:16.30 Positioning Slug by 0.324ml @21:02:16.41 AS.seek 0.326ml @21:07:42.47 delay 5 seconds @21:07:47.50 ATV.dial ARV @21:07:48.30 ASV.dial :PCRlock @21:07:48.71 delay 5 seconds @21:07:53.74 Running CG Profile60C300 thermocycling @21:07:53.75 PCR.program Profile60C300 @21:07:53.88 Profile60C300 should complete in < 1:59:25 @21:07:53.90 PCR.run 2:34:16.25 @22:47:16.68 PCR.off @22:47:17.36 PCR for CG completed -- closed /var/log/esp/CG20jul2920h.pcr @22:47:17.45 Cleaning PCR @22:47:17.46 AFV.select :carrierSolution @22:47:17.58 delay 2 seconds @22:47:19.61 AS.reconfigure ASconfig @22:47:19.68 AS.seek 0.5ml @22:47:30.00 delay 1 second @22:47:31.01 AFV.select :holdingCoil @22:47:31.14 ASV.dial :waste1 @22:47:31.53 ATV.dial :air3 @22:47:32.86 AS.reconfigure ASslow1 @22:47:32.94 AS.seek 0.51ml @22:47:35.21 delay 2 seconds @22:47:37.23 ATV.dial :bleach @22:47:37.88 AS.seek 0.81ml @22:48:38.48 delay 3 seconds @22:48:41.51 ATV.dial :air3 @22:48:42.08 delay 3 seconds @22:48:45.16 AS.seek 0.835ml @22:48:50.46 delay 3 seconds @22:48:53.48 ATV.dial :bleach @22:48:54.11 AS.seek 0.82ml @22:48:57.44 delay 3 seconds @22:49:00.46 ARV.dial :waste @22:49:01.09 delay 3 seconds @22:49:04.12 ATV.dial :ARV @22:49:05.03 AS.reconfigure ASconfig @22:49:05.10 AS.seek 0.47ml @22:49:25.50 AFV.select :carrierSolution @22:49:25.63 delay 2 seconds @22:49:27.66 AS.seek 1ml @22:49:58.47 delay 1 second @22:49:59.49 AFV.select :holdingCoil @22:49:59.61 delay 1 second @22:50:00.64 AS.reconfigure ASfast @22:50:00.71 AS.seek 0ml @22:50:28.05 AFV.select :carrierSolution @22:50:28.19 delay 2 seconds @22:50:30.22 AS.reconfigure ASconfig @22:50:30.29 AS.seek 1ml @22:51:28.04 delay 1 second @22:51:29.05 AFV.select :holdingCoil @22:51:29.18 delay 1 second @22:51:30.21 AS.reconfigure ASfast @22:51:30.28 AS.seek 0ml @22:51:57.64 AFV.select :carrierSolution @22:51:57.78 delay 2 seconds @22:51:59.81 AS.reconfigure ASconfig @22:51:59.88 AS.seek 1ml @22:52:57.69 delay 1 second @22:52:58.70 AFV.select :holdingCoil @22:52:58.83 delay 1 second @22:53:00.25 AS.reconfigure ASfast @22:53:00.39 AS.seek 0ml @22:53:27.76 ARV.dial between carrier and air1 @22:53:28.28 ATV.dial :air3 @22:53:29.60 AS.reconfigure ASslow1 @22:53:29.68 AS.seek 0.01ml @22:53:32.04 delay 2 seconds @22:53:34.06 ATV.dial :bleach @22:53:34.71 AS.seek 0.81ml @22:56:15.86 delay 3 seconds @22:56:18.89 ATV.dial :air3 @22:56:19.45 delay 3 seconds @22:56:22.54 AS.seek 0.835ml @22:56:27.83 delay 3 seconds @22:56:30.85 ATV.dial :bleach @22:56:31.49 AS.seek 0.82ml @22:56:34.83 delay 3 seconds @22:56:37.86 ATV.dial PCR @22:56:38.29 AS.reconfigure ASconfig @22:56:38.36 AS.seek :empty @22:57:25.83 PCR.program :bleachProfile @22:57:25.90 PCR program should complete in < 2:45 @22:57:25.91 PCR.run 10:00 @23:00:18.66 PCR.off @23:00:18.70 AFV.select :carrierSolution @23:00:18.82 delay 2 seconds @23:00:20.85 AS.seek 1ml @23:01:18.72 delay 1 second @23:01:19.73 AFV.select :holdingCoil @23:01:19.86 delay 1 second @23:01:20.89 AS.reconfigure ASfast @23:01:20.95 AS.seek 0ml @23:01:48.33 delay 3 seconds @23:01:51.35 AFV.select :carrierSolution @23:01:51.48 delay 2 seconds @23:01:53.51 AS.reconfigure ASconfig @23:01:53.59 AS.seek 1ml @23:02:51.40 delay 1 second @23:02:52.41 AFV.select :holdingCoil @23:02:52.53 delay 1 second @23:02:53.56 AS.reconfigure ASfast @23:02:53.63 AS.seek 0ml @23:03:21.00 delay 3 seconds @23:03:24.02 AFV.select :carrierSolution @23:03:24.15 delay 2 seconds @23:03:26.18 AS.reconfigure ASconfig @23:03:26.26 AS.seek 1ml @23:04:24.09 delay 1 second @23:04:25.10 AFV.select :holdingCoil @23:04:25.23 delay 1 second @23:04:26.26 AS.reconfigure ASfast @23:04:26.33 AS.seek 0ml @23:04:53.70 delay 3 seconds @23:04:56.73 AFV.select :carrierSolution @23:04:56.85 delay 2 seconds @23:04:58.88 AS.reconfigure ASconfig @23:04:58.95 AS.seek 1ml @23:05:56.73 delay 1 second @23:05:57.74 AFV.select :holdingCoil @23:05:57.86 delay 1 second @23:05:58.89 AS.reconfigure ASfast @23:05:58.96 AS.seek 0ml @23:06:26.33 delay 3 seconds @23:06:29.35 AFV.select :carrierSolution @23:06:29.48 delay 2 seconds @23:06:31.51 AS.reconfigure ASconfig @23:06:31.59 AS.seek 1ml @23:07:29.39 delay 1 second @23:07:30.40 AFV.select :holdingCoil @23:07:30.52 delay 1 second @23:07:31.55 AS.reconfigure ASnorm @23:07:31.62 AS.seek 0ml @23:08:22.35
AFV.select :carrierSolution @23:08:22.48 delay 2 seconds @23:08:24.51 AS.reconfigure ASconfig @23:08:24.58 AS.seek 0.5ml @23:08:53.65 delay 1 second @23:08:54.66 AFV.select :holdingCoil @23:08:54.78 ATV.dial :air1 @23:08:56.31 AS.reconfigure ASslow1 @23:08:56.38 AS.seek 0.52ml @23:09:00.70 delay 5 seconds @23:09:05.74 ATV.dial :extSrc @23:09:07.46 AS.reconfigure ASslow2 @23:09:07.53 AS.seek 0.545ml @23:09:32.86 delay 10 seconds @23:09:42.89 ATV.dial :air1 @23:09:44.62 AS.reconfigure ASslow1 @23:09:44.68 AS.seek 0.575ml @23:09:51.01 ATV.dial :waste @23:09:51.58 AS.reconfigure ASconfig @23:09:51.64 AS.seek :empty @23:10:25.20 Setting up PCR for ML -- writing /var/log/esp/ML20jul2923h.pcr @23:10:25.23 Building PCR Slug from extSrc with MM2 mix, PP4 primer @23:10:25.27 Priming with carrier @23:10:25.29 ATV.dial ARV @23:10:26.79 ARV.dial :carrier @23:10:27.14 AS.reconfigure ASnorm @23:10:27.22 AS.seek 0.499ml @23:10:53.22 AFV.select :carrierSolution @23:10:53.36 delay 2 seconds @23:10:55.39 AS.reconfigure ASconfig @23:10:55.46 AS.seek 1ml @23:11:24.56 delay 1 second @23:11:25.59 AFV.select :holdingCoil @23:11:25.71 delay 1 second @23:11:26.74 ATV.dial :waste @23:11:28.43 AS.reconfigure ASnorm @23:11:28.50 AS.seek :empty @23:12:19.24 AFV.select :carrierSolution @23:12:19.38 delay 2 seconds @23:12:21.41 AS.reconfigure ASconfig @23:12:21.48 AS.seek 0.5ml @23:12:50.61 delay 1 second @23:12:51.63 AFV.select :holdingCoil @23:12:51.76 Building Slug @23:12:51.77 ATV.dial :ARV @23:12:53.45 delay 2 seconds @23:12:55.48 ARV.dial :air1 @23:12:55.98 delay 2 seconds @23:12:58.07 AS.reconfigure ASslow2 @23:12:58.14 AS.seek 0.506ml @23:13:04.40 delay 2 seconds @23:13:06.42 ARV.dial :MM2 @23:13:07.93 delay 2 seconds @23:13:09.96 Adding MM2 @23:13:10.05 AS.seek 0.524ml @23:13:28.31 delay 25 seconds @23:13:53.34 ARV.dial :PP4 @23:13:53.75 Adding PP4 @23:13:53.77 delay 2 seconds @23:13:55.87 AS.seek 0.53ml @23:14:02.22 delay 10 seconds @23:14:12.25 ARV.dial :air3 @23:14:12.67 adding ARV air @23:14:12.69 delay 2 seconds @23:14:14.78 AS.seek 0.536ml @23:14:21.25 delay 5 seconds @23:14:26.28 ARV.dial :carrier @23:14:27.57 delay 7 seconds @23:14:34.66 AS.seek 0.594ml @23:15:33.42 delay 5 seconds @23:15:38.45 ATV.dial :extSrc @23:15:39.03 delay 2 seconds @23:15:41.12 AS.seek 0.6ml @23:15:47.42 delay 4 seconds @23:15:51.45 ATV.dial ARV @23:15:52.01 delay 2 seconds @23:15:54.04 ARV.dial :carrier @23:15:54.05 delay 2 seconds @23:15:56.15 AS.seek 0.65ml @23:16:46.92 delay 4 seconds @23:16:50.95 ATV.dial PCR @23:16:51.73 ASV.dial :waste1 @23:16:51.74 ARV.dial :air1 @23:16:52.27 Positioning Slug by 0.324ml @23:16:52.38 AS.seek 0.326ml @23:22:18.59 delay 5 seconds @23:22:23.62 ATV.dial ARV @23:22:24.42 ASV.dial :PCRlock @23:22:24.82 delay 5 seconds @23:22:29.85 Running ML Profile60C350 thermocycling @23:22:29.86 PCR.program Profile60C350 @23:22:30.00 Profile60C350 should complete in < 1:38:55 @23:22:30.01 PCR.run 2:08:38.75 @00:44:09.41CEST30-Jul-20 PCR.off @00:44:10.46 PCR for ML completed -- closed /var/log/esp/ML20jul2923h.pcr @00:44:10.55 Cleaning PCR @00:44:10.56 AFV.select :carrierSolution @00:44:10.69 delay 2 seconds @00:44:12.72 AS.reconfigure ASconfig @00:44:12.79 AS.seek 0.5ml @00:44:23.13 delay 1 second @00:44:24.14 AFV.select :holdingCoil @00:44:24.26 ASV.dial :waste1 @00:44:24.66 ATV.dial :air3 @00:44:25.97 AS.reconfigure ASslow1 @00:44:26.04 AS.seek 0.51ml @00:44:28.33 delay 2 seconds @00:44:30.35 ATV.dial :bleach @00:44:31.00 AS.seek 0.81ml @00:45:31.60 delay 3 seconds @00:45:34.63 ATV.dial :air3 @00:45:35.21 delay 3 seconds @00:45:38.30 AS.seek 0.835ml @00:45:43.61 delay 3 seconds @00:45:46.64 ATV.dial :bleach @00:45:47.30 AS.seek 0.82ml @00:45:50.62 delay 3 seconds @00:45:53.64 ARV.dial :waste @00:45:54.26 delay 3 seconds @00:45:57.29 ATV.dial :ARV @00:45:58.19 AS.reconfigure ASconfig @00:45:58.26 AS.seek 0.47ml @00:46:18.68 AFV.select :carrierSolution @00:46:18.82 delay 2 seconds @00:46:20.85 AS.seek 1ml @00:46:51.66 delay 1 second @00:46:52.68 AFV.select :holdingCoil @00:46:52.80 delay 1 second @00:46:53.83 AS.reconfigure ASfast @00:46:53.90 AS.seek 0ml @00:47:21.26 AFV.select :carrierSolution @00:47:21.40 delay 2 seconds @00:47:23.43 AS.reconfigure ASconfig @00:47:23.50 AS.seek 1ml @00:48:21.30 delay 1 second @00:48:22.32 AFV.select :holdingCoil @00:48:22.44 delay 1 second @00:48:23.47 AS.reconfigure ASfast @00:48:23.54 AS.seek 0ml @00:48:50.92 AFV.select :carrierSolution @00:48:51.05 delay 2 seconds @00:48:53.08 AS.reconfigure ASconfig @00:48:53.15 AS.seek 1ml @00:49:50.91 delay 1 second @00:49:51.92 AFV.select :holdingCoil @00:49:52.05 delay 1 second @00:49:53.08 AS.reconfigure ASfast @00:49:53.15 AS.seek 0ml @00:50:20.53 ARV.dial between carrier and air1 @00:50:21.05 ATV.dial :air3 @00:50:22.38 AS.reconfigure ASslow1 @00:50:22.46 AS.seek 0.01ml @00:50:24.82 delay 2 seconds @00:50:26.84 ATV.dial :bleach @00:50:27.50 AS.seek 0.81ml @00:53:08.66 delay 3 seconds @00:53:11.68 ATV.dial :air3 @00:53:12.24 delay 3 seconds @00:53:15.32 AS.seek 0.835ml @00:53:20.64 delay 3 seconds @00:53:23.67 ATV.dial :bleach @00:53:24.31 AS.seek 0.82ml @00:53:27.65 delay 3 seconds @00:53:30.68 ATV.dial PCR @00:53:31.10 AS.reconfigure ASconfig @00:53:31.16 AS.seek :empty @00:54:18.64 PCR.program :bleachProfile @00:54:18.71 PCR program should complete in < 2:45 @00:54:18.73 PCR.run 10:00 @00:57:11.92 PCR.off @00:57:11.96 AFV.select :carrierSolution @00:57:12.08 delay 2 seconds @00:57:14.11 AS.seek 1ml @00:58:11.97 delay 1 second @00:58:12.98 AFV.select :holdingCoil @00:58:13.11 delay 1 second @00:58:14.14 AS.reconfigure ASfast @00:58:14.21 AS.seek 0ml @00:58:41.58 delay 3 seconds @00:58:44.61 AFV.select :carrierSolution @00:58:44.73 delay 2 seconds @00:58:46.76 AS.reconfigure ASconfig @00:58:46.83 AS.seek 1ml @00:59:44.58 delay 1 second @00:59:45.59 AFV.select :holdingCoil @00:59:45.72 delay 1 second @00:59:46.75 AS.reconfigure ASfast @00:59:46.82 AS.seek 0ml @01:00:14.19 delay 3 seconds @01:00:17.22 AFV.select :carrierSolution @01:00:17.34 delay 2 seconds @01:00:19.37 AS.reconfigure ASconfig @01:00:19.45 AS.seek 1ml @01:01:17.22 delay 1 second @01:01:18.23 AFV.select :holdingCoil @01:01:18.36 delay 1 second @01:01:19.39 AS.reconfigure ASfast @01:01:19.46 AS.seek 0ml @01:01:46.83 delay 3 seconds @01:01:49.86 AFV.select :carrierSolution @01:01:49.98 delay 2 seconds @01:01:52.01 AS.reconfigure ASconfig @01:01:52.09 AS.seek 1ml @01:02:49.94 delay 1 second @01:02:50.95 AFV.select :holdingCoil @01:02:51.08 delay 1 second @01:02:52.11 AS.reconfigure ASfast @01:02:52.18 AS.seek 0ml @01:03:19.57 delay 3 seconds @01:03:22.59 AFV.select :carrierSolution @01:03:22.72 delay 2 seconds @01:03:24.75 AS.reconfigure ASconfig @01:03:24.82 AS.seek 1ml @01:04:22.63 delay 1 second @01:04:23.64 AFV.select :holdingCoil @01:04:23.76 delay 1 second @01:04:24.79 AS.reconfigure ASnorm @01:04:24.86 AS.seek 0ml @01:05:15.59
AFV.select :carrierSolution @01:05:15.72 delay 2 seconds @01:05:17.74 AS.reconfigure ASconfig @01:05:17.81 AS.seek 0.5ml @01:05:46.85 delay 1 second @01:05:47.86 AFV.select :holdingCoil @01:05:47.98 ATV.dial :air1 @01:05:49.52 AS.reconfigure ASslow2 @01:05:49.59 AS.seek 0.506ml @01:05:55.80 ATV.dial :extSrc @01:05:57.53 AS.seek 0.512ml @01:06:03.76 delay 5 seconds @01:06:08.79 ATV.dial :air1 @01:06:10.53 AS.reconfigure ASslow1 @01:06:10.60 AS.seek 0.542ml @01:06:16.88 ATV.dial :waste @01:06:17.44 AS.reconfigure ASconfig @01:06:17.51 AS.seek :empty @01:06:49.20 Setting up PCR for ML -- writing /var/log/esp/ML20jul3001h.pcr @01:06:49.23 Building PCR Slug from extSrc with MM2 mix, PP4 primer @01:06:49.26 Priming with carrier @01:06:49.28 ATV.dial ARV @01:06:50.79 ARV.dial :carrier @01:06:51.14 AS.reconfigure ASnorm @01:06:51.22 AS.seek 0.499ml @01:07:16.85 AFV.select :carrierSolution @01:07:16.99 delay 2 seconds @01:07:19.02 AS.reconfigure ASconfig @01:07:19.09 AS.seek 1ml @01:07:48.16 delay 1 second @01:07:49.18 AFV.select :holdingCoil @01:07:49.30 delay 1 second @01:07:50.33 ATV.dial :waste @01:07:52.02 AS.reconfigure ASnorm @01:07:52.10 AS.seek :empty @01:08:42.85 AFV.select :carrierSolution @01:08:42.99 delay 2 seconds @01:08:45.02 AS.reconfigure ASconfig @01:08:45.09 AS.seek 0.5ml @01:09:14.22 delay 1 second @01:09:15.23 AFV.select :holdingCoil @01:09:15.36 Building Slug @01:09:15.38 ATV.dial :ARV @01:09:17.05 delay 2 seconds @01:09:19.08 ARV.dial :air1 @01:09:19.48 delay 2 seconds @01:09:21.56 AS.reconfigure ASslow2 @01:09:21.63 AS.seek 0.506ml @01:09:27.88 delay 2 seconds @01:09:29.90 ARV.dial :MM2 @01:09:31.41 delay 2 seconds @01:09:33.44 Adding MM2 @01:09:33.54 AS.seek 0.524ml @01:09:51.86 delay 25 seconds @01:10:16.89 ARV.dial :PP4 @01:10:17.30 Adding PP4 @01:10:17.32 delay 2 seconds @01:10:19.42 AS.seek 0.53ml @01:10:25.80 delay 10 seconds @01:10:36.19 ARV.dial :air3 @01:10:36.68 adding ARV air @01:10:36.70 delay 2 seconds @01:10:38.79 AS.seek 0.536ml @01:10:44.99 delay 5 seconds @01:10:50.02 ARV.dial :carrier @01:10:51.32 delay 7 seconds @01:10:58.41 AS.seek 0.594ml @01:11:57.13 delay 5 seconds @01:12:02.16 ATV.dial :extSrc @01:12:02.74 delay 2 seconds @01:12:04.83 AS.seek 0.6ml @01:12:11.15 delay 4 seconds @01:12:15.18 ATV.dial ARV @01:12:15.76 delay 2 seconds @01:12:17.79 ARV.dial :carrier @01:12:17.80 delay 2 seconds @01:12:19.90 AS.seek 0.65ml @01:13:10.35 delay 4 seconds @01:13:14.38 ATV.dial PCR @01:13:15.16 ASV.dial :waste1 @01:13:15.17 ARV.dial :air1 @01:13:15.61 Positioning Slug by 0.324ml @01:13:15.72 AS.seek 0.326ml @01:18:41.77 delay 5 seconds @01:18:46.80 ATV.dial ARV @01:18:47.60 ASV.dial :PCRlock @01:18:48.00 delay 5 seconds @01:18:53.03 Running ML Profile60C350 thermocycling @01:18:53.04 PCR.program Profile60C350 @01:18:53.17 Profile60C350 should complete in < 1:38:55 @01:18:53.18 PCR.run 2:08:38.75 @02:40:14.10 PCR.off @02:40:15.13 PCR for ML completed -- closed /var/log/esp/ML20jul3001h.pcr @02:40:15.22 Cleaning PCR @02:40:15.23 AFV.select :carrierSolution @02:40:15.36 delay 2 seconds @02:40:17.39 AS.reconfigure ASconfig @02:40:17.46 AS.seek 0.5ml @02:40:27.77 delay 1 second @02:40:28.78 AFV.select :holdingCoil @02:40:28.90 ASV.dial :waste1 @02:40:29.30 ATV.dial :air3 @02:40:30.62 AS.reconfigure ASslow1 @02:40:30.69 AS.seek 0.51ml @02:40:32.99 delay 2 seconds @02:40:35.01 ATV.dial :bleach @02:40:35.70 AS.seek 0.81ml @02:41:36.36 delay 3 seconds @02:41:39.38 ATV.dial :air3 @02:41:40.03 delay 3 seconds @02:41:43.12 AS.seek 0.835ml @02:41:48.43 delay 3 seconds @02:41:51.46 ATV.dial :bleach @02:41:52.10 AS.seek 0.82ml @02:41:55.47 delay 3 seconds @02:41:58.50 ARV.dial :waste @02:41:59.12 delay 3 seconds @02:42:02.15 ATV.dial :ARV @02:42:03.06 AS.reconfigure ASconfig @02:42:03.20 AS.seek 0.47ml @02:42:23.63 AFV.select :carrierSolution @02:42:23.77 delay 2 seconds @02:42:25.80 AS.seek 1ml @02:42:56.56 delay 1 second @02:42:57.58 AFV.select :holdingCoil @02:42:57.70 delay 1 second @02:42:58.73 AS.reconfigure ASfast @02:42:58.79 AS.seek 0ml @02:43:26.16 AFV.select :carrierSolution @02:43:26.30 delay 2 seconds @02:43:28.33 AS.reconfigure ASconfig @02:43:28.40 AS.seek 1ml @02:44:26.16 delay 1 second @02:44:27.17 AFV.select :holdingCoil @02:44:27.30 delay 1 second @02:44:28.33 AS.reconfigure ASfast @02:44:28.40 AS.seek 0ml @02:44:55.76 AFV.select :carrierSolution @02:44:55.90 delay 2 seconds @02:44:57.94 AS.reconfigure ASconfig @02:44:58.03 AS.seek 1ml @02:45:55.83 delay 1 second @02:45:56.84 AFV.select :holdingCoil @02:45:56.97 delay 1 second @02:45:58.00 AS.reconfigure ASfast @02:45:58.07 AS.seek 0ml @02:46:25.42 ARV.dial between carrier and air1 @02:46:25.94 ATV.dial :air3 @02:46:27.25 AS.reconfigure ASslow1 @02:46:27.33 AS.seek 0.01ml @02:46:29.73 delay 2 seconds @02:46:31.75 ATV.dial :bleach @02:46:32.38 AS.seek 0.81ml @02:49:13.56 delay 3 seconds @02:49:16.59 ATV.dial :air3 @02:49:17.16 delay 3 seconds @02:49:20.24 AS.seek 0.835ml @02:49:25.54 delay 3 seconds @02:49:28.57 ATV.dial :bleach @02:49:29.20 AS.seek 0.82ml @02:49:32.56 delay 3 seconds @02:49:35.58 ATV.dial PCR @02:49:36.00 AS.reconfigure ASconfig @02:49:36.06 AS.seek :empty @02:50:23.54 PCR.program :bleachProfile @02:50:23.61 PCR program should complete in < 2:45 @02:50:23.62 PCR.run 10:00 @02:53:16.37 PCR.off @02:53:16.41 AFV.select :carrierSolution @02:53:16.53 delay 2 seconds @02:53:18.56 AS.seek 1ml @02:54:16.42 delay 1 second @02:54:17.43 AFV.select :holdingCoil @02:54:17.56 delay 1 second @02:54:18.59 AS.reconfigure ASfast @02:54:18.65 AS.seek 0ml @02:54:46.01 delay 3 seconds @02:54:49.03 AFV.select :carrierSolution @02:54:49.16 delay 2 seconds @02:54:51.19 AS.reconfigure ASconfig @02:54:51.27 AS.seek 1ml @02:55:49.05 delay 1 second @02:55:50.06 AFV.select :holdingCoil @02:55:50.19 delay 1 second @02:55:51.22 AS.reconfigure ASfast @02:55:51.29 AS.seek 0ml @02:56:18.67 delay 3 seconds @02:56:21.70 AFV.select :carrierSolution @02:56:21.82 delay 2 seconds @02:56:23.85 AS.reconfigure ASconfig @02:56:23.92 AS.seek 1ml @02:57:21.73 delay 1 second @02:57:22.74 AFV.select :holdingCoil @02:57:22.87 delay 1 second @02:57:23.90 AS.reconfigure ASfast @02:57:23.97 AS.seek 0ml @02:57:51.34 delay 3 seconds @02:57:54.35 AFV.select :carrierSolution @02:57:54.48 delay 2 seconds @02:57:56.51 AS.reconfigure ASconfig @02:57:56.59 AS.seek 1ml @02:58:54.39 delay 1 second @02:58:55.40 AFV.select :holdingCoil @02:58:55.52 delay 1 second @02:58:56.55 AS.reconfigure ASfast @02:58:56.61 AS.seek 0ml @02:59:23.98 delay 3 seconds @02:59:27.00 AFV.select :carrierSolution @02:59:27.12 delay 2 seconds @02:59:29.15 AS.reconfigure ASconfig @02:59:29.23 AS.seek 1ml @03:00:27.50 delay 1 second @03:00:28.52 AFV.select :holdingCoil @03:00:28.64 delay 1 second @03:00:29.67 AS.reconfigure ASnorm @03:00:29.74 AS.seek 0ml @03:01:20.49
AFV.select :carrierSolution @03:01:20.60 delay 2 seconds @03:01:22.63 AS.reconfigure ASconfig @03:01:22.70 AS.seek 0.5ml @03:01:51.78 delay 1 second @03:01:52.80 AFV.select :holdingCoil @03:01:52.92 ATV.dial :air1 @03:01:54.44 AS.reconfigure ASslow1 @03:01:54.50 AS.seek 0.52ml @03:01:58.81 delay 5 seconds @03:02:03.84 ATV.dial :extSrc @03:02:05.56 AS.reconfigure ASslow2 @03:02:05.64 AS.seek 0.538ml @03:02:23.95 delay 10 seconds @03:02:33.98 ATV.dial :air1 @03:02:35.72 AS.reconfigure ASslow1 @03:02:35.79 AS.seek 0.568ml @03:02:42.01 ATV.dial :waste @03:02:42.58 AS.reconfigure ASconfig @03:02:42.64 AS.seek :empty @03:03:15.83 Setting up PCR for ML -- writing /var/log/esp/ML20jul3003h.pcr @03:03:15.86 Building PCR Slug from elutionWater with MM2 mix, PP4 primer @03:03:15.88 Priming with carrier @03:03:15.90 ATV.dial ARV @03:03:17.42 ARV.dial :carrier @03:03:17.78 AS.reconfigure ASnorm @03:03:17.86 AS.seek 0.499ml @03:03:43.44 AFV.select :carrierSolution @03:03:43.58 delay 2 seconds @03:03:45.61 AS.reconfigure ASconfig @03:03:45.68 AS.seek 1ml @03:04:14.76 delay 1 second @03:04:15.78 AFV.select :holdingCoil @03:04:15.90 delay 1 second @03:04:16.93 ATV.dial :waste @03:04:18.62 AS.reconfigure ASnorm @03:04:18.70 AS.seek :empty @03:05:09.46 AFV.select :carrierSolution @03:05:09.60 delay 2 seconds @03:05:11.63 AS.reconfigure ASconfig @03:05:11.70 AS.seek 0.5ml @03:05:40.83 delay 1 second @03:05:41.85 AFV.select :holdingCoil @03:05:41.97 Building Slug @03:05:41.98 ATV.dial :ARV @03:05:43.65 delay 2 seconds @03:05:45.68 ARV.dial :air1 @03:05:46.09 delay 2 seconds @03:05:48.16 AS.reconfigure ASslow2 @03:05:48.23 AS.seek 0.506ml @03:05:54.42 delay 2 seconds @03:05:56.44 ARV.dial :MM2 @03:05:57.97 delay 2 seconds @03:06:00.00 Adding MM2 @03:06:00.09 AS.seek 0.524ml @03:06:18.44 delay 25 seconds @03:06:43.47 ARV.dial :PP4 @03:06:43.89 Adding PP4 @03:06:43.91 delay 2 seconds @03:06:46.00 AS.seek 0.53ml @03:06:52.22 delay 10 seconds @03:07:02.25 ARV.dial :air3 @03:07:02.65 adding ARV air @03:07:02.66 delay 2 seconds @03:07:04.76 AS.seek 0.536ml @03:07:11.03 delay 5 seconds @03:07:16.06 ARV.dial :carrier @03:07:17.36 delay 7 seconds @03:07:24.45 AS.seek 0.594ml @03:08:23.08 delay 5 seconds @03:08:28.11 ATV.dial :elutionWater @03:08:29.58 delay 2 seconds @03:08:31.67 AS.seek 0.6ml @03:08:38.12 delay 4 seconds @03:08:42.15 ATV.dial ARV @03:08:43.60 delay 2 seconds @03:08:45.63 ARV.dial :carrier @03:08:45.64 delay 2 seconds @03:08:47.74 AS.seek 0.65ml @03:09:38.45 delay 4 seconds @03:09:42.48 ATV.dial PCR @03:09:43.26 ASV.dial :waste1 @03:09:43.27 ARV.dial :air1 @03:09:43.71 Positioning Slug by 0.324ml @03:09:43.82 AS.seek 0.326ml @03:15:09.90 delay 5 seconds @03:15:14.93 ATV.dial ARV @03:15:15.73 ASV.dial :PCRlock @03:15:16.14 delay 5 seconds @03:15:21.17 Running ML Profile60C350 thermocycling @03:15:21.63 PCR.program Profile60C350 @03:15:21.76 Profile60C350 should complete in < 1:38:55 @03:15:21.78 PCR.run 2:08:38.75 @04:36:57.81 PCR.off @04:36:58.85 PCR for ML completed -- closed /var/log/esp/ML20jul3003h.pcr @04:36:58.94 Cleaning PCR @04:36:58.95 AFV.select :carrierSolution @04:36:59.08 delay 2 seconds @04:37:01.11 AS.reconfigure ASconfig @04:37:01.18 AS.seek 0.5ml @04:37:11.51 delay 1 second @04:37:12.52 AFV.select :holdingCoil @04:37:12.64 ASV.dial :waste1 @04:37:13.04 ATV.dial :air3 @04:37:14.37 AS.reconfigure ASslow1 @04:37:14.45 AS.seek 0.51ml @04:37:16.74 delay 2 seconds @04:37:18.76 ATV.dial :bleach @04:37:19.41 AS.seek 0.81ml @04:38:20.04 delay 3 seconds @04:38:23.06 ATV.dial :air3 @04:38:23.62 delay 3 seconds @04:38:26.70 AS.seek 0.835ml @04:38:32.01 delay 3 seconds @04:38:35.04 ATV.dial :bleach @04:38:35.67 AS.seek 0.82ml @04:38:38.99 delay 3 seconds @04:38:42.01 ARV.dial :waste @04:38:42.63 delay 3 seconds @04:38:45.66 ATV.dial :ARV @04:38:46.57 AS.reconfigure ASconfig @04:38:46.64 AS.seek 0.47ml @04:39:07.06 AFV.select :carrierSolution @04:39:07.19 delay 2 seconds @04:39:09.22 AS.seek 1ml @04:39:40.02 delay 1 second @04:39:41.03 AFV.select :holdingCoil @04:39:41.16 delay 1 second @04:39:42.19 AS.reconfigure ASfast @04:39:42.26 AS.seek 0ml @04:40:09.62 AFV.select :carrierSolution @04:40:09.76 delay 2 seconds @04:40:11.79 AS.reconfigure ASconfig @04:40:11.86 AS.seek 1ml @04:41:09.66 delay 1 second @04:41:10.67 AFV.select :holdingCoil @04:41:10.79 delay 1 second @04:41:11.82 AS.reconfigure ASfast @04:41:11.89 AS.seek 0ml @04:41:39.25 AFV.select :carrierSolution @04:41:39.38 delay 2 seconds @04:41:41.41 AS.reconfigure ASconfig @04:41:41.48 AS.seek 1ml @04:42:39.32 delay 1 second @04:42:40.33 AFV.select :holdingCoil @04:42:40.46 delay 1 second @04:42:41.49 AS.reconfigure ASfast @04:42:41.56 AS.seek 0ml @04:43:09.41 ARV.dial between carrier and air1 @04:43:09.92 ATV.dial :air3 @04:43:11.24 AS.reconfigure ASslow1 @04:43:11.31 AS.seek 0.01ml @04:43:13.79 delay 2 seconds @04:43:15.81 ATV.dial :bleach @04:43:16.47 AS.seek 0.81ml @04:45:57.66 delay 3 seconds @04:46:00.69 ATV.dial :air3 @04:46:01.26 delay 3 seconds @04:46:04.35 AS.seek 0.835ml @04:46:09.66 delay 3 seconds @04:46:12.69 ATV.dial :bleach @04:46:13.31 AS.seek 0.82ml @04:46:16.59 delay 3 seconds @04:46:19.62 ATV.dial PCR @04:46:20.04 AS.reconfigure ASconfig @04:46:20.10 AS.seek :empty @04:47:07.58 PCR.program :bleachProfile @04:47:07.65 PCR program should complete in < 2:45 @04:47:07.67 PCR.run 10:00 @04:50:00.42 PCR.off @04:50:00.46 AFV.select :carrierSolution @04:50:00.58 delay 2 seconds @04:50:02.61 AS.seek 1ml @04:51:00.43 delay 1 second @04:51:01.44 AFV.select :holdingCoil @04:51:01.57 delay 1 second @04:51:02.60 AS.reconfigure ASfast @04:51:02.67 AS.seek 0ml @04:51:30.06 delay 3 seconds @04:51:33.09 AFV.select :carrierSolution @04:51:33.21 delay 2 seconds @04:51:35.24 AS.reconfigure ASconfig @04:51:35.32 AS.seek 1ml @04:52:33.13 delay 1 second @04:52:34.14 AFV.select :holdingCoil @04:52:34.26 delay 1 second @04:52:35.29 AS.reconfigure ASfast @04:52:35.36 AS.seek 0ml @04:53:02.73 delay 3 seconds @04:53:05.75 AFV.select :carrierSolution @04:53:05.88 delay 2 seconds @04:53:07.91 AS.reconfigure ASconfig @04:53:07.98 AS.seek 1ml @04:54:05.79 delay 1 second @04:54:06.80 AFV.select :holdingCoil @04:54:06.93 delay 1 second @04:54:07.96 AS.reconfigure ASfast @04:54:08.03 AS.seek 0ml @04:54:35.40 delay 3 seconds @04:54:38.42 AFV.select :carrierSolution @04:54:38.55 delay 2 seconds @04:54:40.58 AS.reconfigure ASconfig @04:54:40.66 AS.seek 1ml @04:55:38.48 delay 1 second @04:55:39.49 AFV.select :holdingCoil @04:55:39.61 delay 1 second @04:55:40.64 AS.reconfigure ASfast @04:55:40.71 AS.seek 0ml @04:56:08.09 delay 3 seconds @04:56:11.12 AFV.select :carrierSolution @04:56:11.24 delay 2 seconds @04:56:13.27 AS.reconfigure ASconfig @04:56:13.35 AS.seek 1ml @04:57:11.16 delay 1 second @04:57:12.17 AFV.select :holdingCoil @04:57:12.29 delay 1 second @04:57:13.32 AS.reconfigure ASnorm @04:57:13.39 AS.seek 0ml @04:58:04.11
AFV.select :carrierSolution @04:58:04.24 delay 2 seconds @04:58:06.27 AS.reconfigure ASconfig @04:58:06.34 AS.seek 0.5ml @04:58:35.41 delay 1 second @04:58:36.42 AFV.select :holdingCoil @04:58:36.54 ATV.dial :air1 @04:58:38.06 AS.reconfigure ASslow2 @04:58:38.13 AS.seek 0.506ml @04:58:44.34 ATV.dial :extSrc @04:58:46.06 AS.seek 0.512ml @04:58:52.26 delay 5 seconds @04:58:57.29 ATV.dial :air1 @04:58:59.04 AS.reconfigure ASslow1 @04:58:59.11 AS.seek 0.542ml @04:59:05.41 ATV.dial :waste @04:59:05.98 AS.reconfigure ASconfig @04:59:06.04 AS.seek :empty @04:59:37.74 Setting up PCR for ML -- writing /var/log/esp/ML20jul3004h.pcr @04:59:37.77 Building PCR Slug from elutionWater with MM2 mix, PP4 primer @04:59:37.80 Priming with carrier @04:59:37.82 ATV.dial ARV @04:59:39.35 ARV.dial :carrier @04:59:39.72 AS.reconfigure ASnorm @04:59:39.80 AS.seek 0.499ml @05:00:05.39 AFV.select :carrierSolution @05:00:05.53 delay 2 seconds @05:00:07.93 AS.reconfigure ASconfig @05:00:08.08 AS.seek 1ml @05:00:37.16 delay 1 second @05:00:38.18 AFV.select :holdingCoil @05:00:38.30 delay 1 second @05:00:39.33 ATV.dial :waste @05:00:41.02 AS.reconfigure ASnorm @05:00:41.10 AS.seek :empty @05:01:31.86 AFV.select :carrierSolution @05:01:32.00 delay 2 seconds @05:01:34.03 AS.reconfigure ASconfig @05:01:34.10 AS.seek 0.5ml @05:02:03.20 delay 1 second @05:02:04.22 AFV.select :holdingCoil @05:02:04.34 Building Slug @05:02:04.35 ATV.dial :ARV @05:02:06.04 delay 2 seconds @05:02:08.07 ARV.dial :air1 @05:02:08.47 delay 2 seconds @05:02:10.55 AS.reconfigure ASslow2 @05:02:10.63 AS.seek 0.506ml @05:02:16.90 delay 2 seconds @05:02:18.92 ARV.dial :MM2 @05:02:20.45 delay 2 seconds @05:02:22.48 Adding MM2 @05:02:22.58 AS.seek 0.524ml @05:02:40.90 delay 25 seconds @05:03:05.93 ARV.dial :PP4 @05:03:06.34 Adding PP4 @05:03:06.36 delay 2 seconds @05:03:08.45 AS.seek 0.53ml @05:03:14.63 delay 10 seconds @05:03:24.66 ARV.dial :air3 @05:03:25.07 adding ARV air @05:03:25.09 delay 2 seconds @05:03:27.19 AS.seek 0.536ml @05:03:33.46 delay 5 seconds @05:03:38.49 ARV.dial :carrier @05:03:39.79 delay 7 seconds @05:03:46.88 AS.seek 0.594ml @05:04:45.44 delay 5 seconds @05:04:50.47 ATV.dial :elutionWater @05:04:51.95 delay 2 seconds @05:04:54.04 AS.seek 0.6ml @05:05:00.26 delay 4 seconds @05:05:04.29 ATV.dial ARV @05:05:05.75 delay 2 seconds @05:05:07.78 ARV.dial :carrier @05:05:07.79 delay 2 seconds @05:05:09.89 AS.seek 0.65ml @05:06:00.41 delay 4 seconds @05:06:04.44 ATV.dial PCR @05:06:05.23 ASV.dial :waste1 @05:06:05.24 ARV.dial :air1 @05:06:05.69 Positioning Slug by 0.324ml @05:06:05.80 AS.seek 0.326ml @05:11:32.12 delay 5 seconds @05:11:37.15 ATV.dial ARV @05:11:37.94 ASV.dial :PCRlock @05:11:38.34 delay 5 seconds @05:11:43.37 Running ML Profile60C350 thermocycling @05:11:43.38 PCR.program Profile60C350 @05:11:43.51 Profile60C350 should complete in < 1:38:55 @05:11:43.52 PCR.run 2:08:38.75 @06:32:58.43 PCR.off @06:32:59.48 PCR for ML completed -- closed /var/log/esp/ML20jul3004h.pcr @06:32:59.57 Cleaning PCR @06:32:59.58 AFV.select :carrierSolution @06:32:59.72 delay 2 seconds @06:33:01.75 AS.reconfigure ASconfig @06:33:01.82 AS.seek 0.5ml @06:33:12.17 delay 1 second @06:33:13.19 AFV.select :holdingCoil @06:33:13.31 ASV.dial :waste1 @06:33:13.71 ATV.dial :air3 @06:33:15.03 AS.reconfigure ASslow1 @06:33:15.11 AS.seek 0.51ml @06:33:17.40 delay 2 seconds @06:33:19.42 ATV.dial :bleach @06:33:20.07 AS.seek 0.81ml @06:34:20.69 delay 3 seconds @06:34:23.72 ATV.dial :air3 @06:34:24.28 delay 3 seconds @06:34:27.36 AS.seek 0.835ml @06:34:32.73 delay 3 seconds @06:34:35.76 ATV.dial :bleach @06:34:36.40 AS.seek 0.82ml @06:34:39.72 delay 3 seconds @06:34:42.74 ARV.dial :waste @06:34:43.38 delay 3 seconds @06:34:46.41 ATV.dial :ARV @06:34:47.31 AS.reconfigure ASconfig @06:34:47.38 AS.seek 0.47ml @06:35:07.79 AFV.select :carrierSolution @06:35:07.93 delay 2 seconds @06:35:09.96 AS.seek 1ml @06:35:40.72 delay 1 second @06:35:41.73 AFV.select :holdingCoil @06:35:41.86 delay 1 second @06:35:42.89 AS.reconfigure ASfast @06:35:42.96 AS.seek 0ml @06:36:10.32 AFV.select :carrierSolution @06:36:10.46 delay 2 seconds @06:36:12.49 AS.reconfigure ASconfig @06:36:12.56 AS.seek 1ml @06:37:10.31 delay 1 second @06:37:11.33 AFV.select :holdingCoil @06:37:11.45 delay 1 second @06:37:12.48 AS.reconfigure ASfast @06:37:12.55 AS.seek 0ml @06:37:39.91 AFV.select :carrierSolution @06:37:40.05 delay 2 seconds @06:37:42.08 AS.reconfigure ASconfig @06:37:42.15 AS.seek 1ml @06:38:39.91 delay 1 second @06:38:40.92 AFV.select :holdingCoil @06:38:41.04 delay 1 second @06:38:42.07 AS.reconfigure ASfast @06:38:42.14 AS.seek 0ml @06:39:09.51 ARV.dial between carrier and air1 @06:39:10.05 ATV.dial :air3 @06:39:11.36 AS.reconfigure ASslow1 @06:39:11.43 AS.seek 0.01ml @06:39:13.86 delay 2 seconds @06:39:15.88 ATV.dial :bleach @06:39:16.51 AS.seek 0.81ml @06:41:57.71 delay 3 seconds @06:42:00.73 ATV.dial :air3 @06:42:01.28 delay 3 seconds @06:42:04.37 AS.seek 0.835ml @06:42:09.70 delay 3 seconds @06:42:12.73 ATV.dial :bleach @06:42:13.37 AS.seek 0.82ml @06:42:16.67 delay 3 seconds @06:42:19.69 ATV.dial PCR @06:42:20.11 AS.reconfigure ASconfig @06:42:20.18 AS.seek :empty @06:43:07.65 PCR.program :bleachProfile @06:43:07.72 PCR program should complete in < 2:45 @06:43:07.74 PCR.run 10:00 @06:46:00.94 PCR.off @06:46:00.98 AFV.select :carrierSolution @06:46:01.11 delay 2 seconds @06:46:03.14 AS.seek 1ml @06:47:01.02 delay 1 second @06:47:02.04 AFV.select :holdingCoil @06:47:02.16 delay 1 second @06:47:03.19 AS.reconfigure ASfast @06:47:03.26 AS.seek 0ml @06:47:30.64 delay 3 seconds @06:47:33.66 AFV.select :carrierSolution @06:47:33.79 delay 2 seconds @06:47:35.82 AS.reconfigure ASconfig @06:47:35.89 AS.seek 1ml @06:48:33.71 delay 1 second @06:48:34.73 AFV.select :holdingCoil @06:48:34.85 delay 1 second @06:48:35.88 AS.reconfigure ASfast @06:48:35.94 AS.seek 0ml @06:49:03.31 delay 3 seconds @06:49:06.34 AFV.select :carrierSolution @06:49:06.46 delay 2 seconds @06:49:08.49 AS.reconfigure ASconfig @06:49:08.57 AS.seek 1ml @06:50:06.38 delay 1 second @06:50:07.39 AFV.select :holdingCoil @06:50:07.52 delay 1 second @06:50:08.55 AS.reconfigure ASfast @06:50:08.62 AS.seek 0ml @06:50:35.99 delay 3 seconds @06:50:39.02 AFV.select :carrierSolution @06:50:39.14 delay 2 seconds @06:50:41.17 AS.reconfigure ASconfig @06:50:41.25 AS.seek 1ml @06:51:39.05 delay 1 second @06:51:40.06 AFV.select :holdingCoil @06:51:40.19 delay 1 second @06:51:41.22 AS.reconfigure ASfast @06:51:41.29 AS.seek 0ml @06:52:08.66 delay 3 seconds @06:52:11.68 AFV.select :carrierSolution @06:52:11.81 delay 2 seconds @06:52:13.84 AS.reconfigure ASconfig @06:52:13.91 AS.seek 1ml @06:53:11.72 delay 1 second @06:53:12.73 AFV.select :holdingCoil @06:53:12.85 delay 1 second @06:53:13.88 AS.reconfigure ASnorm @06:53:13.95 AS.seek 0ml @06:54:04.69
deconEluateRecoveryLine extSrc @06:54:04.70 ATV.dial :waste @06:54:05.98 AS.seek 0ml @06:54:06.06 ATV.dial :air3 @06:54:06.94 AS.reconfigure ASslow1 @06:54:07.01 AS.seek 0.01ml @06:54:09.38 delay 2 seconds @06:54:11.40 ATV.dial :bleach @06:54:11.88 AS.seek 0.31ml @06:55:12.50 delay 3 seconds @06:55:15.52 ATV.dial :air3 @06:55:16.10 delay 3 seconds @06:55:19.17 AS.seek 0.335ml @06:55:24.50 delay 3 seconds @06:55:27.52 ATV.dial :bleach @06:55:28.17 AS.seek 0.32ml @06:55:31.49 delay 3 seconds @06:55:34.51 ATV.dial :extSrc @06:55:35.14 AS.seek 0ml @06:56:39.79 AFV.select :carrierSolution @06:56:39.92 delay 2 seconds @06:56:41.94 AS.reconfigure ASconfig @06:56:42.01 AS.seek 0.5ml @06:57:11.08 delay 1 second @06:57:12.10 AFV.select :holdingCoil @06:57:12.22 ATV.dial :extSrc @06:57:12.24 AS.reconfigure ASslow1 @06:57:12.32 AS.seek 0ml @06:58:53.15 AFV.select :carrierSolution @06:58:53.28 delay 2 seconds @06:58:55.30 AS.reconfigure ASconfig @06:58:55.36 AS.seek 0.5ml @06:59:24.44 delay 1 second @06:59:25.45 AFV.select :holdingCoil @06:59:25.58 ATV.dial :extSrc @06:59:25.60 AS.reconfigure ASslow1 @06:59:25.68 AS.seek 0ml @07:01:06.54 AFV.select :carrierSolution @07:01:06.67 delay 2 seconds @07:01:08.69 AS.reconfigure ASconfig @07:01:08.76 AS.seek 0.5ml @07:01:37.81 delay 1 second @07:01:38.82 AFV.select :holdingCoil @07:01:38.94 ATV.dial :extSrc @07:01:38.96 AS.reconfigure ASslow1 @07:01:39.04 AS.seek 0ml @07:03:19.88 AFV.select :carrierSolution @07:03:20.01 delay 2 seconds @07:03:22.04 AS.reconfigure ASconfig @07:03:22.11 AS.seek 0.5ml @07:03:51.16 delay 1 second @07:03:52.17 AFV.select :holdingCoil @07:03:52.30 ATV.dial :extSrc @07:03:52.32 AS.reconfigure ASslow1 @07:03:52.40 AS.seek 0ml @07:05:33.23 ATV.dial :air2 @07:05:34.61 AS.reconfigure ASslow0 @07:05:34.68 AS.seek 0.5ml @07:06:25.35 ATV.dial :extSrc @07:06:26.88 AS.seek 0.02ml @07:07:15.50 delay 1 second @07:07:16.52 ATV.dial :waste @07:07:17.84 AS.reconfigure ASconfig @07:07:17.90 AS.seek :empty @07:07:19.42 deconEluateRecoveryLine extSrc @07:07:19.43 ATV.dial :waste @07:07:19.47 AS.reconfigure ASnorm @07:07:19.54 AS.seek 0ml @07:07:19.91 ATV.dial :air3 @07:07:20.80 AS.reconfigure ASslow1 @07:07:21.33 AS.seek 0.01ml @07:07:23.61 delay 2 seconds @07:07:25.63 ATV.dial :bleach @07:07:26.12 AS.seek 0.31ml @07:08:26.74 delay 3 seconds @07:08:29.76 ATV.dial :air3 @07:08:30.33 delay 3 seconds @07:08:33.42 AS.seek 0.335ml @07:08:38.76 delay 3 seconds @07:08:41.78 ATV.dial :bleach @07:08:42.41 AS.seek 0.32ml @07:08:45.74 delay 3 seconds @07:08:48.76 ATV.dial :extSrc @07:08:49.40 AS.seek 0ml @07:09:54.02 AFV.select :carrierSolution @07:09:54.15 delay 2 seconds @07:09:56.17 AS.reconfigure ASconfig @07:09:56.24 AS.seek 0.5ml @07:10:25.30 delay 1 second @07:10:26.32 AFV.select :holdingCoil @07:10:26.44 ATV.dial :extSrc @07:10:26.46 AS.reconfigure ASslow1 @07:10:26.54 AS.seek 0ml @07:12:07.38 AFV.select :carrierSolution @07:12:07.51 delay 2 seconds @07:12:09.54 AS.reconfigure ASconfig @07:12:09.61 AS.seek 0.5ml @07:12:38.66 delay 1 second @07:12:39.67 AFV.select :holdingCoil @07:12:39.80 ATV.dial :extSrc @07:12:39.82 AS.reconfigure ASslow1 @07:12:39.90 AS.seek 0ml @07:14:20.76 AFV.select :carrierSolution @07:14:20.89 delay 2 seconds @07:14:22.91 AS.reconfigure ASconfig @07:14:22.98 AS.seek 0.5ml @07:14:52.03 delay 1 second @07:14:53.05 AFV.select :holdingCoil @07:14:53.17 ATV.dial :extSrc @07:14:53.19 AS.reconfigure ASslow1 @07:14:53.27 AS.seek 0ml @07:16:34.12 AFV.select :carrierSolution @07:16:34.25 delay 2 seconds @07:16:36.27 AS.reconfigure ASconfig @07:16:36.34 AS.seek 0.5ml @07:17:05.38 delay 1 second @07:17:06.40 AFV.select :holdingCoil @07:17:06.52 ATV.dial :extSrc @07:17:06.54 AS.reconfigure ASslow1 @07:17:06.62 AS.seek 0ml @07:18:47.47 ATV.dial :air2 @07:18:48.84 AS.reconfigure ASslow0 @07:18:48.91 AS.seek 0.5ml @07:19:39.52 ATV.dial :extSrc @07:19:41.05 AS.seek 0.02ml @07:20:29.67 delay 1 second @07:20:30.69 ATV.dial :waste @07:20:32.00 AS.reconfigure ASconfig @07:20:32.07 AS.seek :empty @07:20:33.59 deconEluateRecoveryLine extSrc @07:20:33.60 ATV.dial :waste @07:20:33.64 AS.reconfigure ASnorm @07:20:33.71 AS.seek 0ml @07:20:34.02 ATV.dial :air3 @07:20:34.90 AS.reconfigure ASslow1 @07:20:34.97 AS.seek 0.01ml @07:20:37.27 delay 2 seconds @07:20:39.29 ATV.dial :bleach @07:20:39.77 AS.seek 0.31ml @07:21:40.39 delay 3 seconds @07:21:43.41 ATV.dial :air3 @07:21:43.98 delay 3 seconds @07:21:47.05 AS.seek 0.335ml @07:21:52.40 delay 3 seconds @07:21:55.42 ATV.dial :bleach @07:21:56.05 AS.seek 0.32ml @07:21:59.45 delay 3 seconds @07:22:02.47 ATV.dial :extSrc @07:22:03.09 AS.seek 0ml @07:23:07.72 AFV.select :carrierSolution @07:23:07.85 delay 2 seconds @07:23:09.87 AS.reconfigure ASconfig @07:23:09.94 AS.seek 0.5ml @07:23:38.98 delay 1 second @07:23:40.00 AFV.select :holdingCoil @07:23:40.12 ATV.dial :extSrc @07:23:40.14 AS.reconfigure ASslow1 @07:23:40.22 AS.seek 0ml @07:25:21.07 AFV.select :carrierSolution @07:25:21.20 delay 2 seconds @07:25:23.22 AS.reconfigure ASconfig @07:25:23.29 AS.seek 0.5ml @07:25:52.33 delay 1 second @07:25:53.34 AFV.select :holdingCoil @07:25:53.47 ATV.dial :extSrc @07:25:53.49 AS.reconfigure ASslow1 @07:25:53.57 AS.seek 0ml @07:27:34.41 AFV.select :carrierSolution @07:27:34.54 delay 2 seconds @07:27:36.57 AS.reconfigure ASconfig @07:27:36.63 AS.seek 0.5ml @07:28:05.69 delay 1 second @07:28:06.70 AFV.select :holdingCoil @07:28:06.83 ATV.dial :extSrc @07:28:06.85 AS.reconfigure ASslow1 @07:28:06.93 AS.seek 0ml @07:29:47.79 AFV.select :carrierSolution @07:29:47.92 delay 2 seconds @07:29:49.94 AS.reconfigure ASconfig @07:29:50.01 AS.seek 0.5ml @07:30:19.06 delay 1 second @07:30:20.07 AFV.select :holdingCoil @07:30:20.20 ATV.dial :extSrc @07:30:20.22 AS.reconfigure ASslow1 @07:30:20.30 AS.seek 0ml @07:32:01.58 ATV.dial :air2 @07:32:02.95 AS.reconfigure ASslow0 @07:32:03.02 AS.seek 0.5ml @07:32:53.65 ATV.dial :extSrc @07:32:55.18 AS.seek 0.02ml @07:33:43.81 delay 1 second @07:33:44.83 ATV.dial :waste @07:33:46.14 AS.reconfigure ASconfig @07:33:46.21 AS.seek :empty @07:33:47.77 Session Ended